LOCUS       DAZGJT010000001        96968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZGJT010000001 DAZGJT010000000
VERSION     DAZGJT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.001"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            179..1750
                     /gene="garD"
                     /locus_tag="ACHKFK_000001"
     CDS             179..1750
                     /gene="garD"
                     /locus_tag="ACHKFK_000001"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO9050442.1"
                     /translation="MANIKIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAECHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1899..2234
                     /gene="prlF"
                     /locus_tag="ACHKFK_000002"
     CDS             1899..2234
                     /gene="prlF"
                     /locus_tag="ACHKFK_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO9050443.1"
                     /translation="MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFDIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2234..2698
                     /gene="yhaV"
                     /locus_tag="ACHKFK_000003"
     CDS             2234..2698
                     /gene="yhaV"
                     /locus_tag="ACHKFK_000003"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO9050444.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKA
                     ATKLLAVVHKVIEEYITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2753..3562)
                     /gene="agaR"
                     /locus_tag="ACHKFK_000004"
     CDS             complement(2753..3562)
                     /gene="agaR"
                     /locus_tag="ACHKFK_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO9050445.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVVERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3811..5091
                     /gene="kbaZ"
                     /locus_tag="ACHKFK_000005"
     CDS             3811..5091
                     /gene="kbaZ"
                     /locus_tag="ACHKFK_000005"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO9050446.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLETAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKATALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5114..5587
                     /gene="agaV"
                     /locus_tag="ACHKFK_000006"
     CDS             5114..5587
                     /gene="agaV"
                     /locus_tag="ACHKFK_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO9050447.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDLVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5598..6377
                     /gene="agaW"
                     /locus_tag="ACHKFK_000007"
     CDS             5598..6377
                     /gene="agaW"
                     /locus_tag="ACHKFK_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO9050448.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6367..7245
                     /gene="agaE"
                     /locus_tag="ACHKFK_000008"
     CDS             6367..7245
                     /gene="agaE"
                     /locus_tag="ACHKFK_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO9050449.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7263..7697
                     /gene="agaF"
                     /locus_tag="ACHKFK_000009"
     CDS             7263..7697
                     /gene="agaF"
                     /locus_tag="ACHKFK_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO9050450.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTAQL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7694..8827
                     /gene="nagA"
                     /locus_tag="ACHKFK_000010"
     CDS             7694..8827
                     /gene="nagA"
                     /locus_tag="ACHKFK_000010"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9050451.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDALDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIALRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGHYTLCGEEVQMRGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9177..10331
                     /gene="agaS"
                     /locus_tag="ACHKFK_000011"
     CDS             9177..10331
                     /gene="agaS"
                     /locus_tag="ACHKFK_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO9050452.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLQIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVKLANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPEMINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESNDVIAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10344..11204
                     /gene="kbaY"
                     /locus_tag="ACHKFK_000012"
     CDS             10344..11204
                     /gene="kbaY"
                     /locus_tag="ACHKFK_000012"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO9050453.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11371..11847
                     /gene="agaB"
                     /locus_tag="ACHKFK_000013"
     CDS             11371..11847
                     /gene="agaB"
                     /locus_tag="ACHKFK_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO9050454.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDA
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDVNVGNMHFSEGKKQISSKVYVDDQDLTDLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11886..12689
                     /gene="agaC"
                     /locus_tag="ACHKFK_000014"
     CDS             11886..12689
                     /gene="agaC"
                     /locus_tag="ACHKFK_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO9050455.1"
                     /translation="MHEITLLQGLSLAALVFFLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12679..13470
                     /gene="agaD"
                     /locus_tag="ACHKFK_000015"
     CDS             12679..13470
                     /gene="agaD"
                     /locus_tag="ACHKFK_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO9050456.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13471..14238
                     /gene="agaI"
                     /locus_tag="ACHKFK_000016"
                     /pseudo
     CDS             13471..14238
                     /gene="agaI"
                     /locus_tag="ACHKFK_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843039.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
     gene            complement(14288..15151)
                     /gene="rsmI"
                     /locus_tag="ACHKFK_000017"
     CDS             complement(14288..15151)
                     /gene="rsmI"
                     /locus_tag="ACHKFK_000017"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO9050457.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQGE"
     gene            15216..17252
                     /gene="lpoA"
                     /locus_tag="ACHKFK_000018"
     CDS             15216..17252
                     /gene="lpoA"
                     /locus_tag="ACHKFK_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO9050458.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRIWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVVAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     SVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGPVLQQKFGSTSELRAGVNGGSGIALTGSPITPTETT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            17210..17605
                     /gene="yraN"
                     /locus_tag="ACHKFK_000019"
     CDS             17210..17605
                     /gene="yraN"
                     /locus_tag="ACHKFK_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO9050459.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTIFIEVRYRRSALYGGSAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            17625..18215
                     /gene="diaA"
                     /locus_tag="ACHKFK_000020"
     CDS             17625..18215
                     /gene="diaA"
                     /locus_tag="ACHKFK_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO9050460.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            18225..18800
                     /gene="dolP"
                     /locus_tag="ACHKFK_000021"
     CDS             18225..18800
                     /gene="dolP"
                     /locus_tag="ACHKFK_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO9050461.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(18913..19953)
                     /gene="yraQ"
                     /locus_tag="ACHKFK_000022"
     CDS             complement(18913..19953)
                     /gene="yraQ"
                     /locus_tag="ACHKFK_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO9050462.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPLLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPESQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLV"
     gene            complement(20026..20661)
                     /gene="yraR"
                     /locus_tag="ACHKFK_000023"
     CDS             complement(20026..20661)
                     /gene="yraR"
                     /locus_tag="ACHKFK_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO9050463.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            20789..21307
                     /gene="yhbO"
                     /locus_tag="ACHKFK_000024"
     CDS             20789..21307
                     /gene="yhbO"
                     /locus_tag="ACHKFK_000024"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO9050464.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIVIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(21287..21730)
                     /gene="yhbP"
                     /locus_tag="ACHKFK_000025"
     CDS             complement(21287..21730)
                     /gene="yhbP"
                     /locus_tag="ACHKFK_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO9050465.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAIAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRNSGTEQA"
     gene            21781..22083
                     /gene="yhbQ"
                     /locus_tag="ACHKFK_000026"
     CDS             21781..22083
                     /gene="yhbQ"
                     /locus_tag="ACHKFK_000026"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO9050466.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAVFAELLNSLQTPEIKSD"
     gene            complement(22070..22573)
                     /gene="yhbS"
                     /locus_tag="ACHKFK_000027"
     CDS             complement(22070..22573)
                     /gene="yhbS"
                     /locus_tag="ACHKFK_000027"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9050467.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(22567..23091)
                     /gene="ubiT"
                     /locus_tag="ACHKFK_000028"
     CDS             complement(22567..23091)
                     /gene="ubiT"
                     /locus_tag="ACHKFK_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO9050468.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            23300..24295
                     /gene="ubiU"
                     /locus_tag="ACHKFK_000029"
     CDS             23300..24295
                     /gene="ubiU"
                     /locus_tag="ACHKFK_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO9050469.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            24304..25182
                     /gene="ubiV"
                     /locus_tag="ACHKFK_000030"
     CDS             24304..25182
                     /gene="ubiV"
                     /locus_tag="ACHKFK_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO9050470.1"
                     /translation="MKYSLGPVLWYWPKETLEEFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQDTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            25263..26270
                     /gene="yhbW"
                     /locus_tag="ACHKFK_000031"
     CDS             25263..26270
                     /gene="yhbW"
                     /locus_tag="ACHKFK_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFO9050471.1"
                     /translation="MTDKTIPFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(26388..27632)
                     /gene="mtr"
                     /locus_tag="ACHKFK_000032"
     CDS             complement(26388..27632)
                     /gene="mtr"
                     /locus_tag="ACHKFK_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO9050472.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIMLIL
                     VFGVGNALVHILSSFNVLPVYQ"
     gene            complement(27786..29675)
                     /gene="deaD"
                     /locus_tag="ACHKFK_000033"
     CDS             complement(27786..29675)
                     /gene="deaD"
                     /locus_tag="ACHKFK_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO9050473.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRDTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(29668..29748)
                     /gene="yrbN"
                     /locus_tag="ACHKFK_000034"
     CDS             complement(29668..29748)
                     /gene="yrbN"
                     /locus_tag="ACHKFK_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO9050474.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(29855..30739)
                     /gene="nlpI"
                     /locus_tag="ACHKFK_000035"
     CDS             complement(29855..30739)
                     /gene="nlpI"
                     /locus_tag="ACHKFK_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO9050475.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARIEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(30848..32983)
                     /gene="pnp"
                     /locus_tag="ACHKFK_000036"
     CDS             complement(30848..32983)
                     /gene="pnp"
                     /locus_tag="ACHKFK_000036"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO9050476.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YNGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(33230..33499)
                     /gene="rpsO"
                     /locus_tag="ACHKFK_000037"
     CDS             complement(33230..33499)
                     /gene="rpsO"
                     /locus_tag="ACHKFK_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO9050477.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(33648..34592)
                     /gene="truB"
                     /locus_tag="ACHKFK_000038"
     CDS             complement(33648..34592)
                     /gene="truB"
                     /locus_tag="ACHKFK_000038"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO9050478.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(34592..34993)
                     /gene="rbfA"
                     /locus_tag="ACHKFK_000039"
     CDS             complement(34592..34993)
                     /gene="rbfA"
                     /locus_tag="ACHKFK_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO9050479.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(35157..37829)
                     /gene="infB"
                     /locus_tag="ACHKFK_000040"
     CDS             complement(35157..37829)
                     /gene="infB"
                     /locus_tag="ACHKFK_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO9050480.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAGVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(37854..39341)
                     /gene="nusA"
                     /locus_tag="ACHKFK_000041"
     CDS             complement(37854..39341)
                     /gene="nusA"
                     /locus_tag="ACHKFK_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO9050481.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(39369..39821)
                     /gene="rimP"
                     /locus_tag="ACHKFK_000042"
     CDS             complement(39369..39821)
                     /gene="rimP"
                     /locus_tag="ACHKFK_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO9050482.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(40028..40104)
                     /locus_tag="ACHKFK_000043"
     tRNA            complement(40028..40104)
                     /locus_tag="ACHKFK_000043"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40068..40070),aa:Met,seq:cat)
     gene            40452..41795
                     /gene="argG"
                     /locus_tag="ACHKFK_000044"
     CDS             40452..41795
                     /gene="argG"
                     /locus_tag="ACHKFK_000044"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO9050483.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSAASGVPQVENLENKGQ"
     gene            complement(41803..43428)
                     /gene="yhbX"
                     /locus_tag="ACHKFK_000045"
     CDS             complement(41803..43428)
                     /gene="yhbX"
                     /locus_tag="ACHKFK_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9050484.1"
                     /translation="MTVFNKFVKSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRILSLPLRLLIAVPFVLLTAADMSISLYSWCIFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFTFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKEAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYLDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDCRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVLLRKEFTEDNQGNPTPEGQG"
     gene            complement(43885..43971)
                     /locus_tag="ACHKFK_000046"
     tRNA            complement(43885..43971)
                     /locus_tag="ACHKFK_000046"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43935..43937),aa:Leu,seq:gag)
     gene            complement(43986..44318)
                     /gene="secG"
                     /locus_tag="ACHKFK_000047"
     CDS             complement(43986..44318)
                     /gene="secG"
                     /locus_tag="ACHKFK_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO9050485.1"
                     /translation="MYEALLVVFLIVATGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(44546..45883)
                     /gene="glmM"
                     /locus_tag="ACHKFK_000048"
     CDS             complement(44546..45883)
                     /gene="glmM"
                     /locus_tag="ACHKFK_000048"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO9050486.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(45876..46724)
                     /gene="folP"
                     /locus_tag="ACHKFK_000049"
     CDS             complement(45876..46724)
                     /gene="folP"
                     /locus_tag="ACHKFK_000049"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO9050487.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(46814..48748)
                     /gene="ftsH"
                     /locus_tag="ACHKFK_000050"
     CDS             complement(46814..48748)
                     /gene="ftsH"
                     /locus_tag="ACHKFK_000050"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO9050488.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(48848..49477)
                     /gene="rlmE"
                     /locus_tag="ACHKFK_000051"
     CDS             complement(48848..49477)
                     /gene="rlmE"
                     /locus_tag="ACHKFK_000051"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO9050489.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            49603..49896
                     /gene="yhbY"
                     /locus_tag="ACHKFK_000052"
     CDS             49603..49896
                     /gene="yhbY"
                     /locus_tag="ACHKFK_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO9050490.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(50052..50528)
                     /gene="greA"
                     /locus_tag="ACHKFK_000053"
     CDS             complement(50052..50528)
                     /gene="greA"
                     /locus_tag="ACHKFK_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO9050491.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            50775..52208
                     /gene="dacB"
                     /locus_tag="ACHKFK_000054"
     CDS             50775..52208
                     /gene="dacB"
                     /locus_tag="ACHKFK_000054"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9050492.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLRRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(52249..53421)
                     /gene="cgtA"
                     /locus_tag="ACHKFK_000055"
     CDS             complement(52249..53421)
                     /gene="cgtA"
                     /locus_tag="ACHKFK_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO9050493.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(53437..54402)
                     /gene="yhbE"
                     /locus_tag="ACHKFK_000056"
     CDS             complement(53437..54402)
                     /gene="yhbE"
                     /locus_tag="ACHKFK_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9050494.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLANPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(54529..54786)
                     /gene="rpmA"
                     /locus_tag="ACHKFK_000057"
     CDS             complement(54529..54786)
                     /gene="rpmA"
                     /locus_tag="ACHKFK_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO9050495.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(54807..55118)
                     /gene="rplU"
                     /locus_tag="ACHKFK_000058"
     CDS             complement(54807..55118)
                     /gene="rplU"
                     /locus_tag="ACHKFK_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO9050496.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            55377..56348
                     /gene="ispB"
                     /locus_tag="ACHKFK_000059"
     CDS             55377..56348
                     /gene="ispB"
                     /locus_tag="ACHKFK_000059"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO9050497.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            56576..56854
                     /gene="sfsB"
                     /locus_tag="ACHKFK_000060"
     CDS             56576..56854
                     /gene="sfsB"
                     /locus_tag="ACHKFK_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO9050498.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(56902..58161)
                     /gene="murA"
                     /locus_tag="ACHKFK_000061"
     CDS             complement(56902..58161)
                     /gene="murA"
                     /locus_tag="ACHKFK_000061"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO9050499.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(58216..58470)
                     /gene="ibaG"
                     /locus_tag="ACHKFK_000062"
     CDS             complement(58216..58470)
                     /gene="ibaG"
                     /locus_tag="ACHKFK_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO9050500.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(58630..58923)
                     /gene="mlaB"
                     /locus_tag="ACHKFK_000063"
     CDS             complement(58630..58923)
                     /gene="mlaB"
                     /locus_tag="ACHKFK_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO9050501.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEIREEAVKGITSIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(58923..59558)
                     /gene="mlaC"
                     /locus_tag="ACHKFK_000064"
     CDS             complement(58923..59558)
                     /gene="mlaC"
                     /locus_tag="ACHKFK_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO9050502.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQRDAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(59577..60128)
                     /gene="mlaD"
                     /locus_tag="ACHKFK_000065"
     CDS             complement(59577..60128)
                     /gene="mlaD"
                     /locus_tag="ACHKFK_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO9050503.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(60133..60915)
                     /gene="mlaE"
                     /locus_tag="ACHKFK_000066"
     CDS             complement(60133..60915)
                     /gene="mlaE"
                     /locus_tag="ACHKFK_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO9050504.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(60923..61732)
                     /gene="mlaF"
                     /locus_tag="ACHKFK_000067"
     CDS             complement(60923..61732)
                     /gene="mlaF"
                     /locus_tag="ACHKFK_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO9050505.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            61942..62919
                     /gene="yrbG"
                     /locus_tag="ACHKFK_000068"
     CDS             61942..62919
                     /gene="yrbG"
                     /locus_tag="ACHKFK_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO9050506.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPREGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFIIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            62933..63919
                     /gene="kdsD"
                     /locus_tag="ACHKFK_000069"
     CDS             62933..63919
                     /gene="kdsD"
                     /locus_tag="ACHKFK_000069"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO9050507.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            63940..64506
                     /gene="kdsC"
                     /locus_tag="ACHKFK_000070"
     CDS             63940..64506
                     /gene="kdsC"
                     /locus_tag="ACHKFK_000070"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO9050508.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTHIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            64503..65078
                     /gene="lptC"
                     /locus_tag="ACHKFK_000071"
     CDS             64503..65078
                     /gene="lptC"
                     /locus_tag="ACHKFK_000071"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO9050509.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTFYEIQNKQTQP"
     gene            65047..65604
                     /gene="lptA"
                     /locus_tag="ACHKFK_000072"
     CDS             65047..65604
                     /gene="lptA"
                     /locus_tag="ACHKFK_000072"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO9050510.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            65611..66336
                     /gene="lptB"
                     /locus_tag="ACHKFK_000073"
     CDS             65611..66336
                     /gene="lptB"
                     /locus_tag="ACHKFK_000073"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO9050511.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEYVKRVYLGEDFRL"
     gene            66384..67817
                     /gene="rpoN"
                     /locus_tag="ACHKFK_000074"
     CDS             66384..67817
                     /gene="rpoN"
                     /locus_tag="ACHKFK_000074"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO9050512.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            67840..68127
                     /gene="hpf"
                     /locus_tag="ACHKFK_000075"
     CDS             67840..68127
                     /gene="hpf"
                     /locus_tag="ACHKFK_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO9050513.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            68245..68736
                     /gene="ptsN"
                     /locus_tag="ACHKFK_000076"
     CDS             68245..68736
                     /gene="ptsN"
                     /locus_tag="ACHKFK_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO9050514.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LAPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            68782..69636
                     /gene="rapZ"
                     /locus_tag="ACHKFK_000077"
     CDS             68782..69636
                     /gene="rapZ"
                     /locus_tag="ACHKFK_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO9050515.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            69633..69905
                     /gene="npr"
                     /locus_tag="ACHKFK_000078"
     CDS             69633..69905
                     /gene="npr"
                     /locus_tag="ACHKFK_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO9050516.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            70119..70751
                     /gene="yrbL"
                     /locus_tag="ACHKFK_000079"
     CDS             70119..70751
                     /gene="yrbL"
                     /locus_tag="ACHKFK_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO9050517.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVTPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(70748..71476)
                     /gene="mtgA"
                     /locus_tag="ACHKFK_000080"
     CDS             complement(70748..71476)
                     /gene="mtgA"
                     /locus_tag="ACHKFK_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO9050518.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAIFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEQA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(71473..72126)
                     /gene="elbB"
                     /locus_tag="ACHKFK_000081"
     CDS             complement(71473..72126)
                     /gene="elbB"
                     /locus_tag="ACHKFK_000081"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO9050519.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAILPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(72356..74692)
                     /gene="arcB"
                     /locus_tag="ACHKFK_000082"
     CDS             complement(72356..74692)
                     /gene="arcB"
                     /locus_tag="ACHKFK_000082"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO9050520.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRALTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFERMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(74788..75717)
                     /gene="yhcC"
                     /locus_tag="ACHKFK_000083"
     CDS             complement(74788..75717)
                     /gene="yhcC"
                     /locus_tag="ACHKFK_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562163.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO9050521.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARERGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVGLDRYLNEHGVQGSALGRPWLPPTA"
     gene            76392..80852
                     /gene="gltB"
                     /locus_tag="ACHKFK_000084"
     CDS             76392..80852
                     /gene="gltB"
                     /locus_tag="ACHKFK_000084"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO9050522.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEDHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLLKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGADIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRGLMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESSDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            80865..82283
                     /gene="gltD"
                     /locus_tag="ACHKFK_000085"
     CDS             80865..82283
                     /gene="gltD"
                     /locus_tag="ACHKFK_000085"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO9050523.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAIVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGK
                     THDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEDNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            complement(82351..82815)
                     /gene="nanQ"
                     /locus_tag="ACHKFK_000086"
     CDS             complement(82351..82815)
                     /gene="nanQ"
                     /locus_tag="ACHKFK_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO9050524.1"
                     /translation="MMMGEVQSLPSAGLHPALQGALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(82812..83687)
                     /gene="nanK"
                     /locus_tag="ACHKFK_000087"
     CDS             complement(82812..83687)
                     /gene="nanK"
                     /locus_tag="ACHKFK_000087"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO9050525.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALDGDITDMVFITVSTGVGGGVVSGGKLRTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGANAKTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     TGLLGAALLAQGEIL"
     gene            complement(83684..84373)
                     /gene="nanE"
                     /locus_tag="ACHKFK_000088"
     CDS             complement(83684..84373)
                     /gene="nanE"
                     /locus_tag="ACHKFK_000088"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO9050526.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(84421..85911)
                     /gene="nanT"
                     /locus_tag="ACHKFK_000089"
     CDS             complement(84421..85911)
                     /gene="nanT"
                     /locus_tag="ACHKFK_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO9050527.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHGGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYDPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGISGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPILGALIAQRLDLGTALGSLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(86020..86913)
                     /gene="nanA"
                     /locus_tag="ACHKFK_000090"
     CDS             complement(86020..86913)
                     /gene="nanA"
                     /locus_tag="ACHKFK_000090"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO9050528.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(87035..87826)
                     /gene="nanR"
                     /locus_tag="ACHKFK_000091"
     CDS             complement(87035..87826)
                     /gene="nanR"
                     /locus_tag="ACHKFK_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO9050529.1"
                     /translation="MSPMNAFDPQAEDSTTTIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            complement(87934..88431)
                     /gene="sspB"
                     /locus_tag="ACHKFK_000092"
     CDS             complement(87934..88431)
                     /gene="sspB"
                     /locus_tag="ACHKFK_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO9050530.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDASIMNDEEASADNETVMSVIDGDKPDHDDETHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(88437..89075)
                     /gene="sspA"
                     /locus_tag="ACHKFK_000093"
     CDS             complement(88437..89075)
                     /gene="sspA"
                     /locus_tag="ACHKFK_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO9050531.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(89470..89862)
                     /gene="rpsI"
                     /locus_tag="ACHKFK_000094"
     CDS             complement(89470..89862)
                     /gene="rpsI"
                     /locus_tag="ACHKFK_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO9050532.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(89878..90306)
                     /gene="rplM"
                     /locus_tag="ACHKFK_000095"
     CDS             complement(89878..90306)
                     /gene="rplM"
                     /locus_tag="ACHKFK_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO9050533.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(90525..91652)
                     /gene="zapE"
                     /locus_tag="ACHKFK_000096"
     CDS             complement(90525..91652)
                     /gene="zapE"
                     /locus_tag="ACHKFK_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO9050534.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHMPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHDELTELQGQSDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDEIRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            91846..92244
                     /gene="zapG"
                     /locus_tag="ACHKFK_000097"
     CDS             91846..92244
                     /gene="zapG"
                     /locus_tag="ACHKFK_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO9050535.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            92398..93745
                     /gene="degQ"
                     /locus_tag="ACHKFK_000098"
                     /pseudo
     CDS             92398..93745
                     /gene="degQ"
                     /locus_tag="ACHKFK_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            93835..94902
                     /gene="degS"
                     /locus_tag="ACHKFK_000099"
     CDS             93835..94902
                     /gene="degS"
                     /locus_tag="ACHKFK_000099"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO9050536.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINPRRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGM
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVNNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(94965..95903)
                     /gene="mdh"
                     /locus_tag="ACHKFK_000100"
     CDS             complement(94965..95903)
                     /gene="mdh"
                     /locus_tag="ACHKFK_000100"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9050537.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QEVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            96338..96808
                     /gene="argR"
                     /locus_tag="ACHKFK_000101"
     CDS             96338..96808
                     /gene="argR"
                     /locus_tag="ACHKFK_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO9050538.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKELYEAILELFDQEL"
BASE COUNT        23692 a        26051 c        24311 g        22914 t
ORIGIN      
        1 taaagcacag atttgcgctt taccttgccg ggaggtactg caatccctga aatgattgac
       61 attgatcaca tttctgcatt taaactcctg acattcttat ttcacccaat gaagttattt
      121 atttttaaat gagaccaggt cctcatttta atagtccctg gctggagaat attgcacaat
      181 ggccaacatc aaaatcagac aagaaacgcc aactgcgttt tatataaaag ttcacgacac
      241 agataatgtg gcaattattg ttaatgataa tggcctgaaa gcaggaacgc gttttccgga
      301 tgggctggaa ttaattgaac atattcccca agggcataaa gtcgcattgc tggacattcc
      361 ggctaatggt gaaattattc gttatggcga ggtgattggt tacgccgtgc gcgcaatccc
      421 acgcggaagc tggatcgacg aatcaatggt tgtactaccg gaagcgccgc cgttacatac
      481 gctgccactg gcaaccaaag tcccggaacc cttaccgccg ctggaaggat acacctttga
      541 gggctatcgc aatgccgatg gcagcgtggg caccaaaaac ctgctcggca tcaccaccag
      601 cgtccactgt gtggcaggcg tggtggacta cgtagtaaaa atcattgaac gcgatctgct
      661 accgaaatac ccgaacgtcg atggcgtggt ggggctgaat catttgtacg gttgtggcgt
      721 ggcgattaac gcacccgcgg cagttgtacc tattcgtacc attcacaata tttcgctgaa
      781 ccctaacttt ggcggcgaag taatggtgat tggtctgggt tgtgaaaagt tgcagcctga
      841 acgcctactg actggaacgg atgatgtgca agctattcca gtagaaagcg ccagcattgt
      901 cagtttgcag gatgaaaagc atgtcggttt tcagtccatg gtcgaggata ttttgcaggt
      961 cgccgaatgc catctacaaa aactgaatca acgtcagcga gaaacctgtc cggcttcaga
     1021 actggttgtc ggcatgcagt gcggtggcag cgatgcgttt tctggcgtaa cggcaaaccc
     1081 ggcggttggg tatgcgtctg atctattggt gcgctgcggc gcaacggtga tgttttcaga
     1141 agtcacagaa gtgcgtgacg cgatacatct gctgacacca cgcgcagtga acgaagaggt
     1201 cggcaaacgg ctgctggagg agatggagtg gtacgataac tatctcaata tgggaaaaac
     1261 cgatcgcagc gccaaccctt cgccggggaa caagaaaggc ggtctggcaa atgtggtgga
     1321 gaaggcactc ggctccattg ctaaatcggg taaaagtgca attgttgaag tgctgtcgcc
     1381 cggccaacgc ccgactaaac gcggattaat ttacgccgcg acgccagcca gcgattttgt
     1441 ctgtggcacg caacaggtgg cttcgggtat caccgtgcaa gtgtttacga ccggtcgtgg
     1501 tacgccgtac ggcctgatgg cggtacccgt cattaaaatg gcgacccgca ccgagctggc
     1561 gaaccgctgg tttgatttaa tggatattaa cgcgggcact atcgctaccg gcgaagaaac
     1621 cattgaagaa gtgggctgga agttgtttca ctttattctc gacgtcgcca gcgggaagaa
     1681 gaaaactttc tcggatcaat ggggactgca taaccagctg gcggtgttta acccggcacc
     1741 ggtgacctga tttcttttgg aacgtccctc gcaaaacatg gccttagtgc catgttttta
     1801 ttgtttaaag tccacacatc cattaataat gcatttgcat taccttagtt caagcttata
     1861 atttgagcag aaaacaggct gtaaaaggac agtaaataat gcccgctaat gctcgctcta
     1921 acgctgtact gaccactgaa tcaaaggtca ctatacgcgg acaaacaact atccccgcgc
     1981 cagtgcgtga ggccttaaaa ctgaagcctg gcctggacag cattcattac gaaattctgc
     2041 ctggtgggca agtatttatg tgccgactgg gagatgaaca ggaggatcat actatgaatg
     2101 catttttgcg ttttctggat gcagatatcc agaacaaccc gcaaaaaact cgtccattcg
     2161 acattcaaca aggaaagaaa cttgtcgctg gcatggacgt caacattgac gatgagattg
     2221 gcgacgacga ataatggatt ttccacaaag ggttaatggt tgggcgctat atgctcatcc
     2281 ctgttttcag gaaacctacg atgctttagt tgccgaagtc gaggcattaa agggaaaaga
     2341 tcctgaaaat tatcagagaa aagctgccac aaagttattg gcggtggtcc ataaagtgat
     2401 tgaggagtat atcacggtca atccatcatc accggcattc cgtcatggca agtcgttagg
     2461 ctctgggaaa aataaagact ggtcacgggt aaaatttggc gctggtcgct atcgtctctt
     2521 ctttcgttac agtgaaaaag agaaagtcat cattctggga tggatgaacg atgaaaacac
     2581 gctgcgcacc tacggtaaaa aaacagatgc ctataccgta ttcagtaaaa tgttaaaaag
     2641 aggacatcct cctgccgact gggaaagcct cacccaagag acagaagaaa accattgatg
     2701 gtgttcacaa tgcccgccga acgcattggt gttgaccagc gggcatcctt tctcattccc
     2761 cgaccagaat cacttcaacc ccagcctttc tcagtccttc cagactatcc gcaggaatgc
     2821 cttcatcaac aatgatcatg tcgatacgtt gagtatcaat gatcttatgt aaactagaac
     2881 gattgaactt actggaatcg gtgaccacga tgatccgttc cacaacttca cacatccgac
     2941 ggtttaaacg ggcttcatct tcattatgcg tgctgacgcc gcgctccaga tcgatcgcat
     3001 ctacaccaag aaacagcata tcgaagtggt aattttgcag cgattgctca gcctgatcac
     3061 cgtaaaaaga ttgcgactga cggcgcaaat gccccccggt catcagcagc tcaacgcctt
     3121 ccgcttccag caacgcatta gccacgttca taccgttggt catcgcaatt acgtcagtgt
     3181 gcttgcgcat cagacgagca atctcaaaag tggtggtccc ggagtcgagg atcacccgat
     3241 gaccaggctg aatcaactca acggcagctt tcgcaacgct gcgtttcatc gcggtgttca
     3301 gcgcgctttt atcttccact gatggctcga ctgacggcgt tgtgctatcg cagatcaacg
     3361 cgccaccata ggcacgcaca gcgatcccct gcttttccag aaacgccaga tcgttgcgga
     3421 tcgtcacagt agatacacca tacaacgccg atagatcatt aacttgcaca cttccttgct
     3481 ggcgcaggcg ttgaatgatc tgttctcgtc gctcgctggt gcctgtcact cgcttctcac
     3541 ctgaagcgtc ggtattactc atagtaagtc ctttcgtaaa actttcgttt catttcgttt
     3601 tgcctattaa cgcctttcta ttaagcaaat gcaagcccac cttacccatt ggcgcaagct
     3661 actctcgttt cagtagcttt cattatgttt cttttgtgaa tcagatcaga aaactattat
     3721 ctttcgtttt atttttatct caccatgacg cagtatcaac tgaaacaaaa cgaaagatta
     3781 atgtcgcagt aatctgaact ggagaggaaa gtgaaacatc tgacagaaat ggtgagacag
     3841 cacaaagcgg gcaaaacaaa tgggatttat gccgtttgtt ccgcacatcc gctggtgctg
     3901 gaaactgcaa tccgctacgc cagtgcaaac caaacgccgt tactgattga agcaacctcc
     3961 aatcaggtgg accagttcgg cggttatacc ggaatgacgc ccgccgattt tcgcggcttt
     4021 gtttgtcagc tcgccgactc gttgaatttc ccgcaggatg cgctgattct gggtggcgac
     4081 catctggggc caaaccgctg gcaaaacctg cctgccgctc aggcaatggc caatgccgat
     4141 gatttgatta aaagctacgt ggcggcagga ttcaaaaaaa ttcaccttga ttgcagcatg
     4201 tcctgtcagg acgatccgat tcccttaact gatgacatcg tggctgaacg cgccgcccgt
     4261 ctggcgaaag tggcggaaga aacctgtctt gaacactttg gcgaagccga tctggagtat
     4321 gtcattggta ccgaagtccc ggtgcctggc ggcgcgcatg aaaccttaag cgagctggcg
     4381 gtcaccacgc cggatgccgc ccgcgccacg ctggaagccc atcgtcacgc ttttgaaaag
     4441 cagggtctga atgccatctg gccacgcatc attgccctgg tggttcaacc cggcgtcgaa
     4501 ttcgatcaca ccaacgttat tgattatcag cccgccaaag cgaccgcctt aagccagatg
     4561 gtcgaaaact acgaaacgct gattttcgaa gcgcactcta ccgattatca aacaccgcaa
     4621 tcgctgcgcc agctggtgat tgaccacttt gccattctga aagttggccc ggcgctgacc
     4681 tttgctctgc gtgaagctct gttctctctg gcggcgattg aagaagaact ggtgccagca
     4741 aaagcctgtt ctggtctgcg tcaggtgctg gaaaacgtga tgctcgaccg cccggaatac
     4801 tggcaaagcc actaccacgg tgacggcaac gcgcgtcgtc tggcgcgtgg ttatagctac
     4861 tcggatcgcg tgcgctatta ctggccggac agccagattg atgacgcttt cgctcatctg
     4921 gttcgtaatc tggcggattc accaattccg ctgccgctga tcagtcagta tctgccgctg
     4981 cagtacgtga aagttcgctc cggcgagctg cagccaacgc cacgggaact cattatcaac
     5041 catattcagg acatcctggc gcagtaccac acagcctgtg aaggccaata agcaaaacaa
     5101 agaggaacac gctatgccaa atattgtttt aagtcggatt gatgaacgct tgattcacgg
     5161 tcaggtcggc gttcagtggg tcggatttgc gggggcaaat ctggtgctgg tagccaacga
     5221 tgaggttgcc gaagatctgg tacaacaaaa cctgatggaa atggtactgg cagaagggat
     5281 cgccgtacgt ttctggacgc tgcaaaaagt catcgacaac attcatcgcg ccgccgatcg
     5341 acagaaaatc ctgctggttt gtaaaacacc ggccgatttc ctgacgctgg tgaaaggtgg
     5401 cgttccggtg aatcgcatta acgttggcaa tatgcactac gccaatggca agcaacaaat
     5461 cgccaaaacg gtttctgtgg atgcgggtga tatcgcagca tttaacgacc tgaaagccgc
     5521 tggggtggaa tgcttcgttc agggcgtccc gacagagcct gcagtggacc tctttaaatt
     5581 actttgaggg attcatcatg gaaatcagtc tgttgcaggc atttgcgttg ggaattatcg
     5641 cctttatcgc tggcctggat atgtttaacg gcctaaccca tatgcaccgc ccggtggtcc
     5701 tcggcccgtt ggtcggactg gtacttggcg atctgcatac cggtatttta accggcggta
     5761 cgctggaact ggtgtggatg gggctggccc cgctggcagg cgcacaaccg cctaacgtga
     5821 ttatcggtac tatcgtcggc acggcgtttg ccattactac tggcgtgaaa cccgatgtcg
     5881 cggtcggtgt cgccgtacct ttcgctgtcg cagtacagat ggggattacc ttcctgttct
     5941 cggtgatgtc tggcgtgatg tcccgctgcg accggatggc ggagaacgcc gatacacgcg
     6001 gcattgaacg cgtgaactat ctggcattgc tggcactcgg cactttctat tttctctgcg
     6061 ctttcctgcc tatctacttc ggcgcagaac atgccaaaac catcattgat gtcctgccgc
     6121 aacgattaat cgacggcctc ggcgtcgcgg gcggcatcat gccagcaatc ggttttgccg
     6181 tgctgctgaa aatcatgatg aaaaacgtct acatccccta cttcatcctg ggctttgttg
     6241 ccgcagcctg gctcaagtta ccggtgctgg ctattgctgc cgccgcgctg gcgatggcgc
     6301 tgatcgacct gctgcgtaaa tctcctgaac cgactcaacc tgcggcacag aaagaggaat
     6361 ttgaagatgg catctaatca aaccaccctg ccgaacgtca gcgaaaatga agaaacactg
     6421 ctgactggcg tcaatgaaaa cgtgtatgaa gatcagagca ttggcgcgga gctgacgaaa
     6481 aaagatatca accgcgtcgc ctggcgttcc atgctgttac aggcttcttt caactacgaa
     6541 cgtatgcagg cttccggctg gctgtacggc ctgctgccag cactgaaaaa gatccacact
     6601 aataaacgcg acctggcgcg cgccatgaag gggcatatgg gtttcttcaa tacccatccg
     6661 tttctggtga cgtttgttat cggcattatc cttgcgatgg agcgttctaa gcaggacgtt
     6721 aacagtattc agagcaccaa aattgccgtc ggtgcgccgc tcggcgggat tggcgatgcg
     6781 atgttctggc taacgctact gccgatttgt ggcgggatag gtgcaagcct cgcgctacaa
     6841 ggctctattc ttggcgctgt cgtctttatt gtgctgttca acgtggtaca cctggggctg
     6901 cgttttggtc tggcgcatta tgcttaccgc atgggcgtgg cggctattcc actcattaaa
     6961 gcaaatacca aaaaagtcgg ccatgcggca tctatcgttg ggatgacggt aatcggcgcg
     7021 ctggtggcaa cctatgttcg tttaagcacc acgctggaaa tcacagcggg cgacgcagtg
     7081 gttaagttac aggctgatgt tatcgacaaa ctgatgcctg ccttcttacc gctggtctac
     7141 accctgacca tgttctggct ggtacgccgc ggctggagtc cgctgcgcct gattgcagtg
     7201 accgtggttc tcggcatcgt cggtaaattc tgccatttcc tttaaataca atgaggtatt
     7261 agatgttaag tattattttg acagggcatg gcgggtttgc cagcggtatg gaaaaagcaa
     7321 tgaagcaaat tctcggtgag caatcgcagt ttatcgccat cgattttccg gaaacctcaa
     7381 gcaccgcaca gcttacatcg cagcttgaag aggccatcgc ccaactggat tgtgaagacg
     7441 gcattgtttt tctgacggat ttgctgggcg gcacgccgtt tcgcgtagct tcaacgctgg
     7501 cgatgcaaaa accgggctgt gaagtgatca cagggaccaa tttacaactg ctactagaga
     7561 tggtgctgga acgcgaaggg ttaagcggtg aagagtttcg tgtgcaggcg ctggagtgtg
     7621 gacaccgtgg gctaaccagt ctggtggacg aacttggtcg ctgccatgag gaatgtccgg
     7681 tcgaggaagg aatatgacac acgttctgcg cgccagaagg ctgctgactg aagagggatg
     7741 gttcgatgac catcagttgc gtattgctga cggtgtaatc gcagcaatcg aaccgattcc
     7801 agtgggcgtt actgaacgcg atgcggaact gctctgcccc gcttacatcg acacccatgt
     7861 acacggtggt gcgggcgttg atgtaatgga tgacgcgccg gatgcgctcg acaagctggc
     7921 aatgcacaag gcacgcgaag gtgtcggcag ttggttaccg accaccgtaa ccgcgccgct
     7981 taataccatt catgcggcgc tgaaacgtat tgctctacgc tgccagcgtg gcggacctgg
     8041 tgcgcaagtg ctggggagtt atctcgaagg accgtacttc acgccgcaga ataaaggcgc
     8101 gcatccgccg gagctgtttc gcgagcttga aattgccgag ctggatcaat tgattgccgt
     8161 ttctcagcat accttgcgcg tggtagcgct ggcaccagaa aaagaagggg cattgcaggc
     8221 catccgccat cttaaacagc aaaacgtacg ggtgatgctg gggcatagcg cggcgacctg
     8281 gcaacaaacc cgtgccgcgt ttgatgctgg tgccgacggc ctggtgcatt gctataacgg
     8341 gatgacaggt ttacatcacc gcgaaccggg aatggttggc gcgggattaa cagacaagcg
     8401 cgcctggctg gaactgatag ccgatggtca tcatgtacat ccggcggcaa tgtcgctctg
     8461 ttgttgctgt gcgaaagaga ggatcgtact gatcaccgac gcgatgcagg cagccgggat
     8521 gccggatggt cactatacgt tatgtggtga agaagtgcag atgcgcggtg gcgttgtccg
     8581 taccgcgtcc ggtgggctgg cgggcagtac gctgtctgtc gatgcggcag tgcgcaacat
     8641 ggtcgagttg acgggcgtaa cgcctgcgga agccatccat atggcgtcgc tgcatccggc
     8701 gcgaatgctg ggtgttgatg gtgttctggg atcgcttaaa ccgggcaaac gcgccagcgt
     8761 cgttgcgctg gatagcgggc tgcatgtgca acaaatctgg attcagggtc aattagcttc
     8821 gttttgatgg tttgctcctt tattaggcct tcacttcccc cgtaaggcct ttctttttct
     8881 ttcgttttga tctgtgcagc ggtgtcggat gcgacgctaa tgcgtcttat ccgacctacg
     8941 gttggtgagc gtaaggccgg ataaagtatt tacgccgcat ccagcaatcc cttttacttc
     9001 ccttaccttt tctttcaacg atcacagatt tcgttttatt tcctttttct ccattgaact
     9061 ttcagtttct tttctataga ttttaaccaa cgaaagaaat caccaagtga aatgaaacga
     9121 aaggcaagcg aaagcgacaa cgccgacgtc aagttcatca gactaaggat tgagttatgc
     9181 cagaaaatta cacccctgct gctgccgcaa ccggtacatg gactgaagaa gagatccgcc
     9241 atcagcctcg cgcatggatc cgttcactca ccaacattga cgcgctacgt tccgcgctca
     9301 ataacttcct tgaaccgtta ctgcgcaaag agaatctgca aatcatcctg accggagccg
     9361 gaacatcggc atttatcggt gacatcatcg cgccgtggct cgccagccat accggtaaaa
     9421 acttcagcgc cgtaccgacc accgatctgg tcaccaatcc aatggactac ctgaacccag
     9481 ctcatccgct actattgatc tccttcggtc gatccggcaa cagcccggaa agcgtcgccg
     9541 ccgtgaaact ggcaaatcaa tttgtaccag aatgctatca cctgccgatc acctgcaacg
     9601 aagcgggcgc tctttaccaa aacgcgatca acagcgacaa cgcgtttgcc ctgctgatgc
     9661 ccgcagaaac gcacgatcgc ggcttcgcga tgaccagcag cattaccacc atgatggcca
     9721 gctgcctcgc ggttttcgca cctgagatga tcaacagcca aaccttccgc gacgtggcgg
     9781 atcgttgcca ggcgatcctg acctcactgg gcgatttcag cgaaggtgtg tttggttacg
     9841 caccgtggaa acggatcgtt tatctcggca gcggtggctt acagggcgca gcacgcgagt
     9901 cggcgctgaa agtgctggaa ctgacagcgg gtaaactggc ggccttctat gattccccga
     9961 ccggattccg tcatggcccg aaatcgctgg tcgataacga aacgctggtg gtggtgtttg
    10021 tctccagcca cccttacacc cgtcagtatg atcttgatct gctggcagaa ctccgccgcg
    10081 ataaccaggc aatgcgcgta atcgccatcg ccgcggaaag caacgacgtt attgccgccg
    10141 gtccacatat catcctgccg ccgtcccgtc actttatcga cgttgagcag gcattttgct
    10201 tcctgatgta cgcccagaca tttgcactga tgcagtcgct gcacatgggt aatacgccgg
    10261 ataccccatc agccagtggc accgttaacc gcgtggtgca aggcgtaatc attcatccgt
    10321 ggcaggcata agaggatcgc attatgagca ttatctccac caaatatctg ttacaggacg
    10381 cccaggccaa tggctacgcg gtgcctgcct ttaacattca taacgccgag acgatccagg
    10441 cgatcctcga agtgtgcagt gaaatgcgat cgccggtgat cctcgccgga acgccgggga
    10501 cctttaaaca tatcgcgctg gaagagatct acgccctgtg tagtgcctat tccacaacct
    10561 acaacatgcc actggcgctg catctcgacc accacgaatc gctggatgat attcgccgta
    10621 aagtccacgc aggcgtgcgc agcgcgatga tcgacggcag tcatttcccg tttgccgaga
    10681 acgtgaagct ggtgaaatcg gttgtcgact tctgccactc ccaggattgc agcgtggaag
    10741 cagaactggg ccgcctcggc ggtgttgaag atgacatgag cgttgacgcc gaaagtgcat
    10801 tcctgactga tccacaagaa gctaaacgct ttgtcgaact gactggcgtc gacagcctgg
    10861 cggtagcgat tggtacggcg cacggcttat acagcaaaac gccgaagatt gatttccagc
    10921 ggctggcgga aattcgtgaa gtggtggatg ttcctctggt gctgcatggt gccagcgatg
    10981 tcccggatga atttgtccgt cgcactattg aacttggcgt aacaaaagtg aacgttgcca
    11041 cagaattaaa aatagccttc gctggcgcgg ttaaagcctg gtttgcggaa aatccgcagg
    11101 gtaatgatcc tcgttattat atgcgcgtcg gaatggatgc gatgaaagaa gttgtcagaa
    11161 ataaaattaa tgtctgtggt tcagcgaatc gaatttcagc ataatcatta gttttttatg
    11221 atttatccca atgtacttcc cgattaatat cggggagtgc cttaatggta aaggagataa
    11281 ctaaacctta ataaatacat cagtacaata tcgcaacaat aatatattta aaaagattat
    11341 attattcaac tttatggtga ggattacaca atgagcagtc caaatattct cttaacccgt
    11401 attgataacc gtctggttca cggtcaggtt ggcgtgacct ggacatccac catcggtgcg
    11461 aatctgttag tagtcgtgga tgatgttgtc gctaacgatg ctattcaaca gaaattaatg
    11521 ggtattaccg cagaaaccta cggctttggc attcgtttct ttactatcga aaaaaccatt
    11581 aacgtcatcg gcaaagctgc accacatcag aagatcttcc tgatttgccg tacgccacaa
    11641 acggtacgta aattggtaga aggtggtatt gacctgaaag atgtcaacgt cggcaatatg
    11701 catttctcgg aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc
    11761 acggatttac gttttattaa acaacgtggc gtaaatgttt tcattcagga cgtccctggc
    11821 gatcaaaaag aacaaatccc tgactaaatc taaaatagcc ttaatattgg tttgaggtaa
    11881 taaaaatgca tgaaataacc ctactccagg gattatccct ggcggcgtta gttttctttc
    11941 tggggattga tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc
    12001 ttacaggtgc tattctcggt gatattcaga ctggcttaat taccggtggc ctgacagagt
    12061 tggctttcgc cggattaacc cctgcaggtg gtgttcagcc gcccaaccct attatggcgg
    12121 gtctgatgac cactgtcatt gcatggtcta cgggcgttga tgccaaaacg gcaattggtc
    12181 ttggcctgcc gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct
    12241 cattatttat gaccaaagcc gataaatgcg caaaagaggc ggatacggca gcgttttccc
    12301 gacttaactg gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct
    12361 gtacttacct ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggctga
    12421 cccacggctt tgaagtggct ggcggtattc tgcctgctgt tggttttggc ttgctgctgc
    12481 gcgtgatgtt caaagcacaa tatatccctt acctgatcgc cggtttcctg tttgtttgct
    12541 acatccaggt cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg
    12601 agtttttcaa tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg
    12661 aagaagagga ctacagcaat gggatctgaa atcagcaaaa aagatatcac ccgtctgggc
    12721 tttcgttcgt cactgctaca agcaagcttt aactacgaaa ggatgcaggc gggcggtttt
    12781 acctgggcga tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcctaagc
    12841 gcggcgatga aagataacct cgaatttatt aatacccacc ctaatctggt cggattcctg
    12901 atggggttat taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc
    12961 aaagtggcgc tgtttggccc aatcgccggg attggcgatg cgattttctg gtttaccttg
    13021 ttgccaatta tggcggggat ttgctcatcg tttgccagtc aggggaacct gttgggaccg
    13081 attttgtttt tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc
    13141 ggttattcag tcggcgtgaa ggctatcgat aaagtgcgag agaactcgca gatgattgct
    13201 cgttcggcaa ccatcctcgg gatcacagta atcggcgggc tgatcgcttc gtatgtgcat
    13261 attaacgtgg tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcagcaggat
    13321 ttcttcgata aagtcttccc gaatatttta ccgatggcct acaccctgct gatgtattac
    13381 ttcctgcggg tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct
    13441 attgtttgtt ctgcattcgg cattttgtaa atggaacgag gcactgcgtc tggtggtgcc
    13501 tctttactaa aggaatttca tcctgtgtaa acccttcaac aagttgaaaa ctatacggcg
    13561 ttaagtgaac gtgccagcga atatttattg gccgtgatcc gtcgcaaacc ggatgccgtg
    13621 atttgcctgg cgaccggagc cacgccatta ctgacgtatc attatctggt agaacaaatc
    13681 caccaacagc aggttaatgt cagccagctc accttcgtga agctcgacga atgggtggat
    13741 ctgccattaa cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg
    13801 ctggggctgc gtgaagacca gctcatcagc tttcgctctg aagagataaa tgagacagag
    13861 tgcgaacggg taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga
    13921 ttggggaaaa acggtcatct tgggctgaac gaaccgggag aaagcctgca accggcctgc
    13981 catatcagtc aacttgatgc cggaacacag caacatgaga tgttaaaaac cgcgggccgt
    14041 cccgtgactc gtgggatcac cttaggcctg aaggatattc tcaatacccg cgaagttttg
    14101 ctactggtga ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc
    14161 tctaccgcta tcccggcttc gtttttatgg ctgcataacc atttcacatg cgtgattgat
    14221 gaaatgtgtc gtcgttaact cgcctggtaa aggcgttgta gcgctattcc tgctgcaacg
    14281 cctgtaatca ctctccctgc tgctccagtg catacttata cagcgcattt ttcttcacgc
    14341 cgtgaatttc tgctgccagc gccgccgctt ttttcagtgg cagttctgcc tgcaacagcg
    14401 ccagcgtgcg cagggcatcg gcgggtaagt cttcttcctg tgctttatgg ccttcgacaa
    14461 tcagtaccat ttcgcctttg cgacggtttt catcttcttt gacccacgcc agcagctcgc
    14521 caacgggcgc gccgtgaatg gtttcccagg ttttggtcag ctcacgcgcc agaacgacgt
    14581 agcgggattc gcctaatacc gcaacgatat cttccaggct atctaacaga cggtgggtag
    14641 attcataaaa aatcagcgtg cgcggctccg cttcaatggc ttttagcgca tcacggcggc
    14701 cttttgattt ggcaggtaaa aagccttcat aacaaaaacg atcggatggt aaacccgcgg
    14761 cgcttaacgc agtgatagca gcacacggcc cgggtagcgg caccacgcga atccccgctt
    14821 cacggcaggt acgcaccaga tggtagccag gatcgttaat tagcggcgtt ccggcatcgg
    14881 aaaccagcgc aatgttttgc ccctcttgca gcttcgccag cagcgtttcg gctttttgtt
    14941 gttcgttatg gtcgtgcagc gcaaacaacc gggcattaat cccaaaatgt tgcagcaata
    15001 aaccggtgtg acgcgtatcc tcggcggcaa tcagatcaac ggcctgtaat acctccaacg
    15061 cacgctgggt gatatccgcc agattgccga ttggcgtcgg tacaatgtaa agctggccct
    15121 gagaattatc cgccgattgg tgttgtttca ttgtgtcgtc cgtattgccg atttaatatt
    15181 gagcattgcg taaaaaaaat atcactggat acattatggt accctcaaca ttttctcgtt
    15241 tgaaagccgc gcgttgtctg cctgttgttc tggcagccct gattttcgcc ggttgtggca
    15301 cccatactcc cgatcagtcc actgcttata tgcagggcac ggcgcaggct gattctgcct
    15361 tttacctgca acagatgcag caaagttctg atgataccag gatcaactgg caattactcg
    15421 ccattcgtgc actggtgaaa gaaggtaaaa ccggacaggc ggtggagttg tttaaccaac
    15481 taccgcaaga actgaacgat tctcagcgtc gcgagaaaac actgctggcg gcagagatta
    15541 aactagcgca gaaagatttt gctggcgcgc aaaacttgct ggcgaaaatc acacctgcag
    15601 atttagaaca aaaccagcaa gcgcgttact ggcaggcaaa aatcgatgcc agccaggggc
    15661 gtccttccat tgatttactg cgcgcgttaa ttgctcagga accgctgctc ggcgcgaaag
    15721 aaaagcagca gaatattgac gccacctggc aggcgctctc ctccatgact caggaacagg
    15781 cgaatacgct ggtgatcaat gccgacgaaa atattctgca aggctggctg gatctgcagc
    15841 gcatctggtt tgataaccgt aacgatcctg acatgatgaa agctgggatc gccgactggc
    15901 agaaacgtta tccgaacaac ccgggcgcga aaatgctgcc aacgcagttg gttaacgtaa
    15961 aagcgtttaa accagcatcg accaacaaaa tcgccctgct gttgccgctg aatggccagg
    16021 cagcggtatt tggtcgcact attcagcaag gctttgaagc ggcgaaaaat atcggcactc
    16081 agccagtggt ggcccaggta gcagccgcac ctgccgcaga cgtagcagaa caacctcagc
    16141 cgcaaaccgt ggatggcgtt gccagcccgg cacaagcttc ggttagcgat ctgaccggtg
    16201 aacagcctgc ggcccagtcg gtgcctgtaa gcgccccggc gacaagcacc gcagcggtaa
    16261 gcgcacccgc aaatccatcc gcagagctga aaatctacga tacctcatca caaccactta
    16321 gccagatctt aagccaggtt cagcaggatg gtgcgagtat tgtggtcggt ccgttgctga
    16381 aaaataacgt tgaagagttg ctgaagagca acaccccgct gaacgtgctg gcactgaacc
    16441 agccggagaa catcgaaaac cgcgtcaata tttgttactt cgcgctttca ccggaagacg
    16501 aagcgcgcga tgcagcgcgt catattcgtg accagggtaa acaagcgccg ctggtgctga
    16561 tcccacgcag ttcattgggc gatcgcgtag ccaatgcgtt tgcgcaagag tggcagaaac
    16621 tgggcggcgg ccccgttctg caacaaaaat ttggttccac cagcgaatta cgcgcgggtg
    16681 ttaacggcgg ttcgggtatc gctttaacgg gtagcccgat tactcccaca gaaacaaccg
    16741 actccggcat gacgaccaac aatccaacgc tgcaaaccac gccaaccgat gatcagttca
    16801 ccaataatgg cggtcgtgtc gatgcggtgt acattgtggc aacgccgggt gaaatcgctt
    16861 ttatcaaacc aatgatcgcc atgcgtaacg gtagccagag cggtgcaacg ctgtacgcca
    16921 gctcccgcag tgcgcaaggg accgctggcc cggatttccg cctggagatg gaaggcttgc
    16981 agtacagcga aatcccgatg ctggcaggcg gtaatctgcc gttaatgcag caggcgctca
    17041 gcgcggtgaa caacgattat tcgctggctc gcatgtatgc gatgggcgtc gatgcctggt
    17101 cgttggcaaa tcatttctca caaatgcgcc aggttcaggg ttttgaaatc aacggtaata
    17161 ccggaagcct gacggcaaac ccggattgcg tgattaacag gaagttatca tggctacagt
    17221 accaacaagg tcaggtagtc cccgccagtt aaccaccaaa cagaccggcg atgcgtggga
    17281 agcacaagcg cgtcgctggc tggaaggcaa aggactacgg tttatcgccg ctaacgtgaa
    17341 cgagcgtggc ggcgagatcg atctgataat gcgtgaaggc cggaccacca tttttatcga
    17401 ggtgcgttac cgccgctctg cgctttatgg cggctcggca gccagtgtga cccgcagcaa
    17461 acaacacaaa ttattacaga ctgcccgctt gtggctcgcg cgtcataatg ggagttttga
    17521 tactgtggat tgccggttcg atgtggtagc cttcaccggg aatgaggttg agtggattaa
    17581 ggatgccttt aatgaccact cataattaag gtttaaggat tagcgtgcaa gaaagaatta
    17641 aagcttgctt cactgaaagc attcaaactc aaattgcggc ggcagaggcg cttccggatg
    17701 ccatctcccg tgcagccatg acgctggttc agtctctgct caatggcaac aaaatcctct
    17761 gttgtggtaa tggaacttcc gctgccaatg cacagcattt tgctgccagc atgatcaacc
    17821 gtttcgaaac ggagcggccc agcttacctg ccattgcact aaatactgat aatgttgtct
    17881 taacggcaat tgccaacgat cgcttacatg atgaagtgta tgcaaaacag gtgcgggcgc
    17941 tgggtcatgc gggagatgta ttgttagcca tttccacccg tggcaacagc cgcgatattg
    18001 taaaagcggt agaagctgcc gttacgcgtg atatgaccat tgtggcatta accggctatg
    18061 acggcggtga acttgcaggt ttgttagggc cacaggatgt ggagatccgc attccttcgc
    18121 atcgtagtgc tcgcattcag gaaatgcata tgctgacggt aaattgcctg tgcgatctga
    18181 tcgataacac gcttttccct caccaggatg tttaaggaga atacatgaag gcattatcgc
    18241 caatcgcagt ccttatttcc gcgctgctgt tgcaaggttg tgttgccgct gccgtagtgg
    18301 gtactgctgc tgtgggtacc aaagccgcaa ctgacccacg cagtgtcggc acccaggtag
    18361 acgatggtac cctggaagtg cgcgtgaaca gcgcattgtc gaaagacgaa caaattaaga
    18421 aagaagcgcg cattaatgta acggcctatc agggcaaagt gctgctggtt gggcagtcac
    18481 caaacgctga actttcggct cgcgccaaac agattgctat gggcgtagac ggtgccaacg
    18541 aagtgtataa cgagattcgt cagggccagc cgattggtct gggcgaagcg tctaacgata
    18601 cgtggatcac caccaaagta cgttcgcagc tgttaaccag cgacctggtg aaatcgtcca
    18661 acgtgaaagt gaccaccgaa aacggtgaag tgttcctgat ggggctggtg actgaacgtg
    18721 aagcgaaagc ggcggcagat attgccagcc gggtaagcgg cgtgaagcgg gtaactacgg
    18781 cgtttacgtt tattaaatag cagcccttgt aatgcctgat gcgacgctta cagcgtctta
    18841 tcaggcctac aacgatgcaa accgtaggtc ggataaggcg tttacgccgc atccgacatg
    18901 ttttccctca aatcaaacca acagcgccag cccgccgaca atcacaccag acactgccac
    18961 catcgccccc gtcagccata aggctttcgc cggaaacgct ttacgcaaca ttatcagcga
    19021 cggcaaactc accgccggga gcgtcatcaa caatgccagc gccggagcgg ttcccatacc
    19081 tgccagcatc atcgtttgta caatcggaat ttctgctgcc gtgggaatga caaacaagca
    19141 tcccgctacc gccatcgcca ccacccacat caggctgtta tcgacagcac catcggcatg
    19201 ggggaataac cagacgcgag cggcacccaa caccagtact gcaaggatgt aaaccgggat
    19261 cgtgctccag aaaagcgtcc atagcgccct gccccagcgg ctaaaaaaac cgccctgtga
    19321 ttccggtatg tcaatttcga ccggtgcctg cgtttgcggt gtttcacgca cccatttttg
    19381 caccagcgtc gcaatcagca acaccatcac cagccccgcc accagacgaa tcgccgcaaa
    19441 accccagccg aggacgaacc ccataaacac cagcgtcgcc gggtttaaca acggattccc
    19501 catccagaat gccagcgcac cgcccatcga cacctgttgg cgacgcattc ccgccgcgac
    19561 cggagccgca cagcaggtac acatcatgcc cggcagcgaa aacagcgttc ccagcagcgt
    19621 gccgcgaaag cgcgattgcc caagcgtacg caacaaccag tcacgcggga tcagcacctg
    19681 aatcaacgaa ccgaggatca cccccagcac cgccgctttc cataccgcga gaaaatagat
    19741 catcgcgtaa tccaacgccg cctgccacgg attagcatcc gcctgcgcaa ggatagattt
    19801 accgatactg tgggtttcgg cagcagtaaa cgctttgccg tagtaaggtt gccatttcac
    19861 ataccagaga ccggcaatga caacgagaaa gaaaagcgcg ggtttccacc actgaatggg
    19921 cgttgccgcc tgagatgaag actgaccagt catagcattc cccaggaata agttatgata
    19981 attgagcgcg tgaatattac gctcactatc aattcttggg aataattatt cagctctttt
    20041 gcgtaattct gaagagctta aaatcgtcac accttcatgc tcaggccgca tcgcctctgc
    20101 cagcatgacc cgcgccacgt ctctcgcatc aatggatttc cagttaccgg gtaacaaacg
    20161 gaacagcggc gcaaaaagcg tttcgttcat ccgctgtttg ctacgatcgc ccagtaacat
    20221 cgacgggcga gcaatggtca atttcggcca gttctgggcg attaatgctt cttccatctc
    20281 ccctttgacg cggttataga aaaacggcga gtgggcatta gcacccatag cactgaccac
    20341 cagcatatgc tgcgcaccga gccgccgccc ggttaatgcg gtatccacta ccaacgtgta
    20401 atcggcatga ataaacgcct ctttgctccc cgcttcccgc cgcgtggtgc cgagacaaca
    20461 aaatacaatg tcgatgggat cggtgacctg cgccagcgcg tcggtcagtt gcgggtcatg
    20521 gggattaaac acccctggca tatcgcccaa cgggcgtcgc gtcggcgcgg ctatggcgtt
    20581 aactttcggt tcgttaatca acatccgcag caggtgaccg cccaccagcc ccgttgcgcc
    20641 tgtaatcagt acctgactca tcttcgctcc tttacagaat tgtctgcctt gcgctccccg
    20701 gctcatgcac caggcttaat agtctacgag gtaattatcc ccgatcgtga gaaattcgcc
    20761 tcatccaatg caacaacgcg gaggaaacat gagtaagaag attgccgttt taatcactga
    20821 tgaatttgag gattcagaat ttacttcacc cgcagacgag ttccgtaaag ccggacacga
    20881 agtgattacc attgaaaaac aagcgggtaa aacggtgaaa ggcaaaaaag gagaagccag
    20941 cgtgaccatc gataaatcca tcgatgaagt gacgccagcg gagtttgatg ccctgctgct
    21001 accgggcggt cattcaccag attatctgcg tggtgacaac cgttttgtca ccttcacacg
    21061 cgattttgtg aacagcggca aacctgtttt cgccatctgt cacggtcccc agttgctgat
    21121 cagcgccgat gtgatccgtg gtcgtaagct gaccgccgtt aaaccgatcg tgattgatgt
    21181 taaaaacgcg ggcgcggaat tttacgatca ggaagtggtg gtcgataaag atcagctggt
    21241 taccagccgg acaccggacg atctgccagc gtttaaccgc gaggcgttac gcctgctcgg
    21301 tgcctgagtt acgtagccaa atcatttttt taccaaagcc cagcgtgttg tcggtgaatt
    21361 tgatttcatc gagccggatt tcccataccg gggccgacag cattctggca accggaaagc
    21421 gacgattgta cgccttgcgt gcgaggtcgc tttcctcgcc ttccagtctg cggatctcac
    21481 ctttaaactg cacgccgcga attaacgcta ccgttttcgg ctgaccgttt accgttccgg
    21541 caattgccgc ctgcggcccg ctcatctgcg catgacgcgt tttttcttcc gtcaaaatgt
    21601 agaaagcgac tttctgcgca tcaaaaagat aaaaggcatt ggcgcaccac agttcccctt
    21661 cctgctgcac acaccaggtg acaacatgtt gttttgccag ccaacggctg atggcggtga
    21721 gtgtttccat tgctgttctc ttttatactg tgggcctgac cttaacatgc gacgcagacg
    21781 atgacaccct ggtttcttta cttgatccgt accgccgaca ataagcttta taccgggatc
    21841 accacggatg tcgaacgccg ctatcagcag caccaaagcg gcaaaggggc gaaagcactg
    21901 cgcgggaagg gagaactaac gctggcgttt tccgcaccag tgggcgatcg ttcgctggca
    21961 ttacgggcag aatatcgcgt taagcaactg accaaacggc aaaaagagcg tctggtagcg
    22021 gaaggtgcag tgtttgcaga actgttgaac agcctgcaaa ccccggagat taaaagcgat
    22081 tgaagtgctc gtgatactca accagaccag ttacaccgtt cagcgcgtca tccgctaaac
    22141 gatgtacctg gaaggcactt tcagtgcccg gccagcggca acgcagatcg tgatgtgccg
    22201 ccagttcaaa gccgaaacgg ctgtacagcg ccggatcgcc cagcgtcacc actgcggcat
    22261 agccgaactc attaagcgaa tcgagtcctt catagaccag ttggcgtgcc agcccctgtc
    22321 cacggtattt ttcatctacc gccaacggtg ccatgccgac ccattgcagg tcttcgccct
    22381 gcacatcaac cggactaaat gccacatagc caatgacctg accttcgtca tctgtcgcca
    22441 ccagccccag cgtcagaaag ccatcttcac gcagatcgtg aaccagcttc gcttccgcat
    22501 cactttcgaa tgaacgacgc agcagggcat caatacccgg cgcatcaatg ggaatttcta
    22561 ctcgaattag catggttcac ctaccgatgt ctgtttggtt tcaggcgcgg ttttcattcc
    22621 cgcctcaaca aaatccgcca gttgcagcag catcatgcgt aaggctttcg gcatttgctc
    22681 cagttcaatg gcgtccatca ggtttttcac atacagcccc agctccgtat cgccttcaat
    22741 caccagccga cgctggaaga agagcgtatc cggatcttgt ttacgcgccg caatcatcag
    22801 cagatcgctg gcgtcggcac taaaactcac atcagcttgc gcgttctggc taacgaccag
    22861 tttgccattc accaccgagg taaaccattg caggtcaata tcacgcacat gaatacttaa
    22921 ccagcgacct tcaagaaact ccagctcgcc atcatccagc gcctgacgga attgccagct
    22981 taagacctgc tcaagaacct ggcgttttag cgcaaatggc gtcagtttta ccggtacact
    23041 caacagagat ggccccaaat gcacaatacg ggaacgcagt ttatccaaca cgagttttac
    23101 tccctgtttc aacaatcatc ctattttgcc atatcagaaa aataacatag cggtataaat
    23161 caacaattcc atatgaaatt gctgctacca ccaatacaac tttaactgcc ttaaatcaaa
    23221 aattgtcgca gcaaggttaa ctaaaatccc agttcgttaa catttttgcg ctttgatagc
    23281 gcaaccttca ggaaaaatta tggagctgct ctgccctgcc ggaaatctcc cggcgcttaa
    23341 ggcggccatc gaaaacggcg cagatgctgt ttatatcggg ctaaaagatg ataccaatgc
    23401 ccgtcacttc gccggcctta actttaccga gaaaaaattg caggaagcgg tgagttttgt
    23461 ccatcaacat cgccgcaaac ttcacatcgc gattaacact tttgcgcatc cggacggtta
    23521 cgcccgctgg cagcgcgccg tggatatggc ggcgcagctg ggtgccgacg cgctgatcct
    23581 cgccgacctc gccatgctgg agtacgccgc cgagcgttac ccgcatattg agcgtcatgt
    23641 gtcagtgcag gcttcggcga ccaatgaaga ggcgattaac ttttatcatc gccattttga
    23701 cgttgcccgc gtggtgctgc cgcgcgtgtt gtcgattcat caggtgaaac aactggcacg
    23761 ggtcacacct gtaccactgg aagtctttgc tttcggcagc ctgtgcatta tgtcggaagg
    23821 tcgttgctat ctgtcgtcgt atttgacggg tgagtctccc aacaccgtgg gcgcgtgttc
    23881 tccggcccgt ttcgtgcgct ggcagcaaac gccgcagggg ctggaatccc gcctgaacga
    23941 agtgctgatc gaccgttatc aggacggcga aaacgcaggt tatccgacgc tgtgtaaagg
    24001 gcgttatctg gtggacggcg agcgctatca cgcgctggaa gaacccacca gtctcaatac
    24061 cctggaactg ctgccggagt taatggcagc gaatattgct tcggtgaaaa ttgaaggccg
    24121 ccagcgtagc ccggcgtatg tcagccaggt ggcgaaagtc tggcgtcagg ctatcgaccg
    24181 ttgtaaggcc gatccgcaaa acttcatacc gcaaagcgcg tggatggaga cgctcgggtc
    24241 gatgtccgaa ggcacgcaga ccacccttgg cgcgtatcac cgtaaatggc agtgagaaaa
    24301 gcaatgaaat attccttagg gccagtgctg tggtactggc caaaagagac gctggaggaa
    24361 ttttatcagc aggccgccgc cagcagcgcc gacgtgattt atcttggtga agcggtatgc
    24421 agcaagcgcc gggcaaccaa agttggcgac tggctggaga tggcaaaatc actcgccgga
    24481 agtggtaagc agattgtgct ctccacgctg gcgctggtgc aggcatcctc tgaactgggc
    24541 gaactgaaac gctatgttga gaacggtgag tttctgattg aagccagcga tctcggcgtg
    24601 gtgaatatgt gcgctgaacg caaactgccg ttcgtcgccg gacacgcgct aaactgctac
    24661 aacgcggtga cgctgaaaat attgctcaaa cagggcatga tgcgctggtg tatgccggtg
    24721 gagctttccc gcgactggct ggtgaatcta cttaatcagt gcgatgaact gggtattcgc
    24781 aaccagtttg aagtggaagt cttgagctac ggtcatctgc cgctggccta ctccgcccgc
    24841 tgctttaccg cgcgttcgga agaccgcccg aaagatgagt gtgaaacctg ttgcattaag
    24901 tatccgaacg ggcgcaacgt gctgtcgcag gagaatcagc aagtatttgt actcaacggc
    24961 attcagacca tgagcggcta cgtgtacaac ctcggtaacg agctggcatc catgcagggg
    25021 ctggttgatg tggttcgtct gtcaccgcag gatactgaca ctttcgcgat gctcgacgcc
    25081 ttccgcgcca atgaaaatgg cgctgcgcca ctgccgttga cggcaaacag cgattgcaac
    25141 ggctactggc ggcggctggc cgggctggag ctgcaagcat aagtatatag ctcactttgt
    25201 taacaacttt aactactctt taatgcagta ttaaagatta atcggtaaca aagtgagctg
    25261 ttatgactga taaaaccatt ccgttttcgc tactcgatct ggcccctatt cccgaaggtt
    25321 cttcagcgcg agaagcgttc tcccactctc tcgacctcgc ccgtctggct gaaaagcgcg
    25381 gctatcatcg ctactggctg gctgaacacc acaatatgac cggcattgcc agtgctgcaa
    25441 catcagtttt gattggctat ctggcggcga ataccactac gctgcatctg gggtctggcg
    25501 gtgtgatgtt gcctaatcac tcaccattgg tgatcgccga acagttcggc acgcttaata
    25561 cgctctatcc ggggcgaatc gatttggggc tgggtcgtgc tccgggtagt gaccagcgaa
    25621 ccatgatggc tctacgtcgt catatgagcg gcgatattga taattttccc cgcgatgtcg
    25681 cggagctggt ggactggttt gacgcccgcg atcccaatcc gcatgtgcgc ccggtaccag
    25741 gctatggcga gaaaatcccc gtgtggttgt taggctccag cctttacagc gcgcaactgg
    25801 cggcgcagct tggtctgccg tttgcgtttg cctcacactt cgcgccggat atgttgttcc
    25861 aggcgctgca tctttatcgc agcaacttca agccgtcggc acgactggaa aaaccatacg
    25921 cgatggtgtg catcaatatt atcgccgccg acagcaaccg cgacgctgaa tttctgttta
    25981 cctcgatgca gcaagccttt gtgaagctgc gccgtggcga aaccgggcaa ctgccgccgc
    26041 cgattcaaaa tatggatcag ttctggtcac cgtccgagca gtatggcgtg cagcaggcgc
    26101 tgagtatgtc gctggtgggt gataaagcga aagtgcgtca tggtttgcag tcgatcctgc
    26161 gcgaaaccga cgccgatgag attatggtta acgggcagat tttcgaccac caggcacgcc
    26221 tgcattcgtt tgagctggcg atggatgtga aggaagagtt gttggggtag tgtgttttaa
    26281 cgtaggaagc cttatccgag atggcaacgc tgtcctacgt aggcctgata agcgaagcgc
    26341 atcaggcatt gtgtaggcag cagaaatgtc ggataaggca ccgctgatta ctgatacacc
    26401 ggcagcacat taaagctcga taagatatgg accagtgcgt tgccaacgcc aaataccaga
    26461 atcagcataa tcatcggctt gccgccccat acacggaatt tcgggctacc aaagcgttta
    26521 cgcgatgcgc gggccaacag cgccggaaca attgccgccc agatggtagc cgctaagcca
    26581 gcataaccaa tggcgtacag gaatccgtta ggaaacagca ggccacccac aacaggcggg
    26641 gcaaaggtca gcaacgctgt tttcaaacgg cccatagccg agtcgtcaaa accaaacaga
    26701 tctgccagat agtcaaacaa acccagcgtt acgccaagga acgaactcgc taccgcaaag
    26761 tttgagaaca cgaccagtag aaggtccaga ctacggctgt tcagtacgcc gcttaacgcc
    26821 tgtaccagca catcaatatt accgcccttc tcggcgatgc cgataaactc cggacgcggg
    26881 atgttaccca tcgtcgccag caaccagatg gtatacagcg ccagcgccat cagcgtaccg
    26941 tacaccaggc atttcacgat ggttttcgga tctttgccgt aatacttcat caggctcggt
    27001 acgttaccgt gataaccaaa cgatgccaga cagaacggca gcgtcatcaa cagatacggt
    27061 gcataagacg cattgctttc ggcgacgttg aacaatgtcg caggctgcac atgccccagc
    27121 aggctaccga aggtgaggaa gaaggtaatg actttcgccc ccagcacaat cgctgtcatg
    27181 cgactgacgg ctttggtgct caaccacacc acaaacgcta ccaacagtgc aaaaccaaaa
    27241 cccgctgccc gtgccgggac gtttagcgac atctctgcga aggtgtgatg cagaatcgaa
    27301 ccgctggcag aaatataggc gtaagtcagg atatagagca caaaggcaat ggaaatgccg
    27361 ttgactacgt tccagccttt gcccagcaaa tctttggtga tggtgtcaaa gctagaaccg
    27421 attcgataat tcaggttagc ttccagaatc atcaagccgg aatgcagcat acagaaccag
    27481 gtaaagatca gcgccgccat tgaccagaag aaccacgccc cagacatgac cacaggcaga
    27541 gaaaacatcc ctgcgccaat aatggtgccg ccgataatca ccacgccgcc aagcagcgac
    27601 ggtgacgttt gggtggtggt tagtgttgcc atgagggctt ctctccagtg aaaaatagtg
    27661 cgactgtgtt gttatgcatt gcactgtacc agtacatgag tacaaaaaac agaaaaaaag
    27721 ccccgatggt taaaatcggg gctgtatata ttattttaca gattgtgttc gctgttcagc
    27781 gatgattacg catcaccacc gaaacgacga cgaccggtag agtcatcacg acgcggtgcg
    27841 cggccttcac gacgttcgcc gctaaaacga cgcccatcac cacgaccccc ttcacggcgt
    27901 tcaccgctga agttacgacc gccttcacga cgttcgccac cgaaaccacg accaccgcca
    27961 cgacgctcac cgccagtatg cggctgtgca tcgcccagta actgcatgtt catcggcttg
    28021 ttgagaatgc gagtacgcgt aaagtgttgc agcacttcac ccggcatacc tttcggcagt
    28081 tcgatggtgg agtgagaagc aaacagcttg atgttaccga tgtaacggct gctgatgtcg
    28141 ccttcgttag caatcgcacc aacgatatga cgaacttcaa caccatcatc gcggcccact
    28201 tcaatacggt acaactgcat atcgccaaca tcacgacgtt cgcgacgcgg acgatcttca
    28261 cggtcgccac gcgggccacg gtcgttacga tcgcgcggac cacggtcatc acggtcacgg
    28321 aattcacgtt tcggacgcat cggcgcatct ggcggaacaa tcagagtacg ttcaccctgt
    28381 gccattttca gtagtgcggc tgccagagtt tcgagatcca gctcttcacc ttcggcagtc
    28441 ggctgaattt tgctcagcag tgcacggtat tgatccagat cgctgctttc cagctgctgc
    28501 tgtactttag cggcaaattt ttccagacga cgtttgccca gcagttctgc gttcggcagt
    28561 tctacttccg gaatggtcag cttcatggtg cgttcaatgt tacgcagcag acgacgctca
    28621 cggttctcaa cgaacagcag tgcacgacca gcacgacctg cacgaccggt acgaccgata
    28681 cggtgaacgt aagactcaga atccatcggg atatcgtagt taaccaccag gctgatacgc
    28741 tcaacgtcca ggccacgggc tgcaacgtcg gtcgcaatca ggatgtccag acgaccatct
    28801 ttcaggcgtt ccagtgtctg ctcacgcagc gcctggttca tgtcaccgtt cagcgcggcg
    28861 ctgttgtagc cgttacgctc aagagcttct gccacttcca gagtcgcgtt tttggtacga
    28921 acgaagataa tcgccgcatc aaaatcttcc gcttccagga aacgtaccag tgcttcgttt
    28981 ttacgcatac cccagacagt ccagtagctc tggctgatgt caggacgggt agtcacgctg
    29041 gactgaatgc gcacttcctg cggctctttc ataaagcggc gggtaatgcg acgaatcgct
    29101 tccggcatgg ttgcagagaa cagagcggtc tgatgacctt ccgggatctg cgccataatg
    29161 gtttcaacgt cttcgatgaa gcccatgcgc agcatttcgt cagcttcatc cagaaccaga
    29221 ccgctcagtt tagagaggtc cagagtgtca cgtttcaggt ggtccagcag acggcccgga
    29281 gtaccgacaa cgatctgcgg cccctgacgc agggcgcgta attgcacgtc ataacgttgg
    29341 ccgccgtaca gggcaaccac gtttacgccg cgcatgtgtt tagagaaatc cgtcattgct
    29401 tcagcaacct gtaccgccag ttcgcgggtc ggtgccagca ccagaatctg tggtgctttc
    29461 agctcaggat caagattctg cagcagaggt agagagaatg ctgcagtttt tccgctcccc
    29521 gtctgggcca tacccagaac gtcgcggcca ttcagcagat gtggaataca ctctgcctga
    29581 attggagatg gtttttcgta acccagatcg ttaagggctt caaggatagg agccttcagg
    29641 cccagatctg caaaagtggt ttcgaattca gccatgtagt acgtgtgcct caaaattaat
    29701 ggcggccagt ctacataact catcatgaaa ttgatcagca attttcattg aaaagtgtga
    29761 accggctcaa agtaggtgta ttaacgaaca acaacgccct cacccgttaa ggtgatggca
    29821 atcaaaaaag attacgggct gatgtgtacg tcagctattg ctggtccgat tctgccaggt
    29881 catcttggtc ctggcccagg agcgataatt ccaacaatgc gtatcggtgc tcaacaaagt
    29941 tatgaacgtt gttggcaacc gccagtttga acagtgccgt ggcgctgtcc aaatccccca
    30001 gacttaggta gtacttacct aaatagaagt tggtttcact gagatgctca gcgagcgagg
    30061 tgttatccgt tgcgtccgcc ttgagccttt ccattaacgt ttgttcgcta atgttgccca
    30121 ggtagaactc gacaatgttc catccccact gttccttatc cgatttttcg aagtgctgtt
    30181 tcaacacttc tttagcctgc ttctcatcga gcttctgctc ggcgagataa agccacagac
    30241 tacggaaagg atcattggga tcgtcttgat aaaacgccag cagatcatct tgcgctaact
    30301 tgtcacgacc gccgtaatat aatgcgatcc cgcgattcaa gtgcgcgtag ttgtaagttg
    30361 gatcaagctc aagtacagaa tcaaacgctt cataggcagc atcaaaattg cctgcctgcg
    30421 ttaaatatat gcctaagtaa ttgaatactt caggcatatc cggtcggatt gccagcgctt
    30481 gcgaaaaatc gttacgcgct aatgccctca gaccgagact atcatacaac actccgcgct
    30541 catataaaag ctgtgcgcgt tcgtcatcgg ttaaagcccg actggcaagg atctgttcta
    30601 tacgtgccag aatcacttcc tgctgtaaag tcggttgcaa tggtaccgcg aggacttcac
    30661 ttttacgcca ggaagtatta ctgcatcctg caagcgtaag tgctgtcgca acgaaacacc
    30721 agcgcaaaaa aggcttcatt tcccactccc gaagaccacg gttgaatgaa cgtcctgttc
    30781 ccggttgcta acaaggcgtc ctgcccggtt aaaagccccc cgccgcagcg gagggcaaat
    30841 ggcaacctta ctcgccctgt tcagcagccg gagcttccgg tgctgcagca ggttgagatt
    30901 gctcagtcgc ttctttaatg ctcagacgga tacggccctg gcgatcaact tccagaactt
    30961 tcaccggtac ttcctgaccc atctgcaggt aatcggtcac tttctcaacg cgtttatcag
    31021 cgatttgaga gatgtggacc agaccttctt taccgccacc aatggcaaca aatgcgccaa
    31081 aatcaacgat acgggtcact ttaccattgt agacgcggcc cacttcgatt tctgcagtga
    31141 tctcttcgat acgacgaatc gcgtgtttcg ctttctcgcc gtcggtcgct gcaatcttca
    31201 cagtaccgtc atcttcaatt tcgatggtgg tgccggtttc ttcggtcaga gcacggatta
    31261 cagaaccgcc tttaccgata acatctttga tcttgtccgg gttgatcttg atggtatgga
    31321 tacgcggtgc gaactcagag atatcgccac gcggcgcgtt gatcgcctgt tccattaccc
    31381 ccaggatatg cagacgcgca cctttagcct ggttcagcgc aacctgcatg atctctttgg
    31441 tgataccttc aattttgata tccatctgca atgcagagat accgtcacgg gaacccgcaa
    31501 ctttgaagtc catatcgccc aggtgatctt cgtcgcccaa aatgtcagac agtacaacgt
    31561 agttgtcgcc ttctttcacc agacccattg cgatacccgc aacggcagct ttgattggca
    31621 cacctgcgtc catcagcgcc agagacgcgc cgcacacgga agccatagaa gaggaaccgt
    31681 tggattcggt gatttcagac acaacacgta cggtgtacgg gaatttgtcc atatccggca
    31741 ttactgccag cacgccgcgc ttcgccagac gaccgtgacc aatttcacga cgcttcggag
    31801 aaccgaccat gccggtttcg cctacggagt acggagggaa gttgtagtgg aacaggaagg
    31861 tatcggtacg ttcgcccatc agttcatcaa gaacctgcgc gtcacgagca gtacccagcg
    31921 ttgcggtaac cagcgcctgc gtttcaccac gggtgaacag cgcagaaccg tgagtacgcg
    31981 gcagcacacc agtacgcaca tccagaccac ggatcatatc tttttcacga ccgtcgatac
    32041 gcggttcgcc tgccagtact cggctacgaa caacattttt ctcgatagcg tgcagaattt
    32101 cacccagttc gttttcgtcc agggtttcgt cttcagcaag cagtgtcgcg atggtttcag
    32161 atttgatgac atcaacctgc gcataacgct cttgtttgtc ggtgatgcgg taagcatcgc
    32221 tcagacgagc ttcagccagt gcagcaacgc gcgcgttcag cgcttcgttt accggctccg
    32281 gctgccagtc ccaacgcggt ttaccggctt ctttcaccag ttcattgatg ttctgaataa
    32341 caacctgctg ttgttcatga ccgaacacca ctgcgcccag catttggtct tcgctcagca
    32401 gttcagcttc agattcaacc atcagcacgg cagcttctgt accggcaaca accaggtcca
    32461 gtttgctctc tttcagttcg tcctgagtcg ggttcagtac gtactggtca ttgatgtaac
    32521 ctacacgggc agcaccaatc gggccattga acggaatacc agacagagac agtgctgcgg
    32581 aagcaccaat catcgcgacg atatccgggt taacctgcgg gttaacagaa accacggtgg
    32641 cgataacctg aacttcgttg acgaagcctt ccgggaacag cgggcgaatc gggcggtcaa
    32701 tcagacgcgc gatcagggtt tcgccttcgc ttgggcggcc ttcacgacgg aagaagctac
    32761 ccgggatacg accagcagcg taggtacgct cctgatagtt aacggtcagc gggaagaagt
    32821 cctgacctgg tttggctttt ttctgaccaa caacggtaac aaataccgcg gtgtcatcca
    32881 tgctaaccat aacagcggca gtagcctgac gagccatcat gccggtttcc agagtcacgg
    32941 tgtgttggcc gtactggaat ttacgaacga tcggattaag caatgtaata tcctttctct
    33001 ttcttagaca gtaccttacg gtactggtgt taatacccga tcttctgcgc atcctcgcga
    33061 ctaatgacaa ccctaaccca gctctatgtg ggtaaagcct ctcattagcc gcgcgaacct
    33121 ctgcaacgga agatcattca tagcaacaat acattagttt ccagtgaatt gctgccgtca
    33181 gcttgaaaaa aggggccact caggccccct tttctgaaac tcgcaagaat tagcgacgca
    33241 gacccaggcg ctcgatgagc tgggtgtaac gtgctacgtc tttacgtttc aggtagtcga
    33301 gcagtttacg acgctgagaa accatgcgca gcagaccacg acggctgtgg tgatcttttt
    33361 tgtgctctgc aaagtggccc tgcaggtggt tgatctgtgc agtcagcagt gctacctgaa
    33421 cttcggtaga accggtgtcg tttgcgtcac gaccaaactc agaaacgatt ttagctgttg
    33481 cttcagtact tagagacatt ttaaaactcc aaagtatata gaatgaaagg acgccgatct
    33541 ctaattcagc gatcccagtg tacgttacgc aaagtgttaa acaatttacg cgacgttaag
    33601 cggcagtatt ctactcgtag cgacctgtta tcgcaagacg gttaacatta cgccgggtat
    33661 tcaaccacca ggcgacgagg cgcaacgcgg ccttcatcgt caatttcgcc cataccgata
    33721 aacttgccgt tctcaccctc tgtaacgcga accagtcctt ccagcggcgc accggatgta
    33781 cgaactgggt taccattttt gaagtaaaca gaagacgtta acggaagatt caccactggg
    33841 tagtccgaag ctggactgtc cattggcatc agtaatggat caagtaactc cgcagccgga
    33901 atatcctgct gttcggcttg ctcaaccagt tcacgcagat gttccagggt caccatccgt
    33961 tcaaccggat atttacttac cgccagacga cgcaagtaaa taacatgcgc gccacaaccg
    34021 agtttttcgc cgagatcgtc aatgatagtg cggatataag tgccttttga gcagtgaatt
    34081 tccagctcca gctcattgcc ttcatggcga ataaacagca attcataaac ggtaatcggg
    34141 cgcgcttcac gcggaacttc aatgccctga cgcgcgtatt cgtacagttt tttgccctga
    34201 tatttcagtg ctgaatacat cgaagggatc tgttcgatat cgccacggaa ggtatccagc
    34261 gccgccgcca gttgctctgc actaaaggtt accggacgtt cttcaacgat ttgtccgtcg
    34321 gcatcagaag tatcggtacg ctgtccaaga cgcgcaatga cccgatagcg tttgtcggag
    34381 tccagcagat actgggaaaa cttcgtcgct tccccgaggc aaattggcaa catgccggtc
    34441 gccagcgggt ccagcgcacc ggtatgcccg gcacggttgg cgttataaat ccgtttcact
    34501 ttttgcagcg catcgttgct ggacatcccc tgcggtttat ccagcaacaa aacgccgtta
    34561 atgtcgcgac cgcgacgacg aggacgactc attagtcctc cttgctgtcg tccgggttaa
    34621 cacgacgttc ttcgtcatgt ttgaccacgc tggtcaccag gtttgacatg cgcatccctt
    34681 caaccagaga gttgtcgtag aagaaggtca gttccggcac gatacgcagg cgcatcgctt
    34741 tccccagcag gctgcggatg aaaccagaag cttcctgcaa cgctttgatg cccgctttaa
    34801 ccgcgtcttc atctttgtcg ttgaggaacg tcacatatac tttggcatac gccaggtcgc
    34861 gagacatttc gacaccggaa acggtggtca tcatgcccag acgaggatct ttaatttcac
    34921 gctgcaggat gagagcgatc tctttttgca tttcctgcgc tacgcgctgc gggcgaccaa
    34981 attctttcgc cataataaat tctcctgaca aaaaaggggc tgttagcccc tttttaaaat
    35041 taatttcagg tggaagggct gttcacgttg gcctgataag acgcgccagc gtcgcatcag
    35101 gcaatccatg caggatgcga cataaacgcc ttatcccgca tggaacccta aaaaccttaa
    35161 gcgatagtac gttggatctc gatgatttcg aatacttcga tcacatcgcc agtgcggaca
    35221 tcgttgtagt tcttaacgcc gataccacat tccataccgt tacggacttc gttaacgtca
    35281 tctttgaagc gtcgcaggga ctccagctcg ccttcgtaga taaccacgtt gtcgcgcaga
    35341 acgcggatcg ggttgtgacg tttaaccaca ccttcggtaa ccatacaacc tgcgatagca
    35401 ccaaatttcg gtgatttgaa cacgtcacga acttccgcca gaccgataat ctgctgtttc
    35461 agttccggag acagcatacc gctcatcgcc gctttcactt cgtcgatcag gttatagatg
    35521 acggagtagt aacgcagatc caggctttcc gcttcaatca ctttacgtgc agaggcatca
    35581 gcacgtacgt tgaagccaac caggatggcg ttggacgccg cagccagggt agcgtcggtt
    35641 tcggtgatac cacctacgcc agaaccgatg atcttcactt taacttcgtc agtagacagt
    35701 ttcagcaagg agtcggaaat tgcttccaca gaaccctgta cgtctgcctt caggacaata
    35761 ttcacttcgt gaacttcgcc ttcggtcatg ttggcgaaca tgttctcgag tttagatttc
    35821 tgctgacgcg ccagtttaac ttcgcggaat ttaccctgac gatagagtgc aacttcacgc
    35881 gctttcttct cgtcacgtac aacggtaact tcatcacccg cagccggtac gccggacagg
    35941 ccaaggattt ccaccggaat ggacggaccc gcttccagca cttcctgacc cagttcgtta
    36001 cgcattgcac gaacacgacc gtattcgaag ccacacagaa cgatatcgcc cttgtgcaga
    36061 gtaccttcac gtaccagaac ggtagcaacc ggaccacgac ctttatcgag gaaggattcg
    36121 ataaccgcac cgctcgccat acctttacgt accgctttca gctccagaac ttccgcctgc
    36181 agcaggatag catccagcag ttcatcaata ccggtacccg cttttgcaga tacgtgtacg
    36241 aactggcttt cgccgcccca ctcttctggc aggatgccgt actgggagag ttcgttctta
    36301 acgcgatccg gatcggcttc tggtttatcg attttgttca cagcaaccac caccggaaca
    36361 cccgccgctt tcgcgtgctg gatagcttcg atggtctgcg gcattacacc gtcgtctgca
    36421 gcaacaacca ggactacgat gtccgttgcc tgcgcaccac gtgcacgcat tgaagtaaac
    36481 gcggcgtgcc ccggggtgtc caggaaggtg atcatgccgt tttcagtttc aacgtggtat
    36541 gcaccaatgt gctgggtaat gccgcccgct tcgccagagg ccactttcgt tgaacgaatg
    36601 tagtccagca gagaggtttt accgtggtca acgtgaccca tgatggtcac aaccggcgcg
    36661 cgcggttcag ccgcagcacc cgtgtcacgg tcgctcatta ccgcctcttc cagctcgttt
    36721 tcacgacgca ggataacttt gtggcccatc tcttcagcaa ccagctgtgc ggtttcctga
    36781 tcgataacct ggttgatggt tgccattgcg cccagtttca tcatcgcttt gatgacctga
    36841 gagcctttaa ccgccatctt gttcgccagt tcgccaacgg tgatggtttc gccgatcaca
    36901 acgtcacggt taacggcctg agctggcttc tggaagcctt gctgcagcga agaaccttta
    36961 cgttttccgc ccttaccgcc acgtactgct gcgcgagctt cttcacgatc agcttttgat
    37021 tcagcgtgtt tgttgccctt cttcggacgc gctgctttcg cgttacgacc acggccacgg
    37081 ccgccttcga cttcacgatc gctttcgtct tctgcctggc gagcatgttg agaagtagtg
    37141 acgtgataat cgctggaatc ttcagtcggt tctgcgttat cagtccattt gttttcttcc
    37201 gccatacgac gtgcttcttc agcaacgcga cgtgcttctt cttcgagttt acgacgcgct
    37261 tcttcttcgg ctttacgctt gagctctgca gcttcctgct cacggcgggc tttttcagcc
    37321 tgggcgtttt tagtcatatc gtcttgttga ttgctcactt tgtctttttc cgcagcttca
    37381 cgtttcgctt gttcagcagc ttcacgctta gcttgttctg cggcctcacg ttcagctttt
    37441 tgttgcgcct cacgtttggc cgattcttct gcctcacgac gggcttgctc ttccgcttca
    37501 cgctgcgctt gctcttccgc agcaaggcgt tcagcctctt gcggatcgcg tttcacaaag
    37561 gtgcgtttct tgcggacttc gatttgtacc gatttgcttt ttccaccggt accaggaatg
    37621 ttaagggtgc tgcgtgtttt acgttgcagc gtcaatttgt ccgggcctga atttttctga
    37681 ttcaggtggt caatcaaagt ctgtttctct tgtgcagaca cagagtcgtc agcagacttc
    37741 cggatacctg catcagcaaa ttgctgtacc aggcgttcca cggaggtctg tcgctctgcg
    37801 gccagcgttt taatcgttac atctgtcatg ctgttccttc ctgctacagt ttattacgct
    37861 tcgtcaccga accagcaaat attacgggca gccataatca gtgctccggc tttttcgtcg
    37921 gtcaaccctt cgatatcagc cagatcatca atgccctgtt cggcgagatc ttccagcgta
    37981 caaacgccac gggcggccag tttgaatgcc aaatcacgat ctaccccttc aaggttcagc
    38041 agatcgtcag ccggtttgtt atcaccgagg ctttcttcct gggcctgtgc aatggtggcc
    38101 agtgcatttt tagcacgctc gcgcagtgct tcaacggtcg gctcatcaag gccttcgatt
    38161 tccaacagct ctttcatcgg cacataggcc agttcttcca gcgtcgagaa gccttcttct
    38221 accagaacag tcgcgaagtc ttcatcgata tcgagatatt tggtgaaggt gtcgatcgct
    38281 gcgtgcgctt ccgcctgatg cttagcctgc aggtcgtcaa cggtcatcac gttgagttcc
    38341 caaccgctca gctgcgaagc cagacgcacg ttctgaccgt tacggccaat cgcctgcgcc
    38401 aggttaccgg cttcaacggc gatatccatg gtgtgtttat cttcatccac cacgatagaa
    38461 gcaacgtctg ccggtgccat tgcgttaatc acaaactgcg ccgggttatc atcccacagg
    38521 acaatatcga tacgctcgcc gcccagttca gtagaaaccg cctgaacacg cgcgccacgc
    38581 atacctacgc aagcacctac cggatcgata cgtttatcgt tggttttcac cgcaattttc
    38641 gcacgagatc ccgggtcgcg agccgctgct ttaatttcaa tcacttcttc gccgatttcc
    38701 ggcacttcaa tacggaacag ttcgatcagc atttccggct tggagcgggt gacgaacagc
    38761 tgcgcgccac gcgcttccgg gcgaacggaa tagagcacgc cacgaacgcg gtcgccaggg
    38821 cggaagtttt cacgcggcag catatcttcg cgcaggatca cggcttcagc gttgttgccc
    38881 agatccagag agatgttgtc gcggtttact tttttcacca cgccggtgat gatttcacct
    38941 tcgtgttcac ggaactgatc aaccaccatt gcacgttcgg cttcacgcac tttctgcacg
    39001 ataacctgtt ttgccgtctg ggtagtgata cggtcaaagg taacagactc aatctgatct
    39061 tcaacgtaat cgcccaggtt caggctttca tcttcataac gtgcggcttc aagggtgatt
    39121 tccttggtcg gctgggtgac ttcatcaaca actaaccagc gacggaaggt gtcaaaatca
    39181 ccgcttttgc gatcgatctg tacgcgaacg tcgatttctt gttcatattt tttctttgtt
    39241 gctgtcgcca gcgcgctttc caatgcttcg aaaatcttct cgcgaggtag cgccttttca
    39301 ttggatacgg cttcaactac agccaaaatt tctttgttca tcgcgggctt ttcacctcat
    39361 ccagactatt aaaagtgggg aaccaggttc gccttctgga tattactcag cgcgaacact
    39421 tcatctttac cttcgacggt aactgtgatc atttcaccgt ctaccgcttt gataacgccc
    39481 tgccatttac gacggttttg taccgccata cggagaacca gagtcacctc ttctccgaca
    39541 aaacgggcgt agtgttcagc cgtgaacagt gggcgatcga gacccggtga ggagacttcc
    39601 aggttataag caacggtgat ggggtcttca acatccagca cagcacttac ctggtggctc
    39661 acatcagcac aatcatcaac attgatgcca tcttcactat caatatagat gcgcagtgtg
    39721 gatgtgcgac cgcgaataaa ttcgatgcca accagttcaa aacccagggc ctcaactggc
    39781 gcagtaatca tctctgttaa tttttgctct aatgtggaca agcccacccc caagacataa
    39841 aaaaagggcc taaagcccag ttattctgta gtcagataac aaaaaacccc gataaatcgg
    39901 ggctttatat aactgaaccc tataaccgca actgcggtct ggagcacttt ccagaaggat
    39961 tttttcaaat cccactacga aggccgaagt cttcacagta tatttgaaaa aggactctaa
    40021 gggaaagtgg ttgcgggggc cggatttgaa ccgacgacct tcgggttatg agcccgacga
    40081 gctaccaggc tgctccaccc cgcgcctgaa acgtggcaaa ttctactcgt tttgggtaaa
    40141 aaatgcaaat actgctggga tttggtgtac cgagacggga cgtaaaatct gcaggcatta
    40201 tagtgatcca cgccacattt tgtcaacgtt tattgctaat cacgtgaatg aatatccagt
    40261 tcactttcat ttgttgaata cttttacctt ctcctgcttt tccttaagcg cattatttta
    40321 caaaaaacac actaaactct tcctgtctcc gataaaagat gattaaatga aaactcattt
    40381 attttgcata aaaattcagt gagagcagaa atccaggctc atcatcagtt aattaagcag
    40441 ggtgttattt tatgacgacg attctcaagc atctcccggt aggtcaacgt attggtatcg
    40501 ctttttccgg cggtctggac accagtgccg cactgctgtg gatgcgacaa aagggagctg
    40561 ttccttatgc atatactgca aacctgggcc agccagacga agaggattat gatgcgatcc
    40621 ctcgtcgtgc catggaatac ggcgcggaga acgcacgtct gatcgactgc cgcaaacaac
    40681 tggtggccga aggtattgcc gctattcagt gtggcgcatt tcataacacc actggtggac
    40741 tgacctattt caacacgacg ccgctgggcc gcgccgtgac cggcaccatg ctggttgctg
    40801 ctatgaaaga agatggcgtg aatatctggg gtgacggcag cacctataaa ggaaacgata
    40861 tcgaacgttt ctaccgttac ggtctgctga ccaatgctga actgcagatt tacaaaccgt
    40921 ggcttgatac tgactttatt gatgaactgg gtggccgtca tgagatgtct gaatttatga
    40981 ttgcctgcgg tttcgactac aaaatgtctg tcgaaaaagc ttactccacg gactccaaca
    41041 tgcttggtgc aacgcatgaa gcgaaggatc tggaatacct caactccagc gtcaaaatcg
    41101 tcaacccaat tatgggcgtg aagttttggg atgagagcgt gaaaatcccg gcagaagaag
    41161 tcacagtacg ctttgagcaa ggtcatccgg tggcgctgaa cggtaaaacc tttagcgacg
    41221 acgtagaaat gatgctggaa gctaaccgca tcggcggtcg tcacggcctg ggcatgagcg
    41281 accagattga aaaccgtatc atcgaagcaa aaagccgcgg catttatgaa gcccctggga
    41341 tggcactgct gcacattgcg tatgaacgtt tgttgaccgg tattcacaac gaagacacca
    41401 ttgagcagta tcacgcgcat ggtcgtcagt tgggccgcct gctgtaccag ggacgttggt
    41461 ttgattccca ggcgctgatg ctgcgtgact ctctgcaacg ctgggttgcc agccagatca
    41521 ctggtgaagt tactctggag ctgcgccgcg ggaacgatta ttcaatcctg aataccgtct
    41581 cagagaacct gacctacaag ccagagcgtc tgacgatgga aaaaggcgac tcggtgttct
    41641 cgccagatga tcgtatcggt caactgacca tgcgtaacct ggatatcacc gatacccgcg
    41701 agaaactttt cggttatgcc aaaactggcc tgctctcctc ctctgccgct tcaggggtgc
    41761 cgcaggtgga gaatctggaa aataaaggcc agtaattcga catcaaccct gcccttcagg
    41821 ggtggggtta ccttgattat cctctgtaaa ctcctttctg agcagcacat aatcgaaaac
    41881 atcatggttt gcatccacaa cccgtgagtc tcctttatag tgtgcaatca cttcctcaag
    41941 cgtttgcggc tgttccggct ttgttacccc catccacgca ttaataaggt aattattgta
    42001 agctgtcgaa aagatgttgt tttccgttgt gcgatccacg ccatccccaa gaacggggct
    42061 ataccagata aacatcggga catgatacgc ctgctggcta gcctccctgc ctccatgaaa
    42121 atagacgttc ttcttcgtag ggtcgcgttc caggccgtgg tcggcaaaat acatgaccga
    42181 ggcgcggcaa tcttttaata attcaaaaac ctgacctagc aaactatctg tgtaatgaat
    42241 ggagttgtca tagcaggcat cctgatcgtc ctgcggttga aacacagcgc tggattgcgg
    42301 ataggcgcta caagccggtt catggcttcc atttaaatga agaacaatca gtttcttttg
    42361 ctgcgtcttt tgctgtaacg cttgcgataa atgcggcaac aacaattcat caaatcctct
    42421 gacatagact gtttccatgg cgcgcatggc gatactggta actgctgtac cattctgccg
    42481 aaaagcggat tgcgagctta gccagaaagt ctgaaatcct gcttgattag ccatattaat
    42541 aatgttgtcc aggtaattat gaatgtcatg actcaaaaca gaatcagcag ttaaagaaag
    42601 gggaaccgac agcgcagtgt aaggtgcacc gcttattgct tgattaaaca gtttgatctg
    42661 ctttctttgt gcttcgactt gcggtgtcgt agagcgtgta tatccataca aagacatatt
    42721 gtcgacacgt acagactccc ccacaatcaa cacgtaggta tcaatacctg tatccctgac
    42781 tgatagttga aaatacggca ccgtgtttgc aattgagagt aatctttgat gctctttcgc
    42841 tgctaaagca aaataattga gattgaaaaa cggcgtatag gtagcaaatc gcgacgctag
    42901 tatatatgga ctgaacgcct ctttattttt tgcatcttta taagcaaatt gacaagcgga
    42961 aaataaactg cccgaaatga caatcagcaa taatattcct gtcacttttt ttgtcggcaa
    43021 ggaaacatca tattttatta ttaccgccaa aaaaagaagg gataaaaagg taaaagcaca
    43081 tagataaggg atatacattc ccagcatttt gacaacttca tccggatcac tctgcagcac
    43141 actaatcgca aatccatcat tgaaagttgt accaaagata caccatgaat aaaggctaat
    43201 actcatatct gccgcagtca gtaacacaaa tggtacggca ataagcaggc gaagcggaag
    43261 ggataatata cgtttaacaa ccaggatggt tagaacgaag aacagcaatc gctgaaccat
    43321 atgcgctccg gaagaagcga ctaagttcgt aataccaaaa acaataacac aaagatacaa
    43381 caaccaatga gatttaaaac ttttaacaaa tttgttgaat actgtcatcc ggaaatccaa
    43441 gttcacaacc aatataaaca attatcaaca atgttcaatt ttatatcaga catgtttttc
    43501 aactctgatc catatcagac cgaaacactg ttagtttttt ctttttaaat gatgcggcca
    43561 ttgttctcag taattaaata acattatcaa tgcgtctcta aacattacgc agaacgtagc
    43621 catcagtagt tatactgaca ggagcaggat ggtttatcga gaagtaaaca tatccgtatt
    43681 cgtaccggaa ctggagttga cacaataaag tgccaattat gtcagcagaa gggaaaagtt
    43741 tataactaag gcgtaccggc accatcgttt caaagtacca gctacgagta aagcaactgg
    43801 acgagataca gatacctgac aaccattcct cagaccagga ccaaaacgaa aaaagacgct
    43861 tttcagcgtc tcttttctgg aatttggtac cgaggacggg acttgaaccc gtaagcccta
    43921 ttgggcacta ccacctcaag gtagcgtgtc taccaattcc accacctcgg cacggatact
    43981 actttttagt tcgggatatc gctggtcggc ttagccggag cagctggctg agtttgttcg
    44041 gttttcgccg gcgcactcag gttttcccat tcgctacctt tattggtttt attgctattg
    44101 atgttaccca gcaccagact gatgatgaag aataacgttg ccagcagcgc cgtcatgcgg
    44161 gtcatgaagt taccagaacc acttgaacca aacagcgtag cggaagcgcc tgctccgaag
    44221 gaggctccca tatcagcgcc tttaccttgc tgcagcatga tcagaccaac aaggccagtt
    44281 gccacaataa ggaaaactac taaaagagct tcgtacataa tcaacctgtt ccttgcggag
    44341 ttgccgcgta ccaatgcttc aaccaataaa gcgggagttt ttaatctttc ccactgaagc
    44401 gggtgtgaat actaaccaaa gcgaatgacc ttcgcaaggg caattttatt gcattgtatc
    44461 aactgcggaa aaaaacagca aaaccgatgt gttcgctgag aaaaaaggcg acaatcgccg
    44521 cctttttagc cagttatcta acgctttaaa cggcttttac tgcatcggcg atgcggtgtg
    44581 caaattcagt cacctgcgct tcgtcttcgc cttccaccat cacgcgaatt aacggttcgg
    44641 tgccggattt acgcaacagc acacgtccac ggctacccag cgccgcttca acttctgcgg
    44701 tcacagcttt gactgattca tgctccagcg gatcgccgct acctgcggtg taacgcacgt
    44761 ttaccaggat ttgcgggaac attttcatgc cgctgcaaag gtcgtgcagg ctcatatgat
    44821 tacgcgccat cgccgccagc acctgcaaac cagcaacgat gccgtcaccg gtagtggttt
    44881 tatccagcag gatcacatga ccggaattct ctgcaccgat acgccagcct ttctcctgca
    44941 ttttttccag tacgtagcgg tcacccactt tcgcgcgcgc aaatggaatt cctaactgtt
    45001 tcagcgccag ttcaagcccc atgttgctca tcaatgtacc cacagcgcca ccacgcagct
    45061 ggccctgacg aagaccttca cgcgcgatga tatacatgat ctgatcgcca tcgactttat
    45121 tgccttcatg gtcaaccata atcacgcgat cgccgtcgcc gtcgaaggca ataccgagat
    45181 ccgctttttc agccagcaca cgagcctgga gcgcgcgaac gtcggtagcc cccacttcgg
    45241 cattgatgtt tacaccgttc ggctcacaac cgatagcgat aacgttcgct cccagttcac
    45301 gcagcacgtt cggcgcgatg tgataagtcg caccgtttgc acaatccacc acaatcttca
    45361 gttcactgag gctaagttcg tttgggaacg tggctttgca aaactcgata tagcgacccg
    45421 cagcatcaac gatacggctg gctttaccca gttctgccga atcaacacag ctgatctctt
    45481 tttccatttc cgcttcgatg gcctcttcta ctgcatccgg tagtttggtg ccgtcgatag
    45541 agaaaaattt aatgccatta tcgtagaacg ggttatgcga tgcagatatc acaattccgg
    45601 cctctgcgcg gaaggtacgc gtcagataag ccacggccgg tgttggcatc ggaccagtga
    45661 agagtgcgga aaggcccgca gccgccagac ccgcttccag tgctgactcc agcatgtagc
    45721 cagaaatacg cgtgtcttta ccaatgatga tcttacggga gccgtggcgc gccagcactt
    45781 tacccgcggc ccaacccagc ttaagcacaa aatcaggggt gatcggcgca tcccctacac
    45841 gaccacgaat cccatcggta ccgaaatatt tacgattact catagcgttt gttttccttt
    45901 gcagacagag tggcttccac cacccgcatc gcttctacgg tttctttgac gtcatgaaca
    45961 cgaatgatat gcgcgccttg catagcggca atgaccgcac aagccagact accgctcaga
    46021 cgctcggacg gccccacgtt cagcagctgc ccaatcatcg attttcgtga catacccacc
    46081 aacagcggca ggttgaaatg gtgaaattca gccaggcgcg ccagtaatga atagttatgg
    46141 gagagatttt taccgaaacc gaatccgggg tcgagcaaca atttctcttt tgcgataccc
    46201 gcctgctcgc aacgtgctat ttgctcaata aaatagcgat tcacttctgc aaagacatcg
    46261 tcatacttcg gggcttcctg catggtcttt ggatttccct gcatatgcat cagacaaacc
    46321 ggtaaaccgg tttctgcagc tgcctccaga gcccctggtt cggaaaggga gcggatatcg
    46381 ttaataatgt gagcgccaac tttcgctgac tcacggatga cttctggttt ggatgtatcg
    46441 accgagatcc agacttcgaa gcgttgagca attgcctcaa ccacaggaat aacacgttgc
    46501 aactcttctt caacgctaac ttccgccgct cctgggcgcg tggactcgcc accaacgtca
    46561 atgatcgtcg cgccagcgtt gatcatcaga ttcgcatgtt tcaccgcatc tatcagcgag
    46621 ttatgcgtac cgccatccga aaaggaatca ggcgtgacgt tgaggatccc cattacgtga
    46681 ggatggctaa ggtccagtga agtgccctgg gcaaagagtt tcatggtgtt atccctggta
    46741 tgaattgata agaaaaaacc ccggagcacg ccccggggtt ttcggtacaa atacagtcat
    46801 ctgatgcggg aacttatttg tcgcctaact gctctgacat ggtgttaccc gggttcggcg
    46861 tacgcggttc atcaaccgga cgaggagcct ttggactacc attgtcgcca gaattgttag
    46921 aagcgcctgg ttcttcccag cccgctggcg gacgtacatc gcgacgtgcc atcaggtcat
    46981 caatctgcgg tgcgtcgata gtctcatatt tcatgagagc atctttcatc gcatgcagaa
    47041 tatccatatt gtcggtcaga agctgacgcg cacgattata gttacgctca atcagtgctt
    47101 tcacttcctg gtcgatgata cgtgcagttt catcggacat atgtttcgct ttcgctacgc
    47161 tacggccgag gaacacttca ccttcttctt ccgcgtacag cagtggaccc aatttctcag
    47221 agaagcccca ctgagtcacc atgttacgtg ccaggttggt cgcaacttta atatcgttgg
    47281 acgcaccggt agaaacatgt tccggcccgt agatgatctc ttccgccaga cgaccaccgt
    47341 acagcgtaga aatctggctt tccagtttct gacggctggc gctgattgcg tcgccctcag
    47401 gcaagaagaa agtcacaccc agcgcacgac cgcgtgggat aatcgtcact ttgtgcaccg
    47461 gatcgtgttc cggcaccagg cgaccgataa ttgcgtgacc cgcttcgtgg taagccgtcg
    47521 attctttctg cgcttccgtc atcaccatgg agcgacgttc cgcacccatc atgattttgt
    47581 ctttcgcttt ctcgaactca accatcgaca caacgcgttt gttgccacga gcagcgaaca
    47641 gtgccgcttc gttcaccagg ttcgccaggt cagcaccgga gaaaccagga gtaccacggg
    47701 caatgattgc cgcgtcgata tcgggtgcca atggtacgcg acgcatgtga accttcagga
    47761 tctgctcacg accgcgaaca tctggcaagc caaccacaac ctgacggtcg aaacggccag
    47821 gacgcagcag cgctgggtcg agaacgtccg gacggttagt cgcggcgata acgatgatac
    47881 cttcgttacc ttcgaagcca tccatctcaa ccagcatctg gttcagagtc tgttcacgtt
    47941 catcgtgacc accgcccaga cctgcgccac gctggcggcc tacggcgtcg atttcatcga
    48001 taaagatgat gcacggtgcc gctttcttcg cctgttcgaa catgtcacga acacgggatg
    48061 cacccacacc gacgaacatt tctacgaagt cagaaccgga gatagtaaag aacggaactt
    48121 tcgcttcgcc tgcaatcgct ttcgccagca gcgttttacc ggtacccgga ggaccgacca
    48181 tcaggacgcc tttcgggatc ttaccgccga gtttctggaa gcggctcggc tcgcgcagat
    48241 actcaaccag ttcagcaact tcttcttttg cttcgtcgca gcccgcaacg tcagcaaagg
    48301 tcgttttgat ctgatcttca gtcagcatgc gcgctttgct cttaccaaac gacatggcac
    48361 ctttgccacc gccgccctgc atttgacgca tgaagaagat ccagacacca atcagcaaca
    48421 acatcgggaa ccaggagatg aagatagaag ccagcaggct tggttcttca ggcggttcac
    48481 cgacaacctt gacgttcttg gtcaacaggt tatccagtaa tttcggatcc tgaaccggaa
    48541 tgtaagtggt ataacggtta ctatctttct tggtaacgtt gatttcacgt ccgttgatac
    48601 gcgcttcacg aacctggtcg ttattgacct cttgtaggaa ggtagagtaa tccaccttac
    48661 ggccattaga ctcgctgggc ccaaagctct ggaatactga catcagcaca acggcaatga
    48721 ccagccagag tattaggttt ttcgccatgt cactcaaggg attaacctct tattacaact
    48781 gtgttaaaaa cagcgtcagg atactctata tccagcatct ttcaaacttt cgtctgaaat
    48841 ctcccggtta gggtttacgc ccggtcgcta caatatacac ttcccgcgaa cgtgcacgag
    48901 aagagtccgg cttacgaact ttgaccttcg taaacaggga gcgaatttcc cttagatact
    48961 catcgaaacc ttcgccctgg aacaccttca ctacaaaact gccacctggc gctaatacat
    49021 cacgacacat ttctagcgcc agttccacca gatacatggc acgggggata tccaccgccg
    49081 gtgttccgct catgtttggt gccatatcgg acatgacaac ctggactttg ctgtcgccaa
    49141 cgcgctccag cagtgctttc atcaccagtt catcacgaaa atcgccctga agaaagtcca
    49201 caccaacgat aggatccata ggtaaaagat cgcaagcgat gatgcggcct ttgccgccaa
    49261 tttgggtgac cacatattgt gaccaaccac ccggagcagc accaaggtcg acaaccgtca
    49321 ttcccggttt aaagagtttg tcactttgct gtatttcatc aagtttaaac caggcacggg
    49381 aacgtaaccc ctttttctgt gcctgttgaa catatttatc gctaaagtgt tcctgaagcc
    49441 agcggctgga gctggcagaa cgcttcttac ctgtcattta actttcccat ggggataact
    49501 catcgtaacc aattgcgtaa atttttacgc gcctatttgg tgatatatgg gagatggcgg
    49561 tagaatgacc cgttttcaat cccaacgtaa gcaaaaaata cgatgaatct gagtactaaa
    49621 caaaaacagc acctgaaagg tctggcacat ccgctcaagc cagttgttct gcttggcagt
    49681 aatggtttga ccgaaggggt gctggccgag attgaacaag cgttagagca ccatgaactc
    49741 atcaaggtga aaatcgccac cgaagatcgc gaaactaaaa ccttgatcgt ggaagctatc
    49801 gtgcgcgaaa ccggcgcctg taatgtacag gtcatcggta aaacgctggt gctttatcgc
    49861 ccaactaaag aacgtaaaat ctcgctgcca cgctaagatt atcctaaagt tacacacatt
    49921 cgctgtgtaa aacgaggggt tttccgcagg caggagagca aaatgccacg ctctgttcgt
    49981 tgataaaagg ccgcatagcg gcctttttcc tttctttaca atacatcaac atcttgagta
    50041 ttgggtaatt cttacaggta ttccacctta attacttcaa attctacttc gccgcccggc
    50101 gttttgatga ccacaacatc atcttcttct ttgccgatca ggccacgagc aataggcgag
    50161 tttacagaaa tcaggttttg tttaaagtca gcttcgtcat cgccaacgat gcgataagtc
    50221 tgttcttcgt cagaatcgag attcagcacc gttacggtag caccaaaaat aacgcgccca
    50281 ttgttgggca ttttggtgac atcaatcacc tgcgcgttcg acagcttggc ttcgatgtct
    50341 ttgatacggc cttcgcaaaa gccctgctgt tcacgagctg cgtggtattc ggcgttttct
    50401 ttcaggtcgc catgctcacg cgcttccgcg atagcagcaa tgatttcagg acggcgcaca
    50461 gatttcagaa aatccagctc ttcgcgtaat ttttcagcgc cgcgtaaggt catcggaata
    50521 gcttgcattt gttatacctc ttgaatattc ctgatggggc aagtcttcac ccaccccggc
    50581 tgttcggcct gcccggcata actcttcacc gggaccagaa gcaaaaaata ccgacccggg
    50641 tacaagtccc aggtcagcta caattcacat tttgatagtc attttaccct gaagttcccg
    50701 aagggtcatc gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt
    50761 gttagcgcga gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt
    50821 tcagtgttca ggccgcaaat gttgatgagt acattactca actccccgct ggtgccaacc
    50881 ttgccctgat ggtgcaaaaa gtcggcgcgt cggcccccgc tattgattac cacagtcagc
    50941 agatggcgct gcccgccagt acgcagaaag tgattactgc actggcggcg ttgattcaac
    51001 tcggccccga ttttcgtttt accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg
    51061 tacttaaggg tgacttagtg gcgcgatttg gtgccgatcc gacgttaaga cgtcaggata
    51121 ttcgcaatat ggtcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt
    51181 tgatagatac ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca
    51241 tgacacaatg ctttagcgct ccgcctgccg ccgccatagt tgaccgcaac tgtttctccg
    51301 tctcgctcta cagtgcccca aaacctggtg atatggcttt tatacgcgtg gcatcttatt
    51361 accccgttac gatgttcagc caggtacgca ccctcccccg tggttctgcc gaagcgcaat
    51421 actgcgagct ggatgtggtg ccaggcgacc tgaaccgctt tacgctgacc ggatgcctgc
    51481 cacaacgttc tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg
    51541 gtgcaattct gaaagatgag ttaaaacagg caggtatcac ctggagcgga acactgctgc
    51601 gccagactca ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgctgc
    51661 acgatctgct taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc
    51721 gcatgatagg ccatgcgcgc ttcaatgtgc ctggaacatg gcgggccggg tcggacgccg
    51781 tgcgtcagat cctgcgccag caagccggtg tcgatattgg aaacaccatt attgccgatg
    51841 gttcagggct ttcgcggcat aacctgattg cccccgccac catgatgcag gtgctgcaat
    51901 acattgccca acacgacaat gaacttaact ttatctccat gctgccgctg gcgggctatg
    51961 acggctcttt gcagtaccgt gcaggtctgc atcaggcggg cgtggatgga aaagtctcag
    52021 cgaaaaccgg ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg
    52081 ggcaacgaat ggcgtttgtg caatatcttt ctggctatgc agtagaaccg gcggatcagc
    52141 gtaatcgccg tattccgttg gtacgttttg aaagccgttt gtataaagat atttaccaga
    52201 acaattagtc taaagagaaa ccccggcaca tggccggggt tttgctgatt aacgcttgta
    52261 aatgaactca acgccttctt cgtcgtcttc gtcccagtcg tcatcccagt cttcatcatc
    52321 ctcttcagca atctcttcaa gctgctggcg atgataatca tcccacatga attcgacttt
    52381 ctctggctgt ttcgcttctt cagcctgcac gaccgggttt tcaatgataa aggtcatcac
    52441 atcccagcag agatctttca cgcccagtcc gctcgccgca gagatcagat aatatttatc
    52501 ttcccaaccc agcgcctcag cgatcgcttt cgctttctct tcggcttctg ccttatccag
    52561 caggtcgatc ttgttgaaga ctaaccaacg cggtttcgcc gccagatcct ggctgtattt
    52621 ttccagctcg ctgataataa tacgcgcgtt ttcaaccgga tcggtgccgt caatcggatc
    52681 gatatcgatg aggtgcaaca gaacgcggca acgttccagg tgcttcagga agcgaatgcc
    52741 cagacctgcg ccttctgcag cgccttcaat cagtcctgga atatcggcaa caacgaagct
    52801 cttttcgttg tccattcgta ccacaccaag acttggcacc agagtggtaa acggataatc
    52861 cgccactttc ggtttagccg ccgataccgc acgaataaag gtcgatttac ccgcgtttgg
    52921 catccccaac ataccgacgt cagccagcag catcagctcc agcagcagct cacgcttatc
    52981 gcccggcgta ccgttagttt tctgccgtgg tgtacggtta actgacgatt tgaaacgggt
    53041 attgcccaga ccgtgccagc cgcccttagc aaccagcaga cgctgaccgt gtttggtcat
    53101 atcacccatg gtttcaccag taccctggtc gattacacgc gtacctaccg gcactttaat
    53161 cgtgacgtct ttaccgcgct taccggtaca gtcgcggctt gcaccgtttt gaccgcgctc
    53221 tgcacggaaa gatttttcaa aacgataatc gataagcgtg ttcaggttct cgtcggcttc
    53281 catccatacg tcaccaccat caccgccgtc gccgccatcc gggccgcctt tcggaatata
    53341 cttttcgcgg cggaagctca cgcaaccatt accgccatca cctgcaacga ccagaatcga
    53401 tgcttcatca acaaacttca ttttattctc cgtaaatcat tcgcctgcgc ggggttgcga
    53461 taccaccgtt gtatgcttac gtaatccgcc ccaaattcga tgaccaatgg cggaatacat
    53521 cgcgcccgca accacgacaa acgcaccgag ataacctaaa aggtttaaca tcggtctggc
    53581 gaagaaatcg ggccaggcca gtgataaaag atctgaaaaa aacagcgtaa acagtggggt
    53641 gagcgtgatg atcgcgctca cctgcgctgc ctgccagcga gccatcgctt ccgccagggc
    53701 gccatatcct accaatgtat tcagtccgca aaaaattaaa catgcgagct gccagtggct
    53761 aagctgcgct atcactccag gatttgccag agggaagagc gcaattgtac ataaagtgta
    53821 cagcaaaaac aggatctgcg gtgaggccag ccgacgcaat aaaacctttt gcgccacgcc
    53881 ataactcacc caaaccgtcg ccgcaccgac cccaaagata actccccagg tgtaatcggt
    53941 gagctttgta aatatctcga ccagactggt gttaaaaaac atcaccaggc cgctcaggag
    54001 catcaatgcc cctacaacct gagtactgcg cattttctct ttcaggataa atacgctggc
    54061 aaccatcatg ccaactggtg agagttgccc aatcacctgc gaagcggtcg gactcaggta
    54121 ttgcaaggat gagctgaaca ggatgaagtt cccaaacagc ccggcggtcg ccactgccaa
    54181 caaaatcaac cagcgtggct tacgaaacac gcgtaatggc ggcaaccgct tcttcaccgc
    54241 aagaatggca ccaaggccaa tactcgccat caagaaacgg taaaacacga ttgtcggagg
    54301 ttccatcacc tccagcacct gcttcattgc gattggcaac gccccccagc aaattgctgt
    54361 ggtgagcgcc aaaagaatgc caatgcctgc ctgctgcttc ataccgtatt ccctgcaaag
    54421 cgcatttgcg gattaacgtc gcaccatgcg catttttacc ggttatcgaa tgtaaaaagc
    54481 cccgcaacgt gttgcggggc tttcatccgt taccgggacg cgaaaaactt attcagcttc
    54541 gatgctgata aatttacggt ttttcgggcc tttaacttcg aatttcactt taccgtctgc
    54601 tttagcaaac agagtgtggt cacgaccgca acctacgtta gcgccagcgt ggaatttggt
    54661 accacgttga cgaacgatga tgctacccgc cagaacggat tcgccaccga aacgcttaac
    54721 gcccaggcgt ttagcttctg aatcgcgacc gttacgtgtg gagccgccag cctttttatg
    54781 tgccatttga aatctctcct caggtcttag gcgctgatgc cagtaatttt cacatcagtg
    54841 aaccactgac gatggccctg ctgcttacga tagtgtttac gacgacgaaa cttaacgatt
    54901 ttaactttct cgccacgacc gtgagcaaca acttcagctt tgattacgcc gccatcaacg
    54961 aaaggaacgc cgattttgac ttcttcaccg tttgcgatca tcagcacttc agcgaactca
    55021 acagtttcgc cagttgcgat gtccagcttt tccaggcgaa cggtctgacc ttcgcttact
    55081 cggtgttgtt taccaccact ttggaaaacc gcgtacatat aaaactccgc ttccgcgcac
    55141 accttttcaa tgattcagag tgcgctataa atattcacaa tagggcgcga atattacgca
    55201 aaacgcacgc ctttgacaag tgctacagtc aatacacgaa gaaaaaaaac acaacttgta
    55261 cggtaacgtt tatctgtgcc attttttcag tacaatcacc ctatattcct aaccataaac
    55321 cctaagttgc ctttgttcac agtaaggtaa tcggggcgaa aagcccggct tttgcgatga
    55381 atttagaaaa aatcaatgag ttaaccgcgc aagatatggc gggtgttaat gcggcaatcc
    55441 ttgagcagct taattccgac gtccaactga tcaatcagtt aggctattac atcgtcagcg
    55501 gcggcggtaa acgtattcgt ccgatgattg ctgtactggc tgcacgagct gttggctatg
    55561 agggaaatgc gcatgtcacc attgctgccc tgatcgagtt tatccacacg gcgactctgc
    55621 tgcacgacga cgttgtggat gaatcagata tgcgcagggg taaagctacc gccaacgccg
    55681 catttggcaa tgccgccagc gtgctggtag gcgattttat ttatacccgc gcgttccaga
    55741 tgatgaccag cctcggctcg ctcaaagtgc tggaagtcat gtcagaagcc gtaaacgtca
    55801 tcgcagaagg tgaagttctg caactgatga acgttaacga tccggacatc actgaagaaa
    55861 actacatgcg cgttatctat agcaaaaccg cgcgtctgtt tgaggctgcc gcgcagtgtt
    55921 ccgggattct ggctggctgt acgccggagg aggagaaagg cctgcaggat tatgggcgct
    55981 atctcggcac tgctttccag ttgatcgacg atttactcga ttacaatgcc gatggcgaac
    56041 agttaggtaa aaacgtcggc gacgatctga acgaaggtaa accgacgctg ccgctgctgc
    56101 atgcaatgca tcatggcaca ccagaacagg cacagatgat tcgtaccgcc atcgaacagg
    56161 gtaacggtcg ccatcttctg gaaccggttc tggaagcaat gaacgcttgt ggatctcttg
    56221 aatggacgcg tcagcgtgcc gaggaagaag cagacaaagc catagcagcg ttacaggtgc
    56281 tcccggacac cccttggcga gaagcactca tcggcctcgc gcacatcgct gttcaacgcg
    56341 atcgttaatc ccctcccctc atcccgcgca gcgcgcggga tgattccagt aaattccata
    56401 aaaacactta ttcagctcta taacacctga aaaatagggc tgtacatctt tatatattct
    56461 gaatattcac actctttaca ggaacttttt agagcaatgg gccatcagga gtatagtgat
    56521 gctcgacaga agaagtgttc tgaatgaaag cgactaactt aaggagtgag gaaagatgga
    56581 aagtaatttc attgactggc atcccgctga catcattgca ggtttgcgca agaagggaac
    56641 gtcaatggcg gcggagtctc gcagaaatgg tttgagttcc tcaacgctgg cgaacgcatt
    56701 atcacgccca tggccgaaag gagagatgat tattgcgaaa gccctgggaa ctgacccctg
    56761 ggttatctgg ccatcacgct accatgatcc gcagacccat gagtttatcg acagaacgca
    56821 gttgatgcgt agctacacta aaccgaaaaa atgagtggtc tggcggtagc cccgcgaacg
    56881 gggctgccag ctctcagaca attactcgcc tttcacacgc tcaatatttg cacctaaagc
    56941 gcgtagtttg tcttcaatgc gttcgtagcc acgatcgatg tgataaatac gatcaaccac
    57001 cgtcgtccct tccgcaatac agccagccag caccaggctt gctgatgcac gcagatcggt
    57061 tgccataacc tgtgcgccag aaagtttttc aacaccgtga caaataacgg tattgctttc
    57121 gatttcggcg tgcgcgccca tacggctcag ctctggcaca tgcataaagc ggttttcaaa
    57181 gaccgtttcg gtgatgaatc cggtcccttc tgccaccagg ttcaacagcg tgaactgggc
    57241 ctgcatatcg gtcgggaatg ccggatgcgg cgcggtacgt acgttaacag ccttcggacg
    57301 tttgccatgc atatccaggc taatccagtc ttcgccgact tcgatgtccg ctccagcgtc
    57361 acgcagtttc gccagcacgg cgtccagagt atctggctgc gcgttacggc agataatttt
    57421 gccgcgagag atcgccgccg ccaccaggaa agtaccggtt tcgatacgat ccggcagcac
    57481 acgatagaca ccgccgccta aacgttccac accttcgatg acgatacgat cggtgccctg
    57541 accgctaatt ttcgcaccca gcgtaatcag gaagttcgcg gtatcgacga tttccggttc
    57601 acgcgctgcg ttttcaataa tcgtggtgcc ttccgcaagg gttgcagcac acatgatggt
    57661 caccgttgcg ccaacgctga ctttatccat cacgatatgt gcgcctttca aacgaccatc
    57721 gacggaagct ttaacgtaac cttcttccag tttgatggtc gcgcctaatt gttcgaggcc
    57781 agaaatgtgt agatcaaccg gacgtgcgcc gatcgtacaa ccgccaggca gtgaaacttg
    57841 cccctgacca aagcgcgcta ccagcggccc cagcgcccag atagaagcac gcatggtttt
    57901 aaccagatcg taaggtgcgc agaatacatt aacgtcgcgg gcatcaatat gcacagaacc
    57961 attacgttct actttcgcac ccagctggct tagcagcttc attgatgtat cgacgtcttt
    58021 tagtttcggg acgttctgga tctctaccgg ttcttccgcc agtagtgcgg caaaaaggat
    58081 aggcagagca gcatttttag cgccggaaat tgtgacttcg ccctggagct tcgttggccc
    58141 ctgaacacga aatttatcca tttagtttgt tctcagttaa caattcatat ccgctaccgg
    58201 cgaatcgccc atagctcaaa agccgttcag tttgcgatcg cgcgcccact ccgcaggggt
    58261 atacgctttg atcgacacag catgaatgcg gttatccgca atatattcca tcagcgggcc
    58321 atagaccgtc tgctgttttt taacccgact catgccgtca aacaactcac ccacggcaat
    58381 aacctgaaag tggctgccat cgccggaaac gtggacttcc tggagggaga gagcgttcat
    58441 caacacgctc tgaatttcat tattttccat gggatcttca atcatcagtt aataaatcag
    58501 cgaaacatct tagagcaaag ttgcgctggc ataaataagc aaaaagcctc gctgataaat
    58561 cagacaaggc tcgacttgca ggcaggtttg ccggacaggc ggttaacgcc atatccggcc
    58621 tgaaaaaatt taacgaggca gaacatcagc aggcaaatta tacaatttcg ccagggtata
    58681 cactttgtcg tttaccccct gaagcgtcac attgttgccc tgctttttcg ccagatcgat
    58741 aagatggagc agcagtgcca gtccccccgt atccacgcga gacacacggc taagatcgat
    58801 gctggtaatc cccttcaccg cttcctcacg tatttcccaa agcggtagca gaacgtcctg
    58861 atccagctct ccggataacg ccagcgtgtc acccgtctgc atccagctca gtgactcgct
    58921 cattattttt tctcttccag agtgattttc tgttgagaaa tcgatttcag ttgcgcagtc
    58981 aggccgtcga tacctttggt acgcagcagc gttccccact cgttttgttt ggtggtgatc
    59041 atactgacgc cttcagcaat catgtcgtaa gcctgccagt tgcccgtctg ggagttttta
    59101 cgccactgga agtccagacg caccggcggg cggccattcg ggtcaataat ggtaacgcga
    59161 atcggcacaa tggttttatc acccagcggc tgttctggcg caatctgata ggtttgaccg
    59221 tgatacatcg ccagcgcctg accgtaagcc tgcttcaggt actcacggaa agcggcaaag
    59281 taagcatcac gttgagctgg ggtcgcactc ttgtaatact ggcccagcac cagcgcaccg
    59341 gcgtatttca cctgtacgta tggcaacagt tcctgatcaa caatggtacg cagataatcc
    59401 gggttagccc gaatttgcgg ttgctcattc ttcaggcgat cgaacgtttt ctgcgccgcc
    59461 tcgtccatca gcttatacgg attggtctgg tctgccgcgg ttgccgcact cagcggtgca
    59521 atcaccagca aagcgaccat cattaaacgt ttaaacatgc gtcggttctc ctgaaattat
    59581 ttcgttgtac ccacaggttc agtggtttca ttattacctg gcgcagcagc tggcgcatcg
    59641 ccactattct tattgtcatc gcctttacta ccgtaaagga actgaccaat gagatcttcc
    59701 agcaccatcg cagacttggt gtcctgaatt gtatcgccat ccttcaggat agcagttccc
    59761 agttccgggt cttcaaaacc gacgtttaat gccagatatt gttcccccag caggccggaa
    59821 gtacgaatgc tcagcgaact ggtatcagga atgtggttat aacgttgttc aatttccagc
    59881 gttacgcgcg gcagataggt tttcgggtcc agcgtaatat ccgccacccg gcccacaaca
    59941 acgccaccaa tgctgaccgg agagcgggct ttcaggccgc caatgttatc gaacgtcgca
    60001 taaagcgtat aggtcggttc agtacgtata gacgtcacgt tcgctgcctt caggcaaaca
    60061 aacagcgccg caagcagtgc tgctaataaa aagataccca cccaaatttc attttttttc
    60121 gtttgcatga actcaattcc caaacatcaa tgcggtcagc acaaaatcca gccccagaac
    60181 agccagagac gagtggacaa cggtgcgagt ggttgcccgg ctaatcccgg cagacgtcgg
    60241 gatggcgtcg tagccgttaa acaacgaaat ccacgtcacc gtgatggcga acaccacgct
    60301 cttaatcaga cagttgacga gatccatacg ccagtcgacg gcattttgca ttgccgacca
    60361 gaagaaccca ctatcaatgc ctttccaact gacgccgacc agcgatccgc cccagatccc
    60421 cacggcgacg aagataaccg tcaacagtgg taatgaaata actccagccc agaaacgggg
    60481 agaaataacc cgacgcagcg gatccaccgc catcatctcc atactggaga gttgctctgt
    60541 agcgcgcatc aggccgattt ctgcggttag cgccgaacca gcacgcccgg caaacaacaa
    60601 cgcggcaacc accggcccca gttcacgcag tagcgataac gccaccagca tacccagact
    60661 ggtttccgca ctataagtgg tcagaaccag ataaccttgc agccccaaca ccattccgat
    60721 gaacacgcca gaaacaacaa taatcagcat cgacaggacg ccgacattat agagctggcg
    60781 caccagcagc ggtgcatgtt tgcgaaattc cggtttgccg accagcgcat tgaataacat
    60841 taacccggcc cgcccgaacg ttctcagggt tttaatccct ttatgtccga gcgacgccag
    60901 cgcatttaac agcatgagtg gcttaactcc ctggtaaaag atcagcgtga taatcgccgg
    60961 caggatagcg gaacggaaca ggcccgtcag ctatcccgtc cagaaactga cgtacgcgcg
    61021 gatcaggatt cgcctgcaac gcctgggcac tgccatgagc gacaattttt ttgtccgcca
    61081 ggatccaggc gtgatccgca atacttaaca cttccggcac atcgtgagaa accaccacac
    61141 aagtcacgcc cagcgcgctg ttcagctcag aaatcagctt caccagcacg cccatggtaa
    61201 tgggatcttg cccaacaaag ggctcatcaa acatgatgag atccggctcc agcgcaatcg
    61261 cacgtgccag cgctgcacgc cgcgccatcc caccggaaag ttcagaaggc ataagtttag
    61321 ccgctccacg cagccccacg gcctccagct tcatcatcac cgtactatgc aacaatggcg
    61381 cgggaagttg ggtatgttcc cgcagtggat aggcgacgtt gtcaaatacg ttcatatcag
    61441 tgaacaacgc cccggactga aataacatgc tcatccgttt gcgcactgta tacaggcgcg
    61501 aacgagacat cgccggaata ttctcaccat cgaaaaggat ctcaccatga tctggtgcga
    61561 tttgcccgcc aatcagacgg agtagcgtcg ttttaccgat gcccgatggc cccatgatcg
    61621 ccgtgatctt ccctcgcggc acggtcaggg aaatattatc gaagatgcag cgattgccac
    61681 gcgtaaaact gacatcgcgc atatcgacta aattcgccac agactgctcc ataattcacc
    61741 cttcgtcttg cgttgatttt ctaagcatgg cgctcaattt aaccttgaac ccaacatatt
    61801 tacagaatat tacctgccgt ggttagcgaa agctggcatt tgttttactt tttagccgca
    61861 taaagtcaaa attaagaatc tgttacggct ttctgaaaat cttcagcgga ccggcgagta
    61921 tacctgaaga aaggacgtta gatgctttta gctacggcac tgttaattgt tggtttactt
    61981 ttggtcgttt acagtgccga ccgcctggtt tttgccgcgt ctattctttg ccgaaccttt
    62041 ggcatcccgc cgttgatcat cggcatgacg gtggtcagta ttggtacatc gttaccagaa
    62101 atcatcgtct cgcttgccgc gtctttgcac gaacaacgcg atttagccgt tggtacagcc
    62161 ctcggctcaa acattatcaa tatattgctg atcctcggtc tggctgcgct ggttcgtcct
    62221 tttaccgtcc attctgatgt tctacgccgt gaattaccct taatgttgtt ggtcagcgtg
    62281 gtggccggtt ccgtactcta tgacggacaa cttagtcgca gcgatggtat ctttctcctc
    62341 tttctggctg tgctatggct gctgttcatt gttaaacttg cacgtcaggc tgaacgtcag
    62401 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgaagg tggattgccc
    62461 gtcgcatttt tatggctcgg catcgcactg atcattatgc cagtggccac gcggatggta
    62521 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatgggtctg
    62581 acggcaattg ctatcggaac cagcctgccg gaactggcaa ccgcaatagc gggggttcgc
    62641 aaaggtgaaa acgacattgc ggtcggaaat atcattggcg caaacatttt tattattgtc
    62701 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    62761 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    62821 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    62881 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    62941 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    63001 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    63061 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    63121 cgggcgcaaa atggcagcaa catttgccag caccggtaca ccttcatttt tcgtccatcc
    63181 tggcgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    63241 ctctaactca ggtgaatcca gcgaaatcac ggccttgatt ccagtgctta agcgtcttca
    63301 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    63361 gcatctgtgt gttaaagtgg cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    63421 cacaaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    63481 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    63541 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    63601 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    63661 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    63721 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    63781 cgtgcgccca ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    63841 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    63901 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    63961 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    64021 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    64081 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    64141 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    64201 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    64261 gatctgctgg aaaaactggc gattgccccg gaaaatgtgg cttatgtcgg cgatgatctc
    64321 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    64381 ctgttgatcc cgcgcgccga ttacgtaacg cacattgctg gcggtcgtgg cgcagtgcga
    64441 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    64501 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    64561 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    64621 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    64681 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    64741 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    64801 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaatgcac
    64861 tcgtgccgga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    64921 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    64981 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    65041 cattctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    65101 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    65161 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggcaatgtc
    65221 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    65281 ggcgaacaag gtaaagaagt gattgacggc tacggtaaac cggcaacgtt ctaccagatg
    65341 caggacaacg gtaaacccgt tgaaggtcac gcttcacaga tgcactacga actggcaaaa
    65401 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgacagcaa cattaagggc
    65461 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    65521 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaataa aggccagacc
    65581 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    65641 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    65701 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgtt
    65761 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    65821 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cgcaggaagc ctccattttc
    65881 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    65941 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    66001 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    66061 gcactggctg caaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    66121 atctcggtta tcgacattaa acgcatcatt gagcacctgc gcgacagcgg cctgggcgta
    66181 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    66241 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaatacgtt
    66301 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    66361 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    66421 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    66481 ttcagcagga gctacagcag gcgctggaga gcaatccgct gcttgagcaa atcgacactc
    66541 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac actggacacc gccgacgcgc
    66601 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    66661 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctatc
    66721 agggcgaaac gacacagacc ttacaggatt acctgatgtg gcaggtcgag ttgacaccgt
    66781 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gacaccggtt
    66841 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag attgacatcg
    66901 acgaagttga agccgtcctt aagcggatcc aacggtttga tccggtcggt gtggcggcaa
    66961 aagatctgcg tgactgcctg ctgatccaac tctcccaatt cgataagacc acgccatggc
    67021 tggaagaggc aagactgatc attagcgatc atctcgatct attagccaat catgacttcc
    67081 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    67141 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    67201 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    67261 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    67321 atggtgatag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    67381 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    67441 ccttctttga gcaaggcgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    67501 ccgtcgagat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    67561 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    67621 gcgaagcttc ctccacggcg attcgtgcgc tggtgaagaa attaatcgcg gcggaaaacc
    67681 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    67741 tggctcgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    67801 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    67861 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagccaaatt tgccaaactt
    67921 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    67981 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    68041 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    68101 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    68161 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    68221 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    68281 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    68341 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgg cgcctcaggt ggtttttgaa
    68401 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    68461 catggcaaac tggaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    68521 cctatagctt tcgatgccat cgacaaccaa ccggtagact tactttttgc cctgctggtg
    68581 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtagcgaa acgtctggcg
    68641 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    68701 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    68761 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    68821 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    68881 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gagagatttc tgccgccgtc
    68941 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    69001 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    69061 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    69121 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    69181 gtcgatacct cagaaatgtc cgttcacgag ctggcagaaa tgctgcgtac ccgtctgctg
    69241 ggtaaacgtg aacgtgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    69301 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    69361 aaactgcgcc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    69421 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    69481 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    69541 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    69601 tcacgccatc ggacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    69661 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    69721 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    69781 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    69841 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taactctggt tttgatgaag
    69901 attaatcttc atcactttga cattcaaatc ccttcaagct cccccggcaa taagaaaagc
    69961 gcgtactgaa atcgttatca atcaacgtcg attgtacatt tcgtgcgctt ttttactttc
    70021 agaagaacct taagaaaacc ttaagaagca ttgtttagat ttcgtttaag ttaatcgacc
    70081 atactggaga tcgtcagaaa atatttccag gagatggcat gattcgctta tctgaacaaa
    70141 gtcccctggg caccggaaga catcggaaat gttatgcgca tccggaagat gcccaacgct
    70201 gtatcaagat tgtctaccat cgtggcgatg gtggcgataa agagatccgc cgtgagttaa
    70261 agtactacgc gcatcttggt cgccggttaa aagactggag tggaataccg cgctatcacg
    70321 gtaccgttga gacggattgc ggaaccggtt atgtctacga tgtgattgcc gatttcgacg
    70381 gcaagccttc cattacgctg accgaatttg cagaacaatg ccgttacgaa gaagacattg
    70441 cacaactgcg ccagttactg aaacagctga agcgttattt gcaggataac cgtatcgtga
    70501 cgatgtcgct gaagccgcag aatatcctct gtcaccgcat cagtgagtca gaggttaccc
    70561 ccgtcgtctg cgacaacatt ggtgaaagca cattgattcc actggcgacc tggtcgaagt
    70621 ggtgttgtct gcgtaagcaa gagcggttat ggaaacgatt tatcgcccaa ccggcgctgg
    70681 ctattgccct gcagaaagat ctgcaacctc gtgagagcaa aacgctggcc ctcacttcgc
    70741 gcgaagctta atcaagctgg tgttgttgca taaacggctc accgcctaac tgatacatct
    70801 gccgtaaaat ccacgcctga cggctacgca cgtagcccga tggcgcggag actttgaaac
    70861 gaagtggatt aggtaataca gctgccagca atgcagcttc cgaccgggta agtttgctcg
    70921 cgggtttgtg gaaataacgt tgtgccgcag cttcgacgcc aaacacaccg tcgccaaatt
    70981 cggcgatatt caggtaaacc gtcaggatac gctttttgct ccagactgtt tctatcccca
    71041 gcgttaatcc ggcttccagc ccttttcgaa cccagctacg cccatcccat aaaaagagat
    71101 ttttggctgt ctgctgagaa atcgttgaag caccgcgaat acggttttca ttgcgctcgt
    71161 tgtgcgccag ggcttgctca atggaagcga catcaaagcc ccagtgctca ggaaatttct
    71221 gatcttctgc ggcaatcacc gccagtccca tccacggcga gatttgatcc atactgaccc
    71281 agtcagaatg tgccacgtaa cgaaaattgc catgcagcca ggcgctgact tgccgctcga
    71341 ccattaccgc tgagaaagga acaggcgcaa cgctaaacaa cgcgatgccc ccgccccaga
    71401 aaatggcgag gacaaccatt aaccgcagaa gaaaacggcg aacgaaacta aacaccgtta
    71461 agcggctttt actcattcag ccagaaccag cacgcgggaa accagcttat caatgccgct
    71521 cgccgcttct gcaatgttct gcgccagcat atatgccggg gtggtgacaa tcttattgtc
    71581 ttcatcaacc acgatatcat cgacaggaca cggcacatgc tccgcgccca tctcttccag
    71641 cacttctgcg gtatcgatat cagtaccgat ggtcaaacgc agcgggaaat cgaaaatttt
    71701 cggcagtatc gccggtgcta tacacataaa accaagcggt tttccggcct gatgcatcgc
    71761 ttgtgccagc gcctttaatt cacggtcaac ggtgcattcg ctaccaagac tggcaaaatt
    71821 gcttaaattc ttcgccgcgc caaaaccccc cggcacaatc aacgcatcca gttcagcggc
    71881 atcggcctgg gccagaggac ggatttcacc gcgcgttatt cgtgccgctt caatcagcac
    71941 attgcgcgtt tccgtcatcg cttcaccagt taaatggttg ataacatcaa cctgctgctt
    72001 atccggtgca aagcagaccg cctgcgcacc gctgcgtgaa atagctaaca acgtcaacac
    72061 cgcttcatga atttcagaac cgtcatagac gccgcatccg ctcagaatta cgccaatttt
    72121 cttcattgtg atcttccttt cgcaattgac tgaaacacat taattttttt aataaaaatg
    72181 ctacgcatca cacatttaac tgattcatgt aacaaatcat ttaagttttg ctatcttaac
    72241 tgcgtgcggc ctgaaaaaca gtgctgtgcc cttgtaactc atcataataa tttatggcgc
    72301 agccaagatt tccctggtgt tggcgcagta ttcgcgcacc ccggtctagc cggggtcatt
    72361 ttttagtggc ttttgccacc cacgctttca gtacttctac gtcgtgacgc cactcttctt
    72421 tcatctcttc aatccattca ccgacgttat cttcccaggc gggaaggtca ggagactgaa
    72481 tttgctgacc cagctgttgc agatggcgta accccactga ccccgccgca cctttaattt
    72541 tatgcccttc ctcaacaatg ccttttttat cctgcgccgt cagattcgac tccagcacgc
    72601 tgacatagcc cggcatcatt ctctcaaaca ccgctaaccc gtcggtgatc agcttcggtc
    72661 ctacaagttc gagatactgt tccagcatgg gaatatcgag caatgcttct gatttactgt
    72721 tctcttctgt cgtcaccgta ctctcctcat catcctgggt atcccagaat ttcttgatca
    72781 tcgcggttag cgccggaaca gaaagcggct tactcagcac atcatccatt ccagcattga
    72841 ggtactcttg tttgtctttc agcacgttag cggttaaggc caccagcggc ggtaaatcct
    72901 cgcgcggata acgcttcgtc agtgcacgag agatatccaa cccggtcata tctggcaact
    72961 gaatatccag caacaccagg tcgtattcgc ccggtttaaa catctccagc gccgccttgc
    73021 cggtcatggc gacatcaacg ctgttaccta acttttccag cacagagcgc gcgacaatca
    73081 cgttcagttc aatgtcttcc accagcagca cattcagcgc cggtaaaggc atatcgtctt
    73141 catcaaacgc atcatcaacc tcttctgcta ccgacggtgc gtggatcgtc aacgtaaagg
    73201 ttgaaccttt gccctgttcg ctggtaaccg taatatcgcc gcccatattt ttcgccagac
    73261 gacgagaaac ggccagacca ataccggtgc cggtggcagg tttaccgcca tgactgtctt
    73321 tcacctggta atacatggca aagattttat ccagctcatc ctgcggaatg ccgataccag
    73381 agtcttccac ttcaaaatgc agcatatcgc cttcatcgta gcgcacgcgc acggtaacct
    73441 ggccttgctg agtgaatttg acggcgttac tgatgaggtt ccacaagatc tgccgtaaac
    73501 gcgtcccgtc ggtaatgacc tgatgcggta atggcagcgt cggctccaga ttaaagcgca
    73561 atcctttttg ttgcgcctgc aatgcggaga gattttccag atcggcaagg aagctggtga
    73621 aatcaaccgg ttgattatca agctggacct tgcgccgttc catcttatcc atgtcgataa
    73681 tatcgttgaa gatattcccc agcgtgacag ccgaaacatg gatggttttg agatattttt
    73741 cctgctcggc ggtgagttca gtatccagca gaatgcggct caggccgacg ataccgttca
    73801 gcggtgtacg caattcgtga ctgatggtgg agataaacgt cgttttgtcg cggctggcgc
    73861 gttcaagcgc atcctgatac cgcttacgct cggtaatgtc gcgaccaaag cccatcaaac
    73921 cgtgacgttt acccacgcgg tcgtagtacg gtactttacg gatttcaaag caggctttgc
    73981 gtccgtcggg ataatccagc cactgctcat aggtcagtga cacattatga cggaacactt
    74041 tttcatcggt ttcaatgact ttcgcagcgg cttccggtga gtaaacatca gcaggtttca
    74101 ggtgaaccag ttgtttttcg ctttttccgg tcagcagctc catcgcgcgg ttacagccgg
    74161 aaaactcttt atcttcgtta cgataaaaaa ccaggtcggg tgaagcgtca aggaaggaac
    74221 gtaagaatga ggattgctgc tcgagctgaa tttgtgtctc ttcgcgctct ttgatttcaa
    74281 ttttcagttg gccgaaggtt tcctgcagtt ctgcttccgc tttttcacga acggcgattt
    74341 cctgatttag ctgggcaata ttatctttta actgaacgtt gaggctcaaa tcgcgctcgc
    74401 gcatctcctc cagtttttgc accagtcgtg acagacgttg tcgtgactcc tccagttgct
    74461 cgacgaccac cgatagaaag tagaccgccc acggcgtaat cagcaaacca aagaagatag
    74521 aacgaataac atcaatgctt tcgacctggc catgcagcac catggttacc gccatttgta
    74581 ccacaatggc aagaacgacg agggccagcg ccagcaacat tgagaagcgc accagaccta
    74641 acttcatcat caggtcaaca taatactgcg ccagcagacg aatttgcttc attagggaat
    74701 tccttcacga caacctgccc caataatacc caattctgac agttacgtta taaattgtgt
    74761 gagaaatgcg gaattcccct caatggccta cgccgttgga ggtagccacg gacgtcccag
    74821 cgccgatccc tgtacgccat gttcattcag ataacgatcc agcccgacca ttcccgtcca
    74881 tcgattttcg caccacagcg gagcaagcag cgtcgggcga cgggcgctgg cggaaatgcg
    74941 gtggtagatc acttccggtg gcgtatggcg aatcatttct ccggcagtga gcgtgtaatc
    75001 ctccagttca attccgttca aacgccccgc ttcccaggct ttcgccataa tgctgccttt
    75061 cactatatgc agcggatgca gctttatgcc gtccacgccg gtttcaacca ctcgctccag
    75121 cgtttgcaaa cattcagcct gcccttcgcc aggcaggcca acaattaagt gagaacacac
    75181 cttcagccca cgctcacgcg ccagctgcgt cgtgcgctga tagcaggcaa aatcatgacc
    75241 gcgattgatg cgatgcagtg ttttgtcgtg ggcggtttgt agccccagct ccagccacac
    75301 ttcgtagccc tggtccttat attcgcaaag cagatccagc accgcatccg gcacgcagtc
    75361 cgggcgggta ccaacacaca aaccgacaat attggcctgg ctcaccgcct gctgatacat
    75421 cgaacgcagc acctgaactt ccgcaaaggt actggtatac gcctgaaagt aggccagata
    75481 gcgtttagcg cggttaacta aattcgcctg gtgcgccagt tgctcggcaa tggaacgatg
    75541 ctgctgcgct tcatcggcaa acgaggcaac attacagaat gtgcagccgc cacgcccgat
    75601 ggtaccgtca cggttagggc agctaaaacc gccatgcagc gtcagcttat gcaccttttg
    75661 cccataacga cgggtgagat caccaccaaa catattgact aatttctgta actgcataat
    75721 ctgatagacc gcgccttgaa aagaggccaa agcctgccat ttttagccca attcggcgat
    75781 gacctggatc aatcgtcccg gcctgctttt atcaactgca taatcaatca aaattaccga
    75841 aatttcatgc acaatcacat aaatcacttt tgcttatctt gtgtcagatt tttttatctc
    75901 cttatggatt ttaggcaaaa acagtagcat gaaacgttat taccaattaa ggcagtataa
    75961 aatgctggtt ttctcgtcag ttcaaggcag gataagggtt aacacgcctt tatgacagtc
    76021 aggtattggc tgtttctcta acgacgcccc ttttagcctt aaatagaaaa tccaatttaa
    76081 tttcagataa ttaataaaga attttgcgct aaagcacatt tctgtaccaa taagcttgcc
    76141 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
    76201 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
    76261 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
    76321 acgctcttac tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
    76381 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
    76441 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
    76501 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggtgac ggttgcggct
    76561 tgctgttaca aaaaccggat cgtttttttc gcatcgttgc gcaggagcgc ggctggcgtt
    76621 tagcaaaaaa ctacgccgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
    76681 ccgcacgccg catcgttgaa gaagagctgc aacgcgaaac gttgtcgatt gtgggctggc
    76741 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccacgca
    76801 ttgagcaaat ttttgtgaac gccccggcag gctggcgtcc gcgcgatatg gagcgccgtc
    76861 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
    76921 gtagcctgtc gaatctggtc aatatttata aaggtctgtg tatgccggcg gatctgccgc
    76981 gcttctacct ggacctcgct gacctgcgtc tggaatcggc catctgcctg tttcaccagc
    77041 gcttttccac taacaccgta ccgcgctggc cgctggcgca gccgttccgc tacctcgcgc
    77101 ataacggtga aatcaacacc atcactggta accgccaatg ggcgcgtgcg cgtacctata
    77161 aattccagac gccgctgatc cctgacctgc acgacgccgc gccgttcgtc aacgaaaccg
    77221 gctctgactc cagttccatg gataacatgc tggaactcct gctggcaggc gggatggata
    77281 tcatccgcgc catgcgtctg ttagtaccac ccgcctggca gaacaacccg gatatggacc
    77341 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggcccgg
    77401 caggcatcgt gatgtctgac ggtcgttttg ccgcctgtaa cctcgaccgt aacggtctgc
    77461 gtccggcgcg ctacgtcatc accaaagata agctcatcac ctgcgcctct gaagtcggta
    77521 tctgggatta tcagcctgac gaagtggtcg aaaaaggccg cgtggggcca ggcgaactga
    77581 tggttatcga cacccgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
    77641 aaagccgcca tccatataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
    77701 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
    77761 accagaaaca gtttaactac agcgcggaag agctggattc ggtgatccgc gtactgggtg
    77821 aaaacggtca ggaagcggtc ggttcgatgg gcgacgacac cccgttcgcc gtgctctcca
    77881 gccagccgcg cattatttac gactacttcc gccagcagtt tgctcaggta accaacccgc
    77941 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagcatt ggccgcgaaa
    78001 tgaacgtctt ctgcgaagct gaaggccagg cgcaccgttt aagctttaaa tcgccgattc
    78061 tgctctattc cgatttcaaa cagctcacga cgatgaaaga ggatcactac cgcgcagata
    78121 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacggtc aaagagctgt
    78181 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct gctggtgctc tccgaccgga
    78241 atatcgcgaa agatcgcctg ccggttccgg ctccgatggc ggttggcgcg atccagaccc
    78301 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
    78361 gtgatccgca ccacttcgcc gtgttgctgg gtttcggcgc gacggctatt tatccgtacc
    78421 ttgcctatga aacgctgggc cgtctggtag acacccacgc gattgccaaa gattatcgta
    78481 ccgtgatgct caactatcgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
    78541 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctgc
    78601 acgatgatgt cgtgggcctg tgcttccagg gggcggttag ccgcattggg ggggctagct
    78661 ttgaagactt ccagcaggat ctgctgaatc tgttgaaacg tgcctggctg gcgcgtaagc
    78721 ccatcagcca gggcggcctg ctgaaatacg tgcacggcgg cgaataccac gcctacaacc
    78781 cggacgtggt gcgcacgctg caacaggcgg tacagagcgg cgagtatagc gactatcagg
    78841 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
    78901 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
    78961 gcttcgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
    79021 cggaagcgat gaacagcatc ggcggtaact caaactccgg cgaaggcggt gaagacccgg
    79081 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcgtccggt cgcttcggcg
    79141 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
    79201 agccgggaga aggcggtcag ttgccgggtg ataaagtcac tccttacatt gccaaactgc
    79261 gctattcggt gccgggcgtg acgctgatct ccccgccgcc gcaccatgat atctactcta
    79321 tcgaggactt agcgcagctc attttcgacc tcaagcaggt taacccgaaa gcgatgatct
    79381 ccgtgaagct ggtgtccgaa ccgggcgtcg gcaccatcgc gaccggcgtg gcgaaagctt
    79441 atgcggactt gatcaccatc gcaggctatg acggcggcac cggcgcaagt ccgctttcat
    79501 cggtgaaata cgcaggctgt ccgtgggagc tggggcttgt tgaaacccaa caggcgctgg
    79561 ttgccaacgg cttgcgtcac aagatccgtt tgcaggtcga tggcggcctg aaaacgggtg
    79621 ccgatatcat caaagcagcg attcttggcg cggaaagttt tggcttcggc actggcccga
    79681 tggtagcgct cggttgtaaa tatctgcgaa tttgccacct gaacaactgc gccacgggtg
    79741 tagcaactca ggatgacaaa ctgcgtaaga atcactatca cggcctgccg ttcaaagtga
    79801 cgaattactt tgagtttatc gcccgtgaaa cccgcgggct gatggcacag ctgggcgtaa
    79861 cacgtctggt ggatctgatt ggtcgcaccg acctgctgaa agagctggac ggtttcaccg
    79921 ccaaacagca gaaactggcg ctgtcgaagc tgctggagac tgccgagccg catccaggta
    79981 aggcgctcta ctgcaccgaa aacaacccgc cgtttgataa cggcctgctg aacgcgcagt
    80041 tgctgcaaca ggcgaaaccg tttgttgatg aacgccagag caaaaccttc tggttcgata
    80101 tccgcaacac cgaccgttct gtcggcgcat cgctttcagg ctatatcgcc cagacgcacg
    80161 gcgatcaggg gctggctgcc gaccctatca aagcgtactt caacggcacc gcaggccaga
    80221 gcttcggcgt gtggaacgcg ggcggcgtgg aactgtacct gaccggtgat gccaacgact
    80281 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggctcagcct
    80341 tccgcagcca tgaagcgagc attatcggca acacctgcct gtatggcgcg accggtggtc
    80401 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcgtaactcc ggtgctatca
    80461 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggtggt atcgtctgta
    80521 ttctgggtaa aaccggcgtt aacttcggcg cgggcatgac tggcggcttc gcttacgttc
    80581 tcgatgaaag cagcgatttc cgcaaacgcg ttaacccgga actggtcgag gtcttaagcg
    80641 ttgacgatct ggcgatccat gaagagcatc tgcgtggtct tatcaccgag catgttcagc
    80701 ataccggttc ccaacgtggt gaagagattc tggcgaactg gtcaaccttc gccactaaat
    80761 ttgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcac cgtagtcgta
    80821 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
    80881 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgcaaaa
    80941 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggccgacc
    81001 gctgcctgtc gtgcggcaac ccatactgcg agtggaaatg cccggtacac aactacatcc
    81061 caaactggct gaagctcgcc aatgaggggc gtatttttga agcggcggaa ctgtcgcacc
    81121 agacaaacac cctgccggaa gtttgcggac gtgtctgccc gcaagaccgt ctgtgcgaag
    81181 gttcctgcac cctgaacgat gagttcggtg cggtgaccat cggcaacatt gagcgttata
    81241 tcaacgataa agcgttcgag atgggctggc gtccggatat gtccggcgtg aaacagaccg
    81301 gtaaaaaagt agcgattatc ggcgcaggtc cggcgggtct ggcgtgcgcg gatgtcctga
    81361 cgcgcaacgg cgtaaaagcg atcgtcttcg accgtcatcc ggaaattggc ggcttgctga
    81421 ccttcggtat tccggctttc aagctggaaa aagaggtaat gacgcgccgc cgtgaaatct
    81481 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtgcagttgg
    81541 acgatctgct gagtgattac gatgccgtgt tccttggcgt cgggacttat cagtcaatgc
    81601 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
    81661 ccaacaccaa acagttaatg ggctttggta aaacccacga cgaaccgttc gtcagcatgg
    81721 aaggcaaacg cgtggtggtc cttggcggtg gcgacactgc gatggactgc gtgcgcacgt
    81781 ctgtacgcca gggggcaaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
    81841 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
    81901 tccagccgct gggtatagaa gtgaatggca acggcaaagt cagcggcgta aaaatggtgc
    81961 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatt gtggcaggtt
    82021 ccgaacatat cgttccggca gatgcggtga tcatggcgtt tggtttccgt ccacacaaca
    82081 tggaatggct ggcaaaacac agcgtcgagc tggattcgca ggggcgcatt atcgccccgg
    82141 aagacaacga caacgctttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
    82201 tccgtggttc cgatctggtc gttaccgcca ttgccgaagg tcgcaaagcg gcggacggca
    82261 ttatgaactg gctggaagtt taagcgaggt aacaataaat tcgccagaca gtgtctggcg
    82321 aatttataaa acgagcgatc caactaaatt ttaagccatt aaatcagcct taaccttcac
    82381 taccaccttt ttaatctcac caggttcgcc aacaacgcat cccggtttat gcggttctcc
    82441 tggcataaac acggcgaaca ttcccggttt caggatgatg gcttgctcgt tttcaatggc
    82501 gctgcaaagc tgataatcat cttcatggtg gaactcttca cactgacgcg cagtgccagc
    82561 catgccaaac agaatccgtt cctcaccgtt taataacagc tgaatatcaa tgtattgttc
    82621 gtgcaattcg gcttttttct caacgggcga ttgagtgtta aacgtcatga cattcataaa
    82681 aatattgtcg ccctgtaatt cgtaacggcc aggcgctttt tcttgcggtc tggcagctaa
    82741 tgccagcgtt aacgcgccct gtaacgcagg atgtaatccg gcagacggta atgactgtac
    82801 ttcacccatc atcataatat ttctccctgg gccaacagcg cagccccaag taaacctgta
    82861 tcgtggcggt agtgcgccgc cagtaaatca acatgaaatg ccgctggctc ctgcgccaga
    82921 tacgtttcca ccagcgccag atacccttct gccagaccaa cgctgccacc gaccaccacg
    82981 cactggcaat cagttgtggc tttaatatca gcgatcagcc ttgcaagcgt acgtgcggag
    83041 cggtgaatca gttgctgcgc ctgctcatcg ccctgcccgg cgcgcgtgaa aatagttttc
    83101 gcattcgcgc cagccaactc cccctgcgct gccgctgcaa tgccgcgacc agaagcaatc
    83161 gcttccacgc aacctgtgcg tccacagccg cagactggac catgcggatc ggcaagcgta
    83221 tgcccgatat gccccgccag accgccaggg ccggtacgca gtttaccgcc gctcactaca
    83281 ccgccgccaa cgccggtgga aacagtgata aacaccatat cagttatatc gccatccagc
    83341 gcctgatact ccgcccatgc tgcggcctgc gcatcattaa tggcaatggt cggcaaattg
    83401 gtaagttgct ccagcgtttt gactaacgga aagtgtaaca atccaccgag attatgcgga
    83461 ttcagcgcca gcaagctgcc gtcgcgaata attccggttg aagcgatggc aacccgctgc
    83521 gcatgtgctt gcaacggaga gactaatgcg gataaggcat cacgcagcgc ctgcggagtc
    83581 tggctggcag gcgtaggaag ttctcggcga tcgcggatct gcccgtcagc gccaatcagc
    83641 gcggcggcaa gtttagtacc gccgatatca atcgccagtg tggtcatagc accgcctttt
    83701 tcatcgctgt gttgtaccac tgacaaatgt gttcaagacg cgtaatggca gaaccgaccg
    83761 tcaccgccca cgcgccgtgg cgcatcgcat ccgccgcctg agcaggcgtg ttgtaacgcc
    83821 cttcggcaat cacccggcat ccggcgtcac tcaacgtttt caccagcgcc agatccggct
    83881 cttctggcgt ttcaggcgtg gtatagccag aaagcgtagt gccaataatt tcggctccca
    83941 gcttttggca tgccaggccg tcttccggcg ttgagcagtc ggtcatcgcc agtaaaccgt
    84001 gatggtgaat acgtgccagc agcgtttcaa caggtaccgg acgcggtcgg tcggtgccgt
    84061 caatggcgat aatatccgcg cccgcctgcg ccagcgcatc aacatcttca atataggccg
    84121 tgatgcgtac cggagaatcc tccagatcgc gcttcacaat cccgataatc ggcacgctca
    84181 ccaccgcacg cgtggcttgc agatttgcca caccttcaat acgaatggcg accgcgcccg
    84241 cctgctctgc cgctaatgcc atggcggcga cgatttcggg tttatcgagc gggctgtccg
    84301 gaaccggctg gcaggagaca atcaggccac cgttagcagc gattttttga tccagttgtg
    84361 caagtaacga catacatctt ctcttagcga aaggcccggt acagagaccg ggcaacagga
    84421 ttaacttttg gttttgacta aatcgttttt ggcgctgcca aacggcacgg caccgctaaa
    84481 tggtttgccg tcgatagcgt catgagtacg caatgcttcc gggcgcagcc aacgctgaac
    84541 acgagaaggc atatccagac caatcagcag gatcaccaca aacgttaaac taaacgagag
    84601 cgagccaagt gccgttccca gatccagacg ttgagcgatc aacgcgccga ggattggagc
    84661 tagtgcaccg cccagtgcgc caacgttgta ggtaaagccc agccccgccg cacgctggtc
    84721 ggtatcgaaa taaccgccga tcagtttcgg taaaatccct gagatgccct gaccaagcat
    84781 ttgctggaag aacagtaaca gaccgagcac ccagacgttt gcgccgccaa tcgcaaatac
    84841 cggaataatc agcagctgcg aggccagcag actacaaacg tacgctttgc gggttcccag
    84901 ccagtcaccg aggaagccac cgacgcagca ccccaccgcc gcgccaaagc cactaaagaa
    84961 cagcacattg gcgacagtat gcgggtcata agccagatcg gttttcagat acgttggcag
    85021 cagcgcctga atcggccatg agtagaggaa agcaaacaac acgaccacca tcagcattac
    85081 gcccgttggc cagcgtttgc ccgtactttg caccataaag ctgataaaga ttgcggcgca
    85141 taacagcccg agtacagcga cgatcgcggc attttgcagg ttaccggcga agcagaacca
    85201 cagcgcagta gccgccgcca gtgtcattac gatattggca atgcgatgtt caccacggta
    85261 gagaatgtct accatcgtgc gtactggtgc tttacccccg tgtttctctt tccagtcttc
    85321 cgcttccggg atgtttttac gcagccagag agcaaagatg attggcaaaa tgccgataaa
    85381 gaacagcgct ctccagcccc agaccggaac caccagacta tagacctgag cggcaacgac
    85441 ggcccccact gagaagcctg aaatcagaaa accactggct ttgttacgca gatgctttgg
    85501 ccagctttca atgacatagg tggcgctgga accgtattca cccgccatcc ccatgccgat
    85561 gaccagacga gcgataaaca tggtgatgta gcctggcgca aagccgcagg ccagcgtccc
    85621 ggccgagaag agaacgatgc tggtgaccat tgccagacga cgcccgtagc ggtcacccat
    85681 agcgccgagc atcaggccgc cgaaccagcg agagataaag gctgcggaga tcagacttgc
    85741 cgcctgcacc gtcgtcagcc cgaattcacc ctgtacttcg gtgagtacca gggcgattaa
    85801 aacgaaatca aaaccgtcaa gcagatatcc caaccaggca gcggaaaatg cccgccattg
    85861 tgcgcggtta agatggcgat accacgggat attctgggtt gtagtactca ttgtgagtct
    85921 cccgcggtgg gcgatgccca cacgctttgg tatgaaaatt gtagggtaca gatgcgttta
    85981 tttcccctca cccggtaggg gcgagcgagg ggaaacaact cacccgcgct cttgcatcaa
    86041 ctgctgggcc agcgccttca gttctggcag atatttttca tctaccggac caaacggttt
    86101 gcggcacagc ggcacagaaa cgacatccat ataatgcagg acagttttca ggccgcggaa
    86161 tacgccagtt ttgatcagta aatcaatgac tttgttgcac tcagtttgca gtttctgcgc
    86221 ggtctggata tcgccttctt tcagcgcctt cacgatcccc tgataacgcc agcccatgat
    86281 gttgtaagtg ctgccgatac cgccgtctgc gcccgccaga agaccagagg cgaagatttc
    86341 gtcgtaaccg ttatagagca ccagatcagg atgttcacga cggatctgct ccatctgata
    86401 gagatcgcca gaggtctgtt tcagcgcacc tacaccaggc agcgtaacca gggtgttgat
    86461 ctgatccagg gtcagtttta ccccgctcag ggctggaatg ttgtacacca ccatcggcaa
    86521 cccatccgcc gaatcaataa ttgcccgata gtgatcgcag tgttcttcaa agctgaaagg
    86581 atagtagaac ggcgtgacgg cggagacggc atcgaagcca taacgtttag ccgatgccgc
    86641 aagttgttgg ctttcggcgg tgctgacgca accgacgtgg gcgatgagtt taatcttacc
    86701 tttcgcctct tcggcgacga tttccagtac ctgttcgcgc tcggaaaggc tttgtacaaa
    86761 ggcttcgcct gtcgaaccac ccacgtacaa accgtcgatg ccctgctgaa tattgaactg
    86821 aaccaggcgg cgcagactcg ctttatccag tgcttgttgt tggtcaaaag gagtcaggag
    86881 tgcagccatt acgccacgta aattcgttgc cataaatacc tctgaagtga tgcttgtctg
    86941 ataaacgata tacctttata cctgttatac cagatcaatt aagcaacacc ccatacagaa
    87001 agcttataat gcgatctgct tcactaaagt ggcattattt ctttttgttg gtggtctgac
    87061 cgaaggcgtg ccaggtagca gagacgctgt tgagatgcga ttgcaacgcg cgatcggctt
    87121 cgtcaggatc atgacggcgg atcgcgtcaa cgatcgcaat atgctgttga tagctaacgt
    87181 tgttatgctc atgcagagcc tgatcagcca cggttggacg tgcggcgatt agccagtcga
    87241 gcagggcaac gtggatcgcc atgaagattg ggttaccagg gatctccgcc agaacgcggt
    87301 ggaaatcaac gtctgagcga atgaatgccg cgttgttatc cagcgactga ctgttgattt
    87361 ccagtgcttt tgccagcaaa tcgatttgct catcggtggc gtgttcagcc gcatagcgca
    87421 ccaggctgga ttcaaagaac agacgtaatt gttcgaaatg ggcaatccca ccaggatgag
    87481 aaaggaaatc tttcgccatg ccggaaagct cgccgatgat ggtgtccgca gaaggacgtg
    87541 agacgcgggc acgttcgccg ttgtttattt gcaccagacc tttgcgtttt aacgctgcca
    87601 gcgcttcgcg caccgaagga cgcccgacgt taaagaacgc catcagttcg cgttcagacg
    87661 gtaattgttc accttcacca aattcacgac ggcggatcat ctgttccagc tcttcttcca
    87721 ccatttcgga gagtttttta cgcgccagcg ggcggctgcg caagttgcgc ccgatagtgg
    87781 tagtagaatc ttcagcttgc ggatcaaatg cgttcatagg actcattcag gaaaatgcgt
    87841 tagcggataa taacgacgct atcgtagcac agtatcaaag tcaggcgaaa agatgacaga
    87901 tagcaaaaca ggccacatgg gcctgttttc attttacttc acaacgcgta atgccggtcg
    87961 accaccgcgt ggtggctgcg gaggttcatc gtcaggatga gtttcatcat cgtgatctgg
    88021 cttgtcgcca tcgataaccg acataacggt ttcgttgtct gccgatgcct cttcatcatt
    88081 catgatgctg gcatcttcat cgtaggcagc ttcaggctca aacatcgtgc ctgcgccatt
    88141 ttcacgggcg tagatagcta gcacagcagc cagcggcaca gaaacctgac gcggaatgcc
    88201 accaaagcgc gcgttaaagc gcacctcatc attcgccagt tccagattgc cgacagcacg
    88261 cggcgcaatg ttgagtacga tttgcccgtc acgcgcatat tccataggaa cctgcacgcc
    88321 agggagcgtc acatccacca ccaggtgcgg cgtgagctgg ttatccagca accactcata
    88381 gaatgcacgc agcagatagg gacgacgtgg tgttagctgt gacaaatcca tacagattaa
    88441 ctccggccca gacgcatttc acgttctgct tcagttaaag aagcaaggaa agagtcacgc
    88501 tcaaatacgc gggtcatata gcctttcagc tctttcgcac ccgggccgct gaactcgatg
    88561 cccagttgcg gcagacgcca cagcagagga gcaaggtagc aatcgaccag gctgaactca
    88621 tcgctcagga agtacggctt ctgaccgaag accggcgcaa tcgccagcag ttcttcgcgc
    88681 agttgcttac gtgcggcatc ggcttcagaa gctgaaccgt tgatgatggt gttcatcagc
    88741 gtgtaccagt ctttttcgat gcgatgcatg tacagacggc tttcaccgcg agctaccggg
    88801 taaacaggca tcagtggcgg atgcgggaaa cgctcatcca gatattccat aatgatgcga
    88861 gattcccaca gggtcagctc acgatccacc agggtcggaa cgctctgatt cgggttgagg
    88921 tcaatcagat cctgaggcgg attgtccttt tccacgtgtt cgatctcgaa acttacacct
    88981 ttctcagcca gcacaatgcg gacctgatgg ctatagatgt cagtaggacc ggaaaacagc
    89041 gtcattaccg aacgtttgtt ggcagcgaca gccatgaaaa cctccaggta tagtcagaat
    89101 ttttactgct accagccacc aggtggccag tcagaagttg tgttacccag taaggaacga
    89161 ctctctttgt tcgaaaatca aacaaaaaat gagtaatacc cgacatttgg gcagaaaatt
    89221 ggatgatagt ttaccagatt ttgcgacctt tgtggtgagt cgatgccgga aatggggaaa
    89281 aagagatgcg ctttagtctg aaatagttga cttagtccct tattggcgat gtggtttttg
    89341 ttttacctgt ctgtcaggtg gcagcaaaaa gcaactttcc attttttacg ctgatgcaga
    89401 ttttagccat aaaaaaaccc gccgaagcgg gttttttcgc aaattgtttt ctgccggagc
    89461 agaagccaat taacgtttgg agaactgcgg acgacgacgt gctttacgca gaccgacttt
    89521 cttacgttca acctgacgag cgtcacgagt aacgaagcca gctttacgca gttcagaacg
    89581 cagggactcg tcgtattcca tcagagcgcg ggtgataccg tgacggatcg caccagcctg
    89641 accagagata ccaccacctt taacggtgat gtacaggtcc agtttctcaa ccatgtcgac
    89701 cagttccagc ggctgacgaa ctaccatgcg agcagtttca cgaccgaagt actgttccag
    89761 agaacgttgg ttgattacga ttttaccgtt gcccggtttg atgaaaacgc gagctgcgga
    89821 acttttgcgg cgaccagtgc cgtagtattg attttcagcc attgcctata atcccgatta
    89881 gatgtcaaga acttgcggtt gctgtgccgc gtggttgtgc tcgttacccg cgtaaacttt
    89941 cagtttacgg aacatagcac gacccagcgg gccttttggc aacatgcctt taaccgcgat
    90001 ttcaatcaca cgctcaggac ggcgagcaat catctcttca aaggtcgctt gtttgatacc
    90061 accgatgtgg ccggtgtggt gatagtacac tttgtcagta cgcttgttgc cggttacagc
    90121 aactttgtca gcgttcagta cgatgatgta atcaccggta tctacgtgcg gagtgtattc
    90181 cgctttgtgc ttaccgcgca ggcgacgagc cagttcagta gccagacggc ccagagtttt
    90241 accggtcgcg tcaacaacat accagtcgcg ttttacggtt tctggtttag ctgtaaaagt
    90301 tttcattaaa agcttaccca ataaatagtt acacgttggt gaacacccaa acgtcttcaa
    90361 ttgttgaggt tcacacgaca aagtccggca aacctacccc ttcgaatagc ctatgccagc
    90421 acacaaaaag tttcgggaaa aaaactttct tgtaacgtgg ggtcgcagga ttatagagaa
    90481 gtcggggtca aagatcgacc cctttttgtg atttgtggca ggttttaccc cgccaaatgc
    90541 tcgcgcttca aatactcttc gctttgcatc tcttgcagac gtgacaggca acgctggaac
    90601 tcaaacttca gccgctcgcc ctgataaatt tcatacagcg gcacttctgc actcaccact
    90661 aatttgacat ggcgctcgta aaactcatcc accagcgcaa taaagcgtcg cgcttcgctc
    90721 tccatcaacc gcgtcataac tggtacatca aacaacatga ccgtgtgaaa gaggcgtgag
    90781 agcgcaatat agtcatgctg actgcgggcg tcgacgcaca gcgtagtaaa agaaaccgcc
    90841 agcgtctggt tctcgacgcc cattgtcgcc aatggccgat ggttgatttc taacgtcggt
    90901 gaattttctc gtttcgcccc cgccagcgcc aaccatagtt tatccatttg cgcccggatt
    90961 tcatcgttga gtggcgaaag ccacaggtgc gcctgagtga gtgtacgcag gcgataatca
    91021 acaccagcgt ccacgttcat tacatcacaa tgctgtttaa tggcatcgat tgcaggcaaa
    91081 aaacgcgcac gttgcaggcc atttcgataa agttcgtccg gcggaatatt tgacgtcgct
    91141 accagggtaa taccgcgggc gaacagggct ttcatcagac cgccaagtag catggcatcg
    91201 gtaatatcag aaacaaaaaa ttcgtcaaaa cagagcacgt cagtttctgc tttaaagcga
    91261 tcggcgataa tttccagcgg atcgctttgg ccttgtaatt cggtcagctc atcgtgcacc
    91321 cgcagcataa aacggtgaaa gtgcaggcgc tgtttccgct ctcccggcag actttgatag
    91381 aaaaggtcca tcagccaggt tttcccgcgt cctacaccgc cccacatata taagccacgc
    91441 actggcatat gctttgtgtc ttcgcgttta ccccacagct taccgacccg cgccattagc
    91501 ccactcgtcc tgggggctgg tggcgtgcta ttgatgagtt cctgataaat aatttccagg
    91561 cggctgacgg cctctttttg aacgtcgtcg ggttgatggc tgccttcatt gagcgccttc
    91621 aggtattgcg aggttggggt aacgctttgc atgatcttat tgttattcct tgaataatcg
    91681 gtgcgccgtt gttcacggtt gacgaaaaaa aggccgttct acactacgcg atatgctgtc
    91741 gggattccac ttctgtggaa ttaacggtta tagtggcata atcagccgca ggcatggagc
    91801 ctgaagccaa caccctacgg aaacaaaaga caacgggaga tgttcatgac ctgggaatat
    91861 gcgctaattg ggttagtcgt cggcatcatt attggtgctg tggccatgcg ttttggtaat
    91921 cgtaaactac gccagcaaca ggcgttgcag tacgaactgg aaaagaataa agctgaactg
    91981 gacgagtatc gcgaagagct ggttagccac tttgcccgca gcgcggaatt actggatacc
    92041 atggcgcacg actatcgcca gctgtatcag cacatggcga aaagctccag tagcctgctg
    92101 ccggaactgt ctgcggaagc aaacccgttc cgtaatcgtc tggcagagtc tgaagccagc
    92161 aacgatcagg caccggtgca gatgcctcgc gactattctg aaggcgcatc cggcctgctg
    92221 cgtactggcg cgaagcgcga ctaatttatt tttcgggcgc agccattgcg ccctcctctt
    92281 ctctccctcc ccgactatca tttaatctgg tgtctcattg ttagccgtct gaaaattcaa
    92341 taacatcaaa ctgttttgaa tctcttttct tatcattcag gtacgagagc aggaataatg
    92401 aaaaaacaaa cccagctgtt gagtgcatta gcgttaagtg tcgggttaac tctctcggcg
    92461 tcatttcagg ccgtcgcgtc gattccaggc caggttgccg atcaggcccc cctccccagt
    92521 ctggctccaa tgctggaaaa agtgcttccg gcagtggtga gcgtacgggt ggaaggaacg
    92581 gccagtcagg gacagaaaat cccggaagaa ttcaaaaagt tttttggtga tgatttaccg
    92641 gatcaacctg cacaaccctt cgaaggttta ggctccggtg tcatcatcaa cgccaataaa
    92701 ggctatgtgc tgaccaacaa ccatgtgatt aatcaggcac agaaaatcag tattcagctc
    92761 aatgatgggc gcgagtttga tgcaaaacta attggtagcg atgaccagag cgatatcgcc
    92821 ctgttgcaaa ttcaaaaccc gagcaaatta acgcaaatcg ctattgccga ctccgataaa
    92881 ttgcgcgtcg gtgattttgc cgtggcggtc ggtaacccat ttggccttgg gcaaaccgcc
    92941 acctctggca ttatatccgc attaggccgc agcgggttga atcttgaagg tctggaaaac
    93001 tttatccaga cagatgcttc cattaaccgc ggtaactccg gcggtgcact gttaaacctt
    93061 aacggtgagt taattggcat caacactgca atccttgcgc ctggcggcgg tagcgtcggg
    93121 attggatttg ccattcccag taatatggcg cgaatactgg cgcagcagct tatcgacttt
    93181 ggagaaatca aacgcggcct gttaggtatc aaaggcaccg agatgagtgc cgatatcgcc
    93241 aaagccttca accttgacgt gcagcgtggc gcgtttgtca gcgaagtgtt gccaggttct
    93301 ggctcggcga aagcgggcgt caaagcgggc gatattatta ccagcctcaa cggcaaaccg
    93361 ctgaatagct ttgctgagtt gcgctctcgt atcgcgacca ccgagccggg cacgaaagtg
    93421 aagcttggcc tgctgcgtaa cggcaaacca ctggaagtag aagtgacgct cgataccagc
    93481 acctcttcgt cggccagcgc tggaaggtgc aacgttgagc gatggtcagc taaaagatgg
    93541 tggcaaaggt attaagatcg atgaggttgt caaaggaagc ccagctgctc aggctggctt
    93601 gcaaaaagac gatgtgatca ttggcgtcaa ccgcgatcgg gtgaactcga ttgctgaaat
    93661 gcgtaaagtg ctggcggcaa aaccggccat cattgccctg caaattgtac gcggcaatga
    93721 aagcctctac ctactaatgc gttaatgtcg taaaccgggc atcaggctga cgtgtgatgt
    93781 ccggttaact cgtggtatgc tgctgccgtt ccctttttta acgatgcctc catcatgttt
    93841 gtgaagctct tacgttccgt tgcgattgga ttaattgtcg gcgctattct gctggttgcc
    93901 atgccttcgc tgcgcagcct taacccgctt tccactccgc aatttgacag taccgatgag
    93961 acgcctgcca gctataatct ggcggttcgc cgcgccgcgc cagcggtggt taacgtttat
    94021 aaccgcggtt tgaacaccaa ctctcacaac cagcttgaga tccgcaccct gggatctggt
    94081 gtaatcatgg atcaacgcgg gtatatcatc accaataaac acgtgatcaa cgacgccgat
    94141 cagatcatcg tcgccttaca ggatggacgt gtttttgaag cgttactggt tggctctgac
    94201 tctctaaccg atctggcggt gctgaaaatt aacgccacag gcggtttacc aacgattccg
    94261 atcaatccac gccgcgtacc gcacattggc gatgtggttc tggcgatcgg taacccgtac
    94321 aacctcgggc agaccattac ccaagggatt atcagtgcca cgggtagaat cggtctgaac
    94381 ccgaccgggc ggcaaaactt cctgcaaacc gatgcttcca ttaaccacgg taactctggc
    94441 ggcgcgctgg tgaactcgct gggcgaactg atgggcatta acacgctgtc gtttgataag
    94501 agtaacgatg gcgaaacgcc ggaaggtatc ggctttgcga ttcctttcca gttagcgacc
    94561 aaaattatgg ataaactgat ccgtgatggc cgtgtgatcc gtggctacat tggcattggt
    94621 ggacgtgaga tcgccccatt acatgcgcag agtggcggca tggatcaact gcaagggatc
    94681 gtggttaacg aagtgtcgcc tgatggccca gcggcaaatg caggcatcca ggtcaacgat
    94741 ctgattatct ctgtgaataa caaacccgct atttccgccc tggagacaat ggatcaggtc
    94801 gcggaaattc gccctggttc ggtgatcccg gttgtcgtga tgcgtgacga taaacagtta
    94861 acattgcagg tcaccattca ggaatatccg gcgaccaact aaggtcagcg taaaaaaaca
    94921 aaaaccggag tctgtgctcc ggttttttat tatccgctaa tcaattactt attaacgaac
    94981 tcttcgccca gggcgatatc tttcttcagc gtatccagca taccttccag cgcgttctgt
    95041 tcaaatgcgc tcagagtacc gatagattta cgctcttcca cgccgttttt acccagcagc
    95101 agcggttgag agaagaaacg agcgtactga ccgtcgcctt caacgtaggc acattcgaca
    95161 acgccttgtt cgccctgcag tgcgcgaacc agagacagac caaaacgtgc agctgcctgg
    95221 cccatagaca gggttgcaga cccgccaccg gctttcgctt caaccacctc agtacccgcg
    95281 ttctggatac gtttggtcag atcagccact tcctgctcgg taaaactaac gccaggaacc
    95341 tgtgacagca gcggcagaat ggtaacacca gagtgaccac caataaccgg cacttcaact
    95401 tcgcctggct gtttgccttt cagttccgca acaaaggtgt tggaacgaat gatatccagc
    95461 gtggtaacgc cgaacagttt gtttttgtca taaacaccgg cttttttcag cacttcagca
    95521 gcaatcgcaa ctgtagtgtt aaccgggtta gtgataatac caatgcacgc tttcgggcag
    95581 gttttcgcaa cttcctgtac caggtttttc acgatgccgg cgttaacgtt aaacaggtcg
    95641 gaacgatcca tacccggttt acgcgctacg cctgcagaga taagaacgac atctgcacct
    95701 tccagcgccg gagtcgcatc ttcacctgaa aaacctttga ttttcacagc agtagggata
    95761 tggctcagat cgacagccac accgggagtc actggagcga tatcatacag agagagttct
    95821 gaacctgaag gcagttgggt ttttaacagt agtgcaagcg cctggccaat accgccagca
    95881 gcgccgagga ctgcgacttt catcctaaac tccttattat attgataaac taagatatat
    95941 tgctccgctg ccgcgacctt aatccacaaa attgccgttt acaatgacca catctcaaga
    96001 atgtgtagtc acgcaagttt agcgtttatg catttaattg ccgtaatcag gaacctaact
    96061 tacgtaatta acagtcactt atgcattagc gcaacattcc aacaggtggt gacaatatac
    96121 cctaccgctc agccaaaaca atatcaattt gataacaatt aatttacttt taagcagaat
    96181 ttgcaggccg tgacgcaggc atgtttctca ataacgaaat ttgataaaat cccgctcttt
    96241 cataacatta tttcagcctt cttcagggct gactgtttgc ataaaaattc atctgtatgc
    96301 acaataatgt tgtatcaacc accatatcgg gtgacttatg cgaagctcgg ctaagcaaga
    96361 agaactagtt aaagcattta aagcattact taaagaagag aaatttagct cccagggcga
    96421 aatcgtcgcc gcgttgcagg agcaaggctt tgacaatatt aatcagtcta aagtctcgcg
    96481 gatgttgacc aagtttggtg ctgtacgtac acgcaatgcc aaaatggaaa tggtttactg
    96541 cctgccagct gaactgggtg taccaaccac ctccagtcca ttgaagaatc tggtactgga
    96601 tatcgactac aacgatgcag ttgtcgtgat tcataccagc cctggtgcgg cgcagttaat
    96661 tgctcgcctg ctggactcac tgggcaaagc agaaggtatt ctgggcacca tcgctggcga
    96721 tgacaccatc tttactaccc ctgctaacgg tttcaccgtc aaagagctgt acgaagcgat
    96781 tttagagctg ttcgaccagg agctttaatc tctgccccgt cgcttctggc ggcggggaaa
    96841 atgttgcttt tgcctatcaa ccccctgctt tcccctgcga ttcatttaac gaatagtgcg
    96901 ttttactgcg acatatcatt cacacaatga atacacatag taaaaaagca cattatgcaa
    96961 aattcatt
//
LOCUS       DAZGJT010000002        96754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZGJT010000002 DAZGJT010000000
VERSION     DAZGJT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 96754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 96754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..96754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.002"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..410
                     /gene="ytcA"
                     /locus_tag="ACHKFK_000102"
     CDS             135..410
                     /gene="ytcA"
                     /locus_tag="ACHKFK_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO9050539.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPGWF
                     FCAIASLILTLITRRIIQRTNINLAFVGIIYTALFALYAMLFWLAFF"
     gene            429..1460
                     /gene="mdtN"
                     /locus_tag="ACHKFK_000103"
     CDS             429..1460
                     /gene="mdtN"
                     /locus_tag="ACHKFK_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO9050540.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQASDTLRRTEPLLREGFVSAEEVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTLATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1460..3511
                     /gene="mdtO"
                     /locus_tag="ACHKFK_000104"
     CDS             1460..3511
                     /gene="mdtO"
                     /locus_tag="ACHKFK_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO9050541.1"
                     /translation="MSALNSLPLPVVRLLTFFHEELSERRPGRVPQTMQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIASLFLIYKWSYGEP
                     LIRLIIAGPLLMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRIQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINNLQHSITEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYNPVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVVLERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGV
                     ATALSHSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            3508..4974
                     /gene="mdtP"
                     /locus_tag="ACHKFK_000105"
     CDS             3508..4974
                     /gene="mdtP"
                     /locus_tag="ACHKFK_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO9050542.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQFNDPQLDALIQRTLSGSHPLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            5172..7319
                     /gene="fdhF"
                     /locus_tag="ACHKFK_000106"
     CDS             5172..7319
                     /gene="fdhF"
                     /locus_tag="ACHKFK_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5589..5591,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase H subunit alpha,
                     selenocysteine-containing"
                     /protein_id="HFO9050543.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAESAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRVAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7694..9262
                     /locus_tag="ACHKFK_000107"
     CDS             7694..9262
                     /locus_tag="ACHKFK_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9050544.1"
                     /translation="MIKKLLPLLVLSTISATTVAATPPNTLVVAQGLDDIVSLDPAEA
                     NELSSIQTVPSLYQRVVQPDRNDPTKITPILAESWQADPAAKTLTIKLKSDAKFASGN
                     PVRPEDVIYSYVRAVTLNKSGAFILNVLGWQPENIASQLKKIDDHTVQVQWSADVSPA
                     LALNILSTPIASIVDEKLVAPNAKNNDFGNEWLKMHSAGSGAFKMRTYQPHQAIVMEA
                     NTSSPTGAPKLKSVIIKNVPDPATRRLLIQQGDADMARDLGADQIDALQGKPGVKVMS
                     IASAEQNYLAFNTGNKDNPLMSNPAFWEAARWLVDYDGITKNLLKGQYFTHQSFLPVG
                     FPGALEETPFTFNPAKAKEILAKAGIKDPHFTLDVENKPPFITIAQSIQASFAQGGVK
                     VDLLPAAGSQVYSRVRAHQHQGAIRMWLPDYFDAHSNASSFAYNDGKSSTVAGLNGWK
                     IPELNKETLAAIAEPDSAKRLDLYKKMQQELQRSSPYVFIDQGKTQIVMRDNVQGYQQ
                     GLNADMVWFDQVTK"
     gene            9362..10411
                     /locus_tag="ACHKFK_000108"
     CDS             9362..10411
                     /locus_tag="ACHKFK_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012579036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9050545.1"
                     /translation="MSVVFSTRARWRGKRPLLALLQGLFTVALTLLGLLLITFALSAL
                     SPVDRVLQIVGDHASQSTYDQVRHQLGLDQPLAVQFWHYLMNLAHGDLGIASSTVQPV
                     LQDLLSAFPATIELATLALIVGAVVGVIAGVLCARYVGSPLDFTVRTLTLLGNSVPVF
                     WLGLLMLALFYARLQWSAGPGRLDDIYQFTIEPRSGFALIDTWLSGDREAFTNAINHL
                     ILPVLLLAYFSLASITRLTRSACLGEMNKEYILLARAKGASEMTILLRHVLPNIRGTL
                     LTVIALAYTSMLEGAVLTETVFSWPGIGRYLTTALFAGDTTAIMGGTLLIGVCFVLIN
                     NLTDLLVRLTDPRLR"
     gene            10411..11244
                     /locus_tag="ACHKFK_000109"
     CDS             10411..11244
                     /locus_tag="ACHKFK_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9050546.1"
                     /translation="MPMFLFLRRLRHSPAAFCGLIIILLLMLIALFAPWLAPHDPNWQ
                     DAAARLQAPSRQHWLGTDSYGRDLLSRLIYGSRPALGLVALVTIITLPVGLLIGILAG
                     YYGGWLERVLMRFTDVVMSMPRLILAFAFVAMLGPGLVNGALALALTTWPAYARQARS
                     EIQRLRHSDYLAAAEMMGIRGRRLLAGHILPLCLPSAIVRLALDLAGIILAAAGLGFL
                     GLGARPPMAEWGAMIADGMQVIFDQWWIAAAPGAIILIASLAFNLLGDGLRDVLEPQH
                     D"
     gene            11237..12073
                     /locus_tag="ACHKFK_000110"
     CDS             11237..12073
                     /locus_tag="ACHKFK_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001517582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9050547.1"
                     /translation="MTDSRLSINNLCVDYPESRVVNNVSFTLGNERLALVGESGSGKS
                     MTARALMGLVRKPGVVTADTLNILGRDALTLNARGWQQLRGNDIAMVLQDPRYALNPV
                     KTVKAQLEEALTLHQRLNRREKEEKINTAIQAVGLNTRVLQSYPRELSGGMGQRVMIA
                     IALINDPRVLIADEPTSALDVRLRNQILELLVTQCEQRQMAMLLISHDLPLVAQFCHR
                     VLVMYQGNKVDEMHAAALPTATHPYTRTLWTCRPNAQTYGQMLPTLDRTAMTPEKYHD
                     DC"
     gene            12060..12764
                     /locus_tag="ACHKFK_000111"
     CDS             12060..12764
                     /locus_tag="ACHKFK_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9050548.1"
                     /translation="MTIVEINHLQVTFAEKTAVSAASFSVNAGETFSLIGESGCEKST
                     ILRVLAGLQRDWHGQVSLFGHTIKPGMRFQGDLRRNVQMVFQDPYASLHPNHTLWRTL
                     AEPLKIRGEREIEKRVQTALEQVGLPFDAARRYPHQLSGGQRQRVVIARALLLRPQLL
                     LLDEPTSALDMSVQAEILNLLNQLKLEHQMTYLLVSHDADVIAHMSDRAALMSEGKIQ
                     RFFDRDAMEKGEHRMD"
     gene            12875..13564
                     /gene="yjcO"
                     /locus_tag="ACHKFK_000112"
     CDS             12875..13564
                     /gene="yjcO"
                     /locus_tag="ACHKFK_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO9050549.1"
                     /translation="MKKIITLMLFLTFFAHANDSEPGSQYLKAAEARDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13937..15250)
                     /gene="gltP"
                     /locus_tag="ACHKFK_000113"
     CDS             complement(13937..15250)
                     /gene="gltP"
                     /locus_tag="ACHKFK_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO9050550.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(15593..16189)
                     /gene="nrfG"
                     /locus_tag="ACHKFK_000114"
     CDS             complement(15593..16189)
                     /gene="nrfG"
                     /locus_tag="ACHKFK_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO9050551.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTVQARAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(16186..16569)
                     /gene="nrfF"
                     /locus_tag="ACHKFK_000115"
     CDS             complement(16186..16569)
                     /gene="nrfF"
                     /locus_tag="ACHKFK_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO9050552.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQEQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYTMVEEGKSEVEIIDWMTERYGDFVRYNPPLTGQT
                     LVLWALSVVLLLLMALILWRVRAKR"
     gene            complement(16562..18280)
                     /gene="nrfE"
                     /locus_tag="ACHKFK_000116"
     CDS             complement(16562..18280)
                     /gene="nrfE"
                     /locus_tag="ACHKFK_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO9050553.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLATLLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRSEFDGVSARICWRWARPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFALLMLVVIVLATFVSGKRAQLPALLAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            complement(18300..19256)
                     /gene="nrfD"
                     /locus_tag="ACHKFK_000117"
     CDS             complement(18300..19256)
                     /gene="nrfD"
                     /locus_tag="ACHKFK_000117"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO9050554.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVRKVLSLLTPVHRGLETLMLVLAALLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(19253..19924)
                     /gene="nrfC"
                     /locus_tag="ACHKFK_000118"
     CDS             complement(19253..19924)
                     /gene="nrfC"
                     /locus_tag="ACHKFK_000118"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO9050555.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACRKVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTHL
                     QAGKLPACVEACPTKALTFGNLDDPSSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(19921..20487)
                     /gene="nrfB"
                     /locus_tag="ACHKFK_000119"
     CDS             complement(19921..20487)
                     /gene="nrfB"
                     /locus_tag="ACHKFK_000119"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO9050556.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(20532..21968)
                     /gene="nrfA"
                     /locus_tag="ACHKFK_000120"
     CDS             complement(20532..21968)
                     /gene="nrfA"
                     /locus_tag="ACHKFK_000120"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO9050557.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPATPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            22360..24318
                     /gene="acs"
                     /locus_tag="ACHKFK_000121"
     CDS             22360..24318
                     /gene="acs"
                     /locus_tag="ACHKFK_000121"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO9050558.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINAPDTFWGEQGK
                     ILDWITPYQKVKNTSFAPGNVSIQWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSSSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHNLVEQASAQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            24530..24844
                     /gene="yjcH"
                     /locus_tag="ACHKFK_000122"
     CDS             24530..24844
                     /gene="yjcH"
                     /locus_tag="ACHKFK_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO9050559.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPVGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            24841..26490
                     /gene="actP"
                     /locus_tag="ACHKFK_000123"
     CDS             24841..26490
                     /gene="actP"
                     /locus_tag="ACHKFK_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO9050560.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRNDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(26529..27218)
                     /locus_tag="ACHKFK_000124"
     CDS             complement(26529..27218)
                     /locus_tag="ACHKFK_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFO9050561.1"
                     /translation="MANFQLSMLCLAITLVIYFLNKRLYRRFRKLPLMPLVLTPTLLV
                     MLLVFGNISWQNYIGEAHWLLWLLGPATIAFAVPVYDNRAIIKRHWMSLTAGVLTATV
                     VAVTSSVWLARLFTLPDEIQRSLAVRSVTTPFALAASQSLGGQPDLVALFVVVTGVFG
                     MAIGDVLFLRLSIREGMAKGAGFGAASHGAGTARSYELGQQEGVVASLVMMLSGVLMV
                     LVSPLVARMMF"
     gene            complement(27211..27621)
                     /locus_tag="ACHKFK_000125"
     CDS             complement(27211..27621)
                     /locus_tag="ACHKFK_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001143718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO9050562.1"
                     /translation="MPVALRRITPAVVQSIQVFFQVVLYAGLFIFAQYLVSWLHLPLP
                     ANLVGMILMLALIVCRILPLQWVRAGARWLLAEMLLFFVPAVVAVVNYAQLLLVDGWR
                     IFAVIALSTVMVLGTTAWVVEKVYRYEMRRLNRG"
     gene            27725..28609
                     /locus_tag="ACHKFK_000126"
     CDS             27725..28609
                     /locus_tag="ACHKFK_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9050563.1"
                     /translation="MDIRTLRYFVEVVRQQSFTRAAEKLFVTQPTISKMLKNLEDELN
                     CILLIRDGRRLLLTDTGRVVFERGLALLAEFRQLEAELSDINQLKTGVLRLGIPPMVG
                     MLMAGPIGLFRQRYPGVELKISEFGGLTVQQAVMNGELDMALTALPVEESGLTTLSLF
                     SHPLCVLTPHSGKWLECDAVSPETLAEHPLLIYNEDFALSKQLMKLFDEHGVKPRIAV
                     RSGQWDFLAAMVQAGVGIAILPEPICQRLDKNTLKWLPLESELSWKLGMIWREGVYLS
                     HSAHAWLSCCEGYWLTPE"
     gene            complement(28645..30294)
                     /gene="yjcE"
                     /locus_tag="ACHKFK_000127"
     CDS             complement(28645..30294)
                     /gene="yjcE"
                     /locus_tag="ACHKFK_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9050564.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSILEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(30445..31794)
                     /gene="ghxP"
                     /locus_tag="ACHKFK_000128"
                     /gene_synonym="yjcD"
     CDS             complement(30445..31794)
                     /gene="ghxP"
                     /locus_tag="ACHKFK_000128"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO9050565.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFTTFPVMMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(32341..32805)
                     /gene="soxR"
                     /locus_tag="ACHKFK_000129"
     CDS             complement(32341..32805)
                     /gene="soxR"
                     /locus_tag="ACHKFK_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO9050566.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIREAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            32891..33214
                     /gene="soxS"
                     /locus_tag="ACHKFK_000130"
     CDS             32891..33214
                     /gene="soxS"
                     /locus_tag="ACHKFK_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO9050567.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(33250..34803)
                     /gene="pdeC"
                     /locus_tag="ACHKFK_000131"
     CDS             complement(33250..34803)
                     /gene="pdeC"
                     /locus_tag="ACHKFK_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO9050568.1"
                     /translation="MSHRARHQLLAFPGIIFLVLFPIILSLWIAFFWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIASVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSNEASAATFSPLIHLNDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQGQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGAYLATHTDRYVSINLSASDFHTSRLIARTNQKTEQHAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRKAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEAQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMG"
     gene            35233..35514
                     /gene="yjcB"
                     /locus_tag="ACHKFK_000132"
     CDS             35233..35514
                     /gene="yjcB"
                     /locus_tag="ACHKFK_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO9050569.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRADYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(35613..36149)
                     /gene="ssb1"
                     /locus_tag="ACHKFK_000133"
     CDS             complement(35613..36149)
                     /gene="ssb1"
                     /locus_tag="ACHKFK_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO9050570.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            36404..39226
                     /gene="uvrA"
                     /locus_tag="ACHKFK_000134"
     CDS             36404..39226
                     /gene="uvrA"
                     /locus_tag="ACHKFK_000134"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO9050571.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLYARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(39435..39791)
                     /gene="yjbR"
                     /locus_tag="ACHKFK_000135"
     CDS             complement(39435..39791)
                     /gene="yjbR"
                     /locus_tag="ACHKFK_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9050572.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(39795..40211)
                     /gene="yjbQ"
                     /locus_tag="ACHKFK_000136"
     CDS             complement(39795..40211)
                     /gene="yjbQ"
                     /locus_tag="ACHKFK_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO9050573.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRAVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(40322..41035)
                     /gene="aphA"
                     /locus_tag="ACHKFK_000137"
     CDS             complement(40322..41035)
                     /gene="aphA"
                     /locus_tag="ACHKFK_000137"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO9050574.1"
                     /translation="MRKITQALSAVCLLFALNSSAVALASSPSPLNPGTNVAKLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKLGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            41312..43132
                     /locus_tag="ACHKFK_000138"
     CDS             41312..43132
                     /locus_tag="ACHKFK_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9050575.1"
                     /translation="MKISWNYIFKNKWRFHITSISLFLIMLAVSIAFLHLRFNTLSGT
                     DKMRLEMYKSTLYSTIEQFYVLPYMLSTDHIIRQAVITPDDMTSSELNQRIAHFNTQL
                     KTAAIFILDTQGKAIASSNWQDPGSYVGQNYSYRPYYKHAMSGLNGRFYGIGSTTNTP
                     GFFLSTSIKDKGKIVGVVVVKISLNEIEKAWAEGPENIIVNDEHGIIFLSSKSPWRMR
                     TLQPLPVQAKQKLQSTRQYSLDNLLPADYYPCYTVSNFTFLKDKKEQLCLFPQYYTQQ
                     IAIPEFNWKMTIMVPLDNLYWSWAISLVITLIIYLLFLLFIKYWRMRSHAQQLLTLAN
                     ETLEKQVKERTSALELINQKLIQEIKERSQAEQVLQITRSELAESSKLAALGQMATEI
                     AHEQNQPLAAIHALTDNARTMLKKEMYPQVEQNLKHIISVIERMTQLISELKAFASRH
                     RVPKGSADVIKVMYSAVALLNHSMEKNNIERRIKAPSMPLFVNCDELGLEQIFSNLIS
                     NALDSMEGSSYKRLDIAIRQANNKVIITIKDSGGGFAPEVVDRIFEPFFTTKRRGMGL
                     GLAIVSEIVRNSNGALHASNHPEGGAVMTLTWPEWGEEHE"
     gene            43125..44483
                     /locus_tag="ACHKFK_000139"
     CDS             43125..44483
                     /locus_tag="ACHKFK_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFO9050576.1"
                     /translation="MNNKSSEITIVYIEDSDDVRFACEQTLTLAGYRVISCCDAEHSI
                     PLIQSQANIIILTDVRLPGISGLELLSYINEMDSKIPVILITGHGDVEMAVDAMRNGA
                     FDFIEKPSSSDKLLSIIARAVEKRRLVLENQQLLANLQQENGPVLIGRSPQMQQLRKM
                     ILNVADTGADVLIYGETGCGKEVVARMLHHWSTRRQGQFVALNCAGLPETLFESEIFG
                     HEAGAFTGAVKKRIGKIEHANGGTLFLDEIEGMPSGMQVKLLRVLQERTIERLGANQL
                     IPVNCRVIAATKEDLLRRSEEHLFRLDLYYRLNVVSLNIPPLRQRREDIPELFYWFAS
                     QAAQKYNRPLPDISPMLLAWLQSQSWPGNVRELKHNAERFVLGLLTHHQPVPMTQQEE
                     SGLTACIDAFEKKLIEDMLRQTEGQVSLTARLLQLPRKTLYDKLNKHQIQPQVYRPES
                     SS"
     gene            complement(44472..45545)
                     /locus_tag="ACHKFK_000140"
     CDS             complement(44472..45545)
                     /locus_tag="ACHKFK_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HFO9050577.1"
                     /translation="MSLQIAVEKVRWLAAGLLELNGCDADIAQDVAEHMIEAERYGFA
                     SHGVTLLPKYLENIARGDVTANARPECLTSEGNLQRFHAHNGFGQHAGKVAVNAAIEQ
                     AKHRGFCLLTLCHAHHLGRMGHYGQMVADQGLAMLAMSNVTGRPALVAPWGGAEPRMT
                     TNPFCFAWPFSDGRPPILVDFATSSMALNKARVMSSTGKQAAPGQLIDAQGNPSNDPG
                     VLFSNPPGALLPFGEHKGFGLALMIEMMAGILSGGDTIAEDHQTDGSAHNHLFALIID
                     PDQFTDLAGEKGQFFADYLLATQPQPGGNPVIYPGMPEAANRERNAKMITIPVSFWSW
                     VVEHYARKGIDLGLHPQESALAG"
     gene            complement(45557..47056)
                     /locus_tag="ACHKFK_000141"
     CDS             complement(45557..47056)
                     /locus_tag="ACHKFK_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFO9050578.1"
                     /translation="MSSISHGAPQKRRIIPNPGLWLAIIAGIIITLLPLGDTLPVAGQ
                     NMIAILVFAIIVWISEAMDYTASAIVISALIIFMVGFAPDMNHPDTILGTAKALKMTL
                     SGFSNSALALVAAAMFIAAAMTITGLDKRIALFTMSKIGASSRSIIIGAIVVTIVLSL
                     VVPSATARTACVVPIMMGVIAAFKVDKHSRLAASMMIVIAQATSIWNVGIQTSAAQNL
                     LSIGFINKTFGAGHSVSWLDWLLAGAPWSLTMSAILYFLARKLLPPETEAVEGGSEAI
                     KKALAELGPTTGKEKRLIGISLLLLLFWSTGGKLHSIDTTSVTLAGLAIMLLPGIGVM
                     SWKEVEKRVQWGTLLMFGIGISLGSTLLDTQAASWMANYVVKGFGLDGLPSLAIFAIL
                     AAFLIIIHLGFASATALTAALLPILISLLSSLPPELGVNPVGMTILLAFSVSFGFILP
                     INAPQNMVCMGTDTFTPRQFTRVGLYLTVIGYLLLLLFAATWWKILGLM"
     gene            complement(47268..48135)
                     /gene="sucD"
                     /locus_tag="ACHKFK_000142"
                     /pseudo
     CDS             complement(47268..48135)
                     /gene="sucD"
                     /locus_tag="ACHKFK_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858609.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
     gene            complement(48148..49317)
                     /gene="sucC"
                     /locus_tag="ACHKFK_000143"
     CDS             complement(48148..49317)
                     /gene="sucC"
                     /locus_tag="ACHKFK_000143"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO9050579.1"
                     /translation="MNLHEYQAKSLLAGMGMPCPKEIAIQQISQLADAWQHIACPSKG
                     AVLKAQVHAGGRGKAGGVKVLKQLPEAQAFVQQMLGSQLVTYQTGPEGQYVSSILLCE
                     NIYPVRQELYFGMVVDRESQRVTFIVSPEGGVEIEKVAHETPEKISSVSIDPLTGVQP
                     CHIREMFAVLQLEHGLFAAFSRLVNQAWKAFNELDFALLEINPLVLRETGEFMCADAK
                     VSLDDNALYRHPELQVLRDETQEDPRESQAAKLDLNYVSLDGNIGCMVNGAGLAMATM
                     DIIKLYGEQPANFLDVGGGATQERVSEAFRLIVSDSKVKAILVNIFGGIVRCDMIARA
                     IIHALNEARITLPVVVRLSGNNAAEGQRLLAESGLTVEAVNSLDDAAKRIIALLN"
     gene            complement(49339..50757)
                     /gene="lpdA"
                     /locus_tag="ACHKFK_000144"
     CDS             complement(49339..50757)
                     /gene="lpdA"
                     /locus_tag="ACHKFK_000144"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO9050580.1"
                     /translation="MSTIFDVAVMGGGPGGYVAALRAAQNGLSVVCIDDGVNAQGEPS
                     PGGTCLNVGCIPSKSLLQSSELYAQVQHEASIHGVNVDGVSFNAAAMIQRKDAIVSRL
                     TMGIGLLFKKNKVKYLCGLATLEHARDEIWQLRVNDQQIRARNVVIATGSQPRQLPGV
                     TIDNQQILDNRGALALTAVPPRLGVIGAGVIGLELGSVWNRVGSDVTLLEMAPTFLPA
                     LEARLSNEVRKAMIASGMKMQLAVEIEAIEQRDDGVHVRWRQGEKREESRFDKLILAI
                     GRVPRLSGVDLVQLGLEADNRGGIAVDNLCRTGKAGLWAIGDVVRGPMLAHKAMAEGV
                     VVADQIAGLAVEPINFALIPSVIYTQPEVAWVGENEASLKAAGRVFNKGNSLFAGNGR
                     ALALGQEGGRCTLYSDKHTDRVLGGAIVGPQASELINEIALAMTFSASGEDIACAIHA
                     HPTLSEVIHEAAMALNNKALHG"
     gene            complement(50769..51915)
                     /gene="sucB"
                     /locus_tag="ACHKFK_000145"
                     /pseudo
     CDS             complement(50769..51915)
                     /gene="sucB"
                     /locus_tag="ACHKFK_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468318.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
     gene            complement(51948..54692)
                     /locus_tag="ACHKFK_000146"
     CDS             complement(51948..54692)
                     /locus_tag="ACHKFK_000146"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO9050581.1"
                     /translation="MENMTSPGTLLSGDNATWLEDYYQTWLRTPEQLPEDWRRFFLSP
                     ELTVQSVSGDNNVSGATLKKQAAIIQLINAWRTQGHLRAKLDPLGLNPPADVPSLLPG
                     FWGLSEEDLLQEFSVTFGAHTTQMPLKQLLNLLEQAWAGSQAYELAHLENREEINWLL
                     SRIESSNAPQADVQTCIARFEKLMAAETLERYLHTRYVGQKRFSLEGGESAIPALDTL
                     TKRLRAQGVEEMVIGMAHRGRLNVLVNLLNKDPAQLFAEFEGKQTIGSGSGDVKYHMG
                     YSSNLETPAGSLHVALAYNPSHLEIVNPVVLGQVRARQERRGEDGQAKVVGVLIHGDS
                     ALGGLGVNQTTFNLSQTQGYGTGGTLHLVINNQIGFTTSRLQDMRSSRYCTDIAKMVA
                     APIIHVNGDDVDAVCQVMELACEWRDTFRRDIIIDICCFRKHGHNESDEPRLTQPQMY
                     QAVDAHPGTLARYGESLARRGLLTQAQQDEMTARYRDWLDSCQKREPQPLKPAIHSFS
                     ANWYGLTNPHWSAPVSTALPRQKLAAYGEIISTLPPDVVAHPTIKRQLALRQDMAAGT
                     QPIDWGMAEMLAYASLVDAGVGVRLSGEDSGRGTFSHRHAVVHHQTEARRYLPLQHIR
                     AGQASFDVYDSVLNEEALLAFEYGYSTSAPQQLVIWEAQFGDFANGAQVAIDQFISSG
                     ETKWDRYSGLTILLPHGYDGQGPEHSSARPERWLQLCAENNMQVVMPSESAQMFHLLR
                     GQALRPMRKPLVIMMSKRLLRFKGAMSELCEFTDGAYKPVITDPQLHQSQKVKRVILC
                     SGQVYYDVLEARKQREHEDEVAIVRLEQLYPFPVAELNDVLASWPNCCEWIWLQEEPE
                     NQGAWRQIRHELAALKINTPYWQYAGRPAAAAPATGYGRVHKQQIDEFLAAAFADIQP
                     "
     gene            complement(54874..56067)
                     /gene="tyrB"
                     /locus_tag="ACHKFK_000147"
     CDS             complement(54874..56067)
                     /gene="tyrB"
                     /locus_tag="ACHKFK_000147"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO9050582.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVADAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDSVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAKVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLRTAQVDRLREEFGVYLIASGRMCVAGLNAQNVHRVAKA
                     FAAVM"
     gene            complement(56320..57399)
                     /gene="alr"
                     /locus_tag="ACHKFK_000148"
     CDS             complement(56320..57399)
                     /gene="alr"
                     /locus_tag="ACHKFK_000148"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO9050583.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(57452..58867)
                     /gene="dnaB"
                     /locus_tag="ACHKFK_000149"
     CDS             complement(57452..58867)
                     /gene="dnaB"
                     /locus_tag="ACHKFK_000149"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO9050584.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            58950..59933
                     /gene="qorA"
                     /locus_tag="ACHKFK_000150"
     CDS             58950..59933
                     /gene="qorA"
                     /locus_tag="ACHKFK_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO9050585.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDARRAHEILESRATQGSSLLIP"
     gene            complement(60098..60340)
                     /gene="pspG"
                     /locus_tag="ACHKFK_000151"
     CDS             complement(60098..60340)
                     /gene="pspG"
                     /locus_tag="ACHKFK_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO9050586.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALIVATAFMFLGGMLALM
                     IKLLPWLLLAVAVVWVIRAIKAPKVPKYQRYDRWRY"
     gene            complement(60474..61469)
                     /gene="dusA"
                     /locus_tag="ACHKFK_000152"
     CDS             complement(60474..61469)
                     /gene="dusA"
                     /locus_tag="ACHKFK_000152"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO9050587.1"
                     /translation="MPAESHTVYPAYRFSIAPMLDWTDRHCRYFLRLLSRNTLLYTEM
                     VTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPS
                     DRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTV
                     SGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSL
                     EEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEREL
                     SQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR"
     gene            61625..62140
                     /gene="zur"
                     /locus_tag="ACHKFK_000153"
     CDS             61625..62140
                     /gene="zur"
                     /locus_tag="ACHKFK_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO9050588.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALEFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCSACVEVEACRHPEQCHH
                     DHSIQVKKKPR"
     gene            complement(62182..62391)
                     /gene="yjbJ"
                     /locus_tag="ACHKFK_000154"
     CDS             complement(62182..62391)
                     /gene="yjbJ"
                     /locus_tag="ACHKFK_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO9050589.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVDSWEKRHDYRW"
     gene            complement(62507..63832)
                     /gene="dinF"
                     /locus_tag="ACHKFK_000155"
     CDS             complement(62507..63832)
                     /gene="dinF"
                     /locus_tag="ACHKFK_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO9050590.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRHLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNDTWFVAT"
     gene            complement(63905..64513)
                     /gene="lexA"
                     /locus_tag="ACHKFK_000156"
     CDS             complement(63905..64513)
                     /gene="lexA"
                     /locus_tag="ACHKFK_000156"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO9050591.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(64623..64991)
                     /gene="dgkA"
                     /locus_tag="ACHKFK_000157"
     CDS             complement(64623..64991)
                     /gene="dgkA"
                     /locus_tag="ACHKFK_000157"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO9050592.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            65162..67585
                     /gene="plsB"
                     /locus_tag="ACHKFK_000158"
     CDS             65162..67585
                     /gene="plsB"
                     /locus_tag="ACHKFK_000158"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO9050593.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(67740..68612)
                     /gene="ubiA"
                     /locus_tag="ACHKFK_000159"
     CDS             complement(67740..68612)
                     /gene="ubiA"
                     /locus_tag="ACHKFK_000159"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO9050594.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(68625..69122)
                     /gene="ubiC"
                     /locus_tag="ACHKFK_000160"
     CDS             complement(68625..69122)
                     /gene="ubiC"
                     /locus_tag="ACHKFK_000160"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO9050595.1"
                     /translation="MSHPALTQLRALRYFTEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(69301..70221)
                     /gene="malM"
                     /locus_tag="ACHKFK_000161"
     CDS             complement(69301..70221)
                     /gene="malM"
                     /locus_tag="ACHKFK_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO9050596.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLSTGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(70635..71975)
                     /gene="lamB"
                     /locus_tag="ACHKFK_000162"
     CDS             complement(70635..71975)
                     /gene="lamB"
                     /locus_tag="ACHKFK_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO9050597.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGSSSTNPYYGKAVSADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(72047..73162)
                     /gene="malK"
                     /locus_tag="ACHKFK_000163"
     CDS             complement(72047..73162)
                     /gene="malK"
                     /locus_tag="ACHKFK_000163"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO9050598.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLEMITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            73527..74717
                     /gene="malE"
                     /locus_tag="ACHKFK_000164"
     CDS             73527..74717
                     /gene="malE"
                     /locus_tag="ACHKFK_000164"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO9050599.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYQ
                     DELAKDPRIAATMDNAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDAALKDAQT
                     RITK"
     gene            74871..76415
                     /gene="malF"
                     /locus_tag="ACHKFK_000165"
     CDS             74871..76415
                     /gene="malF"
                     /locus_tag="ACHKFK_000165"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO9050600.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKIYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ESATQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            76430..77320
                     /gene="malG"
                     /locus_tag="ACHKFK_000166"
     CDS             76430..77320
                     /gene="malG"
                     /locus_tag="ACHKFK_000166"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO9050601.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(77414..77824)
                     /gene="psiE"
                     /locus_tag="ACHKFK_000167"
     CDS             complement(77414..77824)
                     /gene="psiE"
                     /locus_tag="ACHKFK_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO9050602.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFVPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            78038..78316
                     /locus_tag="ACHKFK_000168"
     CDS             78038..78316
                     /locus_tag="ACHKFK_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050603.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGLLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGY"
     gene            complement(78363..80459)
                     /gene="yjbH"
                     /locus_tag="ACHKFK_000169"
     CDS             complement(78363..80459)
                     /gene="yjbH"
                     /locus_tag="ACHKFK_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO9050604.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPKYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLGGEP
                     LGHETTLAQKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGVGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(80459..81196)
                     /gene="yjbG"
                     /locus_tag="ACHKFK_000170"
     CDS             complement(80459..81196)
                     /gene="yjbG"
                     /locus_tag="ACHKFK_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO9050605.1"
                     /translation="MIKQTIVALLLSAGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGTPPSTVTLFGLISRPGKQS
                     FTLGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(81193..81830)
                     /gene="yjbF"
                     /locus_tag="ACHKFK_000171"
                     /pseudo
     CDS             complement(81193..81830)
                     /gene="yjbF"
                     /locus_tag="ACHKFK_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
     gene            complement(82017..82259)
                     /gene="yjbE"
                     /locus_tag="ACHKFK_000172"
     CDS             complement(82017..82259)
                     /gene="yjbE"
                     /locus_tag="ACHKFK_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO9050606.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(82757..84406)
                     /gene="pgi"
                     /locus_tag="ACHKFK_000173"
     CDS             complement(82757..84406)
                     /gene="pgi"
                     /locus_tag="ACHKFK_000173"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO9050607.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGKVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      84643..84839
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            84931..86280
                     /gene="lysC"
                     /locus_tag="ACHKFK_000174"
     CDS             84931..86280
                     /gene="lysC"
                     /locus_tag="ACHKFK_000174"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO9050608.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            86533..86805
                     /gene="yjbD"
                     /locus_tag="ACHKFK_000175"
     CDS             86533..86805
                     /gene="yjbD"
                     /locus_tag="ACHKFK_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO9050609.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(86806..87678)
                     /gene="rluF"
                     /locus_tag="ACHKFK_000176"
     CDS             complement(86806..87678)
                     /gene="rluF"
                     /locus_tag="ACHKFK_000176"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO9050610.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQCNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPRESEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTVGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            88026..88973
                     /locus_tag="ACHKFK_000177"
     CDS             88026..88973
                     /locus_tag="ACHKFK_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFO9050611.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGIVTIAINYDYNENLWLEQQVKQKFGLKDVVVVSGNDEDEDTQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDVALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDIFSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIVGALRGKYINCLVTNSSTAELLLK"
     gene            89043..89849
                     /locus_tag="ACHKFK_000178"
     CDS             89043..89849
                     /locus_tag="ACHKFK_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9050612.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVDELLAQGANVQMVDIHCG
                     DGQYESHKGYQFWPTDISSAKEINHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            89859..90266
                     /locus_tag="ACHKFK_000179"
     CDS             89859..90266
                     /locus_tag="ACHKFK_000179"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose/fructose/sorbose PTS transporter subunit
                     IIA"
                     /protein_id="HFO9050613.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLDKLVSTHNHDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNIDELCEHLTQIAKQTCVVWRQVATAEEDF"
     gene            90266..90760
                     /locus_tag="ACHKFK_000180"
     CDS             90266..90760
                     /locus_tag="ACHKFK_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFO9050614.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            90826..91623
                     /locus_tag="ACHKFK_000181"
     CDS             90826..91623
                     /locus_tag="ACHKFK_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HFO9050615.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIMLGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALLYIQLNPQWRKAEPHPQTTTITALDQLDD"
     gene            91634..92458
                     /locus_tag="ACHKFK_000182"
     CDS             91634..92458
                     /locus_tag="ACHKFK_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HFO9050616.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNLLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTPAADGAT
                     VTITVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            92510..93739
                     /gene="sorE"
                     /locus_tag="ACHKFK_000183"
     CDS             92510..93739
                     /gene="sorE"
                     /locus_tag="ACHKFK_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HFO9050617.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEHDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAIGGGKKLVYTGKYLPLTSLTQIQDQALAVILAHHQGIWS
                     GEAEQYLLAHAEAISHD"
     gene            94120..94809
                     /gene="pepE"
                     /locus_tag="ACHKFK_000184"
     CDS             94120..94809
                     /gene="pepE"
                     /locus_tag="ACHKFK_000184"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO9050618.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(94899..96530)
                     /gene="yjbB"
                     /locus_tag="ACHKFK_000185"
     CDS             complement(94899..96530)
                     /gene="yjbB"
                     /locus_tag="ACHKFK_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO9050619.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLPLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
BASE COUNT        23772 a        25752 c        24504 g        22726 t
ORIGIN      
        1 aaaatatata agttatgaat gcggtggctg aataaagcta tttttattca tagggaaaat
       61 agccatactg acgccttcaa tggatattga tattcactac agatattgtt cgcagcagac
      121 aggatgtatt taatatgcca acagttctct ctcgcatggc gatgcaactc aaaaaaacag
      181 cctggataat tcccgtcttc atggtttcgg gatgctcatt atctccggca atcccggtga
      241 tcggcgctta ttatcccggc tggtttttct gcgctattgc cagccttatt ttgacgctca
      301 tcacgaggcg aattattcag cggacaaata tcaatctggc atttgtcgga attatttata
      361 ccgccctttt tgctctctac gccatgctgt tctggctggc atttttctaa tctatttgag
      421 acgcgctcat ggaaagtacg ccgaaaaaag ctcctcgcag taaattccct gctctgttag
      481 tggttgcgtt ggcgctggtt gcccttgttt tcgttatctg gcgcgtagac agtgcgccat
      541 caactaatga cgcttacgcg tcagcagata ccattgatgt ggtgccggaa gtcagcggtc
      601 gcattgtaga actggcggtc accgacaacc aggcggtgaa acagggcgat ttgctgttcc
      661 gcatcgaccc gcgcccgtac gaagccaatc tggcgaaagc cgaagcctcc ctcgcggcgc
      721 tggataagca aattatgctc acccagcgta gcgttgacgc acaacagttt ggtgccgact
      781 cggttaatgc cacggtagaa aaagcccgtg ccgccgcgaa acaagccagc gatacgttac
      841 gccgtaccga accgctactg cgagaaggtt ttgtttctgc ggaagaggtt gaccgtgcaa
      901 gaaccgcaca gcgtgcggca gaagcggacc ttaatgccgt attgttgcag gcgcagtcag
      961 ccgccagcgc cgtcagcggc gtggatgcgc tggtggcgca gcgtgcggca gtggaagcgg
     1021 atattgccct gaccaaactg catctggaaa tggcgaccgt tcgcgcgccg tttgatggcc
     1081 gggtcatttc cctcaaaacc tccgtcgggc agtttgcatc tgcgatgcgc cctattttca
     1141 ctctaatcga cactcgtcac tggtacgtga ttgccaactt ccgcgaaact gatctgaaaa
     1201 atattcgctc gggtacactc gcaaccattc gcctgatgag cgacagcggc aaaaccttcg
     1261 agggtaaagt ggattcgatt ggctacggcg tgctaccgga tgacggcggc ctggtgctgg
     1321 gcggcctgcc gaaagtgtct cgttctatta actgggtccg cgttgcccag cgttttccgg
     1381 tcaaaatcat ggttgataaa cctgacccgg aaatgttccg catcggcgct tcggcagtcg
     1441 ctaatcttga gccgcaataa tgagcgcgct caactccctg ccattaccgg tggtcaggct
     1501 gctgacgttc tttcatgaag agttaagcga gcggcgacca gggcgcgtgc cacagacgat
     1561 gcaactctgg gtaggttgcc tgctggtgat tctgatctcg atgacttttg agatcccttt
     1621 tgtggcgtta tcactggcag tgctgtttta cggtattcag tcgaacgcgt tttacaccaa
     1681 atttgtggcg atcttgtttg tggtcgccac ggtgctggag atcgccagcc tgtttttgat
     1741 ctacaaatgg tcatatggcg aaccgttgat ccgattgatc atcgccgggc cgctcctgat
     1801 gggctgcatg tttttgatgc gcacccatcg gctgggactg gtctttttcg ccgtcgccat
     1861 tgtcgctatt tacgggcaaa ccttccccgc catgctcgac tatccggaag tggtcgtgcg
     1921 cttaacgctg tggtgtatcg ttgttggcct ctatccaacc ttgctgatga cgttaatcgg
     1981 cgtgctgtgg tttcccagtc gtgccattac gcagatgcat caggcgctta atgatcggct
     2041 tgatgatgcc attagccacc tgacggacag cctcgcaccg ctacccgaaa cgcggattga
     2101 aagagaggcg ctggcgctgc aaaaactcaa tgtcttttgc ctcgcggacg atgccaactg
     2161 gcgaatccag agcgcatggt ggcaaagctg cgtggcaacg gtaacctaca tttactcgac
     2221 gctaaatcgc tacgatccca cctcttttgc tgattctcag gcaattattg aattccgaca
     2281 aaaattagct tcagaaatca acaatctgca gcattccatt accgaaggtc agtgctggca
     2341 aagcgactgg cggatcactg aaagtgaagc gatggcggca cgggaatgta acctggagaa
     2401 tatctgccag acgctgttac agctgggtca gatggacccg aatacgccgc caacgcccgc
     2461 agccaaaccg ccatcaatgg tcgccgatgc ttttaccaat ccagactata tgcgctacgc
     2521 ggtaaaaacg ctgctcgcct gtttgatctg ttacaccttc tacagcggcg tggactggga
     2581 aggcattcac acctgtatgc tgacctgcgt gatcgtcgct aatccaaatg tcggttcgtc
     2641 gtaccagaag atggtgctgc gttttggtgg ggccttttgc ggcgcgattc tggcgctgtt
     2701 attcacgcta ctggtcatgc cctggctgga caatattgtc gaattgctgt ttgtgctggc
     2761 accgattttc ctgttgggcg catggattgc caccagctct gaacgctctt cttatatcgg
     2821 cacacagatg gtggtcacct tcgcgctcgc cacgctcgaa aacgtttttg gcccggtgta
     2881 caacccggtg gaaattcgcg atcgcgctct gggtatcatc attggtaccg tggtgtcggc
     2941 ggtgatttac acctttgtct ggcctgaaag cgaagcgcgc acgctgccgc aaaaactggc
     3001 tggcgcgctg ggtatgttga gtaaagtaat gcggatccca cgccagcagg aagtcacggc
     3061 tctgcgcact tatctgcaaa ttcgcatagg tctgcatgcg gcgtttaatg cctgtgaaga
     3121 gatgtgccaa cgcgtggtgc tggagcgtca actggacagc gaagaacgcg cattactgat
     3181 tgaacgttcg caaacggtta ttcgtcaggg ccgcgatatt cttcacgcct gggatgccac
     3241 ctggaactcg gcgcaggcgc tggataacgc actacagccg gacagagcag gtcagtttgc
     3301 cgatgccctg gagaaatacg ccgccggtgt ggctaccgca ctcagtcatt ctcctcaaat
     3361 aacgcttgaa gaaacacccg cctcgcaggc catcctgccg accttattaa aacaggagca
     3421 acacgtctgc cagcttttcg cccgcttgcc agactggaca gccccggcat taacgcccgc
     3481 cacggaacag gcacaaggag ccacgcaatg atcaatcgtc aactttcacg cctgctgttg
     3541 tgcagcattc tcggcagcac gacgctgatt tccggctgtg ccttggtacg taaggattct
     3601 gcgcctcatc aacagctcaa accggaacaa atcaaactgg ccgatgatat tcatcttgcc
     3661 agctccggct ggccgcaggc gcagtggtgg aaacaattta acgacccgca gctggatgcg
     3721 ctgatccaac ggacgctaag tggttcacac cccctcgccg aagcgaaact gcgggaagaa
     3781 aaagcgcagt cccaggccga tttgttagat gccggttcac aattacaggt cgcagcgtta
     3841 gggatgctca accgccaacg cgtctcggcg aacggctttt taagccctta tgcgatggat
     3901 gcgccagcac tgggtatgga cgggccgtac tatacggaag ccacagtcgg tttgtttgcc
     3961 ggactggatc tcgatttgtg gggtgtgcat cgctcagcgg ttgctgccgc cattggcgcg
     4021 cataatgccg cgctggcaga aaccgcagca gtagagctat cgctgaccac gggcgtagcg
     4081 cagctttatt acagtatgca ggccagctat cagatgctcg atctgttaga acaaactcgc
     4141 gatgtgattg attacgcggt gaaagcgcac cagagtaaag tggcgcacgg tctggaagcg
     4201 caagtgcctt tccacggcgc gcgggcacag attctggcgg tcgataaaca aattgctgcc
     4261 gtcaaagggc aaatcactga aacgcgagaa tctctgcgtg cattgattgg cgcgggagcc
     4321 agcgatatgc cggagatcaa accggtggca ttaccacgag tacagaccgg cattccggca
     4381 acgctctctt atgagttgct cgccagacgc ccggatctgc aagccatgcg ctggtatgtt
     4441 caggcgtcat tagatcaggt ggattccgcg cgggcgctgt tctacccgag ctttgatatc
     4501 aaagcgtttt tcggtctgga ctccatccac ctggatacct tattcaaaaa aaccagtcgc
     4561 cagttcaact tcatccctgg tctgaaattg ccgctgtttg acggtggacg gttgaatgcc
     4621 aatctcgaag gcactcgcgc cgccagcaac atgatgattg aacgttacaa ccagtcagta
     4681 ctgaacgcgg tgcgtgacgt tgccgtcaac ggcacacgtc tgcaaacgct caacgacgag
     4741 cgagagatgc aggctgaacg cgtagaagca acgcgcttca cccagcgcgc agccgaggcc
     4801 gcctatcagc gcggcttaac cagccgctta caggccaccg aagcccgatt gccagtgctt
     4861 gccgaagaga tgtcattact gatgctggac agccgccggg tgatccaaag cattcagttg
     4921 atgaaatcgc tgggcggcgg gtatcaggcg gctcccgtcg tcgagaaaaa ataaaatgtc
     4981 taccgcgtga tggctgtcac gcggtatttc gtttcgtcac gtcaaaactg acgacagcct
     5041 gtttttcgtc agagttttga ataaatagtg cccgtaatat cagggaatga ccccaaataa
     5101 aatgtggcat aaaagatgca tgctgtagtc gagagcgcgt atgcgtgatt tgattaactg
     5161 gagcgagacc gatgaaaaaa gtcgtcacgg tttgccccta ttgcgcatca ggttgcaaaa
     5221 tcaacctggt cgtcgataac ggcaaaatcg tccgggcgga ggcagcgcag gggaaaacca
     5281 accagggtac cctgtgtctg aagggttatt atggctggga cttcattaac gatacccaga
     5341 tcctgacccc gcgcctgaaa acccccatga tccgtcgcca gcgtggcggc aaactcgaac
     5401 ctgtttcctg ggatgaggca ctgaattacg ttgccgagcg cctgagcgcc atcaaagaga
     5461 agtacggtcc ggatgccatc cagacgaccg gctcctcgcg cggtacgggt aacgaaacca
     5521 actatgtaat gcaaaaattt gcgcgcgccg ttattggtac caataacgtt gactgctgcg
     5581 ctcgtgtctg acacggccca tcggttgcag gtctgcacca atcggtcggt aatggcgcaa
     5641 tgagcaatgc tattaacgaa attgataata ccgatttagt gttcgttttc gggtataacc
     5701 cggcggattc ccacccaatc gtggcgaatc acgtaattaa cgctaaacgt aacggggcga
     5761 aaattatcgt ctgcgatccg cgtaaaattg aaaccgcgcg cattgctgac atgcacattg
     5821 cattgaaaaa cggctcgaac atcgcgctgt tgaatgcgat gggccatgtc attattgaag
     5881 aaaatctgta cgacaaagcg ttcgtcgctt cccgtacaga aggctttgaa gagtatcgta
     5941 aaatcgttga aggctacacg ccggagtcgg ttgaagatat caccggcgtc agcgccagtg
     6001 agattcgtca ggcggcgcgg atgtatgccc aggcggaaag cgccgccatc ctgtggggca
     6061 tgggggtaac ccagttctat cagggcgtgg aaaccgtgcg ttctctgacc agcctcgcga
     6121 tgctgaccgg taacctcggt aagccacatg cgggtgtaaa cccggttcgt ggtcagaaca
     6181 acgtgcaggg tgcctgcgat atgggcgcgc tgccggatac gtatccgggc tatcagtacg
     6241 tgaaagatcc ggctaaccgc gagaaatttg ccaaagcctg gggcgtagaa agcctgcctg
     6301 ctcataccgg ttatcgtatc agcgagctgc cgcaccgcgt ggcgcatggt gaagtgagag
     6361 cggcgtacat tatgggcgaa gatccgctgc aaactgacgc ggaactgtcg gcagtacgta
     6421 aagcctttga agatctggaa ctggttatcg ttcaggacat ctttatgacc aaaaccgcgt
     6481 cggcggcgga tgtcatttta ccgtcaacgt cgtggggcga gcatgaaggc gtgtttactg
     6541 cggctgaccg tggcttccag cgtttcttca aggcggttga gccgaagtgg gatctgaaaa
     6601 cggactggca aatcatcagt gaaattgcca ctcgtatggg ttatccgatg cactacaaca
     6661 acacccagga gatctgggat gagttgcgcc atctgtgccc ggatttctac ggtgcgactt
     6721 acgagaaaat gggcgaactg ggcttcattc agtggccttg ccgcgatact tcagatgccg
     6781 atcaggggac ttcttatctg tttaaagaga agtttgatac cccgaacggt ctggcgcagt
     6841 tcttcacctg cgactgggta gcgccaatcg acaaactcac cgacgaatac ccgatggtac
     6901 tgtcaacggt gcgtgaagtt ggtcactact cttgccgttc gatgaccggt aactgtgcgg
     6961 cactggcggc gctggctgat gaacctggct acgcacaaat caataccgaa gacgccaaac
     7021 gtctgggtat tgaagatgag gcattggttt gggtgcactc gcgtaaaggc aaaattatca
     7081 cccgtgcgca ggtcagcgat cgtccgaaca aaggggcgat ttacatgacc taccagtggt
     7141 ggattggtgc ctgtaacgag ctggtgaccg aaaacttaag cccgattacg aaaacgccgg
     7201 aatacaaata ctgcgccgtg cgcgtcgagc cgatcgccga tcagcgcgcc gccgagcagt
     7261 acgtgattga cgagtacaac aagttgaaaa ctcgcctgcg cgaagcggca ctggcgtaat
     7321 accgcttaaa ctcacgttaa aagcggcgat attacccaac gcctttgtat caatccggaa
     7381 acgactctct gtttccggat ttttttatac atacctgcgc agtcaaacca gcccatcctg
     7441 gttattagcg acgtcttctc ggttgttctt ctattccagt cacgattgca aatgagaatt
     7501 atctttactc aattctcatc gcggcctaac gtaataatac tttacatttg ataaacaaaa
     7561 tttcggtaag gtgagaaatc atcgaccgtt taactcgttc tattaacggg atattttggc
     7621 gcgattaatg cagcgaaccc ccgtctgata gcatattaat aacctcaaag taatttcagg
     7681 gattgggaag catatgatta aaaaactatt acctttactg gttttaagca caatttccgc
     7741 cacaaccgtt gccgctacac cgccgaatac gttggttgta gcccagggac tggatgatat
     7801 tgtgagtctg gacccggcag aagctaatga actctcaagt attcagactg taccaagtct
     7861 gtatcaacgc gtagtacaac cggaccgtaa tgatccgacg aaaatcactc cgattctggc
     7921 ggaaagttgg caggctgacc ccgcagccaa aacgctaacc ataaaactga aaagcgatgc
     7981 gaaatttgcc tctggtaatc cggtacgccc ggaagatgtg atttactctt atgtccgggc
     8041 tgtcacactg aataaatccg gtgcgtttat tctcaatgtg ctgggatggc agccggaaaa
     8101 tatcgccagc cagttgaaga aaatcgatga ccataccgtc caggtgcaat ggagtgccga
     8161 cgtcagcccg gcgctggcac tgaatattct ctccacacca atcgcctcaa ttgttgacga
     8221 gaagctggtt gcgccaaacg ccaaaaataa cgactttggt aatgaatggc tgaagatgca
     8281 ttccgctggc agcggcgcgt ttaaaatgcg tacttaccaa ccgcatcagg ccatcgtgat
     8341 ggaagccaac accagctcac caaccggcgc cccgaaactg aaaagcgtca tcatcaaaaa
     8401 tgttcccgat ccggcaacgc gtcgcctgct gatccaacaa ggtgatgctg atatggctcg
     8461 tgatctggga gccgatcaaa tcgatgcatt acagggtaaa cctggcgtaa aagtgatgag
     8521 cattgcctcc gccgaacaga actacctggc cttcaatacc ggtaacaagg ataacccgct
     8581 gatgagtaat cctgcgttct gggaggccgc tcgctggttg gtcgattacg acggcatcac
     8641 caaaaatctc ttaaaaggac aatattttac ccatcaaagt ttcctgcccg tgggctttcc
     8701 tggagcactg gaagaaacac cgtttacctt taatccagcc aaagcaaaag agatcctcgc
     8761 taaagccgga atcaaagacc cgcacttcac gcttgatgtc gaaaataaac ctccatttat
     8821 taccatcgcg cagtcaattc aggccagctt tgctcagggc ggtgtaaaag ttgatttgct
     8881 gccagccgca gggagtcagg tctactctcg cgttcgcgcc caccagcatc agggggccat
     8941 tcgtatgtgg ttgcctgatt attttgacgc acattccaac gccagctctt tcgcctataa
     9001 cgatggcaaa tccagcaccg ttgccgggtt aaacggctgg aaaatcccag aactgaacaa
     9061 agaaacgctg gcggccatcg ctgaacctga tagcgctaaa cgtcttgatt tgtataagaa
     9121 aatgcaacaa gaactacaac gtagttcccc gtatgtcttt attgatcaag gcaaaacgca
     9181 aattgtcatg cgtgataatg tgcaggggta tcaacaaggg ttgaatgccg atatggtctg
     9241 gtttgatcag gtaaccaaat aaccctgtat gcaactcgcg tccagccatg ttaccgggcg
     9301 cggtaccagg gctggcctgt attgcaggcc agccgctgat ttatcactgt atggaaagat
     9361 catgtctgtc gttttttcca ctcgcgcgag atggcgaggc aagcgccctc tgctcgcgct
     9421 gttgcaaggg ctgtttaccg ttgctctgac gctactcggc ctgctgctga ttacctttgc
     9481 gctttcggct ctctcaccgg tcgatcgcgt actacaaatc gttggcgatc acgccagcca
     9541 gtccacctat gatcaggtac gccaccagtt aggattggat cagccgttag ccgtccagtt
     9601 ctggcattac ctgatgaatc tggcacacgg cgatctgggc atcgccagct ccaccgtgca
     9661 accagtactc caggacttgc tatcagcatt cccggcgacc attgaactgg caacgttggc
     9721 gcttattgtc ggtgctgttg tcggcgtcat tgcgggtgta ctttgcgccc gttatgtcgg
     9781 ttctccgctg gatttcaccg tgcgaacatt aactctactg ggaaattccg tccctgtttt
     9841 ttggcttggt ctgctgatgc tggcgctgtt ctacgccaga ctgcaatgga gtgccgggcc
     9901 gggcaggctg gatgatattt atcaattcac catagaaccc cgcagcggat ttgcgctgat
     9961 tgatacgtgg ctttctggcg atcgcgaggc atttaccaat gcaatcaacc atttgatact
    10021 tccggtttta ttactggcgt acttctcgct cgccagtatt acgcgcctga ctcgttccgc
    10081 ctgtcttggc gaaatgaata aagaatatat tctgctggcg cgcgccaaag gggccagcga
    10141 gatgacaatt ttgctacgtc atgttttacc caacattcgc ggcaccttgc tcaccgttat
    10201 cgccctcgcc tatacatcaa tgctggaagg cgcggtactg acagaaaccg tcttttcatg
    10261 gccaggtatt gggcgctacc tcactacagc gctcttcgcg ggtgatacca cagcaatcat
    10321 gggaggaacg ctgctgattg gcgtctgctt tgtgttgata aataacttaa ccgacctgct
    10381 ggtccggctg accgatccga ggttacgctg atgccgatgt ttctcttttt acgccgttta
    10441 cgccattcac ccgccgcgtt ttgcgggttg ataattatcc tgttattgat gctaattgcg
    10501 ctcttcgcgc cgtggctggc acctcacgat cctaactggc aggatgcggc ggcgcggtta
    10561 caagcaccga gtcgtcagca ctggctcggc actgacagtt acgggcgaga cctactttca
    10621 cgtttaatct acggcagccg cccggcactg gggctggtgg cattggtgac aatcattaca
    10681 ctcccggtcg gtctgctgat tggcatttta gccgggtact acggcggctg gctggaacgc
    10741 gtgctgatgc gttttactga cgtagtaatg tcaatgccgc gtttaattct ggcttttgct
    10801 tttgttgcca tgctggggcc ggggctggtt aacggcgcac tggcgctggc attaaccacc
    10861 tggcctgctt atgcgcgtca ggcacggagt gagatccaac gtctgcgaca cagtgattat
    10921 cttgccgccg cagaaatgat gggcatccgc ggcaggcgtc tgctggctgg tcatatttta
    10981 ccattgtgcc tgccatctgc cattgtccgt ctggcgctgg atttagcggg tatcattctg
    11041 gcggctgctg gcctgggttt tcttgggctt ggtgctcgcc cgccaatggc ggaatgggga
    11101 gccatgatcg ccgacggtat gcaggtcatt tttgatcaat ggtggattgc cgccgctccc
    11161 ggcgcaatta tcttaattgc cagtctggcc tttaatctgc tgggtgatgg cttgcgcgat
    11221 gttctggagc ctcaacatga ctgactcacg tcttagtatc aacaatctct gcgtcgatta
    11281 tccggagtcc cgggtcgtca acaatgtcag ttttacatta ggcaatgaac gtctggcgct
    11341 ggtcggtgag tcgggttcag gtaagtccat gaccgcccgc gccctgatgg ggctggtgcg
    11401 taaaccgggt gtagtaaccg cggacacact taacattctg gggcgtgatg cactcaccct
    11461 taacgcgcgt ggatggcaac agttgcgcgg taacgatatc gcgatggtat tgcaagatcc
    11521 gcgttacgcg cttaatccag taaagacagt aaaagcgcaa ctggaagagg cgcttacgct
    11581 ccatcaacgt ctgaaccgcc gcgagaaaga agagaaaata aacaccgcaa ttcaggctgt
    11641 cggcctcaat acgcgagtac tgcaaagcta tcctcgtgaa ctttctggcg gcatggggca
    11701 gcgagtgatg attgcaattg cgctcatcaa cgatccccgg gtattgattg ccgatgagcc
    11761 aacatcagca ctggatgtgc gcctgcgtaa tcagattctg gaactgctgg ttacccagtg
    11821 tgaacaacgt cagatggcaa tgttgctgat cagtcacgac ctgccattag ttgcccaatt
    11881 ttgccatcgg gtgctggtga tgtatcaggg aaataaggtg gatgaaatgc acgctgctgc
    11941 gctacctaca gcaacgcatc cttacacgcg cacactgtgg acttgccgcc caaatgcaca
    12001 aacttacggg cagatgctgc cgacgctgga caggacggca atgacgccgg agaaatacca
    12061 tgacgattgt tgaaattaat cacttacagg tgacttttgc tgagaaaacc gcagtttctg
    12121 ccgccagttt ttcggttaat gccggagaga catttagttt aattggcgaa tcaggctgcg
    12181 aaaaatccac gattttgcgc gtacttgctg gattacagcg cgactggcac ggtcaggtgt
    12241 cactgtttgg gcacaccatc aaaccaggaa tgcgttttca gggcgatctc cgccgcaatg
    12301 tgcaaatggt gtttcaggat ccctatgcct cgttgcatcc caatcatact ctgtggcgaa
    12361 cgctggctga accactcaaa attcgtggcg aaagagaaat agaaaaacgc gtccagactg
    12421 cactggaaca ggttggcttg cctttcgacg ccgctcgtcg ctacccacac cagctttccg
    12481 gtggtcaacg gcaacgtgtg gttattgctc gcgccctgtt gttacgacca caacttcttc
    12541 tgctggatga acccacctcg gcgttggata tgtcggtaca agcggaaatc cttaatttgt
    12601 tgaatcaact taagctggaa caccaaatga catatctgct ggtcagtcac gatgccgatg
    12661 tcatcgcgca tatgtctgat cgagcggcat tgatgtccga gggaaaaatt cagcgatttt
    12721 ttgatcgcga cgccatggaa aagggggaac atcggatgga ttaaaaaacc tgacattgca
    12781 ttaattcatt ctgctttcag gtctttttac agttgttttt ttgtccaccc gaacacttta
    12841 tactgtctct cccctaccaa acttcattat caaaatgaaa aaaattatca ctttgatgtt
    12901 atttttgaca ttctttgccc acgcgaacga ctccgagcct ggcagccagt atttaaaggc
    12961 agcagaggcc agagaccgac gcgcacaata ttttcttgcc gacagctggt ttagctccgg
    13021 cgatttgagc aaagccgaat attgggcaca gaaagccgcc gacagcggtg atgccgatgc
    13081 ctgcgcgctg ttggcgcaga tcaaaatcac caatccggtc agtctggact acccacaagc
    13141 aaaagtgctt gccgagaaag cagcgcaagc tggcagtaaa gaaggtgaag taacgctggc
    13201 gcatattctg gtaaatacgc aagcgggtaa accggattat ccaaaggcga tttcgctgtt
    13261 agaaaacgcc tcggaagatc tggagaacga ctctgccgtc gatgcccaaa tgctgcttgg
    13321 tttgatttac gccaacggcg tgggcattaa ggccgacgat gacaaagcaa cctggtattt
    13381 caaacgtagc tccgcgattt cccgaaccgg ttattccgaa tactgggcgg gaatgatgtt
    13441 cttaaacggt gaagagggtt ttatcgagaa gaacaaacaa aaggcgctgc actggttgaa
    13501 cctgagctgt atggaagggt ttgataccgg gtgtgaagag tttgaaaaat taaccaacgg
    13561 ttaaggtagg cgcagcaaac gtcgcatcag gcgttgatgt cggatgcggc gtaaacgcct
    13621 tatccgacct acaaaatcac gctacatcag gctattgcgc tgtctgacgt aggcctgata
    13681 agacgcggca agcgtcgcat caggcgttga tgtcggatgc ggcgtaaacg ccttatccga
    13741 cctacaaaaa ccacgctaca tcaggctatt acgctgtctg gcgtaggcct gataagacgc
    13801 gttaagcgtc gcatcaggcg ttgatgtcgg atgcggcgta aacgccttat ccgacctaca
    13861 aaaaccacgc tacatcaggc tattactcag tcaggcatca acacattgcc gggtggatat
    13921 ccccggcaat cttcagttac tgatccgccg ttttatcaaa tttgcccagc acttcacgct
    13981 cataagccag cgctttctta cggtcaaatt tgtgttccca tttggcaatc accagcaccg
    14041 ccagcgcatt acccaccacg ttcagcgcag tacgcgccat gtcgaggata cggtcaacac
    14101 cggcaataaa cgccagacct tccagcggaa tacctacgct acccagcgtt gccagcagca
    14161 ccacaaacga cacgcccggc acgccagcaa tccctttcga ggtcaccatc agcgtcagca
    14221 ccagaatgat ttcctgccag atggacaggt caatgccata cagctgagcg atgaaaatag
    14281 cggcaatact ttgatacagc gtcgaaccat caaggttaaa agagtaaccg gtcggcacca
    14341 cgaaactggt gatcgacgcc ggtgctccgt aggcttccat cttctcaata attcgcggca
    14401 gcacgctttc agagctggca gtggagtacg ccagaatcag ctcatctttc agaatacgaa
    14461 tcaggatcca gacgcttaac ccgcacaggc gcgccacaat tcccagcact accagcgcga
    14521 agaacagaat ggcgaaatgc accagcagca ccagtttcgc cagtggccac agagacgaga
    14581 aaccaaagtt agccaccgtc accgcaatca gcgcaaacac acccaccggc gcataacgca
    14641 tcaccatgtg agtcacttta aacatggttt cagagatgga gcggaaaacg gtcaccagcg
    14701 gttcacgatg cgtcgcgggc agggatgaaa gccccagacc aaacagcacc gagaagaaga
    14761 tgatcggcag catttcgcct ttcgccatcg acgccacgat gttcgtcggc accagcgaca
    14821 aaatcgtgcc cataatgccg tgggaactgc tttgtaccgc ttccgtggtg ctctgatatt
    14881 tcgagatatc gacggtcgcc agctgcgaca tatccacccc ggcaccgggc tggaagacgt
    14941 tcgccagagt gatccccaaa atgatggcga cggtggtgat cacctcgaag tagataatgg
    15001 ttttcgcacc aatacgcccg agctgtttgg catcaccaac acccgcgata cccaccacca
    15061 gcgtggagat cacaatcggc acaacaatca ttttgatcag atggatgaag atatcacccg
    15121 ccggagagag caaattgacg accagccagt cgcggctgtc gctatggtag tgcaggtagc
    15181 ttcccaggag aatgcccagc accatagcaa acagaatctg ccaggccagg ctgaatttta
    15241 tatttttcat cagaataact tcctcaatgg agcgccgttc cccgttcaga aacgcgagaa
    15301 cgtgacatac tgccagggca tgaattgtgt tgcgttggtt tgtagatttt tttaacgctt
    15361 atgatcagta tcatttgcgc ggtatgttgc gcaacccttt aacaacagag cttttcgctg
    15421 cattaatgca taataacgcg agtttatggt aggattaatg aataaccatt tgttatagaa
    15481 ggatttttat ttgtttaatc tgcaataata ctgattggtg attatttttg attatatgtt
    15541 cattcgatta ttttttatgc agctcaataa cataatcttc atcaccagat gattaatctg
    15601 atctccgctg caacaatttc gccatattaa tcgactcgac cagctgtgtc cggttgatcc
    15661 ggggtgagtt gagatccatc actttttgcc atagttcgat ggcttgcgcg taattcgcct
    15721 gcataaacgc atcggaagcc agcagcatca gagcggtgat ttcgttagag tccagcgcga
    15781 gggctttgtc gatcattgcg cgagcctgga cggtcatatg ttggctggcc tggtaataca
    15841 gcaccgtcgc cagcgccgca tacagttcag cgttctcacc acgtagttgc aacgcctgac
    15901 ggtacgccag cagcgaattg ctgtaatcgt tttgccacag ataatactcg cccagtaacg
    15961 cccactgttc gctgttttgt ggattagcac ggattttatc ctgcaatgcc tgaagctgcg
    16021 cttccggggt ttgctggctg gcaaactgat gcagcggatc gcgctgacgc tgatactccg
    16081 cacgtaccgc ctgccatttt ggacttaaca gataactgcc gacacagaga aataccatca
    16141 aaatcgtgag tgttgtcagc attttcaccg gtatttgggg ctgtttcatc gcttcgccct
    16201 cactcgccag aggatcagtg ccatcagcag taacaacact actgacagcg cccacaacac
    16261 cagcgtctga cccgttaacg gtgggttata gcgaacaaaa tctccgtagc gttcggtcat
    16321 ccagtcaatg atttcgactt cacttttccc ctcttccacc atggtgtaaa cctgatggcg
    16381 catactgact gccaccggcg cattggattc cagtaagttc tgattctggc actgcggaca
    16441 acgtaactgg ctggcaatat ttaacgcctg ttcctgttgt tgcggattgg cgaattgcca
    16501 ggtgtctaca acctgagcgc gcgcaaaaca ggtaaataac agcagtagcg tgagaagccc
    16561 tttattcatc gcgcttcctt ccccgccatc cgcttaacaa tgcgcccgca atcatcaaca
    16621 atcctccccc ccagatccag cgcacaccgc tttgtacata caaacgaaaa gcgtaacgat
    16681 ccgctccggt tttctcgccc atgaccgcat accagtcatg gatgccattc cagcgaattg
    16741 acggttccat catttgctga cgacgtgcct cataaaagcg ccgctccggc gttaattcac
    16801 caatgcgttg ctgatggtca aacagtgcca ctatcgcttt ttcgctggtg taattgcctt
    16861 tggcttgcag atcgaggcgc tcaaaacgga aggtgtatcc tgccagcgtc acctgctgac
    16921 ccggctgtaa attcaggctg atctcctgac ggctgacgct ggaaaccacg atccccgcgg
    16981 caaataacag cacgcccgca tgcgccagca gcgccggaag ctgagcacgt ttgccagaga
    17041 caaacgtcgc cagcacaatc accaccagca tcaacagagc aaacggtaac gtcgcgcggt
    17101 taaaatacgg cgcgccaacg gagaggcgtc cccagcccag caggccgtaa atcatcggat
    17161 aaagcgttcc caccagcacg atcagtagca ctgcgcaaaa cagcaacagc gtagcgagga
    17221 ttaacatttc ccgcgataac cccgaaaaac gcaccgccgg gccaccgtcc cgcgctcgcc
    17281 agccatacag agccagagac gcaaggctaa tcagtgcaaa caggctgaac aacggcacgg
    17341 cgcggacatt atccagcgcg aacgcatgaa ccgaaaccag aatgccagaa cggacaatta
    17401 aggtgcccag cagcgacagc atcagagtaa ctatcgccag taacagcgac cagtggcgga
    17461 aaatcccccg ctggcgtgtc aaagacaaac tgtgcagcag cgcagtggca gaaagccagg
    17521 gtaataaaga ggcgttttcc accggatccc agaaccacca gccgccccag cccagttcgc
    17581 aataggccca ccaggaaccg aggatgatcc ccgccgttaa tgcgctccag ccaggtcgtg
    17641 cccaacgcca gcaaattcgg gcgctgacac catcaaactc gctgcgcagt aaactcgcca
    17701 gcgccacgct cgccgctacc atcaaaccgc cgtagccgag ataaagcagc ggtggatgaa
    17761 agataagacc gggatgttgc agcatcggat tgagatcgcg gccttcgatt gccggtggaa
    17821 atatccgcac gaagggatcg gaccacagca ccacaaacag tagcagtgtg gcgagcatga
    17881 gagataaaac ggctaacgtc agcggaaata gcggatcggt ttgctgtcga taatgccagg
    17941 caaacagcgc gctccaggcg gaaagcagca gcacccaaag cagcagcgaa ccttcatgac
    18001 cgccccacac cgccgccagt ttgagttccc acgacagcag gctatagcta tgttgggcga
    18061 cataaatgac cgagaaatcg ctgatgagaa aacaatacgt cagtacgcca aaggcgagca
    18121 gcaggagcgc gaactgcgcc agaatgccga tgcaagtgag tcgcatcatg gcaggccagc
    18181 gcaaccgcac tcccgcgaag gccgtcaacg gggtcaacac attgacccca agacttaaca
    18241 acagcgccag aaaaccgact tcaggaagga atacgtccag aaaccacctc ttttctggct
    18301 tacgccaccg tcaactgccc ggcgtagaga atgaaaaagc gcagcatcaa cacgccgacc
    18361 aggctggccc cacacgccgc cagcacggca ggaatgccgg aactgcgatt gacccacggt
    18421 ttgagcaaca ttggcacaat cagccccagc ccggcgacac caagccagaa ccaccacgtc
    18481 cagaaaccgc cgcccaatgc cgccaccagc gcacgcactt taccgtcatc gcccagcgcc
    18541 agaccgacaa aaaacgccac cagcaggaag atttcacccc ataccacggg gatttccata
    18601 cggtgtacaa agtgcgcttc cgtggaatgc gggttactgc gttggcgtat cgccatggcg
    18661 atcagcgcca ccgccgcacc ggacgagatg ccggagaaga ggaacagcac cggcaggatc
    18721 gggttattga ggaacggata cgatttcagc gccgacagca gaaagccggt ataagccccc
    18781 agcagcgccg ccagcaccag catcaatgtt tccagtcctc gatgaacggg cgtcagcagg
    18841 ctcagaacct ttcgcacgat ccccagcttc ggcaaccagc gttgttgcag ggcaagcaaa
    18901 tcatgttcaa agattttcgc cagccacagc accagcacca ccatgtagag ctgaaacagc
    18961 atcaccccca tcgacatcac cgaggtaaaa ctgtagtgga acatcagctt ccagaaggtc
    19021 cacggtcttg tcaggtggaa gacgagaatc aacagaccga ggatcaccgc gcccggcccg
    19081 acaatcagcg tggtgcgcag caacgtactg tctgcaccgc ctgcctgagg gtagaagcga
    19141 cgtaacagta cggccagcgt caccagaccg gcagaaatac caatcaaaaa caggtagatg
    19201 gcaatcggcc agtcccacac cagcgattca aaatgaaatg cggaagtctg cgtcattggc
    19261 tcacctcccc gtatttaaac ggtacgcggt acagcttcgg tttggttccc agcgccagct
    19321 tgtagcggta agtgggcttc tggcgcagca gttgcgaaat ctcactgctg ggatcgtcca
    19381 gattgccaaa cgtcagcgcc ttcgtcgggc aagcctcaac gcacgcgggc agcttaccgg
    19441 cctgcaaatg ggtcttacgg cagaaatcgc atttgtccgc cgtcttcgtg accggatgga
    19501 taaagcgcac gcggtacgga caggcggcga tgcagtactg acaaccgacg cagagatccg
    19561 gattaacatc gacaatccca ctggcagcgt cgcgaaaaga cgctccggtc gggcagacgt
    19621 caacgcacgg cgcatgatcg cagtgctggc aagacttacg gaagaagcga tatttcacat
    19681 caggaaattc gccctgcggc tcgctacgaa taatcgtcag gcgcgagacg ccttccggca
    19741 ccttgttcac tttccgacaa gcatccatac aggcggtaca gccgatgcat aacgactcgt
    19801 catgcaccat gccgtaacgc accccattga tattcaacgt tttcgccacc acgcgccctg
    19861 cggtgccgct gacggcggcc agcacaccga cgccggtgag aaactggcga cgtgaccagg
    19921 tcatggctgc tccttaagca acggaacaga cgccgggtta aagttcggat tggtgcgttg
    19981 atcgctgtgg caatcgacgc aaatcttaat ccgtcctttg tcacttaacg tctgcatcgt
    20041 atcttgctgt ggatgcaggg aatggcagct ggcgcacgcc actttggtta cgtggacatc
    20101 gtgcggccag aacgcttttt gcaactgttc cggcagatgg caggacatac aaacactgtt
    20161 ctgctctcca accttgtaca tcggctcgtt aaagcgcatc acatctttca ccccttcgcg
    20221 gtgttgcggt gatggctgac cgtggcagtt ggtgcaggtg accggcagtt tgttattcgg
    20281 gttgataaca gaagcatgtt tgccatgcat accttcggta tctggcttat gacagtccag
    20341 acaggcggca tccgggttac gctgctgggt aacttcgtag cgatcatcag atgcctgcgc
    20401 cgcaggggta gcggtaattc cgttcaggct ccacaacagg cccgacgcca gcaccccggc
    20461 agttaataac gaacgtaata cgctcatatt cactccattg tgttttccgc cccttgcgag
    20521 gcggaacggg gttattggct taacagaccg tttttacgtg cctgctcttc ccactgcggg
    20581 atcaccgttt tgatgaagtc ctgcttctcg gccttgattt gctccatgtt caggccaatg
    20641 gcctgctggg ctttctcttt ggtcgagata tccgggatct ggatttcatg ggtaatgcct
    20701 ttggtcgcca gcaggcgcgc cagtttggtg cgtgcatccg ccgctttatc catcgccgta
    20761 ccgagcatcc tcagaccttc ttccggtgcg tgcatatgaa tgccgtggga agcgatcgcc
    20821 aaatcccaac gccactgggc atgacggata tcgtcctgaa tcggcttcat ttcggcttcc
    20881 gttgcgcctg catccagcgc cgctttcgct tcgaagtgag cgtgaaccag ttgatcttca
    20941 accttgattt tcaggtcgtt aatcgactgc ttacgttccg cgaccacttt ttgcaaggca
    21001 gctttgtcct gggtatggca gttcgcacaa gtctgggcga agttatcaaa cggattacca
    21061 attttatggt cggtgtagag tttgccttcg gcgttctgca cttttggcat atggcaatcg
    21121 atacaggtca cgttgttttt gccgtgaatg cccgctgtcc aggtttcata ttccgggtgc
    21181 tgcgctttca gcattggcgt tttcgacagg gagttagtcc agtcagagaa ggcaattttg
    21241 tcgtaatact gctccatatt ttcgactttc atgccgtcat cccacgggaa tttaaccgct
    21301 ttgtttttgc cgtcgaagta atactccaca tggcactgac cgcaaaccat cgattgctgg
    21361 tcgaaacgtc cggctttctc gaaaggttta ccaatggctt ccatcgcgcg agccgcatac
    21421 ggacgggaaa gggttaactc cggtttgcct ttggcgaact ctggtgaggc ggtgttatgg
    21481 caatcggcac aacctaagtt gttgacgatt tccgggccgc cgcgcgccca ttttccgtgg
    21541 aagtagccat cttcgccgtc tttctggatc agacgcgcca catccgggct tttacaactc
    21601 cagcacgcca tcggtagcgg accatcttca gcgtttttcg gcgcaccggt acgcagggtt
    21661 tcacgcacat cggtcacagc aaaggcatgt ccacgcggct tgttgtaatc gcgcgagaag
    21721 ggataccctg cccacaggat caccagccgt ggatcttccg ccagggcgtc aacacgctct
    21781 gactgttccg aggtagcttt ccaggagaga tattgatcgg gatgctgcgg ggcaaaggtt
    21841 tcattcttcg cttccacagt tacaggtgtt gcgggagcag ccgtttgttc agcgtgaaca
    21901 gaagtgaaaa agaaaaaagg aatcaataag ctgaagatac ggcgtgcgtt tatttttatc
    21961 cttgtcatag ggcttcatcc gaattgcgcc attgttgcaa tggcggtttt tattgttttt
    22021 cacgacagta accgcaccta cactgtcatg acattgctcg tccctatgtg taacaaataa
    22081 ccacactatg aatgttgtct ttaatcaatt gtaagtgcat gtaaaatacc actttagagt
    22141 tagtcagtat cttcctcttt ttcaacagca tgcataactg catgttcctc aaagaattaa
    22201 tcaacttttg ttgctgacct tcaaaaatta ccctgccgtt tatttgcaca attctacttt
    22261 tgcgtgatct gtcgcccaaa tactaaacaa aactgccaat acccctacat ttaacgctta
    22321 tgccacatat tattaacatc ttacaaggag aacaaaagca tgagtcaaat tcacaaacac
    22381 accattcctg ccaacatcgc agaccgttgc ctgataaacc ctcagcagta cgaggcgatg
    22441 tatcaacaat ctattaacgc accagatacc ttctggggcg aacagggaaa aattctcgac
    22501 tggatcacac cgtaccagaa ggtgaaaaac acctcctttg cccccggtaa tgtgtccatt
    22561 caatggtacg aggacggcac gctgaatctg gcggcaaact gccttgaccg ccatctgcaa
    22621 gaaaacggcg atcgtaccgc catcatctgg gaaggcgacg acgccagcca gagcaaacat
    22681 attagctata aagagctgca ccgcgacgtc tgccgcttcg ccaataccct gctcgaactg
    22741 ggcattaaaa aaggtgacgt agttgcgatt tatatgccga tggtaccgga agccgcggtc
    22801 gcgatgctgg cctgtgcccg cattggcgcg gtacattcgg tgattttcgg cggcttctcg
    22861 ccggaagccg ttgccggacg cattatcgac tccagttcac gactggtgat cacttccgac
    22921 gaaggtgtgc gtgccgggcg tagtattccg ctgaagaaaa acgttgatga cgcgctgaaa
    22981 aacccgaacg tcaccagcgt agagcatgtg gtggtactga agcgtactgg tggaaaaatt
    23041 gactggcagg aagggcgcga cctgtggtgg cacaacctgg ttgagcaagc cagcgctcag
    23101 caccaggcgg aagagatgaa cgccgaagat ccgctgttta ttctctatac ctccggttct
    23161 accggtaagc caaaaggcgt gctgcacact accggcggtt atctggtgta cgcggcgctg
    23221 acctttaaat atgtctttga ttatcatccg ggcgatatct actggtgcac cgccgatgtg
    23281 ggctgggtta ccggacacag ttatttgctg tatggcccgc tggcctgcgg cgcgaccacg
    23341 ctgatgtttg aaggcgtacc gaactggccg acgcctgccc gtatggcgca ggtggtggac
    23401 aagcatcagg tcaatattct ctataccgcg cccacggcga ttcgcgcgct gatggcggaa
    23461 ggcgataaag cgatcgaagg caccgaccgt tcgtcgctgc gcattctcgg ttccgtgggc
    23521 gagccaatta acccggaagc gtgggagtgg tactggaaga agatcggcaa cgagaaatgc
    23581 ccagtggtcg acacctggtg gcagaccgaa actggcggtt tcatgatcac gccactgcct
    23641 ggcgctaccg agctgaaagc cggttcggca acacgtccgt tcttcggcgt gcaaccggcg
    23701 ctggtcgata acgaaggtaa cccgctggag ggggccaccg aaggcagtct ggtgatcacc
    23761 gactcctggc cgggtcaggc gcgtacgctg tttggcgatc acgaacgttt tgagcagacc
    23821 tacttctcca ccttcaaaaa tatgtatttc agcggcgacg gcgcgcgtcg cgatgaagat
    23881 ggctattact ggatcaccgg gcgcgtggac gacgtgctga acgtctccgg tcaccgcctg
    23941 gggacggcag agattgagtc ggcgctggtg gcgcatccga aaattgccga agccgctgtg
    24001 gtcggtattc cgcacaatat taaaggtcag gcgatctacg cctacgtcac gcttaatcac
    24061 ggggaggaac cgtcaccaga actgtacgca gaagtccgca actgggtgcg taaagagatt
    24121 ggcccgctgg cgacgccaga cgtgctgcac tggaccgact ccctgcctaa aacccgctcc
    24181 ggcaaaatta tgcgccgtat tctgcgcaaa attgcggcgg gcgataccag caacctgggc
    24241 gatacctcga cgcttgccga tcctggcgta gtcgagaagc tgcttgaaga gaagcaggcc
    24301 atcgcgatgc catcgtaacc cacaattgcc ggatgcgctc cgggcaacaa gtcggaaacg
    24361 atttgtaggc ctgataagac gcgacagcgt cgcatcaggc attgattgcc ggatgcggcg
    24421 tgaatgcctt atccggccta cattcggcaa gggttaccca agcgttaacc ttctcccata
    24481 agggagcggg aattaaaaca atccctacat tacctctgga gaatctgtga tgaatggtac
    24541 gatttatcag cggatagaag acaatgcgca tttcagggag ttagtcgaaa aacggcaacg
    24601 gtttgccacc atcctgtcga ttattatgct ggcagtttat atcggcttta ttttactgat
    24661 cgccttcgcg cccggctggc tgggcacccc gctgaatccg aacaccagcg tcacacgcgg
    24721 tattccagtc ggtgtcgggg taattgtgat ctcctttgtt ctcactggta tctacatctg
    24781 gcgggcgaac ggcgaattcg accgtcttaa taacgaagtc ctgcatgagg tacaagcatc
    24841 atgaaaagag ttctgacggc gcttgccgcc acactccctt tcgcagctaa cgccgcggat
    24901 gctattagcg gggccgtaga gcgccagcca acgaactggc aggcgattat tatgttcctg
    24961 attttcgtcg tgtttacgct cggcattacc tactgggcgt caaaacgcgt acgttcgcgt
    25021 aatgactact acaccgcagg cggcaatatc accggttttc agaacgggct ggcgattgcc
    25081 ggggactata tgtccgccgc ctcattcttg gggatctccg cactggtgtt tacctccggc
    25141 tatgacggct taatttactc gctaggcttc ctggtaggct ggccgatcat tttgttcctg
    25201 attgccgaac gtctgcgtaa cctcggtcgc tacacctttg ccgatgtggc ctcttaccgt
    25261 ctgaaacagg ggccgattcg tattctttcg gcctgtggtt ctctggtggt ggtggcgctt
    25321 taccttatcg cccagatggt gggcgcaggt aaactgatcg agctgctgtt tggccttaac
    25381 tatcacattg cggtagtgct ggtcggcgtg ctgatgatga tgtacgtcct gttcggcggc
    25441 atgctggcga ccacctgggt acagattatc aaagccgtgc tgttgctgtt cggtgccagc
    25501 tttatggcct ttatggtgat gaaacacgtc ggctttagct tcaacaatct gttcagcgaa
    25561 gcgatggcgg tacacccgaa aggtgtcgac atcatgaagc cgggcgggct ggtgaaagat
    25621 cctatctccg cgctctcttt ggggctagga ctgatgtttg gtacggcggg cttgccgcat
    25681 attctgatgc gcttctttac ggtcagcgat gcccgcgaag cacgtaagag cgtgttctac
    25741 gccaccggat ttatgggcta cttctacatc ctgaccttta ttatcggctt tggcgcgatc
    25801 atgctggtcg gtgcgaatcc ggaatataaa gacgcggcgg gccatctgat tggtggtaac
    25861 aacatggcag cagttcacct ggccaatgcg gtgggcggca acctgttcct cggctttatt
    25921 tcagcggttg ctttcgccac cattcttgcg gtggttgcgg gtctgacgct ggcgggcgca
    25981 tcggcggtgt ctcacgacct gtacgctaac gtcttcaaaa aaggtgcgac cgaacgtgaa
    26041 gagctgcggg tatcgaaaat caccgtactg atcctcggtg tgattgccat tatcctcggc
    26101 gtgctgtttg agaatcagaa catcgccttt atggtggggc tggcgtttgc catcgcggcg
    26161 agctgtaact tcccgatcat tctgctttcc atgtactggt cgaaactgac tacccgtggc
    26221 gcaatgctgg gcggctggtt aggactgatt actgcggtgg tgctgatgat cctcggcccg
    26281 acgatttggg tacagatcct cggtcacgaa aaagccatct tcccgtatga atacccggcg
    26341 ctgttctcta tcagcgtggc attcctcggt atctggttct tctcggcaac ggataattca
    26401 gcggaaggtg cgcgcgagcg tgaactgttc cgtgcgcagt ttatccgctc ccagactggc
    26461 tttggcgttg agcagggccg cgcgcattaa tctctcccct tccccggtcg cttgatcggg
    26521 gaatttcttc agaacatcat cctggccacc agcggcgaaa ccaataccat caatacacct
    26581 gaaagcatca tgaccaggct tgcaactacg ccttcttgtt gacccagttc atacgaccgc
    26641 gcagtcccgg caccatgaga tgccgcgccg aatccagcac ctttcgccat cccttcgcga
    26701 atagagagac gtaaaaacaa cacgtcgcca atcgccatgc caaacacacc ggtgaccacc
    26761 acaaacagcg ccaccagatc aggctgtccg ccgagcgact ggctcgctgc caaagcaaat
    26821 ggcgtagtga ccgaacgcac cgccaggcta cgctggatct catctggcag ggtaaacaat
    26881 cgcgccagcc acactgagct ggtcactgcc accaccgttg ccgtcagtac gcctgccgtc
    26941 aacgacatcc agtgacgctt aatgatcgcc cgattgtcat acactggtac cgcaaaggcg
    27001 atggtggccg gacctaatag ccatagtaac cagtgcgctt cgccaatata gttttgccag
    27061 gaaatattgc caaaaaccag cagcataacc agtagcgtag gcgtcagcac cagaggcatc
    27121 aatggcaatt tacgaaagcg gcgataaagg cgtttgttaa gaaaatagat aactagcgta
    27181 attgccagac agagcatact tagctgaaaa ttagccacgg tttaacctgc gcatttcata
    27241 gcgatagact ttttccacta cccaggcagt cgtgcccagc accatcaccg tactcagcgc
    27301 gatcacagca aaaatacgcc agccatccac cagtaatagt tgtgcatagt tcaccacagc
    27361 caccacggca gggacaaaaa acagcaacat ttcggccaat agccaacgcg ccccggctcg
    27421 aacccattgc agaggaagaa tgcgacaaac aatcagcgcc agcatcagga tcatccccac
    27481 caggttggca ggcagcggca aatgcagcca ggagacgaga tactgcgcaa aaataaataa
    27541 acctgcatac agcacaacct gaaaaaatac ctggatagat tgcactacgg caggggtaat
    27601 gcggcgaagc gccacgggca tgagaggata tcctgaaaat aaaacagaat ggtaagtata
    27661 gagagcgctc gcccctgact gaaatgaatt aaaatcattg caggtatagt caaagggaat
    27721 agttatggat atcagaacgc tgcgttattt tgtcgaagtc gtgcgccagc aaagcttcac
    27781 ccgcgcagcg gagaagttgt ttgtcaccca accaactatc agcaagatgc tgaagaatct
    27841 cgaagatgag ctgaactgta tattgttgat ccgcgatggt cgtcggctgc ttctcactga
    27901 tactggtcgc gtcgtatttg agcgcgggct ggctttactg gctgagtttc ggcaactgga
    27961 agctgagctt agcgatatca atcagcttaa aaccggcgtt ttgcggctgg gaatcccgcc
    28021 gatggttggg atgttgatgg ccgggccgat tggccttttt cgccagcgtt accccggcgt
    28081 agaattgaaa atttcggagt ttggtggtct gacggtacaa caagcggtga tgaacggtga
    28141 actggatatg gcactgacag ccctgccagt agaagaaagc ggcctgacaa cgttatcgct
    28201 gtttagtcat ccactatgtg tactgacgcc acattcaggt aaatggcttg aatgtgatgc
    28261 cgtctcaccg gagacattgg cggaacatcc tctacttatc tataacgaag attttgcctt
    28321 aagtaagcaa ttgatgaagt tattcgatga acatggcgtt aagccgagaa ttgcagttcg
    28381 cagcgggcaa tgggattttc tcgccgcgat ggttcaggcc ggtgtcggaa tagccatatt
    28441 gccggaaccc atttgccagc gattagataa aaacacctta aaatggctac cgctggaaag
    28501 cgaactaagc tggaaattag gaatgatttg gcgtgagggc gtttaccttt cccactctgc
    28561 ccacgcgtgg ctgagctgtt gtgaaggata ctggttaacg ccagagtaag tcgggttggc
    28621 gggctcgccc gccaaccatg cagattactg attttcctca atcagcaacg cttcaagcaa
    28681 atcgagatcg tgcagtaatt tttgcagcgt ttcgttactg atctcccgcg tggcgcgcag
    28741 gtggtacagt tcagcacgtt cagaacgcaa tgccgccaga cggaagcgac gctccaggtt
    28801 ctcttccagg attgaacttt ctacatcatt acgtccatcg gcgcgacgac gcaggttacc
    28861 aatgacgcga gaactaacct ctgtgagcag ctggttatcg atattctctt cggtatccgc
    28921 cgccagacgc tcctccattt tctggatcgc cacaatcgcc acttctgccg ttgccgctcg
    28981 tgcaatacgt tcctctttca attgttgcga atggtccgcg acttctatgt gttgtagcag
    29041 aataggcaac atcaccacgc cgacaaacaa tgagaagaga atgacgccag ccgccaggaa
    29101 caccaactca tagcgcgccg ggaagacgtt accatccggc aagagaagcg ggatagagag
    29161 cacaccggcc agagtgatcg ccccacgcac cccggcgaaa gacgcgatca ggatttctcg
    29221 tgtagtccac gaaccaaact ccatcggctt cttcttcagg aagcggttgc tgaacttttt
    29281 catcgtccac agccagccga aacggaccag catcagcgcc gcatatatca gaataatatc
    29341 ggtaaacagc atccagattt cgacgttagg gtcgatttct gccgccatca gcgacgtctc
    29401 cagaatcccc ggcaactgca gacctaacag caggaacacc atgccgttaa agacaaattc
    29461 cagcatcgcc caggtgctgt ttgcgcgcag gcgcattgcc agcggcgcac ggcgcatcac
    29521 accggagcgg gtgatggtca tcccggcggc aaccgcagcg aggatgccgg aaacgccaat
    29581 atgttccgca atcagataag aagcgaatgg cagcaagaac agcagaacga tctgcgtcgc
    29641 gggttcatca ccgccccagc ggctgaggaa tcgcagcgag cgaccataca gccagctcac
    29701 cacaaaaccg gcgagaatac cgcctattgc cactttcata aattcgaccg tcgcaccgcc
    29761 gacggtgaag atcattgtcc ccatcgccac tgccacggca aacttcaacg acaccaggcc
    29821 ggaggcgtca ttcatcaacg cttcgccctg caaaatgccc atgatttttt tcgggatgcg
    29881 cccctcgcca acaatcccgg agagcgctac agcatcggtc ggagaaagca ccgcagccag
    29941 cgcaaaggcg gggatcagcg gaatgcccgg caccacccag taaataagga agccaatgcc
    30001 gaccacggtg accaccacca gcgccagcgc gaggccgaaa atctctcgcc catgttcgag
    30061 aaattcacgg gtcggcgttt tccagccatc agcgaacagc aacggcggga taaacaagac
    30121 taaaaagagt tcaggatcaa actccacatg caaaccaaac gtcggccacg ccagtagcgc
    30181 accgatggcg atttgcataa gcggaagcgg gatctgaaag ggcatgacac gagtgaccac
    30241 cccggacagc gagaccacga gggtcattat cagtatggtg aagaagattt ccatgcgttc
    30301 cctgtttgcg atgtttttta tgatctgaga aaggcgttat gcctgaattc tcattttttc
    30361 agaatatcgc aaaagctcac acaatgtgcg gagaaaataa aaacgggcag ccattgccac
    30421 ccgttttcag aatcctatgt aagattagat agcccagcca cccgcataga aggtcaccag
    30481 cgccacggca ataacgaccg taccgatgtt caacttgcgc cattcgccgg aaaccagacg
    30541 accaatcacc agagtcgcga agccgatcat gatgcctgtt acgatgttac aggtcagcac
    30601 gatgaatacc gccgtaacca gacccgccat cgcatcaaca aaatcagcaa agtcgatttt
    30661 cgccacgttg ctcagcatca gcaggccaac gtacatcagc gccggagccg ttgcgtaccc
    30721 cggaacgagg taagagagcg gagagaggaa cagaatcagc aggaacagca cgccaacggt
    30781 gatagccgtc aaaccggttt taccgcccgc cgccgtaccc gccgcagact cgatgtatac
    30841 cgctgccgga gctgcaccca ccaggccaga aaaaacgctg ctcatggagt cggtggtcag
    30901 tgctttcccg ccgtcgatga tctgcccatc tttatccagc aggttcgcct gaccggcgac
    30961 ggcacggata gttccggtgg catcaaatac tgctgtcatc accagtgcca gaacacttgg
    31021 caggactaca ggattcagcg cgcccataat gtccaggctg ccaatcagtg aattgccgtt
    31081 ttcatcgctc aatgaaggca tggcaaaaac gccggagaaa tggacgttag gatcgaagat
    31141 caaaccgaca attgagatac cgataatggt cagcagaatg ccaccaggga ctttcagttt
    31201 ttccaggccg ataatcactg ccagacccac caacgacatc atcaccggaa aggtcgtgaa
    31261 atcccccagc gcgacgggca gaccatcaag tgggttttta atcaccagac cgacaccatt
    31321 agcagcaatc agcagcagga acagaccgat accaatcccc gtgccgtgcg ccacaccgtg
    31381 cggcaagttg cgcaaaatcc agctacggat acccgtggca gaaattaccg taaacagcac
    31441 acccatcagg aacacggcac ccagcgcgac aggtacgcta atatgttgcc ccagcaccaa
    31501 actgaatgcg gtaaacgccg tcagagagat ggcgcaacca atcgccaacg gcagattagc
    31561 ccacagaccc atcacgatag aaccgagtcc ggcaaccaga caggttgcaa cgaaaactgc
    31621 cgcaggcggg aagcccgctt tacccaacat gcctggaacg acgataactg agtagaccat
    31681 cgccagaaac gttgttaacc cggcaaccac ttcctgacgg acagtgcttc cacgttgtga
    31741 aattttaaac caggcgtcga gtgaaccgcc ggtacgcgct gatggcgtag acataagaaa
    31801 acatcccctg agagtttaat tttcgtcaag atgcgtggcg gacaatcccc tgccagcgaa
    31861 acgttatctt cctgctccag gtttgcgaca atttgctgcg tcgcaaaaaa agcaaacgtt
    31921 tagctccgcg catacaaaac gggtggtaaa aaaagcaaac gattatccgg cgatcatagc
    31981 ggctttttca accgaagttt gctgcttttt tctcttttat tgtcttgacg atgaaaaacc
    32041 aggcaattga tgcgcaaacg ttatcgtcga tgcgcaagat tgcagcgtta tcgagccgta
    32101 taccgttcag gattgcgata aaaaatcgtc tgagatgaca aaaatattgc ggctaaaaag
    32161 tgtactttta cactggcgaa gaaacgccgg tcacatacaa ttaaagcatc aacaccaacc
    32221 ggaacctcca ccacgtgctc gaatgaggtg tgttgacgtc gggggaaacc ctcctgtgta
    32281 ccagcgggat agagagaaag acaaagaccg gaaaacaaac taaagcgccc ttgtggcgct
    32341 ttagttttgt tcatcttcca gtaagcgtgc gccggtacct tcttctccta atcggtcgcc
    32401 cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac atccatccag
    32461 ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc gccattggga
    32521 cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca cgccaaacgc
    32581 ttcacgaatg gttgcgagcg gaatgccaat acgctgagca attttaataa ttgcgacata
    32641 tcgcaacaca tcacgtttat atcgccgctg attgccgcta ttacggatac tggtaatcaa
    32701 ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt tcgccacttc
    32761 gccgggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc gctttacctc
    32821 aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa cactgaaaag
    32881 aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga ttgacgagca
    32941 tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tctggctatt caaagtggta
    33001 cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca ttcgccagcg
    33061 ccgtctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt ttgatatcgc
    33121 aatggacctg ggttatgtct ctcagcagac cttctcccgc gttttccgtc ggcagtttga
    33181 tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg ttaactcccg
    33241 cgcgggtaat taccccatcc attgcataaa cacctgcggc ggcatcgcct tcgcaaaaaa
    33301 ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgcg cctcagtttc
    33361 gacaccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga tgtgttccgc
    33421 aatcaaatga ctggttttgt gggtggtcag cgtctcaaca aacgatttat cgattttcaa
    33481 aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac caaaatcatc
    33541 aattgccact tcgtaaccgg ccttgcggaa agccagaata atcggcgtca ttttgtcgac
    33601 atcaagaaat gcatgttcag tcacttcaaa tttaatttgc tgcgggcgca ccgcatgttg
    33661 ctctgttttc tgattggttc gcgctatcaa ccgtgaggta tgaaaatcgg aggctgacag
    33721 gttaatagaa acatagcgat cggtatgtgt tgccagatac gcgcccaaat cacggaagac
    33781 attatcaata acataatcag ttacctgttc gatcatcccc tccttttctg ccagcggaat
    33841 aaactctgcc ggattcatta cttgcccctg ctcaccaggc caacgtaaca acgcttcagc
    33901 gccgatacat ttttctgttt tgatatcgat tattggctgg taataaaggc aaagctgatg
    33961 tttttcgagg gcgcgttgca atttgcgttt gggagacaaa tagttttgtc gaatacgtag
    34021 ccagagtaat agcagaacaa gactccccaa aataccggcg ggtaacgcaa aaataagatg
    34081 attataaaaa tgggttataa gacgttgata tgaagtcgca acaatggctg caattgggcg
    34141 ttttgtcgaa taaactgtcg catataaata gccatttcgt tgtacggtta aatcattcaa
    34201 atgaatcagc ggagaaaacg ttgctgccga ggcctcgttg cttaacgaga aaaaggtttt
    34261 cgtcaccgta tcatacacac cccattgcaa tgtcggatca tcagacatca cttcactcca
    34321 gaagagaggg ttgataaccg ccacataatt tccccgctgc atgtaggtca ttttatagcc
    34381 agagaaaaaa ggcgtatcac ggtaataata gatagaaacg ttaggttcac gcttataatc
    34441 ggccggtgca atcgtatagc catttacaga cgctatcagc gatgagcata aaaaatggtt
    34501 atcacgagca taaatcaatt cattaatata aagatagcca cgaataatat tcaacattcg
    34561 cttttgatgg gctggagtgc aaacttgccc ctgatagcgt tcagctgcat cgctcactaa
    34621 atctgcctga cgaatgacca gctcagattt atccaacgcc agctgagcaa aagttcggag
    34681 ctgattattc acttctgatt ttgcccaaaa gaaggcaatc catagcgaca gaatgatggg
    34741 aaagagaact aaaaagataa tgcccggaaa cgccagtaat tggtgtcgtg cacgatgact
    34801 cattgctatc aattccctgc ctgtttatac ccggttattg caaggtgaaa ccctggctac
    34861 cgacgtcttt agataaatct tacggtataa atagcacttt tgcctgatat aaaattgaga
    34921 cctttttaat cgtcaccagg atgacgataa ttcattgatc ttaaaaatta atcaccttca
    34981 ggaatgcaga ggcggcggga agatataccc ttaatgccgc tgaaaaaaca cataatctgt
    35041 acggcaataa aagtcacggc ctaatctggc taacaaacaa ccaattgtga tatagttcac
    35101 aaaattcatg aaacaaacag agtgtttcat ttttgtgttc tgcaagcccc cgcgatagcc
    35161 agagtgtatc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcag ctcttttccg
    35221 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    35281 agtgccgtaa tgatgtttct ggctagcacg ctcaacatcc gttttcgtcg ggctgattat
    35341 gtcgggcttg cagtgatcag cagcggtctg ggcgtagttt ccgcctgctg gttcgcaatg
    35401 gggttgcttg gcatcacaat ggcggatatc accgccatct ggcacaacat cgagtcggtg
    35461 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ttgatatgta
    35521 gaagcctcca taacggaggc ttctttttta tggctggcag atgctttaat catccacctt
    35581 aaaacaatat aacctattgt tttaaagaca aatcagaacg gaatatcgtc atcaaagtcc
    35641 atcggcggct cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg
    35701 ccgctgaact gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga
    35761 ccaccaccga tattgccacc tgcaggagcg ccaccaccct gacgaccacc caacatctgc
    35821 atggtgccgc caacgttcac cacgacttct gtggtatagc gatcctgacc ggattgatcg
    35881 gtccatttgc gggtacgcag ctggccttcg atataaacct gagaaccttt acgcagatat
    35941 tcgctggcca cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgctct
    36001 ttcatctcgc cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca
    36061 actgcgccac catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga
    36121 ataaccttgt ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa atcgttctga
    36181 ataagtgtaa acgcgcgatt gtaccattac caatagcgct tttactatgt tgtgacctcg
    36241 gttccgggaa acaaacctgg ctagacattg ttacacaaca ctccgggtaa tgcattccaa
    36301 tactgtatat tcattcaggt caatttgtgt cataattaac cgtttgtgat cgccggtagc
    36361 accatgccac cgggcaaaaa agcgtttaat ccgggaaagg tgaatggata agatcgaagt
    36421 tcggggcgcc cgcacccata atctcaaaaa catcaacctc gttatccccc gcgacaagct
    36481 cattgtcgtg accgggcttt cgggttctgg caaatcctcg ctcgctttcg acaccttata
    36541 tgccgaaggg cagcgccgtt acgttgaatc cctttccgcc tacgcgcggc agtttctgtc
    36601 actgatggag aagccggacg tcgaccatat tgaggggctt tctcctgcca tctcaattga
    36661 gcagaaatcg acgtctcata acccgcgctc tacggtgggg acaatcaccg aaatccacga
    36721 ctatttgcgt ctgctgtacg ctcgcgttgg tgagccgcgc tgcccggacc acgatgttcc
    36781 gctggcggcg caaaccgtca gccagatggt ggataacgtg ctttcgcagc cggaaggcaa
    36841 gcgtctgatg ctgctcgcgc caatcattaa agagcgcaaa ggcgaacaca ccaaaacgct
    36901 ggagaacctg gcaagccagg gttacatccg tgctcgtatt gatggcgaag tctgcgatct
    36961 ttccgatccg ccgaaactgg aactgcaaaa gaaacatacc attgaagtgg tggttgatcg
    37021 cttcaaagtg cgtgacgatc ttacccaacg tcttgccgag tcgtttgaaa ccgcgctgga
    37081 gctttccggt ggtaccgcgg tagttgcgga tatggacgat ccaaaagcgg aagagctgct
    37141 attttccgcc aacttcgcct gcccaatttg cggctacagt atgcgtgaac tggaaccacg
    37201 cctgttttcg tttaacaacc cggcgggtgc ctgcccgacc tgtgacggcc ttggcgtaca
    37261 gcaatatttc gatcctgacc gcgtgatcca gaatccggaa ttgtcgctgg ctggcggtgc
    37321 gatccgtggc tgggatcgcc gcaacttcta ttatttccag atgctgaaat cgctggcaga
    37381 tcactataag ttcgacgtcg aagcgccgtg gggtagcctg agcgcgaacg tgcataaagt
    37441 ggtgttgtac ggttctggca aagaaaacat tgaattcaaa tacatgaacg atcgtggcga
    37501 tacctccatt cgtcgtcatc cgttcgaagg cgtgctgcac aatatggagc gccgttataa
    37561 agagacggaa tccagcgcgg tacgtgaaga attagccaag tttatcagca atcgtccgtg
    37621 cgccagctgc gaaggaaccc gtctgcgtcg ggaagcacgc cacgtgtatg tcgagaatac
    37681 gccgctgccc gccatctccg acatgagcat cggtcatgcg atggaattct tcaacaatct
    37741 caaactcgca ggtcagcggg cgaagattgc agaaaaaatc cttaaagaga tcggcgatcg
    37801 tctgaaattc ctcgttaacg tcggcctgaa ttacctgaca ctttcccgct cagcagagac
    37861 actttccggc ggtgaagccc agcgtattcg tctggcgagc cagattggtg caggcctggt
    37921 tggcgtgatg tacgtgctgg atgagccgtc tatcggcctg caccagcgcg ataacgagcg
    37981 cctgctgggt acgcttatcc atctgcgcga tctcggtaat accgtgattg tggtggagca
    38041 cgacgaagac gcgattcgcg ccgctgatca tgtgatcgat atcggtccgg gtgcgggtgt
    38101 acacggcggt gaagtcgtcg cggaaggtcc gctggaagca ataatggcgg tgccagagtc
    38161 gttgaccggg cagtacatga gcggtaaacg caagattgaa gtgccgaaga aacgcgtacc
    38221 cgccaatcca gaaaaagtgc tgaaactgac gggcgcacgc ggcaacaacc tgaaagacgt
    38281 gacgctgacg ctgccggtag gtctgtttac ctgcatcaca ggggtttcag gttccggtaa
    38341 atcgacgctg attaacgaca cactgttccc gattgcccaa cgccagttga atggtgcgac
    38401 catcgccgaa ccggcaccgt atcgcgatat tcagggactg gaacatttcg ataaagtgat
    38461 cgatatcgac caaagcccaa ttggtcgtac tccgcgttct aacccggcaa cctacaccgg
    38521 tgtgtttacg cctgtgcgcg aactgtttgc gggcgtaccg gaatcccgtg cgcgcggcta
    38581 tacgccagga cgtttcagct ttaacgtccg tggcggacgc tgcgaggcct gtcagggcga
    38641 cggcgtgatc aaagtggaga tgcacttcct gccggacatt tacgtaccgt gcgaccagtg
    38701 caaaggcaaa cgctataacc gtgaaacgct ggagattaag tacaaaggca aaaccatcca
    38761 cgaagtgctg gatatgacca tcgaagaggc gcgtgagttc tttgatgcgg tgccagctct
    38821 ggcgcgtaag ctgcaaacgc tgatggatgt tggcctgacg tacattcgcc tcgggcagtc
    38881 cgcaaccaca ctttctggtg gtgaagccca gcgcgtgaag ctggcgcgtg agctgtcaaa
    38941 acgcggcacc gggcagacgc tgtatattct cgacgagccg accaccggtc tgcacttcgc
    39001 cgatattcag caactgctcg acgtactgca taaactgcgc gatcagggca ataccattgt
    39061 ggtaattgag cacaatctcg acgtgatcaa aaccgctgac tggattgtcg acctgggacc
    39121 ggaaggcggc agtgggggcg gcgaaatcct cgtctccggt acgccagaaa ccgtcgcaga
    39181 gtgcgaagct tcgcatacgg cgcgcttcct caagccgatg ctgtaatcgt taaggccgct
    39241 ttttgagcgg cctcagatta ttgacaaagt gcgctttgtt cttgccggat gcggcatgaa
    39301 cgccttatcc ggcctacaaa aacatgcaat ttcaatatat tgcagagatt acgtaggcct
    39361 gataaagcgt agcgcatcag gcaattttgc gtttgtcatc attctcaggc cgctttctga
    39421 acggtctttt cctttcagag ttgcaccagt aatttacgtt tttcttccgg cagcaaattc
    39481 accgcctgct gataagacgc atccaccaga taatagattt gcgaatccgg cagcgaaccg
    39541 tcgagataca cggtgctcca gtgcgcttta ttcagatggc ggcttggacg cacatcgctg
    39601 tgctgctgac gtagcagctc cgccagctcc gggctggttt tcagcgaaac agctgggcga
    39661 ttttctactt ctttcaccat cgcaaacagc acatcttcca ctttgatctg cgtcgctttc
    39721 cagtcgttat gcacgctctg ttctgcgcct ggttttgcca tgcaatattg tagcaactcc
    39781 gaaatggtca ttttttactc cccttgaagt gtcgcgatga tgcgacgcga tccgccgtgg
    39841 atgcgatgtt cccccagcca gatgccttgc caggtaccgg tctgaatacg ccctttatgc
    39901 accggcaata caagcgatgt tcccagcatt gaggatttga tatgagaagg catatcgtct
    39961 gctccctcat agtcatgctc ataatttccg ttatcgggaa cggcgcggag gaaaaaacgc
    40021 tccatgtcgt ggcgtacggt gggatcgcag ttctcattaa gtgtcagaga ggcggaggta
    40081 tgttgcagca acagatgcag taagccgatg ttaacgcgcg gcatatcagc cagctgattc
    40141 agaatttcat ccgttaccag atgaaaccca cgagatttgg cgctaagcgt gagcgtcttt
    40201 tgataccaca tgtgctgctc cttgataaaa ctctcttaat cagtttgcag caagacagcg
    40261 aaaggataaa ggtgtgatta aaaaaacagc attcgggaga gtattacgct ctcccgctct
    40321 gtcagtattc tgaattgacg atcacctctt caccaaacgc acccgcttgt ggcaagggtt
    40381 tgtaggtaga gttggaggcg cgcagaatgc ggataccacg agcgccgaca tcgcgtgcgg
    40441 cggtaatatc gttatcggaa tcaccataaa aaattcggat atttttatcc tgcagccatt
    40501 gcgattttgt attttgccct agtttatcgc ccgcaaagat caccggattc atgttggtgg
    40561 caggaatatg aaaattatcc gccagcgttt ttgaaaccgt ttctgttttg gtcgggctac
    40621 gaccagtcac aaagaagatc gcgtcaccgc ggcgtacatg catatcaatc agctggcgag
    40681 cgacctcttt tggaatgctg aattcatccc agccattgtt cattttttcc cagaacacag
    40741 gatttttcag ataatcttcg ctttctggcg agaaggtttt tttgccgcgc cagaaacccg
    40801 gactggaaaa aagtaccgta tcatcgatat caaaccccac cgccattggc ggacgtcctg
    40861 cgaggctatt ttcaatttgt gcaaccgaaa cccaatgaat tggcgcctgt tcagcgagtt
    40921 tggctacgtt agtacccgga ttaagcggtg aaggagatga ggccagggca acagcggaac
    40981 tgtttagagc gaacaataag caaacggcac tcaatgcctg tgtgatcttg cgcatatttt
    41041 tccctaaata gtcagtaagt tgaaacttat tattgtgcgt ttgttgaaaa aaccatctga
    41101 ccataacgcc aaccgcgggg gaaaggaagg attatctgcg gatttgtcac ataaaaagag
    41161 catgatcaca tttggaatac ctgtgcgata aaccacacat tgccacggtt attctgctta
    41221 cacattcaca caatggcact cttttggttc tcgcaaacat tgttcaggga atatactgat
    41281 gataaatata aatgcattca atggatgatg aatgaaaata tcgtggaatt atatatttaa
    41341 gaacaaatgg cgatttcaca ttacaagcat ttcacttttt cttatcatgc tcgcggtttc
    41401 aatcgctttt ttgcacttgc gttttaatac cttgtccggt accgataaaa tgcggcttga
    41461 aatgtataag tccacattat attccaccat cgagcaattt tatgttttac cctatatgct
    41521 ctcaacagac catatcatcc gtcaggcggt aattacgcct gacgatatga cgtccagcga
    41581 actcaatcaa cgaattgcac atttcaatac tcaactcaaa accgcagcaa tatttattct
    41641 ggatacccaa ggtaaggcca tcgcttctag caactggcag gaccccggca gctatgtagg
    41701 gcaaaattat agctatcgcc cctattataa acacgccatg tctggcttaa atggacgctt
    41761 ttacggtatt ggtagcacta caaatacacc gggattcttc ctctctacaa gtataaaaga
    41821 taaaggaaaa attgtcggtg ttgtagtagt aaaaataagt cttaatgaaa ttgaaaaagc
    41881 atgggccgaa ggtcctgaaa atattatcgt gaatgatgaa catgggatta tatttttaag
    41941 ttcaaaatcg ccatggcgaa tgcgaacact gcaaccgtta cctgttcagg caaaacaaaa
    42001 actacaatct acccgtcaat atagtctcga caatctttta ccggcagatt attatccctg
    42061 ttataccgtg agcaatttta ctttcctgaa agataaaaaa gaacaactct gtttattccc
    42121 gcaatattat acacaacaaa tagccattcc agaatttaac tggaaaatga caattatggt
    42181 ccccttagat aacctgtact ggtcatgggc tatttcgtta gtcattacac taattattta
    42241 cctgctgttt ttgttattta ttaaatactg gagaatgcga tctcatgcac aacaattatt
    42301 aacactggcg aatgaaacat tagaaaaaca ggttaaagag cgtacatctg ccctggaatt
    42361 gatcaatcaa aaattaatac aggagataaa agagcgcagt caggctgaac aagtattaca
    42421 aattacgcgt agtgaactgg cagagtccag caaactggcg gcgcttggac agatggcaac
    42481 cgaaattgcc catgaacaaa atcaaccgtt agccgccatt cacgcactta ctgataacgc
    42541 gcgtactatg ctaaaaaaag agatgtatcc gcaggttgaa cagaatctga aacacattat
    42601 ttcagtgatt gagcggatga cgcagctcat ttccgaactt aaagcatttg cctcgcgcca
    42661 tcgcgtacct aaaggttctg ccgatgtcat caaagtgatg tatagcgccg tggcgttact
    42721 taatcacagc atggagaaaa ataacattga gcgacgaata aaagccccat ccatgccgtt
    42781 atttgtcaat tgcgatgagc tcggtcttga acagatattc agtaatttaa ttagcaacgc
    42841 cttagattct atggaaggta gctcttacaa acgactggat atcgccattc gccaggcaaa
    42901 taacaaagtt attattacca ttaaagacag cggtggcggt tttgcacctg aagttgtcga
    42961 tcgcatattt gaaccatttt ttaccactaa acgtagagga atggggttgg gactggcaat
    43021 agtcagcgaa attgtccgaa attcgaacgg cgcactccac gccagtaatc atcctgaagg
    43081 cggcgcagta atgacattaa cctggcctga atggggagaa gaacatgaat aataaatcca
    43141 gtgaaattac tatagtttat attgaagata gtgacgatgt acgttttgcc tgtgaacaaa
    43201 cgctcacgct ggctggttat cgtgttattt cctgttgcga tgcagaacac agtattcccc
    43261 ttattcaaag tcaggccaac attattatac tgaccgatgt caggttacct ggaatatcag
    43321 gactagagct cctcagttac atcaacgaaa tggatagcaa aattccggtt attttaatta
    43381 ccggacacgg tgacgtggaa atggcggtgg atgccatgcg taatggtgca ttcgatttca
    43441 ttgagaaacc aagtagctca gataaattat tgagtattat tgccagagcg gtagaaaaac
    43501 ggcgattggt tttagaaaat cagcaattac tggcgaatct gcaacaggaa aatggcccgg
    43561 tgctgattgg tcgtagtccg cagatgcaac aactgcgtaa aatgatcctt aatgtcgcag
    43621 ataccggtgc cgatgtcctt atttatggtg aaacaggttg tggtaaagaa gtcgtcgcca
    43681 gaatgctgca tcactggagt acccgccgtc aggggcagtt tgttgcctta aactgtgcag
    43741 gcttaccaga aacgttgttt gaaagcgaaa tatttggtca tgaggcgggc gcatttaccg
    43801 gtgcggttaa aaagcgtatt ggaaaaattg aacatgcaaa tggtggtacg ttatttctcg
    43861 atgaaataga gggaatgcca tcaggaatgc aggtaaaact gctacgtgta ttgcaggaaa
    43921 gaaccattga acgattaggt gccaatcaat taattccggt gaattgccgt gttattgcag
    43981 cgaccaagga ggatttactg cgtcgcagcg aagaacatct gtttcgtctc gatctctatt
    44041 atcgtctgaa tgtggttagc ctgaatattc cacctctacg ccagcgacga gaagatatcc
    44101 ctgaactgtt ttactggttc gccagtcagg cggcacaaaa atataatcgt cctctgcctg
    44161 atatttctcc catgttactg gcctggttgc agtcacaaag ttggcctggc aatgtacgtg
    44221 aacttaaaca caatgcagag cgttttgttc tcggtttact gacccaccac cagccagtgc
    44281 caatgactca gcaagaagag agtggtctta ccgcctgcat tgatgctttc gagaaaaagt
    44341 taatcgaaga tatgctgcga caaacggaag gccaggtcag tttgacagcg cggctattac
    44401 agctgccgcg caagacttta tatgacaaac tcaacaaaca tcagattcaa ccgcaagttt
    44461 atcgaccaga atcatccagc taatgctgat tcctgaggat gtaaccccaa atcgatacct
    44521 ttgcgggcgt aatgttcaac tacccaggac cagaatgata ccgggatcgt aatcatcttc
    44581 gcattgcgtt cccggttggc agcttctggc ataccggggt aaatcaccgg attgccgcca
    44641 ggttgaggct gcgtagctaa cagataatcc gcaaagaact gccctttctc ccctgccaga
    44701 tcggtgaatt gatcaggatc gataatcaac gcaaaaagat gattatgcgc agatccatcc
    44761 gtctgatgat cttcggcgat cgtgtcacca ccagataaga tccccgccat catttcaatc
    44821 atcagtgcaa ggccaaatcc tttatgctca ccaaatggca gtaacgcccc tggaggattg
    44881 ctgaataaca ccccaggatc attgcttgga ttcccctgtg catcaatcaa ttgtcccggc
    44941 gcggcctgct tcccggtgga cgacattacc cgtgctttat ttagcgccat gctactggtg
    45001 gcaaaatcaa ccagaatcgg cggacgccca tcactgaacg gccaggcaaa gcagaaaggg
    45061 ttagtggtca tacgcggttc cgcaccgccc cacggggcga ccagtgcagg acgcccggtg
    45121 acgttactca tggccagcat cgccaggcct tgatcagcaa ccatttgccc gtaatgcccc
    45181 atacgcccta aatgatgcgc atggcacaac gttaacaggc aaaaaccgcg gtgttttgct
    45241 tgttcgatgg cagcatttac cgccactttt ccggcgtgtt gaccaaagcc attgtgggca
    45301 tgaaaacgtt gtaaattacc ttcacttgtt aagcattccg ggcgggcatt cgcggttaca
    45361 tcgccacgag cgatattttc cagatatttc ggtagcagcg tgacgccgtg gctggcaaag
    45421 ccataacgtt ccgcttcaat catatgttcc gccacatcct gagcgatatc ggcatcacag
    45481 ccattaagtt ccagcagccc ggcagctaac caacgcactt tttcaacagc aatttgtaat
    45541 gacatcgttg gtttcctcac attagaccta aaattttcca ccaggtggcg gcaaacagta
    45601 gcaacaacag gtagccgatc accgtcaggt acagtcccac acgggtaaac tggcgtggcg
    45661 tgaaggtatc cgtacccata cacaccatat tttgtggcgc gttaatcggc aggatgaagc
    45721 caaaactgac actgaaggcg agcagaatgg tcatgccgac cgggttaacg ccgagttctg
    45781 gcggcagact gcttaacagg ctaatcagga tgggcagcaa tgcggcagtt agcgcagtgg
    45841 cactggcaaa ccccaagtga ataattatca agaaagcggc gagaatcgcg aagatggcta
    45901 atgatggcaa gccatcgaga ccaaaacctt tcaccacata gttcgccatc catgaagctg
    45961 cctgcgtatc aagcagcgtg gaccccaggc tgataccaat accaaacata agcaacgtgc
    46021 cccactgcac acgtttttcg acctctttcc agctcattac accgatgcct ggcagcaaca
    46081 taatcgccag tccggcaagg gtgacagagg tggtatcaat actgtgtaat tttccgccag
    46141 tagaccaaaa gagcagtaac aatagcgaaa taccgatcaa acgtttttct ttaccagtgg
    46201 ttggccctaa ctctgcaagt gcttttttaa tggcctcgct ccccccttca acagcttccg
    46261 tttctggcgg caacaattta cgtgcgagga aatagaggat cgccgacatg gtgaggctcc
    46321 agggcgcgcc cgctaacagc cagtcaagcc agctgacaga atgcccggcg ccgaaagttt
    46381 tattgataaa accgatagaa agcaggtttt gcgccgccga agtttgaata ccaacgttcc
    46441 agatactggt cgcctgggcg ataacaatca tcattgacgc cgctaaacgt gaatgtttat
    46501 ccaccttaaa tgccgcaatg acccccatca taatcggcac cacgcaagcc gtacgggcag
    46561 tggcactggg tacaaccagg ctcaggacga tggtcaccac aatggcaccg ataataatgc
    46621 tgcggctgct ggcacctatt ttcgacatag taaacagcgc aatgcgttta tccagcccgg
    46681 taatggtcat tgccgccgcg ataaacattg ctgctgccac cagcgccaga gcagaattag
    46741 aaaaacccga cagagtcatt ttcagcgctt tcgcggtgcc aaggatcgtg tccgggtgat
    46801 tcatatccgg ggcaaaacca accataaaga taatcagtgc ggaaataaca attgcactgg
    46861 cggtgtaatc catcgcttcg ctgatccaga caataatggc aaacaccaga atggcgatca
    46921 tattttgtcc agcgacgggc aaggtatcgc ccagtggcag aagggtaata ataattccgg
    46981 caatgatcgc caaccacagg ccgggattag ggataattcg acgcttttgt ggtgcgccat
    47041 gggatattga tgacatattc acctcattgt gaaaaataaa aaacctgcct ggctattgca
    47101 ggagctatgc catctttaat tttttaataa cgctttgtta tttaatgatt ttattattta
    47161 ttctttttat tttgtgcgcg gttttccgca cagtaaataa aaggtgattc tcttaccaca
    47221 cagataaaaa aagggagcga tatgctccct tgatattcat ttatggatta cttaataaca
    47281 ttccataacg tttcgccaag caacgccggg tttcttgtcc atgcctgctt tatcgaacgc
    47341 acggaattta tcctctgcac tgccgctacc gccagaaata atggctccgg cgtgccccat
    47401 gcgttttcct tttggtgcag taacaccggc aatataacct acgaccggtt tattcatttc
    47461 atcacgaata aattccgcgg cccgttcttc cgcattacca ccaatttcac cgatcataat
    47521 taccgcgtct gtttgcggat cattttcgaa taaacgcagc gcatcaataa agttagtccc
    47581 cggaaccgga tcgccgccaa taccaataca ggtggattgc ccaagtccca gcgccgtggt
    47641 ttgcgctaca gcttcgtagg ttaatgtacc ggagcgggaa acaatcccaa tacgccctgg
    47701 gcggtggatt tcaccaggca taatcccgat tttgcactcg cccggcgtga taactcctgg
    47761 gcagtttggc ccaatcagcc gcacatccgg gtggcagtca agcagccgac gaactttcac
    47821 catatccagt gtcggtacac cttcagtgat caccacaacc aatttgacgc cagcttcaat
    47881 agcctcaaca acagaatcca gcacaaacgg tgcaggcaca taaatcacgc tggcatcagc
    47941 ctcagtttgt cgcatcgctt ctttcattga atcaaaaacc gggcgatcca gatgccgttg
    48001 cccgccctta cctggcgtca ctccacccac aatctgcgtg ccatacgcaa tagcctgctc
    48061 tgtatgaaat gtgccgtttt tgccggtaat gccctgcacc agtacccgcg tgtgtttgtt
    48121 gatcaaaacg ctcatcttaa cctcctgtta attcagtaac gcaatgatgc gtttagcggc
    48181 atcatccaga gaattgacgg cttccaccgt caggccactt tctgccagta accgctgccc
    48241 ttcggcagcg ttattaccgg agagacgcac cacgacaggc agggtgatac gggcctcatt
    48301 cagagcatga ataatggctc tggcgatcat gtcgcaacgg acaatcccac caaaaatatt
    48361 aaccagaatg gctttcactt tgctgtcgga aacaatcagc cgaaaggctt cactgacgcg
    48421 ctcctgggtt gcgccaccgc caacatccag gaagtttgcg ggttgttcac cgtatagctt
    48481 gatgatgtcc atggttgcca tagccagccc tgcaccattg accatgcagc cgatatttcc
    48541 gtccagcgag acataattaa gatcaagctt cgctgcctgg ctttcgcgtg gatcttcctg
    48601 ggtttcatca cgtagcacct ggagttccgg gtgacgatac agcgcgttat catccagcga
    48661 tactttcgcg tctgcacaca taaattcgcc cgtttcccgc aacaccagag ggttaatttc
    48721 cagcagggcg aaatccagtt cgttaaaggc tttccacgcc tgattaacca ggcgactaaa
    48781 ggcagcaaat agcccgtgtt ccagttgcag aacagcaaac atttcgcgga tatggcaagg
    48841 ctgcacgcct gtcagtggat caatgctgac actgctgatt ttctccggtg tctcatgggc
    48901 gactttttca atttccacac caccttccgg gctgacaata aacgtgaccc gctggctttc
    48961 acgatccacc accatgccaa aatagagttc ctggcgtacc ggatagatgt tttcgcacag
    49021 caaaatactg ctgacatact gcccttctgg cccggtctga taagtcacca gttgcgatcc
    49081 caacatttgc tgtacaaaag cctgtgcttc cggcagctgc ttaagcactt ttacgccgcc
    49141 agctttaccg cgcccaccag catgtacctg cgctttaagt accgcgcctt tactcgggca
    49201 agcaatatgt tgccaggcat ctgccagttg cgaaatctgt tggatggcta tctcttttgg
    49261 acacggcatc cccatgccag ccagtaacga tttggcctga tactcatgta aattcatata
    49321 ttccctcagt ggtatgtgtt atccgtgtaa tgctttatta ttcaaggcca ttgctgcttc
    49381 gtggatgact tcgcttaagg tcggatgcgc atggatcgca caggcaatat cctcgccaga
    49441 ggcgctaaaa gtcatcgcca gcgcaatttc attgatcagc tccgatgcct gcgggccaac
    49501 aatcgctccg ccaagtacgc ggtcagtgtg tttatcgctg tataacgtgc aacgcccacc
    49561 ttcctggcct aatgctaagg cgcgaccgtt accggcaaac agagaatttc ctttgttaaa
    49621 gactctgcca gcggctttca gtgaggcctc gttttcccct acccacgcaa cttccggttg
    49681 ggtgtaaatc accgaaggga tcagcgcaaa attaatcggc tctaccgcca gtccggcaat
    49741 ttgatccgca accaccacac cttcagccat cgctttatgt gccagcatcg gccctcgcac
    49801 gacatcacca attgcccaca gcccggcttt cccggtacgg cataagttat cgacggcaat
    49861 accgccacgg ttgtcggctt caagaccgag ttgcacgaga tcgacaccag aaagtcgcgg
    49921 tacacgtcca atggcaagaa ttaatttatc gaagcggctt tcttctcgct tctcaccctg
    49981 ccgccagcgc acatgcacac cgtcatcccg ttgttcaata gcctcaatct ctactgccag
    50041 ttgcattttc atcccactgg caatcattgc tttacggact tcgttcgaca ggcgagcttc
    50101 cagtgcaggc aagaacgttg gcgccatctc cagcaacgtg acgtctgaac cgacccgatt
    50161 ccacacagac cccagttcaa ggccaatcac gccagcgccg atcacaccga gacgcggcgg
    50221 cacagcggtt aacgccaggg cgccgcgatt atcgagaatt tgctgattat caatggtaac
    50281 gccaggcagt tgacgcggct gagaacctgt tgcaatcacc acattacggg cgcggatttg
    50341 ttgatcgttt acacgtaatt gccaaatctc atcccgagca tgttctaacg ttgcgaggcc
    50401 gcacaggtac ttcactttat ttttcttaaa cagcaagccg atgcccattg tcaggcggct
    50461 tactatggcg tctttacgtt gaatcatcgc agctgcattg aaagatacgc cgtcaacatt
    50521 aacgccatga atgctcgcct catgctgcac ttgcgcgtac agttcggaag attgcagcaa
    50581 cgattttgac ggaatacagc cgacattgag acacgtaccg cccggcgaag gttcgccctg
    50641 tgcattaaca ccatcatcaa tacatactac ggaaagccca ttctgcgcag cccgcagtgc
    50701 cgctacgtaa ccacctggcc caccccccat aactgccaca tcaaaaattg tactcatcgg
    50761 cgccctcctt aaagatccag caacagttgt tccggtgatt ccagtagctc acgtattgcc
    50821 accaatgtct gcacggcttc ctgaccatcg ataatgcgat ggtcatagct gagagcaagg
    50881 tacatcatgg gacgaataac aacctggccg ttttctgcga ccgggcgcgg cgtgatggca
    50941 tgcatcccaa gaatggccga ctgcggcggg ttaatgattg gcgttgacat catcgaacca
    51001 aaagtaccgc cgttggtgat agagaacgtg ccgccctgta acgcctccag cggcaatttg
    51061 ccattgcgtg cctgtgtggc gtattcggca atttgccgtt caatttccac caaagagagt
    51121 gattgcgcat tgcgtaacac aggcactacc aggccgcggt tgctgctcac tgcaatgccg
    51181 atatcgcaat aatcacgcca gataatctcg ttgccatcaa cgctggcatt cactacaggg
    51241 aagcgctcca gcgcccgggt aacggcctta acaaagaagg acataaagcc cagcttcacg
    51301 ccgtgcttct cggcaaaacg atctttccag cgggcgcgta aatccatcac gctctgcatg
    51361 tttacttcgt tgaatgtggt aagaatggcg ttattttgtt gggaagccag cagcaatccg
    51421 ctgacgtaag cgcgacatcg gctcacgacg ttcctgacga gcacccggtg tcaagggttt
    51481 agctggcgcg atctccgcca cccgttcagg ctgaatgact ggtgcaggag taacgcgctg
    51541 tacatcctct ttcaggatac gtccatttct cccgctcccc gcaacgtcag caagttcgac
    51601 accactacgc tgtgcctcca gccgtgcgga aggcatcgcc agggtttcgg tgactggtgt
    51661 gacggtttct tctatgacag cttgcggctt aagatgcgcc aacagttgcg cggacgtgac
    51721 cgtgctacct tcgctgacga taatattgct taacacgcca tcgtgcgggg ccggtatttc
    51781 cagaatgact ttatcggttt ccagctcggc aatcacatca tcgcgtttta cgtgctcgcc
    51841 ttcttgttta caccaggttg tcagcgtgcc ttccgtgaca gattccggta atacagggac
    51901 agtaatttcg atcataaaat gttctcctta ttatttttgt gcgcctttca tggctgaata
    51961 tctgcaaacg cagcagcaag aaattcgtcg atttgttgtt tatgtacacg cccataccct
    52021 gtcgccggag ctgccgctgc cgggcggcct gcatactgcc agtaaggagt gttaattttc
    52081 agtgccgcca gctcatgacg aatctgccgc cacgcgccct ggttttctgg ctcttcctgc
    52141 aaccaaatcc attcgcagca gttcggccaa ctcgccagaa catcattcag ttctgcgacc
    52201 gggaacgggt agagttgctc caggcgaaca atcgccactt cgtcttcatg ctcacgctgc
    52261 ttacgggctt ccagtacgtc gtaatagacc tggccgctac acaaaataac gcgcttaact
    52321 ttctgggact gatgcaattg cggatcggtg attaccggtt tataagcgcc atcagtgaat
    52381 tcacaaagct cgctcatagc gcctttaaaa cgaagtaaac gcttactcat cattatcact
    52441 aacggtttgc gcattgggcg taatgcctga ccgcgcaaca aatggaacat ttgtgctgac
    52501 tcactgggca tcaccacctg catgttgttt tctgcgcaca gttgcagcca gcgctcagga
    52561 cgcgcagaag agtgctccgg cccttgtccg tcatagccat gcggcagaag aatggttaaa
    52621 ccgctatagc gatcccactt ggtttcgcca gacgagataa actggtcaat agcaacctga
    52681 gcgccgttgg caaagtcacc aaactgtgct tcccaaataa ccagttgctg aggcgcagac
    52741 gttgaatagc catattcaaa ggctaagagc gcttcttcat taagcaccga atcataaaca
    52801 tcgaaagatg cctgaccagc gcggatatgc tgtaacggca gataacgacg ggcttcagtt
    52861 tgatgatgta ccactgcatg gcgatgactg aacgtgccac gccctgagtc ttcaccagaa
    52921 agacgcacac caacacccgc atccacaagc gatgcatagg ccagcatttc agccattccc
    52981 cagtctattg gctgagtacc tgcggccata tcctggcgta acgccagttg ccgtttaatg
    53041 gtaggatgtg cgaccacatc gggtggcagt gtagaaatga tttcaccata cgctgccagc
    53101 ttttgccgag gcaatgccgt agataccggg gcgctccagt gagggttggt caacccatac
    53161 cagttagcgc taaaagagtg aattgcaggt ttgagaggct gcggttcacg tttctggcag
    53221 ctatccagcc agtcgcgata gcgtgccgtc atctcatctt gttgcgcctg agttaacagg
    53281 ccgcggcggg caagagattc accataacgg gcgagcgtac cagggtgagc atccactgcc
    53341 tgatacattt gtggctgggt aagacgtggc tcatcgcttt cgttgtgtcc gtgcttacgg
    53401 aagcagcaaa tatcgatgat gatgtcgcgc cggaaggtgt ctcgccattc gcacgccagt
    53461 tccattacct ggcaaacagc gtcaacgtca tcgccattaa cgtggataat tggggccgct
    53521 accattttgg caatatctgt gcagtaccgt gaagaacgca tatcctgcaa gcgggaagtg
    53581 gtgaagccaa tctgattgtt aatcaccaga tgtagcgtac cgccagtgcc gtagccctga
    53641 gtctgcgata agttaaacgt ggtttggttg acgccgaggc cgcccaacgc ggaatcccca
    53701 tgaataagta cgcccaccac ttttgcctgg ccatcctcac cacggcgttc ctgcctggct
    53761 cgcacctggc ccagcaccac cggattcacg atctccagat gtgaggggtt atacgccagc
    53821 gccacatgca gcgaaccggc aggtgtttcc agattgctgg aataccccat gtggtatttc
    53881 acatcgcctg aaccactgcc gatcgtctgc ttgccttcaa actcagcaaa aagctgtgcc
    53941 gggtctttat tcaacagatt aaccagaaca ttaagtcttc cccggtgcgc catgccaatg
    54001 accatttcct ccacaccttg cgcacgcagg cgttttgtca gcgtatcgag cgccgggatt
    54061 gcactttcgc ccccttccag agaaaaccgt ttctgcccaa cataacgggt atgcagataa
    54121 cgctctaacg tctctgccgc catcagtttt tcaaaacgag caatacaggt ttgtacatct
    54181 gcttgtggcg cattgctgct ctcaatccgt gataacaacc agttgatttc ttcacgattt
    54241 tccagatgag caagttcata agcctggcta cccgcccagg cttgctctag caggttaagc
    54301 agttgcttaa gtggcatttg cgtggtgtgt gcgccaaagg tgacgctaaa ctcctgcaac
    54361 aagtcttctt cgcttaatcc ccaaaaacca ggcaggaggg agggaacgtc agccggggga
    54421 ttcaaaccaa gtggatcaag ctttgcgcgc agatgcccct gcgtgcgcca ggcattgatc
    54481 agttgtatta tcgcagcctg tttttttaag gtcgcaccgc tgacgttgtt atcaccggat
    54541 acgctttgga cagtcagttc aggtgaaaga aaaaaacgcc gccagtcttc cgggagttgt
    54601 tccggcgtac gcaaccaggt ctggtagtaa tcttcaagcc aggtcgcgtt atctccacta
    54661 agcaacgtgc cgggcgaggt catgttctcc atgtttttct cctgatggtc atagacagcc
    54721 gcattactgc acaatccgta ccaacagtta aaaacactaa ttttcaaaaa cttatagaaa
    54781 atagatgatg taaaagtggg cgaaaaaagg gacagaatag taggggatgg ctggagtcag
    54841 gaacaggcgg agagatccgc ctgttgatta aaatcacatc accgcagcaa acgcctttgc
    54901 cacgcgatgc acattttgcg cgttaagccc ggcgacacac atgcgaccgc tggcgatgag
    54961 atagacacca aattcttcac gtagtcggtc aacctgagcg gttcttaaac cggtataact
    55021 gaacatgccg cgctgattaa gcagataatc gaaattgcgc tctggcatct ctgtgctcaa
    55081 taccttcacc agttcctgac gcattgccag aatgcgagtt cgcatctctt ctacttccgc
    55141 cagccagctg gctttcaatg cctcgtcatt cagcaccgca gccaccacct tcgcaccaaa
    55201 attcggcggg ctggagtagt tgcggcgaac ggttgctttc aattgcccca gtacgcggcc
    55261 tgcggcttcg gcatcttcac acagaacaga aagtccgccg acgcgctcgc cgtaaaggga
    55321 gaaaattttc gagaacgaat tgctcaccag agcgggtaat ccagcgctgg caatggcgcg
    55381 aatggcgtag gcatcctctt ccataccggc accaaatcct tgataggcaa tatcgaggaa
    55441 tggaataagc tcgcgggctt tgagaatttc aatcacggaa tcccactggt cattagtgag
    55501 atcggcaccc gttgggttgt ggcaacatgg atgcagcaac acaatactgc gggcaggtaa
    55561 tgttttcagc gtcgccaaca ggtcattaaa gcgcacgccg ttagtcgctt cgtcatacca
    55621 ggggtaagtg ctcacttcga atccagcccc ggcgaatatt gctacgtggt tttcccaggt
    55681 aggatcgctg acccagacgc ctgattccgg gaagtagcgt ttcaggaaat ctgcacccac
    55741 tttcaatgcc cctgagccgc caagggtttg aatggttgct acgcgctgtt gttgcagtac
    55801 cggatggtcc gcaccaaaca gcagcggcgc aatggcatgg cgatagctgt taagcccttc
    55861 catcggtaaa taaagcgaag caccatgagg ctgcgcattc aggcgcgctt ccgcatccgc
    55921 cacggctttc agttgtggaa taattccgtc ttcgttgtag tacagaccga tacttaaatt
    55981 cactttgtcg ctgcgagggt cttctttaaa acgctccata agcgtaagaa tcgggtcgcc
    56041 agcgtaggcg tcaacttttt gaaacacgcg atggttctcc aggtttacgg gcaggtggtt
    56101 aaaacacaat aaaccggaag taggcgaaga tcgagaggat gttcaggggc gaatggcaat
    56161 tagcaacaga gtgagactca tgacaaacgt acatccgcca gatgcacgac ctttataaga
    56221 gcatgaaaga atatcaactc attgaattgg taagatttta ttggccggat aaggcattca
    56281 cgccgcatcc ggcacagaca atcaaatatt acagaacgat taatccacgt atttcatcgc
    56341 gactcttgag gtcaggcgcg tgataagttc gtaagcactt actttcgtca tttcagcgat
    56401 acgttctacg ggcaaacctt cgccccataa aatgaccggg tccccggctt tatcctgcgc
    56461 ctgtggacct aagtctacgc agatcatatc catcgccacg cgcccgacaa tcggcacttc
    56521 gcgaccgttc accagcactg gcgtaccgga cggcgcggcg cgcggataac catcgccata
    56581 gcccatcgcg actacgccaa gacgagtatc acgttcgctt acccaggttc caccataacc
    56641 gacaggctct ccggctttat gctcacgcac ggcaatcagg ctggaggtta gcgacatcac
    56701 tggctgacag ccaaaatcgg caccggtgga gcgatcttca agcggagata cgccgtaaag
    56761 aatgatgccc gggcgcaccc agtcaaaatg cgactgtggc cagagtaaaa tgccacccga
    56821 cgcggcaatg gaacgttgac caggtttgcc ttcgcaaaag gtattaaaga tagcgagttg
    56881 cttctcggtt gcgccgcatt ttggttcatc cgcgcgcgca aaatggctga cgatattcac
    56941 cggctgacga acgtttttgc actgggtcag acgatgataa aacgcctcag cctgttccgg
    57001 ccttacgccc agacggtgca taccggtatc gagtttcatc cagacggtaa ccggctcgtc
    57061 caggctagcc tcttccagcg cagccagctg ttcttcgtta tgcacggcgg tatgaaaatg
    57121 ttgcgcagaa atcgtcggta gatctctggc atcaaaaaag ccttcgagta aaagtacagg
    57181 tttggtgatt ccccccgcac gcagtcgcag agcttcttcg agacgggcta cgccaaaggc
    57241 gtcagcatcg gggagcgttc gcgcggtctc aagaagaccg tgaccataag cgttcgcttt
    57301 caccaccgca accattttac tggcaggcgc cagttcacga agacgttgca ggttgtgtcg
    57361 cagagcgcgg cggttaatca caacagttgc cgcttgcatt tgtgttcctt gataagtgtt
    57421 tgctttaatt acctaattca taaaataatt attattcgtc gtcgtactgc ggccccgcat
    57481 agttgtcgaa gcgcgaccat tgaccgttaa aggtcaggcg taccgtcccg attgggccgt
    57541 tacgttgttt accgataata atttccgcga tgccttttaa atcactgttt tcgtgataca
    57601 cctcatcacg atagataaac atgatcaagt ccgcatcctg ctcgatagag ccagattcac
    57661 gcaggtcgga gttgaccggg cgtttgtcgg cacgttgttc cagagaacgg ttcaactggg
    57721 acagcgccac caccggcacg ttcagttctt tcgccagtgc tttcagcgag cgggagattt
    57781 ctgcaatttc cagcgtacgg ttatcggaaa gcgccggtac gcgcatcagt tgcaggtagt
    57841 cgatcatgat aagcccgatg ccgccgtgtt cacgggcaat acggcgtgcg cgggaacgca
    57901 cttccgttgg cgtcaagccg gaggaatcat cgatatagat attacgtttt tcgagcaaaa
    57961 tacccatggt gccggaaatg cgcgcccagt cttcatcatc gagctgcccg gtacggattt
    58021 tagtctggtc aacgcgcgac agcgacgcca gagaacgcat catgatctgt tctgatggca
    58081 tctccagcga gaagataagc accggtttat cctgcaacat cgccgcgttt tcgacgaggt
    58141 tcatcgcaaa tgtcgtttta cccatcgacg gacgcgcggc aacgatgatc aaatccgacg
    58201 gctgcaagcc agcggttttt ttgttgagat cgtcataacc ggtatttacc ccggtaacgc
    58261 catcgtgtgg ctgctgaaac aactgctcaa tacgcgccac ggttgcgtcg agcacatcgg
    58321 cgatgttctt cggcccttcg tctttgtttg cacgactttc ggcaatttta aagacgcggg
    58381 attcagcaag gtcgagcaga tcttcactgg tacgcccttg tggatcaaaa ccagcttcgg
    58441 caatctcgtt agcaacggag atcatctcac gaacaacggc acgttcgcgc acgatatccg
    58501 catatgcact gatgttcgcc gcacttggcg tattttttga cagctctgcc agataagcaa
    58561 aaccaccgac gctatcgagt tgcccctggc gttccagcga ttccgcaagg gtaatcagat
    58621 cgataggact accgctttcc tgcaaacgcg ccatttcagt aaagatatga cggtgtgggc
    58681 gggtgtaaaa atcgtctgcc accacacgct cggctacatc atcccagcgt tcgttatcca
    58741 gcattaaacc gcccaacacc gactgctccg cttcgatcga gtgcggaggc acttttagcc
    58801 cggcaacttg tggatcgcgt tcgcggggtt cagcctgctg tttgttgaag ggtttatttc
    58861 ctgccatagt gaatggagtt accgagataa agaatgggtc gaaactttac catatgaagc
    58921 agaccctgac gatacgttct ggaggacaca tggcaacacg aattgaattt cacaagcacg
    58981 gtggcccgga agtacttcaa gccgtagagt tcactcctgc cgatccggcg gagaatgaaa
    59041 tccaggtcga aaataaagcc atcggcatca attttatcga cacatatatc cgcagcggcc
    59101 tttacccgcc accatcgtta cccagcggat taggtactga agcggcaggc atcgtgagta
    59161 aagtcggcag tggtgtaaag catattaagg caggcgatcg cgtagtctat gcacaatcgg
    59221 cattaggcgc ttacagctct gtgcataaca ttaatgcaga taaagcggcg attctgcctg
    59281 cggcaatttc ttttgagcaa gctgcggcat ccttcctgaa aggcttaacg gtttattatc
    59341 tgctgcgcaa aacctatgaa attaaacccg atgaacagtt cctgttccac gcagcggctg
    59401 gcggcgttgg cttgattgcc tgccagtggg caaaagccct tggcgcgaaa cttatcggca
    59461 ccgtaggaac cgcgcaaaaa gcgcagagtg cgctaaaagc gggcgcgtgg caggttatta
    59521 actatcgtga agagaatctg gtcgagcggt taaaagagat caccggtggt aagaaagtgc
    59581 gcgtggtgta cgattcagtg ggcagagaca cctgggaacg gtcgctggat tgcctgcaac
    59641 gccgcggctt aatggtgagt tttggcaact catcaggtgc ggttaccggt gtgaacttag
    59701 gcattctcaa tcagaaaggc tcgttgtatg tgacacgccc ttccctgcaa ggctatatca
    59761 ccacgcggga ggaattaacc gaggccagta atgaactgtt ctctttgatt gccagcggtg
    59821 tgattaaggt cgatgtcgcc gagcagcaga aatatccgct gaaggatgcg cggcgtgcgc
    59881 atgagattct ggaaagccgg gcgacgcagg gttccagcct gttgattcca taaaagaaat
    59941 tgggcttcca cctgggaagc cctttctttt atagttcggc tgtatgtagg gtacagctcg
    60001 atgaatttgt ttgacgcgca atagtgacag atttgattat caattcctat tttgttctaa
    60061 ggataaaacc ttaggttgtg atcatccgca caatccctta gtaacgccag cggtcataac
    60121 gctgatattt cggcactttt ggtgctttaa tcgccctgat cacccacacc accgcaaccg
    60181 ccaacagcag ccacggcagc aacttaatca tcaatgccag cataccgccg aggaacataa
    60241 aggccgtcgc cacaatcagc gcggcgataa tacccagcaa cgaaacaccg gtgaccatca
    60301 gcatgacaaa aaagccaatt acaaaaagta gttccagcat gatgctctcc caaatatgaa
    60361 atctcttgct ggcattacaa gaatcatgcc aaaaataatc tattgattta acagcaaaac
    60421 gccccgcaac ggtgcgcagg gcgtggtgaa tttgactact ttttggtgaa aagttaacgc
    60481 ttatccgcca ccagtttgag cgcgtgttcc agcacattaa tgtctgcacc cgctttatgg
    60541 gcattttcac ttaaataacg ccgccactgc cgcgcgccag gaataccctg gaacaagccc
    60601 agcatatgcc gggtaatatg gccgagatac gtaccctggc tgagttcacg ctcaatgtac
    60661 ggatacatgg cgcgcactac cgccaccgga tcggcatcgg tatccgagga accaaaaatc
    60721 tctcggtcta ccgccgccag aatccccgga ttctgatacg cctcgcgccc gaccatcacg
    60781 ccatccatat gttgcaaatg cgctttagct tcttccagcg acttgatacc accgttaatc
    60841 gacatcgtca gatgcggaaa gtcacgcttc agttgataca cacgcggata atcgagcggc
    60901 gggatctcac ggttttcttt cggacttaac ccagaaagcc aggctttacg tgcgtggatg
    60961 atgaacatct cacactcacc tttgccggaa acggtgttga tgaaatcgca gagaaattca
    61021 tagctgtcct gatcatcgat gccaatacgc gtcttcaccg tcaccggaat cgacaccaca
    61081 tcgcgcatcg ctttcacgca gtcggcaacc agctgcgcat tacccatcag acacgcacca
    61141 aacatgccgt tctgcacccg gtcagacggg cagccgacat tcaggttgat ctcatcgtat
    61201 ccacgcgctt ctgccagctt cgcacactgt gccagcgccg ctggatcgct acccccgagt
    61261 tgcaacgcta ccggatgttc ttcttcactg tacgccaggt aatcaccttt accgtgaata
    61321 atcgcccctg tggtcaccat ttcggtatac agcaacgtat tgcgggaaag cagacgcagg
    61381 aaataacggc aatgtctgtc cgtccagtca agcatcgggg caatcgagaa acggtaagcg
    61441 ggataaacag tgtgtgattc tgctggcatg gcgacttttt aagcatctgg taaattgggg
    61501 gcgctactat agcataacga atatgcgatc aggcaatgtg acagccatga caccctgttc
    61561 tcggcgttta agtgagaact atggtaaagt aaggacattc ttaaccccac tgtcgaggtg
    61621 cccaatggaa aagaccacaa cgcaggagtt attagcgcag gctgaaaaaa tctgcgcaca
    61681 gcgtaatgtg cgcctgaccc cacagcgtct cgaagtgttg cgcctgatga gtctgcagga
    61741 tggcgctatc agcgcttatg atctgcttga tttgctgcgc gaagctgaac cgcaagccaa
    61801 gccgccaacg gtttatcgcg cgctggagtt tctgcttgaa caaggttttg tgcataaggt
    61861 ggaatccacc aacagttatg tgctctgtca tctgttcgat cagcccaccc atacttcagc
    61921 catgtttatt tgcgatcgct gcggcgcggt gaaagaagaa tgtgctgaag gcgtggaaga
    61981 catcatgcat acgctggcgg caaaaatggg ttttgccctg cggcataatg ttattgaagc
    62041 acatggatta tgttcagcat gtgtagaagt agaagcgtgt cgtcatcctg aacagtgcca
    62101 tcatgaccac tctattcagg tgaaaaagaa acctcgctaa aagggtgtac atccttgtac
    62161 atgtcgggca ggagggatta attaccagcg gtaatcatgg cgtttttccc aactatcaac
    62221 ctctttttct gcctgatctt tctgataacc gtagcgttcc tggattttac ccactaactg
    62281 atcacgtttt ccttcaatga tcgtcatatc atcatcggtc agtttgcccc attgctcttt
    62341 cactttacct ttaaactgtt tccagttacc gccggcttca tctttattca taatcaagac
    62401 ctcatcgtta ggttgtgaat gagagtacgt tcacttttct tctgaacgtg agattaagtg
    62461 tagtcagcaa tttggcttag gattatttat tcagaatttt taaccgtcac gttgcgacaa
    62521 accaggtatc gttacgccag tgacgccgcc agatagccgc cagagaaagc ccgcgtaacg
    62581 ccagaaagac ggttaacgcc agccacaaac catgattccc cagccacggc agcgtaagga
    62641 gcgtcagtgc aaaacctgcg gcggccaccg ccatactgtt acgcatttcg gcggcacgcg
    62701 ttgcgcctat aaacatgccg tccagcagat aacaccagac gccgaccaac ggcaaaatca
    62761 cctgccagat aagatagcgg tcagccagct gctgaatctg ggttaacgac gtcagcaacg
    62821 caatgatgtg ttcccctgcc agcaaataaa ccaccgaaaa cagtaacgct acgatccccg
    62881 actggcggca cgctgcccgc cagacatcca gcaattgact accgtcgcgc gcaccgtatg
    62941 cctgaccgga atgtgcttca accgcgtagg caaaaccatc cagcgcatag gcggtaaagg
    63001 tgagtagcgt catcagaacc gcgttaacag cgataatgtc actccccagt cgcgcgccaa
    63061 gtacagtgat cgcgccgaaa cagagttgca gcaacagcga gcgcagcatg atatcgcggt
    63121 taagcgccag caagtgacgg aagttacctc gccaggctgt tttcagcatt tcgccggaga
    63181 tcccgcgtag tttgaggatt ttacgcacca ttagcagacc aatcagcaat gttgcatatt
    63241 ccgcaataac cgttgccaga gccgcgccct gcacgttcat atgcagcccc atcaccagcc
    63301 agacatccag cacaatgttg aggatattgc cgaccactaa caaaattact ggcgcacggg
    63361 catattgcac gccgagtaac caaccaagta ataccagatt cgccagcgac gccggtgcgc
    63421 ttaaccagcg gatttcaaga aagcgtcgcg cctgttccag gactgcttca ctaccgccaa
    63481 caatatgcag cgccagatcg ataatcggcg tacgcagcag cgcaattaac gccccagccc
    63541 ccaacgccaa cagcaacggt tgcaccagcg cacgggctaa tgcctgagga tttttagcac
    63601 cataagcctg tgcagtcagc ccggtggtgc tcatgcgtaa aaacagcaac aacataaaga
    63661 gaaagctggt cgccgttgca ccaaccgcca cgccgcccaa ataaaccgga ctatcaagat
    63721 gaccaattac cgccgtatcg accagtccca gcaacggaac ggtgatattg gagaaaatca
    63781 tgggtaaggc gagatgccag agtgctttat cagatgaagt gaggaatgcc atgcagacaa
    63841 gcctgatgaa gagagatgaa aaacaaaccg cgacgccagg cggcatcgcg gtctcagaga
    63901 tatgttacag ccagtcgccg ttgcgaataa ccccaaccgc cagcccctca atggtgaagc
    63961 tctgctgacg aaggtcaacg acaattggtt taaactcgct gttttctggc aacagttcga
    64021 ctttattgcc ctgttttttc aggcgtttaa cggtaacttc gtcatcaata cgtgcgacaa
    64081 cgacctgacc gttacgtaca tcctgagttt tatgcactgc cagcaaatcg ccatccataa
    64141 tgccgatatc tttcatcgac atcccgctga cacgcagcag gaaatcagca ttcggcttga
    64201 acaaggaagg gtcgacctga taatgacctt caatatgctg ttgcgccaga agcggttcac
    64261 cggcagccac acgacctacc agcggcaacc cttcttcctc ttcctgcaat aaacgaatcc
    64321 cgcgtgatgc gccggaaaca atttcaataa cgcctttgcg tgccagcgcc ttcagatgtt
    64381 cttcagccgc gtttggggaa cggaacccca aacgctgcgc gatttccgca cgcgtcggcg
    64441 gcatacctgt ctggctgatg tgatcacgga tgagatcaaa cacctcttgt tgcctggccg
    64501 ttaacgcttt cattccgccc cctgggtgta tatacagtta tgctgtgagt atatacagca
    64561 aaaggcgatt ttggaaccat aaactgcaca ataaaccaga gatttatcga aatctggaag
    64621 ggttatccaa aatgcgacca taacaggatg caccaggtaa tcacggcgac gataatggca
    64681 atcagcactg cggcggatcc catatcttta gcgcgtccgg aaagctcatg gtattcagag
    64741 ccaattcggt caaccaccgc ttcgatggcg ctattgagaa tttccacaat catcaccagc
    64801 atcacggagc tgataagcag tacgcgggta atcgcgtcca catccagcca gcaggcgata
    64861 accaccgcta acaataccgc tacaccttcc tgacggaatg ccgcttcgtt gatccatgca
    64921 gcgcgtaaac ctttccagga atagccagca gctttgataa ttcgggtgaa tccagtggta
    64981 ttattggcca ttgaaagaac ctttttacat tatgagcgtc aatatcagtg taccgttaaa
    65041 tccgatgatt cgggtttgcg taacgccgcg cgaaacatga gcggatacca cagaatttcc
    65101 catgactttc tgctatcctt gccgcgcatt tgcattatta accagaggct ttacatcgtt
    65161 tatgtccggc tggccacgaa tttactacaa attactgaat ttaccattaa gcatcctggt
    65221 aaaaagcaag tctattccgg cggatcctgc cccggaactg gggctggata cctctcgtcc
    65281 aattatgtac gttttgccgt acaactcgaa agctgacttg ctgacgttgc gcgcccagtg
    65341 tctggcacat gacctgcctg acccgttaga gccgctggaa atcgacggca cgctactgcc
    65401 gcgctatgtg ttcattcacg gcgggccgcg tgtgttcacc tattacacgc cgaaagaaga
    65461 gtctattaag ctgttccacg actatctcga tttgcaccgc agcaacccaa atctggatgt
    65521 gcagatggtg ccagtatcgg tgatgtttgg tcgcgcgccg gggcgtgaaa aaggcgaagt
    65581 gaacccgccg ctgcgtatgc ttaacggcgt acagaaattt ttcgctgtac tgtggctcgg
    65641 tcgcgacagt tttgtgcgtt tctcgccgtc agtttcgctg cgccgtatgg cggatgaaca
    65701 cggcacggat aaaactatcg ctcagaaact ggcgcgcgta gcacgtatgc actttgcccg
    65761 tcaacgcctg gctgccgtgg gtccacgtct gcctgctcgt caggatctgt ttaataagct
    65821 gctcgcctcc cgcgccattg cgaaagcggt agaagatgaa gcgcgcagca aaaaaatctc
    65881 ccatgaaaaa gcgcagcaga acgcgattgc gctgatggaa gagattgcgg cgaatttctc
    65941 ttacgagatg atccgcctga ctgaccgcat tctgggcttc acctggaacc gactttacca
    66001 gggtatcaac gtccataacg ccgagcgcgt tcgccagctg gcccacgacg gccatgagct
    66061 agtatatgtg ccttgccacc gcagtcacat ggactacctg ctgctttctt acgtgctgta
    66121 tcaccagggg ctggtgccgc cgcatatcgc cgccgggatc aacctgaact tctggccggc
    66181 cgggccgatt ttccgccgtc tgggggcgtt ctttattcgc cgtacgttta aaggcaataa
    66241 actttattcc accgttttcc gtgagtatct cggcgaactg ttcagccgtg gttattccgt
    66301 cgagtatttc gtggaaggcg gtcgttcccg tacggggcgt ttgctggatc cgaaaaccgg
    66361 tacgctgtcg atgaccattc aggcgatgct gcgtggcggc actcgtccga ttacgctgat
    66421 tccgatctat atcggttatg agcacgtcat ggaagtgggt acttacgcca aagaactgcg
    66481 cggtgcgacg aaagagaaag agagcctgcc gcagatgctg cgtggtttaa gcaagctgcg
    66541 taatctcggt cagggttacg tcaacttcgg tgaaccaatg ccgttgatga cctaccttaa
    66601 ccagcacgta ccagactggc gtgaatctat cgatcccatc gaagcggtgc gtccggcctg
    66661 gttaacgccg acggtcaata atattgctgc cgatctgatg gtacgcatta acaacgcagg
    66721 cgcggcaaac gccatgaacc tgtgctgtac tgcgcttctg gcgtcacgtc agcgctcact
    66781 cacccgcgag cagttaaccg agcaactcaa ctgctacctg gatctgatgc gcaacgtacc
    66841 ttactccacg gactctaccg ttccttcagc cagcgccagc gagcttatcg atcacgcgct
    66901 gcaaatgaac aagtttgaag ttgagaaaga cactatcggc gacatcatca ttctgccgcg
    66961 cgagcaagcg gtgctgatga cctactatcg caacaacatt gcacatatgt tggtgctgcc
    67021 ttcgctgatg gcggcaattg tcacccagca tcgccacatc tcccgcgacg tattgatgga
    67081 gcacgtcaat gtgctttacc caatgctgaa agcggagctg ttcctgcgct gggatcgcga
    67141 cgagttaccg gacgttattg atgcgctggc aaatgagatg caacgtcagg ggctgattac
    67201 cctgcaagat gatgagttgc atatcaaccc ggcgcattct cgcacgctac agctgctggc
    67261 cgcaggcgcg cgcgaaacgc tgcaacgtta tgccatcacc ttctggctgt tgagtgccaa
    67321 cccgtcgatc aaccgcggta cgctggagaa agagagccgc accgtcgcgc aacgtctctc
    67381 cgtgctgcac ggtatcaacg cgccggagtt cttcgacaag gcggtgttca gttctctggt
    67441 gctgacactg cgtgatgaag ggtatatcag cgatagcggc gacgccgaac cggcagaaac
    67501 gatgaaggtg tatcagttgc tggcggagtt gattacatca gacgtgcgtt tgacgattga
    67561 gagtgcgacg cagggcgaag ggtaatcagt gagaattgcc ggatgcgacg caaacgcctt
    67621 attcggccta ccatgatctg caaattcaat aaattgcgat atgttgtgta ggcctgagaa
    67681 gcgcagcgca tcaggcaacc cagaagaaag ccggatgatc atccggcttt tttacatcat
    67741 cagaaatgcc agtaactcat tgccagccct aaaaacagta ccagaccaac atagttatta
    67801 ttcataaatg ctttaaagca ggcttcacgc tcgcggttgg caatcagttt ttgttgataa
    67861 acaaacagcg cgccagctac cagaattgac cagtaatatc cccagcctaa gccatttaac
    67921 tcaccgatga tcgccatcag cgctagtacg ccaatctgca aaataccaat aatcaattta
    67981 tcgtattggc cgaacaggat tgcggtggat ttaatgccaa tcttcacatc atcatcgcgg
    68041 tcaaccatcg catactgcgt gtcgtaagcc accgcccaga gaatattggc gaggaacatt
    68101 aaccagcaac tcaatggcac cgactcactc acagcggcaa aagccattgg aatcgaccag
    68161 ccaaacgccg cgcccagcac cacttgcggt agatgggtat accgcttcat aaacgggtat
    68221 acccacgcca gcgccagcgc ggcaatcgac aacagaatag tcatcgtatt cagcgtcagc
    68281 accagtaaaa atgaaatcag taccagcact acaaacagcg cgcgcgcctc tttctccgtt
    68341 accgcgccgc tgggaagtgg tcggttcgcc gtgcgcttaa catgaccatc aaacttgcgg
    68401 tcagcataat cattcaccac gcaaccagca gcgcgcatca gccagacgcc cgcgacaaac
    68461 accgccagga tccagagctg gggaacgccc ggtgtcgcca cccacaacgc ccataatgtt
    68521 ggccagagca gcagtaacgc gccaattggc ttatccgtac gcattaagcg atgaaacgcc
    68581 agcagcttat tctgcgtcag actccactcc atattttttt cctcttagta caacggtgac
    68641 gccggtaaaa acagttctgt tagcaacagc ggtttaccgc ttaatcgcag gcgggaacgt
    68701 cgcccccaca gtccggcatc acggcctatc tcaataaagt cccgggttaa tgtcgatgat
    68761 gtgaacagat agcgtcctaa cggcgtttta cctaattttt gtaacgccag ctccggcccg
    68821 cttaacgttg acacaggaac gacggtacga ccggcaagcc acggttcacc atcggcacat
    68881 aacaaaattt cacgtaacca gtaacgagac tctttcggca gcagtggcag ttcttcgggg
    68941 atttcattct gctcgacaaa cccttcgcgg atcatcgtca cgctcaccgt ttttccctgc
    69001 tgttcaaaac gttttgtcat ggaatcttcc agcaacagcc agtcgagcag ttgcggctcc
    69061 agggcaggga tctctgtaaa atagcgcagc gcacgcagtt gcgttaacgc ggggtgtgac
    69121 atgccgaact ctccgttaca taacgtaaag gcattgtatc gcagaaaaat atttacggga
    69181 gatgtaaagc aaattaagtg aactaatgcg caacaaaaac gcaacaatag tacaggagga
    69241 gaaaaaaagg tgcgccagga gacgcaccag ttgttgcaaa atcagcactg tggggcgtaa
    69301 ttaccccttg ccttttacac tgctgataaa ggtggaacgg gcagatgtcg accccaggcg
    69361 ttcagcttca tcaagcagtt ttaacgcctt atcaacatca cctttcgcga cagcgttttt
    69421 gatcgcggta ttaaaataac tttccgtgtc gttgagcatc ggctcgcttt tcttcaccgg
    69481 tgccggagcg ggtgcagcca cagctggtgc cgccgtgttg cctaccgtaa ccggagctgg
    69541 agcggaagaa ccaaatagtg gtcctaccaa cacgctggag ctggagttcg ttttcacttt
    69601 cagtttcagt aagccatcgg tggtatgacg agcaaccgga tcgggaatat ccgggatcga
    69661 gttaccgacg cctttggcat aggctttagc cgggtcgagc agttgagtcg tctgctggag
    69721 atctttttcc gtggtaaaga ccagaacata aagtttttgc tgccccaacg ccggtgtcag
    69781 gcgcataacg ccttccagcc gatctgcact catcacgccg ggttcctggt aggtgaaata
    69841 actgctgggg aagaaggctg atggggtcat gttctgatca agaatcagca cattcggcgc
    69901 aaatacgctg gtttgtttgt tcacttcgct ggtcagcgtc agtgtcagtt caccaatgtt
    69961 tgccgggacg ctgtacgcag caaccggacc actgatgcca gggacattca gttgttggcc
    70021 gccggtcgac agttgagtag tctgggtttt agattgatcg accggtgtcc aggtgagttg
    70081 ttgcagcgcg gcagatggaa tggctggcgc gtcgctggtg ttttgcggta cgtagttaac
    70141 atcggcaaga ctgattccag gtgcgcttgc cagcaaccct gctgataaac agagagcgat
    70201 gagactttta ttcattttca ttgttttcac ctcaaaatct ggagctcagc ggtagccagg
    70261 caatagcgcg ctaaacccga taatcagagg ggctttcgcc ccttaaagct gtttttatgc
    70321 cggatgcggc gtaaacgcct tatccggcct actttcggca gcagttgtag gcctgataag
    70381 acgcaacagc gtcgcatcag gcgttggttg ccggatgcgg cgtaaacgcc ttatccggcc
    70441 tactttcggc agcagttgta ggcctgataa gacgcaacag cgtcgcatca ggcattggtt
    70501 gccggatgcg gcgtaaacgc cttatccggc ctactttcgg cagcggttgt aggcctgata
    70561 agacgcaaca gcgtcgcatc aggcattggt tgccggatgc ggcgcaagca ccttatcagg
    70621 cccaggtttt tctattacca ccagatttcc atctgggcac cgaaggtcca ctcgtcgctg
    70681 tcgccacgac cgaagctacc gccgttgaaa tcagccgaca cagccttgcc gtagtatggg
    70741 ttggttgaag agctaccggt gtagtcataa ccccatttct catcccactt ggcgtaagtt
    70801 gcgaagacac gaatagccgg gcgtgaccag atgctgtcgc cagcctgcca ttgttgtgcg
    70861 agggtaattt tgtactgatt gttcttgtcg ccggtgcgct gggattcgac gttgtcgtag
    70921 ccgatttcca tcacggtgct catgattggc gtccacttgt acatcgggcg aataccgacg
    70981 gtccaccact tggtgccgtt gtcgttatcc cagttgatat cctggtacat acccacgtac
    71041 atcatgtccc agttgtcgcc catggagatc gcaccgtggt cgaggatacg cagcatgtga
    71101 ccgttgttat tgatattgta ggcaaatttt tcgttatcaa acgcgacgcc agaaccctgc
    71161 gacagacctt taccctgcga ggtcatcgag tcagtagcgt actgaacaac aaacttgtta
    71221 aaacccttca ggacactctg agtatgttca gcagtgaata accagccgtc tttcgatgcg
    71281 ccatcaacca gacgatagtt atcacgcagg ttggcacgac cgtagtcgac acccagttct
    71341 aatgtgccgc ccgggttgat ttccatctgc gctaaacgca catcgaaaac gtcgttcgcg
    71401 gtttcgttgg tatagtcata aatattgttg ctggcgaaag aggaagaacc acccgcttca
    71461 gaggagcggg ttgctgccag agagagttta ccgaagccaa catcgatgtt ttccagaccg
    71521 gcaccaggac cagaaatatc ccagtagtag aagtcgatca tatgaacgtc atgacgttgg
    71581 tagaagcgct tacctgccca gatggtggag cctggcagcc attcgatcag gtttttaccc
    71641 tgcacgtttg cttcacggaa ggccggatcg gtagcttccc agtcattctg ttgcgcgacg
    71701 gaataggcca cgttagtgtc gaaatagaag ctcttatcgc cctctttcca cacttcctga
    71761 cccaatttta attcagcata agtttcacat tcgttgccaa gacggtattt actttgagca
    71821 ccggtagtct ggaaacactg ttgttcgccg ccgctacctg tccagccaat accggaacgt
    71881 gcatagccgt ggaaatcaac agccattgcc tgagcagaca ttacgcccgc tgcgacggca
    71941 accgccagag gaagtttgcg cagagtaatc atcattctat ctcctgagtc attgcttttc
    72001 ttttttcaca tcacctgtga caggctttgt gtgttttgtg gggtgcttaa acgcccggct
    72061 ccttatgcag tcgacgacat gcagtgccat cctcacggaa cagatggcaa cgctctggcg
    72121 gcaggccgat agcgaatgtg gcaccttctt ctaccaacac cacgtcgttc tggcggtaca
    72181 ccaggttttg acgaatggaa gggatctgga tatggatttg cgtttcgttg ccgagttgct
    72241 cgacgacctg aacttcaccc tcaaggatga cgtcagcgat atcactcggc agtagatgtt
    72301 ccgggcgaat acccagtgac atattggctc caacctggac atcacggctt tcgaccggta
    72361 gccagacttg ctgacgattc ggcatcggca gctccacctg cacttgatcg attgcggtgg
    72421 cggtcacttt tactggcagg aagttcatct ttggagaacc gataaatccg gcgacaaaac
    72481 ggtctgccgg atagtgatac agctccagcg gtttcccaac ctgcgccacg cgaccagcgt
    72541 ccagcaccac gattttgtcg gccagcgtca tcgcttcgac ctgatcgtgg gtgacgtaaa
    72601 tcattgtgcg gcccaggcgt ttatgcagac gggagatttc gatacgcatt tgcacacgca
    72661 gtgcagcatc gaggttggag agcggttcat cgagcaaaaa tacgcttggc tcggccacca
    72721 gcgtacggcc aatcgccaca cgctgacgct gaccaccgga gagcgctttc ggtttgcgat
    72781 ccagcaaatg cgccagttgc agcacttccg caacctggtt aactcgctgg ttaatcactt
    72841 cttttttcgc gccagccagt ttcaggccaa atgacatgtt ttctgctact gacaggtggg
    72901 gatagagcgc gtaagactga aacaccatac caacgccgcg ttctgcaggc ggagtgtcat
    72961 tcatccgttt ctcaccgatg aacaggtcgc cgctggtgat catctcaagc ccggcaatca
    73021 tgcgcagtaa agtcgattta ccgcagccag acggtccgac aaacaccacg aattcacctt
    73081 catggatatc gagattgata tctttcgata ccacgacctc gccccaggct ttcgttacat
    73141 tttgcagctg tacgctcgcc atgcccttct ccctttgtaa caacctgtca tcgacagcaa
    73201 cattcatgat gggctgacta tgcgtcatca ggagatggct taaatcctcc accccctggc
    73261 ttttttatgg gggaggaggc gggaggatga gaacgcggct tctgtgaact aaaccgaggt
    73321 catgtaagga atttcgtgat gttgcttgca aaaatcgtgg cgattttatg tgcgcatctc
    73381 cacattaccg ccaattctgt aacagagatc acacaaagca acgatggggc gtaggggcca
    73441 ggaggatgga aagaggttgc cgtataaaga aactagagtc cgtttaggtg ttttcacgag
    73501 cacttcacca acaaggacca tagattatga aaataaaaac aggtgcacgc atcctcgcat
    73561 tatccgcatt aacgacgatg atgttttccg cctcggctct cgccaaaatc gaagaaggta
    73621 aactggtaat ctggattaac ggcgataaag gctataacgg tctcgctgaa gtcggtaaga
    73681 aattcgagaa agataccgga attaaagtca ccgttgagca tccggataaa ctggaagaga
    73741 aattccctca ggttgcggca actggcgatg gccctgacat tatcttctgg gcgcacgacc
    73801 gctttggtgg ctacgctcaa tctggcctgt tggctgaaat caccccggac aaagcgttcc
    73861 aggacaagct gtatccgttt acctgggatg ccgtacgtta caacggcaag ctgattgctt
    73921 acccgatcgc tgtcgaagcg ttatcactga tttataacaa agatctgctg ccgaacccgc
    73981 caaaaacctg ggaagagatc ccggcgctgg ataaagaact gaaagcgaaa ggtaagagcg
    74041 cgctgatgtt caacctgcaa gaaccatact tcacctggcc gctaattgcg gctgacgggg
    74101 gttatgcgtt caagtatgaa aatggcaagt acgacattaa agacgtaggc gtggataacg
    74161 ctggcgcgaa agcgggtcta accttcctgg ttgatctgat taaaaacaaa cacatgaatg
    74221 cagacaccga ttactccatc gcagaagctg cctttaataa aggcgaaaca gcgatgacta
    74281 tcaacggccc gtgggcatgg tccaacatcg acaccagtaa agtgaattat ggtgtaacgg
    74341 tactgccgac cttcaaaggt caaccatcta aaccgttcgt tggcgtgctg agcgcaggta
    74401 ttaacgccgc cagtccgaac aaagagctgg cgaaagagtt cctcgaaaac tatctgctga
    74461 ctgacgaagg tctggaagcg gtcaataaag acaaaccgct gggtgcagta gcgctgaagt
    74521 cctatcagga tgaacttgcg aaagatccac gtattgccgc caccatggat aacgctcaga
    74581 aaggtgaaat tatgccgaac atcccgcaga tgtccgcttt ctggtatgcc gtgcgtactg
    74641 cggtgatcaa cgccgccagc ggtcgtcaaa ctgtggatgc tgcgctgaaa gatgcacaga
    74701 ctcgtatcac caagtaatgc tgtgaaatgc cggatgcggc gtaaacgcct tatccggcct
    74761 acgaaattga aacgtatgta ggcctgataa gacgcgtcag cgtcgcatca ggcagttgtt
    74821 gtcggataag gtatgaaagc cttatccgtc ctggaatgag gaagaacccc atggatgtca
    74881 ttaaaaagaa acattggtgg caaagcgacg cgctgaaatg gtcagtgtta ggtctgctcg
    74941 gcctgctggt gggttacctt gttgttttaa tgtacgcaca aggggaatac ctgttcgcca
    75001 ttaccacgct gatattgagc tcagcggggc tgtatatttt cgccaatcgt aaagcctacg
    75061 cctggcgcta tgtttacccg ggaatggctg gaatgggatt attcgtcctc ttccctctgg
    75121 tctgcaccat cgccattgcc ttcaccaact acagcagcac taaccagctg acttttgaac
    75181 gtgcgcagga agtgttgcta gatcgctcct ggcaagcagg caaaatctat aattttggtc
    75241 tttatccggc gggcgatgag tggcaactgg cgctcagcga cggcgaaacc ggcaaaaatt
    75301 acctctccga cgcttttaaa tttggcggcg agcaaaaact gcaactgaaa gaaagcgcca
    75361 cgcagcccga aggcgaacgc gcgaatctgc gcgtaattac ccagaatcgt caggcactga
    75421 gtgacattac cgccattctg ccggatggca acaaagtgat gatgagctcc ctgcgccagt
    75481 tttctggcac gcagccgctc tacacactcg acggtgacgg cacgttgacg aataaccaga
    75541 gcggcgtgaa atatcggccg aataaccaaa ttggttttta ccaatccatt accgccgacg
    75601 gtaactgggg ggatgaaaag ctaagccccg gttacaccgt gaccaccggc tggaaaaact
    75661 ttacccgcgt ctttaccgac gaaggcattc agaaaccgtt cctcgccatt ttcgtctgga
    75721 ccgtggtgtt ctcgctgatc actgtctttt taacggtggc ggtcggcatg gttctggcgt
    75781 gtctggtgca gtgggaagcg ttgcgcggca aagcggtcta tcgcgtcctg ctgattctgc
    75841 cctacgcggt gccatcgttc atttcaatct tgattttcaa agggttgttt aaccagagtt
    75901 tcggtgaaat caacatgatg ttgagcgcgc tgtttggcgt gaagcccgcc tggttcagcg
    75961 atccgaccac cgcccgcacg atgctgatta tcgtcaacac ctggctgggt tatccgtaca
    76021 tgatgatcct ctgcatgggc ttgctgaaag cgattccgga cgatttgtat gaagcctcag
    76081 caatggatgg cgcaggtcca ttccagaact tctttaagat tacgctgccg ctgctgatta
    76141 aaccgctgac gccgctgatg atcgccagct ttgcctttaa ctttaacaac ttcgtgctga
    76201 ttcaactgtt aaccaacggc ggcccggatc gtcttggcac gaccacgcca gccggttata
    76261 ccgacctgct tgttaactac acctaccgta tcgcttttga aggcggcggg ggtcaggact
    76321 tcggtctggc agcagcaatt gccacgctga tcttcctgct ggtgggcgcg ctggcgatag
    76381 tgaacctgaa agccacgcga atgaagtttg attaagggag ataacaaaaa tggcaatggt
    76441 ccaaccgaaa tcgcaaaaag cacgtttgtt tattactcac ctgctattgc tactttttat
    76501 cgcggcaatt atgttcccgc tgctgatggt cgtcgccatc tcgctgcgtc aggggaactt
    76561 cgcgaccggt agtctgatcc cggagcaaat ctcctgggat cactggaaac tggcgttagg
    76621 ttttagcgtg gaacaggctg atggtcgcat tacgccaccg ccattcccgg tactgctgtg
    76681 gctgtggaac tcggtaaagg tcgccgggat ttccgcgatt ggcattgtgg cgctctccac
    76741 cacctgcgcc tacgctttcg cccgtatgcg ctttccaggc aaagcgacgc tgctgaaagg
    76801 aatgctgatt ttccagatgt tcccggcagt actttcactg gtcgcgttgt atgcgttgtt
    76861 tgatcgtctg ggtgagtaca ttccattcat tggcctgaat actcacggcg gcgtaatttt
    76921 cgcgtatctg ggtgggattg cgctgcatgt ctggaccatc aaaggttatt tcgaaaccat
    76981 cgacagttcg ctggaagaag cggcggcgct ggatggtgcg acaccgtggc aggccttccg
    77041 ccttgtcctg ttgccgctgt cagtaccgat tctggcggtt gtgttcatcc tgtcgtttat
    77101 cgctgccatt actgaagttc cggtcgcgtc gctattactg cgtgacgtaa acagctacac
    77161 cctggccgtg gggatgcagc aatacctcaa cccgcaaaac tacctgtggg gtgactttgc
    77221 cgccgctgcc gtgatgtctg cattaccgat caccatcgtc ttcttgctgg ctcaacgctg
    77281 gctggtcaac ggcctgacgg caggtggtgt gaaaggttaa agatgttgtt ctgccaatgt
    77341 tatgccgctg caccctcaac ttacgttatc ccaacttgtg actgttattc ggcgctccac
    77401 ggagcgcctt tttttactcc cgcttcagcc gtttcgaatt acacaaccac agggtgatca
    77461 ccagtaacag gatcgctgcc gagtagatca acacatccag tggcgattta tgatcgacga
    77521 tgatcaagcg cacaatggcg gtgatcccaa tatagacaaa gtaacgtagc gggaagtgaa
    77581 aaccggactg aaagtacttc acaatcagcg cgataaattc gaaatagaga aagtaaacca
    77641 ccagcccttc taccagctca tatttactgg tttgttctgg cacgaacagc acatcagcca
    77701 gatgcaccgt ttctttgccg aggaagacaa ccaaaatcag gccgaggcac aacaggccaa
    77761 gattgagtac ggtctgcaaa atggtggata taaactccac gcgcggacga gagagtgacg
    77821 tcatagcagt acctccttca cagtggcgag cttcctgtat aacgcaaaaa tgtgacggag
    77881 atctatattt tttgtgtatg ttttgttcag ctcaattaag tgggcatctc ccatcaacgt
    77941 cgcatctggc aaaacacaca gccaaaaaaa tcacccattc aggtgattcc ctgccaccag
    78001 caatgcgcga aaaatcgaca atgttcctct ggaacaaatg aaacgtaacc tgataaaggt
    78061 ggtaaaaatg aagccttatt ttgctgcttt gatgttatca gtctctgttc ttcctgctta
    78121 tgctggtctg ctcggtactg ctgataaagc cgacctaccg caaagcaatg tctccagccc
    78181 aatgatggcc cagtcgctca ggcaaccaga cctgcaaccg atatcgacgg acaggaagac
    78241 agaatgtttt cgcctttaca caccagatcg caaaccgggg gtgaactgcg ttcctgatgg
    78301 ctcgacaggc tattaagaat gaaggtgcca gtgagtgcga aaactggcac ctttcccatg
    78361 acttagcgga aattgacgct acggtcgctg gtcatatcat acaactggaa cttacgccca
    78421 agttgctgac caccgtcacg cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc
    78481 gggccagacg agaagagatc caacggtaca gagacataca cgcctttggt gaagtcccct
    78541 tcgccgtact cctctttcga aacgttggtg atcgtggcat agccgccaac caccacgccg
    78601 ctgtcaaagc gtttagcgat ctccagcgtg ccgcctttat ccccagccag atactgcccg
    78661 acgctcgctt tcactaacac gtcctgggcg aaagatggcg tccagtacgc ggtcagatgt
    78721 ccggttttca cgctgtaatc ggtgaatttc atcatatctt ttgcgctacg ccagtcgcgc
    78781 tgtttaacgt agttggcatc cagaccaaac gcccagttgc tatccagcgg gcgatacaac
    78841 acttctgccc ccacgccgcc aaacatggtt tcgagataac ctccgtagac ctgaccgtag
    78901 aagccgttgc ccagatgctg gaagtagttg gcttgcaggt tattcacata gacatcattc
    78961 tgcacatact cgcgcacatg ggtacgcacg cgcggtaagt gcgagtcctg cggtggattg
    79021 gtgtagttaa acttgtcgta gttgttggca aggttagcaa acaggctgcc ggtggtcagc
    79081 agatggtccg tcagccacaa atccgccgtt cccatcacgc ccagctgata catgtaaaag
    79141 ttttccgggc caccgaccga ctggttcagc accggatcga tatggaaatc aaagcgtgat
    79201 ttgtcgatat accagccctg ctcggtggac ttcggaacca ctggctcgac gcgtttttgc
    79261 gccagcgtcg tttcgtggcc caacggctct ccgccgagat gatttttcag gctggcgaca
    79321 tcggtttccg tcgtcacctg cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg
    79381 atcccatccg gcagatcgtt catcacgatc cgattagcac ggatgatccc ttcgcgcgaa
    79441 tcacgatatt tcacctgctc gccggtaaca tacagcgtat cgcctttcgc ctggatctgc
    79501 ggatcggcca gtccggcgtt gtatttcaac agcgttaact gattcgccac caccgagtgc
    79561 tgcaaaatgg catcctgcgg ttgcggctgg tatttcggtc gggcgttatc gttgtaggac
    79621 gggcgcagat cgttaaagtt ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt
    79681 tcatagctaa ggttaacgtc ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac
    79741 ttactttttt gctccagctt cccggcaaaa tcctgctgat aattattacc ttcatactcc
    79801 agtttcaggc gcagcggttg ccagggcgtc tggtattcca cgccgccaaa cagtgaggcc
    79861 gggccgtgga acatctgact gccgtcgata gatcccgctt gtttgtagct gttatcgcga
    79921 tagcaatatt tatcactggc tgaacagagc ggatttttca cattaccgct ggtgcccaga
    79981 tatccccatc ccaggccgag cgtaaaatcg aacggccccc aggctttgct ggcaacaaga
    80041 tattccgcat caaacagccc cgtaccgccg atatcccgcg caccaaccgc cacttgcggc
    80101 agccaataac tctcttccca caaacgcagt ttgagatcga aggctttatc tttatacgtt
    80161 tgatcgccag agaacgcttc gacgctgctg tactgccggg tgcgcacgtc ggtgtagcgc
    80221 agcgttgttt ccagccacgg gaagagttgc actgaagctg agtaataacg gtactgatcg
    80281 ttatcgcgat agttcaggct cagctcccct tcccgcgcca tgcgcgcggt gggcgtttgt
    80341 aataatccta cgccaccgaa atccgattgc gacggaccaa tgggcgcagg atatgtttcg
    80401 ccgtagcaag ctgtgctaat gcccagcgcc agtaagctaa gcagatgtct ttttttcatt
    80461 attggggtat ccgctgcgtc agagtctgaa gaatgtcggc gttaagggca tcaggtgtct
    80521 cactccagac ggagtccgcg aggccaacat aaataatgct gccgggcatc ggctctacgt
    80581 gacgcttgtt ccaataagcc accggcgctt tttgcgtgcg tccgtccggg taaaccaccc
    80641 acgcgtagct gcgatccgca ccgctgagca ggctctggtc agagagatag ctcgccacgt
    80701 cgcgacctag agtgaatgac tgcttgccag gacgactgat aagcccgaac aacgtgaccg
    80761 tggacggcgg cgttccgacc cacagcgtgt agttgccctg caacggcggg ttaccgcgtt
    80821 cggcaacgcg gacgatatca ggatcaagat tgattttttg cctgcccgtc accttcaacg
    80881 cctgaatttg ctggcgtaag gcgttaatcg cggcggcatc gtcggcgctg gaatctgcag
    80941 ccagttctgc cagccgtgtc agcaacgcct gctgctgacg caatgcagcc gccgtggcca
    81001 gctcttcgct aatcaccgct ccgggccacc agctgtttgc cagccgcggt tggcctacca
    81061 gatcgattaa atgctctgcg cccgtcagcg ttttggcctc actgctgcca ttgctgaaca
    81121 ccttgacggt tcccgccgcg aagactgatg acgcccccgc actcaaaagc aacgcgacaa
    81181 tagtttgttt aatcattgtt ttgccgcctt gatgagggtg gttttcaccg ggaagtaatc
    81241 cgcgccgaga tactgttccg actggcgaat ttgcccttcg cgtcgatcca atagcggtta
    81301 tgccagcgcg cctggtcagt gaagacttct tcgtccagca cgcgaaccgg ggtttcatcg
    81361 ctgccgactt tgacggtatc ggtgccattc catttgaaga ctgagcgcgc ggtggcgtag
    81421 cgtacctgct ggtactcggt ccagcccatc gtgcgcgtcc aggttgcacc atcaatgatt
    81481 tgtgcgggtt taatcagcgg gtcggcggca aggttattca cttcaatcag gttgtcgccg
    81541 ccaagcaaag tcttcaccag acgaccatgt tgtgtgacaa gggtggcctg atcctgagtg
    81601 acccattttt gttgtccgtc ttcagcgaag gccagcacca caaataactg cggcccgcca
    81661 ttaagctgca tgtactggct ggcgtagggc atattttgaa tatcatcgtc cgtcagctgt
    81721 acgcctggcg taccgaacag actgtcccat agtgaattgc ccagctcttt agtcgtggct
    81781 gaacaggcct gtaatagcag gcagataaga atgagtgcag gtcgcttcac gactcttctc
    81841 cagaggggca gaataaccac accggagtgt ggttatggtt aatccccttc atacttcaag
    81901 ctgcagatgc ggtgaccgca ctcgttcacc ccggtcacat agttatctat gctcccgggg
    81961 actcactcgc ttgtcgcctt cctgcaactc gaattatttt ggggatacat actttattac
    82021 tgggtactgg tggtcgtggt tgtggtggtc ccggtattag aaccatcacc gccaccggtt
    82081 gccgcgagcg cgacacccac cgccgagctt acggtgctga cgctggtcgc ggaggaacta
    82141 cccgccgaaa ccgacgttgc tgccgatcct gccgcttcgc ccacctgtac aggtgcagcc
    82201 cacgcagaag tcgccgcaag cgcagatatg gcaaaaatgc catacagaac ttttttcata
    82261 acaatttccc ttcattgaat gaatggagat ttacccaaaa catttcgggc gggatcgagt
    82321 atacacaact aaagcatgcg aattacggcg cggggaatta gtgatggagt tttaggacaa
    82381 ttggaatgaa tatcaaatat aagctttgac ttatatataa ataagattaa aaatcatgaa
    82441 gttaaaataa aataaaaagt aattactctg cgtattttcc gaaataaacc acgattaaaa
    82501 acttatttta ggattaacct cacggtatga ttttcgttaa atgacagaca agaggattta
    82561 tctgataaaa caatgccgac ttatagtcgg cattgttttt aaggctattt aatcaatcgt
    82621 actatgtatt aattgccgaa tacgatgtac atatcggcat cgacctgtag gcctgataag
    82681 acgcgaccgc gtcgcatcag gcatcggttg ccggatgcgg cgtgaacgcc ttatccggcc
    82741 tacatatcga caatgattaa ccgcgccacg ctttatagcg gttaatcaga ccattggtcg
    82801 agctatcgtg gctgctgatt tctttgtcat ctttcagctc tggcagaata cggttcgcca
    82861 gctgtttacc cagttccacg ccccactggt cgaaggtgaa gatgttcagg atcacgccct
    82921 gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc caggctgaac ggagtgattt
    82981 cacgcagcag gatggagtta gtcgggcggt taccttcgaa cactttgaac ggcaccacgt
    83041 agtcaagcgt tgccggatct ttaccctgat cgcgatattc ctgctcaacc acttcgcggg
    83101 atttaccaaa cgccagcgct tcggtctgag caaagaagtt agacagcagt ttctggtgat
    83161 gatcagagag cgggttatgg gtgatagccg gagcgatgaa atcgcacggt accattttgg
    83221 ttccctggtg gatcagctga tagaacgcgt gctgaccgtt cgtgccaggt tcaccccaga
    83281 taatcgggcc agtctggtaa tccacaacct taccgttacg gtcaacatac ttaccgttgg
    83341 attccatatt gccctgctgg aagtacgccg cgaaacggtg catatactgg tcatacggca
    83401 gaatcgcttc agtttccgcg ccaaagaaat tgttgtacca gatgccaatc agcgccagca
    83461 gtacaggcag gtttttctcg gcaggcgtgg tggagaaatg cttgtccatc gcgtgtgcgc
    83521 cagaaagcag ttcaacgaag ttatcaaagc cgatggagag aacaatcgac aggccaatcg
    83581 ctgaccacaa agagtaacga ccgccgaccc agtcccagaa ctcgaacatg ttggcagtat
    83641 caataccaaa ctcgccaacg gctttggcgt tggttgagag cgccgcaaag tgtttcgcaa
    83701 cgtgcttctc atcacctgcc gctttcagga accagtcacg cgcgctgtgg gcgttggtca
    83761 tggtttcctg agtggtgaag gttttagatg ctaccaggaa cagcgtggtt tccgggttta
    83821 cttttttcag cacttccgcg atgtgagttc catcgacgtt agaaacaaag tgcatgttca
    83881 ggtggttttt gtatggacgc agtgcttcag tcaccatgta cgggccgagg tcagaaccgc
    83941 cgatcccgat gttcactacg tcagtgattg ccttgccggt atagcctttc cactcaccgg
    84001 aaataatcgc ttccgagaag gttttcatct tctccagcac cgcgttgact tccggcataa
    84061 catctttgcc atcaaccaga atcggggtat tgctacggtt acgcagcgct acgtgcagta
    84121 cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa catcgactta atcgcgcccg
    84181 ccagatcgca ctctttcgcc agatcttgta atttcgccag cgtctcttca gtgatgcggt
    84241 ttttggagta atccaccagc atctgatcgt cgaaggttgc ggagaactta gaaaaacgat
    84301 cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt catttcatcg aagtgtttct
    84361 gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt tttcattagc aatactcttc
    84421 tgattttgag aattgtgact ttggaagatt gtagcgccag tcacagaaaa atgtgatggt
    84481 tttagtgccg ttagcgtaat gttgagtgta aacccttagc gcagcgaagc atttattagc
    84541 tgaactactg accgccagga gtggatgaaa aatccgcatg accccatcgt tgacaaccgc
    84601 cccgctcacc ctttatttat aaatgtacta cctgcgctag cgcaggccag aagaggcgcg
    84661 ttgcccaagt aacggtgttg gaggagccag tcctgtgata acacctgagg gggtgcatcg
    84721 ccgaggtgat tgaacggctg gctacgttca tcatcggcta caggggctga atcccctggg
    84781 ttgtcaccag aagcgctcgc agtcgggcgt ttcgcaagtg gtggagcact tctgggtgaa
    84841 aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg ctcttccctt gtgccaaggc
    84901 tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa ttgttgtctc caaatttggc
    84961 ggtaccagcg tagctgattt tgacgccatg aaccgcagcg ctgatattgt gctttctgat
    85021 gccaacgtac gtttagttgt cctctcggct tctgctggta tcactaatct actggtcgct
    85081 ttagctgaag gactggaacc tggcgagcga ttcgaaaaac tcgacgcaat ccgcaatatc
    85141 cagtttgcca ttctggaacg tctgcgttac ccgaacgtta tccgtgaaga gattgaacgt
    85201 ctgctggaga atattactgt tctggcagaa gcggcggcgc tggcaacgtc tccggccctg
    85261 acagatgaac tggtcagcca cggcgagctg atgtcgaccc tgctgtttgt cgagatcctg
    85321 cgcgaacgcg atgttcaggc acagtggttt gatgtacgta aagtgatgcg taccaacgat
    85381 cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg aactggccgc gctgcagctg
    85441 cttccacgcc tcaatgaagg cttagtgatc acccagggat ttatcggcag cgaaaataaa
    85501 ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata cggcagcctt gctggcggag
    85561 gctttacacg catctcgtgt tgatatctgg accgacgtcc cgggcatcta caccaccgat
    85621 ccacgcgtgg tttccgcagc aaaacgcatt gatgaaatcg cgtttgccga agcggcagag
    85681 atggcaactt ttggtgcaaa agtactgcat ccggcaacgt tgctacccgc agtacgcagc
    85741 gatatcccag tctttgtcgg ctccagcaaa gacccacgcg caggtggtac gctggtgtgc
    85801 aataaaactg aaaatccgcc gctgttccgc gcgctggcgc ttcgtcgcaa tcagactctg
    85861 ctcactttgc acagcctgaa tatgctgcat tctcgcggtt tcctcgcgga agttttcggc
    85921 atcctcgcgc ggcataatat ttcggtagac ttaatcacca cgtcagaagt gagtgtggca
    85981 ttaacccttg ataccaccgg ttcaacctcc actggcgata cgttgctgac gcaatccctg
    86041 ctgatggagc tttccgcact gtgccgggtg gaagtggaag agggtctggc gctggtcgcg
    86101 ttgattggca atgacctgtc aaaagcctgc ggcgttggca aagaggtatt cggcgtactg
    86161 gaaccattca acattcgcat gatttgttac ggcgcatcca gccataacct gtgcttcctg
    86221 gtgcccggcg aagatgccga gcaggtagtg cagaagctgc attttaattt gtttgagtaa
    86281 acacagtatg gcccggaaga cattatttcg ggccgtattg tttttcttgt cattatgccc
    86341 ataaataaac gagtctattc cctgttaacc agcgtcttta tcggagaata attgccttta
    86401 attttttatc cgcatctcta actaattatc gaaagagata aatagttaag agaaggcaaa
    86461 atgaatatta tcagttctgc tcgcaaacga attcccgtac actgaactct ctggctcact
    86521 gagggcacca tcatggcact cccccgtatt acccaaaaag agatgactga acgcgagcag
    86581 cgtgaactta aaacactgct ggatcgggcg cggattgcgc atggtcgcgt actgaccaac
    86641 tccgaaacta acagcattaa gaaagagtac atcgataaat taatggtcga gcgtgaggct
    86701 gaagcgaaaa aagcccgcca attgaagaaa aagcaggctt ataaaccgga tcctgaggcg
    86761 tcatttagct ggtcagcaaa tacgtcgacg cgcggcaggc gctgattaac gccccttctt
    86821 tttgcgcccc ggcgaggtaa aacgcttacc gttggacgcc gggcgaccgc ctttttccgc
    86881 cgttttttcc atcttaacga ctggacgttt gatgcctacc gttttcggtt tggccttcgc
    86941 tttaggtttt gcctcggaag aggaattttc aatgagctta aagagatcga ttaactcatc
    87001 gtcggttaaa tcgcgccact cacccagcgg aatgccgctt aagctgacgt tcataatgcg
    87061 cgtacgttcc agctttttca cttcgtaacc aaagtgctcg cacatgcgac gaatctgacg
    87121 gttcagcccc tgcaccaggg taatgcggaa gacaaacggt gcttcttttt tgactttgca
    87181 ctttttagtt actgtcccga ggatcggtac tcccgccccc ataccacgaa taaattcatc
    87241 ggtaatcggt ttatcaaccg tcaccagata ctctttctca tgatcgttac ccgcacggag
    87301 gatcttattc accagatcgc cgtgattggt gaggaaaatc agcccctggg aatctttatc
    87361 caggcggcca atcgggaaca cgcgtttgct gtggttaacg aaatcgacaa tgttatcgcg
    87421 ctcgccatct tcggtggtgc tgacaatacc gacgggcttg ttcagggcga taagtaccaa
    87481 atcttcggat tcccgaggct caatcaactg accgtttact ttcacaacgt cgccgggttt
    87541 cacctgatcg ccaatggtgg ctcgcttgcc attaaggaac acattgcatt gctcgatata
    87601 gcgatccgct tcgcggcgtg agcaaattcc gctttcgctg atgtatttat ttaaacggac
    87661 tgatgagtcg ggcagcataa attctcctgt aaaacgcgga atataccgta cctcttagcg
    87721 gggaaaaaca gggttatgcg gcaacgtcga aattaaatgt gatctgccac gccttttatc
    87781 acaaaagtgt ctccggaagt acgatgcaaa tttgcctggc aaaataagaa taatcaacaa
    87841 attacgaatc gctgcacacc tgtgctgatg actactacgc agagaaacaa atgtgcaaga
    87901 gaaagcgcta tttcccccca tgcaaagagt gaaaaatcgc tttgaaaggc catcatgatg
    87961 gggttatatc agatcaagcg gtgagaacgc gtatttgcac aaatgtgcag aaccaggagg
    88021 cggcaatgga aaacagtgac gatatccgtt tgattgtgaa gattgcccaa ctctattacg
    88081 aacaggatat gacgcaggcg caaattgcgc gcgaactggg tatttaccgc accaccatca
    88141 gccgcttgct taaacgaggc cgcgatcagg gaattgtcac catcgccatc aactatgact
    88201 acaacgaaaa tctctggctg gagcagcaag tgaagcaaaa gtttggcctg aaagacgttg
    88261 tggtggtgtc aggaaatgat gaggatgaag atactcaact ggcgatgatg gggttacacg
    88321 gcgcgcaact gctggatcgc ttgctggaac ctggcgatat tgtcggtttt tcctggggcc
    88381 gcgcggtgag cgcactggtt gaaaacttgc cgcaggcggg gcaatcgcgg cagttaattt
    88441 gcgtgccgat tattggcggc ccgtccggta aactcgaaag ccgctatcac gtaaacacat
    88501 taacctacag cgcggcagcg aagctgaaag gggaatcgca tctcgcggat tttccggctc
    88561 tgctggataa cccattaatt cgtaatggga tcatgcagtc tcagcacttt aaaaccatct
    88621 ctgcctactg ggataatctg gatgtcgccc tggtgggaat tggctcaccg gccattcgcg
    88681 acggcgctaa ctggcatgcg ttttatggtg gtgaagagag tgacgacctg aatgcccgcc
    88741 aggttgctgg cgatattttc tcgcgctttt ttgatattca cggcgcaatg gttgaaacga
    88801 atatgagcga aaaaacactc tctatcgaaa tgaataaatt aaagcaggca cggtattcca
    88861 ttggcattgc catgagcgaa gaaaaataca gcggaattgt tggtgcactg cgtggaaaat
    88921 atattaattg tctggtaacg aatagcagca cagctgaact attactgaaa taaaaatctt
    88981 cccgatgttt accgtaataa aaattacggc gggtccctat tatctaaaat caggagtctg
    89041 ttatgcaaac gtggttaaat ttgcaggata aaatcattat tgtcaccggt ggcgcatccg
    89101 gcattggtct ggcgattgtg gatgaattat tagcacaagg cgcgaatgta cagatggtcg
    89161 atattcactg tggcgatggt cagtatgaaa gccataaagg ttatcagttc tggccgaccg
    89221 atatttccag cgccaaagag ataaatcata cggtagcaga aattatccag cgttttggtc
    89281 gcatcgacgg tctggttaac aacgccgggg tcaatttccc gcgtctgctg gtcgatgaga
    89341 aagcgcctgc cgggcagtat gaactcaacg aagctgcatt cgaaaaaatg gtcaatatca
    89401 accagaaagg cgtttttctg atgtcgcagg cggtggcgcg acagatggtc aaacaacatg
    89461 atggcgtgat tgtgaatgtt tcctcagaaa gtgggctgga aggctcagaa ggccaaagct
    89521 gttacgccgc gaccaaagcc gcgctcaata gcttcacgcg ctcctggtcg aaagaactgg
    89581 gtaagcacgg tatccgtgtg gtcggtatcg cgccggggat tctggaaaaa acaggactgc
    89641 gcacgccgga atatgaagaa gcgctggcgt ggacgcgcaa tatcaccgtc gagcagctgc
    89701 gtgaaggcta taccaaaaac gccattccta ttgggcgcgc cggaagatta gcagaagtgg
    89761 ctgattttgt ttgttatctg ctgtctgaac gcgccagcta tatcaccgga gtaaccacta
    89821 acattgcagg cggcaaaacg cgcggctaag gaggctttat ggtcaatgcc attttttgcg
    89881 cccacggcaa attagcctgc gccatgctgg aatcggttca gatggtgtac ggcgatgcca
    89941 gggtcgaggc cgttgaattt gtgcccggcg agaacgccgg agacattgtg gcaaagctgg
    90001 ataagttagt aagcactcac aatcatgacg agtggctaat cgccgtggat ttacagtgcg
    90061 gcagcccgtg gaatgcggca gcgatgcttg cgatgcgcaa tcctcgtctg cgggtaatca
    90121 gcggcctttc tctgccgctg gcgctcgaac tggttgataa ccaggacagc atgaatattg
    90181 atgaactgtg tgaacacctg acgcaaatcg caaagcaaac ctgcgtcgtc tggagacagg
    90241 tggcaacagc ggaggaggat ttctgatgaa cattacgctc gcccgcattg atgaccgcct
    90301 gatccacggc caggtcacta ccgtctggtc taaagtggct aacgcccaac gcattattat
    90361 ctgcaatgac gaagtttata acgatgaagt tcgccggaca ttattgcgtc aggctgcgcc
    90421 accgggaatg aaggttaacg tggtcaatat tgaaaaagcc gtcgcggttt atcacaaccc
    90481 gcaatatcag gacgaaaccg ttttttatct tttcactcgc ccacaggatg ccttagcaat
    90541 ggttcgccag ggggtaaaaa taggtaccct gaatattggc ggtatggcct ggcgtcctgg
    90601 taagaaacag ttaaccaaag cagtttcatt agatgacgat gatattaacg catttcatga
    90661 gttaaataat cttggcgtca tactggattt acgcgtcgta gcgtcagatc cctcaatcaa
    90721 tattatcgac aaaataaacg aacagttaat cgcgaattaa ataatagcgc ctgtgtacat
    90781 atgctttaac aggcagggat gacgacactt aaaaggtgac aaatcatgga aataagtacc
    90841 ctacaaatca ttgctatatt tcttttttcc tgtattgccg gaatgggcag cgtgctggat
    90901 gaatttcaga ctcatcgtcc gttaattgcc tgtacggtga ttggtttaat tctcggtgat
    90961 ttaaaaaccg gaattatgct cggtggtacg ctggaattga tagctctcgg ctggatgaac
    91021 gtcggcgcgg cgcaatctcc ggattctgca ctcgccagca taatctccgc cattctggtt
    91081 atcgttggtc agcaaagcat cgccaccgga atcgccatcg cgttgcctgt ggctgctgca
    91141 ggccaggtgc tgacggtgtt tgcccgtacc atcaccgtag tgttccagca cgcggcggat
    91201 aaagcagcgg aagaagcacg gtttcgcact ctcgatattc tgcatgtctc cgcgcttggc
    91261 gtgcaggcac tgcgcgttgc tattccggca ctgattgtct cgctgttcgt cagcgctgat
    91321 atggtgagca atatgctgag cgccattccc gaatttgtga cccgtggact gcagattgct
    91381 ggcggtttta tcgtggtggt cggttacgcc atggtgcttc gcatgatggg cgtgaaatat
    91441 ttgatgcctt tctttttcct cggtttcctc gcaggtggct acctcgatct cagtctgctg
    91501 gcgttcggtg gcgtcggcgt gatcatggcc ctgctctaca tccagttaaa tccacagtgg
    91561 cgtaaagctg aaccacatcc ccagaccacc actatcaccg cccttgacca acttgatgat
    91621 taacggagcc atcatggaac agagaaaaat tacacgcagc gatctggtga gcatgtttct
    91681 gcgctccaac ctgcaacagg catcctttaa ctttgaacgt attcacgggc tgggtttttg
    91741 ctacgacatg atccccgcca tcaaacgact ttacccatta aaagaggatc aggttgcggc
    91801 gctcaggcga cacctggtgt tcttcaatac cacgccagcc gtatgtggcc cggtcatcgg
    91861 cgtcactgct gccatggaag aggcgcgggc caacggcgcg gaaattgatg acggtaccat
    91921 taacggcatc aaagtcggtc tgatgggacc attggcagga gttggcgatc cactggtctg
    91981 gggaacgctg cgcccgatta ccgccgcgct cggcgcatct ctggcacttt cgggcaacat
    92041 tctcggcccc ctgctgttct tctttatttt caacgcggtg cgtctggcga tgaagtggta
    92101 tggcctacag ctcggctttc gcaaaggggt gaatatcgtc agcgatatgg gcggtaattt
    92161 gctgcaaaaa ctcaccgaag gcgcgtcgat tctcgggctg tttgtgatgg gcgtgctggt
    92221 gaccaaatgg acgtcaatca acgtaccgtt ggtggtttca caaacgcctg ccgccgatgg
    92281 tgccaccgtc accataaccg tgcagaacat cctcgaccaa ctttgccctg gtttgctggc
    92341 gctcggtctg acgctgctga tggttcgtct gctcaacaaa aaaattaacc cggtatggct
    92401 gattttcgcc ctgtttggtt tagggattat cggtaatgcg ctgggcttcc tgtcctgatt
    92461 cttcgccccg gcacgactgc cggggtcatc gctcaacatg aggtggttta tgaaaacaac
    92521 agctctgcgt ctttatggca aacgtgacct gcgtctggaa acctttgacc ttcctgaaat
    92581 gcaggaggat gaaatcctcg cgacggtggt cactgacagc ctgtgcctct cttcctggaa
    92641 agaggccaat ctgggtgaaa accataaaaa agtacccgac gatgtggcga ccaatcccat
    92701 catcatcggc cacgaatttt gcggcgatat tctggccgtg ggtaaaaagt ggcaacacaa
    92761 attccagccg ggtcagcgtt atgtgattca ggccaacctg caactccccg accgcccgga
    92821 ttgccccggc tactccttcc cgtgggtagg cggcgaagcg acgcatgtgg ttattcccaa
    92881 cgaggtcatg gaacatgatt gcctgctggc atacgacggc gaaacctatt ttgaaggctc
    92941 gctggttgaa ccgctttcct gcgtgattgg cgcgttcaac gccaactatc atcttcagga
    93001 aggtagttat aaccacacaa tggggattcg cccgcaaggg cgcacgctga tcctcggcgg
    93061 caccggtccg atggggctgc tggcgattga ttatgcgttg catggaccgg ttaacccgtc
    93121 gctgcttgtc attactgata ccgacaacga caaattgagt tatgcgcgca agcactatcc
    93181 atcagaaccg caaacactga ttcattatct caatgccgcc gatgcagcat ttgatacgtt
    93241 aatggcactg agtggcggtc acggcttcga tgatattttc gtctttgtgc ctaatgaagg
    93301 actggtgact ctcgcctctt ccttgctggc gacagatggt tgtctgaatt tcttcgccgg
    93361 accgcaggat aagcacttca gcgcgccaat taatttctac gatgtgcatt atgcatttac
    93421 ccactacgtg ggcacatcag gcggcaatac cgacgacatg cgcgcagcgg tcaaattgat
    93481 tgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc tgaatgccgc
    93541 gggcgaaacc acgcttgaat tgcctgccat cggcggcggc aaaaagctgg tgtataccgg
    93601 gaaatacctg ccgctgacgt cactcacgca gattcaggat caagcactgg cggtgattct
    93661 ggcgcatcat caggggatct ggtccggtga ggcggagcaa tacctgctcg cccatgcaga
    93721 ggcaatttcc catgattaat cgcgctactt aactctgcat gtcgctggct ggtcgccgcg
    93781 gcaacttcgc gacctgatgt gcggctgatt gctgaagcgg cacaatgtgc acgagaagaa
    93841 taaaaaaaca tcagcgcctt aaggggcgct caaattggtg gcaaagtgca cattgtttat
    93901 gccggatgcg gaatgaagcc ttattcgcct acataatctt gaaaattcaa tatattgcag
    93961 ttatctggta ggccaggtaa gcgtaacgca tcaggcaatc ctgtgtttgt catcagtctc
    94021 agcgcctgtg caatatgtga catcgtgagc catgtcagta agtggttaaa ttcgcagtgg
    94081 tagcattccc tccgtgtttt cttactaacc ggagaagtaa tggaactgct tttattgagt
    94141 aactcgacgc tgccgggtaa agcctggctg gaacacgcgc tgccgctgat tgctgaacag
    94201 ttgcagggtc gccgttctgc ggtatttatt cctttcgctg gcgtaacgca gacctgggat
    94261 gattacacag cgaaaacggc tgcggttctc gctccgctgg gtgtttctgt caccggtatt
    94321 catagcgttg tcgatcccgt tgccgcgatt gaaaatgcgg aaattgtgat tgtcggcggc
    94381 ggtaatactt tccagttgct gaaacagtgc cgcgagcgcg ggctgctggc accaattact
    94441 gacgtggtga aacgtggcgc gctgtatatt ggctggagcg caggcgctaa ccttgcttgc
    94501 ccaaccattc gtaccaccaa cgatatgccg attgtcgatc cgcaaggttt cgatgcgcta
    94561 aatctgttcc cgctgcaaat caacccgcac ttcaccaacg cgctgccgga aggccataaa
    94621 ggtgaaaccc gtgagcagcg tattcgcgaa ctgctggtcg tcgcgccaga actgacgatt
    94681 attggtctgc cggaaggtaa ctggatcaca gtaagtaaag gtcacgctac gctgggtggc
    94741 ccgaacacca cttatgtatt taaggctggt gaagaagcgg tgccgctgga agcgggtcac
    94801 cgtttttaag tgaattgccg gatacatgat ccggcatctt gttcatagtg cctgatgcga
    94861 cgctttacgc gtcttatcag gccttcaggg tcacggtatt aatactcgtc ccgtccttca
    94921 tcttcatccg gctgttccag cacactgtaa gccaccgaac aaaacagcga gttcagacgc
    94981 tgcatatctc ccagtaagcc taaatgtagc gaactggttt caatactctg cacgttttgc
    95041 tggtgcaggc gatcgacgtg ggcgtgggaa tagcggcgat taagaatgcg aaaacgatgt
    95101 ttgctgcggc gcaaacgacg ggcgctggta acatcgccag agaagaaaac cgacattgcc
    95161 agttttaaat tactgagcaa ttgctcatag agcgcatcca gttccttcaa gccatcaagc
    95221 gaaaatgctc gccttgctgc cagcgatttg tcggcaattt cactccccat gcgctcgacg
    95281 atatcggagg cctgttcaag gttgagcgac atttcgatga tctccgccca gcggcgcgac
    95341 tcttcttctg ccagttcctc ttttggcatc cgcgccagat acagcttaat ggcggtatag
    95401 agaacgttga tgtcatcagc cagcttacgc agctctttct cctgccgtgg ctcgccgtgc
    95461 atcactttat tcaacccttc catcatctgt tccatggcgt cgccaatgcg cagggtttcg
    95521 cgggcagcgt tcgccagagc aagcgtgggc gtatccagcg cgctgacatc cagatgttta
    95581 ggccgtagct gggtatccag ttccggttca tcgcgaataa tcgttttgca aaaccgtgcc
    95641 atcgggtcaa cgaatggcaa catgaccagg cagcgtacaa ggttgtagaa gacgtggaaa
    95701 tagatcacca attccgcttt tggcaatggc aacttcccca ttgtctctgc cagcaaatga
    95761 acaaacggca ggataatcag gctacccacc agcttaaaca gcaggctacc cagcgcgaca
    95821 cggcgggctg cggcattggc ggcactgttg ttgagcatcg ccagcaggcc ggaaccgagg
    95881 tttgcaccaa tcaccagaca aagcgccact gggaaagaga taatgcctgc ggcggtcaga
    95941 gtcgcggtca gcagtaccgc ggcaaggctg gagtagctga taatggcgaa catcgcacca
    96001 atcagcgcat ccagcaaaat gtcgcctgtc agcgaggcaa agatcacctg aacgccgttt
    96061 gcctgagtga tcggcgttac ggcctgcaca atcaactcca gcgccagtaa aatcagccca
    96121 agaccaatac cgacgcggcc cagctgcccg gcgcgtgact gtttgcgtcc gaggaaaaaa
    96181 atcacgccga taaaaataag taacggtgac agccaggata aatcgaaggt gagaatacgc
    96241 gccatcagcg ccgtcccgac atctgcaccc agcacaatca ccagcgcagg cgcgagagca
    96301 accagatcct gagcgacgaa cgaggtcacc agcatggtgg tggcattact gctctgcacc
    96361 agtgcggtaa cgccgatccc cgcgcaaaag gcgagcggct ttttttcgac gctccggcta
    96421 aggacagtac gcagacgcgc gccgaagacg cgcattacgc ctgttcgaac aatatgagtc
    96481 ccccagacca gcagggcgac ggcagaaagc aggtgaagca aagttagcac ggaatcaggt
    96541 tctccttttt gttatgcatt ttcctgttaa gatttttcta atcacgttgt ttttatccag
    96601 gcatttctct tgtatgtact ttcagtataa gagatttata caagaaagca tttaaaaagg
    96661 catttatata aaaatccacc tgtatctgtt gctatttctg tcacactttc ccctgtcgtt
    96721 tacaccagat aatcgttgtc acttttgtga cctc
//
LOCUS       DAZGJT010000003        87455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZGJT010000003 DAZGJT010000000
VERSION     DAZGJT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 87455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.003"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..434)
                     /locus_tag="ACHKFK_000186"
     CDS             complement(<1..434)
                     /locus_tag="ACHKFK_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9050620.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDE"
     gene            complement(505..2619)
                     /gene="fusA"
                     /locus_tag="ACHKFK_000187"
     CDS             complement(505..2619)
                     /gene="fusA"
                     /locus_tag="ACHKFK_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO9050621.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2716..3186)
                     /gene="rpsG"
                     /locus_tag="ACHKFK_000188"
     CDS             complement(2716..3186)
                     /gene="rpsG"
                     /locus_tag="ACHKFK_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO9050622.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3283..3657)
                     /gene="rpsL"
                     /locus_tag="ACHKFK_000189"
     CDS             complement(3283..3657)
                     /gene="rpsL"
                     /locus_tag="ACHKFK_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO9050623.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3783..4070)
                     /gene="tusB"
                     /locus_tag="ACHKFK_000190"
     CDS             complement(3783..4070)
                     /gene="tusB"
                     /locus_tag="ACHKFK_000190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO9050624.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGRISNDIISIDYTDFVRLTVKHSSQMAW"
     gene            complement(4077..4436)
                     /gene="tusC"
                     /locus_tag="ACHKFK_000191"
     CDS             complement(4077..4436)
                     /gene="tusC"
                     /locus_tag="ACHKFK_000191"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO9050625.1"
                     /translation="MKRIAFVFSTAPHGTTAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWICAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4436..4822)
                     /gene="tusD"
                     /locus_tag="ACHKFK_000192"
     CDS             complement(4436..4822)
                     /gene="tusD"
                     /locus_tag="ACHKFK_000192"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO9050626.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4822..5544)
                     /gene="yheO"
                     /locus_tag="ACHKFK_000193"
     CDS             complement(4822..5544)
                     /gene="yheO"
                     /locus_tag="ACHKFK_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9050627.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5711..6523)
                     /gene="fkpA"
                     /locus_tag="ACHKFK_000194"
     CDS             complement(5711..6523)
                     /gene="fkpA"
                     /locus_tag="ACHKFK_000194"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9050628.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKQEADAKAADSAKK"
     gene            6744..6962
                     /gene="slyX"
                     /locus_tag="ACHKFK_000195"
     CDS             6744..6962
                     /gene="slyX"
                     /locus_tag="ACHKFK_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO9050629.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(7011..7601)
                     /gene="slyD"
                     /locus_tag="ACHKFK_000196"
     CDS             complement(7011..7601)
                     /gene="slyD"
                     /locus_tag="ACHKFK_000196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9050630.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7696..7896)
                     /gene="yheV"
                     /locus_tag="ACHKFK_000197"
     CDS             complement(7696..7896)
                     /gene="yheV"
                     /locus_tag="ACHKFK_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO9050631.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7906..9711)
                     /gene="kefB"
                     /locus_tag="ACHKFK_000198"
     CDS             complement(7906..9711)
                     /gene="kefB"
                     /locus_tag="ACHKFK_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO9050632.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9711..10262)
                     /gene="kefG"
                     /locus_tag="ACHKFK_000199"
     CDS             complement(9711..10262)
                     /gene="kefG"
                     /locus_tag="ACHKFK_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO9050633.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFINIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGC"
     gene            10393..12306
                     /gene="yheS"
                     /locus_tag="ACHKFK_000200"
     CDS             10393..12306
                     /gene="yheS"
                     /locus_tag="ACHKFK_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9050634.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12306..13328
                     /gene="yheT"
                     /locus_tag="ACHKFK_000201"
     CDS             12306..13328
                     /gene="yheT"
                     /locus_tag="ACHKFK_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9050635.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13322..13540
                     /gene="yheU"
                     /locus_tag="ACHKFK_000202"
     CDS             13322..13540
                     /gene="yheU"
                     /locus_tag="ACHKFK_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO9050636.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13594..14463
                     /gene="prkB"
                     /locus_tag="ACHKFK_000203"
     CDS             13594..14463
                     /gene="prkB"
                     /locus_tag="ACHKFK_000203"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO9050637.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQSLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFS
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14518..14922)
                     /gene="yhfA"
                     /locus_tag="ACHKFK_000204"
     CDS             complement(14518..14922)
                     /gene="yhfA"
                     /locus_tag="ACHKFK_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO9050638.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15224..15856
                     /gene="crp"
                     /locus_tag="ACHKFK_000205"
     CDS             15224..15856
                     /gene="crp"
                     /locus_tag="ACHKFK_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO9050639.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15907..17997
                     /gene="yhfK"
                     /locus_tag="ACHKFK_000206"
     CDS             15907..17997
                     /gene="yhfK"
                     /locus_tag="ACHKFK_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO9050640.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQVLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVTLHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQDAIRLILSEYPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(18064..19284)
                     /gene="argD"
                     /locus_tag="ACHKFK_000207"
                     /gene_synonym="dapC"
     CDS             complement(18064..19284)
                     /gene="argD"
                     /locus_tag="ACHKFK_000207"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO9050641.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPVSAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEEGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MHRFAHAVAKVIGA"
     gene            complement(19370..19933)
                     /gene="pabA"
                     /locus_tag="ACHKFK_000208"
     CDS             complement(19370..19933)
                     /gene="pabA"
                     /locus_tag="ACHKFK_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO9050642.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGVGVFKGLANPLTVTRYHSLVVEPDSLPECFEVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19965..20567)
                     /gene="fic"
                     /locus_tag="ACHKFK_000209"
     CDS             complement(19965..20567)
                     /gene="fic"
                     /locus_tag="ACHKFK_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO9050643.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTLHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQNGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20557..20724)
                     /gene="yhfG"
                     /locus_tag="ACHKFK_000210"
     CDS             complement(20557..20724)
                     /gene="yhfG"
                     /locus_tag="ACHKFK_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO9050644.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEALRRHYER"
     gene            complement(20829..21401)
                     /gene="ppiA"
                     /locus_tag="ACHKFK_000211"
                     /gene_synonym="rotA"
     CDS             complement(20829..21401)
                     /gene="ppiA"
                     /locus_tag="ACHKFK_000211"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO9050645.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21672..22853
                     /gene="tsgA"
                     /locus_tag="ACHKFK_000212"
     CDS             21672..22853
                     /gene="tsgA"
                     /locus_tag="ACHKFK_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO9050646.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAYMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            23115..25658
                     /gene="nirB"
                     /locus_tag="ACHKFK_000213"
     CDS             23115..25658
                     /gene="nirB"
                     /locus_tag="ACHKFK_000213"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO9050647.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25655..25981
                     /gene="nirD"
                     /locus_tag="ACHKFK_000214"
     CDS             25655..25981
                     /gene="nirD"
                     /locus_tag="ACHKFK_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO9050648.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYDARVKDG
                     VVQLRG"
     gene            26107..26913
                     /gene="nirC"
                     /locus_tag="ACHKFK_000215"
     CDS             26107..26913
                     /gene="nirC"
                     /locus_tag="ACHKFK_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO9050649.1"
                     /translation="MFTDTITKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGTISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26932..28305
                     /gene="cysG"
                     /locus_tag="ACHKFK_000216"
     CDS             26932..28305
                     /gene="cysG"
                     /locus_tag="ACHKFK_000216"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO9050650.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEYGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28557..28724
                     /gene="yhfL"
                     /locus_tag="ACHKFK_000217"
     CDS             28557..28724
                     /gene="yhfL"
                     /locus_tag="ACHKFK_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO9050651.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            complement(29007..30155)
                     /locus_tag="ACHKFK_000218"
                     /pseudo
     CDS             complement(29007..30155)
                     /locus_tag="ACHKFK_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
     gene            complement(30167..30520)
                     /gene="yhfU"
                     /locus_tag="ACHKFK_000219"
     CDS             complement(30167..30520)
                     /gene="yhfU"
                     /locus_tag="ACHKFK_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO9050652.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(30531..31409)
                     /gene="php"
                     /locus_tag="ACHKFK_000220"
     CDS             complement(30531..31409)
                     /gene="php"
                     /locus_tag="ACHKFK_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO9050653.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYGFICQEMNDLM
                     ARGVRNVIEMTNRYMGRNAQFMLDVIRETGINVVACTGYYQDAFFPEHVATRSVEQLA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGCPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDFLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(31406..32632)
                     /gene="yhfW"
                     /locus_tag="ACHKFK_000221"
     CDS             complement(31406..32632)
                     /gene="yhfW"
                     /locus_tag="ACHKFK_000221"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9050654.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLETLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKMGRIVREQVQVGRVITFGGLLPDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(32633..33796)
                     /gene="yhfX"
                     /locus_tag="ACHKFK_000222"
     CDS             complement(32633..33796)
                     /gene="yhfX"
                     /locus_tag="ACHKFK_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO9050655.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRACLPVAHQ
                     GHLVQIPCHQVVDAVAQGTDVITVFTLDKAREVSAAAVEAGRVQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIQNLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNAPSATSCTSLPLLAEYGVTHTEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQRITETYLNAVDDSSIDYTL
                     PLAGEHPVSSAVVLCFRTQIFITRSDVVLVSGIHRGEPEIVGRYDSLGNPLEA"
     gene            complement(33880..34242)
                     /gene="yhfY"
                     /locus_tag="ACHKFK_000223"
     CDS             complement(33880..34242)
                     /gene="yhfY"
                     /locus_tag="ACHKFK_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9050656.1"
                     /translation="METRLNLLCDAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDDELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(34259..35164)
                     /gene="yhfZ"
                     /locus_tag="ACHKFK_000224"
     CDS             complement(34259..35164)
                     /gene="yhfZ"
                     /locus_tag="ACHKFK_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO9050657.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLTHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVILTRVDDYPMQQLL
                     HAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(35324..36328)
                     /gene="trpS"
                     /locus_tag="ACHKFK_000225"
     CDS             complement(35324..36328)
                     /gene="trpS"
                     /locus_tag="ACHKFK_000225"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO9050658.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGDIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASAHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(36321..37079)
                     /gene="gph"
                     /locus_tag="ACHKFK_000226"
     CDS             complement(36321..37079)
                     /gene="gph"
                     /locus_tag="ACHKFK_000226"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO9050659.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADILMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSLNDLLPALGLPHSENQESKND"
     gene            complement(37072..37749)
                     /gene="rpe"
                     /locus_tag="ACHKFK_000227"
     CDS             complement(37072..37749)
                     /gene="rpe"
                     /locus_tag="ACHKFK_000227"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO9050660.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(37767..38603)
                     /gene="dam"
                     /locus_tag="ACHKFK_000228"
     CDS             complement(37767..38603)
                     /gene="dam"
                     /locus_tag="ACHKFK_000228"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9050661.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVD
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(38710..39996)
                     /gene="damX"
                     /locus_tag="ACHKFK_000229"
     CDS             complement(38710..39996)
                     /gene="damX"
                     /locus_tag="ACHKFK_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO9050662.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKARNEEPEIEEELDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQIPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(40088..41176)
                     /gene="aroB"
                     /locus_tag="ACHKFK_000230"
     CDS             complement(40088..41176)
                     /gene="aroB"
                     /locus_tag="ACHKFK_000230"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO9050663.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLNTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(41233..41754)
                     /gene="aroK"
                     /locus_tag="ACHKFK_000231"
     CDS             complement(41233..41754)
                     /gene="aroK"
                     /locus_tag="ACHKFK_000231"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO9050664.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(42155..43393)
                     /gene="hofQ"
                     /locus_tag="ACHKFK_000232"
     CDS             complement(42155..43393)
                     /gene="hofQ"
                     /locus_tag="ACHKFK_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO9050665.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSVAWQNDNIA
                     RQEAEQTRAQANLPLENRNITLQYADARELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQGGGVGQVTT
                     LGSDLSVATATTHIGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(43305..43709)
                     /gene="hofP"
                     /locus_tag="ACHKFK_000233"
     CDS             complement(43305..43709)
                     /gene="hofP"
                     /locus_tag="ACHKFK_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO9050666.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPHWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(43699..44139)
                     /gene="hofO"
                     /locus_tag="ACHKFK_000234"
     CDS             complement(43699..44139)
                     /gene="hofO"
                     /locus_tag="ACHKFK_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO9050667.1"
                     /translation="MNMFFDWWFATSLRLRQFCWAVWLLVLVTLIFLSLTHHEESDAL
                     IRLRANHHQQWAALYRLVDTTSFSEEKTLPFSPLDFQLSGAQLVYWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFLLSVEGDDLLFTLQLETPHEG"
     gene            complement(44123..44662)
                     /gene="hofN"
                     /locus_tag="ACHKFK_000235"
     CDS             complement(44123..44662)
                     /gene="hofN"
                     /locus_tag="ACHKFK_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO9050668.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     NAEARVNAVLLQAEQQLARSLQITKPRLLERQQLREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSIAALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(44662..45441)
                     /gene="hofM"
                     /locus_tag="ACHKFK_000236"
     CDS             complement(44662..45441)
                     /gene="hofM"
                     /locus_tag="ACHKFK_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO9050669.1"
                     /translation="MAFKNWQIGLHLQQQEAVAVAIVRSAKECLLHRWWRLPLENDII
                     KDGRIVDVQRLANTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQMAWL
                     SGTMARELDMDPDSLRFDYSEDSLSLAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPES
                     VALCGEDGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKVY"
     gene            45561..48113
                     /gene="mrcA"
                     /locus_tag="ACHKFK_000237"
     CDS             45561..48113
                     /gene="mrcA"
                     /locus_tag="ACHKFK_000237"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO9050670.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVVLSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRHDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRGTGQLANGGNSREEYFIEGTQPTQLAVHEVGTTIIDNGEAQELF"
     gene            complement(48279..48839)
                     /gene="nudE"
                     /locus_tag="ACHKFK_000238"
     CDS             complement(48279..48839)
                     /gene="nudE"
                     /locus_tag="ACHKFK_000238"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO9050671.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVFEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     APDFNEARNVSALFLVREWLKGQGRV"
     gene            49159..51294
                     /gene="igaA"
                     /locus_tag="ACHKFK_000239"
     CDS             49159..51294
                     /gene="igaA"
                     /locus_tag="ACHKFK_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO9050672.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTSEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFMMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLRTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            51359..52027
                     /gene="yrfG"
                     /locus_tag="ACHKFK_000240"
     CDS             51359..52027
                     /gene="yrfG"
                     /locus_tag="ACHKFK_000240"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO9050673.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            52038..52439
                     /gene="hslR"
                     /locus_tag="ACHKFK_000241"
     CDS             52038..52439
                     /gene="hslR"
                     /locus_tag="ACHKFK_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO9050674.1"
                     /translation="MKEKPAVKVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            52464..53342
                     /gene="hslO"
                     /locus_tag="ACHKFK_000242"
     CDS             52464..53342
                     /gene="hslO"
                     /locus_tag="ACHKFK_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO9050675.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(53405..55129)
                     /gene="yhgE"
                     /locus_tag="ACHKFK_000243"
     CDS             complement(53405..55129)
                     /gene="yhgE"
                     /locus_tag="ACHKFK_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO9050676.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNLSWIVYGV
                     PATVAFSSVLLFSVISFKQNRLWGWLALVFIATLGMSDWLKWQTDGMTPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNLLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFKTLFFATDGFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTADRLQGALAVLVLLVWSLGY
                     FVSIVWRKGQNPLDLQGKVNLVVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAEYMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LADNVLIAPGSGKPDATFWSALIQDRYNVMTCIGKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSTRKEWDALDMSLLPRKITKEKLLTAAKDGKLGTRPKAWRDLVVDGE
                     RLDVNLNE"
     gene            55508..57130
                     /gene="pckA"
                     /locus_tag="ACHKFK_000244"
     CDS             55508..57130
                     /gene="pckA"
                     /locus_tag="ACHKFK_000244"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO9050677.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIIMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDSSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            57240..57557
                     /locus_tag="ACHKFK_000245"
                     /pseudo
     CDS             57240..57557
                     /locus_tag="ACHKFK_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493756.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            57616..57912
                     /gene="nadS"
                     /locus_tag="ACHKFK_000246"
     CDS             57616..57912
                     /gene="nadS"
                     /locus_tag="ACHKFK_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9050678.1"
                     /translation="MKDELFADLLASAEEMVRIEKGEETPEPEHVHTFSEIDVKAIRE
                     ATGLRQQDFAVAVGVSYDLVKSRETKRRQPTGAPRKLLLLLQANPFIINQLKTI"
     gene            complement(57943..59295)
                     /gene="envZ"
                     /locus_tag="ACHKFK_000247"
     CDS             complement(57943..59295)
                     /gene="envZ"
                     /locus_tag="ACHKFK_000247"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO9050679.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFVSLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGMTKEG
                     "
     gene            complement(59292..60011)
                     /gene="ompR"
                     /locus_tag="ACHKFK_000248"
     CDS             complement(59292..60011)
                     /gene="ompR"
                     /locus_tag="ACHKFK_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO9050680.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            60239..60715
                     /gene="greB"
                     /locus_tag="ACHKFK_000249"
     CDS             60239..60715
                     /gene="greB"
                     /locus_tag="ACHKFK_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO9050681.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            60812..63133
                     /gene="yhgF"
                     /locus_tag="ACHKFK_000250"
     CDS             60812..63133
                     /gene="yhgF"
                     /locus_tag="ACHKFK_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO9050682.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLANAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNDRPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            63571..63798
                     /gene="feoA"
                     /locus_tag="ACHKFK_000251"
     CDS             63571..63798
                     /gene="feoA"
                     /locus_tag="ACHKFK_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO9050683.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            63815..66136
                     /gene="feoB"
                     /locus_tag="ACHKFK_000252"
     CDS             63815..66136
                     /gene="feoB"
                     /locus_tag="ACHKFK_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO9050684.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGSRQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLVIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDVELLATRKSVSSCCAASTTGDCH"
     gene            66147..66383
                     /gene="feoC"
                     /locus_tag="ACHKFK_000253"
     CDS             66147..66383
                     /gene="feoC"
                     /locus_tag="ACHKFK_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO9050685.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLKQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLHEWWALR"
     gene            66616..67209
                     /locus_tag="ACHKFK_000254"
                     /pseudo
     CDS             66616..67209
                     /locus_tag="ACHKFK_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            complement(67238..68008)
                     /gene="bioH"
                     /locus_tag="ACHKFK_000255"
     CDS             complement(67238..68008)
                     /gene="bioH"
                     /locus_tag="ACHKFK_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO9050686.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEVVLRQAPDKAIWLGWSLGGLVASQIALTHPERVLAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPDEFCHLLVALKQRV"
     gene            68046..68729
                     /gene="gntX"
                     /locus_tag="ACHKFK_000256"
     CDS             68046..68729
                     /gene="gntX"
                     /locus_tag="ACHKFK_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO9050687.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPLPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLNARLRKHNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            68788..69363
                     /gene="nfuA"
                     /locus_tag="ACHKFK_000257"
     CDS             68788..69363
                     /gene="nfuA"
                     /locus_tag="ACHKFK_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO9050688.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            69727..71043
                     /gene="gntT"
                     /locus_tag="ACHKFK_000258"
     CDS             69727..71043
                     /gene="gntT"
                     /locus_tag="ACHKFK_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9050689.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(71154..73238)
                     /gene="malQ"
                     /locus_tag="ACHKFK_000259"
     CDS             complement(71154..73238)
                     /gene="malQ"
                     /locus_tag="ACHKFK_000259"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO9050690.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGKQYKGHVMGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESSSPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWKLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMADPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(73248..75641)
                     /gene="malP"
                     /locus_tag="ACHKFK_000260"
     CDS             complement(73248..75641)
                     /gene="malP"
                     /locus_tag="ACHKFK_000260"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO9050691.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRGNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLKKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQSDRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            76264..78969
                     /gene="malT"
                     /locus_tag="ACHKFK_000261"
     CDS             76264..78969
                     /gene="malT"
                     /locus_tag="ACHKFK_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO9050692.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICDTMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGSPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTSDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFESAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(79012..80028)
                     /gene="rtcA"
                     /locus_tag="ACHKFK_000262"
     CDS             complement(79012..80028)
                     /gene="rtcA"
                     /locus_tag="ACHKFK_000262"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO9050693.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGVHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNSLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLQTNIAVVERFLPVRFSLIETDGVTR
                     VSIE"
     gene            complement(80032..81258)
                     /gene="rtcB"
                     /locus_tag="ACHKFK_000263"
     CDS             complement(80032..81258)
                     /gene="rtcB"
                     /locus_tag="ACHKFK_000263"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO9050694.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVKQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            81447..83044
                     /gene="rtcR"
                     /locus_tag="ACHKFK_000264"
                     /pseudo
     CDS             81447..83044
                     /gene="rtcR"
                     /locus_tag="ACHKFK_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(83026..83784)
                     /locus_tag="ACHKFK_000265"
     CDS             complement(83026..83784)
                     /locus_tag="ACHKFK_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO9050695.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDTMPPASVMQVLKDHHIQLELC"
     gene            complement(83801..84631)
                     /gene="glpG"
                     /locus_tag="ACHKFK_000266"
     CDS             complement(83801..84631)
                     /gene="glpG"
                     /locus_tag="ACHKFK_000266"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO9050696.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLGNPADPRYLAASWQSGHTDSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(84676..85002)
                     /gene="glpE"
                     /locus_tag="ACHKFK_000267"
     CDS             complement(84676..85002)
                     /gene="glpE"
                     /locus_tag="ACHKFK_000267"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO9050697.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAAQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            85192..86697
                     /gene="glpD"
                     /locus_tag="ACHKFK_000268"
     CDS             85192..86697
                     /gene="glpD"
                     /locus_tag="ACHKFK_000268"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO9050698.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIMRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARREKGLWIVEAEDIDTGKKYTWQARGLVNATGPWVKQFFDDGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLNRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDEDGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGENFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLMEYTQQRLSLAS"
     gene            complement(86751..87356)
                     /locus_tag="ACHKFK_000269"
     CDS             complement(86751..87356)
                     /locus_tag="ACHKFK_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050699.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIALAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELLVEYALFFSVMGGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCQWRSKNRSHRKKWGDWVTCLSLAILALGPLFNYLQ"
BASE COUNT        21166 a        23682 c        21945 g        20662 t
ORIGIN      
        1 tcttcgtcat caaccatgtc gcatttgttc aggaacacga tgatgtacgg aacgcctacc
       61 tgacgaccca gcaggatgtg ctcacgagtc tgcggcatcg ggccgtcagt cgcagcaact
      121 accaggatcg cgccgtccat ctgagcagca ccggtgatca tgtttttaac atagtcggcg
      181 tgccccgggc agtctacgtg cgcgtagtga cgggtcgggg tgtcgtattc aacgtgagaa
      241 gtgttgatgg tgataccacg agctttttct tccggcgcgt tatcgatctg gtcgaatgca
      301 cgagcagcac cgccgtaggt tttagccagt acggtggtga ttgcagcggt cagagtagtt
      361 ttaccgtggt caacgtggcc gatagtacca acgttaacgt gcggttttgt acgttcaaat
      421 ttttctttag acacggctat attccttact atagtgctct ccccttcagg agagagcacg
      481 ggactttggt attaaccctt aggcttattt accacgggct tcaattacgg cctgagcaac
      541 gttactcggc gcttcatcat acttcaggaa ttccatagtg tatgatgcgc gacctttggt
      601 cagagaacgc agctgagttg catatccgaa catttcagac agcggtactt cagcgtggat
      661 cttaacgcca gtaacttcag attcctgacc tttgagcata ccacgacgac ggctcaagtc
      721 accgataacg tcaccggtgt tctcttcagg agtttctact tcaaccttca tgatcggctc
      781 aagcagaact ggtttcgctt tcttaaagcc ttctttaaag gcgatagaag cagccagttt
      841 aaacgccagt tcagaggagt caacgtcatg gtaagaaccg aagtgcagac gaatacccat
      901 gtctactacc gggtagcctg ccagcggacc tgctttcagc tgttcctgga tacctttatc
      961 aacggccggg atgtattcgc cagggattac accaccttta atgtcgttga tgaactcgta
     1021 gcctttcggg tttgaacccg gctccagcgg gtacatgtcg ataacaacat gaccatactg
     1081 accacgacca ccagactgtt tcgcgtgttt accttcaaca tcggtaactt tctggcggat
     1141 agtttcacgg taagcaacct gcggtttacc tacgttcgct tcaacgttga attcacgctt
     1201 catacggtca acgatgatgt cgaggtgcag ttcgcccata cccgcgatga tggtctggtt
     1261 agattcttcg tcagtccata cacggaaaga cgggtcttct ttagccagac ggcccagagc
     1321 cagacccatt ttttcctggt cagctttggt tttcggttca actgcgatgg agattaccgg
     1381 ctcagggaat tccatacgtt ccagaatgat cggcgcatcc gggtcacaca gggtgtcacc
     1441 agtggttacg tctttcagac cgattgcagc tgcgatgtcg cccgcgcgaa cttctttgat
     1501 ctcttcacgt ttgttagcgt gcatctgaac gatacgaccg aaacgctcac gtgcagcttt
     1561 cacggagttc agtacggtat caccagagtt aaccacaccg gagtacacac ggaagaaggt
     1621 caggttacca acaaacgggt cggtagcgat tttgaacgcc agtgcagaga acggctcgtc
     1681 atcacttgcg tgacgctcag ccggagtgtc tttaccgtcg tccaggatac cgttgatcgc
     1741 aggtacgtca accggggatg gcaggtaatc aattaccgca tccagcatcg cctgaacacc
     1801 tttgttcttg aacgcagaac cacaggttac caggatgatt tcgttgttca gaacgcgctg
     1861 acgcagagca cctttgattt ctgcttcagt cagttcttca ccacccaggt atttttccat
     1921 cagctcttca gaagcttcag ctgcggattc gatcaggttc tggtgccatt cgttagccag
     1981 ttcaaccatg tctgccggga tatcttcgta ttcgaaggtt acgccctggt cagcgtcgtt
     2041 ccagttgata gctttcattt tcaccaggtc aacaacaccg gtgaaatgtt cttcagcacc
     2101 aatcgccagc tgcagcggaa ccgggttcgc gcccagacgg gttttgatct ggttaacaac
     2161 tttcaggaag ttcgcaccca tgcggtccat tttgttaacg aacgcaatac gcggaacttt
     2221 atatttgttt gcctgacgcc atacggtttc agactgcggc tgaacaccac caactgcgca
     2281 gtaaaccatt accgcaccat cgagaacacg catggaacgt tctacttcga ttgtgaagtc
     2341 aacgtgcccc ggggtgtcga tgatgttgat gcgatgcggc tcatactgct tagccatacc
     2401 agaccagaat gcagtagtcg cagcggaagt gatggtaata ccacgttcct gctcctgctc
     2461 catccagtcc atggttgcag cgccgtcatg aacttcaccg attttatggt ttacaccggt
     2521 gtagaacaga atacgttcgg tagtagtggt tttaccggcg tcgatgtgcg cactgatacc
     2581 gatgttacgg tagcgtgcga tgggtgttgt acgagccatt tgtttcctcg tttatctttt
     2641 aggcgttcaa tttaagtagc ccaaagcggg ctgcttactg gaagcgcccg cctggtgact
     2701 aaaactccga agggattacc aacggtagtg tgcgaacgcc ttgttggctt cggccatacg
     2761 gtgaacgtct tcacgtttct taactgcagt acctttgttt tctgcagcat cagaaagttc
     2821 gttcgccagg cgcagagcca tggatttatc accgcgttta cgagcagctt caacgatcca
     2881 acgcattgcc agagcattac gacgaaccgg acggacttca actggtacct gataagtaga
     2941 accaccaacg cggcgagact taacttctac agtcgggcgc acgttttcga gagctacttc
     3001 gaatgcttcc agttcagatt taccagagcg ctgagccagg gtctccagcg cgctgtatac
     3061 gatagattca gcagtagatt ttttaccatc taccatcagg atatttacaa atttagccag
     3121 cagttctgat ccgaacttcg gatccggcag aattttacgc tgaccaatga cgcgacgacg
     3181 tggcatggaa atactccgtt gttaattcag gattgtccaa aactctacga gtttagtttg
     3241 acatttaagt taaaacgttt ggccttactt aacggagaac cattaagcct taggacgctt
     3301 cacgccatac ttggaacgag cctgcttacg gtctttaacg ccggagcagt caagcgcacc
     3361 acgtacggtg tggtaacgaa cacccgggag gtctttaaca cgaccaccac ggatcaggat
     3421 cacggagtgc tcctgcaggt tgtgaccttc accaccgatg taggaagtca cttcgaaacc
     3481 gttagtcaga cgaacacggc atactttacg cagcgcggag ttcggttttt taggagtggt
     3541 agtatataca cgagtacata cgccacgttt ttgcgggcat gcttccagcg caggcacgtt
     3601 gcttttcgca actttgcgag cacgtggttt gcgtaccagc tggttaactg ttgccattaa
     3661 atagctcctg gttttagctt ttgcttcgta aacacgtaat aaaacgtcct cacacaatat
     3721 gaggacgccg aattttaggg cgatgccgaa aaggtgtcaa gaaatataca acgatcccgc
     3781 catcaccagg ccatctggct ggagtgctta accgtaagtc tgacgaaatc agtatagtca
     3841 atggaaatga tgtcgttcga aattcgacca gtcaaaccgc gggcaataag gtcttcgttc
     3901 agagcataga ccttaatggg ggcattacgc agactttcaa ggtagcggtt gccgtcaact
     3961 gcggcagtta cgccatcttg caataatagc agttcgtcac cttcactgag cagacgcagc
     4021 agcgcagcaa agtccgtcag ccagggtgaa cgatgtaatg tgtgcagcat aacgcctcaa
     4081 aacctcaaaa taacatcgta gttggcgagt tcgcggcgta aggcatctgc ttcgagcggc
     4141 gtggcttcga ccacaaaggg tgtctgcgga tctaacccac gttcgcgcag tgaagccgcg
     4201 caaatccagc actgttcaat atcgtacaga cccaacaatt taaaagtggc aatgtaatca
     4261 cgcgccagca ctgcatcggg cttttgtcct ggcagcagct gaaaaacgcc atcagcaata
     4321 aagaagacgg ccagatcgtc agttaatgcg gaagttgcca gtaaagcatc taaaccttcc
     4381 cggcctgcag ttgtaccatg aggcgcagta gaaaaaacaa acgcaattcg tttcatcaga
     4441 actgtaccac cctgtcacag gtcagcgagg cttccgccag cgcgccaagt ccgcttaagg
     4501 taaatcccgg ctgaaggttt gacgaagcga gccccagtct tccggcctcc gtttcatcaa
     4561 caacgccacg gcgtaatgct gccgctacgc agatattcag cgccacacca tgttgcgcat
     4621 tcaactgttg ccagccgcgc acaaggtcaa attcgtcgct tgccggtgag gtcaattggt
     4681 tagcgttata gaccccttcc cgatagaaaa aaacgctgct taactcatgg ccttctgcta
     4741 tcagcgcctg cgcaaactga aaagcactac tcgcctgttg cgtaccgtat gctggcccgg
     4801 tcaccacgat ggcaaaacgc attacttatc ttgcccctgg aaatcaccgc tcttgaactg
     4861 gcggatgtag agatagacag tgtgtttgga gatgttcagg cggtcagcaa cctggttgat
     4921 cgcgtcttta atatcgaaga tccctttctc gtagagattt agcacgatct gacgattttt
     4981 ggcgttatta gaaacattgc gatcggcatt cacttcttcg atggtgaact ccagcgtttg
     5041 ggtaaccaga tcttcaacag aagaggcaaa gttgacgctt gaaccgacat ccggagtttc
     5101 cggcggcaca aaagtgctca taatctgcga gaagggaaca tcaagattca tattgatgca
     5161 cagcagacca attacacgct gttcgcggtt acgaatcgcg atagtcaggg acttcattaa
     5221 tacgccgctt ttggcgcgag taaagtagca tttagaaacg ctgctatccg ctcccgtcat
     5281 atcgtgcagc atacgtagcg ccaggtcagt aattggcgaa ccaatcttcc ggcctgtatg
     5341 ttcaccgtta gcaatacgaa tggctgaaca ttttagatcc tgcaaagagt gcaaaacgat
     5401 ttcacagtgg gagccaataa gcatcgctaa cccgtccacc accgcttcgt aggatttcag
     5461 aatatcaaaa tcggtctggt caaaaggacg ttgatccagt aaatccaact cactggtttc
     5521 gttggttaaa agcgacctgg acatgaaaaa aaacactcct tttcaggagc ctgtcgttaa
     5581 cttttcaggg caggctcatt aatgatgcgg gtaactaaat taatacagcg gaggttccgc
     5641 tttccagcac taattatatc cggcctgtaa taaaaaaacc gccgcctggt caggcggcgg
     5701 ttcttaatgc ttatttttta gcagaatctg cggctttcgc atcagcttcc tgctttgcat
     5761 cagccttcgg cgctggtttc acatccagca gctctacgtc aaacaccagg gtagaattcg
     5821 gtgggatccc cggaacaccc gctttgccgt aagccagttc tggtggaata accagtttga
     5881 tcttaccgcc tttcttgatg ttcttcagac cttctgtcca acccgggata acaccgtcca
     5941 gacggaaaga gagcggttca ccacgggtgt aagagttgtc gaactcttta ccgtcgatca
     6001 gcgtaccttt gtagttcact acaacagtat cgctgtcttt cggtgcttcg cctttaccgg
     6061 cttctactac ctgataaacc agaccagtgg aagaggtttt cacacctttc tctttggcaa
     6121 atttctcgcg gtactcttta ccttttgctt cgttatcagc cgcgtctttt tccatcttcg
     6181 cctgagcaga agacttcaca cgagcttcga atgcttgcag agtctgttcg atctcttggt
     6241 cggaaagttt gctcttatca gcaaatgcat cctgaacacc agcgatcagc tgatctttat
     6301 ccagtttgat gcccagtttt tcttgttctt taagagagtt ttccatgtaa cgacccagcg
     6361 aagcacccag tgcataagct gatttctgat cgtcattttt gaacgctgct ttgctgtcag
     6421 cagttgtagc aggttttgca gcttcagcag caaaagtgat tggtgcatgc agggcaacgg
     6481 ccattgtggt cgccagcagc gttactttaa acagtgattt catccatatc tccaggatcg
     6541 gggcatctca ccccagggtt aactattatc agaagggtac tataaagcgt tgtcgaacaa
     6601 atctacatac agacacgccc tattatcatc tattttcaga ctctttttgt ttaaattagt
     6661 ttcgatgacc gcgaaatgag tgctgtctcg ggcagcaaag ttaagtagaa tccgcggcga
     6721 ccattcgaca aaagaggtga atcatgcagg atttatcatt ggaagcacgc ctggcagagc
     6781 tggagagccg attggctttt caggaaatca ccattgaaga actgaacgtc acggtgaccg
     6841 ctcatgaaat ggagatggcg aaactgcgcg atcatctgcg tctgctgacc gagaagctaa
     6901 aagccagcca gccgtcgaac atcgcgtcgc aggctgaaga aacgccaccg ccacattatt
     6961 gaggcgtaaa aaaagcgggg attccccgct tttttgtcac cttttcggta ttagtggcaa
     7021 ccgcaaccgc cgttgccttt accgccacag cagccttcgc caccgtgttc atgaccgtga
     7081 tcgtggccat gaccaccgca gcaaccgtcg tggtcgtgat cgtggtggtg atcgtgcgcg
     7141 ccgtgaacgt gaccatgagc cagttcttct tcggtcgctt cgcgaatcgc cacaacttca
     7201 acgttgaatt tcaggttctg accagccagc atgtggttac catcaaccac tacgtgatcg
     7261 tcttcaaccg cagtgatttc aaccggtacc ggaccctggt cggtttcagc caggaaacgc
     7321 atacctacct gcagttcatc aacgcccata aatacgtctt taggaacgcg ctgcaccagg
     7381 ttttcgtcgt actgaccgta agcgtcgttc gcgccaacag cgacatcaaa tttgtcgcca
     7441 acttcatgac cttccagcgc tgtttccagg ccagagatca gggaaccgtg accatgcagg
     7501 tagtccagcg gcgcactcac cggagactca tcaaccaaca caccgtcttc tgtacgtacc
     7561 tgataggcca ggctgaccac caggtctttt gctactttca tgatatctcc tgagcatggg
     7621 aagaatagtg gcgcagattg tagcggaatt ctgcagccgt gtactcacta gcttaaaaaa
     7681 actcggcgca tatcgctagt ccggatgaaa aatcccgatc acttgctcat ctttgcggac
     7741 gtgatcgcgg gcttctttgt ctgcttctcg catctgatgt ccacacttaa cacattctac
     7801 gatatcaata ttattttcgc gccacatcgc cattgaatcc tgtgcctgac aggccgggca
     7861 ttttgcgccc gcaataaaac gttttcggat tgccatcttt accctctact caaattcatc
     7921 ccagccgtcc agctggcgtc gttcttgttg catttcacgc tggaaaatct cttccagttc
     7981 acgtcgggct tccctggcgc gagaaatttg tacggtatca gcatgcatcg ggattaactc
     8041 ccgcagcatt cgcatatcca ggcggcgaaa atgcagttgc gcgcgctgcg cctgatgcgg
     8101 atgcatgcca agcgtgacca gcgtcttgcg ccccagctct aacgcactgg agaatgtttc
     8161 acgggaaaac tgcgtcaccc ctgcctgtaa taactcatgc gcttccacac gtccgcgcgc
     8221 tcgcgcaaga atatgcaaat gcggaaagtg ctgctggcat atttccacca gcttcatagt
     8281 gtcttccggc tcgttacagg taatgacgat agactcagcg gcctctgcac ccgcagaacg
     8341 taaaagatct acctgcgtgg cgtcgccgta ataaactttg tagccatatt tgcgcatcag
     8401 gttaacggca ctgatatccc gctccagtac agtaatgcgc attttatttg ccatcagcaa
     8461 acgaccaatc acctgaccaa aacgcccaaa gcccacgaca atgacctgcg gtttatcatc
     8521 gttgacccat ggtttttcgt cttcttcttc cggtccgtta aactggcggg atagccattt
     8581 atcgaccagc ttcatcagca gcggcgtggt catcatggaa agcgtcaccg tcaccagcaa
     8641 cagcgccatc tggtcgccct ggaataagcg ttgtgaagaa gcggtagaaa agaggacaaa
     8701 ggcaaactca ccgccctgac tcaacacgcc agcaaactgc atccgctcag aactgcgcac
     8761 gccatacaat cgcgccagca gatacagcac gagaattttc accgccacca gcacaaccac
     8821 acttatcact acccacaaca gatgggtata aagcaccccg aggttgagcg acatgccgac
     8881 agagataaag aacaaaccaa gcagcaagcc tttgaaggga tcgatagccg tttccagttc
     8941 atggcgatat tcactttccg ccagtagcac acccgcaata aacgtaccga gcgccatcga
     9001 caaccccagc gcatccataa acaatgcgga acccaacacc agcagcagcg tcgcggcggt
     9061 gaacacttcc cgcacaccag aagctgcaat aaaacggaat accggacgca gtaaatagcg
     9121 cccgccaatc agcatgccga caaacgccag caccttcatg ccgatcttca tccagtcgaa
     9181 atgttcgtct gccgaacccg ccaacaacgg cactaacgcc agtgccggga tcaccgccag
     9241 atcctgaaac agcagtaccg agaaacctaa ctggccggat tcgctgcgat tcatcccttt
     9301 ctcacgcatc aattgcaacg ccattgcagt tgaagacatc gcaaggccaa tgccaccgac
     9361 caccgccgcc tgccatgaga aatgggtcag catcaataat cctgccagca acgccgcgct
     9421 taacagcacc tgtgccgcgc ctacgccaaa aatcgaacgc cgcagttgcc aaagcttgga
     9481 gggattcaac tcaaggccga tgataaacat caggaatacc acgccgagtt ccgaaaagtg
     9541 gaggatctca tcgacgtcgc taataaaccc cagcccccac gggccaatcg caatccccgc
     9601 cagcaaatat cccaacactg caccaatacc cagccgcgat gccagcggca ccgcagccac
     9661 cgccgcgaag agaaacagca ctcctgcgag taaaaaatca gaaccttcca tcagcagcct
     9721 cctggagaca gcggatttgc cagccagtca ccgtaggctc tggcgtggct cgccagctcc
     9781 tgtgcgcttt gccgtctcgc ccagtaaata atgatggggc ttaaccaatg catccggcac
     9841 atgcccgccg ccagttcaaa ggggcgcaga acatcgctca tcgggtagcg attcaacgcg
     9901 tcataacggt aagcactttc cggctccccg gtggtaatca cgctacgcca gtactttccc
     9961 gccagttggt ttccccccgg tccgctggca aaaccacggc ttaatacccg gtccagccac
    10021 tctttcagta gcgccgggca gctataggta taaagaggat gctgaaagac aatcacctcg
    10081 tgctcgcgca gtaatgcctg ctcacgaggg atattaataa aaaagtcggg atagtgcgcg
    10141 taaaggtcgt gcacggtaac attgctgagc tgcgtggccg gtttaagcag aacccggttt
    10201 gccaccgagt cctgagattc cggatgggca tacagcagca aaactttcgc tggctgagac
    10261 atcatccccc tcccggtatg gtttttgtgt atagtcgctg ttttgggcta ccattgcgcc
    10321 cggtgcggca gctcgcccat acattacatt atcataatga taagttaaca tagtctgaac
    10381 atacggcgcc ttatgattgt tttttcctcg ttacaaattc gtcgcggcgt gcgcgtcctg
    10441 ctggataatg ccaccgccac catcaaccct gggcagaaag tcggcctggt gggtaaaaac
    10501 ggctgtggta aatctaccct gctggcattg ctgaaaaatg aaatcagcgc cgacggcggc
    10561 agctacacct ttcctggaag ctggcaactg gcgtgggtga atcaggaaac accggcgtta
    10621 ccgcaagcgg cgctggaata tgtcattgac ggcgaccgtg aatatcgtca actggaagcg
    10681 cagctacacg acgccaacga acgtaacgac gggcacgcca ttgcgaccat tcatggcaag
    10741 ctggatgcta ttgacgcatg gagtattcgc tcccgtgccg ccagcctgct gcacggcctc
    10801 ggtttcagca atgaacaact ggagcgcccg gtaagtgatt tctccggtgg ctggcgtatg
    10861 cgtcttaacc ttgcccaggc gctgatttgc cgttcagact tgctgctgct cgacgaaccg
    10921 actaaccacc tcgatctcga tgccgttatc tggctggaaa aatggctgaa gagctatcag
    10981 ggcacgctga tcctgatctc tcacgatcgc gatttcctcg atccgatcgt tgataaaatt
    11041 attcatatcg aacaacaaag catgttcgag tacaccggca actacagttc gtttgaagta
    11101 cagcgcgcca cccgtctggc gcagcaacaa gcgatgtatg aaagccagca ggaacgcgta
    11161 gcgcatctgc aaagttatat cgaccgtttc cgtgccaaag ccaccaaagc gaagcaggcc
    11221 cagagccgca ttaagatgct tgagcgtatg gagctgattg cccccgcgca cgtcgacaac
    11281 ccgttccgct ttagcttccg cgcgccggaa agcctgccaa atccgttact gaagatggaa
    11341 aaagtcagcg caggctatgg cgatcgcatt attctcgact cgattaaact gaatctggtc
    11401 cccggctcgc gcattggtct gctaggccgc aacggcgcgg gtaaatcgac attaatcaaa
    11461 ctgttagccg gggaacttgc gccagtcagc ggtgaaattg gtctggcgaa agggatcaag
    11521 ctcggctact tcgcccagca tcaactggaa tacctgcgcg ccgacgaatc gcctattcaa
    11581 catctggcac gtttagcgcc gcaggagctg gaacaaaaac tgcgtgacta cctcggcggc
    11641 tttggtttcc agggcgataa agtaaccgaa gaaacgcgcc gcttctccgg tggggaaaaa
    11701 gcccgcctgg tgctggcatt aattgtctgg cagcgtccga atctgctgct gctcgacgaa
    11761 ccgaccaacc accttgacct cgacatgcgt caggcactca ccgaagcatt aatcgagttc
    11821 gaaggcgcgc tggttgtcgt ctcgcacgac cgtcatttgc tgcgttccac cactgacgat
    11881 ctctacctgg ttcacgatcg taaagtcgaa ccgttcgacg gcgatctgga agattatcaa
    11941 cagtggttga gcgacgtaca aaagcaggaa aaccagaccg acgaagcgcc aaaagagaac
    12001 gcgaacagcg cccaagcacg taaagatcag aagcgtcggg aagcggagct gcgtgcgcaa
    12061 acccagccac tgcgtaaaga gattgcccgt ctggaaaaag agatggagaa gctgaacgcg
    12121 caactggcgc aggcggaaga gaaactcggc gacagcgaac tgtacgatca aagccgtaaa
    12181 gcggagttga ccgcctgcct gcaacagcaa gccagcgcca aatccggcct ggaagagtgc
    12241 gaaatggcat ggctggaagc ccaggagcag cttgagcaga tgctgctgga aggccaaagc
    12301 aactgatggc gcagataacg acgaccgatg ccaatgaatt cagcagcagt gctgaattca
    12361 cccccatgcg cggctttagc aattgtcatc tacaaaccat gctgccgcgt ctgttccgtc
    12421 gcaaggtgaa attcaccccg tactggcagc ggctggagtt gcccgacggc gattttgtcg
    12481 atctcgcgtg gagtgaagat cctgcacagg cgcggcataa accgcgttta gtggtatttc
    12541 acgggctgga aggcagtctc aatagccctt acgctcacgg tctggttgag gcggcgcaaa
    12601 agcgcggctg gctgggcgtg gtgatgcatt ttcgcggatg cagcggtgaa ccaaaccgta
    12661 tgcaccgcat ttaccattcg ggcgaaaccg aagacgccag ttggttttta cgctggctgc
    12721 aacgcgagtt tggtcatgcg ccaacggctg ccgtcggcta ttcgctcggc ggtaatatgc
    12781 tggcctgttt gctggcaaaa gaaggcaatg atctcccgat tgatgcggca gtgattgtct
    12841 ctgcgccgtt tatgctggag gcctgtagtt accatatgga aaagggcttt tcccgcgttt
    12901 atcagcgtta cttgctgaac ctgttaaaag ccaatgccgc gcgcaagctg gcagcctacc
    12961 ccggcacact gccgattaat ctcgcgcagt taaaatcggt acgtcgcatc cgtgaatttg
    13021 acgatctcat caccgccaga attcacggct acgctgacgc tatcgactat tatcgtcagt
    13081 gtagcgccat gccgatgctc aatcggatag ccaaaccgac actgattatt cacgccaaag
    13141 acgatccgtt tatggatcat caggtgatcc cgaaaccgga aagtctcccc ccgcaggtgg
    13201 agtatcagct gactgaacat ggcggtcatg ttggctttat tggcggtaca ttacttcatc
    13261 cgcaaatgtg gctggagtca cgcattcctg actggttaac aacgtatctg gaggcgaaat
    13321 catgttgatt ccgtggcaag acctctcccc cgaaacgctg gaaaatttaa ttgaaagctt
    13381 tgtgttacgt gaaggcaccg attatggtga acatgagcgt acacttgaac agaaagtcgc
    13441 cgacgtcaaa cgccagctac agtgcggaga agcggtgctg gtatggtcgg agctgcacga
    13501 aacggtcaat atcatgccgc gcagtcagtt tcgcgaataa cctgcaaccg tcggcggacg
    13561 agataataat gatagctaag tcatggagtt actatgtctg ccaaacatcc ggtcattgcg
    13621 gtaacaggat ccagcggcgc ggggaccacc accaccagcc tcgcgtttcg taaaatattc
    13681 gcgcagttaa atctgcatgc agccgaggtg gaaggcgaca gttttcaccg ttatacccgc
    13741 ccggaaatgg acatggcgat ccgcaaagcg cgcgacgccg ggcggcatat cagctacttc
    13801 ggccccgaag cgaacgactt cggccttctg gaacaaacct tcattgaata cggtcagagc
    13861 ggcaaaggga aatctcgcaa atatctgcat acctacgatg aagccgtacc gtggaatcag
    13921 gtaccgggga cattcacccc ctggcaatct ttaccggaac ccactgatgt actgttttat
    13981 gaaggtttac acggcggcgt agtcacgcca cagcataacg ttgcgcagca tgtggactta
    14041 ctggtcggcg tggtgcctat cgttaacctt gagtggattc aaaaactgat ccgtgacacc
    14101 agcgagcgcg ggcactcacg agaagcagtg atggactcgg tagtgcgttc aatggaagac
    14161 tatatcaact acatcacacc gcagttttcc cgcacccatc ttaacttcca gcgcgttccc
    14221 accgtcgaca cctcaaaccc gttctcggca aaaggtatcc cgtcactgga tgaaagcttt
    14281 gtggtgatcc attttcgtaa tctggaaggg atcgatttcc cctggctgct ggcgatgttg
    14341 caaggctcat tcatttccca catcaatacg ttagtggtgc ctggcggcaa aatgggtctg
    14401 gcaatggaat taattatgct gccgctggtg caacgattga tggaaggaaa gaaaatcgag
    14461 taacgctgct attacgccgg ataaaatact atccggcttc acaacgggat agttaaatca
    14521 cgcggcaacc acttcatacg agtgagtaat attcaccgct ttttccagca tcagcgccac
    14581 tgagcaatat ttctcggcag aaagatcaac cgcacgcgca accgctgcgt ctttcagatc
    14641 gcgaccggtg acgataaaat gtagattaat gtgcgtaaac aggcgtggtg cctcttcgcg
    14701 gcgctcagag gtcaatttta cttcacaatc gaccacatcc tgacgccctt tttgcaggat
    14761 agaaaccaca tcgatggcac tgcaaccacc cgccgccatc aacaccattt ccatcgggct
    14821 tggtgcttta tcgcctgagt tgccgtccat taaaatctgg tgaccagagg cggattcgcc
    14881 cagaaaagtt aacccttcga cccacttcac tcgcgcttgc atttttgcta ctccactgcg
    14941 tcaattttcc tgacagagta cgcgtactaa ccaaatcgcg caacggaagg cgacctgggt
    15001 catgctgaag cgagacacca ggagacacaa agcgaaagct atgctaaaac agtcaggatg
    15061 ctacagtaat atattgatgt actgcatgta tgcaaaggac gccacattac cgtgcagtac
    15121 agttgatagc cccttcccag gtagcgggaa gcatatttcg gcaatccaga gacagcggcg
    15181 ttatctggct ctggagaaag cttataacag aggataaccg cgcatggtgc ttggcaaacc
    15241 gcaaacagac ccgactctcg aatggttctt gtctcattgc cacattcata agtacccatc
    15301 caagagcacg cttattcacc agggtgaaaa agcggaaacg ctgtactaca tcgttaaagg
    15361 ctctgtggca gtgctgatca aagacgaaga gggtaaagaa atgatcctct cctatctgaa
    15421 tcagggtgat tttattggcg aactgggcct gtttgaagag ggccaggaac gtagcgcatg
    15481 ggtacgtgcg aaaaccgcct gtgaagtggc tgaaatttcg tacaaaaaat ttcgccaatt
    15541 gattcaggta aacccggaca ttctgatgcg tctgtctgca cagatggcgc gtcgtctgca
    15601 agtcacttca gagaaagtag gcaacctggc gttcctcgac gtgacgggcc gcattgcaca
    15661 gactctgctg aatctggcaa aacaaccgga tgctatgact cacccggacg gtatgcaaat
    15721 caaaattacc cgtcaggaaa tcggtcagat tgtcggctgt tctcgtgaaa ccgtgggacg
    15781 cattctgaag atgctggaag atcagaacct gatctccgca cacggtaaaa ccatcgtcgt
    15841 ttacggcact cgttaatccc gtcagagtgg cgcgttacct ggtagcgcgc cattttgttt
    15901 cccccgatgt ggcgcagact gatttatcac cccgatatca actatgcact tcgacaaacg
    15961 ctggtgctat gtttgcccgt ggccgttggg ttaatgcttg gcgaattacg attcggcctg
    16021 ctcttctccc tcgttcctgc ctgttgcaat attgcgggcc ttgatacgcc tcataaacgt
    16081 tttttcaaac gcttaatcat tggtgcgtcg ctgtttgcca cctgtagctt gctgacacag
    16141 gtgctactgg caaaagatgt tcccctgccc tttttgctga ccggattaac gctggtactt
    16201 ggcgtcactg ctgagctggg gccattgcac gcaaaattgc ttcccgcatc gctgctcgcc
    16261 gccattttta ccctcagtct ggcgggatac atgccggtct gggaaccgtt gctcatctat
    16321 gcgctgggca ctttgtggta cggattgttt aactggtttt ggttctggat ctggcgcgaa
    16381 caaccgctac gcgagtcact aagtctgctg taccgtgaac tggcagatta ctgtgaagcc
    16441 aaatacagcc tgcttaccca gcacaccgac cctgaaaaag cgctgccgcc gctgctggtg
    16501 cgccagcaaa aagcggtcga tttaattacc cagtgctatc agcaaatgca tatgctttcc
    16561 gcgcaaaata ataccgatta caagcggatg ctgcgtattt tccaggaggc gctggatttg
    16621 caggaacata tttcggtcag tttgcatcag ccggaagagg tgcaaaagct ggtcgagcgt
    16681 agccatgcgg aagaagttat ccgctggaat gcgcaaaccg tcgccgctcg cctgcgcgtg
    16741 ctggctgatg acattcttta ccatcgcctg ccaacgcgtt ttacgatgga aaagcaaatt
    16801 ggcgcactgg aaaaaatcgc ccgccaacac ccggataatc cggttgggca attctgctac
    16861 tggcatttca gccgcattgc tcgtgtgctg cgcacccaaa aaccgctcta tgcccgtgac
    16921 ttactggccg ataaacagcg gcgaatgcca ttacttccgg cgctgaaaag ttatctgtca
    16981 ctaaagtctc cagcgctacg caatgccgga cgactcagtg tgatgttaag cgttgccagc
    17041 ctgatgggca ccgcgcttca tctgccgaag tcgtactgga tcctgatgac agtattgctg
    17101 gtgacgcaaa atggctatgg cgcaacccgt ctgaggattg taaatcgctc cgtgggaacc
    17161 gtggtcgggt taatcattgc aggcgtgacg ctacacttta aaattcccga aggttacacc
    17221 ctgaccttga tgctgattac caccctcgcc agctacctga tattgcgcaa aaactacggc
    17281 tgggcgacgg tcggttttac tattaccgca gtgtataccc tgcaactgtt gtggctgaac
    17341 ggtgagcagt acatccttcc gcgtcttatc gataccatta ttggttgttt aattgctttc
    17401 ggcggtactg tctggctgtg gccgcagtgg cagagcgggt tattgcgtaa gaacgcccat
    17461 gacgctttag aagcctatca ggacgcgatt cgcttgattc tgagcgagta cccgcaacct
    17521 acgccactgg cctggcagcg aatgcgggtg aatcaggcgc ataacactct gtataactca
    17581 ttgaatcagg cgatgcagga accggcgttt aacagccatt atctggcaga tatgaaactg
    17641 tgggtaacgc acagccagtt tattgttgag catattaatg ccatgaccac gctggcgcgg
    17701 gaacaccggg cattgccacc ggaactggca caagagtatt tacagtcttg tgaaatcgcc
    17761 attcagcgtt gtcagcagcg actggagtat gacgaaccgg gtagttctgg cgatgccaat
    17821 atcatggatg cgccggagat gcagccgcac gaaggcgcgg caggtacgct ggagcagcat
    17881 ttacaacggg ttattggtca tctgaacacc atgcacacca tttcgtcgat ggcatggcgg
    17941 cagcgaccgc atcacgggat ttggctgagt cgcaagttgc gggattcgaa ggcgtaatac
    18001 aggctaaacc atcggatgag gtgttcacgc cgcatccgac atcttttgcc tgatgcgtct
    18061 tatttacgcc ccaatcacct tcgccaccgc gtgggcgaaa cgatgcatcc cttcatcgat
    18121 atccgcatct tccaccacca gcgacggcgc aaaacgcatc acatcaggcc cggcattcag
    18181 caccattacg ccttcctctg caccggcata caggaaatca cgcgcccgac ctttgtactg
    18241 tggtttcagc tctgcgccaa tcaacagccc cataccgcga atatcgctaa acacatcgta
    18301 ctgctgatcg atcttctgca aatggtcaac aaaacgctgg cgtttcgcct gaataccttc
    18361 cagcacttca ggggtattga tgatatcaaa cgccgcgccc gctactgcac aggccagagg
    18421 attaccgccg taggtggaac cgtgagaacc aggatgaaac gcggaagcaa tctccgccgt
    18481 ggtcagcatg gcgctaaccg ggaagccgcc acctagcgct ttcgcagagg tcagaatatc
    18541 cggcgtcacg ccgtagtgca tataagcaaa cagatcgccg gtccgcccca tcccgcactg
    18601 cacttcatca aacaccaata atgcctgatg ctgatcacac agctcgcgca agccctgcaa
    18661 aaactctggc gtcgctgccg tcacgccgcc ctcgccctga atcggctcaa ccaccaccgc
    18721 acaggtatgg tcatccatca cggcttttac tgcgtgaaga tcgttaaaag gcacgtggat
    18781 gatgtctgcc ggttttggcc caaagccgtc ggaatatttt ggctgcccac ccaccgaaac
    18841 ggtaaacagc gagcgaccat gaaaagcgtt atggaaggca ataattttgg ttttgaacgg
    18901 gctatgacgc acacaggcgt aatggcgtgc cagtttaaag gcggtttcgt tagcttccgt
    18961 gccggagttc ataaacacca cgcgttcggc aaacgttgcc tcaatcagtt tacggccaag
    19021 acgcagcgcc ggctcattgg tgaaaacgtt actaatatgc cacagagttt cgccctgggt
    19081 ttttaacgcg ttcaccagcg caggatggca atggcccaac gccgtaactg caatgccacc
    19141 cgcgaaatcg acatactcct tgccctgttg atcccagatt cggctgccct gaccttttac
    19201 cggaataaac tctgccggag cataaatcgg cagaatcact tcatcgaaag tcgcgcgtgt
    19261 aattgctgtt tgttcaattg ccatctcatg atcaccctgt tacgcataaa taaatatgaa
    19321 attataatca caaaatatgc ataaaaaatc actgaatggc aatcagaaat cagcgatgca
    19381 gaaaattagc cagcagttga tgtccttgtt cgctaagaat actttctgga tggaattgca
    19441 caccttccag atcccactgg cgatggcgaa tccccataat ctcgcgggtt tcgctccagg
    19501 ccgtcacttc aaagcactcc ggtaatgagt caggttccac caccagcgaa tggtagcgtg
    19561 tcacggtgag tggatttgcc agccccttaa atacgcccac accattatgt gtaatcggcg
    19621 aggttttgcc gtgcatgacc tttgcggcgc gcacaacttt gccgccaaat gcctgcgcca
    19681 ttgcctgatg accgaggcag acgccaagaa tcggcaagcg tccggcatag tgacgaataa
    19741 cgtcaaggga gatcccggct tcatctggcg tacaggggcc gggtgagatg acaatttttt
    19801 gtggtttaag ggcgtcgata tccgccaacg tcaacgcatc gttgcgctta accaacacat
    19861 ccgcccccag ttcacaaaag tactggtaga ggttccaggt aaaagaatcg tagttatcaa
    19921 taagcaggat catggcggct ccggtacgaa agaaccgcgc tattttactc agattccccg
    19981 gcttcgctta ccactttgct aaatatcatc tgcagcgcgg tgagatcccc cattgcccca
    20041 ttctgatttg cctgattcca ggcctctttt tcaataccct gccagctcag ttgatatccg
    20101 gcatgaatcg ccagttgctc gaagaagatc cgctgtgcca aaccacttcc cacccggaag
    20161 ggatgcagca cgttgatttc acaatagtaa tgcgccagcc gctcgacgaa cttcgctttc
    20221 tccaggccaa ccagataacc ttcttcctcc agatcctgca tcagggcatt gccctctttt
    20281 tcgatataag caaagtggca gaacggagta tcgccctgat aaatatcgac ttcgcgcagt
    20341 tgccccgccc agtcgaaaat atcctgatac aactggcgat gaagagtacg caaatgcggt
    20401 aaaccccgaa ccaacgggcc aagttcaatg gtcgcagcac gcagcgccgt catttcgtaa
    20461 gcggcctgtt ccagccgctg ctgctggcgg atgttcagcc ggttacgcat gatatcaagg
    20521 cctggataaa gatacggatc gcgcccttcg ccgaatttat cgctcatagt gcctcctcag
    20581 cgcttcaaga cgcgctaaag cctctgcggc agtaagagtg actaaaggca tctcgacgcc
    20641 ttcaagacgg cgactggcct ggaaattacg attacgctga agctcccaga gacgggactt
    20701 ttgcttatcg gtgagtttct tcacttgatg cctccctgaa cgtgtccatt tgccacaagt
    20761 ataagcggca aatccgggtt acgccgggag agcagaagcg cgggcaagat tgcccgcgcg
    20821 agaaatcatt acggcaggac tttagcggaa aggataacta ccggttttga cggcacattc
    20881 tggtacggac caacgtcatg agtcggcacc tgggaaatct tatcggcaac gtccatgcct
    20941 ttcaccactt taccaaatac cgcgtaaccg aaatcacgct gaccgtggtc aaggaaggcg
    21001 ttatcggcaa cgttgataaa gaactggctg gtggcgctgt ctttgtcagc ggtacgcgcc
    21061 atcgcgatgg tgccacgcgt gttgcgcagg ccgttatcag cttcattttt gattggcggg
    21121 tttggttttt tctgctgcat ctgctcggtg aaaccgccgc cctgaatcat aaagccagga
    21181 atgacgcggt gaaaggtcgt gttgttataa aaaccactgt tcacataatc gacaaagttt
    21241 tgcacagaca ctggtgcttt ttgtttatcc agctccagtt cgatgttacc agctgaggtt
    21301 gtcaacaata cgtgcgggtc ccctttcgct gccattgctg cgggagaaag agcagaaaga
    21361 gcgaaaacag cagccatcgc cgccagggtc gatttgaaca tgagatttcc ttaacaaaca
    21421 gagcagaaaa aaagcaagtg aagagattct aaatagcctt caagaacaag gccatccctt
    21481 tacctaattt tacgtatctg aaatatatgt aacctgagga taacttttta ttccgtgatc
    21541 aaaatcacct cttaaaatgc aatttagcaa ccgattgcaa taaaacattt aatcagatca
    21601 caaaatcgcc taaaatcgcc cgctcgcggc atagcccgct atgccatttt atttttgaac
    21661 gcaggccaat catgactaac agcaatcgca tcaagctcac atggattagc tttctttcct
    21721 acgcactgac cggtgcgttg gttattgtca ccgggatggt gatgggaaat atcgccgatt
    21781 atttcaatct gcctgtttcc agcatgagta acaccttcac cttcctcaat gccggcattt
    21841 taatctctat cttcctcaac gcctggctga tggaaatcgt cccgttgaaa acgcagttac
    21901 gttttggctt tctcctgatg gtgctggcgg ttgccggttt gatgttcagc cacagcctgg
    21961 cgctgttctc ggcggcgatg ttcattctcg gggtggtcag cggcatcacc atgtcgattg
    22021 gtacattcct ggtaacacaa atgtatgaag gacgtcagcg cggttcacgc ctgttattta
    22081 ccgactcctt cttcagtatg gccgggatga ttttcccaat gatcgccgcg tttctgctgg
    22141 cacgtagcat tgagtggtac tgggtttatg cctgcatcgg gctggtgtat gttgctattt
    22201 ttattctgac cttcggctgt gaattcccgg cactgggcaa acatgcgcca aaaacagatg
    22261 ctccggtaga gaaagaaaag tgggggatcg gcgtactgtt tctctctgtt gcggcgctgt
    22321 gctacatcct cggtcagtta ggttttatct cctgggtgcc agaatatgcc aaaggtctgg
    22381 gtatgagcct gaatgacgct ggcacgctgg taagtaactt ctggatgtcg tacatggtcg
    22441 gcatgtgggc gttcagcttt attcttcgct tctttgattt gcaacgtatc ctgaccgtac
    22501 tggctggtct ggctgcgatt ctgatgtacg tctttaacac cggaacaccg gcatatatgg
    22561 cgtggtcaat tctcgccctg ggcttcttct ccagcgcgat ctataccacc atcatcaccc
    22621 tgggttcaca gcagaccaaa gtaccgtcgc caaaactggt taactttgtc ctgacctgcg
    22681 ggactatcgg tactatgttg acctttgtgg ttaccggccc gattgttgaa catagtggtc
    22741 cgcaggcggc gctgctgacg gcaaacggtc tgtacgctgt cgtctttgtg atgtgcttcc
    22801 tgttaggttt cgtcagccgt caccgtcagc ataacaccct gacctctcat taattgttca
    22861 taccgggcgg cactatcgtc gtccggcctt ttcctctctt actctgctac gtacatctat
    22921 ttctataaat ccgttcaatt tgtctgtttt ttgcacaaac atgaaatgtc agacaattcc
    22981 gtgacttaag aaaatttata caaatcagcg atatacctct taaggagtat ataaaggtga
    23041 atttgattta catcaataag cggggttgct gaatcgttaa ggtaggcggt aatagaaaag
    23101 aaatcgaggc aaaaatgagc aaagtcagac tcgcaattat cggtaacggt atggtcggcc
    23161 atcgctttat cgaagatctt cttgataaat ctgatgcggc caactttgat attaccgttt
    23221 tctgtgaaga accgcgcatc gcttatgacc gcgtacacct ctcgtcttac ttctctcacc
    23281 acaccgccga agagctgtcg ctggtgcgcg aaggcttcta cgagaaacac ggcatcaaag
    23341 ttctggtcgg cgaacgcgct atcaccatca accgtcagga gaaggtgatt cactccagcg
    23401 ccggacgtac cgttttttat gacaagctga tcatggcgac cggttcctac ccgtggatcc
    23461 cgccaattaa aggttctgat actcaggact gctttgtcta tcgcaccatt gaagacctca
    23521 acgccattga atcctgcgct cgtcgcagca agcgcggtgc cgttgttggt ggcggcctgt
    23581 taggtctgga agctgctggc gcgctgaaaa acttaggcat tgaaacccac gttatcgaat
    23641 ttgcccctat gctgatggcg gaacagcttg atcagatggg cggcgagcag ctgcgtcgca
    23701 aaatcgaaag tatgggcgtg cgcgttcaca ccagcaaaaa cactcttgag attgtgcagg
    23761 aaggtgttga agcgcgtaaa accatgcgtt ttgccgacgg cagcgaactg gaagtcgact
    23821 ttatcgtctt ctctaccggt atccgtccgc gcgataagct ggcaacccag tgtggtctgg
    23881 acgttgctcc gcgtgggggt atcgtcataa atgattcctg ccagacttcc gatccggata
    23941 tctacgccat cggtgaatgc gcaagctgga acaaccgtgt atttggtctg gtagcaccag
    24001 gctacaaaat ggcgcaggtc gccgttgacc atattcttgg tagcgaaaac gcctttgaag
    24061 gtgcagacct tagcgccaag ctgaaactgc tgggcgtaga cgtaggcggt attggtgatg
    24121 cgcacggtcg cacgcctggc gcacgtagct acgtttacct cgacgaaagc aaagagatct
    24181 acaaacgcct gattgtcagc gaagacaaca aaaccctgct cggtgcggta ctggtgggcg
    24241 ataccagcga ctacggcaac ctgctgcaac tggtgctgaa cgctatcgag ctgccggaaa
    24301 acccggattc cctgatcctg ccagcgcact cgggtagcgg taagccgtct atcggtgttg
    24361 ataaactgcc ggacagcgcg caaatctgct cctgcttcga cgtcaccaaa ggcgacctga
    24421 ttgctgctat caacaaaggc tgccacaccg ttgcggcgct gaaagccgaa accaaagccg
    24481 gtacgggctg cggtggctgt atcccgctag tcactcaggt actgaacgcg gaactggcga
    24541 aacagggcat cgaagttaac aacaacctgt gcgaacactt tgcttattcg cgtcaggaac
    24601 tgttccattt gatccgcgtt gaaggcatta aaaccttcga agaactgctg gcgaaacacg
    24661 gcaaaggtta cggttgtgaa gtttgtaaac cgaccgtcgg ttcgctgctg gcctcctgct
    24721 ggaacgaata cattctgaag ccggaacata ctccgctgca ggattctaac gacaacttcc
    24781 tcgcgaacat ccagaaagac ggcacctact cggtgatccc gcgttctcca ggcggtgaaa
    24841 tcaccccgga agggctgatg gcagtgggtc gtatcgcgcg tgaatttaat ctctacacca
    24901 aaatcaccgg ctcccagcgc ctggcgatgt ttggcgcaca gaaagacgat ctgccagaga
    24961 tctggcgtca actgattgaa gccggcttcg aaaccggtca tgcctatgcg aaagcactgc
    25021 gtatggcgaa aacctgcgtg ggaagcacct ggtgccgcta cggtgttggc gacagcgtcg
    25081 gcctcggcgt ggaactggaa aaccgttaca aaggcatccg tacgccgcac aaaatgaagt
    25141 tcggtgtctc cggctgtacc cgtgaatgtt cagaagctca gggtaaagac gtgggtatta
    25201 tcgccaccga aaaaggctgg aacctgtacg tttgcggtaa cggcggcatg aaaccgcgtc
    25261 atgcggatct gctggcagcg gatatcgatc gcgaaacgct gatcaaatat ctcgaccgct
    25321 tcatgatgtt ctacatccgt actgccgaca aactgacgcg taccgcgccg tggttagaaa
    25381 acctcgaagg tggcatcgat tacctgaaag cggtgatcat tgacgacaaa ctggggctga
    25441 acgctcatct ggaagaagag atggcgcgcc tgcgggaagc ggtagtgtgc gaatggactg
    25501 aaacggtcaa tacgccgtct gcgcagactc gcttcaaaca cttcatcaac agcgacaagc
    25561 gtgacccgaa cgtgcagatg gtgccagagc gcgaacagca ccgtccggca acgccgtatg
    25621 aacgtatccc ggtaactctg gtggaggaca acgcatgagc cagtggaaag acatctgcaa
    25681 aatcgatgac atcctgcctg aaaccggcgt ctgcgcgctg ttaggtgacg aacaagtagc
    25741 aattttccgc ccgtatcaca gcgatcaggt gtttgcgatc agcaacatcg acccgttctt
    25801 cgagtccagc gtgctgtcac gcgggttgat tgcggaacac cagggcgagc tgtgggtcgc
    25861 cagtccgctg aaaaagcagc gttttcgctt aagcgacggc ttgtgcatgg aagacgaaca
    25921 gttttccgtc aagcattacg acgcgcgagt gaaagacggc gtggtacagc tgcgcggtta
    25981 atattttaac gggaggcgca atgcctcccc tttttgcatg gtcctgtaat aaccttcggt
    26041 atattgcagg acatttttta aactttttgt tttatttttt gtttttattt tttaaaggat
    26101 aatcaaatgt ttacagacac tattactaag tgtgcggcta acgctgcgcg cattgcacgc
    26161 ctgtcggcaa ataacccgct cggcttttgg gtcagctccg ccatggcggg cgcgtatgtg
    26221 ggtcttggga tcatcctgat tttcacgctc ggtaatttgc tcgacccgtc cgtacgtcct
    26281 ctggtgatgg gcgcgacctt tggtatcgcc ttaacgctgg tgattatcgc cggttctgaa
    26341 ttgttcaccg gacacaccat gttcctcacc tttggggtga aagcgggcac catcagccac
    26401 gggcaaatgt gggcaatcct gccgcaaacc tggctgggta acctggtcgg ttccgtcttc
    26461 gttgccatgc tttatagctg gggcggcggt agcctgctgc cggtagatac cagcatcgtt
    26521 cactccgtcg cgctggctaa aaccaccgca ccggcaatgg tattattctt caaaggcgca
    26581 ttgtgtaact ggctggtttg tctggctatc tggatggcat tgcgtacaga aggcgcggcg
    26641 aaatttatcg caatctggtg gtgtctgctg gcatttatcg cgtccggcta cgagcactcc
    26701 atcgctaaca tgacgctgtt cgcactctcc tggttcggca accacagcga agcctacacg
    26761 ctggcgggca ttggtcataa cctgctgtgg gtgacgctgg gtaatacttt atcgggtgcc
    26821 gtattcatgg gattgggtta ttggtatgct acgccgaaag cgaatcgtcc ggttgcggac
    26881 aaatttaatc aaactgaaac ggctgccggt taattattaa ggggttttta cgtggatcat
    26941 ttgcctatat tttgccaatt acgcgatcgc gactgtctga ttgtcggcgg tggtgatgtc
    27001 gcggaacgca aagcaaggtt gctgttagac gcaggcgctc gcttaacggt gaatgcatta
    27061 gcgtttattc cacagttcac cgcatgggca gatgcaggca tgttaaccct cgtcgaaggg
    27121 ccatttgatg aaagccttct cgacacctgc tggctggcga ttgcagcgac ggatgatgac
    27181 gcgcttaacc agcgcgtcag cgaagccgct gaagctcgtc gcatcttctg taacgtggtc
    27241 gatgcgccga aagccgccag ctttattatg ccgtcgatta ttgaccgctc accgctcatg
    27301 gtagcggtat cctctggcgg cacctctccg gttctggcgc gcctgttgcg cgaaaaactc
    27361 gaatcactgc tgccgttaca tctgggccag gtagcgaaat acgccgggca attacgcggg
    27421 cgagtaaaac agcagttcgc cacgatgggt gagcgtcgcc gtttctggga gaaattgttc
    27481 gttaacgacc gcctggcgca gtcgctggcc aacaacgatc agaaagccat tactgaaacc
    27541 accgaacagt taatcaatga accgctcgac catcgcggtg aagtggtgct ggttggtgca
    27601 ggtccgggcg atgccgggct gctgacactg aaaggtctgc aacaaattca gcaggcagat
    27661 gtggtggtct acgaccgtct ggtttctgac gatattatga atctggtacg ccgcgatgct
    27721 gatcgcgttt tcgtcggcaa acgcgcggga taccactgcg taccgcagga agagattaac
    27781 cagatcttgc tgcgggaagc gcaaaaaggt aaacgcgtgg tgcgtctgaa aggcggcgat
    27841 ccgtttattt tcgggcgtgg cggagaagag ctggaaacac tgtgcaacgc gggtattccg
    27901 ttctcggtgg ttccgggtat taccgcagct tctggttgct ctgcctattc gggtattccg
    27961 ctcacgcatc gcgattatgc ccagagcgta cgcttaatta ccggacactt aaaaaccggt
    28021 ggcgagctgg actgggaaaa cctggcggca gaaaaacaga cgctggtgtt ctatatgggg
    28081 ctgaatcagg ctgcaactat tcagcaaaag ctgattgaat acggtatgcc tggtgaaatg
    28141 ccggtggcaa ttgtcgaaaa cggaacggca gtcacgcagc gcgtgattga cggtacgctc
    28201 acgcagctgg gcgaactggc gcagcaaatg aacagtccat cgctaattat tattggtcgg
    28261 gttgttggcc tgcgcgataa attgaactgg ttctctaacc attaatttaa ccagggccag
    28321 agaagtctgg cctttttcgt ttacaactct ctttatatat tcagcagata aaaacataac
    28381 cccttaataa atattgtctg ctaatgggtt atattcaccg ttacagcgac ttacaaaatt
    28441 aagtcaatcg cttactgacg ctaattgaat attcctgtat cgttcttaat gagcagtctg
    28501 cttatattca tataatcaat gaatattaat taatatataa tacataggga atgtaaatga
    28561 acaaatttat taaagttgca ctggtaggcg cagtactggc tacgttaact gcatgtactg
    28621 gtcatattga aaaccgtgat aagaactgct cttacgacta cctgctgcac ccggctattt
    28681 ctatttctaa aatcattggc ggttgcggtc cgactgcaca gtaagtctct gacagaaatc
    28741 ggctaacgcc ggtttaattg cctgacgccc tcggagttat ccgagggcgt atttgtttct
    28801 gcattctttt caaaaatatc gtttacaacg attaaatgct ggcaatactt tcacgcaaga
    28861 tccgcaatat cgtctcttca ccgctgcgat ttgggttaat gcgaatcgcg caatgttcta
    28921 actgcggatt cgcctggcga aacgttccgg aaagtcgata aaagagcggc gggatttcat
    28981 attttgactc tgcaccaacc gggtaaggca attttgattg ctgcaaagat cgaaccgacc
    29041 agcaacgcca cttccatcag catattcatg gcgttacgga tgttatccga cgcattacgt
    29101 accgacgggt agcgccccaa ccatttgccg atcgaacgaa gcagcaagac ttccgccgaa
    29161 ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata gcccaccgca
    29221 taaacaaagg taaagcccgc aactgcatac acaccggtgg ctaacgcggt ggtggcaatc
    29281 aacggcacaa aacccagccc gcgcataaat tctgccagtg ccgcctggtt gattagtgtt
    29341 tgcgattgtt ccggcgttac gcctgcggag tacgctttct ccagtgtgaa gatcgacact
    29401 tcactaccgg cgaaaatctt catgctggca acggcagcaa tcaatgcacc cacgatggcg
    29461 atatacggta agtttttgat aatccgtgat gtacgctctt caaacaccga cataccgctg
    29521 gcatcgatat cattttcatc acgatgacgc aggtcatgag ttattgcgat ccccagcagc
    29581 atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggga atagcgcact
    29641 accaccacac gagtcatcag taccaccacg gcggcgatca gactttgctt ccagccaaac
    29701 tggtagaaaa tcgccaccaa cgggaacagt gcaaaagctg aaaccaccgg cgagcttaat
    29761 tcccccaggc tacctaatac atccaccggt agcgcggtca gcagctggtt tactggcaac
    29821 agacaggtga ggatcaatat tccccagata gcaccaagac caaacgccat caggctgttt
    29881 atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt gagcagcccg
    29941 gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag accaaaagcg
    30001 atactccccg cctcgcgacg gttcatatag ccttcaatca gttgtggcag gatcgggcgg
    30061 ataccgtcat gaaaaacggc cgccgagcga tgcgccagaa gcgatgtcat acccgtcagg
    30121 cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat ttcagatggt
    30181 taatcagcat cggaatcgcg tgttcaacgt gctcaacgga aaggccaaac gccacttttc
    30241 cttcagcgat cattttggcg atatgttcgt ctttcgcttt aatgcccggc ttggcaatgg
    30301 tgcaactttt gttatagcca atcaccgcga tagcaatcga caatgccgcg cccgcaccgg
    30361 tattacacgc accgatgtag taatccagtt gcccggattt cactttcatt gccgcttcca
    30421 tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg gtttttttaa
    30481 tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc ttattggaaa
    30541 aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa tcctgactgg
    30601 cgcaattgcg gaataaaggt ggtcaataaa aagtcatagc cataaccacc gttggctttt
    30661 aaatgagagc gccgcgtaat atccatcgac agcatgacgc ggttcagcaa cccacggtca
    30721 cgtagcgcat ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt cttgccgatg
    30781 gtgtcgaact gcacatacgc gccgagatcg atcatcttca aaatgttgtc gagattgtct
    30841 ttcagatcgc agtggccgac ggtgacgcgc gaaagatcaa ctccgtgggc ttgcagtagc
    30901 gccagttgtt ccagccccat ggtgctaaaa gaggtatgtg tggagatcgg gcatccggtc
    30961 tggttatgcg ccaacgcggc ggcaataaac accttttctt ccagcggcgt aatcttccct
    31021 tcgctggagc caatctccgc gatgatcccg gctttcagct ccgtgccatc gataccctgt
    31081 tcaatttcat cgaccatctc ctgcgccagt tgctctacgc tgcgggtcgc cacatgttcc
    31141 gggaaaaacg cgtcctggta ataaccggta caggccacca cgttgatccc ggtttcgcgt
    31201 attacatcga gcataaattg cgcattgcgc cccatataac ggttggtcat ctcaatcaca
    31261 ttacgcacgc cccgggccat caggtcgttc atctcctggc aaatgaaccc atattgatca
    31321 aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg ctcatgggcg
    31381 agcgtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg agggaggaaa
    31441 gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacact gtagccccaa
    31501 catcagaaag cgtggtgcga acaccaagct gagtagcagc caggccttgc tgataaacca
    31561 gcaccggcac cacttcgcgg gtatggtggc tgtgaccaat ggtcggatcg ttaccgtgat
    31621 ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagcctg gcaaggttac
    31681 ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga ccagcgaggt
    31741 cggtttcctg aatgttggtg caaataaacg ctgtcggatg cgtgttaaat tcgtcgaggg
    31801 tgatatccat aatcctctgg ctatccacca ggttttgcca gctcacgcca taaggattgc
    31861 tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca tacagttttt
    31921 gcggcacctg cactttttca tcaacaccat agcccatatg cacgacctgg aaaccgttgt
    31981 cataagcgcc agaacgcggc gcgttgatgc caataaagcg cccttctttg ctttctgcgg
    32041 catcgagaat gcgttgactg tcgggtaaca ggccgccaaa tgtaatgacc cgaccgacct
    32101 gtacctgctc acgcacaata cgacccattt tgattgcgtc gtcaaaagag atcacagaga
    32161 gattggcggt gatgttatag acctggccta aatccgcctc gagattatcg ccaatcgcaa
    32221 ccgcctgatt gacccacaga aattgcagat catcgccacg acgctccacc tgccaaccgg
    32281 cggatactaa tgcctgctca acacggtcaa tcacatcacg aaagggcatc cgcagcggcg
    32341 gtaatgggcg cgtacctaaa atctcctgat gccccataaa ggtatcgcca ccttcatgtt
    32401 gcagctctgc cacgccccag gttgcggagt ctgacggctg catatcgcca ggcgcataac
    32461 ccaatgcgtt gattagcccc agcgtctcca gcgttggtag ctgcaaatgc ggcaactggc
    32521 tcaggatgtg accacaggta ttcgccccgg catcctgcgg acgcaccaac gtgacatctt
    32581 tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc atttacgcct
    32641 ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgcca cggtgaatac
    32701 cggacaccag caccacatca ctacgggtga taaaaatctg cgtgcgaaaa cagagcacca
    32761 ctgcactgct taccggatgc tcgcctgcca gcggcagggt gtagtcgata ctgctgtcat
    32821 caacggcgtt gagatatgtt tcagtaattc tttgattttc tggcgtaaac accagcgcat
    32881 gttgcgcgtg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg ccacggaaat
    32941 gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg ccctgttgat
    33001 ttgccggaat agtacccgtc agcgcatgac cgggttcggt atgagtcaca ccgtattccg
    33061 ccagcaatgg cagcgacgtg cagctggtcg ctgaaggcgc gttcagttgc tcaatcgcaa
    33121 taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc ggtgtcggca
    33181 aaactttccc ggcagcctca tcccaaagca ggcaagggaa atgggtaagt cccgccaaat
    33241 gtagccccgg cagattctgg atttcagcaa ccacctcgtg cagcgaatgc tgtacaaaac
    33301 cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc agcacagact
    33361 gcacacgtcc ggcctccacc gctgccgcag aaacttcccg cgctttgtcg agagtaaaca
    33421 cggtgatgac gtcggttccc tgtgcaacgg cgtcaaccac ctgatgacaa gggatttgta
    33481 ccagatgccc ctgatgcgct accggcaaac aggcgcggcg catgactcgc gcctctttgt
    33541 aatccaccgc cacaatgccg ctgtagccca gagccaacag tttttctgcc agccacggat
    33601 tacgaccgaa ttgtttggtc atcagataca gttcaatgcc gtaaagccgc gccgtctcaa
    33661 tcagccgctt gccgttttcc agaacttgat ccacgtcgat cacccaactg tccggggcga
    33721 tcttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc tggcgtttca
    33781 atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata ttcatttttt
    33841 cgaatattta ggttgtgcga cgtggattta tcgggagctt caaacctctt cgttagcagc
    33901 catccataat ccataaaggt tggccagcaa atagccttct tcacacgggt taacttccag
    33961 tgcaaattcc ttcaacaaca cctgatgcaa ttgcacaacg gcttgccagt ggctggattg
    34021 cgccagttcc gccagcaatt cgtcatccag cggctctatt tcttcaccac ggcgactgcg
    34081 catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc gcaccggtag
    34141 atggcactct gtttccagta cattgacgac ctgcatcatg cctttgcaaa tgtccttatc
    34201 aataacgcct gcatcgcaaa gcaggttgag tctggtttcc atatcagatg cctttcgttt
    34261 aataactggg ttcctgttcc ccactgacta ctcgctgttg atgagcaagc agggcatgtt
    34321 tatcaacaac tgcatgcagc agttgttgca ttgggtaatc atcgactcgc gtcaggatca
    34381 cggcttcggt ggcctgtaaa aatcgcggat cgtctgtgag cggcgtcgct tctaatccca
    34441 gcatggtcag ttcgttttcc gccactacgt tccagataac cgcgtcaaca tcgcctttaa
    34501 caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca ctatcgccaa
    34561 aaaagacatc ggtcatgatt ttctgatctg ccgaacggtt atccagcccc acgcgcttca
    34621 cgtttgcgga ctcgccttta cggcaaatca actggtgctc gccaacgtag gtatgcggtc
    34681 ccaactccag cgcgaggcat aatccttttt gcgtgagata actttccgcc gccagtcgcg
    34741 aaaccaccgc catgtcatac acgccgttaa gtaaacactc cacgcgaata tccgcgccgc
    34801 gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg ctcgccaggc
    34861 cttcgtacaa acgggtatag ggcaagggca tcgcacatac cacgttgttg atatccacat
    34921 gagtcagcaa tgctttgtta tccatctcaa ccagataact gccattgcgc ccacggcgtt
    34981 caatccgtat cgctccgctt gattccagcg ttttcaacgc tgcctgcgtc aggccaaccg
    35041 atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg cacttttcac
    35101 ccaacaaata acgggccagt gtcgtgataa cgacgccttc ttttttgata aacgttcgac
    35161 gcataatata ttttcaataa aatgaatgtt tatatcttca ggaatttgaa gataaggcgc
    35221 aacagcgaga tgtggaaacg gcgaggcgct tcacattttt tcggattatt gcaaatgagg
    35281 ataaaaaaac cgggtttccc cggtttttgg cattaatgtt aaattacggc ttcgccacaa
    35341 aaccaatcgc ttcgtacacc gcttttagcg tacgggaagc gtgtgcgctg gctttttctg
    35401 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tggtaacgtt
    35461 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    35521 acatcttgcc ttcgaactgt ttttccagtt ccgggatgct ctggcccgtt accgcagaaa
    35581 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    35641 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    35701 gcaggccgat aacgttattg cggttatcgt cagacttgga cattttcttg gtcggctcca
    35761 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatat
    35821 cgccgtacaa cgcgttgaaa cgctgggcga tatcgcggct cagttccagg tgctgtttct
    35881 ggtcttcacc caccggcacc agattagttt gatacagcag gatgtccgca gccatcagca
    35941 ccggatagtc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    36001 actgcgtcat gcggctcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    36061 gcgcatgttc cggcacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    36121 aagccagata caacgccagc gtatccagcg tcgctttacg cagcttctgc gcatcctggc
    36181 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    36241 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    36301 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    36361 cctaatgcgg gcagaaggtc atttagagac tgataaatta catcaggctg gctgagatcg
    36421 atagcctcgc cgtagttata tccgtaggtt aagccaactg atggacaacc tgccgctttt
    36481 gccgcctgaa tatcattgcg tgagtcgccg acaaacagca tctgttgtgg agcaatgccc
    36541 atccgctcag ccaccagtag cagcgggtcc gggtgcggtt ttttattttg cacatcatca
    36601 ccaccaatca ccacgctgaa gtatttggcg atatctaagg cttcgagcag cggcgcgacg
    36661 aacggcgtcg gtttgttggt gaccaggcct agcggcaggc ctttagcctg caacgcgccc
    36721 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    36781 cgatcgaaca gtttacgcag gatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    36841 ggcggtttac ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    36901 cgctccatca gaatatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    36961 acaggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc gggtgcactg
    37021 tcaaccagcg taccgtcgag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    37081 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttgtagt ctggctggtc
    37141 gaagattgcc gaaccagcaa caaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    37201 gttcaccttc acgccaccgt ccacttccag tcgaatgtca aagccagact cgtcgatacg
    37261 acgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    37321 aggattgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    37381 cagaggtgtc gccgggttaa ataccaaacc cgctttacag ccattttctt taatcagttg
    37441 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    37501 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    37561 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    37621 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    37681 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    37741 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    37801 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    37861 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    37921 tgattggaga tcagcaccgg aatatggcga tcaaccagac cttcagcgat ctccgccaga
    37981 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcagtcgca
    38041 gacagcggcg cataaggcgg atcgcaatag acgacagatg catcatctgc gcgcgccatg
    38101 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    38161 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    38221 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    38281 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    38341 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    38401 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    38461 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    38521 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    38581 aaaaaagcgc gatttttctt catgctgact aactaattac accttctccg gctgtggaga
    38641 aagctccgac agcatccagc gcttcaggca acgcccgcaa tgataaacca ttgcgggaga
    38701 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    38761 ggcctggaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcata
    38821 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    38881 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    38941 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    39001 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    39061 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct ttgtgctcgc
    39121 cacaggggca gctggttcag ttcgcttcgg cgtctgtgcg actggcttcg gttccgtttt
    39181 cacggttgct tgcggttttt tcggttcaat caccgcctgc tgacgagccg ggcgcgtagt
    39241 ggccggacgt tcagcggttt gcgttttcgc cgtgtcacgc gatgcattgc cattgcgaac
    39301 aggcgcaacc gttgcgggtt cggtcggcaa tgtagaattg accgccacat tgttcagctg
    39361 ttgctgattt tgtggctggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg
    39421 ttgaccatcc gttgccgccg ggatttgccc ttgagtcggc gtagaagaga tcggcggcag
    39481 agaaacatcc tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt
    39541 ggcctgatcg gtcgcattgc ctgcaagatc aatactcttc tcgccagacg cggtttgatc
    39601 gctggaagag gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag
    39661 aaccagaatg ccgacaccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt
    39721 tttgcgctta cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc
    39781 gtcaagttct tcttcgattt ccggctcctc attacgcgct ttttgcgcac gagtcggacg
    39841 gcgatcgtca gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc
    39901 agtacgctca gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc
    39961 gggtttcagc tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat
    40021 gcttaccacg ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt
    40081 cttgttgtta cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc
    40141 cgctgcgaac ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa
    40201 ggactttctt gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc
    40261 cattgaccgg taacccagcc cgtgtgagca gggttataat acgctgcgtt tcggcagaac
    40321 taaactgccc gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt
    40381 caccatgtaa ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc
    40441 ccagattcag taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgctt
    40501 tcagctcaca acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg
    40561 catccagatt ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga
    40621 tgacttctgc cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat
    40681 ccaccaccac tgaagcgggc tggtagaacg cgccaatcat gtttttaccg aggggatggt
    40741 tgaccgcagt tttgccgcca acggaggagt cgacctgaga cagtaacgtc gtcgggactt
    40801 gaatgaaacg tacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcacccacta
    40861 cgccgccgcc aagcgccacc agagtagtat cgcgaccatg cggcttttgt aacaatgccg
    40921 taaagacggt attgagtaca gccaggcttt tatactgctc gccgtcaggg aggataacgc
    40981 tatcgacgtt aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag
    41041 ccagggtttc gttggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag
    41101 ctggttcatt aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa
    41161 cgacaatcct ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat
    41221 ataaagccag aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc
    41281 gctttgatca tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc
    41341 attggccaac gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt
    41401 tttatcacgc tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac
    41461 gccacgagcg gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc
    41521 cagcacaata ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa
    41581 gccttcttcg ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat
    41641 ctcttgatcg gaatcgtaaa attccatatt gagttgttga gctaactggc gcccaatagt
    41701 gctttttccg gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc
    41761 ggtactacta agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac
    41821 atgaactgaa acctcataag atattgcgag agtcagactg aaaattatct caatactcca
    41881 gcgggtttgg caactgaata aatcacccat ccctctgcat atctggtcgc tgcaagcgcg
    41941 ctgccttgct accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca
    42001 gcaaaatgcc gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa
    42061 ctctcaaatc ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg
    42121 ttcagcgtaa aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg
    42181 tgataaacac cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc
    42241 cgaaccaggg aatgtcgcca agcaacggta cgctatcctg acccgattta tttttacggg
    42301 taaaaatgcc acccagcgcc aacgtttctc cgcttttgac ctcgacctgc gtttcgatct
    42361 cctgcttatc aatcgccagc acttcgccat ctgcctgctg tagcacctgc cccggaacgt
    42421 tctggctgat gtgtaatttc agccggatgc gacctttttg taacaccgtg ggcgtgactt
    42481 ccatccccag gacggcctct ttaaattcta ccgacgtcgc gccactttcc ccgctggaaa
    42541 cctgatatgg aatttcgctc ccttgtttaa tgctggcagg ctgaagatgt gaggccagca
    42601 gacgcggact ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat
    42661 ccagtaaacg tccgttgatg cgtccaatgt taaaaccgat atgcgttgtc gccgtcgcta
    42721 cggagaggtc gctgccaagc gtagtgactt gcccaacgcc accaccttgt tgcgcatcgg
    42781 ccaacgtcca tttcacacct aactcacgca aacttttttc attaatggtg acaatatgcg
    42841 ccgacagctc aacctgcccg accggcagat ccatttgcga tacccactgt tcaagcgtgc
    42901 ttaacgccgt tttgttatct cgcagcaaaa ggcgattggt gcgtttatcg acggtcatac
    42961 tccctttggc actcagtagc ttctcccccg ctttcgccag ctctctggcg tcggcgtatt
    43021 gcagagtaat attgcgattt tccagcggca gatttgcctg cgcccgcgtc tgctccgcct
    43081 cctggcgggc gatattgtca ttctgccagg caacggaatg caccgagaga atgttgccct
    43141 cctggcgcgt tatcaatccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa
    43201 cgtcagttaa atgtaacgac accgtaccgc tgacgtctgg cgaaaccacc aggttcaact
    43261 tctcctgttc agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg
    43321 tcactttttg cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta
    43381 tccattgctt catttgtatc tccttgtcgt tgccacaacc agtgtggcgg ttcgcagttt
    43441 gtcccggttc ccagcgttag tgcgtctggc gtcagctgta agatcgtcca gccgttttcc
    43501 agcacatcgt tttgctgcac ccgtcgccat tttttttgcc catcttttat tacaccgatg
    43561 atgcgctcgc ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg
    43621 catagatctt cgggcggttt aaaagggtca cgcataccgg ttaaaaggca caatgcaatg
    43681 cctgctaaca accagcgttt aaccctcatg cggcgtctcc agttgtagcg tgaacaaaag
    43741 gtcatcacct tccacgctta acaaaaaacg gctcacgctg acgttgcgct ctgccagccg
    43801 tgtaaatgcc gacggcactg cttcccacag cgttttcaac gccaactcac ctccctgcgc
    43861 ggatggatgc cagtaaacca gttgcgcgcc ggataactga aaatccagtg gcgaaaaggg
    43921 cagcgttttt tcttcgctga aggaagtggt gtctaccagg cgatacagtg cagcccactg
    43981 ctggtgatga tttgcccgta gtcgaattaa tgcatcgctc tcttcatggt gtgtcaatga
    44041 cagaaaaatg agcgtaacta acaccagcaa ccagactgcc cagcaaaact ggcggaggcg
    44101 gagtgatgtg gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact
    44161 gatactcaaa ttgccagcgt ccctgcgcat cctgctgcgt ggcccccctc tgattgagat
    44221 gaaaagaagc atcctggcgg agtgacgttt ccagcgcgtt taacgcggca atgcttgttg
    44281 tcagcccctt gatctccagc gttccctgct gccagcttat cgttgtcagc caggcatgtt
    44341 cgggtaaaag cgccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct
    44401 ggcgctgcct ttgcaaacgc tgttcgcgta actgttggcg ttccagcaaa cgtggtttcg
    44461 ttatctgtaa gctgcgggcg agttgttgtt ccgcctgaag caaaacggcg tttacgcgtg
    44521 cttcggcatt gcttgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg
    44581 caacgaacat cagcaaccag aaacgaagaa aagcagtccg gcgttgctgt cgccagggca
    44641 aaaaattaat tggcgggttc atcagtacac cttcccaagc gccagcccca gcgcgatggc
    44701 aaagtctcca cctggtggcg gtagcggcgg ctgacgaaca gaaaccgcct cccaggggtc
    44761 aaatccgtct tcgccacata gcgcgacgct ttcgggatca acagataacg ctgccgccag
    44821 ctccttcgcg ctagtcatcc ccaccgccag tttgcgcccc cagctatagc gcgtcgccca
    44881 cagccactgt tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag
    44941 gaatcgctgt aatgcactgg catccggagt gatcgcgcta acatgaacac gcaacctttc
    45001 tgccagagta agcagcgttg ccagttcttt gctttgcgcg gcagtcacgt tataggcgag
    45061 actgagtgag tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagttcacg
    45121 ggccatcgtc ccggacagcc aggccatttg ctcccgctca ccgagggaca tcgacgggcg
    45181 tggaaatgac cgctgtaatg tgcgactggc gggaaacgct aacataatgt gatgacgctg
    45241 cggcagttca cgactccacg gtaacaacgt gttagccagc cgctgcacat caacaatccg
    45301 cccatctttg ataatgtcgt tctccagtgg caaccgccac cagcggtgca ataagcattc
    45361 ttttgcgctc cgcacgatag caaccgctac cgcttcttgc tgttgtaaat gcaaaccaat
    45421 ttgccagttc ttaaatgcca ttgtgatgat ctccttatca cccgtcactc tgacaggtat
    45481 atcaatgcgt ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga
    45541 aactaaatgg gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt
    45601 gcattctgct gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc
    45661 cggatgtggc gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg
    45721 atggcgagct gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa
    45781 tcccgccgga gatggtgaaa gcctttatcg cgacggaaga tagccgcttc tacgagcatc
    45841 acggcgttga cccggtgggg atcttccgtg cggcaagcgt ggcgctgttc tccggtcacg
    45901 cgtcacaagg ggcgagtacc attacccagc agctggcgag aaacttcttc ctcagtccag
    45961 aacgcacgct gatgcgcaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc
    46021 tgacgaaaga cgagatcctc gagctctatc tgaacaagat ttacctcggt taccgcgcct
    46081 atggtgtcgg tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgttga
    46141 acgaaatggc ggtgatagcc gggctgccga aagcgccttc caccttcaac ccgctctact
    46201 caatggatcg tgccgtcgcg cggcgtaacg tcgtgctgtc gcggatgctg gatgaaggat
    46261 atatcaccca acaacagttc gatcagacac gcactgaggc gattaacgct aactatcacg
    46321 cgccggagat tgctttctct gcgccatacc tgagcgaaat ggtgcgccag gagatgtata
    46381 accgttatgg cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca
    46441 aagtgcagca ggccgcacag caggcggtac gtaataacgt gctggactac gacatgcgcc
    46501 acggctatcg cggcccggca aatgtgctgt ggaaagtggg cgaatcggcg tgggacaaca
    46561 acaaaattac cgatacgctg aaggcgctgc cgacctatgg tccgctgctg cctgccgcag
    46621 tcaccagcgc caatcctcag gaagcaacgg cgatgctggc agacgggtcg accgtcgtat
    46681 tgagtatgga aggtgttcgc tgggcgcgcc cttaccgttc ggatactcag cagggaccga
    46741 cgccgcgtaa agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaagttg
    46801 gcgatgcatg gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc
    46861 cgcaaaacgg tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta
    46921 accgagccac tcaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg
    46981 cggcgatgga taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttcccgct
    47041 gggatgcagg tgccggttct gactggcaac cgaagaactc accaccgcaa tatgccggtc
    47101 caattcgctt acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgcgcaatgc
    47161 gggcgatggg cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcacaaa
    47221 acattgtcca caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg
    47281 cgcgcggcta cgcggtcatg gcgaacggcg gcttcctagt ggacccgtgg tttatcagca
    47341 aaattgaaaa cgatcagggc ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg
    47401 aatgcgatat tccggtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg
    47461 atgttgaaga tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc
    47521 tggagcaggc aaatcaggcg ttagtggcga agaccggcgc gcaggagtac gcaccgcacg
    47581 tcatcaacac tccgctggca ttcctgatta agagtgcctt gaacaccaat atttttggtg
    47641 aaccaggctg gcagggtact ggctggcgag caggtcgtga tttacaacgg catgatatag
    47701 gcggaaaaac gggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg
    47761 gcgttgtgac ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa
    47821 cggcttccgg agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc
    47881 agcctgcatg ggacgcttat atgaaagccg ttcttgaagg tgtgccggaa cagccgctga
    47941 cgccgccacc gggtattgtg acggtgaata tcgatcgcgg caccgggcaa ttagctaatg
    48001 gtggcaacag ccgcgaagag tatttcatcg aaggtacgca gccgacacaa ctggcggtgc
    48061 acgaggtggg aacgaccatt atcgataatg gcgaggcaca ggaattgttc tgattaaaaa
    48121 ggcgcttcgg cgcctttttc agtttgctga caaagtgcac ttgtttatgc cagatgcggg
    48181 tgaacgcgtt atccggccaa caaaaaattg aaaattcaat aaattgcagg aacttcgtaa
    48241 gcctgataag cgttgtgcat caggcaaact tcacgcattt acactcgccc ctgccctttc
    48301 aaccattcgc gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa gtcaggggct
    48361 tccagcaaat ccatcatatg cgccagcggc cagcgcacct gtggtaacgg ctctggctca
    48421 tcgccttcca gtgattccgg gtagagatct tgcgctacca cgatattcat tttgctggaa
    48481 aagtaagacg gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc accaaatcca
    48541 acctcttctt ttagctcgcg gttagcggct tcaaagacgc tttcacccgg atcaattaat
    48601 cctttcgaaa aacctaattc gtaggattca gttcccactg cgtattcgcg gatcaggatc
    48661 aggtgatcgt ccacaatcgg cacaatcatc actgcttccc ggttggttgg acgcattcgt
    48721 tcataaacac gccgcacgcc attgctgaac tccagatcca cgctttcgac ggtaaacagt
    48781 cgggaacggg ctacagtttc aacattcaga atggtgggtt tttgtaatga tttgctcatc
    48841 gtgggatcta tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc tttcggcaat
    48901 gtgaattgcg cgttatttac atttatgtaa cttaataaat aatcttcctc aaatcaaatt
    48961 aaaagtcaat aggttgaaat aactccagga atttgctgat attccgcctt cagagggttt
    49021 gctatgatca gcggttactg tgatgtgctt aatgatgctc aagttaaact ccacgcttgc
    49081 cgatagccaa ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc ctgacagact
    49141 aagtaagatg gggaaagcat gagcaccatt gtgatttttt tagctgcttt gctggcctgc
    49201 tcactacttg cgggatggct gataaaagtg cgatccagac ggcgtcagct gccctggacc
    49261 aacgccttcg cggatgcgca aacgcgtaaa ctcacatctg aagaacgtag cgccgttgaa
    49321 aattatcttg agagcctgac gcaggtatta caggtgcctg gcccaacggg agccagcgcg
    49381 gcaccgatct ctctggcgct gaatgccgaa agcaacaacg tcatgatgct gacacacgct
    49441 atcacgcgtt atggcatctc taccgacgat ccgaataaat ggcgttacta cctcgattcg
    49501 gtagaagtcc acctgccccc tttctgggaa cagtacatca acgatgagaa taccgttgaa
    49561 ctgattcata ccgattcgct gccgctggtt atttcactca acggtcatac gctgcaggag
    49621 tacatgcagg aaactcgcgg ctatgccttg caacctgttc cgtcaacgca ggcgtcgatt
    49681 cgcggggaag agagcgagca aatagagctg ctcaatattc gcaaagaaac gcacgaagag
    49741 tatgcgctga gtcgtccgcg cgggctgcgc gaagcgttgc tgattgtcgc ctccttcatg
    49801 atgttctttt tctgcctgat taccccggat gtctttgttc cgtggctggc aggcggcgcg
    49861 ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa atcctccctg
    49921 cgggaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg cgaaaacgat
    49981 caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc cgcacactgg
    50041 cagccataca ttgctcagga tctcggtcaa caaaccgata tcgatatcta tctcgaccgc
    50101 cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta
    50161 cagcactggc tgcgcagtac gattatcgct gcgggctcgc tactggtgct gtttatgctg
    50221 ttattctgga tcccgctgga tatgccgctg aaattcaccc tctcgtggat gaaaggcgca
    50281 cagaccattg aagccactag cgtaaaacaa ctggctgacg ctggcgtgcg ggttggcgat
    50341 acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggtac ctggagcgcg
    50401 aaaaccaatt cacctttttt accatttgac tgctcgcaga tcatctggaa cgacgcccgc
    50461 tcattgccat taccagaatc tgaactggtc aacaaagcga cggcattgac cgaagcagtt
    50521 aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc attacgttca
    50581 gcaattcaaa aatccggtat ggtgttgctt gatgattttg gcgacattgt tctgaagaca
    50641 gcagatctgt gttctgccaa agatgactgt gtgcgactga aaaatgcgct ggtcaatctc
    50701 ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa actcgatggc
    50761 gtgaatgtgt tattacgccc ggtgagtgcg gaatcgctgg ataacctggt ggccacctct
    50821 accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa cagtccggcc
    50881 cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca gccctggcct
    50941 tcggcatcac tttacgacta tccgccgcaa gaacagtgga acgctttcca gaaactggca
    51001 caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac
    51061 gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg cctgtggcgc
    51121 tatctcagaa ccacgctgct gctactgacg atgctgggta gcgccattta taatggcgta
    51181 caggcctggc gtcgttacca gcgccatcgt actcgcatga tgaagattca ggcctattat
    51241 gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga ataacacgtt
    51301 tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga tttccctcat
    51361 gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg acggcacgtt
    51421 gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa catggggcgc
    51481 gaaaaacgga gttacgccac aggaagcgat ggaatatatg cgccagcaat atcacgacgt
    51541 acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg gtctggatat
    51601 ctgtgcgatg accaccgaga tgggaccgcg tgccgtactg cgtgaagata ccattccgtt
    51661 tcttgaggca ctgaaagcca gcggtaaaca gcgaattttg ctcaccaatg cgcatccgca
    51721 caacctggcg gtaaaacttg agcataccgg tctggacgca caccttgatt tattactttc
    51781 cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg tggccgaagc
    51841 tacgggtctt aaagctgaaa gaacgctgtt tattgatgac agcgaagcga ttctcgatgc
    51901 tgccgcgcaa tttggtattc gttactgcct cggtgtgact aatcctgact ccgggattgc
    51961 cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga tcccctcgct
    52021 aatgtgaagg agatgccatg aaagaaaaac ctgctgttaa ggttcgactg gataaatggc
    52081 tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta
    52141 aggtgcatta caacgggcag cgcagcaagc caagcaaaat cgtcgagctg aatgccacgc
    52201 tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt actgaacagc
    52261 gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt gtagagaaac
    52321 gcgaaaaaat ggcgctggca cgtaaactta atgccttaac catgccgcat ccggaccgac
    52381 gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac agtgaataac
    52441 tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc tatctgtttg
    52501 aaaactttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa cagatccttg
    52561 agaatcacga ttatccgcag cccgttaaga acgtgctggc agaactgctg gttgcgacca
    52621 gcctgttaac cgctacgttg aagtttgatg gtgatatcac cgtacagctg caaggcgacg
    52681 gtccgatgaa tctggcggtt attaacggta acaacaacca gcagatgcgc ggtgtggcgc
    52741 gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc aatggttacg
    52801 tggtgatcac catcaccccg agcgaaggcg aacgctatca gggcgtggtt ggtctggaag
    52861 gtgataccct ggcggcctgc ctggaagatt actttatgcg ttctgaacag ctgccgacgc
    52921 gcctgtttat tcgcaccggc gacgtagacg gcaaaccggc tgcaggcgga atgttgttgc
    52981 aggtaatgcc tgcgcaaaat gcccagcagg acgactttga ccacctggcg acgctaaccg
    53041 aaaccatcaa aaccgaagaa ctgctgactt taccggcaaa cgaagtgttg tggcgtttgt
    53101 atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct
    53161 cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtc gatagcatcc
    53221 tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac tatctgttca
    53281 atgcgatgga tattgccgag atccgcaaca acgcgtctcc ggcagatccg caagttcatt
    53341 aatgagtatg tccggcagag aggactctgc cggattttac gactaataca cctgatgcgc
    53401 tttgtcactc attcagattc acatccagcc tttcaccatc cactacaaga tcgcgccacg
    53461 ctttaggcct cgtccccagc tttccatcct tcgcagctgt gagtaatttt tctttcgtta
    53521 ttttgcgcgg aagtaaactc atatcaagcg cgtcccattc ttttctggtt gagtcaaagc
    53581 catagacaat gactctgtca tcattaaaag caaacaggat ccgctccgcc tgaccatcac
    53641 tattcagatc ttgctcgacc agtacgcagg cgtcttttcc aatacaggtc atcacgttat
    53701 agcgatcctg gattaaggcc gaccagaatg tcgcatccgg tttaccagaa cctggggcaa
    53761 ttaacacatt atcggctaat actttttccg atacctgttc ctgaagatgt tgctctccat
    53821 ctaacgccat cagcagatcc cgcgcgcgtt tcgggtcttt catatactcg gcatcgcttt
    53881 tcagcgactc aagcgccgca cgtccatagc gaccgctctg ttcgagcatg tagatagtta
    53941 cctggtctgg cgtgtttttg ccgctctgat aacgcgccat atgactgttc acgctaatgc
    54001 gcatactgtc cagcaccggc gaattaagaa gcaccagtat taccaacacc aataacgaaa
    54061 ccacaaggtt cactttgccc tgaagatcaa ggggattttg ccctttacgc cagacgatac
    54121 tgacaaaata ccccagcgac cataccagta acaccagcac tgccagcgcg ccctgcaagc
    54181 ggtcggcagt ccagccgtac tgagcgaccc gcagccataa cgcccaggcg gcgacgaaca
    54241 catacagcgg agcaaccagc aaagcggttt taatcaggca acgcaatggc cccgtccagg
    54301 gaagtgacgc tttttgcgga tcgcggacaa tagccattaa gatcaattgc aaaaaggcca
    54361 gcgttaacag caacccagcg gcggaaatgt ggcgagaaat cgcgctcagg cccgtaaacg
    54421 gcagggtgat aataaacatc agggttagca atgatactaa cggcagcaac cccgtggcga
    54481 ttaacgtgaa caacttttga atagagtcga ttaaacgtga ctgtgtacgc gcgaggatca
    54541 ccgccagtgc ggtaaccaga cctaacgtga gataaataaa cccgtcggtt gcaaaaaaga
    54601 gcgttttgaa aaacgtgatg ccaacaagtt taaacaactc tccccacagt aaaagtacca
    54661 gccacgtcag gccattggcg agaaaaatca ccagtaatat aagtaagtta tgccatactg
    54721 actggtaaaa atagcgatag cggctactgt cattgcggat gcgaaggctt tgttgtatcc
    54781 acggtagcaa caacattgcc atcagcagca gatagcagcc aaaatcccaa agcgcctttt
    54841 cagctctcca gggggtcatg ccatcagttt gccacttcag ccagtcgctc attcccagcg
    54901 tggcaataaa caccagcgcc agccatcccc aaaggcgatt ctgtttaaag gagatcacgg
    54961 agaagagtaa aacggatgaa aacgcgacgg tcgcaggcac gccataaaca atccaactga
    55021 gattttttcc ggcaagccag gcgatcagca ggtagcaaac caacccctga agtaatccgg
    55081 tagcaatcat cccccagcgt gttgccggtg aaagttcaac gttatccatg ttgtttatcc
    55141 agaatcaaaa ggtgggttaa ttatcgcatc cgggcagtag tattttgctt ttttcaaaaa
    55201 ataatcaaaa aaagttagcg tggtgaatcg atactttacc gattgaattt gcatcaattt
    55261 cattcatgaa tgcgattcca ctcacaatat tctcgccata taaaccaaga tttaaccttt
    55321 tgagaacatt ttccacacct aaaatgctat ttctgcgata atagcaaccg tttcgtgaca
    55381 ggaatcacgg agttttttgt caaatatgaa tttctccaga tacgtaaatc tatgagcctt
    55441 gtcgcggtta acacccccaa aaagacttta ctattcaggc aatacatatt ggctaaggag
    55501 cagtgaaatg cgcgttaaca atggtttgac cccgcaagaa ctcgaggctt atggtatcag
    55561 tgacgtacat gatatcgttt acaacccaag ctacgatctg ctgtatcagg aagagctgga
    55621 cccgagcctg accggttatg agcgcggggt gttaactaat ctgggtgccg ttgccgtcga
    55681 taccgggatc ttcaccggtc gttcaccgaa agataagtat atcgttcgtg acgataccac
    55741 tcgcgatact ttctggtggg cagacaaagg caaaggtaag aacgacaaca aacctctctc
    55801 tccggaaacc tggcagcatc tgaaaggcct ggtgaccagg cagctttccg gcaaacgtct
    55861 gttcgttgtc gacgccttct gtggtgcgaa cccggatact cgtctttccg tccgtttcat
    55921 caccgaagtg gcctggcagg cgcattttgt caaaaacatg tttattcgcc cgagcgatga
    55981 agagctggca ggtttcaaac cagactttat cattatgaac ggcgcgaagt gcactaaccc
    56041 gcagtggaaa gaacagggtc tgaactccga aaacttcgtg gcgtttaacc tgaccgagcg
    56101 catgcagctg attggcggca cctggtacgg cggcgaaatg aagaaaggga tgttctcgat
    56161 gatgaactac ctgctgccgc tgaaaggtat cgcttctatg cactgctccg ccaacgttgg
    56221 cgagaaaggc gatgttgcgg tgttcttcgg cctttccggc accggtaaaa ccaccctttc
    56281 caccgacccg aaacgccgcc tgattggcga tgacgagcac ggctgggatg atgacggtgt
    56341 gtttaacttc gaaggcggct gctacgcgaa aactatcaag ctgtcgaaag aagcggaacc
    56401 tgaaatctac aacgctatcc gtcgcgatgc gctgctggaa aacgtcaccg tgcgtgaaga
    56461 tggcacaatc gactttgatg atagttcaaa aaccgagaac acccgcgttt cttatccgat
    56521 ctatcacatc gataacatcg ttaagccggt ttccaaagcg ggccacgcaa ctaaagttat
    56581 cttcctgaca gcagacgcct ttggtgtgct gccgccggtt tctcgtctga ctgccgatca
    56641 aacccagtac cacttcctct ctggcttcac cgccaaactg gccgggactg agcgtggcat
    56701 caccgaaccg acgccaacct tctccgcctg cttcggcgcg gcgttcctgt cgctgcaccc
    56761 aactcagtac gcagaagtgc tggtaaaacg tatgcaggcg gcgggtgcgc aggcgtatct
    56821 ggttaacacc ggctggaacg gcactggcaa acgtatctcc attaaagata cccgcgctat
    56881 tatcgacgcc atcctcaacg gttcgctaga taacgcagaa accttcactc tgccgatgtt
    56941 taacctggcg atcccaaccg aactgccggg cgtagacacg aagatcctcg atccgcgtaa
    57001 cacctacgct tctccggaac agtggcagga aaaagccgaa acgctggcga aactgtttat
    57061 cgacaacttc gataaataca ccgacacccc tgcgggtgcc gcgctggtag cggctggtcc
    57121 gaagctgtaa tcagctgata tctcattgac cccttatcca aacggataag gggttttttt
    57181 atatctaatt agctaccaat atgtacactt ataattatca atagaccgca ggatgcatta
    57241 tgttcacctt tattgaacta caaggattta gcaaacgccg ccagttattg ctacctgata
    57301 agtagttccg ggcttttcag gaactactca ttcaagatcc cactgccggt gacattatcg
    57361 caggcacagg cagttttaga aaaattcgct ggaaccgttc tggaatgggg aaacggggtg
    57421 gcattcgaat aatttattac aacgtaacaa gcaatggtcg aatttatctg gcgttgattt
    57481 atcctaaaaa tgaacaggat gatttaacag aagaacaaag aaaggcattg aaactattgt
    57541 ccgagaagct gctttaatgt tgaagctcca caaattccac catggtcgaa atgccaacgg
    57601 agacattgaa aacagatgaa agatgaatta tttgctgatc ttcttgccag cgcagaagaa
    57661 atggtacgca tcgaaaaggg tgaagaaacg cctgaaccag agcacgtcca tacttttagc
    57721 gaaatcgatg taaaagcgat ccgtgaagca acgggtttac gtcagcaaga ttttgctgtc
    57781 gccgtagggg taagttatga cctggtcaaa agccgggaaa ccaaacgccg tcagcctacc
    57841 ggagcacccc gaaaattgct cctgcttttg caagcgaatc ctttcattat caatcaattg
    57901 aaaacaatct aaaaacggga ggcaccttca cctcccgttt ttttaccctt cttttgtcat
    57961 gccctgcgcc cgcgttaccg gcactggcag ccaggcgcga atggaaagcc cgccccgttc
    58021 gctggtgcca agctccagca tcccgttatg gttatccacg atacgctgca caatcgccag
    58081 ccctaatccc gtgccgctaa tggtgcgcgc actgtcacca cgaacaaacg gctggaacag
    58141 gtgcttacgt tgttccggcg caatacccgg accgtcatct tctacctgga accaggcgcg
    58201 attcggctcc gtgccgctgc tgactttgat ccagccattg ccgtaacggg cggcgttgac
    58261 caccatattc gccaccgcgc gtttgatcga cagcgggtgc attttcacct caatgctacc
    58321 ggggtaaagc gcagtttcaa tttcccgctc atagccactt tcggcagcaa taacctcacc
    58381 gagtactgcg ttgagatccg ccatttccat cggcatctcc tgcccggtgc gcaggtagtc
    58441 gataaactgc tcaatgatgg cgttgcactc ttcgatatct ttattgatcg attctgccag
    58501 gtagccatcc tgctcgctca tcatctcggt cgccagacga atacgcgtca gcggcgtgcg
    58561 cagatcatgg cttacccccg ccatcagcag cgtgcggtca tccgccagtt gcttaacacc
    58621 agccgccata tggttaaagg cgcgggtaac ggaacgcacc tccgaagcgc catattcacg
    58681 cagcggcggc ggaataatcc ctttacccac ctgcaaggct gcgtgttcga gatcgaccaa
    58741 cggtcggttc tggatacgaa taaacagcca cgccccgcct atcgccaata gcataatcgc
    58801 caacgtataa cggaacagcg gggagaaatc gccctgatga atttcggtta gcggcacgcg
    58861 tacccagata ttgggcgaca accaggtttt cagccagacg acaggcgaac ttttgttgac
    58921 ctcgacgcga acttccgtcg gaccgcccag ttgctgcgcc atctgatggc ttaagaattc
    58981 atagtgttgc gcccaacgca gacctgcctc ttcggcagct tcgttggaat agagagagat
    59041 ccccagctca cggtagatct cccgacggaa agcgggaggc acaaccaact gcgtgccgtc
    59101 ctccagttgc agtttgtcgg tcatcaacat acgcacttca tacgcgagga ctttattaaa
    59161 ctgctggagg ctcggcaaaa tcgcgaagtt cagcaccacc agataagtcg tcaccaggct
    59221 gacgaacagc aaggtgacga tgagcaataa cgtacgggca aatgaacttc gtggcgagaa
    59281 gcgcaatcgc ctcatgcttt agaaccgtcc ggtacaaaga cgtagcccag accccagacg
    59341 gtctgaatgt aacgcggatg cgccggatct tcttccacca tgcggcgcag acgcgaaatc
    59401 tgcacgtcga tggagcgttc cattgcggaa tattcacgac cacgggcaag gttcatcagc
    59461 ttatcgcggg agagcggctc acgcggatgg ctgaccagtg ccttcagtac cgcaaactca
    59521 ccactggtga gcggcatcgg ctcgtcttcg cggaacattt cgcgcgtacc gaggttaagt
    59581 ttgaacttac cgaaagcaat taccgcctct tcctgtgacg gtgcgcctgg cagttcgttc
    59641 gcctgacgac gcagcaccgc acgaatgcgg gccagcagtt cacgcgggtt aaacggtttt
    59701 ggaatgtaat cgtcagcgcc aatctccagg ccaacgatac ggtccacttc ttcccctttc
    59761 gccgtcacca taatgatcgg catcggattg ctctggctac gaagacgtcg gcaaatcgac
    59821 aagccatctt caccaggtaa catcaaatcc agtaccataa gatggaaaga ttcacgagtc
    59881 agcaggcgat ccatctgttc tgcattagcg acgcttcgaa cctggaagcc ttgttcggtg
    59941 agataacgtt ccagcagcgc gcgcaggcgc atgtcgtcat caaccaccag aatcttgtag
    60001 ttctcttgca ttgtttgtac tcccaaaggt tcgcaacaat ttgtaagcgt gtattcttaa
    60061 aaaagctcac gttcgtcacc agctaaatct ggtatgaatt tcagcctaaa ttgttacaaa
    60121 gcatattaaa cagcagctta agtatacaat ttattcggcg aaacattatt gattctgttg
    60181 atatgatcac gttataccca atgtgcgcat tatcaaacag acaaagggaa tcaacgagat
    60241 gaaaacgccc ctggttaccc gggaagggta tgaaaaactc aaacaagagc ttaattatct
    60301 ctggcgtgaa gaacgcccgg aggtcacaaa gaaagtgacc tgggccgcaa gtctgggcga
    60361 ccgcagcgaa aatgctgact atcagtataa taaaaagcgc ctgcgtgaaa tcgaccgtcg
    60421 cgtgcgctat ctcactaaat gcatggaaaa tctcaaaatc gtcgattact ccccgcagca
    60481 ggaaggcaaa gtcttttttg gcgcgtgggt ggagattgaa aacgacgatg gcgtgactca
    60541 ccgtttccgt attgtcggct acgatgaaat ttttggccgt aaagattaca tctctatcga
    60601 ttccccgatg gcccgcgcat tgctgaaaaa agaagtcggc gatctggcgg tggtgaatac
    60661 ccctgccggg gaagcgagct ggtatgttaa tgctatcgag tacgtgaaac cgtaaggaag
    60721 agtcttaacc tcctgccgat ggctggcatt tttgccagcc agtccgtata actatcccct
    60781 gatttttgat ccgaaaagat gaactcaaac catgatgaat gattcgttct gccgcattat
    60841 tgcgggtgaa attcaggcgc gcccggaaca ggttgacgct gccgttcgcc tgcttgacga
    60901 agggaatacc gtgccgttta tcgcacgtta tcgtaaggaa atcaccggcg gtctggatga
    60961 cacgcagctg cgtaatctgg aaacgcgtct gagctatctg cgcgagctgg aagagagacg
    61021 tcaggcgatc ctcaagtcca tttccgagca aggcaaactc accgatgatc tggcgaatgc
    61081 catcaacgcc accctaagca aaaccgaact cgaagacctc tacctgccct acaaacctaa
    61141 acgccgcacc cgtgggcaaa tcgccattga agcagggctt gagccgttgg ccgatctgct
    61201 gtggagcgat ccgtcacaca cgccagaagt cgccgctgca caatatattg atgccgataa
    61261 aggcgtggca gataccaaag ccgcgctgga cggcgcgcgc tatatcctga tggaacgttt
    61321 tgccgaagat gctgcgctgc tggcgaaagt gcgtgattat ctgtggaaga acgcgcatct
    61381 ggtctcaacc gtggtgagcg gtaaagaaga ggaaggggca aaattccgcg actatttcga
    61441 tcatcacgaa ccactgtcaa cggtgccttc tcaccgcgca ctggcgatgt tccgtgggcg
    61501 taacgaaggc gtacttcagc tttcgctgaa tgccgatccg cagttcgatg agccgccaaa
    61561 agagagctat tgcgagcaaa tcatcatgga tcatctcggc ctgcgcctga acaatgcccc
    61621 ggcggatagc tggcgcaaag gcgtggtgag ctggacgtgg cgcatcaagg tgctgatgca
    61681 tctggaaacc gaactgatgg gcaccgtgcg cgaacgcgcg gaagatgaag caatcaacgt
    61741 ctttgcccgt aacctgcacg atctgctgat ggcggcccca gccggactac gtgcaacgat
    61801 gggcctcgat ccgggtctgc gtaccggggt aaaagtagcg gtggtcgatg caactggcaa
    61861 actggtggcg accgatacca tttatccgca taccgggcag gccgcaaaag cagcgatgac
    61921 cgttgctgcg ctgtgtgaaa agcataacgt tgaactggtg gcgattggca acggtacagc
    61981 ttcccgcgaa accgaacgtt tctatctcga cgtgcagaag cagttcccga aagtgaccgc
    62041 gcagaaagtg atcgtcagcg aagctggcgc gtcggtttac tcggcttccg agctggcagc
    62101 acaggaattc ccggatctcg acgtttcgct gcgtggcgcg gtgtctatcg cccgtcgttt
    62161 gcaggatccg ctggcggagc tggtgaaaat cgatccgaaa tctatcggcg taggtcagta
    62221 tcagcatgac gtcagccaga cgcagctggc acgcaaactg gatgcagtag tagaagactg
    62281 cgtaaacgcc gttggcgtcg atctcaacac cgcttctgtt cctctgttaa cccgcgtggc
    62341 gggcctgacg cgcatgatgg cgcaaaacat cgtggcctgg cgcgatgaga acggccagtt
    62401 ccagaaccgt cagcaactgt taaaagtgag ccgtctgggg ccgaaagcct tcgagcagtg
    62461 cgcgggcttc ttgcgcatta accacggtga taacccgctg gacgcttcta ccgttcaccc
    62521 ggaagcctat ccggtggtgg aacgcattct ggcagcaaca cagcaggcac tgaaagatct
    62581 gatgggtaac agcagcgaac tgcgtaacct gaaagcatct gactttactg atgagaaatt
    62641 cggtgtgccg acggtaaccg acatcatcaa agagctggaa aaaccgggtc gcgatccgcg
    62701 tccggaattt aaaaccgctc agtttgccga tggcgtcgag acaatgaacg acctgcaacc
    62761 gggtatgatc ctcgaaggtg cggtgaccaa cgtcaccaac tttggcgcgt ttgtcgatat
    62821 tggtgtgcat caggacggcc tggttcacat ctcttcattg tcgaacaagt ttgtggaaga
    62881 tccgcatacc gtggtgaaag cgggcgacat tgtgaaggtg aaagtgctgg aagtggatct
    62941 tcagcgtaaa cgtatcgccc tgaccatgcg tctggacgag cagcctggcg aaaccaacgc
    63001 ccgtcgcggt ggcggtaacg atcgcccgca aaacaaccgc ccggcagcca aaccgcgtgg
    63061 gcgtgaagcg cagcctgccg gtaatagcgc gatgatggat gcgctggcgg cggcaatggg
    63121 caaaaaacgt taaacgcccg gactggcccc taaacctgcg cgattaggcg ttcacgccac
    63181 atctggcaac gaacgccttg cctgacataa aagtgccgga gaatatctcc ggccttttta
    63241 ttccacaagc aaactcataa tatatcccgt taatatttat cacttcatta acaactgaaa
    63301 ccttaattaa acattaacca gccctaataa ttcgtcatgc gaattatatt ttcgctgcga
    63361 tataaccttg agccatatca acattgagtc agattattat tcaaaccaac attcacacac
    63421 attttaagta ttgctgatag aaaccattct cattatcatt gtactgttga ttatttaatc
    63481 tctccttcgt tggcaaatca tctggtctca tgtcgctgtc aaacgcccca tgaggtagtt
    63541 atccagttaa tgagaaacaa gtaggcacct atgcaataca ctccagatac tgcgtggaaa
    63601 attactggct tttcccgtga aatcagcccg gcatatcgcc aaaaactgct ttctcttggc
    63661 atgttacctg gctcctcttt taatgtggtg cgcgtcgctc cactcggcga ccccattcat
    63721 atcgaaaccc gtcgtgtgag cctggtatta cgcaaaaaag atctggcctt attagaagtg
    63781 gaagcggttt cctgttaata cggtgataac aacaatgaaa aaattaacca ttggcttaat
    63841 tggtaatcca aattctggca agacaacgtt atttaaccag ctcactggct cacgtcagcg
    63901 tgtaggtaac tgggctggcg ttaccgtcga acgtaaagaa gggcaattct ccaccaccga
    63961 tcatcaggtc acgctggtgg acctgcccgg cacctattct ctgaccacca tctcatcgca
    64021 gacctcgctc gatgagcaaa tcgcctgtca ctacattttg agtggcgacg cagacctgct
    64081 gattaacgtg gtggatgcgt ctaaccttga gcgtaacctg tacctgacgc tgcaactgtt
    64141 ggaactcggc attccatgca tcgtggcgct gaacatgctc gacattgccg agaagcaaaa
    64201 tattcgcatt gaaattgatg cgctgtcggc gcgtcttggc tgcccggtga tcccgctggt
    64261 ttcaacccgt ggtcgcggta ttgaagcgct caagctggcg attgatcgct ataaagctaa
    64321 cgaaaatgtg gaactggtgc attacgctca gccgctgctc aacgaagcag attcactggc
    64381 aaaagtgatg ccttccgaca tcccgctgaa acaacgtcgc tggctgggcc tgcaaatgct
    64441 ggaaggcgat atctacagcc gcgcctacgc cggtgaagcg tcgcagcatc tggatgccgc
    64501 cctcgcccgt ctgcgtaatg agatggacga tccggcgctg cacattgccg atgcgcgtta
    64561 ccagtgcatt gctgccatct gtgatgtggt aagcaacacc ctgacggcag aacccagccg
    64621 tttcaccact gcggtagata aaatcgtgct caaccgtttc ctcggtctgc cgattttcct
    64681 ctttgtgatg tacctgatgt tcttgctggc tatcaacatc ggcggggcgt tacagccgct
    64741 gtttgacgtc ggctccgtag cactctttgt gcatggtatt cagtggattg gctacacgct
    64801 ccacttcccg gactggctga ctatcttcct cgcccaaggc ctgggcggcg gtattaacac
    64861 cgtgctgcca ctggtgccgc agatcggcat gatgtacctg ttcctctcct tccttgagga
    64921 ctccggctat atggcgcgtg cggcgtttgt gatggaccgt ctgatgcagg cgctgggctt
    64981 gccggggaaa tcctttgtac cgctgatcgt tggtttcggt tgtaacgtac cgtcggtaat
    65041 gggcgcacgt acgcttgatg caccgcgtga acgtctgatg accatcatga tggcaccgtt
    65101 tatgtcctgc ggcgcgcgtc tggctatctt cgcagtattc gcggctgcct tcttcgggca
    65161 gaacggtgcg ctggcggtct tctcgctgta tatgctgggt atcgtaatgg cggtgctgac
    65221 gggcctgatg ctcaagtaca ccatcatgcg cggtgaagcg acgccgtttg tcatggagct
    65281 gccggtctac catgtaccac acgttaaaag cctggttatc cagacttggc agcgtctgaa
    65341 aggcttcgtt ctgcgtgctg gtaaagtgat tatcattgtc agcattttcc tgagcgcctt
    65401 caacagcttc tcgctgagcg gaaaaatcgt cgataacatc aacgactcgg cgctggcttc
    65461 cgtcagccgg gtgatcaccc cggtcttcaa gccgattggc gtacatgaag ataactggca
    65521 ggcaacggtt ggcctgttta caggtgccat ggcgaaagaa gtggtggtgg gtacgctcaa
    65581 caccctctac accgcagaaa atattcagga cgaagagttc aatccggcgg aatttaacct
    65641 cggtgaagag ctgttcagtg cggtagatga aacctggcag agcctgaaag acaccttcag
    65701 ccttagcgta ctgatgaacc ccattgaagc cagcaaaggc gacggcgaaa tgggtaccgg
    65761 ggcgatgggc gtgatggatc agaaattcgg tagcgcagct gccgcataca gctacctgat
    65821 tttcgtcctg ctgtacgtac cgtgcatctc ggtgatgggg gctatcgcgc gtgaatcaag
    65881 ccgtggctgg atgggcttct ccatcctgtg ggggctgaat attgcttact cactggcaac
    65941 attgttctat caggtcgcca gctacagcca gcatccaact tacagcctgg tgtgcattct
    66001 ggcagttatc ctgtttaaca tcgtggttat cggtctgctg cgccgcgcgc gtagccgggt
    66061 agatgtcgaa ctgctggcaa cgcgcaaatc ggtaagcagt tgctgtgcgg ccagtaccac
    66121 tggcgattgc cactaaggac acgacaatgg cttcgctcat tcaagtgcgt gatttactgg
    66181 cgttacgggg ccgtatggaa gcggcccaga ttagccagac actgaacact ccgcagccaa
    66241 tgattaacgc catgctgaaa cagctagaaa gtatgggaaa agcagtgcgg attcaggaag
    66301 aacctgacgg ctgtctttcc ggcagttgta aaagctgccc ggaaggaaaa gcctgtctgc
    66361 acgaatggtg ggcgttgcgt taaccttgtt tcactgcctg ttttgaaagc ccggtatgcg
    66421 tttgcatccg ggcttttttg cgtgcggctt tccataaaaa tgcaaccctt gcagcgcgac
    66481 gtaagttcct ctgaaagcgt ctcgcaggga tgaaaactcg ctaatgcaca ggtgtggagt
    66541 ggcgcgtaga gtcgcggcat tcaaataaca ggtgaaggaa cgccatgagc aaaaagcaga
    66601 gttccacccc acacgatgcg ttacgccatt gccgcaatgc aaaatcacct tgatgccgga
    66661 tacaaaacat tgccgatggt ggtgccattg ttgttttatc acggtattga aagcccctat
    66721 ccctattcgc tgtgttggct ggattgtttc gccgatccca acctggcaag gcagctttat
    66781 gcctctgcat ttccgctgat tgatgtcacc ctcatgcctg atgatgaaat catgctgcac
    66841 cgacgtatgg cgctactgga gttaattcaa aaacatattc gtcaacgcga cctgatgggg
    66901 ctggtagagc aaatggcctg cttattaagt agtggatacg ctaatggcag acaaatcaaa
    66961 gggctgttta attacatact gcaaaccggc gacgcggtac gttttaacga ttttatcgat
    67021 ggtgtggcca aacgttcacc aaaacacaag gtgagtttaa tgactattgc ggaaagattg
    67081 cggcaggaag gggaacaatc caaagccttg catatagcaa aaataatgct tgaatccgga
    67141 gtccctcttg cagacatcat gcgctttacc ggggtgtcag aagaagagtt ggctgcagcg
    67201 agccagtaaa gttctgtccc gccatttcga aagccaccta caccctctgc ttcaacgcca
    67261 ccagcagatg acaaaactcg tccggatgtg aaataaacgg cgcatgggcc gctttggcga
    67321 agatatatga ttcgctgtga ggccaaagtt tatccagcat cggcaccact ttgcgcggca
    67381 ccagaccgtc gagatagcca tacaatcgca aaaacggcat gggcacgttt tgcagcggct
    67441 gacggagatc gaccgttttc aggatttcca gcccgccatt aagcacgtca acctccggca
    67501 tcggtaacgc cagaacggtt ttcttcaacg cccgcgcatc ctggcgcgcc gtttcagtac
    67561 ccatggtttg taacgccagg aaccgctcca ctgtacgctg aaaatcatca ctgagttgct
    67621 gctgaaatcc cgccagcacg tccggtttta tccccggcca ctcgtcgcga gcactaaaac
    67681 aaggtgacga cgccacggtg accagcgcca gaacccgctc gggatgggtt aacgcaatct
    67741 ggcttgccac cagcccgccc agactccagc ctaaccaaat ggctttatct ggtgcctgtc
    67801 gcagcacgac ttcggccata tcagcaagtg atagcgcacc aaatccccgg ctacgcccga
    67861 agccgggcag gtcaacaagg tgcagcgtaa aatgcgagct aagttcctcg tcaatgcaac
    67921 gccacacttc ggcattcagt ccccatccgt gcagcagcac aagatgaaca ttcccctgac
    67981 ctttggtctg ccaccagatg ttattcatcc gctattgttc tctttttact tacaaggatg
    68041 aacatatgct aacagtaccg ggattatgct ggctatgccg aatgccactg gcgttaggtc
    68101 attgggggat ttgttcagtc tgctctcgcg ccgcccgtac agataaaacg ttatgcccac
    68161 aatgtggatt acccgccaca cactctcatc ttccctgcgg tcgctgcctg caaaaaccgc
    68221 tgccctggca aagactggtc acggtcgccg actatgcgcc gccattaagt ccgctcattc
    68281 accagcttaa gttttcccgg cgcagtgaaa tcgccagcgc cctgtcacgt ctgttactat
    68341 tggaagtctt acacgctcgt cgcaccaccg gcttgcaact gcctgatcgc atcatcagcg
    68401 ttccgttatg gcagcgccgt cactggcgtc ggggatttaa tcagagcgat ttgctgtgtc
    68461 agccgttatc acgctggcta cactgccaat gggatagcga agccgtcaca cgaacacggg
    68521 ccaccgcgac ccagcatttt cttaacgccc ggctacgcaa acacaacctg aaaaatgcct
    68581 ttcgccttga attgcctgtg caaggtcgcc atatggtgat tgtggatgat gtcgttacca
    68641 ccggaagtac cgtcgcagag attgcgcagt tgcttttacg caatggtgcg gcgactgtcc
    68701 aggtctggtg cctttgtcga accttgtaga gcctcgatga tgggcgtatt ataaccaact
    68761 aaaatagtca actattaggc cattactatg atccgtattt ccgatgctgc acaagcgcac
    68821 tttgccaaac tgctggcaaa tcaggaagaa gggacacaaa tccgcgtatt tgtgattaac
    68881 cctggcacgc ctaacgctga atgtggtgtt tcttattgcc cgccggacgc tgtggaagcc
    68941 accgacaccg ccctgaaatt tgacctgctg accgcgtatg ttgatgagtt aagcgctcct
    69001 tacctggaag atgcagagat cgattttgtt accgaccagt tgggttcgca gctgacgctg
    69061 aaagcgccta acgccaaaat gcgtaaagtg gctgacgatg cgccgctgat ggagcgcgtg
    69121 gagtatatgc tgcaatcaca gatcaaccca cagcttgctg gtcacggtgg tcgcgtttcg
    69181 ctgatggaaa tcaccgaaga cggttacgcc attttgcaat ttggcggtgg ctgtaacggt
    69241 tgttccatgg tcgacgtcac cctgaaagaa gggatcgaaa agcagttgct gaacgaattc
    69301 ccggagctga aaggtgtacg cgatctcacc gaacaccaac gcggcgagca ctcttactac
    69361 taagttctct ccccttttgt cgattgcccg atgtgacgct aaagcgtctt atcgggcctg
    69421 tacgttgcac gagagtctga cgttagcgca gaattacctc agcaaatcca atctcttcgt
    69481 ttccccacga taatatgacc aacctctcat aatttaaatt taccccgctc tggtgattct
    69541 caagcacgta atgttacccg tatcattctc atgggcacca aacacactca taacatctga
    69601 ctacactaac tcgttttagg ggtcatcagc cgtggtgccg taacatctct gttcccagtt
    69661 gggacaacca ggattttccg tcagaaacct gacgttaccc ataacaaatt aaaggccagg
    69721 taaatcatgc cattagtcat tgttgctatc ggtgtaatct tgttattact cctgatgatc
    69781 cgcttcaaaa tgaacggctt catcgctctc gtcctcgtgg cgcttgctgt tggattaatg
    69841 caaggaatgc cgctggataa agttattggc tccatcaaag ctggtgtcgg cggaacgctc
    69901 ggtagccttg ccctgatcat gggttttggt gcgatgctgg gcaaaatgct ggcagactgc
    69961 ggtggtgcac aacgtatcgc taccacactg attgccaaat ttggtaaaaa acacatccag
    70021 tgggcggtgg tactgaccgg ttttaccgtt ggttttgccc tgttctatga agtgggtttc
    70081 gtgctgatgc tgccgctggt gtttaccatc gcggcttccg cgaatattcc actgctgtat
    70141 gttggtgtac caatggcggc tgcactgtct gtgacccacg gcttcctgcc accgcatccg
    70201 ggtccgactg caattgccac cattttcaat gccgatatgg gtaaaactct gctgtacggt
    70261 actattctgg caatcccgac cgtgattctc gccggtccgg tttacgctcg cgtgctgaaa
    70321 ggtatcgata agccgatccc ggaaggtctg tacagcgcga aaaccttcag cgaagaagag
    70381 atgccgagct ttggcgtcag cgtctggacc tctctggtgc cggtggttct gatggcgatg
    70441 cgtgcgattg ccgaaatgat cctgccgaaa ggtcacgctt tcctgccggt ggcggagttc
    70501 ctcggtgacc cggtaatggc cacgctgatt gccgtgctga ttgcgatgtt cacctttggt
    70561 ctgaaccgtg gtcgttcaat ggatcagatt aacgacacgc tggtttcttc catcaaaatc
    70621 atagcgatga tgctgttgat tatcggtggt ggcggtgcgt tcaagcaggt gctggtagac
    70681 agcggcgtgg acaaatacat tgcttccatg atgcacgaaa ccaacatttc tccgctgctg
    70741 atggcctggt cgattgctgc cgtactgcgt atcgcgctgg gttccgcaac cgtcgcggca
    70801 atcactgcgg gtggtatcgc ggcaccgctg attgcaacga cgggtgttag cccggagctg
    70861 atggttattg cggttggttc cggtagtgtg attttctctc acgtgaacga tccgggcttc
    70921 tggctgttca aagagtactt taacctgact atcggcgaga ccatcaaatc ctggtcgatg
    70981 ctggaaacca ttatctcggt gtgcggtctg gtaggctgtc tgctgctgaa tatggtgatt
    71041 tgataatact gatggccgga tgcggcgtga acgccttatc cggcctacga ttcggcatta
    71101 attagtaggc cggataagac gcgtcaagcg tcgcatccgg catcaaccgc actctacttc
    71161 ttcttcgctg ccgctctgcg ccgtctgtcc aaatccttca gcaacttgtt cacaccatca
    71221 tcggcaaaca tcgactcaag cgttgcggaa agtttacgtc gccagttttt atactggtaa
    71281 ctggtgccgg gaatatttac cggatctgcc atatccagcc agtcttccgg ctgtagccct
    71341 aacagggcgc tgttgctatc ggcgatgtag cgctgtaagc cacgattcag cgtcggcgtc
    71401 atcgacatca acgatgcctt atgcccggca cgttttggca gacaaccata tttatgcaga
    71461 gcatccagta gtccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact
    71521 tcatccggat acaaccccag cgttttgccc agcgttagat ccccgctctc ccagtagccg
    71581 cgtagcgtag gcaggtcgtg tgtcgccgca accgccatcg actgctctgg gtacgctttc
    71641 ggcgcgcgga atgtcttctc gtggtcgttt tcgaaataga gcactttgta ggagtacaca
    71701 ccgctgctgc gcagcttacc gacaatctct accggtacgg taccgagatc ttcaccaatc
    71761 accatacagc gatgacgctt actttcgagt gccagaatcg agagcagatc atccaccgga
    71821 tagtgaacat atgccccctg atctgccgtc tcgccatacg gtatccacca caaacgcagc
    71881 atcgacatca catggtcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc
    71941 tcgataaacg gttcataggc acgcgcggtg ataatatgcg ggtccattgg cggcaatccc
    72001 cagttctgcc ccaacgggcc gaggatatcc ggcggtgctc caaccgacgc tttcaggcaa
    72061 tacaactccc ggtcgcacca ggtttctgct ccaccctctg ctacaccaac ggccaggtca
    72121 cgatacaagc caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg
    72181 ctgtaagcca gccactgcaa ccagagataa aaatcaacgt catcgcgatg ttcttcgcag
    72241 aactggcgca cttctggtga atccacgttc tgatacatct ctggccatgc aggccagccc
    72301 cagcgcattt cgtcctcttt cacttgctgg gcatgtagtg cgtcaaacgc cgcctgccag
    72361 aacaggctgt cgccttgctc tgcaacaaac tggcgaaacg cggccatctg ttcatcatca
    72421 cgttgcgcga aacctttcca cgccattcgt aatgctgtca tttttagggc ggtaaccgtg
    72481 gagtaatcga cccagtcggc atcccgcgcc tgttgcagcg tctgttgcgt ggtcggcaac
    72541 ttccaccaag cctgagcctc ttcgctaaga tggaaatctt caacggcgtt aacgtcgata
    72601 taaatcacat tcagccaacg gcgagaagac gggctatacg ggctggaact ttccggattt
    72661 gccggataga gcgcatgaat cgggttcaga ccgatgaacg acccgccacg ttttgccaca
    72721 tccaccagca tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc
    72781 agtgtataaa gctgaacgca ggcaccccac agcttttgtt tgttcagcaa cgcctgcggt
    72841 tcgtaacagc gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggta
    72901 agcgtcagcg tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc
    72961 atcacatggc ctttgtactg ctttccttct tcggtggtca gcagccagct atattcgccg
    73021 ctgccctcca ccaccatcgg catttttttg ccgctggtat aaaccatgac attcgggact
    73081 ggcgttaccg ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg
    73141 gtttcggcgc taatcgactg cggtttaccg tgggcattga tgtaattggg gctgatcccc
    73201 gccgccagcg cggcattatc cagacgtttg ctttccatcg agcttcctta gcgttttgcc
    73261 tgccagatac gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg
    73321 gcggtattga ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc
    73381 tgcttttgtg cctctacata agcggcgaaa tctgccatca ccagatacgg atcgccgccc
    73441 tgtttgccga tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtattta
    73501 ccgctttcca gctctttcaa tactgcgtcc agcaccttat ctttcttccg ccatttcacc
    73561 gggtcgtagc ctttggccag aattgccttc acttgttcca cggtatgacc aaaaataaag
    73621 atattttctt caccgacttt ctcagcgatt tcaacgttcg caccatccag cgtaccgaca
    73681 gtaagcgcac cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca
    73741 gtcgaaattt gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacacaa
    73801 taatccggca agaaaaccac cttcaacttg tcgccaacca gcggatcgtt gttgatcacg
    73861 tcagccactt tgttgatcgc aaagataata tttttcgcca ggtagtagcc cggtgctgct
    73921 ttcgcgccga agaggaagac gcgcggtacg cggtcagact gcgggttttc acgaatttct
    73981 ttgtacaacg ccagaatatg cagcagattc agatgctggc gtttgtactc atgcagacgt
    74041 ttgatctgaa tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca
    74101 aactctgcca gacggacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc
    74161 gcatcatcag cgaatttttc cagattgatc agttgatcga gatcgttagc ccactctttt
    74221 ttcagtgatt tatccaacag agccgccagt gccgggttgc actgtttgat ccagcgacgt
    74281 ggggtaatac cgttggtgac attatggaat ttgttcggcc atagctggtg atattccggg
    74341 aacagatctt tcaccaccag atctgagtgc agcgccgcaa caccgttcac cgcgaaaccg
    74401 ccaaccacac acaggttcgc catatgcact tgtttgtcgt gtaccaccgc cagtttggcc
    74461 cacacttttt catcgcccgg ccaggttttc tccaccagcg ttttaaagcg agtattaatt
    74521 tcgttaataa tctgcatgtg gcgcggcagt aagcctttca ccagtttcac atcccagcgt
    74581 tccagcgctt ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtgatggcc
    74641 caggcgtcat cccagctcat ctggtgctca tcgatcagca cgcgcagcag ttctggaatc
    74701 gcgatagttg ggtgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcagt
    74761 ttacgccccg ccagatgatg gcgacgcaaa atatccgcta ccgagcaggc acactggaag
    74821 tattgctgca tcaggcgcaa ctttttaccg gcagtatggt tgtcgtttgg atagagaact
    74881 ttggtcagtt tttccgcgtt gatgccctgc tgttcggcac gcaggaaatc accgtcgtta
    74941 aatttagtca gatcaaacgg atgcgcatgc gtcgcctgcc acagacgcag tggctgcgcc
    75001 acgccattac gatagccgac aacggggaga tcccacgctt gaccggtaat ggtaaactcc
    75061 ggctcccagc gtccatcttt cgtcacttta ccgccaatcc ctacctgcac atccagtgct
    75121 tcgttgtggc ggaaccacgg gtagttaccg cgatgccagt catccggcgc ttcaacctgt
    75181 ttgccatcga caaatgactg gcggaacaag ccatattgat aattaaggcc gtagccagta
    75241 gctgactgcc cgacagttgc cattgagtcg aggaagcacg ccgccagacg tcccagacca
    75301 ccgttcccca gcgccgggtc gatctcttct tccaacaggt cagtcaggtt gatgtcataa
    75361 gccttcaacg aatcctgtac atcctgatac cagccgagat tcaacaggtt gttgcccgtc
    75421 aggcgaccaa tcaaaaactc cattgagatg tagttaacat gtcgctgatt cgccactggc
    75481 ttggcgaatg gctgagcacg cagcatttcg gccagtgctt cgctcactgc cagccaccac
    75541 tggcgaggag tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc
    75601 gcttcctgaa attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta
    75661 gattttcaac acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg
    75721 cgggggatta ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcgaccactt
    75781 aaagctagtg caatccacag gattagcagc aaatcaatgc cataccgcgc agaaaatctg
    75841 tatctaagtg caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa
    75901 ttaagtaaag gagtgaaaca gtttcacaag taaaatatca agtgtgctcc atctcattct
    75961 taatagattt attaagatca tctttttaga tggcactttc atcagaaatg aagaagaaac
    76021 ccttgcttaa atgaaactga tgaacataag gaaaataccg tacaacccag tattcacgct
    76081 ggatcagcgt cgttttaggt gagttgttaa taaacatttg gaattgtgac acagtgcaaa
    76141 ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc gaccttataa
    76201 ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt gaagtgatta
    76261 actatgctga ttccgtcaaa attaagtcgt ccggttcgac tcgaccatac cgtggttcgt
    76321 gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct gatcacaagt
    76381 cctgcgggct acggaaagac cacgctcatt tcccagtggg cggcaggcaa aaacgatatc
    76441 ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag ctatctcatt
    76501 gccgccgtgc aacaggcaac caacggtcac tgcgcgatat gtgacacgat ggcgcaaaaa
    76561 cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct ggcggaatgg
    76621 catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc agtgatccac
    76681 gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcacccttgt ggtgttgtca
    76741 cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccagct gctggaaatt
    76801 ggcagtcagc aactggcatt tacccatcag gaagcgaaac agttttttga ttgccgtctg
    76861 tcatcgccga ttgaagctgc agaaagcagt cggatttgtg atgatgtttc cggttgggcg
    76921 acggcactgc agctaatcgc cctctccgcc cggcagaata ctcactcagc ccataagtcg
    76981 gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt cgatgaggtt
    77041 ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat tttgcgctca
    77101 atgaacgatg cactcatcac ccgtgtgacc ggcgaagaaa acgggcaaat gcgcctcgaa
    77161 gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga gtggttttgc
    77221 tatcacccac tgtttggtaa cttcctgcgc cagcgctgcc agtgggaact ggcggcggag
    77281 ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt tcccagcgaa
    77341 gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat tctgcttaat
    77401 cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc gcttaaggcc
    77461 ctgccgtggg acagtttgct ggaaaatccg cagttggtgt tattgcaggc gtggctgatg
    77521 caaagccagc atcgctacgg cgaagttaac accctgctgg cccgcgctga acatgaaatc
    77581 aaggacatca gagaaggcac catgcacgca gaatttaacg ctctgcgcgc ccaggtggcg
    77641 attaacgatg gtagtccgga tgaagcggaa cggctggcaa aactggcact ggaagagctg
    77701 ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga agtgttgcac
    77761 tgcaaaggcg aattgacccg ctcactggcg ttaatgcagc aaaccgaaca gatggcacgc
    77821 cagcacgatg tctggcacta cgctttgtgg agtttaatcc agcaaagtga aattctgttt
    77881 gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca gctgatcaac
    77941 gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcattcg tgcgcagctg
    78001 ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgcagcgg gattgaagtc
    78061 ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat tcaatgctcg
    78121 ctggcccgtg gtgatttaga taacgctcgt agccagctta accgtctgga aaacctgctg
    78181 gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg ggtgatttac
    78241 tggcaaatga ccagcgataa agccgccgct gccaactggt tgcgtcatac ggctaaaccg
    78301 gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg tgcacaaatc
    78361 ttgctgggcg agtttgaatc tgcggaaatt gtcctcgaag aactcaatga aaatgcccgc
    78421 agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca actgtactgg
    78481 caggccggac gtaaaagtga cgcccaacgt gtgttgctgg acgcattaaa actggcgaat
    78541 cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca acaactgcgc
    78601 cagcttattc aactcaatac gctgccggag ctggaacagc atcgcgcaca gcgtattctg
    78661 cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa tttcgttgaa
    78721 cgtctgctaa atcatccgga agtgccagag ctgatccgca ccagcccgtt gacccaacgt
    78781 gaatggcagg tcctggggct gatttactcc ggttacagca atgagcaaat tgcaggcgag
    78841 ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca aaaactcggt
    78901 gtcgcccacc gtcaggccgc ggtacaacac gcgcagaaac tgctgaagat gatgggatat
    78961 ggggtatgag tttagccgga taatgcgcca gatccggctt acatctttac atcattcaat
    79021 gctcacccgc gttacgccat ctgtttctat cagactaaac cgcactggca agaaacgctc
    79081 caccaccgcg atattggtct gcagatggca tgagggatgg gcgaccgtaa attctcccgc
    79141 ccccgccagc gccatcggta gcaccagttg gtcggcgaga tattccccca ccgccgccgg
    79201 gcttgccagg tagcgtttca cctctttcac caactgtgcc gcgaccacct cggcactgac
    79261 gcgcttttca ccgacgacaa aaaagcgttc ggtgatattt tcactttcga cttcaagcga
    79321 gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt catgcaagga
    79381 aaaactaccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca caccagccag
    79441 tagcacctct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca agctattaaa
    79501 cgatgccacc ggtgagactt ccgttgccac cacgccgcct ccggcaggat aaaaaccgtg
    79561 acgtaacagc gtggtttgct gatgaactcc tattttcgcc agcagcggct ccagcacccg
    79621 gcggataaaa tccgcaggcg gggccgacgg gttatcggtg ccgccgctca cttcaacacg
    79681 cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca gcgtacaact
    79741 tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag gtcggaagac
    79801 cagacgctgc gaccccagct cagcgccttc caccgttgcc ctacaaattt ccgcagccgc
    79861 ttttaccgcg gtcagatgtt ggcgcaacag ccccggtttc gcccgcccgg cacgaatgcc
    79921 ggtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc gcaggatctg
    79981 cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca tttatccttt
    80041 tacgcacacc acctgacgca gggtatagat aacttccacc agatcgcttt gtgccgccat
    80101 caccgcatca atatctttat acgccatcgg gatttcgtcg atcacctcgg catctttacg
    80161 gcactccaca tgcgcggtgg cacggatttg atcttccaca ctgaacagtt ttttcgcttt
    80221 agtacggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact cttcatttcc
    80281 cagcccacgg acgataaagc ttttcgcccc catcgaaccg ggaataattc catattgacc
    80341 agcacgcgca gacaccgcgc ctttacgcgt cacatagatc tcttcgccaa agtgctgttc
    80401 tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt gtggctgttt
    80461 taccgttttc tgcgtaacgc tctgcaacgc cgttaccacg ttttccatca tcgcatcgcg
    80521 gttaaggctg gcaaaaagct gcgcccaggc tacggctttc aggtaatcat caaagtattc
    80581 cgtaccttcc ataaagtacg ccaggtcacg cgatggcaac gtttcaagcg tttcctgcat
    80641 ctctttttgc gccagatcga taaaataagt tccgatggcg ttaccaattc cgcgtgaacc
    80701 ggagtgcagc ataatccaca cctgatccga ctcatcaagg caaatttcaa taaagtggtt
    80761 acccgtaccc agcgttccca ggtgtttata gttattagtg ttaaggaaac gcggatattt
    80821 ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga cgttaacagg
    80881 cggattttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgtc cgtgcggtac
    80941 ggccgtttca atcgcctgac gcagctctgc caggttttct ggcaggtccg ccgccgttaa
    81001 cgcggtacgc agtgcgttca ttccacagcc aatatccacg cccaccgccg ccggaataat
    81061 cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt gtacatcagg
    81121 cattaccgca atatgtttga aaataaacgg catcttcgcg gtattaataa gttgctgacg
    81181 cgcatcggct tctaccggca cgcctttggt ccacattttt accggagcat tttcagtggt
    81241 cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgctgtcct gataattgca
    81301 accgtcgtgc cagaaaatca aataagcatc ttaatttttt aattcattgt ttttaaaaat
    81361 attatatttt tacgtccgta aacggagatt tcccgcaaag ccgatttacc gataatgaaa
    81421 tctcgtcttt tataaggata tctaagatgc gtaaaacagt ggcttttggc tttgtcggaa
    81481 cggtgctgga ttatgccggg cgcggcagtc agcgctggtc aaaatggcgt ccgacactct
    81541 gtctatgcca gcaagaatcg ttagttatcg atcggctgga attattgcac gacgcccgct
    81601 cgcgctcgct attcgaaacg cttaaacgcg atatcgccag cgtttcgcca gaaacagaag
    81661 tggtgggcgt tgagatagaa ctgcataacc cttgggattt cgaagaggtt tacgcctgcc
    81721 tgcatgattt cgcccgtggt tacgcgttcc aacctgataa agaagagtat ttaattcaca
    81781 tcaccaccgg cacccacgtc gcgcagattt gctggtttct gctggcagaa gcgcgttacc
    81841 tgcccacccg gttgatacaa tcttcaccac cgcgcaaaaa agagcggccc gatagccctg
    81901 gcgcagtgac gattatcgat ctcgatttaa gccgttataa cgccatcgcc agccgctttg
    81961 ccgaggaacg ccagcaaacg cttgattttc ttaagtccgg tattgccacg cgtaactccc
    82021 acttcaaccg catgattgag cagattgaaa aagtggcgat aaaatcccgc gcaccgatcc
    82081 tgcttaacgg cccgaccggt gcaggtaagt catttctggc acgacgtatc ttcgagctaa
    82141 aacaggcgcg gcatcagttt agcggcgcgt ttgtggaagt gaactgcgcc accctgcgcg
    82201 gcgataccgc catgtcgacg ctgtttggtc atgtgaaagg cgcgtttacc ggggcgcggg
    82261 aatcgcgtga aggattatta cgcagcgcca acggcggaat gttgtttctt gatgagattg
    82321 gcgaactggg cgcagacgaa caggcaatgc tgctgaaagc cattgaagag aaaacctttt
    82381 acccgtttgg cagcgatcgc caggtgagca gtgattttca gcttatcgcc ggaacggtgc
    82441 gcgatttgcg ccagttggtt gccgaaggca aatttcgtga agacctgtac gcgcgaataa
    82501 atctctggac cttcaccttg ccaggattac gccaacgcca ggaagatatt gaaccgaacc
    82561 tggattatga agtggagcgc cacgcctcac tcactggcga cagcgtgagt tttaacaccg
    82621 aagcgcggcg tgcctggctg gcctttgcga cctctcccca ggcaacatgg cgcggtaact
    82681 ttcgcgaact ttctgccagc gtcacgcgga tggccacctt tgccactagc ggacgcatca
    82741 cactggatac ggtggaagat gagatcaacc gtctgcgcta taactggcag gagagtcgcc
    82801 cctccacgct tacggcgttg cgggcgcaga ggcagaaaac atcgatctct tcgaccgtat
    82861 gcaactggaa cacgttatcg ctatctgccg ccaggcaaag tcgctttctg ctgccggacg
    82921 tgaactgttt gacatttcgc gccagggtaa agccagcgtc aatgacgcgg atcggctacg
    82981 caaatacctg gcgcgttttg gtctgacatg ggaagcaatg caggatcagc acagctccag
    83041 ttgaatatga tggtccttca gcacctgcat cacgctcgca ggcggcatcg tgtcggtgta
    83101 gacagcatcg accatgctga tgctacccat attgaccatt gcgttacggc caaattttga
    83161 gtggtcgaca accagcataa cgtggcgcga gttctcaatg atggcgcgtt tggtgcgaac
    83221 ttcgtggtaa tcgaactcca gtagcgagcc gtcgctatcg atgccgctta tccccagaat
    83281 gccgaaatcg aggcggaact gggagataaa atcgagcgtt gcttcgccaa tgatcccgcc
    83341 atcacggcta cgcaattcac caccggcaag aatgatacgg aaatcctctt ttaccatcag
    83401 cgtgttagcg acgttgagat tgttggtcac aatgcgcaaa ttgctgtgat taagcagcgc
    83461 gtgcgccacc gcttccggcg tggtgccgat atcgataaac agcgtcgagc cattgggaat
    83521 ttgctccgcc actttgcggg cgatgcgctc tttttcttcg gtctgggtgg ccttgcgatc
    83581 gtgccacggc gtgttaaccg aactggaagg cagcgccgca ccgccatgat ggcgcaggat
    83641 caggttttgc tctgccagct cgttgagatc gcggcgaata gtctgcgggc taacggagaa
    83701 atgctctacc agctcctcgg tgctgacata accctgctgt ttaaccagtt cgataatacc
    83761 gttgtgacgt tgtgtttgtt tcattgataa atccctggaa ttattttcgt tttcgcgcat
    83821 tgagcgaatc aacaaaagcc atcgctaaac ccacggctaa cccggcgatg tgtgctccgt
    83881 tcgccatcga catcccaaac aaatcaaacc atccggcgac aatccagatc aacgcaaaga
    83941 taattaaccc acgttgcagg taaatgccac tttgcggatc gcgttcgcca cgtagccaga
    84001 cgtagcccat cagcgcatac accacgccag aaagcccgcc aaaccacggc ccgctgaatt
    84061 tttgctgcac atagccgctt aacagggcgc taatgagagt aatgacaatt agcttaccgc
    84121 taccgaggcg tttttccacc gcaccgccga gataccacca ccagagcagg ttaaagagga
    84181 tatgcatcag cgagaagtgc attaacgcgt gggtgaagta acgccagaac tcaaatttca
    84241 gcgtcggatc gaatggccag gccagccata acatcacttc ttgatcgccg agaatttgca
    84301 tggcgataaa caccaccacg caggcgatca tcatcaccca ggttaccgga cctgcgcgtt
    84361 cacgcaaggc agcaaagaaa ggataacggc gataatgcag gccactgtcg gtatgaccgg
    84421 actgccaact ggccgccaga taacgcggat ctgccgggtt tccgagaaaa cgcgccagct
    84481 ccgcccgcac gcgttcggcc tgggactcat ccgccagcca gacatcgctt tggttatgtt
    84541 gttgaatcgt gaggataaca ccctgcgtcg ccatgtaatc aacaaacgcc tgcgccacgc
    84601 gggggttagc aaaagaggta atcatcaaca tcgttgctgt cgcttattcc acacaaaagg
    84661 ggacagtata aagcgttacg cgccgtacgc cacctctgcg ggaaactggc gctgccaggc
    84721 ttcaaagccg ccatcaatgc tgtagaccac atcgtagccc tgttgcagca gatactgcgc
    84781 cgcgcctttg ctgctattac cgtggtaaca catcaccatg actggcgtat cgaagtcgtt
    84841 atcacgcata aacgcgccca gtgtgtcgtt agttaaatgg aaagcctgcg ccgcatgccc
    84901 cattgcgaaa ctctgtggat cgcgaatatc gaccagcacc gcctcttttt cctgcaactt
    84961 ctggtgcgcg tcggcaacgt taatacattc gaactgatcc atgcgtctct ctttctttac
    85021 aaacaagtgg gcaaatttac cgcacagttt acgtcgaagc ggcagataaa cgccataatg
    85081 ttatacatat cactctaaaa tgttttttca atgttaccca aagcgcgatt ctttgctaat
    85141 atgttcgata acgaacattt atgaacttta acgaaagtga atgagggcag catggaaacc
    85201 aaagatctga ttgtgatagg tggcggcatc aatggtgctg gtatcgcggc agacgccgct
    85261 ggacgcggtt tatccgtgct gatgctggag gcgcaggatc tcgcttgcgc gacctcttcc
    85321 gccagttcaa aactcattca cggtggcctg cgctaccttg agcactatga attccgcctg
    85381 gtcagcgagg cgctggctga acgtgaagtg ctactgaaaa tggccccgca tatcgccttc
    85441 ccgatgcgtt ttcgcctgcc gcatcgtccg catctacgcc cggcgtggat gattcgtatt
    85501 ggtctgttta tgtacgatca tctgggtaaa cgcaccagcc tgccgggatc aaccggtttg
    85561 cgttttggcg caaactcagt gttaaaaccg gaaattatgc gcggattcga atattctgac
    85621 tgttgggtag acgacgcccg tctggtactc gccaacgccc agatggtggt gcgtaaaggc
    85681 ggtgaagtgc ttactcgtac tcgtgccacg tctgctcgcc gcgaaaaagg cctgtggatt
    85741 gtggaagcgg aagatatcga taccggcaaa aaatatacat ggcaggcgcg cggcctggtt
    85801 aatgctaccg gcccgtgggt gaaacaattt ttcgacgacg ggatgcatct gccttcacct
    85861 tatggcattc gcctgatcaa aggcagccat attgtggtgc cgcgtgtgca tacccagaag
    85921 caagcctaca ttctgcaaaa cgaagataaa cgtattgtct tcgtgatccc gtggatggac
    85981 gagttttcca tcatcggcac caccgatgtg gaatacaaag gcgatccaaa agcagtgaag
    86041 attgaagaga gtgaaatcaa ttacctgttg aaagtgtata acacgcactt taaaaagcag
    86101 ttaaaccggg acgatatcgt ctggacctac tccggcgtgc gtccgctatg tgatgatgag
    86161 tccgactcgc cacaggccat tacccgtgat tacacccttg atatccatga tgaagacggc
    86221 aaagccccgc tgctgtcggt attcggcggt aagctgacca cctaccgcaa actggcggag
    86281 cacgcgctgg aaaaactgac gccatattat cagggcattg gtccggcgtg gacgaaagag
    86341 agcgtgctgc cgggtggtgc cattgaaggc gaccgtgacg attacgccgc ccgcctgcgc
    86401 cgccgttacc cgttcctgac cgaatcgctg gcacgtcact acgcgcgcac ttacggcagc
    86461 aacagcgaac tgttgctcgg caatgcaggg gcgataagcg acctcggtga aaatttcggt
    86521 catgagttct acgaagcgga gctgaaatat ctggtcgacc acgaatgggt ccgccgcgcc
    86581 gacgatgccc tgtggcgtcg cacgaaacaa ggcatgtggc taaatgcgga tcaacaatct
    86641 cgtgtgagtc agtggctgat ggaatatacg cagcagaggt tgtcgctggc gtcgtaagga
    86701 tgatgcccgg ttgcgaagtg caaccgggct atttgaattc cttagactat tcattgtaaa
    86761 taattgaata acggccccag agctaaaatc gccaaactta agcacgttac ccaatcgccc
    86821 cacttcttcc tatggcttct atttttgcta cgccactggc aataatatcc caacgcaaaa
    86881 ccaagctggg cactaatcgg gccgacaaaa tcagtccacg gctgcggcca aaaaggggaa
    86941 aaattcaggg gaagccagac aaatgtcaga aagaaatttc ggatattttc cagcaacgct
    87001 gatttctctg gagaaatata aagaatgaaa ttcgagagaa ttaaccagaa atagaccgga
    87061 gaaaagacca gccagcaatg tagcctgttt tttaattcag gaggcgttct gcgggagaat
    87121 tgccaaccga tgccacccat tacactgaaa aaaagtgcat attcaaccaa taactcaagt
    87181 tgtaattcct gaagagaaat acgcacaacc aggagcggaa aggagaatat tatttccgcc
    87241 agaaaagtta acgtgaatat aagtacggct aaggcaataa ccgtcggcca taccaataat
    87301 ggtacgataa caaaaccgag aattacaggg ggaatataaa gccggaattt aatcattttt
    87361 attttcctgg tgattcggat gatgcgtcat acatcgcgca taaacactca tcaatcatcc
    87421 agtcgtcttc ccagatctga tactgtgtgc cctgt
//
LOCUS       DAZGJT010000004        80347 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZGJT010000004 DAZGJT010000000
VERSION     DAZGJT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.004"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..339
                     /locus_tag="ACHKFK_000270"
     CDS             <1..339
                     /locus_tag="ACHKFK_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO9050700.1"
                     /translation="ACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQV
                     QYSMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIEKMDEALAE
                     IGFVFGEQWR"
     gene            336..518
                     /locus_tag="ACHKFK_000271"
     CDS             336..518
                     /locus_tag="ACHKFK_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO9050701.1"
                     /translation="MTHPHDNIRVGAITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLPLS"
     gene            491..682
                     /locus_tag="ACHKFK_000272"
     CDS             491..682
                     /locus_tag="ACHKFK_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9050702.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLTTSL
                     SQKLEMMVAKAEADERDQV"
     gene            759..974
                     /locus_tag="ACHKFK_000273"
     CDS             759..974
                     /locus_tag="ACHKFK_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9050703.1"
                     /translation="MTPQQENALRSIARQANYEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1073..1291
                     /locus_tag="ACHKFK_000274"
     CDS             1073..1291
                     /locus_tag="ACHKFK_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9050704.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            1339..1578
                     /locus_tag="ACHKFK_000275"
     CDS             1339..1578
                     /locus_tag="ACHKFK_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9050705.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRAIRRK"
     gene            1718..1954
                     /locus_tag="ACHKFK_000276"
     CDS             1718..1954
                     /locus_tag="ACHKFK_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9050706.1"
                     /translation="MSRLITLQDWAKEEFGDLAPSERVLKKYAQGKMMAPPAIKVGRY
                     WMIDRNSRFVGTLAEPQLPINANPKLQRIIADGC"
     gene            1944..3086
                     /locus_tag="ACHKFK_000277"
                     /pseudo
     CDS             1944..3086
                     /locus_tag="ACHKFK_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3200..4450)
                     /gene="icd"
                     /locus_tag="ACHKFK_000278"
     CDS             complement(3200..4450)
                     /gene="icd"
                     /locus_tag="ACHKFK_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO9050707.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            4622..5275
                     /gene="rluE"
                     /locus_tag="ACHKFK_000279"
     CDS             4622..5275
                     /gene="rluE"
                     /locus_tag="ACHKFK_000279"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO9050708.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPDNQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVEEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGNYSLDNLANGEWRDATD"
     gene            5285..5746
                     /gene="nudJ"
                     /locus_tag="ACHKFK_000280"
     CDS             5285..5746
                     /gene="nudJ"
                     /locus_tag="ACHKFK_000280"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO9050709.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            5800..6906
                     /gene="mnmA"
                     /locus_tag="ACHKFK_000281"
                     /gene_synonym="trmU"
     CDS             5800..6906
                     /gene="mnmA"
                     /locus_tag="ACHKFK_000281"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO9050710.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKDGTEEPWYVVDKDVENNILIVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDADRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            6942..7583
                     /gene="hflD"
                     /locus_tag="ACHKFK_000282"
     CDS             6942..7583
                     /gene="hflD"
                     /locus_tag="ACHKFK_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO9050711.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDGDALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGTLDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            7587..8957
                     /gene="purB"
                     /locus_tag="ACHKFK_000283"
     CDS             7587..8957
                     /gene="purB"
                     /locus_tag="ACHKFK_000283"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO9050712.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLEELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            9126..9797
                     /gene="phoP"
                     /locus_tag="ACHKFK_000284"
     CDS             9126..9797
                     /gene="phoP"
                     /locus_tag="ACHKFK_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO9050713.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHSEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            9797..11257
                     /gene="phoQ"
                     /locus_tag="ACHKFK_000285"
     CDS             9797..11257
                     /gene="phoQ"
                     /locus_tag="ACHKFK_000285"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO9050714.1"
                     /translation="MKKLLHLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            11333..12454
                     /gene="roxA"
                     /locus_tag="ACHKFK_000286"
                     /gene_synonym="ycfD"
     CDS             11333..12454
                     /gene="roxA"
                     /locus_tag="ACHKFK_000286"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO9050715.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(12503..13729)
                     /gene="pepT"
                     /locus_tag="ACHKFK_000287"
     CDS             complement(12503..13729)
                     /gene="pepT"
                     /locus_tag="ACHKFK_000287"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO9050716.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLANMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            13979..15115
                     /gene="potA"
                     /locus_tag="ACHKFK_000288"
     CDS             13979..15115
                     /gene="potA"
                     /locus_tag="ACHKFK_000288"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO9050717.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            15099..15956
                     /gene="potB"
                     /locus_tag="ACHKFK_000289"
     CDS             15099..15956
                     /gene="potB"
                     /locus_tag="ACHKFK_000289"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO9050718.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            15953..16747
                     /gene="potC"
                     /locus_tag="ACHKFK_000290"
     CDS             15953..16747
                     /gene="potC"
                     /locus_tag="ACHKFK_000290"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO9050719.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFIILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            16744..17790
                     /gene="potD"
                     /locus_tag="ACHKFK_000291"
     CDS             16744..17790
                     /gene="potD"
                     /locus_tag="ACHKFK_000291"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO9050720.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGSAS
                     SIYEEYYQKLKAGR"
     gene            17848..18321
                     /gene="ymfA"
                     /locus_tag="ACHKFK_000292"
     CDS             17848..18321
                     /gene="ymfA"
                     /locus_tag="ACHKFK_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO9050721.1"
                     /translation="MSQDSRVFFRIFFGIGLVLILISVVIFYNQFTYSKDAIHTEGVI
                     VDTVWHSSHSHRTGKNGSWYPVVAFRPTPDYTLIFNSSIGSDFYEDSEGDKVNVYYSP
                     GHPEKAEINNLWVNFFKWGFIGIMGVIFIAVGLLISMPSSKKTRRKRKSRPWKKS"
     gene            18306..19094
                     /gene="ycfZ"
                     /locus_tag="ACHKFK_000293"
     CDS             18306..19094
                     /gene="ycfZ"
                     /locus_tag="ACHKFK_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HFO9050722.1"
                     /translation="MEKIMILLSLLMFLPLTAISKPLIPIMKTLFTDVTGTVPDAEEI
                     AHKAELFRQQTGVAPFIVVLPDINNEASLRQNGKAMLAHAASSMSNVKGSVLLLFTTR
                     EPRLIMITNGQVESSMDDKHLGLLVENHTLAYLHADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENDPPGSTTTLGLFAWAVAFIVFAAFFNYTTRLYYALKFAVAMAV
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIATLLWTSKR"
     gene            complement(19232..20053)
                     /gene="cobB"
                     /locus_tag="ACHKFK_000294"
     CDS             complement(19232..20053)
                     /gene="cobB"
                     /locus_tag="ACHKFK_000294"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO9050723.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQTFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(20069..20980)
                     /gene="nagK"
                     /locus_tag="ACHKFK_000295"
     CDS             complement(20069..20980)
                     /gene="nagK"
                     /locus_tag="ACHKFK_000295"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO9050724.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADRRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLAERLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(21009..22253)
                     /gene="lolE"
                     /locus_tag="ACHKFK_000296"
     CDS             complement(21009..22253)
                     /gene="lolE"
                     /locus_tag="ACHKFK_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO9050725.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTDSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLKLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(22253..22954)
                     /gene="lolD"
                     /locus_tag="ACHKFK_000297"
     CDS             complement(22253..22954)
                     /gene="lolD"
                     /locus_tag="ACHKFK_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO9050726.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFRVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(22947..24146)
                     /gene="lolC"
                     /locus_tag="ACHKFK_000298"
     CDS             complement(22947..24146)
                     /gene="lolC"
                     /locus_tag="ACHKFK_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO9050727.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            24407..25480
                     /gene="ycfT"
                     /locus_tag="ACHKFK_000299"
     CDS             24407..25480
                     /gene="ycfT"
                     /locus_tag="ACHKFK_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9050728.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVICKIF
                     NRLALPLFVLFVLMSVAVNFVPTPWLGMNSVIRNLPYYSLGAWFGTTLMTCVKAVPLR
                     RHLLMASLLTVLAVGAWLFNISLLLSLVSIVVIMKLFYQYEQRFGMRSSSLLNVIGSN
                     TIAIYTTHRILVEIFSLSLIPQMNAVRWSPQVELTLLLIYPFVSLLICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPATVSYSR"
     gene            25608..29054
                     /gene="mfd"
                     /locus_tag="ACHKFK_000300"
     CDS             25608..29054
                     /gene="mfd"
                     /locus_tag="ACHKFK_000300"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO9050729.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLINTGDLENSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAIQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASNRGRYLMIGAAEHGFVDTMRNLALICESDLL
                     GERVARRRQDSRRAINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            29197..30159
                     /gene="ldtC"
                     /locus_tag="ACHKFK_000301"
     CDS             29197..30159
                     /gene="ldtC"
                     /locus_tag="ACHKFK_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO9050730.1"
                     /translation="MMIKTRFSRWLTFFTFAVAVALALPAKANTWPLPSAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(30242..30499)
                     /gene="bhsA"
                     /locus_tag="ACHKFK_000302"
                     /gene_synonym="ycfR"
     CDS             complement(30242..30499)
                     /gene="bhsA"
                     /locus_tag="ACHKFK_000302"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO9050731.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            30739..31371
                     /gene="comR"
                     /locus_tag="ACHKFK_000303"
     CDS             30739..31371
                     /gene="comR"
                     /locus_tag="ACHKFK_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO9050732.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVASALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIARTTLRLWPELLK"
     gene            complement(31433..31972)
                     /gene="ycfJ"
                     /locus_tag="ACHKFK_000304"
     CDS             complement(31433..31972)
                     /gene="ycfJ"
                     /locus_tag="ACHKFK_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9050733.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKA"
     gene            complement(32199..33503)
                     /gene="ndh"
                     /locus_tag="ACHKFK_000305"
     CDS             complement(32199..33503)
                     /gene="ndh"
                     /locus_tag="ACHKFK_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO9050734.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(33902..34444)
                     /gene="ycfP"
                     /locus_tag="ACHKFK_000306"
     CDS             complement(33902..34444)
                     /gene="ycfP"
                     /locus_tag="ACHKFK_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO9050735.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(34467..35492)
                     /gene="nagZ"
                     /locus_tag="ACHKFK_000307"
     CDS             complement(34467..35492)
                     /gene="nagZ"
                     /locus_tag="ACHKFK_000307"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO9050736.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(35503..36327)
                     /gene="thiK"
                     /locus_tag="ACHKFK_000308"
     CDS             complement(35503..36327)
                     /gene="thiK"
                     /locus_tag="ACHKFK_000308"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO9050737.1"
                     /translation="MPFRSNNPLTRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYQPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENIDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(36308..36949)
                     /gene="lpoB"
                     /locus_tag="ACHKFK_000309"
     CDS             complement(36308..36949)
                     /gene="lpoB"
                     /locus_tag="ACHKFK_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO9050738.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(36963..37340)
                     /gene="ycfL"
                     /locus_tag="ACHKFK_000310"
     CDS             complement(36963..37340)
                     /gene="ycfL"
                     /locus_tag="ACHKFK_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO9050739.1"
                     /translation="MRKGCFGLMSLALLVLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(37343..37702)
                     /gene="hinT"
                     /locus_tag="ACHKFK_000311"
     CDS             complement(37343..37702)
                     /gene="hinT"
                     /locus_tag="ACHKFK_000311"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO9050740.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLTHKGL"
     gene            38037..38861
                     /locus_tag="ACHKFK_000312"
     CDS             38037..38861
                     /locus_tag="ACHKFK_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO9050741.1"
                     /translation="MLSTQFNRDNQHQAIIKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYNVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQRLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTMLSAGYEYQRIDVNSPT"
     gene            38952..40225
                     /gene="fhuE"
                     /locus_tag="ACHKFK_000313"
                     /pseudo
     CDS             38952..40225
                     /gene="fhuE"
                     /locus_tag="ACHKFK_000313"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(40285..41718)
                     /gene="ptsG"
                     /locus_tag="ACHKFK_000314"
     CDS             complement(40285..41718)
                     /gene="ptsG"
                     /locus_tag="ACHKFK_000314"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO9050742.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(42013..42810)
                     /gene="ycfH"
                     /locus_tag="ACHKFK_000315"
     CDS             complement(42013..42810)
                     /gene="ycfH"
                     /locus_tag="ACHKFK_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9050743.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(42821..43825)
                     /gene="holB"
                     /locus_tag="ACHKFK_000316"
     CDS             complement(42821..43825)
                     /gene="holB"
                     /locus_tag="ACHKFK_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO9050744.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVQSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(43822..44463)
                     /gene="tmk"
                     /locus_tag="ACHKFK_000317"
     CDS             complement(43822..44463)
                     /gene="tmk"
                     /locus_tag="ACHKFK_000317"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9050745.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEVVMDAIRTTVTNWVKELDA"
     gene            complement(44453..45475)
                     /gene="yceG"
                     /locus_tag="ACHKFK_000318"
     CDS             complement(44453..45475)
                     /gene="yceG"
                     /locus_tag="ACHKFK_000318"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO9050746.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(45478..46287)
                     /gene="pabC"
                     /locus_tag="ACHKFK_000319"
     CDS             complement(45478..46287)
                     /gene="pabC"
                     /locus_tag="ACHKFK_000319"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO9050747.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSITAYPAHYDRLRNEGMMLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDASFSSATLYEYLAPLCERPN"
     gene            complement(46407..47648)
                     /gene="fabF"
                     /locus_tag="ACHKFK_000320"
     CDS             complement(46407..47648)
                     /gene="fabF"
                     /locus_tag="ACHKFK_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO9050748.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(47736..47972)
                     /gene="acpP"
                     /locus_tag="ACHKFK_000321"
     CDS             complement(47736..47972)
                     /gene="acpP"
                     /locus_tag="ACHKFK_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9050749.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(48183..48917)
                     /gene="fabG"
                     /locus_tag="ACHKFK_000322"
     CDS             complement(48183..48917)
                     /gene="fabG"
                     /locus_tag="ACHKFK_000322"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO9050750.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAACGAKVIGTATSENGAQA
                     ITDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(48930..49859)
                     /gene="fabD"
                     /locus_tag="ACHKFK_000323"
     CDS             complement(48930..49859)
                     /gene="fabD"
                     /locus_tag="ACHKFK_000323"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO9050751.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIGKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYIAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(49875..50828)
                     /gene="fabH"
                     /locus_tag="ACHKFK_000324"
     CDS             complement(49875..50828)
                     /gene="fabH"
                     /locus_tag="ACHKFK_000324"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO9050752.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(50896..51966)
                     /gene="plsX"
                     /locus_tag="ACHKFK_000325"
     CDS             complement(50896..51966)
                     /gene="plsX"
                     /locus_tag="ACHKFK_000325"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO9050753.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(52047..52220)
                     /gene="rpmF"
                     /locus_tag="ACHKFK_000326"
     CDS             complement(52047..52220)
                     /gene="rpmF"
                     /locus_tag="ACHKFK_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO9050754.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(52272..52793)
                     /gene="yceD"
                     /locus_tag="ACHKFK_000327"
     CDS             complement(52272..52793)
                     /gene="yceD"
                     /locus_tag="ACHKFK_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO9050755.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            52992..53576
                     /gene="yceF"
                     /locus_tag="ACHKFK_000328"
     CDS             52992..53576
                     /gene="yceF"
                     /locus_tag="ACHKFK_000328"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO9050756.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCVGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(53688..54647)
                     /gene="rluC"
                     /locus_tag="ACHKFK_000329"
     CDS             complement(53688..54647)
                     /gene="rluC"
                     /locus_tag="ACHKFK_000329"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO9050757.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDGEFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            55220..58405
                     /gene="rne"
                     /locus_tag="ACHKFK_000330"
     CDS             55220..58405
                     /gene="rne"
                     /locus_tag="ACHKFK_000330"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO9050758.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAATATPASPA
                     QPGLLSRFFGALKALFSGGEETKPAEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTVETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETVAAEPIVQEAPAPRTELVKVPLPVVAQAAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQHEQEEVQVQPMVTEVPVAAAVKPVVSAPVVEEVAEVVEVPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEDTADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(58600..59553)
                     /gene="flgL"
                     /locus_tag="ACHKFK_000331"
     CDS             complement(58600..59553)
                     /gene="flgL"
                     /locus_tag="ACHKFK_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO9050759.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTESAPFSEADGKYEGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(59565..61208)
                     /gene="flgK"
                     /locus_tag="ACHKFK_000332"
     CDS             complement(59565..61208)
                     /gene="flgK"
                     /locus_tag="ACHKFK_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO9050760.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAGAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNNGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASAEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(61274..62215)
                     /gene="flgJ"
                     /locus_tag="ACHKFK_000333"
     CDS             complement(61274..62215)
                     /gene="flgJ"
                     /locus_tag="ACHKFK_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO9050761.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSERTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTSMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(62215..63312)
                     /gene="flgI"
                     /locus_tag="ACHKFK_000334"
     CDS             complement(62215..63312)
                     /gene="flgI"
                     /locus_tag="ACHKFK_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO9050762.1"
                     /translation="MIKFLSALILLLVITAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(63324..64022)
                     /gene="flgH"
                     /locus_tag="ACHKFK_000335"
     CDS             complement(63324..64022)
                     /gene="flgH"
                     /locus_tag="ACHKFK_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO9050763.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(64075..64857)
                     /gene="flgG"
                     /locus_tag="ACHKFK_000336"
     CDS             complement(64075..64857)
                     /gene="flgG"
                     /locus_tag="ACHKFK_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO9050764.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(65029..65784)
                     /gene="flgF"
                     /locus_tag="ACHKFK_000337"
     CDS             complement(65029..65784)
                     /gene="flgF"
                     /locus_tag="ACHKFK_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO9050765.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTANNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVVGEAGPIAVPEGEEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQARRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(65804..67009)
                     /gene="flgE"
                     /locus_tag="ACHKFK_000338"
     CDS             complement(65804..67009)
                     /gene="flgE"
                     /locus_tag="ACHKFK_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO9050766.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDSLPSVNAFDASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTLDSSDP
                     TGTANPATTLVFNANGVLTSNPTANISTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(67034..67729)
                     /gene="flgD"
                     /locus_tag="ACHKFK_000339"
     CDS             complement(67034..67729)
                     /gene="flgD"
                     /locus_tag="ACHKFK_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO9050767.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTALNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(67741..68145)
                     /gene="flgC"
                     /locus_tag="ACHKFK_000340"
     CDS             complement(67741..68145)
                     /gene="flgC"
                     /locus_tag="ACHKFK_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO9050768.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(68149..68565)
                     /gene="flgB"
                     /locus_tag="ACHKFK_000341"
     CDS             complement(68149..68565)
                     /gene="flgB"
                     /locus_tag="ACHKFK_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO9050769.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTKHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSVLSGQIKGMMNVLQSGN"
     gene            68720..69379
                     /gene="flgA"
                     /locus_tag="ACHKFK_000342"
     CDS             68720..69379
                     /gene="flgA"
                     /locus_tag="ACHKFK_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO9050770.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCDQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEASNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMISGQVVSGVVDADGNILIN
                     L"
     gene            69455..69748
                     /gene="flgM"
                     /locus_tag="ACHKFK_000343"
     CDS             69455..69748
                     /gene="flgM"
                     /locus_tag="ACHKFK_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO9050771.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            69753..70169
                     /gene="flgN"
                     /locus_tag="ACHKFK_000344"
     CDS             69753..70169
                     /gene="flgN"
                     /locus_tag="ACHKFK_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO9050772.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITGKTQQLRQLNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGSKKISI"
     gene            complement(70209..71744)
                     /gene="murJ"
                     /locus_tag="ACHKFK_000345"
     CDS             complement(70209..71744)
                     /gene="murJ"
                     /locus_tag="ACHKFK_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO9050773.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(71854..72777)
                     /gene="yceM"
                     /locus_tag="ACHKFK_000346"
     CDS             complement(71854..72777)
                     /gene="yceM"
                     /locus_tag="ACHKFK_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9050774.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATASSLRMDKHRTNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGNATLESGTLLTNESGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(72779..73426)
                     /gene="yceH"
                     /locus_tag="ACHKFK_000347"
     CDS             complement(72779..73426)
                     /gene="yceH"
                     /locus_tag="ACHKFK_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO9050775.1"
                     /translation="MKYQLTVMEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVENPPAVTDMSNAADGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(73437..74021)
                     /gene="rimJ"
                     /locus_tag="ACHKFK_000348"
     CDS             complement(73437..74021)
                     /gene="rimJ"
                     /locus_tag="ACHKFK_000348"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9050776.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVYDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            74257..75465
                     /gene="mdtH"
                     /locus_tag="ACHKFK_000349"
     CDS             74257..75465
                     /gene="mdtH"
                     /locus_tag="ACHKFK_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO9050777.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            75529..76176
                     /gene="grxB"
                     /locus_tag="ACHKFK_000350"
     CDS             75529..76176
                     /gene="grxB"
                     /locus_tag="ACHKFK_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO9050778.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            76310..76870
                     /gene="yceB"
                     /locus_tag="ACHKFK_000351"
     CDS             76310..76870
                     /gene="yceB"
                     /locus_tag="ACHKFK_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9050779.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     NGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            76976..78022
                     /gene="pyrC"
                     /locus_tag="ACHKFK_000352"
     CDS             76976..78022
                     /gene="pyrC"
                     /locus_tag="ACHKFK_000352"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO9050780.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NQAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELDRGFNEGVFTAA
                     KLYPANATTNSSHGVTSVDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            78096..78341
                     /gene="dinI"
                     /locus_tag="ACHKFK_000353"
     CDS             78096..78341
                     /gene="dinI"
                     /locus_tag="ACHKFK_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO9050781.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            78631..78885
                     /gene="bssS"
                     /locus_tag="ACHKFK_000354"
     CDS             78631..78885
                     /gene="bssS"
                     /locus_tag="ACHKFK_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO9050782.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            79000..80118
                     /gene="solA"
                     /locus_tag="ACHKFK_000355"
     CDS             79000..80118
                     /gene="solA"
                     /locus_tag="ACHKFK_000355"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO9050783.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQMLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETADGVYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            80166..80279
                     /locus_tag="ACHKFK_000356"
     CDS             80166..80279
                     /locus_tag="ACHKFK_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO9050784.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT        19755 a        21209 c        19987 g        19396 t
ORIGIN      
        1 gcctgctctc ccgatggttt atgcagtgac ggcaatggcc ttgagctgaa atgcccgttt
       61 acctcccggg atttcatgaa gttccggctc ggtggtttcg aggccataaa gtcggcttac
      121 atggcccagg tgcagtacag catgtgggtg acgcgaaaag atgcctggta ctttgccaac
      181 tatgacccgc gtatgaagcg tgaaggactg cattatgtcg tggttgagcg ggatgaaaag
      241 tacatggcga gttttgacga gatggtgccg gagttcatcg aaaaaatgga cgaggcactg
      301 gctgaaattg gttttgtatt tggggagcaa tggcgatgac gcatcctcac gataatatcc
      361 gggtaggcgc gatcactttc gtctactccg ttacaaagcg aggctgggta tttcccggcc
      421 tttctgttat ccaaaatcca ctgaaagccc agcggctggc tgaggagata aataataaac
      481 gaggggctgt atgcacaaag catctcccgt tgagttaaga acgagtatcg agatggcaca
      541 tagcctcgct caaattggag tcaggtttgt gccaatacca gtagaaacag acgaagaatt
      601 tcatacgtta accacatccc tttcacaaaa gctggaaatg atggtggcga aagcagaagc
      661 agatgagaga gaccaggtat gacaaccact gaatgcattt ttctggcagc gggcttcata
      721 ttctgtgtgc ttatgcttgc cgacatggga cttgttcaat gacacctcag caagaaaacg
      781 cccttcgcag tattgcccgt caggctaatt atgaaatcaa aaaagccaga cagcagtttc
      841 cggataaaaa cgtcgatgac atttgccgta gcgtactgaa gaagcaccgc gaaacggtaa
      901 cgctgatggg attcacaccg actcatttaa gcctggcgat cggcatgtta aacggcgtct
      961 ttaaggaacg gtgaacatga aaaacaaaat catcatggag ctacaggctc cttttttatt
     1021 attcgcattc accctcaagc gtattaacca acaattcagg gattaatgaa agatggcaga
     1081 catcattgat tcagcatcag aaattgaaga attacagcgc aacacagcaa taaaaatgcg
     1141 ccgcctgaac caccaggcta tatctgccac tcattgttgt gagtgtggcg atccgataga
     1201 tgaacgaaga cgcctggccg ttcagggttg tcggacttgt gcaagttgcc aggaggagat
     1261 cgaacttaag aacaaacaat ggggactgtg atggcctcaa agcagcaaat ttcaacatcg
     1321 tccaactgag gtgtaaaaat gttcagaatc atttttccta acacctggta cgtcgaccac
     1381 cacggcactc cctgcaaaat cctgcgttct acccacaaca aagttcacta catccgaaaa
     1441 ggcagaacat gtatcgccag catgttccgc tttaatcatg actttgaacc tgtgaataaa
     1501 gctgatgcag atcggatagc agaagagatc gaaacggcag aacacattaa gaagttacgt
     1561 gccatacgca ggaaatagaa aaattgataa attcaatact gcatttctca gcattaaatt
     1621 tatctctatg accagtcaag agatgtacct gccatgagct taatatcatg tcagatatat
     1681 cggtcacaaa ctccctcagc agctaagagg aggacaaatg tctcgactaa tcactttaca
     1741 ggactgggct aaagaagaat ttggggactt agcaccaagt gagcgagttc tgaaaaaata
     1801 cgcgcaaggg aaaatgatgg ccccacccgc tataaaagtt ggtcgctact ggatgattga
     1861 ccgaaattcc cgttttgtag gaacgcttgc agaaccgcaa ctcccaataa acgcaaaccc
     1921 aaaactccaa cggataatcg ctgatggctg ctagaccccg atctcacaaa atctctatac
     1981 ccaatttata ttgcaaatta gataagcgaa ccggaaaggt atattggcaa tacaaacatc
     2041 cactatccgg tcgttttcat agcttaggaa ctgatgagaa tgaagcaaaa caagttgcta
     2101 ctgaagcaaa taccattatt gctgaacaac gtacccgaca aatattaagc gtcaatgagc
     2161 gtctggaaag aatgaaaggc aggcgctcag acattacggt gacagaatgg cttgataaat
     2221 atatttctat ccaggaggac aggctgcaac ataatgaact aagacccaac tcctatcggc
     2281 aaaaaggcaa acccattcgt cttttccgtg agcattgtgg aatgcaacac ctcaaggata
     2341 ttaccgcact tgatattgcc gaaataattg atgctgtaaa ggctgaaggt cataacagga
     2401 tggcgcaagt cgtgagaatg gtgttaatcg acgtcttcaa agaagcacaa cacgcaggac
     2461 atgttccgcc aggatttaac ccagcgcagg caacaaaaca accgcgaaat cgagtaaacc
     2521 gccaaagatt atcactgccc gaatggcagg caatatttga cagcgtaagc agacggcagc
     2581 cctatttaaa atgcgggatg ctacttgctc ttgtcactgg acaacgttta ggcgatatct
     2641 gcaatttgaa attctctgat atctaggacg acatgttgca cattactcag gaaaaaaccg
     2701 gttcaaaact tgctattccg cttaacctga aatgcgatgc tctgaatatt acccttcgtg
     2761 aagttatatc tcagtgcagg gatgctgttg ttagtaaata tctggtccat taccgtcaca
     2821 caacctctca agcaaacaga ggagaccagg tttctgcgaa tactctgaca acggctttta
     2881 aaaaggccag ggaaaaatgt ggcataaaat gggagcaagg aactgcgccc acatttcatg
     2941 agcagcgatc tctgtcagaa cggttatatc gggaacaggg tctggatacg caaaagttgt
     3001 taggccataa atccagaaaa atgaccgacc gatacaatga tgatcgtggt aaagactgga
     3061 ttatcgtaga tatcaaaaca gcatagaaaa tagccagttt tggggaaggg ttttggggaa
     3121 agttttgggg aagattttac atcatcataa aacaacgggc gtgttatacg cccgttataa
     3181 tatttaacac atgtggtaat tacatatttt tgatgatcgc gtcaccaaac tcgctgcatt
     3241 tcagcagttt agcgccttcc atcagacgtt cgaagtcata ggttacggtc ttggcgttga
     3301 ttgcgccttc cataccttta acaatcaggt ctgcggcttc agtccagccc atatggcgca
     3361 gcatcatctc agcggagaga ataatagagc ctgggtttac tttgtcctgt ccggcatatt
     3421 tcggcgcagt accgtgggtg gcttcaaaca gggcgcattc gtcaccgatg tttgcgccag
     3481 gggcgatacc gataccacca acctgcgctg ccagggcgtc agaaatgtag tcaccgttca
     3541 ggttcataca ggcgataaca tcatattcag ccggacgcag caggatctgt tgcaggaatg
     3601 catcagcaat cacgtcttta atgacgatct ctttgccggt attcgggttt ttaactttca
     3661 gccacgggcc gccgtcgatc agttcaccgc caaattcttc acgcgccagc tggtagcccc
     3721 agtctttaaa cgcgccttcg gtgaacttca tgatgttgcc tttgtgcacc agggtcacag
     3781 agtcacgatc gttagcaatt gcgtattcga tcgctgcacg aaccagacgt ttggtgcctt
     3841 cttcagaaca cggcttaata ccgatgccgc aatgttccgg gaagcgaatt ttcttcacgc
     3901 ccatctcttc acgcaggaat ttaatcactt tctcggcgtc ggcagagtca gctttccatt
     3961 cgatacccgc ataaatgtct tccgagtttt cacggaagat aaccatatcg gtcagttcag
     4021 ggtgtttaac cgggcttgga gtgccctgat agtaacgtac cggacgcagg cagatgtaga
     4081 gatccagttc ctggcgcagg gcaacgttca gagagcgaat accgccacca accggagtgg
     4141 tcagcgggcc tttaatggca acgcgatatt cacgaatcag atcaagggtt tcagcaggca
     4201 accagacgtc ctgaccataa acctgtgtgg atttttcacc ggtgtaaatt tccatccagg
     4261 agattttacg ctcgccttta taggctttct cgactgcagc gtcgaccact ttcagcatgg
     4321 ctggggttac atctacaccg attccatcac cttcaatgta agggataatc ggattttcag
     4381 gaacgttgag tttgccgttt tgcagggtga tcttcttgcc ttgtgccgga acaactactt
     4441 tactttccat tcacctctcc ttcgagcgct actggtttgc tcgtctacca ccacgttgca
     4501 tatgcgtttg cgtcctgcga tacggatgct ttagagcaat tttttgttaa tgatttgtaa
     4561 ttggcttgtc aatactaccc tattgtttgc cgtcatgaaa gatgcgcgtt attaggctat
     4621 aatgcggcaa ttcataatct ctgaaaatac catgcaaaaa acttctttta gaaatcacca
     4681 ggttaagcga ttcagctcgc aacgttctac caggcgtaaa cctgacaacc agcccacgcg
     4741 tgtgatcctg ttcaataaac cctacgatgt tcttccgcag ttcaccgatg aagccggacg
     4801 caaaacatta aaagaattca tcccggttca gggggtgtat gcggcaggtc gtctcgaccg
     4861 cgacagcgaa gggttactgg tgctgaccaa taacggcgct ttgcaggcgc gtttaaccca
     4921 gccgggtaaa cgtaccggga aaatctatta tgtacaggta gaaggtattc ccacacaaga
     4981 cgcccttgaa gccttgcgca atggtgtaac cttaaatgat ggccctaccc tgcccgcagg
     5041 cgcggagctg gttgaagaac ccgcgtggtt atggccgcgg aatccaccaa ttcgtgagcg
     5101 caaaagtatt cccaccagct ggctgaagat caccttatat gaaggacgta atcgccaggt
     5161 gcgccgcatg accgcccatg ttggtttccc cacgctgcga ctgattcgct atgcgatggg
     5221 gaattactct ttggataatc ttgccaatgg cgaatggcga gacgcgacag attaaggatg
     5281 caaaatgttt aaaccgcacg ttaccgttgc ctgcgtggtg cacgcagaag gcaaattttt
     5341 agtcgttgaa gagacgatta atggtaaagc gttatggaac cagcccgccg ggcatctgga
     5401 agcagatgaa accttagtgg aagccgccgc ccgtgagctg tgggaagaaa ccggcatcag
     5461 cgcgcaaccg caacatttta tccgcatgca tcagtggatt gcgcctgata aaacgccgtt
     5521 tttgcgtttc ctctttgcca ttgagcttga gcaaatatgc ccgacgcagc ctcatgacag
     5581 cgatatcgac tgctgccgtt gggtcagcgc cgaagaaatt ttacaggcgt caaatcttcg
     5641 ttcgccgctg gtggcggaaa gtattcgttg ttatcaaagc gggcaacgct atccgctgga
     5701 gatgattggc gattttaact ggccttttac aaagggtgtc atctaaaagg ctgcgtgata
     5761 gaatacgccg ccttgaagtt caatgtcgtg agtgatccaa tgtctgaaac cgcaaaaaaa
     5821 gtaatcgtcg gcatgtccgg cggtgtcgat tcctccgttt ctgcctggct gttgcaacaa
     5881 cagggatatc aggtcgaagg cctgtttatg aagaactggg aagaagacga cggtgaggaa
     5941 tattgcacag cggcagcgga tctggctgat gcccaggctg tctgcgacaa gctcggcatt
     6001 gaactgcaca ccgttaactt tgctgccgag tactgggaca acgtcttcga actgttcctt
     6061 gccgaatata aagccggtcg cacgccaaat ccggatattc tgtgcaacaa agagatcaaa
     6121 tttaaagctt tcctcgaatt tgccgccgaa gatttaggtg ccgattatat cgctaccggt
     6181 cattacgtac gtcgggcaga tgtcgatggc aagagccgcc tgctgcgtgg cctggacagc
     6241 aataaagacc agagctactt cctttatacg ctcagccatg agcaaattgc gcaaagcctg
     6301 ttcccggtcg gcgaactgga aaaaccgcag gtgcgtaaga ttgctgaaga tcttggtctg
     6361 gtcaccgcga agaaaaaaga ttctaccggt atctgcttta tcggcgagcg taaattccgc
     6421 gagttcctgg gccgttatct cccggcgcaa ccgggcaaaa tcattaccgt cgatggcgat
     6481 gaaattggtg agcaccaggg gctgatgtat cacaccctcg gtcagcgtaa aggtctgggt
     6541 atcggtggca ccaaagacgg caccgaagaa ccgtggtatg tggtcgataa agacgtcgaa
     6601 aacaacattc tgattgttgc gcaaggccat gaacacccgc gtctgatgtc tgtcggtctg
     6661 attgcccagc agctgcactg ggtcgatcgc gagccgttca ccggtactat gcgttgcaca
     6721 gtaaaaaccc gctatcgcca gaccgacatc ccgtgcaccg tcaaggcgct ggacgctgat
     6781 cgcattgaag tgattttcga tgagccggtt gccgccgtga cgccgggcca gtctgccgtt
     6841 ttctataacg gtgaagtgtg cctcggtggc ggtattattg agcagcgtct gccgctgccg
     6901 gtctgattat tatctttact taaaacaagg aagcagtgaa cgtggcaaag aattactatg
     6961 acatcaccct cgcccttgcc ggtatttgtc agtcggcacg cctggtgcaa caactcgctc
     7021 accaggggca ttgtgatggc gatgcgctac acgtctcact caacagtatt atcgatatga
     7081 accccagctc aacgctggcg gtttttggcg gtagcgaagc caacctgcgc gtcgggctgg
     7141 aaaccttgct cggcgtactc aatgccagca gtcgccaggg cttaaacgcc gaattaaccc
     7201 gctacacact cagtctgatg gtgcttgagc gcaaactctc ctcagcgaaa ggtacgctcg
     7261 acactctggg caaccggatc aacggcctgc aacgccagct cgaacacttc gatttacagt
     7321 ccgaaacgct gatgagcgcg atggctgcca tctatgttga tgttatcagc ccgcttggcc
     7381 cgcgcattca ggtcactggt tcccctgctg tactgcaaag cccacaggtg caggcgaaag
     7441 ttcgcgcaac cctgctggca ggcattcgcg ccgccgtgct ctggcaccag gtcggcggcg
     7501 gtcgtctgca actgatgttt tctcgtaatc gcctgaccac tcaggcaaaa caaattcttg
     7561 ctcatttaac cccggagttg tgatctatgg aattatcctc actgaccgcc gtttcccctg
     7621 tcgatggacg ctacggcgat aaagtcagcg cgctgcgcgg gattttcagc gaatacggtt
     7681 tgctgaaatt ccgtgtacaa gttgaagtac gttggctgca aaaactggcc gcgcacgcag
     7741 cgatcaagga agttcctgct tttgctgccg acgcaatcgg ttaccttgat gcaattgtcg
     7801 ccagtttcag cgaagaagat gcagcacgca tcaaaaccat cgagcgtacc actaaccacg
     7861 acgttaaagc ggttgagtat ttcctgaaag aaaaagtggc ggagatcccg gaactgcacg
     7921 cggtttctga attcatccac tttgcctgta cttcggaaga tatcaataac ctctcccacg
     7981 cattaatgct gaaaaccgcg cgtgatgaag tgatcctgcc gtactggcgt caactgattg
     8041 atggcattaa agatctcgcc gttcagtatc gcgatatccc gctgctgtcc cgtacccacg
     8101 gtcagccagc cacgccgtca accatcggta aagagatggc aaacgtcgcc taccgtatgg
     8161 agcgccagta ccgccagctt aatcaggtgg agatcctcgg caaaatcaac ggcgctgtcg
     8221 gtaactacaa cgcccacatc gccgcttacc cggaagttga ctggcatcag ttcagcgaag
     8281 agttcgtcac ctcgctgggt attcagtgga acccgtacac cacccagatc gaaccgcacg
     8341 actacattgc cgaactgttt gattgcgttg cgcgcttcaa caccattctg atcgactttg
     8401 accgtgacgt ctggggttat atcgccctta accactttaa acagaaaacc attgctggtg
     8461 agattggttc ttccaccatg ccgcataaag ttaacccgat cgacttcgaa aactccgaag
     8521 ggaacctggg cctttccaac gcggtattgc agcacctggc aagcaaactg ccagtttccc
     8581 gctggcagcg tgacctgacc gactccaccg tgctgcgtaa cctcggcgtg ggtatcggtt
     8641 atgcgctgat tgcgtatcaa tccaccctga aaggcgtaag caaactggaa gtgaaccgtg
     8701 accatctgct ggaggagctg gatcacaact gggaagtgct ggctgagcca atccagacag
     8761 ttatgcgtcg ctatggcatc gaaaaaccgt acgagaagct gaaagagctg actcgcggta
     8821 agcgcgttga cgccgaaggc atgaagcagt ttatcgacgg tctggcgttg ccagaagaag
     8881 agaaagcccg cctgaaagcg atgacgccgg caaactatat tggtcgcgcc atcaccatgg
     8941 ttgatgagct gaaataagcc tcaaatcagt gccggatggc gatgctgtcc ggcctgctta
     9001 ttaagattat ccactttttg ttttttcact ttacctcccc ttcccgctgg tttatttaat
     9061 gtttaccccc ataaccacat aatcgcgtta cactatttta ataattaaga cagggagaaa
     9121 taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccat cttaaagttc
     9181 agattcagga tgctggtcat caggtcgatg acgcagaaga tgccaaagaa gccgattatt
     9241 atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcct gacgaggatg
     9301 gtctgtcact gattcgccgc tggcgtagca atgatgtttc actgccgatt ctggtattaa
     9361 ccgcccgtga gagctggcag gacaaagtcg aagtattaag tgccggtgct gatgattacg
     9421 tgactaaacc gtttcatagt gaagaggtga tggcgcgaat gcaggcatta atgcggcgta
     9481 atagcggtct ggcttcacag gtcatttcgc taccaccgtt tcaggttgat ctctcccgcc
     9541 gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac accattatgg
     9601 aaacgttgat acgcaataat ggcaaagtgg tcagcaaaga ttcgttaatg ctacaactct
     9661 acccggatgc ggagctgcgg gaaagtcata ccatagatgt actgatggga cgtctgcgca
     9721 aaaaaataca ggcacaatat ccgcaagaag tgattaccac cgttcgcggc cagggctatc
     9781 tgttcgaatt gcgctgatga aaaaattact gcatcttttt ttcccgctct cgctgcgggt
     9841 acgttttctg ttagcaacgg cagcagtagt attggtgctt tcgcttgcct acggaatggt
     9901 tgcgctgatc ggttatagcg tcagtttcga taaaaccacg tttcggctgt tacgtggcga
     9961 gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg tcgagttacc
    10021 cgaaaatatc gacaagcaaa gccccacgat gacactaatt tatgatgaga acgggcagct
    10081 tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcctg actggctgaa
    10141 atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct tgctgagtgg
    10201 agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg acgcggagat
    10261 gacccactcg gtggcggtaa acgtctaccc ggcaacatcg cggatgccaa agttaaccat
    10321 tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct ggagctggtt
    10381 tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg tcgccgcctg
    10441 gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg aagaacataa
    10501 ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac gaaacctgaa
    10561 ccgactgtta aaaagtgaac gcgaacgtta cgacaaatat cgtacaacgc tcaccgacct
    10621 gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt ctctgcgtag
    10681 tgagaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca gccgcatttc
    10741 acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat tgcttagccg
    10801 cgagctgcat ccggtcgcgc cactgttgga caatctcacc tcggcgctga acaaagtgta
    10861 tcaacgcaaa ggggtcaata tctctctcga tatctcgcca gaaatcagct ttgttggtga
    10921 gcagaatgat tttgtcgaag tgatgggcaa cgtgctggat aacgcctgta aatattgcct
    10981 cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg tggtcgagga
    11041 tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg gtcaacgggt
    11101 tgatacttta cgccctgggc aaggtgtggg gctggcggta gcccgcgaaa tcaccgagca
    11161 atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga tggaagtgat
    11221 ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtccatact tcacgcatta
    11281 cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta acatggaata
    11341 ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac gcccggtggt
    11401 gttaaaacgc ggctttaata attttattga cccaatctct ccagacgagt tggcgggtct
    11461 ggcgatggaa agcgaagtcg acagtcgact ggtcagtcat caggatggca aatggcaggt
    11521 cagccacggt ccattcgaaa gctacgatca tctcggtgaa actaactggt cattgttagt
    11581 gcaagcagtg aaccactggc atgagccgac cgccgcgcta atgcgaccgt tccgtgaact
    11641 accggactgg cgtattgatg atctgatgat ctctttttct gtaccgggcg gcggcgtcgg
    11701 cccgcatctc gatcagtacg acgtgtttat cattcagggt accggtcgtc gtcgctggcg
    11761 agtgggcgaa aagctgcaaa tgaaacagca ctgcccgcac ccggacctgt tacaggtcga
    11821 tccgttcgaa gccatcatcg atgaagagct ggagcctggc gatattcttt atattccgcc
    11881 aggattcccg catgaaggct acgcgctgga aaatgcgatg aactattccg tgggttttcg
    11941 cgcgccaaat acgcgggaac tgattagcgg atttgccgat tatgtgctgc aacgtgaact
    12001 gggcggcaac tactacagcg atcctgatgt tccacctcgc gctcatcctg cggacgttct
    12061 gccgcaagag atggataaac tgcgtgagat gatgctcgaa ttgatcaacc agccggaaca
    12121 ctttaaacaa tggtttggcg agtttatatc ccagtcacgt catgaactgg atatcgcgcc
    12181 gccagagccg ccttatcagc cggatgaaat ctacgatgcg ctgaaacaag gtgatgtgct
    12241 ggtgcgcctg gggggtctgc gcgtattgcg cattggcgac gacgtgtatg ccaatggtga
    12301 gaagatcgat tccccgcacc gtccggcact ggatgcgctc gccagcaaca ttgcgctgac
    12361 cgcggagaat tttggcgatg cgctggaaga tccgtcattc ctcgcgatgc tcgcggcgct
    12421 ggtcaatagc ggatactggt tcttcgaagg gtaagtttgg atttaagccg gatgcggcat
    12481 cacatgccgc atcccttttc gcttacttcc gttgcgccgt caactcggca atacggacga
    12541 tcacctgcac cgctttttcc ataccttcca gagtcacaaa ctcatgctta ccatgatagt
    12601 tgtaaccgcc agtgaacagg ttcgggcacg gtaatcccat aaacgacaac tgcgcgccgt
    12661 cggtaccgcc gcggatcggt ttcagttccg gttcaatatc gcagtcacgc atcgcctgct
    12721 gggcgatatc gagaatatgc ggatgctcaa ccactttctc gcgcatattg tagtaactgt
    12781 cttcaatcac cagttcaatg tagcaatcag gatgtaaccc tttgcccact tttttggcga
    12841 tctccatcat tttacgttta cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt
    12901 agtgcatatc ggcccgttca acggtgcctt tcatgttcgc caggtgatag aaaccttcat
    12961 agccttctgt catttccggg ctttcatccg ccggaacttc cgcatgaata cgtgccgcca
    13021 gcgacagcgc atttaccatc actcctttcg ccgtgcccgg gtgaacattg ttaccgacaa
    13081 ttttgatatt gaccgacgcg gcgttgaagt tttcaaactc cagttcacct acgccaccac
    13141 catcaacggt gtaagcccag cgggcatcga aggcatcaac atcaaaatgt ttcgcccctt
    13201 tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc accatgcgga atgttttttt
    13261 gttgtaatac cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca tcggcaccta
    13321 acaaggtttt accatcggtg gtaatcagcg tctgacccag tagctgatgc agcaccggga
    13381 acataaccgg tgataaaact tcatcgccga tacccagcgc aatatcgcca ccgcgatagt
    13441 tttcaacaat ttgcggattc acatttttgc cgctgcaatc cggtgaggta tccacatgag
    13501 aaataaagcc aatcgccggg atatcgccag ggacgttagc cggtaacgtc gccatcaaag
    13561 tgcccttctc acttaaggtc acattgataa gccccatctc ttcgagctgc tctttcagca
    13621 gatgcaataa cttccattgg ccttccgtgc tgggaacctg tctcacccct gcctttgatt
    13681 gggtatccag agacacgtag ttcaaaaatc gctcaagtag tttatccatg tagtcaccct
    13741 cactttttgt gacaacatta ttaagaagca agaaaagaca aatattgcgt caggtcactt
    13801 ttagccctgc aagcgggaat atttatcagc attaccttaa tgaatataaa gctaagccag
    13861 taattcacaa caaagattgg cgattcaagc ggtttgccgt agaattaccg ccctcattaa
    13921 gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa gccatccgtt gcgtttacat
    13981 gggacagagt aaaaaattga ataaacaacc gagttcgctt tcaccgctgg tgcaattggc
    14041 gggaattcgc aaatgctttg atggtaaaga ggtcattccc cagctggatc tgactatcaa
    14101 caatggcgag ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg
    14161 cctgattgca ggtctggaaa ctgttgattc cggacgcatc atgctggata acgaggacat
    14221 cacccacgtt ccggcggaaa accgctatgt gaacactgtt ttccaaagct acgcactttt
    14281 cccccacatg accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc
    14341 tgctgaaatt acgccccgcg tgatggaagc cctgcggatg gtgcagctgg aaaccttcgc
    14401 tcaacgcaaa ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca ttgctcgcgc
    14461 ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc tggattacaa
    14521 actgcgcaag caaatgcaga acgagctgaa agcgttacag cgtaagcttg gcattacatt
    14581 cgtgttcgta actcacgacc aggaagaagc acttaccatg tcggacagga tcgtggtgat
    14641 gcgcgatggt cgcattgagc aagacggcac gccgcgtgaa atctacgaag agccgaaaaa
    14701 cctgtttgtt gccgggttta ttggcgaaat caatatgttt aacgccactg tcatcgaacg
    14761 tctcgacgag cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa
    14821 cttcgccgtt gaaccggggc aaaaactgca tgttctgctg cgcccggaag acttacgtgt
    14881 tgaagagatt aacgacgaca accacgccga agggctgatt ggttacgttc gcgagcgtaa
    14941 ctacaaaggc atgacgctgg agtcggttgt tgagctggaa aatggcaaga tggtgatggt
    15001 cagcgaattc ttcaatgaag acgatcctga ctttgaccac tctctcgacc aaaaaatggc
    15061 cattaattgg gtagaaagct gggaggtcgt actggctgat gaagaacaca agtaagttcc
    15121 agaatgtagt gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt ctgcccaacc
    15181 tgatgatcat tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct
    15241 ttacgctgga taactacacg cgtctgctcg atccgctcta ttttgaagtg ctattgcact
    15301 cgctgaatat ggcgctgatc gccaccctcg cctgcctagt gctgggctat ccttttgcct
    15361 ggtttctggc gaagctgcca cacaaggtgc gtccgctgct gctgtttctg ctgattgttc
    15421 cgttctggac caactcattg attcgtattt acgggctgaa aattttcctc agcaccaaag
    15481 gctatctcaa cgagtttttg ctctggctgg gcgttatcga tacaccaatt cgcatcatgt
    15541 tcacgcctag tgcggtgatt atcggtctgg tttacattct gctgccgttt atggtgatgc
    15601 cgctgtactc cagtatcgaa aaactggata agccgttgct tgaggcggcg cgcgatctcg
    15661 gtgccagcaa gttgcagacc tttatccgca tcattatccc actgacgatg ccgggaatta
    15721 ttgccggatg tctgctggtg atgctgccag cgatgggcct gttctatgtg tccgacctga
    15781 tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa ggtacagttc ctcaatattc
    15841 gtgactggcc gtttggtgca gccaccagca ttacgctgac tatcgtaatg ggcctgatgc
    15901 tgctggttta ctggcgcgct tctcgtctgc tgaataagaa ggtggaactc gaatgatcgg
    15961 tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat
    16021 tattttgatt gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac
    16081 caccaaatgg tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc
    16141 actaacaatg gcggtgtttt cggcgacgtt tgctacgctt atcggttcac tgacggcagt
    16201 tgcgctgtac cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt
    16261 gatgatgtca ccagatatcg tgatggcgat ttcgctgctg gtgctgttta tgctgctggg
    16321 tattcagctt ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt
    16381 ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg cgaatgctgg aagcggcgaa
    16441 agatctcggt gccagtgaat ttatcattct gcgaaaaatc atcctgccac tggcaatgcc
    16501 agcggtggcg gcgggctggg tgctaagctt taccctgtcg atggacgatg tggtagtttc
    16561 gtcgtttgtc accggaccaa gctatgaaat tctgccgtta aaaatttatt cgatggtcaa
    16621 agtcggcgta tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt
    16681 gatggttatt gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt
    16741 taaatgaaaa aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc
    16801 gccgctcacg ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg
    16861 ccaggactgc ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac
    16921 gagtcgaacg aaaccatgta cgcgaagctg aaaacgtaca aagacggtgc ctatgacctg
    16981 gtggttcctt caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc
    17041 gacaagtcga agttaagcaa tttcagcaac ctcgatccag acatgctcaa caagccgttt
    17101 gacccgaata acgactattc cattccgtat atctggggtg caacggcgat tggcgttaac
    17161 ggtgatgcgg tggatccgaa atctgtcacc agctgggccg atctgtggaa gcctgagtac
    17221 aaaggcagcc tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgtaag
    17281 ctgggctact ccggtaacac caccgatccg aaagagattg aagctgcata caacgagctg
    17341 aaaaaactga tgccaaacgt cgcggcgttt aactccgata accccgctaa cccgtatatg
    17401 gaaggcgaag ttaacctcgg catgatctgg aacggttctg ctttcgttgc acgccaggcg
    17461 ggtacgccaa tagatgtggt atggccgaaa gaaggcggca ttttctggat ggacagcctg
    17521 gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg
    17581 cgcccggatg tggcaaaaca ggttgctgaa actatcggtt atccaacgcc aaaccttgcg
    17641 gcgcgtaagc tgttaagtcc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa
    17701 accattaaaa atggtgaatg gcagaatgac gtaggctccg ccagcagcat ttatgaagag
    17761 tattatcaga agctgaaagc aggacgttaa ttcagcaagc gggcggtggt attaccgccc
    17821 gttttttatt tagcaaggat gtggctaatg agccaggaca gtagagtttt ctttcgtata
    17881 ttcttcggta tcggtctggt actgatcctc atttctgttg ttatctttta taaccagttc
    17941 acctatagca aagacgcgat tcatactgaa ggcgtcattg ttgataccgt ctggcactcc
    18001 agtcattctc atcgcacggg taaaaatggt tcgtggtatc ccgtcgttgc ctttcgtccg
    18061 acgccagatt acacgctaat tttcaattcg agtattggca gcgattttta tgaagacagc
    18121 gaaggcgata aagttaatgt ttattattct ccaggacatc ctgaaaaggc tgagataaat
    18181 aacctgtggg ttaatttttt taaatggggg tttatcggca ttatgggcgt tatttttatc
    18241 gccgtcggtt tattaatcag tatgccctcc tctaaaaaga cacgcaggaa acggaagtcc
    18301 cgcccatgga aaaaatcatg atactgctca gtttactgat gttcctgcct ctgacggcaa
    18361 tcagtaagcc cttaattcca ataatgaaga ctctttttac agatgttacc ggtactgtac
    18421 cagatgctga agagatcgcg cataaagccg aactttttcg ccaacaaacc ggagttgcac
    18481 cgtttattgt ggttttaccc gacattaata atgaagccag tctcaggcaa aatggcaaag
    18541 cgatgctggc gcatgcggca tcttcaatga gtaatgtaaa aggaagtgtt ctgttactct
    18601 ttactacccg cgagccacgg ttaattatga taaccaacgg ccaggtcgaa agcagtatgg
    18661 acgataaaca tctcggcctt ctggtagaaa atcacacgct ggcttattta catgcggatc
    18721 tctggtatca gggtatcaat aatgcattgg ctgttttaca agcacagata ttaaaacaac
    18781 cgacgccacc actgacatat tatccacatc ccggacaaca gcatgaaaat gatccacccg
    18841 gcagtaccac gacattaggc ctttttgcct gggcagttgc attcatcgtc tttgctgcat
    18901 tttttaacta cacaacgcgc ctttattatg cgttaaaatt tgcagtggcg atggctgttg
    18961 ccaatatggg gtatcaggct ttatgtttgt atatcgataa cagctttgcg attaccagaa
    19021 tatcgccact ctgggcaggg ctgattggtg tctgtacgtt tattgccacg ttactatgga
    19081 cgagtaaacg gtaaaagagg cagtaaagtt cgcatgtatt atgccggatg cggcgcgaac
    19141 gccttatccg gcccacaaaa cccgcaaatt caattaattg cgccccttca caggcctgac
    19201 aagcgtagcg catcaggcaa tttttcccgc tttataatcc cttcagcaac ttttcaacaa
    19261 actctggcac cacctggctt gccggaccgt aatatttctc ggcaaattca ttaccaacct
    19321 gactcggttc aagattcagt tccacggtgt gcgcgccatg cagtttcgct tcgtgaacaa
    19381 acccagccgc cggataaaca tgccccgaag taccaatggc aatgaaaata tcggccatcg
    19441 acaacgccat ataaatttca tccataccga gtggcatttc gccaaaccac actacgtgcg
    19501 ggcgcaaggg tgccgggaac tggcaacaat ggcatttatc ttctggcgta acgtctccgg
    19561 tccagtcgag gacctggcca ctttgcgaac aacgtacttt cagcagttcc ccatgcatat
    19621 gaatgacatt ggtattacct gcgcgttcat gcagattgtc gatattctgc gtcaccagca
    19681 aaaagcgatc gccgagagca tcttgcagtt tagccagcgc aagatgtgca gcgttaggct
    19741 gaatttctgg ctgctgcagc tgtcgacgac gggcgttata aaacgtttgc accagttcag
    19801 gatcgcgatc aaaaccttcc ggcgttgcca catcttcaac ccgatgttct tcccacaggc
    19861 catctgcggc gcgaaaggta cgaatacctg attccgcaga aatccctgcc cctgtcagta
    19921 cgagtactct tggtttttcc attgcttccg gcaccacttt atctctgaaa aaaatacgct
    19981 gacgcaaacg ctcgcgcagg cgacgtttat ttttacgaaa acgacttaac cgatgacccc
    20041 gacgcgacag catagcaacc tctgttgttt aatcggttag atgtaggaag gccgcaccac
    20101 gcattccccc cgcatcaccg tgccgcgcgc gttcaatgcg cggaacacga gctacaggta
    20161 agagatgacg aggcaacctt tccgccagtt gcgttgtgat tgccgggaaa ttcgataagc
    20221 caccaccaat gacgaccagg tcagggtcaa caatggtcag gatatttccc agacaaaccg
    20281 ctaataaatc cagataacgc tcaacgtgcg cccttgcctg ctcatcgcct tgatcataaa
    20341 gcgcaatgat ctcaggagcc tgcaacggtt gatgataata gtgttgatac agccacgcaa
    20401 aaccgcgacc agacagataa ttttcaatgc agccatgctg accacagccg cagcggcgta
    20461 acgggaaatc cagccccatc atggttaacg catcaaccgg cagacgcata tggccaaact
    20521 cgccggtaat atagctttta ccggtaattg gtttaccgtt gaaaatcagc ccgccgccaa
    20581 cgccggtgcc gagaatcaac cccatcacca gtggatattg agtaaattca tcatcccatg
    20641 cttcagaaag ggcaaagcag ttggcatcgt tatcaaggcg tacatcgcga tcaagacgtg
    20701 cgctcaggtc ggcacgcagc ggtttaccgc tggcagcagg gacattggcg gcatacagcg
    20761 taccatcttc tgtttccggc attcccggaa taccgatgcc gacagaacct ttacagccaa
    20821 aacgccggtc agcttcggct accagctcgc acactgcatc taaaaatgcg tcatagctgt
    20881 cacgcggtgt cggcacccgc ttttcccact gcaactgccg accgctatca aacacgccta
    20941 gcgcaatttt tgttccacca atatcaaacc cgtaatacat tgccgctcct tgttttaatg
    21001 tactgccttt actggccgct aaggactcgc gcagggtcaa tattactggc gcgccgcgcc
    21061 ggataccaac ttgccaaaag actcagcaac aatgctgtga ccagtacgta gaagacgtcc
    21121 agccagtgca attccgatgg caggaagtca ataaaataga tatcgctgga gaggaactga
    21181 tgaccgatca gcttttcaat ccactcaata atcggggtaa gtttaagtga aaccaccacg
    21241 ccgataacca caccacacag gctgccgaac agccctgcca gcaatccata ccagacaaag
    21301 atggcgcgaa ttaaaccatc tttcgccccc agcgttctta atactgcgat atcgccactc
    21361 ttgtctttca ccgccatcac taaggtggag acgatgttga aacaggccac gccaatcacc
    21421 agcaccatcg ccagatacat aatggcgcgg atcatctgga tatcgcgata catatagccg
    21481 taagtaccaa tccagctttt aatataaaca tagctgtcgg tcacttcacc cgcatcgcgt
    21541 accagcttat tggcgttgaa aacatccgtc attttaaggg caatacctga cacgctggaa
    21601 cccatatcaa gatattgctg agcatcggcc agcgggatca tggcaaaact gtgatcgagt
    21661 tgaccactca actgcaaaat accggcaatg tgcaaacgca cacgttttgg ctgcatcagt
    21721 ttatgctcgg gattcgagtt ggggatcata atcgacaccc aatcgccctg cttcactttc
    21781 agcgcatccg ccacgccttt gccgatgata atttgctgtt cgcccgcttt aaaattgcgc
    21841 caggcatccc cctgaacaaa cgagggtaat gcgctcagac gctgttcctg ttgcgggtta
    21901 acgcccttca cctggattgc gcgcaaattc gcgccgctct ccaccagccc ggtgaaattg
    21961 atatacggcg cagcggcggc aatgcccggc actttctgca cgttatccag tgcttcctgc
    22021 cagttggtcc acggctgatc taccgcctca atttcaccgt gcggtactac cgccagaatg
    22081 cggttattca gttcgcgttc aaagccgttc atcgcgctta agccgacgat caataccgcc
    22141 acgcccaggg caatgccaat ggtagaaatg acggagatca acgacaccat gccgccgcgt
    22201 cgccgtccgc ggctaaaacg caggccaatc aataacgata aaggcatcgc cattactccg
    22261 cccccatcag gctcagttcc gccgtcagac gtccatcacg catttccagt tggcggctca
    22321 tacgtttcgc cagttgcagg tcgtgagtaa ccaccaggaa ggcggtgccc tgcaagcgat
    22381 tcaattcccc aagcaactgg aagatgctgt cggcgttacg tgcatcgagg ttaccagtag
    22441 gttcatccgc cagtaccagc cgcgggttat tgaccagcgc acgggcaatc gccacacgct
    22501 ggcgttcgcc gccagaaagt tcagatgggc ggtgattcgc acgatgctcc agccccaccg
    22561 cttttaacat ctccagcgca cggctgttga tttcagcggg ctttttcttg ccaatcaaca
    22621 gcggcatagc cacgttttcc agggcggtaa aatccggcag caggtggtga aactgataaa
    22681 taaatcccag cttctggttg cgcagttctg ctttcgccgc cgaagacagt ttgctcatcg
    22741 gttgaccgtt aaagatcaca tcgccggagg ttggcgtatc cagcccgcct aacaggtgca
    22801 gcaaggtgct tttaccggaa ccagagctgc cgacgatcgc catcatttca ccttcgccca
    22861 ccctgaaact gacattgtgc agtacatcgg tttgcacact gccttcctga tagcgtttgc
    22921 acaggttgtc gcattgcaac aggatcttat tcataacgta aagcctcagc gggttgagtg
    22981 gcggcagcgc gccaggaagg gtaaagcgta gacagcagcg cgatagccat cgccaccagc
    23041 gcaataacaa tgacctgtaa aggctcgata gcgaccggca gcgccgcgcc atcaagcagg
    23101 acgccgatta tcggcatcag attattcaac tggctggcaa gcagtgcgcc aagcgccgct
    23161 ccgaggatcg caccgataat cccagcgctg gccccttgca ccataaagac catcatgatt
    23221 tgtcgcggag tcagcccctg agtctgaaga atggccactt cgccctgctt ctccatcacc
    23281 atcagtccca gcgaggtaat aatgttaaac gccgcaacgg cgacaatcag gctcagcagc
    23341 aagcccatca tatttttttc catgcgtacg gcctggaaca gctcgccttt acggtcacgc
    23401 cagtcctgcc atttgctgcc ttcaggcagt ttttgctgac ttaatgagtc gactttcagc
    23461 ggctcatcca gccacaaacg ccagccggta atattgcctg ccggataacg catcagacgc
    23521 gaggcatcct caatattcac cagcatttca tagccatcga cttcactgtt ggcggcgaaa
    23581 gtgccaatca cattgaacag gcgctgactt ggaatacgcc ccatcggcgt gaactggcta
    23641 gcagatggca ccatcacgcg gatttgatca ccgcgattga cgcccaactg tgaggcaagc
    23701 tgttcaccga ggatgacatt atatttcccc ggctcgaggt cagtttgttt cacattgacc
    23761 agatacggcg ttagtggatc tttttgcgcc gggtcgatac ccagcatcac cccgaccgcc
    23821 acgctgcgcg cgctttgcag taccacatca ccggtagtaa taggtgcgac gcgattaacg
    23881 ccgtccagtt tgacggccgt ttccgggagt tgctgcgggt taagagagcc atgctcagaa
    23941 gagagaattg cctgtggcat caggccaagg atgttgtttt gcagctcgcg ctcaaagccg
    24001 ttcatcactg acaatactgt gaccagcgcc atcaccccga gggtaatgcc gatggtagaa
    24061 agccaggaga cgaaacgacc gaagcgatcc gctgcacgcc cacgcatgta acgcaggcca
    24121 ataaatagag cgacaggttg gtacatgaaa tccgtctggt tgctgtagca aagacacgag
    24181 tatataagcg aaagcaggat gggtaaacgt ctgaactgta agtgatcgtc agggctaatc
    24241 tgaaaagtac cggcaaatca atcggctaaa gtgcctcatt ttatcatcgg ccatgtggcc
    24301 tgatctttat ctgaaaaata accaaataca gacagatacg cattacacca cgcgactcgc
    24361 cccgcaggat caaccgcatg ttaaataaaa agagcgtgtt gtcctgatga aacaaaaaga
    24421 gctatggatt aaccagatca aagggttatg tatttgtctg gtggtgattt atcactcggt
    24481 cattaccttt tatccgcatc tgaccacttt ccagcatccg ttatcagaag tcctgagcaa
    24541 atgctggatc tatttcaatc tttaccttgc accctttcgt atgccggttt ttttctttat
    24601 ctctggctat ttgattcgcc gctatatcga cagcgtaccg tggggaaatt gtctcgataa
    24661 acgcatctgg aacatcttct gggtgctggc gctttggggc gtggtgcagt ggctggcgtt
    24721 aagtgcgctg aaccagtggc tggcacctga gcgcgattta agcaatgcct ccaatgccgc
    24781 ttatgccgat tccaccggtg agttcctgca cgggatgatc accgccagca ccagcttgtg
    24841 gtatctgtat gcgttaattg tctatttcgt gatatgtaaa atttttaacc gcctggccct
    24901 gccgttattt gttctgtttg tactgatgag tgtggctgtt aatttcgtac ccacaccgtg
    24961 gttgggaatg aacagtgtga tccgcaattt gccttattac agccttggcg catggtttgg
    25021 cacaacatta atgacctgtg ttaaagcggt accgttgcgc cgccatctgc tgatggcttc
    25081 tttgctgacc gttctggcgg tcggtgcctg gttgtttaat atctcgctgc tgttgtcgct
    25141 ggtatcgatt gtggtaatca tgaagctgtt ttatcagtac gagcagcgtt tcggtatgcg
    25201 ttcctccagc ctgctgaatg tgattggctc caacaccatt gctatctata ccacccatcg
    25261 cattctggta gaaatattca gcttaagcct gattccgcaa atgaacgcgg tacgctggtc
    25321 gccgcaagtc gaactgacgc tcctgctgat ttaccccttt gtcagtttgt tgatttgtac
    25381 tgttgcgggc ttgctggtaa gaaaactttc acagcgcgca ttcagcgatc tgttgttctc
    25441 cccgccttct ctgcccgcta ccgtcagtta ctcccgctaa gccatcgccc cagccggggc
    25501 gattcaattt gctaaaccat gccgattacg gataatgaac agcattgctt atcagagata
    25561 tgccctcata tgttgaggca tatcctaacg agaatctgac aaccgttatg cctgaacaat
    25621 atcgttatac gctgcccgtc aaagcgggtg agcagcgtct gctgggcgag ttaaccggcg
    25681 ctgcctgcgc gacgctggta gcggaaattg ccgaacgtca cgccggtccg gtggtactca
    25741 ttgcaccaga tatgcaaaat gctctgcgtt tgcatgatga aatcagccag ttcaccgatc
    25801 agatggtgat gaatctggcg gactgggaaa ccctccctta cgacagtttt tcgcctcatc
    25861 aggacattat ctcctcgcgt ctttccaccc tttaccagct accgaccatg cagcgtggcg
    25921 tactgattgt tccggtgaat acgcttatgc agcgcgtttg cccgcacagc tttctccacg
    25981 gtcatgcgct ggtgatgaaa aaaggtcagc gcctgtcacg agatgcatta cgaacccaac
    26041 tggacagcgc cggttatcgc catgttgacc aggtgatgga gcacggcgaa tacgccacgc
    26101 gcggcgcgtt gctggatctc ttcccgatgg ggagtgagct gccttatcgt cttgatttct
    26161 ttgatgatga aatcgacagc ctgcgggtgt ttgacgtcga tagccagcgc acgctggagg
    26221 aagtagaagc gatcaatctg ctgcccgcgc acgaatttcc gactgataaa gcggcaattg
    26281 aactgttccg cagccagtgg cgcgatacct tcgaagtgaa gcgcgatccg gaacatattt
    26341 accagcaagt gagtaaaggc acattacctg ccgggatcga gtactggcag ccgctattct
    26401 tcagcgaacc gctgccgcca ctgttcagtt atttccctgc caatacgctg ctgataaata
    26461 ctggcgatct ggaaaacagt gccgaacgtt tccaggctga cacgctcgcg cgttttgaga
    26521 atcgcggtgt cgacccaatg cgcccactgt tgccaccaca atcgctctgg ctgcgggtag
    26581 acgagctctt ttcagagctg aaaaactggc cgcgtgtgca gctaaaaact gaacatttac
    26641 cgacaaaagc agcgaatgcc aatttaggtt tccagaaact gccagacctg gccattcagg
    26701 cacaacaaaa agcgccgctg gatgcgctgc gtaagttcct cgagactttc gacggtccgg
    26761 tggtgttctc ggtagaaagt gaaggtcgcc gtgaagcgct gggtgaactg ctcgcgcgaa
    26821 ttaaaattgc tccgcaacgc attatgcgtc ttgatgaagc cagcaaccgt gggcgttatc
    26881 tgatgattgg cgctgccgaa catggttttg tcgatacgat gcgtaacctg gcgctgattt
    26941 gcgaaagcga tctgctcggt gaacgcgttg cccgtcgtcg tcaggattct cgccgcgcca
    27001 tcaaccccga tacactgatc cgtaaccttg cggagctgca tattggtcag ccggtggtcc
    27061 atctggagca cggcgtcggt cgttatgccg gaatgaccac gctcgaagct ggaggcatta
    27121 ctggcgagta tttgatgctc acctatgcca acgacgccaa actgtatgtt ccggtgtcgt
    27181 cactgcatct gattagccgt tacgcgggtg gcgcagaaga aaacgcacca ctgcataaac
    27241 ttggtggcga tgcctggtca cgcgcacggc agaaagcggc ggaaaaagtg cgtgatgtgg
    27301 cggcggaatt gctggatatc tacgcgcaac gcgccgccaa agagggcttc gcgtttaaac
    27361 acgatcgtga gcagtatcag ttattctgcg acagcttccc gtttgaaacc acgccggatc
    27421 aggctcaagc cattaatgcg gtgcttagcg acatgtgtca gccgctggca atggatcgtc
    27481 tggtgtgcgg cgatgttggc tttggtaaaa cagaagtggc gatgcgcgcc gctttcctgg
    27541 cggtagataa ccacaaacag gtggcggtgc tggtgcctac cacccttctc gcgcagcagc
    27601 attacgacaa cttccgcgac cgtttcgcca actggccggt acgtatcgaa atgatctccc
    27661 gtttccgcag cgccaaagag cagacgcaaa tccttgcgga agtggcggaa gggaaaatcg
    27721 atattctgat cggtacgcac aaactgctgc aaagtgacgt caagtttaaa gatttaggcc
    27781 tgctgattgt cgatgaagaa caccgcttcg gggtgcgtca taaagagcgc attaaagcga
    27841 tgcgcgcgaa cgtggatatt ctgacgctta ccgcaacgcc gatcccacgt acgctgaata
    27901 tggcaatgag cggaatgcgc gatctgtcga ttatcgccac gccgcccgcc cgtcgtctgg
    27961 cagttaaaac ctttgtccgc gagtatgaca gtctggtggt ccgggaggcg atcctgcgtg
    28021 aaattttgcg cggaggacag gtttattatc tctacaatga tgtggaaaac atccagaaag
    28081 ccgccgaacg gctggcagaa ctggtacctg aagcgcggat tgctatcggt cacgggcaaa
    28141 tgcgtgagcg cgaactggaa cgggtgatga atgatttcca tcatcaacgt ttcaacgtgc
    28201 tggtttgtac caccattatc gaaaccggga tcgacattcc taccgccaac accattatca
    28261 ttgaacgcgc ggatcacttc ggtctggcac agttgcacca gttgcgcggt cgcgtcgggc
    28321 gttctcacca ccaggcatat gcatggctac tgacgccgca tccaaaagcg atgactaccg
    28381 atgcgcaaaa acgtcttgaa gcgattgcct cgctggaaga tctcggtgca ggttttgcgc
    28441 tggcaacgca cgatctggag atccgcggcg caggtgaact gcttggcgaa gaacaaagcg
    28501 gctcaatgga aaccatcggt ttctcgctgt atatggagtt gctggaaaac gccgtcgatg
    28561 cgctgaaagc cggacgcgag ccgtcgctgg aagatctcac cagccagcaa acagaagtcg
    28621 agctgcggat gccgtcgcta ttgccagatg atttcatccc tgacgtgaac acgcgtctgt
    28681 cgttctacaa acgtattgcc agcgccaaaa cggaaaacga actggaagag atcaaagtcg
    28741 agcttatcga tcgcttcggc ctgctgccgg atccggcgcg taccctgctg gatattgccc
    28801 gtctgcgcca gcaagcgcag aaactgggga tcaggaagct ggaaggtaat gagaaaggcg
    28861 gcgtgatcga atttgccgag aagaatcacg ttaatccggc ctggttgatt ggtttgctgc
    28921 aaaaacagcc gcagcattac cgccttgatg gtccgacgcg tctgaagttt attcaggatt
    28981 tgagcgagcg gaaaacgcgt atcgaatggg tacgccagtt tatgcgtgaa ctggaagaga
    29041 acgcgatcgc ttaatggtaa atcgggggcg tttctgcgcc cccatctgca atatttacaa
    29101 attctttgca cttccctgca ttatccgaca ctgtcaccct ccataattca gacttacctt
    29161 tttattacaa taaccttata tttattatgg actttggtga tgatcaaaac gcgtttttct
    29221 cgctggctaa cgttttttac gttcgccgtt gccgtggcgc tggcgctacc ggcaaaagcc
    29281 aacacctggc cgctgccgtc agcgggcagt cgtctggttg gtgaaaacaa atttcatgtg
    29341 gtggaaaatg acggtggttc tctggaagcg atcgccaaaa aatataacgt cggctttctc
    29401 gctctgttac aggctaaccc cggcgttgat ccttacgtac cgcgcgcggg tagcgtgtta
    29461 acgatcccgt tgcaaaccct acttccagac gcgccgcgcg aaggcattgt gatcaacatt
    29521 gcggagctgc gtctctatta ttatccgccg ggaaaaaatt cggtaaccgt atatcccatc
    29581 ggcattggtc agttaggtgg tgacacgctg acaccgacaa tggtgaccac cgtttctgac
    29641 aagcgtgcaa acccaacctg gacgccaacg gcaaacattc gcgcgcggta taaggcacag
    29701 ggaattgagt tgcctgcggt agttccggct ggaccggata acccaatggg ccatcatgcg
    29761 attcgtctgg cggcctatgg cggcgtttat ttgcttcacg gtacgaacgc tgatttcggc
    29821 attggcatgc gggtaagttc tggctgtatt cgtctgcggg atgacgatat caaaacactc
    29881 tttagccagg tcaccccagg caccaaagtg aatatcatca acaccccgat taaagtctct
    29941 gccgaaccaa acggtgcgcg tctggttgaa gtacatcagc cgctgtcaga gaagattgat
    30001 gacgatccgc agctgctgcc aattacgctg aatagcgcaa tgcaatcatt taaagatgca
    30061 gcacaaactg acgctgaagt gatgcaacat gtgatggatg tccgttccgg gatgccggtg
    30121 gatgtccgcc gtcatcaagt gagcccacaa actctgtaaa gctcggataa aaaaatcccc
    30181 gcgacacggc atgtcgcggg gaaaaaaaca agcagtagca ggcattaatg agggttaatg
    30241 cttatttata aattactgct gttccatgga gggtattcgg accggttaca gaagtaatac
    30301 ggaaagattt tgcgcccatc tcatccgctt tttgcgccag ctgctcttcc agcgatccca
    30361 gatttgtccc cgcgttagca ctgatagtac cgactttttg ttggccttct ggcgttgact
    30421 gaacttcgac agccgcaaag ctggcaaatg acatggagct taaaatcgcc gcagcgatga
    30481 gggtttttac gtttttcata atagtggcct tatgcagatg aatgacgtcg caagcaatta
    30541 cttaacgatc gataactaaa tgatagatgt gatctggatc acatacaaga tatttttata
    30601 acaatcatta attaatttaa aaacctttaa atttcagtta aatagaaagc aattattttt
    30661 atggaatctg gactggtgaa cggaggtgat tatttttata ataacctttc attcacagtc
    30721 accaggtaca acggcatcat ggcaactgac tcaacacaat gtgtaaaaaa aagccgtggc
    30781 cgcccaaaag tgttcgacag ggatgccgcg cttgataagg ccatgaaatt gttctggcag
    30841 cacggttatg aagcgacttc tcttgcggac ctcgttgaag cgaccggagc taaagcgccc
    30901 acgctatacg cggaatttac caacaaagag gggttattcc gcgccgtact cgaccgctat
    30961 atcgatcgtt ttgccgctaa gcatgaagca cagctgtttt gtgaagagaa aagcgtggcg
    31021 tctgcgctgg ctgactattt tgctgccatc gccaactgct ttaccagcaa agacaccccg
    31081 gcgggctgct tcatgatcaa caactgcacc accctctccc cggattctgg agatatcgcc
    31141 aatacgctga aatcacgcca tgcgatgcaa gagcgcactt tgcagcagtt tttatgtcaa
    31201 cgacaagcgc gcggggaaat cccgacccac tgtgacgtga cgcatctggc agaattcctt
    31261 aattgtatta ttcaggggat gtcgatcagc gcacgcgaag gtgcatcgct ggaaaaactg
    31321 atgcagattg cccgaacgac tttgcgttta tggccggaac tgctgaaata ggtcgcaaaa
    31381 aaagcccctc agcttgagcg aatgaggggc taaattgcag agtacaacct tgttatgctt
    31441 tgttattcaa aatcagctgc ccgttgctat ctagcgggat ctgcgtaccc ggatcgcggt
    31501 ccattcggat tttgccctgc tgatcgccaa tcttataggt cacatcataa ccgagcattt
    31561 tttctgactt gtcatacacc gttttacaac gctgttgcgt agtcgtgtaa gtatcgcttt
    31621 cctggagaga gccctggatc tggttacctg catatccacc ccccagcgcc cccacaacag
    31681 tggcgacatc tttaccgcga ccaccaccaa actgatgccc aatcacgccg ccagcaacag
    31741 cgccgagcac cgacccggta atgcgatttt catcctgcac cggtcgacga tgggtcactg
    31801 taacgttgcg acactcctga cgcggtgttt taaccgtttc cttgattggg gttgcagaaa
    31861 caacctgagc gtattgcggg ccacgttcaa acacgttcag actggccact gccgctacgc
    31921 ccagcgcagc tgcgacacca atcccgatac ccgccaacat tgacttattc acgggaatac
    31981 ctccttcttt gctgttatgc caatcatgca agcgaaggag aaaagcagct atcagacaga
    32041 agatgaaaag cgcgggaatg gctggaaata cttagggatt cggaggagtc tgaaggaatg
    32101 cctgatgcga cgcttaacgc gtcttatcag gcctaccgct cgtaccattt gtccagggcg
    32161 gataaggcgt ttacgccgca tccgccagta caagccgatt aatgcaactt caaacgtgga
    32221 cggataacgc ggttaatact ccccaccagc atcattaatc cggttttaaa gtaaccatgc
    32281 agcgcaatct ggtgcattcg gtacagcgag atgtatacaa agcgcgcaat tcgtccttca
    32341 atcatcattg agccgcgcgt caggttaccc atcaggctac cgactgtgga aaagttcgac
    32401 agcgatacca gcgaaccgtg atctttatac tgataatttt tcagcggctt accgttcatc
    32461 tgcgccagaa tgttgttcat tgcgcaggtt gccatctgat gtgcggcctg agcgcgcggc
    32521 ggaacaaagc ccccttccgg acgcgggcat gacgcgcagt cgccaatagc gtatatgtct
    32581 ggatcgcgag tggtttgcag cgtcggttcc accaccagct ggttgatacg gttggtttca
    32641 agaccaccga tatctttcag gaagtctggc gctttgatcc cggctgccca caccatcaga
    32701 tcagcctcaa tatattcgcc atctttagtg tgcaggccgc cttcatcagc actggtgacc
    32761 atggtttgcg tcagcacgcg aacgcctagt ttcgttaact cactatgggc cgcagcagag
    32821 atacgtggcg gcaatgcagg caaaatacgt tcgcccgctt ctaccagcgt tacgttcagg
    32881 gcttcgttgg tcagaccttt ataaccgtaa ctgtgcagtt gcttgaccgc gttgtgcaat
    32941 tcagcggaga gttctacacc cgtcgcgccg ccgccgacaa tcgcaatgtt tactttgcca
    33001 ttcgcgccca ggttggcgga gtatttcagg aacagattca gcatctcctg gtggaagcga
    33061 cgcgcctggt gcgggttatc gaggaaaatg cagttctctt tgacacctgg tgtattgaaa
    33121 tcgttagagg tgctacccag cgccattacc agggtgtcat aggcgatttt acgttccgga
    33181 accagcagtt cacctttctc gtcgcgcagt tctgcaatag tgattgtttt cgcttcacga
    33241 tcaatatcaa ttacggaacc cagctggaac tggaaaccat gattgcgcgc atgggccaga
    33301 tagctcaacg catcgacgcc ttcatcaagc gagccagtcg ccacttcgtg cagcagcggt
    33361 ttccacaggt ggctgtggtt acgatcgacc agcgtaattt tggctttttt cttgcgtccc
    33421 agcttatgcc ccagctgtgt tgccatttcc agcccaccag caccgccgcc gacaatcaca
    33481 atttttttca atggcgtagt caacgtgacc cccttaaatt tattaaccaa ttgttaatta
    33541 atggttatta acatagctct taattaacaa caggttacag caatgaaaat gttcttcgct
    33601 attgagaata acatgaatgg tgcattggtc ataccaaaat tgatgttaat caagttttat
    33661 tgctgaaaag ataggcgact ttaccgggtg agatcaaaaa acagagtttg ttgtttatta
    33721 tttgagctga tttgtgagaa tgatatagag tgaggtgtgt atggagggcg ttcggtaggc
    33781 ctgataagag gcgcatcagg cattgatgct cgactgtcgg atgcggcgga acgccttatc
    33841 caacccacag aatccggagc gcatcaggta ccggcaaggt tacctgatgc gacgagggca
    33901 tttacccgag ggttttgaac gctttaatgc gctgtaaatg cggggagata ttcttgaatt
    33961 tgtgcgtctg ctcttcgtcc cagacaatct cgtaataatg atgcaactct tcagatgtcc
    34021 gctggctgtt aagcgcttca tcattacgcg acaaaatcac caggcaacga tcgcgattct
    34081 tctcacggaa gttggtcaca cacttagtgg caatatcggc atactcttcc gggcgatcaa
    34141 tcttcccttc catgttctca taagggaaca aattggggtt gaagatcacc tggcggatat
    34201 cacagagaaa accaatccgc tcagcccaat aaccgcccag accaacacca caaatgagcg
    34261 ggcgctcgtc gacgttcagt tgcaacattt tgtccacttc tttgagcaaa tgctgcatat
    34321 catgtttcgg atgccgcgta ctgtagctta ttagccgtac atccgggtca ataaactgca
    34381 attgtaagac tttctcgtgg ttacccggac tgttagagtc aaaaccgtgc aaatagataa
    34441 tcatcgcttc ctcacataag ccagggttaa tgacctgcct tctcttcctg ccagcgttca
    34501 tgtaactgat tcagacgggt gctgatcgct ttccagcgag ccgagtccat cagttcctgt
    34561 cgcgaaaatg aacctttatg atacaaacgt gtaacacgtt ctgccttgat cggtgacaga
    34621 ttatctaaca cgctgacggc ccctttacga ttattgcaga ccaggatcat atcgcaacct
    34681 gcatccagtg atgcctgtcc acgctcggca taactgccca taatcgctgc gccttccatc
    34741 gataaatcgt cagagaaaat cacgccgtca aaacccaatt cctgacgcaa aacggttttc
    34801 agccagtagg gagaaccgct cgccggacgc ggatcaacat cactgtagat cacatgcgca
    34861 ggcataatgg cgtcgagttt attttcgcgg attaaggaac tgaagaccga catatcttta
    34921 gcgcgaatct ccgcttgcgg acgtgggtca cacggtgttt ctttgtgtga gtctgccgtt
    34981 actgcaccgt gacctgggaa gtgtttcccg gtagttttca ttcccgcttc atgcatacca
    35041 tcaataaaac ggctggcaat tgccagggct ttttgtggat cggcatgata agaacgctcg
    35101 ccaatcgccg cgctgatatg cccgacgtcc agcactggcg caaagctgat atcaatatcc
    35161 atagcaatca tttcactggc catcaaccaa cctgcctctt gcgccagttt tccgccttct
    35221 tccattccca acagcgcagc gaatgattgt gccgctggca agcgggtaaa accttcacga
    35281 aaacgctgca cgcgtccacc ttcctgatct accgccacca ccagatgatt gcgcgatgct
    35341 gcgcggatct ggcgcaccag ttcacgtaac tgggcaggat catgatagtt tcgcgtaaag
    35401 agaatcagcc ctcccaccag cggatgcgcc agtatttcac gctcttccgc gtccagttcg
    35461 taaccttcga catccaacat tactggaccc acactgctct ccttattgtt ttattaatag
    35521 ctgccgccac gtgtcatcgg ccagcctgat aaattgttga tcgccggttt gtcgccagcg
    35581 gtactcaaac caccctgctt tgagcatcag cagccaggga aaccatcgcc tgacctgacg
    35641 ccataattgc gccggataaa tcttcgcgcg agtggcatag tcattgacca attgccgatg
    35701 ctggtcaata ttttccaccc acaccgccgc cagttccagc gcgatatcac catctccggc
    35761 atactcccag tcgatgagtt ttaaccctga cgcgctatgc actaaatttc cggcgtggac
    35821 atccatatgc aatggactta agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt
    35881 taacattcgc agccaaccca ctgtccgccg cgccggatcg ctttgctgcc agtacagttc
    35941 cagtaacggc aacagcgtta ttcgccagcc aaaacgcggt tgttgatgta gataatacag
    36001 caagcctgcc agttcgttgg tatccggcaa atacgttttt acctcgccgg gctgatagtc
    36061 gaccaccatc cagtcacgga gatataaatg cggcttcggt gcaatgcatg cgggtagttg
    36121 tgataaagcc cgatactggc gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg
    36181 acgcacaaca aaacgctggc cctgatgttc aatgagaaaa ctcccgccac taagcccact
    36241 attaaacgtc gtgacgggat gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt
    36301 gaggggatta ttgctgcgaa acggcacctt tacctgacca gataatttcg cccgtctgca
    36361 ccagcatcag ctgcatttgt agggtcggag cgttaacgtt gccagaggcg ctggagtaca
    36421 gcacgtaatg agcgccaaca ttgcgggcaa tgcctatggc tttactacgg gtgcctaaac
    36481 tgtcctgcgg cgataaacct aattgttgct tagccattga cagctgctgg gcggaaacca
    36541 gggtaaattt accgttatta gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat
    36601 tcaacgaacc gttagtacgg ttgttaacgc tatcaaccag caggacgcta cccgcagtca
    36661 cgccgtcagc cccaagcatc ttactgacca tcggctgcat tgcgccattc cagtcataat
    36721 gacgaatatg cggcgcaggc ggtgcagttt gatcttcgtg ctcaattggg cctggctgct
    36781 gcggtatcgt cggcaccgat ggcactgtgg ggacaggctg ttgtggctca gcaggttgtt
    36841 ccggcgctgg tttcacttct tctaccggtg caggttcacg ttgccccaca cacccggcaa
    36901 gaaacatcgc cagcgcggta atcaaggcgt agcgactcat ttttgtcatc aagattcacc
    36961 ccttacaaat atagataaag tctgactttg tgcgccccca gaaaattggc gctgccgtac
    37021 agcgtgaccg ccgaatgtgc gggaatggta acgctgcgtg gcctttccag aggatgcatc
    37081 tccagccctc tggcgtcata ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt
    37141 tcgttataaa gcgttgagga tgctgaaggt tgaatatcag atgtcgaaag gacgggcttt
    37201 tctgcactga tgcccgcagc caataaagat gactccatca ccagcgattg ctcatcattc
    37261 accggaattt ccggatgtga acgacagccc accagcacta acaacgccag agacatcagc
    37321 ccaaagcatc cttttctcat cgttacagac ctttatgcgt cagcattggt cccagcggac
    37381 ggccacccaa caaatgcata tggatgtggt aaacctcttg tccgccatgg cggttggtgt
    37441 tcatgatcag acgatagcca tcttcggcaa taccttcttg ctcagcaatt tttgccgcta
    37501 cggtgatcat gcgtcccagc gcctgctcat gctcagctga cacgtcgttc acagtcggaa
    37561 tgaggatatt cggaatgatc agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg
    37621 ccgttaccag atcatcctgg tagacgatat cggaggggat ctcacgacga ataattttgc
    37681 tgaatatagt ttcttctgcc acgacatttt cctttttcaa atagccatgc gttgcgccat
    37741 gctacgccgc gccacactgc gagtatagag tatggtcgag ttaaacctgc tctttcaacc
    37801 ttaactcacg atttcttaag caaaaaatcg ttttgctgcc gtatatatcg ccattattcc
    37861 catttctgcc tgcgaatgcg ctgaaaagac attctctgtt tggtttacac tccaaatatg
    37921 aatgcgtata tttctcattt gcatttacac acaaaaatta ttcgcacact aaatataacc
    37981 gctcatcacc ctgcttcggc atgagcctga cgccaacata aaccaagaga tttcaaatgc
    38041 tttcgacaca atttaacagg gataatcaac atcaagccat catcaaaccg tcactccttg
    38101 ccggttgcat agcactggca ctattacctt ccgccgcttt tgctgcacca gctactgaag
    38161 aaacggtgat tgttgagggt tcagccacag ccccagatga tggcgaaaat gattacaacg
    38221 taacgtctac ctctgcgggt acaaaaatgc agatgactca acgtgatatt cctcagtcgg
    38281 tcactattgt tagccagcag cggatggaag atcagcagtt acaaaggctg ggcgaagtga
    38341 tggaaaacac gctggggatc agcaaaagtc aggcggattc cgatcgtgct ctttattatt
    38401 cccgcggatt ccagatcgat aactatatgg ttgatggtat ccccacctat tttgaatcgc
    38461 gctggaatct gggcgacgca ctttctgata tggcactgtt tgaacgcgta gaagtagtgc
    38521 gtggcgcgac aggcctcatg accgggacgg gtaatccatc tgcggcaatt aatatggttc
    38581 gaaaacacgc gaccagtcgt gaatttaaag gcgatgtctc ggcggaatac ggtagctgga
    38641 acaaagaacg gtatgtggcg gatttacaaa gcccactcac cgaagacggt aaaatccgcg
    38701 cacgaattgt cggcggctac cagaataacg actcatggct ggaccgctac aacagtgaaa
    38761 agaccttctt ctccggcatt gtcgatgctg atttaggcga tcttacgatg ctttcagccg
    38821 gttacgaata tcagcgtatt gatgttaata gcccaaccta gggcggttta ccgcgctgga
    38881 atactgatgg cagcagcaac agttacgatc gcgcacgcag taccgcacct gactgggcgt
    38941 acaacgataa agagatcaac aaggtcttta tgaccctgaa gcagcggttt gctgatacct
    39001 ggcaagcgac gctgaatgcc acccactctg aagtcgaatt tgacagcaaa atgatgtatg
    39061 tcgatgccta tgtaaacaaa gcggatggta tgctggttgg gccatacagt aattatggcc
    39121 ctggttttga ttatgtcgga ggcaccggtt ggaacagcgg caaacgtaaa gttgatgcgc
    39181 tggatctgtt cgctgacggt agttatgaat tgtttggtcg tcagcacaat ttaatgtttg
    39241 gtggcagtta cagcaaacaa aacaatcgtt acttcagttc atgggccaac attttcccgg
    39301 atgaaattgg cagtttctac aactttaatg gcaatttccc acaaaccgac tggtcaccac
    39361 agagcctggc gcaggacgat accacacata tgaaatcgtt atatgctgcc actcgtgtca
    39421 cccttgccga tccgctgcat ctgatcctcg gcgcacgtta taccaactgg cgagttgata
    39481 cgctgactta cagcatggag aaaaaccaca ccacgcctta tgctggactg gtgtttgaca
    39541 tcaatgataa ctggtcgacc tacgccagct atacctctat tttccagccg caaatgatcg
    39601 tgacagttca ggcaaatatc tggctccaat caccggtaac aactacgagc tgggtctgaa
    39661 atcggactgg atgaatagcc gcctgaccac cacgttagcc atcttccgta ttgagcagga
    39721 taatgtcgct cagtccaccg gtacacctat ccccggcagc aacggcgaaa ccgcctataa
    39781 agcggtggat gggacagtca gtaaaggtgt ggaatttgaa ctcaacggcg caattaccga
    39841 caactggcag ttgacgtttg gcgcaacgcg ctatattgcc gaggataacg aaggaaacgc
    39901 cgttaatcct aatctgccac gcaccacggt taaaatgttc accagctatc ggttgcctgt
    39961 catgccaaag ttgacggtcg gcggtggtgt taactggcaa aatcgcgtgt ataccgacac
    40021 cgtgacaccg tatggcacct tccgcgccga gcaaggtagc tacgcgctgg tggatctctt
    40081 cacccgctac caggtgacga aaaacttctc gttacagggg aatgtcaata acctgttcga
    40141 caaaacctac gataccaacg tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag
    40201 cattaccggc acgtatcaat tctgaataac gcctgtaaaa aaggcagcca tctggctgcc
    40261 ttagtctccc caacgtctta cggattagtg gttacggatg tactcatcca tctcggtttt
    40321 caggttatcg gatttagtac cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc
    40381 cgctgcgccc agtttcttca ggccagcctg atccacttta gacacatcag caacgctgac
    40441 gcgcagacgg gtaatacatg cgtcgaggtt agtaatgttt tctttaccac caaatgcagc
    40501 aaccagagcc ggtgccattt cgctggtacc tgtcgctttt gcatcttcag tcgcgtcttc
    40561 acgacccggc gttttcagat ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac
    40621 aatggcataa ccgataccga cgatcgggaa cagccacagt ttgctgctgt taccagacag
    40681 aacgatgaag tcgatcagac cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat
    40741 acagattggg aatgccaggc ctgccagaat cgcgtggatg atgtacagga tcggcgcaac
    40801 gaacatgaag gagaactcga tcggctcggt gataccggtc aggaacgagg tcagcgccgc
    40861 ggagatcata ataccgccca ctttcgcgcg gttttctggt ttagcagagt gccagatagc
    40921 aattgcggca gctggcagac cgtacatttt gaacaggaag ccaccagaca gtttacccgc
    40981 agtcgggtca cccgccatat aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt
    41041 ggtgtattca ccaatttgca tctggaaagg tacgttccag atgtggtgca gaccaaacgg
    41101 taccaggcaa cgttcgatga aaccgtaaat gccaaacgca actaccgggt tctggtaagc
    41161 agcccactga gagaaggtct ggattgcaga accaatcggc ggccaaatga aggacagcac
    41221 aacgccagta aagatggcag ccaggccaga aatgatcggc acaaagcgtt taccggcaaa
    41281 gaagccaaga tactcaggca gcttaatacg gtagaaacgg ttaaacatat acgctgcaat
    41341 cgcaccggag ataatccctc cgagtacgcc agtatccgcc aggtgtttag aggcgatttc
    41401 ttcagcaggt aaatgcagta ccagtggcgc aaccacggcc atggttttaa ccatgatgcc
    41461 ataggcaaca actgcggcca gcgcggatac gccatcgtta ttggtaaagc cgagggcgac
    41521 accgatcgca aaaatcagtg gcatgtttgc aaagacggaa ccgcctgctt ctgccataac
    41581 atgcgataca acggcgggca gccagctgaa attcgcggaa ccgacgccca gcagaatacc
    41641 tgcgataggc agtacggata ctggcagcat cagcgattta ccgacctttt gcaggttagc
    41701 aaatgcattc ttaaacataa ttgagagtgc tcctgagtat gggtgctttt tttacgttct
    41761 cacgcgtggc aaggggggag tgcctcgccg tgtacagggc atctaagcgc cctttattta
    41821 ttacacagag taaaataatt cagtgccgat ttgtttgacg gctatcacgt ttcaacttca
    41881 gatcattgga gaagttgcac attttgacaa aacatatgtc acaatatttc aaacgtggtt
    41941 attcaccgcc cgattttcgg cggtgaactt tactcgtttt agccattaat tacgagcttt
    42001 aaaaaaactc attcaacgga tggattgaag gcgggaagcg tcgatgtgaa acagacgggc
    42061 gaagttatcg gtggttacct gcgccagttc ttcaacggca acacctttca acacagccat
    42121 gtattctgca acgtcacgaa ccatcgcagg ttgattctct tttcctcgat gcggtaccgg
    42181 cgcaaggtaa ggtgagtcag tttccaccag taaccgatcc agggggacat aacgcgcagc
    42241 atcgcgcagt tgctccgcat tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc
    42301 gagatccagt aatttacccg ccgtttctct gtcctctgta aaacagtgta gtacgccacc
    42361 gcaatccgtc actttttctt cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt
    42421 gtggacgata accggcttgt tcagttcacg accaatctgg atatgatgga tgaaggactc
    42481 ttgctgacgt actttagttt ccggcgtgta ataataatcc agcccggttt cacccagcgc
    42541 tacaacgccc tcttctgccg ccagacgacg taaatcttct acatcgtagg gatcattctg
    42601 gttgagtggg tgcacgccac aagaaaatac gacgttgtcg cgttcgccta ccagatcccg
    42661 catgtgtaaa taacccggta atgttgtggc gactgccaga caaaatttca catcgcgtgc
    42721 ggcggctttc gccagaacgt catccacgtc cttatgcaaa gattcataat ccagaccatc
    42781 gagatggcag tgtgagtcga ctaaaaacat gatgtctctc ttaaagatga ggaaccggta
    42841 gcacaacgcc cggttgcagg taatgctcaa tacgcagtaa aagatcggtg atgagaagct
    42901 cgcggttgat gcctgtgaca gacattaact gttcacgaat gtggcaaaca tcccccagta
    42961 tagcctgcag gcgcgaggga gaaagatggt ttgccagttc gacgaccagg cccggcacat
    43021 caacattggt cacctgcgca gcgccatgat ggcgttttag cgcatccatc aacaacgttg
    43081 ccagccagtg taaacgtgcc ggagcttgtt catgattaag ggccgctagc agcgaatacc
    43141 agtcgcccga ttgcacgcta tatgccagcg cctgacacaa tgtttcacga gcctgccagt
    43201 tatctccctg aaacaacgcc agtgccgccc caggcgaacc ggcgcttaag cgcaatgcgg
    43261 caagtagtgc atcctgtgac attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt
    43321 ccggcggcgg cgcaaggtaa tgtaaccgac aacgactacg taatgttgcc agtaaacgtt
    43381 ctggctcgcg ggtagcaagg aaaaaccacg tctctgctgg tggctcttca agcgttttca
    43441 gcaatgcgtt agccgcggcg tcggttagta aggcagcatc ggttacccag acaactttcg
    43501 caccacctaa gcgtgcgtgc tcattcagct tttcggtgac ctcacgtacc gcatcaatgc
    43561 ccagcgcatt tttccctttt tcgggagcca gggtgtagta atcgggatgt gtgccagcct
    43621 gcatcaactg acatccacga cagtgaccgc aacttttgtg gccctgcggt tgttggcaga
    43681 gtaaataacg gctcagggcg tagattaaag catcatcgcc catgcccggt aacgcctgaa
    43741 tgagtagcgc atggtgacct cttccggcct gatagctggc taccagtttt tcgaaatcag
    43801 gtcgtaacca tggataccat ctcatgcgtc caactccttc acccagttgg tcacggtagt
    43861 gcggattgca tccatcacga cctccagcgg ctgggtggca tcaatggtat gaatgctttt
    43921 atcttgtgct gccagttcca gatagcgggc gcgggtgcga ttaaagaaat cgaatgactc
    43981 ttgctcaata cgatccagct caccacgggc gcgagcacgt tttaagccga cctctggggt
    44041 gacatcaaga taaagcgtta agtcagggcg aaaatccccg agaacagcat cacgcagtgt
    44101 tgccagcata tgttggtcaa tacctcgtcc tccaccctga taggcttgag tagagagatc
    44161 gtggcgatcg ccaatcaccc aggtgccgtt agccagcgca ggtttgatga ccgtttctac
    44221 cagttgaacg cgcgcggcat aaaacatcag aacttcggct ttatcggtaa tgacttcatc
    44281 gcctaccgat ttgatatcca gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc
    44341 aggttcccga gtgaaaacca tgtcgcggat acccagttgc tcaagcgtct caaccaccac
    44401 gttacgcgcg gtagttttgc ctgcgccttc cagcccctca atgacgatat acttactgcg
    44461 catttttttc cttaagcact ttcagataat cctgcacaga cttgttatga ctggcaagat
    44521 tggtattaaa cgtgtgacca cctttaccat cggccacaaa atagagatac ggcgtttttg
    44581 ccggatgcgc agcagccttc agcgaatccg cccccggcgt agctattgca cctggcggca
    44641 aaccggtaat ggtataggtg ttatacgctg tcggcgtttc caggtctgca cgagaaagtt
    44701 tgccattata acgctctccc atcccgtaaa tcacggtcgg gtcggtctgc aggcgcatac
    44761 caatgcgtaa ccggttgata aataccgagg caacctgatc gcgttcactg gcaacggcgg
    44821 tttctttttc gataattgat gccatcgtca ccaactggtt tttatcttta taaggcagac
    44881 cgtccgcacg cccttcccag gcgctatcga ccgctttcac cattttcttg tgcgctcgct
    44941 tgagtaacgc gacatcggtg gtattggcgg tatacatcca ggtgtctggc cagaaccaac
    45001 cttcaatcca ctccgggttt tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt
    45061 cgctcagcgt gtgcttgata tacggagcct cacgcaattg cttgaggtag tcgctcagac
    45121 gcatcccttc taccagtcgc agcgggaact gtgcttcttt accgctttcc aacaatttca
    45181 gcatctcgcg cacggtcatc tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa
    45241 gatccggttc gatacgcagc aaccattgaa aaacccgtgg gcgattgatg atcttatcgg
    45301 cataaagctg ttcacctagc gccaaacgtc cggtccctgg cttcagggta aatatcgtct
    45361 cttctttgat aagcaatttg ctgtcggcaa gatggcgaac cttccagacg cccacaccag
    45421 cggcgatacc cagtaccacc agcaataaca aaattatcaa taacactttt ttcatgacta
    45481 attcgggcgc tcacaaagtg gggctaaata ttcatataac gttgctgacg aaaaggaggc
    45541 atcgccacag gcacgcacgg gcatcactgg cattaacgca ttacaaataa ccatctcatc
    45601 tgcctgcaac gcctcttcca gagaggcttg cacttcgaca agctgataag aggattgtgc
    45661 cagcaaacgg atacagaatt gtcgcataat gccgttaaca cctgcctgat ccagtcgcgg
    45721 cgtataaact acgttgccct tccgccagaa caaattagcc gcacagcatt ccgtaaccca
    45781 cccttcgctg tcaaggacca gcgcctcatc agcgtttgtc tgctcaagat gagagcggat
    45841 caatacttgc tcaagccgat taagatgttt aatacctgca aggtgaggat tgcgccccag
    45901 ccgcaccggg cttagcgcca acatcatccc ttcgttacgc aaacggtcgt aatgtgcagg
    45961 ataagccgta atggagagaa tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg
    46021 cccgccacta ccgcgactga tcacgacttt cagcacacca ttctgctgtt ctgctgccag
    46081 cgttttcatc tcttgttcaa gctgaggcca gaagtcacag gaaatcatca accgctgaca
    46141 agcatcctgt agtcgctgga tatgcgccga taacaaactg actttaccgt cgataactct
    46201 ggcggtggta aaacaaccat caccaaactg cgttgcccga tcgcttactg ccagcgattc
    46261 ctgcttatga ccgttaatta agaacatacc agctccttat catagaattg ttagtgtggc
    46321 agcatgttca ctacggaaca agtcggaata aaagctaaga aaaaaggccc gcaagcggac
    46381 cttttataag ggtgggaaat gacaacttag atctttttaa agatcaaaga accattagtg
    46441 ccaccgaagc cgaaggagtt acacagcgtg tattccattc cgctgacctg acgtgcttcg
    46501 tgcggtacga aatccagatc gcaaccttca tccgggttat ccaggttgat ggttggcgga
    46561 acagcctgat cgcgcagcgc caggatggag tagatagatt ctactgcacc cgccgcacct
    46621 aacaggtgac cggtcataga tttcgtggag cttaccaaca cacggcttgc agcttcaccg
    46681 aagatggttt tcaccgcctg cgcttcagct ttatcgccag ccggagtaga agtaccgtgt
    46741 gcgttaacgt agccaatctg actcgcttca atgcctgcat cacgcaaagc atttgccatt
    46801 gccagagctg cgcctgcgcc attttctggc ggtgacgtca tatgataagc atcgctgctc
    46861 ataccaaagc cgacgagttc agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac
    46921 tcttcaagta ccagcatacc ggcaccatcg cccagtacga aaccatcacg ctctttatcc
    46981 cacgggcggc tcgccgcttg cgggttatca ttgcgggtag ataatgcacg tgccgcgcca
    47041 aaaccaccaa cgcccagcgg cgtactggct ttctctgcgc cacctgcaac catcacgtca
    47101 gcatcgccat acgcgataat acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag
    47161 gcggtcgcga tagagatgct tgggccacgc aggccataca tgatagtcag atgacctgcc
    47221 accatgttca caatcgttga cggaacgaag aatgggctga tcttacgtgg accaccgttc
    47281 atcagagatg tgtggttttc ttcgatcagt ccgaggccgc caatcccgga gccaattgcg
    47341 gcaccaatgc gggttgcgtt ctcttccgtt atttcaaggc cagaatcctg catggcctga
    47401 acgccagcga caattccata ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag
    47461 ataatgtcct cacagttaaa atcctttact aagccagcaa atttcgttgc ataggcgcta
    47521 gtatcgaaat ggtcgattag gctgatgcca ctctgaccgg caagcagagc tttccaggta
    47581 gactctacgg tattgccgac aggagacaac atgcccagtc cggtcacaac tacacgacgc
    47641 ttagacacgt ttgtcctcca gggagggaaa aaatgattct agtgggacaa aaagataaaa
    47701 ctcaggcggt cgaacgaccg cctggagatg ttcacttacg cctggtggcc gttgatgtaa
    47761 tcaatggcag cctgaacggt ggtgattttc tcagcttctt cgtccggaat ctcagtatca
    47821 aactcttctt ccagagccat taccagctca acggtgtcaa gagaatccgc gcccaggtct
    47881 tcaacgaaag aagcattgtt ggtaacttct tcctgcttaa cgcccagctg ttcgccgata
    47941 attttcttaa cgcgttcttc gatagtgctc atactcttaa atttcctatc aaaactcgct
    48001 ttcgcgatgg ttttcgtagt gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg
    48061 tcttaccacg attttacgtt attttgaggc cttacccccc aataacgcaa atcattttgc
    48121 actaattatt actcataacc acgcagactg cgcgcaacat gagcattttg tgcaaatcgc
    48181 ggtcagacca tgtacatccc gccgttcaca tgcaaagttt cacccgtgat gtaagctgct
    48241 tcgtcggatg ccaggaatgc aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc
    48301 gcaggaacct gcgccaggat acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc
    48361 gtttcaataa agcccggagc aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc
    48421 gccagtgatt tactgaagcc gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg
    48481 ccatttccca tggtaccaac cacagaaccg atagtgataa tacgaccatg acgctttttc
    48541 atcatagcgc gcattaccgc ttttgacaga cggaaaacag atgaaaggtt ggtttcgata
    48601 atatcgttcc actcttcatc tttcattcgc attaacaggt tatcacgagt gataccggca
    48661 ttattgacca ggatatccac ttcaccaaat tctgcgcgaa ttttttccag aacagattcg
    48721 atagatgccg ggtcggtcac attcaacatc agacctttgc cgttagcacc taaataatca
    48781 gtgatcgcct gagcgccatt ttcactggtc gcagtgccaa taactttcgc gccacaggct
    48841 gcgagcgttt cagcaattgc gcggccaatt ccgcggcttg caccggttac cagtgcgatt
    48901 tttccttcaa aattcatgat tttcctcttt taaagctcga gcgccgctgc catcgctgaa
    48961 ggttcgttca gcgccgaggc ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc
    49021 actttgcccg ggccgacttc atagagatgt tctacgcctt gcgctgcaat gtactcaaca
    49081 gacttcgtcc actgaaccgg gttatacaac tgacgtacca gtgcgtcacg aatggcatca
    49141 ccattggttt cgcatttcac atcaacgtta ttcacaacag gaactgttgg tgcgttaaag
    49201 gtgattttcg ctaattctac tgccagtttg tcagctgctg gtttcatcag cgcacagtga
    49261 gatggtacgc tcactggtaa cggcagagcg cgttttgcgc ccgcagcttt acaggcagcg
    49321 ccagcacgct caaccgcttc tttatgaccg gcaataacca cctgtcccgg agagttaaag
    49381 tttaccggag aaacgacctg accttctgca gcttcttcac acgcttttcc aatagacgca
    49441 tcatccagac cgatgattgc cgccatagcg cccgttcctt ccggtacggc ttcttgcatg
    49501 aacttgccgc gcatctcaac cagacgcacc gcatcagcga aatcaatcac accagcgcaa
    49561 accagcgcgg agtattcccc caggctgtga ccggccatca ttgccggtgc tttaccgccc
    49621 tgctgctgcc atacgcgata cagcgcaaca gatgcagtca acagcgctgg ctgggtttgc
    49681 caggttttat tcagttcttc agctggcccc tgctgggtca gcgcccacag gtcgtagccc
    49741 agcgccgcag aagcttcagc aaacgtttct tcgacaattg gatagctcgc cgccatatca
    49801 gccagcattc caacggtttg agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg
    49861 ttttaatcct tatcctagaa acgaaccagc gcggagcccc aggtgaatcc accgccaaag
    49921 gcttcaagca gaaccaactg tcccggctta atgcgcccgt cacgtacagc ttcatccagc
    49981 gcgcacggga cagaggccgc agaggtatta ccgtggcgat ccagcgtcac cacaacattg
    50041 tccatcgaca tgccgagttt tttcgccgtt gcactgataa tacgcaggtt agcctgatgc
    50101 ggaaccagcc agtccagttg agaacggtca agattattcg ccgctagcgt ctcatcaacg
    50161 atgtgcgcca gttccgttac cgcaaccttg aagacttcgt tacccgccat tgtcagatga
    50221 attgaattct ctggattcac acggtcagca ttaggcagcg tcagcaattc accatagcta
    50281 ccgtcggcat gcagatgggt ggagatgatt cccggctctt cagaggcagc cagcaccgca
    50341 gcgcccgcgc catcgccaaa aataataata gtcccacggt ctgttggatc gcaggtgcgc
    50401 gccagtacat cggaaccgac gaccagagca tacttcaccg ccccagattt cacgtattga
    50461 tcggctacgc ttaatgcata ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg
    50521 caacctttaa tgcccagcat gctttgaatc tgacaagctg cgctcggaaa agcgtgcgta
    50581 gcagaagtcg ttgccacaac gatcaggcca atctggtctt tctcaatgcc cgccatctca
    50641 attgcacgtg tcgccgcttc aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg
    50701 tggcgttcgc ggataccggt acgagtgaca atccactcgt cagaggtgtc caccattttt
    50761 tccaaatcgg cgtttgtccg cacttgttcg ggcagatagc tgccagtacc aataatcttc
    50821 gtatacatgt acgctcagtc acttttcggt tatataccgt cacttgcaaa ctgcgagttc
    50881 gctggcagcg tcctgctacc gcagagttcc gcttttgcca ccgtccagca gctcaaaacc
    50941 agctgggtat acagattcca ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc
    51001 ctgcactgcc tgttcaatcg cgaccgcaaa agctcgctga ttggctgcac catgactttt
    51061 tatcaccgtg ccgcgcaatc ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg
    51121 actgaatcgc ctcgtcaggc tcttttgtag ccaacgcttt aataacagta gccaccacga
    51181 ccgttttttc ccttcaccct gagatttcag cagagaaagg aacatcctga caacaccttc
    51241 catcgtcttt aatgtgacat ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc
    51301 agttaacaac tcattggctt caagatagcc gatataattg atagaaggga ttgttttaag
    51361 caccgctgag gcatcccgaa tactgtcgag accctttact tcttcttcac caatattgag
    51421 caacgccacg cgaggattag gaatttccac cacctcttca gccagaactg agcccataat
    51481 ggcaaattgc accaacattg tactgtcaca atcgacgttg gcccctaaat cgaggaccac
    51541 cgttttgccc ttttgctgat gcggtaatac cgtcaccagc gccggacgct caatcccctc
    51601 caggggcttg agtaataatt ttgccagccc cattagcgcc ccggtattac cggcactgac
    51661 acaggcttgc gctcgacctt ctttcaccag ctccagggcc acgcgcattg aactcccacg
    51721 actggcgcgg atagcttgcg aaggccgggc atcactggcg ataactgact gcgcaggaat
    51781 aatctgcaga cgcgaacgtt gttcaaagtc agctttagca agtaatggcg tgatggcgtc
    51841 gggattgccg actaaaagaa gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc
    51901 aggcactgtc acggaagggc caaaatcccc tcccatgaca tctagcgcca gggttagacg
    51961 tgtcaaggta tcgtcgccgc ccggtttggt ctttccccag ttgcctgggg aaaatcctca
    52021 ctaagcttca tcacgcagac gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa
    52081 ccgtcggcag tgatgtggtg acgcaggtgt ttttcaccag aagttttgtc tacagacagg
    52141 ctggtgactg cggtcagcgc gtcatgggaa cgacgcatgc cacgtttgga acgggttggt
    52201 ttattctgtt gtacggccat ggaccttact cctcaattac ggtttatccc cgatccaacg
    52261 gggagcacca attacttacg ctttaagctg gctaatacgg caaatgggtt tggcttttgc
    52321 gcttcttcag gcagttcacc aaagaccatg tccgcttcgg acacttcaca gtgttcagaa
    52381 tcatgcaccg gaactaccgg caaggcgagg atgatttcat cttcaaccat tgcaagcaga
    52441 tcgatttcac cgaattcgtt aacctcaatc ggttcatacg cttccggcag tgcttcagcc
    52501 tgttcgtctg aacgcacagg actaaaacaa tacgttgtgt agacctgatg agtaaacggc
    52561 ttcccgcaac gctgacactc gagcgttacc gtcaccttcg catcgccgtt taacactgcg
    52621 agacgttggt tatcgatagc gaacgacatg gagcattcca catcactgtc cacactgact
    52681 acggattcgg cgacgcgctc aacctgatca ggagtataga taccctggta atcaaggcgt
    52741 ttttgagccg tacgaaccgg atcgagagtc aggggtaatt ttaccttttg catagggcgc
    52801 gcatattaac tttgtaacgt catagagtca aagaaaaagg cagcctgtgg ttgccttttg
    52861 ccaataattc gcacacattg cgggttactg ctttattttc gttcagtgct gataacgaac
    52921 atcaacctgc aaaacgaagc gcatagttta aaatggctat catcaatacg ctatatctga
    52981 tggaaaaaaa tatgcctaaa cttattttag cctccacctc gccctggcgt cgcgctcttc
    53041 tggaaaaact gcaaatctct tttgaatgtg cagcacccga ggtcgacgaa actccacgca
    53101 gcgacgaatc accgcgacag ttggtgcttc gactggcaca agaaaaagcg caatctctgg
    53161 cgtcacgtta tccggatcat ttaattattg gttcagacca ggtatgtgtt cttgatggcg
    53221 aaatcaccgg taaaccgtta acggaagaaa atgcccgtct gcaattacgc aaagccagcg
    53281 gcaatatcgt caccttctat actggactgg cgttgtttaa ttcggcgaat gggcatctac
    53341 aaacagaagt ggagcctttt gacgtccatt tccgtcacct gagcgaggcg gagattgata
    53401 attacgtgcg taaagagcat cccctgcact gcgtgggtag ctttaagagt gaaggatttg
    53461 gcattacgct gtttgagcgt ttagagggac gtgatcctaa cacgctggtt ggtttaccgc
    53521 taatcgcgct gtgtcagatg ttacgtcggg aagggaaaaa cccgctgatg ggatagtttt
    53581 ttgcgccgga tgcggttact tcgcatccgg caatcggtgc tgtcggcctg atgcggcgta
    53641 aacgccttat caggccaacc gcccattatc aggtttatat gcttgtatta gcgcgcgtta
    53701 cgcagctttt gcaaacaacg cttcaaacct tcatccatcg gcgcttcaat acgcatcacc
    53761 tcaccggtcc ccggatgagt aaacttcaac gctgcggcgt gcaggaacag gcgatttaat
    53821 cccgtgcctg cttcagtgag ctgtctgtca aattcaccgt caccgtagcg atcgtcaaag
    53881 gcaatcggat gacccgcata ttgtgtatgc acacggatct gatgagtacg ccctgttacc
    53941 gggctacaac gcaccagggt ggcaaatgca tagcgctctt ccactttaaa gcgtgtttcc
    54001 gacggtttgc cttcctgact cacacgcacg atacgttcgc cgctttgcag aatatttttc
    54061 agtaacggcg cttgaacgct cttcacatgc gactgccact gaccgcgcac cagcgccagg
    54121 taatcttttt gcatcccttt ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt
    54181 tttttcgcta ccagcaacac acctgaggtg tcccggtcaa gacgatgaac cagttcaagg
    54241 aaccgcgctt ccgggcgcaa cgcccgcaaa ccttcaataa cgccgaagct taaaccactg
    54301 ccgccatgta ccgccgtacc ggaaggttta ttcagcacca ggatatgatc atcttcatat
    54361 aagatgacgt ccgccagcgc cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc
    54421 cgctcagcaa cgcgaaccgg tgggatacgc acctcatcac ccgcttcgag tttatattca
    54481 ggcttaatac gttttttgtt cacccgcact tcgcctttac gcaaaatacg gtaaatcata
    54541 ctttttggta cgcctttcaa ttgggtacgc aaaaagttgt cgatacgttg ccccgcttcg
    54601 tcagcggtga tagcaacaat ttttacggat ggagtctctg ttttcatggt gggcgattct
    54661 aattagccaa caggattcgc gccactcatt tttctatgct tatatttagt tttacacctt
    54721 attacttcac taggtgatca ctttattgat ggttattaaa ccaatcacca gcaagaagtg
    54781 aaaaaactgt gagtaagcgg gtgataaatg gtaaaagtca tcttgctata acaaggcttg
    54841 cagtggaata atgaggccgt ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa
    54901 taacccattt tgcccgaccg atcatccacg cagcaatggc gtaagacgta ttgatctttc
    54961 aggcagttag cgggctgcgg gttgcagtcc ttaccggtag atggaaatat ttctggagag
    55021 taatacccag tctgtttctc tgataattgc gctgtttttc cgcatgaaaa acgggcaacc
    55081 gacactctgc gcctctttga gctgacgata accgtgaggt tggcgacgcg actagacacg
    55141 aggccatcgg ttcacacccg gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa
    55201 gaataatgag taagttacga tgaaaagaat gttaatcaac gcaactcagc aggaagagtt
    55261 gcgcgttgcc cttgtagatg ggcagcgtct gtatgacctg gatatcgaaa gtccagggca
    55321 cgagcagaaa aaggcaaaca tctacaaagg taaaatcacc cgcattgaac cgagtctgga
    55381 agctgctttt gttgattacg gcgctgaacg tcacggtttc ctcccactaa aagaaattgc
    55441 ccgcgaatat ttccctgcta actacagtgc tcatggtcgt cccaacatta aagatgtgtt
    55501 gcgtgaaggt caggaagtca ttgttcagat cgataaagaa gagcgcggca acaaaggcgc
    55561 ggcattaacc acctttatca gtctggcggg tagctatctg gttctgatgc cgaacaaccc
    55621 gcgcgcgggc ggtatttctc gtcgtatcga aggcgacgac cgtaccgaat taaaagaagc
    55681 actggcaagc cttgaactgc cggaaggcat ggggcttatc gtgcgcaccg ctggtgtcgg
    55741 caaatctgct gaggcgctgc aatgggattt aagcttccgt ctgaaacact gggaagccat
    55801 caaaaaagcc gctgaaagcc gcccggctcc gttcctgatt catcaggaga gcaacgtaat
    55861 cgttcgcgca ttccgcgatt acttacgcca ggacatcggt gaaatcctta tcgataaccc
    55921 gaaagtgctc gaactggcac gtcagcatat cgctgcatta ggccgcccgg atttcagcag
    55981 caaaatcaaa ctgtacaccg gcgagatccc gctgttcagc cactaccaga tcgagtcgca
    56041 gatcgagtcc gccttccagc gtgaagttcg tctgccatcc ggtggttcga ttgttatcga
    56101 cagcaccgaa gcgttaacgg ccatcgacat caactccgca cgcgcgaccc gcggcggcga
    56161 tatcgaagaa accgcgttta acactaacct cgaagctgcc gatgagattg ctcgtcagct
    56221 gcgcctgcgt gacctcggcg gcctgattgt tatcgacttc atcgacatga cgcctgtacg
    56281 ccaccagcgt gcggtagaaa atcgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat
    56341 tcaaatcagc catatttctc gctttggcct gctggaaatg tcccgtcagc gtctgagccc
    56401 atcactgggt gaatccagcc atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg
    56461 tgacaacgaa tcgttgtcgc tctctattct gcgtctgatc gaagaagaag cgctgaaaga
    56521 gaacactcag gaagttcacg ccattgttcc tgtgccaatc gcgtcttacc tgctgaatga
    56581 aaaacgttct gcagtgaatg cgattgaaac tcgtcaggac ggcgtgcgct gtgtgattgt
    56641 gccaaacgat cagatggaaa ccccgcacta ccatgtgctg cgcgtgcgta aaggggaaga
    56701 aactccaacc ttaagctaca tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc
    56761 tgaagaagag ttcgctgaac gtaagcgtcc ggaacaacct gcgctggcaa cctttgccat
    56821 gccggatgtg ccgccagctc caaccccagc tgaacctgcc gcgcctgttg tagccccagc
    56881 acctaaagct gcaacggcaa caccagcatc tcctgcacaa ccagggttgt tgagccgctt
    56941 cttcggcgca ctgaaagcgc tgttcagcgg tggtgaagaa accaaaccgg ccgagcaacc
    57001 agcaccgaaa gcagaagcga aaccggaacg tcaacaggat cgtcgcaagc ctcgtcagaa
    57061 caaccgccgt gaccgtaatg agcgccgcga tacccgtagt gaacgtacag aaggcagcga
    57121 taatcgcgaa gaaaaccgtc gtaatcgtcg ccaggcacag cagcagactg tcgagacgcg
    57181 tgagagccgt cagcaggctg aggtaacgga aaaagcgcgt accaccgacg agcagcaagc
    57241 gccgcgtcgt gaacgtagcc gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc
    57301 gaaggcgctg aatgttgaag agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg
    57361 tccggttcag ccgcgtcgta aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag
    57421 cgtagccgaa gaagcggtag tcgcaccggt ggttgaagaa actgtcgctg ccgaaccaat
    57481 tgttcaggaa gcgccagctc cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc
    57541 gcaagctgca ccagaacagc aagaagagaa caatgccgat aaccgtgaca acggtggcat
    57601 gccgcgtcgt tctcgccgct cgcctcgtca cctgcgcgta agtggtcagc gtcgtcgtcg
    57661 ctatcgtgac gagcgttatc caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc
    57721 tccggaactg gcctctggca aagtctggat ccgctatcca attgtacgtc cgcaagatgt
    57781 acaggttgaa gagcagcacg aacaggaaga agtacaagtg cagccgatgg tgactgaggt
    57841 ccctgtcgcc gccgctgtca aacctgttgt tagcgcgcca gttgttgaag aagtggctga
    57901 agtcgtagaa gtccccgttc aggttgccga accgcaacct gaagtggttg aaacgacgca
    57961 tcctgaagtg attgctgccg cggtaactga acagccgcag gtgattaccg agtctgatgt
    58021 tgccgtagcc caggaagttg cagaacacgc agaaccggtg gttgaaccgc aggaagatac
    58081 ggcagatatt gaagaagttg ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt
    58141 tgctcaacct gccgcgccag tcgtcgctga agtcgcagca gaagttgaaa cggtagctgc
    58201 ggttgaacct gaggtcaccg ttgagcataa ccacgctacc gcgccgatga cgcgcgctcc
    58261 ggcaccggaa tatgttccgg aagcaccgcg tcacagtgac tggcagcgcc ctactttcgc
    58321 cttcgaaggt aaaggtgccg caggtggtca tacggcaacg catcatgcat ctgccgctcc
    58381 tgcgcgtccg caacctgttg agtaataatt agctcaagta atcaagccct ggtaactacc
    58441 agggcttttt tatttcatct ttgagtcaac atattaaaat tcatcaaatg gcatccttgc
    58501 taaccaacaa tacaaaatag gcaaaaaaat gcccgccccg gcgatcccgg agcaggcaga
    58561 caaaacatac ccagtttcat gatatgtttt aaagcgaaat tatttgttga gctggaagag
    58621 cgacaatccc tgcatatcgg taaatgcttt atacgacgcc tgcaatgccg tttgctgcat
    58681 gatgtaagat gaaatagttg cattccagtc cacatcaacc agatcgctca tctgctgcgt
    58741 ttgccctaaa gcgcgatcgc tacctaagga atccagcgac tccagttcgt tcagctgcgt
    58801 gcctaattcc gcgcgaacag tcagcacatt gttcagcgag tttttcagtc cgcggttggt
    58861 tttatccagt gccgcagcgg cggtttcttt atccgcttcg ctatccgcga ccggcgtttt
    58921 cagggctgcg atggcgctat ccagcatggc aaaaagattg gtttcagaag cgctaccgtc
    58981 tggttccgct accgcgttgc tggtaatact gtcgaaaatt ttgtcacccg tgtgccctat
    59041 caccatcgaa cgcgaagcat cgacctgttg tttaatactt tctgctccgc cttcgtattt
    59101 gccatccgcc tcgctaaacg gtgcggattc tgttttataa ccggcaaaaa tgtagcgccc
    59161 gttaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct gaatatccgt
    59221 agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga caattttttc
    59281 ctgagcattc tggatagcgg tggtgacctg gctaagtaca ctctcttcca gtgacacttt
    59341 ttgagtggcg aaagtacgcg ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag
    59401 aacaacagct tgtgatgcag caatgggatc atctgaaggg ttaacgactc gcttacccgt
    59461 cgacatctgt tcaccgtact tcatccattc tgcctgagaa ttggtgatgc cgcgcatgtt
    59521 ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg aatgttaatc
    59581 agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag gtaatactgc
    59641 tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat cgactgctgc
    59701 tgattggaaa gctgcgtcac cacattacct tgcgtggcgc tactggtttt caacgtcgcg
    59761 gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga tttcgcaccg
    59821 cccaccgttt tactgttgct ttgcagatcc agcagggcct gaccgttgcg gttgtcgcta
    59881 tcacccgcat cttctgcgct cgccatggcg attttcgctt cgtcagtgat taatacatcc
    59941 atgttgacga tggcgtcact taccggtttc aacgtgaagc tgtcgttaac ggcaggcgtt
    60001 cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc cggcgtcacc
    60061 gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc gaacgagatt
    60121 ttgtaatctg tcgccagtac cgcggaggca tcagttaccg ttgcaccgat cgcaacgtca
    60181 ccgttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagcaaagaa atcttcaccg
    60241 gcatcgccgt tagcatcaaa tccggctttg tgttgggtgt tgaaagcccc ggcaaatgcc
    60301 agcgccagtt gtccgagcgt attacgcgtc tggtccagat cctgagaacg gaatgtcaga
    60361 atgccgccca gcgacccggt attcagcaat ttctccggga tctcaatatt gcctgccgta
    60421 ccatcaacgt aagcgacagt cgtacgagaa gggtcagcgc tggaaggaac tgccgccagc
    60481 tgccgcgccg tacttccctg aaccagtgag tagccattgg ccatcgtgat gttataagtg
    60541 ccaccatcct gaacgctaac ttctacgcca acaatctggt ttaattcact caccagttga
    60601 tcgcgttgat ccagcagatt gttaggtgac gcccctgccc ccacgcctgt cagacgcgag
    60661 atttgatcgt tcaggctggc gatttgttta gcgtaattgt tgatctgatc aacgctggca
    60721 cctatcgcga tattgacctg tttgtcctgg tcgcgcagat attgatcggt ggttttaaac
    60781 tgattcacca acccttctga tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg
    60841 ttactcacca gcgtttgcag gctggtgaag aaatcctgca tctgtgttgc cagcgaagag
    60901 gtactggtgg agagcatatt gtcgattttc gacatctgct catagcgggc agtcaggccg
    60961 ctactttgcg tctgcgcagc acgtaactgg ttggtaataa acgcatcata ctcacgctgc
    61021 acaccagaaa cgtagacgcc attgccaacc cagccgccag cgcccaacgt gctattggcc
    61081 tgcgccataa tagtggtttg gcgggtatat ccggcaacgt tatagctgga gatattatta
    61141 cttgccgtat ttaacgccgc ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag
    61201 ctggacatgg aggttcctta taagccttca attacagagt attatcggca gcgacccgcc
    61261 ggacttgagt tattcagaac agattatcaa tgttcatgct gtaggttttg ctcaccttgt
    61321 cgcttatcga tttcatctgc tgaatcatgc tggtgagttt gcgggcatag tgaggatcgg
    61381 tggcatagcc cgcgtcctgt agggcctgcg ccccctgctc cgcactcgcg gcggtcgtca
    61441 cggcggcgta gcgcgggtta cgcgttaaca gcccaacgta atccgacaat gcttccagat
    61501 acgagctgta gacccgaaac ttcgctttta ctttcttcgc ttcgccattt tcatattcag
    61561 tcgtggtgat ttcagtgact ggccctttcc agttgccaga ggctttgacg ccaaacaggt
    61621 tatagctcgg ctcgccgttt tcacggcgga tttgccgttg tccccagcca gattcaagcg
    61681 ccgcctgagc gaggatcaaa tgatgtggca caccgctttg ctggcttgcc agttgggcgg
    61741 gcaacgagag ttgcgcgagg aatgctttac tgtcacccgg cagcgaatca tcgtagttac
    61801 gtggtacggc cttttgcacc agctgcgaaa gcgtctgatt ttgataacgc accacggttt
    61861 cgagcgggaa tttcatcggt gctgctggcg tggactcctc tggcaatggt tgttctggcg
    61921 tcatctgttt aaccatcatc tctgccagcc ccagaccttt gcccgccgtc atctgttggg
    61981 caatctgctg gtcatacata ctggtataca ggcgagtgcg ctcgctgctg aacaggccat
    62041 cttttggtaa agcgtcgcgc atgcttttca acatcatctg cacgaacatc ccttccacct
    62101 ggcgggccac cggacggata tttgccgccg gatcttcgcc cgctttcgcc ttaagttcgt
    62161 tgagcgattg tgcatcccag gccgcacttg ccagtagttt gctgtcgctg atcatcagat
    62221 gatttccagt tttgcccgca gacatcccgc actttgcatt gattgcagta tggacatcag
    62281 atccatcggc gtagcgccca gcgcattgag cgcacgcacc acgttattga ggctggcgct
    62341 ggaacgtacg ctttgcagcg aaccgccgct ctggcgtaaa tcgatctgcg tttgtggcgt
    62401 taccacggtc tgcccgccac caaacggtgt atctggctgg ctgacattgg cctgacgatt
    62461 aactgttact gagagattcc cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt
    62521 catcaccacc gaaccagtgc gcgagttaat cactacttta gcgtcctgcg gggtgacatt
    62581 aacctgcata ttctggatat cggcaaggaa acggacctgg gaactgttgc cacttggtac
    62641 gcgcacctga atagtccgcg catctaacgc ggtggcgctg ccatatccac gcacgcggtt
    62701 gatggtgtca gcgatttgct gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag
    62761 ggtattcccg acgccaaact ggctgggcaa ttcacgttca ataaccgcac cattggtgat
    62821 ccgtccaccg ttcagttggt tcacctgaac actgctaccg ccagcggagg ctccagcgcc
    62881 accaaccaga atattgccct gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg
    62941 tgtcatcaac aacgtaccgc cacgcaagct tttggcatta cccatggaag aaaccaccac
    63001 atcgatggtt tgcccctggc gtccaaacgg cggaagtgac gctgtcacca ttaccgcagc
    63061 gacgtttttt agctgcatat tggtgcccgt cggaacggta attcccagct gtgagagcat
    63121 gttattaagc gtttgtgtgg taaacggcgt ctgggttgtc tggtcaccgg ttccatccag
    63181 ccccaccacc aggccatagc caatcagtga gttttgcctt accccctgaa cactggtgag
    63241 atcgcgaata cgctcagcct gagccgccgt gataaccagt agaagaatta atgcagagag
    63301 aaatttaatc accacagcct cacttacatt ggcgacaggt taaggaagaa acgctgcaac
    63361 cagcccatat tttgcgcttc gttaatgtag ccattgccta cgtattcaat gcgcgcatcc
    63421 gccacctgag tagatggtac ggtattgctg ccgctgatag tgcgtggatt aaccacgcca
    63481 gagaagcgaa taaattcggt accctgatta atggcgatct gtttttcacc caccacatgc
    63541 aggttgccgt tgaccagtac ctggtcaacc gtcaccgtca acgtaccgct aaaggtattg
    63601 ctggcattgg ccccgccttt tccgttgaac gtgttaccac cggaggcttc gacatcggca
    63661 cgagcgttac caaacaaccc ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt
    63721 ttaccgtcac ggctggcatt cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac
    63781 acgatagtca gcgtatcgcc aatattgcgt ggtcgacgat cttcaaacag cggttgatag
    63841 ccatagttaa tcggctgagc agactggaaa atagacccgt tggcgacggg cgtcggaccg
    63901 ggaaccggtt gtgcactggt cgccccttgt accagcggcg tggagggtat ccaggcgcag
    63961 ccggttagtg aaagcaccaa caagctggaa atggcataag tatgcgcagc gtttttttgc
    64021 atggctatca tcttcaaaaa tcagtaaacc ggtgaacctg ccaccggtta agccttagag
    64081 ttgcgtcagt ttttgcagca tctgatcggt ggtggacacc gctttactgt tgatttcgta
    64141 agcgcgttgc acctgaatca tattgaccag ttcttccgcc acgttgacgt tagacgtttc
    64201 aacataccct tgatacagca gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtgc
    64261 accggaggat tgcgtttcgg tgtagaggtt ttcgccaatg ctctccagtc cggtgtcatt
    64321 cataaaggtg gtgagattga gctgcccaac ctgaaccgga gctgcctggc cttgttgggt
    64381 tacgctgacc acgccatcac gaccgatggt gatacttaac gcattcgccg gaatggtgat
    64441 cgctggctgc acctgaaaac caccggccgt caccagctgc ccgttctgat ccacctggaa
    64501 agagccgtca cgggtatagg ctgatgagcc atctggcaac atcacctgga aaaagccctg
    64561 ccctttaatc gcgacgtctt tactgttatt ggtttgcgac aggttgccct ggctgtgtaa
    64621 acgttccgtg gcgaccgggc gtacccccgt accgatttgt aatccggatg gtaaggtggt
    64681 ttgttcggaa gactgtgccc ccggctggcg aatggtttga taaagcagat cttcaaacac
    64741 cgcgcgctga cgcttaaaac cgttggtact aacgtttgcc aggttgttgg caatgacgtc
    64801 catattggtt tgctgggcgt caaggcccgt tttggcgatc cataatgaac tgatcataaa
    64861 atgtcctgta gtggattagt gacaaagtgc atatgtcttc ttgccggatg cggcgtaaac
    64921 gccttatccg gcctacaaga gctcgcaaaa tcgataaatt gcgattgccc ccatagaccc
    64981 gataaacttg cgcatagggc aacttatact tgtcatgtat cctttcaatt aactcatcga
    65041 cagcagttgg ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat
    65101 ttcaaaacgc cgtgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact
    65161 gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta ccggcccacg
    65221 cctagcctgg ctttctgcgc ttaaacgaaa aatgccatcg tcaccgcgct ggacttcgct
    65281 gcccgtggct ttcaccagct tcagacgccc cactggcgca accgtatttg ccggatcgcc
    65341 cggattcagc gccgagattg tgccatcggc agcaatagtg atctcctccc cttccggcac
    65401 agcaattggc cccgcctcgc ctaccaccgg atgtccctga attgtcagtt gcccggtagg
    65461 atcaacctga atgctgccat tacgcgtata cccttcactg ccgtcagcgg tctgcacggc
    65521 cagccaacca tcctgctgca acgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc
    65581 gggcgtcatg tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag
    65641 cccttccacc ggcactgcgc gcaaggcgtt taactgcgcg cgaaaaccgg gcgttgaggc
    65701 attggccaga ttattggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat
    65761 cgcggtataa attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca
    65821 gcgtgttgag gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct
    65881 gggcaacgat catattgacc agttctttac tgagatcgac gttggacgct tccagcgcac
    65941 cgttggtcag tgtgccaaag ttgcccgtcc cggctgtccc taacagcgcc acgccagaag
    66001 attgcgtagc ggaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt
    66061 tcgccagtac aatctgcccc agcagttggg tttgttcgtt ggaatagttg ccgacaaccg
    66121 taccgtcatc attgatttga taactcacca gatcgcccgg tttgtagccg ttctgggtgg
    66181 ttgccacaat gttgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa
    66241 acgtggcggg atctgcgccg ttaattgcgc cagtggaaat attcgctgtt ggatttgagg
    66301 tcagaacgcc attggcatta aacaccagcg tcgttgcagg gttagctgtg cctgttggat
    66361 cactgctatc cagggtgtag acctgccagt tattatcccc ggtcttcaca aagtagacgc
    66421 tcatatcatg agcattaccc tgactatcga aaacagtcac cgaacctttt ttgttatagc
    66481 tatccgcatt gctggcatca aatgcgttaa cagagggaag cgaatcactg gaattcaggt
    66541 tgatctgcat cgacgccgtg gtggtagttt tcgctgccat cagggtattc ggaatcgaaa
    66601 tattggtcgg attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaccccg
    66661 tcagctgtaa accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt
    66721 tacggctgta gaacaccgaa ccattgctgt ctaccagacg gaaaaaaccg ttctggctaa
    66781 tagcaacgtc cagaccacgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga
    66841 taccagcaac ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg
    66901 ccgtgcctga tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga
    66961 ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct
    67021 gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag ggtagtggtg ccgtaagtgc
    67081 cgagatccag cgtattaccg ttgttgccgc ggatcacccc ctgcaccaga gcaaactgca
    67141 gcggttgggc aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac
    67201 cgttcagcgc agtgctgccg tcagtcaacg taccgtccca ggtgaaacta tggactccgg
    67261 cagtcagttc accgatatca atggtacgga cgaccgcgcc gtttttatcg gtgatggtgg
    67321 ccgtcacttt gtctgctgcc tgttgcaact caacaccaaa cggcgtggtc gtggtcacag
    67381 ccccttcttc actgccggtt cccgcaagaa cagtggtacc ggggatcatc acgccatgac
    67441 cgataaggtt actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga
    67501 gcgtggtatt gagtttttca atcccactga ccgtgctgat ttgtgccaat tgcgacgtca
    67561 gctcgttgtt ttccattgga ttggtcgggt cctggttttt cagctgcgcc accaacaaag
    67621 tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg
    67681 tactgacgcc ggtattcgtc ggatcggtgg tggttaccgc aatagacata gctttctcct
    67741 ttattgaccg agcgtaaggg ttttcagcat catgcttttc accgtgttga gcacttcaac
    67801 attggcctga taactgcgtg acgccgacat ggtgttaacc atctctccga cgacatcaac
    67861 gtttggcatt ttcacgtagc cctttgcatc cgccagcgga ttacccggtt cataaaccag
    67921 tttgtccggg gcctgacttt ctataacatc ggcaaccttt acgccgcctg tcgcagcacc
    67981 tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc catcgggacc
    68041 ggtcacgcta tcagcattcg ccagattact ggccgccacg ttcaggcgct gggactgggc
    68101 agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct
    68161 ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac acgctaaggc tcatctggta
    68221 ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc
    68281 aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg
    68341 cgccggaatg tgtttcgttg aggtcatcgt cagagcaacc acactggttg catcccgtcc
    68401 acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg
    68461 ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag
    68521 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    68581 atcaaaattc ggccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    68641 aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta
    68701 gaaatggctg gggacgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact
    68761 gttcagtccg ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc
    68821 ccaactcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcac ccaatctgct
    68881 accgccgtgc gatcagccat tgctttcgat gagcaataat tcccgtctgt ggggcaatgt
    68941 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    69001 aggaaattat gtggttgccg cgatgcccat tgtgcgtgga ggaaagctgg aagctagcaa
    69061 tgtcaaactg aaacgcggac ggctggatac cttgccaccg cgtacggtgc tggatatcaa
    69121 tcaacttgtt gatgccgtta gcctgcgcga tctatcaccc gatcaaccta tccagttaac
    69181 ccactttcgc caggcatggc gggtaaaagc gggacaacgt gtcaacgtga tcgccagcgg
    69241 tgatgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    69301 tgcgcgggtg cgcatgatat cgggacaagt ggtcagcggc gttgttgatg cagatgggaa
    69361 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    69421 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    69481 gaagccagta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa caaacacccg
    69541 ggcggcaaaa acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa
    69601 actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaagctggc
    69661 gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga
    69721 agcgcagcaa gacttgcaga gtaattgatc gtatgacgcg tcttgcagag atcctcgacc
    69781 agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct
    69841 ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc
    69901 tggcgacgct ggattacctc gaacagttac gcaggaaaga acccaataca gcaaatagcg
    69961 ttgatattag tcaacgctgg caggaaatta ctggcaaaac gcagcaacta cgccaactga
    70021 atcaacataa cggctggtta ctggaaggac agattgaacg caatcaacag gcgctggaaa
    70081 tgttgaaacc gcatcaggaa ccgacgctgt atggggcgaa cggtcagacc tcaacaaccc
    70141 atcgcggcag taaaaagatt tcgatctgaa gattaactcc ggcctgcact gcgggccgga
    70201 atgtatcgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt
    70261 gcagcgaaat acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taaacgccag
    70321 ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac
    70381 atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggggta
    70441 aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga
    70501 ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat
    70561 tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa
    70621 taaaagccag gagccaacac tttcactaca atcaggccaa tcaaacccac cgagtaggca
    70681 attaacgccc gctgggtcat cagcgcatca aaggcggtaa atttaccgta ctggaacagc
    70741 gaaacggtta acggaccgga aagaatgccc aacgcaaccg cactcggcag cgccaacagg
    70801 aaacaaagac gcagccccca gtccatcaaa cggttgtatt catcatgatt gccactggca
    70861 aaacttttcg acagcgacgg tagcaaaatg gtgccaagcg ccacgcccag cacaccggac
    70921 ggaaactcca ttaagcggtc ggcgtaatac atccacgaca ccgaaccgga agcaagaaac
    70981 gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc aaggattgcc
    71041 ggtcccatct gtttcaccac acgcattgct ccggcatcgt ggaagttaat gcgtggcaaa
    71101 accagcatgc cgatcttctt cagatgaggt agctgataca ccagctgcag gatgccgccg
    71161 accgtaacag cccaggccag cgccagcacc ggtggattaa agtacggcgc ggcaaacagc
    71221 gcaaaaccaa tcatgctgat attgagcaat gtcggggcaa acgccggaat tgagaagcgg
    71281 ttccaggtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga
    71341 aaggttatct ttagtagctg gctggtcagg gcaaatttgt cggcagtgtc agcgaagcca
    71401 ggcgcggtca ccatgatcac ccacggtgca gcgagcatgc cagcgaccgt cacaaccgcc
    71461 agcgcaagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc
    71521 tgcttgcttt tatattctgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca
    71581 aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc
    71641 cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac
    71701 atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag
    71761 acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt
    71821 atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat
    71881 cttgtcaacg atacgttgcg ccagcacggc ctgttcgccg gcggtttgcg gaactgtctg
    71941 attttgcacg cattcaatga agtgccgcgc acagccgaca aatccacgtt gctcgagcgt
    72001 actctgccaa ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc
    72061 gcgcatatcc gtaatgtcga tgagcgcacc atcagtcacg gcctgcacgg tttcacgctg
    72121 acttccggca cggcgatgca tacaggtggt aatctgcaaa ggaccagccg aaaaatggtg
    72181 ctcggcaaac agcatttcgc cggactcatt ggtcagcaac gtaccgcttt cgagagttgc
    72241 attgccgcca gacaaccaca gcgcggtatc aaccacatgc aaataatcat ccagcaatgt
    72301 gaaataaaga tcgtgtggcc cgacgctatt ggtacgatgt ttatccattc ttagcgagga
    72361 cgcggtggcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc
    72421 gaccatcaac gtcagttttt tccgcgccgc cagttccacc agccgttcag catcgcgcag
    72481 attttctgcc agcggtttat cgacacagac atgtaccccc gcattgagta acgtactgac
    72541 cacgtcaaag tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag
    72601 gctggataac gaatcagcat aaggaatgcg ccagctgtca caaattggca aggctttggc
    72661 gcgcgtaggc gaccacgcac cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa
    72721 ccacgctttt tgcgcgatgc cgcctaaccc cactacgccg atacgtaatt ttttcacttt
    72781 aatctcccag atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt
    72841 ccagggcttc gacgcgggcc tgtaaatcac cgtcagctgc gttcgacata tccgttaccg
    72901 ccggcggatt ctcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttacccg
    72961 gttcgcgggc cagacgcacc acaaaaggac catcttcgcg attcgccagt tgttccagcg
    73021 tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcgg ctgcgcagtt
    73081 cccccggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact tctgcggcgc
    73141 tcagtttcag atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat
    73201 taccaaaacc gctcaccgtg cgtagataat gacgtttgac cagattatcc agctgttcct
    73261 gcacttcaga ttcactcaga ttcataaccg gttcacggtt cgttttctga ttacaggccg
    73321 tgactacacc atttacggag agcgggtatt gctccggcgt ggtcacctgt ttttccagca
    73381 agcaaccaat cacgcgagct tccatgacag taagttgata tttcatgaga ttctccttag
    73441 cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca
    73501 tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa
    73561 tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg
    73621 cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat
    73681 ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccgcgaac aacattggaa
    73741 aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa gtaaaaagct
    73801 gaaccttgtt tatgaaattc gttaatcatc cctagcctgg cctgccagcc tgatggataa
    73861 cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa
    73921 taatccgcca gacgccaggc atcacgatca tacaccagac gcacgaccag tcggtctgtg
    73981 gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc
    74041 gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag
    74101 tccagaatgc cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac
    74161 gttttgataa aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa
    74221 tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtgtc gcaggcgagg
    74281 aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt
    74341 gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc
    74401 ggtattgccc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca
    74461 attgccgacc gctttggtgc caaaccgatg attgtcaccg gtatgctgat gcgtgccgcc
    74521 ggattcgcca caatgggtat cgcccacgaa ccgtggctac tgtggttttc atgcctgctt
    74581 tcgggactcg gcggcacgtt gtttgatcct ccgcgttcgg cgctggtggt gaaattaatc
    74641 cgtccacagc agcgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg
    74701 gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc
    74761 acaggggcag ttttgtttgt gctgtgtgca gcgttcaatg cgtggttgtt accggcatgg
    74821 aaactctcca ccgtgcgcac gcccgttcgc gaaggcatga cccgcgtgat gcgcgacaag
    74881 cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg
    74941 ctgatgctgc caattatggt caacgacgtg gctggcgcgc cctctgccgt taaatggatg
    75001 tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa
    75061 aagcattttc gtctggaaca ccggttaatg gctgggctgt tgataatgtc attaagcatg
    75121 atgccagtag gcatggtcag cggcctgcaa caacttttca ccctgatttg tctgttttat
    75181 atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcagacgca
    75241 agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt
    75301 ggttatatcg gtggcggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt
    75361 ccgtggatga tgctgggcat tattggcatc ttcactttcc ttgcgctggg ttggcagttt
    75421 agccagaaac gcgccgcgcg tcgtttgctt gaacgcgacg cctgatttga tactcccccc
    75481 tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac
    75541 atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc
    75601 cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt
    75661 caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggac
    75721 atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct
    75781 gccattgaag agtggctgcg caaggtcaat ggctacgcca acaaactgct gttgccgcgt
    75841 tttgccaaat cggcatttga tgagttttct actcctgccg cgcgcaaata tttcgtcgac
    75901 aagaaagagg ccagcgcggg taattttgcc gacctgctgg cccactctga cggtctgatt
    75961 aagaatatca gcgatgattt acgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg
    76021 aatggcgaac tttcggaaga tgatattcag ctatttccgc tcctgcgtaa tctgacgcta
    76081 gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag
    76141 acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt
    76201 attcgcgggt tgccgccgtt ttcagttttt tctgctggcg cgtttatcgc tggtgacaca
    76261 tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt
    76321 tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca
    76381 atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa
    76441 agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaacc tgaccagcca
    76501 aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa
    76561 ctccctgttc ggtagccaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt
    76621 tgataaagaa aaaggcgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca
    76681 accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttaaacc aggcattacg
    76741 caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagca atggcgaagc
    76801 aatggcgaag aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt
    76861 tactgattaa tcacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt
    76921 ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac
    76981 tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga
    77041 tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctatcgt
    77101 aatgcccaat caggctccgc ccgtgaccac cgttgaggct gccgtggcgt atcgccagcg
    77161 cattcttgac gccgtacctg ccgggcacga tttcaccccg ctgatgacct gttatttaac
    77221 agattcgctg gatcctaatg agctggatcg cggatttaac gaaggcgtgt tcaccgctgc
    77281 aaaactttat ccggcaaacg caaccactaa ctccagccac ggcgttacgt cagttgacgc
    77341 aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga
    77401 agtaacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat
    77461 ggaaccgctg cgccagcgcc tgaccgcgct gaaagtcgtt tttgagcata tcaccaccaa
    77521 agacgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca
    77581 gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg
    77641 tctacccatc ctcaaacgca atatccacca acaggcattg cgtgaactgg tcgccagcgg
    77701 ttttaatcgt gtattcctcg gaacggattc tgcgccacat gcacgtcatc gcaaagagag
    77761 cagctgcggc tgcgcgggct gtttcaacgc cccaaccgcg ctgggcagtt acgctaccgt
    77821 ctttgaagag atgaatgctt tgcagcactt tgaagcattc tgttctgtaa acggcccgca
    77881 gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt
    77941 tgctgaaagc atcgcactga ctgatgacac gctggtgcca ttcctcgccg gagaaacggt
    78001 acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tattttacct
    78061 gtataaataa ccagtgtatt caacaggggg ctattatgcg aattgaagtc accatagcga
    78121 aaacttctcc attgccagct ggggctattg acgccctggc tggcgaactt tcccgccgta
    78181 ttcagtatgc gtttcctgat aatgaaggcc acgtatcggt acgttatgcc gcagcaaata
    78241 atttatcggt tattggcgcg acaaaagaag ataaacagcg cattagcgaa attctccagg
    78301 aaacgtggga aagcgccgat gactggtttg tcagcgaata atatgcagtg atttttgttt
    78361 ttgccgggtc gccccggctt ttttatttta cttgcgaatt gtgagtacaa aatttctaca
    78421 cttcctcaaa ataattgcac aatatgctaa aaaaatatga atgacatgct gttttattgt
    78481 tcatataaat cttaaaagca gaaatttatc tgttcccccc tttaaaaatt cggtgaatac
    78541 ccttacttat tggtatactg aagttgcttc agataaaaac gcattgaacc tcgaataacg
    78601 ttgtctagta acacgaatta gggggccatg atggaaaaaa ataatgaagt cattcagact
    78661 catccgctcg tagggtggga catcagcacc gttgatagct atgatgcgct gatgttgcgt
    78721 ttgcactacc agaccccaaa taagtccgag caggaaggga ctgaagttgg tcagacgctc
    78781 tggttaacca ctgatgttgc cagacaattt atttcgatat tagaagcagg aatcgccaaa
    78841 attgaatccg gtgatttcca ggtaaacgag tatcggcgtc attaacatga catgaccatc
    78901 cacccaaaag gcacctcacc ggtgccttta ccatttctgg gttgtatatc tgcattcagt
    78961 taattactct gcttaaacca tgagagcaga gagaaaaaaa tgaaatacga tctcatcatt
    79021 attggcagcg gttccgtagg cgctgccgcc gggtattatg caacccgcgc cggtttaaac
    79081 gtgctaatga ccgacgccca tatgccaccg catcaacacg gcagccacca cggcgatacg
    79141 cgattaattc gccatgctta tggtgaaggt gaaaagtatg tcccgctggt cctccgcgcg
    79201 caaatgctgt gggatgaact ttcccgccac aacgaagatg atcccatttt tgtacgctct
    79261 ggtgtcatta acctcggtcc ggctgactcc gcatttctcg ccaacgtcgc ccacagcgcc
    79321 gaacagtggc aactcaacgt tgaaaagctc gacgcgcaag ggattatggc ccgctggcca
    79381 gaaatacgcg tcccggacaa ctacatcggt ttatttgaaa ctgattctgg ttttttgcgc
    79441 agcgaactgg cgattaaaac ctggatccaa ctggcgaagg aagcgggctg tgcgcagctg
    79501 ttcaactgcc cggtcaccgc aattcgtcat gacgatgatg gcgtaactat tgaaacggct
    79561 gacggtgtgt atcaggcgaa aaaagcgatt gtctgcgcgg gaacatgggt aaaagaccta
    79621 ctcccggagc tgcctgtcca gcccgtacgc aaagtatttg cctggtatca ggccgatggt
    79681 cgctatagcg tgaagaataa attcccggcg tttaccggtg aactgcccaa tggcgatcaa
    79741 tattatggtt ttccggcaga aaacgacgcg ttgaagattg gcaaacataa cggaggccag
    79801 gttatccatt cagcggatga acgtgttccg tttgcggaag tggtcagcga tggttcggaa
    79861 gccttcccgt tcttgcgcaa tgtgttgccg ggtatcggtt gctgcctgta cggcgctgcc
    79921 tgcacctatg ataattcgcc tgacgaagat tttattatcg ataccctacc cggccacgat
    79981 aatacactgc tcattaccgg cctgagtggg cacggtttta aatttgcgtc agttttaggg
    80041 gaaatagctg ccgattttgc gcaagacaaa aaaagcgatt ttgatttgac gccattcagg
    80101 ctttcccgct tccaataatc atcattacgg cctccagaat gaggccgttt ttacaggagt
    80161 atttaatgcg tcgactgttg cactatctca tcaataatat tcgcgagcat ctgatgcttt
    80221 atctttttct ctggggattg ttggcaatta tggatctgat ctatgtgttt tatttttagt
    80281 tttccgcact gacataaact gacaaatatt taatctccct ttcatttagc gtgaaaaatt
    80341 gaatgag
//
LOCUS       DAZGJT010000005        76773 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZGJT010000005 DAZGJT010000000
VERSION     DAZGJT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.005"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(55..131)
                     /locus_tag="ACHKFK_000357"
     tRNA            complement(55..131)
                     /locus_tag="ACHKFK_000357"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Arg,seq:acg)
     gene            complement(135..227)
                     /locus_tag="ACHKFK_000358"
     tRNA            complement(135..227)
                     /locus_tag="ACHKFK_000358"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Ser,seq:gct)
     gene            complement(542..727)
                     /gene="csrA"
                     /locus_tag="ACHKFK_000359"
     CDS             complement(542..727)
                     /gene="csrA"
                     /locus_tag="ACHKFK_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO9050785.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(962..3592)
                     /gene="alaS"
                     /locus_tag="ACHKFK_000360"
     CDS             complement(962..3592)
                     /gene="alaS"
                     /locus_tag="ACHKFK_000360"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO9050786.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAIATVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3721..4221)
                     /gene="recX"
                     /locus_tag="ACHKFK_000361"
     CDS             complement(3721..4221)
                     /gene="recX"
                     /locus_tag="ACHKFK_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO9050787.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4290..5351)
                     /gene="recA"
                     /locus_tag="ACHKFK_000362"
     CDS             complement(4290..5351)
                     /gene="recA"
                     /locus_tag="ACHKFK_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO9050788.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5431..5928)
                     /gene="pncC"
                     /locus_tag="ACHKFK_000363"
     CDS             complement(5431..5928)
                     /gene="pncC"
                     /locus_tag="ACHKFK_000363"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO9050789.1"
                     /translation="MTDSELMQLSEQVGQALKVRGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6073..7158)
                     /gene="mltB"
                     /locus_tag="ACHKFK_000364"
     CDS             complement(6073..7158)
                     /gene="mltB"
                     /locus_tag="ACHKFK_000364"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO9050790.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7415..7978
                     /gene="srlA"
                     /locus_tag="ACHKFK_000365"
                     /gene_synonym="gutA"
     CDS             7415..7978
                     /gene="srlA"
                     /locus_tag="ACHKFK_000365"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO9050791.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVRLAGATS"
     gene            7975..8922
                     /gene="srlE"
                     /locus_tag="ACHKFK_000366"
                     /gene_synonym="gutE"
     CDS             7975..8922
                     /gene="srlE"
                     /locus_tag="ACHKFK_000366"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO9050792.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGLGNIPPHLALPALFAINAQAACDFIPVGLSL
                     AEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8933..9304
                     /gene="srlB"
                     /locus_tag="ACHKFK_000367"
                     /gene_synonym="gutB"
     CDS             8933..9304
                     /gene="srlB"
                     /locus_tag="ACHKFK_000367"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO9050793.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGRHRYPVTAVGSVAEDNLRELGHVTLRFDGLSEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9308..10087
                     /gene="srlD"
                     /locus_tag="ACHKFK_000368"
     CDS             9308..10087
                     /gene="srlD"
                     /locus_tag="ACHKFK_000368"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO9050794.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGTAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPEQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10193..10552
                     /gene="gutM"
                     /locus_tag="ACHKFK_000369"
     CDS             10193..10552
                     /gene="gutM"
                     /locus_tag="ACHKFK_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO9050795.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHVGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10619..11392
                     /gene="srlR"
                     /locus_tag="ACHKFK_000370"
                     /gene_synonym="gutR"
     CDS             10619..11392
                     /gene="srlR"
                     /locus_tag="ACHKFK_000370"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO9050796.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVGFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11385..12350
                     /gene="gutQ"
                     /locus_tag="ACHKFK_000371"
     CDS             11385..12350
                     /gene="gutQ"
                     /locus_tag="ACHKFK_000371"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO9050797.1"
                     /translation="MSEALLNAGRQTLMLELQEASHLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDDQRLVKGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12347..13861)
                     /gene="norR"
                     /locus_tag="ACHKFK_000372"
     CDS             complement(12347..13861)
                     /gene="norR"
                     /locus_tag="ACHKFK_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO9050798.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGEAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYNFPGNVRELEHAIHRAVVLARATRSGDEVILEAQHFAFPEVTLPPPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14048..15487
                     /gene="norV"
                     /locus_tag="ACHKFK_000373"
     CDS             14048..15487
                     /gene="norV"
                     /locus_tag="ACHKFK_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO9050799.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIVYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVEEETSAATTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15484..16617
                     /gene="norW"
                     /locus_tag="ACHKFK_000374"
     CDS             15484..16617
                     /gene="norW"
                     /locus_tag="ACHKFK_000374"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO9050800.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRCIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INSQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WHIYTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLSM"
     gene            complement(16839..19091)
                     /gene="hypF"
                     /locus_tag="ACHKFK_000375"
     CDS             complement(16839..19091)
                     /gene="hypF"
                     /locus_tag="ACHKFK_000375"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO9050801.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGVMNTQI
                     VPDAATCPACLAEMNTPGERRWRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYCDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKQLAVMLPTAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHLWPLDGGDVIALTLDGIGMGE
                     NCALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAAPCHRVTHPVTMPLVDNQLDLATFWQQWLSWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIVAA
                     RWLAGEVQNG"
     gene            complement(19244..19771)
                     /gene="hydN"
                     /locus_tag="ACHKFK_000376"
     CDS             complement(19244..19771)
                     /gene="hydN"
                     /locus_tag="ACHKFK_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO9050802.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            20156..20584
                     /locus_tag="ACHKFK_000377"
     CDS             20156..20584
                     /locus_tag="ACHKFK_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050803.1"
                     /translation="MGSWGMEALESDEGLDLINWVEEQLQDDSTFDAESIVQRLSQHE
                     DLFGFQGDEEFLYDNNVIGLVELIIQKAAGEKITSSKQIDQLDSYRLTSIFSKKLQGR
                     LQTIDDTHEWIMLFEGRAREKAKAYLIEITDKLRVVKTTA"
     gene            complement(20676..21095)
                     /locus_tag="ACHKFK_000378"
     CDS             complement(20676..21095)
                     /locus_tag="ACHKFK_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9050804.1"
                     /translation="MTANAVRAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLTSRIAEYENNNEKFAEFDKAVAAMPVGAALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(21092..21403)
                     /locus_tag="ACHKFK_000379"
     CDS             complement(21092..21403)
                     /locus_tag="ACHKFK_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFO9050805.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(21566..22321)
                     /locus_tag="ACHKFK_000380"
     CDS             complement(21566..22321)
                     /locus_tag="ACHKFK_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050806.1"
                     /translation="MCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLLTMPYDA
                     DQNWHFRKVGVAGVTLEKMPNEDDYWRLTAKDRAGKSWFVPVGVLQNMAGNAQFYRAD
                     LDRNGIQDLVIWRGVSGNGLAPSSFLILMTFNQQGRPCVFQVDGFYTANETGVDDLLD
                     LQGNGHTQLLDMQFDSGYWITSLYQVKEAKWQRVHGWFGKLSYPALTRFTYTSNRKLV
                     IKPVAGRNPQTKDLALTQRCLIKGDVFDGVNQN"
     gene            complement(22364..22834)
                     /gene="hycI"
                     /locus_tag="ACHKFK_000381"
     CDS             complement(22364..22834)
                     /gene="hycI"
                     /locus_tag="ACHKFK_000381"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO9050807.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE"
     gene            complement(22827..23237)
                     /gene="hycH"
                     /locus_tag="ACHKFK_000382"
     CDS             complement(22827..23237)
                     /gene="hycH"
                     /locus_tag="ACHKFK_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO9050808.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(23234..24001)
                     /gene="hycG"
                     /locus_tag="ACHKFK_000383"
     CDS             complement(23234..24001)
                     /gene="hycG"
                     /locus_tag="ACHKFK_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO9050809.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIDSMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(24001..24543)
                     /gene="hycF"
                     /locus_tag="ACHKFK_000384"
     CDS             complement(24001..24543)
                     /gene="hycF"
                     /locus_tag="ACHKFK_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO9050810.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWQFDLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(24553..26262)
                     /gene="hycE"
                     /locus_tag="ACHKFK_000385"
     CDS             complement(24553..26262)
                     /gene="hycE"
                     /locus_tag="ACHKFK_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO9050811.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(26280..27203)
                     /gene="hycD"
                     /locus_tag="ACHKFK_000386"
     CDS             complement(26280..27203)
                     /gene="hycD"
                     /locus_tag="ACHKFK_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO9050812.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(27206..29032)
                     /gene="hycC"
                     /locus_tag="ACHKFK_000387"
     CDS             complement(27206..29032)
                     /gene="hycC"
                     /locus_tag="ACHKFK_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO9050813.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEIMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLINHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     DCPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(29029..29640)
                     /gene="hycB"
                     /locus_tag="ACHKFK_000388"
     CDS             complement(29029..29640)
                     /gene="hycB"
                     /locus_tag="ACHKFK_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO9050814.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCATVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(29765..30226)
                     /gene="hycA"
                     /locus_tag="ACHKFK_000389"
     CDS             complement(29765..30226)
                     /gene="hycA"
                     /locus_tag="ACHKFK_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO9050815.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            30438..30788
                     /gene="hypA"
                     /locus_tag="ACHKFK_000390"
     CDS             30438..30788
                     /gene="hypA"
                     /locus_tag="ACHKFK_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9050816.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            30792..31664
                     /gene="hypB"
                     /locus_tag="ACHKFK_000391"
     CDS             30792..31664
                     /gene="hypB"
                     /locus_tag="ACHKFK_000391"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO9050817.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            31655..31927
                     /gene="hypC"
                     /locus_tag="ACHKFK_000392"
     CDS             31655..31927
                     /gene="hypC"
                     /locus_tag="ACHKFK_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO9050818.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            31927..33048
                     /gene="hypD"
                     /locus_tag="ACHKFK_000393"
     CDS             31927..33048
                     /gene="hypD"
                     /locus_tag="ACHKFK_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO9050819.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            33045..34055
                     /gene="hypE"
                     /locus_tag="ACHKFK_000394"
     CDS             33045..34055
                     /gene="hypE"
                     /locus_tag="ACHKFK_000394"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO9050820.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHRG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELSEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            34129..36207
                     /gene="flhA"
                     /locus_tag="ACHKFK_000395"
     CDS             34129..36207
                     /gene="flhA"
                     /locus_tag="ACHKFK_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO9050821.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLATGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFNTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRILSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            36252..36680
                     /locus_tag="ACHKFK_000396"
     CDS             36252..36680
                     /locus_tag="ACHKFK_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HFO9050822.1"
                     /translation="MAVSARNQLTGTVSAVAMGAVNDEVELTLAGGAKLVAIVTHSSQ
                     QALGLAKGKEAIALIKAPWVTLATEDCGLKFSARNQFAGSVSTITEGAVNATVHIKTD
                     AGFEIVAVVTNESQDEMKLTTGSRVIALIKASAILIATKA"
     gene            complement(36719..37063)
                     /gene="ygbA"
                     /locus_tag="ACHKFK_000397"
     CDS             complement(36719..37063)
                     /gene="ygbA"
                     /locus_tag="ACHKFK_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO9050823.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFAYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKK"
     gene            37349..39910
                     /gene="mutS"
                     /locus_tag="ACHKFK_000398"
     CDS             37349..39910
                     /gene="mutS"
                     /locus_tag="ACHKFK_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO9050824.1"
                     /translation="MSTIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQSESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMERQQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLENVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            40016..40672
                     /gene="pphB"
                     /locus_tag="ACHKFK_000399"
     CDS             40016..40672
                     /gene="pphB"
                     /locus_tag="ACHKFK_000399"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9050825.1"
                     /translation="MPSTRYQKINAHHYRHIWTVGDIHGDYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLNVLRLLNQPWFISVKGNHEAMALDAFETGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDTIKYVIAHADYPGKEYQFGKEIAESELL
                     WPVDRVQKSLNSELQKINGADFFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIK
                     "
     gene            complement(40723..41490)
                     /gene="ygbI"
                     /locus_tag="ACHKFK_000400"
     CDS             complement(40723..41490)
                     /gene="ygbI"
                     /locus_tag="ACHKFK_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO9050826.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGRCIYLDAGT
                     TTLAIAQHLIHMEPLTVVTNDFVIADYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKLDLSLLVAKNE"
     gene            41686..42594
                     /gene="ltnD"
                     /locus_tag="ACHKFK_000401"
     CDS             41686..42594
                     /gene="ltnD"
                     /locus_tag="ACHKFK_000401"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO9050827.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNATQVKQVLFGEKGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFGLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGSEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            42591..43853
                     /gene="otnK"
                     /locus_tag="ACHKFK_000402"
     CDS             42591..43853
                     /gene="otnK"
                     /locus_tag="ACHKFK_000402"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO9050828.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVLEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVES
                     QSTGRCGVVSAHVFEQGVKAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSIETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            43850..44488
                     /gene="ygbL"
                     /locus_tag="ACHKFK_000403"
     CDS             43850..44488
                     /gene="ygbL"
                     /locus_tag="ACHKFK_000403"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO9050829.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNIPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            44493..45269
                     /gene="ygbM"
                     /locus_tag="ACHKFK_000404"
     CDS             44493..45269
                     /gene="ygbM"
                     /locus_tag="ACHKFK_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO9050830.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSPQQ
                     IQQQLVQNHLTLALFNTAPGDINAGEWGLSALPGREHEARADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            45358..46722
                     /gene="ygbN"
                     /locus_tag="ACHKFK_000405"
     CDS             45358..46722
                     /gene="ygbN"
                     /locus_tag="ACHKFK_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO9050831.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WLVI"
     gene            complement(46865..47908)
                     /locus_tag="ACHKFK_000406"
     CDS             complement(46865..47908)
                     /locus_tag="ACHKFK_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFO9050832.1"
                     /translation="MQGIKIYTILASSLISGSIFAQTEISTANSSTVNASYVESSVKK
                     IISPSDKLNNLFERNMSQPYILQKIGERTYYVQRYFYSTTFYVGDKGVLLFDAPEGRG
                     KYLLQAIRDVTPLPVTALVYSHYHVDHIGDSPFWNDEAKKEGVNLRIIASKATAEKMQ
                     FMNSRLPVATQVLSKKDDQFKFEKQTIELHRFVKAGHTDDHSVWLLKQEKVAHSPDLL
                     NPDQLPMMGFAVSDTLVYHDSNLRQVEMLDWKYFIGGHGNIGSHDDFKFQRQFLNDLR
                     DTTIKVRKEESFGKFMNKTANNHADFARAQREAIIKKVTEVLRPKYGHMYGYDASMPA
                     NIEIAIRLVGSYY"
     gene            48133..48684
                     /locus_tag="ACHKFK_000407"
     CDS             48133..48684
                     /locus_tag="ACHKFK_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFO9050833.1"
                     /translation="MQNIAVVYYSGYGHTKLVAEKVAQGAHADLISIDNEGNITEQEW
                     EKLDAADAIIFGAPTYMGGAPWQFKKFADASSKAWFTRKWQDKVFGGFTNSASLNGDK
                     QVTLIYLQTLASQHGGLWGSLGQAPANVLASTREDVNNLGGSVGLLVQSPADAGADQI
                     PTGDLDTAVKYGERVATITARLK"
     gene            complement(48855..49847)
                     /gene="rpoS"
                     /locus_tag="ACHKFK_000408"
     CDS             complement(48855..49847)
                     /gene="rpoS"
                     /locus_tag="ACHKFK_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO9050834.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(49910..51004)
                     /gene="nlpD"
                     /locus_tag="ACHKFK_000409"
     CDS             complement(49910..51004)
                     /gene="nlpD"
                     /locus_tag="ACHKFK_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO9050835.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPTATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(51189..51815)
                     /gene="pcm"
                     /locus_tag="ACHKFK_000410"
     CDS             complement(51189..51815)
                     /gene="pcm"
                     /locus_tag="ACHKFK_000410"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO9050836.1"
                     /translation="MVSRRVQALLDQLRAQGIQDELVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(51809..52570)
                     /gene="surE"
                     /locus_tag="ACHKFK_000411"
     CDS             complement(51809..52570)
                     /gene="surE"
                     /locus_tag="ACHKFK_000411"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO9050837.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPHPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(52551..53600)
                     /gene="truD"
                     /locus_tag="ACHKFK_000412"
     CDS             complement(52551..53600)
                     /gene="truD"
                     /locus_tag="ACHKFK_000412"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO9050838.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKVLMITAALPGSGEWGTQREALAFEQ
                     AAVAEETELQTLLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(53597..54076)
                     /gene="ispF"
                     /locus_tag="ACHKFK_000413"
     CDS             complement(53597..54076)
                     /gene="ispF"
                     /locus_tag="ACHKFK_000413"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO9050839.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYALGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(54076..54786)
                     /gene="ispD"
                     /locus_tag="ACHKFK_000414"
     CDS             complement(54076..54786)
                     /gene="ispD"
                     /locus_tag="ACHKFK_000414"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO9050840.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPRITVVDGGEERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(54805..55116)
                     /gene="ftsB"
                     /locus_tag="ACHKFK_000415"
     CDS             complement(54805..55116)
                     /gene="ftsB"
                     /locus_tag="ACHKFK_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO9050841.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNNDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(55309..55632)
                     /gene="ygbE"
                     /locus_tag="ACHKFK_000416"
     CDS             complement(55309..55632)
                     /gene="ygbE"
                     /locus_tag="ACHKFK_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO9050842.1"
                     /translation="MRNSHNITLINNDSLTEVEDTTWSLPGAVVGFLSWLCALAIPML
                     IYGSNTLLFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLATVGIMFGALF
                     MWLLG"
     gene            complement(55682..56287)
                     /gene="cysC"
                     /locus_tag="ACHKFK_000417"
     CDS             complement(55682..56287)
                     /gene="cysC"
                     /locus_tag="ACHKFK_000417"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO9050843.1"
                     /translation="MALHDENVVWHSHPVTPQQREQHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(56287..57714)
                     /gene="cysN"
                     /locus_tag="ACHKFK_000418"
     CDS             complement(56287..57714)
                     /gene="cysN"
                     /locus_tag="ACHKFK_000418"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO9050844.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIHYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(57716..58624)
                     /gene="cysD"
                     /locus_tag="ACHKFK_000419"
     CDS             complement(57716..58624)
                     /gene="cysD"
                     /locus_tag="ACHKFK_000419"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO9050845.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            58876..59928
                     /gene="iap"
                     /locus_tag="ACHKFK_000420"
     CDS             58876..59928
                     /gene="iap"
                     /locus_tag="ACHKFK_000420"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO9050846.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAECLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAILMGLK"
     gene            complement(60039..60191)
                     /locus_tag="ACHKFK_000421"
     CDS             complement(60039..60191)
                     /locus_tag="ACHKFK_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9050847.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(60454..61188)
                     /gene="cysH"
                     /locus_tag="ACHKFK_000422"
     CDS             complement(60454..61188)
                     /gene="cysH"
                     /locus_tag="ACHKFK_000422"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9050848.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAQLEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGIAEEETRFFGLKRECGLHEG"
     gene            complement(61262..62974)
                     /gene="cysI"
                     /locus_tag="ACHKFK_000423"
     CDS             complement(61262..62974)
                     /gene="cysI"
                     /locus_tag="ACHKFK_000423"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO9050849.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREVGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(62974..64773)
                     /gene="cysJ"
                     /locus_tag="ACHKFK_000424"
     CDS             complement(62974..64773)
                     /gene="cysJ"
                     /locus_tag="ACHKFK_000424"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO9050850.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGTVNEIHTSPYSKDAPLAASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPTN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKIYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            65092..65454
                     /gene="queD"
                     /locus_tag="ACHKFK_000425"
     CDS             65092..65454
                     /gene="queD"
                     /locus_tag="ACHKFK_000425"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO9050851.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPQGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            65532..66803
                     /gene="ygcN"
                     /locus_tag="ACHKFK_000426"
     CDS             65532..66803
                     /gene="ygcN"
                     /locus_tag="ACHKFK_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9050852.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLLPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLTLEPSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLARFKTHPAVRPLIKNTESLEYGAHLVP
                     EGGLRSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFPLYHHNVERSLLWDVLQRYQHVPALLQRPGWYRVWPGLMQDISRDLWDQGNTP
                     VPPLRQLLWRHLRRHGLWHLTGDVIRSLRCL"
     gene            66794..67054
                     /gene="ygcO"
                     /locus_tag="ACHKFK_000427"
     CDS             66794..67054
                     /gene="ygcO"
                     /locus_tag="ACHKFK_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9050853.1"
                     /translation="MSVARNLWRAADAPHIVPADSVERQTAQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            67071..67646
                     /gene="ygcP"
                     /locus_tag="ACHKFK_000428"
     CDS             67071..67646
                     /gene="ygcP"
                     /locus_tag="ACHKFK_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO9050854.1"
                     /translation="MSLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEAYGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVTQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(67794..68654)
                     /gene="ygcQ"
                     /locus_tag="ACHKFK_000429"
     CDS             complement(67794..68654)
                     /gene="ygcQ"
                     /locus_tag="ACHKFK_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9050855.1"
                     /translation="MNIAIVTINQEHAAMAGWLAAQDFSGSTLTHWQIEPQPMVAEQV
                     LDALVEQWQRTPAEVVLFPPGAFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGADKNATLPFGMQQLNIVPGALPDWLISVENLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKMGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(68651..69430)
                     /gene="ygcR"
                     /locus_tag="ACHKFK_000430"
     CDS             complement(68651..69430)
                     /gene="ygcR"
                     /locus_tag="ACHKFK_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO9050856.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWLAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLVQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQYQNPLDLIITGCQSSEGQNGQTPFLLAEMLVWPCFTQVERFTLDAPFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMATGKAEITRETVAAEAPA
                     IQCLQLARPEQRRRAALIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(69408..70892)
                     /gene="ygcS"
                     /locus_tag="ACHKFK_000431"
     CDS             complement(69408..70892)
                     /gene="ygcS"
                     /locus_tag="ACHKFK_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9050857.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFMLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISESPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLASAALLALLITLLRWGTPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGD
                     EVATATHKHIKTLFSSRYWRRTAFNSVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTA
                     SLMLNALLIVGALLGLVLTHLLAHRRFLLGSFLLLAATLVVMACLPSGSSLTLLLFVL
                     FSTTISAVSNLVGILPAESFPTDIRSLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQA
                     TLLLLAAVLLVGFVVTWLWAPETKALPLVAAGNVGGANEHTVSV"
     gene            complement(70914..72368)
                     /gene="ygcU"
                     /locus_tag="ACHKFK_000432"
     CDS             complement(70914..72368)
                     /gene="ygcU"
                     /locus_tag="ACHKFK_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO9050858.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYVLEDMKTGFNILREIMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRMAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPYADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(72438..73223)
                     /gene="ygcW"
                     /locus_tag="ACHKFK_000433"
     CDS             complement(72438..73223)
                     /gene="ygcW"
                     /locus_tag="ACHKFK_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9050859.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     VFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            73542..74819
                     /gene="yqcE"
                     /locus_tag="ACHKFK_000434"
     CDS             73542..74819
                     /gene="yqcE"
                     /locus_tag="ACHKFK_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO9050860.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIIFYAPSGVIADKFSHRKMITSAMIITGLLGLIMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVVQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGYWQDTLPAAEAYRNMWL
                     MGMAALGMVIIFTFLLFQKIRTADSAPAMASSK"
     gene            74846..76324
                     /gene="ygcE"
                     /locus_tag="ACHKFK_000435"
     CDS             74846..76324
                     /gene="ygcE"
                     /locus_tag="ACHKFK_000435"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO9050861.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFASHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSEN
                     AAVMEKFNIPRQMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDAKAQDLSPEDLLNKKASCVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCHEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYTTAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNADSIQSWSNA"
BASE COUNT        18434 a        20619 c        19486 g        18234 t
ORIGIN      
        1 ttactttact gcagattttt tgataccgcc actaaagtcg tatcaagtaa gagatggtgc
       61 atccgggagg attcgaacct ccgaccgctc ggttcgtagc cgagtactct atccagctga
      121 gctacggatg caaatggcgg tgaggcgggg attcgaaccc cggatgcagc ttttgaccgc
      181 atactccctt agcaggggag cgccttcagc ctctcggcca cctcaccaca cgcctcttac
      241 gagtgcttcg aagaacttgt ttatcgctca tcgtcgctgc gtggcgcaca tattactttc
      301 tgagacttat aagtcaaaca atttttccca cacttttatc gtttgcacac ttcacgctca
      361 attagtctgt aaaaacgtca aaaagagtgt tttatcaaca gaaaaacgga ggtctgacag
      421 atagcagtaa tgcaaaaaat ggagacttaa gttgaatgaa cgggagtaaa gcgaaaagac
      481 aatggagtga aagagaaatt ttgagggtgc gtctcaccga taaagatgag acgcggaaag
      541 attagtaact ggactgctgg gatttttcag cctggatacg ctggtagatc tcttcacggt
      601 gaacagaaac ttccttcggg gcatttacgc caatacgtac ctggttgccc tttaccccta
      661 aaactgtcac ggtgacctca tccccaatca tgagggtctc accaactcga cgagtcagaa
      721 tcagcattct ttgctccttg aaagattaaa agagtcgggt ctctctgtat cccggcatta
      781 tccatcatat aacgccaaaa agtaagcaat gacaaacaca ttacatctaa gcagtcatgg
      841 cattacattc tgttaaacct aagtttagcc gatatacaca acttcaacct gactttatcg
      901 ttgtcgatag cgttaatgcg aatgccgtga agcgagtcca cggcattgcc tgacgcttat
      961 attattgcaa tttcgcgctg acccagcctt tcacactggc taacgctgca ggtaaggccg
     1021 cagcatccgt accaccggct tgcgccatgt caggacgtcc accacccttg ccgcccacct
     1081 gctgagcgac cataccaatc agttcccctg ctttcacacg atctgtgacg tccttagata
     1141 cgcctgcaat cagagaaacc ttaccttcgg ctaccgttgc cagcacgata attgtcgacc
     1201 ccagctgatt ttttaaatcg tcaaccatgg tacgcaacat tttcggctca acaccgctaa
     1261 gctcgctaac caacagctta acaccattaa catcaattgc cttactggaa agatttgcgc
     1321 tctcctgtgc ggcagcttgt tctttaagct gttgtaactc tttttccagc tgacgcgtac
     1381 gttccagtac tgaacgcact ttatcagcca gattattgct atcgcctttc agcagatgcg
     1441 cgacttcgct taagcgatcg ctgtctgcat gaacggtggc gatagcgcct tctccggtca
     1501 ccgcttcgat acgacgaacg cctgcagcag tacccgattc agagatgatg cggaacagac
     1561 caatatcacc agtgcggctg gcgtgagtac cgccacacag ctcggtggag aaatcgccca
     1621 tgctcagcac gcgtacgcgc tcatcatact tctcgccaaa cagcgccatc gcacctttcg
     1681 ctttcgccgc ttcgagatcc atgatgttgg tttcgatcgg caagttacgg cgaatctgtg
     1741 cgttcaccag gtcttcgacc gcacgaatct cttctggttt catcgcttcg ttgtgtgaga
     1801 agtcgaagcg cagcactttg tcgttaacca gtgaaccttt ctgcgataca tgagtaccca
     1861 gaacctggcg cagcgcagcg tgcatcaggt gcgttgcgga gtgattcaga cgaatacggg
     1921 cgcgacgagc ctcatcaaca tcagcctgca cagcgtcgcc cactttcaga gaacccgcag
     1981 caagtttacc gatgtggcca atcgcctggc cgtatttctg cgtatcttcc accgcgaagg
     2041 agaagttagc gcctttcagt tcgcctttat caccaacctg accgccggat tccgcataga
     2101 atggcgtttg atccagcacg accacagctt cctggcctgc attgatggca tcaaccgctt
     2161 taccatcaac aaacagcgcg gtcactttgc cgttcagttc cagatggtca tagcctttaa
     2221 attcagatgc actgtcaaca cggatcattg cgttgtaatc ggcaccaaag ccgctggctt
     2281 cgcgcgcacg acgacgctgc tcttccattg ctgcttcaaa accagcttcg tcaactttga
     2341 tgttgcgctc acgacaaaca tcagccgtca ggtcaaccgg gaagccgtag gtgtcgtaca
     2401 ggcggaaagc agtttcacca tccagcgtat caccagaaag ttttgccagc tcttcatcca
     2461 gcagcgccag accgcgctcc agagtacggg caaactgctc ttcttcagtc ttcagcacct
     2521 gctcaacctg cgcctgctgg cgtttcaggt cttcacccgc agagcccata acgtcgatca
     2581 gcggaccaac cagtttgtag aagaaggttt ctttcgcgcc gagcatattg ccgtgacgca
     2641 ctgcgcgacg aatgatacga cgcagtacat aaccacggtt ttcgttggac ggcattacgc
     2701 catccgcgat caggaacgca caagaacgaa tgtggtcagc gattacgcgc agcgatttat
     2761 tgctcagatc ggtcgcgcca gtgactttcg ctaccgcctg gatcaacgtg cggaacaggt
     2821 cgatgtcata gttagagtta acgtgttgca gcaccgcagc aatacgctcc agacccatac
     2881 cggtatctac agacggtttc ggcagcggtt ccatcgtgcc atcggcctgg cggttgaact
     2941 gcatgaagac gatgttccag atctcaatgt agcggtcgcc gtcttcttcc ggacttcccg
     3001 gagggccgcc ccaaatgtgg tcgccgtgat cgtagaagat ttcggtgcac gggccgcacg
     3061 gaccagtgtc acccatctgc cagaagttgt cagatgcgta tggcgcacct ttgttatcgc
     3121 cgatgcgaat aatacgttcg cgcgggatcc ctacttcttt ttcccagatt tcgtaggctt
     3181 cgtcgtcgct ttcatagacg gtaacccaca gacgctcttt cggcagggca aaccattttt
     3241 cgctggtcag cagttcccat gcaaactgaa tggcatcgtg tttgaaatag tcgccgaagc
     3301 tgaagttgcc cagcatttcg aagaaggtat ggtgacgcgc ggtgtaaccg acgttttcca
     3361 ggtcgttgtg tttaccaccc gcacgcacgc agcgttggga agtggtagcg cgggaataat
     3421 tacgcttgtc gagcccaagg aacacatcct tgaactggtt catcccggcg ttggtaaaca
     3481 acagagttgg gtcgttatgg ggtaccaggg agctgctggc aactacctga tgtcccttac
     3541 tatggaaaaa gtcgagaaac gcctgacgga tctcagcggt gctcttgctc ataattatcc
     3601 tgaaatcaag ctaacgaaat atcgctacca gccgttgcgt gtcgttaacc gcccggctgg
     3661 taactgaaaa gtgggaataa gataagtttt cttgactggg aagtaaaatc ccgtatgcgt
     3721 tcagtcggca aaatttcgcc aaatatcctg gatatcttcc atcagatagc cacgatagag
     3781 cagaaaacgc tggatcttaa ctttttctga aaagacagtt ggcaaaggtt cgccatattt
     3841 tcgcgtcgcc tgatcgcgcg ccagtgcgca ccagtcgatg tcacattcac gcatcgcttt
     3901 ttctgtcgct tcgcgggaaa tacctttctg attcagttcc tgacgaatac gcgcaggtcc
     3961 ataacctttg cggctacggc tggcgataaa gcgcgcaaca aatcggctgt catcaagata
     4021 gccatgttca tggcaccagg caataacgcg ctcgtaatct tctgccgtgg catcaatctc
     4081 ttccgggcca tttttgccca taatcggtgc cgcgagttta cgtcgcagtt cttgctcact
     4141 gtgatcgcgc accgccagaa tgcgtaccgc acgatccaac aggcgagcat atgccgggcg
     4201 acgggatgtt gattctgtca tggcatatcc ttacaactta aaaaagcaaa agggccgcag
     4261 atgcgaccct tgtgtatcaa acaagacgat taaaaatctt cgttagtttc tgctacgcct
     4321 tcgctgtcat ctacagagaa atccggcgtt gagttcgggt tgctcagcag caactcacgt
     4381 actttcttct cgatctcttt cgcggtttct gggttatctt tcagccaggc agtcgcattc
     4441 gctttaccct gaccgatctt ctcacctttg tagctgtacc acgcgcctgc tttctcgata
     4501 agcttctctt tcacgcccag gtcaaccagc tcgccgtaga agttgatacc ttcgccgtag
     4561 aggatctgga attcagcctg tttaaacggc gcagcgattt tgttcttcac cactttcacg
     4621 cgggtttcgc tacccaccac gttttcgccc tctttcaccg cgccgatacg acggatgtcg
     4681 agacgaacag aggcgtagaa tttcagcgcg ttaccaccgg tagtggtttc cgggttacca
     4741 aacatcacac caattttcat acggatctgg ttgatgaaga tcagcagcgt gttggactgc
     4801 ttcaggttac ccgccagctt acgcatcgcc tggctcatca tacgtgccgc aaggcccatg
     4861 tgagagtcgc cgatttcgcc ttcgatttcc gctttcggcg tcagtgccgc cacggagtca
     4921 acgacgataa cgtctactgc gccagaacgt gccagggcgt cacagatttc cagtgcctgc
     4981 tcgccggtat ccggctggga gcacagcagg ttgtcgatat cgacgcccag tttacgtgcg
     5041 tagattgggt ccagcgcgtg ttcagcatcg ataaacgcac aggttttacc ttcgcgctgc
     5101 gctgcggcga tcacctgcaa cgtcagcgtg gttttaccgg aagattctgg tccgtagatt
     5161 tcgacgatac ggcccatcgg cagaccacct gccccaagcg cgatatccag tgaaagcgaa
     5221 ccggtagaga tggtttccac atccatggaa cggtcttcac ccaggcgcat gatggagcct
     5281 ttaccaaatt gtttctcaat ctggcccagt gctgccgcca acgctttctg tttgttttcg
     5341 tcgatagcca tttttactcc tgtcatgccg ggtaataccg gatagtcaat atgttctgtt
     5401 gaagcaatta tactgtatgc tcatacagta tcaagtgttt tgtagaaatt gttgccacaa
     5461 ggtctgcaat gcatacgcag tagcctgacg acgcaccgca tcacggtcac cgctgaagca
     5521 ttcccgccgg gtaatgcctt caccacgggc agtggcaaaa gcaaaccaga cggtgccgac
     5581 aggcttctct tcactgccgc catccggccc ggcgatacca ctaatagaca cggcgtaatc
     5641 agcacgagcc gctttcagtg cgcctatcgc catttccacc acgacgggtt cactcaccgc
     5701 gccatgctgc gccagcgtct cttcgcgcac gccgatcatc tgcgctttgg cttcgttact
     5761 gtaggtgaca aatccgcgtt caaaccaggc ggagctaccg gcaatatcgg taatcacttt
     5821 cgctacccaa ccgccggtac aagactcagc agttgttacg gttgcgccac ggactttcag
     5881 cgcctgccca acctgttcgc ttaactgcat cagttcactg tcagtcatca caacctctgt
     5941 cagtcataaa tttatgccgg acaagatagc actttcgtca ggaagatggt gttggcgttg
     6001 caattttacg aggaggattc agaaaaaagc tgattagcca gagggaagct cacgcccccc
     6061 tctggcaaat agttactgca cgcgcgccag cgccacggct tgtcctaact gccagaccgc
     6121 cattgcgtaa tgggtgctgt ggttataacg ggtaatggtg tagaagttcg gcagaccgta
     6181 ccagtactgg taaccggtac cgacatccag acgcagcagg ctggcttgtt gatggttgcc
     6241 cagcggctgc tgtggcgtta aacctgccgc ggcaagctgc gaaatgctgt acctggtttt
     6301 gaagccattt ggcaagcctg gcgcctgacc gtttgccatt accgcgacct gatcgccttt
     6361 cacccagccg tgcgctttga aataattcgc cacgctaccg atcgcatcaa ccggatccca
     6421 caggttgata tgcccgtcgc cgctgaaatc taccgcatat tgtttgtaag acgacggcat
     6481 aaactgtccg tagcccatcg ccccggcaaa ggaacctttc agattgagcg gatcgtcctg
     6541 ctcgtcgcgc gccatcagca ggaaggtttc cagctcgcca gaaaaatact ccgcgcggcg
     6601 tgggtagtta aatgacagcg ttgccagcgc atcgaggatg cgagttttcc ccatcacgcg
     6661 cccccagcgg gtttcaacgc cgataatccc gacgataatt tccggcggta caccatacac
     6721 ctgccacgcg cgattcaacg catcttcata ctgattccag aaaaccacac cgttctgcac
     6781 gttgtccggc gtaataaatt ttttgcgata acgcagccat gctccattcg gacctgatgg
     6841 cggtttcacc gatgtggttg gtgcctggtt atccatcagc cgcagtaccg aatccagacg
     6901 tttcgcctgg gagagaattt cctgcaactg ctgacgatcg aaaccgtgtt tgttcaccat
     6961 tttgtcgatg aactgctggg cattcgggtt attggcgaag tcaccgccca tctgcatcac
     7021 attgtgctgc ggctcaagca ggaagccgcc agacggcgtt ccggtggtcg tttcagtctc
     7081 agtaggtttt ggcttgctgc tacaggcggc aagcaacaca aaaaggggaa gcaatgttac
     7141 ataacgacgc ttgaacatga ggggtccatt taacagattc aaccaggggc aagtatggta
     7201 aagcatcccg ccccgcacaa ggaagcggta gtcactgccc gatacggact ttacataact
     7261 caactcattc tcctcactac cttctttata caaactttca aaataaaata tttatctttc
     7321 attttgcgat caaaataaca cttttaaatc tttcaatctg attagattag attgccgttt
     7381 ggtaataaaa caataaatac tgaaggagag aacaatgata gaaaccatta ctcatggcgc
     7441 agagtggttc atcgggctgt tccagaaagg cggagaggtg tttaccggga tggtgaccgg
     7501 cattcttccg ctgttaatta gcctgctggt tatcatgaac gcattgatta attttatcgg
     7561 tcagcatcgt attgaacgat ttgctcaacg ttgcgccggt aaccctgttt cccgttatct
     7621 gctgctaccg tgcattggta cgtttgtctt ttgtaacccg atgaccttaa gtcttggtcg
     7681 ctttatgccg gaaaagtaca aacccagcta ttacgcggcg gcctcttata gctgccactc
     7741 aatgaatggc ctcttccccc atatcaaccc tggcgaactg tttgtttatc tcggcattgc
     7801 cagcggtctg acaacactga atctgccact tggcccactg gcggtgagct atctgctggt
     7861 tggtctggtc accaacttct tccgcggctg ggtaaccgat ctgaccaccg ccattttcga
     7921 gaaaaagatg ggcattcaac ttgaacaaaa agttcgtctg gcaggagcaa catcatgaca
     7981 cgcattcgga tcgaaaaagg aacgggtggc tggggcggcc cgcttgagct ggaagccacg
     8041 ccgggcaaaa aaatcgtcta tatcaccgcc ggtacccggc ctgcgattgt tgacaaactg
     8101 gcacagctta ctggctggca agctatcgac ggatttaaag aaggtgaacc cgcggaggcg
     8161 gaaattggtg tcgcggtaat cgactgtggc ggcacattac gctgcggcat ctatccgaaa
     8221 cggcgtattc ccaccattaa tatccactcg acgggcaagt ccggcccgct ggcgcagtac
     8281 attgtggaag atatttatgt ctctggcgta aaagaagaaa acatcactgt agtgggtgat
     8341 gcgacaccac aaccctcttc cgtgggccgt gactatgaca ccagcaagaa aatcaccgaa
     8401 caaagcgatg gtttactggc gaaggtggga atgggtatgg gttccgccgt tgccgtgttg
     8461 tttcaatctg gtcgtgacac catcgacact gtattaaaaa ccattctgcc gtttatggcg
     8521 ttcgtctcgg cgcttatcgg aatcattatg gcttctggcc ttggtgactg gattgcccac
     8581 ggtcttgctc cgctggcgag ccatccactg ggtctggtca tgctggcgct catctgctcc
     8641 ttcccgctgc tttcaccttt cctcggccca ggcgcagtta tcgcacaggt tatcggcgta
     8701 ttgattggtc tcggcaatat tccgccgcat ctggctttac ctgcactgtt tgccatcaac
     8761 gcgcaggcgg cctgcgactt catcccggtc ggtttgtcgc tggcggaagc tcgtcaggac
     8821 acggttcgcg tcggtgtccc ttctgttctg gtgagccgct ttttaaccgg cgcgccaact
     8881 gtactgatcg cctggtttgt ctccggtttt atctatcaat agaggctgaa acatgaccgt
     8941 tatttatcag accaccatca cccgtatcgg cgcgagtgcc actgacgccc tcagcgacca
     9001 gatgctcatc acctttcgtg aaggcgcgcc tgcggatctc gaagagtatt gcttcattca
     9061 ttgccacggc gagttgaaag gtgcactcca tcccggtttg caattttctc tcgggcggca
     9121 tcgctatccg gtgaccgccg ttggcagcgt ggcggaagac aaccttcgcg aactgggtca
     9181 tgtcaccctg cgcttcgatg gtttaagcga agcggaattt ccgggcactg tccatgtggc
     9241 aggccctgta cccgacgata tcgcgccggg atcggttttg aagtttgaat ctgttaagga
     9301 gtaaaaaatg aatcaggttg ccgttgtcat cggtggtggg caaaccttag gcgcgttcct
     9361 gtgccacggt ctggctgccg aggggtatcg cgtggcggtt gtcgatattc agagcgacaa
     9421 agccgcaaat gtggcacaag aaattaacgc cgaatatggt gaaggaacag cgtacggttt
     9481 tggtgctgac gccaccagcg agcaaagcgt tctggcgctt tctcgtgggg tagatgaaat
     9541 ctttggtcgc gtggatttgc tggtctacag cgccggaata gcaaaagcag cctttatcag
     9601 cgacttccag ctcggcgatt ttgaccgttc gctacaggtg aatctggtgg gttatttcct
     9661 gtgtgcccgt gaattttcgc gtttgatgat ccgcgacggg attcagggtc gcattattca
     9721 gatcaactcg aaatccggca aagtgggcag caaacacaac tctggctaca gcgcagcgaa
     9781 atttggtggc gtcgggctga ctcaatcact ggcgctggat ctggcggagt acggcattac
     9841 ggtgcattcg ctgatgctcg gtaacctgct gaaatcgcct atgttccagt cactgttgcc
     9901 acaatacgcg accaagctgg gtatcaaacc ggagcaagtc gagcagtatt acatcgataa
     9961 agtaccgctc aaacgcggct gcgattatca ggatgtgctg aatatgctgc tgttctacgc
    10021 cagtcctaag gcgtcgtact gcactggtca gtcgatcaat gtcaccggcg gtcaggtgat
    10081 gttctgatca acagcggaga tcttttaagg atctccgcgc gactaataga atgcctgatg
    10141 cgctacgctt atcaggccta caggacttcc gccactacat tacggagaag ttatggtatc
    10201 cgcactcatc accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggttggca
    10261 aatttctcgt tttaaccgtg ccttcgacac gctatgccag caagggaggg ttggcgtggg
    10321 ccgttccagc gggcgcttta aaccgcgggt cgtggtcgct attgcgctgg acgatcagca
    10381 gcgcatcgtc gacaccttgt ttatgaaagg actgaccgtc ttcgcccgac cgcaaaaaat
    10441 tcccgcaatt accggcatgc atgtgggtga tttacagccc gatgtgatct ttccccatga
    10501 tccactatca cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg
    10561 tgaatgttac ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat
    10621 gaaacctcgt cagcgtcagg ccgccattct ggagtatcta caaaagcagg gtaaatgctc
    10681 ggttgaagaa ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt
    10741 cattctggaa catgccggaa ccgtcattcg tacttatggc ggcgtggtgt tgaacaaaga
    10801 ggaatccgat ccgcctatcg atcataaaac gcttatcaac acccacaaaa aagagctgat
    10861 tgcagaagct gccgttggtt ttattcatga tggcgactca ataattcttg atgctggcag
    10921 taccgtgttg cagatggtgc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa
    10981 cagcctgcac atcgtcaatg cgctatccga actggataac gaacaaacta tactgatgcc
    11041 aggcggaaca tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt
    11101 cgagcatttc accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg
    11161 cgtaaccacc tttaacgagg tctataccgt cagtaaggcg atgtgtaatg ccgcgcgcga
    11221 agtgattttg atggctgact catcgaagtt tggtcgtaaa agccccaacg tggtttgtag
    11281 tcttgaaagc gtcgataagc tgattaccga cgcaggtatc gatccagcgt ttcgtcaggc
    11341 gctggaagaa aaagggatcg acgtgatcat aaccggagag agcaatgagt gaagcactac
    11401 tgaacgcggg acgtcagacg ttaatgctgg agttgcagga agcaagccat ttaccggaac
    11461 gtctgggcga tgatttcgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg
    11521 tggtttcggg aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acgcttgcca
    11581 gcaccggcac accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga
    11641 tgattgaaag ccgcgatgtg atgctgttta tctcttattc cggtggcgcg aaggaactgg
    11701 atctcattat tccgcgtctg gaagacaaat ctatcgctct gctggcaatg accggaaaac
    11761 cgacgtcacc gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag
    11821 cctgcccgat gcaccttgcg ccgacctcca gcaccgtgaa taccctgatg atgggcgacg
    11881 cgctggcgat ggcggtcatg caggcgcgcg gatttaatga agaagatttt gcccgctccc
    11941 acccagccgg ggcactgggc gctcgcttgc tgaacaaagt gcatcatctt atgcgtcgtg
    12001 acgatgccat cccgcaggtg gcgttaaccg ccagcgtgat ggacgcgatg ctggaactta
    12061 gccgcaccgg tttaggtctg gtggcagtgt gcgatgatca acgactggtg aaaggcgtct
    12121 ttaccgacgg tgatttacgt cgctggctgg ttggcggcgg cgcactcacc acgccagtca
    12181 atgaagcgat gacggtcggc ggcaccacgt tgcaatcgca aagccgtgcc atcgacgcca
    12241 aagaaatcct gatgaaacgc aaaatcaccg ccgcaccggt agtggatgaa aacggcaaac
    12301 tcaccggcgc aattaacctg caggatttct atcaggccgg gattatttaa tccttcagcc
    12361 ccagacgttt cgccagccta tgcaggttgg cgacgtcggt ttccagcatc cgcgcacagg
    12421 cggcccagtt gtgatgattt tgcgccagtg cctgacgaat ggtttcacgc tggaacgctt
    12481 ctgtcgcttc acgcaggttt tgcttaacaa cgggcaccgc cgccacttct ggcggcggca
    12541 acgtcacctc aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgctgcggg
    12601 ttgctctcgc cagcactacc gcccgatgaa tagcatgttc cagttcacga acgttccccg
    12661 gaaaattgta gtgttgcagt aaatttcgcg ctccggcact taataccact cgggagagcc
    12721 ccagccgcaa acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc
    12781 gctcacgcag cggcggcact gaaagtggaa acacgctcag gcggtggaac aagtcagcgc
    12841 gaaatcgccc tgccagtacc tcttcgcgta agtcacggtt agtcgccgcc agcacgcgca
    12901 catcgacccg caaactgcgg tcatcgccaa cgcgttgaat atcgccatac tgcaacaccc
    12961 tcagcagctt ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagcgtgc
    13021 cgttatccgc catttcaaac ttcccgctgc ggttactgat agcaccagta aacgcccctt
    13081 tcacatgccc gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat
    13141 agaccagcgg attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct
    13201 taccggttcc cgtctcaccg ctaattaaaa cgttgagatc ggatgccgcc acaatctcaa
    13261 tctctttttt caattgcgtc atgcctggcg acagaccgat catctgcgtc tgtttcaccg
    13321 cttcaaacgg cgcggcttcg cctggcagca tattctggct ttccagttgt tcaatcagca
    13381 atgcattgct taacgctccc gccgccagtg cggcaatcag ccgtagctct tcgtcgctga
    13441 aaacatcgaa ctgatcgggc tgcatcccgt cgagcgtcaa ggcaccgatc aggttttgcc
    13501 cggcaaacaa cggcaaacca acgcaggcgt gaaccttcag actctcctgc cctggaatca
    13561 aaccgtcata aggatcgggc aattcgctgt ctgcgggaaa gcgcaccacg tccccggcgc
    13621 gggcaatcgc ttccagccgt ggatgacctt ccagcgcaaa gcgtctaccg agtacatcct
    13681 tcgccagccc gtcgatggca agcggaataa actgccgcga gtcgtaacgt agcaacgcag
    13741 acgcatcgca ctccagcacc tgacgcagcg tggtgatcag gcgctgaaaa cgatcctgat
    13801 gaccaatccc acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca
    13861 tctttgcctc actgtcaatt tgactataga tattgtcata tcgaccattt tattgatagt
    13921 cattttgact actcattaat gagcacaatt ttatttatag agtaaaaaca attagataaa
    13981 aaactggcac gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga
    14041 ggttgctatg tctattgtgg tgaaaaataa cattcattgg gttggtcaac gtgactggga
    14101 agtgcgtgat tttcacggta cagaatataa aacgctgcgc ggcagcagct acaacagcta
    14161 cctcatccgc gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga
    14221 atttgtgcag aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtaattaa
    14281 ccatgctgaa gaggaccacg ccggggcgct gaccgaactg atggcacaaa ttcccgatac
    14341 gccgatctac tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga
    14401 gtggaatttt aatgtagtga aaactggcga cacgctggat atcggcaacg gcaaacaact
    14461 catttttgtc gaaacgccaa tgctgcactg gccggacagc atgatgactt acctgacagg
    14521 cgacgcggtg ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt
    14581 caacgatgaa gtggatcaga ctgagctttt cgagcagtgc cagcgttact acgccaatat
    14641 cctgacgccg ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt
    14701 gccagtcgat atgatagcga cctctcacgg cgtggtatgg cgcgataacc cgacgcaaat
    14761 tgtcgagctg tacctgaaat gggcggcgga ttatcaggaa gacagaatca ccattgtcta
    14821 cgacaccatg tcgaataaca cccgcatgat ggctgacgct atcgcccagg ggattgcgga
    14881 aaccgaccca cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat
    14941 cctgaccaat gtcttccgct caaaaggcgt actggtcggc acttctacga tgaataatgt
    15001 gatgatgccg aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa
    15061 acgcgccagc gctttcggct ctcacggctg gagcggcggt gcggtggatc gtctttccac
    15121 gcgcctgcag gatgcgggtt tcgaaatgtc gcttagcctg aaagccaaat ggcgaccaga
    15181 ccaggacgct ctggagttat gtcgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct
    15241 cgcgccgctg ccacagagca cggtgaatac ggtggttgaa gaagaaacct ctgccgccac
    15301 gacggctgac ctcggcccac ggatgcaatg cagcgtctgc cagtggattt acgatccggc
    15361 aaaaggcgag ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa
    15421 cttcctgtgc ccagaatgct ctctcggcaa agacgtcttt gacgaactgg catcggaggc
    15481 aaaatgagca acggcattgt gatcattggt tcgggcttcg ccgcccgcca actggtgaaa
    15541 aatattcgca aacaggacgc cagtattcca ttaaccctga ttgccgccga cagcatggat
    15601 gagtacaaca aacctgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt
    15661 acccgccaga cggcaggtga atttgccgag cagtttaatc tgcgcctgtt tccgcacacc
    15721 tgggtaacgg atatcgatgc cgaagcccat gtggtgaaaa gtcagaataa tcagtggcaa
    15781 tacgacaagt tagtgctggc aaccggtgcc agcgcctttg tcccgccagt gcccgggcgt
    15841 gagttaatgc tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg
    15901 gatgcccgac gcgtgttgat tgtcggcggt ggtttgattg gtagtgaact ggcgatggat
    15961 ttttgtcggg caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtcg
    16021 ttaatgccac cggaagtcag cagccgcttg cagcatcggt tgacggagat gggcgttcat
    16081 ctgctgttga aatctcagtt gcagggacta gaaaaaacag attctggcat tctggcaacg
    16141 ctggaccgcc agcgctgcat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg
    16201 gaaaccgccc tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt
    16261 tatctgcaaa ccagtaatgc cgatatttat gcgctgggcg attgcgcgga aattaacagt
    16321 caggtattgc cgttcctcca gccgattcaa cttagcgcga tggtgctggc aaaaaatctt
    16381 ctcggcaata acacgccgct gaaactcccg gcaatgctgg tgaaaatcaa aacgccggaa
    16441 ttaccgctgc atctggcagg cgaaacccag cgtcaggatt tacgctggca catttatacc
    16501 gaacgccagg gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg
    16561 gtcagtgagg atcggatgaa agaggcattt ggattgttga aaacgttgtc gatgtaggcg
    16621 agctacagtg ccccaaatgt cggatgcggc gctgacgcgt cttatccgac ctacagggaa
    16681 cgcatgtgtt gatcacataa tacgtttatg tcgcattgca ctgtcccaaa atgtcggatg
    16741 cgacgctggc gcgtcttatc cgacctgcgg ggacgcacgt gtaggccgga taaggcgttt
    16801 acgccgcatc cggcaatggt gtccaaatgc agcacgtttt atccgttctg gacttcccct
    16861 gccaaccaac gcgccgcaac aataaccccc tgccccagag acaacccgcc atcgcccgca
    16921 ggtaaactct gtgggaagag caacgtaaaa tcagcaagat aatgcgccag acgtgcacgc
    16981 agcaaacggt tatgaataac tccgccgcta aataccagcg tagtgatacc ccgcatcgtg
    17041 gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg catcatgaaa cgcccacgcg
    17101 cgttgattaa ccggagcctg ccagctcagc cactgctgcc agaaagtggc gagatccagt
    17161 tgattgtcca ccagcggcat cgtcaccgga tgcgtcactc tgtggcacgg agctgcgagt
    17221 gcctccagag cacaagccgc ttcaccttca taacttaacg tggctggcgc acagcccagc
    17281 gccgccgcta cggcatcgaa caaacgtcca cacgatgatg ccaacggcgc gttaattcca
    17341 cgctcaatag cccgcgccag cacgctccag ttttgctgtt gcacacttgc cgtttcagag
    17401 taattctgcc actccggcac aaagcgcagg cactgcgcca gcaggtttcg ccacggctgc
    17461 ttcgcagcca aatcgccccc cggaagcgcc actgcgggca agccgcccag gtgctggcat
    17521 tcgcgatagt tcacccgcaa gcactcgccg ccccacaaag cgcagttctc ccccatacca
    17581 ataccgtcga gcgtcaacgc aatgacatct ccgccatcca gaggccagag gtgttctgcc
    17641 agacacgccg ctgcgtgggc atgatgatgc agcaccgttt gcgtcggcag attcatctca
    17701 cgcgcccact ggctggagac atagcccgga tgcgcgtcat gcacaacgta ttgcggagtg
    17761 aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc actgcatctg gatgccatcg
    17821 tcacttaaat cgcccagatg ctgactcaac accgcttgtt cgccgcgcac caggcagaag
    17881 gtatttttca gatccgcgcc gagacacagc acaggcggaa catttttaaa gcccggaggc
    17941 aaagccagcg catccggcac atatccccgc gaacggcgca gcatttcgcc gctttcgcgc
    18001 accaccgaat catccatccg ctgcacgatg tcgcggttat gtatcaagaa tccgtcggca
    18061 atgccctgca aatccgccag cgcctgttcg ttgctgatag ccggtggttt accgctcagg
    18121 ttgccggagg tcatcaccag cgggcattgc agttcctgta acagcaaatg ctggagcggg
    18181 ttcgcaggca acattacccc gacttcgtta aggccagggg cgatatcatc acaaagctca
    18241 ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg gcgtggtaag caactgacgg
    18301 gcagcgtctg gtaaaccttc tgccgttggc aacatgaccg ccagctgttt cgccgggcga
    18361 tgtttgcgcg cccgaagtgt cgccaccgcg ttactgttac gtgcatcgca ggcaagatga
    18421 aatccgccaa tccctttgat ggcgacaatg ttgcccattt ttaactgtgc gatagctgcc
    18481 tgtaatgccg cctcttgttc tgcatgttca ccatgactta cccattcaag atgggggcca
    18541 cactccgggc aggccaccgg ctgggcgtgg aagcgacgat cgagcgggtc acagtactct
    18601 ttgtcacaag ccggacacag cggaaacgcc gccatcacgg taaacgggcg gtcgtaaggc
    18661 atggcgcgaa taatggtgaa acgcgggcca cagtgggtac agttgataaa cggataacgc
    18721 cagcgtcgtt cgcctggggt attcatttcg gcaaggcaag cagggcaagt cgcggcatcg
    18781 ggaacaattt gcgtattcat gacgccaccc gcgctctggc ggatggtgaa ctcagtgggc
    18841 agttgtgacc agataaacgg ctcacgctcg acgctatcaa tacgcgccag tggcgggcag
    18901 tgctgatgca attgaacaag aaacgtttcc ggatcttcca gcagccggac ttctacgcca
    18961 tcgccgtcat tacagacatc gccgtgaaga tttaattgct gtgccagttg ccagacaaac
    19021 ggacgaaaac cgacgccctg cactttgcca cgaatacgca gttggacacc gcaagatgtg
    19081 ttttttgcca ttgagttatt cccgccatca tgaattgcgt aacccgccca gccggacacg
    19141 acagcgtcgc atccggcagt cacaggtcgg cgatactgcc cgcttcgtat tctacgaata
    19201 tttccgggaa ttcctttgat accagaacag ttctgtaaga tttttagaac atcagcgccg
    19261 tgcggcggcg tttttctgcg ctcagttgtt caagtttatt acgatcgaca caaatcagcg
    19321 catgggtcgg gcaagccgcc atacacgccg ggccgtcttc acgatggttg cacaggtcgc
    19381 atttattggc ttcggctttg tcagcccgta cattcagccc tgcgccgctg ttgcgaatca
    19441 ccggacgcac caccacttcc atcgcaccat acgggcaagc cacaacgcag gttttgcaac
    19501 caatacaacg ttcctgcatg acatgaacaa accctttatc acggctgata gcaccattcg
    19561 ggcagacgtt agcgcacggt gcatcttcac actgacggca aactgtcgcc gtggaaatgt
    19621 tcacaccttt aatgacatgg atacgcggta aaaatgtttc cggggtcagc gatgcacagt
    19681 cctgattttc ctgatgagaa accacgcacg ccacttcaca ggtacggcaa ccaatacatt
    19741 tactcgcgtc agcaatgatg aaacggttca tcaaattctc cagcaataac agttaatgcg
    19801 ccgatacatt cacaaatcat gccagttttt aatttactgt tatttaaaga aattaaattc
    19861 tgtaatgcag gaaaaacgat gtcatcgaca ctagtgacga tgacatgtga tgacagtatt
    19921 tatcgttgcc agtccacatc tgggtacttt tttccgacaa gtcgttctgg tggtataaaa
    19981 aagtaattgc cagtaacggg gaggatttaa gggaagcaac cgtgccggat acggcgtgaa
    20041 caatgaccac tttgtcagct atccatatga ttgtattttg aggttcatcg cagattagcg
    20101 atgaaccttt attaaataat acaattccga ctgaaataac aggtaaggat acaaaatggg
    20161 ttcatgggga atggaggctc tcgaaagcga cgaagggctg gatttgatta attgggtaga
    20221 ggaacaactg caagatgatt cgacctttga tgccgagtca attgttcaac gcttaagcca
    20281 gcatgaagat ttgttcggat ttcagggaga tgaagaattt ctctacgata ataatgttat
    20341 cggtctcgtc gaacttatca ttcaaaaagc agccggggaa aaaatcacat cgagtaaaca
    20401 aattgatcaa ctcgacagtt atcggctcac gtcgattttc agcaaaaaac tccagggccg
    20461 tttacaaaca atcgacgaca cacatgagtg gataatgctt tttgaggggc gagcacgcga
    20521 aaaggcaaaa gcatatctga ttgagattac tgataaatta agagtagtaa agacaacagc
    20581 ctgaatgaca aacaagggcg cgcaaaatta agaatctgcg ccctttcaaa gcccgacagg
    20641 cgtagcgcat caggcgattt agcgtctgcc atcagtcaaa gaaaccattc cggcttaacg
    20701 ccaaatcggg ctgaaagtgc cttaatgtgg gatatcgtca aggatcgttg tccagacaag
    20761 atctggctga ccagggattt agagccgatt tcgttcttca aatccgcata ggtcagatta
    20821 tgttgatcaa tcagcgtgcg aagtaacgcc gcaccaacag gcattgccgc aacggctttg
    20881 tcgaactctg caaacttttc gttgttgttt tcatattctg caatgcggct cgtcagaaag
    20941 tcgataagtg gattggtgtc atcttcttca atcaaatatt cgaccagttc cagtgcttca
    21001 cgataatcat cttctgagtc actgcctccc agaaagggga ctgcattgac cagttctctt
    21061 gttgctttaa cagcgcgtac agcattagca gtcattcttt gttctctctg tagtaacgtg
    21121 tcagtttgtc atactcggcg tgattagtta tgtgcttcac aaagaaccgc ttattaacaa
    21181 agtttatgta ggcaatcacc cttaggttgt tcccacctac atccagaacg taccatttgt
    21241 tgcgatattt aaaattgtcg aggttgggaa acgccgttcg catttcttct ggcgtagaaa
    21301 aatccgtttc cttaataacc cgatacaatg cctgaagagc cagagcgtcg ttaggatatt
    21361 tacgcgcaga ctcttcaaat ggcgctttag aaatgatgtg catttttaag ttttaacttc
    21421 cttatagtaa gtaaacagcc acatctgcga gttctatcct tatatgttcg gaacactata
    21481 gcttatcaaa tccaagttca caatatgtaa acacttgcgc acgatataat tactacaaaa
    21541 atcctcatag ccactctgca aaaagtcagt tctgattcac cccatcaaac acatcaccct
    21601 ttattaaaca acgctgtgtc agcgccagat ctttcgtttg cgggttacgt ccggcgacag
    21661 gtttaatgac gagtttacgg ttggaggtat aggtaaaacg cgtgagtgcg gggtagctta
    21721 acttgccaaa ccagccgtga acgcgctgcc atttggcctc ttttacctga tacaaactgg
    21781 ttatccagta accggaatca aactgcatat ccagcaattg cgtgtgacca ttgccttgta
    21841 aatcaagcaa atcatcaacg ccagtttcgt ttgcggtgta aaaaccatcg acctggaaaa
    21901 cacacgggcg accttgctga ttaaaggtca tcaggatcaa aaacgaggaa ggcgccagcc
    21961 cgtttcccga aacacctcgc cagataacca aatcctgaat gccattacga tcgaggtcag
    22021 cacggtaaaa ttgcgcatta cccgccatat tttgcagcac acccacaggc acaaaccagg
    22081 atttaccggc acggtcctta gcggttaatc gccagtaatc atcttcattt ggcatcttct
    22141 caagcgttac gcctgcaacg cccacttttc taaaatgcca gttttgatcg gcatcataag
    22201 gcatggtcag gagaggtttc agcgcccgct tttgggcgta ttcgctgtca ctcattggca
    22261 gatattcggt aaagctggtg gcctgtgcag gcagcactaa gctcaataaa aaagcgcaca
    22321 tccctgtcct gatgttcaat tccctgaatt ccttactgaa aaactactct tcttccgccg
    22381 ctaactgcgc gaagccgcca tttccttccc agccttctag ccgctgatac accgtttcca
    22441 ccgcatcttt aatctgctga gtcatcgggt agtaaaagcc gacgatatcc ggctggatgc
    22501 caaggaaaat cacttcgcca atatcttctt tcaactggtc gataagatag ttcagcggca
    22561 tgttatgggt agtcatcata aacatctcgg cgatgtcagc cgggtcgatg atgcggatct
    22621 cgccagggtt tagccccata tccgtggcgt cgacaataag cagccgtgtc gggcgcagtt
    22681 cgcgaatagc gacaatgtcg ttttccggtg cgctaccgcc gtcaatcact acccagttac
    22741 ctttcggcgc ggcagcgcac ttttccgcca gtagcgggcc tgcgccatca tcgcccatca
    22801 tgctattgcc aacacagagt aaaacgtcag tcacgcagtc tcctcaccat caaatagatg
    22861 gcgttttcct gatgaatatc atgcagcata cttaacagca ttgtactcca ttccttctgc
    22921 tgcgaagttt gcgctgcccg ggctttatcg aatgcgttgg ccagcatcgg cacatggtta
    22981 atgtcgatga cgatctcacc atatttcggc acgccttcca ttttacggcg ggcttcgctg
    23041 cccgcctcca gagtggcaat ccatgccaga tattcatccc acgggcaggt aagcgccgct
    23101 tccaggcaat cgataacccc aaggtggtga ccaatcgcca ggctgtaata gaccacctgc
    23161 tgcgcctcgg cgggcgtggc atcgttctca tcaatgaatt tacggctcag ttgactgaac
    23221 accacctttt cactcatcgg atacgcgcct cttcaacaac atgattcaga tggctgacaa
    23281 tctcgttcag acgcggatcg ttttccgctt ccagccagcg tgcaacctgt tcttcgccct
    23341 gccctaactg tgtcaggaag tcatcggcaa tctgacgacc gtaacgataa cccgccagtc
    23401 ggcgtgcttc gcgatccact ttcacgcgca gcggctgcac catatcgcca tgcagaatct
    23461 ccgccggttg ttcatcctgc tcacccggcc cacgggcgtg aattttctgc tccagcaggc
    23521 cgagcgccat cgcaaagccg tacagcgtgg cggcaggcgt tggcgggcac cccggaatgt
    23581 agacatccac cgggacgatt ttatccgtac cgccccacac gcagtagaga tcgtggaaga
    23641 tcccgccgct gttaccgcag gcaccgtagg agatacagat tttcgggtcc ggcgcggact
    23701 gccacgcacg cagcgcaggg gatcgcattg cacgggtgac cgcgccggta aacagtaaaa
    23761 tatccgcatg acgcggtgac ggaacaactt taatgccgaa gcgttctgca tcgaacagcg
    23821 gcgaaagcgt ggcgaaaatt tcgatttcgc aaccgttaca gccgccgcag tccacgcgat
    23881 aaacgtaggc agaacgtttg atttttttca gtaacgacgc cttcatgctg tcgatggatt
    23941 catccaccgt catcgggacc ggaatgccgt tggcgtcacg ggggcctaat aaattgctca
    24001 tcagatggcc tctttcatat ggcgagtcag ttcaatacgg tcggacggca ccaggcattt
    24061 ctggcgctta cattccgggc aagtctcaaa gctttcgcgg tggttttccg cgcggctgtc
    24121 gccgttgtgc ttaagcagcg caatggcgta gtcgatctct ttctggacgg cgaaaggacg
    24181 attgcagacg cggcagttgc acagcgcgaa gcgggactgt tgcaggaagt cttctttctt
    24241 ccacaccgcc agttcgtatt cttgcgacag tttgatcgcc gctgtcgggc agacttcttc
    24301 gcagcgccca cagaagatgc agcgcccaag gtcaaactgc cacgccagct caccagtggc
    24361 gaggtcggtt tcaaccgtta aggcgtttga cgggcaggca ttgacgcaag ccgcacagcc
    24421 aatgcactgc tgcgggttct gctctggctt accacggaag tttttatcaa ccgcaatcgg
    24481 ctccagcgga taagacgaag tcgccgtgcc ggttttgatg acttttttga taaaggtaaa
    24541 catggcgatt ccttatttca gcggcgagtt tttacgctca atgctgtaac gctcgagttc
    24601 tttgtacggc accactttgc tcttcttctt acgtacatcg accacggtca tgcggtcggt
    24661 acaggagtag caagggtcca ggctaccgat aatcaacggc gcatcggaaa cggtgttacc
    24721 gcgcagcatg tagcgcaggg tcggccagtt cgcgtaggtc gccgcacggc agcgccagcg
    24781 gtacagcttc tggttgtcgc cggtcatgct ccagtggata tcgtcgccgc gcggcgcttc
    24841 ggcaaagccc agcgcgaaac ggtgcggaat gtaggtaaag ccttccacca tcagcgggcc
    24901 gcccggcaag ttatccagac cgtaatcgat catattcagc gcggtataga cttcgttgat
    24961 acgcactttc agacgggaaa taacgtcgca gccctgctcg ctgtggactt ccattggcag
    25021 caggccataa ccaacaaacg ggtgatcggc acgggtatca cgggcgtgac cgctggcacg
    25081 aaccatcggg ccaacgttac tgaagtcgcg agcgatttcc gggtccagac gaccaatgcc
    25141 gacggtgcgt tgttccatgt tcggtgtgct cagcagcaca tccaccagct cctgcacttc
    25201 acgacgcatc tgttgtgcca gctggcgggt ctggatcatg tcgtctttca gcagatcgcg
    25261 acgaatcccg ccgatcaggt tcaggccgta ggttttacgt gcaccggtaa ggatctctgc
    25321 cattttcatg gaggtttcac gcacgcggaa gaactgcata aagccggagt cgaagccggt
    25381 aaagtgacag gccaggccga gattgagcag atgcgagtgc aggcgttcta cctccagcag
    25441 aatggcgcgg atcatctgcg cgcgttctgg cacctgaata cccatcgcgt tttccaccga
    25501 cgtggtatag gcggtgctgt gagcaaagcc gcagatcccg cacacacggt cagagaggaa
    25561 ggtcacttcg ttgtaaccca tacgggtttc cgccagtttt tccataccgc gatggacgta
    25621 gaacaggcgg tagtcggcgt cgataatgtt ttcaccatcg acgaacagac ggaagtgacc
    25681 cggttcgtca gaagtgacgt gcagtggacc aatcggcacg acgttgtttt tcttgtcgcc
    25741 cagttcgttg atgaactcgt aggtttcagc atcggtagtc ggtgccggac gctgacgata
    25801 atccatgctg tctttacgca gaggataaag ttcatccggc cagtcatccg gcagcaccag
    25861 acggcgttca tccggcagac caaccggaat caaaccgtac atatcgcgca cttcacgctc
    25921 gccccacact gccgccggaa cgcgcggcgt cacggaagga tactccggtt tgttggcgtc
    25981 aacttcgacg cgaaccgtta cccaacactt ggtgcccttc tccatcgaca gcacgtaata
    26041 aacggcgtaa tgaccattca gtttgcgttc gtcgttacca aacaacaccg acagccagcc
    26101 accctgcttg tagtagagaa actccaccac ttccggcagg tagttcacct ttacggtcac
    26161 ggtcagctga tctttggtct gccaggcgtg gtccagcacg acgccaggaa atgcctcatt
    26221 cagcgcggcg agataatgtt gacctaattt ttcttcagac atgctcaaac tctctttaat
    26281 cacgccgcca gcaaggagac gaacgctaaa aatgcaaagc caaacccggc ccaggtaatg
    26341 cgcggagtaa tatcaagacg cagacgggcc atgctgtttt cgaacagcgc gataaccagg
    26401 acgccaacca ccagtttgac gatggcaatc accagcgcca gcagcagccc accgacggtg
    26461 aaggtttcca tttgtcccca cggaataaac accccgacga acatctgcaa caccaccagc
    26521 tgtttcaggc tgatgcccca tttcatgacg ccaaagccgc tgccgctata ctcagagagc
    26581 ggaccttcct gcaactcctg ctcggcttcc gccaggtcga acggcagttt gcccatttcg
    26641 ataaaagtgg cgaacgcaca ggcacaaagc gccagcacca gcgggatgct ctggctcagt
    26701 ggccagtgat aaacggtgtc ggtgatgttg ctgatgttgg tggaaccggc aacctgtgcg
    26761 gcgacccaca gaccaagcag cagcatcggt tcaaccaaca cgccaagcat cgcttcacgg
    26821 ctcgcgccga tagcggtaaa cgggctacca gtatccagac cagaaatggc aaagaagaaa
    26881 cgcgcgatgg cgaagagata cagtaaggtg atcaaatcgc ccagttgcgg cagcggagag
    26941 ccgacggtca ccaccggcag cgcagtggcg atagtcagca tgacgcccac catcacatac
    27001 ggcgtcaggc ggaacaccca gccggaggca tccgggccaa cgctctggcg gcccagcagt
    27061 ttgataatgt cgcgatactc ctgcaacaca cccggcccac gacggttatg cagacgggcg
    27121 cgcgctacgc gggtaatacc ggagagcagc ggcgcaacgg caaatagcac cagcgcctga
    27181 attaacggat ataaaacact cattctcagg ctcctcgtga aacaataatc accaccagca
    27241 ccgccagttc aaccagcgcc atccggcgga acagcaacgc actcccctcg cactgccagc
    27301 ccggcaccag agacaccgga ttcagccatt tgcgtagttt cagcaccggc gcaaacgcct
    27361 gtttcaccgg catggcaaaa ccgtgagcgg taatcaccat tgatttttca tgatcgtagc
    27421 cgcacaccca ggccgcaccg cgggaacgcg acggcaaacg atcgcctttg caaatcgcca
    27481 taatgatgaa tggcagcagc gggcagtcaa tcagcagcaa cgtgatcatt ggttgagaaa
    27541 cggtggtgtt agcaggctcc agcggcagag gtacagcagc agagagcatc ggcagtagcc
    27601 acggcgcagc aacaccgcca attacgcagc aaatcgccag tgccactacg cttacgctca
    27661 tcaggagcgg cgcacaggtg gcgttttcgg cctctttggt acgcggcgcg ccgaggaaag
    27721 taacgccata aactttcgcc atacacatca ccgccagcgc accggtaatt gccagcccca
    27781 cggcgagcag cggcccgaga agacgggcaa caaacgcgcc actattgctc agtttgaaga
    27841 aggattgata gataacccat tccccggcaa aaccgttcag cggcggcagc gcagccattg
    27901 ccatcagccc gactaacatg gcgatggaga taaccggcat tttcttgcca ataccaccga
    27961 gtttttcgat atcgcgatga ccggtacgga accagacgct ccccgcacca aggaacagta
    28021 cgctcttgaa caggctatgg ttaatcagat ggtacagacc accaaccaga ccaagggcaa
    28081 tcagcgccgg ttgttcgagc gcgatacccg ttacgccagc gcccagcccc agcaggataa
    28141 tgccgatatt ctccagggtg tggtaagcca gcagacgttg gatattgtgt tccatcaacg
    28201 catacagacc gccgacgaat gcggtgatca taccgagcac cagcagcgcg atcccccacc
    28261 acagcggtgc gttaccgccc agcagtgaca gggttaaaat gcccagcagg ccaattttca
    28321 tgactaccgt agaaaacaac gcggcagctg gcgcagaggc gttagcatgt gcctgtggca
    28381 cccagccgtg cagcggaata atcccggcca gcaggccaaa gccaatcaca ccgagcagcc
    28441 agatatcgga accgagcggc agctgttgca tacgcatatc cagcaggcgc agatccagcg
    28501 tgccgtaacg ctgccacagc agccagcagg caatcgccag cagcagagtg ccaagacgcc
    28561 ccagcgcaaa ccacagtttg ccctctttgc tgttgctggt gaggaacacc gcacacaggg
    28621 ccatgatttc ggccattacc acgaacatgc cgaggttgct ggcgatgacg gcacagacgg
    28681 cggcagccat caacatattg atctgcaagc cgttgcattt cacctgcgcg tggcgatgcc
    28741 agtcaatgtt gtagaggctg acaaacagac cgcacagacc gagcgtaatc agccaaatcg
    28801 cgttaagcgg agagatttgc acatcgtagc ttaccagcga caacgcaccg ctcacgccaa
    28861 ccgcgccagt cagcacagta aagcccgcgg ctgccgtata caggctaccc accgcgccgc
    28921 caattccggc tatccagccg cttagcgcct tttgaaaaga aaagagaaat gccagaacag
    28981 cggcggcgac aaaccacgcc acgccgctat tgatcaggga aattgcgctc atttagcctc
    29041 tccactttga gcctgctgaa acaaggtgag atcgccaaag tccgtgttaa aagtcagctc
    29101 acgcttacgt ttgctgacgc gggcgatatc ggtgttatcc accagatgca gggctttagt
    29161 cgggcacatc cgcacgcagg ccggaccttg ttcatcaaag ctacaaaggt cacatttgac
    29221 ggcgatcgcg cgaatacctg gcacccagtc aagcaatgtg ctgacacgcg ccggagcagg
    29281 cggtgccggt ggcgctttcg gggtattggc gtttgccgga atatccagcg gacggctgcc
    29341 ggaaaattca attgcgccaa acgggcaggc gataccacac agtttgcagc ttacgcacag
    29401 actttcattc aactgcacgg ccccatcaac gcgggtgatg gcgttaaccg ggcagaccgt
    29461 tgcgcagggt gcatcttcac agtggtgaca gagctgcggc gcagattctt tttcattgag
    29521 catcactctc aggcgcggca ttgattgcag gccgtgctgg cgatgcgtct ctgaacaggc
    29581 agcctcacaa gtgtggcagc cgatacagag cgtggagtca gcaattacaa aacgattcac
    29641 caggcattcc tcaggtgatt gtcatttttg acgaaaacat gccgatgaaa tgtcattttc
    29701 gacactcatc gacacgccca tccccaaaca ggcgtaacgc ctgcaaaacg ggcaaagcct
    29761 cagctcatgc tgccgggctt tgtcccttta ccagttggct taaattcacc ggcacattgt
    29821 tttcaatggc ggtatataag ctgtcgtaaa cgccagcgat tttttcgaga tagtgttcca
    29881 gaacctgttc gcgatcgcgg atgttgacct tgccatcaat catgccgtca atgctcagtt
    29941 gcgcctgcgc aatccgctgt ttgtcttcgc catcttttgt ttcgacgtaa tactcgatgg
    30001 tgtggctgtt aaagccatcc gccagggtga cgtaaatgcg aaaatgttca aaaaggacga
    30061 aacagagttc tttgtccggc gcgcagctca tggcgtgatg caggcgcgct ttcgataacg
    30121 tgatcccctg aaccagcgaa ttgcagtaga tgtgccactg gtcctgtagg cgacgatgcc
    30181 gctgtgcgat gtaatcggct ttctcgctta tttcccaaat agtcattgtc aggttacccg
    30241 tttaacagag atgccagctt taagcatttt ctgtgccaac ttttaattca ttgttattaa
    30301 atcgatttct aagctaaaga tgaatttcgt cgccgtgtcg acgtgtcatt tcgacatcat
    30361 cgacattatt caccgcaggg ataatcaaca ctggcacaat tattgcttat agctggcaat
    30421 agttaatggg aggcgatatg cacgaaataa ccctctgcca acgggcactg gaattgatcg
    30481 aacagcaggc cgcaaaacac ggcgcaaaac gcgtaactgg ggtctggctc aaaattggcg
    30541 cattttcttg tgtcgaaacc agctctcttg ccttttgttt tgatctggtt tgccgcggca
    30601 gcgtggcgga aggttgtaaa ctgcacctcg aagaacaaga ggccgaatgc tggtgtgaaa
    30661 catgccaaca gtatgtgacg ctactgaccc agcgcgtccg ccgctgtcca cagtgtcatg
    30721 gtgacatgct acagattgtg gcagacgacg gtttacagat tcggcggata gaaatagacc
    30781 aggagtgagc gatgtgtaca acatgcggtt gcggtgaagg caacctctat atcgagggtg
    30841 atgaacataa ccctcattcc gcgtttcgta gcgcgccatt tgccccggcg gcacgcccga
    30901 agatgaaaat caccggcatt aaagcgcctg aatttacccc cagccagact gaagaaggcg
    30961 acctgcatta cggtcatggc gaagcgggca ctcacgcgcc gggcatgagc cagcgtcgga
    31021 tgctggaagt cgaaattgac gtgctggaca aaaataaccg tctggctgaa cgcaaccgcg
    31081 cacgctttgc tgcccgcaag caactggtgc tcaacctggt ttccagccct ggttccggta
    31141 aaaccaccct gctgacggaa actttaatgc gcctgaaaga cagcgttccg tgcgcagtta
    31201 ttgaaggcga ccagcaaacc gtgaacgatg ccgcgcgcat tcgtgctacc ggcacgccag
    31261 cgattcaggt gaacaccggt aaaggctgcc atcttgacgc acagatgatt gccgacgccg
    31321 cgccgcgcct gccactggac gataacggta ttctgtttat cgaaaacgtc ggcaacctcg
    31381 tgtgcccggc cagcttcgat ctcggtgaaa aacacaaagt ggcggtgctt tccgttaccg
    31441 aaggtgaaga caaaccgctg aaatatccgc atatgtttgc cgccgcctcg ctgatgctgc
    31501 tcaacaaagt tgacctgctg ccgtatctca actttgacgt tgagaagtgc atcgcctgcg
    31561 cccgtgaagt caatccagaa attgaaatca tccttatttc cgccaccagc ggcgaaggga
    31621 tggaccagtg gctgaactgg ctggagacac agcgatgtgc ataggcgttc ccggccagat
    31681 ccgcactatt gacggcaacc aggcgaaagt cgacgtctgc ggcattcagc gcgatgtcga
    31741 tttaacgtta gtcggcagct gcgatgaaaa cggtcagcca cgcgtgggcc agtgggtact
    31801 ggttcacgtt ggctttgcca tgagcgtaat taatgaagcc gaagcacgcg acactctcga
    31861 cgccttacaa aacatgtttg acgttgagcc agatgtcggc gcgctgttgt atggcgagga
    31921 aaaataatgc gttttgttga tgaatatcgc gcgccggaac aggtaatgca gttaattgag
    31981 catctgcgcg aacgtgcttc acatctctct tacaccgccg aacgccctct gcggattatg
    32041 gaagtgtgtg gcggtcatac ccacgccatt tttaaattcg gcctcgacca gttgctaccg
    32101 gaaaacgttg agtttatcca cggtccgggt tgcccggtgt gcgtactgcc aatgggtaga
    32161 atcgacacct gcgtggagat tgccagccat ccggaagtca tcttctgtac ctttggcgat
    32221 gccatgcgcg taccagggaa acaggggtcg ctgttgcaag caaaagcacg cggtgccgat
    32281 gtgcgcatcg tttactcgcc aatggatgcg ttgaaactgg cgcaggagaa tccaacccgt
    32341 aaagtggtat tcttcggctt aggttttgaa accaccatgc cgaccaccgc catcactctg
    32401 caacaggcga aagcccgcga tgtgcagaat ttttacttct tctgccagca cattacgctt
    32461 atcccgacgc tgcgcagttt gctggaacag ccagataacg gtatcgacgc gttcctcgcg
    32521 ccgggccacg tcagtatggt tatcggcact gatgcctata attttatcgc cagcgatttt
    32581 catcgtccgc tggtggtcgc tggtttcgaa ccccttgatc tacttcaagg cgtggtgatg
    32641 ctggtggagc agaaaatagc ggcccacagc aaggtagaga atcagtatcg tcgggtggtg
    32701 ccggatgccg gtaacctgct ggcgcaacag gcgattgccg atgtgttctg tgtcaacggc
    32761 gacagcgaat ggcgcggctt aggcgtgatt gaatcttctg gcgtgcacct gacgccggat
    32821 tatcaacgat tcgatgccga agcgcatttc cgcccggcac cgcagcaggt ctgcgatgac
    32881 ccgcgtgcgc gttgtggcga agtcttgacg ggcaaatgta agccgcatca atgcccgctg
    32941 tttggtaaca cctgtaatcc tcaaaccgcg tttggtgcgc tgatggtttc ctccgaagga
    33001 gcgtgcgccg cgtggtatca gtatcgtcag caggagagtg aagcgtgaat aatatccaac
    33061 tcgcccacgg tagcggcggc caggcgatgc agcaattaat caacagcctg tttatggaag
    33121 cctttgccaa cccgtggctg gcagagcagg aagatcaggc ccgtcttgat ttggcacagc
    33181 tggtagcgga aggcgaccgt ctggcgttct caaccgacag ttacgtcatt gacccgctgt
    33241 tctttcctgg cggtaatatc ggcaagctgg cgatttgcgg caccgccaat gacgttgcgg
    33301 tcagtggcgc aattccgcgc tatctctcct gtggctttat cctcgaagaa ggattgccga
    33361 tggagacact gaaagccgta gtgaccagca tggcagaaac cgcccgcgcg gcaggcattg
    33421 ccatcgttac tggcgatacc aaagtggtac agcgcggcgc ggcagataaa ctgtttatca
    33481 acaccgctgg catgggcgca attccggcga atattcacag gggcgcacag acgctaaccg
    33541 caggcgatgt attgctggtt agcggtacac tcggcgacca cggggcgact atccttaacc
    33601 tgcgtgagca actagggctg gatggcgaac tggtcagcga ctgcgcggtg ctgacgccgc
    33661 ttattcagac gctgcgtgac attcccggcg tgaaagcgct gcgtgatgcc acccgtggtg
    33721 gtgtaaacgc ggtggttcat gagttcgcgg cagcctgcgg ttgtggtatt gaactttcag
    33781 aagcggcact gccggttaaa cctgctgtgc gcggcgtttg cgaactgctg ggactggacg
    33841 cccttaactt tgccaacgaa ggcaaactgg tgatcgccgt tgaacgcaac gcagcagagc
    33901 aagtgctggc ggcgttacat tcccatccat tgggaaaaga tgcggcgctg attggcgaag
    33961 tagtagaacg taaaggtgtt cgtcttgccg gtctgtatgg cgtgaaacga accctcgatc
    34021 taccgcacgc cgaaccgctt ccgcgtatat gctaataaaa ttctaaatct cctatagtta
    34081 gtcaatgacc ttttgcaccg ctttgcggtg ctttcctgga agaacaaaat gtcatataca
    34141 ccgatgagtg atctcggaca gcaagggttg ttcgacatca ctcggacact attgcagcag
    34201 cccgatctgg cctcgctgtg tgaggctctt tcgcaactgg taaagcgttc tgcgctcgcc
    34261 gacaacgcgg cgattgtgtt gtggcaagcg cagactcaac gtgcgtctta ttacgcatcg
    34321 cgtgaaaaag acacccccat taaatatgaa gacgaaactg ttctggcaca cggtccggta
    34381 cgcagcattt tgtcgcgccc tgatacgctg cattgcagtt acgaagaatt ttgtgaaacc
    34441 tggccgcagc tggccacagg tgggctatac cccaaatttg gtcactattg cctgatgcca
    34501 ctggcggcgg aagggcatat ttttggtggc tgtgaattta ttcgttatga cgatcgcccc
    34561 tggagcgaaa aagagttcaa tcgtctgcaa acatttacgc agatcgtttc tgtcgtcacc
    34621 gaacaaatcc agagccgcgt cgttaacaat gtcgactatg agttgttatg ccgggaacgc
    34681 gataacttcc gcatcctggt cgccatcact aacgcggtgc tttcccgcct ggatatggac
    34741 gaactggtca gcgaagtcgc caaagaaatc cattactatt tcgacattga cgatatcagc
    34801 atcgtcttac gcagccaccg taagaacaaa ctcaacatct actccactca ctatcttgat
    34861 aaacagcatc ccgctcacga acagagcgaa gtcgatgaag ccggaaccct caccgaacgc
    34921 gtcttcaaaa gtaaagagat gctgttgatt aatctccacg agcgggacga tttagccccc
    34981 tatgaacgca tgttgttcaa cacctggggc aatcagattc aaaccttgtg cctgttaccg
    35041 ctgatgtctg gcgacaccat gctgggcgta ctgaaactgg cgcaatgcga agagaaagtc
    35101 tttaccacta ccaatctgaa tttactgcgc cagattgccg aacgtgtggc aatcgctgtc
    35161 gataacgccc tcgcctatca ggaaattcat cgtctgaaag aacggctggt tgatgaaaac
    35221 ctcgccctga ccgagcagct caacaatgtt gatagtgaat ttggcgagat tattggccgc
    35281 agcgaagcca tgtacagcgt acttaaacaa gttgaaatgg tggcgcaaag tgacagtacc
    35341 gttctgatcc tcggtgaaac tggtacaggt aaagagctga ttgcccgcgc gatccataat
    35401 ctcagtgggc gtaataatcg ccgcatggtc aaaatgaact gcgcggcgat gcctgccgga
    35461 ttgctggaaa gcgatctatt tggtcatgag cgtggggctt ttaccggtgc cagcgctcag
    35521 cgcattggtc gttttgaact ggcggataaa agctcactat tcctcgatga agtgggcgat
    35581 atgccgctgg agttacagcc gaagttgctg cgtgtattac aggaacagga gtttgaacgt
    35641 ctcggcagca acaaaatcat tcagacggac gtacgtttaa ttgccgcgac taaccgcgat
    35701 ctgaaaaaaa tggtcgccga ccgagagttc cgtagcgatc tctattaccg tctgaacgta
    35761 ttcccgatcc acctgccgcc actacgcgag cgtccggaag atattccgct gctggcgaaa
    35821 gcctttacct tcaaaattgc ccgtcgtctg gggcgcaata tcgacagcat tcctgccgag
    35881 acgctgcgca tcctgagcaa catggaatgg ccgggcaacg tgcgtgagct ggaaaacgtc
    35941 attgagcgcg cggtattgct aacacgcggc aacgtgctgc agctgtcatt gccagatatt
    36001 gctttaccag aacctgaaac gccgcctgcc gcaacggttg tcgcccagga gggcgaagat
    36061 gaatatcagt tgattgttcg cgtgctgaaa gaaacaaacg gcgtggttgc cgggcctaaa
    36121 ggcgctgcgc aacgtctggg gctgaaacgc acgactctgc tgtcacggat gaagcgactg
    36181 ggaattgata aatcggcatt gatttaacag atgtggttat actcgatcaa cattcaatga
    36241 aaggaggcag tatggcagtt tcagcaagaa atcaattaac cggaacagtt agcgcagtag
    36301 caatgggcgc tgtgaatgat gaagtagaac tgacgctggc aggtggtgca aagctggtgg
    36361 caattgttac acacagcagc cagcaagcgc tgggtctggc gaaagggaaa gaagcgattg
    36421 ccctgattaa agccccgtgg gtcacgctgg caacggaaga ctgtggactg aaattctctg
    36481 ctcgcaacca gtttgctggc agcgtgtcga caatcaccga aggcgcagtg aacgccactg
    36541 tccatatcaa aacggatgcg ggttttgaaa ttgtggcagt ggtaacaaat gaaagtcagg
    36601 atgaaatgaa actcaccact ggctcgcgcg ttattgcatt gatcaaagca tcagccatcc
    36661 tgattgccac caaagcgtaa acgccttatt tgccggagag tatggctctc cggcagattt
    36721 atttctttgg acgatatttt tctggcaact ccggcaccgg acgcttgtca tcgatgagat
    36781 gacgcacggt taagatcgga tgacgccaca gcattctcgg cccggcccaa cgcataatct
    36841 gtttcatctc ttcacgcttt gcaggctggt aacagtgcac cggacactgc ttacaggctg
    36901 gtttctcttc accgaacaca catttatcca gccgcttttg cgcgtaagca aacaacgcct
    36961 cgtaatgctc cggctctgct gacgcctgcg ggcatttcgc ttgataaaga tcgatcattt
    37021 ttttaatcgt cagtttttca cgagagatac gcttgccgga catgctgcct ccatactctt
    37081 aaggtgcatt tatattacaa cttaatttta aaggggacta tggctctgaa ggaggaggtt
    37141 tcttcaaccg acgaatttgg cgatcttgtt tcatcgtcat cacgcaaaag ctgcaaagca
    37201 gcatttttcg cggaaccgac atcaagaact cacctttcgc gtcttcccct gaaatgatta
    37261 actccggtat catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacactacat
    37321 ttaacatcag ggagccggac ttaaccccat gagtacaata gaaaatttcg acgcccatac
    37381 gcccatgatg cagcagtatc tcaagctgaa agcccagcat cccgagatcc tgctgtttta
    37441 ccggatgggc gacttttatg aactgtttta tgacgacgca aaacgggcgt cgcaactgct
    37501 ggatatttca ctgaccaaac gcggtgcttc agcgggagag ccaatcccga tggcggggat
    37561 tccctaccat gcggtggaaa actacctcgc caaactggtg aatcagagcg aatccgtcgc
    37621 tatttgtgaa cagattggcg atccggcgac cagcaaaggg ccggttgagc gcaaagttgt
    37681 ccgtatcgtt acgccgggca ccatcagcga tgaagcgctg ttgcaggaac gtcaggacaa
    37741 cctgctggcg gctatctggc aggacagtaa agggttcggc tacgcaacgc tggatatcag
    37801 ttccggtcgt tttcgcctga gcgaaccggc ggaccgcgaa acgatggcgg cagaactgca
    37861 acgtacgaat ccagcggaat tactgtatgc ggaagatttc gccgagatgt cgctgattga
    37921 aggtcgccgc ggcctgcgtc gtcgcccgct gtgggagttt gaaatcgaca ccgctcgcca
    37981 gcaattgaac ctgcaatttg gcacccgaga tctggtcggt tttggtgtgg agaacgcacc
    38041 acgcggactt tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgcacgac
    38101 cctgccgcat attcgttcta tcactatgga acgtcagcag gacagcatca ttatggatgc
    38161 cgcgacgcgt cgtaacctgg aaattactca gaacctggcc ggcggtgcgg aaaatacgct
    38221 ggcttcagtg ctcgactgca ctgtaacgcc gatgggcagc cgtatgctga aacgctggct
    38281 gcatatgcca gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt
    38341 gcaggatttc accgctgagc tacagccggt actgcgtcag gtcggcgacc tggaacgtat
    38401 tctggcgcgt ctggcgttgc gtaccgctcg cccacgcgat ctggcccgta tgcgtcacgc
    38461 tttccagcaa ctgccagagt tgcgtgcgca gttagaaaat gtcgatagtg caccggtaca
    38521 agcgctgcgt gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat
    38581 catcgacaca ccaccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga
    38641 agagctggat gagtggcggg cgctggctga cggcgcgacc gattatctgg agcgtctgga
    38701 agtccgcgag cgtgaacgta ccggcctgga cacgctgaaa gttggcttta acgcggtgca
    38761 cggctattac attcaaatca gccgtgggca aagccatctg gcaccaatca actacatgcg
    38821 ccgccagacg ctgaaaaacg ccgagcgtta cattattccg gagctgaaag agtacgaaga
    38881 taaagttctc acctcaaaag gcaaagcgct ggcactggaa aaacagcttt atgaagagct
    38941 gttcgacctg ttgctgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcaga
    39001 acttgatgtg ctggttaacc tggcggagcg ggcctatacc ctgaactaca cctgcccgac
    39061 ctttattgat aaacctggca ttcgtattac cgaaggtcgc catccagtgg ttgaacaagt
    39121 actgaatgag ccgtttatcg ctaacccgct gaacctgtcg ccgcagcgtc gcatgttgat
    39181 cattaccggt ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cgttgattgc
    39241 gctgatggcg tatatcggca gctacgtacc ggcgcaaaaa gtcgagattg gcccgattga
    39301 ccgtatcttt acccgcgtag gtgccgcgga tgatctggct tccgggcgtt caacctttat
    39361 ggtggagatg accgaaaccg ccaatatttt acataacgcc accgaatata gtctggtgtt
    39421 gatggatgag atcgggcgtg gaacgtctac ttacgatggt ctgtcgctgg cgtgggcatg
    39481 tgcggaaaat ctggcaaata agatcaaagc gttgacgctg tttgctaccc actatttcga
    39541 gctgacccag ttaccggaga aaatggaagg cgtcgccaac gtgcatctcg atgcactgga
    39601 gcacggcgac accattgcct ttatgcatag cgtgcaggat ggcgcagcga gcaaaagcta
    39661 cggcctggcg gttgcagctc tggcgggtgt gccaaaagag gttattaagc gcgcacggca
    39721 aaaactgcgt gagctggaaa gcatttcgcc gaacgccgct gctacgcaag tggatggtac
    39781 acaaatgtct ttgctgtccg taccggaaga aacttcgcct gcggtcgagg cactggaaaa
    39841 cctcgacccg gattcactga ctccgcgtca ggcgctggaa tggatttatc gcttgaagag
    39901 tctggtgtaa taataattcc cgatagtctt ttgctatcgg gaatattaac aacaactgac
    39961 gaatcaaata aaaataccct gtataatagg aaagcttatt ttacagggta aaaccatgcc
    40021 atctacacgc taccaaaaaa tcaacgccca tcactatcgc catatatgga ccgttggtga
    40081 tattcatggt gactatcaat tattacaatc ccgcttacat caactctctt tttgccctga
    40141 aaccgactta cttatttcgg taggcgataa catcgatcgt gggcccgaga gtcttaacgt
    40201 gctgcgcctg ttaaaccagc cctggtttat ctccgtcaaa ggcaaccacg aagcgatggc
    40261 gcttgatgca ttcgaaactg gcgatggaaa tatgtggctt gccagcggtg gtgactggtt
    40321 tttcgattta aatgattcag agcaacaaga ggcaatagat ctgttgctga aattccatca
    40381 ccttccacat attattgaaa tcactaacga caccataaaa tatgtcatcg cacatgcaga
    40441 ttatccgggg aaggagtatc agtttggcaa agaaatagcg gagagcgaat tactctggcc
    40501 tgttgatcgt gtgcagaaat cacttaatag cgaattacaa aaaataaacg gtgccgactt
    40561 ctttatattt ggtcatatga tgtttgataa cattcagacg ttcgctaacc agatttatat
    40621 agataccgga tcgccgaaaa gcgggcggct gtcattttat aaaataaagt agtctcatgc
    40681 ttcttctgtg aagcatgagt aacccggcgt tattgcaggt cattattcat ttttcgctac
    40741 cagcaaagag agatccagct tcgccagcgc gcggctggca ctctccggca gaccatcgtc
    40801 agtaatgatc tgatcaaact cgcttaatgg taacgccagc catgtcgcca cctgtccata
    40861 tttcgtcgca tcacagacca aaactcgctg gcggctggca ctggcaatcg cccgtttcac
    40921 cgtgacttta tcttctgctg gcgtagagat cccccgcaca ctccatgacg atgcagaaat
    40981 aaaagcctga tcaatcatca ggctacgcag catggtcgca gcggcttccc caacacagga
    41041 acggttttcc cgacacactg cgccgccagt gtgaataatt gtgcaattac tgttgtcgag
    41101 caagtagtcc gcaataacga agtcgttagt aaccactgtg agtggctcca tgtgaatcag
    41161 atgctgtgca atcgctaacg tggtcgttcc cgcatccaga tagatacaac gtcccggctg
    41221 aacaagactt gccgccagct tgccaatagc cgctttttgc gtcattgcca gcgcagtttt
    41281 tacctgatga gaaggttcat gcgccacccg tcccggagac tggacgcccc cggacaccag
    41341 cacaacggct ccctgctgct ccagtttttg taaatcccga cgaatggtca tatgtgacac
    41401 attcattctg tccgttagtt cagcaatact aacaatgcct ttttcagcta ccatctcaag
    41461 gatgatttgg cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc ctgctgacat
    41521 tctacgctat ttgcctgcga aacgtgtgcg gcgcaactaa cgcttagttc acataaaata
    41581 acacacaatg ttaatttttg tgaatcagct caccatatcg ttatctttcc gcgcttatat
    41641 tcacaacatc aaacaaaata tcacttaaat taacaaggag agcagatgaa aacgggatcg
    41701 gagtttcatg tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc actgtcatgt
    41761 gtccgcgcag gtctttctac ctggggcgca gacctgaaca gcaatgcctg cgctacgttg
    41821 aaagaggctg gtgcttgcgg ggtttctgat aacgccgcga cgtttgccga aaaactggac
    41881 gctctgctgg tgctggtggt caatgcgacc caggttaaac aggtgctgtt tggtgaaaaa
    41941 ggcgttgcac aacatctgaa acccggtacg gcagtaatgg tttcttccac tatcgccagt
    42001 gctgatgcgc aagaaattgc taccgctctg gctggattcg gtctggaaat gctggatgcg
    42061 ccggtttctg gtggtgcggt aaaagccgct aacggtgaaa tgactgtcat ggcctccggt
    42121 agcgatattg cctttgaacg actggcaccc gtgctggaag ccgttgccgg aaaagtttat
    42181 cgcatcggtt cagaaccggg actgggttcg accgtaaaaa ttatccacca gttgttagcg
    42241 ggcgtacata ttgctgccgg cgccgaagcg atggcgcttg cagcccgtgc ggggatcccg
    42301 ctggatgtga tgtatgacgt cgtgaccaat gccgccggaa attcatggat gttcgaaaac
    42361 cggatgcgtc atgtggtgga tggcgattac accccgcatt cagccgtcga tatttttgtt
    42421 aaggatcttg gtctggttgc cgataccgcc aaagccctgc acttcccgct gccattggcc
    42481 tcaacagcat tgaatatgtt caccagcgcc agtaacgcgg gttacgggaa agaagacgat
    42541 agcgcagtta tcaagatttt ctctggcatc actctaccgg gagcgaaatc atgatcaaga
    42601 ttggcgttat cgccgatgat tttaccggcg cgacggatat cgccagtttt ctggtggaaa
    42661 acggtctacc aacggtacaa attaacggtg ttccaacagg taaaatgccg gaagcaatcg
    42721 acgcactggt gatcagcctg aaaacgcgct cctgtccggt gcttgaagcc acacagcaat
    42781 cgctggcggc tctgagctgg ttgcaacagc aaggttgcaa acagatttat ttcaaatact
    42841 gctctacttt cgacagtacg gcgaaaggta atatcggccc ggttaccgat gcattaatgg
    42901 atgctctcga cacaccgttt acggtcttct ctccggccct gccggtcaac ggacgtacgg
    42961 tttatcaggg gtatttgttc gtaatgaatc aactgctggc cgaatccggg atgcgccatc
    43021 acccggtaaa tcccatgacc gacagctatc ttccccgtct ggttgaatcg caatccacag
    43081 ggcgctgcgg cgtcgtttca gcacatgttt tcgaacaagg tgtgaaagcc gttcgtcagg
    43141 agctggctcg cttacagcaa gagggctatc gctacgcggt gcttgatgcg ctgaccgaac
    43201 accatctgga aattcagggg gaagccttgc gcgatgcccc actggtaacg ggcggttctg
    43261 gtctggcgat tggccttgcc cggcagtggg cgcaagaaaa cggtaaccag gctcgcaaag
    43321 cagggcatcc gctcgctggg cgcggcgtag tgctctccgg ttcatgctct caaatgacca
    43381 accgccaggt agcacattac cgtcaaattg caccagcccg tgaagttgat gtggcacgct
    43441 gcctctcaat tgaaactctg gccgcttatg cacacgaact ggcagagtgg gttctgggcc
    43501 aggaaagtgt acttgctcca ctggtttttg ccaccgcaag cactgacgca ttggcagcaa
    43561 ttcaacagca atacggtgca caaaaagcca gtcaggcagt agaaacactg ttttctcaac
    43621 tagcggcgcg gttagcagcg gaaggcgtga cacgctttat tgtcgcaggc ggtgagacct
    43681 ccggcgtagt cacacagagc ctgggcataa aagggtttca tattggccca accatttccc
    43741 ctggcgtgcc gtgggtaaac gcactggata agcctgtctc actcgccctt aaatctggca
    43801 acttcggtga tgaagccttt ttttcacgag cccaaagaga gtttttatca tgagcgattt
    43861 cgcaaaagta gagcagtctt tgcgagagga gatgacgcgg attgccagtt cattctttca
    43921 gcgcggctat gcaaccggtt cggctggcaa tctgtcgctg cttttacctg acgggaattt
    43981 actggcgaca ccgacaggtt catgcctggg caatctcgat ccgcagcggc tttccaaagt
    44041 cgccgcggat ggcgaatggt taagtggtga caaaccctcg aaagaggtgc tctttcatct
    44101 ggcgctgtat cgcaacaatc cgcgctgtaa agcggtggtg catttgcaca gcacatggtc
    44161 gacggcgctt tcctgcctgc aagggctgga cagcagcaac gttattcgtc cgttcacacc
    44221 atacgtggtg atgcggatgg gaaatatccc gctggtgcct tattaccgac cgggcgataa
    44281 acgcatcgca caggatctgg cggaactggc agcagacaat caggcttttt tactggcaaa
    44341 tcatggccca gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata tggaagagct
    44401 ggaggaaacg gcaaagctga tttttattct cggtgaccgc ccgatccgtt atctgaccgc
    44461 aggtgaaatt gcggaattaa ggagttaatg caatgcctcg ttttgcagct aatttatcca
    44521 tgatgttcac cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg
    44581 atgctgtgga gtttctgttt ccctatgact actcccccca gcaaatccag cagcaactgg
    44641 tgcaaaacca tctgacactg gcgctgttca ataccgctcc cggagatatt aatgcgggag
    44701 aatggggatt atccgccctt cccggacgtg agcacgaagc acgcgcagac atcgacctgg
    44761 cactggaata tgcattggcg ctcaattgtg aacaagtcca tgtgatggca ggcgtcgtgc
    44821 ccgctggcga agatgctgaa cggtaccggg cagtatttat tgataatctc cgctacgctg
    44881 ccgaccgctt tgcgccacac ggcaagcgaa tattggttga agcactcagc cccggcgtta
    44941 agcctcatta tctcttctcc agccagtatc aggcactggc tattgttgaa gaggttgcgc
    45001 gagataacgt gtttattcaa ctagatactt tccatgcgca aaaagtagat ggcaacctga
    45061 cgcatttaat ccgcgactac gccgggaaat atgcgcacgt acaaattgcc ggactacctg
    45121 atcgacatga accggacgat ggagaaatca actatccgtg gctgttccgc ctgttcgatg
    45181 aagtgggata tcagggctgg atcggttgtg aatacaaacc tcgtggcctc accgaagaag
    45241 ggcttggctg gtttgacgcc tggcgctaat tcgctcaatc cctaattaca acgtacccat
    45301 acatcccccc atctacggat ggggtaggtt aactataact ttcagacagg gtttcccatg
    45361 tccacaatta cattgttatg cattgcgtta gctggcgtaa tcatgctgtt gctactggtc
    45421 atcaaggcaa aggtacaacc attcgttgct ctgctcctcg tcagcctgtt agtcgcactt
    45481 gcggcgggta taccggcggg cgaagtgggt aaagtgatga tcgccgggat gggcggtgtg
    45541 cttggttccg tcactattat tattggtctg ggcgctatgc tggggcgtat gatcgaacac
    45601 tctggtggtg cagagtcact ggctaattat ttcagtcgca agttaggtga caaacgaact
    45661 atcgctgcgc tgactctggc agcgttcttc ctcggtattc ccgtcttctt tgatgtcggc
    45721 tttattattc ttgcgccaat catttacggt tttgccaagg ttgccaaaat atcaccactc
    45781 aaatttggcc tgcctgtcgc tgggatcatg ctcactgttc acgtggcggt accgccgcat
    45841 ccaggccctg tcgccgcagc ggggttactc cacgcagaca tcggctggct aaccatcatc
    45901 ggtattgcga tttctattcc cgtagggatt gttggctact ttgcagcgaa aataatcaat
    45961 aagcgccaat atgcgatgtc ggtcgaggta ctggaacaga tgcaactggc tccggccagt
    46021 gaggaaggcg cgacaaaatt aagcgataaa ataaatccac cgggcgtcgc gctggtcacc
    46081 tcgctaattg ttattcctat cgcgattatc atggcgggta cggtttctgc aacactgatg
    46141 ccgccttcgc atcccctgct tggtacgcta cagttgatcg gctcaccaat ggtagctcta
    46201 atgattgcgc tggtgctggc attctggtta ttggctttac gtcgcggctg gagcttacaa
    46261 cacaccagcg acattatggg ctctgccctt cccactgcgg cggtagtgat tttggttact
    46321 ggtgctggag gggtatttgg caaagtgctg gtggaatcgg gcgtcggtaa agcccttgcc
    46381 aacatgttac aaatgattga cctgcctctg ttaccagccg catttattat ttcactggcg
    46441 ctgcgtgcat cgcaggggtc agcaaccgta gcaatcctga caaccggcgg gttactctca
    46501 gaagcggtga tgggattaaa tccgattcag tgcgtattgg tgacgctggc agcctgcttt
    46561 ggtgggcttg gcgcgtcgca tattaatgac tcagggttct ggattgtgac caaatatctg
    46621 gggttgtcgg tagcagacgg tctgaaaacc tggacagtgt taacgaccat tctcggtttt
    46681 accggattct taattacctg gtgcgtatgg ctcgtcatct aataatccag ataagatatg
    46741 atgtatcgtt taagtgcctg ataagggtgt aatgtatgcc aatattaacc tcggtaatat
    46801 ctcgctaccg aggttaattt tcactttgca atataattac agtctcttgc aagttgattt
    46861 tatcttagta ataagaaccg actaatctta ttgctatttc gatattagca ggcatagatg
    46921 cgtcgtagcc atacatatgg ccatatttcg gacgtaatac ttcagttact ttcttaataa
    46981 ttgcttcgcg ctgggcccgc gcaaaatcag catgattatt tgccgtttta ttcataaatt
    47041 taccaaaact ttcttctttt ctgactttaa ttgtcgtatc cctaagatca ttaagaaact
    47101 ggcgttgaaa tttgaaatca tcgtgtgaac caatattacc atgcccgcca ataaaatatt
    47161 tccagtcaag catttcaacc tgacgcaaat tactgtcatg gtaaaccaac gtatccgata
    47221 cggcgaagcc catcatcggc agttggtcag gattcaggag atccgggctg tgagctactt
    47281 tttcctgttt tagcaaccat acagaatggt cgtcggtatg tcctgcttta acaaatcggt
    47341 gtaattcaat ggtctgcttt tcaaatttaa actggtcatc ttttttactt aaaacctgag
    47401 tggcgacggg taagcgactg ttcataaatt gcattttttc agcagtggct ttactggcaa
    47461 taatacgcag attcacgcct tctttcttcg cctcatcgtt ccaaaatgga ctatcgccaa
    47521 tgtgatccac atggtaatga gaataaacca gcgctgttac gggtaatgga gtcacatcac
    47581 gaattgcctg taataaatat ttaccgcgac cctccggagc atcaaacagc aaaacccctt
    47641 tgtctccgac ataaaacgta gtggaataaa aatagcgttg aacataataa gttctctcgc
    47701 caattttttg taaaatataa ggctgagaca tatttctttc aaaaaggtta ttgagtttat
    47761 cgcttggcga tattattttt ttcactgaag attcaacata acttgcatta actgtagacg
    47821 aatttgcagt cgaaatttcc gtttgagcaa aaatactacc agatataaga gaagaggcta
    47881 gaattgtata tattttaatg ccttgcatac gtagtccttt attaatgtgt tgttgattta
    47941 tagcatctct gaacagactg ttattttaga tattactacg ttacgagatt catttttctt
    48001 cgaatattat gtttatctca ttcaaaaaat atgttcatca tttgatggtt taaataatat
    48061 ctgcaaggca taatactcca catctttaaa acctgcctca aaaaatcatt cattagtaaa
    48121 aaggatattt atatgcaaaa catagctgtg gtttattatt caggatatgg tcacactaaa
    48181 ctggttgctg aaaaagtggc acaaggcgcc catgcagatt taatttcgat tgataacgaa
    48241 ggaaatatca ctgagcagga gtgggaaaaa ctggacgcag ccgatgccat tatcttcggt
    48301 gctcccacct atatgggcgg tgcgccatgg caattcaaga aatttgctga tgccagttcc
    48361 aaagcgtggt ttacacgtaa atggcaggat aaagtcttcg ggggtttcac caacagcgcc
    48421 agcctgaatg gcgataaaca agttacgctg atctatctgc aaacgctggc ttcacagcat
    48481 ggcggtctgt gggggagttt aggtcaggct cctgccaatg tgcttgcgtc aaccagggaa
    48541 gatgtaaaca atctgggcgg ttcagtcggt ttgctggtgc aatctccagc cgatgcgggt
    48601 gcagaccaaa tccctacggg cgatctggat acagccgtga aatatggtga acgcgtggcg
    48661 acaattactg cgcgcttaaa atgatgattg ccgaatgtga cgctgacgcg tctatccggt
    48721 cttctacatc taaaaagtgc cgtcccctgg tcagtcagat aaagttttaa cgctgtatcc
    48781 gataacagtt caacatgtac caaaagaaaa aaagccagcc tcgcttgaga ctggcctttc
    48841 tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg cgtttgcagg
    48901 atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc acgggtgagg
    48961 ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa accgaatcga
    49021 cgtgccagta cttcacgctg tttggcgttc agctcgaaca gccatttgac gatgctctgc
    49081 ttcatatcgt catcttgcgt ggtatcttcc ggaccattct ctttttcatc ggccaggatg
    49141 tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt aatgcgctcg
    49201 ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc tgcgatctct
    49261 tccgcacttg gttcgtggtc cagcttatgg gacaactcac gtgcggttcg caggtaaacg
    49321 ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt cataatcgcc
    49381 cgttcgatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa accacgttcc
    49441 gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat caggtccagc
    49501 aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg caagttactc
    49561 tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc aaaataaact
    49621 tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag ctgagtcgcg
    49681 tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa atcgttatca
    49741 ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt ctcatcaaat
    49801 tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag gtggctccta
    49861 cccgtgatcc cttgacggaa cattcaagca aaagcctggt tccgccgatt tatcgctgcg
    49921 gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa tgcaagcgtg
    49981 ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc acttcttgtt
    50041 gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca tcattatgtt
    50101 tgatgataat cagattaccg tagccgcgca gcgcgttacc ggcataaaca acgcggccat
    50161 ccgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc ttgttgcccc
    50221 cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc caggtggaga
    50281 taggcgtact ggttgatgta ctgctgacag tcggctcggt tgtgcttgct gttggtaccg
    50341 ttacaggcgc tgtgaccgtg gtcgcagttg gcttgttgtt cggcaacatt ttgttagcac
    50401 tctgttcacc cgaagactca gaatacgtaa ttgtcggttg cgacgcaaca gcaacggtgg
    50461 aattttgtgc aggcttgatc acaactcctt gctctgctgc gtcggcctgg gtaatggcat
    50521 ttccgcctgt gattggcgta ccggaagcat tacccacctg taaggtctga ccgacgttca
    50581 gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg ttgccagtaa
    50641 tccaggcgat atagaaaagc gtgtcgcctt ttttcacggt gtatgtactg ccgctataac
    50701 tgcctttcgg aatgttccca tactgacggt tgtagacgat tcgtccgttt tccatctgta
    50761 ccggctgctg agctactggc tgcacaggct ggatttgcgg ttgttgagta gcctgaattt
    50821 gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtc cccattttcg
    50881 gcggcggcgt aatcaacata ccagaattgg tatttgcggg cgcattgcca ttaacggagc
    50941 tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc gaaaccagtg
    51001 acaatgccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt tatcccccag
    51061 gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccatac tgaacactgg
    51121 aaaagatgtt cacgatacgc tgacttgcgg caaaataacc aggaaaaatc caggtatttc
    51181 ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc ctccacggta
    51241 tcgataataa attcgcctcc ccgacgacgc acccgtttta aatactggtg ctcctcccct
    51301 acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt tggaatttca
    51361 ggcggtgccg ccgtaacaat gatagcgtca aacggcgcgc gtgcctgcca accttgccat
    51421 ccatcgccat gacgggttga aacattatgt aaatcaagat ttttcaggcg gcgacgcgcc
    51481 tgccactgca agcctttaat ccgttcaacc gagcaaacat gttggacaag atgcgccagg
    51541 attgcagttt gatatcccga accagtgcca atttccagca cccgcgactg cggcgtcagc
    51601 tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg cccctgacct
    51661 atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac gaatttttca
    51721 cgcggcacgg cggcaagtgc attcagcacc agctcatcct gaataccttg cgcacgtaat
    51781 tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc acgctgttta
    51841 accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc agcggcgtga
    51901 tggaaacata gccctcatct accgcagcaa aatcggtccc cggaccggca tcacatttac
    51961 cgcccggcgg gccaatccag tacagcgtat taccgcgcgg atcttgctgc gggatcacct
    52021 gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt tgatccaagg
    52081 gtaaatccgg aacgttaata ttaagaatac gcccggtgcg cagcggctct ttacacagtg
    52141 cgcgcaaaat tgagcaggtt accgccgcgg cagtgtcgta atgtttatgc ccgtcaagcg
    52201 agacggcaag cgccggaaaa cctaaatgac ggccttccat cgcggccgct accgtaccgg
    52261 aataaataac atcatccccc agattcggcc cggcgttaat tccggacaca acaatgtccg
    52321 ggtgcgggcg catcagagca tttacgccaa gatagacgca atcggtcggg gttcccattt
    52381 gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc agtgtcagag
    52441 aattagaagc gccgctgcgg ttacgatcgg gggcgaccac ctgaacgtca gcaaactcac
    52501 gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca ttactcagca
    52561 atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt tgcaaaactt
    52621 cccgccggaa gccagaaacg gatttctacg gtgacatcat cccaccagtt ccagcttaat
    52681 tgttgcggat acagcagcat cgctctgcgc gcggcttcaa ctttttcgcg caccagtaag
    52741 gtttgtaatt cagtttcttc ggcgacagct gcttgttcga atgccagcgc ttcacgctga
    52801 gttccccact cgccactgcc cggcaatgcg gcggttatca tcagcacttt atcgttgacg
    52861 cgacgctgta attccgccag ttcttcggtg gttgcgacaa accagctacc acgtccggct
    52921 aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag gcgttcagca
    52981 acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg tttattgcga
    53041 tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcac cctgcaagtt gctaccgcca
    53101 atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat atcgatcaga
    53161 cgttgttcaa cgtcatcgcg attgctcact tcgcgtagta ccagggtaaa ggcgttacct
    53221 ttcagcgccc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac ctggcagcct
    53281 tccagttgaa aggcgctcag atcgggcatc tctttgcccg gcacgcgagc gcataaccac
    53341 tgttccgtaa cagcatgttt atctttttgc ccagcgaagc tgacttcacg ggcatgaatt
    53401 ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt tttgaggatt
    53461 ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc caccaccaca
    53521 aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg gaggtaagtg
    53581 agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct tcacaggcaa
    53641 tcccttcccc acgtccggta aatcccagtt tttccgtagt agtggctttc acgttaacat
    53701 catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga atgtgcggca
    53761 acatcttcgg tgcctgagcg atgatagtga catcgacgtt gccgagggca taacctttcg
    53821 cctgaatacg acgccaggct tcgcgcaaca gctcgcggct gtcggcacct ttaaatgccg
    53881 gatcggtatc cgggaacagc ttgcctatat ctcccagcgc cgccgcgcca agcaatgcat
    53941 cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc cagcagccct ttttcgtaag
    54001 gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcgtgt acgtcaaaac
    54061 cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt aaaattcggc
    54121 cagcgccaaa tcctccgggc gcgttacctt gatgttatcc gcgcggcctt caacaagttg
    54181 cggatgaaaa ccgcaatatt ccagcgccga ggcctcatcg gtaatggtag cgccttcatt
    54241 tagagcgcgc gtcaggcagt catgtaacag ctcacgaggg aaaaattgcg gcgtcagcgc
    54301 gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg gttcggcacg
    54361 tttcatcgta tcgcgcaccg gcgcagcgag gatccctccc gtgcggctgg tttcgctcaa
    54421 cgccaacaat cgcgcgaggt catcctgatg cagacaagga cgagcggcgt catgcaccaa
    54481 tacccactgc gcgtcgccag cggctttcag acctgccagc acggaatcgg cacgctcttc
    54541 accaccatca acaaccgtga ttcgtggatg attcgccaga ggaagttgtg caaagcggct
    54601 atcgccagga cttatggcaa tgacgacacg tttcaccctg ggatgcgcca gcagcgcatg
    54661 caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag gacattccgt
    54721 ttgcattcgg cggccaaatc cggccgccgg aaccacggcg caaacatcca aatgagtggt
    54781 tgccatgtta attcccgggc tgatttatcg attattttgc cccgcagact gtgcgcgctt
    54841 tgacgcgtca ggcaccagac gataaaacgt ttcgcccggc ctggtcatgc tgagttcatt
    54901 acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt cggcaaaaag
    54961 ctgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc tgtgccgcca catcattatt
    55021 gacgcgagta tagtcatgta taccgttctt accgaaccac agcgaatact gtagccagac
    55081 cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa aacggcatct
    55141 tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc gacaacgcgg
    55201 gcaaatgtac cacatttgtc gattgttacg tatacccagg gcgtgcagaa cataatctca
    55261 ttattagtta cggattgaat tatgaacaga atagagagga aagaacaatt aacccagtag
    55321 ccacataaac agtgcaccaa acataatgcc aaccgtcgcc agagtgaaaa caatactgta
    55381 gcgtagcttt ccgtccatca atgaatgcag tgcaatcccc accactacag cgacgggcat
    55441 cagcgccaga aagaaaggcc aggtgtagat aaaaaagagc agcgtgttag agccataaat
    55501 caacatcgga attgccaacg cgcacagcca cgataaaaaa ccaaccacag cgccaggcag
    55561 cgaccatgtg gtgtcttcaa cctcagtaag gctgtcgtta tttattaatg taatgttgtg
    55621 gctgttacgc atatctgatc ctgttacttt gacgaaccgg gcatgaaaac ccggtggtgt
    55681 ctcaggatct gataatatcg ttctgtctca acagatctaa taattgctgt accaaatttg
    55741 ttactaattg ttcaccattg agatgaattt ctgccgattc aggcgcttcg taaacggaat
    55801 ctattcccgt aaagttgcgc agttcaccgg cacgcgcttt cttatataaa ccttttggat
    55861 cgcgggcttc gcaaatcgcc agcggcgtat cgacaaacac ttcgataaag cgcccttctc
    55921 ctacgcgttc gcgaaccatc tggcgttcgg cgcggtgtgg cgagataaat gcggtcagca
    55981 ccaccagtcc ggcttcaacc atcaaattcg ccacttcacc gacgcgacgg atattctctt
    56041 tacggtcggc atcgctaaaa ccgagatcgc tgcataatcc gtggcgaacg ttgtcgccat
    56101 ccagcagata cgtactgacg ccaagtttat gtaacgcctc ctccagcgcc cccgcgacgg
    56161 ttgatttacc ggaaccagag aggccggtaa accacagcac aacaccacga tgaccgtggt
    56221 gctgttcgcg ttgttgcgga gttactgggt ggctatgcca gacgacgttt tcgtcatgca
    56281 gagccattat ttatctccca gtaaatcgcg cgcaccccaa tgcgggaaat ggcggcgaac
    56341 cagggcattc aattccagtt cgaatgcact gaattcagat ggcgcagcag ttgcctggct
    56401 aactggttcg tgcaccatac cggcacctac ggtcacatta ctcaggcgat cgataaaaat
    56461 cagcccaccg gtaaccgggt tttgctgata acgatctaac accagtggct cgtcaaaggt
    56521 gagatctacc aggccgatgc cgttcagcgg caggttttca acttcgcgtt gggtaaggtt
    56581 attgatatca acctgataat gaatgccatc aacacgagca cgcgtcttct taccggcaat
    56641 tttgatgtcg taactctggc ccggggaaag cggctgttcc gccatccata ccacatccac
    56701 cgacgcgctc tgcacagccg gtaacgcttc gtctgccgcc agcagcagat cgccacggct
    56761 gatgtcgatc tcatccgtca gcaccagggt gatcgcttct ccggcaaagg cttcttcgcg
    56821 atcgccatca aaggtcacga tccgcgcgac gtttgattcc acaccagagg gcagcacttt
    56881 gacacgttgc ccgacttcca cacgaccgga tgccagcgtt ccggcgtagc cacgaaaatc
    56941 gagattcgga cggttaacgt actgcaccgg gaagcgcatt ggctgagcat ccaccactcg
    57001 ctggatctcc acggtttcca gcacttctag cagtgtcgga ccgctgtacc acggcatact
    57061 ttcactttgc gaagccacgt tgtcgccttc cagtgcggag agcggcacaa agcggatatc
    57121 cagattaccc ggcagctgcc cggcaaaagt cagataatct tcacgaatac gggtgaacgt
    57181 ctcttcactg taatccacca gatccatttt gttgatcgcc acgaccagat gtttgatccc
    57241 caacagtgtg gagataaaac tgtgacgacg ggtttgatcg agcacgcctt tacgggcatc
    57301 gatcagtaag atcgccagtt cacatgtcga tgcgccagtc gccatattgc gggtgtactg
    57361 ctcgtgccct ggggtgtcgg cgataataaa tttacgcttc tcggtagaga aatagcggta
    57421 ggccacatca atagtgatgc cctgttcgcg ctcagcttgc aggccgtcca ccagcagtgc
    57481 cagatccagc ttttcgccct gggtgccgtg acgcttactg tcgttatgca gcgatgagag
    57541 ctgatcttcg tagatttggc gggtatcgtg cagcaggcga ccaatcaggg tacttttgcc
    57601 gtcatcgacg ctaccacagg tcagaaaacg cagcaggctt ttatgttgtt gcgcaatcat
    57661 ccaggcttcg acgccgcctt cattggcgat ttgttgtgca agtgcggtgt tcatcttaaa
    57721 aatacccctg acgttttttc agctccatag accccgcctg gtcgcggtca attacgcggc
    57781 cctgacgttc actggtggtg gaaaccagca tctcttcgat gatctccggc agtgtttgcg
    57841 catttgactc caccgcgccg gtcagcggcc agcagcccag cgtacggaaa cgcaccatcc
    57901 gttttttaat cacttcgccc ggttgcaggt cgatacggtt gtcatcaatc atcatcaaca
    57961 taccgtcgcg ttccagaacc ggacgttccg cagcaagata cagcggaaca atgtcgatat
    58021 tttccagcca gatgtattgc cagatatcct gctcggtcca gttagagagc gggaagacgc
    58081 ggatgctttc gcctttgtta atttgcccgt tgtagttgtg ccacagctcc gggcgctgat
    58141 ttttcggatc ccagcgatgg aagcggtcac ggaaagagta gattcgctct ttagcgcggg
    58201 atttctcttc gtcacggcgc gcaccaccga aggcggcatc aaaaccgtat ttgttcagcg
    58261 cctgtttcag gccttcggtt ttcataatat cggtatgttt cgcgctgccg tgtacgaatg
    58321 gattaatccc catcgccacg ccttccgggt ttttatgcac cagcagctcg cagccgtagg
    58381 ctttcgccgt acgatcgcgg aactcataca tctcgcggaa tttccagccg gtatcgacat
    58441 gcagcaacgg gaaaggcagc gtacctggat aaaacgcctt gcgcgccaga tgcagcatga
    58501 cgctggaatc tttaccgata gagtagagca tcaccggatt tgagaattct gcagccacct
    58561 cgcgaataat gtggatgctt tccgcctcca gttgccgcaa gtgggtaagt cgtttttgat
    58621 ccataaccgt tcctttgcaa taccgctatt ttcttgccat cagatgtttc gactataggg
    58681 agcgtaagag aacgaatgaa attaccaatt agaatgagta gttccttaac ggaataacga
    58741 tttggcaaag ctaatatcaa aaagtgctta aggcaccgga tttcgggcgt ttaggaagat
    58801 ttgaaattgt tttagcgcag cggcagtttc atactatggc ggtaaaaaaa tttgcatggt
    58861 atttaaggac tcactatgtt ttccgcattg cgccaccgta ccgctgccct ggcgctcggc
    58921 gtatgcttta ttctccccgt acacgcctcg tcacctaaac ctggcgattt tgctaatacc
    58981 caggcacgac atattgctac tttctttccg ggacgcatga ccggaactcc tgcagaaatg
    59041 ttatctgccg attatattcg ccaacagttt cagcaaatgg gttatcgcag tgatattcgg
    59101 acatttaata gtcggtatat ttataccgcc cgcgataatc gcaagagctg gcataacgtg
    59161 acgggaagta cggtgattgc cgctcatgaa ggcaaagcgc cgcagcagat catcattatg
    59221 gcgcatctgg atacttacgc cccgctgagc gatgctgacg ccgatgccaa tctcggcggg
    59281 ctaacgttac aaggaatgga tgataacgcc gcaggtttag gtgtcatgct ggaactggct
    59341 gaatgcctga aaaatacgcc taccgagtat ggtattcgat ttgtggcgac cagcggcgaa
    59401 gaggaaggaa aattaggcgc tgagaattta ctcaagcgga tgagtgacac cgaaaagaaa
    59461 aatacgctgc tggtgatcaa tctcgataac ttaattgttg gcgataagct gtatttcaac
    59521 agtggcgtaa aaacacctga agcagtaagg aaattaacgc gcgaccgggc gctggcaatt
    59581 gcccgcagcc atggaattgc cgccaccaca aatccaggtt tgaataaaaa ctatccgaaa
    59641 ggcaccgggt gttgtaatga cgcagaaata ttcgacaaag ccggtattgc tgtactttcg
    59701 gtggaagcga ctaactggaa tcttggcaat aaggatggtt atcagcaacg cgcaaaaaca
    59761 gccgcattcc ctgcaggaaa tagctggcat gacgtaagac tggataatca acagcatatt
    59821 gataaggcac ttcctggaag aatagaacgc cgctgccgtg atgttatgcg gataatgcta
    59881 ccgttggtga aggagctggc gaaggccatc ttaatgggtt tgaaatgatt actcccttgc
    59941 ctgcaggaaa tcaatgccac cagaaacaga gtataaaaaa gggtgcagga gaccaaacca
    60001 tcacaattca gtacgcgggg ttttaccccg cgtggtggct acttcttcga ttcgtaagcg
    60061 agaactgcct taaactccat accacgttct ctgatactca actcacacaa cgagtcacct
    60121 accatcaacg tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat
    60181 tttgtcagca tgtcttgcct ccctgcaaag aagagactaa catccgaatt gctacgttcg
    60241 aacgcgagcc tcaggttgat agaaatatcg cctggggctt ttgtccatct ggaacctcgc
    60301 gaatgcttaa cgccagacag cctcaagcac ccaatgccat tctatacctg ttaattctcc
    60361 ctgcgacgtt ctcgtttaaa tgccggaatg ctgctcgcaa aataaaaacg ccccatcatt
    60421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgtaatc cacattccct
    60481 ttttaagcca aagaaacgcg tttcttcttc cgctatgccg ggttcccatt tacgggttgt
    60541 atgggtatcg cccaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
    60601 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
    60661 tacgccacgc tgaattgcca gcaccggtaa attggcgcga ctgccggatt gttcacggcg
    60721 caggccagca aaccaggttt gcgcattcag ttctttcagc gcccggttca tcggttcgac
    60781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgctcccaca gtttgccgta
    60841 gcgtgcttcc tgccaggcgg cgctttcggt agcacggtac actttcaggt tgagcttgag
    60901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtatcggt
    60961 gaggatcact ggaatatccg ggcgaatctg attcaccaga tgcagactca ccgccgcctg
    61021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggctac
    61081 gcggccttca gcgtccagtt tttccagttg ggcgttagtt tccgccagcg ccagaatccg
    61141 gtctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacataa gttcctcact
    61201 gtttgccttg cctgatgcaa cgcttacaca tcttatcagg cctacaagac cgggctgatg
    61261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
    61321 taaagtcgcc gaagccttca cccacttcgc gctctttcgc ccagcgccct atcagttcat
    61381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
    61441 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggcgctttc cccaccagac
    61501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
    61561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
    61621 gcaggaaacg ctccgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
    61681 ttgagttttc acgctgcggc gtgacggcat tcattaaccc gctttctttg gcgatcttct
    61741 cgatcttcgc tttctcgctt tccggcacgc cggcgatgat cagattctgg ttcgccgtaa
    61801 tgcggaaatc acctttgtgg atcttcgcga tctccagcaa gccggttttc agcggacgtc
    61861 ccggataatc aagaatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
    61921 tgcccttaac ccagccaata cgatctccgc gtccggtaaa ctcatacggg cgaatcggct
    61981 cgaatttgat ccccgcgcga cgttcaactt ccgctttaaa cgtctcaacc cccacgcgct
    62041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
    62101 ttgtcacgac ggcttccgcc accgccagcg tatgctccag cggcagatag ccaaactcac
    62161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat ggaaagccca ccgcccacca
    62221 gcaggttaaa gcccaccagc ttgccgtttt cggcgatcgc cacgaagttc atgtcgttgg
    62281 cgtgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
    62341 ggtaggtctg accaaggatc ggctcttcat cagtagtggc gactttttcc tgatcgagcc
    62401 agatctccgc atacgcgcgg gtacgaggca gcagatgttc agaaattttc ttcgcccatt
    62461 catatgcttc cgcatgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
    62521 tcatgtcgtt ggcggtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
    62581 gtttgacgtt ctttttcaga atgccgtgga actggaatgt ctggcggttg gtcaggcgaa
    62641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
    62701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
    62761 gttcagcacg ttcggcgcga atgtcgcggt catcctgctg atacatgccg tggaagcgga
    62821 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
    62881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
    62941 cgaccactaa aggccctgga tgtttttcac tcattagtag acatctcgct gataacggcg
    63001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
    63061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
    63121 gacataaatg tgggcaccgt cattgatcca gcgccacagc tctgcgccct gttcgcgcag
    63181 tttgtcttgt acgtaaattt tttctttttg atcgcgcgac caggcgagat cgatgcgcgt
    63241 cagcacgcct tctttgacgt aacgctgcca ttccacctgg tagaggaaat cttccgtaaa
    63301 gtgtgggttg ccaaagaata gccagttttt acccggcgct tcgtcggcag cacgctgctg
    63361 cataaaggca cggaacggcg cgatgccggt gccagggcca atcatgatca ccggggtttc
    63421 cggattggtt ggcaggcgga agttgtcgtt atgttcgata aagacgcgga cttcaccctc
    63481 ttcttccaca cgatccgcga ggaagctgga ggcaccaccg gcacgggcgc ggccttccac
    63541 gtcgtaacgc accacaccaa cggtaacgtg cacttcgttc tcgacttccg cctgcgagga
    63601 ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc
    63661 cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
    63721 taacttcgct ttatcgccca ccagcggcag cagcgtttcg ctgcgggtaa gcgtggcata
    63781 gttctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
    63841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
    63901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccaacg cgtcacccgg
    63961 ctggtaacgc aggccagagt cacctaagtc aatttcaatg tgacgaacgt ctttttcaga
    64021 gttacgcccg gtaattttct ggttaaccga aagactggcc gccagcggcg cgtctttgct
    64081 atacgggctg gtgtggattt catttaccgt gccagtagcg acggattgcg aaggtgccgc
    64141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
    64201 ctggtattca acatcggcat cgacacggtc tagcaggcgt tcaccgccca gttccgccag
    64261 cttactgtca aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
    64321 aaacacggca aacgcggtgt tttccagctt tggcgctttt ttggagaaca ggaacttatg
    64381 cagcgcgacg gcttcttccg gcggttcccc ttccccttgc gttgacgtca ccacgataag
    64441 cagtttttcg ctggcgattt gtttgaattt atagtcaccc gcgttcacca gcttaacgtt
    64501 cagttttgcc gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
    64561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gcgggcgctg gcgtcgctgc
    64621 aagcgcagca ggctgctggt tgagcacgcc ccagaaatag ccagaaaccc aggcaagctg
    64681 ggtaggagtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
    64741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgttgt tatgttccag taagcaaagc
    64801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtaaa
    64861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
    64921 caatagtctt taacaacaaa atagattaac caacccaatg aaaaacaaat gaatattgcc
    64981 catcaataag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
    65041 cagcctttcc ggtactctac ggcggtttta tcgtcatgta gagaaattat gatgtccacc
    65101 acgttattta aagatttcac cttcgaagcc gctcaccgct tgccgcacgt cccacaaggg
    65161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
    65221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac tgaaagcggc gtttaaacca
    65281 acttatgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc
    65341 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
    65401 gcggtgatgg taaaagaaac ctgcaccgca ggttgtatct atcgcggcga atgataagag
    65461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
    65521 tggggcatga catggaagac gactgcgaca ttattattat cggtgccggt attgctggca
    65581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgagcgg
    65641 agattcccgg cagcaaaaat ctttccggcg ggcggctata cacccatgcg ctcgcggaac
    65701 tcctcccgca atttcacctg accgcgcctc ttgaacgacg catcactcac gaaagtcttt
    65761 ctctgttaac gccagatggc gcaacgacgt tttccagctt actgcccggc ggtgaatcct
    65821 ggagtgtatt acgcgcacga ttcgatccgt ggctggttgc cgaagccgaa aaagaaggtg
    65881 tcgaatgcat ccccggtgcg acggtagatg cactgtatga agaaaacggc agggtgtgtg
    65941 gtgtcatttg tggtgacgat attcttcgcg cccgttatgt ggtgctggca gaaggtgcca
    66001 acagcgtcct ggcggaacgc cacgggttag tgactcgtcc tgctggcgaa gcgatggcat
    66061 tggggatcaa agaagtgctg acgctggaac catccgctat tgaagaacgt tttcatctgg
    66121 agaataacga aggcgcagcg ttgttgttca gcggcgggat ctgtgatgac ttacccggcg
    66181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctcct
    66241 cccttacgca aagtcgtgtt ccggcaagcg agctgctggc tcgctttaaa acgcatccgg
    66301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
    66361 aaggtggcct gcgcagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
    66421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctaa
    66481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccaacaccgc gagccgcaaa
    66541 atctgtttcc gctttatcat cacaacgtag agcgcagcct gctgtgggat gttctacagc
    66601 gttatcagca tgttccggcg cttttgcaac gtcctggctg gtatcgggtg tggcctgggt
    66661 taatgcagga tatttcccgt gatttatggg atcaggggaa tacacctgtt ccaccgctgc
    66721 gccagttgct ctggcgtcat ttacgtcgtc acggcttgtg gcatctgacg ggcgatgtta
    66781 tcaggagtct acgatgtctg tagcccgtaa tctctggcgc gctgccgatg cgccgcacat
    66841 tgttccagct gactccgttg agcgccagac ggcacaacgg ttgattaacg cctgtccggc
    66901 tggtcttttt tcgctcacac ctgaaggtga cttacgcgtt gattatcacg gctgcctgga
    66961 gtgtggcacc tgccgtttgc tgtgcgacga atcaacacta caacagtggc gctatccgcc
    67021 ttccggattc ggcatcacct accgctttgg ataaataaca aggataattt atgtccctct
    67081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
    67141 tggcaatcga ctctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatta
    67201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
    67261 tggaaggcgc gtcaaataaa gaagtggtga ttcagttttt gaaactggtg accgaggcgt
    67321 acggaattat cagtaccaaa gcctcaatgc tgaaagcagc aagagcggaa ggttttttct
    67381 gtattcatcg actgtttatt gttgattcga tttcgtttca caacattgat aagcaagtta
    67441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg catgcctaaa gtgctgggct
    67501 gggtgacgga gaaaatccgc caaccgctga ttgctggcgg gctggtgtgc gatgaagaag
    67561 atgcgcgtaa tgcgattaac gcgggtgttg tggcgctttc caccacgaat actggggtct
    67621 ggacgttagc gaaaaaattg ctttgacggg ataatcgtaa caagttgaat ttggtgtgat
    67681 ttgtaggctg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
    67741 caggcctaca agtccccagc ccagaaccat cggcaggatc atccggcagc acgttattgg
    67801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
    67861 ccaacatctg cctgtgaaaa taccgctgca ctggcgtcat gattaatcgc caccacaaac
    67921 ttgctattgc gtacgcccgc cattaacgct gctgcgccgg aagcgccaac aacaatacac
    67981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcggcatc aacgccaccg
    68041 ttcatcactc gtgcccgact gtagcccact tcggctccca tcttttctgc cagcatagcg
    68101 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
    68161 aatgggtcac gggtaacatt tttcaggttt tcgacgctga taagccagtc tggaagcgcg
    68221 ccggggacaa tgttcagttg ctgcatccca aagggcaacg tggcgttttt atctgctccc
    68281 gcctgacgtg ccagcgaaag gcatagcggg cgtttttctg tttgcagcgt cgccgtcaac
    68341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
    68401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgcg
    68461 cccggaggaa aaaggacaac ctccgcaggc gttcgttgcc actgttccac cagcgcatcc
    68521 agcacctgtt ccgcaaccat cggctgtggt tctatttgcc aatgcgttag tgtgctgccg
    68581 ctgaaatcct gcgccgccag ccagcccgcc attgcggcat gttcctgatt tattgtcaca
    68641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ctgccatagc ttttgcgctt
    68701 tttctgctac cgtttgccca tcaatcagcg ccgctctgcg tcgttgctct ggccttgcca
    68761 gttgcaggca ctgtatcgct ggcgcttccg cggcaacagt ttcgcgggta atctccgctt
    68821 tccccgtcgc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccacactgac
    68881 gcacggcaat caccgcaggc aagcgaaccc ggcaacagcg cagtccatgt tcagtacgct
    68941 gttcgagggt gataaacggc gcgtcgagag taaaacgctc cacctgggtg aagcagggcc
    69001 aaaccagcat ttccgccaac aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
    69061 agccagtaat tatcagatcc agcggattct ggtactgcca ttcggcaata tggcgagcga
    69121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
    69181 caagtgccat gagatagcgc agccagtgca acgcccgttc atcccccatg ctcaacgcgg
    69241 ttaaagacat cggcgtaccg ttttttctct gcaccaacag cagcgcggca gcggcctgtt
    69301 catcagcacc gagtaaactt cgcagtagcg aaacatccgg tccgctatta ccctgagccg
    69361 ccgccagcca ctctttttcc gccagcatcc cggcatccgg ttcggcttta aacgctaaca
    69421 gtatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagtgc tttggtttct
    69481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgccgccag gagcaataag
    69541 gtggcttgca ttccccactg tgccagcacc cacggcagca ggccagtact taccgccgcc
    69601 cccaaccgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
    69661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
    69721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccatcac taccagcgtt
    69781 gccgccagca gcaaaaaact tcccagtaaa aatctgcgat gcgctaacaa gtgggtcaac
    69841 actaacccta ataacgcgcc aacaattaac aacgcattaa gcatcaggct ggcagtcagc
    69901 gcatcttcca gaccaatagt ttgggcgatg gtcggcagcc aggtataaat cacaaaccat
    69961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtagcgcgaa
    70021 gagaacaagg ttttgatgtg tttatgggtc gccgttgcca cttcatcgcc cagtaaaaca
    70081 tgcgggccaa aatagcgatg aacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    70141 agccagcgtg gcgattccgg cgtgccccaa cgtagcagcg taatcagcaa ggctagtaac
    70201 gccgctgatg ccagcagcca gcgtggcgat tccggcgtgc cccaacgtag cagcgtaatc
    70261 agcaaggctg gtaacgccgc tgatgccagc agccagcgcc aggcctccgg gctttcggaa
    70321 ataaagtgat gtccggcaat acttgccagc acatagccta cggtccacac cacactgaat
    70381 gcgcccagca aaataccgcg atggcggcgc ggggaaaatt cagccagcaa ggtgtgaccg
    70441 actgagtaat cgcctcccag cccaatgccg atcaaaatgc gcaatccaat aagatgctct
    70501 ggcgtggtgg caaaaaactg taaaaacgaa gcaagcgtaa tcagcataaa actgaaggtg
    70561 aagatttttt gccgcccgat atggtcggag atccacccca aaaccaggct gccaagaaac
    70621 aaaccaagta acgccgagcc gccgatcatt cccgccataa acggcgtcag ttgcatggcg
    70681 ggcgtaagct gaataatggc gtaaccaata acgccgagga cataaccgtc ggtcaggtgt
    70741 gcgccgaaag tgagcgcagc aattcgacag tgaaaacggt taagcggtaa atcatccatt
    70801 cgcaccggtg aagtgttcat ctttacccct tcattgtccg gtaataagaa cggcttctcg
    70861 cctgagaagc cgaaaaggca acgccccgtc atcgcggaac gctgcctgac acattatttt
    70921 tcaatcggat agatagtacc cgtgttcata atgccattag gatcgaactg ctttttcagc
    70981 ccttccagca acgcccacgc gctgccgtgt tccagtttgc tccagtgaac gcgatgttta
    71041 ccgataccgt ggtggtgcac catcgaaccg ccgagacgaa tggtttcttc gcagatgatt
    71101 ttgttgagcg gattgtggta cttgtcgatt tcctcttccg gcttacagtc aacgacgttg
    71161 tagtcgtaga cgaagtacat gttggtgccg ttctggtagc tgtgagagga gtgaccgccc
    71221 agcatggtga tgtcatcggc gtacgggaac tcggtacgaa tacggttaat aacgctttcg
    71281 tagatttcgt gaatgcagct ccagcagccg gacacttcag tggtaaagcc catgttgccg
    71341 gttttgagga tctgcacacg ttcggcagct actttatccg gtccccagtt caggttgtta
    71401 aaccaggttt cgatcagctt gctgtccacg cgctggcatt gcgggtaacg tgccacgatt
    71461 tcagcaatcc cttcgcccgt tgccttcgcc atgcgaggat tcccctcagc cataaagatc
    71521 agcacgcatt tgccgtcggc aaaatgggtg aagtgttggg tgccatcttc agcatcatac
    71581 aaacgagcaa tcgacggacg atacccttcc accatgattt cacgcaggat gttgaagccg
    71641 gttttcatgt cttccagcac atagccatag aagagattgt tttccggcgt aaatttgaag
    71701 attttcactg tgacttcagt gatatagcac agtgcacctt cgttgccgat gatgatatga
    71761 cgaatgtccg ggccagccgc gcgacgtggc acgtttttaa tgcgtgtgac ggtgccatct
    71821 gccagcaccg cttccagacc aacgaccata tcctcgattg cgccgtagag tgtggaaaac
    71881 tgcccgatac tgcgggtcgc caccaggccg cccatctgcg ccagcggctt tgactgcgga
    71941 gaatgccccg tggtgtaacc tttttcacgc aacgcgtttt ccagcacctc cagcggaacg
    72001 ccacattgcg ccgtggcctg catattctca atatcaatat taatgatttg attcatggcg
    72061 gagccgtcga gcaccaccga gttttccaca acggtttcca gcccaccttc ggtagcggaa
    72121 gcaccggtac gcggcacacc gttaattttg tgcgcattca taaaattcag cacacgggat
    72181 acttgttctg tggaaccgag ttttacgacc gctgccggaa tcggcaaagt ataaatgcca
    72241 tgaatatccg gaaatttacg aaaacggtca atactgtttt tctttaatac tgtttcatcg
    72301 gtaattacgc gatctgcacc aacaatttcc tttagctggt cgacaatcgc tgcgcgagat
    72361 aaagacatag taattccttc ctgataataa aaatggaata atcattaata agagaaaaca
    72421 gaaaccaccg ttatggatta gcgcactaaa taaccgccat caaccactaa taaatgtccg
    72481 ttgacataat tcgatgccgg acttgcgagg aatacggctg cgcccattaa atcctgagta
    72541 tcgccccaac ggtttgccgg aatatgatca agaacgcgct ggttagtttc tggattacta
    72601 cgggtcgcca gagtgatatc ggtagcgtaa tatcccgggg caataccatt cacctgaata
    72661 ttatattgac ctaattcatc gcaataggct ttggtgaatc cggcaagagc atgtttagtg
    72721 gcagaataag ccggtgacca ttgaccacct aagtaagaga acaatgaaca gatattaatg
    72781 attttaccgc ttttctgcgg gatcataatt tttgcagctt catagcttaa ctcgaatgcg
    72841 gcggtaaggt tcacgtcaat catcggatcc cagtcggcac gcccgaagtc cagcacctta
    72901 ttcagcttac atataccggc attgttaacc agaatatcga cggtaccgaa acgctcacag
    72961 caggcagcga taatcttctg cggtgcgcct tctgcggtga tatccacctg catgaagtcc
    73021 acttcaacac cctgtttttc aatcatttcc tttgtttcgc cgttatcttt gacgaaactt
    73081 ggaataaaga catttgcgcc agccttggcc agcgccatgg caaatgcctg gcctaatccg
    73141 ctattcccgc cggtaacaat tgctgtttta cctttcaggg agaaaaaatc cattgagaac
    73201 gcattgagag attcgattga catagttagc tccaatttcc gtaaggcaaa aaaaaagaaa
    73261 ggtaacttcc cccgcgagag ggaagttacc tttctcatta tctctggtaa ctggagataa
    73321 tttttagcat aagggcataa ctgctaatgt gatcgtaatc acagagtgat gttcgtatta
    73381 tcaaatagtt ttaaaaagca gaagcgtgat tactctcaca caacaccttt tctttccatg
    73441 ttatttctta ttgcaattac tagagacctg agaaaagtaa tccacctgaa agggcggctt
    73501 acttttctct tttttttgtc tattacaaat gggaatatga tatgcaacac aactcatatc
    73561 gccgttggat aaccctcgcg ataattagtt ttagcggcgg cgttagtttc gacctggctt
    73621 atttacgtta tatttatcaa attcccatgg caaaatttat gggattcagc aataccgaga
    73681 taggtttaat aatgagtacc tttggtattg cggccattat tttctatgcc cctagcggcg
    73741 ttattgccga taaattttca caccgcaaaa tgattacttc cgcgatgatc attaccggat
    73801 tactgggcct gataatggca acatatccac cactgtgggt aatgctctgt attcaggtcg
    73861 cctttgcgat aacgacgatt ttaatgctgt ggtcggtgtc gattaaagcc gcatcgttgc
    73921 tgggcgatca tagcgagcaa gggaaaatta tgggctggat ggaagggctg cgcggcgtcg
    73981 gtgtaatgtc gctggcggtg tttaccatgt gggtcttttc tcgctttgca ccggatgaca
    74041 gcgccagcct gaaaacggtc attattatct atagtgtggt ttacattctg ctgggcattt
    74101 tgtgttggtt ttttgttagc gataacaaca atctgcgcag tgccaataac gaagaaaaac
    74161 agtcattcca gcttagcgac atcctggccg ttttgcgcat cagcaccacc tggtattgca
    74221 gcatggtgat ttttggtgtc ttcaccatct acgccattct gagttactcc accaactatc
    74281 tgaccgaaat gtacggcatg tcactggtgg cagcgagcta catggggatt gtgatcaaca
    74341 aaatcttccg cgcgttatgc ggcccacttg gcggcattat caccacctac agcaaagtga
    74401 aatcccctac ccgcgtggtc caaatccttt ccatcatcgg tttgctggca ttgacagccc
    74461 tgctcgtcac gaactctaac ccgcaatcgg tcgcgatggg gattggcttg attttattac
    74521 tgggattcac ctgttacgcc tcacgcgggc tgtattgggc ctgccctggc gaagcgagaa
    74581 caccgtctta cattatgggc accacggtag gtatttgttc ggtgattgga ttcctgccgg
    74641 atgtcttcgt ttacccgatt atcggctact ggcaagacac cctgcccgct gcagaagcct
    74701 accgcaacat gtggctaatg ggcatggcgg cgcttggcat ggtgatcatc tttacctttt
    74761 tgctgttcca aaaaattcgt actgctgata gcgcccccgc aatggctagc agcaagtaag
    74821 ccaggaaaat tacaggagaa aaatcatgtc gaaaaaatac atcataggga ttgatggcgg
    74881 aagtcagagc acaaaagtgg tgatgtacga tctggaaggt aatgtggttt gcgaaggcaa
    74941 aggcttattg cagccgatgc acacgccaga tgccgatacc gcagaacatc ctgacgacga
    75001 tttatgggca tcactatgtt ttgccagtca cgatttgatg agccagtttg ccggaaataa
    75061 agaagatatt gtcggcattg gcctgggctc aatccgttgc tgccgtgcgt tattgaaagc
    75121 cgatggtacg ccagccgcgc cgttgattag ctggcaggat gcacgcgtta cacgccctta
    75181 cgaacacact aatcctgacg tggcctatgt cacctctttt tcgggttatc tgacacatcg
    75241 cttaaccggc gagtttaaag acaatatcgc caactatttt ggtcagtggc cggtggatta
    75301 taagagctgg gcatggagcg aaaatgccgc ggtaatggaa aagtttaata tcccccgcca
    75361 gatgcttttt gacgtgcaaa tgccgggcac tgttcttggg catatcacac cacaagctgc
    75421 gctggcgaca cattttccgg ctgggctgcc ggttgtttgt accaccagtg ataaaccggt
    75481 agaagctctg ggagccgggt tactggatga tgaaacggcg gtaatttctc ttggcaccta
    75541 tatcgcgttg atgatgaacg gcaaagcact gccgaaagat ccggtcgcgt actggccgat
    75601 tatgtcttct attccgcaaa cattgctgta tgaaggttac ggtattcgca aaggcatgtg
    75661 gacggtgagc tggctgcgcg acatgttagg cgagtcgtta attcaggatg ccaaagcaca
    75721 ggatctttca ccggaagatt tgctcaacaa aaaagcgtct tgtgtgccgc ctggctgtaa
    75781 tggtctgatg acggtgctgg actggctgac caatccgtgg gaaccgtaca aacgcgggat
    75841 tatgatcggt tttgattcca gcatggatta cgcatggata tatcgttcga tactggaaag
    75901 cgtggcgctg acgctgaaga ataattacga caatatgtgt catgaaatga atcactttgc
    75961 aaagcatgtg atcattactg gcggcggttc gaacagcgat ctgtttatgc agatttttgc
    76021 cgacgtgttc aaccttccgg cacgccgaaa cgccattaac ggttgtgcaa gtctgggggc
    76081 tgcgatcaat acggcggtag gtctggggct atacccggat tacacaacgg ctgtcgataa
    76141 aatggttcgc gtgaaagata tcttcatacc gattgagagc aatgccaaac gctacgacgc
    76201 gatgaataaa ggcattttca aagacctaac caaacatact gatgtgatcc tgaaaaaatc
    76261 gtatgaagtg atgcatgggg aattggggaa tgcggattcg atccagagct ggtcgaatgc
    76321 gtaagtgagg aaggccgggc gggaaactgt ccggcctgaa catacctgaa tggttatccc
    76381 cgctgacgcg gggaactcat atccggtttt tgctggtggg ccgcgccgtt cggtttatcc
    76441 ccgctggtgc ggggaactct aaacataacc tattattaat taattatttt ttaagacagt
    76501 cacaatctac caactttaca gtatcacaca aacaacacat ccattatgtt aaagagcact
    76561 taatccattg attaaaaaga taaatattta caatgactct attcaaacta aaatctacca
    76621 aactttaccg caataatttt cactccagcg aaaaattaac gccacagaat ttgtagagaa
    76681 tgataatgat tagtccagaa tgggcaaaat ggaggttttt cagagacgac aaagaatata
    76741 aatgagtaga agtctttaca gattccaccc att
//
LOCUS       DAZGJT010000006        75285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZGJT010000006 DAZGJT010000000
VERSION     DAZGJT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 75285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.006"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..701)
                     /locus_tag="ACHKFK_000436"
     CDS             complement(<1..701)
                     /locus_tag="ACHKFK_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9050862.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGGVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGYYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLPAEKIRSLYRESDIIPGEQTATSILKQR
                     TKNIALPLHAHQQQIR"
     gene            complement(703..981)
                     /locus_tag="ACHKFK_000437"
     CDS             complement(703..981)
                     /locus_tag="ACHKFK_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050863.1"
                     /translation="MAHDTKLHNSNDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(1149..1304)
                     /gene="hokD"
                     /locus_tag="ACHKFK_000438"
     CDS             complement(1149..1304)
                     /gene="hokD"
                     /locus_tag="ACHKFK_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9050864.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1542..2207)
                     /locus_tag="ACHKFK_000439"
     CDS             complement(1542..2207)
                     /locus_tag="ACHKFK_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFO9050865.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(2382..2807)
                     /locus_tag="ACHKFK_000440"
     CDS             complement(2382..2807)
                     /locus_tag="ACHKFK_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9050866.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGCGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICCECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(2848..3930)
                     /locus_tag="ACHKFK_000441"
     CDS             complement(2848..3930)
                     /locus_tag="ACHKFK_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO9050867.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNLGQPAGTTPEADSAYALKADSGAVQQVMTARPEQ
                     SHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAF
                     SAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLP
                     VSGTNNQRCVNHISEPDNEIPPGFRG"
     gene            complement(4001..4426)
                     /locus_tag="ACHKFK_000442"
     CDS             complement(4001..4426)
                     /locus_tag="ACHKFK_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9050868.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(4410..4652)
                     /locus_tag="ACHKFK_000443"
     CDS             complement(4410..4652)
                     /locus_tag="ACHKFK_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9050869.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            5044..5382
                     /locus_tag="ACHKFK_000444"
     CDS             5044..5382
                     /locus_tag="ACHKFK_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9050870.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            5671..5826
                     /locus_tag="ACHKFK_000445"
     CDS             5671..5826
                     /locus_tag="ACHKFK_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9050871.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            5828..5956
                     /locus_tag="ACHKFK_000446"
     CDS             5828..5956
                     /locus_tag="ACHKFK_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050872.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLFSSAKSNG"
     gene            5986..6204
                     /gene="ydfC"
                     /locus_tag="ACHKFK_000447"
     CDS             5986..6204
                     /gene="ydfC"
                     /locus_tag="ACHKFK_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9050873.1"
                     /translation="MQKREPVIIAPDYTDNELYEWMRQKINAAQDLKWANEARAKQAE
                     NLSALEQDITRLEKAAALSIARMITYPR"
     gene            6773..6961
                     /gene="dicB"
                     /locus_tag="ACHKFK_000448"
     CDS             6773..6961
                     /gene="dicB"
                     /locus_tag="ACHKFK_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9050874.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            6958..7146
                     /locus_tag="ACHKFK_000449"
     CDS             6958..7146
                     /locus_tag="ACHKFK_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9050875.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            7242..9626
                     /locus_tag="ACHKFK_000450"
                     /pseudo
     CDS             7242..9626
                     /locus_tag="ACHKFK_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
     gene            9691..9939
                     /locus_tag="ACHKFK_000451"
     CDS             9691..9939
                     /locus_tag="ACHKFK_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO9050876.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPGTLSKYAKAGMIFPLPKKVGRH
                     WRVDPRARFVGMVNKPEVIATDHPALKRILEDGAPTKI"
     gene            9917..11047
                     /locus_tag="ACHKFK_000452"
     CDS             9917..11047
                     /locus_tag="ACHKFK_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO9050877.1"
                     /translation="MARPRKYKTEVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKQAEAIAAEANSRLARQQMEQMLTLQEKIIRDTGGSSTVSVFLNSYRKIQQERYENG
                     EIKLNTLKQKASPLRVFDERFGTRPLDAITVKDVVSVLEDYKAKGHNRMGQIFRKVLI
                     DVFREAQQTGDVSPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMKFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGIDTQILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(11093..11731)
                     /gene="ompW"
                     /locus_tag="ACHKFK_000453"
     CDS             complement(11093..11731)
                     /gene="ompW"
                     /locus_tag="ACHKFK_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO9050878.1"
                     /translation="MKKLTVAALAVATLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            11969..12058
                     /locus_tag="ACHKFK_000454"
                     /pseudo
     CDS             11969..12058
                     /locus_tag="ACHKFK_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            12086..12829
                     /gene="yciC"
                     /locus_tag="ACHKFK_000455"
     CDS             12086..12829
                     /gene="yciC"
                     /locus_tag="ACHKFK_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO9050879.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGVFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            12859..13398
                     /gene="yciB"
                     /locus_tag="ACHKFK_000456"
     CDS             12859..13398
                     /gene="yciB"
                     /locus_tag="ACHKFK_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO9050880.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQSVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLNGIYIYRHMPQEDKS"
     gene            13504..13902
                     /gene="yciA"
                     /locus_tag="ACHKFK_000457"
     CDS             13504..13902
                     /gene="yciA"
                     /locus_tag="ACHKFK_000457"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO9050881.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(13942..14658)
                     /gene="tonB"
                     /locus_tag="ACHKFK_000458"
     CDS             complement(13942..14658)
                     /gene="tonB"
                     /locus_tag="ACHKFK_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO9050882.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQP
                     QYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPGK
                     PGSGIVVNILFKINGTTEIQ"
     gene            14882..15178
                     /gene="yciI"
                     /locus_tag="ACHKFK_000459"
     CDS             14882..15178
                     /gene="yciI"
                     /locus_tag="ACHKFK_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO9050883.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            15478..16731
                     /gene="kch"
                     /locus_tag="ACHKFK_000460"
     CDS             15478..16731
                     /gene="kch"
                     /locus_tag="ACHKFK_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO9050884.1"
                     /translation="MSHWTTFKQTATKLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(16786..16959)
                     /gene="yciY"
                     /locus_tag="ACHKFK_000461"
     CDS             complement(16786..16959)
                     /gene="yciY"
                     /locus_tag="ACHKFK_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO9050885.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            17102..18562
                     /gene="cls"
                     /locus_tag="ACHKFK_000462"
     CDS             17102..18562
                     /gene="cls"
                     /locus_tag="ACHKFK_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO9050886.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            18597..18926
                     /gene="yciU"
                     /locus_tag="ACHKFK_000463"
     CDS             18597..18926
                     /gene="yciU"
                     /locus_tag="ACHKFK_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO9050887.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(18979..19983)
                     /gene="oppF"
                     /locus_tag="ACHKFK_000464"
     CDS             complement(18979..19983)
                     /gene="oppF"
                     /locus_tag="ACHKFK_000464"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO9050888.1"
                     /translation="MNAVTEGRKVLLEIAALKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(19980..20993)
                     /gene="oppD"
                     /locus_tag="ACHKFK_000465"
     CDS             complement(19980..20993)
                     /gene="oppD"
                     /locus_tag="ACHKFK_000465"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO9050889.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(21005..21913)
                     /gene="oppC"
                     /locus_tag="ACHKFK_000466"
     CDS             complement(21005..21913)
                     /gene="oppC"
                     /locus_tag="ACHKFK_000466"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO9050890.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(21928..22848)
                     /gene="oppB"
                     /locus_tag="ACHKFK_000467"
     CDS             complement(21928..22848)
                     /gene="oppB"
                     /locus_tag="ACHKFK_000467"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO9050891.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(22934..24565)
                     /gene="oppA"
                     /locus_tag="ACHKFK_000468"
     CDS             complement(22934..24565)
                     /gene="oppA"
                     /locus_tag="ACHKFK_000468"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO9050892.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            complement(25121..25276)
                     /locus_tag="ACHKFK_000469"
     CDS             complement(25121..25276)
                     /locus_tag="ACHKFK_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050893.1"
                     /translation="MLRLLRHYFYFVPAQLSKAKGIGKNILLKLQLVIGISLTASPHH
                     HVIFATS"
     gene            complement(25303..25950)
                     /gene="ychE"
                     /locus_tag="ACHKFK_000470"
     CDS             complement(25303..25950)
                     /gene="ychE"
                     /locus_tag="ACHKFK_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO9050894.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(25981..26034)
                     /gene="ychE"
                     /locus_tag="ACHKFK_000471"
     CDS             complement(25981..26034)
                     /gene="ychE"
                     /locus_tag="ACHKFK_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO9050895.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            26427..29102
                     /gene="adhE"
                     /locus_tag="ACHKFK_000472"
                     /gene_synonym="adhC"
     CDS             26427..29102
                     /gene="adhE"
                     /locus_tag="ACHKFK_000472"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO9050896.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDTTGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            29228..30259
                     /locus_tag="ACHKFK_000473"
                     /pseudo
     CDS             29228..30259
                     /locus_tag="ACHKFK_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418698.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(30544..31161)
                     /gene="tdk"
                     /locus_tag="ACHKFK_000474"
     CDS             complement(30544..31161)
                     /gene="tdk"
                     /locus_tag="ACHKFK_000474"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO9050897.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALEVGSLTAIQERHRHD"
     gene            31765..32178
                     /gene="hns"
                     /locus_tag="ACHKFK_000475"
     CDS             31765..32178
                     /gene="hns"
                     /locus_tag="ACHKFK_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO9050898.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(32322..33230)
                     /gene="galU"
                     /locus_tag="ACHKFK_000476"
     CDS             complement(32322..33230)
                     /gene="galU"
                     /locus_tag="ACHKFK_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO9050899.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(33432..34445)
                     /gene="rssB"
                     /locus_tag="ACHKFK_000477"
     CDS             complement(33432..34445)
                     /gene="rssB"
                     /locus_tag="ACHKFK_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO9050900.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(34537..35442)
                     /gene="rssA"
                     /locus_tag="ACHKFK_000478"
     CDS             complement(34537..35442)
                     /gene="rssA"
                     /locus_tag="ACHKFK_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO9050901.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPFVRTNI"
     gene            35555..36013
                     /gene="ychJ"
                     /locus_tag="ACHKFK_000479"
     CDS             35555..36013
                     /gene="ychJ"
                     /locus_tag="ACHKFK_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO9050902.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGATALRAELIAGFAHTEWLGLTVFEHCWQDGGNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            36063..36905
                     /gene="purU"
                     /locus_tag="ACHKFK_000480"
     CDS             36063..36905
                     /gene="purU"
                     /locus_tag="ACHKFK_000480"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO9050903.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            37065..37149
                     /locus_tag="ACHKFK_000481"
     tRNA            37065..37149
                     /locus_tag="ACHKFK_000481"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37099..37101,aa:Tyr,seq:gta)
     gene            37196..37327
                     /locus_tag="ACHKFK_000482"
     ncRNA           37196..37327
                     /ncRNA_class="other"
                     /locus_tag="ACHKFK_000482"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            37581..38780
                     /locus_tag="ACHKFK_000483"
     CDS             37581..38780
                     /locus_tag="ACHKFK_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO9050904.1"
                     /translation="MPARQVCQNFFRGALAPFHKYRQNALLDATIALINGASLTLTSI
                     GRYLPGNAQVKNKIKRVDRLLGNESLHHDIPLIFRNIISMLTSKLSLCVIAVDWSGYP
                     SQEYHVLRASLICDGHSIPLLSWIVPSEKQQNAKIQQAFLNTLSEAVNPKARVIIVTD
                     AGFQNAWFRHIKSLGWDFIGRIRGNKQLHLARKGECWFRRQELQAGNKPEYLGPGTLS
                     RAEYARCDGHFYLHKKEPKGRRNKRSRCGIARPSQIKDARSAAKEPWLIFSSTDDFKP
                     REIMKLYSRRMQIEQNSRDEKSERFGFGLRASYSCSAGRMLVLSLLATLSTIVLWLIG
                     YHAENQGLHLRYQANSIKTRRVISYLRLAENVLRHSPLILKRTVLSTILNHLTRTYQN
                     MVLVYYR"
     gene            complement(38833..39510)
                     /gene="narI"
                     /locus_tag="ACHKFK_000484"
     CDS             complement(38833..39510)
                     /gene="narI"
                     /locus_tag="ACHKFK_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9050905.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(39510..40220)
                     /gene="narJ"
                     /locus_tag="ACHKFK_000485"
     CDS             complement(39510..40220)
                     /gene="narJ"
                     /locus_tag="ACHKFK_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9050906.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLPKEDAHALG
                     IFLRDLTTMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQIKFFAEKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(40217..41755)
                     /gene="narH"
                     /locus_tag="ACHKFK_000486"
     CDS             complement(40217..41755)
                     /gene="narH"
                     /locus_tag="ACHKFK_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9050907.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTICSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(41752..45495)
                     /gene="narG"
                     /locus_tag="ACHKFK_000487"
     CDS             complement(41752..45495)
                     /gene="narG"
                     /locus_tag="ACHKFK_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO9050908.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAREAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSSKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKELGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(45888..47279)
                     /gene="narK"
                     /locus_tag="ACHKFK_000488"
     CDS             complement(45888..47279)
                     /gene="narK"
                     /locus_tag="ACHKFK_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO9050909.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPLLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            47618..49414
                     /gene="narX"
                     /locus_tag="ACHKFK_000489"
     CDS             47618..49414
                     /gene="narX"
                     /locus_tag="ACHKFK_000489"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO9050910.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANTSGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            49407..50057
                     /gene="narL"
                     /locus_tag="ACHKFK_000490"
     CDS             49407..50057
                     /gene="narL"
                     /locus_tag="ACHKFK_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO9050911.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(50058..51452)
                     /gene="ychO"
                     /locus_tag="ACHKFK_000491"
                     /gene_synonym="iatA"
     CDS             complement(50058..51452)
                     /gene="ychO"
                     /locus_tag="ACHKFK_000491"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO9050912.1"
                     /translation="MSRFIPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     DVGQRWARGSWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSEEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            51637..51990
                     /gene="ychN"
                     /locus_tag="ACHKFK_000492"
     CDS             51637..51990
                     /gene="ychN"
                     /locus_tag="ACHKFK_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO9050913.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQENNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(52034..52729)
                     /gene="chaC"
                     /locus_tag="ACHKFK_000493"
     CDS             complement(52034..52729)
                     /gene="chaC"
                     /locus_tag="ACHKFK_000493"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO9050914.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAQQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNELLVSVKKLLA
                     ENYPDGVLRPGLA"
     gene            complement(52887..53117)
                     /gene="chaB"
                     /locus_tag="ACHKFK_000494"
     CDS             complement(52887..53117)
                     /gene="chaB"
                     /locus_tag="ACHKFK_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO9050915.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDWRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            53387..54487
                     /gene="chaA"
                     /locus_tag="ACHKFK_000495"
     CDS             53387..54487
                     /gene="chaA"
                     /locus_tag="ACHKFK_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO9050916.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSLWHAIWLIVHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            54893..55000
                     /locus_tag="ACHKFK_000496"
     CDS             54893..55000
                     /locus_tag="ACHKFK_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9050917.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(55148..56002)
                     /gene="kdsA"
                     /locus_tag="ACHKFK_000497"
     CDS             complement(55148..56002)
                     /gene="kdsA"
                     /locus_tag="ACHKFK_000497"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO9050918.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(56038..56847)
                     /gene="sirB1"
                     /locus_tag="ACHKFK_000498"
     CDS             complement(56038..56847)
                     /gene="sirB1"
                     /locus_tag="ACHKFK_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO9050919.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGEWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(56851..57243)
                     /gene="sirB2"
                     /locus_tag="ACHKFK_000499"
     CDS             complement(56851..57243)
                     /gene="sirB2"
                     /locus_tag="ACHKFK_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO9050920.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTLLLLSGIALMAKAHIQPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(57240..58073)
                     /gene="prmC"
                     /locus_tag="ACHKFK_000500"
     CDS             complement(57240..58073)
                     /gene="prmC"
                     /locus_tag="ACHKFK_000500"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9050921.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFIFAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(58073..59155)
                     /gene="prfA"
                     /locus_tag="ACHKFK_000501"
     CDS             complement(58073..59155)
                     /gene="prfA"
                     /locus_tag="ACHKFK_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO9050922.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(59197..60453)
                     /gene="hemA"
                     /locus_tag="ACHKFK_000502"
     CDS             complement(59197..60453)
                     /gene="hemA"
                     /locus_tag="ACHKFK_000502"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO9050923.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEERDDLQEALIRWLCDYHNLNEDDLRNSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLFTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSDIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQEASEFMA
                     WLRAQSASETIRDYRSQAEQVRDELTAKALAALEQGGDAQTIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            60667..61290
                     /gene="lolB"
                     /locus_tag="ACHKFK_000503"
     CDS             60667..61290
                     /gene="lolB"
                     /locus_tag="ACHKFK_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO9050924.1"
                     /translation="MPLPDFRFIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            61290..62141
                     /gene="ispE"
                     /locus_tag="ACHKFK_000504"
     CDS             61290..62141
                     /gene="ispE"
                     /locus_tag="ACHKFK_000504"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO9050925.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLSTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            62292..63239
                     /gene="prs"
                     /locus_tag="ACHKFK_000505"
     CDS             62292..63239
                     /gene="prs"
                     /locus_tag="ACHKFK_000505"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO9050926.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            63364..65043
                     /gene="dauA"
                     /locus_tag="ACHKFK_000506"
     CDS             63364..65043
                     /gene="dauA"
                     /locus_tag="ACHKFK_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO9050927.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNLEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(65098..65376)
                     /gene="ychH"
                     /locus_tag="ACHKFK_000507"
     CDS             complement(65098..65376)
                     /gene="ychH"
                     /locus_tag="ACHKFK_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO9050928.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            65654..66238
                     /gene="pth"
                     /locus_tag="ACHKFK_000508"
     CDS             65654..66238
                     /gene="pth"
                     /locus_tag="ACHKFK_000508"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO9050929.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            66355..67446
                     /gene="ychF"
                     /locus_tag="ACHKFK_000509"
     CDS             66355..67446
                     /gene="ychF"
                     /locus_tag="ACHKFK_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO9050930.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAVRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            67638..67814
                     /locus_tag="ACHKFK_000510"
     CDS             67638..67814
                     /locus_tag="ACHKFK_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9050931.1"
                     /translation="MKKTFTFSLLAIVFASLLSACVPHHHHRHSDGPRGPAPSGKPMP
                     PSNGGPNHGSHGRY"
     gene            complement(67851..69770)
                     /gene="dhaR"
                     /locus_tag="ACHKFK_000511"
     CDS             complement(67851..69770)
                     /gene="dhaR"
                     /locus_tag="ACHKFK_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001467850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO9050932.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSTLGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRG"
     gene            69998..71068
                     /gene="dhaK"
                     /locus_tag="ACHKFK_000512"
     CDS             69998..71068
                     /gene="dhaK"
                     /locus_tag="ACHKFK_000512"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO9050933.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     SEPGIDRRPFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLAFWDAPVHTPALNWGK"
     gene            71079..71711
                     /gene="dhaL"
                     /locus_tag="ACHKFK_000513"
     CDS             71079..71711
                     /gene="dhaL"
                     /locus_tag="ACHKFK_000513"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO9050934.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQTTQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSCEQNLPVPAALDAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            71722..73140
                     /gene="dhaM"
                     /locus_tag="ACHKFK_000514"
     CDS             71722..73140
                     /gene="dhaM"
                     /locus_tag="ACHKFK_000514"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO9050935.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDNCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLSV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAETSGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPLSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            73460..75157
                     /gene="treA"
                     /locus_tag="ACHKFK_000515"
     CDS             73460..75157
                     /gene="treA"
                     /locus_tag="ACHKFK_000515"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9050936.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETSVTPQPPDILL
                     GPLFNDVQNAKLFPDQKIFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPKPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILSRASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRASKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPLSESTTQPLKQKEAEPTP"
BASE COUNT        19133 a        19181 c        18212 g        18759 t
ORIGIN      
        1 cggatttgtt gctggtgggc gtgaagtggt agcgcaatat tttttgtgcg ctgcttcagt
       61 atgctggtgg ctgtctgctc tcccggtatg atgtcgctct cgcgataaag cgagcggatt
      121 ttctccgccg gtaatcccag tgagcggcgt aatacagcct ccggtagtgc gtccgctacc
      181 tgattgcaga ccgcccacca ggataattca gccagagata attcacgctc ctgcgtaccg
      241 cttattgcgt gaccgatgac gtcaatcatc catgctgaca ggttttgatg agcaagttgc
      301 tcgagtgatt cggatgtctg gtcacgcagc tggttgtcgc agtgccagca caacaccatt
      361 gcgccggtac cataacggtg aatgacggtt tcgctgtgat gataatagcc gtgtggccac
      421 tggcaggatt taacatggcg cagtaaccag tcagacaatg cgccagcgcc accagcagca
      481 cgaatcactc gttcgtcgct gaaaaatggc agtaatgatt tatcctccgc cagcggctgg
      541 cgaacggcag gaacgccccc ggacggcaga ttacgcatgc ttttcggttc cggctccacc
      601 agtacccggg tattgtggaa taccggcatg gattcacggc ccggcttaac gatcaccagc
      661 ccgagttccg gtaccagaac aggtcgaagt aatacccgca cgttacctcc agatgcgttg
      721 ctggaatgtg cgggacggac gcggtgggcg ctcggagtac ggcagcctga cgtagattat
      781 ccagtgacga taatcgaggc tgagggcttt cttaacctcg tatccacgcc tgcggtaaca
      841 ctgaattatc cattcagcct gctcttcagt gcatggagga tgctggaacc agtctgattt
      901 gaatgcgtga aaacgccgtc cgcacctgct ggcaaagacg gcagaatcat tagaattgtg
      961 taatttggta tcgtgcgcca tcggttgtct ctgctggcgc agcaggtgcc agttgttcag
     1021 gctggcgtgc gaattgtaaa ccagaatgcc aggaaaaaac aaaacccgcc gaagcgggtt
     1081 aagtgcgggt gcgttgagga tgcctgacac atcagaggtg gcgagggatt tctcccccgc
     1141 ctggtctctt actcctcagg ttcgtaagct gtgaagacag cgacctccgt ctggccggtt
     1201 cggattcgta cctcgcagag gtctttcctc gttaccagtg ccgtcactat gacggttaaa
     1261 cagatgacga tcagggcgat taacatcgcc ttttgctgct tcatagcctg cttctccttg
     1321 cctttcggca cgtaagaggc taacctacat gtgttcagca tggattgagc ctcagattaa
     1381 tgttaagcgt cttgcagaac gcgtaatgtt aactggggct tttctctatc tgccttttgg
     1441 tgttcatgcc tgaggcagat agcctcaagc acccgcagca attctactta actcacgtca
     1501 cctcgccaat atgaaatcaa tcagaaaggt gatccataaa atcactcctt ctcttctttt
     1561 ccgtagtgga gttggccaat tttgataaga gggcgtccct gagatttgcg gtgtagattg
     1621 gtatcgcgca gagaatacac acagccacaa tattcctgct gatagaattt ttcgcgcttg
     1681 ctgatttcaa tcatacggga cgagccgccc tgcttgcgcc agttataatc ccagtatacc
     1741 atacccggat aatgcgcaac agctcgccgc ccacactcgt taacctgctg catatttttc
     1801 cagcgcgaaa tgcccagtga actgctgatc acactgaaac cattttcagc agcgtacaac
     1861 gctgtccgct caaaacgcat gtcaaaacac atggtacaac ggatccccct ctcaggctcc
     1921 cattccattc ctttggcacg ttcaaaccag ttgtcggtgt cgtaatcagc atcgataaac
     1981 ggcacgccgt gttgttcagc aaagcgaata ttttcatcct tacgaattaa atactctttc
     2041 tgaggatgaa tgttcgggtt gtagaaaaag atggtgtagt cgattcccga ggcctgaagc
     2101 gcctccatca cttcaccgga acatggagca cagcaagagt gcagtagtag tttgtttgcc
     2161 ccgtttggga gctccaattt aggccgtttg aaatcagcaa tagtcataaa tatttttatt
     2221 ggggtcatga aaatagcaca gagtgtagca tcagagcagg gctatcggga atatatgtct
     2281 aaatctggta atatctggtt ttgacgcaaa gcggacaacc acgctggctc taccctgcgc
     2341 catgaaaatg tcaattcaca tctgaactaa tgctctttaa tctagtaacg tctaaaatac
     2401 ctaacatttc cttgataaaa tgccagtaca cgctgcatag cttcgctctt ccggcactcg
     2461 caacagatta tattcagacg cctgtcgtag cggcgtattt cgccgtctgg taacgaccag
     2521 ataaggtccg gatcaaccac tgcaggtttc ttcacctttg cccttgagag ttttttgcgg
     2581 gcattttgcc agtccttacg cgcctgttca gacgggaata atccgcagcc ggagttgtat
     2641 acatcgccac tggctaccag ttctctggca agaacgctca tcagatatct tgttgcccca
     2701 gttttagctt ccagttgtcg taacgtctcg cgcccactct ggcgtacgag ttcaacaacc
     2761 tgccctttaa ttttttcccg ctcttcttgt gtaaaaactt ttgccacaag ccctcctgaa
     2821 aattacctca tgaccagaaa ttaacactta ccccctgaag cccggcggaa tttcgttatc
     2881 cggttcagaa atatgattca cacaacgctg gttgttcgtg ccgcttaccg ggagcaacca
     2941 ggggttctca aaattccggt ccggtccaaa aaacgtcgtc gctcgctgaa caaattccgt
     3001 tcccgttttc ccggtagccg ccaagtatct tgcgtaacgc ctcacgccat ccagcatggc
     3061 ttctggtggc accccctcgc gtaatctggc cttccaggca ctgaaagcgg atttcttcgg
     3121 gtttgctccg gcacgcaacg ggtactcccg ccagacctgt tcgaacacat ccggataatc
     3181 cactcgtccc acagactgcc cggtgttttc cgggactacc cgatcggctt cccgctgaat
     3241 ggcggaatcg gcttcgggct gctgcagttg gtgtgattgc tccggccttg cggtcatcac
     3301 ctgctgcaca gcgcccgaat cggctttcag cgcatacgct gaatcggctt ccggtgtcgt
     3361 gcctgctggc tgaccaagat tgacggtctg aacatcccct gcctggttcg tggcgttttt
     3421 tacgccatgg accatagtgt tttgatcttc ttgatctgta tctttatctg tatctttatc
     3481 tgtatcttta tctgtcgtga ctcgtcgtga catgtgcgtg acatttcgtg acgcgccgtg
     3541 acaatcgcca ttttgttccc gctttctttc cctctctcgc tgcgccctct tgcgctctgc
     3601 aggagatttt gcggtttgcg aaatattgcc gttgtcctct ttaagcacct ggcgtttttc
     3661 ccatccagtg attaaatcac catcaagtac ccgcccctgc atcgtctgca aaattgaatc
     3721 aattacctct tctgtcacgt cgagcgcact tgccaaatct tctgtcgtga catcaatgtg
     3781 acctcgcgtg acatttcgtg acgcgctcac caggaggtgg atatacactg ccatcactgt
     3841 tgcaattggc tgccctgaca ctctggcaat tgttcgccac ttagggtcat ttggcatgtc
     3901 atgccataat ctgagccagg cgttagccat actcacctct tctgataccg aatcttttac
     3961 tcacgagttg ccggaagcga ttcgatatgg ctattatcac tcaatgcact gccacagcat
     4021 ttcctgccgg gccaccacgg ttcatctgat tgaaaccggc gattgccact gcgacaaaat
     4081 catcagcgtc tctcaccagt cgctcccgcg tctccactag ttcccgaaaa taggctgagc
     4141 tatgactgcg cattcgggcc accagcagag gtggcattgc tttttcgata gctggtaaca
     4201 acgcctgaat tttttcaact gcatcagggg tgtctttctc tatccagcgg aaaattttct
     4261 gggtattgcg agccagggct tccggatggc tgtcgtcgta cagttcagga aacgtcatac
     4321 ccaactcaaa ataagcctgg gttattccag ctgccggaac tttttcgcca tcaggacgcg
     4381 cccaggcatt catcgccatg cggatgtgtt catgcttgat tttcatgaat ccccccttgg
     4441 ttagaaggcg gattatgatc agaaccggga atgacaaccg tcggtatgtg taactcatat
     4501 ttgagcgccc cggcagtgac tgcctgaatt agcaacgccc atttccacgg aacctcttcc
     4561 ccccacatgc tgactgtggt ttttgacgtt cctagagctg cggctgtttt aacaactccg
     4621 ccaaaatagc ctaatacttc tgattttttc atgagtcgct ccataaaact gaacgccaaa
     4681 agtttaataa tcaaaaccaa agaaagtcaa gaaacaaaac catctgtgtt ttaaaatcaa
     4741 aacatgagca agcaaacaat atctgaacgc ataacccaac gtatgcatgc gctaaacctg
     4801 aaaggcaaag accttgtcaa tgccactggc gcatcaaaag gctccgtaag tcaatggatg
     4861 aacggtggag gagcgccgtc ctcgcgttac ataagttcac tggcaaaaat attgaaagta
     4921 aacgaaaatt ggcttcttaa tggaggagag ttaaatacag gtgattcgct tgatctatct
     4981 ttaccgccga taaaaacggt tccgctacta tcacttcagc aggcagcaag ctggagtgat
     5041 tatatgaaaa attcctcaat aacctcttgt gtgcagcttg tcggagaaat cccggccaat
     5101 acctttgcag ttgttctaga gagtgacagt atgtcaacat ctggtggggg agtttccatc
     5161 ccaaatggtt caacagtttt tgttgatccc gatcgaaccg tacaaccagg aaatattgtc
     5221 cttgccttac ccaaagggac cacaacacct gtcattcgta aactggagat agaagggccg
     5281 gatattcttt tagtccccac gaatcctcgc tacccttcaa ttatgctgga tgatctatct
     5341 tgcatattgg gcgtatgctt taaaattcaa caagatattt aaccgacctc atctatttga
     5401 ttaactgtat gccatcgtgg tgatggctta acagctgcct gcttaaaatg ttttgataaa
     5461 aaaacattga cctgaaaagt tcatttttct aaacttcatt ccctctcccc accccacaga
     5521 atgcagggca atacttcgag ttaccaggca gtggtcaggg gttaagtagc cagcccgagg
     5581 cgtaagaaca tgacggcagg gttcaacttt aataactatg cagcaggttt ttgttccgct
     5641 accccggcgt taaggggaaa tgaggtcagc atggatacta tcgatcttgg caacagcgaa
     5701 tctctggtat gtggcgtgtt ccccaaccag gacggtacgt tcaccgcgat gacgtatacc
     5761 aaaagcaaaa cgtttaaaac cgaaaatggt gcccgtcgct ggctggaaag aaactcaggt
     5821 gagtgatatg gattttgaca caatcatgga aaaggcttac gaagaatact tcgaaggcct
     5881 tgccgaaggc gaagaagctc acagcttcaa cgaatttaaa caggtgcttt tcagttcggc
     5941 aaaatctaac ggctgataag cgaaacagca ccgcgaggaa tcagtatgca gaaacgagaa
     6001 cccgtcatca tcgctccaga ctataccgat aatgaacttt atgagtggat gcgccagaaa
     6061 attaatgcag cgcaggattt gaaatgggcc aatgaagcca gggctaagca ggctgaaaat
     6121 ctgtccgctc tggagcagga tatcaccagg ctggaaaaag cagcggcatt aagcattgcc
     6181 agaatgatta catacccgcg ttaatagcta accaacgaag ctaaggttgg taattaagta
     6241 gttctccacg ggtgaggtgg agtgcgtgcg ccggacgcgg gtgagcatcc ggcactgaca
     6301 gtttactgaa aggatatttc cctgaaaagt cagaccataa cgcgaaagcg cacggcaagg
     6361 tagctggttc atagatagcc tgtcgttaaa ttttcgtcga ccgtgcgctt ccggttgtgg
     6421 caacccgcga aatggtgcgg cggtaagtat ggcggggtta tttcttcccc cgttgaggac
     6481 accgggttgt caggttgacc atacgcttaa gtgacaaccc cgctgcaacg ccctctgtta
     6541 tcaattttct ggtgacgttt ggcggtatca gttttactcc gtgactgctc tgccgccctt
     6601 tttaaagtga attttgtgat gcggtgaatg cggctaagcg cacgcggaac agttaaaacc
     6661 aaaaacagtg ttatgggtgg attctctgta tccggcgtta attgttaact ggttaacgtc
     6721 acctggaggc accaggcact gcatcacaaa attcattgtt gaggacgcga taatggaaac
     6781 gttattacca aacgttaata cgtctgaagg ttgttttgat attggtgttc tgctcagtaa
     6841 ccgggagttt actgaagatg ccattaatat gaggaaatat gagccttatc tgctcaatga
     6901 taattccata ctttcccgaa ttgctcttct tgaacttggt attttcggag aacgtcaatg
     6961 acttcagcat ttgcactgat gatgacggtt tttcttataa cgggtgaatc acagaatgtg
     7021 attaccggaa tttatgccag taaagaatcc tgcctccagg caagagacga gcaaaaaatt
     7081 tctggtgaat gcctcccggt aaaaaaagta tcgctgtacc tgaataacga aacaccggct
     7141 ggataaccct ccagccatat taacaccata ccaacggatt aaaaatgcca gcaatggcag
     7201 ggattcgttc accctgaaat ctgtaatgag gttaaaacaa aatgagtaag gtctttattt
     7261 gcgctgctat tcctgatgaa caggccataa aagaagatag cgctgttgcg gtggccactg
     7321 ccattgaagc cggtgatgag cgtcgcgcac gcgcaaaatt tcattggcaa tttctggagc
     7381 aattccctgc agctcaggac tgcgcttata aatttattgt ctgtgaggat aaacccggca
     7441 taccccgccc tgccctcgat tcctgggatg ctgaatatat gcaggaaaac cgctgggatg
     7501 aggagtctgc ttcctttgtc ccggttgaga ctgaatctga tccgatgaac gtcacttttg
     7561 acaagctggc ccctgaagta cagaacgctg tcatggttaa gttcgacaca tgtgaaaaca
     7621 tcaccgttga tatggttatt agcgcacagg aattgttgca ggaagacatg gcaacattcg
     7681 acggacatat cgttgaagcg ttgatgaaaa tgccagaagt taacgccatg tatccggagc
     7741 ttaagctgca tgccatcggg tgggttaagc ataaatgtaa gcctggtgcc aaatggcccg
     7801 aaattcaggc agagatgcgc atctggaaaa aacgtcgcga aggtgaacgc aaggaaaccg
     7861 gaaaatacac gtctgttgtt gatctcgccc gcgccagaac caatcaacag cacactgaaa
     7921 attcaacagg aaaaatcagc ccggtcattg ctaccattca tcgcgaatac aagcagacat
     7981 ggaaaacact ggatgacgaa ctggcctacg ctctctggcc tggtgatgtg gatgccggaa
     8041 acattgacgg cagcatccat cgctgggcaa aaaatgaagt tatcgacaac gaccgcgaag
     8101 actggaagcg tatctcggca tcaatgcgca aacagcctga tgcccttcgc tacgaccgcc
     8161 agactatttt tggccttgtc cgtgaacgtc cgatcgacat tcacaaagac cctgtggcac
     8221 tgaacaaata cattactgaa tacctgacta caaagggcgt gtttgaagat gaaggaacaa
     8281 atcagagcgc aactgatact ctctcgtcgc cagtaccaga aactgatgca gtggaaacgg
     8341 caattccgga caacgaaaaa accgaatgca aagtggaagt cgaaccatct gtagagcgtg
     8401 aggggccgtt ctacttcctc ttcaccgaca aggatgacga aaaatacggt cgcgcaaaca
     8461 aactttctgg tctggataag gcgctggctg ccggggctac tgaaatcacg aaagaagaat
     8521 atttcgcccg caaaaacagt acatactcag gttcacaaca aaatactggt gcatctgaca
     8581 cgaccgcaca accagagccg gtaaaagtta ccgctgacga agtaaacaaa attatgcagg
     8641 cagccaatat cagccagcct gacgccgatg aactgcttgc agtatcacgt ggtgaatttg
     8701 ttgaagggat tagcgccccg aatgatccga aatgggttaa ggggatccag acccgcgatt
     8761 ctgtaaacca gaaccagcat gaatcggaac ggaactacca aaaagcggaa caaaacagcc
     8821 caaatgcgtt acaaaacgag ccagaaacga aacagcctga accagtggcg caacaggaag
     8881 tggaaaaagt ctgcaccgcc tgcggtcaga ccggcggcgg caactgccct gattgtggcg
     8941 cggtgatggg cgacgcaaca taccaggaaa cattcgatga agagtatcag gtgaagttca
     9001 ggaagatgat ccggagaaaa tggaaggcgc tgaacatcca cacaaggaga atgctggcgg
     9061 caatcagcat cacgatagcg ataatgaaac tggcgagacg gtagatcact caattaaggt
     9121 gaacggtcat cacgaaatca catccaccag caggacgtgt gaccatctaa tgataggcct
     9181 tgaaaccatg ggaaaaaatc ctgatgcccc gattatctca ataggtgcaa tatttttcga
     9241 tccgcaaacc ggaggtatgg gaccggaatt tagtaagact atcgatctgg aaactgctgg
     9301 cggagtcatt gatcgtgacg tcattaaaag gtggctgaag caatcacgtg aagcgcagtc
     9361 tgccattatg accgatgaaa tcccgttaga tgatgcactg ttacaattgc gggaatttat
     9421 cgacgaaaac tccggtgaat tttttgttca gatatgggga aatggagcca acttcgacaa
     9481 cgtaatttta cgccgttcat atgaacggca ggggatcccc tacccgtggc tttactacaa
     9541 cgatcgcgct ggacgatgcc cgtcaccagg caaaacaagt ttcagcaatc tggcaaaaac
     9601 tgatcccgag tcaggctgat ttttaatgtt caaccccggt cgtcgtccgc aagctataat
     9661 ggcggcggcc atgattagcg aacaacgctc atggcaagac ttattctgct cactgagtgg
     9721 gcaaaagagg aattcagtga accggtccct actccgggca cgttaagtaa atacgctaaa
     9781 gccggaatga tatttcctct ccccaaaaaa gttggaagac actggcgagt ggatcctcga
     9841 gctcgctttg tcggaatggt aaacaagccg gaagtgatcg ccacagatca ccctgctttg
     9901 aagaggatac tggaagatgg cgcgcccacg aaaatataaa actgaagttc cgggattatc
     9961 tccgtatttt gacaaaagaa ataacaaagt ttactggcgt tacaggcatc ccataacagg
    10021 aaaaaatcac gggctcggca gtattgacca gaaacaggca gaagctattg cagcagaagc
    10081 gaacagccgt cttgccaggc aacaaatgga acaaatgctc actctgcagg agaaaattat
    10141 tcgtgatacc ggtggttcat caaccgtttc tgtttttctg aatagttacc gaaaaattca
    10201 acaggaaaga tatgaaaacg gagagatcaa actcaacaca ctgaaacaga aagcatcccc
    10261 tctcagggta tttgatgaac gttttggtac cagaccatta gatgccataa ccgtaaaaga
    10321 tgtggtatcg gtgctggaag attacaaggc aaaaggacat aacagaatgg gacaaatttt
    10381 caggaaagta ctgattgatg ttttccggga ggcccagcaa acgggcgatg tctcgccagg
    10441 ctttaaccct gcagaatcgg caaaaaaacc acaggtacgg atatcacggc aacgactgac
    10501 ctttgatgag tggatgatga tttataatgc agcggaaaag gatggttact ttttacagcg
    10561 tggtatgctg ctagcgctga tgacaggcca gcgcctttca gatatttgca aaatgaaatt
    10621 ttcggatatc agggatggtt atcttcatgt cgaacagcaa aaaacaggaa cccggattgc
    10681 catccctctg gctctgcgtt gcgataaatt aaatctcacc ctggatgatg tagtgtcatc
    10741 ctgtcgcgat tgcgttctta gtccgtggtt attgcaccac catcatgcta aagggacagc
    10801 taagcgcggc gggatggtta agccagcaac gttgacagtt gcatttaaaa aagcccggga
    10861 ttctgtggat tacaactggc gcgctaatgg cactccaccc tctttccatg agcagagatc
    10921 tttatcagag cgattgttca gggagcaggg aattgatacc caaattttgc tgggtcattc
    10981 gaatcaaaaa atgaccgata tttacaacga tgtacgcggt aaagagtgga aaaaactggt
    11041 catttgatga ccagttttgc agaggagttt tgcagagatt ttgcagagaa aattaaaagc
    11101 gatatccggc agagaacata aacacccacg gatccagtcg taccgagtct ttcacggtag
    11161 taacaccaga tttatacttg gccgtggtat cgatatccat gtaccacact gacatgttaa
    11221 ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct ccccaggagt
    11281 ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat ccattatcaa
    11341 agaaggtggt gtagttaata cctgcaccaa cgtaaggacg gaatttgctg ctggcatcac
    11401 caaaatacca ctgcgccatc agtgttggcg gcagatgatg aacggttgca atatcgccgg
    11461 tcgcccgggt gccgatttta tggcggaacg gcgttgctgc cagtaattcc acaccaatgt
    11521 tgtcggtcgc catataagta aacgtcaggc ccagttgagt gttattggtc acgctgaatc
    11581 cacccaaact tcctaacgta ccaccagcac cttctgttgg acgtacggtt gcagaacctg
    11641 cacgcataaa aaattcgccg gcttcatgcg caaaagcact gccagagaga agagttgcta
    11701 ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg ttataaaaac
    11761 gtgacgaata tacccacatt ggggtaacaa atgatcctac acagatcaca ttacatatgg
    11821 taattcaccc tttattgatc tggattaatt tagaagttgc attgaaaatc caggttaatg
    11881 tgatttaaat caatttttat attttcgcta cgcaaatatt aactcgctcg ccacttgcag
    11941 cggtttaccc acgcctgaag gccagcaatt acctccgctt tacaaagata tgcttttcca
    12001 tacaagacct gatgctgcca cccggggctt aaccagggta caattgcccg ctaattaaca
    12061 cctgcataaa actcaaggag agtgcatgtc tatcacggcg cagtccgtat accgtgacac
    12121 cggaaatttc ttccgtaatc aatttatgac cattctgttg gtatcgttgc tatgtgcgtt
    12181 tatcacagtg gtgttagggc atgttttctc acccagtgat gcacagcttg cgcagctcaa
    12241 tgacggcgta cccgttagcg gcagtagtgg gttgttcgac ctggttcaga atatgtcacc
    12301 ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc actttttcag gattaatcgg
    12361 taacgccatt ctcgccgggg gcgtaatatt aattatccag ctggtgtctg cgggtcagag
    12421 agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata ttgccaaagt tatttattct
    12481 gatttttctg actacccttt tagtacagat tggcatcatg ctggtggtcg ttccggggat
    12541 tatcatggcc attttactgg ctctggctcc agtgatgctg gtacaggaca aaatgggcgt
    12601 ttttgcctcg atgcgtagca gtatgcggct gacatgggcg aatatgcgtc tggtggcacc
    12661 cgcagtactg agctggttgc tggcaaaaac actgttgctg ctttttgcct cttcttttgc
    12721 cgcattaacc ccggaaattg gtgccgtact ggcgaacacc ttgagcaacc tcatttcagc
    12781 cgtattgctc atctatctgt tccgcctgta tatgttgatt cgccaataac cttaagcatt
    12841 tgattacgga atcgtaaaat gaagcagttt cttgattttt taccgctggt tgtctttttc
    12901 gcgttttaca agatttatga catctatgcg gctactgcgg cgctcatcgt cgccacggcg
    12961 attgtgctta tatatagctg ggttcgcttt cgtaaggttg agaagatggc cctgatcact
    13021 tttgttctgg tggtcgtctt cggtggctta acgctgttct tccacaatga tgagtttatt
    13081 aaatggaagg ttactgtcat ttatgccctg tttgcgggtg ccctgttagt cagccaatgg
    13141 gtgatgaaaa agccgctaat tcagcggatg ctgggtaaag aactcaccct gccgcaatcg
    13201 gtatggtcga agctgaatct ggcctgggct gttttcttta tcctttgcgg tctggcaaac
    13261 atctacatcg cattctggct gccgcaaaat atttgggtca actttaaagt ctttggcctg
    13321 accgccctta ccttaatctt cacattgtta aacggtatct atatctaccg ccacatgccg
    13381 caggaagata aatcctaacc agactgccag tccttcaggg gctggcgctg tatctcctct
    13441 gttctcctca ggaaaatcat agtagcatcg cgcctgtgat tttcctttta agtcggtttt
    13501 accatgtcta caacacataa cgtccctcag ggcgatcttg ttttacgtac tttagccatg
    13561 cccgccgata ccaatgccaa tggtgacatc tttggtggtt ggttaatgtc acaaatggat
    13621 attggcggcg ctattctggc gaaagaaatt gcccatggtc gtgtagtgac cgtgcgggtt
    13681 gaaggaatga ctttcttacg gccggttgcg gtcggcgatg tggtgtgctg ctatgcacgc
    13741 tgtgtccaga aagggacgac atcagtcagc attaatattg aagtgtgggt gaaaaaagtg
    13801 gcgtctgaac caatcgggca acgctataaa gcaacagaag cattatttaa gtatgtcgcg
    13861 gttgatcccg aaggaaaacc tcgcgcctta cccgttgagt aataatcaaa agcctccggt
    13921 cggaggcttt tgactttatg cttactgaat ttcggtagtg ccgttaattt taaacaggat
    13981 attcaccaca atcccactgc ctggcttacc cggctcataa cgccatctgc gcatcgcatt
    14041 tttcacctca cgctcaaaca tgtttgcagg cttggctgag aggatttgta cgttatccac
    14101 gcgaccatct ggcgtgacat caaatttaac tttaacctgc ccttcaatgc gcaatgcctg
    14161 tgctcgtgcc ggatactgcg gctgattacg gcttaatgcg cgtggtcctg aagccacact
    14221 ggtaaccggc ttgctggttg cagccgttgc cgtacttgat gtcgggcgcg cgggtgccgt
    14281 attttcaaac ggtgatgccg gacgcgactc tacgggtttg acatcgcgtt ttggctgctc
    14341 cacctttttc accggcttag gttttggctt aggtttcggc ttcggctttt caatgaccac
    14401 cggagcttct tttggcggtt cagggatcgg ctcaggttcc ggttctggct ctaccaccgg
    14461 ctccggtggt ggctgaacgg cttgtggcgg ttcaagatca gcaggcgcaa ccatcgtgac
    14521 agaaatcggc tgcgcgggcg caggtagttc aataacctga tgtaccgagg tatagagcaa
    14581 acccgccaca acagcaccat gaatgcagac cgaaagtaac gtcggccagg ggaagcggcg
    14641 aggtaaatca agggtcattg aagtcataat catttcagta gaaaaaccag gccccgattt
    14701 taaatgcaaa tagcaatcat attcaataag gcaagacgat caggatcagt gaaaatgtca
    14761 tttttggcgt aattgacctt actttatgca tggtcttaac aaagacatca tctttccgtt
    14821 gcagatcttc gccctttcaa ataacgtact ttacaacttt cccgaacaag gagttgtgcc
    14881 cgtgttgtat gttatttacg ctcaagacaa agctgatagc ctcgaaaaac gcctttccgt
    14941 tcgtccggca catttagcac gtttacagtt actgcatgat gaaggtcgtt tgttgaccgc
    15001 cggtccaatg ccagcggtag acagtaacga tccaggggct gcgggattta ctggctcaac
    15061 cgttattgct gaatttgagt cgctggaagc agctcaggcc tgggccgatg ctgacccgta
    15121 cgtagcggca ggagtctatg aacacgtttc agtgaaacca tttaagaaag tgttctgaat
    15181 agaaattcaa tatttaaggg cacggttttt gcaaccgtgt tcttgtttgg cctttcctta
    15241 taggtcggtc agtgcattca ccaccacaag cagataataa ctttatctgt atttctttat
    15301 atgatgtgaa catattcagg aaaatgccct ttcctgacgc agagttgctc actcaaaacc
    15361 tttgattact gcggataaag tcatctacta ttgtttaaca cattcacgcg ctgcatgtat
    15421 gttcagaaaa tgtgcacgtt gagataatta aacaaaattg tggttacaga ggtatcagtg
    15481 agtcactgga ctacattcaa acaaacagca acaaagttat gggtgaccct acggcacgac
    15541 attctcgcgc tggccgtctt tttaaatgga ttgcttattt ttaaaacaat ctatggtatg
    15601 tcggtcaatt tgcttgatat tttccatatc aaagcatttt cagagctgga tctctccttg
    15661 ctggcaaacg ccccactatt tatgctcggc gtatttcttg tcctgaactc cattggctta
    15721 ctgttccggg caaagctcgc atgggcaatc agtatcattt tgttgttgat agcgctaatt
    15781 tacactctgc atttttatcc ctggctgaaa tttagtattg gattttgcat ttttacgctg
    15841 gtgtttttgc tgatactgcg caaagacttc tcccacagta gcgcagcagc cgggacaatt
    15901 ttcgcattta ttagtttcac gacgttactg ttttactcca cttacggtgc gctttattta
    15961 agcgaaggtt ttaatccgcg aatagaaagt ttgatgaccg cgttctattt ttcgatagaa
    16021 accatgtcaa ccgtcggcta tggcgatatt gtccctgttt ctgaatcagc acgattgttc
    16081 actatttcgg tcattatttc cggcattacc gtttttgcca catctatgac ctcaattttt
    16141 ggtccgctta tccgcggggg attcaacaaa cttgtaaaag gaaacaatca tacaatgcat
    16201 cgtaaagatc attttattgt ttgcggacat tcgattctcg ccatcaatac gattctgcaa
    16261 ctgaatcaac gcggacaaaa cgtaacggtt atcagcaact tgcctgaaga tgatatcaag
    16321 caacttgagc aacgcttagg cgataacgcc gatgttatcc ccggtgacag taatgacagt
    16381 tcagtgttaa agaaagcggg aatcgatcgt tgccgggcca ttctggcgct gagtgataac
    16441 gatgcagata acgcgtttgt tgtactctcg gcaaaagata tgagcagtga tgtcaaaaca
    16501 gttctcgccg tcagtgatag caaaaacctg aataagatta agatggtaca tccggatatc
    16561 attctctcac cacaactgtt tggcagcgaa attctggcgc gagttttaaa tggtgaagag
    16621 attaataatg atatgctcgt ttcaatgttg ttgaactccg gtcatggcat tttcagcgat
    16681 aacgatgaac aagaaacgaa agctgacagt aaagaatcgg cgcaaaaata gagtttaaag
    16741 acggaagagt aaacttattg ggcaccgcaa tggtgcccag tacatttaga tattgtagat
    16801 cgcaaacaat aacgagttac gctgtttgtt tagaatgcac ttcctgatgc tgtggatacg
    16861 catatttcgg cgcgactgcc cctcaagcca acgcgattta cgtcggctag cctgacgctg
    16921 catgcgccag cgccccactt ccgttctact ccgcttcatg ttaactactc tatgcaacaa
    16981 cagaatagtc attatacccc cgcccctgac gctgaccagt gcttttcccg cgtttttatt
    17041 tgccagatga atccatatgc gtaaactcat aacaatgcgc tttcaaaagg atttctaatc
    17101 tatgacaacc gtttatacgt tggtgagttg gttggccatt ctgggatact ggttgctcat
    17161 tgcaggcgta actttacgca ttctaatgaa acgacgcgca gttccctccg cgatggcctg
    17221 gctgttgatt atttacattc tgccgttagt cggaattatt gcctatcttg ccgttggcga
    17281 gctccattta ggcaaacgcc gcgctgagcg cgccagagcg atgtggcctt ccaccgcaaa
    17341 atggcttaac gaccttaaag cctgtaagca tatcttcgcc gaagaaaata gcagtgtcgc
    17401 tgcgccatta ttcaagcttt gcgagcgtcg tcaggggatc gctggggtca aagggaatca
    17461 gctacaactg atgaccgagt cagatgatgt gatgcaggcg ttaatccgcg acatccagct
    17521 cgcgcgccat aatattgaga tggtgtttta tatctggcag cccggcggca tggcggacca
    17581 ggtggctgaa tcattaatgg ccgctgcacg gcgtggtatt cattgccgat tgatgctcga
    17641 ctccgccggg agtgtggctt ttttccgcag cccgtggccc gagctaatgc gtaatgccgg
    17701 tattgaagtt gtcgaagcct taaaggtcaa tctgatgcgt gtgtttttac gccgtatgga
    17761 cctgcgccaa catcgcaaga tgatcatgat cgataattac atcgcgtaca ccggcagcat
    17821 gaatatggtc gaccctcgct acttcaaaca agatgcgggc gtagggcaat ggattgatct
    17881 gatggcgcgt atggaaggcc ccatcgccac cgcgatgggg attatttatt cctgcgactg
    17941 ggagattgaa accgggaaac gtattctgcc gccgccacca gatgtcaata ttatgccgtt
    18001 tgagcaggcc agcggtcaca ccattcacac aattgcttct ggccccggct ttccggaaga
    18061 tctcattcac caggcattat tgactgctgc ttattcggct cgtgaatatt tgatcatgac
    18121 aacgccctat tttgtgccaa gcgatgattt acttcatgcg atttgcacgg cggcgcagcg
    18181 cggggtggat gtcagtatta tccttccgcg aaaaaatgac tcgatgctgg tcggctgggc
    18241 cagtcgcgca ttctttacag aactgctggc tgctggggtt aaaatttatc agtttgaagg
    18301 cgggttactg cataccaaga gcgtgctggt cgatggagaa ctaagtctgg ttggcaccgt
    18361 taaccttgat atgcgtagtc tgtggctaaa tttcgagatt accctggcaa tcgacgataa
    18421 aggttttggt gcagacctcg ccgccgttca ggacgattat atttcgcgtt ctcgtctgct
    18481 cgatgcccgt ttatggctaa aacgtccatt atggcaacgt gtcgccgagc gactgtttta
    18541 cttcttcagc ccgttgctgt aaaacgtcgc caacagaggt taaacaggta gtgactatgg
    18601 atatggatct aaacaatcgc ctgactgaag atgaaacgct tgagcaagcc tacgatattt
    18661 ttctcgaact ggctgccgac aatctcgatc cagctgatgt tctgctgttc aatcttcagt
    18721 ttgaagagcg cggcggcgcg gagttattcg atccggcgga agactggcag gaacatgttg
    18781 atttcgattt gaacccggac ttttttgccg aagtggtgat tggtctggcg gacagtgaag
    18841 atggtgaaat caacgatgtt ttcgcgcgta ttctgttatg ccgcgaaaaa gatcacaaac
    18901 tttgccatat catctggcgg gaatgaataa tcgcttttgg cgtgaaaaag ggctgacaac
    18961 tgtcagccct tattgtgttt aaagcggatc gactttcagg caagaaacgg cgtgtctgaa
    19021 actcccctcc agcacaggac gtgttttggc acactccgga ccggcaatcg gacaacgggt
    19081 acggaaaaca caaccggaag gcgggttgat cggcgacggt aattcccctt ccagtaactg
    19141 gatggttttg ttcttctcca gatccggatc aggtatgggg actgccgaca tcaatgccct
    19201 ggtgtaagga tgtagtggat tgtggtagac ctcatcatag gtccccagtt ctaccgcatg
    19261 gccgagatac atcactaaca cacgatcgga aatgtgtttt accacggcca ggtcatgagc
    19321 gataaaaatt aatgacaatc ccatctcacg ttgcagctgc tggagcaggt tgaccacctg
    19381 cgcctgaatt gacacgtcca gcgccgaaac cggctcatcg cagataatca gcttcggttc
    19441 aagaataaga gcacgagcaa tcccgatacg ctggcactgc ccaccggaga actcatgcgg
    19501 atagcggtta atcaggttag gcaataaccc gactttcagc atcatcgcct tcacgcgctc
    19561 gcgaacttcc tggcgtgaca ttttcggatg ataagtacgc agtggttcag cgatgatctc
    19621 gccgatggtc atacgcgggt tcagcgatgc caacggatcc tggaaaatca tctgaatatc
    19681 actgcgaacg gcacgccatt catcgggctt catgcccagc aactctttac ctaaccaggc
    19741 aacatgaccg tcggtcgcct tgaccaaacc gatgatggcg cgagcaaagg tggacttacc
    19801 gcatcccgat tcccctacca cacctaatgt ttccccttca tacaggcgaa gagtgacacc
    19861 atcgacggct ttgagcgttt tcggcggttg ccagaaccac tgtttgccat ctttgatttc
    19921 aaagtgcact ttcagagcag cgatttcaag gaggactttt cttccttcag ttacagcatt
    19981 cataacagtt cctccaccgg tttaaagcaa gcacgcagac ggccaggcgt aaactcttcc
    20041 agcggcggtg cgctactaca aatttccatc gcatgtggac aacgtggctg gaacgggcaa
    20101 cctttcggta atcgcagcaa gtttggcgga ttacccggga tggtcaacat tgtttcacct
    20161 tccgcatcga gacgcggcac cgcgttgagc aaaccgatag aataaggatg aacgggttga
    20221 taaaagacat cgcgcgcatt gccatattcc atcgtgcgcc cggcgtacat taccagcact
    20281 ttgtcgcaga tccccgccac cacgccaaga tcgtgggtaa tcataatgat ggcggtatta
    20341 aattcccgct tcagttcatt caatagcgtc atgatctgcg cctgtacggt gacgtccagc
    20401 gcagtcgtgg gttcatccgc aatcagcagc ttaggtcgac atagcaatgc catcgcaatc
    20461 atgactcgct gacgcatgcc gccagaaaat tcgtgcgggt acattttcat gcgtttacgc
    20521 gcttccggca tttttaccgc atcgagcatc cgcaccgatt cttcaaacgc ctcagctttg
    20581 ctcatgttct tatgcagcat cagcacttcc atcaactgct caccgacgcg catataggga
    20641 ttcaacgaag tcattgggtc ctgaaaaatc attgagattt gttcagctcg cagtttattg
    20701 agttcacgct ctggcaaatt gaggatttca cgcccattga aggttgccga tccgccaata
    20761 cgtccgttgg cagccaacag gcccatcaac gcaaatgcgg tttgcgattt gcccgaacca
    20821 gactcaccta cgatgcccag cgtttctccg gcacgtagcg aaaaattcaa atcattgacc
    20881 gccgtgacgt cgccatccgg ggtactaaag gtgacacgca aatctttcac gttcagcagt
    20941 gcgtcagcct gttgttgtgc gagcggcaca gttgcagttt caattacgct catggctgca
    21001 ctccttaacg atctttcggg tcgagggcat cacgcaagcc atcgccgata aagttgaaac
    21061 aaaacagcgt taccacgagg aatcccgcag ggaacaacag taaccatgga gagacttcca
    21121 tcgagttcgc gccatcactc agcaatgcac cccagctgct taacggctct tgcgtaccta
    21181 atcccaagaa gctaaggaaa gattcaaaga ggatcatgct gggcaccaga agcgacgcat
    21241 agaccaccac cacaccgagt acgttcggca caatgtggcg aataacaatg cccggcgtcg
    21301 atacaccgcc aacttgtgcc gcctcaataa actctttgcg cttcagactc agggtttgcc
    21361 cacgcacaat acgagccata tccagccagg aaaccatgcc aatcgccacg aaaatcagca
    21421 ggatgttttg accgaaaaag gtcaccagca aaatgacgaa gaacatgaat gggaaggagt
    21481 tgaggatttc cagcagacgc atcattaccg aatccacttt accgcccaga taaccggaaa
    21541 gcgaaccata aagtgtcccc acgaccactg ccaccagtgc cgcagcaaca ccgaccatga
    21601 gtgagatacg cccgccaatc gcaacgcgca caagcaggtc gcgaccggat gagtcagtac
    21661 caaagtagtg accggactcc atatccgggg cgctggacat catcgcccag tcagtatcgt
    21721 cataggcaaa ctgcgaaagc atcggtgcca ggattacaaa taacgcgatc agcaccagca
    21781 ctatcagact ggcaaccgcc gcacggttat gcataaaacg tcgacgtgcg tcctgccaca
    21841 agctgcgccc ttcgacctcc agcttttcac tgaaattttc cagcgtctcg ctgtttttct
    21901 tacttaacat catcgcgagc tccagtatca gtaacggatt ttcgggtcga taaccgcata
    21961 tagcacatcg acaatggcat taaacaaaat ggttaacgcg ccaaccagaa tggtcagact
    22021 taacactaag gaatagtcac ggttcaatgc gccattaacg aacaattgcc caatccccgg
    22081 taaaccataa atggtttcga tgaccatcga accggtaata atgccgacaa atgcgggccc
    22141 catataggag agcacgggta acagagcagg ttttaatgcg tggcgtaaaa tgatccgccg
    22201 cataggtaac cctttcgccc gggcagtacg aataaagttg gagtgtaaaa cctcaatcat
    22261 agaaccacgg gtaatacgcg caatactggc gatataggcg agtgataacg ccaccattgg
    22321 caatatcatg aatttaagcg ctccaccatt ccagccaccg cccggcagcc aatgcaaaat
    22381 gatcgcaaat atcatgacta ataatggcgc aaccacaaaa ctggggataa caaccccggt
    22441 cattgccagc cccatcacgg tatagtccca tttggtgttt tgctttaatg cggcaataac
    22501 gccagcacta acacccagta ttaccgcaag aaaaaatgct gcggctccca gtttggcaga
    22561 aacgggaaaa ctggatgcaa ccaggtcatt gaccgaataa tctttatatt taaacgatgg
    22621 accgaaatcg ccatgcgcca gttgtttcag gtagctgaaa tactgtgtca tgatcggatc
    22681 attaagatga tatttcgctt cgatattggc catcacttcc ggtggtaaag tacgttcgcc
    22741 ggtaaaagga ctgcccggag cgaggcgcat cataaagaac gaaatagtaa taagaataaa
    22801 tagcgtcgga atcgcttcca gacagcgacg tagaataaat tttaacattg ccctaccttc
    22861 tggcctgtgc cttctgtaat gcgataaaaa tcagacaccg tggagcagga cactcctgcc
    22921 ccacgtattg ccattagtgc ttcacaatgt acatattccg ggtgtaggta ttatccagcg
    22981 gatctttgcc ggtatagcca ccaacccacg gtttcaccaa acgcgcattc acgtagtaat
    23041 aaacaggaac aatggccgaa tccttatcca gctgttgttc tgctttagtg tacagagctg
    23101 tacgctgcgc ctcgtcagtc actttcagcg tttccgccat aatgctgtca aaggccgggc
    23161 tcttataatg cgcggtattc attgagctat tcgacagcat ggtgttcagg aaggaagttg
    23221 gttcgttgta gtctgcacac cagcctgcac gggccacatc aaaagtgccc tggtgacggg
    23281 tgtcgaggaa cgttttccac tcctggttaa ccagtttgac gtttacacca atgtttttct
    23341 tccacaatga agaggcagca atcgccagct ttttatgcag atcggaggtg ttatacaaca
    23401 ggttgatggt caacggtttg tctgcggtat aacccgcttc agccagcagt tttttcgctt
    23461 cttcgttacg tttttcctgg ctccagccaa accattccgg ctgagtcaat tttgcgccat
    23521 cagtatatgg tggagtgtaa ccataggcgg gcatgtcgcc ctgcgctttc actttattaa
    23581 caatgatatc gcggtccata cccaatttca gtgcggtacg cacacgcacg tcattaaatg
    23641 gcggtttctg gttgttaatt tcgtaatagt aagtgcacag gtatggatca acgtgaactt
    23701 cgtccgggat ctcttttttc agcttctgga acaattcgat cggcatgttg ttataagtca
    23761 tgtcgatttc accactacgg tagcggttga catcggtaac ttcagaagca ataggcaaat
    23821 aggttacctg gttaataacg gttttcgcgt tgttccagta ggtcgggctg cgttcaagaa
    23881 cgattcgttc gtttacgacc caatctttta aggtataggc accgttggtg acgatattac
    23941 caggctgggt ccatttttcg ccgaatttct cgatagcggc ttttggcacc ggtgaagttg
    24001 atgggtgaac aagtaattta tagaagtacg gaacaggttc acttaaggtg acttctaatg
    24061 tgtgatcatc aatagctttc acgccgagat cggtaatcgg ttttttccct tcaagaattt
    24121 catcaatacc ggcgatatgc ccatattgca gataactggc atacggagaa gcagtgtttg
    24181 gatcaacaga acgttgccag ctatacacaa agtcttgtgc tgtaactggc gtgccatcag
    24241 accatttcgc atctttacgc aaatggaagg tccagacttt cgcgtcttta ttatcccatg
    24301 attcagcgac gccaggtgcc ggatgaccgt caagatcgct gaccagtaag ccttcaaaca
    24361 ggtctcggct gatattagac tccggaacac cttcaatttt gtgcggatct aatgactgaa
    24421 cttctgaacc attgttacgt accagcgttt gtttttccgc cagtgtgaca cctgcgggta
    24481 catcagctgc cagcgcgaca ttccctgcca taagcgcagc caaaacgcca gctgctacta
    24541 aacttctctt ggtgatgttg gtcattgttt tttggactcc ctcattataa ttactggtat
    24601 taaccagcat gtgtaatccc ggattgggac cctgcacagg ctcggagact ttctgctgtc
    24661 aggttacttt atcgttacgt attactatca ccgactttat ttattctggt gattcagacg
    24721 atcaccttat gtcgattcgt actgtgtctc ccctgtcgag acacattatt ctttcgcgac
    24781 gatctttaat tgttaattat tctcattacc aataaagaat cgcctggcgg ttgcccggaa
    24841 agtaccaaat gcggttctta ctcgccaata cattttgcaa aattgttaag caattctctt
    24901 ttattgaccg tatcagaatc tatagcttgc atccatgcct taacaaacat ctttcaattc
    24961 aatcaaatag aggttggtta agcatgtctc tcttttctga ttacagttcc agtagtgaaa
    25021 tgcacaataa tttaacaatt gattattatt tagcactttc ctctaccaaa ggtagtggaa
    25081 ttactaatat catttcaatt attcttcagc aagcccagga ttatgatgtg gcgaaaataa
    25141 catgatggtg aggagaagcc gttaaggaaa tccctataac taactgtaat ttgagaagaa
    25201 tatttttacc tattcctttt gcttttgata actgcgcagg aacaaagtaa aaataatgac
    25261 gaagtaaacg gagcatggca gctccgtttc atttgaaagg aattaattaa gcaggccggg
    25321 gaaaatcccc ttaataccag taacgataaa ttcaatcccc aacgccatca gcaatagccc
    25381 cataatacgc gtaatcacgt tgatgccggt ctggcgtaaa actcgtacca gccacggtgc
    25441 catgcggaac aatccccagc aacataaagc gaacaatgca atagccacaa agaaaccaaa
    25501 cagatagcta atgctgtgat aacgcgtacc ccagacgatg gtagaactga tcgcccctgg
    25561 ccccgccatc aacggcaacg ccaatggcac cacaccaatg ctttcacgta ccgcggtttc
    25621 tgatttttct tgcttgttct gtttatcctc accaagcttg ccgctgatca tcgacatcgc
    25681 tattgtcacc accaggatgc caccggcgat acggaatgaa tcaattgata taccaaaaag
    25741 ttgtagaatc gtgtcgccga gaaaaagcga gatccacaag ataatggcca cagacaggtt
    25801 ggctgtaagg ttagttttgt ttcgcgctgc cgctgtctga taactggtca tgctgataaa
    25861 gacgggaata atccctaccg ggttgaccag cgcaaataac ccgatgaaaa atttgaagta
    25921 aacgggaaaa tcaaaaaggg tctgaatcac ggttagctcc gaagcaaaag ccggataatg
    25981 ttagccataa ataaggttga aaagacgcgc tgacaatacg ccttttgaca gcatttttca
    26041 cctcctaact acttaaaatt gctatcattc gttattgtta tctagttgtg caaaacatgc
    26101 taatgtagcc aacaaatcat actacaattt attaactgtt agctataatg gcgaaaagcc
    26161 atgctgaaag gtgtcagctt tgcaaaattt tgatttggat cacgtaatca gtacccagaa
    26221 gtgagtaatc ttgcttacgc cacctggaag tgacgcatta gagataataa ctctaatgtt
    26281 taaactcttt tagtaaatca cagtgagtgt gagcgcgagt aagcttttga tttccatcgg
    26341 ttaagcaaat catcaccgca ctgactatac tctcgtattc gagcagatga tttactaaaa
    26401 aagtttaaca ttatcaggag agcattatgg ctgttactaa tgtcgctgaa cttaacgcac
    26461 tcgtagagcg tgtaaaaaaa gcccagcgtg aatatgccag tttcactcaa gagcaagtag
    26521 acaaaatctt ccgcgccgcc gctctggctg ctgcagatgc tcgaatccca ctcgcgaaaa
    26581 tggccgttgc cgaatccggc atgggtatcg tcgaagataa agtgatcaaa aaccactttg
    26641 cttctgaata tatctacaac gcttataaag atgaaaaaac ctgtggtgtt ctgtctgaag
    26701 acgacacttt tggtaccatc actatcgctg aaccaatcgg tattatttgc ggtatcgttc
    26761 cgaccactaa cccgacttca actgctatct tcaaatcgct gatcagcctg aagacccgta
    26821 acgccattat cttctccccg cacccgcgtg caaaagatgc aaccaacaaa gcggctgata
    26881 tcgttctaca ggctgctatc gctgccggtg ctccgaaaga tctgatcggc tggatcgatc
    26941 aaccttctgt tgagctgtct aacgcactga tgcaccaccc agacatcaac ctgatcctcg
    27001 cgactggtgg tccgggcatg gttaaagccg catacagctc cggtaaacca gctatcggcg
    27061 taggcgcggg caacactccg gttgttatcg atgaaactgc tgatatcaaa cgtgcagttg
    27121 catctgtact gatgtccaaa accttcgaca acggtgtaat ctgtgcttct gaacagtctg
    27181 ttgttgttgt tgactctgtt tatgacgcag tacgtgaacg tttcgcaacc cacggcggct
    27241 atctgttgca gggtaaagag ctgaaagctg ttcaggacgt tatcctgaaa aacggtgcgc
    27301 tgaacgcggc tatcgttggt cagccagcct ataaaattgc tgaactggca ggcttctctg
    27361 taccagaaaa caccaagatt ctgatcggtg aagtgaccgt tgttgatgaa agcgaaccgt
    27421 tcgcacatga aaaactgtcc ccgactctgg caatgtaccg tgctaaagat ttcgaagacg
    27481 cggtagaaaa agcagagaaa ctggttgcta tgggcggtat cggtcatacc tcttgcctgt
    27541 acactgacca ggataaccaa ccggctcgcg tttcttactt cggtcagaaa atgaaaacgg
    27601 ctcgtatcct gattaacacc ccggcttctc agggtggtat cggtgacctg tataacttca
    27661 aactcgcacc ttccctgact ctgggttgtg gttcctgggg tggtaactcc atctctgaaa
    27721 acgttggtcc gaaacacctg atcaacaaga aaaccgttgc taagcgagct gaaaacatgt
    27781 tgtggcacaa acttccgaaa tctatctact tccgccgtgg ctccctgcca atcgcgctgg
    27841 atgaagtgat tactgatggc cacaaacgtg cgctcatcgt gactgaccgc ttcctgttca
    27901 acaatggcta tgctgatcag atcacttccg tattgaaagc agcaggcgtt gaaactgaag
    27961 tcttcttcga agtagaagct gacccgaccc tgagcatcgt tcgtaaaggt gcagaactgg
    28021 caaactcctt caaaccagac gtgattatcg cgctgggtgg tggttccccg atggacgctg
    28081 cgaagatcat gtgggttatg tacgaacatc cggaaactca cttcgaagaa ctggcgctgc
    28141 gctttatgga tatccgtaaa cgtatctaca agttcccgaa aatgggtgtg aaagcgaaaa
    28201 tgatcgctgt caccaccact tctggtacag gttctgaagt cactccgttt gcggttgtaa
    28261 ctgacgacac tactggtcag aaatatccgc tggcagacta tgcactgacc ccggatatgg
    28321 cgattgtcga cgccaacctg gttatggaca tgccgaagtc cctgtgtgct ttcggtggtc
    28381 tggacgcagt aactcacgcc atggaagctt atgtttctgt actggcatct gagttctctg
    28441 acggtcaggc tctgcaggca ctgaaactgc tgaaagaata tctgccagcg tcctaccacg
    28501 aagggtctaa aaatccggta gcgcgtgaac gtgttcacag tgcagcgact atcgcgggta
    28561 tcgcgtttgc gaacgccttc ctgggtgtat gtcactcaat ggcgcacaaa ctgggttccc
    28621 agttccatat tccgcacggt ctggcaaacg ccctgctgat ttgtaacgtt attcgctaca
    28681 acgcgaatga caacccgacc aagcagactg cattcagcca gtatgatcgt ccgcaggctc
    28741 gccgtcgtta tgctgaaatt gctgaccacc tgggtctgag cgcaccgggc gaccgtactg
    28801 cagctaagat cgagaaactg ctggcatggc tggaaacgct gaaagctgaa ctgggtattc
    28861 cgaaatctat ccgtgaagct ggcgttcagg aagcagactt cctggcgaac gtggataaac
    28921 tgtctgaaga tgcattcgat gaccagtgca ccggcgctaa cccgcgttac ccgctgatct
    28981 ccgagctgaa acagattctg ctggatacct actacggtcg tgattatgta gaaggcgaaa
    29041 ctgcagcgaa gaaagaagct gctccggcta aagctgagaa aaaagcgaaa aaatccgctt
    29101 aatcagtagc gctgtctggc aacataaacg gccccagaag gggtaatgcc agtcagttaa
    29161 ggatcagttg accgatccag tggctgtgta agaatccgga aacgctcact cgtttccgga
    29221 tttttttatg ctcattggac aggcccttga tttggtatcc cgttacgatt ctctgcgtaa
    29281 cccactgact tctctggggg attacctcgc ccccgaactc atctctcgtt gccttgccga
    29341 atcaggtact gtaacgctac acaagcgccg tcttcccctc gaaatgatgg tctggtgtat
    29401 tgttggcatg gcgcttgagc gtaaagaacc tcttcaccag attgcgaatc gcctggacat
    29461 catgctgccg ggcaatcacc ccttcgttgc ccccagtgcc gttattcaga cccgccagcg
    29521 tctgggaatt gaggctgtcc gccgcgtgtt cacgaaaaca gcgcagctct ggcataacgc
    29581 cacgccgcat ctgcactggt gcggcctgac cctgctggcc atcgatggtg tgttctggcg
    29641 cacaccggat acaccagaga acgatgcagc cttcccccgc cagacacatg ccgggaactc
    29701 ggcgctctac ccgcaggtca aaatggtctg ccagatggaa ctgaccagcc atctgctgac
    29761 ggctgcaaga acagcgaaaa tgagcttgct gagcaactta tagaacaaac cggcgataac
    29821 actctgacgt taatggataa aggttattac tcaccaggac tgttaaatgc ctggagcctg
    29881 gcgggagaac accgccactg gatgatccct ctcagaaagg gagcgcaata tgaaaagatt
    29941 agaaaactgg gtataggcga tcatctggtg aagctgaaaa ccagcccgca ggcacgaaaa
    30001 aagtggccgg gactgggaaa tgaggtgaca gcccgcctgc tgaccgtgac gcgcaaagga
    30061 aaagtctgcc atctgctgac gtcgatgacg gacgccatac gcttccccgg gacgtatacc
    30121 ggagctgatg cgcgatcttg caagtatggg acaaccagtg aaattaccga caagaaggga
    30181 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa
    30241 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt tttgttttcc
    30301 caaaacaacc tttccataaa ccggcagcat tgtccttcat catatttgcg acgatgtata
    30361 acgcctacac acagcgatat tgtactttac aggttacaag tcaatgtcgg tgtataagcg
    30421 ctctgtgagg cgtacagcgg cgttaaaaaa ctgccgagaa gggtgtatag cccggaagaa
    30481 gtgcgtaaaa cgaactgaca ggataaaagc gccctgctca ccctgtcagt aaagaaattc
    30541 ttattaatcg tggcgatgcc tttcctgaat agccgttaat gagccgactt ctaacgcttc
    30601 tttatagtgt ttacggcata cagaaacgta gcgttcatta ccaccaataa ccacctgctc
    30661 accttcgtta taaggtctgc ctgcttgatc aagacgcagc accatgcttg ctttacggcc
    30721 acaaaaacag atggttttta attcaaccag tttgtcggac catgccagta agtattgact
    30781 gccaataaat aattcacctc gaaaatcggt acgtagacca taacaaagta cgggtatatc
    30841 gagttgatcg acaacctccg ataattcata tacttgttgt ctggttaaaa actggcattc
    30901 atcaaccagt acgcaatgaa ttgcctgctg ttcatgttcc gcacgaatct catcaaataa
    30961 tgatgaattt tggttaaata attttgcagg tgatgacaaa cctatacgcg aactgacttt
    31021 cccggcacca aagcgatcat caatttctgc cgtatatacg acagtgcgca tgccgcgttc
    31081 ctggtaattg tatgaagatt gcaacaatgc tgtagactta cccgcattca ttgcggaata
    31141 gtagaaatat agctgtgcca tcagccacag gccctcaatg atatgaaata aagttggctg
    31201 gagtttatca taattcgtca gtttttcagc tcaacagtat gcacagaagt ggtatatgcg
    31261 ttctccctta cgaagccttg cataatcctt ctgagctatc attacaactg cctcgcttgt
    31321 tataagcggg taaatgactg ccggtaacta ttcacaatct ttaacctgtt gcgcatgtaa
    31381 tagccctctg ttgacctcca ggagatagtg caatactaag tccctgctct tattgcgact
    31441 gttctacttt tcatcattcg cttaataggg aattctcgta aacacgacta atacagaaga
    31501 ctgaaaggtc gtcagcctac gattatctcc ccataaaatg tgacatgaat caggaagttt
    31561 taacctcacg tgctgcgaaa tcatcggtgc aaatagggct atatgccgcg tcttttctgg
    31621 ctaattttat gaaaagatat ttattggcgg cacaaaataa agaacaattt tgaattcctt
    31681 acattcctgg ctattgcaca actgaattta aggctctatt attacctcaa caaaccaccc
    31741 caatataagt ttgagattac tacaatgagc gaagcactta aaattctgaa caacatccgt
    31801 actcttcgtg cgcaggcaag agaatgtaca cttgaaacgc tggaagaaat gctggaaaaa
    31861 ttagaagttg ttgttaacga acgtcgcgaa gaagaaagcg cggctgctgc tgaagttgaa
    31921 gagcgcactc gtaaactgca gcaatatcgc gaaatgctga tcgctgacgg tattgacccg
    31981 aacgaactgc tgaatagcct tgctgccgtt aaatctggca ccaaagctaa gcgtgctcag
    32041 cgtccggcaa aatatagcta cgttgacgaa aacggcgaaa ctaaaacctg gactggccag
    32101 ggccgtactc cagctgtaat caaaaaagca atggatgagc aaggtaaatc cctcgacgat
    32161 ttcctgatca agcaataatc ttttgtagat tgcacatgct aaaaatcccg ccatcggcgg
    32221 gattttttat tgtccggttt aagacaatat aataagaata agctataaaa aaacggcgtc
    32281 gattgctcaa caccgtttcg tggaaaacac cgatacggat gttacttctt aatgcccatc
    32341 tcttcttcaa gccaggcttt aaattccgtg ccaagggtgt tatgacgaat accgtattca
    32401 acgaaggcct gcatgtaacc taatttatta ccgcagtcat ggctcttccc tttcatatga
    32461 taggcttcaa ccgtttcttt ttcgatcagc atatcaattg cgtcggtgag ctgaatttca
    32521 tcaccagctc caggaggggt ttttgccagc aacggccaaa tatccgcgct aagtacgtaa
    32581 cgacccacaa tagcgagatt agacggcgca acatccgctt ttggtttttc aaccacgcca
    32641 accatcggta cgctttcacc cggcgctaat tcaacgcctt tgcaatccac aacgccatat
    32701 gcggtcacat cagcaaccgg ttcaaccatg atctggctat gacccgtttc atcaaagcgg
    32761 cggatcatct ctgccaggtt atcctgtgat aaatcggatt catattcatc cagaataacg
    32821 tcaggcaaaa taacagctac cggttcatca cccactaccg ggtgagcaca caataccgcg
    32881 tgtcccaggc ctttcgccag cccctgacga acttgcataa tagtcacgtg cggcggacaa
    32941 atagactgca cttcatcaag cagttgacgt tttacacgtt tttccagcat tgcttccagt
    33001 tcaaaactgg tatcaaagtg gttttcaata gagtttttag atgagtgagt aaccagcaca
    33061 atttcagtaa tgccagccgc aatacattca ttcacgacgt attgaattaa tggcttatcg
    33121 acaagtggca gcatctcttt cgggatggct ttcgtcgccg gcaacatcct ggttcctaat
    33181 cccgctacgg ggataacggc ttttttgact ttcgtattaa tggcagccat ttaaattctc
    33241 ctggactgtt cgtgttttga acgtgttcat atttctgtat cgcatcccag tatatcagca
    33301 ccccgaaggg gctaagttct gaaaaggagg cgagttagca taattaagac aattacttat
    33361 acgaatagcg acgatagtac caccagccga cattagcagg taatgcaaat ttagcccgtg
    33421 ttatcgtttg ctcattctgc agacaacatc aagcgcagcc gaccaccggt tccccatatt
    33481 tggcattgcc aggcatcgca tcgctggctc aattgattca aataagcgtt acctaaagtg
    33541 cctaacggaa caccattact gatttgcacc tgatgttcgc cggtatttaa cgtcgcattc
    33601 agacccgcag aaaccagaat gagatttttc agttcgcgat gataatagcc aactaatagc
    33661 ggaaactgcc ccggcagatt ggcctgacgg agtaaatggt ttacctgctt caataacgcg
    33721 cccaactctg gcaaccgttg attttgatgt gcaagctgtt cctgtaataa tccgttaaac
    33781 aatgcgcgta ataacaaggc agcaagtacg ccattatgcc cagctctggt gacatcaagg
    33841 caataaaatg ccagatcgtt ttccgaaagt gcggcaatat caagcaccag gccgggtttg
    33901 tccgcggcaa ctaattgacg ataattaacc cggcaatggg aaatcacctg ctgaactggc
    33961 ggttgtagtt cctgtaataa tttcgccgct gcagcagggt tatcaaccat tgcatcccag
    34021 tcgcgaaaaa gcctttcctc ttcctcaacg cgggaattaa acatgctggg atagagacag
    34081 gcaaaaacca tctcgcgcaa gcgattcaga tctttaaccg gtttcagcaa aacatcttca
    34141 acgcccagac gtaacgcttt ggcaatatct gccatatttt cagtggcaga tatcaccaga
    34201 actggtgtct ggtcacctct attgcgtata tgttccagca gtttaagccc gttcattcgt
    34261 ggcatcgcga tatcacatat catcaggtct ggagtgaaac ctcccagcaa ctcaagggca
    34321 tccaccccat cagccgccag taccgttgtc gctcccaatg aggaaaacca cgaatccaga
    34381 agcgagcgaa atacctgctc atcttcaaca atgagaatct gttttccgac caatggctgc
    34441 gtcatgttct ctcccctgac tggctttact caatagtggc acgctattgc cacctacgcc
    34501 tgtcagaatt aacttaagtg tagataaaaa ttctggtcaa atgttggtgc gtacaaacgg
    34561 caaaagttcg tccattttct tttccactgc cagctgcccg gccgcaatgg cagcgtgcgc
    34621 gcgatggaaa tcaagcgtag atatttgcgg gcaaacaggt tgaatcagaa tatcgggcgg
    34681 atcacctgcc atgcggttcc ttttaaggcg gttctccagc acctggatag aagtggtcat
    34741 aatctctgtt gccgttggcg ctgtcaccgc ccgacgtgtc gttatgctgc ctaacctttc
    34801 tttcagacgc gcatgccacg gcagagaatc accattctcg ctattttctt cactgacatt
    34861 aaaggagagc aaatcttgtt gcatcaaatg agcatcgtgc tgcaggtcga ccgctatcac
    34921 aatatcagcc cccaatgcac gcgtgaggga aataggaatt gggttaacta cagcgccatc
    34981 aaccagccag tagccgttat gcgcaacggg tgccattagt cctggaatac tgcatgatgc
    35041 acgaatagca agatggagat cgccttcagt aaaccataat tcacgcccag tacttaaatt
    35101 ggtggcaaca gccgcgaagc gacgggaaca attttcgatc tctgtttccg gcattatttc
    35161 gcgatattga ttgaagacac gctcgccgcg cagtaaccca ccgcgctgcc aggagagatc
    35221 catcaggcgt aaaacatccc aataactgaa agaggtcacc caatcttcca gcgcagataa
    35281 tcgatcgcat gcataggcag cgcccaccag cgaaccaatt gaacatcctg caacgatatc
    35341 aatttcaata cccacttttt ttagcgcatt aattacgcca atatgcgacc aacctcttgc
    35401 cgcgccagat cccagcgcca gccctatttt tatctttctc attattcccg caagattccc
    35461 ctggaatgca atcgtagcca catcgctact gctcggttaa catagtgcta ctcaggcggt
    35521 taatacgccg ttattgtttc cagggagatt atttgtgtct cagctttgtc cctgtggtag
    35581 tgctgtcgag tatagcctat gttgccaccc ttatgtgtct ggtgaaaagg ttgcacctga
    35641 tccagaacat ctcatgcgtt cgcgttactg cgcttttgtg atgcaagacg cagattattt
    35701 aataaagacc tggcatccat cttgtggagc aacggcgtta cgtgccgaat tgattgccgg
    35761 atttgcgcat actgaatggt tgggattgac cgtatttgaa cattgctggc aggacggcgg
    35821 caatatcgga tttgtcagtt ttgttgctcg atttaccgag ggtggcaaaa caggtgcaat
    35881 aattgaacgt tcgcgatttt tgaaagaaaa cggtcaatgg tactatattg acggtacacg
    35941 tccgcagttt ggccgcaacg atccctgccc ttgtggttca ggtaaaaaat ttaaaaagtg
    36001 ctgcggccaa taatggttga cggtacggtt tagcaaacac tctcaacaag gtttttccag
    36061 caatgcattc actccaacgt aaagttctgc gtactatttg tccggaccaa aaaggtctga
    36121 tcgcacgtat taccaatatt tgctacaagc acgagttaaa tatcgtgcag aacaatgaat
    36181 ttgtagatca tcgtaccggg cgctttttta tgcgcacgga actggaaggg atttttaatg
    36241 attccaccct gctggcggat ctcgatagcg cattgccaga aggctccgtg cgtgagctga
    36301 atcctgccgg tcgtcgccgg atagtaattc tggtcactaa agaagcgcat tgccttggcg
    36361 atttgttgat gaaagccaat tacggtggcc tggatgtcga aatcgcggca gttattggta
    36421 accacgatac tttacgttct ctggttgagc ggtttgatat tccgtttgag ctggtaagcc
    36481 atgaagggtt aagccgcaac gagcacgatc aaaagatggc ggatgccatt gatgcttatc
    36541 aacctgacta tgtggtgctg gcgaagtata tgcgggtatt aacgccggaa tttgtggcac
    36601 gcttcccgaa taagatcatc aatattcacc attcattcct gccagcgttt atcggcgcac
    36661 gtccttatca ccaggcctat gagcgtggcg tgaagattat tggcgcaacc gctcactatg
    36721 tgaatgacaa tctggacgaa ggcccaatca tcatgcagga cgttattcat gtcgatcata
    36781 cctacacagc tgaagatatg atgcgcgcag gtcgtgacgt cgagaaaaac gtcttaagtc
    36841 gtgcgctata caaagtactg gcgcagcgcg tctttgttta cggtaatcgg acgattattc
    36901 tttaatcgcc agcaaaaata actggttagc tttaatccgt tacggatgaa aattacgcaa
    36961 ccagttcatt tttctcaacg taacacttta cagcggcgcg tcatttgata tgatgcgccc
    37021 cgcttcccga taagggagca ggccagtaaa aagcattacc ccgtggtggg gttcccgagc
    37081 ggccaaaggg agcagactgt aaatctgccg tcatcgactt cgaaggttcg aatccttccc
    37141 ccaccaccat cactttcaaa agcccctgaa ttctcaaacg aatccgcaat taaatattct
    37201 gcccatgcgg ggaaggatga gaggcttcga ccaaggttcg actcgagtgc cagcgagaga
    37261 gcgttgccgc aggcaacgac ccgtagggcg aagcgcgcag cgctgagtaa tccttccccc
    37321 accaccattt cttcctgaaa tactcaaaac ctgttactcc gagctaattc tgcccaatgg
    37381 caccgcgttt accggaccta aaatatcctc ccctgtaggt cggataagcg aagcgcatcc
    37441 gacaactccc cccgcaccgt gcccataaaa aaaacccgcc ggagcgctga ggaatcccca
    37501 gtaatgggcg ggtaaaaaaa gacaggcata gagttttgtg atctactgac ttgtgcaacc
    37561 aattcaatga gatctcctct atgcctgctc gccaagtatg tcagaatttt ttccgcggtg
    37621 ctttagcacc atttcacaaa taccgacaaa atgctttgtt ggatgccact atcgcactaa
    37681 tcaatggcgc atctctgacg ctgaccagca tcggacgtta tttacccggt aatgctcagg
    37741 tcaaaaataa aataaaacgt gttgatcgtc tgctcggtaa tgagtctctt catcacgaca
    37801 tccccctgat tttcagaaac attatttcca tgctgacgag caagctgtca ctgtgtgtca
    37861 tcgccgttga ctggagtgga tacccctctc aggagtatca cgtactccgc gccagcctga
    37921 tttgcgatgg acattcgatt ccattgctga gttggatagt tccttcggaa aaacagcaaa
    37981 atgcaaagat acagcaagcc tttcttaata ctctttccga agcggtaaac ccaaaagcaa
    38041 gggtaatcat cgtcacggat gcagggttcc agaatgcctg gtttcggcac ataaaatcac
    38101 tcggctggga ttttattggc cggatacgag gcaataaaca actgcatctg gcgagaaaag
    38161 gggaatgctg gttcaggcgt caggaattac aggccggtaa caaacctgaa tatctggggc
    38221 cggggacgct ttcccgggcg gaatacgccc gttgcgatgg tcatttttat ttgcacaaaa
    38281 aagagccgaa aggccggaga aataaacgtt cgcgttgcgg tatagccaga ccatcacaga
    38341 taaaagatgc caggtcggcg gcaaaagaac catggttaat attcagcagc acagatgact
    38401 ttaaaccgcg agaaatcatg aagttataca gccgccgaat gcagattgag caaaactcca
    38461 gggatgaaaa aagtgagcgt tttgggttcg gtctgcgagc cagttacagc tgttcggcag
    38521 gaagaatgct ggtactgagc ctgctggcaa cactgagcac aatagtgctg tggcttattg
    38581 gctatcacgc tgaaaatcaa gggttacatc ttagatatca ggccaacagc attaaaacgc
    38641 ggcgggtaat ctcgtatctg aggttagcgg agaatgtctt acgacactcc ccgctaattt
    38701 taaaacgaac ggtgctaagc accattctta atcacctcac cagaacctac cagaatatgg
    38761 tgttggttta ttatcgctga tttatgggga tccctcagcg ccgaagcagg gtctatgtga
    38821 actaaaattc gcttagtgac gagcgcgcac cagctgatac ttacgcgtca gatactccac
    38881 cgggacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
    38941 caacaccagg tgcagacgga agataaacgc cacgccatca agatgttgag aagcgccacc
    39001 gtggaaggtc actacagact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
    39061 atgctgggcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
    39121 caggatcagg atatccgctc cggtagtcgt tgcacgcacg cgtgggctga acagacgacg
    39181 tttcaacaac agcacgccgc caatcagaca cagtacgccg ctggcaccac cagcaaacat
    39241 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcag
    39301 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
    39361 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcacgccatg tgtactgccc
    39421 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
    39481 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
    39541 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
    39601 cagccttttt cagcaaagaa tttaatctgc tcttcttccc agactgcatc cagcgcctgc
    39661 ggcgtatcgt cgcgcgcttc atcggcaatt ttttccgcca ctttgtcgct gtcgattgcg
    39721 gtattcgcca gtttcagcag cagatcaaac agcacggcat aacggctttc acgctgttgc
    39781 agacgcgcgc tcagcaatgc cagaatcggc gcaatatctt tcaaaccctc cacggcttcg
    39841 ctttgcggca gctgcgccag gtactccaga tacagcggca gatggtcggg caattcgcgg
    39901 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca gatccaccat cgcctggccg
    39961 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
    40021 tcgaacagtt cgctgtactg cgcctgggca tcgagcggat ccatcgtcgt taaatcgcgc
    40081 aggaaaatgc ccagcgcatg ggcatcctct tttggcagat ttttcgacgc ggcaatcgcc
    40141 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
    40201 acaatcacga gttcgatcat gggtgcggct ccgttttgct ggttacgtcg atggcatcga
    40261 tacggcggct gttgaacaga ttgaatttgg tatctgaacc gtggcagcca tcaccaaagg
    40321 taaagccgca gccatttttc tccgggaagg cttcccgcgc cagttcacga tgactgctcg
    40381 gcaccacaaa gcgatcttcg tagttggcaa tagccagata acgatacatc tcctgtgcct
    40441 gggcttcggt cagaccgacc tcttccagcg cacgggtatc gactttaccg tcaacggttt
    40501 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcgcgcagt accggtttgg
    40561 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcagg ctttcgacgt
    40621 ctggcagaat gccgttgctg cccagttcac ccgcgtctgc cgcagactga atcggcgaca
    40681 gaggcggcac gtaccagacc atcggcagtg tgcgatattc cggatgcagc ggcagcgcca
    40741 gtttccattc catcgccatt ttataaacgg gcgactgctg cgcagcctca ataacgctca
    40801 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
    40861 catccagctg acgctggtag agatctttct cgttctcggt acttgccgca cgttcaatcg
    40921 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgaac
    40981 atattgtcgg ctggccggct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
    41041 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
    41101 cacggcattt atcctggtcg atcaggacga tgccgtcttc ttcacgcttg taaatcgcac
    41161 cgctcgggca ggtcgccaca catgccgggt tcagacagtg ttcgcacagg cgcggcaaat
    41221 acatcatgaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
    41281 tgtctttcgc cagcttgtca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
    41341 cgattttcgc catccgttcg ccggtaatca gtgaacgcgg acgggcaatc ggctgtgatt
    41401 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
    41461 cgtcgatccc cggcagatgc gggttagcga agattttacc cagcagcatg gcacggttac
    41521 ccatgcgcgg ctgcagtttg ccgttgattt tacggatcca gccgcctttg tatttttcct
    41581 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    41641 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtcactgaa caggtgtggc
    41701 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    41761 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    41821 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccatatgc
    41881 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    41941 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    42001 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    42061 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagctcttt
    42121 ggcatcggct tcactcagcc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    42181 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    42241 tttttccggg ttgccgttgg atttctgccc catcacttct ttcaccgaac gggtgtcgat
    42301 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    42361 cagttgctga cgaccagaga gcgtacgcca cgggatcagc tcgtgaacgt tggtgtaacc
    42421 ggcgttgtaa gaaacgtgtt catcttcaag gccagaccat gtcgggctgg agatgatttt
    42481 gcgcggctgt gcctgaatat cgcggaagcg aatcttctcg tcttctttat tcagcgccag
    42541 atgcgtatgg tcacggccgg taaactcgct cagggcagcc caggctttta cagctacctg
    42601 accattggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtattgag
    42661 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    42721 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    42781 cgggccgata gaggtaaagc gttcgtaagt tgctggataa tcgcgctcta cgaccataat
    42841 gtgcggtgca gttttacctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    42901 cagcggttgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcagt
    42961 ttctttaccc agatgaccaa cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    43021 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    43081 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    43141 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtcacca ccagatccag
    43201 cttgccttcc agaccgttgt cctgccagtc cacttcttcc ggcttcacac cgccctgctg
    43261 ccccaggtct ttgccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    43321 atgaccttta ctggaagaac cgagcaggtt agaacgccag atgaacagat tacgcgggtg
    43381 gtttttaccg ttttccggct gttccgcagc gaaacggata gaaccctctt tcagggattt
    43441 aaccgtatag tccaccggat tcatcccggc tttttctgct tcgcgagcga tagtcagcgg
    43501 gttagtgcct aactgcggcg cagacggcag ccagcccatg cgttctgcac ggacgttaaa
    43561 gtcgatcaag tgtccggtat agcgagattt atccgccatc ggcgacagca gttcttccgc
    43621 agtcacggtt tcataacgcc actggctgga gtggttatag aaataagagg tactgttcat
    43681 gtgacgcgcc ggacgctgcc agtcgagggc aaacgccagc ggctgccagc cggtttgcgg
    43741 acgcagtttt tcctgaccta catagtgcgc ccagccaccg ccgctctgac cgacacagcc
    43801 gcagaaaatc agcatgttga tcagaccacg atagttcatg tccaggtgat accagtggtt
    43861 cagcccagcg ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    43921 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct ctgcccaggc
    43981 aggggtgtaa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    44041 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctacc
    44101 atcagccagt tgcaggcgtt tcaccggcag cttatgcagc agcacgtttt ccagttccac
    44161 tttgttgaag tgttccgtgc catcgccacc aaagtacggg aagcccacct cagcgatctc
    44221 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    44281 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    44341 aaccatttca ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    44401 cagtgcatcc accagatcag cagcgcgcag catacgaccc gcagcgtagt aaccgtcgcg
    44461 ctcttccagc atcaccagca tcggcatgtc ggtgtagcgg cgcacatagt cggtgaaata
    44521 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tgacccattg ccagcgccat
    44581 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    44641 gtagtctggt gtgacggcaa cagttttggt ccctttgtaa cgcacttcag taaagaagtg
    44701 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    44761 gtaccagtca gcagattccg gtacgtcagt ttgctcaccc caggtttgcg gagacgcagg
    44821 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    44881 gcgcgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga agccagcgac
    44941 acggtccggg ccgtaatttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    45001 ttcctgccag gaagaacgaa caaaaccacc gcgtccacgc gcctgcttaa agcttttcgc
    45061 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    45121 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttagc
    45181 gctgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    45241 caggtccggg cgagtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    45301 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    45361 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    45421 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    45481 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    45541 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    45601 gagtgatgga gatgaaaaat aaagtaaatt cctggaatac ccctttagga taattaacat
    45661 ttatagcata acagactgta aattaagacg ttttacacct gtagttattt aaaccattaa
    45721 gatttttatg cagatgggta ttaaggagta ttccccatgg gtaacatatt aatgactctt
    45781 aatgaaatcc agcgaaataa gccatacaac attagtatgt tgtaactaaa tgacgtataa
    45841 aaaaagcgcg acatcatgcc gcgcaaagga taatcaaata ttacgattta ttttttagaa
    45901 tgccgaccat ataccgccca ggtaatcacc acgcaggcga tatagaaaat caaaaatact
    45961 ttcattgcgc cgactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    46021 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgtcggtt
    46081 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    46141 agtttacgga agatcacgga aatcatctgg aaagtggaac cactacccag cccagcggtc
    46201 aggaacagcg ccaggaagac tgcgaagaac gccatgaagc ttccgccctg tccgtcagtc
    46261 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca ggataaagtt caccagcgtg
    46321 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    46381 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    46441 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    46501 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    46561 gaggtggcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aagtaacggc
    46621 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    46681 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    46741 acgctgacgc ccatgttgcc cagaccccca ttcagaccca gcgcgccacc ctgcttctgt
    46801 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    46861 cacagcagag aaatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    46921 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    46981 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    47041 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    47101 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    47161 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    47221 ggtcgccaat ctgtaatgac agctccagta gccctttcgg gggcggatga gtgactcata
    47281 gacacctctg atactcgttt cgatgtctgc caccttagtg tctgtagcta aaggcaattt
    47341 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tatccatagt
    47401 gagtacagtg atttcataaa aattatgaga tttttcacgg tactgtaaaa tccctaccct
    47461 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc gggtatgggt
    47521 atacttcagc caatagccga gaatactgcc attccagaat gtatcgtcac attcattaag
    47581 gttattgctc atttaaagcc tgaaggaaga ggtttacatg cttaaacgtt gtctctctcc
    47641 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    47701 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    47761 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcagcagtgc cattaagcga
    47821 gaaagacaag cccttaatta aagagatgga acaaacagca tttagcgccg agttgactcg
    47881 agcagcagaa cgagacgggc aactggcgca attacagggt ttacaagatt actggcgtaa
    47941 tgaactgatc cctgcactga tacgtgcaca aaaccgcgaa acggtgtcag cggatgtcag
    48001 ccagtttgtt gccgggcttg atcagctggt atctggtttt gatcgcacca cggaaatgcg
    48061 cattgagaca gtggtgctgg tccatcgggt aatggcggta tttatggcac ttttactggt
    48121 attcactatt atctggttgc gggcgagatt gctacaaccg tggcggcaac tgctggcaat
    48181 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacaccagcg ggcgcaacga
    48241 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    48301 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    48361 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    48421 gtcacctgta ctcaacggat tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    48481 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    48541 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggcg atcgcggcac
    48601 aaccctgaag tggcggctgg ctgacactca tacgcagtac ggtattttgc tggcgaccct
    48661 gccgcagggg cgtcatctta gccatgatca acaacaactg gtggataccc tggttgaaca
    48721 actcaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    48781 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    48841 catgaagatg caggtgagtt gcttacagat gcagggcgat gcgctgccag aaagcagccg
    48901 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggtgcagt tgcgtgaatt
    48961 gctcaccaca ttccgtttgc agctcaccga gcctggatta cgtccggcgc tggaggcgag
    49021 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    49081 tcgtctggtg ccttcgcatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    49141 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    49201 tcaggtcaaa ctgaccgtcc aggataacgg ctgcggcgtg cctgaaaatg ccatccgcag
    49261 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    49321 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    49381 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    49441 tcgacgatca cccgatgttg cgaactggcg taaaacagct tatcagtatg gcaccagata
    49501 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gagtctcttg
    49561 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    49621 ataaactgcg cgaaaagtcc ctctccgggc gcattgtggt attcagcgtc tctaaccatg
    49681 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    49741 aaccggaaga tctgctgaaa gcgttacatc aggcagctgc tggcgaaatg gtattaagcg
    49801 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    49861 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctaattgcc cagggtttgc
    49921 cgaacaagat gattgcccgc cgcctggaca tcaccgaaag cacagtaaaa gttcacgtca
    49981 agcacatgct gaagaaaatg aagctaaagt ctcgcgtgga agctgcggta tgggtgcatc
    50041 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcgtcgaac
    50101 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    50161 accaccactg acaatcgcca gtgattgctt gcgccttccc cgttttgcca gtcaggcatg
    50221 atcagcgtcc agccctcctc actgttggct tgtgcgcccg gcgtcaaact taatatctgc
    50281 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    50341 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgtcaacgtt
    50401 tttcgctgtc ggtactcaag agtcggaaga ttatttcgct gcggattgtc atagcgacta
    50461 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    50521 ccaaagcggt agttaagatt cagcccgagg ttattttgat tctcgccact ttcaccctgt
    50581 ttatgctggg ccgtcacagt gactaagggc acaggggtgt aatttaatcc cagactcaac
    50641 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat cgacacgatc accaaaatac
    50701 tgttctacgc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgggctgtc
    50761 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    50821 aacggctgat aaaagtttgc cgatagtcgc aaatattcgc cccacgcttc agcgccaaag
    50881 cccgctcgct gaagattttc gtccagcaag ttgtcataaa aagtgttata acccaccagc
    50941 cagctgccgc gcgcccagcg ttgcccaacg tccacattgc tcaccagccc atcatcctgc
    51001 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    51061 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    51121 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    51181 cggacctttc ccaatgcgaa agcttttgcc tgctcgccag tatcaagccc gttatcattc
    51241 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    51301 tcaggtgcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    51361 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ccgccagcaa gagtaaataa
    51421 aacggaataa tgcgaggaat gaaacggctc aaaacgtcgg taacttccgg taaaaatgaa
    51481 aaaccatagc cttaaagaat aacgattact ggcacctttt gtcgtatttt ctcgcgcttt
    51541 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    51601 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatcgt gatcgttgcc
    51661 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    51721 cgagagcagg agaacaatct ggatctgcgt ctgttcctga tgtctgatgc ggtaacagcc
    51781 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc aacaaatgct ggagatcctt
    51841 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagt
    51901 acacttcctc tgattgatgg ggtggaaatc ggtactcttg ttgaactggc gcaatggacg
    51961 ctgtcagccg ataaagtgct cacattttaa tgtttgaggg ggcgtcggac gccccactat
    52021 gaggaaagtt tactcaggct aatcccggcc gtaacacacc atccggataa ttctccgcca
    52081 gcagtttttt taccgatacc agcaattcat tcagcccatc gtcctgcatt cccagtttga
    52141 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    52201 ccgcaatcaa cggcgctatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    52261 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    52321 aggttggcag ataacagccg gtaatcatct cgcgcttcca cagcagggtt agttcctgct
    52381 ccagcgtctc ttctggcagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    52441 caagcattcg tcccggctgc tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    52501 cgcgatgcca tccaaccagt gtaccggtgc acgactcggt aaactccagt gccggattcc
    52561 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    52621 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    52681 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    52741 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt tactgctcat
    52801 tttgaaacca ctaacgggac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    52861 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    52921 ttggcatatt catgcttcac agcagcccag gccactttat gcgctgtttc ttcgcgactg
    52981 gcgtcatcac gccaatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    53041 ttatagatat cctgggcatg agacggaagc acgtgcttta cgctttccgg cagatcgctt
    53101 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    53161 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctcttcttaa gaagtgctaa
    53221 aacaaacatt gtctaaacgt attacttatt tttatgccca aatgagtaaa aaatcgacag
    53281 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    53341 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    53401 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggcgctggt
    53461 agtgctgttc ctgtggggaa gcagccagac actaccagtg gtcattgcca tcaatcttct
    53521 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    53581 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    53641 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    53701 tacgctctat tcaatcatta tgattgttac aggtgggctg gttggctttt cattattgtt
    53761 gggcggacgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    53821 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggcgctgc ctgcggcgaa
    53881 tttttcaaca ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    53941 tttgctgatc cagaccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    54001 tgatgatgac gacccgcacc acggtaaacc gtctgcccat agcagcctgt ggcatgccat
    54061 ctggttaatt gtccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagcccgct
    54121 ggagacattg ctcgacagca tgaatgcgcc tgtcgccttt actggcttcc tggtggctct
    54181 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaata accaggtcca
    54241 gcgcgcgatg aatctgttct ttggttcagt gttggcaacc atttcgctta ccgtacctgt
    54301 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    54361 aatggtggtg atggtggcct ctttagtact gtgccatatc tccttctcca ccggacgaac
    54421 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    54481 tgcctgacag ttatatctcc tccgatgacg gaaatcgggg gagataatat aaattcctgc
    54541 ttattgtatt tcttcatact acctggacag gtcgttaatc agaattgcga gcctgtacca
    54601 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc ttcaatgcaa
    54661 acatttcagg gatgaagtga agatgaattg cgacgtagta tccacaccaa gccggcgtta
    54721 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    54781 aaaacccccg cacgttgaga ggtaaaacct gacaacagaa cgggggctaa tcttgaacca
    54841 tcaacaattc aagaatagcc gcaaaatgtt gtcattacaa caaggcggct atatgacgct
    54901 cgcgcagttt gccatgattt tctggcacga cctggcagca ccgatcctgg cgggaattat
    54961 taccgcagcg attgtcggct ggtggcgtaa ccggaagtaa ggtgaaaacg ggcgtcaggc
    55021 tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg taacagcctg cggccttttt
    55081 tgtatccggc ccccataaaa aaagtcttaa cgcagaacgc taagacttta tttttcaaac
    55141 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    55201 tcatttgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    55261 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    55321 cggctcttgc cagctcagcc acctgagcac ggcgaccgcc ggaagctgcg ccaaatggat
    55381 cgcgacattg cagtgcgtgg gtaacgtcga aaatcaccgg cgaattacca gacactttct
    55441 tcatgatgct gaagcccagc atatcaacaa ccaggttgtc atagccgaag ttagcaccgc
    55501 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    55561 tctgtcccgg gctgacaaac tgcggtttct tgacgttaat taccgcaccg gttttcgcca
    55621 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    55681 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    55741 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    55801 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    55861 gtttctgggt cacggttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    55921 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    55981 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    56041 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    56101 cgggtcttcc ggacactgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    56161 atccagttgc gcataaatca acccgcgatc gcgaatttca tagggatctt ccgggttgaa
    56221 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    56281 agctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    56341 ttcataaaac agttccgccg acgggctgat atttcccttt aaccacacat ccagcatatg
    56401 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    56461 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    56521 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    56581 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaaccccca
    56641 ttcgccgtaa aacagcgcga ttaatttttc tagctgctct tctaatggca gaagctggct
    56701 aatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    56761 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    56821 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    56881 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    56941 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacaatat aaatgataac
    57001 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggctgga tgtgcgcttt
    57061 agccatcaac gcaatgccgc ttaacaataa cagcgtatcg acaaccggcg gcacaatgcg
    57121 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggtcaa
    57181 tagcccaaca gaaagcgcga tactaatgag atgaacgcta agcaacgtag aaaaacttgt
    57241 cattgataat agcggccaag cgttacgcgc tcgttatccc catagtcacg gcaggtttcg
    57301 acgtcatgat accccgcgaa gataaatgcc tgtcgcaccg cttcaccctg ctgccagcca
    57361 tgctccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    57421 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    57481 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    57541 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    57601 atagccagat gctgggcatt acgttgcgcc agagagacag catctggcat acgatctaca
    57661 gcggtaattt cgcagtccgg acgctcgctg gccagcgcca gcgcaatcgc cccggtaccc
    57721 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    57781 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    57841 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgcgtcagt
    57901 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    57961 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    58021 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    58081 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    58141 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    58201 cggtaacgcg cccctgcggg aagttgtaag tacggttacg gtcgctgcga tcgccactcc
    58261 ccagcaggtt acgacgggta gacgcttcgg cctgttgccg ttttgccatt tcagcagcgt
    58321 ggatgcgagc accgagtaca gaaagtgctt tagctttgtt tttatgttgt gaacgttcgt
    58381 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    58441 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    58501 ggttgatgtc cggcagttct gcatcaggca gttctggcat taccgcaacg gtacaagcag
    58561 aagtatgaat acgaccctgc gattccgtag caggaacacg ctgcacgcga tgaccgccag
    58621 attcgaattt caaacgacca tatacaccat caccgctaat tttggcgatg atctctttat
    58681 aaccaccatg ttcaccctcg ctggcgctca tgatttctac acgccagcgg cgggcttcgg
    58741 cgtaacggct gtacatacgg aacagatcgc cagcgaacag cgccgcttcg tcgccgccgg
    58801 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaaca
    58861 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    58921 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    58981 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcacgag
    59041 ataatgcgcg aaaacgttcc tggtcagcga tggtttgcgc atcacccagc agcgcctgaa
    59101 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    59161 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    59221 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg attttgttgg
    59281 tgcatggatc aggcggttag tcagtttcca tgccagatcc tgcataatgg tttgcgcatc
    59341 accgccctgt tcaagggccg ctaacgcttt agcagttaac tcatcacgaa cctgctctgc
    59401 ctggctacga taatcgcgaa tagtttcgct ggcgctttgt gctcgaagcc atgccataaa
    59461 ttcactggcc tcctgggcga cgatagtttc cgcctccaca gcagcagctt tccgttgcgc
    59521 caggttgtgc gaaataatgc tttgcagatc gtcaacgcta taaagataag cattcgccag
    59581 tttaccaact tccggctcaa catcgcgcgg aacggcaata tccaccagca gcattggttg
    59641 attgcgacgg ctttttaatg cgcgctccac catgccttta ccgataatcg gcaacgggct
    59701 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatat cgctcagggc
    59761 aatcacttcc gcgccaacct catctgccag aatttgggca cgttcgcgag tgcgattggc
    59821 gataatcatc ttctgtactt tgtgttcacg cagatgacgc gccaccagct cgatggtttc
    59881 gcccgcgcca accagcagta ccgtcaccgt aaagagcgat tcaaagatct gccgcgccag
    59941 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    60001 gcgtttcgcc acagagaaag atttctgaaa catgcgttcc agttcgctgg ccttcatatg
    60061 acctttttgc gaatcggcaa acgctttttt aacctgaccg aggatctgcg gctcgccaag
    60121 gacgagagaa tcaagcccac tggcaacgcg cattaaatga ctgaccgcat cgttatcctg
    60181 atgccagtag aggctattac gcagatcgtc ttcgttgaga ttatggtaat cacacaacca
    60241 gcgaataagc gcctcttgca ggtcatcccg ttcttcaacg ctgagataaa gttctgtgcg
    60301 gttacacgtc gacagcacca caccgccctg caccatcggc tgcgcgagca gactgtcaag
    60361 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    60421 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    60481 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    60541 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacgtg aacatagacg
    60601 atggctaacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    60661 attattatgc ccctgcccga ttttcgcttt atccgcctgc taccgctagc agccctcgtg
    60721 ctcactgcct gttctgttac cacacccaaa ggcccaggca aaagcccgga ttcgccacaa
    60781 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcaaactcg cggcgcgttc
    60841 gcttatattt ctgaccaaca aaaagtgtac gcccgtttct tctggcagca aactggccag
    60901 gatcgctacc gtctgctgct gactaaccca ttgggcagca cggaactgga gctgaatgct
    60961 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttatactgc cgatgacgcc
    61021 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    61081 attttaggtt taccgggtga tgcgactgac tacaaacttg acgaccagta ccgcctgagt
    61141 gaaatcacct acagccagaa tggcaaaaac tggaaagttg tttacggtgg ttatgacaca
    61201 aaaactcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    61261 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    61321 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    61381 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    61441 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    61501 cgaggttgtt gatgaaaact gcggcagaca gcgggcgtct ttcgacggga agcggtgcga
    61561 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    61621 ccgcgacggt tctggtggcg ttaaatcatc tctggcaatg cggactaagc atggatgagc
    61681 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgt ggtcatgccg
    61741 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    61801 tggtggcgca ccctggtgta agtattccga caccggtgat ttttaaagat cctgaactcc
    61861 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    61921 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    61981 aatacgcccc gtcgcgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    62041 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    62101 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    62161 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    62221 gaatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    62281 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gaactagcac
    62341 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    62401 acggcgaagt cagtgtacaa attaatgaaa atgtacgcgg tggtgatatt ttcatcatcc
    62461 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    62521 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    62581 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    62641 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    62701 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    62761 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    62821 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    62881 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    62941 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagctctga
    63001 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    63061 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    63121 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    63181 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    63241 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    63301 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    63361 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgcttgc
    63421 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    63481 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    63541 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctgacggg ggggtcacgc
    63601 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    63661 tttggactgg caggactgct ggttgcgacc ttgctgtcgg ggatcttttt gattctgatg
    63721 gggctggcac gctttgggcg cctgattgag tatattccgg tttccgtcac cttaggtttc
    63781 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    63841 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    63901 accattaatg tgggtgatgc agccattggc attgtgacgc ttggcattct ggttttctgg
    63961 ccgcgtctgg gcatacgttt acccggccac cttccggcct tgctggctgg ttgcgcggtg
    64021 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    64081 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    64141 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg cacactgctg
    64201 cctgcggcat tctcaatggc aatgctcggc gcaatcgaat ctctgctctg cgccgtggtg
    64261 ctggatggta tgaccgggac gaaacacaag gcgaacagcg aactggttgg acagggattg
    64321 gggaatatta tcgctccgtt ctttggtggt attaccgcta cagctgccat cgcgcgttct
    64381 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    64441 gttattcttg ccctgctggt actggcaccg ctgctctcct ggctgccgct ttccgccatg
    64501 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    64561 ctgcgtcatg caccgaaaga tgacatcatc gtcatgctgc tgtgcatgtc gctgaccgtg
    64621 ctgttcgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    64681 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagacgttcc agacgatgtc
    64741 ctggttctgc gcgttattgg cccgctgttt tttgctgccg ctgaaggctt attcacggac
    64801 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    64861 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    64921 gaactgcgcg tgtgcaacct ggaattccag ccactgcgca ctatggctcg cgcaggcatt
    64981 caaccgatcc cgggacgcct cgcattcttc ccgaatcgtc gcgcggcgat ggcggattta
    65041 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    65101 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtggcgaa cccaccagta
    65161 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatcgcacc
    65221 gacgaaaata cttagcacag caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    65281 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    65341 cacgttaccg agtaacgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    65401 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtaggtta
    65461 tgtgcgggtg tgatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    65521 gaacaccttg actttctaat agtccactca ggcaataatt gcattatctg catttgatac
    65581 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    65641 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    65701 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgattgcg
    65761 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggagg
    65821 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    65881 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    65941 ggatctgcct cctggcgtcg ccaaatttaa gttgggtggc ggtcatggtg gtcacaatgg
    66001 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    66061 aatcggtcat ccgggcgata aaaataaagt tgtcggcttt gtgttaggca aaccgcctgt
    66121 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    66181 gtttacagat ggcttgacca aagcgacgaa ccgattgcac gcctttaaag cgcaataagt
    66241 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    66301 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    66361 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    66421 accaaagccg gtattgaagc ggccaacttt ccattctgca ccatcgagcc gaacactggc
    66481 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcagcgt
    66541 acacttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    66601 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    66661 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggcg
    66721 gatgatattg aagttatcaa caccgaactg gcgctggcgg atctcgacac ctgtgaacgt
    66781 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agccgagctg
    66841 gcggtactgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg tgcgctggat
    66901 ttaagcgctg aagagaaagc ggctgttcgt tacctgagct tcctgacgct aaaaccaaca
    66961 atgtacatcg ccaacgtcaa cgaagacggt tttgaaaaca acccgtatct tgaccaggtg
    67021 cgtgaaatcg cagcgaaaga aggttctgtt gtagttccgg tttgtgctgc tgttgaagca
    67081 gacattgccg aactggacga cgaagagcgt gacgagttta tgcaggagct tgggctggaa
    67141 gagccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    67201 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggtgc aaccgcgccg
    67261 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    67321 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    67381 cgtgcagaag gcaaagatta catcgttaaa gatggcgatg tgatgaactt cctgttcaac
    67441 gtctaattcc gctaatacta ccctggcggt taataacgag ccattatgtg agttgcacgt
    67501 aatggctttt ttacgcctgc tacgcgtctt ctttcaattc tgagttacca aaactcataa
    67561 tcagactcct gacaatatta acaatgcagc ttttatgtga taacaatcac agtgcattaa
    67621 ccgttcaaga gtttgtcatg aaaaaaacat ttaccttcag cctgttagca attgtatttg
    67681 caagcctgtt gagtgcttgt gttccccacc atcatcaccg ccacagcgat ggccctcgtg
    67741 gtcctgcgcc ctcaggtaag ccaatgccac cttcaaatgg tggaccaaat cacggcagtc
    67801 atggtcgcta ttaattagca attcatagcg ggaaagtaca tactctcccg ctatccacgg
    67861 cgcttaaact gccctgcatc aatgccatgt tgcttcattt tccgccacag cgtagtgcgg
    67921 ccgatcccaa gtaaagccga catttcctga atgcgaccgc ctgtgacctg ggctgcgtta
    67981 ataattgcct ctttttcaac ttccgcaaat gacagactgg tggaaaggcg ggtggcgctg
    68041 acatcatctg tcgcctgctc ggtaaacaga tgttccggca aatcactgac gcgaatgcgc
    68101 ccattatcac tactcagagc aagattctcg atgacgctgt aaagttcaaa atcgttgcca
    68161 ggccatgcac aggaaaccag gcgagcgagg gcatcgtcat caattttcag ccgcgtagag
    68221 aagcgttttt caaggctgcg taatttgtta ttcaccagcg ccggaatgct gccacgccgc
    68281 atacgcagtg gcgggatggt aatttcaaat gcatgcagcg cgtaatacag ctggcgacta
    68341 aaacgatttt gttccaccag cattgcgagg tccgcggtcg ttgtggcaat cactttgaca
    68401 tcaatgggta ttaaacgccg cgcatccagt cgcgtgataa ccccctgctt gataacctga
    68461 agcaaagcag actgtaactc caccgccaga tattcaatct tttcaagaaa cagcgtgccg
    68521 ccgtgcgcca gttccagccg actcagacgg ccattttcat tatccgtgcg atcgccacca
    68581 ataaattctt ccgccagcgc cgcatcacca tataattcac aattgacggc gatataaggc
    68641 cccgctgcac gctcgctttc attatgaatt gcctgactta gcagtgcctt gcccacgccc
    68701 tcttctccac aaagcaggac aggaaagcta ctgcgtgccg cctggcgacc aaaatgaatc
    68761 aagcggcggg tttgcggatc gtcctgtggc atatgagcga aggtatggct gacttttcct
    68821 aattgactgg tcattaactg ccgcatctgt tccacaggat ggagcaataa aataaagctg
    68881 gttccctgcg tttctattat cggtttaagg gttatcaccg catcaataaa ctgatgctga
    68941 ctttcaaagg tggcttctac gtgtttgagc ggatgtgcct gttttattgc ttgttgcaat
    69001 acggcgggta acgttaagag ttcagtgatt gcccttccct gacttgccgt cgcgtcaagg
    69061 cgcaagaccc gcgccgcctg ggcattgata aattgcaaat taccctgctc gtcccagcta
    69121 atcacgccat catccatact ttctaacagg gcattaagtt gatttaaatg acggttagtt
    69181 tcagcgagca aactgtccgt cagcagtaaa tttccgacct cgcgtgcgat tgccaacgtc
    69241 aacggcaaat cagctgcggt agtttgctca accggacacg ccagcgctat tgttcccgtc
    69301 aatcggccct tgctgtcaaa caacggcgtt gcgcaaaagg cccagttcca gagtgcctgt
    69361 ttgaaatgtt gatcggccat cgttttcacg gcctgaccag agatagccgc taacgatagc
    69421 gcacaagtac caataattcc ctcggcgcaa tacgtgccgt cattgaatcc cagtgtactt
    69481 agttgctgca aggtttgcgg atcgccatta cggctgagaa tgcaggcggt ttcatcgagg
    69541 ataagcaacg cacactctcg cggtgccata tattcccagg catcttccag cgcagcctgc
    69601 ccgagcgtca gcatcgcttt cttacgccga taaatagaag caaatgtcac gccctgggcc
    69661 tggtgtggta cgttccatgt ctcccgtttc atcagcttat tgcatctctc ccaggaggtt
    69721 gcaattaagg gagatatgcc ccgaccatcg ttgttaaaag cgccactcat atcccgcatc
    69781 ccttatgaca aaaggtgttc cggctcgcaa ttttaagtat taatacacgt aaaagtttca
    69841 atatggaaca ttcattaaag catatgtttc actatgaaac ctaatttggg ctaattttct
    69901 tttcccttgc ctgatgcaca atggattcat cggcagtcag tggtcgccgt gtcgttgaac
    69961 atcatccatg ccctaccgta attgctggag caaaataatg aaaaaattga tcaatgatgt
    70021 gcaagacgta ctggacgaac aactggcagg actagcgaag gcgcatccat cgctgacact
    70081 gcatcaggat ccggtgtatg tcacccgagc tgatgccccc gttgcaggga aagtcgccct
    70141 gctgtcgggt ggcggcagcg gacacgagcc gatgcactgt ggctatatcg gtcaggggat
    70201 gctttctggg gcctgtccgg gcgaaatttt cacctcaccg acgcccgata aaatctttga
    70261 atgcgccatg caaattgatg gcggcgaagg tgtactgttg attatcaaaa attacaccgg
    70321 cgatattctt aacttcgaaa cagcaaccga gttactgcac gatagcggcg taaaagtgac
    70381 cactgtggtc attgatgacg acgttgcagt aaaagacagt ctttataccg ccgggcggcg
    70441 cggcgttgcc aacaccgtat taattgaaaa actcgtaggt gcagcggcgg agcgtggcga
    70501 ctcactggac gcctgtgcgg aactggggcg taagttgaat aatcaaggcc attcaatagg
    70561 tatcgctctc ggtgcctgta ccgttcctgc cgcgggcaaa ccttctttta ccctggcgga
    70621 taatgagatg gagtttggcg tcggcattca tagtgagcca ggtattgacc gccgcccctt
    70681 ctcttccctt gatcaaaccg tcgatgaaat gttcgacacc ctgctggaaa atggctcata
    70741 ccatcgcact ttgcgtttct gggattatca acaaggcagc tggcaggaag aaccacaaac
    70801 caaacaaccg ctccagtctg gcgatcgggt gattgcgctg gttaacaatc ttggcgcaac
    70861 tccgctttct gagctgtacg gcgtctataa ccgcctgacc acacgttgcc agcaagcggg
    70921 attgactatc gaacgtaatt taattggcgc gtactgcacc tcactggata tgaccggttt
    70981 ctcaatcacc ttactgaaag ttgatgacga aacgctggca ttctgggacg cccctgtcca
    71041 caccccggcc cttaactggg gtaaataagg agaaagcaat gtcactgagc agaactcaaa
    71101 ttgttaactg gctcactcgt tgtggcgata ttttcagcac cgagagcgag tatcttactg
    71161 gactggatcg cgagattggc gacgctgacc acgggctaaa tatgaatcga ggctttagca
    71221 aagtggtaga aaaactccct gctatcgcag ataaagatat cggtttcatt ctcaagaata
    71281 ccggcatgac actactttcc agcgtcggag gtgccagtgg tccgctgttc ggtaccttct
    71341 ttatccgcgc cgcacagaca acccaggcac ggcaaagcct gacgctggaa gagctttatc
    71401 agatgttccg cgatggcgcg gacggcgtaa tcagtcgcgg gaaagccgaa cctggcgata
    71461 aaaccatgtg tgatgtgtgg gtgccggtgg tggaatcgtt acgtcagtcc tgcgagcaaa
    71521 atctccctgt tccggcggcg ctcgacgctg ccagtagcat cgccgaatcc gctgcacaaa
    71581 gtacgattac gatgcaagcc cgcaaaggcc gcgccagtta tctcggtgaa cgcagtattg
    71641 gtcaccagga tcccggcgcg acctcggtga tgtttatgat gcaaatgttg gcgttagccg
    71701 caaaagagta aggaattggt gatggtaaac ctggtcatag tttcacatag cagccgactg
    71761 ggagaaggtg tcggtgaatt agcccgtcag atgttaatga gtgataattg taaaatcgcc
    71821 attgccgcgg gaattgacga tccacaaaat cccattggta ccgatgccgt caaagtgatg
    71881 gaggccatcg aatctgttgc cgatgccgac catgtgctgg tcatgatgga tatgggtagc
    71941 gcattattga gtgctgaaac tgcgctggaa ttgctggctc ccgagatcgc cgcaaaagta
    72001 cgtttgtgtg ctgcgccgtt ggtcgaaggt acactggcag caacggtcag cgcggcctcg
    72061 ggggcggata tcgacaaagt gatctttgac gccatgcatg cgctggaagc caaacgtgaa
    72121 caactgggtt taccgtcctc cgacactgaa atctctgaca catgtcctcc gtacgatgaa
    72181 gaagcccgtt ctctgtcggt ggtcataaaa aaccgtaacg gcctgcatgt acgtccggcc
    72241 tcccggctgg tttatacctt atcgacattt aatgccgata tgttgctgga gaaaaacggc
    72301 aaatgcgtta caccggagag tattaaccag attgcgttac tacaagttcg ctataacgat
    72361 acgctgcgcc tgattgcgaa aggaccagaa gctgaagagg cactgatcgc cttccgtcag
    72421 ctggctgaag ataactttgg tgaaacggag gaagttgctc cacctactct gcgtcccgtt
    72481 ccgcctgttt cgggtaaagc cttttattat caacctgttt tatgtacggt acaggcaaaa
    72541 tcaaccctga ccgtggaaga agaacaagaa cgattacgcc aggccattga cttcacgtta
    72601 ttagatctga tgacgttaac agcgaaagca gaaaccagcg ggcttgacga tattgccgca
    72661 atcttttctg gtcaccatac actgttagat gatccggaac tgctggcggc ggcaagcgaa
    72721 ctccttcagc atgaacattg cacggcagaa tatgcctggc agcaagttct taaagaactt
    72781 agccagcaat accaacagct ggatgatgaa tatctacagg ctcgatatat cgatgtggac
    72841 gatcttctgc atcgcaccct ggtccacctg acccaaacga aagaagaact cccgcagttt
    72901 aactcgccaa ctattctact ggcggagaac atttatccct ccacagtact gcaactggat
    72961 ccagcggttg taaaaggtat ctgccttagc gccggaagtc cgctatccca cagtgcccta
    73021 atcgcccgtg aactgggaat tggctggatt tgccagcagg gtgagaaact gtatgcgata
    73081 caaccagaag aaacgctaac gctggacgtt aaaacgcaac gtttcaaccg tcagggttaa
    73141 accaccgttc ggatggcatc attctgatgt catccggcaa tttactctgc actgatgttt
    73201 tttaactacc tgttatattt ttcaccaaat tatgatgatt tttcttcgct aatcgcgaac
    73261 ccaacgtcgc tggctgacta ataaacagat gttattttat cactctattt cgccgttatt
    73321 tatcccttta attccctttt ctaaaatgtc tgtcagttcg cagaatgtga tttcgatcat
    73381 gcagctagtg cgatcctgaa ctaaggtttt ctgatacttg aataccgttt tattcggttt
    73441 cgccaaagga gaatgactga tgaaatcccc cgcaccttct cgcccgcaaa aaatggcgtt
    73501 aattccagcc tgcatctttt tgtgtttcgc tgcgctatcg gtgcaggcag aagaaacatc
    73561 agtaacacca caaccgcctg atattttatt agggccgctg tttaatgatg tgcaaaacgc
    73621 caaacttttt ccggaccaaa aaatctttgc cgatgccgtg ccgaacagcg atccgctgat
    73681 gatccttgct gattatcgga tgcagcaaaa ccagagcgga tttgatctac gccatttcgt
    73741 taacgtcaat ttcaccctgc cgaaagaggg cgagaaatat gttccgccag aggggcaatc
    73801 actgcgcgaa catattgacg gactttggcc ggtattaacg cgttctaccg aaaacaccga
    73861 aaaatgggat tctctattac cgctgccaaa accttatgtc gtgccaggcg gacgttttcg
    73921 cgaggtatat tactgggaca gttacttcac catgttagga cttgccgaaa gcggtcactg
    73981 ggataaagtc gcggatatgg tggccaattt tgctcatgaa atagacactt acggtcatat
    74041 tcccaacggc aaccgcagtt actatttaag ccgctcgcag ccgcccttct ttgccctgat
    74101 ggtagagtta ctggcgcagc atgaaggcga cgccgcgttg aaacaatacc tgccgcaaat
    74161 gcaaaaagaa tatgcttact ggatggacgg tgttgaaaac ctgcaagccg gacaacagga
    74221 aaaacgcgtt gtaaaacttc aggatggtac ccttctcaac cgctactggg acgatcgcga
    74281 tacgccacga ccagagtcat gggtggaaga tattgccact gccaaaagca atccgaatcg
    74341 acctgccact gaaatttacc gcgacctgcg ctctgccgct gcgtctggct gggatttcag
    74401 ctcgcgctgg atggacaatc cgcagcagtt aaacacctta cgcaccacca gcatcgtacc
    74461 ggtcgatctg aacagcttga tgttcaaaat ggaaaaaatc ctctcccgcg ccagcaaagc
    74521 tgccggagat aacgcgatgg caaaccagta cgaaacgctg gcaaatgccc gtcaaaaagg
    74581 gatcgaaaaa tacctgtgga acgatcaaca aggctggtat gccgattacg acctgaaaag
    74641 tcataaagtg cgcaatcagt taaccgcggc cgccctgttc ccgctgtacg tcaatgcggc
    74701 agcgaaagat cgcgccagca aaatggcgac ggcgacgaaa acacatctgc tgcaaccagg
    74761 cggcctgaac accacgtcgg tgaaaagtgg gcaacaatgg gatgcgccaa acggctgggc
    74821 accgttgcag tgggtcgcga ctgaaggatt acaaaactac gggcaaaaag aggtagcgat
    74881 ggacattagc tggcacttcc tgaccaatgt tcagcacacc tatgaccggg agaaaaagct
    74941 ggtggaaaaa tatgatgtca gcaccaccgg aacagggggc ggcggtggcg aatatccatt
    75001 acaggatggc tttggctgga ccaatggcgt gacgctgaaa atgctggatt tgatctgccc
    75061 gaaagagcaa ccgtgtgaca atgttccggc gacgcgtcca ctcagtgaat caacaacaca
    75121 gccgctaaaa caaaaagagg cggaacctac gccttaactt tactgtatat cgagcaatgc
    75181 cattacgtgc ataaaattgg cattgctctt tcttcatcac gaaataccaa ataccactct
    75241 ttctacggtc tatgccttta ttttctttta ttagagaaga tttta
//
LOCUS       DAZGJT010000007        70839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZGJT010000007 DAZGJT010000000
VERSION     DAZGJT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.007"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..457)
                     /locus_tag="ACHKFK_000516"
     CDS             complement(<1..457)
                     /locus_tag="ACHKFK_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9050937.1"
                     /translation="MKVSTLGIDLAKNVFQLHGVDHEGHTILRKKLTRAKFVQFVIQL
                     EPCLIGMEACSSSHYFARLFTRYGHEVKLIPPQYVKPYVKTNKTDAADAEAICEAVTR
                     PNMRFVQIKTEEQQAVLALHTERGILIRERIACANSLRATLAEFGITIAA"
     gene            complement(689..1636)
                     /gene="dusC"
                     /locus_tag="ACHKFK_000517"
     CDS             complement(689..1636)
                     /gene="dusC"
                     /locus_tag="ACHKFK_000517"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO9050938.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1875..2273
                     /gene="yohJ"
                     /locus_tag="ACHKFK_000518"
     CDS             1875..2273
                     /gene="yohJ"
                     /locus_tag="ACHKFK_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO9050939.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            2270..2965
                     /gene="yohK"
                     /locus_tag="ACHKFK_000519"
     CDS             2270..2965
                     /gene="yohK"
                     /locus_tag="ACHKFK_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO9050940.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            3095..3979
                     /gene="cdd"
                     /locus_tag="ACHKFK_000520"
     CDS             3095..3979
                     /gene="cdd"
                     /locus_tag="ACHKFK_000520"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO9050941.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKVDAPLIQWDAT
                     SATLKALGCHNIDRVLLA"
     gene            4129..4848
                     /gene="sanA"
                     /locus_tag="ACHKFK_000521"
     CDS             4129..4848
                     /gene="sanA"
                     /locus_tag="ACHKFK_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO9050942.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4851..5090
                     /gene="yeiS"
                     /locus_tag="ACHKFK_000522"
     CDS             4851..5090
                     /gene="yeiS"
                     /locus_tag="ACHKFK_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO9050943.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            5284..6522
                     /gene="preT"
                     /locus_tag="ACHKFK_000523"
     CDS             5284..6522
                     /gene="preT"
                     /locus_tag="ACHKFK_000523"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO9050944.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYLPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIETQHYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAGAIHHYLEGACSC"
     gene            6516..7751
                     /gene="preA"
                     /locus_tag="ACHKFK_000524"
     CDS             6516..7751
                     /gene="preA"
                     /locus_tag="ACHKFK_000524"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO9050945.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKALKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANHNIVPAEDLDRSYI
                     VYPHINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IDLGEVKFKKGEKEHPVTL"
     gene            complement(7994..9004)
                     /gene="mglC"
                     /locus_tag="ACHKFK_000525"
     CDS             complement(7994..9004)
                     /gene="mglC"
                     /locus_tag="ACHKFK_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO9050946.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCVIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVVFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(9020..10540)
                     /gene="mglA"
                     /locus_tag="ACHKFK_000526"
     CDS             complement(9020..10540)
                     /gene="mglA"
                     /locus_tag="ACHKFK_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO9050947.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKKINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQSEILRLASLHL"
     gene            complement(10601..11599)
                     /gene="mglB"
                     /locus_tag="ACHKFK_000527"
     CDS             complement(10601..11599)
                     /gene="mglB"
                     /locus_tag="ACHKFK_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO9050948.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11879..12919)
                     /gene="galS"
                     /locus_tag="ACHKFK_000528"
     CDS             complement(11879..12919)
                     /gene="galS"
                     /locus_tag="ACHKFK_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO9050949.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAADNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            complement(13061..14218)
                     /gene="yeiB"
                     /locus_tag="ACHKFK_000529"
     CDS             complement(13061..14218)
                     /gene="yeiB"
                     /locus_tag="ACHKFK_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO9050950.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGGISDSQTSRA
                     WTPDTSAILYEKHWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWRLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLAFVIPVWLANIFFSVIWLRYFRQGPVEWLWRQLTLRAAGPTISKTSR"
     gene            complement(14235..14903)
                     /gene="folE"
                     /locus_tag="ACHKFK_000530"
     CDS             complement(14235..14903)
                     /gene="folE"
                     /locus_tag="ACHKFK_000530"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO9050951.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            15161..15997
                     /gene="yeiG"
                     /locus_tag="ACHKFK_000531"
     CDS             15161..15997
                     /gene="yeiG"
                     /locus_tag="ACHKFK_000531"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9050952.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEQVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWRIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(16029..18020)
                     /gene="cirA"
                     /locus_tag="ACHKFK_000532"
                     /pseudo
     CDS             complement(16029..18020)
                     /gene="cirA"
                     /locus_tag="ACHKFK_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(18312..19781)
                     /gene="lysP"
                     /locus_tag="ACHKFK_000533"
     CDS             complement(18312..19781)
                     /gene="lysP"
                     /locus_tag="ACHKFK_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO9050953.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19986..20867)
                     /gene="yieE"
                     /locus_tag="ACHKFK_000534"
     CDS             complement(19986..20867)
                     /gene="yieE"
                     /locus_tag="ACHKFK_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO9050954.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRICASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20966..22015
                     /gene="yeiH"
                     /locus_tag="ACHKFK_000535"
     CDS             20966..22015
                     /gene="yeiH"
                     /locus_tag="ACHKFK_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO9050955.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            22089..22946
                     /gene="nfo"
                     /locus_tag="ACHKFK_000536"
     CDS             22089..22946
                     /gene="nfo"
                     /locus_tag="ACHKFK_000536"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO9050956.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVAEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIVLDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22950..24038
                     /locus_tag="ACHKFK_000537"
     CDS             22950..24038
                     /locus_tag="ACHKFK_000537"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9050957.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNITPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAIA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(24306..25247)
                     /gene="rihB"
                     /locus_tag="ACHKFK_000538"
     CDS             complement(24306..25247)
                     /gene="rihB"
                     /locus_tag="ACHKFK_000538"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO9050958.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAENT
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMIGLDLTNQTVCTPDVIARIERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            25377..26075
                     /gene="yeiL"
                     /locus_tag="ACHKFK_000539"
     CDS             25377..26075
                     /gene="yeiL"
                     /locus_tag="ACHKFK_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO9050959.1"
                     /translation="MKEIHNNDLKQQLISESAFKDCFSTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYVTLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKFCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFINDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26142..27392)
                     /gene="psuT"
                     /locus_tag="ACHKFK_000540"
     CDS             complement(26142..27392)
                     /gene="psuT"
                     /locus_tag="ACHKFK_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO9050960.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGALDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(27486..28424)
                     /gene="psuG"
                     /locus_tag="ACHKFK_000541"
     CDS             complement(27486..28424)
                     /gene="psuG"
                     /locus_tag="ACHKFK_000541"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO9050961.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQSAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(28412..29353)
                     /gene="psuK"
                     /locus_tag="ACHKFK_000542"
     CDS             complement(28412..29353)
                     /gene="psuK"
                     /locus_tag="ACHKFK_000542"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO9050962.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISVEFLAQHQEFIRGAKVIVADCNLSEEALVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVTSLVENTECLN"
     gene            complement(29809..31500)
                     /gene="fruA"
                     /locus_tag="ACHKFK_000543"
     CDS             complement(29809..31500)
                     /gene="fruA"
                     /locus_tag="ACHKFK_000543"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO9050963.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNEAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(31517..32455)
                     /gene="fruK"
                     /locus_tag="ACHKFK_000544"
     CDS             complement(31517..32455)
                     /gene="fruK"
                     /locus_tag="ACHKFK_000544"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO9050964.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(32455..33585)
                     /gene="fruB"
                     /locus_tag="ACHKFK_000545"
     CDS             complement(32455..33585)
                     /gene="fruB"
                     /locus_tag="ACHKFK_000545"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO9050965.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            33952..35133
                     /gene="setB"
                     /locus_tag="ACHKFK_000546"
     CDS             33952..35133
                     /gene="setB"
                     /locus_tag="ACHKFK_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO9050966.1"
                     /translation="MHNSPAVTSAKSFDLTSMAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35130..35384)
                     /gene="yeiW"
                     /locus_tag="ACHKFK_000547"
     CDS             complement(35130..35384)
                     /gene="yeiW"
                     /locus_tag="ACHKFK_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9050967.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            35539..36111
                     /gene="yeiP"
                     /locus_tag="ACHKFK_000548"
     CDS             35539..36111
                     /gene="yeiP"
                     /locus_tag="ACHKFK_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO9050968.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36334..37800
                     /gene="yeiQ"
                     /locus_tag="ACHKFK_000549"
                     /pseudo
     CDS             36334..37800
                     /gene="yeiQ"
                     /locus_tag="ACHKFK_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            37918..38904
                     /gene="yeiR"
                     /locus_tag="ACHKFK_000550"
     CDS             37918..38904
                     /gene="yeiR"
                     /locus_tag="ACHKFK_000550"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO9050969.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALERWWQQNGGDRQLIHSEHGKIDGHLLDLPRRNLAELPASAAHSHQYSVKKGLA
                     ALSLPEHQRWRRSLNSGQGHQACGWIFDADTVFNTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            38943..39656
                     /gene="lpxT"
                     /locus_tag="ACHKFK_000551"
     CDS             38943..39656
                     /gene="lpxT"
                     /locus_tag="ACHKFK_000551"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO9050970.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPINHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40068..40634
                     /gene="mepS"
                     /locus_tag="ACHKFK_000552"
     CDS             40068..40634
                     /gene="mepS"
                     /locus_tag="ACHKFK_000552"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO9050971.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            40815..42365
                     /gene="pdeN"
                     /locus_tag="ACHKFK_000553"
     CDS             40815..42365
                     /gene="pdeN"
                     /locus_tag="ACHKFK_000553"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9050972.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGLALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTFVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVVYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAEAQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPAHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEREATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTP"
     gene            42453..44267
                     /gene="yejA"
                     /locus_tag="ACHKFK_000554"
     CDS             42453..44267
                     /gene="yejA"
                     /locus_tag="ACHKFK_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO9050973.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGLPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYSKDHKLSDPLATPPLASGPYRITYW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNVTTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKATKLPSARQQGE"
     gene            44268..45362
                     /gene="yejB"
                     /locus_tag="ACHKFK_000555"
     CDS             44268..45362
                     /gene="yejB"
                     /locus_tag="ACHKFK_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO9050974.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45362..46387
                     /gene="yejE"
                     /locus_tag="ACHKFK_000556"
     CDS             45362..46387
                     /gene="yejE"
                     /locus_tag="ACHKFK_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9050975.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLLLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            46389..47978
                     /gene="yejF"
                     /locus_tag="ACHKFK_000557"
     CDS             46389..47978
                     /gene="yejF"
                     /locus_tag="ACHKFK_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO9050976.1"
                     /translation="MTQTLLSIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHHVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSHGSIVFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(47982..48326)
                     /gene="yejG"
                     /locus_tag="ACHKFK_000558"
     CDS             complement(47982..48326)
                     /gene="yejG"
                     /locus_tag="ACHKFK_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO9050977.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(48660..49850)
                     /gene="bcr"
                     /locus_tag="ACHKFK_000559"
     CDS             complement(48660..49850)
                     /gene="bcr"
                     /locus_tag="ACHKFK_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO9050978.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNILFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(49878..50573)
                     /gene="rsuA"
                     /locus_tag="ACHKFK_000560"
     CDS             complement(49878..50573)
                     /gene="rsuA"
                     /locus_tag="ACHKFK_000560"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO9050979.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVI"
     gene            50723..52483
                     /gene="radD"
                     /locus_tag="ACHKFK_000561"
     CDS             50723..52483
                     /gene="radD"
                     /locus_tag="ACHKFK_000561"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO9050980.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVIFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAATRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGSERDVLIDDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            52608..52892
                     /gene="rplY"
                     /locus_tag="ACHKFK_000562"
     CDS             52608..52892
                     /gene="rplY"
                     /locus_tag="ACHKFK_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO9050981.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAVELDH
                     DKVMNMQVKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLLHIDFVRA"
     gene            complement(53031..54038)
                     /gene="yejK"
                     /locus_tag="ACHKFK_000563"
     CDS             complement(53031..54038)
                     /gene="yejK"
                     /locus_tag="ACHKFK_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO9050982.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            54220..54447
                     /gene="yejL"
                     /locus_tag="ACHKFK_000564"
     CDS             54220..54447
                     /gene="yejL"
                     /locus_tag="ACHKFK_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO9050983.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            54467..56227
                     /gene="yejM"
                     /locus_tag="ACHKFK_000565"
     CDS             54467..56227
                     /gene="yejM"
                     /locus_tag="ACHKFK_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO9050984.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            56302..56378
                     /locus_tag="ACHKFK_000566"
     tRNA            56302..56378
                     /locus_tag="ACHKFK_000566"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:56336..56338,aa:Pro,seq:ggg)
     gene            complement(56504..57432)
                     /locus_tag="ACHKFK_000567"
                     /pseudo
     CDS             complement(56504..57432)
                     /locus_tag="ACHKFK_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            57752..58399
                     /gene="narP"
                     /locus_tag="ACHKFK_000568"
     CDS             57752..58399
                     /gene="narP"
                     /locus_tag="ACHKFK_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO9050985.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDSGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(58434..59486)
                     /gene="ccmH"
                     /locus_tag="ACHKFK_000569"
     CDS             complement(58434..59486)
                     /gene="ccmH"
                     /locus_tag="ACHKFK_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO9050986.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(59483..60040)
                     /gene="dsbE"
                     /locus_tag="ACHKFK_000570"
     CDS             complement(59483..60040)
                     /gene="dsbE"
                     /locus_tag="ACHKFK_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO9050987.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(60037..61980)
                     /gene="ccmF"
                     /locus_tag="ACHKFK_000571"
     CDS             complement(60037..61980)
                     /gene="ccmF"
                     /locus_tag="ACHKFK_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO9050988.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVNPQKTAPEAA"
     gene            complement(61977..62456)
                     /gene="ccmE"
                     /locus_tag="ACHKFK_000572"
     CDS             complement(61977..62456)
                     /gene="ccmE"
                     /locus_tag="ACHKFK_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO9050989.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(62453..62662)
                     /gene="ccmD"
                     /locus_tag="ACHKFK_000573"
     CDS             complement(62453..62662)
                     /gene="ccmD"
                     /locus_tag="ACHKFK_000573"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO9050990.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(62659..63396)
                     /gene="ccmC"
                     /locus_tag="ACHKFK_000574"
     CDS             complement(62659..63396)
                     /gene="ccmC"
                     /locus_tag="ACHKFK_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO9050991.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(63438..64100)
                     /gene="ccmB"
                     /locus_tag="ACHKFK_000575"
     CDS             complement(63438..64100)
                     /gene="ccmB"
                     /locus_tag="ACHKFK_000575"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO9050992.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAAVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(64097..64720)
                     /gene="ccmA"
                     /locus_tag="ACHKFK_000576"
     CDS             complement(64097..64720)
                     /gene="ccmA"
                     /locus_tag="ACHKFK_000576"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO9050993.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            complement(64733..65335)
                     /gene="napC"
                     /locus_tag="ACHKFK_000577"
     CDS             complement(64733..65335)
                     /gene="napC"
                     /locus_tag="ACHKFK_000577"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO9050994.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(65345..65794)
                     /gene="napB"
                     /locus_tag="ACHKFK_000578"
     CDS             complement(65345..65794)
                     /gene="napB"
                     /locus_tag="ACHKFK_000578"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO9050995.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(65791..66654)
                     /gene="napH"
                     /locus_tag="ACHKFK_000579"
     CDS             complement(65791..66654)
                     /gene="napH"
                     /locus_tag="ACHKFK_000579"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO9050996.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(66641..67336)
                     /gene="napG"
                     /locus_tag="ACHKFK_000580"
     CDS             complement(66641..67336)
                     /gene="napG"
                     /locus_tag="ACHKFK_000580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO9050997.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDVA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(67343..69829)
                     /gene="napA"
                     /locus_tag="ACHKFK_000581"
     CDS             complement(67343..69829)
                     /gene="napA"
                     /locus_tag="ACHKFK_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO9050998.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEDKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGSNMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(69826..70089)
                     /gene="napD"
                     /locus_tag="ACHKFK_000582"
     CDS             complement(69826..70089)
                     /gene="napD"
                     /locus_tag="ACHKFK_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO9050999.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFTGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(70079..70573)
                     /gene="napF"
                     /locus_tag="ACHKFK_000583"
     CDS             complement(70079..70573)
                     /gene="napF"
                     /locus_tag="ACHKFK_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO9051000.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAANCPVSAITAEY
                     LHAH"
     gene            70682..>70839
                     /locus_tag="ACHKFK_000584"
     CDS             70682..>70839
                     /locus_tag="ACHKFK_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051001.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCFLI"
BASE COUNT        17390 a        18853 c        17396 g        17200 t
ORIGIN      
        1 cggccgcaat cgtaatacca aactcagcaa gtgtggctct taaactattg gcacaggcaa
       61 tccgctcacg gataagtatt ccccgttcag tgtgtaacgc taaaacggcc tgctgctctt
      121 cggtttttat ctgaacaaaa cgcatattcg gacgggttac cgcttcgcag attgcttcag
      181 catctgctgc atccgtcttg ttcgttttca cataaggctt cacatactgc ggaggtatga
      241 gttttacctc atgaccatag cgggtgaata atcgcgcaaa ataatgactg gatgagcagg
      301 cttccatgcc aatcagacaa ggttccagtt gaatcacaaa ctgaacaaac ttagcccggg
      361 tgagtttttt acgcaaaata gtatggcctt catgatcgac accatgaagc tgaaaaacgt
      421 tctttgccag gtcgatacca agagttgata ctttcatgtg gacgctccta atttttcagg
      481 acgtattgca acgatccagt ctggtacttt gataccgtaa aatctggagc gtccatctca
      541 ttatccctgt aaaacgcgcc ctcagcccac atccatgtgg gctgccccag ccttcaggga
      601 acgcctcggc gattttgacg ccaccaaaca accgtgcggc ctattgataa agagctaaca
      661 cattgtcaaa aaacatcact atggtttctt agagtttctc gatatcaatg gcctgaatag
      721 cccttgcaat atcaggggaa ttattcaaca cccgaacatg ctgaaataat tccgttgctt
      781 catcgtattc tttacgcaaa taactcaacc actgtttaat ccgcgcaacg tgatataacc
      841 cggtatcgcc ctgcttttcc agacgggtat atttttgcag caaagcaacc acctccggcc
      901 acggcattcg cggttcgtta tatttcacca cacggctcag gttgggaata ttgagcgccc
      961 cgcgaccaat catcactgcg tcgcagccgc tgatcgccat gcattgttgc gcactctgcc
     1021 agtcccagat ttcaccgttg gcaatcaccg gaatattaag ccgctggcga atctcgccaa
     1081 tcgcctgcca gtcaatatgt tccgcgcggt aaccctgctc ttttgtccgc ccatgtacta
     1141 ccagctccgt agcgccagcc tgttgcaccg catcggcgat ttcaaatttc ttctcaccgc
     1201 tgtcccagcc cagacgcact tttacgctga caggcaaatg cgccggtaca gcttcgcgca
     1261 tcgcttttgc accctggtag atgagttcag gatctttcag taacgttgcc ccaccaccgc
     1321 taccgttaac cgttttcgac gggcagccgc agttaagatc cacgccccag gaacctaact
     1381 ccaccgcacg ggcggcgttc tctgccagcc actgtgggaa ctgccctaat aactgcacgc
     1441 gaaccagcgt accggatggt gtccggctgg cattttgtag ctcagggcaa atgcgatgaa
     1501 agacttttac tggcagcagt tgatccacca cgcggacaaa ctcggtgatg cacagatcgt
     1561 agtcgttaac ttcggtcagt aattcccgca ccaaagagtc aagtacaccc tccatcggag
     1621 ccagtaacac acgcatatca tcacccgcaa aaaaatgagg cgctatgtta gcgcctccgg
     1681 tcaacggatt aaaggtctgc aaagagggat tgctgcaaac tgcaatccct ctctatgctt
     1741 gatgtatgcc tgagttcgct gcgcattgcg taataccggg gagcgaaaaa ataagcgctg
     1801 atttcttaat gtgatcggta gcacgtttta cgaattaatt gtatgataaa tccatctcat
     1861 ctggggtgtt gattatgagt aagacactga acattatctg gcaatattta cgcgctttcg
     1921 tcctgattta tgcctgcctg tatgcaggca ttttcattgc ttccctgcta ccggtaacca
     1981 ttccgggcag catcatcggg atgctgatcc tgtttgtcct gctggccttg caaattcttc
     2041 cggcgaaatg ggtcaatccg gggtgctacg tactgattcg ctatatggcg ctattgtttg
     2101 tgccgattgg cgtaggcgtc atgcaatatt ttgatttgct ccgcgcacag tttggtccgg
     2161 tagtggtttc ctgtgcagtc agtacgctgg tggtttttct ggtggtgagc tggagttcac
     2221 aactggtgca cggtgaacgt aaagtcgtag gtcagaaagg atcagaagaa tgatggcgaa
     2281 tatctggtgg tcattaccgc tgacgttgat tgtctttttt gccgcccgca aactggcggc
     2341 acggtataag tttcccctgc ttaacccgtt gctggtagca atggtggtga tcattccttt
     2401 tttaatgctg actggcatct cttacgacag ctactttaaa ggtagcgaag tgctaaacga
     2461 tctgctgcaa ccggcggtcg tcgcactggc ttatccttta tatgagcagc tacaccagat
     2521 ccgcgcgcgc tggaaatcga tcatcaccat ctgttttatc ggcagcgtgg ttgcgatggt
     2581 gacggggact tccgtggcat tgttgatggg cgcttcaccg gaaatcgccg cgtcaatcct
     2641 gccgaaatca gttaccacgc ctattgcaat ggcggttggc ggcagtattg gcggtattcc
     2701 ggcaatcagc gcagtttgcg tgattttcgt cggcatcctc ggcgctgtgt ttggccatac
     2761 attgcttaat gcgatgcgta ttcgtaccaa agctgcgcgc ggtctggcaa tgggcacagc
     2821 ctcgcacgcc ctcggtacgg cgcgctgcgc cgagctggat tatcaggaag gtgcatttag
     2881 ttcgctggcg ctggtgttat gcgggattat tacttcgctg atcgcaccgt tccttttccc
     2941 gattattctg gcagtaatgg gctaaaattt gcgatgcgtc gcgcattttt gatgtaagtt
     3001 tcacgcgttg cataattaat gagattcaga tcacatataa agccataacg ggttcgtaaa
     3061 ctgttatccc attacatgat tatgaggcaa cgccatgcat ccacgttttc aaaccgcttt
     3121 tgcccaactt gcggataact tgcaatctgc actggaacct attctggcag acaagtactt
     3181 ccccgctttg ttgaccgggg agcaagtctc atcgctgaag agcgcaacgg ggctggacga
     3241 agacgcgctg gcattcgcac tacttccgct ggcggcagcc tgtgcgcgta cgccattgtc
     3301 gaattttaat gttggcgcaa ttgcgcgcgg tgtgagcgga acctggtatt tcggtgccaa
     3361 tatggaattt attggtgcga caatgcagca aaccgttcat gccgaacaaa gcgcgatcag
     3421 ccacgcctgg ctgagtggtg aaaaagcgct tgcagccatc accgttaact acacgccttg
     3481 tggtcactgc cgtcagttta tgaatgaact gaacagcggt ctggatctgc gtattcatct
     3541 tccgggacgc gaggcacacg cgctgcgtga ctatctaccg gatgcctttg ggccgaaaga
     3601 tctggagatt aaaacgctgc tgatggacga gcaggatcac ggctatgcgc tgactggtga
     3661 tgcgctttct caggcggcga ttgcggcggc aaaccgttcg cacatgcctt acagtaagtc
     3721 gccaagcggt gtcgcgctgg aatgtaaaga cggtcgtatt ttcagcggca gctacgctga
     3781 aaacgccgca ttcaacccga ctctgccgcc gttgcagggg gcgttaattc tgttgaatct
     3841 caagggttat gattacccgg atatccagcg cgcggttctg gcagaaaaag tcgatgcgcc
     3901 gttgattcag tgggatgcca cctccgcaac gctgaaagct ctcggctgtc acaatatcga
     3961 ccgcgtgctt ctcgcttaag cctggtgccg gatgcggcgt gaacgccttg tccggcttgc
     4021 cagccctttc ctggtgtcga aaatcccggc aaacagtttg ccgtttcttg cgcaaaacca
     4081 gcgggtaaag tagcctgatg gaaattttcc ttagatcgag tctcctgcat gttaaagcgc
     4141 gtgttcctca gcctgttagt cctgatcggc ttgctgctgt tgactgtgct cggcctcgat
     4201 cgctggatga gctggaaaac cgcgccttat atctacgacg aattgcagga tctcccctac
     4261 cgccaggtcg gtgtggtgct cggcacagca aaatattatc ggactggcgt aattaatcag
     4321 tattatcgct accgcattca aggagcgatt aatgcctata acagcggtaa ggtaaattat
     4381 ctattactga gcggcgataa cgcattgcaa agttataatg agccgatgac catgcgcaaa
     4441 gatttaatcg cagctggtgt cgacccatca gatattgttc tcgattacgc aggctttcgt
     4501 acgctggatt ccatcgtgcg tacacgcaaa gttttcgata ctaatgattt cattattatc
     4561 acccaacgtt tccactgtga gcgggcatta tttattgcgc tgcatatggg gattcaggct
     4621 cagtgttatg ccgtaccgtc accgaaagat atgctgtcag tacgtattcg cgaatttgcc
     4681 gcccgtttcg gtgcgctggc tgacctttat atttttaaac gtgaaccgcg ttttttaggg
     4741 ccgctggtcc ctattccggc tatgcaccag gtgccggaag atgcgcaggg gtatcccgcc
     4801 gtcacacccg aacagttact tgaattacaa aagaaacaag gaaagtagtt atggatgtac
     4861 agcagttttt tgtcgttgcc gtttttttcc ttatcccgat attttgtttc cgcgaagcat
     4921 ggaaaggctg gcgcgcaggc gcgattgata aacgggttaa aaatgcacca gaaccggtgt
     4981 atgtctggcg agcgaaaaat cccggactct tcttcgctta tatggtggca tatatcggct
     5041 tcggaatttt atctatcggc atgattgttt atcttatttt ctatcgttaa tattttccct
     5101 tttctgttaa ttattgagaa taattattac ttcactcaat aagctgcgga tataacattc
     5161 tgaaccgcag ctatttgtga atcctttcac agtttaaata tccccacgcg catagcctta
     5221 atatcagcac gcaatattta tttagcgttc gccttaatta ctcatagcat taaggaagat
     5281 cacatgccgc aacaaaatta tctggatgaa ctcactccgg cttttacgcc tttactggcg
     5341 attaaagaag cctctcgctg tttattatgt catgacgctc cctgtagtca ggcttgcccg
     5401 gcacagaccg atccggggaa atttattcgc tcaatctact ttcgtaattt taaaggcgct
     5461 gccgagacaa ttcgcgaaaa taatgccctc ggtgccgttt gtgccagagt gtgcccgacg
     5521 gagaaattat gccaaagcgg ttgtacacgt gccggtgttg atgctcccat tgatatcggt
     5581 cgcttacagc gttttgttac tgattttgaa caacaaactg ggatggaaat ttatctgccc
     5641 ggtactaaaa cgctcggcaa agtcgccatt attggcgcag gtcctgccgg attgcaggcc
     5701 agtgtgacgc tgacaaacca gggttatgac gtcacgatct atgagaaaga agcacaaccc
     5761 ggtggctggt tgcgtaacgg cattccgcaa ttccggttac cgcaatcggt gctggatgca
     5821 gaaatcgccc gtatagaaaa aatgggcgtg accattaagt gcaataacga aatcggtaaa
     5881 acactcaccc tggagcagct gaaagcggaa aaccgcgcgg tactggtcac cgtagggtta
     5941 tcgagcggtt ccgggttatc gctgtttgag catagcgacg ttgagattgc cgtcgacttc
     6001 ctgcaacgtg cacgacaggc gcaaggcgat atcagcattc cacaaagcgc attaattatc
     6061 ggcggcggtg atgttgcgat ggacgtagcc agcacgctga aagttctcgg ctgtcaggcg
     6121 gtaacctgcg tagcgcgtga agagttagat gagtttccgg caagcgaaaa agagtttacc
     6181 agcgcccggg agctgggcgt ttcgatcatc gatggattta caccagtagc cgtggaaggc
     6241 aataaagtca cctttaagca tgttcgttta ccgggcgaac tgacgatggc ggcagataaa
     6301 attattctcg ccgtcggtca gcatgccaga ctggatgcct ttgcggagtt agagccgcag
     6361 cgtaacacca tcgaaacgca acattaccag acccgcgacc cgcaagtctt tgctgctggc
     6421 gatattgttg agggtgacaa aaccgtggtc tatgcagtga aaaccgggaa agaagccgcc
     6481 ggggcgattc atcactattt agagggagct tgctcatgtt aacgaaagat ctttcgatta
     6541 ctttttgcgg cgtgaagttt cccaacccgt tctgcctttc ttcttcgccg gtaggcaact
     6601 gctatgagat gtgtgccaaa gcctacgaca caggttgggg cggcgtggtg tttaaaacga
     6661 tcggtttttt tatcgccaac gaagtctcgc cgcgttttga tcatctggtg aaagaagata
     6721 ccggttttat cggcttcaaa aatatggagc agattgctga acatccgttg gaagagaatc
     6781 tggccgccct gcgtcggctg aaagaagatt atccggacaa agtattgatc gcttcaatca
     6841 tgggcgaaaa tgagcagcaa tgggaagagc tggcgcgtct ggtgcaagaa gctggcgcgg
     6901 atatgatcga gtgtaacttc tcctgtccgc aaatgacttc tcatgcgatg ggtagcgatg
     6961 tcgggcaaag cccggagctg gtagaaaaat attgtcgggc agtaaaacgt ggttccacgc
     7021 tgccaatgct ggcgaagatg acgccgaata ttggtgatat gtgcgaagtg gcgctggcgg
     7081 cgaagcgcgg cggcgcagat ggcattgcgg cgattaacac cgttaaatcc atcaccaata
     7141 tcgatcttaa tcagaaaatt ggtatgccga tcgttaacgg taaatcgagt atttccggat
     7201 attccggtaa agcgctaaaa ccgatcgccc tacgctttat tcagcaaatg cgcacccatc
     7261 cagaactgcg cgatttcccg atcagcggta tcggcggcat tgaaacctgg gaagatgcgg
     7321 cggagttttt attgctcggt gcagcgacgt tgcaggtgac caccggcatt atgcagtacg
     7381 ggtatcggat agtggaagat atggcaagcg ggttgtcgca ttatctcgcc gatcaagggt
     7441 ttgattcgtt gcaggagatg gtaggtctgg cgaatcacaa tattgtcccg gcggaagatt
     7501 tagaccgcag ttatattgtc tatccccata tcaatcttga taaatgtgtt ggctgtggac
     7561 gctgttatat ttcctgttac gacggcggtc accaggcgat ggaatggagc gagaaaaccc
     7621 gcacaccgca ttgtaatacc gagaaatgcg tgggttgtct gctttgtggt catgtctgcc
     7681 cggtaggttg tattgatctt ggggaagtga agtttaagaa aggcgagaaa gaacacccgg
     7741 taacgttgta aaaccagcga cgcatccggc atttagttcc tgtcgccgga tgcggcgtga
     7801 acgccttatc cggcctacaa aaccataaaa atcagtatgt tgagcttatt ataggccgga
     7861 taaaacgcgc cagcgttgca tccggcattt ggctcctgtc gccggatgcg gcgtgaacgc
     7921 cttatccgga ctacaaaacc acacagatca tgatgttatg tgcgtttatg aacgaatgcg
     7981 gaccacaatc acatcacttc ttacgcgcgt atttcagtga atccagcgcc acggcgaaga
     8041 taataatcgc ccctttgatg atgtactgcc agtacgggtt tacgccgata tacgtcaggc
     8101 catagttaat gacggtaaaa ataattaccc cggtcaccac accaattacc gtccccacac
     8161 cgccgctgaa cgatacgccg cctaccacgc acgccgcgat agcatccagc tcatacataa
     8221 agccgaggtt gttggtggct gagccgatac gtccggcttc taacatcccg ccgaaggcat
     8281 agaacacacc agacaacgcg taaatcatca gcaggttcag gccgacgttg acaccagata
     8341 ctttcgccgc ttccgggtta ccgccaatgg caaaaatgtt cttgccgaag cgggttttgt
     8401 tccataacac ccagacaaac accaccgcaa tcaacgcgta gaaggtgatg taagagagac
     8461 ggaaactccc cagcgcgaca aagccttgag caaaggtaga gaagccactg tcaaaaccgg
     8521 aaattggcga cgccccgaca aagtcgtaat agagcgagtt aatgccgtag acgataatca
     8581 tcgtaccgag cgtggtaatg aacggcgtca cgttgagata agcgataatc aagccgttga
     8641 tcaaaccgat caccgcacca atgacacaga caatcagaat aaccagcgca atcggcatcg
     8701 tcgccatttc cgggaacact ttgttggcgt tatccatgga ctgcaataac gtcgctgcta
     8761 ctactgccgc cagccccacc tggcgacctg cggaaaggtc agtcccctgg gtgacaatta
     8821 accctgccac accaagcgcg ataataatac gcaccgatga ctgggtgaga atattactta
     8881 agttcaacag acttaaaaat gttgggtcct ggaaaataat aatcgccagc aaaactaaaa
     8941 gaacgacgta aataccgccc tctttcaggt aagtaagaaa acttttctta tttaacgcac
     9001 tcatgggaag cccctaatct taaaggtgca aagacgcaag acgcagaatt tcgctttgcg
     9061 ttgttgtttt tgtatcgaca attccggaaa cgagaccatt gctcatgacc agaatacggt
     9121 ctgttatccc taacaactct ggcatttcgg aggagataat aataatcccc ttgcctttct
     9181 tcgccagttc ggcaattaac tgataaatct caaacttagc cccaacatca ataccgcgcg
     9241 tcggttcatc gagcattaat atttctggtt gcgttaacag ccagcgacca ataatcactt
     9301 tttgctgatt accaccggag agcgaaccaa tttgcgtccg atgccccggt gtttttaccc
     9361 gcatcgaatc aatcacccac tgggtatcgc ttttcatccg cgagttatcc agtaaaccaa
     9421 ctttattttt atagttgcgg atattggaaa ttaacgagtt aaagccaata tccagatagg
     9481 cataaattcc cgttgagcgg cgctcttcag ttaccagtgc aaatccgtgg tttatggctt
     9541 cgttggcatt atggttattg atctttttgc cgtgcaacgt aatggtgcca gccgatttct
     9601 cgcgaatacc aaataacgtc tcaacaatat cggtacgttt cgctcccacc agaccggcta
     9661 taccgagaat ctccccttta tgcagatcaa acgagacatc gcgtatcgac ggctggcgca
     9721 gcgacgtcag gttacgcact tccagaatca cttcgcccgg cttgttttct ttgtcaggga
     9781 aacgctggtt aagagaacgc ccaaccatca tggcgatgat cttgtccatc gtcagtcctg
     9841 ccagcggttc ggtagcgatc cactgaccgt cgcgcaatac ggtaacttca tcacataact
     9901 ggaagatttc ttccatttta tgcgagatat aaacaatacc gcagccgcgc tcttttaatt
     9961 tacgaataat agtgaacaga tgattgacct ctttttcggt taacgaagaa gtcggttcat
    10021 ccataatcac aattttcgcg ttataggaaa acgctttggc aatttcgatc atctgcattt
    10081 gcgaaacaga taatgtaccg acgcgcgcac gcggatcgat atcaatatcc agttcatcaa
    10141 aaatcgcttt ggtttcgcgg tacattttgt cctgatcgac aaacatgcct ttggtgggat
    10201 atcgccccag ccacatgttg tccatcaccg aacgttgtaa taccaggttt aactcctggt
    10261 gtaccatcga aataccattt tccagtgctt ctttggcgga atggaaatcg atctctttac
    10321 cctggaataa aatggtgccg gagtcttttt gataaatacc aaacaggcat tttaataatg
    10381 tcgattttcc tgcaccgttt tcccccatta atgcatggat agaatgtggt cggactttta
    10441 aattaacgtt atcaagtgcc ttaacaccag gaaaagactt gttgataccg ctcatttcca
    10501 acaagtattc cccggaagac ggagtcgttg agctgaccat ataattttac cttgttggcc
    10561 atacaataag ggcgcagtaa acgactgcgc ccaatcagtc ttatttcttg ctgaattcag
    10621 ccaggttatc tttatctacg ccaacataag gtacgcggac cactttgttg tcgattttcc
    10681 agttggtgcc atcagccgca cctttgccat cggccaggtt tttcgccaga tcaaaggtcg
    10741 ctttcgcctg gttgttagca tcgttcagta cggtgcccgc cagtgcaccg gatttcacca
    10801 gcgccagcgc ttctggcagc gcatcgacgc caaacaccgg aatgctggac ttgttgtgtg
    10861 ctttcagtgc ttctaccgcg cccattgcca tcgcgtcgtt gttggcgata accacctcga
    10921 ttttgttggc gttcgggcca gacagccagg cgtccatctt atctttcgcc tgagcggtat
    10981 cccacatagc ggtatctaac tgtaactgtt cagttttaat gcctttgtcg ttcaattctt
    11041 taatcacgta agtggtacgt gcttctgcat ccggatgccc cggctcacct ttcagcagta
    11101 cgaattgaat ctgaccgtct ttgttcagat cccaaccctg attcgccgcc cagtgtttag
    11161 caatcaaatc gccctgaata atgccggact ctttggagtc agtgccaacg tagtaggctt
    11221 tgtcgtagct atccagcgcc ttacgagacg gttctttgtt gaagaaaacc accggcacgt
    11281 tttgcccacg cgctttctca atcaccgtac ccgcagctgc cgggtcaacc aggttgattg
    11341 ccagtgcctt cacccctttc gccagcaata cgtcgatctg atcgttctgc ttggactggt
    11401 cattctgaga atcattcatc agcagctgaa catctggcgc ggctttcgca tcttgctcaa
    11461 tagccttgcg cactacagac ataaagttat cgtcgtactt atagattgtt acaccaatgc
    11521 gagtatcagc agcgtgtgca gcggcaccga ataacatgct ggccatcaca gcagacaggg
    11581 ttaacacctt cttattcatg gtatctccgg tttttcttat gcagggtagt gcttgagata
    11641 aatgctcggc ggggcagtag agttaatgaa gtgttactga acgccgaagc tcacttttta
    11701 aaattcgttc ttccatgccc ggtaacgctc cagaaaacgg ctttaattgt tgttatgacg
    11761 ctattacctc ggtgaaagtg attattcact gttacatacg ggttacaacg ttaaaacggt
    11821 gcaatcatag ccatcacatt gttaagatac tgtgaaatca ctcacagatt gaaagcggtt
    11881 acatcgcctg atttgttgag ttagtgatcg ccgccgcatt ctggcgtgtt gcgacagaat
    11941 ggcgacgcac taacgtcggc ataaaacagt ggctggcacg aggatcaata ttgtctgctg
    12001 ccccctgcaa ggccagttcg gtagctaatt tagccattga agcaatggga taacgcacag
    12061 tcgttaattg cgggtcggtg taacgggcga tgggaatatc atcgaaacca atgattgaga
    12121 gatgtaacgg aatcgcaatg ccattatctt ttaatgctgt cagtgcgcca gcggccatat
    12181 tgtcgttata agcaaataca gcggttagtt gtagattgcg ccccagcagt tcaaccatcg
    12241 ccgcctcacc gcccggcatg tccggcgtgc cagtgccaat ccagctttcc ggcggaataa
    12301 tatcctgctc tttcaacgca ctcatccagc ctgctttacg catggcgtca tcttcaatgc
    12361 cgtggctgga agaaagataa ccaatacgtt gatgaccgtt attcagcaac atgcgcgtcg
    12421 ccattcgggc accgctgaga ttatccaggc aaacgcaacg atgggcgtac cccggcacaa
    12481 cgcggttgat taacaccata ccgggaatgt tatccataaa ttgtgccagt tcatcgtcac
    12541 tcaatgcttt tgagtgaaca atcaacgcat tacaacgctg gcgaattaac acctcaatgg
    12601 cgtgacgctc tttttccgct tcatgatagc tattgccgat tagcacgtat ttctgatgct
    12661 gctgggcgac cagatccacc gcttttacca gcgcgccgaa aaacgcatca gaaacgtcca
    12721 tcaccaccac gccaatggta tcgctaacct gagttgccag cgcctgggca ttggcgtttg
    12781 gccgataatc cagctcactc acagctttca ttactgcttc acgcgtgtcg gcactgacca
    12841 gcgtgctgtt attgagcacc cgggaaaccg ttgccacaga gacgccagcc tgacgcgcta
    12901 catcacgaat ggtgatcata ttcactacct gttatgagat tgcagtaaat gactgcttgc
    12961 tggcggctat tttgtcagca ctgaatacag gacttcgtga atcgagtcac agcaatggaa
    13021 acggttacag ccgttttgtt aacgaatgtg atccagatcg ttatcttgat gttttagata
    13081 ttgtcggtcc ggcagcacgc aaagttaact gacgccagag ccattccacc ggcccctggc
    13141 ggaagtaacg cagccagata acagagaaga agatattcgc caaccatacc ggaataacaa
    13201 acgccagcag ttccagacgg tcaaactgca taaacaaacc gaggtggtaa aaaagcgtag
    13261 tacaaatcag cgtttgcaat agatagttgg tcagtgccat ccgtccgaca caggcgattg
    13321 ccagcaccag cttaaagcgg cttaattgcg gccagaagcc ataaaacagc gacgcatagc
    13381 caatcgcctg aaacggcgca ctcagttccc gcggcatctg aagtaagaag gcgcaccaac
    13441 gatacgccca gtccagtcgc cattgcaggg cgatggcagg aaggttaatg atcaccccaa
    13501 tcgccaccag cacaaaacca gtacgacgat agtgacgtaa gctgaactgc cctttcagcc
    13561 agccactgcg catcaatgcg gcaccaatga gcatcatccc tgccagttgc cagccatact
    13621 gtgcgcccag tgccagtaaa ctgttgccaa caccatcggc acgattactg atcgcttcaa
    13681 caccaccgtg aagtttccag tgcttttcat acaaaatagc cgatgtatca ggtgtccagg
    13741 cgcggctggt ctggctatcg gaaatccccc ccaataacag caaaacgcca agccccacca
    13801 gataaagcat gacgccggta ttaaacaggc tttttaccga tggcgcatcg cgcaccagcc
    13861 gccagcagat taagcccacc agcccgtaag ccagcagaat atcgccgtcc cagaacaata
    13921 aaccgtgaat aaagcccagc aagactaaca gcgttaaccg cgactggatc catcgtctgc
    13981 cacggggcag caacatttgc aggcccgcac caaacaacag cgcaaaaagc gtgaggaatt
    14041 tcacctggcc gatgagatcg agaaatgccc aggtccatgc atcctgtggc gtaatagcgc
    14101 cgtaccaggc gggattgaga taagccgcct ttggtaaccc aaaggcgctg atgtttaata
    14161 gcaggatccc cagaatggcg acgccgcgaa caaaatcgag cgtgacgttg cgctccacgg
    14221 ttcctgcctt tttatcagtt gtgatgacgc acagcgcgca gaaactcgtg gcgcgtattc
    14281 tgactggatt tgaacaatcc accaagagag gtcgttgtcg tggcactggt tgcatcgcgg
    14341 atgccacgcg ccttcacgca gtaatgcacc gcgtcgatag agacagccac gttattggtg
    14401 cccagcagcg tttgtagcgc aatgagaatt tgctgcgtca gacgttcttg tacctgcgga
    14461 cgctgggcaa agaactgcac aatacggtta atttttgaca gaccgatgac cgaatctttc
    14521 gggatatagg ccaccgtcgc tttgccatcg atggtaacaa aatggtgttc acaggtgctg
    14581 gtcagagtga tatcgcgcac ggtgaccatt tcatcgacct tcattttgtt ttcaatgagg
    14641 gtgattttcg ggaaattggc gtaatccaga ccggagaaaa tttcatcgac atacatttta
    14701 gcgatgcgat gcggcgtttc catcaaactg tcatcagcca ggtcgagatt cagcagctgc
    14761 atgatttcgg tcatatgacc agcaataagg cttttgcgcg tttcgttatc catttcatgc
    14821 acgggcgggc gcagcggtgt ttccagtcct cgcgcaacta acgcttcatg aaccagggcc
    14881 gcttctttac tgagtgatgg catttatgat ttctcctgca ggtgtgacgc ctccgccctg
    14941 cgtgggggca aagttattaa gctgatttac agcctgatta ttgtgcgtga ggcggcgcac
    15001 ataatccagt attcacagcg ataattattg taattgccgc tgcctttcat cagcagatgt
    15061 taaaacatcg ttatgcaaat acggaagtga aagttactca cagcacattg aataaacggt
    15121 atgatgaaga aattgcaaac aacacaacaa ggagccacgc atggaaatgc tcgaagagca
    15181 ccgctgtttt gaaggctggc agcaacgctg gcgacacgac tccagtacct taaactgccc
    15241 gatgacgttc agtatctttc tcccaccacc tcgtgatcac accccgccac cagtgctgta
    15301 ctggctttcc ggattaacct gcaatgacga gaacttcacc accaaggcgg gtgcccagcg
    15361 cgtggcggcg gaactgggga ttgtactggt gatgccagac accagcccgc gcggcgaaca
    15421 ggttgccaac gacgatggct acgatttagg ccagggcgcg gggttttatc ttaacgccac
    15481 gcaaccaccg tgggcgacgc attaccggat gtatgattat ctgcgcgatg aattaccggc
    15541 gctggttcag tcgcaattta atgtcagcga ccgctgcgcc attagcggtc attccatggg
    15601 tggtcacggt gcgctgatta tggcgctgaa aaatccgggt aaatacacca gcgtttccgc
    15661 ctttgcgcca attgtgaatc cgtgcagcgt cccgtggaga atcaaagcgt ttagcactta
    15721 tttaggtgag gacaaaaatg catggctgga atgggacagt tgcgcgttga tgtatgccag
    15781 taacgcgcag gatgcaatcc cgacgcttat tgatcagggt gataatgatc agtttcttgc
    15841 tgaccaactg caacctgcag tactggcaga agccgcgcgc cagaaagcgt ggccgatgac
    15901 gctgcgtatt cagccgggat atgatcacag ttactacttc atcgcctctt ttatagagga
    15961 tcacctgcgc ttccatgcgc agtatttact gaagtgaaag tccgcccggt tcgccgggca
    16021 tcttctcatc agaagcgata atccactgcc ataaagtaac gacgtccgtc ttcgttatag
    16081 ctgtagtcgt cacgactgag atccttgtcg ccaaggttca gcacgcctgc gcgcagtttg
    16141 acgtctttag tcacctgcca ggccgcgccg gtattccaga tggtataacc gcccggtgtt
    16201 ttagccgtcg cgctgtcggc gcgtttctgc ccggtatagt gaccagaaac atagaatgac
    16261 cagtcttcca gcgccagcgg tttccagtcc agcgtaccgt tagcagtatg gaacggcaga
    16321 tcggatagcg gtttgttttc gccgttgctg acatcacgac catcgttgta ggtgtagtta
    16381 atcgacagtt tccattcatc gttgaacgga attttcagtt cggtttccac gccctgaata
    16441 cgagctttgt taacgttgta gtagctaaat accggtatgc gccgtccgtt agcgcccgtc
    16501 tcaaaaccga caaagtttta gtagcccggt gcagcattga cgtcagacgt acggctaatg
    16561 ctgatacgat ctttcacatc gttacggaaa acggtaacgc tggattcaac cccttccagc
    16621 cagccttctt cacccatgta gtaaagcccc agctcccaac tttcgctggt ttctggtttc
    16681 agatccgggc tacccacaat cttacatgcg ccacggcagg aattgctcgt ccagtcaggg
    16741 ctaagttgca acagagaagg ggctttaaat gccgtcgccc agcccccttt caccgttacg
    16801 gtgtcggtgg cgttataaac caggtaggca cgcggactcc agtgttcacc gtaggtttcg
    16861 tgatcgtcca tacgcacgcc ggtcgtcagc gccagcggct cgaagatccg ccattcatct
    16921 tccacaaaca gcgcgtactg gctggcagac gttttggagc tggttccccc ggtcaggttc
    16981 accgcatcgc taagtttgtc gtgacgccat tcaccgccaa ccgtgagaaa ctgattaatc
    17041 gccgtcagcg gcaacgtgta tttgccgtcg accgtattgc tttcggaagt tatcgggctg
    17101 ctgttgccag ggtttttgtt ctcgactttc tcaccgtagt atttcagttc gctggtgccg
    17161 taatcccaac gcccattatg gctgacggag tagttctggc gttccaggcg gtttttgtcc
    17221 agcgagtcgg aatcacgatc ctgacggtcg aaaccgtatc cggcagtaaa atcgtgattt
    17281 tgattcggtg tccaggcaaa ttcgacattg ccgtcgcggc tggagaatcc ttcaatacgc
    17341 ggcgtttctc cggtatcggt ggtcgttgag ttttgcgggt cattcttttc acgttttgcc
    17401 aggctgccgt aagctttcat tcccagcacg ccgtcaatta atggtccgct ggtaaagaat
    17461 tgaccgttat aggtatcacc gcgatcgcga tgttcctgaa cggtggtatc gacggtaacg
    17521 gtgcccgacc atttctgacc gatttttttg gtgatgatat tcactacacc gccgagcgca
    17581 tcggaaccgt acagcgacga catcgggcca cgaaccactt caatacgttc gatggaatcg
    17641 accgggatcc agttcagatc gaaatcattg tggcggaaga cggcattgcg ggagttaacg
    17701 cgtttaccgt cgacaagaat cagggtgtag ctgctgtcca gaccacgaat acttacgccc
    17761 ttacggttat ccccttcgtt cgtcagttgt acgccaggca cttctttgag gacatccttc
    17821 agattctgta ccggttttcg ctgcaggtct tcctgggtaa tgacgctgat actggcggga
    17881 gcatctttaa ggttttgttc aacggaagat gcagtgacaa ccatcgtttc gccatcatca
    17941 tcgaccgcta acacaggcca tgcacaagaa atagcggaca aacacagccc gacccgtacg
    18001 aaagggttca acctaaacat tccatatctc catgaggtaa ctacgaaaat aaaatgggta
    18061 tcgctcacat cttctttacg tccccttgcg tacggcagca tcgcggtgga cttatttgta
    18121 tgcaggtgat catcccagaa agccggaaca gcctctgatt tgtcagcctt ttttgattgc
    18181 ggcgtaacga taatgcaaac gataacaatt atcaatccaa atgttaaatt ttatatccgc
    18241 atgatttgtc agggaaatgg aattgaaaaa gccctctcgg ttgagagggc ttagcaagga
    18301 agggaggaaa cttatttctt atcgttctgc gggaacttca tttcgctgta gcgtacgaag
    18361 tgagttcctt taatcagctt gtagccgaac caaataatca ggaacagcgg gataccaata
    18421 tacgttgccg ctacgccgcc ccagtcaatg gtatctttca ggaacgcttc gtagttctgg
    18481 cccaaagtga taatcagaca cagaatgaat gcgaagatcg gccccagtgg gaagaaacct
    18541 gaacggtacg gcagatcgtt aatgtcgtgt ccctgcaata cgtaaccgcg acggaagcga
    18601 tagtggctaa tggcaatccc cagccaggcg ataaaacccg tcatcccgga ggtgttcagc
    18661 agccacaggt ataccgtctg gttgccaaac atggaggtca ggaagcacaa accggcaatc
    18721 accgtcgtcg catacagcgc attacgcggc acgccaccac gcgacagttt agcgaaaatg
    18781 cgcggcgctt taccgtcaca cgccagggta tacagcatac gagtagacgc atacataccg
    18841 gagttacctg ctgacagcac cgccgtcaga ataactgcgt tcatcaccgc cgccgcagag
    18901 agcagacccg cgtgctggaa caccagggtg aacggactga cgctgatgtc tttaacatcg
    18961 ttacgcagca ggctcggatc ggtatacgga ataatcaggc tgataatcag gatcgcgaac
    19021 acatagaaca acaggattcg ccagaatacc tgacgtaccg cgcgtggaat gtttttcgcc
    19081 ggatcttcgg actcgcctgc agcaataccg atcagctcgg ttccctggaa agagaagccg
    19141 acaatcatag ctacgccgat catcgccgca aaaccaccag caaacggcgc ttcgccgatt
    19201 gtccagttgc tccagcctgc aggctgcgcg cctttgaaga taccgataat catcagcacg
    19261 ccaacgatga taaagacaat aactgtcgtg actttgatca gtgagaacca gtattccgct
    19321 tcaccaaagc cgcgaacaga gatgtagttc aacaggaaga taacgccgag gaacaacgca
    19381 ctccagatcc agcccggtgt atccgggaac caccagctca tgaccagctg tgctgcaacc
    19441 aggtcaacgg caatagtcac cgcccagttg taccagtagt tccagcccag cgcgaagcca
    19501 aagccttctt caacatagtt ctgaccgtaa gtggcaaacg aaccggaaac cggcatatat
    19561 gcagccagtt caccgagact ggtcatcagg aagtaaacca tcaggccaat cagcatatac
    19621 gagagcaatg ccccgcccgg acctgcctga gaaatcgttg cgccagaggc aacaaaaaga
    19681 cctgtaccga tggaaccgcc aatggcaatc atcgtcaggt gacgcgcctt caattcacgg
    19741 cgtaagcccg gcgcttctgt agttttagtt tcggaaccca tacaaaaatg ctatccatct
    19801 taaaaatgag gcgcgattgt agcagacgat tggcaatcct tccggcagaa atacacggtt
    19861 ataagacacc ttcatgatcg cccagggatt ataagtaaag cagccaatct cttttctggc
    19921 gaatgcatgc tgagggcgct gcgtcacgaa atcgacacag caccagcatg ttcttgtaca
    19981 gcaccttaac gcggtacatt cgcgggatcg caatagtcca gaaagcgccg tagcgcgttg
    20041 gaaaggtgtt tttgccgatg atgtatacgc cacaacgtac gcatcaggcg cggcagaggg
    20101 accgcaactt cacttaatgt gcctgcctgc aattgatctt caatcacccg tcgcgacagg
    20161 caactaattc ccaacccatg acgcaccgca tgtttgattg cctcggagtt acctaattcc
    20221 atcgccatct caaacttcgg taaatgtgac agcaacagat aatcgacaat ctcccgcgtg
    20281 ccggaaccgc gttcacgcag gatccacggc gcagcggcca gctgttctaa ggtgaccgga
    20341 ccacgagcca acggcgaagt cggcgcggcg aaaaccacca gctcgtcttc cagccacggt
    20401 tcagaaatga tttcagtgct gtggcacggt ccttcaataa agccaatatc aacgcggaaa
    20461 tccagcaccg cttgcatcac gtcctggcta ttcccaacgc taagttcaat cggcaactgc
    20521 ggataatcat ggcgataacg ggcgataact gcaggcagaa tgtagttacc gatggtacta
    20581 ctggcacaga tacgaatcgc gccgttgtct tcgcgaaaca gttgttcgat ttcaaccgcc
    20641 tgttcaagca gtgccaacgc tcgcggatag agcagccgcc cgtgttcatt aacaaccagt
    20701 cttttcccca cgcgatcaaa cagttgaacg ccaagctgcc cttccaggtc ggtcaaggct
    20761 gcgctcactg ctgattgcga caacgccagc atcaccgacg cctgggtggt tgatccactt
    20821 ttcaatactt ctgcaaaaac ttccaactgc cggagggtga tgtgcatagt cgcttaccac
    20881 ttataaagat taattataaa tatataatca attttatttt taaaccagtt agtcgtaacc
    20941 ttatgcccgg ttaaaggaga gggttatgac gaatatcacc ttacagaaac aacatcgtac
    21001 actgtggcat tttattccgg ggttagccct gagtgcagtt atcaccgggg tcgccctgtg
    21061 gggtggttcc attcccgcgg ttgcgggtgc cgggtttagt gccctcaccc tcgcaatctt
    21121 gttggggatg gttttaggca acaccattta cccgcacatc tggaaaagct gtgacggtgg
    21181 tgtgctgttt gccaagcaat atttattacg tctgggtatc attctttatg gcttccgtct
    21241 gacgttctcg caaattgccg atgtcggtat cagtgggatc atcattgacg tgttgacgct
    21301 gtccagtacc ttcctgctgg cttgcttcct ggggcagaaa gtgtttggtc tggataagca
    21361 caccagctgg ttgatcggtg ccggtagcag tatctgtggt gctgccgcgg tactggcgac
    21421 tgagccggta gtgaaagcag aagccagtaa agtaaccgtg gctgttgcaa ccgttgttat
    21481 cttcgggacc gtcgcgattt tcctctaccc ggcgatatat ccgctgatgt ctcaatggtt
    21541 tagtccggaa actttcggta tctacattgg ttctactgtg cacgaagtgg cgcaggtggt
    21601 ggcggcaggt catgccatca gcccggatgc agaaaacgca gcagttattt ccaaaatgct
    21661 gcgcgtgatg atgctggctc cgttcctcat cctgctggcg gcgcgtgtta aacagctgtc
    21721 tggggcgaac agcggcgaga aaagcaaaat cactattccg tggtttgcca tcttgttcat
    21781 cgtcgttgcc atctttaact cgttccacct gttaccgcag agcgtggtga acatgctggt
    21841 aacgctggat accttcctgc tggcaatggc gatggcggcg ctgggtctga ccactcacgt
    21901 cagcgcgctg aaaaaagccg gggcgaaacc tctgctgatg gcactggtac tgtttgcctg
    21961 gctgattgtt ggtggtggtg cgattaacta tgtgattcaa agcgtcatcg cataaaccac
    22021 tacatcttgc tcctgttaac ccgctatcat taccgttttc ctccagcggg tttaacagga
    22081 gtcctcgcat gaaatacatt ggagcgcacg ttagtgctgc tggcggtctg gcaaatgccg
    22141 caattcgcgc cgccgaaatc gacgcaaccg cgtttgcctt gttcaccaaa aaccaacgtc
    22201 agtggcgtgc cgcaccgctc acgacgcaaa ccatcgatga attcaaagcc gcctgtgaaa
    22261 aatatcacta cacatcggcg caaattcttc cccacgacag ttatctgatt aacctcggac
    22321 atccggttgc tgaagctctg gaaaaatcgc gcgatgcctt tatagatgaa atgcagcgtt
    22381 gcgaacagct ggggctttct ttgctcaact tccaccctgg cagccatctg atgcagattt
    22441 cagaagagga ttgccttgcg cgtattgccg aatccatcaa cattgtgctg gataaaactc
    22501 aaggtgtcac cgcggtgatt gaaaacaccg ccggtcaggg cagtaactta gggtttaaat
    22561 tcgaacatct cgcagcgatt atcgacggcg tggaagataa atcccgcgtc ggcgtctgca
    22621 ttgatacctg ccatgctttc gctgccgggt atgatttgcg tactcctgcc gaatgcgaga
    22681 aaacgttcgc tgattttgcc cgtattgttg gctttaagta tctgcgcggg atgcacctca
    22741 acgatgcgaa aagcaccttt ggcagccgcg ttgaccgtca tcatagcctc ggcgaaggca
    22801 atatcggtca tgatgcgttc cgctggatca tgcaagacga ccgtttcgac ggcattccgc
    22861 tgatccttga aaccatcaac ccggatatct gggcagaaga gatcgcctgg ctgaaagcgc
    22921 aacaaactga aaaagcggtg gcctgatcga tgaacgacag ggaaaagcag attcttaaaa
    22981 ttttacggcg taacccgctg attcaacaga atgaaatcgc cgatattctg caaatcagcc
    23041 gctcacgtgt tgccgctcat attatggatt tgatgcgcaa gggtttgatt aaaggcaaag
    23101 gatatatcct taccgagcag gattactgtg tggttgtggg tgcaatcaat atggatattc
    23161 gcggaatggc ggatatccgt tacccgcaag cagcctctca tccaggttct gttcactgtt
    23221 ccgcaggcgg cgtcggacgt aatatcgcgc ataacctggc tctgcttggt cgggatgtgc
    23281 atttaatttc agctattggc aacgattttt acggcgagac attactggaa gaaacgcgcc
    23341 gcgcgggcgt gaacgtttca aactgcattc gtctgcatgg acacagcact gcaacttatc
    23401 tcgctatcgc taataagcag gaagaaacta tcctggcaat caatgacaca catattctgc
    23461 aacagctaac gcctcaactg ctcaatactt cacgggactt gattcgccat gctggtgtgg
    23521 tgctggctga ttgtaatatc acacctgaag ccctggagtg ggtctttacc attgctgatg
    23581 aaattccgat gtttgtcgat accgtttccg aatttaaagc gaacaaagta aaaaactggt
    23641 acagccggat ccacactctg aaaccgacac aaaatgaact ggaaattttg tgggggcaac
    23701 cgattaaaga cgacaacgat cgcatcagag ctgtaaattc gctgcatcaa caaggtgtta
    23761 aacgcatttt tgtttatctg aaagatgaat ctgtattttg cagcgataag gatggagaac
    23821 aatttttgct gaccgcgcca gcgcatacga cagtagacag ttttggtgct gacgatggtt
    23881 ttatggcggg cctggtatat agctttcttg aaggatacag tttccgcgac agcgcccgtt
    23941 ttgcgatagc ctgcgcggca atttcgcgcg ccagcggcag cttaaacaac cctaccctgt
    24001 ctgccgataa cgcgctttca ttagtgccaa tggtgtaaca atgttgccgg attcgacgct
    24061 tgaccgcatc ttatccggct cataaattgc gcactacgct aaaccaataa agaatcctgc
    24121 aatagtagca ctcatcaggt tactgacagc aaaaacgcaa tagccagtaa taccaccatt
    24181 cccagaacac ttctcatgac atccatagtt attatctctt catgtcattt tgggcgacgg
    24241 ctctctgccc atcataaata gccgggcctg catcttgcga tactgacccg gcttgttatc
    24301 aacacttaat gggtttttat gtagccgcgt acgcactctt cgactaatcc ccagaaccag
    24361 tcggtatcaa tagtgatgcc gactttggta ttggcgggct tgccaagaac acccagctcg
    24421 tcgcagacgg tacgcccata gcaagggcca ctgttaacgt ccacttcgac gtacatctcc
    24481 tgggttttaa tgccatcagg gttaatcaaa taaccgatgc aggtggcgtc gtgcaccggg
    24541 ccgccagcca ggccgtagtt ttcgaactgc gttttgagag tgaagttcat gatgtcgctg
    24601 aacaactctc cggcagggcc gccagccctt tctatccgag caatcacatc cggggtgcaa
    24661 acggtctggt tggtaagatc gaggcctatc atcactaatg gaacgccgga ggtgaacacc
    24721 acgcgtgcgg cctccgggtc agcaaagatg ttgaactcag cagatggggt gaagttgcca
    24781 gtaccataag caccgcccat cagaacaatt tcacggattt tcggcaggat cgcgggttgc
    24841 atacgcattg ccaccgcgat gtttgacaac ggaccaaccg gcaccagagt gatatcgcca
    24901 tcgcttgcca taagggtatc gatgatatat ttcaccgcat gagtgttttc tgcctggcgg
    24961 gtcagcggct cgaataccgg gccatccagt ccggtttcac cgtgaatatt atcggcaaca
    25021 atttgttgac gcataatggg ctgcggcatc cccgcataaa ccggaacatt aatctccagt
    25081 ttctggcaaa cattcaggcc attaattaat gttttatcga gggtttgatt acccgctaca
    25141 atggtgatac ctaataaatc aatcgccgga tgtttcgccg ccatcattat agcaatagca
    25201 tcatcatgac ccggatcaca atccagaata atttttctct tttccattgt ttatttcctc
    25261 tgtttccagt tgcgttattg tttctacagc aaagaaaatt cgacctggcc gatgattgaa
    25321 tcttaacaac agcgtacgta tgctaaatat gagaaatctc atagcggata aacatcgtga
    25381 aagaaatcca caataatgat cttaagcagc aattgataag tgaatctgcg tttaaggatt
    25441 gcttttcaac ggatgtttca gccgatacgc ggctgtttca ttttttagcg cgtgactaca
    25501 ttgtgcagga agggcaacag ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc
    25561 tttatgtcac gcttgctaat ggtcgcgtgt cgctgatcga tttctttgcc gcgccctgtt
    25621 ttattggcga gattgagtta atcgacaaag accatgaacc gcgtgcagtg caggctattg
    25681 aagagtgttg gtgccttgcg ctccctatga agcattaccg cccgttgtta ttaaacgaca
    25741 cgctattttt acgaaaattc tgcgtcacct taagtcataa aaattatcgt aatattgttt
    25801 ctttaactca gaatcaatca tttccgttag tgaatcgcct ggcagctttt atattactct
    25861 cgcaggaagg tgatctttat cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt
    25921 cttatcgaca tcttttatat gttcttgcgc aattcattaa tgacggttta ttaattaaaa
    25981 gcaagaaagg gtatctcatt aaaaacagaa agcagttgtc aggactggcg ctggagatgg
    26041 acccggagaa taaattctcc gggatgatgc agtaaaaatt attggcaata gtgcgattgc
    26101 cggatgcaac gcttaataag gcgtacatcc ggcaatgaaa ctcaagctaa accaataaag
    26161 aacccggcaa tggtcgcgct catcaggttg gaaagcgtcg ccgccgccag cgcccgtaaa
    26221 ccaagctggg cgatttccgg cgcgcgtttt ggcgaaatag ccgaaaacgc tccaacgaca
    26281 acaccgatag aaccaaagtt tgcaaaccca cagagggcga acgagataat ggcaatggtc
    26341 ttgacatcca gcgcgccgct cgtttgcaga tagggcgaga aattgagata agcaacaaac
    26401 tcattaatcg ccagtttctg cccaatcaaa cttccggcaa gatttgcatc actccagtcc
    26461 acccccataa tccatgccaa cggggccagc acatagccga aaatgctttc cagagagaca
    26521 ttggcaaaac caaaccagcc gccaactcca ccaataatgc cgttgatcag cgcaatgatc
    26581 gccacaaatg ccattaccac cgtcgccaca ccggcggcga ttttcagccc ggtcattgca
    26641 ccgttcgccg cagcttcaat aatgcttttt ggcggtgttt cgctaaacga cagattttca
    26701 aacgtaactt gcgaaggttc cgtcgccgga ctaagaatac gtgcaaacag gataccgcca
    26761 ggaatagcca tcagcgaagc ggctaacaaa tagtcaatcg gcacgcccat tccggcatag
    26821 ccaatcatca tcgaacccgc aatagacgcc atgccgctac aaatagccgt aaatagctcg
    26881 ttgcgattca tgcgatcaat aaaaggttta acgatcgccg ggatctcatt ttgcccgagg
    26941 aaaatagtgg tgactgcgac aaaagattcg attttgctga tgttgagggc tttctgaaaa
    27001 atactgccaa gaatgcggat cagcagcccc attacgccaa tgtagtacag caagctgata
    27061 agcgcggtaa cgaaaataat cgccgggagt acgcgaaagg cgaagataaa gcccgcaccg
    27121 tcaaacagaa catccatttt gggaccgacc agcgaaccaa aaataaaggc acttcctgcg
    27181 tcactgtagg acatcacttt atggacgcct aatgccgcct gctctactgc ccattttccc
    27241 gggggaaaat agagcatgat gccaccaatg gcaatttgca gcagcagcgc agccccaacc
    27301 gtgcgcaagc tgatgctctt tttatttacc gacaacacaa aggcaattgc cagcaatacc
    27361 accatcccca caacacttct cattatatcc ataatgattt tcccttcatg ccagtaaacc
    27421 cggcgtcagc gccaggtttt ggtatgtttg atgagtacgg gcgacggctt tctgcccacc
    27481 agaaattaac ccacgagacg ctgatattct ttggcaattt cgctcgccag aatggcgtta
    27541 ttaaagacca gttgaatatt ggatttcagg ctatcaccgc cggttagctc cgcaacacgc
    27601 gccagcagga acggtgtact ctctttccca ataacaccct gttcttcggc ttcagcaacg
    27661 gcttgatcaa ttgctgcatt gatggtttct tctggcatcg caaactgttc cgggatcggg
    27721 ttcgctacca ccaggccgcc gttcaaaccg gtttgccact tcactgccat cgcgcgagca
    27781 atttcgcggg cactgtcgag acgaatgctg acttcaaatg ggctggttcg gcagaaaaac
    27841 gcaggcagcg cttttgtctg ataaccaatt aacggtacac cgaaggtttc taaatattct
    27901 gttgttaatc ctaaatcgag aatagatttt gcccccgcac aaacaacagt gacattggta
    27961 tttgccagtt cctgtaaatc ggcagaaata tcgaaggtat gttccgctcc gcgatgcaca
    28021 ccaccaatac ctccggttgc aaaaacttta atcccagcca gtgctgcaat aatcatggtt
    28081 gatgcaacgg tagtcgcgcc atttttccct gcagcaacga caaaaggtaa atcacgacga
    28141 ctaactttcg tcacgttatg tccttcacgg cccagcaatt cgatttcttc tttacttaag
    28201 cccactttca tcacaccgcc aataatggcg atagtggctg gtactgcgct ctgtttacga
    28261 attgtttctt ctacttcaat tgcggtttgg gcattttgtg ggaatggcat tccgtgagaa
    28321 attatggttg attccagcgc aacaaccggt tttttatttt ttaaagcatc ctgtacttca
    28381 gcggatattt gtaataattc aggggacaga gttaattcag acattctgtg ttctccacta
    28441 acgatgtaac attggcaatt gataattcag ggttgttggt atattcacag gcaagtgcca
    28501 ttgacgaaca tccctgcgca aatcgaacag aatcgataaa cggcataccg tctacccaac
    28561 aggaggccag ccccgccatc atggcgtcgc ctgccccagt aacattgatg acattcgttt
    28621 taataggggc cgaccaccca ctttcaccgt tgatatcact gtagtaaacg ccatcccctc
    28681 ccatgctcag caccaggcgg ttcaggccgt gttgatgaaa ccaggcggcg acctttgcga
    28741 catcttcacg tccggaaagt gcaattccgc tcaacgtttc ggcttcaaga cggttaggtt
    28801 ttaacgtatg gattttgctc agatgatcgc ggattttgac gcatttccat gccgataccg
    28861 gatcaataaa tacaggcgtt tccccgctat tttccagaac ccagaccagc gcctcttcac
    28921 taaggttgca atccgcgaca ataacctttg caccgcgaat aaactcctga tgctgggcaa
    28981 gaaattctac agaaatacag tcactaatac tcatatcatt tatcgcgaca agcatttctc
    29041 ctgtattatc gagcagtgat aaatagctgg atgtattctc acccggaaca attaagcatt
    29101 tatcgacata aacaccggat tgattagttt gcgccagtaa tgattgccca taaaaatcac
    29161 cacctactgc acttaaaagc caggcatttt tccccagtaa cgccagatta tgagcgatat
    29221 tccttcccac gccacctggg gtaaatttaa tttttccggg attcgaatcc gcataattca
    29281 atggatgatg tgaataccca gccacatcaa tattcgccga gccaataatt actatgtagt
    29341 ccttttcgcg catagcacgc cccctggcaa taaattgcca acgaagaaat tgggtaaaat
    29401 agtcattaca gtcaataaga ccattagagc acgtgttcag atttaaacac atgttcataa
    29461 taagcatgtt taacgcaatg taaatcgatc agaaaaaacg aaatcggcca gatatgaagg
    29521 cggtcacaaa atttgtctat ttatgcagca acaaaagaga aagttgtgat gtgtaacaca
    29581 caatatggca ggcaagaaaa agggcagaga tgtttctgcc ctcagattgt tgacaatgcg
    29641 cgctttattc atgctggatg tggcgtaaac gccttatccg acctacaaaa gcgcaaaaat
    29701 tcaatatatt gcagggttca tgtaggtctg ataagcatag cgcatcaggc aattttgcgt
    29761 ttgtcatcat cggggcagag atgtctcagc cccgatgatg aagagaaatt atgcgttttt
    29821 ttctactatt tgtgtttccg ggcgtttgag gaaggcatag gccaaacccg ccaccagcgt
    29881 accagcaata attgctacca ggtaacccaa taccggcgta atagcgccag ggatcagcag
    29941 aacaaacaga ccaccgtgtg gtgccatcag tttcgcacca atcgccattg agattgcgcc
    30001 agtcagcgcc ccaccaacga tacagcacgg tagcacacgc atcggatcac gagcagcaaa
    30061 cggaattgca ccttccgaaa taaagcacag accaagtacc agagcggctt tgccaccttc
    30121 ctgctgcgct ttgtcgaatt tgcgacgcgc caccattgtt gccagaccca ttgccagcgg
    30181 tggcaccata cctgccgcca taatcgccgc catcgggcca taggtttgag tactcagcag
    30241 acccacaccg aatgcgtacg ctgctttatt taccggaccg cccatgtcag tacacatcat
    30301 gccaccgagg atcgccccca gcagaacagc attcgcagtc cccatggtct gcagccagtg
    30361 agtcaaccca tcgagaatgc cagcaaccgg tttaccgatc aggtaaatca tcgccagacc
    30421 gaccaccaga ctggaaatta gcggaatgat caggatcggt ttcagtgcct ccatactctg
    30481 tggcagtttc agttgcgtac ttattaactt cgcaatgtaa cccgccagga agcccgcaat
    30541 aataccgcca atgaagccag aaccggtgct aaccgccagc ataccgccaa tcaggcccgg
    30601 cgtgagaccc ggacgatcgg caatggagaa ggcaatataa cctgccagta ccggcaccat
    30661 cagcgcaaag gctgaaccac caccaatctg catcagcgcc gctgccaacg tacccggctc
    30721 tttaaacgct tcgataccaa aagcaaaaga aagcgcgata cacagaccac ctgcaacaac
    30781 catcggcagc atgtaagaga cgcccgtcag caagtgacgg taagcacctg cactctcttt
    30841 cttaccttca gtggtcgctg tttgagtttt gcccgccggt tcatacggcg ttgcttcagc
    30901 aaccgcttta tccagttcct gcgcagtttt cttcagcgcc agaccggtag aggtgcgata
    30961 catcggttta ccagcaaatt tcgccagatc cacttcgata tctgccgcca caatcaccag
    31021 atccgctgct gcgacttctt caggggtgat tgcattgccc gcgccaacag aaccacgggt
    31081 ttcaactttc acccaccagc cacgtttttt cgcttcggtt tcaatggctt cagccgccat
    31141 aaaggtgtgt gctacgccag tcgggcaagc agtcaccgca actacgcgtt tcggaccgct
    31201 ggcagcaacc ggtgctgtcg cagcgaccgg cgcagtgtaa ggtttcgcat ggcctttggc
    31261 ttcactcagg aacaactcag ggtgcgcaac tgcccgggaa atatcaccca gccagacatt
    31321 tttaccgttc agcgcgctgt cattcgggat ggaatcaccg agaacaatcg ccatttctgc
    31381 ttcgttcgga ttgtcgatga tttccagttt tgcttttcgc gccgccgcgc ccagcagggt
    31441 cttcgccata taggcgcgtg cctgaccgag attagcgtca ataatcagca gcgttttcat
    31501 tatgcctctc ctgctgtcag ttaaaaggtt gtaagtcgac gcgcgccatc attgcggcca
    31561 actgcggacg atcggtaata cccacattgc tttgacttac cgccagggct gcaacagctg
    31621 tcgccagacg cagtgtgtgt tcactggatt cacgcatcag caagccataa atcaggccac
    31681 caaccataga atcccctgcg ccaacggtgc ttacgacatc gactgacggt ggtttggcga
    31741 tccattcgcc ggaggcatta acccaaagcg cgccttcggc acccagtgaa ataacaacat
    31801 gcgcgatgcc ttgttcacgc agcgcatgcg cagcttcaat cacatctttc atttcaggca
    31861 gtttacggcc tgcccagatt tccagctcgc ggcggttagg tttcaccagc cacggtgccg
    31921 ctttcaaacc tgctactaac gcttcacggc tactatcaaa gataatgcaa ggacactgac
    31981 tacgcaggcg agtcatccag tcggtgaacg cttccgggct gacgcctgac ggtaagcttc
    32041 cgctgacaca gaccatatcg aactgaccga gccagctcag agaatcagtc acaaagcgtt
    32101 cccagtcggc gggggtgact tcaaaacccg agaagttgaa gtcggtcact tcgccgtctt
    32161 tttccgtcag cttaacgtta attcgggtgc gcccctgtac aacctggaaa cggttggcaa
    32221 tgcccagctc gctgaacagt tgctgaaaac catcctgatt gtctttaccc aggaagccgc
    32281 caacggtgac atcaattccc aggtctttta atactttggc cacgttgatg cctttacccg
    32341 ccgcatgcag accagtggtt ttcaccaggt tcacttcgcc gcgttcaatt tccgggcaga
    32401 aaccaacaag gtcataagcc ggattaaggg tgatcgtagc aacacgtctg ctcattatgc
    32461 gccctcccca agaccagcag cgatagcgtc gccaatggct ttcagcgcct gttcagcatc
    32521 tgcaccctgg gcggtaaagc gtaggcgatg acctttctta acgccaagtg ccacaacttt
    32581 catcagacta cgtccgtttg ccggtttgcc ggtgccatca aggtttgtca cggtaatatc
    32641 actgttaaat tgtttaatgg tattgaccag catggtacct ggacgagcat gcaggccgtg
    32701 ttcattgcgc accacaaact ccgcgctcaa cacgtcgtcg gtcggcgcat catcgctggt
    32761 cagcagcgcc agcaacgttg ccgcatccgc tttcagcaag cggtcagctt tattatcgag
    32821 caacaaatca gcgagacgct taagaaccgc gatgggctga tcgtcattca tcgccacact
    32881 caccagcatg gctgccgttt cgccgtccac atcaaaagca tttgccgcac ggcttaccgc
    32941 aatcgcgcta cgcagattgc cttcggcgct atcgctcagc cagataccct gcccgaggtt
    33001 cagcggttgt tcattgatgg ctttggtgac gaaagtggcg tcaactgccc ccgcctcttt
    33061 cagacgcgca gcgttcagcg cctgaagagt cagcagatcg ctggcgacga tatccaacgt
    33121 cagcatttcg ttgtcgagct tcagctgctc actctgcttt tcgcccatca gcaatgcgcg
    33181 aagttcttct gctgttgttg ctgacttcag ttgttcagca acggaatcat cgctcagtac
    33241 gtgggtcagc tggcgtagca ggcccagatg ttcatccgag ctggcagcaa taccgatcgc
    33301 cacgtacgct acctggccgt caccccaggt gacgccttcc gggaactgaa atacctgaac
    33361 gccggttttc agcacctgat cgcgggtgtc ggtagtgccg tgtggaatag caataccatt
    33421 gccgaggaac gttgaggttt gctgttcgcg cgccagcatg ccattgacgt agccttcagc
    33481 tacattaccg gcctgcacca gcgccgcagc gacctggcga atcgcctctt ctttgtctcc
    33541 ggccttttcg cccggatgga tgtcctgtac ggataactgg aacatagttc tcctctcttg
    33601 ctgaattgaa acgattcagc ttctatgaga aaaaagcgcc aacctggctt agggttaaag
    33661 acaagatcgc gctgaaacgt ttcaagaaag cataatactt ttgtttcagc acgcaaggaa
    33721 agccgtaaag ttatgagcaa aagtttgatg tgctgcacaa ttttttgtca tttttcctaa
    33781 ttgctgatgg gaaaatccgt tatcagcaat ttcatttcag cagcgtcagc agccaatcag
    33841 caacagtgaa atggcatttt gattattcgt ggcgcttttg atttcgctgt ctgtttattt
    33901 tctgacaagc agcgtaaact ccgcgtcttc ctcttccagt gatcgaccag catgcataac
    33961 tcccccgcag tcaccagcgc gaaatcgttt gacctgacct cgatggcgtt tttaatcgtt
    34021 gcctttctca ccggtattgc gggcgctctg caaaccccga cactcagtat ttttcttact
    34081 gatgaagtac atgcccgtcc ggcgatggtg ggattcttct ttaccggcag cgcagtcatt
    34141 gggattctgg tgagtcagtt tctcgccggg cgctctgata agcgcggcga tcgcaaatcg
    34201 ctgattgtct tttgctgcct gttaggcgtg ctggcctgca ccctttttgc ctggaatcgc
    34261 aactactttg ttttgctatt cgttggcgtc tttcttagca gctttggctc aaccgctaac
    34321 ccgcaaatgt ttgcccttgc ccgtgaacat gccgacaaaa ccggacgtga ggcggtgatg
    34381 ttcagctctt ttttacgcgc tcaggtttca ctggcatggg tcattggccc accgctggct
    34441 tatgccttag cgatgggttt cagctttacg gtaatgtatc tgagcgcagc ggtagcgttt
    34501 attgtttgcg gcgtgatggt gtggctgttt ttaccgtcga tgcaaaaaga gcttccgctg
    34561 gcgaccggca cggttgaagc gccgcgccgt aaccgtcgcg atacgttgct gctgtttgtc
    34621 atttgtacat tgatgtgggg ctcgaacagc ctgtacatca tcaacatgcc gctatttatt
    34681 atcaacgaac tgcatcttcc cgagaaactg gccggtgtga tgatggggac cgccgccggg
    34741 ctggaaatcc cgaccatgtt gattgccgga tattttgcca aacgtctggg taagcgcttc
    34801 ttaatgcgcg ttgctgccgt gggtggcgtc tgtttttacg caggaatgct gatggcgcat
    34861 tcacctgtca ttctgttggg cttgcagctg ctaaatgcta tttttattgg cattctgggc
    34921 ggtatcggga tgctctattt tcaggatttg atgcccggtc aggcaggttc agccaccacg
    34981 ctctatacca acacgtcgcg cgtgggctgg atcatcgcag gatcagtggc gggcatcgtc
    35041 gccgagatct ggaattatca cgctgtgttc tggtttgcga tggtgatgat tatcgccact
    35101 ctgttttgct tactgcgaat taaagatgtt taaggcgcgg tcaacatttc cagatcgata
    35161 agccaggtca ttgcctgctg acgcgagtta ccgcacattt cagcgctggc ttgtaagcct
    35221 gcacagactt tcggtcgcag tggcgaggtg aaaattttac agcgttgctg ttcatcaagc
    35281 tgaatgcagg gcgtattggc gggcttgcca tctggcatac cgggaatagg gctggaaatt
    35341 gaaggggcag tgcaacacgc cccgcaaccc ggacggcatt ccatacgttg ttctcttaag
    35401 gttaaccacc ggattgatga gcgcgcacag taccaccttt tttgcaccag caaaagtgcg
    35461 aataccactt gcccgaaaga cccgtcgcga gtactttgtc gcgatatttt tgacattttc
    35521 gactacagga atttttcgat gccaagagcg aacgaaatta aaaaaggtat ggtactgaat
    35581 tacaacggca aactgctgct ggttaaggac attgatattc agtcgcccac tgcccgcggt
    35641 gccgctacgc tgtacaaaat gcgtttttct gatgtccgta ccgggctgaa agtagaagag
    35701 cgtttcaaag gtgatgatat cgttgacacc gtgacgctga cccgccgtta cgttgacttc
    35761 tcctatgtcg atggtaacga atatgtcttt atggataaag aagactatac cccgtatacc
    35821 ttcaccaaag atcagattga agaagagttg ctgtttatgc ctgagggcgg catgccggac
    35881 atgcaggtgc tgacctggga cggtcaattg ctggcgcttg agctgccgca gaccgttgat
    35941 ctggaaatcg ttgaaactgc accaggcatc aaaggggcat ctgccagcgc ccgtaacaaa
    36001 ccggcgacat tgagcactgg tctggtgatt caggtaccag aatacttaag cccgggcgaa
    36061 aaaattcgta tccatatcga agaacgccgt tatatgggtc gtgctgacta acttcagccg
    36121 catgcagaaa agggatagct caggctgtcc cttttttaat ttattatacc aatcttctat
    36181 tttgcgcttc ctgaacacct tacgcccgca attttcactc gttgattgat cgccctcaca
    36241 cttcatcgca ttaacaatcc agaccaattt caattgttgt catataactt tacactgtca
    36301 ttgttaatta atcgttacta agacgtgact cctatgaata caatcgcctc cgttacgctc
    36361 ccgcatcatg tacacgctcc acgctacgat cgacagcagt tgcaatcacg tatcgttcat
    36421 tttggctttg gagcctttca ccgcgcacat caggcattac tgaccgatcg tgtgctgaat
    36481 gcccagggcg gcgactgggg gatctgtgaa atcagcttgt tcagcggcga tcaactgatg
    36541 agccaactcc gcgcacagaa ccatttatat accgtgctgg agaaaggtgc ggacggcaat
    36601 caggcgataa ttgtcggtgc cgttcacgaa tgtctgaatg caaaactgga ttccttagcg
    36661 gcaattattg agaaattttg cgagccacag gtggcaattg tttccctgac gattaccgaa
    36721 aaaggctatt gtattgaccc ggccaccggt gcactcgaca ccagcaatcc gcgtattatt
    36781 cacgatctac aaaaccctga agaacctcac tccgcgccag gtattctcgt cgaagcactg
    36841 aaacgccgcc gtgagcgcgg ccttacaccg tttaccgtgc tctcctgcga caatattccc
    36901 aacaatggcc atgtggtgaa aaacgcggtg ctgggaatgg cagaaaaacg ttcgccagaa
    36961 ctcgccgggt ggataaaaga acacgtcagt tttccgggaa ccatggtcga ccgcattgtt
    37021 ccggctgcaa ccaacgaatc actggcggaa atcagccagc atctgggggt gaatgatccc
    37081 tgcgcgatta gctgcgaacc gtttatccag tgggtggttg aagataactt cgtcgctggg
    37141 cgtcctgcct gggaagtcgc aggtgtacaa atggtgaatg atgtcctgcc atgggaagag
    37201 atgaaactgc gtatgcttaa tggcagccac tcttttctcg cttatctggg ttacctctca
    37261 ggattcgccc atatcagtga ttgcatgcag gatcgcgcat ttcgccatgc cgccagaaca
    37321 ttaatgctgg atgagcaagc gccgacactg cggattaaag atgtcgattt aacacaatat
    37381 gcggataagt taattgcacg ttttgctaat ccggcactga aacataagac ctgacaaatc
    37441 gcgatggatg gcagccagaa attaccgcaa cgcatgttgg caggtattcg catacatctg
    37501 gggcgcgaaa cggactggtc gttgctggca ttaggcgttg caggctggat gcgttacgtc
    37561 agcggcgttg atgatgccgg aaatgccatt gatgttcgcg atccgcttag cgataaaatt
    37621 cgtgaacttg ttgcggtcag cagcagtgaa caacgcgtaa ccgccctgct ttccctgcgt
    37681 gaaattttcg gtgatgatct gccagataac ccgcattttg tgcaggccat cgaacaagcc
    37741 tggcaacaaa tcgcacaatt cggcgcgcat caggcgctat taaacaccct caaaatttaa
    37801 cgatttctgc ggttaaagcg gatgaagctc gccttcgtcc gctctcccct tctcttttct
    37861 gcctttttta gccaggatta acgctcagtt aacttaccag agtattgcgg agccgttgtg
    37921 accagaacca acctcatcac cggttttctc ggcagcggga aaactacgtc gattcttcat
    37981 ctgttagccc ataaagatcc caacgaaaaa tgggcggtac tggttaatga atttggggaa
    38041 gtcggaattg atggtgcttt gctcgccgat agcggcgcac tgctgaaaga gatccccggc
    38101 ggttgcatgt gctgcgttaa tggtttaccc atgcaggtag ggttgaatac cttactgcgt
    38161 cagggaaaac cagaccgctt gttgatagag ccgaccgggc tgggccatcc gaaacagatc
    38221 ctcgatctct taaccgcacc cgtctatgaa ccgtggatag atctgcgcgc cacgttgtgc
    38281 attctcgatc cacgcctgtt gctggacgaa aaaagcgcca gcaatgaaaa cttccgtgac
    38341 cagctggctg ccgcagacat tattgtcgcc aataaatccg accgtgcgac gcccgaaagt
    38401 gagcaagcgc tagagcgctg gtggcagcaa aatggtggcg atcggcaatt aattcacagc
    38461 gagcatggaa aaattgacgg tcatcttctg gatttgccgc gtcgcaattt agccgagttg
    38521 cccgccagcg ccgcgcattc tcatcagtat agcgtgaaaa aagggttagc agcgttaagc
    38581 ctgccagagc atcaacgctg gcgtcgcagt ctgaacagcg gacaagggca tcaggcctgt
    38641 ggctggatat tcgatgctga tacggtattc aacaccattg gtattctgga atgggcgcga
    38701 cttgcaccgg tggaacgcgt caaaggcgtg ctgcgtattc ccgaagggct ggtgcgaatc
    38761 aaccgtcagg gcgatgacct gcacattgaa acgcaaaacg ttgcgccacc ggacagtcgt
    38821 attgagctga tttccagcag cgaagctgac tggaatgcct tgcagagcgc gctgttgaag
    38881 cttcgtttag cgactaccgc gtaaggttgc ctgcgttttt cagtaagata attagagaaa
    38941 atatgattaa aaatttgccg caaatagtgt tgttgaatat tgtcggcctc gcgctgtttc
    39001 tttcctggta tattcccatt aatcatggat tctggttgcc gattgatgcg gatatttttt
    39061 atttctttaa tcagaaactg gtcgaaagta aggccttttt atggctggtt gcattgacca
    39121 acaatcgcgc tttcgacggt tgttcactgc tggcgatggg aatgttgatg ctgagtttct
    39181 ggctgaaaga aaacgcccca ggcagacgac gtatcgtgat tattggtctg gtcatgctat
    39241 taactgcagt ggtattaaac cagctgggtc aggcactaat tcctgtaaaa cgggccagcc
    39301 caacgttgac ttttaccaat attaaccgcg tcagcgaact gctctctgtt cccacgaaag
    39361 atgcctcacg agatagcttt cccggcgatc acggtatgat gctgcttatt ttttcggcat
    39421 tcatgtggcg ttatttcggc aaagttgcag gccttatcgc ccttattatt tttgtggttt
    39481 ttgcgtttcc cagagtaatg attggcgcac actggtttac tgacatcatc gtcggttcca
    39541 tgaccgtgat attgatcggt ttgccctggg tgttgctgac gccattaagt gatcgattaa
    39601 tcaccttttt tgacaaatca cttcctggaa aaaacaaaca tttccaaaac aaataactca
    39661 cagtaattaa catcatcagg tttattttta tagtgaggat aatcctgatg atgcgcctcc
    39721 tgctttcatc catcgagcgc aaaaatcatt ctctaagtaa atgaatggat tgcatgcgtt
    39781 tcactcaatc gtactttaat tgaccaatcc cgctgattaa ctttctgtat cactttttct
    39841 tataaaaaat catgtaaaac cgctcgccaa gaccgcacca atcgggtaat ctcgaactcg
    39901 ttttgcctcg gcggtagatt atcctcacag catataattt tgtgcgttag tccacagatt
    39961 tggccttaag gaattgtttc aacatgccca ggtaattagt ctcgtgtcgc ttggcatttt
    40021 tttataacga tatttgtcgt taaggacttc aagggaaaac aaacaacatg gtcaaatctc
    40081 aaccgatttt gagatatatc ttgcgcggga tccccgcgat tgcagtagcg gttctgcttt
    40141 ctgcatgtag tgcaaataac accgcaaaga atatgcatcc tgagacacgt gcagtgggta
    40201 gtgaaacatc atcactgcaa gcttctcagg atgaatttga aaacctggtt cgtaatgtcg
    40261 acgtaaaatc gcgaattatg gatcagtatg ctgactggaa aggcgtacgc tatcgtctgg
    40321 gcggcagcac taaaaaaggt atcgattgtt ctggtttcgt acagcgtaca ttccgtgagc
    40381 aatttggctt agaacttccg cgttcgactt atgaacagca ggaaatgggt aaatctgttt
    40441 cccgcagtaa tttgcgtacg ggtgatttag ttctgttccg tgccggttca acgggacgcc
    40501 atgtcggtat ttatatcggc aacaaccagt ttgtccatgc ttccaccagc agtggtgtta
    40561 ttatttccag catgaatgaa ccatactgga agaagcgtta caacgaagca cgccgggttc
    40621 tcagccgcag ctaataaacc gtttggatgc aatcccttgg ctatcctgac gagttaactg
    40681 aaagcactgc ttaggcagtg cttttttgtt ttcattcatc agagaaaatg atgtttccgc
    40741 gtcttgatcc aggctatagt ccggtcattg ttatctttta aatgttgtcg taatttcagg
    40801 aaattaacgg aatcatgttt atacgcgctc ccaattctgg acgtaagctc ctgcttacct
    40861 gcattgttgc aggcgtgatg attgcgatac tggtgagctg ccttcagttt ttagtggcct
    40921 ggcataagca cgaagtcaaa tacgacacac tgattaccga cgtacaaaag tatctcgata
    40981 cctattttgc cgaccttaaa tccactactg accggctcca gccgctgacc ttagatacct
    41041 gccagcaggc taaccccgaa ctgaccgccc gcgcggcgtt tagcatgaat gttcgtacgt
    41101 ttgtgctggt aaaagataaa aaaacattct gttcatctgc aactggtgag atggacattc
    41161 cactcaatga attgattccg gcgctcgaca ttaataaaaa cgtcgatatg gcgatcttac
    41221 ccggcacgcc gatggtgccg aacaaacccg caatcgtcat ctggtatcgc aaccctttgc
    41281 tgaaaaatag cggcgtgttt gccgctctga atctcaacct gacgccttca ctcttttata
    41341 gttcacggca ggaagattac gatggcctcg ccctcattat tggtaatact gcgctatcta
    41401 ccttttcttc acgtttgatg aacgttaatg aattaaccga catgccggtc cgtgaaacta
    41461 aaattgcggg cattcctctg accgttcggc tttatgcaga tgactggaca tggaacgatg
    41521 tgtggtacgc atttttactg ggtggcatga gtggaacttt cgttggactt ctctgctatt
    41581 acctgatgag tgtgcgtatg cgcccaggca gagaaatcat gaccgccatc aagcgcgaac
    41641 aattttacgt ggtgtatcaa ccggtggttg atacacaagc tttgcgggta acgggcctgg
    41701 aagtactgct acgctggcgg catccagtag caggagaaat tcccccggat gccttcatta
    41761 actttgccga agcgcaaaag atgattgtgc cactgactca gcacctgttt gagttgattg
    41821 cccgcgatgc cgcagaatta gaaaaagtac tgccggtagg cgtcaaattt ggcattaaca
    41881 ttgcgccggc ccacttgcac agcgaaagct ttaaagcaga tatccagaaa ctgctcactt
    41941 ccctgcccgc acaccatttc cagattgtgc tggaaattac cgagcgcgat atgctgaaag
    42001 agcgagaagc cacacaactc ttcgcctggc tgcactcggt cggcgtagaa attgctattg
    42061 atgacttcgg caccgggcac agcgcgctta tctatcttga gcgttttacg ctcgattatc
    42121 tgaaaattga tcgtggattt atcaacgcca tcggtacgga aacgatcact tcacccgtac
    42181 ttgacgcggt gctgacgctg gcgaaacgtc tcaatatgct gacagttgct gaaggggtcg
    42241 aaacgccaga acaggcacga tggctaagcg aacgcggcgt taatttcatg caaggctact
    42301 ggattagtcg cccgttaccg ctggacgatt ttgttcgctg gctgaagaaa ccgtatacgc
    42361 cgtagtggta aggtgtgctt acgtccctta ttattcatag tgaaagcatg ccggattgcg
    42421 gctaatgatg agtaaaagga aatccgttgc agatgattgt gcgcatactg ctgctgttta
    42481 tcgctctgtt cacctttggt gcgcaggcgc aggctatcaa ggaaagctat gcctttgccg
    42541 tactgggcga accccggtac gcatttaatt tcaaccattt tgattatgtg aaccccgccg
    42601 cgccaaaagg tgggcaaata acgttgtctg ccctcggcac cttcgataat ttcaaccgct
    42661 atgcactgcg cggcaatccg ggcgcacgca ccgagcagct gtacgacacg ctatttacga
    42721 cttccgatga cgaaccaggc agttattacc cgctgattgc tgaaagcgca cgctatgctg
    42781 acgattattc ctgggtggaa gtcgctatta atccacgcgc ccgttttcat gatggttcgc
    42841 ccattactgc ccgcgatgta gagtttactt ttcaaaaatt tatgaccgaa ggcttgccgc
    42901 aatttcgtct ggtctacaaa ggcaccaccg tcaaagccat tgcgccgtta accgtgcgca
    42961 ttgagttagc taaacccggc aaagaagata tgctgagtct gttttcgctg ccggtatttc
    43021 cagaaaagta ctcgaaagat cacaaactta gcgacccgct cgccacgcct ccgcttgcca
    43081 gtggtccgta ccgcattacg tactggaaaa tggggcaaaa tattgtctat tcccgcgtga
    43141 aagattactg ggcagcaaac ttaccggtaa accgtggacg ctggaatttc gacaccattc
    43201 gctacgatta ttacctcgat gataatgtcg cctttgaagc gtttaaagca ggtgcctttg
    43261 atttgcgtat ggaaaacgac gccaaaaact gggccacgcg ctataccggt aaaaatttcg
    43321 ataaaaaata catcatcaaa gatgagcaaa agaacgaatc agcccaggat acgcgctggc
    43381 tggcgtttaa tatccaacgt ccggtattca gcgatcgccg ggtgcgggaa gcgatcactc
    43441 tcgcctttga ctttgaatgg atgaacaaag cgttgtttta caatgcctgg agtcgcacga
    43501 acagttattt tcagaatacc gaatatgcgg ccagaaatta ccccgacgcc gcggaactgg
    43561 tgcttctggc accaatgaaa aaagatctac cgccagaagt cttcacgcaa atctaccagc
    43621 cgccggtatc caaaggcgat ggctacgatc gtgacaacct gttaaaagcc gacaaacttc
    43681 tcaacgaagc aggctgggtg ctgaagggtc agcaacgcgt taatgtcaca acgggtcagc
    43741 cactcagctt tgaattattg cttcccgcaa gcagcaatag tcagtgggta ttgccgttcc
    43801 agcacagcct gcaacggctg ggtatcaaca tggacattcg caaggtggat aactcgcaaa
    43861 tcaccaaccg catgcgcagt cgcgactatg acatgatgcc gcgcgtatgg cgggcgatgc
    43921 cgtggcccag ttccgattta caaatttcct ggtcatcgga atatatcaat tccacttata
    43981 atgcccccgg cgtgcaaagc ccggttatcg actcgctgat caaccaaatt attgccgcgc
    44041 agggaaataa agaaaaatta ctgccgttgg gacgagcact ggatcgcgta ttaacgtgga
    44101 attattacat gctgccaatg tggtacatgg cggaagaccg tctcgcctgg tgggataaat
    44161 tctcccaacc cgctgtacgc cctgtttaca gcctgggtat tgatacttgg tggtatgacg
    44221 ttaataaagc gacgaaactg ccgtcagcca gacaacaggg agagtagatg ggcgcttatc
    44281 tgattcgccg tctgttgctg gtgatcccaa cgttatgggc gattatcact atcaactttt
    44341 tcatcgtgca aattgcgcct ggcggtccgg tcgaccaggc catcgccgcc attgagtttg
    44401 gtaatgccgg agtattaccc ggcgcaggcg gtgaaggtgt tcgcgccagc catgcgcaaa
    44461 cgggcgtcgg caatatcagc gacagtaatt accgtggcgg acgcggatta gatccagaag
    44521 tgatcgccga gatcactcat cgctacggtt tcgataagcc gatccacgaa cgttacttca
    44581 aaatgctctg ggactacatc cgttttgatt ttggcgacag cctttttcgc agcgcctcgg
    44641 tgctgacgct gattaaagac agtctgccgg tctccatcac cctcggattg tggagcacgc
    44701 tgattatcta tctggtgtcg attccgttag gcattcgcaa agctgtttat aatgggagcc
    44761 gttttgacgt ctggagtagt gcatttatca tcatcggcta cgccattccg gcctttttgt
    44821 ttgccatcct gctgattgtc ttcttcgcgg gcggcagcta tttcgacctg ttccctctgc
    44881 gcggcctggt ttccgctaac tttgattcgc tgccgtggta tcagaaaatc accgattatc
    44941 tgtggcatat cacgctgccg gtgctggcga cagtgattgg cggctttgcg gctctgacca
    45001 tgctgacaaa aaactcattc cttgatgaag tgcgtaagca atacgtggtg acggcacgtg
    45061 cgaaaggggt aagtgaaaaa aatattctct ggaaacatgt gttccgcaac gccatgctgc
    45121 tggtgattgc cggttttccg gcgacgttta tcagcatgtt ttttaccggc tcgctgctga
    45181 ttgaggtgat gttttcactc aatggtcttg gcttactggg ctacgaagcg accgtctcgc
    45241 gcgattatcc tgtaatgttt ggtaccttgt atattttcac cctgattggc ctgctgctga
    45301 atattgtcag tgatatcagc tatacgctgg tcgatccgcg tatagacttt gagggacgtt
    45361 aatgtcgcga ctcagccccg tcaatcaggc ccgttgggcg cgttttcgcc ataaccgtcg
    45421 cggctactgg tcgttatgga ttttcctcct cttgtttggt ttgagtttgt gttctgaact
    45481 tatcgccaac gataaaccgt tgctggtgcg ttatgacggc agttggtatt tcccgttgtt
    45541 gaaaaactac agcgaaagcg attttggcgg cccgctggca agtcaggctg attatcagga
    45601 cccgtggctg aaacaacgac tggaaaataa cggctgggta ctgtgggcac cgattcgctt
    45661 tggtgctacc agtatcaact ttgctaccga taagcccttc ccttctccac cctcccggca
    45721 aaactggctg ggaacggatg ccaatggcgg cgatgtgctg gcgcgcattc tctatggcac
    45781 gcggatctca gttctgtttg gcctgatgct gaccctctgc tccagcgtga tgggcgtgct
    45841 ggcgggggcg ctacaaggct attacggtgg taaagtcgat ctctgggggc aacgttttat
    45901 tgaagtatgg tcggggatgc cgacgctgtt tttgattatt ttactttcca gcgtcgtaca
    45961 gcctaacttc tggtggctgc tggcaattac cgtcttgttt ggctggatga gtctggtcgg
    46021 cgtggtgcgg gcggagtttt tacgtacccg taatttcgac tacatccgcg cggcgcaggc
    46081 gcttggcgtc agcgatcgca gtatcatcct gcgtcatatg ttgccaaatg ccatggtcgc
    46141 taccctcact tttttaccgt ttattttatg tagttcgata accaccctga cctcactcga
    46201 tttcctcggc ttcggtctgc cgctcggttc accgtcactc ggcgaactgc tgttacaagg
    46261 gaaaaataac cttcaggccc cgtggcttgg gatcaccgcc ttcttgtcgg tggcgatatt
    46321 gttgtctttg ctgatcttta ttggtgaagc cgtccgcgac gcatttgatc ctaataaggc
    46381 ggtgtagcat gacgcaaact ctgttatcga ttgaaaattt gtcggtgggt tttcgccatc
    46441 agcaaaccgt acgtacagta gtaaatgatg tttcactaca gattgaggct ggcgaaacgc
    46501 tggcgctggt aggtgagtct ggttcaggta agagcgttac cgcgctgtca attttacgcc
    46561 tgctcccttc cccgccggtt gaatatctct ccggcgatat tcgttttcat ggcgaatcgc
    46621 tgcttcatgc cagcgatcaa acgttacgcg gtgtacgcgg taataagatt gccatgattt
    46681 ttcaggaacc gatggtgtcg ttaaatccat tgcataccct ggaaaaacag ctttatgaag
    46741 tgctttcact ccaccgcggg atgcgtcggg aagcggctcg tggcgaaatt cttaactgcc
    46801 ttgatcgcgt cggtatccgc caggcggcaa aacggctgac agattatccg catcagctct
    46861 ccggcggcga acgccagcgg gtgatgattg cgatggcgct gttaacgcga ccggaattat
    46921 taattgccga tgaaccaacc accgcgctgg acgtctctgt ccaggcgcag attttacagc
    46981 tgttacgcga actgcaaggc gagctgaata tgggtatgct gtttattact cataacctca
    47041 gcattgtcag aaaactggcc caccgcgtgg cggtaatgca aaacggtcgc tgtgtcgagc
    47101 aaaataacgc cgctacgcta tttgcctcac ccactcatcc ttacacacaa aagctactca
    47161 acagtgaacc gtcaggcgac ccggtcccat tgccagaacc tgcctcaacg ttgctagatg
    47221 ttgaacagct tcaggttgcc ttccccattc gcaaagggat tttgaagcgc attgtggatc
    47281 atcatgtggt ggtgaaaaac atcagtttta cgctacgggc gggtgaaaca ctgggtttag
    47341 tgggcgagtc cggttccggg aaaagcacga cgggactggc gctgctgcga ctgattaatt
    47401 ctcatggcag catcgtcttt gacggtcagc cactgcaaaa tttaaatcgc cgccagctgt
    47461 tacctattcg tcatcgcatt caggtggtat ttcaggatcc aaactcctca ctcaacccac
    47521 gactcaacgt tttgcaaatt attgaggaag gcttacgggt tcaccagccg acgctttctg
    47581 ccgcacaacg cgaacaacaa gtgatagccg tgatgcatga agtgggatta gatcctgaaa
    47641 cacgccaccg ttatccggcg gagttctctg gtggtcagcg gcaacgtatt gcgattgcca
    47701 gggcgttaat tcttaagccc tcgctgatca ttcttgatga accaacatca tcactcgaca
    47761 aaacggtgca ggcgcaaata ttgacgctat tgaaatcatt gcaacaaaag catcaactgg
    47821 cctatttgtt tatcagccac gatttgcacg ttgtccgcgc gttatgtcat caggttatcg
    47881 tactgcgaca aggggaagta gtggaacaag gaccgtgcgc gcgcgtgttt gccgcaccgc
    47941 agcaggaata tacgcgtcag ctactggcgt tgagctgacg cttaaaaagg attgtagttt
    48001 gaaaagggtt cggcaattgc cacaccaaaa tttttcaatc ggcatgttgc agcaaattcg
    48061 tcctggcgat ttacaaacag gcacggctca ccttcacact ccagcaccga acatggaact
    48121 tcgatgtcgc ttaacgcctg gcttagtttg tacatcaccg accatgcatt atcaccatcc
    48181 gggctaagca atttaagcgc caccaggctg ttgtcaccgc acggtccatt ttgcggaatc
    48241 ggttcaacct tcgaacctgc gaaaccagca gaccagcgat agttctgcgg cagtcgatgg
    48301 acgattgaga gttgtaatga agtcacttat tttccccgga agtacatttt acttcacaat
    48361 ttgtttacat caattttaac acatcatcaa caatccgtct ttatacagat cgtaaagatt
    48421 gatgcctcgc ctgcgtggcc tctatggctc tgatttaaat aatatctgcg ggcgtggtca
    48481 cgtttgcggg gctatatgta cccgtttctg cgatctaact caaccttttt aactacaatg
    48541 atgtgacttt ttacataaat tgattttaca taaaacaaac atatatcggg ggaatgatag
    48601 atttgtggtt gacatgcatc aacaaaggaa gccttttagc ttcctcgttg tgcaatagat
    48661 caccgttttt tcggccgact ggcgtacaaa cagaagagaa tggagctggt tgcgcagaat
    48721 gcaattgacc aaatcatcgg ccatgcagag ttaaaggtcg caagagaaag taatgcgcca
    48781 acaattgccc ctatgccgaa acggaaggtt cctgccagcg aagatgccgt tcccgccata
    48841 tggggaaact catcaagaat gaccgccatc gcattggatg acaccatcga cacgcagccc
    48901 acaaacgccg caacgccaac caccagcgac cagaatccca gccccagcag tgcactgatg
    48961 accatccacg cagccataat aaactgtatc cacaaccccg agcggaacat atttaacgcg
    49021 ccaatacggc ggacgaagcg gctgttaaag atggtcatca cgaacagaaa aagaatgttt
    49081 agcgcaaagt aataaccaaa gttttccggc gcgacgtggt tgatttcaat gtagacaaac
    49141 ggtccggcgc ttaagaatga gaacatcccg gcaaagctga aaccgctggc cagcatataa
    49201 ctcaggacac gcttatgacg gaacagcgcc gcaaagttac caatagtggt acgaatgtga
    49261 aaaggctggc gacgctccgg cggtaaggtt tctttgatca gaaagaagat cattgccgaa
    49321 gccagaatcg ccgccacggc gaggatccag aagatgtaat gccagctcag ccacaccagc
    49381 acccagccgc caactatcgg tgccatcagc ggtgcaatgg tcgtcaccag catgacaaac
    49441 gacatcatcc gtgagaactc ttctttcggg tagatatcac gcatcaaagc gttgataacc
    49501 acgctggccg cagccgcagc cagcccgtgg aagaaacgca tcacaatcag ctgatcgatg
    49561 gtttgcgcca acgcacacgc caccgcggcg gcggcaaaca ccagcgtacc gccaagaacc
    49621 accggcttac gcccgaagct gtctgccatc ggcccgtaga ttaactgccc caacgcaaag
    49681 cccagaatat aagtactcag ggtcatctgc gtactgcccg ccggtacacc aaactgcgct
    49741 gaaattaccg gtagcgcagg cagatacata tcaatcgaca gcggcatcaa catggccagc
    49801 aggccaagga taaaaacaat agcaaacgac gaatgctgtc gggtggtcac aacgggctcc
    49861 tgaaagtcat tgaaaagtta gatgacgctg gcaatttctt cttcagttaa cggacgatat
    49921 tcaccggggg ctaaatcagc atccagcgta atgccgccaa tacgttcacg atgcagctca
    49981 accacatggt tacccacggc ggcgaacatg cgtttcacct gatgataacg cccttcgctg
    50041 atggtcagac gaacctgcgt tggggtaatc acttccagca ccgccggctt agtgagatct
    50101 ttttcgttat gcagctgcac gcctttagca aattgctctg ccgtatcgtc agctacgggt
    50161 gattccagcg tcaccagata ggtcttctcg caatgatggc gcggggaagt aatgcggtgc
    50221 gaccactgac catcatctgt cattagtacc agaccggtgg tatcgatatc cagacgccct
    50281 gcagcgtgca gtttccacgc taccggttca tcaagaaaat agagcaccgt tgggtgatcc
    50341 gggtcgtccg tggagcaaac atagccctga ggcttattga gcatgaagta acgtggaccg
    50401 tgttgctgcg ccagcgggtt gccatcgtaa gcgacatcat gttcagggag cagtttgaac
    50461 gctgcattac ggacgatttc gccatcgacg gtgacacgat tgccgcggat ttcacgcccg
    50521 gcaatagcac ggctaacgcc gagttgctgt gcgataaatt tatcaagtcg catgtgtgtg
    50581 attttgcctg taaaaaacgg aggtcgggta atgacccgaa aatctgaact gttgtctgcc
    50641 cagtatagcg gtctaataac ttccctcaag ggaaaaagat ccgtggcata ctattagcag
    50701 aataatctac ctacgcgaga ccatgatttt tacactccgc ccatatcagc aagaagccgt
    50761 ggatgccacg ctcaaccatt ttcgtcgtca taaaacccct gccgtaatcg tgctgcccac
    50821 cggtgcaggt aaaagcctgg tgatagcgga actggcgcgg ctggcgcgtg gtcgcgtgct
    50881 ggtgctggca cacgttaaag aactggtggc gcaaaaccat gcaaaatatc aggcgctggg
    50941 gctggaagcc gatatttttg ccgccgggct aaagcgcaaa gagagccacg gtaaagtgat
    51001 atttggcagc gtgcagtcgg tcgcccgtaa tcttgatgcc tttcagggtg aattttcgct
    51061 gttgattgtc gatgaatgcc accgtattgg tgacgatgaa gagagccagt atcagcaaat
    51121 cctcactcac ctgaccaagg tgaatcccca cttacgcctg ctggggctga ctgccacgcc
    51181 ttttcgactg ggcaaaggct ggatctacca gttccattat cacggcatgg tacgcggcga
    51241 tgagaaagcc cttttccgtg actgcattta tgagctgccg ctgcgttata tgattaaaca
    51301 cggctatctg acgccgccag aacggctgga tatgccagtc gtgcaatacg atttcagccg
    51361 cttgcaggca cagagtaacg gactgttcag cgaagccgat ctcaaccgcg agctgaaaaa
    51421 acagcaacgc attacgccac acatcatcag ccaaatcatg gagtttgctg caacgcgcaa
    51481 aggggtgatg attttcgccg cgacggtcga acacgcaaaa gagattgtgg gattactgcc
    51541 tgccgaagat gcagcactga ttactggcga cacacccggc tctgagcgcg atgtgttaat
    51601 tgatgatttt aaagcccagc gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg
    51661 atttgacgcc ccgcacgtcg atcttatcgc cattctgcgc cctaccgaat cagtgagtct
    51721 ttaccaacaa attgtcgggc gcggtctgcg cctggccccc ggcaagactg attgcttaat
    51781 tcttgattat gcgggtaacc ctcacgatct ctacgcgccg gaagttggta caccgaaagg
    51841 caaaagtgac aacgttccgg tacaggtttt ctgccctgcc tgcggttttg ccaacacctt
    51901 ttgggggaaa acgaccgccg acgggacatt gattgaacac tttggtcgtc gttgtcaggg
    51961 atggtttgaa gatgacgacg gtcatcgcga acaatgtgac ttccgtttcc gttttaaaaa
    52021 ttgcccgcaa tgtaacgcgg aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac
    52081 cgtactggtt gatccggatg atatgttaaa agcggcgcta cgactgaaag acgcgctggt
    52141 attacgctgt agcggcatgt ctttgcagca tgggcacgac gagaaaggcg aatggttgaa
    52201 aatcacctat tacgatgaag acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc
    52261 cgcccagcgt accgccttcg agcagctttt tatccgcccg catacgcgca caccgggcat
    52321 ccctctgcgc tggatcaccg ccgccgatat cctcgcccag caagcattac tgcgacaccc
    52381 ggattttgtc gtcgcccgca tgaaaggtca gtactggcaa gtgcgtgaaa aagtgttcga
    52441 ttacgaaggt cgttttcgtc gggcgcacga attacgcggt taatggtagt tttgattgat
    52501 gtataaggcg attgagtata gaatctcgcc cgcttttgca tacgcaaagc agatcactta
    52561 cctgttgctg ggtcgcctgt agcaggatta atttaagaga gaaagaaatg tttactatca
    52621 acgcagaagt acgtaaagag cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca
    52681 agttcccggc aatcatctac ggtggcaaag aagcgccgct ggctgtcgaa ctggatcacg
    52741 acaaagtcat gaacatgcaa gttaaagctg aattctacag cgaagttctg accatcgttg
    52801 ttgacggtaa agaaatcaaa gttaaagctc aggacgtaca gcgtcacccg tacaaaccga
    52861 agctgctaca catcgacttc gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc
    52921 tccggcgggg ttttttgtct ctgcaaatta tgcctgatta cggtattgct attttttgca
    52981 ggccagataa gacgttcacg ccgcatctgg cattcagaac acaaaaccga ttaattgccg
    53041 ccagacgtcc ggcgctgcaa ttgatcgcgc aaattcggcg gcgtgccttt aatggtcagg
    53101 gtatcggttg ctggatccca gaagatgcgt tcacctaaca gcatcgcatc aaagttaata
    53161 gtcaagccac cgccgctacc ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc
    53221 gggaagctct cttccagttc gtagcctttt tcagccgcaa actccgtgaa gctcacttcg
    53281 ctgacgcctg ccagctcttt cgacagcgat tccagttcaa tctcttcacc tgcctgcagc
    53341 tgctcattac agtagctata aacctgctgg cgcacattct gtcgctctgc tttatccagt
    53401 tgtgcttcgg cagtgaaatc atcgaccgct tgtaacagcc cacggttttg cgctttcgcg
    53461 ttcaaccctt cgctggcacc gaggaaatcc ataaagaaat ctgccacttt gcgccctact
    53521 cgccctttca ggaaagtgag atagcgggtc gactctggat tggtttccca ttcggttaaa
    53581 tcgatacgcg caacaatatc cgcatgattg atatcaagat aatgagttgg gttgatgtcg
    53641 agattttcgt taacgcgcat actgctcaga ttgctcagaa ctgccaccag caaatactcc
    53701 accgccagat agcgatagtg gcaaaacagc acgaatccac cgtcagcaaa aggatatttc
    53761 gctaattcgt cacgcaggcg accggttgcc gcgcggctaa atgccagaaa gtcctcttcg
    53821 ccctgacgct gtaagcgcag cgtctgcgcc agttcactct cttcgctaaa caagccgtaa
    53881 gctttatttt tggcgctata gacccgatgc agttctgcca ccatctcaac gacggtttcc
    53941 gtcggttcca gcaatgaatc gcgcaacacc agctcaaggt tctgctcatc acgcttgata
    54001 agctggtgca gagcaatctg gttgatatcc agactcatga taaactctcc tttaagaccg
    54061 ggcggtattc aaccaccgcc ggttaagcga cgcaataaaa gatggcggtc attttacaga
    54121 aggcagcgta acgagaatga tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg
    54181 taatatgttg ccctttcatc aactaactga tttcgattta tgccacaaat ttcccgctac
    54241 agtgatgaac aggttgaaca attgctcgca gagctgctca acgtactgga aaaacataag
    54301 gctccgaccg acctttccct gatggtgtta ggcaacatgg tcactaacct tatcaacacc
    54361 agcattgccc cggcccaacg ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc
    54421 tctatcaacg aagacaaagc gcactaaggg aaacagataa caggttatgg taactcatcg
    54481 tcagcgctac cgtgaaaaag tctcccagat ggtcagttgg gggcactggt ttgcactgtt
    54541 caatattctg ctttcgctcg tcattggcag ccgttacctg tttatcgccg actggccgac
    54601 aacgcttgct ggtcgcattt attcctacgt aagcattatc ggtcatttca gcttcctggt
    54661 gttcgccacc tacttgctga tcctcttccc gctaaccttt atcgtcggct cccagaggct
    54721 gatgaggttt ttgtccgtca ttctggcaac ggcgggaatg acgctattac tgatcgacag
    54781 cgaagtcttt actcgtttcc atctccatct taatcccatc gtctggcagc tggttatcaa
    54841 cccagacgaa aatgagatgg cgcgcgactg gcagctaatg ttcatcagtg tgccggttat
    54901 tttattgctt gaactggtgt ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg
    54961 tcgtcggcgc ttcgcgcgcc cgctggccgc attcttattt atcgccttta tcgcctcgca
    55021 tgtggtttat atctgggccg atgccaactt ctatcgcccg atcaccatgc agcgcgctaa
    55081 cctgccgctt tcgtacccga tgacggcacg acgttttctt gagaagcatg gcctgcttga
    55141 tgcgcaggag tatcaacgcc gtcttattga gcaaggcaat ccagacgccg tttccgttca
    55201 gtacccgtta agcgaactgc gctatcgcga tatgggcacc gggcagaatg tgctgttgat
    55261 tactgtcgat ggcctgaact actcacgctt cgagaagcag atgcctgcgc tggcaggttt
    55321 tgctgagcaa aatatttcgt tcacgcgtca tatgagctcc ggcaacacta cagacaacgg
    55381 catctttggc ctgttttatg gcatctcgcc aagctatatg gacggcattt tgtcgacccg
    55441 tacgcctgcg gcgttaatta ctgcgcttaa tcagcaaggc tatcagctgg ggttattctc
    55501 gtcagatggc tttaccagcc cgctgtatcg gcaggcattg ttgtcagatt tctcgatgcc
    55561 gagtgtacgc acccaatccg acgaacagac cgccacgcag tggatcaact ggctgggccg
    55621 ctacgcacaa gaagataacc gctggttctc gtgggtctct ttcaatggca caaacattga
    55681 cgacagcaat cagcaggcat ttgcacggaa atatagccgg gcagcaggca atgtcgatga
    55741 ccagatcaac cgcgtgctca atgcactgcg tgattctggc aaactggaca atacggtggt
    55801 gattatcact gccgggcggg gtattccgct gagcgaagag gaagaaacct ttgactggtc
    55861 ccacggtcat ctgcaggtgc cattagtgat tcactggcca ggcacgccgg cgcagcgtat
    55921 taatgcgctg actgatcata ccgatctgat gacgacgctg atgcaacgcc tgctacatgt
    55981 cagcacacct gccagcgaat attcgcaagg tcaggatttg ttcaaccctc aacgccgcca
    56041 ttactgggtt accgccgcgg ataacgatac gctggcaatt accaccccga aaaagacgct
    56101 ggtgctgaac aataacggta aataccgcac ttacaactta cgtggtgaaa gagtgaaaga
    56161 tgaaaaacca cagttaagtt tgttattgca agtactgaca gacgagaagc gttttatcgc
    56221 taactgatta attataaatc agttagcaaa atatcttact tgcaatcggt gtggaaaacg
    56281 gtagtattag cagccacgag tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt
    56341 gtcgggggtc ggaggttcaa atcctctcgt gccgaccaaa aatcccaaga aaaccaacca
    56401 attgcggttg gtttttttat atctgcattt aattcgataa acagacagcg acacatcacg
    56461 gcctgtttat tttctgttat cagaacgtcc agactacacc cgcctgagtt gtgctgcttt
    56521 cttcacggga gaaacggctg tcggtaccaa gcgttagccc cgtcaatgtt tgctcaaagc
    56581 cagcgcctgc atcagtggtc acgttgttgc ggctgttaat tgccgaactc cacatcgccg
    56641 tatcgtaatt aacgcctttt acgctaccaa gacgcccacg cacggtgtcc agttctgcat
    56701 caaataccag cggttgtgct gccgccatat tcagcacatc cgtggttgaa gggggcattt
    56761 gtgcgcgatt ctctgcgaga ctccagctat ggttgccatt atccagcaag gtatattcat
    56821 acgtactgat atcaacaacg cctccggcat tgcccaacgt aaatgccgca tcaccgcgcc
    56881 cgttgttacc agtgtaaggt tatctcctgc tgccgggctg gcaccggtgt ccgtcacgaa
    56941 tattttgaaa tcacctgttg ctttaacctc cagtagccct cccttttcta taacggcaaa
    57001 gacggcaatc ccatcttttt caacaattaa gcgccctcct ttaatcgtgg gatcagtcgc
    57061 cgcgccataa acctgtagca ctccattatt gatcgtagtc tggtttaccg cgccacccat
    57121 ttgaacaatt tgataaccat catttaatac tgagtattct gcggtgccat ttatttcaat
    57181 gtactgatat cccccgttaa tttcagtatt actacttaca ccaaattctt atatatgctg
    57241 ttctccgcca ggattgattt tcgtattatt ggcggcccta caaaccagct ggatatcttt
    57301 ttcccggaca acaccatcaa ctgtctcacc atactcaaca gttgatgcca atgctatggg
    57361 cgaatataac gctgtagcca ccagaacaga tattgcggaa cgacaaagag aaacagaacc
    57421 agattggtgc attaagcttt catcctatga aattaattgc tgttaaaagc attggggaca
    57481 gaaaataccc atagctccat acccggagtc agttttaaaa aactgtttaa agaaatgcac
    57541 aagtattgtg attgattttt tagttgtttt tcctgatgag aagctgatgc aaaattccgt
    57601 ctttataatg aagatgatgc caaagcgaac gacaaagttg tagttttcac tacatgtcca
    57661 tacataaaat ggggtaacat tcacgcgcct ggtagcgtta ccaacgctac gctcaaacat
    57721 aatgattcta ataaaacctc aggagactac tatgcctgaa gcaacgcctt ttcaggtgat
    57781 gattgtggat gatcatccac ttatgcgacg cggtgttcgt cagttactgg agcttgattc
    57841 tggctttgaa gtggtcgccg aagcgggcga cggcgcaagc gcgatcgatc tggcgaatag
    57901 actggatatc gacgtgatct tgctggatct caatatgaaa ggcatgagtg gcctggatac
    57961 cctcaatgcc ttgcgcaggg atggcgttac cgcgcaaatt attatcctga ccgtatcaga
    58021 tgcctccagc gatgtctttg cactgataga cgcaggcgca gacggttatc tgttgaaaga
    58081 cagcgacccg gaagtgttac tggaagcgat tcgtgccgga gcgaaaggca gcaaagtctt
    58141 tagcgaacgc gtcaatcagt acttacgtga acgtgaaatg tttggcgcgg aagaagaccc
    58201 cttcagcgtg ctgacagaac gcgagctgga tgttctgcac gagctggcac aggggctgtc
    58261 aaataaacag attgcctcgg tgttgaatat ttccgagcag acagtaaaag tccatattcg
    58321 caatctgctg cgtaaactca acgtccgctc acgcgtggcg gccaccattc tgttcctgca
    58381 acaacgcggg gcacaataaa aatagcccga tggcatcacc atcgggccac tttttattta
    58441 ctctcctgcg gcgacaaatg ttgcatcgcc tgcgcgatac tacgttcgat caccgcacgg
    58501 cgagtatcgt tggcaggtaa gagtttcaac atcatttccc acgcggcaac ggcttcgcca
    58561 aatcgttgct gctcaaaggc attaaatgca tacatgctta acacacggat attgctatgg
    58621 tccgttctca ccaactggcg cagcagttcg ccgccgaggc ggttgtcgtt gggatcagat
    58681 gaacgtgtca acgcttcagc gtatcccagt gcagcatcgc tgtttttcgg atcgaggcga
    58741 tacgcagtgg cgtatgcatc ggtggcgata ctggcgttac ccagcgccat gccaacgcgg
    58801 cccaacataa tccagccttc tatatctccc ggattttttt gcagttgagt acgcatcccc
    58861 agcgcaaggc gcgacatctc ttcttcgttg agcggatcgg ctttcggatc cagcgccctg
    58921 tccagtaacg ccggagcctg tgccgtggcc tgctgccaga ttttcacctg ctgataattg
    58981 ccagtctggt agtagctgac gccagccaca attaacgcca ccacaatacc cggcagataa
    59041 acaacatatc cggcacgctt accttccggc acgctttgtt ctggaaacgc ttccggcacc
    59101 acgcgtaccc gacgccgcga acgggcgtat atgacccaac cgccaatgcc aatagccact
    59161 actggcagca cccacagcag cacggtcagc ggcgttaacg gcggatcgta agtgacgaag
    59221 ttgccgtaac gcgccaccat ataatcgaca atctctttct tacttttgcc ttcctgcatc
    59281 agttcataca ctttctggcg caggtcggtg gcaatcatcg agttggaatc ggcaatgctg
    59341 ttgttctggc atttcgggca gcgcagttct tcagtgagct gacggaactg ttgttcctgt
    59401 gcttcatctt taaactgcaa cacgtcgatg gtcgccagcg ctgagccgga gatcatcagc
    59461 atcagcacgc ccaataaaaa cctcattgtg cggcctcctt actgtatttc tcccacagtg
    59521 gcttgatctc ttcttcccag acgcgaggat tgagatcgcc cgcatggcga tagcgaatga
    59581 tgccgttgcc gtcaataagg aacgtttctg gcgcgccata gacaccgaga tccagcccta
    59641 acatgccgtc gccatcaaac aggcttagcg cgtaaggatt gcccagctct ttcagccagc
    59701 tgattgcctt ctggcgatcg tctttatagt tcatgccgac cacgcggatg ccctgcgcag
    59761 aaagctgatt cagatattga tgttccgcac ggcaggtcgg acaccaggtc gcccagacgt
    59821 taagcagcac cggtttgccc tgagtcagca catccgcctg ataaaactgc cccggattgt
    59881 ccagtgattc gaggcgaaac ttcggcactg gcttgccaat gagcgccgat tccagattgg
    59941 tcggatcatc cccttcggca ttacgcgcca gctgccacag cagcgccgcg gcaatcgcca
    60001 ggaagataat caacggaatt aacaatacct tgcgcttcat gcagcctccg gcgcagtttt
    60061 ctgcgggttc acgcgcttac gatagcgagg atcaaacaga cacagcagtc cgcccaacgc
    60121 catcatcagc ccgcccgccc aaatccagcg aacaaatggt ttgtagtaaa gacgcacggc
    60181 ccacgcgccg ttttccagct cttcaccgag tgccgcgtac aggtcacgcg tgatgccacc
    60241 gtcaatcgcc gcttcggtca tcatcgaccc ggcagtgttg taataacgtt tttctgcata
    60301 cagcactgtt tccggcttgc catctcgcgt tacgccgata gtcgccacac cgccacgcca
    60361 gttcggaccg gtcacctctt tgacatcacg gaaggtgaag cgatattcat gaatatcgac
    60421 gctatcgccg gacttcatgc gcacatcacg ctcaacgcta tagttctgac taaaagcaat
    60481 gccaacaatt gtcactgcca gccccaggtg agccgccacc atcccccaat aactgaaggt
    60541 ggttttcgtg ccgcgtgaaa tacgcagcgc agcttccgca attgccagca ccgcaatcca
    60601 gcaggccatt gccaggccga gcactgtcat cgccacaact ttgctttcga acagccacgg
    60661 caacagcagc gacagcacca gcgtagagat gaaggcgata atcaacaaat tgcggatttt
    60721 acgtgggcga tcgcgtcccc agcgcaccag agggccgaca ccaagcagta gcgcaaacgg
    60781 caccatcaac caggtaaaca tggtgttgaa gaacggttcg ccaatcgaaa tactgcccag
    60841 tcccagttgc ttgtgcacca gcggcagcaa cgtccccagc aacaccacca gcatcgcggc
    60901 gaccagcaaa acgttgttcg ctaacagcaa agattcccgc gaccacagcg cattgtttac
    60961 gcgtgagcga actttgtgtc cacgcgcggc aaacagcagc agcgaaccgc caatcaccag
    61021 caccataaag gcgaggataa acataccgcg cgccggatca gacgcaaacg cgtgtaccga
    61081 taccagcacg ccggaacgca ccaggaaagt ccccagtaga cacaacgaga aggcactgat
    61141 tgccagcagt aatgtccacg ctttgaagct ggcgcgttgt tcagtgaccg ccagtgagtg
    61201 catcagcgca gtccccacca gccacggcat aaacgaggcg ttttctaccg gatcccagaa
    61261 ccaccagccg ccccagccga gttcgtaata ggcccatgcg gaaccgagca caatgccgag
    61321 cgtcaggaag atccacgcag ccagcgtcca cggacgagta aaacgcgcat aagtgctgtc
    61381 cagacgccca ctcagcaaag aagcaatggc aaaagcaaac gccaccgaga aacccacgta
    61441 ccccatataa agcagaggcg gatggaagat cagccccgga tcctgcaaca gtgggttaag
    61501 atcacgccct tcaatcggga aattcggcag cgtgcgtgag aacgggttag aggtaaagag
    61561 aatgaacagc aaaaagccga cactgaccat ccccattatc gccagtacgc gggcaacaat
    61621 atccagcgga atacgctgac taaaaatcgc taccgcaaag gtccagccgc tcatcagtag
    61681 cacccacagc aggagcgagc cttcatgcgc gccccaggta gccgccacgc gataccacac
    61741 cggaagctgg gtattggagt tgctggcaac ataggtgacg gtaaagtcgt tgaccacgaa
    61801 agcattgacc agcaccagaa atgcgccagc cacagacata aacagcagcc aggcaaacaa
    61861 gcgggaagac gccatcatgc gcgcatctcc gcgcgctacg ccccatagcg gatacacgga
    61921 cagcagcagc gcaattccta gcgccagaca cagcagcccg ttaccaattt ctggcatcat
    61981 gatgctgggt ccttataaac actcgccggg cgacggtggt tagcttccat cgctttctca
    62041 acttctggcg gcgtgtagtt ttcatcgtgt ttcgccagca cttctttcgc gaggatatga
    62101 ttgccttttt ccagttcgcc ctgcaccaca acgccctgcc cttcacggaa cagatccggc
    62161 aaaatgcctt cgtaagagac atctactgaa ccttcagcgt cgtaaatggt gaaagttact
    62221 tttagcgaat tgggatcgcg ctgcacactc cccggcatca ccatcccgcc aacgcgcaga
    62281 cgctgaccga cttccggcat ctgctgagtt tcacgcttgc cgtagagaat ctcccccggc
    62341 gtataaaaga gatcgatatt cgagcgcagc gcatatagca ccagaccgat agtcagcgcc
    62401 agccccgcca gcacggcaca ggcaatccac aagcggtttt tacggcgaat attcatgcag
    62461 cctcctgctg ttgcgcagca cgtaaacgcg cctcacgcgc ccgctgttgc gccacgccac
    62521 gcagaattgc gcgatgttgc atcaccgagt gcacgaccaa aaccaccagc ggaataacgg
    62581 tcatcaccac cgccagccag acaaaaaagg cgtaaccgcc cattgcgaaa aattcattcc
    62641 aggaagcaaa tgcaggggtc atttacggcc tctttttagt atcagttcac tcacccacgg
    62701 acggcgtttt tccatcagca aaatcaaatt acgcatccgc atcagcgtca gcgtggcaga
    62761 cagcagcagg aaaccaaaaa tcgaccagcg cagcggcgaa cgcatcgccg gatcaatact
    62821 ttgctgcatc cgcgttgatc cctgatgcag ggtgttccac cactccacgg agtaatgaat
    62881 aatcggcagg ttcaccacgc caatcagcac caggatacct gccgcacggc cagccagacg
    62941 acggtcgtcg aaggcgtgcc acagggcaat gacaccaaca tacagaaaca gcagcaccag
    63001 ttcagaagtc agacgtgcat cccataccca ccaggtgccc cacatcggtt ttccccatgc
    63061 agagccggta accagggcaa taaaggtaaa cacggcacca atgggggcca tcgccgccac
    63121 cgccaggttg gccattttca tctgccagac caggccaata aacgctgcca ccgccattga
    63181 tgcataaatg cccatcgacc agatcgccgc aggcacatgc aggtagataa tgcggtagct
    63241 atttccctgc tgataatcag ccggagcaaa gccgaacccc cagatccagc cgacggtaag
    63301 cacgaccaca ctggcaattg ccagccacgg tataaaccag ccacagattt gatacagccg
    63361 tggtgggatc gccagttgat gcagtgtttt ccacatagtt tcgataccag actcgaacaa
    63421 aaatcagtaa tccagcgtta ttgaatgctg attcgtaacg ctgccgccgt cgcaaaagga
    63481 cttaatgtcg cggtgcctgc cagcaacgcg cctaaaattg ccagataccc atcaacgggc
    63541 aaatgcatag aagccgcgtc catcgcggcg gtggcaaaga tgagtaatgg gatagtcagc
    63601 ggtaacacca gtatgctgag cagcacacca ccgcgcttaa gtcccactgt cagcgccacg
    63661 cccggcgcac caagaaagcc aagcgtaggg gttcccagca gtagcgttaa cgccatcact
    63721 tgccagccat aaacatccat tcccagtagc attgctacca ggggcgaaag gatgagtaac
    63781 ggcagaccgg ttaccatcca gtgcgccatc accttcgcca gcacagcggc gggcaagggt
    63841 aacggcaaca acatcaattg ttcaagactg ccgtcctgca aatcgtcacg gaacagtcgc
    63901 tcgagtgcca gcaaggatga aagcagcgcg gcaacccaga taatgcccgg cgcaatacgc
    63961 gccagcagtt gcggctccgg accgatactg agcggaaaaa gggtaattac aatcaggaag
    64021 aaccacagcg ggttggcgat ttcggcgcta tggcgaaacg ctacgcgcag ctcaagacgg
    64081 aaaatgcgcc agaacatcat gcggcccccg tttgcgtcag tgaaatgcgg cgaattttac
    64141 tttcagcaac gttgagcggc tggtgggtag tcagaatcac aatccccccc tgctccgtat
    64201 gctgcgccat acgctgggtc agacgatcga cgccgttaac gtcaatcgcg gtaaaaggct
    64261 cgtcgagtat ccataacgtg gcacgggtca accacagacg cgctaaagcg acgcggcgtt
    64321 gttgcccggc agaaagttga tttaccggaa tatcttcgaa tccggcaagc ccggcctgcg
    64381 ccagggcttc cagacattgt gcggtatcgc catcgcgatg ataaaagtgc agattttcta
    64441 acgccgtcag ccgggttttg atccccggct gatggcctat ccataacagg ttttgatggt
    64501 agctgtcgcg tacctgatgc aagggctgcc cttgccagag aacctcgcct gcatcagggc
    64561 gagacaaccc cgtcagcaaa cggagaagcg ttgtcttccc cgcgccgttg ctaccggtga
    64621 tttgtaccca ctctcctgcg ttcagcgtaa atgataaacc actaaataag gttcgttcat
    64681 cccgctcgca aagtaactct ctggcttcaa gcatacccac gcaataaccc tgttaaaaac
    64741 ctggctcgac ttcacgcatg tcgggcagct tgtgcgctat ccctttatgg caatcaatac
    64801 aggtttgccc atctttcacc gcctggtcat gcatcttcgc ggcaaccgat ttctgggcgg
    64861 ttgtatccat atactcgaag ttgtgacagt tacggcactc ctgcgagttg ttgtccttca
    64921 tgcgccgcca ctcattctgt gccatcgtca ggcgatgagc ttcaaatttc tgcggcgtgt
    64981 caataacgcc aaaaatttta ccatacagct ctttacttgc tttgagcttg cgtatcatct
    65041 ttggcacaaa ctcgtgcgga acgtgacaat ccggacaggt cgcacggacg ccgctacggt
    65101 tgttgtagtg cacggaatcc atgtattcct gatacaccgt gttacgcatt tcgtggcagc
    65161 taatgcagaa ctcttcggta ttggcttttt ccatcccggt gttaaagccg ccccagaaga
    65221 cgatgccgcc gacaaaaccg atcaacagca gcgtccctag cgccagacgg ctgggggtac
    65281 gccaccattt ccacaggcgc ttaatcagac caggcttacg gtcagaattt cccataataa
    65341 cctcttattt cccgtaacct tttgatgggg taaaggtatt ccccacgatt ggcgcggtat
    65401 cggcctgcgg tacgtgacat tgcagacaga aataacgacg cggagccact tccgcgccca
    65461 ctttgccgtc gctgtccata aagtgagtag gactaatgcg cggcgcgcca gtggtgcgat
    65521 aactttcgac accgtggcac tgcaagcagc ggttggtatt ggtcgttacc tgataacctt
    65581 caacgctatg cgggatcatt ggcggctgat tcacatagtt cagcggcatc cgatcctgct
    65641 cttttggcat ccgaatggcc ccttcctgcg tcccggatac ttccggcgac tggctaaaat
    65701 cgacgccatt agccgcccaa accgcgccgc ttaccaccag ggccagcatc gccgtccatt
    65761 gacacagcgc tttcttcagg tcatggcttt tcatgatttc gctcccgaac tccatcgtgt
    65821 agttattgta aatacatcct cagaacagac atccacgcag cgaccgcacg tcatgcaatc
    65881 gcggctggtg acctgcaccg ggctttgttc atccagcacc ggggcacgta gcacatgcgg
    65941 ttccgggcaa acatgaaaac aatccataca gcggttacat ttctgccgat cggtggccgc
    66001 aacggtaatc acacctttgc tacccagcac gccatacagt gcaccaaccg gacaaatgtg
    66061 cccgcaccag ccgtgttcaa cgaccagtag atcaaataaa aacagcgcga gaataagcag
    66121 cgcgccgctg ccgaagccca ttaccaggct acgccccatc agagaaacgg ggttgatcca
    66181 ttcccagatg agcgtgccag ttaaggccga tcccaccaga atgaccacca acagcacgta
    66241 gcgaatatgg cgagggatcg tcgcagactg attgaggtca aatcgtctgc gtaaccaatt
    66301 tgctaagtcg gtaatcgggt tcagcggaca gacccagctg caaaataatc gctttcctgc
    66361 cagggcgtag agcacggtga taatcaccgc accggtcaac gccaccgtgg caggcagatg
    66421 accgctggca agactttgca gtgtcatcag cggatcggtc agtggaacgg tgtcgaataa
    66481 caggctactg ctgtagttgc cgtgcaagat ccacacacca aaccacggac cgctcaaaaa
    66541 catccccagc acgaagaact gacaaaggcg acgtaacact aaccaacggt gactgcgcca
    66601 ccagcctttt ttctccagcg cctcgcgccc ggcgtcacgt ttacgatttg ccattgttcc
    66661 cctccagcca gccgaagcgg taatggtgac ctaactcccc tttcgccagt gacagcggta
    66721 acaccttgat tgccggttgt tccagcacgc acactttttc gcacttaccg cagccggtac
    66781 aggcgtcgct gtgaaccgtc ggcagaaagc gggcgtgctt accggtacgc gtgttgcgct
    66841 ccagctccag ggtgatggcc acatcaattt tcgggcattc gcgataacaa acatcgcagc
    66901 gcagcccctg aaagttgaga cagttttcct ggtccaccag taccgccagc cccatccgcg
    66961 cgtcgtcgat cgattcgatc tcacgatcca gcgcaccgct cgggcacact ttggcgcacg
    67021 gaatgtcctc acacatttcg caaggaatat cccgtgcgac aaaatatggc gtgcccgccg
    67081 acagaccaga ggccagggtc gccagtttta aggtgtcgta agggcaagcc tgaacacact
    67141 gaccgcaacg cacacaggca ctggcaaagg cgttctcgtt tatggctccg ggcgggcgca
    67201 accgcacgcc agatgcgcgt gcggtttgct gttgtaaccc cagcgccaca cccacggcag
    67261 ccagcccgcc tgctgtgcga acgacatcgc gcagaaagcg gcggcgacca ttttgaggtt
    67321 tcgctgaccg ggacataatg gcttacacct tctccagttt gaccgcgcac ttcttgaagt
    67381 ccgtctcttt cgagagcgga tcggtcgcat ccagcgtcag tttgttaacc agctgtgcgg
    67441 cgtcgaagaa cggcatgtac accaggccct gcggtggacg gttacggccg cgcgtttcga
    67501 caatcgagat cacttcgcca cggcgagaaa ccactttcac tttgtcgcca cggcgcagat
    67561 cgcgcgcttt cgcatccaac gggtgaataa acaggaccgc ttccgggaag gcgcggtgca
    67621 gttccggcac acggcgagtc atactgccgg tgtgccagtg ctccaggacg cgtccggtag
    67681 agagccacag gtcgtactct tcatccggtg cttccgccgc cggttcgaac ggcagcgcga
    67741 agatcaccgc tttaccatcc ggtttaccgt agaacttata gccttcgccc gctttcacgt
    67801 acgggtcgtt accttcgctg taacgccact gcgtttcttt accgttcacc accggccagc
    67861 gcagaccgcg cgctttgtgg tagtcatcga acggtgccag atcgtgaccg tgaccgcgac
    67921 caaaccatgc gtactcttcg aacagccctt tttgcagata gaagcccagc tcgcgggatt
    67981 catcgttcag ctgatcttcc gccagttcgg ataccgggaa tttgctcact tccggggtgg
    68041 catacagaac ttcgtacagc gttttgccac gcagttccgg tttcttcgcc agcagctctt
    68101 ccggccatac ttcttcagtt ttaaagcggc gggagaactg gactaactgc cagagatccg
    68161 atttcgcttc gcccggtgcc tgtacctgct gacgccagaa ctgagtacgg cgttcggcgt
    68221 taccgtaagc gccctctttc tctacccaca ttgcggtcgg caggatcaag tcggcggcca
    68281 gcgcactgac tgtcggatac ggatcggaaa cgatgataaa gttgcgcgga tcgcgccagc
    68341 ccggcatacg ctcttcatta atgttcggtc cggcctgcat gttattggta cacatggtcc
    68401 agtaaacatt cagtttgccg tctttcagcg cacggtcttg tgccaccgcg tgcagaccga
    68461 ttttcgccgg aatggtgccg ctcgggatat tccacttctt ctcgcagata tcacggtgtt
    68521 tctcgttagt caccaccatg tccgcaggca gacggtgagc aaaggtgccc acttcacgcg
    68581 cagtaccaca cgcggaaggc tgcccggtca gggagaacgg accgcaaccc ggctgggaaa
    68641 ttttgccggt cagcaggtgc aggttgtaga ccaagttgtt ggcccacaca ccacgggtat
    68701 gctggttgaa gcccatcgtc cagtaggaga tgactttctt gttcggatcg gcatacagct
    68761 gcgccagttg ttctaactgg tctttcggca cgccggtcat ttcggcggtt ttttccagcg
    68821 tatattcagc aacgaaggct ttgtaatctt caaagctcat cggttcggag gcgtcagaac
    68881 ccggattctt cgctgctttt tccagcggat gggtcggacg taaaccgtag ccgatgtccg
    68941 tcgccccttt gcgcaggtta acgtgtttgc tgaagaagtc ctgatttatc gcattgtttt
    69001 gaatgatata gttggcgata tagttcagga tcaccaggtc agattgcggc gtaaagatga
    69061 tgccgttatc cgccagctcg aagctacgat gctggtaggt agaaagcacc gccacggtga
    69121 cgttctggtt agaaagacga cggttagtga tgcgcgacca gaggatcggg tgcatctccg
    69181 ccatgttgga gccccacagc acaaacgcgt cagcctgctc gatgtcgtca tagcagccca
    69241 tcggctcatc cataccaaag gtacgcataa agccaactac tgccgacgcc atacagtgac
    69301 gcgcgttcgg gtcgatgttg ttcgaacgga agcccgcttt gaacaacttg gacgcggcat
    69361 aaccttccca gatagtccac tgaccagaac cgaacatacc gatcgattcc ggcccttttt
    69421 ctttcagggc ggttttgaat ttatcttcca tcacatcgaa ggcctgatcc caggtgattg
    69481 gggtaaattc gccttctttg tcatatttac cgtttttcat acgcagcagc ggctgcgtca
    69541 aacggtcttt accgtacatg attttgggca ggaaatagcc cttaatgcag ttcaggccac
    69601 ggttaaccgg tgcgtccggg tcgccctgac aggccaccac acgcccctgc tgcgttccga
    69661 ccagaacgcc gcaacccgta ccgcagaaac ggcacggcgc tttatcccat ttaatggctt
    69721 cctgctgacc aacaacggcg cgggcaacgc ccggcacgct gagaccggca gccgccgcag
    69781 cggccgcaac ggcgttagct ttcataaagc tacgacgact gagtttcatg gtgtttcctc
    69841 accttgctct tcctgctggt gataaaccag cgacaccgcc agcacgccct ctacgttgcg
    69901 tactgactca atggtttgga tcagcgtttc gctgtcttct gcttccacca ccacaatcaa
    69961 ctgaccgctc ggcgcgtcgc tgacagcaac ttcacagccg gtaaaggcgt tcagttgggt
    70021 gctgatgtct gaaattcgtt cgcttttggc ctgcacgacc aggctgcaaa cttgccagtt
    70081 agtgtgcatg gagatactcc gcagttatgg ctgataccgg acagttggct gcacatgctc
    70141 cacatccgtt gcaaagttgg ctattaagtt gcggctggta gatcccggag agcgtcgggc
    70201 gaaagataat tgccattggc tcacagctat cctgacagcg gcggcattca actgactgat
    70261 acgccaggca cgcgtcccca atggtgaact gcaaatccca ggccctggtg tggcgcggag
    70321 aaaatagcga ttcggggcaa gcctgcgcgc aggcgtaaca gaagctgcac tcgttatttt
    70381 tgaaattaac gctcggatag ccacccgcgc cgcgttgcag aatgttgttt tcacaagcat
    70441 taatacaggc gtcgcagcgg gtgcaatggg tcagaaaatg agattcatca ccggaccagg
    70501 gcggacggat accgttactg gctttgcgcc agcgaccagt gagtatgccc cgacgggatg
    70561 catcaatctt cacattgacc ttccatcatt aacgcgctct gaaaattgag agcgaccaaa
    70621 taaaccgcat aattaataag ccatttttat agccgctaag atattaaagg atgtgtcaaa
    70681 gatgcatacc ccgatcgggg taaaacctgt agcaggatca aaagagtggc gggaagcgtg
    70741 gcaaaaacgg gcttttgctc acatttcaaa tggttataaa catatttata tagcgattaa
    70801 ttcgccagag atatttctgc tggtttgttt tctcatcag
//
LOCUS       DAZGJT010000008        68322 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZGJT010000008 DAZGJT010000000
VERSION     DAZGJT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 68322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 68322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..68322
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.008"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..369
                     /locus_tag="ACHKFK_000585"
     CDS             <1..369
                     /locus_tag="ACHKFK_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051002.1"
                     /translation="GTTTGTGTTTGTGTTTGTTTADGADIKVDFDSAPSVSLPDINSP
                     PSGTQIITPLWDMWPEARDFSVSIPAGTCPVFTFHIWDQDYTMDQFCTLLDNDSVRNT
                     FRVVMTLVSSLMAFFIVLKA"
     gene            390..677
                     /locus_tag="ACHKFK_000586"
     CDS             390..677
                     /locus_tag="ACHKFK_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 family protein"
                     /protein_id="HFO9051003.1"
                     /translation="MWAILLAAVNTAIAFIFRAVVIKFVLFTAVYLFISEVVPLIISH
                     LPSAGNISELFSRLPSGVLWLLNVFSVDIFLPVIVSAMFTRFFIRRIPFLN"
     gene            695..1807
                     /locus_tag="ACHKFK_000587"
     CDS             695..1807
                     /locus_tag="ACHKFK_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO9051004.1"
                     /translation="MSIFAYVGVPGSGKSYEVVSNVIVPAICEGRRVVTNIYGINYDE
                     ILDYAEKKKLLKDGIKPGEIICVDNERIIAPDFYPVHENQERSLCKPGDLIIVDECHR
                     FYENDKSLSKDAKIFAAEHRHYADPETGRTCDFILVNQSVTNLPRFFRDRIEKTFRMR
                     KLRQLGLSRSYCVDVYDGAKITKSRFLSNYVCKYSKDVFRLYSSHHVQNAQESTVDSR
                     GVLFKKRTIIFVLIFLSGTVWAVFHYVVAFFNPDENTLVNDVDSRTESQPADNTNLSL
                     NHAVNKPGVGTANLQTTPSDKWCIVGRFSDGRKNWIFVRDAENRLRMASASGFTGYNI
                     MAEGELDGEKITAWSCNKPVSVRSVSSSTLNTGAGG"
     gene            1810..3081
                     /locus_tag="ACHKFK_000588"
     CDS             1810..3081
                     /locus_tag="ACHKFK_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspD"
                     /protein_id="HFO9051005.1"
                     /translation="MKFRILILISFLTFIFPLTSFSAVKQKTSEQISSRVDMSMDNAP
                     LPQVISLIWQRVFNRPFQLSPELAGDTRLVSFYLTQSLEPRSFFISYLKNMGIAVSSR
                     NGVDWIYIPAKKEFKEPVSVFTYRPKYRSVSYLSSMLTSVTQSGSFSNHVQTVDYSGA
                     SASAASSGSAINSVSAATDAEVLVYSGTAADIRRVRDILPRIDIPAEQVTVSGYVLEV
                     QTTERNASGLQIIADLFKNRLGVSLGARLDDGNAFTLNVGGLNAFYSLIKTDSRFNIV
                     SNPRLTVLSGSQSQFTVGQEVPVLDSVTYQGNSGTPVQSVTYRNSGAIFTVTPVVLDG
                     VITLDISQQLSDFVKTTTGVNTSPTLTKREISTRVDVHDGDVLVLGGLASSKTTKARS
                     GFSFLPGFTGSSEDSDKTDIIVVLQADRVSR"
     gene            complement(3407..3700)
                     /locus_tag="ACHKFK_000589"
     CDS             complement(3407..3700)
                     /locus_tag="ACHKFK_000589"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051006.1"
                     /translation="MSSSAQWNIFAGYHTDILSVTINHDLIQQYQPLQNIPNEDKNNK
                     KPIKVWVLMTKFEKWEEQKCRILYQSSGVTEPRTASRLFSNQRITADGESLSL"
     gene            complement(3704..3926)
                     /locus_tag="ACHKFK_000590"
                     /pseudo
     CDS             complement(3704..3926)
                     /locus_tag="ACHKFK_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3992..5737)
                     /locus_tag="ACHKFK_000591"
     CDS             complement(3992..5737)
                     /locus_tag="ACHKFK_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9051007.1"
                     /translation="MTPGEKRVASRLESFLNDDCLVWYDIPVGRKNRHPDFVIIDPEN
                     GLIFLEVKDWTISTLCKANQEQVTLETDGIQKSEINPLVQVRRYACDTVNALPTDPCL
                     RQNDGQYKGRLNLAWAYGVVFTRITRQQLKTLAGNNENAVEKIFPAAQTICQDEMTQS
                     VLPQVFRQKIAGMFTTGFRTRVPPRMRDILRAHLFPEVTVKQNSQTKIMDIQQEILAR
                     NIGDGHRVIHGVAGSGKTMILLFRCLYLAETTPGKILVLCYNITLASYLRECIEAKGL
                     TSRVTVSHFHSWCTSMIKRHGIQVTTDGKNYPEKCFSALEEAVNRGKITDTGYDAVLV
                     DEGHDFDSRWLALIARLFDNSSRSLLLMYDDAQSIYRRERALNFSLASVGIQAQGRTS
                     VLPVNYRNPKCILHFSYAFSREYFEKHQNREIPLLQPQAGGEEGTEPKILRCTSEHDE
                     ARQVADWLEKRHALCGHWSDMAVLCPAEFSVKQLKDIMTQRGIPFTTCFDSEGKKTYS
                     RRRDVVHLLTYQSSKGLEFPYVAVINASFVHSGALDESEVIPVLYVAFTRATRELLVT
                     CYRENSISRHLEDFA"
     gene            complement(6029..7900)
                     /gene="ppiD"
                     /locus_tag="ACHKFK_000592"
     CDS             complement(6029..7900)
                     /gene="ppiD"
                     /locus_tag="ACHKFK_000592"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO9051008.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQSKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(8092..8382)
                     /gene="hupB"
                     /locus_tag="ACHKFK_000593"
     CDS             complement(8092..8382)
                     /gene="hupB"
                     /locus_tag="ACHKFK_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO9051009.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(8573..10927)
                     /gene="lon"
                     /locus_tag="ACHKFK_000594"
     CDS             complement(8573..10927)
                     /gene="lon"
                     /locus_tag="ACHKFK_000594"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO9051010.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(11115..12389)
                     /gene="clpX"
                     /locus_tag="ACHKFK_000595"
     CDS             complement(11115..12389)
                     /gene="clpX"
                     /locus_tag="ACHKFK_000595"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO9051011.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(12515..13138)
                     /gene="clpP"
                     /locus_tag="ACHKFK_000596"
     CDS             complement(12515..13138)
                     /gene="clpP"
                     /locus_tag="ACHKFK_000596"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO9051012.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(13384..14682)
                     /gene="tig"
                     /locus_tag="ACHKFK_000597"
     CDS             complement(13384..14682)
                     /gene="tig"
                     /locus_tag="ACHKFK_000597"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO9051013.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(15026..15343)
                     /gene="bolA"
                     /locus_tag="ACHKFK_000598"
     CDS             complement(15026..15343)
                     /gene="bolA"
                     /locus_tag="ACHKFK_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO9051014.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            15648..16226
                     /gene="yajG"
                     /locus_tag="ACHKFK_000599"
     CDS             15648..16226
                     /gene="yajG"
                     /locus_tag="ACHKFK_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9051015.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            16270..17745
                     /gene="ampG"
                     /locus_tag="ACHKFK_000600"
     CDS             16270..17745
                     /gene="ampG"
                     /locus_tag="ACHKFK_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO9051016.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFFWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNGNFISRTEYPAGYAFAMWTLAAGISLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            18205..19152
                     /gene="cyoA"
                     /locus_tag="ACHKFK_000601"
     CDS             18205..19152
                     /gene="cyoA"
                     /locus_tag="ACHKFK_000601"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO9051017.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAAFDQWVAKAKQSPNSMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            19174..21165
                     /gene="cyoB"
                     /locus_tag="ACHKFK_000602"
     CDS             19174..21165
                     /gene="cyoB"
                     /locus_tag="ACHKFK_000602"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO9051018.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            21155..21769
                     /gene="cyoC"
                     /locus_tag="ACHKFK_000603"
     CDS             21155..21769
                     /gene="cyoC"
                     /locus_tag="ACHKFK_000603"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO9051019.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            21769..22098
                     /gene="cyoD"
                     /locus_tag="ACHKFK_000604"
     CDS             21769..22098
                     /gene="cyoD"
                     /locus_tag="ACHKFK_000604"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO9051020.1"
                     /translation="MSHSNESSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQILVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            22110..23000
                     /gene="cyoE"
                     /locus_tag="ACHKFK_000605"
     CDS             22110..23000
                     /gene="cyoE"
                     /locus_tag="ACHKFK_000605"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO9051021.1"
                     /translation="MIFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            23149..24513
                     /gene="yajR"
                     /locus_tag="ACHKFK_000606"
     CDS             23149..24513
                     /gene="yajR"
                     /locus_tag="ACHKFK_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051022.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQMADAGLPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(24641..25132)
                     /gene="yajQ"
                     /locus_tag="ACHKFK_000607"
     CDS             complement(24641..25132)
                     /gene="yajQ"
                     /locus_tag="ACHKFK_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO9051023.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIAHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            25300..26211
                     /gene="panE"
                     /locus_tag="ACHKFK_000608"
     CDS             25300..26211
                     /gene="panE"
                     /locus_tag="ACHKFK_000608"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO9051024.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHDVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAILNCPNGELRHHQQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            26174..26764
                     /gene="yajL"
                     /locus_tag="ACHKFK_000609"
     CDS             26174..26764
                     /gene="yajL"
                     /locus_tag="ACHKFK_000609"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO9051025.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(26818..28266)
                     /gene="thiI"
                     /locus_tag="ACHKFK_000610"
     CDS             complement(26818..28266)
                     /gene="thiI"
                     /locus_tag="ACHKFK_000610"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO9051026.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQSK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            28472..28714
                     /gene="xseB"
                     /locus_tag="ACHKFK_000611"
     CDS             28472..28714
                     /gene="xseB"
                     /locus_tag="ACHKFK_000611"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO9051027.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            28714..29613
                     /gene="ispA"
                     /locus_tag="ACHKFK_000612"
     CDS             28714..29613
                     /gene="ispA"
                     /locus_tag="ACHKFK_000612"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO9051028.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHIPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LTEQSLDTSALEALADYIIQRNK"
     gene            29638..31500
                     /gene="dxs"
                     /locus_tag="ACHKFK_000613"
     CDS             29638..31500
                     /gene="dxs"
                     /locus_tag="ACHKFK_000613"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO9051029.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELSLDATGMEAKIKAWLA"
     gene            31555..32529
                     /gene="yajO"
                     /locus_tag="ACHKFK_000614"
     CDS             31555..32529
                     /gene="yajO"
                     /locus_tag="ACHKFK_000614"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO9051030.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYQESNENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVTGFK"
     gene            complement(32583..33101)
                     /gene="pgpA"
                     /locus_tag="ACHKFK_000615"
     CDS             complement(32583..33101)
                     /gene="pgpA"
                     /locus_tag="ACHKFK_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO9051031.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVTAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(33079..34056)
                     /gene="thiL"
                     /locus_tag="ACHKFK_000616"
     CDS             complement(33079..34056)
                     /gene="thiL"
                     /locus_tag="ACHKFK_000616"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO9051032.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            complement(34134..34553)
                     /gene="nusB"
                     /locus_tag="ACHKFK_000617"
     CDS             complement(34134..34553)
                     /gene="nusB"
                     /locus_tag="ACHKFK_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO9051033.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(34573..35043)
                     /gene="ribE"
                     /locus_tag="ACHKFK_000618"
     CDS             complement(34573..35043)
                     /gene="ribE"
                     /locus_tag="ACHKFK_000618"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO9051034.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(35132..36235)
                     /gene="ribD"
                     /locus_tag="ACHKFK_000619"
     CDS             complement(35132..36235)
                     /gene="ribD"
                     /locus_tag="ACHKFK_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO9051035.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQGL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGNDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(36239..36688)
                     /gene="nrdR"
                     /locus_tag="ACHKFK_000620"
     CDS             complement(36239..36688)
                     /gene="nrdR"
                     /locus_tag="ACHKFK_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO9051036.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            36839..37378
                     /gene="yajI"
                     /locus_tag="ACHKFK_000621"
     CDS             36839..37378
                     /gene="yajI"
                     /locus_tag="ACHKFK_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO9051037.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNKLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     MSVDQLGFVRIHDIQPVMQ"
     gene            37677..38561
                     /gene="tsx"
                     /locus_tag="ACHKFK_000622"
     CDS             37677..38561
                     /gene="tsx"
                     /locus_tag="ACHKFK_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9051038.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQLRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKVKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGYANNGIKTRTNNSIASSHILALNYDHWHYSVVARYWHNGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(38599..38946)
                     /gene="yajD"
                     /locus_tag="ACHKFK_000623"
     CDS             complement(38599..38946)
                     /gene="yajD"
                     /locus_tag="ACHKFK_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO9051039.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(39076..40047)
                     /gene="secF"
                     /locus_tag="ACHKFK_000624"
     CDS             complement(39076..40047)
                     /gene="secF"
                     /locus_tag="ACHKFK_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO9051040.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(40058..41905)
                     /gene="secD"
                     /locus_tag="ACHKFK_000625"
     CDS             complement(40058..41905)
                     /gene="secD"
                     /locus_tag="ACHKFK_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO9051041.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPSEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDKGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(41933..42265)
                     /gene="yajC"
                     /locus_tag="ACHKFK_000626"
     CDS             complement(41933..42265)
                     /gene="yajC"
                     /locus_tag="ACHKFK_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO9051042.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(42288..43415)
                     /gene="tgt"
                     /locus_tag="ACHKFK_000627"
     CDS             complement(42288..43415)
                     /gene="tgt"
                     /locus_tag="ACHKFK_000627"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO9051043.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(43470..44540)
                     /gene="queA"
                     /locus_tag="ACHKFK_000628"
     CDS             complement(43470..44540)
                     /gene="queA"
                     /locus_tag="ACHKFK_000628"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO9051044.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDQRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            44633..45214
                     /gene="acpH"
                     /locus_tag="ACHKFK_000629"
     CDS             44633..45214
                     /gene="acpH"
                     /locus_tag="ACHKFK_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO9051045.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRNETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFT
                     CYAREQVMTILPDSPPRFIHLNNYLWSEQWLVRYRDMDFIQSVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMEQASRKAL"
     gene            complement(45219..47036)
                     /gene="malZ"
                     /locus_tag="ACHKFK_000630"
     CDS             complement(45219..47036)
                     /gene="malZ"
                     /locus_tag="ACHKFK_000630"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO9051046.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTSEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQLGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAIDVPDIGPQWAADQIFYQIFPDRFARNLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGIRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNLQHVTGITEAAKETQPDAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDVARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     SALFALYQRMIALRKKGQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNVAQWQRKEGHGQLTDGILALPAISATVWMN"
     gene            complement(47192..48565)
                     /gene="proY"
                     /locus_tag="ACHKFK_000631"
     CDS             complement(47192..48565)
                     /gene="proY"
                     /locus_tag="ACHKFK_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO9051047.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(48641..49960)
                     /gene="brnQ"
                     /locus_tag="ACHKFK_000632"
     CDS             complement(48641..49960)
                     /gene="brnQ"
                     /locus_tag="ACHKFK_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO9051048.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIIWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(50367..51662)
                     /gene="phoR"
                     /locus_tag="ACHKFK_000633"
     CDS             complement(50367..51662)
                     /gene="phoR"
                     /locus_tag="ACHKFK_000633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO9051049.1"
                     /translation="MLERLSWKRLVLELLLCCLLAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(51720..52409)
                     /gene="phoB"
                     /locus_tag="ACHKFK_000634"
     CDS             complement(51720..52409)
                     /gene="phoB"
                     /locus_tag="ACHKFK_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO9051050.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            52599..53801
                     /gene="sbcD"
                     /locus_tag="ACHKFK_000635"
     CDS             52599..53801
                     /gene="sbcD"
                     /locus_tag="ACHKFK_000635"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO9051051.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQAHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASVGHTPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDISQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFATTLH
                     SLAGEHEA"
     gene            53798..56941
                     /gene="sbcC"
                     /locus_tag="ACHKFK_000636"
     CDS             53798..56941
                     /gene="sbcC"
                     /locus_tag="ACHKFK_000636"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO9051052.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRLDELQQEGSRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEYSTALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNAWLQEHDRFRQWNNELAGWRAQFSQQTCDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTLEQTQRNAALNEMRHRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSSSHPAVEAYQALEPGVNQARLLTLEKEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLNQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRMQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFYTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLTQHQQHRPGGLSLTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESAFAVK"
     gene            57067..58251
                     /gene="araJ"
                     /locus_tag="ACHKFK_000637"
     CDS             57067..58251
                     /gene="araJ"
                     /locus_tag="ACHKFK_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO9051053.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFFGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSSVLAKP
                     LG"
     gene            complement(58520..59428)
                     /gene="mak"
                     /locus_tag="ACHKFK_000638"
     CDS             complement(58520..59428)
                     /gene="mak"
                     /locus_tag="ACHKFK_000638"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO9051054.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVPQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            59553..60464
                     /gene="rdgC"
                     /locus_tag="ACHKFK_000639"
     CDS             59553..60464
                     /gene="rdgC"
                     /locus_tag="ACHKFK_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO9051055.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSTAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(60545..60625)
                     /locus_tag="ACHKFK_000640"
     CDS             complement(60545..60625)
                     /locus_tag="ACHKFK_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051056.1"
                     /translation="MTQRPWSKLQHKTHNIAALRIIARCS"
     gene            complement(60622..60963)
                     /locus_tag="ACHKFK_000641"
                     /pseudo
     CDS             complement(60622..60963)
                     /locus_tag="ACHKFK_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(61111..61395)
                     /gene="ppnP"
                     /locus_tag="ACHKFK_000642"
     CDS             complement(61111..61395)
                     /gene="ppnP"
                     /locus_tag="ACHKFK_000642"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO9051057.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(61467..62144)
                     /gene="aroM"
                     /locus_tag="ACHKFK_000643"
     CDS             complement(61467..62144)
                     /gene="aroM"
                     /locus_tag="ACHKFK_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO9051058.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNHLAHVSRRKVERDLQGVVEVLDNQGYDVIILMSTANI
                     SSMTARNTIFLEPSRILPPLVFSIVEDHQVGVIVPVEELLTVQAQKWQILQKPPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFNQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLM"
     gene            complement(62402..62593)
                     /gene="yaiA"
                     /locus_tag="ACHKFK_000644"
     CDS             complement(62402..62593)
                     /gene="yaiA"
                     /locus_tag="ACHKFK_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO9051059.1"
                     /translation="MPTKPPYPREAYIVTIDKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(62643..63167)
                     /gene="aroL"
                     /locus_tag="ACHKFK_000645"
     CDS             complement(62643..63167)
                     /gene="aroL"
                     /locus_tag="ACHKFK_000645"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO9051060.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDLWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(63350..63808)
                     /gene="yaiI"
                     /locus_tag="ACHKFK_000646"
     CDS             complement(63350..63808)
                     /gene="yaiI"
                     /locus_tag="ACHKFK_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO9051061.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            63928..64737
                     /gene="proC"
                     /locus_tag="ACHKFK_000647"
     CDS             63928..64737
                     /gene="proC"
                     /locus_tag="ACHKFK_000647"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO9051062.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVTA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGITL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGTLKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(64754..65869)
                     /gene="adrA"
                     /locus_tag="ACHKFK_000648"
                     /gene_synonym="dgcC"
     CDS             complement(64754..65869)
                     /gene="adrA"
                     /locus_tag="ACHKFK_000648"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO9051063.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPSPGWWWLLLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVIYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVHEGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(65971..66291)
                     /gene="psiF"
                     /locus_tag="ACHKFK_000649"
     CDS             complement(65971..66291)
                     /gene="psiF"
                     /locus_tag="ACHKFK_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO9051064.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSVCL
                     KKAA"
     gene            complement(66410..67825)
                     /gene="phoA"
                     /locus_tag="ACHKFK_000650"
     CDS             complement(66410..67825)
                     /gene="phoA"
                     /locus_tag="ACHKFK_000650"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO9051065.1"
                     /translation="MKQSTIALALLLLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWQGPKAAYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(67926..68186)
                     /gene="iraP"
                     /locus_tag="ACHKFK_000651"
     CDS             complement(67926..68186)
                     /gene="iraP"
                     /locus_tag="ACHKFK_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO9051066.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLRHPRQ"
BASE COUNT        16409 a        17994 c        16954 g        16965 t
ORIGIN      
        1 ggaacaacca ccggcacggg aacaaccacc ggaacaggga caaccaccgg aacaacaacg
       61 gcagacgggg cagatattaa ggttgatttt gattctgctc cgtcggtttc tctgccggat
      121 ataaattctc cgccttcagg tacacagata attacgccgc tctgggatat gtggcctgaa
      181 gcacgtgatt tttcggtatc cataccagcc ggaacctgcc cggtattcac ttttcatatc
      241 tgggaccagg attacaccat ggatcagttc tgcacgctgc ttgataacga ttcggtgcgt
      301 aataccttcc gtgtggtgat gacgctggtt tcatccctta tggctttttt tattgttctt
      361 aaagcctgac cgggcaataa ggagttatta tgtgggcgat attacttgct gcggtgaata
      421 ccgcaattgc gtttattttt cgtgccgttg tgattaagtt tgtgcttttt acggcggttt
      481 atctttttat ttctgaagtt gtgccgctga ttatttctca tttaccttca gcgggtaata
      541 tttcggaact gttctctcgt ctgccttccg gagtgctctg gttactgaat gttttttctg
      601 ttgatatttt tcttcctgtt attgtttctg cgatgttcac gcgtttcttt attcgtcgca
      661 ttccttttct taactgacag gagaggaaca gattatgtcc atttttgctt atgtgggtgt
      721 gcctggttcc ggtaaatcct atgaggttgt cagtaatgtg attgttcctg ccatctgtga
      781 aggtcgtcgt gttgtcacca atatttacgg gattaattat gatgaaatac ttgattatgc
      841 ggaaaagaaa aaactgctta aggatggtat taaaccgggt gaaattatct gcgtggataa
      901 tgaacgaatt attgcccctg atttttaccc tgtccatgag aatcaggaac gctcactgtg
      961 caaaccgggc gatttaatta ttgtcgatga atgccaccgt ttttatgaga atgataaaag
     1021 tctttcaaag gatgcaaaaa tctttgcggc ggaacaccgg cattatgcag accctgaaac
     1081 aggacgcact tgtgacttca ttctggttaa tcagtctgta accaatctcc cgcgtttttt
     1141 tcgtgatcgt attgaaaaga cattcagaat gcgaaaactc aggcaattgg ggctttcgcg
     1201 cagttattgt gttgatgttt atgatggtgc gaagataacc aaatccaggt ttctgtcaaa
     1261 ttatgtctgt aaatacagta aggatgtttt cagactttac agttcccacc atgtacagaa
     1321 cgcgcaggaa tctacagttg attcacgtgg tgtgcttttt aaaaaaagaa caataatttt
     1381 tgttctgatt tttctgtcgg gtacggtctg ggctgtattt cattatgtgg tggcgttttt
     1441 taatcctgat gaaaatacgc tggttaatga cgttgattcc agaactgaat cacagcctgc
     1501 ggataacact aatctgtcat taaatcatgc agttaataag ccgggggtag gtacggctaa
     1561 tttacagaca acaccttctg ataaatggtg tattgtcgga cgtttttctg atggccggaa
     1621 aaactggatt tttgttcgtg atgcggaaaa ccgtcttcgc atggcatcag ccagcggttt
     1681 tacggggtat aacatcatgg cagaagggga acttgacgga gaaaaaatca cagcatggtc
     1741 atgtaataaa cctgtttccg tccgttcggt ttcttcgtcg acactgaata caggggcagg
     1801 gggttaataa tgaaatttcg tatactgata ttgatttctt tcctgacatt tatttttcct
     1861 ttgacttcct tttctgctgt taaacagaaa acgtcggagc agatttcttc tcgtgtcgat
     1921 atgtcaatgg ataacgcgcc acttccgcag gtgatatcgt taatctggca gcgtgttttt
     1981 aaccgcccgt ttcagttgtc gccggaactg gctggcgaca cgcggcttgt gagtttttat
     2041 cttacgcagt cactggaacc ccgttcattt tttatttcct accttaaaaa tatggggata
     2101 gcggtttcat ccagaaacgg ggttgactgg atttatattc cggctaaaaa ggaatttaag
     2161 gagcctgtca gcgtgtttac gtatcgcccg aaataccgca gtgtcagcta tctcagttcc
     2221 atgctgacct ctgtcacgca atcaggttct ttctcaaatc atgtacagac cgtagattat
     2281 tctggcgctt ctgcatccgc agcttcttcc ggttcagcaa taaacagtgt ttcggctgcc
     2341 acggatgcgg aagtccttgt atacagtgga acggcggcag atattcgccg tgttcgtgac
     2401 atactgccac ggatagatat cccggctgag caggtgactg tcagcggtta cgttctggag
     2461 gtgcagacca ctgaacggaa cgccagcgga ttacagatta tcgctgatct gtttaaaaat
     2521 cgtcttggcg tttcgctggg tgctcgtctt gatgatggta atgcgttcac tctgaatgtg
     2581 ggggggctga atgcgtttta cagtctgatc aaaacggatt cgcggtttaa tattgtgagt
     2641 aatcccaggc tgacggtgct ttccggcagt cagtcgcagt ttacggtggg tcaggaagtg
     2701 cctgttctgg acagcgtcac ttatcagggc aacagcggaa cgccggttca gtcggttacg
     2761 tacagaaaca gcggggcaat ttttacagtg acgccggttg ttctcgacgg tgtgatcaca
     2821 ctcgatatca gtcagcaact gagtgatttt gtaaagacaa ccacgggtgt taacaccagt
     2881 ccgacactga caaaacgtga aatcagcaca cgggttgatg tgcatgatgg tgatgttctg
     2941 gttcttggcg gactggccag cagtaagacg acaaaggcgc gcagtggatt ttcgtttctg
     3001 ccgggattta caggcagctc tgaggacagc gacaagacgg atattatcgt tgttttgcag
     3061 gctgaccgtg tatcgcgctg aaccggatga ccggttatct ttatgtccgc caggacatgg
     3121 aaggccgcgc gcagtggttt catgtgacgc agttactgaa cggccggagc cagggagagc
     3181 gtgtatattc cgcgggacag cgcaacgcgc ctcctgcaaa gccgggtttt catggcaccc
     3241 gtcgagatga acgacgcaag gcgtgagtcg tccatgctat gatgtggcat gttgatgatt
     3301 tttctgtgac cccaacacgc cgggagggag taggacggag gaacgccagc cgcacccgcc
     3361 cgcaggtacg aggacgggag cgcctgccgt gacgacggac gacggctcac aatgataatg
     3421 attcaccgtc tgctgttatg cgctgattag aaaataaccg tgaagcggtt ctgggttctg
     3481 taacacccga actctggtac aagatcctgc acttttgctc ttcccatttt tcaaatttag
     3541 tcattaaaac ccatactttt attggttttt tattgttttt gtcttcatta gggatatttt
     3601 gtagtggttg atattgctgt attaaatcat gattaatcgt tacggaaaga atgtcagtat
     3661 gatagccggc aaagatattc cactgcgcgg atgatgacac atctcactgc cgtttcagaa
     3721 ccggtaacag attattaatt tgtctggtat aacgttcagt gaataggtgt tgtcctttct
     3781 tgcttaaatg aacaccggat tgtacaggta tcattgctga tatatacagt ttcctgagat
     3841 ctggaactga tattccagcg ccaggaatat ggagaatact tcaggtgaaa aagacacatc
     3901 tgaatatcgt taaataacgg gttcatggcg aattaaataa acctgataaa gacagtatgg
     3961 tgaaagctcg gaataaagaa atagtcaggt attatgcaaa atcctcaagg tggcggctga
     4021 tactgttttc acggtaacag gtcaccagta attcgcgggt tgcccgggtg aacgctacat
     4081 aaagcaccgg tattacttcg gattcatcca acgcaccaga atgaacgaaa gacgcattga
     4141 tgaccgctac atacgggaac tccagcccct tactgctctg gtacgtcagt aaatgcacaa
     4201 catccctgcg tcgcgagtat gtcttcttac cttcactatc aaagcaggtg gtaaaaggta
     4261 ttccgcgttg tgtcataatg tctttcagtt gtttcactga aaactctgcc ggacacagaa
     4321 cagccatatc actccagtga ccgcacaggg catgacgctt ctccagccag tctgcaacct
     4381 gtctggcttc atcatgttca gaagtacaac gcaaaatttt cggctctgtg ccctcttcac
     4441 cgcccgcctg tggctgaagg agcggtattt cacggttttg atgtttttca aaatactcgc
     4501 gggagaatgc ataagaaaag tgcagaatgc atttcggatt acgataattg actggcagga
     4561 ctgaagtacg tccttgagct tggattccca cactggccag tgaaaagttc agtgcccgct
     4621 cacgccggta aatagactgg gcgtcatcat acatcaggag taatgaacgg gatgaattat
     4681 caaatagacg ggcaattaga gccagccagc gactgtcaaa atcatgcccc tcatccacca
     4741 gtacagcatc atatccggtg tcggtgattt ttcctctgtt cacagcctct tccagtgcgc
     4801 tgaagcattt ctccgggtaa ttctttccgt cagtcgtcac ctgaataccg tggcgtttta
     4861 tcatggatgt acaccagctg tgaaaatgag ataccgtaac ccgtgacgtc agccctttag
     4921 cttcaatgca ttctcgtaga tagctggcta gggtaatgtt gtaacacagc accagaatct
     4981 ttccgggagt ggtttcagcc aagtaaaggc aacggaacaa aagaatcatg gtttttccgg
     5041 aaccggccac tccatgaatg acacgatgcc cgtcaccaat atttctggcc agaatctcct
     5101 gttgaatatc catgattttt gtttgtgagt tctgttttac ggtcacttca ggaaaaagat
     5161 gggcacgcag aatatctctc attcgggggg gcacccgggt tctgaatcct gtggtaaaca
     5221 tcccggcaat tttttgccgg aatacctgag gtaaaacaga ctgcgtcatt tcatcctgac
     5281 agattgtctg tgctgcaggg aaaatctttt ccactgcatt ctcattattt cctgccagtg
     5341 tcttcagttg ctgacgggta atacgagtaa acaccacacc atacgcccaa gctaggttca
     5401 gccgtccctt atactggccg tcattctgcc gtaagcatgg gtctgtaggt aaggcattaa
     5461 cagtatcaca agcataacga cgaacctgta ccagcggatt tatttctgat ttctgaatac
     5521 cgtcggtttc cagcgtaacc tgttcttggt ttgctttaca cagtgtggaa atcgtccagt
     5581 ctttgacctc cagaaatatc agcccgtttt ctgggtcaat aatgacaaaa tcaggatgcc
     5641 ggtttttccg tcccactgga atatcgtacc agacaaggca gtcatcattt aggaagctct
     5701 ccagtcgtga agccaccctc ttctctcctg gcgtcattct ctcctcagtg gacgccgata
     5761 tatttatcag tttcgccata aacgcacctt agttcttcct ctatttttta tatacgcttt
     5821 ttatggtgat ttcatgtgaa tcttccttat tttatttttt tgtgatatta atcacattta
     5881 acatggttgc ttgtttgtta acagtggaat caccttaacg gcagtgaaaa cagtaaacaa
     5941 atggcaatgt tcagaaatgg aaaaggccgc gaaagcgacc ttttcttgtt acagctgcaa
     6001 agcatttttt tgcgaggcgg cttcaggatt attgctgttc cagcgcatcg ccaattttga
     6061 ttttcgcctc tttacgcagg ttactcatca gagcttcaaa gacgatttgt gcgttgttct
     6121 gggtgatacc ctgcaccatc gcttttttct gatcttccgg cattgaacct tgtttcactt
     6181 catccagcgc cagcagaacc acattacctt gcatatcggt cgccataccg tagctcggtt
     6241 tgtctttcgc tggcagtggc agtgcaaacg cagcctggct aatcgggtca cgaccggaac
     6301 ggcttaaggt tttcggctcg ccaaatttca gaccggcagc ctgcatagct tccgcacctt
     6361 tgccggcttt caaatcaacc agcagtttct cagcatccac tttcgactgt tgttcagctt
     6421 tgttgtgctg aaccagtgcc ttaacttgtt cctgaacatc tgccaacggt ttcaccgctt
     6481 ccggtttgtg ctcgctgatg cgcagcacga atgcgcggtc gccgtctacg gtgatgatgt
     6541 cagagttgat gcccggcgcg ccgttttcac ctaccagacc gccgttgaag atagcgtcgg
     6601 caactggctt gaagttcaac tcttccggca ggttatcttt gctgaaccaa cccgtctgag
     6661 tggctttaac gccggcagct tgctctgcac cggccagaga ctcggtgtcg ttgcttgccg
     6721 catcgctcac tttctgctgc agcgcgtagt acgcatcgag ggctttttcg tgtttcactt
     6781 tcgccgcaat gtcgtcacgt acttcgtcta acgatttcac tttcgctggc tgaatgtcgt
     6841 ccagacgcac aatcaggaaa ccgaccgaag atttgataac accagacagt tggccttttt
     6901 ctttcagacc cgcatttttc agttcgtccg ggatagtggc atcttctaac caacccatat
     6961 caccgccgtt gcgggcagag ataatatcgg cagatttttc tttggctaat gcagcaaaat
     7021 caccgccctt attcagctca tcaagtaccg ctttcgcttc atcttcagtt ttggtctgga
     7081 tgatgctgta acgggtacgc tgcggctggg tgaattgatc ctgatgctga tcgtagtagc
     7141 tctggatatc cgcatcgcta accggttgct gcatcgtcgc ggcatccagc ttgatgtaac
     7201 tcacgcggaa ttgttccggc gtcatgaaat tgtttttgtt ttgttcgtag tagctggcaa
     7261 tttcctgttc ggtcacaggc tgcttcgccg ccagcgcgtt aacatcgata gtcgcttcac
     7321 gcaccacgcg ttgttgagct accagtgccg ccagctcgtc ggtttcacct ttcagcataa
     7381 aatcggtacc ggcaacgccg ttaatcagct gttgggtagt gagctggtta cgcagcgcct
     7441 gggcgtactg atcggcggtc atccccatct ggttgaggat accgttatag cggctgttat
     7501 caaatttgcc gtcaacctgg aaggctgggg tcgcgaaaat cgcctgttta acctgctcat
     7561 cgctgatacc cagtttcagc tcacgagcgt actgatccag aagcgcctcg tcgatcagac
     7621 gattcagcac ctgttgacgc agggttttca tatagccttc gttcgctgcc agctcggagt
     7681 attgatcgcc cagctgttgc tgcatgcgat tacgctcgct gttgaaggcg ttctcgaatt
     7741 gcccacggct gatttcctgg tcattcactt ttgcggcgta gttattgcct ccgccaatca
     7801 ggtaaccact cacgccggtc aatatgaacg acacgataat gataccgaaa ataatcttga
     7861 gcacgagact gtttgcagcc gtgcgtaagc tgtccatcat ggtgtaacaa cactccgctg
     7921 tagatgactg tgtacctcac gcaacgtatc gatgcgcgca aaggcctatt gtgacaagaa
     7981 acgggggcaa ttgtcagccc acaagacgca gaaagtcacc gaatacaaat aaaaaaggca
     8041 catcagtaga tgcgcccttg aacttcgtca catccccact ggggacaacg cttagtttac
     8101 cgcgtctttc agtgctttac ctgcacggaa gctcggtact ttagcagcag cgatggtgat
     8161 ctctttaccg gtctgcgggt tgcggccagt acgggcagca cgctctttaa cggcaaaagt
     8221 accaaaacct accagtgcta catcatcccc ttctttcaga gattcagtta cggaagcaat
     8281 aatagcatct aacgcacggc cagccgcagc tttagagata tcagcccctg cagcaatctt
     8341 gtcgatcaat tgagatttat tcactcttct cttcctcttt ataatttata tcgcacttga
     8401 atccttcaag gtacgaacgc gcagcagtta tatcaggcca gccatcccct tacaacaccc
     8461 cttaataagg gcaagcctga tccgccatct aacttagcga gacaaaaaaa ggctggcaag
     8521 cccgaattag cctgccagcc ctgtttttat tagtgcattt tgcgcgaggt cactattttg
     8581 cagtcacaac ctgcatgcca gacggttcat tttgtagcgc cagagtcaga acttcctcaa
     8641 tgcgcttcac aggatgaatg tccagatcgg caattacgtt gtcaggaatc tcttccagat
     8701 cgcgtttatt ttcgaacgga attaacactg ttttaatccc gccgcgatgc gctgccagta
     8761 gtttttcttt caaaccaccg atcggcagta cctgaccacg cagagtgatc tcaccggtca
     8821 ttgccacatc ggcacgaacc gggttaccgg tcaggcaaga aaccagcgcg gtgcacatgg
     8881 caataccggc actcggacca tctttcggcg tcgcaccttc cggtacgtgg acgtggatgt
     8941 cacgtttttc gtaaaaatca gggttgatcc ccagtttttc cgcacgcgca cgaaccaccg
     9001 ttaacgccgc ctgaatagac tcctgcatca cttcgccgag cgaaccggta taggtcagtt
     9061 tacctttccc cggaacgcac gcggtttcaa tggtcagcaa gtcaccgccc acttccgtcc
     9121 acgccagacc ggttacctgg ccgacacggt tttcgttatc cgcgcgccca tagtcgaaac
     9181 gctgaacacc gaggtagtca tgcaggttat cgccgttaat ttcgatatgt tttaatgact
     9241 tatcgagcag taactgctta accgctttgc gacacagttt ggagatttca cgctccagac
     9301 cacgcacgcc cgcctcacgg gtgtagtaac gaataatgcc gataatggcg ctatcgtcga
     9361 cggtcagctc accttttttc agtgcattac gttcaatctg cttcggcagc aggtgacgtt
     9421 tggcgatgtt cagtttttca tcttcggtat aaccggagag gcgaatcact tccatacgat
     9481 ccagcagcgg tgccggaatg ttcatggagt tcgacgtagc gacaaacatt acgtcgctga
     9541 gatcgtaatc cacttccagg tagtggtcgc tgaacgctac gttctgctct ggatccagca
     9601 cttcaagcag tgcggaggcc ggatcgccac gcatgtcaga agacattttg tcgatctcat
     9661 cgagcaggaa cagcgggttt ttcacgccca ctttcgccat tttctggatc agtttacccg
     9721 gcatagaacc gatgtaagta cggcggtgac cacggatttc cgcttcatca cgcacgccgc
     9781 ccagcgccat acggacatat ttacgcccgg tggctttggc gatggactga cccagggagg
     9841 ttttacctac ccccggcggc cctaccaggc agaggatcgg tcccttgatt ttgttgacac
     9901 ggctttgaac cgcaagatac tcaaggatac gatctttcac gcgctccaga ccataatggt
     9961 cggtatcaag gatttcctgc gcctgacgca ggtctttttt gaccttgcta cgcgcattcc
    10021 acggcacctg taccatccag tcgatatatc cacgcactac ggtcgcttct gccgacatcg
    10081 gagacatcat tttcagcttc tgcagctctg cttccgcttt ctcttttgcc tctttcggca
    10141 tcttcgccgc gtcgattttg cgcttcaggg cttcgttttc gtccggcgcg tcgtccattt
    10201 caccgagttc tttctgaata gctttcattt gctcgttcag atagtactca cgctgggatt
    10261 tctccatctg ctttttaacg cggttgcgaa tgcgtttctc aacctgcagc agatcgattt
    10321 ccgattccat cattgccatc agatattcca gacgttcgtt aacgtcggac atctccagaa
    10381 cggactgttt gtcagccagt ttcagcggca tatgtgcagc aatggtatcc gccagacgcg
    10441 ccggatcgtc gatgctattc agcgacgtca gcacttctgg tgggattttt ttgtttagct
    10501 tgatgtagcc ttcgaactgg ctgattgcag tacgcaccag cacttcctgt tcccgctcat
    10561 caatggtcgg cgactccaga tactccgcct tcgcagaaaa gtgttcgcca ttgtcagaga
    10621 gcgcagaaat acgcgcgcgc tgtaacccct cgaccagcac tttgacggtg ccgtcaggca
    10681 gtttcagcat ctgcaatata gaggccacgg tcccgacggt gaaaagatcg tttacacccg
    10741 gctcatccgt tgaagcttct ttctgcgcga ccagcataat ttttttatca tggtccatcg
    10801 ccgcttccag acaacggatg gatttttccc gcccgacaaa taaggggatg accatgtgcg
    10861 gataaaccac cacatcgcgc agcggcaata cggggatttc aatgcgttca gaacgctcag
    10921 gattcataga gctctctctt agtttaattt ccgccaggta atcagatgac acgactgtgc
    10981 ttcacgccat ctattaacat gtacgtcagt atatggggat gtttccccca cattcaacgc
    11041 cgagaataga ggaaaaatta aaggggagat aaaatccccc ctttttggtt aactaattgt
    11101 atggaaatgg ttaattattc accagatgcc tgttgcgctt ccggcttacc ataaatcagc
    11161 aacggtttgc tttgaccatc aattaccgac tcgtcgataa ccactttttc gacatcttcc
    11221 atggacggca gatcgtacat ggtatcgagc agtgcggctt ctacgataga acgcagacca
    11281 cgggcaccgg ttttacgcgc catcgctttc ttagcgatag catccagcgc ctcgtcacgg
    11341 aattccagat ccacgccttc cagattaaac agcgcctgat actgcttggt cagggcgttt
    11401 ttcggctctt tgaggatctg aatcagagct tcttcgctca gttcattcaa cgttgcgaca
    11461 accggcagac gaccaatgaa ctcagggatc agaccaaact tgatcagatc ttccggttca
    11521 acctgcgcca gcagctcgcc ttcgcttgct ttgtcggact tcgcttttac cgtcgcgcca
    11581 aaaccaatgc cggaaccggt ttctacacga tgggaaatca ctttatccag accggcaaac
    11641 gcaccgccac agataaacag gatcttagag gtatcaacct gcaagaattc ctgttgcgga
    11701 tgtttacgcc caccttgcgg cggaacagca gctaccgtac cttcgatcaa tttcaacagt
    11761 gcctgctgta cgccttcacc ggaaacgtct cgggtaatgg acgggttgtc tgacttacga
    11821 gaaatcttgt cgatttcatc gatgtagaca atcccgcgct gcgctttctg tacgtcgtaa
    11881 tcgcacttct gcaacagctt ctgaatgatg ttttcaacgt cttcgcccac ataaccggct
    11941 tcggtcagcg tggttgcgtc agccatggtg aacggaacgt ccagcaggcg cgccagcgtt
    12001 tcggccagta gcgttttacc ggaaccggtc ggaccgatca gcagaatgtt acttttgccc
    12061 aactcgacgc cattgctggt atctccgttg cgcagacgtt tgtagtggtt gtataccgcg
    12121 accgccagca cttttttcgc ctgttcctga ccgataacgt aatcgtccag gtggttgcga
    12181 atttcatgcg gcgtcggtag cgcactgcgt tcacgatgcg gtgcaacttc tttaatctct
    12241 tcgcgaatga tgtcgttaca taaatcaaca cattcgtcgc agatatacac ggatggaccg
    12301 gcaatcagct tgcgcacttc atgctggctt ttgccgcaaa aagagcaata cagcaatttg
    12361 cctgagccat ctttgcgttt atctgtcatg ggtcaaaacc tcttctttgt tctttgtgcc
    12421 gcacacgacg acgcaaatgc cattctcagg cgcaagccgc ttacagcgtt gtgccgccct
    12481 ggataagtat agcggcacag ttgcgcctct ggcatcaatt acgatgggtc agaatcgaat
    12541 cgaccagacc gtattccacc gcttcagggg cagaaaggaa gcgatcgcgc tcggtatcac
    12601 gttcaatctg ttctaatgat tgacccgtat gaagcgccat aagttcattc atgcgccctt
    12661 taactttcag aatttcacgg gcatgaattt cgatatcggt cgcctggccc tggtagccgc
    12721 ccaacggctg gtgaatcatc acgcgcgaat tcggcaggca gaaacgttta ccttttgccc
    12781 ctgcagtcag caagaaagca cccatcgagg ccgcctggcc catacagatg gtgctgacat
    12841 caggcttgat aaactgcatg gtgtcataga tagacatccc ggcagtaatc accccgcctg
    12901 gagagttaat gtacagatag atatcttttt ctgggttttc tgcttccagg aacagcatct
    12961 gcgccacaat caggttagcc atgtgatctt caacctggcc agtcagaaaa atgacgcgtt
    13021 ccttaagtag acgagaatag atatcaaaag agcgctcacc tcgtgaggtc tgttcaatga
    13081 ccatcggcac cagcgccata tggggtgcaa agttatctcg ttcgccgctg tatgacattt
    13141 ccgtctcctg gataaaattg aaaaaacctg ctgtaccgat tgtaacctta tggacggatt
    13201 atcagctagt ccctgtgtta tgggtacggc atcaccatat ttcaagcata acaatctttt
    13261 gttgttacgc taacaccgaa agaggctttt cgcacggttc catgcaaaat tcatgacaaa
    13321 aaaaagccca ccacctggcg gtgacgggcc tttgtgcgaa tttagagcgt tatactacgt
    13381 aaattacgcc tgctggttca tcagctcgtt gaaagtggtt tctttttcag tcactttcgc
    13441 tttcgccagt acagcttcaa cagcctgttc ttccagagca acattgcgca tgttgtccat
    13501 cagttctttg tttttgctgt agaactcgat aacttctttc ggatcttcgt acgcagaagc
    13561 catctcttcg atcaggcctt tcacgcgctc ttcgtcagct ttcagctcgt tggtgcggat
    13621 aacttcgccc agcagcaggc caacaactac gcggcgttta gcctgttctt cgaacagttc
    13681 gcgcggcagt tccagagctt gtttttcgtt gccaccgaaa cgctgtgcag cctggcgacg
    13741 cagaacgtcg atttcgctgt cgatcagcgc agccggtacg tcgatgtcgt tagcttttac
    13801 caaaccttcg atcgcctgag acttaacgcg gttacggatg gcgctcttca gctcgcgctc
    13861 catgttttta cgcacttcag cgcgcagacc ttctacggaa ccatcttcaa cgccgaaacg
    13921 tttgatgaat tctgcagtca gttccggcag ttcacgctct tcaactttct tcaggttgat
    13981 agcgaatttc gctgctttac ctttcaggtt ttctgcgtgg tactcttccg ggaaggtcac
    14041 gtcgatggtg aactcttcgc cagctttgtg gcctttgata ccgtcttcaa agcccgggat
    14101 catgcgaccc tggcccatcg ccagtacgaa atcagacgct ttaccgcctt cgaactcttc
    14161 gccgtctaca gaaccggtga agtcgatggt cacacggtct tctgcttcaa cagcgccgtc
    14221 tttttctttc caggttgcct gctgtttacg cagagtatcc agcatgccgt caacgtcagc
    14281 gtcggtcact tcaacgatcg gtttttcaac ttcgatcgct tccagaccct gcagttcaac
    14341 ttccggataa acttcaaact ctacagagta ggtgaagtct tcacccagct tgtattcgcc
    14401 cggaacatag gtcggtgcgc cagccggatt gattttttct ttaatgatgg cgtcaatgaa
    14461 gttacggctc atcaggtcgc ccaggacgtc ctggcgtaca gacgcgccat aacgctgagc
    14521 aacgatattc attggcactt tgcctttgcg gaagccgtca atacgtactt ttttcgcaac
    14581 gttgaccagc tcgcttttaa cagcggtctc gatgctgtca gcagcgatag taatcgttac
    14641 acggcggcca aggccttgag tggtttcaac tgaaacttgc atcttgttac ctcaaaaaat
    14701 cacagtgctc ggtcaactct tactccgcaa gcacaggttg ttggcttcgc ggaaccggga
    14761 tgttcttata atcaatcaca ttccctgtta ccagaatcat cccgaaagca ttcaaatagg
    14821 acgcagcatt atagcggcat cacttttatg agtcgagaac ggttattgcg cgctgtcgcg
    14881 tattttttca caattcccgg gcgattttcg tctgaatgct gggcaaaaag cgcaaaatcc
    14941 agacaaaaca aaacggcccg caggccgttt ttcataaaat ctttagcaac atactggaaa
    15001 agcgccgaca gttgcaaatg cgtttttacg cgatgcttcc tgctccacga cagggaggag
    15061 aggcaaagac ggtgtcctgc aacccttccc actccttaat agtgtaagta tgcagagcca
    15121 gcgcatgaac ggtagtagag agttcctccg ctaaagtact gtaaatcatt cgatgacgat
    15181 tcagaaaacg ttcacccgta aaacgatcgc tgaccagcac aactttaaaa tggctttcag
    15241 agccggctgg gacattgtga cgatagcttt catccactac ttcgaggaat acgggttgga
    15301 acgccgccct taatttttct tctatccgct cacgtatcat catgaaatat ctcctccgac
    15361 aacgctgaga tgtcatccat ccctttaaat actagccgct tttaccgttt ccattgcagc
    15421 ttaacaacaa atatttaggt ttcgtcctgg tttttccgtc aagcgtatga agtttactga
    15481 tttaaagatt gaaacacagg cctcgcatca gcgtttcttt taccaggggc gattatttcg
    15541 tcaacatgat gaatttacat gccttaccca cttcccctcg ccgttggcaa tgttatgatg
    15601 gcgggaattt ttaccaaaca cgcttaagag tctctgagag cccgaacatg ttcaaaaaaa
    15661 tcctcttccc gttagttgct ctgtttatgc ttgcaggatg cgcaaaaccg ccaacaacta
    15721 tcgaagtttc cccgacgatt acactgccac agcaggatcc aagcctgatg ggcgtcaccg
    15781 taagcattaa tggtgccgat cagcgtaccg atcaggcgct ggcaaaagtc acccgcgata
    15841 atcaaatcgt taccctgacc gcctcccgcg atctgcgttt cctgctgcaa gaagtgctgg
    15901 aaaaacaaat gaccgcccgt ggatacatgg ttggtccgaa tggcccggtt aatctgcaaa
    15961 tcatcgttag ccaactgtat gctgacgtgt cccagggcaa cgtgcgctac aacatcgcga
    16021 ccaaagcgga tatcgccatc atcgctaccg cgcagaatgg caacaaaatg accaaaaact
    16081 atcgtgccag ctacaacgtt gaaggtgcgt tccaggcctc caacaaaaat atcgccgatg
    16141 cggttaacag tgtgctgacc gataccatcg ctgatatgtc tcaggacacc agcatccacg
    16201 aattcatcaa gcagaacgcg cgttaatttc tgccctctgg cccggtgcaa gccgggcctg
    16261 tagacgccca tgtccagtca atatttacgt atttttcaac agccgcgttc agccatattg
    16321 ctgatcctgg gttttgcttc cgggctaccg ctcgccctga cgtccggcac cttacaggcc
    16381 tggatgacgg tcgagaatat cgatctcaaa accattggtt tcttctctct ggtaggccag
    16441 gcttacgttt ttaaattctt ctggtcacca ctgatggacc gctacacgcc cccatttttc
    16501 gggcggcggc gcggttggct gctcgccacg caaattctgt tattagtcgc cattgcggcg
    16561 atgggttttc tcgaaccagg cacccagctc cgctggatgg cggcactggc ggtggtgatc
    16621 gctttttgct ctgcctccca ggatattgtc ttcgatgcgt ggaaaaccga tgtgctcccg
    16681 gcagaagaac gtggtgcggg cgcggcaatc agcgtgctgg gttaccgttt agggatgctg
    16741 gtttccggcg gcttagccct gtggctggca gataaatggc tgggctggca gggcatgtac
    16801 tggttgatgg cggcgctgtt gatcccctgt attatcgcga cgttgcttgc accagaaccc
    16861 accgacacca ttcctgtgcc caaaacgctg gaacaagcgg ttgttgcacc tctgcgagat
    16921 ttctttggtc gcaataatgc ctggcttatt ttgcttctta tcgtgctgta taagctgggc
    16981 gacgcattcg ctatgagcct gacaaccacg tttttgattc gcggcgtcgg gtttgatgcg
    17041 ggtgaagtag gcgtggttaa caaaacgctt ggcttactgg cgaccattgt tggcgcattg
    17101 tacggtggga ttttgatgca gcgcctgtcg ctgttccgtg cactgctgat tttcggcatt
    17161 ttacaaggtg catctaacgc cggttactgg ctgctgtcga ttactgataa gcatctctac
    17221 agcatgggtg cagccgtctt tttcgaaaac ctctgtggcg ggatgggtac atcagccttt
    17281 gtcgcgctgt taatgacgct gtgtaataag tcattttccg ctactcaatt tgccctgctc
    17341 tcagcgcttt ctgctgtagg gcgagtttat gtcggccccg tggcgggttg gtttgttgaa
    17401 gcacacggct ggtcgacatt ctatctattc tccgtcgccg ctgccgtacc agggcttatt
    17461 ttgctgctgg tttgccgcca gacgcttgaa tatacacgag taaatggcaa ctttatctcc
    17521 cgtaccgaat atcctgcagg ttatgccttt gccatgtgga cactggcggc gggtatcagc
    17581 ctgttggccg tgtggttact gctgttgaca atggacgcgc tggatttgac gcacttctct
    17641 ttcctgcctg cgctgctgga agtcggggtt ttagtcgccc tttctggcgt tgtgcttggt
    17701 ggtttgctgg attatctggc gctacgaaaa acacatctga cgtaatctgt aaatattatt
    17761 tagctgtagt aatcactcgc cgtaattatt acggctaaat aaacatcagc atcgcttatt
    17821 aatatttgtt cttttgtgcg gcttagcgtt tggaaattat tgacgcaatt tatgaattct
    17881 atttttctta cacgattcag ctaatgagtc cttattttct caccacccag ttgtcacctc
    17941 aatgataatt atttgttaaa taattgtttt attttatatt gattatacca attgcccacc
    18001 caaactcctt ctgcactccc ctttcgttat aacgcccttt tgcaacagct tcttaaaatc
    18061 aacttgatat gttttgcaac atatgtgacc tgacagccaa atccaagtaa caggaattta
    18121 atcatgttta cagtaatgta accttcccgt aaaatgccca cacactttaa acgccaccag
    18181 atcccgtgga attgaggtcg ttaaatgaga ctcaggaaat acaataaaag tttgggatgg
    18241 ttgtcattat ttgcaggcac tgtattgctc agtggctgta attctgcgct gttagatccc
    18301 aaaggacaga ttggtctgga gcaacgttca ctgatactga cggcatttgg cctgatgttg
    18361 attgtcgtta ttcccgcaat cttgatggct gttggtttcg cctggaagta ccgtgcgagc
    18421 aataaagatg ctaagtacag cccgaactgg tcacactcca ataaagtgga agctgtggtc
    18481 tggacggtac ctatcttaat catcatcttc cttgcggtac tgacctggaa aaccactcac
    18541 gctcttgagc ctagcaagcc gctggcacac gacgagaagc ccattaccat cgaagtggtt
    18601 tccatggact ggaaatggtt cttcatctac ccggaacagg gcattgcaac cgtgaatgaa
    18661 atcgctttcc cggcgaacac tccggtgtac ttcaaagtga cctccaactc cgtgatgaac
    18721 tccttcttca ttccgcgtct gggtagccag atttatgcca tggccggtat gcagactcgc
    18781 ctgcatctga tcgccaacga acccggtact tatgacggta tctccgccag ctacagcggc
    18841 ccgggcttct caggcatgaa gttcaaagct attgcaacac cggatcgcgc cgcattcgac
    18901 caatgggtcg caaaagcgaa acagtcgccg aacagcatgt ctgacatggc agcgttcgaa
    18961 aaactggccg cgcctagcga atacaaccag gtggaatatt tctccaacgt gaaaccagac
    19021 ttgtttgccg atgtgattaa caagtttatg gctcacggta agagcatgga catgacccag
    19081 ccagaaggtg agcacagcgc acatgaaggt atggaaggca tggacatgag ccacgcggaa
    19141 tccgcccatt aaaggggttg aggaagaata aagatgttcg gaaaattatc acttgatgca
    19201 gtcccgttcc atgaacctat cgtcatggtt acgatcgctg gcattatttt gggaggtctg
    19261 gcgctcgttg gcctgatcac ttacttcggt aagtggacct acctgtggaa agagtggctg
    19321 acctccgttg accataaacg tctcggtatc atgtatatca tcgtggcgat tgtgatgttg
    19381 ctgcgtggtt ttgctgacgc cattatgatg cgtagccagc aggctcttgc ctcggcgggc
    19441 gaagcgggct tcctgccacc tcaccactac gatcagatct tcaccgcgca cggcgtgatt
    19501 atgatcttct tcgtggcgat gcctttcgtt atcggtctga tgaacctggt ggttccgctg
    19561 cagatcggcg cgcgtgacgt tgcgttcccg ttcctcaaca acttaagctt ctggtttacc
    19621 gttgttggtg tgattctggt taacgtttct ctcggcgtgg gcgaatttgc gcagaccggc
    19681 tggctggcct atccaccgct atcgggaata gagtacagtc cgggagtcgg tgtcgattac
    19741 tggatatgga gtctccagct atccggtata ggtacgacgc ttaccggtat caacttcttc
    19801 gttaccattc taaagatgcg cgcaccgggc atgaccatgt tcaagatgcc agtatttacc
    19861 tgggcatcac tgtgcgcaaa cgtactgatt attgcttcct tcccaattct gacggttacc
    19921 gtcgcgttgt tgaccctgga tcgctatctg ggcacccatt tctttaccaa cgatatgggt
    19981 ggcaacatga tgatgtatat caacctgatt tgggcctggg gccacccgga agtttacatc
    20041 ttgatcctgc ctgtattcgg tgtgttctcc gaaattgcgg caaccttctc gcgtaaacgt
    20101 ctgtttggtt atacctcgct ggtatgggca accgtctgta tcaccgtgct gtcgttcatc
    20161 gtctggctgc accacttctt tacgatgggt gcgggcgcga acgtaaacgc cttctttggt
    20221 atcaccacga tgattatcgc catcccgacc ggggtgaaga tcttcaactg gctgttcacc
    20281 atgtatcagg gccgcatcgt gttccattct gcgatgctgt ggaccatcgg ttttatcgtc
    20341 accttctcgg tgggcgggat gactggcgtg ctgctggcag taccgggcgc ggacttcgtt
    20401 ctgcataaca gcctgttcct gattgcgcac ttccataacg tgatcatcgg cggcgtggtc
    20461 ttcggctgct tcgcagggat gacctactgg tggcctaaag cgttcggttt caaactgaac
    20521 gaaacctggg gtaaacgcgc gttctggttc tggatcatcg gcttcttcgt tgcctttatg
    20581 ccactgtatg cgctgggctt catgggcatg acccgtcgtt tgagccagca gattgacccg
    20641 cagttccaca ccatgctgat gattgcagcc agcggtgcgg tactgattgc gctgggtatt
    20701 ctctgcctcg ttattcagat gtacgtttct attcgtgacc gcgaccagaa ccgtgacctg
    20761 actggcgacc cgtggggtgg ccgtacgctg gagtgggcaa cctcttcccc gcctccgttc
    20821 tataactttg ccgttgtgcc gcacgttcac gaacgtgatg cattctggga aatgaaagag
    20881 aaaggcgaag cgtacaaaaa gcctgaccac tatgaagaaa ttcatatgcc gaaaaacagc
    20941 ggtgccggta tcgtcattgc ggctttctcc accatcttcg gtttcgccat gatctggcat
    21001 atctggtggc tggcgattgt tggcttcgca ggcatgatca tcacctggat cgtgaaaagc
    21061 ttcgacgagg acgtggatta ctacgtgccg gtggcagaaa tcgaaaaact ggaaaaccag
    21121 catttcgatg agattactaa ggcagggctg aaaaatggca actgatactt tgacgcacgc
    21181 gactgcccac gcacacgaac acgggcacca cgatgcaggc ggaaccaaaa tcttcggatt
    21241 ttggatctac ctgatgagcg actgcattct gttctctatc ttgtttgcta cctatgccgt
    21301 tctggtgaac ggcaccgcag gtgggccgac aggtaaggac attttcgaac tgccgttcgt
    21361 tctggttgaa actttcttgc tgttgttcag ctccatcacc tacggcatgg cggctatcgc
    21421 catgtacaaa aacaacaaaa gccaggtgat ctcctggctg gcgttgacat ggttgtttgg
    21481 tgccggattt atcgggatgg aaatctatga attccatcac ctgattgtta acggcatggg
    21541 tccggatcgc agcggcttcc tgtcagcatt ctttgcgctg gtcggcacgc acggtctgca
    21601 cgtcacttcc ggtcttatct ggatggcggt gctgatggtg caaatcgccc gtcgcggcct
    21661 gaccagcact aaccgtaccc gcatcatgtg cctgagcctg ttctggcact tcctggatgt
    21721 ggtttggatc tgtgtgttca ctgttgttta tctgatgggg gcgatgtaat gagtcattct
    21781 aacgaaagca gcggcgcatc ccatggcagc gtaaagacct acatgacagg ctttatcctg
    21841 tcgataatcc tgacggtaat tccgttctgg atggtgatga caggggctgc ctctccggcc
    21901 gtaattctgg gaacaatcct ggcaatggca gtggtacaga ttctggtgca tctggtgtgc
    21961 ttcctgcaca tgaataccaa atcagatgaa ggctggaata tgacggcatt tgtcttcacc
    22021 gtgctaatca tcgctatcct ggttgtgggc tccatttgga ttatgtggaa cctcaactac
    22081 aacatgatga tgcactaaga gcggcggtta tgatatttaa gcaatacctg caagtaacga
    22141 aaccaggcat catctttggc aacctgatct cggtgattgg gggattcctg ttggcctcaa
    22201 agggcagcat tgattatccc ctgtttatct acacgctggt tggggtgtca ctggttgtgg
    22261 cgtcgggttg tgtgtttaac aactacatcg acagggatat cgacagaaag atggaaagga
    22321 cgaagaatcg ggtgctggtt aaaggcctga tctctcctgc tgtctcgctg gtgtacgcca
    22381 ccttgctggg tattgctggc tttatgctgc tgtggtttgg cgcgaatccg ctggcctgct
    22441 ggctgggggt gatgggcttt gtggtttatg tcggcgttta tagcctgtac atgaaacgtc
    22501 attctgtcta cggcacattg attggttcgc tctccggcgc tgcgccgccg gtgatcggtt
    22561 actgtgcggt aaccggcgag ttcgatagcg gcgcagcgat cctgctggct atcttcagcc
    22621 tgtggcagat gcctcactct tatgccatag ccattttccg ctttaaggat tatcaggcgg
    22681 caaacattcc ggtactgccg gtggtgaaag gcatttcggt ggcgaagaat cacatcacgc
    22741 tgtatatcat cgcctttgcc gttgccacgc tgatgctctc tcttggcggc tacgctgggt
    22801 ataaatatct ggtggtcgcc gctgcggtga gcgtctggtg gttaggcatg gctctgcgcg
    22861 gctataaagt tgctgatgac agaatctggg cgcgcaagct gttcggtttc tctatcatcg
    22921 ccatcactgc cctctcggtg atgatgtccg ttgattttat ggtgccggac tcgcatacgc
    22981 tgctggctgc tgtgtggtaa cacaacctct cagttaaaaa ggtgctacgg cacctttttt
    23041 cttagcatta gaatcatatc cctctcgaaa tatttactaa aaaatccgca tgtttacccc
    23101 attcgtttgc cgctttacac tagtcgcgaa tttaaaacag aggtggtaat gaacgattat
    23161 aaaatgacgc caggtgagcg gcgcgcgacc tggggtttag ggaccgtatt ttcattacgc
    23221 atgctgggca tgttcatggt tctgccggtt ctgaccacgt acggcatggc tctgcaaggt
    23281 gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca ggccgttttt
    23341 cagattccgt ttggcctgct ttcagaccgc attggtcgca aaccattaat tgtcggtggg
    23401 ctggcggtgt ttgccgccgg tagcgttatc gctgcgctct ctgactccat ctggggaatt
    23461 attctgggcc gggcgctaca aggctccggt gcgattgccg ctgccgttat ggcgctgctt
    23521 tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg cgtgagcttt
    23581 ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa acttgggctg
    23641 cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt gaccatttgg
    23701 gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt gaaaggcagt
    23761 ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat tatgtgtctg
    23821 catattttgc tgatgtcgac ctttgttgcc ctgcccggac agatggctga tgcggggtta
    23881 ccggcggctg aacactggaa ggtctacctg gcaacgatgt taatcgcctt tggctcggtc
    23941 gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt tgtcttctgc
    24001 gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca gttctggcaa
    24061 ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc ccttctgcct
    24121 tcacttatca gtaaagaatc gccagcaggt tacaaaggta cggcgatggg tgtttactcc
    24181 accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat tgacggcatg
    24241 tttgacggtc agggcgtatt tctcgctggc gcaatgctgg ccgcagtgtg gctggcagtc
    24301 gccagtacca tgaaagaacc gccgtatgtc agcagcttgc gcattgaaat cccggcgaac
    24361 attgccgcaa acgaggcgtt aaaagtgcgt ttgctggaaa ctgaaggcat caaagaagtg
    24421 ttgattgcag aagaagaaca ttcagcttat gtgaaaatcg acagcaaagt gacgaatcgc
    24481 tttgaggttg aacaggcaat tcgccaggca taaaagcgag gcgaaattgc ctgctgcgct
    24541 acgcttatca ggcctaccaa gttcctgagt tacaaacctt tgtaggccga atcaggcgtt
    24601 catgccgcat ctgacatgaa caaagcgcag gttgtcacga ttaatcgcgg aagtttttga
    24661 actggaacgg ctgaccgaga tcgccaccac gtaccatcgc catgacagcc tgtaaatcat
    24721 cacgagattt acccgttacg cggatctcat cgccctgaat ttgcgcctgc actttcagtt
    24781 tgctgtcttt gatcattttg acgattttct tctgagtcgc gctctcaatg ccctgtttca
    24841 gtttcgcttc cacaaaccag gttttaccgc tatgagcgat attttccggt acatccagtg
    24901 aactgccttc aatgccgcgc ttcagcaact tggcacgcag aatatccagc aactgattga
    24961 cctggaagtc ggactcgctc aacactttga tggttttgct ggcgtcattc agctcaaatg
    25021 aggcttcaac gttacggaag tcaaaacggg actccacttc gcggctcgcg ttatcgaccg
    25081 cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc atcttttcct
    25141 ctcccttcat ttttgatgcg aagcataata cctgcaaagt taaataacca acagtcctgc
    25201 accaggacgt atcccttaat tgtgcatagt cgcaacagca tgcacaatta agggatagcc
    25261 ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac cgtattggga
    25321 tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca cgacgttcag
    25381 ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac agatggttcg
    25441 atatttaacg aatcgctgac cgccaacgat cccgattttc tcgccaccag cgatctgctc
    25501 ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc gtccacactg
    25561 cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga agagttgcaa
    25621 aacattcagc agccattact gatgggcacc accacccatg cagctcgccg cgacggaaat
    25681 gtcattattc atgtggcaaa cggtatcacg catattggtc cggcacggca acaggacggc
    25741 gattacagtt atctggcgga tattttgcaa accgtgttac ctgacgtcgc ctggcataac
    25801 aatattcgcg ccgagctgtg gcgcaagctg gcagtcaact gtgtgattaa tccactgacc
    25861 gccatcttga attgcccgaa tggtgaacta cgccatcatc aacaagaaat tatgcagata
    25921 tgcgaagaag tcgcggcagt gatcgaacgc gaagggcatc atacttcagc agaagatttg
    25981 cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc gatgttgcag
    26041 gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct cttacgccgc
    26101 gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat ggtaaaaaga
    26161 aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta gtgaagagac
    26221 tgaagccgtc accactatcg acctcttggt tcgcggcggt atcaaagtca ccactgccag
    26281 cgtcgccagc gatggtaacc tggcgattac ctgctcgcgc ggcgtgaagc tgctggcgga
    26341 tgcaccgctg gttgaagtgg ctgatggcga atatgacgtg attgtgctgc ctggtggcat
    26401 caaaggcgca gagtgttttc gcgatagcac cctgctggtt gaaaccgtta aacagttcca
    26461 ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc tggtcccgca
    26521 cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca aaatacccgc
    26581 cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc tgaccagcca
    26641 ggggccaggt acagctatcg actttggtct gaaaattatc gacctgctgg ttgggcgcga
    26701 gaaagcccat gaagtggcat cacaactggt gatggcggca gggatttata attattacga
    26761 gtagtgtcgg atgcggtaaa cgtcgcatct gaccagatgc gacgttacaa ccacatatta
    26821 cgggcgatac accttcacat tgttaaaacc ctgctcgcgc agatagagcg cctgcaggcg
    26881 gctcatcacc ccgcgctcac accacaacag ccaggttttg ctctggtcga gatcgccaaa
    26941 tttggtgctc agtttataga acggcagaga aaccacatca atcccttcga ctttcagtgg
    27001 cttatcttcc tgttcatcga tagaacggat atcgaggatc acgtcgttcg ggccgaagcc
    27061 attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt cgcggatatc
    27121 aacgttattc gcttcctcca ccactttatc aagaatgctg aagtcgaact tctcttcttc
    27181 cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggaaatca cgccgcaata
    27241 ttccggcatc gtgcgggcaa agtcttcggt gccaatctgg cgagccaggt tgatgatgtg
    27301 ctctttgtcg taagagatca gcggacgcag gatcagcgta tcggagacgt tatcaatcag
    27361 gcgcaggttg gtcagcgtct ggctggacac ctggccgagc gcttcaccgg taaccagcgc
    27421 ctgtacgccg taacgttctg ccactttgga cgcggcacgc accatcatac gtttgaggat
    27481 aacgcccatc tgaccgtcgt cgattttctc gagaatttca ccgacgaccg gctcaaagtt
    27541 aatggcgaca aaacgcacgc ggtgggagct accaaaacgg ttccacagat aatgcgccac
    27601 ctgacgaaca ccaatttcat gcgccgcgcc gccgagatta aagaagcagt aatgcacgcg
    27661 gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac cggaaatgag
    27721 cgacaacaca tcttcctggg tgccgatcgg gaaaccgcca ataccttcgt agcggccttt
    27781 aatcagcagc agacgatcgt cttccacttc cagatggaca gtcacttccg gattggtcag
    27841 cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac gttccacatc
    27901 aatcgagcta aaatcatgtt tgccgcggcg cttcacgcgt acgcagaagg ttttgccttc
    27961 cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt ctgtaaacgg
    28021 cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg cgtcgcgaat
    28081 agccagacgc tggttttcat cttttgcgcg aacttcgatg ttatcccagt ggcggacgac
    28141 agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa ggatttttat
    28201 aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt taatgataaa
    28261 cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg aaaaatacag
    28321 cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc gctttgctgc
    28381 gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt accatgacgg
    28441 ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg ctcccgccag
    28501 ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa gtggcgacct
    28561 gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac gtcaggggca
    28621 ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca atgaagacgc
    28681 ctctctaacc ccttttacac cggacaatga gtaatggact ttccgcagca actcgaagcc
    28741 tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc ctttcagaac
    28801 actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg cctgcgacct
    28861 ttcctggttt atgccactgg tcatatgttc ggcgttagca caaacacact ggacgcaccc
    28921 gctgccgccg tagagtgtat ccacgcttac tcattaattc atgatgattt accggcgatg
    28981 gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg cgaagcaaac
    29041 gcgattctcg ctggtgacgc tttacaaacg ctggcgttct cgattctaag cgatgccgat
    29101 atgccggaag tgtcagatcg cgacagaatt tcgatgattt ctgagctggc gagcgccagc
    29161 ggtattgccg gaatgtgcgg tggtcaggca ttagatttag acgctgaagg caaacacata
    29221 cctctggacg cgcttgagcg tattcatcgt cataaaaccg gcgcattgat tcgcgccgcc
    29281 gttcgccttg gtgcattaag cgccggagat aaaggacgtc gtgctctgcc agtactcgac
    29341 aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct ggatgtggtg
    29401 ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg taaaagtacc
    29461 taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct gatcgacgat
    29521 gcccgtcagt cgctgaaaca actgactgaa cagtcactcg atacctcggc actggaagcg
    29581 ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag gcccctgatg
    29641 agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca ggagttacga
    29701 ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta tttactcgac
    29761 agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga actgaccgtg
    29821 gcgctgcact atgtctacaa taccccgttt gaccagttaa tctgggatgt ggggcatcag
    29881 gcttatccgc ataaaatttt gaccgggcgc cgcgacaaaa tcggcaccat ccgtcagaaa
    29941 ggtggcctgc acccgttccc gtggcgcggc gaaagcgaat atgacgtatt aagcgtcggg
    30001 cattcatcaa cctccatcag tgccggaatt gggatcgcag ttgctgccga gaaagaaggc
    30061 aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttactgctgg catggcgttt
    30121 gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggttct caacgacaat
    30181 gaaatgtcga tttccgaaaa tgttggcgcg ctcaacaacc atctggcgca gctgctttcc
    30241 ggtaagcttt actcttcact gcgcgaaggc ggaaaaaaag ttttctctgg cgtgccaccc
    30301 attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt gcctggcacg
    30361 ttgtttgaag agctgggctt taactacatc ggcccggtgg acggtcacga tgtgctgggg
    30421 cttatcacca cgcttaagaa catgcgcgac ctgaaaggcc cgcagttcct gcatatcatg
    30481 accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc caatcacctt ccacgccgtg
    30541 cctaaatttg atccctccag cggttgtctg ccgaaaagta gcggcggttt gccaagctat
    30601 tcaaaaatct ttggcgactg gttgtgcgaa accgcagcga aagataacaa gctgatggcg
    30661 attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa attcccggat
    30721 cgctacttcg acgtggcaat tgccgagcaa cacgcagtga cctttgctgc gggtctggcg
    30781 attggaggct acaaacccat tgttgcgatc tactccacct tcctgcaacg cgcctatgat
    30841 caggtgctgc atgacgtggc gattcaaaag cttccggtcc tgttcgccat cgaccgtgcg
    30901 ggcattgttg gtgctgacgg tcaaacccac cagggcgctt ttgatctctc ttacctgcgc
    30961 tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg ccagatgctc
    31021 tataccggct atcactataa cgatggcccg tccgcggtgc gctacccgcg cggcaacgca
    31081 gttggcgtgg aactgacgcc actggaaaaa ctgccaattg gcaaaggcat tgtgaagcgt
    31141 cgtggtgaga aactggcgat ccttaacttt ggtacgctga tgccagaagc ggcgaaagtc
    31201 gctgaatcgc tgaacgctac gctggtcgat atgcgtttcg tgaaaccgct tgatgaagcg
    31261 ttaattctgg aaatggccgc cagccatgaa gcgctggtta ccgtagaaga aaacgccatt
    31321 atgggtggtg caggcagcgg cgtgaacgaa gtgctgatgg cccatcgtaa accagtaccc
    31381 gtgctgaaca ttggtctgcc ggacttcttt attccacaag gaactcagga agaaatgcgc
    31441 gccgaactca gcctcgatgc caccggtatg gaagccaaaa tcaaagcctg gctggcataa
    31501 tccctactct actcctgcta tgcttaagaa attattcatg gcaggagtgg cagcatgcaa
    31561 tacaacccct taggaaaaac cgaccttcgt gtttcccgac tttgcctcgg ctgtatgacc
    31621 tttggcgagc cagatcgcgg taatcacgca tggacactgc cggaagagag cagccgtccc
    31681 atcattaaac gtgcgctgga aggcggcata aatttctttg acaccgccaa tagctattcc
    31741 gatggcagca gcgaagagat cgtcggtcgc gcactgcggg atttcgcccg tcgtgaagac
    31801 gtggtcgttg cgaccaaagt gttccatcgc gttggtgatt taccggaagg attatcccgt
    31861 gcacaaattt tgcgctctat cgacgacagc ctgcgccgtc tcggcatgga ttatgtcgat
    31921 atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct ggaagccctg
    31981 aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat gcacgcttcg
    32041 cagtttgctc aggcactgga actacaaaaa cagcacggct gggcgcagtt tgtcagtatg
    32101 caggatcact acaatctgat ttatcgtgaa gaagagcgcg agatgctgcc gctgtgttat
    32161 caggaaggcg tggcggtgat tccatggagt ccgctggcgc ggggccgtct gacgcgtcct
    32221 tggggagaaa ccaccgcacg gctggtgtcg gatgaagtgg ggaaaaatct ctaccaagaa
    32281 agcaatgaaa atgacgcaca aattgcagaa cgcttaacgg gcgtcagtga agaacttggt
    32341 gcaacgcgag cacaagttgc gctggcctgg ttgttgagta aaccgggcat tgccgcaccg
    32401 attatcggaa cttctcggga ggaacagctt gatgaactgc taaacgcggt ggatatcacc
    32461 ttgaagccgg aacagatcgc cgagctggaa acgccgtata aaccgcatcc ggtcacagga
    32521 tttaaatagg tatgcaggcc tgatgagacg tgacaagcgt cacatcaggc atcggtgcac
    32581 aactacgaca gaatacccag cggccagtga tgaccgataa aatacaggat gccagcggag
    32641 atcaccccgg cgacaatatc gtcaatcatg atccccatgc cgccatgtac attgcgatca
    32701 aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac aaatccggcg
    32761 gtaacccact gccagtcatt ggtcggcagc gccatgagcg taatccacat accaataaat
    32821 tcgtcccaga caatactgcc gtgatcgtgc acgcccatgt ctttcgccgt ttgatgacaa
    32881 agatagacgc cgatacagat ccccagcatt accaccagcg aatagagctg ccagggcaaa
    32941 aaagtcatca gataccagaa gggaatcgcc gccagcgacc ccatcgtgcc aggaacgatc
    33001 gggcttaatc cacttccgaa tccgacagca agcagatgcc acggattact cattttcagg
    33061 cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc agtctaacgt
    33121 gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca tttgcccgat
    33181 acaggtaaac ggtacgccca ggtgtccgag agccacatcc agcgcgccac ggttcagttc
    33241 cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc gcagcgcctg
    33301 ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca ggtcaatacg
    33361 tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac cgtcagagag
    33421 atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac gcggcgacgg
    33481 acggagatga cgtttgatca agtagtccgc atctttagca tcggcaacct gcaaacggtt
    33541 ttgcaaaatc gccagcccgg cagcgctatc gcccggtgta ccggtcacat agatccagtc
    33601 acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc cgtggatacc
    33661 caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca tatcgtaata
    33721 attgagaaga tcaaacaagc tgtcgctgaa ggactcaagc cacgcttcgt ctacgtccgg
    33781 taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca gatcgcttag
    33841 gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga ggaagtggtt
    33901 gcccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcaggga tattgagaag
    33961 agcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta cacggtcaaa
    34021 ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag caggtaaaaa
    34081 agaaaaggcc ggcgatcatg gaacggtctt ccgtgaatct accggcctgg atattacttt
    34141 ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa cttatggctg
    34201 tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac tttgtatggc
    34261 acatcgctac gtttagacag ctcgtacagc gcaatgcgca gtactgcttt ttctacctgg
    34321 cccagttctt ccagcagacg ggacaggtac ggcttcatca gtccgtcgag gtatgcggtg
    34381 ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc ttttacatcc
    34441 tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa ctgccaggag
    34501 tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt cacggatttc
    34561 cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa gcgcggtcag
    34621 tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg cttgttcaat
    34681 gctttcagtg gtcagaacac caaaagcaac cggaatttcg ctgtcctggg caacatgcgc
    34741 caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac cacgaataac
    34801 cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac ccgccgccag
    34861 cggcagctca taggcacctg gcacccaaac aacggtaatg ttttcatctt ttacctgacc
    34921 gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa agttgttgaa
    34981 acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt caataatgtt
    35041 cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta gcataatatt
    35101 tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc aggcaaacat
    35161 ccgggcctac atgacgtatc tctttgaatt taaattgggg agcgtcggct aatttctcaa
    35221 gccctggcag cgtgcataat ccacgggcgt cgttgcctaa tagtttaggt gcgatataga
    35281 caatcagctc atcgactaaa cccgcctgta gcaatgcgcc agcgagcgtt ggccccgctt
    35341 ccacccagat gctgttaatc tgctgtttac ccagttgcat cattagtaca accagatcca
    35401 ggtgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac tcacgagaat
    35461 cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga tgttccggcg
    35521 tcacgcgatt ttggctatca atcacaatac gtaccggctg gcggagattt tgttgcgggt
    35581 agagcccctg agtttgttca tccagttcag accaacgcac tgttaaggct ggatcatccg
    35641 ccagcaccgt ggcgctgctg gttaaaatgg catgactttg cgcgcgcagt cgttgtacgt
    35701 cgcgtcgcgc ctgaggcgaa gtgatccact ggctttcgcc actcgccatc gccgtgcgac
    35761 catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc atccgcttga
    35821 gaaagccttt attcaattgc tcagcttcac tcatcatcag cccgtggctg acgtcaatgc
    35881 cagcctgttg cagacggtaa agtccacgtc cagcgacctg cggattgggg tcttgcattg
    35941 cagcaaccac gcgcgccacg cccgcagcaa ttaatgcatc acaacacggt ggcgtacgac
    36001 catgatggct acagggttcg agtgtgacat acgccgttgc gcctttggct ttttcgccgg
    36061 ccatgcgcaa cgcgtgtacc tcggcatgag gttcacccgc tcgttgatgg taaccttcac
    36121 cgacaatttc gccatctttg acaatgacgc acccgacatt cgggttggga tgcgtggtaa
    36181 aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc tgcacggctt
    36241 agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa ctgcggtaga
    36301 cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc atcaccagat
    36361 tgccaatcat cttgctcggc acttcgcgct caccggtggc gcgcagctgc gattttatat
    36421 gattgattgc catttcgacg tcatcggaac tcaccggacg tttttccagc gcccgcagca
    36481 ttccgctacg taatttctct tcattaaacg gttcacgcac gtcgttgctt tttacaacac
    36541 gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac accagacact
    36601 gccgacggcg gcgtacggat gaaccctcac ccacgagacg agagtcaatt accttagtgt
    36661 ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt aacagaaatc
    36721 tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata aaggatcgat
    36781 gctttcactg cttttaccct ctaccattag gctaattgcg acgagaaagg gacgacctat
    36841 gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta gcgcctgtgt
    36901 gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc aggagatgac
    36961 gcaactcaat aaagaaacgg tcaaaatcac tcagcaaaac aagctgaatg caaaatccag
    37021 cagtggggtt taccttctgc ctggagcaaa aacaccagca agactggaaa gccagatcgg
    37081 tactttacgt atgtcgctgg tgaatattac gcctgacaca gatggcacta ccctgacgct
    37141 acgtattcag ggcgaatcca atgacccttt acccgcattc agcggaaccg ttgaatatgg
    37201 gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc aattgattaa
    37261 cgcacctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacagt taaagggtat
    37321 gtctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta tgcagtaaac
    37381 gtattgcggg gcgatattgt gcgtcccgca acatctttct ccgtcatttt gttactccgc
    37441 ttacatcacc tggattgata gtaaaagttt gcaacaaggg tgaaagtcag tacaatcccc
    37501 gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac tgtgaaacga
    37561 aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga aatatttaga
    37621 aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc atacatatga
    37681 aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt actgtcaacg
    37741 cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc gttaacgttg
    37801 tcggaagcta tcacacccgt ttcggaccgc agctccgcaa cgatacctac cttgagtacg
    37861 aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg ccggtattct
    37921 tcggcggtaa ctccgatgca aaaggtatct ggaaccacgg ttctccgctg tttatggaaa
    37981 tcgaaccgcg tttctccatt gacaagctga ccaatactga ccttagcttc ggtccgttca
    38041 aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa gatggccgcc
    38101 agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg atgagcctgt
    38161 ccatgaacgt ctatgcgaaa taccagtggc agaactacgg cgcagcgaac gaaaacgagt
    38221 gggacggtta ccgtttcaaa gtgaagtact ttgtgccgat taccgatctg tggggcggcc
    38281 agctgagcta catcggcttc accaacttcg actggggttc cgacctgggc gatgacagcg
    38341 gttatgcgaa caacggtatt aaaacacgta ccaataactc tattgcgtcc agccatattc
    38401 tggcgctgaa ttacgatcac tggcactact ctgtcgttgc ccgttactgg cataacggtg
    38461 gtcagtggaa tgacgatgca gaactgaact tcggcaacgg caacttcaac gttcgctcta
    38521 ccggctgggg tggttatctg gtagtaggtt acaacttcta attgtgagaa tgccggattt
    38581 catatccggc attttttatc acttcttctt gttcatcatc gctttcaaat cagcgaatgg
    38641 gttgtacttc gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc
    38701 atactgatcc gcttcggtgt atttcgaatg ctcatgatcg tggcaataga gacaaagtaa
    38761 ttcccagtta ctgccatctt ccgggttatt agtatggtcg tgatcaatgt ggtgaacggt
    38821 aagttcacgc aggttggaat aaacaaactc gcgggaacag cgaccgcaga cccacggata
    38881 gatttttaat gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat
    38941 agccatgaaa aagttacctg gatgaaatgt agggttacgc gcagtttccc aagatgcggg
    39001 gattttcgca atcaccttac atcaaaaaaa atcccgatct tctgaccggg attttcaaca
    39061 tcagctgaaa cttgattaag gcagaattga cggctgatcc gccccttctt tttccacttt
    39121 ctgctgcaac atgtgttcgc gcttcatacc cagtttcaga gccagcgcag atgccacata
    39181 gatggaagat gcagtaccga tggaaacacc gataagcatg gtcaatgaga acccttccag
    39241 caccgggcca ccgaagaggt acaacatcag gataaccatc aaggtagtac cagatgttat
    39301 caaggtacgg tgcagcgtct gggtcaagga cacgttaaag atttcgtaag gcgtaccgcg
    39361 acggatcttg cggaagttct cacgaatacg gtccgatacc acgatactgt cgttaagtga
    39421 gtaaccgata accgacatca acgatgcaac gatggtcagg tcaatctcaa tatggaataa
    39481 cgacaaaata cccaacgtaa tgataacgtc gtgcgccagt gcaataacga cccctgccgc
    39541 cagtcgccac tcaaagcgga aaccaacgta cacaaggata gaaagcagcg ccgccatcaa
    39601 cgccatcgca ccggtttgcg caaggtctgc ccccacgctc ggaccgacga actcaatacg
    39661 cttcactgcc gcattctgat tggtggattc gttaatcacc ttcagaacct ggctgcccag
    39721 cacctgaccg ccggtttcgc cttcagcagg cggcatacgg accatgatgt cgtggctgct
    39781 accaaagttt tgcagcatcg gctcttcaaa accggctttt tgcaatgcat cacgcattac
    39841 gtcaatttca gccggttttt cgagcgtaat ttcaataacc gtaccaccgg taaaatccag
    39901 cccccagtta aagccgcgca cgcccataat aacgatagcg gcgattaaca gcagaccaga
    39961 gatgccgaaa gcccagtagt cccagcgcat aaagtcatag actttacggc cgtggtttag
    40021 ttgttcaaca gtatattcct gtgccacatc gcactcctca gattgacagc ttcttgacgc
    40081 gcttgccgcc atatagcagg tttacgatgg cacgcgtacc gacaatcgcg gtaaacatcg
    40141 acgtcgccac accgataccg gtagtaatcg cgaacccttt aattgccccg gtacccactg
    40201 catacaggat gatgacttta atcagcgtgg tgatgttcgc atcgaagatg gaactgaatg
    40261 cgccacgata acctttatca attgcctgtt gaacagtacg tccgttgctc aactcttctt
    40321 taatacgttc gttgatcagt acgttcgcat cgaccgctac cgcaagggtt aagacgatac
    40381 ccgcgatacc tggcatactc agcgttgcgc ctggcaacag cgacataatg ccgacgatca
    40441 agatcaagtt ggcaatcaga gcactggtcg caatcagacc aaacttctta tagaagatga
    40501 tcatgaacag aatagaaacc agcagaccgg caaggcaagc ctccagcccc tgctcaatgt
    40561 tctgcatacc cagggttggg ccaatggtac gttcttcaac aatctgaatc ggcgcgatca
    40621 acgcaccggc acgcagcagc agtgaaagct gacgcgcttc gcttgggtta ttgatgccag
    40681 taatacggaa gctgttaccc agacgagact ggatgttggc gatattaatc acctcttcct
    40741 gtttcaccag aaccgcacga ccgtttgcat ctttcttacc gctgtctttg tactccacaa
    40801 acagagttgc catcggtttg ccgatgttgt ccttggtgaa gttagacatg atgttaccac
    40861 ccgcgctatc gagcgagatg ttaacctgcg gctggttgta ttcgtcctgg ctggaagtgg
    40921 agtcggtgat atggtcaccg gtcagaatta cgcgtttgta cagcacaacc ggctgacctt
    40981 cgcgggtctg tttcacttca gagtcgcccg gtacgcggcc agatgcagca gcggcctgat
    41041 caacgttggt gtttaccaga cggaattcca gcgttgccgt cgcacccaga atctctttcg
    41101 cacgcgcagt gtcctgaata cctggcagtt caacaacgat acggtcagca ccctgacgct
    41161 gaaccaccgg ctccgccacg ccaagttggt ttacacggtt acgcaggata ttgatgttct
    41221 gctgcaccgc atattcacgc gcttcactca gacgagcatc gctcattacc gcacgcagct
    41281 ggttgctgcc ctggctgcta atcaccaggt ccggatggcg cttgctcaga tacgcaatgg
    41341 cttcatcacg agctttagca tcgcggaaag tgatgctcag gccgtagttg ttttctttac
    41401 gaacggtggt atacgggatg cccttttcac gcaggtcgct gcgcaggcta tcgatatttt
    41461 gttcctggag tttgccaagc gcggtgtcca tatccacttc catcaggaag tgaacgccgc
    41521 cacgcaggtc aaggccgagc ttcatcggct cagcatgaat agctgccagc cagcgcggcg
    41581 ttgccggggc aaggttaagc gccacgacgt atttgtcacc cataacgccc attaatgctt
    41641 cacgagcgcg cagctgggtg tcagtggagt cgaagcgcgc aagaatagcg ccctcttcca
    41701 gtgccacaga cttagcagtt attttttctt cttgtaacgt tttctggacc tggatcagcg
    41761 tttgctcact ggcggcgact ccgcgcgcac cagtgatctg aacagccgga tcctcaccaa
    41821 acaggttggg aagcgcatac agcagaccga tgacaatcac cacgatcagc atgacgtact
    41881 tccacaaagg ataacggttt aacacggcaa ttcccttagg gaaaaatttt aattacagcg
    41941 ccttcatggt gcctttcggc agaacggcag ctacgaagtc acgtttaata actacttcag
    42001 tggtgtcatt cagtgcgata gcaatgtagc cgttttccgc tactttggtt acgcgaccga
    42061 ccaggccacc gttcgtcagc acttcatcac ctttagcgat ggagtccatc agctttttgt
    42121 gttctttggt gcgcttctgc tgtggacgca ggatcatgaa atagaaaatc agaccgaata
    42181 ccaccagcat caaaatcaaa gacatcgggc taccttgcgc cggtgcaccc gttgccgcta
    42241 ccgcatcaga aataaaaaag ctcattaaat ttccctcatt attaatatta atcaacgttc
    42301 aaaggtggta cttctcgccc ctgacgctgg taaaaatcag ttacgaagct ctctaattta
    42361 ccctcttcaa tagccttgcg taaacccgcc atcaaacgct ggtagtaacg aaggttatga
    42421 atggtgttga gtcgcgcgcc taatatttcg ttgcaacggt cgagatgatg caagtaagcg
    42481 cgtgaataat tgcgacaggt gtagcaatca cactcaggat cgagtgggcc agtatcgctc
    42541 ttatacttcg cattgcggat tttcaccacg ccatcggtca cgaacaaatg accatttcgg
    42601 gcgttgcggg ttggcattac gcagtcaaac atatcgatac cgcgacgtac gccctcaacc
    42661 aggtcttctg gtttaccaac gcccatcagg taacgcggtt tgtctgccgg aatttgcggg
    42721 catacatgct ccagaatgcg gtgcatatct gctttcggct cacccacagc cagaccgccg
    42781 acagcgtagc catcaaaacc gatatctacc agacctttaa cagaaatatc acgtaaatct
    42841 tcgtaaacac tgccctgaat gataccaaac agcgcatttt tgtttccgag actgtcaaaa
    42901 cgctcacggc tacgcttcgc ccaacgcaga gacatctcca tggagcgttt tgcgtaatcc
    42961 cagtcagcag gatacggcgt acactcatca aagatcatga cgatatccga accaagatcg
    43021 tactgaatct ccatcgactt ttccgggtcg aggaaaatcg ggtcgccgtt gatcgggttg
    43081 cggaagtgaa cgccctgttc ggtgatttta cgaatatcac caaggctgaa gacctggaag
    43141 ccgccggagt cggtaaggat tggtcctttc cactgcataa aatcgtgcag atcgccgtgc
    43201 agtttcatga tttcctggcc cgggcgcagc cacaggtgga aggtgttgcc gaggataatt
    43261 tgcgcgccag tggcttcaac ttcttccggc gtcatccctt ttacggtgcc gtaagtgcca
    43321 acaggcataa aacaaggcgt ttccactacg ccacgatcaa agaccaggcg gccacggcgt
    43381 gcgcgaccgt cggtggtgtc cagttcaaat ttcatttttt ctccactacg tcagaaaaac
    43441 agtccaacgt ttaaaccagc gccgcggaat tactccccaa cgcgctcatt aattgcctgc
    43501 ggattgtacg tgataaacat cgcatcaccg taactaaaaa agcgatattt ctcttctacc
    43561 gccgctttat aggcattcat ggtgtgttga taaccagcaa atgccgaaac cagcataatc
    43621 agcgtcgatt ccggaaggtg gaagttcgtc accagcgcat cgaccacttt gtactggaag
    43681 cccggataga taaagatttg ggtatcgtca aagaacggtt caatgagatc gtttttcgct
    43741 gcctgagccg cactttccag cgaacgtact gaggtggtac caaccgcaat cacccggtta
    43801 ccgcgcgctt tcgccgccag taccgcgtct accacatcct gcggcacttc agcgtattcc
    43861 gagtgcatga tgtgatcttc aatggtgtcg acgcgcaccg gctgaaaggt gcccgcacca
    43921 acgtgcaacg tcacaaacgc catctccacg cctttggcgc gtaatttttc cagcaaaggt
    43981 tcgtcaaagt gcagtccggc ggtcggtgct gctaccgcgc cgggtttttc gctataaacc
    44041 gtttgataaa gttcgcggtc tgcatcttcg tccggacggt cgatgtacgg cggcagcggc
    44101 atatggccga tgctgttgag aatatccagc accgaacgtt gatcattaaa ttcgacttca
    44161 aacagtgcgc cgtggcgcgc ggtcattgtt gcgttaatac tttcgtcatc gcccagcagc
    44221 agttctgcgc caggttttgg cgctttcgag gcgcgaatat gcgcaagaat gcgtttgtcg
    44281 tcgagcatcc gctcaaccag cacttcaatc ttgccgccgc tggctttacg cccaaacaga
    44341 cgcgccggga tcacgcgggt attattaaaa accagaagat cgccggggtt gagcttatcg
    44401 agtaaatcgg tgaaagtacc gtgcgtcagc gcgcccgtcg gcccgtccag cgacagtaaa
    44461 cgacagctac tgcgttcagg catgggatag tgggcaatca gggattcggg caattcaaaa
    44521 gagaaatcgg taacgcgcat gacactgact cagactaaaa taagaggcgg gtagtctagt
    44581 gccggggcgc tctccctgca acctttaccc ccctttggat agaatataat cgatgaattt
    44641 tttagctcac ctgcatttag cccatctcgc ggaaagctcg ctttccggca atttactggc
    44701 tgatttcgta cgcggaaatc ctgaagaaag ttttccgccc gacgtcgtgg ctggtattca
    44761 tatgcatcgg cgaatcgacg tattaaccga caatctgcca gaagtccgcg aagcgcggga
    44821 gtggtttcgt aatgaaacgc gccgtgtcgc gcctattacg ctggatgtga tgtgggatca
    44881 ctttctgtcc cgccactggt cgcagctgtc gcccgatttt ccgctacagg aatttacctg
    44941 ttatgcccgg gagcaagtga tgactattct tccagactca ccgccacgct ttatccatct
    45001 gaacaactac ttatggtcag agcagtggtt ggtgcgctat cgcgatatgg atttcatcca
    45061 gagcgtgtta aacggcatgg caagccgccg cccacgtctg gatgccctgc gtgactcctg
    45121 gtacgattta gacgcccatt acgatgctct cgaaacccgc ttctggcagt tttatccgcg
    45181 gatgatggag caggcgtcac gcaaggcgtt ataagacgtt aattcatcca caccgtagcc
    45241 gaaatggcag gcaaagccag aatcccgtcc gtcagttgcc cgtgaccttc tttgcgttgc
    45301 cattgcgcga cattaagcaa cggtgacgcg ggtagcacca cttcacaggc ttcgccacgg
    45361 ttgattgcca ccagtacacg ctgctgattc agcacgcgga caaataccac cacgttatct
    45421 tccgcataca gcacctgaca gccgccacga cgcaacgcct gacctttctt acgcagcgca
    45481 atcattcgct ggtacagcgc gaataacgcc gaatcctgct tttccacctg ccaggggaac
    45541 ggcttacggc aaaacggatc gtttttgcca tccagtccca cttcatcacc gtaataaatg
    45601 cacggtacac caggccaggt gaacagccag accaccgcca ggggcaggcg cgcaacatcc
    45661 cgaccgagca gcgttttaaa tcgcgcagta tcgtggctgt cgagctgatt aaacatacgt
    45721 aattgttgtt gatgagaaag ccctgcgcgg taattatcca tccaggccat acaggtttgg
    45781 gcgtcaattt gctgcggatc gtaagagata tcggtgttgg caagaaatcc ccacaacggg
    45841 aatgtgaagc cgcgatagtt catggcggca tcttccacat cggcctgtaa ccattgcctt
    45901 gcatcgccaa aatgttcgcc gacaatatac gcatccggct gggtttcttt cgccgcttcg
    45961 gtgatcccgg taacgtgctg caaattattg cgcgccccac ccgcttcccc cagcatatgc
    46021 accacatcca gccgccagcc atccatattc cacggcgctt tcagccagtg gcggacaata
    46081 ctgtcttccc cgcgataaat ttcattcacc aaactttccg actgataatc cagcttcggc
    46141 aagctggcat agcccagcca gtcgagcgcc gtgccatcat cactaaacga gtaccagtca
    46201 cgccagggtg attcggggtt gtgacaagct ccccccgtgc cacgattgtg cctgtcaaac
    46261 caggcatggg aatcgccact gtggttaaac acgccgtcca gcaccagtcg tattcccagc
    46321 tgctgcgtat tgtgtcgcaa acgcagcaac gccccatcac cgccaaactg cggatcgaca
    46381 tggcgataat cctcggtatc gtatttatgc acgctgggag ctttaaacac cggattgaga
    46441 tacagcgccg tcacgccaag ttttttcaga tacggcagtt tttcacttat cccgtccaga
    46501 tcgccgccat agaacgttga tccgccagcc tgcgccgtga ccggttcatc ccagtcacgc
    46561 aatatgatct cttgtccggc tgcgtgatgg taatagacat gatcctgttc agcttcacga
    46621 ggaagattac gggcgaaacg atcagggaag atctgataaa aaatctgatc cgcagcccat
    46681 tgtgggccga tatccggtac atcaatggca aactgctcca gtcgtgctgg cggcattcgg
    46741 ctgaagcctt gcggtgtaaa ccaacgctgg cgatcgtgcc acagcaattt gaaactataa
    46801 cgccgccgag gttgtccgct ggagagatcg atcgccgccc gccatgcggt gacgccaagc
    46861 tgcggctgac tgcgctgctt atgcatcggt actgacattt cttcgttatc gtgttctgta
    46921 cgcagcataa tgcgctgcgg tgggtcttcg ctcgtcagcc acagtgtaat gagcagttga
    46981 tctttgcttt gtttaacaaa tgggggcacc ggcagatgcc atgcatttaa catcataatt
    47041 cccctgtgat gaaaatctga tcaccttgcc atagcctaat gcaggatcac tcatcatctg
    47101 aaggatattt gggggaggag gacggaggcg gataactttc ggaatagccg gatcaggatt
    47161 tcctcctgaa ccggcttaca gaacgcaagg attactggtt ttcagccagc tgacgatcgt
    47221 ggcggcgctt aaacatccag ccaatcaaca gcacaacaat ccacgcgaaa ccgacataca
    47281 gcgagatacg cgtatcaggg tgataaccaa tcaacccgat aataaagagc aggaaaatca
    47341 aaccgccgat ggtcgttgct accccacccg gcactttaaa tttcagcgcc ttaacttctt
    47401 ctggcggcaa acggcgacgg aaggcgattt gcgacagcag gatcataatc cacacccaca
    47461 ccgtggcgaa ggttgccagc gaagcgatca ccaggaagac gttttccggc atgatgtagt
    47521 tcagatacac cgcaaacagc agcgcggtag tcatcaccag caccgtaacc cacggaatac
    47581 cgcgacgcga ggttttgctg aaaatttttg gcgcgctgcc ctgctctgcc ataccgtgga
    47641 gcatacggcc tacaccaaat acgtcactgt taattgccga cagcgaagcg gtcagcacaa
    47701 caaagttaag aatgctggcg gcaaaggtaa tgcccatatg ctggaacgtt agcacaaacg
    47761 ggctaccggc agtgccaacc tgattccacg ggtagataga cataatgacg aacagcgtac
    47821 cgacgtagaa caccagaata cgcatcggca cggagttaat cgcacgcgga atcgatttct
    47881 caggatcttt cgcttcaccg gcggtaatcc cgataatttc gatcccaccg taagcaaaca
    47941 tcaccatttg caacgacatc accataccaa gccagccgtt actgaagaag ccgccgttgc
    48001 tccacaggtt atgaataccg gtcggttgcc cgccgttgcc aatcccccag atgatgatgc
    48061 cgaaaccggc gacaatcatg atgatgatag tggcgacttt aaagaacgag aaccagaact
    48121 ccagctcacc gaataccttc acgctcatca ggtttacggc gcaaatgatc agcaccacac
    48181 tcagtaccca aatccagtgc ggcaccgtcg ggaaccagac acccatatag ataccaaaag
    48241 cggtcacatc ggcgatggcg acaataagga tttcaaagca gtaggtccag ccggtaatgt
    48301 aacctgccag cgggccgagg ttttcctgcg cataacgcga gaaagagctg gctgccgggt
    48361 tatgtaccga catttccccc agcgcacgca taatgatata cgccgcgata ccaccgataa
    48421 tataggccaa caacacgctt ggaccggcca ttttgatggc atctgccgaa ccgtaaaaca
    48481 gcccggtgcc aattgctgaa cccagtgcca taaagcgtat gtgtcgggta cttagcccac
    48541 gctttagctt gttcttactt tccatcaatt cctaacccat caacacaata aaaaaccacg
    48601 gggcatcgca gccccgtggt taaaacaaat gttcagtgat ttagtgagcg ctggaggtca
    48661 cctgacgacc tgccgcgcga tcccagataa tggccagaac caccatcacc actgttggca
    48721 ttaaccacgc cagaccttgt tcggccagcg gtaaacgctg cgcccaggac ggtaagatat
    48781 cgctgaatgc agatgctttg atcccgtcga gaataccaaa aagcaggctg ataaacatcg
    48841 gcggagcaat cacgcgggac gaattatgcc accatgagcg ggtaaaactt aatacaacca
    48901 gtgcgataca cggcggataa atggctgtca gcaccggtac agagatctga atcagctggc
    48961 tcaagccgag gttagacacc accatcgaga agccgccgag gataaacacc agcgtacgat
    49021 aagagagcgg tacgtactgg gcgaagaatt ctgcacaagc acaggtcagg ccaaccgccg
    49081 tgaccaggca ggcgatgaag attaacgccg ccagcaggaa gctaccgccg ccgccaaagg
    49141 tatgctgaac gtaagcatgc aggatcgccg caccgtttgc agactgatcg accagcgacg
    49201 cgctgtctga acccagacgg aacagcgcca ggtacagcag agtcagacca acacccgcca
    49261 tcaggccagc ccagacggta taacgggtca gcagacgcgc ttcggtaacg ccacgagaac
    49321 gcgccgcgtt aacaataacg ataccaaaca ccattgcgcc cagcgtatcc atggtcagat
    49381 agccgttgac gaagccgtta gaaaacgcag cgttttgata agcctcagtc gccgtgctga
    49441 tagaacccgc cggccagata attgcggcaa cagacaggat gaccagcgcg ataattttca
    49501 gcggcgcaag gaagttgccc acggtatcca gcagcttgcc cggatagagc gaaaccagaa
    49561 taacgatagc gaaatagacc aggctgtaaa taaacagcgg cagcgcggaa tcacccgtca
    49621 gcggcgcaat gcccacttca aaagaaacgg tagctgtacg cggcgtagcg aaaagcggcc
    49681 ccaccgccag gtaacaaact gttgccagca gtacgccagc gactttacca attggcgtgc
    49741 tgagactgtc aacaccgccg ccaacttttg ccagcgccac taccgttaat accggtaggc
    49801 caacggcagt aatgaggaag ccgaatgccg cagtccagac gtgttcgcct gcctgcaagc
    49861 cgaccattgg agggaaaata atgttacctg cgccgacgaa caacgcaaat gtcataaagc
    49921 ccagagcgat gatatcgcgc gatcttaatt gatgggtcat aaatcatact gcctgtggat
    49981 gtggtgttgt aacgttaaat ttttgtgcca atccctgggg atgatacagc ggtatattta
    50041 aacaatttta gcgggaaata ctcccaacca ggcgcggcga agaatagttt ttcgatttac
    50101 cgcgtaaatg cagtcagtat gacagcacct gaagcgcaat ttaaacgctt ataacgtttt
    50161 aaagcaagaa gggatctaaa aaccagaggt atatagcgat aagaaatcac caaccagtat
    50221 aaaacactat ttcaatgaaa aagacatggc taatcatgcg aacaaaaaat caccaaccat
    50281 tgtaaaatct aacgcccgta tttcgactgc aaagaaaata agccagcatc ggctggctta
    50341 tggcaataaa agatgacaaa ggcgaattaa tcgctgtttt tggcaattaa acgttccggg
    50401 ataacaaaac tgaaacgtgt accttttcct actgtactct caatattcag gcgactttcg
    50461 tgatgattca ccgcatgttt cacgatcgct aaccctaatc cgctaccacc ggtttgccgg
    50521 gaacgcgctt tatcaacgcg ataaaaacgc tcggtcaggc gcggaatatg ctccggtgca
    50581 atgcccggtc cgttatcttc aacactaaat tcggcaccgt gcggcactcg ctgccagcgt
    50641 acggtgatat gcgtgccttc cggcgtatga ttcacggcgt tatagaccag gttcgaaatc
    50701 gcactgcgta gctgatcttc attgcctgac accttgaggc cgttatctat ctcaaaggta
    50761 aatgtctgtt ttttctgact cagagtctga gcctcgcgct caacgacgcg cagcatcatc
    50821 ggcacatcaa ccttttcatt gagcaaatgc gtcggtgcgg cttctatttt cgacagcgtc
    50881 agcaattgct tcaccaaccc ttccatccgc tgggtctgct cgcgcatggt gtgcaacgct
    50941 ttttcacgta ccgcgccttc cagcggttgc tcatccatca tctccaggta accctgtaac
    51001 acggtcaatg gcgtacgtaa ctcatggctc acgttggcaa aaaagttacg ccgcgcccct
    51061 tccagttgat gcatttgcgt gacatctcgc gccaccatca gcaactgttt gtgggtataa
    51121 ggcatgacgc gaatctccag atgccgcccg gtgttgagca ccagattgag cgggcgagag
    51181 aaatcacgcg ttttcagata ttgcgtaaac tccgggtaac gcagcaagtt aaggatgttc
    51241 tgcccgttat cttccggcca gcgcaagcca agaatctgtt gcgccagacc gttgcaccag
    51301 aaaataccgc cctcttccgt ggtcagcacc accgcgtcgg gcagcgactc cgcgccgcta
    51361 cgaaagcgtt taatcagatt gcccagttca cggcggcgtt ttttatttcg cagctgcatc
    51421 tggtgtaagc cgtatagtag cggttcccag ctaccacgcc ccggtggcgg ggtcatacta
    51481 cgatccaccc acagccacca tgaaaggcgc aataaattcc agaaatgcca gataagcagt
    51541 cctgttaccg atgccagcaa aaaccagggc aggtaaccaa aaaatgcacc caggatgaaa
    51601 gccaggaggc agcaaagtag cagctccagc accagccttt tccacgacag ccgttccagc
    51661 acgtaagata ctccagttaa gaaatcataa gccctgctct acgtccgatg aacaaggcct
    51721 taaaagcggg ttgaaaaacg ataccctgta ccgcgcacgg tctgcaccat gcggtcatgc
    51781 ccgccgggct ccagtgcttt acgcaggcga cgaatgtgga catcgaccgt acgatcttcc
    51841 acatacacgt tggttcccca gacgtggttt aacagctgct cgcggctgta cacgcgctca
    51901 ggatgcgtca taaaaaagtg cagcagttta aattctgtcg gccccatctc cagcggttct
    51961 tcgcccgcca tcacccggtg agatgtcggg tccagactta gcccctgcat ctcgatcacc
    52021 tcttccaccg ccattggcga aatacggcgc attaccgctt tgattcgcgc caccagctcc
    52081 ttcggagaaa acggcttggt gatatagtca tccgcaccgg tttcaaggcc gcgcacgcga
    52141 tcttcttctt cccctctggc ggtcaacatt accactggga tatcccgggt catcgactcg
    52201 cgcttgaggt gtttgatgaa ctggataccg gagccgccag gtaacatcca gtcaaggaga
    52261 attaaatccg gccagggttc attcagttga ttcacagcac tgtcataatc ttccgcttcg
    52321 accggctgaa agccattttg ttcgagcacg aagcagacca tttcgcgaat tggagcttca
    52381 tcttctacga ccagaatacg tctcgccatg atttgccctg ttgtaataga taggttgcga
    52441 tcattaatgc gacgtcatta tgcgtcagat ttatgacaga tttatgaaaa gctcgtcgca
    52501 catatcttca ggttattgat ttccgtggca cagaaaaaag caaatggcac atctgtttgg
    52561 gtataatcgc gcccatgctt tttcgccagg gaaccgttat gcgcatcctt cacacctcag
    52621 actggcatct cggccagaac ttctacagta aaagccgcga agctgaacat caggcttttc
    52681 ttgactggct gctggagaca gcacaagccc atcaggtgga tgcgattatt gttgccggtg
    52741 atgttttcga taccggctcg ccgcccagtt acgcccgtac tttatataac cgttttgttg
    52801 tcaatttaca gcaaactggc tgtcatctgg tggtactggc aggaaaccat gactcggtcg
    52861 ccacgctgaa tgaatcccgc gatatcatgg cgttcctcaa tactaccgtg gtcgccagcg
    52921 tcggacatac gccgcaaatc ttgcctcgtc gcgacgggac gccaggcgca gtgctgtgtc
    52981 ccataccttt tttacgcccg cgtgacatta ttaccagcca ggcggggctt aacggtattg
    53041 aaaaacagca gcatttgctg gcggctatta ctgattatta tcaacaacac tatgccgatg
    53101 cctgcaaact gcgcggcgat cagcctctgc ccatcatcgc cactggacat ttaacgaccg
    53161 tgggtgccag taaaagtgac gccgtgcgtg acatttatat tggcacgctg gacgcgtttc
    53221 cggcacaaaa ctttccacca gccgactaca tcgcgctcgg gcatattcac cgcgcgcaga
    53281 ttattggcgg catggaacat gttcgctatt gcggttcccc cattccactg agttttgatg
    53341 aatgcggtaa gagtaaatat gtccatctgg tgacattttc aaacggcaaa ttagagagcg
    53401 tggaaaacct gaacgtaccg gtaacgcaac ccatggcggt gctgaaaggc gatctggcgt
    53461 cgattaccgc acagctggaa cagtggcgcg atatatcaca ggagccgcct gtctggctgg
    53521 atatcgaaat cactactgat gagtatctgc atgatattca gcgcaaaatc caggcattaa
    53581 ccgaatcatt gcctgtcgaa gtattgctgg tacgccggag tcgtgaacag cgcgagcgtg
    53641 tgttagccag ccaacagcgt gaaaccctca gcgaactcag cgtcgaagag gtgttcaatc
    53701 gccgtctggc actggaagaa ctggatgaat cacagcagca acgtctgcag catcttttcg
    53761 ccacgacgtt gcatagcctc gccggagaac acgaagcatg aaaattctca gcctgcgcct
    53821 gaaaaacctg aactcattaa aaggcgaatg gaagattgat ttcacccgcg agccgttcgc
    53881 cagcaacggg ctgtttgcta ttaccggccc aaccggtgcg gggaaaacca ccctgctgga
    53941 cgccatttgt ctggcgctgt atcacgaaac cccgcgcctc tctaacgttt cacaatcgca
    54001 aaatgatctc atgacccgcg ataccgccga atgtctggcg gaagtggagt ttgaagtcaa
    54061 aggtgaagcg tatcgcgcgt tctggagcca gaatcgggcg cgtaaccagc cggacggtaa
    54121 tttgcaggtg ccacgcgtag agctggcgcg ctgtgcagac ggcaaaattc tcgccgacaa
    54181 agtgaaagat aagctggaac tgacggcgac gttaaccgga ctggattacg ggcgcttcac
    54241 ccgttcgatg ctgctttcgc aggggcaatt tgctgccttc ctgaatgcca aacccaaaga
    54301 acgcgcagaa ttgctcgagg agttaaccgg cactgaaatc tacgggcaaa tctcggcgat
    54361 ggtttttgag cagcacaaat cggcccgcac agagctggag aagctgcaag cgcaggccag
    54421 cggcgtcgcg ttgctcacgc cagaacaagt gcaatcgctg acagcgagtt tgcaggtact
    54481 tactgacgaa gaaaaacagt taattaccgc gcagcagcaa gaacaacaat cgctaaactg
    54541 gttaacgcgt ctggacgaat tgcagcaaga aggcagccgc cgtcaacagg ccttgcaaca
    54601 ggcgttagcc gaagaagaaa aagcgcaacc tcaactggcg gcgctcagtc tggcacaacc
    54661 ggcacgaaat cttcgtccgc actgggaacg catcgcagaa tacagcacgg cgctggcgca
    54721 tactcgccag cagattgaag aagtaaatac tcgcttacag agcacaatgg cgcttcgtgc
    54781 gagcattcgc caccacgcgg cgaagcagtc agcagaatta cagcagcagc aacaaagcct
    54841 gaatgcctgg ttacaggaac acgaccgctt ccgtcagtgg aacaacgaac tggcgggttg
    54901 gcgtgcgcag ttctcccaac aaacctgcga tcgcgagcat ctgcggcaat ggcagcaaca
    54961 gttaactcat gctgagcaaa aacttaatgc gcttgcggcg atcacgttga cgttaaccgc
    55021 cgatgaagtt gctaccgccc tggcacaaca tgctgagcaa cgcccactgc gtcagcgcct
    55081 ggtcgcactg catgggcaga ttgttcccca acaaaaacgt ctggcgcagt tacaggtcgc
    55141 tatccagaat gtcactctgg aacagacgca acgtaatgcc gcacttaacg aaatgcgcca
    55201 tcgttataaa gaaaaaacgc aacaacttgc cgatgtgaaa accatttgcg agcaggaagc
    55261 gcgcattaaa acgctggaag cccagcgcgc acagttacag gcgggtcagc cttgtccact
    55321 ttgtggttcc agcagccacc cggcggtcga ggcgtatcag gcgctggaac cgggcgttaa
    55381 tcaggcccgg ctattaacgc tggaaaaaga agtgaaaaag ctcggcgaag aaggtgcggc
    55441 gctacgtggg caactggatg cattaacgaa gcagcttcag cgcgatgaaa acgaagcgca
    55501 aagcctccgg caagatgagc aagcacttac tcaacaatgg caagccgtca cggccagcct
    55561 caatatcacc ctgcagccac aggacgatat tcaaccgtgg ctagatgcac aagatgagca
    55621 tgaacgccag ctgcggttac tcaaccaacg gcatgaatta caagggcaga ttgccgcgca
    55681 taaccagcaa attatccagt atcaacagca aattgaacaa cgccagcagc aactcttaac
    55741 ggcattggcg ggttatgcac tgacattgcc acaggaagat gaagaagaga gctggttggc
    55801 gacgcgtcag caagaagcgc agagctggca gcaacgccag aacgaattaa ccgcgctgca
    55861 aaaccgtatg cagcagctga cgccgattct ggaaacgttg ccgcaaagtg atgaactccc
    55921 gcacagcgaa gaaactgtgg cgctggaaaa ctggcggcag gttcatgaac aatgtctcgc
    55981 attacacagc cagcagcaga cgttacagca acaggatgtt ctggcggcgc aaagtctgca
    56041 aaaagcccag gcgcagtttt acaccgcgct acaggcgagc gtctttgacg atcagcaggc
    56101 gttccttgcg gcgctcatgg atgaacaaac actaacgcag ctggaacagc tcaagcagaa
    56161 tctggaaaac cagcgccgtc aggcgcaaac gctggtcact cagacagcag aaacgctgac
    56221 acagcatcag caacaccgac ctggcgggtt gtctctcact gtgacggtgg agcagattca
    56281 gcaagagtta gcgcaaactc accaaaagtt gcgtgaaaac accacgagtc aaggcgagat
    56341 tcgccagcaa ctgaagcagg atgcagataa ccgtcagcaa caacaaacct taatgcagca
    56401 aattgctcaa atgacgcagc aggttgagga ctggggatat ctgaattcgc taataggttc
    56461 caaagagggc gataaattcc gcaagtttgc ccaagggctg acgctggata atttagtcca
    56521 tctcgctaat cagcaactta cccggctgca cgggcgctat ctgttacagc gcaaagccag
    56581 cgaggcgctg gaagttgagg ttgttgatac ctggcaggct gatgcggtac gcgatacccg
    56641 taccctttcc ggcggcgaaa gtttcctcgt cagtctggcg ctggcactgg cgctttcaga
    56701 tctggtcagc cataaaacgc gtattgactc gctgttcctt gatgaaggtt ttggcacact
    56761 ggatagcgaa acgctggata ccgcccttga tgcgctggat gccctgaatg ccagtggcaa
    56821 aaccatcggt gtgattagcc acgttgaggc gatgaaagag cgtattccgg tgcagatcaa
    56881 agtgaagaag atcaatggac tgggctacag caaactggaa agtgcgtttg cagtgaaata
    56941 actattcagc aggataatga atacagaggg gcgaattatc tcttggcccg actggtcgtt
    57001 atcctgcaag ctatcacttt attggctacg gtgattggta gccgttctgg tggttgtgat
    57061 ggtggtatga aaaaagttat tttatctttg gctctgggca cgtttggttt ggggatggcc
    57121 gaatttggca ttatgggcgt gctcacggag ctggcgcata acgtaggaat ttcgattcct
    57181 gccgccgggc atatgatctc gtattatgcg ctgggggtgg tggtcggtgc gcctataatc
    57241 gcgctttttt ccagtcgcta ctcactcaaa catatcttgt tgtttctggt ggcgttgtgc
    57301 gtcattggca acgccatgtt cacgctctct tcgtcttacc tgatgctcgc cattggtcgg
    57361 ctggtttccg gctttccgca tggtgcattt tttggcgtcg gcgcgatcgt gttatcaaaa
    57421 attatcaaac ccggcaaagt caccgccgcc gtggcaggga tggtttccgg gatgacagtc
    57481 gccaatttgc tgggcattcc actgggaacg tatttaagtc aggaatttag ttggcgttac
    57541 acctttttgt tgatcgctgt ttttaatatt gcggtgatgg catcggtcta tttttgggtg
    57601 ccggatattc gcgacgaggc gaaaggaaaa ctacgcgaac aatttcattt tttacgcagc
    57661 cctgccccgt ggttaatttt cgccgctacg atgtttggca acgcaggtgt gtttgcctgg
    57721 ttcagctacg taaagccata catgatgttt atttccggtt tttcggaaac ggcgatgacc
    57781 tttattatga tgttagttgg gctagggatg gtgctgggga atatgctaag tggcaggatt
    57841 tcaggacgtt attcaccact gcgcattgca gcagtgactg actttatcat tgtactggca
    57901 ctgctgatgc tctttttctt cggcggtatg aaaacaacgt cgcttatttt tgcttttatt
    57961 tgttgcgcgg gattatttgc cctatcagca cctctacaaa tattattact gcaaaatgcc
    58021 aaaggcggag agttattagg tgccgcaggt gggcaaatag cgtttaacct cggtagcgcc
    58081 gtcggcgcat attgcggtgg tatgatgctg acgctggggc tggcatataa ttacgtggcg
    58141 ctgcccgctg ccctgctttc gtttgctgcg atgtcgtcgt tgctgctgta tggtcgctat
    58201 aagcgccagc aagcggcgga tagttcggtg ctggcgaaac cactggggta gatagcagtc
    58261 gcagtgcgca tcgcttgttg ccggatgcgg cgtgaacgcc ttatccggcc tacgggactg
    58321 ttcattttgt aggcctgata agacacgcca gtgtcgcatc aggcatcgga gcactggggg
    58381 gttgccgcca ctgtgcgcgt tatttgttgt cggatgcggc gcgagcgcct tatccggcct
    58441 accggacggc tcactttgta ggcctgataa gacgcggcaa gcgtcgcatc aggcatgata
    58501 cgccaattgc ctggtttttt tactcttgtg gccataacca tgcagcgccg cgtacgccgc
    58561 tcgaatcacc atgtttcgcc ttgcgtaccg gcgtttcaca ttctccgccg aagacaaatt
    58621 gtttaatcaa ctgtggaacc gtttgatata aacggtctac attgctcatt ccgccgccca
    58681 ggacaatcac atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca
    58741 gctcgtagcg acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggcggataa
    58801 tttcactgcc tttcagcgca tgtccgctca aacgacgata atccgtcgcg aatcccgtgc
    58861 ctgaaataaa ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat
    58921 aacgcagttc gtcttcgtcc atccacggta gcggattgtg tccccactcg cctgccgtgc
    58981 cattgccgcc gatatgcgcc cgcccattga atgccacgcc cgcgccgcat cccgtgccga
    59041 taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc atctaccgct tctgaaaccg
    59101 ccagacagtt ggcgtcattt gccagccgca cttcccgctg caaccgcgcg cttaagtctt
    59161 tatcgaatgg ctgaccgttg agccaggttg aattggcatt cttcaccacg ccggtgtagg
    59221 gcgaaatcga accaggaatg cccataccta ccgttccgcg ctgccctgtt gcctgctccg
    59281 ccatatcaac caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg
    59341 gcagacgatg gcggtacaac tgctcccctg catcgcccaa tgcaatcact tcagttttgg
    59401 tgccgcctaa atcgatacct atacgcacgg tactctcctt atttttttca atatcaatag
    59461 cgtagagacg gacaaccgga ttggcaatgc aaggccgccg acaattcgtt atcatgcccg
    59521 ctaaatttaa cgacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt
    59581 accgtcttag ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga
    59641 tggcatttac cccatgcggc agccaggaca tggcgaagat gggctgggta cctccgatgg
    59701 gatcgcacag cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgtgcgcgca
    59761 aagaagaaaa aatcctcccg tccccggtga ttaaacaggc gctggaagcg aaaatcgcca
    59821 aactggaagc ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg
    59881 aagtgctgca ctctctgctg ccgcgtgctt tcagccgttt tagccagaca atgatgtgga
    59941 tcgacacggt taacggtttg attatggtgg actgcgccag cgccaaaaaa gcggaagata
    60001 cgctggcact gctgcgtaaa agcctggggt cgttaccggt tgtaccattg agcatggaaa
    60061 acccgattga actgacgctg accgaatggg tacgttccgg cagcacggca cagggcttcc
    60121 agctgcttga tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga
    60181 agaaacaaga tctgaccagc gaagagatca ccaaccacat tgaagccgga aaagtggtga
    60241 ctaaactggc gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc
    60301 tcaagcgtct gaagttctgc gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag
    60361 atttcgccca gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa
    60421 ttcaaaacct gattgaagga ttaggtggcg aagcacaacg ttaattgctg atttaccttt
    60481 aatgccggat gcgacgcctg ccgcgtctta tccggcgtac gaagccacac caggaatata
    60541 atcgttagct acagcgggca ataattctta gcgcagcaat attatgcgtt ttatgctgta
    60601 acttgctcca ggggcgttgt gtcattgttt ttcctcaagc cggtgacgcg cttcactgcg
    60661 aattttccgc gttgccccgt ttttcactaa atcacgtaac agcgaaagat cgggttgttc
    60721 gacaaataag agtaatgatg gctcctcttt taaccacgcc gttttcacat cggcagccag
    60781 ctgtgaatta ttaatagcca gcgaccgctc ctgaggctca gtcaatgtcg ttaacaacaa
    60841 caccggggta tgtgcgttgt gcaatgccgt ttggcgcaaa tgatcattgc gactttgcag
    60901 caatgcacgc caccattcat catctgattt atcttttatc gtcgccagtt ccgcaacaat
    60961 atctggagat cttcgctgat atttatatca accttgatga attatgaata tatcggaaat
    61021 cgaaaaaatc ccagcaaagg aaatattatt atgggttaaa gagaggacat ttcagaaaaa
    61081 tgccctcccg ttcaagggga aggcgaggaa ttacagatag cggcacagat aagaggtggg
    61141 ttcggcaact tgcagatgaa actcgctgtg accgggaaca ttaaacaccg aaccggcttc
    61201 atacacttgc cagtcggtcg cgtcaggcag taacacattc agcgcgccac tgattaccgt
    61261 catctcttca ggctcagcgg tgctgaaggt gtattcacct tcaaccataa cacccacgct
    61321 ggcgcgacca gtgctgctgc tggaaaagcc gatcgatttc actttgccgg aaaagtactc
    61381 attactttga agcataaact ggccctgtct ggtagttgaa catctacttc actatagggg
    61441 ccagaggcgc tggctgtcac gcaaaattac atcagtaatt ccgcagccag ccgtgcaatc
    61501 aatacgttag agagcaagac aggaacatcg agctgttttt gcagtaaatc gcgatgacgc
    61561 tggttaaatc ccaaacaatc cagcatgatg acatctgcac cttttgccag taattctttc
    61621 ccggcatcaa tgattttttg ttccgaatca tgaatggggt tacccaatga aaatactggc
    61681 ggtttctgca aaatttgcca tttttgcgcc tgaacggtca gcaactcctc aaccggtacg
    61741 ataaccccca cctgatgatc ttcaacaata gagaaaacca gcggtggcaa tatccgcgac
    61801 ggctcaagaa agatcgtatt acgcgcggtc atactactaa tgtttgctgt actcattaat
    61861 ataatgacgt cgtaaccctg attatcgagc acttcaacca caccttgcag gtctcgctcc
    61921 actttgcgac gcgaaacatg ggccagatgg ttgtcattta ataatgtgag aatggtgtct
    61981 tcgcctgctt ctggcgcgta ctccgccatc acttcttcgc ggcttaactt ccccagcagg
    62041 ctatgatggg aaatattatc ttcgtcaatg tattctgtca ggagcggcaa aacttcctgc
    62101 ataggtacaa tgccgatagt caggatcgcc aacgacgcac tcattgttct tccaccttac
    62161 ttttatttcg tcgttaatta ccgggcaagt gttaagcacc actgctgaca cttacttgtg
    62221 ctacaaatga caaaaagcgt agcagcagac ctgcactgca taatgtaaag attccaaatg
    62281 attccagtaa tggattgtta ttgtttaata ttctaattat ttgaaaaaca tgaattgtga
    62341 aaaatgtgac gcagatccct tgtggcggca ctcagaatcc cttccgagca gtctgatgcg
    62401 gtcactcttt gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc tgagcggtcg
    62461 gatgttcact gatcaacgag tctggtttag gatgatcggc tctgagttgg taccaggtta
    62521 ccgtctgtcc tggctttcct ttatcaatcg tcactatata tgcttcacga ggataaggcg
    62581 gtttcgttgg catcgttctt ccttatttca cgggatgaac gttaagtata ggcgctcgaa
    62641 aatcaacaat tgatcgtctg tgccagagcg ctgcgaattt cagaaatcac ctggctgggt
    62701 tcgtttgttg cgtcgatgat aatatgcgca acttcgcgat atagcgcatc gcgttcttcc
    62761 agcacttcct gaacttcttc gctcagtggt tttcccgtta aggttggccg taaatcttct
    62821 tccggtgcag cttgcagtcg gttaaccagg actgatactg gcgcacacaa ataaaccacg
    62881 atcccgttat tttgcatgaa gtgacgatta aattccgtca gaataatgcc gccgcctgta
    62941 gcgataacgg tggatggcgc agttaccgct tccagcgccg ccgtttctct ggcgcggaat
    63001 cccgcccact cttccctttc gacgatctcc gcgaccgtca tattgagctg tgattgcaac
    63061 cacaggtcgg tatcgacaaa ccgacggtta agcgaatcgg caagggccat tccgaccgtt
    63121 gttttaccac agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc
    63181 caataggtcg caatgcggcg aaagccggtg tcatgagaat agcgatcata ccatcaaact
    63241 agtacaattt cgattgtaaa gaaaaaattc cacttaaggt ggaaatctca atacatccct
    63301 tactatacca ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac
    63361 tacgttgcac ttccagccac cacttctcca gctccgcagc aaaagcctgg cggtcacgtt
    63421 gtgaaagact atctggtcct ccggtctgga tcccactggc acgtaaggta tccataaaat
    63481 cgcgcatcgt caggcgctca cgaatggtcg ctggcgtgta acgttcgccg cgcggattaa
    63541 gcgccgccgc gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca
    63601 aatcgcccgc ttcacactgc cgaacaattt cgttatcagc aacgtcgaaa cctgccgcga
    63661 cgcgcagcgt acgaataaat cgtgatggcg gcacgcgtaa actctggttt gctaccagta
    63721 ccagcggcat ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac
    63781 acgcgtcggc atccacccaa attgtcataa agtcatcctt tgttgggtaa tcctctattg
    63841 tgtcgcgctt ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac
    63901 aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc
    63961 aatatgggaa aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa
    64021 atctgggtat ataccccctc cccggataaa gtcaccgccc tgcatgacca gttcggcatc
    64081 aacgccgcag aatcggcgca agaagtagcg caaatcgccg acatcatttt tgccgccgtt
    64141 aaaccaggca tcatgattaa agtgcttagc gaaatcacct ccagcctgaa taaagactct
    64201 ctggtcgttt ctattgctgc aggtatcacg ctcgaccagc ttgcccgcgc gctgggccat
    64261 gaccggaaaa ttatccgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc
    64321 tccgtaacgc caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc
    64381 cgctgctttg gcgaagcgga agtgattgct gagccgatga tccacccggt ggtcggtgtg
    64441 agcggttctt cgccagccta cgtctttatg tttatcgaag cgatggccga cgccgccgtg
    64501 ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt aatgggctcc
    64561 gcaaaaatgg tgctggaaac gggagaacat ccgggaacac tgaaagatat ggtctgctca
    64621 ccgggaggca ccaccattga ggcggtacgc gtactggaag agaaaggctt ccgtgccgca
    64681 gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatgg
    64741 cttttgccgg acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt
    64801 agagcgccaa atctgccgat ttcaaccact cgcgatagtg actcatttgt ggattcagcg
    64861 gcgcaacccc cacactaatc cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt
    64921 ttagcccttc atgcacccgt aacatggcgg tgatggcgct ctcagctggc gtaccggaca
    64981 tgattactgc aaactcatcg ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa
    65041 tttgtaactg tcgggtaagc gccacaatcg cttcatcacc cacatcatgg ccccaggtat
    65101 cgttgatgct cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg cgattatgcc
    65161 gccgacagtt atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc
    65221 ccgtcatacc gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc gccagtttag
    65281 tcgccgtctg gtagctgacc cagccaaaca gcagaggata aatgacaata atgggaaggg
    65341 agagccacca ttccagcggt gcgctattga acgacacggt gatgcccgtc agctcgaggg
    65401 tgacaaggca ggagaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg
    65461 cccccatcaa attcagacac ataatcatca acatcgcggt ggaaggcagt acgtttacgc
    65521 ccattacgcc tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc
    65581 ggctaagcgg atcgacggcc ctgctcgcta tttgccaggc taaatgcggc cagacgaacg
    65641 cccagccgac caacaacagc caccaccagc ccggcgacgg atgcgaaacc agcgttgaag
    65701 caatcggtaa gaacatgcca gccaggccaa ccgcgcgggg tagtctgacg cgacgggcaa
    65761 agcgcagccc ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccatgcg
    65821 ccgccgcttt tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat
    65881 tttcccaaat tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccattt
    65941 tcgtagctca cccgttagca ctatcaggta tcaggcggct ttcttgaggc agacactcat
    66001 aaacttatta cgatcatccc ctttcagaga ttgttgtgtt gcttgattat tacattcgcg
    66061 catcttttgc tgctgtggcg tcaaactttt ttcaccaggc gcagatttgc tgttcttcag
    66121 gcaatcactc atgtaggtct tacgagcatc ccctttcaac gcctgcgccg tcgcctgctg
    66181 attacaagag gtcatacgct gttgttgtgg ggttaaggtt ctctcggcag cgccgacggt
    66241 ggttaaaaaa accagaccga aaagcaaggt aaccagtaat gtaattttca tcgcaccatc
    66301 cctcttcatg ttttaaccat gagcgtatgc gcccgtgatc tgtcattaag tctggttgct
    66361 aacagcaaaa aaaccacccg gcagcgaaaa ttcactgccg ggcgtggttt tatttcagcc
    66421 ccagggcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca acaacattgg
    66481 cggcatgagg gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct
    66541 cttcggagtt cccgtaactc atcaccatca ctgcgccatc tttggtattt agcgcctggg
    66601 tgaggcccgg agctttggta tccggcgcaa ctatctggct ggcatgggcg tgatcagcgg
    66661 tgactatgac caacgtgtta ccatcctttt tagcgaattc cagcgcccgt tgtacggctt
    66721 catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga tcctgtttat
    66781 cgattgacgc accttcaact tgcaggaaaa agcctttctc atttttactc aacaattcaa
    66841 tggctttgtc ggtcatctgc gccagggtcg gtacactgtc attacgttgc ggattaggcg
    66901 tacaggtgac tgcgggctta tctatattac cgtggtacgc tgctttcggt ccttgccagc
    66961 gcactggcat attgccgtca gcaaacagtc ctaatagggg tttttgctga ttcgcttccg
    67021 tcaccgaatt cagtgaggca gcatcgctca ccaactgata accacgcgcc tgtgcctgtt
    67081 cacgcagcgt ttttccctgc cattcaccgg cggttgccgt ttcagcaaag gtttttgcgc
    67141 cgccgccaag cgtaacatcg gcacgggcgt taagcagctg ttcggtaatc gatccttttc
    67201 cgcctttttc cagagcgtta cccggacatt tttcactggt cgcactcgga ccgtagcatt
    67261 tgcgcgaggt cacatgcgcc accagcgcag cgggcgtggc atcctgcaac tctgcggtag
    67321 aaacgttacc ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt
    67381 tttcgtgaat atcgacgccc agcgcgccgt tataggtttt gacaccagtt gaccaggcgg
    67441 ttgctgatgc agccgagtcg gtgacgtagt ccggtttgcc ggttttttta ttcagcgcat
    67501 agtgagtgta ttgcccggta agcggtaagg catcgatacc tttaaaaaag ccgcccgcac
    67561 cttcggcata attgcgtgcg gcagtaattt ccgagtcccc catcccatcg ccaatcagca
    67621 aaataatatt ttttgcaggt ttatcgctaa gagaatcacg cagagcggcg gtctgatcac
    67681 ccgttaagcg gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc cggttttcca
    67741 gaacaggcat ttctggtgtc cgggcttttg tcacaggggt aaacagtaac agtaagagtg
    67801 ccagtgcaat agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaaata
    67861 aaaacaaagc gactataagt ctcggccgtg acaactttat gacagctgtt gaaaagatta
    67921 actttttact gacgaggatg cctcaataac ttctttacgt aatcgcgcag cagctccgta
    67981 tcgtcgtcag gaatgctggc atcgggcttt acctcgtaca gcgccccctc tacctgatca
    68041 atcaaccgct gttgatcatt ttgcgccata ttgcgaagca ttgccgtgac gataatctcc
    68101 aaagcttcta cctgcgcaca cagttctttc gactcttctt ctttttgggc aagcttaaat
    68161 aacaactcag caatgagatt tttcatgtat gtatttcctt atccaaagta tggagaagtt
    68221 aacattatgt ttcacaactt catagtgtct ggaagtatta tttttatgac aaaggaaata
    68281 ctttgcgcaa atattttttt gtttgattac cagcgaaacg tt
//
LOCUS       DAZGJT010000009        67811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZGJT010000009 DAZGJT010000000
VERSION     DAZGJT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.009"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..924
                     /locus_tag="ACHKFK_000652"
     CDS             <1..924
                     /locus_tag="ACHKFK_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HFO9051067.1"
                     /translation="PLIIFYQPWVNALPPTPRHASPEQLEKTVRYLTQTVHPRSADNI
                     DNLNKSAEYIKEAFISSGARVTAQDVPITGGPYKNIVANYGPADGPLIIIGAHYDSVS
                     SYENDQLTYTPGADDNASGVAGLLELARLLQQQTPGIGVQLVAYASEEPPFFRSDEMG
                     SAIHAASLERPVKLMIALEMIGYYDSTPGNQDYPSPAMSWLYPDRGDFIAVVGRMQDI
                     NAVRQVKAALLSSRDLSVYSMNAPGFIPGIDFSDHLNYWQHDIPAVMITDTAFYRNKQ
                     YHLPGDIADRLNYQKMAQVVDGVTTLLYNSK"
     gene            1027..1530
                     /locus_tag="ACHKFK_000653"
     CDS             1027..1530
                     /locus_tag="ACHKFK_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO9051068.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTKKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1597..3054)
                     /gene="nuoN"
                     /locus_tag="ACHKFK_000654"
     CDS             complement(1597..3054)
                     /gene="nuoN"
                     /locus_tag="ACHKFK_000654"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO9051069.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLVGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3061..4590)
                     /gene="nuoM"
                     /locus_tag="ACHKFK_000655"
     CDS             complement(3061..4590)
                     /gene="nuoM"
                     /locus_tag="ACHKFK_000655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO9051070.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALVTMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSNG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4754..6595)
                     /gene="nuoL"
                     /locus_tag="ACHKFK_000656"
     CDS             complement(4754..6595)
                     /gene="nuoL"
                     /locus_tag="ACHKFK_000656"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO9051071.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLVDAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSSPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6592..6894)
                     /gene="nuoK"
                     /locus_tag="ACHKFK_000657"
     CDS             complement(6592..6894)
                     /gene="nuoK"
                     /locus_tag="ACHKFK_000657"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO9051072.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6891..7445)
                     /gene="nuoJ"
                     /locus_tag="ACHKFK_000658"
     CDS             complement(6891..7445)
                     /gene="nuoJ"
                     /locus_tag="ACHKFK_000658"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO9051073.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7457..7999)
                     /gene="nuoI"
                     /locus_tag="ACHKFK_000659"
     CDS             complement(7457..7999)
                     /gene="nuoI"
                     /locus_tag="ACHKFK_000659"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO9051074.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8014..8991)
                     /gene="nuoH"
                     /locus_tag="ACHKFK_000660"
     CDS             complement(8014..8991)
                     /gene="nuoH"
                     /locus_tag="ACHKFK_000660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO9051075.1"
                     /translation="MSWISPELIEILLTVLKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRFGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8988..11714)
                     /gene="nuoG"
                     /locus_tag="ACHKFK_000661"
     CDS             complement(8988..11714)
                     /gene="nuoG"
                     /locus_tag="ACHKFK_000661"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO9051076.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFIILNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSAE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIIGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSQTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11767..13104)
                     /gene="nuoF"
                     /locus_tag="ACHKFK_000662"
     CDS             complement(11767..13104)
                     /gene="nuoF"
                     /locus_tag="ACHKFK_000662"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO9051077.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDSGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13101..13601)
                     /gene="nuoE"
                     /locus_tag="ACHKFK_000663"
     CDS             complement(13101..13601)
                     /gene="nuoE"
                     /locus_tag="ACHKFK_000663"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO9051078.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13604..15406)
                     /gene="nuoC"
                     /locus_tag="ACHKFK_000664"
     CDS             complement(13604..15406)
                     /gene="nuoC"
                     /locus_tag="ACHKFK_000664"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO9051079.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMVEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15500..16162)
                     /gene="nuoB"
                     /locus_tag="ACHKFK_000665"
     CDS             complement(15500..16162)
                     /gene="nuoB"
                     /locus_tag="ACHKFK_000665"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO9051080.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16178..16621)
                     /gene="nuoA"
                     /locus_tag="ACHKFK_000666"
     CDS             complement(16178..16621)
                     /gene="nuoA"
                     /locus_tag="ACHKFK_000666"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO9051081.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17251..18189)
                     /gene="lrhA"
                     /locus_tag="ACHKFK_000667"
     CDS             complement(17251..18189)
                     /gene="lrhA"
                     /locus_tag="ACHKFK_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO9051082.1"
                     /translation="MISANRLIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPST
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19109..20326
                     /gene="alaA"
                     /locus_tag="ACHKFK_000668"
     CDS             19109..20326
                     /gene="alaA"
                     /locus_tag="ACHKFK_000668"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO9051083.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20410..21009
                     /gene="yfbR"
                     /locus_tag="ACHKFK_000669"
     CDS             20410..21009
                     /gene="yfbR"
                     /locus_tag="ACHKFK_000669"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9051084.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21068..22900)
                     /locus_tag="ACHKFK_000670"
     CDS             complement(21068..22900)
                     /locus_tag="ACHKFK_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO9051085.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALVVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPI
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22987..23637)
                     /gene="hxpA"
                     /locus_tag="ACHKFK_000671"
     CDS             complement(22987..23637)
                     /gene="hxpA"
                     /locus_tag="ACHKFK_000671"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO9051086.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLSPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23648..24142)
                     /locus_tag="ACHKFK_000672"
     CDS             complement(23648..24142)
                     /locus_tag="ACHKFK_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO9051087.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24225..24680)
                     /gene="yfbV"
                     /locus_tag="ACHKFK_000673"
     CDS             complement(24225..24680)
                     /gene="yfbV"
                     /locus_tag="ACHKFK_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO9051088.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25018..26220
                     /gene="ackA"
                     /locus_tag="ACHKFK_000674"
     CDS             25018..26220
                     /gene="ackA"
                     /locus_tag="ACHKFK_000674"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO9051089.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26295..28439
                     /gene="pta"
                     /locus_tag="ACHKFK_000675"
     CDS             26295..28439
                     /gene="pta"
                     /locus_tag="ACHKFK_000675"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO9051090.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIIANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSTKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIDSLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRENYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28629..30149
                     /gene="yfcC"
                     /locus_tag="ACHKFK_000676"
     CDS             28629..30149
                     /gene="yfcC"
                     /locus_tag="ACHKFK_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO9051091.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAIFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30183..30725)
                     /gene="yfcD"
                     /locus_tag="ACHKFK_000677"
     CDS             complement(30183..30725)
                     /gene="yfcD"
                     /locus_tag="ACHKFK_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO9051092.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30783..31334)
                     /gene="yfcE"
                     /locus_tag="ACHKFK_000678"
     CDS             complement(30783..31334)
                     /gene="yfcE"
                     /locus_tag="ACHKFK_000678"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO9051093.1"
                     /translation="MKLMFASDIHGSLPATERVLELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31390..32034)
                     /gene="yfcF"
                     /locus_tag="ACHKFK_000679"
     CDS             complement(31390..32034)
                     /gene="yfcF"
                     /locus_tag="ACHKFK_000679"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO9051094.1"
                     /translation="MSKTAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPAWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32170..32817
                     /gene="yfcG"
                     /locus_tag="ACHKFK_000680"
     CDS             32170..32817
                     /gene="yfcG"
                     /locus_tag="ACHKFK_000680"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO9051095.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAGLDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAVTLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQHGDERSDS"
     gene            32874..33236
                     /gene="folX"
                     /locus_tag="ACHKFK_000681"
     CDS             32874..33236
                     /gene="folX"
                     /locus_tag="ACHKFK_000681"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO9051096.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33257..34150
                     /locus_tag="ACHKFK_000682"
     CDS             33257..34150
                     /locus_tag="ACHKFK_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO9051097.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLDLGHQITVVTRNPQKASSVLGPR
                     VTLWQGLAEQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKKL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34198..35088)
                     /gene="rpnB"
                     /locus_tag="ACHKFK_000683"
     CDS             complement(34198..35088)
                     /gene="rpnB"
                     /locus_tag="ACHKFK_000683"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO9051098.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGAGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDARRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMMEKGFD
                     HEVILTLTRLSPDDLIAQSH"
     gene            complement(35284..36057)
                     /gene="hisP"
                     /locus_tag="ACHKFK_000684"
     CDS             complement(35284..36057)
                     /gene="hisP"
                     /locus_tag="ACHKFK_000684"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO9051099.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36065..36781)
                     /locus_tag="ACHKFK_000685"
     CDS             complement(36065..36781)
                     /locus_tag="ACHKFK_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9051100.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36778..37464)
                     /gene="hisQ"
                     /locus_tag="ACHKFK_000686"
     CDS             complement(36778..37464)
                     /gene="hisQ"
                     /locus_tag="ACHKFK_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO9051101.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTREQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37554..38336)
                     /gene="hisJ"
                     /locus_tag="ACHKFK_000687"
     CDS             complement(37554..38336)
                     /gene="hisJ"
                     /locus_tag="ACHKFK_000687"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO9051102.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRIGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38557..39339)
                     /gene="argT"
                     /locus_tag="ACHKFK_000688"
     CDS             complement(38557..39339)
                     /gene="argT"
                     /locus_tag="ACHKFK_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO9051103.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGPSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39605..40174)
                     /gene="ubiX"
                     /locus_tag="ACHKFK_000689"
     CDS             complement(39605..40174)
                     /gene="ubiX"
                     /locus_tag="ACHKFK_000689"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO9051104.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAVTLPEDLFARWQGA"
     gene            complement(40269..41786)
                     /gene="purF"
                     /locus_tag="ACHKFK_000690"
     CDS             complement(40269..41786)
                     /gene="purF"
                     /locus_tag="ACHKFK_000690"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO9051105.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIEAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41823..42311)
                     /gene="cvpA"
                     /locus_tag="ACHKFK_000691"
     CDS             complement(41823..42311)
                     /gene="cvpA"
                     /locus_tag="ACHKFK_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO9051106.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42843..43505)
                     /gene="dedD"
                     /locus_tag="ACHKFK_000692"
     CDS             complement(42843..43505)
                     /gene="dedD"
                     /locus_tag="ACHKFK_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO9051107.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPLKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKEKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43495..44763)
                     /gene="folC"
                     /locus_tag="ACHKFK_000693"
     CDS             complement(43495..44763)
                     /gene="folC"
                     /locus_tag="ACHKFK_000693"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO9051108.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWTFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44833..45747)
                     /gene="accD"
                     /locus_tag="ACHKFK_000694"
     CDS             complement(44833..45747)
                     /gene="accD"
                     /locus_tag="ACHKFK_000694"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO9051109.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIIRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45903..46562)
                     /locus_tag="ACHKFK_000695"
     CDS             complement(45903..46562)
                     /locus_tag="ACHKFK_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9051110.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(46645..47457)
                     /gene="truA"
                     /locus_tag="ACHKFK_000696"
     CDS             complement(46645..47457)
                     /gene="truA"
                     /locus_tag="ACHKFK_000696"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO9051111.1"
                     /translation="MSDQQQPPVYKIALGIEYDGSRYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47457..48470)
                     /locus_tag="ACHKFK_000697"
     CDS             complement(47457..48470)
                     /locus_tag="ACHKFK_000697"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9051112.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATATWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGVMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGCPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48536..49672)
                     /gene="pdxB"
                     /locus_tag="ACHKFK_000698"
     CDS             complement(48536..49672)
                     /gene="pdxB"
                     /locus_tag="ACHKFK_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO9051113.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHERTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLSEGQKLSVVLDVWEGEPELNVELLKKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49771..50766
                     /gene="flk"
                     /locus_tag="ACHKFK_000699"
     CDS             49771..50766
                     /gene="flk"
                     /locus_tag="ACHKFK_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO9051114.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIEPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILILVWLVR"
     gene            complement(50763..51941)
                     /locus_tag="ACHKFK_000700"
     CDS             complement(50763..51941)
                     /locus_tag="ACHKFK_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051115.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVPAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALALTTMALPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSH
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52206..53426)
                     /gene="fabB"
                     /locus_tag="ACHKFK_000701"
     CDS             complement(52206..53426)
                     /gene="fabB"
                     /locus_tag="ACHKFK_000701"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO9051116.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53585..55591
                     /gene="mnmC"
                     /locus_tag="ACHKFK_000702"
     CDS             53585..55591
                     /gene="mnmC"
                     /locus_tag="ACHKFK_000702"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO9051117.1"
                     /translation="MKHYAIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEARFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLARADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGHVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFKAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFSMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSNKQEAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIHYQYQLQDLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSSLPVYSVAGQVSHIPTTPELAKLKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEETAYSEEDQLQNRQRLIDCFPHAQWAKEVDVSGKEARCGV
                     RCATRDHLPMVGNVPDYDATLVEYASLAEKKDEAVSAPVYDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSEEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55712..55990)
                     /locus_tag="ACHKFK_000703"
     CDS             complement(55712..55990)
                     /locus_tag="ACHKFK_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO9051118.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWEHLFQQASQQ"
     gene            complement(56024..56572)
                     /gene="epmC"
                     /locus_tag="ACHKFK_000704"
     CDS             complement(56024..56572)
                     /gene="epmC"
                     /locus_tag="ACHKFK_000704"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO9051119.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56572..57381)
                     /locus_tag="ACHKFK_000705"
     CDS             complement(56572..57381)
                     /locus_tag="ACHKFK_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO9051120.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALVGGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMS"
     gene            complement(57381..58205)
                     /gene="mepA"
                     /locus_tag="ACHKFK_000706"
     CDS             complement(57381..58205)
                     /gene="mepA"
                     /locus_tag="ACHKFK_000706"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO9051121.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSRQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPALWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58209..59294)
                     /gene="aroC"
                     /locus_tag="ACHKFK_000707"
     CDS             complement(58209..59294)
                     /gene="aroC"
                     /locus_tag="ACHKFK_000707"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO9051122.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59329..60261)
                     /gene="prmB"
                     /locus_tag="ACHKFK_000708"
     CDS             complement(59329..60261)
                     /gene="prmB"
                     /locus_tag="ACHKFK_000708"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO9051123.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALTVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            60427..60978
                     /gene="smrB"
                     /locus_tag="ACHKFK_000709"
     CDS             60427..60978
                     /gene="smrB"
                     /locus_tag="ACHKFK_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO9051124.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVDEWLPPKLP"
     gene            complement(61298..62155)
                     /locus_tag="ACHKFK_000710"
     CDS             complement(61298..62155)
                     /locus_tag="ACHKFK_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO9051125.1"
                     /translation="MKKTNLLFRLLILMVFMLGSRAGMGQTFRNVYIYYATFGWNSQV
                     ELYLDVAEVSSVYYGTYYQSNYVMTLGNLPNRTWTGTGSSPAPTITVKDGTIVNNSFC
                     PGMEALEAKDSRWECLKLPLDITVDGDVGGCPWLVSIRATSQVVVGSVDKDIYYGPNA
                     TVSVCPTVSVTPYDVSWDENYINKTKTLSLQSTGGVIEKTLSTYLMKDGKLCDGSQMN
                     EEGAYCRWVAQMITFSSSGCDNANVTVAPNRHPITDKQLHDMVVRVDTSSRQPIDSTC
                     RFQYTLNEL"
     gene            complement(62157..62684)
                     /locus_tag="ACHKFK_000711"
     CDS             complement(62157..62684)
                     /locus_tag="ACHKFK_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9051126.1"
                     /translation="MNLKRISLCAAVLATVLLTPHVQAAGNLHFYGNLLSKSCTLVVQ
                     GETLAEVHFPTISRKDLMVTGQSARVPVVFQLKDCKGPAQYEVRVTLTGTEDSEQPGF
                     LALDVSSAAQGVGIGMEKNDGTPVPINNTSGATFVLSNGNNNLNFNAWLQAKSGREVT
                     MGEFTASLTATFEYI"
     gene            complement(62681..63160)
                     /locus_tag="ACHKFK_000712"
     CDS             complement(62681..63160)
                     /locus_tag="ACHKFK_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9051127.1"
                     /translation="MKRLAWCLIYGFAGLAQAAINDVTFHGTLVSPPACTISDGKTIE
                     VEFRNVIIDNINGDNFRQDVPYTITCDPDVRDDAWEMSLTWTGSQTPYDDSAIETDVS
                     GLGIELQQNGQPFKLGTPLKIDPSTPPTLQAVPVKANDAALSDGTFSAYATLQVDYQ"
     gene            complement(63157..63660)
                     /locus_tag="ACHKFK_000713"
     CDS             complement(63157..63660)
                     /locus_tag="ACHKFK_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9051128.1"
                     /translation="MKAFFLTGSAALLLACGVTVFPAMAGGKIVNLTLRVLVDGPPPC
                     SVKGSAVEFGNVIIKSIDGANYRQAVGYTLNCSNSVSDDLQMQLQATTTTINGETVLS
                     TGISGFGIRIQNAADHSLVAVGNSSWLPFNINTQPDFEAVPVKQSGVELMASEFNATM
                     TMVVDYQ"
     gene            complement(63677..64429)
                     /locus_tag="ACHKFK_000714"
     CDS             complement(63677..64429)
                     /locus_tag="ACHKFK_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO9051129.1"
                     /translation="MSYKLSSPMLVSTALMAMLTTASLTAHASVTPDRTRLVFNESDK
                     SISVTLRNNNEKLPYLAQSWLEDEKGNKIISPLAVLPPVQRIDAMMNGQVKIQALPDI
                     HTLPSDRESLFYYNVREIPPKSGKANTLQIALQTRIKLFWRPKALEKIDMRKPWQFKV
                     TLTRSGQDYTVNNPTPYHVIISDASTQKKGLTAAGFKPLVMPPKTSQPLKAKMASAPV
                     LTYINDYGARMPLIFRCEGNTCKVDEDQSSKG"
     gene            complement(64449..67097)
                     /locus_tag="ACHKFK_000715"
     CDS             complement(64449..67097)
                     /locus_tag="ACHKFK_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001305198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO9051130.1"
                     /translation="MPNHSNFRLRGIACYIALAISGGSVNAWADDSIQFAPRFLELKG
                     DTKIDLGKFSKKGYVDAGKYNLRVFINKQPLSDEYDINWYVSENDPTKNYACLTPELV
                     AALGLKEGIAKSLQWTHNDECLKPGQLDGMEVENDLSQSALLLTVPQAYLEYTSSDWD
                     PPSRWDDGIPGLIADYSLNAQTRHQEQGGEDSHDISGNGTVGANLGAWRFRADWQSDY
                     QHTRSNDDDDDSSNSTTSKHWDWSRYYAWRALPSLKAKLSLGKDYLNSDIFDGFNYIG
                     SSVSTDDQMLPPNLRGYAPDVSGVAHSSAKVTISQMGRVLYETQVPAGPFRIQDIGDS
                     VSGTLHVRVEEQNGQVQEYDVTTASMPFLTRQGQVRYKVMMGRPEDWNHKTEGGFFSG
                     GEASWGVADGWSLYGGALADKHYQSAAMGVGRDLAQFGALAFDVTHSHVNLDHDSAYG
                     KGKLDGNSFRVSYAKDFDELNSRVTFAGYRFSEKNFMTMSEYLDANQSDMARTGNDKE
                     MYTITYNQNFAAAGVSIYLNYSHRTYWDRPEQTNYNLMFSHYFNMGSIRNMSISVTGY
                     RYEYDDNADKGMYLSMSIPWSDSSTVTYNGSYGSGSDSSQVGYFKRVDDATHYQVNVG
                     TSEQHGSVDGYLSHDGSLAKVDLSANYHEGEYRSAGIALQGGATLTAHGGALHRTQNM
                     GGTRLLIDADGIANVPVESNGAPVYTNMFGKAVVADINNYYRNQAYIDLNNLPEDAEA
                     TQSVVQATLTEGAIGYRKFKVISGQKAMAVLRLRDGSYPPFGAEVKNDEQQQVGIVDD
                     EGNVYLAGVNAGEHMMVFWEGSAQCEIVLPKPLPADLFSGLLLPCEQKGTAAPDSSAP
                     EIKPVIQDQTRQVTPTEAPTSISATQ"
     gene            complement(67178..67744)
                     /locus_tag="ACHKFK_000716"
     CDS             complement(67178..67744)
                     /locus_tag="ACHKFK_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9051131.1"
                     /translation="MSKFAKTAIAAAMVMGAVVSSSAFAAGNNGTARFYGTIEDSPCS
                     IVPDDHKLEVDMGDIGAAKLTGGGTTTPKDFQIRLQDCVFTTETNMETTFTGTNYAGP
                     ANTDNYALFNVDSGVAMNHVSLVIGDTHGKGYKVGEKIVQPIVMDTSTSKGKDKQTLN
                     FKAWLVGETDAPDLGQFETLTTFQITYL"
BASE COUNT        16597 a        18506 c        17089 g        15619 t
ORIGIN      
        1 cctctgatta ttttttacca accctgggtg aatgcattac cgccaacgcc ccgacacgca
       61 agtcctgagc aattagaaaa aacagttcgt tatcttacac aaactgtgca tccacgtagt
      121 gccgacaata ttgataatct gaataagtcc gcagagtata taaaagaggc ctttatcagt
      181 agcggagcca gagttaccgc gcaggatgtc cccattacgg gtggccccta caaaaacatt
      241 gttgctaatt atggtcctgc cgatggaccg ctgattatta ttggtgcgca ctatgactct
      301 gtcagtagtt atgaaaacga tcaattgacc tatacgccgg gcgcggatga taacgccagc
      361 ggcgtggcag gattactcga actggcacgt ttgttacagc agcagactcc agggattggc
      421 gtccaactgg tcgcctatgc gtcggaagaa ccgcccttct ttcgtagcga tgaaatgggg
      481 agcgcgatac atgcagcttc gcttgagcgt ccagtgaaat taatgatagc gctggagatg
      541 attggttatt acgactcaac gcctggtaac caggattacc catccccggc aatgtcctgg
      601 ctttatcccg atcggggaga ctttattgcc gtggtcggca gaatgcagga tatcaacgcc
      661 gttcgtcagg taaaagcggc attgttgtca tcccgggatt tatctgttta ttctatgaat
      721 gccccaggtt ttattcccgg tattgatttc tctgatcacc ttaattattg gcaacacgat
      781 attcccgccg taatgattac tgacaccgct ttttatcgta ataaacaata ccacttgccc
      841 ggtgatatcg cagacaggtt aaattatcag aaaatggctc aggtagtgga tggagttaca
      901 accttgttat acaacagtaa ataataaaaa tttctcgagg cggatccaga aaaagcaaca
      961 tgcgcatttt attttctttc gtctgtttta agatgcgaaa catgaaattc aacggaggaa
     1021 aaataaatgg gaatgattgg ctattttgca gaaattgact cagaaaagat taaccaactg
     1081 cttgagtcta ctaaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc
     1141 cgactggata ttgataaacg ctgggatttt ttacactttg ggctgacagg cacctctgct
     1201 tttgatcccg cgaagaatga cccgctgagt cgcgccgtgc ttggtgaaca cagcctagaa
     1261 gatggcatag atggcttcct gggattgacc tggaatcagg agttagccgc gacaatcgac
     1321 cgactggaat cactggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat
     1381 gaaatggaga tttatccggg agtaaccttt tcagaagagt tagaggggca actctttgct
     1441 tcgatcatgc ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg
     1501 aatcatgcgc tgacggttat tgttgggtga tggtgtcagg gcaccaaaaa aaaccgccga
     1561 acatgtcggc ggctttctga cttacaaact gactgtttac atcagcggca ttgctaaccg
     1621 cacaatgcta atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagagat
     1681 cagcaccacg ataccgcctg cgctgtactg ccagtttgat ggtgcatcgc gacccggttg
     1741 ttccggagcg tgcaggtata agctcaccgc cacgcgcagg tagtagtaaa ggccgattgc
     1801 cgaaccaaca accacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag
     1861 cacgtagaac ttaccgataa agcccagcgt cattgggata ccagccagtg aaagcatcat
     1921 caccgtcatc actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag
     1981 ggaatcagca tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc
     2041 gaggctgctg aacagataac cgaccaggta aacccctacc gcttccatcg acatctcgcc
     2101 ggtttgcagc gcaatcagcg ccaccagcag atagccgagg tgagagatag atgagtaacc
     2161 gagcagacgt ttgatattgg tctggctcag cgccatcagg ttaccgaaga tgatggaggc
     2221 aaaggcgata atcgccagca ctacgcgaat cgcttcactg tcacccaccg gtgcgtaaag
     2281 gaacaaacgc atcaccacgc cgaagatagc gattttgctc gccgtcgcca ggaaagtgga
     2341 aaccggcgca ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagaga
     2401 gagtttgaaa ccgaggccaa caatcatcag gccaaaacct gccagcaaca gcggctcgtt
     2461 gagcataccg tcgccgaggt ttttacccag tgcgacaaac gacaggtcgc cagactgcgc
     2521 atacaccagc gccataccga acagcaggaa agaagacgct gcggcagaaa ggatggtgta
     2581 tttgatactg gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag
     2641 cggcaaagag atcagttcga taccgaggaa cagagacgcc agatggttgg cattcgccag
     2701 caggatcccg cccagcgcgg caattaacac caacaggtag aactcgtcct tgttgtcgtt
     2761 atagccttca agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag
     2821 cccggtgtaa agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc
     2881 cgcctggccg acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt
     2941 agcgttgagg aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa
     3001 gccgacgatc agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat
     3061 ttacggcctt gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga
     3121 gtgcgaggta tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag
     3181 caacaagatc ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg
     3241 gcttttcgct ttgccgaagt aagcgcgatg cagcatcgcc agcgaataaa cagatgcaaa
     3301 gaccagccca aaggtagaga taacggtaat caccgggaca acctggaagc tgccgaacag
     3361 aatcataaat tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa
     3421 gaacagcgac agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat
     3481 gtcgcgggta tggatacgtt cataaagctg accacaaaga ataaacagac ccgccgccga
     3541 caagccgtgc gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta
     3601 gatagcaatc agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat
     3661 atcggtctgg gcgaaggcca tccacgcacc atagaagatg ccgataacac ccagccacat
     3721 agcgattggc gcgaactctg ccgacgcgtt cgggaacagc ggtagggaga aacgcagcag
     3781 accgtaagcg gcggttttca gcaagatccc cgcgagatca acggaaccgg cggtcggtgc
     3841 ctgggagtgc gcatccggta accagccatg cagcggaacc accggcattt tcaccgcgaa
     3901 ggcgatgaag aagcccagca tcaacagata ttccacgccg ttagacatcg gcgtattcag
     3961 cagctcttca tagttgaagg tccagacgcc ggtcgcattg tagtgaacga aaaccagcgc
     4021 caggatggcg atcaacatca ccagaccact cgcctgggtg taaatgaaga acttggttgc
     4081 cgccgtgata cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta
     4141 catcggcacc agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa
     4201 cacgccgata acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt
     4261 ttcgatctct ttccacgaac agagtaccgc cagcacaccg agcagaccgg tcagcacgac
     4321 catcagcaac gacagaccgt caatggcgag atggatagag ataccaaaac gcgggatcca
     4381 cggcatgtcg aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc
     4441 gccctgcaac cacagttgca gcgacagcgc cagcgtcaat cccatggtta ccagcgcgat
     4501 ccagcgcggc accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccgataaa
     4561 gggaattaat attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt
     4621 caggaccgga ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg
     4681 tcggatgcgc cgcgaagacg gcttatccga cctacgttct tatatgccca ggggcaatcc
     4741 cacaatcctt aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc
     4801 tcatggatgc cacataccag cgcagataac cgttctcgct taacagcaga cctttacctg
     4861 caaagcggga aaggacagcc gggatgttca tcattgagtt cagcggatcg cgtttcagca
     4921 accaggcaat acccaggaac ggcttgacga acactttgtc gtacagccag tcaaagcccc
     4981 aggcgttgta ccaccaggtg cccagcagac ggcccggcga actgttggcg atggaggtca
     5041 ccagagtacg tttacccagc cacagccagg ctgccagcag aatgccgacc accgcgacca
     5101 cgccagaggt aatttccagg gtcaacatgc tgccgtgcgc cagctccgtc gtttgcggaa
     5161 gcacgccctg cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg atcagcagca
     5221 caatcagcgg caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc
     5281 cgtggaagac gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca
     5341 gaccagccac catcagattg atatgaccat tcgccatcgc acccgcgagg atctcatcct
     5401 tactgaagaa gcccgcagtg accagcggca gtgccgacag tgctgcgccg cccaccagga
     5461 agcagagata aaccagcgga atagatttac gcagaccgcc catcttgaag atgttctgtt
     5521 cgtgatggca ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg
     5581 cgtgagtcat caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca
     5641 tgtagccaat ctggctcatg gtagagtaag cgagaacacg tttgatgtcg gtctgtacca
     5701 gcgcggcaaa accggccagc agcagcgtaa ccgccccgac aatgcccacc agatgcagaa
     5761 cttccggcgt catcaggaac aggccgtggg tacgggcgat caggtagaca cccgcggtta
     5821 ccatcgttgc ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcgacaa
     5881 gccatgtctg caacggcaac tgcgcagatt taccgaccgc accgcccagc agcatcagcg
     5941 tcgcccacat cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt
     6001 cgcggaagtt cagggtgccc agttcgttgt aaagaatgaa cagcgcgaaa gcgaggaaga
     6061 cgtcacccac acgggtcacg acgaacgctt tcattgccgc tgcgccattc ttcggatcgg
     6121 tgtaatagaa accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca
     6181 tcagcagcag gttgtcggca agcaccagaa ccaccatgct ggcgatgaac aggttggtgt
     6241 aagcgaagaa gcgagagtag ccctcttcac cgcgcatata ccaggaagcg tacatgtgaa
     6301 taaggaaacc cacaccagtg accactgaga gcatggtcag cgacaggccg tccagcacca
     6361 gattgaaacc gatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg
     6421 cctgctcacc gttagcgaag aagtcaacgc cgataaaggc ggttaccagc gccgccaggc
     6481 ccacagagcc aacgccgacg atcgccgaga cgttttcaga ccaacgccca cgggagaacg
     6541 ccagcaggac gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg
     6601 catctcactt actgaatcaa tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag
     6661 cgcaaggccg atactcgctt ctgccgccgc gaggctgatg gcgagaatgt acatcacctg
     6721 accgtcggtc tggccccagt agcttccggc gaccacaaag gccagcgcgg aggcgttaat
     6781 catgatttcc agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa
     6841 gccaagaacg aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc
     6901 tcctccgttt ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc
     6961 tcttcacgac cgacgtggaa ggccacaacc agacccgcga gcagcagcat agaagccagt
     7021 tccaccgcca gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc
     7081 gtaccgtcga taccttgatc gttaacaccg agaatggcgt aaacaatcac caccagcatg
     7141 atggctgaca aaattgccgg accaatccac acctgcggtt tcagccactg gcgttcctgt
     7201 tcgatttctg aaccgcccag gttgagcatc atcaccacga atacgaacag caccataatg
     7261 gcacccgcat agacgataat ttccagcgca ccggcgaagt aagcgcccag tgagaagaac
     7321 accccagaga tcgccagcag cgaaataatc aggtacagca gcgcgtgtac cggattggta
     7381 tgggtgatca ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac
     7441 tccattgccc ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct
     7501 ctgcttcgcc cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt
     7561 ccgggtattt gcccggaccg gagatcagca gatcctcttt ctcgtaaacc agatcctggc
     7621 gcttgtattc ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt
     7681 cttcgcacag accacagaaa atgcagcgtg agaagttgat gcggaaaaat tccggatacc
     7741 agcgaccgtc tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg
     7801 cgcagaggtt acaggctacg caacgctctt cgccgtccgg atcgcgggtc agaacgatac
     7861 gaccacgata acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt
     7921 tggcgaacgc gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta
     7981 acaattcttt taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc
     8041 cgccgttacc agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat
     8101 tacctggtca taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa
     8161 gaacgcggtt ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc
     8221 gaagaacagc gtcaccatca gtgcagagat ggtcacaatc ccgatgtatt cgcccacgaa
     8281 gaacagaccg aacttcatac cggaatattc aatgtggtaa ccatccgcca gttcctgctc
     8341 ggcttccggc tggtcaaacg ggtgacggtg acacaccgcc acgcccgcga tggcaaaggt
     8401 aataaaacca aagaattgcg ggatgacgtt ccacacatgc gcctggctgt tgacgatgtc
     8461 ggtcatgttg aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac
     8521 ttcgtagctc agggtctgcg cagaagcacg catcgcaccc agcagcgagt atttgttgtt
     8581 acttgaccag cccgcaaaca gcaccgcgta aaccgccaga cctgccatca tcaggaagaa
     8641 caaaatcccg atgttcaggt cggcaaccac ccaacccgga ctgactggca caattgcaaa
     8701 ggccagcagc agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cgcgatccga
     8761 gaatttcggg atccagtctt ctttaaagaa cattttgatc atgtccgcaa ccagctggag
     8821 cgaaccgccc cagccaacac ggttaggtcc aaaacggttc tggaacagac ccagcaggcg
     8881 acgttcgcca aagctcatga atgccccaca ggtgactacc accagcagga tcaccaccgc
     8941 tttaaggacg gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc
     9001 tccttgagat cctcaagatg cgcgccagcc agcaccggag caatgccgga catacccatc
     9061 ggcaagccca cctgccctgc tgtcagccct tcggcgattt caaccggcag cgtgaccgtg
     9121 ttgccatcgt aactaaagga gacgcgtgta cctgcgttca cacccaactt cgcggcatcc
     9181 gctgggttga gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt
     9241 gacaattcat cgctgccaaa caggtggtag tacggcgcga tacgccattt cccgtcctgc
     9301 ggctggaagc gtgccggtac gctggtgaag taatccagac cgttttcgct ggtttcaaac
     9361 agacgcacgc ccggatcgcc aaaacgcagt ttaccgccca cttcgtcctg gaatttgttc
     9421 cacgcctgcg gggagttcca gcctggtgcc caggcaaacg gcacttgcga acggtgcgcg
     9481 gtcggctggt tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggttga
     9541 cgcggctcat gaacgctgat attggcgcgc atggcggtac gaccgctata gcgatgcggt
     9601 tcacgggcca gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata
     9661 cccgccagtt ccgggatttt cgccacaaca gcgtcaatca catggtcgag ctgtgtccag
     9721 tccacttcac ggctcagcag ggtgctgtgc agcgagtgca gccagcgcca gctttccagc
     9781 atgacagttt ggctgtcgta ataagccgga tcgtaaacct ggaagaaacg ttgggcgcgg
     9841 ccttcgttgt tgatcaccgt accgtcgctt tcagcaaagc tggcggcgga aagtaccaga
     9901 tgagcgtttt ccataatcgc tgtgcgttga tggtcaacca ccatcaccag cggcgcttta
     9961 gccagcgcag cattcacgcg ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc
    10021 accaccgcgt cggcgcgtcc ggtttccagt tcagttaacg cttcttcaag cgatccaccg
    10081 cctataatgc ccagccccat gctgttgacg gaacgggcaa tcatggtgat accgacgtca
    10141 gcgccgcgac ctttcagggc tttcgccacg ttagccgcag cctgaatcac ttcagcgcta
    10201 ccagcgttcg tcccggagat aatcaacggt ttcttcgcac cagccagtgc ctgcacgatg
    10261 acgtcgattt tgctttgcag ctcaggttcg ataccgtcaa ccgctggcgc ggagttatcc
    10321 agcgcatggg cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc
    10381 cacgcagcga tatcatccag acgggtgtca tcaacgttgg taacaaacag tggatgcttc
    10441 gcacgttgac cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgcc
    10501 gccatttcgc gtgctttacc ttttaccgcc tggcgcaccg ccagcgcaac gcgcgcgccg
    10561 gtctgggtaa cgtcttcacc cagcaccagt accgcatcgt aagattcgat ttcacgcaga
    10621 gccggagtat aaatgccgcc ttcacgcagt actttcagcg ccagttgcag acgttcctgc
    10681 tcaccgtgag cgataccggt atagaagttt tcttcgccca ccagctcacg cagcgcaaag
    10741 ttgctttcca cgctggcacg cggagaacca ataccgatca ctttcttcga ctgacgcaga
    10801 atatctgctg caccctgcat tgcctgttcg gcgttgagga taatgaaatc atcgccacga
    10861 cgctgtactg gctgacgcgg acgatctttc aggttgacgt aaccgtaacc gaaacgacca
    10921 cggtcgcaga ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat tcgacgcagt
    10981 tcgccataac gctcgccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc
    11041 ggcgcaaact gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtgaat
    11101 acgccggtcg ggcaaatttc gaccaggtta ccggaaaatt cgctttccag cgtgccgtct
    11161 tccgggcgac cgaagtagac gttgtcgtgc gcaccgtaaa cgcccaggtc cgtgccgtca
    11221 gcgtaatctt tgtagtaacg cacgcagcgg tagcaggcga tgcagcggtt catttcgtga
    11281 gagatgaacg gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg
    11341 aagctgtgtc cggtcatcac ggtcatatcc tgaagatggc agttaccgcc ttcttcacat
    11401 actggacagt cgtgcgggtg gttggtcatt aaccactcga ccacactttc acggaactgt
    11461 ttcgcttctt cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac
    11521 atcaccaggc gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg
    11581 caagcaccga cgcttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga
    11641 gacagacaag cttccagcag gttgtccgct ccgttgacct cgtattcttt gccgtctaca
    11701 tgaattgtag ccattagcat gcttccagtt ttctcagtca gagactgagc gttaatcgaa
    11761 attcggttac cagcgctctt tcagcaggtt cggctgaatc ccattaatca aatgggtatt
    11821 gctgaacggc tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg
    11881 taacggctcc actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg
    11941 acacagttgc tcaagtgttt cgatatcgcc cggctggcct tcgccacgct ccagcgcacg
    12001 cagaattttc acgctccacg gcagaccgtc gcggcatggc gtacaccagc cgcaggactc
    12061 acgggcgaaa aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac
    12121 cgccatcgcc agcgccgtac ccagacggct tcccgcttta ccgatacttt cgaattccat
    12181 cggcaggtca aggtgcgctt cggtcaggaa gtcagtcccc gctccgcctg gctgccaggc
    12241 tttgaatttc agaccgtcac gcataccacc ggcgtagtct tcgaggatct cgcgtgcggt
    12301 ggtgccgaac ggcagttccc acagacccgg atttttcacc cgaccagaga agcccatcag
    12361 cttggtgcca gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag
    12421 gatcgccgga acgttacaca gggtttcgac gttgttaacg caggttggtt taccccatgc
    12481 accggaggtt gccgggaagg gcggtttcga gcgtgggtta gcgcgacgcc cttccaggga
    12541 gttgattaac gctgtttctt caccgcagat gtagcgccct gccccggtat ggacgaacag
    12601 ttcgaaatcg aaacctgttc ccataatgtt tttgccaagc agacccgctt cggtggcttc
    12661 ggcaatggca cgacgcagat tcactgccgc ttcgatatat tcgccgcgca ggaagatata
    12721 gccacggtaa gctttcagcg caaacgcgga gatgagcatg ccttccacca gcaggtgcgg
    12781 cagttgctcc atcagcagac ggtctttata ggtgcccggc tccatttcat cggcgttaca
    12841 cagtaggtaa cggatattca tggattcgtc tttcggcatc aggctccact tcaggccagt
    12901 cgagaagcct gcgccaccgc gccctttcag accagagtct tttacctgat taacgatttc
    12961 gtccggagac aacccggtca gcgccttacg cgcgccttcg taaccgtttt tgctgcgata
    13021 ttcgtccagc cacactggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc
    13081 gggagtacgg ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg
    13141 gtcagatgcg cgtgagtgtc ctcatcgatc atcatgtttg gccctttatc acagttcccc
    13201 aggcagcaag ttggcagcaa cgtgaagcga ccgtcaaatg tcgtttgccc tggtttgatg
    13261 ttcagttttt tctccagcgc cgcctgaata ccctggtaac cgttgatgtg acagaccacg
    13321 ctgtcgcaat aacggatcac atggcggcct actggctgac ggaagatctg gctataaaac
    13381 gttgccacgc cttcgacgtc gctcgccgga atacccagca catcggcgat cgcgtggatc
    13441 gcgccatccg gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc
    13501 gcacgcgggt cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc
    13561 agctcaaaag cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg
    13621 acataacaaa atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga
    13681 tcgccgccgg aatttgctgc aaatgcgcat aactcggcgt acggatacgg gtgcgataac
    13741 tcatggtgct gccgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaacca
    13801 tctggaagga ttcattggca ggcatcaccg gaccccacga cacttgcagg aagtgggtga
    13861 tcagggtttc gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat
    13921 ccgctttgaa cgggccttcc ggcatgttgt tgaggcactg ctcaagaata cgcagactct
    13981 ggcgcaactc ttctactttc agcattacgc gggtgtagca gtcagaaaca ccaccgccca
    14041 ccggaatttc gaagtcgaag ttttcatagc cagaataagg acgcgcctta cgcacgtcga
    14101 agtcgatccc ggtagcacgc aggcccgcgc cggtggtgcc ccactccagc gcctctttcg
    14161 cgccgtaagc ggcaacgccc tgggaacgac ctttcagaat ggtgttttgc agcgccgctt
    14221 tctcgtaaga cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc
    14281 agccgcgcgg caggtcgtgc gctacgccgc caatacggaa ccacgccggg tgcatacgaa
    14341 aaccagtgat tgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca
    14401 ctggcgtcat cgcgccgacg tcctgaataa aggtggagat atacagcagg tgactgttga
    14461 tgcggaacag ttcggagagc attacgcgaa tgacgttaac gcgatccggc acggtgatcc
    14521 cagccagttt ctctaccgcc agcacataag gcatttcgtt aacgcagccg ccgaggtatt
    14581 cgatacggtc ggtatacgga atgtagctgt gccaggactg gcgttcgccc attttctctg
    14641 cgccacggtg gtggtaaccg atgtctggta cgcagtcgac aatctcttcg ccatcaagct
    14701 gcaaaacgat acggaaagca ccgtgcgcag acgggtggtt cggaccgagg ttgaggaaca
    14761 tgaagtcctc gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg
    14821 cctccatctc cagatcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc
    14881 gcgccgggta atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc
    14941 gcaggttcgg gtgaccgtcg aatgtaatgc caaatagatc ccaggtttca cgctcatacc
    15001 agttagcgtt cgggaataat ttggtaaagg tcggtacgtg caggtcgttt tctgccagcg
    15061 ccaccttcag catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa
    15121 aatccgcggc aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt
    15181 caaacagcat gacgtaaggt ttcggcagtt tctttaagaa atcgcccact tccagtaatt
    15241 gttcacgctt gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat
    15301 ccggcccaaa acggttgcgc agttcgccaa tcaccggatc atcaagatga tcgcgggtct
    15361 gccaggcggg ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt
    15421 ggtgactgtc ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaattcc
    15481 ctttgccgac aggcgcaaat taaatctcgt caggtgtacg caggttggta acggcaatgc
    15541 gttcaccgcg cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc
    15601 caaccaccca ggagagcgga cgacgttctt taccgataga ttcctgcaac agcatcagtg
    15661 cctgcatgta cgcttcagga cgcggcggac agcccgggat atacacatca accgggatga
    15721 atttatcaac gccctgcaca acggaataaa tatcgtacat accgccggag ttggcacagg
    15781 cacccattga gataacccat tttggttcca gcatctggtc atacaaacgc tgaataaccg
    15841 gtgccatttt ggtaaagcag gttcctgcaa ccaccatcag gtcagcctga cgcggcgaag
    15901 cacgcaatac ttcagcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca
    15961 tctcaacgta acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc
    16021 agttaaccat gtcattgagc ttgcccataa acacgttttt gttaacttct tgctccagag
    16081 ggtcggttac gatctcctgc ttttgcaggg ggtaacggtc attctcaccg ttgggatcta
    16141 tgcgggtgag cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac
    16201 tgttcgtttc cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc
    16261 caatacgcac cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca
    16321 caaagcctac ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt
    16381 caacgtcgaa gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc
    16441 gggcggagcc gaccgagtcg ataccggatt caaacggaac gttttttgac ctcgcgcgtg
    16501 cgcgaccgcc taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga
    16561 taagaaagat agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca
    16621 ttgcttactc atcaaaagta gcgcctgatt ctctgctctt tacggcagat ggacgccaca
    16681 tcgattcatg gggaggaata aaaaaaacct tacaataact gcagaaacta ttttatacag
    16741 ctaattgatg tggtctttta ctcctttcta taaccttttg tcaactttaa caaaggtttc
    16801 ttcacactga tttacacaat gacaacaaca tttgcatttg gcaccctttc tccccagatc
    16861 attgacgact ccaggaaaat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa
    16921 aaatacgggt ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt
    16981 tcttgatctg tgacacgctt ttgtcattct ataacaaaaa tgcagcaaca aattcacgta
    17041 ttttttaaca ttattgtagc aggagatttt tttcaggtga ttatttgtgc gtacggggcg
    17101 tgaatctctg gtgattgaaa aatgaacagt tttgtacgtt ctgcacgata cgaggaaggc
    17161 tttttactaa aaaaaagccg ctggggttta aaataaaccc agcggttcgt ttttacacta
    17221 ttgtctcagg aattatctat cgtccgtcga ttactcgata tccctttcaa tcaacaatga
    17281 gtcatctcct tccggagcag acattgggct gtattgccac ggattatggt agctctccat
    17341 cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata
    17401 ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca ggtccggact
    17461 catcatctcg accggccttg ccgtcacgcc aagaccggct ttcactgccg cacgaacggc
    17521 cggaagcgtc gatgcgacat aagccagtcg ccagggaata tctgctttat taagcgtcgc
    17581 cagcaccata tcgcgaaacg gacttggatc atccagcaac acaagaggga tcggctcacc
    17641 tttttgcaat acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa
    17701 tgccttaaag gtagaaggac gatgggtggt taccatcaaa tccacttcct gggattccag
    17761 catttctgcc atgtaggcat tacgtttaac gcggacatcc agcgctagtt tcggataaac
    17821 cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc
    17881 gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa
    17941 acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt
    18001 gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg
    18061 acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc
    18121 ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatca gacgatttgc
    18181 acttatcata tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac
    18241 acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg
    18301 aaagtaaaaa tgcatatgag ttgcactaaa aaagtgactc acattgttcc gttataatgc
    18361 ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt
    18421 ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc
    18481 gtacagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg cggcatcaag
    18541 agcagggatc cgtcatttat gcaagcattt taccccaaaa accttttatt tataagggtc
    18601 attgcgaatt atctgatgca aagttatgtt atgttaggca aagtaatctt ctattattaa
    18661 taagcacaac aaaacctttt tgaatattaa ataataatta attagcatca tccccattca
    18721 ttaattccgc ttaacaatag tttcacgcct tgcccccatt gtgacacctc ggaaatcatc
    18781 acgctgttaa actctccgta atgcggaaat atcatcatcg cgattcagta gtgaattctt
    18841 aaacgaggca ttttcacaca attatcttac agataaaaaa accacactta caattaagaa
    18901 tcagaacaat tcactacata acattactta ttaagcatat gcacctcatt attttgccat
    18961 tattaagtta ttaacagcac aatcgagcct tcccctctgg caaaatctta ttctgcagac
    19021 cttcaaaaca ccgtcctggg ggagtacatt gttctaagct gacttccacg gcagggagtg
    19081 gcgataacag caaaaaaggt caagattcat gtcccccatt gaaaaatcca gcaaattaga
    19141 gaatgtctgt tatgacatcc gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga
    19201 aggtaacaag gtactgaaac tgaacatcgg caaccctgcc ccgttcggtt ttgacgcgcc
    19261 agatgaaatt ctcgttgacg tgatacgcaa cctgcctacc gctcaagggt attgcgattc
    19321 caaaggtctt tactccgcgc gtaaagccat catgcagcac taccaggctc gtggcatgcg
    19381 tgatgttacc gtggaagata tttacatcgg caatggtgta tcggagctta tcgttcaggc
    19441 aatgcaggca ttgctgaaca gcggggacga aatgttggtt cctgcacctg attacccact
    19501 atggaccgca gcggtttcgc tctccagcgg taaagcggtg cattatcttt gcgatgaatc
    19561 ctctgactgg ttcccggacc tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg
    19621 gatcgttatt atcaacccaa ataacccaac cggcgcggta tattccaaag agcttttaat
    19681 ggagattgtg gagattgcac gtcagcataa tctcattatc ttcgccgatg aaatttatga
    19741 caaaattctc tacgacgacg ctgagcatca ctccattgcg ccgctggcac ctgacctgct
    19801 gaccattacc tttaacggac tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg
    19861 gatggtgttg aacgggccga aaaaacacgc caaaggttac atcgaaggtc tggaaatgct
    19921 ggcttcaatg cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga ccgcactggg
    19981 tggttatcag agcatcagtg aatttattac ccctggcggt cgtctttatg agcagcgtaa
    20041 ccgcgcgtgg gaattgatca acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc
    20101 gctgtatatg ttcccgaaaa tcgacgccaa acgctttaac attcacgacg atcagaaaat
    20161 ggtgctggat ttcctgttgc aggaaaaagt cctgttggtg caagggacgg cattcaactg
    20221 gccgtggccg gatcacttcc gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc
    20281 tttgagcaag ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt aatttcactg
    20341 ccggagattg catccggcag tgttatcccg ccacaatgac ctgatgatgt catcatacgt
    20401 aaggtcacta tgaaacagag ccattttttt gcccatctct cccgcctgaa actcattaac
    20461 cgctggccgc taatgcgcaa cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta
    20521 gcgatggtcg cccatgcgct ggcagctatc aaaaatcgta aatttggcgg taatgtcaac
    20581 gccgaacgta tcgctttact ggcgatgtac cacgatgctt cagaagtgct caccggagat
    20641 ctccctactc cggtgaaata cttcaattcg caaatcgctc aggaatacaa ggctattgaa
    20701 aaaatcgctc agcaaaaact ggtcgatatg gttccggaag agctgcagga tatctttgcg
    20761 ccgttaattg acgagcatgc atatagcgat gaagaaaaat cgctggtgaa acaggcagat
    20821 gcactgtgtg catatctgaa atgcctagaa gaacttgcgg ccggaaataa tgaattcttg
    20881 ctggcaaaaa cgcgactgga agcgacgctt gaagcgcgcc gcagccaaga gatggactac
    20941 ttcatggaag tatttgttcc cagcttccat ctttcgctcg atgagattag ccaggattca
    21001 ccgctgtaag cagccggagt ctgcgtcgca tcaggcaata agcaccggat gcgacatcag
    21061 gctcttgtca aaacggaaac agcatcggga tcatcaccac acaaaccgcc atcacgataa
    21121 tggtgaacgg tacccccaac ttaacaaagt cactgaagct gtaattcccc ggacctaaaa
    21181 ccagtgtgtt gacaggtgaa gatatcggtg tcataaaggc ggcggatgct gccatcgcca
    21241 cgaccatcgc aaatggataa ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg
    21301 gagccatcaa caccgccgtc gcggtattag aaataaatag cccaataacc gccgacaaga
    21361 caaacaaaca acccagcatc atatgtggcc cgtaaccgcc gccaatgtcc atcagccctt
    21421 tcaccaccag cgcgacacct cccgttttct gtaatgccac agcaaatggc atcatcccaa
    21481 cgatcaaaat aatgctcggc cagtgaatgg atttataggc gctttcagca tctatacagc
    21541 ggaatttccc catcagcagg caggcgataa tagcggcaac aggattagga atttcatcgg
    21601 tcagcattaa cgccaccatt agcaccagac agaaaatcgc atggggtgcc tggctgtgcg
    21661 cgggcgatgc ttcactcacc tcttccggta agttcagcgc tacgaagtcg cggccctgtt
    21721 tggccagcat accgatcagt ttccagttac ccacaaccag gatgatatcg cccagcagca
    21781 gaggctcatc cgccagcgaa ccttccagcg ccacgccatt gcgctttagc cccactacat
    21841 tcaatccgta gcgggtacga aaaccaattt cgcgcaccga tttaccaata agttctgact
    21901 caggaattaa tgaaatctct gccatgccca catcaagggc ctggtcagaa aaatactcgc
    21961 cgcgcagtac catcggctcc agcaattgct cactacaaaa ttgccggaga tcgacatcag
    22021 ccgcagacat atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt
    22081 tcacgataac gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta gcaccataac
    22141 gttcacgtaa tttgagatcg tccagccgtt gaccaatcat tggcgatccg gggcgaatag
    22201 ccagacgtcg cgcacgcccg gtcagtcgat attcacggat aagatcgcga aaggttcggc
    22261 gcgtccagcc ttcgcgctgc ggggtctggg tatccccttt cagcatgaaa cgcatcacta
    22321 acatatacaa aatacccagc accagcacga ccaggccaat aggtgttacg ctaaagaaac
    22381 tgaagccgtg atagccttca cgcagcaatt cactgttgac taccaggttc ggcggcgtcg
    22441 ccaccagcgt catcatgccg cttatcagcc cggcaaaact taacggcatc atcagacgcg
    22501 acggcgacgt ttgcatacgc atggcaacgc ttaacaccac gggaataaag atagcaacaa
    22561 cgccggttga actcataaac gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca
    22621 ttttgatttc actgttgccc gccactttga ccagccatgt ccccattaca gtggcaacac
    22681 cggtacggac caaaccatcg ccaataatga acaaggcggc aatcaggaca acgttaggat
    22741 cggaaaagcc ggaaaatact tcggggaccg tcagcgttcc gcttaatgca aacgcgacaa
    22801 taacaaacaa agcgaccgca tccatacgca ctctgcccgt cgcaaacaag acgatggcaa
    22861 cggccagtaa tgaaagaacc caaatcaatt caccgttcac aacttatcct tgttaattga
    22921 gggggataac ttgattctgc cataaaaaag ccccgacgag acggggctaa atcatgatca
    22981 ggtgtttcac tgaataataa catcgccatt tggctgtttg gtcacagtaa tttgctccag
    23041 actgtggagg actaaatcga cttcattcag gcgcggggta tctgccgggg cattaaccgc
    23101 aataacatgg caacccgccg ccagacctga aagcacgcca gcgggagcat cttccaccac
    23161 aacacactcc tgcggcgcaa gccccagcag ctgcgcgcct aacagatacg catcaggttc
    23221 tggttttccg cgcttcaccc gctcagcggt tacaaacacc tctggcgcgg gaagccccgc
    23281 tattttatga cgcgctcgcg ctaccggcat ggagccagaa gtcacaatag cccacggaat
    23341 acctgcttta ttcaaatgat tgagtaaggc gattgccccc ggaagcgcgg taatgccttc
    23401 ggtttccgtg gcctcaatgt gctccagacg cgtaaactcg gcggcaatgt ctgcctcgga
    23461 tttgcccgcc ataaaatggc gcagagaggt gatcgcctgt ttaccgtgaa tgaaagccag
    23521 cacctcttcc ggcgataacc catgacgtct ggcccagttg ctccacgccc gttctaccgc
    23581 aggcagggaa tccaccagcg ttccatcaag atcaaacaga aaacctttgc accgcacgcg
    23641 ggcctcctca ggcgttaata atttgattaa tttcgttggc gctcaaatgg tactgacgcg
    23701 ggcaggcatg ccagacatta agcatgcgct gatatttttc ccacattggc gtctgggcgt
    23761 taaaaccgtg agttccagcg tcaaaatggg tatagcgccc ttccacatta accataaagc
    23821 gaacataacc gaggtaacgt gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac
    23881 gttcatcgat ggattgctgg tcctgcaaat tagaccagga aacatgcaac gcatgataca
    23941 tctccataat gtcgatgata gtgcggcagg tttcttcttt cagctcgcca aactcgcgat
    24001 ccagttcacg catctgtaat ccataaccac gctcaatgat tgtttgcagg cgacggtaac
    24061 gttcagcatt tgccggatcg agcatagtca tcatcttgta ctggttagac aaaataagac
    24121 gttgcgcgtt ggtcatttcc attattgact cctgtatcac tctactacgg tgaaaaaaaa
    24181 gaaggctgag tatgccttct tttatatgcg taatcagggg tcaattacaa atcatcaagg
    24241 aaagttttat ccagttgttt gaaggcacgc ttaagcgtgt cagctaatgc ctggtaatca
    24301 ggcttgcctt caacgggtgc caacacctgt ccagactcct gcaatttacc acgaacttca
    24361 taaaaccagt tgaggattgc aggaggtaat ggcgttacag aacgcttgcc cagccaccac
    24421 aatccctgca tgggtaaact taaggcgaac agcgcagtgg caactgccgg cccaagctga
    24481 ccgcccaggg caatctgcca gcagagagta aatacggcga tcggcggcat aaaacggatc
    24541 gcataacgcg tcatcttgat aacgcgattt tcgacaaaga ctggggcaag gcgtttttcc
    24601 agcggccacg tctttgagta atgctgtccc cggcgaaaca agctaaaaaa attaacagaa
    24661 cgattatccg gcgttgacat gcttcacctc aacttcacat ataaagattc aaaaatttgt
    24721 gcaaattcac aactcagcgg gacaacgttc aaaacatttt gtcttccata cccactatca
    24781 ggtatccttt aacagcctga aggcctaagt agtacatatt cattgagtcg tcaaattcat
    24841 atacattatg ccattggctg aaaattacgc aaaatggcat agactcaaga tatttcttcc
    24901 atcatgcaaa aaaaatttgc agtgcatgat gttaatcata aatgtcggtg tcatcatgcg
    24961 ctacgctcta tggctccctg acgttttttt agccacgtat caattatagg tacttccatg
    25021 tcgagtaagt tagtactggt tctgaactgc ggtagttctt cactgaaatt tgccatcatc
    25081 gatgcagtaa atggtgaaga gtacctttct ggtttagccg aatgtttcca cctgcccgaa
    25141 gcacgtatca aatggaaaat ggacggcaat aaacaggaag cggctttagg tgcaggcgcc
    25201 gctcacagcg aagcgctcaa ctttatcgtt aatactattc tggcacaaaa accagaactg
    25261 tctgcgcagc tgactgctat cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc
    25321 tccgtagtga tcgatgagtc tgttattcag ggtatcaaag atgcagcttc ttttgcaccg
    25381 ctgcacaacc cggctcacct gatcggtatc gaagaagctc tgaaatcttt cccacagctg
    25441 aaagacaaaa acgttgctgt atttgacacc gcgttccacc agactatgcc ggaagagtct
    25501 tacctctacg ccctgccgta caacctgtac aaagagcacg gcatccgtcg ttacggcgcg
    25561 cacggcacca gccacttcta tgtaacccag gaagcggcaa aaatgctgaa caaaccggta
    25621 gaagaactga acatcatcac ctgccacctg ggcaacggtg gttccgtttc tgctatccgc
    25681 aacggtaaat gcgttgacac ctctatgggc ctgaccccgc tggaaggtct ggtcatgggt
    25741 acccgttctg gtgatatcga tccggcgatc atcttccacc tgcacgacac cctgggcatg
    25801 agcgttgacg caatcaacaa actgctgacc aaagagtctg gcctgctggg tctgaccgaa
    25861 gtgaccagcg actgccgcta tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc
    25921 gcaatggacg tttactgcca ccgcctggcc aaatacatcg gtgcctacac tgcgctgatg
    25981 gatggtcgtc tggacgctgt tgtattcacc ggtggtatcg gtgaaaatgc cgcgatggtt
    26041 cgtgaactgt ctctgggcaa actgggcgtg ctgggctttg aagttgatca tgaacgcaac
    26101 ctggctgcac gtttcggcaa atctggtttc atcaacaaag aaggtacccg tcctgcggtg
    26161 gttatcccaa ccaacgaaga actggttatc gcgcaagacg cgagccgcct gactgcctga
    26221 tttcacaccg ccagctcagc tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg
    26281 aaagaggata aaccgtgtcc cgtattatta tgctgatccc taccggaacc agcgtcggtc
    26341 tgaccagcgt cagccttggc gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg
    26401 ttttcaaacc tatcgctcag ccgcgtaccg gtggcgatgc gcccgatcag actacgacta
    26461 tcgtgcgtgc gaactcttcc accacgacgg ccgctgaacc gctgaaaatg agctacgttg
    26521 aaggtctgct ttccagcaat cagaaagatg tgctgatgga agagatcatc gcgaactacc
    26581 acgctaacac caaagacgct gaagtcgttc tggttgaagg tctggtcccg acacgtaaac
    26641 accagtttgc ccagtctctg aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct
    26701 tcgttatgtc tcagggcact gacaccccgg aacagctgaa agagcgtatc gaactgaccc
    26761 gcaacagctt cggcggtgca aaaaacacca atattaccgg cgttatcgtt aacaaactga
    26821 acgctccggt tgatgaacag ggtcgtactc gcccggatct gtccgagatt tttgacgact
    26881 ccaccaaagc aaaagtgaac aacgttgatc cggcgaagct gcaagaatcc agccctctgc
    26941 cggttctcgg cgctgtgccg tggagctttg acctgatcgc gactcgtgcg atcgatatgg
    27001 ctcgccacct gaatgcgacc atcatcaacg aaggcgacat caatactcgc cgcgttaaat
    27061 ccgtcacttt ctgcgcacgc agcattccgc acatgctgga gcacttccgt gccggttctc
    27121 tgctggtgac ttccgcagac cgccctgacg tgctggttgc cgcttgcctg gctgccatga
    27181 acggcgtaga aatcggtgcc ctgctgctga ctggcggcta cgaaatggac gcgcgcattt
    27241 ctaaactgtg cgaacgtgct ttcgctaccg gcctgccggt atttatggtg aacaccaaca
    27301 cctggcagac ttctctgagc ctgcagagct tcaacctgga agttccggtt gacgatcacg
    27361 agcgtatcga gaaagttcag gaatacgttg ctaactacat caacgctgac tggatcgatt
    27421 ctctgactgc cacttctgag cgcagccgtc gtctgtctcc gccagcgttc cgttatcagc
    27481 tgactgaact tgcgcgcaaa gcgggcaaac gtatcgttct gccggaaggt gacgaaccgc
    27541 gtaccgttaa agcagccgct atctgtgctg aacgtggtat cgcaacttgc gtactgctgg
    27601 gtaatccagc agagatcaac cgtgttgcag cctctcaggg tgtagaactg ggtgcaggca
    27661 ttgaaatcgt tgatccagaa gtggttcgcg aaaactatgt tggtcgtctg gtcgaactgc
    27721 gtaagaacaa aggcatgacc gaaaccgttg cccgcgaaca gctggaagac aacgtggttc
    27781 tcggtacgct gatgctggaa caagatgaag ttgatggtct ggtttccggt gctgttcaca
    27841 ccaccgcaaa caccatccgt ccgccgctgc agctgatcaa aactgcaccg ggcagctccc
    27901 tggtatcttc cgtgttcttc atgctgttgc cggaacaggt ttacgtttac ggtgactgtg
    27961 cgatcaaccc ggatccgacc gcagaacagc tggcagaaat cgcgattcag tccgctgatt
    28021 ccgctgcggc cttcggtatc gaaccgcgcg ttgctatgct ctcctactcc accggtactt
    28081 ctggtgctgg tagcgacgta gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac
    28141 gtcctgatct gatgatcgac ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg
    28201 cgaaatccaa agcaccgaac tctccggttg caggtcgcgc taccgtgttc atcttcccgg
    28261 atctgaacac cggtaacacc acctacaaag cggtacagcg ttctgctgac ctgatctcta
    28321 tcggaccgat gctgcagggt atgcgcaagc cggttaacga cctgtcccgt ggcgcactgg
    28381 ttgatgatat cgtctacacc atcgcgctga ctgcgattca gtctgcacag cagcagtaat
    28441 ctcgtcatca tccgcagctt tgcgctgcgg atatctgaac cggaaatagt cactatttcc
    28501 ggttttttat tctcttaatt tgcattaatc ctttctgatt atcttgctta actgcgctgc
    28561 atcaatgaat tgcgccatct cactttgcat acttaccact ttgttttgtg caagggaata
    28621 tttgcgctat gtccgcaatc actgaatcta aaccaacaag aagatgggca atgcccgata
    28681 cgttggtgat tatctttttt gttgccattt taaccagcct tgccacctgg gtagttccgg
    28741 tgggcatgtt tgacagtcag gaagtgcagt atcaggttga tggtcaaaca aaaacacgca
    28801 aagtcgtaga tccacactca tttcgcattc tgactaacga agcaggcgaa cctgagtatc
    28861 accgcgtaca gctgttcacg acgggcgatg aacgcccggg tctgatgaac ttcccgtttg
    28921 aagggttaac ctcaggatcg aaatacggga cagccgttgg catcatcatg tttatgctgg
    28981 tgattggcgg cgcgtttggc attgtgatgc gtacaggaac cattgataac ggtatcctgg
    29041 cgcttattcg ccatacccgc ggaaatgaaa ttctctttat tcctgcgctg tttattctgt
    29101 tttcacttgg cggcgcaata tttggtatgg gagaagaggc cgtcgccttt gccattatca
    29161 tcgcaccgct aatggtccgg ctgggctatg acagtattac caccgtcctt gtgacctata
    29221 ttgccacgca aatcggtttt gccagttcgt ggatgaaccc gttttgtgtg gttgttgctc
    29281 agggtattgc cggcgttccg gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta
    29341 tcgccactct gattggcctg atctttacca tggtgtacgc ctcacgagtg aaaaagaatc
    29401 ctcttctgtc acgcgtgcat gagtccgacc gcttctttcg tgaaaagcag gctgatgttg
    29461 aacaacgtcc gtttaccttt ggtgactggc tggtattgat tgtcctgacc gccgtaatgg
    29521 tctgggttat ttggggcgtg atcgttaatg cctggtttat tcctgaaatt gccagccagt
    29581 tcttcaccat gggtctggtg attggcatca tcggcgttgt tttccgcctt aacggcatga
    29641 cggttaatac catggcttca tcctttaccg aaggggcgcg aatgatgatc gcccctgccc
    29701 tgctggtggg tttcgccaaa gggattttgc tgctggtcgg taatggtgaa gcgggtgatg
    29761 ccagcgtgtt aaataccatc ctcaacagca ttgccaatgc cattagcggt ctggataacg
    29821 cagtcgcggc ctggtttatg ttgctcttcc aggcggtatt taatttcttc gtgacgtccg
    29881 gttctggtca ggcggcgtta accatgccgt tactggcacc gcttggcgat ctggtcggtg
    29941 ttaaccgtca ggttaccgtg ctggctttcc agtttggcga tggcttcagc cacatcattt
    30001 acccaacctc agcttcgtta atggcaacgc tcggtgtttg ccgggtggac ttccgtaact
    30061 ggctgaaggt gggtgcgacc ctgcttggac tgctgtttat tatgtccagc gtcgtggtga
    30121 tcggcgctca gttgatgggc taccactaaa aatgttaaga gccgcgattg cggctctttt
    30181 tttcattctg ccgtttcagt ctctacggct tcatttttgg cattgcgctt catccacaac
    30241 gccagcgctt tcagcgagtc tggagtgaac tcatcgcagc gtgcggtgat ttcttccggc
    30301 gtcagccagc aaacttcact gacttcatct tcctgtagag cgaagggacc gtgagagacg
    30361 cagctgaaca acgcgcccca gacacggcaa tttttatctt cgaaatagaa ctgcccgtgc
    30421 tcggcaaagg ggaccccggc aatgcccaac tcttcttccg cttcgcgacg cgcggattcc
    30481 agcagttgct catcggcctg gactacaccg cctgcggtcg catctaacat gccgggtaaa
    30541 aagtcttttg tctcggtacg acgctggacc agaattttgc ccatgccatc atgcacgacg
    30601 atataagttg cacgatgacg cagacactgt gcccgcattt gttcccggct ggcttgtgca
    30661 atgacttcgt tctcttcatt gacaatatcc acccattcag tacttgccaa acgacgctgt
    30721 tccattatca ggaaaccttc tttttctggc gctcttacgg cgcattttga gttgtgggta
    30781 aattacggat taatcgcgac ctgcgcaatg atactttgat cattgagtgc gattacacta
    30841 agtacgtcat tatccagcat gccataactc gccggattac cgcctttcgg aatactcacc
    30901 gagccggggt tgaagtggaa aatctccccc cgctgttctg ccactggcag atgggtatga
    30961 ccgtacacca gcacatcgtt ctggtttaaa gcaggtagat tttccgggcc aaaaagatga
    31021 ccatgcgtca aaaacagacg ttgtttttcc agtaatacct gttgccacgg cgcggttatc
    31081 gggaaatgca gcagcatttg atccacttcg ctgtcgcagt tgccgcgcac agcgataacc
    31141 ttatgtgcca cttcattaag ccgttcagcg actttggctg gcgcgtaacc ctccggtaaa
    31201 gcattacgcg ggccatgatt caacacgtcg ccaaggatca ccagccactg ggcaccgctt
    31261 tggacaaaca actccagaac acgttccgtc gccggtaacg acccatgaat gtccgatgca
    31321 aacatcagtt tcatcactca ctcctcgtcg aaaaaacgta agctatgata ctggattctg
    31381 tcgttgctat cagccagatt gcttcgccga aagtgcaata aaacgctgga cagacgctcg
    31441 ctgccactgg aatgtcgcat aatccaccag gcgttccggc acctcatcgc catgtagtac
    31501 caggcggtta atcattagcg ccagatcagt atcagcaatg caccattcac caaataaatt
    31561 cggctgaccc agtgctaaca aatgctctgc catcgcgaac aatttctctg cactggcttt
    31621 tccctcggcc gttagtggcg cttttttcgc ccccgcaaag acaacatccg tcgggcgttc
    31681 ttcgcgaatg ggcatcagat cgctgcgcaa ccaagcctga atctgtcgtg cacgcgcacg
    31741 attttctaaa tcaagcggat aaatacgttc ccaggtcggt ggcgcaaatc gatcttccag
    31801 atactccgca atggcagaag attcactcaa ctcaaaatca tcgatttgta ataacggcac
    31861 acggcgtgtc tgaccgtaac cttgccacgc cggctgcaaa tgttcaccgc tgtcgaggtc
    31921 gatggtcttg atatgaaacg acaggccttt ctcctgcaac gccacccagg cggataacac
    31981 ataaggggag aaaaaatggg catctgacca aagcgtgata gcggttttac tcattatgtc
    32041 ctcggcaata aagggggctt gctatcaaac tatagagcct ttttgtcact gtcacgtgac
    32101 gaatactcac gcaagtcgcc gcgctttcta tacttgtggt gttcagatga ttagacaacc
    32161 ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca atggtcacaa aattacgctg
    32221 tttctcgaag aagcagggct ggattatcgc ttaataaagg tagacctggg gaaaggaggt
    32281 cagtttcgcc cggaattttt gcgcatttcg cctaacaaca aaattccggc aattgttgat
    32341 cattctccag ccgatggcgg cgaaccgcta agcctctttg agtctggtgc cattttgttg
    32401 tatctggctg agaaaacggg actctttttg agtcatgaaa cgcgtgaacg cgccgtcaca
    32461 ttacagtggt tattctggca ggtaggcgga ctggggccga tgcttgggca aaatcatcat
    32521 tttaatcacg cagcccccca aaccattcct tacgctattg aacgttatca ggttgaaact
    32581 cagcgtctgt accatgtact gaacaagcgg ctggaaaatt cgccctggct gggaggcgag
    32641 aactacagca ttgcggatat tgcctgctgg ccgtgggtta atgcctggac tcgccagcga
    32701 attgacctcg caatgtatcc ggcagtcaag aactggcatg agcggatccg ttcgcgccct
    32761 gccaccgggc aggcactgct aaaagcacaa cacggtgatg agcgttcgga tagttaacag
    32821 aaacaggttc tcgtgtatta tttcattcta agtaaaacaa cggagaacct gcaatggcac
    32881 aacctgccgc tattattcgt ataaagaacc ttcgtttacg tacgtttatc ggaattaagg
    32941 aagaagaaat taacaaccgt caggatattg ttatcaatgt gacgatccac taccccgccg
    33001 ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa ttatcgcacc gtaacgaaaa
    33061 acattattca gcatgtagag aataaccgtt tctctttgct ggaaaaatta actcaggatg
    33121 tgctcgatat cgcacgtgaa catcactggg tgacgtatgc tgaagtggag atcgataaac
    33181 tgcacgcgct gcgctacgcc gattcggtat ccatgacctt aagctggcag cgttaaccgc
    33241 catatcggga ggctgcatga acatagtgat caccggaggg acgggattaa ttggtcgcca
    33301 tttgattcca cgtttgctgg acctgggcca tcaaatcacg gtagtgacgc gtaacccgca
    33361 gaaagccagt tccgttctcg gccctcgggt gacactatgg caagggcttg ccgaacaaag
    33421 caacctcaac ggcgttgatg cggtaatcaa cctggccgga gaaccgattg ctgataaacg
    33481 ctggactcac gagcaaaaag agcgtctctg ccaaagccgc tggaatatca cgcaaaaact
    33541 ggtcgatttg attaatgcca gcgacacgcc accgtcggta ctcatttccg gctcggcaac
    33601 gggctattat ggcgacttag gtgaagtggt ggttaccgaa gaggaaccgc cgcataacga
    33661 atttacccat aaactctgcg cccgctggga agaaattgcc tgccgggcac aaagtgacaa
    33721 aacgcgagtg tgcctgctgc gtaccggcgt agtgctggca ccggatggcg gtattctcgg
    33781 taaaatgctg ccgccgtttc gtcttggcct gggcgggccg attggttccg gtcggcagta
    33841 tctggcttgg attcatattg atgatatggt caacggcatt ctctggctgc tggataacga
    33901 gctgcgcggg ccatttaata tggtttcgcc ctacccggtg cgcaatgaac aatttgccca
    33961 tgcactgggt catgcgctgc atcgcccggc cattttgcgc gtccctgcga ccgccattcg
    34021 gttgttaatg ggcgaatctt cagttctggt attaggcggt caacgcgcgc tgccaaaaaa
    34081 gctggaagaa gcgggttttg cgtttcgctg gtacgattta gaagaggcgc tggcggatgt
    34141 cgttcgctga tgtggtttac tgcaaacatc cgccagttaa ctcccggtgt tacagggtta
    34201 gtggctttgc gcgataagat cgtctggtga aagtcgggtc agcgtcagga tgacttcgtg
    34261 gtcaaatcct ttttccatca tctcctgagc aatgcgcagg gcttcttcgt gtttgccctg
    34321 cattgcgcct tcttcacgta atctgtcagc aatggtcatc agtttctcct tttcttgtgg
    34381 tgcgcgttcc gctatctcac caataaatgc acggaaacgc cgggcatccc ccgtttgtaa
    34441 tacgtaatta aacagggctt ttagctgtct gtcattagtg ttccctgtaa ctagcagcga
    34501 aacaatttgg tcgactaatc ccaacagatc gcgctgacga atatgtttct gaattaactc
    34561 caacagcgcc attttgcgat gttgcataat ctcgtcatcc ggcaccacgg taatatccac
    34621 cagcggaaaa gccgatgaat aaattttgcg ggcaatcgca ggctcggcaa attcatcaag
    34681 ccagcagagt gaatatggat aagggcttct gcaaccatga taaaacagca tcgggatcac
    34741 taatggaagc tctttataac ccgcatcaag atggttttgc atcgccgcaa tggaatagcg
    34801 catcatgcga aaagccatta attcctctgg cttactttgg tgctctatca ccacataaat
    34861 ataacccgct ccctcctgcg ttttcacaga ccacaagagg tcggaataat attgccgcag
    34921 gtcttcatca ataaaactgt ttggttccag tttaagcgtc gttaaatcac acagtttgcg
    34981 cagcggcgcg ggaagatgaa tatcaataaa atcccgcgcg gtgtctggat ggcgtaaaaa
    35041 agatttaaat accgcatcat gcggcgtgga agttgtcgat attgtcatgg cgttccgtca
    35101 cccctcaaat ttaagatgac gcgacaataa ccacgattta cacccaatgc agcaggcagt
    35161 ttttatcttt ggacagcgaa ttcagaacta tttttagcgg actacaactt ttacatttca
    35221 gcggaagcgg actcgcaaat tccccatgaa atactccggc agcgttaacc accggagagg
    35281 aaattatttc agcgaacctt taaggaactg ttgcagacga gggctttgcg ggttaccgaa
    35341 taactgctcc ggcgcccctt cttcttctat tttcccctga tggaggaaaa tgacgtgagt
    35401 agaaacatga cgagcaaagc ccatttcgtg agtcactacc accatggttt tcccctcttc
    35461 tgccagttgc tgcataatac gcaacacttc gcctaccagt tcaggatcga gcgctgaagt
    35521 aggttcatca aacagtaaaa cttccggttc catcgctagc gcccgcgcga tagaaacacg
    35581 ctgttgctga ccaccggaca ggtgtaccgg gtatttcccc tgcgcacgtt cgtctatccc
    35641 gacttttgcc agatatttca ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac
    35701 ctgaatcggc gcttccatga cgttttccag caccgtcata tggctccaga gattgaagtg
    35761 ctggaatacc atcgtcaggc gtgtgcgcaa taagcgcagc tgatttttat cggcgacttt
    35821 gagttgaccg tctttatcgc gcaccagatt gatcgtctgg ccattgacca cgatcgatcc
    35881 ttcgctcggt ttttcgagga agttaatgca gcgcagaaag gtacttttac ccgatcccga
    35941 cgatccgatg atgcttatga catctccggc attcgcttgc aatgagaccc ctttcagcac
    36001 ttcgtgttcg ccgtagcgtt tgtgcaaatc gataacgttt aatttattct cggacatcgt
    36061 attctcagtg cgttgaagaa ggtttcacat gctgcaacca gcgtttttca gctctacgaa
    36121 agaggctgat caggacataa gagatgatta aatagagcac cgcggcaatg ccgaaagcgg
    36181 taaaaggttg atatgtggcg gcgttaatat cgcgggcaat tttcagcaga tccggcaccg
    36241 tggcagtaaa tgccagcgca gtagagtgca gcatcaggat cacttcgttg ctgtacgccg
    36301 gtaacgcaat acgcagcgca gaaggcaaaa taatgcagcg atacatttta aaggtcgaga
    36361 agccataggc tctggcggct tcaatttccc catgcggcac tgaacggatt gccccggcaa
    36421 aaatctcggt agtgtaagcg caggtgttaa gcgtcagcgc cagcacggta cagttcagac
    36481 cactgcgaaa aaaggcgtta aggaactcgg ttcccttaac aatctccagc gtgtacatgc
    36541 cggaatagaa caccagcaac tgaacataca gcggcgtacc gcgaaaaata taggtaaata
    36601 accagattgg aaactggatg tatttattac ttgagacgcg accaatcgcc agaaacagcg
    36661 ccagaactcc gcctatcact accgacaaaa tcagcagcca cagggtgatc gccacgccag
    36721 taaagcgata accgtcagtc cacagcagtg gtttccagta ttcatgtaag atttcgatca
    36781 caggtcagcc ctcttcacac ccacggagta gcggcgctca aggaacagca gcacaccatt
    36841 ggaaacggtg gtgaaaacaa gataaatcac gccgcagacg atagcgaaat agaacggttc
    36901 ccaggtactt ttgcctgcca gctgcgtggc tttgaccaca tcttccaggc cgagtaacga
    36961 aaccagtgcg gtagatttga ggatcacctg ccagttgttg ccaatgcctg gcagggcgta
    37021 acgcatcatc gccggaaaca tgatccgccg aaacacttgc tcacgagtaa aaccgaacgc
    37081 cgtcgccgcc tctatatgtc ctttcggcac tgccataaac gcgccacgaa acgtttcggt
    37141 aaaataagca ccgtagataa aaccgagagt gataatacct gcgaccatcg gatcaatatc
    37201 aatctgcccg acgcccatcg cctccgtcac cgtgtttagc gcaatctgca aaccgtagaa
    37261 aatcagcagc atcaatacta aatccggcac gcctcgaatc agcgtggtgt acccttcgaa
    37321 aataaggccc gaaagccgat tttgtgagag cttaccgcca gcgccaatta aaccgatgat
    37381 tacagcgagc actacagagc tgatagccag ctccagcgtg acgagcgcac cctgtaaaat
    37441 aacacctgaa aacccataca acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct
    37501 cttgtctccc tccggatacc cgaagggagc gggctttctt tcaccgatgg cgattaacca
    37561 ccataaacat caaaatcgaa gtactttttc gctaatttct cgtaagtacc gtcagcgcgc
    37621 atttcggcaa aggctttgtt cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc
    37681 atgccggtcc ctacgccaaa cagtttttca tctttaacag acgggccacc gaatttgtaa
    37741 tctttaccga cgggttgttt gaggaaacct tcgctggctg cgacctcatc ctggaacgcg
    37801 gcatcaatac gtccggcagt caggtcagaa taaatattgt cctgcccctg ataagaaacg
    37861 atttcaatgc cttttggtgc ccagtgttcg ttaccgaacg tctcctgggt ggtgccctgc
    37921 aatacgccta tccgtttgcc tttcagcgat tcgactgtcg gctgaatgtc ggaatttttc
    37981 gccaccacca aacgagaatc ggcagcgtac agtttgtcgg tgaaggctat ttcttgctga
    38041 cgtttttccg taatggaaag cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac
    38101 gggattaacg catcgagtgg gttttcgaca aacgtacatt gcgtattgat gcgtttgcac
    38161 aactccttcg ccagatcgat atcgaagcca accagttcac cttgtgaatt ttttgattca
    38221 aatggcgcat aggtcggatc ggtaccgatg cggatgtttt gcggaatcgc agcaaatgcc
    38281 gcagttgcgc tggagaaggc cagaaccaga gagagcgata gcaccagttt tttcataatg
    38341 tcctcaactg acagtctttt tatcgggaat atttacaggt ttgttacact tatcgtgcca
    38401 taaaattgac catcaaggca aaatatcccg ccccgctaga cgtattattt ttcattaaaa
    38461 atgcttaagt atgcatttca atgcaccacg atagtgcaac aaacctggcg tgcaccatga
    38521 tgatcattcc atcaggtaca gcttccctgc gacgtatcag tcaccgtaga cattaaagtc
    38581 gaaatacttt ttcgccatct tgtcgtaggt accgtcctga cgcagctcgc caagcgcctt
    38641 attaaacgca gccgtcagtt cagcatcatc tttacgcagc cctacaccgg tgccgtcacc
    38701 gaagtatttt ttgtctttca ctgatgggcc tgcaaaggcg aaatctttac cggcgggttg
    38761 cttaaggaaa ccttcgctgg cagcaacttc atcttgtaac gcagcatcca gacgtcctgc
    38821 agccagatcg gaatagacta aatcctggtt ggcataggcc accacatcca cgcctttgct
    38881 acgccaggtc tcgttagcgt atgcttcctg ggttgatccc tgcagcacac caacatgttt
    38941 acctttcagt gaatccagcg ttggctgaat cggtgaacct ttggcggcaa tcaaacgaga
    39001 atctgcggcg tacagcttgt cagagaaggc tatctcctgc tgacgtttat cggtaatgga
    39061 gagcgacgaa ataatggcgt cgattttttt cgctttcagt gaggggatca gcgcgtcaaa
    39121 gtcactggca acccaggtac atttcacctg catccgtttg cacatctcgt taccgagatc
    39181 gatatcaaag ccaacaaagt cacctttggc atctttcgat gagaacggtg cgtaggtggt
    39241 atcggttccg atacgtaccg tctccggtag cgccgcatag ctggaagccg ctgcggagag
    39301 accgactaac aaagacagag cgagaatcga cttcttcata cataaccctc aagtgaaaat
    39361 ggctttttta tgttgtgtat tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg
    39421 ccatcttgac gacagcagta acattcaacg ttaaatatgt taataagacg ttgcattatt
    39481 gtcctaaagt tgaagatagc aggtatagcg gttgaatcgc agcgtttcga ttgaatggca
    39541 gaaacaaaat gtcgaggatt tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc
    39601 ggagttatgc gccctgccag cgggcaaaga gatcttcagg aagggttacc gcaaactggt
    39661 caagaacacg attaaccgtc tgatttatca catcatcaag ggattgtggg cgatgataaa
    39721 acgccggaac gggaggcata atcaccgcac cgatttctgc cgcctgagtc attaaacgca
    39781 gatggcctaa gtgcagtggc gtttcacgca cgcagagcac caacgggcga cgctctttca
    39841 gcaccacatc tgccgcacgg gtcagtaagc catcagtata gctatggaca atgccggaca
    39901 gggttttgat tgaacagggt aaaatcacca tccccaacgt ctggaaagaa ccggaagaaa
    39961 tgctggcagc aatatcgcgc gcatcgtgtg tcacatcagc taacgcctgc acttcgcgca
    40021 gggaaaaatc cgtttcgagg gataaggtct ggcgcgccgc ctggctcatt accagatgcg
    40081 tttcgatatc tgtgacatcg cgcagaacct gtaataaacg cacgccataa atcgcgccgc
    40141 tggcaccgct gatgcctaca atgagtcgtt tcataaatag ttgccctgtc agacttgcag
    40201 gcagactttg caggatttcg gcgggagttg caagtcaggg tgccagaccg gcaccctgag
    40261 tgaaggcatc atccttcgtt atgcatttcg agattttcca cttcgttctg acgttgcacg
    40321 gctttggcgt cgtcattacg taaagtatcg aggaaatcga ggtagccctg atcaacgtct
    40381 ttggtgacgt agacgccgtt gaacaccgag cattcaaact gctggatatc cgggttttca
    40441 gcgcgaacgg cttcgatcag atcgttcaga tcctggaaaa tcaacccgtc agcaccgatg
    40501 atctggcgaa tttcatctac ttcgcgaccg tgagcgatca gttccgtggc gctcggcata
    40561 tcgataccgt aaacgttcgg gaagcgaatt tccggtgccg cagaagcgag gtacactttc
    40621 ttcgctccgg cttcgcgtgc catctcgata atctgctcag acgtggtgcc acggacgata
    40681 gagtcgtcga ccagcaggac gtttttatcg cggaactcgg cgcggttggc gttcagttta
    40741 cggcgcacgg acttacgacg cagctgctga cccggcatga tgaaggtgcg gccaacatag
    40801 cggtttttaa cgaagccctg gcggtacggt ttgcccagaa tacgcgcaat ttccagcgcg
    40861 atatcacacg aggtttccgg aatagggatc accacgtcga tatccagatc ttcccattcg
    40921 cgggcaattt tttcgcccag tttggtgccc atattcacgc gcgcgctgta aacggaaatt
    40981 ttgtcgatga acgagtccgg gcgggcaaag tatacatact caaacaggca cggattgctg
    41041 accggattgt cagcacattg acgggtaaac aactgtcctt cttcagtgat gtaaatcgct
    41101 tcgcccggcg cgacgtcacg caggaaatca aagcccagcg tatcgagcgc aacgctttcg
    41161 gaagcgacca tatattctgt gcggttctcg tcaatatcac gttttcccag taccagcgga
    41221 cgaatcccgt taggatcgcg gaaagcaacc ataccgtggc cgatgatcat cgccacacag
    41281 gcatacgcgc cgcggattaa gcggtttgta gcagcgatgg cggcgaaaat gttgtcggct
    41341 tccagcgggt agtggcggaa gttgtccagc tcgctggcga agatattaag cagaatttcc
    41401 gagtcggaag tggtgttgat gtggcggcgt ttttcttcaa acagtttttt acgcaactcg
    41461 tgagcgttgg tcagattgcc gttgtgggca agcgtaatgc catacgggga gttaacgtaa
    41521 aacggctgcg cttcagaggc gctggagctg ccagccgtgg ggtaacgcac atgaccaatg
    41581 cccatattgc cctgcaaacg ctgcatatgg cgagcttcaa atacatcgct caccagcccg
    41641 ttcgctttcc gcaaacggaa gcagttattg gcatctatgg tgatgatgcc ggcggcatcc
    41701 tgaccgcgat gctgaagcac cgttaaggca tcataaatcg actggttaac cggcataaca
    41761 ccggcgatac cgacaatacc gcacatacgt ctttttcctc gttaagccac atctcagagc
    41821 acttacgctc tgggcaagaa acttgacgag ctttgcagat aatcaaaaaa ccatctgatg
    41881 ataaaactga actgcgggat cagctgtgat ttgctccagt cttcgctttt cgacaccccg
    41941 gtaaaggagt cgagaaagaa gagaatggca gcaacaatca acacaccgcg caacgcaccg
    42001 aaacagacgc ccaacacccg atcggtgcct gacaaccccg ttttctccac caactggcct
    42061 atcacgaagt tcacgatagc accaacgatc agggtagcga taaacagtac cgcgatggca
    42121 atcccatttc gaaccagttc gtcttcaaag cccgtaaacc agactgacag gtaagtgtag
    42181 taatgactgg caacaaagaa agcacaaccc catgtcacca gcgataacgc ttcacgaaca
    42241 aagccgcgga tcaggctaac cagagaggaa aaagcaatca ccgcgattat ggcgtaatca
    42301 atccagacca tatgtgtccc acgattttac gccctgtcat cctgttcggg gcgcattcta
    42361 acagaaaaag aaaacgtttg cgtagggatt tccttcccgc gcatcaataa aaatggcgct
    42421 gaaaaaatat tcaacgccat cgacttttta tgcctttgcg gcatcgggca atgcgtgtcg
    42481 gatgccgcat gaacgcctta tccgacctac agttctgctc cagcacaggc ctgataagac
    42541 gcgctggcgt cacatcaggc aacggctgtc ggatgcagcg tgaacgcctt atccgaccta
    42601 ctgttctact cctgcgttgg cctgataaga cgcgctagcg tcgcatcagg caacggctgt
    42661 cggatgcagc gtgaacgcct tatccgacct actattctac ccctgcgtag gcctgataag
    42721 acgcgttggc gtcgcatcag gcaacggctg tcggatgcgg cgtgaacgcc ttatccgacc
    42781 tacggttcta cccctgcaca ggcctgataa gacgcgctgg cgtcacatca ggcaagaccg
    42841 tattaattcg gtgtatagcc cattaccacg ccacttaagc cagaaagctg cttgagctca
    42901 cccagcgatc ctttcagctt ctctttggaa gcatccggcc caaccagaat acgggtaatt
    42961 ttaccctgca ctggcgtgga tggcgacgta taaacccgat aaccggcacc gcgcagctta
    43021 ccgacaatct cattcacttt atcggcattt ttcagcgcgc ccagttgcac aacataagct
    43081 ttacccgttg gcgctgcttt ttcttccacg accggcttcg gttctggctt cggcgcaggt
    43141 ggtgcttcaa cctttggctt aagcggctcc accggtttcg gcttcggtgg ggcgaccggt
    43201 gcaggttccg gttcaaactc ggtgttattg gctgcaatag tggccggatc gagcgacggt
    43261 gctgcggcat cacctgcccg tacctcttcc gctgcgcctt ccggcggctg cgtcggcaac
    43321 gcctgggtgg cggctggcat catatcaggc tcatcgcgat cgcccgcttt tggcaccagc
    43381 gggatagccg cgaactcatc ctgataatgt tttttctgcc cgtccagcag ccctggaagt
    43441 acaatcaccc ccagcgctac cagcacgatc gtgcccacta accgattctg aaacttactt
    43501 gccaccgctt ctcctcgcgt caatcacttc catgacatgt gcgaccgtgt ggaacgaacc
    43561 acacaccagc acggtgtctt ccgctttagc gtccgccatt gcggcatccc atgcctgcgc
    43621 aacgctatca aatgatttgc cgttacccaa atgctcaagc agttgttctg ccgtggcacc
    43681 gcgcggccct tccagtggcg cacaatacca gtcatcaacc acgcttttca accaggccag
    43741 agttccggca atatctttat catgtagcat accgataacc gccagcacgc gcccgttttt
    43801 cggtagcgct ttcatacgcc cggtgagata ttccgccgca tgtggattat gcgcgacatc
    43861 aaaaataacg cgtggcgact cgctcacaat ctggaaacgt cccggcaaaa ttgcgctggc
    43921 aatcccgtcg cgaatggcat tttcactgac ctccagcccg ctggcacgca gtgccgccag
    43981 cgccgttgcg gcattcggtt gcgggacaag cggcaacggc agattttcca gcgtgccgtg
    44041 agcatcgcta aacgtccagt catgatcggt gacggaatag ttccactcaa cgccccgacg
    44101 ttgtagcagt gcaccttttt cctgcgccac atcagcaatg gtagaaggca tctccggctc
    44161 accgacaatt gccggttttt cgctgcgaaa gatacctgct ttctcgcgac caatactttc
    44221 acgatctggc cccagccagt cggtatgatc cagcgcaata ctggttacta ccgcgacatc
    44281 ggcatcgaca atattggttg cgtccagacg accgcccagc cctacttcca gaatcactac
    44341 gtcaagttgt gcctgcttga acagccacaa cgccgacagc gtaccgtatt cgaaataggt
    44401 cagggaaata tcaccgcgtg ccgattcaat ctccgcaaaa gaagcggtgt gggccgattc
    44461 cggcaattcc tgaccctgca cacgtacgcg ctcggtataa cgtaccagat gcggcgaact
    44521 gtagacgccc actttgtacc ctgccgccat cagaatcgac tccagtgtac ggcaggtggt
    44581 gcctttgcca ttcgtacccg caacggtaaa cacaaatggc gctggtttca gaacgccaag
    44641 acgcgccgcg acctggctca cgcgctcaag gccgagatcg atagttttac tgtgtaggtt
    44701 ttccagataa gaaagccacg aagccagagg cgacgcggct tgaggagtgc gtttgataat
    44761 catggtatcc gctgattcgt tacggtgaga atagcaaaag ggcagagcca gtggccctgc
    44821 ccttatcagt tatcaggcct caggttcctg atccggtacc ggtggcacca ctacgccttc
    44881 acgcggcgct tcaggattcg gcgctggcag attcatcaac ttcgccagaa tgctcgccag
    44941 tttcaggcgc atttccggac gacggatgat catgtcgatc gcgcccttct cgatcaggaa
    45001 ttcactgcgc tggaatcctg gcggcagttt ttcgcgaacg gtctgttcga taactcgcgg
    45061 accggcaaag ccaattaacg ctttcggttc agcgatgttg agatcgccca gcatggcgaa
    45121 acttgcagaa acaccgccca tggtcgggtc ggtcagcacg gagatgtacg gcaagccgcg
    45181 ctcctgcatt tttgccagtg ccgcagaggt tttcgccatc tgcatcagcg acatcagtgc
    45241 ttcctgcata cgtgcgccac cagaggcgga gaaacagatc agtgggcagt tatcttccag
    45301 cgcctgctca acggcacgca cgaaacgcgc acctaccaca gaccccatgg aaccgcccat
    45361 aaaggcgaac tcgaatgccg cagcgacaac cggcattcca tacagagtgc ctttcatcac
    45421 caccagcgca tctttttcgc cggtttcttt ctgcgcagat gccagacggt ctttatactt
    45481 cttggagtca cgaaacttca gcacatcttt cggctcaagc tcgctaccca gctccacaag
    45541 gcttccttca tctaacaggc tatgcaggcg attacgcgct gtcatacgca tgtgatggtc
    45601 acacttcgga cagacctcaa gattacgttc cagctcagcg cggtataaaa cctgaccgca
    45661 gctatcacac ttagtccaca ccccttcagg aatgctcgcc ttgcgggtgg gagtaatgtt
    45721 gcttttaatt cgttcaatcc agctcattag ggacctttct gtctgaacct ggttcgatgc
    45781 cagttttatc tttggggacg cataatgcca tttttgcccc caacagacca tgaatgttgc
    45841 acattaaaac ataacagccc gaaactttgg ataaaaaagt ggtcgaaccg cagagttact
    45901 ttttattttg cggcgcgtgc cgctgcgcgt ttgtggcgaa taaattcgaa aacacctggc
    45961 aatattgaaa caaaaataat gccaacaatc atcagtttaa ggttgctctg aatgaagggg
    46021 agcgtgccga agaaatagcc tgcgtaggta aaaagcagta cccacaacag tgcgccaatc
    46081 acgttataag cggcgaaatg acggtacgac atgtggccca ttcccgcaac aaacggggcg
    46141 aacgttctga cgatcggcac aaaacgggcg agaataatcg ttttgccgcc atgtttctca
    46201 taaaactgat gggttttgtc gagataactg cgacggaaaa ttttcgaatt gggattactg
    46261 aataactttt caccgaacaa ccgcccgata gtgtaattga ccgcgtcacc cacaatcgcg
    46321 gcgatcagca tcagcaccac catcatatgg acgttgagat cgttggtctc cagtgatgcc
    46381 aacgctccgg caacaaacag caatgaatca cccggtaaaa acggcgttac caccagaccg
    46441 gtttcacaga acaaaattaa gaacaaaatg gcataaaccc atacgccgta ctccgcgacc
    46501 agctctgcca gatgcacgtc aatgtgcagg ataaaatcga tgaggaaata aatcaggtcc
    46561 atatttgcct atgccttgta ctcgtcatct atcaggctgt aactacgttg gctacagcct
    46621 gaaatgttcc gagcattatt ctcgttagtc cgccagaaat agcgggccca ttggcggttt
    46681 tggaagatca tatcggtcag ggtaatccac cgcgaccaga tatagccctt ccgcttttgc
    46741 cgttgctgcc gccagcgttc tgtcctttgc cgccagcaat tctgctatcc agctctccgg
    46801 ctggttgtgg gcaccgactt ccatcaggct gccgacaata ttcctgacca tatgatgtac
    46861 aaaggcattc gctttgatat ctaccaccac ataaggaccg tgacgcgtga cattaatatg
    46921 cataacgttg cgccacgggg tccgggactg gcactgcacc gcacggaacg aggtgaaatc
    46981 attctcgccc agcaagcatt gcgcagcccg atgcatccgt tcagcgtcca gcggttcgta
    47041 aaaatgggtt acccctttac tcaataccgc cgggcgcagc cgatgattgt agatgatgta
    47101 gcgataacgg cgagccgtgg cgctaaatcg ggcatgaaaa tcatcaggta cagctttaac
    47161 ccaacgcacg gcgatgtcac caggtaaatt cgcatttacg cctaacgtcc acgcagcgtc
    47221 tttgcgctgc gcggtcgttt cgaaatgcac cacctgcccg gtaccgtgta cccctgcgtc
    47281 agtacgcccg gcgcagaaga cggtgatggg ttcgttcgcc acctgggaga gcgccttttc
    47341 cagcttctcc tgcacactgc ggacttcatt ctgccgttgc cagccgtaat acctactgcc
    47401 gtcgtactca atgcccagcg caattttata aactggcggt tgttgctggt cggacattag
    47461 tacagatact cctgcaccag tttctcggcg atttttactg ccatcagcgc gccgccaaag
    47521 cgaacgttat cggccaccga ccagaactgg acttgctccg gcataccgta gtcattacgt
    47581 acgcagccaa cagaaagatg cggacaacca gaagcatctc ctacctgagt cgggaattcg
    47641 ttctcttcag agagcacaat atcttcgccc tgagcaaacg catcacgcgc ttcttctgcc
    47701 gccagcggac gcagtgcttc aaagttgacc atctgggcat gaccgtagaa taccggtgcc
    47761 tggacgacgc ttgccgaaat catcacccct tcgtcctgca ggattttgcg tacttcgtca
    47821 acgatacgac gttcttcacg cacgctacct tcgctatccg gcagtaacgg cagcatgttg
    47881 aacgccagct gacgcccgaa gaaatcttct tcgtcaatcg gaatgccatt gagtaattta
    47941 gcactctgcc ccgctaacgc atcgaccgct tttttaccct gggcggaggc tgaaatcagg
    48001 ctggtaacgc tgatacgtga taaaccgccc tgatcaatta gcggtttcag tgccgccagc
    48061 agctggctgg tcagactgtc tggtacggcg atgacattcc ggttgcggta atcggtcagt
    48121 acaaacgggt ttacttccgg caccaccagc ggtacgtcgg gttcgagagc aaacaatccg
    48181 ctgctgtcga tcaccagaca acctgagttg gttgcttctt caacccaggt agcggtagct
    48241 tctttgcctg cgacaaaaaa cgccagctgc gcctgcgtcc agtcgaattc agcggcatcc
    48301 tgcacggtga ttgtcttacc accaaagcgc agttgttctc ctgcgctttc gttacgtgcc
    48361 agtgcataaa tttccccaac cgggaactga cgttcagcca gcgtttcaag cagggcttcg
    48421 cccacagcgc cagttgcgcc caggacggca atgttccagc cttcagacat ggtggtttac
    48481 tccagaaata gcaaagctcc ctgccaatgt tacagcagag agcatgaaga agagattaac
    48541 gtgccggatg atgaacggcg ttaaaaccca gtttacacag caatgatgcc gcactggcgt
    48601 catcacaaat cacatacaga gatgaccatt cacggcgctc aagatagttt ttgcgcagtt
    48661 tatcgaactc acccggtatc ccggcgactt tacgcagcgg tgcgtcatcg cggcgcacat
    48721 catacaccaa atgcaccagc cttttcagcg tcggttgatc gagcgggcca tgcagcgtaa
    48781 tgcgaccaaa ctctggcgcg ggcagtaatg tatccagcgc aacgtgctgt tcatgcccaa
    48841 taaacttgct ataagcttca aacacttgcg tagtaccgcg tgctttacct tccagggtat
    48901 agcctgcgat atgcggcgtg ccgatatcca cttttttcag caattccacg ttaagttccg
    48961 gttcgccttc ccatacatcc agcactacgc ttaacttctg gccttcgctc aggcaggtca
    49021 gcagggcggt attatcgacg actgcgccac ggcaggcgtt aatcaggatc gctcccggtt
    49081 tcaggctgcg gatcagtttt tcatccgcca gatgcagcgt tttgtacgga ccatctttaa
    49141 agagtggcgt atggaaagtc agaatatccg catgctggac taactcatcc agcgagcgga
    49201 aatcaccctc atccccacgg tcggcgcggg gcggatcgca aagtaatgtt ttgatcccta
    49261 acgcttccag tcgcgcctgt aaacgacgac caacgttacc aacgcccacg atccccacgg
    49321 tacgctcgtg cagtgaaaat ccatcgcgtt cggcaagcat cagcagggag gagaaaacat
    49381 attccactac cgcaatcgca ttacagccag gtgcagcgga aaaaccaatt cccgcctgct
    49441 ttaaccatgc ttcatcaaca tggtcggtcc ccgcggtggc agtgccaaca aatttaatgg
    49501 gttttcctgc cagcaaagat tcattcactt tcgtgaccga acgcaccata agcgcatccg
    49561 cgtcggccag ttgagcaacg ggaattggac gcccgggaac cgcggtcacc tcacccaaac
    49621 ggctaaataa gtcgcgggca taaggcatat tttcatcaac aaggattttc acgtttgtgt
    49681 tacctgtatg agacgagagt taaccagaca agtgtgccat aatctcgcgg ccaggcatac
    49741 ttgcgaagat ttcaggtata aggatacgta atgatacaac ctatttccgg ccctcctcct
    49801 gggcaaccac caggtcaggg agataacctg ccgtctggcg cgggcaatca gcctttatcc
    49861 agtcagcaac gtacttcgct ggaaagctta atgacgaaag tgacctcact gacgcaacaa
    49921 caaagagcag aactgtgggc gggtatcagg cacgatattg gtctgtcggg agattcaccg
    49981 ctgctttcgc gtcacttccc tgccgctgag cataatctgg cgcaacgtct gttggccgcg
    50041 caaaaaagcc attctgcccg ccagctttta gcgcaattag gggagtattt acgtctgggg
    50101 aataatcgtc aggcggtcac ggattatatc cgtcataact ttggtcagac gccgctgaat
    50161 cagctctcac cggagcaatt aaaaaccatt ctcaccctgt tgcaggaagg gaagatggtt
    50221 attccgcaac cacagcagcg cgaggcgacc gaccgtcctt tattaccggc ggagcacaat
    50281 gcgctcaaac agctggtgac caaacttgcg gcagcaacgg gggaacccag caaacagatc
    50341 tggcaatcga tgctggaact ttccggggtg aaagatggcg agttaattcc agcgaaactg
    50401 tttaaccatc tggtgacctg gctacaggcg cgtcagacgc taagccagca aaatacgcca
    50461 acactggaat cactacagat ggcgctaaaa caacctttag atgccagtga actggcggcg
    50521 ttatcggcat atatccagca aaaatatggc ctttctgcgc aatcatcgct ttcttctgcc
    50581 caggccgagg atattcttaa ccagctttat caacggcggg ttaaagggat tgagccgcgt
    50641 gatatgcaac cgctgcttaa tccttttcca ccgatgatgg acacgttgca aaatatggca
    50701 acgcgtcccg cgctgtggat actgttagtc gcgattatcc tgattctggt ctggctggtt
    50761 cgttaacccc gacgaaacga cagtatcgtg acaataattc ccagcaccgc agagatcgcc
    50821 ccggcaagaa ataccgaaga gtaaccaaac gtggtcgcca gcattcccgc aagcggcccg
    50881 gagacgccga gggcgatatc ctgaaacgcg gcgtaaccgc ccagtgcggt gccgcgaacg
    50941 tgtgagggga cgcgtttaac cacctccacg cccagcgcag gaaagataag cgaacatccg
    51001 gctccagtta atgccgcgcc cgctaatgcg acccacgccc ctggggcttg ccagagcagc
    51061 aacaagccca ccgtttctac aagcagagag acgatcgcca ctttcacgcc gccaaaacgg
    51121 tccggcatcc agccaaacat gacgcgcatc acgacaaatg cgccgccaaa cgcggtaaga
    51181 gtaaagcccg ccatcgccca tcctttgctg gcaaagtaga gcgaaacgaa agtcccgata
    51241 accgcaaaac caacgccttg tagtgccaga cctaaccctg gtttccagat aagcccgaca
    51301 acgctccaca gcgatggacg ttcccccgcc agggccggta ctttgcgcac tgtgccgtta
    51361 caggcccacg ccagtaaggg taatgccatt gtggtgagcg ccagtgcggc aaaaccgtaa
    51421 tggctatgaa tcaacaggcc aagcggcgca ccaacagcga gggcaccgta aatagccatt
    51481 ccgttccatg acatcacttt gccggaatgt tttggcccaa cgatgcctaa tccccaggtc
    51541 agagcgcccg tcagtaactg gctttcacca aagccaagaa tcaaacgccc gacgaccaac
    51601 agagcaaatt tgaacggtgc ggggacgggc aaaatcgccg ccagcagtaa cgcgcccccc
    51661 gccagaccac aagctaacat cccctgaagc gccgaacgtt ttgcgccata ttgatcggcc
    51721 agtcgcccgg cgtaaccacg cgtcagcacc gtagccagaa actgaatccc gacggcaatg
    51781 ccgaccatag tgttgccata gcccagttca tgatgaacaa acagcgggat aaccggcaac
    51841 ggtaaaccta cggtcatgta ggtgagaaaa accgcaaaag cgatgcgaaa gagcgaaaaa
    51901 ttggcagaag atcgtgtttc ggtttggctt acagcagtca tgcattactc cagaatgcag
    51961 cgcaaggcga ggagtatccc cgtctcatct ctctggtttc agggttacgg tgcgttggca
    52021 ggatttaacg cgtacgtctt ttctgcagga aatcgacaaa gcgggaagtt tgcctggaac
    52081 tggcggcgat tgtcaatgat gtgaaaaagg gaaccatcgg gttccctttt gcgttagtgc
    52141 cggaggccgc attggcgcgt aacgtcggat gcggcgctgg cgcgtcttat ccgacctact
    52201 ccgaattaat ctttcagctt gcgcattacc agcgtggcgt tagtgccgcc gaagccgaag
    52261 ctgttagaca taactgtggt cagttcgcga tcggtcgttt cggtcacgat gttcagaccc
    52321 gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaaacc gtgttccagc
    52381 atcagcagag agtagatagc ttcctgtacg ccagcagcgc ccagtgagtg accggtcatt
    52441 gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagt
    52501 tctttcacgt cgccaaccgg agtcgaagta ccatgagagt tcaggtagtc gattggggta
    52561 tcaacaccgt gcatcgccat cttcatgcag cgcactgcac cttcgccaga cggagcaacc
    52621 atgtctgcgc cgtcagaggt tgcgccgtag ccaacgattt cagcatagat gtgagcacca
    52681 cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccaccgcc agcgataacg
    52741 aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta
    52801 gtagacagcg cacccatcgc gtcgaattcg caagccattt cccagcacag ctcttcgccg
    52861 ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata
    52921 cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccgtg aattttaaac
    52981 ggcgtggcga ggcaggcaga aacgccggat gccatcgctt tggtgaccac atacgggcca
    53041 accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc caaaaacctg gaaacgcggg
    53101 gaaccgccgc cggaacctgc aatcaggcca acgcgcgggt tattctggta agcttccggg
    53161 gagaggccag catctgcaat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg
    53221 ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt tacgttgccc
    53281 cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat ccctgaacgt
    53341 ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc
    53401 aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt
    53461 tcgcatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca attaatagag
    53521 aaatttgcgc agcctggcac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac
    53581 ttacgtgaaa cactacgcca tacaacctgc caaccttgaa tttaatgctg agggtacacc
    53641 tgtttcccga gattttgacg atgtctattt ttccaacgat aacggactgg aagagacgcg
    53701 ttatgttttt cttgggggta accaattaga ggcacgtttt cctgagcatc cacatcctct
    53761 gtttgtggta gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc
    53821 atttgatcag tttcgcgaag cgcatccaca ggcgcaatta caacgcttac atttcattag
    53881 ttttgagaaa tttcccctcg cccgtgcgga tttagcctta gcgcatcaac actggccgga
    53941 actggctccg tgggcggaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca
    54001 tcgtttattg ctcgatgaag gccacgtgac gctggattta tggtttggcg atataaatga
    54061 actgaccagc caactggatg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg
    54121 ctttgcgcca gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaagg ccatggcaag
    54181 gttagcgcgt ccgggcggca cgctggcgac ctttacgtct gccggttttg tccgccgcgg
    54241 tttgcaggaa gccggattta gcatgcaaaa gcgcaaaggg tttgggcgca aacgggaaat
    54301 gctttgtggg gtgatggaac agacattacc cctcccctgc tccacgccgt ggtttaaccg
    54361 tacgggcagc aacaaacagg aagcggcaat tattggaggc ggtattgcca gcgcgttgtt
    54421 gtcactggcg ctattgcgtc gcggctggca agtaacgctt tattgcgcgg atgaggctcc
    54481 cgcactgggt gcttccggca atcgccaggg ggcgctgtat ccgcttttaa gtaaacacga
    54541 tgaagcgcta aaccgttttt tctctaatgc gtttactttt gctcgtcggt tttacgacca
    54601 attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa
    54661 aagccagcat aaaatcgcac agatgttgtc gatggattta cccgcagaac tggctgtagc
    54721 cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggtattac
    54781 ttatccgcaa ggtggctggc tgtgtccggc agaactaacc cgtaatgtgc tggaactggc
    54841 gcaacagcag ggtttgcaga ttcattatca atatcagtta caggatttat cccgtaagga
    54901 tgattgttgg ttgttgaatt ttgcaggtga tcagcaagcg acgcatagtg tggtggtact
    54961 ggcgaacggg catcaaatca gccgtttcag ccaaacgtca tctctgccag tatattcggt
    55021 ggccggtcaa gtcagccata ttccgacaac gccggagctg gcgaaactga agcaagtgct
    55081 gtgctatgac ggttatctca cgccgcaaaa tccggcgaat cagcaccatt gtattggtgc
    55141 cagttatcac cgtggtagcg aagagacggc atacagtgag gaagatcaac tgcagaatcg
    55201 ccagcggttg attgattgtt tcccacacgc gcaatgggca aaagaggttg atgtcagtgg
    55261 taaagaggcg cgctgcggtg tgcgttgtgc tactcgcgat catctgccaa tggtaggcaa
    55321 cgtgcctgat tatgacgcta cactcgtgga atatgcgtcg ctggcggaga agaaagatga
    55381 agcggtaagc gcgccggttt atgacgatct ctttatgttt gctgctttag gttcgcgcgg
    55441 tttgtgttct gccccgctgt gtgccgagat tctggcggca cagatgagtg aagaaccgat
    55501 tccgatggat gccagcacgc tggcggcgtt aaacccgaat cggttatggg tgcggaagtt
    55561 gttgaagggt aaagcggtta aggcggggta atctgctctg gcattgtttg tcggatgcgg
    55621 cgtaaacgcc ttatccgacc tacgcgttac ctgtaggcct gataagacgc gataagcgtc
    55681 gcatcaggca tggtgctcca gacgtcacaa cttactgctg tgaggcctgc tgaaacaggt
    55741 gttcccacat atcggttacc agcgcctggt cacgcggtga caattcaccc gcgccaatgg
    55801 ctttttccag actctggcta acggttgtat gtaccgcctg agcggagtgg tcatcaccac
    55861 tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg gcaaacaact
    55921 catcatcact ggcatggtca accataccgt cgattaatgc cagaatgcgt gattcaaact
    55981 ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gcttcagttg agcgcttccg
    56041 gccacgggaa ttgttccgcc gtaagttccg gcgtgtgata ataattttgt agtgctttaa
    56101 tgaagcgtgc cggacgttcg ggaatgccgt ttgccagata atccatcacc tgcgcatgta
    56161 cccgccgttg aaacaccacg cggtccggtt cgaaatcccc ttccagattg tcgcagctaa
    56221 cattaaacgg atatcccgcc gccacgcaga acaaccagtc cagcgcctgc ggcttcactt
    56281 caacatcttc aaactgactt tgcgtctggg catcgcgtcc gtccgggcaa taccagtaac
    56341 cgaaatcaac cagctcacgg cgcgctttcc cggcaataca ccagtgcgaa atctcgtgga
    56401 tggcgctggc ataaaagcca tgagcgaaga cgattctgtt atacggtact tccgcatcag
    56461 caggaagata gatcggttcg tcgtctcctt taatcagacg ggtattaaat tcatcggcaa
    56521 agcagctatt aaaaatctca atcaactgct catagtggtg tgtactgttc attagctcat
    56581 ccccaaccag tggaggatct cctgtccatg gctatcgtaa agtaatttgg cactcatcac
    56641 cgccgagacg ataacgatca tcgggcggat cagcttttgt cctttgctca acaccagtct
    56701 tgaacccatg cgcgcgccga ggaactgccc caccagcatc acaaagcccg tcgcccaaat
    56761 cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg cgttaagtaa
    56821 tttggcgtga gcagtggctt tggcgaggtt gaatccgcac agcgtaacga aggccagcgc
    56881 gtaaaacgat ccggcagccg ggccaaagaa tccatcgtaa aaaccgacgc agcccccccc
    56941 caccagcgca aatggcagtc catacatccg acgctggcga tcttcttcac ccagctttgg
    57001 catcagcaag aaataaagac cgatacaaat caccagaatg ggcaaaatct gccgtaagac
    57061 atcagcctga acatactgca ccagcaatgc gccactcatt gagccgacaa aagtcatggc
    57121 gatattgagt ttttgatcgc ttaaactaac cactttacgg cgaataaagt agatagtggc
    57181 ggaaatagag ccaccgcagg cttgcagttt attggttgcc agcgcgttag cgggagacat
    57241 ccccgccgcc atcaatgccg gaatggtgag taacccacca ccaccggcaa tcgaatcgat
    57301 aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcgggg aaaccataaa
    57361 caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg gcaggaaggc
    57421 ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg tggttcaaac
    57481 cagctttgca gttctgcccc gcaaccatcg cctggtggcg gtaaaggttg atcttcacac
    57541 tccagactat cggcaggaca acgtaatcgt acatgcatat gcgcgcgatg ctggaaccag
    57601 ggtcgcactt tacgtaacca gtcgcgatcg gtgcccgcat caaggcaaag ttgttgttta
    57661 atcgccggat taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag tttgatcaag
    57721 ctgaaaattt ctggcttcca tagcgcgggg acaacgtgtt taccgtcgcg ggatactaag
    57781 tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc gtgtttttgg cagttgcagg
    57841 aagatatcga catccagccc ggtctggtgg ctggcgtgac cgccgttgaa acgaccacca
    57901 gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct cacctggcga
    57961 ctcagacgct ggataaacat caccagatcc gggtgcccga aatagcgacg ctgatcggta
    58021 cgcatgacct gataatgctc ggactgtatc ggcagcgtgt cagcgccgac gatacaacca
    58081 ttggaaaagc tgcctatcga ttgtgcgcta cccggcacag gttgggttat tttttgccac
    58141 ggcgtcgctg ccaggctggc gctactggca agcagggcca gcagcgcaat cgcggttttg
    58201 ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc ccgttgccgt
    58261 aacaggtgat ccatcaaaac gatcgccagc atcgcttctg cgatcggcac cgcacggatc
    58321 ccgacacaag gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc aaagcggtta
    58381 atagtgcgcc ccggcacagt aatgcttgag gtcggtttca gcgccatatg ggcaatgatt
    58441 tgctgcccgc tgctgatacc accaagaatg ccgcctgcat ggttgctctg gaaaccgtct
    58501 ttggtgattt cgtcacggtt ctggctgcca cgcagcgcta ccacgtcaaa accatcacca
    58561 atttccacgc ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc ggcatccagg
    58621 cggtcaaaga ccggctcgcc aagtccggcg gggacgccac tggcaacaac ggtgactttc
    58681 gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa cgcgtcgatt
    58741 ttgtccgggt ccgggcagaa aaacggattt tgctcgacct gcgaccagtc tttgatttcc
    58801 agcggaatgt cacccatctg ggtcaggcag ccgcggattt caataccaaa tttctcggcg
    58861 agatattttt tggcaatcgc ccctgccgcc acgcgcatgg cggtttcacg ggcggaagag
    58921 cgcccgccgc cgcgataatc gcgcaggccg tatttttgtt cgtaagtgta atcggcatga
    58981 cctggacgga aaacgtcctt aatcgcacta taatcctgag aacgctggtc ggtattttcg
    59041 atcaacaaac cgatgctggt gccggtagta acaccttcaa aaacaccgga gagaattttg
    59101 acctgatccg gctcacgacg ctgggtggta tagcgcgatg tcccagggcg acgacggtcg
    59161 aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc atcgacgatg
    59221 cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag ttgtccaatt
    59281 gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt aatctttata
    59341 aatcgcgaaa tgttcacggg ccgccagcag ctgctctttg gtgagcataa acacaccatc
    59401 gccgccgtta tcaaactcca gccaggtgaa cggaacatcc ggatattgtt ccataagatg
    59461 taccatgctg ttgccgactt cacaaatcag cacgccatca tcagcgaggt aatctgccgc
    59521 gtttccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca ggcccagttc
    59581 cggctcgtgg cggtattcgt ttggcaggtc ggacatatct tctgcatcga cgtacggcgg
    59641 gttggtgacg atcaggtcgt actgcacttt cggcaagtca cggaacaagt cggaacgaat
    59701 cggaatgacg ttgtggatca aaccgtgttc ttcgatgttc tgttcagcaa ccgtcagcgc
    59761 gtctggagag atgtccaccg cgtcgacttc cgcttccggg aaggcataag cacaggcaat
    59821 ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgct tgctgatgag
    59881 tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca gcacgcgttc
    59941 atcgacgtaa aattcatggc cgcagaacca ggctttgttg gtcaggtaag ccaccggaat
    60001 gcgctcgttg acgcggcgga tcacgcgctc aacaatacgg tgtttttcgc tggaggtcag
    60061 acgcgcggtg cgcatgtctt ccggaatatc cagcggcagg tagagcgaag gcaacaccag
    60121 ttgcacggct tcatcccacg ggttatcggt gccgtggccg taccagatat tcgccgcgct
    60181 gaagcggctc accgaccagc gcaacatgtc ctgaatggtt tgcagctcat ttactgcttc
    60241 atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat ttcggcggct
    60301 agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag gaaaaatacg
    60361 tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag aaatagagac
    60421 agttaaatga aaaagaaaac aacacttagc gaggaggacc aggcgctgtt tcgccagttg
    60481 atggcgggga ctcgcaagat taagcaggac acgattgtcc atcgaccaca acgcaagaaa
    60541 atcagcgaag taccggtcaa gcggttgatt caggagcagg ccgatgccag ccattatttc
    60601 tctgacgagt ttcaaccatt attaaatact gaaggtccgg tgaaatatgt tcgcccggat
    60661 gtcagccatt ttgaagcgaa gaaactgcgg cgcggtgatt attccccgga gttgtttctt
    60721 gatttgcacg gtctgacgca gctacaagcc aaacaggaac tgggggcgtt gattgccgcc
    60781 tgccgccgtg aacatgtgtt ttgcgcctgt gtgatgcatg gtcatggtaa gcatattttg
    60841 aagcagcaaa cgccactgtg gctggcgcaa catccccatg taatggcatt tcaccaggca
    60901 ccaaaagagt atggtggtga tgcggcattg ctggtgttga tagaagtaga tgagtggctg
    60961 ccgccgaaat tgccctgaat atttaaaggt ggacgagtct gtccatttag ccttgaatac
    61021 tggcgtctca accctggcga taatgaacgt aataatcggg cattaattga aaaacgtaag
    61081 cagctttccg acagccttct gtgggctaat ggcattgccc cttcagaggg gcttgaggat
    61141 gacgacctgc tgcaagcgct tatcgacgaa ttactcaccc tcgtcgacgg ttatctggca
    61201 cctttcgctc ccgtgatggc gaatattcta aaaaactgga tgaccgcatg acataacgga
    61261 caacggggcc agatggcccc gttgtgtggt gtatacgcta cagctcgttg agcgtgtact
    61321 ggaagcggca ggtggaatca atcggctggc ggctgctggt atcgacacgc accaccatgt
    61381 cgtgcagttg cttgtcagtg atgggatgcc tgtttggcgc caccgtaaca ttagcattat
    61441 cacagcctga tgatgaaaac gtgatcattt gcgcaaccca gcgacagtaa gcgccctctt
    61501 cattcatttg cgagccatca catagtttgc cgtctttcat cagataggtt gaaagcgttt
    61561 tttcaatcac accccccgtc gattgcaaag acagtgtttt cgttttattg atatagtttt
    61621 catcccacga aacatcatac ggcgtaactg acacggttgg gcacacggac acggttgcat
    61681 ttggtccata atagatatct ttatcaacag aaccgaccac aacctggctg gtggctctga
    61741 tactcactaa ccacgggcag cctcccacat caccatcgac ggtaatatcc agaggcagct
    61801 tgagacattc ccagcggctg tctttggctt ccagtgcctc catgcccgga caaaatgaat
    61861 tatttacaat tgtcccatct tttaccgtaa tcgtcggcgc aggagaactg cccgttcctg
    61921 tccatgtgcg attgggtaag ttgccaagcg tcataacata attactttga taataggtac
    61981 cgtagtaaac actcgaaacc tcagcaacat caagatacag ttcgacctga ctattccagc
    62041 caaacgtagc atagtagata tacacattac gaaatgtctg ccccatcccg gcccgactac
    62101 cgagcataaa aaccatcagg atcaaaagac gaaacagcaa gttagttttt ttcatcttat
    62161 atgtactcaa acgttgcggt taaggaggcg gtaaactcac ccatcgtcac ttcccgcccg
    62221 cttttcgcct ggagccaggc attaaaattc agattgttat tgccgttact cagcacaaag
    62281 gtcgcgccgc tggtattgtt aatcggcacc ggtgttccat cgtttttttc catgccaatt
    62341 ccgacacctt gcgccgccga agatacatcc agcgccagaa aaccgggctg ttcactgtct
    62401 tctgtaccgg tcagcgtcac ccgaacctca tactgagctg gccctttgca atctttgagc
    62461 tgaaacacca ctggcacccg cgccgactgc ccggtcacca tcaggtcttt acggctgatg
    62521 gtgggaaaat gtacctccgc cagcgtttcg ccctggacca ccagcgtgca ggatttactg
    62581 agtaaattgc cgtagaaatg caggttgcca gccgcctgaa catggggcgt cagcagcacc
    62641 gtcgccagta cagccgcgca gagagatatc cgttttaggt tcattggtaa tccacctgta
    62701 atgttgcgta ggcagaaaag gttccgtcgc tgagcgccgc atcattggct ttcaccggca
    62761 cagcttgcag cgttggcggc gttgacgggt caattttcaa cggtgttccc agcttaaacg
    62821 gctggccgtt ttgctgtagc tcaatcccca ggccgctaac gtccgtttca atggcggaat
    62881 catcataagg ggtttggctg cccgtccagg tcaggctcat ctcccaggcg tcgtcgcgca
    62941 catcggggtc acatgtgatg gtgtaaggca catcctgacg gaaattatcg ccgttaatgt
    63001 tgtcgataat gacgttgcga aactcaactt cgatcgtctt accatcgctg atagtacagg
    63061 cgggtggcga aaccagcgtg ccgtgaaaag taacgtcgtt aatcgcggcc tgcgccaggc
    63121 cagcaaaacc gtaaattaag caccatgcga gtcgtttcat tggtagtcca ccaccatggt
    63181 catggtcgca ttaaactccg atgccatcag ttccacaccg ctctgcttca caggaacggc
    63241 ttcgaagtct ggctgagtat tgatgttgaa cggcagccag ctactgttac ccacggccac
    63301 cagcgaatgg tcagcggcat tctgaatacg aatgccaaaa ccgctgattc cagtactcag
    63361 caccgtttcg ccgttgatgg tcgtggtggt ggcctgaagc tgcatttgca ggtcgtcgct
    63421 gacactgttt gagcagttca gggtgtagcc cactgcctga cggtagttgg caccatcaat
    63481 gcttttaata atcacattgc cgaactccac cgcgcttccc tttaccgaac agggcggcgg
    63541 tccatccacc agcacgcgca acgtcagatt gacgattttg ccgcccgcca ttgccgggaa
    63601 aacggtcacg ccacaggcca gcaatagcgc ggcagatccg gtcagaaaga acgctttcat
    63661 gttgcttctc ccgttatcaa cctttgctgc tttgatcttc atccaccttg caggtgttcc
    63721 cttcacaacg gaagatcagc ggcatgcgcg cgccgtagtc gttgatatag gtcagcaccg
    63781 gggcgctcgc catttttgcc tttaacggtt gtgacgtttt cggtggcatc accagcggtt
    63841 taaacccggc ggcagtcagc cctttcttct gtgtgctggc atcgctgatg atgacgtggt
    63901 acggcgtcgg gttattgacg gtatagtcct ggccgctgcg cgtgagtgtg accttaaact
    63961 gccacggttt tctcatgtca atcttctcca gcgcctttgg tcgccagaac agcttgatgc
    64021 gcgtttgcag cgcaatttgc agagtgttgg ctttcccgga ctttggcggg atctcacgca
    64081 cgttgtagta gaacaggctt tctcggtcgg acggcagcgt gtgaatgtcc ggcagcgcct
    64141 gaatcttcac ctggccgttc atcatagcgt caatacgctg caccggcggc agtactgcca
    64201 gcggcgagat gattttgttg cccttctcgt cttccagcca gctttgcgcc aggtaaggca
    64261 gcttttcgtt gttgttacgc aacgtcacgc tgatcgattt atcgctttcg ttaaacacca
    64321 gacgggtgcg atccggggtg accgatgcat gcgccgtcag gctggcggtg gtaagcattg
    64381 ccatcagcgc tgtgctgacg agcattggac tggataattt ataagacatt gatcgttcct
    64441 taatcagatt attgagttgc tgaaattgac gtcggcgctt ccgttggtgt gacttgccgc
    64501 gtctggtcct gaataacagg cttaatttct ggcgctgaag aatcaggggc tgccgttccc
    64561 ttttgttcac acggcaacaa caggccgctg aacagatcgg caggtagcgg cttcggcaat
    64621 acgatctcgc attgtgcgct gccttcccag aacaccatca tatgctcgcc cgcattgacc
    64681 cccgccagat agacattgcc ttcatcatcc acaatgccaa cctgctgctg ctcgtcgttt
    64741 ttcacttccg cgccaaacgg cgggtagcta ccgtcacgca accgcagtac cgccatcgct
    64801 ttttgcccgc tgatcacttt gaatttacga tagccgattg cgccttccgt cagagtcgcc
    64861 tgcaccaccg actgggtggc ttccgcatct tccggcaagt tgtttaaatc gatatacgcc
    64921 tgattgcgat agtagttgtt aatatcggcg accaccgcct tgccaaacat attggtgtac
    64981 accggcgcgc cgttgctttc gaccggcacg ttggcaatcc cgtcggcgtc aatcagcagg
    65041 cgcgtgccgc ccatgttctg ggtacggtgc agcgccccac catgcgcggt cagcgttgcc
    65101 ccgccctgta aggcgatccc cgccgaacgg tattcccctt catggtagtt ggcgctgaga
    65161 tcaaccttcg ccagcgaacc gtcgtggctc agataaccat ccacgctgcc gtgctgctcg
    65221 ctggtgccaa cgttaacctg gtagtgcgtt gcgtcatcga cgcgcttaaa gtaaccgacc
    65281 tggctgctgt ccgacccgct gccgtaggaa ccgttgtagg tcacggtgct gctgtcgctc
    65341 cacggaatgc tcatactgag gtacatcccc ttatccgcat tatcgtcata ttcgtagcga
    65401 tagccggtca ccgagatgct catgttgcga atgctcccca tattaaaata gtgggaaaac
    65461 atcaagttat agtttgtctg ttccgggcga tcccagtaag tacgatggga gtagttgaga
    65521 tagatcgaaa cacccgcagc ggcaaagttc tggttatagg tgatcgtata catctctttg
    65581 tcgttaccgg tccgcgccat gtctgactgg ttcgcgtcca gatactcgct catggtcatg
    65641 aagttctttt cagaaaaacg gtagcctgca aaggtgacgc ggctgttgag ttcgtcaaag
    65701 tctttggcat agctcacgcg aaaggagttg ccgtccagtt ttcctttgcc gtatgcgctg
    65761 tcatgatcca ggttgacgtg cgagtgagtc acatcgaacg ccagcgcgcc aaactgtgcg
    65821 aggtcgcgcc ccacccccat cgccgccgac tgatagtgtt tatctgccag cgcaccaccg
    65881 tagagcgacc agccatctgc caccccccat gacgcttctc cgccggagaa aaagccgcct
    65941 tcggtcttgt ggttccagtc ttccggacgc cccatcatca ctttgtaacg cacctgcccc
    66001 tggcgcgtga ggaatggcat agatgcggtg gtaacgtcat attcctgcac ctgaccattc
    66061 tgttcttcaa cgcggacgtg cagtgtgccg gagacggagt cgccgatatc ctgaatgcgg
    66121 aatggcccgg cgggaacctg ggtttcgtaa agtacccggc ccatctggct aatggtgact
    66181 tttgcactgc tgtgcgccac gccggaaaca tccggtgcat agccgcgcag gttgggcggc
    66241 agcatctggt catcggtgct gacgctgctg ccgatatagt taaagccgtc gaaaatatcg
    66301 gaattgagat aatctttccc cagcgacagc ttcgctttta aagagggcaa ggctcgccag
    66361 gcataataac gactccagtc ccagtgttta ctcgttgtgc tgttactgct gtcatcgtcg
    66421 tcatcgttgc tgcgggtgtg ctgataatca ctttgccagt ctgcacggaa acgccatgcc
    66481 cccaggttcg ccccaacggt accgttgccg ctgatatcat gtgagtcctc gcccccctgc
    66541 tcctggtggc gagtttgcgc attgaggctg tagtcggcaa tcaggccagg aataccgtcg
    66601 tcccaacgtg agggaggatc ccagtcgctg ctggtatatt cgaggtaagc ctgtggcact
    66661 gtcagcagca acgccgactg gcttaaatca ttctcgactt ccatcccgtc taattgacct
    66721 ggtttaaggc attcatcgtt gtgcgtccac tgcaggcttt ttgctatccc ttctttcagc
    66781 cccagcgccg ccactaactc aggtgtcagg caggcatagt tttttgttgg atcgttttca
    66841 gagacgtacc agttaatgtc gtattcatca gaaaggggtt gtttatttat aaatacacgt
    66901 aaattatatt tacccgcgtc gacataccct ttttttgaaa acttaccgag atcaattttc
    66961 gtatcgccct ttaactcaag gaaacggggg gcaaattgaa ttgaatcatc agcccatgca
    67021 ttgactgacc caccagagat tgccagcgca atatagcagg cgattccccg cagccgaaaa
    67081 tttgagtgat taggcataca catatccgtt tgtgaaacag tcgtaaatct gttgcgtgtt
    67141 gaataacccc acagcagaac gctgtggggt tgtcacatca gagataagta atctggaatg
    67201 tggtcagggt ttcgaactgg ccgagatccg gcgcatcggt ttcacccacc agccaggctt
    67261 taaagttcag cgtttgcttg tctttaccct tgctggttga agtatccatc acgattggct
    67321 gaacaatctt ttcgcccact ttataacctt tgccatgggt atcgccgatt accaggctca
    67381 cgtggttcat ggcaacaccg ctgtcaacat tgaataacgc atagttatcc gtgttggctg
    67441 gccccgcgta gttagtaccg gtgaacgtgg tctccatgtt tgtttcagtg gtaaacacgc
    67501 aatcctgtaa acggatctgg aaatctttcg gtgtggtagt gccaccgccg gtcagtttag
    67561 ccgcaccgat gtcacccata tcgacttcca gtttgtgatc gtctggaacg atagagcacg
    67621 gggagtcttc aatggtgccg tagaagcgcg cggtaccgtt gttgcctgct gcaaaagcag
    67681 aactggaaac aactgcgccc atgaccatcg ccgcagcaat ggctgttttg gcaaacttac
    67741 tcattatttg tccttaaatc attaagagtt ataatataag cacctgacat aaaattaata
    67801 tatattatgc c
//
LOCUS       DAZGJT010000010        67174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZGJT010000010 DAZGJT010000000
VERSION     DAZGJT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.010"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            59..637
                     /locus_tag="ACHKFK_000717"
     CDS             59..637
                     /locus_tag="ACHKFK_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9051132.1"
                     /translation="MKDYSHVKKTLLSMFATALLSGVAFNALADDANQGSGKITFKGE
                     VIDALCSIAPGDEDQTINLGEVADTVLKSGQKSLPVDVTIHLQDCILSDGTNTVDKVK
                     ITFSSASVDATDSNLLKNTLEGNIGGATDVGVRLVKSDNTNVTLGTPITINFPTTNSY
                     QELNFKARMESLGRTATPGNVQAQANYVLDYK"
     gene            724..3246
                     /locus_tag="ACHKFK_000718"
     CDS             724..3246
                     /locus_tag="ACHKFK_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO9051133.1"
                     /translation="MNNKNTFSRDKLSHAIKNALSGVVCSLLFVLPVHAVEFNVDMID
                     AEDRENIDISRFEKKGYIPPGRYLVRVQINKNMLPQTLILEWVKADNESGSLLCLTKE
                     NLTNFGLNTEFIESLQNIAGSECLDLSQRQELTTRLDKATMILSLSVPQAWLKYQATN
                     WTPPEFWDTGIAGFILDYNVYASQYAPHHGDSTQNVSSYGTLGFNLGAWRLRSDYQYN
                     QNFADGRSVNRDSEFARTYLFRPIPSWSSKFTMGQYDLSSNLYDTFHFTGASLESDES
                     MLPPDLQGYAPQITGIAQTNAKVTVAQNGRVLYQTTVAPGPFTISDLGQSFQGQLDVT
                     VEEEDGRTSTFQVGSASIPYLTRKGQVRYKTSLGKPTSVGHNDINNPFFWTAEASWGW
                     LNNVSLYGGGMFTADDYQAITTGIGFNLNQFGSLSFDVTGADASLQQQNSGNLRGYSY
                     RFNYAKHFESTGSQITFAGYRFSDKDYVSMSEYLSSRNGDESIDNEKESYVISLNQYF
                     ETLELNSYLNVTRNTYWDSASNTNYSVSVSKNFDIGDFKGISASLAVSRIRWDDDEEN
                     QYYFSFSLPLQQNRNISYSMQRTGSSNTSQMISWYDSSDRNNIWNISASATDDNIRDG
                     EPTLRGSYQHYSPWGRLNINGSVQPNQYNSVTAGWYGSLTATRHGVALHDYSYGDNAR
                     MMVDTDGISGIEINSNRTVTNGLGIAVIPSLSNYTTSMLRVNNNDLPEGVDVENSVIR
                     TTLTQGAIGYAKLNATTGYQIVGVIRQENGRFPPLGVNVTDKATGKDVGLVAEDGFVY
                     LSGIQENSTLHLTWGDNTCEVTPPNQSNISESAIILPCKTVK"
     gene            3262..4002
                     /locus_tag="ACHKFK_000719"
     CDS             3262..4002
                     /locus_tag="ACHKFK_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9051134.1"
                     /translation="MKSPLITTGMICLLGICNFAQATVSPDRTRIIFNASNKSATVRL
                     TNQSKIDPYLAQSWIEDASGKKTRDYISTLPPMERIEPDEQIQIRLMALASLNDLPQD
                     RETLFYYNVREIPPRAKEQNVMQIAMQSRLKLFWRPKAIELKEGEMIPLQKVTITRTA
                     AGLTLNNPTPYHITVGYIGTNGKTLMPGADSIMVAPFTSATQHLSSLPSTFQLGFVAD
                     YGGLEMFKVECNSIQSLCQSSPAKKGKI"
     gene            3999..5048
                     /locus_tag="ACHKFK_000720"
     CDS             3999..5048
                     /locus_tag="ACHKFK_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO9051135.1"
                     /translation="MITLFRLLAILCLFFNVSAFAVDCYQDGYRGTTLINGDLPAFKI
                     PENAQPGQKIWESGDINITVYCDNAPGWTTDNPSENIYAWIKLPQINSADMLNNPYLT
                     FGVTYNGVDYEGINERIDTHACLDKYEQYYNGYYYDPVCNGSTLQKNVTFNAHFRVYV
                     KFKSRPAGDQTVNFGIVNVLQFDGEGGANMSSNAKNLRYVITGLDNISFLDCSVDVRI
                     FPESQIVNFGQIAANSIATYRPKAAFSVSTIKDVAADCTEQFDVATSFYTTDTLHDDT
                     HLEMGNGLLMRITDQKTQEDIKFNQYKRFTTYIPGQTGAMVTRDYQAELSQKPGETLV
                     YGPFKKDLIVKINYN"
     gene            complement(5102..5755)
                     /gene="ribB"
                     /locus_tag="ACHKFK_000721"
     CDS             complement(5102..5755)
                     /gene="ribB"
                     /locus_tag="ACHKFK_000721"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO9051136.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITDDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(5859..6007)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            6129..6428
                     /gene="ubiK"
                     /locus_tag="ACHKFK_000722"
     CDS             6129..6428
                     /gene="ubiK"
                     /locus_tag="ACHKFK_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311957.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO9051137.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELEARNNPVAELQSPPAIPPVDKAE"
     gene            complement(6463..6663)
                     /gene="glgS"
                     /locus_tag="ACHKFK_000723"
     CDS             complement(6463..6663)
                     /gene="glgS"
                     /locus_tag="ACHKFK_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO9051138.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQTRELELEH"
     gene            6933..7562
                     /gene="yqiJ"
                     /locus_tag="ACHKFK_000724"
     CDS             6933..7562
                     /gene="yqiJ"
                     /locus_tag="ACHKFK_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9051139.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEIFALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACVTLWQSPLPNLF
                     VVPVSLLFTIIAVHYTGKVVAPWIPRDHSSAITEEEYVGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKTFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            7589..9250
                     /gene="yqiK"
                     /locus_tag="ACHKFK_000725"
     CDS             7589..9250
                     /gene="yqiK"
                     /locus_tag="ACHKFK_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO9051140.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLSGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNAKIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGATGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSSLSSTTPVEEKAE"
     gene            9490..9549
                     /locus_tag="ACHKFK_000726"
     CDS             9490..9549
                     /locus_tag="ACHKFK_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9051141.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(9669..11102)
                     /gene="hldE"
                     /locus_tag="ACHKFK_000727"
     CDS             complement(9669..11102)
                     /gene="hldE"
                     /locus_tag="ACHKFK_000727"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO9051142.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(11150..13990)
                     /gene="glnE"
                     /locus_tag="ACHKFK_000728"
     CDS             complement(11150..13990)
                     /gene="glnE"
                     /locus_tag="ACHKFK_000728"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO9051143.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEESLSAQAKSVLTFSDFLQD
                     SIIAHPEWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSDGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPFDELNRARLTWAMDFADWPQLTGVLTAHMANVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLMLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSENCFTAERELVRASWQKWLVEE"
     gene            complement(14013..15314)
                     /gene="ygiF"
                     /locus_tag="ACHKFK_000729"
     CDS             complement(14013..15314)
                     /gene="ygiF"
                     /locus_tag="ACHKFK_000729"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO9051144.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPTDLASRVQPLFSTDFYREKWLVEVDGSQIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAAFELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQSKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDLILLLAGYYDPVVAQ
                     DWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            15556..16176
                     /gene="ygiM"
                     /locus_tag="ACHKFK_000730"
     CDS             15556..16176
                     /gene="ygiM"
                     /locus_tag="ACHKFK_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO9051145.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            16240..17478
                     /gene="cca"
                     /locus_tag="ACHKFK_000731"
     CDS             16240..17478
                     /gene="cca"
                     /locus_tag="ACHKFK_000731"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO9051146.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLCFRIADETLALM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVANWKEQRCPKSE"
     gene            complement(17660..18481)
                     /gene="bacA"
                     /locus_tag="ACHKFK_000732"
     CDS             complement(17660..18481)
                     /gene="bacA"
                     /locus_tag="ACHKFK_000732"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9051147.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(18572..18940)
                     /gene="folB"
                     /locus_tag="ACHKFK_000733"
     CDS             complement(18572..18940)
                     /gene="folB"
                     /locus_tag="ACHKFK_000733"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO9051148.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            19045..19662
                     /gene="plsY"
                     /locus_tag="ACHKFK_000734"
     CDS             19045..19662
                     /gene="plsY"
                     /locus_tag="ACHKFK_000734"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO9051149.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(19675..20607)
                     /gene="ttdR"
                     /locus_tag="ACHKFK_000735"
                     /gene_synonym="dan"
     CDS             complement(19675..20607)
                     /gene="ttdR"
                     /locus_tag="ACHKFK_000735"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO9051150.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDAIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQIM"
     gene            20814..21725
                     /gene="ttdA"
                     /locus_tag="ACHKFK_000736"
     CDS             20814..21725
                     /gene="ttdA"
                     /locus_tag="ACHKFK_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO9051151.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIVPDGDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLRVHADLTFENLSHTRSAL"
     gene            21722..22327
                     /gene="ttdB"
                     /locus_tag="ACHKFK_000737"
     CDS             21722..22327
                     /gene="ttdB"
                     /locus_tag="ACHKFK_000737"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO9051152.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLIGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            22375..23838
                     /gene="ttdT"
                     /locus_tag="ACHKFK_000738"
     CDS             22375..23838
                     /gene="ttdT"
                     /locus_tag="ACHKFK_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO9051153.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPLPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASNATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPILKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIICWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTIVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(23881..24894)
                     /gene="tsaD"
                     /locus_tag="ACHKFK_000739"
     CDS             complement(23881..24894)
                     /gene="tsaD"
                     /locus_tag="ACHKFK_000739"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO9051154.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     DVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            25132..25347
                     /gene="rpsU"
                     /locus_tag="ACHKFK_000740"
     CDS             25132..25347
                     /gene="rpsU"
                     /locus_tag="ACHKFK_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO9051155.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            25458..27203
                     /gene="dnaG"
                     /locus_tag="ACHKFK_000741"
     CDS             25458..27203
                     /gene="dnaG"
                     /locus_tag="ACHKFK_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO9051156.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADRNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            27398..29239
                     /gene="rpoD"
                     /locus_tag="ACHKFK_000742"
     CDS             27398..29239
                     /gene="rpoD"
                     /locus_tag="ACHKFK_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO9051157.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDTADDDNSIDPELAR
                     EKFAELRAQYIVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(29317..29823)
                     /gene="mug"
                     /locus_tag="ACHKFK_000743"
     CDS             complement(29317..29823)
                     /gene="mug"
                     /locus_tag="ACHKFK_000743"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO9051158.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QSLVVRGR"
     gene            29948..30023
                     /locus_tag="ACHKFK_000744"
     tRNA            29948..30023
                     /locus_tag="ACHKFK_000744"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29981..29983,aa:Ile,seq:cat)
     gene            complement(30077..30841)
                     /gene="nfeF"
                     /locus_tag="ACHKFK_000745"
                     /gene_synonym="yqjH"
     CDS             complement(30077..30841)
                     /gene="nfeF"
                     /locus_tag="ACHKFK_000745"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO9051159.1"
                     /translation="MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAKPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            31129..31752
                     /gene="nfeR"
                     /locus_tag="ACHKFK_000746"
                     /gene_synonym="yqjI"
     CDS             31129..31752
                     /gene="nfeR"
                     /locus_tag="ACHKFK_000746"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO9051160.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKNQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(31859..33379)
                     /gene="aer"
                     /locus_tag="ACHKFK_000747"
     CDS             complement(31859..33379)
                     /gene="aer"
                     /locus_tag="ACHKFK_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO9051161.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGYT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAVMLWFVAAPVVTYILCALVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRVVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            33797..35176
                     /gene="ygjG"
                     /locus_tag="ACHKFK_000748"
     CDS             33797..35176
                     /gene="ygjG"
                     /locus_tag="ACHKFK_000748"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO9051162.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(35218..35550)
                     /gene="ygjH"
                     /locus_tag="ACHKFK_000749"
     CDS             complement(35218..35550)
                     /gene="ygjH"
                     /locus_tag="ACHKFK_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO9051163.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRIV"
     gene            35769..36752
                     /gene="ebgR"
                     /locus_tag="ACHKFK_000750"
     CDS             35769..36752
                     /gene="ebgR"
                     /locus_tag="ACHKFK_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO9051164.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTSALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDLPGKADIREVAFAEYGRLKHVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            36935..40027
                     /gene="ebgA"
                     /locus_tag="ACHKFK_000751"
     CDS             36935..40027
                     /gene="ebgA"
                     /locus_tag="ACHKFK_000751"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO9051165.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKQLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLATSPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSARF
                     AFTVEQPQQWSAESPYLYHLVMTLKDADGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWENVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKT
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFVVEQ
                     SDDEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            40024..40473
                     /gene="ebgC"
                     /locus_tag="ACHKFK_000752"
     CDS             40024..40473
                     /gene="ebgC"
                     /locus_tag="ACHKFK_000752"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO9051166.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            40536..41969
                     /gene="ygjI"
                     /locus_tag="ACHKFK_000753"
     CDS             40536..41969
                     /gene="ygjI"
                     /locus_tag="ACHKFK_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9051167.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            42103..43173
                     /gene="ygjJ"
                     /locus_tag="ACHKFK_000754"
     CDS             42103..43173
                     /gene="ygjJ"
                     /locus_tag="ACHKFK_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO9051168.1"
                     /translation="MKLITAPCRALLALPFCYAFSVTADEARPAEHDDSKTPAMTSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLTEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQLFLMQYFYW"
     gene            43190..45541
                     /gene="ygjK"
                     /locus_tag="ACHKFK_000755"
     CDS             43190..45541
                     /gene="ygjK"
                     /locus_tag="ACHKFK_000755"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO9051169.1"
                     /translation="MKIKTILTPVACALLMSFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLISKDVQVEMILRFATPRTSLLETKITSDKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVIFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKNWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTDNGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            45767..47785
                     /gene="fadH"
                     /locus_tag="ACHKFK_000756"
     CDS             45767..47785
                     /gene="fadH"
                     /locus_tag="ACHKFK_000756"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO9051170.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDANQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVGAVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIAGFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQILAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(47830..48246)
                     /gene="higA"
                     /locus_tag="ACHKFK_000757"
     CDS             complement(47830..48246)
                     /gene="higA"
                     /locus_tag="ACHKFK_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO9051171.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(48243..48557)
                     /gene="higB"
                     /locus_tag="ACHKFK_000758"
     CDS             complement(48243..48557)
                     /gene="higB"
                     /locus_tag="ACHKFK_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFO9051172.1"
                     /translation="MHLITQKALKDAAEKYPQHKAELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(48842..49978)
                     /gene="rlmG"
                     /locus_tag="ACHKFK_000759"
     CDS             complement(48842..49978)
                     /gene="rlmG"
                     /locus_tag="ACHKFK_000759"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO9051173.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTPDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            50063..50566
                     /gene="ygjP"
                     /locus_tag="ACHKFK_000760"
     CDS             50063..50566
                     /gene="ygjP"
                     /locus_tag="ACHKFK_000760"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO9051174.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            50643..51335
                     /gene="ygjQ"
                     /locus_tag="ACHKFK_000761"
     CDS             50643..51335
                     /gene="ygjQ"
                     /locus_tag="ACHKFK_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO9051175.1"
                     /translation="MLRVFAHLLRRVCFSRRTLKIGCLLLLIAGATLFIADRVMVNAS
                     QQLTWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRANKVFSENHITIISQEFHNQRA
                     IWLAKQYGIDAIGFNAPDLNMKHGLYTQLREKLARVSAVIDAKILHRQPKYLGPLVMI
                     GPFSEHGCPAKE"
     gene            51414..52400
                     /gene="ygjR"
                     /locus_tag="ACHKFK_000762"
     CDS             51414..52400
                     /gene="ygjR"
                     /locus_tag="ACHKFK_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9051176.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTAYLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPTFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVNHPGLAVSRITAKLLTEIRRQTGVIFPADNVKL"
     gene            52683..53648
                     /gene="alx"
                     /locus_tag="ACHKFK_000763"
     CDS             52683..53648
                     /gene="alx"
                     /locus_tag="ACHKFK_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO9051177.1"
                     /translation="MNTVGTPLLWGGFAVVVTIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRVG"
     gene            53972..55216
                     /gene="sstT"
                     /locus_tag="ACHKFK_000764"
     CDS             53972..55216
                     /gene="sstT"
                     /locus_tag="ACHKFK_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO9051178.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLILGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVIF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIHFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAATCQAEDDRLANSALRN"
     gene            complement(55220..55771)
                     /gene="ygjV"
                     /locus_tag="ACHKFK_000765"
     CDS             complement(55220..55771)
                     /gene="ygjV"
                     /locus_tag="ACHKFK_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO9051179.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQRRGIDPFKVEKTPSAVDERG"
     gene            complement(55854..57341)
                     /gene="uxaA"
                     /locus_tag="ACHKFK_000766"
     CDS             complement(55854..57341)
                     /gene="uxaA"
                     /locus_tag="ACHKFK_000766"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO9051180.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIAHLHQLYSVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            complement(57356..58768)
                     /gene="uxaC"
                     /locus_tag="ACHKFK_000767"
     CDS             complement(57356..58768)
                     /gene="uxaC"
                     /locus_tag="ACHKFK_000767"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO9051181.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            59250..60548
                     /gene="exuT"
                     /locus_tag="ACHKFK_000768"
     CDS             59250..60548
                     /gene="exuT"
                     /locus_tag="ACHKFK_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO9051182.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVDTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            60678..61454
                     /gene="exuR"
                     /locus_tag="ACHKFK_000769"
     CDS             60678..61454
                     /gene="exuR"
                     /locus_tag="ACHKFK_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO9051183.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDARTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            61694..61777
                     /locus_tag="ACHKFK_000770"
     CDS             61694..61777
                     /locus_tag="ACHKFK_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051184.1"
                     /translation="MLMYQTRRTYQDSNNIAVVHHLKPAWR"
     gene            61799..62461
                     /gene="yqjA"
                     /locus_tag="ACHKFK_000771"
     CDS             61799..62461
                     /gene="yqjA"
                     /locus_tag="ACHKFK_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO9051185.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            62465..62848
                     /gene="mzrA"
                     /locus_tag="ACHKFK_000772"
     CDS             62465..62848
                     /gene="mzrA"
                     /locus_tag="ACHKFK_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO9051186.1"
                     /translation="MQIPRMSLRQLAWSGAVLLLVSTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            62995..63363
                     /gene="yqjC"
                     /locus_tag="ACHKFK_000773"
     CDS             62995..63363
                     /gene="yqjC"
                     /locus_tag="ACHKFK_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO9051187.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHQKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLKARDY"
     gene            63401..63706
                     /gene="yqjD"
                     /locus_tag="ACHKFK_000774"
     CDS             63401..63706
                     /gene="yqjD"
                     /locus_tag="ACHKFK_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO9051188.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGISAAIGVVLGVLLSRR"
     gene            63709..64113
                     /gene="yqjE"
                     /locus_tag="ACHKFK_000775"
     CDS             63709..64113
                     /gene="yqjE"
                     /locus_tag="ACHKFK_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9051189.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLVIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            64103..64402
                     /gene="yqjK"
                     /locus_tag="ACHKFK_000776"
     CDS             64103..64402
                     /gene="yqjK"
                     /locus_tag="ACHKFK_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO9051190.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            64589..64981
                     /gene="yqjF"
                     /locus_tag="ACHKFK_000777"
     CDS             64589..64981
                     /gene="yqjF"
                     /locus_tag="ACHKFK_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9051191.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITGYAGIQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGMNSLMFMKN
                     LTISGGFLLLAITGPGAFSIDRLLNKKW"
     gene            65051..66037
                     /gene="yqjG"
                     /locus_tag="ACHKFK_000778"
     CDS             65051..66037
                     /gene="yqjG"
                     /locus_tag="ACHKFK_000778"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO9051192.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     KGGFAAEKERYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQPKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            66320..66688
                     /gene="yhaH"
                     /locus_tag="ACHKFK_000779"
     CDS             66320..66688
                     /gene="yhaH"
                     /locus_tag="ACHKFK_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9051193.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(66722..>67174)
                     /gene="yhaJ"
                     /locus_tag="ACHKFK_000780"
     CDS             complement(66722..>67174)
                     /gene="yhaJ"
                     /locus_tag="ACHKFK_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO9051194.1"
                     /translation="VIAPDMHFRSSSEINSRKLYTLMNVYVAAPDHPIHQEPEPLSEV
                     TRVKYRGIAVADTARERPVLTVQLLDKQPRLTVSTIEDKRQALLAGLGVATMPYPMVE
                     KDIAEGRLRVVSPESTSEIDIIMAWRRDSMGEAKSWCLREIPKLFSGK"
BASE COUNT        16372 a        17615 c        17021 g        16166 t
ORIGIN      
        1 ggtaatgtcc atagctgaat taatattaaa ttcaaacctg tattttcctc atccatttat
       61 aaaggattat agtcatgtta aaaaaacatt gttatctatg ttcgcaaccg cattgttatc
      121 aggcgttgct tttaacgctc ttgctgacga tgctaatcag ggttcaggta aaattacttt
      181 taaaggtgaa gttatcgatg cactttgttc tattgctcct ggtgatgaag atcagacaat
      241 aaacctcggt gaagttgctg ataccgtatt aaaaagcggt cagaaatcac tgcctgtaga
      301 tgtcaccatt catttgcagg attgtatttt atctgacggc actaacactg ttgataaagt
      361 caaaatcacc tttagttctg ccagtgttga cgctaccgac tccaacctgc ttaaaaacac
      421 tctggaaggt aacatcggcg gcgcaactga tgtaggcgta cgtctggtga aatcagacaa
      481 caccaacgtg actcttggca ctccaatcac tatcaacttc ccgacgacta actcttacca
      541 ggagttgaac tttaaagccc gtatggagtc tctgggacgc accgcgaccc cgggtaacgt
      601 gcaggcacag gctaattacg tactcgacta caagtaatcg gtagtcaata cctacacaac
      661 gccaggcggt aaagccgatg aaatttcatc ggcaactttg ggccttttta gaaatggatt
      721 tttatgaaca ataaaaacac gttttcccgg gataagttat cccatgcaat taaaaatgcc
      781 ctgtctggcg ttgtgtgttc cctactcttc gttttgccag tccacgccgt agaattcaac
      841 gtcgatatga ttgacgcaga agaccgtgag aatatcgaca tctctcgttt tgagaaaaaa
      901 ggctatatcc cccctggtag atacctcgtt cgtgtgcaaa taaataaaaa tatgttgcca
      961 caaacgttaa tactggaatg ggtaaaagcc gataatgaaa gtggttcgtt actctgctta
     1021 accaaagaaa atttgactaa tttcggtctt aatacggaat ttattgaatc attgcaaaac
     1081 atagctggca gcgaatgtct cgatttaagc caacgtcagg agttaacgac acgacttgat
     1141 aaagctacga tgatattatc gctaagtgtt ccccaggcat ggttaaaata ccaggcaaca
     1201 aactggacgc caccagagtt ttgggatacc ggtatcgccg ggtttatcct tgattacaac
     1261 gtgtacgcca gccagtatgc cccacatcac ggagacagca cccaaaacgt cagctcctat
     1321 ggtacgttag gctttaacct cggcgcatgg cgcttacgta gcgattacca atataatcag
     1381 aattttgctg atggacgctc ggtaaaccgc gacagcgaat ttgcgcgaac ttatctgttt
     1441 cgccctatcc cctcctggtc gtcaaaattc actatgggcc agtacgacct gagctccaat
     1501 ctttacgata ccttccactt tactggcgca tcgctggaaa gtgatgaaag catgctgccg
     1561 ccagatttac agggttatgc gccacaaatt accggcatcg cgcagaccaa cgcgaaagta
     1621 actgtggcac aaaatggtcg tgtactttat caaaccactg tcgcgccagg cccttttact
     1681 atttctgatt tggggcaatc gtttcagggg cagctggatg tcacagtgga agaagaagat
     1741 ggccgcacca gcaccttcca ggttggctcc gcatccattc cctatttaac ccgtaaaggg
     1801 caagtgcgct ataaaacgtc actgggaaaa ccgacatccg tcgggcataa cgatatcaat
     1861 aatccctttt tctggacggc ggaagcctcc tggggctggc tgaacaatgt gtcgttgtat
     1921 ggtggtggca tgttcaccgc tgatgattat caggctatca ctaccggtat tggctttaac
     1981 cttaaccaat tcggttcgct ttcttttgat gtcaccggag cagacgcgtc tttacagcaa
     2041 caaaatagcg gcaatctgcg tggttacagc tatcgcttca actatgcaaa gcatttcgaa
     2101 tcgacaggca gtcagattac cttcgcgggt tatcgcttct cagataaaga ttacgtgtcg
     2161 atgagtgagt acctcagctc gcgtaatggc gatgagtcaa tcgataatga aaaagagagt
     2221 tatgtcattt ccttgaacca gtactttgaa acgctggaat taaactctta tctcaacgtt
     2281 acacgcaata cttattggga cagcgccagc aataccaact actccgtatc tgtaagcaaa
     2341 aactttgata ttggcgattt caaaggtata tctgcatcgc tggcagtaag tcgaatccgc
     2401 tgggatgacg acgaagagaa tcaatattac ttctctttct ctctaccttt acaacaaaac
     2461 cgcaacatct cctacagtat gcagcgaacg ggaagcagta atacttcgca gatgatttcc
     2521 tggtacgatt catcagatcg caacaatatc tggaatattt cagcgtcggc aacggacgac
     2581 aatatacgtg atggcgaacc aacactgcgc ggcagctacc agcactattc gccgtgggga
     2641 cgtctgaaca ttaatggcag tgtacagccg aatcagtaca attctgttac cgcaggctgg
     2701 tacggttcac ttaccgctac acgtcatggt gtcgcccttc acgattatag ctatggcgat
     2761 aacgcccgca tgatggtcga taccgatggc atctccggca ttgaaatcaa ctctaaccgt
     2821 accgttacca acgggctggg catcgccgtg ataccttcgt tatcgaacta caccacctcc
     2881 atgttgcggg tgaacaataa cgatctgcca gaaggtgtcg atgtcgaaaa ctcggttatt
     2941 cgcaccacgc tcacccaggg tgccatcggc tacgcaaaac tgaatgccac caccggttac
     3001 caaatcgtcg gcgttattcg tcaggaaaat gggcgcttcc ctccactagg tgtgaatgtc
     3061 acggataaag cgacaggtaa agatgtgggc ctggtcgcgg aagacggctt cgtttatctc
     3121 agcggtattc aggaaaatag tactctgcat ttaacctggg gtgataatac ctgtgaagtc
     3181 acgccgccaa accaaagtaa cattagcgaa agcgcaataa ttttaccttg taaaacagtc
     3241 aaataattca ggatgaatat aatgaaatca ccattaatca caacaggaat gatttgttta
     3301 ctggggatct gcaactttgc ccaggcgact gtctcccccg acagaacacg catcattttt
     3361 aatgcttcga ataaaagcgc aacggttcgc ctgaccaacc aaagtaaaat cgatccctat
     3421 ctggctcagt cgtggattga agatgccagc ggtaaaaaaa cgcgtgatta catctcgaca
     3481 ttgccgccga tggaacgtat tgagcctgat gaacagatac aaattcgcct gatggcactg
     3541 gcctcgttaa acgacttacc acaggaccga gaaacgttgt tctactataa cgtgcgagaa
     3601 attccgccgc gcgccaaaga acagaatgta atgcaaatcg ccatgcaaag ccgcctgaag
     3661 ctattctggc ggccaaaagc gattgagtta aaagaggggg agatgattcc tctgcaaaaa
     3721 gtaacgatta cccgcaccgc cgccggtctg accctgaata acccgacgcc ttatcatatt
     3781 accgtcggtt atatcggcac taatggcaag acactgatgc ccggtgcgga cagtattatg
     3841 gtggcaccat ttacttctgc aactcaacac ctaagcagcc tgccctctac cttccagtta
     3901 ggctttgtgg cggactacgg cgggctggag atgtttaaag tcgaatgtaa ttccatccaa
     3961 tccctctgcc aaagctctcc tgctaagaag gggaaaatat gattacgctt tttcgtttac
     4021 tggcgattct ttgcctgttt tttaatgttt cagcttttgc tgttgattgc tatcaggatg
     4081 ggtacagagg aacaaccctc ataaatggag atttaccagc gttcaaaatt ccagagaacg
     4141 cgcaacctgg gcaaaaaatt tgggagagtg gagatattaa tatcacggtt tattgtgaca
     4201 atgcaccagg atggacaaca gacaatccat cagaaaatat ctatgcctgg atcaaattac
     4261 cccaaataaa tagtgccgac atgttgaata atccatattt aacctttggc gtgacttata
     4321 atggtgtaga ttatgaaggg ataaatgaaa gaattgatac tcatgcatgc ctggataaat
     4381 atgagcaata ctataatggg tattattatg accctgtatg caatggcagt acccttcaaa
     4441 aaaatgtaac atttaatgcc cattttcgcg tctatgtaaa attcaaaagc cgcccagcag
     4501 gagatcagac ggtgaacttt ggcatagtca acgtgttaca attcgatggc gaaggagggg
     4561 caaacatgtc ctctaacgcg aagaatttac gctatgtgat tacgggatta gataatattt
     4621 cattccttga ctgtagtgtc gacgtccgga tttttccaga aagccagata gttaatttcg
     4681 ggcaaattgc tgcaaactct attgctactt atcgaccaaa agctgcattt agcgtatcta
     4741 ctataaaaga tgttgctgcc gattgtaccg agcaatttga tgttgccacc agcttttata
     4801 cgacagatac gttacatgat gatacgcatc tggaaatggg taatggtttg ttgatgcgta
     4861 ttactgatca aaaaacacaa gaagacatta agtttaatca gtacaaacgc ttcaccacat
     4921 atattccagg tcagactgga gcgatggtta cccgtgatta ccaggctgaa ctttcacaaa
     4981 aaccggggga gactctcgtt tatgggcctt tcaaaaaaga tctcatcgta aaaataaact
     5041 ataactgaac agcaaaatgc cgggaacacc ccggcatttt catatttagt atttcacgtc
     5101 attagctggc tttacgctca tgtgcctgac ggtatgccac caggtcttca atagtcacga
     5161 gcgccatatt gtgtttattg gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat
     5221 cgtcattagt cagctcacac agcacaccag ctggtttaaa gcctgccagc gtcatcagat
     5281 cgatagtcgc ttcagtatga ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg
     5341 ggaaaacgtg gcccggacga ttcagatctg aaggttttgc gccatcggca atcgctgcgc
     5401 gaacggtcgt aatacggtca gcggcagaaa caccggtagt cacaccttca gctgcttcaa
     5461 tggtcacggt aaaaccggtg ccataggcgc tggtgttatt ttctaccatc attggcagat
     5521 cgagttgttt acggcgatcg tcagtaatgc acaggcaaac aataccactc ccgtggcgaa
     5581 tggtcagcgc catctgctca acggtcatgg tttctgccgg gaagatcata tcgccttcgt
     5641 tttcacggtc ttcatcatca agcaccatca caccgcgtcc ttcacgcagc gcagccagtg
     5701 cattttcaac acgttcgaaa ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa
     5761 aaaaacctca ctaaaattat ggttaccaga atcagggcag tcttaggagt ggcggcatat
     5821 agccaaaata acgtgagcgg gtccatgccc gacagaatcg ttactctctc ccatccggac
     5881 tctaaccgtc ggccccggaa ttacaccgga tctgctgtcc tttgagttcg cacccaaagc
     5941 gctcgcgggc tttcaactga gttgatttac cgccggtggg gaatttcgcc ccgccctgag
     6001 aataagcgga ttcactataa cgctaatgat tagcgccagc aacgcatagc ttcacataat
     6061 tctggtttat gacttaccct tatcgcacta caatggcact caacacctat cagtacaggg
     6121 aaactacaat gattgacccg aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa
     6181 tgcctaaagg aatcagggag ttcggggaag atgtggagaa aaaaatccgc caaaccctac
     6241 aagcgcagct gacgcgtctc gatctggtaa gccgcgaaga attcgacgtt cagacgcaag
     6301 tcctgttacg gactcgtgaa aaactggcgt tactggagca gcgcatcagc gaactggaag
     6361 cgcgcaataa tccggtagca gaacttcaat ctcctccggc tatcccgcca gttgataaag
     6421 cagaataaag caccacaaaa acgggccaga cggcccgtaa tatcagtgtt ctaattcaag
     6481 ttctctggtt tgcatcatct gttggcaata ccaggcataa cgtgcgcaaa accaggttct
     6541 atgttcttca tccatgttac cagtaacggc cagaatatcg accgggcgac cttctgcgtg
     6601 cattctggcg aaactacgcg cgagaaaatc gaaattattt aaagaattaa gactatgatc
     6661 cataagcaca tcctccggtt attgcatacc ttttaaggcc atatgctttt tctgatttca
     6721 gtattcccca atcagtgtag tgcctttaaa cataacgtgc gtaaatatat tgtccccgat
     6781 cacacttttt agtgaaaaat aaaaaaacca tattaaaaga ctttctcttc cagaacctct
     6841 ctaacatgtg ttattattaa caaagataaa tgtaagcaag gaaactattc caaagtaaat
     6901 attgcgtcac taaatggaca ttggagtgac atatgatttt attcgccgac tataacaccc
     6961 cttatctttt cgctatttcc tttgtactct tgatcggcct tttagaaatt tttgcactta
     7021 tctgcggtca catgctctcc ggtgctcttg atgcacatct tgaccattat gattccataa
     7081 ccacaggtca tattagccag gcacttcatt atctcaatat tggaagatta ccagctctcg
     7141 tcgtcctctg cttactcgct ggtttttttg gtcttattgg catcctgttg caacatgcct
     7201 gcgttacgct ctggcaatca ccactcccca acctgttcgt ggttcctgtc agtttactgt
     7261 ttacaattat tgcagtgcat tataccggta aagtcgttgc cccctggatt ccacgcgacc
     7321 atagttcagc tattacagaa gaagaatatg ttggtagcat ggcattaatt accggccatc
     7381 aagccacttc aggtaaccct tgcgaaggaa aactcaccga tcaatttggc caaatacatt
     7441 atttgctact ggagcctgaa gaaggaaaaa ctttcactaa aggagacaaa gtgttaatta
     7501 tttgccgact ttctgcaacg cgatatttgg cggaaaataa tccctggccc cagatattgt
     7561 gatttattaa tcaaaaggaa atttaataat ggatgatatc gttaattctg tgccctcctg
     7621 gatgtttacc gcgattatcg ccgtatgcat tctgtttatt attggaatta ttttcgccag
     7681 gctctatcgt cgcgcttcgg cagagcaagc atttgttcgt actggtttaa gtgggcaaaa
     7741 agtggtgatg agcggtggcg caatcgtgat gccgatcttt catgaaataa ttcccatcaa
     7801 tatgaatacc ctgaagctgg aagtcagccg ctcaaccatt gatagcctga ttacgaaaga
     7861 tcgtatgcgc gtcgatgtcg tggttgcttt ctttgtgcgg gtaaaacctt cagtagaagg
     7921 catcgccact gcggcccaga cgctggggca acgcactcta tcaccagaag atttacgtat
     7981 gctggtcgag gataaattcg tcgatgccct acgcgccacc gctgcgcaaa tgaccatgca
     8041 tgagttacag gatacccgcg agaacttcgt gcagggagta caaaataccg tagctgaaga
     8101 tctgtcgaaa aatggcctgg aactggaaag cgtttcactt accaacttta accagacgtc
     8161 gaaagaacat ttcaatccta acaacgcctt tgacgccgaa ggtttaacca agctgacgca
     8221 ggagacggag cgccgtcgtc gcgaacgtaa cgaagttgaa caggatgtag aagttgcggt
     8281 gcgtgagaaa aaccgtgatg cgctttcgcg gaagctggag attgaacagc aagaagcgtt
     8341 tatgacgctt gagcaggagc agcaggttaa aacccgtacc gctgagcaga atgcgaaaat
     8401 tgcggctttt gaagctgaac gtcgtcgtga agcagagcag acgcgaattc tggctgaacg
     8461 acagattcag gaaacggaaa tcgaccgcga acaggccgtc cgctcaagaa aggttgaggc
     8521 cgaacgtgaa gttcgcatta aagagatcga acagcagcag gtcaccgaaa tcgctaacca
     8581 gacgaaatcg atcgctattg ccgccaaatc ggaacaacag tcccaggcag aagcgcgtgc
     8641 taaccttgca ctcgcagaag cagtaagcgc ccaacaaaac gtagaaacca ctcgccagac
     8701 tgccgaagcc gatcgtgcta aacaagtggc gttgatcgcc gcagcgcagg atgcagaaac
     8761 caaagcggtt gaactgaccg tgcgggcgaa agcagaaaaa gaagccgcag aaatgcaggc
     8821 ggcggctatc gttgagttag ccgaagctac acgtaaaaag ggtctggcgg aagcagaagc
     8881 acaacgtgcg ctgaacgatg ctatcaacgt actttctgat gaacaaacca gccttaaatt
     8941 caaactggca ttattgcagg cgctgcctgc ggtaattgag aaatccgtgg aaccaatgaa
     9001 gtctatcgac ggcatcaaaa ttattcaggt cgatgggctg aatcgtggtg gcgctacggg
     9061 tgatgcaaac acgggtaatg tggggggcgg aaacctggca gaacaagcgt tatcagccgc
     9121 actctcttac cgtacacagg caccgctgat tgactccttg ctcaatgaaa ttggcgtttc
     9181 aggcggctca ctggcggcat tgacttcatc cttaagctca acaactcccg tcgaagaaaa
     9241 agcagagtaa accctcttct atgaataatc cctggcccaa aaagccgggg attttttata
     9301 tctgcgttcc gctaaaaggt gcaaatgatc aagctgttgc agcattttgc gtgactgctc
     9361 ggggaaggca aaactgcctc tgggaaagcg ttgcgcgggg tccggcgctc atcaacaatc
     9421 ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc cttgtataag
     9481 gaaagggtta tgatgaagct cgtcatcata ctgattgtgt tgttactcgt aagtttcgca
     9541 gcttattaac agccaatcag aggggggaga aatcctccct cacccttgtt cctttacttt
     9601 aggttgaaaa aaaatcagcg tcaataggcc cgcctgctac aaagcgagat ctgtgaaccg
     9661 ctttccggtt agcctttttt atcctgttgg atcttcttga tgatgttggt cgtcgagcaa
     9721 ccgtcttcaa agttgagcac cagcacttcg ccaccgttgg cccagacttc tttactcccg
     9781 gcaatctctt ctggtttata gtcgccgcct ttcaccagta gatctggcaa gatcccggcg
     9841 atcaagcgct gcggcgtgtc ctcttcaaac gacaccaccc agtcgaccgc ttccagtgcg
     9901 cccagcacaa tcatacgctg ttcgagcggg ttaacggggc gggaatcccc tttcagccgt
     9961 ttggtggagg catcgctgtt cacagcgaca atcaaacggt cacccagttt acgggcattt
    10021 gccagataag agacgtgccc agcgtgcagg atgtcaaaga caccgttggt catcaccact
    10081 ttttcaccgc gtttacgcgc tgccgctaca gcaagcttca gttcctcttc ggtcatcaca
    10141 ccaaagccgg tatctgcacg tccacgtaca gcgttttcca gctcgatcgg cgaaaccgtg
    10201 gatgtcccca gtttgccgac caccacacca gccgccgcat tggcaaagaa gcaggcttct
    10261 tccagtgaat tacccgctgc cagcgttgcc gccaggacgc caatcaccgt gtcgcccgca
    10321 ccggtaacgt catacacttc ctgcgcctgg gttggcatat gcagtggcgc tttacccggt
    10381 tgcagcagcg acataccctg ttcggaacgg gtcactaaca gagccgagag ttcgtaatcg
    10441 gcaatcagtt tcatgccgcg ctcaacaatc tcttcttcgg tcttacattt accgacaaca
    10501 gcttcaaatt ccgagagatt cggcgttaac agcgtagcgc cgcggtagcg ctcaaaatcg
    10561 gtaccttttg gatcaatcag caccggaaca cccgctttac gcgccagttg gatcatctgc
    10621 tgtacgcttg ccagcgcacc tttggcgtag tcagaaagca ccagcgcgcc aatcgaactc
    10681 agtgcctgat taatccgctc gtgcagcggc tgcggatcaa ccccttcgaa accttcttcg
    10741 aaatccagac ggatcagttg ttggttgcgg gaaagtaccc gtaatttggt gatggtcgga
    10801 tgcgtcggta cagaaacaaa gtcgcatttg acgttgacgt cggccagaga tttactgagc
    10861 gcgcgcgccg catcgtcaat gcccgtcaac ccgaccaggc gtgcattagc accgagagaa
    10921 gcgatattca tcgccacgtt agccgcgccg cccggacgtt cttcgatggt attcacttta
    10981 accacgggca ccggcgcttc cggcgagata cggctggtgg gaccgtacca gtaacgatcc
    11041 agcatcacat caccaaccac catcactcct gcacgttcaa actctggcag cgttactttc
    11101 attcctgtct cctgagagat tcaaaatttg cgcgcgataa taccatactt cattcttcca
    11161 ccagccactt ctgccagctt gcccgcacca gttcacgctc tgcggtgaag caattctccg
    11221 acacatgacc cggcaattcc tgtaatgcca gatgatgaag ttcatcgcgc agcgtcgtgt
    11281 aagcacgggt cagcgccatc gcttcctgct cttccataat gtcgttttgc gccagtagtt
    11341 ccagaatgcg cacgttgtct gaccagcgcg ttaacttcgg tttttcgtga gcgtagcgca
    11401 acaccagata ttgagtaata aattcgatat cggtaattcc gccttcatcg gctttgatat
    11461 caaagcgatc gcgatgttta ttgccgagat gggcacgcat tttctcgcgc atttcccgca
    11521 cttctgtttg cagggttttg ccttcgcgcg gcagcgtcat aatctcccgg cgtactgcgt
    11581 caaagtgcgc ggtgagctgc ggatcgccgt acactacacg cgcacgcacc agcgcctgat
    11641 gctcccacgt ccaggcctcg tttttctgat aatcagcaaa tgcttctgcg gatgtcacca
    11701 gcattcccgc cgccccggac ggacgcagtc gtgcatccac ttcatacaaa atgccggaag
    11761 aggttcgcgt actgaacaga tgcataatgc gttgcgccag acgcagataa aactgtcgcc
    11821 cgtcgatttc ccgctcgccg tcagtcatcg catccattgg gcaatcatgg aggaagatca
    11881 ggtcaagatc ggaactgtag cctaactccc agccacccag cttgccgtag ccgaccaccg
    11941 caaaaccacg cccttcgcgt tcgttcaggt gatttggctt accgtagcgg gcaaccattt
    12001 gaacccacgc ctgctgaacg acggcatcaa tcatggcttc tgccagccag gttaagtgat
    12061 cgctcacttt cattaccggt agcgtaccgg cgatatccgc tgccgcaatg cgtaacagct
    12121 gcgcctgttt gaactgacgc agcgcctcaa gctgttgttc ttcgtcatct tccggcacgc
    12181 gcagcaaata ctggcgcagt tcatcacggt aggcatccgt cgccgttggt tggtagaggg
    12241 tatttggatc gagcaattca tccagcaata atggataacg cgctagctgg ctggcaatca
    12301 tcggcgatgc agcacacaga gaaatcaaat gtttaagcgc cgcagggaat tcactaagca
    12361 gctcaagata agtggtgcga gtaacaatcc ccaccagcaa ggcggtaatg cgcgacagcg
    12421 tgacagcagc gtcttcacgc gcacagacat cacttagcag atgtggcatc agatggtcga
    12481 gcacctgacg tcctcgcggc ccgatggtgc gcttatccag ctctttgcgg aaatcagcaa
    12541 tcagcattag cacctgtttg cgatcatcct cgctaagatg cgccagcact ggcgtggtgt
    12601 catcttcctg caacgcatcc tgccacagct cacgccactg ttccgacagc gactcttcct
    12661 gagtttcact ttcgtcgtcg ccaatcaatt cattaaacac ccggcgcaca ttggccatat
    12721 gtgcggtcag caccccggtc aattgcggcc agtcagcaaa gtccatcgcc cacgtcagcc
    12781 gcgcacggtt aagctcatca aaaggaagcg tctgggtttg ttcgtcgtta atgctttgca
    12841 gcaggttttc cagacgccgc aggaacagat acgccactcg caattgttca gcatcgtttt
    12901 cagaaagcag atgcagcgcg gcaatggcgc tgagcgttgg cagtaaagag cgcgattgca
    12961 gcgacggttc gcgcccgccg cgaatgagct ggaacacctg gacgataaat tcaatttcgc
    13021 gaatgccgcc tgcgccaagt ttgatgttgt cggtcaaacc gcgtcgacgc acttcacggg
    13081 caatcattcc tttcatgttg cgcagcgact gaatcacgct gaaatcgatg taacgacgga
    13141 aaacaaacgg acgcagcatc gcacgcaact cgttagcata gacgccgtcg ctatccccca
    13201 taatccgcgc cttgaccatc gcgtaacgct cccagtcgcg cccctgctcc tggtaataat
    13261 cttccagcgc cgcaaaactc agcaccagcg ggccactttc accaaacgga cgcagccgca
    13321 tatccacgcg atagacgaaa ccatcctgcg ttggttgatc cagcacttta atcagccgct
    13381 gccccatgcg ggtaaaaaac tgcgcgttat ccagttcccg gcgtccgccc tgggtacaac
    13441 catgttccgg ccaggcaaaa atcagatcga tatctgagga gaaattcagt tccccaccgc
    13501 ccagcttacc catgcctaaa atcagcagcg gttgcgcttc gccctgcgca ttgcacggcg
    13561 ttccccattc gcggcagcag gcgtcataca gccagtcacg cgccgcaaca atcagcgtct
    13621 ccgccagatg gctgagctgc tgcaatatgc tctcttcagt aaccagtgcc agcgtttgcg
    13681 cccaggcgat gcgcaccata atgcgccgcc ggaatagccg cagctcgcgc attaacccgg
    13741 cttcgtcact cacattactg agcgcctcct gcaaccatgc tgcgtaatgc tgccattcgt
    13801 cggcctgcgg cggttggctt tccagttccg tcagccactc tggatgcgca attatgctgt
    13861 cctgcagaaa atcactgaaa gtaagtactg acttcgcctg tgcgctaagt gattcctcgg
    13921 ctaaaggctc tggcagccgc tcaacaacgg tctgccagta ctgctgtaac ggtgaagaga
    13981 gcggcttcat aagcgatttt atccttgcct gattaacgtt ttccgctgtg caaccagaac
    14041 ggttcctgat tgtttgcctc attacggaaa tgttcaattt caatgcgttg cccggtcgca
    14101 atagcgtgac gcagcccctg ccagttctcc agccagtctt gcgcgacgac aggatcgtag
    14161 taacccgcca gcaacagtat taagtcaata tcacgcgtca ggcgtggcaa ttggtcacgg
    14221 tagcgatcgc ctaacggctg gcagaaaacg cttttcagtt cagcggcatg gcgggaaaga
    14281 tggatatcgg caaagcgttt gaaggaatcg ctgattttgc tctgcgcttt ggcatctaaa
    14341 aacggctgcc aggctttgct taccagccat tcggtcaacg ccagcttcgc cattgccgtt
    14401 tcggtagagt aaaccgccgt tacggcagaa accgcagaag caatggtcgc ctcgcattga
    14461 gtcagcagat cacgtaagtg agtgctcgct ttacgcggca cgataccgcc gaacagcatc
    14521 agtgtatgac ggaccaggcc aatggctgcc agcacctgct ctttcgccgc atcgttgccg
    14581 cgtacccaca gttcttcatg atactgccat tgcgctaacg ccagctcgaa cgccgcttcc
    14641 agcccctgtt ccacatcagc ttttgccgca acatgcaaaa tggtggtcgg tttgatttca
    14701 cgcgccggat tgccctgcgc cagatgatag ccacgcgccg ctttgctcag gctgccctgg
    14761 cgtaatccgg tttgcgatac cagttggttc gccagtttca gcaccgcgcg cgtgtcgccg
    14821 ctaagcagtt ccagttccag ctcgcagata ggttcagcaa attcacccgc tttcacttcc
    14881 ccctggtcga gggcgatttc aatttggcta ccatcaacct ctaccagcca cttttcgcga
    14941 taaaaatcgg tgctaaacag cggctgcacg cgggaagcga gatcggtagg caattcgccg
    15001 ttcggccaga cttccgtcgg taactgcgcc aggtcgagcg tcggttcgct caacgccacg
    15061 ttatattccg ggcgctggtg taagccgcct gtcactcttc ctgcaacttt catggtcatc
    15121 tcatagcgac cgttttcgcc acgaatacgc aagcccatgt cgtgcccacg cagccagtta
    15181 tccggcgttt cgtagtaaat attcagcaac tgcacgggat catggtgctc gccgcccagc
    15241 gtattgagat ggtcacgcaa cgcctcaacg gcactgtgat taacaataaa ctttaattcg
    15301 atttcctgag ccatagcctt tacttatcgg ttatgtcaca taagtgacga tgaacggcga
    15361 acttaatgcg atcttttttg tcagtagata gtattttgcg ccaaattgcc atgcaacggg
    15421 caatttgacg ggcgtaaaag tttgaagcag tggcaagacc tgacacagag gattccgatt
    15481 gatgtcgaat gctttgcgta gcgacactga tgccactact atcgttccac tttccatgac
    15541 aataacgaca gcctgatgcc aaaattacgc ctgatcggat taactttact cgcacttagc
    15601 gcgactgccg tctcacacgc tgaagaaacg cgctatgttt ctgacgaact gaatacctgg
    15661 gtccgtagcg gtccgggaga tcattatcgc ctcgtgggca cggttaacgc cggcgaggaa
    15721 gtgaccttat tacaaactga cgccaacacc aattatgccc aggtgaaaga cagctctggc
    15781 cgtaccgcct ggatcccgtt gaaacaactt agcacggagc caagcctgcg ttcccgtgtg
    15841 ccagatctgg aaaatcaggt caaaacgctg accgataaac tcaccaatat cgataacacc
    15901 tggaatcagc gcaccgcaga aatgcagcaa aaagtggcgc agagtgacag cgtgatcaac
    15961 gggttaaaag aagaaaatca gaaactgaaa aacgagctga ttgtcgcgca gaaaaaggtc
    16021 gatgccgcca gcgtacagct ggatgacaaa cagcgcacca tcatcatgca gtggtttatg
    16081 tatggcggtg gcgtgctggg gcttggcttg ctgctcggtc tggtactgcc gcacctgatc
    16141 ccaagccgca aacgcaaaga tcgctggatg aactaaatcg ccttcttcgt tgcactgtct
    16201 tattattagg aattgtgtat ttgacgaagg gatgatggcg tgaagattta tctggtcggt
    16261 ggtgctgttc gggatgcatt gttagggcta ccggtcaaag acagagattg ggtggtggtt
    16321 ggcagtacgc cacaggagat gctcgacgcg ggctaccagc aggtaggccg cgattttcct
    16381 gtgtttctgc atccgcaaac gcatgaagag tatgcactgg cacgtaccga acggaaatcc
    16441 ggttccggtt acaccggttt tacctgctat gccgcaccgg atgtcacgct ggaagatgat
    16501 cttaagcgtc gcgatctgac cattaatgcg ctggcccagg acgataacgg cgagattatc
    16561 gacccgtaca acggtctggg cgatctgcaa aatcgtctgt tgcgccatgt ttcccccgct
    16621 tttggcgaag atccgttacg cgtgttgcgc gtagcgcgtt ttgctgcgcg ttatgcccac
    16681 ctctgtttcc gtattgccga tgaaactctg gcgctgatgc gcgagatgac ccatgcgggt
    16741 gaactggaac acctgacgcc tgaacgggta tggaaagaga cggaaagcgc cctcaccacc
    16801 cgcaatcccc aggtgttctt ccaggtactg cgagattgcg gcgcactgcg cgttttattc
    16861 ccggaaattg acgccctgtt tggcgttccg gcccctgcca ggtggcaccc ggaaatcgac
    16921 acgggtattc ataccttaat gacgctctca atggcggcga tgctgagtcc gcaggtcgat
    16981 gtccgtttcg caacgttatg ccacgatctc ggtaaaggac tgacgccgcc agaactttgg
    17041 ccacgtcatc atggtcatgg cccggcgggt gtgaagttag tggaacaact atgccagcgt
    17101 ctgcgcgtgc caaatgaaat tcgcgattta gccagactgg tagctgagtt tcacgatctc
    17161 atccacacct ttccaatgct gaacccgaaa accattgtta aattgtttga ttccattgac
    17221 gcctggcgta aaccgcagcg tgtcgagcaa ctggcgctga ccagcgaggc tgacgtgcgc
    17281 ggcagaaccg gttttgaatc agcggactac ccgcaaggcc gctggttgcg cgaagcctgg
    17341 gaagtggcgc agtcagtgcc gacaaaagcc gtcgttgaag caggatttaa aggtgtggag
    17401 attcgtgagg agctgacccg acggcggatt gcggcggtag ccaactggaa ggaacaacgc
    17461 tgcccaaagt ctgagtaatt gttggatatt ttgccggatg cggcgtaaac gccttatccg
    17521 gctacatatc aacgacggtt gtaggcctga taagacgcgc aagcgtcgca tcaggcgttg
    17581 attgtcggat gcggcgtgaa cgccttatcc ggcctacgca atcgaggaca attccaggca
    17641 tccgacacta ttcttcacat caaaagaaca cgacgtacac cgcagccgcc acaataaagc
    17701 gataaatggc gaatgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg
    17761 ctatcagcgc caccacaaaa gcggtgataa acccaacggc aaacatcggg atatcgccgg
    17821 tagtcaggaa gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggca
    17881 ccgccagcag gaacgagaac tcggaagcag cgtaacggct cacccccatc agcatcccac
    17941 ctgaaatggt tgccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc
    18001 caatcataaa cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg
    18061 gcttcaggca ttcggcggca atcagcaaca gaccgccaac gaccagtgca tacatgacat
    18121 ttatcgggtt aaacaatgat ttaatcgtgt cgtggaacaa cagccccagt accaccgccg
    18181 gaatcatccc cagcaagatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct
    18241 gcaacgggcg accaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta
    18301 ctaccgccag aattgatcct aactggatca cgacttcaaa ggttttcgcc gtgtcgccct
    18361 caaaccccaa caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt
    18421 ctgtcaatcc ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca
    18481 tcaataaacc cctaaattat aaaaatgtaa cgcttgtccg aactactgca gtatgaccag
    18541 gttataatcg tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac
    18601 gctcaatgat tacgccaaca ttcgccgccc gcgccactgc gcctggcttg ctgagtttga
    18661 tacgcaccca cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc
    18721 gttccaccag cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag
    18781 cgtaactgag gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccacgcca
    18841 tttcgatatc gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag
    18901 tggtgattac cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg
    18961 gctaaccgga taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca
    19021 cgaattttca aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg
    19081 tacctctgcg gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gttgcccgat
    19141 ccgcgaacca gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag
    19201 ggagcagccg tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc
    19261 gcgtatgaat taggtgtcag ccccttctgg ctaggcttaa ttgccatcgc cgcctgtctt
    19321 ggacacatct ggcccgtttt cttcggtttt aaaggaggaa aaggcgttgc taccgctttt
    19381 ggtgccatcg caccaattgg ctgggatctc actggagtaa tggcgggaac ctggttactg
    19441 accgtgctat tgagtggtta ctcgtcgctg ggagcgattg tcagtgcact gattgctccg
    19501 ttttatgtct ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg
    19561 atcctgctgc gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc
    19621 tggacgaaat tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat
    19681 gatctgatag ccttcgtcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc
    19741 cacacagaca cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt
    19801 ggcgctttgt gcatactccg gcaatactcg caccagtttg ccactctcca gaaacggcaa
    19861 cacatcccac tcggaacgca gcataatccc tttgccctcc agcgcccatt gcagcacaat
    19921 ctcgccgcta ttggaggaaa gatgtccgct taccttcacc gattttttct cctgaccgtt
    19981 ccccaactcc catattccgt gggtcatatc gcgctctttg gtcaccaggc agtcatgacg
    20041 acttaattcc tgtaaggatt gtggctgcgg atatttttgc agatactcag gtgctgcgca
    20101 taatattctt ttattttttg tcaaaagatg cgcaatataa taatcaggaa ttgcgtcatt
    20161 aatacgaata tccagatcaa tattatcctg cactaaatca atttgtcgat cgaacaattc
    20221 aaaatgcacc tgtaactcag gataattgcg catcagttcg gtaatagctg gcgcaatatg
    20281 gctgcgccca aaaccaaaac tgcaaccaat gcggatcatc ccctccgggc gcgttttgat
    20341 ttgcgtgacg tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta
    20401 acaacgctgg ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagagttgt
    20461 tgccagcgta ttttcaagga tttggatccg cttggtgaca aatgcaggcg tttgcccaag
    20521 cgtcgccgct gcagcactaa aactcccgct atggactatt tccaccagta cctgcaagtc
    20581 tttggctaaa ggccagctat ttagcattgt taaatatccg cagttttgat aaacgcagtg
    20641 tatttcgcaa aacattacca ttccctgctc tggcgcaata ttcgttctta ttttcgcgtt
    20701 gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag
    20761 tggtagtttg tggcggtgaa tttttccaga caaatacata aactggagtt gccatgatga
    20821 gcgaaagtaa taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg
    20881 ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg
    20941 ccgaaacatc gtcgatggga aaaatcatct accacacgat gttcgataac atgcaaaaag
    21001 cgatcgacct gaatcgtcct gcctgtcagg acaccggcga aatcatgttt tttgttaagg
    21061 tcggttcccg tttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag
    21121 aggcaaccgt caaagcgcca ctgcgtcaca atgcggtaga aatttttgac gaagtaaaca
    21181 ccggcaaaaa taccggcagc ggcgtaccgt gggtcacctg ggatatcgtc cccgacggtg
    21241 acgatgcgga aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgatcga
    21301 aagtgttaat gccgtcagaa ggctacgaag gcgtagtgaa attcgtcttc gaaaatatct
    21361 ccaccctcgc cgtaaacgcc tgtccgccgg tacttgtggg cgttggcatc gctacctcgg
    21421 tggaaaccgc cgcagtgctc tcgcgaaaag ccattttgcg cccgattggc agccgccacc
    21481 ccaatccgaa agcggcagag ctggagctac gcctggaaga aggactcaac cgtttgggga
    21541 ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtgcatatc gaatctgccg
    21601 ctcgccatcc gtcaaccatc ggtgttgctg tttctaccgg ctgctgggcg catcgtcgcg
    21661 gcacactgcg agttcatgcc gatctcacct ttgaaaatct gtctcatacc cggagcgcgt
    21721 tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcgg
    21781 cgatgtgatc tacctgatcg gtacgctggt gacctgccgt gacgtttgtc accgccgatt
    21841 gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg
    21901 ccccatcgtg cgcaaaaacg gcgacaaatg ggagatggtg tccgtcggcc cgacaaccag
    21961 tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt
    22021 cggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctgca
    22081 tgtgattttc ccggctggct gtgcggtact ggcggcaact caagtggaag agattgaaga
    22141 ggtacactgg acagaactcg ggatgccaga gtcgctgtgg gtctgccggg tcaaagagtt
    22201 tggcccgctg attgtctcta tagataccca cggcaacaac ctgatagccg aaaacaaaaa
    22261 gctgttcgcc gaacgccgcg atcccatcgt ggaagagatt tgcgagcacg tccattacat
    22321 caaataaccc tcacggagag gctcgcccct ctcctttttc tcaggcataa cacgatgaaa
    22381 ccttccactg aatggtggcg ataccttgcg ccgctggcgg tcatcgccat tattgctcta
    22441 cttccgcttc ccgcagggct ggagagtcat acctggctct actttgccgt ttttactggc
    22501 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtaggtata
    22561 tccatcatcg ccattctttc gccgtggctg ctgtttagcc cggagcagct cgcccagcca
    22621 ggttttaagt tcaccgccaa atccctctcc tgggcggttt ctggtttttc taattcagtt
    22681 atctggctta ttttcgccgc ctttatgttt ggtacgggct atgaaaagac cgggcttgga
    22741 cgtcgcatcg cgctgattct ggtgaaaaaa atggggcatc gcacgctgtt tctcggctat
    22801 gcagtgatgt tctccgaact gatactcgcg cctgtaacac cgtccaactc ggcgcgtggt
    22861 gcggggatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    22921 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgattgc
    22981 gtgaccagcg ccattttcct gacggcgatg gctccaaatt tgctgttaat tggactgatg
    23041 aaaagcgcat ctaacgccac gctgagctgg ggcgactggt tcctcgggat gttgccgctc
    23101 agtattttac tggttctgct ggttccctgg ctggcttacg tgctgtaccc gccgatactg
    23161 aagtctggcg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    23221 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cactggtgct gtggattttt
    23281 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcgct gatgctgctt
    23341 ctgcgcatta tctgctggga cgacattgtc agtaataaag cggcgtggaa cgttttcttc
    23401 tggctggcct cgcttatcac cctcgctacc ggactcaaca acaccggttt tattagctgg
    23461 tttggcaaac tgttagcagg cagcttaagc ggttattcgc caacgatagt gatggtagcg
    23521 ttgattgtgg tcttttatct gctgcgctac ttttttgcca gcgccacagc gtatacctcc
    23581 gctctcgcac cgatgatgat tgccgccgcg ctggcgatgc cggaaatccc gctgccggtg
    23641 ttctgcctga tggttggtgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    23701 ggacccagtc cgatttacta cggcagtggt tatctgccaa cggcggatta ctggcgactg
    23761 ggggcgattt ttgggctgat attcctcgta ttgctggtga ttaccggctt actgtggatg
    23821 cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca cccaatagtt
    23881 ttacgcagcc ggtaactccg ccagcggcca gcgcggacgc acactcacgc cgagatccgc
    23941 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcac cgttatcagt
    24001 gcaaaattcc ggacgcgcgt agaacacttc gccacgacgc tttttcatca tttcagccag
    24061 cttcgcccgc agtgtgcggt tagcactcac gccgcctgcc atgaccagtc gcttaaagcc
    24121 cgtctgatcc agtgctcgct tacacttaat catcaacgta tcgaccactg catcttcaaa
    24181 ggcgcgggcg atatcggcac gcgtctgatc gtcagtgccg ttatcacgaa tggtatttgc
    24241 cgcgaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcgggcg
    24301 cgggaagaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta atggcccgcc
    24361 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccag ccgcatcatc
    24421 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    24481 accgccggaa accagcagcg cgacaaacgg aaattccggt gggttatctt ccagcatcgg
    24541 cgctaacaga tgcccttcca tatggtgtac cgggatcgcc ggaacgtccc aggcaaacgc
    24601 cagagaacgc cccacggtcg cgccaaccag cagcgcgccg actaatccag ggcctgcggt
    24661 ataggccaca gcatcaatgt cttttgccgt taaacctgac tcctttagcg ccgcctggat
    24721 caacggtacg gttttacgca cgtgatcgcg ggaggccagt tcaggcacga cgccgccgta
    24781 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    24841 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    24901 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    24961 tcgcccctag agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    25021 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    25081 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    25141 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    25201 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    25261 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    25321 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    25381 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    25441 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    25501 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    25561 atttccacgc gtgttgtcca ttccacaacg agaaaacccc atcattcacc gttaacggtg
    25621 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcttga
    25681 tgaactacga caagctcgag ttcgtcgaaa cggtcgaaga gctggcagca atgcacaatc
    25741 ttgaagtgcc atttgaagca ggtagcggcc ccagccagat cgagcgccat caacggcaaa
    25801 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta cagcaacctg
    25861 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgccc
    25921 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    25981 atccagaaaa tcgccagtca ttgattgatg cgggcatgtt ggtcactaac gatcaggggc
    26041 gcagttacga ccgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg
    26101 tgattggttt tggcgggcgt gtgctgggca acgatacacc caaatacctg aactcgccgg
    26161 aaacggacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    26221 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    26281 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    26341 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    26401 gcgatgccgc ctggcgagcg ctggaaacgg cgctgcctta catgacagac ggtcgtcagc
    26461 tacgctttat gtttttgcct gatggcgaag accctgacac gctggtacga aaagaaggta
    26521 aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt ctgtttaaca
    26581 gtctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    26641 cactgccact gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    26701 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    26761 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    26821 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    26881 atgaaaataa gctccctggg cttggcttat tcagagaact ggtcaacact tgtctctccc
    26941 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    27001 cccttgaaaa actgtcgatg tgggacgata tagcagatag gaatattgct gagcaaacct
    27061 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    27121 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    27181 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    27241 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    27301 ggcggtaaac aaccgttgga tttcagcgtt aacagctgaa ggacatcggg tcaatcgccc
    27361 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    27421 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    27481 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    27541 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    27601 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    27661 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    27721 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    27781 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    27841 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    27901 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    27961 agatctggac gatgacgaag atgaagacga agaagatggt gacgacgata ccgccgatga
    28021 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactgc gcgctcagta
    28081 cattgtaacg cgtgacacca tcaaagcgaa aggccgcagt cacgctgccg ctcaggaaga
    28141 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    28201 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    28261 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    28321 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    28381 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga
    28441 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    28501 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttgc gtctggttat
    28561 ttctatcgct aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    28621 taacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    28681 cacctacgca acctggtgga tccgtcaggc gatcacccgc tctatcgcgg atcaggcgcg
    28741 caccatccgt attccggtgc atatgattga gactatcaac aagctcaacc gtatttctcg
    28801 ccagatgctg caagagatgg gccgtgaacc aacgccggaa gaactggctg aacgtatgct
    28861 gatgccggaa gacaagatcc gtaaagtgct gaagatcgcc aaagagccaa tctccatgga
    28921 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    28981 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    29041 ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa
    29101 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    29161 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgctgcg
    29221 tagcttcctg gacgattaat cggtaggccg gatcaggtgt tacgccgcac ccggcactag
    29281 tccctccgca caaacgccac cttttcggtg gcgtttttat cgcccacgca ccaccagcga
    29341 ctggtccagc tcgcgatacg cttcaaccag tttctccagt gaaacgcgac ttaaaccgct
    29401 gggatttggc agcacccaaa tctgcgtcga accaatggtg agagtttgtt tcccccactg
    29461 tgcaccgcgc tggctgaatc cctgttcata tgcttgtttg cccagaatcg ccaacgcctg
    29521 cggctgataa tcttctattt tttcaattag cttacgcccg cctgcgtgca gttcctgctt
    29581 tgacacttca ttggcttgca ccgttggacg gtctaccagt ttggtgacgc cacaacgata
    29641 atccagcaga tgctgtgcct cctgcggctt caactgacgg tcggtaaacc cggcctgata
    29701 tatcaccttc cagaagcggt ttgccggatg agcaaaggga aaacccgttc cggcggatga
    29761 aagaccaggg ttgataccgc aaaacacgac ccgtaaccct ggagccaaaa tatcctcaac
    29821 catatttact cctttctaac atcaccagga aaagtataac ggattgaaaa tacattgttt
    29881 ataaaaacag caggcgcgcg gtaatggctg gattgcgaca cggagttact ttataatccg
    29941 ctaccatggc cccttagctc agtggttaga gcaggcgact cataatcgct tggtcgctgg
    30001 ttcaagtcca gcaggggcca ccagacatag caaaggctga cgagaaatcg tcagcctttt
    30061 tctttttata tatcagttac tttgcgtgcc agtaagccgc cgcgcgtacc cgctgtgggt
    30121 catactgttc cgcttcaaag cgacggctta aattcttaac gactttgcct tcgccggtta
    30181 tccagatgaa gtaatcatcg gcagggattt gcatctgcgc cagacgcgca tctaccgcct
    30241 gctcatcatg tgccagccat tcgatattaa aaccatcaag gtgcgcgaga taatcctgac
    30301 aggcgttatc ccgcacgcta accagcgcac taacttgcgg tttaacggca agtttgctca
    30361 acgtttccag gcggcggcgc agtgcgggca ttccggattc atcgcagaca tacagctgat
    30421 acgcgtaatc ctctggcacc accagcgaac cgcgcggacc tgccaccgta agtttatcgc
    30481 ccggtttcgc ctgcatcgcc cagccgctgg cgaccccacc gtcgtgaata aagaaatcga
    30541 tcgccagttc atggcgtagt tcgtcataca gcggcgtata gtcacgcgac ggtgggcgtg
    30601 gtccttccgg ccagacgatg ccctcttccg ttaccgttgg cggcacaaag tgagcgtcag
    30661 gttgaggaaa gaagagtttg ctgtgatcgt caaagccgcg cgacgtaaaa ccgtccagcg
    30721 cctcgccgcc aaggacaatg cgctgaaaac cggcgctgat gcgctcaacg cgtagcacag
    30781 tcagttcacg gaagcgcaga tcattgcgaa cgcgctgtgg gtagcggggg gtgttattca
    30841 ttgttatcgc cttcgtgatg gtaatcagat atatctaaat aaattccaca aatgataatg
    30901 attgttaatc atgataaatg caagtgattt gtagcactga tatatctgta gtctgataag
    30961 actcgcaacc tcttatcaag catcattgct caatgctgga tgcggcgtga acggcttatc
    31021 cggcctacat gttgagtaaa cctgcgttta aaaatcattt ttgtacttgc agtaatgctt
    31081 atgtcagata taaattagat atatctaatt aagcaaaggg aggctgatat gagccatcat
    31141 cacgaagggt gctgtaaaca tgaaggccag ccacgccatg agggctgctg caaaggtgag
    31201 aagtcagaac acgagcactg cggacacggt caccagcatg aacacggtca atgctgcggt
    31261 ggtcgccacg gtcgcggcgg cggtcgtcga caacgtttct ttggtcacgg tgaattgcgt
    31321 ctggtgattc tggatattct ctcgcgcgat gacagccacg gttacgagtt gattaaagcg
    31381 attgaaaatc tgacccaggg gaattacacc ccaagcccgg gcgtcatcta cccgacgctg
    31441 gattttctgc aggagcagtc gctgattacc attcgcgaag aggaaggtgg taagaatcag
    31501 attgcgctga ccgaacaagg cgcgcagtgg ctggaagaaa accgcgaaca ggtagagatg
    31561 attgaagaac gcatcaaagc gcgttgcgtt ggcgctgcgc tgcgccagaa cccgcaaatg
    31621 aagcgggcgc tggataattt taaagcggtg ctggatttac gcgtcaacca gagcgatatc
    31681 actgatgcac aaataaaaaa gatcattgcg gtgatcgacc gcgccgcttt tgatattacc
    31741 caactggatt aatcaccgat tgccggatgc agcgtaccct acacaccccg taggcctgat
    31801 aagatgcgcc agcatcgcat caggcattgt gctccaaccg ccggatccgg cataccgatt
    31861 aatgcagcac cgtcaccgcg tcttccagtc ggctggcgcg gtgtttcacc atcgccgaca
    31921 cctgcgcact ctcttccact aactcggcgt ttttctgggt gatgagatta agctcatcca
    31981 ctacacgggt caggctggaa agcccatcgg cctgttccag cgttgaatgg ctaatctgag
    32041 ctatcaactg ggtgacgttt tttacctgcg ccacaatatc ttccatcgtc cgtccggcgg
    32101 cgtgtacctg ctgcgaacca gattgcacct tatcagcact ggcatcaatc agcttgcgaa
    32161 tatcgttggc agcattggcg ctgcggctgg ctaaatgacg cacttccccc gccaccactg
    32221 caaaaccttt gccctgttcg ccggcacgcg ccgcttccac cgccgcattc agggccagaa
    32281 tattggtctg aaacgcaata tcgttaatca gcgaagtaat ggtgccaatg cgctgggtac
    32341 tgtcggcaat atcgtccatt gtcttgatca ccgtagtcat cgcctccccg ccctgcactg
    32401 ccgcattact ggcggtgata gaaagtttat cggcagccga cgccgtggca gagttctgtt
    32461 tcaccgacgc cgccatttgg ttcatggtcg ccaccgtttg ctgaacgtta tcaactgtct
    32521 gctgggtatg ttcgttcagt tcatcggtgc ctttcgccag cgtctcactg ccgtttctga
    32581 cactggacac ctggcttgag acatcattaa ttagccaacg gcacatcagg ccaagctgcc
    32641 ctaccgcgcg taatgtcagc cccagctcat cgctgcgatt cagatgctca acactattac
    32701 gctctccggt cgccaccttc agtgcctgac gggcgacatt ttctatcggg cggacaatct
    32761 gccattcaaa acaggcgctt gccaacaata ccactaacgc acagaggata tacgtcacca
    32821 ccggggcagc aacaaaccaa agcatgaccg ccagcaagat aaacatcagg gtcatcacgc
    32881 cacgcgcccg ccagcgaagc ggtaatgaag gcagtttacc cagccagcct ttacgcacca
    32941 ccaggccttt atgaatacgc ttactggtac gtccggcgtt caacgctttg tacagcggct
    33001 ccacagccgc gatctcttca tccgtcgccc gggtacgaat cgacatatag ccactgattt
    33061 ttccctcgcg caccatcggt accgcattgg cccgcaccca ataatggtcg ccatttttgc
    33121 ggcgattttt cacgatgccg ctccagggct cccctttttt cagggtgaac cacatatccg
    33181 caaacgccgc ttttggcata tccgggtgac gcaccatgtt gtgcggctgc ccctgtaact
    33241 cttgcaaggt atagccgctc acctgcacaa aagtgtcatt agcatgagtg atatagcttt
    33301 gcagatcggt agtggacatc agagtggtat cgtccgccag cggggtattt tgctgggtga
    33361 catacggatg agaagacatg ttcgcgtcct gtgcaggtta tatggttgtt aacttcttgt
    33421 cagagtttat gtcggccccg ctgcagttat ctttaactga ttaatttgat ttagatcgca
    33481 atttgcgatt aaagcataaa tctaatccct tgatttaaaa tactttcact ctgttactat
    33541 acgaaaacgt taattatctt gcccaaaaat caggcaatta ttgccctgaa agcgtgcatt
    33601 tgcgcagcaa tcatcaaatc catacccgac aaaaaccgtg caaaataaca gcaaatgtta
    33661 gcaaatagca ttaaatattg cgcaaatgat aaccacattt gtgtttatcc cgattttcac
    33721 gatcgcagcc ggagtggcgc aatccctgca atacttaaat cggtatcatg tgatacgcga
    33781 gcctccggag catattttga acaggttacc ttcgagcgca tcggctttag cgtgcagcgc
    33841 ccacgccctg aatctcattg aaaagcgaac gttggatcat gaggagatga aagcacttaa
    33901 ccgggaggtg attgaatact tcaaagagca tgtcaatccg gggtttttag agtatcgcaa
    33961 atctgttacc gccggcgggg attacggagc cgtagagtgg caagcgggag gtttaaatac
    34021 gcttgtcgac acccagggac aggagtttat cgactgcctg ggaggttttg gaattttcaa
    34081 cgtggggcac cgtaatccag ttgtggtttc cgccgtacag aatcaacttg cgaaacaacc
    34141 gcttcacagc caggagctgc tcgatccgtt acgggcgatg ctggcgaaaa cccttgcggc
    34201 gctaacgccc ggtaaactga aatacagctt cttctgtaat agcggcaccg aatccgttga
    34261 agcggcgctg aagctggcga aggcttatca gtcaccgcgc ggcaagttta cttttattgc
    34321 caccagcggc gcgttccacg gtaaatcact tggcgcgctg tcggccacgg cgaaatcgac
    34381 cttccgcaaa ccgtttatgc cactgctgcc agggttccgt catgtaccgt ttggcaatat
    34441 cgaagccatg cgcacggctc ttaacgagtg caaaaaaacc ggtgatgatg ttgctgcggt
    34501 gatcctcgaa ccgattcagg gcgaaggtgg cgtaattctg ccgccgccag gctatctcac
    34561 cgccgtgcgt aagctgtgcg atgagttcgg cgcactgatg atcctcgatg aagtacaaac
    34621 gggcatgggg cgcacgggca agatgttcgc ctgcgagcat gagaacgtac agccggatat
    34681 cctttgtctg gcaaaagcgc tcggtggcgg cgtgatgccg attggcgcaa ccatcgccac
    34741 tgaagaggtg ttctcagtcc tgttcgacaa tccgttcctg cataccacca cctttggcgg
    34801 caacccgctg gcctgtgcgg cggcgctggc gaccatcaat gtgttgctgg agcagaactt
    34861 accggctcag gctgaacaaa aaggcgatat gttgctggac ggtttccgtc aactggcgcg
    34921 ggaatatccc gatctggtac aggaagcgcg tggtaaaggg atgttgatgg cgattgagtt
    34981 tgttgataac gaaatcggct ataactttgc cagcgagatg ttccgccagc gcgtactggt
    35041 ggccggaaca ctcaacaacg ctaaaacgat ccgcattgaa ccgccactga cactgaccat
    35101 tgaacagtgt gaactggtga tcaaagcggc gcgtaaggcg ctggcggcca tgcgagtcag
    35161 tgtcgaagaa gcgtaatatc aaatcggatg gcgatacgac gtcgccatcc gatcttttta
    35221 tacgatacgt acgcccgcag gcatcatccg ctccggcgtt aacagcacgc tttcactgcc
    35281 gtcatcggtt tccgcacaca acagcatgca ttccgacgtt tcaccgcgca ttttcgcttt
    35341 ttgcagattg cataagacca ccactgtttt ccccatcagc tcttcttcgc tgtagtacgg
    35401 caccaggctg gtcacggttt gcaacgtttt ttcacccaca tcaacctgta cgatgtacag
    35461 cttgtcggcg ttttcatggc gtttcacttc cacaatcttt ccgacacgca tttccagacg
    35521 tgcaaaatca gcgtaagcca cggtttccat tgtttcctcc cttcgagtaa aattttacta
    35581 aactatagca aaagtttttc tcgatcctgt aggctaaaaa tggagaatgc aggcgtgatc
    35641 acattcgcaa gccgctgtgt taccgttaca gcgtcaaaga aacgcgcttt atttactgaa
    35701 aacaggtgac ccgataagca cttcctctac aatgggggcg cacatcaggg aaagtaaaaa
    35761 aggtaaacat ggcaacacta aaagacatcg caatcgaagc tggcgtatcc ctggcgacag
    35821 tatccagggt cttgaatgac gatccgacat tgaatgtgaa agaagagacg aaacatcgca
    35881 ttctcgagat cgccgaaaag ctggagtaca agaccagtag cgcccgtaaa ctccagacag
    35941 gcgcagtcaa ccaacaccat attctggcta tctacagcta ccaacaggag ctggagatca
    36001 acgatcctta ctatctggcg atccgccacg gcattgaaac tcagtgcgaa aagctggcga
    36061 tcgaactgac caactgttat gaacacaacg gcttaccaga cattaaaaac gtcaccggta
    36121 ttttaattgt cggcaaaccc acgtccgccc tgcgcgccgc cgcctgtgcg ttgaccgaca
    36181 atatctgttt tatcgacttt cacgaacccg gcagcggtta cgatgcggtg gatatcgatc
    36241 tggcacgtat cagtaaagaa atcatcgatt tctatatcaa tcagggcgtt aatcgtattg
    36301 gttttattgg cggtgaagac ttacccggca aggcggatat tcgtgaggtc gcctttgcgg
    36361 aatatggtcg tctgaaacac gtggtacgcg aagaggatat ctggcgcggc ggtttttcca
    36421 gttcgtcggg ctatgaactg gcaaaacaga tgctggcgcg ggaagactat ccgaaggcac
    36481 tgtttgttgc ttccgattcc attgctatcg gcgtactgcg ggcaattcat gaacgtggcc
    36541 tgaacatccc acaggatatt tcgcttatca gcgttaacga tatccccacc gcgcgattta
    36601 cctttccgcc gctctccacc gtgcgcatcc attccgaaat gatgggaagt cagggcgtta
    36661 acctagtgta tgaaaaagcc cgcgatggtc gcgcgctgcc gctgttggtc ttcgttccca
    36721 gcaaattaaa actgcgcggc acgacccgtt aaatcccctt gcacgctgtc cggcaatcgt
    36781 ttttgccgga cagtgctgcc gtttattttc gtgatccagt taaagtaaat gcatttatcc
    36841 gctacttttt agtaaaattt tactaaactc cccagcaatt acacaaacta ccatcaccat
    36901 gaatggttcc gatttctctc taccgggagg ccctatgaat cgctgggaaa acattcagct
    36961 cacccacgaa aaccgacttg cgccgcgtgc gtactttttt tcatatgatt ctgttgcgca
    37021 ggctcgtact tttgcccgtg aaaccagcag cctgtttctg cccttaagcg gtcagtggaa
    37081 tttccacttt tttgaccatc cgctgcaagt gccagaagcc ttcacctctg agttaatggc
    37141 tgactggggg catattaccg tccccgccat gtggcaaatg gaaggtcacg gcaaattgca
    37201 atataccgac gaaggttttc cgttccccat cgatgtgccg tttgtcccca gcgataaccc
    37261 aaccggtgcc tatcaacgta ttttcaccct cagcgacggc tggcagggta aacagacgct
    37321 gattaaattt gacggcgtcg aaacctattt tgaagtctac gttaacggtc agtatgtggg
    37381 gttcagcaag ggcagtcgcc tgaccgcaga gtttgacatc agcgcgatgg ttaaaaccgg
    37441 cgacaacctg ttgtgtgtgc gcgtgatgca atgggcggac tctacctacg tggaagacca
    37501 ggatatgtgg tggtcggcgg ggattttccg cgatgtttat ctggtcggta aacaactcac
    37561 gcatattaac gatttcaccg ttcgtaccga ctttgacgaa gcctattgcg atgccacact
    37621 ttcctgcgaa gtggtgctgg aaaatctcgc cacctcccct gtcgtcacga cgctggaata
    37681 taccctgttc gacggcgaac gcgtggtgca cagcagcgcc attgatcatc tggcaattga
    37741 aaaactgacc agcgcccgct ttgcttttac tgtagaacag ccgcagcaat ggtcagcaga
    37801 atccccttat ctttaccatc tggtcatgac gctgaaagac gccgacggta acgttctgga
    37861 agtggtgcca caacgcgttg gcttccgtga tatcaaagtg cgcgacggtc tgttctggat
    37921 caataaccgt tatgtgatgc tgcacggcgt caaccgccac gacaacgatc atcgcaaagg
    37981 ccgcgccgtt gggatggatc gcgtcgagaa agatctccag ttgatgaagc agcacaacat
    38041 caactccgtg cgtaccgctc actacccgaa cgatccacgt ttttacgaac tgtgtgatat
    38101 ctacggcctg tttgtgatgg cggaaaccga cgtcgaatcg cacggctttg ctaacgtcgg
    38161 cgatatcagc cgcattaccg acgatccgca gtgggaaaac gtctacgtcg agcgcattgt
    38221 tcgccatatt cacgcgcaga aaaaccatcc gtcgatcatc atctggtcgc tgggcaatga
    38281 atccggctat ggctgtaaca tccgcgcgat gtaccacgcg gcgaaggcgc tggatgacac
    38341 gcgactggtg cattacgaag aagatcgcga tgctgaagtg gtcgatatta tttccaccat
    38401 gtacacccgc gtgccgctga tgaatgagtt tggtgaatac ccgcatccga agccgcgcat
    38461 catctgtgaa tatgctcatg cgatggggaa cggaccgggc gggctgacgg agtaccagaa
    38521 cgtcttctat aagcacgatt gcattcaggg acattatgtc tgggaatggt gcgaccacgg
    38581 gatccaggcg caggatgaca acggcaacgt ctggtataaa ttcggcggcg actacggcga
    38641 ctatcccaac aactataact tctgtcttga tggtttgatc tattccgatc agacgccggg
    38701 accaggcctg aaagagtaca aacaggttat tgcgccggta aaaatccacg cgctggatct
    38761 gactcgcggc gagttgaaag tcgaaaataa actatggttt accacgcttg atgactacac
    38821 cctgcacgca gaggtgcgcg ccgaaggtga aacgctcgcg acgcaacaga ttaaactgcg
    38881 cgacgttgcg ccgaacagcg aagccccctt acagatcacg ctgccgcagc tggacgcccg
    38941 cgaagcgttc ctcaacatta cggtgaccaa agattcccgc acccgctaca gcgaagccgg
    39001 gcattctatc gccacttatc agttcccgct gaaggaaaac accgcgcagc ctgtaccttt
    39061 cgcaccgaat aacgcccgtc cgctgacgct ggaagacgat cgtttgagct gtactgttcg
    39121 cggctacaac tttgcgatca ccttctcaaa aacgagtggt aaaccgacat cctggcaagt
    39181 gaatggcgag tcgctgctga cccgcgagcc aaagatcaac ttcttcaagc cgatgatcga
    39241 caaccataag caggagtacg aagggctgtg gcagccaaat catttgcaga tcatgcagga
    39301 gcatctgcgc gattttgtcg ttgagcaaag tgatgatgaa gtgttgatca tcagccgcac
    39361 ggttattgcc ccaccggtgt ttgatttcgg aatgcgctgc acctacatct ggcgcatcgc
    39421 tgccgatggc caggttaacg tggcgctttc cggcgagcgt tacggcgact atccgcacat
    39481 cattccgtgc atcggtttca ccatggggat taacggcgaa tacgatcagg tggcgtatta
    39541 cggtcgtgga ccgggcgaaa actacgccga cagccagcag gctaacatca tcgatatctg
    39601 gcgcagcacc gtcgatgcca tgttcgagaa ctatcccttc ccgcagaaca acggtaaccg
    39661 tcagcatgtc cgctggacgg cactgactaa ccgccacggc aacggtctgc tggtggttcc
    39721 gcagcgacca attaacttca gcgcctggca ctatacccag gaaaacatcc acgctgcaca
    39781 gcactgtaac gagctgcagc gcagtgatga catcactctg aatctcgacc accagctgct
    39841 tggcctcggc tccaactcct ggggcagcga ggtgctggac tcctggcgcg tctggttccg
    39901 tgacttcagc tacggcttta cgttgctgcc ggtttctggc ggagaagcta ccgcgcaaag
    39961 cctggcgtcg tatgagttcg gcgcagggtt cttttccacg aatttgcaca gcgagaataa
    40021 gcaatgagga tcatcgataa cttagaacag ttccgccaga tttacgcctc tggcaagaag
    40081 tggcaacgat gcgttgaagc gattgaaaat atcgacaaca ttcagcctgg cgtcgcccac
    40141 tccatcggtg actcattgac ttaccgcgtg gagacagact ccgcgaccga tgcgctattt
    40201 accgggcatc gacgctattt tgaagtgcat tactacctgc aagggcagca aaaaattgaa
    40261 tatgcgccga aagagacatt acaggtagtg gaatattatc gtgatgaaac tgaccgtgaa
    40321 tatttaaaag gctgcggaga aaccgtagag gtccacgaag ggcaaatcgt tatttgcgat
    40381 atccatgaag cgtatcggtt tatctgcaat aacgcggtca aaaaagtggt tctcaaagtc
    40441 accatcgaag atggttattt ccataacaaa taacaactac ggcggcaaaa ggagtttgcc
    40501 gccaccgcta ccctactcat tttcggagat gtgttatgtc tgataccaaa cgtaatacaa
    40561 tcggcaaatt cggcttgctc tcgctgactt ttgccgccgt ttacagcttt aacaacgtta
    40621 tcaataataa tattgagctt ggactggcct cggcaccgat gtttttcctc gcgacgattt
    40681 tttattttat tcccttctgt ctgatcattg cagaatttgt ttcgttaaat aaaaactcag
    40741 aagccggtgt ctacgcgtgg gtaaaaagtt cgctgggcgg acgttgggca tttattactg
    40801 cctataccta ctggttcgta aacctgttct ttttcacctc gctgttaccg cgcgttattg
    40861 cttatgcttc gtatgccttc ctcggctacg aatatattat gacgccggtt gccaccacca
    40921 ttatcagtat ggtgctgttc gccttctcca cctgggtttc taccaacggg gcgaaaatgc
    40981 tggggccaat tacctccgtc acttcaacgc tgatgctgct gttaacgctc tcctacattt
    41041 tgctggcagg aacggcactg gttggcggcg tacagcctgc tgaccccatc actgttgacg
    41101 cgatgatccc aaactttaac tgggcgttcc tcggcgttac cacctggatc tttatggccg
    41161 caggtggcgc ggagtccgtc gctgtgtacg ttaacgacgt caaaggcggt tcgaaatcgt
    41221 tcgttaaagt gatcatcctc gccgggattt ttatcggcgt actgtattcc gtctcctcgg
    41281 tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt tactggcgga tcggtacagg
    41341 tattccacgg catggcggcg tattttggtc taccggaagc actgatgaat cgctttgtcg
    41401 gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat gtggacggca acgccggtga
    41461 aaattttctt ctccgaaatc ccggaaggca tctttggtaa gaaaaccgtc gaactgaacg
    41521 aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct gatcgtcatc ccgctgatga
    41581 ttatcccgat gctcggttcc aataccgtgc aggatctgat gaatactatt attaatatga
    41641 ctgccgcagc gtccatgctt ccgccgttat tcatcatgct ggcttacctg aatttacgcg
    41701 ccaaattaga tcacctgcca cgcgatttcc gtatgggctc ccgccgcacc ggtattatcg
    41761 ttgtttcaat gctgattgcg atatttgccg taggatttgt cgcttcgaca ttcccgactg
    41821 gcgcgaatat tctgaccatc attttttata acgtcggcgg tattgttatc ttcctcggct
    41881 ttgcgtggtg gaaatacagt aaatatataa agggattaac ggctgaagag cgccatattg
    41941 aagcgacgcc agccagcaat gttgattaag cagaataata aaaaatgagc aggcattttc
    42001 cctcttcctg gatggggaga gggaaaatag tttctgcctt tatattttta taattacaac
    42061 gataaaaggc tgtacttttt ctttagctca tggattaaca caatgaaatt aatcactgca
    42121 ccatgcagag cattacttgc gctgccgttt tgctacgcct tttctgtgac cgctgatgaa
    42181 gcacgtccgg cagaacatga cgactcaaaa acccccgcaa tgacctcgac atcttctcct
    42241 tcatttcgtt tttacggcga attaggggtt ggtggatata tggatttaga gggtgagaat
    42301 aaacataaat acagcgacgg cacctatatt gaaggtggcc tggagatgaa gtacggctcc
    42361 tggttcggcc tgatttacgg cgaaggctgg accgtacagg ccgaccacga cggtaatgcc
    42421 tgggtgcctg accatagctg gggtggcttc gaaggtggga ttaaccgttt ctatggcggt
    42481 tatcgtacca atgatggcac cgaaatcatg ctcagtctgc gtcaggattc ctcgctggat
    42541 gacctgcaat ggtggggcga tttcacgccc gatctaggct acgtcattcc caatacccgc
    42601 gatattatga ctgcgctgaa ggtacagaac ttaagcggca actttcgtta tagcgttacc
    42661 gccacacctg ccggacatca tgacgaaagc aaagcctggc tacattttgg caagtacgat
    42721 cgctacgacg acaaatatac ctatccggca atgatgaacg gttacatcca gtatgacctt
    42781 acagaaggta tcacctggat gaatggtctg gaaatcaccg acggcacagg ccaactctat
    42841 ctcacgggcc tgcttacccc taactttgcc gctcgcgcct ggcaccatac cggacgcgcc
    42901 gacgggctgg acgtaccggg aagtgaaagc gggatgatgg tgagcgccat gtatgaagcg
    42961 ttaaagggcg tttatctctc caccgcttac acctacgcca aacatcgccc tgaccacgct
    43021 gacgatgaaa ccacctcttt catgcagttt ggtatctggt acgaatacgg cggcggacgt
    43081 ttcgcaacgg cttttgatag ccgcttctac atgaaaaatg cctctcacga tcccagcgac
    43141 caactcttcc tgatgcaata tttttactgg taataaggac agtaatctca tgaaaatcaa
    43201 aaccatttta acgccagtag cctgtgctct gctgatgagc ttttccgccc atgccgctaa
    43261 cgctgacaat tataaaaacg tgattaaccg tactggcgcg ccgcagtaca tgaaggatta
    43321 cgattacgac gatcaccagc gttttaatcc gtttttcgat ctcggagcct ggcatggtca
    43381 tctgttgcca gacggcccta acaccatggg cggttttcca ggcgttgcgc tgctgacgga
    43441 agaatacatc aactttatgg ccagcaattt cgatcgcctg accgtctggc aagacggcaa
    43501 gaaagtcgat ttcacgctgg aggcatacag tatacccggc gcgctggtgc aaaagttgat
    43561 atcgaaagat gtacaggtcg aaatgattct gcgcttcgcc acgccgcgca catcactact
    43621 ggaaaccaaa atcaccagcg ataaaccgct ggatctggtg tgggatggtg aactgctgga
    43681 aaaactggaa gcgaaagaag ggaaaccgct ttccgataaa accattgctg gcgagtaccc
    43741 tgactatcag cgcaaaatca gcgccacccg tgatggcctg aaagtcatct ttggcaaagt
    43801 gcgcgccacc tgggatctgc tgacctccgg tgaatcagaa tatcaggtgc ataaatccct
    43861 gccagtgcag actgaaatca acggcaatcg ctttaccagt aaggcgcata tcaacggttc
    43921 gacgacgctc tataccacct actcccatct gctgaccgct caggaagtta gcaaagagca
    43981 aatgcagatc cgcgatattc tggcgcgtcc ggcgttttat ctcactgcct cacagcaacg
    44041 ctgggaagag tatctgaaga aagggttaac caatccggat gcgacgccgg agcagacgcg
    44101 cgtcgcggtg aaagccatcg aaacgctcaa cggtaactgg cgatcgccgg gcggtgcggt
    44161 gaaatataac accgtcacgc cgtcggtgac cgggcgctgg ttctccggca atcagacctg
    44221 gccgtgggat acctggaagc aggcgtttgc gatggcgcat ttcaatccgg atatcgccaa
    44281 agagaatatc cgcgcggtct tctcctggca gatccagcca ggcgacagcg tgcgtccgca
    44341 ggatgtgggc tttgtccccg acctgatagc gtggaacctt agcccggagc gtggcggcga
    44401 tggcggcaac tggaacgaac gtaacaccaa acccagcctt gccgcctggt cggtgatgga
    44461 agtgtacaac gtcacccagg ataaaaactg gctggcagag atgtacccga aactggtggc
    44521 ctatcacgac tggtggctac gtaaccgcga tcacaacggc aacggcgtac cggaatatgg
    44581 cgcaacccgc gataaagccc acaatactga taatggcgag atgctgttta cagtgaaaaa
    44641 aggcaataaa gaagagacgc agtctgggct gaacaactac gcccgcgtgg tggagaaagg
    44701 ccagtacgac agtctggaaa ttccggcaca ggttgcggcg tcgtgggaat cggggcgtga
    44761 tgatgccgcc gtctttgggt ttatcgacaa agaacagttg gataaatatg tcgctaacgg
    44821 cggcaaacgt agcgactgga cggtgaaatt cgccgagaac cgcagtcagg acggaacgtt
    44881 gctgggctac tcgctattgc aggagtcggt ggatcaggcc agctatatgt acagcgataa
    44941 ccattatctg gcggagatgg cgacgattct cggtaagccg gaagaggcca aacgctatcg
    45001 ccagttggca caacagctcg cggactacat caacacttgt atgttcgacc cgactacaca
    45061 gttctactat gacgtgcgta ttgaagataa accgctggcg aacggctgtg cgggcaaacc
    45121 gattgtcgag cgcggtaaag ggccggaagg ctggtcaccg ctgtttaacg gtgcggcaac
    45181 gcaggccaac gccgacgcgg tggtgaaggt gatgctggat ccgaaagaat tcaacacctt
    45241 tgtcccgctg gggacggcag cgttaaccaa cccagccttt ggcgcagata tctactggcg
    45301 cgggcgcgta tgggtggatc agttctggtt tggtctgaaa gggatggagc gttacggtta
    45361 tcgcgatgac gccctgaaac tggctgatac gttcttccgg cacgccaaag gattaaccgc
    45421 cgatggccca attcaggaga attacaaccc gctcactggc gcgcagcaag gcgcaccaaa
    45481 tttctcctgg agtgccgcgc atttgtatat gttgtataac gatttttttc gtaagcagta
    45541 attagcaacg gctggcggat gcggcgtaaa cgccttatcc gccctacatg tgcaatcccc
    45601 ctggccggat aagacgcgtc aagcgtcgca tccggcgtta ccaccgggcg tattcttttt
    45661 gaatcccatc acaaaccccg cactcccctt ttcccttttc tccggcgacg gctaaattag
    45721 aactcatccg accacataac aattatttta catactggac aattttatga gctacccgtc
    45781 gctgttcgcc ccgctggatt taggttttac cacgttaaaa aaccgcgtgt tgatgggctc
    45841 aatgcacacc gggctggagg aatacccgga cggcgctgaa cggctggcag cgttttatgc
    45901 cgaacgcgcc cgtcacggcg tggcgttgat tgtcagcggc ggtatcgcac cagatttaac
    45961 aggcgttggc atggaaggcg gtgcaatgct caatgacgcc aaccagatcc cacaccatcg
    46021 caccattacc gaagccgtac atcaggaagg cggcaaaatc gccctgcaaa ttttgcatac
    46081 cgggcgctac agctaccaac cgcatctggt cgctccctcc gcattgcagg cccccatcaa
    46141 ccgtttcgtt cctcacgaac tcacccatga agagatcctg caactgatcg acgatttcgc
    46201 ccactgcgcg caattggcac gggaggcagg atacgacggt gtagaggtga tggggtccga
    46261 agggtatttg atcaacgaat ttctgacgct gcgcaccaat cagcgtagtg accagtgggg
    46321 cggcgattac cgcaaccgga tgcggtttgc cgtagaagta gtgggtgcgg tgcgcgaacg
    46381 tgtcggcaac gacttcatta ttatctaccg tttgtcgatg ctcgacctgg tcgaaggcgg
    46441 cggtactttt gccgaaacgg tagagctggc gcaggccatt gaagcggcgg gcgcaacgat
    46501 tatcaacacc ggcattggct ggcatgaagc acgtattccg accattgcca cacccgtgcc
    46561 gcgcggcgca tttagctggg tcacgcgcaa actgaaaggc cacgtttcgc tgccactggt
    46621 gaccaccaac cggattaacg atccgcaggt tgccgacgac attctctcgc gcggtgatgc
    46681 ggacatggta tcgatggcgc gaccgtttct cgctgatgcg gagctgctgt caaaagcgca
    46741 atcgggacga gccgatgaga tcaacacttg tattggctgc aaccaggcct gtctcgatca
    46801 gatcttcgtt ggcaaagtca cctcgtgcct ggtgaatcct cgcgcctgcc atgaaaccaa
    46861 aatgccaatc cttcctgccg tgcagaaaaa aaatctggcg gtggtcggtg cgggacctgc
    46921 tgggctggcg tttgccatta acgcggcggc gcgtgggcat caggtaacat tgtttgatgc
    46981 gcacagcgag atcggcgggc agtttaatat cgccaaacag atccccggca aagaggagtt
    47041 ttacgaaacg ctgcgctatt accgccggat gatcgaagtg acgggtgtga cgctaaaact
    47101 caatcacacc gtgacagcgg atcagttaca ggcgttcgat gaaacgatcc tcgccagtgg
    47161 gatcgtgccg cgcgttccgc ctatcgacgg gatcgatcat ccgaaggtat tgagttatct
    47221 cgatgtactg cgcgacaaag cgccggtggg caacaaagtt gccatcatcg gttgtggcgg
    47281 aattggtttt gatacggcga tgtatttaag tcagccggga gaatccacca gccagaatat
    47341 cgccgggttc tgtaatgagt ggggaatcga cagtagcctg caacaggccg gaggtttgag
    47401 tccgcaaggc atgcaaatcc cccgtagccc acggcagatt gtgatgctcc agcgcaaagc
    47461 cagcaaacca ggacaagggt taggtaaaac caccggctgg atccatcgca ccaccctgct
    47521 ctcacgcggc gtgaaaatga tcccaggcgt aagttaccag aagatcgacg atgacgggct
    47581 gcatgtagtg atcaacggcg aaacgcagat attagcagtg gacaatgtgg tgatctgcgc
    47641 agggcaagag ccaaaccgcg cattggcgca accgctgatt gatagtggga aaactgtgca
    47701 tttaattggc ggctgcgatg tagctatgga gctggacgca cggcgggcaa ttgcccaggg
    47761 aacacggctg gcgctggaga tttaaatcgt tgtttgaagt gccggatgcg acgcatccgg
    47821 cacgtcgcgt taatcaataa acaaggcggg agaaatgccg aatcgcgttg ccagtttttt
    47881 agcgtgttcc agcgttaatt tcctcttccc gctcaaaacg cgtgacacca tcgatttact
    47941 gccaatttcc ggaagatcgg aaagggttaa accatattga tccataaggg tacgaatcac
    48001 agctataccg ccaggcatgg cttgcgccat ggcattaaat tcagcaaatt cgggcgctga
    48061 ttcttcccac gcggttattt tggcacacac cagatccagc aaggggtttt caggatcgtt
    48121 gagcagcaga tgatctacca gttccagcgc ctgggtgtat tgttcctcgt tctgaatacc
    48181 cgccagaaaa ggtgccacag cagttaactt ttctcctgct tgcaagatgt cggcaatcgc
    48241 aatcattttt tccccttagt acgatgaacg gcggtaaaga aatcgtattc tttatgcgtc
    48301 ataacttcac gtatgtagca cttttgcgat tcaaaaaaga ccattgctac aacacgtaat
    48361 tcattgcctc cgacattgaa aacataatgc ttatccagat atttgaagtt atccagagaa
    48421 gggaatactg cttttaatga ctcaggtttt ttgaaatatc ccttagcaat cgtgttcccc
    48481 agagccacca actccgcttt atgttgcggg tatttttccg cagcatcttt caatgctttt
    48541 tgagttatca ggtgcattct tcatcacgtc cgttgacaaa ttggcaatat gataacatcc
    48601 gttgacagat tggcaacgaa tgaatttatc tgtgtgacgg aagaataaac gtagaaaaac
    48661 gctaaacaag agaatgtaga ggatggtgac agggattttt tgttttatgg aagcgcgatt
    48721 ccagaactgg gcagacattg ccggatgcga cgcttgccgc gtcttatccg acctgggggt
    48781 aatgtgctga acgtatgccg gataaggcgt ttacgccgca tccggcaggt aacccccaat
    48841 cttaccgacg acgccccagc ttcactgctt tcagcaccac aaatttatta ttcgtggcga
    48901 tggtggtgca gttgccgaaa attttcttca gtttatggaa gtaatccagg tgacggttgg
    48961 caacgatata cagctcgccg ttgattttca ggcagcggcg ggcatggtgg aacatctccc
    49021 aggcgacgtt atcggtcagc gcatgttgct gatgaaacgg cgggttgcag agcacagcat
    49081 taaaacggaa aggctccacg ccggagagcg cgttgttgat cataaactcg cagcgatcca
    49141 acgcctcggg catgttggtt tcaacgttca aacggctgga agcaaccgcc atcggtgatt
    49201 catcgacaaa caccactttc gcctgcgggt ttttatcgag caatgtcagg ccaataacgc
    49261 cattaccgca accgagatcg acaatctcac cttcgagatt ctctggcaga tgttgcataa
    49321 agaagcgcgc gccaatatcc agcccagtgc gggagaaaac attcgcatgg ttgtggatag
    49381 tccagtcagt gccttccagc ttccagctaa cggtctgcgg cgcatcggcc aacggcggtt
    49441 cgttgaaggt gcagttaatc aggcgcgctt tcttccatgc cagcgtggtg gtggtcggac
    49501 cgagcacttt ttcgaacaat tccagcgtgg aagtgtgaat gtcacgggct ttggcaccgg
    49561 caataatgcg cgtatctggc gtgaccactt tgcgcagcgc gcgcagttgt tgttccagca
    49621 atgccagtgt tttcggcact ttgatcagca ccacacccgg ctgttgcggg tagtcggcgg
    49681 tgctgtcgag gaacttcacg ctcgactcat caatgccgtt gagacgtaaa ttctcacgcg
    49741 tcgccaactc gctgatgtat gagtcgccaa tgctgtatgg cttatgttcc gccagcgcac
    49801 aacttaacgc accaaaggca tcattcagga tcaacaccgg gccgcggatt tctgtgtcgt
    49861 ccaactgttg cagcaaatat tcatccgccg cttcccacgc ctgtagtggg tttacgtcat
    49921 ccgtcgccgg aaaacgttgt agtgtcagtg aacggaaacc gttgtctaag tggctcatcg
    49981 gccctcctga atgataaaat ttcgacgttg cccctgaaaa gggtgcgtga gtataccctt
    50041 tttctctttt tcgtgggcgg ttatgagcaa tcttacttat cttcagggtt atcccgagca
    50101 gctactttcc caggtgcgaa cgctgataaa cgaacagcgt ctgggcgatg tgctggcaaa
    50161 acgctatccg ggaacacacg actacgccac cgataaagcc ctctggcaat atactcagga
    50221 tcttaaaaat cagtttctgc gcaatgcccc gccgatcact aaagtgatgt atgacaacaa
    50281 gatccatgtg ctgaaaaatg cgctcgggtt acataccgct gtttcgcgtg tgcagggcgg
    50341 caagctgaaa gcaaaggcgg agatccgcgt cgctaccgtg tttcgcaacg cgccggaacc
    50401 gtttttgcgc atgatcgtgg tgcacgagtt ggcgcacctg aaggagaaag agcataacaa
    50461 agcgttctat caattgtgct gccatatgga gccgcagtac caccagcttg agttcgacac
    50521 ccggctgtgg ctaacgcagt tgtcgcttgg gcaggacaaa atctgaaaat gatgtcaggc
    50581 gttatttttt cctcctttag cgcctaaaat cgacctcccc cctttcgtta aggataacga
    50641 ccatgctgcg cgtatttgcc caccttcttc gccgcgtttg tttctctcgc cggactctta
    50701 agattggctg tttattgttg cttatagcag gagcaaccct ttttattgcc gatcgcgtta
    50761 tggtcaacgc cagccaacaa ctgacctgga gcgatgtcaa cgccgttccg gcgcggaacg
    50821 tgggtttatt gctgggggcg aggccgggta atcgctactt tacccgacgt attgataccg
    50881 cagcagcgtt gtaccacgcc ggaaaagtga aatggctatt ggtgagcggc gataatggac
    50941 gtaaaaatta cgatgaagcg tcaggaatgc agcaagcctt gatcgctaaa ggcgtaccgg
    51001 caaaagtgat cttctgcgac tatgccggat tctcaacgct tgattcggtg gtgcgtgcca
    51061 acaaggtgtt tagtgaaaat catatcacca ttatctcgca agaatttcat aaccaacgcg
    51121 ccatctggct ggcaaaacag tatggtatcg atgctatcgg ttttaacgcg ccagacctga
    51181 atatgaagca cggtttatat actcaactgc gggaaaaact tgccagagtc agcgcggtga
    51241 ttgatgcgaa aatcctccat cgccagccga aatatctcgg gccgttggtt atgattgggc
    51301 cgtttagtga gcatggctgt ccggcaaaag aataatgcgt atctgcgcac gtcgaagata
    51361 aaaaaggcgt gctacagtga cgagagaatc cctttatgga gtatccacgc gttatgatac
    51421 gtttcgctgt gattggtacg aactggatca ctcgccagtt cgtcgaggcc gcccatgaga
    51481 gcggtaaata caagttaacc gccgtttatt cccgcagtct tgaacaggcc cagcacttcg
    51541 ccaatgattt ttctgtcgag catctgttta cgtcgctgga agcgatggca gaaagcgatg
    51601 ccattgacgc ggtgtatatt gccagcccga attccctgca cttttcccag acacaacttt
    51661 tccttagtca caaaattcat gtgatttgcg agaaaccgct ggcgtcgaat ctggcggaag
    51721 tggatgccgc cattgcctgt gcgcgggaaa atcaggtggt gctgtttgag gcatttaaaa
    51781 cggcctacct gccgaacttt catctgttac gccaggcgct gcctaaagtc ggcaaactgc
    51841 gtaaagtctt tttcaactat tgccagtatt cctcgcggta tcagcgttac ctggacggag
    51901 agaatcccaa cacctttaat ccgacattct ctaacggttc aattatggat atcggctttt
    51961 actgcctggc gtcggcggtg gcattattcg gtgaaccgaa aagcgtgcag gcaaccgcca
    52021 gtttgctggc aagcggtgtg gatgcccacg gcgtggtggt gatggattac ggtgatttca
    52081 gcgttacgtt gcagcactca aaagtgagcg attctgtcct ggcaagcgag attcagggcg
    52141 aagcgggatc gctggtgatt gaaaaactgt ctgaatgcca gaaagtctgc tttgtaccgc
    52201 gtggcagcca gatgcaggat ctcacccagc cacagcatat taataccatg ctctacgaag
    52261 cagagctgtt cgctaccctg gtggatgagc atctggtgaa tcatccaggg ctggcggtca
    52321 gtcgtatcac cgccaaactg ctgactgaga tccgccgcca gactggggtg atttttccgg
    52381 cagataacgt aaaactataa ttgccaaagt aacacagtgt aaaaggtatg taacagacca
    52441 ttgactgact gaatggtctg tcatactttg ttacctgcaa aggggagtaa cttcattgcc
    52501 ggtcgatcgt cattacgatg tgtgaaaaac cacatccggt caccgggcaa cccgaaagga
    52561 atacgcagac gtattccttt tttgttgtaa gtgagacctt gccggaaggc gaggtctatg
    52621 cataaaaagc agcggctgac gtcttccgac gttggccgtt tttttatgtg taaggaactt
    52681 ctatgaatac tgtcggcacg ccgttgctat ggggcggatt cgctgttgtt gtcaccatta
    52741 tgctggctat cgacctgttg ttgcaggggc gtcgtggggc acatgccatg accatgaaac
    52801 aggctgcggc ctggtcgctg gtctgggtga cgctgtcgtt actgtttaac gccgctttct
    52861 ggtggtatct ggtgcaaacc gaaggtcgcg ccgttgccga tccacaggca ctggcctttc
    52921 tcacgggtta tctgattgag aaatcgctgg cggtcgataa cgtctttgtc tggctgatgt
    52981 tgttcagtta tttctctgtt ccggcggcat tacaacgccg cgtgctggtg tatggtgtac
    53041 tcggagcgat tgttctgcgt accatcatga tcttcactgg tagctggctg atttcacagt
    53101 tcgactggat cttgtatatc ttcggtgcct tcctgctgtt taccggcgtg aagatggcgc
    53161 tggctcatga agatgaatca ggcattggcg acaagccgct ggtgcgctgg ctacgcggtc
    53221 atttgcgcat gaccgacacc atcgacaacg agcatttctt tgtgcgtaag aatggtttgt
    53281 tgtatgccac gccgctgatg ctggtgctga ttctggtgga gttgagcgac gtgattttcg
    53341 ccgtggatag tattccggct atcttcgccg tgaccaccga tccgtttatt gtgctgacct
    53401 caaacctgtt tgcgatcctc ggcctgcgtg cgatgtattt cctgctggca ggtgtggcag
    53461 agcgtttctc gatgctcaaa tatggtctgg cagtgattct ggtgtttatc ggtatcaaga
    53521 tgctgattgt cgacttctac catattccga tcgccgtctc gctgggcgtg gtgtttggca
    53581 ttctggtgat gacgtttatt atcaacgcct gggtgaatta tcggcatgat aagcagcggg
    53641 ttggatgatt tttaatctgc ctaagccgtg taccctgtca tcaacatgag caccgttttc
    53701 tccctctccc caaaaggggc gaggggactg accgagtact tttttgacgt tgtcatcagt
    53761 ctggaagccg cacgttgggc tttattttta tgtcaaataa atgtaaccat taagttgcaa
    53821 aatatgacct ctctttaaaa tccagcattt ttcgcttccc gaagctgtaa ctttccttat
    53881 actcgacctt gcaaacactt tgttacatcc tgaaagatgc gtcgacagaa cgcaccaggg
    53941 atgtgcgaca acacaatgaa aggatcgaaa aatgactacg caacgttcac cggggctatt
    54001 ccggcgtctg gctcatggca gcctggtaaa acaaatcctg gtcggcctta ttctggggat
    54061 tcttctggca tggatctcaa aacccgcggc ggaagctgtt ggtctgttag gtactttgtt
    54121 cgtcggcgca ctgaaagccg ttgcccccat cctggtgttg atgctggtaa tggcatctat
    54181 tgctaaccac cagcacgggc agaaaaccaa tatccgccct attttgttcc tctatctgct
    54241 gggcaccttc tctgctgctc tggccgcagt aatcttcagt tttgccttcc cttctaccct
    54301 gcacttgtcc agtagcgcgg gtgatatttc gccgccgtca ggcattgtag aagtgatgcg
    54361 cgggctggta atgagcatgg tttccaaccc cattgacgcg ctgctgaaag gtaactatat
    54421 cgggatcctg gtgtgggcaa ttggcctcgg tttcgcactg cgtcacggta acgaaaccac
    54481 caaaaacctg gtcaacgata tgtcgaatgc cgttaccttt atggtgaaac tggtgattca
    54541 cttcgcaccg atcggtattt ttggtctggt ttcttctacc ctggcaacca ccggtttctc
    54601 cacgctgtgg ggctacgcgc aactgctggt ggtactggtt ggctgtatgc tgctggtggc
    54661 gctggtggtt aacccactgc tggtatggtg gaaaattcgt cgtaacccgt tcccgctggt
    54721 gctgctgtgc ctgcgcgaaa gcggcgtgta tgccttcttc acccgcagct ctgcggcgaa
    54781 cattccggtg aatatggcgc tgtgtgaaaa gctgaatctg gatcgcgata cctattccgt
    54841 ttctattccg ctgggagcca ccatcaatat ggcaggcgca gcaatcacca ttaccgtgtt
    54901 gacgctagct gcggttaata cgctgggtat tccggttgat ctgcccacag cgctgctgtt
    54961 gagcgtagtg gcttctctgt gtgcctgtgg cgcatccggc gtggcggggg ggtctctgtt
    55021 gctgatccca ctggcctgta atatgttcgg tatttcgaac gatatcgcca tgcaggtggt
    55081 tgctgtcggc tttattatcg gcgtattgca ggactcctgt gaaaccgcgc tgaactcgtc
    55141 aactgacgtg ctgttcactg cggcaacttg ccaggcggaa gacgatcgtc tggcaaatag
    55201 cgccctgcgt aactaatact tagccccttt cgtctacggc ggaaggggtt ttctccactt
    55261 taaacggatc aattcccctt ctctgcatac gccagaaacg aatgatattc aggccattca
    55321 tcagcagaaa actgccttca atcatcgtgc cgcctatcga ccccgcccag aagttgtgaa
    55381 tcacccagca acacgttgaa aaccacatta cgcagcgcat ggtcagccct ttacagcgga
    55441 acagcgccca ggtactgaca atcgtgccga ttaccggcaa tagttcgaca ggatgatgga
    55501 acttcgcgag gccaattccg ccagtcagca caataaaaat cgccattacc cataagctgc
    55561 gcgtgcgtaa ggtaatcaat gtacgaatgg cattaaggat ggcactggca ccggcaggat
    55621 aggtgcccag aagaaaaaaa tgtacgccaa taacggcgct atagaccgaa agttgctttt
    55681 tgaagcgacg ttcgtcacga ttgaaaaatg ttgtgatacc aatcagaaag gcgatgacac
    55741 ccacgccctg ggccagccaa tacgcggtca tgataaatcc ttagcaggta tggaaaagca
    55801 aacggcgctt cacattatga aacgccgttt tttattaaca actcatttcg actttatagc
    55861 gttacgccgc ttttgaagat cgccagttcg cggaagtcgt tacgctcgtt acaggtttgc
    55921 ttaccgttgg caaactcaac gatagtgtcg ataaattctt ccagcaactg cggcatcgct
    55981 ttaccgtgga tcagctgacc cgcgtcaaag tcgatccagt gttttttctt cgccgccagt
    56041 tcgctgttgg tggcgatttt caccgtcggt acaaagccgc cgtacggcgt gccgcgacca
    56101 gtactgaaca gcaccatatg gcagcccgca cccgccaggg cgctggtcgc tacggcatcg
    56161 ttacccggcg cacttaacaa gttcagcccc ggcgttttca gacgctcgcc gtaacgcagc
    56221 acgtcaacca cgacactgga acccgctttc tgggtacagc caagcgattt atcttccagc
    56281 gtggtgatac cgcctgcttt gttccccggt gatgggtttt cgtagatcgg ctggtcatgg
    56341 gcaataaagt actgcttgaa gtcattgacc atggtgacca gtttttcaaa cgttgcttcg
    56401 tcgcgacaat ggtccatcag caactgctct gcgccaaaca tctccggcac ttcggtcagt
    56461 acggtagtac cgccgttagc aatcacgtag tcagagaaac gccccagcat cgggttagca
    56521 gtaataccag aaagaccgtc agaaccaccg cactccagac caaacttcag ttcgctgagt
    56581 ttgcccggct cgcgtttatc gttgcgcatc acgctataca gctgatgcaa atgcgcgatc
    56641 ccggcctcga tttcatcatc ctgctgttgg cagatcatga aatgaacgcg ttcaggatcg
    56701 atatcgccca acgtttcacg gaatgcggca acctggttat tttcgcaacc cagaccaatc
    56761 accagcactg cgcccgcgtt cgggtggcgc accatgtttt gcagcatggt gcgagtattg
    56821 atgtgatcgt cgcccagttg tgagcagccg taggtgtggc tgaagaggaa cacgccgtcg
    56881 gtaccttcag cgttgttggt ctctttcagg aaacgatttt ggatctgccg cgcgatgccg
    56941 ttgacacagc ccacggttgg caggatccac agctcattac gcaccccgac atcgccgtta
    57001 gcgcgacgat agatctgcac ttcacgatct gccgcttgcg caggcagatc ctgaaaatca
    57061 ggttgatagc gatactgatc cagatcgctc agattcgtgc gcgtattgtg ggcgtgaacg
    57121 tgttcccccg ccgcaatatc cgccaatgca taaccaatcg gcaggccata tttaatgacg
    57181 ttggcccctt ttgcgatatc cgttaacgca aatttatgtc cacgagcaac atcctggcgc
    57241 agcgtaacag tctggttatc gacactgact tctgtgcctt cagccaaatc cgctaaagca
    57301 accgcgacgt tatccagcgc atggatcttg atgtattgca tatcaacccc agaccttagt
    57361 tcagttcaat ggcgaagtag tcacgcgcat tgttaaagca aatatttttc accatctcgc
    57421 ccagcaggtt gatgtccgcc ggtgcttcgc ccgcttccac ccagcgaccg atcatctggc
    57481 acagaatgcg gcggaagtat tcgtgacggg tgtatgacag gaagctacgg ctgtcagtca
    57541 gcatgccgac aaagcggctc agcagaccga gctgcgccag ttgggtcatc tgacgttcca
    57601 taccgtcttt ctggtcgtta aaccaccagc cggaaccgaa ctgcattttg cccggcatac
    57661 cttcgccctg gaagttaccg atcatggtgc ccagcacttc gttatcgcgc gggttcaggc
    57721 agtagagaat ggttttcggc agcaggtttt cttcattctg cttgctcagc agcttagaca
    57781 gctcttccgc catcggacgg tcgttgatgg agtcaaagcc gacatccggc cccagcagtt
    57841 taaactgacg caggttgtta ttacgcagtg cgccaatgtg gtactgctgt acccagccgc
    57901 gacgtgcata ttcggcacca aggaacacca gcaccgcagt tttgaactgt gccacttcgt
    57961 gctcgctcag ggtttcgcct gccagacggc gcgcgaggat actgtccagt tccgcttcgt
    58021 tcgcttcagc aaacatcacc acatccagcg cgtggtcaga cactttacag ccgtgagcgg
    58081 cgaagtgatc caaacgttta gtcagggcag tttgcaggtc agcaaagcgg cgaatgtcgg
    58141 tatcggaaac ttcgcccagc ttcgccatgt agtcgttaaa ggtcgcctgt tcaatgttaa
    58201 aggctttgtc cggacgccag ctcggcagca ctttaatggt gaaagagccg tctttggcga
    58261 tctctgcgtg atgctccaga gaatcgatcg ggtcatcggt ggtgccgacc attttcacgt
    58321 tcatctgctg catgatgccg cgcgcggaga agttatcctg cgccagcagt tcgttacatt
    58381 cgttccagat ttcatcggca gttgacggag aaagcaattt gccagtgata ccaaacggac
    58441 gacgcagttc gaggtgcgtc cagtggtata acgggttgcc aatagtgtgc ggaacagtcg
    58501 ccgcccaggc gtcaaatttt tcacggtcag acgcatcacc ggtgcacaga cgctcggcca
    58561 caccgttggt acgcatagcg cgccatttgt agtgatcgcc cttcaaccag atgtcataca
    58621 ggtttttaaa acgatagtct tccgcaatct gctgcggcgg caaatggcaa tggtaatcga
    58681 aaatcggctg gtcttttgcg tagtcgtgat acagacggcg ggcaaattca gtatctaaca
    58741 ggaaatcttc agtcataaac ggagtcattt tcgtcttcct ctcaacgagt gagctagctt
    58801 gcttatggtg cgatgctgac aaagttatca caccaatttc cagagtccga agatattttc
    58861 gtgagttaga tcaataaacg tagttaaaaa aattactctc aaagtggtaa atctcgctgc
    58921 aggccgcgcc agcactggcc tcgccgtcgt caggtaatgt ccctacaaat attcgcacat
    58981 ttgtgatggc tctcaccttt taaagttgta tgacaagtta tctttctgcc gtcgcaaatc
    59041 ataagtcgac ggaatgcaaa ttgccgattc attcatttgt tagatgaatc gggttaaccg
    59101 gtacggaagc cgaattagca cgaaactttc atggcaacgt tcggggcgtg ccggtttttt
    59161 tcggttaccc ggtcgtaact aacatcttca gcctctggcg ggatgatggc cgcgcttcct
    59221 gcggatataa caaaacgatg aggttttaca tgcgtaaaat taaagggtta cgttggtata
    59281 tgatcgcact ggtgacgctc ggcaccgtgc ttggttacct gacgcgtaac actgtggcgg
    59341 cagctgcgcc aactctgatg gaagagttaa acatctccac ccaacagtat tcctatatca
    59401 tcgcagcgta ttctgctgct tatacggtca tgcaaccggt agcgggttat gtactggatg
    59461 tgctgggtac gaaaatcggt tatgcaatgt ttgctgtact gtgggccgtg ttctgtggtg
    59521 caactgcgct ggcaggtagt tggggtggcc tggcggttgc tcgtggtgcg gtcggtgccg
    59581 cggaagccgc gatgattccg gcgggtctga aagccagctc cgaatggttc ccggcgaaag
    59641 agcgttccat cgcggtaggt tactttaacg taggttcttc gattggtgcg atgattgcgc
    59701 cgccgctggt ggtatgggca atcgtgatgc acagctggca gatggcattt atcatctccg
    59761 gtgcgttgag ctttatctgg gcgatggcat ggctgatttt ctacaagcat ccgcgcgacc
    59821 agaaacatct gaccgatgaa gaacgcgact acattattaa tggtcaggaa gcccagcacc
    59881 aggttgacac agcgaagaaa atgtccgttg gtcagatcct gcgtaaccgt cagttctggg
    59941 gtatcgcgct gccgcgattc ctggcagaac cggcatgggg tacttttaac gcgtggatcc
    60001 cgctgttcat gtttaaagtt tacggcttta acctgaaaga gatcgcgatg tttgcctgga
    60061 tgccgatgct gtttgctgac ctcggttgta tcctcggtgg ttacctgccg ccgctgttcc
    60121 agcgttggtt tggtgtgaac ctgatcgttt cccgtaagat ggtcgtaacg ctgggcgctg
    60181 tgctgatgat tggcccgggt atgatcggtc tgttcaccaa cccgtatgtc gcgattatgc
    60241 tgctgtgtat cggtggtttt gctcaccagg cactgtctgg cgcactgatt acgctctctt
    60301 ctgacgtgtt cggtcgtaac gaagtggcga cggcgaacgg tttaaccggg atgtccgcat
    60361 ggctggcaag tacgctgttt gcactggtag tcggtgcact ggctgacacc atcggcttca
    60421 gcccgctgtt cgcagttctg gcagtgttcg acctgttagg tgcgctggtt atctggaccg
    60481 tgttgcagaa caaaccggcg attgaggtgg cgcaggaatc acataacgat cccgcaccgc
    60541 aacattaagg cttcgtgtaa gttttagttt cccttcaaag ccgccttctc aggcggcttt
    60601 ttcatcactg cgagtagagc taaactcgca aaagtggtat aacaaatata gtctgccgta
    60661 tcatgcctgg agcgcatatg gaaatcactg aaccacgccg tttgtatcaa caacttgccg
    60721 ctgacctgaa agagcgcatc gaacagggcg tctatctggt gggtgataaa ctgcctgccg
    60781 aacgctttat tgccgatgaa aaaaacgtca gccgcaccgt ggttcgtgaa gccatcatca
    60841 tgctggaagt tgaaggctat gtcgaagtgc gtaaaggttc aggtattcat gtggtttcca
    60901 accagccgcg ccatcagcag gcggctgaca ataatatgga attcgccaat tacggtccgt
    60961 ttgagttgct ccaggctcgc cagctcatcg aaagtaatat tgccgagttc gcggcaactc
    61021 aggtcacaaa acaggacatc atgaaattga tggccatcca ggagcaggca cgcggcgaac
    61081 aatgcttccg tgattccgag tgggatttgc agttccatat tcaggtagcc ctggcgacgc
    61141 agaactccgc cctggcggct atcgttgaaa aaatgtggac ccagcgtagt cataacccgt
    61201 actggaaaaa actgcacgaa cacattgatg cccgtaccgt cgataactgg tgtgatgacc
    61261 acgatcaaat cctcaaggcg ctgattcgca aagatcctca tgctgctaag ctggcaatgt
    61321 ggcagcacct ggaaaacacc aagatcatgt tatttaacga aaccagcgac gatttcgagt
    61381 tcaatgccga ccgctatctg ttcgccgaaa acccggtggt gcatctcgat acagccacca
    61441 gcggcagtaa atgaaattcc tgtccgacag gcgctacagt gcgcctgttt gtctcgtcgg
    61501 taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgcctccctc ccctgcattc
    61561 agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga cctttgttac
    61621 aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacga tttaacaggg
    61681 aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaagaca gcaataacat
    61741 tgcggtagtg catcatttaa aaccagcgtg gcgttaaccg attcaccagg aataatgaat
    61801 ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa ccctggccaa
    61861 tccgtcgatg attggcatgt tgtattttgt cttgtttgtc attttgttcc ttgaaaacgg
    61921 cttgcttccg gcggcctttt taccgggcga cagtttactg gtactggtcg gcgtattgat
    61981 tgcgaaaggc gcgatgggct atccgcaaac gattctgctg ctgaccgttg ccgccagcct
    62041 cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca ccgtacaaaa
    62101 ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt ttcataaaca
    62161 cggtttatcg gcgctgttaa ttggtcgctt tattgcgttt gtcagaacac tgctgccgac
    62221 gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact ggatgagtgg
    62281 tctgctgtgg gtgttgatcc tgacaactct gggttacatg ctcggcaaaa cgccggtatt
    62341 tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg tgctgctggt
    62401 gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa atcgggggta
    62461 agggatgcaa atacctcgca tgtcacttcg ccagctagcc tggtccggcg ctgttttact
    62521 tctggtcagc acactattgc tggcctggtc cgcggttcgc cagcaagagt ctacgctggc
    62581 gattcgtgcc gttcatcaag ggacaacgat gccagacggt ttttcaatct ggcatcacct
    62641 tgacgctcac ggcattcctt tcaaaagtat cacccccaaa aacgacactc tgttaattac
    62701 ctttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa cattgcccca
    62761 tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc tgacccggtt
    62821 gcgggataac tctcatcgct tcggataact tcctgttatc cgaaacagat cactcacttt
    62881 ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta ccgcttcgtt
    62941 gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc aagaatgaaa
    63001 taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta tgccactacc
    63061 ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga aaaacaccaa
    63121 aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc caactgttca
    63181 gatagccagc tgcgtgccga tcatcagaag aaaatcgcaa agcagaaaga tgaggtggcg
    63241 gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgcagataa gattgccaaa
    63301 cgcgaacgga aactggcaga agcgcaggaa gagctgaaaa agctgaaagc gcgcgactac
    63361 taactcacaa tagtcactac ttactcacct ggagaaaact atgtcgaaag aacacactac
    63421 ggaacatctg cgtgctgagt tgaaatccct ttccgatacg ctggaagagg tgcttagctc
    63481 atctggcgag aagtcaaaag aagagttgag taagattcgt agcaaagcgg agcaggcact
    63541 gaaacagagc cgttatcgcc tgggtgaaac cggtgatgcc attgccaaac aaacccgtgt
    63601 cgcggcggcg cgtgccgatg agtatgtgcg tgaaaatccg tggacgggcg tgggcattag
    63661 cgcagcaatc ggtgtagtgc tcggcgttct gctgtcgcgt cgttaattat ggcggacact
    63721 catcacgcgc aagggcccgg taaaagcgtt ctgggcatcg ggcagcgaat tgtttccatc
    63781 atggttgaaa tggtggagac acgtctgcgg ctggcggtgg tggagctgga agaggaaaaa
    63841 gcgaatctct ttcaactttt actgatgctg ggcctgacga tgcttttcgc tgcatttggt
    63901 cttatgagcc tgatggtgct agttatctgg gcggttgatc cgcaatatcg cctgaatgcg
    63961 atgattgcca ccaccgtggt gttgctgcta ctggcactga ttggcggcat ctggacgtta
    64021 cgtaaatcgc gtaagtctac gttgctgcgc cacacgcgcc atgagctggc aaacgatcgg
    64081 cagctgctcg aggaggagtc ccgtgagcag taaagtcgaa cgtgaacgac gtaaggcgca
    64141 actgcttagc cagatccagc aacaacggct ggatctttcc gccagtcgtc gtgaatggct
    64201 ggaggcaaca ggcgcttacg atcgtcgctg gaatatgctg ttgagtctgc gctcctgggc
    64261 gctggttggc agtagcgtga tggctatctg gacgattcgc catcctaata tgctggtccg
    64321 ctgggccaga cgcggttttg gtgtatggag cgcctggcgt ctggtgaaaa caaccctcaa
    64381 gcaacaacag cttcgcggtt aacttcctct ctggccggag aaattccggc cttatccctc
    64441 aaattttttg aaaatatttg acagttttcc ttgctaacaa tcacgcttcg tcacgtttat
    64501 gattctctcc atcgacagca acgacgctaa tagcgcgccc ttgcacaaaa aaacaatcag
    64561 cagcctgagc ggcgcagtgg agagtatgat gaaaaaatta gaagatgttg gtgtactggt
    64621 agcgcgtatt ttaatgccga ttctgtttat tacggcaggc tggggaaaaa ttactggcta
    64681 cgcgggtatc caacaatata tggaagcaat gggcgtcccc ggctttatgc tgccgctggt
    64741 gattctgctt gagtttggtg gtggtctggc aatcctgttc ggtttcctga cacgtaccac
    64801 agccctgttt actgcgggct ttacgctgct gacggcattt ttatttcaca gcaactttgc
    64861 agaaggcatg aactcgctga tgtttatgaa aaacctgact atttctggcg gattcctgct
    64921 gctggcaatt accggtccag gcgcattcag catcgaccgc ctgctgaata aaaagtggta
    64981 agcacgcgct atacttaacg ataaaaaacg agggggaagc tcctcgtttt tgctattgga
    65041 ggatagaaaa atgggtcaac tgattgacgg cgtctggcat gacacctggt acgataccaa
    65101 atccaccggc ggtaaatttc aacgttcagc ttccgcattt cgtaactggc tcactgccga
    65161 tggcgcgcca ggccccactg gcaaaggcgg ttttgccgca gagaaagagc gttatcatct
    65221 ctatgtttca ctcgcctgcc cgtgggcaca ccgcacgctg atcatgcgta agctcaaagg
    65281 actggaaccg tttatttccg tttccgtagt gaacccgctg atgctggaaa acggctggac
    65341 ctttgatgac agttttccgg gagcaaccgg cgacacactc tatcaacatg aatttctgta
    65401 tcagctttat ctccacgccg atccacacta cagcggacga gtgactgttc ccgtgctgtg
    65461 ggacaaaaaa aaccacacca tcgttagcaa cgaatcagca gaaatcatac gcatgttcaa
    65521 taccgcgttt gatgcgctgg gtgcgaaagc tggagattac tacccaccag ccctgcaacc
    65581 gaaaattgac gaacttaacg gctggattta cgacaccgtt aacaatggcg tgtataaagc
    65641 cggttttgcc accagccagc aagcctacga cgaggcggtg gcgaaagtgt ttgaatcgct
    65701 ggcgcgactg gaacagattt taggtcagca ccgttacctg accggcaacc agctaaccga
    65761 agccgatatt cgcctgtgga ccacgctggt gcgttttgat ccagtgtatg tgacccactt
    65821 caagtgtgat aagcaccgca tcagcgatta cctgaatctg tatggcttcc tgcgcgatat
    65881 ctaccagatg ccgggaattg ccgaaacagt caatttcgat cacatccgta atcattactt
    65941 ccgcagccat aagaccatca accctacggg gattatttca attggtccgt ggcaggatct
    66001 cgatgaaccg catggacgag atgttcgctt cggttaaaaa aaggcacctt acgaggtgcc
    66061 tctgataatt agctaagtac gtgttgttca tgtcggatgc ggcgtaaacg ccttatcaaa
    66121 cctacaaaac atttatattc aatatgttgc aatgaccgcg caggccagat aagcgtagcg
    66181 catctggcaa ttttgcgctt gcccttatcc ttcacagcgc cttattaatt catcgcgata
    66241 atatttactg ccatttcatt cacgtctatt cttaatttgc tgctttaaaa acaagtgatt
    66301 gagcaaaatt gaggcaaaaa tggactggta tctgaaagta ctaaaaaatt atgtcggttt
    66361 ccggggtcgt gcgcggcgca aagagtactg gatgtttatt ctggtcaaca tcatctttac
    66421 gttcgtgctg gggctactgg ataaaatgtt aggctggcaa cgtgccggtg gcgaaggcat
    66481 cctgacgaca atctacggta ttctggtgtt tttaccatgg tgggcagttc agttccgccg
    66541 cctgcacgac accgaccgct cggcgtggtg ggcactgcta ttcttaatcc cgttcatcgg
    66601 ctggcttatt atcatcgtct ttaactgcca ggcgggtacg ccaggcgaaa accgctttgg
    66661 accagatccg aaactggaac aagaataata attaagcacc ttgtaaaagg tgcttaatcg
    66721 attattttcc gctaaaaagt ttgggaattt cccgcagaca ccaggatttc gcctccccca
    66781 tactgtcacg acgccaggcc ataataatat cgatctcgct ggtcgattcc gggctgacga
    66841 cacgcaaccg cccttccgca atatcttttt cgaccatcgg atacggcatc gtcgccacgc
    66901 caagccccgc cagtaatgcc tgacgtttat cttcaatcgt gctcaccgtt aagcgcggct
    66961 gtttgtccag caactgtacg gtcaacaccg ggcgctcacg agcggtatcc gccaccgcaa
    67021 ttccacgata tttcacgcgc gtcacttcag ataacggttc cggctcctga tgaatcgggt
    67081 gatccggcgc ggcaacatag acgttcatta aggtatagag tttgcgcgag ttgatctccg
    67141 acgaggaacg aaaatgcata tccggcgcga taac
//
LOCUS       DAZGJT010000011        66907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZGJT010000011 DAZGJT010000000
VERSION     DAZGJT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 66907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.011"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..4479
                     /locus_tag="ACHKFK_000781"
     CDS             <1..4479
                     /locus_tag="ACHKFK_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001490035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9051195.1"
                     /translation="GMTVADADSIGIQIDGDKAVVNNDGDNAISNGGTGTQVNGDEAT
                     VNNNGSTTVDGKDSTGTEIAGNNATVTQEGELTVSSGGRGIDITGNNAKVDTKGKMTI
                     TGTDSVGVSINGDSATLTNTGDIDVSNSATGFSLVTNEGIISLAGSMKVGDFSTGMAL
                     SGDNNSVTLAAKDINVTGQKATGVNISGESNTVDITGNILVDKDQTADNAVVYFYDPS
                     VGVNISGNSNTVSLDGKLTVIADSELTSRKYMEFDGSQENISGLTVSGDGNTVNLNGG
                     IQFVGEKNALADGSTIADKRSYFGKTPLVSVDGQSKVYLNGDSTISGSLPLGYANILQ
                     LSNKAALEIGSDATFSMQDISVYEHYFTQTPQIIKVDTGSQVVNNGDVDIWNISFAGI
                     WGENSTGINNGNITLSQYDYSSPETSFSEPDHMAFLSSSGGSAVNNGTITAKVMEQHS
                     VLNLGSAAGVADPRVFNNSVSSMMGMEAYGKGTVLNSESGVIDMYGRGNIGMLAVDDS
                     AADNAGKITLDTLWVDQNDTTTLRTDLPSSTAIDYGVGMATGTNSGGGARSNGVATNQ
                     QGGVITVYNAGAAMAAYGASNMVINQGIINLEKNGNYDGGLGANMLVGMAVYNRGTAI
                     NDKTGVININVDTGQAFYNDGTGTILNYGEINLLGSPMDSADSHMGAIPENLDLLTAL
                     TGSGETDMRTASSGGFVTTKALANYGNETLNSNVAAKAWLYNQDKANLTINGELSIGQ
                     GLENSGLLDSDTISAAANVYNRASGSIITDQLSLTGSNSFFNEGNFSGSVAGSSYKQN
                     VVNTGTMAVMADGKSLISGSFLLYNEAGATLSNSSSAVSGGENAIVNVTRTGDSLAQV
                     NRGTITAVNGYSAIKTASTGSNSNGKWIWNTDTGVISGVNPNAPLIDLGRGYNFANAG
                     TINVQGDGAVAISGGTTSYTVQLVNSGTINVGTAQGKADGTNGTGLIGIKGNGSDTTI
                     NNAQSGVINVYADNSWAFGGKTKAIINNGEINLLCDTGCDIYAPGTTGTLNDHNSTTD
                     IIVPAATSTPTQGSVPTVPADSSAQQKLTNYTIGTNSDGTSGMLKANNLVISDNVKVN
                     TGYSAGTADTTVVINDVFKGENISGAENISSSTVMWNAQGSTDASGNVDVTMTKNAYT
                     DVVTDSSVNNVAQVLDSGYTNNDLYTSLNVGTTAELNSALKQISGSQATTVFNEARVL
                     SNRFSMLSDAAPEVANGLAFNVVAKGDPRAELGNNTQYDMMALRKSMTLTEYQNLSLE
                     YGIARLEGNGSDTVGDNGVTGGYSQFFGLKHQMAFDNGMSWNNALRYDVHNLDSSRSI
                     AYGDVNKTADANVKQQYLEFRSEGAKTFELREGLNVTPYAGVKLRHTLENGYQERNAG
                     DFNLSMNSGSETAVDSIVGLKLDYAGKEGWSANATLEGGPNLSYVKSQRTASISGAGS
                     QRFNIDDGQSGGGFNSLATMGVKYSSQESALQLDAFHWKEDGISDKGVMLNFKKTF"
     gene            4688..5549
                     /gene="pdxI"
                     /locus_tag="ACHKFK_000782"
                     /pseudo
     CDS             4688..5549
                     /gene="pdxI"
                     /locus_tag="ACHKFK_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310035.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
     gene            5848..6453
                     /gene="ynbA"
                     /locus_tag="ACHKFK_000783"
     CDS             5848..6453
                     /gene="ynbA"
                     /locus_tag="ACHKFK_000783"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9051196.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLVLVAQPILFLLLPIVLFIRMTLNALDGMLARECNQKTRLGAILNETGDVISDIVLY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            6453..7349
                     /gene="ynbB"
                     /locus_tag="ACHKFK_000784"
     CDS             6453..7349
                     /gene="ynbB"
                     /locus_tag="ACHKFK_000784"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9051197.1"
                     /translation="MLEKSLATLFALLILATLINRFLVWRLPERKGGEVTLRIRTWWG
                     IVICFSMVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWGIPLNYLLIGFNC
                     FELFLLFIPLAGFLILATWRVLVGDPSGFLHTVSAIFCGWVMTVFTLSHAAWLLMLPT
                     INIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQVLLAGLLIGISGFCGDVVMSATKRDVGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            7365..9122
                     /gene="ynbC"
                     /locus_tag="ACHKFK_000785"
     CDS             7365..9122
                     /gene="ynbC"
                     /locus_tag="ACHKFK_000785"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO9051198.1"
                     /translation="MENSRIPGGHFFTTSDNTALFYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVMVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPGLALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLSTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGV
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRFIDKIYLNSVGWRGIRQRKTHLQ
                     MLIKQAVAHLHAKGLAVRVVDIAAGHGRYVLDALANEPAISEILLRDYSEVNVAQGQE
                     MIAQRGMSGRARFEQGDAFNPAELSTLTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIDPGGILIYTGQPWHPQLELIAGVLTSHKDGKPWVMRVRSQGEMDSLVHDTGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            9136..10428
                     /gene="ynbD"
                     /locus_tag="ACHKFK_000786"
     CDS             9136..10428
                     /gene="ynbD"
                     /locus_tag="ACHKFK_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO9051199.1"
                     /translation="MLQGAGWLLLLAPFFFFTYGFLNQFTATQDLNNHDIPSQVFGWE
                     TAIPFLPWTIVPYWSLDLLYGFSLFICSSTFEQRRLVHRLILATVMACCGFFLYPLKF
                     SFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVRWRKVCGGW
                     FLLIAISTLTTWQHHFIDVITGLAVGMLIDWIVPVDRRWNYQKPDQRRIKIALPYVVG
                     ACSCIVLMELMIMLQLWWSVWLCWPVLSLFIIGRGYGGLGAITTGKDSQGKLPPAVYW
                     LTLPWRIGMWLSMRWFCLRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTFEFPRGRAT
                     KDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSALVVAAWLL
                     CYGHCKTVDEAISYIRARRSRIVLKEEHKAMLKLWENR"
     gene            complement(10482..11087)
                     /gene="azoR"
                     /locus_tag="ACHKFK_000787"
     CDS             complement(10482..11087)
                     /gene="azoR"
                     /locus_tag="ACHKFK_000787"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO9051200.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSTDEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAYDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAE"
     gene            11288..15190
                     /gene="hrpA"
                     /locus_tag="ACHKFK_000788"
     CDS             11288..15190
                     /gene="hrpA"
                     /locus_tag="ACHKFK_000788"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO9051201.1"
                     /translation="MTEQQKLTFTALQQRLDSMMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDHAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPADYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKTRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAIDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EVRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            15463..16263
                     /gene="ydcF"
                     /locus_tag="ACHKFK_000789"
     CDS             15463..16263
                     /gene="ydcF"
                     /locus_tag="ACHKFK_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO9051202.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLVQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRITGDNPDAPRWLSYPGFVPQLGNNADSVIFVNPLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIQAWQTLKHDAVLIEAMESRSLR"
     gene            16460..17899
                     /gene="aldA"
                     /locus_tag="ACHKFK_000790"
     CDS             16460..17899
                     /gene="aldA"
                     /locus_tag="ACHKFK_000790"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO9051203.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERATEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKSIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(17941..18942)
                     /gene="gap"
                     /locus_tag="ACHKFK_000791"
     CDS             complement(17941..18942)
                     /gene="gap"
                     /locus_tag="ACHKFK_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9051204.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSIDYTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDDNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKKATNNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            19131..19661
                     /gene="cybB"
                     /locus_tag="ACHKFK_000792"
     CDS             19131..19661
                     /gene="cybB"
                     /locus_tag="ACHKFK_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO9051205.1"
                     /translation="MGNKYSRLQISIHWLVFLLVIVAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPITPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            19906..20079
                     /locus_tag="ACHKFK_000793"
     CDS             19906..20079
                     /locus_tag="ACHKFK_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051206.1"
                     /translation="MKKLALILVMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSIRASKKTCTN"
     gene            20105..20173
                     /locus_tag="ACHKFK_000794"
     CDS             20105..20173
                     /locus_tag="ACHKFK_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051207.1"
                     /translation="MNPLVRCAGKIPALALTWTCRP"
     gene            complement(20151..20252)
                     /locus_tag="ACHKFK_000795"
                     /pseudo
     CDS             complement(20151..20252)
                     /locus_tag="ACHKFK_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            20263..20694
                     /locus_tag="ACHKFK_000796"
                     /pseudo
     CDS             20263..20694
                     /locus_tag="ACHKFK_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(20732..21655)
                     /gene="ydcI"
                     /locus_tag="ACHKFK_000797"
     CDS             complement(20732..21655)
                     /gene="ydcI"
                     /locus_tag="ACHKFK_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9051208.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMNGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            21872..23215
                     /gene="ydcJ"
                     /locus_tag="ACHKFK_000798"
     CDS             21872..23215
                     /gene="ydcJ"
                     /locus_tag="ACHKFK_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9051209.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQVHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKVLIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLGWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQACNHGDASREAFEQALGGPVLDEFQLYQEAEERSKRRCGLL"
     gene            23440..25095
                     /gene="opgD"
                     /locus_tag="ACHKFK_000799"
                     /gene_synonym="mdoD"
     CDS             23440..25095
                     /gene="opgD"
                     /locus_tag="ACHKFK_000799"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO9051210.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKNQVIMDVENHLYARKDIKQLGIAP
                     MTSMFNCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            25235..25459
                     /gene="ydcH"
                     /locus_tag="ACHKFK_000800"
     CDS             25235..25459
                     /gene="ydcH"
                     /locus_tag="ACHKFK_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO9051211.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQVSVKEV"
     gene            25522..26061
                     /gene="rimL"
                     /locus_tag="ACHKFK_000801"
     CDS             25522..26061
                     /gene="rimL"
                     /locus_tag="ACHKFK_000801"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO9051212.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDSQ"
     gene            complement(26053..27032)
                     /gene="ydcK"
                     /locus_tag="ACHKFK_000802"
                     /pseudo
     CDS             complement(26053..27032)
                     /gene="ydcK"
                     /locus_tag="ACHKFK_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
     gene            27156..28148
                     /gene="tehA"
                     /locus_tag="ACHKFK_000803"
     CDS             27156..28148
                     /gene="tehA"
                     /locus_tag="ACHKFK_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO9051213.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTGAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALD
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTTLRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            28145..28738
                     /gene="tehB"
                     /locus_tag="ACHKFK_000804"
     CDS             28145..28738
                     /gene="tehB"
                     /locus_tag="ACHKFK_000804"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO9051214.1"
                     /translation="MIIRDENYFTDKYELTHTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWEMVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            29041..29709
                     /gene="ydcL"
                     /locus_tag="ACHKFK_000805"
     CDS             29041..29709
                     /gene="ydcL"
                     /locus_tag="ACHKFK_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO9051215.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(29744..30934)
                     /gene="ydcO"
                     /locus_tag="ACHKFK_000806"
     CDS             complement(29744..30934)
                     /gene="ydcO"
                     /locus_tag="ACHKFK_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO9051216.1"
                     /translation="MTYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQAAIVAGA
                     TTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLTLNEAIGV
                     FIVTNVLIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQFTLCGSM
                     LLVWLATRAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYIPPDFSFAHSL
                     SVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVYSVGIAAI
                     TAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQMLAGLAL
                     LSTIGGSLYQALHNERERDAAVVAFLVTASGLTLFGIGSAFWGLIAGGVCYVVLNLIA
                     NRNR"
     gene            31008..31544
                     /gene="sutR"
                     /locus_tag="ACHKFK_000807"
     CDS             31008..31544
                     /gene="sutR"
                     /locus_tag="ACHKFK_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO9051217.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQRQAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVFVIDGQLDLCVDGEWQSLNCGEGVRFAADVTHVYR
                     NGGEQTVHFHSLIHYPRS"
     gene            31617..33578
                     /gene="rlhA"
                     /locus_tag="ACHKFK_000808"
     CDS             31617..33578
                     /gene="rlhA"
                     /locus_tag="ACHKFK_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO9051218.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLEQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTIYYARNVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDDLLKTLPKRKG"
     gene            complement(33670..33855)
                     /locus_tag="ACHKFK_000809"
                     /pseudo
     CDS             complement(33670..33855)
                     /locus_tag="ACHKFK_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2554 family protein"
     gene            complement(34107..34637)
                     /locus_tag="ACHKFK_000810"
     CDS             complement(34107..34637)
                     /locus_tag="ACHKFK_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051219.1"
                     /translation="MSQGKEPVIERIGMLTKRYPSRSAAARAWGININTLNSYFKGGQ
                     TQPAPRESILIRISESEGVSLEWLKEGKGELPNDIGIYGNFGCSGDKGDQLTQMLSFL
                     TKDEREQLTNLLARKGVETVLTLLNKENQDLLQLEGRKRLAALMIVDMPDDCVREILE
                     KTETSRQSNPVESKAG"
     gene            34828..35076
                     /locus_tag="ACHKFK_000811"
     CDS             34828..35076
                     /locus_tag="ACHKFK_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9051220.1"
                     /translation="MSRREVSDHDWPPEIIKARLHMAGLSLRSLSLKAGYSRDSLKSV
                     LRTPCRPYQQIIADALGVSPEEIWPSRYQVKSYMRKAS"
     gene            35078..37168
                     /locus_tag="ACHKFK_000812"
     CDS             35078..37168
                     /locus_tag="ACHKFK_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9051221.1"
                     /translation="MYVIAKELIGAPGMPATTKGIRQALQRYVQGKSCCSRRRSGSKA
                     TEYSIDCLPEVTQQALRERYALQLMTQKADESPAPVVTKARRSPAVVDAVEAYRGSPQ
                     LMVERLNALTENQRKVAEARIAIVSEVLKIAQQPGFSCAKAIRFLVNSLARSQLDERI
                     VAMVETANAKKGNSRALSEITLKRWIAAFNKAQNAAERLLLLAPGKRQEIKAEDINWL
                     PEFLAQYRQSNGRPMTEAYEDFVAEWQHRHADEPYMLDIMPSYDTIRRAMKKLPEVVK
                     QKGRVTGSEYRQLEGFTRRDWSRMPVNYVWIGDGHGMKLKCAHPVHGRPFAPEVTFVI
                     DGGTRFVVGWSLDLAENVFAVAGAIQHGIRHHGKPFLYYSDNGSGETADVLDKEIVGI
                     LPRLGINHPTGIAGNPQGRGIIERLNRTLPMRIARKYRTYFGKGADRETLRKTNRDLR
                     SAFSALQQGKRLNARQQSAMRDLPSWSELIDAIRDGVEWYNNRPHDELPMKPNGKHYS
                     PAEFRKKRLAEEDTEIEWLSDVELRDMFRPMVERPVRRCEIRWLNNIYYAPELRDEHG
                     RKVLISYDIHDAERITVRRPDGSVICEAVWDGNKREAFPVSAEYYKQQQRLKGMRKRA
                     EEKIRDAEDEVVNVLEHKPQEPWLENIYRPVGNTVTVQQPVADDEPDEEYERNFQRGL
                     QLLEAKLKESDPLA"
     gene            37251..38183
                     /locus_tag="ACHKFK_000813"
     CDS             37251..38183
                     /locus_tag="ACHKFK_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9051222.1"
                     /translation="MTDINDVIKTIDELIDGGVLTQYAIAREAGISDGTLSAFRKGKY
                     KGDNAAVAASLRSWYENWNKQSALPEPPQFVETQTVQELRALFQAVRLMGCINVIVGV
                     PGVGKTATARNYCQEQPNTWMITLSPAHSSVTECLLELADALGIDYTRANKGALSRAI
                     RRRLMGTRGLVIVDEADHLGIDGLEQLRAIQDATGIGMVLIGNPRGLFKGGRRAFDDL
                     SRLFSRLARTKQLRKAKKADVLAIAMAWGISGEAELAVMQAIAEKPGALRVLTHTLNQ
                     AWLTASGEGAALTEKHINAAFKEVYTNPELLSQV"
     gene            38186..38407
                     /locus_tag="ACHKFK_000814"
     CDS             38186..38407
                     /locus_tag="ACHKFK_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051223.1"
                     /translation="MAVFNIPDIYGRFYLVNFDNVKVISLAENKECGDLLFEFNDRTR
                     MVISAGLDREGATEVYSGICRSVGAKQVS"
     gene            38420..38674
                     /locus_tag="ACHKFK_000815"
     CDS             38420..38674
                     /locus_tag="ACHKFK_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051224.1"
                     /translation="MNMQSCGNKMNLFDSLNSARRLTELAGAVLERSKRYPQRFALKT
                     TPPVGNVQGTGEIEITIQTNGLRRRVKATRISGCTVYWEV"
     gene            38676..38957
                     /locus_tag="ACHKFK_000816"
     CDS             38676..38957
                     /locus_tag="ACHKFK_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor protein"
                     /protein_id="HFO9051225.1"
                     /translation="MKKNLIAWAWSSGLIEFGYVLPEGALPIVAGKPATVRHVIEVMA
                     RHGRDEQEQLLVPGIPEAVTEEEAFNAMIRFCREVRRRVSYPNRTRTRG"
     gene            38954..39226
                     /locus_tag="ACHKFK_000817"
     CDS             38954..39226
                     /locus_tag="ACHKFK_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051226.1"
                     /translation="MSKVVRIIFEYKEDVICKNSDGSTHRGVSLDIRSTGIKKKDNGP
                     AMIFGVVMLAESRNFAEFVAMKASAFMKDRGMASGVINGNEFNQQG"
     gene            39231..39524
                     /locus_tag="ACHKFK_000818"
     CDS             39231..39524
                     /locus_tag="ACHKFK_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051227.1"
                     /translation="MSKVRVIFEFNHVSHDEKLAGNDCVEVHEKIGVDVKTERDTDNR
                     PTSLCDVYASILQYHSPAIIQFLSAEFQASAQSFGADAIIKRHRVHKASGTLQ"
     gene            39536..40066
                     /locus_tag="ACHKFK_000819"
     CDS             39536..40066
                     /locus_tag="ACHKFK_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9051228.1"
                     /translation="MAKRVTKLKAAAEAAPQTREEVSRDIRTLGDIQREALRLETAMN
                     DEVAEITARYTPQIENLKKQIKVLFKGIQDWCKTNRDELTNGGKTKTANLTTGTVSWR
                     LGTPSCSVSRDVEGVIEMLRRMGLERFIRTKEEVNKEAVLAEPDAVKGIAGIKVNKGA
                     ESFYVEPFEQDAGLNK"
     gene            40212..40745
                     /locus_tag="ACHKFK_000820"
     CDS             40212..40745
                     /locus_tag="ACHKFK_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051229.1"
                     /translation="MIDAKVLEGVKNWLCIYGRLTCGVLAEKMNMPPSSMVYFLRDAV
                     DAGVLTECNGFYDIPRPRPVQPVRRKCSQESAADDVQWCGFRKSLPWIEGHDIPSMAW
                     EFAQGVLTCETVYVVAEVDEQAMKEGVPQFVMAYIDIRLGVIICGLSGWNITEHVLRY
                     LIVDRTAAPAGISAEVA"
     gene            40745..41260
                     /locus_tag="ACHKFK_000821"
     CDS             40745..41260
                     /locus_tag="ACHKFK_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051230.1"
                     /translation="MFFKTSNPSALAAWQKYQQDCQKVKDEAKRLEAVLNVACRSVFV
                     SGISGFYFKGLRFTEDKYPFHRDLWRKPTASNGWSCTPRTSRIPKTLRVASDELNSLW
                     REYSPVTYARTDALLFWLGIDFSAILFGPVKWFCVDDVIYLQCGVKPAKQKMTEILSD
                     EFYAAEKRVRG"
     gene            41264..41815
                     /locus_tag="ACHKFK_000822"
     CDS             41264..41815
                     /locus_tag="ACHKFK_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SANT/Myb-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFO9051231.1"
                     /translation="MMKLQPMGLKGRAPAHVRPWTPEEDALLIALYSSTPVKDIAARI
                     KRTVWAVYNRTGVLRSSYPELLKYKHPRFTPDEDKFIRKNARTMTCQQMGEYLGRNKD
                     SVRCRAGMIGAGLTKCGELRPGTRISDDDVRLIRALRDSDYPRRLSFREIGEKFGISE
                     HSAHAVYYRRRTAEDAVLRELTP"
     gene            41812..42144
                     /locus_tag="ACHKFK_000823"
     CDS             41812..42144
                     /locus_tag="ACHKFK_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051232.1"
                     /translation="MITTLFVESDEPLVCAAGMPLCGGTLTGVYFGDLRGYPWHSLDD
                     AFPPDMEAVVLIVQYGDQQELRIGHMGYEGFFIDEETGACLEDEDGQVTHWCHLSALP
                     ELQGIHNG"
     gene            42282..42569
                     /locus_tag="ACHKFK_000824"
     CDS             42282..42569
                     /locus_tag="ACHKFK_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051233.1"
                     /translation="MEKEIKFAPKSIDEELAKIGMLERMRDIIEYAIKENLAAREALL
                     IMEREINLIKDAVSLDNKIAREEYVRRRLGVDGSAILTSEHYAKIFNLFQR"
     gene            complement(42550..42789)
                     /locus_tag="ACHKFK_000825"
     CDS             complement(42550..42789)
                     /locus_tag="ACHKFK_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051234.1"
                     /translation="MSDIKNALDVAAFTMAGNLMVTLFDKGLISREEAIHIINLAQKN
                     STPENAEDIQDGSYINTDWHFDNLRACMGITAEKD"
     gene            42860..43372
                     /locus_tag="ACHKFK_000826"
     CDS             42860..43372
                     /locus_tag="ACHKFK_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFO9051235.1"
                     /translation="MNRASLITLIHVAKRDLQLDHDTYTSALLAATGKSSCRDMSPGE
                     LSRVLDVFKKRGFKVRQKPVNRALKPGTVTAKIRAIWKVMHRQGFISDGGETALNRWV
                     KSQTAAQNDGEGVANWQWLEQQPALASDVLERLKRWHRRKMLAALDMSERAVMSYERV
                     CRLYEKSFPR"
     gene            43443..43865
                     /locus_tag="ACHKFK_000827"
     CDS             43443..43865
                     /locus_tag="ACHKFK_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFO9051236.1"
                     /translation="MAETQMSMFGDSEQLHALIDRLDDIPDDELKKNWPGTLRDLVDV
                     ISAELHRQGIEPAQAGMLARKVAAAQAGYMGGRGYYLPVGESLFTELRNNEIFFRWSQ
                     GEKIETLRRHYQMSETQIYSVIRNQRRLHQERTQPRLF"
     gene            43937..44437
                     /locus_tag="ACHKFK_000828"
     CDS             43937..44437
                     /locus_tag="ACHKFK_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9051237.1"
                     /translation="MPKLPAPLRKKLIALVLAGAGTFTIATHYTGYWEGKENTTYIDP
                     TGTPTICYGHTGPDVKPGMTKTDEECLELLEKDMKWAFAAIDRHVQVPLTRGQTVALA
                     SWIFWAGETNFRNSTLLRLINAGQMPASCKQYIRWIYSKGVKLPGLEARRSADEWLCR
                     YDLPKV"
     gene            44550..44900
                     /locus_tag="ACHKFK_000829"
     CDS             44550..44900
                     /locus_tag="ACHKFK_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051238.1"
                     /translation="MAALGVMSVLAAVVWGMHQKTQRLEDNNHVLVRERDEARLVLAN
                     QQRTLQLISQISKAATNEKQQNMQHSEEQQSIVRRSLATVPAAAVPVPDDVADRVRRA
                     VCEIRPCAAGADPR"
     gene            44884..45102
                     /locus_tag="ACHKFK_000830"
     CDS             44884..45102
                     /locus_tag="ACHKFK_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051239.1"
                     /translation="MPIPAEWLADCLVPPAPQPFTFGASVTYNLQLLAVIKNCNVDKA
                     SIRRLETQRQHEFTDMAGTTAVPAGKTK"
     gene            45113..45340
                     /locus_tag="ACHKFK_000831"
     CDS             45113..45340
                     /locus_tag="ACHKFK_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9051240.1"
                     /translation="MDDSDCAQAVMERATERALCDRLTRKRRVAETPGRRVCADCGGD
                     IPAARLVAVPDAIRCVNCQNIMEAGHVGQHR"
     gene            45321..45632
                     /locus_tag="ACHKFK_000832"
     CDS             45321..45632
                     /locus_tag="ACHKFK_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2730 family protein"
                     /protein_id="HFO9051241.1"
                     /translation="MWVSIVKDYVVPILSATATAGGIFMALMRKTFVPREAFEKLSDR
                     VERVESRISGLPTQQEVNRLNIEITTLRGELKATNATLKSVSHQNELLLEQAVRKKHN
                     E"
     gene            45625..45915
                     /locus_tag="ACHKFK_000833"
     CDS             45625..45915
                     /locus_tag="ACHKFK_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HFO9051242.1"
                     /translation="MSDFITEDQRLVILRSLADYNGELGESVLQDCLDDYGHRVSRDT
                     VHTHIAWLAEQGLVRKRTLVNGYFIAELTGRGQDVAEGRATVPGVKKPRARG"
     gene            45918..46499
                     /locus_tag="ACHKFK_000834"
     CDS             45918..46499
                     /locus_tag="ACHKFK_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp27 family protein"
                     /protein_id="HFO9051243.1"
                     /translation="MDKPTRGRVRKVDLLPDSIRKPLLEMLREKRLTQVQIREEINRL
                     IREAGLPEEQQLSPAAISREASRNELIARNLRDLREQTKAMMAELGDKPTGETTALIL
                     EMSRALMYRRLRAATESLNSDSDVDMRLIKDILLSAQRAESAAERSIKREKEIRAAFA
                     EEMANAVTDELRGVDGMSEQIEFRIRQMLLGKA"
     gene            46510..46758
                     /locus_tag="ACHKFK_000835"
     CDS             46510..46758
                     /locus_tag="ACHKFK_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051244.1"
                     /translation="MVTDNRSLMSSAHQKAFSLATQLPPETIKACRELTELSIAGEIS
                     LEQLHSLLAGAVTDELSGVDVMTEELEFRIKSILTGKA"
     gene            46758..48422
                     /locus_tag="ACHKFK_000836"
     CDS             46758..48422
                     /locus_tag="ACHKFK_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051245.1"
                     /translation="MNKAKEKSPSLIQMTPPRKIDLSGEKALLGVDVPDSLDLPGDMP
                     VFLDYQARWFADESQVCIAEKSRRTGLTWAEAGRNVITAAKPKRRGGRNVFYVGSKQE
                     MALEYISACALFSRAFNQLADADVYEQTFWDRDKKEEILTYMIRFPNSGFKIQALSSR
                     PSNLRGLQGDVVIDEAAFHESLDELLKAAVALTMWGARVRIISTHNGVDNLFNQYIQE
                     AREGRKDYSVHRITLDDAIADGLYRRICYVTDQEWSPESEQKWRDDLYKNAPTREDAD
                     EEYGCIPKKSGGAYIPHALIEMAMIRDIPILTFEAPDDFISRAAWLRESEVLTWCEEH
                     LKPLTEKLNPRSRFSFGEDFARTGDLSCFVLLEITELLAKREVFRVELRNLPYAQQEQ
                     VMMYILTRVPALVGAAFDATGNGGYLAEAALLAFGPDIIDCVMLSPKWYGEWMPKLKA
                     EFEDQNILVARHQTTLDDLRHVKVVNGIPQIDKGRTKDQNATAANARRHGDFAVALCM
                     ANRASYMEGFILDESACQALPERSRAMEGGYRDDDEAYHEFDRGCW"
     gene            48422..50011
                     /locus_tag="ACHKFK_000837"
     CDS             48422..50011
                     /locus_tag="ACHKFK_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFO9051246.1"
                     /translation="MGRIIDLDGKPFSFDPEMQSTALDIPQIASRYVEHPASGITPNR
                     AAQCLRGAERGDLIAQSDLAADIEEKDTHLFAELGKRRLAIQGVPWSIEPPLNASANE
                     KKDAEMLDEYLHSADWFDAMLFDATDAILKGYSCMEIEHGMLGKMHIIRAIRWRDSGH
                     FCLNPDDLSELRLRDGSHAGVAFQPFGWIVHQSRSRTGYGGATGLVRTLIWPFIFKNY
                     SVRDLAEFLEVYGLPMKVGKYPSGATPEQKSALMRAVMDIGRRTGGIIPAGMSLEFQA
                     AANGQADPFETMISWGERSISKAILGGTLTTEAGDKGARSLGEVHNEVRREIRDSDLR
                     QLAATLNRDLVYPLYALNTAHAIDIRRLPRICFQTKEPGDITKIASAVMQLSAGMDIP
                     DPWVRDQTGIPQPTPGEAIFRVRQSSNEPAMPDRETLPEKQEKTEQTALSARLPEAKN
                     TPRDELDDMGDAVPARRLQEAIDPVLEPVIDVIRTRGLAEALADLPALYREMDDSRLM
                     TLLSDAMFAAEMKGMLDGSGD"
     gene            49995..51326
                     /locus_tag="ACHKFK_000838"
     CDS             49995..51326
                     /locus_tag="ACHKFK_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFO9051247.1"
                     /translation="MAQGIDLGYAATLPSKETVAYFRAKGAHISWNWFETDADVHARS
                     FTAAKTARLDVVTTLQAEVQRAIDEGISQKEFIRTLTPRLQKLGWWGKQIVVDSDGNA
                     EEVQQGSPRRLALIYNVNTRVAYNAGRYTQMMNNTDTHPFWQYVAVMDSRTRPSHSAL
                     NGLVFRYDDPFWKTHYPPNGWNCRCRVRPLSQARLDAMGLSVSSGEDHLSTRNVEAGV
                     DKQTGEVREMPVTTYSDGARTMTPDVGWSYNPGSAAFGTDQALIRKLIEVKSPALREM
                     VVQEMNNSPERQLAFRIWAKNIMQTRRGGNDIRTLGFMTESIAQAVESRTGTPPARLL
                     AMSGKNVLHADSVKHQNDGIALTPEDFGRLPAMLARPKAVLWDKRHNNLMYIVENQDG
                     SVQIAVNAPYSLKRQPDKLDVIVNAYRVINMDKLKSDIRGGMLEVLEGQID"
     gene            complement(51328..51407)
                     /locus_tag="ACHKFK_000839"
     tRNA            complement(51328..51407)
                     /locus_tag="ACHKFK_000839"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            51448..51921
                     /locus_tag="ACHKFK_000840"
     CDS             51448..51921
                     /locus_tag="ACHKFK_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO9051248.1"
                     /translation="MSSIDAAVVVDVTRLQRVFARLQFVGGGKDLARSVAASLLSSSE
                     MAFEQEKEPDGERWHDWSDPYREWRTRKGYTPGKILTLNGDLARRLTTDYGDTWALIG
                     SNEPYAAIHQWGGLPGMPPGPAAIGARPYMGFDQVAEQEIMDEIRKRFKKATETP"
     gene            52099..53232
                     /locus_tag="ACHKFK_000841"
     CDS             52099..53232
                     /locus_tag="ACHKFK_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFO9051249.1"
                     /translation="MKTENHPKDANRPSGLAVLSASLTASGDGWCQLLPAGRVKARDG
                     RPEKPTEGWLINESACNRMKAGLSALHQPLLIDYDHHSVNAQKNGFKAIAAGWVKPEN
                     IEWREGQGIFIKPEWTPQAQKHIDDREYAYLSAYMHYFADTGEPYLLRMASLTNDPGI
                     TGMNPVAALSADDLYVVSPSQEQSPMNEQLRQLLTALGLTVADGDEFTPELGTAALSA
                     LTGIKTRADAHDTLKTQVASLSAELETAKGTPTGGTIDLTKYVPVETYNALRTEYAAL
                     SAQHGSTTLEQLLDKAESEGRIFKSERGYMEGLGQQIGVAALSAQLDARQPVAALTTL
                     QTDTVTVPDKKTATAVLSAEDIAAARLLGKTEAEFLKMKEEMQ"
     gene            53232..54179
                     /locus_tag="ACHKFK_000842"
     CDS             53232..54179
                     /locus_tag="ACHKFK_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFO9051250.1"
                     /translation="MPTPITPAMITALMTGYRSDFQAGMSMAPSQYQKIAMTVPSTSK
                     SNTYGWLGQFPQFREWIGSRVIEKMKAYGYAIVNKTFEGTVAISRDDFEDDNLGIYSP
                     LFQEMGRAAAAQPDELVFAALRDGLNAACYDGQNFFDTEHPVYPKVDGSGDAQMVSNM
                     FVAKTGSVGAQADYSGPAWYLLDCSRAVKPLIYQDRRKAELVAQTKVDEGRAFTDNEF
                     VFGASARRNVGYGFWQMAYMMQSPLTLDALWHGWSAMRDFKADGGRKLGIKPTHIVVP
                     TSLEKQAVQLLERELFADGNATVSNEMKGKLELVVADYL"
     gene            54223..54582
                     /locus_tag="ACHKFK_000843"
     CDS             54223..54582
                     /locus_tag="ACHKFK_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1506-related protein"
                     /protein_id="HFO9051251.1"
                     /translation="MNEHHTVAATDDSGLQVSGDSPVTVLAEVRCSRTHFRRAGFLFM
                     RGRQQVDVTPEQLARLEAEPCLTVRILQTPGDDAGAVAGVVHAETGADLAEAETVQDA
                     PRKKTGNNARQAKKARA"
     gene            54579..54998
                     /locus_tag="ACHKFK_000844"
     CDS             54579..54998
                     /locus_tag="ACHKFK_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp436 family protein"
                     /protein_id="HFO9051252.1"
                     /translation="MNYATETDMRARYREDLLRPLLAVPRSDEPDTRKLNRALTDASA
                     LIDSYLSSRYTLPLAVVPAVLVQHCCTIAFYYLCDQRASDQARDRYREALAWLKDVMN
                     GNVPVGVDTDGAAPESGDLPQVQSDAAVFGRNQKGFI"
     gene            54995..55558
                     /locus_tag="ACHKFK_000845"
     CDS             54995..55558
                     /locus_tag="ACHKFK_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1834 family protein"
                     /protein_id="HFO9051253.1"
                     /translation="MITETEQAYIARIREYFGNELVSVDTHPGDWSDSVLRTMLINAP
                     AIYVAWLGAGEGRTRGRLVSHWVFYVIGDMLNGREVSRPGLYQIVARLITVLDGFRAE
                     KTSPLYFEKAVNGYTETQAGSGAVMYALYFSCEEMIDPLTDISSLDDFLRHYETFTEP
                     EGTPEFKAHIRLPGATAAGENAGLSEE"
     gene            55562..55840
                     /locus_tag="ACHKFK_000846"
     CDS             55562..55840
                     /locus_tag="ACHKFK_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO9051254.1"
                     /translation="MTTIFIKPAPGCLIRDPDTMTPLSQDGEEKPFTPFWCRRLNDGD
                     VIVVDKTAEPAPAATTANTGAAAKPAGAVAEKTAAVAEKTAASDKGTS"
     gene            55840..57342
                     /locus_tag="ACHKFK_000847"
     CDS             55840..57342
                     /locus_tag="ACHKFK_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO9051255.1"
                     /translation="MINFDYIGDNNRIPLVQIEINNSMAVTGTPPQRQAVLLFGQAAM
                     KDTTIQGRGQLDVPVRITRASQARELWGRGSMIALMVEEFIAINPDTELYAIAQGAGT
                     GQSRACVMNIIGTALADGVLSVYIGGRRYLLPVSKGKKGKELVEELAKVINADTDAPF
                     TAIAAEVSGDNAEGLKGSMGVNARFIGECSAHDLRVNYYDGETTPAGLTLELVAPTQK
                     ATNPDITRSVSGMGNRQYNYIVMPYKDAANLKILADELLKRWGPVKMSDGMVWMAHTG
                     TFGTATSFGESRNDFLFTCTSIPKAPEPDYVWAASICAVCAPSLSTDPARPLQTLALP
                     ARMAPEAPDRLTREERNSLLYTGMSTVTVAAADVVQIERQVTMYRQNAYGEIDPSYLD
                     VETIYTLSYLRYSLRTFITQRFPSHKLADDGTPVRAGQNIVTPEIMKLQLISLGEEWV
                     DLGLVENLDTFKKNLLVERNTKDRNRLDVMCTPDLVNQFRFMAAQIRFIL"
     gene            57351..57716
                     /locus_tag="ACHKFK_000848"
     CDS             57351..57716
                     /locus_tag="ACHKFK_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9051256.1"
                     /translation="MSGKQYQGTATIRVNGQEYDTLEGATFSPSGFEREVVKGAKVYG
                     YREKPREATLDCKFPAGGEGSPAADEINTWTAVTIEFVADTGEVHMMTKAWSSEPASL
                     DGGGDISAKFASATSTRVQ"
     gene            57731..58207
                     /locus_tag="ACHKFK_000849"
     CDS             57731..58207
                     /locus_tag="ACHKFK_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO9051257.1"
                     /translation="MSTRKKKTAVSDEAVMEAIRDALEDGDPRTAGLAEQLANGYVDL
                     LDGLPFGESREYRVTFRELTAKDSIDAESEAERLMETRNGPVLVASPALRGIALLRRQ
                     IAAVGQIEGPLSPRQIGQLSERDLSRLMAAVSLLDSAMAGKLVAERGRPDAVPGSD"
     gene            58335..60392
                     /locus_tag="ACHKFK_000850"
     CDS             58335..60392
                     /locus_tag="ACHKFK_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO9051258.1"
                     /translation="MAKNLKASLIVDLLGNISAKSRQWSQELGAFSRSGRTGLGGLGN
                     AARRAGQETELVGSRMQRTLAGVRGSIRTVTSDFDRLQGSITGTIGRISNLYGMLAGG
                     AAVYGFNKAFIRPAAEMENYILRLNAINHGDTAKTEAVKAWAVQNAKDTTWGLAGVMQ
                     EYASSRGFGMSDREARRFITMLQDQGGYHGWSLSDAQGASLQLKQMFARQSIQAADAN
                     ILTGYGINVYQLLADKLGVNQKIIREKGEKGKLGPDSIRLLFQVMAEQAKGAQKNAMN
                     SWTGMTSMMGDVWDGFAREVMAKGPFDSLKKSLKGFLDYADAAQKSGLQDKLATQTAS
                     ALNQGFEYARDAATGFYRVIQKVRETLQALRDAGYGDALDRIGQGAQTAAKYLMYMYL
                     ASRALKVLRFAGTGALRLGATPLRYGMAMTSVLTSPFRKPQTTVPGTQPGRAGRFLNF
                     LTGVNPAAVQPVLVTNWPAGGLASGGGDVVVSGDGKTVRGRKKRGPGRGRGVTTVVTA
                     GEQLAESAGKQGFFGRMMSRAGGLLTAAGNRMGLGRFAGLFRGAGRLGGGALWAGAMA
                     APVLLDSSASAADKAGAVGSLAGSIAGGALGAAAGPVGVAIGSTVGSYLGDYLGGWLT
                     QAWQKLRGGSDENGGQATAKTAARVELVAPEGWRARSIDVDDTAQHGLDVNVWNGGNY
                     GLY"
     gene            60379..61737
                     /locus_tag="ACHKFK_000851"
     CDS             60379..61737
                     /locus_tag="ACHKFK_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO9051259.1"
                     /translation="MASTDTPGRGSFRGVPFLVYQEQRERGGRNIVRREYPLRESGGA
                     DDLGPKLPEFTFTVLVTGDDLQTQRSRLRDALRAPGAGELMHPDYGTLNVLINSFESR
                     YNASEQGTVEFTINVIPASDDTAPTVAEDTAAALEQKSGSAMNQLFNTLSDGWTVISD
                     GLHDVQAMTDTISDKIDALENAVSGMGIVQDISAFTASFTALKGNAAALINAPRRMAE
                     SLAGMFAVLTGLPGDPSLSLTGKAGTPSGSLTTNRDSMAEQAMPQLYRTLSSLRYTLS
                     EQDDPQRLIGLTPAAQKNIRLLRSVMQSATLVSQAQTLGKLLDQVIRQNTTHVQDTDR
                     SGGLAWLESSADVQRINRDLSDAMEQQVLDLSAQGYTGTALALRDAGLALTEDLTTRS
                     VHLPGAFQVMVRTTEPALVTLYRATGNSSRWQYFVRRNNIPDPVFVPGGRSVEVISEQ
                     QD"
     gene            61721..62845
                     /locus_tag="ACHKFK_000852"
     CDS             61721..62845
                     /locus_tag="ACHKFK_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO9051260.1"
                     /translation="MSSRIELYIGGSIFSGWLTVSVRRSLEHLAGSFELGLMLPGERI
                     PSALRTGQSLTLRINGQTVISGWLDQVSQRISATRHQISISGRDKTGDLVDCAAIHPG
                     SQWRNRTLAQIAADLCAPFGIAVRWQVNDDTAARPFSSFTLENSETVADALTRAARHR
                     GVLVTSNADGDLVFTQAGSQQTDRLVLGDNLLDADYNTDWRGRYSEYRVRGHGRGGGK
                     RGDSESAARLAAPVGVISDEQIGRYRSKIILADQQTDTTGARQRALREMRRAIARSER
                     FSATVRGWFRDDGRLWDVNLLTGVSALRFGIEQTELLVCQVEFLLDEHNGEVTRLVLA
                     PRDGFIVPAEPDSKGRGGSGDDVDAFIRQQMKKQGISFDE"
     gene            62835..63449
                     /locus_tag="ACHKFK_000853"
     CDS             62835..63449
                     /locus_tag="ACHKFK_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO9051261.1"
                     /translation="MMNDEVFSRLIAPVTRGIRLLFGRGVLTGTHDELKMQNVQLTGM
                     DGETFDDVERPQQYGQISVPLPGAETFFACLGGQRDQTVVLVVEDRRSRPTGLTAGDT
                     GVYHHEGHRIRLTKDGRIIVTCKMLEIYADEGMRVDTPEATFTGNVTVDKNLHVKGNF
                     VLDGTGKSDGLFTMSDAVIAGITYSGHVHQDNGKGSKTGAPENG"
     gene            63442..63879
                     /locus_tag="ACHKFK_000854"
     CDS             63442..63879
                     /locus_tag="ACHKFK_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO9051262.1"
                     /translation="MADIAIVWDQGCGSLQLNGADLLTDDSLLTAVLISLFTDRRALA
                     SDEIPDGTRDRRGWWGDSFRPRPIGSRLWLLRREKTLASVISRARAYADEALGWLSQD
                     GVASSVVCHAERVGHARLALSVRITLPDGSVRPMIFYADLKGE"
     gene            63879..64961
                     /locus_tag="ACHKFK_000855"
     CDS             63879..64961
                     /locus_tag="ACHKFK_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313014.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO9051263.1"
                     /translation="MPYQPLPLAQLITQTQQDISQRLPGSQPGVNETTLNAIAYAQAG
                     LSAQEHEHLAWIARQIIPTEADEAELLKHCAFWGVMRKPASRGDGPVQLMLTTDAGIK
                     EGVLLQRSDGVVYRITTSLTGKAGTMNVSVEAESAGRAGNAPAGTKLTFITPQAGINQ
                     TATVTGTGITGGADVETVPELLSRLVFRVQNPPSGGTQYDFERWAREVPGVTRAWCRP
                     EWPQAGSVGVTFVQDNNPDIFPGDGDVQRVADYIRSHDDPATGQPVGQPLGPTVTVFK
                     LTNKPVPFSIRIIPKTPENQAAVKQALTDLLYNESRPGGLVLPSSFWRAVAGVKNLED
                     FEVRSPLTSVQAGDSELLTVGEITWL"
     gene            64952..65512
                     /locus_tag="ACHKFK_000856"
     CDS             64952..65512
                     /locus_tag="ACHKFK_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative phage tail protein"
                     /protein_id="HFO9051264.1"
                     /translation="MAVTLTPHQRALLQLLPDGLAWDKRPSSVLASLCLGLSHSTARV
                     SWTGKQLLAERFPDTSRLLLEDWERYLGLPECDMAGATLTERQRYAGNKYRMKPSLNR
                     EFYIRFAAEFGYQIDIQPSPDSQWVSIVTINSETGYRNMNVLDDILTPLRIYEGGALE
                     CILNRYKPAWQTFIYVYANSHEEETI"
     gene            65512..66393
                     /locus_tag="ACHKFK_000857"
     CDS             65512..66393
                     /locus_tag="ACHKFK_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9051265.1"
                     /translation="MFHVDNNSGVANMPALAPAQSNTTTWFTEGDGQKGISWIGQDWL
                     NILQAELLNILAEASIQPDKAQLNQLTLSIKAIIAANAFSRKNNLKEIADAGAEAQRL
                     ARGYLGLGTLATKNSLGPADVNALAKDQNLADLENKGTARNNLDVYSKSEGDNRYLRR
                     EQNGADIPDKGAFIDNVGLRETVNRAANALPSDGTAVAANRLANAHTINGVPFDGTQD
                     ITISSGTVTAIRLGSVTAHMPGTWESWDLNLGGGNVLTGIKVQDVGKNTADNVGGVYY
                     RPLQYLLNGAWVTAASI"
     gene            complement(66425..>66907)
                     /locus_tag="ACHKFK_000858"
     CDS             complement(66425..>66907)
                     /locus_tag="ACHKFK_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO9051266.1"
                     /translation="MPYGNNVQYFQSEDGKDFYESLPLFTKKYKLCITPDSGVICSIS
                     QDASALYPAGFSVVEVDELPEGTDISGNWKFDNGIISRIPVNYARKLEAMRQSYLNQA
                     YEKINDWRTELQLGTISDEDRAALTQWMAYISQVKKMELPAIKTEAEFNAIKWPEQPQ
                     "
BASE COUNT        16372 a        16330 c        18443 g        15762 t
ORIGIN      
        1 ggtatgaccg tcgcggatgc ggattcgatt ggcattcaga tagacggcga caaggcggtc
       61 gtgaacaacg acggtgacaa tgccatcagc aacggcggca ccggcacgca ggttaacggc
      121 gacgaagcca ccgtgaacaa taacggcagc accaccgttg acggcaagga ttccaccggc
      181 accgagatcg caggtaataa cgccaccgtt actcaggaag gtgaactcac ggttagcagc
      241 ggcggtcgcg gtatcgacat cacaggcaac aatgccaagg tcgacactaa aggcaaaatg
      301 accatcacag gtactgactc ggttggtgtg tccatcaatg gcgacagtgc gacgttgacc
      361 aatacgggcg atattgacgt cagcaattcg gctaccggat tcagcctcgt aaccaatgag
      421 gggattatct cgctggctgg cagcatgaaa gtgggcgatt tttcaacggg catggcgctt
      481 agcggcgaca acaacagcgt gacgctggcg gcgaaagata tcaatgtcac cgggcagaaa
      541 gcgacaggcg ttaacatctc aggcgagagt aataccgttg atatcaccgg taatattctg
      601 gtggataaag accaaaccgc agataacgcg gtagtttatt tctacgaccc atccgttggt
      661 gtgaatatca gcggcaacag taacacggta tcactggacg gtaagctaac cgttattgca
      721 gatagtgagt taacctcacg caaatacatg gagtttgatg gtagccagga gaatatttct
      781 gggcttaccg tgtctggcga tggtaacacc gttaatttga atggcggcat tcagtttgtg
      841 ggcgagaaaa acgcgctggc agacggttca actattgccg ataagcgcag ctactttggt
      901 aagacacctc ttgttagtgt cgatggacaa tccaaagttt atctgaacgg tgattctact
      961 atcagcggct ctttgccgtt aggctatgcc aacattcttc agctaagcaa taaggcggcg
     1021 cttgaaattg gtagtgacgc tacgttcagt atgcaggaca ttagtgttta cgagcactat
     1081 ttcacgcaaa ctccgcaaat tatcaaggtt gatacgggtt cacaggtcgt caacaatggt
     1141 gatgtggata tctggaatat cagttttgca ggcatttggg gcgaaaactc taccggtatt
     1201 aacaacggaa atattacgct ttctcaatat gactattctt ctccggagac atcgttctca
     1261 gaacctgacc acatggcctt tttaagctcc agtggcggta gtgctgtgaa caacggaacg
     1321 atcactgcaa aagtgatgga acagcacagt gttctcaatc tgggctctgc agccggtgtt
     1381 gcagacccga gggtattcaa taatagcgtg tcgagtatga tgggaatgga ggcctacggt
     1441 aaaggcactg ttcttaacag cgaaagcggc gttattgaca tgtacgggcg cggcaacatt
     1501 ggtatgttgg cagttgatga ctctgcagcg gataacgccg ggaaaatcac gctggatacg
     1561 ctttgggtcg atcaaaacga caccacgacg ctgcgtactg acctcccttc cagcacggct
     1621 atagattacg gtgttggtat ggccaccgga acgaatagtg gtggtggcgc aagaagcaac
     1681 ggtgttgcga ctaaccaaca aggcggtgta atcactgttt ataatgccgg tgctgctatg
     1741 gctgcttacg gtgccagcaa catggttatt aaccagggca ttatcaatct ggagaaaaac
     1801 ggaaattacg atggcggctt gggcgcgaat atgctcgtgg ggatggcggt ctataacaga
     1861 ggcaccgcta tcaacgataa aacaggcgtg atcaatatta acgtcgatac ggggcaggcg
     1921 ttttataacg acggtacagg gacaattctg aactatggtg agataaacct tctcggctcg
     1981 ccaatggaca gtgcagactc tcacatgggg gctattccgg agaatctgga tctgctgaca
     2041 gcactgacag gcagcggcga aacggatatg cgaactgctt caagtggcgg gtttgtgacg
     2101 accaaagcgc tggcaaacta cggcaatgaa acgctgaata gcaatgtggc tgccaaagcg
     2161 tggctttata accaggataa agccaacctg acaattaacg gagagctatc catcgggcaa
     2221 gggctggaaa acagcggctt actcgattct gacaccatta gcgcagccgc gaatgtctat
     2281 aaccgagcat cgggtagcat tatcactgat cagttatcgc taacgggtag taactcgttt
     2341 ttcaatgagg gcaacttcag cggttcggtt gctggctcaa gctataagca aaatgtggtt
     2401 aacacgggaa cgatggctgt aatggccgac ggtaagtcgc ttatcagtgg cagcttcctg
     2461 ctttataacg aagcaggcgc aacactcagc aacagcagca gcgccgtttc aggtggagaa
     2521 aatgccattg ttaacgtaac ccgcaccggc gattcactcg cgcaagttaa ccgtggcacc
     2581 attactgcgg tcaacggcta cagtgcaatt aaaacggcaa gtacgggttc taacagcaac
     2641 gggaaatgga tctggaatac cgacacgggg gtaattagcg gcgtgaatcc aaatgcaccg
     2701 ttaattgatc ttggtcgcgg ctataacttt gccaacgccg gcactattaa tgtgcagggt
     2761 gatggtgcag tcgctatcag cgggggaacc accagctata cggttcaact ggtgaacagt
     2821 gggaccatca atgtcggtac ggcgcagggt aaagcggacg gcactaacgg tactgggtta
     2881 attggtatca aggggaatgg cagtgatacg acaattaata acgcacaaag cggtgtaatt
     2941 aacgtttatg cagataattc ctgggccttc ggtggaaaga ccaaagcgat cattaacaat
     3001 ggtgaaatta acctgctgtg tgatacggga tgtgatatct atgcaccggg taccaccggt
     3061 acgctgaacg atcataacag tactactgat attatcgttc ctgcagcaac gagcacacct
     3121 acgcagggtt cagtccccac agtacctgcg gactccagtg cacagcaaaa actgactaac
     3181 tacactattg gtaccaatag cgatggcacc tctggtatgt tgaaagccaa taacctggtc
     3241 atcagcgaca acgtcaaggt aaataccggg tacagtgcag gtactgccga tacgactgtt
     3301 gtgattaatg atgttttcaa aggtgaaaat atttccgggg ctgagaatat ttcttcttcc
     3361 acggtaatgt ggaatgctca gggcagcact gatgccagcg gcaacgtaga cgtcacgatg
     3421 accaaaaacg cctatacgga tgttgtgacg gattcttcag ttaataatgt cgctcaggta
     3481 ctggattcgg gttacaccaa taacgacctg tacactagtc tgaacgtagg aacgactgct
     3541 gaactgaaca gtgcgctgaa acaaattagc ggcagccagg caaccacggt attcaatgaa
     3601 gcacgagtgt taagtaaccg cttcagcatg ctgtccgatg cagcaccgga agttgcaaac
     3661 ggtctggcct tcaacgtggt agcgaaaggc gacccgcgtg ctgaactggg aaataataca
     3721 cagtacgaca tgatggcgct gcgtaaatcc atgacactga ctgaatatca gaatctgagt
     3781 ctggaatacg gtattgctcg tctggagggt aatggttcag ataccgtagg cgataatggt
     3841 gtgactggcg gttacagcca gttcttcggt ctgaaacacc agatggcgtt tgacaacggt
     3901 atgagctgga ataacgccct gcgttatgat gtgcataatc tcgatagcag tcgctcgata
     3961 gcttacggcg acgtcaacaa aaccgctgat gctaatgtga aacagcagta tctggaattc
     4021 cgtagtgaag gggcaaaaac ctttgaactg cgtgaaggtc tgaatgtgac gccttatgcg
     4081 ggtgtgaaac tgcgtcatac gctggaaaac ggttatcagg agcgtaatgc gggcgacttt
     4141 aacctgagca tgaacagcgg cagcgaaacg gcggtggaca gcatcgttgg gctgaaactg
     4201 gactatgcag ggaaagaagg ctggagtgcg aacgccacgc tggaaggtgg gccgaatctg
     4261 agctatgtga aaagtcaacg tactgcgagt atttcgggcg cgggaagcca gcgtttcaac
     4321 attgacgatg gtcagagtgg cggtggtttc aatagtctgg cgacaatggg tgtgaaatac
     4381 agcagccagg aaagtgcatt gcagttagat gccttccact ggaaagaaga cggcatcagt
     4441 gataaaggtg tgatgctaaa cttcaagaaa acgttctaat ttttagcatg taatacataa
     4501 accgcaacgc tgatacaggt tgcggttttt ttattgccgg atgcggtgct tgacgtgttt
     4561 tgttttggtg tctttcagga tattagagct actcatccag taatcttgtt tacgccttcg
     4621 cattatttat ctctattcat ttctatactg atttttctta atccgtttta ttacaggaca
     4681 gggtgcgatg agcagcaaca catttactct cggtacaaaa tccgttaacc gtcttggtta
     4741 tggcgcgatg caactggcag gtcctggggt ttttggcccc ccaagagatc gccacgtcgc
     4801 tataaccgtg ctgcgcgagg cgctggcatt gggcgtcaat catattgata ccagcgactt
     4861 ttatggtccg cacgtcacca atcagattat ccgcgaagcg ctttatcctt actctgacga
     4921 cctgacaatt gtcactaaaa ttggtgcgcg gcgtggagag gacgcttcct ggttgcccgc
     4981 attttctccg gcagagctgc aaaaagcggt gcacaataat ctacgtaatc tcgggctgga
     5041 cgtactggat gtggttaacc tgcgcgttat gatgggggat ggtcatggcc cagcggaagg
     5101 atcgattgaa gccagcctga ccgtgctggc agagatgcaa caacaaggcc tggtaaaaca
     5161 tattggtctg agcaacgtta caccgacgca ggttgcagag gcgcgtaaga ttaccgaaat
     5221 cgtctgtgtg cagaacgaat acaacattgc tcaccgtgct gatgacgcaa tgattgatgc
     5281 tttggctcgt gacggcattg cctacgtgcc gttctttccg ctcgggggct ttacgccgct
     5341 gcaatcctcc acgctttccg atgttgctgc gagcctgggt gcaacaccaa tgcaggtggc
     5401 gttggcgtgg ctgttacagc gttcaccgaa tattttgctg attcctggaa catcttctgt
     5461 tgcgcattta cgggagaata tggctgctga aaaaattgca tctttctgag aaagtgttgt
     5521 ctacgttgga tggtatttcg cgagaataac gaatatacaa aagggaaaga tgcatttccc
     5581 ttttgtttct tttttaatgg catggagtgc atatgtttaa ggccaggatt tgcggctgga
     5641 taatcaggga aaaggcgatg gattcattga ttcgtacagt gatagatggt atctcactga
     5701 tatccagacg gtaatcaaac agatgtgtag ggcattcatc caccacatct tgatatattc
     5761 cgaatttacg ttgatgctgc gcgttgtgat atctccgccg tcggaattga tgcaccatag
     5821 agcaaactga atatcacgga atgataaatg acgctctacc agataaaacc gctctttcag
     5881 tcgctgttaa ggccgacgat gttttggctt tataagcacc atgttacagc gaatcacatc
     5941 actcttgctg cactggcgct ttctcttctt accggattgc tgttggtgtt ggtggcacaa
     6001 cccatcctct ttttgctatt gcccatcgtg ctttttatcc gtatgacgct caatgcgctg
     6061 gatggcatgt tggcgcgtga gtgcaatcag aaaacacgtc tgggggcgat tttgaatgaa
     6121 actggcgatg ttatttccga tattgtgctc tatttaccgt ttttattttt accggaaagt
     6181 aacgcatcac tcgtaatact catgttattt tgcaccatat tgactgagtt ttgcggttta
     6241 ctcgcgcaga cgattaacgg tattcgcagt tatgccggtc catttggcaa aagcgatcgc
     6301 gcgttaatat ttggtctgtg ggggctagcc gttgccattt atccacagtg gatgcaatgg
     6361 aataatcttt tatggagcat tgcttcaatt ctgcttctct ggactgcgat taatcgttgt
     6421 cggagtgtgc tttttatgag tgcagaaaga taatgctgga aaaatctctg gcaacacttt
     6481 tcgcgttgtt aattttagca acgctgataa accgttttct ggtatggcgg ttaccggaga
     6541 gaaaaggggg tgaggttaca ttacgtattc gtacctggtg gggcatcgtc atttgttttt
     6601 caatggtgat ttcaggtcca cgctggatga cgttgacgtt ttttgcgctg ataagttttc
     6661 tggcattgaa agaatattgt acgcttatat ctgtacattt tccgcgttgg ttatattggg
     6721 gtattcctct taactatttg cttatcggtt ttaattgctt tgagctgttt ctgttattta
     6781 tacctttggc tgggtttctg atattagcca cctggcgagt tttagtgggt gacccctccg
     6841 gttttctgca taccgtgagc gccatttttt gtggctgggt aatgaccgtt ttcaccttga
     6901 gtcatgccgc ctggttatta atgttgccaa ccataaatat ccagggcggg gcgttactgg
     6961 tgttatttct tttggcgtta accgagtcaa acgatattgc acagtattta tggggaaaat
     7021 cctgcggcag aagaaaagtg gtccccaaag tcagcccggg aaaaacgctg gaaggtctgg
     7081 tggggggagt aatcaccacc atgattgcgt cactgattat tggaccgtta ctgacaccgc
     7141 taaatacatt acaggtatta ttagcgggtt tgttaattgg tattagtggt ttttgcggcg
     7201 atgtagtgat gtcagccacc aaacgagatg ttggtgttaa agatagcgga aaactattac
     7261 caggacatgg cggactcctt gacaggattg actcattaat tttcaccgct ccggtatttt
     7321 tttattttat tcgctactgc tgttactgaa ggaaaataga gaaaatggaa aattcacgca
     7381 tccctggggg acattttttt accaccagtg ataatacagc gttgttttat cggcactggc
     7441 ccactttaca gccaggggcg aaaaaggtca tcgtcttatt tcatcgcggt catgaacatt
     7501 ctggtcgtct acaacatatc gttgatgaac tggcgatgcc agatactgct ttttatgcat
     7561 gggatgcccg agggcatgga caaacttcgg ggccgcgtgg ttatagtccg tctcttgcgc
     7621 gttcagtgca ggatgttgat gaatttgtcc gttttgctgc cagcgacagc caggtcggac
     7681 tggaagaggt ggttgtgatc gcgcaaagcg tcggcgcagt gatggttgcc acatgggttc
     7741 atgattatgc gcctgctatt cgcggactag tgctggcatc tccggccttt aaggttaaat
     7801 tgtatgtgcc gctggcacgt cctgggctgg cgttatggca tcgtctgcgt ggtctgtttt
     7861 ttattaattc ctatgtgaaa gggcgttatc tgacccacga tcggcaacgg gtggcgagtt
     7921 tcaataatga tccgctgatc acacgggcga ttgccgtcaa tatcttgctc gatctctata
     7981 aaacgtctga acgtattgtt agcgatgcgg cggcgattac gctctccacg caacttctga
     8041 tatcaggcga tgactatgtg gtgcatcgcc aaccgcagat tgatttttat cagcgtttac
     8101 gtagtcctct gaaagagctg catctgctgc caggctttta tcacgacacg ttgggtgaag
     8161 agaacagggc gcaggcattt gaaaaaatgc aaagctttat cagccgttta tatgctaaca
     8221 agtcgcaaaa atttgattat cagcatgaag accgcacagg accatcagcg gatcgatggc
     8281 ggctcctttc tggtggaccc gtgccgttat cgccggttga tttggcgtat cgctttatgc
     8341 gaaaggcgat gaaactgttc ggggtgcact ctgcgggcct gcatctcgga atgagtaccg
     8401 gctttgattc aggcagttcg ctggattatg tctatcaaaa tcaaccgcaa ggcagtaacg
     8461 cattcgggcg ctttatcgac aaaatctacc tgaacagtgt tggctggcgc ggtattcgcc
     8521 agcgcaaaac ccatttacaa atgctgatta aacaagccgt tgcccatctc cacgccaaag
     8581 gtttagccgt ccgcgtggtt gacattgccg cagggcatgg gcgctatgta ctggatgcgc
     8641 tggcaaacga gcccgccata agcgaaattt tgttacgtga ttacagtgag gtaaatgttg
     8701 cacaagggca agagatgatt gcccaacggg gaatgtctgg gcgggcgcgt tttgaacagg
     8761 gcgatgcgtt taatccggca gaactcagca cgttaactcc gcggcctacg ctggcgattg
     8821 tctctggcct gtatgagctt tttcccgaaa atgagcaggt aaaaaactca ctcgcaggtc
     8881 ttgccaatgc catcgatccg ggtggcattc tcatctacac cgggcagccg tggcacccac
     8941 aactggagct gattgccggg gtgttaacca gtcataaaga tggtaaaccg tgggtaatgc
     9001 gcgtgcgttc tcaaggggag atggattcgc tcgtgcatga taccggattt gataaatgca
     9061 cacaacggat tgatgagtgg ggcattttta cggtttcgat ggcggtgcgt cgtgataact
     9121 gaacgccgga acgtattgct acaaggagct ggctggttat tgttgctggc cccgtttttc
     9181 ttctttacat atggatttct taatcagttc accgcgactc aggatcttaa caaccatgat
     9241 atccccagtc aggtattcgg ttgggaaacg gcgatccctt ttcttccctg gactattgtt
     9301 ccttactgga gtctggatct tttatatgga ttttcgctgt tcatttgtag ctcgacattc
     9361 gaacagcgcc gactggttca ccggcttatt ctggcaacgg taatggcctg ctgcggtttt
     9421 tttctctatc cgctgaagtt tagttttatc cgtcctgaag tgagtggggt gacaggatgg
     9481 ctattttcgc aacttgaact gtttgatctg ccttataacc agtctccttc gctgcatatt
     9541 attctctgct ggctactttg gcgtcacttt cgtcagcatc tggctgtgag gtggcgtaaa
     9601 gtctgcggcg gatggttttt actcatcgcc atttcgacgc tgacgacctg gcagcatcat
     9661 tttattgatg tcatcacggg gctggcggta ggtatgttaa ttgactggat agtgcccgtc
     9721 gaccgtcgtt ggaattatca gaaacctgat caacgtcgaa tcaaaatagc actgccatat
     9781 gtcgtaggcg cgtgctcgtg cattgtgttg atggagctaa tgataatgct tcagttatgg
     9841 tggtcagtct ggttatgttg gccagtatta tcgctattca tcattggccg tgggtacggt
     9901 gggcttggcg cgataacaac agggaaagat agtcagggga aactcccgcc cgccgtttac
     9961 tggctgacat tgccctggcg tatcgggatg tggctgtcta tgcgttggtt ttgtcttcgc
    10021 ctggagccgg tgagcaaaat tactgctggt gtttatttag gggcgtttcc acgacatatt
    10081 ccggcacaga atgcggttct ggacgtcacc tttgaattcc ctcgcggacg agcgacaaaa
    10141 gatcgactct atttttgtgt accgatgttg gatctggtgg ttccggaaga gggggagctc
    10201 cgacaggccg tggcgatgct ggaaacatta cgcgaagagc aaggcagcgt tctggtccat
    10261 tgcgcgttgg gattatcgcg cagtgcgctg gtggtggcgg catggctgtt atgttacgga
    10321 cattgtaaaa ccgttgatga agcgattagt tatattcgag ccagacgctc gcggattgtg
    10381 cttaaggaag agcacaaagc gatgctgaaa ttatgggaaa acaggtaagc ggattgagat
    10441 gtggactgaa tatctacagt ccacatcaag atcgtgtccg gttattcagc aacaatactg
    10501 tcgatggctg ctttcgcgtc agactgcgct ttcgctgcca tttccgggcc gtacgcgatc
    10561 ccttcggcga agacaaattt cacatcggta atgccgataa agcccaggaa tgtggacaga
    10621 tatggcgtta ccagatccgt tgggccatct ttatggatcc caccgcggct ggtgataacg
    10681 atggcttttt taccggtgac caggccttcc ggaccgttct cggtatagcg gaaagtaacg
    10741 cctgcacgtg caaccaggtc aaaatagttt ttcaactggg tcgagatatt gaagttatac
    10801 atcggtgccg caataacgat aacgtcgtag gcttttagct cggcaatcag ctcatcggaa
    10861 agcgccagag cttcctgctg acgcggagtc agcggcgcat cgctcggacg cagtgcacca
    10921 accagttcgc catccaggac cggaatcgga tttgcagcca ggtcacgcac ggtgatttca
    10981 tcagtggagt gcttttcgcg ccattgttca acaaaataat cggacaactg attagactga
    11041 gagtaccctg ccaggatgct ggatttaaga actaatacct tgctcatggt gtttccttat
    11101 agatatttga atgggcaatg ccccgttgct tgttgacact ttattcacaa ttctgccaca
    11161 gagatagcgc aataaatcga agcctatgtt cgaatttatt gaacaacgca tagaaagccg
    11221 cgatgtggta ctctatatct atcatttaaa agaaaattaa tcaggcagac tactgcccac
    11281 taacgttatg acagaacaac aaaaattgac ctttactgcc ttgcagcagc ggctggattc
    11341 gatgatgctg cgtgacagac tgcgtttttc tcgccgtctg cacggcgtga agaaggttaa
    11401 aaatcctgat gcacaacagg ccattttcca ggagatggcg aaagagattg accatgcggc
    11461 agggaaagtc ctgctgcgtg aagcggcacg accggagatt acttacccgg acaatttgcc
    11521 ggttagtcag aaaaagcagg acattctcga agcgattcgt gaccaccagg tggtgatcgt
    11581 cgccggggaa acgggttccg gtaaaacgac tcagctaccg aaaatctgta tggagctggg
    11641 tcgcgggatt aaagggctga tcggccatac ccagccgcgt cggctggcgg cgcgaacggt
    11701 ggcgaaccgt attgcggaag agctaaaaac ggagccgggc ggttgcatcg gttacaaagt
    11761 gcgtttcagc gatcacgtca gtgataacac gatggtcaag ctgatgaccg acggtatcct
    11821 gctggcagag atccagcaag accgcctgct gatgcagtac gacaccatca ttattgacga
    11881 agcgcacgaa cgcagcctga atatcgattt tctgctcggc tatttgaaag agttgctgcc
    11941 gcggcgtcct gacctaaaaa ttattatcac ttccgcgact atcgacccgg aacgcttttc
    12001 gcgccacttt aataatgcgc cgattatcga agtctccggt cggacctatc cggtggaagt
    12061 gcgctatcgc ccgattgttg aagaagccga tgataccgag cgcgatcagt tgcaggcgat
    12121 ttttgacgcc gtagatgaac tgagccagga aagccctggc gacattctga tctttatgag
    12181 cggcgagcga gaaattcgcg ataccgccga tgcgctgaac aagctgaact tacgccatac
    12241 cgaaatcttg ccgctttatg cacggctttc caacagcgaa cagaaccgcg tgttccagtc
    12301 gcacagcggg cgacgcattg tactggcgac caacgtcgcg gaaacctcgc tgaccgtacc
    12361 gggtattaaa tacgttatcg acccaggtac ggcgcgtatc agccgctaca gctatcgcac
    12421 caaagtgcag cgtttgccga ttgagccaat ttcgcaggct tcagctaatc agcgtaaagg
    12481 ccgctgcggc cgtgtgtcag aagggatctg tattcgtctc tattccgaag acgatttcct
    12541 ctcgcgcccg gagtttaccg acccagaaat tctgcgtact aacctggcct cggttatttt
    12601 gcagatgacc gcactgggct tgggcgatat cgccgcattc ccgtttgtcg aagcaccgga
    12661 taaacgcaat atccaggatg gcgtgcgtct gctcgaagag ctgggcgcga tcaccactga
    12721 tgaacaggcc agcgcctata aactgacgcc gctcggtcgc cagctctcgc aactgccagt
    12781 cgatcctcgt ctggcgcgaa tggtgctgga agcgcaaaaa catggctgcg tgcgtgaggc
    12841 gatgattatc acgtccgcgc tctctattca ggacccgcgt gagcggccaa tggacaagca
    12901 gcaggcatcg gacgaaaaac atcgtcgctt ccacgacaaa gagtctgact tcctcgcgtt
    12961 tgtgaatctg tggaattatc ttggcgagca gcaaaaggcg ctctcttcaa acgccttccg
    13021 tcgcctgtgt cgtaccgatt atctcaacta tctgcgcgtg cgcgaatggc aggatatcta
    13081 cacccagttg cgccaggtgg tgaaagaact tggcattccg gttaacagcg aaccggcgga
    13141 ttatcgcgaa attcacattg cgttgctgac cggtttgctt tcgcatatcg gcatgaaaga
    13201 tgccgataaa caagaatata ccggcgcacg taacgcgcgt ttctccatct tccccggttc
    13261 tggtttattc aaaaagccgc cgaaatgggt aatggtggcg gaactggtag aaaccagccg
    13321 cctgtggggg cgcattgcgg cacgcatcga tccagaatgg gtggaacctg tcgctcagca
    13381 tttaatcaaa cgcacctaca gcgaaccgca ctgggaacgg gcgcagggcg cggtgatggc
    13441 aacggaaaaa gtcaccgttt acgggttgcc gattgttgct gcgcgtaagg tcaactacag
    13501 ccagatcgat ccggcgttat gtcgtgaact ctttattcgc cacgcgctgg tggaaggtga
    13561 ctggcagacg cgtcacgcat tcttccgtga aaacctgaaa ctacgggccg aagtggaaga
    13621 actggaacac aaaactcgtc gccgcgatat tctggtggat gacgaaacgc tgtttgagtt
    13681 ctacgaccag cgcatcagcc acgatgtgat ttccgcacgt cacttcgaca gctggtggaa
    13741 aaaagtcagc cgcgaaacgc ctgatttgct caactttgaa aagagcatgt tgatcaaaga
    13801 aggggcggaa aaaatcagca aactggatta cccgaacttc tggcatcagg gcaatctcaa
    13861 actgcgtttg agctatcagt ttgagcccgg cgcggatgct gacggtgtga ccgtgcatat
    13921 cccgctgccg ttacttaacc aggttgagga aagtgggttt gagtggcaga tccccggtct
    13981 gcgtcgtgag ctgatcatcg ccctgattaa atcgctgccg aagccggtac gccgtaattt
    14041 tgtgcccgcg ccaaactacg ccgaagcgtt tttaggccgc gtcacaccgc tggaattgcc
    14101 gttgctcgac agccttgaac gagagttacg gcggatgact ggcgtcaccg ttgaccgcga
    14161 agactggcac tgggatcagg tgcccgatca cctgaaaatc actttccgcg tggtggatga
    14221 caaaaacaag aagctaaaag aagggcgctc attacaggat ctgaaagacg ccctgaaagg
    14281 caaagtgcag gaaacgctgt ctgccgtggc ggatgacggt atcgagcaga gcggtttaca
    14341 tatctggagt tttggtcaac tgccggaaag ctacgagcag aagcgcggca actacaaagt
    14401 gaaggcgtgg ccggcgctgg tggatgagcg tgacagcgta gcgatcaaac tgtttgataa
    14461 cccgctggaa cagaagcagg caatgtggaa cggtcttcgc cgtttactgc tgctaaatat
    14521 tccatcgccg atcaagtact tgcacgaaaa gctgccaaac aaagccaagc tggggctgta
    14581 ctttaatccg tatggcaaag tgctggagct gatcgatgac tgtatctcct gcggtgtgga
    14641 tcaactgatc gacgccaatg gtggcccggt ctggacggaa gaaggctttg ctgcgctgca
    14701 tgaaaaagtc cgcgctgaac tgaacgacac ggtggtggat attgcgaagc aggtcgaaca
    14761 aatcctcact gcggtattca atatcaataa acgcctgaaa ggtcgcgttg atatgaccat
    14821 ggcgctggga ctgtcggaca tcaaagcgca gatgggcggg ctggtgtatc gcggttttgt
    14881 cactggcaac ggttttaaac ggttgggcga cacgttacgg tatttgcagg cgattgaaaa
    14941 acgactggaa aaactggcga ttgatccaca tcgcgaccgt gctcagatgc tgaaagtcga
    15001 aaacgtccag caggcgtggc agcaatggat caacaaacta ccgcctgcac gtcgtgagga
    15061 tgaagacgtg aaagaggtcc gctggatgat agaagagctg cgcgttagtt acttcgctca
    15121 acaacttggc acgccttatc cgatttcgga taagcgtatt ttgcaggcga tggagcagat
    15181 tagcggttaa cactgctatt tgcctgataa atctaaaacc cggtaagcat ttagcgccgg
    15241 gttttttatt aattctaaaa acgataaggg aaaaaattca ggagttcaga aaaatacaat
    15301 tctctgctgc aagatgaata atgtttatct acagcatttc cttaaaaaat atgtcaggct
    15361 tgcggagtgg ctgttaagga aatacgactt cctcctttca gagtgctccg cttctcacta
    15421 ttatctcacg cagtattctt aagggaacga taaggaggaa ccatgaatat taccccgttt
    15481 ccgacacttt cgccggcaac catagatgcc ataaatgtta tcggacagtg gctggtgcag
    15541 gatgatttct ccggtgaggt accgtatcag gccgattgcg tgatccttgc aggcaatgcg
    15601 gttatgccga ctatcgatgc ggcatgtaag attgcccgcg atcagcaaat tcctttactg
    15661 attagtggtg gtatcggtca ctcgacaact tttttgtata gcgccatcgc acagcatccg
    15721 cactacaaca ctatccgcac cactggcaga gctgaagcta ccatcctggc ggatatcgct
    15781 catcagttct ggcatattcc gcatgaaaaa atctggattg aagaccagtc aacaaactgc
    15841 ggtgaaaacg cacgctttag catcgcgcta ttgaatcagg ccgtagaacg agttcatacg
    15901 gctatcgttg ttcaggaccc caccatgcag cggcgcacga tggcgacgtt ccgccgtata
    15961 accggggata atcccgatgc accgcgctgg ttaagttatc ccggattcgt tcctcagtta
    16021 ggaaataacg cagacagtgt aatctttgtt aatccgttgc aaggattatg gccggttgag
    16081 cgttatctat cgctactcac tggcgagttg ccgcgtttac gcgatgatag cgatggctac
    16141 ggcccccgcg ggcgagattt tatcgttcac gttgattttc cggcagaagt catccaggca
    16201 tggcaaacgc tgaaacacga tgcggtgctc atcgaggcga tggaaagtcg ctcgttacgt
    16261 taaaaattgc ccgtttgtga accacttgtt tgcaaacggg catgacttct gacttttatt
    16321 tctgccttta attcctttta cacttgtttt tatgaagccc ttcacagaat tgtcctttca
    16381 cgatgccgtc tctctgataa ttgatgttaa ttgacaataa atttaccaaa aacaaacatg
    16441 caaatcacag gagtcgccca tgtcagtacc cgttcaacat cctatgtata tcgatggaca
    16501 gtttgttacc tggcgtggag acgcatggat tgatgtggta aaccctgcta cagaggctgt
    16561 catttcccgc attcccgatg gtcaggccga ggatgcccgt aaggcaatcg atgcagcaga
    16621 acgtgcacaa ccagaatggg aagcgttgcc tgctattgaa cgcgccagtt ggttgcgcaa
    16681 aatctccgcc gggatccggg aacgtgccac tgaaatcagt gcgctgattg tcgaagaagg
    16741 gggcaagatc cagcagttgg ctgaagtcga agtggctttt accgcggact atatcgatta
    16801 tatggcggag tgggcacggc gttacgaggg cgagattatt caaagcgatc gtccaggaga
    16861 aaatatcctt ctgtttaaac gtgcgcttgg cgtgactacc ggcattctgc cgtggaactt
    16921 tccgttcttc ctcattgccc gcaaaatggc tcccgctctt ttgaccggta ataccatcgt
    16981 cattaaaccc agtgaattta cgccaaacaa tgcgattgca ttcgccaaaa tcgttgatga
    17041 aataggcctt ccgcgcggcg tgtttaacct tgtactcggg cgtggtgaaa ccgttgggca
    17101 agaactggcg ggtaacccaa aggtcgcaat ggtcagtatg acaggcagcg tctctgcagg
    17161 tgaaaagatt atggcgactg cggcgaaaaa catcaccaaa gtgtgcctgg aattgggggg
    17221 taaagcaccg gctatcgtaa tggacgatgc cgatcttgaa ctggcagtca aagccatcgt
    17281 tgattcacgc gtcattaata gtgggcaagt gtgtaactgt gcagaacgtg tttatgtaca
    17341 gaaaagcatt tatgatcagt tcgtcaatcg gctgggggaa gcgatgcagg cggttcaatt
    17401 tggtaacccc gctgaacgca acgacattgc gatgggaccg ttgattaacg ccgcggcgct
    17461 ggaaagggtt gagcaaaaag tggcgcgcgc agtagaagaa ggggcgagag tggcgttggg
    17521 tggcaaagcg gtagagggga aaggatatta ttatccgccg acattgctgc tggatgttcg
    17581 ccaggaaatg tcgattatgc atgaggaaac ctttggcccg gttctgccgg tggtcgcatt
    17641 tgacacgctg gaagaggcta tctcaatggc taatgacagt gattacggcc tgacctcatc
    17701 aatctatacc caaaatctga acgtcgcgat gaaagccatt aaagggctga agtttggtga
    17761 aacttacatc aaccgtgaaa acttcgaagc tatgcaaggc ttccacgctg gatggcgtaa
    17821 atccggtatt ggcggcgcag atggtaaaca tggcttgcat gaatatctgc agacccaggt
    17881 ggtttattta cagtcgtaat gagtgaaaga ggcggaggtt ttttcctccg cctctgcgcg
    17941 tcagagttta gcgaattttt cgagggtgcg aataagctgc gtgacgaagc catattcgtt
    18001 atcgtaccag gcgaccgttt tcaccagttg taaatcgccc acggcggtaa tttccgtttg
    18061 cgtggcatca aacaccgaac cgaaatggct gccaatgata tcggaagaga ctatttcttc
    18121 atcagtataa ccaaatgact cgttattgtt tgtcgccttt ttaagcgcgt tgttcacctc
    18181 ttcggcagtc actttttttc cgagaatcga caccagttca gtgaccgaac ctgttttcac
    18241 cggcacgcgt tgcgcatgac ctttcagttt gccgctcaat tccgggatca ccagaccaat
    18301 ggcttttgcc gcccccgtag tgtggggaat gatattttct gccgctgcgc gtgaagcacg
    18361 taaatcttta ccacgtgggc catccaccag tgactgggtg ccggtatagg catgaatggt
    18421 tgtcatcgtg ccgacttcta tcccgaaact gtcatgcaag gctttggcca tcggcgcaag
    18481 gcagttagtg gtgcatgacg ccacggaaac aatggtgtcg ttgtcatcca gagtgtcgtc
    18541 attgacgtta taaacgatgg ttttcatttc accggcaggg gcggaaatca acaccttctt
    18601 cgcgccagca tcaagatgcg cctgcgattt ctcggcggag gtataaaagc ctgtacattc
    18661 gacaatgatt tctgcgcctt tcgctttcca cggaatattt ttagcctctt tttcggcgta
    18721 aaccgcgata cttttcccat caacgataag tgaatcttcc gtataatcaa tgctccaggg
    18781 gaacggtccg tagtttgaat catgtttcag caggtaggcg agaatttttg gggaagtgag
    18841 atcattaata gcgacaacgt ctatgttgct tttgacttca agtaatcgac gcaacaccag
    18901 tcgaccgata cgaccaaaac cattaatacc aactttactc atggtcttct cctgtcagga
    18961 acgtttggat gaaaattgat cctttccagg cttagaccag gatggcggga tgggcaatct
    19021 acattcttgc agtgaaacgt aacgtactga aaacgggtga acaatattta atgaaatttt
    19081 aagaaaagcc cgttatgtta acggcaaatt atgttaaagc aggaaatgtt atgggaaata
    19141 aatactcaag gttacaaatc agcattcact ggctggtctt tttgctggtt atcgtagcgt
    19201 attgcgcaat ggagtttcgt ggtttcttcc cacgtagcga tcggcccctc atcaacatga
    19261 ttcatgtttc ctgtggcatc tcaattctcg tactgatggt ggttcgtctt ttgttaagac
    19321 tgaaataccc aaccccgccg attacaccta agccaaaacc gatgatgacg ggactggcgc
    19381 atttgggcca tttggtgatt tatctcctgt ttattgcgct gccagtgatt ggtttggtga
    19441 tgatgtataa ccggggcaac ccgtggtttg cgtttggttt gacgatgcct tacgcttcag
    19501 aggccaattt cgagcgggtt gatagcttaa agtcgtggca tgaaacgctg gcgaatctgg
    19561 gatattttgt catcggcttg cacgctgcgg ctgcactggc acaccactat ttctggaagg
    19621 acaacacact tctacgcatg atgccacgta aacgttcctg aaggatattt aaagaaaacg
    19681 cctgtactaa aaccgacccg tggtacaggc gaagaatacg ggcctacatc ggaagcgcct
    19741 attatattta ttcgtatgac aaataaaacc ataatccttg tctatacgtc catctggctt
    19801 atctttaatc aattcacccg atctttgatc tcatcaacgg tatcaaaata aaccagcgta
    19861 taacgttcaa atcgaacata agattcgata aatcatggag gttatatgaa aaaactggca
    19921 cttatcctgg ttatgggaac gcttgtttcc ttttatgccg atgccgggcg caaaccctgt
    19981 tctggttcga aaggggggat ctcacactgt acggcaggcg gcaaatttgt ctgtaatgat
    20041 ggttctatta gagcatcgaa aaaaacatgc actaactgaa gtgtaaaagg ggtgccatga
    20101 gaaaatgaat ccgctagtca gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac
    20161 gtgcaggcca tgagcgcagc aacttcctta tcaccgtccc ggaaccgcag ttcgtagagc
    20221 gattgtcggg tcagtgtgaa tgtcagaatc gtgttactct cgcaaggccg tggatttgcc
    20281 gttgtcgcca gcgaagtaag ccgcagcgct caggcggcga aagagattga aggcttgatc
    20341 agtgaatcag tcaggttaat tgacctggga tcggatgagg tggcaacggc agggaaaacc
    20401 atgtgcacta ttgttgatgc cgtcgcgagt gtcacacata tcatgcagga aatcgccacc
    20461 gcctcggatg aacaaagtcg aggcataacg caggttagcc aggcgatttc tgaaatggat
    20521 aaggtgacgc aacagaatgc ttctctggta gaagaggcct caacggcggc ggtgtccctt
    20581 gaagaacagg cggcacgact aactgaggcg gtggacgtat tccgtctgaa caaacattct
    20641 gtgtcggcag aaccacgagg agctggtgaa ccagttagtt ttgctacggt gtgaaaatgt
    20701 taaaggaggg atcgacagat cccttcacct ttcagaacgg cattgatttt cgaatagcgt
    20761 taatcatcaa ctggcaacca gaagagaacg tcgcatctac gcgggtcagt attccaatcg
    20821 gttcgcctgc accatgtccc ggaacaggca gggctaccag cgtggcatga cgcaggtcgt
    20881 cttttacagc gccagaaggg acaaaccaca cgtaatcgta ttcaaccgta agttgacgag
    20941 atagcgaagc agacagcgtt tcgatacaac ccgaaggaat tttacatccc tggctctgca
    21001 ctaatgcatc tgaatgctgg cgtggcgcag tgccttctgg tgatacaacg accggccatt
    21061 ccagcacccg gcttagcgtt acgttctcct gaagtagcgg gtgattgggg cggacaacca
    21121 gcttcagcga ttcaagaaac agcagttcat aattaagccc gttcatcagt tcaggatctg
    21181 acatccgacc aatgccgata tcgatttccc cggtttttaa acccgccaga atcatagggt
    21241 tactcattgt cgcaacttgc aaggtcgtct ctttttgttg ctgatgaaac tgacctataa
    21301 ccgaaggtaa tatccctagt gccgcagtag gtagcgcacc aaccctgacg acatcattat
    21361 taagaccttc tttacgatga agcgactgtc cggcagtgtt gatggcgtca agaactctga
    21421 ctgcatgcgt taaaaattgt tcgccgggta aggtaagttg cgccccctga cgaccacgct
    21481 caaacaagcg agcgcccgtc agctgctcca gttcattcaa tgtcttagag agcgcaggtt
    21541 gactcaaatt aagggtttca gccgcgcgcc ccaaagttcc ttgttgtgcg acagctacga
    21601 atgtatgaag gtggcgcaaa cggatgcgct gactaaacag actatttttt tccataagcg
    21661 atgttaaaaa cgaagcggtg tcgctgacaa gtgaagttgt tttattatga taacttgatt
    21721 gcaaaatatt attaacaatt aaagcaacta tgttacagca aaatggataa tattgatgtt
    21781 ttcgcggcga gatcacagtt tgtaaattct tccagccgga gtgaatgcgg ttacctacac
    21841 tccagattac tgaccactgg aggcagacac tatggcgaac agcatcacgg cggatgagat
    21901 tcgggaacag ttttcgcagg caatgtcagc catgtaccag caagaagttc cgcagtacgg
    21961 cacgctgctg gaactggtag ctgatgtaaa tctggcggtg ctggaaaaca atcctcaagt
    22021 gcacgaaaaa atggtaaatg cagacgagct ggcgcgactg aatgttgaac gtcatggggc
    22081 gattcgcgtt gggactgcac aagagcttgc tactcttcgg cggatgtttg ccattatggg
    22141 gatgtacccg gtgagctatt acgatctctc gcaggcaggg gtgccggtac attcgacagc
    22201 atttcggccc attgatgatg cttctctggc gcgtaatccc ttccgcgttt ttacctcgtt
    22261 actccgtctt gagcttatcg agaacgaaat tttgcgccag aaagcggcgg agattctgcg
    22321 tcagcgcgat atcttcaccc cacgttgtcg acaactgtta gaggaatatg agcagcaggg
    22381 cggttttaac gaaacacagg cacaggagtt tgtgcaggaa gccctggaaa cgtttcgctg
    22441 gcaccagtca gcaacggtag atgaagaaac ctatcgcgcc ttgcacaacg aacatcggtt
    22501 gattgctgat gtggtctgtt ttcctggatg ccatatcaac catctgacgc cacgtacgct
    22561 ggatattgac cgggtgcagt cgatgatgcc tgaatgcgga attgaaccca aagttctgat
    22621 cgaagggccg ccgcgccgcg aggtgccgat tttactacgc cagaccagct ttaaagcact
    22681 ggaagagacg gtgttgtttg cggggcagaa acagggcaca cataccgcgc gctttggtga
    22741 aattgagcag cgtggtgtgg cattaacgcc gaaagggcga caactgtatg atgatcttct
    22801 gcggaacgct ggaaccgggc aggataatct cactcaccaa atgcatttac aggaaacctt
    22861 ccgcactttt cctgacagtg agtttttaat gcgtcagcaa gggctgggat ggttccggta
    22921 ccgtctgacg ccttcgggtg aggcgcatcg tcaggcgatt catcccggag acgatccaca
    22981 gcccttaata gaacgtggtt gggtagtggc gcaacccatt acctatgaag atttcttgcc
    23041 cgttagcgcg gcggggatct tccagtcaaa tctgggtaat gaaacgcagg catgcaatca
    23101 cggtgatgcc agtcgcgaag catttgagca ggcgttgggg gggccggttt tggatgagtt
    23161 ccagctttat caggaagcgg aagaacgcag taaacgtcgc tgtggtttgc tttaaattct
    23221 gaccatccgc ctttgcaaaa atttgcctga ttttacaaac gaatcaggct catcccatcg
    23281 acataaaaaa aatgccaatt tatgcatatt ctctgatttc aacaattgga ttattaataa
    23341 atattgtcta gagtgagcgg tcataaataa gcactttctt gccgctgaaa atgaccagcg
    23401 cgggaccatt cacaacacca gaaggactca ctttcaggta tggatcgtag acgatttatt
    23461 aaaggttcaa tggctatggc cgccgtgtgc ggtaccagcg gcattgcttc tcttttttct
    23521 caggcggcat tcgcggccga ttctgatatt gccgacgggc aaacccagcg ttttgacttc
    23581 tccattctac agtcaatggc gcacgactta gcgcaaacag catggcgtgg tgcaccacgt
    23641 ccgttacctg acactttggc gacaatgacg ccgcaggctt ataacagtat tcaatacgac
    23701 gccgaaaaat cgctctggca taacgttgag aaccgtcaac tggacgctca gtttttccat
    23761 atgggaatgg gattccgtcg ccgcgttcgt atgttttctg tagatccagc aacacatctg
    23821 gcgcgtgaaa ttcactttcg cccggagttg ttcaaataca acgatgcggg tgtggatacc
    23881 aaacaattag aagggcaaag cgatctcggt tttgccggtt ttcgcgtgtt taaagccccc
    23941 gaactggcgc gccgtgatgt agtatcattc ctcggcgcga gttatttccg cgccgttgat
    24001 gacacatatc aatacggttt gtcggcccgc ggcctggcga tcgacactta caccgacagc
    24061 aaagaagagt tccccgactt taccgccttc tggtttgata cggtaaaacc aggggcaacc
    24121 acctttaccg tttatgcgtt gctcgatagc gccagcatta ctggtgccta taagttcact
    24181 atccattgtg agaaaaatca ggtgattatg gatgtggaaa atcacctgta tgcgcgcaaa
    24241 gacattaaac agctgggcat tgcgccgatg accagtatgt tcaactgcgg tactaatgaa
    24301 cgtcggatgt gcgacaccat tcatccgcaa atccatgact ctgatcgtct gtccatgtgg
    24361 cggggcaacg gcgagtggat ttgccgtccg ctgaacaatc cgcaaaaatt gcagttcaat
    24421 gcttacaccg acaacaaccc gaaagggttt ggtttattgc aactggatcg tgatttctcc
    24481 cattatcagg acattatggg ctggtataac aaacgcccaa gtctgtgggt ggaaccgcgt
    24541 aacaagtggg gtaagggcac catcggcctg atggaaatcc caacaacggg cgaaacgctg
    24601 gataacattg tctgcttctg gcagccagaa aaagctgtaa aagcgggtga tgagtttgca
    24661 ttccagtatc gtctgtactg gagtgcgcaa ccgcctgttc attgcccatt agcgcgcgtt
    24721 atggcgacgc gtaccggcat gggcggtttc ccggaaggtt gggcgccagg tgaacactat
    24781 cccgaaaaat gggcgcgacg ttttgccgtc gatttcgttg gtggtgatct gaaagctgcc
    24841 gcgccaaaag gcattgagcc ggtgattacg ctttccagtg gggaagcgaa gcaaatcgaa
    24901 attctctata ttgaacccat tgatggttat cgtattcagt ttgactggta tccgacttcg
    24961 gactccactg atccggtcga tatgcggatg tatctgcgtt gtcagggcga cgctatcagt
    25021 gaaacatggc tgtatcagta tttcccgcca gcgccggata aacgtcagta tgttgatgac
    25081 cgcgtgatga gttaatcgtt ttttcttcgg catcttcttc gggaggtgcc gtctggttaa
    25141 acacgatccc gctcgcattt ttccctaagt taaatgagta atctgatggt gtgtatttca
    25201 gatacacctt gtcagccact aacagggagt gcgtatgttt ccagaatacc gagatttaat
    25261 atcccgtctg aaaaacgaaa atcctcgctt tatgtccttg ttcgataaac acaataaact
    25321 tgatcatgaa attgccagaa aggaaggttc cgacggtcga gggtacaatg cggaagtggt
    25381 ccgcatgaaa aaacaaaagc tacagttaaa agatgagatg ctcaaaatcc tgcagcaggt
    25441 gagcgtcaaa gaggtgtaaa ctttcctaag ccgccagcca ggcggctttt ttaacagctg
    25501 catggattga ctggagataa gatgactgaa acgataaaag taagcgaatc acttgaatta
    25561 catgctgttg cggaaagtca tgtcacacct ctttatcagt taatctgtaa aaataaaacc
    25621 tggttacagc agtcgctaaa ctggccgcag tttgttcaaa gtgaagagga cacgcgaaaa
    25681 acggtgcagg gtaatgtgat gttgcatcaa cgcggctatg ccaaaatgtt catgattttc
    25741 aaagaagatg aacttatcgg cgttatctct tttaatcgta ttgaaccact gaataaaacc
    25801 gctgaaatcg gctactggct ggacgaatct catcaggggc aggggatcat ttctcaggcg
    25861 ctgcaggcat tgattcatca ttacgctcag tctggtgaac ttagacgctt cgtgatcaaa
    25921 tgtcgggtgg acaatccgca aagcaaccag gtagcgttgc gcaatggttt tatccttgaa
    25981 ggttgcctta aacaggctga gttcctgaat gatgcctatg atgatgtgaa cttatacgcg
    26041 cgtattatcg attcacaata accctgccag cagcgttcgc gtaatgcgct cctcgccgtt
    26101 aatgactttt ggaccacgga catgaatcgt gtcgccatca aaggcttcaa cgacggcgtg
    26161 gtcagttaac tccacatggt tttcaattat caccgcacct gtaatacggc tctcaccttg
    26221 tatgacaacg tgctcgtcga gcagaatcgg tccgccacat accacggcat ttccgccaat
    26281 caagacatga tgttttaaca cacaattacc ttccacaatg gcatattccg ccacctgcga
    26341 actgtaatga atcgtcggaa tggcatcttc ttctatgcca gctttcacct gcgcgtgacc
    26401 gtagacttta gcgcaatcgc atagccagac gttgttctct tcattacctt caacgctggc
    26461 aaaatcaaaa acttcggcgc gatgttcaat aaaagcatac cggacgacgg catcgccata
    26521 aatttgtgcc tggtggacaa tacgcgaggc gctaaccctc gcgcgatcat aaatttgtag
    26581 caggagttga tggtcgggcg ttaagccttg tgcggcgacg atcatagaat gttgatcaat
    26641 taaggcgtga ccaaatattc tacattgacc gcaaactaat gagtcacgaa tggtgacact
    26701 gtcacttata tatgcaccct ggctgatttc actattatcg atccagacat tatccgttgc
    26761 gtaaacctct ccccacagca cgctggtgcc ggtgatacgc gtattcccgg aaatcaccgt
    26821 tccgccgaac gcaatagcat tctggtcgta aatccagcaa tttccttcct gtgctaacac
    26881 catttcgcga tcgatccagc cgccagcggt tcctgctatc acatcgttaa aatcgctaat
    26941 ggcgataatc tgccgtaata acacattttt ttagtgccat cttcctgata actaaaggct
    27001 cgctgttctt cacttagacg atatttgcgc atagcgtttt cccacaggtg acttactata
    27061 accgtagcaa attctgcggc tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa
    27121 atatacttta taaattaaac aaaatgagta agaagatgca gagcgataaa gtgctcaatt
    27181 tgccggcagg ctactttggt attgtgttgg ggacgatagg gatgggattt gcctggcgct
    27241 atgccagcca ggtttggcag gtcagccact ggttggggga tgggctggtg attctggcga
    27301 tgatcatctg gggattattg actggcgcat ttattacccg actcatacgc tttccgcata
    27361 gcgtgctggc agaagtgcgc catccagtgc tgagcagttt tgtgagtttg tttccggcaa
    27421 cgacgatgct ggtggcgatt ggttttgttc cgtggtttcg cccactggcg gtgtgcctgt
    27481 tcagttttgg tgtcgtggtt cagttggctt atgccgcctg gcaaactgcg ggattatggc
    27541 gcggatctca ccctgaagaa gctaccacgc ctggactgta tctgccgaca gttgccaaca
    27601 actttatcag cgcaatggcc tgtggtgcgt tggactacac cgacgccggt ctggtgtttt
    27661 taggcgcagg cgttttctca tggctaagcc tggaaccggt gatcttgcag cgtctgcgca
    27721 gttcgggaga attacccacg acactgcgga catcactcgg cattcagctc gctcctgcgc
    27781 tggtggcctg tagtgcctgg ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa
    27841 tgcttttcgg ttatggactg ctgcaactgc tgtttatgct acgtctgatg ccatggtatc
    27901 tctcccagcc atttaatgct tcattctgga gtttctcgtt cggtgtatct gcactggcaa
    27961 ccaccggttt gcatctgggg agtggcagcg ataatggatt tttccatacg ctggcggtgc
    28021 cgctgtttat ctttaccaat tttattattg caatactgct catccgtact tttgcgcttc
    28081 tgatgcaggg aaaattgtta gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca
    28141 aagaatgatc attcgtgatg aaaactattt tactgataaa tatgaattaa cccacacaca
    28201 ctctgaagtg ctggaagcgg tgaaagtggt taaaccgggt aaaacgctgg atctgggctg
    28261 tggcaatggt cgtaacagtc tttacctggc agccaatggt tatgatgttg acgcatggga
    28321 taaaaatgcc atgagtatcg ccaacgtcga gcgcattaag tccattgaaa atctggataa
    28381 tttacacacc cgggtcgttg atctgaataa cctcacattt gatggacagt acgattttat
    28441 tctttctact gtggtgctga tgttccttga ggctaaaacc atcccagggc tgattgccaa
    28501 tatgcaacgt tgcactaaac ctggcggtta caacctgatt gtggcggcga tggataccgc
    28561 tgattatcca tgtaccgtcg gcttcccgtt tgccttcaaa gagggggaat tacgtcgata
    28621 ttacgaaggc tgggagatgg tgaaatacaa tgaagacgtc ggcgaactgc accgcaccga
    28681 cgccaacggt aatcgtatta aactgcgttt cgccacgatg ctggcacgta aaaaatgatc
    28741 cggtaataac aaagcgcgtg aaaattcccc acgctgagat ggtttactgt tcttcttttc
    28801 ggtaagcata ttttttatcg aagggatgtg aaattaatca cagtagtcga agttttttag
    28861 ccgcttaact tactgaaatt taactactga tgattgactt agcccctttt tcggcattga
    28921 ctatgtcgtc tgaaaagggg ctaaaaaatt tattttcacc aacacttttt ttgccacaac
    28981 acgaagcggc gctttttgct ataacttaga aagtaatata atcatctcag gaaactattc
    29041 atgcgtacca catcatttgc gaaagttgca gctttatgcg gcttgttggc tctgtctggt
    29101 tgtgcttcta aaatcaccca gccagataaa tattctggtt ttttaaacaa ttactctgat
    29161 ttaaaagaaa caacctcggc aacaggtaaa cctgttttac gttgggtaga tccgagtttt
    29221 gatcaaagca aatatgacag catcgtctgg aacccaatca cttattatcc ggtaccgaaa
    29281 ccttctaccc aggtagggca gaaagttctg gataaaattt tgaactatac caacaccgaa
    29341 atgaaagaag cgatagcgca gcgtaaacca ctggttacca ccgctgggcc gcgtagtctg
    29401 attttccgtg gggccattac cggtgtagat accagtaaag aagggctgca attctatgaa
    29461 gtggttcctg ttgcattagt ggttgcgggg acgcaaatgg ctacaggcca ccgtaccatg
    29521 gatactcgct tgtattttga aggtgagctg attgatgcag cgactaataa accggttatc
    29581 aaggtcgttc gtcagggcga aggtaaagac ctgaataacg aaagtacgcc aatggctttc
    29641 gaaaatatta aacaagttat tgatgacatg gcgaccgatg ccaccatgtt tgacgttaac
    29701 aaaaagtagt tccagacgcg ccatcgttag atggcgcttt ttattatcgg tttctgttag
    29761 cgattaaatt caacaccacg taacaaacgc ctccggcaat taatccccaa aacgcagaac
    29821 caatcccgaa cagcgtcaat ccacttgccg ttaccagaaa tgccaccacc gccgcgtcgc
    29881 gctcacgctc attatgcagc gcctgataca aactgccgcc aatggtactc aacagcgcaa
    29941 gacctgccag catctggatc caacttacgg gcagggcagc catcatcccg gtaatggcac
    30001 taccaaacag acctgcgatc aaataaaaaa taccagcaac ggcagcggcc agccaacgtt
    30061 gatctttatc cggatgcgct tccgggcttt ggcaaatagc cgcggtgatt gccgcaatac
    30121 cgacggaata aacgccgaaa ggggaaaaaa caagcgccag caatccagta aacacaatca
    30181 acggcgaaac aggagccgaa tacccggctg ctttcattgc tgcgataccc ggtgcgtttt
    30241 gcgatgccat cgtcaccaga aaaaggggga gtgcaacgct cagactgtga gcaaacgaaa
    30301 aatcaggggg aatataagtg gggagaacgg gtttaaagac aacatcagtt gtgacaacgt
    30361 caccttgcgc gataacgatc acgatcccaa taatcatcgc ggcaattacc gcatagcgcg
    30421 gcgcaacggc cctggttgcc agccatacca gcaacatact gccacacaac gtaaattgac
    30481 catccagact ggcaaacgcc tgtaaaccaa aacgtaataa aatcccggca agcattgccg
    30541 ccgcaagcga gtgcggaata atgcgcatca gacgagcaaa gagtcccgtt atgccgcaga
    30601 ggactattaa cacgttggtg acaataaaaa cgccgatggc ttcgttaagt gttagtccct
    30661 gtaatccggt gaccaacaaa gccgcgccag gcgttgacca tgcggtgaga acaggtacgc
    30721 gataccataa tgtcagagtc agcgtactga cgcccattgc cagccccagc gccgtcatcc
    30781 agccagagat ttgtgcagtg gtggctccgg cgacaatcgc tgcttgccag attattgccg
    30841 ctgaactggc gtagccaatt aataccgcca gaaaccccgc cagtagcgtg ggtggaggaa
    30901 tagaaaacag acgcatagtc acctcgtacg ttataacgta cggctaacgt agcatttgtc
    30961 cgttataacg cacaagtgat aaacttccgt tttgccggag gagtcgcatg gaaaatctcg
    31021 ctcgcttttt atccaccaca cttaaacaac tacgtcagca gcgcggctgg agtctttcgc
    31081 gactggcaga agcgacgggc gtttctaaag cgatgttagg gcagattgag cgtaatgaat
    31141 ccagcccgac ggtcgcgaca ttatggaaaa ttgccaccgg gttgaacgtg ccattttcaa
    31201 catttatttc tccgccgcag tccgctacgc cttcagttta tgatccacaa cggcaggcga
    31261 tggtgatcac ttcgctgttt ccttatgatc cacaactctg ctttgaacac ttctcgattc
    31321 agatggcacc cggtgcaata agcgaatcga cgccccatga aaagggggta atcgaacatg
    31381 tcttcgtcat tgatggacaa cttgatctgt gtgttgatgg tgaatggcag agccttaatt
    31441 gtggggaagg cgttcgattt gctgcagacg tcacgcatgt ctatcgtaat ggtggggagc
    31501 aaaccgtaca ttttcattcc ctcatccatt acccgcgcag ttaagcggga aaactatttc
    31561 gcaacgtcgc gcttgtggct aaaatagccg ccatttttca gctactggat aagaaagtga
    31621 ccgtatcttc tcatcgtctt gaactgttaa gcccggcacg cgatgccgcc attgcccgcg
    31681 aagctatttt gcacggtgcc gatgctgttt atatcggcgg ccctggtttt ggtgcccgtc
    31741 ataatgccag taatagcctg aaagatattg ccgagttggt gccgtttgcc catcgttatg
    31801 gtgcaaaaat tttcgtcacg cttaacacca ttttgcatga tgatgagctg gaacccgcgc
    31861 aacggctgat tactgacctc taccagaccg gtgtcgatgc gctgattgtt caggatatgg
    31921 ggattctgga acttgatatt ccgccgattg aactgcacgc cagtacgcag tgcgatattc
    31981 gtacagtaga aaaagcgaag ttcctctctg atgttggctt cacgcagatt gtgctggcgc
    32041 gagagctgaa tcttgagcag atccgggcga ttcaccaggc tacggacgcg accattgaat
    32101 tctttattca tggcgcattg tgcgtggcct attcgggtca gtgctacatt tctcatgcgc
    32161 aaacagggcg tagcgccaac cgtggcgatt gctcgcaggc gtgccgtttg ccatacacat
    32221 tgaaagacga tcaggggcgt gtggtttcct atgaaaaaca tctgctgtcg atgaaagata
    32281 acgatcagac tgccaacctc ggcgcgctga ttgatgctgg cgtacgctcc ttcaagattg
    32341 aagggcgtta caaagatatg agctacgtga agaatatcac cgcccattat cgtcagatgc
    32401 tggatgccat tattgaagaa cgtggcgatc tggcgcgcgc ttcatcaggt cgtactgaac
    32461 atttctttgt tccatcgacg gaaaagactt tccaccgtgg tagcacagat tattttgtga
    32521 atgcccgtaa aggcgatatt ggcgcgttcg attcgccgaa atttatcggc ctgccggtgg
    32581 gcgaagtatt gaaagtagcg aaagatcatc tcgatgttgc cgttaccgag ccactggcaa
    32641 atggcgatgg cctgaacgtg atgattaaac gtgaagtcgt cggttttcgt gctaatacgg
    32701 tagagaaaac cggagaaaat cagtaccgcg tctggcccaa tgaaatgcca gcagatttgc
    32761 acaaaattcg cccgcatcac ccactaaacc gaaatcttga tcataactgg cagcaggcac
    32821 tgacaaaaac ctccagcgaa cgccgggtgg cggtagacat tgaactgggc ggctggcagg
    32881 aacaactgat tctgaccctg accagtgaag agggtgtcag catcacacat acgctggacg
    32941 ggcagttcga cgaagccaat aacgccgaaa aagcaatgaa caatctgaag gatggtctgg
    33001 caaaactggg gcaaaccatc tattacgccc gcaatgtgca aattaatttg ccgggggcgc
    33061 tgtttgtacc aaacagtctg ttaaaccagt tccgccgtga agccgccgat atgcttgatg
    33121 ctgcgcgtct tgccagttac cagcgcggca gccgtaaacc ggttgctgat cctgcgccgg
    33181 tttatccgca aacgcatctg agtttcctcg cgaacgtata caaccagaaa gcgcgtgaat
    33241 tttatcatcg ctatggtgtg caactgattg acgcggcgta tgaggcacat gaagagaagg
    33301 gcgaagtccc ggtgatgatc accaagcatt gtctgcgctt tgccttcaat ctgtgcccga
    33361 aacaggcgaa aggcaatatc aaaagctgga aggcgacgcc aatgcaactg gttaacggcg
    33421 atgaagtatt aacgctaaag tttgattgcc gcccatgcga gatgcacgtc attggcaaaa
    33481 tcaaaaatca catactgaaa atgccgttac cgggaagcgt agtggcatcc gtaagtccgg
    33541 atgatctgct gaaaacattg ccgaagcgaa aagggtaaaa cgccagtttt cgggttactc
    33601 accactcatc aaatctgcat gatattgcct gccgggtaag gcgtcatgcc gcatccggca
    33661 acaaatgact caacgctgtc ccgcttctgg tttgcgcgat ttttgccagt aaaaatgctc
    33721 gcgcaaacct tccgccgact cttccgccac agcacgcaat tcatcgctgt ccgcttcatg
    33781 acgcagctga tgatccacat tctttaccca cataaattca tgtcctttgt gccctgccat
    33841 gagttgtcct gagaactgta accaggtgaa tcatttgata ccaacgaggg ttttagtgaa
    33901 aggaggtgaa atcacctgta agatactgaa ataaaacagg tgatgtctgg atgagcgctt
    33961 aaagtctatt tgatatcgat tgtgcaataa ttaatcgttt attctaaaat cggcaaaact
    34021 tttgatactg cgatctcaac tttttaaagc gtttaaaact tcattaaatc tatatggcaa
    34081 ctaacatacg ggccagcctc gccaaactaa cccgctttag attctaccgg atttgattgc
    34141 ctacttgtct cggttttttc caaaatctct ctgacgcaat cgtctggcat atctacaatc
    34201 atcaacgcag ccaggcgctt cctgccttct agttgtaata aatcctggtt ttctttattc
    34261 agcagtgtca gaaccgtctc tacacctttg cgagcaagga gattggttaa ctgttctcgt
    34321 tcgtctttcg taaggaatga aagcatctgt gtaagctgat ctcccttatc accagaacaa
    34381 ccaaaattac cataaatacc aatatcgttt ggtaattcac ccttgccctc tttaagccac
    34441 tcaagactaa cgccttcact ttcagaaatt cgtataagga tactttctct gggggctggt
    34501 tgtgtttgtc ctcccttgaa atagctgttt aatgtgttga tattgattcc ccacgctctg
    34561 gcagctgcac ttcttgaagg gtagcgcttt gtaagcattc caatacgttc aataacaggt
    34621 tcttttccct gcgacataaa gatacctctt ggtgcttttc taaggaaagg tttctttcta
    34681 tttatccaat tgaaaaatga ggataaaagc aaaaattagc caaaacacca aaaatacctt
    34741 gatttggtat ttttggtgat gcattattta cccgtacaga tatccgcacg aatatccgcg
    34801 cgggtaactt tttagaatag aggaagtatg agtagaagag aagtttcaga tcacgattgg
    34861 ccgcctgaaa tcatcaaggc gcgcttacac atggctggct tatcactgcg ctctttatct
    34921 ttaaaggctg gttatagcag ggattcgctg aaaagcgtat tacgtactcc ttgccgaccg
    34981 tatcagcaaa taattgctga tgctcttggg gtatcacctg aagaaatctg gcccagtcga
    35041 taccaggtta agagctatat gagaaaggcg tcatgatatg tatgtcattg caaaagaact
    35101 gattggcgcg cccggaatgc ctgctacaac aaaaggtatt cgccaggcat tacaacgtta
    35161 cgtacaaggg aaaagctgtt gttcccgtcg tcgctcaggc tctaaagcaa ctgaatacag
    35221 catcgactgt ttacctgaag tgacgcagca ggcattacgt gaacgctacg ccctgcaact
    35281 gatgacgcaa aaagccgatg aatcaccggc tccggtggtg acaaaggcca gacgctcacc
    35341 tgccgtggtt gatgcagtgg aggcatatcg cggatcaccc caactgatgg tcgaacgcct
    35401 caatgccctg actgaaaacc aacgtaaagt ggcagaggca cgaatagcga tcgtcagcga
    35461 ggtgctgaaa atcgcgcaac aacccggctt tagttgcgct aaggctatcc ggttcctcgt
    35521 taacagcctg gcacgatcac aactggacga gcgcattgtg gcaatggttg agacggcgaa
    35581 cgccaaaaag ggaaacagcc gcgcgttgag tgaaatcacg ctgaaacgct ggattgcggc
    35641 ctttaacaag gcacagaacg ccgctgaacg cctgctttta ctggcaccgg gtaaacgcca
    35701 ggaaataaaa gccgaagata ttaactggct gcccgaattt ctggcgcagt atcgccagtc
    35761 aaacggccga ccaatgaccg aggcttacga ggattttgtt gctgaatggc agcaccggca
    35821 cgctgatgag ccttatatgc tcgatatcat gccctcttat gacaccattc gccgcgcaat
    35881 gaagaaactg ccggaagtgg tgaaacaaaa aggccgggta actggcagtg aataccgtca
    35941 gcttgaggga ttcacgcgcc gcgactggtc cagaatgccg gtgaattatg tctggattgg
    36001 tgacggtcac ggcatgaagc tgaaatgcgc gcacccggtt cacgggcggc cattcgcacc
    36061 ggaagtgacc tttgttatcg acggtggcac gcgctttgtg gtgggctgga gccttgacct
    36121 ggctgaaaat gttttcgccg tagccggtgc catacagcac ggtattcgcc atcatggcaa
    36181 accgtttctg tattactcgg ataatggctc cggggaaacc gccgacgtcc tggataagga
    36241 gattgtgggg atactgccac gactggggat taatcacccg acagggattg ccggtaatcc
    36301 gcagggacgg ggcattatcg aacggcttaa ccgcacatta ccgatgcgca tagcccgtaa
    36361 ataccgcacc tatttcggga aaggagcaga tcgcgagacg ttacgtaaaa ccaaccgcga
    36421 tttacgctcg gcattcagtg ccctgcaaca gggcaaacgg ctgaacgccc ggcagcagtc
    36481 agcgatgcgt gatttaccgt cctggtctga actgattgat gccattcgtg acggtgttga
    36541 gtggtacaac aaccggccgc acgatgaatt acccatgaag ccgaacggta aacattacag
    36601 cccggcggag ttcagaaaaa aacgcctggc ggaagaggac acggaaattg aatggctgtc
    36661 cgatgttgaa ctgcgggata tgttccggcc gatggtggag cgccctgtaa gacgctgtga
    36721 aatacgctgg ctgaataata tttactacgc gcccgagctg cgtgacgagc atggccgtaa
    36781 ggtacttatc agctatgaca ttcatgatgc cgaacgaatt accgtgcgcc gcccggatgg
    36841 cagcgtgatt tgcgaggcgg tatgggacgg caataaacgc gaagccttcc ctgtcagcgc
    36901 ggaatactac aaacagcagc agcgccttaa aggcatgcgt aaacgcgcag aggaaaaaat
    36961 ccgtgatgcc gaggatgagg ttgtcaacgt gctggagcac aagccgcagg agccctggct
    37021 ggaaaacata taccgccctg tgggtaatac ggtgaccgtt cagcaaccgg tcgctgacga
    37081 tgaacctgat gaagaatacg agcgtaattt ccagcgggga ttgcaactgc tcgaagcgaa
    37141 attaaaagaa agtgatccgc tggcctgaaa taaaaaaata acccgagggg tgactcaggt
    37201 tatttgaggt ttcagtctcg gagaatgctg tctcaagcga ggtgaataat atgaccgata
    37261 ttaacgatgt aatcaagacc attgatgaac ttattgatgg cggcgtactg acgcagtatg
    37321 ccatcgccag agaggcggga atttccgacg gcacattatc ggctttccgt aagggaaaat
    37381 ataaaggcga taacgctgct gtggctgctt ccctgcgctc ctggtatgag aactggaata
    37441 aacaaagcgc actgccggaa ccgccgcagt ttgtggaaac tcagacagtc caggagctgc
    37501 gcgcactgtt tcaggcggtt cgcctgatgg gctgtattaa cgttattgtg ggcgtaccgg
    37561 gtgtgggtaa aacggccact gcccgtaatt actgccagga gcaaccaaac acctggatga
    37621 tcaccctgtc acccgcgcac tccagcgtca cggagtgtct gctggagctg gccgatgcgc
    37681 tggggattga ttacacccgc gcgaacaaag gggcattatc ccgcgccatc cgccgtcgcc
    37741 tgatgggaac gcgtggactg gtgattgtgg atgaggcgga tcatcttggt attgacggtc
    37801 tggagcaact ccgggcaatt caggacgcca cggggatcgg gatggtgctt attggtaacc
    37861 cgcgcggatt gtttaaaggt ggacgccgcg cctttgatga tttatcgcgc ctgttcagcc
    37921 gtcttgcccg tacaaaacaa cttcgcaagg ccaaaaaggc ggatgtgctg gccattgcca
    37981 tggcgtgggg gatcagtggt gaggccgagc tggccgtcat gcaggctatc gctgaaaagc
    38041 cgggagcgtt acgcgttctg acacatacgc ttaaccaggc gtggctcacc gccagcggtg
    38101 aaggcgcggc gctgacagaa aaacatatta atgcggcctt taaagaggtt tataccaacc
    38161 ctgaattact ctcacaggtg tgattatggc ggtatttaat attcctgata tttacggacg
    38221 cttttacctg gttaatttcg ataacgtgaa ggtgatttca ctggccgaaa ataaagaatg
    38281 tggcgattta ctttttgaat ttaatgaccg cacacgaatg gtgatatctg ccggacttga
    38341 tcgcgaaggt gcgacagaag tttacagcgg aatatgccgt tctgttggtg cgaaacaagt
    38401 cagctaaatg aggtgttata tgaatatgca atcctgcggt aacaaaatga atttattcga
    38461 ctccctgaac agcgcgcgcc gtctgaccga acttgccggt gcggtactgg aacgcagtaa
    38521 gcgctatccg caacgttttg cactgaaaac cacgccgccg gtaggcaacg tgcagggaac
    38581 cggtgaaatt gaaatcacca tacagaccaa cggcctgcgc cgccgtgtga aggccacccg
    38641 catcagcggc tgcacggttt actgggaggt gtgaggtgaa aaaaaatctc attgcatggg
    38701 cgtggtcgag tggtcttatt gagtttggtt acgtcctgcc ggaaggtgca ttgccgatag
    38761 ttgccggaaa gcctgccacg gtacggcatg tgattgaggt tatggcgcgt catggacgtg
    38821 atgaacagga gcagttactg gttccgggga taccggaagc ggtgacggag gaagaagcct
    38881 ttaatgccat gattcggttc tgccgtgagg ttagacgccg ggtcagttat ccaaacagaa
    38941 cgaggaccag aggatgagta aagtcgtacg cattattttc gaatacaagg aagatgttat
    39001 ctgcaaaaac tctgatggca gcacgcacag aggggtaagt ctggatatac gttcaaccgg
    39061 aataaagaag aaagataatg gacccgccat gatttttggg gtggttatgc tggcagaaag
    39121 cagaaacttc gcagagtttg tggcaatgaa agccagtgca ttcatgaaag acagaggcat
    39181 ggcttccggg gttattaacg gtaatgaatt taatcaacag gggtaattcc atgagcaaag
    39241 tacgcgttat ttttgaattt aatcatgttt cgcatgacga aaagttggca ggcaatgact
    39301 gtgttgaagt gcatgaaaag attggagtgg atgtgaaaac agaacgtgat acggataaca
    39361 ggccgacgtc actctgtgac gtttatgcaa gtattctcca gtatcacagt cctgcaatta
    39421 ttcagtttct ctcagcggaa tttcaggcat ctgcacagtc ttttggagcg gatgccatca
    39481 ttaaacgcca ccgcgtgcat aaagcatcag gcacactgca ataaggaaaa acaaaatggc
    39541 taaacgcgtt acaaaattaa aggccgcagc cgaggcggca ccgcagaccc gggaagaagt
    39601 cagccgtgat atccgcaccc tgggagatat tcagcgagag gcgctgcgcc tggaaacggc
    39661 gatgaatgac gaagtggcag aaatcaccgc ccgttatacg ccgcagattg aaaatcttaa
    39721 aaagcaaatc aaagtgcttt ttaaagggat tcaggactgg tgcaaaacca accgtgatga
    39781 gctgacgaac ggcggcaaaa ccaaaacagc caatctgacc accggaacgg tgtcatggcg
    39841 gctgggaacg ccatcatgca gcgtcagccg tgatgtggaa ggtgtgattg aaatgctgcg
    39901 ccgtatgggg cttgagcgtt tcatccgcac gaaagaggaa gtgaacaagg aagccgtcct
    39961 ggcggagccg gatgctgtga aaggcattgc cggtatcaag gtgaataaag gcgctgaaag
    40021 tttttatgtc gagccttttg aacaggacgc cggactgaat aaataacacc acattaatca
    40081 attaaatatc acttcatttt aattatggcg ctcgcgtcag gggactgctt gcgcctgaaa
    40141 acagaaaata agggttaaga aatatgaaat acgtttatat cgtcattaat aatgtggtgg
    40201 ggaaataaat catgattgat gcaaaagtgc ttgaaggggt taaaaactgg ctgtgtattt
    40261 atggccgcct gacctgcggt gtactggctg aaaaaatgaa tatgccgcca tcctccatgg
    40321 tttattttct gcgtgatgcg gttgatgcag gcgtgctgac ggaatgtaac ggtttttatg
    40381 atattccgcg cccccgcccg gtgcagccgg ttcgtcgcaa atgcagccag gaatctgcgg
    40441 ctgatgatgt tcagtggtgc ggcttcagaa aatccctgcc atggattgag gggcatgata
    40501 ttccgtcgat ggcgtgggaa tttgctcagg gcgttctgac ctgtgaaacc gtttatgtgg
    40561 tggctgaagt tgatgagcag gccatgaaag aaggcgtgcc ccagtttgtg atggcgtata
    40621 tcgacatacg cctgggcgtc attatctgcg gtttaagcgg ctggaatatc actgaacatg
    40681 ttctgcgcta cctgattgtt gaccggacag cagcgcctgc cgggatatct gcggaggtgg
    40741 cgtaatgttc tttaaaacat caaacccttc cgcgctggct gcgtggcaaa aataccagca
    40801 ggactgccag aaagttaagg atgaggcaaa acgccttgag gccgtgctga atgttgcgtg
    40861 ccggtcggta tttgtatccg gtatcagtgg attttacttt aagggactgc gttttacgga
    40921 ggacaaatat ccttttcatc gcgacttatg gcgaaaaccg actgcgtcga atggctggag
    40981 ctgcacaccg cgcacatcac gtatccctaa aaccctgcgt gttgcctctg atgaactgaa
    41041 cagtctgtgg cgtgaatatt cgcccgtcac gtatgccaga accgatgcgc tgttgttctg
    41101 gttgggtatt gatttctcgg caatcctgtt tggccctgtg aagtggttct gcgttgacga
    41161 tgtgatttac cttcagtgcg gcgtaaaacc cgcaaaacag aaaatgaccg aaatactgtc
    41221 tgatgagttt tatgctgctg aaaagcgagt cagggggtga tgcatgatga aattacaacc
    41281 catgggacta aaaggtcgcg cccccgctca tgtccgtccg tggacacctg aagaagatgc
    41341 gctgctgatt gcgctttatt catccacccc ggttaaggat attgctgcca gaataaaaag
    41401 aactgtctgg gctgtatata accggactgg tgtattgcgc agttcatacc cggagttact
    41461 gaaatataaa cacccaagat ttacacctga tgaagataag tttattcgaa aaaatgccag
    41521 aacaatgacc tgccagcaaa tgggagaata tctcggacgt aataaagatt ctgtcagatg
    41581 tcgggcagga atgattggtg ctggattaac aaagtgcgga gagttacgcc ccggcacgcg
    41641 catatctgat gatgatgtac gtcttatacg tgcgctgcgt gattccgatt acccacgccg
    41701 tctgtcattc cgggaaattg gcgaaaagtt tggaatatct gaacattctg ctcacgcagt
    41761 ttattaccgt cgccggactg ccgaggacgc tgtattacgg gagttaacgc catgataacg
    41821 accttatttg ttgaatcaga tgaacctctt gtgtgtgccg ccggaatgcc gctctgtggc
    41881 ggaacgctga ccggcgttta tttcggggat ttgcgcggtt atccctggca ttcactggat
    41941 gacgcatttc cgcctgatat ggaggctgtc gtgctgattg ttcagtatgg tgaccaacag
    42001 gaactgcgca tcggccatat ggggtatgaa ggctttttta ttgatgaaga aaccggagcc
    42061 tgccttgaag atgaggacgg acaggtgacg cactggtgcc atctttcggc cttgccggaa
    42121 ttacagggga tacataatgg atgagcaaat tgttgaatgt cccacctgtg gaaatgagga
    42181 cccggagtat ctgaaagagt gcccacattg tggtgagata aaatgtaacc actgcgatat
    42241 gggcgacgac actgcatgta taaattgtga ggatgaataa tatggaaaaa gaaatcaaat
    42301 ttgcgcctaa atctatcgac gaagaactgg ctaaaatcgg catgcttgaa cgtatgcggg
    42361 acattatcga atatgcgata aaagaaaatc ttgcagccag agaagccctt ttgataatgg
    42421 agcgggagat taacctgatt aaggatgctg tatctctgga taataaaata gcccgcgagg
    42481 aatacgttcg ccgtaggctc ggtgttgatg gttcagcaat tcttacatct gaacattatg
    42541 caaaaatttt taatcttttt cagcggtaat ccccatacag gcccggagat tgtcgaaatg
    42601 ccagtcggta ttaatatatg agccatcctg gatatcttct gcattttccg gggtagagtt
    42661 tttttgagcg agattgataa tatgtattgc ctcctcacgg gaaatcagcc ccttatcgaa
    42721 cagggttacc ataagatttc cagccattgt gaatgcggca acatcaagtg cattttttat
    42781 atctgacata gatttttcct tttatgttaa ttgacatggc ggtgcggtaa caccgcccat
    42841 tttttatgga gccacgataa tgaatcgcgc ttccctgatt accttaatac atgtcgcaaa
    42901 acgcgatcta cagcttgacc atgataccta tacatccgcg ctgctggcag ccaccggcaa
    42961 atccagttgc cgtgatatgt cacccggtga gttatcccgc gtgctggatg ttttcaaaaa
    43021 acgcggtttt aaagtgcgtc agaagccggt taaccgggcc ttaaaaccgg gtacggtgac
    43081 cgccaaaatt cgcgccatct ggaaagtgat gcaccggcag ggatttatct ctgatggcgg
    43141 ggaaacggcc cttaaccgct gggtgaaatc gcagacggcc gcgcagaacg acggcgaagg
    43201 tgtggctaac tggcagtggc tggagcaaca acccgcgctg gcctctgatg tgctggagcg
    43261 tctcaaacgc tggcaccgcc gcaaaatgct ggcagccctg gatatgtcgg aacgtgcagt
    43321 gatgagttat gagcgtgttt gcaggctgta tgaaaaatca ttcccccgtt aaccccaaat
    43381 cccgccacag cgcgggattt ttattttaaa cttgccctgt gtaatgatga ccggaggcgg
    43441 gtatggctga aacacagatg agcatgtttg gtgacagcga acagctgcac gcgctgattg
    43501 accgtctgga tgatattcct gatgatgagc tgaaaaagaa ctggcccggc acgctgcggg
    43561 acctggttga tgtgatcagt gctgaactgc accgtcaggg gattgaacct gctcaggcgg
    43621 gaatgcttgc ccgtaaggtt gcagccgcac aggccgggta catgggcggg cgtggttatt
    43681 atctcccggt cggtgaatcc cttttcaccg aactgcgcaa taatgaaatt tttttccgct
    43741 ggagccaggg cgaaaaaatt gaaaccctgc gccgccacta ccagatgtca gagacgcaga
    43801 tttactccgt tatccgcaac cagcgtcgcc tgcatcagga acgcacgcag ccacggcttt
    43861 tctgatattc cgcaaatcct gcccactttt tcccacggtt acgctgactc agagaacacc
    43921 atgagacagc gtaacaatgc caaaactccc cgcacccctg cgtaaaaagc tgattgccct
    43981 tgttctggcc ggagccggga cgttcaccat tgccacgcac tacaccggct actgggaagg
    44041 gaaagaaaac accacgtaca ttgatcccac cggcaccccc accatctgtt acggccatac
    44101 cggcccggat gtgaaaccgg ggatgacaaa aactgatgaa gagtgtctgg agctgctgga
    44161 aaaggacatg aaatgggcct ttgcggctat cgatcgtcat gttcaggtgc cgcttacccg
    44221 tggtcagacg gtggcgctgg cctcctggat tttctgggcc ggtgaaacga actttcgcaa
    44281 ctccacgctc ctgcgcctta tcaatgccgg gcagatgccc gcgtcctgta agcagtatat
    44341 ccgctggatt tattcaaagg gggtaaaact ccccggtctt gaggcccgcc gttcggcgga
    44401 tgaatggtta tgtcgttacg atttgccgaa agtctgaacc gcttctggcg accgctcatg
    44461 attgcgctgc tgtgtgccgt tgtgctgctg cggggtgtgc tgtgtctgcg gtgaacaatc
    44521 ccttaccggc cctgattgcc ggaggctgta tggcggcgct gggggtgatg agcgtgctgg
    44581 ctgccgtggt atggggaatg caccagaaga cgcagcgcct tgaggacaat aatcatgtgc
    44641 ttgtgcgtga acgggacgaa gcccgtctgg tgctggcaaa ccagcaacgc accctgcaac
    44701 tcatttcaca aatcagcaag gcggccacga atgaaaaaca gcagaacatg cagcacagcg
    44761 aggaacagca gagcattgtc cgccggtcac tggcaacggt gcctgctgcc gctgtgcctg
    44821 tgcctgatga tgtggctgac cgggtgcggc gggccgtctg tgaaattcgt ccctgtgccg
    44881 ccggtgccga tccccgctga atggctggct gactgcctgg tccctcccgc gccgcagccg
    44941 ttcacgtttg gtgcatcggt cacttacaac ctgcaactgc tggcggtgat taaaaactgc
    45001 aacgtggaca aggccagcat ccgccgtctg gagacacagc gacagcatga atttactgat
    45061 atggccggaa cgactgctgt tccggcagga aagacgaagt aaggaaaaag gtatggatga
    45121 ttcagattgt gctcaggcag tcatggagcg tgcaacagaa cgcgcccttt gtgaccggct
    45181 gacacgtaag cgccgggtgg cggagacacc gggcaggcgt gtatgcgcgg actgcggggg
    45241 tgacattccg gctgcccgtc tggttgccgt gccggatgcc atccgttgtg tgaactgcca
    45301 gaacatcatg gaggccggtc atgtgggtca gcatcgttaa agattatgtc gtgccgatcc
    45361 tgtcggcaac ggccacagcc ggggggatct tcatggcgct gatgcgcaaa acctttgtcc
    45421 cccgcgaggc ctttgaaaaa ctctccgacc gggttgagcg ggtggagagc cggatttccg
    45481 gcctgcccac gcagcaggag gtgaacagac tcaatatcga aatcaccacc ctgcgcggtg
    45541 agcttaaggc caccaatgcc accctgaaat ccgtctccca tcagaacgaa ctgctgctgg
    45601 agcaggctgt aaggaaaaaa cacaatgagt gatttcatta ctgaagatca gcgtcttgtc
    45661 attctgcggt cgctggcgga ttacaacggc gaactgggcg aatccgtgct tcaggactgc
    45721 ctggatgatt acggccaccg cgtgtcccgc gacaccgttc acacccacat tgcctggctt
    45781 gctgagcagg gactggtgcg taagcgcacg cttgttaacg gttatttcat cgctgaactg
    45841 accggccggg gtcaggatgt ggcggaaggt cgtgccaccg ttccgggtgt gaagaaacca
    45901 cgcgcaaggg ggtaacgatg gataagccga cgcgtggccg tgtacgcaag gtggatttgc
    45961 tccccgacag catccgtaag ccgctgctgg aaatgctgcg tgaaaaacgc ctgacgcagg
    46021 tccagatccg cgaggaaatc aaccgtctga tccgtgaggc aggcctgccg gaagagcagc
    46081 agctttcacc ggcggccatc agccgggaag cctcccgtaa tgagctgatt gcccgtaatc
    46141 tgcgtgattt acgcgagcag acaaaagcca tgatggccga actgggggat aagccgaccg
    46201 gtgaaaccac ggcactgatt ctggagatgt cccgtgccct gatgtaccgc cgtctgcgtg
    46261 ccgccaccga atccctgaac agcgacagcg atgtggatat gcgccttatt aaggacatcc
    46321 tcctgtcggc acagcgtgcg gaaagtgccg ccgagcgcag cattaagcgt gaaaaagaaa
    46381 tccgggccgc atttgctgaa gagatggcga acgccgtcac cgacgagttg cgcggcgtgg
    46441 atggcatgag cgagcagata gaattccgta tcaggcagat gctgcttggt aaggcgtagg
    46501 aggtgaatcg tggtaacaga taaccgctca ctgatgagca gtgctcatca gaaggcattc
    46561 agtcttgcaa cacagttacc tcctgaaacc ataaaagcgt gtcgtgagtt gactgaactc
    46621 agcattgccg gggaaatatc cctggaacaa ctgcattcgc tgctggcagg ggctgtcact
    46681 gatgagttga gtggcgtgga cgtgatgact gaagagcttg agttccgaat caaaagcatt
    46741 ctgacaggta aagcctgatg aacaaagcca aagaaaagtc tccttcactg atacaaatga
    46801 cacctccccg caaaattgat ctgtccggtg aaaaagccct gctgggcgtg gatgtgccgg
    46861 acagccttga tttgcccggt gatatgccgg tgtttctgga ttatcaggcc cgctggtttg
    46921 cagatgaaag tcaggtctgc atcgcggaga aatcccgacg caccgggctt acctgggcgg
    46981 aagccgggcg taacgtcatt accgccgcaa agcctaagcg tcgtggtggc cgtaatgtgt
    47041 tttatgtggg gtcaaaacag gagatggcgc tggaatatat ttctgcctgc gcgctgttct
    47101 cccgtgcctt taaccagctg gcagatgctg atgtgtatga gcagacattc tgggatcggg
    47161 ataaaaaaga agaaattctg acctacatga tccgctttcc gaacagtgga ttcaaaattc
    47221 aggcattgtc ttcccgtccg tcaaacctgc gcggtcttca gggggatgtg gtgattgatg
    47281 aagccgcgtt ccacgagtca ctggatgagc ttctcaaggc ggcagtggcg cttaccatgt
    47341 ggggcgcacg cgtgcgcatt atttccacgc ataacggcgt cgataatctg tttaatcagt
    47401 acattcagga agcccgcgag gggcgcaagg attacagcgt ccaccgcata accctggatg
    47461 atgccattgc ggacgggctg taccgtcgta tctgttacgt caccgatcag gaatggtcac
    47521 cggaaagcga gcagaaatgg cgtgatgatc tctacaaaaa cgccccgacc cgtgaggatg
    47581 ccgacgagga atacggctgt atcccgaaaa aatccggcgg tgcctatatt cctcacgcgc
    47641 ttattgaaat ggcgatgatt cgtgacatcc cgattctgac gtttgaagcc ccggacgact
    47701 tcatcagccg tgccgcatgg ctgcgtgaat cggaagtttt aacctggtgt gaagaacatt
    47761 taaaaccgct tacagagaaa ttaaatcccc gttcccgctt cagcttcggg gaagactttg
    47821 cccgcaccgg tgacctgtcc tgctttgtgc tgctggaaat caccgaactc ctggcaaagc
    47881 gcgaggtgtt tcgcgtggag ctgcgcaacc tgccgtatgc ccagcaggaa caggtgatga
    47941 tgtacatcct gacccgcgtt ccggcgctgg tcggtgcggc gttcgacgcc accggtaacg
    48001 gcggctatct ggcagaggcc gcactgctgg cctttggccc ggacatcatc gactgcgtga
    48061 tgttgtcgcc gaagtggtac ggcgagtgga tgccaaaact gaaagccgag tttgaagatc
    48121 agaatatcct cgtcgcccgc catcagacca cgcttgatga tttgcgccat gtgaaggtgg
    48181 tgaatggtat cccgcaaatc gacaaggggc gcacgaagga ccagaacgca acggctgcga
    48241 acgcccgccg ccatggtgat tttgccgtgg cactctgtat ggcaaaccgg gcgtcataca
    48301 tggagggttt catcctggat gaatcagcct gtcaggcgct gccggagcgg tcgcgggcaa
    48361 tggagggcgg ttatcgtgat gacgatgagg catatcatga atttgatcgg gggtgctggt
    48421 agtgggacgc ataattgatc ttgacggaaa acctttctcc tttgacccgg agatgcaaag
    48481 taccgcgctg gatattccgc agattgccag ccgttatgtt gagcatcccg cctcgggtat
    48541 caccccgaac cgggcggcgc agtgccttcg cggggctgaa cgtggcgatc tgattgccca
    48601 gtccgatctg gcggctgaca ttgaagaaaa ggacacccac ctttttgcgg agctgggcaa
    48661 gcgacgtctt gccattcagg gggtgccatg gagcattgag ccgccactga acgccagcgc
    48721 gaatgagaaa aaggacgcgg aaatgctcga cgaatatctg cattccgccg actggtttga
    48781 tgccatgtta tttgatgcca ccgatgccat cctgaagggc tattcctgca tggagattga
    48841 gcacgggatg ctcggtaaga tgcacatcat ccgcgccatc cgatggcgtg acagcgggca
    48901 tttctgcctt aacccggatg atttgagcga actgcggctg cgtgacggca gccatgccgg
    48961 ggtggcgttt cagccctttg gctggatagt gcatcagtcg cgttcacgca ccggctacgg
    49021 tggtgcgacg gggcttgtca gaacgcttat ctggccgttc attttcaaaa actattccgt
    49081 gcgcgatctg gcggaatttc tggaggtgta cggcctgcca atgaaggtcg ggaaataccc
    49141 gtccggggca acaccggagc agaaaagtgc cctgatgcgg gcggtgatgg atatcgggcg
    49201 acgtacaggc gggatcatcc cggccgggat gtcgctggag tttcaggcgg cagcgaacgg
    49261 tcaggccgat ccgtttgaaa ccatgatttc gtggggtgag cgttccatct ccaaagcaat
    49321 actcggcggc acgctgacca cagaagccgg agacaaaggt gcgcgctcgc tgggtgaggt
    49381 gcataacgag gtgcgccggg aaatcaggga ttctgattta cgccagctgg ctgcaaccct
    49441 gaaccgcgat ctggtgtatc cgctgtatgc cctgaacacc gcccacgcca ttgatatccg
    49501 tcgcctgccg cgtatctgtt tccagaccaa agaaccgggg gatatcacca aaatagctag
    49561 tgcggtgatg caactcagtg cggggatgga tattcctgat ccctgggtac gggatcagac
    49621 cggtattcca cagcccacgc ccggtgaagc catattccgt gtccgtcaga gcagtaatga
    49681 accggccatg cctgacaggg aaacgctacc ggaaaaacag gaaaagacag aacagacggc
    49741 gctttcagcc cgactgccgg aagcgaaaaa cacgccgcgt gatgaactgg acgacatggg
    49801 ggacgcggtg cctgcccgcc gcttacagga ggccatcgat ccggtgctgg agccggtcat
    49861 tgatgtcatc agaacgcggg ggctggcgga ggcactggca gacctgcctg ccctttaccg
    49921 tgaaatggat gattcccgcc tgatgacgct gctcagtgat gccatgtttg ctgcggaaat
    49981 gaaggggatg ctggatggct caggggattg atttaggtta tgccgccacg cttccctcaa
    50041 aagagacggt ggcatacttc cgcgccaaag gggcgcatat cagctggaac tggttcgaaa
    50101 cagacgcgga tgttcatgcc cgctcattca cggcagcaaa aacggcacgg ctggatgtgg
    50161 taaccacatt acaggccgaa gtgcagcggg ccattgatga ggggatttca cagaaagaat
    50221 ttatccgcac actgacgcca cgcctgcaaa agctgggatg gtggggaaag cagattgtgg
    50281 tggacagcga cggtaacgcg gaagaagtgc agcagggcag tccccgtcgt ctggcgctga
    50341 tttacaacgt gaacacccgt gtggcttaca atgccgggcg ttacacgcag atgatgaaca
    50401 acacggacac gcatccgttc tggcagtatg tggcggtcat ggacagccgt acccgcccgt
    50461 cgcattccgc ccttaacggt ctggtattcc gctatgacga tccgttctgg aaaacacact
    50521 acccgcccaa tggctggaac tgccgctgcc gtgtgcggcc attgtctcag gcccgtctgg
    50581 atgcaatggg gttatccgtt tcatccggtg aggatcatct ctccacccgc aatgttgagg
    50641 ctggcgtgga taaacagacc ggagaagtca gagaaatgcc ggtgaccaca tattcagatg
    50701 gcgccagaac catgacaccg gatgtgggct ggtcatataa ccccggctcg gcagcgttcg
    50761 gcacagacca ggcgctgatc cgcaaactga tcgaggtgaa aagcccggcg ttacgggaaa
    50821 tggtggttca ggagatgaac aacagcccgg agcggcagct ggcgttccgc atctgggcaa
    50881 aaaacatcat gcagacccgg cgaggtggta acgatatccg cacgctgggc tttatgactg
    50941 aaagcattgc gcaggcagtg gaaagccgga cgggaacgcc accggcccgc ctgctggcga
    51001 tgagcggtaa aaatgtgctc catgcggaca gtgtgaaaca tcagaatgac ggcatcgctc
    51061 tgacgccgga ggacttcggg cgcttaccgg caatgctggc aaggccaaaa gcggtactgt
    51121 gggacaaacg gcacaacaat ctgatgtata tcgtggaaaa tcaggatggc agtgtgcaga
    51181 ttgccgttaa tgcaccgtac agtctgaaac gtcagcccga caaactggat gtgattgtga
    51241 atgcttaccg ggttatcaat atggataagc tgaaatcgga tatccggggc ggaatgcttg
    51301 aggtgctgga aggacaaatt gattaacggt cagtggcggg ggtcgaaccc gcatacatga
    51361 tccacccgga ggcaggcacc gactttacca gttaagcgta cactgaccgc ctgtttattt
    51421 tatattcatc ctttatgcgg aggcaatatg tcatccattg atgcagctgt cgttgttgac
    51481 gttacgcgtc tccagcgagt gtttgcccgg cttcagtttg tgggcggcgg aaaagacctg
    51541 gcccgcagtg tggcggcgag tcttctgtca tcgtcagaaa tggcgtttga gcaggaaaaa
    51601 gagccggatg gtgaacgctg gcatgactgg tcagatcctt accgtgagtg gcgtacccgt
    51661 aagggataca cgcccggcaa aatcctgacg ctgaacggcg atctggcccg ccgcctgacc
    51721 acggattatg gcgatacctg ggcgctgatt ggatcaaatg aaccttatgc agccattcat
    51781 cagtggggtg gcctgcctgg tatgccaccg ggaccggcgg ccattggcgc acgtccgtat
    51841 atgggctttg atcaggtggc agaacaggag atcatggacg aaatcagaaa acgctttaaa
    51901 aaggccacag aaacgccgta atggtttaag gtatgcaaac gtattacctt acccccttca
    51961 ggcgcgtgtc gtgattttct aacctgtatt taacgggctt taaaatctgc gtgaagcgcc
    52021 tttctcattc cccccctgtc agcactttct gaagtcccgc aaaaccagcc ccaaaaatac
    52081 ccggcttatg ctgccggtat gaagacagaa aatcacccaa aagacgcaaa tcgcccctcc
    52141 ggtttagccg tgctttcagc ttcgctgact gcatccggcg acggatggtg tcagttactg
    52201 ccagccggtc gggtaaaggc ccgtgacggt cgcccggaaa aaccgactga aggctggctg
    52261 attaacgaat ccgcctgtaa ccggatgaaa gccgggcttt ccgccctgca tcagccgtta
    52321 ctgattgatt atgaccatca ctccgtgaat gcgcagaaaa acggattcaa ggctattgct
    52381 gcaggatggg ttaagccgga aaacatcgaa tggcgtgagg gtcaggggat tttcattaaa
    52441 cctgaatgga caccacaggc acaaaaacat attgatgacc gtgaatatgc ctatctgtct
    52501 gcatatatgc attatttcgc tgacaccggg gaaccctacc tgttgcgcat ggcttccctg
    52561 acgaatgatc cgggtattac gggtatgaac cctgtcgcgg cactcagtgc tgatgatctc
    52621 tatgtcgttt ccccttctca ggagcaatcc cccatgaatg agcaattacg ccagttgctg
    52681 acggcgctcg gtctgaccgt ggctgacggt gacgaattta caccggaact gggcacggcg
    52741 gcgctgtctg cccttaccgg gatcaaaacc cgtgcggatg cgcacgacac cctgaaaacg
    52801 caggttgcca gcctgtcggc agaactggaa accgcaaaag gcacaccaac tggcggcact
    52861 attgatctga cgaaatatgt gcccgttgag acgtataacg ccctgcgtac cgaatatgcc
    52921 gcgctgtcgg cacaacacgg cagcaccacg ctggagcagt tgctggataa ggccgagtcc
    52981 gaaggacgca ttttcaaaag cgaacgcgga tacatggaag gactcggaca acagattggc
    53041 gtcgcggcac tttctgcaca gcttgacgcc cgccagccgg ttgcggctct gaccactctt
    53101 cagaccgaca ccgtaaccgt gccggataaa aagaccgcca ccgctgtgct gtcggctgaa
    53161 gatatcgccg ccgcacgtct gttgggtaaa accgaagccg agttcctgaa aatgaaagag
    53221 gaaatgcaat aatgcctacc ccgattacac cggcgatgat caccgccctg atgacgggct
    53281 accgttctga ttttcaggcc gggatgtcca tggctccgtc gcagtaccag aaaattgcga
    53341 tgaccgtgcc gtccacctcg aaatccaaca cctacggctg gctggggcag ttcccgcagt
    53401 tccgtgagtg gatcggctcc cgcgtcatcg agaagatgaa agcctatggc tacgccatcg
    53461 tgaacaaaac ctttgaaggc accgttgcca ttagccgcga tgactttgaa gacgacaatc
    53521 tcggcatcta ctccccgtta ttccaggaaa tgggtcgtgc cgctgcggca caaccggatg
    53581 aactggtctt tgctgcactg cgtgatggtc tgaatgcggc ctgctatgac ggccagaact
    53641 tctttgatac cgaacaccct gtttacccca aagtggatgg ctccggtgat gcacagatgg
    53701 tcagcaatat gtttgtggca aaaaccggct ctgtcggtgc acaggcggat tacagcggcc
    53761 cggcctggta tctgcttgac tgctcccgcg cggtaaaacc gctgatttat caggatcgcc
    53821 gcaaagccga actggttgcc cagacaaaag tcgatgaagg ccgtgcgttt actgataacg
    53881 agtttgtgtt cggtgcttcc gcccgtcgca atgtgggcta cggcttctgg cagatggcct
    53941 acatgatgca gtcaccgctg acgctggatg cgctgtggca cggctggtca gccatgcgtg
    54001 actttaaggc tgacggtggc cgcaaactcg gcatcaaacc cacccatatt gttgtcccca
    54061 cctcgctgga aaaacaggcg gtgcagctgc tggagcgcga gctgttcgca gacggtaacg
    54121 ccacagtctc taacgagatg aagggcaagc tggagctggt tgtcgcggat tatctgtaaa
    54181 cggtgagccg ggcttacggc ccggcccctg cggagggcaa atatgaatga acatcacact
    54241 gtggcagcaa cagatgattc aggccttcag gtcagtggtg acagtcctgt cacggtactg
    54301 gcagaagtgc gttgcagtcg tacgcatttt cgtcgtgcgg ggttcctgtt catgcgggga
    54361 cgccagcagg ttgacgtcac cccggagcag cttgcccgac tggaagcgga gccatgcctc
    54421 actgtacgaa tactacagac gcctggtgat gatgcgggag ccgtggcggg tgtggttcat
    54481 gcagagaccg gtgcagattt agccgaagcc gaaaccgtac aggatgctcc ccggaaaaag
    54541 accggcaata acgccagaca ggcgaaaaag gcgcgcgcat gaattacgcc accgaaaccg
    54601 atatgcgggc acgttaccgc gaggatttgc tcaggccatt actggccgtg ccccgttcgg
    54661 atgaaccgga cacgcgcaag ctgaaccggg cactgacgga cgcgtctgcc cttatcgaca
    54721 gctatctgtc ttcccgttac acgctgccgc tggcggtggt tccggcggtc cttgttcagc
    54781 attgctgcac gattgccttt tattacctgt gcgatcagcg ggcatccgat caggcgcgtg
    54841 accgttaccg tgaggcgctg gcctggctga aagatgtcat gaacggtaac gtgccggtcg
    54901 gcgtggatac agacggtgcg gcccctgaat ccggggattt accgcaggtg cagtctgatg
    54961 cggcggtgtt cgggcgcaac cagaagggct ttatatgatt acggaaaccg agcaggcgta
    55021 catcgcccgt atccgtgagt atttcgggaa tgaactggtg tctgttgaca cacatcccgg
    55081 cgactggagc gacagcgtac tgcgcaccat gctgattaac gccccggcta tctatgttgc
    55141 ctggctgggt gccggtgaag ggcgtacccg tgggcgtctg gtcagtcact gggtgttcta
    55201 cgtcatcggc gacatgctca atggccgtga ggtcagccgt cccgggcttt atcagattgt
    55261 ggcccgcctg attaccgtgc tggacggctt cagagcagaa aaaacctcac cgctttactt
    55321 tgaaaaggcg gtcaacggtt acaccgaaac gcaggcaggc agcggtgccg tgatgtacgc
    55381 cctgtatttc tcatgcgagg aaatgatcga cccgctgacg gatatcagct cgctggatga
    55441 tttcctgcgc cattacgaaa cctttaccga accggaaggc acgccggaat ttaaggcaca
    55501 catccgtctg cctggtgcaa ctgccgccgg tgaaaacgcc ggactgtcag aggaataaca
    55561 gatgacaacc atttttatta aacctgcgcc gggatgcctg atccgcgatc ccgacacgat
    55621 gactccgctc tctcaggacg gggaagagaa acccttcact ccgttctggt gccgccgtct
    55681 taatgatggc gacgtcattg tggtggataa aacggcggag cctgccccgg ctgccacaac
    55741 tgctaatacc ggcgcggcgg caaaaccagc gggagcggta gcagaaaaaa ctgcggcggt
    55801 ggctgaaaaa accgcagcat cagataaggg gacgtcctga tgattaactt tgattatatc
    55861 ggcgataaca atcgcattcc gctggtacag attgaaatca ataattcaat ggccgtgacc
    55921 ggcactccac cacagcgtca ggcggtactg ttgtttggtc aggctgcaat gaaagacact
    55981 accattcagg ggcgcggtca gcttgatgta ccagtacgta ttacccgagc ttcacaagca
    56041 agagaactct gggggcgagg ttccatgatt gcactgatgg tagaggaatt tatcgccatt
    56101 aaccctgaca cagagttata tgccattgca cagggggctg gaacaggtca gtccagggca
    56161 tgcgtgatga atatcattgg aacagcattg gcagacgggg tgttaagtgt ttacatcggc
    56221 ggtcgccgtt acctcctgcc cgtcagtaag gggaaaaaag gtaaagagct tgtggaagag
    56281 ctggctaagg tcattaacgc tgacactgat gcgccattca ctgctatcgc agctgaagtc
    56341 agtggtgata atgctgaagg acttaaaggc agcatggggg ttaacgcccg ctttatcggt
    56401 gaatgctcag ctcatgattt acgggtgaac tattatgacg gtgaaactac accggccggt
    56461 cttacgctgg agctcgtcgc gccaacacag aaggcaacaa accccgatat cacccgtagc
    56521 gtctcaggga tggggaatcg tcagtacaac tacatcgtca tgccctataa ggatgcggca
    56581 aatctcaaaa ttctggcgga tgaactgctg aaacgctggg gaccggtaaa aatgtcggac
    56641 ggtatggtct ggatggcgca taccggcacc ttcggtacgg caaccagttt tggtgaatcc
    56701 cgtaatgact tcctgtttac ctgcacttct attcctaaag caccagagcc ggattatgtc
    56761 tgggctgcct ccatttgtgc ggtctgtgcg ccctccctgt caacggatcc ggcccgacca
    56821 ttacagacac tggcgctgcc tgcccgtatg gcacctgaag caccggatcg tctgacgcgg
    56881 gaagagcgaa acagcctgct ttataccggc atgtccacgg tgaccgtcgc ggccgcggat
    56941 gtggtgcaga ttgaacgtca ggtgacgatg taccgtcaga atgcatacgg cgaaatcgat
    57001 cccagctatc tggatgtgga aaccatctac accctgtcat atctgcgtta ttccctgcgc
    57061 acctttatca cgcagcgttt tccgagccat aaactggctg atgacgggac gcccgttcgt
    57121 gccggtcaga atatcgtgac accggagatc atgaaacttc agttaatttc gctgggggaa
    57181 gaatgggtgg atctgggact ggtcgaaaat ctggacacct ttaaaaagaa tctgctcgtg
    57241 gagcgcaaca caaaagaccg taaccgtctg gatgtgatgt gtacaccgga tctggttaac
    57301 cagttccgct tcatggccgc gcagatccgt ttcattttgt gaggtaaggc atgagcggaa
    57361 aacagtatca gggcacggcc accatccgtg tgaacgggca ggagtacgac acccttgagg
    57421 gggccacgtt ctccccgtcc ggctttgagc gtgaagtggt gaaaggcgcg aaagtttacg
    57481 gctatcgcga gaaaccccgt gaggcgacgc tggactgtaa attcccggcg ggcggagaag
    57541 gttcaccggc tgccgatgaa atcaacacct ggactgcggt cacgattgag ttcgtggccg
    57601 ataccggtga agtccacatg atgacgaagg cctggagcag tgaaccggcc tcgcttgacg
    57661 gtggtggtga tatctccgcg aagttcgcca gcgccaccag tacccgtgtc cagtaatcag
    57721 gaaaaatgta atgagcacac gtaagaaaaa aacggcggtt tctgacgagg ccgtgatgga
    57781 ggctatccgg gatgcacttg aagacggcga tccccgcacc gccgggctgg ctgaacaact
    57841 ggcgaacggc tatgtggatc tgctcgacgg tctgccgttc ggggaatccc gtgaataccg
    57901 cgtcaccttc cgcgaactga cggcaaaaga cagcattgat gcggagagcg aggccgagcg
    57961 tcttatggaa acccgaaacg gtccggttct tgtggcctca ccggcactgc gcggcattgc
    58021 gctgctgcgc cgtcagattg ccgctgtcgg ccagattgag gggccattgt ctccgcgtca
    58081 gataggacag ctcagcgagc gcgatctctc ccgactgatg gcggcagtaa gcctgcttga
    58141 ttccgcgatg gccggaaagc tggtcgctga acgggggcga ccagatgcag tgccgggatc
    58201 agattgaaga agccgcaatt gtgctgggga tggtgacaaa aagcggcccg gagtgggcgc
    58261 ttaacctccc cttatcgcag ctttaccggc actgccgaca gaccgaaaaa attctcagaa
    58321 cgaagcagta acccatggca aaaaacctga aagcctccct gattgtcgat ttactcggca
    58381 atatttctgc caaatcccgc cagtggtcac aggagctggg ggcattctcc cgttccggca
    58441 ggacggggct gggtggtctt ggaaatgcag cacgtcgtgc cggtcaggag acagaacttg
    58501 tcggctcccg tatgcagcga acgcttgccg gtgttcgtgg cagtatccgc acggtgacgt
    58561 cagattttga ccgccttcag ggcagcatta ccggcaccat cgggcggata agcaaccttt
    58621 acgggatgct ggcgggaggg gctgcggtct acgggtttaa taaagccttt atccgcccgg
    58681 ccgcagagat ggagaactat attctgcgtc tcaacgccat taatcatggc gatacggcaa
    58741 aaacggaagc agtgaaagcc tgggcggtac agaacgccaa agacaccacc tgggggctgg
    58801 cgggggttat gcaggaatac gcctccagtc gcgggttcgg tatgagtgac agggaagccc
    58861 gtcgctttat taccatgctt caggatcagg gcggctatca cggctggtca ctgtcagatg
    58921 cacagggtgc gtccctgcaa ctcaaacaga tgtttgcccg ccagagtatc caggcagcgg
    58981 acgccaatat tctgaccgga tacgggatta acgtttacca gttgctggcc gataagctgg
    59041 gtgtaaacca gaagatcatc cgtgagaaag gcgagaaagg aaaactggga ccggacagca
    59101 ttcgcctgtt atttcaggtg atggccgaac aggcaaaagg tgcccagaaa aacgccatga
    59161 attcctggac gggaatgacg tccatgatgg gcgatgtctg ggacggattt gcccgcgagg
    59221 tgatggcaaa gggaccgttt gacagcctga aaaagagcct gaaaggcttt ctggattatg
    59281 ctgatgcggc acagaaaagc ggcttacagg ataagctggc cacacagacg gcctcagccc
    59341 tgaatcaggg atttgagtat gccagagacg ctgcgaccgg cttttaccgg gtcattcaga
    59401 aagtcaggga aacgcttcag gcactgcgcg acgccggtta tggcgatgcg ctggatcgca
    59461 ttggtcaggg tgcgcagacc gccgcaaagt acctgatgta tatgtatctc gcctcccgtg
    59521 cgctgaaggt gctcaggttt gcggggacgg gggcattacg tctcggcgca acccccttac
    59581 gctatggcat ggcgatgacc tccgtgctga cgtcgccctt ccgcaaaccg cagaccactg
    59641 taccgggaac gcagcccgga cgcgctggcc ggttcctgaa tttccttacc ggggtaaatc
    59701 cggctgccgt tcagcccgtg ctggtcacca actggcctgc gggtggcctg gcctcaggtg
    59761 gcggggatgt ggtggtcagc ggtgacggca aaaccgtgcg cggccgtaaa aaacgcggcc
    59821 ccggacgtgg acggggtgtc acgaccgtcg tgactgccgg tgagcaactg gcggagagtg
    59881 ccggtaaaca gggcttcttc ggtcgtatga tgagccgtgc cggtggtctg ctaacggcgg
    59941 ccggaaaccg catggggctg gggcgttttg ccgggctgtt ccgtggtgcc ggtcggctgg
    60001 gtggtggtgc cctgtgggcc ggtgccatgg cggctcctgt actgctggac agcagcgcca
    60061 gtgcagccga taaagccggt gccgtgggtt cccttgccgg aagtatcgcc ggtggcgcgc
    60121 tgggggcggc tgccggtccg gtcggggttg ccattggttc cacggtgggc agctatctcg
    60181 gtgattatct ggggggctgg ctgacgcagg cctggcagaa actgcgtggc ggcagtgacg
    60241 aaaacggcgg acaggccacc gcgaaaaccg ccgcacgggt ggagcttgtt gcccctgaag
    60301 gctggcgggc gcgcagtatt gatgtggatg ataccgcaca gcatggcctg gatgtgaacg
    60361 tctggaacgg agggaactat ggcctctact gacacgccgg ggcgtggctc ctttcgtggc
    60421 gtaccgttcc tggtttatca ggagcagcgg gaacggggcg gacgtaacat tgtgcgccgt
    60481 gaatacccac tgcgtgaaag cggcggtgcg gatgatctgg ggccaaaact cccggagttc
    60541 acattcacgg ttctggtgac cggagacgat cttcagacac agcgaagccg cctgcgtgat
    60601 gcgctgcgtg cacccggtgc cggtgaactg atgcacccgg attacggcac gttaaacgtg
    60661 ctgataaaca gctttgaaag ccgttataac gccagcgagc agggaacggt tgagttcacg
    60721 atcaacgtga tccccgccag tgacgatacc gcccctacgg tggcagaaga tacggcagct
    60781 gcgctggagc agaaaagcgg ctcagcaatg aatcagctgt ttaacacgct ttcagacggc
    60841 tggacggtga tttcagacgg tctgcatgat gtacaggcca tgaccgacac catcagcgat
    60901 aagattgatg cactggagaa tgccgtttcg gggatgggga ttgtgcagga tatcagtgcc
    60961 tttacggcca gttttactgc cctgaaaggc aatgccgccg ccctgattaa tgcgccccgt
    61021 cgcatggctg aatcgctggc cggaatgttt gccgtgctga ccgggctgcc cggcgatcct
    61081 tccctgtctc tgaccggaaa agccggaacg ccgtccggca gcctgaccac aaaccgtgac
    61141 agcatggcag agcaggccat gccgcagctg taccgcacac tttcctccct gcgttacacc
    61201 ctcagcgaac aggatgatcc acagcgcctg attggcctga cgcctgcggc acagaaaaat
    61261 atccgtctgc tgcggagcgt gatgcagagt gcaaccctgg tgtcgcaggc acagacgctc
    61321 ggaaaactgc tggatcaggt catccggcag aatacgacgc acgttcagga tactgaccgt
    61381 tcgggcggac tggcctggct ggaaagttct gctgatgtgc agcgtatcaa ccgggatttg
    61441 agcgatgcca tggaacaaca ggtgctggat ctgtccgcac agggctatac cgggactgcc
    61501 cttgcattac gtgatgcagg gcttgcgctg acagaagatt taaccacacg gagcgtgcat
    61561 cttcccggtg cttttcaggt catggttcgc accaccgaac cggcactggt cacgctgtac
    61621 cgtgccaccg gcaacagcag tcgctggcag tattttgtcc gcagaaacaa tatccccgat
    61681 ccggtttttg ttccgggtgg ccgcagcgtg gaggtgatca gtgagcagca ggattgaact
    61741 gtatatcggt ggcagtattt tttccggctg gctgacggtc agcgtccgtc gctcgctgga
    61801 gcatctggcc gggtcgtttg aactggggct gatgctgccc ggtgagcgca tcccctcagc
    61861 cctgcgaacc ggtcagtccc tgacgctcag aattaacggg cagacggtca tcagtggctg
    61921 gctggatcag gtcagtcagc ggatcagtgc aacccgtcat cagatcagca tcagcggtcg
    61981 ggataaaacc ggcgacctgg tggactgtgc cgccatccat cccggcagcc agtggcgcaa
    62041 ccggacgctg gcacagattg ccgcagattt atgtgcgccc ttcgggatag ccgtgcgctg
    62101 gcaggttaac gacgacacgg ctgcacgccc cttcagctct tttacgctgg aaaactcaga
    62161 aaccgtggcg gatgcgctga cccgtgctgc ccggcatcgc ggggtgctgg tgaccagtaa
    62221 tgctgacggc gatctggtgt tcacccaggc aggaagccag cagacggaca gactggtgct
    62281 gggagataac ctgcttgatg ccgattacaa cacggactgg cgaggacgat acagcgaata
    62341 ccgtgtccgg ggacacgggc gcggtggtgg caaacgggga gacagcgaat ccgccgcccg
    62401 tctggcagca cctgtgggcg tcatcagtga tgagcagatc ggccgctacc ggtcgaaaat
    62461 catcctcgcc gatcaacaga cagacaccac cggtgcacgg cagcgtgccc tgcgtgaaat
    62521 gcgccgtgcg attgcccgtt cagaacggtt ttctgccacc gtgcgtggct ggttccggga
    62581 tgatggccgg ttatgggatg tcaatctgct gaccggtgtt tcagccctgc gtttcggtat
    62641 agaacagact gaactgctgg tctgtcaggt ggagttttta cttgatgaac ataacgggga
    62701 agtcacccgg ctggtactgg caccgcgtga cggctttatc gttccggcag agccggacag
    62761 taagggccgg ggtggttccg gtgacgatgt tgatgccttt attcgccagc agatgaaaaa
    62821 acaggggatc agctttgatg aatgatgaag tgttcagccg cctgattgcc ccggtaacgc
    62881 gcggtattcg cctgttgttt ggccggggcg ttctgaccgg cacacatgac gaactgaaaa
    62941 tgcagaatgt gcagctcacc ggcatggacg gcgaaacctt tgatgatgtg gagcgtcccc
    63001 agcagtacgg gcagatcagc gttcccctgc cgggtgcgga aacctttttt gcctgtctgg
    63061 gcggacagcg ggatcagact gtggtgcttg tggtggaaga ccggcgcagc cgcccgaccg
    63121 gactcacagc cggagatacg ggggtgtatc accatgaggg gcaccggata cggttaacaa
    63181 aggatggtcg cattattgtg acatgcaaaa tgctggagat ttacgccgat gaggggatgc
    63241 gggtggatac gccggaggcc acctttacgg gcaatgtgac ggtggataaa aacctgcatg
    63301 ttaagggtaa tttcgtgctt gatggtacag gtaaatcaga cgggctgttc acgatgtctg
    63361 atgccgttat tgccgggatc acctattccg gccatgtgca tcaggataac ggcaaaggca
    63421 gtaagacagg agcgccggag aatggctgat attgcaattg tatgggatca ggggtgcggt
    63481 tcgctgcaac tgaacggtgc cgacttgctg acggatgaca gtctgctgac ggccgttctg
    63541 atttcgctgt ttactgaccg ccgggcgctg gcatcggatg aaatccctga tggtacgcgt
    63601 gaccgccggg gatggtgggg agacagtttt cgcccgcgtc ccattggctc ccgtctgtgg
    63661 cttctccgcc gggaaaaaac actggcctcc gtgataagcc gtgcccgtgc ctatgcggat
    63721 gaggcgctgg gctggctcag tcaggatggc gtggcgtcat ccgtggtctg tcatgcagaa
    63781 cgtgtggggc acgcgcggct ggcgctttcg gtgcgcataa ccctgcctga tggttcagtg
    63841 aggcccatga ttttttatgc tgatcttaag ggggagtaat gccttatcag cctttaccgc
    63901 tggcacagtt aatcacgcag acacagcagg atatcagcca gcgcctgccc ggctcgcagc
    63961 cgggtgtgaa tgaaaccacc ctgaatgcca ttgcttacgc tcaggcaggg ttatctgctc
    64021 aggagcatga gcatctggcc tggattgcgc gtcagatcat cccgaccgaa gccgatgaag
    64081 ccgaactgct gaaacactgt gcattctggg gcgtcatgcg taagcctgcc tctcgggggg
    64141 acgggccggt tcaactgatg ctgaccacgg atgcggggat caaggaaggt gtgcttctcc
    64201 agcgaagtga tggcgtggtt taccgtatca ccacctcact gaccggtaag gccggtacga
    64261 tgaatgtcag tgtggaagcc gaaagtgccg gtcgtgcggg gaatgccccg gcagggacaa
    64321 aactgacatt tatcacaccg caggcgggga tcaaccagac ggccacggtg accggaacgg
    64381 ggatcaccgg tggtgcggat gtggaaaccg tgccggagct gctttcccgt ctggttttcc
    64441 gggtgcagaa tccgccgtcc ggcggcacgc agtatgattt tgaacgctgg gcgcgtgaag
    64501 tgccgggcgt gacgcgggca tggtgccgcc cggaatggcc gcaggcgggc agcgtggggg
    64561 taacgtttgt tcaggataat aacccggata ttttccccgg tgacggtgat gttcagcggg
    64621 tggcggatta tatccgcagt catgatgatc cggcgaccgg ccagcctgtc ggacagcctc
    64681 tggggccgac ggtgaccgtg tttaaactga ccaataagcc ggtgcccttc agcatcagga
    64741 tcatcccgaa aacaccggag aatcaggcgg ccgtaaaaca ggcgctgacc gacctgttgt
    64801 ataacgaatc ccggcccggc ggtctggttc tgccgtcgtc tttctggcgg gcggtggcag
    64861 gggtgaaaaa tctggaggat tttgaagtgc gcagtccgct gacgtcagtt caggcgggag
    64921 acagtgagct gctgacggta ggagaaatca catggctgta accctgaccc cgcatcagcg
    64981 cgccctgttg cagttactgc ctgacgggct ggcatgggac aagcgtccgt catccgtact
    65041 ggcatcatta tgtctgggcc tcagtcattc gacagcgcgt gtatcctgga cgggtaaaca
    65101 gctgcttgcc gagcgtttcc ctgacacgtc gcgcctgctg ctggaagact gggagcgtta
    65161 tctcgggctt cccgagtgtg atatggccgg agccaccctc acggagcgac agcgttatgc
    65221 cggaaataaa taccggatga agccctcgct gaaccgtgaa ttttatatcc ggtttgcggc
    65281 ggagtttggt tatcagattg acattcagcc ctcaccggat tcgcagtggg tcagtattgt
    65341 cacgattaac agtgaaaccg gctaccggaa tatgaatgtg ctggatgata ttctcacgcc
    65401 gctgcgtatt tatgaaggcg gtgcgctgga atgtattctg aaccgttata agcctgcatg
    65461 gcagacgttt atttacgttt atgcaaacag ccatgaagag gagactattt aatgtttcat
    65521 gttgataata attccggcgt ggcgaatatg cctgcgctgg caccggcgca gagtaatacc
    65581 accacctggt ttaccgaagg tgacggacaa aaaggtatca gctggattgg tcaggactgg
    65641 ctgaatattc tccaggccga actgctgaat attctggctg aagccagtat tcagccggat
    65701 aaggcgcagt taaaccagct tacgctgtcc attaaagcca ttatcgctgc gaatgccttt
    65761 tcccggaaaa ataacctgaa agaaattgct gatgccggtg cggaggccca gcgtctggcc
    65821 cgtggttatc ttggtctggg gacgcttgcc acaaaaaaca gtcttggtcc cgctgacgtt
    65881 aatgccctgg cgaaggatca gaatctcgcc gacctggaga ataagggaac cgcccgtaat
    65941 aatctggatg tttacagcaa aagcgaaggt gataaccgtt acctgcgcag ggagcagaac
    66001 ggcgcagaca ttccggataa aggggctttt atcgataacg tcggtttacg ggaaacggta
    66061 aacagggcgg cgaatgccct gccatcggac ggcaccgccg ttgccgcgaa caggctggca
    66121 aatgcccaca caattaatgg tgttcccttt gacgggacgc aggatatcac tatctcatca
    66181 ggaacagtaa ccgccataag actggggtcg gtgaccgcac atatgcccgg aacatgggaa
    66241 agctgggatc tgaatctggg gggcggcaat gttcttaccg gaattaaagt acaggatgtc
    66301 ggtaagaata cggcagataa cgtcggcggc gtgtattacc ggccgttgca gtatctgtta
    66361 aatggagcat gggtgacggc tgcaagtata taatgatatt cagaaggcct tccggccttc
    66421 tttattactg tggctgctca ggccatttaa tggcgttaaa ttcagcttct gttttaatgg
    66481 cgggtagttc cattttcttc acctgactga tgtatgccat ccattgggta agtgctgctc
    66541 tgtcttcatc gctgatggtg cccagttgta gctccgttct ccagtcatta attttttcat
    66601 atgcctgatt aagatatgac tggcgcattg cttccagttt tcttgcgtag ttaaccggaa
    66661 tacgggagat tatgccatta tcaaacttcc agtttcctga aatatccgtg ccttcaggga
    66721 gttcatcgac ctcaaccaca gaaaaccccg caggatacag cgccgaggcg tcctgtgata
    66781 ttgagcagat aacgccgctg tcaggtgtta tgcaaagttt atattttttg gtgaataatg
    66841 gcagggattc gtagaaatcc ttaccatctt cactctggaa atactgaaca ttattaccat
    66901 atggcat
//
LOCUS       DAZGJT010000012        65731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZGJT010000012 DAZGJT010000000
VERSION     DAZGJT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.012"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(219..1079)
                     /locus_tag="ACHKFK_000859"
     CDS             complement(219..1079)
                     /locus_tag="ACHKFK_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO9051267.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     gene            complement(1262..1819)
                     /locus_tag="ACHKFK_000860"
     CDS             complement(1262..1819)
                     /locus_tag="ACHKFK_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9051268.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            1983..4376
                     /locus_tag="ACHKFK_000861"
                     /pseudo
     CDS             1983..4376
                     /locus_tag="ACHKFK_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            4599..5216
                     /locus_tag="ACHKFK_000862"
                     /pseudo
     CDS             4599..5216
                     /locus_tag="ACHKFK_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            5814..6056
                     /locus_tag="ACHKFK_000863"
     CDS             5814..6056
                     /locus_tag="ACHKFK_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO9051269.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(6088..6738)
                     /gene="tetR(A)"
                     /locus_tag="ACHKFK_000864"
     CDS             complement(6088..6738)
                     /gene="tetR(A)"
                     /locus_tag="ACHKFK_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO9051270.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPSADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            6844..8043
                     /gene="tet(A)"
                     /locus_tag="ACHKFK_000865"
     CDS             6844..8043
                     /gene="tet(A)"
                     /locus_tag="ACHKFK_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO9051271.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(8075..8959)
                     /locus_tag="ACHKFK_000866"
     CDS             complement(8075..8959)
                     /locus_tag="ACHKFK_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO9051272.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(9097..9489)
                     /locus_tag="ACHKFK_000867"
     CDS             complement(9097..9489)
                     /locus_tag="ACHKFK_000867"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFO9051273.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            9493..11244
                     /locus_tag="ACHKFK_000868"
                     /pseudo
     CDS             9493..11244
                     /locus_tag="ACHKFK_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            11281..11535
                     /locus_tag="ACHKFK_000869"
     CDS             11281..11535
                     /locus_tag="ACHKFK_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051274.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDSQSARCLNELRASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
     gene            11535..12125
                     /locus_tag="ACHKFK_000870"
     CDS             11535..12125
                     /locus_tag="ACHKFK_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051275.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRLEGDPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            12338..13390
                     /locus_tag="ACHKFK_000871"
     CDS             12338..13390
                     /locus_tag="ACHKFK_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051276.1"
                     /translation="MKDTITPLNYTKEAIKDLSVMEKGLRSNAPLTSYIMRQVIDMLD
                     SSVKFILPNCCDIIEPEEYRQTHFDLARLPYPVVTFEIPWFKDNVETQIGDFNISPSS
                     RRIALCWEARQSFEPIPGCNSILNTYIDGGVFILPVSWSDDLKIWILGVGGMFFPYNN
                     KLTKYEPDRTLPASRLVIDTLKENGVAKYNAAHFKAEPFITSMEFKDDLIKQVGSIER
                     LYAQIIMDTRDELQAFIQACSVLNCENVCPVTLSTKPERKFINGRKVQPPEKNKRPSY
                     TYKVLQLSETKVQSNHTGTGKSGGTKRMHLRRGHIRRKNNKLIWIRPAMINANSRAGI
                     VDKDYQINIRKEENKP"
     gene            13566..14168
                     /locus_tag="ACHKFK_000872"
     CDS             13566..14168
                     /locus_tag="ACHKFK_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO9051277.1"
                     /translation="MGKYQEERKTPVIVVKKRRTFSPPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            14196..14414
                     /locus_tag="ACHKFK_000873"
                     /pseudo
     CDS             14196..14414
                     /locus_tag="ACHKFK_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031752.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            14414..14680
                     /locus_tag="ACHKFK_000874"
     CDS             14414..14680
                     /locus_tag="ACHKFK_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051278.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNKANRGWRGFILRLIGVNLPDKRTEFTQRLLLTRKISGSVMKK"
     gene            complement(14949..15146)
                     /locus_tag="ACHKFK_000875"
                     /pseudo
     CDS             complement(14949..15146)
                     /locus_tag="ACHKFK_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15252..15527)
                     /locus_tag="ACHKFK_000876"
     CDS             complement(15252..15527)
                     /locus_tag="ACHKFK_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9051279.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(15527..15811)
                     /locus_tag="ACHKFK_000877"
     CDS             complement(15527..15811)
                     /locus_tag="ACHKFK_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO9051280.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWIIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(16710..17588)
                     /gene="repA"
                     /locus_tag="ACHKFK_000878"
     CDS             complement(16710..17588)
                     /gene="repA"
                     /locus_tag="ACHKFK_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO9051281.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASPLV"
     gene            18291..18932
                     /locus_tag="ACHKFK_000879"
     CDS             18291..18932
                     /locus_tag="ACHKFK_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO9051282.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            19073..19735
                     /locus_tag="ACHKFK_000880"
     CDS             19073..19735
                     /locus_tag="ACHKFK_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO9051283.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            20002..20622
                     /locus_tag="ACHKFK_000881"
     CDS             20002..20622
                     /locus_tag="ACHKFK_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO9051284.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            20649..22343
                     /locus_tag="ACHKFK_000882"
     CDS             20649..22343
                     /locus_tag="ACHKFK_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO9051285.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            22424..22666
                     /locus_tag="ACHKFK_000883"
     CDS             22424..22666
                     /locus_tag="ACHKFK_000883"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO9051286.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            22782..23117
                     /locus_tag="ACHKFK_000884"
     CDS             22782..23117
                     /locus_tag="ACHKFK_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051287.1"
                     /translation="MRTITTREQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFTIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GHVSITGKK"
     gene            23344..24414
                     /locus_tag="ACHKFK_000885"
     CDS             23344..24414
                     /locus_tag="ACHKFK_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO9051288.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSAATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            24418..24855
                     /gene="pilM"
                     /locus_tag="ACHKFK_000886"
     CDS             24418..24855
                     /gene="pilM"
                     /locus_tag="ACHKFK_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO9051289.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            24887..26506
                     /locus_tag="ACHKFK_000887"
     CDS             24887..26506
                     /locus_tag="ACHKFK_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO9051290.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISENCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            26527..27822
                     /gene="pilO2"
                     /locus_tag="ACHKFK_000888"
     CDS             26527..27822
                     /gene="pilO2"
                     /locus_tag="ACHKFK_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO9051291.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTLPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            27812..28270
                     /gene="pilP"
                     /locus_tag="ACHKFK_000889"
     CDS             27812..28270
                     /gene="pilP"
                     /locus_tag="ACHKFK_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO9051292.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            28374..29882
                     /locus_tag="ACHKFK_000890"
     CDS             28374..29882
                     /locus_tag="ACHKFK_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO9051293.1"
                     /translation="MLAPADQPPDDILEYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            29884..30978
                     /locus_tag="ACHKFK_000891"
     CDS             29884..30978
                     /locus_tag="ACHKFK_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO9051294.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            31040..31576
                     /locus_tag="ACHKFK_000892"
     CDS             31040..31576
                     /locus_tag="ACHKFK_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO9051295.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVTIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            31621..32106
                     /locus_tag="ACHKFK_000893"
     CDS             31621..32106
                     /locus_tag="ACHKFK_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO9051296.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            32122..32748
                     /locus_tag="ACHKFK_000894"
     CDS             32122..32748
                     /locus_tag="ACHKFK_000894"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO9051297.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            32766..34127
                     /gene="pilV"
                     /locus_tag="ACHKFK_000895"
     CDS             32766..34127
                     /gene="pilV"
                     /locus_tag="ACHKFK_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO9051298.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            34241..34435
                     /locus_tag="ACHKFK_000896"
                     /pseudo
     CDS             34241..34435
                     /locus_tag="ACHKFK_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            34600..35421
                     /locus_tag="ACHKFK_000897"
     CDS             34600..35421
                     /locus_tag="ACHKFK_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO9051299.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            35523..36725
                     /locus_tag="ACHKFK_000898"
     CDS             35523..36725
                     /locus_tag="ACHKFK_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO9051300.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            36829..37287
                     /locus_tag="ACHKFK_000899"
     CDS             36829..37287
                     /locus_tag="ACHKFK_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO9051301.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            37284..38120
                     /locus_tag="ACHKFK_000900"
     CDS             37284..38120
                     /locus_tag="ACHKFK_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO9051302.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            38104..39252
                     /gene="traJ"
                     /locus_tag="ACHKFK_000901"
     CDS             38104..39252
                     /gene="traJ"
                     /locus_tag="ACHKFK_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO9051303.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            39249..39539
                     /gene="icmT"
                     /locus_tag="ACHKFK_000902"
     CDS             39249..39539
                     /gene="icmT"
                     /locus_tag="ACHKFK_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HFO9051304.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            39603..43664
                     /locus_tag="ACHKFK_000903"
     CDS             39603..43664
                     /locus_tag="ACHKFK_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO9051305.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            43681..44031
                     /locus_tag="ACHKFK_000904"
     CDS             43681..44031
                     /locus_tag="ACHKFK_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO9051306.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAETGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            44043..44738
                     /locus_tag="ACHKFK_000905"
     CDS             44043..44738
                     /locus_tag="ACHKFK_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO9051307.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            44749..45723
                     /locus_tag="ACHKFK_000906"
     CDS             44749..45723
                     /locus_tag="ACHKFK_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO9051308.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            45727..47064
                     /gene="traO"
                     /locus_tag="ACHKFK_000907"
     CDS             45727..47064
                     /gene="traO"
                     /locus_tag="ACHKFK_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO9051309.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            47061..47774
                     /gene="traP"
                     /locus_tag="ACHKFK_000908"
     CDS             47061..47774
                     /gene="traP"
                     /locus_tag="ACHKFK_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO9051310.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            47771..48301
                     /gene="traQ"
                     /locus_tag="ACHKFK_000909"
     CDS             47771..48301
                     /gene="traQ"
                     /locus_tag="ACHKFK_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO9051311.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAA
                     AGAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVF
                     NDRVIDATLASLGIAF"
     gene            48348..48746
                     /locus_tag="ACHKFK_000910"
     CDS             48348..48746
                     /locus_tag="ACHKFK_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO9051312.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            48803..49054
                     /locus_tag="ACHKFK_000911"
     CDS             48803..49054
                     /locus_tag="ACHKFK_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO9051313.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            48969..49787
                     /locus_tag="ACHKFK_000912"
     CDS             48969..49787
                     /locus_tag="ACHKFK_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO9051314.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            49842..50201
                     /locus_tag="ACHKFK_000913"
     CDS             49842..50201
                     /locus_tag="ACHKFK_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051315.1"
                     /translation="MDTLTAGYQFSADAFRRFIIRTLTDDIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHNSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            50201..50581
                     /locus_tag="ACHKFK_000914"
     CDS             50201..50581
                     /locus_tag="ACHKFK_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051316.1"
                     /translation="MKIHRISPETLITLIHAHLAGKADSTAKEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(50554..51099)
                     /locus_tag="ACHKFK_000915"
     CDS             complement(50554..51099)
                     /locus_tag="ACHKFK_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051317.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTDAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIAYLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            51324..54368
                     /locus_tag="ACHKFK_000916"
     CDS             51324..54368
                     /locus_tag="ACHKFK_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9051318.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            54368..54988
                     /locus_tag="ACHKFK_000917"
     CDS             54368..54988
                     /locus_tag="ACHKFK_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO9051319.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            54946..56151
                     /gene="traW"
                     /locus_tag="ACHKFK_000918"
     CDS             54946..56151
                     /gene="traW"
                     /locus_tag="ACHKFK_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO9051320.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            56148..56717
                     /gene="traX"
                     /locus_tag="ACHKFK_000919"
     CDS             56148..56717
                     /gene="traX"
                     /locus_tag="ACHKFK_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO9051321.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            56792..58957
                     /locus_tag="ACHKFK_000920"
     CDS             56792..58957
                     /locus_tag="ACHKFK_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO9051322.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTTTSASEYHASNTQNPQSALLEFIGPGLTRFSNKVATIHSGTGTT
                     SQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREDRLKNINRDDKDGIKQ"
     gene            59046..59696
                     /gene="excA"
                     /locus_tag="ACHKFK_000921"
     CDS             59046..59696
                     /gene="excA"
                     /locus_tag="ACHKFK_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO9051323.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSISLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            complement(59835..59915)
                     /locus_tag="ACHKFK_000922"
                     /pseudo
     CDS             complement(59835..59915)
                     /locus_tag="ACHKFK_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
     gene            60245..60487
                     /locus_tag="ACHKFK_000923"
     CDS             60245..60487
                     /locus_tag="ACHKFK_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051324.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALTMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAEKPGKNIH"
     gene            60778..61146
                     /locus_tag="ACHKFK_000924"
     CDS             60778..61146
                     /locus_tag="ACHKFK_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051325.1"
                     /translation="MKDNNTTATPETWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLETTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            61150..61365
                     /locus_tag="ACHKFK_000925"
     CDS             61150..61365
                     /locus_tag="ACHKFK_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051326.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(61563..62186)
                     /locus_tag="ACHKFK_000926"
     CDS             complement(61563..62186)
                     /locus_tag="ACHKFK_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9051327.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRADSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRVVSGYSL"
     gene            62346..62813
                     /locus_tag="ACHKFK_000927"
     CDS             62346..62813
                     /locus_tag="ACHKFK_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO9051328.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            62963..63139
                     /locus_tag="ACHKFK_000928"
     CDS             62963..63139
                     /locus_tag="ACHKFK_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051329.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(63202..63297)
                     /gene="dqlB"
                     /locus_tag="ACHKFK_000929"
     CDS             complement(63202..63297)
                     /gene="dqlB"
                     /locus_tag="ACHKFK_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO9051330.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVD"
     gene            complement(63886..63981)
                     /gene="dqlB"
                     /locus_tag="ACHKFK_000930"
     CDS             complement(63886..63981)
                     /gene="dqlB"
                     /locus_tag="ACHKFK_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO9051331.1"
                     /translation="MINLLIIVLRAVVAVANAVIAILELIRELVD"
     gene            complement(64034..64180)
                     /locus_tag="ACHKFK_000931"
     CDS             complement(64034..64180)
                     /locus_tag="ACHKFK_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051332.1"
                     /translation="MSGDLPSHQSIGTMDLCYSKTWPDKQVVLRLVLWPEEVSFQLTM
                     KELL"
     gene            64518..>65731
                     /locus_tag="ACHKFK_000932"
     CDS             64518..>65731
                     /locus_tag="ACHKFK_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HFO9051333.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFGQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEVFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTG"
BASE COUNT        16087 a        18251 c        17022 g        14371 t
ORIGIN      
        1 ggcacccccc ttcggcgtgc gcggtcacgc gcacagggcg cagccctggt taaaaacaag
       61 gtttataaat aggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
      121 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
      181 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
      241 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
      301 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
      361 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
      421 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
      481 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctgcaggc
      541 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
      601 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
      661 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
      721 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
      781 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaacacgg
      841 gataataccg caccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
      901 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
      961 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     1021 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     1081 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     1141 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     1201 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     1261 atcacgaggc cctttcgtct tcaagaattt tataaaccgt ggagcgggca atactgagct
     1321 gatgagcaat ttccgttgca ccagtgccct tctgatgaag cgtcagcacg acgttcctgt
     1381 ccacggtacg cctgcggcca aatttgattc ctttcagctt tgcttcctgt cggccctcat
     1441 tcgtgcgctc taggatcctc cggcgttcag cctgtgccac agccgacagg atggtgacca
     1501 ccatttgccc catatcaccg tcggtactga tcccgtcatc aatgaaccgg actgccacgc
     1561 cctgagcgtc aaattccttt atcagttgga tcatatcggc agtgtcgcgg ccaagacggt
     1621 cgagcttctt aaccagaatg acatcacctt cctccacctt catcctcagc aaatccagcc
     1681 cttcccgatc tgttgaactg ccggatgcct tatcggtaaa tatacggttt gctttcacac
     1741 ctgcgtcttt gagtgctctg acctgaagat caagagactg ctgactggtt gagacccgag
     1801 cgtaaccaaa aagtcgcata aaaatgtacc ttaaatcgaa tatcggacaa ctcatgtcta
     1861 ttattacaaa tttacgattt aatagacata ttaatgtaac agttttacga tgtccgataa
     1921 tttataacat ttcgtacggt tgcaaaaatg ttactaaatg cccgtcaggc agggaggccg
     1981 atatgcccgt tgactttctg accactgagc agactgaaag ctatggcaga ttcaccggtg
     2041 aaccggatga gcttcagctg gcacgatatt ttcaccttga tgaagcagac aaggaattta
     2101 tcggaaaaag cagaggtgat cacaaccgtc tgggcattgc cctgcaaatt ggatgtgtcc
     2161 gttttctggg caccttcctc accgatatga atcatattcc ttccggcgtc cggcatttta
     2221 ccgccagaca gctcgggatt cgtgatatca ccgttcttgc agaatacggt cagagggaaa
     2281 atacccgccg tgagcatgca gcgctgatac gtcagcacta tcagtatcgt gaatttgcct
     2341 ggccctggac atttcgcctt acccgtcttt tatatacccg gagctggata agcaacgaac
     2401 gtcctggcct gcttttcgat ctggcgacag ggtggcttat gcaacatcgt attattctcc
     2461 ccggagccac tacgctgacc cggttgattt cagaggtaag ggaaaaggcg acgttgcgcc
     2521 tgtggaacaa actggcactg ataccgtcag ccgaacagcg ttcacagctg gagatgctgc
     2581 tggggccaac tgattgcagc cgcctgtctt tactggaatc actgaaaaag ggccctgtga
     2641 ccatcagtgg tccggcgttt aatgaagcaa ttgaacgctg gaaaactctg aacgattttg
     2701 gcctgcatgc tgaaaacctg agtacactcc cggctgtgcg cctgaaaaat ctcgcacgtt
     2761 atgctggtat gacttcggtg ttcaatattg ccaggatgtc accgcagaaa aggatggcgg
     2821 ttctggttgc ctttgtcctt gcatgggaaa cgctggcgct ggatgatgca ttggacgttc
     2881 tggacgccat gctggccgtt atcatccgtg acgccagaaa gattgggcag aaaaaacggc
     2941 tccgctcgct gaaggatctg gataaatctg cattggcgct cgccagcgca tgttcgtacc
     3001 tgctgaaaga agaaacaccg gacgaatcga ttcgtgctga ggtgttcagc tacatcccaa
     3061 ggcaaaagct ggctgaaatc atcacgcttg tccgtgaaat tgcccggccc tcagacgata
     3121 attttcatga agaaatggtg gagcagtacg ggcgcgttcg tcgtttcctg ccccatctgc
     3181 tgaataccgt taaattttca tccgcacctg ccggggttac cactctgaat gcctgtgact
     3241 acctcagccg ggagttcagc tcacggcggc agttttttga cgacgcacca acggaaatta
     3301 tcagtcggtc atggaaacgg ctggtgatta acaaggaaaa acatatcacc cgcaggggat
     3361 acacgctctg ctttctcagt aaactgcagg atagtctgag gcggagggat gtctacgtta
     3421 ccggcagtaa ccggtgggga gatcctcgtg caagattact acagggtgct gactggcagg
     3481 caaaccggat taaggtttat cgttctttgg ggcacccgac agacccgcag gaagcaataa
     3541 aatctctggg tcatcagctt gatagtcgtt acagacaggt tgctgcacgt ctttgcgaaa
     3601 atgaggctgt cgaactcgat gtttctggcc cgaagccccg gttgacaatt tctcccctcg
     3661 ccagtcttga tgagccggac agtctgaaac gactgagcaa aatgatcagt gatctactcc
     3721 ctccggtgga tttaacggag ttgctgctcg aaattaacgc ccataccgga tttgctgatg
     3781 agtttttcca tgctagtgaa gccagtgcca gagttgatga tctgcccgtc agcatcagcg
     3841 ccgtgctgat ggctgaagcc tgcaatatcg gtctggaacc actgatcaga tcaaatgttc
     3901 ctgcactgac ccgacaccgg ctgaactgga caaaagcgaa ctatctgcgg gctgaaacta
     3961 tcaccagcgc taatgccaga ctggttgatt ttcaggcaac gctgccactg gcacagatat
     4021 ggggtggagg agaagtggca tctgcagatg gaatgcgctt tgttacgcca gtcagaacaa
     4081 tcaatgccgg accgaaccgc aaatactttg gtaataacag agggatcacc tggtacaact
     4141 ttgtgtccga tcagtattcc ggctttcatg gcatcgttat accggggacg ctgagggact
     4201 ctatctttgt gctggaaggt cttctggaac aggagaccgg gctgaatcca accgaaatta
     4261 tgaccgatac agcaggtgcc agcgaacttg tctttggcct tttctggctg ctgggatacc
     4321 agttttctcc acgcctggct gaggggtttg aagcccaatg gagcggaaac gtacgctaag
     4381 aagctaacta tatgatttta agtcaataaa gtcgtggagt tgttaggcag gtttacgttg
     4441 atttgattac cgagtttatt aacactgaaa gtccctgaaa ccgcatggct actggcattg
     4501 acgggaagag taaacaaaac cctcgttttc tttactcacc tcttctgctc aaaaaatgaa
     4561 cttagcgtac gatctcgtac ggttgggcct caaaccccgc tgatgccggt gcttcggttt
     4621 tctggcgaat ggaccatgat gccgactatg gcgtgctgaa tgatattgcc agagggcaat
     4681 cagatccccg aaaaatagtc cttcagtggg acgaaatgat ccggaccgct ggctccctga
     4741 agctgggcaa agtacaggtt tcagtgctgg tccgttcatt gctgaaaagt gaacgtcctt
     4801 ccggactgac tcaggcaatc attgaagtgg ggcgcatcaa caaaacgctg tatctgctta
     4861 attatattga tgatgaagat taccgccggc gcattctgac ccagcttaat cggggagaaa
     4921 gtcgccatgc cgttgccaga gccatctgtc acggtcaaaa aggtgagata agaaaacgat
     4981 ataccgacgg tcaggaagat caactgggca cactggggct ggtcactaac gccgtcgtgt
     5041 tatggaacac tatttatatg caggcagccc tggatcatct ccgggcgcag ggtgaaacac
     5101 tgaatgatga agatatcgca cgcctctccc cgctttgcca cggacatatc aatatgctcg
     5161 gccattattc cttcacgctg gcagaactgg tgaccaaagg acatctgaga ccattaaaag
     5221 aggcgtcaga ggcagaaaac gttgcttaac gtgagttttc gttccactga gcgtcagacc
     5281 ccaaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg
     5341 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc
     5401 gcccctcatc tgtcagcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca
     5461 gtgagggcca agttttccgc gaggtatcca caacgccggc ggccgcggtg tctcgcacac
     5521 ggcttcgacg gcgtttctgg cgcgtttgca gggccataga cggccgccag cccagcggcg
     5581 agggcaacca gcccggtgag cgtcggaaag gcgctggaag ccccgtagcg acgcggagag
     5641 gggcgagaca agccaagggc gcaggctcga tgcgcagcac gacatagccg gttctcgcaa
     5701 ggacgagaat ttccctgcgg tgcccctcaa gtgtcaatga aagtttccaa cgcgagccat
     5761 tcgcgagagc cttgagtcca cgctagatct atctcatctg cgcaaggcag aacgtgaaga
     5821 cggccgccct ggacctcgcc cgcgagcgcc aggcgcacga ggccggcgcg cggacccgcg
     5881 ccacggccca cgagcggacg ccgcagcagg agcgccagaa ggccgccaga gaggccgagc
     5941 gcggccgtga ggcttggacg ctagggcagg gcatgaaaaa gcccgtagcg ggctgctacg
     6001 ggcgtctgac gcggtggaaa gggggagggg atgttgtcta catggctctg ctgtagtgag
     6061 tgggttgcgc tccggcagcg gtcctgatca atcgtcaccc tttctcggtc cttcaacgtt
     6121 cctgacaacg agcctccttt tcgccaatcc atcgacaatc accgcgagtc cctgctcgaa
     6181 cgctgcgtcc ggaccggctt cgtcgaaggc gtctatcgcg gcccgcaaca gcggcgagag
     6241 cggagcctgt tcaacggtgc cgccgcgctc gccggcatcg ctgtcgccgg cctgctcctc
     6301 aagcacggcc ccaacagtga agtagctgat tgtcatcagc gcattgacgg cgtccccggc
     6361 cgaaaaaccc gcctcgcaga ggaagcgaag ctgcgcgtcg gccgtttcca tctgcggtgc
     6421 gcccggtcgc gtgccggcat ggatgcgcgc gccatcgcgg taggcgagca gcgcctgcct
     6481 gaagctgcgg gcattcccga tcagaaatga gcgccagtcg tcgtcggcac tcggcaccga
     6541 atgcgtatga ttctccgcca gcatggcttc ggccagtgcg tcgagcagcg cccgcttgtt
     6601 cctgaagtgc cagtaaagcg ccggctgctg aacccccaac cgttccgcca gtttgcgtgt
     6661 cgtcagaccg tctacgccga cctcgttcaa caggtccagg gcggcacgga tcactgtatt
     6721 cggctgcaac tttgtcatgc ttgacacttt atcactgata aacataatat gtccaccaac
     6781 ttatcagtga taaagaatcc gcgcgttcaa tcggaccagc ggaggctggt ccggaggcca
     6841 gacgtgaaac ccaacagacc cctgatcgta attctgagca ctgtcgcgct cgacgctgtc
     6901 ggcatcggcc tgattatgcc ggtgctgccg ggcctcctgc gcgatctggt tcactcgaac
     6961 gacgtcaccg cccactatgg cattctgctg gcgctgtatg cgttgatgca atttgcctgc
     7021 gcacctgtgc tgggcgcgct gtcggatcgt ttcgggcggc ggccggtctt gctcgtctcg
     7081 ctggccggcg ctgctgtcga ctacgccatc atggcgacgg cgcctttcct ttgggttctc
     7141 tatatcgggc ggatcgtggc cggcatcacc ggggcgactg gggcggtagc cggcgcttat
     7201 attgccgata tcactgatgg cgatgagcgc gcgcggcact tcggcttcat gagcgcctgt
     7261 ttcgggttcg ggatggtcgc gggacctgtg ctcggtgggc tgatgggcgg tttctccccc
     7321 cacgctccgt tcttcgccgc ggcagccttg aacggcctca atttcctgac gggctgtttc
     7381 cttttgccgg agtcgcacaa aggcgaacgc cggccgttac gccgggaggc tctcaacccg
     7441 ctcgcttcgt tccggtgggc ccggggcatg accgtcgtcg ccgccctgat ggcggtcttc
     7501 ttcatcatgc aacttgtcgg acaggtgccg gccgcgcttt gggtcatttt cggcgaggat
     7561 cgctttcact gggacgcgac cacgatcggc atttcgcttg ccgcatttgg cattctgcat
     7621 tcactcgccc aggcaatgat caccggccct gtagccgccc ggctcggcga aaggcgggca
     7681 ctcatgctcg gaatgattgc cgacggcaca ggctacatcc tgcttgcctt cgcgacacgg
     7741 ggatggatgg cgttcccgat catggtcctg cttgcttcgg gtggcatcgg aatgccggcg
     7801 ctgcaagcaa tgttgtccag gcaggtggat gaggaacgtc aggggcagct gcaaggctca
     7861 ctggcggcgc tcaccagcct gacctcgatc gtcggacccc tcctcttcac ggcgatctat
     7921 gcggcttcta taacaacgtg gaacgggtgg gcatggattg caggcgctgc cctctacttg
     7981 ctctgcctgc cggcgctgcg tcgcgggctt tggagcggcg cagggcaacg agccgatcgc
     8041 tgatcgtgga aacgataggc ctatgccatg cgggtcaagg cgacttccgg caagctatac
     8101 gcgccctagg agtgcggttg gaacgttggc ccagccagat actcccgatc acgagcagga
     8161 cgccgatgat ttgaagcgca ctcagcgtct gatccaagaa caaccatcct agcaacacgg
     8221 cggtccccgg gctgagaaag cccagtaagg aaacaactgt aggttcgagt cgcgagatcc
     8281 cccggaacca aaggaagtag gttaaacccg ctccgatcag gccgagccac gccaggccga
     8341 gaacattggt tcctgtaggc atcgggattg gcggatcaaa cactaaagct actggaacga
     8401 gcagaagtcc tccggccgcc agttgccagg cggtaaaggt gagcagaggc acgggaggtt
     8461 gccacttgcg ggtcagcacg gttccgaacg ccatggaaac cgcccccgcc aggcccgctg
     8521 cgacgccgac aggatctagc gctgcgtttg gtgtcaacac caacagcgcc acgcccgcag
     8581 ttccgcaaat agcccccagg accgccatca atcgtatcgg gctacctagc agagcggcag
     8641 agatgaacac gaccatcagc ggctgcacag cgcctaccgt cgccgcgacc ccgcccggca
     8701 ggcggtagac cgaaataaac aacaagctcc agaatagcga aatattaagt gcgccgagga
     8761 tgaagatgcg catccaccag attcccgttg gaatctgtcg gacgatcatc acgagcaata
     8821 aacccgccgg caacgcccgc agcatcgcga ccgtcatcgg tgagaagttc ggcaggtatt
     8881 gggtggtgac aatgtaggtg ctgccccaaa tggcaggtgc tatcgctgtg aacaataaat
     8941 cgggcgtgcg taatgacatg cgattacaag acctccgcta cggcgatggg tttttgagag
     9001 ttgcaggcgc cgcgatgacc gcaccctgac ttggactggc cctgcctcgc accaatccac
     9061 ctgcggctat ggccgcgatc atgcgaggcc cgaaaattac acgacctgta cacgtccgcc
     9121 gaacccggaa cgcaacagag cttcgtgggc atcccgatcc gggtcgtagc agcagtcctg
     9181 gaccaaacgc acgtcgtagt ccgcatcact agcccaggcg acgcttgaaa gaacaacgcc
     9241 ggtggtgctt atcccggcca tgacaagcgt gcttacgccg cgcgtccgaa ggtcggcgtc
     9301 gagcgccgtg ccataaaaga cgctggctcg cggacatgca tagaaaaggt cgccccgttc
     9361 gatcgcaagc ccttcgacgg gcagaccggt gcgaaatcgt ccgctcggga gatacggtga
     9421 gatttggcga ttcgtcgccg gcggcgcatg ttcatactct tcgccgagcg aaaagttggg
     9481 gaacagcacg ggccggccgc gcaaggcgtg ctggcagccg tgcagaccct gcgtgagatg
     9541 aacgccgaca acctgcgcaa ggtgccggcc gatgcaccca cggccttcat caagccgcgc
     9601 tggaagccgc tggtgatcac cccggaaggc ctcgaccgga aattctacga aatctgcgcc
     9661 ctgtccgagc tgaagaacgc cctgcgctcc ggcgacatct gggtcaaggg ctcgcggcag
     9721 ttccgcgact tcgacgacta cctgctgccg gccgagaagt tcgccgcact caagcgcgag
     9781 caggccctgc ccctggcgat caacccgaac agcgaccagt acctggaaga gcgtttgcag
     9841 ctgctggacg agcagttggc caccgtcacc cgcctggcca aggacaacga gctgcccgat
     9901 gccatcctca ccgagtcagg gctgaaaatc accccgctgg atgcggcggt gccggatcgg
     9961 gcgcaggcgc tgatcgacca aaccagccag ttactgccgc gcatcaagat caccgaactg
    10021 ctgatggacg tggacgactg gacgggcttc agccgccact tcacccactt gaaggacggg
    10081 gccgaggcca aagacaggac gttgctgctg tccgcaatcc tcggtgatgc gatcaacctc
    10141 gggctgacca agatggccga gtcgagcccc ggcctgacct acgccaagct gtcctggctg
    10201 caagcctggc acatccgcga cgaaacctat tcggcggcct tggccgagct ggtcaaccac
    10261 cagtatcgcc acgcctttgc cgcccactgg ggcgacggca cgacctcatc ctccgatggc
    10321 cagcgcttcc gcgcgggtgg ccggggcgag agcaccgggc acgtcaaccc gaagtacggt
    10381 agcgagccgg gacggctgtt ctatacccat atctccgacc agtacgcgcc gttcagcacc
    10441 cgcgtggtga atgtcggcgt ccgcgattcc acctatgtgc tcgacggcct gctgtaccac
    10501 gagtccgacc tgcggatcga ggagcactac accgacacgg ccggcttcac cgatcacgtc
    10561 tttgccctga tgcacctgct aggcttccgc ttcgcgccgc gcatccgcga cctcggcgaa
    10621 accaagctgt acgtgccgca gggcgtgcaa gcctacccga cgttgcgccc gctgatcggc
    10681 ggcaccctga acatcaagca cgtgcgtgcc cactgggacg acatcctgcg cctggccagc
    10741 tcgatcaagc agggcaccgt caccgcctcg ctgatgctgc gcaagctcgg cagctacccg
    10801 cgccagaacg gactggccgt ggccctgcgc gagctgggcc ggatcgagcg cacgctgttc
    10861 atcctggact ggctgcaaag tgttgaactg cgccgccgcg tgcatgccgg cctgaacaaa
    10921 ggtgaggcgc gcaactcgct ggccagggcg gtgttcttca accgccttgg ggaaatcagg
    10981 gatcggagct tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggct
    11041 atcgtgctgt ggaacacggt gtacctggaa cgcgccaccc aggggttggt cgaggccggc
    11101 aagccggtgg acggcgagct gctgcaattc ctgtcgccgc tgggctggga gcacatcaac
    11161 ctaaccggcg attacgtctg gcggcagagc cgcagactgg aagacgggaa gtttcggccc
    11221 ttacggatgc ccggaaaacc ttagcgtacg attttttccg aattctgcgg gctccccagg
    11281 atggatgaag aaagggtgtt ttcactcagc tatgagcagt tgacccgaat agcggaaaaa
    11341 aatatccgtg aatgtgggct ggacagccag agcgccaggt gcctcaatga actgagggcc
    11401 tcagctctgc tgtggttgtg gtatgaactg gccattcatg gtgcgccaca gaacaatcac
    11461 gcacaggccc gggaacgcat cgataccgat tatcagcgcc tcaaaaaact catctggtcg
    11521 gagggtgaca gttaatgact cagagccgcc gaccgtcgcc actgcagcgg agagtgctga
    11581 ttgtgctggc tgcccttgat gaaaaacggc cgggacccgt gctgacgcgg gacatagaac
    11641 gggtgctgga gcgaagcggg gaagccccgg tctacgggcc aaacctgcgc gcctcctgcc
    11701 gtcgtctgga ggatgccggc tggctgcgta ccctgcgcgc tccgaacctg cagctggcag
    11761 tggagctgac ggacgccggc cgggccgtgg cacagcctct gctgctggca gaacaggacc
    11821 gcctccgggc agaacagcgt gcggcagaag tcgtcgtact gccgctggtc ccggcagcag
    11881 gtctgcccgc agacggcact tctgccactg atctggcggt ggagctgaac ggcataacgt
    11941 atcaggcctg ccgtggggat tttgtggtgc gtctcgacgg cagcacctgt ctgcagttat
    12001 ggaataaaga aggcagggtg gtacgcctgg agggcgatcc gctggaagtg gcgcagtggc
    12061 tgcaggcctg tcatgacgca ggaatagagg ttcgcgtaca gataaatgaa agtagcgtcc
    12121 cgtaaagggg gcctgagtaa agcggcgcgg atggctgatt atttccaaga ataatcgtca
    12181 gttcaggtat cgtggatacc tgtaacagcc tcctgtgtac gagctaaatc cacacatctt
    12241 ctgccgtagc caggaaccta ctggctgagt atttctggtg gcagagctac gtaagtagcc
    12301 gtacctgtat agtaatcaga tatccagaga aaacgtaatg aaagatacaa ttactccact
    12361 aaattacact aaggaagcca taaaagacct gtctgtcatg gaaaaaggat taaggtcaaa
    12421 tgcacctctt acatcatata ttatgcgtca ggtcattgat atgctggaca gtagtgttaa
    12481 gttcatctta ccgaactgct gcgatattat tgaaccagaa gaataccgcc agacacattt
    12541 cgatttggca cgattaccat atccagttgt aacatttgaa ataccatggt tcaaagacaa
    12601 tgtagaaacc caaataggtg attttaatat atcaccatca tccagaagaa ttgccctgtg
    12661 ctgggaggcc agacagagtt ttgaacccat cccgggatgt aattcaatcc ttaacactta
    12721 tattgatggc ggagtgttta tccttcctgt ttcatggtct gatgatttaa aaatatggat
    12781 tcttggtgtc ggtggaatgt ttttcccata taacaacaaa ctcactaaat acgaacctga
    12841 caggacattg cctgcatcca ggcttgtaat cgacacatta aaagagaatg gagtagccaa
    12901 atataatgca gcacacttca aagcagagcc ttttataaca agcatggaat ttaaagatga
    12961 tttgataaag caggtgggaa gtattgaaag attatatgcg caaattatta tggatactcg
    13021 cgatgaacta caggctttca tacaggcatg tagcgtgtta aattgcgaaa atgtgtgtcc
    13081 tgtcacactt agcactaaac cagaaagaaa attcataaat ggtcgtaaag ttcagccacc
    13141 tgaaaaaaac aagcgccctt catatactta taaagtattg caactgtctg aaactaaggt
    13201 gcaaagtaat catactggaa caggaaaatc cggtgggacg aaaaggatgc acttacgcag
    13261 aggacatatt cgcaggaaaa ataacaaact aatatggatc agaccagcaa tgattaatgc
    13321 aaacagtaga gcaggcattg ttgataagga ttaccagata aatataagaa aagaagaaaa
    13381 caaaccttag taaatcttac tgtgtaagta acaatccgtt gtatcctgct ataaacgttc
    13441 ctgattcaca tagcagggta cagagtcatc ttcgtaaatt atcaagggat attacataac
    13501 agaaagttta ttaaattttg aactatgata ttataaggaa gtagaacaac atagccgagg
    13561 tatttatggg aaaatatcag gaagaaagga aaactccggt cattgtggtg aaaaaacgtc
    13621 gtactttttc gccaccgtcc ttatccgaaa aaacagatat tatcgcacct gtatttactg
    13681 aacagacggc agaatcagcc cccgccggaa taaattcatc tgccgtcgaa acacatatcc
    13741 ccgaagcgcc agccaggaag aagaaaaaga aaagacaccg ttttccacgt ccctcccact
    13801 ggacccgtga gtatactcac gagtgcgtgg aaaaaataaa ggccctgttt cctcatcttc
    13861 gtgctgaagg tggtggattt attcccctga aaattggcat aaacaatgat atttcggcat
    13921 ttctggcaga acacccggag acagaactga ccatggacga atggctctgt gcagtgtcct
    13981 gcatcacatc ccgccgcgta tatctgcagc ggacagctgt cgccggcgtc ccacgttatg
    14041 gcctggatgg tcaccccaaa gggcaggtct cagactctga agcacagagt gccggtcgca
    14101 gactggcgac attagaacaa aagtggctca ggatgcaggc acagcaggag aatatttctg
    14161 ggcaataaac actcctgata acaggtggac attacgtatc caaagattct cctgatgcga
    14221 aacaggagct tcttgagtgt cttttgtccg gaaaaagaat gaccgggcgg gatgaaatgg
    14281 atatcaagcg cattctaaaa acaaaggaag cacttgataa catcaggaat tttattgata
    14341 aacagcagtt aacgaatagt gatactccga agaaaagcac ggtcccaaat gaagataaaa
    14401 aaggcataag atgatgatca caggaaatgg aataaacact gtaactgtca atggaaaagt
    14461 gaaacacatc acagaactgg attatataac actttgttta gagtggacaa aactacgaga
    14521 ggaaaacaat cgtctttatg aaattaataa caaggcaaac agaggatggc gtggtttcat
    14581 cctaagattg ataggggtta acctgccaga taaaaggact gagtttacac aacgtctctt
    14641 attgacgcgc aaaatttcgg gttcggtcat gaaaaagtaa catattctat gctggaacag
    14701 accaattttg cattcgccgt ccaggagttt gaggggaaag tagtcagtgt ttacgctcag
    14761 tcagggaaag tgtgcaatga taaacaacac gaaaggaatc gtagtacatg ggcaccgcga
    14821 ttcaatattc aaggaaaaca caatacagca atgtacttgt actgtttcta ttattctggc
    14881 gatttcaata aaggcacttt ctcgcgctct gtcattttct gaaactcttc atgctgcatt
    14941 tcgatttccc ttctaagatc ataactcgca tacctgttct ttttatgtat atctattttt
    15001 gtgacgagtt caaaccacaa tgttcgcact ccccaggcag cgttatgaaa catttccgca
    15061 acggctatag agtctgtatt aactgcttcg gtcatgtagt cgctaatacg ttcagcggta
    15121 attttcgtta tttcatcgta attcatttta tctcctacaa gattatttaa tggaatgcaa
    15181 aatatctggc ctgataataa tgttccagaa ttaatatctg caggctatgt taagttgtcc
    15241 ccacacacaa tctacctgtt ttctcgtgtg tgccacaggc gcaatacata aatagtctgg
    15301 ccagtaagtt cgtaacggat ttcgtactcg ccagcaaaaa tccgtctgac ttccctgggt
    15361 tcaaactgaa ataactgttc ccccatacgt ggatgagtta acagaatgac cggagcctgt
    15421 gtcagggact gcaccgttct ggcggccgca ggtttactgg ccagcaccag aaaatcatat
    15481 aaccgcgcca aatcagaaag cgccttactg gtccacttca gttccatcag cgtggcaccg
    15541 gtaacggatt gtcagtactg aggctgtccg cccaggcctg tacagcctga tggtcgataa
    15601 cctgtccgga tgtcacatcg tccagggctt ccagcgtcag gcgattacgc tcctcctcct
    15661 gggcaagcca tgcagaaagc gcctgtttga taatccagcc ccgggagcgt tccagtctgg
    15721 cggccatctg gtcgacttta tcagccatag gtaatggcac atgcgcggta atgacttttg
    15781 ttgcctgtgc ggtattgttt ttcatttgca tatccgatta gctgttaatc atatttaatc
    15841 atagtgatta ctttcagacg aaacaaggca aataatgcgc gtcagcgcat gtaaggcggc
    15901 actccgttgt tacatatggc acagaaacaa aaccgccgaa gcccggcgtc agccggtact
    15961 gattgataga tttcacctta cccatcccca gccctgctaa gccatacccg ttttcagcca
    16021 tgacagagct tctgtgcgcg gtcggagtgg tcccgacgag ggtttacccg aagtcggggc
    16081 gtatctccgc gttagcgggc cgtcagggcc gcttacgagc gtgtactgag gacttccagc
    16141 cagaagactg acagcgatga ggattcttag ttacaacatt cataattaaa agcgatgttg
    16201 ttcccgccct tcgggcgggg gcggcctcgc ttttcagtta tgaggggtag gcacggtgct
    16261 gcgtttgtgt ggtgttttta ttcctgatgt ctttttgttg tggtttcaga acggtgaggt
    16321 tcggggcccg cgcgtcagcg tgggaggtgc atatttgtct gtccacagga caggcactga
    16381 ataggtcttt tttttaaatg gaagtagtta agtagtttaa aggaaataca gtactggggt
    16441 ttaaaagaaa cattgcaccc tgtaacgcct gcggctggcg ctttatagca ttaacggttg
    16501 taaccttacg gagaagtccc ttgcagttaa atgtggataa acagaattcc ccgtctgtga
    16561 gacgtgtttt gcgtgaaaaa cagggggagc aaggcgagcc ggaaggcaga tgatgaggct
    16621 gttttttgtg tgctgtgctg cttttttgtg cgtcgccggg cttcagacgt gcggaagcct
    16681 ccggcgcagg ccggattatt cagatgaggt caaactaagg gtgaggcagt ggccagccgt
    16741 gtgtagttgt tgttccgcga cattatcatg cgttccttca cgcgacggtc gatttcgcgc
    16801 ttcacggcct ccaggcttcc ccggaaacgc ccttccgcga tttcacgtgt cagctgccgt
    16861 ttcaccaggg tgacaatgtc ctgacgggtc ctgcctgcat cgcggcgcgc acgggcacgt
    16921 ttcatgccgt gggacttaag ctctgcctga taactgcgga aacgctcgcg cacaaaacgc
    16981 caggccctcg ctatcagttc atccataccc agcgcatcca tcccctgctt tttgcgcagc
    17041 ctgttttccc attccacgcg gctgcggcgt gcggaagcca ctgcctcttc tgagatatcg
    17101 agtgagtcaa acagtgccgg cgtgaacgtg atgtcggtcg ggatgttaca gccaattgtc
    17161 gggtcgtatt ccgtctggta ggttatcagc cccagctcag acaggaactt cagggcacgg
    17221 gtggcacggg tgatggagag tttcccggcc tcagactccg tcgccagtcc gcactcaatg
    17281 gccagcgtgg tgatggagca ctgcacgcgg ttggccagcg ggtcatagtg gaaacacagc
    17341 ccctgcagca gcgcatcaat cgcccggcgg cgcagcaccg gcggcatacg ccggcgcagg
    17401 ccgcgcgaac gggcatacgc cacatgcgcg gcaaaatcaa aacgggaggt gaagcccacg
    17461 gccttttcca tcagtttttc gcagaacttc agcgttccgg catcttcacg gggtgtgaac
    17521 accggattcg ggttctttac ctgacggtat gtgctttcag taaatgaggt ctggttcagg
    17581 gccgccacaa gctgtcgctt atggcgggaa cagattgccg tgcgttaatg cactgcaaag
    17641 ggttgaatct ctgacacggt ttcatatata cttatcccgt attctgtgag gcccccatta
    17701 tctttctctg attccgccaa gtttcgagga aagatgatgg gggttttcct ttatggtctt
    17761 ctgaaacttt tttcagcctt ccggccccgg caaactgccg tcgtttctgc catacatcct
    17821 gaatgcctta tggcgcattc tgccgttttc tggctttccg gaccgcgagg atcccaaaag
    17881 ccactgcata acgatccgca atatacccct tttccaccgc tttgaaaagc cgacacgatt
    17941 tctcagagtt acagttgccc tctttaaccc cctaaaaagc gtcgtttttt tgaactctgt
    18001 caaaaaaatg aacaatttca caaacgatct tttatgctgc ttttttcttc ttttccagta
    18061 gaagagatcg ccgaattttt ttaaacgatc taatatcccg cacacaacat caaatttaga
    18121 ataatattct gtcttatcaa atataacgat ctattcaact gcttatcctc gctcttgcca
    18181 cagatacatc cttatgtaat atattttcca gccattaatc ctgtttgcag gagttgtcgg
    18241 ttttcgcccg cctcgaaacc gtccacaagg cttacaagag tgcgtaaatc atgtgcctgt
    18301 cagaactgca ggccaggctg aacgtgggtc agccgctgtt tccgacacat gctttatgta
    18361 ttcgccgttc acaacaacat cgaagccccc ctgcggggat cgattttcgc ggagcgttcc
    18421 ggattttgaa tatcagtgaa ttacatcaac gcaactggta tctggcacag tacatccctg
    18481 ccggtaaaaa ccgcgaacat ctcttttcct ggctttccga acaacacgtc cttccctgga
    18541 cacccctgat actgaaaaag gtccgccgca cggataaagt ctgtggttac cgtcgacata
    18601 tacacgccgt gtttcccggt tactttttcc tgaaagcaga ccccgaaagc cacagcttca
    18661 ctcaccttcg ccgtcacagt gcctttctgg acttcgtgaa aatggccggt gaaatcaaaa
    18721 ccgtcaggga ggatatcgtt cagtctttga tgaaagtgta cccggatcct gcgctcaatc
    18781 cggcagccag ggaggagctg gatgctgcct ccaccctctg gctgacaaaa gcgcgttacc
    18841 agtacctcct ccggcttgat gcccagcctc tgccggagtc gaggatcgcc ctgctcctgc
    18901 acctggtatc tgatgacgga gcgttgacat gaaaattgct gtcttaattt gacgactttt
    18961 cccggccttt atggccggga tttttttccc ttctgtttcc acaatgcccg gcaccggtga
    19021 cacctgaggg tgtacggaga gcggctgtcg ggcatttttt caggacgcct ttatgaccat
    19081 tctatcccag gaaaaccctg tcattctgtt actggagaat atgctcgaag cactctccac
    19141 cgccccggac aacataaaca acgaacggcg tcgccggcgt tacctgctga actggcttga
    19201 cactgcccgt cagatgcgcg agttcagagg aatggctgaa gagttcacta ccctgcgaaa
    19261 actgctggcc accaattcag acataccggt agcaaacacc ctgaaatcac tgctggaaag
    19321 tggcaataac gcagcattat acgacctgta ccgcttccgc tcggctcttg ccacgtgcag
    19381 gagtctggga tggcagattg gcacgtgcgc atggaatgac cagttactga gtgagacact
    19441 ttcccgcgca cagaccggga aacgtcatat cctgcagctg aacagcaccg gggacacctt
    19501 ttccaccacg ggcagaatga tcagaccggt ggcattttca ttaatccatc cggccaccat
    19561 caacacgtct gaggttgaag agatattccg ggatgaaggt tttatgctcg cacacgggcg
    19621 cgaatgcagt ctgaacggca gcggcagaaa tatgctgtca cgcattcttc atgtgggcca
    19681 ttcaggttta gcggaagagg aatggggcat ggacaacagt ctgcttttac actgaaatac
    19741 cgggacagcc acaacggcag ccactctccg ctgacttttt ttcgcaaatc gcggtttgct
    19801 gatgtggatg tcccattttt tattatccgt cctctccggt tgtcataaag cgcattccgg
    19861 cgtgaggctt ctgttgttat catcccctcc tgcagacatc cccttgtctt ctgatgactg
    19921 acgatatgct tcttctgaca attaaagaag cggaagcttc acgccataat gttgtttgac
    19981 tgacctgcca caggaggaaa aatgacactg acgttactta ccgcgtataa cgcaccctat
    20041 ctggcagccc tgacttttgt tctgcttact ggtatcgctg aacttatcgc cctgctctgc
    20101 ggacactccc tctcttcagc tatggataca cctgatttac cggagggtct gacaggagag
    20161 gcgctggact ggctgaacat cggacggatc cccctgctga tcgtcctctg tatgctggca
    20221 ggtttttttg gtatatccgg cattctcctg cagggtctta ttattcatct gctgcaggct
    20281 cctgctccga acattctcct tgcaccactt tgcctgctgc tgacatgtcc tctcgtacac
    20341 cgcaccggca gactgattgc cccctggtta ccccgtgatg aaacaacggc gctcaccgag
    20401 gaggcattca tcggctgtat ggccaccatc actggttatc acgcagtggc gggttccccc
    20461 tgtgaaggtc gcttcacaga ccgcttcggg caggttcatt acctgttgct ggagccggag
    20521 gccgggaaag aattcagacg aggagacaaa gtgattattg tctgccgtct ttccggttcc
    20581 cgctggctgg gcgaactgaa tccctggccg gatgttcttt aatcccgaca tatacagagg
    20641 ttctcactat gtttaatctt ccgggatggt tgtttaccgt tatagcgctg gtcctcaccc
    20701 tgttcgttat cgggctgata tttgcccgcc tgtaccgccg ggcatcggca gagcaggcct
    20761 ttgtccgcac aggtctggga ggccaaaaag tggtaatgag tggcggtgca attgttatgc
    20821 ccatttttca cgaaacaatt cccgtcaaca tgaacaccct gaagctggaa gtcagtcgtg
    20881 cagcggctga aagtctcata acccgcgacc gcatgcgtgt tgatgttgcc gttgcctttt
    20941 ttcttcgggt aaaaccatca gccgaaggaa tatccactgc agcccagacg ctgggacaac
    21001 gcacgctcac acctgaagat ctgcgtagtc tggtcgagga taaatttgtt gatgccctac
    21061 gggccacggc ggccagaatg agcatgcagg atttacagga tgcccgcgag aactttgttc
    21121 agggtgtgca gaacaccgtt gcggaagact tatccaaaaa cggtctggag cttgaaagtg
    21181 tgtcgctgac aagtttcaac cagactgcac gggtacattt taatcctgac aatgcctttg
    21241 atgctgaagg cctgaccctt ctgacccagg aaacagaacg tcgtcgccgc gaacgcaacg
    21301 aagttgagca ggatgttgaa gtggccatca gggaaaaaaa ccgggatgcc ctctcccgac
    21361 gtctggagat tgaacagcag gaagccttca tgactctgga gcaacaacaa cgggtaaaaa
    21421 cccgtacggc agagcagagt gccagtattg ctgctattga agctgaacga cgtcgtgaag
    21481 cagaatccgc gcgaattctg gctgaacgta agatagagga agccgaaatc gaacgacagc
    21541 agatagtgcg tacccgccag gtcgaagccg aacgggaagt ggccatacgg gaaattgagc
    21601 aacagcaggc aaccgaaatc gccagccagg ccagagccat tgccgtggcg gcaaaatcgg
    21661 aagagcagtc acaggcagaa gcccgggcaa gtaaagcact ggctgaggcc gtacaggccc
    21721 agcaggatgt ggaaaccacc cgccagactg cggaggccga ccgtaccaaa caggttgccc
    21781 tgattgctgc tgctcaggaa gctgaaacga aagcggttga actgacggtt cgcgcacagg
    21841 ctgaaaaaga agcagccgaa ctccaggcag cagcaattat tgaactggcg gaggccaccc
    21901 gtcagaaagg tctggcagaa gcagaagcac agcgcgcact gaacgatgcc attaacgtgc
    21961 tttcggatga gcagaccagc ctccgcttcc gtcttgccct gctggatgca ttacctgccg
    22021 tcatagagaa gtccacagaa ccaatgaaat ccattgacgg tattaaaatc attcaggtgg
    22081 atggtctgaa tggcggtgtt acccgcggta aaacagcaac aaccgacata agtggcggaa
    22141 atctcgctga acaggcagta accgccgctc tggcttaccg tacacaggct ccgctcatcg
    22201 actccctgct gaaagaggtg ggtttatccg gtgaaggact tacagccctg acacgtccac
    22261 tggtcatgcc tgccccggaa aaggttccgg atgtcagcga accggtaacc gctccggata
    22321 actcagaacc acaacaggtt taatccccgt gccggaattt ttccggcatc tttttcacaa
    22381 agaatgccct cacgggatat tctggtttaa aagaggattg aaaatgtccg cagaaaaatt
    22441 cagagttgtc atatacacca atacatgtga aaagaaagtc agttatattc ctgcaacaga
    22501 agatataaac atcgctataa tgccatttca gacggcagac aatggcttaa ttattgtgtg
    22561 tcagaactcc cgtataatac gacgcaaaga gcgtattccc ggaagcagct gtgaatggaa
    22621 agatgtgaca ttagcaccaa aatccagaaa ccgcgttcag ttatagcctg acagcctgct
    22681 tttcatcccc cataaaaggg ggtaaaaatt aaatatttca caagcgcgat atttttcagg
    22741 aatgcagatt attttctgtt atgaccaatt gagaaacagg catgagaaca atcacaacac
    22801 gtgaacaatt attaattaat gggagagtcc acgaaagaat cgccacacat atagttacag
    22861 gagctcatgg gtatgaaacg ctttgcacaa atggctataa cgtcagatac aacaaggcgc
    22921 agcaactggt tgaaagctgc gaaaaaatag ctgaaggtaa actacctgtt acctgtcctg
    22981 tctgtttcac catatggcag gatgtacaca gattcaccca cgttgacttt gacacacaat
    23041 cggggaaaag tgattttatt gatacctgcc attcggagat cataaccgga catgtttcaa
    23101 taacaggcaa gaaataaaat aatgttccag ttctgaaaaa caatatcact tgttttctgg
    23161 aaactatcat ttgcagcagg acgtttttct ttttatattt ccggaaccgg aatgttttag
    23221 ttttcataat caccccgaac atgaatgttc atcatcaata tcctgatgaa attatattta
    23281 tctgaagacc tgttaccagc cacctgtctt ataccgtaac ccgatacata ccggatttat
    23341 tctatgaaaa agaaatcact tacactcctt ctgccgtcat tactcagcgg ctgtacggca
    23401 tttaatgcca cgcctgttcc tgacaccacg cctccggctc tggtgttcgt cgacggacaa
    23461 attcgcgaaa gcgctgccat tatcgcccat acgcaaaaac gtctgcttcc gccaccggtg
    23521 acccgccctg tgacgccgcc ccgcccgaca acaacagcga cgcccacaac cgcagtcagc
    23581 agccagagta ccgcactgcc tccccgtccg ctccttcagg ccacacagct gcccgcactg
    23641 gttatgaccg gtaaacccgg tcagtcatcc gtcgtttatt tacgtgaggc acatcagcgc
    23701 tccgtcagtg actggatacg ccagttgcta ccacccggct ggcagttccg gcaacagaac
    23761 gctgccatac cggcactgaa caaacgcctc cccgtctggt ccgttaatga ccagtggcac
    23821 cgggcactga atgcgctgct ggccggccag cagctttacg gacatatgga ctggaacagg
    23881 aaaatactga ctgtcacgac ctctgcaaca ccgccggtcg acgtcacggc accgcagggt
    23941 tcacagaaag ctgctgatac accgcgtaat ccgttcaggg gcagcgccgc aacgccggca
    24001 ggcccgacac cagtgacacc tgccgtcagc gtttcccggg ctcagaccac caccgggaca
    24061 tccggaactc cagagagcag aacaaccacg ccagccccgg tggccacgaa accggtagtg
    24121 ccaccacagc agaaggtcat accgccacgg cagtggaaaa tggaaaaggg gaccaccctg
    24181 aaagacggac tggcccgctg gacatcatcc gaaaaatgcc cgcacggcac atggacactg
    24241 cgctgggaaa cacctgtcaa ctacagcatc gatgcccctc tggttttcac cggcagtttc
    24301 cgccaggcgc tggagcagct ctttgcactc tataccccgg ctgaaaaacc gctgtatgcc
    24361 cggaccagca ctgcacaatg cctgctccgt gtgacagacc gcccgcagga ataacctatg
    24421 ggatgggtta cgggtttcat tatggtcatt atggccattg gtaatttctg gttccacagt
    24481 cacctcagcc gtactgtgca ccatcagcag accgctgaaa tcacacagca ggctgcagac
    24541 tttattcgtt atatgaatgc catcaacgat tacctgtatc agcaccctga acgacgtgct
    24601 gccggtggtc agctcacgtc agcacagctc ggtctgcccg ccacgaaaaa cgtcagccac
    24661 ctcatcagtc agcagcgcgt ttttgtctgg gcaaaagaaa aacccggcct tatgggtgcc
    24721 ctcctggaac aaagtggtga ttcggccctg cttgccagag tggaaaacgg tcgcctcctg
    24781 gacacccgtg ggcggcgtat ttccatcacg ctccctgctg tgatccccga tcaggtcatc
    24841 atctggatga attaggccgg actctgaata actcacggat ttatctatga acaaaaaact
    24901 tcttgctatt atgattgcag ccactctgct gcagggctgt accagtttcc gacaggcaga
    24961 gaacaccaga aaagacgccg ttgctgaaca acaacgggcc cgggaaatga tcgctgagct
    25021 gcgaaaccgg acaccggttg taaaggaatc cccacgtcag tggatcaatc cccgtccggt
    25081 cacggataaa aaccccggta atgcggtgcc cggctgtccg attatcatta acacccggca
    25141 gagcatcacc cttcagcagg tggcccagcg gatctcggaa aactgtcata ttcctgtaag
    25201 gatcacccct gatgtacagg cgtatctgag cgtgaccacc tccggcagta cgcagcaaat
    25261 ccagggcaca gtaccaccgc cggataccgg cggtatggtt ccactggccg ccataggcag
    25321 ttctgacacg acacttcccc gcctgcaggg aaatacggcg tcacttcctg aactgaaggc
    25381 ttcaggactg acatcactgc tgaatgcggt cgcttcccgt ctcgggattt cctggcgtta
    25441 tgatggttca catatcacgt tctattatct tgatacccgg accttcccgg tgacctatat
    25501 ggacagccag gttgcctata atgccaccgt ggtcagcggt accatgtcat caaatggctc
    25561 atcaggtgga acagcatcgg atgccatttc cggtgatgcc agtaataccc agaccaccac
    25621 cgtggatatg aaatctgcgc tgtatcagga tgtgaaaaat gctgtctcat caatgctgac
    25681 gccgggtatt ggtcgcatgt tcatgtcgac gggctttatc accgtcacgg acaccccaca
    25741 ggtactggaa acggtccgga cgttcattga aaaacgcaat gaagaaatga agcgccaggt
    25801 ggtgctgaat gtggaaattc tgagtatccg gaaaacccgt aatgagcagg caggtatcga
    25861 ctggaacgca gtcttcagcg acagaacgct gggactgagc ctcggcagca cattcaccag
    25921 tgcagccagc gataccgtca ctggtggtgt ttccattgtg aacgggaaac tcaccggttc
    25981 aaaagccttc ctgaaagcac tctcttccca gggagatgtc agtgtggtaa cccggaattc
    26041 cgccgtgacc aaaaacctga ccccggtacc gatgcagatt gccaatcagc agagctacat
    26101 tgagagtgtg acgacggata ccaccgccaa tgtcggctca tccacctccc tgaatgcggc
    26161 caccatcacc accggtttta atatgaccct tctgccgttc attctcccgg actcacagac
    26221 actgcagctg ctctattcca tgagtctctc cgacaaaccg gttattgaga attacgagag
    26281 tggtggaagc aaagcccagc tgccgaatgt ggatctgaaa accatcaacc agacagtgga
    26341 cctgaaatcc gggcagacag tcattatcag tggcttccag cagtcagggc gtcgcagtgg
    26401 aaaacagggt gtcggcacac cgggattctt cggtcttggc ggtgggatca acagtgaaaa
    26461 cgacgacacc attctcgtgg tgctgatcac gcccaatatc atctgatttg cagaaggagg
    26521 actatcgtga cagatatttc accgacagag cgacaggtgt ttccgctgac ggcaggacgg
    26581 aatgtatttc ttgccggaat ggactggaaa acactgcccg caagccataa aaacccccgg
    26641 acgtttgccc gcagtctggg ggcagtacgc tatatttcct gtgagtatct cagtacggaa
    26701 gataccgatc gtcacatcat ggttgcagcc gtcagccaga atacactccc caaaggcagc
    26761 cgcaggtatt tctctctcgc catgcttatc ctgcccctgc tcgagccagg aggatacgct
    26821 atcgttgaac tgtcacaggc aaacgataca gaactttatg gttttgtttc tgctgttgac
    26881 ggcatcctgg tcagtgacct ggtgggaacc cgggaggaaa tcagggaggc ccgggaaata
    26941 ttcctcacca ttaacagcgc gcctgaacac ggctggacat gttatgaacc gccctcgttt
    27001 aacgggcctg acggcagagg tcccctgcca ctggaaaccc tgacaggtgc agggaaatat
    27061 ccggcagaag cacgccttca ccccgtatcc cgtgggccac agcttattcc ggtattactc
    27121 atactcaccc ttctgggtac tgcatggtat ggctggcagt actatgaacg cctgaaggca
    27181 gaaaaagcag cacttgctga agcggcacaa aaagccgcag aggaacggat cacaccaccg
    27241 tggctgagta tgccggaaac cggagatttt atccggggat gcagtgacgt atggtcacgc
    27301 ctgccggtgt cggtcgccgg ctggcgatat tcactcgccg agtgtgctgc caccgggaac
    27361 agcggcaatc tgcgtgcatc atatacaaac atcgccggga cgacagtgac cgcattcagc
    27421 cagcgggtca gggaaatttt cagcatccgg ccgtttatgg ttatgccgga cgggaatagc
    27481 ggtggttttg ccctgccggt caccttcagt atgcctgaaa ctcccgttgc cgttgaggca
    27541 ttaccggaaa acaccctcct gcaggaacgg ctgaccacac tcgcccggtc gatgcagctg
    27601 aaaatggact ggcaggaagt cagcaacagc ttcaccgatg aagacggaaa taccatccag
    27661 cccccctgga aggagtatga cctgcagata ctgacgacac ttccggcgca ccaggtggca
    27721 gagcatttca gtgaaccctc cgtccgtttt atctccgtaa caaggcaact ggaagagggt
    27781 cgtttccgtt atcaattcac aggtaaatat tatgtccagt aaaacaaaag ctcttctgct
    27841 ttccgtcctg atcctgccga caacggtcgc ggccagcacc atcagcaaca tgcaacagaa
    27901 taaccggcaa ctccaggtgg aacagattca ggaagaaact gcactgtatg aagccatgct
    27961 ggcaagagaa aaggtgcgac atgaactcag aaccctggag cagggagaac cagctgagcc
    28021 tgtgcattca caggaaagca gttcagccgt cacgacagat acccctgccc ccattaccgg
    28081 tattccccgg ctgacagaga tatccggagt tcacccccag ataaccgcca ggatacaact
    28141 gccgggcgga caaactgctg atgtaaaaga agggcaacac attcccggca ccacattccg
    28201 cgtggaaaag atcaccactg acagtgttgt ccttgaacaa aatgggatca gaacaacact
    28261 acaaccctga ctcagtgccg ccatttacag ccggctgtcg gaacagatta ttcccccggc
    28321 ccttaatcac aaaaaaaatc gtttccgggt accgctgccc gtaaggagat tttatgctgg
    28381 cacctgcaga ccagccacct gatgacattc tggaatacgt ggttgttgat acagataaaa
    28441 caggacaaat tcatgtcaga gtggtggaag gaaaaaaaca ccttcgtgcc gtccaggatt
    28501 atctgacccg cctgtgctcc cgctatccgg gtaaagtatc cccgtttgag ttcaccacac
    28561 ttgatgtgat cgcccgactc aggcaggaga cggtcatttc cggggtggaa aacggaatta
    28621 accccgtaca gcaaaaaatg ctcgggtata tcagccatgc ggccaccctc agcgcctcag
    28681 atcttcacat cacgccgggg cgtgacagta cggatttcac gtatctggag gcccgtgtcc
    28741 acggagagct ggagctgctc gatatcgtca gaaaagatga ggggctggaa ttactggggg
    28801 ccacctattc cggaatgact gacgtcataa agggaacaca gtttgatccc ggtgtccccc
    28861 aggatgcccg tctggctgaa agattcctga aactggccgg actgtttggt gcgcgctact
    28921 cccactatcc gtgtgtgggc ggactctatg ccgtactgcg tctgatcaag gatgacagtc
    28981 agcatatacc gacattcagt atgctggggt atcaccctga gcaggagcgc gctgtccgaa
    29041 ggatgctgca gcgaccggag ggcatcgtga tcctctctgg ccccaccggt tccggaaaat
    29101 ccaccacgct gaggacggct tctgccgcct atctggaaca gtatggcttc aacgataccg
    29161 gagggatcct gctcccccgg cgacgtctgt tcacaataga atcaccacca gaggggcgta
    29221 tccccggtgc aattcagacg gccgttatgg ataccgctca gggctgggtc gattctgtta
    29281 agtccgccct gcgccttgat cctgacgcta tcctgaacgg ggagatacgt gaccatgcct
    29341 ccgccatcac ggcgataaaa gccgccatga ccggtcacct gatgctgacc acccttcatg
    29401 ctaacgaccc gataaatatt cttgagcgtc tggaaatgga gggcgttcag gcaaggatga
    29461 ttgcagatcc gcagttattt atcggcctgc tcagccagcg tctggttcag gtgatatgtc
    29521 cccactgccg cctcccctgg catgaggtgg agagctcccg gacggatgaa gaacgacgcc
    29581 tggtggaaaa cttctgccag cccgatgccg tgtatcttcg taaccataac ggctgcccac
    29641 actgctggcg tggagtgaac ggccggaccg tcattgcaga agtgatttcc ccggatgcga
    29701 agtttttcca gatttaccgg gaaaaaggcc ggattgaggc aaaaacctac tggcaccggg
    29761 agctcggcgg tatgacacga aatcagcacc tgctgggcaa aatcaacagt ggtcaggtcg
    29821 atccgctggc tgcgcattac atatcccctg tagacgagga cagctatacg ctgctgcact
    29881 gatatgaaaa ttctggaaag actcaacaat acggaactgg aactgtccgg ccttaaccgc
    29941 tggctgggca aaaagacgtt caggcggact acgttttacg agtcccttgc aggcatgatc
    30001 cgggacggca caccggttat gcgggcactg gagttcatct gcgatgtgga aacggatttt
    30061 ggtaaaaaga aagggcagtc cggcctgtat tttctggcga cagactgtat cgccagcata
    30121 cgcagcagtg gtcagctctc tccggccctg aaggactggg tacccaaaga tgaaattgcc
    30181 ctgatccgta acggtgaaga acgaggtgat atcgctgaag ccatgtttca ggtcgtcaaa
    30241 acggcaaaag gacgacagga gatgattagc agtctggtga gtgtctgcct ctatccgctc
    30301 atcctgctga cactctgtgt ggtgaatatg tataacgttc atacccgggt tattccgatg
    30361 atcagcgcct ttgcaccgga agaacgctgg tcagtacaga tggtcattct ggccaaatcc
    30421 tccggactgg ttatcggtta tggtttgtgg ctgctggcag gactgctgct gctggcaatt
    30481 ctcatcagga tgtcattcag tctgtatacc ggtccccgac gacggtatct tgatcgcatc
    30541 ccgccatggt ccctgtatca gactttccat ggcgtgaatt acctttttaa tatttcatcc
    30601 atgctgcaga taaatattcc tttatctcat gcccttacca gaatagaaaa acatgcagaa
    30661 cataaccgat ggttaaaatc acgaataagt gctatcagaa aaaatgtctt atccggtcag
    30721 tcactggcac tggccatgaa aaactgtggt tatgactttc cgtcaaaatc ctgtgtgaat
    30781 aatcttcttt taaattcaga aagggaggac agtgttgcca gtatggcgct ttatgcagac
    30841 cgctggctgg aagaagcaaa aaagaatgtc agaaaaagcg gcattattat tactgctata
    30901 tcaggtctgc tggtatttct cttcatcatc aatatggtca gtgccattta cggcatcagt
    30961 gacatgctga agcagtaaac acagcatcct ttatattccc ctcgatccaa attctgtttt
    31021 accaatctca ggagctaata tgagcgtcat aacctcttcg tattcagaac ataatattcg
    31081 tcaccttcag aacgatgata aaggcatgac cctgctggaa gttctcggtg ttctggtcgt
    31141 cgctgccatt gttattggtg ccgtgatggg actgatgtcc gataccctgt cctcttcaga
    31201 caaccagaag gaactcaaaa acctgcagac aatcgccacc aaaatgaaag cccaaaaatt
    31261 ccaggggcag tatacaggta ctgactacgt caaaatcctg accgaaagtg gtggcctgcc
    31321 tgccgatatg atcgccggtg gtaataaggc gaaaaatgcc tggggtggtg cggtaaccat
    31381 caaagtcagt tcagataaat actcctacgt tattgaatcc agcaatgtgc cgaagaaaaa
    31441 ctgtattgat ctggtcacca gcctgcgcag ttcatccatg tttacaaaaa tcaacggcaa
    31501 tgtgacgaat aaagttgacc cgtctacagt atgtaacgca gataaaacta ctatcaaact
    31561 ggaaaccaac agttaatcat tcatcagcgc ccgcccgggc gctttattta aggtcactgt
    31621 atgaacaggt tatttatcct gacagtgatt ttttcactgt tatgctcact tccttccgca
    31681 caggcatccc cacaggacaa acaatatgat cgttgtttta ttaccgccgg aaaacgttat
    31741 tccattgatc caggattact caaaattatt gcccgcaggg aaagcagtct gaatccccgg
    31801 gccattaatc ataacaggaa tgacagagga aaaattatca gtaccgatta cgggataatg
    31861 cagattaact ccacacatat tcccgggctg aaaaaaatgg gcattatccg gagtgagaaa
    31921 gatctgttga ataatgtctg cctgaatatt cagaccggag catggatcct cgcccggcat
    31981 tttcagcgat gcggcgtaaa ctgggaatgc ctcggcagtt ataacgccgg cttcagtaaa
    32041 agtaacactc accgccggat gaaatacgcc cggcagattt acagcgctta catgcaggga
    32101 cgttagtccg ggagaactgc catgctccag ataatcccgg cactgctggt cgccataacc
    32161 gtggtaatac agtgtctggc cgttcagatg agtcgctatc gactctcccg gatgaatgcc
    32221 cgcccccctc accatgggct ggcgctgatg agcgggatgc ttgcagctgc cgccattatc
    32281 ctgcgtcttt atcagaatga cagcgctgtc ggggtgctgt ttatgacgat aatgaccgga
    32341 tgtatgacac tgatgaccct gacggatgct gcatcctgcc gtcttccccg gcccttcacc
    32401 atcatgtatc tgataagcgg cagcgcattt cgtctctggc agggagactg gctgacaggc
    32461 ctgtgcagtg cagggttctg gttcctgctg ttgcttctgt tcaggcaata cacctcttac
    32521 cgcaggaaaa cagaaaccat aggcctgggc gatgtttttc tgattgccgg tattgccatc
    32581 tggtccccgc cgggagacat cccctggatt gtcgcaacgg cagcaggcgg agccttactt
    32641 tatctgtggt gtaacagaca acgacgcatt accagagaac ttccctttgg gccgtttctc
    32701 tgcgcgtcac agtacgcatt tactttttat cccggtggcc tttggtaata ccggagggaa
    32761 cgactatgca aaaagacaac gataaaggtt ttgcacttct tgaaattctg ggaggactgg
    32821 ttgtcatcag cctgctcatg ccgctgttct ggtcttacat tgaagactac ctgaatgaaa
    32881 tgcgcaacca gagcgccgca tttcatgcag atgcctacaa tactgcagcc aggacctaca
    32941 tcgccgataa caatgcccgc ctgcatagcg gaacgcttcc tgccaccttc acggcggatg
    33001 agttaatccg gaaaggatat ctgaagggtc ttaaccgctc gccctttggg cagagttata
    33061 ccaccggcat ccgtcgtaac acgagtaccg gtcgtctgga ggccctgacc tgctccaccg
    33121 gaggggagaa tatcaaagac gatgccctgc gcagtatcgc cagcctgctc cccggtctgg
    33181 gcggatttat aggaaaaaat ggcacagcaa ccggtgtttt tggtggatgg acagataaac
    33241 ccggcgacta tggactcagc tgcaatggcg ggcacatcgc catcgtaatg atgggtgatg
    33301 acctgcagga aagcgatcgc ctgtatcgct ttcaggtacc gggacgaccg gagctgaacc
    33361 agatgaacac ggccatcaat atgggaggga acaacctgaa caatgcagga aacgtcaatg
    33421 gacaaagtgc aacactgaaa ggtgatgtta caagcgaaaa cggctggtta atcaccaaaa
    33481 atgataaagg ctggaagaac atcacctatg gtggcggttt tacgatgaca gacagccagt
    33541 ggatacgcgc agtcggtggt aaaggaatta taaccacagg ggaaatcaaa ggaggaaaag
    33601 tcagtggggg gacagtccgg tctgacggtc gcctttccac aggtgagtat ttacagctgg
    33661 ataaaacagc cgtagcaaat acaaagtgtt caccagatgg acttgtaggc cgcgacagca
    33721 aaggggcaat actttcgtgc caatccggtg tgtgggttgg ctttgaatcc taccctgtgg
    33781 gaagccctat cccgtggcca tctgccactc ctccacaagg ctatctgttg atgaacggcc
    33841 agtcgttttc ctgttcccgg tatcctcagc ttgcccgtgc ttatcctagc tgtaagttac
    33901 ccgatctgcg cggtgttttt atccgtggct gggataacgg gaagggactt gatggtgatc
    33961 ggaacagaca gctcctgagt tatcaggccg atcaatcagg cgtctatcac gaacgtggcg
    34021 gatggctgaa agggcatcat agtggtatgc catactgggc acaggggagt accacagaaa
    34081 tgcgtcccaa aaacattgca ttcaactaca ttgttaaagc cagctaggga ctcacatctg
    34141 ataggtatac acagagaaaa caccgttacc acaggcgtaa ttttgtgttg gtctggagat
    34201 gatttgatat gacgctccca ctggcacggc aaatgaaata aatctccggg catatcagct
    34261 ggtcagcaaa ctcgatgcca ggcgtaaaca gaccagcaaa atttcccctt attttgttcc
    34321 ctaccctgca actgtcgggt gcagaaacgg gctgtttgtc gtgacactgc acgatttcga
    34381 ccttgaaacc cgtgcagaaa cccggggact ggcaatatct catgccagca tgctcagtca
    34441 cctcctgtcc gaatcctttt cattcgccct taaaacagag ttgcaaacac tcttgaaaca
    34501 cactcgatca taacgatcga aataactcgc ttccgtttaa aaaacactct ctttagactc
    34561 aatttatgaa caaaacacgc tgacccgcat ggaggtgaaa tgaaaactga cagcacaggt
    34621 attgccgccc gtatgatgct ctccctggac agggagcgaa tttgcgaatg cctgctcagt
    34681 catcgccagc tgcagtccac acccctgcag gttcgttatc cgcagggagt cagggatgcc
    34741 cttggtatca tgagcgaaca gctttctctt tccgtctctg acctgacgcg tatcctggtc
    34801 gaagacgcgt taagtgaaat gtttcttcct gcagataaca tcgtccgccg tcttctcagc
    34861 aggatggagc acatcatgca ggcacatgat atcagcgcaa caacgatggc tgcattactg
    34921 gccccctgga atatccgccc tgccgtcttc cgggagccgg acagactcac tgactatctg
    34981 accggggaga ttcttgcagc actggctgac tggttttatc tcagtccgga atggctgaac
    35041 ggcagggtgc attatccact gtatcgtccg ggagactggc cagccacaca ggagatattc
    35101 tgcaggatta tttcagcccg cgagaatatg gacattattc tctggcatgg ttttccgttt
    35161 gccggaacgc attccggaga atattgcggc gttctgctca ggcagaaaaa agaaattaat
    35221 aacaccatta tttatccggt actttcgtta tatccggcca gaatggatat agaaaaagaa
    35281 ggctggtttc agatggcccg aaaaatatct ccggatatcc ctgttcgcgc cgtaacactc
    35341 actccggcac aggcagaata tctgataaca ggaaaaatat tacccactgc gttattcagg
    35401 gttccgttat ccccctggta ataatactga gcaataaacc ggaataatac accaactgtt
    35461 tttaatggca cagccgtgcc ctctgttgtt atacccaaaa tacctcaata caggaaactg
    35521 aaatgatgat gaaaaagcat attacccgta ctctcatcgc gtctgccgtt ttattttcct
    35581 ttaattcgac tgcagcaacg tcatattttg aagcccgcaa tgatgcgatg ggaggaacgg
    35641 gggttgcctc ctctcactat ggtgtggccc cactggcaaa cccggcactg ctgacaaaat
    35701 ccggtgcaaa agatgacttc agtcttctgc ttccgtccgt gggtgcgcag ctgtccgatc
    35761 cgggtaatat cgccgacaac gccgacaaaa tcagcgatga ctggaaagcc ttcgaccgtg
    35821 ccgtcgacag ccactccggg gtaccgcaga cagcggcccg cctgaaagag agactgcagg
    35881 acttccggaa cacccatgcc agcgcacagg ccggggtatc tgctgtggcg gcccttcccg
    35941 gtgataccct cgctgctgca ctgatgctga aaacgctcgg gactgtcagc gtcgacggta
    36001 aagtcagcga tgccgacctg aactatctgg aaagcattgc agacagtggc agccaggatg
    36061 tggataaaaa cagactgacc tcacaggctt ttgcccgtgc cgcactgatc accgatgtgg
    36121 gcgttgcgct ggccactgaa ctggaaacgg ccggacaaaa atggtcactg ggcttcacac
    36181 caaaattcca gcgtgttgac ctgtttaact acaatacgct gatcaaaaac tatgacagca
    36241 gcgcattcaa aggtgaccgt taccacaaca cccaaaacgg tattaacgct gatatcggtg
    36301 ccagcatgga cctggatgac aactggacgc tgggtctggt cgcacagaac ctgatcccac
    36361 gcagcattga gaccaaagag gtacatggtg tcactgaaac cttcaggatc cgtccgcaag
    36421 taacaaccgg cgtatcatgg cacaacgaca tgttcaccac ggcactggat gtggatctga
    36481 ccccggccag cggcttcaca tcagacagca aacgtcagtt tgccgccgta ggtgctgagt
    36541 ttaatgcctg gaaatgggca cagttacgta ccggttaccg ccagaatctg gccagcaaca
    36601 acggcagtgc attcactgcc gggattgggc tctcaccgtt tgatgtgatt cacattgatg
    36661 tcgccggcct cgtgggcacc gacaacaact acggtgccat tgctcagtta cagttcacct
    36721 tctgattatc acgccccggg acgcccgggg cttctgacct gaatctgctc gtttccgctc
    36781 ttctggccac tcaccgccag tcctgttcat tattttccgg ataaaaccat gcaaaaaaaa
    36841 cgtatcaccc ttgcaggggg agttgccatg ctctgtctga gcggctgtca tcttcccccg
    36901 gataataccg caccggccag gaagtacggt gcagcttctg aagtcgctgt cacacgacat
    36961 gcacagtatc agttgtggga gcagggcacg tattctgcag ccctgccttc ccgccagccc
    37021 ggaggcagcc tgcaggctca cagcgggcgg gtttcctttg actgggaagg cgatgctctg
    37081 gagctcctga gcgagctggc ccgtctgcgc ggacaacagt tcagttacag cggcgtgcgt
    37141 cttcccctgc ccgtctcact ccatgtacgc gatatgacct tcgctgatgc ccttcgtctc
    37201 acagaggccc agactgcctg gcgggcagtc ctctcacagt cagccgggcg tttacatctt
    37261 tcctatatgc caacggagaa aaaatgaaaa aacaggtctg tctcctgctg tccgggctcc
    37321 tgctggcagg ctgtgccgga cagcaccatc gcccccctgc cggtatggca ggcaatttac
    37381 caccgccgga tctccgggca tggctgacgc ccactgacaa ccaggatgac ggcatttcag
    37441 acacccgtcg ccagatgctc acggacgccg gacgaacgct gggattccgt ggcggaaaag
    37501 cacagcgctc atgggaactg attcaggcac tgaatgcccg ggaaagtaca ctgaatgccc
    37561 tgtatgactt ccgtcccctt atcagcaggg aaggctggct accccctgtt attgatgaag
    37621 cgcaggatgt tgctcacatt cagcctgacc agataagaac cgcgtcccgt gtctggacca
    37681 tcctgcgccc cgaacgtttt gtcagtaacc cgccgggctg gcgtgactgg cttctgcggg
    37741 ggctttccac taccgccacc ccgggaactg agggccgtgt tgtaccggaa gaccgcgcac
    37801 agcgcagact ctgggaaaac gcactccgcc agggctggca ggaaggccgt gacaacgctg
    37861 acctcaccct ggaagccaac cagaaacgcc tgacccggga ttaccgggga atgatgcttt
    37921 atgccctgct ctggcgtcag ggcatgatca cacgcccgga tgtgactgaa cagcggcaga
    37981 ccgtcaccgg taacggcagg aaactcatca ccggagacca cgtccgccgt ctgaagacac
    38041 acgcggaatt caccctgcag aaatcacgct ggcgtccggt cgtcagcacg gaaggagccc
    38101 ccgatgaaat tacccgatga gttcggcaca ttccctttcg cccgtttcac ggccgaagag
    38161 tttcgctatt tttttgcctg gtgtgcccga caccgcgtca gtgatatcga tctgacaggc
    38221 ggctcaccgg tatctgtcag tcgctttgga cggcgggtac gttcgtccgc acatcctctg
    38281 ccgtcctcca tcatgatggc gctgagtgat gaattgtttg gacgggaagt ccttccccgg
    38341 gtgctgtccg gtcatcccgt ggaccgtacc attcaggtca gcggtgatgc ctccggtcgc
    38401 tacggtctgc aacgggggca gcgtgttcgc ctgcgctgtc atctgattca gggaacatca
    38461 gcaacagaag aaaaagtaat ctccgtcacc atgcgggtga tcccgtccga tattccggac
    38521 atcctcacca tgaatattga accggacctg ctggatgcca tgctccggaa aaccggtctg
    38581 ggtttcatct gcggagagac cggctccggt aaatccacac tggcagcggc actgtaccgc
    38641 tacattcaga cgcatttccc cgaccgcaaa acggtgacct atgaagatcc ggtggaatac
    38701 attctggggg gagagaatga ccttctgcca ccccatcagg ccgaaatcgg gcgtgatgtg
    38761 gaaagttttg cggcaggact tcgctctgcc gttcgccgta acccggacat catcggtatc
    38821 ggtgaaatca gggattatga aacggcagat gcagccgtcc aggcaggtaa caccggtcac
    38881 ttctgcatcg gcaccatgca caccaaatca cccggtgaaa cctttgcccg gctgctgggg
    38941 ttatttccac cggaaatcag ggattcaatg gcagccgcca ccctttccct ggtgcagttc
    39001 attctggtgc aggtcctggt cagaacaaat gacggtggca gacaggccgt ccgggaatac
    39061 atcgtcataa ctgacgaact gcgtgatacg ctctcccggc agtcccatgc gacatggggg
    39121 cattacattg atgaaatcat tcgcaaggaa aaacggcgga tacgggatca ggttctgacc
    39181 atgtatcaga cgggcagcat tgaggcatca gaagctgcgc tcttcatccc ggccggagaa
    39241 ttccccaaat gaaacgcgaa ggcttctggc gggatgtcac tccgccactg aatatttatg
    39301 gtgtcccgtt gccgtttacc atgatttacc tgctgctgtt tccgtatccg tcaaaaacaa
    39361 cattctgggt gtgcagcggc attttgctgt tttttgtgct cctggggtac tcaggatgga
    39421 ctgtccggac gctctgccgg cgtctgtaca gcctggtgca gggtaaacag gcgtcaggcc
    39481 gtccctggtg gtaccggcat cgcacagaat taccccgtga atggcgcgga acggaataac
    39541 cctcttttgt tcatcatccc ggcgtatcac aacgccctca tctttaatcc ccggagaacg
    39601 acatgaagtc cagatatcga acctggcttg ctgtcccgcc tgaggagaca gaagccgtaa
    39661 aaaatgcagt tcccccgtta aatggccgca aagctgtcgc atgggatcca gaaaaaaagc
    39721 tctggtatgc ccgggcgggt accgaacttt cactgctgga gcgctggtta ccccggccac
    39781 aggaactgtc gatggatgcc ggcgatccgg tcactgaatt tgctcaggtc ctggaaaacg
    39841 ccgggctggt tattcaggga ttgccacaga tggacggagc catacacaga gtggccaccc
    39901 gggatgacaa aaaaggggcc aaaagtggcg catacagggc ctatcttgat ggtcgtcccg
    39961 ccggctggta ccgcgactac cggagcgccg atgacagtcc cacaaactgg gtattttccg
    40021 gcggagaaca acacgatcct ctggccagac tgcatctgcg ggcattcgct cagcaacagc
    40081 gcgatgataa cgctcgtaaa ctgcagcaac agtataacaa acaggccggc tatgcccgct
    40141 cctatattaa caggttgcca caggcaacag cacatgaata cctgacgcgc aagggcatcc
    40201 gggccgcgcc tggcgtgcgg ctgaacaata aaaatgagct ggttattccc ttcagcaacg
    40261 gccggggaga aatacgttcg taccagcgta ttccggtcac cggcgggaag gatgcccgca
    40321 ttctgaaaga ctccgagaaa accggcaact ggttcacctt cggaaccccg gaaaatggcc
    40381 gccccctgtt atttgcggaa ggttacgcta ctgccgcctc cctgcacgaa gcaaccggcc
    40441 ttccggttct gatgacagtt gatgccagca atatgattgc cgtggcagaa aatgcccgcc
    40501 aaatctggac tgacagcccc tttgtcttct gtgccgacaa cgatcatcaa cgggaaatta
    40561 acaaaggggt ttttagtgca acaaaggccg cagaagtcac gaatggcgaa gtcattattc
    40621 cggcctttac ggaggcggaa aaagcacagg gactgaccga tttcaatgac ctggacgcca
    40681 gccggggacg ggacaacttt cagaatgcca tgaatgcaca gttaaaacat atcggtatcc
    40741 ccacacccaa cagtgacact gccgaccatc gtgaggctgt cgtgatcgac aacctgatat
    40801 tcacaccggt aaaagatgag aagccacaaa tgtcccctga aaacagacag agcacagccc
    40861 cggaaacgga gctggcaaca caggatacgc cttatgacac ccactccgac gatacagaaa
    40921 accaggcata tgaagatgac tatgccgcct atgcatccct gcaatcagta actgcggaga
    40981 cgttatcccc ggataaacca ctggagaata ttgcgtctgc cgctgatacc ggcacagaga
    41041 atacctctgt gcctaccgct ggcgaccagg aagacctcaa tacagacgcc acaggtacag
    41101 acaccgctca caacagcgac acagcagaaa caggaacgct tcctgaacct gccgggatgc
    41161 agtccgtgca gcaggcaacc actgaaccga ccgcagaaga taaaccacac gagaacgttg
    41221 tttccgctgc cgataccaga gaagaaaatg cctctgtggc taccgctgat accggtgaag
    41281 aaaatgcctc tgccaccacc gctgacaacc agagcacgca ggcagaagaa catccccgga
    41341 gtaaagacac accggaaacc tccctctccg acgcggccgg gacccagcac accaccacaa
    41401 cgcagtctga agaacaagtg caggtttcca tggccagcgc tgacgactgg agcgcatttg
    41461 cggaagatct caacgctgac actccggggg cccccactgt tcacaacacc gacaacgcgg
    41521 agacaaacac agtttctgaa ccggccggga tgcggccagt tcagcaggtg accactgaac
    41581 tgacggcaga agataaagca caggaaaaga gcgttgcgtc tgccgctgag accggcgcag
    41641 agcactcctc tgacatcacc gatgacaaca ggaacgcacc cgctgaggag ctcaatgccg
    41701 atgccacggt tgctgttcac aacaccggca caacagaaac agcctcacct tctgaacctg
    41761 cagaaactca gccagtccgg caggccaccg ctgaaccgac ggctgaagat aaactgcagg
    41821 aagagcgtct tgtttccgcc actgagacca gcacagcgaa tgaagcagcc cgccctgaaa
    41881 cgccatcgct tttcagccgt cttcgccgtt ttctgaccgg tgtgaaatca cgcacctcag
    41941 aaccttccgt tattcaggag aaaaagccca cggaggctga aacagcaaca gaggaggtcg
    42001 caccaccatc accaccggaa accagcccgg aagacacatc gtcagcggaa ccggacacga
    42061 ttatctttgc accccgccgt cccgacagtc cgcagcggca caacctggac gagatcctta
    42121 aaaatcttac acacaaagag tttcccgaca ggactgcgct ctacagcgtt gacggtgaac
    42181 ccgccttcct ggacagactc tactgtctcg aaatggtgga tggcgccagc gcggatgaca
    42241 aaaaggtcct ggctgccctt gctgtggcca ccaacttcta tggcggcgtt atcgagctga
    42301 ccggcagtga tgcctttaag caaaaagcca tgcagctgat tgtcgaatac gacatcaagg
    42361 ttcgcatgaa actcccggcc cagcgggcag cactggaaaa aatccgcaag gaaaaagaga
    42421 tggcgcatga tgccattgtc acccacagac ccacgccgga actcaaccgc cagtcaccgg
    42481 aagagacaac aatggcccaa ccggctcccc ctgtgtcacc gaaggcagag gctgaacaaa
    42541 cagcaggcag tacggctgaa acaccgcatc cggaagcgcc ggtcacggca aaagctgaag
    42601 ccccccgggc cgacagaccg gaacaacccg ccgccccgtc gtctgcgcca gaaaaagccc
    42661 ctgtcagtgc acagccgcag caacccgaca caccggagga agagccgccc ggcaagctca
    42721 gccccggtga aagtgtgact gccgtcctga caaactacgg agaaaaagag tatgcgcccg
    42781 gaaaagggcg ctgctttttt gtggaactca ccaaccgcag tggcaaacgg gaatactggg
    42841 gaaacggcct ggaagaactg gtcaaaaacc atcagaaggg tgaccccgtc acactgacac
    42901 tgcaggcgag agaaacattc cggctgtccg gtgaaacgcg ggatcgtatc cgtaatatct
    42961 gggccataac cccggtcagt aacggcatta ccgttaataa tgaccaacct catgcgggtc
    43021 agcataccct ggagtttccg atggcaacat tcagccaggt gatgttacag atgcaacaac
    43081 actggccaca gataatgcag gaagttcgct cccccaaaaa tcctcccaat aatctgtaca
    43141 ttggtgaaga caggcaacct gcatcagcac ctcaggatca atcaatgcga ctccccctcg
    43201 ctggtaatgc ccccaatgaa ctgataccag ccatcgcctc tgccgataag gacaatcgtc
    43261 agttgaatct tctgctggtc cacagcgcag atgatcatct gcagggcgtg gtccgcctga
    43321 acggcaccct ttacccggca ctggccaccc cgtcagctga caaccgtcag ctggttatca
    43381 atgccctgac ggataacggt ctgcagtttg ccggttacgg tgaagctgtt aaccatgatg
    43441 agaataatca tcagcgtccg tccccacaaa taatgcagtt tcacctgaag caacaggact
    43501 ccccgttgtt tgccgccatc cacaaaccgg aagagcaacc ggacaagctt ttccgctctc
    43561 tgggttttga gcagacctgg aaagagtggt ctgacagcca aaaggcggag gacaggcagg
    43621 aaaaaactct gcaacaggca caatcccaca gccccggccg atgagccggt aaggacgacc
    43681 atgaacatga aaaaaacgct gctggtcata ctgaccggca tcaccctggc atcaccggtg
    43741 gcaggtgccg ccacctgccg ggcgggggcc gctgccgaaa ccggcagcag agccgcctgg
    43801 gagcgggcgc gcacagccag ccaggcatgg tcacagcggg agaatgacgt cgcctcctcc
    43861 ctgcagtcct gcctgtcccg catcaaggac atcagtatca ctcttccgca gttccccagc
    43921 ctggacaagc tcctccgcga cctggaaaac caggtctgtg atgccgtcgt ggatcgggtg
    43981 aatgaccatc ttccggggaa tatcgacccc tggaaagact acaacctctg aacaggagca
    44041 ccatgagcga agcacaaccc acctcccctg tctcaccgga agcggataac agcggagata
    44101 tttataaccc cgtgctcaga accatcgcac agtcagaaca acgggccgcg ctggtacccg
    44161 gtctggtcag ggcgttgctg tacagcggcg cagcactctg catcagcctg gtggctaatg
    44221 gcatacagta ctggcatgcc accggcgtga cgcgcgaata ctttgccact gacaatggcc
    44281 gactggtccg tctggcacca accagccagc cggcctggag tcagagcgac gtcatgaact
    44341 ttggcagcat gacgctgagc gaagccttca atctcgattt tgtccactac cgaaaacaaa
    44401 tcacgacaca ggccccccgc ttttccgaag ccggctttgc cggctatgtg aatgcccttc
    44461 aggcctcgaa cattctcgac actattaaaa aggagaaatt taacctcaca accactatcg
    44521 gggcaggtac cctggtcaac cagggacaga tggagaacgg cgtctggttc tggaaattcc
    44581 agtatccggt caggatgcgc ctggtcggtc agaccagcac ccgccccgaa caggccttca
    44641 cctttgaaat cactgtccgg cgcgttgacc cccgcatcaa acccggcggc atggaaatca
    44701 gccagatggt ttcccgtaac gccgcagcag gaatctgaga ctcatatcat gcacatacat
    44761 catcttattg gcgcactgct tattgtgtgc ggcactgtca cagctgcaga taacccgggc
    44821 tggaaaaatg cacgaagccc gcagaccggc ggaaagaccg cacaggcaac cgggaacacc
    44881 ccggcaggcg gtcccccgcc agccgcacaa ctcccgggaa acctgccgcc tcccggcgct
    44941 gctcttgtac aggaaggcgc atcgctggat tctcccctga atgccagtga aattcgtgaa
    45001 ctccgtggga taatggccga taacgaacgg gccatcagtg ccccgctcac cagtgtggtg
    45061 ccccgtatca gttccctcac ggtgaatctg tctccggggg ccagcctgcc ccttgtccgt
    45121 accgctctgc ataacgtcag cgtggtgacc ttcacggacg tcaacggcag tccgtggcca
    45181 cagtcggatc cgccctataa cgctgcgccc ggtcactttg acgtgcagtt caacggcaat
    45241 atggtgtccg tcacccccct gcgcgcctgg gctgccggga atgtgacggt ctatctcaaa
    45301 ggattgtctg taccggtcat cctgaatatc accagtggtg aaacggacac cactaccctg
    45361 agtcaggaaa tcgacagtcg tctggatctg cgcattcccc ggcggggacc tgccagcccg
    45421 gtggtgtcca taccgtcaga aaaaattgcc ctgcatgacg ccactctgca ggcctttctg
    45481 gacggtgtgc caccggatga tcccgccatc cgccgcctga aattctccgg taacgtcccg
    45541 gacaccacca tctggcaaca cggcgatgat cttctcgtgc gctcacgggc catgctgcgg
    45601 gatgaatttg aacagacgct ggcatcggct gatggtacgc ggctgtggaa actgccggtg
    45661 acccccttac tcaccttctc cgtgaatggt cagtccgttc acgtcacccc ggaactggag
    45721 taaatcatga gcgcagaaca agaacaggac acagccgcaa aatccggcaa aaagctgtca
    45781 gccctgctgg ttatgggggg cgtgattatc atcgggggcg gatacctgct gctcacctgg
    45841 ctgtcccccg aaaacgctgc atcgctctcg tcagtcaata tcaacagcac aacgtccggc
    45901 ggcggacgga acgtgatgga gacacagcac taccgggatt tactgcgggc tgacaatgaa
    45961 cgtggtgctg cagaagcgtt acgtaataac gagacgttta tcgccagtct gccccgggga
    46021 ctggataccg ataccggaaa cgatcagaaa agggataatc cccccgatga tcagtcaggc
    46081 acttccgggc aaaacgcatc cccccctcag gaagatcaga ctgcccggga aaaacggcgt
    46141 gaggcattac agaaactgct tgcccgtatc aatacacagc atcaggatgc ccggccaccg
    46201 gtagtggcag cagccatgtg gagcaccacc ggcactcccg ggacagcccc acatgcgtca
    46261 ccgtccggta ccgttacatc acagcatccc cgtctgcagg agacgagtct ggcaacaccg
    46321 gctacacgtc aggggatcca gctgataccg gcactgaccc gcgtaccggc ttacatcgac
    46381 accgaagtgg actctgacaa tgcggtctcc cgtgtggtgg cctccgtacc tgccggccca
    46441 tgggccgggg ccgtcctcta cagccaggac gccagactcg caggtcaggg aatggatatc
    46501 cactttgacc ggatgatgtg gcggggcatg tttctgaagg tgaatgcata tgccctgaat
    46561 gaacgaacag gaatgtcaac cgtggccagc gacgtgaacc accgctggtt taagcacatc
    46621 gttctgccgt ccgtacttgg cggggtgggc aatgtcggaa gtctttacaa agacgccaac
    46681 acacagatcc ttcagggaaa ctacggcagt attaccggca gggtgggaat gcctgatgga
    46741 gaggcgctgg ccggcgtcat tgcaggtggc atggccgaac ggggcagcca gctgctgacc
    46801 cgccaggcag agagcgaacc ttaccgtcag gtgacagtat cacggggcga ggtcgtctcc
    46861 attctgtttg ttgagccggt catgagcaac gatatcagta cgccgtcctc cggcactgcc
    46921 ccttctgagt ccccctcctc cggacgtccg ctcacccagg cggaggcaga gcaccaggcc
    46981 tctgacagga tccgggcggc ggtcctccgt aaacagaatg aaatgcagca acgctacggc
    47041 atacaacagg aaagttatcc atgaaaaacg aacatgaaga tcccggcagc tttggcgaaa
    47101 cgacggcacc ggcatccggc aaatccttcc tgaaacgcca gtatatggga tacagcctgc
    47161 cggtctgggc cggctgtgcc ctcatcatcc tggtcgccat ctggtacaag tttctgcgga
    47221 caccctcaga gccccctgca gatgcaggct ttgctgtcac accgcccgct gctgagagct
    47281 gggcagagcc catgaccatg acggagtccg ggatcggaaa caacatgccc gcaccgccgg
    47341 ttaccccaca ggcagacatg cagcaacttg cccgtgatct gaaagcagag ctggacagac
    47401 gggatgagga aatccggcgc accctgacca tgctgaagga cagtatcacc accctcagta
    47461 acgccattca gaaggatgag gcctgggcac aggaaacccg tcagttgctt gaggagaccc
    47521 gtaagcaggt gcataccctg cagatgcagg gagcactgcc agccccgacc gggaataaca
    47581 gacagcctgc gaaaaccctg ccttcacctc tgcagggcat gaaaatcatg tcggtggaga
    47641 aaggcatggc ctggatccgc tggcagggca gtgcctgggc agtacgggaa ggcgacagtc
    47701 tgggaaacat caccatcacc cggattgacc cgtccagccg ggcagtcatc acctctgccg
    47761 gtacattacg atgatgagca ttgatgccct gacagccata gagaactttg ccagcggtat
    47821 tttcagtgca ggcatggagt ttctctttac ctggggagag ctgctgggga ttatcggcct
    47881 tatcggtcat ctgatgcgcg cccgggcaga aggacgtcac agtatgggac cgggaaaatt
    47941 tattgccggt atcttcatct gcggcatgct ggtcagtctg ccctcaatga tcaatgccgg
    48001 tggtacccag atgggcttca gggcagattc tttcgccccc atcgcatacg tgcagccggg
    48061 ctcctttggt gccgctgccg gtgccgtcaa tgccattctc tcactggcaa aactggccgg
    48121 cgtcggtttt gtcatgaacg gcatatccat ctggcgcaaa tcactgctgg acgggcacac
    48181 cgccctgagc gccagtgaat ccatcagtaa agggaacgtc aagtttgtgg ccggcgttct
    48241 gctggttttc aacgaccggg ttatcgatgc cactctggca agcctcggta tcgccttctg
    48301 acccctgtat tttttcagta aatcaatctg ttaataaagg agacactatg tcacgacttc
    48361 gtgccatggc ggcgcgtctg cgcgctgcat ctgtttatgt tcagttatgc ctgatggcct
    48421 gccgtgaccg gctggtacgc ctgttactgc tgcttcccgt gatgctgctg ccccacaccg
    48481 caatggcaaa taccctcttc gatatgatta aaaccgttgg cgatggtgcc agaggctcca
    48541 gcagttcgtt tgtggctatc ggtcaggtta ttggcctggt gctctttatc ggcggcctgc
    48601 ttggcctcaa acaaatcggt aaaaaacccg gtgcagggct ggcctgcctg ctgtcaattc
    48661 tggtcggcgt ctgccttgcc gtcgtaccgg aactgatggg cagcggacag aaaacgctcg
    48721 gtctttctcc ggtttcaatc agttaatccg caaaggcacc gggaaaccgg tgccgtcttc
    48781 acacaatgag gaggaccaga ccatgtcctg tgagctaccc cctttcaccc tgctcagaac
    48841 agagcacggc aacgtgattg cccgcgacaa ccgggataat caggagtttg tgtttgcaga
    48901 gtgttactcc accgctatag cagcactgat tctggctgcc gtcaccctgt atacagagag
    48961 aaaagcgtat gaaaaagacc ccgccactca tcgccccggt ccgggaacga tggcttttcc
    49021 cgctcccgca accggacacg gtattcagcg gtgaagccct gttaccaccg ccagtgctct
    49081 caacagaggg acgctgcccc tgctgtcgca ggaccgtcac ccaccgcttc attctggaag
    49141 attcatggcc attacagcag atggctgaca cctgccggga tacggtcgtc ctgctggaaa
    49201 aaaacctcac ccgggtgatg cgcctgaaaa aacgtccggt accggaaaac gctgacgaaa
    49261 aaaagaaaca cacccgaacc ctgcaggatg cggagcggtc cctggcacag gcccgcctgt
    49321 cggccaggcg actggccctg cgtcatgtgg aaaagtcgca aatcgtcaca acggatgcac
    49381 tcagcgaaaa tgaaagtgac ctgctgcagc cggaaggccc gccctttcat ctctgtgcct
    49441 tctgccatgc ctggcactgt ctgaacggct atgctgccgc acagggagtg atggtctggc
    49501 tccccgattt gcatccggcc tcggtggttg ccctcaatgc gcgggcactg caggagattt
    49561 tcagcgataa ccgtcagcgg gtcaggcagg gacgggcagt cctgaatgcc ctggtacaga
    49621 accgtctggc cgttgaggag aaattccgca cctggcgtcc ggcggatttt gctgatgccc
    49681 tgcgccgctg gccgccggca cagcgtaaaa cgttgaggga gaaaatggac ggggtggcgc
    49741 tcattctgct gccggattcg tttcctgaca aaaagtacgt catgtgacac acaccggtgc
    49801 cggtgacgtt ctgccggccc caactgacag aggaacaaaa aatggatacc ctcactgccg
    49861 gatatcagtt ttccgcagat gcattccggc gctttattat ccggacgctg actgacgata
    49921 ttccgcctga cggagatgaa aaagagattg cccggttcat tctccgccac ccggcattta
    49981 acggcggctg gctgctcttt ctggctgcag aaattcatga aagatcccgt acccataaca
    50041 gcatcaccgt cacagtgagc gtcgataact atctctcatg gatggatatt gccgtgtccg
    50101 gtctcccgga attaccggat atgcccttct gtgatttttt cattcgccgc cagcagttta
    50161 tttatccggg tgtacccccg acaaaatcag gagaagaatg atgaaaattc accgcatatc
    50221 cccggagacg cttataacac tcatccacgc acacctggcc ggaaaagctg acagtaccgc
    50281 caaagaagag caccggctac tccgtcgttt cctgcgggat gatgacggca ggctggccgg
    50341 cgtactgctg aatattgccg gaatacttca gttcaacagg gaattaagtg cgcggcataa
    50401 ttatccggcc acacccctga ctgaattttc attaaggaaa cggggaaaac aacttcatct
    50461 ctgtctctgc agtctccgtt tcttttatat tcctccggta tttatacaaa acaaaagaag
    50521 aaaatctatt gttgtacacc ttaataagat cacttataaa caaacccatt caatacgctg
    50581 acgaagtgat tcatactgat tatactcatc tttgcctata tgttttttaa gcgtattaac
    50641 aatatcatat tgaggatgtc ctgaattgtc agtatacagt gttgttgcag gaaggactgc
    50701 ataatctgat atttcacaac ctgtatacag ttctttgtca gttcggcagg aatggcaaag
    50761 agaaagacat gtatacaggt ctgtgttttc tgcctcctca ggagataacc acgacacata
    50821 ttgcaaatca tctgtatcag gataacaatt ttcttcctga acctgaactc tgaaaatata
    50881 gaaaaataat tcatcttttg aaaggtatgc tattttattc ccccaatgca ccgcattatc
    50941 aagttgatcc gataaataaa tccatcctgg tgtcgtctct aacgaccagc atataacaga
    51001 aggagcatca gttcttattt tcccatcttt tattatttgc aggcctctgc tcattgttgt
    51061 cgcatgaaaa agatttaata ttttattttc agccttcact ctatttcctc atttcccaag
    51121 aaacatatta aaaacggaaa acattacaca taacctggag gacaaatcat gactttaaat
    51181 ttccctgccg gggaaactac actattctca gcaaaaaaca tcctgacagc aatactcact
    51241 cagcacaact tacacgcata gcgttcccat ttgaatacag ttgcagcccg cgatttattt
    51301 tgtcttccgg agccacctct gccatgaata tcaaccgcat tatttttgcc attgaagact
    51361 gtatcagtac cctgtcacgg tattccgttt ccgcattctt ccccgattac tgtgatctgc
    51421 acaccgtcat cggtctggac cagcaggacc gcgaacgccg cccctggctg aaagccccct
    51481 acattgccac cacaaaacag gggtattacc tttccgtctt tgaaatcagc ggtgccctga
    51541 aagaaatgga tgaaaacccg gaccagacgg cccccgggac actggagtca ctgattgcag
    51601 cactggctga gcgaatgaac acggcctgga aaaattccgg ccacaaaatc agttttgttt
    51661 tcgaacgcga tccggagcgt ggaagggatg aaatcgaagc catgctctta ccccagcgaa
    51721 aatccatcgc acgcacgggg atcctgcttc aggacgtcct ggatgaaaaa gtcaccacac
    51781 tcaccccctg gcttgtccgg gagcgctgct ggctgaccgt ctggagctct gaagaactgc
    51841 tgagccggac cgatcgcaag gaccatcaga cccgtgtcag gaaactagct gaacacgctc
    51901 cgccagcccg ttttgcgcag gatccctggc gctggacact ttccgccctc aaaatacggc
    51961 atgacgcctt actggacacg ctggagcagg ccctgacaca tgattctgat ggcctgctga
    52021 ttcgcctgat ggatatccat gaagtgggac gggaaatccg ccgccagctg gagcgcaaca
    52081 gtacaccggc agtatggcag cctcatctgc ctgaagatgc ccgtcctgcc ggctggcgac
    52141 agggcgggga tacctctgta ttccacgcac cgtcgctgaa tctgcagtta ttcaccacac
    52201 agcctgaaac acacggcagc ctgattcagg ccggtgagct ctggcacggt atggtcgcca
    52261 ttaccctgcc ccctcagaat ctgcggacat tcaaccagct ggtcagggat gtgccccgtg
    52321 caatcccctg gcgtatccgg atggatctga tgcccgacgg catgaaggcg ctgggggtca
    52381 agaaaaccct gctgacctac agcagcttta ttcctcctct ccggcccatg tatgactcgg
    52441 taatgatgct gaatgacatc aacaagcatg atccggtctg tatcatgacc attgttgcca
    52501 ccacatgggg aaacagccgg gaagtctgta cccgtaacca ggctctcctg aaatctgcgc
    52561 tggaaggctg gggggtatgc ggcaccacca caaccttcgg tgatccgaga cgtgcctggg
    52621 tgaacacggt tctggcagcc tcccacagct ccggcccgat ccccctttac ccgccccttt
    52681 cacacgccct ttccctgttt cccctgaaca gggcaggctc tgtctggcgg ggacagggaa
    52741 atctgatgat gcacaccgaa gacggctctg catgggaggt ggcgctggcg tcatcacaac
    52801 agaacaagca cacggagctg acacccggcg cacccggtct gggcaaatcg gtactcatca
    52861 acgcactgtc ggagatccag atagcctcag cccagaagaa tctccctttc attgcctata
    52921 tcgacaaggg attcagtgca cagggactgg tacagcttat ccgtgacagc ctgcctgagc
    52981 agagaaagga cgaagccgtg gggatcattc tgagtaatga cccggaccac acccgcaacc
    53041 tgtttgatgt gatgtacggg gccagaaaac ccgtcacacc ggaaaagaac ttcatggttt
    53101 ccgtcctctg tgcattgtgt gtggataccg gcaccggtca gccctgtaat cccggcgaca
    53161 cccggcaaat catcagcagc ctggtggacc tggccttccg ggaatatggc gagaataacc
    53221 ccagacttta ccgcgcaggt acggaacccc tggtggatct cgccctggaa gagagcggca
    53281 tcgctgaaca acatgatgcc ggatggtgga atgccgccac ctggtttgaa atcagggaca
    53341 tgctgcatat tgcagggaat atccccgccg cccagcgggc acactaccag gccatgcccc
    53401 tgctggctga aatgtccgcc ctgctgggac agccgtccat ccgggatgtg ttcggcaccg
    53461 ttcagcgtga taactcggaa gagcggttgc tggactacat ccgccgtgcc ctcgatcagg
    53521 gacacagtga ctacccgatg atgtccggct gtacccggtt tatgctcagc cccgacaccc
    53581 gcgtggtcgc cgttgatctg aacaatgtgg ccggggacaa gacacctgcc ggacgtctgc
    53641 gcaccggtat catgtatctg ctggccgggc agatcgccgg cggggatttt gtcctgccgc
    53701 agtatcagga ggaaatacgg aaaaatctcg acccacgcta ccatgaggtg attttccgcc
    53761 gcattgaaca gcttgatcag gaggtcaaga ccaaagtcta tgacgaactg cacaatgcaa
    53821 aagggatcaa cttcatctgg gaagccctgg acacccagga gctggagcag cgtaagttcg
    53881 ggatacgcac ggtgctgagc acccagctgc tgcagcatta tcctccggcc gtgctcaaat
    53941 ccgccaacac cctgtggctg ctgcgttacc gccctgacga aattccgttc ctgcgggata
    54001 actttggcgt cccggaagtc acactgcggc gcttcctgaa aatgccggaa ggggccgcac
    54061 cggacggcag cggtgtcccg gtactggctg tgttccgtgt aaaaaacggc acgctggccc
    54121 ggatcctgaa attcaccctc ggtccactgg agctgtgggc actgaactcc tctcctaaag
    54181 acagcgccct gcgacgtgcg ctgacgcagg aggtggggtc actgcgggca cgccagatcc
    54241 tggccgaaca ctttccccgg ggttcagcca cctcactgat agaacaccgc gcccgcacac
    54301 atgacagtga aaacgtcatc catgagctgg cagcagaact catccgtaag cagggatata
    54361 acctctgatg tacagcattc atatcacccc ccgctgccgg cgtcctgccc ggggacaggt
    54421 cttcctgaaa acactgcgcg tggacggtga cagtttcagc ttcaccctgc cgtccgcctg
    54481 cttcacactg cgaccgtccc ccaatccccg ggtcattttt gccagtgaca gacgggctga
    54541 cggggagatg agtggtattc gcctggccct gcccggagag acaggcacgc tgataacaac
    54601 ggccacctgg caggttaacg tgccggagcg accgcctttt gatatacagc atgtcattca
    54661 ctggcttctg cccgcaccac tcactgcgct gatgtctgac aacacctggc tgtggccgga
    54721 tgtccccggc acaccggcac tcacagccgg agactggccg gtcatggaaa gcgacacccc
    54781 gacactgaaa cgcgtgaagc gtcgtacctt cacgctcatc gataaccgta accgtcaggg
    54841 atggatcacg cgccgcttcc agtgtttccc gctggctccg ctgccggaat cactcagtca
    54901 tcgcgtacca gacatcctgt atcccccgaa aacggagaaa aaagaatgca aagacaatct
    54961 gcaaccacac tgcttgcact ccttgtaggc tgtgcggtta tccctgccac ccatgcctac
    55021 tctgtgaatg ttgtgtccag tcagccggtc acccgacaga tcatgccgga gctggccacc
    55081 atacagggca tcatggagga aatggtccgt atgcagacgg ccaccggcac agccatcacc
    55141 cagaactcag aaaaactggc caccgtcatt gcccaggacg ggcaggccac ccgtcagcag
    55201 atgatattca gtaatgaaac acaccgcctc gaggaggcca ggaaaagctt cagcgtgccg
    55261 gactccatct gcagtgaatc cgcgtccggc attgcagcag aaagccgccg ggcagcagcg
    55321 tctgcagcgg cacgtctgag tcagggggga ggggtcagca gtaaacccat ccgggaacgt
    55381 ctctcccggg ctgcggactc cccggtccgc gaggcgtatg atagtgccgg catccacgcc
    55441 ggttactgca cggaagcgga gtatgcccgc tttggcggaa ctgatgtctg ccctgccgtg
    55501 ggcgatctgc cgggcggaga cagccaggta cgatcgctct atcagggcgc aggcacagca
    55561 gataccccgg ccgctctcac ctgggatcag aaacagattg atgccgcgac ggcttatatg
    55621 aaaaacaccg ccagaccgtc agcgggtcgt gccccgggga aaggagaagt gggcacacag
    55681 accgggcgca cgtatgtggg cctgcagaat gaatacaacg gcatcatcga tgccgcctct
    55741 catccgcagc tgtcactgat tgctgacagt acccctaatg aagccacccg tggggcactg
    55801 actgaagccc tgcagtcacc gtcagctgcg gcatattttg accggaccgc atcatcagaa
    55861 gcccgcaccc gtggccacat gagccagcgg gagtttgagg cctttgaagc cggcagacgg
    55921 tatgccaaca ccgactggca gcaggatctg cagggcatgg aaggcgataa ccttctccgt
    55981 gagctgctgc gcaccaccgc cctgctgaac tggcagatga acgatctgaa agagcagatt
    56041 cgtcagggta acgttatcgc cgggcagcaa cttgcgctgg ccgcacggca gtattacgga
    56101 cagcgccttg gcgaactgtc acaggccatg agccagggga gcgtaagatg aaagacgaca
    56161 acagcatcga cgaaaaaaaa ccggcccccc gtctccgccg catcggtgat atagggataa
    56221 atctgttcgc accactggcc gaaacccgct ggatgctgcg cagctgccgc catatgctgg
    56281 accagcataa aacgcgcctg aagcgcctca tgcccgaccg gcaaccggag agtccggtct
    56341 gtatgacctg ggaggaagcg gtcagggcca gcggactgac ggtcgacgaa ctggaccggc
    56401 gtctgcgacg tcgcaggatg ttctggcgga caggattctg gctgatgatg ataccggtac
    56461 ttctgctgtg tggtatggcc cttgcggcca ccgcactgcc ggtcatcacc ctgatacgta
    56521 tcctggtctg cctgttactg atgagtacca ttgcagcact gtgtgcctca aaagccctga
    56581 ttgcgtcata ccgcctctgg cagctgaatg aacgtcgggt cagcgaggac gaacagggca
    56641 cattccccga ttacctgcag gcccggcacg gctggaaaca ggccacatgg ctggccatta
    56701 cccaccggca gtactgactg tccggtctgc aatttctttt cctcattccg ggggctgatg
    56761 cccgggcatc cccttcggtt ccggagttac cgtgaaaatc atacccagag cactgagtgc
    56821 aggtctggcg ctggctgccg tacctgcaca ggctttcgtc acctatcagg acattgtgag
    56881 tgccgccacc aatccggatg acctgtcgcg ccaggcactg gtcaccattt ttggtgatgt
    56941 ggtcacaaat cccctgacag ccagcagcaa caccatcatt ggcaacctgt tcatggtgtt
    57001 taacgcaatt atcgccacga tggggatagt ctggtttctg tttattggta tccgtcatgc
    57061 tgtccgcacg ggacaccagg gcagtgtctt taacaacgga cgggacgtcg tcggcatact
    57121 cagtgtcgtg agtggatttt tgatgattgt gcccaccgct tccggctgga gtctggcgca
    57181 actggtcatg ctgtggggcg catccatcat gggcatcggt tccgccaata tcatggttca
    57241 gacagcggca gaaaacattg ctgaaggcta ttcaatgacc gtacagccag tacatgtctc
    57301 cacccgcacg gcagcacggg gaattctgga gatggaactg tgcaaacacg ccatgaatac
    57361 gggcctcgat gactttaacc gtaccgcacg ttcatcaacg ccgcccatga gcgaatcctc
    57421 acggacagag aatggcagat ataccgtcac cgtctcaaat ggcagcgcca cctgcgggag
    57481 tgtgagtctc tccgtgaaag gaaacggcag taacaagcag tccgcgctgg cccatttttt
    57541 caatcccttc attgaaacgg aatatcaggg ggttctgacg gcacagcgct ccgccatgga
    57601 taccatgctc cgtgacctgg acagcgcggc gcaggaattt gtgtcggcat accggacccg
    57661 gcgcgacacg ggtaacggca acctgccgga tattgaaagc cgtattcaga aggcagccaa
    57721 ccggtatgaa gaaacggtgc agaaggcgtt gccaacagat aacgcagaaa ctgagcgcag
    57781 ggagcagctg aaatcctacc tgaccaccta cggctgggtt gccctgggcg cctggtacca
    57841 gacctttgcc accgccaacc agcgactggc tgaactggcc gaccgtgctc ctacagtgag
    57901 tggcgtctcg tcactgggcg aagccgggga tacggaattc ctgaaggaag tcaagggggc
    57961 ttaccaggca cagctacaga actcagcgta tactcctgca ctgggaacaa ccacctctgc
    58021 cagtgaatac cacgcatcta atactcaaaa tcctcaatca gccctgttag agttcatagg
    58081 gccagggtta actcgatttt caaataaagt agcaaccata cattcaggaa caggaacaac
    58141 gagtcagcag gcaaatcctc tgctgaagat gaaggacatt ggtgattaca ccatggtcac
    58201 aaccgaaacc atctgggcgg gttataccac cgtcagggtg ctttctactg ccggcagcaa
    58261 ctccgttttt ggccgactgg tcaatgccgt cagcggctta tctgatgcag caaaggcttt
    58321 tctggaagcc tccgcaccac cactctactt cctgctgttt ttgctgttct gtgccggttt
    58381 ttcgttatct atttacctgc cgtttattcc gtttatcttc tggatgaccg gcatcggcaa
    58441 ctggatagtc agcgtactga ttggatgtgc tgctggcccc ctgtgggccg ccacccatct
    58501 gggaacaagt caggacaggg gaagccgggc cgcttacggt tacatctacc tgatagacag
    58561 catgatcaga ccgctgctga tggtattcgg gttctttttt gcatcggtcg ccatcgtggc
    58621 cggcggcaca atactgaatg cgctgtttgc tgctgcgctg atgaatgtcc agataaactc
    58681 tgtgacgggc gtgttcagca tggcgggatt tttgatgatt tatgcccgag cctgtaccac
    58741 gctggttgcg gccatttttg cacttcaggc ttatctgcct gaccatgtaa ttaattttct
    58801 gggcgggcgt gacggtgtta atatgctggg aaatatggtt tcttcagtca aagatattat
    58861 tgccggaggt aataccaata tgcgcagaac acctggcgtt cgggaagata gactgaaaaa
    58921 tattaataga gatgataaag acggaataaa acaataaaat gaaaacatag aaaaaccaat
    58981 aaaacagcat tgccatattc acctgaagag tttaaaatat attcaggtga atatggaggt
    59041 ttattatgaa caaaacaaaa agcagatatc cttcctggtg ggcatattac cacttggccc
    59101 gcaacgctta tttcttatta ggaataccgt gtttactttt atactcagcc gcttatacat
    59161 cagatttatt agatgataat ctaagacatt caataagtct ttttattctg gcatatggat
    59221 gttattttct tcttttcatt cctccgctct ggttatatct gagaaccaga gcgaagaaaa
    59281 agaaagtcca gcaggttgtt cagcagataa aagaaagcag tccctttgcg ccgaccagcg
    59341 attacgagca actgtcattc tcaaagagtt gttatttcgg gatcgacatt aaaaacggca
    59401 ccatgctgta tgtgcgcatt tatccgaaca atgtgatgga tgtgattggt ctggatattc
    59461 acaactttac ccgcacggtt gctgaggacg gcaagctgga aatccacacc acctatgtga
    59521 gtctgccgat gattcctctt gagatctccg gcatctcagc aaggacgttg gccaacacca
    59581 tgcacaccat ggctgcacgt ggatatgagt acaaggaacg tttcccacag atgatccggt
    59641 atcgggttaa agagtgggaa aaagttgccg gtgttcctgt tgcagaagta ttttgaggta
    59701 gctatcaact gttagacgat aatccgtcag ccggtttctc tgaaccggct ttttcttttc
    59761 aggcagaatg cctgtctgcc gtctatctca ggacgccgca tggagtctct atgttttccc
    59821 cacaaattct tagcactgtc ttgcccactg tagacaaaac acagtatcgt tacacagacg
    59881 acgattaaca tcattaaaaa cgttcgttgt ggcatcgcag ccactccatt gcctttcggc
    59941 cgtcatcggg ctactataat gttgtctagg cattacagtg ggccctgggt tgattgataa
    60001 aaaaatcgcc tggggctttc tgcttcatgc cgtccacaaa acgctttcgg catacagcct
    60061 caagcgcccg ccgctattct aatcgttttc ctgtccgcca caatcctttt catgccacca
    60121 cgtattttgt gtcagggaca gaaataaagc cggaaagtga ctacgtgtgc acacatctgt
    60181 tttccgggct gcaggcatcg tgttattttc tgaaatgcaa aataataaaa aacaaggaca
    60241 catcatgaag gatgaaatac acagtaccag cattaacccg gatgttgttg agcagtttct
    60301 ggcccttatg aatacacatt gtaaatcctc agaagaggcg ctcaccatgc tgagtacgct
    60361 ttgttacaac ctgatgatga catgcggtat ccattccatg gacagccaga tgctcaatgg
    60421 gcatgggata tccatgcaac ttcaggggat tgatattgcc gaaaagccag gcaagaatat
    60481 tcactaacct gaacagggcc tgaaaacgct gtcttttcag gcctctgcag gcaaaaactg
    60541 atgacaacaa aatttctgcg tttgtacccc accgatgaaa aagacaaaaa ctcacaccgg
    60601 gcttctcatc attaaagata aaacccgacg tgtcagcctc tacgaaacgc caacggcatg
    60661 gtgtatcagg gggcaggagt gctacagcaa aagcactgga cgccgctgtg gcagccacga
    60721 cagtctgagc agactgcgcc tggacagcat taagcctgtt gaatgagtgg tagcaccatg
    60781 aaagataaca acacaaccgc caccccagaa acctggaaaa tgcgttttct ggccaacggg
    60841 acatccggca catggtttta cactgacgac attgcagtca ctgacgcgtg gcttcgcgcg
    60901 cacccggatg atgtggaggt tatccctctc tgccggatac agccccgcca gcattcccat
    60961 gaacaacata ttgaagaact ccgcgcagcc ctggcccgga tgctccatat gcatcatatg
    61021 atgctggaaa ccacacatta ccgggaaaat atcaccgatg cccgttgtat ccgtgaaatg
    61081 catgaagcgc cattacaggc gctcaatatt ctgtccagat acccgcataa aaaggacgag
    61141 gtgtgacgca tgtggctact gattttctgg ctttcatcgc cctttaatgt ctcttccggt
    61201 gatgcccgtc agcccacact ggcggtgcac agccaggcct ttcataccga acgggcctgc
    61261 cgcgatgcat tgcgcgccat taaaaaggag aatgttggcg aattttctct gcggggcgtt
    61321 tgtgtttctg acggaaatac gaaggaggag gctgagagct catgacggat attactgaca
    61381 gacatacact tcgccgtatg tcctggtcgg aactcatcac ggcaggacag gaagcagaat
    61441 ttcagcatga ttacgagcgt gccagaatag tgtggtcatt cgccttacat gttgcaacaa
    61501 ccacgattaa caaaaatctg gccatagctc atattcgacg ctgcgatact ttgttgcata
    61561 aatcaaaggc tgtacccgga aacaacccgt gtggttccgg tcagcgagtc tgagacctca
    61621 acatcccggg tgatgattgt cattccggac tgacgcagcc gctgcacatc tgcggcaata
    61681 cgttgaatac caagccagaa cagaccatcc agcgcggtga caattaaccc gctttccagc
    61741 gccagtttca gccgtgctgt cggggcttta acctgcccgg ctatatcagt gaaacaacgc
    61801 gtcgtcatgc catagctgtc cgcccgccgt attcgcgtcg caagactgta actgaagcta
    61861 tccggcacgc cggataagtc attctgcaga ctgtatacac atccccgctg ccgctcattg
    61921 aatacaactt ttttctggct gagctgccat tcccgccgca cgctgtcaat catctgcggt
    61981 gtccgggtta acagcagacg aaacggcagt tcaaaactgg tcacataatc aacattaagt
    62041 aaggcaatcc gcagccgctc cctggctcct gctccggtat cacggcattc acgcatgaca
    62101 tcttcccact ggcgcatcag tcgcggtgac tcattcctgg ccctgaactg atatttctca
    62161 atctgataat caatatgttc ctgcataccg tcaccggata tctgttgccg tatcatcacg
    62221 gatatcagcg tactggaatt catgccctgc gtgaagtatc acactgccaa aaaaaacaca
    62281 cctgaacgtt attatttcgc aatattaatt gcacctgttt tcataaataa aaaggagacg
    62341 atgaaatgat aaaatatata ttttcactca ccattatttt ttccgcctct ctctgtgccg
    62401 cacagattca ggggcacgtc atccgcgttc ttgatggtga tacgctggaa atattacagg
    62461 acaaaaagcc tgtcagggtc cgtctggcta atattgatgc accggaaaag aaacagccct
    62521 ttggacgctg gtcagccact cagctgaagg acctggtggc cggaaagccc gtaaccgtgc
    62581 aatacgcgca gacagacagg tatggccgta ttctcggccg ggtctatacc gctgacggca
    62641 cagaagcaaa tcgctggcag gtccgtaacg gcgctgcctg ggtgtacccg gactacaata
    62701 atgactatac tctcccgccg ctccagcgtg atgcccgcgc cctgaagcgg ggattatggg
    62761 cagataaaaa tccggtacca ccatggacct ggagaaaaca gaataagaaa taattatttg
    62821 ctgatatgaa gaaaaaaacc atttcaaaga aaataaatgg caaaaataca gcatggaaaa
    62881 aattgccatt tttaccgccg cgttattttt aatatttgct gtctgctggt cgtaataagc
    62941 agaataacag aaaggtgtaa aaatgaacct ggttgatgca tttgtcacta aagtgatatc
    63001 cggaccgtat gaagaatacg gaaaatggtg ggtggatgta gaatatattt cctggggagt
    63061 tccgggtaaa agccggctca tgtttgactc acgggaacag gcacaggaag tgcaggaagg
    63121 ttttcgattt ttaacctgag catattttgt taaagggcag aaggcggtga ccctctgccc
    63181 ttaagtacgt ttccgggatt atcagtcaac cagttcccgg atcagttcca ggattgctat
    63241 cactgcgtta gccacagcaa caaccgccct gagaacgatg attaacaggt taatcatccg
    63301 cttctccatc aatggagaaa gaacaccgtg gtttgccctt acattgcctg tcgccacgtt
    63361 ttctatagtg tcagttatag acagttcttc tccggccaga gcaccaaaac cagcaccacc
    63421 tgatgttttg gccaggactt gcaacctgtt catcccggtg aacgatatgc cggaaagaac
    63481 gtgcctcaaa gctgacacac cgagtatata cttatactgt gcttgcagaa atattatttc
    63541 agccgtatta tttcatagtc agttataggc agttatcttc cggtcagggc taccaacttc
    63601 gatccggaat cgcaaaaaaa ccggcctcac cagtcggttt ttttgcgtct gtacctcctc
    63661 cccggacagc agttctctca ctcccgttga atatttcctg ttgaatttta cgaaaatacg
    63721 gcaaatggga gatatcgaat atatttactt gggtgtagtc ggtaaaaacc agctcatgtt
    63781 tgactcacag aaacaggctc tggaagtaca gaaagtaatc tgtccgtaga ttattaaagg
    63841 gcagagggcg gtgaccctct gcccttaagt acgtttccgg gattatcagt caaccagttc
    63901 ccggatcagt tccaggattg ctatcactgc gttagccaca gcaacaaccg ccctgagaac
    63961 gatgattaac aggttaatca tccgtttctc catcaatgga ggaaatcact gttgtttact
    64021 cttacattgc ctgtcacaac agctctttca ttgttagctg aaaagagact tcctccggcc
    64081 agagcaccag acgcagcacc acctgtttgt ccggccaggt ctttgaatag cacagatcca
    64141 tagtcccgat ggattgatgc gaaggtaagt ctccagacaa ggctaacttg ctgattatac
    64201 ttgtttcagg cgatatcgct actcccggaa caatagactg taacgaacag attgcgatca
    64261 ggtgaatata acgatattct ggtaacgtta gttgaaaaga gacttcctcc gggccagagc
    64321 cacccactct ccctgggatc gcaaaaaagc cggcctcacc agtcggcttt tttgtgcctg
    64381 tctctcacca ccttctgctg ttcctgtgaa aatgccctgc tgaattcagc cctgcccagt
    64441 cattaccggc gattgttcgc attaattcca ctcagcctca tcccgaaatt tatctattta
    64501 tcgatggagg aatccccgtg tcttaccaac agacgtctgc cgccgaagat ccaatggcta
    64561 tatggtatat cgtcggtgcc atatgcctgc tctttgcgat tatcatctgg cgctttttac
    64621 ctgaaatcgt cttcgcctcc tgcctgatcc tgcacacact gtggggcatg attgactggg
    64681 ggccctttca caactttgcc gccccccgtt acaacctgct ggccatcact gctaacaacg
    64741 cggcaaccat cactttcggc cagtggctgg atgttatgtc ccggacagtg gggatcctct
    64801 ggttgattct gcttcccatg acattcggct ttctgtggat gtggtttcac catccggccc
    64861 agccccgttt tacccgcagg ccactgaata tccacacact gccccatatt ttttcagctc
    64921 tgtcaccggc gatcgccccc gttctggctg atggagacaa caacagactc ttccacggac
    64981 aaaaacgtcc cgagcgccgt gtcgcactaa cccccgaagt ctttgtggag cagaacaacc
    65041 ttatccgcaa catgcagctc gatgttgcgt ccacacgcca gtgcttcatg gcgcaactgg
    65101 ggcaaccact gaccagctgg aaagacatgg ccccacatga aaaagcgctc tttgccatct
    65161 ttggcctgca gttcttcctc ggtgaccgga aagcagcggt agccctgatg aacaacctca
    65221 atctctcctg caggctcaaa agtaaacggg accaggggcg tttcagcacg ccggtgtatt
    65281 cactggccag aaacgccttt atccgggtca tcaaaacaga aggcgcacaa cagtggctca
    65341 ggcagcaccg ttacgttcgc agcggtctgg tctggcttta cgcacatgat ttgcgcctga
    65401 cgcctccgaa ctggctgtgg ctcaagggcg ttgaccggac gctcttctat gcccttcacc
    65461 gggcaaacac cacaaaaggc ttcatcgaag gggccggcgt ggtggccgtt gcccgggcag
    65521 aaaatgaagc cagccgtctc ggcctcccct gcccggaacc ctgtgtggaa gaggccattg
    65581 aagggcttcg acgggacatg ctggggctgg gcctgatatg ggacgaacct cagccagatc
    65641 gcgatcgtaa acgtcagatc aggacccgct ggtcactcac ggatgacgtg ataccccgcc
    65701 gccatgataa cgacgaagac actgacactg g
//
LOCUS       DAZGJT010000013        65166 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZGJT010000013 DAZGJT010000000
VERSION     DAZGJT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65166
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.013"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..504)
                     /gene="folK"
                     /locus_tag="ACHKFK_000933"
     CDS             complement(25..504)
                     /gene="folK"
                     /locus_tag="ACHKFK_000933"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO9051334.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALKTTLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELVFPDGLSLVEALQAKGFNELDKW"
     gene            complement(501..1898)
                     /gene="pcnB"
                     /locus_tag="ACHKFK_000934"
     CDS             complement(501..1898)
                     /gene="pcnB"
                     /locus_tag="ACHKFK_000934"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO9051335.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1958..2854)
                     /gene="gluQRS"
                     /locus_tag="ACHKFK_000935"
     CDS             complement(1958..2854)
                     /gene="gluQRS"
                     /locus_tag="ACHKFK_000935"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO9051336.1"
                     /translation="MTDTHYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLYWDGDVLWQSQRHHAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRDLHHGPDNAAVRIRQQHPVTQFTDLLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALHFLGQQVETHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2921..3376)
                     /gene="dksA"
                     /locus_tag="ACHKFK_000936"
     CDS             complement(2921..3376)
                     /gene="dksA"
                     /locus_tag="ACHKFK_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO9051337.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3554..4258)
                     /gene="sfsA"
                     /locus_tag="ACHKFK_000937"
     CDS             complement(3554..4258)
                     /gene="sfsA"
                     /locus_tag="ACHKFK_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO9051338.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAIICVNTLWANRLTKEAILNESISELA
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     LSAEGMALKKSLPVTL"
     gene            complement(4273..4803)
                     /gene="thpR"
                     /locus_tag="ACHKFK_000938"
     CDS             complement(4273..4803)
                     /gene="thpR"
                     /locus_tag="ACHKFK_000938"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO9051339.1"
                     /translation="MSEPQRLFFAIDLPAEICEQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYTVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4877..7306
                     /gene="hrpB"
                     /locus_tag="ACHKFK_000939"
     CDS             4877..7306
                     /gene="hrpB"
                     /locus_tag="ACHKFK_000939"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO9051340.1"
                     /translation="MSSLPVAAVLPELLAALDGASQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQTLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLTSRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWMDQPPTVNLLAAKRL
                     LRMLGALDGERLSAQGQKMATLGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAYADRIARRRGQEG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLMVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWVMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7400..9928
                     /gene="mrcB"
                     /locus_tag="ACHKFK_000940"
     CDS             7400..9928
                     /gene="mrcB"
                     /locus_tag="ACHKFK_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO9051341.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGRKPRGKRGWLWLLLKLAIVFAVMIAIYGVYLDQKIRSRIDGKVWQLPA
                     AVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFDFP
                     DSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPRSG
                     FPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNLFL
                     SSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYYFG
                     RPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYDML
                     SARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAAEK
                     AAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSIGS
                     LAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLVDA
                     LTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLNPVPAMLLGALNLTPIEVAQAFQ
                     TIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGTGR
                     QLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAMSI
                     YQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSEMQ
                     QQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10148..12391
                     /gene="fhuA"
                     /locus_tag="ACHKFK_000941"
                     /pseudo
     CDS             10148..12391
                     /gene="fhuA"
                     /locus_tag="ACHKFK_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308181.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
     gene            12442..13239
                     /gene="fhuC"
                     /locus_tag="ACHKFK_000942"
     CDS             12442..13239
                     /gene="fhuC"
                     /locus_tag="ACHKFK_000942"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO9051342.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAAEREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13239..14129
                     /gene="fhuD"
                     /locus_tag="ACHKFK_000943"
     CDS             13239..14129
                     /gene="fhuD"
                     /locus_tag="ACHKFK_000943"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO9051343.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAVIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAVYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRILDNAIGGKA"
     gene            14126..16108
                     /gene="fhuB"
                     /locus_tag="ACHKFK_000944"
     CDS             14126..16108
                     /gene="fhuB"
                     /locus_tag="ACHKFK_000944"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO9051344.1"
                     /translation="MSKRIALFPVLLLALLVVAAAALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLVKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16143..17423)
                     /gene="hemL"
                     /locus_tag="ACHKFK_000945"
     CDS             complement(16143..17423)
                     /gene="hemL"
                     /locus_tag="ACHKFK_000945"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO9051345.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LREAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAAHRVFAKL"
     gene            17589..17636
                     /gene="yadX"
                     /locus_tag="ACHKFK_000946"
     CDS             17589..17636
                     /gene="yadX"
                     /locus_tag="ACHKFK_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO9051346.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17648..19069
                     /gene="clcA"
                     /locus_tag="ACHKFK_000947"
     CDS             17648..19069
                     /gene="clcA"
                     /locus_tag="ACHKFK_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO9051347.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDVFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVVMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASARENT"
     gene            19093..19158
                     /gene="yadW"
                     /locus_tag="ACHKFK_000948"
     CDS             19093..19158
                     /gene="yadW"
                     /locus_tag="ACHKFK_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO9051348.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19151..19495
                     /gene="erpA"
                     /locus_tag="ACHKFK_000949"
     CDS             19151..19495
                     /gene="erpA"
                     /locus_tag="ACHKFK_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO9051349.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19542..20165)
                     /gene="yadS"
                     /locus_tag="ACHKFK_000950"
     CDS             complement(19542..20165)
                     /gene="yadS"
                     /locus_tag="ACHKFK_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO9051350.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20203..21003)
                     /gene="btuF"
                     /locus_tag="ACHKFK_000951"
     CDS             complement(20203..21003)
                     /gene="btuF"
                     /locus_tag="ACHKFK_000951"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO9051351.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANVLRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(20996..21694)
                     /gene="mtnN"
                     /locus_tag="ACHKFK_000952"
     CDS             complement(20996..21694)
                     /gene="mtnN"
                     /locus_tag="ACHKFK_000952"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO9051352.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21778..23295
                     /gene="dgt"
                     /locus_tag="ACHKFK_000953"
     CDS             21778..23295
                     /gene="dgt"
                     /locus_tag="ACHKFK_000953"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO9051353.1"
                     /translation="MTQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDVESQPLTDDRCSVAAL
                     RLRDGEEPLNALRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLNLPLSDFTELVEKERVKRFPIETRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23425..24849
                     /gene="degP"
                     /locus_tag="ACHKFK_000954"
     CDS             23425..24849
                     /gene="degP"
                     /locus_tag="ACHKFK_000954"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO9051354.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGLGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSDLAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSST
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25004..26161
                     /gene="cdaR"
                     /locus_tag="ACHKFK_000955"
     CDS             25004..26161
                     /gene="cdaR"
                     /locus_tag="ACHKFK_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO9051355.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26214..26600)
                     /gene="yaeH"
                     /locus_tag="ACHKFK_000956"
     CDS             complement(26214..26600)
                     /gene="yaeH"
                     /locus_tag="ACHKFK_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO9051356.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26919..27743)
                     /gene="dapD"
                     /locus_tag="ACHKFK_000957"
     CDS             complement(26919..27743)
                     /gene="dapD"
                     /locus_tag="ACHKFK_000957"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO9051357.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFANYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27774..30446)
                     /gene="glnD"
                     /locus_tag="ACHKFK_000958"
     CDS             complement(27774..30446)
                     /gene="glnD"
                     /locus_tag="ACHKFK_000958"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO9051358.1"
                     /translation="MNTLPEQYANTALPTLHGQPQNPCAWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASKETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLHALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30508..31302)
                     /gene="map"
                     /locus_tag="ACHKFK_000959"
     CDS             complement(30508..31302)
                     /gene="map"
                     /locus_tag="ACHKFK_000959"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO9051359.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31670..32395
                     /gene="rpsB"
                     /locus_tag="ACHKFK_000960"
     CDS             31670..32395
                     /gene="rpsB"
                     /locus_tag="ACHKFK_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO9051360.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32530..33381
                     /gene="tsf"
                     /locus_tag="ACHKFK_000961"
     CDS             32530..33381
                     /gene="tsf"
                     /locus_tag="ACHKFK_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO9051361.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33528..34253
                     /gene="pyrH"
                     /locus_tag="ACHKFK_000962"
     CDS             33528..34253
                     /gene="pyrH"
                     /locus_tag="ACHKFK_000962"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO9051362.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITK"
     gene            34403..34960
                     /gene="frr"
                     /locus_tag="ACHKFK_000963"
     CDS             34403..34960
                     /gene="frr"
                     /locus_tag="ACHKFK_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO9051363.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35052..36248
                     /gene="ispC"
                     /locus_tag="ACHKFK_000964"
     CDS             35052..36248
                     /gene="ispC"
                     /locus_tag="ACHKFK_000964"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO9051364.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36437..37195
                     /gene="ispU"
                     /locus_tag="ACHKFK_000965"
                     /gene_synonym="uppS"
     CDS             36437..37195
                     /gene="ispU"
                     /locus_tag="ACHKFK_000965"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO9051365.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37208..38065
                     /gene="cdsA"
                     /locus_tag="ACHKFK_000966"
     CDS             37208..38065
                     /gene="cdsA"
                     /locus_tag="ACHKFK_000966"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO9051366.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRIIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWVNLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38077..39429
                     /gene="rseP"
                     /locus_tag="ACHKFK_000967"
     CDS             38077..39429
                     /gene="rseP"
                     /locus_tag="ACHKFK_000967"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO9051367.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGKGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39459..41891
                     /gene="bamA"
                     /locus_tag="ACHKFK_000968"
     CDS             39459..41891
                     /gene="bamA"
                     /locus_tag="ACHKFK_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO9051368.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42013..42498
                     /gene="skp"
                     /locus_tag="ACHKFK_000969"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42013..42498
                     /gene="skp"
                     /locus_tag="ACHKFK_000969"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO9051369.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42502..43527
                     /gene="lpxD"
                     /locus_tag="ACHKFK_000970"
     CDS             42502..43527
                     /gene="lpxD"
                     /locus_tag="ACHKFK_000970"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO9051370.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43632..44087
                     /gene="fabZ"
                     /locus_tag="ACHKFK_000971"
     CDS             43632..44087
                     /gene="fabZ"
                     /locus_tag="ACHKFK_000971"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO9051371.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44091..44879
                     /gene="lpxA"
                     /locus_tag="ACHKFK_000972"
     CDS             44091..44879
                     /gene="lpxA"
                     /locus_tag="ACHKFK_000972"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO9051372.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            44879..46027
                     /gene="lpxB"
                     /locus_tag="ACHKFK_000973"
     CDS             44879..46027
                     /gene="lpxB"
                     /locus_tag="ACHKFK_000973"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO9051373.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAAVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46024..46620
                     /gene="rnhB"
                     /locus_tag="ACHKFK_000974"
     CDS             46024..46620
                     /gene="rnhB"
                     /locus_tag="ACHKFK_000974"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO9051374.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPSMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46657..50139
                     /gene="dnaE"
                     /locus_tag="ACHKFK_000975"
     CDS             46657..50139
                     /gene="dnaE"
                     /locus_tag="ACHKFK_000975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO9051375.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGTAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAESRGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLDTELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50152..51111
                     /gene="accA"
                     /locus_tag="ACHKFK_000976"
     CDS             50152..51111
                     /gene="accA"
                     /locus_tag="ACHKFK_000976"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO9051376.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51209..53350
                     /gene="ldcC"
                     /locus_tag="ACHKFK_000977"
     CDS             51209..53350
                     /gene="ldcC"
                     /locus_tag="ACHKFK_000977"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO9051377.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RGEFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFSDADANHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTEESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKHDEDGVYRVRVLKMAG"
     gene            53407..53796
                     /gene="yaeR"
                     /locus_tag="ACHKFK_000978"
     CDS             53407..53796
                     /gene="yaeR"
                     /locus_tag="ACHKFK_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9051378.1"
                     /translation="MLGLKQVHHIAIIATDYAMSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            53861..55174
                     /gene="tilS"
                     /locus_tag="ACHKFK_000979"
     CDS             53861..55174
                     /gene="tilS"
                     /locus_tag="ACHKFK_000979"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO9051379.1"
                     /translation="MTLTLNRQLLSSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVKHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     HTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTQLIRPLLARTR
                     GELVQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTPQGTLQIALMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVAGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAVGENGVSFVWQKTLSQVKAG"
     gene            complement(55208..55468)
                     /gene="rof"
                     /locus_tag="ACHKFK_000980"
     CDS             complement(55208..55468)
                     /gene="rof"
                     /locus_tag="ACHKFK_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO9051380.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGATRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55455..55655)
                     /gene="yaeP"
                     /locus_tag="ACHKFK_000981"
     CDS             complement(55455..55655)
                     /gene="yaeP"
                     /locus_tag="ACHKFK_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO9051381.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            55821..56366
                     /gene="yaeQ"
                     /locus_tag="ACHKFK_000982"
     CDS             55821..56366
                     /gene="yaeQ"
                     /locus_tag="ACHKFK_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO9051382.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFAYNSRAAQIWWQQNQSKCAQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTVWQQPS"
     gene            56363..56785
                     /gene="arfB"
                     /locus_tag="ACHKFK_000983"
     CDS             56363..56785
                     /gene="arfB"
                     /locus_tag="ACHKFK_000983"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO9051383.1"
                     /translation="MIVISRHVAISDGEFEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRNQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            56799..57509
                     /gene="nlpE"
                     /locus_tag="ACHKFK_000984"
                     /gene_synonym="cutF"
     CDS             56799..57509
                     /gene="nlpE"
                     /locus_tag="ACHKFK_000984"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591565.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO9051384.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARSNSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNQDCSSLGL"
     gene            complement(57665..58489)
                     /gene="yaeF"
                     /locus_tag="ACHKFK_000985"
     CDS             complement(57665..58489)
                     /gene="yaeF"
                     /locus_tag="ACHKFK_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFO9051385.1"
                     /translation="MDKPKAYCRLLLPCFLLLSACTVDISQPDSSATVVNAEAKTWAV
                     KFQHPSSFTEQSIKEITEPDLKPGDLLFSSSLGVTSFDIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAIKHSDKLFVLRVPDLTPQQATEITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKPQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDISAFKPETQLQYVGHMKPGIYIKAGRFVGLTQ"
     gene            complement(58542..60260)
                     /gene="proS"
                     /locus_tag="ACHKFK_000986"
     CDS             complement(58542..60260)
                     /gene="proS"
                     /locus_tag="ACHKFK_000986"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO9051386.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVLMPVVQPSELWQESGRWEQYGPELLRIADRGDR
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFLVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVDYL
                     VKQIKG"
     gene            complement(60371..61078)
                     /gene="tsaA"
                     /locus_tag="ACHKFK_000987"
     CDS             complement(60371..61078)
                     /gene="tsaA"
                     /locus_tag="ACHKFK_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO9051387.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVCFTAEVEKQLLTLKKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61075..61479)
                     /gene="rcsF"
                     /locus_tag="ACHKFK_000988"
     CDS             complement(61075..61479)
                     /gene="rcsF"
                     /locus_tag="ACHKFK_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO9051388.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61597..62412)
                     /gene="metQ"
                     /locus_tag="ACHKFK_000989"
     CDS             complement(61597..62412)
                     /gene="metQ"
                     /locus_tag="ACHKFK_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO9051389.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKESPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62452..63105)
                     /gene="metI"
                     /locus_tag="ACHKFK_000990"
     CDS             complement(62452..63105)
                     /gene="metI"
                     /locus_tag="ACHKFK_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO9051390.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTISAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63098..64129)
                     /gene="metN"
                     /locus_tag="ACHKFK_000991"
     CDS             complement(63098..64129)
                     /gene="metN"
                     /locus_tag="ACHKFK_000991"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO9051391.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64317..64889
                     /gene="gmhB"
                     /locus_tag="ACHKFK_000992"
     CDS             64317..64889
                     /gene="gmhB"
                     /locus_tag="ACHKFK_000992"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO9051392.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMFLSARDYLHIDMAASYMVGDKLEDMQAAAAASVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15353 a        16170 c        17966 g        15677 t
ORIGIN      
        1 tatttatatt cacacgaaaa ataattacca cttatctaat tcattaaagc cttttgcctg
       61 tagagcttca actaatgata agccatcagg aaataccaat tccggcgcaa tttcaaacag
      121 tggccacagc ataaagccgc gatttttcat atcgtagtgc ggaacggtca gacgttcggt
      181 atttattact tcattaccaa acagcatgat gtcgagatcc agcgtgcgcg gtccccagcg
      241 ttcagcttta cggacgcgac cttgctgcaa ttcgatacgt tgtgtgtgat tgagtagctc
      301 ttcaggggca agagtggttt tcagcgcgac ggcggcattt aagtaatcgg gttgatcttg
      361 cggccccagc ggtggggtgc ggtaaaacga agaaacagca agaatgcggc tttctggaat
      421 atcgcctaat gctttcaggg cagcattgac ctgctccagc ggagaggcca gattgctgcc
      481 tatggcaata tacgccactg tcatgcggta ccctcacgac gcggtgcgcg tttgcgtgga
      541 cgacgagtac gacgacgcgg tgacggttct tcatccagtt cgttaagcat ccctttttgg
      601 tctggtggcg cggaaacctg gaactcaccc caccatttca ccagacgttg cagttcagcg
      661 ttacgctcaa cttcggctcg taaggccaac aggtcgtatg ccgcacggaa cttaggatgc
      721 tccagcagtt tccatgcgcg ttttccctga cgacgggaca tacgcaactg caactgccag
      781 atatcgcggg ttagcgtggt caaacgtttc gggatcgcca gtgaacggca ggcttcgtcc
      841 agcacgtcgt tcatcgccag tgcgaaagcg tcgtgatagg tcaggccgct ttcctgggcg
      901 atcttctgtg ccgtctccaa caatgggtac cagaacatgg cggcaaacag gaacgccggg
      961 ttcacgcgca tatcgttatg gatacgcgta tcggtattct tcagtacctg ctcaataatg
     1021 cgttccatcg ggctgtcgcc attttccgtg aagtagcggg taatggtcgg gaacagcggc
     1081 tggaacagat gatattcaca cagcagctta taggtttcgt aaccgtagcc cgcttgcaac
     1141 agtttcagcg attcttcaaa cagacgtgcc ggtgggatat cgttcagtaa ggttgccagg
     1201 cgtgggatcg gctcggcggt ttccgggctg atgcgcatgc ccaatttggc ggcaaaacga
     1261 accgcgcgga gcatacgtac cggatcttca cggtagcgcg tttccgggtt accaatcaga
     1321 cggataacgc cgtctttcag atccttcatg ccgccaacgt aatcacggac ggtaaaatct
     1381 gccacgctgt aatacaggct gttgatagtg aaatcgcggc gctgggcgtc ttcttcgatg
     1441 gagccgaaaa tgttgtcgcg cagcaacata ccgttttgcc cgcgttggga ggtcgtgcgg
     1501 tcgctgacgt taccttcgtg atgtccacgg aaggtcgcaa cttcgataat ctccgggcca
     1561 aacatcacat gagccagacg gaaacggcga cccaccaggc ggcagttacg gaacagtttg
     1621 cgcacctgct ctggcgtggc gttggtggtc acgtcaaaat ctttcggctt tttgccaagt
     1681 aacaggtcgc gcacgccgcc gccaaccagc caggcttcgt atcccgcttt attgagcctg
     1741 tacattacct tcagggcatt ttcactgata tctttgcggg aaatagcatg ctgctcacgc
     1801 gggatcaccg tcacctgtgg acgggcgact gcctgttcag cctcgctttc ctcgcggctt
     1861 agcaccttgc ggcaaaaatt agcgactcgg gtaaaaatag tacacctcgg tagtgtcaaa
     1921 catcattcag gacaaaaaaa tagcggctaa tcatagctca gcatgacgca tttgagaatg
     1981 ttgaatttac aattgccgac tcaggcacgg cggtcagcgt ccagtttttg acggctgact
     2041 gaaggatttg ctcgacgctg aaatcctgcc agtgtgtttc tacctgctga cccagaaaat
     2101 gaagtgccgc gattaacacc gggcgcggat cgcctttcgg caacgcaggc gcatgattct
     2161 gcttggaaag tttagcgcct tgtggattaa gcgccagcgg taaatggatg taatctggca
     2221 ctttccagcc aaaaagctgg tacagcgaga tttgccgcac cgtcggttcg atcaaatctg
     2281 ccccgcggac aatttcgctc acgccctgga agtgatcatc caccaccacc gccaggttat
     2341 aggcaaacaa accatcacgg cgatgaataa taaaatcttc ccgtgccagt ttttcgtcgg
     2401 cgtgaataat gcctcgcagc agatcagtaa actgggtgac cggatgctgc tggcggatac
     2461 gtactgcggc gttgtctggt ccatgatgca aatcccggca atgaccgtcg taaataccgc
     2521 caatgctttg aatacgcgca cgcgtgcagg tgcagtaata acttagtcct tgctcatgta
     2581 accaggcgag tgcttcgcga taggcgtggt gacgttgcga ttgccagaga acgtcgccgt
     2641 cccagtacag gccgtaatgt tccagctggc gcaggatagt ttctgcggca ccgggaactt
     2701 cacgaggcgg gtcgatatct tcaatgcgta ccagccagcg accttgccgg gcgcgagcct
     2761 gcaaatagct gccaagcgca gcaatcagag aaccaaaatg aagctcgccg gaaggagagg
     2821 gggcgaagcg gccaatatag tgtgtgtctg tcatctcttt gaacaaaaaa taaggcggga
     2881 gcatttcccg cctgtggtaa acgtgatgga acggctgtaa ttagccagcc atctgttttt
     2941 cgcgaatttc agccagcgtt ttgcagtcga tgcacagatc ggctgtcggg cgcgcttcca
     3001 gacggcgaat accgatttca acaccgcagg attcgcagta gccgaaatct tcgtcttcca
     3061 cttttttcag cgtcttctcg atctttttga tcagcttacg ctcgcgatcg cggttacgca
     3121 gttcgaggct gaactcttct tcctgggctg cacggtctac cgggtccggg aagttggctg
     3181 cttcatcctg catatgtgta acggtgcgat cgacttcatc cctgagttga ttacgccatg
     3241 cttccagaat acgacggaag tgcgccagct gggcttcatt catatactct tcgcccggct
     3301 tctcctgata tggttccacc ccagcgatgg cgagaatact cagggacgat gttttacggt
     3361 tttgcccttc ttgcatgttg cttctcctta acacgcacta tcgatcccca tgttcggggg
     3421 aaaaatgagg ccgctataaa tagcagatgc ttttccggat agcaattatc taaacgtaac
     3481 acttgacaac tgtgtgagga aaagcgtatt tgcgcacgcg accagaatgt aaattaacca
     3541 gttacttact ctactacaat gtaaccggta gtgatttttt aagagccatg ccttcggcag
     3601 aaagttccgc tttgtaagcc agaatttcta cccccctctg ttgagcttct gacaatagtt
     3661 gcgcgtattt ctcatcgatg tggcgcgcgg gtgaaaaccg tgtaatggct gaatgcagca
     3721 cggcgaaaaa gataaccgca cgctggcctt cagccgctac gctcatcaac tcccgaaggt
     3781 gtttctgacc tcgttcagtg accgcatcgg gaaaatatcc ctgttcgttc tccgctaacg
     3841 taaccgattt cacttcaata tagcagtctg gacgcgaatc cgcctgcaac ataaagtcaa
     3901 tacggctgcg ttcggcgccg tattttactt cgcttttcag cgagctatag cctgccagct
     3961 ctgaaattga ttcattaagg atagcctctt tcgtcaacct gttagcccaa agcgtattga
     4021 cgcaaataat tgcgccgctc tggctttgag ttaattccca ggtgtgtggg tatttccgtt
     4081 tggtgttgtc tgaagtcgaa taccagacgg tatcgccagg cgttgcacaa ccggtcatcg
     4141 cacccgtatt cgggcagtgt agcgttaatt cgcgaccatc gggtgtgatc acatcggcta
     4201 aaaaacgttt gtaacgctga attagcgtcg cgcgctgtag agggggagaa aattccattg
     4261 cgacaatcct tgttattgcg ttagcgccca gcgttttagc ggcgtgtagc gtgtgcgtcc
     4321 acgggcaaac gaggaggcgt aaagggtgaa ctccgtcacc gtatacgacc agttaaaacc
     4381 tggcggcggg attgtcaccg cctcgctggc gtcgcgcaat aaggtaatat gtggatgaaa
     4441 cggacgattg ctttgaaaac aaccgctgcg ggcagcctgt gagcggagca tattcgccag
     4501 ctggattaag ccgcgaggcg gctgtcgcat ccctaaccac accacacgcg aacgcagcca
     4561 ttgaccggcg tcatcaagcg tgagtgtgaa accaggttga cgaatccgtc cggctaaaag
     4621 agaaagcgcc ttctctttct ctgcgctcac ttcgcctaaa aatgccagag tcagatgcaa
     4681 attatcggcg gcgaccggac gtcccgcctc aggtgggaag tgtgtggcgc gccagtggat
     4741 aatctgttcg cagatttctg caggtaagtc gatagcaaag aacagccgtt gcggttcaga
     4801 catgtgaggc actcggttat gaattaccgc gatgctacaa tgtggcgcga agaatgttaa
     4861 ccctctggag cgttttgtgt cgtcgttgcc cgttgctgcc gtcttacctg agttacttgc
     4921 tgccctcgat ggtgcatcgc aggtgttatt aagtgcgccg accggggccg ggaaatcaac
     4981 ctggctgccg ctgcaactgc tggcgcatcc cggcattaac gggaaaatta tcctgctgga
     5041 gccgcgtcgt ctggcggcgc gtaacgttgc gcaacgactg gcggagctgc ttaacgaaaa
     5101 gccaggcgat accgttggct accggatgcg tgcgcaaaac tgcgtcgggc cgaatacccg
     5161 cctggaagta gttaccgaag gcgtgctgac gcggatgatc cagcgtgacc cggaactgag
     5221 cggtgttgga ttggtgatcc ttgatgaatt tcatgagcgc agcttgcagg cggatttggc
     5281 gttggcgctg ttactcgatg tgcaacaagg tctgcgtgat gaccttaaac tgctgattat
     5341 gtcggctacg ctggacaacg accgcttgca gcaaacgctg ccagaagcgc ccgtcgtcat
     5401 ctcagaaggg cgctcgtttc cggttgaacg ccgttatttg ccgctgcccg ctcatcagcg
     5461 ttttgacgaa gccgtcgcgg ttgctaccgc cgaaatgctg cgtcaggaaa gtggatcatt
     5521 actgttattt ttacctggcg tcggagaaat tcagcgtgtg caggaacaac tgacttcacg
     5581 catcggcagt gatgtgttgc tctgcccgct gtatggcgcg ttgtcgctga acgatcagcg
     5641 aaaagcgatc ctcccggcac cgcaagggat gcgtaaagtg gtgctggcga ccaatattgc
     5701 tgaaacaagt ttaaccatcg aaggtattcg tctggtggtg gattgtgccc aggaaagggt
     5761 ggcgcgtttt gatccgcgta cagggcttac gcggttgatt actcaacgcg ttagtcaggc
     5821 gtcgatgacg caacgtgccg ggcgtgccgg gcgtctggag ccgggtatct gcctgcattt
     5881 aatcgccaaa gaacaagcag aacgcgctgc cgcacaaagt gaaccggaga tcttacaaag
     5941 cgatctttcc ggtttgctga tggaattact gcaatgggga tgcagcgatc cggcgcagat
     6001 gagctggatg gatcaaccgc caacggtgaa tctactggcc gcgaaacgcc tgttacggat
     6061 gctgggggcg ctggacggtg aacggcttag tgcgcaaggg caaaaaatgg caacgctggg
     6121 taacgatccg cgtttagcgg caatgctggt tagcgcgaag aacgacgacg aagctgctac
     6181 cgcggcaaaa attgccgcca ttctcgaaga gccgccacgg atgggcaata gtgacctggg
     6241 cgtggcgttt tcgcgcaatc aacccgcctg gcagcaacgt agtcagcaac tgttaaaacg
     6301 tttaaacgta cgcggcggtg aggcagacag ttcgcttatc gcgccgctac ttgccggagc
     6361 gtatgccgat cgtattgctc gccgccgtgg gcaagaggga cgctatcaac tggcgaacgg
     6421 catgggggcg atgctcgatg ccgacgatgc gctaagccgc cacgaatggt tgatcgcacc
     6481 gttattattg cagggcagcg cctcgccgga tgcgcggatt ttactggcgc tgccggtcga
     6541 tattgatgag ttagtacaac gctgcccgca gctggtacag cagtctgaca ctgtggagtg
     6601 ggatgacgcg caaggtacgc tgaaagcctg gcgtcggctg caaattggtc agttgatggt
     6661 aaaagtgcaa ccgctggcga aaccctcgga agacgagttg catcaggcga tgcttaacgg
     6721 cattcgtgat aaaggtttaa gcgtgctcaa ctggacggcg gaagcggaac agctacgctt
     6781 gcgtttgtta tgcgccgcaa agtggttgcc ggaatatgac tggccagcgg ttgatgatga
     6841 aagtttgttg gcgacgctgg aaacgtggct actgccgcat atgacaggcg tacattcgct
     6901 acgcggcctg aaatcactcg atatttatca ggcactgcgt ggattacttg attgggtaat
     6961 gcagcaacgt ctggatagtg aattgcctgc gcattacact gtgccgacgg gaagccggat
     7021 cgccattcgt taccatgaag ataacccgcc cgcgctggcg gtgagaatgc aggagatgtt
     7081 tggtgaggcc actaatccga ctatcgccca ggggcgcgta ccgttggtgc tggagttgct
     7141 ttcccctgcc caaaggccgc tgcaaatcac gcgtgatttg agcgccttct ggaaaggagc
     7201 gtaccgtgag gtgcaaaaag agatgaaagg gcgttatccc aaacatgtct ggccggacga
     7261 cccggcaaac accgcaccga cgcgacgaac gaaaaagtat tcgtgaagac caaagcaaat
     7321 ttgagagata tcttcttctg tcttgcaaca gaagaacaga aaatcgggct tttgcgcctg
     7381 aatattgcgg agaaaaagca tggccgggaa tgaccgcgag ccaattggac gcaaagggaa
     7441 accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta
     7501 cgacgattat gatgactatg aggatgaaga accgatgccg cgcaaaggta agggcaaagg
     7561 gcgtaagcct cgtggcaaac gcggctggct atggctgctg ctaaaactgg ctatcgtttt
     7621 tgccgtgatg atcgccattt acggcgttta tctcgatcaa aaaattcgca gccgtattga
     7681 tggcaaggtc tggcaattgc ccgcggcagt ttatggtcga atggtcaacc ttgagccaga
     7741 catgaccatc agcaagaacg agatggtgaa gctgctggag gcgacccagt atcgccaggt
     7801 gtcgaaaatg acccgtcctg gtgagtttac cgtacaggcc aacagcattg agatgatccg
     7861 ccgtccgttt gatttcccgg acagcaaaga agggcaggtg cgcgcgcgtc tgacctttga
     7921 tggcgatcat ctggcgacga tcgtcaatat ggagaacaac cgtcagttcg gtttcttccg
     7981 tcttgatccg cgcctgatca ccatgatttc ttcgccaaac ggtgagcagc gtctgtttgt
     8041 gccgcgcagt ggtttcccgg atttgctggt ggatactttg ctggcgacag aagaccgtca
     8101 tttctacgag catgatggga tcagtctcta ctcaatcgga cgtgcggtgc tggcaaacct
     8161 gactgccgga cgcacggtgc agggggcgag tacgctgacg caacagttgg ttaaaaacct
     8221 gttcctctcc agcgagcgtt cttactggcg taaagcgaac gaagcctaca tggcgctgat
     8281 catggacgcg cgttacagca aagaccgtat tcttgagctg tatatgaacg aggtgtatct
     8341 cggtcagagc ggcgacaacg aaattcgcgg cttcccgctg gcgagcttgt attactttgg
     8401 tcgcccggta gaagaattga gcctcgatca gcaggcgctg ttggtgggta tggtgaaagg
     8461 ggcgtccatc tacaacccgt ggcgtaaccc gaaactggcg ctggagcgac gtaacctggt
     8521 actacgtttg ctgcaacagc aacaaattat tgatcaagaa ctctacgaca tgctgagtgc
     8581 gcgtccgctg ggtgttcagc cgcgcggtgg ggtgatctct cctcagccag cctttatgca
     8641 gctggtgcgt caggagctgc aggcgaaatt aggcgataag gtaaaagatc tctccggcgt
     8701 gaagatcttc actacctttg actcggtggc ccaggacgcg gcagaaaaag ccgccgtgga
     8761 aggcattccg gcactgaaga aacagcgtaa gttgagcgat cttgaaaccg cgattgtggt
     8821 tgttgaccgc tttagcggtg aagttcgtgc gatggtcggt ggttctgagc cgcagtttgc
     8881 gggctacaac cgtgcgatgc aggcgcgtcg ttcgattggt tcccttgcaa aaccagcgac
     8941 ttatctaacg gccttaagcc agccgaaaat ctatcgtttg aatacgtgga ttgccgatgc
     9001 gccaatcgcg ctgcgtcagc cgaatggcca ggtctggtca ccgcagaacg atgatcgtcg
     9061 ttatagcgaa agtggcaggg tgatgctggt agatgcgttg acccgttcga tgaacgtgcc
     9121 gacggtgaat ctgggcatgg cgttggggct gcctgcggtt acggagacct ggattaaact
     9181 gggtgtgccg aaagatcaac tgaatccagt tccagcaatg ttgttggggg cgttgaactt
     9241 aacgccaatc gaagtggcgc aggccttcca gactatcgcc agcggtggta atcgtgctcc
     9301 gctttccgcg ctacgttcag tcatcgcaga agacggcaag gtgttgtatc agagcttccc
     9361 gcaggcggaa cgcgctgttc cggcgcaggc ggcgtatctg acactatgga ccatgcagca
     9421 ggttgtacaa cgcggtacgg gtcgtcagct tggggcgaaa tacccgaacc tgcatctggc
     9481 agggaaaaca gggactacca acaataacgt agatacctgg tttgcgggca ttgatgggag
     9541 cacggtgacc atcacctggg tcggccgtga taacaaccag ccgaccaaac tgtatggtgc
     9601 cagcggggca atgtcgattt atcagcgtta tctggctaac cagacgccaa cgccgctgaa
     9661 tcttgttccg ccagaagata ttgcagatat gggcgtggac tacgacggca actttgtttg
     9721 cagcggtggc atgcgtgtct tgccggtctg gaccagcgat ccgcaatcgc tgtgccagca
     9781 gagtgagatg cagcagcaac cgtcaggcaa tccgtttgat cagtcttctc agccgcagca
     9841 acagccgcaa cagcaacctg ctcagcaaga gcagaaagac agcgacggtg tagccggttg
     9901 gattaaagat atgtttggga gtaattaacg tctaagcgtg atataccgga tggcgagctg
     9961 ccatccggta aaataacatc ccctccaaag tattaaccct ttcttttcat ctgattgttt
    10021 attgaccctt caggaatgcg cagattgcgt accgcttgcg aacccgccag cgtttcgaat
    10081 attatctcat ctttataata atcattctcg tttacgttat cattcacttt acaccagaga
    10141 aataccaatg gcaccttcca aaactgctca gccaaaacac tcactgcgta aaatcgcagt
    10201 tgtagtagcc acagcggtta gcggcatgtc tgtttatgca caggcagcgg ttgaaccgaa
    10261 agaagacact atcaccgtta ccgctgcacc tgcgccgcaa gaaagcgcat gggggccggc
    10321 tgcaactatt gcggcgcgac agtcagctac cggcactaaa accgatacgc cgattcaaaa
    10381 agtacctcaa tctatttctg tcgtcaccgc tgaagagatg gcgctgcatc agcctaagtc
    10441 ggtgaaagaa gcgcttagct atacgccggg tgtctctgtt ggtacgcgtg gcgcatccaa
    10501 cacctatgac cacctgatca ttcgcggttt tgcggcagaa ggccaaagcc agaataacta
    10561 tctgaatggc ctgaagttgc agggcaactt ctataacgat gcggtcatcg acccgtatat
    10621 gctggaacgt gctgaaatta tgcgtggccc ggtttccgtg ctttacggta aaagcagtcc
    10681 tggcggtctg ttgaatatgg tcagcaagcg tccgaccacc gaaccgctga aagaagttca
    10741 gtttaaagcc ggtactgaca gcctgttcca gactggtttt gactttagcg atgcgctgga
    10801 tgatgacggc gtttattctt atcgcctgac cggtcttgcg tgttctgcca atgcccagca
    10861 gaaagggtca gaagagcagc gttatgctat tgcaccggcg ttcacctagc gtccggatga
    10921 taaaaccaat ttcaccttcc tttcttactt ccagaacgag ccggaaactg gttattacgg
    10981 ctggttgccg aaagagggaa ccgttgagcc gctgccgaac ggtaagcgtc tgccgacaga
    11041 ctttaacgaa ggggcgaaga acaacaccta ttctcgtaac gagaagatgg tcggctacag
    11101 cttcgatcac gaatttaacg atacctttac tgtgcgtcag aacctgcgct ttgctgaaaa
    11161 caaaacctcg caaaacagcg tttatggtta cggcgtctgc tccgatccgg cgaatgctta
    11221 cagcaaacag tgtgcggcat tagcgccagc ggataaaggc cattatctgg cacgtaaata
    11281 cgtcgttgat gatgagaagc tgcaaaactt ctccgttgat acccagttgc agagcaagtt
    11341 tgccactggc gatatcgacc acactctgct gaccggtgtc gactttatgc gtatgcgtaa
    11401 tgacatcaac gcctggtttg gttacgacga ctctgtgcca ctgctcgatc tgtacaatcc
    11461 ggtaaatacc gatttcgact tcaatgcgaa agacccggat aactccggtc cgtaccgcat
    11521 tctgaataag cagaagcaaa cgggcgttta tgttcaggat caggcgcagt gggataaagt
    11581 gctggtcacc ctgggcggtc gttacgactg ggcagatcaa gaatctctta accgcgtagc
    11641 gggaacgacc gataaacgtg atgacaaaca gtttacctgg cgtggtggtg ttaactacct
    11701 gtttgataat ggtgtaacac catacttcag ctatagcgaa tcgtttgaac cttcttcgca
    11761 agttgggaag gatggtaata ttttcgcacc gtctaaaggt aagcagtatg aagtcggcgt
    11821 gaaatatgta ccggaagatc gtccgattgt agttactggt gccgtgtata atcttactaa
    11881 aaccaacaac ctgatggcgg accctgaggg ttccttcttc tcggttgaag gtggcgagat
    11941 ccgcgctcgt ggcgtagaaa ttgaagcgaa agcggcgctg tcggcgagtg ttaacgtagt
    12001 cggttcttat acttacactg atgcggaata cactaccgat actacctaca aaggcaatac
    12061 gcctgcacag gtgccaaaac acatggcttc gctgtgggct gactatacct tctttgacgg
    12121 tccgctttca ggtctgacgc tgggcaccgg tggtcgttat actggctcca gctatggtga
    12181 tccggctaac tcctttaaag tgggaagtta tacggtcgtg gatgcgttag tgcgttatga
    12241 tctggcgcga gtcggcatgg cgggctccaa cgtggcgctg catgtcaaca acctgtttga
    12301 tcgtgaatac gtcgccagct gctttaacac ttatggttgc ttctggggcg cagaacgtca
    12361 ggtcgttgca accgcaacct tccgtttcta atttctcttt tggggcacgg atttccgtgc
    12421 ccgtttcaca agttggctgt tatgcaggaa tacacgaatc attccgatac cacttttgca
    12481 ctgcgtaata tctcctttcg tgtgcccggg cgcacgcttt tgcatccgtt gtcgttaacc
    12541 tttcctgccg ggaaagtgac cggtctgatt ggtcacaacg gttctggtaa atccactctg
    12601 ctcaaaatgc ttggtcgtca ccagccaccg tcggaagggg agattcttct tgatgcccaa
    12661 ccgctggaaa gctggagcag caaagcgttt gcccgcaaag tggcttattt accgcagcag
    12721 cttcctccgg cagaagggat gaccgtgcgt gaactggtgg cgattggtcg ttacccgtgg
    12781 catggcgcac tagggcgctt tggtgctgca gagcgcgaaa aggtcgagga agctatctcg
    12841 ctggttggct taaaaccgct ggcgcatcgg ctggtcgata gcctctctgg cggcgaacgt
    12901 caacgggcgt ggatcgctat gctggtggcg caggatagcc gctgtctgtt gctcgacgaa
    12961 ccgacctcgg cgctggatat cgcccaccag gttgatgtac tggcgctggt gcatcgttta
    13021 agtcaggagc gaggcctgac ggtcattgcc gtgctgcacg atatcaatat ggcggcgcgc
    13081 tactgtgatt atctggtcgc cctgcgcggc ggtgaaatga ttgctcaggg aacgcctgcg
    13141 gaaattatgc gcggcgaaac cctcgaaatg atttatggca tcccgatggg tattctgccg
    13201 catccggcgg gtgctgcacc tgtgagtttt gtttattgat gagcggctta cctcttattt
    13261 cgcgccgccg actgttaacg gcgatggcgc tttctccgtt gttatggcag atgaataccg
    13321 cccacgcggc agttattgat cccaatcgta ttgtggcact ggagtggttg ccggtggagt
    13381 tactgctggc gctcggcatc gtgccttacg gcgtggcgga caccatcaac tatcgcctgt
    13441 gggtcagcga accgccattg ccggactcag tgattgacgt tggtttgcgt acagaaccca
    13501 accttgaact gctgaccgaa atgaaaccgt cgtttatggt ctggtctgca ggatatggcc
    13561 cttcaccaga aatgctggca cgtattgcgc cggggcgcgg atttaacttc agcgacggca
    13621 aacagccact ggcgatggcg cgtaaatcac tgacggaaat ggcagattta cttaacctgc
    13681 aaagcgcagc ggaaacgcat ttagcgcact atgaagactt tatccgcagc atgaaacccc
    13741 gctttgtgaa gcgtggtgca cgcccgttat tgctgacgac gcttatcgat ccgcgccata
    13801 tgttagtctt cggtccaaac agcttgttcc aggaaattct cgatgagtac ggcatcccaa
    13861 atgcctggca aggggaaacc aacttctggg gcagtaccgc cgtcagtatc gatcgtctgg
    13921 cagtgtataa agacgttgat gtgctctgtt ttgatcacga caacagcaaa gacatggatg
    13981 cgctaatggc aaccccgctg tggcaggcca tgccgtttgt ccgcgccgga cgctttcagc
    14041 gcgttccggc ggtctggttc tatggtgcga cgctctcggc aatgcacttt gtgcgtattc
    14101 tggataacgc catcggaggt aaagcgtgag taaacgaatt gcgctttttc cggtgttatt
    14161 gctggcgctg ctggtggttg ctgctgcggc gttgacctgg atgaacttct cgcaggcgct
    14221 gccgcgcagc cagtgggcgc aggccgcctg gtcgccggat attgacgtca ttgagcagat
    14281 gatttttcac tacagcttgt tgccgcgtct ggcgatttcg ctgctggtgg gcgcgggcct
    14341 ggggctggtg ggcgtgctgt ttcagcaagt gctgcgtaac ccgctggcgg aaccgacgac
    14401 gttgggtgtt gcaacaggcg cgcaactggg gattaccgtc accacgctct gggcgatccc
    14461 cggcgcgatg gcgagccagt ttgctgcgct ggcaggcgcg tgtgttgttg gcttaatcgt
    14521 ctttggcgtc gcgtggggga aacggctttc gccggtaacg ctgatcctcg cggggctggt
    14581 agtgagcctt tattgcggcg caatcaatca gttactggtt atcttccatc atgaccaact
    14641 gcaaagcatg ttcctgtgga gcaccggaac gctgacgcaa accgactggg gcggcgttga
    14701 gcgtttatgg ccgcagctgc tgggcggcgt gatgctgacg ttattgctac ttcgcccgtt
    14761 aactctgatg gggcttgatg atggcgtggc gcgcaatctc gggctggcct tgtcgctcgc
    14821 tcgtctggcg gcgttgtcgc tggcgattgt catcagtgcg ctgctggtga acgcggtggg
    14881 gattatcggc tttatcggtt tgttcgcgcc actgctggtg aaaatgctgg gggcgcggcg
    14941 tttgttgcca cggttgatgc tggcatcgct gattggtgcg ttaattctgt ggctttctga
    15001 tcaaatcatc ctctggctga ctcgcgtgtg gatggaagtg tccaccggtt cggtcactgc
    15061 gttgatcggt gcgccgctgc tactgtggtt gctgccgcgt ttacgcagca ttagcgcgcc
    15121 ggatatgaag gtgaacgatc gtgtcgcgac tgaacgccaa cacgtgctgg cgtttgccct
    15181 cgcgggcggc gtgctgctgt tgatggctgt ggtggtggcg ctgtcgtttg gtcgtgatgc
    15241 gcacggctgg acgtgggcga gcggggcgtt gctcgaggat ttgatgccct ggcgctggcc
    15301 gcgaattatg gctgcgctgt ttgcgggcgt catgctggcg gtggcgggct gtattattca
    15361 gcggctgacc ggaaacccga tggcaagccc ggaagtgctg gggattagct ccggcgcggc
    15421 gtttggcgtg gtgttaatgc tgtttctggt gccgggtaat gcctttggct ggctgttacc
    15481 cgcaggcagt ctcggggcgg cggtgacgct gttgatcatt atgatcgccg ccggacgtgg
    15541 tggattttcc ccacaccgca tgttactggc ggggatggcg ttaagtaccg cgttcaccat
    15601 gcttttgatg atgttgcagg caagtggtga cccgcgaatg gcgcaagttc tgacctggat
    15661 ttccggttcg acctacaacg cgaccgatgc gcaggtctgg cgtaccggaa ttgtgatggt
    15721 gattttgctg gcgattaccc cgctgtgccg ccgctggctg accattttac cgctgggtgg
    15781 tgataccgca cgagccgtgg ggatggcgct gacgccgacg cgaattgcgc tgctgctgtt
    15841 agcggcttgc ctgacggcga ccgcgacaat gaccattgga ccgttgagtt ttgttggttt
    15901 aatggcaccg catattgcgc ggatgatggg ctttcgacgg acgatgccac acatcgtaat
    15961 ttcggcgctg gtgggtggtt tactgctggt gttcgctgac tggtgtgggc ggatggtgct
    16021 gtttccattc cagatccccg cggggctgct gtcgaccttt atcggcgcgc catattttat
    16081 ctatttgttg agaaagcaga gccgttaata gaatgcctcg cccggtgggc gaggcaaaat
    16141 cattacaact tcgcaaacac ccgatgtgca gcatcgatgg tgttattgat atcttccatg
    16201 ctatgcgcca cggacataaa gcccgcttca aacgctgacg gtgccaggta aacaccttcg
    16261 tccagcatca tatggaagaa acgcttaaag cgttccacgt cacaggccat cacatcctga
    16321 tagcacgtca cggactcggc gtcggtaaag aaaataccga acatgccgcc aacgtggttt
    16381 accaccagcg gaattccggc ttcttctgcc gcttcccgca gaccttctgc cagacgtgtt
    16441 gtcagctcat ccagcgtttc gtgaacgccc ggctgcgcga cttcattcag acaggcgaaa
    16501 ccggctgcca tcgcaatcgg gttaccggaa agcgtacccg cctgatatac cggaccggtc
    16561 ggggccagcg catccattac atcacgacga ccaccgaatg cgcctaccgg cattccaccg
    16621 ccgatgattt tgccgaggca ggtgagatcc ggttccacac cgtaataatc ctgtgcgcca
    16681 gctagcgcta cgcggaaacc ggtcatcact tcatcgatga tcagcaacgc gccaaattcg
    16741 tcgcacagcg cgcgcagacc tggcaggaac tctggcagcg gtggaacgca gttcatattg
    16801 cctgccaccg gctcgacgat aatacaggca atctcttgcg ggtattgctc aaacgcggcg
    16861 cgtacagagg ccagatcgtt ataagtacag gttaaggtat gtttggcgaa atctgccgga
    16921 acgcccggcg aatttggctg gcctaacgtg agtgcgccag aaccggcttt caccagcagg
    16981 cagtcagcgt gaccgtggta acaaccttca aatttaataa ttttgtcgcg accggtaaaa
    17041 ccacgggcca ggcggatggc gctcatcgtc gcttcagtgc cggagttcac catgcgcacc
    17101 atatccatgg tcgggaccag ttcggtcacc agttgcgcca ttttcacttc catttcggtt
    17161 ggtgcaccaa agcttaaacc acgctcggcg gcttcaatca cggcattgcg gatcgccgga
    17221 tggttatggc ccagcaccat cggcccccag gaaccgacat aatcgatata ggctttgcca
    17281 tcaacatcgt acagataagc gccgtccgct ttttcgataa acagtggagt gccgcccacg
    17341 ccagtaaagg cgcgaacagg ggagttcaca ccgccaggga tcagctcgcg cgctgcgctg
    17401 taaagatttt cagacttact catggagggt tcctgattcg tagaaaaagt gaatggctgc
    17461 tattctatgt tattcataac aagttaaata ctcgtcaaac atcaggctgc ttgtactggt
    17521 gcaatcctga tttcttagag tataaaagtt ttgtgcattt gaaacattac gctttgcaaa
    17581 ggattttcat ggaacgtgcg agtaaaatgc cgtcatctta tttgtatgac caataagtga
    17641 tcattggatg aaaactgata ctccctcttt agaaacaccg caggccgcgc gcctgcgacg
    17701 cagacaactg attcgccaac ttcttgagcg cgataaaacc ccgttagcca ttttgtttat
    17761 ggcggcagtc gtcggcacgc ttgtcgggct ggctgcggtt gcttttgaca aaggtgtcgc
    17821 ctggttgcag aaccaacgta tgggggcact ggtacatact gctgataatt atccgctact
    17881 gttaaccgtc gcttttctct gttcggcggt gctggcgatg ttcggctact ttttggtgcg
    17941 caaatacgcg cccgaagcag gggggtcggg gatcccggaa attgaagggg cgctggaaga
    18001 tcaacgtccc gttcgctggt ggcgtgtgtt gccggtgaaa ttctttggcg ggctggggac
    18061 actcggcgga ggcatggtgc tggggcgcga agggccaacc gtgcagatcg gcggtaatat
    18121 cgggcggatg gtactcgatg ttttccgcct gaaaggtgac gaagctcgtc atacgctact
    18181 ggcaaccggt gctgcggcgg ggctggctgc ggcctttaac gcgccgctgg cgggtatttt
    18241 gtttattatc gaagagatgc gtccgcagtt tcgctatacg ttaatttcga ttaaagcggt
    18301 atttattggc gtcattatgt cgaccattat gtaccggatt tttaatcatg aagttgcgtt
    18361 gattgacgtc ggtaaacttt ctgacgcgcc gcttaatacg ctgtggcttt atctgatcct
    18421 cggtattatt tttggcattt tcggccctat ttttaataaa tgggtgctgg gaatgcagga
    18481 tttgctgcac cgtgtgcacg gcggcaatat taccaaatgg gtagtgatgg gcggtgcgat
    18541 tggcggtctg tgtggattgc tggggtttgt ggcaccagca acgtcgggcg gcggctttaa
    18601 cctgattcct atcgctaccg cagggaattt cagcatggga atgctggtgt ttatcttcgt
    18661 cgcgcgggtc attaccactt tactctgctt ctcttccggc gcgccgggcg gtatttttgc
    18721 cccgatgctg gcgctgggta ctgtgctggg aaccgctttc ggaatggttg ccgttgagtt
    18781 gtttccgcaa tatcaccttg aggcggggac gtttgctatt gccggaatgg gggcgttact
    18841 ggcggcatct attcgcgcgc cgttaacggg gatcattctg gttctggaaa tgaccgataa
    18901 ctaccagctc attttgccaa tgattattac cggtcttggc gcaacactat tagcacaatt
    18961 taccggtggg aaaccgctat actcggcgat tcttgcgcgt acgctggcaa aacaggaagc
    19021 tgagcaactg gcgcgaagca aggccgcatc agcccgcgag aatacttgaa cgaaatacca
    19081 gggtattaga taatggcgat tattattggg ttagaatttg cccaattgcc gatgtcgttt
    19141 ggagcaaaat atgagtgatg acgtagcact gccgctggag tttaccgacg cagcagccaa
    19201 caaagttaaa agcctgatcg ctgacgaaga taacccgaat ctgaaattac gcgtgtatat
    19261 caccggtggc ggttgcagcg gcttccagta tggtttcacc tttgatgatc aggtgaacga
    19321 aggcgatatg accatcgaaa aacagggcgt tggcctggtg gttgatccga tgagcctgca
    19381 atatctggtc ggcggttccg ttgactatac cgaaggtctg gaaggttctc gtttcatcgt
    19441 gaccaacccg aacgcgaaaa gcacctgcgg ttgcggttct tccttcagca tataatctgt
    19501 tgttcatgtg ccggataaac tatccggcac atcttctctc ttcaacgccc attctcatcc
    19561 agcgcaaacg tcggtagctt aagatgccaa cgaatagccg ccagccgaat caatagcgtc
    19621 acgaccatgc ccatcatact ggctgtttcc agtggtacgg aaaatgtgta ataagccgta
    19681 gcgtggacaa taccgccgat aatacaggca gttgcgtaga tttctgtacg taaaatcatg
    19741 gggatttcgc gggccagtac atcacgaatg atcccgccgc caacgccagt aatgacgccc
    19801 atacaaaccg cgattaacgg accggcttcc gcattaaagg ctttattcac gccaatgccg
    19861 acaaacaccg ccagaccaac ggcgtccagc accggcaaca tccattttgg taagcgtctt
    19921 ggctggcgca ccagcacgat ggtcagcatg ctggtgacca ttgcaacgac cagatcggtg
    19981 ggatctttca cccaaaatac cgggccgtga tccagcgcca tgtcgcgaat tgttccgccg
    20041 cctactgcgg taaccacgcc caataccaga acgccaaaag ggtccatacg caattttccg
    20101 gctaacaaaa cgccagagat agcaaatacc gctgtgccga ctatatccag ccaatagacg
    20161 agcattgttc aatccccact gagcacctga aaaggtcagg cgctaatcta cctgtgaaag
    20221 cgcattacag agctgttgtg cagcgaggat aatacgtggg cttgcacgtt caaaccagtc
    20281 actcgtgaga ggaataacgg gaattttgag ctgttcaccc cagtattgtt tgattttagg
    20341 aatttggtcc ggtccgcctg taatgacaat cgcctgtggc gagcgtgcta acacctgttc
    20401 gcggctaact tgcggccagg gaacccggct gtctttaaag atgttttctc cgccacaaac
    20461 ttcgagtacc tggttctgaa tcgactcttt tccactggta aatggcggat taatgccgaa
    20521 ttgcagaaaa acacgttttt taggtttatc agcatattgc gctttcaatt gcgcgtactg
    20581 atccagcagg gattgcgcgg cttgttcggc cttatccggt tgcggactcc agggggccag
    20641 ttgacgtaac acattggcaa tttgttcaat gcttgtcgca tcgacccaca tcacttttat
    20701 tcccagcgaa gccagctggt caacctgccg ctcggcatta cctccacgcc aggcaatcac
    20761 cagatcgggt ttcagcgcga caatgcgttc cagattcatc ccctgccagg tggaaacctg
    20821 ctcaatcttt tgcgcttgtg gaggatagtc ggaatagctg ctgaccccaa ccggcgtgat
    20881 cccggcggca aaggcaagtt cagtgttggc gggagaaagc gtgatgacgc gcggcgcggc
    20941 attgagccac agcggcgcga gaaaagacag ggcgaccagc gccctgaaca gtgacttagc
    21001 catgtgccag tttctgcacc agtgactcaa ccatcaggct ggactgttta gcggcaacag
    21061 ccaggaactc atcgaagcta agatgagact gttgatcggc cacgtcggaa atggcgcgta
    21121 ctacgacaaa cgggacgttg aaattgtggc agacatgggc gattgccgtc gcttccatct
    21181 ctacagcaat ggcctgtggg aagttgtggc ggattttcgc cagaccaaca gaaccgttga
    21241 tgaaagcgtc gccgctaaca atcaggccac gtacagcgtt aagattcagt tcggcaatgc
    21301 aggcctcagc ggcagcgatc agtttatcgt cagctttgaa gcctgccggg cagcctggta
    21361 actgaccgta ttcataacca aatgccgtga catccgcgtc gtgataacgc gcttcgtccg
    21421 agacaacgat atcgcccact ttcagcgttg gtgccaggcc accggcagaa ccggtgttaa
    21481 taatcacatc tggcttgcag tgttccaaca gcaaagtggc acccagcgct gcagcgactt
    21541 taccgatgcc cgatttcaga agcgcaacct cggttccatt cagttggccg gtatagattt
    21601 cgcaaccgcc cagactgata gtttgacggt tttcgatttt gtcacgcagc agcgtaactt
    21661 cttcttccat tgcaccaatg atgccgattt tcatagattt actcgcgata agcccgattt
    21721 gaaggcatag tttaccatgc gcttacgggg aagcgtattt ctcacgcggg agaggacatg
    21781 acacagattg atttccgaaa aaaaataaac tggcatcgtc gttaccgttc accgcagggc
    21841 gttaaaaccg aacatgagat tctgcggatc ttcgagagcg atcgcgggcg tatcatcaac
    21901 tctccggcaa ttcgtcgtct gcaacaaaag acccaggttt ttccactgga gcgcaatgcc
    21961 gccgtgcgca cgcgtcttac ccactcgatg gaagtccagc aggtggggcg ctacatcgcc
    22021 aaagaaattt taagccgtct gaaagagctt aaattactgg aggcgtatgg tctcgatgaa
    22081 ctgaccgggc cctttgaaag cattgttgag atgtcatgcc tgatgcacga tatcggcaat
    22141 ccgccgtttg gtcattttgg cgaagcggcg ataaatgact ggtttcgcca gcgtttgtac
    22201 ccggaagatg tcgaaagcca gcctctgact gacgatcgct gcagcgtggc ggcactacgt
    22261 ttacgggacg gggaagaacc gcttaacgcg ctgcggcgca agattcgtca ggacttatgt
    22321 cattttgaag gtaacgcaca gggtattcgt ttggtgcata cattgatgcg gatgaatctc
    22381 acctgggcac aggttggcgg tattttaaaa tatacccgtc cggcgtggtg gcgtggtgaa
    22441 acgcctgaga cacatcacta tttaatgaaa aagccgggct attatctttc cgaagaagcc
    22501 tatattgccc ggttgcgtaa agaacttaat ttggcgcttt acagtcgttt tccgttaacg
    22561 tggattatgg aagctgccga cgacatctcc tattgtgtgg cagaccttga agatgcggta
    22621 gagaaaagga tatttaccgt tgagcagctt tatcatcatt tgcacgaggc gtggggccag
    22681 catgagaaag gttcgctctt ttcgctggtg gttgaaaatg cctgggaaaa atcacgctca
    22741 aatagtttaa gccgcagtac ggaagatcag ttttttatgt atttacgggt aaacacccta
    22801 aataaactgg taccctacgc ggcacaacga tttattgata atctgcctgc gattttcgcc
    22861 ggaacgttta atcatgcatt attggaagat gccagcgaat gcagcgatct tcttaagcta
    22921 tataaaaatg tcgctgtaaa acatgtgttt agccatccag atgtcgagca gcttgaattg
    22981 cagggctatc gggtcattag cggattatta gagatttatc gtcctttatt aaacctgccg
    23041 ttatcagact ttactgaact ggtagaaaaa gaacgggtga aacgtttccc tattgaaacg
    23101 cgcttattcc acaaactctc gacgcgccat cggctggcct atgtcgaggc tgtcagtaaa
    23161 ttaccgtcag attctcctga gtttccgcta tgggaatatt attaccgttg ccgcctgctg
    23221 caggattata tcagcggtat gaccgacctc tatgcgtggg atgaataccg acgtctgatg
    23281 gccgtagaac aataaccagg cttttgtaaa gacgaacaat aaatttttac cttttgcaga
    23341 aactttagtt cggaacttcg cgctataaaa cgaatctgaa gaacacagca attttgcgtt
    23401 atctgttaat cgagactgaa atacatgaaa aaaaccacat tagcactgag tgcactggct
    23461 ctgagtttag gtttggcgtt atctccgctc tctgcaacgg cggctgagac ttcttcagca
    23521 acgacagccc agcagatgcc aagccttgca ccgatgctcg aaaaggtgat gccttcagtg
    23581 gtcagcatta acgtagaagg tagcacaacc gttaatacgc cgcgtatgcc gcgtaatttc
    23641 cagcagttct tcggtgatga ttctccgttc tgtcaggagg gttctccgtt ccagagctct
    23701 ccgttctgcc aggggggcct gggcggtaat ggcggcggcc agcaacagaa attcatggcg
    23761 ctgggttccg gcgttatcat tgatgccgat aaaggctatg tcgtcaccaa caaccacgtt
    23821 gttgataacg cgacggtgat taaagtccaa ctgagcgatg gtcgtaagtt cgacgcgaag
    23881 atggttggca aagatccgcg ctctgatatc gcgctgatcc agatccagaa cccgaaaaac
    23941 ctgaccgcaa ttaagatggc ggattctgat gcgctgcgcg tgggtgatta caccgtagca
    24001 attggtaacc cgttcggcct gggcgagaca gtaacttccg ggattgtctc tgcgctggga
    24061 cgtagcggcc tgaacgctga aaactacgaa aacttcatcc agactgatgc agcgatcaac
    24121 cggggtaact caggtggtgc gttggttaac ctgaacggcg aactgattgg tatcaacacc
    24181 gcgattctcg caccggacgg cggaaacatc ggtatcggtt ttgctatccc gagcaacatg
    24241 gtgaaaaacc tgacctcgca gatggtggaa tacggccagg tgaaacgtgg tgagttgggt
    24301 attatgggca ctgagctgaa ctccgatctg gcgaaagcga tgaaagttga cgctcagcgc
    24361 ggtgctttcg taagccaggt tctgccgaat tcttccgccg cgaaagcggg cattaaagcg
    24421 ggtgatgtga tcacctcact gaacggtaag ccaatcagca gctttgccgc actgcgtgct
    24481 caggtgggca ctatgccggt aggtagcaaa ctgaccctgg gcttactgcg cgacgggaag
    24541 caggttaacg tgaacctgga acttcagcag agcagccaga atcaggttga ttccagcacc
    24601 atcttcaacg gcattgaagg cgctgagatg agcaacaaag gcaaagatca gggcgtggtg
    24661 gtgaacaacg tgaaaacggg cactccggct gcgcagatcg gcctgaagaa aggtgatgtg
    24721 attattggcg cgaaccagca ggcagtgaaa aacatcgctg aactgcgtaa agttctcgac
    24781 agcaaaccgt ctgtgctggc actgaacatt cagcgcggcg acagcaccat ctacctgtta
    24841 atgcagtaat ctccctcaac cccttcctga aaacgggaag gggttctcct tataatctgt
    24901 gaacttcacc acaacttcat acatcttcat catcctttgg gcatttgcac aatgccgtac
    24961 gttacgtact tccttatgct aagccgtgca taatggagga cttatggctg gctggcatct
    25021 tgataccaaa atggcgcagg atatcgtggc acgtaccatg cgcatcatcg ataccaatat
    25081 caacgtaatg gatgcccgcg ggcgaattat cggcagcggc gatcgtgagc gtattggtga
    25141 attgcacgaa ggagcattgc tggtactttc acaaggacga gtcgtcgata tcgatgacgc
    25201 ggtggcacgt catctgcacg gtgtgcggca ggggattaat ctaccgttac ggctggaagg
    25261 tgaaattgtc ggcgtaattg gcctgacagg tgaaccagag aatctgcgta aatatggcga
    25321 actggtctgc atgacggctg aaatgatgct ggaacagtcg cggttgatgc acctgttggc
    25381 gcaggatagc cgtttgcggg aagaactggt gatgaacctg attcaggctg aggagaatac
    25441 tcccgcactt actgaatggg cgcaacggct ggggatcgat ctcaatcaac cgcgagtggt
    25501 ggctattgtt gaggtcgaca gcggtcagct tggtgtggac agcgcaatgg cggagttaca
    25561 gcaactgcaa aatgcgctga ctacgcccga gcgtaataat ctggtggcga ttgtctcgct
    25621 aaccgaaatg gtggtgttga aaccggcgtt gaactctttt gggcgctggg acgcagaaga
    25681 tcatcgtaag cgagttgaac aactgattac ccgcatgaaa gagtatggcc agttgcgttt
    25741 tcgcgtttcg ctgggcaact attttaccgg tcctggcagt attgcccgat cctatcgtac
    25801 ggcgaaaacg acgatggtgg tggggaaaca gcggatgcca gaaagtcgct gctattttta
    25861 tcaggatctg atgttacctg tgttactcga cagtttgcgt ggcgactggc aggccaacga
    25921 actggcgcga ccgctggcgc ggctgaaagc gatggacaat aacggcttgc tgcggcgaac
    25981 gctggcggcg tggtttcgcc acaatgtgca accgctggca acgtcaaagg cgttgtttat
    26041 tcatcgtaat actctggaat atcggcttaa tcgtatatcg gaactgaccg ggcttgattt
    26101 gggcaatttt gatgacaggt tgctgctgta tgtggcgtta caactggatg aagagcggta
    26161 ggtcgattac aaaattacag ccgggtgaga ctttacccgg ccacagaatg gcattattta
    26221 cgcgtcagtt tttccagatc ggcttcaatc tcgctgatct tattggttac tactgactcc
    26281 aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg
    26341 cagatttgat caagctcatc aatgatatac cgtagattcg ggctaatttc ctggacttct
    26401 ttataaccct gacccacacc atcagcgacg accgttttac gctgacgcgg gtatttaaac
    26461 ttcacgctct tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg
    26521 ttgttggctt cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga
    26581 cttttcagat tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt
    26641 agcatttggc ctggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac
    26701 ttctatcatg gcgtaaaaat caaaaaatta cctgctttat tgtggtgata ttctatggga
    26761 tggattagca catttatctt cattttgcga attacatgac agtccgaaac aagatgaaaa
    26821 agtatttaaa aaaaaggggg aggaggaaga atgagtagga cgaattattc tgagccagaa
    26881 agcaatcggg cagcgtcgtg ctgcccgtgt acatactttt agtcgatggt acgcagcagt
    26941 tcgttaatac caactttgcc gcgagttttc gcgtcaactt tcttaacgat aactgcacag
    27001 tagaggctgt atttgccatc tttcgacggc agattacctg aaacaaccac agaccctgcc
    27061 ggaacgcgac cgtagtggat ttcgccggtt tcacggtcat aaatacgggt gctctgacca
    27121 atgtatacgc ccatagagat gacggaacct tcttcgacaa tcaccccttc aaccacttca
    27181 gagcgtgcgc cgatgaagca gttatcttca ataatggtcg ggttagcctg cagcggctcc
    27241 agcacgccgc cgatacccac gccaccggaa aggtggacgt ttttaccaat ctgcgcacag
    27301 gaaccgacgg tcgcccaggt atcaaccatg gtgccttcat caacatatgc gccgatgttg
    27361 acgtaagacg gcatcagcac ggtgttacgg gcaatgaacg cgccctgacg cactgccgct
    27421 ggtggcacga cgcggaagcc ttctttctgg aaacgtgctt catcgtagtt agcgaatttc
    27481 atcggcactt tgtcgaagta gcggctttct gccccttcga tcacctgatt atcattaata
    27541 cggaaagaga gcagcaccgc ttttttcaac cactgatgcg tcacccactg accgtcaatt
    27601 ttttccgcta cacgcagtgc gccggaatcc agcagggcga tcacctgatt taccgcttcg
    27661 cgggtaacgg tgtctgcatt ggctggcgtg atctcggcac ggcgttcaaa agcggtttca
    27721 ataatgttct gtaactgctg cattgttaaa ctcttttcat atcagtaaac acatcaccct
    27781 ttatcgtttg gattgagggc ctctgtcaac cgctgatgca cttcctgctg caactcgtta
    27841 ttaagcgcac gccggtcagc ggtggcaatt atgaataaat cttctactcg ctcgccaatg
    27901 gttgtaattc gggcgccatg aagcgaaatt cccagatcgg caaaaatttt cccgactcgc
    27961 gccagcagtc caggttggtc gagggcgatc agttcgagga acgatttgcg gtcggtatgg
    28021 gtcggcaaaa acgtcacttc ggtttcaaca gtaaaatggc gcaatttggc aggttggcga
    28081 cgcggctgcg gtggctgcca gctactttgt gtcagcacct gctccagacc aaaccgaata
    28141 acctcatgac gatcggcgga cagcgggctg ccatcgggtt ccagtacgat gaaggtatcc
    28201 atcgccatac cgtcacgagt ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg
    28261 tctaattcgg cacagacggc ggcaaacaga taggggcggt ccgggctcca gataaaaatc
    28321 tcggtgcctc cacgcgtagc ctgcgggcta agcagtacca gcggtttgct taaatcatgc
    28381 tgtaataaat ggcgtgcgtg ccaggccagt tgatttgggc tatggcggac aaaatagtta
    28441 gcgcgacagc gtgaccaaat ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt
    28501 agtgccagcg catggagttg gtgatggcga acccgttcgc gcatatccgg cgtgttttgc
    28561 attccgcgtc gtagctgctt ttcggtggca aaatagagtt cgcgcaacag actttgcttc
    28621 cagctattcc acagtgtttc gttggtggcg caaatgtcag ccactgtcag gcataccaga
    28681 tagcgcagac gattttccgt ttgaacttct tcggcaaact gcttgatgac ttccgggtcc
    28741 tgaatatcgc ggcgctgagc ggtcaccgac atcagcaagt gctggcgaac cagccaggcg
    28801 accaactgtg tttcgcgtga gttcagcccg tggagttcgg caaaatgcac tacatcctga
    28861 gcaccgagaa tggagtggtc gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg
    28921 atgaaaatca gctcagttga cggcaggcgc ggccagacgt ccacacacaa cggatggcgc
    28981 tggcgcgttt ctttactggc aaaactctcc agtttcagca tcacgcggat agtatgttca
    29041 tccaccgtgt aggcgtggaa cagatcaaac tgcatctgcc cgacgatatg cgaccattgc
    29101 ggcatatacg cgccgagtac gctatggcga tgcattggca atagcccgcg ccgcaccgct
    29161 ccggggtgac gaagaatgct caaaaacagt tttcgcgctt ccggaatatt acacagaggt
    29221 tgttgcagat ggcgacgggc gtggcgtaac tggcgtagcg tggtggagta aatgccggtg
    29281 atcgcactgt tgcgcaccat ggtgtagaac atacgcaaga tggcttccgg ctggcgcata
    29341 aatagtgttt catcacgcag gtcgatcagc gttccgcgta gctgaaactc atcgtcgatt
    29401 ggacgtggtt tttcgtcggc gggaagggcg aggatggctt catcgaacag ttgcagcagc
    29461 atctggttga gttcactgac gcggcgtgta acgcggaagt aatccttcat catccgctcg
    29521 accggttcgt taccttcacc actataattc aaacgctggg cgacgctaag ctggcgatcg
    29581 aataacaggc gattatcgta acgactgacg accagatgca gggcaaagcg aatgcgccac
    29641 aatatatgca ggcattcgtt taattccgct ctctccgcag aggttaagaa gccaaaccct
    29701 accatttcat ccagcgatgt tgctccaaaa tgacggcggg ccacccattg cagagtgtgg
    29761 atatcgcgca agccgccggg gctacttttg atgtctggtt caaggttgta gctggtgccg
    29821 tggtaacgct gatggcgctg gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa
    29881 ggccagaatc cttcgctaaa aatatgtttt tgcagttcga ggaacagcgc aacatcgcca
    29941 attaataagc gggattcgat taaattggtg gcgacggtta aatccgataa cccttccagc
    30001 atgcactctt caagcgtgcg cacgctatga cctacttcca gctttacatc ccagagcagc
    30061 gttaacagct cgcccacttt ttgcgcctga tcgtccggga gctttttacg gcttaaaatc
    30121 agtaaatcga tgtcagaaag tggatgcagc tcgccacgac cgtagccacc gacggcgacc
    30181 aatgccaggt cggcaatctg gctgaatccc gcttcaatcc ataatcgttg aagaagctgg
    30241 tcgataaact cggtacgcgc ctcaatcaac tgttctgcgg agataccatt gtcaaaggca
    30301 tcacccagcc aacgctggaa agtatcgata tgggctttta tcccaccgac ggttaattca
    30361 tcacggggcc aggcgcatgg attttgcggt tgaccgtgca gggtggggag agcggtgttt
    30421 gcgtactgtt ctggaagggt attcattgtg cgccacccat aagattaaat tattgcatta
    30481 aaaaagccgg cattcgccgg cttcatttta ttcgtcgtgc gagattatcg ccgggatggt
    30541 gtcatccttg cgtagcgtca gaatttcgca gccgttatca gtcaccacaa tagtatgctc
    30601 atattgtgca gacaagctgc gatctttggt ttttaccgtc cagccatctt tcatggtgcg
    30661 gatctctttt ttacccgcgt tgaccattgg ctcgatggtg aacgtcatcc ccggtttcag
    30721 cacgacgttg gtttcacggg agtcatagtg cagcacctgc ggttcttcgt ggaagccgcg
    30781 accaataccg tgtccacaat attcacgaac gacggagaag ccttctgctt cgacaaattt
    30841 ctgaattgcc gcaccgattt cgcgcagatt aatgccaggt tttaccatgc gtagcgccag
    30901 gtacaggctt tcttgcgtga tgcggcacag acgttcgccc atgatggtcg gcttaccgac
    30961 gataaacatt ttcgaggtat cgccgtggaa accatctttg attacggtga catcaatgtt
    31021 aacgatatcg ccatctttca gcagcttggc gtcgtccggg ataccgtggc acaccacttc
    31081 attaatagag atgcaaacgg atttcggata gccgtgatag ccgaggcagg cagaaaccgc
    31141 gtgttgttca ttaacaatgt aatcattaca gatgcgatcc agctcgccgg tgctgacgcc
    31201 cggtttaaca tacggttcga tcatctccag cacttcggca gccagtcggc cagcgacgcg
    31261 cattttttcg atatcttctg gggtcttgat tgagatagcc attaattctg tccatcagcg
    31321 tcggtgatac cgacaatata tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg
    31381 agaatcattc tgaatttcgc caaacgtgcc actgaaggtt ttctataata gaaaattcaa
    31441 cgtctgatgc tgtacacagc gccaacaatt attggtgtcc acgacgtatt tgtggtataa
    31501 agcgcgccgg acttccgatc catttcgtat acacagactg gacggaagcg acaatctcac
    31561 tttgtgtaac aacacacacg tatcggcaca tattccgggg tgccctttgg ggtcggtaat
    31621 atgggatacg tggaggcata accccaactt ttatatagag gttttaatca tggcaactgt
    31681 ttccatgcgc gacatgctca aggctggtgt tcacttcggt caccagaccc gttactggaa
    31741 cccgaaaatg aagccgttca tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga
    31801 gaaaactgta ccgatgttca acgaagctct ggctgaactg aacaagattg cttctcgcaa
    31861 aggtaaaatc cttttcgttg gtactaaacg cgctgcaagc gaagcggtga aagacgctgc
    31921 tctgagctgc gaccagttct tcgtgaacca tcgctggctg ggcggtatgc tgactaactg
    31981 gaaaaccgtt cgtcagtcca tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg
    32041 tactttcgac aagctgacca agaaagaagc gctgatgcgc actcgtgagc tggagaaact
    32101 ggaaaacagc ctgggcggta tcaaagacat gggcggtctg ccggacgctc tgtttgtaat
    32161 cgatgctgac cacgaacaca ttgctatcaa agaagcaaac aacctgggta ttccagtatt
    32221 tgctatcgtt gataccaact ctgatccgga cggtgttgac ttcgttatcc cgggtaacga
    32281 cgacgcaatc cgtgctgtga ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg
    32341 ccgttctcag gatctggctt cccaggcgga agaaagcttc gtagaagctg agtaataagg
    32401 cacgctcgta cgagccctta tagaccaggt agtacacgtt tggttagggg gcctgcatat
    32461 ggcccccttt ttcactttta tatctgtgcg gtttaatgcc gggcagatca catctccgag
    32521 gattttagaa tggctgaaat taccgcatcc ctggtaaaag agctgcgtga gcgtactggc
    32581 gcaggcatga tggattgcaa aaaagcactg actgaagcta acggcgacat cgagctggca
    32641 atcgaaaaca tgcgtaagtc cggtgctatt aaagcagcga aaaaagcagg caacgttgct
    32701 gctgacggcg tgatcaaaac caaaatcgac ggcaactacg gcatcattct ggaagttaac
    32761 tgccagactg acttcgttgc aaaagacgct ggtttccagg cgttcgcaga caaagttctg
    32821 gacgcagctg ttgctggcaa aatcactgac gttgaagtac tgaaagcaca gttcgaagaa
    32881 gaacgtgttg cgctggtagc gaaaattggt gaaaacatca acattcgccg cgttgctgcg
    32941 ctggaaggcg acgttctggg ttcttatcag cacggtgcgc gtatcggtgt tctggttgct
    33001 gctaaaggcg ctgacgaaga gctggttaaa cacatcgcta tgcacgttgc tgcaagcaag
    33061 ccagaattca tcaaaccaga agacgtatcc gctgaagtgg tagaaaaaga ataccaggta
    33121 cagctggata tcgcgatgca gtctggtaag ccgaaagaaa tcgcagagaa aatggttgaa
    33181 ggccgcatga agaaattcac cggcgaagtt tctctgaccg gtcagccgtt cgttatggaa
    33241 ccaagcaaaa ctgttggtca gctgctgaaa gagcataacg ctgaagtgac tggcttcatc
    33301 cgcttcgaag tgggtgaagg catcgagaaa gttgagactg actttgcagc agaagttgct
    33361 gcgatgtcca agcagtctta attatcgaaa aggagccgcc tgagggcggc ttctttttgt
    33421 gcccatcttg taaattcagc taacccttgt ggggctgcgc tgaaaagcga cgtacaatgt
    33481 cgctagtatt aattcatttc aatcgttgac agtctcagga aagaaacatg gctaccaatg
    33541 caaaacccgt ctataaacgc attctgctta agttgagtgg cgaagctctg cagggcactg
    33601 aaggcttcgg tattgatgca agcatactgg atcgcatggc tcaggaaatc aaagaactgg
    33661 ttgaactggg tattcaggtt ggtgtggtga ttggtggggg taacctgttc cgtggcgctg
    33721 gtctggcgaa agcgggtatg aaccgcgttg tgggcgacca catggggatg ctggcgaccg
    33781 taatgaacgg cctggcaatg cgtgatgcac tgcaccgcgc ctatgtgaac gctcgtctga
    33841 tgtccgctat tccattgaat ggcgtgtgcg acagctacag ctgggcagaa gctatcagcc
    33901 tgttgcgcaa caaccgtgtg gtgatcctct ccgccggtac aggtaacccg ttctttacca
    33961 ccgactcagc agcttgcctg cgtggtatcg aaattgaagc cgatgtggtg ctgaaagcaa
    34021 ccaaagttga cggcgtgttt actgctgatc cggcgaaaga tccaaccgca accatgtacg
    34081 agcaactgac ttacagcgaa gtgctggaaa aagagctgaa agtcatggac ctggcggcct
    34141 tcacgcttgc tcgtgaccat aaattaccga ttcgtgtttt caacatgaac aaaccgggtg
    34201 cgctgcgccg tgtggtaatg ggtgaaaaag aagggacttt aatcacgaaa taattcccgt
    34261 gatggataaa taagggtaag attccgcgta agtatcgcgg gggatcttac ccgtaatatg
    34321 tttaatcagg gctatactta gcacacttcc actgtgtgtg actgtctggt ctgactgaga
    34381 caagttttca aggattcgta acgtgattag cgatatcaga aaagatgctg aagtacgcat
    34441 ggataaatgc gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg gtcgtgcttc
    34501 tcccagcctg ctggatggca ttgtcgtgga atattacggc acgccgacgc cgctgcgtca
    34561 gctggcaagc gtaacggtag aagattcccg tactctgaaa atcaacgtgt ttgatcgttc
    34621 aatgtctccg gccgttgaaa aagcgattat ggcgtccgat cttggcctga acccgaactc
    34681 tgcgggtagc gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc gtaaagatct
    34741 gaccaaaatc gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta atgtgcgtcg
    34801 tgacgcgaac gacaaagtga aagcactgtt gaaagataaa gagatcagcg aagacgacga
    34861 tcgccgttct caggacgatg tacagaaact gactgatgct gcaatcaaga aaattgaagc
    34921 ggcgctggca gacaaagaag cagaactgat gcagttctga tttcttgaac gacaaaaacg
    34981 ccgctcagta gatccttgcg gatcggctgg cggcgttttg ctttttattc tgtctcaact
    35041 ctggatgttt catgaagcaa ctcaccattc tgggctcgac cggctcgatt ggttgcagca
    35101 cgctggacgt ggtgcgccat aatcccgaac acttccgcgt agttgcgctg gtggcaggca
    35161 aaaatgtcac tcgcatggta gaacagtgcc tggaattctc tccccgctat gccgtaatgg
    35221 acgatgaagc gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggg agccgcaccg
    35281 aagtcttaag tgggcaacaa gccgcttgcg atatggctgc gcttgaggat gttgatcagg
    35341 tgatggcagc cattgttggc gctgctgggc tgttacctac gcttgctgcg atccgcgcgg
    35401 gtaaaaccat tttgctggcc aataaagaat cactggttac ctgcggacgt ctgtttatgg
    35461 acgccgtaaa gcagagcaaa gcgcaattgt taccggtcga tagcgaacat aacgctattt
    35521 ttcagagttt accgcaacct atccagcata atctgggata cgctgacctt gagcaaaatg
    35581 gcgtggtgtc cattttactt accgggtctg gtggcccttt ccgtgagacg ccattgcgcg
    35641 atttggcaac aatgacgccc gatcaagcct gccgtcatcc gaactggtcg atgggacgta
    35701 aaatttccgt cgattcggct accatgatga acaaaggtct ggaatacatt gaagcgcgtt
    35761 ggctgtttaa cgccagcgcc agccagatgg aagtgctgat tcacccgcag tcagtgattc
    35821 actcaatggt gcgctaccag gacggcagtg ttctggcgca gctgggtgaa ccggatatgc
    35881 gtacgccaat tgctcacacc atggcatggc cgaatcgcgt gaactctggc gtgaagccgc
    35941 tcgatttttg caaactaagt gcgttgacat ttgccgcacc ggattatgat cgttatccat
    36001 gtctgaaact ggcgatggag gcgtttgaac aaggccaggc agcgacgaca gcattgaatg
    36061 ccgcaaatga aatcaccgtg gctgcttttc ttgcgcaaca aatccgcttt acggatatcg
    36121 ccgcgttgaa tttatccgta ctggaaaaaa tggatatgcg cgaaccacaa tgtgtggatg
    36181 atgtgttatc tgttgatgcg aacgcgcgtg aagtcgccag aaaagaggtg atgcgtctcg
    36241 caagctgagg ataatccggc tacagagagt cgcgctattt gttagcgtag ggcttcagtg
    36301 atatagtctg cgccatctga tcgtaagtag ttggctttat aaggtcaggt atgccgtggt
    36361 tttacacggc ttttttttgt ataggcttca gtattcctga gtaccgtaaa ccctgtcagg
    36421 gaataagaaa cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat tgccagcgca
    36481 tggctgtcgt catgttgcga tcattatgga cggcaatggt cgctgggcaa aaaagcaagg
    36541 gaagattcgt gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg ctgtctcttt
    36601 tgcggccaac aacggtattg aggcgttaac gctgtatgcc tttagtagtg aaaactggaa
    36661 ccgaccagcg caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc tagatagcga
    36721 agtaaaaagt ctgcaccgac ataacgtgcg tctgcgtatt attggcgata ccagtcgctt
    36781 taactcgcgt ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg ggaataccgg
    36841 tctgacgctg aatattgcgg cgaactacgg tggacgttgg gatatagtcc agggagtcag
    36901 gcaactggct gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag atgaagagat
    36961 gctaaaccag catgtctgta tgcatgaact ggcccctgta gatttagtaa ttaggactgg
    37021 gggggagcat cgcattagta actttttgct ttggcaaatt gcctatgccg aactttactt
    37081 tacagatgtt ctctggcccg atttcgatga acaagacttt gaaggggcgt taaatgcctt
    37141 tgctaatcga gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag cctgatgggg
    37201 gtcgcttttg ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg tcgtcatcgc
    37261 ggcgttgttt ctgttgccgc cggtggggtt cgccattgta acgctggtgg tctgtatgct
    37321 ggcagcgtgg gaatggggac agcttagcgg ttttaccact cgttcgcagc gagtatggtt
    37381 ggcggtgtta tgcgggttat tgttggcgct gatgcttttt ctgttgccgg aatatcaccg
    37441 aaatattcat caaccgctgg ttgaaatctc actttgggct tcgctgggtt ggtggattgt
    37501 cgcgctattg ctggtgctgt tttaccctgg ttccgcagca atctggcgta actctaaaac
    37561 attgcgcatt atttttggcg tgctaaccat tgttcccttc ttctggggca tgctggcgtt
    37621 acgggcctgg cactacgacg agaatcatta cagtggcgca atatggctgc tctatgtcat
    37681 gatcctggta tggggcgctg actctggcgc atatatgttt ggcaaattgt ttggtaaaca
    37741 taaactggca ccgaaggttt ctccgggtaa aacctggcaa ggctttatcg gtggactcgc
    37801 gaccgcagcg gtaatctcat ggggttatgg catgtgggtg aatctcgacg tcgctcccgt
    37861 caccttactc atttgctcta ttgtcgcagc gttagcctca gtgctcggcg atctgaccga
    37921 aagtatgttt aagcgtgaag caggaattaa ggacagcggt catttaattc caggacacgg
    37981 tggtatttta gatcgtattg atagcctgac ggctgcggta ccggtctttg cttgcttgtt
    38041 gttactggta ttcaggacgc tttaacggaa ggtaatatgc tgagttttct ctgggatttg
    38101 gcttcgttca tcgttgcact gggtgtactt atcaccgtgc atgaatttgg tcatttctgg
    38161 gttgcccggc gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg taaggcgctc
    38221 tggcggcgaa ctgataagct cggcaccgaa tatgttatcg ccctgatccc gttgggcggt
    38281 tatgtcaaaa tgctggatga gcgcgcagaa ccggtcgttc cggaactccg ccaccatgcc
    38341 ttcaataata aatctgtcgg ccaacgagcg gcgattattg ccgcaggtcc ggttgcaaac
    38401 ttcatttttg ctatctttgc ctactggctg gtttttatta ttggtgtgcc tggcgtacgt
    38461 ccggtggttg gtgaaatagc agccaattcg atagctgcgg aagcacaaat tgcaccaggt
    38521 acggaactaa aagccgtaga tggtatcgaa acgcctgatt gggatgccgt gcgtttgcag
    38581 ttggtcgata aaattggcga tgaaagcacc accattacgg tagcgccatt tggcagcgac
    38641 caacggcggg atgtaaagct cgatttacgt cactgggcgt ttgagcctga taaagaagat
    38701 ccggtatctt cgttggggat tcgtcctcgt gggccgcaaa ttgaacctgt actggaaaat
    38761 gtgcagccaa actcggcggc aagcaaggca ggtttgcaag caggcgacag gatcgttaaa
    38821 gtcgatggtc agcccttaac gcagtgggtg acctttgtga tgcttgtccg ggataacccg
    38881 ggtaaatcct tagcgttaga aatcgaaagg caggggagtc ccttgtcttt gacattaatc
    38941 ccggagagta aaccgggtaa gggtaaagcg attggttttg tcggtattga gccgaaagtc
    39001 attcctttgc cagatgagta taaagttgta cgccagtatg ggccgttcaa cgctatcgtc
    39061 gaagccacgg acaaaacgtg gcagttgatg aagctaacgg tcagtatgct gggaaaattg
    39121 atcaccggtg atgtgaaact gaacaacctc agtgggccga tctctatcgc caagggggct
    39181 gggatgacag cggaactcgg ggtagtttat tacctgccgt ttcttgcgct tattagcgtg
    39241 aacttaggga taattaacct gtttccgttg cccgtacttg acggggggca tctgctgttc
    39301 cttgcgatcg aaaagatcaa gggcggaccg gtatccgagc gggttcaaga cttttgttat
    39361 cgcattggct cgattctgct ggtgctgtta atggggcttg cacttttcaa tgatttctct
    39421 cggttatgag agttagttag gaagaacgca taataacgat ggcgatgaaa aagttgctca
    39481 tagcgtcgct gctgtttagc agcgccaccg tatacggtgc tgaagggttc gtagtgaaag
    39541 atattcattt cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc agtatgccgg
    39601 tgcgcacagg cgacacggtt aatgatgaag atatcagtaa taccattcgc gctctgtttg
    39661 ctaccggcaa ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt ctggttcagg
    39721 taaaagaacg tccgaccatt gccagcatta ctttctccgg taacaaatcg gtgaaagatg
    39781 acatgctgaa gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc ctcgatcgca
    39841 ccaccattgc cgatatcgag aaaggtctgg aagacttcta ctacagcgtc ggtaaatata
    39901 gcgccagcgt taaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac ctaaaactgg
    39961 tgttccagga aggtgtgtca gctgaaatcc agcaaattaa cattgttggt aaccatgctt
    40021 tcaccaccga tgaactgatc tctcatttcc aactgcgtga cgaagtgccg tggtggaacg
    40081 tggtaggcga tcgtaaatac cagaaacaga aactggcagg cgaccttgaa accctgcgca
    40141 gttactatct ggatcgcggt tatgcccgtt tcaacatcga ctctacccag gtcagtctga
    40201 cgccagataa aaaaggtatt tacgtcacgg tgaacatcac cgaaggcgat cagtacaagc
    40261 tttctggcgt tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt gagcagctga
    40321 ctaagatcga gccgggcgag ctgtataacg gcaccaaagt gaccaaaatg gaagatgaca
    40381 ttaaaaagct tcttggtcgc tatggttatg cctatccgcg cgtacagtcg atgcctgaaa
    40441 ttaacgatgc cgacaaaacc gttaaattac gcgtgaacgt tgatgcgggt aaccgtttct
    40501 acgtgcgtaa gatccgtttt gaaggtaacg atacctcgaa agatgccgtc ctgcgtcgcg
    40561 aaatgcgtca gatggaaggt gcatggctgg ggagcgatct ggtcgatcag ggtaaggagc
    40621 gtctgaatcg tctgggcttc tttgaaactg tcgataccga tacccaacgt gttccgggta
    40681 gcccggacca ggttgatgta gtctacaagg tgaaagaacg taacaccggt agcttcaact
    40741 tcgggattgg ttacggtact gaaagtggcg tgagcttcca ggctggtgtg caacaggata
    40801 actggttagg tacgggttat gctgttggta tcaacgggac caaaaacgat taccagacct
    40861 atgctgaact gtcggtaacc aacccgtact tcaccgtaga tggcgtaagc ctcggtggtc
    40921 gtctcttcta taatgacttc caggcagatg acgccgacct gtccgactat accaacaaga
    40981 gttatggtac agatgtgacg ttgggcttcc cgatcaacga atataactcg ctgcgtgcag
    41041 gtctgggtta tgtacataac tccctgtcca acatgcagcc tcaggttgcg atgtggcgct
    41101 atctgtactc tatgggtgaa catccgagca cctctgatca ggataacagc ttcaaaacgg
    41161 acgacttcac gttcaactat ggttggacct acaacaagct tgaccgtggt tacttcccga
    41221 cagatggttc acgtgtcaac ctgaccggta aagtgaccat tccgggatcg gataacgaat
    41281 actacaaagt gacgttagac acggcgactt atgtgccgat tgatgatgat cacaaatggg
    41341 ttgttctggg tcgtacccgc tggggttatg gtgatggttt aggcggcaaa gagatgccgt
    41401 tctatgagaa cttctatgcc ggtggttcca gcaccgtgcg tggcttccag tccaatacca
    41461 ttggtccgaa agcagtttac ttcccgcatc aggccagtaa ttatgatccg gactatgatt
    41521 acgaatgtgc gactcaggac ggcgcgaaag atctgtgtaa atcggatgat gctgtaggcg
    41581 gtaacgccat ggcggttgcc agcctcgagt tcatcacccc gacgccgttt attagcgata
    41641 agtatgctaa ctcggttcgt acttccttct tctgggatat gggtaccgtt tgggatacaa
    41701 actgggattc cagccaatat tctggatatc cggactatag tgatccaagc aatatccgta
    41761 tgtctgcggg tatcgcatta caatggatgt ccccattggg gccgttggtg ttctcctacg
    41821 cccagccgtt caaaaagtac gatggagaca aggcagaaca gttccagttt aacatcggta
    41881 aaacctggta agtgttctcc acaaaggaat gtagtggtag tgtagcgatg actttaggcg
    41941 atcaatataa gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa atgggatggt
    42001 aaggagttta ttgtgaaaaa gtggttatta gctgcaggtc tcggtttagc actggcaact
    42061 tctgctcagg cggctgacaa aattgcaatc gtcaatatgg gcagcctgtt ccagcaggta
    42121 gcgcagaaaa ccggtgtttc taacacgctg gaaaacgagt tcaaaggccg tgccagcgaa
    42181 ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc catgaaagcg
    42241 ggcagcgatc gcactaagct ggaaaaagac gtgatggctc agcgccagac ttttgctcag
    42301 aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca acgaagaacg cggcaaactg
    42361 gttactcgta tccagactgc tgtgaaatcc gttgccaaca gccaggatat cgatctggtt
    42421 gttgatgcaa atgccgttgc ttacaacagc agcgatgtaa aagacatcac tgccgacgta
    42481 ctgaaacagg ttaaataagt aatgccttca attcgactgg ctgatttagc gcagcagttg
    42541 gatgcagaac tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc catgcaatct
    42601 gcacaaacag gtcacattac gttcatggtt aaccccaaat accgtgagca tttaggcttg
    42661 tgccaggcgt ccgcggttgt catgacccag gacgatcttc ctttcgcgaa aagtgccgcg
    42721 ctggtagtga agaatcccta cctgacttac gcgcgtatgg cgcaaatttt agataccacg
    42781 ccgcagcccg cgcagaacat tgcacccagt gcggtgattg acgcgacggc gaagctgggt
    42841 aacaacgtat cgattggcgc taacgcggtg attgagtccg gcgttgaact gggcgataac
    42901 gtgattatcg gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc aggttcgcgt
    42961 ctctgggcga acgtaaccat ttaccatgag atccagatcg gtcagaattg cctgatccag
    43021 tccggaacag tggtaggcgc agacggcttt ggttatgcca acgatcgtgg taactgggtg
    43081 aagatcccac agattggtcg cgtaattatt ggcgatcgcg tggagatcgg tgcctgcact
    43141 accattgatc gtggcgcgct ggatgacact gttattggca atggtgtgat cattgataac
    43201 cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata cggcagttgc cggtggcgtc
    43261 attatggcgg gcagcctgaa aattggtcgt tactgcatga tcggcggagc cagcgtaatc
    43321 aacgggcata tggaaatatg cgacaaagtg acggttacgg gcatgggtat ggtgatgcgt
    43381 cccatcactg aaccaggcgt ctattcctca ggcattccgc tgcaacccaa caaagtctgg
    43441 cgcaaaaccg ctgcactggt gatgaacatt gatgacatga gcaagcgtct gaaatcgctt
    43501 gagcgcaagg ttaatcaaca agactaacgt tccatctttt gttcgccaaa ctttacggcc
    43561 tgtctcattc ttacgattgc ggcaggccgt gttattattg tcgtttctta tattttgaca
    43621 ggaagagtat cttgactact aacactcata ctctgcagat tgaagagatt ttagaacttc
    43681 tgccgcaccg tttcccgttc ttactggtgg atcgcgtgct ggattttgaa gaaggtcgtt
    43741 ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt cttccagggc catttccctg
    43801 gaaaaccgat tttcccgggt gtgctgattc tggaagcaat ggcacaggca acaggtattc
    43861 tggcgtttaa aagcgtagga aaactggaac cgggtgagct gtactacttc gccggtattg
    43921 acgaagcgcg ctttaagcgc ccggtcgtgc ctggcgatca aatgatcatg gaagtcactt
    43981 tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc gacggtaaag
    44041 tagtttgcga agcaacgatg atgtgtgctc gtagccggga ggcctgatac gtgattgata
    44101 aatccgcctt tgtgcatcca accgccattg tggaagaggg cgcgtcgatt ggcgcgaacg
    44161 ctcacattgg tcctttttgt atcgttggac cccatgtcga aattggtgag ggtaccgtac
    44221 tgaaatctca cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat gagatttatc
    44281 agttcgcctc catcggcgaa gttaaccagg atctgaaata tgctggcgaa ccgacccgcg
    44341 tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt ggcacagtcc
    44401 agggcggtgg attgacgaag gtgggcagcg acaacttact gatgatcaat gcgcacattg
    44461 cgcacgattg tacggtaggt aaccgctgca tcctcgccaa caacgcaacg ctggcgggtc
    44521 acgtatcggt tgacgacttc gcgatcatcg gcggcatgac cgcagtccat cagttctgca
    44581 tcattggtgc gcatgtgatg gttggcggct gctccggtgt ggcgcaggac gtccctcctt
    44641 atgtcattgc gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa gggctgaagc
    44701 gccgcggatt cagccgtgag gcgattaccg ctatccgcaa tgcgtataag ctgatttatc
    44761 gtagcggtaa aacgctcgat gaagtgaaac cggaaattgc tgaactggcg gaaacatatc
    44821 cggaagtgaa agcctttacc gatttctttg cacgctcaac gcgcggtctg attcgttaat
    44881 gactgaacag cgtccattaa cgattgccct ggtcgccgga gaaacctccg gcgatatcct
    44941 gggggccggt ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct ttgttggtgt
    45001 tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg aagaactggc
    45061 agtgatgggc attgttgaag tgctcggtcg tctgcgtcgc ttactacata ttcgtgccga
    45121 tctgacaaag cgttttggcg aactgaagcc agatgttttt gttggtattg atgcgcctga
    45181 cttcaatatt actcttgaag gtaacctcaa aaagcagggt atcaaaacca ttcattacgt
    45241 cagtccgtcc gtctgggcgt ggcgacagaa acgcgttttc aaaataggca gagccaccga
    45301 tctggtgctc gcatttctgc ctttcgaaaa agcgttttat gacaaataca acgtaccgtg
    45361 ccgctttatc ggtcatacca tggctgatgc catgccatta gatccagata aaaatggtgc
    45421 ccgtgatgtg ctggggatcc cttacgatgc ccactgtctg gcattgttgc cgggcagccg
    45481 tggtgcagaa gtcgaaatgc ttagtgccga tttcctgaaa acggcccagc ttttgcgcca
    45541 gacatatccg gatctcgaaa tcgtggtgcc gctggtgaat gccaaacgcc gcgagcagtt
    45601 tgaacgcatc aaagctgcag tcgcgccaga cctgtcggtt catttgctgg atggaatggg
    45661 ccgtgaggcg atggtcgcca gtgatgcggc gctactggcg tcgggtacgg cagcactgga
    45721 gtgtatgctg gctaaatgcc cgatggtagt gggatatcgc atgaagcctt tcaccttctg
    45781 gctggcgaag cgactggtga aaactgatta tgtctcgctg ccaaatctgc tggcgggcag
    45841 agagttagtc aaagagttat tgcaggaaga gtgtgagccg caaaaattgg ctgcggcgct
    45901 gttaccgctg ttggcgaacg ggaaaaccag ccatgcgatg cacgacacct tccgcgagct
    45961 gcatcagcag atccgctgca atgccgatga gcaggcggca caagccgttc tggagttagc
    46021 acaatgatcg aatttgtcta tccgcacacg cagctggttg cgggtgtgga tgaagtcgga
    46081 cgcgggccgt tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc ggcgcgccca
    46141 attgccgggc tgaatgattc caaaaagctg agcgaaaaac gccgtctggc gctctgtgaa
    46201 gagatcaaag agaaagcgtt gagctggagt ctgggccgtg cggaacctca cgaaatcgac
    46261 gaactgaata ttttacatgc gactatgctg gcgatgcagc gtgccgtcgc tggactgcat
    46321 attgcgccgg aatatgtgtt gattgatggt aaccgctgcc cgaaattacc gatgccttcg
    46381 atggctgtgg tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc tatcctggcg
    46441 aaagtgacgc gtgacgccga aatggcggcg ctggatattg ttttcccgca atatggtttt
    46501 gcccaacaca aagggtatcc aaccgctttt catctggaaa aactggctga acacggcgcg
    46561 accgaacacc atcggcgcag ctttgggcct gtcaaacgcg cactgggact tgcgtcctga
    46621 ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt ctgaaccacg tttcgtacac
    46681 ctgcgggtgc acagcgacta ctcgatgatc gatggcctgg ccaaaaccgc gccgctggta
    46741 aaaaaggcgg cggcgttggg tatgccagca ctggcgatca ccgatttcac caacctttgc
    46801 ggtctggtga agttctacgg agcgggacat ggcgcaggga ttaagcccat cgtcggggcg
    46861 gattttaacg tccagtgcga cctgctgggt gatgagttaa ctcacctgac ggtgctggcg
    46921 gcgaacaata ccggctatca gaatctgacg ttgctgatct caaaagcgta tcagcgcggg
    46981 tacggtaccg ccgggccgat catcgatcgc gactggctta tcgaattaaa cgaagggctg
    47041 atcctccttt ccggcgggcg catgggcgac gtcgggcgca gccttttgcg tggtaacagc
    47101 gcgctggtag atgagtgcgt tgcgttttat gaagagcact tcccggatcg ctattttctc
    47161 gagctgatcc gcaccggcag gccggacgaa gaaagttatc tgcacgcggc agtggaactg
    47221 gcggaatcac gcggtttgcc cgttgtggcg accaacgacg tgcgctttat cgacagcagc
    47281 gactttgacg cacacgaaat ccgcgtcgcg atccacgacg gctttaccct cgacgatcct
    47341 aaacgcccgc gtaactattc gccgcagcaa tatatgcgta gcgaagagga gatgtgcgag
    47401 ctttttgccg acattcccga agcccttgcc aacaccgttg agatcgccaa acgttgtaac
    47461 gtaactgtgc gtctgggtga atacttcttg ccgcaattcc cgaccgggga catgagtacc
    47521 gaagattatc tggtcaagcg tgcaaaagaa ggcctggaag agcgtctggc ctttttattc
    47581 cctgacgagg aagaacgtct taagcgtcgt ccggagtatg acgaacgtct ggatactgaa
    47641 cttcaggtta tcaaccagat gggcttcccg ggttacttcc tcatcgttat ggaatttatc
    47701 cagtggtcga aagataacgg tgtaccggta gggcctggcc gtggttctgg tgcagggtca
    47761 ctggtggcct acgcgttaaa aatcaccgat ctcgatccgc tggaatttga cctgctgttc
    47821 gaacgtttcc ttaacccgga acgtgtctcc atgcctgact tcgacgttga cttctgtatg
    47881 gagaaacgtg accaggttat cgaacatgtg gcggacatgt acggtcgcga tgcggtatcg
    47941 cagattatca ccttcggtac gatggcggcg aaagcggtga tccgcgacgt aggccgtgta
    48001 ttggggcatc cgtacggatt tgtcgatcgt atctcgaaac tgatcccgcc cgatccgggt
    48061 atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta cgaagcggat
    48121 gaagaagtta aggcgctgat cgacatggcg cgcaaactgg aaggggtcac ccgtaacgcc
    48181 ggtaagcacg ccggtggggt ggttatcgcg ccgaccaaaa ttactgattt tgcgccgctt
    48241 tattgcgatg aagagggcaa acatccggtc acccagtttg ataaaagcga cgttgaatac
    48301 gccgggctgg tgaagttcga cttcctcggt ttgcgtacgc tcaccatcat caactgggcg
    48361 ctggagatga tcaacaagcg gcgggcgaag aatggcgagc cgccgctgga tatcgccgcg
    48421 atcccgctgg atgacaagaa aagcttcgac atgctgcaac gctcggaaac cacggcggta
    48481 ttccagcttg aatcgcgcgg catgaaggac ctgatcaagc gtctgcaacc tgactgcttc
    48541 gaagatatga tcgcactggt ggcactgttc cgccccggcc cgttgcaatc agggatggtg
    48601 gataacttta tcgaccgtaa acatggtcgc gaagagatct cctatccgga cgtgcagtgg
    48661 cagcatgaaa gcctgaaacc ggtactggag ccaacctacg gcatcatcct gtatcaggaa
    48721 caggtcatgc agattgccca ggtgctttct ggttataccc tcggtggcgc ggatatgctg
    48781 cgtcgtgcga tgggtaagaa aaagccggaa gagatggcta agcagcgttc tgtatttgct
    48841 gaaggtgcag aaaagaacgg aatcaacgcc gaactggcga tgaaaatctt cgacctggtg
    48901 gagaaattcg cgggttacgg atttaacaaa tcgcactcgg cggcctatgc tttggtgtca
    48961 tatcaaacgt tatggctgaa agcgcactat ccggcggagt ttatggcggc ggtaatgacc
    49021 gccgatatgg acaacaccga gaaggtggtg ggcctggtgg atgagtgctg gcggatgggg
    49081 ctgaaaatcc tgccaccaga tataaactcc ggtctttacc atttccacgt caacgacgac
    49141 ggcgaaatcg tgtatggtat tggcgcgatc aaaggggtag gtgaaggccc gattgaggcc
    49201 atcatcgaag cccgtaataa aggcggctac ttccgcgaac tgtttgatct ctgcgcccgt
    49261 accgacacca aaaagttaaa ccggcgggtg ctggaaaaac tgatcatgtc cggggcgttt
    49321 gaccgtcttg ggccacaccg cgcggcgctg atgaactcgc tgggcgatgc gttaaaagcg
    49381 gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg cgtgctggcc
    49441 gaagagccgg aacaaattga acaatcctac gccagctgcc aaccgtggcc ggagcaggtg
    49501 gtattagatg gggaacgtga aacgttaggt ctgtacctga cgggacaccc tatcaaccag
    49561 tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat gcacccgaca
    49621 gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt tatggtcacc
    49681 aagcgcggca atcgtatcgg tatctgcacg ctggatgacc gttccgggcg gctggaagtg
    49741 atgttgttta ctgacgccct ggataaatac cagcaattgc tggaaaaaga ccgcatactt
    49801 atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat gaccgctcgc
    49861 gaagtgatgg atattgacga agcccgggaa aaatatgctc gcgggcttgc tatctcgctg
    49921 acggacaggc aaattgatga ccagctttta aaccgactcc gtcagtctct ggaaccccac
    49981 cgctctggga caattccagt acatctctac tatcagaggg cggatgcacg cgcgcgattg
    50041 cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt tattaaacga tctccgtggc
    50101 ctcattggtt cggagcaggt ggaactggag tttgactaat acaggaatac tatgagtctg
    50161 aatttccttg attttgaaca gccgattgca gagctggaag cgaaaatcga ttctctgacc
    50221 gcggttagcc gtcaggatga gaaactggat attaacatcg atgaagaagt gcatcgtctg
    50281 cgtgaaaaaa gcgtagaact gacgcgtaaa atcttcgccg atctcggtgc atggcagatt
    50341 gcgcaactgg cacgccatcc acagcgtcct tataccctgg attacgttcg cctggcattt
    50401 gatgaatttg acgaactggc tggcgaccgc gcgtatgcag acgataaagc tatcgtcggt
    50461 ggtatcgccc gtctcgatgg tcgtccggtg atgatcattg gtcatcaaaa aggtcgtgaa
    50521 accaaagaaa aaattcgccg taactttggt atgccagcgc cagaaggtta ccgcaaagca
    50581 ctgcgtctga tgcaaatggc tgaacgtttt aagatgccta tcatcacctt tatcgacacc
    50641 ccgggggctt atccgggcgt gggcgcagaa gaacgtggtc agtcggaagc cattgcacgc
    50701 aacctgcgtg aaatgtctcg cctcggcgta ccggtagttt gtacggttat cggtgaaggt
    50761 ggttctggtg gtgcgctggc gattggcgtg ggcgataaag tgaatatgct gcaatacagc
    50821 acctattccg ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag cgctgacaaa
    50881 gcgccgctgg cggctgaagc gatgggtatc atcgctccac gtctgaaaga gctgaaactg
    50941 atcgactcca tcatcccgga accgctgggc ggtgctcacc gtaacccgga agcgatggcg
    51001 gcgtctctga aagcgcagct gctggcggat ctggccgatc tcgacgtgtt aagcactgaa
    51061 gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg gctacgcgta atcggcaaat
    51121 gttctgaaaa gggtcacttc ggtggccctt ttttatcgcc acggtttgag caggctatga
    51181 ttaaggaagg attttccagg aggaacacat gaacatcatt gccattatgg gaccgcatgg
    51241 cgtcttttat aaagatgagc ccatcaaaga actggagtcg gcgctggtgg cgcaaggctt
    51301 tcagattatc tggccacaaa acagcgttga tttgctgaag tttatcgaac ataacccacg
    51361 aatttgcggc gtgatttttg actgggatga gtacagtctc gatttatgta gcgatatcaa
    51421 tcaacttaat gaatatctcc cgctttatgc cttcatcaac acccactcga cgatggatgt
    51481 cagcgtgcag gatatgcgga tggcgctctg gttttttgaa tatgcgctgg ggcaggcgga
    51541 agatatcgcc attcgtatgc gtcagtatac caacgaatat cttgataaca ttacgccgcc
    51601 gttcacgaaa gccttgttta cctacgtcaa agagcggaag tacacctttt gtacgccggg
    51661 gcatatgggc ggcaccgcat atcaaaaaag cccggttggc tgtctgtttt atgatttttt
    51721 cggcgggaat accctcaagg cggacgtctc tatttcggtc accgagcttg gttcgttgct
    51781 cgaccacacc gggccacacc tggaagcgga agagtacatc gcgcggacgt ttggcgcgga
    51841 acagagttat atcgttacca acggaacatc gacgtcgaac aaaattgtgg gtatgtacgc
    51901 cgcgccatcc ggcagtacgc tgttgatcga ccgcaattgt cataaatcgc tggcgcatct
    51961 gttgatgatg aacgatgtag tgccagtctg gctgaaaccg acgcgtaatg cgttggggat
    52021 tctgggtggg atcccgcgcg gtgaatttac tcgcgacagc atcgaagaga aagtcgctgc
    52081 caccacgcag gcacaatggc cggttcatgc ggtgatcacc aactccacct atgatggctt
    52141 gctctacaac accgactgga tcaaacagac gctggatgtc ccgtcgattc acttcgattc
    52201 tgcctgggtg ccgtacaccc attttcatcc aatctaccag ggtaaaagtg gtatgagcgg
    52261 cgagcgtgtt gcaggaaaag tgatcttcga aacgcaatct acccacaaaa tgctggcggc
    52321 gttatcgcag gcttcgctga tccacattaa aggcgagtat gacgaggagg cgttcaacga
    52381 agcctttatg atgcatacca ccacctcgcc cagttatccc attgttgctt ctgttgagac
    52441 ggcggcggcg atgttgcgtg gtaatccggg caaacggctg attaaccgtt cagtagaacg
    52501 agctctgcat tttcgcaaag aggtccagcg gctgcgggaa gagtctgacg gctggttttt
    52561 cgatatctgg caaccgccgc aggtggatga agccgaatgc tggcccgttg cgcctggcga
    52621 acagtggcac ggctttagcg atgcggatgc caatcacatg tttctcgatc cggttaaagt
    52681 cactattttg acaccgggga tggacgagca gggcaatatg agcgaggagg ggatcccggc
    52741 ggcgctggtg gcaaaattcc tcgacgaacg tgggatcgta gtagagaaaa ccggccctta
    52801 taacctgctg tttctcttta gtattggcat cgataaaacc aaagcaatgg gattattgcg
    52861 tgggttgacg gaatttaagc gctcttacga tctcaacctg cggatcaaaa atatgctgcc
    52921 cgatctctat gcagaagatc ccgatttcta ccgcaatatg cgtattcagg atctggcgca
    52981 agggatccat aagctgattc gtaaacacga tcttcccggt ttgatgttgc gggcatttga
    53041 taccttgccg gagatgatca tgacgccaca tcaggcatgg cagagacaga ttaaaggcga
    53101 agtagaaacc attgcgctgg aacaactggt cggtagagta tcggcaaata tgatcctgcc
    53161 ttatccaccc ggcgtaccgc tgctgatgcc gggagaaatg cttaccgaag agagccgcac
    53221 ggtactcgat tttctactga tgctttgttc cgtagggcaa cattaccccg gttttgaaac
    53281 ggatattcac ggcgcgaaac atgacgaaga cggtgtttac cgcgtacgag tcctaaaaat
    53341 ggcaggataa cttgccagag cggcttccgg gcgagtaacg tgctgttaac aaataaagga
    53401 gacgttatgc tgggtttaaa acaggttcac catattgcga ttattgcgac ggattatgcg
    53461 atgagcaaag ctttctactg cgatattctt ggtttcacgc tgcaaagcga agtctatcgc
    53521 gaagcgcgcg actcgtggaa aggggatttg gcgcttaatg ggcaatatgt gattgagctt
    53581 ttctcatttc cgttcccgcc ggagcgcccc agccgaccgg aagcttgcgg cctgcgtcat
    53641 ctggctttta gcgttgatga tatcgatgcg gcagtggcgc accttgaaag ccataacgtg
    53701 aagtgtgaag ccatccgtgt cgatccctac acgcaaaaac gcttcacgtt ctttaacgat
    53761 ccggacgggc tgccgttgga actgtatgag cagtaaggct tgtcatcgcc gcatttgccc
    53821 ggtaacgtgc ccggcattgc tactgtaaaa tcgcaccatc atgacactca cgctcaatag
    53881 acaacttctc tcctcacgcc agattctggt ggcctttagc ggcggccttg attctactgt
    53941 cttactgcat caactggtgc agtggcggac ggaaaatccg ggcgtcactc tgcgcgctat
    54001 ccatgtgcat cacggtttaa gcgccaatgc tgatgcctgg gtgaaacatt gtgaaaacat
    54061 ctgccaacag tggcaggtgc cgctggtggt cgaacgtgtg cagcttgcgc aagaggggct
    54121 aggtattgag gcccaggcgc ggcaggcacg ttatcaggca tttgcccaca ccttgttgcc
    54181 cggtgaagtg ctggttacag cgcaacatct cgacgatcaa tgtgaaacct ttctgctggc
    54241 gctaaaacgc ggtagcggcc ctgccgggct ttcggctatg gcggaagttt cggagtttgc
    54301 cggaacacag cttatccgcc cgttgcttgc ccgcacgcgg ggggaactgg tgcagtgggc
    54361 gttggcgcat ggtttacgct ggattgaaga cgaaagtaat caggatgata gctacgatcg
    54421 taactttctg cgcctgcgcg tagtgccgtt attgcagcag cgttggccgc attttgccga
    54481 agcaacagcc cgcagcgccg cactttgcgc tgaacaagag agcctgttag atgaactgct
    54541 ggcggatgat ttagcgcact gtcaaactcc gcaggggacg ctgcagattg cgctaatgct
    54601 ggcgatgagt gatgcccgtc gcgcggcgat tatccgccgc tggctggcag ggcagaatgc
    54661 accgatgcct tcccgcgacg cgttggtgag gatctggcag gaagtggcgc tggcgcggga
    54721 agatgcctcc ccctgtttac gtttgggcgc gtttgaaatc cgccgctatc agtcgcaact
    54781 atggtggatt aaatccgttg ccgggcaaag cgaaaccatt gtgccgtggc agacgtggct
    54841 acaaccgctg gaattaccgg cggggctggg aagtgtacag cttactgcgg gaggcgatat
    54901 tcgccctccg cgtgcagacg aagcggtcag cgtgcgtttc aaagcgccag gattgctgca
    54961 tattgtcggg cgcaacggcg gacgtaagct gaagaaaatc tggcaagagt taggcgttcc
    55021 gccgtggcta cgtgacacca cgccactgct gttttatggc gaaacgctga ttgcggcggc
    55081 aggggtattt gtgacgcaag aaggtgtggc tgtaggtgag aatggcgtca gttttgtctg
    55141 gcagaaaacg cttagtcaag tgaaagccgg ataagacgca tcaaacgtcg catccggcga
    55201 aagtcaatca ggactcgctc accaccaccg taccgatttc cgggtggcta aagctggtaa
    55261 ttttatccag acgcagctcg cgggttgcac cagcggcctc gacgaccaga tactccacat
    55321 ttttgcggga gactaaatcg ctggccttcg cctgcaattt ttcgccatct ttcagctcaa
    55381 gtgtcagcat taaatgatgc tggcaggcga gctcaagatt atcgtaatca tcacaattga
    55441 ttggttgata cgtatcattc attgacataa tcgctcacca gtaagtttgc cgcagcgtat
    55501 gctgcttttt ccctgacagc ctcagaaagg gcgtcgtcgg cagccatttc attcagcact
    55561 ttcaaaacgc agcccagcgc gtccggaacg tatcctaagt ctccgctggc gatttccgcg
    55621 taccgcttgc gtattaactc acaatatttt tccacatgcc ctcctgtcag cactctgact
    55681 taaccgtgga tgcaagtcta agcctacgaa gataaactct gtttcgcaag gtgactatac
    55741 cacactcatt tctgcaatat cagcgccgca actgcacgta ttccgttaca atggcctcct
    55801 gattcgaaag gagttttctt atggcgctta aagcgacaat ttataaagcg acggttaatg
    55861 tggccgatct cgaccgcaac cagtttctcg atgcctctct gacgctggcg cgccatcctt
    55921 cagaaaccca ggagcgcatg atgctgcgcc tgctggcgtg gctaaaatat gccgatgaac
    55981 gtctgcaatt tacccgtggt ttgtgtgccg atgatgagcc ggaagcgtgg ctgcgtaacg
    56041 atcacctggg cattgatttg tggattgaac tggggctgcc ggatgagcgg cggattaaga
    56101 aagcctgcac ccaggccgca gaagtggcgc tgtttgccta taatagtcgg gcggcgcaaa
    56161 tctggtggca gcaaaatcag agcaaatgtg cgcagtttgc caatctttcc gtctggtatc
    56221 tggacgacga gcagctggcg aaagtgagcg cctttgccga tcgtaccatg acgctgcagg
    56281 caacgattca agatggtgtt atctggttat cggatgataa gaataatctg gaagtgaact
    56341 taaccgtctg gcaacaacct tcatgattgt gatttcccga catgttgcta tctctgatgg
    56401 cgagtttgag atcaccgcca ttcgtgcgca gggcgcgggc gggcagcatg ttaataagac
    56461 ctcaacggct attcatctgc gttttgacat tcgggcgtcc agcctgccag agtattacaa
    56521 agaacgtctg ctcgccgcca gccatcactt gatcagcagt gatggggtga ttgtcattaa
    56581 ggcacaggaa taccgcaatc aggagctcaa ccgcgaagcg gcgctggccc ggctggtggc
    56641 agtgattaaa gatttaacaa cagaacaaaa agcccgacga cccacgcggc ccacccgcgc
    56701 atcaaaagag cgcaggctgg catcgaaagc acaaaaatca agcgtgaagg cgatgcgcgg
    56761 caaagtgcgc agcggtcggg aataaaaaga aggaatggat ggtgaaaaaa gcgatagtga
    56821 cagcgatggc tgtaatcagc ctctttactc tgatgggatg taataatcgg gccgaagtcg
    56881 atacgctttc tccggcacag gctgccgaac tgaaaccgat gccgcaaagt tggcgtggcg
    56941 tgctgccgtg tgctgattgc gaaggaatcg aaacctctct gttcctcgaa aaagacggaa
    57001 cctgggtgat gaatgagcgt tatctggggg ctcgtgaaga accttcctcc ttcgcttcct
    57061 acggtacatg ggcgcgaacc gctgacaagc tggtattaac cgatagcaaa ggtgaaaagt
    57121 catattatcg cgcgaaagga gatgcgctgg agatgctcga tcgtgaaggt aatccgattg
    57181 aatcgcagtt caactacacg ctggaaccgg cacaatccag cttacccatg acgccgatga
    57241 ccctgcgggg catgtatttt tatatggctg atgcggcgac cttcactgat tgcgcgaccg
    57301 gaaaacgttt catggtggcg aataacgcag agctggagcg tggctacctg gctgcgcgca
    57361 gtaacagtga aaaaccggtg ttactgtcag tagaaggtca ctttacgctt gaggctaatc
    57421 cggataccgg tgcgccgacc aaagtattag cgcccgatac ggcaggtaaa ttttacccaa
    57481 accaggattg cagtagtttg gggctgtaac ccgtcttgag acagaaacaa atgaaatatt
    57541 gcctcatgcg ctgcgcttat cagtcctaaa gcatacaatg caatattttg aattagcaca
    57601 attttgtagg ccggataagg cgttcacgcc gcatccggca ttttgcgcac gaggctggct
    57661 tttatcactg agtcagccca acaaagcggc ctgccttgat ataaatccca ggcttcatgt
    57721 gcccaacata ctgtaactgc gtttctggct taaacgcaga gatatcgcca gtacgcatat
    57781 gcatcagatc ggcagggcta atccagcctg attgcgccag cgtaagcgga tgcccggctt
    57841 tggcaaatgc atcagtgaca aattccgaac aaaaccacga ctttttgtct ccttcgccca
    57901 cactgcttaa ctgcggtttc gccaggccgc tgacgcactg ttgacgaaaa tcctcggaga
    57961 acggatttag tgagcacatc tggcgagtca ccataaatgg aataaattcg acaatgccgc
    58021 gatagttgta accgctatct ttaattttgt tggcgaacgc ggtgatttct gtggcttgtt
    58081 gcggggtaag atccgggact cgtaagacga aaagcttatc actatgcttt atggcttttt
    58141 taagggaaac aatctggacg ccagcgcctg tcgcttcggc aacgttatta tctcccaaat
    58201 agattgcaac gtgactcaca gaggaagtgc tgaatacgcg gatatcaaat gaggttaccc
    58261 caaggcttga ggagaaaagc aaatcgccgg gtttgagatc tggttccgtt atttctttta
    58321 ttgattgttc ggtgaaagag cttggatgct ggaatttaac agcccatgtt ttcgcctcgg
    58381 catttaccac tgtggctgat gagtctggct ggctgatatc aacggtacag gccgaaagta
    58441 aaagaaagca ggggagaagc aggcggcagt acgcctttgg tttatccatt ttatacaatc
    58501 catgtaaaaa agggccccga aattcaggac cctttctggc atcagccttt aatctgtttc
    58561 accagataat cgacgatgtc accagtctta attaactgtt tctcgccgtt acgacgatat
    58621 ttatattcga tatcatcgtt gtcgaggtta cggtcgccca gcacaatagt gtgcggaata
    58681 ccgatcagtt ccatatcagc aaacatcacg cccggacgct ctttgcggtc atccagcagc
    58741 acttcgatac cttgtgcgcg cagttcgctg tacagtttct cagcaagttc ctgtacgcgg
    58801 aaggatttgt gcatgttcat tggcagaatc gccaccagga acggcgcgat agcgtcaggc
    58861 catacgatgc cgcgttcgtc gtagttctgc tcaatcgccg ctgctaccac acgcgttacg
    58921 ccgataccgt agcaacccat cgtcaggatt tggttacggc catcttcacc ctgtacggag
    58981 gctttcagtg cttcggagta cttggtaccc agctggaaga tgtgaccgac ttcgatacca
    59041 cgtttgatca gcagcgtacc ctgaccatcc gggcttggat cgccagccac cacgttgcgg
    59101 atatcagcaa tttccggggt agcgacatcg cgatcccagt tgataccgaa gtagtgttta
    59161 ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg caacggtacg gtcaatcacc
    59221 accggaatcg gcatgtttac cggacccagt gaacccggac cggctttaac cacggcacga
    59281 atttcttctt cggtcgcaaa agtcagcggg ctggcaacct gcggcagttt ttctgcttta
    59341 acttcgttca gctcgtggtc accgcgcacc agcagcgcaa ccagcgggaa gctactgcct
    59401 tcaacggctt taaccagcag agtcttaacc gttttctcaa tcggcagatt gaactgttca
    59461 accagttccg cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc ctgggtagca
    59521 gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt caatgttcgc tgcatagtca
    59581 gaagtatcgg agaagaccac atcgtcttca ccgctctgcg ccagcacctg aaattcgtga
    59641 gaggcgctac cgccgataga accggtatcg gcctgtacgg cgcggaaatc cagccccatg
    59701 cggctgaaga ttttgctgta ggccgcatac attgcatcgt aggtttcctg cagggattcc
    59761 tgagaagtat ggaaagagta agcatctttc atcaggaatt cgcgggaacg catgacgcca
    59821 aaacgcggac gcacttcgtc gcggaacttg gtctggatct gatagaagtt cagcggcagc
    59881 tgtttgtaag agctcagctc gttacgaatc aggtctgtaa tcacttcttc atgagttggg
    59941 ccgagtacga acggacggtc accacggtca gcaatacgca gcaattccgg gccatactgt
    60001 tcccaacgac cactctcttg ccacagttca gatggctgaa ccaccggcat taacacctcg
    60061 atcgcaccgg cgttgttcat ctcttcacgc acgatgtttt cgactttttt cagaacgcgc
    60121 acgccggtcg gcagccaggt atataacccg gaggccagct tgcggatcat cccggcgcgc
    60181 agcatcagct gatggctgat cacctcggcg tcggcaggtg tctccttgag agtggagagc
    60241 aggtattggc tagtacgcat gttgttacgg ttccagttgg aaggtagaac aggctcaagg
    60301 cgagcctggg acaaaaaaag tgatttagtt taccagtgca aaagaaatgt caaaagagaa
    60361 gggcgtgaat ttaacgcggt tccagcgcaa agacttcaaa acctgcgtcg gtgacgcgcc
    60421 agcgaacgtt aaaatcatgc agccagacgg cataggtttt gcccgtttcc tcacctttac
    60481 gataggccgg gcgcgggtcc tgcgccagta cttcgcggat aaacagcgtt aactgcggat
    60541 aacgcttctt cagcgtcaaa agctgctttt cgacctctgc ggtaaaacac accgccattt
    60601 ctgccgctgg cgcactttgc gcatagctgg cactggcatc gggaagcgat tcagcaaagg
    60661 ggagatacgg tttgatatcc actaccggcg taccatcgac cagatccaga ctgccgagct
    60721 tcagaatcac gctatcttta tggcaaacaa cctctttcag ctctaccagc gacataccaa
    60781 tcgggttagg gcgaaaagtg gagcgcgtag cgaaaacacc cattctggca ttaccgccga
    60841 gacgaggcgg acgcacagtc ggacgccagc cgccttccat cgtttgatga aagacgaaaa
    60901 ggatccataa atggctgaat gcttccaggc cgcgaacggc gtcggcctgg ttgtagggag
    60961 caatgagatg tagttcgccg ttggcgcttt ttaccagacc tggctggcgc ggaacggcga
    61021 atttttcttt atagggcgag cgaataacgc ctatttgctc gaactggaaa ctgctcattt
    61081 cgccgtgatg ttaagcgcag aaccgataca tacagcctga cgatagcaac ctggcgtacc
    61141 gctggtgact tcgcagctat gcagtaatac cgcattggct ttcattttcg aggcgttgat
    61201 ttgcatccgc ttacgtgcgg ttggaatgct cggcggagag tcctgattag aggcctggca
    61261 agagtcgcca ctgacttcac cgagatcgcg gaacggtttg ccgactaatt cttctgcatt
    61321 ggtataaatt cggaccggcg tggcgcgcgg cgctttcggt tttgcaggct ccgctttcgg
    61381 ctggggtgca gtgctttgaa cgggttcgac aggggatctg cttaacatgg aacagccgct
    61441 tagcatgagt gctactaaac agatcggtaa agcacgcata gtatttcctc aatgtatgat
    61501 caaaacgtca atattgaatc aggcgcttgt aaaaatgaca agacgggcaa gcgcccgtcc
    61561 tgaatgatat tacaaattgt ggaaacagcc taaatattac cagcctttaa cagcgccgcc
    61621 gttaaacact ttgtttgctg cttcgtaaac ttcgtcagac tgataagcct gaacgaattt
    61681 cttcacgttt tcggcgtctt tgttgtcttc acgcgttacg atcaggttta cgtacgggga
    61741 ctctttatct tcgacaaaga taccgtcttt cgctggagtc aggccaatct ggctggcata
    61801 ggtggtattg ataactgcca gagcgatttg cgcgtcgtcc agagagcgcg gcagctgcgg
    61861 tgcttccagt tcaacaattt tcagattttt tgggttctca acaacatcaa gaacggtcgg
    61921 cagcaggcca acgccatctt tcagtttgat caagcccact ttttgcagca gcagcagtga
    61981 acgaccaagg ttagttgggt cgtttggcac ggcaacctgc gaaccatcct gcagttcatc
    62041 cagtgatttg attttcttgg agtaaccagc aatcggataa acaaaagtgt tgcctactgc
    62101 gaccagtttg tagccacgat ctttcagttg ctgatcaagg tacggtttat gctggaaggc
    62161 gttggcgtcg atatcgcctt tgctcaatgc ttcgtttggc agaacatagt cgttgaaggt
    62221 taccagctca acgtccaggc catatttgtc tttcgcaact ttctgcgcga cttctgcaac
    62281 ctgctgttcg gcaccaacaa tcacgccgac tttaatgtgg tttggatctt tttcatcctg
    62341 accgcagcct accagtgcca gtgatccaat cagggctccc actgccgcaa aggttttgaa
    62401 tttgaacgcc ataccttatt ccttcttcaa ttatttatgt tgtgttgaac gttacttgcg
    62461 agtgacagcc cggacgatgc ggtcgcctgc gaactgaatt aaataaacca gaatgaccag
    62521 caataccagt accgtattca tcaccgtcgc gttgtagccg atgtagccat actgatagcc
    62581 aatctgacct aaaccaccgg caccgactgc accacccatc gcggaataac caaccagggt
    62641 aatcagggtg atagttgccg cattcaccag acccggcaac gcttccggta acagcacttt
    62701 acggacgatc tgcattggcg tggcccccat tgcgcgggaa gcttcaatta acccggttgg
    62761 gatctccagc agagcgttct cgaccatacg ggcaataaac ggtgctgcac caacggttaa
    62821 cggaacaatc gctgcctgca atccaatcga tgtaccgaca ataacgcggg taaacggaat
    62881 catccatacc agcaagataa tgaacgggat ggaacggaaa atgttcacaa tcgcagaaat
    62941 ggtacgatac aacttcgcgt tagcaataat ttgccccgga cgcgtgacat aaagcagaac
    63001 gccaaccggc agaccaatca caaagccaaa aaaaccggat acgaaggtca ttgccagcgt
    63061 ttcccatacg ccacgaacca gcagccacat catcggctca gacataaccc agtacctcta
    63121 cttttacatg gtgttcctgc agccaggcaa tggcggcttg cgtatcttgt tgtgtgccgt
    63181 gcatttcagt cagcatgatg ccgaacttaa cgccaccggc gtaatccatc tgcgcgctaa
    63241 taatgttgtt gttgacgttg aaacgacgcg cggtttcaga aagcagtggg gcatcgaccg
    63301 attgaccggt aaactccaga cgcagcatcg gcacgcagtc agtaaatggc tccgcttgca
    63361 gacgttcctg gtaatcttcc gggatatcca gatgcagggt cgactgaata aacttctgcg
    63421 ccagcggcgt tttcggatgc gagaacactt cacttaccgt gtcctgctcg atcagttcgc
    63481 cattgctgat gaccgccacg caatcacaaa tgcgcttcac aacgtccatt tcgtgagtga
    63541 tcaacagaat cgtcaaaccc agacggcggt tgatgtcttt cagcagttcg agaatagaac
    63601 gtgtcgttgc cgggtccagc gcgctggtgg cttcatcaca cagcaatact ttgggattgc
    63661 tggctaatgc acgggcaatc gccacacgtt gtttctgccc accggaaaga ttcgacgggt
    63721 agctatcatg cttatcgcca agaccaacca atgacagcaa ttccgtcacg cgacgtttga
    63781 tctcgtcttt cggtgtgttg tccagctcca gcggcagagc cacgttgcca aaaacagtac
    63841 gcgaagagag caggttaaaa tgctggaaaa tcataccaat ctggcgacga gctttggtca
    63901 actcggattc tgacagcgtg gtcaattcct ggccatcgac cagcacgcta ccctcggttg
    63961 ggcgctccag caggtttaca caacgaataa gcgtactctt acccgcgcct gaggcaccga
    64021 taacgccata aatttgtcca gctggcacat gcaggctgac gttgttcaac gcctggatgg
    64081 tgcgggtgcc ctggtggaac actttggtga tattcgaaag ttttatcatt gattatttat
    64141 tatcgtcatt aagttagtcg tggcatttcg aatgcctgga acgggcatcg ccgtcaatga
    64201 aatggatgtt aaggcatcca gacgtctaaa tcaatcaggt ttatgcgaag agcgctttct
    64261 tgcaggtcga aacatgcgat actagcgtca catgccttat taaggagcta taaaaggtgg
    64321 cgaagagcgt acccgcaatt tttcttgacc gtgatggcac cattaatgtt gatcacggct
    64381 atgtccatga gatcgacaac tttgaattta tcgacggtgt tattgacgcc atgcgcgagc
    64441 taaaaaaaat gggctttgcg ctggtggtag taaccaacca gtctggcatt gctcgcggta
    64501 aatttactga agcacagttt gaaacgctga ccgagtggat ggactggtcg ctggcggacc
    64561 gcgatgtcga tctggatggc atctattatt gcccgcatca tccgcagggt agtgttgaag
    64621 agtttcgcca ggtctgcgac tgccgcaaac cacatccggg gatgtttttg tcagcacgcg
    64681 attatttgca tattgatatg gccgcttctt atatggtggg cgataaatta gaagatatgc
    64741 aggcagcggc tgcggcgagc gtgggaacaa aagtgctggt gcgtacgggt aaacctatta
    64801 cgcctgaagc agaaaacgcg gcggattggg tgttaaatag tctggcagac cttccgcaag
    64861 cgataaaaaa gcagcaaaaa ccggcgtgat gattaaaaga tgagcggttg aaataaaaat
    64921 gcatttttcc gcttgtcttc ctgaaacgac tccctataat gcgcctccat cgacacggcg
    64981 gatgtgaatc acttcacaca aacagccggt tcggttgaag agaaaaattc tgaaattcag
    65041 ggttgactct gaaagaggaa agcgtaatat acgccacctc gcgacagtga gctgaaagcc
    65101 gagtcgcaac tgctctttaa caatttatca gacaatctgt gtgggcactc gaagatacgg
    65161 attctt
//
LOCUS       DAZGJT010000014        63708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZGJT010000014 DAZGJT010000000
VERSION     DAZGJT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.014"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..209
                     /locus_tag="ACHKFK_000993"
     CDS             75..209
                     /locus_tag="ACHKFK_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9051393.1"
                     /translation="MIYYLGYDRFLLIFFTDYSHFMDRITLPSTDTEKGKGKWKIITA
                     "
     gene            188..292
                     /gene="speFL"
                     /locus_tag="ACHKFK_000994"
     CDS             188..292
                     /gene="speFL"
                     /locus_tag="ACHKFK_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO9051394.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            602..2800
                     /gene="speF"
                     /locus_tag="ACHKFK_000995"
     CDS             602..2800
                     /gene="speF"
                     /locus_tag="ACHKFK_000995"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO9051395.1"
                     /translation="MSELKIAVSRSCPDCFSTHRECVNIDKSNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLELNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFKFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     EADAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2797..4116
                     /gene="potE"
                     /locus_tag="ACHKFK_000996"
     CDS             2797..4116
                     /gene="potE"
                     /locus_tag="ACHKFK_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO9051396.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYTLYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4173..5813)
                     /gene="pgm"
                     /locus_tag="ACHKFK_000997"
     CDS             complement(4173..5813)
                     /gene="pgm"
                     /locus_tag="ACHKFK_000997"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO9051397.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5839..6384)
                     /gene="seqA"
                     /locus_tag="ACHKFK_000998"
     CDS             complement(5839..6384)
                     /gene="seqA"
                     /locus_tag="ACHKFK_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO9051398.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6569..7333
                     /gene="ybfF"
                     /locus_tag="ACHKFK_000999"
     CDS             6569..7333
                     /gene="ybfF"
                     /locus_tag="ACHKFK_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9051399.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPTLFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7473..7766
                     /gene="ybfE"
                     /locus_tag="ACHKFK_001000"
     CDS             7473..7766
                     /gene="ybfE"
                     /locus_tag="ACHKFK_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO9051400.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7906..8436
                     /gene="fldA"
                     /locus_tag="ACHKFK_001001"
     CDS             7906..8436
                     /gene="fldA"
                     /locus_tag="ACHKFK_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO9051401.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8646..8732
                     /gene="uof"
                     /locus_tag="ACHKFK_001002"
     CDS             8646..8732
                     /gene="uof"
                     /locus_tag="ACHKFK_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO9051402.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8725..9171
                     /gene="fur"
                     /locus_tag="ACHKFK_001003"
     CDS             8725..9171
                     /gene="fur"
                     /locus_tag="ACHKFK_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO9051403.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9255..9581)
                     /gene="chiQ"
                     /locus_tag="ACHKFK_001004"
     CDS             complement(9255..9581)
                     /gene="chiQ"
                     /locus_tag="ACHKFK_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO9051404.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9631..11037)
                     /gene="chiP"
                     /locus_tag="ACHKFK_001005"
     CDS             complement(9631..11037)
                     /gene="chiP"
                     /locus_tag="ACHKFK_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO9051405.1"
                     /translation="MRAFSGKRSTLALAIAGVTAMSGFMAIPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFILEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11478..13142)
                     /gene="glnS"
                     /locus_tag="ACHKFK_001006"
     CDS             complement(11478..13142)
                     /gene="glnS"
                     /locus_tag="ACHKFK_001006"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO9051406.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRTGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13362..14123)
                     /locus_tag="ACHKFK_001007"
     CDS             complement(13362..14123)
                     /locus_tag="ACHKFK_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9051407.1"
                     /translation="MKGYNRLEQIMDYLKSHNLVTVDELVAVTNASPATIRRDLIKLD
                     EQGVISRTHGGVTLNRFIPTQPTTHEKMQRSLAEKHAIASAAASMVKAGDSVVLDAGT
                     TMIELARQITHLPLRVITSDLHIALFLAEFKQIEVTIIGGRIDDSSQSCIGEHGRKLL
                     QNTWPDVAFLSCNSWDLEKGITAPTEEKAALKRDLIAHASRKILLADSSKYGSWSLFN
                     IAHLNELTEIITDSQLDGQTRRTLASLSTRLIIAD"
     gene            complement(14125..15111)
                     /locus_tag="ACHKFK_001008"
     CDS             complement(14125..15111)
                     /locus_tag="ACHKFK_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-threonate 4-phosphate dehydrogenase"
                     /protein_id="HFO9051408.1"
                     /translation="MNNIIAVTMGDPAGIGPEIIIKSLSEAGLAGAPVVVVGCVRTLR
                     RIMAMNITPQAELRVIQKVCDAHFAPGVINVMDEPLANPDALTPGVVQAAAGDLAYRC
                     IKRATALALSGEVKAIATAPLNKEALHLGGHNYPGHTELLAHLTGSKEYAMVLYTDKL
                     KVIHISTHISLRKFLDTLNGERVKTVIRVANHFLKRVGIERPRIAVAGVNPHAGEHGL
                     FGTEEIEIIAPAIEAMQAEDIDVTGPCPPDTVFMQCHEGLFDMVVAMYHDQGHIPLKL
                     LGFYDGVNITAGLPFIRTSADHGTAFDIAWTGKAKSESMAVSIQLAMQISRE"
     gene            complement(15104..16339)
                     /locus_tag="ACHKFK_001009"
     CDS             complement(15104..16339)
                     /locus_tag="ACHKFK_001009"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HFO9051409.1"
                     /translation="MEKGITKPSILVVADDFTGANDAGVSLAQVGHTVDVAFEMPYRG
                     DASVWVINSDSRAMDPKLAAMKITSLMSHLPLANNPPLVIKKIDSTLRGNIGAEIEAL
                     MKACGITGAVVAPAFPQAGRTTVAGECWVNGVRITETEFASDPKTPVLSARIADIIRL
                     QTAIPCQPVTVSQLSHLSYEQPWISVIDAQTDSDLDRIAAAVMQAKQPLLLVGSAGIC
                     DAVARRSAIMPPPTVLAIIGSMSEIAQRQIATLHSHPCITQIYVDVEHILAGNASDYD
                     ARIVQALQKGDHCIVHTCNDSVARHQIDTLCQRWQMSRAALGEKICRFLGELTRQVLL
                     RTMPDALYLSGGDVAMATASALGATGFRITGKVAQCVPYGHFLGGVWSRSVMTKAGGF
                     GDETTLHQVLNFIEEKCSE"
     gene            16509..17666
                     /locus_tag="ACHKFK_001010"
     CDS             16509..17666
                     /locus_tag="ACHKFK_001010"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HFO9051410.1"
                     /translation="MVIINSTILSGAGAIPSLTSLLPDIRKMLLVTDRNIAQLDGVQQ
                     IRALLEKHCPQVNVIDNVPAEPTHHDVRQLMDVLGDASFDVVVGIGGGSVLDVAKLLS
                     VLCHPQSPGLDALLAGEKPTQRVQSWLIPTTAGTGSEATPNAILAIPEQSTKVGIISQ
                     VLLPDYVALFPELTTSMPAHIAASTGIDALCHLLECFTATVANPVSDNAALTGLSKLF
                     RHIQPAVNDPQDLRAKLEMLWASYYGGVAITHAGTHLVHALSYPLGGKYHLPHGVANA
                     ILLAPCMAFVRPWAVEKFARVWDCIPDAETALSTEEKSHALVTWLQALVHQLKLPNNL
                     ADLGVPPEDIASLSEVALNVKRLMNNVPCQLDLQDVQAIYQTLFPQHQFKE"
     gene            17670..18557
                     /locus_tag="ACHKFK_001011"
     CDS             17670..18557
                     /locus_tag="ACHKFK_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HFO9051411.1"
                     /translation="MPKTIEGVLTAIVTPFTATGSLNIPALKVQVNRQLEAGNAIFCG
                     GTNGEFFVLNEQEKLSVTQTCVDEVAGRAPVVAHIGEISTRETIRLGQQIEKLGVDAV
                     SVIAPYFVPLKQEELIAHYSAIADALSIPMFLYNIPARTGNTIQPETARALASHPNII
                     GIKDSAGSDESLKGFLDAVRDIDGFNVLNGPDSLIHKGFVEGCSTCISGLANIAPREI
                     NAIWSRFHAGDIEGSYEAQESVTGLRTDLYKVAFSPAAVKKALQLMGHDVGDSRYAVN
                     FTPEQISEIQQIIRHYNIN"
     gene            18617..19375
                     /locus_tag="ACHKFK_001012"
     CDS             18617..19375
                     /locus_tag="ACHKFK_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9051412.1"
                     /translation="MFLWFMQPDIWLTLITLTFLEIILGVDNIIFLSLVVAKLPLSQQ
                     NMARKLGLSCAMIMRILLLISIAWLSHITQPLFTVLTLEISFRTLILLGGGLFLIYKA
                     IQEIREELSPAQEDEHGSGSRQLSLCGAIVQIMLLDIVFSLDSVITAVGLSQHIFIMI
                     AAVMIAVGVMMFAAKTIGDFVNATPSIKILALTFLLFVGVLLVADSLNIHIAKEYLYF
                     AIFFSLSVETLNIIRERKNKKVSTLIETHESHSH"
     gene            19411..20580
                     /locus_tag="ACHKFK_001013"
     CDS             19411..20580
                     /locus_tag="ACHKFK_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000109428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HFO9051413.1"
                     /translation="MSVALIRDGVIRPSENDSHVLTAMLPSSCPQNHAANILPLPDGA
                     LMCVWFAGTQEGIADISVWGSRLPAGGMQWSDAVKLSHDDTRSEQNPVLFLAPDNVLW
                     LLWTAQISGNQDTAIVRYRKSDDLGQTWGEIATLLDKPGTFIRQPITVLDNGNWLLPV
                     FYCRTQPGEKWVGNDDISAVKISADGGHSWRDVEVPQSLGCVHMNITMLHNGTLVAFF
                     RSRWADNIYISHSADNGESWSVPQATELPNNNSSIQVTTLASGELALVYNAMSAAGAV
                     EHRASLYDEIDDGDDSRKEPTAVGRSAFWGAPRAPMTVAISADGGKSWPWRRNLDEGD
                     GYCMTNNSLEKLNREFSYPSIKQSPDGTLHIAYTWWRQAIKYVRISPEWVKGQAS"
     gene            20577..21047
                     /locus_tag="ACHKFK_001014"
     CDS             20577..21047
                     /locus_tag="ACHKFK_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9051414.1"
                     /translation="MIIGNLNHLSLAGLPAWVRNILLRPECSLSALSTREDGRWQPEG
                     CRWFCTLGTSDTQPAELRHTEYHHLWADIQVVITGCEGINAGTRPIARENDEERKPDL
                     FIAPSPENSVAITLHAGDFAVFMPEEPHQALCAIGTATSIRKAVFKVPRDMLEA"
     gene            21061..22341
                     /locus_tag="ACHKFK_001015"
     CDS             21061..22341
                     /locus_tag="ACHKFK_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO9051415.1"
                     /translation="MTMEMILALGILVLMIVLIMSDKMPFGAPPLLACLLLVVSGLST
                     VQQAFAGFVNPSVVMIAGFMVVMAALQKTRLISNVKSAMISLVNKGSYRSYGLLLVIV
                     MLGASLAGTGATGYYVLILSLVSTIPYSKKLPTSKLMMPLGFATNHPLLPINLALLFG
                     VTATVLETAGFHQEISMGRFALVNLIMSAAFLAWSLIAYRFLPDHPIADASEDALAAR
                     EETFNALPAWKEYCTIAAFAVSVIGMMLMNILGNIAFVIPGLAGAFVLMIDVLDFKEV
                     RDHMGAPVILMMAGVIGVADALAGTGFTAMVGDAVAGVLGSGVSPFVFIVAFALLTST
                     CATFTGSNMGSVYIFAPIAIAACTSLGLNPTAAAIAVVISGWNGGYMPIDGMPAMILG
                     MGKYKLPEFWVFSVPMYLIRILALCAGAVFIFPM"
     gene            complement(22430..24376)
                     /gene="nagE"
                     /locus_tag="ACHKFK_001016"
     CDS             complement(22430..24376)
                     /gene="nagE"
                     /locus_tag="ACHKFK_001016"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO9051416.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            24709..25509
                     /gene="nagB"
                     /locus_tag="ACHKFK_001017"
     CDS             24709..25509
                     /gene="nagB"
                     /locus_tag="ACHKFK_001017"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO9051417.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25569..26717
                     /gene="nagA"
                     /locus_tag="ACHKFK_001018"
     CDS             25569..26717
                     /gene="nagA"
                     /locus_tag="ACHKFK_001018"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO9051418.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAISVEKRLGTLAAGKVANLTAFTPDFKITRTIVNGNEVVTQ"
     gene            26726..27946
                     /gene="nagC"
                     /locus_tag="ACHKFK_001019"
                     /gene_synonym="nagR"
     CDS             26726..27946
                     /gene="nagC"
                     /locus_tag="ACHKFK_001019"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO9051419.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27994..28746
                     /gene="nagD"
                     /locus_tag="ACHKFK_001020"
     CDS             27994..28746
                     /gene="nagD"
                     /locus_tag="ACHKFK_001020"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO9051420.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTYGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            29002..30666
                     /gene="asnB"
                     /locus_tag="ACHKFK_001021"
     CDS             29002..30666
                     /gene="asnB"
                     /locus_tag="ACHKFK_001021"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO9051421.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILTLYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDYDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFEAYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31047..31123
                     /locus_tag="ACHKFK_001022"
     tRNA            31047..31123
                     /locus_tag="ACHKFK_001022"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31081..31083,aa:Met,seq:cat)
     gene            31134..31218
                     /locus_tag="ACHKFK_001023"
     tRNA            31134..31218
                     /locus_tag="ACHKFK_001023"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31168..31170,aa:Leu,seq:tag)
     gene            31242..31316
                     /locus_tag="ACHKFK_001024"
     tRNA            31242..31316
                     /locus_tag="ACHKFK_001024"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31274..31276,aa:Gln,seq:ttg)
     gene            31351..31425
                     /locus_tag="ACHKFK_001025"
     tRNA            31351..31425
                     /locus_tag="ACHKFK_001025"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31383..31385,aa:Gln,seq:ttg)
     gene            31441..31517
                     /locus_tag="ACHKFK_001026"
     tRNA            31441..31517
                     /locus_tag="ACHKFK_001026"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31475..31477,aa:Met,seq:cat)
     gene            31566..31640
                     /locus_tag="ACHKFK_001027"
     tRNA            31566..31640
                     /locus_tag="ACHKFK_001027"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31598..31600,aa:Gln,seq:ctg)
     gene            31678..31752
                     /locus_tag="ACHKFK_001028"
     tRNA            31678..31752
                     /locus_tag="ACHKFK_001028"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31710..31712,aa:Gln,seq:ctg)
     gene            complement(31906..33081)
                     /gene="ubiF"
                     /locus_tag="ACHKFK_001029"
     CDS             complement(31906..33081)
                     /gene="ubiF"
                     /locus_tag="ACHKFK_001029"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO9051422.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVMVIEHAQPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRHNDLQELELKGGETILAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLIRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFMMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRKALKYALGL"
     gene            33227..34651
                     /gene="miaB"
                     /locus_tag="ACHKFK_001030"
     CDS             33227..34651
                     /gene="miaB"
                     /locus_tag="ACHKFK_001030"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO9051423.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGNRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTDDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLDTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34804..35844
                     /gene="ybeZ"
                     /locus_tag="ACHKFK_001031"
     CDS             34804..35844
                     /gene="ybeZ"
                     /locus_tag="ACHKFK_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO9051424.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35841..36308
                     /gene="ybeY"
                     /locus_tag="ACHKFK_001032"
     CDS             35841..36308
                     /gene="ybeY"
                     /locus_tag="ACHKFK_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO9051425.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQSK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEAIETEIMLALGYEDPYIAEKE"
     gene            36398..37276
                     /gene="corC"
                     /locus_tag="ACHKFK_001033"
     CDS             36398..37276
                     /gene="corC"
                     /locus_tag="ACHKFK_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO9051426.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37301..38842
                     /gene="lnt"
                     /locus_tag="ACHKFK_001034"
     CDS             37301..38842
                     /gene="lnt"
                     /locus_tag="ACHKFK_001034"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO9051427.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVTVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSLNSIQLTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRKR"
     gene            complement(38974..40851)
                     /locus_tag="ACHKFK_001035"
                     /pseudo
     CDS             complement(38974..40851)
                     /locus_tag="ACHKFK_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
     gene            41305..42213
                     /gene="gltI"
                     /locus_tag="ACHKFK_001036"
     CDS             41305..42213
                     /gene="gltI"
                     /locus_tag="ACHKFK_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO9051428.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNAERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            42383..43123
                     /gene="gltJ"
                     /locus_tag="ACHKFK_001037"
     CDS             42383..43123
                     /gene="gltJ"
                     /locus_tag="ACHKFK_001037"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO9051429.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            43123..43797
                     /gene="gltK"
                     /locus_tag="ACHKFK_001038"
     CDS             43123..43797
                     /gene="gltK"
                     /locus_tag="ACHKFK_001038"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO9051430.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            43797..44522
                     /gene="gltL"
                     /locus_tag="ACHKFK_001039"
     CDS             43797..44522
                     /gene="gltL"
                     /locus_tag="ACHKFK_001039"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO9051431.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRNKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            44640..45575
                     /gene="rihA"
                     /locus_tag="ACHKFK_001040"
     CDS             44640..45575
                     /gene="rihA"
                     /locus_tag="ACHKFK_001040"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO9051432.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVASGAVKPLMRNLIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESEEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(45620..46102)
                     /gene="ybeL"
                     /locus_tag="ACHKFK_001041"
     CDS             complement(45620..46102)
                     /gene="ybeL"
                     /locus_tag="ACHKFK_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO9051433.1"
                     /translation="MNKVAQHYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            46337..48919
                     /gene="leuS"
                     /locus_tag="ACHKFK_001042"
     CDS             46337..48919
                     /gene="leuS"
                     /locus_tag="ACHKFK_001042"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO9051434.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSKAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            48934..49515
                     /gene="lptE"
                     /locus_tag="ACHKFK_001043"
     CDS             48934..49515
                     /gene="lptE"
                     /locus_tag="ACHKFK_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO9051435.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            49515..50546
                     /gene="holA"
                     /locus_tag="ACHKFK_001044"
     CDS             49515..50546
                     /gene="holA"
                     /locus_tag="ACHKFK_001044"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO9051436.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQQQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            50548..51189
                     /gene="nadD"
                     /locus_tag="ACHKFK_001045"
     CDS             50548..51189
                     /gene="nadD"
                     /locus_tag="ACHKFK_001045"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO9051437.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            51213..51824
                     /gene="cobC"
                     /locus_tag="ACHKFK_001046"
     CDS             51213..51824
                     /gene="cobC"
                     /locus_tag="ACHKFK_001046"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO9051438.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHD
                     VSFDLVLCSELERAQHTARLVLSDRQHPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILVVSHQGVLSLLITRL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            52084..52401
                     /gene="rsfS"
                     /locus_tag="ACHKFK_001047"
     CDS             52084..52401
                     /gene="rsfS"
                     /locus_tag="ACHKFK_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO9051439.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            52405..52872
                     /gene="rlmH"
                     /locus_tag="ACHKFK_001048"
     CDS             52405..52872
                     /gene="rlmH"
                     /locus_tag="ACHKFK_001048"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO9051440.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDIPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            52903..54804
                     /gene="mrdA"
                     /locus_tag="ACHKFK_001049"
     CDS             52903..54804
                     /gene="mrdA"
                     /locus_tag="ACHKFK_001049"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO9051441.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            54807..55919
                     /gene="mrdB"
                     /locus_tag="ACHKFK_001050"
                     /gene_synonym="rodA"
     CDS             54807..55919
                     /gene="mrdB"
                     /locus_tag="ACHKFK_001050"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO9051442.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTAIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            55930..57018
                     /gene="rlpA"
                     /locus_tag="ACHKFK_001051"
     CDS             55930..57018
                     /gene="rlpA"
                     /locus_tag="ACHKFK_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO9051443.1"
                     /translation="MRKQWLGICIAAGMLTACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVPVPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            57158..58369
                     /gene="dacA"
                     /locus_tag="ACHKFK_001052"
     CDS             57158..58369
                     /gene="dacA"
                     /locus_tag="ACHKFK_001052"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO9051444.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            58479..58742
                     /gene="ybeD"
                     /locus_tag="ACHKFK_001053"
     CDS             58479..58742
                     /gene="ybeD"
                     /locus_tag="ACHKFK_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO9051445.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            58843..59484
                     /gene="lipB"
                     /locus_tag="ACHKFK_001054"
     CDS             58843..59484
                     /gene="lipB"
                     /locus_tag="ACHKFK_001054"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO9051446.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPLIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            59744..60064
                     /locus_tag="ACHKFK_001055"
                     /pseudo
     CDS             59744..60064
                     /locus_tag="ACHKFK_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            60305..61270
                     /gene="lipA"
                     /locus_tag="ACHKFK_001056"
     CDS             60305..61270
                     /gene="lipA"
                     /locus_tag="ACHKFK_001056"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO9051447.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(61370..61573)
                     /gene="tatE"
                     /locus_tag="ACHKFK_001057"
     CDS             complement(61370..61573)
                     /gene="tatE"
                     /locus_tag="ACHKFK_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO9051448.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(61702..62490)
                     /locus_tag="ACHKFK_001058"
     CDS             complement(61702..62490)
                     /locus_tag="ACHKFK_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO9051449.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENEVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLDRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            62583..62966
                     /gene="crcB"
                     /locus_tag="ACHKFK_001059"
     CDS             62583..62966
                     /gene="crcB"
                     /locus_tag="ACHKFK_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO9051450.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTTH"
     gene            complement(63020..63229)
                     /gene="cspE"
                     /locus_tag="ACHKFK_001060"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(63020..63229)
                     /gene="cspE"
                     /locus_tag="ACHKFK_001060"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9051451.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(63404..>63708)
                     /gene="pagP"
                     /locus_tag="ACHKFK_001061"
     CDS             complement(63404..>63708)
                     /gene="pagP"
                     /locus_tag="ACHKFK_001061"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO9051452.1"
                     /translation="EKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGL
                     GFTAGVTARDNWNYIPLPVLLPLASVGYGPATFQMTYIPGTYNNGNVYFAWMLFQF"
BASE COUNT        15580 a        16434 c        16083 g        15611 t
ORIGIN      
        1 aatgaaaata caacccacaa aattgcaaca tttaaacaac aaaacagcat ctaatatggc
       61 gctattcatc attaatgatt tattatttgg gatatgatcg ttttttgttg atcttcttca
      121 cagattatag ccatttcatg gatagaataa ccctaccttc aactgacaca gaaaaaggta
      181 aaggtaaatg gaaaataata accgcttaat gcctcatata aggcggacaa cccatatcat
      241 gaagtttgcc catcgtaata gttttgactt tcatttcttt aatgcccgct agtcttctga
      301 ctaaagggca ccccagcgta caggtctccc tgactttaag cattacaggt taatacctgt
      361 atccctcggt gctcatatac tgctaaccct tttaaactct aaataattcg agtcgcagca
      421 taggcaactt gaagtatgac gagtatagcc agttaccggg ctggtctggg ttattgcatc
      481 tgcaaaaagc aaactactga tttatttatc agcggtgggg ttttgctttt ttccggcgtg
      541 atcaatttct tctttgagaa attgaggacc tgctattacc tgaaataaag agatgaacaa
      601 aatgtcagaa ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg
      661 tgaatgcgtg aatattgaca aaagtaatta tattgacgtt gccgccatta ttttatcagt
      721 caatgatgtt gaacgtggaa aactcgatga aatagacgct actggctatg gcattcctgt
      781 ttttattgca acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg
      841 tgtcttcgag cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc
      901 cagccattat gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa
      961 tcagggcaat agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgcca
     1021 tccggcaggc aatcagttcg tggaatattt tggcgaaatg ctcttccgct ccgacttgtg
     1081 taatgccgac gttgcaatgg gcgatctact gattcatgaa ggtgcgccat gcattgcaca
     1141 gcaacatgcg gctaaagtgt ttaatgccga taaaacgtac tttgttttaa acggcacctc
     1201 ctcctccaac aaagtggttt taaacgccct gctgacaccg ggtgatctgg tgctgtttga
     1261 ccgcaataac cataagtcta accatcacgg ggccttgcta caggctggtg caacaccggt
     1321 ttatctggaa acagcacgta acccgtatgg ttttatcggt ggcattgatg cgcactgctt
     1381 cgaagaaagt tatctgcgtg aactgattac agaagttgca ccgcaacggg caaaagaagc
     1441 gcgtccgttc cgcctcgctg tgattcagtt aggcacctac gacggtacta tttataacgc
     1501 ccgccaggtg gtggataaaa tcggtcatct gtgtgactac atcctgtttg actcagcatg
     1561 ggtcggctat gaacagttta ttccgatgat ggcggactgt tcgccgctgc tgctggagct
     1621 taatgagaac gatccgggta ttctggttac gcaatctgtg cataaacaac aggctggttt
     1681 ttctcagact tcacaaattc ataaaaaaga cagccatatc aaagggcaac agcgttatgt
     1741 accgcacaaa cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc
     1801 gctgtttgcc gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat
     1861 gtggatggat tgtgtggtta acggtattaa tgcccgcaaa ttgatcctcg ataactgcca
     1921 gcacattcgc ccgttcgttc ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac
     1981 agcgcaaatt gcggttgatc tgcgcttctt taaatttgta cccggtgagc actggcattc
     2041 ttttgaaggc tacgcagaga accaatactt tgtcgatccg tgcaaactgt tgctgacaac
     2101 tccaggtatt gatgcacgta acggcgaata tgaagcgttc ggtgtacccg caacgattct
     2161 tgctaacttc ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta actccatcct
     2221 cttcctgctg actccggcag aagatatggc caaactgcag caacttgttg ccctgctggt
     2281 acgcttcgaa aaactactgg aagctgatgc gcctctggcg gaagtactgc cttccatcta
     2341 caaacagcat gaagagcgct acgccggtta tacccttcgt cagttgtgcc aggaaatgca
     2401 tgatttgtat gcccgccaca acgtgaaaca actgcaaaaa gagatgttcc gtaaggaaca
     2461 cttcccacgc gtcagcatga atccgcaaga agccaactac gcctacttac gcggtgaagt
     2521 ggagctggtt cgtctgccgg atgcagaagg ccgtatcgct gccgaaggtg cgcttcctta
     2581 tcctccgggt gtgctgtgtg ttgttcctgg tgaaatctgg ggtggtgctg ttctgcgtta
     2641 cttcagcgct ctggaagaag gcatcaacct gctgccaggt tttgcaccgg agctgcaggg
     2701 tgtctatatc gaagaacatg atggtcgtaa gcaagtttgg tgctatgtca tcaagcctcg
     2761 tgatgcgcaa agcaccctgt tgaaagggga aaaattatga gtcaggctaa atcgaacaaa
     2821 atgggtgtcg ttcagttaac catactgacg atggtcaaca tgatgggctc cggtatcatc
     2881 atgctgccga caaagcttgc cgaagtcggg acaatctcaa ttatctcctg gctggtgaca
     2941 gccgtaggct caatggcact ggcatgggca ttcgcaaaat gcggtatgtt cagccgtaaa
     3001 tcaggaggta tgggcggtta tgccgagtat gctttcggta aatccggtaa ctttatggcg
     3061 aactatacct acggcgtctc gctgctgatt gctaacgtcg cgattgctat ttcggcggtt
     3121 ggttacggca ccgaactgct cggcgcgagt ttgtcgccag tgcagattgg tcttgcgacc
     3181 atcggggtgc tgtggatttg taccgtggct aactttggtg gtgcgcgcat taccgggcaa
     3241 atcagtagca ttaccgtgtg gggggtcatt attccggtgg taggcctgtg cattatcggc
     3301 tggttctggt ttagcccgac actttacgtt gattcctgga atccgcatca tgcaccgttc
     3361 ttcagtgcgg taggttcttc catcgctatg acgctgtggg cttttcttgg tctggagtct
     3421 gcgtgtgcga atactgatgt agtggaaaac ccggaacgta atgtgccaat cgcggtactc
     3481 ggcggtacgt taggtgcggc ggtgatttat atcgtctcca ccaacgtgat tgccgggatt
     3541 gtgccaaata tggagctggc aaattcaacg gcaccatttg gtctggcctt cgcgcagatg
     3601 ttcacgccgg aagtgggtaa agtcattatg gcgctgatgg tgatgtcctg ctgcggttcg
     3661 ctgcttggct ggcagttcac cattgcccag gtgtttaaat cttcatctga tgaaggctac
     3721 ttccctaaaa ttttctcccg tgtaaccaaa gtggatgcac cggtgcaggg aatgttgacc
     3781 attgtgatta tccagagcgg gttggcgctg atgacgatta gcccgtcgct aaacagtcag
     3841 ttcaacgtgc tggttaacct ggccgtggtg accaatatca ttccgtatat tctgtcaatg
     3901 gcggcattag tcattattca gaaggtcgct aatgtgcccc cctcaaaagc gaaagttgca
     3961 aactttgttg cttttgttgg cgcgatgtac agcttttata cgctgtactc atccggggaa
     4021 gaagccatgc tatacggttc catcgtgacc ttcctcggtt ggacactgta tggtctggtc
     4081 tcaccacgct ttgaactgaa aaataaacac ggttaattaa gcaaagcaaa aaagggcgat
     4141 cttccgaccg ccctttttct tatttgatat gtttacgcgt ttttcagaac ttcgctaaca
     4201 atctcaaccg cttctttctc aatctgcttg cgatgttctt caccaagaaa gctttcgcag
     4261 tagatcttat atgcgtcttc cgtgcctgac ggacgcgcgg cgaaccagcc gttgtcagtc
     4321 atcactttca gaccgccgat agaagcgcca ttacccggtg ccgccgtcag acgcgcggtg
     4381 atcgggtcac ctgccagggt gctggcgctc accatttccg gagacagctt agacaacgcc
     4441 gctttttgtg cggaagtcgc tgctgcctgc aaacggttat agctcggcgc accaaagcgt
     4501 tttgccagtt cgttatagtg ttcctgcggg ttcttaccgg tgacagcggt gatttccgcc
     4561 gccagcagac acataatgat accgtcttta tcggtggacc acggcgtgcc gtcgaaacgc
     4621 aggaaggaag cgcctgcact ctcttcgccg ccaaagccga agctgccatc gaacaaacca
     4681 tcaacaaacc atttgaaacc taccggtact tctaccagct tacggcccaa atcattgacc
     4741 acacggtcga tcatcgcaga tgaaaccagc gttttaccga cagcaacatc tttgccccac
     4801 tgcggacgat gctggaacag gtagttgatc gccactgcca ggtagtggtt cggattcatc
     4861 aaacctgccg gagtgacgat accgtgacgg tcataatccg ggtcgttagc aaacgccaga
     4921 tcgaacttat cacgcagtgc cagcaggccc gccatcgcac actcggagga gcagtccata
     4981 cggatcgcac cgtctttatc gaggtgcata aagcggaagg tttgatcgac ctgatcgtta
     5041 acgatggtca ggttgaggtt gtaatactcg ccaatgcgct tccagtattc gataccggaa
     5101 ccacccagcg gatcaacgcc cagcgtcagg cccgctttct gaatcgccgc catatcaacg
     5161 atatcggcca acccttccac aaacggctgc accagatcct gctctttcac atgaccggat
     5221 gccatcgctt cgtcgaggga gatacgcttc acgcctttca ggccatcagc cagcagtgcg
     5281 ttggctctgt cttccaccac tttagtgacg ttggtatcag ccgggccacc atttggcgga
     5341 ttgtacttga taccaccatc ttccggcggg ttatgggacg gtgtaatcac gataccgtct
     5401 gccagcgggc cacctttttt attgtgaacc agaatggcat tggaaatggc aggtgtcgga
     5461 gtgaagccat tgttttcctg cacaatgaca tcaacgccgt tcgctgccag cacttccagt
     5521 acggaaatga acgcaggctc ggacagggcg tgagtatctt tacccacata gcaagggcca
     5581 gtgatgccgt ttttcgcacg ttcttcagca attgcctgag cgatcgccag aatgtgcggc
     5641 tcgttaaagc tgtggcgcgc tgcactgcca cggtgaccgg aagtaccgaa tttcaccgcg
     5701 tgctccgcat tccctgcttc tggtttcagt acataatatt gcgccgtcag ttgggcgacg
     5761 ttaatcaaat cactctgttg tgcaggttgg cctgcacgat tgtggattgc cattgctttg
     5821 tcctttgtct gcaacgtttt agatagttcc gcaaaccttc tcaatcaatt ccgccgggaa
     5881 ttgcatcgac tgcatgatgt gttcgatcat gctgcattta cggccggtgt tggtgttggt
     5941 gatcacccaa tacggcgtac caggcacatg tttcggcttg gtctgattac catttttcag
     6001 cagcgtttgt tcatctgccg caaagtaaac gcgtgtacga ccgtgcaacg attccgttgc
     6061 ttcggcaaac gcctgggcgt caagagaata tagtgtagac aacagcagca taaagcgatt
     6121 gaccgctcgc ttttgctctg cgtattcgtc cgaaagcaga agttcacgca ttgcgcgaac
     6181 cttgtcttta atcgttttga ccggcttcgc ttcgacgata gcaggtgacg caacgcgaac
     6241 ctctttcgtc accggagcag caggctgtga tgcggcggaa aatttcaaca tacgccgtaa
     6301 aatgtcggat gcgctctcgc cgatatgctt agtgtggctg gcaatatagc tgtagagttc
     6361 atcatcaact tcaatcgttt tcatcttaat ccagtgcagt gtcttagctg aatacaagtc
     6421 gccaggagta tatgaataaa tcccggcagc ggatagcgtc aagcctggct tgcagaaaaa
     6481 ctcggaataa accttatttg ttgcagccct gtggcacaat ggtcaacata ataccctaac
     6541 ccgacaacgc gaaaaaaaga actttgccat gaaattgaat atccgcgcgc aaactgcaca
     6601 aaaccagcac aataattctc ccatcgttct tgtccatggt ctgtttggca gcctcgacaa
     6661 ccttggcgta ctggctcgcg atctggtaaa cgatcacaat atcatccagg ttgatatgcg
     6721 taaccacggt ctttcaccga gagatccggt aatgaattac ccggcgatgg cgcaggatct
     6781 tgttgatacc ctggatgcac agcagatcga caaagcaaca tttatcggtc actccatggg
     6841 cggaaaagcg gtaatggctc ttactgcact agcccccgat cgcattgaca agctggtagc
     6901 gattgatatt gcgccggtcg attatcacgt tcgccgtcat gatgagattt tcgcggccat
     6961 caacgcagtc agtgaatcag acgcacaaac acgccagcaa gcagcggcaa taatgcgcca
     7021 gcatcttaat gaagaagggg tgattcagtt tctgttgaaa tcttttgttg acggggagtg
     7081 gcgctttaac gtgccagtat tgtgggatca gtatccgcat attgtgggtt gggagaaaat
     7141 cccggcatgg gatcatccta ccctgtttat ccctggcggc aattctccgt atgttagcga
     7201 gcagtaccgt gatgatttac tggcacaatt tccacaggct cgggcgcatg tgattgcagg
     7261 cgcgggtcac tgggtccatg ctgaaaaacc ggatgcggta ttacgcgcta tccgtcgcta
     7321 tctcaatgat taacgcaact gattaaaaac ccagcgtccg cgtcgctttc cgcgcgtgga
     7381 cgttggcgtg actcatcgcg ctgatgtatt atggcgcgct atccatccgg gcaggcgcct
     7441 ggctgattgt ttcccccgaa gtcaccaaga tcatggccaa agaacaaacg gaccgtacga
     7501 cattagatct gttcgcgcac gagcgtcgac cgggacgacc gaaaactaat ccgctttcgc
     7561 gcgatgaaca gctgcgtatt aataaacgca accaactaaa acgcgacaaa gtacgtggcc
     7621 ttaagcgtgt cgaactgaag ctgaacgcgg aagctgtcga ggcactgaac gagctggcgg
     7681 aatcgcgcaa tatgagccgt agcgagctga tcgaagagat gttaatgcag caactggcgg
     7741 cgctgcgtag tcagggcatc gtttaaattt ccactttcat gtagcacagt gtgcagtcct
     7801 gctcgtttgc tgattccgca agactgcctg ttctgctatg attgccttta tccgtgggca
     7861 attttccacc cccatttcaa taagtttcaa gaggttattt cactcatggc tatcactggc
     7921 atctttttcg gcagcgacac cggtaatacc gaaaatatcg caaaaatgat tcaaaaacag
     7981 cttggtaaag acgttgccga tgtccatgac attgcaaaaa gcagcaaaga agatctggaa
     8041 gcatatgaca ttctgctgct gggcatccca acctggtatt acggcgaagc gcagtgtgac
     8101 tgggatgact tcttcccgac tctcgaagag attgatttta atggtaaact ggttgcgctg
     8161 tttggttgtg gcgaccagga agattacgca gaatacttct gcgatgcatt gggcaccatc
     8221 cgcgacatca ttgaaccgcg cggcgcaact atcgttggtc actggccaac cgcgggctac
     8281 cacttcgaag cgtcaaaagg tctggctgat gatgaccact ttgtcggtct ggctatcgac
     8341 gaagatcgtc agccggaact gactgctgaa cgtgtagaaa aatgggttaa acaaatttct
     8401 gaagagctgc atctcgacga aattctcaat gcctgatgtg atgcggcgta gactcatgtc
     8461 tacgccgtat taatagataa tgccaatcaa aataattgct acaaatttgt aacttttgct
     8521 gttgtacctg tacaatgtcc cggtgttcaa gtggccttgc cgttgtaaat gtaagctgtg
     8581 ccacgttttt attaacaata tttgccaggg acttgtggtt ttcatttagg cgtggcaatt
     8641 ctataatgat acgcattatc tcaagagcaa attctgtcac ttcttctaat gaagtgaacc
     8701 gcttagtaac aggacagatt ccgcatgact gataacaata ccgccctaaa gaaagctggc
     8761 ctgaaagtaa cgcttcctcg cttgaaaatc ctggaagttc ttcaggagcc ggacaaccat
     8821 cacgtcagtg cggaagattt atacaaacgt ctgatcgata tgggtgaaga aattggtctg
     8881 gctacggtat atcgcgtact gaaccagttt gacgacgctg gtatcgtcac ccgccacaat
     8941 tttgaaggcg gtaaatccgt atttgaacta acacagcaac atcaccacga tcacctgatc
     9001 tgcctcgact gcggtaaggt tatcgaattt agtgatgatt ccatcgaagc tcgtcagcgt
     9061 gaaatcgccg caaaacatgg cattcgcctg actaaccaca gtctctatct ttacggtcac
     9121 tgtgccgaag gcgattgccg cgaagatgag cacgcacacg aaggcaaata agccagcctg
     9181 aacgagaaaa gccaacctgc aggttggctt ttttatacaa gggaaataag aaaagcgaga
     9241 gttacagctc tcacttattt gttattactg cgaatttcct gccagacctt atcacaatcg
     9301 gctttcaccg cctggtcatt accggtttgc gttgcgcgca gacactgctg ataatccagt
     9361 acgcgtacac tttcctggtc agcaaactgc tgatgttgtt tctctttctt cagcacgttt
     9421 aacacgctct ggcaggcttc aattttttcc ggcgaacctt gcgcggtgtt gatacaggcg
     9481 ctgtaagcct ctttcagacg actgtcttcc tgcggtgcgg ttgattgcgc acaagctacc
     9541 agccccgatg ccatcatggc gatgagaatc agttttttca tagcaaattc ctcaaacgtg
     9601 cggcaggcac aaaacctgcc gcgtcgggca tcagaagatg gtgaatggtg cgattaccat
     9661 gaatttcacg tcgcgctcat cctggaaaat gttgccgtaa ccaccgcccc agcttgggat
     9721 gtcggagtgg ttgtcatatt cggtgaaatg cagtttgaac atcgtgccct tggcacgacc
     9781 gtcctgaatg gtgtaaaccg catccaggct gtaggcagac tcttcaatag tccggttctt
     9841 gtcgtagtac gcatccggat tgctctgcca ggtcgcaggt ttagcatccc atgcgtaaac
     9901 gtaggaagcg ccgatggcga agcctggaag attccagttt ttcaggtcat acatcgcacc
     9961 gaagaagacc gctttttcgc cgttggcgtt gaagtcggaa cggttatccc accagatatc
    10021 caggcgaccg ttcgaggaag cgtaggttgg agtcatacgt tgcaggaagt atccttgctg
    10081 accgtcagcc ttaacccagg taccttcgag gcgcaaatcc actacgtcag ccgcccggta
    10141 accaaaggtc aacgcctgca gccaggcggt gccgtcataa agatcgttga cgctgcgatc
    10201 gtcaacttta tcgcgcgtac catagaactg gtagctggtg gttaacgggc taccggcgat
    10261 atcaaatttg tagctggctt tggcaaaata ttgatcgata tacccttcgg cctgaccaaa
    10321 cgccgcttcc agtatgaagt tatttttgaa gtcgtacttc gccccaaggg agtgtagata
    10381 atcaactttg gtggttttat cgttctgata aaactcatcc atttccagat gccacggtgc
    10441 tttgtattcg ttggtccaca tgtaggagaa actcaacgca ccagcatcgc cgtaatcaaa
    10501 attcgccccg gcctccgccc cctgataagt acccggcata aagctccagt gtggcgctaa
    10561 cagcgtctga ccggttggct gaatataacc tgccctcgcc caaaccggac cgtatttaaa
    10621 tttggccgct gctttataca ggcttatacc gcttttatcg ccggaccagt cttcgtcata
    10681 ggctttatta ctttttgaaa acgcgatttc gttcgggtga gagctgtcgc cgttttccgc
    10741 catttcaatc gccgtaaaca cggcaatatc aaggccgaac atatcagcag cataaccaga
    10801 ctgaaaatcg aggttggcgt tccaggtgga gtgagaaagg ttggttttgt atttatcgcc
    10861 gtcggtaaca tctttacggt cgcgttcacg ctgccagtaa tagataccgc cggttaaggt
    10921 tgaatcgtcg atgaatcctt cggcgcgagc ctccggaatt gccataaagc ccgacatagc
    10981 tgtaacaccg gcgatagcca gcgccagcgt actacgtttg ccactaaacg cacgcatggg
    11041 ttaatcctct ttgacgtata aattgctgca ccaaaggtga acagcgaaaa aaataaaaat
    11101 taaaaaagta gcgttgagaa aagtctcgct gactacagga acagactatt tttcgcgacg
    11161 cgaattatca atctctttct gtaaccagaa tgtaagaaca tgagcaaccg cacatatttt
    11221 gatgattttt gttacaaggc ttaagtttgc agttgaaagg cttgcagaag aataaaaacg
    11281 tgaaattcca gcaagatgaa agcggttaca ttttatttaa ttatgttttg tggttttaaa
    11341 tttattttat ggataaattg cgaagcggaa ttaattcgcg aagaaaagca gaaaaaacgc
    11401 cgcagagcgc ggcgtttggg ataatttgca tatttcttgc cggatgcggc gtaaacgctc
    11461 tatccgacaa cgtaaactta ctcgcctact ttcgcccagg tatcacgcag cccaacggtg
    11521 cggttaaata ccggtttttc cgccgtagaa tggcggctgt cgaggcagaa gtaaccttca
    11581 cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc agcaaagccc
    11641 tgtttgatca ccagcgactc cgggttaatc accgacagga aatcatccgc agcaccaggg
    11701 ttcggcacgc tgaacagacg atcgtacagg cggatttcaa ccggcagcgc atgtgccgcg
    11761 ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt gcttaaggtg
    11821 tcggcgtcat aagtacagaa gatggtggtg atattgcctt cggcatcttt ctccacgcgt
    11881 tctgccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac cagacgtttg
    11941 tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc accgctaaat
    12001 ggcacctgac ggctgcccat ttccggttta ttcggatggt tcggcatggt aaccatttcg
    12061 ccttcgccct ggtagttttc gataaccagt ttcaccggat cgataaccgc cattgcgcgc
    12121 ggcgcatttt cgttgagatc ttcacggatg caggattcca gtgacgccat ctcaatggtg
    12181 ttgtcctgct tggtcacacc aatgcgtttg cagaactcac gaatagaagc cgcagtgtaa
    12241 ccacgacgac gcagaccaga aatggtcggc atacgcgggt catcccagcc ttcaacgtgc
    12301 ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc cagattcagg
    12361 cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga tgttgtccag tacccagtcg
    12421 tacagacggc ggttgtcctg gaactcaagc gtacacagag agtgcgtaat accttccagc
    12481 gcatcgctga tgcagtgggt gaaatcgtac atcgggtaga tgcaccactt gttgccagtc
    12541 tggtggtgtt cagcaaactt aatgcggtac agcaccggat cgcgcatcac gataaacggc
    12601 gaagccatgt cgatcttcgc acgcaggcag gctttacctt cttcaaaacc gccggtacgc
    12661 attttctcga acagcgccag gttctcttca acgctgcgat cgcggtacgg gctgttttta
    12721 cccggctgcg tcaaggtgcc gcggtattcg cggatctgtt ccggcgtcag ttcatcaacg
    12781 tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc aaaataatcg
    12841 gaggagtaac ggacgttacc agaccagtgg aagcctaacc actctacgtc gtttttgatc
    12901 gaatcgacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg caggttgcac
    12961 tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc atggccaata
    13021 tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg cttaccactg
    13081 gccagatctt catcgatgat ctgacgaata aagttagtcg ggcgggcttc tgcctcactc
    13141 atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat aagcgggaca
    13201 ggctgacaac tgttagttag cgaaaaaatg gttttgcgta agatattggg gcgttttctg
    13261 cgctactgcc agcagatcct cgcgctggaa acgtaaggcc aaacgtcgcc agcctgacgc
    13321 atttattaaa ttgccggatg agctggccgc agagtcatag attagtcggc tatgattaaa
    13381 cgcgtcgaca aggacgcaag cgttctacgc gtctgcccat caagctgact atccgtaata
    13441 atctctgtca gttcattaag atgcgcgata ttaaacagcg accatgaacc gtattttgag
    13501 ctgtcagcca ggagaatttt gcgcgaagca tgcgcgataa gatcgcgttt tagtgcggct
    13561 ttctcttccg tgggtgctgt aatacccttt tcaagatccc agctgttaca gcttaaaaat
    13621 gccacgtccg gccaggtatt ttgcagcaat ttacgaccat gttcgccaat gcaggactgg
    13681 ctggagtcgt cgatacgtcc gccgataatc gtcacttcaa tttgcttgaa ttcagccaaa
    13741 aaaagcgcga tatgcaggtc gcttgtgatc acgcgaagcg gtaaatgtgt aatctgccgc
    13801 gcaagttcaa tcattgtcgt accggcatca aggacgacgg aatcccccgc tttgaccatt
    13861 gacgccgctg cgctggcgat ggcatgtttt tccgccaggc tgcgctgcat tttctcatgg
    13921 gtagtcggtt gggtaggaat aaaccggttg agggtcacac cgccatgtgt acggctgata
    13981 acaccctgct catcgagttt tatcaggtct cgacgaatcg tagccggcga cgcattggtt
    14041 accgccacca gctcatcaac ggtgaccaga ttatggcttt tcaggtaatc catgatctgc
    14101 tctaaccggt tatatccctt cattttattc tcgcgaaatc tgcattgcta actggatgga
    14161 aactgccatg ctctcagact tggcttttcc tgtccaggca atatcaaacg cggtgccgtg
    14221 gtcggcagaa gtgcgaataa atggcaaccc ggcggtgata ttcaccccat cataaaatcc
    14281 aagcagtttt aaagggatat gcccctgatc gtggtacata gccactacca tatcgaatag
    14341 cccttcatgg cattgcataa acacggtatc tggcgggcac gggccagtaa catcaatatc
    14401 ttcggcctgc attgcttcaa tcgcaggcgc gataatctcg atttcctcag tgccgaataa
    14461 accatgttcc cctgcatgtg gatttacacc cgccacggca atacgcggac gttcaatacc
    14521 aacacgtttc aggaagtggt tcgcaacacg aataaccgtt ttgacccttt caccattcaa
    14581 ggtatccagg aatttacgca gtgagatgtg agtcgaaata tggataacct tgagcttatc
    14641 ggtatagagc accatcgcgt actctttgct gccggtaaga tgggccagca attccgtatg
    14701 ccccggatag ttatgcccgc caagatgcag cgcttcttta ttcagcggcg cagtggctat
    14761 ggctttcacc tcgcctgata aggccagagc cgttgcacgt ttgatacagc gatacgctaa
    14821 atcgcctgcc gcagcctgta ccactccggg tgtcagcgca tcggggttag ccagcggctc
    14881 atccatcacg ttaatcacgc cgggagcaaa atgggcgtcg cacacctttt gaatgacgcg
    14941 taactctgcc tgcggcgtga tattcatcgc cataatacgt cgcagcgtgc gcacacagcc
    15001 caccaccacc accggggcac cagcaagccc ggcttcgctc agggatttaa taatgatctc
    15061 cgggccaata cccgccgggt cacccatcgt taccgcaata atattattca ctacacttct
    15121 cctcgataaa attcagaacc tgatgcaacg tggtctcatc gccaaaccca ccggctttcg
    15181 tcatcaccga acgggaccag acaccgccca gaaagtgacc gtatggaacg cattgcgcca
    15241 ccttccccgt aatacgaaat ccggtcgcgc ctaacgcact ggctgttgcc attgcgacat
    15301 caccaccaga gagataaagc gcatcgggca ttgtgcgaag cagaacttgc cgggtaagtt
    15361 cgccaagaaa ccgacaaatt ttttccccaa gtgcagcgcg actcatctgc caacgctggc
    15421 aaagggtgtc gatctgatgc cttgctacag aatcgttaca ggtgtggaca atgcagtggt
    15481 cgcctttctg caaggcctgc acaatacgcg cgtcatagtc actggcattg cctgccagaa
    15541 tgtgttcaac atcgacgtat atctgtgtga tacatgggtg tgaatgaagt gtggcgatct
    15601 gccgttgagc gatttcgctc attgaaccaa taatggccag cacggtgggt ggcggcataa
    15661 tcgcggagcg ccgggcaacg gcatcacaaa taccagcgga gccgaccagc agcagcggtt
    15721 gtttagcctg catcacagcg gcagcgatgc gatccaggtc gctgtccgtc tgcgcatcta
    15781 tcacgctaat ccacggttgc tcataagaca aatggctgag ctgagaaacc gtaacgggct
    15841 ggcatggaat ggccgtctgc aaccggatga tgtccgcaat tcgggcgctc aataccgggg
    15901 ttttcggatc gctggcaaac tcggtttccg ttatccgcac accgtttacc caacactcac
    15961 cggccactgt ggtccttccc gcctgaggaa atgcgggggc aaccactgcg cccgttatac
    16021 cgcatgcctt catcagggct tcaatttctg cgccgatatt gcctcgcagc gtggaatcta
    16081 tctttttaat cacgagcggc ggattattcg ccagagggag atgactcatc agcgaggtta
    16141 ttttcatcgc cgccagctta ggatccatcg cccgactgtc gctgttaatc acccatacgc
    16201 tggcatcacc gcgataaggc atttcaaagg ccacatcaac tgtatgcccc acctgcgcca
    16261 ggcttacccc cgcgtcgttg gctcccgtga aatcatcggc aacgacgaga atgctcggct
    16321 tagtgattcc cttttccatc atcactccag gttgttttat gataacaatg attatattca
    16381 atcatgattg attaaatgcg agcaatctca agttattaat cactcgaata aattataaaa
    16441 ttagaccatg ttacgtaggc tacggcattt tgaatgattg aattcaatca ccagaagaga
    16501 ggttatttgt ggtaattatc aatagcacga tactgagcgg cgcaggcgct atcccttccc
    16561 tgacgtcgct cttacccgac atcagaaaaa tgctgctggt cactgaccgt aatattgcgc
    16621 agctcgacgg tgtgcagcag attcgcgcct tactggaaaa gcactgcccg caggttaacg
    16681 ttatcgataa tgtgcccgca gagcccacgc atcatgatgt gcgccagcta atggatgtcc
    16741 ttggcgatgc ctcttttgat gtggtggtcg ggatcggcgg tggcagcgtg ttggatgtgg
    16801 caaagctgct atcggtgctt tgccatccac aatctccggg gctggatgcg ctgcttgcgg
    16861 gtgaaaaacc gactcagcgg gtgcaatcat ggttgattcc tacaaccgcc ggaaccggct
    16921 cagaagccac gccgaatgcg attctggcaa tccctgagca aagcacgaag gtgggtatta
    16981 tttcccaggt gctgttacca gactatgtgg cgcttttccc ggaactgacc accagcatgc
    17041 ccgcgcatat tgcggcgtcc acgggcattg atgctctttg ccacttactg gagtgtttta
    17101 ccgcgaccgt ggcaaatccg gtcagcgata acgcggcgct gactgggtta agtaaacttt
    17161 tccggcacat tcaacccgcc gtgaacgatc ctcaggatct gcgcgcaaaa ctggaaatgc
    17221 tgtgggcgtc ttactatggc ggcgtagcga taacccatgc gggcacgcat ctcgttcatg
    17281 cgctctccta cccgttaggt ggcaaatatc atctgccgca tggcgtcgcg aatgccatct
    17341 tgctggcgcc gtgcatggcg tttgttcgcc cctgggcggt cgagaaattt gcccgggtct
    17401 gggattgcat tcccgatgcg gaaaccgccc tgagcacgga agaaaaatct catgccctgg
    17461 tgacctggtt acaggcatta gtccatcaac tcaagctacc caacaatctc gcggatctcg
    17521 gcgtaccgcc agaggatatt gcctctctta gcgaggtggc actgaacgtg aagcgcctta
    17581 tgaacaatgt gccgtgccag cttgatctac aggacgtaca ggccatttac caaacactgt
    17641 ttccgcaaca tcaatttaag gaataaatca tgccgaaaac cattgaaggt gtattaaccg
    17701 ctatcgtcac cccgtttacc gcgaccggca gtctgaatat cccggcgtta aaagtgcagg
    17761 tgaatcgtca actggaagcg ggtaacgcga ttttttgcgg cggcaccaac ggcgagtttt
    17821 ttgtgttgaa tgaacaggag aagctcagcg ttacgcaaac ctgcgtggat gaagtggctg
    17881 gtcgtgcgcc ggtcgtggca cacattggcg agatctctac gcgcgaaacg attcgtctgg
    17941 gtcagcagat tgaaaaactt ggcgtagatg ccgtgtcggt cattgcccct tacttcgtac
    18001 cgctgaaaca ggaagagttg attgcgcatt acagcgcaat tgccgacgca ctgagcattc
    18061 cgatgtttct ctataacatc ccggcccgca ctggcaatac gatccagcca gaaaccgcgc
    18121 gtgctctggc gtcgcacccc aatatcattg ggattaaaga cagcgccgga agcgacgaaa
    18181 gcctgaaggg ttttctcgat gcggtgcgcg acattgacgg ctttaatgtg ctcaacggcc
    18241 cggattcgct gatccataaa gggtttgtcg agggatgttc aacctgtatc tctgggctcg
    18301 ccaacatcgc gccgcgagaa atcaatgcca tctggtctcg cttccatgcc ggtgatattg
    18361 aagggtctta tgaagcacag gagagtgtaa cgggcctgcg taccgatctt tataaagtag
    18421 cattctcccc ggctgcggtg aaaaaagcgt tgcagcttat ggggcatgat gttggtgata
    18481 gccgctacgc ggttaatttc acgcctgaac agatcagcga gattcaacaa attattcgtc
    18541 attataacat taattaaatt aactctatta tccagcccgc tataaaattt agataaagag
    18601 aataaaggat taccccatgt ttttgtggtt tatgcagcca gatatctggc tcactttaat
    18661 taccctcacc tttctggaaa ttattcttgg ggtggataat attatttttc tttcactggt
    18721 ggtggcaaaa cttccgttat cacaacaaaa tatggcgcgt aaactgggtt taagctgcgc
    18781 gatgattatg cggatattat tgctgatatc cattgcgtgg ctttcacata ttacccagcc
    18841 gctttttacc gtgctgacgc tggagatttc gttccgcacc ttgattctgc tcgggggcgg
    18901 gctgttcctg atttataaag cgatacagga gatacgcgaa gagctgtcgc ccgcgcagga
    18961 agacgaacat ggcagcggat cgcggcaact gtcgttgtgc ggtgccattg tacagataat
    19021 gctgctcgat atcgtgttta gcctggattc ggtgatcacc gccgtcggcc tttctcagca
    19081 tatatttatc atgattgccg ccgtaatgat tgccgtcggt gtcatgatgt ttgcggcgaa
    19141 aacaattggt gatttcgtta acgccacgcc gtcaataaaa atactggcgt taaccttttt
    19201 attgtttgtc ggtgtattgc tggttgccga tagtctcaac attcatattg ccaaagagta
    19261 tctctacttc gcgatatttt tctcattaag tgttgagaca ctcaatatta tccgtgaaag
    19321 aaaaaataaa aaagtttcga cattgatcga aacgcatgaa tcgcactcgc attaataaat
    19381 ctattaccaa ctttaagatg gagtctaact atgtctgtag cccttattcg tgacggcgtc
    19441 atccgtccat ccgaaaacga ttcgcatgtg cttaccgcca tgctgccctc gtcctgcccg
    19501 caaaaccacg cggcgaatat tctgcctttg ccggatggcg cgttaatgtg cgtctggttt
    19561 gcgggaacgc aggaaggtat tgccgatatt tctgtctggg gatcgcgatt gcccgctggc
    19621 ggaatgcagt ggagcgatgc ggtcaaactt tcccacgatg acacgcgttc agagcaaaac
    19681 ccggtgctgt ttctcgcgcc ggataacgtg ctgtggctcc tatggaccgc acaaatatcc
    19741 ggtaatcagg acaccgccat cgtccgctac cgtaagtcag acgacctcgg acaaacctgg
    19801 ggcgagattg ccaccctgct tgataaaccc ggtacgttca ttcgacagcc aatcacggtg
    19861 cttgataacg gcaactggct gctgcccgtc ttttattgcc gtacccaacc cggcgaaaaa
    19921 tgggtcggca atgatgatat cagcgcagtg aaaatttccg ctgacggtgg tcactcctgg
    19981 cgcgatgtcg aggtgccgca aagtcttggc tgtgttcata tgaatatcac aatgctgcac
    20041 aacggtacgt tggttgcttt ctttcgcagc cgttgggcag acaacatcta tatcagccat
    20101 tcagcggaca acggcgagag ttggtcggtg ccgcaagcaa ccgaattgcc caacaacaat
    20161 tcgtctattc aggtaacgac gcttgccagc ggcgaactgg cgctggtgta caacgcgatg
    20221 agtgcggcag gcgcagtaga acatcgcgcg tcgctttatg acgaaatcga cgatggcgac
    20281 gacagccgca aagaaccgac agccgtcgga cgctccgctt tctggggtgc cccacgcgcg
    20341 ccgatgacgg tagcgatttc tgccgatgga gggaaaagct ggccgtggcg gcgcaatctc
    20401 gatgaaggcg acggctactg catgaccaac aattccctgg aaaagctaaa tcgtgagttc
    20461 tcctacccca gcatcaaaca aagcccggac ggtacgctac atatcgctta cacttggtgg
    20521 cgtcaggcca tcaagtacgt acgcatctcc cccgagtggg ttaaggggca ggcatcatga
    20581 ttatcggtaa tctcaaccat ttgtcgctgg cggggcttcc tgcctgggtg agaaatattc
    20641 tgctgcgccc ggagtgttcg ttatcggcgt tatccaccag agaggacggt cgctggcaac
    20701 ctgaaggatg tcggtggttc tgcacgctgg gaacttcaga cacgcaaccc gccgaactcc
    20761 gccatacgga gtatcaccat ctatgggccg atatacaggt ggtgattact ggttgtgagg
    20821 gcattaatgc gggaacgcgg cccatcgctc gcgaaaacga tgaagaacgt aagcccgatc
    20881 tgtttatcgc cccatcccct gaaaacagtg tcgccatcac cctacacgcc ggtgatttcg
    20941 ccgtctttat gcctgaggaa cctcaccagg cgctgtgcgc aattggcacg gctacttcaa
    21001 tacgcaaagc ggtctttaaa gtaccgcgcg acatgctgga agcctaaaag gagagaaaaa
    21061 atgacaatgg aaatgatcct ggcattagga atactggtcc tgatgatcgt gctgatcatg
    21121 tcggacaaaa tgccttttgg cgcaccgcca ctgcttgcgt gtttattgct ggttgtatcg
    21181 ggactttcga ccgttcaaca ggcttttgcc ggattcgtca atccaagcgt ggtgatgatt
    21241 gcagggttta tggtggtcat ggcggcgcta caaaaaaccc ggcttatcag caacgttaaa
    21301 tctgcgatga taagcctggt caataaaggc agttaccgca gctatgggct gttgttagtc
    21361 attgttatgc tgggtgccag tcttgccggc accggagcga ccggttatta cgtgctaatt
    21421 ctgtcgctgg tatccaccat tccgtacagt aaaaagctac caacatcgaa attaatgatg
    21481 cccctgggat ttgccaccaa ccacccgctt cttcctatca atctcgcgct tctctttggc
    21541 gttacggcga ccgtactcga aaccgcgggg tttcatcagg agatttcgat ggggcgcttt
    21601 gcgctggtga atctcatcat gtcagcggct ttcctcgcat ggagtcttat tgcctaccgt
    21661 tttcttccgg atcatcccat tgcggatgct tccgaggatg cgctggcggc acgcgaggaa
    21721 acgtttaacg cccttcctgc ctggaaagaa tattgcacta ttgccgcatt cgctgtcagc
    21781 gttatcggca tgatgctaat gaacattctg ggaaatatcg ccttcgttat tccgggtctt
    21841 gcgggtgcgt ttgttctgat gatcgatgta ctggacttca aagaagttcg cgatcatatg
    21901 ggggctccgg tcattttaat gatggcgggg gtgatcggtg tagctgatgc actggcgggc
    21961 accggcttta cggcgatggt tggcgatgcc gttgcaggtg tgctcggttc tggcgtctca
    22021 ccgtttgtat ttattgtcgc ctttgctttg cttaccagta cctgcgccac cttcacgggt
    22081 tcgaatatgg ggtcagtata tatttttgcc cccatcgcca ttgccgcctg tactagcctt
    22141 gggcttaacc cgaccgctgc cgcgattgcg gtggtgattt ctggctggaa tggcggctat
    22201 atgcctattg acggaatgcc agccatgatc ctcggaatgg ggaaatacaa acttcccgaa
    22261 ttctgggtgt tctctgtacc aatgtatctc atccgcattc tggcgctttg tgcaggcgct
    22321 gtttttatct tcccaatgta atctcagaca aaaaaagcgg cagagaagat ccctgccgct
    22381 ttttattgtt ggatgcgacg ctgccgcgtc gcatcagaca taaagcagat tactttttga
    22441 tttcatacag cggtgtttga cccgccacaa catggccctg agctttaatg ataaggccgc
    22501 tgaaatcgtc gatattgctg caaaccaccg ggctaatcat cgagcgagcg ttagcgttca
    22561 ggtaatccag atccatttcc agaatcggtt gccctgcgct tacctgcgcg ccctcttcca
    22621 ccagacgttt aaagccttta ccttccagcg ctacggtgtc gatacccata tggacgacga
    22681 tctccgcgcc tttttcggtt tccagacaga atgcgtggtt ggtgttgaag attttcacga
    22741 ttgttcctgc ggctggtgat acgacgattt tatctgtcgg tttcaccgcc acaccgtcac
    22801 ccaccgcttt gctggcgaat gcttcgtcag gaacctgatc cagtgccaca acatccccgg
    22861 taatcgggga taccagctcc gcgatagata ccgcgtttgg tacagcctgc ggttttgcta
    22921 caggagcggc agttgccgga gttgcttcag ctgacgcagc ggctaccgga ccacgggcaa
    22981 cgactttctt catcgcatcg ccgatggatt ctgctttcgc gccaacaatc acctgaatag
    23041 tctgtttgtt cagtttcact accccagaag cacccagacg tttacacatc gcatcgttga
    23101 cgcgagctga gtcaaccacg gtaaggcgca gacgagtaat acaggcatca atggctttca
    23161 ggttgtcagt accgccaacc gcagcaatat agttggttgc cagttgattg agaccttctt
    23221 cagtattgct gttagcctct tcagtaacga tctcgtcttc tttatcttca cgacccggcg
    23281 ttttcaggtt gaacatgcgg ataaccaaac tgaacaccac gaagtagata gcgaagaaga
    23341 caacccccat caccagcagc atccagacgt tctggctggc ggccggcaga ttatacatca
    23401 acgcgtagtc gatagccccc gcagagaaag agaagccggc atggataccc agcaacgttg
    23461 ccacaaacag gctgataccg gtcagcagtg cgtgcaggag gtacagcagc ggtgcaagga
    23521 acatgaacag gaattccagc ggctcagtca caccggtcag gaacgcagta acagcaacag
    23581 aaagcagcat cccgccaacc atcggacgac gctctttcgg tgctgcgaag tacatcgcca
    23641 gcgccgcacc cggcagaccg aacatcataa tcgggaagaa gccggacatg aacatccccg
    23701 cggtgccgtc accggcatag aagcggttga tatcaccgtg gaaaaccgtt cccgccgcgt
    23761 tggtgaattc accaatctgg aaccaggcga tggtgttcag cacctgatgc agaccggttg
    23821 ggatcagcag acggttgatg aaaccaaaga taccggaacc cagcgcgccc gcagaaacga
    23881 tccactcgcc gcctgcatgg atagcgtgct gtaccggcgg ccagacgtaa ccaaaaatgg
    23941 ccgccagcac cagacagaag aagccggtgg cgatcggcac aaagcgtttg ccgccgaaga
    24001 agctcaggaa gtccggcagt ttaatatcgg accaacggtt ataggctgcg ccaccaacca
    24061 gaccggtaat gatacccgcc agtacaccca tgttaatttc tgggttgatg gtcaccatcg
    24121 ctttggttaa cacaaagtaa cctaccgcac ccgccagtgc cgccgcacct gcgctgtctt
    24181 tcgaccagct ggatgccacg ccgatggcga agattaatgc gaggttatca aaaatcgcac
    24241 cgcccgcctg ggcaataaac gcaacgttaa gtaaatctgg ctgaccgaat cgcagcaaca
    24301 gcgccgccac cggcagcacc gcgataggga gctgtaacgc cctaccgagt cgctggaaaa
    24361 aacctaaaat attcatctta ttccccctac gagaacccta tttggctcgt ttaaagccgt
    24421 atttttattt tgctgcaaat tgtactgccg atgttctgta atcagattgt tagatcatct
    24481 gctacagagt gtgtgaaaat ttaattcgta tcgcaaatta aacgcgtgtc ttttgtgagt
    24541 tttgtcacca aatatcgtta tcatcactcc cttttactgg ctaaaccaga aaacttattt
    24601 tatcattcaa aaaatcaggt cggattgacg cctgtctgcg caaatccagg ttacgcttaa
    24661 agatgcctaa tccgccaacg gcttacattt tacttattga ggtgaataat gagactgatc
    24721 cccctgacta ccgctgaaca ggtcggcaaa tgggctgctc gccatatcgt caatcgtatc
    24781 aatgcgttca aaccgactgc cgatcgtccg tttgtactgg gcctgccgac tggcggcacg
    24841 ccgatgacca cctataaagc gttagtcgaa atgcataaag caggccaggt cagctttaag
    24901 cacgttgtca ccttcaacat ggacgaatat gtcggtctgc cgaaagagca tccggaaagc
    24961 tactacagct ttatgcaccg taatttcttc gatcacgttg atattccagc agaaaatatc
    25021 aaccttctca acggcaacgc cccggatatc gacgccgaat gccgccagta tgaagaaaaa
    25081 atccgttctt acggaaaaat tcatctgttt atgggcggtg taggtaacga cggtcatatt
    25141 gcatttaacg aaccggcgtc ttctctggct tctcgtactc gtatcaaaac cctgactcat
    25201 gacactcgcg tcgcgaactc tcgtttcttt gataacgatg ttaatcaggt gccaaaatat
    25261 gccctgactg tcggtgttgg tacattgctg gatgccgaag aagtgatgat tctggtgctg
    25321 ggtagtcaga aagcactggc actgcaggcc gccgttgaag gttgcgtgaa ccacatgtgg
    25381 accatcagtt gtctgcaact gcatccaaaa gcgatcatgg tgtgcgatga accttccacc
    25441 atggagctga aagttaagac tttaagatat ttcaatgaat tagaagcaga aaatatcaag
    25501 ggtctgtaat tgttatccct gccctggctc cttgctcagg gcattatttt ttaaaatcgg
    25561 gggtcagaat gtatgcatta acccagggcc ggatctttac cggccacgaa tttcttgatg
    25621 accacgcggt tgttatcgct gatggcctga ttaaaagcgt ctgtccggta gcggaactgc
    25681 cgccagagat cgaacaacgt tcactgaacg gggccattct ctcccccggt tttatcgatg
    25741 tgcagttaaa cggctgcggc ggcgtacagt ttaacgacac cgctgaagcg gtcagcgtgg
    25801 aaacgctgga aatcatgcag aaagccaatg agaaatcagg ctgtactaac tatctgccga
    25861 cgcttatcac caccagcgat gagctgatga aacagggcgt gcgcgttatg cgcgagtacc
    25921 tggcaaaaca tccgaaccag gcgttaggtc tgcatctgga aggtccgtgg ctgaatctgg
    25981 taaaaaaagg cacccataat ccgaattttg tgcgtaagcc tgatgccgcg ctggtcgatt
    26041 tcctgtgtga gaacgccgac gtgattacca aagtgactct ggcaccggaa atggttccag
    26101 cggaagtcat cagcaaactg gcaaatgccg ggattgtggt ttctgctggt cactccaacg
    26161 cgacgttgaa agaagcgaaa gccggtttcc gcgcggggat tacctttgcc acccatctgt
    26221 acaacgcgat gccgtatatt accggtcgtg aaccgggcct ggcgggtgcg atcctcgacg
    26281 aagctgacat ttattgcggt attatcgctg atggcctgca tgttgattac gccaacattc
    26341 gtaacgctaa acgtctgaaa ggcgacaaat tgtgtctggt taccgatgcc accgcgccag
    26401 caggtgccaa cattgaacag ttcatttttg cgggtaaaac aatatactac cgtaatggac
    26461 tttgtgtgga tgagaacggt acgttaagcg gttcatcctt aaccatgatt gaaggcgtgc
    26521 gtaatctggt cgaacattgt ggtatcgcac tggatgaagt gctgcgtatg gcgacgctct
    26581 atccggcacg tgcgattagc gttgagaaac gtctcggcac gctcgccgca ggtaaagtag
    26641 ccaacctgac tgcattcaca cctgatttta aaatcaccag gaccatcgtt aacggtaacg
    26701 aggtcgtaac tcaataagag aaagtatgac accaggcgga caagctcaga taggtaatgt
    26761 tgatctcgta aaacagctta acagcgcggc ggtttatcgc ctgattgacc agtacgggcc
    26821 aatctcgcgg attcagattg ccgagcaaag tcagcttgcc cccgccagcg taaccaaaat
    26881 tacgcgtcag cttatcgaac gcgggctgat caaagaagtt gatcagcagg cctccaccgg
    26941 gggccgccgc gctatctcca tcgtcaccga aacccgcaat ttccacgcaa tcggcgtacg
    27001 gcttggtcgt cacgacgcca ccatcacctt gtttgatctc agcagcaaag tgctggcaga
    27061 agaacattac ccgctgccgg aacgtaccca gcagacgctg gaacatgccc tgctgaatgc
    27121 cattgctcag tttattgata gctaccagcg caagctacgc gagctgatcg cgatttcggt
    27181 gatcctgcca gggcttgtag acccggacag cggcaaaatt cattacatgc cgcatattca
    27241 ggtagaaaac tgggggctgg ttgaagcact ggaagagcgt tttaaagtga cctgttttgt
    27301 cggccacgat atccgcagtc tggcgctggc agagcactac ttcggtgcaa gtcaggattg
    27361 cgaagactcc attctggtgc gtgtccatcg cggaaccggg gccgggatta tctctaacgg
    27421 gcgaattttt atcgggcgca acggcaacgt cggtgaaatt ggccatattc aggtcgaacc
    27481 gctgggtgaa cgctgccact gcggcaactt tggctgtctg gaaactatcg ccgccaatgc
    27541 tgccattgaa caacgggtgt tgaatctgtt aaagcagggc taccagagcc gtgtgccact
    27601 ggacgactgc accatcaaaa ctatctgcaa agccgcgaac aaaggcgata gcctggcctc
    27661 ggaagtgatt gaatatgtcg gtcgtcatct gggtaaaacc atcgccattg ctatcaactt
    27721 atttaatccg caaaaaattg ttattgctgg agaaatcacc gaagccgata aagtgctgct
    27781 ccctgctatt gaaagctgca ttaataccca ggcgctgaag gcgtttcgta ctaatctacc
    27841 ggtggtacgt tctgagttgg atcaccgctc ggcaatcggc gcttttgcgc tggtaaaacg
    27901 cgccatgctc aacggtattt tgctccagca tttgctggaa aattaatgtg cttttatagt
    27961 ggcgcttatt gttgtcaata ttctgggtag tccatgacca ttaaaaatgt aatttgcgat
    28021 atcgacggcg tgctgatgca cgataacgtc gccgtacccg gtgcagcgga atttttgcac
    28081 gggattatgg ataaaggcct gccgctggtg ttgctgacca actatccttc acagaccggg
    28141 caagatctgg cgaatcgctt tgccactgcc ggtgtcgatg taccggatag cgtgttttat
    28201 acctccgcga tggcgaccgc cgatttcctg cgtcgccagg aaggcaagaa agcgtatgtg
    28261 gtgggcgaag gcgcactgat tcatgaactg tacaaagccg gtttcactat taccgatgtg
    28321 aaccctgatt ttgtgattgt tggcgaaacg cgttcctaca actgggacat gatgcataaa
    28381 gcagcctatt tcgtcgctaa cggtgcacgc tttatcgcca ccaacccgga cacctacggg
    28441 cgcggttttt atcccgcctg tggcgcgttg tgtgcaggga tcgagaaaat ctccgggcgc
    28501 aaaccgttct atgttggaaa gccgagtccg tggatcatcc gcgcggcatt aaacaaaatg
    28561 caggcgcatt cggaagaaac ggtgattgtc ggcgataact tgcgtaccga tattctggcg
    28621 ggcttccagg ccggtctgga gacgattctg gtgctttctg gtgtttcgtc gctcgacgat
    28681 atcgacagta tgccgttccg cccaagttgg atttacccat cggtcgctga aatcgacgtt
    28741 atctgaatag caaccacgcc tcagggcgtg gtttcttatt tttacccatc attaaaatca
    28801 ccctattcaa taaaacccgt caaaaattat cgatccatca ataaatatac ccaaagcccc
    28861 tccccttttt catcaatcag caatcgccat tacgtttttt atttttccac tgccaaatcg
    28921 cttgcgcacc gtgctgcttt gcggcatttt tttaacaagt aaacacaaca cgcagtaaac
    28981 accaggttaa cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac
    29041 gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg
    29101 gactggtccg gtatttatgc cagcgataac gccatcctcg cccacgaacg tctgtcaatt
    29161 gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaactca tgtgctggca
    29221 gtaaacggtg aaatctacaa ccaccaggca ttgcgcgctg aatatggcga tcgttaccag
    29281 ttccagaccg ggtctgactg tgaagtgatc ctcacgctgt atcaggaaaa agggccggaa
    29341 tttctcgacg acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgca
    29401 tatctgattg gccgcgacca tctggggatc atcccactgt atatgggcta tgacgaacac
    29461 ggtcagctgt atgtggcctc agaaatgaaa gccctcgtgc cagtttgccg cacgattaaa
    29521 gagtttccgg cggggagcta tttgtggagt caggacggcg aaatccgttc ttattatcat
    29581 cgcgactggt tcgactacga tgcggtgaaa gataacgtga ccgacaaaaa cgagctgcgt
    29641 caggcactgg aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgctg
    29701 ctttctggtg gtctggattc ctcaattatt tccgctatca ccaagaagta cgccgcccgt
    29761 cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc agttgcactc ctttgctgta
    29821 ggtctgccgg gttcaccgga tctgaaagcc gcccaggaag tggcaaacca tcttggcacg
    29881 gtgcatcacg aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt
    29941 taccacatcg aaacctatga cgtgacgaca atccgcgctt caacaccgat gtatttaatg
    30001 tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg ttctgacgaa
    30061 gtgtttggcg gttatctata cttccacaaa gcgccgaacg ctaaagagtt gcatgaagag
    30121 acggtgcgta aactgctggc cctgcatatg tatgactgcg cgcgtgccaa caaagcgatg
    30181 tcagcctggg gcgtggaagc acgcgttccg ttcctcgaca aaaaattcct tgatgtggcg
    30241 atgcgcatta acccacagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg
    30301 cgtgaatgtt ttgaagcgta tctgcctgca agcgtggcct ggcggcaaaa agagcagttc
    30361 tccgatggcg tcggttacag ttggatcgac accctgaaag aagtggcagc gcagcaggtt
    30421 tctgatcaac agctggaaac tgcccgcttc cgcttcccgt acaacacgcc gacctcaaaa
    30481 gaagcgtatc tgtaccggga gatctttgaa gaattgttcc cgcttccgag cgccgctgag
    30541 tgcgtgccgg gcggtccttc cgtcgcttgt tcttccgcta aagcgattga gtgggatgaa
    30601 gcgttcaaga aaatggacga tccgtctggt cgcgcggttg gcgttcacca gtcggcatat
    30661 aaataagacg tgtaatattg ccccggagaa atccggggca atttcagtta ctggggcgct
    30721 tgttgattct gatatgcagt aaggaaatgt tccgggtttg ccacatcgat gtttgacaac
    30781 tcattactgt aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa
    30841 tttcatcctg tatgacaaaa gaatggctgt agggtgggga aaaaacacaa agtaaacaat
    30901 aattgacgaa tatagcgcca tgctgttcgc aacctaacca aacagtcact ttcgagcaat
    30961 tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc
    31021 cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    31081 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tttgcgggag
    31141 tggcgaaatt ggtagacgca ccagatttag gttctggcgc cgcaaggtgt gcgagttcaa
    31201 gtctcgcctc ccgcaccatt caccagaaag cgttgtacgg atggggtatc gccaagcggt
    31261 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatatt
    31321 cttcgaataa gcggttcacc gcccggttat tggggtatcg ccaagcggta aggcaccggt
    31381 ttttgatacc ggcattccct ggttcgaatc caggtacccc agccatcgaa gaaacaatct
    31441 ggctacgtag ctcagttggt tagagcacat cactcataat gatggggtca caggttcgaa
    31501 tcccgtcgta gccaccaaat tctgaatgtg tcgaatatgt tcggcaaatt caaaaaccaa
    31561 tttgttgggg tatcgccaag cggtaaggca ccggattctg attccggcat tccgaggttc
    31621 gaatcctcgt accccagcca atttattcaa gacgcttacc ttgtaagtgc acccagttgg
    31681 ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt tcgaatcctc
    31741 gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc tgcgtatatt
    31801 caatgccgaa tgtgatgttg actcgtctta tccttcaaag tcggatgcga cgctgccgcg
    31861 tcttatccga cctacggttg gcatgcatcc agcaatgttg tgaggctaca accctaacgc
    31921 atatttcagc gccttacgtt tcaacacgcc agcacgctcc gccgccatca aaccaagatt
    31981 acgcacaaaa cgcagcggtg gcagattatt gctgaatccg gcataaaaca gatccatacc
    32041 actttgcatc atgaagttat ccgccatacg ccgcatctgg taacgcttga gcacaggata
    32101 actggcccac gcttcgccgt agctgcgggc gttcaccaga acgtcaatca gggcatcgac
    32161 atcacgataa ccaagattca ctccctgtcc cgccagcgga tggatggtat gcgcggcatc
    32221 gcccaccaga gcaagccctg gttgcacata ctgcaacgca tggcgacgta tcagcggaaa
    32281 cgcaccagcg gcaagcggtg tcacgtaacc tagacgcgac gggaaatgct tcgcgatttc
    32341 cgcctgtagc tgcgccatat tcatattctg caactggcga atgcgcgccg gagagtcata
    32401 ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt
    32461 aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa tcaacataca
    32521 cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgtcgca cctgcgaatt
    32581 tgcgccgtcg gcaccaatca ccagcttcgc gaggatcgtt tcaccgccct tcagttccag
    32641 ctcctgaaga tcattatggc gatgcagtgc aatcagcgag gttggcacac gtaacgttac
    32701 tttcggatgc gcttccagcg cctgccacaa cgcctgttgc aggacagtgt tttccaccat
    32761 gtagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg ccgtttccca
    32821 ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg catcccagac
    32881 ccctaaccct ttaagcaatg ataccgaagc cgcactaatc gccgagatcc gcacgtccgg
    32941 ctggctgtca gcgacaaacg gtgcaggttg ggcatgttcg ataaccatta ccgaaaatcc
    33001 gtgctgagcc agtcccagcg ccagtgcgcc gccgaccatt cctccgccga caatggcaat
    33061 ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt aagtttacag
    33121 gatttttacc cccaagggct gataacgctc atactggtca caacggcagc aaagcattac
    33181 actatgcgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga ccaaaaaact
    33241 ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga tggccgatct
    33301 gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg atgtgctgct
    33361 gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg
    33421 ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggcg gctgcgtggc
    33481 atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta tttttgggcc
    33541 gcaaacgctg catcgtctac cggagatgat caactccgtg cgcggcaacc gcagcccggt
    33601 tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctgccagaac cgcgcgccga
    33661 agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca cctactgcgt
    33721 ggtgccttac acccgtggtg aagaggtaag ccgtccgtcc gacgatattc tgtttgagat
    33781 cgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc
    33841 ctggcgtggt gagaactacg acggcaccac cggaacgttt gccgatctgc tgcgtctggt
    33901 tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga tcgaattcac
    33961 cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc tgcacctgcc
    34021 ggtacagagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca cggcgctgga
    34081 gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga tcagttctga
    34141 cttcatcgtt ggcttccctg gcgaaaccac cgacgacttc gagaaaacga tgaagctgat
    34201 tgccgacgtc aatttcgaca tgagctacag ctttatcttc tccgcacgtc cgggtacgcc
    34261 agccgccgat atggttgatg atgttccgga agaagagaag aagcagcgtc tgtatattct
    34321 gcaagagcgc attaatcagc aggcgatggc atggagccgc cggatgctcg acactaccca
    34381 acgtattctg gtggaaggga cttcacgtaa gagcatcatg gagctttccg gtcgcactga
    34441 aaataaccgc gtggttaact tcgagggcac accggatatg atcggtaaat tcgtcgatgt
    34501 agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgaa ctgaagatga
    34561 aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc gcaaagaaaa
    34621 cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc ggtgggcctt
    34681 gttttttctc tctttatccc catctttatc gcaatgtttg cgcctttttc ctgtggcgtg
    34741 aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga taagaggaac
    34801 ggtttgaaca tagacactcg cgaaatcacc ttggagccag cagacaacgc acgtctgttg
    34861 agcctgtgcg gcccgtttga tgacaacatc aaacaactcg aacgccgtct cggcatcgaa
    34921 atcaatcgcc gtgataacca ctttaaactg accggccgtc cgatttgcgt caccgctgcg
    34981 gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca gattcaggat
    35041 atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gggtactgga gcaaagcgcg
    35101 gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag
    35161 ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga cattaccttc
    35221 ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc agttgatgcc
    35281 ctggagcgtc aggaaattcg ccgtattctg ctgactcgtc cggcggtcga agccggtgag
    35341 aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct gcgcccgctg
    35401 tacgacgcgc tgtttgaaat gctgggcttt gagaaagtcg agaaactgat tgagcgcaac
    35461 gttattgaag tcgcaccgct ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc
    35521 attcttgatg agagccagaa caccaccatc gaacagatga agatgttcct gacccgtatc
    35581 ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct gccgcgtaat
    35641 accaagtctg gtttgcgtca tgccatcgaa gtgctggctg atgtcgaaga gatcagcttt
    35701 aacttcttcc acagcgaaga cgtggttcgt cacccggtgg tggcgcgtat tgttaacgcc
    35761 tatgaagcct gggaagaagc cgaacaaaaa cgtaaagcgg cgctggcagc agaacgcaag
    35821 cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg gcatgtgaag
    35881 ataattccgg gttaccggaa gagagccagt ttcagacatg gctgaatgcg gtgatcccgc
    35941 agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa agccacagtc
    36001 tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc ccgtttgaag
    36061 taccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt caggtggttg
    36121 agaaggaagc tcaggagcaa agcaaaccac tggaggcgca ctgggcgcat atggtggtgc
    36181 acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca gaagaaatgg
    36241 aagccatcga aacagagatt atgcttgctc tgggctatga ggatccgtac attgccgaga
    36301 aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga ttaacgacgg
    36361 cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca attcacacag
    36421 tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc aacttttcca
    36481 cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg ggcagaacga
    36541 ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg cagaccaacg
    36601 cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca accagacgct
    36661 ggacgaatgc cttgatgtca tcattgagtc cgcccactca cgtttcccgg tgattagcga
    36721 agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag
    36781 cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag
    36841 taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt
    36901 cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc tggaactgat
    36961 tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc gtcagctgag
    37021 tcgtcatacc tggactgtgc gcgcactggc ttctattgaa gacttcaacg aagcgttcgg
    37081 cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc aggcatttgg
    37141 gcatcttccg gcgcgtggcg aaaccatcga catcgacggt taccagttca aagtggcgat
    37201 ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact caccccagcc
    37261 gaagctggat gaataaaacc gaaactggat agataactac atggcttttg cctcattaat
    37321 tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg gaacgctggc
    37381 cttctctcct tacgacgtct ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt
    37441 gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg gatttggcct
    37501 ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg gaatgcctgg
    37561 cccggttaac atcttcctgg tggtactgct ggcggcgtat ttgtcgctgt ataccggact
    37621 gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc
    37681 cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtgctga ctggcttccc
    37741 gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg caccgataat
    37801 gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg cactggcgtt
    37861 ggtcaaacgc aactggcgtc cgctggtggt gaccgtcgtg ctgtttgccc ttcccttccc
    37921 gctgcgttac atccagtggt ttaccccgca accggagaaa accattcagg tttcgatggt
    37981 tcagggcgat attccgcaat cgctgaaatg ggacgaaggc cagcttctta atacgctgaa
    38041 gatttactac aacgcaacgg caccgctgat gggcaaatca tcgctgatta tctggccgga
    38101 gtcggcgata accgatctgg aaattaatca gcaaccgttc cttaaagctc tggacggtga
    38161 gttgcgcgat aaaggtagct cgctggtcac cgggattgtc gacgcgcgtc tcaataaaca
    38221 gaaccgctac gatacctaca acaccatcat cacactgggt aaaggcgcac cgtacagcta
    38281 tgaatcagcc gatcgctata acaaaaacca tctggtgccg tttggcgagt ttgtcccact
    38341 ggagtcgata ctgcgtccgt tagcaccgtt ctttgatttg ccgatgtcgt cattcagccg
    38401 cggaccatat attcagccac cgctgtcgtt gaatagcatt caacttactg cggccatttg
    38461 ctacgaaatc attctcggcg agcaagtgcg cgataacttc cgcccggata ccgactatct
    38521 gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc agcacttcca
    38581 gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca ccaacaacgg
    38641 cattacggcg gtgattggcc cgcagggtga gattcaggcg atgatcccgc agttcacccg
    38701 cgaggtgttg accactaacg tgacaccaac caccggactc acaccgtacg cacgaaccgg
    38761 caactggccg ctgtgggtgc tgacggcgct gtttggtttt gccgctgtac tgatgagtct
    38821 gcgtcagcga cgtaaacgtt gatgtgaatg atgcgctggc ctgcaaaatt gcatcaattt
    38881 tgcaggccag ataaaccgtt cacaccgcat ccggtatcaa caaaacatac tttctcattg
    38941 agttgtgggg catgattatc atgccctttt ttcttacggt tcagggatac aagccacatc
    39001 gccatattgg ggatgccgaa taattgcctg cggatcaagc gagcgcgtcc tgtccatttg
    39061 gattaacctg ttcttcgcca taacaaatat ctttctacca tccgctgaaa aacgaagatt
    39121 attaacgtag ctggcgggat aaacgccaat aatctgcccg gcattaatat tgagcaggat
    39181 gacataacct tgcaggccat atgacaccaa cgctaaatac tgaccgtccg ggctaatacc
    39241 ctcaacggaa taaacgctcc tgctacacca ttgcgtcata atgctggctt gttgatcctc
    39301 agctcttata cgtgaaatcc cctggctatc ggcaaaaata acctcaccat tttttggtcg
    39361 gaatcccatc gcagaaacga caccacacgc atcacctgcg ggaaagtctt ctttctgatg
    39421 cgtgatggta ttgatccgca cgatgttacc tctgcctgct cccggaacca gtaatgtttt
    39481 ttcatccggg gaaagtgcca taacccaggc gccaggttta ccgctcatcc ccgtcccgtc
    39541 gtatttctcc agcgggatat ccgccacatg ctgataagcc accagatcaa tcgctgacac
    39601 actattgctg gtgcgattca tcacccaggc ttttgtgcca tcacgcgaga ggataaagct
    39661 gtcaggatgt gcgccggtgg taatcgactg aataatctcc cccgtcgcca cgttaatgac
    39721 cgataacgca ttcgcttcaa atgaagtcgc ataaatcaac ttttcatcca ggcttaatgc
    39781 cagcgatctg acgccctgat ctttacagcc accacaatgt ttgactgact tttctttcgc
    39841 tatgggcgct gtaaaggtat gcaggatttt cttttgtccg aggtcgaaaa caacaatgct
    39901 gttcgtgttt tcaattgcgg catacaacgt attaccatcc cgactgatcg ccatatcatg
    39961 gatatcggca atctcagcga gtaaatcacc actggcctgc tcacgactta ccggagcgga
    40021 taatgtttcc ctgagggcgt tgcgctcctc cactaactgc tgcttttttt gtcgtcgctc
    40081 ttgttcaatc tcctgcggat aaaacgcttc acgcaggctt tgcctgacct gtaaaactga
    40141 ttcatcaatc tgctgctgca caaaccagat agtcccggtc agaagaagag tgaccgcaac
    40201 aataatcccg ccttccgtaa cgagccgatt ttgcccaact aaacgcgcac tcagccaacc
    40261 caacgcgccc cccgcaatca ggccgccaat gtggcaagcg ttatctattc ctgtttgccg
    40321 ggtaccatac aacagtgtca gcgccaccat acccaacaga ggaaatactc gccgctggtt
    40381 tttatggtgt gtgcccgcac ctgaagcaag ctgtgttgct atcgcagcgc ccgctattcc
    40441 cataattgcc ccggatgccc caacgctgat gactatctgc tcactttcgc gtaacgcata
    40501 acaggcgctg atcagcccac caaagatgcc ggagaataac cagataatca ggagtcgcta
    40561 tttccccagt atccgttccg ctactgtccc caccgccagg agtgcaaaca tattcatcag
    40621 caaatgggaa aaactactgt gcagaaaaac actgctcaat aagcgccagg attcatccga
    40681 caacgtgaga gaagcaacgt tagcgccgag atggatgagt gcggattgat cgacttcttc
    40741 gtaactcatc cattgtgcga ggtaacaaaa tatcccaaca cagagcgcaa aaaggatcag
    40801 ggttattgcg ggtaattgaa catttaacgg cttaaccgaa gatgcagaca taaggtgatt
    40861 cttccttgaa aagactagta aaaagcattt tcccgtgtgt agttatcatc ccggcaaacg
    40921 tcccacaccg aaaagttaac actgtccgaa atatgacttt ttcaaactga aattacaatc
    40981 gtaattaatt cacccctaag tattttttgg catgtaagtt gctttattta ctgtgtaagc
    41041 aatgaaatca gccgcaacgc aaacgggttg caccaccaca aatcagcggt agcaccaaaa
    41101 aggtgcggtt taagaatttt gcttcggaat ggtgcggcgc acctcgcaaa aataaacaat
    41161 aaaacagcaa tatctttaca tttatccaaa ctaaatgtta cctaacaaac acaacactgc
    41221 acaataaagt cgcagatgat aacaacacaa acactcacaa cgggtatcca tgcgttttta
    41281 acgcagaaga taaaggagtt ggatatgcaa ttacgtaaac ctgccacagc aatcctggcc
    41341 ctggcgcttt ccgcaggact ggcacaggca gatgacgccg ccccggcagc aggcagcacg
    41401 ctggacaaaa tcgccaaaaa cggcgtgatt gtcgtcggtc accgtgaatc ttcagtacct
    41461 ttctcttatt acgataatca gcaaaaagtg gtgggctact cgcaggatta ctccaacgcc
    41521 attgttgaag cagtgaaaaa gaaactcaac aaaccagact tacaggtgaa attgattccg
    41581 atcacctcac aaaaccgtat tccactgctg caaaacggca ctttcgattt tgaatgcggt
    41641 tctaccacca acaatgccga acgccaaaaa caggcggctt tctctgacac tattttcgtg
    41701 gtcggtacgc gcctgctgac caaaaagggc ggcgatatca aagattttgc cgacctgaaa
    41761 ggcaaagccg tagtcgtcac ttccggcacc acctctgaag ttttgctcaa caaactgaat
    41821 gaagagcaaa aaatgaatat gcgaattatc agcgccaaag atcacggtga ctctttccgt
    41881 accctggaaa gcggtcgtgc cgttgccttt atgatggatg atgctctgct ggctggtgaa
    41941 cgtgcgaaag cgaagaaacc agacaactgg gaaatcgtcg gcaagccgca gtctcaggaa
    42001 gcctacggtt gcatgttgcg taaagacgat ccgcagttca aaaagctgat ggatgacacc
    42061 atcgctcagg tgcagacctc cggtgaagcg gaaaaatggt ttgataagtg gttcaaaaat
    42121 ccaattccgc cgaaaaacct gaacatgaat ttcgaactgt cagacgaaat gaaagcactg
    42181 ttcaaagaac cgaatgacaa ggcactgaac taattacaag aaccaggggc ggaaattcca
    42241 gccctctcga ttgttacgta gcacggacag actatacgcc tggtggtcgt tccccatcgg
    42301 gcctgaaaac cgcaatacgc tgggtaacaa tcttcgaggg tagcagttaa cgctgctacc
    42361 ctcttttttc tggagtagat ttatgtctat agactggaac tggggtattt ttttacaaca
    42421 agccccgttc ggcaacacca cctatcttgg ttggatctgg agtggctttc aggtgacgat
    42481 cgctttgtcg atctgcgcct ggattatcgc tttcctcgtc ggttcatttt tcggcatttt
    42541 acgtaccgtt ccaaaccgtt ttctctccgg tctgggtacg ttgtatgtcg aactgttccg
    42601 caacgtgccg ttgattgtgc aattctttac ctggtacctg gtgatcccgg agctgctgcc
    42661 agagaaaatc ggcatgtggt ttaaggccga gctggatccc aatattcagt ttttcctttc
    42721 atccatgctt tgcctggggc tgtttaccgc cgcccgtgtt tgcgaacagg tacgcgccgc
    42781 tattcagtcg ctgccgcgtg ggcagaagaa tgccgcgctg gcgatgggtc tgacgctacc
    42841 gcaggcttac cgctacgtac tgctgcctaa tgcttatcgc gttatcgtcc cgccgatgac
    42901 ctcagagatg atgaacctgg tgaaaaactc cgccatcgcc tcgactatcg gtctggtgga
    42961 tatggcggcg caggcgggta aattgctcga ttactcggcc cacgcctggg agtcgtttac
    43021 cgccattacg ctggcgtatg tattgattaa cgcttttatc atgctggtga tgacgctggt
    43081 tgaacgtaaa gttcgcctgc ccggcaacat ggggggcaaa taatgtacga gtttgactgg
    43141 agttccattg ttccttccct gccatatctg ctcgacgggc tggtgatcac gctgaaaatc
    43201 accgtcacgg cagtggtgat tggtattttg tggggcacca tgctggcggt gatgcgcttg
    43261 tccagctttg cgcccgtagc gtggtttgcc aaagcctacg ttaacgtatt ccgctcgatt
    43321 cctttagtta tggttttgct gtggttttac ctgatcgtac cgggttttct gcaaaacgtg
    43381 ctgggattat caccaaaaaa tgatatccgt ttaatttcgg cgatggtggc gttttcgatg
    43441 tttgaagcgg cgtactattc agagattatc cgtgctggta ttcaaagtat ttctcgtggt
    43501 cagtcgagcg ccgcgctggc gttgggaatg actcactggc agtcgatgaa actgattatt
    43561 ctgccgcagg cgttccgcgc gatggtgccg ctgttgctca ctcagggcat cgtactgttt
    43621 caggatacct cactggtgta tgtgttatct ctggccgatt tcttccgtac cgcctcaacc
    43681 attggtgagc gtgatggtac gcaggttgag atgatcctgt ttgccggatt tgtttatttc
    43741 gttattagcc ttagcgcgtc gttgttggtc agctacttga aaagaaggac agcataatga
    43801 ttaccctgaa aaatgtttca aaatggtatg gtcactttca ggtgctgacc gactgctcaa
    43861 ccgaagtgaa aaaaggcgaa gttgtggtgg tttgcggtcc gtctggttcc ggcaaatcaa
    43921 cgctgattaa aaccgtcaac ggcctcgaac cggtgcagca aggtgaaatc accgtcgatg
    43981 gcatcgtggt taacgacaag aaaaccgatc tggcgaaact gcgttcccgc gtcgggatgg
    44041 tgtttcagca tttcgagctg ttcccccatc tgtcgattat cgaaaacctg accctggcgc
    44101 aggtgaaagt gcttaaacgc aataaagcgc cggctcgtga aaaagcattg aaattgctgg
    44161 agcgcgtcgg gctttccgca catgccaata agtttccggc gcagctttcc ggcggccagc
    44221 agcagcgtgt ggcgatcgct cgcgcattgt gcatggatcc tattgccatg ctgttcgacg
    44281 aaccaacgtc ggcgctggat ccggagatga tcaacgaagt gctggacgtg atggtcgaac
    44341 tggcgaacga aggaatgacg atgatggtgg tgacccacga aatgggcttt gcccgtaaag
    44401 tggcgaatcg ggtgatcttt atggacgagg gtaaaattgt tgaagactcg ccgaaagacg
    44461 ctttcttcga tgatccgaaa tcggaccgcg caaaagactt cctcgcaaaa atcctgcatt
    44521 aatcactctg gcgcgcatac tcaattgcgc gccatctcac gctttctcgt ttcgtccctc
    44581 gtatctgtta catcccggcg ttacccttgt cgcaaagaaa cacacaacaa ggagcaacaa
    44641 tggcactgcc aattctgtta gattgcgacc caggtcatga cgacgctatc gcaatcgttc
    44701 tcgccctcgc ctcaccagag cttgatgtca aagcaattac gtcctccgcc ggaaaccaga
    44761 caccagaaaa aaccttacgc aatgttctgc gtatgctgac cttgcttaat cgcaccgata
    44821 ttccggtagc aagcggtgcg gtaaaaccgt taatgcgtaa tttgattatt gcggacaacg
    44881 tgcacggcga aagcggcctg gacggcccgg cattgccgga accggcattt gcaccgcaaa
    44941 actgcacggc agtggagctg atggcgaaaa cgctgcgtga aagtgaggaa cctgtcacca
    45001 ttgtttctac cggaccgcaa actaacgttg ccttgctgct caatagccac ccggaactgc
    45061 atagcaaaat tgcccgtatc gtgattatgg gcggcgcaat ggggcttggt aactggacgc
    45121 ctgcggctga atttaatatt tacgttgacc cggaagcggc agaaattgtc ttccagtcag
    45181 gaattccggt ggtgatggcc ggtctggatg ttactcacaa agcacaaatc cacgttgaag
    45241 acactgagcg tttccgcgcg attggcaacc ctgtttcaac cattgttgcc gaactgctgg
    45301 atttcttcct cgaatatcac aaagacgaaa aatggggctt tgtcggcgca ccactgcatg
    45361 acccatgcac catcgcctgg ctgttgaaac cggagttgtt taccactgtt gagcgctggg
    45421 ttggcgttga aacacaaggg aaatataccc agggtatgac ggttgttgat tattattatc
    45481 tgactggcaa taaaccgaat gccaccgtaa tggtcgatgt tgatcgtcag ggctttgttg
    45541 atttactggc tgatcgtctg aaattttacg cttaatcact cgccggatga catcacgcca
    45601 tccggcacca ctcatttcat tacggctcga acggacgtct ctggaactga tcatgaccac
    45661 atttagggca tagcgtcagc acttccggtg tgtagatcgg gagatggaag tgacatttct
    45721 cgcagaccag atttcccagc cctaccactt ctccgctgtg ataaacccca tgatgattga
    45781 ggtcctggaa aacttcgcgc cattcaagct gcgttttatc ggtgatgtct gccagctcct
    45841 gccacaagct ttctttaatc acccgcataa agacgctgtc agactcttct ttcaggctct
    45901 cttcatagct catggcgaac tcttccaggt cacgtctgac agctcgcgtc agctcatcga
    45961 cctcggttcg cgttaactcc cctgttttta ttacgcgctc gcgcgcctgt tccaccagtg
    46021 cgtcgatatc acgttcgcca ttgcgcaggc gttcgctcag tgacgcgacc agttcacggt
    46081 aatgttgagc aaccttgttc atcgtttcat ctcccgggtc gttaactgac tataataata
    46141 gacgttcttt gttccttgct ttgctaatac gccaacaaac tctgtaaatt aatgtatgaa
    46201 taatccgggc atcatttcaa cgtcgagtgc gcgaaaggct gttttgacgc gggcgtttgg
    46261 gctatgctat gcggatctga aaaaccacat caacgctaca tttgtagccg tattgaaaac
    46321 aggaccactg gctgccatgc aagagcaata ccgcccggaa gagatagaat ccaaagtaca
    46381 gcttcactgg gatgagaagc gcacatttga agtaaccgaa gacgagagca aagagaagta
    46441 ttactgcctg tctatgcttc cctatccttc tggtcgacta cacatgggcc acgttcgcaa
    46501 ctacaccatc ggtgacgtga tcgcccgcta ccagcgcatg ctgggcaaaa acgttcttca
    46561 gccaatcggc tgggacgcat ttggtctgcc tgcggaaggc gcggcggtga aaaacaacac
    46621 cgcgccagca ccgtggacgt acgacaacat cgcgtatatg aaaaaccagc tcaaaatgct
    46681 gggctttggt tatgactgga gccgcgagct ggcgacctgt acgccggaat actaccgttg
    46741 ggaacagaaa ttcttcaccg agctgtataa aaaaggcctg gtatataaga agacttcagc
    46801 ggtcaactgg tgcccgaacg accagaccgt actggcgaac gaacaagtta tcgacggctg
    46861 ctgctggcgc tgcgatacca aagttgagcg taaagagatc ccgcagtggt ttatcaaaat
    46921 cactgcttac gctgacgagc tgctcaacga tctggataaa ctggatcact ggccagacac
    46981 cgttaaaaca atgcagcgta actggatcgg tcgttccgaa ggcgtggaaa tcaccttcaa
    47041 cgttaacgac tatgacaaca cgctgaccgt ttacactacc cgcccggaca cctttatggg
    47101 ttgtacctac ctggcggtgg ctgcgggtca tccgctggca cagaaagcgg cggaaaataa
    47161 tcctgaactg gccgccttta ttgacgaatg ccgtaacacc aaagttgccg aagctgaaat
    47221 ggcgacgatg gagaaaaaag gcgtcgatac tggctttaaa gcggttcacc cattaacggg
    47281 cgaagaaatt cccgtttggg cagcaaactt cgtattgatg gaatacggca cgggcgcagt
    47341 tatggcagtt ccgggtcacg accagcgcga ctacgagttt gcctctaaat acggcctgaa
    47401 catcaaaccg gttatcctgg cagctgacgg ctctgagcca gatctttctc agcaagccct
    47461 gactgaaaaa ggcgtgctgt tcaactctgg cgagtttaat ggtcttgacc atgaagcggc
    47521 cttcaacgcc atcgccgata aactgactgc gatgggcgtt ggcgagcgta aagtgaacta
    47581 ccgcctgcgc gactggggtg tttctcgtca gcgttactgg ggcgcgccga ttccgatggt
    47641 gacgctggaa gacggtaccg taatgccgac cccggacgac cagctgccgg tgatcctgcc
    47701 ggaagatgtg gtgatggacg gcattaccag cccgattaaa gcagatccgg aatgggcaaa
    47761 aactaccgtt aacggtatgc cagcactgcg tgaaaccgac actttcgaca cctttatgga
    47821 gtcctcctgg tactatgcgc gctacacttg cccggagtac aaagaaggta tgctggattc
    47881 caaagcggct aactactggc tgccggtgga tatttacatt ggtggtatcg aacacgccat
    47941 tatgcacctg ctctacttcc gcttcttcca caaactgatg cgtgatgcag gcatggtgaa
    48001 ctctgacgaa ccagcgaaac agttgctgtg tcagggtatg gtgctggcag atgccttcta
    48061 ctatgttggt gaaaacggcg aacgtaactg ggtttccccg gttgatgcta tcgttgaacg
    48121 tgacgagaaa ggccgtatcg tgaaagcgaa agatgcggca ggccatgaac tggtttatac
    48181 cggcatgagc aaaatgtcca agtcgaagaa caacggtatc gacccgcagg tgatggttga
    48241 acgttacggc gcggacaccg ttcgtctgtt tatgatgttt gcttctccgg ctgatatgac
    48301 tctcgaatgg caggagtccg gcgtggaagg ggctaaccgc ttcctgaaac gtgtctggaa
    48361 actggtgtac gagcacacag caaaaggtga tgttgcggca ctgaacgtcg atgcgctgac
    48421 tgaagatcag aaagcgctgc gtcgcgatgt gcataaaact atcgctaaag taaccgatga
    48481 tatcggccgt cgtcagacct tcaacaccgc aattgcggcg attatggagc tgatgaacaa
    48541 actggcgaaa gcaccgaccg atggcgagca ggatcgcgcc ctgatgcagg aagcgctgct
    48601 ggcggtagtc cgtatgctta acccgttcac cccgcacatc tgcttcacgc tgtggcagga
    48661 actgaaaggc gaaggcgata tcgacaacgc gccgtggccg gttgctgacg aaaaagcgat
    48721 ggtggaagac tccacgctgg tcgtggtgca ggttaacggt aaagtccgtg caaaaatcac
    48781 cgttccggtg gacgcaacgg aagaacaggt tcgcgaacgt gctggccagg aacatctggt
    48841 agcaaaatat cttgatggcg ttactgtacg taaagtgatt tacgtaccag gtaaactcct
    48901 caatctggtc gttggctaag cgcgggagga agcgtgcgat atctggcaac attgttgtta
    48961 tctctggcgg tgttaatcac cgccgggtgt ggctggcatc tgcgtgacac cacgcaggtt
    49021 ccttccacta tgaaagtcat gatcctggac tcaggcgatc cgaacgggcc attaagccgt
    49081 gcggtgcgta accagttacg tctgaatggt gtcgagttgc ttgataaaga aaccacgcgt
    49141 aaggacgttc catccttgcg tttgggtaaa gtgagcatcg cgaaagatac cgcatcggta
    49201 ttccgtaacg gtcaaacagc agagtatcag atgatcatga cggtgaatgc gaccgtgttg
    49261 atccccggcc gtgacatcta cccgattagc gccaaagtct tccgttcgtt cttcgataac
    49321 ccgcaaatgg cgttagcgaa agataacgaa caagacatga tcgtaaaaga gatgtacgac
    49381 cgtgctgccg aacagctgat tcgtaagctg ccaagcatcc gtgctgcgga tattcgttcc
    49441 gacgaagaac agacgtcgac cacaacggat actccggcaa cgcctgcacg cgtctccacc
    49501 acgctgggta actgatgatt cggttgtacc cggaacaact ccgcgcgcag ctcaatgaag
    49561 ggctgcgcgc ggcgtatctt ttacttggta acgatcctct gttattgcag gaaagccagg
    49621 acgctgttcg tcaggtcgct gcggctcaag ggttcgaaga acaccacact ttttccattg
    49681 atcccaacac cgactggaat gcgatttttt cgttatgcca ggccatgagc ctgtttgcca
    49741 gtcgacaaac gctattgctg ttgttacccg aaaacggacc gaatgcggcg atcaatgaac
    49801 aacttctcac actcaccgga cttctgcatg acgatctact gttgatcgtc cgcggtaata
    49861 aattaagcaa agcgcaagaa aatgccgcct ggtttactgc tcttgcgaat cgcagcgtgc
    49921 aggtgacctg tcagacaccg gagcaggctc agctgccccg ctgggttgct gcgcgcgcaa
    49981 aacagctcaa cttagaactg gatgacgctg caaatcaggt gctctgctac tgttatgaag
    50041 gtaacctgct ggcgctggcc caggcactgg agcgtttatc gctactctgg ccggacggca
    50101 aactgacatt accgcgcgtt gaacaggcgg tgaatgatgc cgcgcatttc accccttttc
    50161 attgggttga tgctttgttg atgggaaaaa gtaagcgcgc gttgcatatt cttcagcaac
    50221 tgcgtctgga aggcagcgaa ccggttattt tgttgcgcac attacaacgt gaactgttgt
    50281 tactggttaa cctgaaacgc cagtctgccc atacgccact gcgtgcgttg tttgataagc
    50341 atcgggtatg gcagaaccgc cggggcatga tgggcgaggc gttaaatcgc ttaagccagc
    50401 agcagttacg tcaggccgtg caactcctga cacgaacgga actcaccctc aaacaagatt
    50461 acggtcagtc agtgtgggca gagctggaag ggttatctct tctgttgtgc cataaacccc
    50521 tggcggacgt atttatcgac ggttgatatg aaatctttac aggctctgtt tggcggcacc
    50581 tttgatccgg tgcactatgg ccatcttaaa cccgtggaaa cgctggcgaa tttgattggt
    50641 ctgacgcggg tcacaatcat ccctaataat gttcctccgc atcgtcccca gccggaagcg
    50701 aacagcgtgc agcgtaaaca catgcttgaa ctggcgattg ccgacaagcc attatttact
    50761 cttgatgaac gcgagctaaa gcgcaatgcc ccctcttaca ctgcgcaaac actgaaagag
    50821 tggcggcagg aacaagggcc agacgtgccg ctggcgttta ttattggtca ggattcattg
    50881 ctgacctttc cgacctggta cgaatacgaa acgatactcg acaatgcaca tttgatcgtc
    50941 tgtcggcgtc caggttaccc acttgaaatg gcacaaccgc aataccagca atggctggaa
    51001 gatcatttga cacataaccc ggaagatctt caccttcagc ctgccggtaa aatttatctg
    51061 gctgaaacgc cgtggtttaa catctcggcg accatcatcc gcgaacgttt gcaaaatggt
    51121 gaatcgtgtg aggatttatt accggaaccg gtactgactt acattaacca acaaggcttg
    51181 tatcgctgat acctgtcgtt acggagggca tgatgcgact gtggttaatt cgtcatggtg
    51241 aaacgcaagc gaatgtcgat ggtctgtaca gcggtcatgc gcccacaccc ctgaccgcgc
    51301 gcggtattga gcaagcgcaa aatctgcata cgctgctaca tgatgtttcc tttgacctgg
    51361 ttttatgcag tgaactggaa cgggcacagc ataccgcgcg actggttctt agtgaccgcc
    51421 agcaccccgt tcatatcatc cctgaactca atgaaatgtt ttttggcgac tgggagatgc
    51481 gacatcatcg tgacctcatg caagaagatg ccgaaaacta tagcgcgtgg tgcaatgact
    51541 ggcagcatgc tatccccacg aacggtgaag gatttcaggc attttcgcaa cgtgtggaac
    51601 gatttatcgc aaggcttagt gaatatcagc actatcagaa tattttagtc gtcagccatc
    51661 agggcgtact gagtctgtta attactcgtt taattggtat gcctgccgag tctatgtggc
    51721 atttccgcgt tgaccagggg tgttggagcg ccattgatat caaccaaaaa ttcgcgacgc
    51781 tacgcgtcct caatagccgt gccatcgggg tcgagaatgc atgacttttc tgttttttta
    51841 cgggtaagcc gcaacgacca ttgacagccc cgggcaggct gatattctcc gcagccagac
    51901 tttttccgcc agacacgact ttgtagaaat tgttttacaa aaatggcgat gcaatctgcg
    51961 gcgcggggtg ggatgatagc ccactttcga aagccgattc ggcgacaatt gtcccgaaat
    52021 cgcctctggt tcaggtatac tgacagacca tttttatcta tttgattcac ccagggggaa
    52081 aacttgcagg gtaaagcact ccaggatttt gttatcgaca aaattgatga cctcaaaggt
    52141 caggacatca tcgccttaga cgttcagggc aaatccagca tcaccgactg catgatcatc
    52201 tgtacgggta cgtccagccg ccatgttatg tccattgctg atcacgttgt gcaggagtct
    52261 cgcgcagcgg gcctgttacc gctcggcgta gaaggtgaaa acagcgccga ctggattgtc
    52321 gtggatttgg gcgatgtgat tgtccatgtc atgcaggaag agagccgtcg cctgtatgaa
    52381 ctggaaaaac tctggagtta atgcgtgaag ctgcaacttg tcgccgtggg aacgaaaatg
    52441 ccggactggg tacaaaccgg ttttaccgag tacctgcgtc gttttccgaa agatataccc
    52501 ttcgagctga ttgaaattcc ggccggaaaa cgcggcaaga atgcggacat caagcgcata
    52561 ctcgacaaag agggtgagca gatgttggcg gccgcaggca aaaaccgcat tgtcaccctc
    52621 gatattccag gcaagccctg ggatacgccg cagttagccg ctgagctgga acgctggaag
    52681 ctggatggtc gcgacgtcag tctactgatt ggcgggcctg aagggttgtc gcctgcctgt
    52741 aaagcggcgg cggagcagag ctggtcgctg tcggcactta cccttcccca tccgctggtt
    52801 cgcgtgctgg tcgcagagag tctgtaccgg gcgtggagca tcaccaccaa ccatccttat
    52861 caccgtgagt gataagtgag ctttgagtag aaaacgcagc ggatgaaact acagaactct
    52921 tttcgcgact atacggcaga gtccgcgctg tttgtgcgcc gggcgctggt cgcctttttg
    52981 gggattttgc tgctgaccgg cgtgcttatc gccaacctgt ataatctgca aattgttcgc
    53041 tttaccgact accagacccg ctctaatgaa aaccgcatta agctggtgcc tatcgcaccc
    53101 agccgcggca ttatctacga ccgtaacggt atccctctgg ctctcaaccg cactatctac
    53161 cagatagaaa tgatgccgga gaaagtcgat aacgtgcagc aaacgctgga cgctttgcgc
    53221 agcgtggttg atctgaccga tgatgatatt gctgcattcc gaaaagagcg cgcacgttca
    53281 caccgtttca cctctattcc ggtgaaaact aacctgaccg aagtacaagt agctcgcttt
    53341 gccgtcaacc agtaccgttt tcctggcgtc gaagttaaag gctataaacg tcgttactat
    53401 ccttacggtt cagcgttgac ccacgtcatc ggctatgtgt cgaaaatcaa cgataaagac
    53461 gtcgaacgcc tgaataatga cggcaaactg gccaactatg cggcaacgca tgatatcggt
    53521 aagctgggca ttgagcgtta ctatgaagac gtgctgcacg gtcagaccgg ttatgaagag
    53581 gttgaagtta acaaccgtgg gcgtgttatc cgccagttaa aagaagtacc accgcaagcc
    53641 ggacacgata tttacctgac gctggatctc aaactccagc aatatattga aacgctgctg
    53701 gcgggtagcc gcgcagcggt ggtagtcacc gatccgcgta caggtggggt gctggcgctg
    53761 gtttccacgc ctagttatga cccaaaccta tttgttgacg gtatctccag caaagattat
    53821 tccgccttgc tgaacgaccc gaatacaccg ctggtgaacc gcgccacaca gggggtttat
    53881 cctcccgcgt ctacagttaa accctatgtg gcggtttcgg cattgagcgc cggagtgatc
    53941 acgcgcaata cgacgctgtt tgaccctggc tggtggcaac tgccagggtc ggaaaaacgt
    54001 tatcgtgact ggaaaaaatg gggccacggg cgtctgaatg tcacaagatc gctggaagaa
    54061 tctgcggata ccttcttcta tcaggtggcc tacgatatgg gaatcgatcg cctctccgaa
    54121 tggatgggta aattcggtta tggtcattac accggtatcg acctggcgga agaacgttcc
    54181 ggcaacatgc ctacccgcga atggaaacag aaacgcttta aaaaaccgtg gtatcagggt
    54241 gacaccattc cggttggtat cggtcagggt tactggacag cgaccccaat ccagatgagt
    54301 aaggcactga tgatcctgat taatgacggt atcgtgaagg ttccgcattt gctgatgagc
    54361 accgccgaag acggcaaaca ggtgccatgg gtacaaccgc atgaaccgcc cgtcggcgat
    54421 attcattccg gttactggga gctggcaaaa gacggtatgt acggtgttgc taaccgccct
    54481 aacggtactg cgcataaata ctttgccagc gcaccgtaca aaattgcggc gaaatctggt
    54541 acagctcagg tcttcggtct gaaagcgaac gaaacctata atgcgcacaa aattgccgag
    54601 cgtttacgtg accacaaact gatgaccgcc tttgcgccat acaacaatcc gcaagtcgct
    54661 gtcgccatga ttctggagaa cggcggtgcg ggtccggcgg ttggtacgct gatgcgccag
    54721 atcctcgacc acattatgct gggtgataac aacaccgatc tgcctgcgga aaatccagcg
    54781 gttgccgcag cggaggacca ttaatcatga cggataatcc gaataaaaaa acattctggg
    54841 ataaagtcca tctcgatccc acaatgctgc tgatcttact ggcattgctg gtttacagcg
    54901 ccctggttat ctggagcgcc agcggtcagg atattggcat gatggagcgt aaaatcggcc
    54961 agattgcgat gggtctggtc atcatggtgg tgatggcgca aattcctcca cgcgtttatg
    55021 aaggctgggc cccctatctc tatatcatct gtattatttt gctggtggcg gtagatgctt
    55081 tcggtgccat ctctaaaggc gctcaacgct ggctggacct cggtattgtt cgttttcaac
    55141 cgtcggaaat tgccaagata gccgtaccgt tgatggttgc acgctttatc aaccgcgacg
    55201 tttgcccacc ttcattgaag aatacagcta tcgcgctggt gctgatattt atgcccacgc
    55261 tgctggtggc tgcacagcct gacttgggaa catcaatcct cgttgcgctt tccggcctgt
    55321 ttgtactgtt cctctctggc cttagctggc gtctgattgg cgtcgcagta gtgctggtag
    55381 cggcgttcat tccgattctg tggttcttcc tgatgcatga ttaccagcgc cagcgcgtaa
    55441 tgatgctcct ggacccggaa tcagacccac tcggcgcagg ctatcacatt attcagtcta
    55501 aaattgctat tggctccggc ggattacgcg gcaaaggctg gctgcacggc actcagtcac
    55561 agcttgaatt tctccccgaa cgccataccg actttatctt cgcggtactg gcggaagagc
    55621 tgggattagt gggcattttg attctgctcg ctctctacat tctgctgatc atgcgcgggc
    55681 tgtggatagc cgccagagcg caaaccacct ttggtcgcgt catggctggc ggcttaatgc
    55741 tgatattatt cgtttatgtc ttcgtaaata ttggtatggt aagcggtatt ctgccggttg
    55801 taggggttcc gctcccactg gtcagttatg gaggatcggc gctaattgtg ctgatggctg
    55861 ggttcgggat tgtaatgtca atccacaccc acaggaaaat gttgtcgaaa agcgtgtaag
    55921 aggtgcgcaa tgcgtaagca gtggctcggg atctgcatcg cggcaggaat gctcacggca
    55981 tgtacaagcg atgatggtca gcaacagacg gtaagtgtac cgcagcctgc ggtatgtaac
    56041 ggccccatag ttgaaattag cggggcagac ccgcgtttcg aaccactgaa cgcgacggca
    56101 aatcaggatt accagcgcga cggtaaaagc tacaaaatcg tgcaggatcc gtctcgattt
    56161 agccaggcgg gactggcggc aatctatgat gccgaacccg gcagtaacct gacggcctct
    56221 ggcgaagctt tcgatccgac acagttgacg gcggcccatc caacgcttcc aatccccagc
    56281 tacgccagaa tcactaacct ggctaacggg cgaatgatcg tggtgcgcat taatgatcgc
    56341 ggcccttatg gcaacgaccg cgtgatttcg ctttctcgcg cggcagctga ccgtcttaac
    56401 acgtcaaaca acaccaaagt tcgtatcgat ccgattattg tcgcccagga tggttcgctt
    56461 tctggtcctg gtatggcttg taccacagtc gccaaacaga cttacgccct gccagcacct
    56521 cccgatttaa gcggtggcgc gggaacaagt tcagtgcctg tcccgcaggg tgacattctt
    56581 ccggtcagta attcgacgct aaaaagcgaa gatccgaccg gcgcgccggt aaccagcagc
    56641 ggtttcctcg gcgcaccaac gaccttagcg cctggtgtac tggaaggcag cgaaccgacg
    56701 cctgcgccac agcccgttgt tacagctccg tcgacaacgc ctgcaacctc acctgcaatg
    56761 gtgacaccgc aagccgcctc gcaaagcgcc agcggcaact ttatggtgca agtcggggcc
    56821 gttagcgatc aggctcgtgc gcaacagtac caacagcaac tgggacagaa gttcggcgtc
    56881 cccggtcgcg taactcaaaa tggcgcggtc tggcggatcc agcttggccc attcgccagc
    56941 aaagccgaag ccagtacctt gcagcaacgt ttgcaaaccg aagcccaatt acagtcattt
    57001 attaccaccg cgcagtagcg taaagcaggc atctgatgtg tcaatttctg taaatgacgt
    57061 taacaaagtc acgcggaaag tcagatgcct gccggtagtg catttgctat agtagggcac
    57121 tttttttaat tccatcacgg atgtcgtagt tcagaccatg aataccattt tttccgctcg
    57181 tatcatgaag cgcctggcgc tcaccacggc tctttgcaca gcctttatct ctgctgcaca
    57241 tgccgatgac ctgaatatca aaaccatgat cccgggtgta ccgcagatcg acgcggagtc
    57301 ctacatcctg attgactata actccggcaa agtactcgcc gaacagaacg ctgatgtccg
    57361 ccgcgatcct gccagcctga ccaaaatgat gaccagttac gttatcggcc aggcaatgaa
    57421 agccggtaaa tttaaagaaa ctgatttagt cactatcggc aacgacgcat gggccaccgg
    57481 taacccggtg tttaaaggtt cttcgctgat gttcctcaaa ccgggcatgc aggttccggt
    57541 ttctcagctg atccgcggta ttaacctgca atcgggtaac gatgcttgtg tcgccatggc
    57601 cgattttgcc gctggtagcc aggacgcttt tgttggcttg atgaacagct acgttaacgc
    57661 cctgggcctg aaaaataccc acttccagac ggtacacggt ctggatgctg atggtcagta
    57721 cagctccgcg cgagatatgg cgctgatcgg ccaggcgttg atccgtgacg taccgaatga
    57781 atactcgatc tataaagaaa aagaatttac gtttaacggt attcgccagc taaaccgtaa
    57841 cggcctgtta tgggataaca gcctgaatgt cgacggcatc aaaaccggac acactgacaa
    57901 agcaggttac aaccttgttg cttctgcgac tgaaggccag atgcgcttga tctctgcggt
    57961 gatgggcgga cgtactttta aaggccgtga agccgaaagt aaaaaattac taacctgggg
    58021 cttccgtttc ttcgaaaccg ttaacccact gaaagtaggt aaagagttcg cctctgaacc
    58081 ggtttggttt ggtgattctg atcgcgcttc gttaggggtt gataaagacg tgtacctgac
    58141 cattccgcgt ggtcgcatga aagatctgaa agccagctat gtgctgaaca gcagtgaatt
    58201 gcatgcgccg ctgcaaaaga atcaggtcgt cggaactatc aacttccagc ttgatggcaa
    58261 aacgatcgaa caacgcccgc tggttgtgct gcaagaaatc ccggaaggta acttcttcgg
    58321 caaaatcatt gattacatta aattaatgtt ccatcactgg tttggttaaa aattgaacac
    58381 ttgaaagtgt aatttccgtc cccatatact aagcatcagt aaaaaactcc cgccttctgg
    58441 cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa cttaacgaac
    58501 tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta cctgagctgg
    58561 ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc ccaacggtaa
    58621 aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc actcatatcg
    58681 agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc atggttctgt
    58741 aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc gctgtgatat
    58801 acttttcccc cacttttact cattctccac ggagacgccg ttttgtatca ggataaaatt
    58861 cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat gcatgaattc
    58921 accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca ctatccggta
    58981 ttcacccaag ggcaggcagg aaaggcggag catattttaa tgccgggtga tattccgttg
    59041 atccagagcg atcgcggtgg gcaggtgact tatcacgggc cggggcaaca ggtgatgtat
    59101 gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac cttgcttgag
    59161 caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg ggctgacgcg
    59221 cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgtat tcgacgcggt
    59281 tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt tttacgtatt
    59341 aatccttgtg ggtatgcagg aatggaaatg gctaaaatat cacaatggaa acccgaagcg
    59401 acgactaata atattgcacc acgtttactg gaaaatattt tagcgctact aaacaatcct
    59461 gacttcgaat atattaccgc ttaattccat acatcaatgg cccaatttac actgggtcat
    59521 tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt tctttatcac
    59581 ttgcagaaat gcgatgctac gccttgctcc gtagcccacc taattgatta actcattgaa
    59641 ataattgacc atggtgagca aaagcgttcc gtcccaatta gacataagat tattgtaatt
    59701 tttttaaata ttattttttc atgaatgaaa ttggagtaag tgcgtggata gtaataatca
    59761 aattgaaccc tgtttaagca gaaaatcgtc agaaggtaaa ccacaaatat ttacaacctt
    59821 acgaaatatt gatcttaccc ttctgactat tttgaagttg catatgtcca taaaggaatc
    59881 gttaatgcag cgaaagtgct taatctgccc cccctcggtc ggagccctgg tccttcctgt
    59941 catctatcgg gcgattaaaa ctcactatcc acagctttta ctgcgcaacc cgcccatcgg
    60001 cggcgcggaa aaccaactca gtcagtttca aaccgatctc atcaccgata acatgttttg
    60061 cacaggacca tagatgttat ccgtaatgca ttttgaaaaa gtattgttac agccagatca
    60121 cagcgcagaa cgataaaatg gcacaatcca acaacaattt tacattttcg cgaccgcttt
    60181 ggctgctttc aggtccgttt caatgatata ctgccagtcg ttaattcaaa aatagttgat
    60241 aattacaaca atctattgaa ttgaaacgct ttccttcata attcgcaact ggaacacgca
    60301 cgctatgagt aaacccattg tgatggaacg cggtgttaaa taccgcgatg ccgataagat
    60361 ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc gcaagccgga
    60421 atggatgaaa atcaagcttc cagcggactc tacacgtatc cagggcatca aagccgcaat
    60481 gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc tggcggaatg
    60541 ctttaaccac ggcacagcaa cgtttatgat cctcggcgct atttgtaccc gccgttgccc
    60601 gttctgtgac gttgcccacg gtcgcccggt agctcctgat gccaatgaac cagtgaaact
    60661 ggcgcagacc attgccgata tggcgctgcg ttatgtggtt atcacctccg ttgaccgtga
    60721 tgacctacgc gatggcggtg cccagcactt tgcggattgc attactgcca ttcgggaaaa
    60781 aagcccgcaa atcaaaattg aaactctggt gccggatttc cgcggtcgta tggatcgtgc
    60841 tctggatatt ctgactgcaa cgccaccaga tgtgttcaac cataacctgg aaaacgtacc
    60901 gcgtatttac cgtcaggtac ggcctggcgc agattacaac tggtcgctta agctgctgga
    60961 acgctttaaa gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg tgggactggg
    61021 tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg gtgtgacgat
    61081 gctaacgctg gggcaatatt tgcagccaag ccgccatcac ctgccggttc aacgttacgt
    61141 tagcccggat gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct ttacccatgc
    61201 tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga aagggatgga
    61261 agttaagtaa tcctgtaata attacttaca tctgtctgat aaaaaacccg ctttttgaag
    61321 cgggtttttg tatcgaacaa gatattgagg gagcgtcctg ctcgccacgt cactctttat
    61381 gagagagctt ttcagcctga agatcaacgt ctgcgccttt tttcgccgca gcatcgtcat
    61441 cattcatcgc cttcttgaac cctttaatgg ccgctccaag gtctccgccc agcgtacgta
    61501 acttcttagt cccaaacagc agaacgacca gcgccgcaac taccagcagt ttggtaatac
    61561 taatctcacc catagatacc ttcttgacat ataaccggca acagaacgcg ccttaaaaac
    61621 cagagaatat taacggtcat tcgacgcgcg cacacaatag caatctgtaa caaggtgaat
    61681 caagcgccga gtaaaaaaac atcataataa ttgcggcggc gcaaagcgac ggttgtttaa
    61741 gacgggcagt tgcgcgcgta cctgacgcac acgttcgggc gtcacttccg ccataatgag
    61801 tgcaggcatt tctgacgctg ccgcaatggt gacgccaaag ggatctataa tccggctttg
    61861 cccgatattt ttgttcccgc actcccccgc cgccaccata taacaggtgg tatccagcgc
    61921 acgagcggca agcaacgttg accagtgatg ctctttgagc ggcccacgaa cccaggcggc
    61981 aggaagtacc aggatttcag ctccctgtaa tgcctgtgcc agcgccagct ctggaaagcg
    62041 taagtcataa caggtcatca gaccgacctt catcccttcc acctccagta acggagctat
    62101 ttcattacca gcatcaacat ggcgtgattc ctgaatggca aatgcatcat agagatgcag
    62161 tttggcataa cgggcgacga tgtttcctgc ctgtagcgcc accagcatat tccacgcgcg
    62221 ccccggtgtt gaaggaacat gaatcgtcag aattgtcgtc atcatgttac gtttactttc
    62281 tcgccgtaaa cggtcgagga attcgccttc cagcagctgt gctgatttaa ccgaaagatc
    62341 cgcatcatga tcatcacgcg ccagtaatgc ttccggcagg acaaacagcg aaacctcgtt
    62401 ttccgccgcc tgcgccatca acgaggcaca aatctcagcg ttcttttccc acacagatgt
    62461 aacagcaaac tgtcctgcgg caaccagcat tgccacctcc tgtcagagtc gtaaaattcg
    62521 ccaacggcga ttattcggcg ttacacttat cactcataca aatcaaatag caggattttg
    62581 cagtgttaca acttctttta gcagttttta ttggcggtgg tacgggaagc gtggcgagat
    62641 ggctgttaag tatgcgattt aacccgctgc atcaggcgat tccgttgggg acgctggcag
    62701 caaacctgat tggggcattc atcataggaa tgggattcgc ctggttcagc aggatgacga
    62761 acattgatcc agtgtggaaa gtattaatca ccaccggatt ttgtggcggt ctaacaacct
    62821 tctcaacatt ttcggcagaa gtggtgtttt tgttacaaga gggccgcttt ggctgggcgt
    62881 tactgaacgt tttcgtcaat cttttggggt cttttgccat gaccgcactg gctttctggc
    62941 tgttttcggc ctcaaccaca cactaaagac caaaaaaaac ccgctgatta agcgggtttt
    63001 gaattcttgc tgacgtatct tacagagcga ttacgtttgc agcagaaggg cctttggcac
    63061 cgttagtgat ttcgaactct acgcgctgac cttcagcaag agttttaaaa ccattagtct
    63121 ggattgcaga gaagtgtacg aacacgtctt tgctgccgtc ttccggagta atgaaaccga
    63181 atcctttgga ctcattaaac cacttaacgt tacctttaat cttagacatc aaaattacct
    63241 ttacatgaaa aatagacaca aatgctgtgt cggttaccag tacaccaatt gtggtacgct
    63301 ttgtccagtg caactttact aaaaagtgat aaaagtcgcg ttatttttga tgttaattat
    63361 tttagtggtt gattttaaag gatttactaa aacttcattt gtctcaaaac tgaaagagca
    63421 tccaggcaaa gtacacattg ccattgttgt aggtacccgg aatgtaggtc atctgaaacg
    63481 tcgcggggcc ataccccaca gaggccaatg gcagtagaac cggaagaggg atgtaattcc
    63541 agttatcgcg cgccgttacg ccagcggtaa atcccaggcc taaatgaaaa ttttcatccg
    63601 ccagcggtcg ccaggtactt tcccatccgt atccggcaat cggttcccat ttgttccacg
    63661 agtccttaaa tgccattgca tacaggccat gccagtttcc tttttcat
//
LOCUS       DAZGJT010000015        58724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZGJT010000015 DAZGJT010000000
VERSION     DAZGJT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.015"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(6..221)
                     /locus_tag="ACHKFK_001062"
     CDS             complement(6..221)
                     /locus_tag="ACHKFK_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO9051453.1"
                     /translation="MALNCPTCKTPLERNGETAHCATCDKDFTVQALCPECHQALQVL
                     KACGAVDYFCQHGHGLISKKRVEFIIA"
     gene            complement(284..1756)
                     /gene="der"
                     /locus_tag="ACHKFK_001063"
     CDS             complement(284..1756)
                     /gene="der"
                     /locus_tag="ACHKFK_001063"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO9051454.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYALGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDEFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKSK"
     gene            complement(1874..3052)
                     /gene="bamB"
                     /locus_tag="ACHKFK_001064"
     CDS             complement(1874..3052)
                     /gene="bamB"
                     /locus_tag="ACHKFK_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO9051455.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVNLAEKDG
                     WFSKDPALLSGGVTVSGGHVYIGTEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(3063..3683)
                     /gene="yfgM"
                     /locus_tag="ACHKFK_001065"
     CDS             complement(3063..3683)
                     /gene="yfgM"
                     /locus_tag="ACHKFK_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO9051456.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDAIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(3701..4975)
                     /gene="hisS"
                     /locus_tag="ACHKFK_001066"
     CDS             complement(3701..4975)
                     /gene="hisS"
                     /locus_tag="ACHKFK_001066"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO9051457.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(5086..6204)
                     /gene="ispG"
                     /locus_tag="ACHKFK_001067"
                     /gene_synonym="gcpE"
     CDS             complement(5086..6204)
                     /gene="ispG"
                     /locus_tag="ACHKFK_001067"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO9051458.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(6231..7238)
                     /gene="rodZ"
                     /locus_tag="ACHKFK_001068"
     CDS             complement(6231..7238)
                     /gene="rodZ"
                     /locus_tag="ACHKFK_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO9051459.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELNNNQSQ
                     SVPLDTSTTTDQAMATTPTSPVDTTATNTQTPAVTAPAPAVDPQQNAVVPPSQANVDT
                     AATPAPAATTTPDGAAPLPTDQAGVTTPAVDPNALVMNFTADCWLEVTDATGKKLFSG
                     MQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQSPA
                     Q"
     gene            complement(7523..8677)
                     /locus_tag="ACHKFK_001069"
     CDS             complement(7523..8677)
                     /locus_tag="ACHKFK_001069"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO9051460.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(8827..9258)
                     /gene="ndk"
                     /locus_tag="ACHKFK_001070"
     CDS             complement(8827..9258)
                     /gene="ndk"
                     /locus_tag="ACHKFK_001070"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO9051461.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGSIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(9407..11719)
                     /gene="pbpC"
                     /locus_tag="ACHKFK_001071"
     CDS             complement(9407..11719)
                     /gene="pbpC"
                     /locus_tag="ACHKFK_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO9051462.1"
                     /translation="MPRLLTKRGCWIMLAAAPFIIILAAWAADKLWPLPLQEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSRERVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDGALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSVNLPEDPRPDSVSRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(11720..16681)
                     /gene="yfhM"
                     /locus_tag="ACHKFK_001072"
     CDS             complement(11720..16681)
                     /gene="yfhM"
                     /locus_tag="ACHKFK_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO9051463.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     VSSAKLSAPERQKLAQQSAGKALTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDDEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPVKLDVIKPDGQILRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     YEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGGGAVRPDRVTLKLDKASYCPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPTKMRPNQPLTVKIKASNKNGEMPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSNSPAPVELAPGVRTTLFIPVR
                     ALPGYGDGDIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAL
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKIMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDSALMLSLLEENKLLPDEQYSLLNTLSQQAFGERWLSTQESNALFLAAR
                     TLQDLPGKWQAQTTFSAEPLTGEKAQTSNLNSDQLATLQVTNSGDQPLWLRVDASGYP
                     QSAPLPASNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAADDLLIVRP"
     gene            16888..17733
                     /gene="sseA"
                     /locus_tag="ACHKFK_001073"
     CDS             16888..17733
                     /gene="sseA"
                     /locus_tag="ACHKFK_001073"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO9051464.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPATRFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVTNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            18069..18194
                     /gene="timP"
                     /locus_tag="ACHKFK_001074"
     CDS             18069..18194
                     /gene="timP"
                     /locus_tag="ACHKFK_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO9051465.1"
                     /translation="MKIRCFCIVLIVSGALLTEVNNNRSLSGNNLLVVNNLQNSK"
     gene            complement(18226..19002)
                     /gene="sseB"
                     /locus_tag="ACHKFK_001075"
     CDS             complement(18226..19002)
                     /gene="sseB"
                     /locus_tag="ACHKFK_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO9051466.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEGEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEVLEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAAGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(19145..20428)
                     /gene="pepB"
                     /locus_tag="ACHKFK_001076"
     CDS             complement(19145..20428)
                     /gene="pepB"
                     /locus_tag="ACHKFK_001076"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO9051467.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGIRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(20487..20687)
                     /gene="iscX"
                     /locus_tag="ACHKFK_001077"
     CDS             complement(20487..20687)
                     /gene="iscX"
                     /locus_tag="ACHKFK_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO9051468.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(20699..21034)
                     /gene="fdx"
                     /locus_tag="ACHKFK_001078"
     CDS             complement(20699..21034)
                     /gene="fdx"
                     /locus_tag="ACHKFK_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO9051469.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDVALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(21036..22886)
                     /gene="hscA"
                     /locus_tag="ACHKFK_001079"
     CDS             complement(21036..22886)
                     /gene="hscA"
                     /locus_tag="ACHKFK_001079"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO9051470.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDAVSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQAIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(22903..23418)
                     /gene="hscB"
                     /locus_tag="ACHKFK_001080"
     CDS             complement(22903..23418)
                     /gene="hscB"
                     /locus_tag="ACHKFK_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO9051471.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(23514..23837)
                     /gene="iscA"
                     /locus_tag="ACHKFK_001081"
     CDS             complement(23514..23837)
                     /gene="iscA"
                     /locus_tag="ACHKFK_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO9051472.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(23854..24240)
                     /gene="iscU"
                     /locus_tag="ACHKFK_001082"
     CDS             complement(23854..24240)
                     /gene="iscU"
                     /locus_tag="ACHKFK_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO9051473.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(24268..25482)
                     /gene="iscS"
                     /locus_tag="ACHKFK_001083"
     CDS             complement(24268..25482)
                     /gene="iscS"
                     /locus_tag="ACHKFK_001083"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO9051474.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(25594..26082)
                     /gene="iscR"
                     /locus_tag="ACHKFK_001084"
     CDS             complement(25594..26082)
                     /gene="iscR"
                     /locus_tag="ACHKFK_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO9051475.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(26353..27093)
                     /gene="trmJ"
                     /locus_tag="ACHKFK_001085"
     CDS             complement(26353..27093)
                     /gene="trmJ"
                     /locus_tag="ACHKFK_001085"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO9051476.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            27212..28015
                     /gene="suhB"
                     /locus_tag="ACHKFK_001086"
     CDS             27212..28015
                     /gene="suhB"
                     /locus_tag="ACHKFK_001086"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO9051477.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            28160..29014
                     /locus_tag="ACHKFK_001087"
     CDS             28160..29014
                     /locus_tag="ACHKFK_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9051478.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            29205..30485
                     /gene="csiE"
                     /locus_tag="ACHKFK_001088"
                     /pseudo
     CDS             29205..30485
                     /gene="csiE"
                     /locus_tag="ACHKFK_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
     gene            complement(30477..31616)
                     /locus_tag="ACHKFK_001089"
     CDS             complement(30477..31616)
                     /locus_tag="ACHKFK_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO9051479.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGVGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVSMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            31985..32407
                     /locus_tag="ACHKFK_001090"
     CDS             31985..32407
                     /locus_tag="ACHKFK_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO9051480.1"
                     /translation="MNSLRYFDFGTARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAVIAVVMEVPAAILIILGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(32567..33439)
                     /locus_tag="ACHKFK_001091"
     CDS             complement(32567..33439)
                     /locus_tag="ACHKFK_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO9051481.1"
                     /translation="MTIYTLSQGALKLEVSDQGGVIEGFWRDAAPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDAWLGQWQCVSRSEDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLAVTNKGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWREQEQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTISEMSLRVALL"
     gene            complement(33450..34544)
                     /locus_tag="ACHKFK_001092"
     CDS             complement(33450..34544)
                     /locus_tag="ACHKFK_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9051482.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGEGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(34577..35575)
                     /locus_tag="ACHKFK_001093"
     CDS             complement(34577..35575)
                     /locus_tag="ACHKFK_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9051483.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFDV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPIN
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(35600..37111)
                     /locus_tag="ACHKFK_001094"
     CDS             complement(35600..37111)
                     /locus_tag="ACHKFK_001094"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9051484.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIMDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(37134..38117)
                     /locus_tag="ACHKFK_001095"
     CDS             complement(37134..38117)
                     /locus_tag="ACHKFK_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9051485.1"
                     /translation="MPKKMTRTRNLLLMATLLGSALFARASDKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKTHVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFTQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(38214..41495)
                     /locus_tag="ACHKFK_001096"
     CDS             complement(38214..41495)
                     /locus_tag="ACHKFK_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO9051486.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHDDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGFFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALTPATAIQGVLHGINPERLTIELSDADGNIVLSYHENQPQALPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRASPTNQEVLCLHNLLLVLSGRQDNARL
                     QREKLLRDYPLNATLWWLNWFDGRSESALVQWRGLCQGRDVNALMTAVQLINWGMPAL
                     AADMLNALDCQRTLPLYLQASLLPKAERGELVVKAIDAFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYGKAIALWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGVTPEKRLARLGNNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAVCANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQHTAQQLFSEMKQWAKEMAKTSIEADFFAVSQPDLL
                     SLYSDLQQQHKEKCLMVAMLAAAALGEVAHYESARAELMAINPAWPKAALFTTVMPFI
                     FSYVH"
     gene            41613..42806
                     /locus_tag="ACHKFK_001097"
     CDS             41613..42806
                     /locus_tag="ACHKFK_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9051487.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFERFQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWATPIEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(42870..44123)
                     /gene="glyA"
                     /locus_tag="ACHKFK_001098"
     CDS             complement(42870..44123)
                     /gene="glyA"
                     /locus_tag="ACHKFK_001098"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO9051488.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            44452..45642
                     /gene="hmpA"
                     /locus_tag="ACHKFK_001099"
     CDS             44452..45642
                     /gene="hmpA"
                     /locus_tag="ACHKFK_001099"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO9051489.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPNEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(45687..46025)
                     /gene="glnB"
                     /locus_tag="ACHKFK_001100"
     CDS             complement(45687..46025)
                     /gene="glnB"
                     /locus_tag="ACHKFK_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO9051490.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(46086..47420)
                     /gene="glrR"
                     /locus_tag="ACHKFK_001101"
     CDS             complement(46086..47420)
                     /gene="glrR"
                     /locus_tag="ACHKFK_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO9051491.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGVEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMTRGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(47410..48123)
                     /gene="qseG"
                     /locus_tag="ACHKFK_001102"
     CDS             complement(47410..48123)
                     /gene="qseG"
                     /locus_tag="ACHKFK_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO9051492.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGNWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTDEVTPDEP"
     gene            complement(48288..49715)
                     /gene="qseE"
                     /locus_tag="ACHKFK_001103"
     CDS             complement(48288..49715)
                     /gene="qseE"
                     /locus_tag="ACHKFK_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO9051493.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAKPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(50291..54178)
                     /gene="purL"
                     /locus_tag="ACHKFK_001104"
                     /gene_synonym="purI"
     CDS             complement(50291..54178)
                     /gene="purL"
                     /locus_tag="ACHKFK_001104"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO9051494.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHTIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLVDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVRRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFSEELGAVIQVRAADREAVEAVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEGFHVL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWIRIFRNARKQLG"
     gene            54436..55992
                     /gene="mltF"
                     /locus_tag="ACHKFK_001105"
     CDS             54436..55992
                     /gene="mltF"
                     /locus_tag="ACHKFK_001105"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO9051495.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKYPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(55989..56492)
                     /gene="tadA"
                     /locus_tag="ACHKFK_001106"
     CDS             complement(55989..56492)
                     /gene="tadA"
                     /locus_tag="ACHKFK_001106"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO9051496.1"
                     /translation="MSEVEFSHEYWMRHAMTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(56550..57185)
                     /gene="yfhb"
                     /locus_tag="ACHKFK_001107"
     CDS             complement(56550..57185)
                     /gene="yfhb"
                     /locus_tag="ACHKFK_001107"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO9051497.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHNEARLQALQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            57394..58242
                     /locus_tag="ACHKFK_001108"
     CDS             57394..58242
                     /locus_tag="ACHKFK_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9051498.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNTEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            58298..58558
                     /gene="yfhL"
                     /locus_tag="ACHKFK_001109"
     CDS             58298..58558
                     /gene="yfhL"
                     /locus_tag="ACHKFK_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO9051499.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGEHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPQHVETEEQLWDKFVLMHHADKI"
BASE COUNT        13912 a        16351 c        14609 g        13852 t
ORIGIN      
        1 tgatattaag caataataaa ttcaacgcgt tttttagata ttaaaccgtg cccatgttgg
       61 cagaaataat ccacagcacc acaagctttt aacacttgta acgcctgatg acactcaggg
      121 caaagtgcct ggacggtaaa gtctttgtcg caagttgcac agtgagcagt ctcgccattg
      181 cgttcgagag gggttttgca ggttggacag ttgagtgcca taaatactcc tcgcgtgtta
      241 ttcggcttcc ctctccgtgt ggaaagggaa accagataag tgattactta cttttcttga
      301 tatgcttcat cagacgctta cgtttacgca tctgggttgg cgtcagggtg ttacgcttgt
      361 tcgcatacgg gttttcccct tctttgaact gaatacgaat cggcgatccc attacgtcca
      421 gcgatttgcg gaagtagttc atcaggtaac gcttgtagga atcaggcagg tctttcacct
      481 gattgccgtg aatcaccaca atcggcgggt tataaccccc ggcgtgggcg tatttcagct
      541 tcacacgacg accgcgtacc agcggtggtt ggtgatcttc aacagccatc gtcatgatgc
      601 gcgtcagcat agaggtcccc acacgacggg tggagctgtc atacgcttca cgtactgatt
      661 caaacaagtt accaacacca ctgccgtgca aggcagagat aaagtgcaca cgagcaaaat
      721 cgataaagcc cagacggaag tccagcgtct ctttcacctg ctctttcact tcctggctca
      781 ggccgtccca cttgttcact acaatcacaa gtgagcgccc actattgaga ataaagccca
      841 gcaaggagag atcctgatcg gaaatacctt cacgcgcatc aatcaccaac atcaccacgt
      901 tggcatcttc aatggcctgc aacgttttga ttaccgagaa tttctctacc gcatcggtga
      961 ttttgccgcg tttacgtacg ccagcggtgt caatgagcac atactcacgt ccgtcgcgtt
     1021 ccattggaat gtagatactg tcacgcgtcg tgccaggcat gtcgtaaaca acaacgcgct
     1081 cttcaccaag aatacggtta gtgagtgtag acttacctac gttcgggcga cccacaatcg
     1141 ccagtttgat cggcagactt tgcgggtcga actcgtcttc ctcttcttct tcgccgttct
     1201 cttccgcttc aaattgcgcc cagtattcgg cgtcttcgtc gacttcttct tgcggtgcga
     1261 gatcttccat ccacggcagc agcacgtgct ccagcagact taatacgcca cgaccgtgag
     1321 acgcggcgat cgggtagatt tcacctaaac caagcgcgta gaaatcaacc actgcctgat
     1381 cgggatccag accgtcagtt ttgtttgcca ccaggaaggt cggtttttca cgggagcgca
     1441 gatgtttagc aatcgcttca tccgccggca tcaggcccgc gcgcgcatcc accataaaca
     1501 gtacgacgtc cgcttcttca atcgccagca gcgactgttc cgccatgcgg gtttctacac
     1561 cgtcttctgt gccatcaatc ccgccggtat caatgcagat aaactcacgg ccttcaattt
     1621 ccgcacgacc gtacttacgg tcacgagtca gacccgggaa atccgcaacc agcgcatctc
     1681 gggtgcgagt tagacggtta aataacgtgg attttcctac gttagggcgc ccgacaagcg
     1741 cgaccacagg taccatgttt aaagcctcat ttttataaat catcagacaa cgcacgctat
     1801 attcgtgtcg ttgttaaaaa caggaaaacg gcccctgtcc aggagccgtt ttcaaagtga
     1861 acgacagaga cgattaacgt gtaatagagt acacggttcc gtcttttgcc tggatcagca
     1921 gtttgccgtc agcagcaacc ggttcagtct ggaaaccgga actatcaact ttttgctggg
     1981 caacgaaacg accatcttcg acgttaatcc agtgcagata accttcactg tcacctacca
     2041 ccaggttgcc attatacagc accggagaag tcagcaggcg atgcagcaga tcgctttgtg
     2101 tccacagcgt aacgccgcca tcaatggtca acgccatcac ccggtcattt tgatcgacca
     2161 gatagatgcg gttgccgtcg acgatgaaat cattcaccga acccagttcg cgtttccaca
     2221 taatctgacc gctgcgcaga tcaagcgccg tcaggttacc attataggcc agcgcgaaaa
     2281 caacgccgtt aacaacgacg ggagtcgtgt caacatcgct cagacggtca atttcggtag
     2341 aaccggtcgc ctgggaaata cgctgctgcc aaatcatctg gccctgttcc atcagcactg
     2401 cgctgacgcg accattatcg ccacccacga cggccgcacc aaaagccgtt gccggcgcag
     2461 actcgccacg caaagagagc gaaggcatat cgaggttaac tgtccatttg acagcgccgt
     2521 cagcttcgtt cagcgcttgt aactgaccgt tactggtgtg gattaacacc agaccgtcgc
     2581 tgaccaccgg gcgcgaaagt gcttcacccg caactttagt ttgccatgcc acagtaccat
     2641 cgctggtatt cagcgcgtaa acctgcgcct tttcggtgcc aatgtagaca tgcccaccag
     2701 acacggtcac gccgccagaa agtaatgcag gatctttaga gaaccagcca tctttctcgg
     2761 ccaggttgac agaccagatt tctttgccgt catccgcatt cagcgctttt actaaaccag
     2821 cgcggtcagc tgcatagaca acgttgtccg ccagtgccgg atgaagattg gaatagaagt
     2881 tgccaatgcc gctaccaacg gaagtgctcc acgcagtagt cggcgtaaac tggttttcaa
     2941 cggttggcaa tggggacatc tttaccacat cttcttcgct gttaaacagc gaacagccgc
     3001 ttaaaagggt aacggaaagc agtcctggca gcagtaattt acgcaattgc atcgggtccc
     3061 tctcagatgg acaaattatt aattttcatc tgcatcattt cgctcagtgc cggggtaaca
     3121 tcgcttttca cgcctgcttc ccatgcacta cgcgcacctt gcttatcacc tttgctcagc
     3181 aatgcttcac cacgcaggtc ggcaacaatg gcagcccacc cttcaccttt gatagcatca
     3241 agggttttta gcgcggcatc agcctgcttg agctgtacct gaacgcgagc aagacgcaga
     3301 tttatcacgg ctttgagatt ttcatcgctc gtgtctgcca gcccctgttg taactgggcg
     3361 gcagctttct ctagttcatt tttgtcaaca aattgctgcg caagttccaa agaagccagc
     3421 gcaccataag tatttttatt ttcagcagca aatttttccg ccgctgggat gctatccggt
     3481 ttgccttcgc tcactgcggt aaccgcattt tgataggcaa gagaagcgga gcgtgcagaa
     3541 tcaacctgat ggctgttcca gtagcgccag ccaatcagtg cgccaacgcc caaaatcacc
     3601 ccaacagcca gtgctttgcc attttcagca aaaaagcgtt taaccgcttc aacctggtcg
     3661 ttttcgttct cgtaaatttc cacgctgtcc ttctccttcc ttaacccagt aacgtgcgca
     3721 aatgcgcggc tacgctatcc tgcgcaactg cagtttgctc accagagcgc aaatccttca
     3781 ctactgctgt gccgttagcc acttcagact cacccagcac cacagcaacg cgggcacccc
     3841 atttatcagc acgggcaaac tgtttcttaa agttgccgcc gccgtggttg gtcatcagtt
     3901 tcacgcccgg taattcatca cgcagacgct ctgctaatgc catagccgca gattgtgtgt
     3961 cagcacctga agccaccagg tatatatcga caacaggatc ggctttaaat tccggattaa
     4021 cggcctgaac taacaataca agacgttcga ggcccatcgc aaaaccgact gccggtgttg
     4081 cacgaccacc cagttgttcc accagaccgt cataacgacc acctgcacac acggtgccct
     4141 gggaaccgag gctgttagtc acccactcga aaacggtacg gttatagtaa tccagaccgc
     4201 gcaccagacg ctggtttacg gtgtaagcga tccccgcgct ctcaagcagt ttgcacagac
     4261 cggcaaagtg ctcacgagat tcctcgtcca gataatcgcc taatgccgga gcgtcgttga
     4321 gaagcgcctg cacttccgga ttttttgaat ccagtacgcg caacgggtta gtgtacatgc
     4381 ggcgtttgca gtcttcgtcc agcttttctt tatgctgctc aaggaatgcc accagcgcat
     4441 cgcggtaatt cgcacgagct tccagcgaac cgatagagtt cagctcaaga gttacgtgct
     4501 cggaaatacc cagcgcgcgc caccagcggg cagtgagcat aatcagttcg gcgtcgatat
     4561 ccggaccttg cagaccgaaa acttcgcagc ccaactgatg gaactgacga taacgccctt
     4621 tctgcggacg ctcgtgacgg aacataggcc cgatatacca cagacgctgt tcctgattgt
     4681 acagaagacc atgctcgatg ccggcgcgta cacagcccgc cgtcccttca gggcgcagag
     4741 tcaggctgtc gccattgcga tcctcaaagg tgtacatctc tttttcaacc acgtcggtga
     4801 cttcaccaat cgcacgtttg aatagcgggg tctgctctac aatcggcaag cggatttcac
     4861 tgtaaccgta gctgccgagc acgtttttca gtgtgccttc aatgcgctgc cagatggccg
     4921 tttcgccagg caggtaatcg ttcatgccgc gaatggcttg aatgtttttt gccacgttta
     4981 ttctctttct gaatataaaa atgaaccctc aacgcttccc tcaatgtttc gggagccatg
     5041 cgggttcaat catacacggg aagcgaggcg cttcccatca cgttattatt tttcaacctg
     5101 ctgaacgtca attcgacgcg cttcgtccag ctgactggct ttcgcacgaa tgcgcgcttc
     5161 cagctgatcg atcatatcgt tgttgtccag acggtctttg cgcacgccat cttcatagag
     5221 gccgcttttc ttgttgccgc cagtgacgcc gagtgtagaa accagcgcct caccagggcc
     5281 attcaccacg cagccgataa tcgaaacgtc catcggagtg atgatatctt ccaggcgttg
     5341 ctccagcgcg ttaaccgtac cgataacatc aaattcctga cgcgaacagg tcgggcaggc
     5401 gatgaagttg atcccgcgcg aacggatacg cagcgatttc aaaatatcga aaccgacttt
     5461 gatctcttcg accggatcgg ccgccagcga tacgcgcagc gtgtcgccga tgccttcaga
     5521 cagcagcaga cctaaaccaa tagcggattt tactgccccg ctacgcgcac caccggcttc
     5581 ggtgatcccc agatgcagcg gctgatcgat ttgttttgcc agcaaacgat aagactcaac
     5641 agcgaggaag acgtcagacg ctttcacgct gactttgaac tgatcgaagt tcaggcgatc
     5701 gagatgatca acatgacgca tggcagattc cagcaacgcc tgcggcgtcg gttcgccata
     5761 cttttcttgc agatcttttt ccaacgatcc ggcattaacg ccaatacgga tcggaatgtt
     5821 tttatcgcgc gcacagtcaa ccaccatgcg aatacgctct tcattaccga tattgccagg
     5881 gttaatacgc agacaatcga cgccgtattc cgctactttc agcgcaatgc gatagtcgaa
     5941 gtggatgtca gccaccagcg gcacgttaac ctgctgtttg atgagtttga acgcttctgc
     6001 cgcgtccatc gtcggtacgg agacacggac gatatcagcg ccaacacgtt ccagcgcctt
     6061 gatttgattg accgttgctt cgacgtctgt cgtacgcgta ttggtcatgg actgtacggc
     6121 gatgggagca ccatcgccaa tcggcacatt cccaacgtaa atacgtgttg attttctacg
     6181 ttgaattgga gcctggttat gcatgaaaaa tctcccgcgt tacccgtctg ttactgcgcc
     6241 ggtgattgtt cggcattgag ggtcagacgc gcaacctggt tagttctgat aaaacgactc
     6301 agatcgacag gtttcccttg atactggatc tgtactgcgg ctggcgcacc aattttcagt
     6361 ttgtacggtg cctggccggt taagttcaaa ttaccgtctt tacgctgcat accgctaaac
     6421 agttttttac cggtagcatc ggtgacttcc agccagcaat cggcagtaaa gttcatcacc
     6481 agcgcattcg ggtcaaccgc cggcgtggtc acgccagcct gatcggttgg caacggcgca
     6541 gcgccatctg gtgttgttgt tgccgctggt gccggagtcg cagcggtatc aacatttgcc
     6601 tgcgaaggtg gaacaaccgc attctgttgc ggatcaacag ctggtgctgg cgcagttacg
     6661 gcaggtgttt gcgtgttggt tgcggtggta tccacaggtg atgtcggcgt ggtcgccata
     6721 gcctgatctg tggttgtcga cgtatctaac ggtacactct ggctttgatt attattcagt
     6781 tccgcagaag attgatcggc catagtggtg atctcttcct gctgagcttt gtggtcttgc
     6841 caccaccagg caccgctcag gccaataacc acaaacaaca ccagccaggt gaaggtcatc
     6901 agccagccgt cacgtttttt gcggcgttta ccgagggaaa aactctgcat cggcgcaact
     6961 tttgcagccc gtagtggagc ctgcttttcc agccctggca gcagttcttc ttctggaata
     7021 tgtaccagac gcgcataaga gcggatatat ccgcgcagga atgttgaagc aagatcggcg
     7081 ggtgccttat cttcttcaat gtcgcgtacc gtggaaacct tcaggcaaag tcgctcggca
     7141 acggcctgtt gactaagtcc tagttgttcg cgagcattac gcaggcgagc gccggtagta
     7201 agtgcttcat tttggtcgtg cgtggcttca gtattcattc gctgcaggta cgtttaaatg
     7261 agaattgaga tgccggtgaa ccatcatgcc cacccacacc gcgaaacatc cgttaagtta
     7321 accgttatca tacagtataa gaccgtctgt gtgctcgtga caaagcccac attcgctaag
     7381 gctaacttac tgttgcatcg ttacatactg ccttaaagtc agcaaaaacg caccgttaat
     7441 attgaccaga caattgcaac ttaatacttc attcataaaa gattacgcca cggtacataa
     7501 agtaaccgtg gcgtaatggc tatcagaccg ctttaatgtc gatggcttca ccctgcatcc
     7561 gcttgcgcag agtacgtttc gtacggtcga taacatcgcc cgccaactga ccacaagcag
     7621 catcgatatc atcaccacga gttttacgca caatagtggt gaaaccgtag ctcatcagca
     7681 cttttgagaa acggtcgata cggctgttcg aactgcgtcc atacggcgcg cccgggaacg
     7741 ggttccacgg gatcaggttg atcttacacg gcgtatcttt cagtagttcc gccagttggt
     7801 gcgcgtgttc agtgccgtcg ttaacgtggt caagcatcac gtattcaata gtgactcggc
     7861 cctgattggc gttggatttc tccagataac ggcgcaccgc cgcaaggaac gtttcgatat
     7921 tgtacttttt gttgatcggc acaatctcgt cacgaatttc gtcgttcggc gcgtgcaggg
     7981 agatagccag cgcaacgtcg atcatatcgc ccagtttatc cagcgccgga accacaccgg
     8041 aagtggaaag cgtgacgcga cgttttgaca ggccaaaacc gaaatcatca agcatgattt
     8101 ccatcgccgg aacgacgttg ttcaggttga gcagcggctc gcccataccc atcatcacta
     8161 cgttagtgat tgggcgctga ccggtgactt ttgctgcgcc gacaattttc gccgcacgcc
     8221 acacctggcc gataatttcc gatacccgca ggttgcggtt aaagccctgc tgggcggtgg
     8281 aacagaattt acactccagc gcacacccca cctgcgaaga gacgcagagc gtggcacggt
     8341 cgtcttccgg gatatatacc gtttcaacgc gctgatcgcc aacggcgatc gcccatttaa
     8401 tggtgccgtc agatgaacgc tgttcttcaa ccacttccgg tgcacggatt tccgccacct
     8461 ctttcagttt gccgcgcaac actttattga tgtcggtcat ctcatcaaag ttgtcgcagc
     8521 aatagtgata catccacttc atcacctgat cggcgcggaa gggtttttca cctaaatctt
     8581 taaaaaactc ccgcatctgc tgacggttga gatccagcag gttgattttt ccatctttcg
     8641 tggtgacgtt ttcaggtgtg actaattgtt cagacatatg ctattccggc ctcgttatta
     8701 cacgttatgg cccctggagg gttgaaaaaa gaaacgcccc ggtgagcggc tgctcgtccg
     8761 ggggcgctgc attgtacaaa ttctggcgca cggatgccac gtttgcacgc ggcatttacg
     8821 aaattattaa cgggtgcgcg ggcacacttc gccttcgccg aagaaataag cgatttcgcg
     8881 agcggcagat tcgacggaat cagaaccgtg ggtaccattt tcggtcaggc tgtcagcgta
     8941 atcagcacgc agagtaccgg ccagtgcgtt tgccggattg gtcgcgccca gcagatcgcg
     9001 gtgacgctga acagcatttt caccttccag cacggaaaca acgatcgggc cagaagtcat
     9061 gaattcaacc agaccatcaa agaacggttt tccatcgtgt tcagcataaa agccacgagc
     9121 ctgttcaacg gtcaggtgca gcattttggt gccgacaatt ttgaaccctg cagcttcaaa
     9181 gcgcgcaaag atactaccaa tcacgttttt tgctaccgcg ttcggtttga tgatggaaaa
     9241 agtacgttca atagccattt ttacctctgt aaattgttct gttgttgtct gtaccagcgt
     9301 acgagatggc gcggattata atgagcaaca gggccgttga ctattgatga aggtaacatt
     9361 tttttaaaat aaacccagtt taagcaacaa tcagggacaa aacagactat tgcatgacaa
     9421 atttcactgt cgctatttgc cccacgtcat ccatcaccag caattgataa tcgcctttat
     9481 ccgtcaaatg cagggtcacg ttgcgcccac gttcagttaa cggttcgccg ttcagaaacc
     9541 accagcgttc acctgcccct ccacttgatt gcaacggcaa agtcgcttcc gctgcgcccg
     9601 gtaaacgttt aataatcgcg ccatcgcgca cacctgttag ctgcagcggg agttgtgcat
     9661 cgtgaccgta tggcgggcaa attgtcgagg ccggtggcaa gcgcacagcg cgacgttctg
     9721 atgcgggcag ccagggttcc agcggtagcg gccagacatt tatcatttct tgtcgcgcct
     9781 gagggcaatc agcggcaaca cgtttgccgt tttcatccag ccagatgggg aaacgaatgc
     9841 cattaatgcc ttcctgctcc ggcagtaata gagttggcgg ctggctcccg tccagcagcc
     9901 aggttgccag gcggcggcga cagttaccgt caccttccgg caaagactgt ccgcccggcc
     9961 agcagataac gccacgactt acagagtcag gacgtgggtc ttccggcaaa ttcacactgc
    10021 gcgacagtaa gatattatta acctgattca acagcggtac ggcactggca aagccaaact
    10081 gaccaacaac gggcgtgccg tccggtctgc cagtccagat cccaatgaca tagcgagcgt
    10141 taaccccaat cgcccaggca tcacgatagc catagctggt gcccgttttc catgccagtg
    10201 gggcgacgcg cggcaaggca ccatccggca agggttgcgc ttcatcagcc ataatccggc
    10261 gaatgatcca cgcagccccc gacgacatta aaggccgttc aagcagcgga tcgtcaggct
    10321 gtaagcgcaa tttacctgcc ttgccgtggc gagcaaacgc ggtatacgct gccgccatat
    10381 cttccagttt tgcgccagcg ccgccgagaa tgagcgaaag attcggtgca gcaccgttgg
    10441 gcaaatataa cggcaatcca acattgcgta actttgccgc aaaccgtttc ggtccatagg
    10501 cttccagcac ctgcacagca ggtaagttca gcgatcgcac cagcgcctca ctcatgctga
    10561 tcgggccatg aaaaccgctg tcaaagttgc caggtcggta atcaccggtg cgtctgggga
    10621 cgtcttgcaa cagcgatgcc gggtggatca agccttcatc cagcgccaga ccataaacaa
    10681 acggtttgag caccgatcct ggcgatcgga tcgcattgac catatcaaca tgaccaaagc
    10741 gtgaatcatc gttgagatca accgatccca cccagccgcg aacacgcata tcggtatgat
    10801 caaccacgat catcgccagt gagctgcgcg gtggcaatcg tcctttccag ttttgcgcca
    10861 gttcttccag acgtcgttga agaccggcat ccagcgtagt aacgattttg tcgcttttgc
    10921 ttttaccgag catcatgcgc gaaaacagcg gtgccagttg cggcatttgt cggggtgcca
    10981 gccagatggg ttcttccctt gactctttta cccgctcacg ggaccacaca ccttgcacgg
    11041 ccatccgttc gagcacttta ttacgcgcgg cttcggcacg ctccggccaa cgatccgggc
    11101 gaagacggct gggcacttgc ggcaacaccg ccagcattgc cgcctcggaa tagcttaaat
    11161 tcgcaggcga tttcccgagg taagcccaac ttgccgcacc aatcccctgc aacgtaccac
    11221 caaacggggc acggttaaga tacaaggtca gaatttcacg tttagacaga tgccattcca
    11281 gttgcaacgc gcgccagagc tggcgaattt tgccgccaaa cgttttgggg tgaggatcaa
    11341 gcaggcgcgc cacctgcatg gtgagcgtgc tgccgccgga aatgacccga cccgacgtga
    11401 gatcttgcca ggctgcacgc gccaccgaga acggattcac ccccggatgc ttccagaacc
    11461 agcgatcttc ataattgatc agcgcttcaa ggtaacgtgg agaaacatct tcgattgtta
    11521 ctggataacg ccagatgccg tcagcatcgg cgaaacgcca gagtggcgta ccatcctgcg
    11581 ccaccaccac gcgtgcagga ttcacttctt gcaatggcag aggccagagt ttatctgctg
    11641 cccatgctgc cagaatgatg ataaaaggcg cggctgccag cattatccag cagccgcgtt
    11701 tggttaacag gcgaggcatt tacggtctga ctatcaacag atcgtcagcc gcaccagtcg
    11761 cccgccattg aggaacatac attgattcca ccataggctg cggcacctga tacgttcccg
    11821 gcgtcaccgc ccgcgccaga tacaccagcg ttaccggttg atattcatca acggcaaccg
    11881 ccgccacaaa gcgatcgtca cggaactcta tgtgcttaat gctcgcctgc tgcatctggt
    11941 tcagcaagtt ctgcacttca ccaccacttt gctccaggct ggcgctgccg ttcgccagat
    12001 tctggttttc cagttccagc cccgcaggca acagatccac gactaacgca tccggtacac
    12061 tgttactggc tttaacctgc aaccacacca gcaccagatc gccgctacgt aacgagtcca
    12121 gcgatttgct cttaccgtca gtacccagaa tatgacgctc aatttgcagc acattgctcg
    12181 ccggtaaagg tgcggattgc ggataaccgc tggcatccac acgcagccat aacggctgat
    12241 cgccactgtt ggtcacctgc aaggtggcaa gttgatcgct attcagattg ctggtttgcg
    12301 ctttctcgcc tgtcaacggc tcagcagaga aagtggtttg cgcctgccat ttgcccggta
    12361 aatcctgaag agtacgggca gccaggaaca acgcgttact ttcctgcgtc gatagccagc
    12421 gttcaccgaa cgcctgctgc gaaagtgtgt ttaacaggga gtactgctca tcgggtagca
    12481 gtttgttctc ttccagcaat gagagcatca acgcgctgtc tcgcagtgga ctaccgtaat
    12541 cacccagcca tatccgctca tcactattac gcggcgtttt cagcgccagc gcaatcgctt
    12601 cttcaccacg cgtcgcatca cccatgattt tcagcgcaac gccaagttgc agcagcggta
    12661 aaccagaagc ggcatctgca cgatgctccc agatttcacg cagcgcaccc agtggtgctt
    12721 tttgctgacg ggccaacacc agcgcagcgt aagactgtac ggcgaattta ctggctttga
    12781 gattatcagc gtacgggatc gacatcatgc ccggatcttg taaatagcgc agcagacgct
    12841 cattaccccg gttaatggcg tctgtcggca cgctgtaacc ctgttcgcct gcgcggacca
    12901 ggaaatccat cacgtaagcc gtcagccagt actcttcgtc accgttttta tcccacaacg
    12961 caaagccgcc gttatcacgt tgcatttgca gcaaacggga aatgccgata tcgaccgatg
    13021 cacggcgttt ctcatcactg tcgcctttga tgcccaacgc ctgcagttgg gctgcattgg
    13081 tataaagtga cgggaacagg ccgctggcgg tttgctcgag acagccgtac ggatacgctt
    13141 ttaactcttt gatataacgt gcgatgttca gcggtggttt gccactcaac aacaattgcc
    13201 cttccagcgt aacaggcgag aagttttgca atccatcagc cgggagcgcc caggtttcac
    13261 caggctgtaa cgccgtaccg taattgaccg tttgtgccgg gaatgccgga cggacgccga
    13321 ttttccactg cttatgctga tcggcaacgg tttcccccgg taacgctaac ccgctaatgg
    13381 tggcctgaat gtctccatcg ccataacccg gcaatgcacg caccgggata aacagcgtag
    13441 tacgcacccc tggcgctaat tcaacaggtg cgggggaatt actcaccagt tcaagcaagc
    13501 cactggcggt cagcgcaaca ttaagttttt gcggtttatc ggtaagatta gtgatatcca
    13561 gcgtcaggcg cgaggtatcg ccactcgcca taaagcgcgg catgttcagt tcagcaatca
    13621 ccggtgcggc aacaatcact ttgctttcgt tgcttccgaa gtcatctgcc gtccaggctt
    13681 gcgccatgac gcgcaattca ccgttaaaat cgccaattgg cagtgtaacc gagccttcgc
    13741 cctgttcatt gagcgttacc ggcagcgcct gctgcgcgac aatattgacg tgattgaccg
    13801 gcggtttacc accacgtttc agctcatcac catcgccacc aaaacgcaga gctgccagac
    13861 gcccctgacc ttcaatgacc tgaccgtaaa tatcgtaaat gtctgcgcca tagcgtttct
    13921 gaccaaagaa cgcctgccac ggatctggcg tgacgtaatc agtaatattc agcacaccgc
    13981 tatcaaccgc agacaccagc acattcacct gttttggcat ctcaccattt ttattgctgg
    14041 ctttaatttt cacggttaat ggctgattgg ggcgcatttt ggtgggggtt tccagcgcca
    14101 gatcgaggcg acggttttca tcaccaagcg gcagatgcaa caccccaacc gcgcgttttg
    14161 gcgtcgcgga gcgagattta tcgccaggac gtaccaccag cgtacttaaa tacagatcat
    14221 gacgattcca ggttttatcg accggaatcg tcagatccag cccttgagcc ggaacatcaa
    14281 tctcttgcca ccacagcggc ccttcactgg actcgaccat cgcataacct ttacccgccg
    14341 ttggcgcggc aatatgcaac ttaatggtat cgccagggca atagctggct ttatccagtt
    14401 tcagcgtgac acggtcgggt cgcactgcac cacccccgtc gctgttatcc tgccagctat
    14461 acccagccca gaaacgcaca ctactgaccg cttcattcgg cgctttaact tccagacgat
    14521 aagcgcccca ctctaccggg aaactgacct taccggtttc gtccgctttc agatccagag
    14581 tttgttcatt ttcgatcaga tctttttgat caaactgtga ctgccagcct tcatattccg
    14641 accagttcca gtagtaatcg cggcgttcgc gaatcaagcg cacctgcaag cccgacacgg
    14701 cttttttcac gccttgcgca tcgctataaa cgatgtcaaa agcggcgtta ctaccttcat
    14761 caacaatggg ctgtttcacc gtgctgtcgg tgcgataatc gtaaaccgat ttcgaggcga
    14821 actgcggacg gatccctggc aatgcatcag caggccagat agcctgctca gcacggcgcg
    14881 tcaccgggcg accgcccgat tccagcaaac taccctggaa aataacctgt aatggggaat
    14941 gtgtttcctt ccactggctt tctgtagaaa cttcgccgcg ccctttatca tccagcgtca
    15001 actgaacttc atccagcgtg cgggaaagat tttcggcagc tatatcgccg aattcaaaac
    15061 caggtaaggc tgacacagct tcacgcagtg ggcgcaggaa aagttgccct tgcaaagtat
    15121 taccattcgc aggtgcacca tacaggtagt accccaccac ggagaatttc acttcatctt
    15181 tcggcgttag cggggttttc tcaccggtca gattcagcgc catacgctct ggcataaaat
    15241 cttcgacgtg gaaatcccac atccgatact ggttatcgcc cgtgttagcg cgaatatgcc
    15301 acataccggt tgccgcattg ctatcgagtg gccaggtaaa gtggtagagg ccattctccg
    15361 gctgactaac gacgctcctg agtatctgcc cgtcgggttt aatcacgtcc agcttgacag
    15421 gttgatcggg caacgcttta ccgtctgcat cacgcagcaa accattgagg atgaccgttt
    15481 caccagggcg ataaagatcg cgcgggccaa acatgaaaaa ctgtttgcta tagcctggcg
    15541 cgccagcaat gttaaattct gctaagtcca gcgccggaag ttttaaatcg agtagcgttg
    15601 tctgaccgtc tttacgcgcc agcagtaatg ccgcgttttt atcattttcc agctgcacat
    15661 gcccctgagc gtcactggtt gcctgagtca gagtctgccc tttctcattt aataaagaga
    15721 cttctattcc ttgctgggcc gcgccgtttt ccagactttg ggtaaagata tccagacgat
    15781 tgtgataacg gtgagctgaa acgccgatat cacttaacgt aaacagcgtc gcgggattac
    15841 tgtaatcgta acgtccagcc tgattcatca cagccagata cacgcccgcc tgctgaagcg
    15901 gtttgatatc gcccagcggc agcaataatt tttcacgggt gttacgcgca ggattgagat
    15961 caaaccgtcc ggtgtagacc agatccgcca tctgcagcag tttatctgac tgccagttcg
    16021 ccagcgaatt gcggtattcc cactggctaa tgaaagcggg cagtgattct ggtttcacac
    16081 ggaagaagtt aacatcgaca ttgttaacgt taagcgccat taccggcagc ccttcaacga
    16141 ccttaccagg aagcaacgaa ccacgactgg caaaaccgac gctgggttgg atatcgcggg
    16201 tagttatagt tttttcgtca tctttactaa aggttgcgtt attgagcgct ttgacctcct
    16261 tgccaatagt gacgatcaaa tcacgtttcg gttcgaggtg gcgtaaacgc agctctttaa
    16321 gattatctga cagctcccag gcaccatcca ctttgccgct ttttttatcg acgacatgaa
    16381 taacgcgtga gaaatcctga tccgggtcga gagggatgga gaacgtcagc accagcgtgg
    16441 ctgcgccatc gagttgaact tcagagagat ccagcaatgt cagcgcctta ccggcactct
    16501 gttgggccag tttttgtcgc tccggcgcgg aaagctttgc tgaactcacg ttttctgaag
    16561 aggccgcttt agtaacttca gaaggcgcat cttttttcac cgctgttggc gcgttatcgt
    16621 tgttgtcgca ccctgccagc gccagcatta gcatgcaggc ggctacgcgt aactttttca
    16681 tttttcatcc ctgccacaat ggcccgttag caacgtcgaa taattattat gcgtgagaat
    16741 ttacgttatg taattcagta tcacgcccat acaatccgaa ttttagtgag aattgtggct
    16801 cagacagcca aatcagcctc ttttgcttgt cgcttttcac taaacatccg acaatttagc
    16861 ctaaccccgg caaaaatgga gatgcctatg tccacgacat ggtttgtagg agccgactgg
    16921 ctcgccgaac atattgatga cccggaaatt cagattatcg atgcccgcat ggcgtcgcct
    16981 ggacaggaag atcgtaacgt tgctcaggag tatctgaatg gacatattcc cggcgcagtg
    17041 ttttttgata tcgaagcgct ttctgatcac acttctccgc ttccgcacat gctgccgcgc
    17101 ccggaaacgt tcgccgtggc gatgcgtgaa ttaggcgtta accaggataa gcacctgatt
    17161 gtctatgacg aaggtaatct tttctcagcc ccacgagcat ggtggatgct gcgcaccttt
    17221 ggtgtagaga aagtgtcgat tctgggtggt ggacttgcag gctggcagcg tgatgatctg
    17281 ctattagagg aaggcgcagt agagctgccg gaaggagagt ttaacgccgc gtttaatcct
    17341 gaagccgtgg tgaaagtaac cgatgtatta ttggcaagcc atgaaaatac ggcgcaaatt
    17401 attgatgccc gcccggctac acgttttaac gcagaagttg atgaacctcg cccaggttta
    17461 cgtcgcggac atattcccgg tgcattgaac gttccgtgga cggaactggt acgtgaaggc
    17521 gaactaaaaa cgaccgatga actggatgcg atattttttg gtcgcggtgt cagctacgac
    17581 aaaccaatta tcgtcagctg cggttctggt gtaacagcag ccgtggtttt gttggcactt
    17641 gcgacgctgg atgtgacaaa cgtgaaactg tacgacggcg catggagtga atggggcgca
    17701 cgggcagatt taccggttga gcccgtaaaa taagcttttt acaggcaata aaaaaccgcc
    17761 gaatttggcg gttttttatt gctggtctgg ttcgcggcct ttccagcagg ttgtattacc
    17821 gtagtaatgc aagcgcgtct cagcggacac aatactcgcc agtaactctc tttttgtcaa
    17881 gtaaaagaga gttattattg ttctgttagt gtattatcca ctgcggccct ttccgccgtc
    17941 tcgcaagcgg gcgctggctt aaggaaagga tgttccgcga ccgttagggc aagcgcgtct
    18001 cagcgcttgc tatcgcggca atatcgccag tggtgctgtc gtgatgcggt cttcgcatgg
    18061 accgcacaat gaagatacgg tgcttttgta tcgtacttat tgtttctggt gcgctgttaa
    18121 ccgaggtaaa taataaccgg agtctctccg gcaacaattt actggtggtt aacaaccttc
    18181 agaacagcaa gtaagcccga atgccgccct ttggggcggc atgttttaaa ttatccggtt
    18241 ctgtttaaag tcacgcaaaa aaccacccca gcgacgttca tagaatggcg caatatgttc
    18301 ggtaataaag tggctaatac ctttttcccc ttttttcacc tgacaaatat cgatgggttc
    18361 atcgccaggt aatgtatcgg tcgctacact tcccgccgcc tgaataattt cttcgatatc
    18421 accatcggct tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct ctttaattga
    18481 acaaataaaa gcacgcttca ccggcttaat ggttttaaat aaggtggtaa gtgaatcaat
    18541 catttgcgct ggcggctctg cgacctccga taatatcagc gattcaccgc cttccagtac
    18601 ttcctgactg ctaagcggat tcccttcttc accaatcagc aaactgattt cacgcggcat
    18661 aaattcttta ccggttggca gtttggcatt aaggaagagc gtttcgccaa gggtcatctc
    18721 aaacaatgtg cgaactggca ttacgacaaa tgcctgttcg ccttcaaccg cctgttgaag
    18781 tgcttctaac gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt cccagtgctg
    18841 taaatcaagt gcgctatctt caaccacagc ctcgccctgt gccgccgtac caggcaccca
    18901 gacggtggat tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt cggttgctgc
    18961 tttttccagc aggtcttcaa gttcgttttt tgtttcggac ataagaacca taattcattc
    19021 aacgttcggg gcgtaagtgc ctgatgcgct acgcttatca ggcctacaag ggaaccgcaa
    19081 tatattgaat ttgcaatact ttgtaggccg gataaggcat ttacgccgca tccggcatat
    19141 tagtttacgc cgttaacaga ttagcaatcg tgcgcacacc aagtcccgta gcacccgcag
    19201 accactgttc aaccggcgct ttacggtaag tcgccgagca gtcgatatgc agccaacctt
    19261 gctgatagtt ctcaacaaag tgcgacagga agcccgccgc cgtgcttgca cctgccggat
    19321 acgccgcgct tccggtattg ttcagttccg caaagttaga cggcagctgg ctgcggtgga
    19381 actccgccag cggcagacgc cagaacggtt cattctcctg cgcggcactc gccagcaagc
    19441 gccctgccag cgcatcatca aaactgaaca gcgcgtgata atcattaccc agcgcggttt
    19501 tcgccgcccc ggtgagggtc gccgcatcaa taatcagttc cggtttctgc gcactggcat
    19561 caatcaggcc atcggccagc accagacgcc cttcggcatc agtgttcatc acttcaactt
    19621 ttttaccgtt gcgataggta ataatatcgc ccagtttgaa cgcgttgccg ctgatcaggt
    19681 tatccgcaca acagaggaac agcttcacgc gcttgttcag tccgcgcgta atggcaaatg
    19741 ccagcgcccc ggtaaccgtt gccgcgccgc ccatgtccga cttcatcgag tccataaacg
    19801 cagtctgttt gatgctgtag ccgccggaat caaaagtgat gcctttacca accaggcacg
    19861 cgtacactgg cgcttcttta tcgccagttg ggttgtaatc cagcgccagc aataccggag
    19921 aacgttctga accgcgcccg accgtgtgca gccccatata accttgctca cgcagatctt
    19981 cgcctttggt gatccggtaa gtcacacgat cccctgcgac gttgctgatc agatcaacag
    20041 cacgctgtgc cagttgcgat ggccccagct cttctgccgg tgcgttgatg gtgtcacgca
    20101 cccagtcgat gatcatcagg cggttatcca gttcctgacg ctgggcatcg tccagatccg
    20161 gccactccac tttacgtatg cctttcgggg ctttgtaacc ttgccagaat gcccagcagc
    20221 gatccgcatc ccagccttca ccgcttaact gaacatgctt gatgcccaga ccgtcaatct
    20281 tgcgcgccgc acgctggatc aaccccagat cgtctgcccc gttcaaatgc agggtaatgc
    20341 catcattatt aatgctgtaa gtcgcttttt ctccccaacg tgcgtcggca ggttgggtcg
    20401 agagggtaat cttcatcgct tctgtcattt tagttatcct tattcgttta acagcaaaac
    20461 gggctacccc caggcagccc gttcatttac tcggcctcgt caagccagac caacaaaatc
    20521 gcttcgagga ttttctcgtt ggatgcctgc gggtcgtcgt cgaaatcttc cagatcgcaa
    20581 atccactgat gcatatcggt gaatcgaacc gttttcggat caagatcggg atacgcatcg
    20641 tacagtgctt cgccaatttc gcggctatcc gtccacttaa gtcccatact aacctctgtt
    20701 aatgctcacg cgcatggttg atagtgtaac gcgggatttc gaccactaaa tcttcgtcgg
    20761 taacgcgcgc ctggcagctt aaacggcttt ccggctccag tccccaggct ttgtccagca
    20821 tgtcgtcttc ctgctctgag ctttccggca gcgagtcaaa accttcacga acgatgcagt
    20881 ggcaggtggt gcaagcacag gatttttcac aggcgtgttc aatctcgata ccgttacgca
    20941 gcgcaacgtc gagaatggtt tcaccgctat tcgcttccag aacagcgcca tcagggcaga
    21001 gatcctgatg aggcaaaata acaatctttg gcatattaaa cctcgtccac ggaatggcct
    21061 ttcagagcac gacgaaccga ctggtccatg cggcgagcgg cgaaatcctg ggtttgtttg
    21121 tctacgtttt taatcgcttg ttcgatggcg tcaacatcat cgccctgcgc cacttcactc
    21181 aggtgagcgg cagcatcgtc gatagcctga cgttctgcgg cgcttaacag cgcggcatca
    21241 gcagccagcg caccgtgcag actttccagc acacgcgccg cttctacttt ttgttctgcc
    21301 agcattcggg cttttacgtc ctgctcggca tagctcattg agtctttgat catcgaggcg
    21361 atttcgctat cggtcaggcc ataagacggt ttgacctgaa tagacgcctc aacgccggtg
    21421 gatttctcca tcgccgtcac gctcaaaaga ccgtcggcat cgacctggaa cgtcacgcga
    21481 atatgcgcac cgccagccgg tagcgccgga atacctcgca gcgcaaaacg cgccagcgag
    21541 cggcagtcct gcaccagttc gcgctcacct tgcatcacat ggatagacat cgccgtctga
    21601 ccatctttaa aggtggtgaa atcctgggcg cgggccaccg gaatcgtggt attacgcggg
    21661 atcactttct ccaccaggcc gcccatcgtt tcgaggccca gcgacagcgg gatcacatcc
    21721 agcaacagca tttcgctgtc cggcttgtta cccaccagaa tatccgcctg aatcgccgcg
    21781 ccaatagcga cgactttatc cgggtcgata gaagtcagcg gtggacgacc gaaaaattcg
    21841 cctactcgtt cacgcaccag cggcacgcga gtagaaccgc ccaccatcac cacttccagc
    21901 acttcatcag cttctacgcc cgcatctttc agcgcgcgac gacaagccag taaggttcgt
    21961 tttaccagtg gcgcgatcag ttcattgaat tgttcacggc tgatttcgcc ctgccagccc
    22021 gcaacgttaa cggtcacgga gaccgcatcg ctcagcgcga ttttggccgc aatggcagca
    22081 tcgagcagtt cacgctgaac gcggttatcg ctacgatcag gaatgcccgc ctgctcgcga
    22141 atgtaatccg ccagcagatg gtcgaaatca tcgccgccga gcgcggaatc accgccggtc
    22201 gccagcactt caaacacgcc gcgacttaag cgcagaatgg aaatatcaaa cgtcccgccg
    22261 ccgaggtcat aaacagcgat cacgccttcc tggccggaat ccagcccgta ggcaatagcc
    22321 gcagcggtcg gttcattaag taagcgcaga acatgcagcc ccgccagacg cgccgcgtct
    22381 ttggtgccct gacgctgggc atcgtcaaag tacgctggaa cggtgataac gacaccatcc
    22441 agctcgcctg ccagggcttc agttgcccgc gccgccagtg ctttgaggat gtccgcagaa
    22501 acgcgcaccg ggttcagcag ccccgccgcc gtttcaatca tcggcaggcc attttcgctg
    22561 gcctggaatt gataaggcag atgcggatag cgttgctgga tatcagccag cgagcgcccc
    22621 atcaggcgtt taacagaact aatggtgttg gcggtgtcga gcgctgcgtt agtacgcgcg
    22681 tcataaccca ccgaatgccc ttgctgttga tagtgaacaa cagatggcag caggtgacgg
    22741 ccttcatggt cagctaacgt ttcggcctga ccgctgcgca ctgttgccac cagcgagttg
    22801 gttgtgccca ggtcaatacc ggccgccagg cgacgctgat gcggcgcagc actcaaacca
    22861 ggttcactaa tttgtaataa ggccatgttt agcttccaga aattaaaaat cgagcagttt
    22921 ttcttcgagt tgttcggcac tgctccgcag tttatcgaga aaacgcagct tacgcacggt
    22981 atccgccgcc gcgtcccacg tctcgttgtc taactgttca accatcaact gatggcgggt
    23041 atcaaacatc tttttcacac gtttgataaa gctttccagc cgcgcttcat ctttcgcctg
    23101 ttcgatctcg tccagttctt cgcgcagttc cagctgttcc atcaggaacg cggtgtcgcg
    23161 cacagtatgc tgctcgctgg cgagatcaaa accgtgcaaa gaaagcaaat attccgcgcg
    23221 cattaacgga tgacgcagcg tttgccaggc ctggttaatg gtcgcggatt gctgtacggc
    23281 ggcgagttgt tccgcctggc ttccgctggc gaatttatca ggatgatact gacgttgtag
    23341 atcctgaaaa cgcaggctca gcgcctgggt atcgagttga taacgggcag gcaagccaaa
    23401 gagggtgaag taatccataa cattctcagg gctgcgatcc gccgcgataa cggcgaatca
    23461 ggtaaaacaa accccacgcg caggcgacca cggtggggtt atcggtatgc gcatcaaacg
    23521 tggaagcttt cgccgcaacc acactcatct ttgacgttcg ggttggtgaa tttaaaccct
    23581 tcgttaaggc cttctttgac gaagtccagc tgcgtaccgt ccagaaattg caggcttttg
    23641 ccatcgacca cgactttcac gcctttgtct tcaaacacga tgtcttccgg cgtcggttcg
    23701 tcaacaaatt ccagtacata agccatacct gaacacccgg aggttctcac gcccagacgc
    23761 aggccaaacc ctttaccgcg gttagccaga aaggtattta ctcgcgctgc tgcactgtcg
    23821 ctcagtgtaa tcgacataac caaacctcaa ctcttatttt gcttcacgtt tgcttttata
    23881 gtccgcaatg gcggctttga tcgcgtcttc tgccagaata gaacagtgaa ttttcaccgg
    23941 cggcagttca agttcttcag caatatcggt gtttttgatc gcctgtgctt cgtcgagaga
    24001 cttccctttc acccattcgg tgaccaggga gctggaagcg atagcggaac cgcagccgta
    24061 agttttaaaa cgcgcgtctt caatgatacc ttcatcgttg actttaatct gcaacttcat
    24121 cacgtcgcca caggccggtg cacccaccat gccgctgccg acgttctcgt cgttgttgtc
    24181 aaaggaaccc acgttacgcg gattctcgta atggtcgata actttttcgc tgtaagccat
    24241 tataaattct cctgattccg ataccgatta atgatgagcc cattcgatgc tgttcagatc
    24301 cacgccctgc ttgtacattt cccacagcgg agaaaggtca cgcagacgac cgatggattt
    24361 acgaactaac tcgatggtgt agtcgatctc ttcttcagta gtaaaacgac ctaaagagaa
    24421 acggatagag ctatgtgcca gctcgtcgtt cagccccagc gcgcgcagca cgtaggacgg
    24481 ttcgaggctt gctgacgtac aggcggaacc tgaagaaacc gcgaggtctt tcagcgccat
    24541 aatcagcgac tcaccttcaa cgtagttgaa gctgacgttg agaatgttcg gcgcaccgtg
    24601 ttccaggtca ccgttcaggt aaacttcttc gatatctttg atgccgttcc acagacggtt
    24661 acgcaggccg cgcagacgtt ccatctcggt cgccatctct tcttttgcga tgcgataggc
    24721 ttcgcccata ccgacgatct ggtgaacagg cagagtgccg gaacgcatac cgcgctcgtg
    24781 accgccgcca tgcatttgtg cttcgatacg tacgcgcggt ttacgacgca catacagcgc
    24841 accaatacct ttcgggccat agattttgtg accggagaaa gacatcaggt caactttcaa
    24901 ctggctcaga tcgatcggca gtttgcccac gctctgggtt gcatcaacgt gatagataat
    24961 gccacgagca cggcacattt cgccgatagc cgcgatatcc tgcaccacgc cgatttcgtt
    25021 atttacgtgc atgatggaaa caaggatggt gtcgtcacgc atcgctgctt cgagttcttt
    25081 caggtcgata atgccattac gctgcggtgc caggtaagtg acttcaaaac cttcgcgctc
    25141 cagctgacgg caggtatcca gtaccgcttt atgttcggtt ttactggtga tgatgtgctt
    25201 gccttttttc tgataaaagt tggctgcacc tttgatcgcc aggttgtcag attcggttgc
    25261 accagaggta aagacgattt cacgcggatc agcgccgacc agatcggcaa tctgattacg
    25321 ggcgatatct accgcttctt cagcctgcca gccgaaacgg tgagaacggg aggccgggtt
    25381 accaaaggtt ccgtccatcg tcataaactg catcattttc tcggcaacac gcgggtccac
    25441 cggcgtggtt gcggagtagt cgagataaat cggtaatttc attgctctat aaactccgta
    25501 catcactcaa tgcaaggaac caggctaccg gttggatgta cgaccgtgtt tacggagtgt
    25561 ttagcactcc ggcctgattc tgaattcttt ttattaagcg cgtaacttaa cgtcgatcgc
    25621 gtcttgtgtg cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca catccagcac
    25681 ttcctggtta ttaaccagtt cgcctaaagt aatgttattg agaaaaccgg taagacggtc
    25741 gctcaaatca cgccacagcg cgtgggtcag gcatttatcg ccgccctggc agccaccttt
    25801 accctgacaa cgggtggcat ctacagattc gtcaacggcg ctaatcacct cgccaacggc
    25861 gatgctgctg gcatctttgc ctaacagata accaccgcct ggtccacgta cgctggaaac
    25921 cagaccattt ttacgcagac gggaaaacag ttgttccaga taagaaaggg aaattccctg
    25981 acgttcggaa atatcagcca acggtaccgg gcccgcttca gagttgagcg caacgtcaag
    26041 cattgcggtc acggcatagc gccctttaga tgtcagtctc atctcttact tcacctcaaa
    26101 ctcgcccctg cccggggttt tttattgtaa aagtgggggt attgcatagc agggtcaagt
    26161 ctgacattcc cgagtaaatt ggtcaactat ttacttgact gatttagtcg ggtatttaac
    26221 cttcagtgcc atttttttat cgtggcgttt gtacctgttg tcggatgcgg tgtgaacacc
    26281 ttatccgacc ttggattccg tgcacttaca ggcctgataa gacgcgttaa gcatcgcatc
    26341 aggcaaccac tgttattcgg ccttgttacc tttattctgc tgctcaatag aagccagaat
    26401 cccgcgcagg atattcaatt cctggctttc cgggcgtgca cgggtaaaca gacggcgcaa
    26461 tttattcatc acctgccccg gatggttttc acggataaaa ccggttgcca gcagcgtttg
    26521 ctccagatga ccataaaaac gctccagatc atcgaccagc ggatacggcg tctcttcatg
    26581 ctcgacctgc tcgccatttt cctgagttgc cagccaggcc atgcgcactt cataggcaat
    26641 aacctgaacc gccatcgcca ggttcagcga actgtattcc gggttagccg caatcgcgac
    26701 atgataatgg catttctgca actcttcatt ggtcaagccg acgcgctcgc gaccaaacac
    26761 cagcgccacc ggggtatttg ccgcttcagc gacgcttttc agaccgcatt cgcgcgggtc
    26821 gagcatcggc cacggcagcg tgcgggagcg agcgctggtg cccaccacca gactacaacc
    26881 ggctaacgct tcgtcgagcg tatcgacgat gtgagcatta ccaatcacat cgctggcccc
    26941 tgctgccagg gcaatcgcct gggagtctgg tttcactagt ggattaacca gccacagatt
    27001 ggttaatccc attgttttca tggcacgggc aacagaaccc atattgccgg tgtgtgacgt
    27061 ctccaccagc acaattcgaa tattttgcag cattgtcttt cttcatctaa agattattca
    27121 cgcatcttat cataaaacga agacagatgc cgatctcgct gctatactct gcgccgtttt
    27181 cccgttcttt aacatccagt gagagagacc gatgcatccg atgctgaaca tcgccgtgcg
    27241 cgcagcgcgc aaggcgggta atttaattgc caaaaactat gaaaccccgg acgctgtaga
    27301 agcgagccag aaaggcagta acgatttcgt gaccaacgta gataaagctg ccgaagcggt
    27361 gattatcgac acgattcgta aatcttaccc acagcacacc atcatcaccg aagaaagcgg
    27421 tgaacttgaa ggtactgatc aggatgttca atgggttatc gatccactgg atggcactac
    27481 caactttatc aaacgtctgc cgcacttcgc ggtatctatc gccgttcgta tcaaaggccg
    27541 caccgaagtt gctgtggtat acgatcctat gcgtaacgaa ctgttcaccg ccactcgcgg
    27601 tcagggcgca cagctgaacg gctaccgtct gcgcggcagc accgctcgcg atctcgacgg
    27661 tactattctg gcgaccggct tcccgttcaa agcaaaacag tacgccacta cctacatcaa
    27721 catcgtcggc aaactgttca acgaatgtgc agacttccgt cgtaccggtt ctgcggcgct
    27781 ggatctggct tacgtcgctg cgggtcgtgt tgacggtttc tttgaaatcg gtctgcgccc
    27841 gtgggatttc gcggcaggcg agctgctggt tcgtgaagca ggcggcatcg tcagcgactt
    27901 caccggtggt cataactata tgctgaccgg taacatcgtt gctggtaacc cgcgcgttgt
    27961 taaagccatg ctggcgaaca tgcgtgacga gttaagcgac gctctgaagc gttaatgact
    28021 caggcgggtg atatcactca cccgccctcg cctttcaggc gctattccga aatacttcct
    28081 caccgcttta ctttctttcg tcactctccc accatctttc cccgattaat ggatgataaa
    28141 gaagtcgcaa ggaaaggtta tggcgctgcc agtgaacaaa cgcgttctca aaattctgtt
    28201 tattctcttt gttgttgcct tctgcgttta tttagtgccg cgcgtcgcca tcaacttctt
    28261 ctattatcct gacgataaaa tatacggtcc cgacccctgg tcggcggaat ccgtcgaatt
    28321 tacggctaag gacggtactc gtctgcaagg ctggtttatt ccttcttcga cgggccctgc
    28381 tgacaacgcc atcgcaacca tcattcatgc tcacggcaat gccggaaata tgtccgccca
    28441 ctggccgctg gtcagttggt tacccgagcg taacttcaac gtttttatgt ttgattatcg
    28501 cgggtttggt aaatcaaaag gcacgccgtc ccaggccgga ttgctggacg atacgcaaag
    28561 tgccatcaat gtggtgcgcc atcgcagcga tgtaaaccca caacggctgg tgctgttcgg
    28621 gcagagtatt ggcggggcga atattctgga tgttattggt caggatgatc gtgaaggcat
    28681 acgtgcggtg atcctcgact ccacctttgc ctcttatgcg accatcgcca accagatgat
    28741 ccccggcagt ggctacttac ttgatgagag ttacagcggc gaaaattaca tcgccagcgt
    28801 cagcccgatc ccgcttttac tcattcacgg taaagctgat cacgttatcc cgtggcagca
    28861 cagtgaaaag ttgtatagcc tggcaaaaga accaaaacgg ttgatcctaa tcccggatgg
    28921 cgaacacatt gatgcttttt ccgatcgtca cggcgatgtt tatcgcgaac agatggtgga
    28981 ttttatcctt agcgcattga atccgcagaa ctaacctatg atcgctagca cgataatcat
    29041 tcacaaaacc accttaagac atgctaatcc actggtcaga acagtttaag atgagaaaaa
    29101 ttctgtgatg cttgccaaca tttctgatga ttagcattcc cttcgccatt tccctgagca
    29161 aactttagct attcttatca attatgctta tgggagatct acagatgatg cctacgcttg
    29221 ctccaccatc tgtcctttcg gctccccagc gccgctgcca gatcttgctg acactctttc
    29281 agccggggtt gaccgccacc acggcaactt tcagcgagct taatggtgtg gatgatgata
    29341 ttgccagtct tgatatcagc gaaacaggac gggagatcct gcgctatcat caactcacac
    29401 tgactactgg ttatgacggc agctaccggg ttgaaggtac agtgcttaac caacgtttgt
    29461 gtttatttca ctggctatga cgtggtttcc gtctgtgtcc gtcatttatt accagccgat
    29521 tcacccccgc cctgaagagt gaactgaagc ggcgcggaat tgcgcgtaac ttttacgacg
    29581 ataccaatct acaagcgtta gtgaatctct gctctcgacg gctgcaaaaa cgctttgaaa
    29641 cgcgcgatat tcatttcctg tgtctgtatt tgcaatattg tttgttgcaa catcacgctg
    29701 gaattacgcc tcagtttaat ccgctccaac gtcgctgggc agaatcctgc cttgaatttc
    29761 agatagcgca ggaaattgga cgccactggc agcgtcgggc gctccagcct gtaccgcctg
    29821 atgagccact atttatggca ctactttttt ccatgttgcg ggttcccgat ccactgcggg
    29881 atgcgcatca gcgggacaga caactgcgtc agtctatcaa acgtctggta aaccattttc
    29941 gtgagctggg aaatgttcgt ttttatgatg aacaggggtt atgcgatcag ctttataccc
    30001 acctctccca ggcgttaaat cgcagtttgt ttgccatcgg tattgataac accctgccgg
    30061 aagagttcgc ccgcctgtat ccacgcctgg tgcgcaccac ccgcgcggcg ctggccggat
    30121 ttgaaagtga atacggcgtc catctttctg atgaggaaag cggtctggtc gcggtgattt
    30181 tgggtgcctg gctaatgcag gaaaacgacc tgcatgagaa acagattatt ctactgaccg
    30241 ggaatgatag cgagcgagaa gcgcagattg aacagcagct acgcgaacta acgttactgc
    30301 cgctcaacat taaacatatg tcggtaaagg catttttgca gacaggcgct ccgcgcggcg
    30361 cgacactgat tattgcgcct tataccatgc cgttaccgct cttttcacca ccgctgatct
    30421 atacggacct gacgttgaca acacatcaac aggagcagat ccgcaaaatg ctcgaatcag
    30481 catgagggaa caactttcgg gcgcaaaaac attgccggaa gcgccaccag cgccatcacc
    30541 cagaacacgc cgtggccgag atattgatac aggaaaccgg cgaaaacggt catgatggcg
    30601 atactgccgc ccatcgcgac ggcagagtaa accgcctgta aacggatgac ttcgctaccc
    30661 tggcgagcag caatatagcg catggcagcc aggtggcaga ccgtgaaggt accgcaatgc
    30721 agaatttgca ccactatcaa ccacggcaac gccgtagttg ctcccataat gccccagcgc
    30781 actacgccgc aaatcgccga gatcaacagc atatcgcgtg cactacaacg gcggaaaagt
    30841 ttattactca gcgcaaagat aatgacttcc gccaccacgc ccagcgacca caaatacccc
    30901 accgccgagg ccgagtagcc agctgcctgc cagtaaatgg cgctaaaacc gtaataggcc
    30961 gcatgtgccc cctgcaataa acaaacgcag gccagaaagc gccagttctg gcgaaccaac
    31021 gccaaccacg cagaccagcc ggtgctctcc tgctggcggc ttgccccttg tggctgaatc
    31081 gtcggacgga tgagaaagcc gagcagcatg gatgccacgc ccaacgtcaa cagcgcgagg
    31141 atcacccgat aatcaaacat actgaccagt ttgcccgtca gcgccgagcc aatgacaaac
    31201 gccaccgagc cccacagtct cactttgccg taatcaagcg ggaactgctt ttgccacgta
    31261 ttcgccagtg catcggtcaa cggtaccagc ggtgagaaaa agaggttaaa gccaatcatc
    31321 accagcatca gccacgctac gtgcgccccc gcccagaagg cgacagcaaa gagaagtgtc
    31381 agcagtgcca gcacgcgcaa ggcggaaatc aggcgggaag gatcgctgac gcggggcgcg
    31441 atgagcaaac tcccgaggaa acgggcaacc agacccaccc ccaataacag gccgatggtt
    31501 tctggcgtta aaccaatccc tttaagccag acgctccaga aaggtagaaa aatgccgtaa
    31561 ctaaaaaagt atgtgaaata gccgagcgcc aaccagcgcg tggattgcaa aaccatgagt
    31621 ccctcccgtc aggaggcgtt agtctgaggt taatcgaatg ttggtgcaag ttgaaaaata
    31681 agaatattcg agcctgcttc ctgacggtta tcctgcatat caggaaatga caaaaaagag
    31741 cggcccacaa cacaacatgt aaaaaagaac gatgaaacag gtgaaaatcg caatttattg
    31801 aactgacaaa ctcatgtgag acggataagg cgtgcatgtc gcatccggca ggaaacaata
    31861 agcacattgt cagccatctg cgttatgccg ttataccaga aacgaatatc tcaacgcctg
    31921 ggcgtggcga ggtaccagag tgtgtattac gtttaaatca catcatcttg caaagggatt
    31981 ggttatgaac tcactacgtt attttgattt tggcactgcc cgccccgttt tattattaat
    32041 tgcccgtatc gccgtggtct taattttcat tatttttggt tttcccaaaa tgatgggctt
    32101 tgacggtacg gtccaatata tggcctcgct gggcgcaccg atgccgatgc tggcggcagt
    32161 tattgcggta gttatggaag tacccgccgc gatattaatc atacttggct ttttcacccg
    32221 tccgctggcg gtgctgttta ttttctacac gctgggaact gcggtgattg gtcatcatta
    32281 ctgggatatg actggcgatg cggttgggcc aaatatgatt aatttctgga agaatgtcag
    32341 tatcgctggc gcattcctgc tgttggcaat taccgggccg ggggcgattt ctctcgatcg
    32401 ccgttaggaa agatgccgga tgcggcgtaa acgccttatc cggcctaccg ctccaacacc
    32461 gattgtaggc ctgataagac gcagcaagcg tcgcatcagg catcgtgcac cgagtgctac
    32521 aaaatcgaaa ttaccccgta ggcctgtgca tccggcattt tataaattac agcaacgcaa
    32581 cccgcaacga catctctgaa attgttgatt cccctggtgc aagcgcaatg aggtcaccac
    32641 cttccggacg atgatggtca tccggcgcat ggctcatcgg ttccagacag aagaaatcaa
    32701 acgcatatcc tttatcaaac gccgggtcgg aaacaaagat gaaataacac ggtgcaggcg
    32761 gcgtcgtttc catgatgatg gcataaccct cctgcggctg ctcgatgcgg gcctgaccat
    32821 tccatccggc aaaaccattg tttacccact ggcgcggtaa cggcgcgagc tggttaaaat
    32881 ccagttcctg cggtagctgc tcgcaaaact cacccgccag ccactgctcc tgctcccgcc
    32941 agtaaccgct cgcctgcgcc tgaatccgcg tttgtggtga caacggaaaa taaggatgcc
    33001 agccggtacc aaatggcagc gtctctgccc ctttattggt gacagcgagc gtcaccgtca
    33061 gcgtatccgc cgttaaatga aacgcctgac ttacccgata gtgataaaca ccgctgcgat
    33121 gttcatacac cagacacaag ctatcttcgc tgcgcgagac acattgccac tgccccagcc
    33181 aggcatcacc gtgtagatag tgcgcatccc actcaacatt cggttgtagt tgatattcac
    33241 gtccctgcca gacaaaacga ttaccgctca cccgattagc aaacggaacc agcggaaaac
    33301 aggaagcgtc ggtagcaacg ccgctttttt tccccggacg cagcagcggg gcggcgtcac
    33361 gccagaagcc ttcaattaca ccgccctgat cggaaacctc aagctttagc gccccctgag
    33421 atagggtata gatggtcatc ctctactcct taatccggga agttgatcac gactttcccg
    33481 catttgccgc tcgccattag cgcgtaagca tctcccgcct gctccagtga gaagcgatgg
    33541 gtaatagcgt tacgcggcca cagtttccag tcagtcaaat cgtgggcgca tttttccata
    33601 tggaacaagc tggtcaccca ggagccaata attctgcgtt ggtgatgcat caggtcagcg
    33661 ctgacctcaa attccacttt tccggtttcg ccaatgtaaa ccactcgccc ccagtcggcg
    33721 gtagattgca atgccagcaa gcgccccgca gcattaccgg aacaatcaag cgcaacatcc
    33781 gcaccgccat gagtgagttc agcgatgatc tgcggcagac cttcggtggt cgctaaatag
    33841 ccgtgatcca tcacccctag ctgtttcgcc atcgccagac gttccggcaa catatcaacg
    33901 ccgatgatcc tttttgcacc acgacctttc gccagcatca tcgccatcat gccgaccggc
    33961 cccagaccga ccaccagtac gttatcactg ccggaaactt cgccgcgcaa aatcccttca
    34021 tacgctgtac caacgccgca actaataaac gcaccatctt cgtaactcag tgcatccggc
    34081 aggaggatca gatctttttc ttccgccagc aggtattcag catggccgcc gtcacgctgc
    34141 cagccgtaag ccgcttttcc ttcgccagta caagagatag gaaaaccacg acggcagttc
    34201 gggcaaaaac cacagccaga aatatgatac accagcacac ggtcgccctc tttaaaatgg
    34261 cggcagcctt gccccatcgc cacaatctgc ccgcacggtt catgaccgtt gataaatccc
    34321 tggtataacg gtttatcggg tgccgccgcc gtagcacggt gctggtggta gatatagtgg
    34381 acatcgcttc cgcaaatccc ggaggatttc attttgatca gtacctggtt aatccccggc
    34441 gtcggcaccg caacttcccg cagatcgacg gtcgaatttc ctggtaaata agctgccagc
    34501 atcgttttca taaatccctc attaacaata cgattaattt tcatccctgc cgcacccgcg
    34561 ccaggggcgt taaggtttag cgtttcgctt tactgctacg ctgggtcagc aagatattcg
    34621 ccagcaccgc caccacgatg atgacgccgc gcaccacctg ctggaaaaag gagttaatcc
    34681 cgagcagcac cagaccgtta ccgattagcg taatcaccag tacgccaagc agcgtaccga
    34741 acaacgagcc gcgaccgccg gaaagcgccg taccgccgac cacgaccgcg gcgatgacgt
    34801 caaactccag accgtttgcg gcacctgcgt taccggaacc gaggcgggcc gccaacaaaa
    34861 tgccggtcac cgccgctaat aatcccgaaa gggtaaaaat aagaatgcgt acccgacgaa
    34921 cgttaatacc gcacaactgc gctgcagtgg cattaccgcc aacagcaaaa accgagcgcc
    34981 cgaaggcagt tttgcggcta atgaacacaa acagcgcaaa taacaccatc atgatcagcg
    35041 cggataccgg cacaccgaga aattgtccgc ccagccagtc cagcacttcg ttttcgttaa
    35101 ttggcactgg caaggcgttc gtcataaaca gccccattcc gcgcagggcg ctccataaac
    35161 ccagcgtggc aacaaaactt ggcacgttaa acacgccgcg cagcaccccg gcgagcgttc
    35221 ccatcagcgc acctaacagc aacaccagca gacaagcaat cgccagcgga acgtcaaatt
    35281 gcagcaaaaa cgccaggcac actgaaacaa aagccaccat cggcccaacg ctgacatcaa
    35341 tttcaccgga gataataatc agcgtcatcg cccaggcggc gatcccgatg gtggcggcat
    35401 cgcgcagcac gttcatttgg ttattcaatg agataaagcc aggcgcattc agggagaaga
    35461 ccagataaag aatggcaatc accaccagca aaccgatctc attaatatgg cgactgacaa
    35521 attgtttgag cgagacgctc ttgccctgcg gcaatggtaa tgacgaagca gacatcttca
    35581 ggttcctcgg taaaattcat cagtgcacag acagaatggc ggacatcagc tcatccacat
    35641 tgaccggaga gtgaaactcc tgcgagaacg taccgtgctg taacaacaga atgcggtcgc
    35701 acaccaacgg taactcctcc acttcactgg agataaacac cacgcttttt ccttcggcag
    35761 ccagctcgcg gacaatacgg taaatctgct gtttggcttc gatatcgacg ccgcgcgttg
    35821 gctcgtcgag caacaaaatc tggctggcgg catagaccca acgaccgatc accacttttt
    35881 gctgattgcc gccagaaagc gtgccgatgg gcgtttcgct actggcggcc ttgaccgtca
    35941 tccgctgcat cacctcttcg gtcaggcggc ggatggtgga ccattgcagc acaccgttgg
    36001 cgctgatttt ttgccgattg gtcagcactg tattttcgtc aacgcccaac cagggaatga
    36061 tccccgcttc tttgcggttt tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt
    36121 aatcggggcg cgtgattttc tcgccgttga taacaatttc gccctgttca tacgtctcca
    36181 gaccaacaat cgccttcagc aattcactgc gccctgcccc cagcagacca gcaatgccga
    36241 gcacttcgcc acgacgtagc gtaaaactga tatcctccag cttgggctta tggcgtaacg
    36301 cacggacttc cagcacggcc tgatccataa tttcctgagg ggcaaccggc gcaatatcaa
    36361 cgtgatcgcg cccgagcatt agcgacacaa tatgatgcgt agaggtgttt tcgagcatca
    36421 catcgcccgc cacctgacca tcgcgcataa cggtggcaca ggaggcaatg cggcgaattt
    36481 cttccatccg gtggctgaca taaatcaccg ccacgcccag tgccgacatc tttttcaccg
    36541 cgctgatcac cagttcaact tccgcactgg caagcgagct ggtgggttca tcaagaatga
    36601 ccacgcgcgg ctcgcccttc atcacccgcg caatttccac cagttgcttt tgcgccgggc
    36661 ttagtgttga aacaagttgt tcaggactaa cgtcaacgcc caacgcctgc aagcaacgtt
    36721 gggcgtcctg cgccatttgc aggtaatcaa tcatgccgtt gcggcggggc cactgaccga
    36781 ggcagaggtt ttccgccact gtcagccctt ccaccagact taactcctga taaaccgcac
    36841 gaacccccag ttcagcggca cggcgagtca gcgtagcttc gtcaccttcc agtcgcgtct
    36901 cgccaatcca gatatcaccg ctatccggac gttcgctacc ggtcagcatt cgaatgaggg
    36961 tcgattttcc cgcaccgttt ttgcctaaca gcgcgcgaac ttcgccttta ttgagcgtga
    37021 agttaacgtt atccagcgca acgacgccgg ggtaacgctt atttcctgcc accacttttg
    37081 ctaccgggac tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag cgattagggc
    37141 agaccatcaa cgtgcgttgc cagccactgt ttaccgtctt ccgttttggt atagagatcg
    37201 attggcacct gaatcacttt ttcaccgtcg gcttgtttat tgataacctt caatgtttgc
    37261 gtaaaaacag cattgcccat tttcttaccg gaaatatcca ctaccgcttt cagcacctgg
    37321 ttattttcca gttcctgagc aatttcagtg gtcatatcag aaccgaaaac agcaattttt
    37381 ccggcctgat tttgattacg taccgctttt accgcgccga gtgtcgcacc gcccgattcc
    37441 cccataatgg cgttgagatc cggcgtggag ataatcagtt tttcaccgac agaaatcgct
    37501 ttatctaaaa cagtcccttc ctgattagcg acaatttgcg cgccgggaac gtgggttttt
    37561 aatacttctt caaacccttt acgtcgttgc acacaaactt caaaggcttc gcaattgatg
    37621 acggcaattt tcggctggtc aattttgttg gcaataaaat aatcggcggc tgcgttaccc
    37681 agttttttac caaattccag cgggtcacca accagatatg ccgagacata tttatcgaca
    37741 cccttttgat taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga
    37801 cgaacggtac ggctactgcc attttcagac actgccgata aaataatggc atcgacatta
    37861 cgcgccacga gggtatcaac aaaggtactt tctttcgaaa tatcgccctg agcgttagtt
    37921 tcaattaatt gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact
    37981 ccggcgtaat atccctgggt atcaaggtat attgcgccaa tagtcatttc tttatcagaa
    38041 gccctggcaa atagtgcgct acccaatagc gtagccatta ataataaatt acgggttctt
    38101 gtcatttttt taggcatagt gcctcctgta gggtttttat ttacaacggc ttattttaat
    38161 tatttgagcg tgagcggcag cgcgtgccgc tcaaatatta cagttaatag ggtttagtga
    38221 acgtagctaa aaataaaagg catcacggtg gtgaataatg ccgctttcgg ccaggctgga
    38281 ttaatcgcca tcaattcagc gcgagcagat tcatagtgcg caacctcccc taatgccgcg
    38341 gccgccagca tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgctatac
    38401 agcgacaaca agtcaggctg cgagacggca aagaaatccg cttcgatact ggttttcgcc
    38461 atctctttcg cccactgttt catttcacta aacagttgct gtgcggtgtg ttgttcgccc
    38521 agcagtcgca gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa
    38581 ctgtggatgt taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca
    38641 gtttcatcgc cctgcgcgtt ggcgcatact gcctgccaga accagatgtc gttgtcagtt
    38701 tgccccggta aacggccttc gcttaaattc tccggataat gcagcgcggc atgaagcagt
    38761 tcgctggcct gctgcggctc tctggcatca agatgctgcc aggcgcgtaa taactggttg
    38821 aggataaact ggctggtgac cttcccttcc ccaccttccc acgggtggaa tttgcgcgtg
    38881 gcgagaatgt ccgccgcttt gtctgcctga ccagtgagat gccacaaatt gagcagttct
    38941 gcggtcatgt cgtcgcgttt cagcgcaatt tccagattat ttcccagacg cgccagtcgt
    39001 ttctccggtg tgacaccgct taacttatcc agcaaatccc gttcgaaaag cagacgtgca
    39061 tcctgcggcg caagctgata agcattatca agataacgcg cggccagctc ataatcgtgc
    39121 tgcttattcc acgcatggat cgccaacccg cgccagccgt cggcaaactc cggcgacatc
    39181 tctacgcaac gctgccataa ggcaatggct ttaccgtagc tacgcttgtt gtagtagaag
    39241 caggccagta aatgccgggc aaaccagcac tcttcgatac tctccagcgc cgccacttct
    39301 tccagcgtat tcgggaaacg gacaaactgc gggaaggcat caatagcttt tacgaccagt
    39361 tctccgcgtt cagcttttgg tagcaaactg gcttgcaggt aaagcggtag cgtgcgctga
    39421 cagtccagtg cgttcagcat atctgccgcc agggcgggca tgccccagtt aatcagttgc
    39481 acggcggtca tcagggcgtt aacgtcgcgt ccctgacaaa gacctcgcca ctgtacgagg
    39541 gcagattcgc tacgaccatc gaaccagttc agccaccaca gagtagcgtt cagcggataa
    39601 tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct gacgaccact taacaccagt
    39661 agcaggttat gcaggcaaag cacttcctga ttggttgggc tggcgcgaag actttgttgg
    39721 caaaaatcca gaccagcgtc aaagttacca ttacgcgccg ccagccgtgc cagaccataa
    39781 tagccgcctg ctttactgtt gccgctccag accgcacgcc agaaatcctc ttcggcttgt
    39841 tgatattgcc cctgacgttc gtaagcactg gcgcgaatca aactcgcctg tccgcactgc
    39901 ggatttttgt tcagcgcatg tgcgcgtttc agcgcctgag tggcatacgc caccgcttgc
    39961 gggaaatcgg cacggttata ttccagcatc gccagcgcca ggttacagcg ataatccagc
    40021 ggatccagcg ccacgccgcg caggtagtaa tcgaacggtg aacggctcgc gtgatgatat
    40081 tgctccagat gttgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgcc
    40141 agtggcgctt tggcgacgtc cggcaacggc aacgcttgcg gctgattttc atgataactc
    40201 agtacaatat tgccgtcggc atcggagagc tcaatggtca gcctttcagg attgataccg
    40261 tgcaacacgc cctggatggc ggtcgctggt gtcagggcca cggcatcatc gagcaacgcg
    40321 ttgcatttgc cgatttcgcg gatcgccagg cggtatccgt tcaacggaga gatggcatac
    40381 agcccccact caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca
    40441 ttttgcacca tgcccagaga atgataaggc aggaaatact gctcgaaacg cttctcctcg
    40501 taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa aaaaaccggt catcagttcg
    40561 atatacgggc cgttattgtc agtcaggctc ttatcccacg cctggccaaa ttcactgtgt
    40621 ccccagctcc actgtttttt acctggcgca atatggtggt tggcaacgtg tagcaaacca
    40681 ccatcttcat cgtgacacca cgcgccaaca aaatcgtact gtgatttttc agccatatat
    40741 gaggttggaa cgggcacatt tttatagcga gaaatgtcca ctccggcgga gtagtccact
    40801 ttgtagtaag tgccggtggc gatggggaaa gcggagacgg cccgtttacc gtgatcaaac
    40861 accgcagtca catccggtgg gaaaacgctc tgatgccctt cccccccttt cactgccggg
    40921 ttggcccacc acaagaaatg acgcggcgtg gcgttgccgt tatagacgcg actggcgatt
    40981 tccagcgccg cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accgtgcatc
    41041 ggctccgttt cgccgaccca caccgtctgt gcgccgtcgt catgggcttc gagggtgaaa
    41101 tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccgccg
    41161 gagatccacg gccccagcag tcccaccagc gccggtttaa tgacttcatt gtgataaaca
    41221 aaatcgcgct gtttcacttt atcccacgcg cgatgcaccc gaccgcccag ttccggcagg
    41281 atcatcactt tgatgtagtc gttttccagc cacaccgcct gccaggattt cagggttttc
    41341 tgctcgctca gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa
    41401 acgcgatttt ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc
    41461 tcaacgcgct cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat
    41521 actgaattaa aagatttgtg gcagtgtatt gaacaatctg gcactgtttt cgcggaataa
    41581 tcacgcaatt aactaaacag ggtttagtga agatgagagc ctgcattaac aatcaacaga
    41641 ttcgccacca taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata
    41701 aatcaacgct ggcccggctg gcgcaaattt cgattccggc ggtcagtaat attttgcagg
    41761 aactggaaag cgaaaaacgg gtggtgaata tcgacgatga aagtcagacg cgcggacata
    41821 gtagcggtac atggctgatt gcgccggaag gtgactggac gctgtgtctg aacgtgacgc
    41881 ccaccagcat tgagtgtcag gtcgctaatg cctgtttaag tccgaaaggc gaatttgaac
    41941 gctttcagat tgacgctcca acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc
    42001 atcgccaccg taaattgtgg ccggaccgca ccatcaatct ggctctggca atccacggtc
    42061 aggtggatcc ggtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg gcaacaccga
    42121 ttgaggtgaa gtacctgctg gaagagaagc tgggcattcg ggtaatggtc gataatgact
    42181 gcgtgatgct ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg
    42241 taatcaacgt tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg
    42301 gcagtttgta tggcagtgga cagattggtc acaccatcgt taatccggat ggcgtcgtct
    42361 gcgactgcgg gcgttatggc tgcctggaaa ccgtcgcctc gttaagcgca ttaaaaaaac
    42421 aggcgcgggt atggctaaaa tcacaaccgg ttaatactca gcttgatcct gaaaaactga
    42481 ctaccgcgca gttaatcgct gcctggcaga gtggagaacc gtggatcacc agttgggttg
    42541 atcgctctgc caacgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta
    42601 atcagatttg gttgtacggt cgcagttgtg cctttggtga gaactggctt aataccatta
    42661 ttcgccagac gggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc
    42721 aaattggctt tgggcaatta agccgcgcac agcaggtgct gggaattggc tatttgtatg
    42781 ttgaggcgca gttacggcag atttgatggc gcaataacgt agaaaggctt cccgaaggaa
    42841 gccttttttc aacagattaa ccgtttcgct tatgcgtaaa ccggataacg tgcgcagatg
    42901 tcgagaactt tacctttgat gcgctcgata acagcttcat cattgatgct gtccagcacg
    42961 tcacacatcc agccagccag ttctttcgct tcggcttctt taaagccacg acgggtaatc
    43021 gccggagtac ctacacgaat accggaggtc acaaacgggc tcttcggatc gttcggtacg
    43081 ctgtttttgt tgacggtgat gttagcacgg cccaaggcgg cgtcagcttc tttaccggtc
    43141 aggtttttat caaccagatc aaccaggaac aggtggttat cagtgccgcc ggaaaccact
    43201 ttgtagccgc gttccaggaa cacttctacc atcgctttag cgtttttcgc aacctgctgt
    43261 tggtaagttt tgaactcagg ctccatcgct tctttcagag caaccgcttt accagcgatt
    43321 acgtgcatca acgggccgcc ctgaccacca gggaaaacgg cagagttcag ttttttgtac
    43381 agctcttcgc taccaccttt cgccaggatc aggccgccgc gcggacccgc cagggttttg
    43441 tgagtggtgg tagtaacaac gtgagcatga ggaaccgggt tagggtagac accagcggca
    43501 accaggcccg caacgtgcgc catatcaacg aacaggtaag caccgatgct gtcagcgatt
    43561 tcacgcattt tcgcccagtc aaccacgccg gaatatgcag agaagccacc gataatcatt
    43621 ttcggtttgt gttctttggc ttgtttttcc agatcggcgt agtcgatatg accggtagca
    43681 tcgataccgt aaggaacgat gttgtacagt ttaccggaga agttaaccgg agaaccgtga
    43741 gtcaggtgac cgccatgcgc cagattcata cccagaacgg tatcgcctgg ttccagcaac
    43801 gcggtgtaca ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt agcgtagtca
    43861 gcgccgaaca gttctttcgc acgatcgatc gccagttgtt caacgatatc aacatactcg
    43921 caaccgccgt agtagcgttt gcccggataa ccttcagcat atttgttggt cagctgagaa
    43981 ccctgcgcct gcattacgcg cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc
    44041 tcttcctgac gtactttttc ctgctccata gcctgccaca gttcggcatc ataatcggca
    44101 atgttcattt cacgctttaa cattcgcatc tcctgactca gctaacaata aaatttttgg
    44161 cctttatagg cggtcctgtt ggacaacggc gaacagtata accgaatcat tgtgcgataa
    44221 caggtcttga caaaggaatt tacgcaaacg attaccttca ggctacgcaa ggctttggag
    44281 aataaagagc ttgtaaccgg aaacggattt cttttcaggt ttgtgatgca aatttttcac
    44341 ttcatcacat tctttctgaa aaacaccaaa gaaccattta cattgcaggg ctatttttta
    44401 taagatgcat ttgagataca tcaattaaga tgcaaaaaaa aggaagacca tatgcttgac
    44461 gctcaaacca tcgctacagt aaaagccacc atccctttac tggtggaaac gggaccaaag
    44521 ttaaccgccc atttctacga ccgtatgttt actcataacc cagaactcaa agaaattttt
    44581 aacatgagta accagcgtaa tggcgatcaa cgtgaagccc tgtttaacgc tattgccgcc
    44641 tacgccagca atattgaaaa cctgcctgcg ctgctgccag cggtagaaaa aatcgcgcag
    44701 aagcacacca gtttccagat caaaccggaa cagtacaaca tcgtcggtga acacctgttg
    44761 gcaacgctgg acgaaatgtt cagcccgggg caggaagtgc tggacgcgtg gggtaaagcc
    44821 tatggcgtcc tggctaatgt atttatcaat cgcgaggcgg aaatctataa cgaaaacgcc
    44881 agcaaagccg gtggttggga aggtactcgc gatttccgca ttgtggctaa aacaccacgc
    44941 agcgcactta tcaccagctt cgaactggag ccggtcgacg gtggcgcagt ggcagaatac
    45001 cgtccggggc aatatctcgg cgtctggctg aagccggaag gtttcccgca tcaggaaatt
    45061 cgtcagtact ctttgactcg caaaccggat ggcaaaggct atcgtattgc ggtgaaacgc
    45121 gaagagggtg ggcaggtatc caactggctg cacaatcacg ccaatgttgg cgatgtcgtg
    45181 aaactggtcg ctccggcagg tgatttcttt atggctgtcg cagatgacac accagtgacg
    45241 ttaatctctg ccggtgtcgg tcaaacgcca atgctggcaa tgctcgacac gctggcaaaa
    45301 gcaggccaca cagcacaagt gaactggttc catgcggcag aaaatggcga tgttcacgcc
    45361 tttgccgatg aagttaagga actgggtcaa tcactgccac gctttaccgc acacacctgg
    45421 tatcgtcagc cgaatgaagc cgatcgcgct aaaggtcagt ttgatagcga aggtctgatg
    45481 gatttgagca aactggaagg tgcgttcagc gatccgacaa tgcagttcta tctctgcggc
    45541 ccggttggct tcatgcagtt tgccgcgaaa cagttagtgg atctgggcgt gaaacaggaa
    45601 aacattcatt acgaatgctt tggcccgcat aaggtgctgt gatttgatgt tgccggatgg
    45661 aaacatccgg caacccttga cccgttttaa atcgccgcat catcctcttc accggtacgg
    45721 atacgaatga cccgtgccac gtcgaagacg aagattttac cgtcaccgat tttgccggtt
    45781 tgcgccgtgc gaataatggt atcgacacag gtatcaacaa tgtcgtccgg tacgacaatc
    45841 tcaattttca ctttcggcag aaaatccacc atatactccg caccgcggta cagctcggta
    45901 tggcctttct ggcggccaaa acctttcacc tcagtgaccg tcatgccggt aataccgact
    45961 tcagccagtg cttcgcggac atcgtccagc ttgaagggtt taataatcgc atcaatcttt
    46021 ttcatgctat tccttgaaaa ggtcgcctgt cttttgatct gctaaacgta acacataacg
    46081 ccaattcatt ccttgaaatc gtttgcatcc agttcgtgtc gggaaagcag tttataaaat
    46141 tctgtccggt tgcgccccgc cattctcgcc gcgtgggtga cgttgccttt ggtgatttgc
    46201 agcagcttac gcaaatagtt gagttcaaac tgattacgcg cctcaacaaa ggttggcagc
    46261 gccgtgtttt cgccctccag cgcctgctcc accagcgcat cactaattac cggagatgat
    46321 gtcagcgcca cgcactgttc aatcacgttg accaactggc gcacgttgcc cggccagctc
    46381 gcagtcatca ggcgtttcat tgcatcggtg gagaacgcgc ggacaaacgg tttatgtcgc
    46441 tctgccgcct ggcgcaacag gtgatttgcc agtagcggaa tgtcttctgt gcgctccgcc
    46501 agggccggaa ttttcaggct gacaacgttg aggcggtaat agaggtcttc acggaattcc
    46561 ccgcgcgtca tcgcttttgg caggtcgcgg tgggtcgcag aaataatccg cacgtcgata
    46621 tcaatatcgc ggttactgcc cagcgggcgc actttacgct cctgcaacac gcgcagcagt
    46681 ttgacctgca acggtgcggg catatcgcca atctcatcga gaaatagcgt accgccttcc
    46741 gccgcctgga ataaaccttc gcgattgctg acagcgccag taaacgcgcc acgtgcatga
    46801 ccaaacagct ccgactccag caattgctcg ggtaatgcgc cacagttaat agcaataaat
    46861 ggcttgctgt tgcgcgggct ggcgttgtgg atagcctggg cgaaaatctc tttcccggtg
    46921 ccgctctggc cgttaatcaa aacgctaacg tctgattgtg ccaccagccg cgcctgttcc
    46981 agcaagcgca gcatcagcgg gctgcgggtg acaatcgcct cgcgccaacg ttcgtcggtg
    47041 gctggcgcgg attgttccag cgcatcgtca attgcctgat atagcgcatc tttatcgaca
    47101 ggcttggtga gaaaactgaa cacgccctgc tgtgttgcag caacggcatc aggaatagaa
    47161 ccatgcgcgg taagaataat tactggcatt cccggctgca ctttctgaat ttcagcaaat
    47221 agctgcatac catccatttc atccatccgc aggtcgctga tgactaaatc tactttttcg
    47281 cgattcagta cccgtaatcc ttcaacgcca ctttccgccg tgaccacgct gtagccttcg
    47341 ctggtcaggc gcaggccaag cagtttcagc aatcccggat catcatcgac cagtaataaa
    47401 tgcgcaggtt tatggctcat caggagtgac ctcatcggtg gatggcgcgg gcttttcact
    47461 ctcgtgcggc gtatccggcg agaaatttcc agccggtttg cgggtcgaga gctggcgttc
    47521 aatatcggtc aggttttcca gcttgcgggt agtgagttcc agttgctgtt gtagaacgtg
    47581 atgttgctgg cgcaaagtat ccagctcgct gtcgctggac tgctggagtt tgctgtagcg
    47641 ttggcgctct tccgccagtt gcagttgcag cgcctggccg tcgcgccaga gttgatacag
    47701 tgggcgaacc tgtgccggga tctcggtact tagcgcatca atgcgcgcga ccagctggcg
    47761 gcgctcatac ggcgtaattt tggcgtcggc gagcaaaatc ccttgtttaa aggtattttg
    47821 ccagttgccg tcgtcatatt ggcgggcttg ctgacgcgac tgcgcaggca ttaaacgatc
    47881 agcacaatcc atcgcccgca gccagtaaag cggattggtt tcggttgatt taccttgcag
    47941 cgcccagatg tcgctacatt cagtagaaag atagtcagcc agttgataaa ccggaatttt
    48001 ttcttctgct ggcgtatcaa tggctggctt attgtgactc tgcacgcaac ccagcaatgc
    48061 cagacatggc agccctgcca gccacagccg tcggggcaat aatcgttgaa aaatgtgtcg
    48121 catattcacc agacttaaag cctatcccag tgggcgtaat tgttgcaggc agtttggaca
    48181 tggacagcgc ggagaaacca gagcgtacat gtcgtacgtg aagatttcga gcactgcccg
    48241 gggccgaact gacaaataaa atagcctgat gggatgggct gttagcatta tttcgtgttt
    48301 ttcgacgacg gtaattcaat gcggaaacaa acgtcttgcc cgctctcgtc gaccagatac
    48361 agttcccctt gcatacggcg aatacaatcc ctggcaatgc ttaatcccag accgctgccc
    48421 ttcaccgccc cttttcgctg gtggcttccc tgaaaaaagg gttcgaagat catggcgcgt
    48481 tcctcttgcg gaatgggcgt gcctgtattg atgacatcaa tataaacccg cgcaccatgt
    48541 aaactgctgc gaaggcaaat gttaccggat tcagccccgt agtgcaccgc attggagtaa
    48601 agattatcca gtacgctcat cagcagcatt ggcttcgcca ggcaagctgt tgctttgaga
    48661 tcaacgtcgg tatgcatcat tttagcccgt gcgggcaggc tatgagcaga aaccactgtc
    48721 tccaccagcg gtgctaactc aacattctcc agttccaccg cactgtccgc ctgtttacgg
    48781 ttgtaatcaa gcagttgttc gatcagcttt tgcaaattgc ggctgctgct atcaagaatg
    48841 ctcaccacct ctttttgctc tggcgtaagc ggcccgacaa cctgatcagc cagtaattca
    48901 gtgccctcgc gcatactcgc cagtggcgtt tttaattcat gagataaatg tcttaaaaat
    48961 tgatggcgtt gggattccag ccatgacagg cgctcactta accaaagaat acgttgccca
    49021 accgagcgta actcgctcgg tccactgaac gagacgctat tgcccagaga acgcccttcc
    49081 cccagccggt tgatcatgcg ctcgatattt ttcaccggac cgataatcat cctcgtgaaa
    49141 agcagtacca tcaccagact caccagaaat agcaccagcg attgccaacc aaaatattgc
    49201 ccacgttcgg cgatttcacg ctgaagttgc tgcccacgag agaacaccac tgtgcgcgtg
    49261 gcctgcacca tttcggtatt ggcactggca aaggcttcca gacgcgcggc ggcagcagca
    49321 tcgggaccgc tgttgttaca ctgaagttga gccagattgt tcaaatcctg acgtaatgcc
    49381 tggtagagtt tatcgtccgg cagcacgcct gcgtgggcat cgagcatttc gctgtaacgc
    49441 ttgcgctggc tttgataaac cttcgcaagc gtagggtcgt ccagcacgca gtactgacga
    49501 tagctgcgct ccatctccag tgccgcgttg gtcatcgctt cactgcgccg ggcatcaata
    49561 agcgtagtgc ggttaaccag cgccgcctga tcgctaagtg cattcaggct ttgccaggct
    49621 tgccatgcca gcaccaacag gggcagcaga atcagcaaaa atgccagcat taccagttgt
    49681 cgtaatgagc ggggaaaaac gggccagcgt ttcaaggcgt tactctcgtc agacgcgaat
    49741 agcctgatgc taaccgaggg gaagttcaga tacaacaaag ccgggaatta cccggctttg
    49801 ttacggaata aggcggtgcc taactcgacg tttcgcccga tggttgatat agctacgctg
    49861 atatcagaag ttggacggca ggcaccttgt tgtgcgtcat tcgaatttta tgtagcacgt
    49921 cccgaagggg ctgacataag tcggcgaatg agccactgct tactattatg cagtaactgt
    49981 gccaataaag aaaatagttt ggtaacatac tgattatacg ttgctttgag gggtttatgg
    50041 cttctccgct atttgaatgt aaatcagcca ctgtgtcgct aaaaagagac aacttaagat
    50101 aaacttatta gataatatta aaatcaatga gttaagtgtc accaaaaagc gacacggcaa
    50161 accacccatt gtcgtgattt acagacacaa aaaagctccc gaagttggga gctcataata
    50221 gcggttggtg cttaatgccg gatgcggcgt gaatgcctca tccgggctgc aataccaatg
    50281 ggttgacgac ttaccccaac tgcttacgtg cattgcggaa aatgcgtatc catgggccat
    50341 cctcgcccca gttttccgga tgccaggagt tgctgacagt acggaaaact cgttccgggt
    50401 gcggcatcat aatggtgact cgaccacttt cagtcgtgac cgccgtaata ccgttcggtg
    50461 aaccgttcgg gttagccggg taggtttcag tgactttgcc gaagttatcg acatagcgca
    50521 gtgccaccag ccctttgctt tccagtgccg ccagatgcgc cgcatcgcgc acttcaacgc
    50581 gcccttcacc gtgagagaca gcaatcggca tctgcgagcc caccatcccc tgcaacagca
    50641 gcgacgggct ttgggttact tctaccagac tgaaacgcgc ttcaaagcga tcggaggtgt
    50701 tacgcacaaa acgtggccac aactcgctac ctgggatcag ttcacgcaga ttagacatca
    50761 tctggcaacc gttacatacc cccagcgcca gcgtttgcgg acgatggaag aaggttgcaa
    50821 actcatcgcg tacacggtca ttgaacagga tagacttcgc ccaaccttca ccagcgccca
    50881 gcacatcacc gtaggaaaaa ccaccgcacg ctaccaggac gtggaaaccc tccaggcccg
    50941 tgcgtccagc cagcagatcg ctcatatgca cgtcgatagc atcgaagccc gcacggtgga
    51001 aagctgccgc catttcaaca tgggagttca caccctgctc acgcagtacg gcaactttcg
    51061 ggcgcgcgcc agtggcaata aacggtgctg ccacatcttc gttgatatca aacgacagtt
    51121 tcacattcag gcccggatcg gcgtcgtttg atttcgcctg gtgttcctga tcggcacact
    51181 ccgggttgtc acgcagacgc tgcatctgcc aggtagtttc tgcccaccag acacgcaacg
    51241 tggtgcggct ttcgctaaat acagtctgcc cgttggcggt aatcacaaaa cggtcaccgg
    51301 aaaccgcctg cccaacataa tggacgcaat cagcaagccc atgctgtgcc agtaccgctt
    51361 caacggcttc acggtcagcg gctcgaacct gaatcaccgc acccagttct tcgctaaaca
    51421 atgcagccag gcgatcgtca cccagagtgg cgatatccgc gtcaatgcca caatgaccag
    51481 caaaggccat ttccgccagc gttaccagca ggccgccatc agagcggtcg tgatacgcca
    51541 gcagcttacg ctgtgcaacc agcgcctgaa tcgcgtcata gaagcctttc agttgggcaa
    51601 catcgcgtac atctgccggt ttgtcgccaa gctgacgata aacctgcgcc agtgccgttg
    51661 cgcccagcgc gttattgcct ttgcccaaat caatcagcaa cagtgcgtta tcttcggtag
    51721 aaagctgcgg cgtaatggtg tgacgtacat cttccacgcg ggcaaatgcg gaaatcacca
    51781 gcgacaacgg cgacgtcatt tcgcgctctt cgttaccttc ctgccagcgg gttttcatcg
    51841 acatagagtc tttacccacc ggtatcgtca ggcccagcgc cggacaaagt tcttcgccca
    51901 cagctttaac ggcttcatac aggcccgcat cttcaccagg gtggcctgcc gccgccatcc
    51961 agttggcgga aagtttaatg cgtttgatat cgccaatttg tgttgcggcg atgttggtta
    52021 gcgcttcacc gactgccaga cgggcagaag cggcgaaatc cagcagcgca actggcgcac
    52081 gttcgcctat tgccatcgct tcaccgtagt agctgtcgag gctggcggta gtgactgcgc
    52141 agttagcgac cggcacctgc cacggaccca ccatctgatc gcgcgccacc atgccggtta
    52201 cgctgcgatc gccgatggtc accagaaagg ttttctccgc cacagtcggc agatgtaaca
    52261 cacgtctcac cgcgtcagcg atggtgatcc cttcacgggc cagcgcgtcg cctttcgctt
    52321 tcagcgtttg cacatcgcgg gtcatcttcg gcgttttgcc aagcagtacg tcgagcggca
    52381 gatcgatggg ttgattatca aaatgacgat cgtgcagaga aagatgtaat tcttcggtgg
    52441 cctcaccaat caccgcgtac ggtgcgcgct cacgcttaca cagttcgtca aacagcggta
    52501 actgatcggc agcaactgcc agaacgtagc gttcctggga ttcgttacac cagatttcca
    52561 gcgggctcat gcccggttcg tcgctcagga tatcgcgcag ttcaaattta ccgccgcgtc
    52621 cgccgtcgct caccagctcc ggcatggcgt tagaaagacc gcctgcgccg acgtcgtgga
    52681 taaacagaat cgggttcgcg tcgacaagct gccagcagcg gtcgatcact tcctgacagc
    52741 gacgttccat ttccgggtta tcgcgctgaa cggacgcgaa atcgagatcc gcgtcagact
    52801 ggccggaggc catggaagag gccgcgccgc cgccaagacc gatgttcatt gcggggccgc
    52861 cgagaacgac cagtttcgca ccgacgttga tctcgccttt ttgtacgtga tcggcgcgaa
    52921 tgttgccgat cccacccgcc agcatgatcg gtttgtgata cccgcgcagc tcttcgccgt
    52981 tgtggctgtt cactttttct tcataggtac ggaagtagcc gttcagtgcc ggacgaccaa
    53041 attcgttgtt aaatgccgcg ccgcccagcg ggccttcggt catgatatcc agcgcagtga
    53101 caatgcgctc aggcttaccg aaatcttctt cccacggttg ttcaaagcca ggaatacgca
    53161 ggttggaaac ggagaaacca accaggccag ctttcggctt cgcgccgcgg ccggtggcac
    53221 cttcatcgcg gatttcaccg ccggaaccgg tagctgcgcc cggccacgga gaaatcgccg
    53281 ttgggtggtt gtgggtttct actttcatca ggatgtgcgc cggttcctga tggaagtcgt
    53341 agcggcccgt ttcgtggtca gcaaagtagc ggcccacttc ggaaccttcc attacagcgg
    53401 cgttatcttt ataagcagag agaacgtgat ccggcgtggt ttcgaaggtg tttttgatca
    53461 tcttaaacag cgatttcggc tgctgttcac catcgataac ccagtcggcg ttaaaaattt
    53521 tgtggcggca gtgctcggag ttcgcctggg caaacatata cagttcgatg tcgttcgggt
    53581 tacgaccaag ctttgtgaac gcatcctgca gatagtcaat ttcatcttct gccagtgcca
    53641 ggccaagacg caggttagcg tcgattagcg cctgacggcc ctgccccagc aaatcaacgc
    53701 tggtgaccgg agtcggttga tggtgtgcaa acagctgctc tgcatcatct aaagcaaaaa
    53761 agaccgtttc catcatgcgg tcgtgcagtt cagcggtaac ctgctgccat tgttcattgg
    53821 tcagcgtacc ggcttctata tagtaagcaa cgccgcgctc aaggcggttt acctgttgta
    53881 gaccgcaatt atgggcgata tcggtcgctt tcgaagacca gggagagatg gtgccaggac
    53941 gcggggtcac cagcaggagt ttgccttgcg gggcgtggct ggcgagtgcc gggccatatt
    54001 tcagcaggcg ttcaagttgt gcgtgctcat catcgtttaa cggcgcattg aggtcagcaa
    54061 aatggacata ctcggcgtaa atagtgtgaa ccgggagcct ggcagcctga aaacgtgcca
    54121 gcagtttgtt gattcggaat gccgacaatg caggcgaacc acgcagaatt tccatcataa
    54181 gtctctcgtc ttctaagctt tcggtgtacc caaggggggg aaacgggcgt cattataaag
    54241 aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa
    54301 cgcgtgccgc caatggctgt aataagttgc catctggcgc aggtttacgc aaaatgccgc
    54361 tcatttatga gtaaaccttt cactattatt acgttttttc aagctgggac gcgcacgaca
    54421 cagagagtta actaattgaa aaaattaaag attaattatc tgttcatcgg cattctggca
    54481 ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc cgacaaccgt
    54541 atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac tcccctgact
    54601 tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa acagtttgcc
    54661 gattacctcg gtgtaaaact gaaagtgacc gtgcggcaga atatcagcca gctgtttgac
    54721 gacctcgata atggtaacgc cgacctgctg gcggcaggac tggtctataa cagtgagcgg
    54781 gtaaaaaatt atcagcctgg tcctacctat tattccgtgt cacaacaact ggtttataaa
    54841 gtcggtcagt atcgcccacg tacgctgggc aacctgacgg cggagcaact caccgttgca
    54901 ccgggtcacg tggtggttaa cgatctccag accctgaaag acacaaaata cccggaatta
    54961 agctggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt catcgaagga
    55021 aaactcgatt acaccattgc tgattctgtc gccatcagcc tgtttcagcg cgttcacccg
    55081 gaactcgccg tagcgctcga tatcaccgat gaacaaccgg tgacctggtt tagcccgtta
    55141 gatggcgata ataccctttc cgccgccctg ctcgacttct tcaacgaaat gaatgaagac
    55201 ggtacactgg cacgcattga agagaaatac ctggggcatg gcgatgattt tgattacgtc
    55261 gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt
    55321 gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta tcaggaatcg
    55381 cactgggatg cgcaggccac ttcaccgacg ggtgtgcgcg gcatgatgat gctaaccaaa
    55441 aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag catcagcggc
    55501 ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa
    55561 cggatttggt ttgccctcgc cgcgtacaat atgggctatg cgcatatgct ggatgcccgc
    55621 gctctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa acagcgtctg
    55681 cctttactta gccagaaacc ctattacagc aagctgactt acggctacgc tcgtgggcat
    55741 gaagcctacg cttatgtcga aaatattcgt aagtatcaga ttagcctggt gggttatctg
    55801 caagagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga ttatccggcg
    55861 gtatcgccta cggagttggg caaagagaaa tttccttttc tctcgtttct ttcccagtcg
    55921 tcatcaaact atttgaccca ctctccctct ctgctgtttt ccagaaaagg gagtgaagag
    55981 aaacaaaatt aatctgtcga ggattgcgct tttttctgcg ctttaatttc ctggcggcgc
    56041 atgcgaaaga agtcactgag caacgccgca cactcatccg ccagtattcc ttccgtaatt
    56101 tccacccggt gattcatacc cggatgatgc agcacatcca ttaaagatcc cgcagcgcca
    56161 gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc gactgtggat catcgctccg
    56221 gcacacatta cacagggttc aagcgtgaca tacaacgtgg cgtcgatcag acgataattt
    56281 tgcatcacca gaccgccctg ccgtagggcc atgatttctg catgtgcggt gggatcatgt
    56341 cgaccaatcg gacggttcca gccttcgcca ataacacgat tgttatgcac taataccgcg
    56401 ccgaccggta cttcccgctc atcccaggca cgcttcgcca gcgtcattgc atgacgcatc
    56461 cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg
    56521 cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc accgcgcgga
    56581 gttactcgcc aacgatgctg acagaaataa agcagcgggt tgtcctgatt actgtcgcta
    56641 tagccactgt acagccgcag cggcgtgccg attttgcgct ccagttgcgc gaccttttca
    56701 tgtcccagac aacgcatcgt caatacccaa ccaccatagc cacgctgaat ctgactggcg
    56761 ataagattaa cccgcggcag ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc
    56821 ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt ggttaatcgc
    56881 tcctgaacca gcggaaaggc ggtaacattg tcacgaaacc agcgtacgaa atcggcctgc
    56941 aacgcctgca aacgcgcttc attgtgacca aaagtgcatc cccacagaag cagactcatc
    57001 ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg ctatgatcgg caacaacggc
    57061 agaacaagta acgcattcag cggttggcga cgcagtaaat aacgcaaaaa actgccgaac
    57121 atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt
    57181 gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact acatagccta
    57241 acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac tggaattttt
    57301 aattcatggc aattagcggc aatggaatat aaaattcact cgcgtgtgtc tcatattgcc
    57361 tgttgtcgcc attatttcat cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag
    57421 cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct gctacaacct
    57481 gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt cagtcagtcc
    57541 agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcact aaagttggcg
    57601 ttgagtgaag cgctggcaag ccagccggaa tctccctccg tgcccattca taaccaaatc
    57661 cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa tactgccgct
    57721 atgtacgcaa cgctaaacgt taatactgaa gagaaactgc atgaatgcgt aacaatgttg
    57781 cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt ggcgcaaaac
    57841 tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga tatgcatgcg
    57901 ctactcgcta cagtacaggc gtcgtcccct gacgatctgt tattagccat ttcctacacc
    57961 ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg cggaaaagtg
    58021 ctggcgatta ccggctttac tcccaatgcc ctgcaacagc gtgcttctca ttgcctgtat
    58081 accattgccg aagaacaagc gacaaacagt gcttcaatct ctgcttgtca cgcgcaggga
    58141 atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact ggcaccagaa
    58201 cgtattcgtc atagcgaagc gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata
    58261 atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgctgttaa tcactaaaaa
    58321 atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt cgatgggtga
    58381 acatatctac gagattaaca gcgataagtg taccgaatgc gtagggcact acgagacacc
    58441 aacctgccag aaggtgtgtc cgatccccaa tactattgtg aaagatccgc aacatgtcga
    58501 gacggaagaa cagttgtggg ataaatttgt gctgatgcac cacgcggata aaatttaata
    58561 ttctgctctg gaagtagagt gttaattatt ttatagaaaa atcttaacgc tattatattt
    58621 atttaaatga tgacattagc ataatcattc actaagttag tttatatagt atctgcccag
    58681 acacttattt atagttatta aaggcgcgtc cgattggttc accg
//
LOCUS       DAZGJT010000016        58038 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZGJT010000016 DAZGJT010000000
VERSION     DAZGJT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 58038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.016"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..640)
                     /locus_tag="ACHKFK_001110"
                     /pseudo
     CDS             complement(1..640)
                     /locus_tag="ACHKFK_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044502.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(768..943)
                     /locus_tag="ACHKFK_001111"
                     /pseudo
     CDS             complement(768..943)
                     /locus_tag="ACHKFK_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(1155..2000)
                     /gene="araC"
                     /locus_tag="ACHKFK_001112"
     CDS             complement(1155..2000)
                     /gene="araC"
                     /locus_tag="ACHKFK_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO9051500.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAHEWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCE"
     gene            2339..4039
                     /gene="araB"
                     /locus_tag="ACHKFK_001113"
     CDS             2339..4039
                     /gene="araB"
                     /locus_tag="ACHKFK_001113"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO9051501.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCATGEEIATSVEWYPRWQNGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCYTPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDNAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRILSWPLEQLAAQHPELKEQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            4050..5552
                     /gene="araA"
                     /locus_tag="ACHKFK_001114"
     CDS             4050..5552
                     /gene="araA"
                     /locus_tag="ACHKFK_001114"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO9051502.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            5837..6532
                     /gene="araD"
                     /locus_tag="ACHKFK_001115"
     CDS             5837..6532
                     /gene="araD"
                     /locus_tag="ACHKFK_001115"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9051503.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRGRGVLVIKPS
                     GVDYSIMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGINA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            6607..8958
                     /gene="polB"
                     /locus_tag="ACHKFK_001116"
     CDS             6607..8958
                     /gene="polB"
                     /locus_tag="ACHKFK_001116"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO9051504.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMRNGAIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASALDFELEYVASRPLLLEKLNAWFATHDPD
                     VIIGWNVVQFDLRMLQKHAERYRIPLRLGRDNSELEWREHGFKNDVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKTLIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFCNEPYHEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENHKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            9123..12029
                     /gene="rapA"
                     /locus_tag="ACHKFK_001117"
     CDS             9123..12029
                     /gene="rapA"
                     /locus_tag="ACHKFK_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO9051505.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQQGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            12041..12700
                     /gene="rluA"
                     /locus_tag="ACHKFK_001118"
     CDS             12041..12700
                     /gene="rluA"
                     /locus_tag="ACHKFK_001118"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO9051506.1"
                     /translation="MGMENYNPPQEPWLVVLYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(12818..13633)
                     /gene="djlA"
                     /locus_tag="ACHKFK_001119"
     CDS             complement(12818..13633)
                     /gene="djlA"
                     /locus_tag="ACHKFK_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO9051507.1"
                     /translation="MQYWGKIIGVTVALIMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            13888..16242
                     /gene="lptD"
                     /locus_tag="ACHKFK_001120"
     CDS             13888..16242
                     /gene="lptD"
                     /locus_tag="ACHKFK_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO9051508.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYKDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTRLAESANRVMPQFKVDGRMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSKIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISDRWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            16295..17581
                     /gene="surA"
                     /locus_tag="ACHKFK_001121"
     CDS             16295..17581
                     /gene="surA"
                     /locus_tag="ACHKFK_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO9051509.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            17581..18570
                     /gene="pdxA"
                     /locus_tag="ACHKFK_001122"
     CDS             17581..18570
                     /gene="pdxA"
                     /locus_tag="ACHKFK_001122"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO9051510.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLIVQLAQREWPVEQVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTVGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELREQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGKADVGSFITALNLAIKMIVNTQ"
     gene            18567..19388
                     /gene="rsmA"
                     /locus_tag="ACHKFK_001123"
     CDS             18567..19388
                     /gene="rsmA"
                     /locus_tag="ACHKFK_001123"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO9051511.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAA
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            19391..19768
                     /gene="apaG"
                     /locus_tag="ACHKFK_001124"
     CDS             19391..19768
                     /gene="apaG"
                     /locus_tag="ACHKFK_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO9051512.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            19775..20623
                     /gene="apaH"
                     /locus_tag="ACHKFK_001125"
     CDS             19775..20623
                     /gene="apaH"
                     /locus_tag="ACHKFK_001125"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO9051513.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYNIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDMGE
                     GEAVAS"
     gene            complement(20620..20934)
                     /locus_tag="ACHKFK_001126"
     CDS             complement(20620..20934)
                     /locus_tag="ACHKFK_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HFO9051514.1"
                     /translation="MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFS
                     RIRPPERLNPILLLVDGKEYVLMTHETATVPVNALGTKFCDASAHRTLIKGALDFMLD
                     GI"
     gene            complement(20937..21170)
                     /locus_tag="ACHKFK_001127"
     CDS             complement(20937..21170)
                     /locus_tag="ACHKFK_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFO9051515.1"
                     /translation="MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKH
                     AATRWREENAEAIAALNQLADETGCFSDEYRSF"
     gene            complement(21256..21735)
                     /gene="folA"
                     /locus_tag="ACHKFK_001128"
     CDS             complement(21256..21735)
                     /gene="folA"
                     /locus_tag="ACHKFK_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO9051516.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(21927..23789)
                     /gene="kefC"
                     /locus_tag="ACHKFK_001129"
     CDS             complement(21927..23789)
                     /gene="kefC"
                     /locus_tag="ACHKFK_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO9051517.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFVLSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLEKPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHGPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(23782..24312)
                     /gene="kefF"
                     /locus_tag="ACHKFK_001130"
     CDS             complement(23782..24312)
                     /gene="kefF"
                     /locus_tag="ACHKFK_001130"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO9051518.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(24420..25751)
                     /gene="yaaU"
                     /locus_tag="ACHKFK_001131"
     CDS             complement(24420..25751)
                     /gene="yaaU"
                     /locus_tag="ACHKFK_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051519.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(25810..26097)
                     /gene="fixX"
                     /locus_tag="ACHKFK_001132"
     CDS             complement(25810..26097)
                     /gene="fixX"
                     /locus_tag="ACHKFK_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO9051520.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(26094..27380)
                     /gene="fixC"
                     /locus_tag="ACHKFK_001133"
     CDS             complement(26094..27380)
                     /gene="fixC"
                     /locus_tag="ACHKFK_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9051521.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(27431..28372)
                     /gene="fixB"
                     /locus_tag="ACHKFK_001134"
     CDS             complement(27431..28372)
                     /gene="fixB"
                     /locus_tag="ACHKFK_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO9051522.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLSGKPDERMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LNAAVSNDASAVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDVAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(28387..29157)
                     /gene="fixA"
                     /locus_tag="ACHKFK_001135"
     CDS             complement(28387..29157)
                     /gene="fixA"
                     /locus_tag="ACHKFK_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO9051523.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     VLAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            29630..31131
                     /gene="caiT"
                     /locus_tag="ACHKFK_001136"
                     /pseudo
     CDS             29630..31131
                     /gene="caiT"
                     /locus_tag="ACHKFK_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
     gene            31162..32304
                     /gene="caiA"
                     /locus_tag="ACHKFK_001137"
     CDS             31162..32304
                     /gene="caiA"
                     /locus_tag="ACHKFK_001137"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO9051524.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            32433..33650
                     /gene="caiB"
                     /locus_tag="ACHKFK_001138"
     CDS             32433..33650
                     /gene="caiB"
                     /locus_tag="ACHKFK_001138"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO9051525.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMTKGKDPYYAGCGLYKCADGYIVMELVGITQIAECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            33723..35276
                     /gene="caiC"
                     /locus_tag="ACHKFK_001139"
     CDS             33723..35276
                     /gene="caiC"
                     /locus_tag="ACHKFK_001139"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFO9051526.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSSGVVNRYSYLELN
                     QEINRTANLFYVLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREEST
                     WILQNSQACLLVTSAQFYPMYQQIQQEDASQLRHICLTDMVLPADDGVSSFTQRKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHCLREVMFYLNLSEQEKDAFCERFSVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGELGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFCFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            35385..36170
                     /gene="caiD"
                     /locus_tag="ACHKFK_001140"
     CDS             35385..36170
                     /gene="caiD"
                     /locus_tag="ACHKFK_001140"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO9051527.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGVVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            36176..36766
                     /gene="caiE"
                     /locus_tag="ACHKFK_001141"
     CDS             36176..36766
                     /gene="caiE"
                     /locus_tag="ACHKFK_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO9051528.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFRGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(36885..37280)
                     /gene="caiF"
                     /locus_tag="ACHKFK_001142"
     CDS             complement(36885..37280)
                     /gene="caiF"
                     /locus_tag="ACHKFK_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO9051529.1"
                     /translation="MCEGCVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(37541..40762)
                     /gene="carB"
                     /locus_tag="ACHKFK_001143"
     CDS             complement(37541..40762)
                     /gene="carB"
                     /locus_tag="ACHKFK_001143"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO9051530.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHSMEEALAVAA
                     EVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLHAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTTIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(40780..41928)
                     /gene="carA"
                     /locus_tag="ACHKFK_001144"
     CDS             complement(40780..41928)
                     /gene="carA"
                     /locus_tag="ACHKFK_001144"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO9051531.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEPYSWTQGSWTLTGGLPEAKKEDELPYHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIALIEQYRKTAK"
     gene            complement(42384..43205)
                     /gene="dapB"
                     /locus_tag="ACHKFK_001145"
     CDS             complement(42384..43205)
                     /gene="dapB"
                     /locus_tag="ACHKFK_001145"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO9051532.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(43366..43731)
                     /locus_tag="ACHKFK_001146"
     CDS             complement(43366..43731)
                     /locus_tag="ACHKFK_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9051533.1"
                     /translation="MTYGEAYLEGWKNIFNYEGVSNRFEFWSFMIGSGVICLLPLLCW
                     WLAVTINNDYGVFIFFALPASFILTLIFAVPAIALAVRRMHDIGYSGWWVTIVVLIPV
                     TGVILLILCCLPSKSQDQA"
     gene            complement(43754..44668)
                     /gene="rihC"
                     /locus_tag="ACHKFK_001147"
     CDS             complement(43754..44668)
                     /gene="rihC"
                     /locus_tag="ACHKFK_001147"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO9051534.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYICRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLSELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAL"
     gene            complement(44734..45684)
                     /gene="ispH"
                     /locus_tag="ACHKFK_001148"
                     /gene_synonym="lytB"
     CDS             complement(44734..45684)
                     /gene="ispH"
                     /locus_tag="ACHKFK_001148"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO9051535.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDATDIQEEWVKEAKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(45686..46135)
                     /gene="fkpB"
                     /locus_tag="ACHKFK_001149"
     CDS             complement(45686..46135)
                     /gene="fkpB"
                     /locus_tag="ACHKFK_001149"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9051536.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(46243..46737)
                     /gene="lspA"
                     /locus_tag="ACHKFK_001150"
     CDS             complement(46243..46737)
                     /gene="lspA"
                     /locus_tag="ACHKFK_001150"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO9051537.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(46737..49553)
                     /gene="ileS"
                     /locus_tag="ACHKFK_001151"
     CDS             complement(46737..49553)
                     /gene="ileS"
                     /locus_tag="ACHKFK_001151"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO9051538.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIIKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKTKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVSDYTILGTVKGAELELLRFTHPFMDFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVIALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(49596..50537)
                     /gene="ribF"
                     /locus_tag="ACHKFK_001152"
     CDS             complement(49596..50537)
                     /gene="ribF"
                     /locus_tag="ACHKFK_001152"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO9051539.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(50545..50763)
                     /gene="yaaY"
                     /locus_tag="ACHKFK_001153"
     CDS             complement(50545..50763)
                     /gene="yaaY"
                     /locus_tag="ACHKFK_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO9051540.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSARKIAAGGQFLPMICAIAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            50866..51129
                     /gene="rpsT"
                     /locus_tag="ACHKFK_001154"
     CDS             50866..51129
                     /gene="rpsT"
                     /locus_tag="ACHKFK_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO9051541.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            complement(51225..52130)
                     /gene="nhaR"
                     /locus_tag="ACHKFK_001155"
     CDS             complement(51225..52130)
                     /gene="nhaR"
                     /locus_tag="ACHKFK_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO9051542.1"
                     /translation="MSMSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRAL
                     EERLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVA
                     DALSKRLVSSVLDAAVVEDEQIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQE
                     GLFSMKIGECGVSFWCTNPLPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNV
                     EILGEFDDAALMKAFGATHNAIFVAPSLYANDFYNDDSVVEIGRVENVMEEYHAIFAE
                     RMIQHPAVQRICNTDYSALFTPASK"
     gene            complement(52190..53356)
                     /gene="nhaA"
                     /locus_tag="ACHKFK_001156"
     CDS             complement(52190..53356)
                     /gene="nhaA"
                     /locus_tag="ACHKFK_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO9051543.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAVLAMIMANSGATSGWYHDFLET
                     PVQLRVGTLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLEGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            complement(53591..54850)
                     /locus_tag="ACHKFK_001157"
     CDS             complement(53591..54850)
                     /locus_tag="ACHKFK_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO9051544.1"
                     /translation="MFTNVNVDCCKTPGCKNLGLLNSQDYVAQGKNILCRECGYLFPV
                     ISEQSLNIYRNIVNHSWRGLICQCSTCGGTSLKKYGYSAQGQRRMYCHHCEKTFITLE
                     HVITTPRGAQLALMIEQGEALADIRKSLLLNSTGLSRELLKLAREANYKESRQCFPAS
                     DITLSTRDFRVKYNGSNNSLYALVTAEEQSGRVVAISTNYSPSAVEQHYQYTSNYEER
                     MSPGTLAHHVQRKELLTMRRDTLFDIDYGPAVLHQNDPGMLVKPVLPAYRHFELVRIL
                     TDEHSNNVQHYLDHECFILGGCLMANLQHIHQGRCHISFVKERGVAPATIDFPPRLFL
                     SGGVRNNVWRAFSNRNYSMAVCNLTGSKKVREMRHATLNSATRFIHFVENHPFLISLN
                     RMSPANVVSTLDILKHLWNKKLEHGTI"
     gene            complement(54979..56472)
                     /locus_tag="ACHKFK_001158"
     CDS             complement(54979..56472)
                     /locus_tag="ACHKFK_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9051545.1"
                     /translation="MQKTLMASLIGLAVCTGNAFSPALAAEAKQPNLVIIMADDLGYG
                     DLATYGHQIVKTPNIDRLAQEGVKFTDYYAPAPLSSPSRAGLLTGRMPFRTGIRSWIP
                     SGKDVALGRNELTIANLLKAQGYDTAMMGKLHLNAGGDRTDQPQAQDMGFDYSLVNTA
                     GFVTDATLDNAKERPRYGMVYPTGWLRNGQPTPRADKMSGEYVSSEVVNWLDNKKDSK
                     PFFLYVAFTEVHSPLASPKKYLDMYSQYMSAYQKQHPDLFYGDWADKPWRGVGEYYAN
                     ISYLDAQVGKVLDKIKAMGEEDNTIVIFTSDNGPVTREARKVYELNLAGETDGLRGRK
                     DNLWEGGIRVPAIIKYGKHLPQGMVSDTPVYGLDWMPTLAKMMNFKLPTDRTFDGESL
                     VPVLEQKALKREKPLIFGIDMPFQDDPTDEWAIRDGDWKMIIDRNNKPKYLYNLKSDR
                     YETLNLIGKEPDIEKQMYGKFLKYKTDIDNDSLMKARGDKPEAVTWG"
     gene            complement(56492..57253)
                     /locus_tag="ACHKFK_001159"
     CDS             complement(56492..57253)
                     /locus_tag="ACHKFK_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO9051546.1"
                     /translation="MSAKRRLLIACTLITAIYHFPAYSSLEYKGTFGSINAGYADWNS
                     GFVNTHRGEVWKVTADFGVNFKEAEFYSFYESNVLNHAVAGRNHTVSAMTHVRLFDSD
                     MTFFGKIYGQWDNSWGDDLDMFYGFGYLGWNGEWGFFKPYIGLHNQSGDYVSAKYGQT
                     NGWNGYVVGWTAVLPFTLFDEKFVLSNWNEIELDRNDAYTEQQFGRNGLNGGLTIAWK
                     FYPRWKASVTWRYFDNKLGYDGFGDQMIYMLGYDF"
     gene            57868..58020
                     /gene="mokC"
                     /locus_tag="ACHKFK_001160"
     CDS             57868..58020
                     /gene="mokC"
                     /locus_tag="ACHKFK_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO9051547.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT        14378 a        15509 c        14538 g        13613 t
ORIGIN      
        1 tataatttaa ccattctgga agcttctttt ttattagttg attttttaaa aggaggatgg
       61 gttgtaaata agaatggcgt aaactcagta tttttaaaat tcccttgcgc tccatatgta
      121 tcccatattt gtaaggcttt tgtgtaagat aaaggttttg ccaataaaag tggatcgggg
      181 cctgttccaa atacacgata aaaaccaatc aagtctgaga ctaaatcttc tgcactaaat
      241 ccgctatcag tataccagtt aaaaataaat gagttttgcc atgcgtcaaa cctcttcgca
      301 gttccataca ttaacgttaa ggcaactctt gctttaagag tgggtgaaag acatgaacga
      361 accataacct tcgtataaac accggtatga aatgtaagat ctactcctaa tattttttcc
      421 gaattcccat ttcctgatga taatcaacag gaaaatacca atcatgaaat tcatcgtaat
      481 atgttgaaaa atgttcttgc tccaattttc tttttaatat tctggcatct tgtccctgag
      541 catgccccaa atcgatccaa cctaactctt cggtataaac taatccatac tttttcgttg
      601 ttgcaaatat tccatcagta atatcgttac gtttgctcat cacaatctcc cctcaaaaaa
      661 aacgtatagc aaataatatc aataaggaaa aatattattt ttcaatattt ttttgtatac
      721 aattcatttt atgaatgaat ttacaaagat agctgcgcct gatgccgcta cacttttgcc
      781 agaccatgtt ctcctctgag ggagtaactg gcgtttttca tagaatgccg tacttttttc
      841 aacaactgcc acatcgaacg gcatttatgg tggcgtgaag tgcctgccat aaccgctcaa
      901 tgtggtgttc ctacgatgaa caaaccgctt gatatattct cctgatgcaa ccggacaata
      961 tgttcatgat gggtatcccg atacataagc cttcgtttta tttttacaaa aagaaatcgt
     1021 tgtcgatatt ttatcaagaa aaaaaataat tcaggtaaaa cgattcaact aaaaataact
     1081 aaaattaatc ccccgtgaaa tatataaata acgtcagatg aagaaagcaa gggggttccc
     1141 ccccttcttt cttattattc acaacctgcc ctaaactcgc tcggactcgc cccggtgcat
     1201 tttttaaata ctcgcgagaa atagagttga tcgtcaaaac cgacattgcg accgacggtg
     1261 gcgataggca tccgggtggt gctcaaaagc agcttcgcct gactgatgcg ctggtcctcg
     1321 cgccagctta atacgctaat ccctaactgc tggcggaaca aatgcgacag acgcgacggc
     1381 gacaggcaga catgctgtgc gacgctggcg atatcaaaat tactgtctgc caggtgatcg
     1441 ctgatgtact gacaagcctc gcgtacccga ttatccatcg gcggatgaag cgactcgtta
     1501 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag caattccgaa
     1561 tagcgccctt ccccttgtcc ggcattaatg atttgcccaa acaggtcgct gaaatgcggc
     1621 tggtgcgctt catccgggcg aaagaaaccg gtattggcaa atatcgacgg ccagttaagc
     1681 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgtgagcc
     1741 tccggatgac gaccgtagtg atgaatctct ccaggcggga acagcaaaat atcacccggt
     1801 cggcagacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagatta
     1861 agaatataac ccttcattcc cagcggtcga tcgataaaaa aatcgagata accgttggcc
     1921 tcaatcggcg ttaaacccgc caccagatgg gcgttaaacg agtatcccgg cagcagggga
     1981 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt
     2041 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaacccaa
     2101 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
     2161 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
     2221 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc agcctgacgc
     2281 tttttttcgc aactctctac tgtttctcca tacccgtttt tttggatgga gtgaaacgat
     2341 ggcgattgca attggcctcg attttggcag tgattctgtg cgagctttgg cggtggactg
     2401 cgccaccggt gaagagatcg ccaccagcgt agagtggtat ccccgttggc agaacgggca
     2461 attttgtgat gccccgaata accagttccg tcatcatccg cgtgactaca ttgagtcaat
     2521 ggaagcggcg ctgaaaactg tgcttgcaga gcttagcgtc gaacagcgcg cagctgtggt
     2581 cgggattggc gttgacacaa ccggctcgac gcccgcaccg attgacgccg acggtaacgt
     2641 gctggcgctg cgaccggagt ttgccgagaa cccgaacgcg atgttcgtat tgtggaaaga
     2701 ccacaccgcg gttgaagaag cggaagagat tacccgctta tgttacacgc cgggcaacgt
     2761 tgactactcc cgctatattg gcggtattta ttccagcgaa tggttctggg caaaaatcct
     2821 gcatgtaact cgccaggaca acgccgtggc acaatctgcc gcatcatgga ttgagctgtg
     2881 cgactgggtg ccagctctgc tttccggtac cacccgcccg caggatattc gtcgcggacg
     2941 ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg ggtggcctgc cgccagccag
     3001 tttctttgat gagctggacc caatcctcaa tcgccatttg ccttccccgc tgttcactga
     3061 tacctggact gccgatattc cggtgggcac cttatgcccg gaatgggcgc agcgtctcgg
     3121 cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac tgccatatgg gcgcagttgg
     3181 cgcaggcgca cagcctaacg cactggtaaa agttatcggt acttccacct gcgacattct
     3241 gattgccgac aaacagagcg ttggcgagcg ggcagtgaaa ggtatttgcg gtcaggttga
     3301 tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc caatcggcat ttggggatat
     3361 ctacgcctgg tttggacgca tactcagctg gccgctggaa cagcttgccg cccagcatcc
     3421 ggaactgaaa gagcaaatca acgccagcca gaaacaactg cttccggcgc tgaccgaagc
     3481 atgggccaaa aatccgtctc tggatcacct gccggtggtg ctcgactggt ttaacggtcg
     3541 ccgcacgcca aacgctaacc aacgcctgaa aggagtgatt accgatctga acctcgccac
     3601 cgacgctccg ctgctgttcg gcggtttaat tgccgccacc gcctttggcg cacgcgcaat
     3661 tatggagtgc ttcaccgatc aggggatcgc cgtcaataac gtgatggcgc tgggcggcat
     3721 cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac gtgctgaatc gcccgctgca
     3781 aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg atttttgctg ctgtcgccgc
     3841 gaaagtgcac gcagacatcc catcagccca gcaaaaaatg gccagtgcgg tagagaaaac
     3901 cctgcaaccg cgcagcgaac aggcacaacg ctttgaacag ctttatcgcc gctatcagca
     3961 atgggcgatg agcgccgaac aacactatct tccaacttcc gccccggcac aggctgccca
     4021 ggccgttccg actctataag gacacgacaa tgacgatttt tgataattat gaagtgtggt
     4081 ttgtaattgg cagccagcat ctttacggcc cggagactct gcgccaggtg acgcaacatg
     4141 cggaacacgt tgttaatgca ctgaatacag aagcgaagtt gccctgcaaa ctggtgctga
     4201 aaccgctggg caccacgccg gatgaaatca ccgctatttg ccgcgacgcg aattacgacg
     4261 atcgttgcgc cgggctggta gtgtggctgc acactttctc cccggccaaa atgtggatca
     4321 acggcctgac catgctcaac aaaccgttgc tgcaattcca cacccagttc aacgcggcgc
     4381 tgccgtggga cagcatcgac atggacttta tgaacctgaa ccagaccgcg catggcggtc
     4441 gcgagttcgg cttcatcggc gcgcgtatgc gtcaacaaca tgccgtcgtt accggtcact
     4501 ggcaggataa acaagcacat gagcgtatcg gctcctggat gcgtcaggcg gtctctaaac
     4561 aggatacccg tcatctgaaa gtctgccgtt ttggcgataa catgcgtgaa gtagcggtca
     4621 ccgatggcga taaagttgcc gcacagatca agttcggttt ctccgtcaat acctgggcgg
     4681 ttggcgatct ggtgcaggtg gtgaactcca tcagcgacgg cgatgttaac gcgctagtcg
     4741 atgagtacga aagctgctac accatgactc ctgccacaca aatccacggc gaaaaacgac
     4801 agaacgtgct ggaagcggcg cgtattgagc tggggatgaa gcgtttcctg gaacaaggtg
     4861 gcttccacgc tttcactact acctttgaag atttgcacgg cctgaaacag cttccgggtc
     4921 tggccgtaca gcgtctgatg cagcagggct acggctttgc gggcgaaggc gactggaaaa
     4981 ctgccgccct gcttcgcatc atgaaggtga tgtcaaccgg tctgcagggc ggcacctcct
     5041 ttatggagga ctacacctat cacttcgaga aaggtaatga cctggtactc ggctcccata
     5101 tgctggaagt ctgtccgtcg atcgctgtgg aagagaaacc gatcctcgac gttcagcatc
     5161 tcggtattgg cggtaaagac gatcctgccc gcctgatctt caacactcaa accggtccag
     5221 ccattgtcgc cagcctgatt gatctcggcg atcgttaccg tctgctggtt aactgcatcg
     5281 acacggtgaa aacaccgcac tccctgccga aactgccggt ggcgaatgcg ctgtggaaag
     5341 cgcaaccgga tctgccgacc gcttccgaag cgtggatcct tgccggtggc gcgcaccata
     5401 ccgtcttcag ccatgcgctg aacctcaacg atatgcgcca gttcgccgag atgcacgaca
     5461 ttgaaatcac agtgattgat aacgataccc gccttccagc gtttaaagac gcgctgcgct
     5521 ggaacgaagt gtattacgga tttcgtcgct aagtagccgc atcaggtatg taacgcctga
     5581 tgcgacgctg acgcgtctta tcaggcctac gcgttccgga tttgtaggcc ggataagtaa
     5641 agcgcatccg gcattcaccg cctgatgcgg cgctggcgcg tcttatcagg cctacgcgtt
     5701 ccggatttgt aggccggata agcaaagcgc atccggcatt caccgcctga tgcgacgccg
     5761 acgcgtctta tcaggcctac acgctgcgat tttgtaggcc ggataagcaa agcgcatccg
     5821 gcacgaagga gtcaacatgt tagaagatct caaacgccag gtattagaag ccaacctggc
     5881 gctgccaaaa cacaacctgg tcacgctcac ctggggcaat gtcagcgccg ttgatcgcgg
     5941 gcgcggcgtc ctggtgatca aaccttccgg cgtcgactac agcatcatga ccgctgacga
     6001 tatggtcgtg gtcagcatcg aaaccggtga agtggttgaa ggtacgaaaa agccctcctc
     6061 cgacacgcca actcaccggc tgctctatca ggcattcccg tctattggcg gcattgtgca
     6121 cacacactcg cgccacgcca ccatctgggc gcaggcgggt cagtcgattc cagcaaccgg
     6181 taccacccac gccgactatt tctacggcac cattccctgc acccgcaaaa tgaccgacgc
     6241 agaaatcaac ggtgaatatg agtgggaaac cggtaacgtc atcgtagaaa ccttcgaaaa
     6301 acagggtatc aatgcagcgc aaatgcccgg cgtgctggtc cattctcacg gcccgtttgc
     6361 atggggcaaa aatgccgaag atgcggtgca caacgccatc gtgctggaag aagtcgctta
     6421 tatggggatt ttctgccgcc agcttgctcc gcagttaccg gatatgcagc aaacgttgct
     6481 ggataagcac tatctgcgta agcatggcgc aaaagcctat tacggccagt aatgactgta
     6541 taaaaccaca gccagtcaac caaaaccagg ctatactcaa gcctggtttt ttattggatt
     6601 ttcagcgtgg cgcaggcagg ttttatctta acccgacact ggcgggacac cccgcaagga
     6661 accgaagttt ccttttggtt agcgacggac aacgggccgt tgcaggttac gcttgcgccg
     6721 caagagtccg tggcgtttat tcccgccgat caggttcccc gcgcccagca tattttgcag
     6781 ggtgagcaag gctttcgcct gacgccgctg gcgttaaagg attttcatcg ccagccggtg
     6841 tatggccttt actgtcgcgc ccatcgccag ttgatgaatt acgaaaagcg cctgcgtgaa
     6901 ggtggcgtta ccgtctacga ggccgatgtg cgcccgccag aacgctatct gatggagcgg
     6961 tttatcacct cgccagtgtg ggtcgagggt gatatgcgca acggcgctat cgttaatgcc
     7021 cgtctgaaac cgcatcccga ctatcgtccg ccgctcaagt gggtttctat tgatattgaa
     7081 accacccgcc acggtgagct gtactgcatc ggcctggaag gttgcgggca gcgtatcgtt
     7141 tatatgctgg ggccagagaa tggcgacgcc tccgcgctcg attttgaact ggaatacgtc
     7201 gccagccgcc cgctgctact ggaaaaactt aacgcctggt ttgcgactca tgatcctgat
     7261 gtgatcatcg gttggaacgt ggtgcagttc gatctgcgaa tgctgcaaaa acatgccgag
     7321 cgttaccgta ttccgctgcg tctggggcgt gataacagcg agctggagtg gcgcgagcac
     7381 ggctttaaaa acgacgtctt ttttgctcag gccaaagggc gactgattat cgacggtatc
     7441 gaggcgctga aatccgcgtt ctggaatttc tcttcattct cgctggaaac cgtcgctcag
     7501 gagttattag gcgaaggaaa atctatcgat aacccgtggg atcgaatgga cgaaattgac
     7561 cgccgtttcg ccgaagataa acctgcgctg gcaacttata acctgaaaga ttgcgagctg
     7621 gtgacgcaga tcttccacaa aactgaaatc atgccgtttt tgctcgaacg ggcgacggtg
     7681 aacggcctgc cggtagatcg acacggcggt tcggtggcag cgtttggtca tctctatttt
     7741 ccccgtatgc atcgcgctgg ttatgtcgcg cctaatctcg gcgaagtgcc gccgcacgcc
     7801 agccctggcg gctacgtgat ggattcgcga cctgggcttt atgattcggt gctggtgctg
     7861 gactacaaaa gcctgtaccc gtcgatcatc cgcacctttc tgattgatcc cgtcgggctg
     7921 gtggaaggca tggcgcagcc tgatccagag cacagtaccg aaggttttct cgatgcctgg
     7981 ttctcacggg agaaacattg cctgccggag attgtgacta acatctggca cgggcgcgat
     8041 gaagccaaac gccagggtaa taagccgctt tcgcaggcgc tgaagatcat tatgaatgcc
     8101 ttttatggtg tgctcggcac caccgcctgc cgtttcttcg atccacgcct ggcatcgtcg
     8161 atcaccatgc gtggtcatca gatcatgcgg caaaccaaaa cgttgattga agcgcagggc
     8221 tatgatgtta tctacggcga taccgactca acgtttgtct ggctgaaagg tgcacattcg
     8281 gaagaagaag cagcgaaaat cggtcgtgta ctggtgcagc acgttaacgc ctggtgggcg
     8341 gaaacgctgc aaaaacaacg gctgaccagc gcactggaac tggagtatga aacccatttc
     8401 tgccgttttc tgatgccaac cattcgcggg gccgataccg gcagcaaaaa gcgctacgcc
     8461 ggactgattc aggagggcga caaacagcgg atggtgttta aagggctgga aaccgtgcgt
     8521 acggactgga cgccgctggc ccagcagttt cagcaggagc tttacctgcg catcttctgc
     8581 aacgagccat atcatgaata cgtgcgcgaa accatcgaca agctgatggc gggcgaactg
     8641 gatgcgcggc tggtttaccg taaacgcctt cgccgtccgc tgagcgaata tcagcgtaat
     8701 gttccgcctc atgtacgcgc cgctcgcctt gccgatgaag aaaaccataa gcgcggtcgc
     8761 cccttgcaat atcagaaccg cggcaccatt aagtacgtat ggaccaccaa cggcccggag
     8821 ccgctggact accaacgttc accactggat tacgaacact atctgacccg ccagctacaa
     8881 cccgtggcgg agggaatact cccttttatt gaggataatt ttgctacact tatgaccggg
     8941 caacttgggc tattttgagc aaaaaaaaga gttcgccaga taccattttg atgcgtgacg
     9001 aatgctttgc catccagtac catagcgccc tttccattcc tggacctgaa taacaccact
     9061 acctcataag cacggtagcg ggtggttatt gcctgcaatt aaagatatag agccgaacac
     9121 atatgccttt tacacttggt caacgctgga tcagcgatac agaaagcgaa ttgggacttg
     9181 gaaccgttgt cgcggtggat gcgcgaactg tcactttact tttcccatct actggtgaaa
     9241 accgtctgta cgcacgcagt gattcccccg tgacccgcgt gatgttcaac cctggtgata
     9301 ccattaccag ccatgacggc tggcagatgc aagtcgaaga agtaaaagaa gaaaatggct
     9361 tgctgaccta tatcggtact cgccttgata ctgaagagtc cggcgtagcc ctgcgtgaag
     9421 ttttccttga tagcaaactg gtgttcagca aaccgcagga ccgtctgttt gccgggcaga
     9481 ttgaccgtat ggaccgcttt gcgctgcgtt atcgcgcgcg taagtattcc agcgaacagt
     9541 tccgtatgcc gtacagcggc ctgcgcggtc agcgtaccag cctgatcccg caccagctca
     9601 acatcgctca tgatgttggt cgccgccacg cgccgcgcgt cctgctggct gacgaagtgg
     9661 gtttagggaa aaccattgaa gccgggatga tcctgcatca gcaactgctc tctggcgctg
     9721 ctgaacgtgt gctgattatc gtcccggaaa ccttacagca tcagtggctg gtagaaatgc
     9781 tgcgccgttt caacctgcgc tttgcgctat ttgatgatga gcgttatgcc gaagctcagc
     9841 acgatgctta caacccgttc gacaccgaac agctggtgat ttgctcgctg gattttgccc
     9901 gtcgtagcaa acagcgtctg gaacatctct gtgaagccga gtgggacctg ctggtggtcg
     9961 atgaagcgca tcacctggtg tggagcgaag atgcgccgag ccgtgaatat caggccattg
    10021 aacaactggc agagcacgtg cccggcgttc tgctgctgac cgcaaccccg gaacaactgg
    10081 ggatggaaag ccacttcgcc cgtctgcgtc tgctggaccc gaaccgtttc cacgattttg
    10141 cgcaattcgt tgaagagcag aaaaattatc gtccggttgc ggacgccgtt gccatgctgc
    10201 tggctggtaa caaactcagc aatgacgaac tgaacatgct cggtgagatg atcggcgagc
    10261 aggatatcga gccgctgttg caagcagcaa acagcgacag cgaagatgcc cagagcgccc
    10321 gtcaggagct ggtttcgatg ctgatggatc gccacggcac cagccgcgtg ctgttccgta
    10381 acacccgtaa cggtgtgaaa ggcttcccga aacgcgagct gcacaccatt aagctgccgc
    10441 tgccgacgca gtatcagacg gctattaaag tctccggcat tatgggcgca cgcaaaagtg
    10501 cggaagatcg cgctcgcgat atgctctacc cggagcgtat ttatcaggaa tttgaaggtg
    10561 ataacgccac ctggtggaac ttcgatccgc gcgttgagtg gctgatgggc tacctgacca
    10621 gccatcgctc tcagaaagtg ctggtgatct gcgccaaagc tgccactgcg ctgcaactgg
    10681 agcaggtact gcgagaacgt gaaggtattc gcgccgcggt attccacgaa ggtatgtcga
    10741 ttatcgaacg tgaccgcgct gccgcctggt ttgccgaaga agacaccggc gcgcaggtac
    10801 tgctgtgctc ggaaatcggt tctgaaggac gtaacttcca gttcgccagc cacatggtaa
    10861 tgtttgacct gccattcaac ccggatctgc tggagcagcg tattgggcgt ctggatcgta
    10921 tcggtcaggc gcacgatatt cagatccatg tgccttatct ggaaaaaact gctcagtcgg
    10981 tgctggtgcg ctggtatcac gaaggtctgg atgcattcga gcacacctgc ccgaccggac
    11041 gcactattta cgatagcgta tacaacgatc tgattaacta tctggcttca ccggatcaaa
    11101 ccgaaggctt tgacgatctg atcaaaaact gccgcgagca acatgaagcg ctgaaagcac
    11161 agctggaaca aggtcgtgac cgcctgctgg aaatccattc caacggtggc gaaaaagccc
    11221 aggcactggc agaaagcatt gaagagcagg atgacgatac caacctgatc gccttcgcca
    11281 tgaacctgtt cgatattatc ggtatcaatc aggacgatcg cggcgacaac atgatcgtgc
    11341 tgacgccgtc cgatcatatg ctggtgccgg acttccctgg cctgtcggaa gatggcatca
    11401 ccatcacctt cgaccgtgaa gtggcgctgg cgcgtgaaga tgcacagttt attacctggg
    11461 agcatccgct gatccgcaac ggtctggatc tgatcctttc tggcgatacc ggtagcagca
    11521 cgatttcact gttgaaaaac aaagcgttgc cggtaggtac gctgttggtg gaactgattt
    11581 atgtggttga agcccaggct ccgaagcagt tgcagctcaa ccgcttcctg ccaccgacgc
    11641 cggtacgtat gctgctggat aaaaacggca acaacctggc ggcgcaggta gagtttgaaa
    11701 cctttaaccg ccagcttaac gcggttaacc gtcacaccgg cagcaagctg gttaacgcgg
    11761 tgcagcagga tgttcacgct atccttcagc agggggaagc gcagatcgag aaatctgccc
    11821 gtgcattgat tgatgcagcg cgtaacgaag ccgacgaaaa actgtctgcc gagctgtctc
    11881 gtctggaagc gctgcgtgca gttaacccga acattcgtga cgacgaactg accgccattg
    11941 agagcaaccg tcagcaggta atggaaagcc tggatcaggc aggctggcgt ctggatgccc
    12001 tgcgtttgat cgttgtgacg catcagtaac ggagccgaag atggggatgg aaaactacaa
    12061 tccgccgcag gaaccctggc tggttgtcct gtatcaggat gaccatatta tggtggtcaa
    12121 caagccgagc ggtttgttgt ctgtgccggg tcgtctggaa gagcacaaag acagcgtgat
    12181 gacgcgcatt cagcgtgatt atccgcaggc agaatcggtg catcgtctgg atatggctac
    12241 cagcggcgtg attgtagtgg cgctgaccaa agccgcggag cgggagttaa aacgccagtt
    12301 ccgcgagcgc gagccgaaaa aacagtatgt ggcccgcgtc tgggggcatc catcccctgc
    12361 ggaaggtctg gtggatctgc cgctgatttg cgactggcca aaccgcccga aacagaaagt
    12421 ctgttacgaa acaggtaaac ctgcgcagac ggagtatgaa gtggtggaat atgcggcgga
    12481 taacacggca agagtggtgt taaaaccgat taccgggcgt tcgcatcagc tgcgtgtgca
    12541 tatgctggcg ctgggtcatc cgattctcgg cgatcgtttt tatgcatcac cagaagcgag
    12601 agcaatggca ccgcgtttgt tgctacatgc agagatgctg acgattaccc atccggcgta
    12661 tggcaatagt atgacattta aagcaccagc ggatttttaa tagtcacgct ggcgaagttc
    12721 gataggccgg agaaggcgtt cacgccgcat ccggcaattt tgcgccgatt gcctgatgcg
    12781 acgcgggccg cgtcttatca ggcctacacg ttgaaactta tttaaaccct ttctgctgct
    12841 ttatcagctc ataggcctgc tgaatttcct gcgctttctg cttcgccatc tccatcatct
    12901 ctggcggcaa acctttcgcc accaacttat cgggatggtg ttcactcatc agcttacggt
    12961 aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca ttacaggcat
    13021 cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat tgctgctgat
    13081 aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca aactgagcgc
    13141 gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc gggtgcagtg
    13201 aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt aagtcaaaac
    13261 gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa ttgtctgatt
    13321 ttcccacccg gaacgcattt tgcgccgcag tacgggaagc gccatgaagg ttcattcggt
    13381 ccatcaactg gctggcgata tgaatatcag cttccgtgac gcgacctttg gatttggtta
    13441 aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca cgctggttgg
    13501 cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt aacaggccca
    13561 atactacgcc ccaaaagccg ccgcccatta ttaaggccac ggtcacgcca atgatttttc
    13621 cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc tcccacaata
    13681 taaaggcgct tttacccatt tctggctgcg gtctattgtg agacatcgcc tataatttgc
    13741 attatcatac ctgtcattca atgccgtgcc taacaccaca gacgctattc ggacaggatt
    13801 aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg cgcaacgtta
    13861 ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct cctggccacc
    13921 atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc ctcacagtgc
    13981 atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa tgacttaccc
    14041 gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt gtttactggc
    14101 agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca gctccatcaa
    14161 aaagaggcac caggacaacc ggagccggtg cgtaccgttg atgcgctcgg taatgtccat
    14221 tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct gaacaccaaa
    14281 gataccaacg tctgggaagg tgattaccag atggtaggtc gccagggtcg cggtaaagcg
    14341 gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg tagctttacc
    14401 tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat tcatgaccgc
    14461 gaagaacaag ttgcggagat ctggaacgcc cgctttaagg tgggtccggt accgatcttt
    14521 tatagcccgt atttgcagtt gccggtgggt gacaaacgtc gctctggttt cttgatcccg
    14581 aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta ctggaacatc
    14641 gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg caacatcatg
    14701 tgggagaacg aattccgcta tctctcccag gcgggcgctg gcttgatgga actggactat
    14761 ctgccttcag ataaagtcta taaagatgaa cacccgaacg atgacagttc acgtcgttgg
    14821 ttgttctact ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa cgtcgactac
    14881 accaaggtca gcgatcctag ctacttcaat gacttcgata acaaatacgg ttccagtacc
    14941 gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt caatgccacc
    15001 gtttcaacca agcagttcca ggtcttcagc gagcagaaca ccagtagcta ctcggcagag
    15061 ccgcagttag acgttaacta ctaccagaat gatgttggtc cgtttgatac gcgtatttac
    15121 ggccaggcag tgcactttgt taacaccaga gatgacatgc cagaggcgac tcgtgttcac
    15181 ctggaaccga ccatcaatct gccgctctct aataactggg gcagcatcaa tactgaagcg
    15241 aagttgctgg caacccatta tcagcaaacc aatcttgact ggtataactc cagaaacacg
    15301 accaggctgg cagagtccgc taaccgtgta atgccgcagt tcaaggtaga cggtagaatg
    15361 gtcttcgagc gtgatatgga aatgctggcg ccgggctata cccagacact ggaaccacgc
    15421 gcgcagtatc tgtatgtgcc atatcgcgat cagagcaaaa tctataacta cgactcgtcg
    15481 ctgctgcaat ctgactactc cggcctgttc cgggaccgga cttacggcgg tcttgaccgt
    15541 attgcctccg ctaaccaggt gacgaccggt gtcacatctc gcatatatga tgatgctgcc
    15601 gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc tcgcactggc
    15661 gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtatg ggcaggcgat
    15721 acttactggc gcatctctga tcgttgggga ttgcgtggcg ggattcagta cgatacacgt
    15781 ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga agaccgtctg
    15841 gtacagctga attaccgtta cgccagcccg gaatatattc aggcaacgct gcctaagtac
    15901 tattccactg ctgagcaata taagaatggt atttcgcagg taggtgccgt cgccagctgg
    15961 ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa tgcgaacaag
    16021 caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcgat tcgcgtcggt
    16081 tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata tgacaacgca
    16141 atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg tacgcaagag
    16201 atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga tttaccacgt
    16261 aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa cgctgcttct
    16321 cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg ataaagtcgc
    16381 agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa tgcagtcggt
    16441 aaaactgaac gctgctcagg caaggcaaca acttcctgat gacgcgacgc tgcgccacca
    16501 aatcatggag cgtttgatca tggatcaaat catcctgcag atggggcaga aaatgggagt
    16561 gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac agaacaacat
    16621 gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca acacctatcg
    16681 taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg tgcgtcgtcg
    16741 catcaccatc ctgccgcagg aagtcgaatc cctggcgcag caagtgggta accaaaacga
    16801 cgccagcact gagctgaacc tgagccacat cctgatccca ctgccggaaa acccgacctc
    16861 tgatcaggtg aacgaagcgg aaagccaggc gcgcgcgatt gtcgatcagg cgcgtaacgg
    16921 cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc tgaacggcgg
    16981 acagatgggc tggggccgta ttcaggagtt gccggggatc ttcgcccagg cattaagcac
    17041 cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc atattctgaa
    17101 agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc atgctcgcca
    17161 tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga aactggaaca
    17221 gattgctgcc gatatcaaga gtggtaaaac gacttttgct gccgcagcga aagagttctc
    17281 tcaggatcca ggctctgcta accagggcgg tgatctcggc tgggctacac cagatatttt
    17341 cgatccggcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga gtgcaccggt
    17401 tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg tcgataaaac
    17461 cgacgcggca cagaaagatc gtgcataccg catgctgatg aaccgtaagt tctcggaaga
    17521 agcagcaagc tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc tgagcaacta
    17581 atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat tggcccggac
    17641 ttaattgtcc agcttgcaca gcgtgagtgg ccggtcgaac aggttgtttg tgccgatgcc
    17701 actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg cccttattcc
    17761 cccaactccc ctgcacaacc gcaaactgcg ggcacattaa cgctacttcc tgtcgcgcta
    17821 cgtgaatctg tcactgtggg gcagttagca gttgaaaatg ggcactatgt ggtggaaacg
    17881 ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat cacaggcccc
    17941 gtgcataaag gcgtcattaa cgacgcaggc attccgttta ccggtcatac cgagtttttc
    18001 gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga acttcgcgtg
    18061 gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac ccctgccctt
    18121 ttgcacgaag tgattgctat tttgcatcac gatttgcgca ccaaatttgg tattgccgaa
    18181 ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca tatgggtacg
    18241 gaagagatag acaccattat tccggtactc aacgagctgc gggaacaggg gatgaaactc
    18301 aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa cgccgacgcc
    18361 gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg cttcgggcgc
    18421 ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca cggcaccgcg
    18481 cttgaactgg cgggacgtgg taaagccgat gtcggcagtt ttattacggc gcttaatctc
    18541 gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc cacttagccc
    18601 gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc attgtttctg
    18661 ccattaaccc gcagaagggc caggcgatgg tcgaaatcgg ccccggtctg gcggcattga
    18721 ccgaaccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac cgcgatctgg
    18781 cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat cagcaggatg
    18841 cgatgacctt taactttggc gaactggccg cgaaaatggg ccagccgctg cgtgttttcg
    18901 gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc tatactgatg
    18961 ccattgccga catgcacttt atgttgcaaa aagaagtggt gaatcgtctg gttgcaggac
    19021 cgaacagcaa ggcgtatggt cgattaagcg tcatggcgca atactattgc aacgtgatcc
    19081 cggtgctgga agtaccgccg tcagccttta caccaccacc caaagtggat tccgccgtcg
    19141 tgcgcctggt tcctcatgca acgatgcctc acccggttaa agatgttcgc gtgttgagcc
    19201 gcatcaccac tgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc ctcggcaacc
    19261 tgtttagcgt cgaggtgtta acgggaatgg ggatcgaccc ggcgatgcga gcggaaaata
    19321 tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg cctttgcagg
    19381 agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg tctacattga
    19441 ggcccaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa ccatacgcaa
    19501 tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca atggcaatgg
    19561 ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta tcgcgcctgg
    19621 cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca ccatgcaggg
    19681 tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc ccgtattccg
    19741 actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta ttggcgacgt
    19801 tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta cccctgggaa
    19861 agataccctc tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc tggatgttct
    19921 gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggtaatc acgatctgca
    19981 tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc tgacaccgct
    20041 gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc ctctgctgca
    20101 aatcgacgaa gagaaaaagc tggtgatggc ccacgccggg atcacgccac agtgggatct
    20161 gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg actcctatcc
    20221 cttctttctt gatgccatgt acggcgatat gccaaataac tggtcaccgg aattgcgggg
    20281 gctgggaaga ctgcggttta tcaccaacgc ctttacccgt atgcgctttt gctttccaaa
    20341 cggtcaactg gatatgtaca gcaaagaatc gccggaagaa gcccctgccc cactgaaacc
    20401 gtggtttgcg attcctggcc ccgtcgctga agagtacaac atcgcctttg gtcactgggc
    20461 atcgctggaa ggcaaaggta cgccggaagg tatttacgcg ctggataccg gctgctgctg
    20521 gggtggtaca ttaacctgcc tgcgctggga agataaacag tattttgtcc agccgtcgaa
    20581 ccggcataaa gatatgggtg agggagaggc ggtagcgtct taaatcccgt cgagcataaa
    20641 atctaatgct cctttaatca acgttcggtg cgcgctggca tcgcaaaatt tcgttcccag
    20701 cgcgttaact ggaacagttg cagtctcatg cgtcatgagc acatattctt tgccgtctac
    20761 cagtaaaagg attgggttaa gccgttctgg tgggcgaata cggctaaatc gttcaatagg
    20821 tgttaatgga ataacgatac ggcgattaat ctcaccaata atgtcgctgg taacatcaat
    20881 aacaaaggga aacgcggcgt tcctgccgcg actgcggtat accgtaaatt gcatcgctag
    20941 aagctccggt actcatcaga gaaacatccg gtttcatcag ccaattgatt taacgcagcg
    21001 atagcttctg cgttctcttc acgccaacgt gttgctgcat gttttttaag ttcagcattg
    21061 agcgcaaccg taagggtggc gctcatattt aagcctgcat cacgagcgcg attgactaac
    21121 tcacggtcga cggtgacggt cacactttgt gtggtacgtt ttgcagtcat gatctcgtgc
    21181 tcttgtacaa tttatacgtc tagtatacac ggatatctgc acgtatcagt gatgccggaa
    21241 ttctaatata caaaattacc gccgctccag aatctcaaag caatagctat gagagttctg
    21301 cgcatcggcg tcgtggaatt cactgaatac cgattcccag tcatccggct cgtaatccgg
    21361 gaaatgggtg tcgccttcca cttccgcgtc gatatgcgtc agatacagtt tttgcgcttt
    21421 cggcaggaac tgctcataaa cgcgaccgcc gccaatcacc atgatttctg gtacgtcacc
    21481 acacgccgca atggcttcat ccaccgactt cacccacgtt acgcgatcgt ccgtacccgg
    21541 ttgactgctg aggataatat ttttgcgtcc tggcaacgga cgaccgatgg attcccaggt
    21601 atggcggccc ataatcacgg gtttatttaa agtgttgcgt ttaaaccagg cgagatcggc
    21661 aggcaggttc cacggcatgg cgttttccat gccgataacg cgatctaccg ctaacgccgc
    21721 aatcagactg atcattgaga tttcccgata aaaaaaattg tcgccactat acgtaaagcg
    21781 taaaccgtcg tcgactggtg cgaggatgat gttgaggaaa attttatatt ctgctggcga
    21841 gtccacgctc tctccctgga ctcgccgcat tacaatgaaa caaaaacaaa ctgttagctg
    21901 taaagcgtga tttacgtcac tctttattag gatgagggct tcgtttccgg ttcatccgcc
    21961 atattgccag tatgtttccc ttcttcggtt ccctgccagc catgtcgttg aattaatgac
    22021 agatgttcac ggtcttcggt aatgatctca cttaacatcg cgctggtgcg tttatagacc
    22081 gccgcacggg ctttggtgtc gttctcaacc attgccatct cttccaccat ctgaatatta
    22141 aagcggcgga acacatcggc acgttctcgc gcttcatacg gccccagacc taaactttcc
    22201 agtgccagac gcccggtttt cagcgcacct tcgaaggttt cacgctccgg cttttcaacg
    22261 cctgcctgac gcaaacgaat gtagtggtcg acatcgcggg cgcgggcaat aatctgcaaa
    22321 tgtgggaaat gttctttcac catctctgtc agttgcaggt tggtttgcgg atcgtcgatg
    22381 gcgttaatca gcacttccgc tttcgccgct ccggcagatt ccagtaaatc catccgcgtg
    22441 gcatcaccat aaaacacttt cataccgaat ttacgcagcg tttcgatatg gtccgggccg
    22501 tgatcgagca ccaccatttt caccccgctg gagagcagta aacgtccggt tatctgccca
    22561 aaacgcccga atccggcaat gattacccgc ggctgttctt cgtcgatctc atcggcttca
    22621 cgcgcctctt cagtagaaga ttgctcaagg cgattgagga tcaccagcag aatcggcgtt
    22681 gccgccatcg acagtgccac cgccagagtc agcgatttcg cccactctgg ctccagcaca
    22741 ttcgccatct gcgccgcacc aaatactaca aaggcaaact cactgccctg tcctaacagc
    22801 accgcaaacc agcgacgctg tttatttggc acctgcaacg gtcgggcaat cagccacagc
    22861 atggcgattt tgatgatgag gaagccgagc agcaaaatga caatgcgcaa tggtttttca
    22921 agcagcgtgc caaagtctat cgacatgcca acgccgatga aaaacagccc cagcagcaaa
    22981 cctttaaatg gttcgatatc gctttccagc gcatgacggt attccgagct tgccagcagt
    23041 acgcccgcca gaaacgcgcc catcgccatc gacaagccga cctcttccag cagcaaacca
    23101 aagccaaaca cgaggaataa cgccacggca ctaaacactt cccgcaagcc agagcgggca
    23161 acaaaacgca gtgccggacg cgtgacatag cgccccagca ataccaccag caccagcgcg
    23221 cccgccactt ttaacgccga gagaacaaat gcgcccatcg tcgtcgaggc actgctcgct
    23281 gccagtagcg ggatcatcgc caccagtggg atcgccgcga tatcctggaa cagcagcacc
    23341 gcaaaggcac tgcggcccat ttgcgtcacc atcagattgc gttcattcat cgcctgcatg
    23401 gcaatcgccg tagaggagag cgccagcgtc atgccgatca attccgcgac ctgccagcgc
    23461 agcccaagta acatgcagaa cagccccagc aggccgccgc aaatcaccat ctgcaatgcg
    23521 ccaccgccga acactgccgc acgcagcttc cacagccttt gtggatcgag ttcgaggccg
    23581 ataataaaca gcatcagcac cacgccaatc tctgcaaagt gcagaataga ttcggcatcg
    23641 gtcaccagtc gcagccccca cgggccaata atgcagccgg cgatcaggta gccaagaacc
    23701 gatcccagac cgagacgtac cgcaatggga acaatcagcg ccgccgaacc gagataaatc
    23761 agcgcctgaa tcagcgtatg gctatccatg atgtgcctcc tgccattcca gcagacgttg
    23821 cttatagtgc cgcgcctgcc cttcgagggt ttcgtcgtca caaataaagg tgcagtgcat
    23881 ggcaaacggt ggcagccagt tcagcccgca gtagattgcc gtcgcctgta gcggctgcga
    23941 cagcacatca aagcccggat gcgcaccaat ctcaaaatgg ctttccccgc cgccggtcgt
    24001 caccgcccac agcaaatgtt tgccgtgcag cgccgtgccg ccgtgaccgt aagcccatcc
    24061 gtgcgagaaa actttatcga tccaaagttt gaggagcgga ggaatgctgt accactgcat
    24121 cgggtgctgc cagacgatca gatcggcacg agacagcgcc tcctgttcgg cggcaatgtc
    24181 gatattgaag tcaggataga gttgataaag agagcgaatt tcgacgcctt ccagcgtcct
    24241 tgcctgttca agcatccgtt tattcgcatg ggaatgatgc ggatatggat gcgcataaat
    24301 tataagaatc ataggtagcc cgcgtgatta cgcctttgtt ttcccacaaa gtgtagtcat
    24361 atattcacgc agcttatagt caataatatt gattcagaaa agagagaaat ctgaacaaat
    24421 taccccattc tctgcccgcg gatcgtcata ttgctggtct gcgccagtga catccctcga
    24481 gtctccgggg caaacgctac ggaaatcaac aagccaaaca gcgagatacc cgcccccatt
    24541 agcatcgtgt tactgatacc gtaattattg ataaagatcg gcagtgccca ggtcgataca
    24601 atagtgccaa tacggcttaa ggacataatc acgcccacgg cagaggcgcg gatatccgtc
    24661 gggaagagtt cattaggata gagccactgc aaattacccg gcccaccaga gaaaaaggca
    24721 tacaccgcaa aggccatcac taccagccag atccccatat ccgggatcag ccccaaaacc
    24781 gccagcgcca gcgtcatcat ggcaaagctg ccaatcaaca atggacgccg tccggcagtg
    24841 tttagccaca gcatcggcgg aatacagccg agcataaaga acagactaat caccacgttc
    24901 cccagcgccg cgtttttgcc aacccccaat cctaacaaac caacgatttg cgggccaaag
    24961 gtgtaaatgg cgaacattgg gatcacctgg caggtccaga tggcggcaac aaacagaaca
    25021 aaaggaaaat ggcggcgatt aaacagatcg cgaaaacggg tttgctgcgg ctgctcttcc
    25081 tcgaaagcca ccggttcgcc aaacagtttt atcatcatct cttcgcactc ttttactcgc
    25141 cctttgcgta acaaccagcg gggagattca ggcagttcga atcgaccaat caaaatcaat
    25201 aaacagggaa tcgctgcgct acccagcatc cagcgccagc cgccttccac atcataaagc
    25261 caatagccga ccaaatcggc gcaggtcgcg ccgacatacc acatcgcggc gataaagctg
    25321 atggaaaacg cccgctgacg ggtactggag aactcggtga tcattgaggt ggcgatggga
    25381 taatccgcac cgataacaat gccgataagt acccgcatca ccaacagttc gacgggggat
    25441 gagacaaaca tcgtcgccac cgatatcacg ccgatggcga tgatatcaat gaggaacatt
    25501 ttgcgccgtc cgactttatc ggagatatag ccaaacagcg atgtgccaac gaacagcccg
    25561 gcgagcgttc ccgcgcccag caagccaatc cagtcagcgt ccagtttcag cgccggagtc
    25621 agttgctcca gcgccacgcc aatcattacc agtacataac catccagaaa cggaccaccg
    25681 cttccccaca gcaaaatgcg gcggtgaata gaggagaatt taagatcatc aaagtttctg
    25741 gacggttgca tgggttattc ctgttttttt gcaatgagag tcagtacggc attacgtatc
    25801 aaaccaacat cagccgtaac ggaactccac accaaaggtg ccgcgcgggt attcccactg
    25861 ttccagcgcc gaccccagcc ccagaatgcg acaggtgccg cactccagac atccggcgta
    25921 atcgaagcgc acactgccgt catcctgctt cttgtacaga cctgcggggc acgctttcac
    25981 cagcagctcc agggcctgtt tatcagcatc ggccttcaca acgatgtgcg gatgctcttc
    26041 atcgacattg aatttattga cgcccagttt gacgtccaca ttgacgggag aagtcataaa
    26101 acggtcactc ctttcatgcc atccttgatc agattgatga agcccacttt cttgccgtgg
    26161 cggaggattt tcttgcgcat cagttccggc gcactgccgt caatggtgaa caggtcacgc
    26221 gccacgccca ccgccagttc cgggtagccg ctaaacatgc gtgggttatc aaggaacgcc
    26281 ggtagtttct ggtacatgcg catatcgcgc agcgggccac tctcaagatg ctgacgatat
    26341 tccgcgagtt tttgcttact gaaatcgtcg cttttcatcg ctgaaagcac agtttttgct
    26401 gctgcttccc cggcggcaat cgccagatcc ataccgcgaa tggtaaaacc gaggttcata
    26461 cacattccgg cagcatcacc ggcaatcaat acgccgtcac caaccaactc cggcagcatg
    26521 ttgatacctg cttccggcac tacgtgagcg gaatattcca ccagcttgcc gcccgcgatc
    26581 agcggtgcaa cggccggatg ttgtttgaaa tcttccagca tttgcggcac cgattttttt
    26641 gcgtcatgca gatgatgcaa accacaaacc agccccagcg acagggtatt ttcattggta
    26701 taaaggaagc caccgcccat caggccatcg gtgggtgatc ccgcaaacag gcaagccgcg
    26761 ccctgattac cctgcaactg aaaacggtct tcgataaccg acttcggtaa ctcgatcagt
    26821 tcctttacgc caaccgccac atccgtcggt ttaacgcgtt ttgccatccc cagcttttcg
    26881 gcaaggatgg aattcacccc atcagcaagg atcaccgttt tcgcttcaat cacatcgcca
    26941 tcggcttcta caccgacgac tttgccatcg cgctgtacga ggttatcgac gcggatcccg
    27001 gtaattaact gcgcgcccgc ttcttcagcc tgctccatca gccaggcatc aaatttactg
    27061 cgcaaaacgg agtaagaacg ctgggatggc gaggtttcgt cgccattgca gtagtccata
    27121 gtcatcgctg acttttccgt cataaacgcg agtttttcat gggtgatcag gcgttctacg
    27181 ggggcggagt cagcgaaacc aggaataatg tgttccagac tgtgggcata gagacgcccg
    27241 ccggtgacgt tcttggcacc tgcggaattg cctcgctcga taactaacac ttgcgcacct
    27301 tcgcgggcga gcactagtgc ggcaaccgaa ccggcaagcc ctgcaccgac gatgatggcg
    27361 tcaaagatat cttcggacat aagagctccc tgtcagcggc agggccaaaa tgcagccctg
    27421 ccagagtgga tcaacgcgct aaagctgcgg tcagtgccgg aaggatcttc acggcgtcgc
    27481 caacaatgcc gtaatccgcg tactggaaga tcggcgcatt tttatctttg ttgatggcga
    27541 aaatggtttg cgacgcatta gcgccaacca tgtgctggat ctgcccggag atccccaccg
    27601 ccaggtacag ttcaggtttc agcatcaggt tggagatacc gacatagcgt tcgtgctcca
    27661 tccatttttc gttttccgcc accggacgag aacaggccaa ctccgcacct atcgccttgc
    27721 aaagctgttc tgccagcgca atgttctctt tgctgccaat accgcgacca acgctgacca
    27781 ccaggcgggc tttgtcgaga tcgacgctgt tgctctgacg cgcctgggtt gccgtgcggg
    27841 taatcgccac agccggagcc tgccactcca cggtgtgcgt ttcgccagtg cgtgacgcgt
    27901 ctggctgagc cacatcgaac gtgccgctgc tgatggtcag aaccgcatac ggcgtggcaa
    27961 tgcgttcttc gccaatcgcc agaccgccgt aaaccatgtg tttcactgtc gctttaccgt
    28021 cctgcacgct aacagcgctg gcatcgttag acaccgccgc gttaaggcgg taacccagtt
    28081 tcgccgccag taatttgccg cgacgggtgt ttggcaacag caccagaccg tctgcgccgt
    28141 gctggcgaat agtgtcagcc atgacaccgg cgtaatcttc gatcatccgc tcgtccggtt
    28201 tgccgcttaa tttccagaca tgattagcgc cgagctggat tgcctgtgcg ccgtcggcat
    28261 cattgaggac gaacgtgttg atttgattag ctaaagcctg cgcaccgttc atcagttccg
    28321 gcagacgaga aggggtatcg ctgaataccc agacttgaga aaacgtgttc atagcatccc
    28381 ctgtaattaa atgactttgc gaagattttc agcaaatgcg gcgatctgtt cttcgccgtc
    28441 gccttcaatc acgatgcgct gacgctcgcg ctgtttcggc gcggcaacct gttgttctga
    28501 ccaggctgcc tctgcgttaa aaccgatatc cgccgccgac catacctgga ctggcttttt
    28561 cgccgcgccg agaatggctt tcatcgaagg aatttgtggg gagttgatat cagtggaaac
    28621 agcaacaacc gcaggcagcg gaatgcttaa ggtttctgtt tcatcttcca gttcgcgctc
    28681 aacggtgagg gtatctgcag tcagggagat aattttgctg acgccgttaa ctgccggaat
    28741 attaaggatt tcgcctacca gcaaaccaac ctgctgggca taaaggtcgg aagaaccatc
    28801 gccacagagg atcaaatcaa agcctgcttt ctgagcggct gcagccagta cgctcgccgt
    28861 ttgttgcggc agtgcctgct cgaactggtc atcaatcacc acaatcagtt catccggacc
    28921 gcgcgatagc acatctttac gccctttggc gttggtcagg gctttaccgc ccacacttaa
    28981 ggccgtcacc tgcgcctctg ctgcctgttg ctttagctgg caagccgctt caatagcgtt
    29041 gagatcgtat tggcttattt tggcatcggc tttgctgaag tctaacgaac catcagcatt
    29101 attgaccgca atatcctgtt catcaggcac gcacttatag caagtaataa tcttcattgc
    29161 atctccagaa atcatgaagg aaacgtcgtt catatcggtg gtgttaatta cagatatcac
    29221 gccctttaat ttggaaataa aactttaatc accaatattg aaaatgtcac acgcagataa
    29281 atccaacttt caatattgtt aagctcctca ccaatattga aaacatggcg tagaaaaaag
    29341 aaattttcaa tattgtttta tggatcacca atattgaaag tcagatgcta tttttttact
    29401 cacaacagaa cataacaaac tgattaataa tcaaaattaa cgaaaaaatg ccctctattc
    29461 acccgaaaca aaaatgtgat aacaatcaca gaatgcagct tattgaatac ccattatgag
    29521 ttagccatta acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa
    29581 acaaacggga acgcaaaata aatgttcgtt ttcacagtgg aattaactca tgaagaatga
    29641 aaagagaaaa acgggaatag aaccgaaggt tttctttccg ccgttaataa tcgtcggcat
    29701 actttgttgg cttacagtca gagatctgga tgcggcgaat gtcgttatta atgctgtatt
    29761 cagttacgtc accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt
    29821 cggctggttc tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccacc
    29881 agaatttagt accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt
    29941 actgttctgg ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga
    30001 accgaactca acaggcgcga aagagttggg gctggcttac agcttgttcc actggggacc
    30061 tctgccgtgg gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg
    30121 caaaatggaa gtgattcgcc ccagctccac cctggtgccg ctggtaggtg aaaaacacgc
    30181 caaagggttg ttcggcacta tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat
    30241 gggtaccagt ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg
    30301 cattccgcat accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc
    30361 catttgcgtc gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta
    30421 cctgagcttc ctgatgctgg gttgggtgtt catcgtcagc ggtgccagct tcatcatgaa
    30481 ctacttcacc gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac
    30541 cgatcccact tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgct
    30601 atccagatga gcatcttcct cgcccgcatc tcccgtggtc gtaccgtgcg tgaactgtgc
    30661 ttcggcatgg tgctggggct gacagcgtca acctggatcc tgtggactgt actcggtagt
    30721 aacactctgc tgttgataga taaaaacatc atcaacattc caaatctgat cgaacagtac
    30781 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    30841 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    30901 accctggcga tgtccacttg ccgcgaagta cgcgatggtg aagaaccccc gctgctggtg
    30961 cgtatcggct ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    31021 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatc
    31081 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    31141 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    31201 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    31261 cgtgacagcg tctacccgga acgttttgtc aaagccctgg cggatatggg tatcgacagt
    31321 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    31381 tggatggaac tgggtcgtct gggagcgcca acttacgtgc tgtatcagtt accgggcggg
    31441 tttaacacct tcctgcgtga aggtacgcaa gagcagatcg acaagattat ggctttccgc
    31501 ggcaccggta agcagatgtg gaactcagca attaccgaac cgggcgcagg ctccgacgtg
    31561 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    31621 tgttttatta ccagcagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    31681 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    31741 gtgaccaaac ttgaaaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    31801 gacgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    31861 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    31921 tgcgcctttg aagatgcggc gcgctacgcc aaccagcgtg tgcagtttgg cgaggctatt
    31981 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    32041 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    32101 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    32161 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    32221 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgc
    32281 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    32341 atcaggccta cgggagcacg aatgtaggcc ggataaagcg tttacgccgc atccggcagt
    32401 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    32461 ggccgttggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    32521 ggcaaatgtt cgcagaatgg ggtgcggaag ttatctggat cgagaacgtc gcctgggccg
    32581 acaccattcg cgttcaaccg aactacccgc aactctcccg ccgcaatttg cacgcgctgt
    32641 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    32701 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    32761 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    32821 gcaccgagga gtacaccaat cttccggcct ataacactat cgcccaggcc tttagtggtt
    32881 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    32941 acttttctgg cctgactgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    33001 ccggcaaagg cgaaagcatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    33061 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgacc aaaggtaaag
    33121 atccctacta cgccggttgc ggtctgtata aatgcgccga cggctacatc gtgatggaac
    33181 tggtgggcat tactcaaatt gcagagtgct ttaaagatat tggcctcgca catctgctcg
    33241 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    33301 cactggtaga agagaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    33361 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    33421 gcaatccaca gtatgtggct cgcgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    33481 cctgcaaagg gccgaatatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    33541 gaatgccctc acatggcatg gacacggctg ctattttgaa aaatatcggc tacagcgaaa
    33601 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    33661 tcagtcagct tgcctgacta gcgcccgctc aataacaaac gaacaaaatg gatagaggtg
    33721 caatggatat cattggcgga caacatctac gtcaaatgtg ggacgatctg gcggacgttt
    33781 acggtcataa aacggcgctg atttgtgaat ccagcagcgg agtcgttaac cggtatagtt
    33841 atcttgagtt aaatcaggag attaaccgca cggcaaacct gttttatgtg ctggggattc
    33901 gcaaaggcga caaggttgct ctacatctcg acaactgccc ggaatttatc ttttgctggt
    33961 tcgggttggc aaaaattggc gcgattatgg tgccgattaa cgcccgcctg ttgcgcgaag
    34021 aaagcacgtg gatcctgcaa aatagtcagg cgtgcctgct ggtgaccagt gcgcaatttt
    34081 atcctatgta tcaacagatt cagcaggaag atgcctctca gttgcggcac atttgcctga
    34141 cagatatggt acttcccgct gatgatggcg tgagttcgtt tactcaacgg aaaaatcaac
    34201 aacctgccac cttgtgctat gcaccgccgc tatcgactga cgatacggcg gaaattctct
    34261 tcacctccgg caccacctcc cgaccgaaag gggtggtgat aactcattac aacctgcgct
    34321 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg
    34381 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
    34441 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtgcaga
    34501 agtaccgcgc caccattacc gaatgtattc cgatgatgat tcgtacgttg atggtgcagc
    34561 cgccttcagc caacgatcgg caacactgcc tgcgggaagt gatgttttat ctcaacctgt
    34621 cggagcagga aaaagacgcg ttttgtgaac gcttcagcgt tcgcttgctg acgtcttatg
    34681 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cggcgctggc
    34741 cgtcgattgg tcgggcgggc ttttgctacg aagcggagat ccgcgacgat cacaatcgtc
    34801 ccctcccggc tggtgagctc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
    34861 tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gatggctggc
    34921 tacataccgg cgataccgga taccgcgacg aagagggctt tttttatttc gtcgatcgcc
    34981 gctgcaatat gatcaaacgc ggtggtgaga atgtctcctg cgtggagctc gaaaatatca
    35041 tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg
    35101 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
    35161 tcttctgctt ctgtgaacag aatatggcga aatttaaagt gccctcttat ctggagatca
    35221 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
    35281 aacggcaggg aactaccctg tcagctaata atcacgctct aaaacaatat gcattttttg
    35341 cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
    35401 atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa gcgaatgcta
    35461 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
    35521 aattacgtgt cgccattatt accggtgccg gagagaaatt cttttccgcg ggctgggatt
    35581 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccggga ggttttgcgg
    35641 gattaaccga aattttcaat ctcgacaaac cggtcatcgc cgctgtgaac ggctatgcct
    35701 ttggcggcgg ctttgaactg gcgctggcgg cagattttat tgtttgtgcc gataacgcca
    35761 gcttcgccct accggaagcc aaactgggca tcgttcctga cagcggcggc gtgctgcgtc
    35821 tgccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg
    35881 gcgcagaaga ggcgctgcgt tggggggtag tcaaccgcgt ggttagccag gcggaactga
    35941 tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgctccgctg gcgattgcgg
    36001 cgctgaaaga gatctaccgc accaccagtg aaatgccggt agaagaagcg tatcgctata
    36061 ttcgcagcgg cgtgttgaaa cactatccat cggttctgca ttcggaggat gccattgaag
    36121 ggccgctggc gtttgccgag aagcgcgatc cagtgtggaa aggacgttaa caaccgtgag
    36181 ctattacgcc tttgaggggt taattccggt tgttcacccg acggcgtttg tccatcccag
    36241 tgcagtattg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
    36301 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
    36361 cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggtc atatcgggca
    36421 cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ctggtcggta tgaacagcgt
    36481 gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgctatga gctttgtcaa
    36541 agcgggcttt cgcggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtgcg
    36601 cagtgtgagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
    36661 tgtcgggcgc tgtcatgcat cgttacatga aacgcagcca ctgaggcaaa tggaggaaaa
    36721 tcgcccccgt ttgcagggga cgacggatgt gacgccgaaa cggtgatctt tccggatgcg
    36781 gcgtaaacgc cttatccgac ctggctggaa agcaaccgta ggccggataa gatgcgccag
    36841 catcgcatcc agcgaccagg tcgcacggta tcaacgaaat gacattaacg acgcatactc
    36901 tttgacaaca tcatctgcca cttctgctcg cggttaagtt ccgtgagcgg aacggcagga
    36961 atacgcggcg tctttcttac gccgactaat ttatcccggc tgttattgcg caggcgcgcg
    37021 taaatttgct catcgatatc aaccactttt accagtcgct ggcactggca tccccgccct
    37081 tccagcttat tagggatcat cttaacttcg cagcttattt ctgtgacttc cgacagaata
    37141 taagtcaggg tattaaccgc cttgctgtgt tcaatatcga aatgaataga gatctctctt
    37201 gctatcaccc accggttttc agccatcatc cattcggcga ttaacaaata gagtggtttt
    37261 tcaacacatc cttcacacat acgaactcca gtttcacaaa ttaaaagact ggctatgact
    37321 cgttttgcag caattaaaca aggccatatt aaaattgtgg atatatcgat agtatgcaac
    37381 ttcaccgtcg ataagtgtga gctaaatcct gcttatgtca tcaaaaatat cgattcccca
    37441 aaagatttac ttaatgggca aaaaaaacgg tcctcatcag agaaccgtat tagttattcg
    37501 atcaaattag cggatgaaaa atatacgcca tgacacgcta ttatttgatc tgcgcgtgca
    37561 tttcctgcac cgaaattact ttttcggtcg catcggcatt cagcgccatc gcggtggcaa
    37621 agccgccgtt cagggtggtg tcgtaatgca ctttatattg cagcgcactg cgacggatca
    37681 cgcgggagtc ttcaatcgca cgacgacctg aggtggtgtt gatgatgtag gtatattcgc
    37741 cattcttaat acggtcctga atgtgcggac ggccttcatg caccttgttc accagacgcg
    37801 gattgatacc cgcttcgccc agcacaatcg ccgtgccgtg agtcgcatcc agctcgaagc
    37861 cctgtttcag cagttttgcc gccaggtcca ccacgcgttc tttatcgcct tcgcgcacgg
    37921 aaagcagcgc acgaccgtgt ttcttcatgg tggagttgct gcccagctgc gctttggcaa
    37981 acgcttcagc gaaggtgcgg cccacgccca ttacttcccc ggtagagcgc atttctggcc
    38041 ctaacagcgg gtcaacgccc gggaatttat tgaacggcag caccacttct ttcaccgagt
    38101 agtacggcgg gataacttct ttggttacgc cctgctcagc cagcgatttg ccagccatca
    38161 cacgcgccgc cacttttgcc agcggtacgc cggtggcttt ggagacgaac ggaacggtac
    38221 gcgccgcacg cgggttaact tcaatcaggt agacttcgtt gtttttcacc gcaaactgca
    38281 cgttcatcag gccgcgcacc tgcagttcga aggccagttt ctgcacctgc tggcgcatca
    38341 catcctgaat ttcctgactt aaggtgtaag ctggcagtga acatgcggag tcaccggagt
    38401 gcacacccgc ctgctcaata tgctccatga tgccgccaat cagcaccatt tcgccgtcgc
    38461 agatggcatc cacgtcaact tctactgcgt catcaaggaa gtggtccagc aacactggcg
    38521 catcgttaga tacgctgacc gctgtctgga agtagcgacg caggtcagct tcgtcataga
    38581 cgatttccat cgcccgaccg ccgagaacgt aagacggacg caccaccagc gggtagccaa
    38641 tctctttcgc tttctcaacc gccatttcga tggtggtaac ggtggcgttc gccggttgtt
    38701 tcagtttcag acgctcaacc gcatgctgga agcgttcacg gtcttccgca cggtcgatgg
    38761 catccgggct ggtaccgata accggtacgc cagcagcttc cagcgcgcgc gccagtttca
    38821 gcggggtctg accgccgtac tggacgataa cgcctttcgg cttctcaata cgcacaattt
    38881 ccagcacgtc ttccagagtt accggctcga agtagaggcg gtcggaggtg tcgtagtcgg
    38941 tggagacggt ttccgggtta cagttaacca taatggtttc ataaccatct tcgcgcagcg
    39001 ccagcgaggc gtgtacacag cagtagtcga attcgatacc ctgaccgata cggttcgggc
    39061 cgccgccgag caccatgatt ttttcacggt cggtagacgg attcgcttcg cactcttctt
    39121 cataagtgga gtacatgtaa gcggtgtcgg tggcgaactc tgccgcacag gtatccacgc
    39181 gcttataaac cgggtgcagg tcatactggt cgcgcagctt gcggatttct gcttcacgca
    39241 cgcccgcgag ttttgccaga cgcgcatcgg caaagccttt gcgtttcagc tggcgcagga
    39301 attcagcgtg caggccagtg atgcccactt ctgcgacttt ctcttccaga cgcaccagct
    39361 cttcaatctg taccaggaac cagcggtcaa tgttggtcaa attaaataca ccgtctacag
    39421 acaggcccgc gcggaaggca tcagcgatat accagatacg ctctgcgcca gcatctttca
    39481 gttcgcggcg gattttagtc agcgcttccg ggtcgtcgag actcactttc ggatcgaagc
    39541 cagttgcgcc cacttccagg ccgcgcagcg ctttttgtag ggattcctgc tgcgtgcgac
    39601 caatcgccat cacttcgcca accgatttca tctgagtggt cagacggtcg ttagcaccgg
    39661 cgaatttctc gaagttgaag cgaggaattt tagtaaccac atagtcgatg gacggctcga
    39721 aggaggccgg agtacgccca ccggtgatgt cgttcatcag ctcgtcgagg gtgtaaccca
    39781 ccgccagttt cgccgccact ttggcaatcg ggaaaccagt ggctttcgac gccagcgcag
    39841 aggagcgaga cacgcgtggg ttcatttcga taacaatcag gcgaccgttt ttcgggttca
    39901 ccgcaaactg gacgttggaa ccaccggttt caacgccgat ttcacgcagt accgccatcg
    39961 aggcgttacg catgatttga tattctttgt cggtcagcgt ttgggctggc gcgacagtga
    40021 tggagtcacc ggtgtggatg cccatcgcat caaagttttc gatagagcag acgatgatgc
    40081 agttgtcgtt tttatcacgc accacttcca tctcgtactc tttccagccg atcagcgact
    40141 catcaatcag cagctcttta gtcggagaaa gatccagacc gcgggcgcaa atttcttcaa
    40201 attcttcacg gttataagcg ataccgccac cgctaccgcc catggtaaag gatgggcgaa
    40261 taatgcacgg gaagccaact tcagcggcaa ccgccagcgc ttcttccatt gaatgagcga
    40321 taccggaacg cgcagtttcc agaccgattt tcttcatcgc tacgtcgaaa cggcggcggt
    40381 cttcggcttt atcaatcgca tcggcagtgg caccaatcat ggtgacgccg aactcttcca
    40441 gcacgccctg acgctcaagc tccagcgcac agttcagcgc cgtctgcccc cccatggttg
    40501 gcagcaccgc gtccgggcgc tctttttcaa taatcttgcg taccacttcc cagtgaatcg
    40561 gctcgatgta ggttgcatca gccatttccg ggtcggtcat gatagtcgcc gggttggagt
    40621 tcacgagaat gacgcggtaa ccctcttcac gcagggcttt acacgcttgc gcgccagagt
    40681 agtcaaactc acacgcctga ccgataacaa tcgggcccgc acccagaatc aggatacttt
    40741 ttatatctgt acgttttggc atggctcttt tactcctgat tacttagcgg ttttacggta
    40801 ctgctcaatt aacgcgataa agtggtcaaa caacggcgcg gcgtcgtgtg gaccagggct
    40861 ggcttccggg tgtccctgga agctgaacgc cggtttatcg gtacgatgaa tgccctgtaa
    40921 cgtaccgtcg aacaaggatt tatgtgtgac acgcaggttt gcaggtaatg ttgcttcatc
    40981 caccgcaaaa ccgtggttct gggcggtgat catcaccacg tttttctcaa catctttaac
    41041 cggatggttg ccgccgtggt gaccaaattt cattttgaca gtcttcgcac cgctcgccag
    41101 cgccagcagc tgatgaccga ggcagatgcc gaataccgga atatcagttt cgaggaattt
    41161 ctggatagcg gtaatggcgt aatcgcacgg ggccgggtcg ccaggaccgt tggagaggaa
    41221 gatgccgtcc ggattcattt tcagcacatc ttccgcagaa gtttgcgccg gaacgatggt
    41281 caggcgacag cctctatcca ccagcatacg caggatgttg cgcttggcac cgaaatcgta
    41341 ggccacgacg tggtacggca gctcgtcttc ttttttcgct tctggcaggc cgccggtcag
    41401 tgtccagctc ccttgtgtcc agctataggg ttctgcggtg gttacttctt ttgccagatc
    41461 catgccgttc agacctggga acgcgcgggc tttttctaat gccagcgccg catccgggtt
    41521 atcgcctgcg ataatgcagc cattctgagc acctttctca cgcagtaaac gcgtcagctt
    41581 acgggtatcg atatcggcaa tcgccacgat gttatggcgc ttcaggtaag aagagaggtc
    41641 ttcggtatta cggaagttgc tggcaatcag cggcaggtcg cgaatcacca gaccttgggc
    41701 atgaacctga gaagattctt catcggcgtc attagtgccg acattgccaa tatggggata
    41761 agtaagagta acgatttgac gagaatagga aggatcagtg aggatttctt gataaccggt
    41821 cattgaagta ttgaaaacga cttccccaac cgccgaacct gttgccccta tggcccgacc
    41881 gtgaaactgg gttccgtctt ccagaaccaa tagcgctgac ttaatcaaaa caccctccag
    41941 agaatattca ctcaccttat ttgcatatta attcacaata atgacgtaaa tcaatgcaaa
    42001 tctgcttacc gacgaatttc tggcaaacgg cggcattctg gagatatggg agctaaaagt
    42061 caacttaatg gcgggatttt ttatattatt ttgtggctca tgcatgtttt acaggcatta
    42121 agcgacaaaa agatcatata acatgttctg gaaaacgctt gcgcagcaag aagattgcat
    42181 gaaatcaaaa tgagcagaaa aaagtggacc aaatggtcaa aatttacatt aaaacaacaa
    42241 aaacagaata caaaacatgt aattttcagc atttgcatta gatttaaaag cataacacta
    42301 aaaataaaag ggccaaaaat taaagccctt tatattcaac gaattaaatg tgttgttttt
    42361 gcatcaccat aaatatcttg tggttacaga ttattgagat caagcacatc tcgcatatca
    42421 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcacc gttagcaaat
    42481 gtcatgcggc tggatgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
    42541 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
    42601 acacgttcac cggtatggcc ttcacgactg tagaccgcgc aatctttcag atctttatca
    42661 agagcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
    42721 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cccccatcac tttggctgct
    42781 ttctccagca gtttaagcat gacgttaaca ccaacgctaa aattggcggc aaagacaatc
    42841 gcaatatcgg cggcggcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
    42901 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
    42961 ggacgggtaa aatcgataaa cacatcaaaa tcatctttta tcgcatcaag actactttgc
    43021 acagtaacgc ctgttttccc ggctccggcc agttcgcccg catcgctgcc cagtaaagaa
    43081 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
    43141 atcaactggc ggcccatacg gcccccggct cccgcgatgg caacgcggat gtttgcatca
    43201 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
    43261 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
    43321 agtgaaaggc atgacgcaga aagtgatgcg tcataccctc tgtcattatg cctggtcttg
    43381 tgactttgac ggcaggcagc acagtataag cagaattacg cccgtaacag ggatcaacac
    43441 gacaatggta acccaccacc ctgaatatcc gatatcgtgc atccttctga cggctaacgc
    43501 tatcgccggt acggcaaaaa tcaacgttaa aataaatgac gcgggcagcg caaaaaaaat
    43561 gaaaacacca tagtcattgt taattgttac cgctaaccac caacataaaa gcggaagcag
    43621 acagataacg ccactaccaa tcataaacga ccagaactcg aagcgattgg aaacaccttc
    43681 ataattaaag atgtttttcc acccttccag atacgcttct ccatacgtca tagaacactt
    43741 ccttgtttta ttattagagc gccagggcca gcacctcagc cacccattgc tggaagcctt
    43801 tcacatccag atccagcgcc acctgtacat tggctggttt gcccaagcaa ccgtcgatat
    43861 caacaaccgt cgtgcctgag gtaaattcac cctgagtttc taccgcgaca aaacacggtt
    43921 tgagagtgaa caattccggg cgcaccagcc aggcgatagc gcaaagatcg tgcatacgta
    43981 gtccactttg catactgccg ctacggtagt ggctaaacag ggcgtgtagc atttttccgg
    44041 tgcgattcaa ttccgacaaa gtagcgagat agtcaggggt taatattgcc tgattggtga
    44101 catccagacc gcacatgacg atttcgatgc cgctacggaa gacacaggct gccgcttctg
    44161 gatcggcagc gatattaaac tcggcgtttg gtgtgcagtt gccgcgacca gcagaaccgc
    44221 ccataatcac cagacggcaa atatacggct tacattccgg gcattgtgaa agtaacagcg
    44281 caatattggt taacgggccg atggctacca gggtaacggg ctctggcgca cgcatcaggg
    44341 catcacgaat tgccagaaac gccggtatac ccagtggctg acggttgtgt tcaacaaagt
    44401 catagccagc cattcccgat tcaccgtgta cagatgccgc atcgcgcggc gcacgaacca
    44461 gtggtacggc agcgccctgg gcaagcggaa tatccacatt ccagaaatgc agcagttgca
    44521 gggcgttgcg ggtcgttttc tctaccgaga cattacccgc gacagtggtc atcagttgca
    44581 gatcgagttc gggggcaaaa atcgcggcgg caatggcaac ggcatcgtca atgccggggt
    44641 ctgtatcgag gaagataggt aaacgcatgt tttctccata aaaaatgccg gtaacaagac
    44701 cggcattttc gcataactta ggctgctaat gacttaatca acttcacgaa tatcgacacg
    44761 cagctctttc ggcacttcga aaacaatgtt ttcttcacga ccttccagcg gaatggcttc
    44821 gccaccgcca agctgctgta aacgtgcgac tacgttctgt accagaatat ccggagctga
    44881 tgcgccagca gtcacgccga cgcatttcgc ctctttcacc cactcttcct ggatatctgt
    44941 cgcatcgtca atcaaaaacg cgcgtttgcc catacgctgg gccagttccg ccagacggtt
    45001 ggagttggaa gagtttttcg aaccaaccac cagtactact tccgcctgct ctgccagggc
    45061 gcgtactgct tcctgacggt tagtcgtggc gtagcagatg tcatctttgc gcggaccgac
    45121 aattttcggg aagcgtttac gcagcgcgtc gatcacatca gacgtgtcat ccaccgacag
    45181 cgtggtctgg gtcataaagg agagcttctc ttcgtttttg accgtcagtt tccacacatc
    45241 gtctggcgat tcgaccagat acattccccc ttccgggttg ctgtactggc ccatcgtccc
    45301 ttccacttcc gggtgcccgg cgtgaccgat gagaatagat tcttcaccac gacggctggc
    45361 gcgggcgact tccatatgca ctttggtcac cagcggacag gtggcatcaa acaccgtcaa
    45421 atcgcggctt ttcgcttcgt tacgtactgc ctgagaaaca ccgtgtgcgg agaaaatcag
    45481 gatcgcgccg tccggtactt cgctaatctg ctcaataaag atagccccac gctcgcgcaa
    45541 gctatcgacc acgtagcggt tatgcaccac ttcgtgacgg acatatatcg gtgcgccgta
    45601 aatggccagc gcgttttcaa caatgctgat agcgcggtct accccggcac aaaaaccacg
    45661 cgggttggcc aacaggatct gcatgttacg cctccagtgc cggatcgatt tccagcactt
    45721 caatatcaaa atgaacggtc tgcccggcca gcggatggtt gaaatcaacg gtaatggagt
    45781 cgccgttaat ttcgcggatc acgccaggca tctcactgcc atccattgcg gtaaaaagca
    45841 tgattgcgcc aatttctggc tcgcctgcat ccataaattc acggcgggag aagtactgaa
    45901 tcaggtccgg tgacggcacg ccaaacgccg catctggctc cagcgagaag gtggttttat
    45961 cgcccacttt cagccccagc aggtgttgct ccagcccttc agaaagagaa gcatcaccca
    46021 ggcggaacag cgccggttta ccgttgttgc gggtagactc agcggtggtg ccatcgtcga
    46081 gttttagcgt gaagtgcacc aggacggcgc tattgctctg tacagattca gacatgcagg
    46141 tttgctctta tattttgagg atttttgccg gatgcgacct acgatttcgc agcaatctgt
    46201 aggccggata agatgcgtca gcatcgcatc cggcagggtt tattattgtt ttttcgcttt
    46261 agaaggtaaa aaaccttcca gcacaatcag tgccgcaccg acacaaatgg cagtatcggc
    46321 aaggttgaag gtggcgaagt gccagtcgcc gacatagaag tcgatcatat cgacaacgaa
    46381 gccgtgccac aggcggtcga acaggttgcc cagtgcgccg ccaataatca gcgcgtaagc
    46441 gatattgttt agcttctgcg tggccttcga gcgatacatc atcactgcca ggatcacgct
    46501 aataccaatc gcaataccgg caaagaacca acgctgccag ccgccgctat cggcaaggaa
    46561 actaaacgct gcgccatagt tacgcgcata atgcagatta agcgacggga acagcgggac
    46621 cgtatccccc agagcaaagt tctggaggat caggtatttg ctgcccagat cgataatcag
    46681 cacgactacc accagccaca gccagcgtag ccctgttgaa cagatcgatt gactcatcag
    46741 gcaaacttac gtttttcacc gtcaccggcg acgttgctga cacagcggcc gcagatttct
    46801 gcgtgttccg ccaccttgcc gacatcctgg gtgtagtgcc agcagcgagg gcacttctca
    46861 ccttcggctt tactcaacgc gactttcagc cctttgagga cttcgctctg ctgggcgtca
    46921 gcaggtgcgt cgttatagtc tgcaacggta gcgccggagg tcaacaggac aaatcgtaat
    46981 tcatcgccca gcgcggtcag tttcgccgcc agttccggtt ctgcatacaa ggttactgcc
    47041 gcttccagcg agccgcccac tttcttatcg gcacgcgctt gctcaatgac tttgttcact
    47101 tcgccacgca ctttcaacag ctcgtcccag aacgcatcgt tcattgcttc actgtctgcc
    47161 agaccaaaca ggccttcgta ccactcaccg gtgaagacgt atttttcacg ttcgcccggc
    47221 aggtagcccc acacttcatc agcggtgaag gagaggattg gtgccatcca gcgaaccagc
    47281 gcttccgcga tgtgatacag cgcagtctgg cagctacgac gcgccacact gtccgctttg
    47341 gcggtgtact gacggtcttt gatgatgtcg aggtagaagg aacccatctc aacggagcag
    47401 aagcgcatca gacgctgtac cacttcgtgg aaatcgtatg cttcgtacgc cttgaggatg
    47461 tcttcctgtg ccgctttcgc acaacctacg gcccagcgat ccagtaccac catctcttcc
    47521 ggtttcacca tatcttttgc cggatcaaaa ccgttcaggt ttgccagcag gaagcgcgcg
    47581 gtgttacgga tacgacgata gctgtcggca gcacgtttca ggatctcgtc agaaacggcc
    47641 atttcaccgg tgtagtcagt tgatgccacc cacagacgca gaatatccgc gcccagtttg
    47701 ttcatcacat cctgcggcga aacggtgttg ccgatggatt tagacatctt gcggccctga
    47761 ccatccacgg taaagccgtg agtcagtacc tgacgatatg gcgctttgcc cttcatcgcg
    47821 gtagagatca tcagggaaga catgaaccag ccacggtgtt ggtcagaacc ttccagatac
    47881 atgtccgctg cgtgaccggc aaattccgga cgcacgtcaa caacggaaga gtgggtagat
    47941 ccggagtcaa accatacatc cagcgtatcc ggtactttca catactggtc agcttcgtcg
    48001 ccgaggatct ctttcgcatc gagatcccac cacgcctgaa tgccgtcaac ttcaacgcgt
    48061 tttgccactt cttccatcag ttcgagagta cgcggatgca gttcttctgt gtctttgtgc
    48121 acgaacagtg acatcggcac gccccaggta cgctgacgag agatacacca gtcaggacgg
    48181 ttagcaacca tcgactcgat acgcgcctgg ccccagtcag ggatccactg cacgcctttg
    48241 atctctttca gtgactgcgc acgcagacct ttctgatcca tgctgacgaa ccactgcggc
    48301 gtcgcgcgga agatgatcgg cgttttatga cgccagcagc acggatagct gtgttgcatt
    48361 ttctcaacgt gcaacagtgc gcctttttcc tgcaacaacg caatgacaat atcgttcgct
    48421 ttgaagacgt taacgccatc cagagtcggg taagtacccg gcagataagt gccgtccggg
    48481 ccaaccgggt tagcggtttc cagaccatat ttttgaccga tcacatagtc gtccggaccg
    48541 tggcctggcg cggtatgaac ggcaccggta ccggcatcca gcgtaacgtg gtcgccgaga
    48601 attgccggaa catcgaagtc cataaacgga tgggtaaagc gcaacagttc cagctcggca
    48661 cctttcaccg tgccaagaat ggtgtaatcg ctaacgccga tacgctgcat tacgctttca
    48721 accagatctt tcgcgaggat cacggcctga ccgtcgattt gcaccagcgc ataatcaaaa
    48781 tcaggtgcaa tggagattgc gcggttagca ggcagcgtcc acggcgtggt ggtccagata
    48841 accagcgaaa ttgggccgtt aacattgctt acgccaaatt tcgttttcag cgcatcctga
    48901 tcgaccgcct ggaaagcgac gtcgatggac ggagaagttt tgtcgtaata ctcaacttcc
    48961 gcttctgcca gtgcagaacg gcagtcaacg caccagtgca ccggcttcgc gcctttgtgc
    49021 aggtgaccgt tgccgatgat tttgcccagc gcgcggatga tgttggcttc agttttgaag
    49081 tccatggtca ggtacgggtg cgaccagtcg cccagcacgc ccagacggat aaagtctttg
    49141 cgctgaccgt caacctgggt cgcagcgtat tcgcggcact tggcgcggaa ctcagcggcg
    49201 gtgaatttct cccccggctt accgtattct tgctctactt tcagttcgat tggcagacca
    49261 tgacagtccc agccaggcac atacggcgag tcatatccag aaagcccttt ggacttaatg
    49321 ataatgtctt tcagaatctt gttaaccgag tgaccaatat gaatgctgcc attcgcataa
    49381 ggagggccat catgcagaat gaaggttttt ttgccttttt tagccgcacg gatgatgccg
    49441 tacagatcat catcagtcca acgcgccagc attcccggtt cgcgcttggc gagatcgcca
    49501 cgcatcggga accctgtttc cggcaaattc agggttgatt tatagtcact catcagattc
    49561 tcggttccgt atttcggttt gattacataa caggcttaag ccggttttgt tagcccaaaa
    49621 aattcgcggg cggttaattc atcacgcgca atctgcgctt tcagttcgtc cagcgatgca
    49681 aatcgctgct cattgcgtat ttttttacgc agcactactt gtatatggcg accataaagg
    49741 tccattgcaa catctaacaa atgcacttcc agttgctggc gaataccggc aaccgttggg
    49801 cgtgttccga tgtttgcaac gccgggtaac ggcttttcgc caaggcccaa cacttctacc
    49861 gcataaaccc ctttcaccgg ggaaacctga cggcgtagcg gtacattcgc cgtcgggaaa
    49921 cctatagtgc gccctaattc atcaccgtgg actacacgcc cggagatagc aaacgggtgc
    49981 cccagtaaac tttctgccag agccagattg tcatccgcaa gcgcctgacg cacggcggtg
    50041 ctgctgatac gcacaccacc ttcgcaaaaa gtttgcgtgc tggtgatatc gaagccgtat
    50101 tccatgcccg ctttctgtaa taacaagaaa tcgccttcac gaccagcgcc aaagcggaaa
    50161 tcgtcaccta cggcaagaaa ttttacccgc aagtgcttca ccagaagatc actgatgaag
    50221 ttttgcgcgg ttaacgccgc aaaacgcctg tcaaaacgca cgcacagcac gtaatcaacg
    50281 ccacactctg caagataacg cagtttttcc cgcagccggg tgagccgtgc cggggcttta
    50341 tcagtagcaa acagttccag tggttgaggt tcaaaaagca tcaccatcac cggtaagttg
    50401 cgcttgcgcc cttcttcctg caagccctgt aacagcgcgc gatgaccgcg atgcacgccg
    50461 tcgaaattac caatagtcag cacacaccct tcttgcgggg cctggctgag attatgtatg
    50521 ccgcgtatca gcttcatgtc tggctcaaaa cagtgaaaat cgtccgagta taccttgtac
    50581 agcggtcaag gttaaccggc gattgagtac cgaaatcaga aagaagtggc ttttcattgc
    50641 tatggcgcaa atcatgggaa gaaactgacc gcctgctgca atttttctcg cggaaaagct
    50701 gtattcacac cccgcaagct ggtagaatcc tgcgccatca ctacgtaacg agtgccggca
    50761 cattaacggc gcttatttgc acaaatccat tgacaaaaga aggctaaaag ggcatattcc
    50821 tcggcctttg aattgtccat atagaacaca tttgggagtt ggaccttggc taatatcaaa
    50881 tcagctaaga agcgcgccat tcagtctgaa aaggctcgta agcacaacgc aagccgtcgc
    50941 tctatgatgc gtactttcat caagaaagta tacgcagcta tcgaagctgg cgacaaagct
    51001 gctgcacaga aagcatttaa cgaaatgcaa ccaatcgtgg accgtcaggc tgctaaaggt
    51061 ctgatccaca aaaacaaagc tgcacgtcat aaggctaacc tgactgcaca gatcaacaaa
    51121 ctggcttaat tgcctgttgt tgtcagcttt gtaaaaaaac ccgctctggc gggttttttt
    51181 atatccatct gcaggccaaa attcggcctg caaagtacaa atgtttattt tgaagctgga
    51241 gtaaacagcg cagaatagtc tgtattgcag atacgctgta ctgcagggtg ctgaatcatc
    51301 ctttcggcaa aaatcgcgtg gtactcttcc atcacgttct caacacggcc tatctccaca
    51361 accgagtcat cgttatagaa atcattagcg taaagcgaag gtgcaacgaa aatagcgtta
    51421 tgcgtcgccc caaaggcttt catcaacgca gcatcatcaa actcacccaa aatttcgacg
    51481 ttcaagccct gggagttaaa ccagtttaat agtttacgcc ccaacattga gcgacgcccc
    51541 ggaataagta aacgacgctc ttcaagacag gcaggaaacg gcttttctgg tagtgggtta
    51601 gtgcaccaga aactgacacc acattcgcca attttcatgg aaaacagccc ttcctgctga
    51661 gtggaatcga tcggacagtc agagatgatc atatccagtt tatgctgact caactgctct
    51721 aaaagcatct cgtgcgtcga ttcgaaacag cgtagatgga tctgctcgtc ttccacaact
    51781 gcggcatcca gaacactgct gaccagacgt ttggaaagtg catctgccac accaacatca
    51841 aacaataagt tggactcttt gcgatagttg acgatatcca gcatttcctg gcttaaggtg
    51901 aacattttat cggcatagcg atagaccagt tcccccagtt cgctgggttc cagaccacgt
    51961 cctttacgct taaatagttt cccttgcagg cgctcttcca gcgcccggat ctgcccggta
    52021 atggtttgtg gtgttaaata aagcgcctcc gctgcgccaa ccacagaacc ttctttgtag
    52081 acatgccaga agtaatacaa gtggttgtaa ttgatatgag acatgctcat ttctctccct
    52141 gataacaatg aaaagggagc cgtttatggc tccccagtac atcgtcctgt caaactgatg
    52201 gacgtaaacg aacgcgtaac cagctatatc caattaccgc cgaagaaatt gaaccgacaa
    52261 ggatacctaa ttttgcccag ttaatcagtt ctggatctac gctaccaaat gccaggctgg
    52321 caataaagat agacatagta aaaccgatac cgcacaggat accaaccgcc ataatttgct
    52381 ggtaagtcgt tccctctggc agatgtgcca atttcaaacg cagcgccaac cagcagaaca
    52441 gactaatacc cagtggcttg ccaatcagca aaccagcgat gatccctaat ggcagaatgg
    52501 aggtcaaacc ttccagcgtg acaccttgca gtgaaacgcc agcattagca aatgcaaaca
    52561 gcggcaaaat cagatacgcc acccatggat gcaaaacatg ctccagacgt ttagccggag
    52621 agcgcccatg cttctctttc aaaggaataa agaagccgac aatgacgcca gccagggttg
    52681 cgtgaacccc cgatttcaac accgctgtcc acagcaccac gccaaccaga atataaacgc
    52741 ccgtgcggcg tacaccacac agattcaata ccgcgagtac cgcaattgct acagccgcga
    52801 cgccaagaga ggccatcgat aagtcattag tgtagaacaa tgcgatgata atgatggccc
    52861 caagatcgtc gataatagcc agagccatca aaaagatctt cagcgctaac ggaacacgac
    52921 ttcccaacag cgccaacaca ccaagtgcaa aggcaatgtc agtcgccgcc gggattgccc
    52981 agccttcgcg ggtaatcgga tcggcatagt taaaagccag atagagcaat gccgggacaa
    53041 tcatcccgcc gattgcggca ataacaggaa atgccgcctg gcgcagactg gccagcgaac
    53101 cttgcatcag ctcgcgttta acttccagac caaccaacag gaaaaatacc gccatcagag
    53161 cgtcattgat ccatagcagc atgttcttgt tgatctcaag tgtcccaacc cggagctgaa
    53221 ccggcgtctc aagaaagtcg tgataccatc cactggttgc accgctgttg gccataatca
    53281 tcgctaatac agcggcaata atgagaataa tgcctcccga ggcatcactg ctaaagaatc
    53341 gatgcagatg tttcactttt tatttctctt caggtgaata gatcgttttc acgattttac
    53401 cccgcacgaa tcatcgttaa aatcagatta tagtgtagga atagctcgct ttttacgatc
    53461 tattaaaaga attgcgccat cagcgagaga tgagtttttg atgcccgaaa gcggcggctg
    53521 atgaccttaa accccattaa tcatagtcta atgtcaagat ttctgaagaa attattcgga
    53581 aaagtaataa ttaaattgtt ccatgctcta gttttttatt ccacagatgt ttgaggatat
    53641 ctaatgtaga aacgacattc gcaggagaca ttcggttcaa tgatataagg aaaggatggt
    53701 tctccacaaa gtggataaaa cgcgtcgcac tgttcaatgt tgcatgccgc atctcgcgga
    53761 ctttcttact gccagtgaga ttgcatacag ccattgaata attgcggtta gaaaatgcac
    53821 gccagacatt atttcgtacc ccaccactaa ggaataatcg cggtggaaaa tcaatggtgg
    53881 cgggtgccac accgcgctct ttgacaaagg aaatatggca gcggccttga tgaatatgct
    53941 gcaaattagc catcaggcag ccgcccaata taaagcattc gtgatcaagg taatgctgaa
    54001 cgttgttgga atgctcatcg gtcagtattc tgaccagttc aaaatgacga tatgccggaa
    54061 gaaccggttt taccaacatt cccggatcgt tttgatgtaa aactgccggg ccgtaatcaa
    54121 tatcaaacaa ggtatcccgc cgcatagtaa gtaactcttt gcgctggaca tgatgtgcca
    54181 gcgtccctgg agacatacgc tcttcatagt tcgatgtgta ttgataatgt tgctctacgg
    54241 cagatgggga gtaattggtt gagatagcaa ccaccctgcc gctttgttct tctgcggtaa
    54301 caagagcata aagagagtta ttgctaccat tatacttgac gcgaaaatcg cgggtactca
    54361 gggtaatatc agaagctggg aaacactgtc gactttcttt atagtttgct tcacgcgcta
    54421 attttaacag ttcacggcta agtccagtgc tgttaagaag taatgacttt ctgatatccg
    54481 caagtgcctc cccttgctca atcatcaatg ctaactgggc tcctcgtggt gtggtaatta
    54541 catgttccag agtgataaat gttttctcac aatgatggca atacattctt ctctggcctt
    54601 gtgcagaata tccatatttt ttgagggacg tgcctccgca agttgaacat tggcaaatca
    54661 aacctctcca ggagtgattc acaatattac gataaatatt aagcgactgt tcagatatca
    54721 ctggaaacaa gtaaccacat tcacggcata aaatattttt accctgtgcg acataatcct
    54781 ggctattcag caaccccagg tttttacatc ctggtgtttt gcaacaatca acgtttacgt
    54841 tggtaaacat atccactccc tgcgaatagc cgtatcttga tattgttcaa cgcgaaaaga
    54901 acctgattca atgcgtagca ctgaatcagg cgggcatagc cctaaatggc atacattaca
    54961 tttattatga ttatcaggtt agccccaggt caccgcttct ggtttatcac ctctggcttt
    55021 cattagagaa tcattatcaa tatcagtttt atattttaaa aacttaccat acatctgttt
    55081 ttcaatatct ggttctttac cgatcagatt aagtgtttca taacgatcag atttcagatt
    55141 gtagagatat ttcggtttat tattgcgatc gataatcatc ttccagtcac catcacggat
    55201 cgcccattca tcggttggat catcctggaa tggcatatca atcccgaaaa ttaatggctt
    55261 ttcgcgtttc aatgcttttt gctcaagaac aggaaccagc gattcaccat cgaaagtacg
    55321 gtctgtaggt aatttgaagt tcatcatttt cgctaaagta ggcatccagt ccagaccata
    55381 aacgggtgta tctgaaacca ttccctgtgg tagatgttta ccatatttaa taatggctgg
    55441 aacacgaatt ccgccttccc aaaggttatc cttgcgaccg cgtaatccat ccgtttcccc
    55501 tgccaaattc agctcataca ctttgcgcgc ttcacgcgtt accggaccgt tatcactggt
    55561 aaaaataacg attgtgttat cttcttcacc catcgctttg attttatcca gcacttttcc
    55621 aacctgtgca tccagatagc tgatattggc ataatattcc cccacaccac gccagggttt
    55681 gtctgcccag tcgccataaa ataaatcagg atgctgcttc tgatacgcgc tcatatattg
    55741 tgagtacatg tcgaggtatt ttttgggcga agccaggggg ctatgcactt cggtaaaagc
    55801 aacatagagg aagaaaggct tgctgtcctt tttgttatcc agccagttga cgacttccga
    55861 actgacatac tcaccgctca ttttatcggc tcgtggagtg ggttgcccgt tacgtagcca
    55921 gcctgtcggg taaaccatgc cataacgcgg gcgttcttta gcgttatcca gcgtggcgtc
    55981 ggtaacaaag cccgccgtat taaccagtga gtaatcaaag cccatatctt gtgcttgtgg
    56041 ctgatcggtg cgatcgccgc ctgcattcag atgcagctta cccatcattg ccgtgtcgta
    56101 cccttgcgct ttgagtagat tagcaatcgt gagttcgtta cgccctaagg caacatcttt
    56161 gcctgaagga atccatgagc gaattccagt acgaaatggc atccggccgg ttaatagccc
    56221 tgcgcgtgaa ggtgaactta aaggagcggg ggcatagtag tcagtaaatt tgaccccttc
    56281 ctgggcaagc ctgtcgatat taggtgtttt aacgatctga tgaccatatg ttgctaaatc
    56341 gccataacct aaatcatccg ccataataat gactaaatta ggttgtttag cctctgcggc
    56401 taaggcagga ctaaaagcat tccctgtgca aactgcaagg ccgatcaaac tggccattaa
    56461 cgttttctgc ataattattc cctgataaaa attagaaatc ataaccaagc atataaatca
    56521 tttgatcgcc aaagccatcg tagcccagct tattatcgaa ataacgccac gtcacactgg
    56581 ctttccagcg aggatagaac ttccaggcaa tagttaaacc gccatttaac ccgttccggc
    56641 caaattgctg ctccgtgtaa gcatcgttcc tgtccagttc tatttcattc cagttagata
    56701 aaacaaattt ttcgtcaaat aacgtaaatg gtaatactgc tgtccagcca acaacataac
    56761 cattccaacc attcgtttga ccatatttag ctgatacgta gtcaccagat tgattatgca
    56821 atccaatata cggtttaaaa aagccccact cgccgttcca gccgaggtaa ccgaatccat
    56881 aaaacatgtc cagatcgtca ccccatgagt tatcccattg gccataaatt ttgccaaaga
    56941 atgtcatatc agagtcaaag agtctgacat gcgtcattgc tgaaaccgta tgatttctcc
    57001 ctgctacagc atgattgagt acattacttt cataaaacga gtaaaattct gcttctttaa
    57061 aatttacccc aaaatccgca gtcactttcc atacttcacc acggtgagtg tttacaaatc
    57121 cactattcca gtctgcataa cccgcattta ttgaaccaaa ggttccttta tattctaatg
    57181 aagaatatgc aggaaaatga tagatagctg ttatcagggt acacgcaata agaagtcgtc
    57241 ttttggcaga catataatat gttctccatt cattgttttt atgattccat aggaattaat
    57301 acaccagaca attcattttt attaagaatg accaggccag tatatttaaa gatgactatc
    57361 gaagcgtgat gaagatcgca gattactttt gcgaacgggc ggagggatat cgtgtttcag
    57421 aaaatgtgtc tgttttaaac atcacccttt gatttatagg catttttcat gatgaactta
    57481 gcattggctg ctgaacgtat ttctttgtct ctctatagta ttacagtaac ctttaataat
    57541 taatttcctt tagccattca cttttgatag aggaaaacac ctttttttgt gaatccctgg
    57601 atatatttaa gttaaataaa ttgatgtatt gaaatatttt tccgcaaaaa tgcgaggcgt
    57661 cttcagaaaa agaattgata aaacagaagt gaattaaaag cgcagtataa tagcggcggg
    57721 tgcttgaggc tgtctgtctc aggcattagc tgaacggcag atagagaaaa gccccgagtg
    57781 atattttacc atcaacccga ggcctcctat atgcagaaca catgtagagt gcctcttact
    57841 gaccgaaagg tcaaggagaa gagagcaatg aagcagcata aggcgatgat tgtcgccctg
    57901 atcgtcatct gtatcaccgc cgtagtggcg gcgctggtaa cgagaaaaga cctctgtgag
    57961 gttcacatcc gaactggcca gacggaggtt gctgttttca cggcttacga atccgagtaa
    58021 gagcaacggc ggggagtg
//
LOCUS       DAZGJT010000017        56619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZGJT010000017 DAZGJT010000000
VERSION     DAZGJT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.017"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1780)
                     /locus_tag="ACHKFK_001161"
     CDS             complement(<1..1780)
                     /locus_tag="ACHKFK_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO9051548.1"
                     /translation="MSQLDEAILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWKTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFA
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPMLTSATVIKLLHQRDENGEDRVTGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAGGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQHGRQVFMDFNRNPEPVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQQMNPLSISLYKMHGYDLITQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLAAPA
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSA"
     gene            complement(1777..3318)
                     /locus_tag="ACHKFK_001162"
     CDS             complement(1777..3318)
                     /locus_tag="ACHKFK_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO9051549.1"
                     /translation="MRKLSTAETLAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDSKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPSGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGTIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFSIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDRYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVALVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPFMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(3328..4104)
                     /locus_tag="ACHKFK_001163"
     CDS             complement(3328..4104)
                     /locus_tag="ACHKFK_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO9051550.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NNTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNEVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPHAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYSHQLLAGLMSATQDCQQATHAFAEKAGVSFHNQ"
     gene            complement(4114..4956)
                     /locus_tag="ACHKFK_001164"
     CDS             complement(4114..4956)
                     /locus_tag="ACHKFK_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9051551.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQSEDLQQIARQLAASNMNVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPMGTKDLYEAREQVKQGIRQLLPHSKEVGVPIALEPLHPMTAANRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(4966..5757)
                     /locus_tag="ACHKFK_001165"
     CDS             complement(4966..5757)
                     /locus_tag="ACHKFK_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO9051552.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDRPDADSVE
                     KLHATQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            5970..6617
                     /locus_tag="ACHKFK_001166"
     CDS             5970..6617
                     /locus_tag="ACHKFK_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9051553.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYISNP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKNNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTARE"
     gene            complement(6601..7239)
                     /gene="ytfB"
                     /locus_tag="ACHKFK_001167"
     CDS             complement(6601..7239)
                     /gene="ytfB"
                     /locus_tag="ACHKFK_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO9051554.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTHTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GADKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            7457..8077
                     /gene="fklB"
                     /locus_tag="ACHKFK_001168"
     CDS             7457..8077
                     /gene="fklB"
                     /locus_tag="ACHKFK_001168"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO9051555.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQTMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            8386..9798
                     /gene="cycA"
                     /locus_tag="ACHKFK_001169"
                     /gene_synonym="dagA"
     CDS             8386..9798
                     /gene="cycA"
                     /locus_tag="ACHKFK_001169"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO9051556.1"
                     /translation="MVDQVKVVADDQSPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTINLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPGLSDWVASLSVIILLLVLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFSLIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVLVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(9843..10505)
                     /gene="ytfE"
                     /locus_tag="ACHKFK_001170"
     CDS             complement(9843..10505)
                     /gene="ytfE"
                     /locus_tag="ACHKFK_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO9051557.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVDVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMEHISLENNVLFPRALA
                     GE"
     gene            complement(10613..11578)
                     /gene="ytfF"
                     /locus_tag="ACHKFK_001171"
     CDS             complement(10613..11578)
                     /gene="ytfF"
                     /locus_tag="ACHKFK_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9051558.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNMQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(11686..12546)
                     /gene="qorB"
                     /locus_tag="ACHKFK_001172"
     CDS             complement(11686..12546)
                     /gene="qorB"
                     /locus_tag="ACHKFK_001172"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9051559.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALTAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAADVKFIAYT
                     SLLHADSSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAVAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKPIT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            12635..13015
                     /gene="ytfH"
                     /locus_tag="ACHKFK_001173"
     CDS             12635..13015
                     /gene="ytfH"
                     /locus_tag="ACHKFK_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO9051560.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKMGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(13144..15087)
                     /gene="cpdB"
                     /locus_tag="ACHKFK_001174"
     CDS             complement(13144..15087)
                     /gene="cpdB"
                     /locus_tag="ACHKFK_001174"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO9051561.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYTQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            15277..16017
                     /gene="cysQ"
                     /locus_tag="ACHKFK_001175"
     CDS             15277..16017
                     /gene="cysQ"
                     /locus_tag="ACHKFK_001175"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO9051562.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQCYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(16007..16564)
                     /gene="ytfJ"
                     /locus_tag="ACHKFK_001176"
     CDS             complement(16007..16564)
                     /gene="ytfJ"
                     /locus_tag="ACHKFK_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9051563.1"
                     /translation="MTLSKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            16889..17095
                     /gene="ytfK"
                     /locus_tag="ACHKFK_001177"
     CDS             16889..17095
                     /gene="ytfK"
                     /locus_tag="ACHKFK_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO9051564.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(17173..18516)
                     /gene="paeA"
                     /locus_tag="ACHKFK_001178"
     CDS             complement(17173..18516)
                     /gene="paeA"
                     /locus_tag="ACHKFK_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO9051565.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(18839..19477)
                     /gene="msrA"
                     /locus_tag="ACHKFK_001179"
     CDS             complement(18839..19477)
                     /gene="msrA"
                     /locus_tag="ACHKFK_001179"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO9051566.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWESHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRRITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            19683..21416
                     /gene="tamA"
                     /locus_tag="ACHKFK_001180"
     CDS             19683..21416
                     /gene="tamA"
                     /locus_tag="ACHKFK_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO9051567.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGELEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSFDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            21413..25192
                     /gene="tamB"
                     /locus_tag="ACHKFK_001181"
     CDS             21413..25192
                     /gene="tamB"
                     /locus_tag="ACHKFK_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO9051568.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNVKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEAAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVSTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYAISMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     TVHVGDNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            25195..25536
                     /gene="ytfP"
                     /locus_tag="ACHKFK_001182"
     CDS             25195..25536
                     /gene="ytfP"
                     /locus_tag="ACHKFK_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO9051569.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            25686..27158
                     /locus_tag="ACHKFK_001183"
     CDS             25686..27158
                     /locus_tag="ACHKFK_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFO9051570.1"
                     /translation="MKKSLLAVMLTGLFALVSLPALGNVNLEQLKQKAESGEAKAQLE
                     LGYRYFQGNETTKDLTLAMDWFRRAAEQGYTPAEYVLGLRYMNGEGVPQDYAQAVIWY
                     KKAALKGLPQAQQNLGVMYHEGNGVKVDKAESVKWFRLAAEQGRDSGQQSMGDAYFEG
                     DGVTRDYVMAREWYSKAAEQGNVWSCNQLGYMYSRGLGVERNDAISAQWYRKSATSGD
                     ELGQLHLADMYYFGIGVTQDYTQSRVLFSQSAEQGNSIAQFRLGYILEQGLAGAKEPL
                     KALEWYRKSAEQGNSDGQYYLAHLYDKGAEGVAKNREQAISWYTKSAEQGDATAQANL
                     GAIYFRLGSEEEHKKAVEWFRKAAAKGEKAAQFNLGNALLQGKGVKKDEQQAAIWMRK
                     AAEQGLSAAQVQLGEIYYYGLGVERDYVQAWAWFDTASTNDMNLFGTENRNITEKKLT
                     AKQLQQAELLSQQYIEKYAPEAWARMQKLKAQSAVKTGNK"
     gene            complement(27572..28102)
                     /gene="ppa"
                     /locus_tag="ACHKFK_001184"
     CDS             complement(27572..28102)
                     /gene="ppa"
                     /locus_tag="ACHKFK_001184"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO9051571.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            28412..29368
                     /gene="ytfQ"
                     /locus_tag="ACHKFK_001185"
     CDS             28412..29368
                     /gene="ytfQ"
                     /locus_tag="ACHKFK_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO9051572.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            29508..31010
                     /gene="ytfR"
                     /locus_tag="ACHKFK_001186"
     CDS             29508..31010
                     /gene="ytfR"
                     /locus_tag="ACHKFK_001186"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO9051573.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALAGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEIEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKLQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHTEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            31024..32046
                     /gene="ytfT"
                     /locus_tag="ACHKFK_001187"
     CDS             31024..32046
                     /gene="ytfT"
                     /locus_tag="ACHKFK_001187"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO9051574.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RNRDKT"
     gene            32033..33028
                     /gene="yjfF"
                     /locus_tag="ACHKFK_001188"
     CDS             32033..33028
                     /gene="yjfF"
                     /locus_tag="ACHKFK_001188"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO9051575.1"
                     /translation="MIKRNLPLMITIGVFVLGYIYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPVYDTLSSLAWKIPGGG
                     RLSTMGLLMLAVVVIGILLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(33061..34059)
                     /gene="fbp"
                     /locus_tag="ACHKFK_001189"
     CDS             complement(33061..34059)
                     /gene="fbp"
                     /locus_tag="ACHKFK_001189"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO9051576.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            34235..35608
                     /gene="mpl"
                     /locus_tag="ACHKFK_001190"
     CDS             34235..35608
                     /gene="mpl"
                     /locus_tag="ACHKFK_001190"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO9051577.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(35766..36317)
                     /gene="yjgA"
                     /locus_tag="ACHKFK_001191"
     CDS             complement(35766..36317)
                     /gene="yjgA"
                     /locus_tag="ACHKFK_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO9051578.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            36411..37763
                     /gene="pmbA"
                     /locus_tag="ACHKFK_001192"
     CDS             36411..37763
                     /gene="pmbA"
                     /locus_tag="ACHKFK_001192"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO9051579.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPYAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGAVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIVKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            37946..38332
                     /gene="cybC"
                     /locus_tag="ACHKFK_001193"
     CDS             37946..38332
                     /gene="cybC"
                     /locus_tag="ACHKFK_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO9051580.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AVQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(38377..38841)
                     /gene="nrdG"
                     /locus_tag="ACHKFK_001194"
     CDS             complement(38377..38841)
                     /gene="nrdG"
                     /locus_tag="ACHKFK_001194"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO9051581.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(38999..41137)
                     /gene="nrdD"
                     /locus_tag="ACHKFK_001195"
     CDS             complement(38999..41137)
                     /gene="nrdD"
                     /locus_tag="ACHKFK_001195"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO9051582.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEICKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDTSRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(41531..43186)
                     /gene="treC"
                     /locus_tag="ACHKFK_001196"
     CDS             complement(41531..43186)
                     /gene="treC"
                     /locus_tag="ACHKFK_001196"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO9051583.1"
                     /translation="MTHLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRYPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTSYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWTNGDNAGFTAGEPWIGLGDNYQEINVEAALA
                     DESSVFYTYQKLIALRKQEAVLTWGDYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     TQPWQPGKMLGNWQLVMHNYEEASPQPCTMTLRPFEAVWWLQK"
     gene            complement(43236..44657)
                     /gene="treB"
                     /locus_tag="ACHKFK_001197"
     CDS             complement(43236..44657)
                     /gene="treB"
                     /locus_tag="ACHKFK_001197"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO9051584.1"
                     /translation="MMSKINQTDIDRLIEMVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GIFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(44776..45723)
                     /gene="treR"
                     /locus_tag="ACHKFK_001198"
     CDS             complement(44776..45723)
                     /gene="treR"
                     /locus_tag="ACHKFK_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO9051585.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            45908..45961
                     /gene="mgtL"
                     /locus_tag="ACHKFK_001199"
     CDS             45908..45961
                     /gene="mgtL"
                     /locus_tag="ACHKFK_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO9051586.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            46102..48798
                     /gene="mgtA"
                     /locus_tag="ACHKFK_001200"
     CDS             46102..48798
                     /gene="mgtA"
                     /locus_tag="ACHKFK_001200"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO9051587.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNAAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNLAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(49004..49390)
                     /gene="ridA"
                     /locus_tag="ACHKFK_001201"
     CDS             complement(49004..49390)
                     /gene="ridA"
                     /locus_tag="ACHKFK_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO9051588.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(49463..49924)
                     /gene="pyrI"
                     /locus_tag="ACHKFK_001202"
     CDS             complement(49463..49924)
                     /gene="pyrI"
                     /locus_tag="ACHKFK_001202"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO9051589.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(49937..50872)
                     /gene="pyrB"
                     /locus_tag="ACHKFK_001203"
     CDS             complement(49937..50872)
                     /gene="pyrB"
                     /locus_tag="ACHKFK_001203"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO9051590.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(50876..51010)
                     /gene="pyrL"
                     /locus_tag="ACHKFK_001204"
     CDS             complement(50876..51010)
                     /gene="pyrL"
                     /locus_tag="ACHKFK_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO9051591.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(51100..51588)
                     /locus_tag="ACHKFK_001205"
     CDS             complement(51100..51588)
                     /locus_tag="ACHKFK_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HFO9051592.1"
                     /translation="MKEYDDYSAKEKKQLAVCQRLITEKSYLSQEEIRRDLQNHGFDS
                     ISQSTVSRLLKLLGVIKIRNTKGQKIYSVNPQLLPTPDAGRSVAEMVLSVEHNGEFIL
                     IHTVAGYGRAVARILDFHALPEILGVIAGSNIVWVAPRVVKRTALVHKQINYLLKLNI
                     YS"
     gene            complement(51704..53107)
                     /locus_tag="ACHKFK_001206"
     CDS             complement(51704..53107)
                     /locus_tag="ACHKFK_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HFO9051593.1"
                     /translation="MGKFKFPTAYTILFVLIALVAAMTWIVPAGKYQMVTNAALGKEV
                     PVAGTYAPVEAQPQGITAVLLAPIDGLYNHQTYTAGAIDVALFVLIIGGFLGVVNKTG
                     AIDAGIERVTTRLNGREEWMIPILMALFAAGGTIYGMAEESLPFYTLLVPVMMAARFD
                     PLVAAATVLLGSGIGTLGSTINPFATVIAANAAGIPFTQGILLRVILLLVGYVICVYW
                     VMRYARKVRNSPESSIVADKMAENQAHFLGNRSETMLEFTPTRKAILILFAASFAFMI
                     YGVAVLGWWMAEISAVFLAAAVIVGVIARMGEETFTSTFIDGARDLLGVALIIGIARG
                     IVVVMDNGMITHTILHSAENLVSGLSTTVFINVTYWLEVLLSFLVPSSSGLAVLTMPI
                     LAPLADFAHVQRDLVVTAYQSASGVVNLITPTSAVVMGGLAIARVPWVRYLRWVAPLL
                     LILTMLNMIVLSIAAMI"
     gene            complement(53164..54168)
                     /gene="argF"
                     /locus_tag="ACHKFK_001207"
     CDS             complement(53164..54168)
                     /gene="argF"
                     /locus_tag="ACHKFK_001207"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO9051594.1"
                     /translation="MATSLKNRNFLKLLDYTPAEIQYLIDLAINLKAAKKSGNEKQTL
                     VGKNIALIFEKSSTRTRCAFEVAAFDQGAQVTYIGPSGSQIGHKESMKDTARVLGRMY
                     DGIEYRGYGQNIVEELGEFAGVPVWNGLTNEFHPTQILADLMTMLEHAPGKTLPELSF
                     AYLGDARNNMGNSLMVGAAKMGMDIRLVAPKSFWPDEALVTHCREIASVTGARITLTE
                     DVEEGVYDVDFLYTDVWVSMGEPKEAWAERVSLMAPYQINQQVITATRNPEVKFMHCL
                     PAFHNEHTTVGREIEMAYGLKGLEVTEEVFESAHSIVFDEAENRMHTIKAVMVATLGD
                     "
     gene            complement(54192..55124)
                     /gene="arcC"
                     /locus_tag="ACHKFK_001208"
     CDS             complement(54192..55124)
                     /gene="arcC"
                     /locus_tag="ACHKFK_001208"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9051595.1"
                     /translation="MENKPTLVIALGGNALLKRGEPLEAEIQRKNIDLAAKTIAQLTQ
                     HWRVVLVHGNGPQVGLLALQNSAYAHVAPYPLDILGAESQGMIGYMLQQALKNQLPQR
                     EISVLLTQVEVDANDPAFSNPTKYIGPIYDHAQTQVLQAEKDWVFKADGHSFRRVVPS
                     PQPKRIVERDAIQTLIAHDHLVICNGGGGVPVVEKADGYHGIEAVIDKDLSAALLASQ
                     IHADALLILTDADAVYLDWGKPTQRPLAQVTPELLNEMQFDAGSMGPKVTACAKFVSQ
                     CRGIAGIGSLADGPEILAGDKGTLIRLDTPHNHA"
     gene            complement(55135..56355)
                     /gene="arcA"
                     /locus_tag="ACHKFK_001209"
     CDS             complement(55135..56355)
                     /gene="arcA"
                     /locus_tag="ACHKFK_001209"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0019546 - arginine deiminase pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HFO9051596.1"
                     /translation="MEKHYVGSEIGQLRSVMLHRPNLSLKRLTPSNCQELLFDDVLSV
                     ERAGEEHDIFANTLRQQGIEVLLLTDLLTQTLDIPEAKSWLLETQISDYRLGPTFATD
                     VRTWLAEMSHRDLARHLSGGLTYSEIPASIKNMVVDTHDINDFIMKPLPNHLFTRDTS
                     CWIYNGVSINPMAKPARQRETNNLRAIYRWHPQFAGGEFIKYFGDENINYDHATLEGG
                     DVLVIGRGAVLIGMSERTTPQGIEFLAQALFKHRQAERVIAVELPKHRSCMHLDTVMT
                     HIDIDTFSVYPEVVRPDVNCWTLTPDGHGGLKRTQESTLLHAIEKALGIDQVRLITTG
                     GDAFEAEREQWNDANNVLTLRPGVVVGYERNIWTNEKYDKAGITVLPIPGDELGRGRG
                     GARCMSCPLHRDGI"
BASE COUNT        13607 a        14261 c        14949 g        13802 t
ORIGIN      
        1 cggcagaagt cagcgccata tgccgccaca taaacagctc ggcaacttca cccacatgtt
       61 tgattgccaa tccatgccgt tgcacaaatt cactcaatag cagggcatca cgagtggcgc
      121 gtcggacttt atcggcatgg caaataaatc ccgcatggtc agacattcgt gcctgaattt
      181 tttccctcac aaccgacaac ggcatcccgg ttccgttatc gtgcgcctga gtaatgatct
      241 ctcttataga agccagtgcc ggagctgcca gctgtgcaat atctccatca atattccgct
      301 tttgcgtgca accaataaag cgcgccagac gaacagcaaa aacctgcccg gcattcaatg
      361 ccgcaccgcc aggtcgggtg acgccgtgtg tgccagcaac ttcccccacc gcaaaacaac
      421 cgggcagaga tgtttgtccc cagatatcga cttcaatgcc gccattcatg tgctgattat
      481 tcatggcaaa ctgcaatggc tgcgtgatga gatcgtaacc gtgcatctta tacagcgaga
      541 tagacagagg attcatttgt tgcaggcgtt cgatgggcga tggtgccaga gcgtcgttat
      601 tttccagata cgcgcgaacg tcgtcgtcca gtcgatctaa tgagaacggc aggtcacccg
      661 gcacaggttc tggattacga ttgaaatcca taaatacctg acgaccatgt tgctgctctt
      721 gcgctactgc catatctaac aagctggagc caaaatccat tacccgcgtg gcgtggaacg
      781 gccactgata gcctttacgg aaaatgtttg aagccagctc ctgggtggtg cgatagtaat
      841 ccgcgaggaa gttatactcg ttacccccgg catccacgga atagatgtat gggatcacct
      901 gtacataagt cccggacaaa ttccacggaa acgtgctgcg cggtgtgccg ataccaaact
      961 ggctttcagt cagattggtt agcgtcagac cttcctccag cgccagcccc agcgagccga
     1021 aacatttgtg tggatacaca ctgtcgcgat aaagctcgcc aggcccgcct gttgccagta
     1081 ccacattggg cgcagtcaca attgccagcc cccatgggtt atgggcgcga tgaccggtcg
     1141 cgaggattgc ccccgtcacg cggtcttcgc cgttttcgtc acgctgatgt agcagtttaa
     1201 tcactgttgc actggtcaac attgggatag caagacgctg tacttcttcc agcagcactt
     1261 tcaccattaa ccttgaggta cgcggcccac acgaggttgc acgcccggct tcgtcgtggt
     1321 cggtttgata acgaagaatc gcgccatagc gatcttccgg taattccaga ccaagatatt
     1381 gcaacccgcc aagagtgtgc agagaaccta ccgcttcaac ataagcggtg tcgtgatcca
     1441 tcgccccacc gctcgccagt gcctctgcca gtttggcgaa gttgtcgccg ttgcccgcgg
     1501 tagcggcggt aaacagtgtt tgtttatcag agccagaaca tgccgacgtc cccatatata
     1561 acccggcggt ggcgattaac acattttgct ggcggcgttt aagttccaca gctgcacgta
     1621 gcccggctgc accactgccg actacaaggg catctgtttt ccagagtggg taatcaacac
     1681 cgtctaccgt gacaacccat gcgggttctg cctgtgcaaa accctttggg aaagtaacgt
     1741 gcgtcagtgc atcaaggatg gcctcatcaa gctggctcat gcatcctcca tcggcgtaaa
     1801 caggctgctt tccatcagac gtgggtgctt gataattgga cggaaggcca tgaatggcag
     1861 aatgtcgcgc tcaatctcta cgccaggggc gatctcaatt aattcaacgc cttctgccgt
     1921 taactggaat accgcacgtt cggtgatata cagcacttcc tgaccgcttt gctgtgcgta
     1981 ggcggcgcta aaggtgattt tttccaccag ggcaaccagt ttagggattt gacctgactg
     2041 ggcgatatgc aagccatctg gcgttacatc aatcttgctg cctttggcgt cgaacgtacc
     2101 gcaaaacacc actttacggg cgttttgtgc aatttcgaga aatccgccgg ggccaatgag
     2161 attgccatta agatgggata cgttgacgtt accgtaccga tccatctctc ccatgccgag
     2221 gaaggtgata tcgataccgc cgccactgta gaattcgaat tgatcgagtg atggaataat
     2281 ggcatcggcg ttacgggcgc agccgaacag agcatcatcc agcatcatgc cgttatgcac
     2341 cccctgttct acactcagcc acaaactttg ataaggcacg ccttcgcgca gcgcaacccc
     2401 aggaataccg cccggaatac taaagccaaa gttggttgaa gcacctgcca ccagttcccg
     2461 cgctgcccga cgggcaatca ggcgacgcac cggatgacta accagttcaa tagcgtcgtt
     2521 agaacttaga cggcgatgtt gaccgctgat cgtcaggtcg taaccgccaa gataggtttg
     2581 cggttgttcg cggtgctcaa cgatgccatc gaccagaatt cctggtagct tgacccgacg
     2641 tggttcaatg gtacctgctt ccagcacatc gcggacctgt acgaacactt tgccgccgct
     2701 gttgtgtgct gccagcgcca tcgaatagct atccatatca atcgcttctt cttcgaggct
     2761 aacgttgcct cgtggatcgg cataagtgcc acgcaaaatc gcgtaatcca ctttgaaagg
     2821 gcgataacga agtttggtct caccgtcgat ttcaatcagt tctaccaggt cgtcggttgt
     2881 gcattcattc gacttaccgc cgccattgcg tggatcaaca aacgatccca gcccaacgtg
     2941 ggtaaaaagc cccggacgtc cggcaccgat ctcccgcagc agtgcctgaa ttacgccact
     3001 aggaaagcag taggcttcaa tcgtgttatt ttttaccagc gcctgcattt tgggcgacca
     3061 ggtaaagtgt ccggcaataa tgcgtttgag catttcggcg tgagcaaaac ggttagtgcc
     3121 tttgctgtcg cgatcgccaa tacccagcga gtggattaat gtcagatcgc gcgggtgtcc
     3181 ggtttgcaga aagcgcgctt caatggcggc cagaatatgg tcagcttcca ccatgccgcc
     3241 gccgttaccg ctaatggcaa tggttgcgcc gtcctgaatt tgcgccgcca gtgtctcggc
     3301 ggttgataac ttacgcatac aatttcctta ttgattgtgg aagctcacgc cggctttttc
     3361 ggcaaatgcg tgcgtcgcct gttggcaatc ctgagtggcg gacattaatc cagccagtaa
     3421 ctggtgggag taatcggcgt gtttattttc cagcgccaga atcagttgct taatgtgtgc
     3481 cagtgccacc ggaccacatt tttccagctg cgctaactgg ttcgccatcc agctttccac
     3541 ctgttctttt tctaccactt catcaattaa attcgccgcc tgagcttcct gcgccgtcag
     3601 gcgtttaccc agcatcagca tctggcgacc gaccaccggg ccaacctgat taagcacgcg
     3661 ttcaatgccc atccagccag gcaccatgcc gagcatcact tcaggattag agaattttgc
     3721 gtgggggcga gcgatacgaa tatcacagca caaggcgagt tcaatcccac cgccaatggt
     3781 atgaccgttg agatttgcaa ccgttaattg gggtaaattg cgcaggcgat taaaaacctc
     3841 gttaccgcgt ttaatccatt ttctgcccat atctaatggc gaataggccg accacgattt
     3901 aatatcgccg cccgcacaaa agaatttatc gccggttgcc gttaattcga ctaatcgtaa
     3961 tgtggtgttg ttttcaattt catttaaata atggtcgaga gattcgatca tttcttcatt
     4021 aatagcatga catttatctt cacggttcaa tgtcaggcgg caagaagccg ccgctctgct
     4081 gaacagaacg ggttggtcac tcatgattat tccttagcaa taatgcgcga tgcgatcgac
     4141 gctaatatcc agcgtgctgt taatatcttt ctgccaccag tacggtgaga atatttccag
     4201 ttcaatagcg ccattaaatc cggcgttttc aaccagccga cgaattgacg gaatattgat
     4261 gacaccatct cccggcattc cacggtcatt gaccagatcg gtggtcggca ctaaccagtc
     4321 ggaaacatga aacgcgagga tgcgttttcc ggcacgcagg atctgactgg cgagatccgg
     4381 gtcccaccag acatggtaga catctaccgc cacgccgagg ccaaattcgc catcgggatc
     4441 gagttcatca caccagtcca gcgcctgacg taacgtgcac agacaagaac gattggcagc
     4501 agtcatcggg tgcagtggct ccagtgcaat tggcacgccg acctctttcg aatgtggcag
     4561 caactggcga atcccttgtt tcacctgttc ccgtgcttcg tacagatctt ttgttcccat
     4621 tggcagaccg ccaaccacct gcatataaca ggcggcgttc agtacagcgg cgtcgtccag
     4681 cgcccggcgg ttatcgtcaa tcgccagttt gcgctccgcc agcgtcggtg cggtgtagta
     4741 ggtgctacgg cacaaaccgg aaacgttcat gttactggca gcaagctggc gggcgatttg
     4801 ctggagatct tcactctgca actccctgcg ccagggggca atcgcgccaa taccgcgtgc
     4861 ggcgcaggca tcaataatgg cgggtagcgg cgttttatag cccagtgtcg cggtattgat
     4921 actgaataaa tccgggcgtt gtaacagatc tctcatgatt cagcctcaga agcgcggcat
     4981 acttaagccg ccgtcgatgg cgaccgcctg accacaggtg taaataagtt taccttccgc
     5041 catcgcgcgg acagtggaag caatatccgc cggataaccc caacgtcccc acggcaccag
     5101 acctttggca atcagttcgt cgtaataggc cgttgccgga atagtcatat cggttttgat
     5161 gagaccagga cgcacttcat atacgccaat ttgttcatta caaaggcgag cagcaaacag
     5221 acgagcagcg gcagagacgg cagttttggc aatggtatat tcgccgcgat tcatcgccag
     5281 catgatggcg ttgatggaac tgacaaaaat aatcgagcgg tgcggcagtt ctgcttccgg
     5341 tttcggctgt gccagtaagc gcttactgaa tgcctgagca aggaagaacg gtgcgcgggt
     5401 gttaatggca atattttggt cgaagctgtc tggttcgagg tcgagaaggt caccgcgttt
     5461 tttcactgaa atgcctgcgt tgttgagcag gcagtccaga cgtccccact ggttttgcgc
     5521 tgcgtcgagc atctcttcat gcagggagag atcgcccaca tcagcaggga agcaaatcac
     5581 ttccacgcct tccgccagac attcttgttg tgtggcatgt aatttttcta cgctatccgc
     5641 atccggacga tcattaatca gcagattaaa tccgccacgc gcaagctcca gcgcacagcc
     5701 ctttccaata ccacgagcgg ctccggtaat cacggcaaca ggacgagtgg atttcataca
     5761 atagctctct ttttttgaat tttgtttcct tagtgatgat tagcttgcga ctggaggata
     5821 aaaagatcaa ccatctagtt ggttgatatg cgatggcgat cacaggacat ttgataacct
     5881 gatgaaagta caagtttttg cccttttttc tgaagctgaa atcaaatcag gttatgatag
     5941 tcaccacgaa gaggtcgtgg ggaagcgtta tgtatcttga taccagcaac gtacagagcc
     6001 tgaaagaaaa gctgctgtta tgcgcggtta atgaatttgc cgaatatggt tacgaagggg
     6061 ccagagtcga taatatcgtc aaggccgcgg ggtgcagtaa acagacggtt tatcaccact
     6121 ttggtaataa agaaaattta tttatcgaag tactggaata tacctggaac gatattcgac
     6181 aaaaagaaaa ggcccttgat ttctcagatc ttccgccaca gaaagccatt gagaaaataa
     6241 tcgattttac atgggattat tatatttcta atccctggtt tttaaaaatt gtgcatagtg
     6301 aaaatcaaag taagggtgtg cattatgcaa agtcacagcg tcttttagaa attaaccatg
     6361 cgcacttgca gttaatggaa tccttattgg atgaagggaa gaaaaacaat atttttaagc
     6421 cagatatcga cccattgcag gtgaatatta atattgctgc gcttggggga tattatttga
     6481 tcaaccagca tacgcttggt ctggtttatc acatcagtat ggtttcaccg caggcactgg
     6541 aagccagacg taaggtcatc aaagagacga tccttagctg gcttttggtt gacccttcat
     6601 ctaccgcgcg cgaataaaac tgccatccgg ctggcgggta aacaggacct gttgattatt
     6661 ccccgtatca atggttaagc ccgtcaccac gccgctggcg ttctggcgga ttttcaccat
     6721 ctgcccgttt tgcaaattac tcagtggctt atccgcgcct tctacctgcg ccatggcata
     6781 gacatcggta gcgggcagtc cgtggtcgcg gaacagctgc gccatcgttt taccgggttc
     6841 gacacgatag gagcgccatt gattatcaat tccgctatct ggctggaatg gttgcgtatg
     6901 ggtggtttgc ggctgctctt ctggttggcc ttcctgtatt ggttctggag cgaccggagc
     6961 cacctgatca ggatcatttt gtggcgtgac taattgcgcc cgcagctgtt cttctgtcgg
     7021 tggttgtgat tgcgactgaa tatctaactg cgcttcacgc gtcaccaccg gcgcgttggg
     7081 cgtatcatca gatggaagca gaaatccgac caccaataca atggcggcaa taatgatccc
     7141 ccgacgatgc atcggcggca gcgggtccat gaagcgaaaa ttgtccggcg cgtgccagac
     7201 tttctccagg gttggtttta gttcaaagcg cccgggcatg gtttcctcct gctccgcgtc
     7261 ttgttcctca atcatagcct atgaataagc taacgctatg atgtccgtgg taaacccgcc
     7321 tttattatat tcatacggga tattgctatt gtttcttttt ccccgggatt tgtcatcatt
     7381 cccgcgacaa agttttaccc aaagaagtgt ggctgatatg ctgcccgcta ctttataccc
     7441 taagaaagga aatacgatga ccaccccaac ttttgacacc atcgaagcgc aagcaagcta
     7501 cggcattggt ttgcaggtag ggcaacaact gagtgaatct ggcctggaag ggctgctgcc
     7561 agaagcactg gttgcaggca ttgccgatgc gctggaaggc aaacatccgg ctgttccggt
     7621 tgatgtggtg caccgcgcgc tgcgtgaaat ccacgaacgc gccgatgccg ttcgtcgtca
     7681 gcgtttccag acgatggctg ctgaaggtgt gaaatacctg gaagaaaacg ccaaaaaaga
     7741 aggtgtaaat agcaccgaat ctggcctgca attccgcgtg atcaaccagg gcgaaggcgc
     7801 aattccggca cgtaccgacc gcgttcgtgt tcattacacc ggaaaactga tcgacggcac
     7861 cgtgtttgac agctccgttg ctcgcggcga gccagctgaa ttcccggtta acggcgtgat
     7921 ccctggctgg attgaagcac tgactctgat gccggtaggt tctaaatggg aactgactat
     7981 cccgcaggaa ctggcatatg gcgagcgtgg cgcaggcgcg tccatccctc cgttcagcac
     8041 cctggtgttt gaagtcgaac tgctggaaat cctctaagca gcgcattctg ttcccctcga
     8101 accagagggg agcaggcatt cagcaataaa cccttcagtt tgccaaacgg cgctatttta
     8161 tgttgcaaag accctgtaag cgtgtatttt tgtgagctgt ttcgcgttat caccgtgata
     8221 tgacactcac tttaaacata aaattaacat tagatctaaa tcttagtatt catcccgcgt
     8281 attgttacct aatatcgatg agtcccgata cagattcgtc gtatcataga ctgactaaag
     8341 gccgtagagc ctgaacaaca cagacaggta caggaagaaa aaaacatggt agatcaggta
     8401 aaagtcgttg ccgatgatca gtctccggct gaacagtcgc tacggcgcaa tctcacaaac
     8461 cgacatattc agcttattgc cattggcggt gccattggta cagggttgtt tatggggtcc
     8521 ggcaaaacga ttaaccttgc cgggccgtcg atcattttcg tttatatgat catcggtttt
     8581 atgctctttt tcgtgatgcg ggcaatgggg gaattgctgc tttcgaatct ggaatacaaa
     8641 tcttttagtg acttcgcttc cgatttactc gggccgtggg caggatattt caccggctgg
     8701 acttactggt tctgctgggt tgtaaccggt atggcagacg tggttgccat taccgcctat
     8761 gcgcaattct ggttccctgg gctttctgac tgggttgctt cgttatccgt gatcattctg
     8821 ttactggttc taaacctcgc cacggtaaaa atgttcggtg agatggagtt ctggtttgcg
     8881 atgatcaaaa tcgtcgccat agtgtcgctg attgttgtcg gcctggtcat ggtggcgatg
     8941 cactttcagt caccgaccgg tgtggaagcg tcatttgcac atttgtggaa tgacggcggc
     9001 tggttcccga aaggcttaag tggcttcttt gctggattcc agatagcggt tttcgctttc
     9061 gtggggattg agctggtagg tacaacagct gcggaaacca aagatccaga gaaatcactg
     9121 ccacgcgcga ttaactccat tccgatccgt atcattatgt tctacgtctt ctcgctgatt
     9181 gtgattatgt ccgtgacgcc gtggagttcg gtagtcccgg agaaaagccc gttcgttgaa
     9241 ctgtttgtgt tggtaggttt gcctgcggct gccagcgtga tcaactttgt ggtgctgacc
     9301 tctgcggcgt cttccgctaa cagcggtgtc ttctctacca gccgtatgct gtttggcctg
     9361 gcccaggaag gtgtggcacc gaaagcgttc gctaaactct ctaagcgcgc agtacccgcg
     9421 aaagggctga ccttctcttg tatctgtctg ctcggcggcg tggtgatgtt gtatgtgaat
     9481 cccagcgtga ttggcgcgtt cacgatgatt acaaccgttt ccgcgattct gtttatgttt
     9541 gtctggacga ttatcctttg ctcgtacctg gtgtatcgca aacagcgtcc tcatctgcat
     9601 gagaagtcga tctacaagat gccactcggc aagctgatgt gctgggtatg tatggcgttc
     9661 tttgtgtttg ttctggtgtt gttgacactg gaagatgaca cccgccaggc gctgctggtt
     9721 accccgctgt ggtttatcgc gctggggctg ggctggctgt ttattggtaa gaaacgggct
     9781 gctgaactgc ggaaataatc gcattatcat gctggatggc gcaatgccat ccagctttta
     9841 gatcactcac ccgccagcgc gcgcgggaat agtacattgt tttccagact gatgtgctcc
     9901 atcaggtcat caatcagttc attaatgccg ttatacatcg ctttccaggt ggtgcaggct
     9961 tccggcggtg gtgtgacgtt attggtggtg tgtttgatca cttccagcag ttcgcccgct
    10021 tcatcgtgct cgctttccat tacgctgatt ggccccattg cctggctgcc catgccttgt
    10081 ttaatcatcg ggaagagaat ttgctcttct ttcatcatgt ggctggaaag ctcttcatgc
    10141 agcatagtca ggtattttgt cagccctttt ggcacacttg gtttgtcggc gtgaacgcgc
    10201 tcgactttag tcgcttgcag gatcagctcc ggcagctgct cgcgatggcg atcgtgatag
    10261 cgcacaataa tgtggtcgat gatttccgcc agcggggcgc tacgccagtc tttctcaatc
    10321 ggttgttcag caagctttgc cagttcggct tcaatgacgt caacatccag ttctttacgt
    10381 gccgccgcgc gcgccagcgt ctgcttaccg ccacagcagt aatccatatc atatttacga
    10441 aacagagccg aagcgcgagg aatagagagc gccagttcac ctaaaggttg gtcgcgataa
    10501 gccatagctg atacctcatt cttaataaga taagatgcat tttaaatgca tctttaaggc
    10561 aaaagctata acccttacgt agcagaggct tatttaactc actgccagcg tgtcaacttt
    10621 ctgagacaga ttcagttaaa ggtttttctg gttttgctct gaccgcgatg accacgccaa
    10681 tcactaacag cgcgataccg ctcagcgtca ttagcggcgg catttgctgg cggagtaaaa
    10741 aggtgtacag caaacctgcc agcgtttcga aaacaatcag cggcccgaga atcactgtcg
    10801 gtaatcgctg gctggcgacg ttccagcaga gtgcgccaac ccatgagcaa agcacggcta
    10861 tcgcaaccat cagactaata aacaccagcg gacgggggcc aaagggtaag gagaagtccg
    10921 gcgtttgcat attcagccag taacaggcga cgagatagcc gatgagagaa accggcagcg
    10981 tgaccagcgc ctgcgccgtc gcccacatca tcggatgttt gtcgggattt tcccgcagcc
    11041 agcgggcgtt gcgcagggca taccatgccc agcagaccac ggaaactaac gctagcacga
    11101 tgcctgaggt ataacgtgcc cagtcaaaat cggggagtcc gtggtttaac tcagcaatat
    11161 tcacacacgc caggccgatg ccaatacaaa ccagtgccgg ggcgagtttt ccccatgcga
    11221 gtttgccgtc gcgctggcta taaagcagat tggcaaaaac gggaatcacc accggcaggg
    11281 tgccgataat catcgtggaa acaggcgcgc cagtacgttg aatggcactt gcaaggcaga
    11341 agtaatagat aaggttgccc atcatggtga gcatcaaggc ggtaagccag tcgcgacgcg
    11401 ccaactgacg cagacgcacg cgtcccagcc aggcaatggg cagcgcaatt aaccctaacg
    11461 ccagataacg ccccatcgac tgcaacatcg ccgggtattc cggtacgatc aacggcccga
    11521 caaaaataag cccccacatc aaccctgcta acagggcgta cagcacgccg ctaatcatta
    11581 ctggcatcca ttggtctgtc agaagaaagt tcaggaagca gacatgattg ctcccggcat
    11641 cccgtcatta tcagagagga tgcgggagga tgccacttta attaactagt tattaacatt
    11701 aaaaagatgg cttacgcttt cggctaacgt tgtcgttggg cggccaatca atttgctaag
    11761 cgttttgctg tcatcaaaca gaccgccttt cgatgcgcca acgtcagaat ccgccagcat
    11821 atccgccagt ccatcaggca gaccgacgct tttcagcgcc gcggcgaaat cagcttcgct
    11881 caggttttga taggtaatcg gtttgccgct ctgtttggtg agttctgccg ctaactgtgt
    11941 cagcgtccag gcactatcgc ccgccagttc gtaaaccttg ccttcgtgac cggcttcgct
    12001 aatcacgcgt gccgcagcta ccgcataatc agcccgcgtt gctgaggcaa ttttgccatc
    12061 gcccgcggca ccgataaata cgccgtgttc cagtgctgcc ggggcgctgg cgaggtagtt
    12121 ttcactgtac cagccgttgc gcagcagggt gtaaacgatg ccagaatcag ccagcatttt
    12181 ctccgtctcg atgtgctcat cggcgaggcc gagcggggag gaatcggcat gtagcaggct
    12241 ggtataagcg ataaatttca cgtcggccgc ctttgcggca ttaataacat tacgatgctg
    12301 cggggcacgt tgacccactt cgctggaaga gatcagcagt agtttttcca ctccctgaag
    12361 cgcagatgtc agtgctgctt catcgccgta gtcagcctga cgcacggtaa tgccttgtgc
    12421 tgtcagtgcc tgtgcttttg ccggattacg aacgatagcc actatttggc tggcaggaac
    12481 cgttttcatc aaggattcaa taacatagtg accaagttgg ccagtggcac cagtaatagc
    12541 gatcatggga agtctccatc gttttgtctt gtgtttaaat acgctaacac ctaaacttac
    12601 ttttagtaag tacgtacaaa aaggtaagta tgaaatgagt caggttagcc tgtcgcaaca
    12661 actgaaagag ggaaatctct ttgcggaaca gtgcccgtcg cgcgaggttt tgaaacacgt
    12721 caccagccgt tggggggtgt tgattctggt ggcgctacgc gaaggtactc atcgctttag
    12781 cgacctgcgg cgcaaaatgg gcggggtgag tgaaaagatg cttgcgcagt cgttacaggc
    12841 attagaacag gatggttttc ttaaccgtat cgcgtatccg gtggtgccgc cgcatgtgga
    12901 atatagcctc acgccgctgg gcgagcaggt gagcgaaaag gttgctgcac tggcggactg
    12961 gattgagttg aatttgcccg aggtgttagc ggtgcgggat gaacgtgcgg cataactgcc
    13021 ggatgcgctg cgcttatccg gactacggtt catggcttgt agggcggata agatgcgtca
    13081 tcatcgcatc cgccaataaa tgccggatgc ggttgatgat ttgtaggccg aaaaagaagt
    13141 gttttactta ctcagatcca cctgataaat cgcaaacccg atatcatcgg tcgcgacttt
    13201 attcatcgga tactgccctt tctctttaat aaacgctgcg gctttatctg acggagaggt
    13261 ttcgaaacgg atatccagtt tcttatcgcc agctatcggt gctaaacgcc agttgttatc
    13321 tgccgccggg tgaatttccc ccgcacgttt cgactcatca gcaatccacg ctgccagcac
    13381 cgagcggttc tcatccggtg aagcaaaagc gatatggctg tcgcccgtac cggcaaattt
    13441 gccgccgtaa gcgcgatagt tattggtggc aacgaggaac atggcgttcg gatcaatcgg
    13501 cttgccgtta aaggtcaggt tcttaatcct ttccgcattg gcattaatca tctggcactc
    13561 gccgtcataa cgggcgggct gggtaacatc aatctgataa ttcacaccat caataacatc
    13621 aaagttatag gtgcggaaac catcccagtt gatgagtgac tgtggtttcg tgctgttagg
    13681 atcaatctgg ttaaactgtc cggcggagca ctccagccac tctttcacct ctttaccgct
    13741 ggctttcacc acaatcagcg tattggggta gagataaaga tcggcggcat tacggaaggt
    13801 caactggcct ttttccacct ccacatagct tgccgggtca tttttgcgac caccaacttt
    13861 aaacggtgcg gcagctgaaa gcactggcag ttttgccaga tccggatcgc cctgagtgta
    13921 atgctcgaca tacgcttttt gcgcgttgtt caccacctgc acggtcggat cgtcctgcac
    13981 aagcgccaga tagctataca tattgtcggc agatttaccg attggcttgc tgacgaactg
    14041 gcgtgtggca tcgtgatcgg ctttgagtgt ttctaccagc ttgctgtctt ccgccgcgag
    14101 ggatttttta ttagcgatgt cgtaaatcgg ccgtgcttcc gctttcgcct gcgtcacctg
    14161 ccatttaccg ctgtcattac tgagttgtaa gtcgaccaca ccaagatgat cgccccacat
    14221 gcctggcatt accgccggaa caccattcag cgtgcctttc gcgatatcag ccccttcgat
    14281 atcagcaaaa tctttacctg ggaaaacggc gtgagcatgg ccaaacataa tggcgttaac
    14341 gcccggaatt tcactgaggt aataaactga gttttccgcc atcactttat acggatcggc
    14401 agacagcccg gaatgcgcca gaacgacaac gacatcggca cctttctcgc gcatttcagg
    14461 cacgtatttg cgcacggttt cggtaatatc attcaccgtc actttcccgg ataaattagc
    14521 tttatcccag cccatgattt gtggcgggac gacgccaata taaccaatct tcagcgtctg
    14581 tttttttccg tctttatcga ccacttcggt gtctttaatt aaatacggtg taaacattgg
    14641 ctgtttggtt ctggcgtcaa tgacgttggc atttacataa gggaatttcg ctcccgccag
    14701 cgcatttttc agataatcca gaccgtagtt aaactcgtgg ttgccaagcg tgccgacggt
    14761 atagtccagc gtatttaatg ccttatagac cgggtgaata tcacctgctt ttaatccttt
    14821 cgccgacatg taatctgcca gcggactccc ctgaatcaaa tcgccgttat cgaccagtac
    14881 gctgtttttc acttcattgc gggcatcgtt aatcaggctt gccgtacgta ccagtccgaa
    14941 tttttccgtg gcggtgtctt tgtaataatc gaaatccatc atgttgctat gcagatcagt
    15001 ggtttccatg atacgcagat cgaccgtcgc tgcattcaca ctggcggcaa tcagcgtggc
    15061 caggagcgtt gcgctaaact taatcatcag ggacatcctt ttatcatcgg gaatacgaaa
    15121 gaaaagggag aataaacgtc ttacttatag aacagtgaag aatgccacaa ttttacgctt
    15181 tgaaaatgat gacactatca cagttggcgc attcattaac gatagggtat aagtaaaaca
    15241 ataagttaac accgctcaca gagacgaggt ggagaaatgt tagatcaagt atgccagctt
    15301 gcacggaatg caggcgatgc cattatgcag gtctacgacg ggacgaaacc gatggacgtc
    15361 gtcagcaaag cggacaattc tccggtaacg gcagcggata ttgccgctca caccgttatc
    15421 atggacggtt tacgtacgtt gacaccggat attccggtcc tttctgaaga agatcctccc
    15481 ggttgggaag tccgtcagca ctggcagtgt tactggctgg tagacccgct ggatggtact
    15541 aaagagttta ttaaacgtaa tggcgaattc accgttaaca ttgcgctcat cgaccacggt
    15601 aaaccgattt taggtgtggt gtatgcgccg gtaatgaacg taatgtacag cgcggcagaa
    15661 ggcaaagcct ggaaagaaga gtgcggtgtg cgcaagcaga ttcaggtccg cgatgcgcgc
    15721 ccgccgctgg tggtgatcag ccgttcccat gcagatgcgg agctgaaaga gtatctgcaa
    15781 cagcttggcg aacatcagac cacgtccatc ggctcttcgc tgaaattctg cctggtggcg
    15841 gaaggacagg cgcagctgta cccgcgtttc gggccaacga atatttggga taccgccgct
    15901 ggtcatgctg tggctgcagc tgccggagcg cacgttcacg actggcaggg taaaccgctg
    15961 gattacactc cgcgcgagtc gttcctgaat ccggggttca gagtgtctat ttattaatta
    16021 atttatgcag caggtccatc acttgctgca cctcttcctg agtaagcgcc ccgtctttgg
    16081 cccattgcac gcgaccgtct ttatccagca ccactacagc ggaactttct tcatccagct
    16141 gccaggcacc gcgtgcgacg ccattgctat caacaataaa ctgcgaccag ggataaagct
    16201 ttttattact ctccagactg ctgcgcacaa acatccctga acccggaatc gcgtcgtcgg
    16261 tgttaacaat ggtggtggtc tggtaacgat cgtgcggtaa cttcgctgat ttaatcgctt
    16321 caatcagcgt cgcgtttttc tcttttgcag aggtgcgacc agcaatatgt tgcagtactc
    16381 gcacttttcc cactaactgc gcgctgttcc aggttttgta gctaaactga tctttatcaa
    16441 gcaccaactc gccacgatcg gtgatgccaa tcggcggcac tcgctgaccg gtttcgaact
    16501 gatgtgcgga agccatcatc ggcaacagca ggcaggtgag cgccagaatc ttgcttaggg
    16561 tcatggtgtt tccttcttat gatatgcagg tgatccgacc acttgggtct gagttttaat
    16621 catatgtgct atttatcgaa tttcccgcaa gtgtgatgcc agtttgcggt caagcgcaca
    16681 aatcatatga aaaatgaatg cttatactga agaccgcgct tcggtaaaaa gataattctg
    16741 aataattgta acctttaggt aaaaaaagtt atacgcggtg gaaacattgc ccggatagtc
    16801 tatagtcact aggcattaaa atttgcgcct cataatagtt gggctgattg tggcaccgca
    16861 aaggcgtaat actcagcagg agataacaat gaaaattttc caacggtaca acccacttca
    16921 ggtggcgaag tacgtaaaga tcctgttccg tggacggtta tacatcaagg acgttggcgc
    16981 ttttgaattt gacaagggta agattcttat cccaaaagtg aaggataagc tgcatttgtc
    17041 agtgatgtcc gaagttaacc gtcaggttat gcgtctgcaa acagagatgg cttaaccaaa
    17101 gtgctatgca gtaaaaagtg ctatgcagta ataagacggc tcctgattca ggagccgttg
    17161 atgtttctgg ggttatgcga cgctttcttc tttatcttta gcgtcaggca gttttggcga
    17221 aagggcggtg gctttgctgt cgattcgggt caccagcagc tggtcgatgc ggtagttatc
    17281 gatatccacc acttcaaatt tgtagccggc gaatttcacc gaatcggtgc gtttcgggat
    17341 cttacgcagc ataaacatca taaagccgcc gatggtttcg tagttgcccg actgcgggaa
    17401 ctcgtcaata tccagcacgc gcatgacgtc gtcaatcgga gtgccgccat ctatcagcca
    17461 tgagttctca tcacgggcga caatctgctc ttccagcccc tgaccgacca gatcgcccat
    17521 cagcgtggtc atcacgtcgt tgagggtgat gatccccact accagcgcgt actcgttcat
    17581 gatcaccgcg aagtcttcac ctgcggtttt aaaactttcc aacgcctctg aaagggttaa
    17641 cgtatccggc acaatcagcg tattgcgaat ttgtacgccg ctgttcagtg ccaggctttg
    17701 gttagccagc acgcggttca gcaggtcttt agaatcgaca tagccgatga tgtggtcaat
    17761 atcttcatta cagacgagaa acttagagtg cggatgttcc gccaccttat tcttcagact
    17821 ttgctcatct tcgtggagat caaaccaaat cacgttttca cgcggtgtca ttgaagacgg
    17881 aacggtacgg gattccagct caaagacgtt ttcaatcagc tcgtgttcct gtttacgtaa
    17941 cacgcccgcc agcgcaccgg cttccactac cgcgtagata tcatcagaag tgatgtcatc
    18001 tttacgtacc attggcagtt tgaaaatacg gaagattatg ttcgccagtc cgttgaagaa
    18061 ccacaccagc ggggtgcaaa cgtacaggca gaagcgcatc gggttgatga tacgcaaagc
    18121 cacagcttct ggcgcaatca taccgatgcg tttcggggtt aaatccgcaa acaggataaa
    18181 catgccagtc actaacgaga aagagagaat aaagctcagt tgctcagaga gttctgccga
    18241 catatagcgg gagaacaggc tgtgaaaagc tggagaaaat gccgcatcac cgacgatacc
    18301 gccgagaatc gccactgcgt tcagaccgat ctggaccacg gtaaagaaca tgccgggatt
    18361 ttcctgcata ttcaaaacgc gttgggcatt tatattgcct tcatcagcca gcagtttaag
    18421 tttgattttg cgtgaggcgg caagcgagat ctcggacatc gagaagaacg cacttacagc
    18481 gatcaaacag agtatgacta aaatactgtt taacatatct tatccgacct ttcaggccag
    18541 atcctcggaa ggggaagttg attaatttgt gtgaaataca cattgaaagc cgattcgaag
    18601 aaagtgaacc ggcattttca gtggctagta tagcgtaagg tactgtaaag ccgccagagg
    18661 gttaaaattc agacagctga aaaatgaaaa actgcctgat gcgctatgcc gaacaggcct
    18721 acgcagtccc cgcaatatat tgaatttgga cgattttgta ggcctggcaa ggcgtttacg
    18781 ccgtatccgg cataaacaaa atgcacttta tcaacaatct acatttgtac ccgtaacgct
    18841 atgcttccgg cggcagacag acgccaattc cgccaattcc acagtaacca tacgggtttt
    18901 tatgcagata ttgctggtgg tcatcttcgg cataataaaa cggtgtggcg ttagcgattt
    18961 ccgtggtgat gcgacgatcg tcatcggcgg caagcatcgc cgcctgaaaa cgttccagac
    19021 tggcgcgagc ggcggcatcc tgttctgggg tcagcggata aatcgctgaa cgatactgcg
    19081 tgccgtggtc attgccctga cgcattccct gggcgggatc gtgactctcc caaaatactt
    19141 gtagcaactg ctcatagctg atgacggatg gatcgtaaac aatgcgcacc gcttcggcat
    19201 ggccagtatc gccggagcac acttcccgat aagtcggatt tggcgtatag ccgccggtat
    19261 agcctgcggc ggtgctgtaa acgccgggta actgccagaa cagacgctcc acaccccaaa
    19321 aacaacccat cgcaaaaatg gcaatctcca ttccgtcagg tacattggtc attgagtgac
    19381 cgttgaccgc atgcagtgtg gctacgggca tcggggtgtt acgtccaggc agggcatcgg
    19441 cgggggaaac cagatgcttt ttatcaaata aactcatggt gtcgctctcc cgaaaattgg
    19501 tcattggggt taaggttgta acaagaggcg tatttgcaca caataaccac cgtgaatagg
    19561 tctaaagtaa aacataagaa atattttggg tttagtctgc tttttaatcc atattactgg
    19621 atttttgtta agccgtttaa cggcgttcca ggggcaggaa aaaaggatat tcaggagaaa
    19681 atgtgcgcta tatccgacag ttatgctgtg taagcttact ctgcttaagc ggatctgccg
    19741 tcgccgcgaa cgtccgtcta caggtcgagg ggttatcggg agagctggaa aagaacgttc
    19801 gcgcgcagct ttctacgatt gaaagtgatg aagtgacgcc agaccgtcgc tttcgcgcac
    19861 gcgtcgatga tgccatccgc gaaggtctga aagcgctggg ctattaccag ccgaccattg
    19921 aatttgatct ccgtccaccg ccaaagaaag ggcggcaggt attgatcgcc aaagtcacgc
    19981 caggcgtgcc ggtgttaatt ggcggcaccg atgtggtatt gcgcggcggc gcgcggaccg
    20041 ataaagacta tttgaaattg ctcgatactc gcccggctat tggtacggta ctgaaccagg
    20101 gcgattatga aaatttcaaa aagtccttaa ccagcattgc gttgcgtaaa ggttatttcg
    20161 atagcgaatt taccaaagcg cagctgggca ttgcgctcgg cctgcataaa gccttctggg
    20221 atattgatta taacagtggc gaacgttacc gctttgggca tgtgaccttt gaaggatcac
    20281 aaatccgcga tgaatacctg caaaatctgg tgccgtttaa agagggcgat gagtacgaat
    20341 cgaaagatct ggcagaactg aaccgtcgac tttctgctac cggctggttt aactcggtgg
    20401 tggtggctcc acaatttgat aaagcgcgcg aaacgaaagt attaccattg acgggcgtgg
    20461 tttcgccgcg aacagaaaac accatcgaaa ccggcgtcgg ttactctacg gacgtgggac
    20521 cgcgcgtgaa agcgacgtgg aaaaagccgt ggatgaactc ttacggtcac agtttgacca
    20581 ccagtaccag tatttccgcg ccggaacaga tcctcgactt cagctataaa atgccgctgc
    20641 tgaagaatcc gctggaacaa tattatttgg tgcagggcgg ttttaagcgc actgacctga
    20701 acgataccga gtctgactcc actacgctgg tggcttctcg ctactgggat ctctccagcg
    20761 gctggcagcg tgccattaac ctgcgctgga gtttcgacca ctttacccag ggtgaaatta
    20821 ccaacaccac gatgctgttt tatcctgggg tgatgattag ccgcacgcgt tctcgtggtg
    20881 gcctgatgcc aacctggggc gactcgcaac gctactctat cgactactcc aacacggcct
    20941 ggggctcgga tgttgatttc tccgttttcc aggcgcagaa cgtctggatc cgcacactgt
    21001 acgatcgcca tcgttttgtg acacgcggca cgctgggctg gattgaaacc ggtgatttcg
    21061 acaaagtacc gccggatctg cgtttcttcg ccgggggcga tcgcagtatt cgcggctaca
    21121 aatacaaatc tatcgccccg aaatacgcca acggtgacct gaaaggggcc tcgaagctga
    21181 taaccggatc gctggaatac cagtacaacg tgaccggaaa atggtggggc gcggtgtttg
    21241 tcgatagcgg cgaagcggta agcgatattc gccgcagcga ctttaaaacc ggtaccggtg
    21301 tcggcgtgcg ctgggaatcg ccggtcgggc caatcaaact cgattttgcc gtgccggtcg
    21361 cggataaaga cgaacacggg ttacagtttt acatcggtct ggggccagaa ttatgagttt
    21421 atggaaaaaa atcagcctcg gcgtggttat cgttatctta ctgttgctgg gatcggtggc
    21481 gtttctggtg ggcaccacca gcggcctgca tctggtattt aaagcggcgg atcgctgggt
    21541 gccaggactg gatattggca aggtcaccgg cggctggcgt gatctcacct tgtctgacgt
    21601 tcgttatgag cagccaggcg tggcggtaaa agcgggcaat ctgcatctgg cggtcgggct
    21661 tgagtgcctg tggaacagta gcgtttgtat taatgatctg gcgctgaaag acattcaggt
    21721 caacatcgac agtaaaaaaa tgcctccttc tgaacaggtt gaagaagagg aagatagcgg
    21781 tccgctggat ctctccacgc cgtatcccat taccctgaca cgggtggcgc tggacaacgt
    21841 caacgtcaag attgatgaca ccacggtatc ggtgatggac ttcacctccg gcctgaactg
    21901 gcaggagaaa accctgaccc tgaaaccgac atcgctgaaa ggcctgttga ttgctttgcc
    21961 gaaagtggcg gaagcggcgc aggaagaagt ggtcgaaccg aagattgaaa atccgcagcc
    22021 ggaggaaaag ccgctcggcg aaacgctgaa agatctcttt tctcgcccgg tattgccgga
    22081 aatgaccgat gtgcatttgc cgcttaacct gaacattgaa gagtttaagg gcgagcagct
    22141 gcgcgtgacg ggcgacacgg acatcaccgt gagcaccatg ctgctgaaag tgagcagcat
    22201 tgacggcaat actaaactgg acgccctgga tatcgactcc agtcaaggga tcgtcaacgc
    22261 cagcggcacg gcgcagctgt cagacaactg gccggtggat atcaccctca acagcacact
    22321 gaacgtggag ccgttgaaag gtgaaaaggt gaagctgaaa gtgggcggcg cgctgcgcga
    22381 acagctggag ataggcgtta acctttccgg tccggtggat atggatttac gcgcccagac
    22441 gcgactggcg gaagccggat tgccgctcaa cgtggaagtg aacagcaaac agctttactg
    22501 gccgttcact ggtgagaagc agtatcaggc ggatgatctg aaactgaaac ttaccggcaa
    22561 aatgaccgat tacgcgatct ctatgcgcac ggctgttaag ggccaggaga tcccgccagc
    22621 caccattacc cttgatgcca aaggtaatga acagcaggtc aatctcgaca aacttaccgt
    22681 cgcggcgctg gaagggaaaa cggaacttaa ggcgttgctc gactggcagc aggccattag
    22741 ttggcgcggt gagctaacgc ttaacggcat taacaccgcc aaagagatcc cggagtggcc
    22801 gtcgaaactc aatggcttga ttaaaacccg cggtagcctg tacggcggca cctggcagat
    22861 ggaggtgcca gaactgaagc tgaccggtaa cgtcaaacag aacaaagtga acgttgacgg
    22921 cacgctgaaa ggcaacagtt atatgcagtg gatgatccca gggctgcatc tggaactggg
    22981 gccaaacagt gccgaagtga aaggcgagct gggggtaaaa gatctcaatc ttgatgccac
    23041 catcaacgcg ccggggctgg ataacgcgct gccggggctt ggcggtaccg cgaaagggct
    23101 ggtgaaagta cgcggcacgg tggaagcgcc acaactactg gcagatatca ccgcgcgcgg
    23161 cctgcgctgg caggaacttt ccgtggcgca ggttcgcgtg gaaggcgaca ttaaatccac
    23221 cgatcagatc gccgggaaac tcgacgtacg cgttgagcaa atttcgcagc cggatgtaaa
    23281 tatcaacctc gtcaccctga atgccaaagg cagtgaaaag cagcacgagc tacagttgcg
    23341 gattcagggc gagcctgtct ccgggcagct taatctggca ggaagttttg atcgcaaaga
    23401 agaacgctgg aagggaactc ttagcaatac ccgcttccag acgccggtcg gcccgtggtc
    23461 gctgacccgc gatattgcgc tggattaccg caataaggag caaaaaatca gcatcgggcc
    23521 acactgctgg cttaacccga atgcggaact gtgcgtgccg caaactatcg atgcgggtgc
    23581 cgaagggcgt gcggtggtga atctcaaccg cttcgacctg gccatgctga aaccatttat
    23641 gccagaaacc actcaggcca gcggtatctt cacgggtaaa gcggacgttg cctgggacac
    23701 cacgaaagag gggctgccgc agggcagtat cactctttcg gggcgtaatg tgcaggtgac
    23761 gcaaaccgtc aacgatgcgg cgctgcccgt ggcgtttcag acgctgaatc tgacggcgga
    23821 gttgcgtaac aaccgtgccg aattgggctg gaccatccgc ctgaccaata acggccagtt
    23881 tgatggtcag gtgcaggtga ccgatccgca aggccgccgt aatcttggtg gcaacgtcaa
    23941 tatccgtaac ttcaaccttg cgatgataaa ccccatcttt acccgtgggg aaaaagcagc
    24001 ggggatggtg agtgccaact tgcgtctggg tggtgatgtg caaagtccgc agttgtttgg
    24061 tcagcttcag gttacggggg tggatatcga cggcaacttt atgccgtttg atatgcaacc
    24121 gagccagctt gcggtcaact ttaacggtat gcgctcgacg cttgcaggta ccgtgcggac
    24181 ccagcagggt gaaatctacc tgaacggtga tgccgactgg agccagattg aaaactggcg
    24241 ggcgcgggta acagcgaagg gcagtaaagt gcgtatcacc gtgccgccga tggtacgaat
    24301 ggatgtatcg ccagatgttg tattcgaggc tacaccaaac ctgtttaccc tcgatggtcg
    24361 cgtggatgtt ccgtgggcgc gcatcgtggt gcacgatctg ccggaaagcg cagtaggcgt
    24421 ttccagcgat gtggtgatgc ttaacgataa cctgcaaccg gaagagccga aaacggcgtc
    24481 gattccgatt aacagtaacc tgactgtcca cgttggcgac aatgtgcgca ttgacgcctt
    24541 tggcctgaaa gcgcggctga cgggcgatct caacgtcgtt caggacaaac aagggctggg
    24601 cctgaacggg cagatcaaca tccctgaagg gcgcttccat gcctatggtc aggatctgat
    24661 tgtgcgtaag ggtgagctac tgttctctgg tccgccagat caaccgtatc ttaatatcga
    24721 agctattcgt aacccggatg ctacagaaga cgacgtcatc gccggagttc gcgtcactgg
    24781 tctggcggac gaaccgaaag cggagatctt ctctgacccg gcgatgtcgc aacaagctgc
    24841 attgtcttat ttgctacgtg gacaagggct ggagagcgat cagagcgaca gtgcggcaat
    24901 gacctcgatg ctgattggcc tgggggttgc gcaaagtggc cagattgtgg gtaaaatcgg
    24961 cgagacgttt ggcgtaagca atttagcgct cgacaccgag ggagttggcg actcctccca
    25021 ggtggtggtc agcggctatg tattgccagg cctgcaagtg aaatacggcg tgggtatatt
    25081 tgactctata gcaacactca cgttacgtta tcgcctgatg cctaagctat atctggaagc
    25141 cgtgtctggt gtagaccagg cactggattt gctctatcag ttcgagtttt agcaatgcga
    25201 atatttgtct acggcagttt acgccacaaa caaggcaaca gtcactggat gaccaatgcc
    25261 cagttactgg gcgatttcag tattgataac taccagttgt atagcctggg ccactatcca
    25321 ggcgcagttc cggggaacgg aacagtacat ggtgaagttt atcgtattga caacgccacg
    25381 ctggccgaac ttgatgcctt gcgcaccagg ggcggtgaat acgcgcgcca gttgattcag
    25441 acgccgtacg ggagtgcatg gatgtacgtt tatcaacgac ccgtcgatgg attaaagcta
    25501 attgaaagcg gcgactggtt agacagggat aagtaataat aaaagcacgc caccttcggg
    25561 tggcgttgtt ttatccacca gtctgaatat attccgaatt tgacgccatt cttatctttt
    25621 cttaaatgct gatttgttgc gcagtgttat aaacacctgg cacgctcaac aaggatgcga
    25681 ccacaatgaa aaaatctctt ctggctgtaa tgctgacagg actgtttgct ctcgtttctc
    25741 ttcccgctct gggaaatgtc aatctcgaac aattaaagca aaaagctgaa agtggagaag
    25801 ctaaagcaca gttggagctg ggatatcgct attttcaggg taatgaaacg acaaaggatc
    25861 tcaccctggc gatggactgg tttcgccgtg ccgcagagca gggatacacc ccggcagaat
    25921 atgtactggg gttacgctat atgaacggtg aaggtgtgcc gcaagattat gctcaggcag
    25981 ttatctggta caaaaaagcg gcactgaagg ggcttccgca agcgcagcag aatctgggtg
    26041 taatgtacca tgaaggtaat ggcgtgaagg ttgataaagc cgaatctgtg aaatggtttc
    26101 gcctggcagc agagcaaggt cgtgacagcg gccagcaaag tatgggagac gcatattttg
    26161 aaggcgatgg tgtgacgcgg gattacgtta tggcacgtga gtggtatagc aaagcagcgg
    26221 aacaaggtaa cgtctggtcc tgtaaccagc ttggttatat gtattccaga ggtttaggcg
    26281 ttgaaagaaa cgatgccata tcggcacaat ggtatcgaaa atcagcgaca tcaggcgacg
    26341 agttggggca gcttcattta gccgatatgt actatttcgg tatcggtgtt actcaggatt
    26401 acactcagtc acgggtatta ttttcccagt cggcagagca gggaaattct attgcgcagt
    26461 ttcgtctggg atatatattg gagcaaggtt tagcgggagc gaaagagccg ttaaaagcac
    26521 tggagtggta ccgtaaatcg gcagaacagg gaaattccga tggtcagtat tatttggcac
    26581 atctgtatga taaaggcgca gaaggtgtag caaaaaatcg agaacaagcc atctcctggt
    26641 acacgaaatc ggcagaacag ggggatgcta ccgcacaggc taatctcggc gctatttact
    26701 tcagacttgg ttcagaagaa gaacataaaa aggcggtgga atggtttcgc aaggccgccg
    26761 caaagggtga gaaagcggcg caatttaatt tgggtaatgc tttactccag ggaaaaggtg
    26821 ttaaaaaaga tgagcaacag gccgcaatct ggatgcgaaa agccgcagag caaggattaa
    26881 gtgcagcgca ggtacaatta ggtgaaatct attattatgg cttgggcgta gaacgtgatt
    26941 atgtgcaggc ctgggcgtgg ttcgataccg catcgaccaa tgatatgaat ctttttggta
    27001 cagaaaaccg caacattaca gagaaaaaac tgacagccaa acaactgcaa caggctgaat
    27061 tattatcgca acaatatata gaaaaatatg ccccggaagc ctgggcgaga atgcaaaagc
    27121 ttaaagcgca atcagcggta aagacgggta ataaataacg ggtaactgtc agatcaggtc
    27181 tgcgctaata cagctcaacc aatgccacct gcgggtggcg ttattttttt gcgagacgac
    27241 gcgtattctt ttttgtaact tcctcgcctt ttgcttttcc ttccgagccg ctttccatat
    27301 ccgttaacgc atgtaaaact ctgctggcgt taacaaatgt gcaggggtaa aacgggggca
    27361 cgctttgaac ggtgagtatg agtgaggccg gctaacttcc agcaagtggc tatgaacagc
    27421 aaaatggtga tcgagtttag cttgttctta ccgttcaagc ctttaaactg ctgaaaataa
    27481 taatagtgga gtagagagtt tataaattgc gggcataaaa aaacccctac taaccgaagc
    27541 ccggcgttca gggttattac gccagaagaa cttatttatt cttggcgcgc tcgaaggaag
    27601 caacgatttc agctttagcg gcttctgcgt tttcccaacc ttcaacttta acccacttgc
    27661 ctttttcgag gtctttgtag tgctcgaaga agtgagcgat ttgcgctttc agcagctctg
    27721 gcagatcgtt aacgtcttta atgtgatcgt attctttgct cagcttggtg tgcggaacag
    27781 caatcagttt cgcatcttca ccggcttcgt cggtcatttt cagtacgcca accggacggc
    27841 agcggatcac agaacccggc tgcagcgggt acggagtcgg gaccagtacg tcaaccgggt
    27901 caccgtccag agacagggtg tggttgatgt aaccgtagtt gcacggatag aacatcgcgg
    27961 tggacatgaa gcggtcaacg aacagtgcgc cgctctcttt gtcgatttcg tatttgatcg
    28021 gatctgcgtt agccgggatc tcaataacaa cgtagatgtc ttccggcaga tctttacccg
    28081 cagggacgtt gagtaagctc atgtctgttt cctttaaaaa tatgtggcaa acaagtgccg
    28141 agtattatag ccaactcgcg ccgaatgtct tcgcttgttt tcgtcttcat tttccttttt
    28201 cgccagtttt aagacggtat acagagcagg aaaatccata accgtagccg catttttcat
    28261 agtgagatga aagcgattac aaacttgtga ttaacgtttt atttactttt ttgaagtgtg
    28321 atgtaacgca atccgttaca taacgaattg tctatagttt tttcgcgaac atcttttcac
    28381 caataataat taccccgacg aggacaaccc tatgtggaaa cgcttacttg tagtctctgc
    28441 agtctcggca gccatgtcgt ctatggcgtt ggccgctcca ttaaccgtag gattttcgca
    28501 ggtcggatcg gaatccggct ggcgcgccgc agaaaccaat gtggcgaaaa gtgaagccga
    28561 aaaacgcggt atcacactga aaattgccga tggtcagcaa aagcaggaaa accagattaa
    28621 agcggtacgt tccttcgtcg cacaaggggt ggatgcgatc tttatcgctc cggtggtggc
    28681 gacgggttgg gaaccggtat taaaagaggc gaaagatgcc gaaatcccgg tcttcttgct
    28741 tgaccgttcc atcgatgtga aagacaaatc tctctatatg accactgtca ccgccgacaa
    28801 catcctcgaa ggcaagttga ttggtgactg gctggtaaaa gaagtgaatg gcaaaccatg
    28861 caacgtggtg gagctgcagg gcaccgttgg ggccagcgtc gccattgacc gtaagaaagg
    28921 ctttgccgaa gccattaaga atgcgccaaa tatcaaaatt atccgctcgc agtcaggtga
    28981 cttcacccgc agcaaaggca aagaagtgat ggagagcttt atcaaagcgg aaaacaacgg
    29041 caaaaacatc tgcatggttt acgcccataa cgatgacatg gtgattggtg caattcaggc
    29101 aattaaagaa gcgggcctga aaccgggcaa agatatcctg acaggctcta tcgacggcgt
    29161 gccggacatc tacaaagcga tgattgatgg cgaagcgaac gccagcgttg aactgacgcc
    29221 gaacatggca ggccccgctt ttgatgcgct ggagaaatat aaaaaagacg gcaccatgcc
    29281 tgaaaagcta accctgacca aatccaccct ttatctgcct gataccgcaa aagaagagtt
    29341 agagaagaag aaaaatatgg ggtattgagg gttgctatgc ctgatgccga ttcgtaggcc
    29401 ggataaggcg ctcgcgccgc atccggcggt ggtgcgctga agcctgatgc gacgcttacc
    29461 gcgtcttatc atgcctactg ggagcacgct ttacaccggg ggaaaccatg acgaccgacc
    29521 aacaccagga aatcctccgc accgaaggat taagtaaatt tttccccggt gtcaaagcgt
    29581 tagacaacgt tgatttcagc ctgcgccgtg gtgaaatcat ggcgctgctc ggtgaaaacg
    29641 gggcgggaaa atcgacgctc atcaaagcat tagccggtgt ataccacgcc gatcgcggca
    29701 ctatctggct ggaaggccag gctatctcac cgaaaaacac cgcccacgca caacaactcg
    29761 gcatcggcac cgtctatcag gaagtcaacc tgctacccaa tatgtcggtc gctgataatc
    29821 tattcatagg tcgcgaaccc aaacgtttcg gccttctacg ccgtaaagag atagaaaagc
    29881 gcgccaccga actgatggca tcttacggtt tctccctcga cgtgcgcgaa ccgctcaacc
    29941 gcttttcagt cgcgatgcag caaatcgtcg ccatttgccg ggctatcgat ctctccgcca
    30001 aagtgctgat cctcgatgaa cccaccgcca gtctcgacac tcaggaagta gagttactgt
    30061 ttggcctgat gcgtcagctt cgcgatcgcg gcgtcagtct gatcttcgtc actcactttc
    30121 tcgatcaggt ctatcaggtc agcgatcgga tcaccgtctt acgtaacggc agttttgtgg
    30181 gctgtcggga aacccgcgag ctaccgcaga tcgaactggt aaaaatgatg ctggggcgtg
    30241 aactggacac ccacgcgctt cagcgtgccg ggagaacatt gctgagcgac aaacctgttg
    30301 ccgcgttcaa aaattacggc aaaaaaggaa cgatcgcgcc gtttgatctc gaagtacgcc
    30361 ccggcgagat cgtcggtctg gcaggcttgc tgggatcagg acgtaccgaa accgccgaag
    30421 tgatcttcgg tatcaaacct gctgatagcg gcacggcgtt gatcaaaggc aaactgcaaa
    30481 cgctacgctc gccacatcag gcttcggtac tgggcattgg cttctgcccg gaagacagga
    30541 aaaccgatgg cattatcgcc gccgcctcgg tgcgggaaaa tatcatcctc gctctccagg
    30601 cccagcgcgg ctggctacga cctatttccc gtaaagaaca gcaagagatt gccgaacgct
    30661 ttatccgcca gcttggcatt cgcacacctt ccaccgaaca accgattgaa tttctctccg
    30721 gcggcaatca gcaaaaagtg ttgctttcac gttggctact gacccgaccg caatttctga
    30781 ttctcgacga gccaacgcgc ggaattgatg ttggtgccca caccgagatc atccgcctga
    30841 ttgaaacgct gtgcgccgat gggctggcgc tgctggtgat ctcctccgag ctggaagagc
    30901 tggtgggcta tgccgaccgg gtgattatca tgcgcgatcg caaacaggta gcggagatcc
    30961 cgctggcaga gctttccgtt ccggcgatca tgaacgccat tgcggcgtaa ggagaatagt
    31021 gtgatgcctc aatctctccc ggacactacg ccgccgaaaa ggcgttttcg ctggccaaca
    31081 ggaatgccgc agctggcggc actgttgctg gtgctgctgg tcgatagcct ggtggccccg
    31141 catttctggc aggtggtgct tcaggatggg cgtttgttcg gtagccctat cgacattctt
    31201 aaccgtgcgg cccccgttgc gctactggcg attggcatga cgctggttat cgccaccggt
    31261 gggattgatc tctccgtagg ggcggtgatg gctatcgccg gagccacaac ggctgcgatg
    31321 acggtcgcgg gattcagcct gccaattgtt ttgttaagcg ccctgggcac tggcatcctg
    31381 gcaggattgt ggaacggcat actggtagcg atcctcaaaa ttcagccgtt tgtcgctacc
    31441 ctgatcctga tggtcgccgg gcgcggcgtg gcgcaactga tcacctccgg acagatcgtc
    31501 acatttaact caccggatct ctcctggttc ggcagtggat cgctgttgtt cctgccaacg
    31561 ccagtcatta ttgcggtgct gacgcttctc ctgttctggc tgttgacccg caaaacggca
    31621 ctggggatgt ttatcgaagc cgttggtatc aacattcggg cggcaaaaaa tgccggggta
    31681 aacacgcgga tcatcgtcat gctcacctac gtgttgagcg ggctatgtgc ggctattgcg
    31741 ggcattatcg tggcggcgga tattcgcggt gccgatgcca ataacgccgg gttatggctg
    31801 gagctggacg ccattctcgc agtggtgatt ggcggcggat cgctgatggg cgggcgcttt
    31861 aacctgctgc tttcggtggt gggggcgctg attattcagg gaatgaacac cggaattttg
    31921 ctttctggct ttccgccgga gatgaaccag gtggtgaaag cggtggtggt gctttgcgtg
    31981 ctgattgttc agtcgcaacg ctttatcagt ctgattaaag gggtacgtaa ccgtgataaa
    32041 acgtaatttg ccactgatga tcaccatcgg cgtctttgtg ttgggttata tctactgtct
    32101 gacccagttt cccggttttg cttccacaag agtgatctgc aatatcctga ccgataatgc
    32161 ctttcttggg ataatcgccg ttggcatgac ctttgtgatc ctctccggtg ggatcgatct
    32221 ctccgtcggt tcggtgatcg cctttaccgg cgtgtttctg gcaaaagtga ttggcgattt
    32281 cggcctctcg ccgctgctgg cgtttccgct ggtgttggtg atgggctgtg ccttcggcgc
    32341 atttatgggg cttctgattg atgccctgaa gatcccggcg tttatcatta cccttgcggg
    32401 aatgttcttt ttgcgtggcg tcagctatct cgtttcggaa gagtcgatcc cgataaacca
    32461 tcctgtttat gacacgctct caagccttgc gtggaaaatc cctggcggtg gtcgcttaag
    32521 tacgatggga ctactgatgc tggcggtggt ggttatcggc atattactcg cgcatcgtac
    32581 ccgttttggt aatcaggtat acgccattgg cggcaacgca acgtcggcaa acctgatggg
    32641 gatttccact cgcagcacca ctattcgcat ttatatgctc tccaccggac tggcaacgct
    32701 ggcggggatt gtcttctcga tttataccca ggccggatat gcactggcgg gcgtaggtgt
    32761 ggaactggat gctatcgcct cagtggtaat tggcggtacg cttttgagtg gtggcgttgg
    32821 aacggtatta gggacgcttt ttggcgtggc gattcaggga ctgattcaga cttacataaa
    32881 ctttgatggc acgctgagtt cctggtggac gaaaatcgcc atcggtattt tgttgtttat
    32941 ttttatagca ttacagcgtg gattaacggt gctgtgggag aatcgtcaga gttcgccagt
    33001 gacaagagtc aacattgcgc agcgataaaa acgcctctcc gtgtggagag gcgcaggagc
    33061 ttacgcgtcc gggaactcac ggataaagcg ttcgacatct tcaaccatat ggtcgttgcc
    33121 gacaaagaac gaacggcgct ggtgcagggt ttccgggata atatccagaa tacgctcttt
    33181 gccatcgctc gctttaccgc ccgcttgttc cgccaggaat gccatcgggt tgcactcata
    33241 cagtaaacgt agtttaccgt ccgggtggct ggcggtgctt gggtagagat aaataccgcc
    33301 tttcagcagg ttacggtgga aatccgcgac cagtgaaccg atataacgtg aggtatacgg
    33361 gcggttggtg gatttatctt cttcctggca gaatttaatg tacttcttca ccccgttcgg
    33421 aaacttaatg tagtttcctt cgttgatgga gtaggttttg cctttctccg ggaagcgcat
    33481 ccgttcctgg cacaggcaga aaacacccag cgaaggatca taagtaaagg cgtgaacacc
    33541 gcatccggtg gtgtaaacca gcatagtaga ggagccgtat accacgtaac ctgccgcaac
    33601 ctgtttgtta ccaggctgga ggaaatcttc ttccgttacc ggcgtgccaa caggcgtaac
    33661 gcggcggtag atagagaaaa tggtaccgac agagacgtta acatcgatgt tggacgagcc
    33721 atccagtggg tccatcagca ccacgtattt tgcgtgttca cagccttcaa agacgacaat
    33781 ctcatcttct tcttcagagg caatgcccgc aacgatatcg cgtgctttca gtgcggcttt
    33841 cagtttctca ttagcgaaca ggtcgagttt ctgctgaacc tcgccctgca cgttctcagc
    33901 accgctggca cccaggatat caaccagtcc tgctttgttg atatcgcgat ggataatctt
    33961 ggcgcccagt tttattgccg acagcaaagc agtgagctca ccggtagcat gagaaaactc
    34021 gtgctgcttt tcgacaataa attcacctaa cgttttcata aaactttccc tgcaatgttt
    34081 atggagtaaa gcgaccgcaa caatcttaac aaataatccc aatgttgcgc tcaggtgaat
    34141 cgcgccagca aattacggat tatcctgaaa tgcgtttctc acttgcccga catatgcgta
    34201 aaatgagcgc cagattaaaa aaggatagtg acgtatgcgc attcatattt taggaatttg
    34261 tggcacgttt atgggcggtc tggcgatgct ggcgcgccag ttaggccatg aagtaacggg
    34321 ttcggacgcc aatgtgtatc cgccgatgag caccttactt gagaagcaag gcattgaact
    34381 gattcagggt tacgatgcca gccagctcga tccacagccg gatctggtga ttattggtaa
    34441 cgccatgacc cgtggaaatc cgtgtgtgga agcggtactg gaaaaaaaca tcccttatat
    34501 gtcaggtccg cagtggctgc acgattttgt gctgcgcgac cgctgggtgc tggccgttgc
    34561 cggtacacac ggcaaaacca ccacggcggg aatggcgacc tggattctgg aacaatgcgg
    34621 ttacaaaccg ggatttgtga tcggcggtgt gccggggaac tttgaggttt cggcgcgtct
    34681 gggcgaaagc gacttctttg ttatcgaagc ggatgagtac gactgcgcct tcttcgacaa
    34741 acgctctaaa tttgtccatt actgcccgcg tacgctgatc ctcaacaacc ttgagttcga
    34801 tcacgccgat atctttgacg acctgaaagc gatccagaaa cagttccacc atctggtgcg
    34861 tatcgttccg gggcagggcc gtattatctg gccggaaaac gatatcaacc tgaaacagac
    34921 catggcgatg ggctgttgga gcgagcagga gctggtgggc gagcaaggtc actggcaggc
    34981 gaaaaagctg accactgacg cttctgaatg ggaagtcttg ctggatggcg aaaaagtggg
    35041 cgaagtgaaa tggtcgctgg taggcgaaca taatatgcac aatggcctga tggcgattgc
    35101 ggcggctcgc catgttggtg tagcgccggc agatgccgct aacgcgctgg gttcgtttat
    35161 taacgctcgt cgccgtctgg agttgcgtgg tgaagcgaat ggcgtcacgg tatatgacga
    35221 ttttgcccat cacccgacgg cgattctggc aacgcttgcg gcgctgcgtg gcaaagttgg
    35281 tggtacggcg cgcattattg ccgtgctgga accgcgctca aacaccatga aaatggggat
    35341 ctgcaaagac gatctggcac cttcattagg tcgtgccgat gaagtcttcc tgctgcaacc
    35401 ggcacatatt ccgtggcagg tggcagaagt ggcagaagcc tgcgttcagc ctgcacactg
    35461 gagcggcgat gtggatacgc tggcagatat ggtggtgaaa accgctcagc ctggcgacca
    35521 tattctggtg atgagcaacg gcggttttgg tgggatccat cagaaactgc tggatgggct
    35581 ggcgaagaaa gcggaagctg cgcagtaatt cacagcctca gcctgataga gtgtgtgtag
    35641 gccggataag gcgttcacgc cgcatccggc atttgagcat agtgcctgat gcgacgcttg
    35701 atgcgtctta tcaggcctac aggtgcaccg tatccggcaa accatactca catcaacaac
    35761 gaaaattacc cttcgttttc cgctaactca cgcagatact ggaaaatctg gcgcgcggat
    35821 ttcggcggct tattcccttc tttctctttc ttcgcgttgc ggatcagagt tcgcagttgc
    35881 tgacgatcgg catcgggcca cagattcaat acttcagcga tggcgtcatc accctggtcg
    35941 atcagacgat cgcgcaggtt ttccagttta tggaacagca ccacctgctg gttgtgacgg
    36001 tttttcagct tatccagcgc ctgacggatc ggttctacgt cgcgctggcg cagcatctta
    36061 ccgatgagct gcaactggcg gcggcgacct tccatcttaa tacgctgggc gagctcaata
    36121 gccgcgcgca gatccgcatc taacgggatc ttatccagcg cgtttttccc cagatcaaca
    36181 atttccgcgc caaggcgttt tagctcctcg gcatcacgtt taatttcact tttactgacc
    36241 cagataattt catcgtcttc gtcttcgatg tcatcaccgg gaacgtcgtc gagccagtct
    36301 tcgggctgct tagtcatctc aggctcctta aaaaaagagg ctaatgttac cagttaagat
    36361 gcgcactgaa aaacggttct ctgttagact tcagagaaac tctctacatt atggcacttg
    36421 caatgaaagt aatctctcaa gttgaagcgc agcgcaagat tctggaagaa gcagtttcga
    36481 ctgcgctgga gttggcctca ggcaaatcgg acggtgcgga agttgccgtc agcaagacca
    36541 ccggcattag cgtaagcacg cgttatggtg aagtggagaa tgttgaattc aatagcgatg
    36601 gcgcgctggg gatcaccgtt tatcaccaga accgtaaagg tagcgcatcg tctaccgatt
    36661 taagcccgca ggccattgcc cgcaccgtac aggcggcgct ggatattgcc cgttatacct
    36721 cgccagatcc gtatgcaggc gtggcagaca aggaactgct ggctttcgac gcgccggatc
    36781 ttgacctgtt ccaccctgcg gaagtttccc cggatgaagc cattgaactg gcggcccgcg
    36841 ctgaacaggc ggcattgcag gcggataaac gcatcaccaa taccgaaggt ggcagcttta
    36901 acagccacta cggtgtcaaa gtttttggca acagccacgg catgttgcag ggttactgct
    36961 caacgcgtca ttcgctctcc agctgtgtaa tcgccgaaga aaatggcgat atggagcgtg
    37021 attacgccta caccattggg cgtgcgatga gcgatctgca aacgccagag tgggtcgggg
    37081 ccgactgtgc tcgccgtacc ttatcgcgtc tgtcaccgcg taaactttct accatgaaag
    37141 cgccggtgat ttttgccaat gaagtggcaa cagggttgtt tggtcatttg gtgggggcga
    37201 tagcgggtgg tgcggtctac cgtaaatcga cctttttgct cgactcgctc ggtaaacaaa
    37261 ttctgccgga ctggctgact attgaagaac atccgcatct gctgaaaggg ctggcatcta
    37321 caccattcga cagcgaaggt gtgcgcaccg agcgccgcga tatcgtcaaa gatggcatcc
    37381 tgactcagtg gctgctgacc agctattcgg cgcggaaact ggggctgaaa agcaccggac
    37441 atgcgggcgg tattcacaac tggcggattg ccggacaagg gctaagcttc gaacaaatgc
    37501 tcaaagagat gggcaccggg ctggtggtga cggagctgat gggccagggc gtgagtgcca
    37561 ttaccggtga ttattcccgt ggtgcagcgg gcttctgggt agagaatggc gagattcagt
    37621 atccggtgag cgaaatcacc atcgctggta atttaaaaga tatgtggcgc aatattgtca
    37681 ccgtcggtaa cgatattgaa acacgcagta atatacagtg tggttctgtg ctgttgccgg
    37741 agatgaaaat cgccgggcag taaaaaactg gcgcgacctg ccgcgctata aactaaaaaa
    37801 ttacacaaaa tcattcgcac tgcatcgaga cggaaagtga atgagagccg gggagcgtac
    37861 tcgtagtacg tgactcgggc gaatgagcgt agccgtcgat gaggcagtgt gaaggatgaa
    37921 gtgtaaataa aaaaggaagt gagcaatgcg taaaagcctg ttagctattc ttgcagtctc
    37981 ctcattggta ttcagttcgg cgtcgtttgc cgctgatctg gaagacaata tggaaaccct
    38041 caacgacaat ttaaaagtgg tcgaaaaagc cgataacgcg gtgcaagtca aagacgcgtt
    38101 aacgaagatg cgcgctgcgg cgctggatgc gcaaaaagcg acgccgccga agcttgaagg
    38161 caaatcaccg gacagcccgg aaatgaaaga cttccgccac ggtttcgaca ttctggtcgg
    38221 tcagattgac gacgcgctga agctggcaaa tgaaggtaaa gtaaaagaag cgcaggctgc
    38281 cgcagagcaa ctgaaaacga cccgcaacgc ctatcaccag aagtatcgtt aattctttat
    38341 ttccctgttg cctgcactca ggtaacaggg aacttatcat cgcaaatgat gcaccacctg
    38401 attgctgctg ccgcgccaga tcagggaagg gtcttttaaa tcctgcacaa atttgccgtc
    38461 gaccagcacg ttaatcaaat caacaacctg catttgcgca gcgttgagtt cgtcgagttt
    38521 atagcctgtc cacacccaga tgtctttacc cggacactcg gcgcggatgc gttgtaccag
    38581 tttcagaata tccggcacgt tttgcggatg cagcggatcg ccgccggaga gggaaatacc
    38641 ctggcgtttg atgcgagtgt cattcagatc gttaatgatc tggtcttcca ttgctttggt
    38701 aaatggctga ccggaattta cccgccaggt gcttttgtta tagcaaccgg ggcattcatg
    38761 aacacacccg gagacaaaca gagtgcagcg agtgccgggg ccgttgacga tgtcgacagg
    38821 atagtactga tgatagttca ttatttcacc tggcttatca gtccgcgtgt aggctgcatc
    38881 cggtaatcaa cgcctgatgc gacgctgtct cgtcttatca ggcctacagc cgttgccaaa
    38941 tgtaggccgg ataaggcgtt tacgccgcat ccggcatttg cttaacacag agtgaagatt
    39001 aacctatctg cccatttccc aaatgtttaa cgcggcgctt aacttcttcc tgcttaccag
    39061 cgttaaacgg acgtgcatcc gggctaccta aatatccgca cacgcggcga gtcaccgaca
    39121 cacgggaggt gtcatggtta ccacatttcg gacaagtgaa gcctttgctg gtgcactcaa
    39181 attcgccggt aaagccacac tcgtagcatt catcgatcgg tgtgttggtg ccgtaatacg
    39241 gtacatgctg atagctgtaa tcccagacat cttccagcgc cttcaggttg tgctgaatgt
    39301 ttggatactc gccatagcaa atgaagccac cgttcgccag tggcgggtaa ggcgcttcaa
    39361 agtcgatctt gtcgtacggg ttcaccttct tctccacatc gaggtggaaa ctgttggtgt
    39421 agtaaccttt atcggtcacg cccggcacca cgccaaactc agcggtatcg agacggcaga
    39481 agcggtcgca caggttttca ctcggcgtgc tgtagagact gaaaccataa cccgtttctt
    39541 ctttccactg atccactgcc tgacgcagac gttcaacaat cgcgatacct ttcgcgcgca
    39601 gctgctcgtt gtcgtaaaca tgctcgccgc cgaacagcgc gttaatggtt tcgtggatgc
    39661 cgatgtaacc cagcgaaata gacgcacgac cgtttttgca gatttcagaa acatcatcgt
    39721 cagcattcag acgcacgcca caagcacctt ccatatagag gatcggggcc acgcgcgctt
    39781 tcacgccttc gagacgagcg atacgggtca tcagcgcctt acgtgccagc accagacgtt
    39841 catccagcag cttccagaag gtggcttcat cgccttttgc ttccagagca atacgcggca
    39901 ggttcaggct gatcacgccg aggttgttac gaccatcgtg gatctgctcg ccgttttcat
    39961 tttcccacac gccgaggaag ctgcggcagc ccatcggggt tttaaacgaa cctgtgactt
    40021 tcactacctg atcgtagttc aggatatccg gatacatgcg cttgcttgcg cactccagcg
    40081 ccagctgttt gatgtcgtag ttcggatcgc cttttttatg gttcaggcca tcgcgaatcg
    40141 caaacaccag tttcgggaac accgccgttt tacggttttt accaaggcct gcgatacggt
    40201 tacgcaggat agattcctga atcaggcgcg attcccagct ggtgcccaga ccaaaaccaa
    40261 aggtcacaaa cggtgtttga ccgttggcgg tgtggagggt gtttacttcg tactccagcg
    40321 actggaaggc atcgtagcac tctttgatgg tacgagagtt agcgtagcct tcggcgtccg
    40381 ggatgttcca ctcttctgcg gtcttgcgat gtttgttgta gctggcggtg acaaacggtg
    40441 ccagcacttc atcgatacgg ttaatggtgg tgccgccata aatatggctg gcgacctgag
    40501 caataatctg cgcagttacc gcggttgccg tagagatcga tttcggcggt tcaatctcgg
    40561 cgttccccat tttaaagccc tgggtcagca tgcctttcag gtcgatcaac atgcagttga
    40621 acatcgggaa gaacggtgag taatcgagat cgtgatagtg aatatcacca cgttcatgtg
    40681 cctgcaccac gtcacgcggc agcaggtgct ggcgtgcata gtgtttagcc acgatcccgg
    40741 ccagcaggtc gcgctgggtt ggaatcacct tgctgtcttt gttggcgttt tcgttgagta
    40801 acgaggagtt ggtctgctcg accagaccac ggatctcctg gttcaggcga ccgcgttttt
    40861 cacgttcaat gtcgcgatcg tgacggtact cgatgtaagc acgagccagt tgtttgtatg
    40921 gccccgacat cagctggttt tcaactgcgg tctggatctc attgatatcc acctggttgc
    40981 ggccctgcat ctgctcgctg acaaccgcag caacagtggc gcaataatcg gcatcatcga
    41041 cttccgctgc tttagctgca cgcagaatcg cttctttgat gcgctctgat ttaaacggca
    41101 ctttgcagcc gtctcgtttc atcacatgcg gtgtcatgat cgctccatat ttttaagaac
    41161 aggttatcca cagaaattgg gaaaggcgtt ttccggtttt ttcgtttcgt tttccggtgc
    41221 tttccgcaat cgccatccgc ttatccacaa cgtcgcacct ttttcgtggg gagctgtagt
    41281 agcaattata gtcgattaat acaacatatt gggttgggac gcattttaaa gtctatatat
    41341 agtgctttgc atcaaggatg tttgaacttt ttttgatgta gctcaaagta aaaagcagag
    41401 cgtacggatg acgggcgcta cagcgatatg taaatttttt aatgaatttt ttgattataa
    41461 aatcaacaaa aacaacatac tgacaggcaa aaccccggaa tgacaggcat taccggggct
    41521 tagcgaagat ttacttctgt aaccaccaga cagcctcaaa aggccgtaaa gtcatggtac
    41581 agggttgtgg tgaggcttct tcgtagttat gcatcacaag ctgccagttg ccgagcattt
    41641 tccctggctg ccagggttgg gtctcacggc taaggttggc aatgaccagc aaggtttgcc
    41701 ccttccactc acggcgatag caccacaata cagggctgtt tggcagcaga tcctggtaat
    41761 cgccccatgt caggacggct tcttgcttac gcagtgcgat taacttttgg taggtgtaaa
    41821 acaccgagga ctcatcggcc agcgctgctt ctacgttgat ttcctgatag ttatcgccca
    41881 ggccaatcca cggttcgcca gccgtaaacc cggcattatc gccgttggtc cattgcatgg
    41941 gtgtgcgact gttgtcacgg gatttactgg cgaggattgc caataactcg tcggcatcac
    42001 gcccatcgtt gcgcagctcg gcaaacatat tgaggctctc cacgtcgcga tagtcagtaa
    42061 tgcgcgtgaa atgtgggttg gtcatgccaa tctcttcgcc ctggtagata tacgacgttc
    42121 cctgcatgcc atgcagcacc atcgccagca tttttgccgc aggcacgcgg tattcacctt
    42181 catcaccaaa gcgagaaaca atgcgcggct gatcgtggtt acaccagaac aaggcattcc
    42241 atgccacgtt gtgcattcct tgttgccagt ggcggaacaa tgttttcaac gctacaaagt
    42301 caggtttagc cagcgtccat ttttcacctc cgggataatc gaccttcagg tgatgaaaat
    42361 taaaggtcat cgacaattca ctgcctgtca gtgccgcgta tcgctggcaa tgctcaaggc
    42421 tggtggagga catttcacct acggtcatta acccgcgtgg cgtaaacaca tcgcggttca
    42481 tctcgtgcaa aaactcgtgt gctcgtggcc cgtcggtata gaagcgacgc ccgtcgccat
    42541 ccaggtcttc agggtaacgc ggatctttgg agatcagatt caccacatcc aggcgcaacc
    42601 cgtcgacccc acgatcggcc cagaactcac agactttttt caactctgcg cgtaccgctg
    42661 gattctccca gttgagatcc gcctgttctg gtgcaaagag atgcaaatag tactgttcgc
    42721 tttctgcatg ccagcgccac gcactaccac cgaattttga acgccagttg ttcggtggcg
    42781 tttctggttc tccatcgcgc cagatataaa actggcggta agggctttct ttgttcagcg
    42841 cctcgcgaaa ccaggcatgt tgggtagagg tatggttaaa caccatatcg agaatgatac
    42901 gaatcccacg cgattttgcc tgtgtcacca gttcgtcaaa atcgtccagc gtgccatagg
    42961 tgggatcaat cgccgtataa ttcgctacgt cgtaaccgtt atcgacctgg ggagagacat
    43021 aaaagggggt tagccagatg gcatcaacgc ccagtttatg cagatagtcc aggcgttgga
    43081 taacgccacg taaatcgccg gtgccgctac ccgtggtgtc ctgaaaactc tttggataaa
    43141 tctggtagat aacgccgttt tgccaccagt ggggaagatg agtcattacg ttattcctgc
    43201 aaatgcgagg gggcgcaatt gcgccccgaa gaaaattaaa caatgtccag cgtgcccagg
    43261 cggtatttcc gctgatagat aaacgaggtg agtacaatcg ggatgatgat ggcgatagcc
    43321 attgccagcg caaacacctg ccagtagctc ggctgaatcg agagaattcc cggcaggccg
    43381 cctacgccga tgccattcgc cataacgccg ttcaggccgc atagcaatcc tgccagacca
    43441 gaaccaatca tcgcgcacag catcgggaag cgatatttca ggttgatgcc gtacatagca
    43501 ggctcagtga ctccaagcca ggcggagata gcggcaggca cggagatctc gcgttcattg
    43561 tgcttgcggc tggaaatgat aatacctatc acggcggagc cctgagcaat attcgacagc
    43621 gcaatcagcg gccacactgg cgtgccaccc atgctttgaa tcatctgcaa atcaatagca
    43681 agcgtggtct ggtgtacgcc ggtgatcacc agcggggcgt acaggaagcc aaacaatgcc
    43741 gcgccaatcg gagcaaagct gccggtcatc aggtgacgta ccgcaaaggc aacgccatcg
    43801 ccaatcatgc gaccaaacgg accaatcagc gcatgggcga ggaacaccgc gaggatcagc
    43861 gaacagacgg gcaccaccac cagatagagg taatccggca cgatgcgctt aagacgagtt
    43921 tcaataacgc ccagcgccag tccggctaac agtgccggga tgacctgcgc ctgatagccc
    43981 actttggcga tgctgaatat gccaaagtcc catacttccg gcagctgctg cccgagcaga
    44041 taagcgttca tcagctgtgg agaaaccagc gttacgccaa gcacgatacc gaggatcggt
    44101 gtaccaccca tttttttcac cgctgaccag caaatcccga ccggcaggta gaagaagatc
    44161 gcttcaccga tcaaccacag aaaatcgtag atcgtttgca gggaagggta catttgcgcc
    44221 agcgtctgac cgttgctcat gggcaaatcg ccgatcacat tgcgaaaacc gaggatcaaa
    44281 ccaccactaa tcaacgcggg cagcaatggg aagaagatct ccgcgaaatg agagatcaac
    44341 tgctcatgcc atttcatatt ctgccgggcg gcttttttta cctgctcttt atcaacctgc
    44401 gcctgtccgg ttgacgcaat cagtgcttga tagtaatcac ccacgttggt gccaatcacc
    44461 acctgaaatt gcccggcatt ggtgaaacag cctttcacca tggggagttg ctcaatttct
    44521 ttcggtctgg cattggccgg ttggttgagg acaaagcgca ggcgagtaat acagtggctc
    44581 accgtcgcaa tattgccgcg cccgccgacc atttcaatca accgatcgat atccgtttgg
    44641 tttattttgc tcatcataaa gccccatggc agatgacatt tttggttggc tgcagaatat
    44701 attgcgcgga aaaatttaaa aacgggaacg ttcccgaaac acagcgaaga tcacaattta
    44761 tcgttcggga aacgatcagg acagggtggc ggggatgatg atttgttgcg gttcgctgcg
    44821 cccggtgacc tgcgcgatca actggcaggc cgcctggcgt ccagcttcgg cgtaaccggg
    44881 atctacggtg acgatctccg gatggaggaa tttcattaac ggtgtattac cgacgctcgc
    44941 cagttgcaag gtgtcgatgc gttgctcttg caggtattta cttgcgccaa gtgccagcgt
    45001 gtcggttgcg cacagtaacg cggtagtttc aggcgtaatc acttttgcta cgttctcata
    45061 gccttgcttc atagcaagcc ctggcagagc ggcaacggga tgcagtttat gcgctttgca
    45121 gaacgccagg taggcttcgt gacgtcgctt accggtcgtc acgtcactgt gcggcacgcc
    45181 gagatagctg atattacgat gtccctggtc atacagccgt tgcatcagga tcttgattgc
    45241 cccttcgtcg tcataacaga ccgaagcaaa gccttttgcg tcacgcgcca gcagaaccag
    45301 cgatgactgc cagtgggcta acatttcttc tgttatgccg gtaaaaccga acagcactac
    45361 gccgtcgata ttacgccgtt tcagcacccc caaatgttcg gcaactaatt gtggggaaaa
    45421 ctgactttcc atcatgattg gatcgtaacc ttgttcatag aacgctggca gcatggtttg
    45481 aacggcgaga ttttctgaca acgaatccag acgggtaaca atgatggcga ccactttatc
    45541 gctttgccca cgcatagcgc gcgcagagcg ggaaggggaa aatccatgct gattcatcac
    45601 tgcttcaaca cgctcgcgcg tgcgctggct cacgccgctt tcgttattca gcacccggga
    45661 aaccgtagat ttccccacgc cgcttaagcg cgcgatgtct ttgatggtca gccgattttg
    45721 catcctgttg tcccataacg tgttgtttaa ttatttgagc ctaactttac ctgtgcattc
    45781 agcaatgggt aaagtctggt ttatcgttgg tttagttgcc agcaggtatt atatcgccat
    45841 aaatgctacg aatattattg gattctcctt attatttgcg gcgctttttt cacttaccgg
    45901 aggttatatg gaacctgatc ccacgcctct ccctcgacgg agattaaaac ttttccggta
    45961 agcccgtctt ttcacggcgt taccggatgc gtaaggccgt gacgttttaa cgtccctgct
    46021 cagctttatt accttcaggt aaggcttcgc cacgcctgaa gacatttctg tactgtttca
    46081 gacagtgcgg agggactcct tatgtttaaa gaaattttta cccggctcat tcgccattta
    46141 ccttcccgtc tggttcatcg tgatccattg cctggcgcgc agcagacagt gaatgcggcg
    46201 gtcccgcctt ctttaagtgc gcattgcctg aaaatggcgg tgatgcccga agaagaattg
    46261 tggaaaacgt tcgacaccca tccggaaggg ttaaatctgg cggaagtgga atctgcccgc
    46321 gaacaacatg gtgaaaataa attacccgca caacaaccgt cgccgtggtg ggtacattta
    46381 tgggtctgct atcgcaaccc ctttaatatt ttgctcacca ttcttggtgc tatttcttac
    46441 gccacggaag atttatttgc cgcaggtgtt atcgcgctaa tggtcgctat ttctacgttg
    46501 ctgaacttta ttcaggaagc tcgttccacc aaagcggcag atgccctgaa agcgatggtc
    46561 agtaataccg cgacggtact gcgagtaatt aacgacaaag gcgaaaatgg ctggctggag
    46621 atcccgatag accagctggt gcccggcgat attataaaac tggcggcggg ggatatgatc
    46681 ccggcagatt tacgtatctt gcaggcgcgg gatctgtttg tcgctcaggc gtcgttaacc
    46741 ggtgagtctc tgcccgtaga aaaagccgct accactcgcc agccggagca cagcaatccg
    46801 ctggagtgcg acaccctgtg ttttatgggc accaccgtgg tgagcggcac ggcacaagca
    46861 atggtgattg ctacaggagc caacacctgg tttggtcaac tggcggggcg tgttagtgag
    46921 caggaaagcg agccgaatgc ctttcagcaa gggattagcc gcgtcagtat gctgctgatt
    46981 cgctttatgc tggtgatggc tccggtggtg ttgcttatca acggttacac caaaggtgac
    47041 tggtgggaag cggcactgtt tgcgctttcg gtcgcggtag gcttaacgcc agaaatgttg
    47101 ccgatgattg ttacctcgac gctggcgcgt ggcgcagtca agttgtcgaa acagaaagtg
    47161 atcgtcaaac atctggatgc cattcagaac tttggcgcaa tggatattct gtgcactgat
    47221 aaaaccggca ccctgacaca ggataaaatt gtgctggaga atcataccga tatctccggt
    47281 aaaactagcg aacgcgtgct gcatagcgcg tggttgaaca gccattacca gaccgggctt
    47341 aaaaacctgc ttgatacagc ggtgctcgaa ggtacggatg aagagtcagc gcgctcgctg
    47401 gccagccgtt ggcaaaaaat tgatgagatt ccgtttgatt tcgagcgtcg tcggatgtcg
    47461 gtggttgtgg cagaaaatac cgaacaccat cagttggttt gcaaaggcgc attgcaggaa
    47521 atcctcaatg tgtgttcgca agtgcgtcac aatggcgaga ttgtaccgct cgatgacacc
    47581 atgctgcgta agattaagcg ggttactgat acgctgaatc gtcaggggct gcgcgtggtt
    47641 gcggtggcga cgaaatacct gccagcgcgt gaaggagatt accagcgggc ggatgaatcc
    47701 gacctgatcc tcgaaggata tattgctttc cttgatccgc caaaagagac aacagctccg
    47761 gcactgaagg cattaaaagc gagtggaatt accgtaaaaa tccttactgg cgacagtgag
    47821 ttagttgcag cgaaagtgtg ccatgaagtg gggctggacg cgggagaggt ggtcattggt
    47881 agtgatattg aaacgctatc tgacgacgaa ctggcaaatc tcgcacagcg caccacgctg
    47941 tttgctcgtc tgacgccgat gcataaagaa cgcatcgtaa cgttgctgaa gcgcgaaggg
    48001 catgtggttg gctttatggg cgatggtatt aatgatgcgc ccgccttacg cgctgcggat
    48061 atcggcattt ctgtggacgg cgcggtagat attgcccgtg aagcggctga tatcatccta
    48121 ctggaaaaaa gcctgatggt gctggaagag ggagttattg agggacgtcg cactttcgcc
    48181 aacatgctga aatacatcaa aatgacggcg agctctaact tcggtaatgt gttcagcgtg
    48241 ctggtggcga gtgctttctt gcccttcctg ccgatgttgc cgctacactt gcttattcaa
    48301 aatctgctgt acgatgtgtc acaggtggcg atcccatttg ataacgtcga cgacgagcaa
    48361 attcaaaagc cgcagcgttg gaatccggcg gatctggggc gctttatggt cttcttcgga
    48421 ccgatcagct cgattttcga tattctgacg ttttgcctga tgtggtgggt attccatgcc
    48481 aacacgccgg aaacgcaaac gctgttccag tcgggctggt ttgtggtggg cttactgtcg
    48541 caaacgctga ttgtgcatat gatccgcacc cgccgtgtgc cgtttattca gagctgtgca
    48601 tcgtggccgt taatgatcat gaccgtgatc gtgatgattg tcgggatcgc attgccgttt
    48661 tcaccgctgg ccagttatct gcaactgcag gcgctgccgt taagctattt cccgtggctg
    48721 gttgcgattc tggcagggta tatgacgtta acccagttgg tgaaaggttt ctatagccgt
    48781 cgttacggtt ggcagtaaag aataaactgg gcatggtggc ccggaatcag attgcagaca
    48841 aagtgagctt tgttcatgcc ggatgcggcg taaccgccta atccggtcta caaattcaat
    48901 aaattgcagg aatcacgtag gcctgataag cgtagcgcat caggcgattt tgcttttgtc
    48961 atcagactta atccgggcat gatagcccgg atttccatca agattagcga cgaacagcga
    49021 tcgcttcgat ctcaatcttc acgtctttcg gcagacgggc tacttcaacg caagaacgtg
    49081 ccgggaaggt ggcgttgtgt tcggtgaaga aggcttcgta ggtagcatta acggttgcga
    49141 agtcgttcag atctttcaca aacacggtgg ttttaacgat atcgcccact ttcaggccag
    49201 cggcttcgac gatcgctttt acgttatcca gcgactgacg tgcctgtgca gcgacatctg
    49261 ccgggacttc gcccgttttc gggtttaccg ggatctgacc ggaagtgata atcatattgc
    49321 ccagatcaac gccctgtacg taaggaccga tagctgccgg tgcattttcc gtcgcgatag
    49381 ttttgctcat gatttctcct ttattacagc ggtaaaaagt ctggctcatt atagggagcc
    49441 agacttttat taccaaccgc aattaattgg ccagcaccac attatgagaa aactcttttt
    49501 cacagtattt gcatttgagc gcgatatcat tggcgcgttt tcgcacggca aagctggatg
    49561 aaaccggttc ggcatggctg atacagttgc tgttagggca gaccagcaca ttgtcgatgc
    49621 gctctggcaa actggggcgc gatttgccaa ccacttcata gttgtcgata cggttaaccg
    49681 tggcttgcgg tgcatataac gccagctgat caacctgatc ctcacttaaa aaggtatttt
    49741 cgattttgat caaatctttg cggcccatct cgccagaagg caaattcaga ccaatggtga
    49801 tgcgctgatc tgtttcggtc aacttgaaca gactcaacag cttaaaaccg atctgggcgg
    49861 ggatatggtc aattaccgtg ccgcgtttaa tagcttcaac ctgcaattta ttatcgtgtg
    49921 tcatctctat ttccccttac agtaccagat cgcgattcag aaccagtgcc agtaacgcct
    49981 ggcgagcgaa aatcccgttg cctgcttgct ggaagtacca ggcgtgaggc gttttatcca
    50041 catccgtcgc aatctcatca acacgcggca gcggatgcag cactttcata ttggctttgg
    50101 cgttgtggag atcgctggcg cgaagaacaa actgcgcttt cacgttggcg tactcggacg
    50161 ggtccagacg ctctttttgc acgcgggtca tgtacaggat gtctacttcc gccatcactt
    50221 cttcaataga gctgtgcaga ctccaggcga tccctttttc atcgagcata tccagaatgt
    50281 attgcggcat tgccagcgca tccggcgcga taaagtaaaa acggttgccg tcgaacttcg
    50341 ctagcgcctg ggtcagggag tgaacggtgc ggccatattt caggtcacca accattgcga
    50401 cgtggagatt gtccagacgc ccctgggttt cctgaatggt gaataagtcc agcaaggttt
    50461 gcgtcggatg ttggttggag ccatcaccgg cattcagtac cggtacattg ccggaaaact
    50521 cggtggccag gcgcgctgca ccttcctgcg gatgacgcat cactatcgca tcgacgtaag
    50581 tgctgataac cgaaatggta tcggccagcg tttcgccctt tttacccagt gatgtattgg
    50641 cgctgtcgga gaagcccacc acgctggcac ccaggcggtg catggaagtt tcgaaagaga
    50701 ggcgggtgcg ggtagaggct tcgaagaaac agctggcaat aactttgtgc ttcaacagct
    50761 ctggttgcgg gttcgctttc agtttcgccg ctgtcgccag caccagatta aggtcatcgc
    50821 gactaaggtc gtttatggaa atgatatgtt tctgatatag cggattagcc atcttttatc
    50881 tcctgacgcc tgggcaaaaa aaagcccctc gattgagggg ctgggaatgg gtgatcaacg
    50941 ggaagaaaaa cggcaggcca gcgtcttttt tcagacgcgg taagacaaaa tgtcgaacac
    51001 actgaaccat acatcctccc ggcaaattgt ccggcattat actcagctct gagagaggat
    51061 caagaagatg atgcagttat aaagcaaggc aaacggtcat catgaatata tattcaattt
    51121 taataagtaa ttaatttgct tgtgcaccag ggctgtacgc tttaccacac gtggtgcgac
    51181 ccaaacgata ttgctgccag caatcacgcc aagaatttct ggcaaagcat ggaaatcaag
    51241 gattcgcgct actgcccgac catatccggc gacggtatgg ataaggataa attcaccatt
    51301 atgctcgaca ctcaggacca tttcagcgac ggatcgacct gcgtcaggtg tgggaagtaa
    51361 ttgaggattc accgaataaa ttttttgtcc ttttgtattt ctaattttta tgactcccag
    51421 caatttcagt agtcgtgaaa cggttgactg actgatactg tcaaagccat ggttttgcaa
    51481 atcgcggcgg atttcctcct gtgaaagata acttttttcg gtaatcaggc gctgacagac
    51541 cgccagttgc ttcttttctt tggcggagta atcatcgtat tccttcataa tgcacctttt
    51601 atttattatt acctgatgat atttttcaca ttttataatg tgagagtggt tcatttatca
    51661 ctacaccaga tttgaaggcc gctttgataa gcggcctgtc gcgttaaatc atcgcggcga
    51721 tactcagcac aatcatattc aacatcgtga gaatcagcag tagcggtgct acccatctca
    51781 gataacggac ccagggtaca cgggcaatcg ccagaccgcc catcactacc gcagaggttg
    51841 gcgtaatcaa attaaccacc ccggaagcag actgataagc ggtcaccacc agatcgcgtt
    51901 gtacatgagc aaaatcagcg agtggagcca atattggcat cgttaacact gcaaggccgg
    51961 aagatgaggg aaccagaaaa gagagcaata cttccagcca ataggtcacg ttgatgaaaa
    52021 cagtggtgga aagccctgaa accagattct cagcactgtg cagaattgtg tgcgtaatca
    52081 tgccgttatc catcacgacc acaataccgc gggcgatgcc gataatcagt gccacaccaa
    52141 ggagatcccg cgcgccgtca ataaaggtac tggtgaaggt ttcttcgccc atgcgggcaa
    52201 tgacacccac gatgaccgca gctgccagaa agacagcaga gatttccgcc atccaccaac
    52261 ccagtaccgc tacgccgtag atcataaagg caaaggatgc agcaaaaagt atcaggatcg
    52321 ctttgcgcgt gggggtaaat tccagcatcg tttctgaacg atttcccaga aaatgcgcct
    52381 ggttttcagc catcttatcc gccacgatag agctttccgg actgttgcga actttgcggg
    52441 cataacgcat aacccagtaa acacaaatca cgtagcctac taacagcaaa ataacacgca
    52501 gcaaaatgcc ctgagtaaag gggatccctg cggcgttggc agcaatgacg gtagcaaatg
    52561 ggttaatggt tgagcccagc gtgccaatgc ccgatcccaa taacactgtg gcggcagcga
    52621 ccagaggatc aaagcgggca gccatcatga cgggcaccag caacgtgtag aaggggagtg
    52681 attcttccgc catcccataa atagtaccgc cagcagcgaa gagcgccatc aggatcggaa
    52741 tcatccactc ttcccgacca ttaaggcgag ttgtcactcg ttcgataccg gcatctattg
    52801 ccccagtttt atttactact cccaaaaatc caccgatgat caggacaaac agcgcgacat
    52861 caatcgctcc cgcagtgtag gtttgatggt tgtacaagcc gtcgatgggc gctaacagga
    52921 cagccgtaat accctgtggt tgtgcctcaa caggtgcgta agtgcctgca acaggaactt
    52981 cctttcccag tgcggcattt gtcaccatct gatattttcc cgcaggtaca atccaggtca
    53041 ttgccgcaac taacgcaatc agcacgaaca agatggtata agcggtggga aatttgaatt
    53101 tacccataat gtgatctcca taatcacggg catacgccac tcgtcgacgc atgcccgtca
    53161 gggttagtcg ccgagagttg ctaccatcac cgctttaatg gtgtgcatcc ggttttctgc
    53221 ttcatcgaaa acaatagagt gggccgattc aaaaacctct tcagtaactt ccagtccttt
    53281 gaggccgtag gccatctcga tctcgcgtcc tactgtggtg tgctcattat ggaaggcggg
    53341 caggcagtgc ataaatttaa cttcagggtt acgcgtggca gtaatcacct gttgattgat
    53401 ctgataaggt gccatcagac tcactcgttc tgcccaggct tcttttggct ctcccatcga
    53461 aacccaaaca tccgtataga gaaaatcaac gtcgtatacg ccttcttcca catcctctgt
    53521 cagggtgatt ctcgccccag tcacgctggc gatctcacgg cagtgtgtta ccagcgcttc
    53581 atctggccag aaagattttg gtgccaccag acggatatcc atccccattt ttgcagcacc
    53641 aaccatcagc gagttcccca tgttatttcg ggcatcacca agatacgcaa agctcagctc
    53701 tggcagcgtt ttgcctggcg catgttccag catggtcatt aaatccgcga ggatctgtgt
    53761 cgggtgaaat tcattggtca gtccgttcca taccggaaca ccagcgaact cacctaattc
    53821 ttcaacgata ttctgaccat aaccgcgata ctcaataccg tcatacatgc gtccgagtac
    53881 gcgagcggta tctttcattg attctttatg gccgatttgt gagccgctgg ggccgatgta
    53941 agtcacctgc gcaccttgat caaaggccgc gacttcaaag gcacagcggg tacgggtaga
    54001 ggatttttca aaaatcaacg caatgttttt accgaccagc gtttgttttt cgtttccaga
    54061 tttcttggcg gctttaagat tgatagccaa atcaatcagg tattgaatct ctgctggggt
    54121 gtaatcaagc agcttaagaa aattacggtt tttcagtgaa gtagccatgg ggatgtcctt
    54181 ataaaaatgg atcaagcgtg gttatggggg gtatcaaggc gaataagagt gcctttgtct
    54241 ccggcgagaa tttccggacc atctgcaaga gagccgatcc ctgcaatgcc ccggcactga
    54301 ctaacaaact ttgcgcaggc ggtgactttc ggccccatag acccggcatc aaactgcatt
    54361 tcgttgagta attccggcgt gacctgggcc agcgggcgtt gggttggctt gccccagtcg
    54421 agatatactg cgtccgcatc agtgaggatc aacaaggcgt cagcatggat ctgacttgcc
    54481 agtagcgcgg ctgaaagatc tttgtcgata accgcttcaa tcccatggta tccatcggct
    54541 ttttcaacca ccggaacacc accgccacca ttacagatca ccagatgatc gtgggcgatc
    54601 agtgtctgga tggcatcccg ctcgacgatg cgtttaggtt gtggagaagg tacgacccgg
    54661 cggaaagagt gaccatctgc tttgaaaacc cagtcttttt ctgcctgtag tacctgagtc
    54721 tgagcgtggt cataaattgg gccgatgtac ttagttgggt tactgaaggc gggatcgttg
    54781 gcatccactt caacctgcgt cagcagcacg ctgatttcac gttgtggtag ttgatttttc
    54841 agcgcctgtt gtagcatgta gccgatcatc ccctggcttt cggcccccag aatgtcgagt
    54901 ggatagggag cgacatgggc ataggcgcta ttttgaagtg ccagaagtcc aacttgtggg
    54961 ccattcccgt gaaccagtac aacccgccag tgttgagtca gttgggcgat agttttagcc
    55021 gcaagatcga tatttttacg ttggatttct gcttcgagtg gttcgcctcg tttaagcagg
    55081 gcattaccgc cgagagcgat aaccagtgtt ggtttgtttt ccatgatctc ttccttagat
    55141 accgtcacga tgcaaagggc aactcatgca acgtgcgccg ccacgtccgc ggcctaattc
    55201 atcaccgggg atgggtaaaa cggtgatgcc tgctttgtca tatttttcgt tagtccagat
    55261 gttgcgctcg tagcccacta ccactcccgg ccgaagcgtg agcacattgt tagcgtcatt
    55321 ccactgttcg cgttctgcct caaaggcatc accccccgtg gtgattaaac gtacctggtc
    55381 aataccgagg gctttttcga tagcatggag gagtgtgctt tcttgagtac gtttcaggcc
    55441 gccgtgaccg tcaggtgtta gcgtccagca attaacatcc gggcgcacaa cttcgggata
    55501 aaccgagaag gtgtcgatat cgatatgagt cataacggtg tcaagatgca tacaggagcg
    55561 gtgtttaggc agctcaacag cgattacccg ttcagcctga cgatgtttaa acaatgcctg
    55621 agcaagaaac tcgattcctt gtggcgtggt gcgttcagac atgccgatca acacggcccc
    55681 ccgaccaatc accagcacat caccaccttc taacgtggcg tggtcataat taatattctc
    55741 gtcgccgaaa tatttaataa attcgccgcc agcaaattgt ggatgccagc gataaatagc
    55801 gcgtagatta ttggtttcac gttggcgagc aggttttgcc attggattaa tcgatacacc
    55861 gttataaatc cagcatgagg tatcacgcgt aaataaatga tttggtaacg gcttcataat
    55921 gaaatcatta atatcgtggg tatcaaccac catattttta attgaggcgg gaatttcact
    55981 ataagtcagc ccaccactta aatgacgagc aaggtcccgg tgtgacatct ctgcaagcca
    56041 ggtgcgaacg tcagtagcaa acgtagggcc gagtcggtaa tctgatattt gtgtttccag
    56101 taaccagctt ttggcttccg ggatatccaa tgtttgtgtt agtaaatcag tgagtaacag
    56161 aacttctatc ccttgctggc gcagcgtatt ggcgaaaata tcatgctctt cacctgcccg
    56221 ctcaacggac aatacatcgt caaataacaa ctcctggcaa ttggatggtg ttagacgttt
    56281 cagactgaga tttgggcgat gcaacataac gctgcgtagt tgtccaattt cagaaccaac
    56341 ataatgcttt tccatcatta ttcctttaac actttttcaa aaataaagag gagatggctg
    56401 caaacaaatt aaatgcttgt agtcaggtct gttcagtaat tttcagatgc aatgattcag
    56461 tttatttctt ccagaaaatg tgacgacttt cacaccggcg gtgatattaa taatcgaagt
    56521 tgataatatt tgatgcggtt ctgataaatg ataaagaaac aaaatacgat aatgcattaa
    56581 tacgcaaata tacgcagagt tattcttcgt tgttaattg
//
LOCUS       DAZGJT010000018        56529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZGJT010000018 DAZGJT010000000
VERSION     DAZGJT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 56529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.018"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            27..302
                     /gene="ypdI"
                     /locus_tag="ACHKFK_001210"
     CDS             27..302
                     /gene="ypdI"
                     /locus_tag="ACHKFK_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFO9051597.1"
                     /translation="MKVNLMLFSLFLFVSIMACNVFAFSISGGGSEASYKETEKTSVM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEDITPTYPTKANYLIHPLR"
     gene            complement(379..621)
                     /gene="yfdY"
                     /locus_tag="ACHKFK_001211"
     CDS             complement(379..621)
                     /gene="yfdY"
                     /locus_tag="ACHKFK_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO9051598.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNMVSAISSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            974..1894
                     /gene="lpxP"
                     /locus_tag="ACHKFK_001212"
     CDS             974..1894
                     /gene="lpxP"
                     /locus_tag="ACHKFK_001212"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO9051599.1"
                     /translation="MFPKCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GTMARPFLKRRESIARKNLELCFPQHSAKEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVSALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLYITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2250..2321
                     /gene="ypdK"
                     /locus_tag="ACHKFK_001213"
     CDS             2250..2321
                     /gene="ypdK"
                     /locus_tag="ACHKFK_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO9051600.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2386..3624)
                     /gene="alaC"
                     /locus_tag="ACHKFK_001214"
     CDS             complement(2386..3624)
                     /gene="alaC"
                     /locus_tag="ACHKFK_001214"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO9051601.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLYTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4001..5698
                     /gene="ypdA"
                     /locus_tag="ACHKFK_001215"
     CDS             4001..5698
                     /gene="ypdA"
                     /locus_tag="ACHKFK_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO9051602.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAMTNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5713..6447
                     /gene="ypdB"
                     /locus_tag="ACHKFK_001216"
     CDS             5713..6447
                     /gene="ypdB"
                     /locus_tag="ACHKFK_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO9051603.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6460..7317
                     /gene="ypdC"
                     /locus_tag="ACHKFK_001217"
     CDS             6460..7317
                     /gene="ypdC"
                     /locus_tag="ACHKFK_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9051604.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPNSLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPVNNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHDVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7320..9815)
                     /gene="ptsP"
                     /locus_tag="ACHKFK_001218"
     CDS             complement(7320..9815)
                     /gene="ptsP"
                     /locus_tag="ACHKFK_001218"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9051605.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTLAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRVIPASAQDSTLLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRHLMAEQHQGLGAAIISNMEQ
                     ICAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDKKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTAAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFAPEED
                     VRPLLALENIFVDQSFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLVKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9840..10877)
                     /gene="ypdE"
                     /locus_tag="ACHKFK_001219"
     CDS             complement(9840..10877)
                     /gene="ypdE"
                     /locus_tag="ACHKFK_001219"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9051606.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQSVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSCDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLIAWIETVAAEIGVPLQSD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10877..11962)
                     /gene="ypdF"
                     /locus_tag="ACHKFK_001220"
     CDS             complement(10877..11962)
                     /gene="ypdF"
                     /locus_tag="ACHKFK_001220"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO9051607.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYADVEARTQGYQLHLLDAMHTLATIVRQIIADEQLQTLGFEGQQVSW
                     ETAHRWQSELNAKLVSATPDVLRQIKTPEEVEKIRLACGIADRGAEHIRRFIQAGMSE
                     CEIAAELEWFMRRQGAEKASFDTIVASGWRGALPHGKASDKIVTAGEFVTLDFGALYR
                     GYCSDMTRTLLVNGEGVSAESHPLFNVYQIVLQAQLAAISAIRPGVRCQQVDDAARRV
                     ITEAGYGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11978..13225)
                     /gene="fryC"
                     /locus_tag="ACHKFK_001221"
     CDS             complement(11978..13225)
                     /gene="fryC"
                     /locus_tag="ACHKFK_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9051608.1"
                     /translation="MAIKKRSATVVHGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPADIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13247..13573)
                     /gene="fryB"
                     /locus_tag="ACHKFK_001222"
     CDS             complement(13247..13573)
                     /gene="fryB"
                     /locus_tag="ACHKFK_001222"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9051609.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(13792..14757)
                     /gene="glk"
                     /locus_tag="ACHKFK_001223"
     CDS             complement(13792..14757)
                     /gene="glk"
                     /locus_tag="ACHKFK_001223"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO9051610.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEGIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14961..16217
                     /gene="yfeO"
                     /locus_tag="ACHKFK_001224"
     CDS             14961..16217
                     /gene="yfeO"
                     /locus_tag="ACHKFK_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO9051611.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIMVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSNEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHEMMHQMKNPVFVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16332..16658
                     /gene="ypeC"
                     /locus_tag="ACHKFK_001225"
     CDS             16332..16658
                     /gene="ypeC"
                     /locus_tag="ACHKFK_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO9051612.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16799..18037)
                     /gene="mntH"
                     /locus_tag="ACHKFK_001226"
     CDS             complement(16799..18037)
                     /gene="mntH"
                     /locus_tag="ACHKFK_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO9051613.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGRGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDGKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18373..19575
                     /gene="nupC"
                     /locus_tag="ACHKFK_001227"
     CDS             18373..19575
                     /gene="nupC"
                     /locus_tag="ACHKFK_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO9051614.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19624..21813)
                     /gene="pdeA"
                     /locus_tag="ACHKFK_001228"
     CDS             complement(19624..21813)
                     /gene="pdeA"
                     /locus_tag="ACHKFK_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO9051615.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRLISPLAVIHSNYIFL
                     AWMPLCVMLSILFIFGWRGVVPILCGMFCTNLWNLHLTFLQTAVMIGSQAFAVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVAPIGIKCSMYLVGNFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLSKEKRAFTLS
                     WLAALSVLLLLMCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVLTEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLIMRVHCIRSIYRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGHFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRVSVNLMPLTLLQKNIAGRIIRLFKRYYISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(22020..22095)
                     /locus_tag="ACHKFK_001229"
     tRNA            complement(22020..22095)
                     /locus_tag="ACHKFK_001229"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22060..22062),aa:Ala,seq:ggc)
     gene            complement(22135..22210)
                     /locus_tag="ACHKFK_001230"
     tRNA            complement(22135..22210)
                     /locus_tag="ACHKFK_001230"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22175..22177),aa:Ala,seq:ggc)
     gene            22431..22790
                     /gene="yfeC"
                     /locus_tag="ACHKFK_001231"
     CDS             22431..22790
                     /gene="yfeC"
                     /locus_tag="ACHKFK_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9051616.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22792..23184
                     /gene="yfeD"
                     /locus_tag="ACHKFK_001232"
     CDS             22792..23184
                     /gene="yfeD"
                     /locus_tag="ACHKFK_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO9051617.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVETQVCEFIQNTPAFHNTPMLLEAEEPLAEYAPGIRTPAYRQIISAIDNMTH
                     SEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23235..24650)
                     /gene="gltX"
                     /locus_tag="ACHKFK_001233"
     CDS             complement(23235..24650)
                     /gene="gltX"
                     /locus_tag="ACHKFK_001233"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO9051618.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLTAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALAFIAERENQQ"
     gene            24909..24984
                     /locus_tag="ACHKFK_001234"
     tRNA            24909..24984
                     /locus_tag="ACHKFK_001234"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24942..24944,aa:Val,seq:tac)
     gene            25029..25104
                     /locus_tag="ACHKFK_001235"
     tRNA            25029..25104
                     /locus_tag="ACHKFK_001235"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25062..25064,aa:Val,seq:tac)
     gene            25151..25226
                     /locus_tag="ACHKFK_001236"
     tRNA            25151..25226
                     /locus_tag="ACHKFK_001236"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25184..25186,aa:Val,seq:tac)
     gene            25231..25306
                     /locus_tag="ACHKFK_001237"
     tRNA            25231..25306
                     /locus_tag="ACHKFK_001237"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25264..25266,aa:Lys,seq:ttt)
     gene            complement(25417..26301)
                     /gene="xapR"
                     /locus_tag="ACHKFK_001238"
     CDS             complement(25417..26301)
                     /gene="xapR"
                     /locus_tag="ACHKFK_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO9051619.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVVFRPLKERIPADLYIVY
                     ETQQVTPALVKLLAALAQ"
     gene            complement(26342..26500)
                     /locus_tag="ACHKFK_001239"
     CDS             complement(26342..26500)
                     /locus_tag="ACHKFK_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO9051620.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     LVVKYFMP"
     gene            complement(26553..27809)
                     /gene="xapB"
                     /locus_tag="ACHKFK_001240"
     CDS             complement(26553..27809)
                     /gene="xapB"
                     /locus_tag="ACHKFK_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO9051621.1"
                     /translation="MSIALRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQVGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSPTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWHTIWLMFAGYAL
                     FLAVIFFFGFKYNHDPEKITHRSVTH"
     gene            complement(27870..28703)
                     /gene="xapA"
                     /locus_tag="ACHKFK_001241"
     CDS             complement(27870..28703)
                     /gene="xapA"
                     /locus_tag="ACHKFK_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO9051622.1"
                     /translation="MYQAHFSHNPLYCVDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGYLQGVPVACMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDERFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCELKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            28952..29716
                     /gene="yfeN"
                     /locus_tag="ACHKFK_001242"
     CDS             28952..29716
                     /gene="yfeN"
                     /locus_tag="ACHKFK_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO9051623.1"
                     /translation="MKKHLFTLTLSSVLAIPAVSHAEFKGGFADIGIHYLDWTSRTTE
                     KSSTKSHKDDFGYLELEGGANFSWGEMYGFFDWENFYNDRHDKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYSFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(29755..30681)
                     /gene="yfeR"
                     /locus_tag="ACHKFK_001243"
     CDS             complement(29755..30681)
                     /gene="yfeR"
                     /locus_tag="ACHKFK_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9051624.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAQALWDVVRDQGNALMAGREGDPLYQI"
     gene            30771..31769
                     /gene="yfeH"
                     /locus_tag="ACHKFK_001244"
     CDS             30771..31769
                     /gene="yfeH"
                     /locus_tag="ACHKFK_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO9051625.1"
                     /translation="MKLFRIPDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(31766..31984)
                     /gene="ypeB"
                     /locus_tag="ACHKFK_001245"
     CDS             complement(31766..31984)
                     /gene="ypeB"
                     /locus_tag="ACHKFK_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO9051626.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGHRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKNEGLTQLVQPLKRPL"
     gene            complement(31986..34001)
                     /gene="ligA"
                     /locus_tag="ACHKFK_001246"
     CDS             complement(31986..34001)
                     /gene="ligA"
                     /locus_tag="ACHKFK_001246"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO9051627.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KSNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLAQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPEPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDETEMLRLLGS"
     gene            complement(34072..35070)
                     /gene="zipA"
                     /locus_tag="ACHKFK_001247"
     CDS             complement(34072..35070)
                     /gene="zipA"
                     /locus_tag="ACHKFK_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO9051628.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPTQPVQQPVQQPAYQPQPEQPLQQPVSPQVASAPQPVHSA
                     PQPAQQAFQPAEPVAAPQPEPVAEPAPVTDKPKRKEAVIIMNVAAHHGSELNGELLLN
                     SIQQACFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQV
                     PSYGDELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANV"
     gene            35300..36061
                     /gene="cysZ"
                     /locus_tag="ACHKFK_001248"
     CDS             35300..36061
                     /gene="cysZ"
                     /locus_tag="ACHKFK_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO9051629.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVLQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPKLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36246..37217
                     /gene="cysK"
                     /locus_tag="ACHKFK_001249"
     CDS             36246..37217
                     /gene="cysK"
                     /locus_tag="ACHKFK_001249"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO9051630.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKLGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            37601..37858
                     /gene="ptsH"
                     /locus_tag="ACHKFK_001250"
     CDS             37601..37858
                     /gene="ptsH"
                     /locus_tag="ACHKFK_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO9051631.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            37903..39630
                     /gene="ptsI"
                     /locus_tag="ACHKFK_001251"
     CDS             37903..39630
                     /gene="ptsI"
                     /locus_tag="ACHKFK_001251"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO9051632.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            39671..40180
                     /gene="crr"
                     /locus_tag="ACHKFK_001252"
     CDS             39671..40180
                     /gene="crr"
                     /locus_tag="ACHKFK_001252"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO9051633.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(40223..41074)
                     /gene="pdxK"
                     /locus_tag="ACHKFK_001253"
                     /gene_synonym="thiJ"
     CDS             complement(40223..41074)
                     /gene="pdxK"
                     /locus_tag="ACHKFK_001253"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO9051634.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKDCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALTDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            41179..41547
                     /gene="yfeK"
                     /locus_tag="ACHKFK_001254"
     CDS             41179..41547
                     /gene="yfeK"
                     /locus_tag="ACHKFK_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO9051635.1"
                     /translation="MKKIICLVVTLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFVDKVASSSSITGKPYIVKMPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(41550..42461)
                     /gene="cysM"
                     /locus_tag="ACHKFK_001255"
     CDS             complement(41550..42461)
                     /gene="cysM"
                     /locus_tag="ACHKFK_001255"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO9051636.1"
                     /translation="MSTLEQTIGNTPLVKLQRMAPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(42596..43693)
                     /gene="cysA"
                     /locus_tag="ACHKFK_001256"
     CDS             complement(42596..43693)
                     /gene="cysA"
                     /locus_tag="ACHKFK_001256"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO9051637.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGD
                     RLYVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(43683..44558)
                     /gene="cysW"
                     /locus_tag="ACHKFK_001257"
     CDS             complement(43683..44558)
                     /gene="cysW"
                     /locus_tag="ACHKFK_001257"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO9051638.1"
                     /translation="MAEVTQLKRYYARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(44558..45391)
                     /gene="cysT"
                     /locus_tag="ACHKFK_001258"
     CDS             complement(44558..45391)
                     /gene="cysT"
                     /locus_tag="ACHKFK_001258"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO9051639.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFRKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(45391..46407)
                     /gene="cysP"
                     /locus_tag="ACHKFK_001259"
     CDS             complement(45391..46407)
                     /gene="cysP"
                     /locus_tag="ACHKFK_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO9051640.1"
                     /translation="MAVNLLKKNSLVLVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMGKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(46565..47356)
                     /gene="ucpA"
                     /locus_tag="ACHKFK_001260"
     CDS             complement(46565..47356)
                     /gene="ucpA"
                     /locus_tag="ACHKFK_001260"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO9051641.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            47636..48532
                     /gene="murQ"
                     /locus_tag="ACHKFK_001261"
     CDS             47636..48532
                     /gene="murQ"
                     /locus_tag="ACHKFK_001261"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO9051642.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHTQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSSTAEFAITPVVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     ADEAKKCLDQHGGFIRKALEKE"
     gene            48536..49960
                     /gene="murP"
                     /locus_tag="ACHKFK_001262"
     CDS             48536..49960
                     /gene="murP"
                     /locus_tag="ACHKFK_001262"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO9051643.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PDIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KARQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPTATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIECMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPLGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVSGFIFTTLFGCRNVNLD"
     gene            49963..50049
                     /locus_tag="ACHKFK_001263"
                     /pseudo
     CDS             49963..50049
                     /locus_tag="ACHKFK_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(50138..51037)
                     /gene="yfeX"
                     /locus_tag="ACHKFK_001264"
     CDS             complement(50138..51037)
                     /gene="yfeX"
                     /locus_tag="ACHKFK_001264"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO9051644.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRTLSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGYCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(51133..51708)
                     /gene="yfeY"
                     /locus_tag="ACHKFK_001265"
     CDS             complement(51133..51708)
                     /gene="yfeY"
                     /locus_tag="ACHKFK_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO9051645.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADSDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(51769..52218)
                     /gene="yfeZ"
                     /locus_tag="ACHKFK_001266"
     CDS             complement(51769..52218)
                     /gene="yfeZ"
                     /locus_tag="ACHKFK_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO9051646.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRIWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(52205..52630)
                     /gene="ypeA"
                     /locus_tag="ACHKFK_001267"
     CDS             complement(52205..52630)
                     /gene="ypeA"
                     /locus_tag="ACHKFK_001267"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO9051647.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            52844..53713
                     /gene="amiA"
                     /locus_tag="ACHKFK_001268"
     CDS             52844..53713
                     /gene="amiA"
                     /locus_tag="ACHKFK_001268"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO9051648.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKEEPLKTSNG
                     HSKPKAKKSGGRRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSRKR"
     gene            53717..54616
                     /gene="hemF"
                     /locus_tag="ACHKFK_001269"
     CDS             53717..54616
                     /gene="hemF"
                     /locus_tag="ACHKFK_001269"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO9051649.1"
                     /translation="MKPDAHLVKQFLLNLQDTICQQLSAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGINFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDEYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALNEFIKVRDWV"
     gene            complement(54622..55674)
                     /gene="eutR"
                     /locus_tag="ACHKFK_001270"
     CDS             complement(54622..55674)
                     /gene="eutR"
                     /locus_tag="ACHKFK_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO9051650.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEEAQPMMTAESISHQSYRRLLSRAREYMLENMSEPVTVLELCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(55720..56220)
                     /gene="eutK"
                     /locus_tag="ACHKFK_001271"
     CDS             complement(55720..56220)
                     /gene="eutK"
                     /locus_tag="ACHKFK_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO9051651.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(56233..>56529)
                     /locus_tag="ACHKFK_001272"
     CDS             complement(56233..>56529)
                     /locus_tag="ACHKFK_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HFO9051652.1"
                     /translation="TAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATYGIDAA
                     LKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQRA"
BASE COUNT        13937 a        15128 c        13921 g        13543 t
ORIGIN      
        1 agtgaaccat tttcaggata ttttctatga aagtaaactt aatgcttttt agcttatttt
       61 tatttgtctc tattatggca tgcaatgttt ttgcattttc tatttctggt ggtggtagtg
      121 aggcgagcta taaagagaca gaaaaaacgt cagtgatgac gacaacgcat tctacaaaac
      181 ttcagccatc acaggcgatt ttgtttaaga tgagagaaga tgcaccacca ttaaacctca
      241 cagaagacat cacgcctact tatccgacaa aggcgaatta tctcattcat cctttgcgat
      301 agcgtctgtc acctggcagg tgaacaatca tgtctgatgc tgatgtgaac acctgcccaa
      361 atgagccatc ctgacccact atcgcctgcc ataccccacc ataaagcgaa tcatcaagcc
      421 acaagccggg gcaagaaaca taaatatccc cgtcaccagt tcattaccgc cagttaacca
      481 catggtgaag taataaatga gtgcagcgag gatcaccatg ccgaagaaag aagagatcgc
      541 cgataccatg ttcagtacct gaccgatgta tccgcttacg cagacaatac acagggcgag
      601 aaacatccat aaataaatca tagctcaacc tcctgccgcg ctaaaaaacc tgtaacttca
      661 gtcactttaa tagtaagaca atccttaacc tctggcaatt tttgctaagc atcaatgaaa
      721 acagcatgtt aaatgcaaga ctgttgtgta cggaaaaata tttactttgc acgattaata
      781 atcagtagct gaaagcagtc agcgagtgat aaagtacaga taccgcattc atgctcctcg
      841 tggttatgtc ctgactagtc tttacactct ttacaggaac cattgtcgta catgatggcc
      901 taatcaattg agtgtaccgc tgtcgacatg gaatcttctg cgatacaaca aatcgtatct
      961 acagaaggta actatgtttc caaaatgcaa attttcccgc gagtttctgc atcctcgcta
     1021 ctggctcacg tggtttgggc ttggtgtact ctggctttgg gtacagcttc cttatcctgt
     1081 tctctgcttt ctcggcacgc gtatcggcac aatggcgcga ccattcctga aacgtcgtga
     1141 atctatcgcc cgtaaaaacc tggaactttg tttcccgcag cattctgcga aagagcgcga
     1201 gaagatgatt gccgaaaact ttcgttcact cggcatggcg ctggtagaaa ccggcatggc
     1261 gtggttctgg cccgacagtc gcgtacgtaa atggtttgat gtggaagggc tggataacct
     1321 taaacgcgca caaatgcaaa atcgcggcgt aatggttgtc ggcgtccatt ttatgtcgct
     1381 ggaactgggc ggtcgggtga tgggactttg ccaaccaatg atggccacct atcgcccgca
     1441 taataatcag ctgatggaat gggtgcagac ccgtggtcgc atgcgctcta acaaagcgat
     1501 gatcggcaga aataatctgc gcggtattgt cagtgcactg aagaaaggtg aagcggtatg
     1561 gtttgctccc gatcaggatt atggtcgtaa aggcagctcc ttcgcgccgt tctttgcggt
     1621 ggaaaatgtc gccacaacca atggcaccta tgtcctctct cgtctctctg gcgcagccat
     1681 gttgaccgta acgatggtaa gaaaagcgga ttacagcgga tatcgtttgt acatcacccc
     1741 agagatggaa ggctacccga cagatgaaaa tcaagccgct gcctatatga acaagattat
     1801 cgagaaagag atcatgcgcg caccggagca gtacctctgg atccaccgtc gctttaaaac
     1861 gcgcccggtg ggtgaatcgt cgttatacat ttaacaaacg gctctcactc tttccctcct
     1921 caaatcagcc ataataacct catggttttt atggcttttt tcttaaggtt ttttccttcg
     1981 ctgaatatct aattattacc gtcagaaatt aaactgtcgc cgatataaga cacctgtaag
     2041 ttactaaaag taaataagaa aatgcctctt gtctcgtttc agttttaggc gtacattagc
     2101 gccgtctcga gcagtttagc tcagaattct gggatgatgc ccacggtaaa atacaggacg
     2161 tttaaggtcg ttttgcccgg tgtcaactca ctgtatcagg tgtaataaag tcattcaggc
     2221 gtaacagtag ttacgcggag agatgtaaag tgaaatattt ctttatgggc atttctttta
     2281 tggtcatcgt ttgggccggt acttttgccc tgatgatcta aaagcaaact gtgcaatgaa
     2341 aaacaggagc catctggctc ctgtttgcat ttctgacgat ggtgtttatt ccgcgttttc
     2401 gtgaatatgt ttgctgctgg caggtagtaa accgtcggca cggaacatcg ctttaatccc
     2461 acgaatcgcc tgacgaatac ggtcgcggtt ttcaatcagt gcaaagcgca catgcgtgtc
     2521 gccgtagtcg ccaaagccaa tccctggaga gacacagacc ttcgcttcgt taagcaattt
     2581 cttggcaaat tccagcgagc ccatggcagc atacggttcc gggattttcg cccagacata
     2641 catcgaagcc ttcggcattt cgaccatcca gcccgcttca tgcagccctt tcaccagcac
     2701 atcgcggcgg cgtttgtact gttcggcaat atcgcgcacg cactgttgat cgccctccag
     2761 cgccgcaata gccgccacct gcaacggcgt aaaggtgccg taatcgtgat agcttttaat
     2821 acgtgccaac gcgctgacca gcgttttgtt accgaccata aagccgatac gccagcccgc
     2881 catgttgtag cttttcgaca gcgtaaagaa ctcgaccgcc acgtcgcgtg cacctggcac
     2941 ctgcatgatt gacggcgctt tccagccatc gtagacgatg tccgcatagg ccaggtcgtg
     3001 gaccaccagc acatcgtagc gtttcgccag cgccacaacc ttttcgaaga actccagctc
     3061 cacgcattgc gcggttgggt tcgacgggaa gccgaggatc atcatcttcg gtttcggata
     3121 actttcgcga atggcgcgtt ccagttcgtt gaagaaatcg acaccttcca ccagcggcac
     3181 tgagcgtacc tgcgccccgg caatcaccgc gccgtaaata tgaatggggt aacttggatt
     3241 cggcaccagc actgtgtcac cgtgatccag tgtcgccagc atcagatgcg ccaggccctc
     3301 tttcgaacca atagtgacga tggcttctga ttccgggtcg atttcaacgt cgtagcgatc
     3361 ctgataccag cgggaaatgg cgcgacgtaa ccgcggaatg ccgcgtgaag tggagtaacc
     3421 atgcgtgtcc gggcgctggg ccaccgtata taatttttcg acgatatgcg gcggagtcgc
     3481 accgtccggg ttacccatac tgaaatcgat aatatcttcg ccgcgccgac gcgcagccat
     3541 tttcagttca gcggtaatgt taaaaacgta gggcgggaga cgatcaatac gcgtaaagcg
     3601 acgttcaggg cgagtgtcag ccataatttc ctcagattaa cgttagcgcc cggaccgtcc
     3661 gagcgacgtc gccacgatgg tggctgtttt gaaaatagcc tgattaattt ctgtctgtcc
     3721 agcgttttag aacattttta tcaatggctt taaatgagga actccacttc aaaaacagga
     3781 aatttatcta cgctaacaag aaaattacca ctcattttat taacattata atttcaataa
     3841 atttaccaac cagatgtgca ttatcaagag atgcattgct aatacaccat ttatctttca
     3901 atcccctttt aaaattgtgg tttttcctca aaaacggcct gctggtcatc agccttcagg
     3961 ttacctatca tagaggttta atccttattc agagtcaccc gtgcacgaaa tattcaacat
     4021 gctgctggcg gtcttcgatc gggccgcgtt aatgcttatc tgcctgtttt ttctcatccg
     4081 tatccgcctg tttcgcgaac tgttgcacaa gtcggcacac tcaccgaaag agctgctcgc
     4141 cgttaccgcc attttctcgc tgttcgccct gttcagcacc tggtccggcg ttcccgtaga
     4201 aggctcgctg gtgaacgtac gtattatcgc ggtgatgtca ggcggaattc tgtttggccc
     4261 gtgggtgggc atcattaccg gcgtgattgc gggtattcac cgttatttaa ttgacatcgg
     4321 cggcgtaacg gcgatcccct gctttatcac cagcattctg gcgggttgta tatcgggctg
     4381 gatcaacctg aaaatcccca aagcacagcg ctggcgcgtc ggtattctcg gcggcatgtt
     4441 gtgtgaaacg ttgaccatga ttctggtgat tgtctgggca ccaactaccg cattggggat
     4501 cgatatcgtc tctaaaatcg ggattccaat gatcctcggc agcgtctgta tcggctttat
     4561 tgtgttgctg gtacaaagtg ttgagggaga aaaagaggcc agcgccgcgc ggcaggccaa
     4621 gctggcgctg gatatcgcca acaaaacgct gccgctgttt cgccatgtca atagcgagtc
     4681 attacgcaaa gtctgcgaaa ttatccgcga tgacattcac gccgatgcgg tggcgatgac
     4741 taataccgat catgtgctgg cctacgttgg cgtgggtgaa cataactatc agaatggcga
     4801 tgacttcatt agcccgacta cccgccaggc gatgaattat ggaaaaatca tcattaaaaa
     4861 caatgatgaa gcccaccgca caccagagat tcattccatg ctggtgatcc cattgtggga
     4921 gaaaggcgtt gtgaccggaa cgctgaaaat ttactactgc cacgcgcatc agataacctc
     4981 gtcattacag gaaatggcgg tcggtctgtc acaaatcatc tccacgcaac tggaggtgtc
     5041 acgcgccgag cagctgcgtg aaatggcaaa taaggcagag cttcgcgccc tgcaaagcaa
     5101 aattaatccc catttcctgt ttaacgcttt gaacgctatt tcatcgtcaa tccgcctgaa
     5161 tccggatact gctcgccagc tgatctttaa tctgtcgcgt tatctgcgct ataacattga
     5221 attaaaagat gatgagcaaa tcgatatcaa aaaagagctg tatcaaatta aagactatat
     5281 tgccattgag caggcccgct ttggcgacaa gctgacggtt atctatgata ttgatgaaga
     5341 ggtgaattgc tgcattccca gcctgctgat ccagccgttg gtggaaaacg ccattgtcca
     5401 cggtatccag ccttgcaaag gtaaaggcgt tgtcaccatc agcgttgcag agtgcggtaa
     5461 tcgggtacgt attgcggtgc gagataccgg gcacggcatc gatccaaagg tgattgagcg
     5521 ggtcgaagcg aacgaaatgc cgggcaataa aattggcctg ctgaatgtcc atcatcgcgt
     5581 gaagttattg tatggcgagg ggctgcatat ccgccgcctg gaaccaggaa cggaaattgc
     5641 gttttacatt cctaaccaac gcaccccagt cgcctcacag gctacgttat tgctttgagc
     5701 cggagtgata ttgtgaaagt catcattgtt gaagacgaat tcctggcaca acaggaactg
     5761 agctggctaa ttaaagagca cagccagatg gagattgtcg gcacctttga cgacggtctg
     5821 gacgtgttga agtttttgca gcataaccgc gtcgacgcca tttttctgga tatcaatatt
     5881 ccgtcgctgg atggtgtgtt gctggcacaa aacatcagcc agttcgccca taaaccgttt
     5941 attgtgttca tcaccgcgtg gaaagaacat gcggtagaag cgtttgaact ggaagcgttt
     6001 gactacattc tcaaaccgta tcaggagtca cgcattaccg ggatgctgca aaaactggaa
     6061 gcggcctggc aacaacagca gaccagcagt acgcctgccg cgacggtaac gcgtgagaat
     6121 gacaccatta atctggtgaa agatgagcga atcatcgtca cgcccattaa cgatatctat
     6181 tacgccgaag cgcatgagaa aatgaccttt gtctatacgc ggcgtgaatc ctacgtaatg
     6241 ccgatgaaca ttaccgagtt ttgcagcaag ctgccgccgt cgcatttttt ccgctgccat
     6301 cgctcatttt gtgtcaatct gaacaaaatc cgcgagatcg aaccgtggtt taataacacc
     6361 tatattctgc ggctgaaaga tctggatttt gaagtgccgg tcagccgcag caaagttaaa
     6421 gaattccgcc agttaatgca tctttaatcg ggaactttca tgaaagcacc aggcttgccc
     6481 gccgatcaac aatttttcgc cgatctgttc agcggcctgg tgcttaaccc gcaactactg
     6541 gggcgcgtct ggtttgccag ccagcctaac tcattgccag tgggcagttt atgtattgat
     6601 tttccccgtc tggatatcgt gctacgcggc gaatacggca atctgctgga agcaaagcag
     6661 caacgtatgg tggaaggaga aatgctgttt attccggcac gcgcggctaa tttaccggtc
     6721 aacaacaaac cggtgatgct gttaagcctg gtgttcgcac cgacctggct tggcttatca
     6781 ttttacgata gccgcaccac gtcgttgtta catcctgctc gccagatcca acttcccagc
     6841 ctgcaacgcg gcgaaggtga agcgatgctt accgccctca cccatcttag ccgttcgccg
     6901 ctggagcaga atatcattca gccactggtg ttaagtttgc tgcatctttg ccgtaacgtg
     6961 gtgaatatgc cgccgggcaa ttcgcagccg cgcggcgatt ttctctatca cagcatttgt
     7021 aactgggttc aggataatta tgcccagccg ctcacccgcg agagcgtggc tcagtttttt
     7081 aatatcacgc ccaatcatct gtcaaaactg tttgctcagc atggaacaat gcgttttatc
     7141 gagtatgttc gctgggtacg aatggcaaag gcaaggatga ttttgcagaa atatcatctg
     7201 tcgattcatg atgtggcaca gcgttgcggt tttccggaca gcgactattt ttgtcgcgtt
     7261 ttccggcgtc agtttggcct gacgccggga gagtacagcg cccgttttca gggctaacgt
     7321 cagaaggtta attccgtttc cagcagcgtc aggatgcttt gcgcatcttg cgcggcaaac
     7381 aacgactggc ggaagttttt attcaccagt ttgcgcgcca gctgcgagaa gactttcaca
     7441 tgattcatcc cttcgttagc gcccagcgtc agcatgatca ccagttcgac ttcgcccatt
     7501 tctgactgcc agtcaaccgg tttcaccaac cgggcaatgc tgatactgga atgacggatc
     7561 cactgtgatt tggtgtgcgg gatcgccacg ccaaaaccaa cgccagtagt aacaatctct
     7621 tcccgctgcc agacatcttc ttccagctcg aacggatgtt cagtacgccc gttaacaccg
     7681 aggttaccgc acaggaactg gatcgcctgc tctttattac taaaggactg atcaacaaag
     7741 atattctcta gtgccagcag tgggcgaacg tcttcttccg gcgcaaacgc ggtaagtaac
     7801 gcttcaattt cctgcgcgct gcggcactcg cacgcctgac gcgccagttc ccgacacgcc
     7861 tcgctatcca gttgacgaag ctggcttttc actgccggaa tacgcgggct actcatgctt
     7921 agctcatcca ggcccagccc tagcagtagc ggcagataac gactttcacc gcccagttca
     7981 ccgcaaatac ctacccattt gccgcgctga tgcgcagcgg taacaatctg ctgcaacatg
     8041 cgcaggaacg atggcgtaat cgggttatac agcggcgata cgcgcgggtt attacgatcg
     8101 accgcataca gatactgggt catatcgttg gagccgatac tgaagaaatc gacctcatcg
     8161 cagaagtggt cgatgatgta gcacaccgac ggcacttcca ccatgatacc cagcgtaatc
     8221 gtctctgcat gacgcaggcc atcgcgctta agctcaacga tcgctttttg gatctcaccc
     8281 ttcacccata agatctgatc gagactgtgg accattggga tcatcaactg ggcgttgccg
     8341 aaactggcag cgcgcaaaat cgcccgcaat tgagtacgga acaggccagc aaattccggg
     8401 taaatacgta ccgcgcgata gccgaggaac gggttctctt cctggggaat gttgaggtaa
     8461 ggaatgcttt tatcgccacc gatgtccatc gtgcggaaga taatcggctt gtcgcccgcc
     8521 gccagtagca cctgctggta cgcttcaaac tgttcctgct cgtccggcgc gctgtcgcga
     8581 tccatataga gcatttcggt acgaaacagc ccaacgcctt ccgcgccgtt ggcaaacgcg
     8641 cctggggctt ccagagctgt gccaatattc gccgcaatat caatacgctt tttatcacgg
     8701 gaataggcca gctgcgcggc agcttgcgcc tgttgttttt ggcgtttatc cgccagcgtc
     8761 tgcgccacct ggtaataacc gctcaccgca tcgttgggat taattgccag tacgccgcac
     8821 tgggcatcaa gcactgcagg ttgcccggca taacgggcaa tcgcatccag cggcaggccg
     8881 ctcagtaccg ggatcgccga agcgcgggcc agaatcaggg tatgcgaggt gcgcccggtt
     8941 ttctccagaa tcatgcccgc aagatttttt aaatcgaggc tcaaaaactg gcttggggtt
     9001 aaatcttcag ccaccagaat ggtcggtttt tcaagcacca gattattgcg cggttttagt
     9061 tccggccagg tgatatgcag caactgttcg ctgatatcgc gaatgtcgct gacccgctcg
     9121 cgcagataat cgctggcaga agcagaaagt ttggcacaaa tctgctccat attgctgatg
     9181 atcgccgccc ccagcccctg atgctgttct gccatcaggt gacggatatt gcctgcaaat
     9241 tcatcatcct gaatcagcga caaatgggcg ctgaggatag ttttgctttc gccgtcacgc
     9301 tcacgcaatt gctggttcag ttgctcggca agcgttgcca gactgtgctc cagtagggtg
     9361 gaatcttgcg cactcgcagg gatcacccga taactgtcga ggctgtcgct ctgtaacagg
     9421 gtcagtgtac ccacgccaac gccgcttgcc agcacgttgc cgtacagtaa atccgggttc
     9481 aggcggctta atgaacgcgg cagcggatgc gccgtcagtt ctgccagcgt cggctggacg
     9541 ctatcgctgt cgataaagcg cacctggata tactcttcca gcactcgccg cgcctgctct
     9601 tcatcgctac cgctaatgtt caggctgcaa ctgtcattaa ataaagtgcc agtgccaatc
     9661 agcgccagcg agcttttcgc atcggccttt gcgttctggc gatggttaat aaaagtgatt
     9721 tcgctttgcc actggctgca ctgctcttta agttcccacg ccggacgagc atgtagaccg
     9781 ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg ctccgggtta atcaggagat
     9841 catctgaaat ccgtcagttg aacaaccgtc tcacgggtaa gacgttgaat aagggcagat
     9901 aaaagttgct gcatctgcaa aatgtcgcgg cagtcggcaa tcgatgcggc gcaatgtccg
     9961 tggcgggttg ctggccccat caccactgtg ggtacgccag tgccggttaa gtgcaccgcc
    10021 ccaccgtccg tgccgccatt gctgaacata tctgactgca acggtacgcc aatttctgcc
    10081 gccacggttt cgatccaggc gataagtttt ggcggcgcaa tcagtgactt gtcgcttaac
    10141 accagcatcg gcccgttacc aatctggcga tggttagctg cgccataatc aaagtttttc
    10201 gcccagcagg cggtatcaag cacaatggcg acatccggcg acaccgcgcg ggtggcagtt
    10261 tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca ccagccacac ttccgcaggc
    10321 agttcggcgt cgtgcagctc acgcagtaac gtcaccagca gatagcaacc gaggcggtca
    10381 tcaaaggctt tccccatcac ccgctggtga gggagaacct gaaaagtggt atcgaaggta
    10441 acgcgatcgc ctggacgaat tcctgcctgc atcacttcgt cacaggagcg cgcgccaatg
    10501 tccacgcgca tggcgctgac gtcattcccc tgccgatcgc cgtcaagcag gcctggaatt
    10561 ttgcactctt cacgggtagt gatacgcacc gactgaagct ggcgggcagc catgcgtaca
    10621 ttgccaaccg gcaacacatc gatcgcccct tcgcgggaga tgctgcgcac cataaatccc
    10681 acttcgtcca tatgcgcaca gatcatcacc ttcggacctg tcgactcatt caggcggatc
    10741 agcaccgatc ccagaccatc aaatcgcact tctttttgca ggcgatccgc ttcttccagc
    10801 aggatctgcc gcacttcctg ttccgaggag gcgatcgcat ctgcctcgct caacgctttt
    10861 aatagcgata aatccattat gcctctcccg tgagcaacac tgttttcggc atggcgtaga
    10921 gcacctctgc gccttgcggg gtaaccagca caacatcttc gatgcgcacg cccccttgcc
    10981 ctggcaaata aatccctggc tccacggtca gtaacatacc tggctgtagc gtcgtggtgt
    11041 cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc gatagcatga ccggtgttat
    11101 ggccgaaata gtcgccataa ccggcttctg taatcacccg gcgcgcggca tcgtcaacct
    11161 gctggcagcg cacgccgggg cgaatcgcgg agattgctgc gagctgtgcc tgcaggacaa
    11221 tttgatagac gttaaacagc gggtgagatt cggcgctcac cccttcgcca ttcaccagca
    11281 aggtgcgcgt catatcagag cagtagcccc gatagagcgc accgaaatcg agagtgacaa
    11341 actcgcccgc tgtaacaatc ttgtcgctgg ctttgccgtg cggcagcgcc ccacgccagc
    11401 cgctggcgac aatggtgtca aaagaggctt tttctgcgcc ctgccggcgc ataaaccact
    11461 ccagttcagc ggctatctcg cattcgctca ttcccgcctg aataaagcgg cgaatatgct
    11521 ctgcgccacg atcggcaatc ccgcaggcaa ggcggatttt ctccacctct tctggcgttt
    11581 tgatttgccg cagcacatcc ggcgtggcgc tcaccagttt cgcattgagt tcggactgcc
    11641 agcggtgcgc ggtttcccaa ctcacctgct ggccttcaaa accgagcgtt tgcaactgct
    11701 catcggcaat gatttgtctg acgatggtgg caagcgtgtg catcgcgtcg agcaaatgca
    11761 gctggtaacc ttgcgtgcgg gcttctacat ccgcgtaata gcgcgaatcc accagaatgt
    11821 gcgcactttc acggctaatc accacataac ctgatccggt ggagatcccc agatgcggct
    11881 gtttgttctg ccgtgaggag agaagcaccg catccagttg ttgtgcctta agccagtcac
    11941 gcagcgaagc gagtaatgtc atctggattt ttccttctta caggctatcg attaacaatt
    12001 tgcctttacg gaacatcatc agacgcagga acaccaccat cagcgcagtt atgactgccc
    12061 ccagcgcgat ccccgccatg tagacgccga ggttagtgac cagcggccat gcccagatag
    12121 cggactccgg aaaccattgc actgcgccca gccagacagc ggcggttgag ccgacaatcg
    12181 cgccgaccat atacgacgga atggcggtga tagggctttc cagtgcaaac ggaatcgcac
    12241 cctcactgat ccccataaac gccaggaaca tcgcggtttt accctgcgga taaagctggg
    12301 cgttgaacaa gcgtttgcca gttaagcgac gatcgataat ggtcgccaga cccagaccaa
    12361 tcggcggaat aacgatagcg atagaacgtg cagtgaccgg taatacgtgg tcagtggtaa
    12421 agctgaaggc aacgaaacct gccgctttgt tgatcgggcc accaaggtcg attgctgtcg
    12481 ccgcggcgat ccccattgcg tacatcagcg cgcccttctc acctgcagcg gtcagcacgg
    12541 tacggatacc gccgttgatc cagccaccaa agggggtgat gacgtagtac atcgccagca
    12601 tgacaaaaat ggcggaaaga atcggtagca aaaatgtggt tttgaacgct aacaggaaat
    12661 ccggtaactg gattttctgg ttcatccact tcacgaggta accggcaacg atagaaataa
    12721 tcagcgcacc aatgaaggtg gacggcaccg gcgaagaggt cgcccattgc atcgtgctgg
    12781 gatcgaagtt cagcagttgc gtcggctggg tagacatcaa gccgccgata aatccagccg
    12841 ggaacgccag tttgccaccg atagagttcg ccacaaaagc agcaaacatc ggaatggcaa
    12901 aaccaaacaa cacaccgccg aaggactgcg acagccaggc aaatttcagt aaggagaggt
    12961 cgaaaccgga gaatttccca ctattaagag catccatgat gccgatatca gcaggaattt
    13021 taagccagct ataagcaatc agctgactga aagcgaggat cacaccgccc ataattaagg
    13081 tcggcaccat gcgtgaaatc cctgacatca catgctgcgg gagttcaccc cagaagctgg
    13141 atttagaggc ctgcggattc ttaactgccg ctgccgcacc ggatgcgcca tgcacaacgg
    13201 ttgcactgcg ttttttaatg gccataacaa ttatccctgt cgaatattac tgttgttcag
    13261 aggcaatcat ctcttcgatt tctttgatga ttcccgcagc gtttttaatt gcgtcctgca
    13321 aagtgatttc ataaacgtcg cgtgattcga aacgttcgtt atcttccggg gtaactgcca
    13381 cggagtggat gatgatggtc gcttcggcga tatcctgcgc cgtcaggcga ttctggatac
    13441 cgtccgcgcc ctgggtttca attttcactt cataaccggc ttctaccgcc gcttcttcca
    13501 gcgcctgagc ggccataaaa gtgtgagcca ggcccattgg gcaggcacat aaggcaatca
    13561 gtttcttact catcgttaat tcctcgttca gtgatagttg acgagtgaaa ttatgaacgt
    13621 gcaaaacaaa tcgccgttac cagacaaata atgcatttac tggagaatta atcccgctgt
    13681 acggaagtgt gacgcaggtc ggccttgtga gggggacaca aagaaaattt aattaactgt
    13741 atgatttaaa agattatcgg gagagttacc tcccgatata acaggaagga tttagagaat
    13801 gtgacctaag gtctggcgta aatgtgcacc ggaaccgaga agacccggat tgtcatgaac
    13861 gatgagatac accggaatat catggacata ttctttaaag cgccctttat cttcaaatgc
    13921 ggcgcggaaa ccagaggctt tgaagaactc aaggaagcgc ggcacgatac cacccgcaat
    13981 aaacacgccg ccaaatgtcc cgagattgag cgccagattg ccgccaaaac ggcccataat
    14041 gacgcaaaac agcgacaacg cgcggcggca atcggtgcag ctgtcagcca gcgcgcgttc
    14101 ggtaatatct tttggcttga gattttctgg caggcggttg tcggctttaa caattgcgcg
    14161 atacaaattc accagtccag ggccagaaag cacgcgttcc gccgaaacat gaccaatttc
    14221 cgcacgcaat atttcgagga taatgccctc ttcttcacta ttcggcgcaa aatcaacgtg
    14281 accgccttct cctggcaagc ttacccaacg cttatcgaca tggaccagat gcgcaacccc
    14341 aagccctgtt ccggcaccgt aaaccgcaat aggcttacct tctaccggtt ctgcgccacc
    14401 aaactgaatc agatgctctt ttttcagcat cgggatcgcc atcgatacag cagtaaaatc
    14461 gttaataatt tccagatggc taaaaccgag attctttttc atttcggcaa ttgagaacgc
    14521 ccaggtatgg ttggtcattg ccacccagtc accggtaatt gggcaagcga tggcaataca
    14581 gccgtctttc acctcgacct tatgttcttc aagataaacg cgaatgaccg cttcgaggct
    14641 ggggtaatca agccctgaat aggtcttagc ctgcgagatt tcaccactgg caatatcaca
    14701 cagagcaaga cgtgcgttgg tgccgcccac atcaccgact aatgcatact ttgtcattct
    14761 tcaactgctc cgctaaagtc aaaataattc tttctcacac tgtaaatacc tgggggcata
    14821 acaacaacgc cggaaaggca ggctccctgt aaatatcgat ctgggtcaca caattacttt
    14881 atcgtttcag caccaattgc agcgatgcct ttttgcaagc tgggcaaact aagtatctga
    14941 ccccgcataa ggaatagaac atgctccatc cgcgagccag aaccatgttg ttattatcgc
    15001 tccccgccgt ggcaattggg attgcgtcca gtcttattct gattatggtg atgaaaatcg
    15061 cctcggtatt acagaatttg ctctggcaac gactgccggg aactctgggg atagcccagg
    15121 attcacctct ctggatcatc ggtgtattaa cgctaacggg tattgcggtg gggttggtta
    15181 tccgtttcag ccagggtcat gccggaccag accccgcctg tgaaccgctg atcggcgcac
    15241 cggttccgcc ctctgcgcta ccaggactta tcgtagcatt aattctcggt cttgctggcg
    15301 gcgtcagcct ggggccggaa catccgatca tgaccgtcaa tatcgccctt gccgtcgcca
    15361 ttggcgctcg tctgttaccg cgcgtcaacc gaatggagtg gactatttta gcctctgccg
    15421 gaaccatcgg cgcgctgttt ggcacacctg ttgcggcggc attgatattt tcgcaaacct
    15481 taaatggcag taatgaagtt ccgctatggg atcgtctctt tgcgccgtta atggcggcag
    15541 cagctggtgc acttactacc ggattatttt tccatcctca tttttcactg cccattgcgc
    15601 attacggaca gatggaaatg accgatattc tcagcggtgc aattgtcgcg gcgattgcca
    15661 tcgcagcagg gatggtcgcc gtatggtgct taccacggtt gcacgagatg atgcatcaaa
    15721 tgaaaaatcc ggtgttcgtg ctgggtattg gcggatttat tctcggtatt ctgggggtta
    15781 ttggtggacc ggtttcgctg tttaaagggc tggatgagat gcagcagatg gtggcaaatc
    15841 aggctttcag caccagcgat tactttttgc tggcggtgat aaaacttgcc gccctggtcg
    15901 ttgctgccgc cagtggcttt cgcggtgggc gaatcttccc ggcagtgttt gtcggcgtgg
    15961 cattagggtt gatgcttcat gagcacgttc ccgccgttcc agcggcaata accgtttcct
    16021 gcgctatact cggcatcgtg ctggtggtaa cacgcgatgg ctggttaagt ctttttatgg
    16081 cggcagtcgt tgtacccaat accacattgc taccgctgct ctgtatcgtc atgctcccgg
    16141 catggctgtt attagcaggt aagccgatga tgatggtcaa tcgtccgaag caacagccac
    16201 cccacgataa cgtttagcaa aaaatgctct cctttatgat ttaagagtta tggcgatgat
    16261 acggcgcttt acctccccgt aatattgcgt taacaggccg ctgacggctt cgatcgtgaa
    16321 ggagaataac aatgttcagg tcactgtttc tggcggccgc cctgatggca tttaccccgc
    16381 ttgcggcaaa cgcaggtgaa atcaccctac tgccatcaat caaattacaa attggcgatc
    16441 gcgatcatta cggtaattac tgggacggtg gtcactggcg cgaccgtgac tactggcacc
    16501 gcaattatga atggcgtaaa aaccgctggt ggcgtcatga taatggctac catcgtggct
    16561 gggataagcg taaagcgtat gaacgtggct atcgtgaagg ctggcgcgat cgtgacgatc
    16621 atcgtggaaa aggccgcgga catggacacc gccattaaac gcatccgtca atggagcaca
    16681 atgcctgatg cgacgctagc gcgtcttatc aggcctacaa accagctctt taacgcgtag
    16741 ggcggataaa gcatttacgc cgcatccgcc aatggtgcca aatgcgacgc tcattcaact
    16801 acaatcccag tgccgttcct accagtaacc agatattcag cgccactacc agcaccacaa
    16861 tcacccagcc tgtctgtttt acgcgtttac tgttcaccag atcgcccatc aacttgccgt
    16921 cactggtgaa aatcagcaga ggaaccagcg ccagggcgat accaaaactt aacagcacct
    16981 gactcataac cagaatccgt gtcggatcta atcccatcag aatgacaata aatgacggca
    17041 gcatggtgac tgtacgacgc acccacagcg ggatatgaaa gcgaatgaag ccctgcatca
    17101 ctacctgccc cgccagcgtc cccaccaccg ttgaagacag tccggcagca accaggctta
    17161 atccaaagac cgttgccgca gcatggctta acagcggttg cagcgtcaga taagcctcat
    17221 caagatcggc aacaccagta tgaccagaaa agtggaacgc cgccgcagct gtagccatca
    17281 tcgccagatt gacaaaaccg gcaatcgtca tggcgatagc cacatcccat ttggtggcgg
    17341 aataacgttg ttgacgcgaa ccgccatgta aatgctgagt gagcgaagag tgcaaataaa
    17401 tcacatgcgg cataatcgtc gcccctaaca cgcccgctgc caggaagacc gcctccgaag
    17461 taggtaaact cgggattacc attcctctac ccagctgcgc caggttaggc tgggagaaaa
    17521 tcaactcgac aatgtaagca gcggcaacaa acaacagtaa cccgccaatc actttctcca
    17581 gcggtttttg cccgcgacgt tgcagcatta aaataaggaa agtcgcgatt cccgtcagca
    17641 ccgcgccctg caacaacgaa acaccaagaa tgagtttaaa accgatcgcc gcaccaataa
    17701 attccgccag gtcggttgcc atcgcaataa tttctgcctg aacccaatag aaccacacga
    17761 cgggacgcgg gtagtgatcg cgaatctgct ccgccagatt tttaccggtg gcaatcccca
    17821 gtttggccga gaggatttga atcagcatcg ccatcaggtt agcccaaacg acaacccaca
    17881 ataactgata gccgaagctg gcacccgcct gaatattggt cgcaaagtta ccgggatcga
    17941 tatagccaat cgccgcaatg aacgcaggtc ccattaatgc gagcctcatc ttgcgcgccg
    18001 cccgtccgct gctactctca acgcgatagt tcgtcatctt gtgcctctaa aacatagcct
    18061 ttgctatgtt tcatgctacg ttaaatgaga atgattatca aattcattta aatgggttgt
    18121 gatgatttct ctgatagacc aggattatga ctacgaaaag atagatgact cgaatgttat
    18181 ggttatgttt aatattagca catttacata accttccgct tccatacaca acatagcaga
    18241 aatgtatgac agatcactat ttttgaagct tgtcacagga cgtcattata gtgtgtgtca
    18301 gatctcgttt tccttaacca tgttacatag aatgtgcacg gaaatttaac ctgcctcata
    18361 tttggagcaa atatggaccg cgtccttcat tttgtactgg cacttgccgt tgttgcgatt
    18421 ctcgcactgc tggtaagcag cgaccgcaaa aaaattcgta tccgttatgt tattcaactg
    18481 cttgttatcg aagtgttact ggcgtggttc ttcctgaact ccgacgttgg tctgggcttc
    18541 gtgaaaggct tctccgaaat gttcgaaaaa ctgctcggtt ttgccaacga agggactaac
    18601 ttcgtctttg gtagcatgaa tgatcaaggc ctggcattct tcttcctgaa agtgctgtgc
    18661 ccaatcgtct ttatctctgc gctgatcggt attctccagc atattcgcgt attgccggtg
    18721 attatccgcg caattggttt cctgctctcc aaagtcaacg gcatgggcaa actggaatcc
    18781 tttaacgccg tcagctccct gattctgggt cagtctgaaa actttattgc ctataaagat
    18841 atcctcggca aaatctcccg caatcgtatg tacaccatgg cagcaacggc gatgtccacc
    18901 gtgtcgatgt ccatcgttgg tgcatatatg accatgctgg agccgaaata cgtcgttgcg
    18961 gcgctggtac tgaacatgtt cagcaccttt atcgtgctgt cgctgatcaa cccttaccgt
    19021 gttgatgcca gtgaagaaaa cattcagatg tccaacctgc acgaaggtca gagcttcttc
    19081 gaaatgctgg gtgaatacat tctggcaggt ttcaaagttg ccattatcgt tgccgcgatg
    19141 ctgatcggct ttatcgccct gatcgctgca ctgaacgctc tgtttgctac cgtgactggc
    19201 tggtttggct acagcatctc cttccagggc atcctgggtt acatcttcta tccgattgca
    19261 tgggtgatgg gtgttccttc cagtgaagca ctgcaagtgg gcagtatcat ggcgaccaaa
    19321 ctggtttcca acgagttcgt tgcgatgatg gatctgcaga aaattgcttc cacgctctct
    19381 ccgcgtgcgg aaggcatcat ctctgtgttc ctagtttcct tcgctaactt ctcttcaatc
    19441 gggattatcg cgggtgcggt taaaggcctg aatgaagagc aaggtaacgt ggtttctcgc
    19501 ttcggtctga aactggttta cggctctacc ctggtgagtg tgctgtctgc gtcaatcgca
    19561 gcactggtgc tgtaagacca tacataaaaa agccggggta attccccggc ttttttacgc
    19621 ctgttaatca gctaatggct gcgggcgacc aatcaaatac ccttgcagat attgcacccc
    19681 gagcttatgc aatagcgcct gctgctgttg cgtctcgaca aactccgcga ccacactcaa
    19741 tgacttcgct ttcgccagat cggtaattga tctcacaatc atcgcatcca gcgtgttcgt
    19801 gacgatatct ttcacaaaga cgccatcaat tttgatgata tcagcctgca aacgctttaa
    19861 ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc gcaatccgga aaccaaactt
    19921 atgcagctgc tcgatgttgt acatgctgct ttctgcgtta gaaaacgcct gctcctcggt
    19981 gatctcaaga atgaccgcct gcggggagat gtaataacgc ttaaacagac gaataatccg
    20041 cccggcaata ttcttttgca gcagcgtgag cggcattaaa ttgactgaaa cgcgtggacc
    20101 ttttttgtcg caagggtgtg ttgccagcca cttcaacaag gattccagca cttgcaaatc
    20161 aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga aacttatccg gggtcataat
    20221 gccgccgtca tacttcagtc gcgcgaggat ctcatcataa ccttcaccct ctttgttgcg
    20281 aattggctgg gcgtagagca gcaaatcacc ctgatctaac gccgtgcgaa tggtattcag
    20341 caatagcacc tgtttagtgg tttgcccgga aaccatctcc tctctgctat ccagcgccag
    20401 cacatgatga tgtgcgcagg attgctccgc cagccagctt aactgcccca acaagggttg
    20461 cagggtttcc tgatttccat caaaatgccc ccaggcagca ccatagccca tatccagccc
    20521 ggtattgttc cagtgaattt gccgactatt caggatgtta accatatgct ggagtcgccc
    20581 ttccgtttcc ggcccgctca gcactaacag cagttcacta cccggcaatt gatacaactt
    20641 ttcgttttcc tgcatcaacg gcagcagcgt acggtaaatt gagcggatac agtgaacgcg
    20701 cattattaag ccgtaatgac gactcataaa ctcaagatta tcaatgcgca ggcagcaaaa
    20761 actcttgccc gcctcttgct ccggcgcttg ctccaacgca cgaaagttgg gtagaagcgt
    20821 taacggatcg gtcagcgcct gcaaatgcca gcggcggtta agccattcac tacgatgata
    20881 aatacgcacc atatagagca ggcaaacact aaaggaaatc agtactgcga gaataaatgc
    20941 cagcgaatat tcggttagta ccccttgcaa aaagttctgg ttgtaattca gaaggcaaag
    21001 cgttgaaaca gcccaggtga gatttaaaaa cggatagcga agcttaccga caccgagggt
    21061 aaagatgata aagaaaacag gtaccaggta accggcaata aagtcatttt catacggtgt
    21121 gcacatcaga agtagcagca cgctaagagc tgccagccag cttaaggtaa atgcgcgttt
    21181 ctctttgctc aacgacggag cgatatccct acgccacaat atctgtgcaa agtggggact
    21241 tacaatcatg cgggtgagat agtaaaaaag catgttgtaa atcagcactg cggtgaaaag
    21301 gcttagcaaa tcaacgaccg tgaaaatggc atccgcatcg ccgaaaaagg tagatatctt
    21361 tagcggaaaa tcaaagaaat ttcccacaag atacatgctg catttgatgc caatcggcgc
    21421 caccaaacca agccagaaca gacgttgcca gacgtatcgg ctggtcaatc cataacgcca
    21481 acgcgtcccc aattgccagc gtaatattgc acaggcacac aggacagcaa acgcctggct
    21541 gccgatcatg acggcggtct gtaaaaaagt gagatgaagg ttccacagat tggtgcaaaa
    21601 catcccgcat aaaatgggaa cgacaccacg ccagccaaag ataaacaaga ttgacagcat
    21661 tacgcacagt ggcatccacg ccagaaagat ataattggaa tggataacag caagtggcga
    21721 aattaaacgg gatagctgaa taagtaccac ggtgagcgta aacgctagtg tgaatatctt
    21781 gatatttttt atcaggttat gctccacaaa catgagtaca ctactctcac tgatcggggt
    21841 tttcaggcac acaaataata agtttattcg catcgcgaag caaacagggg ccagcctgtt
    21901 gctgtttttt cagtcagtga tgcattcacc acacggcact gccaggatgt agactgtatt
    21961 acaaaagcgg caaaaggcag agacaaaaaa cccccgcttt gcagcgaggg tttgaaattt
    22021 ggtggagcta agcgggatcg aaccgctgac ctcttgcatg ccatgcaagc gctctcccag
    22081 ctgagctata gccccacgag tcgtttacgt accaaatttg ctgggtgcaa aatttggtgg
    22141 agctaagcgg gatcgaaccg ctgacctctt gcatgccatg caagcgctct cccagctgag
    22201 ctatagcccc atcacgtaaa gcttgtcgag ttgacgggcg gaatcatatg aattccgccc
    22261 gaatgtgtca acggcaaatt gccacgtgta gtttcaatcg ctgaaaaatc aggcaaatga
    22321 acaattttgg aaaaccgctc gcattcagta gttattcatg tcacggtttc ctgtaaagtg
    22381 gtgttataaa atgaactact aatagaccca catacattca gggaattgtt atgttcaagg
    22441 agaggatgac gccagatgaa cttgccagac tgaccggcta tagccgccag accattaata
    22501 aatgggtacg caaggaaggc tggacaacgt caccaaaacc aggcgtccag ggtggcaaag
    22561 ctcgactggt tcacgtcaat gaacaggttc gtgaatatat tcgcaatgct gaacgtccag
    22621 aaggtcaggg agaagcgcca gccctttctg gtgatgcgcc gcttgaagtg ttgctggtga
    22681 cactggcgaa agagatgacg ccagttgaac aaaaacagtt tacatccttg cttctgcggg
    22741 aagggattat cggattgtta caacgcttag ggattcgcga tagcaaataa tatgaaaaga
    22801 ttacgcaata aaatgaccac cgaagaactg gctgaatgcc tcggtgtggc taaacaaact
    22861 gttaaccgtt ggatcagaga aaaaggctgg aaaacggaaa aatttcccgg cgtgaaaggc
    22921 ggtcgtgcca gacttattct ggtcgaaacg caagtttgcg agtttattca gaacacgcca
    22981 gccttccata acacaccgat gcttctggaa gccgaagaac cccttgccga atacgcaccg
    23041 ggtattcgca ctcctgctta tcggcaaatc atcagcgcaa tagacaacat gactcacagt
    23101 gagcaagaaa aagttgcgca atttttgtca cgcgaaggaa ttcgtaattt tcttgctcgt
    23161 ctcgacatag atgaatcagc ataaataaaa aacggcaggg gtatctctcc tgccgtttat
    23221 cttttatgcg ctaattactg ctgattttcg cgttcagcaa taaaagccag cgctttgttg
    23281 atacgctcga tactgcgggt cttaccgatt gcgtgaacgg taacatccag cgccggagac
    23341 tgccccgcac cggttacggc gacacgcagc ggcataccaa ctttacccat acccacttcc
    23401 agctcatccg ccgtcgcctg aatagcgtga tgaacgtttt cagcggtcca gtcagtaatc
    23461 gcagtcagtt tgtcacgaac cacttccagc ggctggcgcg ctaccggacg cagatgtttt
    23521 ttcgcggcgt cggcatcgaa ctcagcaaaa tcttcgtaga aataacggca gctctgtgcc
    23581 atctctttca gcgtcttgca gcgttcgccc agcagtttca ccagatcagc cagctgcggg
    23641 ccgttacggg tatcgatatt ttcctgctca atgtgccact gtaagtgagt agcaacatac
    23701 tccggcggca acgcgttaat gtagtgatgg ttcagccaca gcagcttgtc ggtgttgaac
    23761 gcactggcag atttgctgac ggcattcaga gtgaagtatt taatcatctc ttcacgagtg
    23821 aagatttcct gatcgccgtg ggaccagccc agacgcacca gatagttcag cagtgcttct
    23881 ggcagataac catcatcacg atactgcatt acgctgaccg ccccgtgacg tttggacagt
    23941 tttttaccgt catcgccgtt gatcatagaa acgtgcgcgt aaaccggtac cggcgctttc
    24001 agagccttaa gaatgttaat ctggcgcggt gtgttgttga tatggtcttc gccacggata
    24061 acgtgggtga tttccatatc ccagtcatcg acaaccacac agaagttata ggttggggaa
    24121 ccatcggtac ggcggataat cagatcatcc agctcctggt tgctgaactc gatcggacca
    24181 cggatctgat cgtcaaaaac aacagaacct tcctgcgggt tagcaaaacg tacgacacac
    24241 ggctcatcat cagcgtgatg ctcatgactg tggcggcagc gaccgtcata acgcggcttc
    24301 tcacctttcg ccatttgctc ttcgcgcagc gcttccaagc gctctttaga gcaatagcat
    24361 ttataagctg tgccctcttc cagcatctga tcgatcaccg cgttgtagcg atcaaaacgt
    24421 ttggtctggt agtacggacc ttcatcccac tccaggctca gccagttcat gccatccata
    24481 atggcttcga tagcttccgg cgtggaacgc tcaagatcgg tgtcttcaat acgcagcaca
    24541 aactcaccgc cgtggttacg tgcaaaaagc caggagtaaa gagcagtacg cgcgccgcca
    24601 acgtgcagat agcctgttgg gcttggcgcg aagcgagttt tgattttcat gaaatggcct
    24661 tacgtttaga aagatgccga caaccggcaa atcctggaaa aattgagtgg gcgatattct
    24721 atcactcccg cctgattcct caatgtagtt cgggttgcaa ccttacgcat tattgttttc
    24781 gttgacaaat tgcgcaacac ggtctgtttt gcgatcatct tgcttaaatt tacgacgaac
    24841 gaacaatttc tttagaaaat gcgttgactc attttgaact ctccctataa tgcgactcca
    24901 cacagcgggg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    24961 gttcgatccc gtcatcaccc accaactact ttatgtagtc tccgccgtgt agcaagaaat
    25021 tgagaattgg gtgattagct cagctgggag agcacctccc ttacaaggag ggggtcggcg
    25081 gttcgatccc gtcatcaccc accactttct cgccagctag atttcttgta acaatgtgaa
    25141 gtaccgaagt gggtgattag ctcagctggg agagcacctc ccttacaagg agggggtcgg
    25201 cggttcgatc ccgtcatcac ccaccagttc gggtcgttag ctcagttggt agagcagttg
    25261 acttttaatc aattggtcgc aggttcgaat cctgcacgac ccaccaatgt aaaaaagcgc
    25321 cctaaaggcg cttttttgct atctgcaatt tgcgaaattg cctgatgcgc ttcgcttatc
    25381 agaccaacta tgtccggcag ttcccgtgcc tctcatctac tgcgccaacg ccgctagcag
    25441 tttaaccagc gcgggcgtca cctgctgcgt ttcataaaca atatataagt ctgccgggat
    25501 gcgttctttg agcggacgga atacgacacc gggccagttc atctgcgcgt agctgtccgc
    25561 tatcaatgtg ataccaatac ccatactgac catggcgagt accgtttgcg gttcattaac
    25621 ttcgcgaata acaaccggtg aaaatcccac ctgctggcaa actcgctgca aaaaatccca
    25681 gtcagtgtac acgggcggca ttgtaacgaa atactcgtca cgtagcgctt ccagcgggac
    25741 ggtggaaaat gatgagagat gatgctcttc aggcatcgcc accagaaacg ccgattcatg
    25801 caaccgtaag ctggtaaaac cagtcggtgg ttctgtcgcc attcgccaga tcccggcatc
    25861 aagttcgcgg cgttccagca aggccatttg catcgcaggc atcttttcgc gaaacagaac
    25921 ttcaacgtta ggattttctc taaggaatcg ccgcataacc gggcgcatcc gtccccacat
    25981 tgccgttccc acaaccccga gttcaatccg ccctgcttct ccccgaccta tttgttcaac
    26041 ccgagccaat acattatttg cattcaccag caatcgacgc gattcttcca tcaagatttt
    26101 gcccgcgtgt gtcagtacga cgctgcgcga atggcgaata aaaagctgcg tgccgagttg
    26161 attttccagc tctttaatat gaatactgag cggaggctga gacatattta aacgcgctgc
    26221 tgcgcgacca aaatgcaact cttccgctac ggcaagaaaa taacggagca acttaagatc
    26281 tgttctatat acgcgttcca taattaagtg ctccatataa aaaatatatc gtattaaatt
    26341 atcatggcat aaagtatttc accaccagat atccgacata cataaacacg atggataata
    26401 tacccggcaa tacatttgga acaggaatag gtaatcgcac gattgtaaac aaagcaccaa
    26461 tgccaaaacc aacgccagaa gaaaataaaa tatctttcac taattaacct ttatcataaa
    26521 agcagcccca aggaacagag ctgcgaatcc ttttaatgtg taactgaacg atgcgttatc
    26581 ttttcagggt catggttata tttaaaccca aagaaaaata tcactgcgag gaaaagagca
    26641 taccctgcaa acatcagcca aatggtatgc caatccttta cgccatccac cgagaagtaa
    26701 tccactgcca taccgctcag aatcgagcca acccatgcgc cgacgccatt taccatcgtc
    26761 ataaaaaggc cctgggcgct ggcacgaatg ctggaatcaa cttcctgttc gacaaagacc
    26821 gaaccagaaa tattgaagaa atcgaatgca caaccgtaaa caatcatcga cagcaacagc
    26881 aaaataaacc cggttggtga tggatcgcca taggcaaaga agccaaagcg cagcgtccac
    26941 gccaccatac tcatcagcat gacggtttta ataccgaaac gcttcaaaaa gaacggtatc
    27001 gtcagaatga aaccgacttc agccatctgt gaaactgaca gtaaaatgga cgggtatttc
    27061 accacgaagc tatcagcaaa ctcaggatta cgggcgaaat catgcaggaa tgggttgcca
    27121 aaaacgttgg taatttgcag caccgcgccc agcatcatgg cgaagaggaa aaagatagcc
    27181 atgcgtggat ttttaaacag cacgaaggca tccagaccca gcttgctggc aagcgatgta
    27241 gtcgcttttt tctccgcaac cggaatcttc ggcaaagtca acgcataagc cgacagcagc
    27301 aatgacgcac cggacgcgat atacagttgc agactactca attccagatg cagcaggctt
    27361 actgcccaca tggcgacaat gaaccccacc gtaccaaaaa cgcgaatggg cgggaaagcg
    27421 gtcaccgggt caagccctac ctgggcaaga caggaataag agacgctgtt cgataacgca
    27481 atagtcggca taaacgccat cgcattgact aacatcaccc aaaacatcat atccggatca
    27541 gttacggatg ccgcataaaa aagtacgccc gcacacacca ggtgacacag catgtatgca
    27601 cgttctgcac gcagccattt gtctgcgatg atccccatta taccaggcat aataatcgcg
    27661 gcgatccctt tggaactgta aaccatgcca acattagcgc cggtgaaatg aagggtatta
    27721 atcatgtaag aaccgagggt aaccagccag ctcccccaga taaaatattg caaaaaggac
    27781 attaccttta agcgcaaagc gatgctcata atcgtttcct tgtcgcatca tatggcctca
    27841 ctcagtaagg ccgtttattg ttgttttact caggcaattt tgcgcagaaa gccgcaaata
    27901 agattaataa agttttgctt tgagagttcc gctgctgcca gcgtttgggc atgagaaagc
    27961 ttcacgtcac tcagaccttc cgccatgttg gtaatcgcag agaccgcaac gactttaagt
    28021 tcgcaatggc gagctgaaat aacctcaggc accacagaca taccaacaac atccccacca
    28081 ataatttgca tcatgcgaat ttccgccgca gtctcgaaat tcggccccgg atacgagacg
    28141 aacacgccct ccgtcagagg gaacccctct tctttcgcca ctttttgtaa cagtgcgcgg
    28201 tattccgcat cgtaggcatt cgccagcgag aagaagcgct caccaaaacg ttcatcatta
    28261 agacccacca tcggcgttcc cggcatggtg ttgatgtgat ctttcaatgc gaccagactg
    28321 ccggccccca cttcaggacg cagtgagcca gccgcattgg tgcagaacag caactcgcag
    28381 cccagcaact taaaggtacg gattgcatcc gtcatgatgg tcatcccacg accttcgtag
    28441 aaatgtccgc gacctttcat gcacgccact ggcaccccct ggagataccc cagcaccagc
    28501 tcacccgcat gaccgtgtac ggtacttacc gggaacccag gcagcttttc gtaggaaatt
    28561 gcgaccgcgt tctcaatctg atcggccagc gcgcccagcc cggaacctaa aataaaggcc
    28621 actcgtggcg tgaaatcagg tttataagtc ttgataatat ctacgcaata cagtgggtta
    28681 tgagaaaaat gagcctgata catatatatc ctttttttgt agggtggaat ctaacgcctt
    28741 tttataacga gattgcccca cctcatacca atacactttt ttcatgtatc cgatagttat
    28801 tcgatatcga tatatttata ttttttcact attaataggc tgaaaaaact gtattggtac
    28861 tctcctttcc cttgcattaa caatatgccg cttttaatca acctttttat taagcaatca
    28921 actaataacg atcggcatag ggaattatgc tatgaaaaaa catcttttta ctctgacact
    28981 ctcttctgta ttggcgatac cagcagtatc ccatgcagaa tttaaaggcg gttttgctga
    29041 tataggtatt cattatcttg actggactag cagaaccact gagaaatcgt cgaccaaatc
    29101 acacaaagat gattttggat atctggaact ggaaggcggc gctaacttta gttggggaga
    29161 aatgtacggg ttcttcgact gggaaaactt ttataatgat cgtcatgata aaccgggtag
    29221 cgagcagcgt tatacattta aaaataccaa ccgtatttac ctgggtgaca ccggatttaa
    29281 tctctacttg catgcgtatg gcacctacgg ttctgcgaat cgtgtgaatt tccatgacga
    29341 tatgttcctt tacgggatcg gctacaattt caccggcagc ggttggtggt tcaaaccgtt
    29401 ctttgcaaaa cgctatacag accaaaccta ttataccggc gataacggtt atgtcgccgg
    29461 ttgggttgca ggttacagct ttatgctggg cagtgagaaa ttcaccctca ccaactggaa
    29521 cgagtacgag tttgatcgtg atgccaccta cgcggcaggt aatggcggca aagaaggtct
    29581 gaacggtgcc gtcgcacttt ggtggaatgc aacttcacac atcaccacag ggattcagta
    29641 tcgttatgcg gatgacaaac tgggcgaaga tttttaccag gatgcgataa tctactccat
    29701 caaatttaat ttctgattga ttactgccgg agctcccggc agtatgaatt cactctatat
    29761 ctgatacaac ggatcccctt cccgcccagc cattagcgca ttgccctggt ctcgtactac
    29821 gtcccataac gcttgtgcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa
    29881 ctggcgttca accactggcg taatgcgctt taccaccagt gggctacctt caggcaatgg
    29941 tagcgccagt gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt
    30001 cgccggatga ccaatctcct gcacaatatt cgcctgaata ccgttgcgcg ccagcgctgc
    30061 gtcaatcagc ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa
    30121 tgcctgccag ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa
    30181 gaaaggctcg gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac
    30241 gatgccaaaa tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc
    30301 gtgcaggaca aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat
    30361 aagatgcgcg gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc
    30421 catacgcccg gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa
    30481 agccaactgc tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg
    30541 aacaccagta tgattttcca gctccttcac actgtgactc actgccgact ggctcaggcc
    30601 gatacgctct cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa
    30661 ttgctttaaa gaataattca tctgtttttt tcatgaatgg atgcaataaa tcaattttat
    30721 ttctcaaact ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt
    30781 ttcgtatccc cgatcctttc accttaaccc tgatcacagt ggtgttgctg gcctctttct
    30841 ttccggccag aggcgatttc gtccccttct ttgaaaatct gaccaccgca gcgatagccc
    30901 tgctgttttt tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggcc
    30961 actggcgact gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggcg
    31021 tgctgtttgc ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct
    31081 acttgtgcat tctccccgcg accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg
    31141 gtaacgttgc ggcggcggtt tgttctgcgt cagcatccag cctgctgggg attttccttt
    31201 caccattgct ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg
    31261 tcggtaaaat tatgctgcaa ctgttgctgc catttgtgtt ggggcatctt tcccggccgt
    31321 ggattggtga ctgggtgtcg cgtaataaaa aatggattgc gaaaactgac cagacgtcca
    31381 ttctgttggt ggtttatacg gcattcagcg aagccgtcgt taacggtatc tggcacaaag
    31441 ttggctgggg atcattgctg tttatcgtgg tggtcagttg cgttcttctg gctatcgtga
    31501 ttgtagttaa cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta
    31561 tcgtcttttg tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt
    31621 tccccacatc ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat
    31681 tgatggtctg tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc
    31741 agcaggaaag cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg
    31801 tcagcccctc gtttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgcag
    31861 ggaactcatc tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgat
    31921 gccctttgta tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt
    31981 ccatctcagc tacccagcaa acgcagcatt tccgtttcgt cgatgacttc aatgcccagt
    32041 tcctgcgcct tcgccagttt agatcctgca gcttcacccg ctatcaccag atcggttttc
    32101 ttcgacacgc tgcccgcgac tttcgccccc agttcgacca gtcgagcttt agcgtcatca
    32161 cgcgacatct ggcttaagct gcccgtaagc accacggttt taccagcaaa cggactgtca
    32221 atctcttccg cattgataac gatcggctca ggccagtgaa caccttccgc caacagctcg
    32281 ctgatgacat tgcggttgct ttcttcggca aagaagttgt gaacgtggga ggcaacgaca
    32341 attccaacat caggcacctt ttgcagctct tcaatcgacg cggcttccag cgcttccagc
    32401 gtgccgaaat atgccgccag acctgctgcg gtagcctcgc cgacttcacg gatgccaagt
    32461 gcatagagga agcgggcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt
    32521 tgtgccgatt tcggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac
    32581 agatccgccg gagtgtggac atattctttt tcaaccagtt gatcgatgat tttgtcgccc
    32641 attccgtcaa catccatcgc acggcgggaa acaaagtgtt tcagagactc tttacgctgc
    32701 gcaccgcaaa tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcgacg
    32761 tcagaaccac ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt
    32821 tcagaaagca cgacgttaac cacctgcggg atcacatcgc ctgcgcggcg aatcaccact
    32881 ttatcgccaa tgcgtaaacc aagacgttcg atttcgtcag cattatgcag tgtagcgtta
    32941 ctcaccaaca cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca
    33001 gtacgcccaa cctgaaactc gacgtcacgc acaaaggtca tctgttcctg cgccgggaat
    33061 ttaaacgcta ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgtgcc
    33121 agtgagttga ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg acggtcttct
    33181 tctactttgt ggtagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg
    33241 ctgaccggca aaccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc
    33301 tcgccacctt ccagaacacc gacgccgtag cagaagaaag tgagcggtcg cttcgctgta
    33361 atacgcggat caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaacact
    33421 ttcccgcccg tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac
    33481 acttcaccac gcacttccag acgcgccggg atattctcac cgtgtagctt cagcggaatg
    33541 gcgcgaatag tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgagtc
    33601 gcggcactga ctaaaacgcc attttcatac agaatactga cggcaagacc atccagcttc
    33661 agctcacagc accaggtgac tttctcgttg cttttcagac ggtcctgcac tcgtttgttg
    33721 aaggcaagaa agctttcttc atcaaaaacg ttatccagcg acagcattgg cacttcatgg
    33781 cggatctggc tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc
    33841 gtaatcagtt ctggatgttt ggtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat
    33901 tcagcgtcgg gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc
    33961 gtcgttcgca gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa
    34021 aaacccccga caagcggggg ttcgaagagg agttaatttg ccttaagtgt atcagacgtt
    34081 ggcgtctttg acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat
    34141 acggcgctga tcgtcaagca ctacaccgcc cacttcatcg gcaatatgct gcgcagattg
    34201 cagcatcagc ttaaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt
    34261 gacacccgga gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt
    34321 cgccaggctg aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatgtt
    34381 catatcgcca aaaatgaagc acgcttgttg aatgctgtta agaagcagtt caccgtttaa
    34441 ctcgctaccg tgatgcgccg cgacgttcat gataatcacc gcctctttgc gcttcggttt
    34501 atccgtaact ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc
    34561 aggctggaaa gcctgttgtg ccggttgcgg tgctgaatgc actggctgcg gcgctgacgc
    34621 gacctgtggc gaaacaggct gctgcaacgg ctgttcaggc tgcggctgat aggcaggctg
    34681 ctgcacaggt tgctgcaccg gctgcgttgg acgcggagca tgttgcggcg gtacctgcgc
    34741 ttcaggcggc tgctggaccg gttgacgcgg ctgcgcagac gcataaggcg gttggtactg
    34801 gtgttgcggc gacggacgag cagcctcatg ctcctgggcg ttagccgggg catgattcac
    34861 gcggtgaaca cgaacctcac caacgccctc atcatcttcg acatcctcgt cataagaatc
    34921 gtcgtcacgt tttgacttca ttcgttttaa tggccgatcg cggaacatag aagatcgttc
    34981 tttacggctg gtccagaaac catgtaccag taaagcgatt atggcgatcg cgccaacaat
    35041 gattaatatc agacgcaaat cctgcatcat tatattctct gttgttctaa caccttgcca
    35101 ccacggcaaa catttactca ctaagagtat ttgccgatta cctcaagtgc aagtgcacta
    35161 ttaactttca cagcacaaag atagatgaaa tcgtgctttt tgctgttttt tcgaacatat
    35221 cctaactgtc cattgcgcaa ttacccggta aaatacgcag aattttcctg ggattggtca
    35281 aaaggagctc atcctgatta tggtttcatc attcacatct gccccacgca gcggttttta
    35341 ctattttgcg caaggctgga agctcgtctt gcaacctggg attcggcgtt tcgttatttt
    35401 accgctgctg gtcaatattt tgttgatggg gggcgcattc tggtggctct ttacgcaact
    35461 cgatgtctgg atcccgaagc tcatgagtta tgttccggac tggttacaat ggctgagcta
    35521 tctgttgtgg cccctggctg tcatctctgt gctgttagtg tttggctatt tcttctcaac
    35581 gattgctaac tggattgctg ctccgttcaa cggtttattg gctgaacaac tggaagcaag
    35641 actgactggc gctacaccgc cagataccgg aattttcggt atcatgaaag atgtgccgcg
    35701 aatcatgaaa cgcgaatggc aaaaatttgc ctggtatctg ccgcgcgcaa ttgtattgct
    35761 aattctttac ttcatccctg gcattgggca aaccgtcgcg ccggtactgt ggttcctgtt
    35821 tagcgcctgg atgttagcca tccagtattg tgattacccc ttcgataacc acaaagtgcc
    35881 gtttaaagag atgcgcaccg ccctgcgcac acgcaaaatc accaatatgc agtttggtgc
    35941 tttaaccagc ctgtttacga tgatcccgct gcttaatctg ttcattatgc ccgttgccgt
    36001 ttgtggcgcg acggcgatgt gggtcgattg ctatcgcgat aaacacgcca tgtggcggta
    36061 acaatctacc ggttattttg taaaccgttt gtgtgaaaca ggggtggctt atgccgcccc
    36121 ttattccatc ttgcatgtca ttatttccct tctgcatata gatatgctaa atccttactt
    36181 ccgcatattc tctgagcggg tatgctacct gttgtatccc aatttcatac agttaaggac
    36241 aggccatgag taagattttt gaagataact cgctgactat cggtcacacg ccgctggttc
    36301 gcctgaatcg catcggtaac ggacgcattc tggcgaaggt ggaatctcgt aaccccagct
    36361 tcagcgttaa gtgccgtatc ggtgccaaca tgatttggga tgccgaaaag cgcggcgtgc
    36421 tgaaactagg cgttgaactg gttgaaccaa ccagcggtaa taccgggatt gcactggcct
    36481 atgtagctgc cgctcgcggt tacaaactta ccctgaccat gccagaaacc atgagtattg
    36541 aacgccgcaa gctgctgaaa gcgttaggtg caaacctggt gctgaccgaa ggtgctaaag
    36601 gcatgaaagg cgcaatccaa aaagcagaag aaattgtcgc cagcaatcca gagaaatacc
    36661 tgctgctgca acaattcagc aatccggcaa accctgaaat tcatgaaaaa accaccggtc
    36721 cggagatctg ggaagatacc gacggtcagg ttgatgtatt tattgctggc gttgggactg
    36781 gcggtacgct gactggcgtc agccgctaca ttaaaggcac caaaggcaag accgatctta
    36841 tctctgtcgc cgttgagcca accgattctc cagttatcgc ccaggcgctg gcaggtgaag
    36901 agattaaacc tggtccgcat aaaattcagg gtattggcgc tggttttatc ccggctaacc
    36961 ttgatctcaa gctggtcgac aaagtcattg gcatcaccaa tgaagaagcc atttctaccg
    37021 cacgtcgtct gatggaagaa gaaggtattc ttgctggtat ctcttctgga gcagctgttg
    37081 ctgcggcgtt gaaactacaa gaagatgaaa gctttaccaa taagaatatt gtggttattc
    37141 tgccgtcatc gggtgagcgt tatttaagca ctgcattgtt tgccgatctt ttcactgaga
    37201 aagaactgca acagtaatgc cagcttgtaa aaaatgcgta aaaaagcacc tttttaggtg
    37261 cttttttgtg gcctgcttca aactttcgcc cctcctggca ttgattcagc ctgtcggaac
    37321 tggtatttaa ccagactaat tattttgatg cgcgaaatta atcgttacag gaaaagccaa
    37381 agctgaatcg attttatgat ttggttcaat tcttccttta gcggcataat gtttaatgac
    37441 gtacgaaacg tcagcggtca acacccgcca gcaatggact gtattacgct cttcgtgcgt
    37501 cgcgtctgtt aaaaactggc gctaacaata caggctaaag tcgaaccgcc aggctagact
    37561 ttagttccac aacactaaac ctataagttg gggaaataca atgttccagc aagaagttac
    37621 cattaccgct ccgaacggtc tgcacacccg ccctgctgcc cagtttgtaa aagaagctaa
    37681 gggcttcact tctgaaatta ctgtgacttc caacggcaaa agcgccagcg cgaaaagcct
    37741 gtttaaactg cagactctgg gcctgactca aggtaccgtt gtgactatct ccgcagaagg
    37801 cgaagacgag cagaaagcgg ttgaacatct ggttaaactg atggcggaac tcgagtaatt
    37861 tcccgggttc ttttaaaaat cagtcacaag taaggtaggg ttatgatttc aggcatttta
    37921 gcatccccgg gtatcgcttt cggtaaagct ctgcttctga aagaagacga aattgtcatt
    37981 gaccggaaaa aaatttctgc cgaccaggtt gatcaggaag ttgaacgttt tctgagcggt
    38041 cgtgccaagg catcagccca gctggaaacg atcaaaacga aagctggtga aacgttcggt
    38101 gaagaaaaag aagccatctt tgaagggcat attatgctgc tcgaagatga ggagctggag
    38161 caggaaatca tagccctgat taaagataag cacatgacag ctgacgcagc tgctcatgaa
    38221 gttatcgaag gtcaggcttc tgccctggaa gagctggatg atgaatacct gaaagaacgt
    38281 gcggctgacg tacgtgatat cggtaagcgc ctgctgcgca acatcctggg cctgaagatt
    38341 atcgacctga gcgccattca ggatgaagtc attctggttg ccgctgacct gacgccgtcc
    38401 gaaaccgcac agctgaacct gaagaaggtg ctgggtttca tcaccgacgc gggtggccgt
    38461 acttcccaca cctctatcat ggcgcgttct ctggaactgc ctgctatcgt gggtaccggt
    38521 agcgtcacct ctcaggtgaa aaatgacgac tatctgattc tggatgccgt aaataatcag
    38581 gtttacgtca atccaaccaa cgaagttatt gataaaatgc gcgctgttca ggagcaagtg
    38641 gcttctgaaa aagcagagct tgctaaactg aaggatctac cggctattac gctggacggt
    38701 caccaggtag aagtgtgcgc taacattggt acggttcgtg acgttgaagg tgcagagcgt
    38761 aacggcgctg aaggcgttgg tctgtatcgt actgagttcc tgttcatgga ccgcgacgcg
    38821 ctgcccactg aagaagaaca gtttgctgct tacaaagcag tggctgaagc gtgtggctct
    38881 caggcggtta tcgttcgtac catggacatc ggcggcgaca aagagctgcc atacatgaac
    38941 ttcccgaaag aagagaaccc gttcctcggc tggcgcgcta tccgtatcgc gatggatcgt
    39001 aaagagatcc tgcgcgatca gctccgcgct atcctgcgtg cctctgcttt cggtaaattg
    39061 cgcatcatgt tcccgatgat catctctgtt gaagaagtgc gtgcactgcg caaagagatc
    39121 gaaatctaca aacaggaact gcgcgacgaa ggtaaagcgt ttgacgagtc aattgaaatc
    39181 ggcgtaatgg tggaaacacc ggctgccgca acaattgcac gtcatttagc caaagaagtt
    39241 gatttcttta gtatcggcac caatgattta acgcagtaca ctctggcagt tgaccgtggt
    39301 aatgatatga tttcacacct ttaccagcca atgtcaccgt ccgtgctgaa cttgatcaag
    39361 caagttattg atgcttctca tgctgaaggc aaatggactg gcatgtgtgg tgagcttgct
    39421 ggcgatgaac gtgctacact tctgttgctg gggatgggtc tggacgaatt ctctatgagc
    39481 gccatttcta tcccgcgcat taagaagatt atccgtaaca cgaacttcga agatgcgaag
    39541 gtgttagcag agcaggctct tgctcaaccg acaacggacg agttaatgac gctggttaac
    39601 aagttcattg aagaaaaaac aatctgctaa tccacgagat gcggcccaat ttactgctta
    39661 ggagaagatc atgggtttgt tcgataaact gaaatctctg gtttccgacg acaagaagga
    39721 taccggaact attgagatca ttgctccgct ctctggcgag atcgtcaata tcgaagacgt
    39781 gccggatgtc gtttttgcgg aaaaaatcgt tggtgatggc attgctatca aaccaacggg
    39841 taacaaaatg gtcgcgcctg tagacggcac cattggtaaa atctttgaaa ccaaccacgc
    39901 gttctctatc gaatctgata gcggcgttga gctgttcgtc cacttcggta tcgacaccgt
    39961 tgaactgaaa ggcgaaggct tcaagcgtat tgctgaagaa ggtcagcgcg tgaaagttgg
    40021 tgatactgtc attgaatttg atctgccgct gctggaagag aaagccaagt caaccctgac
    40081 tccggttgtt atctccaaca tggacgaaat caaagaactg atcaaactgt ccggtagcgt
    40141 aaccgtgggt gaaaccccgg ttatccgcat caagaagtaa ttcttgccgc agtgaaaaat
    40201 ggcgcccatc ggcgccattt ttttatgctt cagccagagg cggcaaaatc aattcatcgc
    40261 tctcatgctg ctgggtatag cgcatcactt ccagtacgcg caaccccgcg cggtgcaccg
    40321 catcggttaa cgccttccct ttcagcaatc cactgataag ctgagcacaa aacaggtcgc
    40381 cagtcccttt caggtcagtt ttaacccggg aatgggaaat gacattcacg ctgtcggcac
    40441 tgaccaccac cacctgcatc tcctgatttt cttcattgcc ggaggcgctg gtaatcacca
    40501 cccactttaa tgtgtctgaa agcagacttt ttgcggcagc aatggcactg tcgagatcgc
    40561 ggcaatcttt accggtcagg atttccagct caaagatatt gggggtaatt ccctgtgcca
    40621 gcggcagtaa atattgtcga tacgcctcgg gaaggtcagg tttgacataa attccgctat
    40681 caatatcgcc aatcaccgga tcgaccatga tcaataggtc aggatggtct ttgcgtagcg
    40741 cagtcagcca ctcggcaagg attttgattt gcgatgccgt tcccatatag cccgtggtta
    40801 cagcacgaag ttggcgcagc gcatcacgct cctgaagcgc acgcaaataa ccgctaaacc
    40861 attcatccgg aatcgcacca ccgtagaaag tgtcataatg cggcgtattg ctcagcaata
    40921 ccgtcggcac ggcaaagaca ttcaggccgt tctgctttat agcaggcacc gcaatgctgt
    40981 tgcctacgct gccgtacact acctgcgact gcacggcgac gatatccgcc tgcagtgccc
    41041 tgctcttatc gttaaacaac aacaaactac tcatttaatt ttttctcctt gccgatgatc
    41101 ctcatcgtat tccagccgaa actttacctg attctggcag tcaaatcggc tattacaaaa
    41161 caaggataag gtaattcagt gaagaaaatc atttgtctgg tcgttacgct gctaatgaca
    41221 ctccccgctt atgcgaagtt aacggcccat gaagaagccc gtatcaacgc catgctggag
    41281 ggattagcac agaaaaagga tttgatattt gtgcgcaacg gtgatgaaca tacctgcgat
    41341 gaagcggttt ctcatctgcg cctgaagctg ggcaataccc gtaatcgcat tgatacagcc
    41401 gagcagtttg ttgacaaggt ggcctcgtcg tcatcgatta ccgggaagcc gtatatcgtg
    41461 aagatgcccg gtaagagcga tgagaacgca cagccttttt tacatgcgtt aattgcgcag
    41521 acggataaaa cggtgcctgc gcaataatct taaatccccg ccccctggct aaaatgctct
    41581 tctccaaaca ccccggtaga aaggtagcga tcgccgcgat cgcagatgat cgccaccacc
    41641 accgcgccgg ggttagcttt tgccacccgc agagctccgg caaccgcgcc gccggagcta
    41701 acgccacaga atattccttc ccgcactgcc agttcgcgca tggtgttttc cgcatcgcgc
    41761 tgatgaatat ccagcacctc atccaccaga gaagcgttga aaatccccgg cagatattcc
    41821 gcaggccagc ggcgaatgcc gggaatactg ctgccttctt ccggctgcag gccaacaatg
    41881 gtcaccggtt tggattgttc gcgcataaag cgcgacacgc cggtaatggt gccggttgtt
    41941 cccatgctgg agacaaaatg ggtaatacgc ccgccagttt gctgccagat ttccggcccg
    42001 gtagtggtgt aatgggcgta agggttatcg ggattattga actgatcgag caactttcct
    42061 tcgccgcgat tcgccatctc cagcgccaga tcgcgtgcgc cttccatgcc ctgttctttg
    42121 gtgaccagaa tcagttctgc accgtaggca cgcatcgccg cacggcgttc ctggctcatg
    42181 ttgtcgggca tcagcaattt catgcgatag cctttaagcg ccgcaatcat tgccagcgca
    42241 atgccggtgt taccactggt ggcttcgatc agcacatcac ccggtttaat ctcgccgcgc
    42301 ttttccgcct cgacaatcat cgaaagcgct gcacggtctt ttaccgatcc cgccgggtta
    42361 ttgccttcca gttttaacca cacttcactg ccgttatccg gcgccattcg ctgcaacttc
    42421 accagaggcg tattgcctat tgtttgttct aatgtactca cgatctctgt ccatacgtgg
    42481 tgttgcctga tgtgacactt gcgcgtctta tcaggcctac aggttacaaa accttgccat
    42541 taaaaagccc ggatcgcggg aagcgcctcc gggcgtttaa cattcactca acctatcagg
    42601 cgctttgtgc gagagcaagt tcctcatcgc gggtttcaat acgctcatcg ccgttataca
    42661 gccgcgcatg ttgcagacca acgtataaac gatcgccacg ctgcggggca tcgtcgccat
    42721 gcatcacgac cgtcagcggt tcgttgtacc accccagcgg ctgcaccact aattgggtgt
    42781 agtgaccttt cgggctggct tccagtacct gtacgggtag cggcgaatcg aggctggtac
    42841 ggcggctgat atccacttcc cacgggcgca ggaagagatc caccggcccc tgatacgcgg
    42901 gggtgtatcc cagcggccag cgatgtgcgc caacgtggaa ctgcccgccg cgaatggttc
    42961 cctgcaggcg gtttacctcg cccataaact ccagcacaaa acgagtcgcc ggttcacgcc
    43021 atacctgatc cggtgcgtca gcttgttcaa tattgccctg actcatcacc actacacgat
    43081 cggctacttc ggtcgcctct tcctggtcgt gggtcacgaa cacgctggtg aatttcagtt
    43141 cttcatgcag ttgacgcagc cagcgacgca gctctttacg cacctgcgca tccagcgcgc
    43201 caaacggttc atcaagcagc aaaatttgcg gttccacagc cagcgcgcgt gcaagcgcca
    43261 cacgctgttt ctgaccgccg gaaagctgcg ccggataacg atccgccaga tgagcaagct
    43321 gcaccatctc cagcaatttt gtcactttcg ctttgatcgc cgccgcattc gggcgttcac
    43381 gccgtggcaa caccgtcagg ccaaaagcga tattgtcaaa caccgtcata tggcgaaaca
    43441 gcgcgtaatg ctggaacacg aaaccgactt tacggtcccg cgcgtgcaga cggctcacgt
    43501 cggtaccgtg aaagcgaata tgcccactgg tttgatgctc cagcccggcg ataatgcgca
    43561 gcagcgtagt tttcccggaa ccggacggcc caagcaacgc gaccatctga ccagaaggaa
    43621 tatccagtga gatatcgttc agcacctggg tgcgaccaaa agacttctta atattggcaa
    43681 tctcaatgct catgatgttc ctcctgctgc gcgcgttttt cctgattttc caggcgccac
    43741 tgcaacatac tctttaaaaa cagggtgata atcgccatca gcgtcaacag cgcggcggcg
    43801 gtaaaggagc cgacggtgtt gtagtcctgc tccagcaatt caatctgtaa cggcagcgac
    43861 agagtttcgc cacgaatcga gccggaaacc accgataccg cgccaaactc gccaatcgcg
    43921 cgggcgttgg tcagcaccac gccgtaaagc agcgcccagc ggatgttcgg cagcgtgacg
    43981 cgacggaaca tctgccagcc ggacgcgcca agtaaaatcg ccgcttcgtc ttcctggctg
    44041 ccctgactta acattaccgg caccagctcg cgcaccacaa acggacacgt cacgaagatg
    44101 gtgaccagca ccatacccgg ccaggagaac ataatttgca gattatgttc atccagccag
    44161 ccgcccagcg ggccgttaga gccgtagaac agcagataca ccagaccagc aaccaccggc
    44221 gacacggcaa acggaatgtc cagcagcgtc agcaatagct ggcgtccggg gaagttaaag
    44281 cgcgtcacca gccaggccag cagaataccg aacaccaggt ttaccggtac ggcaatcaac
    44341 gcgatcatca ccgtcagcca gatggcgtgc agcatgtccg gatcggccag attttgtaac
    44401 actggcatca gccctttgct gaacgcctgt acgaagatat aaatcatcgg taccagcagg
    44461 atgaacgccg ataccagcat cccgatgcca atcagaaacc atttgcccca gttaatcggg
    44521 cgcgcgtaat aacgcttcaa ttgtgtcact tccgccatta atgacctacc acacgccgac
    44581 caaagcgact ttgcagagtg ttaattgaga acagcaacag cagcgatgcc gcgaggatca
    44641 ccgaggcaat cgcgctcgcc gccgggtaat caaactcctg taagcgaaca aagatcatca
    44701 gcgacgtcac ttctgttttc caggcgatat tcccggcgat aaaaatcacc gcgccaaact
    44761 caccgaggct gcgggtaaac gacagcgcca cacccgccac cagcgccgga gatagttccg
    44821 gcagcaccac tttgcgaaaa ctctgccagc gtgttgcgcc gagcgtttct gccgcctctt
    44881 catattccgg gcctaactct tccagcaccg gctgcacggt gcgcaccaca aacggaatgc
    44941 tggtaaaggc catcgccacc gcaatcccca gccaggtata ggtgactttg atatcaaact
    45001 tcgccagcca ttcaccgtaa aaaccgttca cggaaaagag cgaggccagc gttaaaccgg
    45061 cgactgccgt tggcagcgca aagggtaaat ccatcagcgc atcaagcagc gtgcgacccg
    45121 gaaagcgata gcgggttaag atccacgcca tcagcagacc gaaaacgccg ttaaaaatcg
    45181 atgccacaaa cgccgacagc agcgtcactt tataggctgc gaccacctgc gggttggtga
    45241 tcacctccca gtactgcgcc cagctcatct gggccagttg catcaccagc gcggagagcg
    45301 gcagcagcaa aatcaggcac acaaacagca gactggtgcc gaggcttaag gtaaagcccg
    45361 gcagcacgcg tcttgaagaa actgcaaaca ttacttacgc cccgccgcta acagcttgtc
    45421 taactcgccg ccgctggtga agtgggtttt catcacttcc ggccaggagc caaatttatc
    45481 ttccacgcgg aacagctcgg tctgcgggaa tttatctttc agtttgccca tgacttccgg
    45541 gttatttacg cggtagtaat agtcggtgat gatggtttgc gcctgcgggc tgtagagcca
    45601 gttcaggtag gcttttgccg ctttttccgt gccgttggcc tgtacgtttt tatccaccca
    45661 cgccaccggg aattccgcca gaatgttggt tttcggaatc accacttcaa agccctgcgc
    45721 ttcatactgt ttacggatgt tgttcacttc cgactcgaag ctgatcagca catcgcccag
    45781 gccgcgctcg gcaaaagtgg tggtcgcgcc acgaccgcca gtatcgaaca cttcaacgtt
    45841 tttcaggaac tgggtcataa actgttcggt tttggcttta tcaccaccgt cggctttgtc
    45901 cgctgcgccc catgccgcaa ggtaggtata acgcgcgtta cccgacgttt tcgggttcgg
    45961 gaaaatcagc ttcacgtcag agcgtaccag gtcgttccag tcgtggatat tcttcgggtt
    46021 gcccttacgc accaggaagc ccatggtgga gtagaacggc gagctattat tcggcaggcg
    46081 cgactgccag tccgctggga tcagcttgcc tttatcgtgc aggatttgta cgtcggtcac
    46141 ctggttataa gtgacaacgt cggcttttaa accctgcaaa attgccagcg cctgttttga
    46201 tgatccggca tgagattgtt taatcgtcag tttgtcgcca ccgttatctt ttgcccattg
    46261 ttgctcaaac ggcgggttca gggcggcaaa cagctcgcgg gagacgtcat aagaactgtt
    46321 cagcagttcc gttgcctgta catggcccgc cagtagcaga gaagcaacca gcacgagtga
    46381 gttctttttc agtaagttaa cggccattgc gcacccttat aaatttaatg actttctaat
    46441 agccatcata tttataacgg acgtgaaagg agtaacggtt ttatataccg tttggtgatt
    46501 tggaagttga aaagggaata agacgaaatc ctccccacaa atgcagggag gaaacagagg
    46561 tgaatcagat gccgacgcta accgtctccg gcagtgtgct gccgccatca atcacattct
    46621 gtgtaccggt taaatagctg gattcatccg atgcgaggaa ggccgccagt tcgccgactt
    46681 ccagcggatc ggcgaggcga cgcatcggga ttgctttcgc catttcagtc agcaccgatt
    46741 ctggatcttc cgggttcgac tggcgggcaa tgctttccgc cattggcgtg cgaacgtatc
    46801 cagggcagat ggcgttaacg cgaataccag actgcgcgta ctccaccgcc agcgatttag
    46861 tcaggccaac aatcgccgct ttcgttaagg cgtacgccgt ttcgcccggg tctgccacca
    46921 tatcgccagt gactgaagac atcatcacaa tccggccatc tttgcgggca atcatctccg
    46981 gcagcaccgc cttcgtgacg ttccatacgc cttttatatt gatatcaata tggaaatcgc
    47041 gatcttcatc gctcatatca aggaaactgc ccagacgaca aacgcctgcg ttattcacca
    47101 gaatatcaat gcgcccttct ttttccttag cgcgcttaat agctgcggct accgacgccg
    47161 ggtcacgcac atcggcgaca accgccgtac agcgatgacc acggccacac agttcgtccg
    47221 ccagcttttc gatctcaggg gagatatcca gcaagattag gtttgcgcca tgacgcgcaa
    47281 aagttctggc aattccttcg ccaattccct gcaatgcgcc cgtaatcagt gctgtcttgc
    47341 ccgtgagttt acccatttta atgctctcct tgttaataca gagcgttaca ctccattccc
    47401 tctgaaaata caggataaac aaaatattgc tgcggatcag ctcactaatt caggaaaatc
    47461 tcaatttagt cgctgcttta tgcagcacct caagcgttac gtcagcgcgc aatcattttc
    47521 agtgacatca catgagtaat attcggactt aatgattcct ttgtgtctat caaatcggga
    47581 tatatgatcc catttatctg caattatttg atcgcaaata ttaaggtcac caccgatgca
    47641 acttgaaaag atgattaccg aaggctcgaa cgccgcctcg gctgaaattg accgcgtatc
    47701 aacgctggaa atgtgccgga ttatcaacga tgaagataaa accgtaccac tcgccgttga
    47761 gcgcgtactg ccggatatcg ccgcggcgat cgatgttatc cacacccagg taagcggcgg
    47821 cggccgtctg atttatctcg gtgcgggaac atccggtcgt ctggggattc tggatgccag
    47881 cgaatgtccg ccgacctacg gcgtgaaacc gggcctggta gttggtttga ttgctggcgg
    47941 cgaatatgcc attcagcacg cggtggaagg cgcagaagat agccgggaag gtggtgttaa
    48001 tgatctgaaa aatattaatt taaccgcgca ggatgtggtg gttggtattg cggccagcgg
    48061 acgcacgcct tatgtgattg ccgggctgga atacgcgcgc caactcggct gccgtacagt
    48121 gggcatttcc tgtaatccgg ggagtgccgt ttcatccacc gcagagtttg ctattacgcc
    48181 ggtagttggt gccgaagttg ttaccggttc ttcgcgaatg aaagcaggca cggcgcagaa
    48241 actggtgctc aacatgctct ccaccgggct gatgattaaa tccggcaaag tgttcggcaa
    48301 cctgatggtc gatgtggtcg ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt
    48361 caaaaacgct accggatgta atgccgaaca agcggaagcg gcgttaattg cttgcgagcg
    48421 caactgtaaa acggccattg tgatggtact gaaaaatctc gatgccgatg aagctaaaaa
    48481 atgcctggat caacacggcg gttttattcg caaggctctg gaaaaggaat aacccatggc
    48541 taaagagatc agcagtgaac ttctgaacac cattcttacc cgggtcggcg gaccgggaaa
    48601 tatcgccagt tgtggtaact gtatgacgcg cctgcgtctg ggtgtacatg acagttcatt
    48661 ggttgatccc gatatcaaaa cgctggaagg cgtgaagggc gtcattttga ccagcgatca
    48721 ggtgcaggtg gtttttgggc caggcaaagc gcatcgtgcc gcaaaagcca tgagcgagct
    48781 gctgggcgag gcaccggtac aggatgccgc agaaatcgcc gcccagaata aacgccagtt
    48841 aaaagccaga caaacttccg gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac
    48901 gccgctgatc cccggtttta ttgccgccgg tctgctgctg gggatagcga cgttaattgc
    48961 cacggtgatg cacgttccgg cagatgctca gggcacgcta cccgatgcgc tgaattttat
    49021 gaaggtgttc agcaaaggcc tgttcacctt cctggtcatt ctggtgggct ataacgcggc
    49081 ccaggcattt ggtggcacgg gcgtaaatgg cgcaattatc gctgcgctct ttttgcttgg
    49141 ttataaccca actgcaacca cgggttacta cgccggattt cacgatttct ttggtctgcc
    49201 catcgatccg cgcggcaata ttatcggcgt gttgattgcc gcatgggcct gcgcccgcat
    49261 cgaatgcatg gtgcgccgct ttatgccgga cgacctcgac atgctgttga cctcattaat
    49321 caccctgctg atcaccgcta cgcttgcgta cctgatcatt atgccgctgg gcggctggct
    49381 gtttgaaggc atgtcgtggc tgtttatgca cctgaacagt aatccgctcg gttgtgcggt
    49441 tttagccggg ctgttcctga tcgccgtggt atttggcgtg catcagggct ttattcctgt
    49501 ttatctggcg ttaatggaca gccagggatt caacagctta tttcccatcc tttcaatggc
    49561 aggcgcaggc caggtgggcg cggcgctggc gctctactgg cgggcgcaac cgcacagtgc
    49621 attacgcagt caggtccgcg gggcgattat tcccggtctg cttggcgttg gcgaaccgct
    49681 gatttacggt gtcaccctgc cccgcatgaa accgtttgtt accgcctgtt taggcggcgc
    49741 ggcgggcggt ttgtttatcg ggctgatagc ctggtggggt ctgccgatgg gcttaaacag
    49801 cgcctttggg ccgtctggtc tggtggcgct gccgctgatg acttccgcgc aaggcatcct
    49861 tccggcaatg gcggtttacg ctggcggtat tctggtggca tgggttagtg ggtttatttt
    49921 caccacactc tttggctgcc gtaacgtcaa tctggactga ttatgaaacg gacaatgctc
    49981 tatctttctc tgctggctat tagctgtagc gtcagcgccg caaaataccc tgttcaggca
    50041 gaaagtagtt gatcaagtgt atgcggcgtt aaagcagtaa taaaaaggcc agtcagttgt
    50101 gttactggct ggcctttaat caatgaatca gaaacgctta cagcgccatc aacttgtcca
    50161 gcgacggcgc gaagtaatag ccgccagtga ccggtttggt gaaacgcaac atcgcatcac
    50221 gcttaccatc ggtatcgcca aacatgctca gcagttgctg ctcaatgtta tgcagacgcg
    50281 cgcagtaggc gcagaagtac agaccgtgag tgccactggc ggtgccgtac ggcaggctct
    50341 gacgaacaat cttcagccct ttgccatctt ctttcagatc aacgcgggtc aagtgagagg
    50401 tttccggacg ttcgtcgccg tcaatctctt cgttggcctc tttggtgcgc ccaatcatca
    50461 tctcttgatc gtgaacgctc atccgattga gctgcttcag gttgtgctcc caacgctgca
    50521 caaacacata gctgccgccc gcatccacgc cgtctttgat aacagccact tcgcggcgcg
    50581 tctcttcacc cgccgggttt tctgtaccgt caacaaagcc gctcaggtca cgctcttcaa
    50641 cccaacggaa gccatggatc tcttctttca cttcaatgca gtaaccaaag gcttccatcg
    50701 ccgcctgagc gacagagaag ttcacgtcgt gacgcagaga aagaatgtgg atcaacacat
    50761 cgaactgggt cgtcggtgca agacctttac cgtagcccgg aaaatctttc agctcttcag
    50821 ccccaacgcc gccgctcaga gtgcgccagg tattgttacc aaaggcaacc accgcaccaa
    50881 gatgcgcgtc cgggaatttc gcttcaaaag ttgccagttt gtcggcaaat gttttactgg
    50941 ccgcacgcag ggcgtcaact tcccctttca cgttggcttc gatccaaatc gccgcgcggc
    51001 aatgttctgg caaaatgcca ctctgaacct gagacattgt tcctcctgaa aataataatg
    51061 ccacgcaatc cgtggcattg ttgggcgcta ttttacgtgt tttttcgcgg cggcggtttg
    51121 ttcagacgca aattaacgcc gccagataat tttactgact ttccagtttt tcagggtatc
    51181 gtccgaaggc attaaccctt caggaccact ccattccccg ctgaactggt agctaatatg
    51241 ttgactgcct tcagctttac attcgacggc acgattatca tcactgtcgg ccttttggca
    51301 attgccaaat gctttgctgt aaaggtcact aaacggtgta ccgattttaa caccggtgtc
    51361 agcaggaata tcgctatcca gcacatcaat gcggctgatc gtgccttgat caccgttaat
    51421 caccatcgcc acgttgtcgc ctttcatcac ttcaaaaaag cgcaccacgt tgccgttcgc
    51481 ggttttcatt ccgctgcgca ggcgataatc gccatcaagc gcatcagcaa tggcttgttc
    51541 ttgcagtggt gtggacgccg ttaattcacc cacgccctgc tcgctcactt tggtggatga
    51601 tccaaaccag ttccacgggt tagcggccga ccagttaact gacgacatcg tggaacagcc
    51661 ggtcagcatc aacggcatag cgcataacat taaacgcagc gatttcatgt cacttccttt
    51721 ggttattcaa taacgttgct tggagtgcaa attcaccaaa aagtgccgtt attctttttc
    51781 ttcattaaaa caagcgcgta aacgtcgatt ggtcagcagc cagattaacg ccacgatatc
    51841 cgccactacc agcgccagac cgataccgct aacggattca ccgttcagcc acagccacgg
    51901 ttgccaacaa agtaaaacca cttgcgccaa cagcaacaga aaatagagca catgccagat
    51961 acggggaaat gaagcccgcc gaccactcag caaaaatgcc agcaccgccg gaataccggg
    52021 aatcaacccc agccagaaat tatcgtgatc gggataaaac agatttagca gcgcagtacc
    52081 ctgctcgcgc gacgctccgg caatgacaaa cagcacccag gttcgcgcct gaagcaatag
    52141 cacaagccag aagagcaagg gtaaacgcag acgaccgtgc gcatcataat ggacaggatg
    52201 aaactcagta ctcttcatct tcaatcaaac gcttacccag actcagcacg tcggcgtgtt
    52261 catatcccag gcgttcatac attccgagca ccatgtcgtt atcttccggc acgttgatct
    52321 gaattttcgg acagccgcga gcaatcagct ttttctccaa ccgattaagc aacgcattag
    52381 caatcccacg cccacgaaac tcgggatgca cgccaagata ataagcagac ccgcgatgcc
    52441 cgtcataacc gcccatcacc gttccgacca cttcaccgtt cacctcagcg accagaaaca
    52501 aactgacgtc atggttcatc ttacgctcaa tgtccatttc cggatcgttc cacggacgca
    52561 gcaagtcgca acgctcccaa agggtgatga cctcttcgaa atcttcctgg cgaaatacgc
    52621 gtatctccat ggtattcgtt acctttttgc gggttaaaag gctgattatg gcgtgaacgg
    52681 tcgaattagc caatatctga cgaaaatcgg ttgaaaaagt ggcataatgg ggagttgtca
    52741 actattgaaa tgaaaagtaa aacaattctc aacagcaaac cgtcgtaacg gattacgcga
    52801 tacgatataa catctggaac tttattatta caactcaggc cgtatgagca cttttaaacc
    52861 actaaaaaca ctcacttcgc gccgccaggt gctgaaagcc ggtttggctg ccctgacgtt
    52921 gtcaggaatg tcgcaagcca tcgccaaaga agaaccgtta aaaaccagta acggacacag
    52981 caagccgaaa gcaaaaaaaa gcggcggcag acgtgtcgtt gttctcgatc caggccacgg
    53041 cgggattgat accggagcga tcggacgcaa cggttcgaaa gaaaaacatg tggtgctggc
    53101 aattgccaag aatgtccgtt ccatattgcg taatcatggc attgatgcgc gtttaacgcg
    53161 ttctggcgat acgtttatcc cactgtacga tcgcgttgaa atcgcccata aacatggcgc
    53221 agatctgttt atgtcaattc atgccgacgg ctttaccaac ccgaaagctg ccggtgcttc
    53281 ggtatttgcc ctctctaacc gcggtgcaag tagcgcaatg gcgaaatacc tgtctgaacg
    53341 cgaaaaccgc gccgatgaag ttgccggtaa aaaagcgact gacaaggatc acctattaca
    53401 gcaagtgctg tttgatctgg tgcaaaccga tactattaaa aacagcctga cgcttggctc
    53461 gcatattctg aagaagatta agccggtgca taaactgcac agccgcaata ccgaacaagc
    53521 cgcgtttgtg gtgttgaaat cgccgtcggt tccgtcggtg ctggtggaaa cctcgtttat
    53581 caccaaccca gaagaagagc ggctgttagg cactgcggcg tttcgccaga aaatcgccac
    53641 agcgattgcc gaaggcgtga tcagttattt ccactggttt gacaaccaga aagcacactc
    53701 gagaaagcgg taagtgatga aacccgacgc acacctggtt aaacagtttc tgctcaacct
    53761 tcaggatacg atttgtcagc agttgagcgc cgtcgatggc gcagaatttg tcgaagatag
    53821 ctggcagcgc gaagctggcg gcggcgggcg tagtcgggtt ttgcgtaatg gtggtgtttt
    53881 cgaacaggca ggcatcaact tttcacatgt ccacggtgag gcgatgcctg cttccgccac
    53941 cgcccatcgt ccggaacttg ccgggcgcag tttcgaggcg atgggcgttt cactggtagt
    54001 gcatccgcat aatccgtatg ttcccaccag ccacgcgaat gtgcgctttt ttattgccga
    54061 aaaaccgggt gcagagcccg tctggtggtt tggcggcggc ttcgatttaa cccctttcta
    54121 tggttttgaa gaagacgcca ttcactggca ccgcaccgcc cgtgacctgt gcctgccatt
    54181 tggcgaagac gtttatccgc gttacaaaaa atggtgcgac gaatacttct acctcaaaca
    54241 tcgtaacgaa cagcgcggca ttggcgggct gttctttgat gatctgaaca cgccagattt
    54301 cgaccactgt tttgccttta tgcaggcggt aggcaaaggc tacaccgacg cttatttgcc
    54361 aattgtagag cgacgaaaag cgatggccta cggcgagcgc gagcgcaatt ttcagctcta
    54421 ccgtcgcggt cgttatgtcg agttcaatct ggtctgggat cgcggcactc tgtttggcct
    54481 gcaaactggc gggcgcaccg agtcgatcct gatgtcaatg ccgccactgg tacgctggga
    54541 gtatgattat cagccaaaag atggcagtcc agaagcggcg ttaaatgagt ttattaaggt
    54601 cagggattgg gtgtaactcc ctcaccccca ctcccgcatc cgctgatgca gcgtcagtga
    54661 cggcttctcg gcaaacagtt gctgataatc cgtggcaaat tgcccaagat gccagaatcc
    54721 ccactgcatg gcggcgtctt ttaccgttgt gctttgtgac cacggactta tgagttcgcg
    54781 gcgcacggcg ttcaggcgaa tacgtttcag ccacgcgttc ggaccaatgc ccaaaatggc
    54841 gtgaaatgcg ttttgtaatg tgcggcggct gacgtgcaat tgattgcaca actccaacac
    54901 cgttaccggt tcagacatat tttccagcat atattcacgg gcgcgggaaa gcaatcgacg
    54961 gtaactctga tgactgatgc tttccgccgt catcatcggc tgcgcttctt ccagcatcgc
    55021 ccccatcgcc attagcaaat tatcccccag cacttttcgc actgcaggct gatggagatt
    55081 ttccgggttc tcgcaaaacg tcgccagcgc ctgttggaca aagccccaca gcgcggcttt
    55141 atgctgctct ttcacttcca gcgccgactg gctacgcagc atatgtaata cccgatccgg
    55201 attatgcaaa aagttagcct gacgagtgat gacatcttca gaaagcacta cgccaaggat
    55261 cgtgtaatcg tccggcgtac ttaattcaaa ctcggttcca ccagggcgcg tcgcaatttc
    55321 tgcgcttccc agacattgcg aaccgataaa tccctgctca ccgcgcgtcg ccggaatgcc
    55381 aaaccagaac gagttcggcc agaccaggca cgactgacgc agcgccagac cagtgtattc
    55441 acgaaaaacc tgaatatcat cgagtagaat ttccgtaaat tcaccatgaa acttgcccgg
    55501 atgcagctga tcgtaaatct gctgccaggc ggtaattgtc aaagcatgtt catagacgtc
    55561 cgttgtctgt cgttgatgaa cattatccac ttcgaccttc ggcgtgagct tcaggttttc
    55621 gggtaagggt tcatgataaa gatggtgcaa attggctgta cgggtctttt tcatgatgtt
    55681 aatgccgggt gttgtaggac acccggcacc tccgacaggt taatggggct tgagacggta
    55741 acgactactg cgtttacgta acgtcccggc agaaaagagc tgttcgagcg catttctggc
    55801 tttttccagc ggccagccaa agtgggcagc cacttctcct gccgtcattc cctgacgtac
    55861 tgatgttaac agcgccaaca actcgtcagc agattccgcg acgataactg gcgcgtcggg
    55921 ttcctttacg ggttgcttcg gctggcggtt aaagccagta accagccact gggtgtcatc
    55981 gtctggccga ccgatctcct tacggctgat gacacgcccg gtacgcatcg cggcagcaca
    56041 acctgcgtcc agcgccgcac gacacgccgc cagatcgcct tccaccacca gcgttaggcg
    56101 accagggtca agcacttcgt gactgagcag acgaacgtta gctgctttga gcatggcatc
    56161 agctgcatct atcgcggcga ccatgccgtc cacttccagc aatcccagtg cattgatcat
    56221 cggcaacctc cgttacgcac gctggattgg attacgcgcg atttccagca ctgcatcggt
    56281 aaaggcgtta caggctgctt tacacgcggc ctggctaccg gttaaaaatg ctgccgagta
    56341 gttggtttca gacggtggcg ggacataggt caccagctgc acgtcggcag atttcaacgc
    56401 tgcatcaatg ccgtaggtcg cttccagcgg cggtgccacc agatacgcca tcggatcgcc
    56461 aagcgtgatc ccggcggttg atgagagata agaaccggta cgcgaaacta catgtgccag
    56521 gaaggctgt
//
LOCUS       DAZGJT010000019        54608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZGJT010000019 DAZGJT010000000
VERSION     DAZGJT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.019"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..154
                     /locus_tag="ACHKFK_001273"
     CDS             <1..154
                     /locus_tag="ACHKFK_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020237521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pas-pac-pac sensing his kinase"
                     /protein_id="HFO9051653.1"
                     /translation="ITTTSTPERQFSISVLDDDNKENIPTDTAPCFTIGIPDEYTKAI
                     ELLNIK"
     gene            332..1255
                     /gene="yicL"
                     /locus_tag="ACHKFK_001274"
     CDS             332..1255
                     /gene="yicL"
                     /locus_tag="ACHKFK_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO9051654.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLIGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(1259..2077)
                     /gene="nlpA"
                     /locus_tag="ACHKFK_001275"
     CDS             complement(1259..2077)
                     /gene="nlpA"
                     /locus_tag="ACHKFK_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO9051655.1"
                     /translation="MKLTTHHLRAGAALLLAGVLLAGCDQSSSDEKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVNDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            2299..2592
                     /gene="yicS"
                     /locus_tag="ACHKFK_001276"
     CDS             2299..2592
                     /gene="yicS"
                     /locus_tag="ACHKFK_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO9051656.1"
                     /translation="MKPTMLLMITIFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(2633..3481)
                     /gene="nepI"
                     /locus_tag="ACHKFK_001277"
                     /pseudo
     CDS             complement(2633..3481)
                     /gene="nepI"
                     /locus_tag="ACHKFK_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(3510..3962)
                     /gene="yicN"
                     /locus_tag="ACHKFK_001278"
     CDS             complement(3510..3962)
                     /gene="yicN"
                     /locus_tag="ACHKFK_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO9051657.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(4015..5349)
                     /gene="adeQ"
                     /locus_tag="ACHKFK_001279"
                     /gene_synonym="yicO"
     CDS             complement(4015..5349)
                     /gene="adeQ"
                     /locus_tag="ACHKFK_001279"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO9051658.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDSKVVFVTTCLIAGIGSIAMGVFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALSLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVATLFALKIILVD"
     gene            5524..7290
                     /gene="adeD"
                     /locus_tag="ACHKFK_001280"
     CDS             5524..7290
                     /gene="adeD"
                     /locus_tag="ACHKFK_001280"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO9051659.1"
                     /translation="MNNSINHKFHHISRTEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGHRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIDRQP
                     VSASDFALQFTPGKRYRVIEVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(7337..8727)
                     /gene="uhpT"
                     /locus_tag="ACHKFK_001281"
                     /pseudo
     CDS             complement(7337..8727)
                     /gene="uhpT"
                     /locus_tag="ACHKFK_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
     gene            complement(8865..10184)
                     /gene="uhpC"
                     /locus_tag="ACHKFK_001282"
     CDS             complement(8865..10184)
                     /gene="uhpC"
                     /locus_tag="ACHKFK_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO9051660.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSEMLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVIAIAAGISALLLLPFLNAQTPREA"
     gene            complement(10194..11696)
                     /gene="uhpB"
                     /locus_tag="ACHKFK_001283"
     CDS             complement(10194..11696)
                     /gene="uhpB"
                     /locus_tag="ACHKFK_001283"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO9051661.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLMQAVGLTHFSLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPDSGQHGFGLTGMRERVTALGGTLTISCLHGTRVSVSLPQRYV"
     gene            complement(11696..12286)
                     /gene="uhpA"
                     /locus_tag="ACHKFK_001284"
     CDS             complement(11696..12286)
                     /gene="uhpA"
                     /locus_tag="ACHKFK_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO9051662.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(12359..12649)
                     /gene="ilvN"
                     /locus_tag="ACHKFK_001285"
     CDS             complement(12359..12649)
                     /gene="ilvN"
                     /locus_tag="ACHKFK_001285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9051663.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQFDPTMFNKIAVFFQ"
     gene            complement(12653..14341)
                     /gene="ilvB"
                     /locus_tag="ACHKFK_001286"
     CDS             complement(12653..14341)
                     /gene="ilvB"
                     /locus_tag="ACHKFK_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO9051664.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPLVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYKQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(14447..14545)
                     /gene="ivbL"
                     /locus_tag="ACHKFK_001287"
     CDS             complement(14447..14545)
                     /gene="ivbL"
                     /locus_tag="ACHKFK_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO9051665.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            complement(14790..14864)
                     /gene="ysdE"
                     /locus_tag="ACHKFK_001288"
     CDS             complement(14790..14864)
                     /gene="ysdE"
                     /locus_tag="ACHKFK_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO9051666.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            14946..16130
                     /gene="emrD"
                     /locus_tag="ACHKFK_001289"
     CDS             14946..16130
                     /gene="emrD"
                     /locus_tag="ACHKFK_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO9051667.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLMVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLIPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(16138..16635)
                     /gene="yidF"
                     /locus_tag="ACHKFK_001290"
     CDS             complement(16138..16635)
                     /gene="yidF"
                     /locus_tag="ACHKFK_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO9051668.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQLGIRSVQLI
                     PHLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDTTLQKWCGSEKS
                     QPRRDCQACDWHRLCPHAREDKPDNVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(16632..16994)
                     /gene="yidG"
                     /locus_tag="ACHKFK_001291"
     CDS             complement(16632..16994)
                     /gene="yidG"
                     /locus_tag="ACHKFK_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO9051669.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(16984..17331)
                     /gene="yidH"
                     /locus_tag="ACHKFK_001292"
     CDS             complement(16984..17331)
                     /gene="yidH"
                     /locus_tag="ACHKFK_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO9051670.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(17391..18884)
                     /gene="yidJ"
                     /locus_tag="ACHKFK_001293"
     CDS             complement(17391..18884)
                     /gene="yidJ"
                     /locus_tag="ACHKFK_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9051671.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARADEPFLMVISYDEPHHPFTCPVEYLEKYTDFYYELGEKA
                     EDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVLVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIHFADVRSKMHDALLDYMDKIRDPFRSYQWNLR
                     PWRKDAQPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(18881..20596)
                     /gene="yidK"
                     /locus_tag="ACHKFK_001294"
     CDS             complement(18881..20596)
                     /gene="yidK"
                     /locus_tag="ACHKFK_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO9051672.1"
                     /translation="MNSLQILSFVGFTLLVAIITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQLSDG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNVEPQQLVTVGRKFGFFIAIVSVMV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLMIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAIISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            20763..21629
                     /gene="yidL"
                     /locus_tag="ACHKFK_001295"
     CDS             20763..21629
                     /gene="yidL"
                     /locus_tag="ACHKFK_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9051673.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNDEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEVYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMHAVGKKDPQRRQIEKLIATLHANLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDR"
     gene            complement(21719..23380)
                     /gene="yidE"
                     /locus_tag="ACHKFK_001296"
     CDS             complement(21719..23380)
                     /gene="yidE"
                     /locus_tag="ACHKFK_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO9051674.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGVGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMAMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESTRTNGGALIRTINIRVENPNLHN
                     LAIKDVPILNGDKVICSRLKREETLKVPSPETVIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVARVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASSD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFIHTLVDGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23578..24006)
                     /gene="ibpB"
                     /locus_tag="ACHKFK_001297"
     CDS             complement(23578..24006)
                     /gene="ibpB"
                     /locus_tag="ACHKFK_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO9051675.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(24118..24531)
                     /gene="ibpA"
                     /locus_tag="ACHKFK_001298"
     CDS             complement(24118..24531)
                     /gene="ibpA"
                     /locus_tag="ACHKFK_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO9051676.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(24525..24597)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            24837..25169
                     /gene="yidQ"
                     /locus_tag="ACHKFK_001299"
     CDS             24837..25169
                     /gene="yidQ"
                     /locus_tag="ACHKFK_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9051677.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVLDTILLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(25171..26385)
                     /gene="yidR"
                     /locus_tag="ACHKFK_001300"
     CDS             complement(25171..26385)
                     /gene="yidR"
                     /locus_tag="ACHKFK_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO9051678.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAYVGVVTVHPKSEKYVFIHGPENPDETWYYDFHHRRGVIVE
                     SGKVSNLDAMDITASYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSNEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPKDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EDGQLWITETDR"
     gene            26486..27586
                     /gene="cbrA"
                     /locus_tag="ACHKFK_001301"
     CDS             26486..27586
                     /gene="cbrA"
                     /locus_tag="ACHKFK_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO9051679.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRSVLLKQPEKLNTAYRRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(27547..28884)
                     /gene="dgoT"
                     /locus_tag="ACHKFK_001302"
     CDS             complement(27547..28884)
                     /gene="dgoT"
                     /locus_tag="ACHKFK_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051680.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFIGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(28959..30107)
                     /gene="dgoD"
                     /locus_tag="ACHKFK_001303"
     CDS             complement(28959..30107)
                     /gene="dgoD"
                     /locus_tag="ACHKFK_001303"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO9051681.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELSDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(30104..30721)
                     /gene="dgoA"
                     /locus_tag="ACHKFK_001304"
     CDS             complement(30104..30721)
                     /gene="dgoA"
                     /locus_tag="ACHKFK_001304"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO9051682.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(30705..31583)
                     /gene="dgoK"
                     /locus_tag="ACHKFK_001305"
     CDS             complement(30705..31583)
                     /gene="dgoK"
                     /locus_tag="ACHKFK_001305"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO9051683.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLDCRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMS
                     GELHHLLLNHSLIGAGLPPQENAADAFAAGLERGLNAPAVLPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(31580..32269)
                     /gene="dgoR"
                     /locus_tag="ACHKFK_001306"
     CDS             complement(31580..32269)
                     /gene="dgoR"
                     /locus_tag="ACHKFK_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO9051684.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            32548..33204
                     /locus_tag="ACHKFK_001307"
     CDS             32548..33204
                     /locus_tag="ACHKFK_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010377884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051685.1"
                     /translation="MKLNLKGFFKAASLCSLAFALTGCITWGLVSNTASHPHAEQWRS
                     DTIKGLSLAEDSNGTKGYVFVGESLDYLLTTGGDEVVNMLNDPAIHGERITVSDNAKF
                     ILSSSNKNFSGAITLYYDWNNEEDKALATQYGFICDTRRCTWMLDGLKGSIHQKNKKA
                     DYSNVMVFHQPFTVGFYEYKATDGVPRGLVNALLPVTLTLDIVTSPLQFLILCTTRNC
                     "
     gene            complement(33248..34060)
                     /gene="yidA"
                     /locus_tag="ACHKFK_001308"
     CDS             complement(33248..34060)
                     /gene="yidA"
                     /locus_tag="ACHKFK_001308"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO9051686.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(34175..34573)
                     /gene="yidB"
                     /locus_tag="ACHKFK_001309"
     CDS             complement(34175..34573)
                     /gene="yidB"
                     /locus_tag="ACHKFK_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO9051687.1"
                     /translation="MGLFDDVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQVESALGTNAVFDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            34691..34792
                     /locus_tag="ACHKFK_001310"
                     /pseudo
     CDS             34691..34792
                     /locus_tag="ACHKFK_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(34813..37227)
                     /gene="gyrB"
                     /locus_tag="ACHKFK_001311"
     CDS             complement(34813..37227)
                     /gene="gyrB"
                     /locus_tag="ACHKFK_001311"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO9051688.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYDILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTETDLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(37256..38329)
                     /gene="recF"
                     /locus_tag="ACHKFK_001312"
     CDS             complement(37256..38329)
                     /gene="recF"
                     /locus_tag="ACHKFK_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO9051689.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPQAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(38329..39429)
                     /gene="dnaN"
                     /locus_tag="ACHKFK_001313"
     CDS             complement(38329..39429)
                     /gene="dnaN"
                     /locus_tag="ACHKFK_001313"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO9051690.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(39434..40837)
                     /gene="dnaA"
                     /locus_tag="ACHKFK_001314"
     CDS             complement(39434..40837)
                     /gene="dnaA"
                     /locus_tag="ACHKFK_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO9051691.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            41134..41214
                     /locus_tag="ACHKFK_001315"
     CDS             41134..41214
                     /locus_tag="ACHKFK_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051692.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTRQPV"
     gene            41444..41584
                     /gene="rpmH"
                     /locus_tag="ACHKFK_001316"
     CDS             41444..41584
                     /gene="rpmH"
                     /locus_tag="ACHKFK_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO9051693.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            41601..41960
                     /gene="rnpA"
                     /locus_tag="ACHKFK_001317"
     CDS             41601..41960
                     /gene="rnpA"
                     /locus_tag="ACHKFK_001317"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO9051694.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            41924..42181
                     /gene="yidD"
                     /locus_tag="ACHKFK_001318"
     CDS             41924..42181
                     /gene="yidD"
                     /locus_tag="ACHKFK_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO9051695.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            42184..43830
                     /gene="yidC"
                     /locus_tag="ACHKFK_001319"
     CDS             42184..43830
                     /gene="yidC"
                     /locus_tag="ACHKFK_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO9051696.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            43936..45300
                     /gene="mnmE"
                     /locus_tag="ACHKFK_001320"
     CDS             43936..45300
                     /gene="mnmE"
                     /locus_tag="ACHKFK_001320"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO9051697.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVCAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            45542..45616
                     /gene="tnaC"
                     /locus_tag="ACHKFK_001321"
     CDS             45542..45616
                     /gene="tnaC"
                     /locus_tag="ACHKFK_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO9051698.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            45837..47252
                     /gene="tnaA"
                     /locus_tag="ACHKFK_001322"
     CDS             45837..47252
                     /gene="tnaA"
                     /locus_tag="ACHKFK_001322"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO9051699.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            47344..48591
                     /gene="tnaB"
                     /locus_tag="ACHKFK_001323"
                     /pseudo
     CDS             47344..48591
                     /gene="tnaB"
                     /locus_tag="ACHKFK_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
     gene            48723..49898
                     /gene="mdtL"
                     /locus_tag="ACHKFK_001324"
     CDS             48723..49898
                     /gene="mdtL"
                     /locus_tag="ACHKFK_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO9051700.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSALFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WTMATMGIAVLMLSLFILKETRPAAPTTSDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            49873..50832
                     /gene="yidZ"
                     /locus_tag="ACHKFK_001325"
     CDS             49873..50832
                     /gene="yidZ"
                     /locus_tag="ACHKFK_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO9051701.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMSVTPSAVSKSLA
                     KLRMWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNQLLDKPLHETPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHEAWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSRNPKIVWLRETIKNLYASMA"
     gene            50989..51738
                     /gene="yieE"
                     /locus_tag="ACHKFK_001326"
     CDS             50989..51738
                     /gene="yieE"
                     /locus_tag="ACHKFK_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO9051702.1"
                     /translation="MATHFARGILTEGHLISVRLPSLCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWELDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            51760..52326
                     /gene="yieF"
                     /locus_tag="ACHKFK_001327"
                     /gene_synonym="chrR"
     CDS             51760..52326
                     /gene="yieF"
                     /locus_tag="ACHKFK_001327"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO9051703.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEINALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQSTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(52380..53717)
                     /gene="adeP"
                     /locus_tag="ACHKFK_001328"
     CDS             complement(52380..53717)
                     /gene="adeP"
                     /locus_tag="ACHKFK_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO9051704.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMSVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            53884..54549
                     /gene="yieH"
                     /locus_tag="ACHKFK_001329"
     CDS             53884..54549
                     /gene="yieH"
                     /locus_tag="ACHKFK_001329"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO9051705.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFREFGIHVDLEEIFT
                     RFKGVKLYEIIDIISKEQGVTMVQADAEHVYRAEVARLFDTELEDIAGANELLASINV
                     PMCVVSNGPVSKMQHSLGKLNMLHYFPEKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSSAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
BASE COUNT        13520 a        14570 c        13662 g        12856 t
ORIGIN      
        1 aataaccacg acatcaacac ctgaacgaca gttttctatt tctgttctgg atgatgacaa
       61 caaagaaaat atacctaccg ataccgcacc ttgttttaca attggtattc ctgatgaata
      121 taccaaagcc atagagttat taaacattaa ataattaaat ttcatattga gtttttagtg
      181 agttttttta aataccatat gaataattgt tatatgtcat tatttcctcc attcactaaa
      241 actgattgat tactctactc cctcaccgtc atcggtagtg gtatccttaa cgtatattga
      301 tgaaaagaca aaacagtatg cagagggaaa aatgggttcc accagaaagg ggatgctgaa
      361 cgttctgatt gccgccgtgt tgtggggaag ttcaggggtc tgcgcgcaat acatcatgga
      421 gcaaagccag atgtcgtcgc agtttttgac tatgacgcgt ttgatattcg ccggtttgat
      481 tctgctgacg ctatcatttg ttcatggtga taaaatcttt tctattatta acaatcacaa
      541 agatgccatt agcctactga ttttttccgt ggttggcgcg ctaaccgtac agctcacttt
      601 tttgctaacc atcgaaaaat cgaacgcagc cacggcaacg gtgctgcaat tcctctcacc
      661 gactattatc gtcgcctggt tctcactggt gcgtaaatcc cgcccgggca ttctggtttt
      721 ctgcgctatt ttgacatcgc tgatcgggac ttttttattg gtgacacacg gtaatccgac
      781 atcattatcg atctctcctg ccgcgttatt ctggggcatt gcctcggcgt ttgctgctgc
      841 attctatacc acctatccct caacgctgat tgcccgctat ggcacgttac cggtcgtcgg
      901 ctggagtatg ctgattggcg gtctgatcct gctgcccttt tatgccagac aagggacaaa
      961 ctttgtcgtt aacggcagtt tgattctggc gtttttttat ttggtggtca ttggtacgtc
     1021 cctgacattt agtctgtacc tgaaaggagc acaattaatt ggcggtccaa aagccagcat
     1081 tttaagctgt gcagaaccat taagtagcgc gctgctctct ttgctgttgc tgggaatcac
     1141 cttcacattg ccggactggc tgggaacgct gctgattctg tcatcggtga ttttgatttc
     1201 aatggattcc cgccgccgcg ccagaaaaat aaatcgtccg gcgcggcatg agtgagaatt
     1261 accagccagg caccgcgcca ccgttaaaga tggtttctgc cgctttagcg acttcgggtg
     1321 attgataaga ttgcagaaat tccttcacgt tttctgcatt cttattatct tcccgtgcca
     1381 ccagaatatt cacatacggc gaatttttat cttcaataaa tacgctgtcg ttaaccggag
     1441 aaagtcctgt ttgctggatg taagtggtgc tgataatcgc tacatccact ttcgggtcgt
     1501 ccagtacacg tggcaactgt gccccttcca gctccattat ctgcaaatgg cgtggattat
     1561 cggttatatc caacgcggta ggtaataacc cttttccttc tttcagagta attagcttct
     1621 ctttttgcag cagtaaaagc gcgcgaccaa ggttagtcgg gtcattgggg atcgccactg
     1681 tcgcgccttc ttttatttgc gccacggttt tgattttttt ggaataaccc gccatgggga
     1741 agacaaacgt atttcccacc gctaccagtt tataaccatg cgcctgatta tcttgttcaa
     1801 gaaagggtcg atgctggaag acgttcgcat caagctcgcc atgatttgtc gcatcgttcg
     1861 gtagcaacga accgctaaat cctaccagtt caacatcgag gccatatttc tcttttgcta
     1921 cctttttagc gacttccgcg acatcttgtt ctgcgccatt aataacgcca actttaatat
     1981 gcttttcatc gctgctgctc tggtcgcaac ctgccagcag aactcctgcc agtaataatg
     2041 cggcccccgc ccgtagatga tgtgttgtca gtttcatttt tttatccttt tattaatgat
     2101 tacctgacta tagcggtaag gtcgctgcag tttaaaaaac gaaacgctat cgataagaat
     2161 aaaaaggaat aaaagtggaa tataaagtct ggcacttctt actgaccacg caaacacctt
     2221 tcgtacagca tgatgagagc ggcaaaagca aactacactt atgcttcatt cgatgcacat
     2281 ttgcagaagg ttgccgctat gaagccaacg atgctactca tgattaccat attcctgatt
     2341 tttccagcca tttctcaggc cgagtcgcca ttcagttctc tacagtcggc aaaagagaaa
     2401 accacggtat tgcaagattt acgtaaaatt tgcacgccac aggcgtcatt atcagatgaa
     2461 gcgtgggaaa agttaatgtt gtcagatgaa aacaataaac aacatatacg cgaggccatc
     2521 gtggcgatgg aacgtaataa tcagagcaat tattgggaag cgttgggtaa ggtagaatgc
     2581 ccggatatgt aagaacggca acaaccactg accaacgctt ctttacctga tatcaggatt
     2641 tcttcatttt cacctttgca gtcaccaaca atgcagtcag caacatcaat gtgccggaca
     2701 acatcagcgg cgaagtcaga ccaatgttat ccaacgcata accgccgatt gccgcaccgc
     2761 aggtgttagc aagctgaata acggccacct gaatagatcc agccttttct gcctgatcgg
     2821 ccaatgagcg agtgatccac gttgaccaac caacgggaat taaagcaaag gtcagccccc
     2881 agataatcgc cacaccagta gcaacaattt tatcgcttcc ccacagcgtc agtaccagcg
     2941 cgctcagggc gagcacaaac ggtgcgcccg ctaaggccag tttcaccgaa cgtttgagaa
     3001 taaacgacga aagcgatgtc ccgacaaagc tggcgatacc aaaactcagc agcaccaggg
     3061 ttaagccatc aacaccgaat cccgccagat tcatatacac tgggcgaata taagtgaaaa
     3121 aagcaaactg accggcaaag gacataaaga tggcgatcat cccggccatc actcccggac
     3181 gttgtaataa gcggaaagta ttttgtttct gatgcgaagg ttcacctggc agcgaaggca
     3241 gtgatttgat aatccagaaa atatacagca cgcccatcgc cgccgccgca ttaaagacat
     3301 tgcgccaacc gataagctcg cctaaaaaac tgcccaacgg cgcggcaatc accagcgcaa
     3361 tagaaaccgc gccgaagatc accgacagcg ccttcggcac cgtacgcggc ggtaccagac
     3421 gcatggtcag cgacgccgac atcgcccaga acccgcccag cgccagcccc agacaggcgc
     3481 gatctgtagg ctgataagac gaagcggttt taatgcagcc agtgaacgcc ctcggcaggc
     3541 tgaaagaacg cgttatagcg catctgaaaa gcattgatat cctgcatttc aggttcggtt
     3601 tcgcgcagaa aacccagcgc cagcctgacc tgagcatcag taatcggcgc ggcaaggcgg
     3661 gctttttgta aaatccgatg ccattgcagc aggttctgtt cgctaccatc gacaatgaca
     3721 atttgccaga tgagcaacac ctgcgcggcg ttttgcaggt gcgactcatc cgggcgatgc
     3781 aaataacgta aaaattcgtc accggctgac tttcccattt gatcgaccat cgactccacg
     3841 ggtacgacat cgatgttcag ccgatcgctg agacggcgaa tcgcttttct ggccccggat
     3901 gtcagcaccc gaaaaccaac cacaaacact accgccagcg tggcgagcat tatccagatc
     3961 atgagttctc ctgcgtaatc agaactcatg atatctggaa accctcgcca gttactaatc
     4021 caccagaata atcttcagtg caaacagagt tgcgaccacc accacacaca ggttcagatc
     4081 gcgccagcgc ccggtgcaca ctttcatgat gcagtacgac ataaagccta gtgcaatccc
     4141 ttcggtgatc gagaaagtaa agggcatcat caccgtggta ataaacgcag gcaccgattc
     4201 ggtaaaatca tcccagttaa cgcgcgccag gcttgaagtc atcagcacgc caacaaagat
     4261 taacgctccg gcggttgcgt aaggaggaac catcgccacc agcggtgaga agaacataac
     4321 taacaggaac ataacgccaa ctacgacagc cgtcagcccc gtgcgaccac cgacagccac
     4381 acctgaagta ctttcaatat aggcggtaac agacgaggta ccgataaacg cacccgccac
     4441 cgaactgacg ctatcaacat acagcgcctt attcatattg gggaatttac cgttactatc
     4501 tattaagcca gctttatcag tcacaccaat taatgtccct gatgaatcaa ataagttgat
     4561 cagcataaag gagaaaatga taccggcgag ttcaagtgat aacgcaccgc tcaaatccac
     4621 ttcaccaatg acgccgctaa tatcaggcgg aatggaatag acgccgctaa aatgaacatc
     4681 accgaaaaac aatccacagc aagacgtcac cacaatagaa acaagcaccg cggcatgaaa
     4741 atgacgtgat gacaacacgg ttataataaa aaaccctaaa atacctaaca acacgccgtg
     4801 agaacttaaa tcgccaatca tcaccagggt gtctttattg gcgacaataa cgccagtatt
     4861 ttttaatccc attaaggcga taaataatcc gattccgctg gtgataccaa tacgtaaact
     4921 taatggaatg ttggagatca tccagtaccg gatacgaaac agcgtgagca aaaatagccc
     4981 aactgcgccc cagaatatcg cgcccatccc ggtctgccag gagataccca tcgcccccac
     5041 gaccacgaag gcaaagaagg cgttcaggcc cattgccgga gccagtgcca cgggtaagtt
     5101 agcaaatacc cccatcgcaa tactgccgat accggcaatc aaacaggtgg taacaaacac
     5161 cacttttgag tccatttgtg ccgcgccgag gatttgcggg ttcacaaaaa cgatgtacac
     5221 catggttaaa aaagtggtca tccccgcaat caattctgtg cggacggtgg tcccatgctg
     5281 aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat cggtattgtc
     5341 attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta aaacaaatga
     5401 caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat attctttaat
     5461 aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca aaatgagaaa
     5521 aatatgaata attctattaa ccataaattt catcacatta gccggactga ataccaggaa
     5581 ttgttagccg tttcccgtgg agacgctgtt gccgattata ttattgataa tgtctctatt
     5641 ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg acgttacatt
     5701 gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga tgcccgcggc
     5761 gcaacggcgg tgccagggtt tattgatgct cacctgcata ttgaatccag catgatgacg
     5821 ccggtcacct ttgaaactgc taccctgccg cgcggcctga cgaccgttat ttgcgaccct
     5881 catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg ctgtgccgaa
     5941 caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct ggaaggctgc
     6001 gatgttaatg gtgccagttt tacccttgaa cagatgctcg cctggcggga ccatccgcag
     6061 gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca gaatgcgctg
     6121 ctcgataaac tggacgcatt tcgccacctg acactggacg gtcactgccc gggtttgggt
     6181 ggtaaagaac ttaacgccta tattgctgcg ggtattgaaa actgccacga aagttatcag
     6241 ttggaagaag gacaccggaa attgcaactc ggcatgtcat tgatgatccg tgaagggtcc
     6301 gcagcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag cccgcaatgc
     6361 atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca catcgatgcc
     6421 ttaattcgcc gcctgatcga acaacacaat gtaccgctgc atgtggcata tcgcgtagcc
     6481 agctggtcga cggcgcgcca ttttggtctg aatcacctcg gcttactggc acccggcaag
     6541 caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca ggtactggtg
     6601 aaaggcgagc cgatcgatgc acaaacctta caggcggaag agtcggcgag actggcacaa
     6661 tccgccccgc catatggcaa taccattgat cgccagccag tttccgccag cgactttgcc
     6721 ctgcaattta cccccggaaa acgctatcgc gttattgagg taatccataa cgaactgatc
     6781 acgcactccc gctccagcgt ctacagcgaa aacggttttg atcgcgatga tgtgtgcttt
     6841 attgccgttc ttgagcgtta cggacaacga ctggctccgg cctgtggttt gctcggcggc
     6901 tttggcctga atgaaggtgc gctggcggcg acggtcagcc atgacagcca taatattgtg
     6961 gtgatcggtc gtagcgcaga agagatggcg ctggcggtca atcaggtgat tcaggatggc
     7021 ggcgggctgt gcgtggtccg taacggccag gtacaaagtc atctaccgtt gcctattgcc
     7081 gggctaatga gcaccgacac ggcgcagtca ctggcggaac aaattgacgc cctgaaagcc
     7141 gccgcccgtg aatgcggtcc gttaccggat gagccgttta ttcagatggc gtttctttcc
     7201 ctgccagtga tccccgcgct gaaactaacc agtcaggggc tgtttgatgg cgagaagttt
     7261 gccttcacta cgctggaagt cacggaataa taaaaaaaag cccggcgtca tgccgggcaa
     7321 aagtcaccag ttacgtttat gccactgtca actgctgaat tttttctcgc ggcggatttt
     7381 gcgttcttcc atcaccgcta ctatcgccat caggcagata caaccaatag cggcgatatc
     7441 cagcgcggcg aaggtgcctg cccagccggt aaggccaaat accggcgtcc catcggcaat
     7501 cattcccaga cctaacttgg caaagctgtc accaatcagg taagcgaagg tgcctttaat
     7561 accatcggca gcgccaatcg cttttttagg tacaaagcca acagcagcca caccaatcaa
     7621 caattgcggg ccaaagacca ggaaacccaa cgcaaagaga gaagccaggt agatatattc
     7681 gttgctggcg tgttgataca caccgagcgt ggcgataatc agcgccagcg cgatacaggc
     7741 caccaggcca cggcgaccgt tcgccaggtc agagagccag ccccacagca gcgtaccgac
     7801 cagcgcacca gcttcaaaca gcgtaaagcc ctgaattgcc accgctttag agagtttcag
     7861 ttcctggaac gcatacacgg ttgaccactg gtcgatacca atacgcacca catagaggaa
     7921 aatgttggca aagcacagca gccagatcac tttgtttttc agcacatact caacaaagat
     7981 ctgccacttg gtcatatcgg tagattctgt ctctttatcc tcttcactga tctcctcgcc
     8041 aaacagctct tcagctttgc cgaggccata agattccggg gagtcgctgc cgtaacgcag
     8101 accgataaaa ccaacaatca gcgcgataat cgacgggaaa ataaacatgc cgatgacatg
     8161 accatcgaac aggtaatttg cgccgaacag cgccacacct gctgcgcctg caccaccaag
     8221 gttgtgagaa atgttccaga aaccgaggaa tgtcccgcgt ttacgacgcg gcgtccattt
     8281 ggtgatggtg gagtaactgc acgaaccgcc ggtactctgg aaaaagccgc ttaaggcgta
     8341 gaaggcaatc atcaggaaca ggctaaccga gccgctgccc atactggcac tgaagcccag
     8401 catacaaatg gcagacagga tcagcatgaa cggcaggaat tgtttggtgt ttttgccgtc
     8461 ggcgtagtaa gaaaccagcg ttttacccac gccataagtg atggagaagc ccaggccgat
     8521 catccccagc tgcgtcatgc tcaacccgta ggtcgagatc atgtcgttct gcgcgatgtt
     8581 aaagttcttg cgaatcaggt acatcgtaag gtagccgata aagaccacca ggtaagattg
     8641 catgaacggt ttgaaccaca ttttgcgccg cacttcgagc ggaaggtcca gggtcggctt
     8701 gcgaacctgg tttaagaaag ccagcatggg ttactcctga aatgaatacc tgcgtgaggc
     8761 atgcattgta aaaatcagca gcaaatcagg cctgagatag cgtccaggca aaacctggaa
     8821 aatttcttag ttttgccgga tatgaagtga aaaggtgaga tgcatcacgc ttctcgcggt
     8881 gtctgggcgt tcaaaaaggg cagtaatagc agtgcggaaa tcccggcggc gatggcgata
     8941 accacaaaaa atccgctcca gtgccaggta tcgagtactt tcgccagcgg ccaaccagca
     9001 agcgacgcac caagataagc aaacaagccg acaaatcccg tcgccgcccc tgccgcctct
     9061 ttgtgggaac actctgccgc cgccatgccg attaacatct gcgggccaaa gacaaaaaaa
     9121 ccaatggtga agaagcaggt tgcctgcatc acgtagctgg caaatggcat cagccacagc
     9181 gagccgactg aaagcaaaat tccggcggcg aaaatcaaat tcatcggccc gcggttgccg
     9241 ttaaacagtt tgtccgaacc ccaacccgct accagcgcac cgataaatcc gcccagttca
     9301 aacattgtca ctgccgtatt cgccgtgacc agatcgacgc ccagcatctc ggacatatac
     9361 aaattgcccc agtcgttgat cgccgcccga accacataga ccagcacata gcaaaacgaa
     9421 agcagccaga tatacggatt cagcaacaca tatttggtga ggatctcttt acgcgtcagc
     9481 cctgcccctt cttgttgttg agcaatctcc agtgcgtcgt gccgccattc accaaccgcc
     9541 ggtaaaccta acgcctgcgg gcgatcgcgc agccgccagc agagaaaaat ccccacgact
     9601 atcgccatac aacccgcaat catcatcccg gcacgccagc cgtaatgcag cgcgctcgct
     9661 gccatcacaa tgggaatgag tgcgccgccg acgttatgcg ccgtgttcca taatgcccac
     9721 caaccgccgc gctcggtacg agaataccag gcggttaaca gacgcgcaca caccggtgaa
     9781 ccccagccct ggaaaaaggc gttcaacacc caaagcacgg caaacgccca tagcgacgtc
     9841 gagaagccaa acagaatgtt gataatgccc gtggcaataa gccctatccc cataaaataa
     9901 cgggcatttg agcgatcgct gacaatgccg gagacaaact tcgacacgcc gtaggtaatg
     9961 taaaacaggg tcgctaacag gccaatatcg ctacggctga gcacgccgtt agcaaggatt
    10021 tctggtacgg cggcgttaaa acttttacgc gtgaagtaaa acagcgcgta acccagccag
    10081 atggtcagca gaatatgccg acgccagtag cgatagcggg catcaatttc atgtttatca
    10141 gtcattaatg gcgcatcggc gggcgctttc agaaacggca acatcatcga accttagaca
    10201 taacgttgag gtagagaaac gctgacacgc gtgccgtgca gacaggaaat ggtcaatgtg
    10261 ccacccagcg ccgttacgcg ctcgcgcatt ccggtaaggc caaaaccgtg ttgcccggaa
    10321 tctggcggta aaccgctgcc atcgtcttca atcaccagca tcaaccgttc atcctgctgc
    10381 cagccttgca gggtgaccgc gctggcatca gcatgtttca caatgttgtt cagcccttcc
    10441 tggcagacac gaaacagcgt cactcgctgg ttttcgctta acgctgattc atcgattcgc
    10501 cattcgagat ggctgacaat gccgcgccct tccagctcca tttcccgcat cagtgagcgg
    10561 atggcctgct ccagggtgag atcatccaac tggcgcggac gtaaccgacc taacaaacgg
    10621 cgcaccgcgt cgtaaacgcc cagcgatagt tgttcgatga gctgcccgct ctgtttcacg
    10681 ctggcgttat ctgccgccag ccgctgaaca atgcccgctt gagttcgaat agcagtgatg
    10741 gtctgaccga tatcatcgtg cagctcacgc gccacatcac ggcgcacgct ctcttcggtt
    10801 tccagcaacc gttcagcgag atgctggttg cgcgccagtt ccttttgcag tgactggtta
    10861 agttcacgca accgctggat gcctgcgccc agtaacaacc ctgtcagact ttgcaccagc
    10921 agcgagagca ataaatccac cggatgatcg cgccaggttt ggctggcgat cagcgcgatg
    10981 gcgttcatca atgtcgcaat cagcgctcct tgccaaccat agtgccaggc cagcgcgata
    11041 atcggcagcg ccagacagaa tggcgtaaag cgcgacagtt cgtctggcaa tcccaactgg
    11101 agccagagac tgataacaaa cagcagcaag taccagacca gatgtcgccc gcgccagttg
    11161 attggctgag aaaccagtga cgggccgagc ggcagccagg tgttattggc gagatagtgc
    11221 cagaacacca ggcaaatggg ggccagcgtc aggccgccag ttaaagtcag cagcagcgca
    11281 ttccacgact ctttgccgtg ccaaagccag ggcagcgact gcaacaacgc cgccgccgtt
    11341 aacgccgcac cctgtaacag caaggtgcgc cagtcacgtt gatggcgata gcgcgagatc
    11401 agcgccacgg gcaataacgt cagtaaacta ccgatcatca ataaggagaa atgggttaaa
    11461 ccgaccgcct gcattagcca gtaaatcagc agccactccg cacccagtag caccggccag
    11521 tatccgcgcg ggcattgcag cattagccct agccgcaggc caaacggaaa taacagcacc
    11581 gccatatcag ggcgctcaac caggtgcagg ctgatactcc acaggcaaaa ccatgcggca
    11641 gagaagataa aaaagcaggc aataacggta attaagcggg agaacaacgt cttcatcacc
    11701 agccatcaaa catgcggcgc gccagttcaa cgtcgttact gacgcccagt ttttccatca
    11761 gattggcgcg atggacgtgt accgttttcg gcgacaagcc cagttcggcg gcaatctctt
    11821 tcaccgccat tccttgcgcc agtttttctg ccacctggcg ttcgcgtttg gttagcgggt
    11881 cctggcgacc ggatgccagt ttaatggcaa tatccggcgt cagataacag ccgccagtgg
    11941 caaccgtatg caccgcagca atgagttcat ccgggctaca gcgtttggag agaaagccgc
    12001 gcgcccccgc gttaagcgcc tgctcaacca gcgccggact gtcatgaacg gagagcataa
    12061 ttgtcgccat gcctttcggc agctggctta gaagctccag accggagata tcaggcatgg
    12121 agatatcgca aatacacacc tgcacaccgc gccccggcag ccccgccagc gcctcgcgcc
    12181 ccgaaccaaa ctcggcaact acctgcaaat caggttccag ccccagcagc tgcgcaaagc
    12241 cggagcggac gataaggtga tcgtctataa gggcaacggt gatcatgatc ttgtcctggc
    12301 gggtaataaa acgcgcttac cttaacgata agcgcgatgt tgttcaagcc ttgagcggtt
    12361 actgaaaaaa caccgcaatc ttgttaaaca tggtcggatc gaactgatta cgctgcactt
    12421 tcaccacatc ttccagcttg tcgatttggc ttatcatctg ctccagacgc tggtcgtcat
    12481 tgaccagtag ccagatgtgg cttttgtcgc tgtcctgaat cggcaggcag agaatgcctt
    12541 caacgttaaa agcgcgacgg gcaaaaaggc cacaaacgtg ggtcattaca cccggatggt
    12601 tgcgaacggt gagttccaga atcacgttat catgagttgt gttttgcatg gcttattccc
    12661 ccaccatttc agtattcgcc gcacctggcg gcaccatcgg gtacactttt tcttcggcat
    12721 caatgcgcac atggatcagc gccgggccag ggcgattgat gatttcctgc aatgcagcct
    12781 gcggatcggc ttcgttattc aaatcacagg tttcgaggcc gaatccggcg gcaatctgca
    12841 taaagttgat tttgcccgga taggtagcgg caaaaacgcc ctgcttgtag aacagactct
    12901 gttgctgatg caccagcccc agcgcttcgt tgttcatcaa aatgatttta acatccagct
    12961 gattttcact ggcggtcgcc atctcctgaa tattcatcat caggctgccg tcgccggaga
    13021 agcacaacac tttgcggtcc gggttcgcca gcgccgcgcc aatcgccgca ggcaggccaa
    13081 aacccatcgt gcccagcccg ccggaggtca gccactggcg tgggcgattg agcggatagg
    13141 cttgcgcggt ccacatctga tgctgaccca cgtcggtggt gataatcgca ttgtcatcga
    13201 cacaggcggc aacggcgttg atcaggccgt aatggcttaa tggatcgcac gctttcggga
    13261 ttggacacgg gaattcacgc tgcaaatccg ccaccaactg atgccactct gcacgcggtt
    13321 gcgcttccac cagcgggatc aactgcgcca gcacgtcatc aacatccgcc tgaattgcca
    13381 catgcggttg cttgattttg cccagctctg cgcggtcgat atcgacatga ataattttgg
    13441 cattcggaca gaactgctcg gttttgccaa tcgcccggtc atcaaaacgc gcaccgagta
    13501 caatcagcag atccgcctcc tgcaaaatat agttggtgct gcgcacaccg tgcatcccca
    13561 gcatacccag cgacagcgga tgcgcttttg gcaacatacc cagcgccatt aaagtcatgg
    13621 tggtaggcag ttgcgctttc tccgccagtt cacgcactcg cgcaggcgcg ttgatcacac
    13681 caccacccag ataaagcacc gggcgtttgg cggcgttaat catcgccgcc gcgtcacgaa
    13741 tactttcttc gctaaaggcg ggtgcggcgg ctttttctgc catagcgggt tgtgtttcaa
    13801 tctcaaaaac tgccgtttgc acatccttag gaatgtctat ccacaccggg ccagggcggc
    13861 ctgattgcgc aatacggaag gcatcgctca tgacctgcgg gagttcttcg atatgtctga
    13921 ccagatagtt gtgtttggtg atggggatag agatgccgta ggtgtcgact tcctggaagg
    13981 cgtcggtgcc gatcatcgag gcgggaacct gaccagtgat gcaaatcagc gggatggagt
    14041 ccagcctcgc atcggcaatg gcggtcacca ggttagtcgc acccggtcca ctacaggcca
    14101 tacagaccgc cggtttaccg tcggtgcgcg ccattccctg agcgataaac cccgcgccct
    14161 gttcatggcg agccagaata tggcggattt gcgtgctttg gcttaaggca tcgtaaacag
    14221 gcaggataga accgcccgga atacccgtca caatcttaat gccctgctgt tccaggaaat
    14281 gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga tgttgtgccc gaacttgcca
    14341 tgctccagtc cttttcttct gggccgactt tccgggaggt gcttaaacga aaaaccccgc
    14401 ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc cagtccctac ggcgcattgc
    14461 cgacgaccac caccacacgc acgacgacca ctgcggcaga tggcgcagtt ggtagtagtt
    14521 ttgcgttgaa catggaagta gtcattaggg accttgttgg ttttgtgttt aacaatattt
    14581 atacaagcac agctttacag gggagacaat ggaaaatttt tcttccggat aaaaatacgt
    14641 ggcagatcac attttgaact gaaactcagg caataaagca tcatcacctg aaagacggtc
    14701 agattgttga cagagtgcgc cttattcatg tcgaatacag cgtaaacacc ttaccctacc
    14761 aaccgcacca tcactgttcc cggcaacatt tattgccgct tttgtttaca tattctgccg
    14821 ctaaacaatt ccccattcct ggcgtatatc tggctaacat tcatcaatgt gatagattcc
    14881 tctcccgcat ttatgggaat gcgtagtgac ttattctaat tatttttata aaagcatccg
    14941 tgataatgaa aaggcataga aacgtcaatt tgttattgat gttggtatta ctcgtggccg
    15001 tcggtcagat ggcgcaaacc atttatattc cagctattgc cgatatggcg cgcgatctca
    15061 atgtccgtga aggggcggtg cagagcgtaa tgggcgctta tctgctgact tacggtgtct
    15121 cacagctgtt ttatggcccg atttccgacc gcgtgggtcg ccgaccggtg atcctcgtcg
    15181 gaatgtccat ttttatgctg gcaacgctgg tcgcggtcac gacctccagt ttgatggtat
    15241 tgattgccgc cagcgcgatg caggggatgg gcaccggcgt tggcggcgta atggcgcgta
    15301 ctttgccgcg cgatttatat gaacggacac agttgcgcca cgctaacagc ctgttaaaca
    15361 tggggattct tgtcagtccg ttgctcgcac cgctaatcgg cggtctgctg gatacgatgt
    15421 ggaactggcg cgcctgttat ctctttttgt tggtactttg tgccggtgtg actttcagta
    15481 tggcccgctg gatgccggaa acgcgtccgg tcgacgcacc gcgcacgcgc ctgcttacca
    15541 gttataaaac gcttttcggt aacagcggtt ttaactgtta tttgctgatg ctgattggcg
    15601 gtctggccgg aattgccgcc tttgaagcct gctccggcgt gctgatgggc gcggtgttag
    15661 ggctgagcag tatgacggtc agtattttgt ttattctgcc gattccggcg gcattttttg
    15721 gcgcatggtt tgccggacgt cccaataaac gcttctcaac gttgatgtgg cagtcggtta
    15781 tctgttgcct gctggctggc ttgctgatgt ggatccccga ctggtttggc gtgatgaatg
    15841 tctggacgct gctcattccc gccgcgctgt tctttttcgg tgccgggatg ctgtttccac
    15901 tggctaccag cggcgcgatg gagccgttcc ccttcctggc gggcaccgct ggcgcgctgg
    15961 tcggcggcct gcaaaacatt ggttccggcg tgctggcgtc gctctctgcg atgttgccgc
    16021 aaaccggtca gggcagcctg ggattgttga tgaccttaat gggattgttg atcgtgctgt
    16081 gctggctacc gctggcgacg cggatgtcgc atcaggggca gcctgtttaa gctcacgtca
    16141 ccgcagcatc gtcatcagct ccatgggaga acgatgctgc tttatcagat cacgcatcac
    16201 ccgcatatgc ggcgcggagt aagaataaaa cgcctgatag cccgcacaaa gcacgttgtc
    16261 tggtttatct tcccgagcgt gcgggcacag ccgatgccag tcgcacgcct ggcagtcacg
    16321 gcgcggttgc gacttttcgc tgccacacca tttttgtaag gtggtgtcaa aaagttcaat
    16381 cgaaatacga ctgatatctg cccgcaccca acactcgaac agggcaatta aaaatttccc
    16441 ccacagctct gctgtcaccg aagcggccgt cagatcgccc cggtcatcaa attcaagatg
    16501 gggaataagc tgtactgagc ggatgcccag ctgtttgaaa ttgtgataaa aatcggcagg
    16561 ttgcagagca tccttatact ctacaaccag tttatgcctg tcctcttcag catcaatgac
    16621 ctgacttcct gtcatgcgac tctctcaata aatacgataa gttgatgtat atgcgttaca
    16681 gcaaacagta ttgcgagaga taacaccgcg agggagatca aaaatttgtc acggactacg
    16741 tgaaattggg aaaaatcgct attcgtgaca tccattaaat tgcgattacg ggtgtagtgc
    16801 cagaggatca gcgccacgat ggcgaggatg ccaatcgaaa tccagaataa catgcccgcc
    16861 tggtgccagt tgtgtttgat agccaacgcc atcaacgcgc cgtagcccag catggtacga
    16921 aaccacgcca gtgatgtgcg ttccggctgc agccctgggt cggcaatgcg tctggctttg
    16981 cggctatccg gcatacaaca ccagtcccat gacgatcact gccacgacca tcaatattaa
    17041 actgatgatt aataagctgt tggtataggg caggtcctct ttcaggcgca tcgctttttc
    17101 attgcgtaac cagcgcaagt agccatacat cgctaacccg ccggaaaaca gacacaacaa
    17161 caacgccagc agttcgcgga tgacgggcgt ggcgaaatct ggcgcaagtt gatcaagccc
    17221 aacccccgcc gccagaaatc ctagtgcagt acggatccac gccagaaaag tacgctcatt
    17281 agccagcgag aagcggtaat cgggcgcttc tccgaggcgg gaaattttca ttgttgctcc
    17341 ttgtaaactg ttcctcgccg aaaaattagg ccaggaacag caataatgca tcagaacttc
    17401 tgttttttct cttccacctt caccccttgt gtcggtaaac cggtgtcata gtcgcgtacc
    17461 actggagaat aaccatcctg tgggcgtgga cgaaacgccc ccatccagcg cggttgtgca
    17521 tctttgcgcc acggacgcag gttccattga taactgcgga acggatcgcg aattttgtcc
    17581 atgtaatcca ataaggcgtc atgcattttg ctgcgaacgt cggcaaaatg gatatcatcg
    17641 atcaggttat gcatttcatt tgggtcatta cggcgatcgt aaagttcatc actggtgaag
    17701 aggttgagca ccagtttaaa gtcatccgtt acccagcaac gcaccggaat aaaaccgcca
    17761 aagctgtcat gctcaatctc gtagcgatta aattccacca acacgccacg tggttctttt
    17821 acggcaagga tattttcccc cggcagaatc tctggttttt caatatctgc cagcgccatc
    17881 attgtcggca gtaagtcaat atgactgact ggcgtatcga cctgtcgccg ctccccttgc
    17941 ggcgaacgga tgatcagcgg aatgcgggtg atgtcgtcat acatcgccgc tcctttactg
    18001 atcagcttat gtgcgcccat catttcgccg tgatcggagg tataaataac ccacgtattt
    18061 tcacgttgct ctggcgttaa ggcattgatg acccgtccga tttggtcatc aacaaagtca
    18121 ttgcaggcaa aatagagcgg atggtgataa agcccgtcat caccgactgg cgatggcatc
    18181 gcctgcgccc ataagcggtg atgttccggt ttgttcgcca ggtcatcctc tgctttctca
    18241 cccagttcgt agtaaaaatc agtgtatttc tctaaatact ccaccggaca ggtgaacggg
    18301 tgatgcggct catcatacga aatcaccatc aggaaaggtt cgtcggcgcg cgcgggctgt
    18361 tgcagaaaat ccaccgcccg attgctgatg cggtgcgccc aggtgaaggt ttcgtcaata
    18421 tggttcgcct gtaaatcctc aacgctgttt aggccattgc gccacaggct aatctctttc
    18481 tccgtcagtt cactaagata gttcgctcca tcgaaccagt aatcagcgtc ccactccggc
    18541 ggacactcgc cagtgccgaa atagtcatgt ccatcgagat gccatttgcc gatgtaacag
    18601 gtgtgatagc ccgcatcctt aaagtagcgc cccatggtgg aaatgttttt gcccggcgcg
    18661 acgttgttgg tccacgggcc ggactggtta gcgtagatac cggtgaatag tccggcgcgt
    18721 gcaggcgtac aaaccggtga acaagtgtag gcggaattaa agcgaatacc ttccgccgcc
    18781 agactatcaa tattttgcgt attcagcggt ttaccgctat agcaaccgac catattggtg
    18841 gcctgggtat cggtcatgat gaacagaaaa ttggggcgtt tcataggcta tccttcgcat
    18901 cgggagtaaa ggttacggct gggtcgtgac gatgccgcca gctgtcaaaa atcaggtaga
    18961 caatcactac ggcggcgatg aaataactga ttatcgctaa ccagcgcgta ccgtagccac
    19021 cgaattcagc cagcccggca tagacgccaa tcatcgcgaa caggatacca attgacgcaa
    19081 tcttgacgtt tttccacggt ttcatgtcca ccgcaaacgc atctttgaag gtgaacggcg
    19141 tggcgcgcgg tttgataaaa ccgatcatca gcatcacgac cacgttgatg cagaacgtac
    19201 aggccagcac atagaggaaa tggaagtcga acttcaccag atagttgatg gtgatgtagc
    19261 tgattatgcc aatccccatc gccacttttg ccgccagcgc cggaatgcgc gggaagaaaa
    19321 agcccataat gatgatggta accagcggca cgttgtaaat accgttgagc tgtttcatcc
    19381 agctatacag cccctgcggc gcgttggcga tccacggcgc gaccatcacc gaaacgatgg
    19441 cgataaagaa accaaatttg cgcccgacgg tgaccagctg ttgcggctcg acattctggt
    19501 taatgatgcg ccggtaaata cccatactga ataacgtact ggcgctattc agaaagccgt
    19561 tgaaggtact gatcaccgca ccaaataaca ccgcaccgaa gaagcccacc agtggcaccg
    19621 gcagaacgtt attgaccagc gtcgggtagg ccatgtcggc tttcggtaaa tcctgataca
    19681 gatgaaacgc aatcaaccct ggcagcacca gtaccagcgg gtcgagcatt ttcagcaccg
    19741 ccgttaacag cgcccccttt tgcccttccg ccaggctttt tgacgccagc gtgcgttgca
    19801 cgatgccctg atttgtacac cagtaaaagg tgttcaccag aatcaaaccg gtaaatgccg
    19861 cgccaatcgg caagggatcg gtcgggccgc cgactgagtt taatttctcg gcgtgaacgg
    19921 tggtgagttg ctcaatgccc tgcataaagc tgcccttgcc catcgcgatc aggccaaata
    19981 ccggcaccat caacccgcca ataaccagcc caataccgtt aatggagtcc gccactgcca
    20041 ttgcgcgcag tccgccgatc accgcataca aaattcccgc cagaccaagc aaaatcacca
    20101 gtaaccagat agccgcacca tcggaaagtt gtagcgactc acccacatga aacaggctgt
    20161 tgagcgccaa cgcgccggag tagagaacaa tcggcagaaa gcagacgccg gtggcaatga
    20221 ggaagcagaa gtcgatgata atacgcgtcg ttttatcata acgttcctcc agaaaatcgg
    20281 ggatggtggc aatgccgcgc ttcagatagc gcggtaaaaa gattagcgcg aggaagatca
    20341 gcgtcaccgc agaagtgact tcccagccca tcaccgacat gccgcttttg taggcctgcc
    20401 cggaaagtcc aaccagttgt tccgtggaaa ggttggttag cattaacgaa gcggcaataa
    20461 ccggcgcttt tagtgaacgt ccggcaagaa aatagccttg ttgcgatccg gtatctgttt
    20521 tgcgaacctt ccaccaggtg atgatcgcca ccagcagcgt aaaaccgaca aaactcaaga
    20581 tttgtaacga attcatctca tagcccttat ttatgtttat gtcccggacg catgaatata
    20641 gggagaggta aaatgcgtcg ggtcaggcag tcagattctt aaaatacaaa cgtcgtatcc
    20701 ctgaacggat tgttttctgt taagttcagg ttgtgagcat aattcgccgg agggatatta
    20761 aaatgaatgg aaaattgcaa agttcggatg taaaaaacga aactccgtac aatattccct
    20821 tattgattaa cgaaaatgtg atctccagcg gaatttctct gatatcgctc tggcatacct
    20881 acgccgacga gcattatcgg gtgatctggc cgcgggacaa gaagaaaccg cttatcgcca
    20941 actcatgggt tgcggtgtat accgtacaag gatgtgggaa aattctttta aagaatgacg
    21001 aacaaataac tctgcatggc aactgtatta tatttttaaa gccaatggat attcactctt
    21061 atcactgtga aggtttggtc tgggaacagt actggatgga atttaccccc accagtatga
    21121 tggatattcc cgtcggtcag caaagcgtta tttataatgg cgaagtttat aatcaggaac
    21181 tcaccgaagt tgctgagtta attacttcac cagaagcaat aaaaaataat ctggcagtcg
    21241 cttttctgac gaaaattatt tatcagtgga tttgtcttat gcacgcagtc ggtaaaaaag
    21301 atccacaacg gcggcaaatt gaaaaattaa ttgccacttt acatgccaat ctacaacaac
    21361 gctggagcgt agctgatatg gctgccacga tcccctgtag cgaggcctgg ttgcgtcgtc
    21421 tgtttttacg ctataccggc aagacgccga aagaatatta cctcgatgcg cgtctggatc
    21481 tggcgctatc gctattaaag caacaaggga actcggttgg cgaagtcgct gatacgctca
    21541 acttcttcga ctcctttcat ttcagcaaag cctttaagca taaatttggt tatgcgccgt
    21601 cagctgtgct gaagaatacg gacaggtaat aaccagaatg gcgagtgtgt aaatgactcg
    21661 atgatgttca gaaataccag gcggagtacc atttacggcg actccaccgg gaaaagagtt
    21721 aaccgatact ccagaagagc accgccagta attggggtgt gataatgcgc aggaacatca
    21781 ctaacggata gacagtggcg taagagagcg ccgccgcacc actggttgga tgaagattat
    21841 tagcaaacgc cagcgccgga ggatcggtca tggaacctgc cagcatcccg cacatggtca
    21901 ggtaattcat tttggctaac atccgcgcca gaatgccaac agtaatcagc ggaacggcgg
    21961 tgatcagggc accataacca atccagctta gcccttcgcc atccaccagt gtatggataa
    22021 aatcgccgcc ggatttcagg ccaaccaccg agagaaagag cacaatgcct aactcgcgta
    22081 gcgccaggtt agcgcttggt ggcataaacc agtacagctt gccgatactg ccgatacgcc
    22141 cgaggatcaa cgccataatc agcggaccgc ccgccagccc gagtttcaac gccgccggga
    22201 acccaggcac aaagacggga atagaaccta acaatacgcc aagcccgata ccaataaaca
    22261 ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca ttggcaacgg
    22321 catcaattgc ggacggacgc cccaccaggt tgaggatatc gccgaactgc aggctgatat
    22381 cgctacttgc aactaattca acaccagcac ggttcaggcg cgagataaca acgtcatagc
    22441 gttctttaaa gtgcaggtcg cgaatacgtt taccgagcac gttttcattg gtgaccacca
    22501 cacgcgcgac gcgcaaatca gtgcctttcg tagacagcga ggtatcgacc tcctgaccaa
    22561 tcaccagttg cgcattatgt aaatccgcag gctgccccac cagatgcagc aaatcgccca
    22621 gttgaataac ggtttctggc gaaggcactt ttagggtttc ttctcgtttc aatcgtgagc
    22681 aaataacttt atcgccattg agaatcggca catctttaat ggctaaatta tgcaaattag
    22741 gattctcaac tcggatgtta atcgtcctga ttagcgcacc gccgttggtt cgcgtggact
    22801 cgtgctgctg ggcctcagtc tcaacattga cgcggaaaat aacccgcagc atccacatag
    22861 tgaacaaaat gccgcaaatc ccaaatggat acgccatcgc atagctcatc cccatttgat
    22921 cgaccatcgc catcggtgtc cccaggtcgc gcaagatctg ctgccctgcc cccagcgctg
    22981 gcgtattggt gaccgcgccg gaaaaaatcc ccagcactac cggcagtgga atatcaaaca
    23041 gtttatgcag gatggcggta accagaccac cgatgatgac gatcagaaca gcaaacaggt
    23101 tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc cggccctacc tgaatcccga
    23161 tggtataaac gaacaggatc aggccaaact cctgaataac atgcagcata tcgctactta
    23221 atgtcatccc cgcctgagaa acaaaatggc cgacgatgat cccaccgaac agcacgccgc
    23281 caatgcccaa tccaacaccg cgaaacttga cgttgccgat aaacaacccg acgaccgcca
    23341 ccaaagccag aatactgacc gttaatgcta tatcactcat cgtccatttc tcttgaaata
    23401 acattttaat tgctacggat tatggctgaa tcggtgaagc agatttgcct tatagcgcac
    23461 attatcacgg gaaataacgt gccgaaatat cttaaacagg cgaaatttaa cgcattgctg
    23521 aagaaaaaag acaaaaaaaa agccccacca gaatggcggg gcaaagagaa tagctagtta
    23581 gctatttaac gcgggacgtt cgctgatagc gatacgctgc gctgcgatgg gttcaggctc
    23641 attacgaatt aaatcaatat gcagtaaacc gtttacgaag gttgcgccag agacttccat
    23701 attttcagcc agcgtaaagc tcagactaaa aggctgattc ataagccctt gatgcagcca
    23761 ttttttctct tcttttggct gctccggcgt gccttttacg ctcaggcgcg taccttccag
    23821 ttgaatctct aaatcttcct gacggaaacc tgccagcgca agggtaatgc ggtagtggtt
    23881 atcgtcgctt ttctcaatgt tgtacggcgg gaagctctgg ctttcaccgg cgttttgcag
    23941 tgcgttggcc agtttgtcaa aaccgatcca ttgacgcatc agtggggata aatcgaagtt
    24001 acgcatagtc atttctcctt ctaagaagcg agtaagtacc tgcaaatccg aagattcgca
    24061 tatgctccct gacggcgagc atggaaatgt caggccgcgc caggcggcct tagggaatta
    24121 gttgatttcg atacggcgcg gttttttcgc ttccggaatc acgcgttcga gatcgatata
    24181 cagcagacca ttcaccaggt tagcaccacg aacatgaatg ttctcagcta actggaattt
    24241 gcgttcaaag ttgcgttcag cgatgccctg gtacagatag gtgcgctctt tttgttcgtc
    24301 ggcatgagca cctttcacca ccagcagatt atcctgggcg gtaatttcca gttcgctctc
    24361 agcaaaacca gccacagcga tagcaatgcg gtaatggttt tcgtctacca gttcaacgtt
    24421 atacggaggg tagccgccat tactctggct ctggttgttt tctaagtggt taaacaaacg
    24481 gtcaaatcca atagcagaac ggtaaagcgg ggataaatca aagttacgca taatcaatag
    24541 ctcctgaaat cagcgagaat gtaagacctt ccacaatgga caggtcaggt agccagaaca
    24601 cccatcaggc gcgtcctcat cggctacgat gtaaaaatgg gtctggaaat gaacttttca
    24661 agccttatca gcggactttt ttttgcagtt tatggtctat tgcatagact gagggggcag
    24721 cacgaaacgt taaagtgcga tagccgccac agtgcgacgt aatgatgatg ttattttcag
    24781 caagggatcg ctataactca tcaggccaac acccacgatc aacagatgaa tttattatga
    24841 taagaaatgt gttgttagcg ttcatgatat gcagcggaat gacattactc ggaggatgct
    24901 ccagcgtgat gtcccatacc ggggggaaag aaggaacgta tcctggcacc cgcgccagcg
    24961 caaccatgat tggcgacgat gaaaccaact ggggcacaaa atcgctggcg atactcgaca
    25021 tgccatttac cgcagtactg gacacaatat tgctaccgtg ggatgtgttc cgcaaagaca
    25081 gctcagtgcg ctcgcgcgtt gaaaaaagcg aagccaacgc acaagccacc aatgcggtta
    25141 ttccaccggc cagaatgcct gataactgat ttagcgatca gtttcggtga tccacaactg
    25201 accgtcctcc atccacgcca accattgacc atccggcgag aaaaccacgg cgtccgcaga
    25261 aggtggattg gcgtggtttt cggttaaata ctcaacctcg ccgctttgcg catgggcaca
    25321 agcaatccga ttatccagca caaatcccag ccattcgcct gatggatgcc agttaaatgc
    25381 agactgaata tccgttttat tatgggttaa ctggcgtggt tcaccgccct gtggcgagat
    25441 aagccacagt tgcacaatgc cgttatcatc acgcattaaa aacgcgattt gcgtaccctg
    25501 cgggttacag cgcacccagt ggcgcgggac gttgactaac cccggataag cccgatggtg
    25561 ggtaaaggtt aaacgtcgct gcacgacgcc acgcggtggc gcgggcaggg ttgtttccgt
    25621 tccacttaac ggcgcatcac cagctgcttt ccagccagct tcatctttcg gcaactcgac
    25681 aataaacagc tccggcactt tctcgccctt tggcgaaagt gtgtcgccaa taaacgccag
    25741 tgcgtgattt cctacccatc cttcttcata agcacgattg atttcattac tgccaggctg
    25801 cggcgtgggc gttgttttac tcaccagtac gcaccagtgg ctaccgctgt attcacgcgg
    25861 atgctgcttt tgtacgttga ccgggccaaa cggtgcagca acaccgacat ttcgcaaatc
    25921 cagcgccgga tcgagttcat gcattacatg gtcgttgtag gtaaagctca ccctttcacc
    25981 gtttgggcta aagacatgta catgactgcc gccgcgcagc gctcctggag tatatgacgc
    26041 ggtaatatcc attgcatcga gattgctcac cttgccgctt tcaacaatca ctccgcgccg
    26101 atgatgaaag tcgtaatacc atgtttcatc aggattttcc gggccgtgga tgaagacata
    26161 cttctctgac ttcggatgaa cggtcaccac gccgacgtat gcgccctgtg acgcgcgata
    26221 gataacctcg acctcgccgg tatgaatatt cacacgctca atggtctcgc cggtaaacga
    26281 cgccccagaa ggccgcacgt caaacaccag ccactggctg tcgggcgtcc aggtattggt
    26341 attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc ggtcctgata
    26401 caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat agtcttttgc
    26461 catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcaatta ttggcctcgg
    26521 cccggcaggg tcggcgttgg cgcgaaagtt agcaggcaaa atgcaggtga tcgcgctgga
    26581 taaaaagcac cagcatggta ctgaaggttt cagcaaacct tgtggcggtc tgctggcacc
    26641 ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg tcatcgccaa
    26701 cccgcagatt ttcagcgtca aaaccgtcga cgtcgccgca tcgctcacgc gtaactacca
    26761 gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat cgctgatccc
    26821 cgccagcgtt gaggtttacc acgacagcct gtgtcgaaaa atctggcgtg aggatgataa
    26881 atggcatgtc atttttcgcg cagacggctg ggagcagcat attaccgccc gctatctggt
    26941 cggtgccgat ggtgccaact cgatggtgcg gcggcatctc tacccggatc atcagattcg
    27001 taaatatgtc gccatccagc agtggttcgc agagaaacat ccggtgccgt tctactcctg
    27061 catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag acggttattt
    27121 tatctttggc ggtgcctatc cgatgaaaga cggtcagacg cgtttcacga cgctaaaaga
    27181 gaaaatgagt gcctttcagt tccagtttgg taaggcggtg aaaagcgaaa aatgcacggt
    27241 gctgtttccc tcacgctggc aggattttgt ctgcggcaag gacaacgcct ttctgattgg
    27301 cgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg cgctggatag
    27361 cgcagagatt ctgcgttcgg tattactgaa gcagccagag aagctcaata ccgcttacag
    27421 gcgcgccact cgcaaactgc gtttaaaact cttcggcaag atagtaaaaa gccgatgcct
    27481 gaccgcaccg gctttaagaa agtggattat gcgcagtgga gtggcgcata ttccacagtt
    27541 gaaagattat ccaacgcgct tcacatcgcc caccagcagg atgtaagaga gcgcgccaat
    27601 caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct gcgccaggta
    27661 tcccaccacc agcggaacgg tgatgccgcc cagaccaccg gcaaagttaa acacgccgcc
    27721 ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg ttatcgaagc
    27781 aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg gatcgttggt
    27841 gtagttcgca cccataatgc aggtggagat cagcaagccg cagataatcg gcgttttacg
    27901 cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc cagagagcag
    27961 gacgccaata aacgccgcga ggaacggcac cgtggtcata aagcctgctt tcagcgctgt
    28021 gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc acagtgtaga
    28081 agccaccgca aattgcccaa gatagacacc gatcagttta cgatggaaca ccagtttcca
    28141 gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcgc catccaccag
    28201 accgccgcca tcacgaatgt aatccagttc agctttgctg atgcctttgg tcagacgcgg
    28261 cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac cggtgacaat
    28321 gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag tcagaaacgc
    28381 cagaccgaca aactgaccag acgtataaaa accaacggca gaagcgcgtt catgttccgg
    28441 gaaccaactg gttaccatcc ggttattggt cgggaacgcg ggcgcttcga aaataccggt
    28501 tatcgcgcgc agaccaatta atgacattaa gcccgtggca aagccctgga ataacgtcgc
    28561 cactgaccag ccaaatatcg caataaaata agtcacgcgt gaacctacgc gatctaaaaa
    28621 ccaaccaccg gggatctgac atagcgtata aagccaggcg aaggccgaaa atacatagcc
    28681 catttgcgct ttagtaatac caaactcttc ctgaatatgg gcagaagcca cggccaggtt
    28741 agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg tcagataacg
    28801 ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc tccagatttt
    28861 gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat ttttgtattg
    28921 ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc tacgctgtta
    28981 tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa ctcaataact
    29041 ttagcttcgt cgatttccac gcctaagccc ggtttcgtta atggtttaaa gaaaccgccg
    29101 accatgctga agtcctcttt gtttttcaca aagtcgagta actccgcgcc tttgttgtaa
    29161 tgaatgccca tactttgttc ctgaaggacg gcgttatagg aaacaaagtc gatatgcagg
    29221 caagccgcca gtgcaatcgg tccgagcgga cagtgcggcg caagggtcac gtcataggct
    29281 tctgccattc cggcaatttt gtagcattcg gtaataccgc ctgcgtggga gagatccggt
    29341 tgcagaatcg aaataccgcc tgcctccagc acacgtttaa aatcgaagcg tgagaacatg
    29401 cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca gtttcgggta gtattcggcc
    29461 tgttccgcca gcaccggctc ttcaataaac agcggacgat acggttccag ctctttaatc
    29521 agcactttcg ccatcggcgc gctgacgcga ccatggaaat caagaccaaa ctcaatctga
    29581 ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac cgcgcgggag
    29641 ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa gccgatttcg
    29701 cgtagcgttt taatgccgtc gataacatcc gccggacgat cgccgccgac ccaactgtag
    29761 gctttaattt tgtcgcgaac caggccgccc atcagttgcc agaccggcgc attcagcact
    29821 ttacctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat caggattgga
    29881 ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat gcgtgacggg
    29941 tcctgaccaa tcaaatagtc actcagctcg tggactgccg cttccaccgt gcgagcgcgg
    30001 ccttcaatca cgggctcgcc ccaaccgacc acaccttcat cggtttcaat tttcaggaac
    30061 atccagcggg gaggtaaccg atacgtggta attttggtga ttttcattgc actgcctctc
    30121 gatacgcctt aacaaatgct gctgcctgct gcgcagtccg ttctacggac tgcccggcgc
    30181 gatagagatc gctgcctaag cccgcccctg cacaacctgc gtctatccac tgcgccaggt
    30241 tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat accgctttta
    30301 atgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc gcctgcgcgc
    30361 ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcaca gccggggcag acggtcatgc
    30421 cgtagcccac cgcacggcgg atcacttcac tatggatatt gggcgtaacg atgagctgac
    30481 agcccattct ggcgagcgca tcgacctgtt caggtttcag taccgtacct gcgccaatca
    30541 acgccttatc gccgtacgca tcaacgatgg cgggaatgct ctgctcccat tgtggagaat
    30601 tcagcgggat ttcaaccgca tcgaacccgg cgtcaatcac cgcgccaaca tgtgccagcg
    30661 cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt tgccactgca
    30721 tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ctgccaccgc cgtcacgtcg
    30781 caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc ggcgacaagg
    30841 gtgatggcgt gttgatgggc cacatagtca cgcatactgg caacctctgc gccaatcaac
    30901 aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac atgcgaggcg
    30961 cgaacctcaa aaagctgtgg caatacggcg ggcgcattaa ggccacgctc aagaccagcc
    31021 gcgaaggcat cggcagcgtt ttcctgcggc ggcaaacctg cgccaatcaa tgagtgattt
    31081 aacagtaaat gatgtaattc accgctcatc acggtgcgaa aatcgttaat ttgctggcta
    31141 tcggcctgca cccatttgca atgcgtaccg ggcatgacat aaagagagga aggagcgaga
    31201 gtgcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt atcgtcatga
    31261 gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa ttgttcgcca
    31321 atagacgaaa aacgggctgg aacggataaa tacggcgcaa ctttccagcc gacattgctg
    31381 ccgaccattc ctgccattac cactggcgta ttctcttcac gccagtcggt cgtcacttct
    31441 gctaacaccg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc tgattgcctg
    31501 cagtccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga tccccagtca
    31561 attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag ctggcgatca
    31621 tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccttatcgca tcgaacagcg
    31681 ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag gttcgttcaa
    31741 aaaccgcccg ctgcagcgaa ctgatcgcaa tgctcagttg ctgtaacacc gggttatgta
    31801 ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct tcgcggtcct
    31861 gattgttagc aatcatctcg ttcagcgccg attcaatctg cgccagatcg ctggaggtcg
    31921 cgcgctctgc tgcccaacgg gcaatcgccg gctctaccag gtttcgcact tcgctcattg
    31981 cactgataag ccgtgggtca tagtcgtttt ccagcaccca ttgcagtacg tcagtgtcga
    32041 ggtaattcca ctggttacgc ggagccacaa acgccccgcg ataacgtttc atttcaatca
    32101 gccgcttcgc catcagcgaa cgaaatacct cacggatgat gttgcgcgag gttgcaaact
    32161 cctcacagag ttctgcctca gccggaagcg gcgagcctgg cacgtatttg ccgtgaacga
    32221 tctgtttacc cagcgtaata acgatgcgat cggttttatt gagagtcatg gagagtcctt
    32281 gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc aattttgtag
    32341 taccagcgtg atgacgttcg cgtttgctgt gcgcctaatg tagtacaaag ttatattgtt
    32401 gtactacaat ttagatcaca aaaagaacaa tccataaaaa aatgttctac gtcgggcaaa
    32461 aatctgaaaa gggagatcag gcgctaaact ggggggaaag aagagtatgc tttcaacggc
    32521 ttagtactct tgtttaaagg attaatcatg aagttgaatc ttaagggatt tttcaaggct
    32581 gccagtttgt gttctttggc atttgcgttg accggatgta tcacctgggg attagtttcc
    32641 aatactgcaa gccatccaca tgccgagcaa tggcggtcag acacgattaa gggtttatcg
    32701 ctggcagaag atagtaacgg tacgaaaggc tatgtttttg ttggtgaatc gttggactat
    32761 ctgttgacca ccggcggcga tgaggtggtt aacatgctga acgatccggc aatccatggt
    32821 gaaagaatca ccgtttctga taacgcgaaa tttattttaa gcagcagtaa taagaatttc
    32881 tctggtgcta ttacgctcta ttacgactgg aacaacgaag aagataaggc actggcaaca
    32941 caatatggtt ttatttgtga tacccggcgt tgtacatgga tgctggacgg tttgaaaggc
    33001 tcaatccacc agaaaaataa aaaagcggat tactcaaacg tgatggtttt ccatcaaccc
    33061 tttacggtgg gattttatga gtacaaagca acagatggcg tcccgcgcgg cctcgttaat
    33121 gcgctgctgc cagtcactct tacgctggat atagtaacgt caccactcca gttcctgatt
    33181 ttatgcacga ccagaaattg ttaatcgctg cgggcaaacg cattcatgcc gtttgcccgt
    33241 tcactgatta attcagcaca tacttctcaa tagcaaatgc cacgccatct tcaaggttgg
    33301 atttggtgac aaagttcgcc acttctttca ccgaaggaat agcgttatcc atcgccacac
    33361 cgacgcctgc atattcaatc attgcgatgt cgttttcctg atcgccaatc gccatgattt
    33421 cttccggttt aatacctaac acgtcggcca gtgatttcac acccgtacct ttgttaacgc
    33481 gtttatcgag gatttcgagg aagtacggcg cacttttcag cacggtgtat ttttctttca
    33541 cttcctgcgg aatacgcgca atggcctggt cgaggatggc gggttcatca atcatcatca
    33601 ctttcaggaa ctgggtattg gggtccattt tctccgcttc gcagaacacc agcggaatgg
    33661 tggcaacgaa agattcatgc accgtgtagt agctgatatc acgattggcg gtgtacagcg
    33721 tggtgcggtc cagcgcgtgg aaatgagaac cgacttcgcg agagagtttt tccaggaaac
    33781 ggtagtcgtc atagctgaga gcggtttgcg ccacggtgct accatcagcg gccttctgta
    33841 ccagcgcgcc gttataagta atgcagtagt cgcccggctg ttccatatgc agctctttca
    33901 ggtaattgtg aacacctgca taagggcgac ccgtcgttag cacgacattc acgccacggg
    33961 cgcgagctgc ggcaatcgca tttttaacgg cgggtgaaat ggtgtgatcg ggcagcagaa
    34021 gggtgccatc catatcgata gcaatgagtt tgatagccat gagttcccca ggtagattgg
    34081 ttcctgaccc atgctaacgc gattccgctc aaaaatcagt acaacacccg agggaaaagg
    34141 gggatgcaac gcgcattgcg tgctccccat ttgcttagcg gaaaagtttc cctttcagca
    34201 gttccatgcc tgcggaaagc agatcgttat tggcttgtgg tgacacttca ccttgcggtg
    34261 agagcgcatc aataatcttc ggcaattgtt ctgccagtaa actggaagct gtactggtat
    34321 ccacgccaag tttttgcccg agatcgaaca ccgcatttgt gccgagcgcc gattccactt
    34381 gctcgccgct aactgattga ttgctctgtt gattactcag ccaggttgag agaatggccc
    34441 ctaagccgcc actttgcagt ttttccaaca gcacctgaat gccgccctgt tcctcaaccc
    34501 agctcaaaat ggcctgatat ttccccgcat cgcctttcag aaaggcaccg acaacgtcat
    34561 caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa agtatagatc
    34621 tgcgggatga taattgcgga ttgcagaaat aaaaagggtg gagatgatct ccgccctttt
    34681 cttatagctt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa aacatgcaaa
    34741 ttcaatatat tgcaggagtc tcgtaggcct gataagcgta gcgcatcagg cacgctcgca
    34801 ttgttaacgc cattaaatat cgatattcgc cgctttcaga gcgttctctt caataaacgc
    34861 acggcgcggt tcaacggcgt cgcccataag cgtagtgaac aactggtcgg cagcaatcgc
    34921 atctttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg tttcccacag
    34981 ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc cgcgacggga
    35041 ctctttcacc agccagtcca gcgcctgctc gaagctggct accggttgac gacgttcgcc
    35101 acgttcgata aatgcatctt cttccagcaa gccacgcagt ttctcaccca gcgtgcagat
    35161 acgacgatat tcgccgccag tgataaattc gtgatccagc ggataatcag tatccacacc
    35221 gtgggtacgc acgcgaacaa tcggctcgaa caagttttgc tcagcattgg tgtggacatc
    35281 aaacttccac tggctgccgt gctgttcttt atcgttcagt tcgctgacca gcgcgttcac
    35341 ccagcgggta acggtctgct cgtcagaaag gtcggtttcc gtcagcgtcg gctgatagat
    35401 aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca ttttctgcgt
    35461 cgcgttgtac tcagacacca gtttctctaa cgcttcgcca gccagcgccg gtgcactggc
    35521 gttggtgtgc agcgttgcgc catccagcgc gatagagatc tggtactgat ccatcgcttc
    35581 gtcgtcttta atgtactgtt cctgcttacc tttcttcact ttgtacagcg gcggctgagc
    35641 gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga aggtcaacag
    35701 cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga tgctgtgata
    35761 acgcagtttg tccgggttgt actcgtcacg accgatacca caaccaagcg cggtgataag
    35821 cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga cgttgaggat
    35881 tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct tcgcagagcc
    35941 gcccgcggag tccccttcca caaggtacag ttcggaaagc gccggatcgc gttcctggca
    36001 gtctgccagt ttgcccggca ggccagctaa atccagcgca cctttacggc gggtcatttc
    36061 acgcgcgcga cgcgcagctt cacgggcacg ggcagcatcg ataattttgc cgaccacgat
    36121 tttcgcgtcg gttgggtttt ccagcaggta ttccgccagc agttcgttca tctgctgttc
    36181 aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgtgagg agaatttcgg
    36241 gtccggcact ttcacggaaa cgaccgcaat caggccttca cgcgcatcgt caccggtggc
    36301 gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcgttca gggtgcgggt
    36361 catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa tgttgttggt
    36421 aaagcagtag atgttttcct ggaagccatc gttccactgc aacgccactt cgacgccaat
    36481 accgtctttt tcggtggaga agtagaagat attcgggtgg atcggcgttt tgttcttgtt
    36541 cagatattca acgaatgcct tgatgccgcc ttcatagtgg aagtggtctt ctttgccatc
    36601 gcgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact cacgcagacg
    36661 tttcgccaga atgtcatatt cgaactcggt cacattggtg aaggtttcaa ggcttggcca
    36721 gaaacgcacc atagtgccgg ttttttcagt ctcgccggta accgccagcg gggcctgcgg
    36781 tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctggataa ccagctccag
    36841 tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc cggacacttt
    36901 ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca cttccgccgc
    36961 cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat cctgtacaga
    37021 gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg cgagcgcttc
    37081 gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc cgtcatccgt
    37141 gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca ggactttgat
    37201 actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac ttgggttaat
    37261 ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac atgtctataa
    37321 cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt aagcggctgg
    37381 caagcagccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc aggtagagac
    37441 accgccgccc gctttcacgg gtgaggaact ctccttgcgc cagacgtaag gcgcacatca
    37501 acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg gcgcgaatgc
    37561 gtaagtccgc tttatgcggg ccgtgcgcgg tgtaggttag ctggcgatcg cgttcaaaat
    37621 tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg aaagagaaag
    37681 tcagagaaaa ctcagggaga aattgcttac aggtatcagc catatcggct gcgataccgg
    37741 cgctatactc cgcgcgccag gtgctgattt gctccgcctg cgggatcagt tctttatccc
    37801 acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc tgcttgagca
    37861 atcgcttgag attgctccag gcggtgaaaa atccgggttc gttgtgaaag catccccagt
    37921 cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct tctggcgtta
    37981 tcagctgcat tggcatcagg tgcgccagct ccgcgacttt atgcccgtct gtaccgtcaa
    38041 tacggacttt gctgtcgcct tgtttgtctt tggttaaacc aatcgccgtc tcgcgctctt
    38101 cgccctgtaa tcgcccgtgg agaacaaacg cctcctgctc gtggcgaatg acgcgaccaa
    38161 tctgcaaact gcgaaacgcc cgaccgtggc cgagcgtata gatggcttcc agcacgctgg
    38221 ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataag gcgagatccg
    38281 cggtttcaat gttgcggaaa tcgcggatca acaagcgggt gagggacatt acagtctcat
    38341 tggcatgaca acataagccg cgctctggct ggccgcatct tcaatctgca cgctggaaac
    38401 cgaatcggtc agcatcatgc ggacgttttc gcatttcagt gcgttcagaa catccagcac
    38461 atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt cgaggatctc
    38521 ttccgcttct tcctgttctg ggttgttggc ggtgattttc agctggtttt cgctgacata
    38581 cagacgcacg ccgcggaatt tctcgttaga gagaattgcc gcacgagcaa acgcctgctt
    38641 gagcagatca cagccagctt ccagatgttt gtccgggttc ttcggcagaa cgcgacggta
    38701 atccgggaag cgaccatcca ccagtttcga ggtgaagata aagtcgccaa cgtgggcgcg
    38761 aatgttgttg ctgccaatct gcacgcgcag cggattgtcg ccgccgtcga gcatacgcat
    38821 cagttcaatc acgcctttac gcggcacgat caccgaatgg cttggcaaag attgaccaat
    38881 tggcattgaa cagactgcca gacggtggcc gtcggtcgcc acggtgcgca gttcttcacc
    38941 ttcggtttca aacagcatac cgtttaagta atagcgaacg tcctgatgcg ccatagaaaa
    39001 ctgggtcgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt cgacttcgct
    39061 ctgccagtca tcgaggttcg ggaaatctgc cgctggcagg gtagacagcg aaaaacggct
    39121 acgcccggag cgcaccagca tccgctcacc ttccagctgc acggcaattt ccgcgccttc
    39181 aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg cccctggttc
    39241 gtgcggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag taccggtcag
    39301 cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta gcgtaggacg
    39361 accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac gttctacggt
    39421 aaatttcata ggtttacgat gacaatgttc tgattaaatt agaaaaatct tctttgatat
    39481 cgtggctctc ttcacgcaac tgctcgatct tccggcaggc atgaagcacc gtcgtgtggt
    39541 cacgaccacc aaaggcatcg ccaatctccg gcagactgtg gttagtcagc tctttcgcca
    39601 gcgccatcgc catctggcgc ggacgggcca ccgagcggga tcgacgcttg gaaaggagat
    39661 ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg atggtgacca
    39721 gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg atggtgatcg
    39781 ctcgtccggt aaagttggcg ttggcaatga cgcggttcaa cgcgccttcc aactcacgta
    39841 cgttagatcg tagacgcttg gcgataaaga acgccacttc gcccggcaaa cgaatgtcgt
    39901 tttcgtcggc ctttttcatc aggatcgcca cgcgggtttc cagctctggc ggttcgatcg
    39961 ccacagtcag tccccagccg aagcgggatt tcaaacgatc ctcaacgccg ttgatctctt
    40021 tcggatagcg atcggaggtg agaatgatct gttgattacc ttccagcagg gcgttgaagg
    40081 tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata tcgtcgatca
    40141 gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg ttttgcagag
    40201 ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg gcattcggct
    40261 tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc aaacccgtgc
    40321 cgccataaag gaacaacggg ttataagcac cgccagggtt atctgccacc tggcgagccg
    40381 ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg tgtttgacgt
    40441 ttacgttaga acgataggtc ggttctgccg gagccgggac gttatcccaa cccgagcgcg
    40501 tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc gcgacgttgc
    40561 tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca aaacgcagct
    40621 gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta aggtacttgt
    40681 cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg ttatcgctca
    40741 gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac tcatcctgca
    40801 atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact cgaacaaaag
    40861 tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa agaccctgca
    40921 tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc ctgcaaaacg
    40981 atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct cacagatttt
    41041 cccgatttat ccacaggatg atccagaact cgctaagtgt aaacgatcct gccgcgggcc
    41101 gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa ttggcttaat
    41161 cgatctaata aagatccagg acgatccttg cgctttaccc gtcagcccgt ataatcctcc
    41221 acccggcgcg ccatgctggt tttcactggt gtgaggccgt acgttttccc cgcgaaaagg
    41281 tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggagtgtaca attattacaa
    41341 tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc ggcggatatc
    41401 caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac gcacttttca
    41461 accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg ctactaaaaa
    41521 tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga ccgtttctaa
    41581 gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta cgcttgttaa
    41641 ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg ccgcaaatta
    41701 ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca gtcgccaaga
    41761 aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg tctgacgcgt gaaagcttcc
    41821 gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa aaaggggttg
    41881 ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc cgccactgtc
    41941 gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga ttagtccgct
    42001 actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg aggcattgcg
    42061 caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa aatgccaccc
    42121 tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca gagaacacta
    42181 acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc tttcatgatc
    42241 tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac cacgcagaca
    42301 acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg ccaggggaaa
    42361 ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg tggtgatgtt
    42421 gagcaagccc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc gttccagctg
    42481 ctggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg tcgtgatggc
    42541 ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga cgcttatgtg
    42601 ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc ggcaggcaac
    42661 acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt caactacaac
    42721 gtgcagaacg ctggcgagaa accgctggaa atctccacct ttggtcagtt gaagcaatcc
    42781 atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca caccttccgt
    42841 ggtgcggcgt actccacgcc tgacgagaag tacgagaaat acaagttcga taccattgcc
    42901 gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcaatgct gcaacagtat
    42961 ttcgcgacgg cgtggattcc gcataacgac ggtaccaaca acttctatac cgctaatctg
    43021 ggtaacggca tcgccgctat cggctataaa tctcagccgg tactggttca gcctggtcag
    43081 actggcgcga tgaacagcac cctgtgggtt ggcccggaaa ttcaggacaa aatggcagct
    43141 gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat ctctcagcca
    43201 ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt ctccattatc
    43261 atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca gtacacctcc
    43321 atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg tctgggcgat
    43381 gacaaacagc gcatcagcca ggaaatgatg gcgctgtaca aagctgagaa ggttaacccg
    43441 ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt gtactacatg
    43501 ctgatgggtt ccgttgaact acgtcaggca ccgtttgcac tgtggatcca cgacctgtct
    43561 gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt cttcattcag
    43621 aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac ctttatgccg
    43681 gtcatcttca ctgtgttctt cctgtggttc ccgtcaggtc tggtgctgta ctatatcgtc
    43741 agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga aaaacgtggc
    43801 ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa ataagggcgg
    43861 tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcacatc cgccacacaa
    43921 agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac gcctccggga
    43981 cgtggcggcg ttggcatcct gcgcatctcc ggcctcaaag cccgtgaagt tgccgaaacc
    44041 gtgctgggta aactgcctaa gccacgctac gccgattatc ttccgtttaa agacgccgat
    44101 ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc gttcaccggc
    44161 gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct gctgttaaaa
    44221 cgcattctga ccattcccgg cctgcggatt gcccgccctg gtgagttttc cgaacgcgca
    44281 tttctgaacg ataaactcga cttagcccag gccgaggcga ttgcggatct tatcgacgcc
    44341 agctcggaac aggcggcccg ttcggcgcta aactcgctgc aaggcgcatt ctccgcacgg
    44401 gtcaaccatc tggtggaagc cctcacccac ttgcgcattt acgtcgaagc ggcaattgat
    44461 ttcccggatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca gctcaatgac
    44521 gtaattgccg atctcgacgc ggtatgcgcc gaagctcgtc agggtagttt gttgcgcgaa
    44581 gggatgaaag tggtgattgc cggacgtcct aacgccggta aatcgagcct gctgaacgcg
    44641 ctggcgggcc gtgaagcagc aatcgtaacc gatatcgccg gaaccacgcg tgacgtgctg
    44701 cgtgagcata tccacattga cggaatgccg ttgcatatca tcgataccgc cgggcttcgt
    44761 gaagccagtg acgaagttga acgtattggt atcgagcgcg catggcagga aattgaacag
    44821 gcagaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga tccggcagag
    44881 atctggccgg aatttatcgc ccgtctgcca gcgaaactgc cgatcaccgt ggtgcgcaat
    44941 aaagccgata tcaccggcga aacgttgggg atgagcgaag tgaacggtca tgcgttaatt
    45001 cgtctttcgg cgcggactgg cgaaggcgtg gatgtactgc gtaaccatct caaacagagc
    45061 atgggctttg acaccaatat ggaaggcggc ttcctggcgc gtcgtcgtca cctacaggcg
    45121 ctggaacagg cggcagagca tttgcagcaa ggtaaagcgc aactgttagg tgcctgggcg
    45181 ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga gatcaccggg
    45241 gaatttactt cagacgacct actggggcgg attttctcca gtttctgtat cggtaagtaa
    45301 ccgcgcttgc gaagccgcat tctgactgtc agatgcggct tcgcttcatt gttagcactc
    45361 ctgttattcc tcaacccttt tttaaacatt aaaattctta cgtaatttat aatctttaaa
    45421 aaagatattt aatattgcgt cccgaacgat tgtgattcga ttcacattta aacaatttca
    45481 gaatagacaa attccttgag tattatactg tagcctcgtg tctttcgagg ataagtgcat
    45541 tatgaatatc ttacatatat gtgtgacctc aaaatggttt aatattgaca acaagattgt
    45601 cgatcaccgc ccttgatttg cccttctgta gccatcacca gagccaaacc gattagattc
    45661 aatgtgatct atttgtttgc tatatcttaa ttttgccttt tacaaaggcc atctctcgtt
    45721 tatttacttg ttttagtaaa tgatggtgct tgcatatata tctggcgaat taatagatat
    45781 tgcaaatgta atattcacag ggatcactgt aattaaaata aatgaaggat tatgtaatgg
    45841 aaaactttaa acatctccct gaaccgttcc gcattcgtgt tattgagcca gtaaaacgta
    45901 ccactcgcgc ttatcgtgaa gaggcaatta ttaaatccgg catgaacccg ttcctgctgg
    45961 atagcgaaga tgtgtttatc gatttactga ccgacagcgg caccggggcg gtgacgcaga
    46021 gtatgcaggc cgcgatgatg cgcggcgacg aagcttacag cggcagccgc agctactatg
    46081 cgttagccga gtcagtgaaa aatatctttg gttatcaata cactattccg actcaccagg
    46141 gccgtggtgc agaacaaatc tatattccgg tactgattaa aaaacgcgag caggaaaaag
    46201 gcctggatcg cagcaaaatg gtggcgttct ctaactattt ctttgatacc acgcagggcc
    46261 atagccagat taacggctgt accgtgcgta acgtctatat caaagaagcc ttcgatactg
    46321 gcgtgcgtta cgactttaaa ggcaactttg accttgaggg attagaacgc ggtattgaag
    46381 aagttggccc gaataacgtg ccgtatattg ttgcaaccat caccagtaac tccgcaggtg
    46441 gtcagccggt ttcactggca aacttaaaag cgatgtacag catcgcgaag aaatacgata
    46501 ttccggtggt catggactcc gcacgctttg ccgaaaacgc ctatttcatc aagcagcgtg
    46561 aagcagaata caaagactgg accatcgagc agatcacccg cgaaacctac aaatatgccg
    46621 atatgctggc gatgtccgcc aagaaagatg cgatggtgcc gatgggcggc ctgctgtgca
    46681 tgaaagacga cagcttcttt gatgtgtaca ccgaatgcag aaccctttgc gtggtgcagg
    46741 aaggcttccc gacatatggc ggcctggaag gcggcgctat ggagcgtctg gcggtaggtc
    46801 tgtatgacgg catgaatctg gactggctgg cttatcgtat cgcgcaggtg cagtatctgg
    46861 tcgatggtct ggaagagatt ggcgttgtct gtcagcaggc gggcggtcac gcggcattcg
    46921 ttgatgcagg taaactgttg ccgcatatcc cggcagatca gttcccggca caggcgctgg
    46981 cctgcgagct gtataaagtc gccggtatcc gtgcggtaga aattggctct ttcctgttag
    47041 gccgcgatcc gaaaaccggt aaacaactgc catgcccggc tgaactgctg cgtttaacca
    47101 ttccgcgcgc aacatatact caaacacata tggacttcat tattgaagcc tttaaacatg
    47161 tgaaagagaa cgcggcgaat attaaagggt taacctttac ctacgaaccg aaagtattgc
    47221 gtcacttcac cgcaaaactg aaagaagttt aattaaaaca ctcagagtgg ctataaggat
    47281 gttagccact ctattaccct acatcctcaa taacaaaaat agccttcctc taaaggtggc
    47341 atcatgactg atcaatcaga aaaaaaacac tctgcatttt ggggtgttat ggttatagcc
    47401 ggtacagtaa ttggcggtgg gatgtttgct ttacctgttg accttgccgg agcctggttt
    47461 ttttggggcg cttttattct tattatcgcc tagttttcaa tgttacattc agggctatta
    47521 ttattagaag caaacttaaa ttatcccgtc ggatccagtt ttaacaccat caccaaagat
    47581 ttaatcggta acacctggaa cattatcagc ggtattaccg ttgccttcgt tctctatatc
    47641 ctcacttatg cctatatctc tgctaatggt gcgattatta gtgaaacgat atcaatgaat
    47701 ttgggctatc acgctaatcc acgtattgtc gggatctgca ctgccatttt cgttgccagc
    47761 gtattgtgga taagttcgtt agccgccagt cgtattacct cattgttcct tgggctgaag
    47821 attatctcct ttgtaattgt ttttggttct ttcttcttcc aggtcgatta ctccattctg
    47881 cgcgattcca ccagcaccac tgcggggacg tcttacttcc cgtatatctt tatggctttg
    47941 ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc ccagcctgat tatttgctat
    48001 ggcaaaaaga aagacaaact aatcaaaagc gtggttttcg gttcgctgct ggcgctggtg
    48061 atttatctct tctggctcta ttgcacgatg gggaatatcc cgcgagaaag ctttaaggcg
    48121 ataatctcct caggcggcaa cgttgattcg ctggtgaaat cgttcctcgg caccaaacag
    48181 cacggcatta tcgagttttg cctgctggtg ttctccaact tagctgttgc cagctcgttt
    48241 tttggtgtca cgctgggatt gttcgattat ctggcggatc tgtttaagat tgataactcc
    48301 cacgccgggc gtttcaaaac cgtgctgtta accttcctgc cacccgcgtt gttgtatctg
    48361 atcttcccga acggctttat ttacgggatc ggcggtgccg gactgtgcgc caccatctgg
    48421 gcggtaataa ttcctgctgt actggcaatc aaagcccgca agaagtttcc caatcagatg
    48481 ttcacggtct ggggcggcaa tcttattccg gcgattgtca ttctctttgg cataaccgta
    48541 attttatgct ggttcggtaa cgtctttaac gtgttaccta aatttggcta aatccttcag
    48601 gaagccagcc attcgctggc ttcttgcctg tcaggaaatc acttatgtcc aaatggcaac
    48661 tcgcctgatt ctccttcacc acgtatgctt tgcgtcacct tactatcagg acgctttagc
    48721 ccatgtcccg ctttttgatt tgtagttttg ccctggtttt actttatccc gcagggattg
    48781 atatgtacct cgttggttta ccgcgcatcg ccgccgatct caatgccagc gaagcgcagt
    48841 tgcatattgc gttctccgtt tatctggcgg ggatggcggc tgcgatgtta tttgccggta
    48901 aagtagccga tcgttcaggg agaaagcctg tcgccatacc cggcgcggcg ctatttatta
    48961 tcgcctcggt gttctgttca ctggctgaaa ccagcgcgtt atttcttgca gggcgatttc
    49021 tacagggact gggcgcgggc tgttgttacg tagtggcgtt cgccattttg cgcgacacgc
    49081 tggatgatcg acgtcgggct aaagtgctgt cgttactcaa cggcattacc tgcatcattc
    49141 cggtgttagc gccggtgctc ggacacctga ttatgcttaa attcccttgg cagagtctgt
    49201 tctggacgat ggcaacgatg ggcatcgcgg tactgatgtt gtctttgttt attttaaaag
    49261 aaacgcgccc tgcggccccc acaacttcgg acaaaccacg agaaaatagc gagtcactgc
    49321 ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct cagcgtttcg gtgatcctca
    49381 ccttcgtcaa cacgtcgccg gtattgctga tggaaatcat gggttttgag cgcggagaat
    49441 acgccaccat tatggcgttg accgctggcg tcagcatgac cgtttcattc tccacgccgt
    49501 ttgcgctggg aatttttaag ccacgtacgt tgatgatcac ctcacaggtg ttattcctgg
    49561 cggcggggat cacccttgct gtttcacctt cccatgcggt ttcgctgttt ggtatcacgc
    49621 tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat gagtcaggcg ttagggccgt
    49681 tttcattacg cgcgggcgta gccagctcga ccttaggtat tgcgcaagtt tgcggttcgt
    49741 cactgtggat ttggctggcg gcggtggttg gtatcggcgc atggaatatg ctgatcggga
    49801 ttctgattgc ctgtagcata gtgagcctgt tgctgattat gttcgtcgcg cctggacgcc
    49861 cggttgccgc tcatgaagaa atccatcacc acgcttgatc tcaatttgct gctctgtctg
    49921 caactgctga tgcaggagcg cagcgtgacc aaagcggcga agcggatgag cgtgacgcct
    49981 tcggcggtga gtaagtcgct ggcaaaactt agaatgtggt ttgatgaccc gctctttgtg
    50041 aactcaccgc tgggcctgtc tcccacaccg ctaatggtca gcatggagca aaatctggcg
    50101 gaatggatgc aaatgagcaa tcagttgctg gacaaaccac tccacgaaac gccgcgcggc
    50161 ctgaagtttg aactggcagc agaatcaccg ctaatgatga tcatgcttaa tgcgctgtcg
    50221 aaacggatct accaacgtta cccgcaggcg accatcaaat tacgtaactg ggattacgat
    50281 tccttagatg ccattactcg tggtgaagtg gatattggtt tttccggtcg cgaaagtcat
    50341 ccccgttcgc gggagctgtt aagctcgcta ccgttagcca ttgattatga agtgctgttt
    50401 agtgatgtgc cctgcgtctg gttacgcaaa gatcatccgg cactgcatga agcatggaat
    50461 ctggacacct ttttacgtta tccgcatatc agcatttgct gggaacagag cgatacctgg
    50521 gcgctggaca atgtgttaca ggagctgggg cgcgaacgca cgattgctat gagcctgccg
    50581 gaattcgagc agtcactgtt tatggcagca cagcccgaca atctgctact ggcgaccgcg
    50641 ccgcgctact gccagtacta caatcaactc caccaactgc cgttggttgc tcttcctctc
    50701 ccgtttgatg aaagccagca aaaaaagctg gaagtccctt ttacccttct gtggcataaa
    50761 cggaacagcc gtaatccgaa gatcgtctgg ttacgggaaa ccattaaaaa tctttacgcg
    50821 tcgatggcat aaccgaatcg tatgaaacgc gacccaattt cacaatgaaa tgtaaaaaag
    50881 ttgtaataaa cttatctgaa tcgaactttt agccgcttta gtctgtccat cattccagta
    50941 aatgattact cttgtattca taatggacca ttaagcatgg agcgaaaaat ggcgactcac
    51001 tttgcccgag ggattttaac ggaaggacat ctgatttctg ttcgtctccc ctccctgtgt
    51061 catcaagaag cccgaaacat tccccctcat cgtcaaagcc gttttctggc gtccagaggt
    51121 ttactcgcag aactgatgtt catgctgtat ggcattggcg aattgccgga aatcgtcacc
    51181 ctgccgaaag gaaaaccggt tttcagcgat aaaaatttgc cttcgttttc tatttcctat
    51241 gccgggaata tggtcggcgt ggcgttaaca accgaaggtg aatgtggcct cgatatggaa
    51301 ctacagcgtg cgacacgcgg gtttcatagc ccacacgcgc ccgataacca caccttttcc
    51361 agcaatgaat cgctatggat cagtaaacaa aacgatccta acgaagcgcg ggcgcagctc
    51421 atcacgctgc gccgaagcgt gctaaaacta accggtgatg ttttgaatga cgatccgcgc
    51481 gatctgcaac tactgcccat tgccggacgc ctgaaatgtg ctcatgtaaa tcatgtagaa
    51541 gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg cggtcacgcc cacgattgaa
    51601 aagctcagtg tctgggagtt agatggcaaa cacggctgga aaagcctgcc cgatattcac
    51661 agccgcgcca acaacccgac cagccggatg atgcgttttg cccagctctc taccgtgaag
    51721 gctttttcgc caaattgata gacaaccaca ggagtcatca tgtctgaaaa attgcaggtg
    51781 gttacgttac tggggagcct gcgcaaaggc tcatttaatg gcatggttgc acgtaccctg
    51841 ccgaaaattg ctccggcgag catggaaatc aatgcgttac cttccattgc cgacattccc
    51901 ctgtatgacg ctgacgtaca gcaggaagac ggttttccag caacagttga agctctggcg
    51961 gaacagatcc gtcaggctga cggtgtggta atcgtcacgc cggaatataa ctactcggta
    52021 ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc tgccggatca accgctggca
    52081 ggtaaaccgg tattgattca gaccagctca atgggcgtga ttggcggcgc gcgctgtcag
    52141 tatcacctgc gccagattct ggttttcctc gatgcaatgg tgatgaacaa gccagaattt
    52201 atgggcggcg tgattcagaa caaagttgat ccgcaaaccg gggaagtgat tgatcagagt
    52261 acgctggacc acctgaccgg gcaattgacc gcatttggtg agtttattca gcgagttaag
    52321 atctaaataa aaaacccgcc agcaatcatg catggcgggt ttttaacgcg ctatcgattt
    52381 tagtgagcgt cgataaagac aatcttcagg ataaacagca gcgcaacgat gattacgcac
    52441 gggctaaggt cacgcagacg tccggtacca atcttcatca cgcagtagga gataaagccc
    52501 agcgcgatac cttcagtaat cgagaagctg aacggcatca tcacagcggt aataaacgcc
    52561 ggaacagatt cagtaagatc ctgccagttc acgcgagcaa gacttgaggt catcaacacg
    52621 ccaacgtaaa tcagcgcacc agctgcagcg tagcctggca ccatccccgc caacggcgac
    52681 aggaagataa ccagcaggaa cagcaaacca acaaccactg ccgtcagacc ggtacgaccg
    52741 ccaacggaaa cgccggaaga agattcaata tacgccgtaa cagaagaagt accgataaac
    52801 gaaccggtca ccgaagagat actgtcgaca tacagcgcct gcttcatgcg cgggaattta
    52861 cctttctcat cagccagacc tgctttatcg gtcacgccaa tcagcgtacc ggaggagtca
    52921 aacaagttga ccagcatgaa agagaaaatc acccctgcca gcccaaggtt aaacgacccg
    52981 gctaaatcta catgacccac gacactcatt acactcggcg gcgcagaaac gatgccatta
    53041 tagtggacat cacccagcat ccagcccagc agcgttgtca ccacgatgga aaccagcacc
    53101 gccgcgtgaa tattgcgcga ggccagaatg gcgatgatga agaagccgag gatccccagc
    53161 agcacgctgt gagaagtcag attaccgatg ctcaccagcg tttccgggtt agcgacaatc
    53221 acacctgcgt ttttcagccc catcatgcca ataaacagac cgataccact ggtgatgcca
    53281 acacgtagac ttaccgggat gttggctatc atccagtagc gaacgcggaa aatcgtcagt
    53341 aacagcagac caatcgcacc ccagaagatt gcgcccatcc cgacttgcca cggcaagccc
    53401 atcgcctgta caacgacaaa agcgaagaac gcattcaggc ccattgccgg tgccagtgca
    53461 actggcaggt tagcaaacaa ccccatcata atactgccga atgcagcaat cagacaggtc
    53521 gtgacgaaga cggcgctggt atccatgcca gcaacgccaa gaatttgcgg gttaacaaaa
    53581 acgatgtaaa ccatcgtcag gaaggtggta aaaccggcga tcacttcggt ccgtgccgtc
    53641 gtgccatgtt cgcgcagttt aaacacgcgt tccagcatcc cctggccaga agcctgggtt
    53701 gtgtgttgtt gactcattat ctatttccga acaaggaggg aaaatccgtc gctatcgtat
    53761 accaaaatga gacaatatgg acggctgaag ataacttttt ttcattgtat ttgcttatac
    53821 ggcaacgatt gcgtcatgcg aattggcatg acgaaaacgt taaactaacg ataaaggaaa
    53881 gacatgtccc agatagaagc ggtatttttc gactgcgacg gtacgctggt cgatagcgag
    53941 gtcatttgct ctcgcgccta tgtcacgatg ttcagggaat ttggcattca cgttgacctg
    54001 gaagagattt ttacccgttt taaaggcgta aaactctacg aaatcatcga catcatcagt
    54061 aaagaacagg gcgtgacgat ggtccaggcc gatgccgagc acgtttaccg cgcagaagta
    54121 gcacgtctgt ttgataccga acttgaggac atcgcaggag cgaacgaatt gctggcaagc
    54181 attaacgtgc cgatgtgcgt cgtctctaac ggtccggtca gcaaaatgca gcattcgctt
    54241 ggtaagctca acatgctgca ctacttcccg gaaaaactgt tcagcggcta tgatatccag
    54301 cgttggaagc ccgatccggc attaatgttc catgcggcaa aagcgatgaa tgtgaacgtt
    54361 gaaaactgca ttctggtcga tgactccagc gccggtgcgc aatcagggat agatgccgga
    54421 atggaagtgt tttacttctg cgctgacccg cacaataaac cgatcgttca cccgaaagtc
    54481 accaccttta cccatctttc gcaattaccg gaactgtgga aggcgcgtgg ttgggatatt
    54541 acggcatagt tattcacact cccttcactt actccactta aattggcacg caaaggtaag
    54601 taaaggga
//
LOCUS       DAZGJT010000020        54460 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZGJT010000020 DAZGJT010000000
VERSION     DAZGJT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.020"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..217
                     /locus_tag="ACHKFK_001330"
     CDS             <1..217
                     /locus_tag="ACHKFK_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051706.1"
                     /translation="KVAMLMQAPYYFQEAQIEAAIATMDVAPEYADIRQVESSTAVLY
                     LFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            281..949
                     /gene="ydhX"
                     /locus_tag="ACHKFK_001331"
     CDS             281..949
                     /gene="ydhX"
                     /locus_tag="ACHKFK_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO9051707.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVIDNDNGTQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAETRLA
                     KGFPPICVSACPEHALIFGHEDSPEIQAWLQENKYYQYQLPGAGKPHLYRRFGRHLIK
                     KENV"
     gene            946..1731
                     /gene="phsC"
                     /locus_tag="ACHKFK_001332"
     CDS             946..1731
                     /gene="phsC"
                     /locus_tag="ACHKFK_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO9051708.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPQFWPVWLIIAGLLLVGMWLVL
                     GLHALLRARGVKKSATDHGERIYLYSKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLDRAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNLLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGEMFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            1735..2547
                     /gene="ydhT"
                     /locus_tag="ACHKFK_001333"
     CDS             1735..2547
                     /gene="ydhT"
                     /locus_tag="ACHKFK_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO9051709.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGERLFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGARARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANCGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGCAALAYHDGERVRFAVAIEGENNIRAGVRYRLNEQHQFVEC"
     gene            complement(2559..4163)
                     /gene="ydhS"
                     /locus_tag="ACHKFK_001334"
     CDS             complement(2559..4163)
                     /gene="ydhS"
                     /locus_tag="ACHKFK_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO9051710.1"
                     /translation="MKTIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDDENSKLMLANIASIEIPPIYCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMIATNQDLPGETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGILGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVIFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKRENHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYEMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(4289..4594)
                     /gene="ydhR"
                     /locus_tag="ACHKFK_001335"
     CDS             complement(4289..4594)
                     /gene="ydhR"
                     /locus_tag="ACHKFK_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO9051711.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFSDEKSALTYLEKHTARLKNLGVEEVVAKVFDVNVSLSQFNQAKLA"
     gene            complement(4702..4778)
                     /locus_tag="ACHKFK_001336"
     tRNA            complement(4702..4778)
                     /locus_tag="ACHKFK_001336"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4742..4744),aa:Val,seq:gac)
     gene            complement(4783..4859)
                     /locus_tag="ACHKFK_001337"
     tRNA            complement(4783..4859)
                     /locus_tag="ACHKFK_001337"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4823..4825),aa:Val,seq:gac)
     gene            5167..6423
                     /gene="ydhQ"
                     /locus_tag="ACHKFK_001338"
     CDS             5167..6423
                     /gene="ydhQ"
                     /locus_tag="ACHKFK_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO9051712.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCAQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDKGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKANDTLLAGGTMNNLGGEDSDTIVENGAIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFGIGNINL
                     NGGKLWLITDAATQVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNSLKLQPDQDGIGATLQPA"
     gene            complement(6464..7837)
                     /gene="mdtK"
                     /locus_tag="ACHKFK_001339"
     CDS             complement(6464..7837)
                     /gene="mdtK"
                     /locus_tag="ACHKFK_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO9051713.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSAI
                     ILQRASR"
     gene            8052..8693
                     /gene="ribE"
                     /locus_tag="ACHKFK_001340"
     CDS             8052..8693
                     /gene="ribE"
                     /locus_tag="ACHKFK_001340"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO9051714.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(8733..9881)
                     /gene="cfa"
                     /locus_tag="ACHKFK_001341"
     CDS             complement(8733..9881)
                     /gene="cfa"
                     /locus_tag="ACHKFK_001341"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO9051715.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(10172..11383)
                     /gene="punC"
                     /locus_tag="ACHKFK_001342"
     CDS             complement(10172..11383)
                     /gene="punC"
                     /locus_tag="ACHKFK_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO9051716.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLFIGLTIFALGSLGMLWVENAATLLILRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVGLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            11496..12428
                     /gene="punR"
                     /locus_tag="ACHKFK_001343"
     CDS             11496..12428
                     /gene="punR"
                     /locus_tag="ACHKFK_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO9051717.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(12425..13450)
                     /gene="purR"
                     /locus_tag="ACHKFK_001344"
     CDS             complement(12425..13450)
                     /gene="purR"
                     /locus_tag="ACHKFK_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO9051718.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQSHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            13749..13838
                     /gene="cydH"
                     /locus_tag="ACHKFK_001345"
                     /gene_synonym="ynhF"
     CDS             13749..13838
                     /gene="cydH"
                     /locus_tag="ACHKFK_001345"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO9051719.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            14004..15173
                     /gene="ydhP"
                     /locus_tag="ACHKFK_001346"
     CDS             14004..15173
                     /gene="ydhP"
                     /locus_tag="ACHKFK_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051720.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPAFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAMSMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPEAVCVANS"
     gene            complement(15319..15900)
                     /gene="sodB"
                     /locus_tag="ACHKFK_001347"
     CDS             complement(15319..15900)
                     /gene="sodB"
                     /locus_tag="ACHKFK_001347"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO9051721.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(16028..16855)
                     /gene="mepH"
                     /locus_tag="ACHKFK_001348"
     CDS             complement(16028..16855)
                     /gene="mepH"
                     /locus_tag="ACHKFK_001348"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO9051722.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTQKTQKASQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            17190..17537
                     /gene="grxD"
                     /locus_tag="ACHKFK_001349"
     CDS             17190..17537
                     /gene="grxD"
                     /locus_tag="ACHKFK_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO9051723.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(17754..17816)
                     /locus_tag="ACHKFK_001350"
                     /pseudo
     CDS             complement(17754..17816)
                     /locus_tag="ACHKFK_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
     gene            complement(17909..18556)
                     /gene="rnt"
                     /locus_tag="ACHKFK_001351"
     CDS             complement(17909..18556)
                     /gene="rnt"
                     /locus_tag="ACHKFK_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO9051724.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(18659..19066)
                     /gene="gloA"
                     /locus_tag="ACHKFK_001352"
     CDS             complement(18659..19066)
                     /gene="gloA"
                     /locus_tag="ACHKFK_001352"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO9051725.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTNVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(19147..20244)
                     /gene="nemA"
                     /locus_tag="ACHKFK_001353"
     CDS             complement(19147..20244)
                     /gene="nemA"
                     /locus_tag="ACHKFK_001353"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO9051726.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSIPRALELGEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(20281..20880)
                     /gene="nemR"
                     /locus_tag="ACHKFK_001354"
     CDS             complement(20281..20880)
                     /gene="nemR"
                     /locus_tag="ACHKFK_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO9051727.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            20983..21222
                     /gene="ydhL"
                     /locus_tag="ACHKFK_001355"
     CDS             20983..21222
                     /gene="ydhL"
                     /locus_tag="ACHKFK_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO9051728.1"
                     /translation="MTEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRVNKPAPSDEPEQPSLF"
     gene            21271..22167
                     /gene="ydhF"
                     /locus_tag="ACHKFK_001356"
     CDS             21271..22167
                     /gene="ydhF"
                     /locus_tag="ACHKFK_001356"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO9051729.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTACEENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRIRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            22248..22769
                     /gene="sodC"
                     /locus_tag="ACHKFK_001357"
     CDS             22248..22769
                     /gene="sodC"
                     /locus_tag="ACHKFK_001357"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9051730.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEVKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(22770..24782)
                     /gene="ydhK"
                     /locus_tag="ACHKFK_001358"
     CDS             complement(22770..24782)
                     /gene="ydhK"
                     /locus_tag="ACHKFK_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9051731.1"
                     /translation="MNASSWSLRNFPWFRATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWRPETTDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPDATREIL
                     EQLLTALASSQTDVYTVARIISPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLPRTSGLARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMCTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLALA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(24782..25639)
                     /gene="ydhJ"
                     /locus_tag="ACHKFK_001359"
     CDS             complement(24782..25639)
                     /gene="ydhJ"
                     /locus_tag="ACHKFK_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416161.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO9051732.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIIELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVDAAQATLKQAQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFTLDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(25642..25878)
                     /gene="ydhI"
                     /locus_tag="ACHKFK_001360"
     CDS             complement(25642..25878)
                     /gene="ydhI"
                     /locus_tag="ACHKFK_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO9051733.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVIHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIVW"
     gene            26079..26513
                     /gene="slyA"
                     /locus_tag="ACHKFK_001361"
     CDS             26079..26513
                     /gene="slyA"
                     /locus_tag="ACHKFK_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO9051734.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLIKLIAKLEHNIIELQAKG"
     gene            complement(26560..27027)
                     /gene="slyB"
                     /locus_tag="ACHKFK_001362"
     CDS             complement(26560..27027)
                     /gene="slyB"
                     /locus_tag="ACHKFK_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO9051735.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRQVQIQGGDDSNVIGAIGGAVLGGFLGNTIGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTHFSPGQRVVLASNGSQVTVSPR"
     gene            27301..28410
                     /gene="anmK"
                     /locus_tag="ACHKFK_001363"
     CDS             27301..28410
                     /gene="anmK"
                     /locus_tag="ACHKFK_001363"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO9051736.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLCDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            28508..28837
                     /gene="mliC"
                     /locus_tag="ACHKFK_001364"
     CDS             28508..28837
                     /gene="mliC"
                     /locus_tag="ACHKFK_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO9051737.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVERMQTDTLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGEEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            28896..29552
                     /gene="pdxH"
                     /locus_tag="ACHKFK_001365"
     CDS             28896..29552
                     /gene="pdxH"
                     /locus_tag="ACHKFK_001365"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO9051738.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHDQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            29681..30955
                     /gene="tyrS"
                     /locus_tag="ACHKFK_001366"
     CDS             29681..30955
                     /gene="tyrS"
                     /locus_tag="ACHKFK_001366"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO9051739.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            31017..31877
                     /gene="pdxY"
                     /locus_tag="ACHKFK_001367"
     CDS             31017..31877
                     /gene="pdxY"
                     /locus_tag="ACHKFK_001367"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HFO9051740.1"
                     /translation="MKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNHT
                     QYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKAA
                     NPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHPVNNV
                     EEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQP
                     VGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIAN
                     PEHYFSATKL"
     gene            complement(31921..32526)
                     /gene="gstA"
                     /locus_tag="ACHKFK_001368"
     CDS             complement(31921..32526)
                     /gene="gstA"
                     /locus_tag="ACHKFK_001368"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO9051741.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDNYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKSTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(32631..34133)
                     /gene="dtpA"
                     /locus_tag="ACHKFK_001369"
     CDS             complement(32631..34133)
                     /gene="dtpA"
                     /locus_tag="ACHKFK_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO9051742.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHTILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLFTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(34744..35379)
                     /gene="nth"
                     /locus_tag="ACHKFK_001370"
     CDS             complement(34744..35379)
                     /gene="nth"
                     /locus_tag="ACHKFK_001370"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO9051743.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(35379..36074)
                     /gene="rsxE"
                     /locus_tag="ACHKFK_001371"
     CDS             complement(35379..36074)
                     /gene="rsxE"
                     /locus_tag="ACHKFK_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO9051744.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDEKMKKRRTEAV
                     AERALPNGETGNV"
     gene            complement(36078..36698)
                     /gene="rsxG"
                     /locus_tag="ACHKFK_001372"
     CDS             complement(36078..36698)
                     /gene="rsxG"
                     /locus_tag="ACHKFK_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO9051745.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDANWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(36702..37760)
                     /gene="rsxD"
                     /locus_tag="ACHKFK_001373"
     CDS             complement(36702..37760)
                     /gene="rsxD"
                     /locus_tag="ACHKFK_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO9051746.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDTIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEEIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPDTLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(37761..39983)
                     /gene="rsxC"
                     /locus_tag="ACHKFK_001374"
     CDS             complement(37761..39983)
                     /gene="rsxC"
                     /locus_tag="ACHKFK_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO9051747.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDNVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSHDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEQQVDPRKAAVEAAIARAKARKLEQQQANAEPEQQVDPRKVAVE
                     AAIARAKARKLEHQQANAEPEEQIDPRKAAVEAAIARAKARKLEQQQANAEPEEQIDP
                     RKAAIEAAIARAKARKLEQQQANAEPEEQIDPRKAAVAAAIARVQAKKAAQQKVVNED
                     "
     gene            complement(39976..40554)
                     /gene="rsxB"
                     /locus_tag="ACHKFK_001375"
     CDS             complement(39976..40554)
                     /gene="rsxB"
                     /locus_tag="ACHKFK_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO9051748.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(40554..41135)
                     /gene="rsxA"
                     /locus_tag="ACHKFK_001376"
     CDS             complement(40554..41135)
                     /gene="rsxA"
                     /locus_tag="ACHKFK_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO9051749.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(41212..41652)
                     /gene="ydgK"
                     /locus_tag="ACHKFK_001377"
     CDS             complement(41212..41652)
                     /gene="ydgK"
                     /locus_tag="ACHKFK_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO9051750.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(41738..41953)
                     /gene="ydgT"
                     /locus_tag="ACHKFK_001378"
     CDS             complement(41738..41953)
                     /gene="ydgT"
                     /locus_tag="ACHKFK_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO9051751.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWRYVQ"
     gene            complement(42226..42351)
                     /gene="blr"
                     /locus_tag="ACHKFK_001379"
     CDS             complement(42226..42351)
                     /gene="blr"
                     /locus_tag="ACHKFK_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO9051752.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSTTVQHK"
     gene            42594..43634
                     /gene="ydgJ"
                     /locus_tag="ACHKFK_001380"
     CDS             42594..43634
                     /gene="ydgJ"
                     /locus_tag="ACHKFK_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9051753.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(43670..44671)
                     /gene="add"
                     /locus_tag="ACHKFK_001381"
     CDS             complement(43670..44671)
                     /gene="add"
                     /locus_tag="ACHKFK_001381"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO9051754.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVADLAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(44775..45947)
                     /gene="malY"
                     /locus_tag="ACHKFK_001382"
     CDS             complement(44775..45947)
                     /gene="malY"
                     /locus_tag="ACHKFK_001382"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO9051755.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLIHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDPQSVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLKAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSGRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKIAIMPGYTYGEEGRGFIRLNAGCPRSKLEKGVAGLINAIRAVG"
     gene            complement(45957..47549)
                     /gene="malX"
                     /locus_tag="ACHKFK_001383"
     CDS             complement(45957..47549)
                     /gene="malX"
                     /locus_tag="ACHKFK_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO9051756.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYIIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMVGLMRTVQA"
     gene            47724..48752
                     /gene="malI"
                     /locus_tag="ACHKFK_001384"
     CDS             47724..48752
                     /gene="malI"
                     /locus_tag="ACHKFK_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO9051757.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRIAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            48864..49631
                     /gene="hdhA"
                     /locus_tag="ACHKFK_001385"
     CDS             48864..49631
                     /gene="hdhA"
                     /locus_tag="ACHKFK_001385"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO9051758.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGKAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            49860..50450
                     /gene="uidR"
                     /locus_tag="ACHKFK_001386"
     CDS             49860..50450
                     /gene="uidR"
                     /locus_tag="ACHKFK_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO9051759.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            50838..52649
                     /gene="uidA"
                     /locus_tag="ACHKFK_001387"
     CDS             50838..52649
                     /gene="uidA"
                     /locus_tag="ACHKFK_001387"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO9051760.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVGANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYLLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            52646..54019
                     /gene="uidB"
                     /locus_tag="ACHKFK_001388"
     CDS             52646..54019
                     /gene="uidB"
                     /locus_tag="ACHKFK_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO9051761.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQAQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LINDITS"
     gene            54058..54460
                     /locus_tag="ACHKFK_001389"
                     /pseudo
     CDS             54058..54460
                     /locus_tag="ACHKFK_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        13821 a        13343 c        14115 g        13181 t
ORIGIN      
        1 caaagtcgcc atgctgatgc aagcgccata ttacttccag gaagcgcaga ttgaagccgc
       61 gatcgccacg atggatgttg cacctgaata tgccgatata cggcaggtgg agtcatcgac
      121 ggcagtgttg tacttgttta gcgagcgatt tatgacctat ggcaaggcgt atggcttatg
      181 cgaatggttc gaggtagagc agtttcagaa cccctgaaat ttcaggggga tgtcatggat
      241 agggtggaca gttgcagcca ctgcattagg ggataatcag atgtccttca ctcgacgcaa
      301 atttgttctg gggatgggaa cagtaatctt ttttactggt tctgcgtctt ctctgttagc
      361 gaatacgagg caagaaaagg aagttcggta tgccatgatt catgacgagt cacggtgcaa
      421 tggctgtaat atctgcgccc gcgcatgccg taaaactaac cacgtccccg ctcagggaag
      481 ccgtttatcg atagcacaca ttccagtcat cgataacgac aatggaacgc agtatcactt
      541 ttttcgccag tcatgtcagc actgcgaaga tgctccttgc atcgacgttt gcccgacagg
      601 ggcgtcgtgg cgtgatgagc aggggatcgt gcgggtagaa aaatcgcagt gcatcggctg
      661 cagttattgc attggcgctt gcccgtacca ggttcgctac cttaaccctg tcactaaagt
      721 ggcagataaa tgtgatttct gcgctgagac ccgactggca aaaggctttc cgcccatttg
      781 cgtcagtgcc tgcccggaac acgcattgat ctttggtcac gaagatagcc cagaaattca
      841 ggcgtggttg caggaaaata aatactatca atatcagcta cctggcgccg ggaaaccgca
      901 tctgtatcgc cggttcggtc gacatttgat taaaaaggaa aatgtatgaa cccgtcgcaa
      961 catgctgaac agtttcagag ccagttggcg aactatgtgc cacagttcac cccccaattt
     1021 tggccggtgt ggttgatcat tgccggacta ttgctggttg ggatgtggtt ggtgctgggg
     1081 ctacatgcct tgcttcgtgc tcgtggcgtg aagaaatcag ccaccgatca tggtgagagg
     1141 atttatcttt acagcaaagc ggtcagatta tggcactggt cgaatgcgtt actctttgta
     1201 ttgttgctgg caagtgggct gataaatcac tttgcgctgg tgggcgcaac tgcggttaaa
     1261 agtctggttg cggtgcatga agtttgcgga tttttgttac tggcatgctg gcttggcttt
     1321 gttctgatca atgccgttgg gggtaatggt caccactatc gcattcgtcg tcagggttgg
     1381 ctggatcgag cggcaaaaca aacgcgattt tatttgtttg gcattatgca gggggaagaa
     1441 catcctttcc cggcaacaac ccagtctaaa tttaatctct tacagcaggt cgcctatgtt
     1501 ggtgtcatgt atggattgct gccattgtta ctattgacgg ggctgctgtg tctctatccg
     1561 caagccgtgg gagaaatgtt tcctggcgta agatactggt tattgcaggc gcattttgct
     1621 ctggcattta taagcctctt ttttatcttc ggtcatcttt atctttgcac cacggggcgt
     1681 acgccacacg aaacctttaa aagcatggtc gatggctatc accggcacta acgaatgatc
     1741 attacacgag cagacctgcg agaatggcgt atcggagcgg tgatgtatcg ctggtttctg
     1801 cgccatttcc cacgtggcgg tagttatgcc gatattcatc atgcactgat tgaggaagga
     1861 tatacggact gggcggagag tctggttgag tatgcgtgga aaaaatggct ggcggatgaa
     1921 aacttcgcgc atcaggaagt ttcttctatg caaaaactgg ccacagatcc cggagaacga
     1981 ctattttgca gtcagtttgc ccgcagcgac gatcatgctc gcatcggctg ttgtgaggat
     2041 aacgccagaa tcgcaaccgc tgggtatgcc gcgcaaattg ccagtatggg ttatagcgtg
     2101 cggattggaa gtgtcggctt taacagtcac attggcagct ccggtgcacg cgcacgggtt
     2161 gctgtaaccg gtaattcttc gcggataagc agcgctggag atagcagtcg gattgcgaat
     2221 accggaatgc gagtacgcgt ttgtacattg ggtgaacggt gtcatgtcgc cagtaatggt
     2281 gacctggtgc aaatcgccag ctttggcgca aacgccagaa ttgccaactg cggcgacaat
     2341 gtacacatca ttgccagcgg tgaaaattca acagtagtga gcacgggcgt ggtcgactca
     2401 attattctcg ggccaggggg atgcgccgca ctcgcatatc atgacggcga aagagttcgc
     2461 ttcgccgtcg ccattgaagg tgaaaacaat attcgagccg gagtccgcta tcgtcttaat
     2521 gaacaacatc agtttgttga gtgctgatag ctttaccttt aatcaaacga aagacgccga
     2581 cgcgcacggg atgcaggttt tacgaccgcc cgagccatcg cctctccaat ttctgcacat
     2641 gccgtaagtc cctgaacgaa aggctggtcg tgcatcaacc agggcaacgc gccgaacgct
     2701 acgcgcccac gaatatcttc gggttgctgt aacgtataat cttcgccaac atcagggatt
     2761 tcatcccctg ttttctgtaa ttgttcgcgt agtccaggga aaggaatatc tttcacttta
     2821 agcggacgct gcccgcgggc atcaataaaa acgtcaaacg agtaactgtt gtcttccgtt
     2881 ttcaggacgg tgcgcgactc gttaatttcc atttcgtagt cttcaccgag agcgagaata
     2941 tgaatgattc ccgcttcacg taaggccaat agtcgacgaa tagactctga agggattgcc
     3001 gcataattat cgatgaaaac ccgggcaagg cctttactga accgtttatg gtcgtgttca
     3061 ttcagatgtg gaacaatttc ctgtacggct tcatgcaggc gcagaatgac ataacgccag
     3121 ggaacagtat gattttctcg tttattgcgt tcgacctcct ggagattttt ttctgcccag
     3181 tcgaatgggt cgcgttgttt gcgctcggca aaccaggctt gagcaaagga atcgacattc
     3241 aggctttcta aggctatgcg ttggctccag tctggatcag caaatttgat ttcctctact
     3301 atcaatctaa atacccgatc caaaaggccc tcttcgcctt tttgaatctc agcatttaat
     3361 gcttgatcag tgacgatgtg taagggctcg tagggaatag ggcaatagaa atcggcttcg
     3421 ggtaaaatac ctgtgcgcga cattaacgta atatttagct tttcacttgc gttatcgcgg
     3481 tgaaaaatga cgtgttgttt atcatcttca atgaacgaac catgctgaat agccactgcc
     3541 attgccgcat ccagtccgct caaggatgtt cccaaaatac ccacgttaca cgcatcgacc
     3601 tttgcttcca tcaggcctga ccacgggctg ggaaaatacg ttctggttgc ttcttcttca
     3661 tcaggccaga cgtgacccgt ggcaatcacc gctaaatcaa acgtctcgcc gggtaaatcc
     3721 tgatttgtag cgatcatgac gccagcattt gtaatttgca gatcggtaac ctggcatgat
     3781 tcataaacag ccactgcaaa tttttgctgt cgtgcctggt ctactaatcg taaaaattga
     3841 tcgcggaaat attcgcccag cagaattcgc ggtaaaaact gacgatcgtg caaggtttct
     3901 tttttaacgc cataacgctg gagatggctg gcttcttgct tttgtagcca ttcgagatac
     3961 gtgcaataaa tcggcggtat ttcaatactg gcaatgtttg ccagcatcag ttttgagttt
     4021 tcatcatcac tgtatggcat cccgacaccg gcttcgtcag cctgctcgaa gatagaaata
     4081 gaaagtggcg tttgttgctg tagaagcgag aataaggtgt agatccccgt aggcccggca
     4141 cccacaatag caattgtttt cattgttact tacctgtacg cattctttcg aatttagtgt
     4201 tatcaggtaa gtttggttga gatttttaga atttgagaaa tttcagacag ggatgaaaag
     4261 ggcactaacc gcccttgatt aaaatctgtc aggcaagttt tgcctgattg aattgactca
     4321 gtgacacatt gacatcgaaa actttggcga caacttcctc aacgccaaga tttttcagtc
     4381 gggcagtatg tttttccaga taggtaaggg cgcttttttc atcagagaaa aggtagatcc
     4441 caccggcttc gtggttcttt tcactttctg tccaaacctt ccacagaaaa ccaggttcct
     4501 gattaatcga ctcagcaagc ggtttaagct gctcagccat tgcgtcgcca aatgggccat
     4561 taaaagcaaa atgaagttgt aacaaagttg ccacagcgat tctccttctg ataacgcaca
     4621 cgaagacagt gtagatcttt taacgtattg gatacagaga agaaacagag ggacaaagaa
     4681 gaagaccaga ttaagaaaat ctggtgcgtc cgagtggact cgaaccaccg acccccacca
     4741 tgtcaaggtg gtgctctaac caactgagct acggacgcag gatggtgcgt tcaattggac
     4801 tcgaaccaac gacccccacc atgtcaaggt ggtgctctaa ccaactgagc tatgaacgca
     4861 acgttgtagg tgacaacggg gacgaatatt agcggcagag tgggaaggtg gcaagaggca
     4921 aaacgtaatt ttctgcgcta tttcgaccgt ttgcagagct tttaagcaaa ttggctatat
     4981 tttgttgatt tgcaagggcg atttttattc aggatcgcat ttacatctga tacaacccaa
     5041 taatccgtgt atcagagttt aaacagagtt gtttgccacc atctcaaatc tgtcgtggat
     5101 aaatccttcg tagcctttac acttaggtag caataaaggc acacagatgc agcaaaggaa
     5161 taaatcatgg gatctgatgc gaaaaacttg atgagcgacg ggaacgtgca aattgttaag
     5221 accggcgagg ttattggcgc gacgcaactt actgaaggcg agttgattgt tgaagctggc
     5281 ggaagagccg aaaataccgt ggtcacgggg gctggctggt tgaaagtggc aaccggtggg
     5341 atagccaaat gcgcacagta cggtaacaat ggcacgctat cggtcagcga cggtgccatt
     5401 gccacagata ttgttcagtc cgagggaggc gcaattagtc tctctacgct cgctacggtt
     5461 aatggccgcc atcccgaagg tgaattcagc gttgataaag gttatgcctg cggtttgttg
     5521 ctggaaaatg gcggtaacct gcgtgtactg gaaggccatc gcgcggaaaa aattattctc
     5581 gatcaagagg gtggcctgtt ggtcaatggg acaacctcag cggtcgtggt agatgaaggt
     5641 ggtgaattgt tggtgtatcc aggtggggaa gccagcaatt gtgagattaa tcagggcggc
     5701 gtttttatgc tggcggggaa agccaatgat acgttgcttg ctggtggcac catgaataat
     5761 ctcggtggtg aagactctga cactattgtt gagaatggag ccatctatcg tctggggacg
     5821 gatggtcttc agctctacag ttccggtaag acgcaaaacc tgtccgttaa tgtgggtggt
     5881 cgggctgaag tgcatgctgg tacgctggaa aacgcggtaa tacaaggtgg gacagtgatt
     5941 ttgttgtcac ccaccagcgc ggacgaaaat tttgtcgtag aggaagatcg tgcaccggtt
     6001 gaactgaccg ggagtgttgc attactggac ggcgcttcaa tgattattgg ctatggcgca
     6061 gatctgcaac aatcaacgat tactgtacag cagggcggtg tgttgattct cgacggcagt
     6121 acggtaaaag gcgacagtgt cacttttggt attggtaaca tcaatctgaa tggcggaaaa
     6181 ctgtggctga tcactgatgc ggcaacgcag gtgcaactga aagtgaaacg cctgcgtgga
     6241 gagggagcca tttgcctgca aaccagtgcg aaagaaattt cacctgactt catcaatgtg
     6301 aaaggggaag ttaccggtga tatacacgtt gagataacag atgccagtcg gcaaactctg
     6361 tgtaactctc tgaaattaca gccagaccaa gatggcatcg gcgcaacgct ccagcctgcg
     6421 taaaaacaca ggcgcaaccc gtgaaggttg cgccttgtct ttattagcgg gatgctcgtt
     6481 gcagaatgat ggctgacggc agacgttgca ggaaccgcat acgcagcatc atcataatgg
     6541 ctgccgacgt caggccaata ataaagccta tccagaagcc tgctggtccc ataggttcaa
     6601 cgaccagatc ggtcaatgcc agaatatagc cgcttggcaa gcccagcacc cagtaagccg
     6661 taaaggtgat atagaaaatg gaacgcgtat ctttataacc acgcaaaatt ccactgccaa
     6721 tcacctggat agagtcagaa atctgatata ccgccgccag caacatcaaa tgtgcagcca
     6781 gcgttacaac ctcgggattg tcgttgtaca acagggcgat ttgctcccgc agtgaaaccg
     6841 tgaatatggc cgtcagggtt gccatacaga cacccaccat aagcccggtc cgcgcagcgg
     6901 tttgcgcatc cagcgttgag ccttgaccca gacgataacc tacgcggatg gttaccgctg
     6961 ccgccagcga catgggaagc acgaacatca gtgaactaaa gttcagggca atctgatgtc
     7021 ctgcgacatc aacaataccg agcggagaca ctaacagagc cacgacggca aacagcgtta
     7081 cttcaaagaa tagtgccagc gcaattggca aaccgagttg aatcagtcgt ttcataaccg
     7141 caggatcggg cttcgcggtg cctttttcgt tacgaatatc gcgcatcgag cgggcgcgtt
     7201 taatgtaaga aaccatggca aggaacatga cccaatacac cgccgcagta gccacgccac
     7261 aaccaactcc accgagctca ggcataccga aatgaccata aataaagata tagttaaccg
     7321 gaatgttcac cagcaggccg ataaagccca tcaccatacc cggcttggtt tttgccagac
     7381 cttcacactg gttacgggca acctggaaga aaagataacc cggcgcgccc catagcaacg
     7441 cacgcagata acccacggct ttgtccgcca gagccggatc gatgttttcc atggagcgga
     7501 taatgtagcc tgcattccac agcaccagca taatgagaac ggaaacaaaa cccgccagcc
     7561 agaaaccttg tcgcacctga tgcgcaatgc gctcacgtcg accggaacca tttaattgcg
     7621 cgataaccgg cgttaatgcc agcagcagtc cgtgaccaaa gaggatcgcc ggaagccaga
     7681 tagaagtacc gatagcgacc gccgccatgt cggtggcact atagccgccc gccatcacgg
     7741 tatcgacaaa acccatcgca gtttgggcga tttgcgcgag aatcaccggg attgctaatg
     7801 ctaataacag acgcgcttca ctgatatact tctgcacgtg aacacctttt atttgtagtt
     7861 atatgagaga ctaaaaagcc gcctgaatgg gcagcaaaaa gaagaagcag gggaaattca
     7921 gtctattgta gcgaggattt attaattctc cagtgaaaaa atagccattc ttaccccttc
     7981 actggtaagg gcgttttcca cctgctattg tgctgaacag aatgtcttaa ctgatttcag
     8041 gagttgtaag tatgtttacg gggattgtac agggcaccgc aaaactggtg tcgattgacg
     8101 agaaaccaaa ttttcgcacg catgtggtgg agttacccga ccacatgcta gacggtctgg
     8161 aaaccggtgc ttccgtggcg cataacggtt gctgcctgac cgtgacggaa attaacggca
     8221 accatgtcag ttttgacctg atgaaagaaa cgttacgcat tactaatctt ggcgatttaa
     8281 aagtggggga ttgggtaaac gttgagcgtg cggcgaaatt cagcgatgaa attggcggac
     8341 acttaatgtc aggtcatatt atgaccaccg ctgaagtagc gaaaatatta acctcggaaa
     8401 ataatcgtca gatctggttt aaagtccagg atagtcagtt gatgaaatat atcctgtaca
     8461 aaggatttat tggcatcgac ggtattagcc tgaccgtcgg cgaagtcacg ccaacgcgtt
     8521 tttgcgtcca tttaattccg gaaacactgg aacgcacgac tcttgggaag aaaaaactcg
     8581 gcgcacgcgt caacattgaa atcgatccac aaactcaggc agtggtagat acggtagaac
     8641 gtgtgctggc ggcacgagaa aatgccatga atcaaccagg tacagaagcc tgatctgaaa
     8701 tcgcccccgg agcgggggcg atagaatagc ctctagcgag cgactcgaag gccgttttcg
     8761 actccgcgag agaacaccac ctgccagagc tgaatatcac gggcgcggaa agcacctgca
     8821 caggcattca gataataggt aaacatccgt ttaaagcgtt cactgtagtt atccgcaatt
     8881 tctggccatg cggcgaggaa tcgttcatac cacgccatca acgtagtatc gtaatcagca
     8941 ccgaagttat gccagtcttc catcacaaag tggggttcgc tggactgagc tatctggcgt
     9001 acagagggca ggcaaccgtt cggaaaaata tatttattaa tccagggatc aacattcaga
     9061 tcggtttttt tcgaaccgat ggtatggagc aggaatatac cttccggttt caaattacga
     9121 tccaccaccg caaaataggt atcgtaattt ttcggtccga cgtgctcgaa catccccaca
     9181 gaaacaatac gatcaaactg gtcgttcagg tcacgataat cttgcagcaa aatggtgaca
     9241 tccaggcctt cacagcgttc ctgagccatt ttttgctgtt cggcagaaat ggtgacgccg
     9301 accacgctta cgtcataatt agacgccatg tagtgcgcca gtccacccca gccacagcca
     9361 atatccagta cgcgcatccc tggttttaac tgcaattttt cacaaatcat tttgagcttc
     9421 gcctgctggg cagattccag attatcggca tctttccagt aagcacagga atattgcatg
     9481 aagggatcaa gcatgcggct gaacaagtca ttacccaaat cgtaatgctc tttgccgact
     9541 atccaggcac gttttttact ctgcagattg aagagacgag cgccggcaat acgcagcgtg
     9601 tctttgaaat gatggggaag ttggttctcg agacctgcgc gtaagacttt gctaaaaaac
     9661 atatccagtc ggtcacattc ccaccagcca tccatataac tttcgcctaa ccccaaagaa
     9721 ccttcctgca gaacgcgttt aaaaaaatcg gggtttttga cacgaatatc cgccggggca
     9781 gaaccgttaa tggctatacc ggcacggcta agtaattcgt tggcgatacg gtaccagtta
     9841 tcatccggta cactgacttc ttctatacac gatgaactca tagtttctcc atcactggtc
     9901 ggtgatcaga accttaaaac agcgtagacg ctttttgggc tttgtgagaa atctcgcaga
     9961 aaaaaccgcg agtctataat ccgacgtaga acagagggaa aggaggaacc cttgccgaaa
    10021 tgaccatcca tagtgagtcg ggaacgatcc tgtccccgta ataacataat atttatcgta
    10081 gttttaacaa ctcgaaatta gtataggccg ctaatttcaa tcatacaaca aaattttgtt
    10141 agcagcctaa gtataagtat atcgatatag atcagtgtga ttcgctatga gcgacttcgg
    10201 cattgccatg attctggcag ccaacttctt cacaacgttg catcatgtaa cccagagcga
    10261 ccagcactac tgttgataac atcacgctgg tggtggtgag caatggcgtg ctgatactga
    10321 taagccagga aactaccaga cttgcgagga agcacagacc cagttgcaga gtgttctgca
    10381 acgctgcggc gcgaccggtt gcatgtggga agggacgcag cgcctgggcg acaacaatcg
    10441 ggtagatcgc accgttggca atcgccatca cacagaatgg gatcaggatt tcgaccagcg
    10501 acacatggct aatgaagccc gcagcccagg tcgcaatgac gctgacagca aacagtacca
    10561 gcaaccacgg taataactgc ttgccttgcc atttctgcag cgcggcgcga cagccataac
    10621 caccaatcag aaacgcgata gtttgcggga cgtaacttaa accaataact gccgggctgt
    10681 aacccatttc actaaggatg aacggtgaac cggtcagcca tgcaaaaaaa ctggctgaac
    10741 aggccgcgta tatcagcacg ttgccgcgat aggttttaga acgtagcagg tcggtaaagg
    10801 tcagaccatc ctgactattg ttacgggcct tcgtcgtggg tttgagccag aaaataggca
    10861 gaatcagccc cacggtaatg gcaaacaggg tggcgaaaat cgcctgccag gaaaaatgga
    10921 ccagcagcca gcttcctaac agaggagcca gtgccggaga cagacccacc agcggcatga
    10981 tggtcgcaaa aatacggtta actttctgtg aaggataata atctgtcact aatgcttgcc
    11041 agataaccgc cgcggcgcag acacccacag cctgtacaaa acgcaatatc agcagcgtgg
    11101 cggcgttttc tacccacagc atccccagac tacctaacgc aaaaattgtc aggccgatga
    11161 ataacaccgg tttacgacca taacggtcgg agagtggccc ccacagaagc tgggctgcgg
    11221 caaaaccggc aaggaacaga ctaaggctgg cactgacagc agacgcaggc gtttgcaggt
    11281 cggcctgtat ggcggcgaaa gcaggcagat acatatcggt tgccagaaaa ccgagtacgc
    11341 tcaaacccgc cagccagact aaaaatcttt tcccaggttg catgtatatt ctctaaatct
    11401 ctcaattaaa caccgccgca aagtgtaggg agtgatatta gtgttgtgaa acggtaatat
    11461 ttgatgattg gtttcaaaaa ttttgcaggc agactatgtg gtcagaatat tctctggaag
    11521 ttgttgatgc ggtagcgcgt aatggtagtt ttagcgccgc agcccaggag ttgcatcgcg
    11581 tcccttctgc ggtcagctat accgtgcgtc agctggaaga gtggctggcg gtgccgcttt
    11641 ttgaacgacg tcaccgtgat gtggaactga ccgctgccgg ggcgtggttt ctcaaagaag
    11701 gacgctctgt tgtcaaaaaa atgcagatca cccgccagca atgtcagcag atagcgaacg
    11761 gctggcgcgg tcagttagct atcgcagtgg ataatattgt caggccagag cgaacacggc
    11821 agatgatcgt tgatttttat cgccattttg atgatgtcga acttcttgtc tttcaggaag
    11881 tgttcaacgg tgtctgggat gcactttccg acgggcgcgt ggaactggcg attggcgcaa
    11941 cacgcgcgat tccggtaggc ggtcgttatg ccttccggga tatggggatg ttaagctgga
    12001 gttgcgttgt tgccagccac cacccgctgg cgttgatgga tggcccgttc agcgatgata
    12061 cgttgcgcaa ctggccgtca ttggtgcgcg aagacacctc gcgaacgcta cctaaacgta
    12121 ttacctggtt gctggataac caaaaaagac ttgtcgtgcc tgactgggaa tcatcggcaa
    12181 cctgtatctc ggcaggatta tgcataggga tggtgccaac acatttcgcc aaaccgtggc
    12241 ttaatgaggg gaagtgggta gcactagaac tggaaaaccc cttcccggat tcggcatgtt
    12301 gtctgacatg gcagcaaaat gatatgtcgc ctgcgttaac ctggttgctt gagtatctcg
    12361 gcgatagtga gacgctgaat aaggagtggc tgcgggagcc ggaagagact cccgcaacgg
    12421 gtgattaacg acgatagtcg cggaacgggc cgtcagccac ggagcggcgt tcaatcaacc
    12481 gcggatgcac ttcaatggac tgcggttctt cacgtttgtt gacgatacga tccaacagca
    12541 tgttgaacgc tgtttcgccc agcgaatctt ttggctgatg gatcgtggtc agcgccggcg
    12601 taaaatagcg ggcgttgcgc acgttatcat aaccgatcag cgaaacatcc tgcggcacgc
    12661 gcagacccat ttcatcagca gcacaaagtg cgcccattgc catgatatcg ccaccacaga
    12721 agacggcagt agggcgatgc gactgcgaca ggatttgctg catggcgcga taaccggatt
    12781 caggttcaaa gtcaccctgc acaatccagc tttccggcac tttgatcatc gcttcttcca
    12841 tcgccttcat aaaaccggca aggcggcctg cgccggtgtt acgttccagc gggccaggga
    12901 taacgccgat ttcgcgatga ccgcgttcaa tcagataacg cccggccatg tagccgcctt
    12961 cgaatgcgtt atcaatgacc gcatcggtga agtcagcttt tgcttcaccc cagtccatca
    13021 ccaccattgg gatatggcga tactcttcca gcatcgccag caacggctct gggtactcag
    13081 aacacatcac cagcagacca tcgacgcgtt tttgcgccat catcgacaga taagcccgct
    13141 gtttctcaag attgttccac gcattgccca gaatcagggt gtaacctttc tggaagcaat
    13201 ttttttcgac cgcttcaatg atctcggcaa aataggccgc ttcgctgctg gtcgccagca
    13261 aaccgataga cttggtgtgg ttaaccttca ggctacgcgc caccgcgcta ggggagtagt
    13321 gtaattcttt gattgctgcc cacacggcat tgcgcgtttc ttcagcgacg aaacgtgttt
    13381 tgttgatcac gtgtgacaca gttgtagtgg aaacgtttgc tcgtttcgct acatctttta
    13441 ttgttgccat tccatttcac tccagaccct aacttcagct cctgcaaatt cgcaaggtaa
    13501 acgtttgcct ttacacacct tacgcacaac aaaaatgttg cggtacgccg gaaaaacgac
    13561 acggagcgtc aggaaggggt caatggccag tacgcaaata atgtaagcgt ggaattttgg
    13621 ccgatcttga cgaaaagggg aagtagtaaa aacacttatc attataaatg acgggagatt
    13681 ttttcatcac agtgtgtaaa aatgcgtaat atcacttttt gtgtggtaat aaaaaaggag
    13741 aaaacttgat gagcaccgac cttaaatttt cactggtaac aacgattatc gtcctcggtt
    13801 tgatcgtggc cgtgggtttg actgccgcgc tgcactgatt tcctctcgag ggagcgcgtt
    13861 ctccctcgca tctccttcct gtttgttagc aaaatggcaa aagtgttttc tctttttgtt
    13921 attttcattt ttttgtgatt actgtcatgc tttcgccact ttaatgaata tcgcgtaatg
    13981 acgcgaagag gtggagataa ggcatgaaaa ttaactatcc gttgctagcg ctggcgattg
    14041 gcgcgtttgg tatcgggaca acggagttct cgccaatggg cttgttgccc gtcattgcgc
    14101 gcggtgtgga tgtctcgatt cccgctgccg gaatgttaat cagtgcttat gcagttggcg
    14161 taatggttgg cgcgccgctg atgacgcttc tactttctca tcgtgcccgc cgcagtgcgt
    14221 tgattttcct gatggcgatt ttcacgcttg gcaacgtact ttccgccatc gcgccggatt
    14281 atatgactct gatgctttca cgcattttga ccagcctgaa tcacggagca ttttttggtt
    14341 tgggttcagt cgtggccgca agcgtagtgc caaaacataa acaggctagc gcagttgcca
    14401 ctatgtttat ggggttaacc ctggcaaata tcggtggcgt gccggcggcg acctggttgg
    14461 gcgaaaccat cggctggcgg atgtcatttc tggcaacagc ggggctgggc gtgatttcaa
    14521 tggtaagtct gttcttctca ttacctaaag gtggcgcagg ggcgcgacct gaagtgaaaa
    14581 aagagctggc ggtattaatg cgtccgcagg tgctgtctgc attgctgacg acggtactgg
    14641 gcgctggtgc aatgtttacc ctctacacct atatctctcc ggtactgcaa agtattaccc
    14701 acgccacacc ggcgttcgtc acggcaatgc tggtgctgat tggtgtcgga ttttctatcg
    14761 gtaactatct cggcggcaaa ctggcagatc gttcagttaa cggcacgttg aaaggctttt
    14821 tgttgttgct gatggtgatt atgctggcaa tcccgttcct ggcccgcaat gagttcggcg
    14881 cagctatgag catggtggtg tggggagctg caacctttgc ggtcgtaccg ccgttacaga
    14941 tgcgcgtgat gcgtgtcgcc agtgaagcgc caggtctgtc ttcatcagtc aatattggtg
    15001 cctttaatct tggaaatgcg ctgggagccg ctgctggtgg tgcggtaatt tccgctgggc
    15061 tgggatacag ttttgtgccg gtgatggggg cgattgtcgc gggactggca ttattgctgg
    15121 tgtttatgtc agccagaaaa caacctgaag cagtttgcgt tgccaacagc taattaaaca
    15181 aaaacgcaga aggataacct tctgcgttac agttgctggc aacgtgcgat ttatcttgcc
    15241 gaatgcgctg ttaacgcctc atccgcgttg gcatggtaag cgaagcgcat cagataatgt
    15301 tgcatttgcc atcagttatt atgcagcgag atttttcgct acgaattccc agttcaccag
    15361 cgcccagaag tgctccagat agccaggacg tgcattgcga tagtcgatgt aataagcgtg
    15421 ttcccagaca tcaacggtca gcagcggagt cgcatcggtg gtcagtggag tacccgcgtt
    15481 agaggttgaa acgatagcca gtttgccatc gctgtttttc accagccagg tccagccaga
    15541 accaaagttt ttgatcgctg catcagtaaa ctgagctttg aaatcggcaa agctgccaaa
    15601 agatgcggcg atagcttcag cgacttttcc agtcggttcg ccacccgcgt tcggtgccag
    15661 gcagttccag tagaaagtat ggttccagac ctgagctgcg ttgttgaata cgccaccttc
    15721 agagctgcga ataatctctt ccagtgattt accttcaaac gcggtacctt tgatcaggtt
    15781 gttcaggtta gtgacataag tctgatgatg cttgccgtag tgatactcga tggtttccgc
    15841 agaaatgtgc ggtgccagag catctttagc atatggtagt gcaggtaatt cgaatgacat
    15901 tgctactctc ctttattatt aatttgcata cgtacaatag ccttattgtg cgtatggata
    15961 gggtagcaaa ttaaaaggtg agacaaaaga tgaacttacc ctgttgccgt aacaacaggg
    16021 taaagtttta gcgaagtgtt tttggggtca ttacccggcg agcgccaaca tagtggcgct
    16081 gccagtagtc ttcactgaga gaagtgattt gaatttcttg gcccgtgcgc ggtgactgaa
    16141 taaatttgcc gttgccgaca tacacgccga catgatcggc tgtaccgcgt ccctgagtac
    16201 ggaagaagac caggtcgccg tttttcagtt cactacgttc gattggcgct gcatcgcgca
    16261 ggtgatacat ttcattcgcc gtacgcggaa tacgaatttt caccaaatct ttataagcgt
    16321 aataaaccag accgctgcaa tcaaaaccgg tacgcggtga gctgccaccc caacgatatg
    16381 gcttaccaat ttgctgcatc agtttattca ttgccacttt tgtggctttt tgcaccttcg
    16441 ctttgtgcgc gtcggcaagg gttcctgacg cggcattttt gactttcaca caatgcgatt
    16501 tatagccctt acgtttggta cacttttcgg taaagctggc tgaggccgtt ttattgacgg
    16561 cgtgtttgct gcgagttttg gcgtttttag aagcggtaat ggaagatttt ttaacggaag
    16621 aagcggtttt gcttttggtc gtacttttac tggcggcttt tttcgctgtt ttctggctgg
    16681 ctttctgggt cttttgggtg gttgccgtgc ttttcttttt atctgctttt ttggtgatat
    16741 gagtggtagc agaactctcc ctggcttgct ttgaagcatg ggccataggc gttaaaggca
    16801 gggtggtaaa aagtaaagca cagagcgtga tcgaaatacg gtttatccgc gccactgagc
    16861 gtccctctgt taaggcaggt ctatcatgcc tgcgtctgat ttacaaaact tgatgattct
    16921 aatgcataga agaagagaaa gttaatagct tttattgtta aaaaatacgt ttcgtcggcg
    16981 agcgcaaaaa aatgcagcag tgaaagctaa aaatggttat aaatacgcca gtttgccgtg
    17041 catcagggaa gaaacgaata tttgaaatgc aacaaataga gcaaagggat aaaatagcaa
    17101 aagcgtgatg aaaatgttat tttccgatgc cgcactgacc cgctacaatg tcagattact
    17161 gccgtaaaga agtttgagga agcaagacaa tgagcaccac tatcgaaaaa atccaacgcc
    17221 agattgctga aaacccgatc ctgctgtaca tgaaaggttc accgaaactg ccgagctgcg
    17281 gtttctctgc ccaggcagtc caggcgcttg ccgcatgtgg cgaacgtttt gcctatgttg
    17341 atattctgca gaatccggac attcgtgcgg aactgccgaa atatgcgaac tggccgacct
    17401 tcccgcaact gtgggttgac ggcgagctgg tgggcggttg tgatatcgtg atcgaaatgt
    17461 atcagcgtgg tgaactgcag cagctgatca aagaaactgc cgctaaatac aagtctgaag
    17521 agccggacgc ggaataatcc gtcctgcaat actgacggat gcgaacgggc atccgtcagt
    17581 cctttctcta tccccaatcc agcgcttgtg gcgaacttga aaatcccgcc tcacgtaacc
    17641 gcgtaaacat cggcgtttgc cggaccggta ggtcattcac atctgttagc gtaaagcgca
    17701 gccgtggttc gcgactggac agctgtcggc tgtttaaagg cgcgaagaaa ctagtcgcgg
    17761 gtcgccggtg aaaacacgtc cggcaggggc gatgaagggt ttagattatc tgccattaac
    17821 gctattcccc gtgaatatca tgcatgatgc gtggaatagc ctcactatag atgcctgaat
    17881 cattgcatgt catcaggcat cgactcgatt acacctcttc ggcggcaggt agcggccagc
    17941 ctcccagacg tttccagcgg ttgacgattt cacaaaacag cacagcagtg cgttcggtgt
    18001 cgtacaacgc ggagtgcgcc tgggtgctgt cgaagtccat gccagcggtc tggcaagcct
    18061 ttgacaatac ggtttgtccg agtgccagcc cggccagtgc agcagtgtca aaagtggcga
    18121 aagggtggaa cgggttacgt ttcagtgagg cgcgttctgc ggcggccatc ataaagctgt
    18181 gatcgaaatt ggcgttgtgc gccaccataa tggcgcggtt acagccgctc gctttaatac
    18241 ctttacgtac aactttaaaa atttcgtgca gcgcctcgta ctcgctgacc gcgccgcgat
    18301 cgggatcgtt cggatcaatg ccattgaagg caagggcctc tggttgcaaa tttgcgccga
    18361 caaatggttc gacgtggaaa tgtaatgtgg tgtccggcat cagccagcct tgttcatcca
    18421 ttttcagggt gatggcggca atctcaagca gcgcatcggt tttggcgtta aatccggctg
    18481 tttcaacatc gatcacaaca ggataaaaac cacgaaaacg gtcgcacaga ccggtaagtt
    18541 gagcgttatc ggacatctga ttctcttaat acggataaat tgcagcgcgc attatgacaa
    18601 atattacttg tcgatgcagt aaagatgcgg gcgcgatgag ttcacgcccg gcaggagatt
    18661 agttgcccag accgcgaccg gcgtctttct cttcgattaa ttcaattttg taaccgtccg
    18721 gatcttccac aaatgcgata accgtagtac cgccttttac cggacccgct tcacgggtta
    18781 cgttaccccc gttttgacgg attttttcgc acgcttcagc ggcgttatct acgctaagcg
    18841 cgatgtgacc ataagcagtg ccgagttcgt atttatccac gccccagttg taggtcagtt
    18901 caatcactgc ttcttcggtt tccgggccgt aaccaacaaa cgccagtgag tatttgtatt
    18961 ccgggttttc gctggtacgc agcagtttca tgcccagcac gttggtataa aaatcgatgg
    19021 agcgttgcag atcgccaacg cgcagcatgg tatgaagaag acgcatcttt atatcctcaa
    19081 aatggctaac agattcaaaa agttgtttta gtatagcggc ggctttgcgc cgctcgcaat
    19141 gttggattac agcgtcgggt aatcagtgta accttcagca ccgccaccat aaaaactttc
    19201 ggcacgctgc gggttaagct cagctttgcg ctgcaagcgg gcgaccagat ccgggttcgc
    19261 aatccagtca cgaccaaatg ctaccgcatc aattaaccct ttgccgatca gcgtttcagc
    19321 tttttctact gtgtatgcac ctgcgccgat aatcggaccg tggaaacggg cgcgtacttt
    19381 ttcgcgaaac gcatcagtat atggttcacc tcccgcccaa tctggttctg acatatgaag
    19441 ataagcaatg ccgcgtttac ccagttgttc aatcagatac agtgcgtcgg cttcttcatt
    19501 cgggccgtta tccgtgttct ggaaagtacc gattggcgaa atgcgaatgc caatgcgatc
    19561 ggcaccccat tcttcaatcc cggcatcgac cacttccagc accagacgcg cgcgattttc
    19621 cacgctgccg ccgtactgat cggtacgatg gtttgaagaa ggggaaagga actgatgcag
    19681 caaataaccg tgagcagagt ggagctctac cagatcaaaa ccggcttcac gcgcgttagc
    19741 aatggcctga cggaaatcat tgacgatacc cggaatctct cccaattcaa gcgcacgcgg
    19801 tatggatgtt tcaacacgga tcgcctgacc attttcatcg cgcagagaag tacgtgttcc
    19861 cgcgctaagt gctgaaggcg ctaccggtgc ctgaccgcca ggttgcaggc tggcgtgaga
    19921 aatgcgtccg gtgtgccaca gctgtacggc catatgacca ttttcagcat gaacgccagc
    19981 ggtaattttt ttccatgcgg caatttgttc cgggctatgg atgccaggtg cacctgcgta
    20041 tccttttgcc tgggcagaaa tttgcgtggc ttcgctaata atcaaaccgg cactggcacg
    20101 ttggcgatag tattccgcca tcaacggggt agggatgtca cccggttcaa tactgcgcag
    20161 acgcgtcagc ggtgccataa aaatacgatt tgccgccgtg atcgcgccca ctttcagtgg
    20221 ggaatacagt ttttcagatg acataaaggc tcccggtcgt ctggtgataa aaaatgcctg
    20281 ctaaacggca ggcgtcgcaa taatgttttt tacatgggcc agcgcgtttt ccagtggctc
    20341 gaaactgcgc gaaattttgg cctgcagatt cgcgccaagc cacagtgcgt aaagcacttg
    20401 tgcctgttgc agcggttcgc cacaaaaggt taaacaatgg ttctcacggc cattttccag
    20461 cgcctgagag agcagggcga tcacgccgcg agccccttta tccattgcgc tgcgcatatc
    20521 ttctgacaga tcgcacactt cggcagagag ttttactgtc aggcaaccac tgatggttcc
    20581 atgttggcaa aactggttca gtgtttgctg gtaataagcc agtatgcggt cgcggtagtt
    20641 accttcgccg gattgcagca actcagtcag tcgctggtga tatgcggcgt aatggcgctc
    20701 aagcatagca acgccaaacg cttctttaga acgaaagtag tgatagaagg accctttcgg
    20761 cacttcagcg gtttttagga gttcgcttag ccccatcccg gtgaatccac gttgcaggca
    20821 aagttgctcg cccgtcgcca ggagatgttc gcgagtatca tgttcggtgt gtttgttcat
    20881 gcgttggagt gtagtagacc agtcggtcta gtgcaagcaa agttgctact ttggacatta
    20941 acagcttcaa taatggtaat aatcttatct gaggagggcg aggtgacgga gcaattagag
    21001 ttctttcccg tccagagccc gtgccgggga atttgccagt ctgacgaacg gggattttgt
    21061 cgcggctgtt ttcgtagccg ggatgagcgt tttaactgga ataagatgag cgatggcgaa
    21121 aaacaagaag tgttgcgcct ttgtcggcag cgattaatgc gcaaattacg tgtaaataag
    21181 ccagcgccat cagacgaacc ggagcaacca tcactctttt gagcctaatc ttgcgtatac
    21241 tcctctcata tctgataaga ggaagcgatt atggttcagc gtattactat tgcgccgcaa
    21301 ggcccggagt tttcccgttt tgtgatgggc tactggcgat tgatggactg gaacatgtcc
    21361 gcccgccagt tggtcagttt tattgaagag catctggatc tcggcgtgac caccgtggac
    21421 catgctgata tttatggtgg ctatcagtgc gaagcggcgt ttggcgaggc gctgaaactg
    21481 gcacctcacc tgcgtgaacg gatggaaatc gtcagtaaat gcggtatcgc gacgaccgcg
    21541 tgtgaagaaa acgtcattgg ccattacatc actgaccgcg atcacatcat taagagcgcc
    21601 gaacagtcgc taattaatct cgcgaccgat catctggatc tgctgttaat ccaccgacca
    21661 gacccgttaa tggatgccga tgaagtggcg gacgccttta aacatctgca tcagagcggc
    21721 aaagtgcgtc attttggtgt atcaaacttt acacctgcgc aatttgcgct gttgcaatca
    21781 cgcctgccat ttactcttgc cactaatcag gtggaaatat ccccggtgca tcagccgtta
    21841 ctgctggatg gcacgctcga ccaactgcaa caactgcgta ttcgtccgat ggcgtggtcc
    21901 tgccttggtg gtggtcgtct gtttaatgat gattatttcc agccgctgcg tgatgaactg
    21961 gctgtggtgg cagaggagtt aaacgcgggc tcgattgaac aggtagttta cgcctgggta
    22021 ctacgtttac cgtcgcagcc gctgccaatt atcggctcag gtaaaattga gcgcgtacgg
    22081 gcagctgtcg aagcagaaac actgaaaatg acccgtcaac aatggtttcg tatccgtaaa
    22141 gcggcactag gttacgacgt accgtaagca cttttaggaa tagccgccgt tcaaaaatgt
    22201 gtcactggtt tacacttatt caggaatgca caatgaacgg aggtcctatg aaacgtttta
    22261 gtctggccat tctggcgctg gttgttgcga ccggcgcaca agctgccagt gaaaaagtcg
    22321 agatgaacct cgtcacgtcg caaggggttg ggcagtcaat tggtagcgtc accattactg
    22381 aaaccgataa aggtctggag ttttcgcccg atctgaaagc attacctccc ggtgaacatg
    22441 gcttccatat tcatgccaaa ggaagctgcc agccagccac caaagatggc aaagccagcg
    22501 ccgcagaatc cgctggcggg catcttgatc cacaaaatac cggtaaacat gaagggccag
    22561 aaggtgccgg gcatttaggc gatctgcctg cactggtagt caataatgac ggcaaggcga
    22621 ccgatgccgt catcgcgcct cgtctgaaat cactggatga agtcaaagac aaagcgctga
    22681 tggtccacgt tggcggcgat aatatgtccg atcaacccaa accgctgggc ggtggcggtg
    22741 aacgctatgc ctgtggtgta attaagtaat taagaagcca gcgtaccttg cggcggtgct
    22801 tgctcaagtt gcgaaagcga gcagtacagc cgccagacaa ttgccgccag ttctcgggcg
    22861 gcaggttgat gatgacgggc aaggctgtcg caaattcgct gtaattcttc aagtgtggcc
    22921 gccagtgatt tttgctgaac gccacgctca ctcatcactc cccgcaacag tgaaatacag
    22981 ttatcccgta ctcgcgataa cggatcggaa cgcgattccc agtcgcgcaa ttgccagaca
    23041 acatgagaac agttcagcaa cactacaccc cagcgtaata accaacggcg agccagcgca
    23101 tcctggctgt tactcaactg actgacgtga tgataagtga gcgattcaaa ttcgctttca
    23161 ctcagtgttg gatggcggct tagttgatcg acaaaatccc ggcgtagcgc gcgaatatgg
    23221 cggcggcttt tacgagcatc cgatcctgga cgcagaatgg cgagcgctaa ccacgccaac
    23281 gcgacgccaa caatttttgc cagattatcg ttaagaaaat cagcaaaatc atacaccggg
    23341 ggattagtga cagcgataaa cgaacccata aaaacaatca gttgccccca cagtgcggca
    23401 aatttgggca tctgcaattt aagaagctgc attgtcgcca gcagtggaaa gagaaacagt
    23461 aaaaattgcc acagatcgct aatctgcacc atcaggccaa atttgaccac aaagctgaat
    23521 agcgaaagta acaccagcgt gcacatcagc agtgacaacg acttaaacgg tgccgcgacg
    23581 gcggagtaga gtacgcagct aattgctgcc agcgttaaag cattggcacc ggcatcccat
    23641 tgtgaagcaa tactccatgc gccaatcatc atcaacgtac aaaatgtacg cagcccgctc
    23701 cacatagctt cggcgttatc ggtatgacga gccaggccgg atgtccgtgg gagtctggca
    23761 tgatcatcta caccgctttg cagacgatgt aattcctgac tactttgcag ataaaggcgg
    23821 caaaaataac gtagtcgctg ccagaaggcg acgtgccggt agtcggcgac gttggtcggg
    23881 cgtagcgggg agataatacg tgcgacggtg taaacatctg tttgcgaact ggcgagcgcc
    23941 gtcagcagct gttcgagaat ttctcgcgtg gcatctggcg gtgagggcca gttgagcaac
    24001 atacgtcgca ggctggagat gacactggtc atacgtaatt gctggtggag tagcgcatta
    24061 aggcgcgcgt tttgctggcg aaaacgatag tggctccaga aagcctggat acgcagcaaa
    24121 ttcatggtca gtatctgccc aatcacccct tcatgtgctg cacgaatggc atcggttgtt
    24181 tcaggccgcc agagtaaact ggcatgttcc agtaatcggg cgtgcatgtt tttcaatgcg
    24241 gttaaaaggg ctgtagcatc ggaactgctc ggcaggatca tcatcatcat gccgccgcac
    24301 aaaataccga ctatcacctc gcaaacacgc gcctgagcga tatcccacag ctggctggcc
    24361 tcagtaatat taaccatcgg aaaggcgatg atggcagccg tgtagcccgc cagttgaaat
    24421 gcatacgcga cgttattcgt gaagtgcgca caggcccagg tacaaaagcc aagccacgcc
    24481 gacatgctca atagaaaaaa ccacggctca ttgagcgtat gtccggcaag aagcagtgcc
    24541 gcaatggctc cgagcaaact gccagcgatg cgtccgaggc ttttgctgat gacaccgcca
    24601 acggtgggaa agctaaccac cgcagccgag gtcatcgccc aatagggctc atcaagattc
    24661 aaataatagg caaccgtcag cgccagacac atggcaatgg tattgcgtaa cgcataacgc
    24721 cattgcgcca gcgtggccct gaaccaggga aaattgcgca aggaccatga cgatgcgttc
    24781 attattgacc tacggcaatg ctacaggtgg tgcccgacac cagcgtgaca tcgcctggga
    24841 ctttatccag cgtaaagcgg acgggaacgc gttgggcgag gcgaacccag gggacgttag
    24901 gtttcacatc cggaataagg cttgagtcac tttcaacgct ctgatcataa atcgcccgac
    24961 cgatactgga cacgtgaccc tgtaacgttt tgttatcact atagagggta atttgtgctg
    25021 gtgcaccttc gcggatatgg cgcaacttgg tttcttcaaa ataaccaatg acgtaaaacg
    25081 agtggctgtc gacaagggca aacagcggtt tcccggtgtc agcgtagtcg ccgatgcggg
    25141 tagtgagatt agtcacccat ccactcaccg gggcgcgaat ttccgtttgt gccagttgcc
    25201 attgtgcctg tttcagcgtc gcctgtgcgg catcaacgct ggcctgcatc gctttaacat
    25261 tgaggttagc ggtatcgagt tcttcggcag aaataaaatt ttgtgagaga tgacgacgac
    25321 gattcgcttc gttattggct tttgcgaggt cagattgcgc tttcgccagc tgagcctgtg
    25381 cgttcagctc ggcgatctga aagggcgttt tatcgatcgt gagcagaaga tcccccgcgt
    25441 taaccagctg gttgtctttt atattcagct caataatacg tcccgacacc tgtggggtga
    25501 tagaaacctg ttctgcgcgt attttcccgt cgcgtgtcca cggagattgc atgtaataat
    25561 tccacagcca ccatccggca agaaccgcaa ctaccgctac aatgattgtt gagaaatact
    25621 taattgtttt aattgacata gttaccacac aatcagtatt gccagagcaa ggcaaacgca
    25681 aatcgcaaac agcgataaat ccattaacaa gggatgccag atgtcaccag cgtagatcca
    25741 gccacgaagc aggcggtgta tgacaagcca gatgacgaat ccaaacgcga aggctttgaa
    25801 aaacggagga aagtagacgg acgcaccgaa caccagatct tgtaagggca atcctgttgc
    25861 attgagcata aacttcacaa agtagattcc tttacgacag aatatattgc gttagattaa
    25921 taaatattct ttaagtgcga aaaatttacg cgcaatttct gaaaaatatg tctgacggta
    25981 accaaatgca gcaatacatt tgttttagca atacaattgc tgcacactat tctaaaagcc
    26041 gcataatatc ttagcaagct aattataagg agatgaaatt ggaatcgcca ctaggttctg
    26101 atctggcacg gttggtgcgc atatggcgtg ctctgataga ccatcgcctg aaaccgctgg
    26161 agttaacaca aacccattgg gttacgttac acaatatcca tcagttacct ccagaccagt
    26221 cgcaaattca actggcaaaa gcgattggca tcgagcagcc atcactggtc cgtacgctgg
    26281 accaactgga agaaaaaggg ttaatttcgc gtcaaacttg tgccagcgat cgtcgggcta
    26341 aacgtattaa actgacggaa aaggcagagc cactgatcag cgaaatggaa gccgttatta
    26401 acaaaacccg cgcggaaatt ttacatggca tctccgcaga ggaactggaa caactgatta
    26461 agctcatcgc aaaacttgag cataatatca ttgagttaca ggccaaaggg tgaaatgaag
    26521 ggggcgtgtg gtcagtaacc tgaccacacg caaacttatt tagcgcgggg aaacggtcac
    26581 ctgactgcca ttgctggcca gtacgacacg ttggcctgga gagaaatgag tgttcccttg
    26641 tttctgtacc accatgatgg tgttgccatc atctttacga atttccagct cgacaccctg
    26701 cgttttgttc attgcactct gtacgccctg accggctaca ccacctgcaa cagcccctgc
    26761 tgcagtagcc agagaacgcc cggttccgcc gccaatagta tttcccagga aaccaccaag
    26821 tacagcacca ccaattgcgc cgataacgtt ggaatcatca ccgccctgaa tctgtacctg
    26881 acgtacatta acgatggtgc catagctgac attctgtact tgtttcgctt cagaagcggt
    26941 ataaacatcc cctgacaggg tgtcgttatt aacacaaccg acaagagaca gacctaccat
    27001 tgaaacaacc aatacgcgtt taatcattga aaaatctcct gttcaccatg aaacgccacg
    27061 caagcatccc tcatggtcaa agtatatggc atatgtgaat ccatggttac atgttctacc
    27121 aaatatgagc aaatcataat gaaagtagga tcaaccaatt ataaacaaaa tattcatgat
    27181 gattattgat ttgaagataa tgttttacta ccaacggtaa aaattggtca ttccgcatgg
    27241 tattcatgaa aagtttatgt ttgagtggtg cgcaaacgtc agattgaaaa aggatgaact
    27301 atgaaatcgg gccgctttat tggcgttatg tcaggcacca gccttgatgg tgttgatgtt
    27361 gtgttggcga caattgatga acaccgggtc gcacagctgg caagtttgag ctggccgatc
    27421 ccggtgtctc tgaaacaggc tgtactggat atttgccagg gccagcagct tacactttcg
    27481 cagtttggac agcttgatac tcaacttggg cgactttttg ctgatgcggt caatgccttg
    27541 cttaaggaac aaaacctgca ggcgagagat atagttgcga tcggctgcca cggtcaaacc
    27601 gtctggcacg aaccgacggg cgtggcacca cacactttac agattggcga taacaatcaa
    27661 attgtggcgc gcaccggaat tacggttgtc ggtgattttc gccgtcgcga tattgccttg
    27721 ggaggacaag gtgcaccgct ggtacctgcg ttccatcatg cgctgctggc tcacccaacc
    27781 gagcgacgaa tggtgctcaa tattggcggc atcgccaatc tgtcactgct cattcctggc
    27841 cagccggttg ggggctacga taccggtcct ggtaacatgc tgatggatgc ctggatctgg
    27901 cgtcaggccg gtaaacctta cgataaagat gccgagtggg cacgggcggg taaagttatt
    27961 ctcccactgc tgcaaaatat gctctgcgac ccatatttct cgcaacccgc accgaaaagc
    28021 accggacgcg aatactttaa ctacggctgg ctggagcgcc atttgcgcca ttttccgggg
    28081 gttgatcccc gtgatgtgca ggccacactg gcagaactca ccgccgtgac catttctgaa
    28141 caagttttgt tgagcggtgg ctgcgaacga ttgatggtat gtggtggagg tagtcgtaat
    28201 ccgctactca tggcgcgtct ggcggcatta ctgccaggca cagaagtcac caccaccgat
    28261 gccgttggca ttagtggcga tgacatggaa gcattggcgt tcgcctggct tgcctggcga
    28321 acgctggcgg gattaccagg aaatctgcct tccgtcactg gcgcaagcca ggagacggta
    28381 ctgggggcta ttttccccgc taacccgtga tatcagagtt aactgaattt ttcttaccgc
    28441 gtctgccgtt acacttggaa cgttagggag ggcgtattgc cctccagacc aggaaagtct
    28501 tcgggatatg tctatgaaaa aactgttact gatctgtttg ccggtgcttc tcaccggctg
    28561 tagcgcgttc aatcaactgg ttgagcgcat gcaaaccgat acgctggaat accagtgtga
    28621 tgaaaaaccg ttgacggtca aactgaataa tccgcgccag gaagtcagtt ttgtttacga
    28681 taatcaacta ctgcatctca aacagggaat ttcagcgtct ggtgcacgtt acaccgacgg
    28741 aatctatgtt ttctggtcga aaggcgagga agcgactgtc tataaacgcg accgcatcgt
    28801 cttgaataac tgtcagttac aaaatccgca gcgttgagat ttttccaggg gcggcgcaca
    28861 atagcgtcac ccactgacaa tccgtaaaga aaaccatgtc tgataacgac gaattgcagc
    28921 aaatcgcgca tctgcgccgt gaatacacca aaggcgggtt acgccgccgc gatctacccg
    28981 ccgatccatt aacccttttt gaacgttggc tctctcaggc ttgtgaagcc aaactggctg
    29041 accctaccgc gatggtggtc gctaccgtgg atgaacatga tcagccttat cagcgcatcg
    29101 ttttactcaa acattacgac gaaaaaggca tggtgtttta caccaacctc ggcagccgta
    29161 aagcacatca aatcgaaaat aatccgcgcg ttagcctgct gttcccgtgg catacccttg
    29221 aacgccaggt gatggtgatc ggtaaagcgg aacgactttc gactctcgaa gtgatgaaat
    29281 attttcatag ccgcccgcgt gatagccaga ttggggcatg ggtttcgaag cagtccagtc
    29341 gcatttctgc ccgcggtatc cttgaaagta aattcctgga gctgaagcag aagtttcaac
    29401 agggcgaagt gccattgcca agtttttggg gcggttttcg cgtcagcctt gaacagattg
    29461 agttctggca gggcggtgag catcgcctgc atgaccgctt tttgtaccag cgtgaaaatg
    29521 atgcgtggaa gattgatcgt cttgcaccct gaaaagatgc aaaaatcttg ctttaatcgc
    29581 tggtactcct gattctggca ctttattcta tgtctctttc gcatctggcg aaaagtcgtg
    29641 taccggcaaa ggtgcagtcg ttatatacat ggagattttg atggcaagca gtaacttgat
    29701 taaacaattg caagagcggg ggctggtagc ccaggtgacg gacgaggaag cgttagcaga
    29761 gcgactggcg caaggcccga tcgcgctcta ttgcggcttc gatcctaccg ctgacagctt
    29821 gcatttgggg catcttgttc cattgttatg cctgaaacgc ttccagcagg cgggccacaa
    29881 gccggttgcg ctggtaggcg gcgcgacggg tctgattggc gacccgagct tcaaagctgc
    29941 cgagcgtaag ctgaacactg aagaaactgt tcaggagtgg gtggacaaaa tccgcaagca
    30001 ggttgccccg ttcctcgatt tcgactgtgg agaaaactct gctatcgcgg cgaacaacta
    30061 tgactggttc ggcaatatga atgtgctgac cttcctgcgc gatattggca aacacttctc
    30121 cgttaaccag atgatcaaca aagaagcggt taagcagcgt ctcaaccgtg aagatcaggg
    30181 gatttcgttc actgagtttt cctacaacct gttgcagggt tatgacttcg cctgtctgaa
    30241 caaacagtac ggtgtggtgc tgcaaatcgg tggttctgac cagtggggta acatcacttc
    30301 tggtatcgac ctgacccgtc gtctgcatca gaatcaggtg tttggcttga ccgttccgtt
    30361 gatcaccaaa gcagacggca ccaaatttgg taaaactgaa ggcggcgcag tctggttgga
    30421 tccgaagaaa accagcccgt acaaattcta ccagttctgg atcaacactg cggatgccga
    30481 cgtttaccgc ttcctgaaat tcttcacctt catgagcatt gaagagatca acgccctgga
    30541 agaagaagat aaaaacagcg gtaaagcacc gcgcgcccag tatgtgcttg cagaacaggt
    30601 gacacgtctg gtgcatggtg aagaaggttt gcaggcggca aaacgtatta ccgaatgcct
    30661 gttcagcggt tccttgagtg cgctgagtga agcggacttc gaacagctgg cgcaggacgg
    30721 cgtaccgatg gttgagatgg aaaagggcgc agacctgatg caggcgctgg tcgattctga
    30781 actgcaacct tcccgtggtc aggcgcgtaa aactatcgcc tccaatgcca tcaccattaa
    30841 cggtgaaaaa cagtccgatc ctgaatattt ctttaaagaa gaagatcgtc tgtttggtcg
    30901 ttttaccttg ctgcgtcgcg gcaaaaagaa ttactgtctg atttgctgga aataatgcat
    30961 taagtggaaa gggggagtga gaaatcactc cccctggttt ttatacaggg aacatgatga
    31021 aaaatattct cgctatccag tctcacgttg tttatggtca tgcgggtaac agtgcggcag
    31081 agtttccgat gcgccgcctg ggcgcgaacg tctggccgct gaacaccgtt caattttcta
    31141 atcacaccca atacggcaaa tggactggct gtgtgatgcc acccagccat ttaaccgaaa
    31201 ttgtgcaagg cattgccgcc attgataaat tacacacctg tgatgccgta ttaagtggct
    31261 atctgggatc ggcggagcag ggtgaacata tcctcggtat cgttcgtcag gtgaaagcag
    31321 cgaatccaca ggcgaaatat ttttgcgatc cggtaatggg tcatccggaa aaaggctgta
    31381 tcgttgcacc gggtgtcgca gagtttcatg tgcggcacgg tttgcctgcc agcgatatca
    31441 ttgcgccgaa tctggttgag ctggaaatac tctgtgagca tccggtaaat aacgtcgaag
    31501 aagcggttct ggcagcgcgc gaactcattg cgcaaggacc acaaattgtg ttggttaaac
    31561 acctggcgcg agctggctac agccgtgacc gttttgaaat gctgctagtc accgccgatg
    31621 aagcctggca tatcagccgt ccgctggtgg attttggtat gcgccagccg gtaggtgttg
    31681 gtgatgtgac gagcggctta ctgctggtga aactgcttca gggggcaacg ctgcaggagg
    31741 ccctggaaca tgtgaccgct gcagtatacg aaatcatggt gaccacaaaa gcaatgcagg
    31801 aatatgagct gcaagtggtt gctgctcagg atcgtattgc caatccagaa cattatttca
    31861 gcgcaacaaa gctctgaaat atctcatttc ggcccacaac gacctgtggg ccgatggcta
    31921 ttactttaag ccttccgctg acagcgcgtc ttgtacttcc ggacgttcag ccatacgttg
    31981 cataaatgcc gcaatgtgct ctaacccttc cagattcagt ttcaccgcgt atgcccagcg
    32041 cagaaccgta aacagatagg catcagcaat tgtaaatcgt tgcccgcaga tccaatgctc
    32101 atccttcagt gcctcgttca catattgcag cttcttctcc agctgcgcgc gaactgtcga
    32161 tttgtactct tccggtgtat cagggcgaaa cagaggtgtg aaacctttat gcagctcggt
    32221 ggcgatgtaa ttcagccatt cgatggtttt atagcgggaa atactgttta ccggtgccag
    32281 caactggcgg tcggggacgc tgtcggcaag atactgcata atcgctacgc cttccgtcag
    32341 caaagtacca tcgtccagca gcaatgcagg tacttgtccc ttagggttaa cggcaaagta
    32401 attgtcaccg ttttcgagac gttttttcat taaatccaca ctgacgagcg taaaatcctt
    32461 tccgctctca cgcagggtga tatgggaagc gagagagcag gcgcccggtt tgtagaacaa
    32521 tttcatcggt aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa
    32581 aaaagccgct aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctacg
    32641 gctgctttcg ccgctttgtc tgcagcgtca tcctgcgtca tgcggtgcag tttcggcgcg
    32701 gtaaacagca tcagtactgc aataacggca gtagcgacac caatctgcaa gaatacgcga
    32761 ccatagactt ccagtgacat cagcggatcg gtaacgttat ccggcacagc catcatacct
    32821 gcaacataac caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg
    32881 aagcccatca gacgctgcgg aacgagttgt gcaaccattg ccagacccag gccagagatc
    32941 atcagttccc cgatactctg caggccatag ctcgcgacca gccagcttac agacacgata
    33001 ccagcgtcag acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc
    33061 accatgccga tagcaaactt ggttggcatt ggcagggtat cgcccatctt gttatagata
    33121 gcggccagaa tcgggctacc aatgatgatc cagaacgggt tcagtgcctg atactgctca
    33181 ggttctacgg ccaggcccag aatggtgtgt tcaacgttac gaatcgcaaa gaagttcagt
    33241 gacgttggca tctggctata aagcacgaag aagataatgg cttcgagcat caggatgaag
    33301 gcaacgatca ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata
    33361 accacgatac cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc
    33421 agccaggtgg cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg
    33481 tagttgatag gctcgaagtc tggttttgaa ccgtactgtt taacccaacg ttggcagaag
    33541 gcgaagttaa cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag
    33601 ccgtatttcg cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg
    33661 gacatgtagt acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta
    33721 gaaagcagag aagacgggtt agctttaaac aggccgttac cgaccgcaat agccgccata
    33781 cccatataaa cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc
    33841 agcacaatag cgccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag
    33901 ccgccgatag cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa
    33961 tccgcttcag acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg
    34021 taataaccaa aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt
    34081 ttaaaagcgt tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc
    34141 tcttttttta catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac
    34201 gcgcaattgt tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag
    34261 acaagtcctt tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg
    34321 aaatgttatc cagcgttaaa tttttcaaaa aacgtaacgg cagatttttt cactaccctg
    34381 agagggtgat gatggcttaa tggcgatatg ttctactatt tggcgttttt cctagtgaga
    34441 tagtccagtt tctgaaaaat atccagtgta atgttttgta ggtcaatatt tgttgcgtgt
    34501 gtaaatttaa atgattttaa cctgattctt gcaatgtttt tacattgcca cagcatcgtg
    34561 atcttgatca cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt
    34621 ggttatctgc aaacggatta cctgatgata acgctaagca atggcatcat tgacgttaaa
    34681 aagatgtttt aaaagaggat aaagaaaggt tatgaatggg gtaatcggtg ttacccctaa
    34741 tctttagatg tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca
    34801 gcgaggcttg cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc
    34861 gactttaaac tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt
    34921 ccccggcgca aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt
    34981 cggccagccg aatgcagtgt ttaatacgac gttggctgtt ttacgaccta caccaggcag
    35041 ggcttcaagc gcagcacgat cttccggaac ctcgccatta tgttgttcca gtaatatacg
    35101 gcaggttttg atgatatttt ctgctttgct gttataaagg ccaatcgttt tgatataggt
    35161 tttcacccct tcaacgccca gttcaagcat cgctgcaggc gtattagcca ccgggtagag
    35221 tttcgccgtc gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat
    35281 cagcaattca aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg
    35341 caggcgagtg aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg
    35401 tttggcaatg cacgttcggc aactgcttca gtacggcgct ttttcatttt ttcatcaatc
    35461 aggtattttc ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc
    35521 agcaggaaag gggagtcggt gtggaaaatc tccacgcgta atactttcgc ccagctacct
    35581 aacagcgcat ctgcaccgtc aaacaacgtg ccattgccga taatttcgcg tagtgaaccc
    35641 agcacgaaca tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc
    35701 gccggacctt ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca
    35761 atcagcggaa taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc
    35821 atctgtacag cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg
    35881 gcgggcgtcc agtgacgcag cgtcgaaatg gtcaggttgg ttagcgtcag taccagcgta
    35941 gtcgcaagtc ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagcggacaa
    36001 aggccgagca actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct
    36061 ttaatttcgc tcacgtttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac
    36121 gtctgagcgt acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc
    36181 gcgccggtga actggtcgaa atcaccacca tctttcttca ccgcccagtt cgcatcatcc
    36241 gcaccactga tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt
    36301 ttatcgccaa gccctggcgt ttcgtggtgc tcagtcacgc gcgtgccaag taccgtgccg
    36361 ttaaaatcgg ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct
    36421 tccagaacgg cggctacagg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct
    36481 ttacctaact ctggcgcagt gaccagatag caactctgtg ccagcgcatt gttatagcgt
    36541 tcggctggca gcacctgatc aaataacgcc ttttgttgca gactggcctg ttcagcaatt
    36601 gtcgttttgg tcatctggtt gatggccgca gttaaccctg tagaacccgc tgcaaatagt
    36661 gccagtgtaa tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg
    36721 tagacgcgtg gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg
    36781 gcaaaagcca cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataaaccc
    36841 gccagcgcgc cgaaaataag acgaccacga ttggtcgtag aagcagtaac cgggtcagtc
    36901 aaaataaaga atgcgccgag catggtcgca ccagacagca gatgaatttg tggtgctgcc
    36961 agagtatctg gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag
    37021 ctgaggggaa tatgccagcg aatcgctttc tgccatagca accacacgcc gccagccagc
    37081 caggcgagat ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat
    37141 tgcataatct cttcaaccga gtgaccggca cggacagagg ttttaaatgt atccagcggt
    37201 gtcgcctgac taatgccatc aatacctaag cgtagcgtgt tcatatcacc accactggcg
    37261 gtatgaccac tgaaaataac ctgtatggtg tcgataaaac cagggatgtt gaccgcaatt
    37321 tcatgtggcg gtaaccaact ggtcatctgc accgggaagg agatcagtaa gaccacataa
    37381 ccaatcattg ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg
    37441 atcaccgcaa acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt
    37501 accgccagca ataagcctgt cagcaatgct gagttatctt tcaacgttgc agctaccgac
    37561 tgcttgcgta gtttgaggac gagagcttcg gccagaagag cactgaccga cgccaacagg
    37621 atctgaacga gagtacccca accaaaaaac cacagttgtg ctgcgattcc tggcacggct
    37681 gcgagcaata ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag
    37741 ctagctattc tgaataccat ttagtcctcg tttacaacct tttgctgggc ggcttttttg
    37801 gcctgaacgc gggcaatagc cgcagcaact gccgctttgc gcggatcaat ctgttcttct
    37861 ggctctgcat tagcctgttg ctgttccagc ttgcgcgctt tggcgcgtgc aatagccgct
    37921 tcgatggcag ctttgcgcgg atcaatctgt tcttctggct ccgcattagc ctgttgctgt
    37981 tccagcttgc gtgctttggc acgggcaata gcagcttcga cggcggcttt gcgcggatca
    38041 atctgttctt ctggctccgc attagcctgt tgatgttcca gcttacgtgc tttggcgcgg
    38101 gcgatagcgg cttcgacggc gactttgcgc ggatcgacct gttgttctgg ctccgcattg
    38161 gcctgttgct gttccagctt gcgtgctttg gcacgggcga tagcggcttc gacggcggct
    38221 ttgcgcggat cgacctgttg ttctggctcc gcattggctt gttgctgttc cagcttgcgt
    38281 gctttggcgc gggcaatagc cgcttcaact gcagttttac gtggatcagc aacggttgca
    38341 gcgtcgttag tttgctgcag ttctgcctgt ttcgctctgg cttgcgcttt acgggcttcc
    38401 cgtgctgcaa taattgcact gttatccggg cgttcgcccg ctttaatcac aataggctgt
    38461 gtagcctggg cctgtttctc tttcacccgc gccagggcgg cagcaatcgc atctttatct
    38521 ttggctgcag gttgaacggc tgcgctctta tgtcgttcaa ggcgagccgc tttttcgcgc
    38581 tccagacgag cctggcgcgc ttcgaaacgc gctttggctt ctgcggcgcg cttttcttcc
    38641 tgacgaatag ccgcaatttc agctttttcc tgacggaaat attgcaccag ggggatattg
    38701 ctcgggcaaa cccacgcgca agctccacat tcaatgcagt cggcaatgtt atgcgtggtg
    38761 gctttatcgt gttgctgacc tttgctgaac cagtacagct gttgcggcaa aagatccgca
    38821 gggcaggcgt cagcacaggc gctacaacgg atgcaacttt gttcttcctg tggttcgcca
    38881 agttcattgg cagagggagc caacagacag ttggtaatct ttacgaccgg gacatccagc
    38941 cacggcaagg taaagcccat tagcgggccg cccataatca ccatttggtc ggcagagggg
    39001 cagaatccgg cgtcattcaa taaatgacgc actggcgtcc ccaaccgtgc ccagacgttg
    39061 cccgggcgag cgattgcttc gccagtcagg gtcacgacac gctcggtaat cggctcgcca
    39121 tcaataacgg cacgtttcac tgcataagcg gtgccgacgt tttgcattaa tacgccgata
    39181 tcggatgaac gcccgccatg tggaacctgc ttcccggtca gaatgtaggt taattgttta
    39241 gcaccgccag aaggatattt ggtgggaatc acccgcagag aaatatcgtg agagtctgcc
    39301 agcaccgcgc gcagcatgga aatcgcctgc ggtttgttat cttcaatacc gataagaatt
    39361 tcgcgtggct gcagaatatg cgcaaggatg cggataccct ctacgacctg agccgcacaa
    39421 tcctgcatca aacggtcatc ggcggtaatg tacggctcgc actcagcagc gttgataatc
    39481 aacgtttcaa tcttatctcc gccgccctgc aatttaacgc ctgtcggaaa tcccgcacca
    39541 cccagcccgg caacaccaaa ctgatgaatg cgctcgatta actcttcgcg actgcgagag
    39601 cgataatcgg cccagccgtc gcgcgggatc cagcagtctt cgccatcggc atcaataatc
    39661 acacttaatt cagctaaagc tgaaggatga gccgtagagt ggggcgcaat agccgtaacg
    39721 gtacccgagg tgggcgcgtg aacaggcagc attttgccgc gaccacgggt aagcggctgg
    39781 ccgcgcaata cgttatcgcc gacgctaacg cacaactcac cttcagcgcc aatatgttgt
    39841 ttcagtggaa tgacaaaacg ctgcgccagt ggtacctggc gcaggggcgt accgttggac
    39901 tgggttttca tctccggtgg atggatgccg ccgttgaaat cccagatttt attttttctg
    39961 aatgcagaga ataacttaag catggtgttc cacgggaatg atacgcacgg gaatggtgtt
    40021 cagatcccat ttccaggagt caggagtttc tgcgaccggt tgcaacgaga tgcagtgtgt
    40081 cgggcacgga tcaacacata agttgcagcc cgtacagaga tcactcatta ccgtatgcat
    40141 ggcgcgggta gcgccaacga tggcgtctac cggacacgcc tgaatacatt tagtgcagcc
    40201 aatacagtta ttttcatcaa taaccgccac catccgcgct ggcgttagct cttgcgcttc
    40261 gccatccagc ggctgcggct cgacattaag caactcggca atttttagca tcacagcttc
    40321 gccacctggg gcgcaacggt tgattttttc accgttacag ctgatggctt ccgcgtaggg
    40381 gcgacagccg ggataaccgc actgaccaca ctggctctgc ggtaagattt cgtcaatttt
    40441 ctcaacgacc ggatcgtctt ccaccgcaaa acggcgggag gcataaccca gaatggcgcc
    40501 aaacgccagg cccagcaggc tcacggcggc aacggcaatc cagatagcat tcattacaac
    40561 ttcaccaaac cactaaagcc cataaaggcc agagacataa gacctgcggt aattaacgca
    40621 atggcattac cgcgaaaagg tgccgggaca tcagccacag caaggcgttc gcggatggcg
    40681 gcgaagagca ccatcaccag cgagaaaccg acagcggcgg aaaaaccgta cagcgctgac
    40741 tgcaagaaat tgtgcccgag attgatattc agcaacgcca cgccgagcac ggcacagttg
    40801 gtggtgataa gcggcaaaaa aatccccagc agacggtaaa gtaccgggct ggttttgcgc
    40861 accaccattt cggtgaactg cacaaccaca gcaatcacca gaataaacgc cagggtgcgc
    40921 aggtaaatca gattaagtgg gatcaaaatc cacgtatcga taagccaggc gcaaatagac
    40981 gccagcgtca tcacaaacgt tgttgccagc cccatgccca tcgcggtttc cagctttttg
    41041 gaaaccccca taaacggaca gagaccgaga aacttgacca gtacaaagtt attgaccagt
    41101 acagttccga caaacagtaa caggtagtca gtcatttttc ggcctgaaat aaaaaagccg
    41161 cctattatcc gttaatccag agcaggcgac aacaggttaa ctgtaaggtt attacggatt
    41221 cacaaacgtc gctttcaccc gcgacgagcg tttgaaatag gggacgatca atgcggttgc
    41281 caacaaagta aacagcaact ggcgaacggc tatgccatct tctacgggtg aaaaagcgaa
    41341 cgcttttact gccagcaata cggagataag cagccagata atatagtgtt taggaacaca
    41401 acgccgacgt ttaaagaaag cgatggtcaa ccacagtgtg taataccaca tggcgatagc
    41461 ggtaataaaa gaaacgcccc ataagatttg tgtcgtcagt gcttgtccgc cgagcgtttg
    41521 aaatgtttgc ggagaagata acgcagcggt atacagcaac aacgccagcg tcgtactcaa
    41581 cagagcgact aaaagccagg ccaacgggcc aagcaaccag cctccaatac gttgtggcgt
    41641 tgtggtggtc ataggttctc cagaatagcg acgcaaacag acaaatctaa ttttaggggc
    41701 gttattataa ggcttttata accgatcgct actttcttta ctggacatag cgccagacgg
    41761 acttcggtac ctggccgagg tcaaacaaac gcccgccaga aaccagctct gcgcgacggt
    41821 gatcggcagc acgatacata ttgatcagtt cctgatcgtc cgtcagggtg taattaagat
    41881 gatcgtagag tttttccaga ctttcgagtg aactgatttt gcgaaatttt aataagtagt
    41941 cctgaacagt cataaaaggt ccataaaata attaatacca aaggggtagg tattattagt
    42001 gatgataaaa tttcaccaaa agtgaacgct tgtgttatta caataataag aatgtgctga
    42061 tggcaaatat tcagggatga aaaactgctc tggaaagagt aacgcgcttt actatccctg
    42121 acaatcatca acaacatcga acaagataat aaattcctgg tttaatatcc gacaagtgaa
    42181 aacatgcacc cggacgggca gcatgtcgct caacaagtgc agagcttact tgtgttgtac
    42241 tgtagtactc tgttcaggtg gctgatagtt gtcaatgtga ctcgccacgc caagaagaat
    42301 gactgaaacg acaagaacga tccaacctgt taattcaata agacgattca ttacagccca
    42361 catctctgtt gattgatcca ttaacttcag ggggtaaatg ttacttagca ataatagctc
    42421 cagcaagatt tttactgagg tttttgcgat atttagcttt tgtcgttgga aaaatcgcta
    42481 tttttttgac ttaagttaaa caacatccct tattgctggc aggttattaa actgttgagc
    42541 gaggataaag gatagtgtca aatagccatc atacttcaat gagaggcaat gacatgagcg
    42601 acaacatccg tgttgggttg attgggtatg gttatgcgag caaaaccttc catgcgcccc
    42661 tgattgcggg cacgcccggg ctggagctgg cggtaatctc cagcagcgat gaaacaaaag
    42721 taaaagctga ctggccaacg gttacggttg tctctgagcc gaagcatctg tttaacgatc
    42781 ccaacataga cctgattgtc attcctacac ccaacgatac ccattttccg ttagccaaag
    42841 cggcgcttga ggcgggtaaa catgtggtcg ttgataaacc ctttaccgtg acactgtcac
    42901 aagcgcgaga gctggatgcg ctggcaaaaa gcctggggcg agtgctgtct gtattccata
    42961 accgtcgctg ggatagcgat ttcctgacgc taaaaggttt gctcgcggaa ggcgtgctgg
    43021 gtgaagttgc ttactttgag tctcattttg accgcttccg tccgcaggtg cgcgatcgtt
    43081 ggcgtgaaca gggcggccca ggcagcggta tctggtacga tttagcaccg caccttcttg
    43141 atcaggccat tacgctattt ggtttaccgg ttagcatgac ggtagatttg gcacagttac
    43201 ggcccggagc gcagtcgacc gattatttcc acgctatctt gtcctatccg cagcggcgag
    43261 tgattttaca cggcaccatg ctggcagcag ccgagtcagc acggtatatc gtgcatggat
    43321 cccgaggcag ttatgtgaaa tatggcctcg atccacagga agaacgtctg aaaaatggcg
    43381 agcgtctgcc gcaggaagac tggggctacg atatgcgcga tggcgtactt acccgcgtgg
    43441 aaggtgagga acgtgtcgaa gaaacgctgt tgacagtgcc ggggaattat ccggcttact
    43501 atgcggctat tcgtgatgcg ttaaatggcg atggtgaaaa tccggttccg gcaagtcagg
    43561 caatccaggt aatggagttg attgagctgg gcatcgaatc cgccaaacat cgcgcgactt
    43621 tgtgccttgc gtgaaaaggg gatggtgcaa ttagcaccat ccatttttgt tacttcgcgg
    43681 cgactttttc tcgcagtgcg cgtttttcct ctgcgctgag gaaagccatt tccagaccat
    43741 taatctgtgc ctggcggatt tgctcgcggg ataacccagc agctggcgcg gcaacggtat
    43801 attcgtgaat gatatccact ccctgcacgc cgggatcgtc agtgttaatg ctggcacgaa
    43861 tgccatgctc aaggaacgtt ttcagcggat gtgcagccag atccgccacg gtgctggtct
    43921 gaatattgga ggtcagacag gattcaatac caatttgttg ctcggcgaga aaatccatca
    43981 gcgcccgatc ttcaatggct tttacgccat gtccaatacg ctccgccccc agttcacgaa
    44041 tcgcctgcca gatgctttcc ggtccggcag cttcgcctgc atggacggta atatgccagc
    44101 ccgcatcacg cgcgcggttg aaatgagaaa ggaacagact tcccgggaaa ccaagttcat
    44161 caccggctaa atcaagtgcg gtaatctggt cacggtgggc taaaaaggcc tccagctctt
    44221 gctgacaggc ggcttcgccg aaggtccggc tcataatacc gataagcttc gcctgcacac
    44281 caaaggtgcg gcaaccttca cgtacgccat cgatcaccgc ttcgacaaca cccgctacag
    44341 gcagctgatg tgccattgcc atgtagcctg gtgaaaaacg cagctcgaca tagtgcaggc
    44401 cgttacgggc tgcatcttca atgttttcaa atgccacgcg gcggcaggca tcaagagagg
    44461 cgagaacttt aacgccccag tcaagtttag tcagaaagct caccagatcg ggttcgttgg
    44521 caatgacctg aacgtgggga atcagtgttt ccagggattg tgcaggaagc gagatattat
    44581 actggcggcc aagttcaaga atggtctggg gacgaatgtt gccatcaagg tggcgatgga
    44641 tatcagttaa tggcagggtg gtatcaatca tggtcgcact ctttttctgg atataaaagt
    44701 gcgcaccatt ataaaaacaa agcagggtaa aaatccacga ttgcgcaacg attttttacg
    44761 ttgcgcaatt ggggttaacc aacagcgcgg atggcgttaa ttaatccagc cacacctttt
    44821 tccagtttcg aacgtgggca gccggcattg agtcggataa aaccacgacc ttcttcaccg
    44881 taggtatatc ccggcatgat cgcgatcttt tcttgctcga taagcgcttt ttgcaacgcg
    44941 ttgtcgtcaa tattcaacgg acgtagatca agccaggcca gataagtgga ttgcgggatc
    45001 tgccagttga gttcaggaaa cgcagcgttc attttatctg cgatatacgt caggttatct
    45061 ttcagataga cgcgtaaggc atccagccat ggcgcgcctt gctgataggc ggcgatatga
    45121 gcagttaacg ccagtaccga aggggaagaa agcccatcac gtcctttcag tgccgataaa
    45181 taggcatcgc ggccactgct attttctata atcccgtaag caccggtcag ggcgggaata
    45241 ttgaaacttt ttgatcccga cgttagcaac gcccagtctc cgcgcgccac attactccag
    45301 ggaatatgcg gctgctcgcc ccaaaccata tccatatgga tttcatcgga aataacccgc
    45361 acaccatgac gctcgcacag gtcagccatg atctccagct catcgcacgt ccacactttc
    45421 ccggtaggat tctgtgggct acacaggagc ataattttac attctggttt cgctagcacg
    45481 gctttcagct tgcccatatc gcaaaaccag ccgtcagcct gcttctctaa agcaacgggc
    45541 attactgtgc gctggttacc ttcaatggcc ttgtaaaatg cgtcataggc gggtgtgtgg
    45601 atcaccacgc cttcacctgt ttcagaccac tgacgaatca gttctgaaac catatagata
    45661 acagaagggc catacaccac cgactgagga tcgatggcgg tgtaatgctg ggtggaaaac
    45721 cagtgggcaa tagccgcgag aaactcatcg tttttccagc ggctgtagcc aaacacgccg
    45781 tgtatcaggc gctgattcag cgcctcgata atgcaggggg cagtggcaaa atccatgtct
    45841 gaaatcgtga acggtaacag gtcagcagtg ccgaaacggt cagcgacata atcccactgc
    45901 gtacaccatg tgccatgacg atccacgacc tttgaaaaat cgaacatatc ttatccttat
    45961 gcctggacag tacgcatcag accgaccatt tcatctttta ctgactgaac ttgtggcccg
    46021 ataacaacct gcaggttatg ttgattaagt tgtactacgc caattgcccg attgtccttc
    46081 agtgcctgca cattaacaag cgacatatct tttacagaca aacgcagacg cgtaatgcag
    46141 ttatcgagac tgacaatatt gtcggcaccg cctaaggctt cgaggattgc cggaacgttg
    46201 taacctgatt tacccggcgc accggcaacg gctttttcga ttgaactggc aacttcacta
    46261 tcgcgccccg gggttttgag attgaagcgg gtgatagcga aacggaagat gatgtagtaa
    46321 acgacaaacc agattgccgc cactactggc accatgtacc acttggttga cagaccatgc
    46381 aaaataccga acaccacaaa gtcgatgata ttgccgtcgg tattaccgat ggtgacgccg
    46441 agcacagaca tgacagtgaa gccgaggccg gttaacagcg cgtggatgac atacagaact
    46501 ggcgctacga acaggaacag gaattccagc ggttcggtcg tgccgccaac gacacaggcg
    46561 atcaggccag aaatcagcag acctttaatt ttatggcgat tttccgggcg cgcacagtga
    46621 tacatagcta acgctgcacc tggcagaccg ccgagaaacg caggcatttt accttgcgaa
    46681 aggaaacgcg tggcgctttc agaaaaaccg tgagtggttg ggcaactcaa ttgcgcctgg
    46741 aagatggtca gtgcaccgct gacggtttga ccgcagactt cctgcgtgcc gcctgcgtcg
    46801 gtaaagcgaa ttaatgccac cagaatgtga tgcagaccaa acggcaacag cagacgttca
    46861 ccggtaccaa acagcatcgg tccgaaatct cccgcgctgt tgatcatatg gcctaagccg
    46921 ctaataccca tggcgaaaat cggccagact aatggaatca ccaggccgac aagacccatc
    46981 actagcgagg agatgattgg tacgaagcgc gtaccgccga agaatgccag cgcatccggc
    47041 aggcggatat tatggaatcg ctcatgcagc atccagacga taataccggc gatcaccgca
    47101 ccgaggatcc cggtgtcgat cgactggatc ccaagaatgc tctggatgtt attggctttc
    47161 agaaccgcgg cgtccgtggt tggcagaatg cctttattgg tcaaccagaa gtttaccgcg
    47221 aggttcatta ccgcataacc gacgaagcca gcgaatgccg ctacgccttt gttttcgcgt
    47281 gccagaccca gcgggatggc gatacagaac atgactggca ggaaactaaa agcaaacgag
    47341 ccaatcttac tcatccaggt aaagatagcc tgcaacacgg ggttgcccag gaccgggatc
    47401 agggtgatga catcatgact gctaagagaa ctaccaatgc cgagcataat gccgcagaac
    47461 gacaataatg ccacgggtaa catgaaggtt ttgcctaact gctggaagaa ctcccacagc
    47521 gtgactttcg gtgctgtttt cgccgtcata aaacgactcc tcgtagagaa caatctggat
    47581 tcagtaaatt gcgcgagaag ataaaacgtt ttatcaaatt ttagtgaggc ataaatcaca
    47641 ttacgcaacg ataatagcgg gtataagata aataaaaggt aaaacgtttt atctgtcaca
    47701 taatcaggga gtaggtcatc tgcatggcta ccgccaaaaa aataaccatt catgatgttg
    47761 cgctggctgc gggcgtgtcg gtaagcaccg tttcgctggt gctaagtggc aaagggcgaa
    47821 tctctaccgc cacaggagaa cgcgttaacg ccgccattga agagctggga tttgtgcgca
    47881 atcgccaggc gtcggcgctg cgcggcgggc aaagcggcgt cattggtttg atcgtccgtg
    47941 atttatctgc gccgttttac gccgaattga cggccggatt gacggaagct ctggaagcgc
    48001 agggacggat ggtttttttg cttcacggcg gtaaagacgg cgagcagctg gcacagcggt
    48061 tttcactatt actgaatcag ggtgtcgatg gtgtggtcat tgccggggct gcaggaagca
    48121 gcgatgacct gcgacggata gcagaagaaa aagctatccc ggtgattttc gcttcccgtg
    48181 ccagttatct tgatgatgtt gatacggttc gcccggacaa catgcaggct gcacagttgt
    48241 tgacggagca tctcattcgc aatgggcatc agcgtatcgc ctggctggga gggcaaagtt
    48301 cctcattaac ccgggcagaa cgggtggggg gctattgtgc gactctacta aaatttggcc
    48361 tgccgtttca cagcgattgg gtgctggagt gcacttccag ccagaagcaa gccgcggaag
    48421 ctatcacggc gcttttacgt cataacccga ccatcagcgc cgtggtttgc tataacgaaa
    48481 ctattgcgat gggggcatgg tttggtttgc tgaaagcagg aaggcaaagc ggggaaagcg
    48541 gagtcgatcg ttactttgag caacaggttt cgctggcggc atttaccgat gcaacgccaa
    48601 ccacacttga tgatataccc gttacctggg ccagcacgcc agcgcgggaa cttggtacca
    48661 cacttgcgga tcgcatgatg caaaaaatca cccatgaaga gacgcattca cgcaatctta
    48721 ttattcccgc ccggctcatt gcggcgaaat aatcctctct ttatctgcta tacctggtag
    48781 tgtcctttcc tcaaggttaa tgataaaaaa tgtggcacac aggcggtgcc ttttattttt
    48841 actgaagttc acaggaggtt tatgtgttta attctgacaa cctgagactc gacggaaaat
    48901 gcgccatcat tacaggtgcg ggtgcaggta ttggtaaaga aatcgccatt acattcgcga
    48961 cagctggcgc atctgtggtg gtcagtgata ttaacgccga tgcagctaat catgttgtag
    49021 atgaaattca acaactgggt ggtaaggcat ttgcctgccg ctgcgatatt acttccgaac
    49081 aggaactctc tgcactggca gactttgccg tcagtaagtt gggtaaagtt gatatcctgg
    49141 ttaacaacgc cggtggcggt gggcctaaac cgtttgatat gccaatggca gattttcgtc
    49201 gcgcttatga actgaatgta ttttcttttt tccatctgtc acaacttgtt gcgccagaaa
    49261 tggaaaaaaa tggcggtggc gttattctga ccattacttc tatggcggca gaaaataaaa
    49321 atataaatat gacctcctat gcatcatcta aagctgcggc cagtcatctg gtcagaaata
    49381 tggcgtttga cctgggtgaa aaaaatattc gggtgaatgg cattgcaccg ggggcaatat
    49441 taaccgatgc cctgaaatcc gttattacac cagaaattga acagaaaatg ttgcaacaca
    49501 caccaatcag acgtctgggc caaccgcaag atattgctaa cgcggcgctg ttcctttgct
    49561 cgcctgcagc cagctgggta agcggacaaa ttctcaccgt ctccggtggt ggggtacagg
    49621 aactcaatta atacactaac gaaccggtaa acagccgtat gtgttgttta ccgggatagc
    49681 gtgtgcaact catcaacgtc tctgctaaac aactggcagc caaatcacgg ctattggtta
    49741 accaatatct ggataaaaag cttacgaatt gagtgtgcct tcgcactatt caacagcaat
    49801 gataggcgct cacctgacaa cgcggtaaac tagttattca tgctaactat aatggtttaa
    49861 tgatggataa catgcagact gaagcacaac cgacacggac ccggatcctc aatgctgcca
    49921 gagagatttt ttcagaaaat ggatttcaca gtgcctcgat gaaagccatc tgtaaatcct
    49981 gcgccattag tcccgggacg ctctatcacc atttcatctc caaagaagcc ttgattcagg
    50041 cgattatctt acaggaccag gagagggcgc tggcccgttt ccgggaaccg attgaaggga
    50101 ttcatttcgt tgactatatg gtcgagtcta ttgtctctct cacccatgag gcctttgggc
    50161 aacgggcgct ggtggtcgaa attatggcgg aagggatgcg taacccacag gtcgccgcca
    50221 tgcttaaaaa taagcatatg acgatcacgg aatttgttgc tcagcggatg cgtgatgccc
    50281 agcaaaaagg cgagataagc ccagatatca acacggcaat gacttcacgt ttactgctgg
    50341 atctgaccta cggtgtactg gccgatatcg aagcggaaga tctggcgcgt gaagcgtcgt
    50401 ttgctcaggg attacgcgcg atgattggcg gtatattaac cgcatcctga tcctctctct
    50461 ttccgcgggc tggtgacaac tgagcccgcg tttcatatcg taatttctct gtgcaaaaat
    50521 catcctccca gggatcggag aatcccacct aaatattcat cgtagcaata tatcatgagg
    50581 gtttatcgct cccaatacgt tcgaacgaac gttcgattgc ttattttgtg gcttctgtca
    50641 acgctgtttt aaagattaat gcgatcaata tcacgctgtg ggtattgcag tttttggttt
    50701 tttgatcgcg gtgtcagttc tttttatttc catttctctt ccatgggttt ctcacagata
    50761 actgtttgca acacagaatt ggttaactaa tcagattaaa ggttgaccag tatttttatc
    50821 ttaatgagga gtcccttatg ttacgtcctg tagaaacccc aacccgtgaa atcaaaaaac
    50881 tcgacggcct gtgggcattc agtctggatc gcgaaaactg tggaattgat cagcgttggt
    50941 gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc gggcagtttt aacgatcagt
    51001 tcgccgatgc agatattcgt aattatgtgg gcaacgtctg gtatcagcgc gaagtcttta
    51061 taccgaaagg ttgggcgggc cagcgtattg tactgcgttt cgatgcggtc actcattacg
    51121 gcaaagtgtg ggtaaataat caggaagtga tggagcatca gggcggctat acgccatttg
    51181 aagccgatgt cactccgtat gttattgccg ggaaaagtgt acgtatcact gtttgtgtga
    51241 acaacgaact gaactggcag actatcccgc cgggaatggt gattaccgac gaaaacggca
    51301 agaaaaagca gtcttacttt catgatttct ttaactacgc cgggatccat cgcagcgtaa
    51361 tgctctacac cacgccgaat acctgggtgg acgatatcac cgtggtgacg catgtcgcgc
    51421 aagactgtaa ccacgcatct gttgactggc aggtgggggc aaatggtgat gtcagcgttg
    51481 aactgcgtga tgcggatcaa caggtggttg caactggaca aggcaccagc gggactttgc
    51541 aagtagtgaa tccgcacctc tggcaaccgg gtgaaggtta tctctatgaa ctgtgcgtca
    51601 cagccaaaag ccagacagag tgtgatatct acctgctgcg cgtcggtatc cggtcagtgg
    51661 cagtgaaggg cgaacagttc ctgatcaacc acaaaccgtt ctactttact ggctttggtc
    51721 gtcatgaaga tgcggatttg cgcggcaaag gattcgataa cgtgctgatg gtgcacgatc
    51781 acgcactaat ggactggatt ggggccaact cctaccgtac ctcgcattac ccttacgctg
    51841 aagagatgct cgactgggca gatgaacatg gaatcgtggt gattgatgaa actgcagctg
    51901 tcggctttaa cctctcttta ggcattggtt tcgaagcggg caacaagccg aaagaactgt
    51961 acagcgaaga ggcagtcaac ggggaaaccc agcaggcgca cttacaggcg attaaagagc
    52021 tgattgcgcg tgacaaaaac cacccaagcg tggtgatgtg gagtattgcc aacgaaccgg
    52081 atacccgtcc gcaaggtgca cgggaatatt ttgcgccact ggcggaagca acgcgtaaac
    52141 tcgacccgac gcgtccgatc acctgtgtca atgtaatgtt ctgcgacgct cacaccgata
    52201 ccatcagcga tctctttgat gtgctgtgcc tgaaccgtta ttacggttgg tatgtccaaa
    52261 gcggcgattt ggaaacggca gagaaggttc tggaaaaaga acttctggcc tggcaggaga
    52321 aactgcatca gccgattatc atcaccgaat acggtgtgga tacgttagcc ggcctgcact
    52381 caatgtacac cgacatgtgg agtgaagagt atcagtgtgc atggctggat atgtatcacc
    52441 gcgtctttga tcgcgtcagc gctgtcgtcg gtgagcaggt atggaatttc gccgattttg
    52501 cgacctcgca aggcatattg cgcgttggcg gtaacaagaa agggatcttc acccgcgacc
    52561 gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg gaccggcatg aacttcggtg
    52621 aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc tcctggcgca ccatcgtcgg
    52681 ctacagcctc ggtgacgtcg ccaataactt cgccttcgca atgggggcgc tcttcctgtt
    52741 gagttactac accgacgtcg ctggcgtcgg tgccgctgcg gcgggcacca tgctgttact
    52801 ggtgcgggta ttcgatgcct tcgccgatgt ctttgccgga cgagtggtgg acagtgtgaa
    52861 tacccgatgg ggaaaattcc gcccgttttt actcttcggt actgcgccgt taatgatctt
    52921 cagcgtgctg gtattctggg tgccgaccga ctggagccat agcagcaaag ttgtgtatgc
    52981 atatttgacc tacatgggac tcgggctttg ctacagcctg gtgaatattc cttatggttc
    53041 acttgctacc gcgatgaccc aacaaccaca atcccgcgcc cgtctgggcg cggctcgcgg
    53101 gattgccgct tcattgacct ttgtctgcct ggcatttctg ataggaccga gcattaagaa
    53161 ctccagcccg gaagagatgg tgtcggtata tcatttctgg acaattgtgc tggcgattgc
    53221 cggaatggtg ctttacttca tctgcttcaa atcgacgcgt gagaatgtgg tacgtatcgt
    53281 ggcgcagccg tcattgaaga tcagtctgca aaccctgaaa cggaatcgcc cgctgtttat
    53341 gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc gcggtcagcg cctcgtcgtt
    53401 gttctacgtg cgctatgtgt taaatgatac cgggctgttc actgtgctgg tactggtgca
    53461 aaacctggtc ggtactgtgg catcggcacc gctggtgccg gggatggtcg cgaggatcgg
    53521 taaaaagaat accttcctga ttggcgcttt gctgggaacc tgcggttatc tgctgttctt
    53581 ctgggtttcc gtctggtcac tgccggtggc gttggttgcg ttggccatcg catcaattgg
    53641 ccagggcgtt accatgaccg tgatgtgggc actggaagct gataccgtag aatacggtga
    53701 atacctgact ggcgtgcgaa ttgaagggct cacctattca ctattctcat ttacccgtaa
    53761 atgcggtcag gcaatcggag gttcaattcc tgcctttatt ttgggattaa gcggatatat
    53821 cgccaatcag gcgcaaacgc cggaagttat tatgggcatc cgcacatcaa ttgccttagt
    53881 accttgcgga tttatgctac tggcattcgt cattatctgg ttttatccgc tcacggataa
    53941 aaaattcaaa gaaatcgttg ttgaaattga taatcgtaaa aaagtgcagc agcaattaat
    54001 caacgatatc actagttaat attcaataaa aataatcaga acatcaaagg tgcaactatg
    54061 agaaaaatag tggccatggc cgttatttgc ctgacggctg cctctggcct tacctctgct
    54121 tacgcggcgc aactggctga cgatgaagcg ggactacgca tcagactgaa aaatgaatta
    54181 cgcagggctg ataagcccag tgctggcgcg gggagagata tttacgcatg ggtataggga
    54241 ggattgctcg atttcaatag tggttattat tccaatatta ttggcgttga aggcggggcg
    54301 tattatgttt ataaattagg tgctcgtgct gatatgagca cccggtggta tcttgatggt
    54361 gataaaagtt ttggctttac cctgggggca gtaaaaataa aacccagtga aagtagtctg
    54421 cttaaattag gtcgcttcgg gacggattat agttatggta
//
LOCUS       DAZGJT010000021        52816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZGJT010000021 DAZGJT010000000
VERSION     DAZGJT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.021"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..685)
                     /locus_tag="ACHKFK_001390"
     CDS             complement(<1..685)
                     /locus_tag="ACHKFK_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO9051762.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREAGVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGK"
     gene            complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHKFK_001391"
     CDS             complement(696..1697)
                     /gene="ltaE"
                     /locus_tag="ACHKFK_001391"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO9051763.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEVTQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMTGGGMRQSGILAAAGLYALKNNVARLQEDHDNAAWMAKQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSRAQLAEVAAHWRAFLAR"
     gene            complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHKFK_001392"
     CDS             complement(1734..3452)
                     /gene="poxB"
                     /locus_tag="ACHKFK_001392"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO9051764.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGIKGIRVEKASEID
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHKFK_001393"
     CDS             complement(3585..4553)
                     /gene="hcr"
                     /locus_tag="ACHKFK_001393"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO9051765.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSRWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVISGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHKFK_001394"
                     /gene_synonym="priS"
     CDS             complement(4565..6217)
                     /gene="hcp"
                     /locus_tag="ACHKFK_001394"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO9051766.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHKFK_001395"
     CDS             complement(6361..7260)
                     /gene="lysO"
                     /locus_tag="ACHKFK_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO9051767.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQKAALRVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHHQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVASSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7581..8276)
                     /gene="aqpZ"
                     /locus_tag="ACHKFK_001396"
     CDS             complement(7581..8276)
                     /gene="aqpZ"
                     /locus_tag="ACHKFK_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO9051768.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAASFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            8702..10360
                     /gene="ybjD"
                     /locus_tag="ACHKFK_001397"
     CDS             8702..10360
                     /gene="ybjD"
                     /locus_tag="ACHKFK_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO9051769.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNILIGENAWGKSSLLDALTL
                     LLSPESELYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKVISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10357..11313)
                     /gene="ybjX"
                     /locus_tag="ACHKFK_001398"
     CDS             complement(10357..11313)
                     /gene="ybjX"
                     /locus_tag="ACHKFK_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO9051770.1"
                     /translation="MNMSQLTERTFTSSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAELTFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFCS"
     gene            11464..12579
                     /gene="macA"
                     /locus_tag="ACHKFK_001399"
     CDS             11464..12579
                     /gene="macA"
                     /locus_tag="ACHKFK_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO9051771.1"
                     /translation="MKKRKTVKKRYVIAMVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDNAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKINDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12576..14521
                     /gene="macB"
                     /locus_tag="ACHKFK_001400"
                     /pseudo
     CDS             12576..14521
                     /gene="macB"
                     /locus_tag="ACHKFK_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
     gene            complement(14594..14818)
                     /gene="cspD"
                     /locus_tag="ACHKFK_001401"
     CDS             complement(14594..14818)
                     /gene="cspD"
                     /locus_tag="ACHKFK_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO9051772.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            14968..15042
                     /gene="yljB"
                     /locus_tag="ACHKFK_001402"
     CDS             14968..15042
                     /gene="yljB"
                     /locus_tag="ACHKFK_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO9051773.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            15141..15461
                     /gene="clpS"
                     /locus_tag="ACHKFK_001403"
     CDS             15141..15461
                     /gene="clpS"
                     /locus_tag="ACHKFK_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO9051774.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15492..17768
                     /gene="clpA"
                     /locus_tag="ACHKFK_001404"
     CDS             15492..17768
                     /gene="clpA"
                     /locus_tag="ACHKFK_001404"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO9051775.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     repeat_region   17981..18248
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=17981..18008
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaa"
     gene            18421..19404
                     /gene="cas1f"
                     /locus_tag="ACHKFK_001405"
     CDS             18421..19404
                     /gene="cas1f"
                     /locus_tag="ACHKFK_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001729819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endonuclease Cas1f"
                     /protein_id="HFO9051776.1"
                     /translation="MSSNYLTPSDLKTILHSKRANIYYLEKCRVQVNGGRVEYVTSEG
                     KESYYWNIPIANTTALMLGMGTSVTQAAMREFAHAGVMVGFCGTDGTPLYSANEVDAD
                     VSWLSPQSEYRPTEYLQQWVSFWFVEDKRLAAAKRFQLIRLTHIDKHWPSSKMLREHA
                     FQPDVNALHTLLNRTREEIDAAENHTQLMLVEAKLTKALYKMVSQTVGYGDFTRAKRG
                     GGIDMANRFLDQGNYLAYGLAAVAAWVTGIPHGLAVMHGKTRRGGLVFDLADLIKDAL
                     VMPQAFIAAMAGEDAQEFRQRCINIFQQADALDVMITSLQETAQALAKADQ"
     gene            19401..22634
                     /gene="cas3f"
                     /locus_tag="ACHKFK_001406"
     CDS             19401..22634
                     /gene="cas3f"
                     /locus_tag="ACHKFK_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated helicase Cas3f"
                     /protein_id="HFO9051777.1"
                     /translation="MNVLIISRCTKNARVESCRIIDQFAERTGDAAWQTVITLEGVNT
                     LRRLLRKTARRNTAVACHWLKKNGQTELMWIVGNIRRFNAQGRVPTNRTTQISLVNKE
                     ENRWQCAESIALLAAIAGLFHDFGKSGRSFQQSLTQKNTRSYQPYRHEWISLRLFQAF
                     VGDRPDDVWLAKLGELTADDESAILERLQKDTPQFSNSPFSSLQPLAKTVGWLILSHH
                     RMPENLSPQSEQSCKGCESWLEKQLNADWNAVNHRRDDWSQQDFAHVWQFPSGTPLQS
                     ASWREKARQIARRASNALGLKTYGQMEQLFPLHMARFCLMLADHYYSSSDAQQKWQDP
                     DYIVWANSDRQSGTLKQRVDEHLVGVAHHAYLLGRQLMHTRASLPAIARHKGFSQRAT
                     DVNYRWQDKAWDVARALRERSREEGFFGVNMASTGCGKTFANARIMYALADEREGCRF
                     TVALGLRTLTLQTGDALRSRLGLGEDELAVLIGSAAVNQLWQQEKIDNDCGSASQESP
                     AEEQQFVKYEGSLHTGTLEKWLKDDSKLKELVSAPILVSTIDHLISATEGVRGGKQLP
                     AMLRLLTSDLVLDEPDDFDIADLHVVCRLMNWAGMLGTRVLLSSATLPLGLIQALFAA
                     YLAGRKMWQASCGINGRPVNICCAWFDEKDADATQIYDGPGFRDAHAKFVARRAVMLA
                     EKERLHFGRVASVSSASGAIQDVTESVAQTVHTQMLKLHQAHRQRHESGKTVSLGLVR
                     FANINPLVAVTKALIVIPSPEDVCIHYCVYHSRHPLAVRSDIEKRLDRAFTRHNELDF
                     WNNEDIADALHNRPESHHLFVVLGTSVIEVGRDWDADWGIIEPSSMRSIIQFAGRIQR
                     HRRNVPTSENLVILRSNIKSLQKKAPAFCKPGFETKEHLLNSHDICELIPIADYRTIN
                     ALPRILSANNANKLAELEHSRLSAELMSSKSKHVVAAQWWRAPLSWNGWMQRQTPFRY
                     SPLPEAVFFLHMDDEESEARFYSRTGDNERKAQGNFRREEVRCASGVVCWGVMDYQHV
                     LLDLAEKQSCEIASVGEKFAEVRVPVSEEDAVDDWRYHPWLGVFRAP"
     gene            22964..24271
                     /gene="csy1"
                     /locus_tag="ACHKFK_001407"
     CDS             22964..24271
                     /gene="csy1"
                     /locus_tag="ACHKFK_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy1"
                     /protein_id="HFO9051778.1"
                     /translation="MEKTALIQFITDYIASRRQPKIDAFEKEAAKRLEQGEDASVIAL
                     ERQELEARYLPRNWLTDAAKRAGQIKLVTHGAKFSHGDSKASSFYLETSATESYLNTA
                     SLANVATDAIGNAAALDVAKLLQTDVNGDSLLASLKRGDYQALSTFAEDKVQLELWVA
                     GFSQAWTTGRPSSHKLAKQIYFPVADGYHLLCPLFSSSLAQAMYEKLTAGRFSEESKA
                     IRDARKAGKWHSQPDIRFPNLAEMHFGGTKPQNISLLNSVRGGRVWLLPSMPPAWGPL
                     DRAPQKMHSIFALRGDFNRAASGIIARMTYLLKVDTNNVHIRTVRAKYIDELIDLLFM
                     QASAFQQEKWQGWSAQSPDLPRHQQLWLDPWRSLSDETFKQEREKNDWQVTVADDFAR
                     WLNYRLKKSSFDVGAVEQKEWRSQSLFAQRMREMEAVLQEALK"
     gene            24268..25191
                     /gene="csy2"
                     /locus_tag="ACHKFK_001408"
     CDS             24268..25191
                     /gene="csy2"
                     /locus_tag="ACHKFK_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy2"
                     /protein_id="HFO9051779.1"
                     /translation="MSYLLLLPHIRIENANAVSGLTWGFPSMTHFLGYVHALSRKVVD
                     EFGVSFDGCAVVSHEQHIQAYSSGRDFQFALTRNPLTREGKTASFNEEGRMHLTVSLL
                     IECNGEITNGEYGRKVLCDYLKMLCQSHKLAGGSIVSMRDPQLFHAPEDEKQLRKIVW
                     RLMPGYALYDRSEWLAEHHQQHPDISLLDAWLDFAAIKYQAESPAEDNSAKWVYQPKP
                     MPGFLVPLMCGYQRISPVYAPGEVENARDTVTPFAFAEAVYGIGEWRGLHRTTDLQAL
                     MWRYRTTDTGYYCSATPVVDDFTFNEYDDLE"
     gene            25202..26203
                     /gene="csy3"
                     /locus_tag="ACHKFK_001409"
     CDS             25202..26203
                     /gene="csy3"
                     /locus_tag="ACHKFK_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated protein Csy3"
                     /protein_id="HFO9051780.1"
                     /translation="MAKAPVAIKTASVLAFERKLATSDAVMYAGNWQGNEWQPIEIQE
                     KAVRGTISNRLKNAIVSDPTKLDAEIQKANLQRVNVASLPVDTDTLKVVFTLRVLGNL
                     STPSVCNDMAYQEALSGVIEGYISEHGFKELTLRYALNLANGRFLWRNRIGAEQIQVK
                     VTANETSWTFNSHDFSLRQFDQGQAAVTQLAAIIEQGLSAKEWVMLTVEAQVRLGAGQ
                     EVFPSQELVLDSNSSKSRVLYQVAGIAGIHSQKIGNALRTIDTWHPKVDELGAIAVEP
                     YGSVTSRGVACRQPKEKLDFYTLLDNWVTKGMKPDVKQQHYVMAVLIRGGVFGEKSE"
     gene            26214..26768
                     /gene="cas6f"
                     /locus_tag="ACHKFK_001410"
     CDS             26214..26768
                     /gene="cas6f"
                     /locus_tag="ACHKFK_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-F CRISPR-associated endoribonuclease
                     Cas6/Csy4"
                     /protein_id="HFO9051781.1"
                     /translation="MDHYLEIRVLPDPEFSSEMLMAALFAKLHRVLGARGQGDIGVSF
                     PDVNVMPGTHLRLHGSAQALQELEASTWRKGLTDYCQCSPVTPVPEIKGWRVVSRVQV
                     KSNPQRLLRRSVKKGWLTEEQAIERLATQAEQRTDLPFLNMKSLSSQQQFKLFIRHGD
                     LLKEPVKGEFSSYGLSATATIPWF"
     gene            complement(26880..26967)
                     /locus_tag="ACHKFK_001411"
     tRNA            complement(26880..26967)
                     /locus_tag="ACHKFK_001411"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26931..26933),aa:Ser,seq:gga)
     repeat_region   27311..27521
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=27311..27341
                     /rpt_unit_seq="gttcactgccgtacaggcagcttagaaattt"
     gene            complement(27631..27849)
                     /gene="infA"
                     /locus_tag="ACHKFK_001412"
     CDS             complement(27631..27849)
                     /gene="infA"
                     /locus_tag="ACHKFK_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO9051782.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(28134..28838)
                     /gene="aat"
                     /locus_tag="ACHKFK_001413"
     CDS             complement(28134..28838)
                     /gene="aat"
                     /locus_tag="ACHKFK_001413"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO9051783.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCDEFIRHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(28880..30601)
                     /gene="cydC"
                     /locus_tag="ACHKFK_001414"
     CDS             complement(28880..30601)
                     /gene="cydC"
                     /locus_tag="ACHKFK_001414"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO9051784.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGIYLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMCEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(30602..32368)
                     /gene="cydD"
                     /locus_tag="ACHKFK_001415"
     CDS             complement(30602..32368)
                     /gene="cydD"
                     /locus_tag="ACHKFK_001415"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO9051785.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLFLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPASQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQTALDNAWVSEFLTLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(32491..33456)
                     /gene="trxB"
                     /locus_tag="ACHKFK_001416"
     CDS             complement(32491..33456)
                     /gene="trxB"
                     /locus_tag="ACHKFK_001416"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO9051786.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLTGDSG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            34000..34494
                     /gene="lrp"
                     /locus_tag="ACHKFK_001417"
     CDS             34000..34494
                     /gene="lrp"
                     /locus_tag="ACHKFK_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO9051787.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            34629..38672
                     /gene="ftsK"
                     /locus_tag="ACHKFK_001418"
     CDS             34629..38672
                     /gene="ftsK"
                     /locus_tag="ACHKFK_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO9051788.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPTQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPTRPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREVQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNTEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQYQQPQQPVAQQPQYQQ
                     PQQPVAPQQQYQQPQQPVAQQPQYQQPQQPVAPQPHDTLLHPLLMRNGDSRPLHKPTT
                     PLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELN
                     LAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDN
                     AKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKA
                     QPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSAL
                     GVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMM
                     TVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDS
                     RTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVD
                     GITSDSESEGGVGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARII
                     EQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            38831..39442
                     /gene="lolA"
                     /locus_tag="ACHKFK_001419"
     CDS             38831..39442
                     /gene="lolA"
                     /locus_tag="ACHKFK_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO9051789.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            39453..40796
                     /gene="rarA"
                     /locus_tag="ACHKFK_001420"
                     /gene_synonym="mgsA"
     CDS             39453..40796
                     /gene="rarA"
                     /locus_tag="ACHKFK_001420"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO9051790.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            40887..42179
                     /gene="serS"
                     /locus_tag="ACHKFK_001421"
     CDS             40887..42179
                     /gene="serS"
                     /locus_tag="ACHKFK_001421"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO9051791.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            42418..44862
                     /gene="dmsA"
                     /locus_tag="ACHKFK_001422"
     CDS             42418..44862
                     /gene="dmsA"
                     /locus_tag="ACHKFK_001422"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO9051792.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATKMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQEFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGISNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            44873..45490
                     /gene="dmsB"
                     /locus_tag="ACHKFK_001423"
     CDS             44873..45490
                     /gene="dmsB"
                     /locus_tag="ACHKFK_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO9051793.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            45492..46355
                     /gene="dmsC"
                     /locus_tag="ACHKFK_001424"
     CDS             45492..46355
                     /gene="dmsC"
                     /locus_tag="ACHKFK_001424"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO9051794.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMLGLWGLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGAELANIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPEVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(46391..47017)
                     /gene="ycaC"
                     /locus_tag="ACHKFK_001425"
     CDS             complement(46391..47017)
                     /gene="ycaC"
                     /locus_tag="ACHKFK_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO9051795.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNENFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            47331..48479
                     /gene="ycaD"
                     /locus_tag="ACHKFK_001426"
     CDS             47331..48479
                     /gene="ycaD"
                     /locus_tag="ACHKFK_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051796.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEQMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTALTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            complement(48576..49316)
                     /gene="pflA"
                     /locus_tag="ACHKFK_001427"
     CDS             complement(48576..49316)
                     /gene="pflA"
                     /locus_tag="ACHKFK_001427"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO9051797.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMSEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(49508..51790)
                     /gene="pflB"
                     /locus_tag="ACHKFK_001428"
     CDS             complement(49508..51790)
                     /gene="pflB"
                     /locus_tag="ACHKFK_001428"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9051798.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(51845..52702)
                     /gene="focA"
                     /locus_tag="ACHKFK_001429"
     CDS             complement(51845..52702)
                     /gene="focA"
                     /locus_tag="ACHKFK_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO9051799.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        13120 a        13033 c        14050 g        12613 t
ORIGIN      
        1 gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc acttctggtc
       61 cggcggcttc gaagatgcga tgttcgctgg ctggatggtc taacagcgcc accagatagt
      121 gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg aacccagcgt ggcggcgtta
      181 acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa cctgcaccaa
      241 cgattattcc ggcacgcaat tcggtcaccg ggacacccgc ttcacgaaga atatccgccg
      301 tagcctgacg ggcgcgcaga tggtccgact gctcatgtgg cggggcctgc aacgaactga
      361 gaaagattaa ttgctttacc ggtacttcgc gcagcgcatc gcggacgttg agagccacct
      421 ggcgctcctg agcgataaaa tcaccgcctt ctcccatgct gtgcaccaga aaatagaccg
      481 tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg actttatggc
      541 aactgacatt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc gccgccagga
      601 tctgatgccc ctgctggctg agtgttcgca ccagatgctg accaatgtag ccactggcac
      661 cgagaactaa aatgcgttgc ggcacgtctc cctccttaac gcgccaggaa tgcgcgccag
      721 tgggcggcga cttccgccag ctgagcgcgc gagacgtcaa gatgcgtcac caggcggaca
      781 atcggcgagg cgttaatcag tacgtttctc gctttcatgt attcgcctaa cgcggcagca
      841 ttttcttccc cgacgcgaac aaacagcata ttggtgtcct gacgcatcac atccgcgcct
      901 gcttcacgca gttgcttcgc catccaggcg gcgttgtcgt ggtcttcctg caaacgtgcg
      961 acgttatttt tcagcgcata cagcccggcg gcagccagaa tgccggactg gcgcatcccg
     1021 ccacctgtca ttttccgcca gcgaatggca cgtttaatgt aatcacgatt accgacgagt
     1081 aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga gtcacaatat
     1141 tgtgtgacct ctttcagttc gcagccgtaa gccaccacgg cattaaagat gcgcgcaccg
     1201 tcaacatgca gcgccagatt gcgcttgcgg gtaaattccc atgcctcttt taagtattca
     1261 cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt gcgggcgaaa
     1321 tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt gccgtcggca
     1381 gctgcgtcga tgggttgcgg ctgaatactg cccagcactg ctgcgccacc ggcttcaaac
     1441 agatagttat gcgcggcctg accgacaata tactcttcgc cgcgttcgca gtgactgagc
     1501 agagcgacca ggttagcctg ggtgccggtc ggcagaaaaa tggcggcttc tttaccggaa
     1561 agctctgccg catagtcctg cagagcatta acggtagggt cgtctccgta aacgtcgtcc
     1621 ccaaccgggg cggccatcat tgcttcgagc atggcgcggc tcggtcgggt aacggtatca
     1681 ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc tttttacctt
     1741 agccagttcg ttttcgccag ttcgatcact tcatcaccgc gcccgctgat gattgcgcgc
     1801 agcatataca ggctgaaacc tttggcctgt tcgagtttga tctgcggtgg aatggctaat
     1861 tcttctttgg cgacaaccac atccaccaac accggaccgt cgatggagaa ggcgcgttgc
     1921 agagcttcat cgatttcaga cgctttttct acacgaatac ccttaatgcc gcaggcttcg
     1981 gcaattcggg caaagttggt gtcgtgtaat tcggtgccgt cagtcaaata gccaccagct
     2041 ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat tttcactggc
     2101 agcttcatct gcactaccga gaggaaatcg cccatcaaca tgctaaaacc gccatcgccg
     2161 cacatggcga ccacctggcg ttctggctcg gttgcctgcg cgcccagcgc ctgcggcatg
     2221 gcgttagcca tcgaaccatg gttaaacgaa cctaacaggc gacgcttgcc gttcattttt
     2281 agataacgtg ccgcccacac cgttggcgta ccaacgtcac aggtaaagat ggcgtcatcg
     2341 gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt ctcgctcggt
     2401 ttagctaaat catccagccc tttgcgggcg tcgcggtaat cttccagcgc tttatccaga
     2461 aacttgcgat cggttttttc ttccaccagt ggcagcaatg cccgcagggt tgatttgata
     2521 tcgccgacca gcgccatatc taccttgcta tgcgcgccga tgctggctgg gttgatatca
     2581 atctgaataa ttttggcatc ggtcgggtag aacgcgcggt agggaaactg agtaccgagc
     2641 agcactaacg tatcggcgtt catcatggta tggaaacctg acgaaaagcc gattaatccc
     2701 gtcattccaa catcatatgg attatcgtat tcgacatgtt ctttaccgcg cagggcatga
     2761 actataggcg ctttaatttt cccggcaaac tcaactaact ctttatgcgc ccccgcacag
     2821 ccgctgccac acatcagggc gatattgctg gaataacgca gcagttgcgc cagtttgcgt
     2881 aactcttctt cttccggtgt tactaccggc tgtggcgcat gataccagtg ggtagttgcc
     2941 ccttctggcg caggttttaa cgccacatcg cccggtaaca caacaacgga aacgccacgg
     3001 ttaagcaccg ctttgcgcat agcaattgcc agcacttgtg ggatctgctc tgggctggaa
     3061 accagctcgc aatagtgact acattcgcgg aatagctctt gtgggtgggt ttcctggaaa
     3121 tagccgctgc caatttcgct ggagggaata tgagcggcaa tcgccagtac cggaacgtga
     3181 ttgcggtggc aatcgaacag gccgttgatt aagtgcaggt tgccgggacc gcacgatccg
     3241 gcacagaccg ccagttctcc gctaagttgt gcttcagcgc cagcggcaaa cgccgccact
     3301 tcttcatgcc gggtggacat ccactcgatg gtgcccatgc gattaagact gtcactaaga
     3361 ccgttcagag agtcgcccgt gactccccag atgcgtttca cgcctgccga ttcgagtgtt
     3421 ttggcgatat aagctgcaac cgtttgtttc atggttctcc atctcctgaa tgtgatgacg
     3481 gtaacaagtt tagttcatct gacggagggg gaagggatgg gagaaaaagg aggcactaac
     3541 ggttaaatag cccgatgaaa ggaatatcat cgggcatcag gcgattatgc gagaaccaaa
     3601 tccccctgcg gatggcagga gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc
     3661 agcgtcattg tgctgctcac cgtatattca ccggaaatca ctttcgtctt acagcagccg
     3721 caaacacccg cacgacaggc ggcgacaacc ggaacgttat tgctttccag cgcctccagt
     3781 agcgtggtgc caaccggagc gtaaaattct cgtgccggtt gcagtttggt gaatttcaga
     3841 ccgctggtcg ccgcttccgc tactggggtg aagaatttct ctttaaagaa acgcgtcacg
     3901 ccgagcgctt tcacttcctg ctctacccaa tccatatatg gcgcagggcc acaggtcatc
     3961 acggtacgtg aggctaaatc agggacacct gccagcagtt cgcgagtgag acgaccagcg
     4021 ataaagcctt cggtaacgtt attttccgcc accagcgtta ccggatagtt acgccactca
     4081 tcggcgaaaa tcacatcctg cggcgtacgc acgttgtaga tcacctgcac atcggcctgt
     4141 ggacggttct tcgccagcca gcgacgcatc gacataatcg gcgtaacgcc gcagcctgcc
     4201 gccagcaaca ggaatttatc ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc
     4261 gaaagccaga gataatcacc gcgttttaca tcgcgcgtca gccaccggga gccgacaccg
     4321 tcatcaatcc gccgcacggt cagggtgatg tattcactca cgcctggcgt ggaggaaatg
     4381 gtgtaagcac gcagcgtttc cgctgagtta cgcacgctga ccagcgcata ttgcccggcg
     4441 cgatacgggt agtaatcgtg gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc
     4501 gtaatgtgat gaacctgcat ccgccacggg cattgattcg ttggcatcgt catcgacaaa
     4561 ctccttacgc gctcaacagt tgcttcatgt cttcttcaac agtggtgata gaacgcaggc
     4621 cgaatttctc gttcagcacc gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc
     4681 cggtgacgat atttttcaca cccagagaaa gcagcgtcag cagaatgaca atcgctttct
     4741 gttcaaacca ggagagcacc agcgacagcg gcagatcgtt gactccgcag cccagtttct
     4801 ctgccagcgt taccgccaga ataattgctg agtaagcatc gttacattga ccagcatcta
     4861 ccagacgcgg cagaccttcg atatcgccaa actcaagttt attaaagcga tatttaccgc
     4921 aagcgagggt caggatcagg cagtcatccg gcacgctggt ggcgaaatcg gtgaaatagt
     4981 gacgctcgcc gcgtgcgccg tcacagccgc caagcaggaa gatatgacgc agtttttcac
     5041 ggctcaccag atcaatcagc gtatcggctg cgccaagcag tgtctggcga ccaaagccca
     5101 cggtgatcag gtgcggaatt tcgctgtacg ggaagcccgc catctgttgc gcctgggcaa
     5161 tgactgcaga gaaatcttcg ccatccagat gacgcacacc cggccagcca acgatgctgc
     5221 gggtccagat acgatcgtca taagcgccta cggttgggtc gatgatgcag ttcgaggtca
     5281 tcacgatggg gcctgggaaa cgagcgaact ccacttgctg attctgccag ccgctgccgt
     5341 agttaccgac cagatgcttg aatttacgca gctccgggta accatgcgca ggcagcattt
     5401 cgccgtgggt gtagacatta acgcccgtgc cttcggtctg ttccagcagg ttgtagagat
     5461 ctttgagatc gtgaccggaa atcagaatgc atttacccgc cgtcgctttg acattgacct
     5521 gggttggggt cgggtgaccg tatttaccgg tttcgcctgc atccagaatg ctcatcactt
     5581 tgaaattcat ctggccgatt tccattgaac actcaagaag cgcgttcata tcggcaggcc
     5641 aggtccccag ccacgccatg attttatggt actgggcata aatatcgttg tcgtattgac
     5701 cgagaacatg cgcgtgttcc atataggccg ccgcaccttt caggccatac aggcacagca
     5761 gacgcaggcc gagaatgttt tcgccaatcg ccgctttatc tttgttaggg gtaaattctg
     5821 ccgcctgacg ttgcagctcg ccgagatcgt cgctcaccag ttgcaggtca gccatcgggt
     5881 tatcgacgcg ggcgttggca tctacagcca ggcattgtgc tttcagcgcc tcgcgcaggg
     5941 caatcgcttc acgagcgtag ccgacaatac gcggagaatc gaagttaacg ttggtcaggg
     6001 ttgagaaaaa ggcacgtggc gcaaagctgt ctacatcgtg gttgatgatg ccgtattcac
     6061 gtgctttcac tgcccaggcc gaaagacctt gcagcgccgc gatgagtaaa tcctgaaggt
     6121 cagaggtttc cgccgtttta ccacacatcc cctgcgcgta tgagcagccg tttcctgccg
     6181 gagtacggat agtttgttca cattgcacac aaaacatgat cacacctttt aaagttatat
     6241 ttaatataca tgtttaaggt taagacgctt aacgcgggga taaaagagat ttttaatgca
     6301 actttaaggg agattgattt agcgcaattt tggcggcaga gatctaccgc cagagaggta
     6361 ttatgcagag aaaaaggcga tgagaatcgg cactaacaga ctaagaataa aaccgtgaac
     6421 aatcgccgcc gggaccatat ccagcccgcc tgtacgttga agaacgggca gggtgaaatc
     6481 catcgacgtc gcaccgcata agcccagggc agtagagcgg ctacggcgaa tcagcccagg
     6541 gattaacata atagcaatca gctcacgggc cagatcatta aaaaacgccg cgctcccgat
     6601 taccggacca aaagattcgg tcaataaaat accggaaaga gaataccagc cgaaaccgga
     6661 ggccattgcc agtgcggtat tgatgggaag atcaagaata aaggcgttaa ttaaaccacc
     6721 aattaatgaa ctggcaacca ccaccacggc gacaatcatt ccccggcgat taaggacaat
     6781 ctgctttaag gtcatgccat tattgcgcaa ctgaataccg acgaggaaaa gtagcagaat
     6841 taaggtgtat tcactggctt cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat
     6901 ggcaaaacca atcactacta cgccgcacag ttttagcgac tccagcgcca tcgcaatacg
     6961 cgacgggagt ttttcttgat gatggtggtt gcgccatggc aggccgcgct ccagccacat
     7021 cagggcggca atattacaca gtaaaataac ggtaatactg acggcagaat aatgcagaat
     7081 tgccaacaag ttactggcga ggttatcgag aaacgccaga ctgataccca taaaaaagag
     7141 aataaggtaa accatccaac ttaatagctg attaataact cttaatgcag ctttttggcg
     7201 aagcggaatg aggtaaccca caatcagggg aaccagaatg attaacagcc cagaaaacat
     7261 gaaaaaccag tccttgcaaa gaagattcga aatgcgcgat gacacactac tgaaagcgga
     7321 aggacgagta aagttgcaat taaaaggaaa tgttatgcat cagaggggga tttttaattc
     7381 tatatgagtg tttagcattg ttgcgctcct ttcatgtgca atgccatata aagccactgg
     7441 cgcagggagc ggccagttgc cgccgctccc tgcccccccg gccttcgggg aacgcctcgg
     7501 cgattttaac gccaccaaca cccgtgcggt ttgtaacgtc gaagataaaa ccaccagggc
     7561 agacaacatg ccaatgctaa ttaatcgcgt ttttccagta gagtccggta aatcagacca
     7621 ccgataatac cgccgacaat tggcaccacc cagaagaacc acagttgttc taatgcccag
     7681 ccgccctgga agatagcaac cgcagtactg cgcgccgggt taacggaagt gttagtcacc
     7741 ggaatactga ttaagtgaat gagggttaag gccagaccaa tggcaattgg cgcaaaacca
     7801 gcaggagcga atttgtcagt tgcgccgtgg atcaccaaca ggaaacctgc actcaatacc
     7861 agttcaacta ccagcgcgga aagcatggaa taaccgcctg gtgaatgctc gccataaccg
     7921 ttagaagcaa aaccgctggc tgccgcgtca aaacccgttt taccactggc aattaaatac
     7981 agcagcgccg ccgcaacaat accgccgaca acctgggcaa ttacatagcc aacgacttct
     8041 tttgccggga aacgtccgcc agcccataaa ccaatagtga ccgccgggtt aaaatgacca
     8101 ccagaaatat gaccaacagc aaaggccatt gtcagaacgg tcagaccgaa cgccaacgcc
     8161 acgccggcaa aaccaatgcc taattccggg aagcttgcgg ccagtacggc actaccacag
     8221 ccgccaaaaa caagccagaa agtaccaaaa cattcagctg ctaattttct gaacatatcc
     8281 acctcaatta aaaattgacc ctgtgaaaaa tatggtcgtt ttatagggtc gtcgtaaaaa
     8341 agtgacgacg gaaataatgc gcggctattt taaaaacgaa ggcaagtcat tcaccagata
     8401 aataaatcca gtaaatttga tttagggcaa cagcgggttg ccccatatag tcatttgtct
     8461 gattgacagt gtagtgcaag cgaaagattt aatcctttag gcgtaataaa aaataattta
     8521 tcatgctaat tatttgattt tgttgttttt gcagacttgt cagcaagagg gagtataacg
     8581 cgattattcg ctcatttttc agacatttgc catgcttaaa tgtgatgtca tcacgtatta
     8641 gcatggcctt tcccgttata ctgccagcgt aaaggataag tcacatattt ctggagggga
     8701 tatgattctt gagcgcgttg aaattgtggg ttttcgcggt atcaaccgtt tgtcgttgat
     8761 gctggaacaa aacaacatcc tgattgggga gaacgcgtgg ggtaaatcca gcttgctgga
     8821 cgccttaacc ctgctgctat cgccagaatc agaactctac cattttgagc gcgacgattt
     8881 ctggttcccg ccgggagata tcaacgggag agaacatcat ctgcatatta ttttgacctt
     8941 ccgcgagtcg ctgccaggcc gacatcgggt tcgccgctat cggccgctgg aagcgtgctg
     9001 gacgccatgc accgatggtt atcaccgtat tttttatcgt ctggaagggg agagtgcgga
     9061 agacggcagc gtgatgacac tgcgcagttt tctcgataaa gacggacatc cgattgatgt
     9121 tgaggatatt aacgatcagg ctcgtcatct ggtgcgttta atgccggtgc tgcgcttgcg
     9181 tgatgcccgt tttatgcgcc gtattcgtaa cggcacggtg ccaaatgtcc ccaatgtgga
     9241 agtcaccgcg cgccagctcg atttcctcgc ccgtgaatta tcctcacatc cgcaaaatct
     9301 ctctgatggg cagattcgtc agggactttc cgcaatggta cagctgcttg agcattattt
     9361 ttctgagcag ggagctggac aggcgcgata tcgtttaatg cggcggcgag ccagcaatga
     9421 gcaacgaagc tggcgctatc tggatatcat caaccggatg attgaccgac ctggagggcg
     9481 ctcgtatcgg gttattttgc tcggcctgtt tgctacttta ttgcaggcaa aaggcacatt
     9541 gcgactggat aaagacgccc gtccattgtt gctgatcgaa gatccagaaa cccgtttgca
     9601 ccccattatg ctttcagttg cctggcatct gttgaacctt ctgccattgc agcgcatcgc
     9661 tactaccaac tcgggagagt tgctttcgtt aacgccggta gagcatgttt gccgactggt
     9721 acgagagtcc tctcgcgttg ccgcctggcg tctgggaccg agtggcttga gtaccgaaga
     9781 tagccgacgc atctcctttc acattcgttt taaccgtccg tcatcgctgt ttgcccgctg
     9841 ttggctgctg gtggaagggg aaacggaaac ctgggttatc aatgaactgg cgcgtcagtg
     9901 cggacatcat tttgatgccg aagggatcaa ggtcattgag tttgcccagt ccggactaaa
     9961 gccactggtt aaatttgccc gccgaatggg gattgaatgg catgtactgg tcgatggcga
    10021 tgaagcaggg aagaaatatg ccgctacggt acgcagcctg ttgaataacg atcgggaagc
    10081 cgaacgagaa catttaacgg cgttaccggc gctggatatg gaacatttta tgtatcgcca
    10141 gggattttcc gatgtgttcc accgcgtggc gcaaatcccg gaaaatgtac cgatgaatct
    10201 gcgcaaagtt atctcgaaag cgatccatcg ctcttccaaa cccgatcttg ccattgaagt
    10261 ggcaatggag gccggacgtc gtggtgtgga ctccgtaccg acgctgctga aaaaaatgtt
    10321 ctcacgcgtg ctgtggctgg cgcgcggtcg ggccgactaa ctacaaaaca tcgtggccat
    10381 ttgtggctga atagcgtcga gcatctcata gcgccgacgg tattcagccc gttttttact
    10441 ggcgatttcg gcaatctctt ttcgtgctat ctgtgctgga aggcggtaat ggcgttcagc
    10501 atcacatacg ccgccaaccg attcccagaa agcgttgtaa tcggcatgga tcttgccttc
    10561 tttatcgcga taacgcaggc tgcggtaaat atgcgtttca ttgctgacgg caataatctg
    10621 ctctacctgc aaacgttggg caaacagaca ggccgcttcc atcacgaggc gtttgggaaa
    10681 tagcccgtgg caggctttcg tcgcattctg gatttcctgg tgtggaattt cccattttgc
    10741 gccttgcagt ccgccaataa acatcgtcct tttcccctga tattcacaca gggtaaaagt
    10801 gagctctgcc agaggaatac cttcgctgtt gcggaacagg atggtgctgt caccttcttt
    10861 atccattgag atcatcatgg tcagctcaag cgtgaactgc tcgccgtttt tgccttccag
    10921 cttcgccagt tgcagcccgg gggtattcaa atataagctg aattcttccg ccgacataca
    10981 cccgcggagt aacgcataat ggtaacgtaa cgcctccagc aattgcttac ggctaagatt
    11041 cgccgcaagg taagggcgat gtagacgtac aggcagtcgc ggctggcgcg ttaacaacac
    11101 attgagatta ggccagtggg aaagttcgtt catccactca acgcttaaac gcggcataat
    11161 caacgagcgc agcaaaaatt tctggcgaaa actacggcga tgccagaatt tacccggccg
    11221 acactgtcct cgtgccagac taagaaaaag tgacaggctg ctgagagatt cagatgacgt
    11281 aaaggtccgt tcagttagct gcgacatatt catgaaatca atggttatac atgacgtcga
    11341 tttaaccatt gcgtatctta accaaacatc aatagtgtga ttattaacgt aaattttagg
    11401 gttttgttga tatttcgttg aagttaatga cccggattgg catatggagt attcagaata
    11461 tttatgaaaa agcggaaaac cgtgaagaag cgttacgtta ttgcgatggt gatagtcatc
    11521 gccgggctga ttacgttatg gagaattctt aacgcacccg tgccgactta tcagacgctg
    11581 attgtgcgcc ccggtgattt acagcaaagc gtgctggcga ccggaaagct ggacgcgctg
    11641 cgtaaggttg acgtgggcgc gcaggtcagc ggtcagttga aaacgctgtc ggtggcgatt
    11701 ggcgataaag taaaaaaaga ccagctttta ggggttattg atcctgaaca ggctgaaaac
    11761 cagatcaagg aggtggaagc aacgctgatg gagctacgcg cgcagcggca gcaggcggaa
    11821 gcggagctga aactggcgcg ggtgacgtat tcccgtcagc aacgtcttgc acaaacgcaa
    11881 gcggtttcac agcaggatct cgataacgcc gcgacggaga tggctgtgaa acaggcgcaa
    11941 attggcacca ttgacgcgca aatcaagcgc aatcaggcct ctctcgatac ggctaaaacc
    12001 aatctcgatt acacccgcat cgttgccccg atggccgggg aagtcacgca aatcaccact
    12061 ctgcaaggcc agacggtgat tgccgcacaa caagcaccga acattctgac gctggcagat
    12121 atgagcacca tgctggtaaa agcgcaggtt tctgaagcgg atgtaatcca cctgaagccg
    12181 gggcaaaaag cctggtttac ggtgcttggc gatccactga cgcgctacga ggggcaaatc
    12241 aaggatgtgc taccgacgcc ggaaaagatt aacgacgcta ttttctatta cgcccgtttt
    12301 gaagtaccca accccaatgg tttactgcgg ctggatatga ccgcgcaagt gcatattcag
    12361 ctcaccgatg tgaaaaatgt gctgacgatc cctctgtcgg cgttaggcga tccggttggc
    12421 gataatcgtt ataaagtcaa attgttgcgt aatggtgaaa cacgcgagcg tgaagtgacg
    12481 attggcgcac gtaacgatac cgatgttgag attgtcaaag ggctggaagc gggcgatgaa
    12541 gtggtgattg gtgaggccaa accaggagct gcgcaatgac gcccttgctc gaattaaagg
    12601 atattcgtcg cagctatcct gccggtgatg agcaggttga ggtgctgaag ggcatcagcc
    12661 tcgatattta tgccggtgag atggtggcga ttgtcggcgc ttcgggttcc ggtaaatcga
    12721 ccctgatgaa tattctcggc tgcctggata aggccaccag cggcacctat cgcgtcgccg
    12781 gtcaggatgt tgccacgctg gacgccgatg cgctggcgca actgcgccgc gagcatttcg
    12841 gctttatttt ccagcgttac catttgcttt cgcatttaac cgccgagcaa aacgttgaag
    12901 tacccgccgt ctatgccggt cttgagcgga aacagcggct gcttcgcgcc caggagttac
    12961 tgcaacgact ggggctggaa gaccgtacag agtattaccc ggcacagctt tcgggtggcc
    13021 agcaacagcg cgtcagcatc gcgcgggcat tgatgaacgg cggtcaggta attcttgccg
    13081 atgaaccgac cggcgcactg gacagccatt ctggcgaaga ggtgatggcg atcttgcatc
    13141 agctgcgcga tcgcgggcat acggtgatta tcgtcaccca cgatccgcag gttgctgctc
    13201 aggccgagcg ggtgatcgaa attcgcgacg gcgaaattgt gcacaatcct cccgccgttg
    13261 aaaaagtgaa tgctactggc gggacggaac ctgttgtcaa cacggcgtct ggctggcggc
    13321 agtttgtcag cggttttaac gaggcgctga cgatggcatg gcgggcgctg gcagcgaata
    13381 aaatgcgtac tttactgacc atgctgggga ttattatcgg tattgcgtcg gtggtttcca
    13441 ttgtcgtggt gggcgacgcc gccaaacaaa tggtgctggc ggatattcgt tctattggta
    13501 cgaatactat tgatgtctat cccgggaaag attttggcga tgacgatccg caatatcagc
    13561 aggcgctgaa gtacgacgac ttatcgctat ccaaaaacaa ccgtgggttg cctctgccac
    13621 gcccgccgtc tcgcaaaacc tgcgcctgcg ttataacaat gttgatgtgg ctgccagtgc
    13681 caatggcgtg agcggcgatt attttaatgt ctatggcatg accttcagtg aagggaacac
    13741 ctttaatcag gagcagctga acggtcgtgc gcaggtcgtg gttctcgaca gtaatactcg
    13801 ccgccagctt ttcccccata aagcagatgt ggttggcgag gtgattctgg tcggcaatat
    13861 gcccgccaga gtcattggtg tggcggaaga aaaacagtcg atgtttggta gcagtaaagt
    13921 gctgcgtgtc tggctgccat acagcacgat gtccgggcga gttatggggc agtcgtggct
    13981 gaactccatt actgtcaggg tgaaagaagg ctttgacagc gctgaggcgg aacagcaact
    14041 cacgcgttta ctttcactgc gccacggtaa gaaggatttc tttacctgga acatggatgg
    14101 cgtcttgaaa accgttgaaa agaccacacg tactttacaa ctgtttctga cgctggttgc
    14161 ggtgatttcg ctggtggtgg gcggtattgg tgtaatgaat attatgctgg tgtcagtgac
    14221 cgagcggacg cgggaaattg gcattcgcat ggctgtaggt gcgcgagcaa gcgatgtttt
    14281 gcaacagttt ttgatcgaag ccgtactggt ttgcctggtt ggtggcgcgt tgggaataac
    14341 actgtcactg ttaattgctt tcaccttgca gcttttctta cccggctggg agattggttt
    14401 ttcaccgtta gcgctgctgc tggcgtttct ctgctcgacg gtcaccggga ttttatttgg
    14461 ctggttaccc gcacgaaatg cggcacgact ggatccagta gatgctctgg cacgagagta
    14521 atttttgaga taaaaatgcc agccgatcgg gctggcattt tgcctttagg atgtacacaa
    14581 tgagacagaa gagctatgcg actgccgctt ctacttcgac gggcacaata acactggcgt
    14641 gattgccttt tggcccctgg tggacatcaa actgaacgga ttgtccagct tttagcgttc
    14701 tgtaaccatc catctgaatg gtggaataat gagcgaaaat atcttcgccg ccgccttcag
    14761 ggcagatgaa accaaaccct ttggcattgt tgaaccactt aacagtaccc ttttccatgc
    14821 ttcgacatcc ttcgcaaatc ttatacaagt aagatggaat aaaccggggt cagagagggg
    14881 gctgttcaaa acctcgccaa ctctagaaat acaatttaga gaattagggc gagccgtcaa
    14941 gcatttgaca ggggccaggg ggcaggtatg aatcaaaaat ttgaagcagt taacgctatt
    15001 gacaggaatg tgacagatgt cgctgatgcc aacgatagat gatagttatc tatcatgtgg
    15061 agtagattgg tcaggcaaat aagctcttgt cagcggcagg gcgttctgcc gataaccgta
    15121 accgaagatg ataactgaca atgggtaaaa cgaacgactg gctggacttt gatcaactgg
    15181 cggaagaaaa agttcgcgac gcgctaaaac cgccatctat gtataaagtg atattagtca
    15241 atgatgatta tactccgatg gagtttgtta ttgacgtgtt acaaaaattc ttttcttatg
    15301 atgtagaacg tgcaacgcaa ttgatgctcg ctgttcacta ccaggggaag gccatttgcg
    15361 gagtctttac cgccgaggtt gcagaaacca aagtggcgat ggtgaacaag tacgcgaggg
    15421 agaatgagca tccattgctg tgtacgctag aaaaagcctg aatgcaggca taaaaattgg
    15481 gggaggtgcc tatgctcaat caagaactgg aactcagttt aaatatggct ttcgccagag
    15541 cgcgcgagca ccgtcatgag tttatgaccg tcgagcactt gttactggcg ctgctcagta
    15601 acccatctgc ccgggaggcg ctggaagcgt gctctgtgga tttggttgcg ctccgtcagg
    15661 aactggaagc ctttattgaa caaaccacac ccgttctgcc tgccagtgaa gaggagcgcg
    15721 acacacagcc gacgctgagt tttcagcgcg tactgcaacg tgcggtcttc catgtccagt
    15781 cctccggtcg caatgaggtt accggtgcaa acgttctggt cgctatcttt agcgaacagg
    15841 agtcgcaggc ggcatatctg ttgcgcaaac atgaagtcag ccgtctcgat gtggtgaact
    15901 ttatctctca tggcacgcgt aaagacgagc cgacacagtc ttctgatcct ggcagccagc
    15961 caaacagcga agaacaagct ggtggggagg aacgtatgga gaatttcacg acgaacctga
    16021 atcagcttgc gcgagtgggc ggaatcgacc cactgattgg tcgtgagaag gagctggagc
    16081 gtgctattca ggttctctgc cgtcgccgta aaaacaaccc gctgctggta ggggagtctg
    16141 gtgtcggtaa aaccgcgatt gcggaggggc ttgcatggcg aattgttcag ggcgatgtgc
    16201 cggaagtgat ggctgactgt acgatttact ctctcgatat cggttctctg ttagcgggca
    16261 caaaatatcg cggcgacttt gaaaaacgtt ttaaagcgtt gctcaagcag ctggagcaag
    16321 acactaacag catcctgttt attgatgaga tccacaccat tatcggtgcg ggtgcagcgt
    16381 ctggtggcca ggtcgatgcg gctaacctga tcaaaccgtt gctctccagc ggtaaaatcc
    16441 gcgtaattgg ttcgacaacc tatcaggagt tcagcaacat ttttgagaaa gaccgtgctc
    16501 tggcgcgtcg cttccagaaa attgatatta ctgaaccgtc aatcgaagaa actgttcaaa
    16561 tcatcaatgg cctgaaaccg aagtatgaag cgcaccacga cgtgcgttat accgcaaaag
    16621 cggtgcgtgc ggcggtagag ctggcggtga aatacattaa cgatcgtcat ctgccggata
    16681 aagcgattga tgttatcgac gaagcgggcg ctcgcgcacg tctgatgccg gtaagcaaac
    16741 gcaagaaaac cgttaatgtg gcggatattg aatccgtggt ggcccgtatt gcacgcattc
    16801 cagagaagag tgtttctcag agtgaccgcg ataccctgaa aaacctcggc gatcgcctga
    16861 aaatgctggt cttcggtcag gataaagcca ttgaggcgct gactgaagcc attaagatgg
    16921 cgcgtgcagg tttaggtcac gaacataaac cggtcggttc gttcctgttt gccggcccta
    16981 ctggggtcgg gaaaacagag gtgacggtac agctttcgaa agcgttgggc attgagcttc
    17041 tgcgctttga tatgtctgag tatatggaac gccataccgt cagccgtctg attggtgcgc
    17101 ctccgggata cgttggtttt gatcagggcg gtttgctgac tgatgcggtc atcaagcatc
    17161 cacatgcggt gctgctgctg gacgaaatcg agaaagcgca cccggacgtg ttcaacatcc
    17221 tcttgcaggt gatggataac ggtacgctga ccgataacaa cggacgcaaa gcggacttcc
    17281 gtaacgtggt gctggtgatg accactaacg cgggggtacg tgaaaccgag cgcaaatcca
    17341 ttggtcttat ccaccaggat aacagcaccg atgcgatgga agagatcaag aagatcttta
    17401 caccggaatt ccgtaaccgt ctcgacaaca ttatctggtt cgatcatctg tcaaccgacg
    17461 tgatccatca ggtggtggat aaattcatcg tcgagttgca ggttcagttg gatcagaaag
    17521 gtgtttctct ggaagtgagc caggaagcgc gtaactggct ggccgagaaa ggttacgacc
    17581 gggcaatggg cgcacgtccg atggcgcgtg tcatccagga caacctgaaa aaaccgctcg
    17641 ccaacgaact gctgtttggt tcgctggtgg acggcggtca ggtcaccgtc gcgctggata
    17701 aagagaaaaa tgagctgact tacggattcc agagtgcaca aaagcacaag gcggaagcag
    17761 cgcattaatc tgattgtctg gtaggttggt gaagtccgtg atctcgtcag gggttacgga
    17821 ctttttattt atggggggag gaggttcaga cccttttttt aatgatgatg ctaagttatt
    17881 gataattagt gctgcgggta ggtaaggata aaaaagggtg gcagcaggag attgagatgg
    17941 ttttgcttta ttaacaacgg gctaaacgtg tagtatttga gttcactgcc gtacaggcag
    18001 cttagaaaag ccggggcgca tccagccgca cgtaaatgct gttcactgcc gtacaggcag
    18061 cttagaaatt atggctcgcc gcgaagaata cgccgccgca gttcactgcc gtacaggcag
    18121 cttagaaatg ttaacggcgt ggctatgccg gataatttac gttcactgcc gtacaggcag
    18181 cttagaaact gaacgttgaa gagtgcgacc gtctctcctt gttcactgcc gtacaggcag
    18241 tattcacact acgatagtgt tagacgtttg gtcgtgcaat gacactctca acttcaaacc
    18301 attagcgtta gcacgcaata acaatcgtaa taattgcgat ggaaatcaat tttcagcaca
    18361 taaatcaatg ctgtactaag cccaatacct tcaaatataa aataatcaca ggatgtgttt
    18421 atgtcttcga attaccttac gccttccgat ctcaaaacca ttctccactc caaacgcgcc
    18481 aatatttatt atctggaaaa atgccgggta caggtgaatg gtgggcgggt ggagtatgtt
    18541 accagcgaag gtaaagagtc gtactactgg aatatcccca ttgcgaatac cacggcgttg
    18601 atgctgggaa tgggaacttc cgttacccag gcggcgatgc gtgaatttgc tcatgccggg
    18661 gtgatggtag gcttttgtgg tacggatggc acgccgctgt attcagcaaa tgaagtggat
    18721 gctgatgtct cctggctcag cccacaaagt gaatatcgcc ctacagagta tctccagcag
    18781 tgggtttctt tctggtttgt tgaagacaaa cgacttgctg ccgcgaaacg ttttcagtta
    18841 atccgcctga cacatatcga taagcactgg cccagctcga aaatgctgcg tgaacacgca
    18901 tttcaacctg atgttaatgc gctgcatacc ctcctgaatc gtactcgtga agaaatagac
    18961 gcggcagaaa accatacgca gcttatgctc gtagaggcca aattgactaa agcgttatac
    19021 aaaatggtca gtcaaaccgt ggggtatggc gatttcactc gcgcaaaacg gggcggtggt
    19081 atcgatatgg ccaaccgctt cctcgatcaa gggaattatc tggcttatgg actggcagca
    19141 gtggcggcgt gggtaacagg tatcccgcac ggtttagctg taatgcatgg taagacacgt
    19201 cgcggcgggc ttgtttttga ccttgccgat ctgattaaag atgctcttgt tatgccgcaa
    19261 gcatttattg ccgcaatggc gggagaagat gctcaggagt ttcgtcaacg ctgtattaat
    19321 atctttcaac aagcggatgc gctggatgtc atgatcacct ctctgcagga aacggcccag
    19381 gcactggcta aggctgacca atgaatgtcc taattatctc acgctgtacg aaaaatgctc
    19441 gcgtggaaag ctgccgtatt attgatcagt ttgccgaacg cactggtgac gcagcatggc
    19501 aaacggtgat tactctggaa ggcgttaata cactccgccg tttactgcgc aaaaccgcca
    19561 ggcgaaatac cgcagtggct tgtcactggc tgaagaagaa tgggcaaacg gagctgatgt
    19621 ggattgtcgg caatattcgt cgctttaatg ctcaaggccg cgttcccaca aatcgaacaa
    19681 cgcaaatcag cctggtgaac aaagaagaaa atcgctggca gtgcgcggaa agtattgcac
    19741 tgctggcggc tatcgctggg ctatttcatg attttggtaa atcaggacgc agctttcagc
    19801 aaagcctgac tcaaaagaat acccgcagtt atcaacctta ccgacatgaa tggatctcac
    19861 tgcggctctt tcaggcgttt gtcggtgatc ggcccgacga tgtgtggctg gcgaagcttg
    19921 gcgaactcac ggcagatgat gaatctgcga ttcttgagcg cctgcaaaaa gacactcctc
    19981 aattttcaaa tagcccgttc agttcgctgc aaccattagc caaaacggta ggctggctga
    20041 tcctttccca tcatcgaatg ccggagaatc tttctccgca gtcggaacaa tcatgtaaag
    20101 ggtgtgaaag ttggctggag aagcaactta atgctgactg gaatgccgtt aaccaccgca
    20161 gagatgactg gtcacaacag gattttgcgc acgtctggca gtttccatca ggaacgccgc
    20221 ttcaaagcgc gagctggcgg gaaaaagcgc gtcagatagc ccgccgggca agcaacgcgc
    20281 taggtttaaa gacatatggg cagatggaac agttgttccc cttacatatg gcgcgttttt
    20341 gtctgatgct tgccgatcat tactattcct caagcgatgc gcagcaaaaa tggcaggacc
    20401 cggattatat cgtctgggca aatagcgatc gccaaagcgg cacgctcaaa caacgggtag
    20461 acgaacacct tgtgggcgtc gcgcatcatg cgtatctgct tgggcggcaa ctgatgcaca
    20521 cacgggcatc gcttccggca attgcccgac ataaaggttt tagccagcgg gccacagatg
    20581 tgaattatcg ctggcaagat aaagcctggg atgtggcgcg agcgttacgt gaacgaagtc
    20641 gtgaagaggg atttttcggc gtcaatatgg cctcgacggg gtgtggtaaa acctttgcga
    20701 acgcccgcat tatgtatgca cttgccgacg agcgagaagg gtgccgcttt accgttgcgc
    20761 tgggattgcg cacgctgact ttacaaaccg gcgatgctct gcgcagtcgg cttgggctgg
    20821 gtgaggatga gctggcggta ctgattggtt cggcggcagt taatcaactg tggcaacagg
    20881 aaaaaataga caatgattgc ggaagtgctt cgcaagagtc gccggcagag gagcaacagt
    20941 tcgttaagta cgaaggcagc ctgcataccg ggacgctgga aaaatggttg aaggatgaca
    21001 gcaaacttaa ggagttagta agcgcgccga ttctggtctc gacgattgat catcttattt
    21061 ccgcgaccga gggcgtgcga ggcgggaaac agcttccggc gatgttgcgc ctgttaacca
    21121 gcgatttagt gctcgacgaa cctgatgact ttgatattgc cgatcttcat gtggtatgtc
    21181 gattgatgaa ctgggccggg atgctgggaa cgcgggtatt actttcttcc gcgacgttac
    21241 cgctaggttt gattcaggcg ctgttcgcgg cttatcttgc tgggcgaaaa atgtggcagg
    21301 cgtcttgtgg tatcaacggc aggcccgtca atatatgctg cgcctggttt gatgaaaaag
    21361 atgctgatgc cacgcaaatt tacgacggac ctgggtttcg tgatgctcat gccaaatttg
    21421 ttgcacgacg agcggtaatg ctggctgaaa aagaacgatt gcatttcggg cgtgtagctt
    21481 cggtttcatc agcatcaggc gcgatacaag atgttaccga aagtgtggcc cagacggtgc
    21541 atacgcaaat gctgaagttg catcaggcgc atcgccagcg acacgaaagc ggtaaaaccg
    21601 tttcgctggg cctggtgcgc ttcgcgaata tcaatccact ggttgcggtg accaaagccc
    21661 tgattgtcat tccttcacca gaagatgtct gcattcatta ctgtgtttac cactcccgac
    21721 acccgctggc ggtgcgttca gatattgaga agcgactgga tcgcgctttt actcgtcata
    21781 acgaactcga tttctggaac aacgaagata tcgcagatgc cttacataac cgtccggaat
    21841 cgcaccatct gtttgtggtg ttggggacat cggttataga agtggggcgt gactgggatg
    21901 cggactgggg aattatcgaa ccaagctcca tgcgttcgat aattcaattt gccggacgta
    21961 ttcagcgcca ccgtagaaat gtgccgacca gtgagaatct ggttattttg cgtagcaata
    22021 tcaaatcatt gcagaaaaaa gcccccgcat tttgtaagcc tggtttcgaa acaaaagaac
    22081 acttgctgaa tagccacgat atttgcgaac tgattccgat cgcggactac cggacgatta
    22141 acgccttgcc gcggatcctg tctgccaata atgccaacaa actggcggag ctagaacata
    22201 gtcgtttatc cgccgaattg atgagcagta aaagcaaaca tgtggtggcg gcgcaatggt
    22261 ggcgtgcacc gctaagctgg aacggctgga tgcagcgcca gacgccattt cgttattccc
    22321 ctttgccgga ggcggtgttt tttctgcata tggatgatga agaaagcgaa gcgcgtttct
    22381 attcccgcac gggcgacaac gagcgtaaag cgcaagggaa tttcaggcga gaagaggtga
    22441 gatgcgccag tggcgtcgtt tgctggggag tgatggatta ccaacacgtt ttgttggatt
    22501 tggctgaaaa gcagagttgt gagatagcaa gcgttgggga aaagtttgcg gaagtgagag
    22561 tacccgtcag tgaagaagat gcggtcgatg actggcgtta tcacccatgg cttggggtgt
    22621 ttcgcgcccc gtgaaggggc gcgggctacc tgtacggcag tttacaagtc cgtatccgga
    22681 gatatctcta tttctaagct gctcaaggca gtttgggtat gttagcgctt caactattgc
    22741 gtggcaaata ttaagttgct ttaatatcac cgatggtgat tacatgagat ttaataatat
    22801 cttttagaag atatctctag gagagtttgt ttgtgtgatc accactggtg ttgtcccttt
    22861 tgctgtgtat gtcagttgta ctgaagccat gttgggctac ggagattttt cagtgaaatc
    22921 agggagcaac ttcgacttga tggattaacg aaaaggagcc gatatggaaa aaacggcgct
    22981 aatacaattt attactgact acatcgcttc gcgaaggcaa cccaaaatag atgcctttga
    23041 aaaagaagca gcaaagcggt tggaacaggg cgaggacgcc agcgttattg ctctggagcg
    23101 gcaggaactt gaagcacgct acctgccacg taactggtta acggatgcgg cgaaacgtgc
    23161 cgggcaaatc aaattagtca cccatggcgc aaaattcagt catggtgatt ctaaggccag
    23221 cagcttttat ctggaaacct ccgctaccga aagctattta aacacagcat cgttggcgaa
    23281 tgtcgctact gacgcgattg gcaacgccgc cgcactggac gtcgcaaaac tgctacaaac
    23341 cgatgtgaac ggagattcac tgcttgccag cctgaagcgt ggtgattatc aggcattaag
    23401 tacgtttgca gaggataaag tgcaactgga gctgtgggta gctgggttta gccaggcatg
    23461 gacaactgga cggccttcca gccataaact ggcgaaacaa atctatttcc ctgttgcgga
    23521 tggttatcac ctgttatgcc cactgttttc cagttcactg gcccaggcga tgtatgaaaa
    23581 gctaaccgcc gggcgcttta gtgaggaatc gaaagccatt cgcgatgccc gcaaagcagg
    23641 aaaatggcac tcccagccag atatccggtt tcctaatctt gccgaaatgc atttcggcgg
    23701 cacaaaaccg cagaatattt ctttgctaaa cagtgtgcgt ggtgggcgcg tctggctgtt
    23761 gccgtcaatg ccgcctgcct gggggccgct ggatcgcgca ccacaaaaaa tgcacagtat
    23821 ttttgcctta cgcggcgatt tcaatagggc ggcttcaggc atcatagccc ggatgactta
    23881 cctgctgaag gttgacacaa ataatgttca tatccgaact gtgcgggcga aatatattga
    23941 tgagttgatc gatttgctgt tcatgcaggc atccgctttt cagcaagaaa aatggcaggg
    24001 atggtccgca caatcaccag acttacctcg ccaccaacaa ctttggctgg acccgtggcg
    24061 aagcctctca gatgagactt tcaaacagga gcgtgagaaa aacgactggc aagtaactgt
    24121 cgccgatgat ttcgcacgct ggctgaacta tcgcctgaaa aaatccagtt ttgacgtagg
    24181 cgctgtcgaa caaaaagagt ggcgcagcca gtctctgttc gctcaacgga tgcgtgaaat
    24241 ggaagctgtt ttgcaggagg cgctgaaatg agttatctgc tcttgttacc ccatatacgc
    24301 atcgaaaacg ctaacgctgt ctccgggttg acctggggat tcccgtcgat gacccatttt
    24361 ctcggttatg tccatgcgct ttctcgcaaa gtcgtggatg aatttggcgt aagttttgat
    24421 ggttgtgcgg tagtaagcca tgaacagcat attcaggcgt acagctctgg gcgcgatttt
    24481 cagttcgctc tcacccgtaa cccattaacc agagaaggta aaaccgcctc gttcaatgaa
    24541 gagggccgca tgcacttgac ggtttctcta ctgattgaat gtaatggcga aatcaccaat
    24601 ggtgaatatg gcagaaaagt tctttgtgac tatctgaaaa tgctttgcca gagccacaaa
    24661 ctggcgggcg gcagcattgt cagtatgcgc gatccgcagc tttttcatgc gcctgaagat
    24721 gaaaagcaac tgcgtaaaat cgtctggcgg ttaatgccgg gttatgcgct ttacgatcgc
    24781 agtgaatggc tggctgaaca tcaccaacaa catcccgaca tctcgttgct ggatgcctgg
    24841 cttgatttcg ccgcgattaa atatcaggcc gaatctccgg ctgaagacaa cagcgcaaaa
    24901 tgggtatacc agccgaagcc aatgcctggt ttcctggtac cgttaatgtg tggctaccaa
    24961 cgcatttctc ctgtttacgc acccggtgaa gttgaaaatg cgcgcgacac cgtgacgcca
    25021 tttgcctttg ccgaagcggt atatggcatt ggcgaatggc gcggattaca tcgcactaca
    25081 gacttgcaag cgctgatgtg gcgttaccgc acaacggaca ctggctacta ttgttcggca
    25141 acgccagttg ttgatgattt tacctttaat gaatatgacg atttagaata aggacataat
    25201 tatggcaaag gctcctgtag caataaaaac ggcgtcggta ctggcatttg agcgcaaact
    25261 ggcaacctct gacgcggtaa tgtacgcagg taactggcaa ggaaatgaat ggcaacccat
    25321 tgagattcag gagaaagcgg tccgcggaac catctctaat cgcctgaaaa atgccattgt
    25381 cagcgatccc acaaaactgg atgctgaaat ccaaaaagcc aacctgcaac gggtgaatgt
    25441 ggcttcgctt ccggtagata cggatacgtt aaaggtggtg tttacgctgc gtgtgctggg
    25501 taatttatcc acaccgtcag tatgtaatga tatggcttac caggaggcgc tttccggggt
    25561 cattgaaggt tacatcagcg aacatggctt taaagagctg acgcttcgct atgcgctgaa
    25621 tctggcaaat ggtcgttttc tgtggcgcaa ccgcattggt gccgaacaga tccaggtgaa
    25681 agtgactgct aacgagactt catggacttt taacagccat gacttctctt tacgtcaatt
    25741 tgaccaggga caggcagcgg tcacgcagct ggcggccatt atagagcaag gattaagcgc
    25801 aaaagagtgg gtgatgctta ccgttgaagc gcaggttcgt cttggtgccg ggcaagaagt
    25861 ttttccgtcg caggagctgg tactcgacag caacagtagt aaaagtcgcg tgttatacca
    25921 ggtggcaggc attgccggta ttcactctca gaagattggc aatgctttac gtaccattga
    25981 tacctggcat ccaaaggttg atgaattggg ggctatagcc gtggaacctt acggttctgt
    26041 aaccagtcgc ggtgtggcct gccgtcaacc gaaagaaaaa ctcgactttt ataccttact
    26101 ggacaattgg gtgactaaag ggatgaaacc agacgttaag cagcagcact acgtgatggc
    26161 tgtgctgatc cgtggcggtg tctttggtga gaaatcggaa taaggagctg ccaatggatc
    26221 actaccttga gatccgcgtt ctgccagatc ctgaattttc gagcgaaatg ttgatggcgg
    26281 cgttatttgc aaaactgcat cgggtattag gtgcaagagg gcaaggcgat atcggcgtga
    26341 gtttcccgga cgtaaatgtt atgcctggaa cgcatttacg cttgcatggt agcgcccagg
    26401 cattgcaaga acttgaagcc tcgacgtggc gtaaagggct gacagattac tgccaatgtt
    26461 cgcctgtcac gccagttcct gaaattaaag gctggcgagt cgtaagtcgt gtacaggtaa
    26521 aaagtaaccc tcagcgtctg ttaaggcgtt cagtgaaaaa aggctggcta actgaagaac
    26581 aggcgattga acgactggcg acgcaggctg aacagcggac cgatttaccc tttcttaata
    26641 tgaagagttt gtcgagtcag caacagttca agttgtttat ccgtcatggt gatttgctca
    26701 aagaaccggt taaaggggaa tttagtagtt atggattaag cgcaaccgcc actattccct
    26761 ggttttgatg ctttgcaatt tttaacaggc ctggccatgt ggttgggcct ttttatgcgc
    26821 gccatgccca ccgcatacaa aaaaaagccc gtactttcgt acgagctctt ctttaaagat
    26881 ggcggtgagg cggggattcg aaccccggat acgttgccgt atacacactt tccaggcgtg
    26941 ctccttcagc cactcggaca cctcaccaaa ttgttttgct gccagacctc ataggtggca
    27001 acggggcgct actataggga gttggagtaa aacggtcaag aagaatttat atagattgat
    27061 tcgtttggtt acgcaatgaa cacactgttg gtgggacgga gattatgact gtatgtgttc
    27121 tggtcaattg tttatcaaaa gctatgcaga aaatatgaga ttgaagaaat accaaatcgt
    27181 cccttttttt agggcgaaat gtaactcatt gattttgttt actgctattt tgaagtctgg
    27241 aaaaaggggg tgaatctgcg attttgtaag ttttaacagt aaatcaatcg gatagtctgc
    27301 tattattcca gttcactgcc gtacaggcag cttagaaatt ttaatttttc cagataattt
    27361 tggcgagtct gttcactgcc gtacaggcag cttagaaatt tcccggcagg catggcaagt
    27421 tacgtctccc gttcactgcc gtacaggcag cttagaaatt tctgctgtta tcgccgggtt
    27481 gcacgagtgc gttcactgcc gtacaggcag tcttcaaact caacactcca tcctctaata
    27541 tttattcccc ataactcata gacgcaaaaa aggccggtta aaccgacctt ttactcattc
    27601 tttctcttcg cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt
    27661 gctcaggtcg tacggggtca gttcaacagt cactttgtcg cccgtcagga tgcggatgta
    27721 gtttttgcgc attttaccgg agatgtgtgc agtaaccacg tgaccgtttt ctaactctac
    27781 gcggaacatg gtattaggca acgtttcaag aacggtacct tgcatttcaa tattgtcttc
    27841 tttggccatc taatcctctg gggtatcact accgtaattt gaaccggcaa gataatgccg
    27901 aagttctgta aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt
    27961 actccgaaaa cacatggcta agccgcacca aaagcgcaac gtataaggga gcggtgagat
    28021 aaacgatggg cgttacctga cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa
    28081 ccaactctgc gaccgcaatt ataacactct ggggagaaat gtgccgaaaa cattcattct
    28141 tgtggtgaaa acaagcatcg aggtacccag aaattattcg gcaatcgtcc gaggcgcatt
    28201 tgattgagat aattaaggta atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg
    28261 tgatcgttaa ggacctggca gtcgataagt ttaccgccat gacggataaa ttcatcacag
    28321 aagaccagaa gcgccgtttt ggacgcattt tccatccggc tgaacatgga ctcgccacaa
    28381 aatagtgttc cctgggccac gccgtacata ccgccgacaa gctcatcttc acgccagact
    28441 tcaatggagt gggcatgacc gagttcgtga aggcgatggt aggcttcgac cacgccacgc
    28501 gtgatccagg ttccttcttc gcgatcgctg gcacagcctt cgatgacctg accaaaagcg
    28561 taattcatcg tgacacgata gggtgagcgt ttatgaaatc gcttcatact acggctgata
    28621 tgcagtgatt ctggccataa caccgcgcgg ggatcgggcg accaccagag gatggggtcg
    28681 ccaggagaaa accacgggaa aataccacgc tgataagcca ttaataggcg cgcagggcta
    28741 agatcgcccc ccagtgccag caggccgtta ggctcacgta atgcgccttc cggggaaggg
    28801 aaggctattg aatggcgaga aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt
    28861 cggatcgttc aataatagct tacaaaccct gcttaaactg gtaataacgc ccctgtctgg
    28921 caagcagttc tgcgtgagta ccttgctcaa taatttgccc gttgtccatc actattattt
    28981 gttggaaacg agagagtccg cgaagtcgat gggtgaccat taacaccgtt ttctcgcaca
    29041 tcatttctgc aagcaattca aggatctgac tttcggttgt ggcatctaag ccttctgtag
    29101 gttcatccag caataccagc ggcgcatcat gtaacagcgc acgggcgata gccagacggc
    29161 gcagttcacc accggagagc tggcgtccgc cttcacctaa ccaactgttg agacctgcat
    29221 cctcgagcag cttctccaga ccaacgcgac gcaagatctc cgccagagcc tcatcactac
    29281 taccaggcga ggcgagtaac agattatcac gcagcgtggc gctaaacaga tgcactcgct
    29341 ggggaacaac gctgatggtc tgtcgtagag ccgcttcatt caggctggct atgggactat
    29401 cgttaagcaa aatctcgccc tgttgcgggt cccatgcgcg ggtcagcagt tgtaacagtg
    29461 ttgatttgcc gcatccggtt cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa
    29521 gataaatccc tttaagtgcc tgttgggatt gctccggata agtgaactga acatcccgta
    29581 atgtcagcga aacgcgatcg gcaacacgag tttgggtatc agggaaggtg acttccggtt
    29641 tttgatccgt taaatcagtg atccgtacgg cagaggcaat gacttgcccc agatgctgaa
    29701 atgcacccgt tactggtgcc agcgcttcaa acgcggctaa cgcgcagaag acaaacaggg
    29761 caattaacgc gccgggttga gcattgccgc caacgccacc ggacgccatc cacagcatca
    29821 ggatcaccgc taacgcgcca atgagcagca ttatcgcttg cgacaatgcg gtcagttcag
    29881 attgacggcg ttgcgcttcc agccactgaa tttctgtatt ctctaattgc gtgcgataac
    29941 gatcgctggc accaaaaatg gtcagctcag cttgcccttg cagccaggcc gtcagttgtt
    30001 ggcgatactg cccgcgaaga tgagtcagat tttgcccggt gctttttccc gcacgataaa
    30061 acagtggtgg catcaggaaa agcgtcagta acataatgcc gcccagcgta aaggcgaggg
    30121 tgaaatcaag gaaacttaac ccgattgtca ccaccataat caccacaaaa gcacctacca
    30181 gcggcgagat aacgcgcagg taaagatgat cgagcgtatc aacatccgcc accacgcggt
    30241 tgagtaattc accctgacga tagcgcgcca gtccggcagg ggagaggggc agcaatttgc
    30301 tgaaggtgta tatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac
    30361 gttcaaaata acgcccggca gtacgggtga ttgctgcgcc acgcacgccc gcagcgggta
    30421 gcatatagtt gaagctgtac agtccggcaa cccccgctac cgctgaggcg gagaggaacc
    30481 agccggaaag ggtcaacaga ccgatactgg cgagcagcgt gacaattgcc agcacgatac
    30541 caagacttaa catccattta tgacgtttat acagtgccag atagggtagc aaagcgcgca
    30601 tttaaatctc ctcctgacga tgggccagta atgtggcgaa tgggccgcca gccacactta
    30661 attccgcgta acgtccttgc tcaataatct gaccatcctg cattacccaa atgacatccc
    30721 agtcagcaag atcttctaac tggtgggtga ccattaacgt tgtctggcgc agagaggcgg
    30781 cattcagcgc ctccattacg cgctgttcac tgtgagcatc aaggctggca gcgggttcat
    30841 ccaacagtaa tagcgaacag ggatttagta acgcacgggc caccgccacg cgctgcgcct
    30901 gccccacgga aaggcgggca gcctggtcgc caacaggcgt atcaacgcct tgtggcagga
    30961 gcgttagaaa ctcactgacc caggcgttat ccagcgctgt ttgtaactct tgttcgctgg
    31021 catcaggtcg cgccagtagt acgttatccc gtaatgttgc tgccggtaat tgtgggtttt
    31081 gcccaaccca ggagagatgt ttacgccatg attctggtga taaatcgcgt aattctatcc
    31141 cgttgattcg tagcgatccc tgatatgaga gaaaaccaga aagcgcgttc agcagtgaac
    31201 ttttacctga accgctgcga ccaaccaaca ctgctcgttg gcttgctggc aaagtaaaat
    31261 tcagcggtcc ggccagcgtt ttaccttccg gcgacgtgat aaacagatcc tcggcttcaa
    31321 tggtcaccgg atcggtcgat gctaattccg cctcaccgcg ctgcggatgg gcgagcgggg
    31381 tttccataaa cgttttcaaa ctgtcagctg caccaacagc ctgggcttta gcatgataaa
    31441 acgtaccgag atcgcgtaat ggctggaaaa actctggcgc aaggatcagg gccagaaaac
    31501 ccgcagccag cgtcacacca gtatcgtagt gaccaaaatc cagctcgccg aggtaggaaa
    31561 aaccaaagta gaccgccacc agagcaatcg acagcgaggt aaaaaattcg agaatgccgg
    31621 aggataaaaa cgccagccgt agcacttcca ttgtccgttg gcggaaatct tccgaagcag
    31681 aacgaatact ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc
    31741 gcaggcgatc gaggaaatgc ccacttaagc gagcaagagc gagaaagtta cgtcggttag
    31801 catcggcagc ccccattcca accagcgcca taaataacgg aattagcggt gcagtgccca
    31861 gcagaatgag cgccgcagcc cagttagagg ggaagatagc caccacaatc agcagcggca
    31921 ccgacactgc cagcgccatt tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa
    31981 tttgctcgag caccagcgtc gcccagctcc ccgcaggttt accctgaatc cacgctggcc
    32041 ctgcttgttg cagacggtca agaacctgac ggcggatggc aaagcggata tgctgcccgg
    32101 cgtgataacc cacccgttcg cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa
    32161 acagtaacgt aaagggaagc agcagggctt cacggggaat attctccata atcatatgtt
    32221 gcagaattcg cgccatgaac caggcctggg caatgatcaa tatgccgctc acaaagccca
    32281 gcagacgaga aatattcagc caacgttggg agatgacgct ttgctgtttt aaccagcggg
    32341 ttaattcttt ttgacgggat ttattcattg cacgcttagc aggtgagtta tcagaattat
    32401 ttgcagagca atgttacaac ggggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc
    32461 cttctttact tttgttactg atttgtaaaa ttattttgcg tcagctaaac cgtcgaggta
    32521 gcgttccgca tcaagagctg ccatgcagcc tgtaccagca gatgtaatgg cctggcgata
    32581 aatgtgatcc atcacgtcgc ctgcggcaaa gacgccaggg atgctggtct gggtggcatt
    32641 accatgaata cccgactgta ctttgatgta gccgttttcc agttccagct gcccttcgaa
    32701 aatggcagta ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt cgagtgactc
    32761 gatgttatcg ctgttttgcg tatcgcgcag acgaacgcca gtgacaccca tctgatcgcc
    32821 ggtcacttct tccagcgtac ggttggtgtg cagaataatg ttgccgttct ccactttatc
    32881 catcagacgc ttaatgagga ttttttccgc acggaaacca tcacggcggt gaatcagatg
    32941 aacttccgaa gcgatgttag acagatacag cgcctcttca accgcggtat tgccgccgcc
    33001 gatgaccgca actttctggt tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac
    33061 tccacggcct ttaaaggctt cttcagaggg caggccgaga tagcgtgcag aagctccggt
    33121 ggcaataatc agcgcgtcgc aagtgtattc gccgctatcg ccagtcagac ggaacggacg
    33181 attctgcaaa tcaactttgt tgatgtgatc aaagatgatt tcagtttcaa atttggtggc
    33241 atgttcgtgc atgcgcgcca ttaataacgg accggtcaga tcgtttggat cgccaggcca
    33301 gttttccact tccgtggtgg tggtcagttg gccgcctttt tccatgccgg taatcagcac
    33361 aggttgcagg ttggcgcgcg ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc
    33421 caggataagc agtttactgt gtttggtcgt gcccatgaga tccccatagt tgttggcaga
    33481 caatggcagg attgtaggga atttacagac gtaaaaaaag agtatgacga ttttgttaac
    33541 aatttgtgca atcggcagca tcgataagca ggtcaaattc tcccgtcatt atcacctctg
    33601 ctacttaaat ttcccgcttt ataagccgat taaatgatga ataaacgccc ctgttaatga
    33661 atatctggca tgttgtacta aaaatcgatg ttttgctttg acaatcccct ggtgttttgc
    33721 gaaaacattc gaggaagaaa aaaaacagta ttcttatatg cgcataacca tgcatgtaaa
    33781 taccatgttt accgtgctag tgaaatctac gtatggcgtg gacagacgcc attcgtgatg
    33841 tcgatagctg ccacaaggca acggtcttct caccgtagac ccaggcattg cgcgccgtga
    33901 atcttcatga tttcggtcta tcgtgacggg tagcgactct gaacagtgat gtttcagggt
    33961 cagacaggag tagggaagga atacagagag acaataataa tggtagatag caagaagcgc
    34021 cctggcaaag atctcgaccg tatcgatcgt aacattctta atgagttgca aaaggatggg
    34081 cgtatttcta acgtcgagct ttctaaacgt gtgggacttt ccccaacgcc gtgccttgag
    34141 cgtgtgcgtc ggctggaaag acaagggttt attcagggct atacggcgct gctgaacccc
    34201 cattatctgg atgcatcact tctggtattc gttgagatta ctctgaatcg tggcgcaccg
    34261 gatgtgtttg aacaattcaa taccgctgta caaaaacttg aagaaattca ggagtgtcat
    34321 ttagtatccg gtgatttcga ctacctgttg aaaacacgcg tgccggatat gtctgcttac
    34381 cgtaagttac tgggggaaac cctgctgcgt ctgcctggcg tcaatgacac acggacatac
    34441 gttgttatgg aagaagtcaa gcagagtaat cgtctggtta ttaagacgcg ctaacacgga
    34501 acaggtgcaa aatcggcgta ttttgattac actcctgtta atccatacag caacagtact
    34561 ggggtaacct ggtactgttg tccgttttag catcgggcag gaaaagcctg taacctggag
    34621 agcctttctt gagccaggaa tacactgaag acaaagaagt cacattgaca aagttaagta
    34681 gcggtcgccg tcttctggag gcgttgctga tccttattgt cctgtttgcc gtctggttga
    34741 tggctgcctt actaagtttt aacccttcgg accccagctg gtcgcaaacg gcctggcatg
    34801 aacctatcca taatttaggt gggatgcccg gtgcgtggtt ggcagatact ctgttcttta
    34861 tttttggcgt gatggcttac accattcccg tcattattgt cggcggttgt tggtttgcct
    34921 ggcgtcatca gtccagcgac gaatacattg attattttgc cgtttcgcta cgcatcattg
    34981 gcgttttggc gctcatcctt acctcctgtg gtctggcggc aatcaacgct gacgatatct
    35041 ggtattttgc ctccggtggc gtcattggca gcttactaag cactacgcta caaccactgc
    35101 tacacagtag cgggggaact attgcgctgc tctgcgtttg ggcagcgggc ctgacgctgt
    35161 tcaccggttg gtcatgggtg accattgctg aaaaactcgg cggctggatt ttaaatattc
    35221 ttaccttcgc cagtaatcgt acccgtcgcg atgatacctg ggtcgatgaa gatgagtatg
    35281 aagacgacga agagtatgaa gatgaaaatc acggcaaaca gcatgaatca cgccgtgccc
    35341 gtattcttcg cggcgcgcta gcgcgtcgta aacggttggc ggaaaaattc attaatccga
    35401 tggggcggca aacagatgct gcgctgttct ccggtaagcg gatggatgat gaagaagaga
    35461 ttacctacac tgcacgcggt gtggctgccg accctgacga cgtcctgttt tcgggcaatc
    35521 gtgcaacgca gccagaatat gacgaatacg atccattatt aaacggtgcg ccaattaccg
    35581 aacctgttgc tgtagcagct gctgctacca cggcgacaca aagctgggct gcaccggttg
    35641 aacctgtgac tcagacgccg ccagttgcct ctgttgatgt tccacctacg caacctacag
    35701 tagcctggca gcctgtaccg gggccgcaaa cgggagaacc ggttattgca cctgcaccgg
    35761 aaggttaccc acagcagtca caatatgcgc agcctgcagt gcaatataat gagccgctgc
    35821 aacaacctgt acagccacag cagccgtatt atgcgcctgc ggctgaacaa cctgtgcaac
    35881 aaccttacta cgctcctgcg gcggaacaac ctgttcagca accgtattac gcccctgcgc
    35941 cagaacaacc ggtggcaggt aacgcctggc aagccgaaga gcagcaatcc acttttgctc
    36001 cacagtctac ataccagacg gagcaaactt atcagcagcc agctgctcag gagccgttgt
    36061 accaacagcc gcaacccgtt gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag
    36121 agacaaaacc cacgcgtccg ccgctttact attttgaaga agtggaagag aagcgagccc
    36181 gtgaacgtga acaacttgcg gcctggtatc aaccgattcc agaaccggtt aaagaaccgg
    36241 aaccgatcaa atcttcgctg aaagcacctt ctgttgcagc agtacctcca gtagaagccg
    36301 ctgccgctgt ttctccgctg gcatctggtg tgaaaaaagc gacactggcg acgggggctg
    36361 ccgcaaccgt tgccgcgcca gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg
    36421 tcaaagaggg gattggcccg cagttgccac gaccgaaacg catccgcgtg ccaactcgtc
    36481 gtgaactggc gtcttacggt attaagctgc cctcacagcg tgcggcagaa gaaaaagccc
    36541 gtgaagtcca gcgtaatcag tacgattctg gcgatcagta caacgatgat gaaatcgatg
    36601 cgatgcagca ggatgaactg gcacgtcagt tcgcccagac gcagcagcaa cgctatggcg
    36661 aacagtatca acatgatgtg cccgtgaaca cagaagatgc agatgctgcg gcagaggcag
    36721 aactggctcg tcagtttgct caaactcaac aacaacgtta ttccggcgaa caaccggctg
    36781 gggcgaatcc gttctcgctg gatgattttg aattttcgcc aatgaaagcg ttgctggatg
    36841 atggtccaca cgaaccgctg tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac
    36901 cggtagcacc gcagcagcaa tatcagcagc cgcaacaacc ggttgcaccg cagccgcagt
    36961 atcagcagcc gcaatatcag cagccacaac agccggttgc acagcagccg caatatcagc
    37021 agccgcaaca accggttgca ccgcagcagc aatatcagca gccgcaacag ccggttgcac
    37081 agcagccgca atatcagcag ccgcaacagc cagttgcgcc gcagccgcat gataccctgc
    37141 ttcatccgct gttgatgcgc aatggtgaca gccgtccgtt gcataaaccg acgacgccgc
    37201 tgccttctct ggatttgctg acacctccgc cgagcgaagt ggagccggta gatacctttg
    37261 cgcttgaaca aatggcgcgc ctggtagaag cgcgtctggc tgatttccgt attaaagccg
    37321 atgttgtcaa ttactctccg gggccagtta tcactcgctt tgaattgaac ctggcaccgg
    37381 gcgtaaaagc agcgcgcatt tctaacttgt cacgtgacct tgcccgttca ctttcgacgg
    37441 tggcggtgcg tgtcgttgaa gttattcctg gcaaacccta tgtaggtctg gagttaccga
    37501 ataaaaaacg acaaaccgtt tatctgcgcg aagttttgga taacgccaaa ttccgcgata
    37561 atccgtcgcc attaaccgtg gtgctgggta aagatatcgc cggtgagccg gtggttgccg
    37621 atctggcgaa aatgccgcac ttgttggttg cggggactac cggttccggt aagtctgtcg
    37681 gggtgaacgc gatgatcctg agcatgcttt ataaagcaca gccagaagat gtgcgtttca
    37741 tcatgatcga cccgaaaatg ctggagcttt cggtttatga aggcattccg catctgttaa
    37801 cggaagtcgt taccgatatg aaagatgccg ccaacgcgct gcgctggtgt gttaacgaga
    37861 tggagcgtcg ttataaactg atgtctgcgc tgggtgtgcg taatctggcg ggttataacg
    37921 aaaaaattgc tgaagccgat aggatgatgc gtccgattcc agacccgtac tggaagccgg
    37981 gtgacagtat ggatgcccag catccggtgc tgaaaaaaga accgtacatt gtggtgttgg
    38041 tggacgaatt tgccgacctg atgatgacgg taggtaaaaa agtggaagag ctgatagcac
    38101 gtctggcgca aaaagcccgt gccgcgggta tccacctcgt actggcaacg cagcgtccat
    38161 cggttgatgt tattactggt ctgattaaag cgaatattcc gacccgtatc gcctttaccg
    38221 tatccagtaa gattgactca cgtaccattc ttgatcaggc tggtgcggaa tcactgctgg
    38281 gtatggggga tatgctctac tctgggccga actccacgtt gccggtacgt gtccatggtg
    38341 cttttgttcg cgatcaggaa gttcatgccg tggtgcagga ctggaaagcg cgtggtcgcc
    38401 cacagtatgt tgatggcatc acctccgaca gtgaaagcga aggtggcgtg ggcggtttcg
    38461 atggcgctga agaactggat ccgttgttcg atcaggcggt gcagtttgtc actgaaaaac
    38521 gcaaagcgtc aatttctggc gtacagcgtc agttccgcat tggttataac cgtgcagcgc
    38581 gtattatcga acagatggaa gcgcagggga ttgtcagcga acaggggcac aacggtaatc
    38641 gtgaagtgct ggccccaccg ccatttgact aattaatgca acgtagaccg gataagacgc
    38701 gatagcgtcg catccggtat tctatcaact gaaaattcag tattttcttc tttcctcaag
    38761 ctgattatta gcctggaata gagagtagag ggaactcccg attgggagtg acgtaatttg
    38821 aggaataatg atgaaaaaaa ttgccatcac ctgtgcatta ctctcaagct tagtagcaag
    38881 cagcgtttgg gctgatgccg caagcgatct taaaagccgc ctggataaag tcagcagctt
    38941 ccacgccagc ttcacacaga aagtgactga cggtagcggc gcggcggtgc aggaaggtca
    39001 gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgactc aacctgatga
    39061 aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc
    39121 tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa
    39181 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgttctgac
    39241 gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac
    39301 aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc agctgaaatc
    39361 ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac
    39421 ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg
    39481 ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg
    39541 gccagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatc
    39601 tgcattctat gatcctctgg gggccgccgg gtaccggcaa aacaaccctc gctgaagtga
    39661 ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggtgtgaaag
    39721 agattcgcga ggcgatcgag cgcgctcggc aaaaccgcaa tgcaggtcgc cgcactattc
    39781 tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata
    39841 ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta
    39901 attcggcact gctttcccgt gcccgtgtct atctgttgaa atccctgagt acagaggata
    39961 ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt ggtcaggata
    40021 ttgttctgcc agatgaaaca cgacgcgcca ttgctgaact ggtgaatggc gacgcgcgcc
    40081 gggcgttaaa tacgctggaa atgatggcgg atatggccga agtcgatgat agcggtaagc
    40141 gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc cgctttgata
    40201 acaaaggcga tcgcttttac gatctgattt ccgcactgca taagtcggta cgtggtagcg
    40261 cacccgatgc ggcgctctac tggtatgcgc gaattattac cgccggtggc gatccgttat
    40321 atgtcgcgcg tcgttgcctg gcaattgctt ccgaagatgt cggcaatgcc gacccacggg
    40381 cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg
    40441 aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct
    40501 acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg tccggattat gacgtgccgg
    40561 ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc
    40621 gttacgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa
    40681 tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag attggcgaaa
    40741 agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt
    40801 atcgttgcgg taatgttgtt aatgtatccc tgtggtcgca ggctgtggcc acatctccca
    40861 tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag
    40921 acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg
    40981 ctcttgagga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta
    41041 actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac
    41101 gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt
    41161 tacaggctga aattcgcgat attgcgctaa ccatccctaa cctgcctgca gatgaagtgc
    41221 cggtaggtaa agacgaaaat gacaacgttg aagtcagtcg ctggggcacc ccgcgtgagt
    41281 ttgactttga agttcgtgac catgtgacgc tgggtgaaat gcactctggc ctcgactttg
    41341 cagccgcagt taagttgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca
    41401 tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg
    41461 agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga
    41521 aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact
    41581 atgcgctgat cccaacggca gaagttccgc tgaccaacct ggtacgcggt gaaatcatcg
    41641 atgaagatga tctgccaatt aagatgaccg cccacacccc atgtttccgt tctgaagctg
    41701 gttcatatgg tcgtgacact cgtggtctga tccgtatgca ccagttcgac aaagttgaaa
    41761 tggtgcagat cgtgcgcccg gaagactcaa tggcggcgct ggaagagatg actggtcatg
    41821 cggaaaaagt cctgcagctg ctgggcctgc cgtaccgtaa aatcatcctt tgtaccggcg
    41881 acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca
    41941 cctaccgcga gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg
    42001 cacgttgccg tagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg
    42061 gtcttgctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc
    42121 gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat
    42181 acccaatttt tctgaatcta aaaagcgcct gcgggcgctt tttttgtctc cctttgatac
    42241 cgaacaataa ttactcctca cttacaagta atactacttt cgagtgaaaa tctacctatc
    42301 tctttgattt tcaaattatt cgatgtatac aagcctatat agcgatctgc tatagaaata
    42361 attacacaat acggtttgtt actggaatca atcgtgagca agcttgagtg agccattatg
    42421 aaaacgaaaa tccctgatgc ggtattggct gctgaggtga gtcgccgtgg tttggtaaaa
    42481 acgacagcga tcggcggcct ggcaatggcc agcagcgcat taacattacc ttttagtcgg
    42541 attgcgcacg ctgtcgatag cgctattcca acaaaatcag acgaaaaggt tatctggagc
    42601 gcctgtacag ttaactgtgg tagtcgctgc ccgctacgta tgcacgtagt ggacggtgaa
    42661 atcaaatatg tcgaaacgga caataccggc gatgacaatt acgacggcct gcaccaggtt
    42721 cgcgcctgtc tgcgtgggcg ttccatgcgt cgccgtgtct acaatccgga ccgcctgaaa
    42781 tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa
    42841 gaagcctacg acatcatcgc gaccaaaatg cagcgcctga tcaaagagta cggcaacgag
    42901 tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcgtggccg
    42961 ccgggaaata ccctggtcgc gcggctaatg aactgctgcg gcggttatct gaaccattat
    43021 ggcgactact cctccgcgca aattgcggaa ggtctgaact atacctacgg cggctgggca
    43081 gatggtaaca gcccgtcgga tatcgaaaac agtaaactgg tagtgctgtt tggtaataac
    43141 cctggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcgcgccag
    43201 aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgt
    43261 gaagatgagt ggatccctat tcgtccggga acagatgccg cactggttaa cggtctggcg
    43321 tatgtcatga tcaccgaaaa cctggtagat caggaattcc tcgataaata ttgcgttggc
    43381 tacgacgaga aaaccctgcc agccagtgcg ccgaaaaatg gtcactataa agcttatatt
    43441 ctgggtgaag gacccgatgg cgtggctaaa acaccggaat gggcctcgca aatcaccggt
    43501 gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt
    43561 atcagccagg gatggggccc gcagcgtcat gctaacggtg aaatcgcaac ccgtgctatc
    43621 tcgatgctgg cgattctgac cggtaacgtt ggtattaacg gggggaacag cggcgcgcgc
    43681 gaaggttcat acagcttacc gtttgtccgt atgccgactc tcgaaaaccc gatccagacc
    43741 agcatttcta tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg
    43801 cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat
    43861 gccggtaact gcctgattaa tcagcattct gaaatcaacc gtacccatga aatccttcag
    43921 gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa
    43981 tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcactggat
    44041 gcatcctgcg ggaatatgtc ttacgttatt ttcaatgatc aggtcattaa accgcgcttt
    44101 gaatgtaaga ctatctatga gatgaccagc gaactggcaa aacgtcttgg cgtcgaacaa
    44161 cagtttactg aaggtcgtac tcaggaagag tggatgcggc atctgtatgc ccagtcgcgg
    44221 gaagcgattc ctgaactgcc aacgtttgaa gagttccgta agcaggggat ctttaaaaag
    44281 cgcgatccgc aagggcatca cgttgcttac aaagccttcc gtgaagatcc gcaggctaat
    44341 ccattgacca cgccatcggg caaaattgag atctattcac aggcgctggc tgacattgcc
    44401 gctacctggg aattgccaga aggtgatgtg atcgatccac tgccgatcta tacgccgggc
    44461 tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac gggtttccac
    44521 tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc agcttgccgt
    44581 caggaaatgt ggatcaaccc gcttgatgcg caaaaacgcg gtatcagcaa cggcgataaa
    44641 gtcaggatct ttaatgatcg tggtgaggtt catattgagg cgaaagtgac gccacgaatg
    44701 atgccgggtg tggtcgcact gggtgaaggt gcctggtatg acccggatac aaaacgtgtc
    44761 gataagggcg gttgtattaa cgtactgacc actcaacgtc cgtctcctct tgccaagggg
    44821 aatccatcac atacgaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac
    44881 ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc
    44941 ttgtaaagac tacaaagatt tgacgccaga agtcagcttc cgccgcattt atgaatatgc
    45001 tggtggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct
    45061 gtcgatttca tgtaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc
    45121 gatgcataaa cgtgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg
    45181 ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat
    45241 gaccaaatgc gatggttgtt atgaccgtgt tgcggaggga aaaaaaccga tctgcgtaga
    45301 gtcctgcccc ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg
    45361 cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat
    45421 caaacccaat gccaacagcc gcccaaccgg ggataccact ggctatctgg caaacccgaa
    45481 ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg
    45541 ggcaatgtgt agcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc
    45601 gagcagaagc ccagcagcgt gttatcgcct gcatgttggg tttatggggg ctgatgggca
    45661 ttggctttat cgcctctatg cttcatcttg gttcaccgat gcgcgctttt aactcgctca
    45721 atcgggtagg ggcttcagca ctcagtaacg aaattgccag cggttcgatc ttttttgctg
    45781 taggcggcat cggctggttg ctggcaatgc tgaaaaagct ttcaccggca ttgcgtacgc
    45841 tttggctggt ggtgacgatg gttcttggcg tagtctttgt ctggatgatg gtgcgtgtgt
    45901 ataacagcat cgatacagta cccacctggt acagcatctg gacaccgatg ggcttcttcc
    45961 tgacgatgtt tatgggcggc ccgttactgg gttacctgtt gctgagtctg gctggtgtcg
    46021 atggctgggc gatgcgtctg ctgccagcca tttctgttct ggcactggta gtgagtggcg
    46081 tgatgtcagt gatgcagggc gcagagctgg cgaatattca tagctctgtg cagcaggccg
    46141 cagcgctggt gccggactat ggtgcgctga tgtcctggcg gatcgtgctt ttggccgttg
    46201 ccctgtgctt gtggattgca ccacagctaa aaggttatca gcctgaggtt ccgctacttt
    46261 ctgtatcatt cattctgctt ctggcagggg aattaatcgg tcgtggcgta ttctatggtt
    46321 tgcatatgac cgtggggatg gctgtcgcaa gttaatcagt aacaaccggg gtttcggccc
    46381 cggttcgctt ttatttctgc ttcgttaacg tgtcataact ggtcatcaag ttacgataat
    46441 caggaatgtg gttagagaac aacgtcgcca gtccttcaat atcattacgc cagtcacgat
    46501 gcagctcgca cgccacgccg aaccatgtca tcaattgcgc accagcttgc gacatgcgat
    46561 cccatgccga atgccgggta atttcattaa aggtccctga cgcgtcagtc acgacaaaga
    46621 cgtcaaaccc ttcttcaatg gctgaaagcg ccgggaatgc cacgcaaact tcggttacca
    46681 caccggcaat aattaactgt tttttacctg tcgctttgac agcttttaca aaattttcgt
    46741 tatcccaggc gttaatattt cccgggcgag caatgtaagg tgcatcgggg aattgtgctt
    46801 tcagttctgg aactaatggg ccgttaggac cggtttcaaa actggtggta agaatagttg
    46861 gtaagttgaa atacttagcc aggtcaccca gcgccagcac gttattttta aacttatcgg
    46921 gttcgatatc ccgtacaagg gaaagtaaac cagcctggtg atcaacaagc aaaacggcag
    46981 catcattttt atcaagacga acatacggtt tggtcatcct cttctccttt cgagagtcag
    47041 tggtacgtct aaatcggtca gacgcgatgc attgctctga aagcatagac gggaaatatg
    47101 agtttgctgt gaccatgaaa tttttcgact gaacgaagcg tatgaaattt gtgttagatc
    47161 aataaaaaca atcagatgag attaatcgtc tcttcagcat tagcgttatg aataagtaat
    47221 ataaatcgat aataccgccg ctgcgatatg cgcgtatttt cacccattga caatgttttt
    47281 ggcggtggca tgatgcgcat gaaatttgaa cttcctcacg gttttaattc atgtccacgt
    47341 atacccggcc tgtcatgctt ttgctgtctg gcctgctttt gttgactctg gcgattgcgg
    47401 tgttaaatac actcgtgccg ctttggctcg cccaggaaca gatgtccaca tggcaggtag
    47461 gcgttgtcag ctcatcctat tttaccggca accttgtcgg tacattgctg acagggtatg
    47521 tcattaaacg cattggcttt aaccgtagct attatctggc ctccttcatt tttgccgctg
    47581 gctgtgccgg ccttggcctg atgattggtt tctggagttg gttggcgtgg cgttttgtcg
    47641 cgggcgtcgg ctgtgccatg atttgggtag tcgttgagag cgcgctgatg tgcagtggga
    47701 cgtcacgtaa ccgtgggcgt ttgcttgctg catacatgat ggtttattac gtgggaacgt
    47761 ttttaggcca gttactggtc agcaaagtct caaccgagtt gatgagtgta ttgccgtggg
    47821 ttacagcttt gacgttggca ggaatcttac cgctgttgtt tacgcgtgtc ctgaatcagc
    47881 aggctgaaaa ccatgattcg acgtcaatta cgtcaatgct aaaactccgt caggcgcggc
    47941 ttggcgtgaa tggctgcatt atctcaggaa tcgtgctggg atctctatat ggcctgatgc
    48001 cgctgtacct caatcacaaa ggggtgagca atgccagcat tggtttctgg atggcggtac
    48061 tggtcagtgc gggtatcctc gggcaatggc cgattggacg tctggcggat aagtttggtc
    48121 gactgctggt gttgcgtgtt caggtctttg tcgtcattct cggcagtatt gcgatgctta
    48181 gccaggcggc gatggcccca gcgttattca tcctcggtgc cgctggcttt acgctatatc
    48241 cggtggcgat ggcctgggct tgtgagaaag ttgaacatca tcaactggtg gcgatgaacc
    48301 aggccttact gttgagctat accgtgggaa gcctgcttgg cccgtcattt accgctatgc
    48361 taatgcagaa tttctccgat aatttattat ttatcatgat cgccagcgta tcgtttatct
    48421 atttgctgat gctgcttcgt aacgccggtc atacgccgaa acccgtggct cacgtgtaaa
    48481 tgaattcaag cagagtgtga acttactgtt tcacactctg cttttctgtg cacaaaaaag
    48541 cggcaactta ataaagttgc cgctttacgg ggaaattaga acatgacctt atgaccgtac
    48601 tgctcaagaa tgcctttcac gcgttccatg gtctctttct tcggtggttt aacaccatcg
    48661 agtttgtatt cttcactcat tgccacccat ttgtgtttgc ccagttcgtg gtaggggagg
    48721 agctcgattt tctcaacgtt gcccatatca cgggtaaact caccaaggcg atgcgctgaa
    48781 tcgtcatcgt cagaccagcc tgggacaaca acatagcgga tccacacctt cacatttttg
    48841 ttcgccagat atttagcgaa ctccagcgtg cggtggttgg aaactccaac cagattttgg
    48901 tggatctcgt cgttcatctg tttgagatcg agcattacca ggtcggttac ttccagcagt
    48961 tcatcaatca ccggatcgta acgacgaaca aaaccgttgg tgtccagaca ggtatgaatg
    49021 ccttcttttt tgcaggcgcg gaaccagtca cgaacaaact cagcttgtag gattgcctca
    49081 ccgccggatg cggtaacgcc gccgccggaa gcgttcataa agtggcgata ggtcaccact
    49141 tccttcatca aatcttcaac ggtaacttct ttaccgccat gcgtatccca ggtgtcgcgg
    49201 ttatgacaat acaggcagcg catcaggcag ccctggaaaa aggtaataaa gcgaataccc
    49261 gggccgtcta cggttccaca ggattcaaag gagtgaatgc gaccaataac tgacattgcg
    49321 gtgtttctcc agatgtggcc catctgaggc cgtgttggtg cgcagctcga aggctacgtc
    49381 gagtctgttt tggcagtcac cttaaagtat agatagctga caaaaaaggc tctcgtgcta
    49441 aaaaaggccc cactttcgtg gagcctttat tgtacgcttt ttactgtacg atttcagtca
    49501 aatctaatta catagattga gtgaaggtac gagtaataac gtcctgctgc tgttctttag
    49561 tcagcgagtt gaaacgtact gcgtagccag atacacggat ggtcagctgc ggatattttt
    49621 ccgggttttc catcgcgtcg agcagcattt cacggttcat cacgttaacg ttcaggtgct
    49681 gaccaccttc gatggatgct tcgtggtgga agtaaccatc catcagacca gccaggttgg
    49741 tcttacgaac ttcgtcgtct ttacccagtg cgttcggaac gatagagaag gtgtaggaga
    49801 taccatcttt agcgtaagcg aacggcagtt tagcaacgga agtcagagac gctacagcac
    49861 ctttctggtc acgaccgtgc atcgggttag ctcccggtcc gaacggcgcg ccagcacgac
    49921 gaccgtctgg ggtgttacca gttttcttac catacacaac gttagaagtg atggtcagaa
    49981 cagactgagt cgggatagcg tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac
    50041 gttctaccag gtcaacagcc aggtcatcta cacgcggatc gttgttacca aactgcgggt
    50101 attcgccttc gatttcgaag tcgatagcca gaccgtcttc gtcacgaatc ggtttaactt
    50161 tcgcatattt gattgcagac agggagtcag cagcaacgga cagaccagcg ataccacacg
    50221 ccatggtgcg gataacgtca cggtcgtgca gcgccatcag agaggcttcg tagctgtact
    50281 tgtcgtgcat gtagtggatg atgttcagtg cagtgatgta ctgtttagcc agccagtcca
    50341 tgaagtgatc catgcgctcc atcacttcat catagttcag gacatcgcct ttgatcggtt
    50401 cagacttcgg accaacctgc attttcagtt tttcgtcaac gccgccgttg attgcgtaca
    50461 gcatggtttt cgccaggttt gcacgcgcac cgaagaactg catttgttta ccaacgatca
    50521 tcgggcttac gcagcaagca atagcgtagt catcgttgtt gaagtccgga cgcatcaggt
    50581 catcgttctc atactgcaga gaagaggtgt cgatggacac tttagcggcg aatttcttga
    50641 agttcagcgg cagtttttca gaccacagaa tggtcatgtt cggttccgga gacggaccca
    50701 tggtgtacag ggtgttcagg aaacggaagc tgtttttggt aaccagggta cgaccgtcga
    50761 ggcccatacc accgatagat tcggttgccc agatcgggtc gccagagaac agttcatcgt
    50821 attccggagt acgcaggaag cgaaccatac gcagtttcat gaccaggtgg tcaaccattt
    50881 cctgcgcttc ttgttcggtg atcttgccag ctttcaggtc acgttcgatg tacacatcca
    50941 ggaaggtgga ggtacgaccg aaggacattg cagcaccgtt ctgagactta acagcagcca
    51001 ggtagccgaa gtaagtccac tggatagctt cctgagcgtt ggtagccgga ccagagatgt
    51061 cgtagccgta tttcgcagcc atttctttca tctgacccag agcgcggtgc tgttcagcga
    51121 tttcttcgcg cagacggata gtctgttcca ggtttacgcc gttttccaga tcagcctgca
    51181 gagaggtgaa ctgtgcaagt ttgtctttca tcaggtagtc gataccgtac agcgcaacgc
    51241 gacggtagtc accgatgata cgaccacggc cataagcatc tggcagaccg gtcagaacac
    51301 cggatttacg gcaacgcagg atgtccggag tgtaaacgtc gaacacgccc tggttgtgag
    51361 ttttacggta ttcagtgaag atttttttga tcatcgggtc cagttcgcgg ttgtacgctt
    51421 tgcaggaacc ttcgatcatt ttgataccac cgaacgggat aagagcacgt ttcagcggag
    51481 cttcagtctg cagaccaacg attttctcaa gctgcttgtt gatgtaacca gcgtcgtgag
    51541 aggtgatggt ggaagcaaca gcggtgtcaa agtcaactgg cgcgtgagtg cggttttcca
    51601 gtttaacgcc ttccattact ttgtcccaca gggtggtggt cgcttcagta gcgccagcca
    51661 ggaaggactc gtcaccctcg tacggagtgt agtttttctg aatgaagtca cggacgttta
    51721 cttcattctg ccagtcacct ttggtaaaac cttcccaggc tgtggctaac ttttcattaa
    51781 gctcggacat gtaacaccta ccttcttaag tggatttttt atttactgcg tacttcgaca
    51841 accattaatg gtgatcgttt tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca
    51901 aaccaccgcc gataatatta ccgatcgtaa ccggaatcag gttatcagtg atgaaattca
    51961 tcacggtcag gtgagaaaaa ttttccggtg cagaaccgac agcggtccag aattccggag
    52021 atgcgaagtc gcggattaca atacccatcg ggatcataaa catgtttgcg atactgtgct
    52081 caaaaccgct ggcaacaaac atcgcgaccg gcagcaccat aatgaacgct ttgtccatca
    52141 ggctgcggcc agaataactc atccatactg ccagacatac catcaggttc gccaggatac
    52201 caagacagac ggcctcaata aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt
    52261 ttagtcccca ttgaccattt gcggtcatat actcgccgga aagccacatt aaaagtacaa
    52321 acagcagtgc gccgaccagg ttgccaaaat aaacatttag ccagtttttc gccaactgac
    52381 cccaggtgat gcgcccactc gccttggcaa caacaatcaa cactgtggaa gtaaagagat
    52441 cggctccgca gacaacacaa agaatcaacc ccagagagaa gcaaatgccg ccaaccagtt
    52501 ttgccatgcc gaagggcatt gtacctgtgc cagtggttgc tgtgatatag aagacgaatg
    52561 cgattgagat gaaaacaccg gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg
    52621 ttgctttata gacacctgcc tcttcggcca ctttggccat tgcagcagga agtaaaagat
    52681 caaaagggtt gtcagctttc acactaactc tctctttatt aagtcggcga cgagatacta
    52741 acaaagcatt atagatgaga aattgatata gatcatatct cgcctggctt ataggcccgt
    52801 aactcgcatg gttttt
//
LOCUS       DAZGJT010000022        52666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZGJT010000022 DAZGJT010000000
VERSION     DAZGJT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.022"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            254..820
                     /gene="mntP"
                     /locus_tag="ACHKFK_001430"
     CDS             254..820
                     /gene="mntP"
                     /locus_tag="ACHKFK_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO9051800.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(817..1626)
                     /gene="rlmA"
                     /locus_tag="ACHKFK_001431"
     CDS             complement(817..1626)
                     /gene="rlmA"
                     /locus_tag="ACHKFK_001431"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO9051801.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPLRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVGQLRERLDAKAAAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLKGFTLQQSDELCY
                     LMRLRGDEAVALLQMTPFAWRAKPEVWQALAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1792..2001)
                     /gene="cspE"
                     /locus_tag="ACHKFK_001432"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1792..2001)
                     /gene="cspE"
                     /locus_tag="ACHKFK_001432"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO9051802.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2014..2157)
                     /gene="yobF"
                     /locus_tag="ACHKFK_001433"
     CDS             complement(2014..2157)
                     /gene="yobF"
                     /locus_tag="ACHKFK_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO9051803.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2827..3114)
                     /gene="yebO"
                     /locus_tag="ACHKFK_001434"
     CDS             complement(2827..3114)
                     /gene="yebO"
                     /locus_tag="ACHKFK_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO9051804.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(3189..3332)
                     /gene="mgrB"
                     /locus_tag="ACHKFK_001435"
     CDS             complement(3189..3332)
                     /gene="mgrB"
                     /locus_tag="ACHKFK_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO9051805.1"
                     /translation="MKKFRWVALVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3491..3730
                     /gene="yobH"
                     /locus_tag="ACHKFK_001436"
     CDS             3491..3730
                     /gene="yobH"
                     /locus_tag="ACHKFK_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO9051806.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(3874..4665)
                     /gene="kdgR"
                     /locus_tag="ACHKFK_001437"
     CDS             complement(3874..4665)
                     /gene="kdgR"
                     /locus_tag="ACHKFK_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO9051807.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     MQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4842..6215
                     /gene="yebQ"
                     /locus_tag="ACHKFK_001438"
     CDS             4842..6215
                     /gene="yebQ"
                     /locus_tag="ACHKFK_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051808.1"
                     /translation="MPKVQADGLPLPLRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIGAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLIAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(6261..7142)
                     /gene="htpX"
                     /locus_tag="ACHKFK_001439"
     CDS             complement(6261..7142)
                     /gene="htpX"
                     /locus_tag="ACHKFK_001439"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO9051809.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATGLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7334..9382)
                     /gene="prc"
                     /locus_tag="ACHKFK_001440"
     CDS             complement(7334..9382)
                     /gene="prc"
                     /locus_tag="ACHKFK_001440"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO9051810.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLESDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPSGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDSTRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9402..10100)
                     /gene="proQ"
                     /locus_tag="ACHKFK_001441"
     CDS             complement(9402..10100)
                     /gene="proQ"
                     /locus_tag="ACHKFK_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO9051811.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRSQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10197..10694)
                     /gene="msrC"
                     /locus_tag="ACHKFK_001442"
     CDS             complement(10197..10694)
                     /gene="msrC"
                     /locus_tag="ACHKFK_001442"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO9051812.1"
                     /translation="MNKAEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10824..12107
                     /gene="yebS"
                     /locus_tag="ACHKFK_001443"
     CDS             10824..12107
                     /gene="yebS"
                     /locus_tag="ACHKFK_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO9051813.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGAMVFFCVIGAPLILVTSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12076..14709
                     /gene="yebT"
                     /locus_tag="ACHKFK_001444"
     CDS             12076..14709
                     /gene="yebT"
                     /locus_tag="ACHKFK_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO9051814.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLSSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPGGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLQEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            14789..16228
                     /gene="rsmF"
                     /locus_tag="ACHKFK_001445"
     CDS             14789..16228
                     /gene="rsmF"
                     /locus_tag="ACHKFK_001445"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO9051815.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKTSVADFLQLTAPYGWTLTPIPWCEEGFWIERDSEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCLWLKETYHDAVEFLPLGD
                     LFPGANKALTEDGFLHVFPQIYDCEGFFVARLRKTQAIPVLPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            16346..16582
                     /gene="yebV"
                     /locus_tag="ACHKFK_001446"
     CDS             16346..16582
                     /gene="yebV"
                     /locus_tag="ACHKFK_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO9051816.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16687..16878
                     /gene="yebW"
                     /locus_tag="ACHKFK_001447"
     CDS             16687..16878
                     /gene="yebW"
                     /locus_tag="ACHKFK_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO9051817.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(16879..17535)
                     /gene="pphA"
                     /locus_tag="ACHKFK_001448"
     CDS             complement(16879..17535)
                     /gene="pphA"
                     /locus_tag="ACHKFK_001448"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO9051818.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASRQMSLWLINGGD
                     WFIALADNQQKQAKTALEKCQHLPFILELHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGIAGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(17930..18271)
                     /gene="yebY"
                     /locus_tag="ACHKFK_001449"
     CDS             complement(17930..18271)
                     /gene="yebY"
                     /locus_tag="ACHKFK_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO9051819.1"
                     /translation="MMKNSILAFLLLTSSAAALAAPQVITVSRFEVGKEKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(18284..19156)
                     /gene="copD"
                     /locus_tag="ACHKFK_001450"
     CDS             complement(18284..19156)
                     /gene="copD"
                     /locus_tag="ACHKFK_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO9051820.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLTKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGAGWSDVFSPNIWLAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(19160..19534)
                     /gene="yobA"
                     /locus_tag="ACHKFK_001451"
     CDS             complement(19160..19534)
                     /gene="yobA"
                     /locus_tag="ACHKFK_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO9051821.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            19673..19903
                     /gene="holE"
                     /locus_tag="ACHKFK_001452"
     CDS             19673..19903
                     /gene="holE"
                     /locus_tag="ACHKFK_001452"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO9051822.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            20005..20661
                     /gene="yobB"
                     /locus_tag="ACHKFK_001453"
     CDS             20005..20661
                     /gene="yobB"
                     /locus_tag="ACHKFK_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO9051823.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSYGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEHGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            20684..21346
                     /gene="exoX"
                     /locus_tag="ACHKFK_001454"
     CDS             20684..21346
                     /gene="exoX"
                     /locus_tag="ACHKFK_001454"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO9051824.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(21343..23403)
                     /gene="ptrB"
                     /locus_tag="ACHKFK_001455"
     CDS             complement(21343..23403)
                     /gene="ptrB"
                     /locus_tag="ACHKFK_001455"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO9051825.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     QSAD"
     gene            complement(23612..24271)
                     /gene="yebE"
                     /locus_tag="ACHKFK_001456"
     CDS             complement(23612..24271)
                     /gene="yebE"
                     /locus_tag="ACHKFK_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO9051826.1"
                     /translation="MANWLNQLQSLLGQSRSSTSSSADQGLGKLLVPGALDGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEKGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(24598..24954)
                     /gene="yebF"
                     /locus_tag="ACHKFK_001457"
     CDS             complement(24598..24954)
                     /gene="yebF"
                     /locus_tag="ACHKFK_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO9051827.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(25021..25311)
                     /gene="yebG"
                     /locus_tag="ACHKFK_001458"
     CDS             complement(25021..25311)
                     /gene="yebG"
                     /locus_tag="ACHKFK_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO9051828.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            25445..26623
                     /gene="purT"
                     /locus_tag="ACHKFK_001459"
     CDS             25445..26623
                     /gene="purT"
                     /locus_tag="ACHKFK_001459"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO9051829.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSENLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAHAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(26679..27320)
                     /gene="kdgA"
                     /locus_tag="ACHKFK_001460"
                     /gene_synonym="eda"
     CDS             complement(26679..27320)
                     /gene="kdgA"
                     /locus_tag="ACHKFK_001460"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO9051830.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(27357..29168)
                     /gene="edd"
                     /locus_tag="ACHKFK_001461"
     CDS             complement(27357..29168)
                     /gene="edd"
                     /locus_tag="ACHKFK_001461"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO9051831.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDNNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(29403..30878)
                     /gene="zwf"
                     /locus_tag="ACHKFK_001462"
     CDS             complement(29403..30878)
                     /gene="zwf"
                     /locus_tag="ACHKFK_001462"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO9051832.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            31216..32085
                     /gene="hexR"
                     /locus_tag="ACHKFK_001463"
                     /gene_synonym="yebK"
     CDS             31216..32085
                     /gene="hexR"
                     /locus_tag="ACHKFK_001463"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO9051833.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGTKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            32213..33655
                     /gene="pyk"
                     /locus_tag="ACHKFK_001464"
     CDS             32213..33655
                     /gene="pyk"
                     /locus_tag="ACHKFK_001464"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO9051834.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQEAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(33787..34758)
                     /gene="lpxM"
                     /locus_tag="ACHKFK_001465"
                     /gene_synonym="msbB"
     CDS             complement(33787..34758)
                     /gene="lpxM"
                     /locus_tag="ACHKFK_001465"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9051835.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMVMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(34877..36199)
                     /gene="mepM"
                     /locus_tag="ACHKFK_001466"
     CDS             complement(34877..36199)
                     /gene="mepM"
                     /locus_tag="ACHKFK_001466"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO9051836.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(36215..37147)
                     /gene="znuA"
                     /locus_tag="ACHKFK_001467"
     CDS             complement(36215..37147)
                     /gene="znuA"
                     /locus_tag="ACHKFK_001467"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO9051837.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETQVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHDDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDDNLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLNQLANQYASCLKGD"
     gene            37226..37981
                     /gene="znuC"
                     /locus_tag="ACHKFK_001468"
     CDS             37226..37981
                     /gene="znuC"
                     /locus_tag="ACHKFK_001468"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO9051838.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            37978..38763
                     /gene="znuB"
                     /locus_tag="ACHKFK_001469"
     CDS             37978..38763
                     /gene="znuB"
                     /locus_tag="ACHKFK_001469"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO9051839.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(38908..39918)
                     /gene="ruvB"
                     /locus_tag="ACHKFK_001470"
     CDS             complement(38908..39918)
                     /gene="ruvB"
                     /locus_tag="ACHKFK_001470"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO9051840.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(39927..40538)
                     /gene="ruvA"
                     /locus_tag="ACHKFK_001471"
     CDS             complement(39927..40538)
                     /gene="ruvA"
                     /locus_tag="ACHKFK_001471"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO9051841.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKISRPDASSETLIREALRAAL"
     gene            complement(40677..40742)
                     /gene="yobI"
                     /locus_tag="ACHKFK_001472"
     CDS             complement(40677..40742)
                     /gene="yobI"
                     /locus_tag="ACHKFK_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFO9051842.1"
                     /translation="MCIFITHFFAEYVIFKYLLPI"
     gene            40816..41418
                     /gene="yebB"
                     /locus_tag="ACHKFK_001473"
     CDS             40816..41418
                     /gene="yebB"
                     /locus_tag="ACHKFK_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFO9051843.1"
                     /translation="MNINYPAEYEIGDIVFTCISAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSANQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIEMFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAVGVETPQPELTEAV"
     gene            complement(41420..41941)
                     /gene="ruvC"
                     /locus_tag="ACHKFK_001474"
     CDS             complement(41420..41941)
                     /gene="ruvC"
                     /locus_tag="ACHKFK_001474"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO9051844.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(41976..42716)
                     /gene="yebC"
                     /locus_tag="ACHKFK_001475"
     CDS             complement(41976..42716)
                     /gene="yebC"
                     /locus_tag="ACHKFK_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9051845.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(42745..43197)
                     /gene="nudB"
                     /locus_tag="ACHKFK_001476"
     CDS             complement(42745..43197)
                     /gene="nudB"
                     /locus_tag="ACHKFK_001476"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO9051846.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDASAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(43190..44962)
                     /gene="aspS"
                     /locus_tag="ACHKFK_001477"
     CDS             complement(43190..44962)
                     /gene="aspS"
                     /locus_tag="ACHKFK_001477"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO9051847.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILERTGAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANLTALAELSIQVVKKAENN"
     gene            45272..45838
                     /gene="yecD"
                     /locus_tag="ACHKFK_001478"
     CDS             45272..45838
                     /gene="yecD"
                     /locus_tag="ACHKFK_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO9051848.1"
                     /translation="MPELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            45835..46653
                     /gene="yecE"
                     /locus_tag="ACHKFK_001479"
     CDS             45835..46653
                     /gene="yecE"
                     /locus_tag="ACHKFK_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO9051849.1"
                     /translation="MIYIGLPQWSHPKWVRLGITNLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKEEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTAKNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            46706..47101
                     /gene="yecN"
                     /locus_tag="ACHKFK_001480"
     CDS             46706..47101
                     /gene="yecN"
                     /locus_tag="ACHKFK_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO9051850.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            47142..47885
                     /gene="cmoA"
                     /locus_tag="ACHKFK_001481"
     CDS             47142..47885
                     /gene="cmoA"
                     /locus_tag="ACHKFK_001481"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO9051851.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            47882..48853
                     /gene="cmoB"
                     /locus_tag="ACHKFK_001482"
     CDS             47882..48853
                     /gene="cmoB"
                     /locus_tag="ACHKFK_001482"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO9051852.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(49018..51447)
                     /gene="torZ"
                     /locus_tag="ACHKFK_001483"
     CDS             complement(49018..51447)
                     /gene="torZ"
                     /locus_tag="ACHKFK_001483"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO9051853.1"
                     /translation="MTLTRREFIKHSGIAAGTLVVTSAEPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPICSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGAFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPSGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAAQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYIRYGDFRADPVK
                     NALGTPSGKIEIYSRTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKLGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNALK
                     LTAFDQPAVQA"
     gene            complement(51472..52572)
                     /gene="torY"
                     /locus_tag="ACHKFK_001484"
     CDS             complement(51472..52572)
                     /gene="torY"
                     /locus_tag="ACHKFK_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO9051854.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFEAHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKDGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGDLATVTPGTD
                     LTVVDVSGKQPIVRLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALIDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT        12611 a        13595 c        12861 g        13599 t
ORIGIN      
        1 attctcaaaa tatgttaagg ttgcgccctc atttggggag tagctgattt ccagattccg
       61 gaaatgtacg tgtcaacata ctcgttgcaa aacgtggcac gtacggactg aacactttca
      121 gtcaggcgag accatatgca catcaatcgc tatgcctgca tgaacgcgaa aaccatcgcg
      181 gcttgttgtt catgtatgca tgtttgctgg gggcgatgat gtgttttatg gataccccgg
      241 tcaggacgtt gtcatgaata tcactgctac tgttcttctt gcgtttggta tgtcgatgga
      301 tgcctttgct gcatcaatcg gtaaaggtgc caccctccat aaaccgaaat tttctgaagc
      361 attgcgaacc ggccttattt ttggtgccgt cgaaaccctg acgccgctga tcggctgggg
      421 aatgggcatg ttagccagtc ggtttgtcct tgaatggaac cactggattg cgtttgtgct
      481 gctgatattt ctcggcgggc gaatgattat tgagggtttt cgtggcgcag atgatgaaga
      541 tgaagaaccg cgccgtcgac acggtttctg gttactggta accactgcga ttgccaccag
      601 cctggatgcc atggctgtgg gtgttggtct tgctttcctg caggttaaca ttatcgcgac
      661 cgcattggcc attggttgtg caaccttgat tatgtcgaca ttagggatga tggttggtcg
      721 ctttatcggc tcaattattg ggaaaaaagc ggaaattctc ggcgggctgg tactgatcgg
      781 tattggcgtc cagatcctct ggacgcactt ccacggttaa taagaacgct gccagaggtg
      841 aatattaaag tccgtctggc agtcgaacac ttcttttgct gccagtgctt gccagacttc
      901 aggcttcgca cgccaggcaa acggcgtcat ctgcaataat gcgacggctt catcaccgcg
      961 aagacgcatc agataacaca actcatcact ctgctgtaat gtaaaacctt tcagttgttc
     1021 tgcatgaggt gcatgaagat gtacttcatt gtaaatcagc cccttcagct ccattaagtg
     1081 tctcggtccc ggcgtggcag taatgaccca accgccgggc ttcactactc gcgctagttc
     1141 ttctgcttta cacggcgcgt aaatacgtat tatggcgtcc atactggtat cggaaaacgg
     1201 caaacggtgg ctggaagcaa cacaaaaagt gacctgcgga tagcgtttcg ccgccgcttt
     1261 tatcgctacc ttcgagacat ccagaccaaa cgtggtgatt tcgggcaacg catcagcaaa
     1321 tgcgtgtgtg taataccctt caccacagcc aatatccagc accgccgcgg cctttgcatc
     1381 aagccgttcc cgcagttggc cgacaattgc atcacgcagc ggctgataat gtccggcatc
     1441 taagaatgcg cggcgtgctt gcatcatttc cgcgctgtcg cccggatcac gagaccgttt
     1501 atgctgaacg ggcagcagat tgacataccc ttctttcgcc atatcaaact gatgtcgcag
     1561 gggacagata tagctgtttt tttcacgcga aagaggctga tggcaaagtg gacaggaaaa
     1621 cgacatgaca actccggcag gatactaaag gccgcaagtg taacgcgaat catgcggcgg
     1681 gcaaatatcc gaacgactgg agcgaagata aaacaaagta aaaagcctcg cattcgacga
     1741 ggctttatag cgagattgaa gcgtattcac acttcagatc agtggattcg atcagatagc
     1801 tgttacgtta acagctgccg gacctttctg accgtcctga atttcgaact caacgttctg
     1861 accttcagcc agagttttga agccattacc ctggatagcg gagaagtgta cgaacacatc
     1921 tttgctgcca tcagccggag taatgaagcc aaaaccttta gactcgttga accacttaac
     1981 ctgaccttta atctttgcca tttgaaaaat tccttagatt gttttcttcg cccgcaggca
     2041 taacatagat aaaactgaca cattactgca tgaggcacca atataaggct cggcagagaa
     2101 gcggtattca acgtcaacgt gtttactcag gacttcttta ctgaaaatgc cacacataaa
     2161 caaaactgta cctcgtttaa cccgaaatct gttatcacat acaacgttaa ttatggcaag
     2221 ccatttttaa acatgtctcg atcagacgca caaatctcga tacgtcttcc actttttttg
     2281 cacacttatg caacggaata cgcgccgata tatcattgtg cttaaccttg ccagttcagg
     2341 cagatactta aactgacgta ttttctaaca tagttcaata agttcagttg ttccaggcca
     2401 gtgggagaag ttattacata gtgcgtgcaa tatcacattt tctactatgc aatgaataaa
     2461 aagttatatc actttttctc ataaaacagt cagttaacgg ctatcaatta ccctaaagag
     2521 aagtcaatcc ccaaagggat tgtaaattta aaataagaaa aattgatgaa tgagcaaaaa
     2581 aatcaagaga gaaacgtttc gctaataaat aataccacgc ttaatcacaa atccgatgta
     2641 aacatcctaa taaattaatg agggaaatat ctttttctga tatcccgtta cgcaaattaa
     2701 taatatgata attatttgaa gtcagaccag agttttaatt tgaagcagct gcaccatgac
     2761 agtgcgtata atcagcatga attgagtaac ccttcaacga cggggttact caagattgag
     2821 gaaggattat cgttcggcga ccagacgaat aatatcttcg tcttcaaccg atttttgact
     2881 ctcaacggtc tttttatctg ctttctcagg ttcgtttgct tcgcacaaac gacgtaacag
     2941 tgcattttga cgtttttgct gatccagcaa cgcctcaagc agttcaatct gttcgttagt
     3001 ccgtgaactg gcacgattga taaaaaacca caagatgagc ccgataagaa gaaccaccac
     3061 cgatacaacc aaagacgcaa tgttcatcac gcctgaattt acaacttcgt tcatttcacc
     3121 acctctgagt agaggcgcta ttctaccact gctggagagg aagaaaatct agtgctgaaa
     3181 aaatgatatc accacgggat aaactggtta atggcacaaa ttccgctgaa aaattgtaca
     3241 tcctgatcgc acatcatgtt gaatacctgc gcccaaagca gcaagcaagc caacaccacg
     3301 acaaccagag cgacccatcg aaactttttc actccaatct ccgtttcacc taccttatgt
     3361 cattaaacta gcatgcgtta gttaaacagc gcctaactat gtcgatattc gtgctttttc
     3421 ggaatgagtc gcttgcttca gcgcatattg ccgctacgat taagcgaaca taaaaagaga
     3481 agaggttgta atgcgattca tcattcgcac agttatgctg atagcgctgg tatggatagg
     3541 attattactc agcggatatg gcgttttgat tggaagcaaa gagaatgccg cgggattagg
     3601 tttacaatgt acctatctga ccgccagagg caccagcacg gtacaatacc tgcacactaa
     3661 aagtggtttt ctggggataa cagattgccc gttgttacgt aaaagcaata ttgtggtcga
     3721 taatggttaa gggtaataaa taaccagatt ctcactcatg agtaaaatat tcttatcgca
     3781 agtgcgttta atataacgcc accttcaact ttatttttct catgctaaat aaagaacctg
     3841 tctgtttgat attcagacag gttcttactc tcatcagaac ggatagtcgt gataacccat
     3901 ttgggcagaa attttgcgcg ccgcggtatg caacattgcg acatattcct gtaaacgctc
     3961 ttcagagaaa cgcaacgtcg ggaaggagat gctcagaccg gcaatgacca cgccaaagcg
     4021 atcaaatacc ggaaccgcaa tgcaacgcag tccttcttcc tgctcttcat tatcttcgcc
     4081 atacccctgc tcgcgcacct ggtccagaac gggtaataac gcttctgtac tggtgatggt
     4141 ccgctcggta ctgcgtttat actccacgcc ctcaagaatt tgcatcactt catcgcgatc
     4201 gcgccatgcc agcagtacct taccaatcgc ggtgctgtac agcggattac gacgcccaat
     4261 ccgtgaatac atgcgcaaat tgtacattga gtcaattttg tggatgtaaa caatactgtc
     4321 ttcgtccagt gcgccgaggt ggatagtttc tttggtcagg cgggagatct cacgcatctg
     4381 gatatctgcg ctacgaatta aatcgacgtt ttgcaacgcg cgagcgccca gttcaaacaa
     4441 tttcagggtc agcgaatatt tctccgactc cccttcctgc gccacataac ctaaggtttt
     4501 catggtctgt aaaaagcgat aaacggtgct ttttgacatc atgacgcgct gcgacaactc
     4561 ggttatccct atttcgcgct cttcacccag cgcctgcaaa atgccaaaaa cttttagcac
     4621 ggaagataca gaatcaggct gtttatccag atctgcgtta gccatttatc acctcattgc
     4681 gagtgtttta taaaaatcag aactgttttt tattataatt tcgcaccagg gtggtcgcaa
     4741 tccatctttt gccggttagt tacaattctg cgacatccac cgtgaatatc agtgctagaa
     4801 tcagacccct gttgattatt caccaaagat ataaaattcc tatgccaaaa gttcaggccg
     4861 acggcctgcc attgcccctg cgatacggtg cgatattaac cattgtgatt ggtatttcga
     4921 tggctgtcct tgacggcgca atcgccaacg tcgccttgcc aacaatcgct acggaccttc
     4981 atgccacgcc ggccagttcc atctgggtag tgaacgccta tcaaatcgcc attgtcatct
     5041 ccctgctctc gttttcgttt ctgggcgata tgtttggcta tcgacgtatt tataaatgcg
     5101 gtctggtcgt ttttctgttg tcttcactgt tctgcgccct ttctgattcg ctgcaaatgc
     5161 tcacccttgc gcgtgtcata caaggtttcg gcggtgcagc gttgatgagc gttaataccg
     5221 cacttatccg cctgatctat ccacaacgtt ttctgggtag agggatgggc ataaactcgt
     5281 ttattgttgc cgtctcttct gctgccgggc cgacaattgc tgcagcaatc ctctccatcg
     5341 catcctggaa atggttattt ttaatcaacg taccgttggg tattatcgcc ctgcttctgg
     5401 cgatgcgttt tctgccaccc aatggttctc gcgccagtaa accccgtttc gacctgccca
     5461 gcgccgtgat gaacgcgtta accttcggcc tgcttatcac tgcgttgagt ggtttcgctc
     5521 aggggcaatc gctgacattg attggtgcgg aactggtggt aatggttgtc gttggtattt
     5581 tctttattcg ccgccagctt tctcttcccg taccgctgct accggtggat ttactgcgta
     5641 tcccgctgtt ttcactttct atttgcacat ctgtttgctc tttctgcgca caaatgctgg
     5701 caatggtttc cctgcccttt tacctgcaaa ccgtgctcgg gcgtagtgaa gtcgaaacag
     5761 gtttacttct gacaccgtgg ccgttagcaa cgatggtgat ggctccactg gcaggctatt
     5821 tgattgaacg cgtacatgca ggattgctgg gtgctttagg gttattcatc atggctgcgg
     5881 ggcttttttc cctggttctg ctgccagcgt cacctgcgga tatcaatatt atctggccga
     5941 tgatcttatg tggtgctgga tttggcttgt tccagtcacc caataaccac accattatta
     6001 cctccgctcc tcgcgaacgt agcggtggag ccagtggcat gttagggaca gctcgtcttc
     6061 tgggtcagag tagcggtgcg gctctggtag cgctgatgct aaatcagttt ggtgataatg
     6121 gtacgcacgt ctcgctgata gctgcggcta ttctggcagt gattgctgcc tgtgtcagtg
     6181 gtttacgtat cactcagcca cgatccaggg cataaaaaaa gcgcgtcgat caggacgcgc
     6241 tttttagtat ttacttcata ttacttcagg tattcacccg tacgcagagc ttcaatacgt
     6301 ttatccagcg gcgggtgagt catgaacaac tcactgagcg atttcgactt cccgttaatg
     6361 cagaaagcca tcatgctggt tgcttcttgc ggttcatagc tggttttcag gcgttgcagc
     6421 gcggcaatca ttttctcgcg accaaccagt tttgctgaac ccgcatcggc gtggaattca
     6481 cgatgacgcg agaaccacat ggttataatg ctcgccagaa taccaaacac cagttccaga
     6541 cccgttgcaa ccgcaaagta gatcagcggg ttgccgttgc tctcttcacc ttcatcacgg
     6601 tttccgccca taaaacccgc ggcaagctgc gccagaatac gggaaataaa gataacgaag
     6661 gtgttcacca cgccctgaat cagcgtcatg gtgaccatat caccattggc gatgtggctg
     6721 atttcgtgag caattaccgc ctcggcttca tccgggctca tgttctgcag caaaccggtg
     6781 ctgacagcaa ccagagaggc atcacggcgc gcaccggttg caaaagcatt gatgtccggc
     6841 gcatggtaga tagccacttg cggcatagcg atccccgcct gacgagcctg ggttgctaca
     6901 gtattgacca gccaacgttc cctttcgtta cgcggttgct cgatcacttc cccgccaaca
     6961 gatcgtaatg ccatccattt ggacatcaga agcgaaacga aggaaccacc aaaaccgaac
     7021 agcaaggcca tgatcatcag cccctgaacg ctgctcgact gtatccctgt caggctcagt
     7081 accagcccga aaacgaccat tacggccagg ttcgttagca ggaagagcgc gattcgcatc
     7141 ataattttct ttttacctca gtttaacaaa acgcaatatg cgatacccac atcgtatggg
     7201 ttacgcgact attttcaagt ctggatagtg cgtaagtcac cagaaagaca caactttaca
     7261 ttttgtagca tctgatttac ggcatcttgc cgctgttaaa aaatcaggca caatttcttg
     7321 tgcctgattg atattacttg acgggagcgg gttgttccgc gggtctggct ttttcaagct
     7381 tcgccagatc gagtgcgata ttcaccgtct catccagata aggatctggc tcctggtaat
     7441 ctttcggtag atcatccagt ttcttcaact ccggtttacc ttcgcgttta aagcgttcgt
     7501 tcaaacgcgc cagacgcgtc gaatcatctt cattattctc tttctcacgc acagcgtaat
     7561 tcagagaaac gatattgcgc ttgtccttca tagcgttgaa gcgcgcgata tccttcatga
     7621 tgttctggaa ctcaggatct ttcgcgatac gcgcattatg ttccttcagc agctccggtt
     7681 caaaggccgt taaatctcct gatttcacat aagtcgcggc atcaatgcta tcccacggca
     7741 gcgcgttatc ttcgaatttc tcacccgttt ccgtttcttc attacccgtc ggcatgatga
     7801 tgtctggcgt tacaccttta cgttgcgtac tgccgccgtt aacgcgatag aatttctgga
     7861 tcgtgtactg cacagaaccc agcgctggcc attcaggacg taacatctga tcgtaaatac
     7921 ggttcaatga acggtactgc tgaacggtgc ctttaccaaa tgtcggttca cccacaacca
     7981 gggcacgacc gtaatcctgc attgccgcgg caaagatttc tgaagccgaa gcactgaagc
     8041 ggtcaaccag caccaccagc gggcctttat agaagacctg tccgtcggtg tcgctgtctt
     8101 cacgaacctt gccgttgtta tcgcggacct gaacaatcgg gccggatgga atgaacagac
     8161 cggagagcga tacggcttca gtcaacgccc caccaccatt gctacgcagg tcgataatca
     8221 cactgctgac attctgtttt tccagtttct gcagttgcac tttgacatcg tctgtcaaac
     8281 ctacatagaa gcccggaata tccagcacgc cgactttctc tttaccgacg gtcttcaccg
     8341 acattttaac cgcgcggtct tcgagacgaa tacgttcacg ggtcaacgtt acagtacgcg
     8401 tcttggtccc tttaccagca ggtaaaattt ccagacgaac tttactgccc ttaggccctt
     8461 taattaaggc caccacatca tcaagacgcc agccaatcac gtcaaccatc ggcttgcctg
     8521 tttgaccaac accgacaatt ttgtcaccaa cgctgatagc tttgctcttc gctgccggac
     8581 ctcctgccac catcgaattg ataacggtgt agtcatcatc catttgcagc actgcgccaa
     8641 taccttccag cgacaaactc atttcagtgt tgaactgttc ggtattacgc ggggaaagat
     8701 agttggtatg cgggtcgatt tcacgcgcaa acgccgtcat tgccagcgag aaaacatctt
     8761 cgctgttggt ttgcgccaga cgacgaatgg caaatttgta gcggcgagtc agggtttcac
     8821 gaatttcctt atccgttttt cctgtcagct tcaggcttaa ctcgtcgaat ttgactttac
     8881 tgtcccacag cgcgttcaac tcagcctcgt ttttcggcca gggcgctttg ctgcggtcaa
     8941 ggttataagt gtcgttgccg gtgaaatcca tcggcttttc cagtaccgac aaagcgtact
     9001 ggtaacgttc aaaacggcgc ttttgcgcca gattgtagag atcgtagaaa acgtcgagtt
     9061 tacctgaacg cagttcatcg cctaactcag ttttcttttt cgcgaactgt tcaacatcgc
     9121 tttccagcag cacattgtgg ctgtaatcga gcagattcag gtagcggtca aagattttgg
     9181 ccgaaaatgc ctgatcgagg tcgaactggc gataatgaga acgggtgaag cgcgacgtta
     9241 cgcgctcact caccgtcgca tgctgcgtct cttcctttaa taccggaatt tgatcagcac
     9301 gcgtgatatc ttctacagcg aaggtctggc ctgctattgc aagcaggcca gctaacgcgg
     9361 taagcctaaa aaacatgttc atgcctggcc cggcctccgt ttcagaacac caggtgttct
     9421 gcgcgcacaa tcaaagacat acccgaattc agctggacgc ggacgccgtc tttggtgatt
     9481 tctaatacgg tggcatccat cgcgttttga cccgctttca ccttcagggc ttgtccgaca
     9541 gtcagagctg aaatgtcaga aaccggggtg tgctgttctt cgcgaggtgc ttttactgtt
     9601 tttggcgctt tctctaccgg cttttgcgaa cgaggtttac gttcagcgcc ttctttgcgg
     9661 cgtggcgtgg tcggacgtgg cttgcgttcg cggcgcggtg cgtcttcttt ctcaccagca
     9721 gctgcggcag cttcgcgttt tttcgcttgc tgttcagcac gctgtgcctg aacacgcgct
     9781 ttcgcttctt caagctgctt gcgagcatgc tcgacatgtt gctcgtccag ctcaccgcat
     9841 gggttgccgt caagatcgac acgcgttgcg ccgggtttaa caccgtaaag ataacgccag
     9901 ctcgaagtgt agagacgtaa agcggatcgc aattgcgttt tgctcaggtt catttccccg
     9961 gcaacacgat cgaccaaatc ctgaaaaata ccgattttca gcggacgcgc ttcaccttcc
    10021 gcactgaaac agtggggaaa acgttcggcc agaaacgcga ttacttcttt actgctattc
    10081 aacttaggtt gattttccat gaaatttcct gattacaacg gacgtagcca acaagcgcag
    10141 gcatgaacag gcgtcattat aatgacgcta tcagtaaatg ctacgttatc cgttgattat
    10201 cctgcgacgc tcgcaaagaa ttttttgtaa tccgtcgttg caagcacttt ttcaagctgt
    10261 gccacaagct gacgtaagcc ttgctcgtcc tcgtctgtaa agcgaccgaa gacggtacta
    10321 tcgatgtcga gaacaccaat aatctgattt ttcaccacca gtggcagaac aatttcagaa
    10381 ttactcgccg catcacaggc aatatgcccg tcaaacgcgt gaacatcctc gatacgctgc
    10441 acttgattgc gggcaaccgc agtgccgcat acaccgcgcc caacgggtat ccggacacag
    10501 gcaattttgc cctgaaatgg tccgagcacc agtgtatcgt cctcaagcaa ataaaaacct
    10561 gcccagttta cgtcagtgag acgctcatat aacaacgcac tggtgttcgc aagcgttgcc
    10621 agaaaactgg tttctcccgc catcagcgcg ttaaagtcgc gatttaaatc cgcgtaaaat
    10681 tctgctttgt tcattatata atcacttggt tgtcttacct ggaactgcca gcctattaaa
    10741 ataagcatta aatgcgttaa tgctcaagat cattcccatc atgggttaag attaatgtta
    10801 attcttatta catttggcac gtcatggctc ttaacacacc acaaattacg ccgacaaaga
    10861 agataacagt gagggctatc ggcgaggaac tgccgcgtgg tgattaccaa cgttgcccgc
    10921 aatgtgacat gctgtttagc ctgcccgaga taaattctca tcaaagtgcc tattgtccgc
    10981 gctgtcaggc aaaaattcgt gacgggcgcg actggtcgct aacgcgcctg gcggcaatgg
    11041 ccttcaccat gctgttgctt atgccgtttg cctggggcga accgctgttg catatctggc
    11101 tgttaggcat tcgtattgac gccaacgtta tgcaaggcat ctggcaaatg accaaacagg
    11161 gcgatgcgat aacgggggcg atggtctttt tctgcgttat cggtgccccc ctcattctgg
    11221 tgacctccat agcttattta tggtttggta accgattggg aatgaattta cgcccggtac
    11281 tgctgatgct tgagcggctg aaagagtggg tgatgctgga tatctacctg gtcggcattg
    11341 gcgttgcttc tataaaggta caggattatg cccatatcca ggcgggtgtc ggcttgttct
    11401 cttttgtggc gttggtaatt ttaacgacgg tgacgttgtc acatcttaat gttgaagaac
    11461 tgtgggagcg attttatcca cagcgccccg ctacccgtag ggacgagaaa ctccgtgtct
    11521 gtcttgggtg ccattttacc ggctatcctg atcagcgtgg tcgctgcccg cgttgccata
    11581 tcccgctacg cctgcgtcgc cgtcatagtc tgcaaaaatg ctgggcggcg ctgttagcgt
    11641 caatcgtttt gttgttacct gccaacctgt tgcctatctc tatcatttat ctgaatggtg
    11701 gtcggcagga agatacgatt ctttccggaa ttatgtcgct ggcaagtagc aacattgcag
    11761 ttgcgggaat cgtgtttatc gccagtattc tggtaccgtt tactaaagtg atcgtcatgt
    11821 tcactttact gttgagtatt cattttaaat gccagcaagg tttacgcaca cgcattctgt
    11881 tactgcggat ggtgacctgg attggtcgct ggtcgatgct cgacctgttt gtcatatctt
    11941 taaccatgtc gctgattaat cgcgatcaga tcctcgcttt tactatggga ccggctgcgt
    12001 tttatttcgg cgcagcggta attttgacta ttcttgctgt ggaatggctg gacagccgct
    12061 tactttggga tgcacatgag tcaggaaacg cccgcttcga cgactgaagc gcagattaaa
    12121 aataaacgcc gtatctcacc tttctggctg ctgccgttca tcgcgctaat gattgctggt
    12181 tggctgattt gggacagtta tcaggaccgg ggcaatactg tcaccatcga ctttatgtcg
    12241 gcggatggta ttgttccagg ccgtacgcct gttcgttatc agggcgttga agttggaaca
    12301 gtgcaggata tcagcctcag cgacgatctt cgtaagattg aagtcaaggt cagcatcaag
    12361 tccgatatga aagatgcgct gcgcgaagag acacagttct ggttggtgac gccaaaggcc
    12421 tcgttggcag gtgtctccgg gctggacgcc ctcgtcggtg gtaattatat cggcatgatg
    12481 ccgggtaaag gtaaagagca ggatcacttt gtcgcactcg acacccaacc gaaatatcgg
    12541 ctggacaatg gcgatctgat gatccacctg caagcccccg atctcggttc actgagcagt
    12601 ggttcattgg tctatttccg caagatcccg gtgggtaaag tctacgacta tgccatcaat
    12661 cccaacaagc aaggcgtggt gattgatgtc ctgatcgagc ggcgttttac cgatctggtg
    12721 aaaaaaggta gccgtttctg gaacgtttcc ggcgttgatg ccaacgtcag tatcagtggc
    12781 gcgaaggtga aactggaaag tctggcggca ctggttaacg gtgcgattgc cttcgattca
    12841 cctgaagagt cgaaacctgc cgaagcggaa gatacctttg gtctgtatga agacctggcc
    12901 catagccaac gtggcgtaat aataaaactg gaactgccag gcggtgccgg attaaccgcc
    12961 gactcgacgc cgttaatgta tcaggggctg gaagtcggac agctgactaa actggattta
    13021 aatcctggtg gtaaagtcac tggggaaatg accgttgatc ccagcgtcgt caccctgctt
    13081 cgtgaaaata cccgcatcga attacgtaat ccgaaattat cccttagcga tgctaatctc
    13141 agtgccctgc tgaccggcaa aaccttcgaa ctggtgcccg gcgatggcga gccacgtaaa
    13201 gagtttgttg ttgtgccagg cgaaaaagcc ctgctgcaag aaccggatgt tctgacactg
    13261 accctgacag caccagaaag ttacggtatt gatgctggtc agccgctcat tcttcacggc
    13321 gtgcaggtag gccaggttat cgatcgtaaa ctcaccagca aaggcgtcac ctttaccgtc
    13381 gccatcgagc ctcagcatcg cgaactggta aaaggcgata gcaaatttgt cgtcaacagc
    13441 cgcgtcgacg tgaaggtagg gctggatggc gttgagtttc tcggtgccag cgcctcagaa
    13501 tggatcaatg gcggaatacg tattctgccg ggcgataaag gcgagatgaa agccagctat
    13561 ccactgtatg ccaatctgga aaaagcgctg gagaacagcc ttagcgattt acccaccaca
    13621 accgtgagtt tgagtgcaga gacgctgccg gatgtgcagg caggatcggt agtgctgtac
    13681 cgtaaatttg aagttggtga agttattact gtgcgtccgc gagctaacgc gtttgatatc
    13741 gatctgcata ttaagccgga gtatcgcaac cttctgacca gcaacagcgt gttctgggca
    13801 gaaggtgggg cgaaagttca gctgaatggt agtggtctga ctgtacaggc atccccgctc
    13861 tccagagcat taaagggggc tattagcttc gataacctca gcggtgccag cgccagtcag
    13921 cgtaaaggcg acaagcgtat tctgtatgct tccgaaacag cggcccgtgc ggttggcgga
    13981 caaattacgc ttcacgcttt cgatgcggga aaactggcgg tcgggatgcc aattcgctat
    14041 ctcggtattg atatcgggca aatccagacg ctggatctga ttaccgcacg caatgaagta
    14101 caggcaaagg cggtgctcta tccggaatat gtccagacct ttgctcgcgg tggtacgcgc
    14161 ttctcagtgg tcacaccgca aatttcggca gctggcgttg agcatcttga tactatcctc
    14221 cagccgtata tcaacgtcga accaggccgg ggcaatcctc gccgcgactt tgaattacaa
    14281 gaggccacca ttactgattc gcgttacctg gatggcttaa gcattattgt tgaagcgccg
    14341 gaagccggtt cattaggtat cggtacacct gtgctgttcc gtggtctgga agtaggtacg
    14401 gttaccggaa tgacgctggg gacattgtca gatcgcgtga tgattgcgat gcgcatcagt
    14461 aaacgctatc aacacctggt gcgtaacaat tccgtcttct ggttggcatc gggttacagt
    14521 ctggactttg gtctgacggg cggcgtagtg aaaaccggca cctttaacca gtttatccgt
    14581 ggcggcatcg ccttcgccac gcctccgggt acgccactgg caccgaaagc ccaggaaggc
    14641 aagcacttcc tgttgcagga aagtgaaccg aaagagtggc gtgaatgggg aactgcgctt
    14701 cccaaataat gcccactgct ccggcgtgcc tgcgccggag cgtttatgct aaactgcgcg
    14761 cctgtttttt tgccagtggt atatgctcgt ggcccaacac accgtttatt tcccggacgc
    14821 ctttctgaca caaatgcgcg aagcgatgcc ttcgacgctc tcttttgatg attttcttgc
    14881 cgcctgtcag cgcccgttgc gccgcagcat tcgcgttaat acgctgaaaa cctccgttgc
    14941 tgatttcctg caattaactg ctccttatgg ctggacgctt acgccaattc cgtggtgtga
    15001 agaaggtttc tggattgaac gcgacagtga agatgcattg ccgttgggta gtaccgccga
    15061 gcatttaagc ggcctgtttt atattcagga agccagttca atgttgcctg ttgccgcctt
    15121 gtttgctgac ggtaatgcac cacagcgggt gatggatgtc gctgctgcac caggttccaa
    15181 aacgacgcaa attgctgcgc ggatgaataa cgaaggggca atccttgcca atgagttttc
    15241 cgccagtcgg gtaaaagtgt tacatgccaa tatcagccgc tgtggcatca gtaatgttgc
    15301 gctcacgcat tttgatggcc gcgtgtttgg tgcggcagtg ccagaaatgt tcgatgccat
    15361 tttgctggac gctccctgct ccggcgaagg cgtggtgcgt aaagatcccg atgcgctaaa
    15421 aaactggtca ccagaaagca atcaggaaat cgcagcgacc caacgggagc tgatcgacag
    15481 cgcctttcat gcattacgtc ctggcggtac gctggtttac tcgacctgta ccttaaaccg
    15541 ggaagaaaac gaagccgttt gcctgtggct gaaagagact taccacgacg cagtagagtt
    15601 tttaccgctt ggcgatctct tccctggtgc aaataaggcg ctgaccgaag acggcttttt
    15661 gcatgttttc ccacaaattt acgactgcga aggcttcttc gttgctcgtc tgcgtaaaac
    15721 tcaggcgatc cccgtcttac ccgccccaaa atacaaagtg ggtaatttcc cgtttagccc
    15781 ggtgaaagat cgcgaagccg gtcaaattcg tcaggcggct gcaagtgttg gcttaaactg
    15841 ggatgaaaac ctgcgactct ggcagcgtga caaagaactg tggttgttcc cggtaggcat
    15901 tgaagccctg atcggtaaag tccgattttc tcgcttgggg attaaacttg ccgaaacgca
    15961 caacaaaggt tatcgctggc agcatgaagc cgttattgcc cttgcctccc ccgacaatgt
    16021 gaacgctttt gaactgacac cgcaggaagc ggaagagtgg tatcgcgggc gcgatgttta
    16081 cccgcaagcc gcgccagtgg cggatgatgt attggttact ttccagcatc agccgattgg
    16141 tttagccaaa cggattggtt cacgactgaa aaacagctac ccgcgtgaac tggtgcgcga
    16201 cgggaaactt tttaccggta acgcctgaca gcgcacaaaa aaagcgcact ttttgactgg
    16261 cacattcgac tgcctcaact aggctgaaaa atggtgcgat cggactggtc gtaccacaac
    16321 cggcagctaa atggagagca cgaagatgaa aaccagtgtg cgcataggcg cttttgaaat
    16381 cgacgacggc gaattacacg gcgaatcgcc gggtgatcga acgttaacca ttccttgtaa
    16441 atcagacccc gatttatgta tgcaactgga tgcctgggat gctgaaacca gtatccctgc
    16501 cctgctaaat ggcgaacact ctgtccttta ccgtacccgt tacgatcaac aatctgatgc
    16561 ctggattatg cgtcttgcct gatccaaaaa gaacccgtcg gcatggcggg ttatttgtcc
    16621 gggttattcc cccgttgtaa aatctctcct aaacttaacg gtacggcacc acacttcggg
    16681 gatgaaatgt tcgcgctggt actttttgtt tgctacctgg atggcggttg tgaagatatt
    16741 gttgtggatg tctacaacac ggaacagcag tgtctttatt ctatgagcga tcaacggatc
    16801 cgccatggcg gttgttttcc gattgaggat tttatagatg gtttctggcg acctgcacag
    16861 gagtacggtg atttttaatt attgcaattg cacaagagtc agttctcccc caaagacagc
    16921 accggtatca atataatgca ggttgccaat atccacgcga tggcgcaaag gtgtatgacc
    16981 aaaccagaaa tgatcagcac ctgcaattcc ctgccctttt tggcgttcac ctaatcgcga
    17041 gcggctccac aagacctgat gcaaatcgac gtccttttgc cattcataga catcatctgg
    17101 ataatcggca tgagcaataa catgtttgcc ggtgcgactg tgtaattcaa gaataaaggg
    17161 caaatgctga catttttcca gcgccgtttt cgcttgtttc tgttgattat ctgccagcgc
    17221 aataaaccag tcgccgccat ttatcaacca taaagacatc tgccgggatg ccagcgcatc
    17281 catcgccatc tgttcatgat tgcctcttac cgcacgaacc caatgttgtt ccagtaactg
    17341 caggcaacgt aaactttgcg gcccacgatc gataacgtct cccactgaga taagtaaatc
    17401 tcgccacgga tcaaaacgac aatgccataa tttgcggcgc aactgctcaa gacaaccgtg
    17461 tatatcgcct gaaagccaga tatgtcgcca ttgatgaccc gcaattctct gataaacggg
    17521 cgcaggctgt ttcatcaata ttttcctccc gcgctaaaga tcacataatc ttaacaagaa
    17581 tgttaaaaaa cgctggactc agacagtaga gtgtgtgtta tggttgacta taaagtcagc
    17641 gaagggaatg cttctggctt ttaacagata aaaagagacc gaacacgatt cctgtattcg
    17701 gtccagggaa atggctcttg ggagagagcc gtgcgctaaa agttggcatt aatgcaggct
    17761 tagttgcctt gctctttaag aatagatgac gacgccaggt tttccagttt gcgtgcaaaa
    17821 tggtcaataa aaagcgtggt ggtcatcagc ttaaatgtta aaaaccgccc gttctggtga
    17881 aagaactgag gcggtttttt attggaaatc aaaaggctat tttaggtaat taacagagtt
    17941 tttcagctcg ctctataaac ggtgccagac tcattttttc gccgggattg ttaggatcat
    18001 caatctgaat caccgaaatg ggttgggctt tagtcttccc actggcaact tccttttgtg
    18061 cgatatcgtt taaaggatac tgcacgagag tacttgggtt aatgacatac aaagcattac
    18121 ctggtcggca agtcagcatc acctcttcgc gattaaacgc ccatttttct ttacccactt
    18181 caaaacggct gacggtaatc acctgcggtg ccgccagcgc agctgcagaa ctggtgagta
    18241 acagaaacgc cagaatactg tttttcatca ttttcgtaat ccatcaaaaa ggttcgagag
    18301 tcgcaagcag gctgatgatc accagcacta ccgcacctat cgcccattcg agtttagtca
    18361 tccagacaaa ccagggagcc gcacgatctt catcctgcct catgcgcggt acaagaacat
    18421 aacgattagc cagcgcgatg accaccataa tcatcaccag gatggctttg agcaataaga
    18481 gttggcccca gtaagtcgta agcgtggggg gaaatccggt aatcaacaat gcattaagca
    18541 cgccgcttgc cagtacgccg atcaccgcaa aatgcccgca ccaggaaaaa cgcatcagcg
    18601 cctgaatagc ctggtgtcgc caacggcctt tgatgagttg catacaccag agcaccggca
    18661 gtaatccccc aaaccacgcc gccgcacaga tcaggtgaat tgcgtgatta gtctgatgga
    18721 ttttcgccgt taccccttca ttcaacgtcg catgcccgac tcccgccagc agaataaatt
    18781 gcgcggtagt gagcataaac agcaaacgtg gcatattccg cggttgcata agggcgacga
    18841 tcaacgttac gagggcgaga acaatttgcc ataaccagac gccaccaaac tgcgtttgca
    18901 aaacagccaa ccaaatgttt ggcgaaaata catctgacca tcccgctccc atcaacccgc
    18961 cctgaacggc gagcattgca gtggcactga tcaaactcca gacggcagcg tgctgttgta
    19021 aacgcagaaa acgcttcgtc agcaagcgac gaatcgttaa gggggccagc caggcaccgt
    19081 acattgcgaa gccaaaaacc agcatcagcg aggtgaaatg gataaatcgc agcgcgatcc
    19141 aggtaaacgc cagcatgatt tatttcacac taaaggtgta atgtcctttc gttttatgcc
    19201 catccaccga aacaacatgc cagtcaacgg tgtaggttcc gggtttcagc gaatcggcca
    19261 gtggaacaat cagttgcttt tggtcctgct cattccgctt cgccggtaat gttttaatat
    19321 tttcgttttt tggccccgtg atttttgcac cactgaatcc tgtttcaacg ccttccgaga
    19381 agtttaaagt gattgcctgc ggagctgctg tcacttgtgc atttgccgca ggatactgat
    19441 gcgttaaatg cgcatgtgcc cagacggaag gtgtcaccaa cgaagtggtc aggattgcca
    19501 gcgcgtagcg aagggagcgt gcagttgaag ccatattatc tattcctttt tgtaataact
    19561 tttttacaga gcataacctt gtctaatgtc tgagtcgagg atcatcaatt ccggcttgcc
    19621 atcaaggctc actcttagta acttttgccc gcgaatgatg aggagattaa gaatgctgaa
    19681 gaatctggct aaactggatc aaacagaaat ggataaagtg aatgtcgatt tggcggcggc
    19741 aggggtggca tttaaagaac gctacaatat gccggtgatc gctgaagcgg ttgaacgtga
    19801 acagcctgaa catttgcgca gctggtttcg cgagcggctt atcgcccacc gtttggcttc
    19861 ggtcaatctg tcacgtttac cttacgagcc caaacttaaa taaaacttat acatagttac
    19921 actttcttac ataacgcctg ctaaattatg agtattttct aaaccgcacc cataatttgc
    19981 agtcattttg aaaaggaagt cattatgtcg ttctggaaag ttgcagcagc gcaatatgag
    20041 ccccgtaaaa cctcactcac cgaacaggta gctcatcatc tggagtttgt gagggccgcc
    20101 gccaggcaac aatgtcagct gttagttttt ccatcgcttt ctttactggg gtgtgattat
    20161 tcgcgacgtg cccttcctgc cccacccgat ctctcgctat tagacccgct ttgttatgcc
    20221 gcaacgacct ggcgaatgac catcattgcc ggccttcccg ttgaatataa cgatcgcttt
    20281 attcgtggaa ttgcggtatt cgcaccgtgg cgaaaaacgc cagggatcta ccatcagagt
    20341 tatggtgcat gtctgggccg tcgttccaga accatcacgg tggtcgatga acaaccgcaa
    20401 ggtatggata tggacccgac ctgttcactg tttacgacag ggcaatgtct gggagaaccc
    20461 gacctgctgg cgtcggcccg tcgcttacaa tttttttcac atcagtactc gattgcagtg
    20521 ctgatggcaa atgcccgtgg taacagtgca ctatgggatg aacatggtcg tctcatcgtt
    20581 cgcgccgatc gtggttcatt attgttagtc ggtcagcgtt catcacaggg ttggcaaggc
    20641 gatatcattc cattacgcta ggcttttcgg cctggagcat gccatgttgc gcattatcga
    20701 tacagaaacc tgcggtttgc agggagggat cgtagagatt gcctctgttg atgtcattga
    20761 cggaaaaatc gtcaatccca tgagccacct ggtgcgtccc gatcgtccta ttagtccaca
    20821 ggcgatggcg attcatcgca tcaccgaagc catggtcgcc gataaaccgt ggattgaaga
    20881 tgtgatcccg cactattacg gtagtgaatg gtatgtcgcg cataacgcca gctttgaccg
    20941 ccgcgtactg cctgaaatgc ccggtgagtg gatttgcact atgaaactgg cccgtcgttt
    21001 gtggccaggg atcaagtaca gcaatatggc gctatataaa acacggaagc tcaatgtaca
    21061 gacgccgccg ggcctgcatc atcaccgcgc gttgtatgac tgttatatca ccgccgcgtt
    21121 gcttatcgat attatgaaca cctctggctg gacggcagaa cagatggccg atatcaccgg
    21181 acgtccatcg ttgatgacga ccttcacctt tggcaaatac cgtggcaaag cggtttccga
    21241 cgttgccgaa cgcgatccgg gctatctgcg ctggttgttt aataacctcg acagcatgag
    21301 cccggagctg cgtttaacac tgaaacatta tctggaaaat acttaatccg ctgactgagc
    21361 gggtaatgtt ccctgcgcca gcgcgaccag aaaagcatat tccatcgcta cgccttcgta
    21421 cgatttgaag cgaccagatt taccgccatg acctgagtcc atgtcggtac agagcaataa
    21481 aagatggtca tcggttttca gctcgcgcaa tttagcgacc cattttgccg gttcccaata
    21541 ttgcacctga gaatcgtgta aaccggtcgt taccagtaaa tgcggataag cctgtgcggt
    21601 gacgttgtca tacgggctgt agcttttcat atactcgtaa tattgcggat cctgcggatt
    21661 cccccactct tcaaattcgc cggtggtaag aggaattgat tcatccagca ttgttgttac
    21721 aacatcaaca aacggtacct gggcgataac gccgtggaac aattccgggc gttgattaat
    21781 cgcaacgccc atcaacatac cccccgcact cccgcccatc gcataacaga gcgaaggaga
    21841 gccatagccc agttttaaca atgcatcgca ggcatcaaga taatcattaa acgtattttt
    21901 cttcttcaga aatttaccgt cctcgtacca ttgttgcccc agctcaccgc cgccacgaac
    21961 atggacaatg gcgtagacaa atccacgatc caacaaactc aagcggctaa aactgaaatc
    22021 ggcatcaata cttgcgccgt aagaaccgta gccatacacc agcagcgggt tgtgtccttt
    22081 gcgaaaatgt ttgcgatgat agaccagcga aaccggaact tcgacgccat cacgggcgac
    22141 tatccacagg tgttcactgc ggtaattcgc cgcatcaaaa ccaggaactt ccgtttgttt
    22201 taatacacga cgctcaccgg tatccatatc cagttcaaac aaagtatctg gtgtggtcat
    22261 ggacgaataa ccataacgca atcgcgcggt ttcaggttct ggattgtagg caatccaggt
    22321 cacataggcc ggatcatcaa aggcaatgcc aatgacttcc cgggtcttgc ggttaatttg
    22381 ccgcaaactg gttaacccgc gctgacgctc ttcaaccacc agccagtcgg taaacagcgt
    22441 aaacccttcc agcataatgt tatcacgtgg cggaattaac tcttcccact gttgctcatc
    22501 acgcatacgg gtacggtata agccaaagtt tttaccgtgg cggttggaac gcagataaaa
    22561 ccgatgctgg tagtgatcaa ggctgtattc gtgatctttg cggcgaggca gaaaaacaaa
    22621 cggctcggca tcggccattt ccgcgtccag caggcgaact tcactggtgg tggcgctggc
    22681 cagatgaatg accacataat gcttcgaggt cgttttatgc aggctgacgt aataggtatc
    22741 gtctttttct tcgtaaatca gtttatcttg cgatgctggc gtaccgatgg cgtgacgcca
    22801 gacctgataa ggcagcagcg tcactggatg cttgcgaaca tagtagaaag tccaggagtc
    22861 atttgcccag acaaagctgg gttcaacgtt atccagcagt tccgggtacc agttacctgt
    22921 ttccagatta cgaaaacgaa tgccgtactg ccgtcgggaa agaaaatctt ctgccagtgc
    22981 cataatggtg ttatctggcg taatcgccat tccgcccatc gaataaaact cactatgagc
    23041 cgcgcgcttg ttggcatcga gcaatgtttc ccactcgtcc cactcttcac tgaacgccga
    23101 ttgacgctgg tagatagcat attcacagcc tggttcataa atatgccgat agcggtagcc
    23161 atttttgatg tagggcgcag aaacttctcg ttgcggaatg cggtcgatga tttcctttaa
    23221 gatgcgatcc tgcaaggctt gttgcgaggc catcacccga tgaccgtaac tattttcttg
    23281 ttgcagatag tccaggactt ccggctgaga acgcgtatcg tcccgcagcc agtagtaatt
    23341 atcgatgcgc gtatcgccat gaagcgtcat ggcgtgggga atgcgggcgg cttttggtag
    23401 catgttattg ttctttctgg ttgaaacatc ttataagggt ggcaaaactc accgtggatg
    23461 caagcgaaac agggtagtca ttgcttagat gatgacaggt aatggcgcgg atatcgactg
    23521 ttatgcagac agagaaaatc agcctgttca aatggctgtg cggttctgga taacccgaaa
    23581 taatcagctt caggctatcc agagagcgga attactccgc caaagtacgt ttttgctgtt
    23641 cgagatcgcg ttcaatgcct tcacgaacat cctgggggat tttcagcgca tcacccagtg
    23701 cattcaggta actgcgttcc ataaaatggt caatatcaat agccgcgcag ctcaggaaat
    23761 agatttccag cgcctcttct tcattgcgga ccccggtagc caggcgttgt ggatccagcg
    23821 gttgttcgat tgcctgctca atgagtacac gccccttctc ttccacaccg gcttctcgca
    23881 attgctggtc gattgccgca cgttctttgg catcaatatg gccatcactt ttagcggcaa
    23941 agaccagcgc aaggatcaaa cgttccgtac gctcatccag cggcgtactt tgcgcgccaa
    24001 actgcggttc gtcctgatgc gccgcgcgaa ttttatcttt gtatttattc cacagcaccg
    24061 tacccgctac cgctccgccg ccaaccagta acgcgtttgt gccatatttc gtaagaagtt
    24121 tacgtgctga tttatttgcg accagcagcc cagccagtcc gtctaatgcg ccaggcacta
    24181 aaagtttgcc caatccctga tccgcagacg aggaggtaga agaacggctt tgcccaagca
    24241 gggattgcag ttgatttaac cagttagcca tatttgctcc tcaataacca tttttagtgc
    24301 ctgtagcata gcggatgagg atgtcagaaa atgtatatcg agacaaaaga tgcgcaaata
    24361 agcggggcgg taggccgtta ttcaaagaaa ttcgcgccag gtaaggtgtt cactgtatat
    24421 aaggtgataa gggtatttta ggtgaaagta gtttgacatt ttgagtggaa ctgtttgatg
    24481 ctgattgccc ggttcgacgc tactgcgtct tctcaggcct acgattgtgt tgcgttgtag
    24541 ggcggacaag gcacccgcgc cgcatccgcc aacggtcaat cgaattatca gaagccatta
    24601 acgccgctga tattccgcca ttcccgcttt gcagtccacg ctgacctggt aatgaatatc
    24661 ggcactttta ccacgcacgg ttagcggtac tgaccattta tcatctttcc cctgaatgtc
    24721 ctgcaaactg acccatgcca cgggatcggc ctgaccgaca attttttgat catcagccca
    24781 gcgcgccacg cgattttgtt ggtaatcacg ttttacgctc gcggcaattc cggcagcatc
    24841 cagaccttca cactttggga aagtgaccga cttgctggtt tcattattgg cagcgaaaac
    24901 tgatgcgcag gcagaaacca acaacagccc taaaaacgcc cctctttttt tcatgttttt
    24961 ctccatagca caatgattca ggagaaagca tggtacaaat tgtcaggagc gcaagttgct
    25021 tcaggccgcg tgtgaggcat cttcctcttc ggattcagca ccgacgacct gtggggacgg
    25081 taatttgccg gttttcagaa tggtgctcag cacatctttt tgttctgcca gccatagcga
    25141 aagggcttca cgttgctcat cttccatttg cactggagaa tttgtcagcc aggtgtcaag
    25201 cagatcggcc gtatcaagca tcttgtcata agcatcggct tcctttttgc tggtaaacga
    25261 cattttctct tcgccctcac gaatgactac gtatttaact tcaaccgcca tttgcagcct
    25321 ctcataataa ctgtaatttt atacagtata tttcttttca gttgggaaat caacatcagc
    25381 aataaagaca cacgcaaacg ttttcgttta tactgcgcgc ggaattaatc aggggatatt
    25441 cgttatgacg ttattaggca ctgcgctgcg tccggcagca actcgcgtga tgttattagg
    25501 ctccggtgaa ctgggtaaag aagtggcaat cgagtgtcag cgtctcggcg tagaggtgat
    25561 tgccgtcgat cgctatgccg acgcaccagc catgcatgtc gcgcatcgct cccatgtcat
    25621 taatatgctt gatggtgatg cattacgccg tgtggttgaa ctggaaaaac cacattatat
    25681 cgtgccggag atcgaagcta ttgccaccga tatgctgatc caacttgaag aggaaggact
    25741 gaatgttgtc ccctgcgctc gcgcaacgaa attaacgatg aatcgcgagg gtatccgtcg
    25801 cctggcggca gaagagctgc agctgcccac ttccacttat cgttttgccg atagcgaaaa
    25861 ccttttccgc gaagcggttg ccgccattgg ctacccctgt attgttaaac cggtgatgag
    25921 ttcttccggt aaagggcaga cgtttattcg ttctgcagag caacttgctc atgcatggga
    25981 gtacgctcag caaggtggtc gcgccggagc gggccgcgta attgttgaag gcgtcgttaa
    26041 gtttgacttc gaaattaccc tgctaaccgt cagcgcggtg gatggcgtcc atttctgtgc
    26101 accggtaggt catcgccagg aagatggcga ctatcgtgaa tcctggcaac cacagcaaat
    26161 gagcccgctt gcccttgaac gtgcgcagga gattgcccgt aaagtggtgc tggcactggg
    26221 cggttatggg ctgtttggtg tcgagctgtt tgtctgtggt gatgaggtga ttttcagtga
    26281 agtctcccct cgcccgcatg acaccgggat ggtgacgcta atttctcagg atctctcgga
    26341 gtttgccctg catgtacgtg ccttcctcgg acttccggtt ggcgggatcc gccagtatgg
    26401 tcctgctgct tctgccgtta tcctgccgca actgaccagt cagaatgtca cgtttgataa
    26461 tgtgcagaat gccgtaggcg cagatttgca gattcgttta tttggtaagc cggaaattga
    26521 tggcagccgt cgtctggggg tagcactggc tactgcagag agtgttgttg acgccattga
    26581 acgcgcgaag cacgccgccg gacaggtaaa agtacagggt taaacccggc aaaaaaacgc
    26641 tacaaaaatg cccgatcctc gatcgggcat tttgactttt acagcttagc gccttctaca
    26701 gcttcacgcg ccagcttagt aatgcggtcg taatcgcccg cttccagcgc atctgccgga
    26761 accagccagg aaccaccgat gcacagcacg cttttcagcg ccaggtagtc acggtagtta
    26821 gccggagaaa taccacctgt cgggcagaaa cggacctggg agaacggacc cgcgatcgcc
    26881 tgcagggctt tcacaccgcc gttagcttca gccgggaaga atttgaactc tttcaaacca
    26941 tagtccatac ccagcatcag ttcggaaaca gtgctgatcc ccggaatcag aggaatagtc
    27001 ccttcggtag cagctttcag cagcggctcg gtcagacccg ggctaattgc aaactgtgca
    27061 cctgcttcag tgacttctgc cagctgctgt ggattcagca ccgtaccggc acccacaatc
    27121 gcttcaggca cttctttggc gatagcacgg atagcgtcaa ctgcgcactc ggtacgcaga
    27181 gtcacttcca gaacgcgcac cccaccagca accaacgctt ttgccatcgg caccgcgtgt
    27241 tccagttttt ttaccacgat aaccggtaca accgggccgg tggtcaggat tgattctgca
    27301 cttgttttcc agtttttcat cagagttttc tctcgcctga ttacaaattt gtcgtcttaa
    27361 aaagtgatac aggttgcgcc ctgttcggca ccggacagtt tttcacgcaa ggcgctgaat
    27421 aattcacgtc ctgttcccac gcgtgacgcg ctcaggtcag gaatgtgcgg ttcgcgagca
    27481 gccagttccg cttcgtctac cagcagcgtc agttcgcctg tctgtccatt cacacgaatg
    27541 atgtccccgt cgcgcacttt tgccagcagc ccgccatcgt aggcctctgg tgttacgtgg
    27601 atagctgacg gcactttacc tgaagcgccg gagagtcgcc catcggtaac taacgcaatt
    27661 ttgaaacacc ggtccaataa tacaccaagt ggcggcatga gtttatgtaa ttctggcatt
    27721 ccgttcgctt ttggcccctg atgacggaca acaacgacac aatcgcggtc cagtaaaccc
    27781 gcttcaaagg ccggcataac gtcatgctgg ctttcaaaaa caaccgctgg cgcttcaatc
    27841 acctggttct ctaccggcac ggcagaggtt ttcataaccg ctcgccccag gttaccgctt
    27901 aacacttttg tcccaccatg atgagagaaa ggttgttcga aggacgcgat cacattgttg
    27961 tcgagtgatt tttctgcccc ttcccgccag tccagttcac cattattcag ccatggttca
    28021 agggtataac gagacagacc aaagcctgcc accgtattga catcttcatg cagcaggcct
    28081 gctttgagca gttcacgtac cagaaccggt acgccacctg ccgcctggaa gtggttaata
    28141 tcggccggac cgttcgggta gagacgtgcc atcagcggta caacatcaga aaggtcagag
    28201 aagtcatccc agttaatctg aataccggct gcgcgcgcca tcgccaccag gtgcatggtg
    28261 tggttagtgg aaccaccggt cgccagcagt gcaacgatac cgttcaccac cactttctca
    28321 tcgatcatct taccgatcgg catccattca ttaccattac cggtcatgcg cgtaacctgg
    28381 cgcgcagctg cagcggtcaa agcatcgcgc agcggagaat ccggatgaac aaaagaagag
    28441 cctggcaact gcatccccat aaactccacc accatctggt tggtgttggc agtaccgtag
    28501 aaagtacatg ttcccggcgc atgataagac gcggcttctg actccagtag tgccatgcgg
    28561 tccactttac cttcggcata aagctggcga atacgcactt tttctttatt tggcaaaccg
    28621 cttgccatcg gtccagacgg cacaaacacc gcaggcaaat gaccaaacga cagggctgcc
    28681 atggtcagac ctgggacaat cttgtcgcac acaccgagaa acagagcacc atcaaacatg
    28741 ttatgggaca gccccaccgc cgcagacatc gcaatcactt cgcggcttaa cagcgacaat
    28801 tccattccat cctgcccctg ggtgacacca tcacacatcg ccggaacacc gcccgcaacc
    28861 tgaccaaccg cattcgcttc atgcagggct ttacgaatga tttccggata gtgttcataa
    28921 ggctggtgag cggagagcat gtcgttatag gaggtgatga tggcgatatt gttacgcaac
    28981 atgcttttca aagaggcttt gtcttctggc tggcaggcag cgaaaccgtg tgccaggtta
    29041 ccgcatgcca actgcgaacg atgaacggtc gaagttttcg cttgttctat ccgggcgagg
    29101 taagcagagc gagtctcgcg cgaacgttca atgattcgat ttgttacgcg taacaattgt
    29161 ggattcataa aggctcctga aattgagttg tcagagcagg atgattcaca acacgtttca
    29221 ttcagaggat tcatgactga aacgcctgta accggagctc atagggcaaa aacgtttcag
    29281 tcagtgtaat aaaaaaagcc tcgtgggtga atccgcacga ggcctgaaag tgtaaaaatt
    29341 gttctacaat ctgtgcaaga tcatgttacc ggtaaaataa ccataaagga taagcgcaga
    29401 tattactcaa actcattcca ggaacgaccg tcacgggtaa tcatcgccac cgaggcaacg
    29461 gggccccagg ttccggcctg atacggtttc ggcgcatcat tgtccatcgc ccacgcctca
    29521 gtaatggagt ctacccattt ccaggcttct tccacttcat cacgacgtac aaacagtgcc
    29581 tgaataccac gcatggtttc cagcagcaga cgttcatagg catctgccag atgcgtttga
    29641 ttaaaagttt ctgaatagct cagatccagc ttggtgattt gcaggttatg tttgtggtca
    29701 aggccaggaa ctttattcag cacctggata tccacgcctt catcaggttg cagacggata
    29761 gtcagtttat tctgcggcag atcctgccac gactctttaa acagattcag ttcaggtgtt
    29821 ttgaaataga ccacgacttc agaacatttg gtcggcagac gtttaccagt acgcaggtag
    29881 aatggcacac cggcccagcg ccagttatca atgtcgacgc ggatcgccac gaaggtttct
    29941 gtattgctgc tcttgttcgc gccctcttct tccagatagc ccggcacttt tttgccctga
    30001 gcgaagcccg cagtatattg cccgcgtacg gttttttcgc gcacgttgga gcggtcgatg
    30061 cggcgcagag acttcagtac tttcactttt tcatcgcgga tgctgtctgc gctcaggtca
    30121 gacggcggag acatcgcaat catgcaaaga atttgcagca ggtggttctg gatcatgtcg
    30181 cgcatctgac cggctttatc aaaatagccc cagcgccctt cgatccccac ttcttctgcc
    30241 acggtaatct caacatgatc aatggtgcga ttgtcccagt tgttcacaaa cagagagtta
    30301 gcaaaacgca gcgccaacag gttcagcact gtttctttac caagatagtg gtcgatacgg
    30361 taaacctggc actcctcgaa gtattcgcca acctgatcgt tgatttcctg cgaggtcgcc
    30421 agcgacgtcc ccagcggttt ttccatgact acgcgtgccg gtttagcatt cagttttgcc
    30481 tcaccaagcc ctttgcaaat tgcgccaaaa gtgctgggcg gcatggcaaa gtagttaatg
    30541 gtgatacgat ttttttgatc cagcatcgcg ccgagacggc tgaatgcagc agtgtcattg
    30601 acatcaagat tacaaaaatc cagacgtgca ctcagggtgt cccataaacc ttcatcaatg
    30661 gtttctttca tgaaagtttc gagcgcctcg cggacaactt tggtataagc cgctttatcc
    30721 cagtcagcac gccctacgcc gataatccgg gtgtccgggt tgagctgacc ggctttttcc
    30781 agttgataca gggaaggcag caatttacga cgcgcaaggt cgcctttcgc gccgaaaatg
    30841 accaggtcac aggcctgggc tgtttgcgtt accgccatgt cattctcctt aagttaacta
    30901 acccggtact taagccaggg tatacttgta attttcttac ggtgcactgt actgctttta
    30961 cgagcttgcg aaaactgtaa acacttatcc acccgtgcga ttacggtaaa agcgcgcaaa
    31021 gtgtggcaaa actgataaaa aaatcatcgt tttccattgg gtaaaaatct gacactgatc
    31081 atgttatgaa aaaaaataac aactttttta tctgtttttg tcattaacac agcacacagg
    31141 cgtaatatct gacaaaactg catttcgatt tctttcagag cggaaatcgt cattacccgt
    31201 gagtctcttt acatcatgaa tatgctggaa aaaatccagt ctcagctgga acatttgagc
    31261 aaatctgagc gcaaagttgc cgaggtcatt ctggcttcgc ccgataacgc gatccattcg
    31321 agtattgctg ctctggcgct ggaagccaat gttagcgaac cgacggtgaa tcgtttctgt
    31381 cgcagcatgg acacacgcgg ttttcctgat tttaaacttc atctggcaca aagtctggcg
    31441 aatggcactc cctatgttaa tcgcaacgtc aatgaagatg acagcgttga atcatacacg
    31501 gggaaaatat ttgagtccgc aatggcaacg ctcgatcatg tccgtcattc actggataaa
    31561 tctgccatca accgcgccgt cgacttgctc actcaggcaa aaaaaatcgc ctttttcgga
    31621 ttaggttctt cagccgccgt tgcccacgat gcgatgaata agttctttcg ttttaatgtt
    31681 ccggtggtgt actccgatga tatcgtgctg caacgcatga gttgtatgaa ttgtagcgac
    31741 ggagacgtgg tggtgctgat ttctcatact ggaagaacaa aaaatctggt cgagctggcg
    31801 cagctggcac gcgaaaacga cgctatggta attgccctca cctctgcggg taccccgctc
    31861 gcccgggaag caacgctggc aattaccctc gacgtaccgg aagatactga catttatatg
    31921 cccatggttt ctcgacttgc acagctgacc gtgatagatg tgctggcgac aggatttact
    31981 ttgcggcgcg gtacaaaatt cagagataac ttgaagcggg tcaaggaagc gctgaaggaa
    32041 tcgcgttttg ataagcagtt acttaattta agtgacgatc gctaaaaacg attgtcactg
    32101 tcctaatctt atacgacatc cgaatgagat taatttatcg ccatcgcggc gttatttcat
    32161 tcggatttca tgttcaagca acacctggtt gtttcagtca acggagtatt acatgtccag
    32221 aaggcttcgc agaactaaaa tcgttaccac gttaggcccg gcaacagatc gcgataataa
    32281 ccttgaaaaa gttatcgcgg cgggtgccaa cgttgtacgt atgaattttt ctcacggctc
    32341 gcctgaagat cacaaaatgc gcgcggataa agttcgtgag attgccgcaa aactggggcg
    32401 tcatgtggct attctgggtg acctccaggg gcccaaaatc cgtgtatcca cctttaaaga
    32461 aggcaaagtt ttcctcaata ttggggataa attcctgctc gacgccaacc tgggtaaagg
    32521 tgaaggcgac aaagaaaaag tcggtatcga ctacaaaggc ctgcctgctg acgttgtgcc
    32581 tggtgacatc ctgctgctgg acgatggtcg cgtccagtta aaagtactgg aagtccaggg
    32641 catgaaagtg ttcaccgaag tgaccgtcgg tggtcccctc tccaacaata aaggtatcaa
    32701 caaacttggc ggcggtttgt cagctgaagc gctgaccgaa aaagacaaag cagacattaa
    32761 gactgcggcg ttgattggcg tagattacct ggctgtctcc ttcccacgct gcggcgaaga
    32821 tctgaactat gcccgtcgtc tggcacgcga tgcaggatgt gatgcgaaaa ttgttgccaa
    32881 ggttgaacgt gcggaagccg tttgcagcca ggaggcaatg gatgacatca tcctcgcctc
    32941 tgacgtggta atggttgctc gcggcgacct cggtgtggaa attggcgatc cggaactggt
    33001 cggcattcag aaagcgttga tccgtcgtgc gcgtcagctt aaccgcgcag taatcactgc
    33061 gacccagatg atggagtcaa tgattactaa cccgatgccg acgcgtgcag aagtcatgga
    33121 cgtagcaaac gccgttctgg atggtactga cgctgtgatg ctgtcggcag aaactgctgc
    33181 tggtcaatac ccgtcagaaa ctgttgcagc catggcgcgc gtttgcctgg gggcggaaaa
    33241 aatcccgagc atcaacgttt ctaaacaccg tctggatgtt cagttcgaca atgtggaaga
    33301 agccattgcc atgtcagcaa tgtacgcggc taaccacctg aaaggcgtta cggcgatcat
    33361 caccatgacc gaatcgggtc gtaccgcgct gatgacctcc cgtatcagct ctggtctgcc
    33421 aattttcgcc atgtcacgcc atgaacgtac gctgaacctg acagctctct atcgcggcgt
    33481 tacgccggtg cactttgata gcgctaatga cggtgtagca gctgccagcg aagcggttaa
    33541 tctgctgcgc gataaaggtt acttgatgtc tggtgacctg gtgattgtca cccagggcga
    33601 cgtgatgagt accgtgggtt ctactaatac cacgcgtatt ttaacggtag agtaagtacg
    33661 ttgccggatg cggcgaaaac gcctcatccg gcctacagtt caatgataat tcaacagatt
    33721 tcgaatcttc tgaagcaaac ttgaacttat catcagtcga aggcctctcc tcgcgagagg
    33781 cattttttat ttgatgggat aaagatcttt gcgcttatac ggttggattt cgcccggttt
    33841 gcgagttttc agcaatttta atatccaggt gtattgttct ggtcgcggac caacaaaaat
    33901 ctcgacttct tcattcatcc gccgcgcaat cgtatgatca tccgcctcta acagatcatc
    33961 catcggtggg cgcacctgaa tcgtcagacg atgcgtcttg ccatcataaa tcggaaacag
    34021 cggtacaacg cgcgcacggc acactttcat caaacgacca atcgcgggca acgtcgcttt
    34081 ataggtggca aagaaatcaa caaattcgct gtgttctggg ccatgatcct gatcgggtaa
    34141 ataatatccc cagtaaccct gacgtaccga ctggatgaat ggtttaatac catcatttct
    34201 cgcatgcaga cgaccaccaa agcgacggcg caccgtgttc cagacataat caaaaaccgg
    34261 gttgccctga ttatggaaca tcgctgccat tttctgccct tgcgaggcca tcagcatggc
    34321 aggaatatcg acggcccaac cgtgcggcac cagaaaaatc actttctcgt tattacgccg
    34381 catctcttcg atgatctcca gcccttgcca gtcaacgcgc ggctgaattt tctccggccc
    34441 gcgtattgcc aactcagcca tcattaccat cgcttgcggc gcggtggcaa acatctcatc
    34501 tacaatcgct tcgcgttcag cttcactacg ttctggaaag cagagcgaca gattgattaa
    34561 cgcacgacgg cgtgagcttt ttcccagtcg tccggcaaaa cgtcccagcc gtgccagaat
    34621 gggatcacgg aactttggcg gcgttaaagc gatacccgcc atcgctgcta cgcccagcca
    34681 tgctccccag tagcgcgggt ggcgaaagga tttatcaaac tcaggaatgt attcgctatt
    34741 attttttttc gtttccatgc ttttccagtt tcggataagg caaaaatcaa tctggtgata
    34801 gtgtagcggc gcaacttgcc ccgcaccaaa taaaaaagcc ggtactgact gcgtaccggc
    34861 tgcgaatgga tgttaattaa tcaaaccgta gctgcggcac aatctctttg gcctgtgcca
    34921 ggaattcgcg acgatcggag ccggtcagcc cttcggtacg cggcagtttt gccgtcagcg
    34981 ggtttacggc ctgctggttt atccatactt catagtgcag atgcggcccg gttgaacgtc
    35041 cggtattacc ggaaagcgcg atacggtcgc cacgtttcac cttctgtccc ggtttcacca
    35101 ggatcttgcg caagtgcata taacgcgtgg tgtagctgcg accatgacga atagccacat
    35161 aataacctgc tgcgccacta cgtttggcaa ccaccacttc accgtcaccc actgaaagca
    35221 ctggcgtacc ttgtggcatg gcaaaatcaa cacctctgtg tggcgcaacg cgaccggtca
    35281 ccggattagt acgacgcggg ttaaagttag atgagatacg gaactgtttc gccgtcggga
    35341 atcgcaagaa tcccttcgcc agaccagtac cgttacgatc atagaatttg ccatcttcag
    35401 cgcggattgc gtaataatct ttaccttctg aacgcaaacg tacgcccagc agctggcttt
    35461 gctcacgttt accatcaagc atttctcgtg acattaacac cgcaaattca tcgccttttt
    35521 tcagtttgcg gaaatccatt tgccactgca tggctttaat cactgcgctc acttcggcgc
    35581 tggttaaacc ggcgtttctg gcgctagcaa caaagcttcc cccgacggta cctttcagca
    35641 gattgttgac ccactctcct tgctgcattt cgctggtcat tttaaaaccg ttagcggcag
    35701 tacggtcata ggttcgggtt tcacgacgag acacttccca ggtgaggcgc tgcagttcgc
    35761 cgtccgcggt taatgtccag gagagttgtt gaccgatttt caggttacgc aattctttgt
    35821 cggcagcagc cagttgggtg atatcaccca tatcaatacc atactgattg agaatgctgc
    35881 ttagcgtatc gccagtggaa acaacatatt catgcacgcc cgcttcaccg gcgattttgt
    35941 catccagttc gtcctgggga atggcttcat cttcttgtgc agcttgatca atcggctcac
    36001 tggcttcagg taagagcgaa cgaatttcgt tctgttccag ctcaatggtt ttgacaattg
    36061 gcgtggcatc gcggtgataa acatagggcc gccagacagc gacggccaga gtaagaacgg
    36121 tgagcgaccc caacataaca cggtgtggtc gcggtaaatt attaaacgcc agggcgacag
    36181 agcgggctat ctgttgcacg taatcacttc ctcattaatc tcctttcagg cagctcgcat
    36241 actggttggc taattgattc aggaattctg aatagcttgt tttacccagt ttgatattcg
    36301 tccccagggg atccaacgtt cccatacgaa cggatgtccc tcgtgcgacg ctctcaacga
    36361 ccgctggcct gaactgtggc tcagcaaaaa cgcaggttgc tttttgctca accaactgtg
    36421 ttcttatttc atgtaaacgc tgcgcgccag gttgaatctc agggttaacg gtaaaatgac
    36481 caagcggtgt cagtccgaac tgtttttcga agtagccgta agcatcgtga aaaacgaaat
    36541 aacctttccc cttgagcggc gcgagctcgt taccaacctg cgtttcggtt gaggctaatt
    36601 gtgcctcaaa atccttcagg ttgtcgtcaa gtttggctcg actttgcggc ataagttcca
    36661 ctaattttcc atggattgca accgctgtag cccgcgctat ctctggggaa agccaaagat
    36721 gcatgttgaa atcgccgtga tggtgatctt cgtcactttt ttccgcgtga tcgtgatcat
    36781 catcatcgtc gtgaatactt ttcatcagca gcggtttcac atcttctagc tgcgcaatcg
    36841 ttacctgttt cgcttctggt aatttactta ccggtttttg catgaacgct tccatctccg
    36901 ggccaaccca aacgactaag tccgcgttct gtaagcgttt tacatccgat ggacgcagtg
    36961 aataatcatg ttctgaagcg ccgtcaggca gtaaaacctg cgtttctgtt accccatcag
    37021 caatggcaga agcgatgaac ccaacgggtt taagcgaagc gacaacggcg gcatctgcgg
    37081 cctgtgttgc accgccccag agagcggcgg ataatgctgc gaaaagaagc gtttttttat
    37141 gtaacataat gcgaccaatc atcgtaatga atatgagaac tgtgatatta taacatttca
    37201 taactactgc aagactaaaa ttaacatgac aagtctggtt tccctggaaa atgtctcggt
    37261 ttcttttggc caacgccgcg tcctctctga tgtgtcgctg gaacttaaac caggaaaaat
    37321 tttgacttta cttgggccaa acggcgcagg taagtcgaca ctggtacggg tagtgctcgg
    37381 gctggtaaca cccgatgaag gggttatcaa gcgcaacgga aaactgcgca tcggctatgt
    37441 accgcagaag ctgtatctcg acaccacgtt gccgctgacc gtaaaccgtt ttttacgctt
    37501 acgccctggc acacataaag aagatatttt gcctgcactg aaacgtgtcc aggccgggca
    37561 tctgattaac gcaccgatgc aaaagctctc gggtggcgaa acgcagcgtg tactgttagc
    37621 gcgagcattg ttaaatcgac cgcaattatt agtgctggat gaacccactc agggcgtgga
    37681 tgtgaatggt caggtggcgt tatatgacct tattgaccaa ctgcgtcgcg aactggattg
    37741 tggcgtttta atggtatctc acgatctgca tctggtaatg gcaaaaaccg atgaagtgct
    37801 ttgcctgaat caccacattt gttgttccgg cacaccggaa gttgtttccc tgcatccgga
    37861 gtttatttct atgtttggtc ctcgtggtgc tgaacaactg ggtatctatc gccatcatca
    37921 taatcatcgt cacgatttac agggacgaat tgttttgcgt cggggaaatg atcgctcatg
    37981 attgaattat tatttcccgg ttggttagcc gggatcatgc tcgcctgtgc cgcgggtccg
    38041 ctgggttcgt ttgtagtctg gcgtcgtatg tcttatttcg gtgatacgct ggctcatgcc
    38101 tcattacttg gcgtcgcgtt tggtttgttg ctggacgtga atccattcta tgcggtgatt
    38161 gcagttacgc tgctgctggc gggcggtctg gtatggctgg agaagcgtcc acagctggcg
    38221 atcgacacgt tattagggat tatggcgcac agtgccctgt cgctgggcct ggtggtcgtt
    38281 agtctgatgt ctaatattcg tgttgatttg atggcttacc tgttcggtga tttactggca
    38341 gtgacgccag aagatctcat ctctattgcg attggcgtgg tcatcgtggt ggctattttg
    38401 ttctggcaat ggcgcaattt gctgtcgatg acgattagcc cggatctggc gtttgttgat
    38461 ggtgtgaaat tacagcgcgt gaaattgttg ttgatgctgg tgacggcatt gacgattggt
    38521 gtagcgatga aatttgtcgg cgcgttgatt attacttcac tgctgattat tcctgctgct
    38581 actgcacgtc gctttgcccg cacgccggaa cagatggctg gtgtcgctgt tttggtgggg
    38641 atggtggcag tgactggcgg tttaaccttt tccgcatttt acgatacacc tgcaggcccg
    38701 tcggtggtgc tatgcgcggc actgttattt attctcagta tgatgaaaaa acaggccagc
    38761 taatctttcg ctgaacatat ttgtcggatg cggcgcgagc gccttatccg acctacggtt
    38821 cgctatctct ggtaggcctg ataagacgaa cagcgtcgca tcaggcacac tgccagtgtc
    38881 ggatgcggcg cgagcgacca atccgactta cggcatttct ggcggcgtta tgccaaagtg
    38941 attccacgcc cgcgtcgtcg ccatacgccc acgcggtgta cgctgcaaaa agccttgctg
    39001 aatcaaataa ggttccagta catcctcaat ggtttcacgt tcttcgccaa tggctgccgc
    39061 caggttatcc agacctaccg gcccaccaaa gaacttatcg attaccgcca gcaacaattt
    39121 gcggtccata taatcgaaac cttcagcatc gacattcaac atatccagcg cctgagcagc
    39181 gatatctgcc gagatggtgc catcgtgctt cacttcggcg aaatcacgca ctcgacgcag
    39241 cagacggttg gcaatacgtg gcgtaccgcg cgcacgacga gcaacttcca gcgcgccgtc
    39301 atcactcatc tcaagcccca taaagcgtgc gctgcgactg acgatatatt gcagatccgg
    39361 cacctgataa aactccagac gttgcacaat accaaaacga tcgcgcaacg gtgatgtcag
    39421 cgaacctgcg cgcgtggttg caccaatcag ggtaaacggc ggcaaatcaa ttttaatgga
    39481 gcgtgccgcc ggaccttcac caatcatgat atccagttgg taatcttcca ttgccggata
    39541 caacacctct tccaccactg gtgaaagacg gtggatctca tcaataaaca gtacatcgtg
    39601 tggttcaagg ttagtgagca ttgctgccag atcgcccgcc ttttccagca ccggaccaga
    39661 agtcgtgcgt aaattaacgc ccatttcatt ggcgacaata ttggcaagcg tagttttacc
    39721 caaccccgga ggaccaaaaa tcaatagatg atcgagggca tcgccgcgca gtttcgctgc
    39781 tttgatgaaa atctccatct gcgaacgaac ctgcggctga ccaacatact cttccagtaa
    39841 tttagggcga atggcgcgat ctgccacatc ttccggcaaa gtggtaccgg cagaaatcag
    39901 acggtctgct tcaatcatcc tttacctcat aacgcggcgc gtagggcttc gcgaattaat
    39961 gtttcactgc tggcgtcagg gcgagagatt ttgctcacca tgcggcttgc ttcttgtggt
    40021 ttatagccca gtgccaccag cgcagcaacc gcttcctgtt cagcatcgtc ggtcgccggg
    40081 ctggcaggag acgtgagtac caggtcggcg gctggcgtaa agagatcgcc atgcaaacct
    40141 ttaaatcggt ctttcatttc gacaatcaag cgttcggcgg tttttttgcc aatacccggc
    40201 agtttcacca gtgcccccac ttcttcacgc tcaacggcat taacgaactg ctgcgctgac
    40261 attccggaga ggatcgccag cgccaacttc gggccgacgc cgttggtttt gatcaactct
    40321 ttgaacaacg tgcgctcttg tttattgtta aaaccgtaca gcagttgcgc gtcttcacgc
    40381 accacaaagt gggtgaaaac gatcgcttcc tgacccgctt cagggagttc ataaaaacag
    40441 gtcatcggca tatgcacttc atagcctacg ccgcccactt caattaacac cagcgggggt
    40501 tgtttttcaa tgatgatgcc tctgagtctg cctatcacat gacgctcctg cgtaatgaat
    40561 caaagataat gctgtatgat aaaaaaatgc tggatagata tccagcgaag gatgaagaaa
    40621 acttgcgagg tgtctcgatg atctgaaaaa tggcgcagta taatttattc tacaggttat
    40681 attggaagca aatatttaaa tattacatat tcagcgaaga aatgtgtaat aaaaatacac
    40741 atcgcgaccc ctgaaaaaaa aataaatttt ttatgctatt acgtatattc atatctattt
    40801 caatggaatg acaacgtgaa tattaattat cctgctgaat atgaaattgg tgatatcgtc
    40861 tttacatgta taagtgctgc cttatttggt caaatatcag ctgcatcaaa ttgctggagt
    40921 aatcacgtcg ggatcattat cggtcataac ggtgaagact ttctggttgc agaaagccgt
    40981 gttcccctct caaccatcac tacgctatcc cgttttatta aacgctctgc taatcaacgc
    41041 tatgctataa agcgattaga cgccggacta acagaacaac aaaaacaacg aattgttgaa
    41101 caggttcctt cccggctacg caaactttac cacaccggtt ttaaatacga atcttcgcgc
    41161 cagttctgtt caaaatttgt ttttgatatt tataaagagg cgctatgtat tccggtgggt
    41221 gaaatagaga tgtttggaga attgttaaat agcaatccaa atgcaaaact cactttctgg
    41281 aaattctggt tcttaggttc tattccgtgg gagcgtaaaa ccgtcacgcc agccagtttg
    41341 tggcatcatc cgggtttggt gttgattcac gcggtgggag ttgaaacgcc tcagcctgaa
    41401 ctgaccgagg cggtataact taacgcagtc gccctctcgc caggttcagt cgcgattcgc
    41461 tcatttgcat cgcattctga ctaacgtggc agtgggtgat ggcaatcgcc agcgcatcgg
    41521 cggcatccgc ctgtggatta gcgggcagtt tcagcaaggt gcggaccata tgctgcacct
    41581 ggcttttttc ggcactacca atacctacca ctgtttgctt tacctgacgt gccgcatatt
    41641 caaataccgg caattcctga ttcaccgccg ccacaatcgc cacgccgcgc gcctgcccca
    41701 gttttagggc tgagtcagcg ttcttcgcca taaagacctg ttcaatggcg aaataatcag
    41761 gctggaattg ggtgatgatt tccgtcacgc ccgcatagat gagcttcaga cgagacggta
    41821 aatcatccac tttggtgcgt atgcatccgc tacccaggta ggacagttgc ctgcctacct
    41881 ggcggatgac gccatagccg gtcacgcgcg aacccgggtc aatgccgaga ataatagcca
    41941 tcacgcgtct ccgttttgct gtttagcagg cctcatcaga gagtcgctgc aacctcatca
    42001 gagatttcac cgttatggta aacttcctgc acgtcgtcgc aatcttccag catatcgatc
    42061 agacgcatca gtttcggtgc ggtttctgca tccatatcag ctttggtgga cgggatcatg
    42121 gaaacttccg cgctgtctgc tttcagacct gccgcttcca gagcgtcgcg tactttgccc
    42181 atttcttccc atgcagtgta gacatcaatc gcgccgtcat cataggtcac aacgtcttca
    42241 gcaccggctt ccagggctgc ttccatgatg gtgtcttcat cgcctttctc gaaggagatc
    42301 acgccttttt tgctgaacaa ataagctacg gaaccatcag taccgaggtt accgccacat
    42361 ttgctgaatg catgacgcac ttcagcaacg gtacggttgc ggttgtcaga cagacattca
    42421 atcatgattg ccgtgccgcc aggaccgtaa ccttcgtaga tgatggtttc catgtttgca
    42481 tcatcatcac cgcccacacc tcgtgcaatt gcgcggttca gagtgtcacg ggtcatgttg
    42541 ttagacagtg ctttatcaat tgctgcacgc aaacgcgggt tagcgtccgg atcaccaccg
    42601 cccagcttag ccgcggttac cagctcacga atgattttag tgaagatttt accgcgctta
    42661 gcatcctgcg cagctttacg atgtctggtg ttggcccatt tactatgacc tgccataaaa
    42721 atatctccag atagccctgc ctgttcaggc agcgttaatt acaaactgtt caatcgcctg
    42781 ccggttgctc caggacttag tgagcgccgc cgcagcagac gcatcaagcc acttgtaagc
    42841 cagatgttca gtgaaaacga tctggcgctc gtgcggaagc gcaagacaga accatgattc
    42901 cgtattacgc gtcacgcccg gcgcatagcg atgacgtaaa tgtgaaaaaa tttcaaactc
    42961 taccgtgcgc tgacagtcaa ttaaggtcag ttgttcagcg acaacatcaa tggtgacctc
    43021 ttcctttact tcgcgcatgg cagcttgcgg cgcggtttca ccctcttcca cgctgccggt
    43081 taccgactgc cagaaatcgg gatcgtcacg ccgctgcaac atcagcaccc gtttcgtatc
    43141 ttgtgcgtag atgaccacta agatcgaaac gggacgctta taagccatat cagttattct
    43201 cagccttctt cacaacctga atgctcagct cagccagtgc agtcaggtta gcaaagctcg
    43261 gtgcttcagt catcagacac gctgccgccg tggttttcgg gaaggcgata acgtcacgga
    43321 tattgtcggt gccggtcagc agcatcgtca gacggtcaag accgaatgcc agacctgcgt
    43381 gcggcggagt accgtatttc agggcgtcga gcaggaagcc gaatttctcg cgctgttcct
    43441 cttcgttgat acccagaata ccaaacaccg tctgctgcat atcaccatta tggatacgca
    43501 cagaaccacc gcccacttcg taaccattga tgaccatatc gtaagcgtta gccaccgcat
    43561 tttccggtgc agctttcagt tctgctgccg tcatatcttt cggtgaggtg aacggatggt
    43621 gcattgctgt caggccgcct tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca
    43681 gcggtgccca tttgctttcg tcggtcagac caaggtcttt acccactttc aggcgcagtg
    43741 cgcccatcgc gtcggcaaca attttcttgt tgtcggcacc gaagaaaatc atatcgccat
    43801 cttgcgcgcc agtacgctcc aggatggctt cgatgatttc tgcattaagg aacttcgcta
    43861 ccgggctatt gataccttcc agacctttcg cgcgttcgtt aactttgatg taagccagac
    43921 ctttcgcgcc gtagatttta acgaagttac cgtattcgtc gatctgctta cgggtcaacg
    43981 atgcgccgcc cggaacacgc agagcggcaa cacggccttt cggatcgttc gccggacctg
    44041 caaatactgc aaactcaaca gatttcagca gatcggcaac gtcggtcagt tccatcgggt
    44101 tacgcagatc cggtttatca gaaccataac ggcgttctgc ttctgcaaag gtcattaccg
    44161 ggaaatcgcc cagatccacg cccttcactt ccagccacag atgacgcacc agcgcttcca
    44221 tcacttcacg cacttgcggc gcggtcatga aagaagtttc cacatcgatc tgagtaaatt
    44281 caggctgacg gtcagcacgc aggtcttcgt cacggaagca tttaacgatc tgatagtagc
    44341 ggtcaaagcc ggacatcatc agtagctgtt tgaacaactg cggggattgc ggcagcgcgt
    44401 agaatttacc tttgtgcaca cgagaaggca ccaggtagtc acgcgcacct tccggcgtgg
    44461 ctttggtcag catcggagtt tcgatgtcga ggaagccgtg gtcatccata aaacggcgca
    44521 ccaggctggt gattttagcg cgggttttca ggcgctgagc catttccggg cgacgcaggt
    44581 cgaggtagcg gtatttcaga cgcgcttctt cggtgttgac gtggttagag tcaagcggca
    44641 gaacatctgc acgattgatg atagtcagcg aggacgccag tacttcgatt tcgccagtcg
    44701 ccatatcgcg gttaatattt ttttcgtcac gcgcacgtac ggtgcccgtg acctgaatgc
    44761 agaactcatt acgcagttca gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa
    44821 atacctgcac gataccttcg cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac
    44881 gacgacggtt gacccaacca cacagagtca cctgctgccc cacgtgggac aaacggagct
    44941 gtccacaata ttctgtacgc atgagatatc ccttaactta gctgccggcg gatgccccct
    45001 gctgcgcagg tgaccaagtc gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg
    45061 gctattatac tggaaattct gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt
    45121 taccacttta tatcgggcct gaaatcagac tctacgccag tttgctataa aggtgttgcc
    45181 cgaactcata aaaattaaca aaatttgtcg ttccgccatc ggctaatcgc attaaggtga
    45241 gaggcacgat tttgttttgt caggagtcat catgcctgaa cttaatgcta aaaccaccgc
    45301 gctggtggtg attgatttac aagaaggcat cttacctttt gccggaggtc cacatactgc
    45361 cgatgaggtg gttaatcgcg ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc
    45421 cgtgtttctg gtgcgcgttg gctggtctgc cgattacgcc gaagcattaa aacagccggt
    45481 tgatgccccc tcccccgcaa aagtgttgcc cgaaaactgg tggcaacatc ctgctgcatt
    45541 aggtgcaacc gacagcgata tcgaaatcat caaacgtcaa tggggtgcgt tttacggtac
    45601 ggatctggag ttgcaattac gccgccgggg tatcgataca atagtgttat gtgggatctc
    45661 gaccaatatc ggtgttgaat ccaccgcccg caatgcctgg gaactcggtt ttaatctggt
    45721 gattgccgaa gatgcctgta gcgccgctag cgccgagcag cacaataaca gcattaatca
    45781 tatctacccg cgcatcgccc gtgtgcgcag tgtggaagag atcctccacg cgttatgatt
    45841 tacatcggtc tgccgcaatg gtcgcatcct aaatgggtgc ggttggggat caccaacctt
    45901 gaagagtatg cccgccactt taactgcgtg gagggtaaca ccacgcttta tgccctgccg
    45961 aaacccgagg ttgtcctgcg ctggcgtgag cagaccacag atgacttccg cttctgtttt
    46021 aagtttccgg cgaccatttc gcatcaggca gcattacggc attgcgatga tttagtgact
    46081 gaatttttga cccgcatgtc accgttggct ccgcgcattg gacaatactg gctgcaactg
    46141 cctgccacat tcggcccacg ggagctgcct gcgctttggc attttctcga ttctcttccc
    46201 ggcgaattta attatggtgt ggaagtccgc catccacagt ttttcgccaa agaggaagag
    46261 gaacaaacgc ttaatcgcgg tttacatcag cgcggcgtta atcgggtgat tttagacagc
    46321 cgcccggttc atgcagcacg tccacacagt gaagctattc gcgacgctca acgaaaaaaa
    46381 cctaaagttc cggtacatgc tgtactgacg gcgaaaaatc cactgatccg ttttatcggt
    46441 agtgatgata tgacgcaaaa ccgggaatta tttcaggtct ggttacaaaa attagcacag
    46501 tggcatcaga ccactacgcc ttatcttttt ttacatacgc cagatattgc ccaggccccg
    46561 gaactggtac ataccctgtg ggaagactta cgtaaaacgc ttccagagat cggagcagtt
    46621 ccggctattc cacagcaatc ttctcttttc tgaatttgcc acctatcata gacaggtgcc
    46681 atcggccatt ttaaagggag tttgtatggt aagcgcgctg tatgccgttt taagtgcgtt
    46741 gttattaatg aagttctctt ttgatgtcgt tcgcctgcga atgcagtacc gcgttgccta
    46801 tggcgacggc ggttttagcg aactgcaaag cgctattcgc attcatggta acgcggtgga
    46861 atatattcct atcgcgattg tgttgatgct gtttatggaa atgaatggcg cagaaacctg
    46921 gatggtgcat atttgcggca tcgttttgct tgctggtcgt ctgatgcatt attacggttt
    46981 tcatcaccgt ctgttccgct ggcgacgttc tggcatgagc gccacctggt gtgcgctgtt
    47041 gctgatggtg ctggcgaatc tttggtatat gccctgggag ttggttttct ccctgcgtta
    47101 gcgcacaata cgccactttc tttttcccgg atttttacgt tatgtctcac cgcgacacgc
    47161 tattttctgc ccctatcgcc agactgggcg actggacctt tgatgaacgg gtagctgaag
    47221 tcttcccgga tatgatccag cgttccgttc ccggctattc caatattatt tccatgattg
    47281 gtatgttagc cgagcgcttc gttcaacctg gtacgcaggt ttacgatctg ggttgttctc
    47341 tgggcgcggc gacgctctcg gtgcgtcgca acattcatca tgataattgc aaaattattg
    47401 ccatcgataa ctccccggcg atgattgaac gctgccgtcg tcatattgac gcctataaag
    47461 ctcctacgcc agtagacgtt attgaaggtg atattcgcga tatcgccatt gaaaacgcat
    47521 cgatggtggt gctgaatttt accctgcaat tcctggaacc ttccgagcgc caggcgttac
    47581 tggataaaat ttatcaaggg ctgaacccgg gcggtgcgct ggtgctttcg gaaaaattca
    47641 gtttcgaaga tgccaaagtt ggtgaattgc tgttcaacat gcaccacgac tttaaacgtg
    47701 ccaacggtta cagcgaactg gagatcagcc agaaacgcag catgctggaa aacgtgatgc
    47761 tgaccgattc cgtggaaacc cataaagcac gcctgcataa agccggtttt gagcatagcg
    47821 agctgtggtt ccagtgcttt aactttggtt cactggtggc attaaaagca gaggacgctg
    47881 catgatcgac tttggtaact tttattctct gattgccaaa aatcatcttt cacactggct
    47941 cgaaacgctg cccgcgcaga ttgctaactg gcagcgcgag cagcagcacg ggctgtttaa
    48001 gcagtggtcc aacgcggtgg aatttctgcc tgaaattaaa ccgtatcgtc ttgatttact
    48061 gcatagcgtg acggcagaaa gcgaagaacc actgagcgcc gggcaaatta aacgcattga
    48121 aacgctgatg cgcaacctga tgccgtggcg caaagggccg ttctcactgt atggcgtcaa
    48181 catcgatacc gaatggcgtt ccgactggaa atgggatcgc gttctgcccc atctttctga
    48241 tttaaccggg cgcaccattc ttgatgttgg ctgcggcagc ggttatcaca tgtggcgcat
    48301 gattggcgca ggcgcgcacc tcgcggtggg gatcgaccct acgcaactgt ttctgtgcca
    48361 gtttgaagcg gtgcgtaaac tactgggcaa cgatcagcgc gcacatttgt taccgttagg
    48421 tattgaacaa cttccggcac tgaaagcctt tgataccgtc ttttcgatgg gcgtgcttta
    48481 tcatcgtcgt tcaccgctgg agcatctctg gcagttaaaa gaccaactgg tgaatgaagg
    48541 cgaactggtg ctggaaacgc tggttattga tggcgatgag aacacggtgc tggtgccggg
    48601 cgatcgttac gcacaaatgc gtaatgtcta tttcattcct tccgcgctgg cgctgaaaaa
    48661 ctggctgaag aagtgtggtt ttgttgatat tcgcattgca gatgtgagcg ttaccaccac
    48721 agaagagcag cgacgcaccg aatggatggt caccgagtct ctggccgatt ttctcgaccc
    48781 gcatgatccg ggtaagacgg tggaaggtta tcctgcgcct aaacgcgcgg tgctgattgc
    48841 gcgcaagccg taaaggtctg gtaatactgc cggatgcggc gtgaacgcct tatccggcct
    48901 acaaagtctt gctaattcaa tatattgcag gggctatgta ggcctgataa gcatagcgca
    48961 tcaggcagtt ttgcgtttgc ataacctcag cgcccgtttc cgggcgcttt tcacgtctta
    49021 tgcctgaaca gctggctgat caaacgccgt taacttcagc gcattgcccg tatatttttc
    49081 gatatacacc agcgcagagt taccggcaca ggcattcgcc agctgcgagc tggggatatc
    49141 cgccgttaac acgttcgcac taccgttttt acacaagcca ttttccagat ctggccatgc
    49201 accttcatgc acgcatacca cgcctttttt gatcccgtca gtcaccaccg cgcctgtcag
    49261 gatctgaccg agcttgttcc agacacgcac cagatcgcca ttcgcaatac caaagcgagc
    49321 agcatcttcg gtgtgaatag tgattggttc acgatcggca accgcatatt ttttacgcag
    49381 ttccgcatag ttaagctgac tgtgtaaacg gtgtgccgga tgtgcggtca gaagctgcaa
    49441 ctgcttctcg tcggcggtac ccttccactc atcaggcgca agccaggttg ggtgtgccgg
    49501 gcaatcctta tagccaaatt tttccagcgt tctggagtaa atctcaattt tgccgcttgg
    49561 cgtacccagc gcatttttca ccggatcggc gcggaaatca ccataacgaa tgtactgttc
    49621 gttcttctcg ctgcggcgca tttcgatcag tttattttgc tgccagaagg cattaaacat
    49681 cggcatagtc acgcgttgcg cacgggcacc tttctgagcg gcatcataga aaaatttcag
    49741 ccacgccatt tcatctttac cttcggtata gatctctttt ccgccaggtt tgagtaattc
    49801 agcaagatcg gcaaacacgt caaaatcgtt gcgcgcttca aattgcgcag cgacagcctg
    49861 cttcatcggc acaatatgct ggttgctgta atcaccggtc atcgtcaggt cattgcgctc
    49921 aaacgatgtg gtgatcggta atacgatatc tgcgtgtttg gctgctgcgg tccagtagca
    49981 ttcagaaacg acgatcatct ccggtttctg ccatgcttta atcagacgat tagtatcctg
    50041 gtgatgggta aagttaccgc cacccgccca ccagatcatt ttgatattcg gatacgtttg
    50101 ttctttaccg ttatgctgat atttgccgct aggattttcc agcgcatcga cgatacgggc
    50161 aacaggaatc gccgtcattc cgccatcatc agcggcctcg ctggcctgtc cggcaatcgc
    50221 cgcagacatt tctggtagca cgccgccaac acgcgtcggg ttgccaccgt tggagtaatg
    50281 ataagagaaa ccaaacgcgc cgcccggtgt accaatctgc cctaacatcg ccgccagcgt
    50341 caccagcatc cagtgtttct gttcaccata ttgctggcgc tgaattcccc agcctgccat
    50401 cagcattgtg cgatttgccg ccatcagttc agcgagtttg actatctgcg cttccggtac
    50461 gccagtaatt tccgccgccc aggccgcgct cttcggcgta ttatcgctct tacctgtcag
    50521 atactcttca aactgcggat acccggtagt gtatttttca aggaacactt tatcgtgttt
    50581 gccttgtgtc atcagggtat gcgcaatccc taacatcagt gccacatcgg tgcccatatt
    50641 cggtgcgatc caggtggcat tatcgccaaa gaattcgatg gtttcggagc agataggatc
    50701 aatggcaatc actggtttgc cagatttttt cagctgatgg aagtattcca gcccttgctc
    50761 atcggtactg ctccaggcaa ttttgagcgt gttcagcggg ttcatccccc acagcaccac
    50821 cacctggctg ttttccagaa taagcggcca ggaggtctgc tgttcataca cctctacaga
    50881 accgaccaca tgcggcatga tcacctgtgc cgctccggtt gaataatcgc cgctatgccc
    50941 ggaataaccg cccgccaggt tcatataacg ttgcagtaag gtttgcgctt tatgcaacac
    51001 gccagaagaa cgccagccgt aagatccggc aaaaatggcc gatggtccgt tagctttacg
    51061 aatacgatcg tgttgttcat gaatcagttt taatgcttgt tcccagctca cctgtacata
    51121 ggtatcttcg ccacgacctt tcgccggttg cagtggatta tcgagatagc tttttctcac
    51181 catcggatgc tgaatacgcg ccgtggtgtg tacctgatcc gccgccgtag actgtaagga
    51241 gttcggtatg gttttcgcca gcgcgcctgt cgaagaaaca atcttgccgt ccttcacttc
    51301 tacgttcatc gctccccaac ggcccgcagt gagaattttg ccgcccttct cttctgccca
    51361 tgcgggtagc ggttcagccg atgtcaccac cagcgtccct gccgcaatac cgctatgttt
    51421 aataaattca cgtcttgtta atgtcataac ttcctccctg atcaacgagg atcactgttt
    51481 ctcggtaata tctttggcgt tgtactggaa gtaccgcgtc aaaatgtcca gttcgttttc
    51541 gctcatgctg gttcgcgccc ccattccttt ggcaatggac ggccatgcat tgacggtgta
    51601 atggtcggcg gcaatagggg catggcagcc agcgcaatag gtatcgtcaa gtttttcagc
    51661 gtattgccat agcggtttac ggtccgctaa tgcgggatcg ataagcgccc cctgcaaaga
    51721 cgcctgacgc cattgattgc cgtattcgtc agcctgccat tccccgttta ccgttagcgc
    51781 cttgatacct tcttcactta atgtggcgag cgccagccgt tgacctgccg ccaggtagag
    51841 cgtgttttca ctgccctgca tttgataacc ctgcaaccga acaattggct gtttaccact
    51901 gacatcaacg acggtaagat cggtaccagg agtcacggta gccaggtcgc ctatgcgaga
    51961 agttttgaaa ggataaatat gtgcgccatt agtaactgaa gtagcggcct gactttccag
    52021 ctcatgcgcc gcgttgtcat ccatttttat ttctggcgga aaatgggcaa tgcctttatg
    52081 gcaatcgata caggtttcgc cgtccttttg cgctttatta tgcatttttt gcgcagattc
    52141 actttgcgag gcaatatcca tggcatcaaa agaatggcaa ctacggcagg ttgcagagtc
    52201 agttgctttt aattctttcc atactgtttc ggccatttcc tggcgatgag cttcgaactt
    52261 atcgtcactg tctattttgc cgctaacaaa ttcatgataa atatctttag atgcttttaa
    52321 tttagcaaat aaataatcca tccctgactt tggaatatgg caatcagcac attccgcacg
    52381 tattcctttc tggtttgaaa agtggacagt tccctgatat tcctcaaaag gtttactcat
    52441 cgagtggcag gaaaggcaaa atgcggtatc cgacgtttta tgtaaggctt tttgcgccag
    52501 caataacccg ccgccaccaa ccacaaccgc tatcagcaaa aacaataacc caatgcgttt
    52561 tttccctcgc atagattcct ctaattaaat tccaggacac cgataccatt gtataaataa
    52621 atacacaacg aattgagtac ttgcttaatt cggcacaaca ctgcct
//
LOCUS       DAZGJT010000023        51710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZGJT010000023 DAZGJT010000000
VERSION     DAZGJT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.023"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..1658
                     /gene="yegH"
                     /locus_tag="ACHKFK_001485"
     CDS             75..1658
                     /gene="yegH"
                     /locus_tag="ACHKFK_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9051855.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQLLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(1726..3579)
                     /gene="asmA"
                     /locus_tag="ACHKFK_001486"
     CDS             complement(1726..3579)
                     /gene="asmA"
                     /locus_tag="ACHKFK_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO9051856.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLMA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPMLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3601..4182)
                     /gene="dcd"
                     /locus_tag="ACHKFK_001487"
     CDS             complement(3601..4182)
                     /gene="dcd"
                     /locus_tag="ACHKFK_001487"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO9051857.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4274..4915)
                     /gene="udk"
                     /locus_tag="ACHKFK_001488"
     CDS             complement(4274..4915)
                     /gene="udk"
                     /locus_tag="ACHKFK_001488"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO9051858.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5233..8550
                     /gene="dgcE"
                     /locus_tag="ACHKFK_001489"
     CDS             5233..8550
                     /gene="dgcE"
                     /locus_tag="ACHKFK_001489"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9051859.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIIPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQSNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIA
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDGNNHQAAEVMSQADIACYASKNGGRGRVTIYEPQQAAAHNERAA
                     ISLDEQWRMIKENQLMMIAHGIASPRLPEASNLWLISLKLWSCESEIIDEQTFRRSFS
                     DPALSHALDQRVCHDFFRQAAKAVASKGLSIALPLSVAGLSSATLVNELLEQLENSPL
                     PARLLHLIIPAEAIFDHAESVQKLRLAGCRIVFSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     NAQPLDLLVNRSYFAIN"
     gene            complement(8660..9508)
                     /gene="alkA"
                     /locus_tag="ACHKFK_001490"
     CDS             complement(8660..9508)
                     /gene="alkA"
                     /locus_tag="ACHKFK_001490"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO9051860.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTSRVAQLYGERLDDFPDYVCFPTPQR
                     LAVADLQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            9642..10994
                     /gene="yegD"
                     /locus_tag="ACHKFK_001491"
     CDS             9642..10994
                     /gene="yegD"
                     /locus_tag="ACHKFK_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9051861.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANTQAQGILERAAKRAGFKDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANCRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11007..12947)
                     /gene="yegI"
                     /locus_tag="ACHKFK_001492"
     CDS             complement(11007..12947)
                     /gene="yegI"
                     /locus_tag="ACHKFK_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO9051862.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTMPSFVGFERTENHDNFGLALLIFHILFGGR
                     HPYSGVPLISDAGNALEMDIAHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDLLRQQLKKCTVSAMHVYPAHLADCPWCALDNQGVIY
                     FIDLSEEVITTGGDFVLARVWAMVMASVAPPALQLPLPDHFQPTGRLLPSGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILIPVLAAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYDHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRSVKQVRGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAVTPADRQAVMAKMAAKRHRLESALTVGATELQRFRLQAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(12944..13705)
                     /gene="pphC"
                     /locus_tag="ACHKFK_001493"
     CDS             complement(12944..13705)
                     /gene="pphC"
                     /locus_tag="ACHKFK_001493"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HFO9051863.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLSVFV
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDVLATDIVLTIRQRLFAEAEA
                     KELAIRDFACTFLGLISSANGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKLFLSSPAVNERTDDDKTLALAMWLP"
     gene            complement(13702..14361)
                     /gene="yegL"
                     /locus_tag="ACHKFK_001494"
     CDS             complement(13702..14361)
                     /gene="yegL"
                     /locus_tag="ACHKFK_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9051864.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFTIG
                     VQGADMKTLAQISVRQPLSLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14579..14635)
                     /locus_tag="ACHKFK_001495"
     CDS             complement(14579..14635)
                     /locus_tag="ACHKFK_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9051865.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            complement(14907..14963)
                     /locus_tag="ACHKFK_001496"
     CDS             complement(14907..14963)
                     /locus_tag="ACHKFK_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO9051866.1"
                     /translation="MMKWIIIVVLLVISFPAY"
     gene            15242..16489
                     /gene="mdtA"
                     /locus_tag="ACHKFK_001497"
     CDS             15242..16489
                     /gene="mdtA"
                     /locus_tag="ACHKFK_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO9051867.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSATPGATKQAQ
                     QSPAGGRRGMRAGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            16489..19611
                     /gene="mdtB"
                     /locus_tag="ACHKFK_001498"
     CDS             16489..19611
                     /gene="mdtB"
                     /locus_tag="ACHKFK_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO9051868.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSESVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGCGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            19612..22689
                     /gene="mdtC"
                     /locus_tag="ACHKFK_001499"
     CDS             19612..22689
                     /gene="mdtC"
                     /locus_tag="ACHKFK_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO9051869.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDDTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRARLPELQSTIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGAVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRGERSETAQ
                     QIIDRLRKKLAKEPGANLFLMAVQDIRVGGRQANASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQEDNGAEMNLIYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQAVTE"
     gene            22690..24105
                     /gene="mdtD"
                     /locus_tag="ACHKFK_001500"
     CDS             22690..24105
                     /gene="mdtD"
                     /locus_tag="ACHKFK_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051870.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQIGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGFSPLAIAGLVAVGVVALVLYLLHAQNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRWVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFVF
                     ARVPSDTHQNVAISRRKRSAQ"
     gene            24102..25505
                     /gene="baeS"
                     /locus_tag="ACHKFK_001501"
     CDS             24102..25505
                     /gene="baeS"
                     /locus_tag="ACHKFK_001501"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO9051871.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLITMHWAVRISFERGFIDYIK
                     HGNEQRLQMLGDALGEQYAQHGNWRFLRNNDRFVFQILRSLEHDNNEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPVPPDGTRRPIMVNGAEVGAVIASPVERLTRNTDINFD
                     RQQRQTSWLIVALSTLLAALATFPLARGLLAPVKRLVDGTHKLAAGDFTTRVAPTSED
                     ELGRLAEDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TLASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     LSITVELPLERDLQREV"
     gene            25502..26224
                     /gene="baeR"
                     /locus_tag="ACHKFK_001502"
     CDS             25502..26224
                     /gene="baeR"
                     /locus_tag="ACHKFK_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO9051872.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26404..26736
                     /gene="yegP"
                     /locus_tag="ACHKFK_001503"
     CDS             26404..26736
                     /gene="yegP"
                     /locus_tag="ACHKFK_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO9051873.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            26883..28244
                     /gene="yegQ"
                     /locus_tag="ACHKFK_001504"
     CDS             26883..28244
                     /gene="yegQ"
                     /locus_tag="ACHKFK_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO9051874.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKN
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGELAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(28744..29061)
                     /gene="yegR"
                     /locus_tag="ACHKFK_001505"
     CDS             complement(28744..29061)
                     /gene="yegR"
                     /locus_tag="ACHKFK_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO9051875.1"
                     /translation="MKKIAAISLISVFLMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDATHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            29476..30375
                     /gene="yegS"
                     /locus_tag="ACHKFK_001506"
     CDS             29476..30375
                     /gene="yegS"
                     /locus_tag="ACHKFK_001506"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO9051876.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAVRFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDEIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQINKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIITGDEIIPTLVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(30457..31230)
                     /locus_tag="ACHKFK_001507"
     CDS             complement(30457..31230)
                     /locus_tag="ACHKFK_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9051877.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSGVDKLALQKTRVKLITV"
     gene            complement(31336..32376)
                     /gene="gatD"
                     /locus_tag="ACHKFK_001508"
     CDS             complement(31336..32376)
                     /gene="gatD"
                     /locus_tag="ACHKFK_001508"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9051878.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLFPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRNNVFALPADMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSREMSAP
                     QIQGVLRDLRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(32424..33779)
                     /locus_tag="ACHKFK_001509"
     CDS             complement(32424..33779)
                     /locus_tag="ACHKFK_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO9051879.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIDLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYLGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIVIGAIYLTGIFMTWRRARGFIKQEKAVLA
                     E"
     gene            complement(33783..34067)
                     /gene="gatB"
                     /locus_tag="ACHKFK_001510"
     CDS             complement(33783..34067)
                     /gene="gatB"
                     /locus_tag="ACHKFK_001510"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO9051880.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(34098..34550)
                     /gene="gatA"
                     /locus_tag="ACHKFK_001511"
     CDS             complement(34098..34550)
                     /gene="gatA"
                     /locus_tag="ACHKFK_001511"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO9051881.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPEIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(34560..35822)
                     /gene="gatZ"
                     /locus_tag="ACHKFK_001512"
     CDS             complement(34560..35822)
                     /gene="gatZ"
                     /locus_tag="ACHKFK_001512"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO9051882.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFAIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAGRAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITQVEDAANTLRTHQKAFIARGLAEALTRVIA
                     IVVQPGVEFDHSNIIHYQAQEAQALAQWIEKTKMVYEAHSTDYQTQTAYRELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMFDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGMEIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGSAE"
     gene            complement(35851..36705)
                     /gene="gatY"
                     /locus_tag="ACHKFK_001513"
     CDS             complement(35851..36705)
                     /gene="gatY"
                     /locus_tag="ACHKFK_001513"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO9051883.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLTIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPVQA
                     REFAEATGIDSLAVAIGTAHGMYARAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(36932..37984)
                     /gene="fbaB"
                     /locus_tag="ACHKFK_001514"
     CDS             complement(36932..37984)
                     /gene="fbaB"
                     /locus_tag="ACHKFK_001514"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO9051884.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            38241..39518
                     /gene="yegT"
                     /locus_tag="ACHKFK_001515"
     CDS             38241..39518
                     /gene="yegT"
                     /locus_tag="ACHKFK_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO9051885.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGAVLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWAGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            39515..40519
                     /gene="yegU"
                     /locus_tag="ACHKFK_001516"
     CDS             39515..40519
                     /gene="yegU"
                     /locus_tag="ACHKFK_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO9051886.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILGWALRFDAFNKN
                     VLGPTSKIALNDIRDGKPVAELENNGVTNGAAMRISPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            40516..41481
                     /gene="yegV"
                     /locus_tag="ACHKFK_001517"
     CDS             40516..41481
                     /gene="yegV"
                     /locus_tag="ACHKFK_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO9051887.1"
                     /translation="MSSARLHTLLPELTPRQPVMVIGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEDLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(41455..42201)
                     /gene="yegW"
                     /locus_tag="ACHKFK_001518"
     CDS             complement(41455..42201)
                     /gene="yegW"
                     /locus_tag="ACHKFK_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9051888.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQSLEEEGVVTRSRGYGTQINNIFEYSLKEARGVS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(42253..43071)
                     /gene="yegX"
                     /locus_tag="ACHKFK_001519"
     CDS             complement(42253..43071)
                     /gene="yegX"
                     /locus_tag="ACHKFK_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO9051889.1"
                     /translation="MQLRITSRKKFTVLLCALGLISIVAIYPRQTVNFFYSTAIQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(43136..43936)
                     /gene="thiD"
                     /locus_tag="ACHKFK_001520"
     CDS             complement(43136..43936)
                     /gene="thiD"
                     /locus_tag="ACHKFK_001520"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO9051890.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAQRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(43933..44721)
                     /gene="thiM"
                     /locus_tag="ACHKFK_001521"
     CDS             complement(43933..44721)
                     /gene="thiM"
                     /locus_tag="ACHKFK_001521"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO9051891.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMSAAVEQATRSQTPWTLDPVA
                     VGALDYRRRFCVELLSHKPTAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVMVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENIASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(44756..44852)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(44944..45216)
                     /gene="rcnR"
                     /locus_tag="ACHKFK_001522"
     CDS             complement(44944..45216)
                     /gene="rcnR"
                     /locus_tag="ACHKFK_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO9051892.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            45337..46161
                     /gene="rcnA"
                     /locus_tag="ACHKFK_001523"
     CDS             45337..46161
                     /gene="rcnA"
                     /locus_tag="ACHKFK_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO9051893.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIISTA
                     FWMFWRTWRGERNWLENMHEHDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSLGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            46380..46718
                     /gene="rcnB"
                     /locus_tag="ACHKFK_001524"
     CDS             46380..46718
                     /gene="rcnB"
                     /locus_tag="ACHKFK_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO9051894.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(46800..47834)
                     /gene="yehA"
                     /locus_tag="ACHKFK_001525"
     CDS             complement(46800..47834)
                     /gene="yehA"
                     /locus_tag="ACHKFK_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO9051895.1"
                     /translation="MEIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLESIPVSQLPATGN
                     KTTINSKKGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGTPVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(47850..50319)
                     /gene="yehB"
                     /locus_tag="ACHKFK_001526"
                     /pseudo
     CDS             complement(47850..50319)
                     /gene="yehB"
                     /locus_tag="ACHKFK_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            complement(50335..51009)
                     /gene="yehC"
                     /locus_tag="ACHKFK_001527"
                     /pseudo
     CDS             complement(50335..51009)
                     /gene="yehC"
                     /locus_tag="ACHKFK_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(51090..51632)
                     /gene="yehD"
                     /locus_tag="ACHKFK_001528"
     CDS             complement(51090..51632)
                     /gene="yehD"
                     /locus_tag="ACHKFK_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9051896.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDGAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTADGQYAIPFKAKYLKLT
                     DNSVNSGDVLSSLIMRVAQD"
BASE COUNT        12464 a        13513 c        12846 g        12887 t
ORIGIN      
        1 ttgcattttt gccccgattt atccatgatc gaattgtgac atttgtcata caacgaatag
       61 gttttgtact tactatggaa tggattgccg atccgtctat ctgggctggg ttaatcacgc
      121 tgattgtgat cgaactggtc ctcggcattg ataacctggt ctttattgcc atcctcgccg
      181 aaaaactacc gcctaagcag cgcgaccgcg cacgggttac cgggctcctg ctggcgatgt
      241 taatgcgcct gttactgctg gcgtcaatct cctggctggt caccctgact caaccgctgt
      301 tcagcttccg ctcgtttacc tttagcgccc gcgacttaat catgctgttt ggtggtttct
      361 tcctgttgtt caaagccacg atggagctga acgaacggct ggaagggaaa gacagcaata
      421 accccaccca acgcaaaggt gcgaagttct ggggggtggt gacacaaatt gtggtactgg
      481 acgccatctt ctcactcgac tcggtgatta ccgccgtcgg gatggtcgac catttactgg
      541 tcatgatggc cgccgtggtt atcgctatca gcctgatgtt gatggccagc aagccgttaa
      601 cccagttcgt taacagtcac ccgaccatcg ttattctctg cttaagcttc ctgttgatga
      661 ttggctttag cctggtggcg gaaggtttcg gcttcgtcat tccgaaaggc tacctgtacg
      721 ctgccattgg tttctcggtg atgatcgagg cgctcaatca gttggctatc tttaaccggc
      781 gacgttttct ttccgctaat cagacgctgc gccagcggac taccgaagcg gtaatgcgcc
      841 tgcttagcgg gcaaaaagaa gatgcggaac tggacgccga aaccgcgtcc atgctggtgg
      901 atcatggcaa ccagcagatc tttaatccgc aggaacggcg gatgattgaa cgggtactta
      961 atcttaacca gcgtaccgtc agcagcatta tgacgtcgcg ccacgatatt gagcatatcg
     1021 atctcaacgc gccggaggac gagatccgcc agttactgga gcgaaatcag catacgcggc
     1081 ttgtcgttac tgatggcgat gacgcagaag atttgctcgg tgttgttcac gttatcgacc
     1141 tgctacaaca gttactgcgc ggcgaaccgc tcaacctgcg ggtgttgatt cgtcagccgc
     1201 tggtgttccc ggaaaccttg ccgttgttac ctgccctgga gcagttccgt aatgcccgca
     1261 cacactttgc ttttgtggtg gatgagtttg gctcggtgga agggattgtg acattaagtg
     1321 acgtcactga aaccattgcc ggtaacttac cgaacgaagt ggaagagatc gacgcccgtc
     1381 atgatattca gaagaatgcg gacggttcct ggacggcgaa tggtcatatg ccgctggaag
     1441 atctggtgca atatgtgccg ttgccgctgg atgaaaaacg tgaatatcac accattgccg
     1501 ggctattgat ggaatatttg cagcgtattc caaaacctgg cgaagaagtt caggtggggg
     1561 attatttgct taaaacgttg caggtggaaa gccatcgcgt gcagaaggtg cagattattc
     1621 cgctgcgtaa ggatggcgag atggagtacg aggtgtgatg gcatgtaggc ctgatgcgac
     1681 gctttacgcg tcttatcagg cctacaggac agctcacctg gtaacctaca tcttctccag
     1741 caacttcttc acatctttgc cattgcggga atctttattc cgctcggccc agtcgttaag
     1801 acggcgtttt gcttcatcct gtagatgttt acgcagtaac tgatccactt gcaggctgta
     1861 attaagttgc tgccagttgc cataaacccg cagcggtact ggcgtttctt tgaggaaatc
     1921 aatcagtttg ctttctccgt tccagccacc cacgacccga atatcaaact gggtgtcgca
     1981 ggtttgctcc gccagattca acgtaccagc cccggaaagc gccagcatgg gcgattgacc
     2041 ttgcatgtcg tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg taaagcggtc
     2101 aagacgcgtc acgttatcga agttttcagc ggccttcaca tcaccaccat tacgttctac
     2161 cgcttgctga atcatctgct ggaagttcat cccttccatg cgtgtgtcgg tcatttcgac
     2221 atgtgcctgt ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag caccggagaa
     2281 atcaccagcc agtgacatct ttccggtcaa cgaaatcgga tagttaaacg ccttcagaat
     2341 ggtgccaatc tcaacgtttt ccagccgtgg ctggaagttt atccgcggat ttattgatgt
     2401 cgcgtccagc gtgcccggca gtgaaacctg tccaccgtta agtttgccct gcagttgagt
     2461 aatttccagc aaacccgact tgttggtcat ttgcgtggca acatctgtaa aattcattcc
     2521 gcgccagcgc acgttactgg cctgcaacaa aatatcagcc gtaaagcctt gcagtccctg
     2581 ataggccggt tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg attggctctg
     2641 cccttgctgc gcggcaccgt tctcaccgtt cgctgtttca ttaaggggga tgaggttgtc
     2701 aagattcagt tgcgggaatt gcagtctcag ctgccattcc ggtttctctg tcaacgtgac
     2761 ctgtgcttgc ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt taaatgaaag
     2821 gcgtttatgt gactcctgcc actggacctg gaaactcccc tgcccctgaa taccttgttt
     2881 tggtaaatcg gcaccctgca actgccagtt aatttgttca atagccgcca ttaaatcatg
     2941 cggataatca gaagcatcca ccgtaccgtt aagggatata gtgagatcgc gctgatcgcg
     3001 attaacccgc ccggagaact caaatgagcc acgatgttgg ggatcttgtt ccatttgcag
     3061 gcggatattg cggattgtca cctgctcgtc atcttcatgc tggaacacca gaacgctgtc
     3121 cgccacctta agactggata tatcaaacga ccatccgcga tcgtctgaca gatccggcaa
     3181 ggtattgtcg cgcggtgcaa ccggagcgtc ttcactgcgc acagcttccg tctgcggcgt
     3241 cagttggatc actgccccct ttagcatcac ctgcttaacg ctcagttgat gactcagtag
     3301 tggtaaaagc gccacgtcca gacgcatgtt gtcggcgcga accagtggct ggcttgcgcc
     3361 ctgggcggtg agagacattc gcccggagag gatactaagc tgcggccaga cgtgccaacg
     3421 cagtggcccg tcgagctgca attgataacc gctacgtgca gcaacttgct tgaccatata
     3481 gtcgcggaaa tcattcggat tcaccagcaa cactaacgca gataacccgg ccaccagcac
     3541 gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa tgggcctgaa
     3601 ttagtcttta tcgattcggc tggctaccgc gccctgctgg ttgcgatatt tcgcatcttc
     3661 acggcggttg taaggtcgcg ccgccgggcc ggagagcggc tcaaagctca gcgcaccaat
     3721 taacatgccc ggacgcagcg ccagcggcag cttaccggag ttgtaaaact ccagcacaat
     3781 gcagccagac cagcccggat cgatacggtg cgcggtgacg tgcaccatca gccccagacg
     3841 cgccagtgaa gaacgcccgt ccagccagcc caccagatcg gctggcagcg tcaccgactc
     3901 cagcgtcacc gccagcgcca gctctcctgg gtgaagataa aacgcctcgc cctcgtcgag
     3961 cacaatttca tcgctcatca cgcggtcaag cgcggcgctc acttcatctt tgggaccgct
     4021 cagatcgata aacgctgccg tgtgaccacg gaaggtacga aatttattgc ccaggcgcac
     4081 atccaccgtc gcgccgttaa tacgctccac tggcggacgt gggttgatcg acaaacggcc
     4141 ttcatcaagc caggcttcaa tatctcggtc acacagacgc atggcatttt ctcctttcga
     4201 gcatcactcc cttaacgcca attacgtcaa gggcatacca ggttatcact gaacggtaca
     4261 caatttatca agcttattca aagaactgac ttattttcgc tttcaatata tcgatcgcga
     4321 tgcggttttt cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa ggctcaatga
     4381 attgcagaaa catcgggcgc acggtttttt gatattgcgc catcactgaa tccattgaac
     4441 gcccacgctc gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc agcggggtat
     4501 caacgaaaat ggagaagtta agttcgtcac gtaaacgcgc atccgtcagc aacaaaatgc
     4561 cttcgagaat gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata cgcgtatgtt
     4621 caacatagct gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct tgtaaatgct
     4681 caagcagcag gctgtgatcc atcgcgctgg gatggtcgta gttggtctta acgcgttctt
     4741 ccatcgacag atggctttga tctttgtaat agcagtcttc gggaattacg ccgatgtgtt
     4801 catcaccgac ttgctcacgt aattcacgat aaagggtact ggcaataaga ctcttgccgg
     4861 aagccgatgc gccagcgata ccgataatga cgcactgatg agactgatca gtcatatatt
     4921 tagcgacctg attaacctgg atgttaggaa gggggcgacg aagcgccaaa cgcggcaatt
     4981 atagggattt catccgcctg ataccagtcg aatagcgttg ccgcgcgctc agagttaatt
     5041 gttgacgaag aattcccggt ggcaaattac gttgatcagt tttatacaag gtaaaaaatg
     5101 ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat tcgcatataa
     5161 aatgtaaaac ttttgtacta gcataaacac agaaacgaat actggcgacc tggccttgcg
     5221 gataaagcgg taatgagcaa acaatcacag catgtattaa ttgccctgcc ccacccgctg
     5281 cttcacctgg tcagtttagg tttagtctcg tttatcttca cccttttctc acttgagctt
     5341 tcgcagtttg gcacccaact cgccccactg tggttcccga cctccatcat gatggtggcg
     5401 ttttatcgcc atgccgggcg catgtggccg ggaattgcgc tgagctgctc gctgggaaat
     5461 atcgccgcat ccatcctgct tttttccacc agctcgctga acatgacctg gacgaccatc
     5521 aatattgttg aagccgtggt cggggcagtg ctgctacgta aattgctgcc gtggtataac
     5581 cccttgcaaa atctggctga ctggctgcgt ctggcactcg gcagcgccat tattccgcct
     5641 ctgttggggg gtgttctggt tgtcctgctg acgcccggag acgatcctct cagggcattt
     5701 ttgatatggg tactgtcaga atccatcggc gctctggcac tggtgccgct gggattgtta
     5761 tttaaaccac actatctact gcgccatcgc aacccaaggt tgctttttga gtcgctgctc
     5821 acgttagcca tcacgctgac gttaagctgg ctttcgatgc tgtatctgcc gtggcctttt
     5881 actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat ggaagccttt
     5941 ttgatcttcc ttaccacagt gatgatggtg tcgctgatga tggccgcgga tccctccctg
     6001 cttgctacgc cgcgtacgta cctgatgagc catatgccgt ggctaccgtt tttgctgatc
     6061 ctgctgcccg ccaacatcat gacgatggtg atgtatgcct ttcgtgcgga acgcaaacac
     6121 atttccgaaa gcgaaacccg ctttcggaac gcgatggaat attccgctat cggcatggcg
     6181 ttagtgggca ccgagggaca atggctgcaa tccaacaaag cgctctgcca gtttctcggt
     6241 tacagtcagg aagagctgcg cggactcacc tttcagcaac tgacctggcc ggaggatctc
     6301 aataaagatc tccaacaggt tgaaaagctg ataagcggtg aaataaacac ctattcaatg
     6361 gaaaaacgtt actacaaccg caatggcgat gttgtctggg cgttgcttgc cgtctcactg
     6421 gtgcgccaca cggatggcac gccgctctat tttatcgctc agattgaaga cattaacgag
     6481 ctaaaacgca ccgaacaggt caatcagcaa ctgatggagc gcatcacgct ggccaacgaa
     6541 gcgggcggga ttggcatctg ggagtgggag ctgaagccga atatttttag ctgggataag
     6601 cggatgttcg agctgtatga aattcctccg catatcaaac cgaactggca ggtgtggtac
     6661 gagtgcgtgc tgccggaaga tcgccagcac gccgaaaaag tgattcgtga ttcgttgcaa
     6721 tcacgctcgc cctttaaact ggaatttcgc attgccgtga aagatggcat ccgccatatc
     6781 cgcgccctcg ccaaccgggt actgaataaa gaaggcgaag ttgaacgcct gctcggcatt
     6841 aatatggata tgacggaggt taaacagctt aacgaggcat tgtttcagga aaaagagcgc
     6901 ctgcacatta cgctggactc catcggcgaa gccgtggtct gtattgatat ggcaatgaaa
     6961 attaccttta tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcgtta
     7021 ggtgttccgc tcctgacggt gttgcatatt acttttggcg acaacggacc attaatggag
     7081 aacatttaca gtgctgacac ctcacgttcc gcgattgaac aagatgtggt attgcactgc
     7141 cggagcggcg gcagttacga cgtgcattac agtattacgc cgttaagtac tctggacggc
     7201 agcaatatag gctcggtact ggtgattcag gacgttaccg aatcacgcaa aatgctgcgc
     7261 cagctgagct acagcgcctc ccatgatgca ctgacgcatc tcgccaatcg cgccagtttt
     7321 gagaagcaac tgcgtatcct gctgcaaacg gtaaacagta cgcatcagcg acatgccctg
     7381 gtgtttatcg atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggc
     7441 gacgctttac tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc cagcgacgtg
     7501 ctggcgcggc tcggtggtga tgaatttggt ctgctactgc cagactgcaa tgttgaaagc
     7561 gctcgtttta tcgctacacg cattatcagt gccgtgaatg actatcactt tatatgggaa
     7621 ggacgtgtgc atcgggtagg tgccagtgcc ggaattacct tgattgatgg caacaatcat
     7681 caggcagctg aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtggc
     7741 cggggccggg tgacgattta cgaaccgcag caagctgccg cacataacga acgggcagcg
     7801 atatcgcttg atgaacagtg gcggatgatt aaagagaatc agttgatgat gatcgcccac
     7861 ggcatcgctt cgccacggct cccggaagca agtaatttgt ggctgatttc acttaagctc
     7921 tggagttgcg aaagcgagat tattgatgaa caaacatttc gtcgtagctt cagcgatccg
     7981 gcacttagcc atgctcttga ccaacgggta tgccacgatt ttttccggca ggccgcaaaa
     8041 gcggttgcca gtaaaggctt aagcatcgcc ctcccccttt ccgttgctgg tttgagtagc
     8101 gccacgctgg tgaatgaact gcttgagcag ctggaaaata gccctctacc agcacggtta
     8161 ttacatctga ttattccggc agaagcgatt ttcgatcacg cagaaagcgt gcaaaaactg
     8221 cggctggcgg gatgtcggat cgtattcagt caggtgggcc gagatctgca aatcttcaac
     8281 tcgctgaaag caaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg
     8341 cagggtaatt tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc
     8401 gggatgaaaa ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttcgggc
     8461 atcggcgtcg atctgattta tggtgatgtg attgccaatg cccaaccgct ggatttgctg
     8521 gtgaatagaa gttatttcgc gattaactaa gggagcgttt gcccattgcc tgatgcgacg
     8581 ctaacgcgtc ttatcaggcc tacaaatcgc tcattcccca ggccggataa ggcgctcgca
     8641 ccgcatccgg cgaccaacgt catgcttcgt ctggttgcca gccttccgta taccagatat
     8701 gcaacagcgc ataagaacgc cagggcttcc agcgttcggc ataacggcgg atttgtgccg
     8761 gcgtcattcc cggaaatcgc tgtttaatca gataatcatc cggcagaaaa acatctttcg
     8821 cctgccagcc acgcaaagca aaataatttg cagtccagcg cccgataccc ggaaaggttt
     8881 gcagcgtttt catcgcttgt tctacgtcgc ccggtattgt cattggtaag gtgccctcca
     8941 gcgccgcatt tgccagatga atcagcgcct ctgcccgttt caacggcata cctaacgctt
     9001 ttaatgcctg caggtcggct actgccagcc gctgaggcgt ggggaagcag acataatccg
     9061 gaaaatcatc cagccgttcg ccataaagct gtgccactct ggaggtcaat tttgccgcca
     9121 tcgccacgct caccagttgg cctaaaatcg cccgcacgcc ctgctcaaaa gcgtcaacac
     9181 aaccaggtaa acgcaatcct ggccgcgccg cgcctaactt gcccaacgca ccgttaacaa
     9241 tctgcgggtt acattgcaga tcaaacaggc ggctcatttt cgccagacac tcagcggcaa
     9301 caggttctaa acctgcactt aaatttatgt gcagagtatg gcgggctata tccggaatag
     9361 cagtcaccac gccgcgatat tcgcctaccg ccagactacg ggcataataa ctgtccgcga
     9421 ccgtttccac accgctcacc gcacgggcgg cgagaaatcc caacatccac gaccagtcat
     9481 acggcggctg ccagttcagg gtatacatcg catctccttg ttaatccgct ttcagcataa
     9541 acgttattca gacgttacgc tttgctttca tattccggtt gtcgcgacgg caacatttcg
     9601 ctaaagtcac gccccttctt caccggcatg gggattattt cgtgtttatt ggttttgatt
     9661 acggtacagc aaactgttca gtggcggtca tgcgcgacgg taaaccgcat ttgctaaaaa
     9721 tggaaaacga cagcacgctg ctgccttcaa tgctttgcgc gccaacgcgt gaagctgtaa
     9781 gcgaatggct gtaccgccat catgatgttc cggcagacga cgatgaaacg caggcgctgt
     9841 tgcgtcgggc gattcgttat aaccgcgaag aagatatcga tgttacggcg aaaagcgtgc
     9901 agttcggtct ttcctcactg gcacagtaca ttgatgatcc agaagaagtg tggtttgtga
     9961 aatcaccaaa atcgttcctc ggtgccagcg gcttaaaacc gcagcaggta gcgctgtttg
    10021 aggatctggt ctgcgcaatg atgttgcaca ttcgccaaca ggcgcaggcg cagctgccgg
    10081 aggcaattac tcaggcggtg attggtcgcc cgatcaactt ccaggggctg ggcggtgatg
    10141 aagcaaacac ccaggcgcaa gggattctgg aacgcgcggc gaagcgtgcc gggtttaagg
    10201 atgtggtatt ccagtacgag ccggtcgcgg ctgggctgga ttacgaagcc accttgcagg
    10261 aagaaaaacg agtgctggta gtggatatcg gcggtggtac gactgactgt tcattgctgc
    10321 tgatggggcc gcaatggcgt tcgcgtctcg atcgtgaagc cagcctgctg ggtcatagtg
    10381 gttgccgtat tggtggtaac gatctggata tcgcactggc gtttaagaac ctgatgccgt
    10441 tactgggcat gggtggcgaa accgaaaaag gcatcgccct gccgattctg ccgtggtgga
    10501 atgcggttgc gatcaacgac gttcctgcgc aaagtgattt ctacagtagt gctaactgtc
    10561 gtctgcttaa cgatctggta cgcgatgccc gtgaaccgga aaaagtggcc ctgttacaga
    10621 aagtctggcg tcagcgttta agctatcgcc tggtgcgtag cgcagaagag agcaaaatcg
    10681 ccctttcaag tgtagcggaa acccgcgcct cactgccgtt tatcagcgat gaactggcta
    10741 cgctgattag ccagcaaggg ctggaaagcg ccctcagcca gccactggcg cggattctgg
    10801 aacaggtgca actggcgctg gataacgccc aggaaaaacc ggacgttatc tacctgaccg
    10861 gcggtagcgc ccgttcgccg ctgattaaaa aagcgctggc agaacagttg ccgggcattc
    10921 cgattgcagg cggcgatgac tttggctccg tcaccgccgg gctggcacgc tgggcggaag
    10981 tggtgtttcg ttaattgaaa aatggctcag caacggctta aatctgcctg cgcctgagcc
    11041 agtttttctg ccgcctgacg taacggttcc atcaacggca tggtccgtgc tggagcttga
    11101 aggcggaatc gctgcaactc tgtcgcaccg acagtcagcg ccgattccag ccgatgtcgt
    11161 ttggcggcca tttttgccat taccgcttgt ctgtctgcag gcgttaccgc ttcgttcggc
    11221 ctgaaaacaa aacggcgttc gcaactcgct ttccagtcga taaccgcctg ggtcagatga
    11281 tcaccaaaac ccctaacttg cttaacgctc cgtcgggtaa cgtccgctgc tgtttcaata
    11341 ccaaaggacc gtaacgccgc tttacgcgca gggccaacgc cgggaataga ggcaacatca
    11401 ataaaaaatc cctccagaaa cttctgcttc tgccgttccc ttgcggtgtc gtgaagtgct
    11461 gccagagcgc gtttctcttc ttccggtaac ccgagaattt cgtccttcat tttttcgagc
    11521 atcgcccgtt tggcgataaa accttccagc ccgcccaact gctggatctg gctgactaaa
    11581 tggtcatagt ccattttcgc acgattaaat gcctcacggc gttgctgtat ttctgctttg
    11641 tacgctttac ttgtcaggct gccaataatc cagatagccg ccagcacagg aatcaaaata
    11701 atataacgcg gttctgcctg aaggccgcaa agcaacagcg ataacgctga cagtgcgatc
    11761 tcaatcagaa tgatgtattc gcgccgtaac aggcctgaag gaagcagcct gccagtcggt
    11821 tggaaatgat cgggtaatgg cagttgcaat gctggcggtg ctactgatgc catcaccatc
    11881 gcccagactc tcgccagcac aaaatcaccg ccggtggtaa tgacctcttc gctgagatca
    11941 ataaaataga taacgccttg attatccagc gcacaccacg ggcagtcagc caaatgagcg
    12001 gggtaaacat gcattgccga aacggtacat ttctttaact gttggcgtag taaatcaagt
    12061 gccgctaccc acgcttttgc cgtcggacgc ccggttgcca cgccactttc cgtgaatgcc
    12121 tgctgaaaca tggcttcaac atcgcccggt aacatcgata gcggaatcga tcgtggcggc
    12181 ggttttaaac cacgtcgctg attatctgac gcgtaggcat aacggaaatg ggcaatatcc
    12241 atctccagcg cattacccgc atcagagata agcggtacac cggaataagg atgccgccca
    12301 ccaaacaaga tgtgaaatat cagcaacgca aggccaaaat tatcgtgatt ttctgtgcgt
    12361 tcaaagccga caaatgatgg catggtttgc agctctggcg gcgtaaaatg cgacacgccg
    12421 acttcgcata aatgcagtgt accattggca ttaatctgaa aggagtcgct atcaatcaac
    12481 accactttgc tgtcgcgacc taccataaag ctgttctggt ttacgtcacc cacgacgtgc
    12541 ccgtgctcat gaacagtagc aaaagatgaa gcaatattgc gcgcaacata gagtagaaaa
    12601 tcccacgcac aatgagggta actctggcga cgatgtgccg ggctatagat catatgaatc
    12661 ggttctttac cagaaacttt tggcatcata aaaccgataa cttttccgcc tcgtccaccg
    12721 tgaagcgttg cctgcggcca ggcgacataa ttcaacaact gcgcgtcagc tgtcgcagcc
    12781 ataaaggcaa gtttgtcctg ttttaaggcg ggtggcggcg tgtgataaat cttggcgacg
    12841 ctatcgacaa actcctcgat atcataaacc gcaccttcgc cgcctttgcc cagttcacgg
    12901 cctggcgtaa cgcactcacc agtagcagta tataaagtgg gtttcatggt aaccacatag
    12961 ccagtgccaa cgttttatca tcatccgtac gctcgtttac cgccgggctg gacaaaaaca
    13021 gctttaacaa ttcaggtaat aaatcgagtt gttcctgcgt tgctgacgcc agtccattga
    13081 aaaacggggt aaagaaaggc acatgcggag aattatccag catatttaac gccagtcgct
    13141 gaataccatc cgtaaatgct gcaactttat gcgcacgctc agtgctggtg aaagtttcca
    13201 gtctggaaac tgcatcttca tcggtaatga agtgcgtcat attggcatat tcacccacca
    13261 tcggcgtgag cggcagttgc aaaccgtgac ccaaatctac caccacgccg ccatcgccaa
    13321 tctgcataat caaggtgcca ttagctgatg aaatcaatcc caaaaacgtg caggcaaaat
    13381 cgcgaatcgc taattctttg gcttccgcct cggcaaaaag tcgctgacga atggtcagca
    13441 caatgtccgt tgcgaggaca tcattaagcc ccaattcccc gccctgcact ttttgcgaca
    13501 tataagccat cgcttcattg acggcgagca tcgcaccttc gccaccctgc gagacgctgc
    13561 ctgcgccatc agcgacaaat accgacaata atggctgctg gtcatttagc caggcaacct
    13621 gcatctggca ggcatcctgg caaggcaaat cagcgctaat atgcgacgtt ccaacggctg
    13681 atgcatagac cagtcgccag ctcacactga cgtccagcct tttggcgctt ccagcacaac
    13741 ttccgttccc ggcgtggagc gggaaaccga acgcagcgaa ctggataacc agctaaacag
    13801 ttcacggaat tgtaatccct gcagagacaa aggctgacgg acgctgattt gggccagcgt
    13861 cttcatatcc gccccctgaa caccgatggt aaagaaggca aacttcttat cttcttcccc
    13921 ctgaaacact ttgttggcgg cagcctgcca ctcatcggtt ggtgcgccat cggtgatcag
    13981 gaaaatccac ggacgataat aggaaatacc attggcacga tattcacgtt tccgctcctc
    14041 aaccatatcc agggctttgg taatggcagc gcccattggt gtatcgccct gggcaaacag
    14101 gatgggcggg aaaaaattag cggcgctggt aaagggttgt tccacatgca ccgggccgaa
    14161 cgtcacaatg ccgagttcca ctctttttaa ggccagcgaa tcggcaagca gctcatcgcg
    14221 aaaggtaacc aatccggtgt taagttcatt aatgggtctg ccgctcatcg agccagaaac
    14281 atccagcagc aaaatgcagg ggcaacgtgg ttccgggtta ctggcaaaat cgctggtggc
    14341 aaacgtgatt tgttcgctca taagaaatcc tttcttagag aattattgag gtccggtatc
    14401 ataccgattg caataatgaa ataagagcaa acgaagaaac tttcagaatt aggcccggcg
    14461 gcgcagaaaa ttttgctatt cgcctcgcaa gaacgtgggc ttacagattg acaacaaggg
    14521 ttgagtgaca taatttcagg agtgagggta gagcggggtt tcccccgccc tggtagtctt
    14581 agtatgctgg gtagctaatg actaagagca ccacgatgat gagtagcttc atcatgaccc
    14641 tttccttatt taaggcccct tcctcgggag gggctttccc gtttcagcgt cccgctgaaa
    14701 tcgtcggctt acctcctttc gccctgtatg cagtctatcg ctgacttgcg gataaaatag
    14761 tttcaagtgt gattattgcg taacgactcg caaagaatat ttgctggcag gatcgcagac
    14821 tgcaaagtct gcggattgac aattggaccg tgaaggcata ctttcaggaa tgagggttga
    14881 gcggggtttc ccccgcccta gtagtcttag taagccggga agcttatgac taagagcacc
    14941 acgatgatga tccacttcat catgaccctt tccttattta tggccccttc ctcgggaggg
    15001 gctttcccgt ttcagcgtcc cgctgaaatc gtcggcttat ctcctttcgc catgcaagca
    15061 gtctatcgct aacgcgtaga taaaatagtt tcatgtgtta ttactggatg cgtgctcgca
    15121 aaagtgcccg tcattcagac gattcccgac agtgtttcat aattcctcca tttttctccc
    15181 ttattggctg gctacactag tatcattccg cgaaacgttt caggaagaga aactcttaac
    15241 gatgaaaggc agttataaat cccgttgggt aatcgtaatc gtggtggtta tcgccgccat
    15301 cgccgcattc tggttctggc aaggccgcaa tgactcccag agtgcaaccc caggggcgac
    15361 gaaacaagcg cagcaatcgc cagcgggtgg tcgccgtggt atgcgtgccg gcccattagc
    15421 tccggttcag gcggcgaccg cagtagaaca ggctgttccg cgttacctca ccgggcttgg
    15481 caccattacc gccgctaata ctgttacggt gcgcagccgc gtggacggtc aattgatggc
    15541 gttacatttc caggaaggcc agcaggtcaa agcaggcgat ttactggcag aaattgaccc
    15601 aagccagttc aaagttgcat tagcacaagc ccagggccaa ctggcaaaag ataaagccac
    15661 gctcgccaac gcccgccgtg acctggcgcg ttatcaacaa ctggcaaaaa ccaatctcgt
    15721 atctcgtcag gaactggatg cccaacaagc gctggtcagt gaaaccgaag gtaccattaa
    15781 ggctgatgaa gcaagcgtcg ccagcgcgca attgcaactc gactggagcc gtatcaccgc
    15841 accagttgat ggtcgcgttg gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg
    15901 cgacaccacc gggattgtgg tgatcaccca aacacatcct atcgatctgg tctttactct
    15961 gccggaaagc gatatcgcca ccgttgtaca ggcacaaaaa gccggaaaac cgctggtggt
    16021 agaagcctgg gatcgcacca actcgaagaa gttaagtgaa ggcacgctgt taagccttga
    16081 taaccaaatc gatgccacta ccggtacgat taaagtgaaa gcacgcttta ataatcagga
    16141 tgatgcgctg tttcccaatc agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa
    16201 cgccgtggtg atccccaccg ccgctctgca aatgggcaac gaaggccatt ttgtctgggt
    16261 actgaatagc gaaaacaaag tcagcaaaca tctggtgaca ccgggcattc aggacagtca
    16321 gaaagtggtg atccgcgcag gtatttctgc gggcgaccgc gtagtgacgg acggaattga
    16381 tcgcctgacc gaaggggcga aagtggaagt ggtggaagcc cagagcgcca ctactccgga
    16441 agagaaagcc accagccgcg aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta
    16501 cccccgagca gcacaggcgg cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg
    16561 ctgctgatgg tggcgatctt actcgccggg attatcggtt atcgcgccct gcccgtttcg
    16621 gcgctgccgg aagtggacta tccaaccatt caggtggtca cgctttatcc aggtgcgagc
    16681 ccggatgtca tgacctctgc cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct
    16741 ggcctgaaac agatgtcgtc gcaaagttcc ggcggtgcgt cagttatcac tttacagttc
    16801 cagctaacat tgccgctgga tgtcgccgag caggaagtgc aggccgcgat taacgctgcg
    16861 accaacctgc taccgagcga tctgcctaac ccgccggttt acagcaaagt gaacccggca
    16921 gatccaccga tcatgacgct cgctgtcacc tcaaccgcca tgccgatgac gcaggtggaa
    16981 gatatggtgg aaacccgcgt cgcgcagaaa atctcgcaga tttccggcgt agggctggtg
    17041 acactttccg gcgggcagcg tccggcggta cgcgtaaaac tcaacgctca ggcaattgcc
    17101 gctctcggcc tgaccagcga aagtgtgcgc accgctatta ctggcgcgaa cgttaactca
    17161 gcgaaaggga gccttgacgg cccttctcgc gcggtcacgc tttccgcgaa cgaccagatg
    17221 caatcggcag aagagtatcg tcagctaatc atcgcctacc agaacggcgc accaattcgt
    17281 cttggcgatg tcgctactgt agagcaagga gcagaaaaca gctggctcgg cgcgtgggcg
    17341 aacaaagaac aggccattgt gatgaatgtt cagcgccagc ccggtgctaa cattatctcc
    17401 accgccgaca gcatccggca gatgctacca cagctcaccg agagtctgcc gaaatcggtg
    17461 aaggtgacgg tactttctga ccgtaccacc aatatccgcg catcggtcga cgatacccag
    17521 tttgagctga tgatggctat cgcgctggta gtcatgatta tctacctgtt tttgcgcaat
    17581 attccggcga ccatcattcc cggcgtcgca gtgccgctgt cgttaatcgg caccttcgcg
    17641 gtcatggtgt ttctcgattt ttcaatcaat aacctgacgc tgatggcact aaccatcgcc
    17701 accggatttg tggtcgatga cgccatcgtg gtgatcgaga acatttcccg ctatatcgaa
    17761 aaaggcgaaa aaccgttggc ggcggcgctc aaaggcgcgg gtgaaatcgg ctttaccatt
    17821 atctcgctga ccttctcgct gattgcggtg ttgatccccc tgctgtttat gggcgatatc
    17881 gtcgggcgac tgttccgcga atttgctatt accctggcag tcgcgatttt gatctcggcg
    17941 gtggtgtcgc tgaccctgac accgatgatg tgcgcgcgga tgctcagcca ggagtcgctg
    18001 cgtaaacaga accgcttctc ccgtgcctcg gagaaaatgt tcgacaggat aatcgccgcc
    18061 tatggttgtg gactggcgaa agtgctgaat catccgtggc tgaccttaag cgtggcgctc
    18121 agcacgctgc tgcttagcgt gctgctgtgg gtattcattc cgaaaggttt cttcccggtg
    18181 caggacaacg gcattattca gggcactttg caggcaccgc aatccagctc ctttgcgaat
    18241 atggcccagc ggcaacgcca ggtcgcggac gtgattttgc aggatccggc agtgcaaagt
    18301 ttgacctcat ttgttggcgt tgatggaact aacccgtcgc tgaacagtgc gcgtttacag
    18361 atcaatctca aaccactgga tgaacgagat gaccgggtgc aaaaagtcat cgcccgtctg
    18421 caaacggcgg tggataaagt gccgggcgtc gatctcttcc tgcaaccaac gcaggacctg
    18481 actattgata ctcaggtcag ccgcacccag taccagttta ccttgcaggc cacgtcactg
    18541 gatgcgctca gtacctgggt gccacagttg atggaaaaac tccagcagct gccacagctt
    18601 tctgatgtct ccagcgactg gcaggacaaa gggctggtgg cgtatgtcaa tgtcgacagg
    18661 gacagcgcca gccgcctggg gatcagcatg gcggatgtcg ataacgccct gtacaacgcg
    18721 tttggtcagc gactgatttc cactatttat actcaggcta accagtatcg cgtggtactg
    18781 gaacacaaca ccgaaaatac cccaggcctc gcggcgctgg ataccattcg cctgaccagc
    18841 agcgacggtg gcgtggtgcc gttaagctca attgccaaaa ttgagcagcg ttttgcgccg
    18901 ctttccatca accatctgga tcagttcccg gtaacgacta tctcctttaa cgtgccggat
    18961 aactattcgc tgggcgatgc ggtgcaggcg attatggaca cagagaagac gctgaatctg
    19021 ccggtggata tcaccacgca gttccagggc agcaccctcg ccttccagtc ggcgctgggc
    19081 agcacggtct ggctgattgt cgcggcggtg gtggcgatgt atatcgtgct cggcattctg
    19141 tacgagagct ttattcaccc gatcaccatt ctctcgacgc tacccaccgc aggggtcggc
    19201 gcgctgctgg cgttaatgat tgccggtagc gaactggatg tgattgccat tatcggcatt
    19261 attttgctga tcggtatcgt gaagaagaac gctatcatga tgatcgactt cgcgctggct
    19321 gctgagcgcg agcaaggcat gtcgccgcgc gaagctattt accaggcttg tctgttgcgt
    19381 tttcgtccga tcctgatgac cactctggcg gctctgcttg gtgcgctgcc gctgatgttg
    19441 agtaccgggg tcggcgcgga actgcgtcgt ccgttaggta tcggcatggt cggcggtctg
    19501 attgtcagcc aggtgctgac gctgtttacc acgccggtga tttatctgct gttcgaccgt
    19561 ctggcattgt ggaccaaaag ccgctttgcc cgtcatgaag aggaggcgta agtgaagttt
    19621 tttgccctct tcatttaccg cccggtggcg acgattttac tgtcggtcgc cattaccctg
    19681 tgcggcatac tgggtttccg tatgctgcca gtcgccccgc tgccgcaggt cgattttccg
    19741 gtgatcatgg tcagcgcctc gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt
    19801 gccacgccgc tggagcgctc acttgggcgc attgccggag tcagcgaaat gacctccagc
    19861 agttcgctcg gcagcacgcg cattattttg cagtttgatt ttgaccggga tatcaacggc
    19921 gcagcgcgcg atgtgcaggc ggcgatcaac gccgcgcaaa gtctgctacc cagcgggatg
    19981 cccagccgtc cgacctatcg caaagcgaac ccgtcggatg cgccaattat gatcctcacg
    20041 ctgacatccg atacttattc gcagggtgaa ctgtacgatt tcgcctcgac acagctggct
    20101 ccgacgattt cgcaaatcga cggtgtcggt gatgtcgatg ttggcggcag ctcgctgccc
    20161 gccgtacgcg tcgggctgaa cccgcaagcg ctgtttaatc agggcgtgtc gctggacgat
    20221 gtgcgcaccg ccatcagcaa tgccaacgtg cgtaaaccgc agggggcgct ggaagatgat
    20281 actcaccgct ggcagatcca gactaacgat gaactgaaaa ccgccgctga atatcagccg
    20341 ctgattattc attacaacaa cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc
    20401 gattcggtgc aggatgtgcg caacgccggg atgaccaacg ccaaaccggc tattctgctg
    20461 atgatccgca aactgccgga agccaatatt atccagacgg tagacagcat ccgcgcccgt
    20521 ctgccggaat tacaatccac catcccggca gcaattgatc tgcaaattgc ccaggatcgc
    20581 tcccctacta tccgcgcgtc gctggaagag gttgagcaga cgctgattat ctcggtggcg
    20641 ctggtgattc tggtggtgtt tttgttcctg cgttcgggtc gcgccaccat tattcccgcc
    20701 gttgcggtgc cggtttcgct gattggtacg tttgcggcga tgtacctgtg tggtttcagt
    20761 ctcaataatc tttcgttaat ggcgctcacc atcgccactg gctttgtggt ggatgacgcc
    20821 atcgtggtgc tggaaaacat tgcccgtcat ctggaagcgg ggatgaaacc gctacaggcc
    20881 gcgctgcaag gtactcgcga agtcggcttt acggtgctgt cgatgagtct gtcactggtg
    20941 gcggtgttcc tgccgctgct gttgatgggc ggattgccgg gtcgcctgtt acgcgagttt
    21001 gccgtgacgc tttctgtcgc cattggtatt tcgctgctgg tttccctgac gttaacgccg
    21061 atgatgtgcg gctggatgct aaaagccagc aagccgcgcg agcaaaagcg actgcgtggc
    21121 tttggtcgca tgttggttgc tctgcaacaa ggctacggca agtcgctaaa atgggtgctc
    21181 aatcataccc gtctggtggg cgcggtgctg cttggcacca ttgcgctgaa tatctggctg
    21241 tatatctcga tcccgaaaac cttcttcccg gagcaggaca ccggcgtact gatgggcggg
    21301 atacaggctg accagagtat ttcgtttcag gcgatgcgcg gtaagttgca ggacttcatg
    21361 aaaattatcc gtgacgatcc ggcggtggat aatgtcaccg gctttaccgg cggttcacgg
    21421 gtgaacagtg gaatgatgtt tatcaccctt aaaccacgcg gcgagcggag cgaaacggcc
    21481 caacagatta tcgatcgctt gcgtaaaaaa ctggcgaaag aaccgggagc gaatctgttc
    21541 ctgatggcgg tacaggatat tcgcgttggc ggacgtcagg ctaacgccag ctaccagtac
    21601 acattattgt ccgacgatct ggcggcactg cgcgaatggg agccaaaaat ccgcaaaaaa
    21661 ctggcgacat tgccagagct ggctgatgtt aactccgatc aggaagataa tggcgcggag
    21721 atgaacctta tttacgatcg cgacactatg gcgcgattgg gaattgacgt gcaagccgcc
    21781 aacagcctgt taaataacgc cttcggtcag cggcaaatct cgaccattta ccagccgatg
    21841 aaccagtaca aagtggtgat ggaagtggat ccgcgctata cccaggacat cagtgcactg
    21901 gaaaaaatgt tcgttatcaa taacgaagga aaagcgatcc cgctgtcgta tttcgctaaa
    21961 tggcaaccgg cgaacgctcc actgtcggtg aatcatcagg gattatcggc ggcctcgacc
    22021 atttcgttca acctgccgac cgggaaatcg ctctcggacg ccagtgcggc gatcgatcgc
    22081 gcaatgaccc agcttggtgt gccttcgacg gtgcgcggca gttttgccgg aacagcgcaa
    22141 gtgttccagg agacgatgaa ctcacaggtg atcctgatta tcgccgccat cgccacggta
    22201 tatatcgtac tgggtatcct ttacgagagt tatgtgcatc cactgacgat tctctccacc
    22261 ctaccctcgg cgggcgtcgg agcgctgttg gcgctggagc tgttcaatgc cccgttcagc
    22321 ctaattgcgc tgatagggat catgttatta atcggcatcg tgaagaaaaa cgccattatg
    22381 atggtcgatt ttgcgcttga agcccaacgg cacggtaacc tgacgccgca ggaagctatt
    22441 ttccaggcct gtctgctgcg ttttcgcccg attatgatga ctaccctggc ggcgctgttt
    22501 ggtgcgctac cgctggtatt gtcgggcggt gacggctcgg agttgcgcca accattgggg
    22561 atcaccattg tcggcggtct ggtcatgagc cagcttctga cgctgtatac cacgccggtg
    22621 gtgtatctct ttttcgaccg tctgcgactg cgtttttcgc gtaaacctaa acaagcggta
    22681 accgagtaaa tgacagatct tcccgacagc acccgctggc aattgtggat tgtggctttc
    22741 ggcttcttta tgcagtcgct ggacaccacc atcgttaaca ccgcgcttcc ctcaatggcg
    22801 caaagcctcg gggaaagtcc gttgcatatg cacatggtca ttgtctctta tgtactgacc
    22861 gtggcggtga tgctgcccgc cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt
    22921 ttctttaccg ccatcgtgct gtttactctc ggttcactgt tttgcgcgct ttccggcacg
    22981 ctgaacgaac tgttgctggc acgcgcgtta cagggcgttg gcggcgcgat gatggtgccg
    23041 gtcggcaggt tgacggtgat gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc
    23101 tttgtcacgc tgcccggtca gatcgggccg ctgcttggcc cggcgctcgg cggtctgctg
    23161 gtggagtacg catcgtggca ctggatcttt ttgatcaaca ttccggtggg gattattggc
    23221 gcgatcgcca cactgatgtt aatgccgaac tacaccatgc agacgcggcg ctttgatctc
    23281 tccggctttc tgttgctggc ggttggcatg gcggtcttaa ccctggcgct ggacggcagt
    23341 aaaggtacag gtttctcgcc gctggcgatt gctggcctgg tcgcagttgg cgtggtagca
    23401 ctggtgcttt atctgctgca cgcccaaaat aacaaccgcg ccctgttcag tctgaaactg
    23461 ttccgcactc gtaccttttc gctgggcctg gcggggagct ttgccggacg tattggcagt
    23521 ggcatgttgc cctttatgac accggttttc ctgcaaattg gtctcggttt ctcgccgttt
    23581 catgccggac tgatgatgat cccgatggta ctcggcagca tgggaatgaa gcgaattgtg
    23641 gtacaggtag tgaatcgctt tggttatcgt tgggtactgg tggcgaccac gctgggcctg
    23701 tcgctggtca ccctgttgtt tatgactacc gctctgctgg gctggtacta cgttttgccg
    23761 ttcgtcctgt ttttacaagg aatggtcaac tcgacgcgtt tctcctccat gaacaccctg
    23821 acgctgaaag atctcccgga caacctggcg agcagcggca acagtttgct gtcgatgatt
    23881 atgcaattgt cgatgagtat tggcgtcacc atcgccgggc tgttgctggg gctttttggt
    23941 tcgcagcatg tcagcgtcga cagcggcacc acacaaaccg tctttatgta cacctggctt
    24001 agcatggcgt ttatcatcgc ccttccggcg tttgtctttg ccagagtgcc gagcgatacg
    24061 catcaaaatg tggctatttc acggcgaaaa aggagcgcgc aatgaagttc tggcgacccg
    24121 gtattaccgg caaactgttt ctggcgattt tcgccacctg cattgtctta ttgatcacga
    24181 tgcactgggc ggtacgtatc agttttgagc gcggctttat cgattacatc aagcatggta
    24241 atgaacaacg gctgcaaatg ctcggcgatg cgcttggtga gcagtacgcc cagcacggga
    24301 actggcgctt cctgcgtaat aacgatcgct ttgtatttca gatcctacgt tcactggagc
    24361 atgataacaa cgaagataag cccggccccg gtatgccacc acacggctgg cgcacgcaat
    24421 tttgggtggt tgatcaaaat aataaagtgc tggttggccc gcgagcaccg gttccacccg
    24481 acggcacacg gcggcccatt atggtcaatg gtgcggaagt tggcgcggtg atcgcctccc
    24541 ctgttgaacg actgacccgt aatactgata tcaattttga cagacaacag cggcaaacca
    24601 gttggctgat tgtggcttta tctaccttat tagcggcgct ggcgacattc ccactggcgc
    24661 gcggtttgct ggctccggta aaacgactgg tggatggcac gcacaaactg gcagcgggtg
    24721 atttcactac tcgcgtagcc cccaccagcg aagatgagtt gggcagactg gcagaggatt
    24781 tcaatcaact cgccagcaca ctggagaaaa accagcagat gcgccgcgat tttatggccg
    24841 atatttctca tgaactgcgc acgcctttag cagtactgcg cggcgaactg gaagccattc
    24901 aggatggtgt gcgtaaattc acgccggaga cgctggcttc tttacaggca gaagtcggaa
    24961 cactgacaaa actggttgac gatctccatc aattgtctat gtctgatgaa ggcgctctcg
    25021 cctatcaaaa agcaccggta gatttgatcc cactgctgga agtggcggga ggcgcatttc
    25081 gcgaacgatt cgccagccgt ggcctgaaat tgcaattttc cctgccagac agtattaccg
    25141 tatttggcga tcgcgatcgt ttaatgcagt tgttcaataa cttactggaa aacagcctgc
    25201 gctacactga cagcggcggc agcctgaaaa tctctgccga acagcacgac aaaacggtgc
    25261 gcctgacctt tgccgacagt gcgccgggtg tcagtgacga tcagctacaa aaattgtttg
    25321 aacgttttta tcgcaccgaa ggttcccgca accgcgccag cggtggttcc gggctggggc
    25381 tggcgatttg cctgaacatt gttgaagcac ataacggccg cattattgcc gcccattcgc
    25441 cttttggcgg gcttagcatt acagtagagt taccgctgga acgggattta cagagagaag
    25501 tatgaccgag ttaccaatcg acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc
    25561 gaagctgggg cagttgctca ttgattatct gcgtgctgcg agctatgcgc caacgttgat
    25621 tagccacggc gatcaggtac tgccatatgt gcgccagaca ccaccggatc tgatcctgtt
    25681 ggatctgatg ctccctggca ccgatggcct gacgctgtgc cgggaaattc gtcgtttttc
    25741 tgacattccg atcgtgatgg tgacggcaaa aatcgaagag atcgaccgcc tgctggggtt
    25801 ggagattggc gcagatgact atatctgcaa gccgtacagc ccacgggaag tggtagcgcg
    25861 cgtcaaaacc attttgcgcc gttgcaaacc gcagcgtgag ttgcagcaac aggatgctga
    25921 aagcccgttg attatcgacg aaggtcgttt tcaggcttcc tggcgcggta aaatgcttga
    25981 cctgacgcct gcagaatttc gtctgctgaa aacgctctct catgaaccag gaaaagtgtt
    26041 ctcccgcgag cagttgctga accatcttta tgacgactac cgcgtggtaa ccgaccgtac
    26101 catcgacagc cacattaaaa acctgcgccg caagctggaa tctctcgacg ccgaacagtc
    26161 atttatccgc gccgtttacg gcgtcggtta ccgctgggaa gccgacgcct gccgcatcgt
    26221 ttaacatttt tacctgccgg agatggcctt cagaccagat ccggcaacat tatcccacgc
    26281 atggtcagca aactgacatt atctctccgc cctggcaggc accagaaaat cgctcatact
    26341 ttaattggca aacagtacct ttagatgctg ttttcgatac gcaatttcaa tcaaggagtc
    26401 attatggctg gttggtttga actcagtaag agcagtgata atcagttccg gtttgtgcta
    26461 aaagcgggca atggtgagac tatcctcacc agcgagctgt atacctcaaa agcctctgcg
    26521 gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa
    26581 acggcaagca atggcaaatt ctatttcaat ctgaaagccg ccaatcatca aattatcggc
    26641 tccagccaga tgtatgccac cgcgcaatct cgtgaaaccg gaatagcctc cgttaaagca
    26701 aatggcacaa gccagacggt gaaagacaat acgtaattac gatgccgggc gtaggttgtt
    26761 ggcgtccggc gctttaccag ttacccccac agcgctacaa tgcccgccct taaagtgggg
    26821 gcactcccct aaccgcttca tcaggtgaag cggctctgac ctgtcatcag aacgagagaa
    26881 ttatgtttaa accggaactc ctttccccgg cgggaacgct gaaaaatatg cgttacgctt
    26941 tcgcttatgg cgcagatgct gtttatgcgg gccagccgcg ttactccctg cgtgtacgca
    27001 acaacgaatt caaccacgaa aatcttcagc tcggcatcaa tgaagcccac gcgctgggga
    27061 aaaagtttta tgtcgtggtc aacattgcac cgcacaacgc caagctgaaa acctttatcc
    27121 gtgacctgaa accggtggtg gaaatggggc cggatgcgct gattatgtcc gatccagggc
    27181 tgattatgct ggtgcgtgag cacttcccgg aaatgccaat ccacctctcg gtacaggcta
    27241 acgccgtaaa ctgggcgacg gtgaaattct ggcagcaaat gggtctgacc cgcgtgatcc
    27301 tctctcgcga actgtcactg gaagagattg aagagatccg caatcaggtg ccggatatgg
    27361 agatcgaaat cttcgttcac ggcgcgctgt gcatggccta ctccggtcgc tgcctgctct
    27421 ctggctatat caacaagcgc gacccgaacc agggcacctg caccaacgcc tgccgctggg
    27481 agtataacgt ccaggaaggg aaagaagatg acgtcggcaa catcgtacac aagaacgagc
    27541 cgattccggt gcaaaatgtt gagccgacgc tgggtatcgg cgcgccaacc gacaaagtgt
    27601 ttatgatcga agaagcccag cgtccgggcg agtatatgac cgcgtttgaa gatgagcacg
    27661 gcacttacat catgaactcg aaagatctgc gcgccatcgc ccatgttgaa cgcctgacca
    27721 aaatgggcgt gcattcgctg aaaatcgaag gccgtaccaa atctttctac tattgcgcac
    27781 gcaccgcgca ggtttaccgt aaagctatcg atgacgccgc tgcgggcaaa ccgttcgata
    27841 ccagcctgct ggaaactctg gaaggtctgg cgcatcgtgg ctataccgaa ggtttcctgc
    27901 gtcgtcatac ccacgacgat tatcagaact acgaatacgg ttattcggtt tctgaccgtc
    27961 agcagtttgt tggtgagttt accggtgagc gcaaggggga gctcgcggcg gtagcggtga
    28021 aaaataaatt ctccgttggc gacagccttg agctgatgac gccgcaaggc aacattaact
    28081 ttacccttga gcacatggaa aacgccaaag gtgaagcaat gccggtcgca ccaggcgatg
    28141 gttatactgt gtggctcccg gtgccgcagg atcttgagct aaattacgcg ctgctgatgc
    28201 gtaatttctc cggggaaacc acgcgtaacc cccacggtaa gtgattaatt tcgattattt
    28261 ttcccggatg gaaaattctt agaaaccgat cacatacagc tgcatttatt aaggttatca
    28321 tccgtttcgc tgaaaaacat aacccataaa atgctagctg taccaggaac cacctcctta
    28381 gcctgtgtaa tctcccttac acgggcttat tttttatgca taagccctat ccctggtcac
    28441 cgtcttccat tgaccacatc gatagaatcc tccttcatag cacgatgcct ttcacttatc
    28501 gacatcgtgc tcgcacaggt tccggttacg cacagccaga acgcgcatgt ttgacgctta
    28561 ccaaaaaatg aacctaaagc attggaatat ttttgacatc attttctgat ggctgcataa
    28621 aataaaaatt ctgctttagt ttcatctatc tgtttgtcat taatactcac attcaataat
    28681 agtgttgaag aatatcccat cacagataaa ataaatatgt gcatggtagt cttgaatcta
    28741 ttctcactct ccacatttga atgtcagacg agcgacgcca tgtaatcctg caccttctgt
    28801 cttcaggtca actatctgca tttttttgcc ctgagtaaca cagaaatgag ttgcatcatt
    28861 ttttactata ttttctgcac cagatattct acccctggct aaagaagctt cggcttcggt
    28921 gtagtattgg ttatcgagtt tacgctgaat attactttta tatgcaaggc caaatttacc
    28981 gatacttgtc tcatcattat gcacagcaca accagacata agaaaaacac taattaatga
    29041 tatagcagct atctttttca ccttaccttc ccccattaat taccaacgac attctctagt
    29101 gtttaaatat aataatggca tgattattat aattgaatag gattataata aatgttctgt
    29161 acagcatttc ctacataagt aggaattaca gacatggagg cccttccggg caaccccatg
    29221 ggggacttta atatattttg aatagtatat tgaagatgcc actgtttagt tgaatattag
    29281 gtatattctc ttttttaaaa tttatctgtg gctaccgtta atattcgctg tccccattgt
    29341 aagctcctgg tggtaagcac tgaatcccca tacctgaact gactttttat cctccgactt
    29401 tcatcctgtt ctgactccac cttttctttt ctgctctaca ctatctacag accaatcata
    29461 aaggcacata cgatcatggc agaatttccc gccagcttac tgattcttaa tggcaaaagt
    29521 actgacaatc tacccttgcg cgaagcaatt atgctgttgc gtgaggaagg aatgacgatc
    29581 catgtgcggg ttacctggga gaaaggtgat gccgtacgat ttgtagagga ggcccggaag
    29641 ttaggtgtcg caacagtgat tgccggtggt ggcgatggca ccattaatga agtttctacg
    29701 gcattgattc aatgtgaggg ggatgaaata cccgcgctgg gaattttacc attaggaact
    29761 gccaatgatt ttgccactag tgtagggatt cctgaggcac tggataaggc gctgaaactg
    29821 gcaattgccg gtaacgccat tgcgatagat atggcgcaga tcaacaaaca aacctgtttt
    29881 attaatatgg cgacaggcgg gtttgggacg cgtattacca cagaaacgcc ggaaaaatta
    29941 aaagccgcgc tgggtggcgt ctcttacatc attcatggct taatgcgcat ggacactctg
    30001 caaccggacc gttgtgaaat ccgcggtgaa aactttcact ggcaaggtga cgccctggtc
    30061 attggtattg gtaacgggcg tcaggccggt ggcggtcaac aattgtgccc gaacgcatta
    30121 attaacgatg gcttgctgca actgcgcatt attaccggcg atgaaattat tccgactctc
    30181 gtatcaacct taaaatctga cgaagataac ccgaatatta tcgaaggagc ttcgtcgtgg
    30241 tttgatatac aagccccaca cgaaatcact tttaatcttg atggcgaacc gttgagtggg
    30301 caaaacttcc atattgaaat acttccggcg gcgttgcgtt gtcgattacc accagattgt
    30361 ccattgttgc gttaatcaaa ctattctctg ttgaaccaga gagaatccgt aatgccggat
    30421 acggtgtaaa caccttatcc ggcatacaga acaatactac accgtaatta attttacccg
    30481 tgtcttctgc aatgccagtt tatccacccc agacacagca tcatcggtaa taataatgtc
    30541 aattttttcg attggcagta cctgattaaa accgcgacga ttaaatttcg atgaatcaag
    30601 aacggcaatc actttattcg ccgctgtcac catcgcaccg ctaacggaat aaccttcatt
    30661 aaaggtcgta ataccattaa ccgcatcaat accatcagca ccgacaaaca ttaaatcggc
    30721 attaatatcc tgcagggaac gttcagcaat agatccatgc atagatcgcg ttttatggcg
    30781 gaccgtgcca ccgcagacga ccagagtaat gtctttattt tcggaaaggg caaacgccgc
    30841 cgggagactg ttggtaatca ccgtgatatt tttggcggtc attaatcctt cggcgataag
    30901 cattgtggta cttccgctgt cgagaataac ggtcatccct tcgtggatca ttttgactgc
    30961 cgcctgcgct attctgtttt tcggcgcgct ggcgagctga aatcgctctt taaacccgac
    31021 ttcctgggtc tcggtttcgc tattaccgga cattattttc gccgcaccgc catgaaagcg
    31081 cgtaaccacg cctttttgtt cgagaaagcg caaatcggca cggattgtcg cttccgaggc
    31141 agcaaatact cccgccagat cctgaacaag cacggttccc tgttcattca ctaattggat
    31201 gatcttattc cttcgctcga atgaattcat cgtcatttac ccatattcaa ttgtggctag
    31261 tgtaaacgaa gggggaccat tggtgaatga tctgttttgc acaataccgg gcctgcatca
    31321 cgctggcccg cggtttcagg gaatgagcaa cactttgccc ggcatggcat tacgagcgat
    31381 gtcacgcacc gcctgggcga agctttcaaa gcttccacgg tgagcgatta atggctccag
    31441 gcttaactta cgttctgtca gcaaccggct cgccgtttcc cactcctgcc ccggccaagg
    31501 gctggagtag ttcatccaac tgccgataac cgtcagctct ttacgcaata ttttgccaaa
    31561 cgttgcagat gttaaatgca gatcctgatg caacgtgcct accagcgcca gttgggcatg
    31621 aggaccggca atctctaccg ccagttcgac ggtttgcggg acgccagccg tctcgaggat
    31681 aagctgatta aagcgcagat cgcgtaaaac gccctgtatt tgcggcgcgc tcatttcacg
    31741 gctgttaaat gtttgcatcg caccgaaaga ttttgccagt gccagttttt ctgagctaat
    31801 gtcgatagcc gtcacactct ttgctcccaa cgcgacagcg cactgaattg ccagcaggcc
    31861 aatggttccg gcaccaataa taataacgtt tttattctca catccttgcg ctaaatggaa
    31921 cgcatgcagg ccaacggtaa tcggttcaat aaaagcgcca tcctcaatag gcatatccgc
    31981 aggtagtgca aagacattgt ttcgcttaac gacaatatat tcggcaaatc caccatcacg
    32041 ccgtgagcca ataaaatcat attttgcgca ctgggaataa aaccctttca gacactctgg
    32101 acaagtaaaa caggggaata acggcacaca ggcaaccgca tcgccggggt gtaaatcatc
    32161 aacaccggat cccaccgcat caatatagcc actaaattca tggcctaacg ttattggata
    32221 ataatgtgca ccatttttaa atatcctggg taaatcggaa ccacataagc ccgagctggc
    32281 aatttttacc cgcacctcat cctgatgttt aatttcagga atgacgcttt ctgctacgcg
    32341 cacgatacca tcagtatcat tcaccactga tttcataaaa actcctgatt gttaaggggg
    32401 ataacctccc cctcaggtaa aaattattct gcgagaacgg ctttctcttg tttaataaaa
    32461 ccacgcgctc tacgccaggt catgaaaata ccggtaagat aaattgcgcc gataacaatg
    32521 aaaccgggaa tattttgcgg ggagaaaacc tgaatcagta accaggtaat gggagaaccg
    32581 ccctgatcca ttgaagccac catacccccg gcttttaacg ccccagcatt agccgccagt
    32641 tgggtgtgca aaccaatagt ttgcgtcgcg atccacaggg tgatgctcat aatgatgaca
    32701 cccgagatta aggtgcggaa cagatttcca cgatgcaccg cgaccgccat cgctacaaag
    32761 aaaccgatgg tggcaaggtc gccaaacggc agtacctgat tacccggaac acaaacagca
    32821 attaaaatgg tgagcgggat aaaaatcagg cttgccgata ccaccgccgt atgccccagc
    32881 agcaacgccg gatcaaggcc aatcaggaac tcctgaccgc cgaacttcgc ctgtaaacga
    32941 ctacgggcct gcttagcgat gggcgttaaa ccatccatga tgggtttaat cacccgtggc
    33001 atcagcagca taactgccgc cgtttttacc gccagctgca atacaccttt gacatcgtaa
    33061 cccgcgagga tgccgataat cagccccatc acaaagccca cggtgacagg ttcgccaaat
    33121 gggccaaatt tgcgctgaat atcgtcggcg ctaaatttaa tacggttaac gcctgggatt
    33181 ttctcgatga tagcatccac cagcaccgca attggcccca gatacgccga cgtaccgtgc
    33241 ggaatagcaa tgccttccag ctcaaagaaa tttcgggtat cgcgggcaaa ccagtcgccg
    33301 agcttataaa caaacgccgc gtgaattacc acacccgcca tccctatcat ccatgaaccg
    33361 gttgccagat gcagcaatgc gccggtgaag gtcatatgcc agatattcca gatatcaaca
    33421 tttaccaccc gcgtcatacg ggtcagtagc atcgccacgt taaccagaat cgcaatcgga
    33481 atcgctacca gcgcaatttg cgacgcccag gtcattggtg aagagcccgg ccaaccgaca
    33541 tcgaccacat gcagattcag gtcaaaattt tccgccatcg ctttcgccgc cgggccaatg
    33601 gaatccagca ttaagtcaat cacaaggcca ataccaacaa agccaatccc gatatgcaga
    33661 cctgctttaa agcaatcgcc tgccttcatg cctaatattt tagaaaaaat aatgatgaca
    33721 atcggcagca tcaccgtagg gccgaggtcg agaatataac gcatgacttc tgaaaacata
    33781 ggtcacccct gtaagatagt cagaatttta ttttgtaatg cttcgatacc gacaccagaa
    33841 acaaaaggca tgccgtgaac taacggaata tcgccaaaac ttcgatccac cctggcagtg
    33901 gtgcatatca aatgcacgcc atccatatag gtttctattt cattaactcg acactggatt
    33961 aattcaacag gaatattatg actctgacac aactctttaa tttcttccgc cgccatcgta
    34021 gaggtcgcaa ccgcgcctcc gcaagcgaca ataatcttgc gtttcataaa tagtcctttt
    34081 tattgttaca gagggattta ttcgtctgaa tctaaaacat actttgtgaa gtattccttt
    34141 aactgggttt caggaagagt gattagtgtc tcgacgatct cgggctgttg taacttacca
    34201 aataaacagc gtaaaagttt caattgctgc tgcggatttt ccacaattaa cgcaataacc
    34261 aacgataccg ccacgtcgtt atcatcatcc gcttgctgaa aatgaacttt atttgttggc
    34321 cttaacagat aaatggctga cgacttagca tgaatcgcct cacaatgcgg tattgcaatg
    34381 gcgtgctgct caagcattat cccggtaggg aattctgctt ctctggcaat taatgcctgt
    34441 ggccaggtat catgaaccac acctttcgcg agcatctcat taccgatatg ggttaaaact
    34501 tcgctacgat cgacaaaaga aattccgcta cgaacaaaca ggttagtcat ataccgtcct
    34561 tattccgcac tgccgtagcg ataggcgcgc aaaacatcat aaattttatc cataatcaac
    34621 tgatgcggta ttgctgataa ttccccggac tgaatgcgtt caaattgttt aggaagatac
    34681 tgactaatca tgcccagagg gatttccatg ccttgcagat tcaccatcat cgtttcgacg
    34741 ctatttttaa tccgactatg cggccagtaa taacgaatac gatccgacag gctgtaacga
    34801 atatccagta atgaatcgtt aaaacccgta cgataatatt ttttccagta ttgcggttcg
    34861 tcaaacatca cttcttcaat taccgccaag caaccgctgc gattttctgg agcgatgagt
    34921 tcctgctcaa tttgtgccag cgcaaatatc gcctcacgta aagcaaaggt taatgccgga
    34981 ccgactttca aaattgcaaa atgatcgcga actaattccc gataggccgt ctgagtctgg
    35041 taatcggtgg aatgcgcttc ataaaccatt ttggtttttt ctatccattg cgccagcgcc
    35101 tgagcttctt gcgcctgata atgaataata ttgctatggt caaattccac gcccggctgc
    35161 accacgatgg caatcacgcg tgttaacgcc tctgccagcc cacgggcaat aaaggccttt
    35221 tgatgcgtac gtaaagtatt ggcggcatct tcaacctggg tgatgtgtac tgactgaatg
    35281 gcgctggcct caccgcccgg aaccggaact tcggtgccaa tgacatagct cagttgctca
    35341 cgctggcaat ctgtcgccac actttccgca gcaaagcaaa gcacagcagc tcgtcccgca
    35401 accgtttctg gggctaacgg tataggatcc cccgcgcaag acattgacgc atcaagatga
    35461 attttactga accctgcacg cacgtatgcc tttaccagtt cgacggattt ttccatcgcc
    35521 gcatccgcat tttcttgctg ccagcagttt ggtcccagat ggtcgccacc gagaataata
    35581 cgctcacgag caaatccgac tttatcggca atcgcaaaaa caaattcgcg aaagtctgcc
    35641 ggtgtcattc cggtataacc gccaaattga ttgacctggt ttgacgttgc ttcaatcagc
    35701 actttgcgcg tgctgttgcg atcaaatgcc agcgccgctt cgataaccaa cggatgggca
    35761 gaacagactg aacatatgcc gatatgttca ccagctttat gccgggcaat taacgttttc
    35821 atattttttc cttgttaaat ggcaggtgcg ttatgccctg ccctcgcagc cacaatcggc
    35881 aatcactttg ctcaccacat cgcgcattgc agatttagcc gactgcaaat aatcacgggg
    35941 atcggtcgct tcggggtgct cggtcaggta attttttaac gcctgcgaga aggcattttt
    36001 tagctctgtt gcaacgttga ttttgcatat ccccagtttg atggtttgct gaatatcctt
    36061 agtcgataat cctgacgcgc catgcagcac cagcggtaaa ttcacccact ggcgaatgtt
    36121 ctccagtcga gaaaaatcaa gcgccggtgc tctggcatac ataccgtgag ccgtgccgat
    36181 ggcgaccgcc agggaatcaa tcccggttgc ctcggcaaat tcacgcgcct gaacggggtt
    36241 ggtataaaaa gcatcggctt cattgacttg cacatcatct tcctggccgc caagttgccc
    36301 cagctccgcc tcgacgctga catcaaagcg atggcaaaaa tccaccacct ctttgacccg
    36361 tgaaatattt tgcgcaaaag gcaaatgcga ggcgtcaatc atgactgagc gcacgccaga
    36421 acgaaccttc tgagcgatat cgtcaaattt cgtgtgatgg tcgagatgaa ttgtcagtgg
    36481 atggtgatat tgcttcgcca tcgcgctgac cagcgccaac agattttctg taccagcatg
    36541 agtaaatgtg ccaggcgttc cggcgatgat gaccggcgca tgcaggttgg cagcggtttc
    36601 caccaccact tgcatcgttt cgagattgtg aatattgaat gccggaaccg cataaccgcc
    36661 gcgctgcgcg ttgttcagca tctgctttgt cgataccacg tacattttca tatcctatcg
    36721 tttgttttcg atttcaaaat ataatgaaat tatttgtttt caaatgtcga gataacgatc
    36781 acaaaaacga caatgtgaaa attaatcaga atgacaggca aaaaaaacgg cctcccgggg
    36841 gaagccgtaa cgaagtgcgc gtgtttttat gccggatgca acgtaaacgc cttttccgtc
    36901 atacggggta gtgctgttcg tatgaggcgt atcaggcgat agtaattttg ctatcgagat
    36961 aaacgtcctg cacggcgtta atcagtttca cgccgtcagc catcgatttc ttgaacgcct
    37021 tacgtccaag aatcagcccc attccgcctg cgcgtttgtt gataaccgca gtacgcactg
    37081 catcgctgag gtcagtttca ccgcctgcag caccgccgga gtttatcaac cctgcgcggc
    37141 ccatatagca gttagctaac tgataacgca ccagatcaat cgggttttcg ctggtcaact
    37201 tgctgtacac gcgatcgtcg gtataaccgt aattaatcgc tttatagccg ccgttatttt
    37261 ccgccatttt ttgtttaacg atatctgcac ctatggtcgc cgccagatgg tttgcctgac
    37321 cggtcaggtc ggcggaaaca tggtaatcaa cgccatcttt cttaaaggcg gagttacgca
    37381 aataggccca cagcactgtc accatgccca gctcgtgcgc acgttcaaaa gccgcagaaa
    37441 tttcttcaat ctggcgacgt gactcttccg aaccaaaata gatagtcgca ccaaccgcca
    37501 ccgcgcccat gttgaaggcc tgctccacgc tggcatacag cgtttgatcg taggtattcg
    37561 ggtaacttag cgtctcgttg tgattaagtt taacgaggaa tggaatgcga tgcgcgtaac
    37621 gccgcgatac cgatgccagc acgccgtaag ttgacgccac acagttacag cccgcttcga
    37681 tcgccagttc aacaatgttt ttcgggtcaa agtagagcgg gttagcggca aatgaagctc
    37741 cggcagagtg ctcaacgccc tggtcaaccg gcagaataga aagatatcct gtgccagcca
    37801 gacgcccagt gttgtacaac gtctgcatat tacgtaacac cgctggcggg cgattattgt
    37861 caatcatcac gcggtctacg tagtcatgtc cggggagata aagctggtca gaaggaatag
    37921 tcatacaacg gtgctgtaaa aggttgtcgg cgtctttgcc aagcaactgc gcaatatctg
    37981 tcattactat gctcccgtaa attccgattg gatatcggct atggattgtc ctggcccgcc
    38041 ttttgcgggc aatcataatc ctggtcgtta caggcgagtt tttccatctt ttggatgttt
    38101 tttcagcgtt ttctgttggc tcgattcatc agaaaaaatg ttaccgcggt caagttttca
    38161 ccgcaaaggt atttaaaagg tattattaaa tggtattgtc atcgcgtacc ttacattacc
    38221 tgtcatgaag gaattaaaag atgaaaacaa cagcaaagct gtcgttcatg atgtttgttg
    38281 aatggtttat ctggggcgcg tggtttgtgc cattgtggtt gtggttaagt aaaagcggtt
    38341 ttagtgccgg agaaattggc tggtcgtatg cctgtaccgc cattgcggcg atcctgtcgc
    38401 caattctggt tggctccatc actgaccgct ttttctcggc gcagaaagtg ctggcggtat
    38461 tgatgttcgc tggtgcggtg ctgatgtatt tcgctgcgca acagaccact tttgccgggt
    38521 tcttcccgtt actgctggcc tactcactaa cctatatgcc gaccattgcg ctgactaaca
    38581 gtatcgcttt tgccaacgtg ccggatgtgg agcgtgattt cccgcgcatt cgtgtgatgg
    38641 gcactatcgg ctggattgcc tctggtctgg catgtggttt cttgccgcaa atgctggggt
    38701 atgccgatat ctcaccgacg aacatcccgc tgctgattac cgccggaagt tctgctctgc
    38761 ttggtgtgtt tgcgtttttc ctgcccgaca cgccacccaa aagcaccggc aaaatggata
    38821 ttaaagtcat gctcggcctg gatgcgctga tcctgctgcg cgataagaac ttcctcgtct
    38881 ttttcttctg ttcattcctg tttgcgatgc cactggcgtt ctattacatc tttgccaacg
    38941 gttatctgac cgaagttggc atgaaaaacg ccactggctg gatgacgctc ggccagttct
    39001 ctgaaatctt ctttatgctg gcattgccgt ttttcactaa acgctttggt atcaaaaagg
    39061 tattgttgct tggtctggtc accgctgcga tccgctatgg cttctttatt tacggtagtg
    39121 cggatgaata tttcacctac gcgttactgt tcctcggtat tttgctgcac ggcgtaagtt
    39181 acgattttta ctacgttacc gcttacatct atgtcgataa aaaagctccc gtgcatatgc
    39241 gtaccgccgc gcaggggctg atcacgctct gctgccaggg cttcggcagt ttgctcggct
    39301 atcgtcttgg cggtgtgatg atggaaaaga tgttcgctta tcaggaaccg gtaaacggac
    39361 tgactttcaa ctgggccggg atgtggactt tcggcgcggt gatgattgcc attatcgccg
    39421 tgctgttcat gatttttttc cgcgaatccg acaacgaaat tacggctatc aaggtcgatg
    39481 atcgcgatat tgcgttgaca caaggggaag ttaaatgaaa acagaacgta ttctcggtgc
    39541 tctttatggg caggcgttag gggatgcgat ggggatgccc tccgagcttt ggccgcgcag
    39601 tcgcgtcaaa gcacactttg gctggattga ccgttttctg cctgggccaa aggagaataa
    39661 cgcagcctgt tattttaacc gcgccgaatt caccgacgat acctcgatgg cgctgtgtct
    39721 ggcggatgcg ttactggaac gtgaaggcaa gatcgatccg gatctgattg ggcgtaatat
    39781 tctcggctgg gcgctgcgtt tcgacgcctt taacaaaaac gtactaggtc cgacctcgaa
    39841 gattgcgctt aacgacattc gcgacggtaa acccgttgct gaactggaaa ataacggcgt
    39901 gaccaacggc gcagcgatgc gcatctcgcc attaggttgt ttgcttccgg cgcgcgatgt
    39961 tgattccttt attgatgatg tggcgctggc ctccagcccg acccataaat ccgatctggc
    40021 ggttgcgggg gcggtagtca tcgcatgggc gatttctcgt gccattgacg gagaaagctg
    40081 gtcagcgatt gttgattcac tgccttcaat tgcgcgacat gctcaacaaa aacgcatcac
    40141 taccttcagc gcctcactgg cagcacgtct ggagattgcg ctgaaaattg tgcgcaatgc
    40201 tgacggcacc gaatccgcca gcgaacagct ttaccaggtc gttggcgcag gtaccagtac
    40261 tattgagtcc gttccgtgcg ccattgcgct ggttgaactg gcacaaaccg acccgaaccg
    40321 ttgcgccgtc ctgtgcgcta accttggcgg cgacacagac accatcggtg ctatggcgac
    40381 ggcaatttgc ggcgcgttgc atggcgttaa cgctatcgat cctgcgttaa aggcggagct
    40441 ggatgcggta aatcagcttg atttcaaccg ctatgccaca gcgctggcga aatatcgtca
    40501 acaacgggag gcggtatgag cagcgctcga ttacacacgc tgctgcctga attaaccccg
    40561 cgtcagccag ttatggtgat tggcgcggcg gtcattgatg tgatcgccga cgcttatgcc
    40621 ctcccctggc gtgggtgcga tatcgaactg aaacagcaga gcgttaacgt tggcggctgc
    40681 gcactgaata ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa tgccttgccg
    40741 ctcggtcagg gggtgtgggc ggagattatt cgcaaccgga tggcaaaaga ggacttaatc
    40801 agcctgatcg ataacgccga aggtgataac ggctggtgtc tggcgctggt tgagccggat
    40861 ggcgaacgca cttttatgtc gttcagcggc gtggaaaatc agtggaatcg ccagtggctg
    40921 gcgcgattaa ccgttgcgcc tggcagcctg ctctattttt ccggttatca actggcctcg
    40981 ccctgcggcg aattgttagt ggaatggctg gaagagctgc aagacgtgac gccatttatc
    41041 gattttggcc cacgtattgg cgatatcccg gatgcattac tggcgcggat catggcctgt
    41101 cgacctttag tgtcactcaa tcgtcaagag gctgagattg ccgccgaacg ttttgctcta
    41161 tccgcagaga taacaacact aggcaagcaa tggcaggaga aatttgccgc gccgttgatc
    41221 gttcgtctcg ataaagaggg cgcatggtat ttcagcaacg acgcttctgg ctgcattccg
    41281 gcatttccaa cgcaagttgt agacaccatt ggggcgggcg acagtcatgc cggcggcgta
    41341 cttgccgggc tggcctctgg tctgccactg gcggatgccg tattactggg caatgcagtg
    41401 gcgtcgtggg ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga ggaactactc
    41461 ctcgcacaca aaaacgtata gatcgctgcg acagtggctg atgctgtact caataggccg
    41521 ttgctgttgg tcaagcgcca cttgcttgat caccagcacc ggtattttgc tatctaactg
    41581 aatatgtgac tgaaactcgg catccggcat ccgggcgcta acgcgggaac gcgtacgttg
    41641 tgggtaaatg tgctggctgc ggaagtagtc ataaagagaa atcccgatgg catcaacatc
    41701 atgaattaaa tgtgccggaa cccacgattc ctcaatcgat accgcctctt catcgacata
    41761 gcgaatacgc ttaagcaaaa agacatcact tcccgcttcg accgccagct gctgcgcgac
    41821 ttcttcggga catttcacga cacgcttgtt aacccataac gtatcgggct ttttcccgcg
    41881 caataccacc tgctgagaaa ccccccgcgc ttctttcagc gagtattcga agatgttgtt
    41941 gatctgcgtg ccgtaaccac gcgaacgcgt caccacgccc tcttcttcca gcgactgcat
    42001 cgccttgcgc accgtaatgc gcgaaacgcc ggttaactga cttaaatcac gctcaccggg
    42061 caaaatattg ccatgctcca gcaccccgct gcgcacggca ttttttaccg tttcggcaaa
    42121 cttaatataa agcggcgtgt tatccgccgc taaaatacgc tcgttcagtt gggcaatcag
    42181 ctgggtatgc gcttgttcca tttgtctttt tcctgccggg gaatctggtg ggagtatatc
    42241 actgcttcat atttaaggcg tttctttaat cccatcaacg aatccctgca actcctccac
    42301 cgtgccatta aacacattaa aatccaccgg accattaatg ccatcgacct gtccacggtc
    42361 ggaatgctgc cagaagcgcc aggccatgcc gtcattgtcc gggcgacgtt gataatagtg
    42421 agccacccac cacggatatt cattgaaata gcccgccaga ttggtgtgat aaaaaacggc
    42481 tcctgagtaa ataattggct ttttacccgt acgtttttcg accattttta gccactgact
    42541 tacccgcttg cgtaattctt ttgccgataa ttttccccgt tcttctacgt ccagcacggc
    42601 agggaaatcg ccttgtgaga aatccaccgt ttgcagaaat aatctcgcct gaactgaagc
    42661 agataccgac ggggaaaaat aatgatacgc cccgcgcagc aggccatttt cgcggcttag
    42721 ttgccagtta cgcgaaaaat aggggtccac cagcttttcg ccttccgtcg ccttaataaa
    42781 ggcaaattgt aagcgaatac cgttatcgcg catttttgcc acccgctgcc agtcgatccg
    42841 ctcctgccag cgcgaaacat ctattccgtg aatggtgtaa ctggcaggaa tgcgaatagc
    42901 gaaagattta accgggcgat aaccgtagaa gtgaatgtag tctttaatct gaattgctgt
    42961 cgaatagaaa aagttcaccg tctgacgcgg ataaattgca acgatggaaa ttagcccaag
    43021 agcgcataat aaaacggtga atttttttcg actggtgatt ctcaactgca ttggtttcat
    43081 ccctgaatgt cagcgccaaa ggctgacaat aaccaaagca gctatagtac ggtgctcacc
    43141 accaggcgtg gaagtggtga accggaccaa taccgtgacc aacttccagc gtgtcggcct
    43201 gggctaatgc cgatgaaagc cagctttttg cctcctgtac ggtgtcagcc cagtttgtat
    43261 ggcgcgggcg cagtgcagcc aacgccgcag agagcgtaca accagtgccg tgagtgtttt
    43321 tggtcataat gcgctgtgcg gtaaaccgtt gttcaccctc gcgggtaaac agccagtccg
    43381 ggctttgctc atcatccaga tgaccacctt tcattagcac tgcgccacag cccatcgcca
    43441 acagcgatcg cccttgttcc agcatttcct gttcggtgcg cgcgtgtggc gcgtcgagca
    43501 aggcggctgc ttcgggcaag tttggcgtta ttaatgaaac ctgtggcaat aatcgactgc
    43561 gcagcgtagc aactgccgaa ggtgaaagca gcgggtcgcc gctttttgcc agcataacgg
    43621 tgtcgagtac cacgttttgg atctgataac gttgcaaccg ttctgccacc gcttcaacaa
    43681 tatcggtttc cgccaacata ccgattttag tggtgtcgat tcgcacatcg ctgaacaccg
    43741 aatcgagctg ggcggcgaca aaatcaggct caatgcgata caccgactgt acgccacggg
    43801 tattttgcgc caccagtgca gtaataactg agcaaccata agcgccaagt gccgagaagg
    43861 ttttaagatc ggcctgaatc cccgcaccac cactcggatc agtaccggca atcgtcagag
    43921 cgttaattcg tttcatgcct gcacctcctg cgtcaattgc cagagcgcat caaggaaatg
    43981 tggaacaaaa ctgcctggcc cctcgcttct ggcaactgcg cgttctccgg cttgtttcat
    44041 ccagtgacag gcagatgcga tattttccag ggtatcgcct ggtagcgcac aacaggcagc
    44101 gacaaccgcc gatagtgcac agccagttcc taccactttg gtcattaacg gatcgccacc
    44161 gtgaatgcca atgatacgat gtccatcggt aacataatcc acctcgccag tgaccatgac
    44221 gattgcgcca gtttcccgtg ccagtgtttg tgcagcgggt atcgcgttag ctgcggcatc
    44281 agtggtatcc actccccgtc caccattagc aacgccagct aatgccatga tttccgaagc
    44341 attaccacgt atggcggttg gcttatggga cagaagttcc acacaaaaac ggcggcgata
    44401 atcgagcgca ccaaccgcaa ccggatcaag cgtccagggc gtttgtgatc gtgttgcttg
    44461 ctcaacggca gcactcatcg cttgcgctcg tggctgcgtc agtgtgccaa cgttaatcag
    44521 caacgcactg gcgatagccg caaactgact ggcttcttcg gtttcgataa ccatcgctgg
    44581 cgatgcaccg agcgccagca aggtattggc agtaaaggtt tgcaccacat cgttggtcat
    44641 acagtgcaca agaggggaat gttggtgaaa aaggtgtaac gcgtgcgcag attgcgctga
    44701 actcagcagg tcgacttgca tagtttgctc ctgccataac gtgaagaagc aatgacctgg
    44761 tggtccgtga cttccctacg ctggcattat ccagatcagg tgatacgggt atttctcagc
    44821 cttcacaaag aagggcaccc cgagtcgtta ggttgcgata ataaatcgca gaggaggatg
    44881 gtaatgtcca gcgcacgcgt tgtaaacggg gaaaaacggc agttttacaa tcgcgttaat
    44941 aaattatttg atataagaat ccagcacctt cagaacgaca tccagatctt cttctcgttt
    45001 tagctcatcc ccctggtgaa cgatgtgttc cgtcagatga cctttaatca cttcccgcat
    45061 cagaccgttt accgcgccac ggatagcagc aatctgttgt aaaactgcag cgcattcatg
    45121 cggttcgtcg agcattttct tgagcgccac gacctggccc tgaatcttac tggcacgcgc
    45181 tttcagtttc tgtttatcac ggattgtatg agacatggca acacctggtt aacaagaata
    45241 tgaaaaatca tagcactatt aatctactgg ggggtagtat caggtactgg gggggagtag
    45301 aatcagattg ccgaattaat actaagaatt attatcatga ccgaatttac aacacttctt
    45361 cagcaaggaa acgcctggtt cttcatcccc agcgccatct tacttggtgc gcttcatggc
    45421 ctggaaccgg ggcactcaaa aacgatgatg gcggcgttta tcatcgccat caaaggcacc
    45481 attaaacagg cggtgatgct tggactggca gcaactattt cgcataccgc agtggtctgg
    45541 ttaattgcct ttggcgggat ggtgatcagc aaacgcttta ctgctcaatc agcagaaccg
    45601 tggctccagt tgatttccgc agtgatcatt attagcaccg cgttctggat gttctggcgt
    45661 acctggcgcg gcgaacgcaa ctggctggag aatatgcacg agcatgatca tgagcatcat
    45721 caccacgatc acgaagatca ccacgaccat ggacatcatc accatcacga acatggcgag
    45781 tatcaggatg cccatgcacg agcccatgcc aatgacatta aacgacgctt tgatggtaga
    45841 gaggtcacca actggcaaat tttgctattt ggcttaaccg gtggccttat cccctgcccg
    45901 gcagcaatta ccgtgctgtt gatttgcatt cagttgaaag ccctgacact gggcgcaaca
    45961 ctggtcgtca gtttcagcct cggcctggcg ttaacgcttg tcaccgtagg cgttggcgca
    46021 gcaatcagcg ttcagcaggt cgcaaaacgc tggagcggat ttaacactct cgctaaacgc
    46081 gctccctatt tttccagtct gttgattggc ttagttggtg tgtatatggg cgtacatggc
    46141 ttcatgggca taatgcgata actcaatcct ttaggcttgc atgttatctt cagcccagga
    46201 tgattcttaa atcagctatt tccgctgact ctctgctcgc taaggatgct tttagggcat
    46261 cctttcattt acacttttta cgaaatcatg ggatcactaa caaaatatcg cttgtcagtt
    46321 atattgtatg gcaggaaaga tatgcgactg atattacaga tccccaaagt ggagagttta
    46381 tgaccattaa aaataagatg ttgctgggtg cgcttttgct ggttaccagt gccgcctggg
    46441 ccgcaccagc caccgcgggt tcgaccaata cctcgggaat ttctaagtat gagttaagca
    46501 gtttcattgc tgactttaag catttcaaac caggtgacac cgtcccagag atgtaccgca
    46561 ccgatgagta caacattaag cagtggcagt tacgtaacct gcccgcgcct gatgccggga
    46621 cgcactggac ctatatgggt ggcgcgtacg tgttgatcag cgacaccgac ggtaaaatca
    46681 ttaaagccta cgacggtgag attttttatc atcgctaaaa aaaagccccc tcggagagag
    46741 gggggaatgc agacacctta atatttttat tattagccac ttgctcgtct tgcttgttat
    46801 tagtcgtatt tcacgttgat taatgcggtt gcctccagtg cgccagattt aactttgttt
    46861 gtatcgtaga cgtagtaact ggccgttatc ggaattgaaa ccgttgtatc tgtaagtttt
    46921 gactttgccg caatttcttt taaatttgta tatcctttac tgctgttagt gatatttaaa
    46981 agtgtgactg gcgtaccatt actttcaaga ataaatccta cgccattatc ttccgcacct
    47041 ttcaggatcg tgttgctgtt ggttaacaag tcagaacttg ataaatacac ctgcattttc
    47101 cgtgatgttt gattttgttg acctaataaa tttttacaac gcagaataat atcccctttt
    47161 ttactattta ttgttgtttt gttacctgtg gctggtaatt gtgaaacggg aatactctct
    47221 aacgtaatta ataaatcgtc cggtttgcag gttgtggtta tcgggttgta tgacaatgtc
    47281 agattaaagg aatagacacc tgcattttgt gccaatgcat ttctgtagct atttacacat
    47341 gcatcccatg acttaccacc taataaacac agtagaaatt gtactgcggc actcccgcgt
    47401 gtcgatgatg aagcactgga taatgtcgca actccattaa tcatcactga accaccgccc
    47461 cccgcagaaa cattcacttt tgcattactg gccgcaggtg tcaccgtcac cgaataacca
    47521 ctggtgaaac tggtctgatt atttgacaac gtaatgttgt ttttattggt ccattcaaaa
    47581 tgtaaattta atttaatggt atcgttataa agtccaacaa taatatccga cgggctcata
    47641 ggtgtgatag tatttgtact cacgcatgac gaatcattaa aattatgctc atatgtcgcc
    47701 atttgtgttt gagtaagtgc aaaatccccc tgaacaacca aattgtgctg aacagataac
    47761 tcactataac atgccgcata gctcacaggc ataaccatca tcattactat aaatagcatt
    47821 atgcgaattt ccatagaacc acctgactat tactggcaaa tataatttct actttcatca
    47881 atctctttac cgaaggtgat tgtgcatgaa agcccttgtt gcttatcaat tgccacatta
    47941 accgatggcg gtacttcatt ggtgcgaata aataactggc ttccctggcc gacaacacca
    48001 atattatgac catggatatc attgacttca taaccaaacg ttaatggttg cccatccgct
    48061 cttaacgctt ttataaacca tggcttgcgc tgatcggtat caaaattaac cagtacaacc
    48121 gcgccgcgat aaggggcggc aattttccgg ttgccacgta attctgcttc gctatcgctt
    48181 tgtgacacat ccaacatcag gtaattttcc cgataaggtg tcattccgtc gtataccacc
    48241 actccattac ggtttgttgt gcgatatttt tgcccattga cataagcatc tttaattcct
    48301 ggcgcattca tcacagcaaa cgtttcagaa agacggtttg ccagattaac gccacccgac
    48361 caggcgacaa tgccccctga aacactggct ccagcctgtc gataagcact cgactgacta
    48421 taactgccat tcactgtcgc aaccggcgcg ttccaggtta aattcgcccc agctgtcgtt
    48481 tcatttccct gatactgata actcaggttg accccatagt taaactgatc ccggcttcca
    48541 acggttcctg ataatcccgt attatttgag gcaaccccct gatcatcaaa cgtcgttgag
    48601 ttagacatat atatttgccg acgaggcgtc gtaacgtcat caccccaatc aaagggaatc
    48661 gatataaaaa tattaaaacg tttctcttca tgatgattct cgtcataagc atggcttgcc
    48721 gcgagggtat agcttatcct tcgcaagttg ttggaataac tcaactgata atccttactg
    48781 ctgccgctac gcccccagta atctcgccat aacgtactta aggacacaga accccagcct
    48841 tctggcaatg actgactcat attggcagag aagctatttt tacggccaaa atcgttctgg
    48901 taataatcgg caatgtcata gatatcgttt tcatcatggc gataattatc tttattgttt
    48961 gcccatacgt gatcattaaa tgtccgataa tcacgcgacg aataacgcca ggccgccaga
    49021 ccaaaacgtg tcgacgtttg gctcacaaat ttgttgtagg caatttgata actttgcccg
    49081 tcaaacacat cgccgttgtc ttgtttacta tgcgacttcg tggcatcgac ggaaatggca
    49141 ccaatgcgtg tattccagcc agtccccaaa gtgaacgcgt aataattatt cgcgaccatc
    49201 gagccaccat acagcgttaa taagttatta aaaccacgcc tggacaaaat cactttgttt
    49261 gctcgcccct tcaatatggc tacgacccgc cgcaaaatca tatttcgaca cgccgggttg
    49321 cagcatattt ggtaccgctg cataaggcac cagataggtg gttaccgaac cgtccgcctc
    49381 tttcacgctg acatcaagat ccgctccacc accggccaac tgcaaatctg taatcgcaaa
    49441 cgggccagga ggaacctctt tctgataaac cacaaaacca ttctgttcaa tagttaccag
    49501 cgcgttactc tgagcaatcc cctgcacccg tggcgtaaaa ttttgtttcg agttaggcaa
    49561 catctgcata tcacgaaaca accgcacacc gctgaagcga acagaatcaa aaatatcgct
    49621 tgatgtgtac atatcaccca cacgaagcgt gccgagaagt tgggcaaatc cacgttccag
    49681 atacagggta ttgcttttcc acacccctgg attgttattt gttttactga aactggcatc
    49741 agaatgcagt tgccacccca ataaatttaa cccgctgtta aaacgtacat atgtactctt
    49801 gctattaccc gacgctttat agtcgctgta atactgactc acataataag aggtataaaa
    49861 cgcattaata ccccgctccc agttttcagg tggtacatag ccactttcca gttcttccac
    49921 ctaggcctgc gggacactga aatcgagacg aaaaactccg atatcccagg tatagctccc
    49981 gccctgaata agttgcttaa atgttaaaca ttgcttaccg ctggcaaagc tatcggtatt
    50041 aatgcctaac cgcttgatca tttcccttga taaacatgtt tcttgcgggt tgtctttaac
    50101 aatgatctca tatttcccac gccattgctt attgacataa atatcgatgt catactgccc
    50161 cggcaagggc tgattatctt caagacgaat atttgcaacc tgctggtctt tcattccacc
    50221 tatcatgaaa tgggtatcaa aggtttcttc agctgcataa gcttcaatgc cgatcaataa
    50281 cgctacgatt gctgatgcaa gtggggtcat tctcaacata ggcagctcct gaaattaaat
    50341 tttgtcacta atataattgc catggtcatc gataatggtc agatgccagt tatttgcatt
    50401 attacttttg acattaacac tctaactttc taagggggca atcataatag tttcataatt
    50461 gactttgaca ttattagctt tgacatccga aatcgtcacc caattagctg aggcattttt
    50521 tatcaccaat ccattgccac tgcgatttac cagtaacttt ttaaaggtcg ctttatttac
    50581 cggagcaata cccgctggcc ggtaaaacaa cttaattctg ttttgcatcg caaacttcag
    50641 tgcattctta ccttcttgtt ctggactatt tggtggaata tccagaacat tcagataaaa
    50701 aatactctct ttattagtgg gtagtttatt cggcataatt ttaattttta cttgctgccc
    50761 ggaatttgcc cctatttttg ccactggtgg cgttaacatg aaaggaacct gaattttttc
    50821 tggtggtaat gacgtatcgc catcatcaat ccacgcctgc atcagcgaag aacggtttcc
    50881 ctggttcatc aactgcacca tcacttcttt gtgctctgcc gggtaaataa tacgcgtccc
    50941 gtagataaca ataccggcat gtgcaggaag gaccatagaa gaaatgagta aagctaataa
    51001 tcctttcatc ttaatgcctc ttaaattaaa aggccgccat ggagatagcg gccatgttaa
    51061 ttacctgaag aatcttgcaa ttttatttat taatcctgcg ccacacgcat aatcagggaa
    51121 gataacacat cacctgaatt cactgagtta tctgtcagtt tcaggtattt tgctttaaac
    51181 ggaatggcat attgaccgtc cgctgtttta taactctcga tgttgaaagg tgcagaaaca
    51241 tccaggacat cgctgttatc acctaaatag agataataac ccacggtatc gttgctactg
    51301 gctatcgcct tatcgtttcc ggttatttta tcgttggtaa tcaacgagag ttttacacca
    51361 gcaacttctt tcggacagct aatttttaac atgctgctgg ttacaccgac tgcaccatca
    51421 taggtcgagt atttagcttt accttcaaag accgtagttg gtacagttgg catattaata
    51481 cttactgaat cgccacctgc ttcgaattta cacggagatt ctgcaatatt ccctttaatg
    51541 gttaatgttc cggtatcaac gtctgcggca aataccccag cgctcataaa aaaagaagaa
    51601 aaaacagcgg cagcaataat tgaacgtttc ataatatgct ccttaataaa atctattttt
    51661 ggttgttaag agtctgtatc tcttggaaac aaatttctca caccaaaaaa
//
LOCUS       DAZGJT010000024        51417 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZGJT010000024 DAZGJT010000000
VERSION     DAZGJT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51417
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.024"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..864
                     /gene="uidC"
                     /locus_tag="ACHKFK_001529"
     CDS             <1..864
                     /gene="uidC"
                     /locus_tag="ACHKFK_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO9051897.1"
                     /translation="SLPYRIPLMAGSSQRTLPTVSEGALGYWALTPNIDLWGMWRSRV
                     FLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDTSRYSLGASVQKDVSNQIQ
                     SILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETALVSGQLTWIAPWGSVFGS
                     GGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVNYRLTPQFTLTFAPIVTRG
                     YESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKGREKRDGSALGDRLNYWDV
                     KMSIQYDFMLK"
     gene            complement(909..2417)
                     /gene="ydgA"
                     /locus_tag="ACHKFK_001530"
     CDS             complement(909..2417)
                     /gene="ydgA"
                     /locus_tag="ACHKFK_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9051898.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKVNPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTVNSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(2518..3693)
                     /gene="manA"
                     /locus_tag="ACHKFK_001531"
     CDS             complement(2518..3693)
                     /gene="manA"
                     /locus_tag="ACHKFK_001531"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO9051899.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVHDAAGDIVSLRDVIESDKATLLGDAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPDAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLCKGSQQLQLKPGESAFIAANESPVTVRGHGRLARVYNKL"
     gene            3892..5538
                     /gene="fumB"
                     /locus_tag="ACHKFK_001532"
     CDS             3892..5538
                     /gene="fumB"
                     /locus_tag="ACHKFK_001532"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9051900.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            5681..7084
                     /gene="fumC"
                     /locus_tag="ACHKFK_001533"
     CDS             5681..7084
                     /gene="fumC"
                     /locus_tag="ACHKFK_001533"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO9051901.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DRWVRPEQMVGSMKAGG"
     gene            complement(7081..8010)
                     /gene="tus"
                     /locus_tag="ACHKFK_001534"
     CDS             complement(7081..8010)
                     /gene="tus"
                     /locus_tag="ACHKFK_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO9051902.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNFSQAALVSHIQHINKLKTTFEHIVTVESELPSAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(8086..9387)
                     /gene="rstB"
                     /locus_tag="ACHKFK_001535"
     CDS             complement(8086..9387)
                     /gene="rstB"
                     /locus_tag="ACHKFK_001535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFO9051903.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGVAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(9391..10110)
                     /gene="rstA"
                     /locus_tag="ACHKFK_001536"
     CDS             complement(9391..10110)
                     /gene="rstA"
                     /locus_tag="ACHKFK_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO9051904.1"
                     /translation="MNTIVFVEDDAEVGALIAAYLAKHDMQVTVEPRGDRAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATVTKGIQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            10239..10574
                     /gene="ydgC"
                     /locus_tag="ACHKFK_001537"
     CDS             10239..10574
                     /gene="ydgC"
                     /locus_tag="ACHKFK_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO9051905.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLASLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(10571..11293)
                     /gene="folM"
                     /locus_tag="ACHKFK_001538"
     CDS             complement(10571..11293)
                     /gene="folM"
                     /locus_tag="ACHKFK_001538"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO9051906.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(11330..12712)
                     /gene="ydgI"
                     /locus_tag="ACHKFK_001539"
     CDS             complement(11330..12712)
                     /gene="ydgI"
                     /locus_tag="ACHKFK_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9051907.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAIMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVERPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(12898..13842)
                     /gene="ydgH"
                     /locus_tag="ACHKFK_001540"
     CDS             complement(12898..13842)
                     /gene="ydgH"
                     /locus_tag="ACHKFK_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO9051908.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            14366..15898
                     /gene="pntA"
                     /locus_tag="ACHKFK_001541"
     CDS             14366..15898
                     /gene="pntA"
                     /locus_tag="ACHKFK_001541"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO9051909.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWMASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            15909..17297
                     /gene="pntB"
                     /locus_tag="ACHKFK_001542"
     CDS             15909..17297
                     /gene="pntB"
                     /locus_tag="ACHKFK_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO9051910.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALIVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(17322..18356)
                     /gene="tqsA"
                     /locus_tag="ACHKFK_001543"
     CDS             complement(17322..18356)
                     /gene="tqsA"
                     /locus_tag="ACHKFK_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFO9051911.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQAIEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGQVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTTGGQSI
                     AVLLSDLNKE"
     gene            18768..19133
                     /gene="mdtJ"
                     /locus_tag="ACHKFK_001544"
     CDS             18768..19133
                     /gene="mdtJ"
                     /locus_tag="ACHKFK_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO9051912.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            19120..19449
                     /gene="mdtI"
                     /locus_tag="ACHKFK_001545"
     CDS             19120..19449
                     /gene="mdtI"
                     /locus_tag="ACHKFK_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO9051913.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(19488..20309)
                     /gene="ydgD"
                     /locus_tag="ACHKFK_001546"
     CDS             complement(19488..20309)
                     /gene="ydgD"
                     /locus_tag="ACHKFK_001546"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO9051914.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKAAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(20409..20492)
                     /locus_tag="ACHKFK_001547"
     CDS             complement(20409..20492)
                     /locus_tag="ACHKFK_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051915.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(20585..20893)
                     /gene="asr"
                     /locus_tag="ACHKFK_001548"
     CDS             complement(20585..20893)
                     /gene="asr"
                     /locus_tag="ACHKFK_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO9051916.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNAKAEQKAPEQKAQAAKKHAKKHSHQQPAKPAAQPAA
                     "
     gene            21164..21268
                     /locus_tag="ACHKFK_001549"
     CDS             21164..21268
                     /locus_tag="ACHKFK_001549"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO9051917.1"
                     /translation="MNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            complement(21318..22571)
                     /gene="ynfM"
                     /locus_tag="ACHKFK_001550"
     CDS             complement(21318..22571)
                     /gene="ynfM"
                     /locus_tag="ACHKFK_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9051918.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHDILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHHRLHA"
     gene            22678..23571
                     /gene="ynfL"
                     /locus_tag="ACHKFK_001551"
     CDS             22678..23571
                     /gene="ynfL"
                     /locus_tag="ACHKFK_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9051919.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTEAGKQFLVDSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPSVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYNL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVTIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            23706..24926
                     /gene="mlc"
                     /locus_tag="ACHKFK_001552"
                     /gene_synonym="dgsA"
     CDS             23706..24926
                     /gene="mlc"
                     /locus_tag="ACHKFK_001552"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO9051920.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            25051..25746
                     /gene="bioD"
                     /locus_tag="ACHKFK_001553"
     CDS             25051..25746
                     /gene="bioD"
                     /locus_tag="ACHKFK_001553"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9051921.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(25699..26991)
                     /gene="clcB"
                     /locus_tag="ACHKFK_001554"
     CDS             complement(25699..26991)
                     /gene="clcB"
                     /locus_tag="ACHKFK_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO9051922.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATIVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLISNLINHSDAL
                     LYSVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLFAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAKHS"
     gene            complement(27152..27766)
                     /gene="dmsD"
                     /locus_tag="ACHKFK_001555"
     CDS             complement(27152..27766)
                     /gene="dmsD"
                     /locus_tag="ACHKFK_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO9051923.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTRDGWET
                     QWPLPEASLAPLVTAFQTQSEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(27809..28663)
                     /gene="ynfH"
                     /locus_tag="ACHKFK_001556"
     CDS             complement(27809..28663)
                     /gene="ynfH"
                     /locus_tag="ACHKFK_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO9051924.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHII
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAATILRAARVTFNTTPFAIISILALIACAGLIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVMGLILILGGEMIGRVLFYCLHM
                     TVGMAIAG"
     gene            complement(28665..29282)
                     /gene="ynfG"
                     /locus_tag="ACHKFK_001557"
     CDS             complement(28665..29282)
                     /gene="ynfG"
                     /locus_tag="ACHKFK_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO9051925.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(29293..31719)
                     /gene="ynfE"
                     /locus_tag="ACHKFK_001558"
     CDS             complement(29293..31719)
                     /gene="ynfE"
                     /locus_tag="ACHKFK_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO9051926.1"
                     /translation="MSKNEQMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAEAAV
                     QQANEKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVKQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDKTAKTPQWASQITGIPVDRIIKLAREIGTAKPTYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFR
                     AFRVDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLR
                     KTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNN
                     GEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPS
                     HSNLVQIEKV"
     gene            complement(31918..32223)
                     /gene="ynfD"
                     /locus_tag="ACHKFK_001559"
     CDS             complement(31918..32223)
                     /gene="ynfD"
                     /locus_tag="ACHKFK_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO9051927.1"
                     /translation="MKLSTCCAALLFALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDNAPAEPQ"
     gene            32331..33041
                     /gene="ynfC"
                     /locus_tag="ACHKFK_001560"
     CDS             32331..33041
                     /gene="ynfC"
                     /locus_tag="ACHKFK_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO9051928.1"
                     /translation="MKYKLLPCLLAILLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDNHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            complement(33044..33604)
                     /gene="speG"
                     /locus_tag="ACHKFK_001561"
     CDS             complement(33044..33604)
                     /gene="speG"
                     /locus_tag="ACHKFK_001561"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO9051929.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(33639..33980)
                     /gene="ynfB"
                     /locus_tag="ACHKFK_001562"
     CDS             complement(33639..33980)
                     /gene="ynfB"
                     /locus_tag="ACHKFK_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO9051930.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QRAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVIIP"
     gene            34115..34441
                     /gene="ynfA"
                     /locus_tag="ACHKFK_001563"
     CDS             34115..34441
                     /gene="ynfA"
                     /locus_tag="ACHKFK_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO9051931.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALIWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            34647..35861
                     /gene="rspA"
                     /locus_tag="ACHKFK_001564"
     CDS             34647..35861
                     /gene="rspA"
                     /locus_tag="ACHKFK_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO9051932.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFDEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGEKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            35873..36892
                     /gene="rspB"
                     /locus_tag="ACHKFK_001565"
     CDS             35873..36892
                     /gene="rspB"
                     /locus_tag="ACHKFK_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO9051933.1"
                     /translation="MKSILIEKPNQLSIIERKIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGDGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GESFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(37080..38375)
                     /locus_tag="ACHKFK_001566"
     CDS             complement(37080..38375)
                     /locus_tag="ACHKFK_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO9051934.1"
                     /translation="MREAKMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTTKNRR
                     VAGELRASVCYAIKTGVFDYAKQFPSSHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMTLTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSARVKNYG
                     DHFTVDSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVALLNTQLSEFAPTMPQNEVVQN"
     gene            complement(38395..38646)
                     /locus_tag="ACHKFK_001567"
     CDS             complement(38395..38646)
                     /locus_tag="ACHKFK_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9051935.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            complement(38716..41196)
                     /locus_tag="ACHKFK_001568"
     CDS             complement(38716..41196)
                     /locus_tag="ACHKFK_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO9051936.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NATFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSAVDLARA
                     RVNRQHTENSAENISPVTAVIRREYKQTWKTLDDELAYALWPGDIDAGNIDGSIHRWA
                     KNEVINNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQIATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLDKALAAGATEITKEEYFVRKNSTYSGSQQNTGASDTTAQ
                     PGPVKVTADEVNNIMQAANISQPDADELLAVSRGEFVAGISDPNDPKWVKGMETRDSV
                     NQNQQETEQNGQKAEQNSPNALQNEPETKQPEPVVQQEPEKICTACGQTGGGNCPDCG
                     AVMGDATYQETFNEENQVEVQENDPEEMEGAEHPHKENAGSAQDHASDNETGETADPL
                     IAVNGHHVITSTSRVWYHLMIDLETMGTNTNAPIVVIGAVFFDPQTGEIGPVFYIVIS
                     LTDAMNTGAVPDGGTIEWWLKQSSEARAAILTDQVKLKDALSQFREFINEYSDEKFVQ
                     VWGNDATFDNAILRTSYERLDIPCPWRYHNDRDVRTIVELGKTIDFDARTVIPFEGVR
                     HNALDDARHQAKYVTATIQKLIPSQADF"
     gene            complement(41277..41480)
                     /locus_tag="ACHKFK_001569"
     CDS             complement(41277..41480)
                     /locus_tag="ACHKFK_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO9051937.1"
                     /translation="MNTTIALTLTVFLNTGEPVDMVIDIYGSMKECMAAAAEQKIPGN
                     CYPVDKVILMDNNEIPAGLKTAP"
     gene            complement(41477..41665)
                     /gene="dicB"
                     /locus_tag="ACHKFK_001570"
     CDS             complement(41477..41665)
                     /gene="dicB"
                     /locus_tag="ACHKFK_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO9051938.1"
                     /translation="METLLPNVNTSEGCFEIGVRISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPMQKGYWQ"
     gene            42232..42478
                     /locus_tag="ACHKFK_001571"
                     /pseudo
     CDS             42232..42478
                     /locus_tag="ACHKFK_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(42456..42863)
                     /locus_tag="ACHKFK_001572"
     CDS             complement(42456..42863)
                     /locus_tag="ACHKFK_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001359738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051939.1"
                     /translation="MEFKDLPKEIQIIAATTLGDSLVKIDPAYTKKETIDNMVRNVRN
                     AFSGLYGSDNQKQKNDIDERVISVCLNGHVISAIRTETATVFDYLCMIQSLADVLLKS
                     KDLENDANLQGRTIAHPYAHTLGSVDIKDPTNL"
     gene            complement(42886..43104)
                     /gene="ydfC"
                     /locus_tag="ACHKFK_001573"
     CDS             complement(42886..43104)
                     /gene="ydfC"
                     /locus_tag="ACHKFK_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO9051940.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            complement(43134..43262)
                     /gene="ydfB"
                     /locus_tag="ACHKFK_001574"
     CDS             complement(43134..43262)
                     /gene="ydfB"
                     /locus_tag="ACHKFK_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO9051941.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSPAKSNG"
     gene            complement(43264..43419)
                     /gene="ydfA"
                     /locus_tag="ACHKFK_001575"
     CDS             complement(43264..43419)
                     /gene="ydfA"
                     /locus_tag="ACHKFK_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO9051942.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTESGARRW
                     LERDSGG"
     gene            complement(43586..43993)
                     /gene="dicA"
                     /locus_tag="ACHKFK_001576"
     CDS             complement(43586..43993)
                     /gene="dicA"
                     /locus_tag="ACHKFK_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9051943.1"
                     /translation="MDTKNLTIGERIRYRRKNLKYTQRSLAKALKISHVSVSQWERDD
                     SEPTGKNLFALSKVLQCSPTWILFGDEDKQPSPPIEEPVALSPKEHELLELFNALPES
                     EQDAQLTEMRARVKNFNKLFEELLKARQRTNKR"
     gene            44077..44307
                     /gene="dicC"
                     /locus_tag="ACHKFK_001577"
     CDS             44077..44307
                     /gene="dicC"
                     /locus_tag="ACHKFK_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000921592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicB transcriptional regulator DicC"
                     /protein_id="HFO9051944.1"
                     /translation="MLKTDALLYFGSKTKLAQAAGIRLASLYSWKGELVPEGRAMRLQ
                     EASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            44291..44812
                     /locus_tag="ACHKFK_001578"
     CDS             44291..44812
                     /locus_tag="ACHKFK_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO9051945.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRKKFTALIPAIMEAIRINDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVDRETDELIEKAIAFKLAYRQQY
                     QQKAGWNYESSFC"
     gene            44793..45758
                     /locus_tag="ACHKFK_001579"
     CDS             44793..45758
                     /locus_tag="ACHKFK_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051946.1"
                     /translation="MSLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTNSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLMRCEKLNKSKRDMTNFYTINY
                     GSELLDGGKVSESIGSKCAAPSGQNDTMEEVKMKRSIGSKRLNVIGSKWPDDLTENTT
                     EITTENKNTSRPEASQPDPQTVEQDFLTRHPDAVVFSAKKRQWGNQEDLACAQWIWGR
                     IVSLYEQAASDDGEISRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRAQRDPFWV
                     KNIMSPSKLRGKWDELVIRLGRSSVQRCVNHISEPDTEIPPGFRG"
     gene            45764..46510
                     /locus_tag="ACHKFK_001580"
     CDS             45764..46510
                     /locus_tag="ACHKFK_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9051947.1"
                     /translation="MKNIAAGGVLERIRKLTPQHVIAPYRTVDEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNHLLAKGRSVIVITVADVMLALHNSYDNKNSGEKFLQG
                     LCDVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASIRSVGMLTNLNHVAMSTLLGERV
                     MDRMVMNGGRWVNFNWESWRSNVRHLRVVK"
     gene            46525..46941
                     /locus_tag="ACHKFK_001581"
     CDS             46525..46941
                     /locus_tag="ACHKFK_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001409082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9051948.1"
                     /translation="MVKVFTPEQREEVKARIVELVRRDGRKTRKQLENETGATRHLIE
                     VLAKELVDSGVVYGSGHGIFPSEQVRKDWIKAHKEMSKGAAKKKSDPGLIYSLPDGEI
                     RHYDRRQNIICLECRKSKVMQRVLAFYQGNFQEVMA"
     gene            46938..47243
                     /locus_tag="ACHKFK_001582"
     CDS             46938..47243
                     /locus_tag="ACHKFK_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFO9051949.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKAEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            47230..47514
                     /locus_tag="ACHKFK_001583"
                     /pseudo
     CDS             47230..47514
                     /locus_tag="ACHKFK_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
     gene            47782..48198
                     /locus_tag="ACHKFK_001584"
     CDS             47782..48198
                     /locus_tag="ACHKFK_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051950.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIALASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMPVKRPAD"
     gene            48849..49004
                     /gene="hokD"
                     /locus_tag="ACHKFK_001585"
     CDS             48849..49004
                     /gene="hokD"
                     /locus_tag="ACHKFK_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO9051951.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            49220..49399
                     /locus_tag="ACHKFK_001586"
     CDS             49220..49399
                     /locus_tag="ACHKFK_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051952.1"
                     /translation="MSCPLKKHTRLSIPPRDKSVVAVPRPVIDENCAHREQVKNAFDF
                     GFSRYEKAMEELSKV"
     gene            49418..49903
                     /locus_tag="ACHKFK_001587"
     CDS             49418..49903
                     /locus_tag="ACHKFK_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFO9051953.1"
                     /translation="MAEIVEGVHYLTVDDLVEINHSLIELQTPDEPVGVLSPDNLSSS
                     QARPSMVRWYEQTNDMFVLASVLIESLIQNHPFANANKRTAMMAGYVFLLLNGYELTA
                     PGDEIVEMAEGLACKTYTREDLENWLCYWSRAYDSQELCKTSATIVLYETIKLKIEQQ
                     N"
     gene            50104..50364
                     /locus_tag="ACHKFK_001588"
     CDS             50104..50364
                     /locus_tag="ACHKFK_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051954.1"
                     /translation="MKIKELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGAA
                     LIHLVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFEDE"
     gene            50431..50709
                     /locus_tag="ACHKFK_001589"
     CDS             50431..50709
                     /locus_tag="ACHKFK_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051955.1"
                     /translation="MAQNSRLHNSDNSAAFASRHGRRSHAFKYDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRLWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            50711..>51417
                     /locus_tag="ACHKFK_001590"
     CDS             50711..>51417
                     /locus_tag="ACHKFK_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9051956.1"
                     /translation="MRVLLRPVPVPELGLVVLKPGRESMQVFHNPRVLVEPEPKSMRG
                     LPSGVVPAVRQPLAEYKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGIGAMVLCWHCDNQLRDQTSESLGQLAHQNLSAWMIDVIRHAISGTQERE
                     LSLAELSWWAVCNQVADALPESVLRRSLGLPVKKIRSVYSESDIVPGEQTATSILKQR
                     TKNIALPPHVHQHQQQNP"
BASE COUNT        13087 a        12132 c        13060 g        13138 t
ORIGIN      
        1 agcttacctt atcgtattcc gttaatggct ggcagttcgc aacgtacatt accgacagtt
       61 tctgaaggag cattaggtta ttgggcttta acgccaaata ttgacctgtg gggaatgtgg
      121 cgttcacgag tatttttatg gaccgattca acaaccggta ttcgtgatga aggggtgtat
      181 aacagccaga cgggaaaata cgataaacat cgcgcacgtt cttttttagc cgccagttgg
      241 catgatgata ccagtcgcta ttctctgggg gcatcggtac agaaagatgt ttccaatcag
      301 atacaaagta ttctcgagaa aagcataccg ctcgacccga attatacgtt gaaaggggag
      361 ttgctcggct tttacgcgca actcgaaggt ttaagtcgta ataccagcca gcccaatgaa
      421 acggcgttgg ttagtggaca attgacctgg attgcgccgt ggggaagcgt atttggcagt
      481 ggtggttatt tgcgccatgc aatgaatggt gccgtggtgg acaccgacat tggctatccc
      541 ttttcattaa gtcttgatcg taaccgtgaa ggaatgcagt cctggcaatt gggcgttaac
      601 tatcgtttaa ctccgcaatt tacgctgaca tttgcaccga ttgtgactcg cggctatgaa
      661 tccagtaaac gagatgtgcg gattgaaggc gcgggtatct taggtggtat gaactatcgg
      721 gtcagcgaag ggccgttaca agggatgaat ttctttcttg ctgccgataa agggcgggaa
      781 aagcgcgatg gcagtgcgct gggcgatcgc ctgaattact gggatgtgaa aatgagtatt
      841 cagtatgact ttatgctgaa gtaagaaaaa acgccggaga gaaaaatctc cggcgttttg
      901 gctgtgaatt actgctgcgg aatagcgggt acagccggaa cgtttaatgc cggcatcgca
      961 aacataccca cgaaatcttc cagtggcatt ttctgcccgt ttaacgttat ctgaccgtta
     1021 gcatattgca ggctggtggt gatggtattg tcctgcaagg tggtcagacg gaacatctgc
     1081 cccattgctg atgcaccttc aacttgctgt ttcgccagtt ttttcgcttg atcttcctga
     1141 taaccttcca gcttcgctac ctgagtcatt aactcagttg ccatatccac cggaatggtc
     1201 agtttcgcat ccagagattt aaccgaccgg tccacttcct gcgccagcgt ttgcggcgct
     1261 tctttagtcg ttgccggatc tttcaggaac agcgacagat tcagcgcact ttcaccctga
     1321 ctgtttttcc agcttagcgg cgcgatagta atcaccggat cgcctttcag catcagcggc
     1381 agagcgctaa agaaggcttc cgtcactttc tcctgataaa gttcgggatt gttggcaatt
     1441 tctggctgtg ccagcagcgc ctgagtttgc gcgttatatt gctggctaaa ctgatgccag
     1501 gcttcgccat caatttggcc gacttttaaa gtcagcttgc cactgcccag atcctgattc
     1561 tgtaccttca ggctgtttag cgagtaatcc agttggctat tgaccgtttt accgtcattg
     1621 accagatccg atttaccgct gatctccatg ccttccagca gtgccagttc tttgccctcc
     1681 actgaaatgg tcattttttc cagcgacagt ttttggtttc ctacgcgctc accaaaactt
     1741 gccagcgtgc tggaaccgtc ggttttcaga ttattaaagg ttaactgcac tttctggttg
     1801 tattcgttaa ccgcgtctat ccgaccactt tgcgcctccc cggaaaggga gatagctttg
     1861 ccgtctctgt ctgcatttaa ctggaactcg ccgccgctaa atgcgacttt ttcatccttt
     1921 tgctcgtaat tcagtggctt gagcgaaatg tcggaactgg aatctccgct gtaaccaatg
     1981 cgggagttaa tctcaaaagg cgtttcacct tttgccatat caaacagtgg tttgcttact
     2041 tcgttattaa ccagcgtggt ttgaattgat gccatcgacg ggatcaggtt cagtttttta
     2101 agctgggcaa gcgggaaggg accatgatca accgattcgt tgaagatgac gctctgaccg
     2161 cttttaatcc acggatttac ttttccggca atgggtttca ccaacagttg caactggctg
     2221 ctgaatacgc cgcgatgata gttttgataa ctgacttcca ggttggattc aggagctgtc
     2281 agtttgagtt gcgcgttcgc ctgcgcgacc atgtcttcga gatgggtttc aatcttcttg
     2341 cctgtatacc atgcgccgcc tgtccagact acgcctagcg caacaatgac gcctaccgct
     2401 accagcgatt tattcataat gattatccat aaaatgaaat caggcggact ggccgcctga
     2461 aggtgttata agcctttaat aagcttagca agagatgtta attttttcag taagctctta
     2521 cagcttgttg taaacacgcg ctaaacggcc gtggcctctg acagtcaccg gtgattcgtt
     2581 ggcggcaata aacgctgatt cacccggttt aagctgtaac tgctgagaac ctttacacag
     2641 cgttgcatcg ccttcgacgc agaacaaaat ggcggcactc tgctggctga tggtggtttc
     2701 tttatcacta aggtcatgca acgagaaggc aaaatcatcc acaggaatcg ggaagtccag
     2761 ttctgcacct tgtttcaccg gctgggtcaa caactggtta gccggtttgg cttcgaactt
     2821 cacgttggca accagttccg gaatatcaat atatttaggc gtcagacctg cacgcagcac
     2881 gttatcggag ttcgccatca cttccagcgc cacgccttgc aggtaagcgt gcggtgtttc
     2941 agcgaacagg aacatcgctt cgccaggatt caatttcacc acattcagca acagcgggga
     3001 gaacagaccg ctgtcttccg ggtaaaattc agaaattaaa cgaatcgttt gccatggttc
     3061 accctgctgg ctgtcgaggg ccgattttaa aatcgccagc gcgcgggatt tttcttcacc
     3121 ctgcatattc aacaggctgg cgaacagttc gcttaaacgt tcggcatcag gctgttgtaa
     3181 aaagtgagca atcgccggat gtgcacctgc gactggctgg agcagggaga caatctcgga
     3241 aaactcacga aacgcgttca tcgcgaggaa aggggtcagg gcgaaaacca gctcaggctt
     3301 gtgattagga tctttatagt tacgctcagc ggcatccatc ggaatacctg cagcattttc
     3361 tttggcaaaa ccgatttcag aattgcgttt gtttggatga acctgaatgg agagcggctg
     3421 ggctgcgcat aatactttga acaggaaagg caactcgcca aagcgtttgg caacggcatc
     3481 tcccaaaaga gtcgctttat cactctcaat cacatcacgc agtgaaacga tatctcctgc
     3541 ggcatcgtgc acacgtgaac tgctctttgg atgcgcgccc atccacaact cggccatcgg
     3601 ctggctggac ggattttcca taccataaag ttcagtcaac gccgttttgc tgccccaggc
     3661 atagttttgc actgagttaa tgagtttttg catgatcaat ccctgtttta atgtggaaat
     3721 taatcccgct attaaagcaa gaatcctacg ggaagtaacc tggagccgca aaaagtcgta
     3781 ctagtctcag tttttgttaa aaaagtgtgt aggatattgt tactcgcttt taacagggca
     3841 acggaacacc cgcccagagc ataaccaaac caggcagtaa gtgagagaac aatgtcaaac
     3901 aaaccctttc attatcaggc tccttttcca ctcaaaaaag atgatactga gtattacctg
     3961 ctaaccagcg aacacgttag cgtatctgaa tttgaagggc aggagatttt gaaagtcgca
     4021 cccgaagcgt taactctgtt ggcgcgtcag gcgtttcatg atgcgtcatt tatgctgcgt
     4081 ccggctcacc aacaacaggt ggccgacatt ctgcgtgacc cggaggccag cgaaaatgat
     4141 aaatatgtgg cgctgcaatt cctgcgtaac tccgacatcg cggcaaaagg cgttctgcca
     4201 acctgtcagg ataccggcac cgcgattatt gttggtaaaa aagggcagcg tgtatggacc
     4261 ggtggcggtg atgaagcagc gctggcgcgc ggtgtttata acacttatat cgaggataat
     4321 ctgcgctact cgcaaaacgc gccgctggat atgtataaag aggtgaatac cggcaccaac
     4381 ttgccagcgc agatcgatct ttatgccgtt gatggcgacg agtacaaatt cctctgtatc
     4441 gccaaaggtg gcggttcggc aaacaagacg tatctctatc aggaaaccaa agcgttactg
     4501 acgccgggaa aactgaaaaa ttacctggtt gagaagatgc gcacgctggg tacggcggcc
     4561 tgtcctccat atcatattgc gttcgttatt ggtggaactt ctgcagaaac gaatcttaaa
     4621 acggtgaaac tcgcttccgc taaatactat gatgaactgc caacggaagg gaatgagcac
     4681 ggtcaggcgt tccgcgatgt ggaactggaa aaagaattgc tgatcgaagc gcaaaatctt
     4741 ggtctgggtg cgcagtttgg tggtaaatac ttcgctcatg acatccgcgt gattcgcctg
     4801 ccacgtcacg gcgcatcctg cccggtcggt atgggcgtct cctgttctgc tgaccgtaat
     4861 atcaaagcga agatcaaccg tcaggggatt tggatcgaaa aactggaaca taatccaggc
     4921 aaatatatcc cggaagagct gcgcaaagca ggagaaggcg aagccgtgcg cgttgacctt
     4981 aaccgtccga tgaaagagat cctcgcacag ttgtcgcagt atcccgtttc tacacgctta
     5041 tcgcttaacg gcacgattat cgtcggtcgt gatattgctc acgccaaact gaaagagcgg
     5101 atggataacg gtgaagggct gccgcagtac attaaagatc atccgattta ctacgcgggt
     5161 ccggccaaaa cgccggaagg ttatgcctcc ggttctcttg gcccaacgac cgccggacgg
     5221 atggattctt atgtcgatca actgcaagcg cagggcggaa gtatgatcat gctggcgaaa
     5281 ggcaaccgca gccagcaggt gacggatgcc tgtaaaaaac acggcggctt ctacctcggc
     5341 agtatcggtg gtccggcagc tgtattggcg cagggaagca ttaagagcct ggaatgtgtt
     5401 gaatatccgg aactgggaat ggaagccatc tggaaaattg aagtggaaga tttcccggcg
     5461 tttatccttg tggatgataa aggaaatgac ttcttccagc agatacaact cacacaatgc
     5521 acccgctgtg tgaaataaac agagccgccc ttcggggcgg tttttttaca tggcacgaaa
     5581 gaccaaacgt ttgttatcaa atggtaaata atgagtgagc taaaagttgc ttaacgaaag
     5641 caaaacagaa agaaaaaatt aatcaggtga ggagcaggcc atgaatacag tacgcagcga
     5701 aaaagattcg atgggagcga ttgatgtccc ggcagataag ctgtggggcg cacaaactca
     5761 gcgctcgctg gagcatttcc gcatttcgac ggagaaaatg cccacctcac tgattcatgc
     5821 gctggcgcta accaagcgcg cagcggcaaa agttaatgaa gatttaggct tgttgtctga
     5881 agagaaagcg agcgccattc ggcaggcggc ggatgaagta ctggcaggac agcatgacga
     5941 cgaattcccg ctggctatct ggcagaccgg ctccggcacg caaagtaaca tgaacatgaa
     6001 cgaagtgttg gctaaccggg ccagtgaatt actcggcggc gtgcgcggga tggaacgtaa
     6061 agttcaccct aacgacgacg tgaacaaaag ccaaagttcc aacgatgtct ttccgacggc
     6121 gatgcacgtt gcggcactac tggcgctgcg caagcaactc attccacaac ttaaaaccct
     6181 gacccagacg ctgagtgaaa aatcccgcgc atttgccgat atcgtcaaaa tcggtcgtac
     6241 ccacttgcag gacgcgacgc cgttaacgct ggggcaggag atttccggct gggtagcgat
     6301 gctggagcat aatctcaaac atatcgaata cagcctgcct cacgtagcgg aactggctct
     6361 gggcggtaca gcggtgggta ctggactaaa tacccatccg gaatatgcgc gtcgcgtagc
     6421 agatgaactg gcagtcatta cctgtgcacc gtttgttacc gcgccgaaca aatttgaagc
     6481 gctggcgacc tgtgatgccc tggttcaggc gcacggcgca ttgaaagggt tggctgcgtc
     6541 actgatgaaa attgccaatg atgtccgctg gctggcctct ggcccgcgct gcggaattgg
     6601 tgaaatctca atcccggaaa atgagccggg tagctcaatc atgccgggga aagtgaaccc
     6661 gacgcagtgt gaagcattaa ccatgctctg ctgtcaggtg atggggaacg atgtggcgat
     6721 caacatgggt ggcgcttccg gtaactttga actgaacgtc ttccgtccga tggtgatcca
     6781 taatttcctg caatcggtgc gcttgctggc agatggcatg gaaagtttca acaaacactg
     6841 cgcagtgggc attgaaccga atcgtgagcg aatcaatcaa ttactcaatg aatcgctgat
     6901 gctggtgact gcgcttaaca cccacattgg ttatgacaaa gccgccgaga tcgccaaaaa
     6961 agcgcataaa gaagggctga ccttaaaagc ggcggccctt gcgctggggt atcttagcga
     7021 agccgagttt gaccgctggg tacggccaga acagatggtc ggcagtatga aagccggggg
     7081 ttaatctgca acatacaggt gcagccgtgg aatgattaaa cgaagcggct gcgcctgtgg
     7141 tttataacgg tgctgcacat tgtcggcatc gtaatttaac aactcaccaa cgtccggcac
     7201 gcccgcgcca ttatccctat taatcagtgc aatcagcggc gtagggcagg cgtgttgtac
     7261 ttgtttttga tctcctttgt accagacgcg ggcaatcggc tgcaccttca ccggacgttt
     7321 gatttttaac ttcgcgttct gtggcagggc agcgatatcc tgatattctc gctccagttt
     7381 tctttgccac tcctcgcgcg tccacggtgc gacactgcgt ggtgatttca ggcttttttc
     7441 cagctgtgcc aggacttcat cacgatgcaa atttttaatg atatgtttat tagcccagcc
     7501 aaaacgtaaa gtggcggggt cgtgcagaac ggtgagcgtg cggtaagcat taagtgtaat
     7561 cagccccggc aaatgacgat gcacccattc aaaacgtgcc gcggagggga gttctgattc
     7621 aaccgtgacg atatgctcga acgtggtctt gagtttattg atgtgctgaa tatgactgac
     7681 taacgctgct tgcgaaaagt tatcgacctg gtaacacaac acgccaggca gacggacagc
     7741 ggccttgctg ctgcgatttt cggactgttg ttgaataaat aaatggcgaa aatgacgcaa
     7801 cgccagcgat tgcgcgtcgt tgccgagatg ttgttttacc tcaatacgat taagcggatt
     7861 atgctcatcc tcttttttta cctccggcaa agagaacacg cgggcaacca atagcttgtg
     7921 ttgctcaaga tgagcggcaa atgcagccag ctcttgttcc atctggcgaa aagtagtgtt
     7981 gagtcggtct acgagatcgt aacgcgccat aatattaacc actttagtta caacatcctt
     8041 attttatagc acaatggtga tgacttgacc accgtgcgca tagtgtcagg cagaggtaaa
     8101 ttgcgggatg ttatgccata acggccagct gaacgagaag cgggcaccac ccagttcgct
     8161 ggtgtcacaa ttaaccgtac cgcccattgc cagtgctata gagtggacaa tggccagccc
     8221 cagcccgcag ccgccggttg agcgatcccg gctgggatcg aggcgaacaa aaggttcaaa
     8281 gatatgttcg cggttttctg gggcaatccc tgggccatca tcctcaacaa ttaatgtcgc
     8341 tctattcccc gacagtagca ggctggtttc aaccgttgag tgacagtagc gcagggcgtt
     8401 attgagcaaa ttatccagca cgcgctccat taagcgcata tccaacgccg cataatggcc
     8461 ttgcgcgagc gtttttatcc gtaccgtttt atcgggcgtt actgcctgaa tgtctgccag
     8521 atgcgttgac agccacaacg gcaggtctgg ttcgctaaga tgaagctcgt tttgtgggcg
     8581 atcgagtcgg gcataagtca gaagctcttc aattaaagct tcaagttgac tgatatcgcg
     8641 gttcaacgcc tgggattcgg cggcgctcag gttatcgctc atctccagtc gataacgcag
     8701 gcgcactaac ggtgttcgca gttcgtgagc gataccgtca ataagctgtt ttttgctggc
     8761 aatcaaggcg ttgatattgt ccgccatctg gttaaatgca acaccaagtc gttcaaagct
     8821 cgaaccctca tcaaagtgga tacgttcatt gagatgccca tcgccaaatc gttgcgccgc
     8881 tgcttccagt tttaacatat cctgccagtg cggacgcatc cagataaaca ccggaaaggc
     8941 gagggaaata gcaataaaag cgatcagggc gatatccagt aatcgcatct gatggaggta
     9001 ataaagataa ggaacaggac caactgccag cacgtagtgg ctgcgcggga tacgttgcaa
     9061 aaacgtgtac tgatcgtcca gggcgacaat ttcgccgcca cgcagccggt gcatggaaat
     9121 atcatcaaga tggtatttac tcagcggctc gacacgcaga tcgaaagaga gatttaaatc
     9181 catctctttc agcgtcttac cccagtcgtg tggggggatc tcacgcaatt cgctgcgcat
     9241 cagatacagc gaactgttca tcaaatcatc cagcgactgt ttgcctgcgc gttcggcggt
     9301 aaatttgtac accagcccga ccagcagaga catcacaagg aagcagacaa acaataacag
     9361 gtaaaactgg ataaacagtt ttttcatcgc ttattcccac gcatgaggcg caaaaagata
     9421 gcctttgtta cgcacggttt taatacgata aggttctgcg gcgttatcga gcagtttttt
     9481 tcttaaccgc gaaatagcca cgtccacgct acgatccaga ccgtcataac tgacgccgcg
     9541 taaatttttc agcaatgcat cgcggtccat gatttgcccg gcatgggtag ctaattccca
     9601 caataattcg aaatcagctg tcgagagcga gatttcagtg ttagccaggg tgactacgcg
     9661 gttgatggga tcgatggtca acgtgccgaa atgcagggct ttgtagggag tcagagacgt
     9721 ttcctgaata cctttggtca ctgtggcttg ctcattctga cgcaaatgca aacgtaaacg
     9781 cgctagcaaa acagcagggg gcgtcgtttt gagaatatag tcgcaggcgc ccatttccag
     9841 tgccaggatg tggttcatat cgctatcgag agaggttaga agaacaatcg gtccggacca
     9901 ctttgcgcgt aaatcacgac aaatggtcat gccgtccttg cctggtagca tgatgtcgag
     9961 taacaccaaa tccggatttt ctcgcagaat agtttcttcg gcccggtcgc cgcgcggctc
    10021 tacggtaacc tgcatatcat gttttgccag gtatgcggca atcagtgcac cgacttccgc
    10081 atcatcttcc acaaatacga tagtgttcat aacattcacg gtagatataa aaacgtcaca
    10141 atacaccgca ctgtttttac ttactatcaa tcgtttctaa acaaggtttc atcaggtatt
    10201 ctactgatga cttgttatgg aagtgttaag gtaaaaagat agggctggta atcaaagccg
    10261 cgctcggtgc gctggtagtg ctgttgattg gtgttttagc aaaaacgaaa aattattata
    10321 tcgccgggct gattccactt ttcccgacct ttgcgcttat cgcgcattat attgttgcca
    10381 gcgaacgcgg cattgaagcc ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc
    10441 cctattttgt ttacctggcg tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg
    10501 cttttgttgg ttctgttgcg tgttggggga taagcgcatg ggtgttgatt atatgctgga
    10561 taaagctgca ttaacgcaga tgacgaccgc catcaagagg gaaactgcgt ccggtgacaa
    10621 agcagctggt aagtaagtaa tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt
    10681 tcatcagtga tttattcagc gcctgttgtc gatattcggc atcatcatgt tcattaaaca
    10741 ggatcagcga tggcgcaata gaattcactt tcacttccgg tgccagcttg cgggcaaacg
    10801 agcgggtcat attatccagt gccgctttgc ttgcagcata ggcaatatgt ttgtcgctac
    10861 cgcgctccac cacataatcg gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac
    10921 gcagtaatct ttccagagca tggttgagca ggtatggggt attaacgtgg atctgcatca
    10981 tgcaagccag tacgtcggtc agtggcgcac ccggtttttc cgccatccac gcgctggcgt
    11041 tatgcaaaat agcacgcaga ccatgggtgc tttttagtac ttcatcggca aacgccatca
    11101 caccgtcgtt ggtcgaaaaa tcagcctgaa tacactgcgc acctgcttta atcagtccat
    11161 caatggcggg atagtgcgtc cgatagctga caatcaccgg ttgcttttga ttaatgaaat
    11221 gccatgcgag ggcgaggccg atgcggcgac ctccgccagt aattaatatt ggcaagggct
    11281 gggttttacc catcgtgatc tcctttgcta tccaacgatg ggatgaacgt tatcccacca
    11341 gcatccaggt cgccggaact gaggcaatta acagcatgcc aatcagcacc atttcctgac
    11401 ggttcagtac attatcatgt gtatgcgttt tacgcgcgta caggaaaacc agcagccccg
    11461 gcgcatacag aacaacggat aacagcaagt gcattggacc agaggcgtac agtaaccata
    11521 agccataaat gcaggcaccg acacctaccg ccttatgcag tggacgggtt gcgattttca
    11581 gcaagaacgc gccgaccagg aaatagggca ccagaatcat ttctgaggcg atggtcagca
    11641 atgtgttgta atcggaaccg gtcagccaga tgagcaccag acaaatttgc acgcagatgt
    11701 tggtcagcca cagtgaagcg gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgcg
    11761 ggaatgcttt atgagtggct gcaaggaacg gaacttctgc tgccatgatg gtccagctta
    11821 agtacgcgcc gcaaacggaa acgatcaaac cggcagcgat gatgatttcg ccccacggtc
    11881 ccatcatttc caccatcaga ccggccattg acgggttacg aatttcagcc agttcaggac
    11941 gttccaccac gcccagcgaa agcagcgtta ccagcaaata aacgcccaga gcggagagaa
    12001 ccgccagcag tgtcgctttg ccaacatcac gcttattacg cgcacgcgca gaaacaacca
    12061 cggcaccttc cacaccaatg aaaacccaca gggtgatcag catggtgttt ttcacctgtt
    12121 cccacacggg tacgccaagt gcaagtccgg tgaagtcgag cttgaaggta tccagtttga
    12181 acatcattat cgccagcaca acaaacagac ccaacggcaa caattttgcc agtgtcgcca
    12241 caaggttaat gctggcagcg gtttgcaccc cgcgcaggat caaaaagtga acaatccaca
    12301 ataaagccga tgcaccgacg atcgattgcc aggtattacc atcgccaaac aggcgcaatt
    12361 ccggcgtgtc cgtaaaaaag cttaacgcgg aaaaaacgat caccagatag gagacgttgg
    12421 cgatgaccgc gcacagccag tatccccatg cggaacaaaa gccgattagc tcgccaaacc
    12481 cttcgcgggc ataggtaaag ataccgccgt caagttcggg gcgaatgcgc gtgaggatca
    12541 gcatggcaaa ggccagcaat aaaatgccag cgccagtaat accccagccg atgagcagtg
    12601 ctgccgggct ggcaactgcc gccatatttt gcggcagact gaaaacaccc gcgcccagca
    12661 ttgagcttaa taccagcgcg gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc
    12721 agtgattaaa acgtgacaag aagaataaat attagggcaa aacgcataaa aatggagttt
    12781 gccactgaag cgcgggattt tacgaagaga tgtgtctgca tgcaatggtc gcagggaaat
    12841 ttatgactaa aaatgcgaaa ggcggcatca agccgcctta taggagtaac tgaatagtta
    12901 tttatacaga tctgcgctga cggtcaggtt attaccacgt tcctgccact ggcgggtgat
    12961 gtggtaatac ttagcacctt tcttcgcggc acgtttcgca acctgatagg agacttcggt
    13021 catgttgccg tagttgccag agaatttgat gctgtcgaac gggaccatca tcgctgctgt
    13081 cgctttgttc agttcttcga ctttagtgcc gtccgggagc gtgacggtgt aacgcccgcc
    13141 ttttgatgac tgggtttcaa agaagcgacc gacttcagaa cttggtgatg cggtagtcgc
    13201 aacacccggg atctcaactt tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc
    13261 tgcttcggaa tctgccggga ttacatccgg gctctggacg atacgtttct tagcatcttt
    13321 tttatagatg aatgcagtaa tacgctggtt accgccctgg ttggcatcga tttgacgaac
    13381 aatgtagaaa gagtaggcac ctttcgcttt tgccgctttg gtgatggcat cattgacttc
    13441 tggctggcta cgatagaagc cctggacggt gaccgtgtca aacggttcaa tcagaacagc
    13501 ctgatctttc ggcagttcga caacaccgtt aattacgcga ttacttgttt cttctgcttt
    13561 ttcagcatcg gctttataga ggtcagcgac cacacgccag ttaccgctgt taccaaaatc
    13621 agaagtgtcg acaacataaa aagaggcggc accttcttta tctgcgcgac gagaaacggc
    13681 tttcaccgct tcgccaatag cattaaaacg accggtaacc actacacggt caaaaggttt
    13741 aaccgctgcc gcttgctccg gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc
    13801 agaaagcagt gccgacgcca ggagggtgtt cttaagcttc ataaaaataa tccttcgcct
    13861 tgcgcaaacc aggtactggt attgttatta acgagaaacg tggctgatta ttgcatttaa
    13921 acggtgtaac tgtctgcgtc atttttcata tcacattcct taaaccaatt ttaattctgc
    13981 tcaaatgacc gtctatgctt aaaaaacagc cgtatcacca tcaatactac tgaagcaact
    14041 gaattgtata agttaattta atgttaagta gtgatttgta ccggggcgat gtctcgtttt
    14101 acccggccgc cgaagacaat tatcagtctt tatccggcgt tctaaggtgt ttatcccact
    14161 atcacggctg aatcgttaat attttgcgag ttcacgccga aatactgatt tttggcgcta
    14221 gatcacaggc ataattttca gtacgttata gggcgtttgt tactaattta ttttaacgga
    14281 gtaacattta gctcgtacat gagcggcttg tgtggctcct gacacaagca aaccatcatc
    14341 aataaaaccg atggaaggga atatcatgcg aattggcata ccaagagaac ggttaaccaa
    14401 tgaaacccgt gttgcagcaa cgccaaaaac agtggaacag ctgctgaaac tgggttttac
    14461 cgtcgcggta gagagcggcg cgggtcaact ggcaagtttt gacgataaag cgtttgtgca
    14521 agcgggcgct gaaattgtag aagggaatag cgtctggcag tcagagatca ttctgaaggt
    14581 caatgcgccg ttagatgatg aaattgcgtt actgaatcct gggacaacgc tggtgagttt
    14641 tatctggcct gcgcagaatc cggaattaat gcaaaaactt gcggaacgta acgtgaccgt
    14701 gatggcgatg gactctgtgc cgcgtatctc acgcgcacaa tcgctggacg cactaagctc
    14761 gatggcgaat atcgccggtt atcgcgccat tgttgaagct gcacatgaat ttgggcgctt
    14821 ctttaccgga caaatcactg cggccgggaa agtgccaccg gcaaaagtga tggtgattgg
    14881 tgccggtgtc gcaggtctgg ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg
    14941 tgcattcgac acccgcccgg aagtgaaaga acaagttcaa agtatgggcg cagaattcct
    15001 cgagctggat tttaaagagg aagcgggcag cggcgatggc tatgccaaag tgatgtcgga
    15061 cgcgttcatc aaagcggaaa tggaactctt tgccgcccag gcaaaagagg tcgatatcat
    15121 tgtcaccacc gcgcttattc caggcaaacc agcgccgaag ctaattaccc gtgaaatggt
    15181 tgactccatg aaggcgggca gtgtgattgt cgacctggca gcccaaaacg gcggcaactg
    15241 tgaatacacc gtgccgggtg aaatcttcac tacggaaaat ggtgtcaaag taattggtta
    15301 caccgatctt ccgggccgtc tgccgacgca atcctcacag ctttatggca ctaacctcgt
    15361 taatctgctg aaactgttgt gcaaagagaa agacggcaac atcactgttg attttgatga
    15421 tgtggtgatt cgtggcgtga ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc
    15481 gattcaggta tcagctcagc cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga
    15541 ggaaaaatgt gcctgctcac cgtggcgtaa atacgcgttg atggcgttgg caatcatcct
    15601 tttcggctgg atggcaagcg ttgcgccaaa agagttcctt ggacacttca ctgtgttcgc
    15661 gctggcctgc gttgtcggtt attacgtggt gtggaatgta tcgcacgcgt tgcatacacc
    15721 gttgatgtcg gtcaccaacg cgatttcagg gattattgtt gtcggagcac tgttgcagat
    15781 tggccagggc ggctgggtta gcttccttag ttttatcgcg gtgcttatag ccagcattaa
    15841 tattttcggt ggcttcaccg tgactcagcg catgctgaaa atgttccgca aaaattaagg
    15901 ggtaacatat gtctggagga ttagttacag ctgcatacat tgttgccgcg atcctgttta
    15961 tcttcagtct ggccggtctt tcgaaacatg aaacgtctcg ccagggtaac aacttcggta
    16021 tcgccgggat ggcgattgcg ttaatcgcaa ccatttttgg accggatacg ggtaatgttg
    16081 gctggatctt gctggctatg gtcattggtg gggcaattgg tatccggctg gcgaaaaaag
    16141 ttgaaatgac cgaaatgcca gaactggtgg cgatcctgca tagcttcgtg ggtctggcgg
    16201 cagtgctggt tggctttaac agctatctgc atcatgacgc gggaatggca ccgattctgg
    16261 tcaatattca cctgacggaa gtgttcctcg gtatcttcat cggggcggta acgttcacgg
    16321 gttcggtggt ggcgttcggc aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc
    16381 caaaccgtca caaaatgaac ctggcggctc tgatcgtttc cttcctgctg ctgattgtat
    16441 ttgttcgcac ggatagcgtc ggcctgcaag tgctggcatt gctgataatg accgccattg
    16501 cgctggtatt cggctggcat ttagtcgcct ccatcggtgg tgcagatatg ccagtggtgg
    16561 tgtcgatgct gaactcgtac tccggctggg cggctgcggc tgcgggcttt atgctcagca
    16621 acgacctgct gattgtgacc ggtgcgctgg tcggttcttc gggggctatc ctttcttaca
    16681 ttatgtgtaa ggcgatgaac cgttccttta tcagcgttat tgcgggtggt ttcggcaccg
    16741 acggctcttc tactggtgat gatcaggaag tgggcgagca ccgcgaaatc actgcagaag
    16801 agacagcgga actgctgaaa aactcccatt cagtgatcat tactccgggg tacggcatgg
    16861 cagttgcgca ggcgcaatat cctgtcgctg aaattaccga gaaattgcgc gctcgtggta
    16921 ttaatgtgcg tttcggtatc cacccggttg cggggcgttt gcctggacat atgaacgtat
    16981 tgctggctga agcaaaagta ccgtatgaca tcgtgctgga aatggacgag atcaacgatg
    17041 actttgctga taccgatacc gtactggtga ttggtgctaa cgacacggtt aatccggcgg
    17101 cgcaggatga tccgaagagt ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc
    17161 agaacgtgat tgtctttaaa cgttcgatga acactggcta tgctggtgtg caaaacccgc
    17221 tgttcttcaa ggaaaacacc cacatgctgt ttggtgacgc caaagccagc gtggatgcaa
    17281 tcctgaaagc tctgtaaccc tgacggcctc tgcggaggcc gtcactcttt gttgagatcg
    17341 cttaacagaa cggcgatgct ttgacctccc gttgtttgtt caagcgcaat tttgacaata
    17401 attgttaacg gcacggaaag cagcataccc accggtccta acaaccatcc ccaaaaaatc
    17461 aacgacaaaa ataccaccaa tgtggaaagc cccagcccac gtcccatgat gcgcggctcc
    17521 agaatattgc cgaagaccag attaattagc agatatcccg ccagcaccag caacgcttcg
    17581 tagaagccat taaacaccag tacctgagcg atagggggga ttgccgcgag aactgaacca
    17641 atattcggga tgtaattaag cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca
    17701 tcgagtgcgg cgagcatcgc ccaggcgacc tggccggtga tgatgctgat ggctgttttc
    17761 agcaccagat aatgagacac actgtcaatc gcacgctgaa tcgccgccat cccttcaacc
    17821 ggacgcgcca tcatttgctg aaattttccg ggcaattgtg gcacttcgag caacataaac
    17881 agcaccgtca gcagcaataa aaagattgat gacatggcat tagataactg cgtcaataag
    17941 ttggtgagca acgtcatcgc cgcgttcgga tcaatgtaat gcgccagctg gtcaactgag
    18001 acatcaatcc ctacgcgttg caacaacggt tcaatcgctt gcagcggcgt cataatagag
    18061 ttgcgatatt gcggtaacgt ccgcgtcaac tcattgagcg cggaacccag ataagctaac
    18121 agcaacacca tcgccatcac gatgatggtc atcaaaattg acactgccag tacacgcggc
    18181 acacgccagc ggaccatgtg ttgcaccagc gggttaagaa taacagcaat aaataatgcg
    18241 agaataaacg gcacgatgat ctcggcggca aaacggatac cgcagagaat aatgaccagc
    18301 attcccaaca taatgacgat ttttaggcca ttgagcgtga tgatcggctt tgccatgctt
    18361 gtccccgttt tttcttcctt tgctaataat aatgtctttt tcgctccgtc atcataacga
    18421 aaatcaaacc gctcaggtaa agaagtgaaa atattttgag ttaattctta agctatgata
    18481 caaatcaggc gtgttcaact accgaggaca attatcatcc gcgaggacga gaagcaacac
    18541 tgcggataat tgtaatatta tggacaattt gttcaggacg ttattttcta catttaccca
    18601 tcttcgtacc tcatccacta tcttgctcgt aggtgagcag cactggcgca acgcgcttta
    18661 gtcctttctt ctgccaaagc ttgcgctaag cgcggggctt gaccacttta ctttggtttc
    18721 gctgaattaa gcgaaaatta aaataattct cttgcaggag aaggacaatg tatatttatt
    18781 ggattttatt aggtctggct attgctacag aaattaccgg tacgctgtcc atgaaatggg
    18841 cgagcgtcag tgagggaaat ggcggcttta ttttaatgct ggtgatgatt tctctgtcgt
    18901 atatatttct ctctttcgcc gttaaaaaaa tcgccttagg cgtggcttat gcgctgtggg
    18961 aaggtattgg tattttattt attaccttgt ttagcgtttt gttattcgac gaaagtttat
    19021 cgctgatgaa aattgccggg ttaaccaccc tggtggccgg gattgtgttg atcaagtcag
    19081 gtacccgtaa agcgcgtaaa cctgaactgg aggtgaacca tggcgcagtt tgaatgggtt
    19141 cacgccgcct ggctggcatt ggcaatcgtg ctggaaatcg ttgctaacgt ctttttgaaa
    19201 ttttctgacg gctttcgtcg caaaatattt ggcttgctct ctctggcggc ggtgctggct
    19261 gcctttagtg cgctttctca ggctgttaaa gggatcgact tgtctgtcgc ttatgcattg
    19321 tggggcgggt ttggtattgc cgccacgtta gccgcaggtt ggatcttgtt tggtcaacgg
    19381 ttaaatcgta aaggctggat tggcctggtg ctgctgttgg ctggaatgat catggtgaaa
    19441 cttgcctgat gaacacgctg cccgcgctgt cgggcagcgt ttgaacatta tttttgcgac
    19501 agttgatcca gcttgtcgcg aaaaccggta acagaaatgg cccggttatc ggcgcgccag
    19561 cgatctttcg cggcaggagc cgagctttgc accccaatta attgccagcc gtcatcggta
    19621 tgcaacatca gaggcgaacc gctgtcaccc ggcaaggtat cgcactgatg cgacatcacc
    19681 gacgtttgcg cccagccagt cacttcacag ttttgatgac tgtacaacgt atcgagatga
    19741 tcttcagggt agcccgcctg agtcacttta cgaccagccg cttttaatgc ggcggtaagc
    19801 gcggctttat ctccctcaaa taacggcaac ggtgtaatgc cagaaggggg attacgtagc
    19861 acaatcaaac cgaagtccca gggcgcagct gcgggaggta caatccagcc atccccatct
    19921 gcttttaacc gctttcccag tgtcggatca acgcggcctt ctatgtcgtg gatctcataa
    19981 cgccaaagac ctttatttga cacgaaacgc agcgctaccg ctttatcggc tttacccttt
    20041 ggcggcgtca ataaacagtg tcctgccgtt aatgccagat tgggtgcaat cagcgtcgcc
    20101 gtacataaat tgccgctggc cgtttccagt tggccaaccg catcccacgg tgattgggtc
    20161 gtgtcattca ctggcacacg atcatcatga ccaaaaaaca gggtgctgac ctcatcgttt
    20221 gccgatttgg caacgtctgg tttatctgca aacacaaaag cagacgtcaa actaattgca
    20281 cccaacacta cagcaatggt tgtacgcata tcacactctg gtgggtaatt atgattatta
    20341 aaagcaaacc ctcataaata ctatagacgg gacgggatga aagtgggagt aaaatcagat
    20401 aactacgatc aggaaagata aaaacgggcg gcaatcagtg cgcataaaat aaggatgata
    20461 agaatgaact caaagcgata acggcccacc atactaccct ccgctaaagg cggtgctaaa
    20521 cgttacccgc ttagcaccgt aattttcagg cgcgaggggg cgcgccagca ttactgttga
    20581 aaacttacgc tgcgggttgt gcagcaggtt ttgccggttg ctgatggctg tgtttcttgg
    20641 cgtgtttctt cgccgcctgc gctttttgtt caggggcttt ctgttcagct ttggcatttt
    20701 tatgatgctt tttagccgcc tgcgctttct gggcaggtgc tgctttatgc tgttttttat
    20761 gatgtgtggt tttcgcaggc gctgctttgg tggtcgtcgc agtcggagca ggtgtggtcg
    20821 cagtctctgc agcaaaggca gcagaagaca gacccatagc agcggcaaca accagagcta
    20881 atactttttt cattgtcata ccctcaattt gttttttcat ttaaccccac tgcggggccg
    20941 ttgaaataac tatatccctg ggaatggcag acttccgtga gtggttggtt tcagcgtgta
    21001 acgatatgta caaacgctga atagattaca gcattgataa taacgtattg tgacttaagg
    21061 aaaatttagc taccaataat agtagtcttg atcgtgttaa ctattttact acttacaaag
    21121 cggtagaata actgcgcatc agtaataaat gacacacagc aaaatgaatc cgtttatttg
    21181 ggtacttata atcctgatga cgctagatgc gctgcgggaa ttggctggcg cttcgtctat
    21241 tttaggatgg ctattaacgt tggtttgagc aggcaataag tccggacggg gatttacctc
    21301 agtccggact tattttttca ggcgtgcaga cgatgatgca aacgcgtccc gaccagcaga
    21361 gcaatgacca gcatcagagc aataaatgcg ccgacgccgt tccagccata gttatgccag
    21421 aaaacaccac ccagcgtccc ggcaatactc gaccccagat agtaactgaa cagatacagc
    21481 gaggaggcct ggcctttagc gcgttttgcg cgggggccga tccagctgct ggcaacggag
    21541 tgggctgcga agaatccggc tgagaagagt aacattccgg caaagatcag ccacagcgag
    21601 ctgaataagg tcatcagtaa accaaacagc ataacccccg tcgaaaacaa catcaccgga
    21661 ccgcgtccat agcgggtggt catggttccg gctttgggtg agctccatgt accggtcaaa
    21721 taagccagcg ataataagcc aaccacggcc tgactgacat gccaggggga aagcatcaac
    21781 ctatagccga tgtaattaaa cagcgtgacg aacgacccca tcagcaaaaa gccttctgcg
    21841 aacaataacg gtaatccccg gtcacgccag tgcagacgaa agttgataaa taacgtctta
    21901 gggcgcagag aagtcgggcg aaaatggcgt gattcaggga ggattttcca gaacatcaac
    21961 gccgaggcca gcgcgaaaca accgattgcc gccagagcaa ttcgccagtt gaaaaagtcc
    22021 gtgaagacac cgctaattaa gcgtccgctc atgccgccaa ttgagttgcc gctgatatac
    22081 aaccccattg aaaaggccac gaaactgggg tggatttcct cgctaagata agtcatgcca
    22141 acggctgcca ccccacttaa cgaaagccca atcaaggcgc gcataatcaa aatgtcgtgc
    22201 cagctggtca tcattgtcga aagtaacgta caaatggagg ccaacagtag cgccgtgacc
    22261 atcactggtt tgcgaccaat ggcatcggat agcgggccag taaacagcaa accgatagcc
    22321 aacatcgccg ttgaaatgga cagtgaaata ctactgttcg cgggtgttaa gccaaactcc
    22381 tgcgaaagca ccggaaggat aggttgcaca caatagagaa gtgcgaaggt tgccagtccg
    22441 gcagagaaaa gcgccagggt gacgcgcata aattgcggag taccgcgttt aataaattga
    22501 tttggctgag aaatgctttg cttgtcagtg tcgcttgccg gagcgccatc aacagttgta
    22561 gtacggctca cttgaaatcc ttgctaaata tgcctgtgga tcaggcttat gaatagatta
    22621 ggaaaatcga attgttctgt ctaatatatt aataatctca aataagatgc tttaaatatg
    22681 aatattgaac ttcgtcatct gcgttacttt gtcgctgttg cagaagagct gcatttcggg
    22741 cgtgccgctg cccgcctgaa tatttcgcaa ccacctctaa gtcagcagat tcaggcgctg
    22801 gagcaacaaa ttggtgcccg actgctggca cggaccaatc gcagtgtatt gctgacggaa
    22861 gcaggaaaac agtttcttgt agatagtcgg caaatcctgt ctatggtgga tgatgctgcc
    22921 gctcgcgctg aaaggctgca tcagggcgaa gcgggggagt tgcgcattgg ttttacttcg
    22981 tccgctcctt ttattcgggc ggtgtccgat acgttatcgc tgtttcgccg tgattatccc
    23041 gatgtccatt tacaaacccg cgaaatgaac actcgcgagc aaatcgctcc gctcattgaa
    23101 ggaacgctgg atatgggatt gctgcgtaac acagcgttac cggagacgct tgaacacgca
    23161 gtcatcgtcc atgaaccgct tatggcgatg atcccgcacg atcatcccct ggcaaataac
    23221 ccgagtgtaa cgctggctga actggcgaaa gaaccctttg tcttttttga tccgcacgtc
    23281 ggaacagggc tgtatgacga tattctcggg ctgatgagac gttacaattt gacgcccgtc
    23341 atcactcagg aggtgggtga ggcaatgacc atcatcggtc tggtttccgc cggtctgggt
    23401 gtttcaattt tgcctgcgtc atttaaacgt gttcagctca acgaaatgcg ctgggtgacg
    23461 attgctgaag aggatgcggt ttctgaaatg tggttggtct ggccgaaaca tcatgaacaa
    23521 agtccagctg cgcgaaactt tcgtattcat ctgctgaatg ctctcaggtg agggaaattt
    23581 cagcgaaaaa gcccgaaaaa tgtgctgtta atcacatgcc taagtaaaaa tttgacgaca
    23641 cgtattgaag tgcttcacca tagcctacag attatttcgg agcgcgaaaa tatagggagt
    23701 atgcggtggt tgctgaaaat cagcctgggc acattgatca aataaagcag accaacgcag
    23761 gcgcggttta tcgcctgatt gatcagcttg gtccagtctc gcgtatcgat ctttcccgtc
    23821 tggcgcaact ggctcctgcc agtatcacta aaattgtccg tgagatgctc gaagcacacc
    23881 tggtgcaaga gctggaaatc aaagaagcgg ggaaccgtgg ccgtccggcg gtggggctgg
    23941 tggttgaaac tgaagcctgg cactatcttt ctctgcgcat tagtcgcggg gagattttcc
    24001 ttgctctgcg cgatctgagc agcaaactgg tggtggaaga gtcgcaggaa ctggcgttaa
    24061 aagatgactc gccattgctg gatcgtatca tttcccatat cgatcagttt tttatccgcc
    24121 accagaaaaa acttgagcgt ctgacttcga ttgccataac cttgccggga attattgata
    24181 cggaaaatgg cattgtacat cgcatgccgt tctacgagga tgtaaaagag atgccgctcg
    24241 gcgaggcgct ggagcagcat accggcgtac cggtttatat ccagcatgat atcagcgcat
    24301 ggacgatggc agaggccttg tttggtgcct cacgcggggc gcgcgatgtg attcaggtgg
    24361 ttatcgatca caacgtgggg gcgggcgtca ttaccgatgg tcatctgcta cacgccggca
    24421 gcagtagcct cgtggaaata ggtcacacgc aggtcgaccc gtatgggaaa cgctgttatt
    24481 gcgggaatca cggctgcctc gaaaccatcg ccagtgtgga cagtattctt gagctggcac
    24541 agctgcgtct caatcaatcc atgagctcga tgttacatgg acagccgtta accgtggact
    24601 cattgtgtca ggcggcattg cgcggcgatc tactggcaaa agacatcatt accggggtgg
    24661 gcgcgcatgt cgggcgcatt cttgccatca tggtgaattt atttaaccca caaaaaatac
    24721 tgattggctc accgttaagt aaagcggcag atatcctctt cccggtcatc tcggacagca
    24781 tccgtcagca ggcccttcct gcgtatagtc agcacattag cgttgagagt actcaatttt
    24841 ctaaccaggg tacgatggca ggggctgcgc tagtaaaaga cgcgatgtat aacggttctt
    24901 tgttgattcg tctgttgcag ggttaacatt ttttaactgt tctaccaaaa tttgcgctat
    24961 ctcaatttgg gccaggaaag cataacttag actttcaagg ttaattattt tcctggttta
    25021 tatttgtgaa gcataacggt ggagttagtg atgctgaagc gtttctttat taccggtaca
    25081 gacacttctg tagggaaaac ggtggtttcc cgcgcattgc tacaagcgtt agcctcccag
    25141 ggaaaaacgg ttgcgggata taaacccgta gcgaagggga gcaaagagac acccgaaggg
    25201 ctgcgtaata aagatgccct ggtgttgcag agtgtttcaa ccatcgaact gccttatgaa
    25261 gcagttaatc ctatcgcgtt aagcgaagaa gaaagtagcg tggcgcacag ttgcccaatc
    25321 aattacaccc tcatttcaaa cggcctggca aacctgaccg ataaagtcga tcatgtggtg
    25381 gtagaaggga ctggcggctg gcgcagtctg atgaatgatt tgcgtccgct ctctgaatgg
    25441 gtggtgcagg aacaactgcc ggtgttgatg gttgtcggta ttcaggaagg ttgcattaac
    25501 catgcactgc taacagctca ggcgatcgcc aacgacgggc taccgctcat tggctgggtg
    25561 gctaaccgaa tcaatccagg actggcgcat tatgcggaaa tcattgatgt gctcggtaaa
    25621 aaacttccgg caccgctcat tggtgaactg ccttatctgc cgcgcgccga acagcgtgaa
    25681 ctggggcaat acatccgctt agctatgctt cgcagtgtgc tggcggtaga tagagtcacg
    25741 gtgtaacgtc cgcgaaatta ccgacgcaat tacgcaggca atcaataaac cggggagtag
    25801 ctgatactcc ccggtcattt cacatatcat caacgtcgac ataatcggcg cgtgcgtggt
    25861 cgccgccaac agtgtcgcca tcccggtcaa tccgagtaaa agcgtaattt cttcgccatc
    25921 agggaaccat aatcccaggc tacgaccata caacatgcca atggcaagac cgataaatag
    25981 cgtcggtgta aagacaccac cgggcgcgcc agaaccacta ctcgccagca cggcaaacag
    26041 tttacagagg aagatcccgg caatgatcat taacagtggc ggggcggtta aaaaggattg
    26101 tacggtgcta tagccgttgc cccacactgc aggtgtgaac agggaaagca gacccacgat
    26161 caacccgccc agtgccagtt gccagggcgg cgcaagtttg agacttacaa atccacgatg
    26221 acaggcgttc attaacgtta acaacagtgg tccgcacaga cctgccagca cacctgtact
    26281 gataatcagc gcatagtcac gagcctgaac cgtcactgag agttgtacgc tgtagagtaa
    26341 tgcgtcgcta tgattaataa gattgctaat cagcaacgcc acgacggcgg aaataatcac
    26401 cgggccgaga gaggccaaca tcatagtgcc aaacagcact tcggctataa ataaactgcc
    26461 agcaagcggg gcgcgatagg ccgcagccat tcccgccgca gctccacagg cgatccataa
    26521 tttccactcc tggcgtggcg taaaacgttg ggcaaaacac gaggctgcaa gggcagctaa
    26581 aagaatcatc gcaccttcgc gaccaattgc actgccgctg gttactacca gtagagaggc
    26641 aagcgattta accaggcttg ctgcgtaatc aaactgacca tcggtttgca acgcttccat
    26701 gtaatcggtc ggcgcatgag ggcgttgctg ggtaaatttc tgccagccca tcagcaacaa
    26761 acccgccgcc agtccgccga gcgccggggt tagcagccgt cgccacgggg aaagatttgt
    26821 cgctgcattg accagactgc cggagtcatt attgaggaac agccactcca gtagcagcat
    26881 cgcatgacga aacccggcaa cggcaaaggc cgcaagaata ccgacgattg ttgcgataag
    26941 cagacggcgg aacatagtgc gcagatccgg atagatgtgt agatgatgca tgagcagaaa
    27001 tgggcaatga aacacgggcg actattttgc ggcaaaagag gccagccgca aaagcagtgc
    27061 gtgctttaag agataaaaaa aacgtgacac tgtaacctat taattgccga attgcgtgta
    27121 atcgtcacca tccggcaata atacggtgat cctatcgaaa cagcggttta accgcgacag
    27181 gaatcaacag ttgtgattgc cattgcgcca gcgttaagcg agcgagttca cccagtgcac
    27241 ggtagaaagg gtgttctgct ttttcgataa aaacgtcaag aaaacgtgtt gaccacggaa
    27301 aaaggtgcca tgccagcaat tcttcgcatt ctgtctggcg accattctcc gccaaccacg
    27361 cagccatcag cagcagtgaa ccaaaatgat cttccggttc gttttgcttc atttcgaact
    27421 gaatgccttt ctcgcgcatc cactgacgaa gtgccaatgt tgaatcgcca aacagcacag
    27481 attcgcgatc cagccagacc gatccccacg gcggagacgg cagtgcccac gggccgacaa
    27541 acaaacgctg ccaggcctgg gcgtgagtct cttcactctg tgtctgaaaa gccgtcacca
    27601 gcggcgctaa tgacgcttct ggtagaggcc actgagtttc ccagccgtca cgggtgagta
    27661 ccgcaaccag aggtgcggct tctgcactct ctggagcgta ataaaacagc gcgcccaaca
    27721 cgcgcgccgc aacagaaaaa ttatcttgct gtgaaaaatg ggtcattcct aacatcctga
    27781 aatgtgcggg ttgccccgca ccaatttgtt aacctgcaat agccattcct accgtcatgt
    27841 gcaggcaata aaagagcaca cgcccgatca tctcaccgcc aagaatgagg attaacccca
    27901 taacaagtcc ggcaacgtga ggttcacggc gacgaatgag cggacaaagc cagcatccaa
    27961 gacctgcgca taacaatacc acacgccaga cctgtaagga ggcgtaatct ggtaccagtg
    28021 cgctggcttg ttgtacggat gagtgaatag acgccagaga caaaccttgc agcacaatca
    28081 acccggcaca agcgatcagc gccagaatgc taatgatggc aaatggtgtg gtattaaatg
    28141 taacgcgggc tgcacgtaga attgtggcag caagtattgg accgctcaac aataccgtca
    28201 ggaagaaagc cagcgtagtg taaccgttat gccaggttgg cacagtgtcg atttgataca
    28261 cgcaggtcat catccagacg aaaatgacgc cgagcgccat gctgacaagt aaccagagtt
    28321 tccccaacgc ttgcggcatt ttaccgatga ccgccaccag ccaccataat ccgccaacgg
    28381 caaaaaatat ggaaccggca gcaatctcgt tactcagacc agaagctccg attctattca
    28441 gcgagttaaa cgcccgcagt ggcgatccca ggtgcatgac agaggcaata aacccgatgc
    28501 ccatcaacag ccagaggaaa aacatgccac gaataatgtg ctgacggtct gcgtcatttt
    28561 tggcggcgaa ccagccaatt ccgctaacaa tcagcgcgcc tactacgcat tgacccaata
    28621 cggtaaagat aaccagtggc cattcatgcc atccatttcc cattttacac ttcctccgga
    28681 ttagccagat aaccagtggt atcgccggtc gggcggctgt tggcgttagg tttgataacg
    28741 atattgggtt ttgtgaaatg cgcgcgcggc agtggcgcga cggcagccag agtgccgtgc
    28801 ttctggcgca attcttcaat tggaccgaac tcaagtgcac gcagtgggca ggattcgaca
    28861 catatgggtt gtttcccctc ggcgacgcgc gaataacaac catcgcactt cgtcatgtgc
    28921 cctttttcag cattgtactg tggcgcgccg tacggacaag ccatgtggca gtagcggcag
    28981 ccgatacaaa catcttcatc aaccaccacg aaaccatctt cacgcttatg cattgcaccg
    29041 ctggggcaga cttttgtaca tgcggggtca tcgcaatggt tacaggagat ggagagataa
    29101 taagcgaaca cgttctggtg ccagacgcca ttatcttcct gccagtcgcc gccagcatat
    29161 tcataaatac ggcggaaact gacttccggg ccaagatctt taaaatcttt gcacgccagt
    29221 tcgcaggttt tacagccagt acagcggctg gaatcgataa aaaatccata ttgtgtggtc
    29281 atgggctact ccttaaacct tttcgatctg gacaagattg ctgtgcgacg ggtttccctt
    29341 tgccagcggt gaagggcggt gagaggtcag aatattgata ctgccgccgt gatcgacccg
    29401 gtcaccaaac atatccgctt taagccaagc accttgcccg atggcggtaa cgccaggcag
    29461 aatacgcgga gtcacttttg cggcaatcag catttctcca ttattgttaa atacccgcac
    29521 ggtatcgcca tgacggatac cgcgtgcctg agcatcaatg gggttgatcc acacctcttg
    29581 tgggcaggcc tgctgtaata catcaatatt gccgtaactg gagtgggtac gcgctttgta
    29641 gtggaagccc gttaactgca gtggataagt tttccgcagg ggatcgtccc agccatcaaa
    29701 acctggcgta taagctggaa gaggatgaat aatttcatct tttttcaatt cccaggtatc
    29761 cgcaatcttc gccagtcgtt cagaataaat ttcgattttt cccgaaggtg ttttcaatgg
    29821 gtttgcctgt ggatctacac ggaatgcgcg gaaagcgacg tagtgttctt ccgggcattt
    29881 tttcttaaag atcccggtcg ttttcatctc ctcgtagtcg ggcatctcag ggttacgttc
    29941 cttcgttttc gcatggagat atttgatcca ttcatgctga ctgcgacctt cagtaaaggt
    30001 ttgataaacg tctggtccta agcgtttggc gacttcactc aggatccagt aaattggttt
    30061 gcgttcgaat ttttcgctgg tgacaggctg gaggaaaatg agatatccca tgttaccggc
    30121 gtagtcgtta ggaataatat cttcctgctc aacggtcatc aggtctggca gcagaatgtc
    30181 ggcatatttt gccgatgagg tcataaagtt ttcgatgacc acaatcattt cgcatttcga
    30241 ttcgtcctgc agaatttcat gcgttttgtt gatgtcagaa tgctgattaa ccagggtatt
    30301 tcccgcgtag ttccagatga acttaatggg cacatctagt ttatctttgc cccggacgcc
    30361 gtcgcggatt gccgtcattt gcgggccatg atcgatagca tctgtccagc tgaagcagga
    30421 gattgacgtt ttgaccggat tatccagcac cggcaagcgt tcgatggtaa tggtgtaggt
    30481 cgattcacgc gcaccactat ttccgccgct gatgccgaca ttgcccgtca aaataggcaa
    30541 catagcaata gcgcgtgccg tcagctcgcc gtttgcctgg cgttgcggcc cccagccctg
    30601 gcagatataa gtgggttttg ctgtgccaat ttcacgcgcc agtttgatga tacggtctac
    30661 cgggatacct gtaatttgcg aagcccactg cggcgttttc gctgttttat catcgccttc
    30721 accaagaata taggctttat agtgaccatt tttgggtgca tctgcgggta aggttttttc
    30781 gtcatagccg acgcagtatt tatcgagaaa aggttgatca acgagatttt cgttaatcaa
    30841 tacccaggca atacccgcaa ccagcgcggc atcggtgccc gggcgaatag gaagccattc
    30901 atcttcacga ccagcagccg tatcggtata tcgcggatcg ataacaatca tttttgcgtt
    30961 cgatttctcg cgtgcttttt caagaagata agtgatgcca ccgccgctca tgcgggtttc
    31021 agccgggttg ttaccaaaca tcacgaccag cttgctgttt tcaatatccg tggtgctgtt
    31081 gccatcatta ctgccgtagg tgtagggcat ggcacaggaa atttgcgcag tgctgtagga
    31141 gccatactga tttagtgaac cgccgtagca gttcatcagg cgtttgaccg ccgaggctga
    31201 tggagaagag cgggtcatat tgccgccaac aatccccgaa gagtactgaa tatatacagc
    31261 ctcattgcca tattgtttga cggttttttt caggctactg gcgatagtat ccagggcttc
    31321 atcccagcta atccgttcga atttgccttc gccgcgtttg cccacgcgtt tcattgggta
    31381 attcaggcga tcgggatgat taatacgccg acggatggag cgaccgcgca aacaggcgcg
    31441 tacctgatgg ttgccatact cgtcgctgcc ggtgttgtca gtttccaccc aggtcacttc
    31501 attatcttta acatgcagac gaagtgcaca gcggctacca cagttgacgg aacaggcacc
    31561 ccagaccact ttttcgttgg cctgttgtac cgctgcttct gcactgcgca gggtaaacgg
    31621 caaagaaaaa ccgcctgcag ccagcgccag agaacctatc gcggtagatt taacgagtgt
    31681 tctgcggctg atgcccacca tttgttcatt tttggacata actcactccc tgttctttat
    31741 cgttatataa atgtttatat attgaatatt tagcgcgcta acaatagaga gagtctaccc
    31801 atttggggtt aataattatt aatccatatc aatagaaggg tatgagtaat aaggtgggat
    31861 tatgttgtat gttcaaatcg ccggatgtgt cgtatccggc gttcagtcga taatgtgtta
    31921 ctgcggttca gcaggcgcgt tatcctgggt agactgcgcg ggagcagaga cgttaccgct
    31981 ggtggtacgg gtgtagagaa ttttatgcgt atcattagcg caatgcccga cgacctggga
    32041 atcaggctga tcaacctggt cattgggtac aatacttaac gtgaagctgc tttcgggtac
    32101 gccattattg ataatgcgct gtgatatatc gctctgtatg cgctcacagg atcccggcgc
    32161 ggcgagtacc gcgggtgagg cgagggcgaa cagaagcgcg gcacagcagg ttgagagttt
    32221 catcataagc tccttacgcg aagataactt ctttaagcat agcatttaac gtgtaaagta
    32281 ctgtatttgc tactatgatt gagaatcatc tctactctca ggtgactgtt gtgaaataca
    32341 aattactgcc atgcttactc gcgatattac tcacaggatg tgaccgcaca gaggtaacac
    32401 tttcatttac cccggagatg gccagtttct ctaatgaatt cgattttgat ccgctgcgtg
    32461 gtcccgtaaa agatttcact cagacattaa tggatgagca aggtgaagtg acgaaacgtg
    32521 tttcggggac tttgtcggaa gaaggctgtt ttgattctct cgaattactg gatctggaaa
    32581 ataataccgt ggttgccctg gtactggacg ccaattatta ccgtgatgcc gagacgctgg
    32641 agaaaagagt tcgtttgcag ggaaaatgcc agctagcaga attaccttct gccggggtga
    32701 gttgggaaac cgatgataat ggtttcgtga ttaaagccag cagcaaacag atgcagatgg
    32761 aatatcgcta tgatgatcag gggtatccgc tgggtaaaac cacgaaaagt aacgataaaa
    32821 cattatctgt cagcgccacg ccatcaacgg acccgatcaa aaaattagat tacacagcgg
    32881 ttactttact gaataatcaa cgggttggaa atgtaaaaca gagctgtgaa tatgacaatc
    32941 acgccaatcc ggtggactgt cagctaatca ttgttgatga aggagtaaaa cccgccgtcg
    33001 aacgggttta caccatcaaa aatacgatcg attattatta atactattgt gcggtcggct
    33061 tcaggagagt ctgacccggt gttttgtgct cagccagata ctgatgctgg aatatacaca
    33121 tgcgaatggc attacgatat tgaccattaa taaagaactc atgcatcaat tcaccttcaa
    33181 ccgtaaagcc aagcttgcgg taaatgtgaa tcgctttttc attctcttta tcaacgatca
    33241 gatacagctt atagagattg agaacggtga agccatagtc cattgctaat ttggcggcac
    33301 gggttgccag acctttcccc tgatactccg gggagataat tatctgaaat tctgcgcggc
    33361 gatgaacatg gttaatttcc accagctcca ccagaccggc tttttcgccg tcacattcca
    33421 ccacaaagcg ccgttcgctc tgatcgtgaa tatgcttatc atacagatca gagagttcaa
    33481 caaaggcttc gtagggttcc tcaaaccagt aacgcatcac actggcgtta ttgtcgagtt
    33541 gatgtacata gcgtaaatct tcacgctcca gcgggcgtag cttaacactg tgggcgcttg
    33601 gcataacgtg tccttacatt ccttaaatca ataacaggtt aggggataat aacgcggcca
    33661 gttcgacggt ccaggcagcg caaagtattt ggctcccagt aggcattgat gttggcgctt
    33721 tgctcacatt tatcgcggtt atcaaaagca gcgtcggctt tatcccactc tttttcagtg
    33781 cgtttattca ctttctggcg cagattacgc gtgtcattcc attgctcttt ttccatagcg
    33841 gcgcgctggc ggctttgtgc actgtcgcca gactcaatca ccagtttgtt agtttcggca
    33901 tgaacagttg tgctcaatgc cagtgcgcaa ggcagcagaa aagcgagcag gccgattcgt
    33961 ttgctgagag tgattttcat aattcattcc ctgtatgaat gattaaagct gattctacac
    34021 catccactgc gaacgcaaaa cgttcaagga gggtgtttat attgatgata ttatgtcgcc
    34081 ctataactat acgtgatgtc aataagtgac aaagatgatt aaaacaacgt tactattttt
    34141 tgctactgcg ctgtgtgaaa ttattggatg ctttttgccc tggctgtggt tgaagcgaaa
    34201 cgccagtatc tggctgttgc ttccggcggg gatttcactg gcgctgtttg tctggttgtt
    34261 aacgttgcat cctgcggcga gtgggcgtgt ttacgcggct tatggtggcg tttatgtctg
    34321 cacggcgttg atttggctgc gcgttgtgga tggcgtgaaa ctgactcttt atgactggac
    34381 gggggcgtta attgcgcttt gcggcatgtt gatcattgtt gcgggctggg ggcgcacgta
    34441 ggaacataaa tccattttat caataagata cgaggaagtg tcagctgaca aaaggtattc
    34501 tgtttcatct tttgtcaacc attcacggcg caaatatacg cctttttttg tgatcactcc
    34561 ggcttttttt gatcttcatg cttgtatggt agtagctcag ttgcgtagat ttcatgcatc
    34621 acgacaagcg atgcaaggaa tcgaacatga agatcgtaaa ggctgaagtt tttgttacct
    34681 gtccggggcg taatttcgtc acattaaaaa tcaccactga ggacggtatt acgggccttg
    34741 gggatgccac cctaaatgga cgtgagcttt ccgtggcctc ttatttgcag gatcaccttt
    34801 gtccgcagct tattggtcgc gatgcacacc gtatcgaaga tatctggcag tttttctata
    34861 aaggtgctta ctggcgtcgc ggtccggtta cgatgtcggc catttcagcg gttgatatgg
    34921 cgctgtggga tattaaagcc aaagctgcca acatgccgct ttaccagtta ctcggcggcg
    34981 cgtctcgtga aggggtgatg gtttattgcc ataccaccgg tcacagtatt gatgaagctc
    35041 tggatgatta tgcccgtcat caggagctgg gattcaaagc catccgcgtg cagtgcggaa
    35101 tccctggtat gaaaaccacc tacggcatgt cgaaaggtaa aggtctggct tatgaacccg
    35161 caaccaaagg acagtggccg gaagagcagc tgtggtcgac ggagaaatac ctcgatttca
    35221 tgccgaaatt gtttgacgcg gtacgtaaca agtttggttt tgatgaacat ctgctgcatg
    35281 acatgcacca tcgcttaacg cctattgaag cggcgcgctt tggtaagagc attgaagatt
    35341 atcgcatgtt ctggatggaa gacccgacgc ctgcggaaaa ccaggaatgc ttccgtctca
    35401 ttcgccaaca taccgtcaca cccatcgcgg tgggtgaagt cttcaacagc atttgggact
    35461 gcaaacaact gattgaagag caactcatcg attatatccg taccacgctg acccatgcag
    35521 gcggaattac cggtatgcgc cggattgccg attttgcttc gctgtatcag gtacgtactg
    35581 gctcacacgg tccttccgat ttgtcaccag tctgcatggc tgcggcgctg cactttgatc
    35641 tgtgggtacc caatttcggt gtccaggaat acatgggtta ttccgaacaa atgctcgaag
    35701 tcttcccgca caactggact ttcgataacg gctatatgca tccgggagaa aaaccgggtc
    35761 ttggcatcga attcgatgaa aagctggcgg cgaaatatcc ctatgaacct gcttatctgc
    35821 cagtcgcacg tctggaagat ggcacgctgt ggaactggta aggagtaagg taatgaaaag
    35881 cattttaatt gaaaaaccga atcaactgtc gattatcgaa cgtaaaatac ccaccccgtc
    35941 agcgggtgaa gtacgagtaa aagttaaact tgccggaatt tgtggttcag atagccatat
    36001 ttaccgtggg cataatcctt ttgcgaaata tccgcgcgtc attggtcatg aattctttgg
    36061 cgtcattgat gcagtgggtg acggcgtgga aagcgccaga gtcggtgaac gcgttgctgt
    36121 cgatccggtg gtcagctgtg ggcattgcta tccgtgctct ataggtaagc cgaacgtttg
    36181 tacgacactt gctgtattag gtgtgcacgc tgacggtggt ttcagtgaat atgccgtggt
    36241 tccggcaaaa aatgcgtgga aaattcctga agcagtggcc gatcaatatg cggtgatgat
    36301 cgaacctttt accattgcgg ctaacgttac cggtcatggt caaccgactg aaaatgatac
    36361 cgttctggtt tacggtgccg gcccaatcgg cctgacgatc gttcaggtat taaaaggcgt
    36421 ctataacgtt aaaaatgtga ttgttgccga tcgcattgat gaacgactgg aaaaagcgaa
    36481 agagagcggg gcagactggg cgattaataa cagccagaca ccgcttggcg agagtttcgc
    36541 tgaaaaaggc atcaagccga ctttaattat cgatgccgct tgtcatcctt caatcttgaa
    36601 agaggccgta acgctggctt ctccagcggc acgtattgta ttgatgggct tctccagtga
    36661 accgtctgaa gtgattcagc aaggaattac cggaaaagaa ctctctattt tctcttcacg
    36721 cttaaatgca aataaattcc cggtcgttat cgactggtta agtaaagggt taattaaacc
    36781 agaaaaacta attacccata catttgattt ccagcatgtt gcggatgcca ttagtttatt
    36841 tgaacaggat caaaaacatt gctgcaaagt cttactcact ttttctgaat aataccaata
    36901 acggcgagta agtagtacgc atcttacctc ttttttagag ataaccatta tgacaataga
    36961 aaaacacgaa agaagtacta aggatttggt gaaagcagca gtatcgggat ggttgggcac
    37021 tgcgcttgaa tttatggatt tcaagagtca tgaatagtaa ctatttgata attaataaat
    37081 taattttgca ccacctcgtt ttggggcatg gttggggcaa attcgcttag ctgtgtattt
    37141 aacagggcta cctgtgcatt attgttttca gacatccatt tcccgtatac ctgaaacacc
    37201 atttgcgcat ctgcatgtcc catctggttt gctatgaatg ccgggttggc accggctgtc
    37261 agcgaccagc aggcataagt atgtctcgac tgatatgatt tgcggtggcg gaggccagcg
    37321 cgctttatcg ctgcgtccca catctgcctt attgagtcaa cggtaaaatg gtcaccataa
    37381 ttttttactc tcgctgacac ttcaggttga aaaacaaagg tgcatttttg tttttctgtt
    37441 ctgccgaact ctctgaggtg aacgtcaata atatgttctt tgctcagcct cgttagtgtc
    37501 atctgactcc tgagagcgtc gattgcaggc ttgataaggt gaattacccg attggtacca
    37561 gcctgtgttt tcggtaccgt aaaacgatct tttgctaaat ttctcctgat catcattgtt
    37621 ccatttttca gatctatatc ctcccaccca agcgcacaca gttcaccagg gcgaatgcct
    37681 gtataaacag aaacacacca taaatttttg gcctgctgat ttctgcaggc gtcgataaga
    37741 cggataaatt cctcccgtga aagaggatca ggaatggttc ttgattcctt taatggcgag
    37801 atccccttaa acggattatc tgccaggtaa ccgttatcaa caccgaactg gaacacggcg
    37861 taaagatttg tcatgtaatt atttacggtg acagccgatc tccccggttg cgtaacaata
    37921 taattacttt tggggatctg gtatccagta agtaattctt tacgtaccgc cagcaatttt
    37981 tctttattaa tcgacgaggc aagatttttt tcaccgatta tgctcagaat atttttgatg
    38041 acggcacggt acgtgttgag tgacgttttt gcgacttccg tttctttcag tgccagaaat
    38101 ttttcagcca gttcctttat ggttaaatct tgtcgggcct caccaaattt ttccagattg
    38161 tgtgaggagg gaaactgttt tgcatagtcg aaaacgccgg tttttattgc ataacaaaca
    38221 gaggcgcgca gctcacctgc aacgcgcctg ttttttgtcg tgtcaggaac ccccagattt
    38281 tccctgactc ttacaccttt ataaacaaac cagatacgta atttccctcc atggttttcc
    38341 acgcctgtcg gatatttcat tttagcttct ctcattggtt agtattgttt ttagtcaagt
    38401 aagatgacgt cttggtcttg ctgatgcctg acgttcaatc cagcgatcaa tttcttccag
    38461 gttgtaaaag catgggctgt tatcccatgg cataccgtca tgggctacat gcttatattc
    38521 ccttccttcc ataaacgatt tttccctggc tttttttagc gttccctttt ttattccttt
    38581 cagcgcaatt agctgttctt cggataccca tttaccggga gagacaatca tgattacttc
    38641 gctcatcgct tttatctctt acttcagatg agcgccggtt gcagaatacc agtcacaacc
    38701 ggcggtggtt gaacattaaa aatcagcctg actcgggatc agtttttgta tcgtagctgt
    38761 aacgtatttt gcctggtgac gggcgtcatc cagtgcattg tggcgcacgc cttcgaatgg
    38821 aataacggtt ctggcatcaa agtcgattgt ttttcccagc tcaacgattg tgcgtacatc
    38881 gcgatcgttg tggtagcgcc acgggcaggg gatgtccaga cgttcgtatg aggtgcgcaa
    38941 aattgcgtta tcgaaagtcg catcattacc ccatacctga acgaattttt catctgagta
    39001 ttcgttgatg aactcccgaa actgcgaaag ggcatccttc agttttacct ggtctgttaa
    39061 aatggcagct ctggcttcac tggactgctt cagccaccat tcgatggttc caccgtcagg
    39121 aacagcccct gtattcattg cgtcagtcag actgataacg atataaaata ctggcccgat
    39181 ttcccctgtt tgtgggtcga agaaaaccgc accaataacc acgatgggcg cattggtgtt
    39241 ggttcccatt gtttcaaggt cgatcatcaa gtggtaccac actctgctgg tggatgtgat
    39301 aacgtgatga ccgttcaccg caattaaggg atctgccgtc tcgccagttt cattatcgct
    39361 ggcgtggtcc tgagcgctgc cagcattctc cttgtgtgga tgttcagcgc cttccatttc
    39421 ctccggatca ttttcctgaa cttcaacctg attctcttca ttgaatgttt cctggtatgt
    39481 tgcgtcgccc atcaccgcgc cacaatcagg gcagttgccg ccgccggtct gaccgcaggc
    39541 ggtgcagatc ttttccggtt cctgttgcac tactggttca ggttgtttcg tttctggctc
    39601 attttgtaac gcatttgggc tgttttgttc cgctttctgg ccgttctgtt ccgtttcttg
    39661 ctggttttgg ttcacagaat cgcgggtttc catccccttc acccatttcg gatcattcgg
    39721 atcgctaatt cctgcaacaa attcaccacg tgatactgca agcagttcat cggcgtcagg
    39781 ctggctgata ttggctgcct gcataatgtt gtttacttcg tcagcggtaa cttttaccgg
    39841 ccctggttgt gcggtcgtgt cagatgcacc agtattttgt tgtgaacctg agtatgtact
    39901 gtttttgcgg acgaaatatt cttctttcgt gatttcagta gccccggcag ccagtgcctt
    39961 atccagacca gaaagtttgt ttgcgcgacc gtatttttcg ccatccttgt cggtgaagag
    40021 gaagtagaac ggcccctcac gctctacaga tggttcgact tccactttgc attcggtttt
    40081 ttcgttgtcc ggaattgccg tttccactgc atcagtttct ggtactggcg acgagagagt
    40141 atcagttgcg atctgatttg ttccttcatc ttcaaacacg ccctttgtag tcaggtattc
    40201 agtaatgtat ttgttcagtg ccacagggtc tttgtgaatg tcgatcggac gctcacggac
    40261 aaggccaaaa atagtctggc ggtcgtagcg aagggcatca ggctgtttgc gcattgatgc
    40321 tgagatacgc ttccagtctt cgcggtcgtt gttgataact tcattttttg cccagcgatg
    40381 gatgctgccg tcaatgtttc cggcatcaat gtcaccaggc cacagagcgt aggccagttc
    40441 atcatccagt gttttccatg tctgcttata ttcgcgacga atgactgcag tgacagggga
    40501 gatattttct gctgagtttt cagtgtgctg tcggttgact ctggcgcggg cgagatcaac
    40561 agcagacgtg tattttccgg tttccttgcg ttcaccttcg cgacgttttt tccagatgcg
    40621 catctctgcc tgaatttcgg gccatttagc tccaggctta catttatgct taacccaccc
    40681 gatggcatgc aacttaagtt ctggatacat ggcgttaact tctggcattt tcatcaacgc
    40741 ttcaacgata tgtccgtcga atgttgccat gtcttcctgc aacagttcct gtgcactaat
    40801 caccatatca acggtgatgt tttcacatgt gtcgaactta accatgacag cgttctgtac
    40861 ttcaggggcc agcttgtcaa aagtggcgtt catcggatct gattcagtct caaccgggac
    40921 aaaggaagct gactcctcat cccagcggtt ttcctgcata tattcagcat cccaggaatc
    40981 gagggcaggg cggggtatac cgggtttatc ctcgcaaaca agaaatttat aagcgcagtc
    41041 ctgagcagcc ggataatgtt ccaggaattg ccagtgaaat tttgcgcggg cgcgacgttc
    41101 atcaccggct tcaatggcag tggctacagc gactgcacct tcttccttta ttgcctgttc
    41161 gtccggaatg gcggcgcaaa taaagacttt actcatgttg ttttaacctc attacagatt
    41221 taagggtgaa caaatctctg ccattgctgg catataaaaa tgaaaccgga tattaattac
    41281 ggtgctgttt taagtcctgc cgggatttcg ttattatcca tgagaataac tttatcgacc
    41341 ggatagcagt taccgggaat tttttgttct gctgcggcag ccatgcattc tttcattgaa
    41401 ccgtaaatgt caataaccat gtcaacaggc tcgccagtat taagaaaaac agtcagagtg
    41461 agggcaatag tagtattcat tgccagtatc ctttttgcat cgggcgtaaa cgtgccagca
    41521 ttgaaagaat gcatatttta tttaatagct cccgttcgtg ttttctctta ttaatggcat
    41581 cttcagtaaa tacagggtta ctgattctga caccaatttc aaaacaacct tcagacgtat
    41641 tgacgtttgg taataacgtt tccattatcg cgtcctcaac aatgaatttt gtgatgcggt
    41701 gcctggtgcc tccaggtgac gttaaccagt taataattaa cgccggatac agagaaccca
    41761 cccataagaa ccaatacgga agtcaactgg cctttttaac tgttccgcgt gcgctgagcc
    41821 gcattcaccg catcacaaaa ttcactttaa aaggggcggg tatcatagga aaaacaaaac
    41881 ggatacccgc caaaaggtaa tcaacatggg ttgttgcagc ggggttgtca ctcaggcgta
    41941 tggtcaacct gacaacccgg tggacatttg cgaggaacgg agaacccccg ccatacttac
    42001 cgccgcgcca tttcgcggag tgccacaacc ggaagcgcac ggtcgacgaa aatttaacga
    42061 caggctatct atgaaccagc aacctcgccg tgcgctttcg cgttatgctc tgacttttca
    42121 gagaaatatc ctttcagtaa actgtcagtg ccggatgctc acccgtgtcc ggcgcacaca
    42181 ctccacctca cccgtggaga actccttaat taccaaccct caggagggtg aatgttaaaa
    42241 tcaacgctta ttgctaaatg cctttatcaa aatcgcatgg taagcagcat ttcaataggc
    42301 gaatctgtag ttaaaagtat tttcgaagag tactttcgaa gagtactttt ctgggcgtga
    42361 ttttaataaa tggaatacca aattaccgcc agcagtttca acgcgtattc tgaaagcaac
    42421 tgaaagagca agtacaattc gcgttaacta tttcattaaa gatttgtggg atctttgata
    42481 tccacagagc ctaaagtatg tgcatatgga tgtgctattg tgcgcccctg taaatttgca
    42541 tcgttttcta aatcttttga tttcaacaga acatcagcaa gactctgaat catgcagaga
    42601 taatcaaaaa cagttgccgt ttctgttctg attgctgaaa taacatgacc attcaggcaa
    42661 acagaaatta cccgttcatc aatatcgttt ttctgttttt gattatcaga accatatagc
    42721 cctgaaaaag cattgcgcac attacgaacc atattatcga tggtttcttt tttggtgtat
    42781 gccgggtcaa ttttcaccag actatcaccg agtgtcgttg cagcaattat ctggatttct
    42841 tttggtaaat ctttaaattc cattattagc ctcgttggtt aaccattaac gtgggtatgt
    42901 aatcattctg gcaatgctta atgccgctgc tttttccaga ttggtgatat cctgctccag
    42961 agcggacaga ttttcagcct gcttagccct ggcttcattg gcccatttca ggtcctgcgc
    43021 agccttaatt ttctggtgca tccactcata aagttcatca tcggtatagt ctggcgcgat
    43081 gatgacgggt tctcgtttct gcatactgat tcctcgcggt gctgcttcgc ttatcagccg
    43141 ttagattttg ccgggctgga aagcgcctgt ttaaattcgc tgaagctgag ggcttcttcg
    43201 ccttcggcaa ggccttcgaa gtattcttcg taagcctttt ccatgattgt gtcgaaatcc
    43261 atatcaccca cctgaatctc tttccagcca gcgacgcgct ccagattcgg ttttaaacgt
    43321 tttgcttttg gtatacgtca ttgcggtgaa cgtgccgtcc tggtttggaa acacgccgta
    43381 caccagagat tcgttgttgc caagatcgat agtatccatg ctgacctcat ttccccttaa
    43441 cgccggggta gcggaactgt ttgctgagaa caccgtgcgg tgtcttgatg caagcaagat
    43501 tagccatgac taacaatttg gtcaagcatt tttgtttgtc atgactaaca ttttgggcaa
    43561 ccaaaaagat aacgcattga ttgcgttatc ttttgtttgt tcgttgacgg gcttttaata
    43621 actcttcaaa gagtttgttg aaatttttta ctcgggcgcg catctcggtg agctgagcat
    43681 cctgttctga ttcaggcaat gcattaaaaa gctcaaggag ctcatgttct ttgggggata
    43741 gagcaactgg ctcctcaata ggtggtgatg gctgcttgtc ttcatcgcca aatagaatcc
    43801 atgttggcga gcactgcagt actttgctga gggcaaaaag attctttcct gtaggttcgc
    43861 tatcatcccg ctcccattgt gaaaccgata cgtgagaaat tttcagggct ttagcaagag
    43921 acctttgggt gtatttgagg ttttttcggc gatatctaat gcgttcgcca atggttaaat
    43981 tttttgtatc catagttagc taatgctaaa tcttattgac tatgtttttg ttaacatcta
    44041 ttttgttagt tatggctaac aattaaggtg ctttaaatgc ttaaaactga cgcacttttg
    44101 tatttcggtt caaaaacaaa acttgcacaa gctgctggta ttcgtttggc ttcgctttat
    44161 agctggaaag gggagctagt acctgaaggt cgcgcgatgc gcctgcaaga ggcatccggc
    44221 ggggaacttc agtacgaccc caaagtttat gacgaatatc gtaaggcaaa gcgggcgggg
    44281 cggttgaaca atgaaaatca cccctgaaca ggtttgtgag gctctggatg cctgggtgtg
    44341 ccgaccagga atgacacagg agcaggcgac gatattaatc acggaagcat tctgggctct
    44401 gaaagaacgc ccgaacatcg atgttcaacg cgtcacgttt aatgatggcg aggttgatca
    44461 acgggcgctg ggcgttaacc gggtgaagat attcgaacgc tggaaagcta tcgacaccag
    44521 ggataagcgg aaaaaattca cggcgctgat tccggcaatt atggaggcta tccggattaa
    44581 tgatttcagg ttgtatcgtg aaattagtga cggaaaaagc atcacgtaca tgatcgccgg
    44641 gttaaacaaa gaatatggcg atgtggtgga gtccgggctg ctttttgcag atccagctgt
    44701 agtggatcgt gaaactgacg aacttataga aaaagccatt gctttcaaac ttgcgtatcg
    44761 acagcaatac caacaaaaag ctggatggaa ttatgagtct tctttttgct gaacgcccac
    44821 tggttataaa cacgcagctg gcaatgaaaa ttggcttaaa cgaagccatt gttttgcaac
    44881 aactgcacta ctggttgaga gataccaact ccggcatgga atgtgatggt gttcgctgga
    44941 tttataacac aacggaacaa tggctggaac agttcccgtt ctggtcagag tcaacgttaa
    45001 agcgcgcgtt tgcaagtctg aaaacgctgg ggcttatgcg ttgtgaaaag ctcaataaat
    45061 caaagcgcga tatgaccaat ttctacacga tcaactacgg gagcgagctt ttagatggtg
    45121 gcaaagtgag cgaatccatc ggttcaaaat gcgccgctcc atcaggtcaa aatgacacga
    45181 tggaagaggt caaaatgaaa cgctccattg gttcaaaacg gctcaatgtc atcgggtcaa
    45241 aatggcctga tgatcttaca gagaatacaa cagagattac tacagagaat aaaaacactt
    45301 ctcgtccgga agcttcgcaa ccggacccgc agacggttga acaggatttt ttaacccgac
    45361 accctgacgc ggttgtgttc agtgcaaaaa aacgccagtg gggcaaccag gaagatttgg
    45421 cgtgtgcgca gtggatctgg ggacgaatcg tgagtcttta cgagcaggcc gccagcgatg
    45481 atggcgagat ttcgcgaccg aaagaaccca actggaccgc atgggccaac gacgtgcgca
    45541 caatgcggat gctggatggc agaactcaca gacaaatttg tgaaatgttt ggtcgggcgc
    45601 agcgggatcc attctgggta aaaaatatca tgagtccgtc aaagcttcgc ggaaaatggg
    45661 atgaactggt tatccgcctg gggcgttcgt ctgtacagcg ttgtgtgaat catatttctg
    45721 agccggatac cgaaattccg ccggggttca gggggtaacg gccatgaaaa atatcgcggc
    45781 aggtggtgtt cttgagcgta tccgtaagct gaccccgcag catgtaatcg cgccgtaccg
    45841 gacagtggat gagtggcgcg agtggcaact ggctgaaggg cgaaaacgta gcgaggagat
    45901 caaccgccag aatcgccagt tgcgggtgga aaaaatcctg aatcgttcgg gcatccagcc
    45961 tctgcacagc aaatgctcgt ttgcgaatta tcaggtgcag aacgacgggc aaaaacatgc
    46021 gctgagccag gcaaaatcca tcgctgacga actgatgacc gggtgcacga attttgtgtt
    46081 cagcggtaag ccgggtaccg gaaagaacca ccttgcagcc gccattggca atcatcttct
    46141 ggcgaaaggt cgcagcgtga ttgtgataac ggtggctgat gtgatgctgg cgttacacaa
    46201 cagctacgac aacaaaaact caggcgaaaa atttttacag gggttgtgtg atgttgacct
    46261 gcttgtcctg gatgaaatcg gaatgcagcg ggatacgcgc aacgagcagg tcacactgaa
    46321 ccagatagtc gaccgcagaa cggcttcgat tcgtagtgtc ggaatgctga cgaacctgaa
    46381 ccacgtagcg atgagtacgc ttcttggcga gcgtgtgatg gaccgcatgg tcatgaacgg
    46441 tggtcgctgg gtgaatttta actgggagag ctggcgttcg aatgtcagac acctgagggt
    46501 tgtgaagtaa tttcaggagg acttatggta aaagttttta ctcccgaaca acgggaagaa
    46561 gtaaaggcgc gtattgtgga actggtacgc agagatggta ggaaaacgcg taaacaactg
    46621 gaaaatgaaa caggggcgac gagacatctg attgaagttc tggcgaaaga actggtagac
    46681 agtggtgttg tatatggttc agggcatgga atattccctt ctgagcaggt acgtaaagac
    46741 tggataaaag cccataaaga gatgtctaaa ggtgcagcga aaaagaagag cgaccctggc
    46801 ctgatttatt cattaccaga tggagagata cgccactacg acaggcgtca gaacataatc
    46861 tgtctcgagt gccggaaaag caaggttatg cagcgtgtgc tggcgtttta tcagggtaat
    46921 tttcaggagg tgatggcgtg agggtgaggg tttatatcgc cggtccaatg acggggtatg
    46981 aaaatttcaa ccgtgaggcg tttcacaagg cagaagagga actgaaacgg gaagggcata
    47041 ccgttttaaa tccggctgtg cttccggatg ggctgacaca gccgcattac atggatattt
    47101 gcatggcaat gattcgttgt gtggatgcga tttacatgct gaaaggctgg cagcggtcag
    47161 caggcgctaa ggcagaactg gcgctggcgg agaaactggg gcatgcggtg atttttcagg
    47221 agacagccag tgtgcaagac taattaccag gcattacgtg aacgttattc accaattcag
    47281 gtgccggaat gttctgtttg tggcgatgaa atgtcgatac agcgcatatt ttccagagcg
    47341 catattgtgt atgcctgcac aggtgagggg gatgatggat attttaaaac tggtcgaact
    47401 tttgcggatg aacattacct gaaatcgcgc gtaaccgtcg ttgatgttag cgatcctgac
    47461 gtactggctt tactgaaaga gctggaagtt aaagacaaac gcattgcaga attgacagac
    47521 gcgcttacac aaatgattaa tgcgcacaaa accacaattc gttttggtca tgaacgcata
    47581 actgaatgtg gtggtgattg cgactcgccg gaaaagatga tttcagaaaa tccggatatc
    47641 agaatggcag aggctgtttt gagagcagga ataaaaactg aataattaaa tttagcacag
    47701 caaataaaat ttaatcctta accggaggga tttctgcacc ctcagaacat caggaggccg
    47761 cctgaaaggg cggtaatgaa aatgactgaa ttaacaaaag aacaattaat cgaagaagcc
    47821 aaattaaaaa tagcgattac gaaatgccac cccaattcag ggatggcgcg agtagagggc
    47881 gagttattca aaattgcact ggcatcgctg gaagcagagc cgatagcgtg gcgatatcgc
    47941 tacgtgaaaa aaggtgttat ggactctcag ggggagttgt gggttggtga ctggaaatat
    48001 gtaccgaaaa aagaggattg taacgacagg ccgaactatg aaattcaggc cttattcact
    48061 gccccaccag tcccggttac atcagaagaa ctggttaaag ctgtgcactt ttatgaacaa
    48121 ctaaaacgcg aaaatccacc agcatccggc aaccagatta atgggttaac tatgccggtt
    48181 aaacgaccag ccgactgaaa aacggaaacc tgattacaga ttcccagata aggcaatgag
    48241 ctacctggcg tagagtgggc tgataagtat ggggaatgtt ttacgatgaa tacttagact
    48301 aaagagtttg taacgctatg taagtgattt tttctggttt agatatttat atgtccggcc
    48361 aaattgaggt gtgtttaaat gtaattgcac attgattgta gggggaataa tgaaaaacgc
    48421 attgcagttt ttgtttgttg cgttctggtt gttcgcatca tgtatgccca tcatcttcac
    48481 agcaaggtat atggaaaaaa ttgatgtttt gatattaatg tttggacata taaatgccct
    48541 ttttttaggg gtgttcatgg cggtcacgtg cattgaatac tggcggtaaa tacagcgaac
    48601 gctattggtt tagttggata tttactgtgc cggacaaaaa cggtttgcag ggaaatctta
    48661 gttaagtaga atgactgtgg gtgcttgagg ctatctgtct caggcatgaa caccaaaagg
    48721 cagatagaga aaagccccag ttaacattac gcgtcctgca agacgcttaa cattaatctg
    48781 aggctcaatc catgctgaac acatgtaggt tagcctctta cgtgccgaaa ggcaaggaga
    48841 agcaggctat gaagcagcaa aaggcgatgt taatcgccct tatcgtcatc tgtttaaccg
    48901 tcatagtgac ggcactggta acgaggaaag acctctgcga ggtacgaatc cgaaccggcc
    48961 agacggaggt cgctgtcttc acagcttacg aacctgagga gtaagagacc cagcggggga
    49021 gaaatccctc gccgcctctg atgtgtcagg catcctcaac gcacccgcac ttaacccgct
    49081 tcggcgggtt tttcgtcgca ttatgaggtt gtaattttag ctaccatgag actatcctaa
    49141 ggatctcaaa cagatctatt ttgtatcaaa tttggtgcat ggctttgcca ataatcggaa
    49201 aacaaaagga ctcattagta tgagctgccc actcaaaaaa cacacgcggt tgagtattcc
    49261 gcctcgcgat aaaagcgttg tggcagtccc tcgcccagtg attgatgaaa actgcgcaca
    49321 tcgtgaacaa gtgaaaaatg cttttgattt cggtttttct cgttatgaga aagccatgga
    49381 agaactttca aaagtgtaat gatgggtatt gtactctatg gctgagattg ttgaaggagt
    49441 gcattacctt acggttgatg atcttgttga aatcaatcat tccctaattg aattacagac
    49501 gccagatgag cccgttggtg ttctgagtcc agataactta agttcttctc aggcccgtcc
    49561 cagcatggtt cgatggtatg aacagactaa tgacatgttt gtactggcat cggtattgat
    49621 tgaaagtctg attcagaatc atccgtttgc taatgcgaac aaacgaacag ctatgatggc
    49681 tggttacgtc ttcttgttgt tgaatggcta tgagttaaca gcaccaggcg atgaaatcgt
    49741 ggaaatggca gagggactgg cctgcaaaac ctatactcga gaagatctcg agaactggtt
    49801 gtgttattgg tctcgcgcgt atgacagcca ggaattatgt aaaacaagcg caactattgt
    49861 tttgtatgaa actatcaagc ttaaaataga acagcaaaac taaaggtgct tccaatgaaa
    49921 acccgcttcg gcgggttttt tattttaatg gactaaattt tagccaattc ttctatttaa
    49981 ccgaccaggg tttgttgatt agctggtatg cctgactaga atcgatgcac ttaagtagcg
    50041 cgcagggaga agagggatgg accccaaaca ggggagtgct atttatctgg aaggattctg
    50101 ttgatgaaaa tcaaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
    50161 gttgagaatg gcgcaattgt cagccactgc cgtattaaat gtttacagtc tcaacaaagg
    50221 aagagtggag ctgcgttaat tcatttggtg gatgggcttg tgacggatgg ttttattttg
    50281 cgtgcaaatg aatttgtcac atcgttgccg tctctgaaag aagctgggat taaggctggt
    50341 ttttctgctt ttgaagatga gtgaattcat ctacaattca gcgcagggct gaacccctgt
    50401 tgagtaacac tgtgccaccg gagaaagccg atggcgcaaa attccagact acacaattct
    50461 gataattcag ccgcctttgc cagcaggcac gggcggcgct ctcatgcatt caaatatgac
    50521 tggtatcagc acgatccctg caccgaagaa caggctgaat ggctgattca gcgctaccgc
    50581 agacacggat acgagattaa gaaagccctc agcctcgatt atcgtctctg gataatctac
    50641 gtcagactcc cttattccga acgcccaccg cgtccgtccc gcacattcca gcaacgcatc
    50701 tggaggtaac gtgcgggtat tacttcgacc tgttccggta ccggaacttg ggctggtggt
    50761 gctaaaaccg ggccgtgaat ccatgcaggt atttcataac cctcgagttc tggtggagcc
    50821 ggaaccgaaa agcatgcgcg gtctgccgtc cggagtcgtt cctgccgttc gccagccatt
    50881 ggcggagtat aaatcactac tgccattttt cagcgatgag cgggtgattc gtgctgctgg
    50941 cggtgctggt gcgctgtctg actggctgtt acgccacgtt aaatcctgcc agtggccaca
    51001 cggcgattat catcacagcg aaaccgtcat acatcgttat ggtatcggcg caatggtgtt
    51061 gtgctggcac tgcgacaacc agctgcgtga ccagacctcc gaatcactcg ggcaacttgc
    51121 tcatcaaaac ctgtcagcat ggatgattga cgtcatacgt cacgcaatca gcggtacgca
    51181 ggagagggag ttatcgctgg ccgaattatc ctggtgggcg gtctgcaatc aggtggctga
    51241 tgcgcttccg gagtctgtat tgcgtcgttc actgggatta ccggtgaaaa aaatccgctc
    51301 cgtatacagt gagagcgaca tcgtaccggg agaacagaca gccaccagca tactgaagca
    51361 gcgcacaaaa aatattgcgc taccgcctca cgtccaccag caccagcaac agaaccc
//
LOCUS       DAZGJT010000025        50061 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZGJT010000025 DAZGJT010000000
VERSION     DAZGJT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50061
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.025"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(144..3293)
                     /gene="acrB"
                     /locus_tag="ACHKFK_001591"
     CDS             complement(144..3293)
                     /gene="acrB"
                     /locus_tag="ACHKFK_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO9051957.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTNYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3316..4509)
                     /gene="acrA"
                     /locus_tag="ACHKFK_001592"
     CDS             complement(3316..4509)
                     /gene="acrA"
                     /locus_tag="ACHKFK_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO9051958.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     AAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4651..5298
                     /gene="acrR"
                     /locus_tag="ACHKFK_001593"
     CDS             4651..5298
                     /gene="acrR"
                     /locus_tag="ACHKFK_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO9051959.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLRKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5426..8788
                     /gene="mscK"
                     /locus_tag="ACHKFK_001594"
     CDS             5426..8788
                     /gene="mscK"
                     /locus_tag="ACHKFK_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO9051960.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRVKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQVQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VSKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQTLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHRRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNATEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            8849..8947
                     /locus_tag="ACHKFK_001595"
                     /pseudo
     CDS             8849..8947
                     /locus_tag="ACHKFK_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9000..9161)
                     /gene="rsmS"
                     /locus_tag="ACHKFK_001596"
     CDS             complement(9000..9161)
                     /gene="rsmS"
                     /locus_tag="ACHKFK_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO9051961.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9175..9702)
                     /gene="priC"
                     /locus_tag="ACHKFK_001597"
     CDS             complement(9175..9702)
                     /gene="priC"
                     /locus_tag="ACHKFK_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO9051962.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLATQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARATDLVEQQTLHREVEAYEARLARCR
                     HALDKIENRLARLTR"
     gene            9772..10149
                     /gene="ybaN"
                     /locus_tag="ACHKFK_001598"
     CDS             9772..10149
                     /gene="ybaN"
                     /locus_tag="ACHKFK_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO9051963.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGAKPRAILLILITFTISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10302..10853
                     /gene="apt"
                     /locus_tag="ACHKFK_001599"
     CDS             10302..10853
                     /gene="apt"
                     /locus_tag="ACHKFK_001599"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO9051964.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10982..12913
                     /gene="dnaX"
                     /locus_tag="ACHKFK_001600"
     CDS             10982..12913
                     /gene="dnaX"
                     /locus_tag="ACHKFK_001600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO9051965.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIKLRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAQTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLLRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12246..12310
                     /gene="dnaX"
                     /locus_tag="ACHKFK_001600"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12966..13295
                     /gene="ybaB"
                     /locus_tag="ACHKFK_001601"
     CDS             12966..13295
                     /gene="ybaB"
                     /locus_tag="ACHKFK_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO9051966.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13295..13900
                     /gene="recR"
                     /locus_tag="ACHKFK_001602"
     CDS             13295..13900
                     /gene="recR"
                     /locus_tag="ACHKFK_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO9051967.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRKENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14010..15884
                     /gene="htpG"
                     /locus_tag="ACHKFK_001603"
     CDS             14010..15884
                     /gene="htpG"
                     /locus_tag="ACHKFK_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO9051968.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTCDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16065..16709
                     /gene="adk"
                     /locus_tag="ACHKFK_001604"
     CDS             16065..16709
                     /gene="adk"
                     /locus_tag="ACHKFK_001604"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO9051969.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRAALEKILG"
     gene            16841..17803
                     /gene="hemH"
                     /locus_tag="ACHKFK_001605"
     CDS             16841..17803
                     /gene="hemH"
                     /locus_tag="ACHKFK_001605"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO9051970.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQYSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALEMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17778..18746)
                     /gene="aes"
                     /locus_tag="ACHKFK_001606"
     CDS             complement(17778..18746)
                     /gene="aes"
                     /locus_tag="ACHKFK_001606"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO9051971.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYY
                     TLERRFWNVGAPEMATRAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYSGMLHAFLHYSRMMKTADDALRDGARFFSDRLQQD"
     gene            18898..20202
                     /gene="gsk"
                     /locus_tag="ACHKFK_001607"
     CDS             18898..20202
                     /gene="gsk"
                     /locus_tag="ACHKFK_001607"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO9051972.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRIDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20258..21934)
                     /gene="ybaL"
                     /locus_tag="ACHKFK_001608"
     CDS             complement(20258..21934)
                     /gene="ybaL"
                     /locus_tag="ACHKFK_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO9051973.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22172..23392)
                     /gene="fsr"
                     /locus_tag="ACHKFK_001609"
     CDS             complement(22172..23392)
                     /gene="fsr"
                     /locus_tag="ACHKFK_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO9051974.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23610..24961
                     /locus_tag="ACHKFK_001610"
                     /pseudo
     CDS             23610..24961
                     /locus_tag="ACHKFK_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
     gene            complement(24998..25477)
                     /gene="ybaK"
                     /locus_tag="ACHKFK_001611"
     CDS             complement(24998..25477)
                     /gene="ybaK"
                     /locus_tag="ACHKFK_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO9051975.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAASDLAKILDAKFADIARRD"
     gene            complement(25681..26475)
                     /gene="ybaP"
                     /locus_tag="ACHKFK_001612"
     CDS             complement(25681..26475)
                     /gene="ybaP"
                     /locus_tag="ACHKFK_001612"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO9051976.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDISLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACETLEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDNGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNEITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            26608..26909
                     /locus_tag="ACHKFK_001613"
                     /pseudo
     CDS             26608..26909
                     /locus_tag="ACHKFK_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057540.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            26945..27286
                     /gene="ybaQ"
                     /locus_tag="ACHKFK_001614"
     CDS             26945..27286
                     /gene="ybaQ"
                     /locus_tag="ACHKFK_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9051977.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27344..29848)
                     /gene="copA"
                     /locus_tag="ACHKFK_001615"
     CDS             complement(27344..29848)
                     /gene="copA"
                     /locus_tag="ACHKFK_001615"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO9051978.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALVVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADIDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVNTKAIEADISA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30110..31042
                     /gene="glsA"
                     /locus_tag="ACHKFK_001616"
     CDS             30110..31042
                     /gene="glsA"
                     /locus_tag="ACHKFK_001616"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO9051979.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTSDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKVGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENTEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTIELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGSSVRGQKMVASVAKQLGYNVFKG"
     gene            31045..32337
                     /gene="ybaT"
                     /locus_tag="ACHKFK_001617"
     CDS             31045..32337
                     /gene="ybaT"
                     /locus_tag="ACHKFK_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO9051980.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            32368..32465
                     /locus_tag="ACHKFK_001618"
                     /pseudo
     CDS             32368..32465
                     /locus_tag="ACHKFK_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32462..32869
                     /gene="cueR"
                     /locus_tag="ACHKFK_001619"
     CDS             32462..32869
                     /gene="cueR"
                     /locus_tag="ACHKFK_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO9051981.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIEQHIEELQSMR
                     NQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(32870..34093)
                     /locus_tag="ACHKFK_001620"
     CDS             complement(32870..34093)
                     /locus_tag="ACHKFK_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9051982.1"
                     /translation="MFSPFRKPEVRLLKNTAAGVIFSVKSGKGFLRQCVIYDPHSHGL
                     IHSLCWQDVPLIRFWSEAGCPTCAEFVYSGFAEDEQGAARFLSALENWNRPWSGITDA
                     FAALTPPFSCMADGYYLLEDRELYPTDGNGHFFWAATDHSSSNPATVAVWDPEYCSFS
                     DTAPCFLLPGQPPSHFNPERATFYRDKPDARALAWYLPDSYLCVLLDGHHKATAAALE
                     GRPLKTLVLSTATHFNDEQQTLVFPGGECLHKTELQYHIPKLTACKTLPPSAWESFGP
                     DKHISPSETWSEELKQSVSRYPSLDQAWQIVEAGNLSETRIKSMIQQGLGEDEKADII
                     LQALFSTHSPLFIDFARFVISHPAYAIYRPLTFRLMAQNRTPQADAFFLDFAINDDGE
                     RPELTKIMDDYFRKP"
     gene            complement(34212..34670)
                     /gene="ybbJ"
                     /locus_tag="ACHKFK_001621"
     CDS             complement(34212..34670)
                     /gene="ybbJ"
                     /locus_tag="ACHKFK_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO9051983.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLLPLGWEWQGVMFAVLTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGTGTHVEVIAIEGITLIIRAVIA"
     gene            complement(34667..35584)
                     /gene="qmcA"
                     /locus_tag="ACHKFK_001622"
     CDS             complement(34667..35584)
                     /gene="qmcA"
                     /locus_tag="ACHKFK_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO9051984.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLHIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            35730..36407
                     /gene="fetA"
                     /locus_tag="ACHKFK_001623"
     CDS             35730..36407
                     /gene="fetA"
                     /locus_tag="ACHKFK_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO9051985.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLCAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            36394..37173
                     /gene="fetB"
                     /locus_tag="ACHKFK_001624"
     CDS             36394..37173
                     /gene="fetB"
                     /locus_tag="ACHKFK_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO9051986.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(37236..38090)
                     /gene="cnoX"
                     /locus_tag="ACHKFK_001625"
     CDS             complement(37236..38090)
                     /gene="cnoX"
                     /locus_tag="ACHKFK_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO9051987.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGLFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(38151..38960)
                     /gene="ybbO"
                     /locus_tag="ACHKFK_001626"
     CDS             complement(38151..38960)
                     /gene="ybbO"
                     /locus_tag="ACHKFK_001626"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9051988.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVGKVRHAFISEKPKMRYPVTLVAWAVMVLKRLLPGRVMDKILQG"
     gene            complement(38950..39573)
                     /gene="tesA"
                     /locus_tag="ACHKFK_001627"
     CDS             complement(38950..39573)
                     /gene="tesA"
                     /locus_tag="ACHKFK_001627"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO9051989.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            39544..40230
                     /gene="ybbA"
                     /locus_tag="ACHKFK_001628"
     CDS             39544..40230
                     /gene="ybbA"
                     /locus_tag="ACHKFK_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO9051990.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            40227..42641
                     /gene="ybbP"
                     /locus_tag="ACHKFK_001629"
     CDS             40227..42641
                     /gene="ybbP"
                     /locus_tag="ACHKFK_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO9051991.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLMAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAITGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLAFLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVTPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNGDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQSKVFDFPWEPDWRLWIVLPCSGALLLSLCGGWLGARLLKGKAL
                     FRQFAG"
     gene            complement(42882..43976)
                     /gene="mnmH"
                     /locus_tag="ACHKFK_001630"
     CDS             complement(42882..43976)
                     /gene="mnmH"
                     /locus_tag="ACHKFK_001630"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO9051992.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGICYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPHGILCCARGGQ
                     RSHIVQRWLHDAGIDYPLVEGGYKALRQTAIQATIELSQKPIVLIGGCTGCGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDES
                     RMIGSNHLPECLRERMTQATIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVVEWVKAQ"
     gene            complement(44045..44971)
                     /gene="allS"
                     /locus_tag="ACHKFK_001631"
     CDS             complement(44045..44971)
                     /gene="allS"
                     /locus_tag="ACHKFK_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO9051993.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTATGEHLLCQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVARLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMLDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            45201..45683
                     /gene="allA"
                     /locus_tag="ACHKFK_001632"
     CDS             45201..45683
                     /gene="allA"
                     /locus_tag="ACHKFK_001632"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO9051994.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQKRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            45761..46576
                     /gene="allR"
                     /locus_tag="ACHKFK_001633"
     CDS             45761..46576
                     /gene="allR"
                     /locus_tag="ACHKFK_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000141283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO9051995.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHL"
     gene            46666..48447
                     /gene="gcl"
                     /locus_tag="ACHKFK_001634"
     CDS             46666..48447
                     /gene="gcl"
                     /locus_tag="ACHKFK_001634"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO9051996.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKILMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            48460..49236
                     /gene="hyi"
                     /locus_tag="ACHKFK_001635"
     CDS             48460..49236
                     /gene="hyi"
                     /locus_tag="ACHKFK_001635"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO9051997.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPTGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            49336..>50061
                     /gene="glxR"
                     /locus_tag="ACHKFK_001636"
     CDS             49336..>50061
                     /gene="glxR"
                     /locus_tag="ACHKFK_001636"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9051998.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKLLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLA"
BASE COUNT        12008 a        12969 c        13314 g        11770 t
ORIGIN      
        1 aatcagtatt ttttatgaat tttatgcggg gttaatactg gttttcgtat gagatcctga
       61 gttggtggtt caatacatcc ttaatgttcg taggttatgc ataaaaaagg ccgcttgcgc
      121 ggccttagtg attacacgtt gtatcaatga tgatcgacag tatggctgtg ctcgatatct
      181 tcattcttgc ggctaaagcg gcggcgaacc accacaaaga ataccggaac gaagaagatt
      241 gccagtaccg ttgcggtcac catcccgccc attacaccgg tacctactgc gttctgcgcg
      301 ccggaaccag caccagtact gataaccagc ggcataacgc cgaggataaa cgccaacgag
      361 gtcatcagga ttgggcgtaa acgcatccgc accgcatcaa gcgtcgcttc aatcagacct
      421 ttaccttctt tatccatcaa gtctttggcg aattcgacga taagtatcgc gttcttcgcc
      481 gacaaaccaa tggttgtgag caggcctacc tggaagtaaa cgtcattggt caggccacgg
      541 aaggtggcag ccagcaacgc accgataacc cccagcggaa cgactagcat aacggagaac
      601 ggaatcgacc agctctcgta cagcgcagcc agacacagga acacgacaat caacgaaatc
      661 gcgtacagtg aaggtgcctg gttgccggag agacgttcct gataggacat tcccgtccag
      721 tcatagccaa caccggtagg cagtttgctc gccagttgtt ccatcagctc cattgcttca
      781 ccggtacttt tacccggtgc cgcctggcct aagatttcca tagatggcag gccgttgtaa
      841 cgttccagac gcggcgaacc gtactcccaa cgagaagagg agaacgccga gaacggcacc
      901 atctgaccat cagcagcacg aacataccag tcgccgatat catccggcag catacggtat
      961 ttcgcttctg acatgacgta aactttcttc acacgaccgc ggtcgataaa gtcgttcaca
     1021 tagctaccgc cccatgcagc gcccagagtg gtgttaatgt cgttgataga aacacccagc
     1081 gcctgcgctt tttcctggtc gatatcaatc ttaaactgcg gggtatcttc cagaccgttt
     1141 ggacgtacgc tggtcaacat atcagggtgc ttcgctgctt ctgcaagcaa ctggttacgc
     1201 gcctgagtca gtttttcgtg accaaggcca gcctggtcaa tcagctcaaa gtcaaagccg
     1261 gttgcggtac ccagttccac gattgcgggc aggttaaagg cgaaaaccat cgcatctttg
     1321 atttgcgaga aagcgcgtgt tgcacgcatg gtaatcgctt caactttgtt ttcttcgccc
     1381 ggacgatcgg cccaatcctt caaggaaacg aacgcaatac cggtattctg accacgtccc
     1441 gcaaagccga agccgttaac ggcgaacacc gactcaacgt tgttcttttc tttggtcaga
     1501 tagtaattcg ttacctcatt gagcactttc tgcgtacgtt cctgcgttgc acctgctggc
     1561 agctgaacca tggtcataaa tacgccctgg tcctcatctg gcaagaagga gcttggcaga
     1621 cgcacgaaca gataggccat accgaccacg atgatcagat acagcaccag gtaacgcccc
     1681 gtactgcgca gaataccgcc tacgctgtcg gtgtagtggt gcgtgctctt ctcgaacatg
     1741 cggttaaacc agccgaagaa gcctttttta ccttccccgt gatcgccttt ggcaatcggt
     1801 ttcagcatgg tggcacaaag agccggagtc aggatcaacg ccaccagtac cgacagcgcc
     1861 attgctgaaa caatggtaat agagaactga cgatagatag caccagtaga accgccaaag
     1921 aaggccatcg gtacgaatac cgccgacagt accatcgcga taccgaccag agcgccctga
     1981 atctgcccca tcgacttacg ggtggcttct tttggcggca aaccttcttc cgccatcaca
     2041 cgctcaacgt tttctaccac aacgatagcg tcatccacca acaggccgat ggcgagcacc
     2101 atcccgaaca ttgttagcgt gtttatcgag aagccaaagg cggcaaggac ggcaaaggtc
     2161 ccgagcaata ccaccggtac ggcaatggtc ggaatcaacg tcgcgcggaa gttctgcaag
     2221 aacagataca ttaccaggaa cacgaggatg atcgcttcga ccagcgtttt aaccacttcg
     2281 tgaatagaga ttttcacgaa cggtgtggtg tcatacgggt aaacaatttt cagacccgac
     2341 gggaagaacg gttccatctt cgccagttca gcacggattg ccgcagcggt atccagcgcg
     2401 ttcgcaccgg tcgccagctt gatccccaga ccggaagccg gttggccgtt aaactctgcg
     2461 atgatgtcgt agttctcacc acccagctca attttcgcca catcacgcag cagcacgcgg
     2521 gaaccatcct gattcacttt cagcaggatt ttgccgaact cttcagtaga ggtcagacgc
     2581 gtctgagcaa taatagaggc gttaagctgt tggcctttca ccggcggcgt accaccgagc
     2641 tgaccagctg caacctgggc gttctgtgct ttgatggcgg taatgacatc aaccggcgtt
     2701 agctggaatt tgttcagttc attcgggttc atccagatac gcatcgcgta ctgtgaaccg
     2761 aacaactgaa cgtcacccac gcctgacgta cggctgatgg catctttcat attcgccgcc
     2821 acgtagtcgg agatatcctc ctgcgtcatg gtgccatcgg tgttgataac gccgacaacc
     2881 atcaggaagc tgctggatga tttctcaacg ctcacccctt gctgctgtac ttcttgtggc
     2941 agcaacggca tcgccagctg cagtttgttc tgcacctgaa cctgcgcgat atccgcatca
     3001 gtaccagact caaaggtcag ggtgatctgc acggtacccg tggagtcact gttagaggac
     3061 atgtacatca ggttatcgat accgttcata ttctgttcga taacctgtgt caccgtgtcc
     3121 tgcactgttt tcgcatcagc gccagggtag gaggcggaga tcgttactgc cggcggtgca
     3181 atcgtaggat attgcgccac cggcagtttg aggatcgcca gcccccctgc caacatgatg
     3241 ataatggcga tcacccacgc aaaaatcggg cgatcgataa agaaattagg catgtcttaa
     3301 cggctcctgt ttaagttaag acttggactg ttcaggctga gcaccgcttg cggcttgctg
     3361 gttattatca gcggtaactt cttgtgcttt tacctggaca ccaggacgca ctttctgcag
     3421 cccacttatt actacgcgat cgcctgcttt cagaccttct gtcaccagcc acttatcgcc
     3481 gatagcctgg cttgcaacga tcggacgggt ttccactttg tcatccgcgc caaccaccag
     3541 tacggtggca tcgccacgcg gcgtacgggt tacgccctgt tgcgggacta aaatagcgtt
     3601 tggattaagc ccttcttcca gacgtgcacg cacgaacata cccggcagca gagtgtgatc
     3661 cgggttcggg aagatagcgc gtagggtgat agacccagtg gtctgatcaa cggtaacgtc
     3721 agagaattcc agcgtaccgt cctgcgggaa cttaatgccg tcactggtga tcagcgacac
     3781 tttggctttg ccgttctctt gtttcagcgt gccattcgcc agttcctgtt tcaggcgcag
     3841 gaagtcgttg ctggactggg tcacatcaac gtagatcgga tcaagttgct gcacggttgc
     3901 cagcgcagtc gcctgaccgt tctgtaccaa tgcgccttcc gtcacgtttg acttaccaat
     3961 gcgaccacta atcggagagg taactttggt gtaagccaga ttgattcgcg cagtttcaac
     4021 ggcagctttc gccgcagtta ccgcagcatt cgcctgttgc gcatcagcca gagcctgatc
     4081 gtactcttgc ttactgatgt actgagtacc gagcaatttc tgataacgat tcaccgtcaa
     4141 ttgcgcgata ttggctgcag cctgggcttt cgccagatca cctttcgcac tgtcgtatgc
     4201 cgcctgatag gtcgcaggat caatctgata gagagagaca cctgcttcga tgtcgctacc
     4261 ttctttgaaa ttacgcttca ggataatccc gctaacttga ggacgaactt ctgcgatccg
     4321 gtaggcactg gtgcgacccg gaagctcggt tgtgatctgc agaggttcag ttttgactgt
     4381 tactacgcca acggcgggca tctgctggcc accttgttgg gcctgtttgt cgtcacatcc
     4441 tgttagggct aagctgcctg agagcatcag aacgaccgcc agaggcgtaa accctctgtt
     4501 tttgttcata tgtaaacctc gagtgtccga tttcaaattg gtcaatggtc aaaagttaat
     4561 aaacccattg ctgcgtttat attatcgtcg tgctatggta catacattca caaatgtatg
     4621 taaatctaac gcctgtaaat tcacgaacat atggcacgaa aaaccaaaca agaagcgcaa
     4681 gaaacgcgcc aacacatcct cgatgtggct ctacgtcttt tctcacagca gggggtatca
     4741 tccacctcgc tgggtgagat tgcaaaagca gctggcgtta cgcgcggtgc aatctactgg
     4801 cattttaaag acaagtcgga tttgttcagt gagatctggg aactgtcaga atccaatatt
     4861 ggtgaactcg agcttgagta tcaggcaaaa ttccctggcg atccactctc agtattaaga
     4921 gagatattaa ttcatgttct tgagtccacg gtgacagaag aacggcgtcg attattgatg
     4981 gagattatat tccacaaatg cgaatttgtc ggagaaatgg ctgttgtgca acaggcacaa
     5041 cgtaatctct gtctggaaag ttatgaccgt atagaacaaa cgttaaaaca ttgtattgaa
     5101 gcgaaaatgt tgcctgcgga tttaatgacg cgtcgcgcag caattattat gcgcggctat
     5161 atttccggcc tgatggaaaa ctggctcttt gccccgcaat cttttgatct tagaaaagaa
     5221 gcccgcgatt acgtcgccat cttactggag atgtatctcc tgtgccccac gcttcgtaac
     5281 cctgccacta acgaataacc ctgaatctga ctccaggaat tttcctggac attttcgtcg
     5341 ttgctattct ggttcactgc gtcgtgatat tcttgcggtt tgactttttc aggtcgttct
     5401 tcaggttcag aaaccttcat tcatcatgac tatgttccag tattacaaac gatcacggca
     5461 ttttgttttt tcagcattta ttgcttttgt ttttgtcttg ttatgccaga acacggcgtt
     5521 tgcacgggcg tcatcgaatg gtgatttacc gacaaaagcg gacctgcagg cacaacttga
     5581 ctcactgaat aaacaaaaag atctttctgc tcaggacaaa ctggtgcagc aggatctgac
     5641 agatacatta gccaccctcg ataaaatcga tcgcgtaaaa gaagagacag ttcagctacg
     5701 gcaaaaagtc gctgaagcgc cggaaaaaat gcgccaggcg acagcggcgt taacagcact
     5761 tagtgatgtc gataacgacg aagaaacgcg caaaattctg agcacgcttt cgttgcgcca
     5821 gctggaaact cgcgttgctc aggcgctgga cgatttgcaa aacgcacaaa atgatctggc
     5881 gtcttataac agccaactgg tttcgctaca gacgcagccc gaacgcgtac aaaatgcgat
     5941 gtataacgct tcgcagcagc tgcaacaaat tcgcagtcgt ctggatggga ctgatgtcgg
     6001 cgagactgcc ttacgtccca gccagaaagt gttaatgcag gtccagcagg ctttgctgaa
     6061 tgccgagatt gaccagcagc gtaaaagcct ggaagggaac actgtcttgc aggatacctt
     6121 gcaaaagcag cgtgattacg tgacggcgaa cagcgcccgt ctggagcacc agttacaact
     6181 gttgcaagaa gcggtaaaca gcaagcgcct gactttaacc gaaaaaacgg cgcaggaagc
     6241 tgtctccccg gatgaagccg cgcgtattca ggctaatccg ctggtgaagc aggaactgga
     6301 aattaaccag cagttaagtc agcgtctgat taccgcgact gaaaacggta atcagttgat
     6361 gcaacaaaac attaaagtca aaaactggct ggagcgtgcg ctgcaatcgg aacgtaatat
     6421 taaggagcag attgccgttc tgaagggcag tctgctgttg tcacgtatcc tgtaccagca
     6481 acaacaaaca ctcccctcgg cagatgaact ggaaaacatg accaaccgca tcgcggattt
     6541 gcgtctcgaa cagtttgaag tcaaccagca gcgtgacgcg ctgttccaga gcgatgcgtt
     6601 cgtcagcaaa ctggaagaag gtcacaccaa tgaagttaac agcgaagttc acgatgcgtt
     6661 attgcaagta gttgatatgc gccgtgaatt gctggatcag cttaacaaac agttgggtaa
     6721 tcagctgatg atggccatta acctgcaaat taaccagcag cagttaatga gcgtatcgaa
     6781 aaacctgaaa tccatcctga cgcagcaaat tttttgggtg aacagtaacc gtccgatgga
     6841 ctgggactgg attaaagcgt tcccgcaaac gctgaaagat gaatttaagt cgatgaaaat
     6901 cacggtgaac tgggaaaaag cctggcccgc cgtattcatc gccttcctcg ctggtttgcc
     6961 gctgctgttg attgctgggc tgattcacag gcgtttaggc tggctgaaag catatcaaca
     7021 aaaactggcc tcggcggtag gttccctgcg taacgatagc cagctcaaca caccaaaagc
     7081 gatccttatc gacctgatcc gtgcgctgcc ggtgtgcctg attattctcg cggttggcct
     7141 gattctgttg accatgcagc tcaacatcag cgaactgcta tggtcgttca gcaaaaaact
     7201 ggcgattttc tggctggtgt ttggcctgtg ctggaaggta ctggagaaaa acggtgttgc
     7261 cgtacgtcac tttggtatgc cggaacagca gaccagccac tggcgtcggc aaattgtccg
     7321 catcagcctc gcgttgctgc cgatccattt ctggtctgtg gtggcagaac tctccccgct
     7381 gcatctgatg gatgatgtgc tggggcaggc gatgattttc ttcaacctgc tgctgattgc
     7441 cttcctggtg tggccgatgt gccgcgaaag ctggcgtgat aaagagtcgc acaccatgcg
     7501 actggtcacc attaccgtgc tgtcgattat cccgattgcg ttgatggtgc tgaccgccac
     7561 gggttacttc tacaccacgc tgcgcctctc cgggcgttgg attgaaaccg tttatttggt
     7621 gatcatctgg aacctgctgt accagacggt attgcgcggc ttaagcgtag cggcgcggcg
     7681 tatcgcctgg cgtcgtgcac tggcgcgtcg gcagaatctg gtgaaagagg gcgcagaagg
     7741 tgccgaaccg ccggaagaac ccaccattgc gctggagcag gttaaccagc agacgctgcg
     7801 tattaccatg ttgctgatgt ttgcgctgtt cggtgtcatg ttctgggcaa tttggtctga
     7861 tttgatcacc gtgttcagct atctcgacag cattacgctc tggcattaca acgctactga
     7921 agctggtgct gcggtggtga aaaacgtcac catgggcagt ctgttgtttg cgattatcgc
     7981 ctcaatggtg gcctgggcgt tgattcgcaa cctgcctggt ttgctggaag tgctggtgct
     8041 ctcgcgactg aatatgcgcc agggcgcgtc gtatgccatt actaccatcc ttaactacat
     8101 cattattgcc gttggtgcga tgacggtgtt cggatcgctg ggcgtctcgt gggataaact
     8161 ccagtggctg gcggcagcat tatccgtagg tcttggtttt ggtttacaag agatcttcgg
     8221 taacttcgtt tccggtttga tcattctgtt cgaacgtccg gtgcgcatag gcgacacggt
     8281 aaccattggt agcttctcgg gtacggtcag caagatccgt attcgtgcga caacgattac
     8341 cgatttcgat cgcaaagaag tgattatccc gaacaaagcg tttgttaccg agcgtctgat
     8401 caactggtcg ttgactgaca cgactacgcg tctggtgatc cgtctcggcg tagcctatgg
     8461 ctccgatctg gaaaaagtgc gtaaagtgtt gctgaaggcg gcgactgagc acccaagggt
     8521 gatgcacgaa ccaatgccgg aagtcttctt tacggcattt ggtgccagca cgttggatca
     8581 tgagctgcgt ctgtatgtac gtgaactgcg tgaccgtagc cgtactgtcg atgagctgaa
     8641 ccgtactatc gatcagttgt gccgtgaaaa cgacatcaac attgccttta accagcttga
     8701 agtgcatctg cacaacgaga agggcgatga ggtgacggaa gtcaaacgcg actacaaagg
     8761 cgacgatccg acaccagcgg ttgggtaata accaaagggg gcgacattta gttgccccga
     8821 gattactaac aaagtgcgcg ttgttcatgc cggatgcggc gtgaccgcct tatccggcct
     8881 acgaaaccgc aagaattcaa tatattgcag gagcggtgta ggcctgataa gcgtagcgca
     8941 tcaggcagtt ttgcgtttgc ccacaatctt aggggacatt tagcggcccc atttatttct
     9001 cacttttccg cctcatcatc gcgcgttaat ttcttttcat aatcgcgttt tacaatatcc
     9061 agcgcgcgca gcacggtgcg ggctgggatc tgattttctt ccagtagcac aatcaaatcg
     9121 acggccagtt tgacatcgtc aggggcattt tccagtgaca tactctctcc attgttagcg
     9181 ggttaaacgc gctaacctgt tttcgatttt atccagcgca tggcggcagc gtgccaggcg
     9241 cgcttcatag gcttccactt cacgatgcag cgtctgctgt tccacgagat cggtcgctcg
     9301 cgccagacgg gctcgacgtt cggcaaccat ctcacgtagc cgccgctcaa aatcctgatg
     9361 ctgaatacgt ttacgctgcc agcgggcaat tttcggtggc gcactgtccc actcgcgcaa
     9421 tgaccaggcg gaggcttcac gcgcgatggc ttccagttgc gtcgccagat gttccgccag
     9481 ccaggccact tgcggcagtt gctgttgctc aactgcatga cgaagcgcag ccagattatc
     9541 gcccgcctca tcgagacaag cctgcagtgt tgtcgcacgg gtctgaaaaa ggtgcctgtc
     9601 aaaacgggca cttagcgtgg caaattgcgc taccggggca caacgctgac gcagcgtggc
     9661 gagctgtcct tccagttttt ccagcagcag ggcggttttc acgataagac ctcaatgaaa
     9721 atgataattg ttatgctaaa gtagccactc tgtaagctga caacactcaa tatgcaacga
     9781 atcattttaa tcatcattgg ctggctggcg gtagtgctgg gtacgctggg cgtggtatta
     9841 ccggtattac cgacgacgcc gtttatcctg ctggcggcct ggtgctttgc ccgttcttcc
     9901 ccgcgctttc acgcctggtt gctgtaccgc tcatggtttg gcagctatct acgtttctgg
     9961 cagaaacatc atgcgatgcc gcgcggcgca aaaccacggg cgattttgct tattttgatt
    10021 acgtttacga tttcgctgtg gttcgtccag atgccgtggg tgcgcatcat gttgctggta
    10081 attctcgcct gtttgctttt ttatatgtgg cgaattccgg tgattgatga aaagcaagaa
    10141 aagcactgaa gcacaacaat cgcagttgca attattgcgt acagccagta cattctggcg
    10201 ttttcgagca caggcgcagg cggtcaaagg ttaaacaact gttacttttg atacgttcaa
    10261 aacgcgccgt gagtaccacc gtaacaagca ggcatacact tatgaccgcg actgcacagc
    10321 agcttgagta tctcaaaaat agcatcaaaa gcattcagga ctacccaaaa cccggcattc
    10381 ttttccgcga tgtcaccagc ttactggaag acccgaaagc ttacgctctc agcatcgact
    10441 tgctggttga gcgttacaaa aatgcgggca ttaccaaagt ggtcggcacc gaagcgcgtg
    10501 gcttcttgtt tggcgctccg gtagctctgg gtctgggcgt tggctttgta ccggtccgta
    10561 aaccgggcaa actgccgcgt gaaaccatca gtgaaactta cgacctggaa tacggcaccg
    10621 atcagctgga aatccacgtt gatgccatca aaccgggcga taaagttctg gtggtggacg
    10681 atctgctggc aaccggcggc actatcgaag cgaccgttaa actgatccgt cgtctgggtg
    10741 gtgaagtagc tgacgctgcg ttcattatca acctgttcga tctcggcggc gaacagcgtc
    10801 tcgaaaaaca gggcattacc agctacagcc ttgtcccgtt cccgggccat taattatcgt
    10861 cagtcttgcg ctgctcacgc tacgggcagc acaagatgtg cattcagcct cgccgttctg
    10921 acggggctgt gttagcatta ccccttcgtg aatccacctt ccagcgtttc agagcctgcc
    10981 aatgagttat caggtcttag cccgaaaatg gcgcccacaa acctttgctg acgtcgtcgg
    11041 ccaggaacat gtgctgaccg cactggcgaa cggcttgtcg ttagggcgta ttcatcatgc
    11101 ttatcttttt tccggcaccc gtggcgtcgg aaaaacctct atcgcccgac tgctggcgaa
    11161 ggggctaaac tgcgaaactg gcattaccgc gacgccgtgc ggcgtgtgcg ataactgtcg
    11221 tgaaatcgag caggggcgct ttgtcgatct gattgaaatc gacgccgcct cgcgcaccaa
    11281 agttgaagac actcgtgacc tgctggataa cgtccagtac gcgccagcgc gtggtcgttt
    11341 taaagtttat ctgatcgacg aagtgcatat gctgtcgcgc cacagcttta acgcactgtt
    11401 aaaaaccctt gaagaaccgc cggagcacgt caagtttctg ctggcgacga ccgatccgca
    11461 gaaattgccg gtgacgattt tgtcacgctg tctgcaattt catctcaaag cgctggatgt
    11521 cgagcaaatt cgccatcagc ttgagcacat cctcaacgaa gaacatatcg ctcacgagcc
    11581 acgggcgctg caattgctgg cacgcgccgc tgaaggcagc ctgcgagatg ccttaagtct
    11641 gaccgaccag gcgattgcca gcggtgacgg ccaggtttca acccaggccg tcagtgcgat
    11701 gctgggtacg cttgacgacg atcaggcgct gtcgctggtt gaagcgatgg tcgaggccaa
    11761 cggcgagcgc gtaatggcgc tgattaatga agccgctgcc cgtggtatcg agtgggaagc
    11821 gttgctggtg gaaatgctcg gtttgttgca tcgtattgcg atggtacaac tttcgcctgc
    11881 tgcacttggc aacgacatgg ccgccatcaa gctgcggatg cgtgaactgg cgcgcaccat
    11941 accgccgacg gatattcagc tttactatca gacgctgttg attggtcgca aagaattacc
    12001 gtatgcgcca gaccgccgta tgggcgttga gatgacgctg ctgcgcgcgc tggcgttcca
    12061 tccgcgtatg cctctgcctg agccagaagt gccacgccag tcctttgcgc ccgttgcgca
    12121 aacggcagta atgacgccaa cacaggtgcc gccgcaaccg caatcagcgc cgcagcaggc
    12181 accgactgta ccgctcccgg aaaccaccag ccaggtgctg gcggcgcgcc agcagttgct
    12241 gcgcgtgcag ggagcaacca aagcaaaaaa gagtgaaccg gcagccgcta cccgcgcgcg
    12301 gccggtgaat aacgctgcgc tggaaagact ggcttcggtc accgatcgcg ttcaggcgcg
    12361 cccggtgcca tcggcgctgg aaaaagcgcc agccaaaaaa gaagcgtatc gctggaaggc
    12421 gaccactcca gtgatgcagc aaaaagaagt ggtcgccacg ccgaaggcgc tgaaaaaagc
    12481 gctggaacat gaaaaaacgc cggaactggc ggcgaagctg gcggcagaag ccattgagcg
    12541 cgacccgtgg gcggctcagg tgagtcaact ttcgctacca aaactggtcg aacaggtggc
    12601 gttaaatgcc tggaaagagg agagcgacaa cgcagtatgt ctgcatttgc gctcctctca
    12661 gcggcatttg aacaaccgcg gcgcacagca aaaactggct gaagcgttga gcacgttaaa
    12721 aggttcaacg gttgaactga ctatcgttga agatgataat cccgcggtgc gtacgccgct
    12781 ggaatggcgt caggcgatat acgaagaaaa acttgcgcag gcgcgcgagt ccattattgc
    12841 ggataataat attcagaccc tgcgtcgatt cttcgatgcg gagctggatg aagaaagtat
    12901 ccgccccatt tgatcgtaag cacagcttac gttcgtcatc cttaacgtga ttgagagaga
    12961 aacctatgtt tggtaaaggc ggtctgggta acctgatgaa gcaagcccag cagatgcaag
    13021 aaaaaatgca gaaaatgcag gaagagatcg cgcagctgga agtcaccggc gaatctggcg
    13081 caggtctggt aaaagtgacc atcaacggtg cacacaactg ccgtcgcgta gagatcgacc
    13141 cgagcctgct ggaagacgac aaagagatgc tggaagacct ggtggctgca gcattcaacg
    13201 acgcagcacg tcgtattgaa gaaacgcaga aagaaaaaat ggcctctgta tcctccggaa
    13261 tgcagctgcc gcctggcttt aagatgccgt tctgatgcaa accagcccgc tgttaacaca
    13321 gcttatggaa gcactgcgct gcctgccggg cgttggcccg aagtcggcgc agcgtatggc
    13381 gttcacgctg cttcagcgcg atcgtagcgg cgggatgcgt ctggcgcagg cgctcacccg
    13441 ggcgatgtcg gaaatcggcc actgcgccga ttgccgcacc ttcaccgaac aggaagtctg
    13501 taacatctgt tcgaatccgc gtcgtaagga aaacggtcaa atctgcgtgg tggagagtcc
    13561 ggcggacatc tacgccattg agcagacggg gcagttttca ggccgttatt ttgtgttgat
    13621 ggggcacctg tcaccgctgg acggcatcgg tccggatgat atcgggcttg atcgtctgga
    13681 acagcgtctg gcagaggaaa aaatcactga agtgatcctc gccaccaacc ccacggttga
    13741 aggtgaagct accgctaact acattgccga gctttgtgcg caatatgacg tggaagccag
    13801 ccgaatcgct catggcgtac cggttggcgg cgaactggaa atggtcgatg gcaccacgct
    13861 gtcacactcc cttgccgggc gtcataagat tcgtttttaa gcaaacgaga gcaggatcac
    13921 ctgctctcgc ttgaaattac tctcccttgt ccccatctct ccctcatcct gtttttaacc
    13981 ttaaaatggc attattgagg tagacctaca tgaaaggaca agaaactcgt ggttttcagt
    14041 cagaagtgaa acagcttctg cacctgatga tccattctct ctattccaat aaagaaatct
    14101 tcctgcgtga gcttatctct aacgcctccg atgcggcgga caagctgcgt ttccgcgcgc
    14161 tttctaaccc ggacctgtac gaaggtgatg gtgaactacg cgttcgtgtc tctttcgata
    14221 aagacaagcg cacgctgacc atctccgata acggcgtggg gatgacctgc gacgaagtga
    14281 ttgaccatct ggggactatc gctaaatccg gcaccaaatc attcctcgaa tccctgggtt
    14341 ctgaccaggc gaaagacagc cagctgatcg gtcagtttgg tgttggtttc tactctgcgt
    14401 ttatcgtggc tgacaaagtg accgtgcgta ctcgcgcggc aggcgaaaaa ccagaaaacg
    14461 gcgtcttctg ggaatcggct ggcgaaggtg aatacaccgt tgccgacatc actaaagaag
    14521 atcgtggtac tgaaatcacc ctgcatctgc gtgaaggcga agacgagttc ctcgatgact
    14581 ggcgtgtgcg ttccatcatc agcaaatatt ccgaccatat cgcgctgccg gtagagatcg
    14641 aaaaacgcga agagaaagac ggcgaaaccg ttatctcctg ggagaaaatc aacaaagcgc
    14701 aggcgctgtg gactcgtaac aagtcggaaa tcaccgatga agagtacaaa gagttctaca
    14761 aacacatcgc ccacgacttt aacgatccgc tgacctggag ccataaccgc gttgaaggta
    14821 agcaggagta caccagcctg ctgtacattc cgtctcaggc accgtgggat atgtggaacc
    14881 gcgatcataa acacggtctg aaactgtatg ttcagcgtgt gttcatcatg gacgacgcag
    14941 aacagttcat gccgaactat ctgcgcttcg tgcgtggtct gattgactcc agcgatctgc
    15001 cgctgaacgt ttcccgtgaa atcctccagg acagcacggt aacgcgtaac ctgcgcaatg
    15061 cgctgaccaa gcgtgtgctg caaatgctgg aaaaactggc gaaagacgac gcggaaaaat
    15121 accagacctt ctggcaacag tttggcctgg tactgaaaga aggtccggcg gaagatttcg
    15181 ctaaccagga agcgatcgcc aaactgctgc gttttgcttc tacccatacc gattcttctg
    15241 cgcagaccgt gtctctggaa gactacgttt cccgcatgaa agaagggcag gagaaaatct
    15301 actatatcac cgcagacagt tacgcggcag cgaagagcag cccgcacctg gaactgctgc
    15361 gtaagaaagg catcgaagtt ctgctgcttt ccgaccgcat cgatgagtgg atgatgaact
    15421 atctgactga gttcgacggt aaaccgttcc agtctgtgtc taaagttgac gagtcgctgg
    15481 aaaaactggc tgacgaagtt gatgagagcg cgaaagaagc ggagaaagca ctgactccgt
    15541 tcatcgaccg tgtgaaagcc ctgctcggcg agcgcgtgaa agatgtccgt ctgactcacc
    15601 gtctgaccga tacgccagcg atcgtttcga ccgacgcgga cgaaatgagc actcagatgg
    15661 cgaaactgtt cgctgcggcg ggccagaaag tgccagaagt gaaatacatc ttcgaactga
    15721 acccggatca cgtactggtg aaacgtgcgg cggatactga agatgaagcg aagttcagcg
    15781 agtgggtaga actgctgctg gatcaggcgc tgctggcaga acgcggtacg ctggaagatc
    15841 cgaacctgtt tattcgtcgt atgaaccagc tgctggtttc ctgatgtcat gccggatgac
    15901 cttcgtgtca tccggcattt ttcttttctt catctgcact ttccgcaaat tatcttgcca
    15961 ttaaccgttt cagcctcagg tgcctttctt gaggcaatcg cctgttggtg gtatcgttta
    16021 tcgctttttc aaaaaattcg acacatttta aggggatttt cgcaatgcgt atcattctgc
    16081 ttggcgctcc gggcgcgggg aaagggactc aggctcagtt catcatggag aaatatggta
    16141 ttccgcaaat ctccactggc gatatgctgc gtgctgcggt caaatctggc tccgagctgg
    16201 gtaaacaagc aaaagacatt atggatgctg gcaaactggt caccgacgaa ctggtgatcg
    16261 cgctggttaa agagcgcatt gctcaggaag actgccgcaa cggtttcctg ttggacggct
    16321 tcccgcgtac cattccgcag gcagacgcga tgaaagaagc gggcatcaat gttgattacg
    16381 ttctggaatt cgacgtaccg gacgaactga ttgttgaccg tatcgtaggc cgccgcgttc
    16441 acgcgccgtc tggtcgtgtt tatcacgtta aattcaatcc gccgaaagta gaaggcaaag
    16501 acgacgttac cggtgaagaa ctgactaccc gtaaagacga tcaggaagag accgtacgta
    16561 aacgtctggt tgaataccat cagatgactg caccgctgat cggctactac tccaaagaag
    16621 cggaagcggg taacaccaaa tacgcgaaag ttgacggcac caagccggtg gctgaagttc
    16681 gcgcagctct ggaaaaaatt ctcggctaat caagccgcag cagatctgct ttcgggcagg
    16741 tctgctcatc ctcctcaggc aatacctctt acagcaatta gttcttcttc ttcacttttc
    16801 cgctacaatt atcaacaagt tgaatcgata agaggcggta atgcgtcaaa ctaaaaccgg
    16861 tatcctgctg gcaaacctgg gtacgcctga tgcccccaca cctgaagcgg taaaacgcta
    16921 tctgaaacaa tttttaagcg acagacgcgt ggttgatacc tcacggttgt tatggtggcc
    16981 gttgctgcgc ggcgtgattt tgccgctgcg ttcgccgcgt gtggcgaagc tgtatgcctc
    17041 tgtctggatg gaaggtggct cgccgctgat ggtttacagc cgccagcaac agcaggcgct
    17101 ggcgcaacgt ttaccggaga cgcccgtggc gctgggaatg agctacggct cgccatcact
    17161 ggaaagcgcc gtagatgaac ttctggcaga gcatgtagat catattgtgg tgctgccgct
    17221 ttatccgcaa tactcctgtt caacggtcgg tgcggtatgg gatgaactgg cgcgaattct
    17281 ggcgcgcaaa cgtagcattc cggggatatc gtttattcgt gattacgccg ataaccacga
    17341 ttatattaat gcactggcga acagcgtacg tgcttctttt gccaaacatg gcgaaccgga
    17401 tctgctgttg ctctcttatc atggcattcc ccagcgttat gcagatgaag gcgatgatta
    17461 cccgcaacgt tgccgcacaa caactcgcga actcgcttcc gcactggaga tggcaccgga
    17521 aaaagtgatg atgacctttc agtcgcgctt tggccgggaa ccctggctga tgccttatac
    17581 cgacgaaacg ctgaaaatgc tcggagaaaa aggcgtgggt catattcagg tgatgtgccc
    17641 gggctttgct gcggattgtc tggagacgct ggaagagatt gccgagcaaa accgtgaagt
    17701 cttcctcggc gctggcggga aaaaatacga atatattccg gcgcttaatg ctacaccgga
    17761 gcatatcgaa atgatggcta atcttgttgc agcctatcgc taaagaatcg agcaccgtcg
    17821 cgtagagcat cgtcggcggt tttcatcatc cgtgaataat gcaaaaaggc gtgcagcata
    17881 ccggagtaga gtttgaactc acaaggttgc tggtgtgccg ctaacgtctg gtaaagcaga
    17941 cggctgtcat ccagcagtgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg
    18001 cgagtgagat cattattaaa caggcagtaa tatggcgact cgcggtccgc gtcgttgctt
    18061 aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccaccc
    18121 aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacgccc
    18181 gcaactttac cgcaatcgat ctgtttatcg cgcaaccaca acgcactggc gagcgccagc
    18241 atggcacctg cggaatcacc agcaaaacca atgcgggaca tattgatttg ataatcctcc
    18301 gcctgctggt ggaaataaca acaggcagcc acaatttcct ctatcgcttg cggaaaacgc
    18361 gcttcaggtg aaagggtgta atcaatgcca atcaccgtac attggctgta gcttgccagc
    18421 aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa
    18481 taaaatagcg tcgccgggct atctggctgc ggataaaaga tacgtgtttt cacctgccca
    18541 tattttgttg gaaccctgta agctctggtt gccatttctg gagcgcccac attccagaat
    18601 cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc aggccagggc
    18661 ggtaaatccg gctgaagagt attcacaacg gtcttcattt cagcagagat aaggtccaga
    18721 acgggtaatt tgttttccgg cttcatgcaa aaactccttt caaattacgt cattgtaagg
    18781 aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc
    18841 gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg
    18901 aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaacgcacg cgatccgctg
    18961 cttcagcaat tccagccaga aaacgaaacc agcgctgcct gggtagtggg tatcgatcaa
    19021 acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc
    19081 gccgggcatt cactggtgat tgaggatgac gttgccgaag cgctttatca ggaactaaaa
    19141 cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcacaac
    19201 tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa
    19261 attggcagct atgcctatcg ttacctgtgt aacacctcca gccgtatcga tcttaactat
    19321 ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa
    19381 cgtacctttg ctatcagccc gggccacatg aaccagctgc gggctgaaag tattccggaa
    19441 gatgtgattg ccggagcctc ggcactggtt ctcacctctt atctggtgcg ttgcaagccg
    19501 ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta
    19561 ccggtggtgc tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag
    19621 caattcctca aagaccacgt ctctatcctt gcgatgaacg aagatgaagc cgaagcgttg
    19681 accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg
    19741 ctgtgcaccg ccgggccaat cggcttgtat atggcaggct ttaccgaaga cgaagcgaaa
    19801 cgtaaaaccc agcatccgct gctgccgggt gctatagcgg aatttaacca gtatgagttt
    19861 agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtctattc gcacattgcg
    19921 ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcagggga tggcgcactg
    19981 gcagcgttgc tgcatgacat taccgccaac agctaccacc gtagcaacgt accaaactcc
    20041 agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct
    20101 aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg
    20161 gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag
    20221 gccagataag acgcgcctat tatccggcaa ccaccgttta ccccgtcacc acctcacccg
    20281 ccggtggcgt ttccagcagt tccagcatag tacgggcaat ttcacgctca cccatcacta
    20341 cctgattcgc accacgttcg gtgatatacg ccacttcatc gtcataatgg gcgcgggcaa
    20401 taatctcaat atccggattt ttcgcgcggg cagatgccac aatctcgccc gcttcatagc
    20461 cgttgggaat cgtcaggatc agccattttg cacattccag atgcgccagt tgcataattt
    20521 cttcgttcgc cgcattgccc aatactgcgc ggaccccgcg ctcgcgcagc tcatcaacac
    20581 gggttcgtga cgtctcaatc accaccagcg gaatatcaga ggcgagcaat ttctccccca
    20641 gcaggctgcc tacacgaccg taacccacca gtagcgcatg gttgcaaata tccactggga
    20701 tctgcttctc ttcttcgatt gcctcttcca gcgtctgctc ttccagcgtt tcggtcttcg
    20761 ccagatattt ttccagtagt gcgaacagta ccgggttgag cataatcgac aggatcgccc
    20821 ctgccagtac caggttttgt ccggcctgcg gcagtaaatt taatgccatt cctagtcccg
    20881 ccaggataaa cgcgaactca ccaatctgcg ccaggctggc ggcgatggtt aacgccgtgc
    20941 gctgggagtg accaaacaaa cgcacaagga acagggccgc gaccgattta ccaaacagaa
    21001 taattgccag cgtcgccagt actgccagcg gttgctgaat cagaattaac ggatcaaaca
    21061 acatcccgac ggagacaaaa aacagcaccg caaacgcgtc gcgcaatggc agtgtatcgt
    21121 gggcggcacg gtgactcagt tcagactcgt tcagtaccat cccggcaaag aacgcaccga
    21181 gtgcaaagga gacatcaaac agctctaccg caccaaaggc aatccctaac gccagcgcca
    21241 gcaccgacag ggtaaacagc tcgcgggaac cggttgctgc gctgcgtgcc ataatccacg
    21301 gcaccagacg gcgacctacc agcatcataa tggcgataaa tgcgatcacc ttgccgatgg
    21361 tgatccccat atcgactgca agagtggcaa agcccacatc gccctgttcc atcattcctg
    21421 ctaccgcggg tagcaatacc agtgtcagga ccatcaccag gtcttccaca atcaaccaac
    21481 cgatggcgat ttgcccacgc tgactgtcaa ttaattgccg ttcttcaagt gcgcgcagta
    21541 acaccacggt actggcggtg gaaagacata agccgaacac gataccggtc attaacgacc
    21601 agcccagcac ggcggagagc gccataccca gcagcgtcgc cacggctatc tgggcgatcg
    21661 cgccgggaat ggcgatggac tttaccgcca tcaaatcctt gagcgaaaaa tgcaaaccga
    21721 cgccgaacat cagcaaaata acgcccagtt cagccagttc cggcgcaagc ttggtatcgg
    21781 caacaaagcc cggagtgaat ggtcctgcca gcacacccgc taacagatat cccaccagag
    21841 gagaaatacg tagtttattg gccagcatgc cgaggataaa ggcgagcaca aggccgccaa
    21901 caatggtggt gataagcggg gtggcgtgat gcattccgtc tccttttcct ggtggttatt
    21961 gtccattttt ggccgggaaa accaaaatta caggtaatag tttatgacaa tttcattgat
    22021 gatgtttatg aataattgtt gaattttgca gaaaaatgga attagctgca aaaaaagcac
    22081 ggatcggaaa acagaaggcg gtttaacaga ggaaaggctt atggcgactg taaaacgatg
    22141 cagccaaagt ttgcctttgg ctgcaatgaa atcagtcttt atgccggtta tcaggcagga
    22201 atatggtcaa catccccaat agtggcagga aagcacagat tttatagact aactctatgc
    22261 tggtgtggtc ggcgataagc cccagaactg ccgcccccag acctcccatg ccgaaagcaa
    22321 aaccgaaaaa cagtccagaa accataccga tacgtcccgg aagcagctcc tgggcgtaga
    22381 ccagaatggc agagaatgct gaagcgagga taaatccaat aatcaccgtt aaaacccccg
    22441 tccagtgcag gctggcgtag ggtaaaatca gcgtaaacgg cgcaacgccg aggatagagc
    22501 cccaaatcac atatttccgt ccaattttat cccctacagg cccgccaatc accgtacctg
    22561 ccgcaacggc aaacaggaag gcaaacaaat gaagctgagc attctggata gataatccga
    22621 atttttgcat cagataaaag gtgtaatagc tgctgatgct cgccatatag aaatatttcg
    22681 agaaaatgag gattaacaga atgctgaccg ccagaacaac tttattgcgc ggcagtggat
    22741 tgataatcgt cgctttgggt tttcctttat tcattcggtg ctgtgccgag taccaacggc
    22801 tgatttgcgc caacaccacg atcgccagca gtgccgcaag cacaaaccag gcaacgttgc
    22861 ctttgccata aggcgcgata atcaccgccg ccagcaaggg gcccagggaa ctgccaaagt
    22921 tgccgccgac ctgaaagata gattgcgcca ggccatgacg cccgccggaa gccatacggg
    22981 ccacgcgaga agattccgga tgaaagaccg atgaaccggt accgaccagc gccgctgcca
    23041 gcagaactgc gccaaaactg cccgccagcg ccagcagcac cagaccactt aaggtaaaac
    23101 acatgccaat cggcaacgac cacggcatcg gatatttatc ggtccagtag ccgaccaccg
    23161 gttgcagtag cgaagaggcg agctggaagg tgagggttat catgccaatc tgcataaatg
    23221 tcagagaaaa ttctgactga agcagtggat aaatcgccag aatcagcgat tggatcatgt
    23281 cgttcagcag atgtgagagg ctgatagcac ctaaaatacc aaacgatgtt cgggccttag
    23341 tcgttgacgc agccgcgccc gccacaggct gggtttgttc actcattgcc ataggaaagt
    23401 cactttttca gggttgcgat gtaaagaatg atcttatttg tgattattac ccgactaaca
    23461 tacctgtatg cgccgcctga aggaagtctc aacgcggaat acagaatttc taatctggat
    23521 gcagatttat cttcaccgga cgcagacttg tttatgatgt cgcgtcatac tatttttcaa
    23581 cacgttgaaa tcaggtcagg gagagaagta tgaaattatt gcagcggggc gtggcgttag
    23641 cactgttaac cacatttaca ctggcgagtg aaactgctct ggcgtacgag caggataaaa
    23701 cctacaaaat tacagttctg cacaccaatg atcatcatgg gcatttttgg cgcaatgaat
    23761 atggtgaata tggtctggcg gcgcaaaaaa cgctggtgga tggtatccgc aaagaggttg
    23821 cggctgaagg cggtagcgtg ctgctacttt ccggtggcga cattaacact ggcgtgcccg
    23881 agtccgactt acaggatgcc gaacctgatt ttcgcggtat gaatctggtg ggctatgacg
    23941 cgatggcgat cggtaatcat gaatttgata acccgctcac cgtattacgc cagcaggaaa
    24001 agtgggctaa gttcccgttg ctttccgcca atatctacca gaaaagtacc ggcgagcgcc
    24061 tgtttaaacc atgggcgctg tttaagcgtc aggatctgaa aattgccgtt attggcctga
    24121 cgacggatga cacagccaaa attggtaaaa cggcatctgg attgtgcagg cgcatgagtg
    24181 gggcaaatac gtgggacggg ctgattttga gtttcgtaat ggcgaaatga aaatggttaa
    24241 ctaccagctg attccggtga acctgaagaa gaaagtgacc tgggaagacg ggaaaagcga
    24301 gcgcgtgctg tacacccctg aaatcgctga aaaccagcaa atgatctcgc tgttatcacc
    24361 gttccagaac aaaggcaaag cgcagctgga agtgaaaata ggcgaaacca atggtcgtct
    24421 ggaaggcgat cgtgacaaag tgcgttttgt acagaccaat atggggcggt tgattctggc
    24481 agcacaaatg gatcgcactg gtgccgactt tgcggtgatg agcggaggcg gaattcgtga
    24541 ttctatcgaa gcaggtgata tcagctataa aaacgtactg aaagtgcagc cattcggcaa
    24601 tgtggtggtg tatgccgaca tgaccggtaa agaggtgatt gattacttga ccgccgtcgc
    24661 gcagatgaag ccagattcag gtgcctaccc gcaatttgcc aacgttagct ttgtggcgaa
    24721 agacggtaaa ctgaacgacc tgaaaatcaa aggcgaaccg gtcgatccgg cgaaaactta
    24781 ccgcatggcg acattaaact tcaatgccac cggcggggat ggctatccgc gccttgataa
    24841 caaaccgggc tatgtgaata ccggctttat tgatgccgaa gtgctgaaag cgtatatcca
    24901 gaaaagctcg ccgctggatg tgagtgttta tgaaccgaaa ggtgaggtga gctggcagta
    24961 atccgaaagt gccggatgtt cgcatccggc acaatgctta atcgcgacgg gcgatatcag
    25021 caaatttggc atcgagtatc tttgccaggt cgcttgccgc cagttcgata tccagaccgc
    25081 gcttgccgcc ggaaacataa atagtggcaa attcttgtgc gggggcgtcg ataatcgttg
    25141 gcagacgttt tttctgcccc agtgggctaa tccctccaac cagatatccc gtcgaacgct
    25201 gcgcgaccat cggatcggcc atctcaactt tcttggcacc cagcgctttt gctacttttt
    25261 taagatccag ttgacctgcg accggcgtaa cggccacggc aaggtgtttc atatcaccgt
    25321 tcactgcgac cagcagcgtt ttgtagacct gatccggatt caaacctaat tttttgacga
    25381 cttcatcgcc aaaattggtt tcagccggat cgtgctcgta ggtatggatt tgaaacgaaa
    25441 tcttgttttt ttcgagtaat ttaactgcgg gtgtcatggt aattttcctg ccttaaacaa
    25501 aaaaacgaag caagcatacg cctgatggcg gtccaaaaaa aagagtcatc ttgcctaaga
    25561 gtattggcag gatggtgaga ttgagcgaca atcgagttac accgtcgctt aaagtgacgg
    25621 cataataata aaaaaatgaa attcctcttt gacgggccaa tagcgatatt ggccattttt
    25681 ttagcgcaac atctgcggca aattcccttc tccatacagg tgtagtgcac cgactgcgac
    25741 cacatagcgc cccggcggca tggcgcgtaa tttatcccgc caggcgagat ttcgctgatg
    25801 catcagcaca tcgtacagcg actgactgaa cgtattgggc agcgttattt cattattttg
    25861 cggcggcgca ttcaaccacc agctcatcat ttgttgcagc aaccgtgcat tggtgtgcca
    25921 gtgggtcagc gtgtcgtcca gcagcgccag tccgttgtca gggagctgga gcaacatggc
    25981 aatctggttt tcagcccctt ccagttcaat cacgggttta tgttgttgct tcgccgcctg
    26041 caatagctgg taatcgatac cgtattctgc ccgcagcccc agtttttgtg cctgcgtcgc
    26101 ctgaagaacc atcgcgattt gccacagcgg ttgagtagaa aagagcgacg gagaaatgcc
    26161 catctcctgg ctaatatgct gcagattttg gagttgctcc tcgctaatgc gctcttccag
    26221 cgtctcgcag gcaggcaaat tagcaaaagg cgtatcgctg gtggaaacat ccgcctcgac
    26281 gatcagcgca tcggcgtttt tgagcttttt gagcaaacgg gtgggcaggg gagccatatc
    26341 gtggctaccc atatgaatac tgccgatcag atgaaaatgg cgattgccgg gaagagagat
    26401 atcgatagca ggccaggtgt aatgattacc gcgcagcgcg gcccaaagcg tttttacccg
    26461 gtacaacaga tccatacgac ctccctttgt gaaatatcat gctagcgcgc ggggagggat
    26521 ggcgcaaatg gagttgattg ttgcacttgt acacagtgct tgtgtgcatt acaatgatta
    26581 aataatggac aaggagatga gagaggaatg gctcaaaaga aaaatataag gagttttcgt
    26641 gatgcctggt tggctgattt tttgtccatt caacgccaca tcgaaagatc cctgctgaaa
    26701 tccataccac gttatcacga aagttggaca tcattaatgc tgccacttct catcgggatt
    26761 tacgctcccc tccggggaac cgatacgaag agttatccgg gaaattacag gaatactcct
    26821 ccattagagt gaataaacag taccgactga tttttaaatg ggttaatggc aaggccgaag
    26881 aattgttttt agacccacac aactactaaa acatagctgt cgtattgacg agaaaggtaa
    26941 aactatgaaa caggcaacaa gaaaaccgac gacacccgga gatattcttc tctatgaata
    27001 tctggaaccg ctcgatttga aaatcaatga gttagcagag ttgctgcatg ttcatcgtaa
    27061 tagcgtcagt gcactgatca ataacaatcg taaactcacc actgagatgg cattccgtct
    27121 ggcgaaagtt tttgatacca cagtcgattt ttggctaaac ctccaggcgg cagttgatct
    27181 ttgggaagtt gaaaacaaca tgcgtaccca ggaagaattg ggacggattg aaacagtggc
    27241 tgaatatttg gcacgccgtg aagagcgtgc aaaaaaggtc gcgtagaata atttccctat
    27301 aacggatgcg ccaccctaaa gcagcgcatc cgcaatgatg tacttattcc ttcggtttaa
    27361 accgcagcaa tcggttggcg ttactcacta cggtgatcga cgagagcgcc attgccgctc
    27421 cggcaaccac cgggttaagc agtgttccag tgaacggcca caaaataccg gcggcgaccg
    27481 gaataccaat gctgttgtag ataaacgcac cgagcaggtt ctgcttcatg ttgcgcagcg
    27541 ttgcgcggga aattgcgagc gcatccgcaa cacccatcag gctatggcgc atcagggtaa
    27601 tcgccgcggt ttcaatggca acatcactgc cgccacccat cgcaataccg acatccgcct
    27661 gagccagcgc tggcgcatcg ttaatgccgt ctcccaccat tgccacctga cgtccttcac
    27721 tttgcagacg tttgatcgct tcggctttac cgtctggcag cacgccggct atcacctcat
    27781 caatccctgc ttctttagcg atcgcattgg cggtggtcgg gttatccccg gttagcatca
    27841 ccaggcgata tcccgcttta tgcaggcgtt gcagcgccgc cacgctatca ctacgcaacg
    27901 gatcgcgtac agccagcagg gctactgctt tcccgtcaac cgccagcagc acaggcgttg
    27961 ccccttgcga tgcctgagct gaaatatctg cttcaattgc tttggtattg acctgttggt
    28021 catttaacaa cgcctgattg cccagcaata acgcatgacc ttcagcttca ccgctcacgc
    28081 ccagcccgcg caatgtgcgg aaaccgttga cctgcggtag ctgcatatca cctgctttat
    28141 cgaggatcgc ccgtgccaga gggtggctgg aaccttgctc cagcgccgcc gccagacgca
    28201 atgcttgcgc ttcatcaata tcagcaaatg tttttactgc gacaacctgc ggcttccctt
    28261 cggtcagcgt cccggtttta tcgaacacta cggtgtcgag cgtactggcg cgttgcagcg
    28321 cgtcggcgtc ccgtaccagc acgccaaact cagccgcccg cccgacgccg gaaataatcg
    28381 acatcggcgt cgccagtccc agcgcacacg gacaggcaat aatcagcacc gtggtggcaa
    28441 tcaccagggt atagacaatc tgcggtgccg gaccaaagaa ataccagatt gccgcactga
    28501 tcagcgcaat caccaccact accggcacaa atacggctga gattttatcc gccagctgac
    28561 cgatttctgg cttactgctc tgggcctggc gcaccatacg aataatccgc gacagcgtag
    28621 tatggctgcc aaccgcactg gcgcgaaaca gcacgctacc gtcctgcacc actgtcccgg
    28681 catggacgct atcgccttcg cctttttgct gcgggatagg ttcgcccgtc agcatcgctt
    28741 catccagcca tgcttcgccc tgggtaatct cgccatctac tggcacgcga tcgccggtcg
    28801 tcaggcgcag caacatacct ggttgcactt ccgccagcgg cacgcttttt tcaccttcgt
    28861 cagtaaccag gcgcgccgtc ggcggagtta aatcgagtaa tttctccagc gcctttgaag
    28921 aacgctgacg tgcgcgcgct tccagcatat gaccgagatt aatcagaccg ataatcatcg
    28981 cactggcttc atagtaaaga tgccgtgctt ccatcgggaa ccactgcggc cacaggttga
    29041 cgctcatcga atagagccac gcaacacccg ttcccagcgc caccagcgta tccatcgtcg
    29101 ccgcaccgtt cagcaggctt ttccatgcgc tgcggtaaaa atgcccgccg gcgaaaacca
    29161 ttaccgccag ggttatcagg ccgataacta accacaggct gcggttgtca gcggtgacca
    29221 tcatgttatc gccgatcatc ccccagacca tcaccgggat acccaccacc agcgcgacaa
    29281 ttgcctgcca gcggaagcgc ttcatcgtag cgacggcggt ttcttgctgg cgctcgcggc
    29341 gtttagcgtc atcttcaatc gcttccgcgc cgtagcccgc tttttccacc gcctgcacta
    29401 aatcttgtgg ggaggcactg cccatcacca gcgcagtacg ctccgccagg tttacccgtg
    29461 cctgagtgac gcccggtacg ctttgcaacg cattttgtac gcgggtgaca cagctggcgc
    29521 agctcatgcc gctcagcagc aactgctggc tgtcatcgtc atcggcggtc gctgccggaa
    29581 gcgcctcaga aaccgctgtc agtgcttccg acgggattga tgactccgcc agcggtttag
    29641 cctttgggtg gcttacagat gcgtcataac ccgcttgttt gatggtttcg atcagctgtt
    29701 ctgcactggc agtcccggta acgtgcgctt cagtgataga cacatccgcc tgttcaacat
    29761 ccgggcgctg ttcgagactt tctttcacgc gtttaacgca gtgaccgcag gacaggccat
    29821 ccagggtcag gtcgatagtt tgtgacataa aacactcctt taagacagtt ttgactggct
    29881 gtgataaagg ttaaaccttc cagcaagggg aaggtcaaga aattaataaa ccaggcgggt
    29941 aaaagtccgt aaagattaaa aaatcggctc gatttgcatc aggattagac atttatctct
    30001 ttgttttcct gtagttaagt tgcgggtgct aagttaaatc aggatgcatg aaaatcggca
    30061 ccggggtgag gaattacctc ccgcatctat aaaaaggagt taacaaaaga tgttagatgc
    30121 aaacaaatta cagcaggcag tggatcaggc ttacacccaa tttcactcac ttaacggcgg
    30181 acaaaatgcc gattacattc cctttctggc gaatgtccct ggtcaactgg cggcagtggc
    30241 tatcgtgacc agcgatggca acgtctatag tgcgggtgac agtgattacc gctttgcgct
    30301 ggaatccatc tcgaaagtct gtacgttagc ccttgcgtta gaagatgtcg gcccgcaggc
    30361 ggtacaggac aaagttggcg ctgacccgac cggattgccc tttaactcag ttatcgcctt
    30421 agagttgcat ggcggcaaac cgctttcgcc actggtaaat gctggcgcta ttgccaccac
    30481 cagcctgatt aacgctgaaa atactgaaca acgctggcag cgaattttac atatccaaca
    30541 acaactggct ggtgagcagg tagcgctctc tgatgaagtc aaccagtcgg aacaaacaac
    30601 caacttccat aaccgggcca ttgcctggct gctgtactcc gccggatatc tctattgtga
    30661 tgcaatggaa gcctgtgacg tgtacacccg tcagtgctcc acgctcatca atactattga
    30721 actggcaacg cttggcgcga cgctggcggc gggtggtgtg aatccgctga cgcataaacg
    30781 cgttcttcag gccgacaatg tgccgtacat tctggccgaa atgatgatgg aagggctgta
    30841 tggtcgctcc ggtgactggg cgtatcgcgt tggtttaccg ggcaaaagcg gtgtaggtgg
    30901 cggtattctg gcggtcgttc ctggcgtgat gggaattgcc gcgttttcac caccgctgga
    30961 cgaagaaggc agcagtgttc gcggtcaaaa aatggtggca tcggtcgcta agcaactcgg
    31021 ctataacgtg tttaagggct gatcattatg aacacggaag gtaataacgg taacaaaccc
    31081 ctcggtctat ggaacgtcgt ttccatcggc attggggcaa tggtgggggc ggggatcttc
    31141 gcgctgctgg ggcaggctgc gttgctaatg gaagcctcga cctgggtcgc ctttgcattt
    31201 ggcggtattg tggcgatgtt ttccggttat gcctatgcgc gcctgggggc gagctatccc
    31261 agcaatggcg gtattatcga cttctttcgt cgcggattag gcaacggcgt cttttcgctg
    31321 gcgctctcgt tactgtacct gttgacgctg gcggtaagca tcgccatggt cgcccgtgct
    31381 tttggcgctt atgccgtgca gtttttgcat gaaggcagcc aggaggagca ccttatattg
    31441 ctctacgcgt tggggatcat tgcggtgatg acgcttttca actccttaag caaccatgcg
    31501 gtagggcggc tggaagtgat cctcgtcggc attaaaatga tgatcctgtt attgctgatc
    31561 attgcaggtg tctggtcgct gcaaccggcg catatttccg tctctgcgcc ccccagctcc
    31621 ggtgcgttct tctcctgtat cgggataacc ttccttgcct atgcgggctt tggcatgatg
    31681 gcgaacgcgg cggataaagt gaaagatccg caggtcatta tgccacgggc gtttctggtg
    31741 gcgattggcg ttaccacgtt gctttatatc tcgctggcgc tggttttgct tagcgatgtg
    31801 tcggcattag agttagaaaa atatgctgat accgccgtag cgcaggctgc ttctccgctg
    31861 ctcgggcatg tgggttatgt gatcgtcgtc atcggcgctt tactggcgac ggcttcagcc
    31921 attaacgcga acctgttcgc cgtgtttaac atcatggaca acatgggcag cgaacgcgaa
    31981 ctgccgaagc taatgaataa atccctgtgg cggcagagta cctggggcaa catcattgtc
    32041 gtggtgctga ttatgctgat gacggcggcg ctgaatttag gctcactcgc cagcgttgcc
    32101 agcgccacct ttttgatctg ctatctggcg gtgtttgtgg tggcgatccg cctacgtcat
    32161 gatattcacg cctcgttgcc gattcttatc gttggtacgt tggtgatgtt gttggtgatc
    32221 gttggcttta tctacagcct gtggtcccag ggcagccgtg cgttgatatg gattattggt
    32281 gcaatcttac tcagccttat tgtggcaatg gtcatgaagc gcaataaaac cgtataacat
    32341 ctctctgtgc gcagtacttc ctgtattatt gtggtggcgg tcgatattcg cactggcaaa
    32401 aaaacgtgct tgaatatttg ttgaaaccct ttaacaaagc acaggaggcg ttgcgcgaac
    32461 gatgaacatt agcgatgtag caaaaattac cggcctgacc agcaaagcca ttcgcttcta
    32521 tgaagagaag gggctggtga cgccgccgat gcgcagcgaa aacggttatc gcacctacac
    32581 gcagcagcat ctcaacgaac tgaccttact gcgccaggca cgccaggtgg gctttaacct
    32641 ggaagagagc ggcgaactgg tgaatctgtt taacgatccg cagcgtcaca gcgccgacgt
    32701 caaacgtcgt acgctggaga aagtagcgga gatcgaacag catatcgaag aactgcaatc
    32761 catgcgtaac cagctgctgg cgctggcgaa tgcctgcccc ggtgatgaca gcgccgactg
    32821 cccgattatc gaaaatctct ccggctgctg tcatcatcgg gcggggtgat taaggcttgc
    32881 gaaaatagtc gtccattatt ttcgtcagct ccggacgttc cccgtcatca ttgatggcaa
    32941 aatccagaaa aaaagcatct gcttgcggcg ttcggttttg cgccatcaac cggaaggtga
    33001 gggggcgata aatggcgtat gccggatggc tgataacgaa tcgggcaaag tcaataaaaa
    33061 gcggcgagtg tgtggaaaac agcgcctgca aaataatatc agccttttca tcctccccca
    33121 aaccttgttg gatcatgctc ttaatacgag tttcgctcaa attcccggcc tcaacaattt
    33181 gccaggcttg atccagcgag gggtaccgac tgacgctctg ctttaactct tctgaccagg
    33241 tttcggatgg gctgatgtgc ttatcagggc cgaaactctc ccatgcgctg ggcggtaatg
    33301 ttttgcacgc ggtgagtttt ggtatgtggt attgcagctc tgttttgtgc agacattccc
    33361 cgccaggaaa taccagggtt tgttgctcat cattaaaatg ggttgccgtg ctgagcacca
    33421 gtgttttgag cggtcgccct tccagcgcgg cggcagtagc cttatggtgt ccgtcgagca
    33481 gaacgcagag ataggaatca gggagatacc aggccagagc gcgggcgtcc ggtttatcgc
    33541 gataaaacgt cgcacgctca ggattaaaat gtgatggcgg ctggccgggt agaagaaagc
    33601 agggtgcggt gtcactgaaa gagcaatatt caggatccca gacggcaacg gttgccggat
    33661 tactggaact atggtccgtt gccgcccaaa agaaatgccc gttgccatcc gtcgggtaaa
    33721 gttcacgatc ttccagcaaa taataaccat cagccatgca tgagaacggc ggtgtcagcg
    33781 cggcaaaagc gtccgttatg ccagaccacg ggcgattcca gttttcgaga gcagacaaaa
    33841 accgggcggc tccctgttca tcctccgcaa aaccgctgta gacaaattcc gcacaggtag
    33901 ggcatccagc ctcggaccag aaacgtatta acgggacatc ctgccagcag agcgaatgga
    33961 taagaccgtg cgagtgcgga tcgtaaatca cacattgacg cagaaatcct ttcccggact
    34021 tcaccgaaaa aatgacgccc gcagcggtat ttttcagcag acgtacttca ggttttcgaa
    34081 agggggaaaa catggctact ccttagccga acattcaatt aacactatgt agggggctat
    34141 atgccggatg cggcgtaaac gccttatccg gcatggggtt tggtacagat ttccaggtgt
    34201 gataagacgc gtcaggcgat gaccgcacgg ataatcagcg ttattccttc tatcgcaatc
    34261 acctcaacat gcgtaccagt gccgagatcc tcgctggcgc tgacaggcca tgaactgtca
    34321 ccgacgcgca tatgaccgcg cccgttgacc agcggagatt ccagaacaaa acgtcggcca
    34381 atcaactgct gcccgcgctg gtttaaatga ctgtcgctgt gcttttgttc gcgcacccgc
    34441 cgcgacaacc atttccacca cagccaggcg gcgagcagcg tcaggacggc aaacattacc
    34501 ccttgccact cccaacccag cggcagcaac cagaccacca gaccggtaat caccgctgcc
    34561 acgccgctcc acaacagata accattgccg cccaacatct cggctgccag cagcaaaccg
    34621 ccgagactta gccagaaaat atgtggatga acgaccatta actccatcat ggctgagtcc
    34681 gtttgttggc gctgtctttc accagctcag caatcccggc aatcgacccc atcaggctgc
    34741 tggcctctaa tggcatcatc accactttgc tgttactgga cgaaccgatc tgctgtaacg
    34801 cttcggtgta tttctgtgcg acgaagtagt tcaccgcctg aatatcaccg gaggcgatgg
    34861 cttcagacac cattttggtg gcgcgggctt ctgcttcagc ggaacgttca cgcgcttcag
    34921 cctgtaaaaa cgccgactga cgttcgcctt ccgctttcag gatttgtgac tgtttttcac
    34981 cttcggcttt gaggatttcc gcctgacgga tcccttccgc ttcaaggatg taagcgcgtt
    35041 tggtacgttc cgctttcatc tgcgcgttca ttgaagagat aagctctgcg ggcgggcgca
    35101 catcacgaat ttcgatacgg gtgactttaa tcccccacgg gttggtggcc tcatcgacaa
    35161 tatgcagcag acgcgagtta atgctgtcgc gctgagagag catttcatca agttccattg
    35221 aacccagcac ggtacggatg ttagtcatgg tcaggttgat gatcgccagc tccagattgc
    35281 tgacttcata agccgcgcgc ggcgcgtcaa tcacctgaat aaagcacaca gcgtcgatgg
    35341 taacgttggc gttatctttc gagataactt cctgggaagg gatgtcgagc acttgctcca
    35401 tcatattgat cttgcgacca atgcgatcca taaacggcac caccaggctt agacctggct
    35461 gtaacgtttt ggtatagcga ccaaagcgtt ctaccgtcca ttgatagccc tgcggcacga
    35521 ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat gagaatcggg ataaagataa
    35581 gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca aaattgctgc ttgattaaac
    35641 aaattatacc tgattactga aagagagttc ccccttattc ctgcgaagga taaactgttt
    35701 ttagtaaaaa tcagaaaaag ggaacagcga tgcaggaaaa tagtcctttg cttcagctac
    35761 aaaacgtagg atatctggcg ggtgatacga agattcttaa taacatcaat ttttcgctgt
    35821 gtgctggcga atttaagtta attaccggtc cttctggttg tggcaaaagt acactgctaa
    35881 aaatagttgc ttcattgatc agcccaacca gcggaacgtt actgtttgaa ggtgaggatg
    35941 tcagcacact aaagccagaa atctaccgtc agcaagtctc ttactgcgcc cagacgccga
    36001 cgctgtttgg cgatacggta tacgataacc tgatttttcc ctggcagatc cgtaaccagc
    36061 agcctgaccc ggccattttt ctcgattttc ttgaacgctt cgctttaccg gacagcattt
    36121 tgacgaagaa tatcgccgag ctatctggtg gtgaaaaaca acgcatctca ttgattcgta
    36181 acctgcaatt tatgccgaag gttttattgc tggatgaaat caccagtgcg ctggatgaaa
    36241 gtaataaaca taacgttaat gagatgatcc accgttatgt acgcgagcaa aatattgctg
    36301 tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc ggataaagtg attacactgc
    36361 aaccgcatgc tggagaaatg caggaagcac gctatgaact cgcataatat tactaacgaa
    36421 tcattagcac tggcattaat gctggtggtg gtggcaatct taattagcca taaagaaaaa
    36481 ctggcgctgg agaaagatat tctctggagc gtcgggcgcg cgataatcca gctgattatt
    36541 gtcggctatg tgctgaagta tattttcagc gtggatgatg ccagcctgac attattgatg
    36601 gtgttattta tctgctttaa tgcggcgtgg aacgcgcaaa aacgcagtaa atatattgct
    36661 aaagctttta tctcatcgtt tattgccatt acggtcgggg cgggaattac cctggcggta
    36721 ctgatcctct ccgggtcgat tgaatttatc ccgatgcagg tgatccctat cgccgggatg
    36781 atagccggca acgccatggt agcggtgggg ttgtgttaca acaatttagg gcaaagggtc
    36841 attagcgaac agcaacagat tcaggagaaa ctgagtcttg gcgcgacgcc gaaacaggct
    36901 tcagcgatat tgattcgcga cagtattcgc gcggctttaa ttccgacggt cgattcagca
    36961 aaaacggtag gcttagtgag tttaccagga atgatgtccg ggctgatatt tgccgggatt
    37021 gatccggtga aggcgattaa atatcagatt atggtgacct ttatgctgct ctcaaccgcc
    37081 agcttgtcga ccattattgc ctgctattta acctatcgta agttttacaa ttcgcgccac
    37141 caattggtgg tgacgcaatt gaagaagaaa tgatgcgatg ccggacatgc agcatcgcat
    37201 ccggcgttgt ggttcatgtg ccgatcaaca aaacatcaat acaacaacgc atacagctgg
    37261 cggcgatact tcgacgccag tgcatcaccc gtacccagcg cagcgaggat ctcctggaat
    37321 gttttacgtg tctgaccatc tgcggcggtg agatctttac gcagatgccc aaacagtaac
    37381 tccagcgcct cttcattgcg cccaacctgg tgcagttgca gtgccagttg cgtcgccagt
    37441 gcggcatctt ctggattctc cgccacctgc tgttgcaact gttgaatttc cggcgtatca
    37501 gccgcctgct tcagcagttc gatttgcgcc accagcccct gatagcgggt gtcctgatcc
    37561 tgcaacggaa tggttttcag cacggcttct gcatcttcag aacggttcag cgcaatcagc
    37621 gtttctgcca acaacaggcc gatttccccg ttctgattcg acaactgcca ggcgtctttc
    37681 agcaatggca gagcgtcggt gtaattgcct tcctgcatca gttgcatcgc ctgctgcgct
    37741 ttcagctctt cttcacgcgg cagcacttta tccagcaggg cgcggatcgc ctcttccggt
    37801 tgcggcccct ggaagccgtc tactggttgc ccgttctgga acagatatac ggtcggaatc
    37861 gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt cgcagtccag cttcgccaga
    37921 ataaatagcc cgttgtactg cgccgcgagg ctttccagaa ttggggttaa ctgcaaacag
    37981 tgctggctac gttcagacca aaaatagaac agcaccggag tggtcatcga ctgttcaaga
    38041 acctgttgca ggttagattc gttaatgttg acaatatttt ctacggacat ggagtcgctc
    38101 tctgttgtcg atattttctt tgacatgggg gcttaagcgc gcgcttcaac tcacccctgc
    38161 aatattttgt ccatcacgcg ccccggcagc aggcgcttaa gcaccattac cgcccaggcc
    38221 accagcgtta ccggatagcg catcttcggc ttctcgctaa taaaagcatg gcgcactttg
    38281 ccgaccaccg cttccggtcc caacgtaaag cgggcggcga tgccgggatt ttcgactggt
    38341 ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag tacgaatggg accaggttcg
    38401 atcaggctga ctttaattcc actgtggcgc agctccatgc gcagtgcgtc tgaccacgcc
    38461 tccagcgcat atttactggc cgcgtaagcg ccacgacccg gtgtggagat taatcccatc
    38521 accgatgagg tcatcacaat acgcccttca ccgtgcggca acatcgcggg taacaggcgc
    38581 atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact gctgttccat ctgcgcgcgg
    38641 ctgatggtgg aaagggggcc atacatgccg aatccggcat tgttaaagat cccatacaga
    38701 caattatcgg tcagggcgat cacttcgtcg gctgcgcgat caacactttc tggcgaatcc
    38761 agatcgatca acacgccggt gaatcccatg ctgttcatgc gctcaacatc atccggtttc
    38821 cggcaacctg ccagcacatg aaaaccctgg cgttttaatt cgagcgcgct ttccaggcca
    38881 attccactgg aacatccggt aattaagacc gatttttgca taactttacc tgtcaggatc
    38941 tccgttgctt tatgagtcat gatttactaa aggctgcaac tgcttcgcca tccagtcggc
    39001 aataaacggc tgggcgtcgc ggttgggatg aataccgtca tcctgcatcc attgtggttt
    39061 gaggtagacc tcttccataa aaaagggcag cagcggaaca tcaaactctt tggcgagttt
    39121 ggggtaaatg gcgctaaagg cttcattata acggcgacca tagtttgcag gcagacgtat
    39181 ttgcattaac aatggttcag cgttggcggc tttgacatcc tgcaatatct ggcgcagcgt
    39241 ttgctcggtt tgttgtggct gaaaaccacg caaaccgtca ttgccgccca gttctaccag
    39301 cacccagcgc ggctgatgct gtttcagcag agccggaagg cgcgccagcc cttgttgcga
    39361 ggtgtcgccg ctgatgctgg cattgactac cgacgtttta ctctgccact tatcattcaa
    39421 caaggcaggc caggccgcgc tagcagacat tcgatacccg gcgctcaggc tatcacccag
    39481 aatcaataac gtgtccgctg cggcggcacg gaaggttaac aggaccagga acaggaaggg
    39541 caaatgccag cggaaaacat tgttgaagtt catcatctta agaagtccgt cggtcagggg
    39601 gagcatgaac tctccatcct caccggagtt gagctggttg tcaaacgtgg tgagaccatt
    39661 gcactggtgg gcgagtcggg atcgggtaag tcaaccttgc tggcgatcct cgcagggctg
    39721 gatgacggca gcagtggcga agtgagtctg gtgggacaac cgctacataa tatggacgaa
    39781 gaagcgcggg caaagttgcg cgcgaagcac gtaggctttg tttttcagtc atttatgtta
    39841 attcctaccc ttaacgcgct ggaaaacgtc gagcttccgg cgctgctgcg tggtgagagt
    39901 agcgcagaaa gtcgtaacgg ggcaaaagcg ttgctcgaac agttaggact gggtaaacgt
    39961 ctggatcatc ttccggcaca gctttccggc ggcgaacagc aacgagtggc gctggcgcga
    40021 gcttttaatg gtcggcctga tgtgctgttt gccgacgaac ccaccggcaa tcttgaccgc
    40081 cagacgggcg ataaaattgc cgacctgctg ttttccctca accgtgaaca tggcaccacg
    40141 ttgattatgg tgacccacga cctgcaactg gcggcacgct gcgaccgctg cttacgactg
    40201 gttaacgggc agttgcagga ggaagcatga ttgcacgttg gttctggcgc gaatggcgct
    40261 cgccgtcgct attaattgtc tggctggcgc taagcctggc ggtggcctgc gtgctggcgc
    40321 tgggcaatat cagcgatcgc atggagaagg gcttaagcca gcaaagccgg gagtttatgg
    40381 cgggcgatcg ggcgttgcgt agttcgcgcg aagtgccgca agcgtggctg gaggaagcgc
    40441 aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc caccatgaca tttgcaggcg
    40501 acacaccgca gctggcgaac gtcaaagcgg tggatgatat ctacccgatg tatggcgatc
    40561 tgcaaaccaa tcccccaggc ctgaaaccgc aggcgggcag cgtattgctg gccccacgcc
    40621 tgatggcact gcttaacctg aaaacgggcg acaccattga cgtgggcgat gccaccttgc
    40681 ggattgccgg agaagtgatt caggaaccgg attccggttt taaccccttc cagatggctc
    40741 cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg agccgtgcag ccggggagtc
    40801 gggtcacctg gcgttataaa ttcggcggca acgagaacca gctcgacggc tatgagaaat
    40861 ggttgttacc ccagcttaaa cccgaacaac gctggtacgg tctggaacag gacgaaggcg
    40921 cgctggggcg ctcgatggaa cgctcgcaac agttcctgct gctttctgcg cttctgacct
    40981 tgctgctggc agtggcagcg gtggcggtgg cgatgaatca ttactgtcgc agtcgctacg
    41041 atctgatggc gatcctcaaa acgctggggg cagggcgagc gcaactgcgt aagctaatcg
    41101 tcggtcagtg gttgatggtg ctgacgcttt cagccattac cggtggggcg ataggcctgt
    41161 tgttcgaaaa cgtgttgatg gtgctgctca agcccgtgct acctgccgct ttacctcctg
    41221 ccagcctctg gccgtggctg tgggcgctcg gcaccatgac agtcatctcg ctgctggtgg
    41281 ggctacgacc gtaccgactg ttactggcca cgcagccttt acgcgtatta cgtaatgatg
    41341 tggtcgcgaa cgtctggccg ctcaagtttt atctgccgat tgtcagtgtg gtggttgtgc
    41401 tgctgcttgc cgggttaatg ggcggcagta tgctgctttg ggcggtgctg gcgggcgcgg
    41461 tagtactggc ttttctgtgc ggtgttctgg gctggatgct gctgaatgta cttcgccgca
    41521 tgacgctgaa atcgctacct ctgcgcctgg cggttagccg cctgttacgt cagccgtggt
    41581 caacgttaag ccagctttcg gcattttcgc tctcctttat gctgctggca ctgttgctgg
    41641 tgttgcgtgg cgatctgctc gaccgttggc aacaacaact gccgccagaa agcccgaact
    41701 actttttaat caacatcgct acggaacagg ttacgccgct gaaagcgttt ctcgcggaac
    41761 atcagatagt cccggaatcg ttttatccgg tggtgcgggc gcggttgacg gcgattaacg
    41821 acaagccgac agaaggcaat ggagatgagg cgctcaaccg cgagctgaat ctcacctggc
    41881 aaaatacgcg gcctgatcat aacccgatcg tcgccggtaa ctggccgcca aaagccgatg
    41941 aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt tgccctcggc gataccgtga
    42001 cttttatggg cgatacccag gagttccgcg ctaaagtgac cagcctgcgc aaagtggact
    42061 gggaaagtct gcggcctaat ttctatttta ttttcccgga aggggcatta gacgggcaac
    42121 cgcagagctg gcttaccagt ttccgctggg agaatggcaa cggcatgttg acgcaactca
    42181 accgccagtt cccgaccatt agcctgttag acattggcgc gattttaaaa caggtcggtc
    42241 aggtgctgga gcaggtaagt cgggcgctgg aagtgatggt ggtgctggtc accgcctgcg
    42301 gtatgttgtt gttgctggca caggtgcagg tgggaatgcg tcagcgtcat caggagctgg
    42361 tggtgtggcg cactctcggt gcgggcaaaa aactgctgcg cacgacattg tggtgtgagt
    42421 tcgccatgct cgggtttgta tccggcctgg tggccgcaat tggtgcggaa acggcactgg
    42481 cggtactgca atcgaaagtg tttgatttcc cgtgggagcc agactggcga ttgtggattg
    42541 ttctgccgtg cagcggtgcg ctactgctgt cgctttgcgg cggctggctg ggtgcgcgac
    42601 tgcttaaggg taaggcgctg ttcaggcagt ttgcggggtg atgacaggtg ttagattgcc
    42661 tgatgcgcta cgcttatcag gcctacgaag atcgtgcaat atattgagtt ttcgaaattt
    42721 tgtaggccgg ataaggcgtt cacgccgcat ccggcagttg tgcgccgatg ccagatgcga
    42781 cgcaggcgcg tcttatcatg cctacgaaac agcactcacc tgtaggctgg ataaggcgtt
    42841 cacgccgcat ccggcatgaa cagcgtgcac agaatccccc atcactgcgc cttaacccat
    42901 tccaccactt ccgcccactc accgcgaaag acaacttttt ccgctttttt ctcaagctga
    42961 tagcgataca tcgggtcgta atattcttca agtaacggca ccagccaggc cagatgaccg
    43021 tcggtgctgc cggtggtgag ttgcgttgtc agcgccgcat ccagccttgc agccagttcg
    43081 ttatagcgct gtagccccag ccgacgctta atcgccgaaa gtccgtgatg caggtattcg
    43141 caatactcct gccagccctg ttcgtcgccg tacgcgtggg taaaatcatg atgcatacgc
    43201 aggaaatatt cttcgttcag ccgctcaaga cggatctcaa acggatcttc taccaccgca
    43261 atcgtcgcct gagtcattcg ttcgcgcaga cattccggca ggtgattcga accgatcatc
    43321 cggctttcat cttccagcac ccacaagcgc aaattctgac gggcgtcggt ttttagcatt
    43381 tcggcagcca gcaggttttc aaaactcgcc tggctaagtt gtggttgtaa cgtgcgacca
    43441 aaagcagaac cgcgatggcg cgccagccct tccagatcaa caccgttcgg ttgttgttgc
    43501 actaacagcg ttttgccgca gccggtacaa ccgccaatca gcactatcgg tttttgtgac
    43561 agttcaatgg tcgcctgaat cgcggtctgg cgcagtgcct tataaccgcc ttccaccagc
    43621 ggataatcaa tccccgcgtc atgcagccag cgttgcacaa tatgcgagcg ctgaccgcca
    43681 cgggcgcagc agagaatacc gtgcggattt tgcaggcacg ctgcccgcca ggcgtccatg
    43741 cgctgctgac gaatttcacc cgccaccagt ttatgcccca gtgccagcgc cgcgtctgac
    43801 ccttgctgtt tatagcagat gccaacggcg gcgcgttcat cgttattcat taacggcagg
    43861 ttgatagcgg cgggcattgc gccttgctca aactcgatgg gggcgcgaac atcaataatg
    43921 ggcgtatcag caatcagcag ggcacgatag tcctgttccg tgtgtctctc ttgcatagta
    43981 aaaagtgaac ctcaaatcag cttgcgcgct attttacgcg ccaacgcgca aggaaacttg
    44041 atttttaact gcgtgggttg ccgaaaattt ctaaaaatcc gctgatttcc ggcctgcgct
    44101 gggtaaacaa ggtcacaata tcttctaccg ctttgccgct gccaaatttg cgccatgcca
    44161 gactcaatgg cgaaggaggg cgcatcgttg ggattacccg gctgaccagt tgttgattat
    44221 cgagcattga ctggcaaagt gattttggca aaaaaccaat gccaacgccc gccagatggg
    44281 cggcgatttt cgtttccata tcgggaacaa taatctcttt ttgccccggc aatcgccagg
    44341 cgacgcgttt ggttaaggtg cgggcgctgt cttcaatatt gaccgccgga aagcgccgca
    44401 actgtgcttc tgttagcggc tcttcaacgt tcgccagcgg atgatccgcc gccatgacaa
    44461 agcgccattg caccgatcct aagggatcaa gactaaaggt atttgccagc gcctcggttc
    44521 ccgtgacgcc gatagccagt gaaaaacctt cgtacaatag cgagtcccag acgcccatat
    44581 agatttgtcg ggagatgtga aattgggtaa acgggtaacg ttcatttagc cacgccagca
    44641 gcctggcaac ggcctggggg ttatagagca ggttgttgat gacgatattc acctggcgtt
    44701 ccacgccatc attcacctgt tgtaactcgc tgggcatact ttccagccag ctcagccagt
    44761 ctctggcctg acaaagtaga tgctcgccag tcgccgtcaa cgtcacgctg cgagtcgtgc
    44821 ggaaaaacag cgccactccg gtattctctt ccagaagttt aatgcgataa ctgatcgtcg
    44881 ccgtggtttt acataatcgt tctgccgctt ttgaaaaact acctgtttca gcaaccgcaa
    44941 tgaaagtccg caaggtttct ggatcgaaca tcttcaggta tcccctttta aatccgcaag
    45001 ttgcgtgatt ttcttatcct ctgatttatc agtattttta catgataacc ctgttcaatt
    45061 tgtggactaa atctagtttt ggaaaaatat tccaactttt gtgttgatgt tgttctctta
    45121 aggttttaga ttgcctgtta ttgaaaccaa gctgaccggt cggcggtggt tgaacggaat
    45181 tatgttacaa ggacaaaaag atgaaacttc aggtattacc gttaagtcag gaagccttta
    45241 gtgcttatgg cgacgtaatc gaaacgcaga aacgggattt tttccatatt aacaatggac
    45301 tggtggagcg ttaccacgat ttggcgctgg ttgagattct tgagcaagac cgtacgctta
    45361 tcagcattaa ccgcgcgcaa ccggcgaatc tgccgctgac cattcacgaa ctcgaacgtc
    45421 atccgttggg cactcaggcc tttatcccga tgaaaggtga agtgtttgtg gtggtcgtgg
    45481 cgttaggcga cgacaaacca gacctgtcaa cgctgcgggc gtttattacc aatggcgaac
    45541 agggagtgaa ttaccatcgt aacgtctggc atcacccact tttcgcctgg cagcgcgtca
    45601 ccgattttct gaccatcgat cgcggcggca gtgacaactg tgatgttgaa agtattcctg
    45661 aacaggaact ctgttttgcg tgacgcctgc aaccgacttg cataagataa acgaattgtt
    45721 cattgtttat gctcatttgt aggtcggagt taacgtaggt atgacggaag ttagacggcg
    45781 cggcaggcca ggacaggcgg agcctgtggc acagaagggt gcacaggcgt tagagcgggg
    45841 aattgcgatt ctgcaatatc tggaaaaaag tgggggaagt tcgtcggtta gcgatatctc
    45901 tctcaatctg gatttgccgc tctccacgac ctttcgcttg ctgaaggttt tacaggcagc
    45961 ggattttgtc tatcaggaca gtcagttagg ctggtggcat ataggattag gtgtctttaa
    46021 cgtcggtgca gcgtacatcc ataaccgcga tgtactctcc gtcgccgggc cgtttatgcg
    46081 ccgcctgatg ttactttccg gcgaaacggt caatgtcgcg atccgtaacg gcaatgaagc
    46141 ggtattaatt ggtcagttag agtgtaaatc gatggtcagg atgtgtgcgc cgctgggcag
    46201 tcgtctgccg ctgcatgctt ccggagcggg caaggcgctg ctttatccgc tggcggaaga
    46261 agagttgatg agcatcattc tgcaaaccgg tttgcagcag tttacgccaa cgacacttgt
    46321 ggatatgccc accttgctga aggacctgga acaagcgcgt gaactgggct ataccgtaga
    46381 taaagaagag catgttgtcg gcctgaattg catagcttca gcaatttacg atgatgtagg
    46441 tagtgttgtt gccgctatct ccatctccgg gccttcatca agactgacag aagatcgttt
    46501 tgtcagtcag ggtgagctgg tcagagacac cgcccgcgat atcagcacgg cgttgggact
    46561 gaaagcacat ctatagtgtc tgtcgtatcc cgctctgcgg agcgggtttt tttgacaaaa
    46621 tttgaaagtt ggaaaatttt tccaattaat agaggtagga ataaaatggc aaaaatgaga
    46681 gccgttgacg cggcaatgta tgtgctggag aaagaaggta tcaccaccgc cttcggcgtt
    46741 ccgggagctg caatcaatcc gttctactca gcgatgcgta agcacggcgg tattcgtcac
    46801 attctggcgc gtcatgtgga aggtgcttcg cacatggcgg aaggttatac ccgcgcaacg
    46861 gcagggaata tcggcgtatg tctggggact tccggtcctg cgggcacgga catgatcacc
    46921 gcgctctatt ccgcttctgc tgattccatt cctattctgt gtattaccgg ccaggctccg
    46981 cgcgcccgtc tgcataaaga agatttccag gccgttgata ttgaagcaat tgctaaaccg
    47041 gtcagcaaaa tggcggttac agttcgtgaa gcggcgctgg tgcctcgtgt gctgcaacag
    47101 gcgtttcacc tgatgcgttc tggtcgcccg ggtccggtac tggtggattt accgttcgac
    47161 gtacaggttg cggaaatcga gtttgatcct gatatgtacg aaccgctgcc ggtctacaaa
    47221 cctgctgcca gccgtatgca gatcgaaaaa gctgtagaaa tgttaatcca ggccgaacgt
    47281 ccggtgattg ttgccggagg cggggtaatc aatgctgacg cagctgcact gttacaacag
    47341 tttgctgaac tgaccagcgt tccggtgatc ccaacgctga tgggctgggg ctgtatcccg
    47401 gacgatcatg aactgatggc cgggatggtg ggcctgcaaa ccgcgcatcg ttacggtaat
    47461 gcaacgttgc tggcgtccga catggtgttt ggtatcggta accgttttgc taaccgtcat
    47521 accggttcgg tagagaaata caccgaaggg cgcaaaatcg ttcatatcga tattgagccg
    47581 acgcaaattg gccgcgtgct gtgtccggat ctcggcattg tctctgatgc taaagcggcg
    47641 ctgacgctgc tggttgaagt ggcgcaggag atgcaaaaag cgggtcgtct gccgtgtcgt
    47701 aaagaatggg tcgccgactg ccagcagcgc aaacgcactt tgctgcgcaa aacccatttc
    47761 gacaatgtgc cggtgaaacc gcagcgcgtg tatgaagaga tgaacaaagc ctttggtcgc
    47821 gatgtttgtt atgtcaccac cattggtctg tcacaaatcg ctgcggcaca aatgctgcat
    47881 gtctttaaag accgccactg gatcaactgt gggcaggcag gcccgttggg ctggaccatt
    47941 ccggcggcgc tgggcgtgtg tgccgctgat ccagagcgca atgtggtggc gatttccggt
    48001 gacttcgact tccagttcct gattgaagaa ttagccgttg gcgcacagtt caacattccg
    48061 tacatccatg tactggtcaa taacgcctat ctgggtctga ttcgccagtc gcagcgcgcg
    48121 tttgatatgg attactgcgt acagctcgcg ttcgaaaata ttaactccag cgaagtgaac
    48181 ggttacggcg tcgaccacgt aaaagtagcg gaaggtttag gttgtaaagc aattcgcgtt
    48241 ttcaaaccgg aagatattgc accagccttt gaacaggcga aaatcttaat ggcgcaatat
    48301 cgggtaccgg tagtcgtgga agttattctc gagcgtgtga ccaatatttc gatgggcagt
    48361 gaactggata acgtcatgga atttgaagat atcgccgata acgccgcgga cgcaccgact
    48421 gaaacctgtt tcatgcacta tgaataaggg agataaataa tgttacgttt ctctgctaat
    48481 ttatcgatgt tatttggtga atatgatttt ctcgcccgtt ttgagaaagc tgcgcagtgt
    48541 ggttttcgcg gcgttgaatt tatgttccct tatgactacg acattgaaga attaaaacag
    48601 gtgctggcga gtaataaact cgaacatacg ctgcacaatt taccggcagg tgactgggcg
    48661 gcgggcgagc ggggtattgc ttgtattcct ggccgtgaag aagagtttcg ggatggcgta
    48721 gcagcagcga ttcgttatgc tcgtgcgctg ggtaataaaa aaattaactg tctggtcggt
    48781 aaaacgccga ctggtttcag cagtgaacag attcacgcaa cgcttgtaga aaacctgcgt
    48841 tatgccgcga atatgctgat gaaagaagat atcttattac tgattgaacc tatcaaccat
    48901 tttgatattc ctggtttcca tctcaccgga actcggcagg cgctgaaact gattgatgat
    48961 gttggttgct gcaatttgaa aattcagtat gacatttatc atatgcagcg gatggaaggt
    49021 gaattaacca acactatgac tcaatgggcc gataaaattg gtcacctgca aattgccgat
    49081 aatccgcatc gcggcgaacc gggaaccgga gaaattaatt acgagtatct ctttacggta
    49141 attgaaaatt ctgactacaa cggttgggtt gggtgtgaat ataaacccca aaccaccacg
    49201 gaagccggtt tacgctggat ggatccgtat cgttaaaacg taacgctatt cagacaatgc
    49261 ttttttaggt cgctaagtcg gcaggggatc gtgttgtctg aattcaggaa aagcgaaatt
    49321 taaaagaggt taattatgaa actgggattt attggcttag gcattatggg tacaccgatg
    49381 gccattaatc tggcgcgtgc cggtcatcaa ctgcatgtca caaccattgg tccggttgct
    49441 gatgaattac tgtcactggg tgccgtcagt gttgaaactg cgcgccaggt aacggaagca
    49501 tcggacatca tttttattat ggtgccggat acgccacagg ttgaagaagt tctgtttggt
    49561 gaaaatggtt gtaccaaagc ctcgttgaag ggcaaaacca ttgttgatat gagctccatt
    49621 tccccgatcg aaactaaacg tttcgctcgt caggtgaatg aactgggcgg tgattatctc
    49681 gatgcgcccg tctccggcgg tgaaatcggc gcgcgtgaag ggacgttgtc gattatggtt
    49741 ggcggtgacg aagcggtatt tgaacgtgtt aaactgctgt ttgaactgct cggtaaaaat
    49801 atcaccctcg tgggcggtaa cggcgacggt caaacctgca aagtagcaaa tcagattatc
    49861 gtagcgctca atattgaagc ggtttctgaa gccctgctat ttgcttcaaa agccggagcg
    49921 gatccggtac gtgtgcgcca ggcgctgatg ggcggctttg cttcctcacg tattctggaa
    49981 gttcacggtg agcgcatgat taaacgcacc tttaatccag gttttaaaat tgcgctgcac
    50041 cagaaagatc tcaacctggc a
//
LOCUS       DAZGJT010000026        48101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZGJT010000026 DAZGJT010000000
VERSION     DAZGJT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.026"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..1219)
                     /gene="dnaJ"
                     /locus_tag="ACHKFK_001637"
     CDS             complement(89..1219)
                     /gene="dnaJ"
                     /locus_tag="ACHKFK_001637"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO9051999.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTNSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1308..3224)
                     /gene="dnaK"
                     /locus_tag="ACHKFK_001638"
     CDS             complement(1308..3224)
                     /gene="dnaK"
                     /locus_tag="ACHKFK_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO9052000.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3596..4000
                     /gene="yaaI"
                     /locus_tag="ACHKFK_001639"
     CDS             3596..4000
                     /gene="yaaI"
                     /locus_tag="ACHKFK_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO9052001.1"
                     /translation="MKSVITISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASIYFKATRSASQTLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4026..4739
                     /gene="msyB"
                     /locus_tag="ACHKFK_001640"
     CDS             4026..4739
                     /gene="msyB"
                     /locus_tag="ACHKFK_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO9052002.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPERHHRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEISTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            4888..5454
                     /gene="satP"
                     /locus_tag="ACHKFK_001641"
     CDS             4888..5454
                     /gene="satP"
                     /locus_tag="ACHKFK_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO9052003.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMQKLGLTDAPNAQFLGVY
                     LGLWGIFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            5513..5611
                     /locus_tag="ACHKFK_001642"
                     /pseudo
     CDS             5513..5611
                     /locus_tag="ACHKFK_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5659..6246)
                     /gene="mog"
                     /locus_tag="ACHKFK_001643"
     CDS             complement(5659..6246)
                     /gene="mog"
                     /locus_tag="ACHKFK_001643"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO9052004.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARREVSE"
     gene            complement(6361..7314)
                     /gene="tal"
                     /locus_tag="ACHKFK_001644"
     CDS             complement(6361..7314)
                     /gene="tal"
                     /locus_tag="ACHKFK_001644"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9052005.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAVDQEKLEKMIGDLL"
     gene            7593..9023
                     /gene="yaaJ"
                     /locus_tag="ACHKFK_001645"
     CDS             7593..9023
                     /gene="yaaJ"
                     /locus_tag="ACHKFK_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO9052006.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDANGQFRGRPAWYMARGLGMRWMGVLFAVFLLIAYAIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFAMLAITRGLHGVARLMQGFVPLMAIIWVLT
                     CLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPEIGRQLSPDAWDDVSQE"
     gene            9093..9869
                     /gene="yaaA"
                     /locus_tag="ACHKFK_001646"
     CDS             9093..9869
                     /gene="yaaA"
                     /locus_tag="ACHKFK_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO9052007.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISSLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(9919..10215)
                     /locus_tag="ACHKFK_001647"
     CDS             complement(9919..10215)
                     /locus_tag="ACHKFK_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO9052008.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPYGPPPSPRHNKHNDHRGDHRPGPDKHHR"
     gene            complement(10429..11715)
                     /gene="thrC"
                     /locus_tag="ACHKFK_001648"
     CDS             complement(10429..11715)
                     /gene="thrC"
                     /locus_tag="ACHKFK_001648"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO9052009.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11716..12648)
                     /gene="thrB"
                     /locus_tag="ACHKFK_001649"
     CDS             complement(11716..12648)
                     /gene="thrB"
                     /locus_tag="ACHKFK_001649"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO9052010.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12650..15112)
                     /gene="thrA"
                     /locus_tag="ACHKFK_001650"
     CDS             complement(12650..15112)
                     /gene="thrA"
                     /locus_tag="ACHKFK_001650"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO9052011.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15138..15257)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15193..15258)
                     /gene="thrL"
                     /locus_tag="ACHKFK_001651"
     CDS             complement(15193..15258)
                     /gene="thrL"
                     /locus_tag="ACHKFK_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO9052012.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15472..16158)
                     /gene="yjtD"
                     /locus_tag="ACHKFK_001652"
     CDS             complement(15472..16158)
                     /gene="yjtD"
                     /locus_tag="ACHKFK_001652"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9052013.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPVA
                     RWVAHGSGDIIDNIKVFKTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERAMTLLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16558..16698)
                     /gene="yjjY"
                     /locus_tag="ACHKFK_001653"
     CDS             complement(16558..16698)
                     /gene="yjjY"
                     /locus_tag="ACHKFK_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO9052014.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AI"
     gene            16794..17510
                     /gene="arcA"
                     /locus_tag="ACHKFK_001654"
     CDS             16794..17510
                     /gene="arcA"
                     /locus_tag="ACHKFK_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO9052015.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17570..18922)
                     /gene="creD"
                     /locus_tag="ACHKFK_001655"
     CDS             complement(17570..18922)
                     /gene="creD"
                     /locus_tag="ACHKFK_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO9052016.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDV
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNVALNLSGTGDLSVVPGGRYS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(18980..20404)
                     /gene="creC"
                     /locus_tag="ACHKFK_001656"
     CDS             complement(18980..20404)
                     /gene="creC"
                     /locus_tag="ACHKFK_001656"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO9052017.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILCEGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGCITLS
                     AEVDQEYVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20404..21093)
                     /gene="creB"
                     /locus_tag="ACHKFK_001657"
     CDS             complement(20404..21093)
                     /gene="creB"
                     /locus_tag="ACHKFK_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO9052018.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFDVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARNEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21106..21579)
                     /gene="creA"
                     /locus_tag="ACHKFK_001658"
     CDS             complement(21106..21579)
                     /gene="creA"
                     /locus_tag="ACHKFK_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO9052019.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            21790..22659
                     /gene="robA"
                     /locus_tag="ACHKFK_001659"
     CDS             21790..22659
                     /gene="robA"
                     /locus_tag="ACHKFK_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO9052020.1"
                     /translation="MDQAGIIRDLLIWLEGHLDHPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNSPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(22656..23303)
                     /gene="gpmB"
                     /locus_tag="ACHKFK_001660"
     CDS             complement(22656..23303)
                     /gene="gpmB"
                     /locus_tag="ACHKFK_001660"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO9052021.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLETRNIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            23355..23882
                     /gene="yjjX"
                     /locus_tag="ACHKFK_001661"
     CDS             23355..23882
                     /gene="yjjX"
                     /locus_tag="ACHKFK_001661"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO9052022.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEVLGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRTSVYHQAVIL
                     ALSPFHNAVYQPLQA"
     gene            complement(23955..24281)
                     /gene="trpR"
                     /locus_tag="ACHKFK_001662"
     CDS             complement(23955..24281)
                     /gene="trpR"
                     /locus_tag="ACHKFK_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO9052023.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLED
                     VLLKSD"
     gene            complement(24371..26308)
                     /gene="sltY"
                     /locus_tag="ACHKFK_001663"
     CDS             complement(24371..26308)
                     /gene="sltY"
                     /locus_tag="ACHKFK_001663"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO9052024.1"
                     /translation="MEKAKHVTWRLLAVGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVSANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            26515..28182
                     /gene="ettA"
                     /locus_tag="ACHKFK_001664"
     CDS             26515..28182
                     /gene="ettA"
                     /locus_tag="ACHKFK_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO9052025.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(28238..28522)
                     /gene="nadS"
                     /locus_tag="ACHKFK_001665"
     CDS             complement(28238..28522)
                     /gene="nadS"
                     /locus_tag="ACHKFK_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9052026.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKEPARVTRYEIADVKAIREQLNV
                     SQSEMAKALGTSVDTIKSWESKRRNPTGLAAKVLATIKENPAFFRELASH"
     gene            complement(28524..28856)
                     /locus_tag="ACHKFK_001666"
     CDS             complement(28524..28856)
                     /locus_tag="ACHKFK_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9052027.1"
                     /translation="MGKAIIFIETPMFTRQIKQIATDDELKELQKELIGTPDKGDLIQ
                     QTGGLRKVRMAAGSQGKSGSVRIIYFLATEEIIYLIMAYPKNAKDSLTDTEKAQLKKL
                     TKLLKGEI"
     gene            complement(28948..30180)
                     /gene="nadR"
                     /locus_tag="ACHKFK_001667"
     CDS             complement(28948..30180)
                     /gene="nadR"
                     /locus_tag="ACHKFK_001667"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO9052028.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRYLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(30201..31583)
                     /gene="radA"
                     /locus_tag="ACHKFK_001668"
     CDS             complement(30201..31583)
                     /gene="radA"
                     /locus_tag="ACHKFK_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO9052029.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDYLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(31632..32600)
                     /gene="serB"
                     /locus_tag="ACHKFK_001669"
     CDS             complement(31632..32600)
                     /gene="serB"
                     /locus_tag="ACHKFK_001669"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO9052030.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGERVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKTEVTIRHADLMGVFCILSGSLNQK"
     gene            32706..33350
                     /gene="ytjB"
                     /locus_tag="ACHKFK_001670"
     CDS             32706..33350
                     /gene="ytjB"
                     /locus_tag="ACHKFK_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO9052031.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVDVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            33378..34394
                     /gene="lplA"
                     /locus_tag="ACHKFK_001671"
     CDS             33378..34394
                     /gene="lplA"
                     /locus_tag="ACHKFK_001671"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO9052032.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNSDLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITEAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFTWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPEQEKELRELSAWMA
                     GAVR"
     gene            34426..34689
                     /locus_tag="ACHKFK_001672"
     CDS             34426..34689
                     /locus_tag="ACHKFK_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9052033.1"
                     /translation="MIDLENQEREIINLMFSQDISWLTAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAGIYGCPPELLICPSWMTAEHK"
     gene            complement(34850..35569)
                     /gene="deoD"
                     /locus_tag="ACHKFK_001673"
     CDS             complement(34850..35569)
                     /gene="deoD"
                     /locus_tag="ACHKFK_001673"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO9052034.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(35626..36849)
                     /gene="deoB"
                     /locus_tag="ACHKFK_001674"
     CDS             complement(35626..36849)
                     /gene="deoB"
                     /locus_tag="ACHKFK_001674"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO9052035.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPSYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(36901..38223)
                     /gene="deoA"
                     /locus_tag="ACHKFK_001675"
     CDS             complement(36901..38223)
                     /gene="deoA"
                     /locus_tag="ACHKFK_001675"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO9052036.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(38301..39080)
                     /gene="deoC"
                     /locus_tag="ACHKFK_001676"
     CDS             complement(38301..39080)
                     /gene="deoC"
                     /locus_tag="ACHKFK_001676"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO9052037.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIDIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKKACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            39338..40888
                     /gene="yjjI"
                     /locus_tag="ACHKFK_001677"
     CDS             39338..40888
                     /gene="yjjI"
                     /locus_tag="ACHKFK_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO9052038.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPAL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAHGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPDITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLEDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            40860..41723
                     /gene="yjjW"
                     /locus_tag="ACHKFK_001678"
     CDS             40860..41723
                     /gene="yjjW"
                     /locus_tag="ACHKFK_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO9052039.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVAWSAAACEQCDTCLKMCPQHATPMVQSMSVDEV
                     LNHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLCHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAALIKGLGDVPVRLNAFHAHGVYGEAQSWPSATPEDVEQLADALRERG
                     VSRLIFPALYL"
     gene            complement(41940..42719)
                     /gene="yjjV"
                     /locus_tag="ACHKFK_001679"
     CDS             complement(41940..42719)
                     /gene="yjjV"
                     /locus_tag="ACHKFK_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026291.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9052040.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERHPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYTLFNVP"
     gene            complement(42716..43789)
                     /gene="yjjU"
                     /locus_tag="ACHKFK_001680"
     CDS             complement(42716..43789)
                     /gene="yjjU"
                     /locus_tag="ACHKFK_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO9052041.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYREIQQFIEKPPGQLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLSRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(43911..44072)
                     /gene="ytjA"
                     /locus_tag="ACHKFK_001681"
     CDS             complement(43911..44072)
                     /gene="ytjA"
                     /locus_tag="ACHKFK_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO9052042.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(44199..44804)
                     /gene="osmY"
                     /locus_tag="ACHKFK_001682"
     CDS             complement(44199..44804)
                     /gene="osmY"
                     /locus_tag="ACHKFK_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO9052043.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(45197..46783)
                     /gene="prfC"
                     /locus_tag="ACHKFK_001683"
     CDS             complement(45197..46783)
                     /gene="prfC"
                     /locus_tag="ACHKFK_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO9052044.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVA
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            47003..47251
                     /locus_tag="ACHKFK_001684"
     CDS             47003..47251
                     /locus_tag="ACHKFK_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9052045.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKLYDDVDVIVKS
                     ASNDGLTILRSVDKESAQKFVQETLQEVWETADDWFIR"
     gene            complement(47288..47461)
                     /locus_tag="ACHKFK_001685"
     CDS             complement(47288..47461)
                     /locus_tag="ACHKFK_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052046.1"
                     /translation="MLPDLQRLQVIQGQMMNKDKCRMYGRKRYTAGAMCFMLFVWLGG
                     VVTLCAVAAIIMQ"
     gene            complement(47466..47939)
                     /locus_tag="ACHKFK_001686"
     CDS             complement(47466..47939)
                     /locus_tag="ACHKFK_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052047.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVILAREFFLPENRRYIPDFD
                     ESWLIICDTLLGRLMRCMFQGRHFLQLGAESLRDGEQISDAIRNGVWTYNSVARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVKHLACAE"
BASE COUNT        11402 a        13020 c        11930 g        11749 t
ORIGIN      
        1 tgatccccgc cactttacag gtgctcgcat atcttcaacg caccctggtt tttaccctgg
       61 cctgcccacg ggcaggcttt tggggaggtt agcgagtcag gtcgtcaaaa aacttcttca
      121 cgccatcaaa gaagctcttt gaacgcgggc tgttgtgctc gccggttggg ccaccaaagc
      181 tttcttgcag ctcttgcagc agctgtttct gcttctcgtt caaacctacc ggtgtttcga
      241 caacaacgcg gcatagcaaa tcaccctgtg cgccaccgcg gacagacttg acgcctttac
      301 cgcgcatacg gaacagctta ccggtctggg tttcgccagg cactttcagt ttgacgcgac
      361 catcaagggt cggtacttcg atttcaccac ccagcgccgc catagcgaag ttgatcggga
      421 cttcgcaata caggttgttg ccttcacgct cgaaaatcgg gtgctgttta acctgaacct
      481 gaacgtacag atcgcctgcc ggtgcgccgt gttcacccgc ttcaccttcg cccgcaagac
      541 ggatgcggtc tccagtgtcc acccctgccg ggattttaac ggacagcgtt ttgctgcgct
      601 caacacgacc atgaccatga catttgttgc acggatcttt gatcagcgta ccgcggccct
      661 gacagtgtgg acaggtctgc tgcacggcaa agaaaccctg gcgcatctgc acctggccag
      721 aaccatgaca ggtcggacag gtctgcggct gtgtacctgg ttttgcaccg ctaccgtggc
      781 aaacgtcaca ctcttccaga gtcggaatgc ggatctcttt ggtcacgcca cgtacagctt
      841 cttcgagggt gagctccatg ttatagcgta aatcagcacc gcgcgccgca cgttgacgac
      901 cacgtccgcc gccaaaaata tcgccgaaaa cgtcaccaaa aatatcgctg aagtctgcgc
      961 cgccgccaaa accgccgccg cccatgccgc cttgctcaaa cgcagcatga ccatactgat
     1021 cgtatgccgc acgtttttgc gagttggtca gaacttcata agcttccttg atctctttaa
     1081 atttcgcctc ggcctctttg tcaccctggt tacggtccgg gtggtatttc atggccaggc
     1141 gtttgtaggc ctttttgatt tcacgctctt ccgctgtttt ggaaacgcct aaaatctcgt
     1201 aataatcttg cttagccatc ttttttgatt tgcccctaga tgaatgcacg ggcggagagg
     1261 aaattcccct tcgcccgtgt cagtataatt acccgtttat agggcgatta ttttttgtct
     1321 ttgacttctt caaattcagc gtcgacaaca tcgtcatctt tcgcgttgtt tgcagaagca
     1381 tcagcaccgg cagtctgctg ctgggcatgt tgctgctggg cgatttccat cagtttctgg
     1441 gaaacctgtg ccagttcctg cattttcgct tcgatagcgg ctttgtcttc acctttcaga
     1501 gcagtttcca gtgcagtcag cgcagactcg atagcagttt tgtcgtcagc cggcagtttg
     1561 tcgcctgctt cttcaacctg cttacgggtg ctgtgcagca gatggtcgcc ctggttgcga
     1621 gtctgtacca gctcttcaaa cttacggtca gcttcggcgt tagcttctgc gtcgcgtacc
     1681 attttctgga tttcatcttc gttcagacca gaagaagcct tgatagtgat cttctgctct
     1741 ttaccgctgt ttttgtcttt cgcggaaacg tgcaggatac cgtcagcatc gatatcgaag
     1801 gtaacttcga tctgcggcat gccgcgcggt gccgggttga taccatccag gttgaactga
     1861 cccagagatt tgttatcagc cgcacgttta cgttcaccct gcagcacatg gatggttacc
     1921 gcagactggt tgtcttcagc ggtagagaac acctggctgt gcttggtcgg gatagtggtg
     1981 tttttcgcga tcagcgtggt catcacaccg cccatggttt cgatacccag agacagcggg
     2041 gtaacgtcca gcagcagtac gtcttttacg tcaccagtca gaacaccacc ctgaacagca
     2101 gcaccgatgg ctacagcttc gtccgggtta acatctttac gcggctcttt accaaagaat
     2161 tcagcaactt tcttctgaac cattggcata cgagtctgac caccaacgag gataacgtcg
     2221 tcgatatcag atacggacag gccagcgtcc tgcagtgcaa ctttcagcgg ctcaatggaa
     2281 cggtttacca gatcttcaac caagctttcc agtttcgcac gagtcacttt gatgttcatg
     2341 tgtttcggac cggtcgcgtc tgcagtgatg tacggcaggt taacgtcggt ctgctgagcg
     2401 gaagacagtt cgattttcgc tttttctgcc gcttctttca ggcgctgcat tgccagcgga
     2461 tcgttgcgca ggtcaatgcc ctgatctttc ttgaattctt caaccagata gttgatcaga
     2521 cgactgtcga agtcttcacc acccaggtgg gtatcaccgt tggttgccag aacttcgaag
     2581 gttttttcgc cgtcaacttc gtcgatttcg ataatggaaa tatcgaaagt accaccaccc
     2641 aggtcataaa ccgcgatagt acggttgcca gtacctttgt ccagaccgta agccagcgca
     2701 gctgcggtcg gttcgttgat gatacgtttt acttccagac cagcgatacg gcctgcgtct
     2761 ttggttgcct gacgctgagc atcgttaaag tatgccggta cggtaataac agcttcagtt
     2821 accggttccc ccaggtaatc ttcagcggtt ttcttcattt ttttcagcac ttcagcagag
     2881 atctgcggcg gtgccatttt ctggccttta acttcgaccc atgcgtcgcc gttatcagca
     2941 gcaataattt tgaacggcat gatggaaaca tcacgctgta cttcttcgtc ctggaagcgg
     3001 cggccaatca ggcgtttaat cgcaaacagg gtgttttgcg ggttcgtcac tgcctgacgt
     3061 ttagccggct gaccaaccag agtttcacca tcctgggtat aggcaatgat agaaggcgtg
     3121 gtgcgatcgc cttcggcgtt ctccagtaca cgaggagtgg tgccatccat aatcgctaca
     3181 caagagttgg tagtacccag gtcgatacca attattttac ccatctaaac gtctccacta
     3241 tatattcggt catcatgtgg ttgtgagtct gtaatagggg cgaaacgtct ggtttcaact
     3301 gcccaatttc atttttttgc agactcactc actgcggttg actactaaat ggggtcgtaa
     3361 cccacgtcat caagggggag atgcaaaaat tttttgtgct tttgcgcaga aatttcgcga
     3421 tatggcagga caagacgtga aactgacaac aattttattc atataaagaa tgaatcgcag
     3481 ccaaggcatt catcaaaaaa ttgtaataaa aagaaaatat tacgtacctg aaacttctct
     3541 ttatcagcag taaactagtt tcatcccctt acccctcccc accaagagaa tccttatgaa
     3601 atccgttatt acgatttccg ccagcctggc gattagcctg atgctgtgct gcacggcgca
     3661 ggcaaacgac cataaaatcc tcggcgtcat tgcaatgccg cgaaacgaaa ccaacgatct
     3721 ggcgctgaaa cttcctgttt gtcgcattgt taaacgcata caactctcag ccgaccatgg
     3781 cgatttgcag ttaagcggtg catcgattta tttcaaagcc acccgaagcg ccagtcagac
     3841 cctgaatatt ccttcagaaa taaaagaagg gcaaaccact gactggatca acattaacag
     3901 cgataacgac aacaaacgct gcgtctcaaa aatcaccttt tccggtcata cagtgaactc
     3961 atcggatatg gccacgctga aaattatcgg cgacgactaa cgccagaata tagaaaccac
     4021 aaaaaatgaa tgttaattac ctgaatgatt cagatctgga ttttctccag cattgtagtg
     4081 aggaacagtt ggcaaatttc gcccggctgc tcacccataa tgaaaaaggc aaaactcgcc
     4141 tctccagcgt actgatgcgc aacgaactgt ttaaatcgat ggaaggacat cccgagcgac
     4201 accaccgcaa ctggcagctg attgccggag aattacagca ttttggtggc gatagtatcg
     4261 ccaacaaact gcgcggacac ggtaaattgt atcgcgccat tttgctcgat gtttcaaagc
     4321 gattgaagct gaaagccgac aaagagatat ctacgtttga aattgagcaa cagttactgg
     4381 aacaatttct gcgtaatacc tggaagaaaa tggacgagga acataagcag gagtttctgc
     4441 acgcggtcga tgccagggtg aatgagctgg aagagctgct gccgctgctc atgaaagaca
     4501 aattattggc aaaaggtgtg tcgcatttgc tttccagcca attgacccgc attttacgca
     4561 cccacgcagc aatgagcgtg cttgggcatg gtttgctgcg cggcgcaggt ctgggaggcc
     4621 ctgtgggcgc agcattaaat ggggttaaag cggtcagcgg cagtgcctat cgcgtgacaa
     4681 ttccagccgt actgcaaatc gcctgcctgc gccggatggt tagcgccact caggtctgat
     4741 accggaaacc ggggaaaatt ttcattttcc cctcaaaaga tcgtagacac tgccccactg
     4801 gctgattatt atgccgcgcc ctgaaaacac tacagttatt cagggaaatt atttcaccat
     4861 tcattcgatg atgatttttg aggaattatg ggcaacacta agttggctaa tccggcaccg
     4921 ctgggcctga tgggcttcgg catgaccacc attctgctta acctgcacaa cgtgggttat
     4981 tttgctctgg acggtattat tcttgccatg ggcattttct acggcggcat cgcgcaaatt
     5041 tttgccggtc tgctggagta caaaaaaggc aacactttcg gtttaaccgc attcacctct
     5101 tacggttctt tctggctgac gctggttgca attctgctga tgcagaaact ggggctgacc
     5161 gatgcgccaa atgcacagtt ccttggtgtc tacctgggtc tgtggggcat atttacgctg
     5221 tttatgttct tcggcacgct gaaaggcgca cgcgttctgc aattcgtttt ctttagcctg
     5281 accgtgctgt ttgccctgct ggcgatcggt aacattgccg gtaacgccgc gatcatccac
     5341 tttgccggct ggattggtct tatctgcggt gccagcgcaa tctatctggc gatgggtgaa
     5401 gtgctgaacg agcagtttgg tcgcaccgtt ctgccgattg gtgagtctca ctaattgtta
     5461 agctcccccc gctcgaaggg ataagattgt taacaacgtg cgctttgttt atgccggatg
     5521 cggcgtgaac gccttatccg gcctacaaaa tcgtgcaaat tcaatatatt gcaagaagat
     5581 cgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgtcatcag cctgatcccc
     5641 ccgcctgggg ggattttttt attcgctaac ttcgcgtctt gcactcttcg gtctgaatgc
     5701 tgcaaccact tccggtgccg tttcaacgta tggcccttcc agcaactgaa tgcagtacgg
     5761 tacgctggca aaaataccgt gcaccacaac gttaccctca gcgtccttca caccttccag
     5821 cgtctcttta atagacttcg gttgaccggg taagttaagg atcagcgcct gtttgcgaat
     5881 cacccccacc tgacgcgaaa ggatcgcagt tggtacaaaa tgcaggctga tctggcgcat
     5941 ctgttcacca aagcctggca tctcgcggtc cgctactgcc agcgtcgcat cgggcgttac
     6001 gtcacgacgc gccgggccag ttccgcccgt ggtgagcacc agatggcaac tcatttcatc
     6061 caccagctca cacaacgttt gctcgatgat cgcctgctca tcggggatta agcgggtttc
     6121 cagttcaaac ggcgtggtta gcgccgatgt cagccattct tccagcgcag ggatgccttt
     6181 atcctgataa acgccgctgg atgcgcgatc agagatggaa actaagccaa tacgtaaagt
     6241 attcatcaag atttttccgt ttaaacagtc tcgttaaaca gaatgataca ctgcgaaggg
     6301 agtgacagac aggtttcaga agaggtagcg tgaccgactt cccggtcacg ctaagtatga
     6361 ttacagcaga tcgccgatca ttttttccag tttttcctgg tcaacagcaa acttacggat
     6421 accttccgcc agtttatcta ctgccattgg atcctggttg tgctgccaca ggaactcgga
     6481 ctcagtgata cgcgccggac gcgctttcac ttcaccagtg taagacagtt tacgttcgat
     6541 agccccttcg ctctccgcca gctctttcag cagtgccggt gcgatggtca gacggtcgca
     6601 gcctgccagt tccagaattt cgccgatgtt acggaagctt gcgcccataa ccacggtttc
     6661 ataaccatgc tctttgtagt actggtagat ttcagataca gaaaccacgc ccggatcttc
     6721 tgccggagcg tactctttct tatcggtatt cgctttgtac cagtcaagaa tacggccaac
     6781 aaacggcgag atcaggaaca cgcccgcttc cgcacaagca cgagcctgag cgaaggagaa
     6841 cagcagggtc aggttacagt tgatgccttc tttttccagc tgttccgcag cacggatacc
     6901 ctgccaggta gaagccagtt tgatcagaat acgatcgttg ctaatacctg catcgttgta
     6961 gagtttgatc aggcgttttg ctttcgcaat tgacgcttcg gtgtcatagg aaagacgcgc
     7021 atcaacttca gttgagatac ggcccggaac cagtttcagg atttccagac caatatttac
     7081 ggccagtttg tcggtcgcgt ccacgatctg ctgcgcgcga tcgttgctct gctgtttcgc
     7141 ccaggcgaca gcatcatcaa tcaacttacg gtattccgga atctgcgctg cgttaagaat
     7201 gagagaaggg ttggttgtgg catcctgcgg ttgatacagc ttcattgccg cgatgtcccc
     7261 agtgtcggcc actacggtgg tgtactgacg aagggaggtc aatttgtccg tcatgatagt
     7321 atttctcttt aaacagcttg ttagggggat gtaaccggtc tgccctgatg atatcacgac
     7381 gcaacgtcag cgcaaccgcc gacaagacgg ttatcgccag gcgagactgt ttcggatttc
     7441 tgacactgcg ttgatatcgc ccgccatttt tatacaaaac ctcatgtatg ctacgcagaa
     7501 gttatcaagt acctcgtagc gtatatactt cttaaacaat tggtaacgtt tacacaggaa
     7561 agtcatcgcg accggcaata agagggatat gcatgccaga ttttttctcc tttattaaca
     7621 gcgtcctttg gggatcggta atgatttacc tgctcttcgg cgcaggttgt tggttcactt
     7681 ttcgcaccgg atttgtgcag tttcgctaca tccgacagtt tggcaaaagt cttaaaaata
     7741 gcattcatcc acagccaggc ggtttaacct catttcagtc attgtgtacc agtcttgcag
     7801 cgcgcgtggg tagcggcaac ctggccggcg ttgcgctggc aattaccgcc ggtggacctg
     7861 gagccgtctt ctggatgtgg gttgccgcat ttatcggcat ggcgacctcg tttgccgaat
     7921 gttccctcgc acagctttat aaagaacgtg atgccaatgg gcagtttcgt ggcagaccgg
     7981 catggtatat ggcgcgcggg ctggggatgc gctggatggg cgttctgttc gccgtctttt
     8041 tgctcatcgc ctacgccata attttcagcg gagttcaggc gaacgccgtt gcgcgagccc
     8101 tgagtttttc ttttgatttt cccccgctgg tgacaggtat tattctcgct gtctttgcta
     8161 tgctggcgat cactcgcggt cttcatggcg tcgcccggct catgcagggg tttgtcccgt
     8221 tgatggcgat aatctgggta ctgacctgcc tggtgatttg cgtaatgaat atcgggcaac
     8281 ttccccacgt catttggtct atttttgaga gtgcttttgg ctggcaggaa gctgcaggcg
     8341 gcgcggcggg atatacctta agccaggcga ttactaacgg ttttcagcgc agtatgtttt
     8401 ccaatgaggc gggaatgggg tcgacgccaa acgcggcagc ggcagctgcg tcctggcctc
     8461 cgcatccggc agcgcaagga attgtccaga tgattggcat ttttatcgac accctggtca
     8521 tctgcacggc aagcgccatg ctgatattac tggcgggtaa cggcacaacc tacatgccgc
     8581 tggaaggtat tcagcttatc cagaaggcga tgcgggtgct aatgggttcc tggggtgcgg
     8641 agtttgttac cctcgtggtt attctgtttg ccttcagctc catcgttgcc aactacattt
     8701 atgccgaaaa caatctcttc tttttacgcc tgaacaaccc taaagcgatc tggtgtctgc
     8761 ggatctgcac cttcgcaacg gtcatcggcg gcaccttgct aagtcttccg ctgatgtggc
     8821 aactggcaga tatcattatg gcctgcatgg ctattaccaa tttgaccgcc atattactgc
     8881 tctcgcctgt ggttcatacc attgccagtg attatctacg ccagcgtaaa ctcggcgtgc
     8941 gcccggtgtt tgatccgttg cgttatccgg agatcggtcg ccagctttct ccagacgcgt
     9001 gggatgatgt ttcgcaggag taatcacaac tatcgatcaa ctcattctct ttttttgcta
     9061 aagtcggcat aaatttcctg caaggactgg atatgctgat tcttatttca cctgcgaaaa
     9121 cgcttgatta ccaaagcccg ttgaccacca cgcgctatac gctgccggag ctgttagaca
     9181 actcccagca gctgatccat gaagcgcgga aactgacgcc cccgcagatt agctccctga
     9241 tgcgcatcag cgacaaactg gcgggtatca acgccgctcg ctttcatgac tggcagccag
     9301 atttcacacc ggagaatgcc cgccaggcaa ttctggcgtt taaaggtgat gtctacactg
     9361 gcttgcaggc cgaaaccttc agcgaagacg atttcgattt tgcccaacag catttgcgaa
     9421 tgctttccgg cttgtatggc gtactgcgcc cgctcgattt aatgcagcct tatcgtctgg
     9481 aaatggggat acgtcttgag aatgcccgag ggaaagatct gtatcaattc tggggagata
     9541 tcatcaccaa caagctgaac gaggcgctcg cagcacaagg cgataatgta gtgataaatc
     9601 tggcgtcaga tgagtatttt aaatcggtga agccgaagaa attgaatgcc gagattatca
     9661 aaccagtgtt cctcgatgag aagaacggca aatttaagat aatcagcttc tacgctaaga
     9721 aagcacgcgg tctgatgagt cgtttcatta ttgaaaatcg gctgaccaaa ccagagcaac
     9781 tgactggttt taatagcgaa ggttacttct ttgatgaagc ttcctccagc aatggcgaac
     9841 tggtgtttaa acgctacgaa caacgttaag tttgtagtca atgccggata atgccggatg
     9901 gcaatccggc attgatattt agcgatgatg tttgtcaggc cccggacgat gatcgccacg
     9961 atgatcattg tgcttgttat ggcgcggcga tggcggcggt ccatatgggt gccagcgatt
    10021 gcctcgccat tcataatgtt gtttccacca gccgtggtcg cgccagtgac cgccatccca
    10081 gtaatagcca cgattatcac gatcgcctat ctgtaatttt actgacggga ctagcgtaat
    10141 ttccgcagcc tgcgctgcca tgggagcgac cagaaccagg gaaagtgcga gtacgataga
    10201 ttgcatcttt ttcacaggtt actcctttta tcattgcccg ttatgggtcg atatccggag
    10261 attacgcgat gagaacggca gttgttattc tccgcaatcc taatctctaa gttaccgcat
    10321 ttattgagaa tttctccttt ttccctgtcg tttttctcca tattttcttc ataaaaaagc
    10381 cgggctgcat aaaagcaaac ccggcctgat tgagataatg aatagacgtt actgatgatt
    10441 catcatcaat ttacgcaacg cagcaaaatc ggcgggcagg ttatgcgaaa gcaagggtaa
    10501 atcagcacgt tctgccagct cttttggcag atccaacgtt tcaccgagaa tcgcttccac
    10561 gctctcttta aatttcgccg gatgcgcggt gccgaggaac aagccatatt cgcctggatt
    10621 caactggtca cgcagcgcac gataagctac ggcagcgtgc ggctccgagg tatagcccag
    10681 ttcttttaac tcacgcattg tctgttgcgt ggtttcatca tccacggctg cataccccag
    10741 ctctttcagt tgccagattt tgcggcggaa caactcttcc acacgcggcc agttgtttgg
    10801 ctggctaaca tccatcgcat tggataacgt cgcctgagtc gctttgggtg accactgacc
    10861 gtcgtgcagg aaacgtggta cggtatcgtt cacgttggtc gcagcaataa aacgttttac
    10921 cggcagaccg agtgacttcg ccagcagacc tgccgttaaa tcaccgaagt ttccactcgg
    10981 taccgagaca accaactggt tacgtgcttc ttgcggcaac tgtgcgacag cctcaaagta
    11041 ataacaaatc tgcgccagca agcgactgat gttgatggag ttagcagaat tcagccccag
    11101 cgccactttc agttcttcat catcaaacgc ctgcttcacc agcgcctgac aggcatcgaa
    11161 atcgccgtcg atggcaacag tttcgatatt gccgcccaat gtacagaaca gtttttcttg
    11221 cagtggactg attttgcctc gtggatagag gataaccact ttcacattcg gtaaaccgta
    11281 gaaagcatga gccactgccg ctccagtatc accagatgtc gcggtcagaa tggtcactgg
    11341 cttatcgccc gcaatatggg tcagcatttg tgccataaag cgaccgccga aatctttaaa
    11401 tgccagcgtt ggcccgtgaa acaactccag acaaccgaca tcgctttcaa cattggcgac
    11461 cggagccggg aaggcaaacg ccgcgcgtac gcgctcttcc aggatttcct gcgggatttc
    11521 atcaccaata aacgccgaga ggatcttcgc actgcgggtg acaaaatcca gcttcagcat
    11581 ctcatcaatt tcagtcaggc tgaattccgg caggtcgtgc gggaaaaaca gcccctgatt
    11641 tttgcccaac ccctgggtta cggcttgcgc aaagctgacc tgctcattgt gatctttcag
    11701 attgtagagt ttcatttagt tttccagtac tcgtgcgccc gccgtatcca gccggcaaat
    11761 atgaacaaaa ccttcctgat tttgcaggta gttcttaccc aaccagtcgg caacgcgctg
    11821 ggcggtatcc ggcttgtcac acagagcgaa caaagtcggg ccggagccgg agataccgct
    11881 cgctaccgcg ccgatttccg caaccgcctg ccgcgcctgc cggaagcctg gcagtaaccg
    11941 ttcacggtag ggttcagcga taacatcttt catcagcttc gcggcaagct caggctgacg
    12001 ggaatagcag gcgtgaatga agcctgccag atgtcgcccg tgcgcaatgc aatcctggcg
    12061 gcgatactgc gccggtaaaa tagccctggc ttctgccgtc gagactttaa tccccggata
    12121 cgccagcacc cacagccact catcaaaccc tggcacttgc tgactgatga tgtcgttttc
    12181 ttcaatcatc aactgcatac caccaagaaa acacggtgcc acgttgtcgt aatgaatgct
    12241 gccggagata cgcccttcca actcgcccat caaagccagc aaacgagtgt cattaagcgg
    12301 cttgccgcag tgttcattca tcgccatcag cgccgcgacc actgaacagg cgctggagcc
    12361 taagcccgaa ccgatcggca tattcttttc cagagtcatc gccactggaa tttgcttgcc
    12421 aagctcctgg caaaaacgct cccagcactg ataaacgata ttttcccgtg gctctgacgg
    12481 cagcttatcg gcaaagcgtc cgaggttgtt gagactgaat gtctctgccg cctcaaccgt
    12541 gactacatct ccgagcaatg caccatcaac aggtgtcacc gccgccccga gcacatcaaa
    12601 cccgacgctc atattggcac tggaagccgg ggcataaact ttaaccatgt cagactccta
    12661 acttccatga gagggtacgt agcagatcgg caaagacacc ggcagctgta acgtcattgc
    12721 ccgcaccata tccgcgcagt accaacggca gcggctgata atagtggcta tagaaggcca
    12781 gggcgttttc gccatttttc actttgaaca gcggatcatt accatccact tcggcaatct
    12841 tcacgcggca gacgccatct tcatcaatat tgccaacata gcgcaaaact tttccttcat
    12901 cacgggcctt cgccacacgc gcggcaaaga gatcgtcgag ctgtgacaga ttcgccataa
    12961 aagcggcgac atcaccctcg gcgttaaact ctgcgggcag cacaggttca atttcaatat
    13021 ccgccagctc cagttcacgt cccgtttcac gagcgagaat caatagctta cgcgccacat
    13081 ccataccaga aagatcatct cgcgggtccg gttcggtata acccatttcc cgcgccagtg
    13141 tggtcgcctc ggagaaactc atgccttcgt ctaacttgcc gaagatataa gaaagcgaac
    13201 ctgaaagaat gccggagaac ttcaccaatt catcacctgc atttaacaga ttttgcaggt
    13261 tctcaataac cggtaatcca gccccaacgt tggtgtcata gaggaattta cgccgcgatt
    13321 tttccgccgc atgacgcaac agatggtagt aatccatcga cgaggtgtta gcctttttgt
    13381 tcggcgtgac gacgtggaaa ccttcgcgca ggaagtcagc atactgatcc gccactgcct
    13441 ggctggaagt gcagtcaaca atgaccgggt tcagcagatg atattctttc acgaggcgaa
    13501 ttaagcgccc gagattaaac ggctctttgg cttgcgccag ttcttcctgc cagttttcca
    13561 gattaaggcc atgtacattg gtgagcagag ccttcgagtt ggcaacaccg cagacacgta
    13621 agtcgatatg tttattcttc agccagcttt gctgacgctt cagttgctcc agcagcgcac
    13681 cgccaacgcc accgacgcca atcacaaaca cttcgataac ctgatcggta ttgaacagca
    13741 tctgatgagt aacgcgcacg ccagtggtcg catcatcgtt atttaccacg acagagattg
    13801 agcgttcaga agatccctga gcaatggcga caatgttgat attggcgcgg gccagtgcgg
    13861 caaagaattt cgccgagatc ccacgcaagg tgcgcatacc atcacctacc accgagataa
    13921 tggccagccg ttccgtcact gccagcggct ccagtaagcc ctctttcagt tccaggtaga
    13981 actcttcctg cattgcccgt tcagctcgca cacagtcgct ttgtggaacg cagaaactga
    14041 tgctgtattc ggaagatgat tgcgtaatca gcaccacgga aatacgggcg cgtgacatcg
    14101 ctgcaaagac gcgcgccgcc atgccaacca tccctttcat ccccgggccg gaaacgctga
    14161 acattgccat gttattcaga ttggaaatgc ccttgaccgg taattcgtct tcatcacggc
    14221 tggcaccaat gagcgtacct ggtgcttgag gatttccggt atttttaatc aggcaaggga
    14281 tctggaactg ggcgatgggg gtaatggtgc gggggtgaag aactttagcg ccgaaatagg
    14341 aaagctccat cgcttcctgg taggacatcg acttcaacaa cctcgcatcg ggcacctgac
    14401 gcgggtcgca ggtatagacc ccgtcaacgt ccgtccaaat ctcgcaacaa tcggcgcgta
    14461 aacaggcagc cagcaccgcc gcggagtagt cggaaccgtt gcgtccaagc accaccagtt
    14521 cgcctttttc attaccggcg gtgaaacctg ccatcagcac catgtgatca gccggaatac
    14581 gacttgccgc aatacggcga gtggactcag caatatcgac ggtagattcg aggtaatgcc
    14641 ccactgccag cagtttttcg accggatcga taacggtaac gttgtgacca cgcgcttcta
    14701 acacgccggc cataatggcg atcgacattt tctcgccacg gcaaatcagc gcagcgttga
    14761 tgctatccgg gcactgcccc aacaaactga tgccatgcag gacatgtttt atttgggcaa
    14821 attcctggtc gacgaaagtt ttcaattgtg ccagcggaaa tcccggctgg gcggcggcga
    14881 gtcccgtcag aagttcggca aaaatacgtt cggcatcgct gatattgggt aaagcatcct
    14941 ggccgctaat ggttttttca atcatcgcta ccagatggtt ggtgattttg gcgggggcag
    15001 agaggacggt ggccacctgc ccctgcctgg cattgctttc cagaatatcg gcaacccgca
    15061 gaaaacgttc tgcatttgcc actgatgtac cgccgaactt caacactcgc atggttgtta
    15121 cctcgttacc tttggtcgaa aaaaaagccc gcactgtcag gtgcgggctt ttttctgtgt
    15181 ttcctgtacg cgtcagcccg caccgttacc tgtggtaatg gtgatggtgg tggtaatggt
    15241 ggtgctaatg cgtttcatgg atgttgtgta ctctgtaatt tttatctgtc tgtgcgctat
    15301 gcctatattg gttaaagtat ttagtgacct aagtcaataa aattttaatt tactcacggc
    15361 aggtaaccag ttcagaagct gctatcagac actctttttt taatccacac agagacatat
    15421 tgcccgttgc agtcagaatg aaaagctgaa aatcacttac taaggcgttt tttatttggt
    15481 gatatttttt tcaatatcat gcagcaaacg gtgcaacatt gccgtgtctc gttgctctaa
    15541 aagccccagg cgttgttgta accagtcgac cagttttatg tcatctgcca ctgccagagt
    15601 cgtcagcaat gtcatggctc gttcgcgtaa agcttgcagt tgatgttggt ctgccgttgt
    15661 gtcacttttc gccggttgtt gtattaatgt tgctaattga tagcaataga ccatcactgc
    15721 ctgccccaga ttgagcgaag gataatccgc caccatcggc acaccagtaa gaacgtcagc
    15781 caacgctaac tcttcgttag tcagcccgga atcttcgcga ccaaacacca gagcggcatg
    15841 gctcatccat gaagattttt cctctaacag cggcaccagt tctactggcg tagcgtagta
    15901 atgatatttc gcccgactgc gcgcagtggt ggcaacagtg aaatcgacat cgtgtaacga
    15961 ttcagctagt gtcttgaaaa ctttaatatt atcaataata tcaccagatc catgtgcgac
    16021 ccaacgggcg actggctcca ggtgtgcctg actatcaaca atccgcagtt cgctaaaacc
    16081 catcgttttc attgcccgcg ccgctgcccc aatattttct gctctggcgg gtgcaaccag
    16141 aataatcgtt atacgcatat tgccactctt cttgaccaaa taaccgcgaa cagggtgatc
    16201 attgtcaact tattacgcgt tgcgaattta caaattctta acgtaagtcg caggaaaagc
    16261 cctttgctta gcttaaaaaa agctaaacta tttcctgact gtactaacga ttgaattgtt
    16321 aaaaaatgct acatatcctt ctgtttactt aggataattt tataaaaaat aaatctcgat
    16381 aattggattc accacgttta ttagttgtat gaagcaacta gttggattat taaaataatg
    16441 tgacgaaagc tagcatttag atacgatcat ttcatcaaac tgttaacgtg ctacaattga
    16501 acttgatata tgtcaacgaa gcgtagtttt attgggtgtc ctgcccctct tagcctgtta
    16561 tattgctgtt aaaatggtta ggatgacagc cgtttttgac actgtcgggt cctgagggaa
    16621 agtacccacg accaagctaa tgatgttgtt gacgttgatg gaaagtgcat caagaacgca
    16681 attacgtact ttagtcatgt tacgccgatc atgttaattt gcagcatgca tcaggcaggt
    16741 cagggacttt tgtacttcct gtttcgattt agttggcaat ttaggtagca aacatgcaga
    16801 ccccgcacat tcttatcgtt gaagacgagt tggtaacacg caacacgttg aaaagtattt
    16861 tcgaagcgga aggctatgat gttttcgaag cgacagatgg cgcggaaatg catcagatcc
    16921 tctctgaata tgacatcaac ctggtgatca tggatatcaa tctgccgggt aaaaacggtc
    16981 ttctgttagc gcgtgaactg cgcgagcagg cgaatgttgc gttgatgttc ctgactggcc
    17041 gtgacaacga agtcgataaa attctcggcc tcgaaatcgg tgcagatgac tacatcacca
    17101 aaccgttcaa cccgcgtgaa ctgactattc gtgcacgcaa cctgctgtcc cgtaccatga
    17161 atctgggtac tgtcagcgaa gaacgtcgta gcgttgaaag ctacaagttc aatggttggg
    17221 aactggacat caacagccgt tcgttgatcg gccctgatgg cgagcagtac aagctgccgc
    17281 gcagcgagtt ccgcgccatg cttcacttct gtgaaaaccc aggcaaaatt cagtcccgtg
    17341 ctgaactgct gaagaaaatg actggccgtg agctgaaacc gcacgaccgt actgtagacg
    17401 tgacgatccg ccgtattcgt aaacatttcg aatctacgcc ggatacgccg gaaatcatcg
    17461 ccaccattca cggtgaaggt tatcgcttct gcggtgatct ggaagattaa tcggctttac
    17521 caccgtcaaa aaaaacggcg ctttttagcg ccgtttttat ttttctacct tatttccaga
    17581 tacgtaattc atcgtccgtt gtaacttctt tactggcttt cattttcggc agtgaaaacg
    17641 cataccagtc gatattacgg gtcacaaaca tcatgccggc cagcgccacc accagtacac
    17701 tggttcccaa caacagcgcg ctatcggcag agttgagcag tccccacatc acaccatcca
    17761 gcaacaacag cgcgagggta aacaacatgc tattacgcca gcctttcaat accgcctgca
    17821 aataaatacc gttcattaac gccccaatca gactggcgat tatccatgcc acggtaaaac
    17881 cggtatgttc agaaagcgcc agcaagagca aataaaacat caccaatgac agccccacca
    17941 gcaaatattg cattgggtgt aaacgttgcg cggtgagcgt ttcaaaaaca aagaatgcca
    18001 taaaagtcag cgcaatcagc agaatggcgt acttagtcgc ccggtccgtt aattggtatt
    18061 gatcggctgg cgtcgtcact gcgacgctaa acgcggggaa gttttcccag ccggtatcat
    18121 tgcctgaagc aaaacgctca ccgagattat tagcaaacca actactttgc cattgcgcct
    18181 gaaaacctga ctcgctaact tcccgtttgg ctggtagaaa atcacctaaa aaactgggat
    18241 gcggccagtt gctggttaag gtcatttcgc tataacgccc gccaggcacc acagaaagat
    18301 cgccggtacc gcttaaattc agcgccacat tcagcttcag gttctgcttg cgccagtccc
    18361 cttcgggtaa agggatatgc acgccctgcc cgccttgctc taacccggtg ccgggttcaa
    18421 tggtcagcgc cgtaccgtta acttcaggcg ctttcaccac accaatacca cgcgcatccc
    18481 cgacgctaat cacaataaat ggcttgccta aggtgatatt tggcgcgtcg agttcgctaa
    18541 gacgcgaaac atcgaaatcg gcttttagcg ttaaatcact gtgccagacc tgaccggtat
    18601 agatgcctat cttgcgttct tccacgttct gattgccatc aaccatcaat gactcaggta
    18661 accaaaaatg aataaaactt cttttccgct ccacggtttt atcgtcttcc tgcaccgtat
    18721 aaagctcggt cacaggaata gcgatgagcg gcccaacgag tttttgcggc ccgctggtac
    18781 tttggcgaat cacatcttcc acatcgctac ggtaatcggc acgttcgaca atcacctgcc
    18841 gaatcagcat tatcggaatc aacaacagca atactgcacc aaacaaggtg gtcattttcc
    18901 agaacagggg ggatttcaac atagtcttct cctttgcaat ggcagcagga tacgaaggct
    18961 atgtgggaag aatttgaagc tatgtgaagt gacggtgaag tcgaagcgag gccagcacgc
    19021 caccttcctg cacgttgcgc agcgtgactt cgccgttaaa caaacgggcg acctcactga
    19081 caaacgccaa ccccagaccg ctgctttttt gcccatttgc acgcggcaaa gagtaaaagc
    19141 gttcaaaaat acgtgacagc gcgtagtcag gaatcccact accggtatcc agcaccttaa
    19201 gggtgacgta ttcctgatcc acttcggcgc ttagcgttat gcaaccgctc tcgggagtaa
    19261 aatcgatggc gttatccagt aaattcccca gcgcctgctc cagtaacgcc ggttcagcag
    19321 caacgttaac ctcagtaggc ataacatgca aagtgatgtt tttttctgcc aactgcacgg
    19381 tgcgcgcttc gctgacgcgg cgaaataatg ccgccacatc aacagcagtc agaacgactt
    19441 cctgacgatt ctccagtctt gcctggcgta gtaacgtttc caccagtgcc tgcattcgcg
    19501 cattttgcgt cagaatgttg tcggtaaaac gagccaccac ttccggcggc ggaccttcgc
    19561 ataaaatttc cgccgcgcca cgaatcgccg ccagtgggct ttttagctca tgagttaacg
    19621 cataaacata ctgctcaata tagtttttcc cttccagctt cacgcgcata ctttccagcg
    19681 cctgcgcgag tttacgcaac tcgctactac cgagatcggg gagaggaacg ggcttattgt
    19741 cagtgacgga atcagcatag cgagtgagcc gggcaataga gcggttgatc caccaaacca
    19801 tgcctgcgcc aatcaccagt gcaatcccca acaaaatggc gctggcccat aaaattcgcc
    19861 gctcgctacg cttaatgacc ggagccatcg ccgcgttcgg tttgcctacg ctcaaaacgc
    19921 caataagccg cgagccgtcc ataatcggcg cggcaacata catcacagaa ctttcgggat
    19981 cggcaggatt ttgcaacgtg ctgcgcgcac catactgacc acgcaacgtt agccagacgt
    20041 cattccagcg cgaataatcc tgtccaacgg ctttatttgc tgaatcaaac aatactttgc
    20101 cctgcgcatc ggtcatatag acatgatatt cgttgcgcac tttgttaatg ccaccgatat
    20161 tggcgcgaaa cgggcgatgt tgtagctgat taaacgcctg cgccagttgc ccatgcgttg
    20221 ggtccccaga gagcaaatcg ggacgcgcca gctccgccag caacgttgcg gtgtcgatta
    20281 acgtcccctc ggttgctctt cgcacgcccg gtttaacttc tttgacaaaa atagccagaa
    20341 cgaaccaggc cgccaccgcc accagtaaaa aatagcccag caacaaccgc atgccgatac
    20401 gcattacagg cccctcaggc tatatcccat gccgcgatga gtattaatcg gtgaaagatc
    20461 ggggttgatg gcgcgcagct tggcacgcag cgttttaatg tgggtatcga cggtgcgatc
    20521 gtaggtgtcc tgcgcatctt cccacacgct atccattagt tgctggcggg accagacgcg
    20581 gcccggcgac ttgagtaacg tcttcagcaa taaaaactca taccgcgtca gcgttaatgg
    20641 cgtgtcaaac cagctgatct gcgccgcagg ttcattcaat tcaaaatgac caatacggat
    20701 aacgggagac ggcgtcgaga acttcttcac ccgacgcagt aaggtgcgca ctctggcgca
    20761 cacttcgcga ggtgaaaacg gtttagccac gtagtcgtca gcgccaattt ccagcccaag
    20821 caggcgatcg acctcttcat ttcgggccgt caggaacagt acaggtaacg ccggatggag
    20881 cgccagtaac tggcggcaca attcaaagcc gctgatatca ggcaaaccaa catcgagaat
    20941 catgacatcg ggaacctgct ggcgagcttt atccagcact ggcaagcctc gctcaaagac
    21001 ctcgacgtca aaaccttcct gttgcaacat gtagaccagc gtatcggcta tcccttgctc
    21061 gtcttcgact aaccagaccg tttcccgttg cataaatcgc ctctgttatt gccgccacgg
    21121 catgacagga accgcgctaa tcgcgttttt cggcgaacct tctacaactt tgtcggagta
    21181 agccagataa gcgagcgcgt tgcgtttagc gtcataaaag cgcacgacct gtaacgactt
    21241 aaagaccagg gacgtgcgtt ttttgaatac tacctcgccc tgagctttgc cgtttttaat
    21301 acgatccgac agttcaatcg gcccgacttg ttgacaagaa atggccgcat cggaggtatc
    21361 ttctgccagg cccaatcccc ctttaatacc accggttttc gcccggctca cataacaggt
    21421 gacatttttc acatcgggat cgtcaaaggc ttcaacaaca attttgtgat ccgggccgat
    21481 cattttaaat acggtgtcga ccgaaccaat ctcttctgca tgagccaatg gccccagcat
    21541 tatcaggctt aaagacagga ttaaatgctt gtatttcata tcgttaccat ttttatctat
    21601 tacacgagca attagttcgt cacggtaaat aacatattga ttaagaacaa aatctcaata
    21661 cttttatttc cgtcaagacc taaaacatac tcgactaaga aaaaaacact gaatgctaaa
    21721 acagcaaaaa atgctattat ccaattacct gatgtcaggt gctcgttgtt gaaaggatga
    21781 ggatatttta tggatcaggc cggcattatt cgcgaccttt taatctggct ggaaggtcat
    21841 ctggatcatc ccctgtcgct cgacaatgta gcggcgaaag caggttattc caagtggcac
    21901 ttacagagaa tgtttaaaga tgtcactggc catgctattg gcgcgtatat tcgtgctcgt
    21961 cgtttgtcga aatcggcggt cgcactacgc ctgactgcgc gtccgattct ggacatcgcg
    22021 ctgcaatacc gcttcgactc tcaacagaca tttacccgcg ctttcaagaa gcagtttgcc
    22081 cagactcctg cactttaccg ccgttctcct gaatggagcg cctttggtat tcgcccgccg
    22141 ctacgtctgg gtgaattcac tatgccagag cacaaatttg tcactctgga agatacgccg
    22201 ctgattggtg ttacccagag ctactcctgt tcgctggagc aaatctctga tttccgccat
    22261 gaaatgcgct accagttctg gcacgatttt ctcggcaact cgccaaccat tccaccagtg
    22321 ctttacggtt tgaacgaaac acgtccgagc caggataaag acgacgaaca agaggtgttc
    22381 tacaccaccg cgttagctca ggatcaggca gatggctatg tactgactgg gcatccggtg
    22441 atgttgcagg gcggcgaata tgtgatgttt acctatgaag gtctgggaac cggcgtgcag
    22501 gagtttatcc tgacggtata cggaacgtgc atgccaatgc ttaacttgac gcgccgtaaa
    22561 ggtcaggata ttgagcgata ctacccggca gaagatgcca aagcgggaga tcgcccaatt
    22621 aatctgcgct gtgaactgct gattccgatc cgtcgttaac gctgcagctc atctaatgca
    22681 ggggcgtcca gatgcgagat gtcccctgcg gtttcaacca cccagccaga cgccagccac
    22741 aagctttcct gataatccac gcgcgaaata gaacagttac gcagacgtaa gcggcgctct
    22801 gcccatgctg gtaatccgag aatcgtactc accaggcatc ccagtgcaat accatggctc
    22861 accagtagcg gtcggcttcc ctgcggtaag tcccggcaag attccagtgc cgcattaacg
    22921 cgatcgctga gctcctgcat tgactcgcct tcaggaatac gcccgtcaac ggtgccattg
    22981 accagttgcc gacgccagtt ctcttcttct tcggtgagtg aatcgatatt tcttgtttcc
    23041 agtacaccca tatttaattc acgcaggcga gaatcaaaga tgatgtcaca gccgcaggcc
    23101 tgggcgatga tttccgccgt gcgccgggtc cgtcctaaat cgctactgat gatatgcgta
    23161 atgccaagct ctttggcacg ggttgccacc tgcatcgcct gttgctcacc tttggcggtc
    23221 agcgggctgt cagactggcc ctgaatacgt cgctcggcgt tccactgcgt ttcaccgtgg
    23281 cggactaggt atacctgtaa catgcttttt ttccgtatac tgctgtaacg ttaattttct
    23341 gagtaatgct gattatgcac caagttgtct gtgcgaccac caatcccgct aaaattcagg
    23401 ccattctgca ggcatttcac gagatcttcg gcgaaggatc ctgccatatt gcatccgttg
    23461 ccgtcgagag cggtgtaccg gaacagccct ttggcagtga ggaaacgcgc gctggcgcac
    23521 gaaatagggt agccaatgcc cgccgtttac ttcctgaggc tgatttttgg gtggcgattg
    23581 aagctggcat cgatggtgac agcactttca gttgggtggt gatagaaaac gccagccagc
    23641 gcggcgaggc gcgttcggct actttgccgt tgcctgcggt gattctggaa aaagtgcgtg
    23701 aaggtgaggt gcttggtccc gtgatgtcgc gttataccgg tattgatgag attggtcgta
    23761 aggaaggggc gatcggcgtg tttaccgccg ggaaactcac tcgcaccagc gtctatcacc
    23821 aggcggtgat cctggcgctg agtccgtttc ataatgccgt gtatcagcca ttgcaggcct
    23881 gattaggcgt tcacgccgca tccggcattc ggtgcacgat gcctgatgcg ccatgtccta
    23941 tcaggcctac aaaatcaatc gcttttcagc aacacgtctt ccagccactg gcgcagctcg
    24001 acgggcgcgg ctttcagact attagatcca cgcgtaatcg tcgcgatgcc cgcgccgagt
    24061 tcatttttta actcacgctg gctcatttcg ccgcgcaaca gctcttcgac aatacgcacg
    24121 cgagtcccca acgcttcgcg ctcatctggc gtcagcatca ggtttaacaa cggtaaatgg
    24181 agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac gtaaccactc ctggtgacgc
    24241 tgttctgcca tcgctgctga atagggtgat tgttgggcca tagtatgtcg ccattgttag
    24301 cgggggaagc aaaatgcctc ccccggttgt actcgctaaa gagtacgata gcatatcata
    24361 aacgtgtgga tcagtaacga cgtccccatt ccgtggcgct catcaacgtc ggtttatccc
    24421 ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt tttcacataa ccgcgcgtct
    24481 cggagaatgg aatactctcg acaaatgcca ctgcgtcgat acgccctgcg ctgttgccaa
    24541 gccaggttcg cacccgccct ggtccggcgt tataagctgc tgaggagaaa atacggttat
    24601 tgccaaactg ctgataaaca tattgcaggt aactggtgcc aatgttgatg tttgtttccg
    24661 gatccagcaa ttgcccaggg ctgctataac cgggaataga gaacatcttc accgtatggg
    24721 tcgcggtacc aggcataatc tgcatcaggc cgctggcccc taccggtgat ttcactttcg
    24781 gattccaggc actctcctga cgggcaatcg ccatcgcata gctttgcggg atctccttac
    24841 cactggtgta gcgtttgaaa agatcgttgt aagccagcgg gaatcgctct tccagatgat
    24901 cccacagctt cccggcgatc gttgcctgaa cgctaagatc ccaccattgg ttattgaaag
    24961 cataccgagc cagttgagcc tgctctgttt ttgacttgct cttcaccaga ttggcccact
    25021 cgctacgcgc ggtgttatcg agattccagt acatcaactc gcgcacgcgc gccatctccg
    25081 gcccctgagt cagggcgctg tcaacattct gcggcgcttt atcaatcttc agctcatact
    25141 cttcgccgat gcgttgtgca gcaaccatcg ggtagaaacc acgctgttgc atgagttgat
    25201 gcaaaatctc ttttgcttca gcttcacgtc cgcgttccag caataaatcc gcctgccagt
    25261 aacgccattc atctttctct ttcgcttcca taggcagacg cgccagccag gtattcaggc
    25321 cgcgtcgatc gccggtgcca agcgccattc gcacacggcg ttcaataagc gaagtagatt
    25381 gcgagcgcat aatcgcgtca tcgcgccatt tcgcctgctc gtcggtgaca tcgttgccca
    25441 tcaaacgcca ggcgacaata tcgcgcagct cctgaatctg atcttcatta agctgctgtg
    25501 cctgagcaag cgacgggatc atcagccgtg cattttcagc atcctgccgc gccacactgg
    25561 caaacgccac cgccgccatt tgacgggtaa aatcggtcgc gccggttgta cgcgcgaagg
    25621 tcagtaccgt attagggttg ttcgccagtg aaatgattgc cgaagcgata gtctggtaat
    25681 cggcaggcat ctgccctgcc agcactgtta ccaggccggt gttacccgct ttcatcgcca
    25741 gacggatacg ctctaaatac gccagcggat cttgtttacc tgacgcacgc cagacgctaa
    25801 ataacttgtc acaggcgtta ggctggctct tgccggttag ccacagctct ttcgcccctt
    25861 gccaggcttc ttcactctgc ccggtgttcc atttcgcata gtagtaatta cattgcgctt
    25921 cggtagttcc gggtttttcc gggctaaagg ctaacaagcc acgccagtct tcacgccgcg
    25981 ccagttcatt gacgaaacga gattgcagcg tgcgagcggg gggaagcgtg gggttagcgc
    26041 taacaaagtt agtgaccgtc accgccggtt gattcatcag gtcatcagtg atctggcggt
    26101 attccaggta gggataaagt gggtaatctt tcagtccagg catcatttgt tcgaccacat
    26161 ccatttgtcg attatcccag gcctgcttaa tttgcgcgta acggctacgc tgctcatcca
    26221 gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca gacaccgaca gccaacagcc
    26281 gccaggtaac gtgtttggct ttttccacaa gcgcatcctc taagtgtaaa tacatcaatg
    26341 cagcgtcatg ccgcgtaata gatttatgct aaccagtcat tgccgtttac gccacgttac
    26401 ggacactttt ttacttttac ggggtgatcg actggctggg attagacagg gaaacaggct
    26461 acacttcgcc tttgaaaact catcatcacg ataaacatag aggcgaagtc caacgtggct
    26521 caattcgttt ataccatgca tcgtgtcggc aaagttgttc cgccgaaacg tcatattttg
    26581 aaaaacatct ctctgagttt cttccctggg gcaaaaattg gtgtcctggg tctgaacggc
    26641 gcgggtaagt ccaccctgct gcgcattatg gcgggcattg ataaagacat cgaaggtgaa
    26701 gcgcgtccgc agccagacat caagattggt tatctgccgc aggaaccgca gctgaacccg
    26761 gaacacaccg ttcgtgagtc cattgaagaa gcggtttctg aagtggttaa cgccctgaaa
    26821 cgcctggatg aagtgtatgc gctgtatgcc gatccggatg ccgattttga caagctggcc
    26881 gctgaacaag gccgtctgga agagatcatt caggctcacg acggtcacaa cctgaacgtt
    26941 cagctggagc gtgcggcgga tgcgctgcgt ctgccggact gggacgcgaa aatcgctaac
    27001 ctttccggtg gtgagcgtcg tcgcgtggcg ttgtgccgcc tgctgctgga aaaaccagac
    27061 atgctgctgc tcgacgaacc gaccaaccac ctagatgcgg aatccgtggc gtggctggaa
    27121 cgcttcctgc acgacttcga aggcaccgtg gtggcgatta cccacgaccg ttacttcctc
    27181 gataacgttg cgggctggat cctcgaactt gaccgcggtg aaggtattcc gtgggaaggt
    27241 aactactcct cctggctgga gcagaaagat cagcgtctgg cgcaggaagc ttcacaagaa
    27301 gcggcgcgtc gtaagtcgat cgagaaagag ctggagtggg tacgtcaggg aactaaaggc
    27361 cgtcagtcga aaggtaaagc acgtctggcg cgctttgaag agctgaacag caccgaatat
    27421 cagaaacgta acgaaaccaa cgaactgttt attccacctg gaccgcgtct gggcgataaa
    27481 gtgctggaag tcagcaacct tcgtaaatct tacggcgatc gtctgctgat tgatgatctg
    27541 agcttctcga tccctaaagg ggcgatcgtc gggatcatcg gtccgaacgg cgcgggtaaa
    27601 tcaaccctgt tccgtatgat ctctggtcag gaacagccag acagcggcac catcacttta
    27661 ggtgaaacgg tgaaactggc atctgtcgat cagttccgtg actcaatgga taacagcaaa
    27721 accgtttggg aagaagtttc cggcgggctg gatatcatga agatcggtaa caccgagatg
    27781 ccaagccgcg cctacgttgg ccgctttaac tttaaagggg ttgatcaggg taaacgcgtt
    27841 ggcgaactct ccggtggtga gcgtggtcgt ctgcatctgg cgaagctgct gcaggttggc
    27901 ggcaacatgc tgctgctcga cgaaccgacc aacgacctgg atatcgaaac cctgcgtgcg
    27961 ctggaaaacg ccctgctgga gttcccgggc tgcgcgatgg ttatctcgca cgaccgttgg
    28021 ttcctcgacc gtatcgccac acacattctg gattaccagg atgaaggtaa agttgagttc
    28081 tttgaaggta actttaccga gtacgaagag tacaagaaac gcacgctggg cgcagacgcg
    28141 ctggagccga agcgtatcaa gtacaagcgt attgcgaagt aatgcgtaaa atgccggatg
    28201 cggcgcgcca gcatcgcatc cggtaatagt taaatgatta atgggaggca agttcccgga
    28261 aaaaggcagg attttcttta attgtggcaa gtacttttgc agccaatccc gtaggatttc
    28321 ttcgcttcga ttcccagctt ttaatggtat ccacactggt tcccagagct tttgccatct
    28381 cactttgaga aacgttgagt tgctcacgaa tagctttaac atccgcaatt tcgtagcggg
    28441 tcacccgtgc aggttctttt aaaccttgtt taatctcgac agcctcttcc agagaggttt
    28501 tcaactcatc aaaaaaactc attttatatc tcgcctttga gcagtttcgt caacttcttt
    28561 agttgagctt tttccgtatc tgtcagacta tctttggcat ttttcggata ggccataatc
    28621 aaataaataa tctcttccgt cgccagaaag taaattattc tgacactgcc actttttccc
    28681 tgtgaacccg cagccattcg gactttacgc aatccaccag tctgctgaat gagatcacct
    28741 ttatcgggag ttccgatgag ttctttttgt aattccttta attcatcatc ggttgcaatc
    28801 tgcttgatct gacgcgtaaa cattggcgtc tctataaata ttatcgcctt acccattccc
    28861 cctccctggc aggtacattg tacacctaaa tggagtacta agtacacttt taaatcagta
    28921 atcccactca acatcttata aagatgatta tctttgctcc cccatcatct cccgcaccag
    28981 ctccacgcag cgcaggaaac ggctgtcgta atcgtcctct tcaacccgca cgaattcgat
    29041 attgttctct tccagcatct ccaccagcaa gttctggaac tctttgcgat ccaccgaact
    29101 gccgaggctg cgtaaaccat ccgccaccca cggcgtgttg ttctccagca ggatcaccag
    29161 atcgaaacgg tattcatcaa tcaacgcctg cacgaacgga tgctcacgcc cttcgtactt
    29221 tttgcagaac gcctgggtag tgacaaaatc ggtgtcgata aacgccactt tattggcata
    29281 tttcactgca aaatcaatat attgcgcgtg tcccagcgcg attttatcgt agtcggaata
    29341 ctgcaacgca atctcatcac cgccaaggtg tgaaaagaca taatcgcgac catattccca
    29401 tgcactggta gtgttgaaga tattggcaag tttgtttacc agggtggatt taccgctcga
    29461 ctcgccgcca aggatcgcca cggtacgcac aaagaacggc ttaacttccg taggaatata
    29521 ttcccagtag cggaacgggt tttcgcggat ctgcgcaccg ctgatactca taaaggtacg
    29581 cttcggatcg accagcaccg tctcgatccc cagatgttcc atatactgcg gcgcatcggc
    29641 ttcttccgag gtgtagatca ggtccggctg aatgcctttt tcggccataa actttttgat
    29701 gccgttgctc cacacatccc agccatgtgg atacggttcc atgccctctt cgttaaaagc
    29761 atgaatgcgg atattttttt gatacttaaa ggtctgcaat aaccagcgca gacgatccgg
    29821 cacggttggc tgctgcgaca tggcgctgtc ttcgaacaat gcgcggtcgc gggtatcgtc
    29881 aaaacccata atgatatgca gctcgtcaac ctggctacag gcgcgctgga taaggtagat
    29941 atgtccagta tgcagcgggt aaaacttacc gaatacgaca ccgatcgttt tcttctgccg
    30001 gggaaattca agtcccaaat aacggtgcaa tgcctccagc ttttgtgcgc tggggctttt
    30061 aattttggca ttcagtaact ggcttaaata ccctttggtc ataccgctgg catcagctac
    30121 ctgctgtagc gtgcagccct gttgcttgat ggcagttttc aggtaatcaa atgacgacat
    30181 atctccctcc gtatctctca ttataagtcg tcgaacacgc taagcgcgtc ggagagtttt
    30241 ttaacgccaa aaatctgcat cccttccggc gcttttttcg gcacgttagc tgccggaaca
    30301 attgcccggc gaaaaccgtg tttcgccgct tctgagattc gttcctggcc gctaggcacc
    30361 gggcggatct cccctgccag cccgacttca ccaaacacca ccagatcctg cggtagcggt
    30421 ctgtcacgca ggctggaaac catcgccagc agtaacgcta aatcggcact ggtttccgtc
    30481 accttcacgc caccgaccac gttaacaaac acatcctgat cggccatttg tagaccaccg
    30541 tgacggtgaa gcaccgccag caggattgcc agacggtttt gctccagccc gactgccacg
    30601 cggcgtggat tcgccatcat cgagtgatcg acgagcgcct gaatctccac cagcagcgga
    30661 cgcgttcctt cccacactac catcactgag ctaccggagg tcacttcatc gccgcgactt
    30721 aagaaaattg ccgaagggtt gctgacttca cgcagcccct gctcggtcat tgcgaagaca
    30781 cccagctcat tcaccgcgcc gaagcggttt ttatggctgc gtaaggtgcg aaaacgtgag
    30841 tcggcatcac catccaaaag caccgaacag tcgatacagt gttccagcac tttagggcca
    30901 gccagcgagc catctttggt tacgtgcccg accatgacaa tcgccacacc gcgcgttttg
    30961 gcgaagcgcg tcagataagc cgccgtttca cgcacctgtg ccacgctgcc aggcgatgat
    31021 tgtacatccg ccatatgcat cacctggatg gagtcaatta ccatcagctt cggttgctct
    31081 tcttcggcaa tcaggcagat ctgctcaatg ctggtttccg acagcatatt aagatagtca
    31141 gtcggcaggc caaggcgatg agcgcgcatt gccacctgtt gcagcgactc ttcgccggtg
    31201 acatacagcg ttttcatctg ctgcgccagt ttgcacagcg tttgcagcaa cagcgtggat
    31261 ttccccgcac cagggttacc gccaatcaga atggcgcttc ccggcaccac gccgccgcct
    31321 aatacgcggt cgaactcttt aaatccggtg gaaaaacgcg gcagctcttc aaggctgatg
    31381 tcggaaagtt tctggacttt tgccactccg gcgctaccgg catagccgct gagacgctcg
    31441 ttacgcgcca ccgttggcga cgctgcaaga cgcacctcgg tgatggtgtt ccaggcatga
    31501 caggcactgc actgcccctg ccagcgcgga taatcggccc cgcattcatt acaaacaaag
    31561 gcgcgttttg gagcttttgc cacgatgtac ctcgttaatg ctgtgccgcc cgcaggatga
    31621 cgggcggaca attacttctg attcaggctg ccagagagga tgcagaatac ccccatcagg
    31681 tcagcgtgac ggatggtgac ttccgtcttt tcattcactt ttggcttggc atggtaggca
    31741 atccccagcc ctgccgcttt gatcatcggc aggtcattgg ctccatcgcc aatcgccacg
    31801 gtctgcgcca gcgggatttc atactcctgc gcgaggcggg tcagggtttt cgctttatat
    31861 tgcgcgtcaa cgatgtcgcc gatcacattg ccggtaaatt taccgtccat gatctccagt
    31921 tcattggcta ccacggctgt caggcgcagc ttgtcgcgca ggtattcagc aaagaaagta
    31981 aagccgccgg aggcaatcgc cactttccag cccagcgttt ccagcttgag taccagctgc
    32041 gttaagccag gcatcagcgg cagattttca cgcacctgtt gcagaatatt ggcgtcagcg
    32101 cctttcagcg tcgccacgcg gctgcgcagg ctggcggtaa aatcgagttc gccgcgcatc
    32161 gcccgttcgg ttacctccgc caccctctcg cccgttccgg ccagtttggc aatttcatca
    32221 atacattcaa tctggatggc ggtggaatcc atgtccatca ccagcaaccc cggcgtgcgc
    32281 aggtgtggga ttttccccag cggcgcgaca tccagttgcg cttcatgggc caggcgcgta
    32341 gcccgcgcag tgagtgaacc tgccagacga atcacctgat aatcttccac gcaccaggcg
    32401 gcaacaatca ccatagccgc gcccagtttg ctctggtatt gggtcagacg ttgtttatcc
    32461 agcccacgac catacagcag ccagccgcta cgacctgcgt ggtaatccag tggcatcact
    32521 tcatcaccac ttaatgaaag aggcagacca ggccataaag agacatcttc aggcaggtcg
    32581 caccaggtaa tgttaggcat taaggctcct gtaaaatcgt tcgaagcagg gaaaataacg
    32641 catgaggcta ccttgtatcc attgcttctg gcaacattaa gtctcaaatt ttcaaagggt
    32701 ggaagatggc tcgcacaaaa ctgaaattcc ggctgcatcg ggcagtgatt gtcctgttct
    32761 gtcttgcctt gttagtggcg ctgatgcagg gggcgtcatg gtttagtcaa aaccaccagc
    32821 gacagcgtaa tccgcaactg gaagaactgg cccgcaccct ggcgcggcag gtgacgctaa
    32881 acgttgcacc gctgatgcgt accgactcac cggatgaaaa acgcattcag gcgatcctcg
    32941 atcagttaac ggatgaaagc cgcattctcg acgcaggcgt atatgatgag caaggcgatc
    33001 ttatcgcccg ttctggcgaa agcgtcgacg tgcgcgaccg gctggcgctc gacggcaaaa
    33061 aagcgggcgg ctattttaac cagcagattg tcgaacccat tgcgggtaaa aacggaccgc
    33121 tcggctatct gcgcctgacg ctcgacaccc atacgctcgc caccgaagcc caacaggtgg
    33181 ataacaccac taacatttta cgcctgatgt tgctgctctc gctggcaatc ggcgtagtgc
    33241 tgacccgcac gctgctacag ggtaaacgca cccgctggca gcaatcgccc ttcctgttaa
    33301 ccgccagtaa accggtgccg gaagaggaag aaagcgagaa aaaagagtga cccattacta
    33361 caagaaagga aatcgttatg tccacattac gcctgctcat ctctgactct tacgacccgt
    33421 ggtttaacct ggcggtagag gagtgtattt ttcgccagat gccggctacg cagcgcgttc
    33481 tgtttctctg gcgcaatgcc gacacggtag tgattggtcg cgcgcagaac ccgtggaaag
    33541 agtgtaatac ccggcggatg gaagaagata acgtccgcct ggcgcggcgc agtagcggtg
    33601 gcggcgcagt gttccacgat ctcggcaata cctgttttac ctttatggct ggcaagccgg
    33661 agtacgataa aaccatctcc acgtcgattg tgctcaatgc gctgaacgcg ctcggcgtca
    33721 gcgccgaagc ttccgggcgt aacgatctgg tggtgaaaac cgccgaaggc gatcgcaaag
    33781 tctcaggctc ggcctatcgc gaaaccaaag atcgtggctt ccaccacggc actttgctgc
    33841 tcaattccga ccttagccgc ctggcaaact atctcaatcc ggataaaaag aaactggcgg
    33901 cgaaaggcat tacctcagtg cgttcccgcg tgaccaatct gaccgaattg ctaccgggga
    33961 tcacccatga gcaggtttgc gaggccataa ccgaggcctt tttcgcccat tatggcgagc
    34021 gcgtggaagc ggaaatcatc tccccggaca aaacgccaga cttgccaaac ttcgccgaaa
    34081 cctttgcccg ccagagtagc tgggaatgga acttcggtca ggctccggca ttctcgcatc
    34141 tgctggatga acgctttacc tggggcggcg tggaactgca tttcgacgtt gaaaaaggcc
    34201 atattacccg cgcccaggtg tttaccgaca gtctcaaccc cgcgccgctg gaagccctcg
    34261 ccgggcgact gcaaggctgt ctgtaccgcg cggatatgct gcaacaggag tgcgaagcgc
    34321 tgctggttga cttcccggaa caggaaaaag agctacggga gttgtcagcg tggatggcag
    34381 gggcggtaag gtaactcgct gattaaaata tccagggaag gaaatatgat cgatctggaa
    34441 aaccaggaac gggagattat caatttaatg ttcagccagg atatatcctg gctaaccgcc
    34501 gtgcgtatca ggcataaact atcacttgct gaagtaagta aaatgttagg tatctccatt
    34561 aattctctaa aacagatcga aaagacggaa cggcttagtt caaacatcaa aagcaaaatg
    34621 gcaggaattt atggttgtcc accagagttg ctgatatgcc cttcctggat gactgctgaa
    34681 cataaatgaa tagattcgtg aatagatact tgaatggatt ttattttcta tccatatgaa
    34741 aaataatagt aaatctataa atcagttgct attgatgaat aaatactaaa aagccggagc
    34801 agtttcccac tccggcttca cacgacaatc gcctttcagc aaaacacaat tactctttat
    34861 cgcccagcag aacggattcc agcgcgattt tgatcatgtc gttgaaggtg gtctgacgct
    34921 cagcggcagt ggtctgctcg tgagtgcgga tgtggtcaga cacggtgcag atggtcaggg
    34981 ctttcgcgcc aaattctgca gcgacgccgt agataccagc cgcttccatt tccacgccga
    35041 ggatgccgta tttttccatc acgtcgaaca tttcgccgtc cggagagtag aacaggtcag
    35101 cggagaacag gttaccaaca cgagcatcaa cgcccagtgc ttttgccgcg tctaccgcgt
    35161 tacgcaccat gtcgaagtca gcgatagcgg caaagtcatg gtctttaaaa cggatgcggt
    35221 taactttgga atcggtgcag gcacccatac cgataacgac gtcgcgcagt tttacgtgcg
    35281 gcagaacagc gccacaggaa ccaacgcgga taattttctt cacgccgaaa tcagtgatca
    35341 gttctttggt gtagatggag caggacggga tacccatacc gtggcccatt acggaaattt
    35401 tgcggccttt gtaagtacca gtgaagccca gcataccgcg aacgttgttc acttcacggg
    35461 catcttcaag gaaagtttca gcaatatact tcgcacgcag cgggtcacct ggcatcaaaa
    35521 ctacgtcagc aaaatcgccc atttctgcat taatgtgtgg agtagccatc attttatcct
    35581 ttttgtgaca tattaaaggc ggagtcactg ctccgcccaa atccatcaga acatggcttt
    35641 gccatattcc atatcagaag taccaaaata ttttgccaga gtctggccga tatccgcgaa
    35701 ggtttcacga tgacccagcg aacctggttt cactttcggg ccgtaaacca gtaccggaat
    35761 gtgttcacgc gtgtggtcag taccggtcca ggtcgggtca caaccgtggt cagcagtgag
    35821 aatcagaata tcgtcatcgc gcagcaaaga catcagctcc ggcagacggc ggtcgaacag
    35881 ttccagaccc gcagcataac cggcgacgtc gcgacggtgg ccccaggaag agtcgaagtc
    35941 aacgaagttg gtgaagacga tggtgttatc acccgcttct ttcatctctt tgatggtggc
    36001 gtcaaacagc gcgtccaggc cggtcgcttt cacttttttg gtgataccgc agttggcgta
    36061 gatgtctgca attttaccga ccgaaaccac ctggccgtgt ttttcatcaa ccagtttctg
    36121 cagcacggtc ggtgctggcg gctcaacagc caggtcgtga cggttaccag tacgctggaa
    36181 gttaccagct ttgtcgccga taaacggacg agcgataaca cgaccgatgt tgtagccgcc
    36241 gttggtcagc tcttcacgag cgatttcgca cagttcgtag agtttgtcca gaccgaaagt
    36301 ttcttcatgg caggcaatct ggaacacgga gtcagcggag gtatagaaaa tcggcttgcc
    36361 ggttttcatg tgctcttcgc ccagctgatc cagaatgacc gtaccggaag agtggcagtt
    36421 accgaggtaa ctcggcagat tagcgcgttc aaccagttta tccagcagct cttgcgggaa
    36481 gctgttttcg tgatcggaga agtatcccca ctcaaacaga accgggacac cggcgatttc
    36541 ccagtgacca gacggggtat ctttaccgga tgacatttcg tgcgcccatg cgtacgcgcc
    36601 gataacttca gcgttgccgt ccattcccgc cggaatgaaa ccggtagaac cttcgtgtgc
    36661 tttcgccagc cccagacggg tcagatttgg cagattgagc gggcctttac gaccgttatc
    36721 agcttcgcct ttggcacaag cttctgcgat atgacccagg gtgtcagccc cgacgtcacc
    36781 aaagcgttct gcatcttctg tagcgccgat gccgaatgag tccagcacca taataaatgc
    36841 acgtttcata tgttctccgt actcagtgct tcaaatttaa atgatcggat cagtataccg
    36901 ttattcgctg atacggcgat agacagttgg tgtgctttcc ggtgctttat cggcaagttt
    36961 aattgccgct ttcaccgctt tcgccgcttc ctgccagctg ttttcgtctt tcgcgtggat
    37021 aactgccagc ggacgctgac cgtctacctg gtcgcccaga cgcgccatat cagtaaagcc
    37081 gacgctgtaa tcgatggtgt cagatgcctg acggcgtccg ccgcccattg caaccactgc
    37141 catccccagc gcgcgggtat ccatttcact gacaaaccct tcggtatcag catagactgc
    37201 tttcgtcagc atcgctgtcg gcagatactt cgcgtagttc tcaacgaagt cggtcgggcc
    37261 tttttgtgcc gctaccatac gaccaaagac ttctgccgct ttaccgttgt ccagcaccgc
    37321 ctgtaatttc gcgcgcgctt cggcgtcatc tttcgccagt ttgccggaga tcagcatctc
    37381 cacgcacagt gccatcgtga catcaaacag acgcgggtta cgatattcac ccgtcaggaa
    37441 ctgcaccgct tcacgaactt caaccgcgtt acctgcactg gaggccagta cctgattcat
    37501 atcggtgagc agcgcggtgg tgcgcacgcc agcgccgtta gccacgccaa caatcgcttc
    37561 ggcaagggct tcagagagtt cgtaggtcgg cataaacgcg ccgctaccca ctttcacgtc
    37621 catcaccagc gcgtccagac cttccgccag tttcttcgcc agaatagagg cggtgatcag
    37681 cgggattgaa tccacggttg cggtaatatc gcgggtcgcg tagaaacgtt tatccgccgg
    37741 agccaatgag ctggtctggc cgataatcgc cacgccgacg tctttaataa tttcgcggaa
    37801 acggttgtca tccgggaaaa tgtcgaagcc agggatggat tccagtttgt cgagcgtacc
    37861 gccagtatga ccgaggccgc gaccggagat catcggaata tagccgccac aggcagcgac
    37921 catcggcccc aacatcagcg aagtcacatc gccgacaccg ccggtcgagt gtttatcaac
    37981 aatcgggcca ttcagatgca ggcttttcca gtcgagaacg gttcctgaat ctcgcatcgc
    38041 catggtcagc gagacacgct caggcattgt catatcgtgg aagaaaatgg tcatcgcgag
    38101 ggcggcaatc tgcccttcgg agatagtgtt gtcgcgaatg ccgttgataa agaaacgaat
    38161 ttcttcatca ctcagcgcat gaccatcacg ttttttacga ataatttctt gtgcgagaaa
    38221 caaggtaacc tcccgtggaa aagagacgag gtaatcgggg aaccggatga gcatcgctgc
    38281 atcatccggc aaatcaagcc ttagtagctg ctggcgctct taccgtcgcc gtggcccaga
    38341 gctttcagca ggcttgccag caagctggaa gcaccaaagc ggtagtgacg cgcatcagcc
    38401 cagtcagcac cgaacagttc atctgcaatg gcgagatatt tctgcgcatc ttccgcagta
    38461 cgcacgccgc ccgccggttt gaaaccaacg gttttttcta cgcccatatc gcggatcact
    38521 tccatcatga tgcgcgcgct ttccggcgtc gcattcacag ccactttacc ggtagaggtt
    38581 ttgatgaagt cagcacccgc tttgatggag atttcagacg ctttacggat cagcgcttcg
    38641 tctttcagtt cgccggtttc aataatcact ttcagcagta cgttcgccgc agcgcaagcc
    38701 tttttacagg ctttcaccag gtcaaaacca acctgctcgt tacccgccat cagcgcgcgg
    38761 tacgggaaca ccacgtcaac ttcatcggca ccgtaggcga ttgccgcacg ggtttctgcc
    38821 agcgcaatgt cgatgtcgtc gttaccgtgt gggaagttag ttaccgtagc gatacggatt
    38881 tccggggtgc cctgctcttt cagagttttg cgagcaatcg ggataaagcg aggatagata
    38941 cagatagcgg cggtattgcc gaccggagtt ttggcctgat gacacagggc gatcactttc
    39001 tcatcggtgt cgtcgtcatt cagggtggtc aggtccatca atttcagtgc acgcaggctg
    39061 cttgctttca gatcagtcat ttcattctcc tggcttgtcg ccaataaaat tcaccttgcg
    39121 agtttgttag tattctaaca tctactccgc aacacacttc gatgtacatc acaattaagg
    39181 aaatctactt acaagtttgc atcactgtaa tgcgatctgg ttcaaataat tcactttcaa
    39241 atgaatgcgt cagtggtggc aaacgcatca ggatcaaagt gaacatcacg aaacttctta
    39301 caatggcgca tcaccttcgg cataaaagga aaagaagatg cccacttctc atgaaaatgc
    39361 actgcaacaa cgttgccagc aaattgtcac cagcccggta cttagcccgg agcagaagcg
    39421 ccattttctg gcactggaag cagaaaacaa tctgccttac cccgcgttgc ctgccgaagc
    39481 ccgccgcgcg ctggatgagg gggtaatctg cgatatgttt gaaggtcatg cgccgtacaa
    39541 accgcgctat gtcttacccg attacgcccg ttttctggcg cacggttccg aatggctgga
    39601 gctggaaggc gcgaaagacc ttgatgacgc actctctctg ctgaccattc tttaccacca
    39661 tgtaccgtcg gtcacatcga tgccggtcta cctggggcaa ctggatgcgt tgttgcaacc
    39721 gtatgttaga attctaacac aagacgagat cgatattcga ataaaacgtt tctggcgtta
    39781 cctcgacaga accctgccag acgcctttat gcatgctaat atcggcccgt ctgattcgcc
    39841 catcacccgt gcgatattac gtgcagacgc agagctgaag caggtttcac cgaacctgac
    39901 ctttatctac gatcctgata tcacccctga tgacctgctg ctggaagtgg cgaaaaacat
    39961 ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa ttttcacaaa
    40021 agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg gtggcagcac
    40081 gctggtacgc ctaaacctga aagccattgc cgagcgcagt gaatcgctgg aggacttctt
    40141 tacgcgcact ctaccgcact actgccaaca gcagatcgcc atcatcgatg cgcggtgtga
    40201 attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag aagggctgat
    40261 taaccctgaa cgttttgtgc cgatgtttgg catgtatggg ctggcggaag cggttaactt
    40321 gctgtgtgaa aaagaaggga ttgccgcacg ctacggtaaa gaggccgccg caaatgaagt
    40381 gggttatcgc attagcgcgc aactggcgga gtttgtcgcc aatacccccg tgaaatatgg
    40441 ctggcaaaaa cgcgccatgt tacacgcaca gtcggggata agttccgata tcggcaccac
    40501 gccgggcgcg cgtttaccat atggcgatga gccagatccg attacccatc tgcaaactgt
    40561 cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc tcgacgaaac
    40621 catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta aagccggaat
    40681 gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg gttatatggt
    40741 gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca ctacctggct
    40801 gggagaagaa gccgcacgca acactcgtat tctggaacgc cagccgcgcg tgataagcca
    40861 tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt gacgggccag
    40921 gcagtcgtct ggctctgttt ttgcagggct gcaatctgcg ctgcaaaaac tgtcacaatc
    40981 cgtggacgat ggggcgctgt aatgattgcg gagagtgcgt gccgcagtgt ccgcatcagg
    41041 cgttgcagat tgtcgacggc aaagtggcgt ggagcgcggc tgcctgcgag caatgtgata
    41101 cctgcctgaa gatgtgtccg caacatgcta cgccaatggt gcaatccatg agcgtggacg
    41161 aggtgcttaa ccatgtccgc aaagcggttc tgtttattga agggataacg gtaagcggcg
    41221 gcgaagccac gacccagttg ccgtttgtgg tggcgctgtt tactgccatc aaaaacgatc
    41281 cgcaactgtg ccatctcacc tgcctcgtgg acagtaacgg catgttgagc gaaaccggct
    41341 gggaaaaatt gctcccggtg tgtgacggcg caatgctcga tctcaaagcg tgggggagcg
    41401 aatgtcatca gcaattaacc ggacgcgata atcagcagat caagcgcagc atctgtttgc
    41461 tggcagagcg cggtaagctg gcggaactgc gtttgctggt aatccctggc caggtggatt
    41521 atttgcaaca tatcgaagaa ctggcggcgt taatcaaggg acttggcgat gttccggtac
    41581 gcctgaacgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg ccgagcgcca
    41641 cgccggagga tgtggaacaa ctggctgatg cgttacggga gcgcggggtg agccggttaa
    41701 tatttccggc gctatatttg tgacatgccg gatgtggcct acggatcggc acagacctgt
    41761 aggcatgata agacgcgtca agcgtcgcat caggcattgt gcgccagctg ccggatgcgg
    41821 cgtgaacgcc ttatccggcc tacggttcgg cacagacttg taggcatgat aagacgcgcc
    41881 agcgtcgcat caggcattgt gcgccaacgg ccggatgcgg cgtaaacgcc ttgtccggcc
    41941 tacggcacgt taaacaacgt ataggtgtta ttaagcaaca cttcggcaat ctcatccgcc
    42001 ggttccggtc gtaactcgca aagcacggca aacacacggg cggcctgttc agggcgatta
    42061 ggctgcccct gaaaaccatt gagcggcata tctggtgcgt cggtttccag caacaacgat
    42121 gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata ggtgatagtc
    42181 ccgcctacgc caattttgta gcccagttgc acaaaccgtt cggcctgttg caggctgccg
    42241 gaaaaaccat gaaccacgcc agtgcacggt aaatcgtggc gtttaagatg catcgccagt
    42301 ttgtcgtgcg tgcgtcgtga atgcaggatc accggcagat cgtagcgttt cgccagtttc
    42361 agttgttcgt cgagtaacca ctgctgcctc tcaaattgcg gatcgtcgcc aaagagatcc
    42421 agaccgattt cccccaccgc caccaccttc gccggatgcc tttccagcgc ctgctgtagc
    42481 tgatcaagag acacatcgct atgtttttcc aacataccag gatgcaagcc caatgcggca
    42541 tacagcggtt gatatttttc cgctaacgcc tgcacgcggg caaaattttc cgcctctgtc
    42601 gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct ggcctcttca
    42661 tcgccactaa acggcgggaa atcaaaatgg cagtgggtgt cgataaaacg gcaaatcaag
    42721 ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcgctc acttcggcaa
    42781 ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgactccggc gtcaccaccg
    42841 gcaccagatg gcggctcagc ggcgcttttt cagtcaataa tttaccgacc gttgccagaa
    42901 aataacgccc acataaacgc ccaagtttat agtcttcacg cagcgccgga atccgactgc
    42961 cgagcgcgat actatgcaat ggcttcggcg gataaatttc gaatatccgc agctggcccg
    43021 gtggtttctc aataaattgc tgaatctcac gatagctggt ttcatgatgc tgcaccagat
    43081 tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg aaccactgtg
    43141 gcgtgtagta catttgtgac ggcacagtgc ggatgacgac caacgttttt gctccctgcc
    43201 ttgccgcctc tttaaccggg atcgcatcac tgatcccacc atccaggtag ttaatacctt
    43261 ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga atcacatcca
    43321 gccagttttg tttagttggc aaaaagtaat tcggcgcata gtcatcctga cgacaggcgc
    43381 acatataaaa cgatttgccg ctgtcaaata accgcgctgc ggtgtccatt tgcaacggca
    43441 tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct ccacggacaa
    43501 agcgcaatgg atcgaaaaat tcgcgttttg tggtgtaacg catgatgact ttgcgcgcgt
    43561 aaccgggctg attgcagata tacgccgaga ggttctgcgc cccggcagat gtgccgagat
    43621 aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca gccgtgaaaa
    43681 ttccacgttg tccgccgcct tcgcacacca gagcaatccg cccaggctgg aacggcctta
    43741 gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc tctgccttcc
    43801 tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa aaccgcaaaa
    43861 gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat ctagggtcgt
    43921 tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa gacaatttta
    43981 gctgcgcctg cagcagtacc ggccagacca ccaaacccaa gtgcggcggc gattaacgcg
    44041 ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat agttaatgtc
    44101 accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg tgcacatcgc
    44161 gcctcccgac aaagttcggg aggtcgaatt acgacgaatt acttagtttt cagatcattt
    44221 ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc gctctgtgcc
    44281 tgagaatcta cggtaccgga gagctggacc acgccatcgg tggtttcaac tttcacatga
    44341 cgggaaggga caatgtcatc tgccagcagt ttggctttga tttcactggt agtggcggtg
    44401 tcacccgcgt agcctttcac agagccttct ttagcgtcgc gaacgtgcag tttgtcgctg
    44461 acagaggtca cgccctcaac acctttcgcc actttcaccg cctcttcggc ctgagcctgg
    44521 ctttcaacga aaccgctcag ggtcacgact ttttgatcgg ttttgacaga gatatcggtg
    44581 ctcttgatgt tgtcatgatc caccagggcc gccttcactt tcgcggtgat ggcgctgtca
    44641 tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc gctttcattg
    44701 gtagtctgcg cgttgttttc tgcgtaggca gagccggtcg cgacggcaga ggtcaacatt
    44761 acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt attcctgtat
    44821 gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg gagagacttc
    44881 accctctaca gaagtcaatg gtcgccatca caaaagcgat gggtgatgaa taacgaccat
    44941 tacagcctct gaatcagtta ttaatatcgg tagaatgaca atagacggct ttagatactg
    45001 atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt gttaaatata
    45061 gatcacaatt ttgaaaccgc tcgggatatc agctaaaaca taagcaaaag tgaatgtttt
    45121 aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag cggctaccgc
    45181 gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcc ggataacgtt
    45241 cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta tcgccgccat
    45301 caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc gcgtccgcac
    45361 attctaccca gcgggcagtg gcaacgttga ctgactcata cactgcttca acgttgtatt
    45421 cgctcttcag gcgcgctacc accacatcaa actgcagcac accaaccgcg ccaacgatca
    45481 aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa agctgtacca
    45541 gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga cggaacagtt
    45601 ctggcgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta aaggtgtcgc
    45661 cgatctgaat ggtgccgtgg ttgtgcagac caaggatatc gccaggatac gcttcttcaa
    45721 cgtgcgaacg gtcacccgcc ataaaggtca gcgcgtcgga gatcaccaca tctttcgcgg
    45781 tgcgcacctg gcgcagcttc atgccttttt catatttacc ggataccacg cgcataaacg
    45841 ccacgcggtc gcggtgtttc gggtccatgt tggcctgaat tttaaatacg aagccggtaa
    45901 atttatcttc gctcgcttct acggtacggg tatcggtctg acgcggcatc ggcgcaggcg
    45961 cccactccac caggccatcc agcatatgat cgacgccgaa gttacccagc gcagtaccaa
    46021 agaataccgg ggtaatttcg cccgcaagga acagctcttt gtcgaactca ttagacgcgc
    46081 ctttcaccag ctccagttcg tcacgcagct gctgtgccag atcttcacca accgcagcgt
    46141 cgagatccgg gttattcagc cctttaacaa tgcggacttc ctgaatggtg tggcctttac
    46201 cgctctgata gagataggtt tcatctttat aaaggtggta aacgccttta aacagcttgc
    46261 cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc tcaacttcat
    46321 cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag gtgaggatcg
    46381 gcgtatcgcg cagacgggta acttccatca gcttacgggt acgatcttct acaccttttg
    46441 cggcgtcgat aaccatcagg cagcagtcca ccgccgtcag ggtacgatag gtatcttccg
    46501 agaagtcttc gtgccccggg gtatcgagca agttaaccag gcagtcgtga tacggaaact
    46561 gcatcacaga cgtagtgatg gagatcccac gctgcttttc catctccatc cagtccgact
    46621 tagcgtgctg gttagagcca cggcctttta ctgtaccggc ggtctgaatg gcctgtccga
    46681 acagcagcac cttctcggtg atggtagtct taccggcgtc cgggtgagaa ataattgcaa
    46741 aggttcttct tttcgctacc tctgcggcca aaaggcttgt cataattgtt gtctctttga
    46801 ttatgtacaa tcatatgtac aaagaaaaat tcatgccgct gattgtacat tttggaggta
    46861 tcagagcata gcagaacatg atcagtaggt gtatttgtag ccaaagaaca tttgtacatg
    46921 cagcaatctt tgctctattg acactgttta tttatacagt taaagataat gctgtatgca
    46981 aacacagtgt agagggagtt ttatgcgtat tgagatctgc atagccaaag agaaaatgac
    47041 taaaatgcca aacggtgctg tggatgcgtt aaaggaagaa ttaacccgac gcatcagtaa
    47101 gctttatgac gatgtggatg tgatcgtaaa atctgccagc aatgatggac tgacaatttt
    47161 aagatccgtg gataaagagt ctgctcaaaa gttcgttcag gaaacgctgc aggaagtctg
    47221 ggaaaccgcg gacgactggt ttattcggta aatcttcttt acggggagtt aacttactcc
    47281 ccatttttta ttgcatgata attgcagcaa cggcacagag tgtcaccaca ccacccagcc
    47341 acacaaacag catgaagcac attgctcccg ctgtataacg ttttctcccg tacatacggc
    47401 atttgtcttt attcatcatc tgcccctgaa tcacctgaag acgctgcagg tccggtaaca
    47461 ctgaatcact ccgcacacgc aagatgtttc acgctcacac catacagccc gtttttcgcc
    47521 attcccgtat acagaaaggt attcaccgtc ttcgtgtgaa tacccatttc actggcaagg
    47581 cgacagggtc tcgactggcg gtagacgtag ccaaacatca ccaccatctc tgacatcgtc
    47641 agcggacggg caacgctgtt atacgtccat acaccattgc gtatggcatc acttatctgc
    47701 tcaccatcac ggagtgattc cgcacccagt tgcagaaaat gccgtccctg gaacatgcag
    47761 cgcatcagtc tgcccagcaa cgtatcacag attatcagcc aggattcgtc aaagtccggg
    47821 atataacggc ggttctccgg caggaaaaac tccctcgcca gtatcacact gtgttccttc
    47881 agtaacgcct gcagaccata ctccagatag cgattcctgg tagccagaat caccttcatc
    47941 gcagcacctt cctgtttttt atgcctcatg aaaaaaacgg caggcaaaac tcctgtgcct
    48001 gccgtcatgc ttcatgtcac tgtcagcaat taaaactcga agttcacacc tacgttgtag
    48061 gaaacatcac cgtcgctggc tgcaatacct gctttggctg c
//
LOCUS       DAZGJT010000027        46191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZGJT010000027 DAZGJT010000000
VERSION     DAZGJT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 46191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.027"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..728)
                     /locus_tag="ACHKFK_001687"
     CDS             complement(<1..728)
                     /locus_tag="ACHKFK_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO9052048.1"
                     /translation="MIIEKVMNNNCVQASMNGQEVIISGPGVGYNKKYGMSVPEHPAN
                     RIFYVRNEQKNKLYKLIEHVDIEYVFVAEKIVQYAEKNLEKNLNPSLLLILADHISNA
                     ISRVVSGIQINNVFLDEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILN
                     NYENSVDYESVRIIELSQIITELIEVVYNRKVDRSSFNYSRFMMHLKYFSSRVLCNEK
                     IKQKDIGDIYEQFLEKDILLQRAI"
     gene            complement(985..2238)
                     /gene="amiC"
                     /locus_tag="ACHKFK_001688"
     CDS             complement(985..2238)
                     /gene="amiC"
                     /locus_tag="ACHKFK_001688"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO9052049.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLTRRG"
     gene            2470..3801
                     /gene="argA"
                     /locus_tag="ACHKFK_001689"
     CDS             2470..3801
                     /gene="argA"
                     /locus_tag="ACHKFK_001689"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO9052050.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAQTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(3863..5689)
                     /gene="recD"
                     /locus_tag="ACHKFK_001690"
     CDS             complement(3863..5689)
                     /gene="recD"
                     /locus_tag="ACHKFK_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO9052051.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     SHVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(5689..9231)
                     /gene="recB"
                     /locus_tag="ACHKFK_001691"
     CDS             complement(5689..9231)
                     /gene="recB"
                     /locus_tag="ACHKFK_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO9052052.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYARLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLTREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDAVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWQMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCNDDIAWQTAQIGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFEPQWEPVLTEWITTVLLAPLNETGISLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYATRPNAGLIDLMDEMFASMTLEEA"
     gene            complement(9224..12112)
                     /gene="ptrA"
                     /locus_tag="ACHKFK_001692"
     CDS             complement(9224..12112)
                     /gene="ptrA"
                     /locus_tag="ACHKFK_001692"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO9052053.1"
                     /translation="MPRSIWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQEG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQKKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARHVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAIFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRTMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(12288..15656)
                     /gene="recC"
                     /locus_tag="ACHKFK_001693"
     CDS             complement(12288..15656)
                     /gene="recC"
                     /locus_tag="ACHKFK_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO9052054.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTDELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLSS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCHHTRMPFDPQNYQPGN
                     LQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNVLVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(15669..15992)
                     /gene="ppdC"
                     /locus_tag="ACHKFK_001694"
     CDS             complement(15669..15992)
                     /gene="ppdC"
                     /locus_tag="ACHKFK_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9052055.1"
                     /translation="MSASLRNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(15977..16384)
                     /gene="ygdB"
                     /locus_tag="ACHKFK_001695"
     CDS             complement(15977..16384)
                     /gene="ygdB"
                     /locus_tag="ACHKFK_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO9052056.1"
                     /translation="MNREKGVSSLALVLMLLILGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALEWGKMHSWQTQPAVQCLLYAATGARVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(16381..16944)
                     /gene="ppdB"
                     /locus_tag="ACHKFK_001696"
     CDS             complement(16381..16944)
                     /gene="ppdB"
                     /locus_tag="ACHKFK_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9052057.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTNTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCVIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMHAASKADPQTVVDASYSVTGSNL"
     gene            complement(16935..17405)
                     /gene="ppdA"
                     /locus_tag="ACHKFK_001697"
     CDS             complement(16935..17405)
                     /gene="ppdA"
                     /locus_tag="ACHKFK_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO9052058.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISLIREGTLWCLVSSVAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(17590..18384)
                     /gene="thyA"
                     /locus_tag="ACHKFK_001698"
     CDS             complement(17590..18384)
                     /gene="thyA"
                     /locus_tag="ACHKFK_001698"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO9052059.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNISSYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(18391..19266)
                     /gene="lgt"
                     /locus_tag="ACHKFK_001699"
     CDS             complement(18391..19266)
                     /gene="lgt"
                     /locus_tag="ACHKFK_001699"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO9052060.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(19417..21663)
                     /gene="ptsP"
                     /locus_tag="ACHKFK_001700"
     CDS             complement(19417..21663)
                     /gene="ptsP"
                     /locus_tag="ACHKFK_001700"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9052061.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(21676..22206)
                     /gene="rppH"
                     /locus_tag="ACHKFK_001701"
     CDS             complement(21676..22206)
                     /gene="rppH"
                     /locus_tag="ACHKFK_001701"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO9052062.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(22559..22705)
                     /gene="ygdT"
                     /locus_tag="ACHKFK_001702"
     CDS             complement(22559..22705)
                     /gene="ygdT"
                     /locus_tag="ACHKFK_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO9052063.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            22891..23580
                     /gene="mutH"
                     /locus_tag="ACHKFK_001703"
     CDS             22891..23580
                     /gene="mutH"
                     /locus_tag="ACHKFK_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO9052064.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAKRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQIERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            23649..24362
                     /gene="ygdQ"
                     /locus_tag="ACHKFK_001704"
     CDS             23649..24362
                     /gene="ygdQ"
                     /locus_tag="ACHKFK_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO9052065.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            24500..24718
                     /gene="ygdR"
                     /locus_tag="ACHKFK_001705"
     CDS             24500..24718
                     /gene="ygdR"
                     /locus_tag="ACHKFK_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO9052066.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            24826..25866
                     /gene="tas"
                     /locus_tag="ACHKFK_001706"
     CDS             24826..25866
                     /gene="tas"
                     /locus_tag="ACHKFK_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO9052067.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMEQLKTNVESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25898..27091)
                     /gene="lplT"
                     /locus_tag="ACHKFK_001707"
     CDS             complement(25898..27091)
                     /gene="lplT"
                     /locus_tag="ACHKFK_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO9052068.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHILVALAVCALAYGGAVVANIYIPKLAAARPGQSWNLISMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(27084..29243)
                     /gene="aas"
                     /locus_tag="ACHKFK_001708"
     CDS             complement(27084..29243)
                     /gene="aas"
                     /locus_tag="ACHKFK_001708"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO9052069.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTKALKGERVLITPNHVSFIDGIL
                     LALFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            29829..30860
                     /gene="galR"
                     /locus_tag="ACHKFK_001709"
     CDS             29829..30860
                     /gene="galR"
                     /locus_tag="ACHKFK_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO9052070.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETIGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(30867..32129)
                     /gene="lysA"
                     /locus_tag="ACHKFK_001710"
     CDS             complement(30867..32129)
                     /gene="lysA"
                     /locus_tag="ACHKFK_001710"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO9052071.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDMVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVLEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     AGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAAEGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            32251..33186
                     /gene="lysR"
                     /locus_tag="ACHKFK_001711"
     CDS             32251..33186
                     /gene="lysR"
                     /locus_tag="ACHKFK_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO9052072.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERIRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPRLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFHSENYISLSRTDSYRQLLDQLFTENQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(33173..33865)
                     /gene="ygeA"
                     /locus_tag="ACHKFK_001712"
     CDS             complement(33173..33865)
                     /gene="ygeA"
                     /locus_tag="ACHKFK_001712"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO9052073.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCSLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33994..35412)
                     /gene="araE"
                     /locus_tag="ACHKFK_001713"
     CDS             complement(33994..35412)
                     /gene="araE"
                     /locus_tag="ACHKFK_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO9052074.1"
                     /translation="MVTINTESALTPRPLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(35727..36488)
                     /gene="kduD"
                     /locus_tag="ACHKFK_001714"
     CDS             complement(35727..36488)
                     /gene="kduD"
                     /locus_tag="ACHKFK_001714"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO9052075.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSETDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(36518..37354)
                     /gene="kduI"
                     /locus_tag="ACHKFK_001715"
     CDS             complement(36518..37354)
                     /gene="kduI"
                     /locus_tag="ACHKFK_001715"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO9052076.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPVTKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(37641..38822)
                     /gene="yqeF"
                     /locus_tag="ACHKFK_001716"
     CDS             complement(37641..38822)
                     /gene="yqeF"
                     /locus_tag="ACHKFK_001716"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO9052077.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVITQSNGQTLVVDTDEQ
                     PRTDTSAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            39077..40306
                     /gene="yqeG"
                     /locus_tag="ACHKFK_001717"
     CDS             39077..40306
                     /gene="yqeG"
                     /locus_tag="ACHKFK_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9052078.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTKQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWKSIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            complement(40623..40696)
                     /locus_tag="ACHKFK_001718"
     tRNA            complement(40623..40696)
                     /locus_tag="ACHKFK_001718"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40662..40664),aa:Gly,seq:ccc)
     gene            complement(40775..41530)
                     /gene="actS"
                     /locus_tag="ACHKFK_001719"
     CDS             complement(40775..41530)
                     /gene="actS"
                     /locus_tag="ACHKFK_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO9052079.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            41944..44221
                     /gene="xdhA"
                     /locus_tag="ACHKFK_001720"
                     /pseudo
     CDS             41944..44221
                     /gene="xdhA"
                     /locus_tag="ACHKFK_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            44232..45110
                     /gene="xdhB"
                     /locus_tag="ACHKFK_001721"
     CDS             44232..45110
                     /gene="xdhB"
                     /locus_tag="ACHKFK_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO9052080.1"
                     /translation="MFDFASYHRATTLADAITLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITQAEDGALRIGSATTFTQLIEDPVIQRNLPALCAAAASIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            45107..45586
                     /gene="xdhC"
                     /locus_tag="ACHKFK_001722"
     CDS             45107..45586
                     /gene="xdhC"
                     /locus_tag="ACHKFK_001722"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO9052081.1"
                     /translation="MNHSETITIECVINGMPFQLHAAPGMPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLFLAAWAEGKEIRTLEGEAKGGKLSHVQLAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(45626..>46191)
                     /locus_tag="ACHKFK_001723"
     CDS             complement(45626..>46191)
                     /locus_tag="ACHKFK_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9052082.1"
                     /translation="DIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQE
                     DIELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSG
                     EVIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNS
                     KKEVADELGIGIATLYRKIKKYELLNT"
BASE COUNT        11566 a        12366 c        11177 g        11082 t
ORIGIN      
        1 tgaattgcgc gctgtaacag gatgtccttt tcaagaaact gttcatagat atcaccaata
       61 tctttctgtt ttattttttc attgcataac acgcgacttg aaaaatattt aaggtgcatc
      121 ataaatctgg aatagttaaa tgaacttctg tccacctttc tgttataaac aacttcaata
      181 agctccgtga ttatttgcga caactcaata atccgtactg attcataatc aactgaattt
      241 tcataattat ttaaaatatg caatgctata aaaccaatct catcatcagg aagttgaacg
      301 ctgaattgct cattaatgat agttaatgca tcgcgactta tcgcatactc tgctttgtac
      361 aacgctttga tttcatcaag gaaaacatta ttaatttgta tacctgaaac gactcgggat
      421 attgcattcg aaatgtgatc cgcaagaatc aatagtagcg atggattgag atttttttcg
      481 agatttttct ccgcatattg cactattttt tcggcaacaa atacatactc aatatctaca
      541 tgttcaatca atttataaag tttgtttttt tgttcatttc tgacataaaa aatccggtta
      601 gccggatgct cagggaccga cattccatat tttttgttgt aaccgacgcc aggcccagaa
      661 atgataacct cctgtccatt catcgatgcc tgtacacaat tattgttcat gactttttcg
      721 atgatcattc catggctcca aaaaaaaagc aaggcacgtc agaagtaata actacttcaa
      781 acattgcctt gcctgattta acagttacaa gatgattaac taatgcgtat acattacacg
      841 gaattgtgct taattaaaga gatggtcggc ttatttttca agccgacatt caaatatccg
      901 acatcagtca caaaaccagc gtctaatatt caggtaaatg ttatctattc aacgaaatat
      961 aaattcattg aataaataaa aacattatcc ccttctcgtc agcgtcgccc catcggcaaa
     1021 atacgcttta attcccgcaa gaatagactc cgcaacttcc tgctggaaag tcgccgtttt
     1081 cagtttacgc tcttcctcaa cgttactgat aaacgccgtt tcgaccagaa tggagggaat
     1141 atctggtgcc tttagtacgg caaacccggc ctgttcaact tgatttttat gcagcttgtt
     1201 gattttaccg agcttattca gcaccgcttt accaaactta aggctgtcgg caatggtcag
     1261 cgactgcacc atatcgaaca tggtgtggtc gacatagcgg tcaccgcttt tgcttacgcc
     1321 accaatcaag tccgaggcgt tctgggtttg tgccagatat tttgccgcag tactggttgc
     1381 gcctttggtt gagagcgcaa acaccgagga accgctcggc tggcgactgg taaaggcgtc
     1441 ggcatggata gaaacaaaca agtcagcgcg ctgtttctgg gcttttgcta cgcgcacttg
     1501 caacggaatg aagatgtctt cattgcgcgt catgtacacc ttcatattgc cctctttctc
     1561 gatcagagag cgcagacggc gagctatttg caataccacg tctttttcgc gcgttttgta
     1621 tttccccacc gcaccggagt cttcgccacc gtggccaggg tcaagcataa tgacaatcgg
     1681 acgatcccgc ccagctttac ccggttgtgg accactttgt gctggcggca cctgcttttc
     1741 gaggtcgcct ttgttgtaat cctccagcag cgccagcagc gggtcctgca tatcctgtgc
     1801 attggccgga tagaggtcca tcaccagacg ctctttaaac ccggcgaccg gcgcaagggc
     1861 aaacagctgc ggttttacgt tttgctttaa ttcaaaaacc atacgtacgg tttgcgggtc
     1921 aaattgcccg acgcgcgccg acttgatgaa cgggtcgtca gcgcggattt gcgcagccat
     1981 ccccttgagc accgagttca ggtttacatc ttcgatatcc accaccacgc gttcaggatt
     2041 actcaacgcg aactgcttat atttcagctg acgatttgat tctaccgtca cgcgggtgta
     2101 gctggacgca ggccagacgc gcaccgccac gacctggctg accgcagcca ggctgacctg
     2161 acttacgctc aatagccaca tggcacccgc gccttgcagt aaacgacgac ggctgattgc
     2221 agtgttggat cctgacatgc ctctcccgag caaaagaaat ctaagctgtg taacaagtaa
     2281 acgaccaatt tgaccggaaa ctttagcgaa agacgcataa tctgtcatct ataaaacggt
     2341 aaacattctt tttatattca cggtattact gataaaaaac tcgctcccgc ataaaattta
     2401 cacttgcacc ccgcgaaaaa acagaataaa aatacaataa tttcgaataa tcatgcaaag
     2461 aggtgtaccg tggtaaagga acgtaaaacc gagctggtcg agggattccg ccattcggtt
     2521 ccctatatca atacccaccg gggaaaaacg tttgtcatca tgctcggcgg tgaagccatt
     2581 gagcatgaga atttctccag tatcgttaat gatatcgggt tgttgcacag cctcggcatc
     2641 cgtctggtgg tggtctatgg cgcacgtccg cagatcgacg caaatctggc tgcacatcac
     2701 cacgaaccgc tgtatcacaa gaatatacgt gtgaccgacg cccaaacgct ggaactggtg
     2761 aagcaggctg cgggaacatt gcaactggat attactgctc gcctgtcgat gagtctcaat
     2821 aacacaccgc tgcagggcgc gcatatcaac gtcgtcagtg gtaattttat tattgcccag
     2881 ccgctgggcg tcgatgacgg cgtggattac tgtcatagcg ggcgtatccg gcggattgat
     2941 gaagacgcga tccatcgtca actggacagc ggtgcaatag tgctaatggg gccggtcgct
     3001 gtttcggtca ctggcgagag ctttaatctg acctcggaag agattgccac tcaactggcc
     3061 atcaaactga aagctgaaaa aatgattggt ttttgctctt cccagggcgt cactaatgac
     3121 gacggtgata ttgtctccga acttttccct aacgaagcgc aagcacgggt agaagcccag
     3181 gaagagaaag gcgattacaa ctccggtacg gtgcgctttt tgcgtggcgc agtgaaagcc
     3241 tgccgcagcg gcgtgcgtcg ctgtcattta atcagttatc aggaagatgg cgcgctgttg
     3301 caagagttgt tctcacgcga cggtatcggt acgcagattg tgatggaaag cgccgagcag
     3361 attcgtcgcg caacaatcaa cgatattggc ggtattctgg agttgattcg cccgctggag
     3421 caacaaggta ttctggtacg ccgttctcgc gagcagctgg agatggaaat cgacaaattc
     3481 accattattc agcgcgataa cacgactatt gcctgcgccg cgctctatcc gttcccggaa
     3541 gagaagattg gggaaatggc ctgtgtggca gttcacccgg attaccgcag ttcatcacgg
     3601 ggcgaggttc tgctggaacg cattgccgct caggcgaagc agagcggctt aagcaaattg
     3661 tttgtgctga ccacgcgcag tattcactgg ttccaggaac gtggatttac cccagtggat
     3721 attgatttac tgcccgagag caaaaagcag ttgtacaact accagcgtaa atccaaagtt
     3781 ttgatggcgg atttagggta agcgatgaaa atcgccggat gcgacatgcg taacactcgt
     3841 gcgtcgcatc aggcaattac gtttatcccc gtgaactaaa caacgccgcc agaccactgc
     3901 gccgctcagt acgagtggcg attgccgcac ttaatatgcg ctcatcggca tacagcgaca
     3961 gacggcgacg cgcgcgggtc accgcggtgt aaaccagctc tcgcgttact accggcgtgc
     4021 gttgactcgg taaaatcaac gccgcatggt cgaactccga tccctgcgat ttatgtaccg
     4081 tcatcgccca cgtcgtttcg tgctctggca ggcgactcgg ttgcacagac ttaatattgc
     4141 cgtccggcat cgcaaaccag acgcgcgtcc cctgcccgcg atccagcgca ataccaatat
     4201 cgccattaaa caacccaagc gcgctgtcat tacgggcaat catcaccggt cggccttcgt
     4261 accaacgaga gtgcggatga cgatgaattt tgcgcttctg ttgcataaac tgctcaattc
     4321 gctcattcag tccagccacg ccaaacggcc cttcccgcag ggcgcacaaa agctggtact
     4381 cattgaacgc ctgaatgatt aaatccggct cggcacgcgc ttgcagcaga tccagataac
     4441 gtccgtaacc cgcaagagct tcctcaagca tcgcaatata atcttcgccg ctctgtaaaa
     4501 gtcgtttttc gatatcagta aaatcctgct gaaaaacggt tttcactgcc gttttatcac
     4561 cacggttaat cgccgcagct aactgaccaa tgccagaatc gctgccgaaa cgatagcttt
     4621 tttgcagcag gcagaggctg tcgcgcaaag atgccgcttc tgtgccagtt cctgctggaa
     4681 cgtggcttcc cgtcaggcgg ctcaactgcc cggcacgctc ggcggtaaag cccgcgttgg
     4741 cataagcgca gatatcgccc agcacagccc cagcctcaac cgaggccagt tgatcacgat
     4801 cgccgagaaa gatcactcgc gcatgatcgg gcaaggcgtc gatcagtctc gacatcatag
     4861 gaagatcgat cattgacgct tcatctacca ccagcacatc aagatgcagc gggttaccgg
     4921 catgatgacg taaacgctgg ctacccggct gcgcacccag caatcggtgc aaagtgctgg
     4981 catcttccgg aatgcgtttc ttttgttcat cggtcagcgg taattgtcgc aaagccttgc
     5041 cgagagattc ggttaagcgc gcggcagctt tacccgttgg tgcagccaga cggatacggc
     5101 agcgttcgcc gtcggccatc tgaattaagg ctgccagcaa cttcgctacg gtggtcgttt
     5161 taccggtgcc aggaccgccg gaaatcaccg aaatccgccg cgtcagcgcc actgccgccg
     5221 caactttttg ccagttaatt tcatcgctta ctggaaaaag tttgtccagg gtttgcgcca
     5281 gtagagcttc atcaacctca atggcatgat tcacttcgtt gaaaaagcgt gccactgtgc
     5341 gctcgttaca ccacatgcga ttcaaataaa gacgatcgcc acagaggatc atcggcgttg
     5401 gttcatctcc ccggctgacc gcttgtgaag ccagcaagca ttcttcccaa ttttgtagtt
     5461 caccgatttc actgacacag gtcgccaaca gcggatgcga tgcctcgtta ttttccagtc
     5521 gtgaaagcgg caaacaaacg tgtccctctc cggcatcatg acttaatagt gccgccgcga
     5581 gggtgacggc aggatgttca tctcccgcca cggtcagggc aaactgcacg tccagcgggc
     5641 gtagctgttt gtgctccaca gcttccagta attgcttttg caatttcatt acgcctcctc
     5701 cagggtcata ctggcaaaca tctcatccat caggtcaatc aacccggcgt tgggtcgggt
     5761 cgcgtagatc ccttgttgcg gatgttcttt atcaacgcca cgcaggaaca gataaataac
     5821 gccgccaaag tggcgctcat agtcgtaatc agcaatacga tggcgcagat aacggtgcag
     5881 cgccagggta taaagctgat attgcagatc atagcggtgt gcctgcattg ccgctgccat
     5941 agcctgttgg gtgtaagccg aactgtcttc acccaaccag ttggatttat agtcgagcag
     6001 gtaataacgc ccttcgtggc ggaacaccag atcgataaag ccttttaaca tgccacgtac
     6061 ctgcatgaac tccagcggcg ggcagccagc ggatagcggg tcaaactggc gaattagtgc
     6121 atcaagctgg ctggcgatga gcggttcgct aattggcagg taaaactcca tctccacctg
     6181 tttatcgcga tcggaaagct gactcaggct aatacccgtt tcattgagag gtgccaggag
     6241 gacagttgtg atccactcag tcaacaccgg ttcccactgc ggttcaaagc caccgagctc
     6301 cagtttttcc tgtacccagt tcgggtcaac cggctgggta aaatcgaggt cttcaaacaa
     6361 actgtgcaag aatgtccccg gtgacgcacc gcgcgggaac tgatgcggtg ttaacgtcgg
     6421 ttcttcaacg acgctgacca cgcctgcggc atcaacatcc agccgtggca tcagatcctg
     6481 agcgataccg tgaccacgct gctgcaaacc ggagtagctg gtgacacgcc agttatcgcc
     6541 gggcaatcgt tgtagcgtcc tcgcattcag ttctgcagtt aatgcatcat taacctgcca
     6601 gggctggtta tcaccaattt gtgccgtttg ccaggcaata tcatcattgc acaacgcttc
     6661 aatacaggta cgaagccctg ccgcatcttg cggttctcct ttttgcagca aacgcccgag
     6721 cgcactttgg tggacgtcgg tatcaccttt tttatcgcca cgacggcgca ccagcggtgc
     6781 aacgccgaga ctgcaatgcc aaactgaacg cgtcagcgcc acgtaaagta aacgcagatc
     6841 ttccgccaga cgttcggcct ccgcgaggtc gacgctttct ggcgcagcat taagatccag
     6901 aacggcctca aacgagtggc gatcgtgata aaacgcctga tcctggacgc ggaaattggt
     6961 aataaatggc aaccagacca gcggatattc cagccctttt gatttgtgta tcgtgacaat
     7021 ctgcaccaga tgtttatcac tttcgaggcg catttgttgg ctggaggcat tactgtctgg
     7081 ctcgaggata tgttgcgata accagcgtac cagcgcatgt tcactttcca gctgcgttcc
     7141 ggcttcttgt agcagttcgc tgatatgcaa gatatcggta agacgccgct caccgcctgc
     7201 cgttgccagc aagttttctg caatgttacg cgccgacatc agcgcccgca gcatcggcat
     7261 aacgccacgt ttgcgccaga tttgccgata accatcgaac tcttcgacga ccgcatccca
     7321 cgcatgttcg tcattattca gcgtttcaat atccagcgca ttcagcccca tcattgacgt
     7381 tgccagcgca ctgcgcaggg tgttctcacg ttcgggcgtc atcaccgcct gcaacagcca
     7441 aagcatctcc tgtgcttcca gagtttcaaa aacactgtcg cggttcgaaa ggtaaacgga
     7501 agggatttcc agcagcgtta aggcatcgcg cacctgagcg gcctcctggc ggctgcgcac
     7561 cagcacactg atgtccgaag cacgcaccgg acgcgcgtcg tcgccgttca tcagcaacgc
     7621 ttcgccgcgt tgcccggctt gtaaccagtc gcggatttgc gcagcacata cctgcgccat
     7681 ggtactttga tagtcgccaa cgccgcagct ttcgccttcc atctgccaca ttttcatcgc
     7741 aggctgcgtt tcacctttaa acacaaaacg taacgcctga tttttgccgg cagatttcac
     7801 aggaataaac ggtatttcac gaaacatgaa cgcgtcatca gtctggctga aaagtttgtt
     7861 aacgctgttc accattcccg gtgcggaacg ccagttggta tctaaggtgt agtgagcatg
     7921 aacttcgcta cgcgccttca tataagtgaa gatatccgca ccccggaatg catatatggc
     7981 ctgtttcggg tcgccaatca gcaacaatgc ggtttccggc tgatggtgcc agatacggcg
     8041 aaaaattcga tactgttggg ggtcggtatc ctgaaattca tcgatcattg ccaccgggaa
     8101 tcgcgtacgg atcgccgctg ccaatacctc gccgctttcg ctacgcagcg cggaatcgag
     8161 ccgacttaac atgtcatcaa aacccaattc gccacggcgg cgtttttcac gcgctactgt
     8221 ttcgcggatc tcagccaatg cgcgggtgat caccagatcg cggatcgaca atggttctgc
     8281 aagcagttga tcgatcgcct caaacagtgg atgtcgcggg gtttcccccc cggctttcgt
     8341 gcgatcttct aagaaacgct gagagaattt ttccagcgac tctggcaact gataactgtt
     8401 ggtctcttct tctgcccagg cgctgatctt gtcgatccat ttagcctgat tgctacggtt
     8461 aaacttgcgt cgatcaatac cagaagattc gatcagcgca tccagttcac ccaccgcgtc
     8521 gcgccactgc tgttttacgg cgtcgatacg cgccacaatt tgcgcgtggc gggaagccag
     8581 cgtttcatca tcgggcggcg gtgctttgat aaccggtgct tcgccttgca gataacgatt
     8641 aatatcgcgc agcaacgcct gcggcccttt ccaggtttca aagaccacct gggcaatttc
     8701 acgcgtcagc gggtagcagt ggcgacgcca gaaatcggcg caggcctggt agcgtagcag
     8761 agactcatct tcaatcagct gctgctcaaa cagcatgccg gattcaaagg cattcaggtt
     8821 gagcatgcgc tggcaaaaac cgtgaatagt aaagactgcc gcttcatcca tctgccgttc
     8881 ggctaacaac aaccactgcg cggcttgcgc tttatcgtcg atctcttcca gcagacgtgc
     8941 atacagcgga ttgtcagtgg tttcacgcag gcaggcgata cgcaactcgt gaatattgct
     9001 acggatacga ccgcgcaatt ctgccgtggc agcctcggta aaggtgacca ccagcagttc
     9061 ttcaacggtc agcgggcggg gaaaggcggc ggaaccgcct agcccaagta acaggcgcaa
     9121 atagagcgct gcaatcgtaa aggttttgcc tgtgccggca gaggcttcaa tcaggcgctc
     9181 accctgtaag ggcaagcgca aaggatctag tgtctcggcg acatcactca ttcttttcac
     9241 tcatcagggg cattgtttgc tgcaacgcgc tgacgttctc ccacactttc cagccttcag
     9301 gatgcacata ttcggctttc ccgttctggc tgccggaaat ctgcgacaga atagccatac
     9361 cttgcggctc gaccaccgcc tgatggaaga aatcagcaag tttttgcggc gtcagcagtt
     9421 ttatctgggc cacgatttta tcacgcgaat cgaagcgcat attgccgcga tcgaaatctt
     9481 tacttaactt cgatgcttct tcgccgagcg tttgcggtgc ctgcagcatc tgggttatta
     9541 ccgcctgctg gatttgcgca aactcttctg gcttcatcgt ccgcaatttt gcctctgcgg
     9601 ttgggaaaaa cgccttgtaa cgctcccaca agaatgaagg ctgtttatca ttgctttgca
     9661 aaaggaagcc catgccccac tgacgcccca cgctcattgg aaacgcaaac acggcatagc
     9721 ccaactgttc ttcggtacgc aactgattgt agaaccacgg ctgtacgatc tgccccaaca
     9781 gagagctata ggctgagctg gtgtattcat cgtagccagt cggtacaaat atcgctgcca
     9841 gtgcggagtc ggtgctgtta ccagcttttt caaagatgac ggattgtttt ttatcgacca
     9901 cgacatcttt gttacgacac cactctgaac catcagcgcc caactgtttt tgcacatggc
     9961 gtgccagcgt tgttgcctgg gcttcagtca tgttgccgat aaccataaac tctggtctag
    10021 cccctgattt taaggcgtcg cgataggcca gcacctcttt caacgtaatg gagggcaaaa
    10081 ttttgcgccg ttcatctcgc gagaagtacg gcacttgcga gagcatctgc gcgggcataa
    10141 tcgcctgctc aaacgctttg cccttttctg cggaatccat catctggtta taccaggact
    10201 tcgcctgctc aagctgatct tccgtagcgg tatagctaaa gtagccctcc agcagagcct
    10261 ggaacagctg cggcaggcgc tgagtgtaac cattggcatt aaccataagg ccgttgttag
    10321 cattggtgga aaaacttatg ccaccaaccg acgcctggtt gcttaactga tcaagcgcca
    10381 gccctgcgag ataatcattg agcgcaaaca tcacctgatt gcgggcgctg tccatggctt
    10441 tcggattacg caaaatcagg ctgacatcag ctttgggctc actggcaaaa taacggcttg
    10501 gcgcatacac cacgcgcaga ttcgactcat caacaatcaa ctctggatgg tcgtatttct
    10561 tctctgactt aatcagcgag aaatcgtcag gaatataggg gttaagctcc ggtaaggaga
    10621 gcgcaatatt ggcggctttt ttctgccagt ccgcgaaagt ttgttcgctg attttatcga
    10681 cttgatacgg cgcatcgaca aaataagccg ttttattgtg cggctctttc gggctgatat
    10741 accagatacg cgcattctgc ggcgtcatca tcgccagacg ttcttttact gctttagcat
    10801 cgtaccgatc ggcaatattg actgcatcca gtgtatgctc aacaggaacg cgaatcatgg
    10861 tatcagccag ccattcgacg taatccatat cacgggtgat tgacggataa cggaagtcga
    10921 tatccagcac attcgccagt tcatcgaagt attgtttatc gatccctttt tcacgtaaca
    10981 gattgagata gctaaaaatg gccgccacaa cctgatcgcg attcgccaga cctttatcgg
    11041 ttaaagacgc agagatcgct aatacgccgc tgttgccgtt gacgatagga tcggaattgg
    11101 cgctaatacc ctcaactaat ccctgctttt gcagccagtc agaaagtgta ccagggctgc
    11161 gattaccaat cagataggta atcaattcat ccgttttact acggaacttc gctgaattgt
    11221 tatcgatgcg aaactcaacg cgcagaactt tacgcggcaa cgccgggacg taatgaatga
    11281 taatgccctc ttgcgcgtcg gtgactaccg gcacggtgat ttccggtttt ttgctctctt
    11341 tgttcggcac gcgaccaaag gtgtccgccg ccattttcgc caactccggc aacggtttat
    11401 tgctgtaaat aaccgccttc atcagattgg cggaatagta cttctcgtgg aaatctttca
    11461 gcgcctgctg taccggatta cctggtttgt cgcttaaagt ttcgaggtta ccaccagaaa
    11521 actttgaacc ggggtgtgcc gggttaatgg tttctgcgct gacctgtgcc atgcgcatcc
    11581 cgtcacgcgt acgcgccatg gttaattcgg cattcacggc attacgttca cgttcggcgt
    11641 atttcttgtc gagcaagggt tctgcaatag cgtccgccag gcggtctacc gcaccgggca
    11701 aggcgtcgtt ctcaacttcc agatagaaag ccgtgcgata cggtgccgtg ctggcattgt
    11761 gactgccgcc gtgcattttg agatattcgg ccagactgtc agcctgcgga tactttttcg
    11821 accccatcag actcatatgt tcaaggtaat gtgccagccc ctggtacgcc tcaggatctt
    11881 ccagcgaccc aacgggcacc accagcgccg agagcgattt aactgcctgc ggatcagaaa
    11941 ccagtaagac caccataccg ttatccagac gtatagcctg atactggcgg ttatctttat
    12001 cacttttacg gatggtttcc tgaatcggct gccatcccgt ttctgcctga cttaagggtg
    12061 cccagagggc aacaaacaac aataatgctt tgaaccagat gctgcggggc attcacggac
    12121 ctcataatca actttatttt ctggccagat tcaacacgtt aacgcaaatc atctgccaga
    12181 atttaatctt gtgctgaacg agtcagccta tgtctatata accatcagtc cgtgactggt
    12241 gcgcatcata aagtaagcgg atagattgtg caatttttat acaatactca tgactgatta
    12301 aagcgaaaca gcggtaacag gaaacgttgc gactgttcaa cgatagcctc cattgtctct
    12361 ggtgttaatt gccgccagag ccgttgatac cagatatcat caccttcgcc acgcaccatc
    12421 atgttgcctt cgtaagcctg aaggaatttc gtacgggctt tttgcaacgt ggaatcgtca
    12481 tccagcatgg catcgttttg cgcgtcataa caggttttta gccacgcgcc gccactttca
    12541 ggtaacacca gcaatggcgc ggacattcct tcacgatacc cttcaatcag ctgtgagagg
    12601 taatgtaaag cctgttcggc tgcaagcggc ggaaaacgcc actcgccgtc tttgcgtaga
    12661 aaaaggcgac tttcaccatt accaccgctg gcacagtaga caaggtgttc cagccaaagt
    12721 tgcattccct gcgccacact taataaagag ggacgccagc gcaacaggcc atccggctgc
    12781 acctgcggca accagccagt tatctgcaca ccgttgcagg cgagatcgat ttccatgctc
    12841 tgccctggct ggcgacaggc aatgactctg tcggcaagtt gctgcatctc ctgacactgc
    12901 gtttcccaga aaatttcacc aaaggcacca tacggcaaat cacctgccgc ccggaatcgg
    12961 cggaacaagc gttcggcatc atcctgctca accagtacat ttaataactg ctggttgatt
    13021 tgataacgac taagcccttc aagaataaaa ggttcggtgt caggaatttc gctgtcttcg
    13081 gtacggaagt tcacctgcaa acgcatctgg aaaaacgccc gcaccggatg tgcccagaat
    13141 cgttgtagcg tctccagcgg cacagtttcc ggcaaggtaa acggcagcgg ctgaacaaat
    13201 tcagaatgtg ctttaccagc ctggctggcc gcaggtagcc actcgcgcgc atagctttgt
    13261 aagttaccgg gctggtagtt ttgcgggtca aacggcatcc gggtatggtg gcaagtaaga
    13321 tgcgctttta cccttgcctc gctttcatca cagttaagcg cttcatcgcc cggtagataa
    13381 tggctttgcc cgatgtagtc gatcagttcc tgcaccagta ccgacgggaa acgttcactg
    13441 ttatcctgaa tggaacgccc gatatagctg atatagagtt tttgctgcgc ggaaattaac
    13501 gcttccagga acagatagcg gtcgtcatcg cgacggctac ggtcgccacg cttcggtttc
    13561 tggctcatca gatcaaagcc caatggcgca agctgacgtg gataaacgcc gtcgttcatt
    13621 cccagcaggc aaaccacttt gaacggaatt gaacgcattg gcatcagagt acaaatgtta
    13681 accggtccgg cgagaaaacg ctggctgata cgttcttgat ccaggcgctg tgccagttca
    13741 tcacgcaata gtgacagcgg caccgcgtcg ccatactgcg cacctaaacc ttcggcgata
    13801 atcgcctgcc attgttgttc gatcagcgtc atcgctgctt cggtttccgc atccggcagg
    13861 aagaaggcgt tgagcatatc gcgacaaacc ggcaaccact cttccagcgg acgctcctgc
    13921 gccagcccgc gacgccagat gtttagctgc attagcagtg aagacagatg ccccaccagt
    13981 tctgcaatta agccgctcga ttcatcataa ggtagaaccg attgccactc gccctgcgcg
    14041 ctctccatcg cgtagcccag caacatgcgc gtcaggccaa accgccaggt gtgttgaccg
    14101 gtagcgggaa gttccagctc gcgaacgttg tcgtcatcta tcccccaacg aatgccggat
    14161 tcgttgaccc actgacgtaa ataacgcagc ccttcttcgg tgatgtcaaa ccgcgctgcc
    14221 agcaccggca catccagtaa tgccagcacg tcttccgaca caaagcggct gtctggcagc
    14281 gataacaggc tgataaacgc ttcaagtaca ggatgcgact gccgcgcccg acggtcggaa
    14341 atggcgtaag gcaggtaacg atccgcaggt gcactaccaa acacagcctg aataaacgga
    14401 ctgtagctgt cgatatcagc caccatcacg atgatgtcgc gcggagtaag tgtcgggtct
    14461 tcctccagca tcgccagcag gcgatcgtgt aaaacttcaa cttcacgctg cgggctatgg
    14521 caaacgtgga aggtgatact gctatccagt ggatcaagcg ggcgtttgtt atcgctacgg
    14581 gaaaactctt cgatgttcac accagcaacg gcgcggtttt ccagttccag aatgtcagac
    14641 tgaatattat gcagcaggtt atctggcgtc acatcgacaa aagcgtccag ctcctggctg
    14701 ctctccaggt cagaaaggag ataaatgtag tcgcgcccaa gcttgcccca tgaagccagc
    14761 agcgggttgc cgacatcctg ttcaccatcg ctgttaaaga gctgcccggc attttcgctg
    14821 tcgcgaaata gcggtaattc gcgatcttca aaactgtgtc ggcgctggcg agtcagtagt
    14881 ttcgccagat aagctggatc tttaatgtcg ccccagtaat aacggcaggg gttggtaaac
    14941 aggagatgga tttcaatatg tttacccagc gcctgtagcg cctggagata aacaggcggt
    15001 aacgcggaaa taccgcatat aaagacgcgc gaaggtaacc ccggcgggca ggtcgtcgcg
    15061 gactccagcg tttcgataaa gcgctgatag agattggcgc ggtgccagcg cggttgccca
    15121 agttcgtcgg tatattccac caacgccttc cacaacggcg cttgccaggc ctgtgcttct
    15181 cccaaccctt ctaccagatg tcctgtttcc cactgtgcca gccagtccgg acgatagacc
    15241 agatactggt caaacaggtc cgccgctttt gaagaaagct ggaacagttt tcgcttgtca
    15301 ctatcgtcag tcagataatg ccgcaacagg gtaaagtctt cgcgctccaa cagttgcggc
    15361 agcagagtca tcagtttcca gctcatgctc tgtttgttaa aggcgctctc tttggggatc
    15421 tccggtaaca cccggacgaa catatcccag ataaagctcg ctggcagcgg aaaatcaatg
    15481 tttgccgcaa taccaaactt ttgcgacagg gtcatttgca gccactgtgc cataccggta
    15541 ctttgcacca gaatcatctc tggttcgaaa ggatcgtcca gccgttcgcg ttcgacaata
    15601 aactccatca acgcttccag cacgtccaga cgattggaat ggtagaccct taacatagcg
    15661 gctcctgact actgacgatt cgggcaatgc aggcgggtca tctcgccttc tctgcccccg
    15721 ggtgaaacta gcgtaacgct gatgctgaca catcccgcct gcgatgtctg cattcggttg
    15781 acctgccagt tagcaggtgg cgaaatcgcg cgcagttgcg tttgctgcca gccatgccgc
    15841 cagagctgtt ggtactggtt tctgctggca agactgttca ttaatgttcg ctggaaaccc
    15901 gataacgcag tgacaatcat caccatcaac accatcgcca acattacctc cggcagacta
    15961 aagccttgtt gattcctcag ggaagctgac ataacgccct ctctttcagc ggacaaaaat
    16021 cgctccagcc gcgtggcgaa aaaacaatgt ttccatcgat gacttcgcct gttcgccaca
    16081 acgaaacgcc ttcataacca gcaatcaata aggcttcatt atctgccagt aaacgcagac
    16141 aaacccgggc accggtggca gcatacagta agcactgaac tgcgggctgc gtctgccagg
    16201 agtgcatttt tccccactcc agcgccgact gaacgatggc ctggcggcgc aatgactgac
    16261 tttccatgct cacgcgagaa gcaaaactgc gatcctgctg actcattcct tgcaatagca
    16321 agctacccaa aatcagcagc atcaggacca gagccagtga cgaaacaccc ttttcgcggt
    16381 tcacaggttg gatcctgtca cgctatagct ggcatccacc acggtttgcg gatcagcctt
    16441 gctggcagcg tgcatattaa ccgtcaacac cggcgagaag ccgctgacat cctgacgtac
    16501 gacctgaaaa gtgtcgataa tgatggcatc cggattagtg actttatccc agcccttacc
    16561 ttcacaggat gtcgcaccgc gtagcgtttc cagcacatgc tccttcagac gaaatccaat
    16621 ctggtcggac tcttttaccg gttcgcgatc ccagatacca ttactgttcg catcccactg
    16681 cacaatgaca cagtcaccct gtccgacaat ttccagccct tcgcccgtac agctgccatg
    16741 gcaataaccc gccctctgga gatgcttcgc gacggtaaat acccgcagcc agatttcatc
    16801 ttccagcgcc agcttacggg tgttcgttaa actttcacgc tgtaacgcag gcagaaagcg
    16861 tgcagccccc agcaacaata cgctactgat cgccatagca atcaacactt ccagcagaga
    16921 aaaaccttgc tcttttacag gcatccttct gtttctcctt gctgacaaag ccggagtctt
    16981 ccccacggcg aaaccaccag ccaccactcg cccgttgagt ttttgaagcg aatatgcccg
    17041 gcccatgcgg tattgcgcag gccaaagaaa gcaagcgaag gtgtcaggtc gctcatttcg
    17101 acttcgggcc agcgcggcac aaagaccaat ggtgaactgc catgacaggt attcgcccca
    17161 gcaacggaac tcacaaggca ccataacgtc ccctccctga taagactgat actgtggtcg
    17221 cggttatgcc agttggcatc ttcacgtaaa tagagcaaat agtcccgcgc ctggctggcg
    17281 gtttgccaaa gccgttgcga ctgctgccag tattgccagc cataaagccc acttgcgctt
    17341 agcatgacca gaatcagcat cgcgaccagc gtttcaatca gcgtataacc acgttgtgtt
    17401 ttcatgccgg cagtatggag cgaggagaaa aaaagacgag ggccagtttc tatttcttcg
    17461 gcgcatcttc cggactattt acgccgttgc aggacgttgc aaaatttcgg gaaggtgtct
    17521 cgaagaattt aacggagggc aaaaaaaacc gacgcacact ggcgtcggct ctggcaggat
    17581 gtttcgtaat tagatagcca ccggcgcttt aatgcccgga tgtggatcgt atccttcaat
    17641 ctcaaagtct tcgaaacggt agtcgaagat ggattcgggt ttacgtttga taatcaactt
    17701 cggcagcgga cgcggttcgc ggcttaattg cagatgagtt tgatccatat ggttgctgta
    17761 gagatgcgtg tcgccaccgg tccagacaaa atcacccact tccagatcgc actgctgcgc
    17821 catcatatgc accagtaacg cgtagctgga aatgttgaac ggcaggccga ggaagacgtc
    17881 acaggagcgc tgataaagct ggcaagagag tttgccgtct gccacataga actggaagaa
    17941 tgcatggcac ggtgccagcg ccattttatc cagttcgcct acgttccacg ctgaaacaat
    18001 aatgcgacgg gaatccgggt cgtttttcag ctggttcagt acagtagtga tctggtcaat
    18061 atgacgacca tccggcgttg gccaggcgcg ccactgttta ccatacactg gcccgaggtc
    18121 gccgttttca tcggcccatt cgtcccagat ggtgacattg ttttcgtgta gataagcaat
    18181 gttagtgtcg ccctgcagga accacagcag ttcgtggatg atggaacgca ggtggcaacg
    18241 tttagttgtc accagcggga atccatcctg caggttaaaa cgcatctgat gaccaaaaat
    18301 ggaaagcgtt ccggttccgg tacggtcgtt tttctgtgtg ccttcgtcga gcactttttg
    18361 catcagttct aaatactgtt tcatggttcc tcaggaaacg tgttgctgtg ggctgcgacg
    18421 atatgcccag accatcatga tcacacccgc gacaatcatc gggatggaaa gaatttgccc
    18481 catgctgatg tactgcaccc aggcaccagt aaactgcgcg tcgggctggc ggaaaaactc
    18541 aacaatgatg cgaaacgcgc cgtaaccaat caggaacaag cctgagacag ctcccattgg
    18601 gcgcggttta cgaatataca ggttgaggat aataaacagc accacacctt ccagcagcag
    18661 ctcgtaaagc tgtgatgggt ggcgcggcag cacaccgtaa gtgtcgaaaa tggattgcca
    18721 ctgcgggttg gtttgcagca gcaaaatatc ttctgtacgg gagccaggga acagcatggc
    18781 aaacgggaag ttcgggtcaa cgcggcccca caattcaccg ttaataaagt tgcccaggcg
    18841 cccagcacca agaccaaacg gaatgagtgg ggcaataaaa tcagagacct ggaagaagga
    18901 acgtttagta cggcgggcga agataatcat caccacgata acgccaatca ggcctccgtg
    18961 gaaggacatg ccgccgtccc agacacggaa aagatacagc ggatcggcca taaactgcgg
    19021 gaaattgtag aacagaacat aaccaatacg tccaccgagg aagacgccga ggaagcccgc
    19081 atagagtaag ttttcaactt catttttggt ccagccactg cccggacgat tcgcccgtcg
    19141 tgttgccagc cacattgcaa aaatgaaacc caccagatac atcaggccgt accagtgaag
    19201 cgccacgggt cctattgaga aaatgaccgg atcaaactcc ggaaaatgca gatagctact
    19261 ggtcatctgt caccacaagt tcttgttatt tcgctgaaag agaacagcga ttgaaatgcg
    19321 cgccgcaggt ttcaggcgct ccaaaggtgc gaataatagc acaaggggac ctggctggtt
    19381 gccggatacc gttaaaagat atgtatatga tccgcgctat aaccccccgc gaatcagccc
    19441 gcccatgccg cgacgctcca taaaggcagc aacctgatgg cgaacttcgg tcgccagttg
    19501 cgcttccaga ctacgctgcg caagattttc tgcttcagca aaatcaatgc gccgcagcag
    19561 gtattttacg cgcgctacag aacgtccgtt catagacaga tggcgatacc caagcccaat
    19621 gaggattgcc acgcacatgg gatcgcccgc catttcaccg cacaaacgga gatcgattcc
    19681 atgtatttcc gcttcccggg cgatcatcgc cagagctcgt aacattgcag gatgaagact
    19741 gtcataaatg ttcgccaccc gggtattgtt gcgatcaacg gccaggatgt attgagtcag
    19801 atcgttggtg ccaacagaga tgaaatcgac ccgctttgcc agatgcggca gcataaatac
    19861 cattgacggc acttccagca tgatgccgat acgtggtttg ggaatttcgt aaccgatcat
    19921 ctcctcgact tcacgtccgg cacgttcaat caggcggcgt gcttcgtcaa cttcatcgag
    19981 gcttgtgacc atcggcaaca gaatattcag gttgcccgta gcggcattag cacgcagcat
    20041 cgcccgcacc tggatcaaga agatctccgg ctgatcgagc gtaatgcgaa tcccacgcca
    20101 acccaggcat ggattctctt cgctaatcgg catgtaaggc agctgcttat ctgctccgac
    20161 atccagcgta cgcaaggtga cgggtttatc attaaacatt tgcagcatcc cctgatactg
    20221 cgccacctgt tcttcttccg acggaaaacc actttgcagc atgaatggga tttcagtgcg
    20281 ataaagtcct atgccatcaa tacggctgcc cagtttttct tcatgttccg ggcttaaacc
    20341 ggcattgagc atgactttaa tgcgttcgcc gctttttaac tgggcgggta aattgacgtc
    20401 atcttccgcc agacggctaa gctcgatctc ttcactaatt agccgctgat attcttgcag
    20461 cagtaccggt tccggatcga ccagcaattc accgcgataa ccatcgacga tcagcgtccg
    20521 acgatgcagt accgaaggct gaatatccgc gcccatcacg gtaggtatcc ccagcgcacg
    20581 taccatgatc gcagcatggg agttggcagc gccatcgcgc acgacaacac cgactaagcg
    20641 atcctggggc agctcagcaa gcgttgtcgc tgacagttca tctgccacca gaatgaaacg
    20701 ttccggccag gcgttcggcc cttgattagc gtcatcaaga tgaaacagca atcgctgacc
    20761 cagcgcacgt aaatcgccag cccgctcttt gagatagtta tcgcttagcg cggcaaactg
    20821 ttcggcaaat ttttcaatga ccgtttttac cgcccactct gccaccgagc ctttatcaac
    20881 ctcggcaaac aattcgcgac gcagccgggt atccgaaagc aggtgtgagt aaagatcgaa
    20941 aatagccgcc gtttcttttt gtgcaccggc agcaaagcgt ttgctatagc ggcgaaactc
    21001 gttagccgcc tcttccaacg ccccggtcag tcgttcgcgt tccagagccg gatccagcgt
    21061 tgatgcctga tacacctgtt ccattaaagg taacgtggca tcctgccagc cttcggcaat
    21121 cgccacacca ggtgctgccg gtaatgcacg gattcgcgtc tggcgatatt gcccaaacaa
    21181 ggcagtcaac tgcgactgag aaagaatagc tgccatctgg gtggcaagcg tcaccaggaa
    21241 ggattcttca ctttcgtcat actggcgcaa ctctcgttgc tgtaccacca gtacaccaag
    21301 caactggcga cgttgaataa ttggtacgcc taaaaacgcg cggaaacgtt cttcttttac
    21361 ggaggggatg tatttgaagc tggggtgctt ttgcgcatct gcaaggttta tcggttccgc
    21421 cagcctgcca accaggccga cgatcccttc atcaaacgcg agcgttacag tgcgaccgcg
    21481 tggttttttc agtccccggg tcgccatcag gtagtaacaa cgtcgatcat gatcggccag
    21541 gtagaccgaa cagacctcgg tatccatcgc aagacagatg tcggtaacca gaatatttaa
    21601 cgcctcattc aggcgtggtg cgctggctac cttttcgact atttcgcgca ggcgagtgag
    21661 cataattggc gtgacttaac ctcttttacg tcgataagca gatgcgtttt gtggttttgg
    21721 cgtattttcc tgcagtgaca tcaccacact cgcgaactct ttcattaccc tacggtagac
    21781 atcacgttta aatgacacca cctgtctgac tggataccag taacttaccc atcgccagcc
    21841 gtcaaactct ggtgtactgc tggtttgcat attgatttct gcatcgccgc tcaccagctg
    21901 caagagaaac catttttgtt tttggccgat acaaaccggc ttcgtgtccc aacgcaccaa
    21961 acgtttcggt aatttgtagc gcaaccagtt acgcgttgaa gcaaggattc ggacgtcttt
    22021 gcggcttaat cctacttctt caaacaattc acggtacatc gcctgctctg cggattctcc
    22081 ggggttgatt ccgccttgcg gaaattgcca ggagtgctga ccaaatcgcc gggcccacat
    22141 tacctgcccc tggcgattac aaatcacgat acctacgttt gggcggtagc catcgtcatc
    22201 aatcaccgga ctacctcaaa ataaagcttt atatacgaat gattgtttca tactccagga
    22261 agacggtaaa ccactctctg cagggcatta cacactaata acaattgaat aactcacagt
    22321 tatgtgcaga gttataaaca gaggaagggg tggatagccg tttttattca ccttttctgt
    22381 ggatagagtt gtgaagaact acggaattac tacgggaaaa cccggagcat tctgaataag
    22441 acagatatag agattttaaa atattcatta ttagtcaatg cattacatgt gatttgagta
    22501 ataaaaaaga gtaattcgtg acccaggtca cacctctcat ttacgtgttg gcgaaagatc
    22561 aaccaatgcc gtatttatcc acagaatgtg ccactaagtt aagcactgaa ccactaaaaa
    22621 ctggagtttc gtcgcacgtc aaggctgtaa atggaaacag tagtggaggt ttttcacagt
    22681 tatcccagct ttctgtggat aacatggtgt aagatcctgt ttattttcag tgaccagatt
    22741 tggaaaaccc gttgcagtgt tgcgcaactc gtttaccggc aacctaaaaa gcaatataaa
    22801 tcagagaatt gaacaacgca tgtggaaaaa gttacactgc gaatattcag cacataattg
    22861 ctgtttgttt tttaaccaag gtatcatgac atgtcccaac ctcgcccact gctctctcct
    22921 cccgaaactg aagaacaatt gttagcgcaa gcacaacaac tttctggtta tacattggga
    22981 gaactggcgg cacttgccgg gctggttacg ccggagaatt taaaacgcga taagggctgg
    23041 attggcgtgt tactggagat ctggctaggt gccagcgcag ggagtaaacc tgagcaagat
    23101 tttgctgctc tgggcgtgga acttaaaact atccctgtgg atagtcttgg tcgtccactg
    23161 gaaacaacat tcgtttgtgt tgccccgtta acgggcaata gcggggtgac ctgggaaacc
    23221 agccacgtgc gccacaagct caaacgcgtg ctgtggatac cggttgaagg cgagcgcagc
    23281 atcccgctgg caaagcgtcg cgtaggatca ccgttgctgt ggagcccgaa tgaagaggaa
    23341 gaccggcagc tacgcgaaga ctgggaagaa ttaatggata tgattgttct cggtcagatt
    23401 gagcggatca ccgctcgtca cggggaatat ttacaaatac gaccgaaagc agcgaatgcg
    23461 aaagcgctga ccgaagctat tggtgcccgg ggcgaacgga ttctgacgct gccgcgcgga
    23521 ttttatttga agaagaattt caccagtgcg ctactggccc gtcattttct gatccagtag
    23581 ccatcgcttt gacctgctgc tttccgggca tataattacc gcttcatttt tttggcaggg
    23641 ctttttagat gttatttgca tggataaccg atcctaacgc ctggcttgcg ctcggtacac
    23701 tgacgctgct ggagatcgtt cttgggatcg acaatattat tttcctttct ctggtggtgg
    23761 caaagcttcc cacagcacaa cgtgctcatg cgcgccgtct ggggttggcg ggagccatgg
    23821 ttatgcgtct ggcgctgctg gcatccatcg cctgggttac gcgcctgacg aatccgcttt
    23881 ttacaatatt cagtcaggaa atttccgccc gtgatttgat cctgcttctg ggtggcttgt
    23941 tccttatctg gaaagccagc aaggaaatcc acgaatccat cgaaggtgaa gaagaagggc
    24001 tgaaaacacg cgtttcatca ttcctcggcg cgatcgtgca gattatgctg ctggatatta
    24061 tcttcagcct cgactcggtg attaccgctg tgggtctgtc agatcacctg tttattatga
    24121 tggctgccgt ggtgattgcc gtaggcgtga tgatgttcgc cgcgcgctcg attggtgatt
    24181 ttgtcgagcg ccatccttcg gtaaaaatgc tggcgctctc tttcctgatt ctggtgggct
    24241 ttaccctgat tctggaaagt ttcgacatcc acgtaccgaa aggttacatc tacttcgcga
    24301 tgttcttctc tattgcggtt gaaagcctca acctgattcg caacaaaaag aatccgctct
    24361 gatactccgt acgctctcct gcgggagggc gtactcactc ctgttaccct cacagtttaa
    24421 gattttcctg ctttcaagat atataacgtc ggtttataaa cagactatta tcataggtgg
    24481 gccaaatgag gataaccgga tgaaaaaatg ggcagtaata atttctgcag tcggactggc
    24541 gtttgctgtt tccgggtgtt ccagtgatta cgtcatggcg accaaagatg gccgtatgat
    24601 tttgaccgat ggaaaacctg aaattgatga tgataccggg ctggtgagtt atcacgatca
    24661 gcaaggtaac gcgatgcaaa ttaaccgtga tgatgtttcg caaattattg aacgttaaca
    24721 aataaggtca gcatccggct ggccttaaga tttttctctt tcccttttcc cttccctctg
    24781 ccatttttat attccttatg tcgtgattat aaaaaggaaa cggctatgca atatcaccgt
    24841 ataccccaca gttcgctgga agtcagcacg ctggggcttg gcacgatgac gtttggtgaa
    24901 cagaacagcg aagccgacgc ccacgcacaa ctcgactatg ccgtcgctca gggcattaac
    24961 cttatcgacg ttgccgaaat gtacccagta cctccgcgcc ccgaaactca agggctaacc
    25021 gaaacctacg tcggcaactg gctggcgaaa catggcagcc gcgaaaagtt aattatcgcc
    25081 tccaaagtga gcggaccgtc gcgcaataat gacaaaggca tccgcccgga tcaggcgctg
    25141 gatcggaaga atatccgcga agcgctgcat gacagcctca agcgtctgca gactgattac
    25201 ctcgatctct atcaggtgca ctggccgcag cgcccaacca actgcttcgg caaactcggt
    25261 tatagctgga cagattctgc gcctgcggtt tcgctgctgg atacgctgga cgcactggca
    25321 gagtaccaac gcgcgggaaa aattcgttat atcggcgtgt cgaacgaaac tgcatttggc
    25381 gtaatgcgct acctgcatct ggcggacaaa cacgatctgc cgcgtattgt caccattcag
    25441 aacccctaca gcctgttaaa ccgcagtttt gaagtaggtc tggcagaagt cagccagtat
    25501 gaaggggtcg aactgctggc ctattcgtgc ctgggtttcg gcacactgac cgggaaatat
    25561 ctcaatggtg caaaacccgc tggcgcacgt aatacgctct ttagtcggtt cacccgctat
    25621 agcggtgagc aaacgcaaaa agccgtcgcg gcgtatgttg atatcgccag acgtcatggc
    25681 ctggaccctg ctcagatggc gctcgcattt gtacgccgtc aaccgtttgt tgccagcact
    25741 ctgctgggcg caaccacgat ggagcagctg aaaactaacg tcgaaagttt gcatctggag
    25801 ttgagcgaag acgtattagc tgaaattgaa gcggtgcatc aggtttatac gtatcctgca
    25861 ccataagatt aacgccggtt aaaaccggcg ttaaatatta atgacggcgc tgccagatcc
    25921 acagcgccgt tattgccagc gcaaacagcg caccgaagcc aatgccaatg ggcacgaccg
    25981 ggatgcctac cattaccgcc agcgagtaaa tgcccagcat caacaacatg gcgctgtttt
    26041 cgccaaggtt ttgtactgca atcgcattcc ccgccccgac gcttttttta ccccgctcct
    26101 gtagcaacgc attgagcgga acgacaaaaa agccccccag cacgccaatc agcatcaaca
    26161 aagcataggc tggcagcagc tcgtgttgca gggagaaaat cagtaccacc acgccaatca
    26221 aaatcccggc tggcatacag cgtgacacgg tttccagcgt aactaacttc gctgccgcac
    26281 ctgcaccaac cacgatacca atcgctacca tcgcgttgag ataggtcggc gtagcgttat
    26341 cggtaatacc cagcgccacc ggtacccaca gcaccaacag gaaacgcagc gtgacacccg
    26401 ctccccagaa taaactggtg cccaccagcg aaaaacgcgt ttcaccattg cgccatagcg
    26461 aggtgcaggc attcaggaaa ctgcgggtca tgctgatgag attccaggac tgccccggac
    26521 gcgccgccgc cagtttggga atgtagatat tggcaacgac cgcgccaccg taggccagtg
    26581 cgcatacggc cagggcaacc agaatatgcc agtcagccag aacaccacca gctacggaac
    26641 cgagcaaaat cgccgctatg gtagacgctt ccattaaacc gttagctttt actaacttac
    26701 tacccgtggt taattcgccg agaataccgt atttcgccgg tgaataggct gcagcaccaa
    26761 cacccaccaa cgtatagccg agaaacggat tgataccaaa gcagatactg gctgcgccca
    26821 gtagcttcag gccgttggca aacatcatca cccggccttt ggcgaagcta tccgccacct
    26881 gcccgacaaa cggcgcaaaa agaatgtaag cacctacaaa caccatttgc aggatgggct
    26941 ggctccactc cggatagaac tgcgctttca gtaacgccag agtggcaaac agtagcgcat
    27001 tatcgccaaa cgcagagaga aactgcgcca cgataaccgc tttcatcccc ttcgaccaca
    27061 acgaagtgtt agtgtgcact gactcactca tcgtgttgtt ccgcttcgtc tacccagctt
    27121 ttcaaagtga caaagtcagg tttgccgctg ccaagtaatg gcatctgttt cagatagcga
    27181 atatcgcgcg gtacagcaag ctccggcacg ccgtgctcgc gggcatactg ttgcaactta
    27241 tcgcgcgtca gttcgttatc tgtggtgaaa agcaccagtg cctcgccttt gctggcatcg
    27301 ctcttaatcg cagtggcatg gactttatct ggcgaaacac caagtgccag ttgttccacc
    27361 atttccagcg acaccatttc gcctgcaatt ttggcaaagc gttttgcgcg gccctgaatc
    27421 tgcacaaagc cctgctcatc aaaacgcacg atatcgccag tgtcatacca gccgcgctcc
    27481 atttcgccgc gaacattctc ggcggtgggc acttccagta caccaggctt ctccacccgc
    27541 agatagccgt tcattatgtt cggccctttc agttgcaggc gtccgccctc ttcgatacct
    27601 ggaactgaca acaggcgcgc atccattcca ggtagaatac gccctaccgt accgggtttc
    27661 gccgccatcg gtacgttgat agaaacgaca ggcgcgcatt cggtcacacc gtagccttca
    27721 aggatgcgca ggccaaattt atcctgccaa agctgtttag tactttcttg tagtttttct
    27781 gcgcctgcca ccacatagcg tagacgatag aagtcatacg ggttggcaaa gcgcgcgtag
    27841 tgaccgagga aagtcgaggt accgaacaac acggtgcaac tgcggtcata caccaactcc
    27901 ggcacaatgc ggtaatgcag cgggcttgga taaaggaaca cttctgcacc tgtgagcagt
    27961 ggcgtaaaca ggcctaccgt cagcccaaag gagtgaaaca gcggtaacgc cgacataaag
    28021 cgatcgttgg tggtgaagtc ggcaatcgtt ttaatctgct cgacattcgc cagaatgctt
    28081 ttatggctat ggacgacgcc tttcggatgg ccttcagaac cggaggtaaa aaggatcagc
    28141 gcctcttctt ccggctgctg tttaacctgt gccagacgcg gcatcagcaa atgagcgaag
    28201 atccatactt tgtcggcagt ggtgacatct gcttttaaat cttccagata gacccagcgc
    28261 acctgagtaa gttgctccgg cagatgccag agtttgcctt tatcgagaaa ctgacgggaa
    28321 gtgaaaatgg ttttgatttc agccgccgta atagcactgg tcaacccttt taccccggcg
    28381 gtgtagttca tcattgcggg gatgcggcga cgggcgatgg ccccaaaaat cactgcagca
    28441 ctgatgcctg cattgggcag cattaagccg atgcgttcgc cttcaacact gtatttttca
    28501 aggatgcgtc caacaaacag cgttttcgta agcaatttgc gataggagtc cggggtaaag
    28561 ttaacgtctt cgacacattt cttcccggct ccgaagcggt acattgcgct cagtaaagat
    28621 tcgtacagcg tttcacgcgg gcgcaccgcc atacgcgctt ccatcattat ttgatgcagc
    28681 atttcgccag cgattttgcg acggtcacgg gcacgcggcg catccggcat ctccacctgc
    28741 gttggtggca aaatatgcag agtaatttgc gggaacaagc gacgtttaac cagacctttc
    28801 aggcggctga agtgcgtaag ttccgcccct tcaatacgca caggaataac cgttgcacca
    28861 gacttcgccg cgacaaatcc cgcgccatcg tagattttca tcagcgagcc agtggtggtg
    28921 atgcgtcctt cagggaaaat caccactggt cgtccctgtt ccaccagacg taccagatgt
    28981 ttaatcgcca taggttgcgt cgggtcgaga ggaacaaagt cgataaatga tttcagccaa
    29041 cgcatatacc actgttggct tattgaggtg taaacggcaa ataccggacg tacgggtaaa
    29101 aacaatgcca gcagtatgcc atcaataaaa gagacgtgat taggcgtaat tagaacgcgc
    29161 tcgcccttca gtgccttggt gtcacccgta acgcgaacgc gatacaaaac acggcacaaa
    29221 tttcgaaaaa agctaaaaag cattccaact ccctttgctc tgattcagta aaagcgaatg
    29281 gagggagatt acacgagata aagaacgcga gcgacagtaa attaggtgcg aaaaaaaacc
    29341 tgcgcatccg cgcaggttgg tgcaagagac tgggtacgaa gagcgtaccg aataatctca
    29401 ccaatcaata cctctgggat cttgattgtg gtctgcacaa cgactcttcg ccagcgagaa
    29461 aacgcaaagg aatgaaggga aatgcaacga ggtgtgtaaa ttgtcggtta ctgttacaga
    29521 ttgatgaccg gcaaaaaaaa acctgcgcat ctgcgcaggc tggtgtaatt catgtgctca
    29581 acccgaagtt gacttcacct atcaatacct ctgggatcac cactttagca acctgaagcc
    29641 aaacgccacc agcggtcaat cgcaacagcg tttcgcaaag tgtaaccaaa ggtttgcaat
    29701 ctcttttttt gtcttactta tttgcgtttg gctcacattc ccacaatgaa aacacgccgc
    29761 cccttgaacc aacgggcgtt ttccgtaaca ctgaaagaat gtaagcgttt acccactaag
    29821 gtattttcat ggcgaccata aaggatgtag cccgactggc aggcgtttca gtcgccaccg
    29881 tttcccgcgt cattaataat tcacccaaag ccagcgaagc ttcccggctt gctgtgcata
    29941 gtgcaatgga gtctcttagc tatcacccga acgccaacgc ccgtgcgctg gcgcagcaga
    30001 ccactgaaac gatcggtctg gtcgttggtg atgtttccga tccgtttttc ggcgcaatgg
    30061 tgaaagcggt cgaacaggtg gcttatcaca ctggtaattt tttattgatt ggcaacggtt
    30121 accacaacga acaaaaagag cgtcaggcca ttgagcaact gatccgccat cgctgtgctg
    30181 cgctggttgt ccatgccaaa atgatcccgg atgccgattt agcctcatta atgaaacaaa
    30241 tgcccggtat ggtgctgatc aaccgtatcc tgcctggctt tgaaaaccgt tgtattgctc
    30301 tggacgatcg ttacggtgcc tggctggcaa cgcgtcattt aattcagcaa ggtcataccc
    30361 gcattggtta tctgtgctct aaccactcta tttctgacgc cgaagatcgt ctgcaagggt
    30421 attacgatgc ccttgctgaa agtggtattc cggccaatga ccggctggtg acatttggcg
    30481 aaccagacga aagcggcggc gaacaggcaa tgaccgagct tttgggacga ggcagaaatt
    30541 tcactgcggt agcctgttat aacgattcaa tggcggcggg cgcgatgggc gtgctcaatg
    30601 ataatggtat tgatgtaccg ggtgagattt cgttaattgg ctttgatgat gtgctggtgt
    30661 cacgctatgt gcgtccgcgc ctgaccaccg tgcgttaccc aatcgtgacg atggcgacgc
    30721 aggctgccga actggctttg gcgctggcgg ataatcgccc tctcccggaa atcactaatg
    30781 tctttagtcc gacgctggta cgtcgccatt cagtgtcaac tccgtcgctg gaggcaagtc
    30841 atcatgcaac cagcgactaa ccgcagttaa agcaattcca gcgccagtaa ttcttcaatg
    30901 gtctggcgac ggcgaatcaa ccgcgcctga ccattatcaa acagaacttc tggtaacagc
    30961 ggacggctgt tgtagttgga tgacattgat gcgccatatg cccctgtatc atgcagtacc
    31021 agataatcac ccgccttcac ttccggcaag gcgcgggttt cgacatttcc cccttcctgc
    31081 tgggtaaaga catcgcccga ttcacataac ggcccggcga cgacggtttc caccgttggc
    31141 gcgtgttcca gagaacgacc ctcagctgcc agggcactga tatggtggta actaccgtac
    31201 attgccgggc gcatcagatc gttgaacccg gcatcaacca gcacaaagtg gcggctaccc
    31261 atttgtttga cgctccgcac ttgagtaatt aatacgccag cctgcgctac cagaaagcga
    31321 cccggttcaa tttccagttt cacaggatgg cccaaatggc gggcgatttg ctcacgcgcg
    31381 gcattccaca gaccataata atgttcggta tcaaccgcct cttcaccctg ttgataagga
    31441 atagaaagcc caccgcccgc agaaatagcc tgtaaatcct gaccgaactc gaggacctga
    31501 cgcaccatag caccacatac ctgttccaga tgggcataat cgacgccaga accaatgtgc
    31561 atgtgaatgc cgaccagctg tagatgatga cgttgtatca cgtccagtgc ggcgggcaga
    31621 tcggtgtacc agataccgtg cttgctgttt tcgccaccgg tattggtttt ttggctatgc
    31681 ccgtgaccaa accccggatt aacacgcagc catacccgat gccctggaga aacctgaccc
    31741 agttggtcga gcatatcaac agaacccgca ttcaccggaa tttgcaattc actgacgcgt
    31801 tcaagcgtcg cctgatcgat aacatctgcc gtaaaaacca tatcatcggg gtgcgtttgc
    31861 ggattgtaac ccgccgccag tgcacgctct atttcgccta acgagacgga atccactttt
    31921 acgccctgct cacgcattaa gcgcaaaata tgaatattgg aacaggcttt ctgtgcaaag
    31981 cgcaccacat caaactgttt cagcgctgca atctgccgac gaataatttg cgcatcgtag
    32041 acccacaccg ggcagccaaa ttctgcgggc aaacgcagca gattttcggc ggtgagatcg
    32101 gtatcagtgc tgaacagtga atgtggcata acaaactcca gataagtgct tttttatgat
    32161 tacgccacat cataaaaaga ataaaaaata tcgatttatg tcgagtctat gcaaaaatga
    32221 tatggattac cggattgcga gagagcgcta atggccgcag ttaacttacg tcatattgaa
    32281 atttttcatg cggtaatgac cgccggaagc ctgactgagg cggcacacct gctacacacc
    32341 tcacagccaa ccgtcagccg cgaactggcg cgctttgaga aggtgatcgg gctgaaattg
    32401 tttgagcgca tacgtggacg attacatcct accgtgcaag gactgcgtct gtttgaagaa
    32461 gtgcaacgat cctggtacgg actggatcgc attgtcagtg ccgcagaaag tctgcgcgag
    32521 tttcgccagg gagaactgtc tattgcctgc ctgccggtct tttcgcaatc ttttttaccg
    32581 cggctcctgc aaccctttct ggcacgttat cccgatgtca gcttaaatat cgtgccccag
    32641 gaatcaccgc tacttgaaga gtggctctcg gcccagcgtc atgatttagg actcactgaa
    32701 acgctccata cgcctgcggg aacagaacgt accgaattac tctctttaga tgaagtgtgt
    32761 gtgttacctc cgggccatcc gctggcggta aaaaaggtat taacgccgga tgattttcac
    32821 agtgagaact acatcagcct ttcccgtact gacagctatc gccagttgct ggatcaattg
    32881 tttactgaga atcaggttaa acgacgcatg atcgtagaaa cccacagcgc cgcgtcagtc
    32941 tgcgcaatgg tacgggcggg ggtaggcgtt tcggtggtta acccgctcac cgcactggat
    33001 tatgcggcaa gcggtttagt ggtgcggcgg ttcagcattg cggttccatt caccgtcagc
    33061 ctgatccgcc ccctgcaccg cccgtcatca gcgctggtgc aggcgtttag tgagcattta
    33121 caagcgggat taccgaaact ggtcacttct cttgacgcta ttttgtcgtc agctacgaca
    33181 gcataaaagc gacagcatcc tcggcatgga tcgccgcggt atcaaacaca ggcagaacac
    33241 tgcgctcttc tggcaccagt aaaccaattt ctgtgcagcc aaaaatgacg ccctgtgcgc
    33301 cctgttctgc aaggcgagca atcacttgcg cataataagc gcgtgacgct tcggtaaatt
    33361 gccccagaca cagttcttca aaaataatct gattaatttt cgcccgttca tccgcttcag
    33421 gaataagaca gttgatggaa aattgttccg tcagccgccc gcgataaaaa tcctgttcca
    33481 tggtgtaacg tgtacccagc agcgccacac gagtcattcc tgccccggta attgcacgtc
    33541 cagtggcatc cgctatgtgt aagaaaggca gagagcaacg agactcaatg gcatccgcca
    33601 ctttatgcat cgtattggtg cataacacaa taccttctgc gcccgcccgc tgtaagccaa
    33661 gcgccgcctc agccagaata tccccggttt tgtcccactc accgcgacgc tggcactctt
    33721 ctatttcatg aaaatcgacg ctatgtagca gcacttgcgc agagtgaagc ccaccaagcc
    33781 gctgtttaat gccttcattt atcagacgat agtaaggaat agtggattcc cagctcattc
    33841 ctcccagcaa accaattgtt ttcattcctc tctcctgatg tgtgttaccg caacaaggct
    33901 gaataaggaa cgagacaaac gcctcaacgg ccaggtgccc aatctctatt aacgaaaaaa
    33961 gggccggatg tacagcacat ccggcccgtg aaatcagacg ccgatatttc tcaacttttc
    34021 gcctgccatc agtttgcgct cgatatgttc cagcgtgaca tttttggttt ccggaatgag
    34081 ccagaaagtg atgcccacaa acgcaatgtt cagcgcagtg tagagccaga acgtaccggc
    34141 agcgccaatg ctatcaagca gtgtcaggaa ggtcgcgccg ataatcatat tcgacaccca
    34201 gtttgtcgtc gttgagcagg tgatgccaaa atcgcggcat ttcagcggct gaatttcaga
    34261 gcacaagatc cacaccactg gcgcggcgct catcgcataa ccggcaatac acatcatcgt
    34321 catgccaaca gagagccagg acaagccact ggaagccgta ccgttatcaa actgcatcag
    34381 gcagtaaccc agcaccagag tgcctaacgc catcacgctg aaaccaattt tcagagccgg
    34441 cttacgccct gctttatcta ccgtaaagac cgcaatgaag gtagcgaaca taaaggtcag
    34501 ccccacgacc agagtcgcaa tcatctgttg ttctgtggtc gtaaagcccg ccattttgaa
    34561 gatacgtggc gcgtagtaca tgatgatgtt cataccggta aactgctgca tcgcctgcaa
    34621 caacataccg agaaatacag cacgacggac gttacggttg atcttaaaca atgcccaacc
    34681 gccctgtttt aacttcaggc tttcacgaat ttcgttgagt tcttctcgcg ctttttccga
    34741 cgtatcgcgc agcatacgca atacttcttc cgcctcaata tgacgcccct tttccgccag
    34801 ccagcgcggg ctatttggca ggaagaccac cagaataatc agcagaactg ctggtaaagc
    34861 aagaaccccc aacattgcgc gccagttacc gctataactg aacgctgtat cggataaaaa
    34921 cgccagcacg atgccgagag tgaccatcaa ctggtacatg ctgatcatct taccgcgaac
    34981 gttttcactt gccatttcag aaagatatag aggagcggtg taagacgcga tcccgacagc
    35041 aatgcccaac accacacgag cggcgattaa catctctacg ctggtcgcaa aagcggaccc
    35101 tatcgaaccg agtacaaaca ggatggcccc cgccatcagg ctgtatttac gccccaggcg
    35161 gaacgacagc caaccattaa acagcgcacc aattgctgcg ccgagcatca tgctactgac
    35221 cacccattcc tgcaaacgac tggtcagcac aaagtgatcg gtaatgaacg gcaacgctcc
    35281 ggcgattacg ccgatatcaa gaccaaataa caatcctgcg accgcagcag ctaccgaaac
    35341 aaacatattc atacgccgcg tatcccgcag aggacgtggc gttaaagcag attccgtatt
    35401 gatagtaacc attttttcct gccagcagag agtaagacat agtgaaaaaa tacgtgaaca
    35461 attcacacag gtgtcaggtc ggaaatagca taaatatgga ttaaattgct acgacatgtc
    35521 gttatgtgat ggatattcca attttcaaat taagttgaat tattgagatt attattaacc
    35581 acctaatttt aaagcagata aaattcataa agttcattaa ttgataattg atatggatta
    35641 tttcataacc atgatatgga ttatgatgat ctacagatat aaaaaaccct gccatgcggc
    35701 agggtcataa aagtaagagg aatgaattaa cgcgccagcc aaccgccatc cacagcaatg
    35761 gtataaccat ttacataatc tgaagcgctg gaggcaagaa acactaccgg ccccatcagg
    35821 tcactcggca gtccccaacg gccagctgga atgcggtcga gaatttccgc gctacgttgt
    35881 tcatctgccc gcagttgttg agtattgttg gtcgccatgt aacccggagc tatcgcatta
    35941 acattaatat tgtgtttagc ccattcgttc gccatcaatc gcgtcacgcc catcacaccg
    36001 ctttttgatg cggtataaga aggcacacgg atcccgccct ggaaggagag cattgacgcg
    36061 atattgataa tcttgccgcc attgccttgc gcgataaagt gtttcgccgc tgcctgagac
    36121 atgaagaata cgctcttgat attcaggttc atgacatcgt cccagtctgt ttcgctgaac
    36181 tcgagagcgt cttcgcggcg aatcaatccg gcgttattca ccaggatatc aatatgacca
    36241 aactcagcta ccgcgcgatc cagcagtgct ggaatgccat caatctttcg cagatcggcg
    36301 gtcaggctta aaaaacgacg ccccagcgcc gtgacctgct tgatggtttc agtcggttca
    36361 acgatgttaa tgccaacaat gtcacagccc gcttgcgcca gccccaacgc catcccctgg
    36421 cccagcccag tatcacaacc agtgacgacc gcaactttac cttcgagaga aaatgcactt
    36481 aaaatcataa caatacctta ttcgtttatg cccacaacta gcgcaaatct ttaacggcca
    36541 catggtccat atcatcaaaa acctggtttt caccgaccat gccccagata aaggtataag
    36601 ctttggttcc gacgccggaa tggatcgacc agctcgggga gatcaccgcc tgctcgttat
    36661 gcatcacaat gtgacgcgtt tcttgcggtt gccccatcat gtggaaaacg caggcgtcat
    36721 catccatatt gaaatagaaa taaacttcca tccggcgctc gtgggtgtga cacggcatgg
    36781 tgttccacaa gttacctgga gccagctccg tcagccccat actcaattgg caggtttcca
    36841 gcacatccgg gacaaaatat ttgttaatcg tgcgacggtt actggtgagg ttatcgccta
    36901 acgtgactgg agatacttcg tccggtgtga cttttttggt gggatacgtc gtatgcgcgg
    36961 gtgcgcaatt gtaataaaac ttcgccggag tggcggtatc aatactggca aagacaactt
    37021 cttttgcacc tttaccaaca tacagggcgt cgcggtgacc gatttcatag cattgaccat
    37081 cgacagtaat cgtaccggca ccgccaatat tgataacacc taactcgcga cgttcaagga
    37141 aatagcttac gccgagttgt ttaccaactt ccccgccaac ggaaaccgtt ttagttaccg
    37201 gcataatacc gccaacaata atacggtcaa tgtggctgta aaccatggtg tactcatcgg
    37261 cgacaaatac cttttcaacc aaaaattcat tgcgcagccc ctgggtatcc agcgtttttg
    37321 cgtgcgcact gtggatgctc tgtcttacgt ccacatcaac ctccgataat cagtgaagcg
    37381 tgaaaataga aaacaataat aaaacactgt ttcgttttct ctatgagggg atgatatgcc
    37441 catgggatag ggttttcaat taaaaataaa acaatgtttc attttcaagt taaggattaa
    37501 aaaaagtgca gtgtcgatca cgaatatgtc atgagaatgg aaaaatcagg gagccctgaa
    37561 aaggtatgtc gactcaccag ccatcattat tcaacagatt aatgacggca gatatttatc
    37621 ggtaaggagg aaatgcagag ctactcgtca cgttcaatgg tcaatgccac accctggcct
    37681 ccgccgatac aaagcgttgc cagtcctttg cgggcattac gtttcaccat ttcatgaaca
    37741 agagaaacca ggattcggca accggaagcg cctatcgggt gaccgagtgc gatcgcacca
    37801 ccattaacat tgacccgacg ctcatcccat tcaagcatct tgccaaccga aagcgcctgt
    37861 gcagcaaacg cttcattagc ctcgataaga tcgacatcag ccaactgcca gccaacacgc
    37921 tccaggcaac ggcgggtcgc atacaccggc gcaattccca tcaatgccgg atccacacca
    37981 acgctggcaa atgcgcggat gcgggccagc acgggtaaat tcaacgctcg tgctttggct
    38041 tcgctcatca tcattactgc agctgcccca tcgtttatgg atgatgcatt acccgctgtc
    38101 acagaaccga gactatcaaa tgaaggattt aaacgggcta agccttctgc gctggtgtca
    38161 gtgcgtggct gttcatcggt atcaacaacc aacgtctgac cgttactttg ggttattacc
    38221 gggacgatct catctttaaa tcgtccggcg tcaatcgccg ctcgcgcttt ttgttgcgag
    38281 ctaagtgcgt aagcatcctg caactgacgg ctgatgccat attcgcgagc cagattttcg
    38341 gcggtgacac caatatgata atcattgaag gcatcccaca acccatcatg cacaagacta
    38401 tcaaccaact ggctattgcc aagctgtgcg ccggtgcggc tatcagtcag aacatgtggt
    38461 gcgcggctca tgttttcctg gccaccggcg atgacaatat cagcctcgcc acactgtatc
    38521 gcctgagtag ccagatgcag tgctttaagc ccggaaccac aaacgtcatt aatagtgatt
    38581 gcagaaacgc tattaggaag accaccttta atagccgatt gccttgccgg attctgccct
    38641 gcccctgcag tcaacacctg accaagaatc acttcatcca ccgcatatgc aggaacgccg
    38701 gtacgttcta ttaacgcttt gacgaccagg ctgccaagtt ccacggcgga atgacccgct
    38761 aacgcaccac gaaagcagcc gataggtgtc cgtaacgccc cgacaatcac aacgtctttc
    38821 atcacaacct cgcaccaatt aacagtgcaa tagtaaataa ttgttaatta cagttatctt
    38881 aattgtttaa aaaaagtgac tattatcaca aaggaaatac gcctgagtag tagtcagaga
    38941 cataactggc acgtaaggtt tgcaaccact aactcaccaa tagagaggta aatagggcgt
    39001 taatctccca tacttaacct ggtttatggt aaattgccct ccattttgtt taatttgtag
    39061 atgatacgtt cagataatgt ctaatatttg gtcaaaagaa gaaactctgt ggagtttcgc
    39121 gctctacggc acagccgttg gtgcaggcac gctcttcctt cctattcagt taggttcggc
    39181 gggggctgtg gtcctgttta ttactgctct ggtcgcctgg cctttaacat attggccaca
    39241 taaagcctta tgccagttca tcctctcatc gaaaacatca gcaggtgaag ggataacggg
    39301 cgcggtaaca cactactatg gcaagaagat tggtaatctg attaccacgc tgtacttcat
    39361 cgcctttttt gtcgtcgtgt tgatatatgc agtggcaatt accaactcac ttacgaaaca
    39421 gctggcaaag catatggtta ttgatcttcg catccgtatg ttggtgagtc tgggggttgt
    39481 attaatcctg aatctcattt ttctgatggg acgccatgca accattcggg taatgggatt
    39541 tttggtattc ccattgattg cctatttctt atttctttct atttacctgg tcggtagttg
    39601 gcaacctgat ctattaacaa cccaggtaga gttcaatcag aatacccttc accagatatg
    39661 gaaatcgatt cccgtgatgg ttttcgcttt tagccatacg cccattattt ctacgtttgc
    39721 catagacaga cgtgaaaaat atggcgaaca tgctatggat aaatgcaaaa aaattatgaa
    39781 agtcgcttat ctcatcatct gcataagtgt attgttcttt gtctttagct gcctgctttc
    39841 tattccacct tcgtatattg aagcggctaa agaagaaggg gttaccattt tatcggcgct
    39901 ttctatgctg ccgaacgccc cagcatggtt atcaatttcc gggattattg tcgcagtagt
    39961 tgcgatgtcg aaatcattcc tgggtacgta ctttggcgtt attgaaggtg ccacagaggt
    40021 cgtcaaaaca acattacagc aggttggtgt aaagaaaagt cgtgcattta accgcgcact
    40081 atcaattatg ttggtatcgc tgattacctt cattgtttgt tgcattaacc cgaacgcgat
    40141 ttcgatgatt tacgcgatca gcggcccgct cattgccatg atacttttca tcatgcctac
    40201 gctgtcaacg tatctcatcc cggcgcttaa accctggcgt tccatcggaa atctgattac
    40261 gctgatcgtg ggtatcctgt gcgtatcggt aatgttcttt agctaatatc cgttgtggat
    40321 ggttcaaaat tgcttcaatt ttgaaccatc ccagttttca taatgttcat ttgttattat
    40381 tccgccctat cacttctctc gtaatattat ataattatca gctagttaaa cacacaaaga
    40441 gttctgaaca tcttaaataa gactatttaa gatacatgac gattgcattg cgtaaacgct
    40501 ttttatttac aaaaaaatgg ggaagtatta cggcgattgc gaaaagtctg aagcgcttga
    40561 attaagataa tatagaaaat cagatacaac aaaaatggca ggtagatgat gttaagataa
    40621 attggagcgg gcgaagggaa tcgaaccctc gtatagagct tgggaagctc tcgttctacc
    40681 attgaactac gcccgcttcg agatgcgtaa ggcattataa accttacgct ctccttagca
    40741 agtaccacgc tgctgactgc tgattaattc gccatcagca ttttggcttg ctgccctgag
    40801 gcggcaagta acgtagcgga tcaattgccg ttgcacggta acgaatctgg aaatgcaggc
    40861 gaacagatgc cgcatcagtg ctccccatag tggcgatttt ttgcccagct ttcacgcttt
    40921 gcccattatt taccagcatc gtgtcattat gagcgtaagc cgtaatgtaa tcttcactgt
    40981 gtttaatcat gatgagatta ccgtagccac gcagctggtt gcccacatac accacctttc
    41041 ctgcacccgc ggcgtaaata ggtgtacccc gtggagctga gatatcaatc cctttattgc
    41101 cgccatctgc tgtcgaatac ggcatgataa ctttccctgt cgttggccat aaccaacaac
    41161 gttgccctac tggcggccag gaagatttcg gtaccgctga tgacggtgta accgatgcgg
    41221 ttttggtcgt tgatttggcg gttgatttac gtgtactact gctacttttc gccccaccca
    41281 gttttagttt ctgacctact tcaatggtgt aaggggggga aatgccgttc agtcgcgcca
    41341 gttcttttac gctggttccc gtggtgcgcg aaatacgata tagcgtatcc ccccgtttca
    41401 cggtgtaaac ggagccggaa tacgttcctg tatcggatga tttgctaccc gaacagcccg
    41461 ccaaaagcag tccaaccgat aacaacatca cgatacccag agatttttta ttcaggcgtc
    41521 ccgcactcaa aatctttcct cgcttaaaaa acaagacgcc atatcatagc agccggcttc
    41581 tggataccca atctcttgct tctggaaaag cgatgtaata gagaaagagg ttaatttcat
    41641 ttggcaaagg gaagtatcat cagcaaaata tttgctgtag tggtatcgaa gttcctgact
    41701 tttaccctat ttcccacctt gatgcgtaat aatcctaatt tatcacattg attttatcgc
    41761 cactcatatt gatacgtatc actgtcccta ttcagagtcc tgtttcgtat catatctgtg
    41821 tttttttaac gtcatcacac ttccttcctc ccctcacgca tatagtcgtt tctggcgtaa
    41881 ttcttgcctg ctaagactaa atctttgcca taagaaccaa atgttcaaac cgaggggatt
    41941 tctatggaag cgcgggaagc aaccgctacg ggtgaatcat gcatgcgcgt cgatgccatt
    42001 gctaaggtca ccgggcgggc acgatatact gacgattatg ttatggcggg catgtgttac
    42061 gcgaaatatg tacgtagtcc tatcgcacat ggttatgccg taagtattaa tgatgaacaa
    42121 gccaggagtt tgccgggcgt cctggcgatt tttacctggg aagatgtgcc agaaatccca
    42181 ttcgccacgg cagggcatgc ctggacactt gacgaaaaca agcgcgatac cgccgatcgc
    42241 gcactgctaa ctcgccatgt tcgtcatcat ggcgacgccg ttgccatcgt cgtggcccgc
    42301 gatgaactca cggcagaaaa agcggcgcaa ttggtcagca ttgagtggca agaattaccc
    42361 gttatcacct cgccagaagc ggcgctggca gaagacgctg caccaatcca taacggtggc
    42421 aatttactga aacaaagcac gatgtcgacg ggtaatgtcc aacaaacaat cgatgccgcc
    42481 gactaccagg tacaggggca ctatcagact cccgttattc aacattgtca tatggaaagc
    42541 gtgacatcgc tggcatggat ggaggatgac tcgcgaatta ccatcgtttc cagcacccag
    42601 atcccgcaca ttgttcgccg cgtggttggt caggcgctgg atattccctg gtcatgcgta
    42661 cgagtcatca aaccgtttat cggtggcggt tttggtaata aacaggatgt actggaagag
    42721 ccaatggcgg cattcctgac cagcaaactt ggcggcattc cggtgaaagt ttcccttagc
    42781 cgtgaagagt gtttcctcgc aacccgtacc cgccacgctt ttactattga cgggcaaatg
    42841 ggcgtgaacc gcgacggaac attgaaaggt tatagtctgg atgttctgtc taacaccggc
    42901 gcttatgcat ctcacgggca ctccattgct tctgctgggg ggaataaagt cgcttacctt
    42961 tatcctcgtt gtgcctacgc ttacagttca aagacctgct ataccaacct cccctcggct
    43021 ggtgcgatgc gtggttatgg cgcgccacaa gtcgtatttg ccgttgagtc tatgcttgat
    43081 gatgccgcga cagcgttagg tattgatcct gttgaaattc gtttacgcaa cgccgcccgc
    43141 gaaggagatg ctaatccgct cacgggaaaa cgtatttaca gcgcagggtt gccggagtgt
    43201 cttgaaaaag gccggaaaat ctttgaatgg gaaaaacgcc gtgcagagtg ccagaaccag
    43261 caaggcaatt tacgtcgtgg cgttggcgtc gcctgtttta gctacacctc taacacctgg
    43321 cctgtcggcg tagaaatagc aggcgcgcgc ctgttgatga atcaggatgg aaccatcaac
    43381 gtgcaaagcg gcgcgacgga aatcggccag ggtgccgaca ccgtgttctc gcaaatggtg
    43441 gcagaaaccg tgggagttcc ggtcagcgat gtccgcgtaa tttcgacaca agacaccgac
    43501 gttacaccat tcgaccccgg cgcatttgcc tcacgtcaga gctatgttgc cgcgcctgcg
    43561 ctgcgcagtg cggcactgtt attaaaagag aaaatcatcg ctcacgccgc agtcatgcta
    43621 catcagtcag cgatgaatct gaccctgata aaaggccata tcgtgctggt tgaacgaccg
    43681 gaagagccgt taatgtcgtt aaaagatttg gcgatggacg ctttctacca ccctgaacgc
    43741 ggcgggcagc tctctgccga aagctccatc aaaaccacca ctaacccacc ggcgtttggc
    43801 tgtacctttg ttgatctgac ggtcgatatt gcactgtgta aagtaaccat caaccgcatc
    43861 ctcaacgttc atgattcggc tggcagaagg tcaggtacac ggcggaatgg ggatgggcat
    43921 tggctgggcg ctatttgaag agatgatcat cgatgcgaaa agcggcgtgg tccgtaaccc
    43981 caatctgctg gattacaaaa tgccgaccat gccggatcta ccacaactgg aaagcgcgtt
    44041 cgtcgaaatc aatgagccgc aatccgcata cggacataag tcactgggtg agccacccat
    44101 aattcctgta gccgctgcta ttcgtaacgc ggtgaagatg gctaccggtg ttgcaatcaa
    44161 tacactgccg ctaacgccaa aacgattata tgaagaattc catctggcag gattgatttg
    44221 aggataacat catgtttgat tttgcttctt accatcgcgc aaccaccctt gccgatgcca
    44281 tcaccctgct ggctgacaat ccgcaggcca aattgcttgc cggtggcact gacgtactga
    44341 tacagcttca ccatcacaat gaccgctatc gccatattgt tgatatccac aatctggcag
    44401 agctgcgggg aataacacag gcggaagatg gcgcgctgcg aatcggctcc gcgacaacat
    44461 ttactcagct cattgaagat cccgtaatcc aacgcaatct cccggcgtta tgtgctgcgg
    44521 ctgcatcaat cgccgggccg cagatccgta atgtcgccac ctacggcgga aatatttgca
    44581 acggtgccac cagcgcagat tctgccacgc caacgctaat ttatgacgcg aaactggaga
    44641 tccactcccc gcgcggtgtt cgtttcgtcc cgattaatgg ctttcacacc gggccgggca
    44701 aagtgtctct tgagcatgac gaaatccttg tcgcctttca ttttccgcca cagccgaaag
    44761 aacacgcggg cagcgcgcat tttaaatatg ccatgcgcga cgcaatggat atttcaacaa
    44821 ttggctgcgc tgcacattgc cgactggata acggcaattt cagcgaatta cgcctggcat
    44881 ttggtgttgc cgcgccaacg ccgattcgct gccaacatgc cgaacagact gcacaaaatg
    44941 cgccattaaa cctgcaaacg ctggaagcca tcagcgaatc agtcctgcaa gatgtcgccc
    45001 cgcgttcttc atggcgggcc agtaaagagt ttcgtctgca tctcatccag acgatgacca
    45061 aaaaagtgat tagcgaagcc gtcgccgcgg cggggggaaa attgcaatga atcacagcga
    45121 aacaattacc atcgaatgcg tcattaacgg gatgcctttt cagcttcacg ccgcgccagg
    45181 aatgccgctt tcggaactac tccgagaaca agggcttctt agtgtcaaac aaggttgctg
    45241 cgtaggcgaa tgcggtgcct gtacggtgct ggtcgacggc actgcgatag acagttgctt
    45301 attccttgcg gcctgggctg aaggaaaaga gatccgcacg ctggaaggtg aagcgaaagg
    45361 cggtaaactt tctcatgtcc aactggctta tgcgaaatcc ggcgcagtgc aatgcgggtt
    45421 ttgtacgccg ggcctgatta tggctaccac ggcgatgctg gcaaaaccac gcgaaaaacc
    45481 attaaccatt acggaaattc gtcgtggact ggcgggaaat ctttgtcgct gcacggggta
    45541 tcagatgatt gtaaatacag ttctggattg cgagaaaacg aagtaaaagg atatccggcc
    45601 tgaattcagg ccggattctc agaggttatg tgtttaacaa ctcatatttc ttaatcttgc
    45661 gatagagcgt agcaatgccg atgcccagtt catcagcaac ttccttcttg ctgttatgac
    45721 gtgaaagcgc ctcgcggatc atttgctttt ccatctcctc cagcgccgtg ccgcccgcat
    45781 catcgagtga caggtgcgcc tcactgacct ctgttacatc actttgctcc gttgtgccat
    45841 tattcagcag atttggcggc aatagcgtgc tgtcgataac ttcacctgaa ggaacaacgt
    45901 taaccagata ttccatcaaa ttgcttaact cgcgcaggtt tccgggccaa cgatgcttac
    45961 gcaatatttc gacgacatca ggagcaatgc caggataaac cgatcccaga cgacgggtat
    46021 gcagatgtaa aaagtaatgc accagtaatt caatatcttc ctgacgttca cgcagcggtg
    46081 gaagagttat cgggataaca ttaagtcggt agaagagatc ttcgcggaat ttaccttcgg
    46141 caatgaactg ggccaaattc tgattagttg cagaaatgat gcggatgtcg a
//
LOCUS       DAZGJT010000028        44754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZGJT010000028 DAZGJT010000000
VERSION     DAZGJT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.028"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..1163
                     /locus_tag="ACHKFK_001724"
     CDS             48..1163
                     /locus_tag="ACHKFK_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO9052083.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGEHIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGENQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNYD
                     FFGLVDGLDFAIQYQGKNSNRSTKKQNGDGYALSVDYNINGFGIVGAYSKSDRTNDQV
                     ADGNGSNAELWSLAAKYDANNVYAAVMYGETRNMTPGSIDTGVADREGNTIMRDQLIN
                     ETQNFEAVVQYQFDFGLRPSLGYVYSKGKDIKGVPGHRYVDADRVNYIEVGTWYYFNK
                     NMNVYTAYKFNMLDKDDAAITGAAADDQFAVGIVYQF"
     gene            1757..2608
                     /gene="hchA"
                     /locus_tag="ACHKFK_001725"
     CDS             1757..2608
                     /gene="hchA"
                     /locus_tag="ACHKFK_001725"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO9052084.1"
                     /translation="MTVQKSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESEDVAAALQWAIENDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(2715..4073)
                     /gene="hprS"
                     /locus_tag="ACHKFK_001726"
     CDS             complement(2715..4073)
                     /gene="hprS"
                     /locus_tag="ACHKFK_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO9052085.1"
                     /translation="MKRLSITVRLTLLFILLLFVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSNGMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNNEVENLLDYLEYLSDEKEICF
                     KVECNQHIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGSLNIDIASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(4073..4744)
                     /gene="hprR"
                     /locus_tag="ACHKFK_001727"
     CDS             complement(4073..4744)
                     /gene="hprR"
                     /locus_tag="ACHKFK_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO9052086.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YVLIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSSLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLISTIRGMGYSF
                     VAVKK"
     gene            4877..5290
                     /gene="hiuH"
                     /locus_tag="ACHKFK_001728"
     CDS             4877..5290
                     /gene="hiuH"
                     /locus_tag="ACHKFK_001728"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO9052087.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5399..6403
                     /gene="msrP"
                     /locus_tag="ACHKFK_001729"
     CDS             5399..6403
                     /gene="msrP"
                     /locus_tag="ACHKFK_001729"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO9052088.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKALEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGSP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            6404..7039
                     /gene="msrQ"
                     /locus_tag="ACHKFK_001730"
     CDS             6404..7039
                     /gene="msrQ"
                     /locus_tag="ACHKFK_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO9052089.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWVINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            7297..7947
                     /gene="zinT"
                     /locus_tag="ACHKFK_001731"
     CDS             7297..7947
                     /gene="zinT"
                     /locus_tag="ACHKFK_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO9052090.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDTQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(8617..8706)
                     /locus_tag="ACHKFK_001732"
     tRNA            complement(8617..8706)
                     /locus_tag="ACHKFK_001732"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8670..8672),aa:Ser,seq:cga)
     gene            8800..9597
                     /gene="mtfA"
                     /locus_tag="ACHKFK_001733"
     CDS             8800..9597
                     /gene="mtfA"
                     /locus_tag="ACHKFK_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO9052091.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            9698..9773
                     /locus_tag="ACHKFK_001734"
     tRNA            9698..9773
                     /locus_tag="ACHKFK_001734"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9731..9733,aa:Asn,seq:gtt)
     gene            9935..11197
                     /locus_tag="ACHKFK_001735"
     CDS             9935..11197
                     /locus_tag="ACHKFK_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9052092.1"
                     /translation="MSLTDAKIRTLKPSDKPFKVSDSHGLYLRVKPGGSRHWYLKYRI
                     SGKESRIALGAYPAISLSDARQQREGIRKMLALNINPVQQRAAVRGSRTPEKVFKNVA
                     LAWHKSNRKWSQNTADRLLASLNNHIFPVIGNLPVSELKPRHFIDLQKGIEEKGLLEV
                     ASRTRQHLSNIIRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGA
                     YHQGRELTRHAVLLMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVHYSGRG
                     AKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYD
                     TKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAM
                     MQWWSDYLEACRESYAPPYTIGKNKFIP"
     gene            complement(11391..12695)
                     /gene="ybtS"
                     /locus_tag="ACHKFK_001736"
     CDS             complement(11391..12695)
                     /gene="ybtS"
                     /locus_tag="ACHKFK_001736"
                     /EC_number="4.2.99.21"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salicylate synthase
                     Irp9/YbtS"
                     /protein_id="HFO9052093.1"
                     /translation="MKISEFLHLALPEEQWLPTISGVLRQFAEEECYVYERQPCWYLG
                     KGCQARLHINADGTQATFIDDAGEQKWAVDSIADCARRFMAHPQVKGRRVYGQVGFNF
                     AAHARGIAFNAGEWPLLTLTVPREELIFEKGNVTVYADSADGCRRLCEWVKEAGTTTQ
                     NAPLAVDTALNGEAYKQQVARAVAEIRRGEYVKVIVSRAIPLPSRIDMPATLLYGRQA
                     NTPVRSFMFRQEGREALGFSPELVMSVTGNKVVTEPLAGTRDRMGNPEHNKAKEAELL
                     HDSKEVLEHILSVKEAIAELEAVCQPGSVVVEDLMSVRQRGSVQHLGSGVSGQLAENK
                     DAWDAFTVLFPSITASGIPKNAALNAIMQIEKTPRELYSGAILLLDDTRFDAALVLRS
                     VFQDSQRCWIQAGAGIIAQSTPERELTETREKLASIAPYLMV"
     gene            complement(12723..14003)
                     /gene="ybtX"
                     /locus_tag="ACHKFK_001737"
     CDS             complement(12723..14003)
                     /gene="ybtX"
                     /locus_tag="ACHKFK_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin-associated zinc MFS transporter
                     YbtX"
                     /protein_id="HFO9052094.1"
                     /translation="MSDVQSNVKPLTLTTGRVIFAIAGVYVTQSLVSALSMQSLPALV
                     RAAGGSLALAGATTLFMLPWALKFIWAPWIERWRLPPGSQERRSRMLILRGQVALAAI
                     LTIAAAIGWFGREGGFPDTQIVALFVLFMVAGTVASTIDIASDGFCVDQLTRTGYGWG
                     NSVQVGGSYLGMMCGGGVFLMLSAASGWPVAMLMMAVLIMALSLPLWRITEPTRTATI
                     PHVPALGYALRRKQARLGLLLVLMLNSGMRFVLPLLAPLLLDHGLSMSALGALFSGGN
                     IAAGIAGTLAGGLLMKYTSPGRALLTAYGVQGIALLAVVMTLMMAPGHLLLQILQCLV
                     IVQSISLACALVCLYATLMSLSSPLQAGVDFTLFQCTDAAIAILAGVIGGVVAQHFGY
                     AACFLFAGAFTLLAAWVAYIRLHSARELMTSAID"
     gene            complement(13996..15798)
                     /gene="ybtQ"
                     /locus_tag="ACHKFK_001738"
     CDS             complement(13996..15798)
                     /gene="ybtQ"
                     /locus_tag="ACHKFK_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtQ"
                     /protein_id="HFO9052095.1"
                     /translation="MKDNNPADNLAWRVIWRQLISSVGSQARMLRRSMLALLLAAFMQ
                     GIAFACLYPIIDALLRGDAPQLLNWAMAFSVAAIVTLVLRWYGLGFEYRGHLAQATHE
                     LRLRLGEQLRRVPLEKLQRGRAGEMNALLLGSVDENLNYVIAIANILLLTIVTPLTAS
                     LATLWIDWRLGLVMLLIFPLLVPFYYWRRPAMRRQMQTLGEAHQRLSGDIVEFAQGMM
                     VLRTCGSDADKSRALLAHFNALENLQTRTHRQGAGATMLIASVVELGLQVVVLSGIVW
                     VVTGTLNLAFLIAAVAMIMRFAEPMAMFISYTSVVELIASALQRIEQFMAIAPPPVAE
                     QSEMPERYDIRFDNVSYRYEEGDGHALNHVSLTFPAASMSALVGASGAGKTTVTKLLM
                     RYADPQQGQISIGGVDIRRLTPEQLNSLISVVFQDVWLFDDTLLANIRIARPQATRQE
                     VEEAARAAQCLEFISRLPQGWLTPMGEMGGQLSGGERQRISIARALLKNAPVVILDEP
                     TAALDIESELAVQKAIDNLVHNRTVIIIAHRLSTIAGAGNILVMEEGQVVEQGTHAQL
                     LSHHGRYQALWQAQMAARVWRDDGGSASGEWVHE"
     gene            complement(15785..17497)
                     /gene="ybtP"
                     /locus_tag="ACHKFK_001739"
     CDS             complement(15785..17497)
                     /gene="ybtP"
                     /locus_tag="ACHKFK_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin ABC transporter
                     ATP-binding/permease protein YbtP"
                     /protein_id="HFO9052096.1"
                     /translation="MALAGLAALTSLGALLFLAWSLRDIRATPDAIPAWPLGGVIGCV
                     VLTFVLRLQAFNTSHYAAFHLENILRSRLARKALQLPPGVLQQMGSGSVAKVMLDDVK
                     SLHIFVADSTPLYARAIIMPLATIVILFWLDWRLAIATLGVLAFGSVVLVLARQRSEN
                     MAQRYHKAREQVSAAVIEFVQAMPVVRTFDSGSTSFLRYQRALEEWVDVLKTWYRKAG
                     FSARFSFSILNPLPTLFVLIWSGYGLLHYGSFDFIAWVAVLLLGSGMAEAVMPMMMLN
                     NLVAQTRLSIQRIYQVLAMPELSLPQSDQQPQEASITFEQVSFHYPQARTGAALQEVS
                     FHVPAGQIVALVGPSGAGKSTVARLLLRYADPDKGHIRIGGVDLRDMQTDTLMKQLSF
                     VFQDNFLFADTIANNIRLGAPDTPLEAVIAAARVAQAHDFISALPEGYNTRVGERGVF
                     LSGGQRQRITIARALLQDRPILVLDEATAFADPENEAALIKALAAAMRGRTVIMVAHR
                     LSMVTQADVILLFSDGQLREMGNHTQLLAQGGLYQRLWQHYQQAQHWVPGGTQEEVVE
                     NERQ"
     gene            17754..18713
                     /gene="ybtA"
                     /locus_tag="ACHKFK_001740"
     CDS             17754..18713
                     /gene="ybtA"
                     /locus_tag="ACHKFK_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin transcriptional regulator YbtA"
                     /protein_id="HFO9052097.1"
                     /translation="MTESPQTQSEISIHQLVVGKPANDGNIPAQCELLRCSLQEGMDI
                     LLWRGHFARPETLQLHDDLGRINFSCILEGTSRFAIQGLRRHTDWELARNRHYITHTP
                     DCRGSASYCGRFESITLSFSPETLALWVPDISAVIKNKIDSHCCCQQQRCNAETHLTA
                     QALRHALMRMHGGFSHEQKPSTLWLQGQSLVMLSLVLDEHREDASCLSCHFNPMERQK
                     LLRAKDLLLADLTQAPGVAELARESGLSVLKIKRGFRVLFNNSVYGLFQAERMQEARR
                     RLANGNTSVMTVAADLGYANASHFSAAFQKQFGVTPSTFKRGM"
     gene            18904..25011
                     /gene="irp2"
                     /locus_tag="ACHKFK_001741"
     CDS             18904..25011
                     /gene="irp2"
                     /locus_tag="ACHKFK_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin non-ribosomal peptide synthetase
                     HMWP2"
                     /protein_id="HFO9052098.1"
                     /translation="MISGAPSQDSLLPDNRHAADYQQLRERLIQELNLTPQQLHEESN
                     LIQAGLDSIRLMRWLHWFRKNGYRLTLRELYAAPTLAAWNQLMLSRSPENAEEETPPD
                     ESSWPNMTESTPFPLTPVQHAYLTGRMPGQTLGGVGCHLYQEFEGHCLTASQLEQAIT
                     TLLQRHPMLHIAFRPDGQQVWLPQPYWNGVTVHDLRHNDAESRQAYLDALRQRLSHRL
                     LRVEIGETFDFQLTLLPDNRHRLHVNIDLLIMDASSFTLFFDELNALLAGESLPAIDT
                     RYDFRSYLLHQQKINQPLRDDARAYWLAKASTLPPAPVLPLACEPATLREVRNTRRRM
                     IVPATRWHAFSNRAGEYGVTPTMALATCFSAVLARWGGLTRLLLNITLFDRQPLHPAV
                     GAMLADFTNILLLDTACDGDTVSNLARKNQLTFTEDWEHRHWSGVELLRELKRQQRYP
                     HGAPVVFTSNLGRSLYSSRAESPLGEPEWGISQTPQVWIDHLAFEHHGEVWLQWDSND
                     ALFPPALVETLFDAYCQLINQLCDDESAWQKPFADMMPASQRAIRERVNATGAPIPEG
                     LLHEGIFRIALQQPQALAVTDMRYQWNYHELTDYARRCAGRLIECGVQPGDNVAITMS
                     KGAGQLVAVLAVLLAGAVYVPVSLDQPAARREKIYADASVRLVLICQHDASAGSDDIP
                     VLAWQQAIEAEPIANPVVRAPTQPAYIIYTSGSTGTPKGVVISHRGALNTCCDINTRY
                     QVGPHDRVLALSALHFDLSVYDIFGVLRAGGALVMVMENQRRDPHAWCELIQRHQVTL
                     WNSVPALFDMLLTWCEGFADATPENLRAVMLSGDWIGLDLPARYRAFRPQGQFIAMGG
                     ATEASIWSNACEIHDVPAHWRSIPYGFPLTNQRYRVVDEQGRDCPDWVPGELWIGGIG
                     VAEGYFNDPLRSEQQFLTLPDERWYRTGDLGCYWPDGTIEFLGRRDKQVKVGGYRIEL
                     GEIESALSQLAGVKQATVLAIGEKEKTLAAYVVPQGEAFCVTDHRNPALPQAWHTLAG
                     TLPCCAISPEISAEQVADFLQHRLLKLKPGHTAGADPLPLMNSLAIQPRWQAVVERWL
                     AFLVTQRRLKPAAEGYQVCAGEEREDEHPHFSGHDLTLSQILRGARNELSLLNDAQWS
                     PESLAFNHPASAPYIQELATICQQLAQRLQRPVRLLEVGTRTGRAAESLLAQLNAGQI
                     EYVGLEQSQEMLLSARQRLAPWPGARLSLWNADTLATHAHSADIIWLNNALHRLLPED
                     PGLLATLQQLAVPGALLYVMEFRQLTPSALLSTLLLTNGQPEALLHNSADWAALFSAA
                     GFNCQHGDEVAGLQRFLVQCPDRQVRRDPRQLQAALAGRLPGWMVPQRIVFLDALPLM
                     ANGKIDYQALKRRHTPEAENPAEADLPQGDIEKQVAALWQQLLSTGNVTRETDFFQQG
                     GDSLLATRLTGQLHQAGYEAQLSDLFNHPRLADFAATLRKTDVPVEQPFVHSPEDRYQ
                     PFALTDVQQAYLVGRQPGFALGGVGSHFFVEFEIADLDLTRLETVWNRLIARHDMLRA
                     IVRDGQQQVLEQTPPWVIPAHTLHTPEEALRVREKLAHQVLNPEVWPVFDLQVGYADG
                     MPARLWLCLDNLLLDGLSMQILLAELEHGYRYPQQLLPPLPVTFRDYLQQPSLQSPNP
                     DSLAWWQAQLDDIPPAPALPLRCLPQEVETPRFARLNGALDSTRWHRLKKRAADAHLT
                     PSAVLLSVWSTVLSAWSAQPEFTLNLTLFDRRPLHPQINQILGDFTSLMLLSWHPGES
                     WLHSAQSLQQRLSQNLNHRDVSAIRVMRQLAQRQNVPAVPMPVVFTSALGFEQDNFLA
                     RRNLLKPVWGISQTPQVWLDHQIYESEGELRFNWDFVAALFPAGQVERQFEQYCALLN
                     RMAEDESGWQLPLAALVPPVKHAGQCAERSPRVCPEHSQPHIAADESTVSLICDAFRE
                     VVGESVTPAENFFEAGATSLNLVQLHVLLQRHEFSTLTLLDLFTHPSPAALADYLAGV
                     ATVEKTKRPRPVRRRQRRI"
     gene            25099..34590
                     /gene="irp1"
                     /locus_tag="ACHKFK_001742"
     CDS             25099..34590
                     /gene="irp1"
                     /locus_tag="ACHKFK_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin polyketide synthase HMWP1"
                     /protein_id="HFO9052099.1"
                     /translation="MDNLRFSSAPTADSIDASIAQHYPDCEPVAVIGYACHFPESPDG
                     ETFWQNLLEGRECSRRFTREELLAVGLDAAIIDDPHYVNIGTVLDNADCFDATLFGYS
                     RQEAESMDPQQRLFLQAVWHALEHAGYAPGAVPHKTGVFASSRMSTYPGREALNVTEV
                     AQVKGLQSLMGNDKDYIATRAAYKLNLHGPALSVQTACSSSLVAVHLACESLRAGESD
                     MAVAGGVALSFPQQAGYRYQPGMIFSPDGHCRPFDASAEGTWAGNGLGCVVLRRLRDA
                     LLSGDPIISVILSSAVNNDGNRKVGYTAPSVAGQQAVIEEALMLAAIDDRQVGYIETH
                     GTGTPLGDAIEIEALRNVYAPRPQDQRCALGSVKSNMGHLDTAAGIAGLLKTVLAVSR
                     GQIPPLLNFHTPNPALKLEESPFTIPVSAQAWQDEMRYAGVSSFGIGGTNCHMIVASL
                     PDALNARLPNTDSGRKSTALLLSAASDSALRRLATDYAGALRENADASSLAFTALHAR
                     RLDLPFRLAAPLNRETAEALSAWAGEKSGALVYSGHGASGKQVWLFTGQGSHWRTMGQ
                     TMYHHSTAFADTLDRCFSACSEMLTPSLREAMFNPDSAQLDNMAWAQPAIVAFEIAMA
                     AHWRAEGLKPDFAIGHSVGEFAAAVVCGHYTIEQVMPLVCRRGALMQQCASGAMVAVF
                     ADEDTLMPLARQFELDLAANNGTQHTVFSGPEARLAVFCATLSQHDINYRRLSVTGAA
                     HSALLEPILDRFQDACAGLHAEPGQIPIISTLTADVIDESTLNQADYWRRHMRQPVRF
                     IQSIQVAHQLGARVFLEMGPDAQLVACGQREYRDNAYWIASARRNKEASDVLNQALLQ
                     LYAAGVALPWADLLAGDGQRIAAPCYPFDTERYWKERVSPACEPADAALSAGLEVASR
                     AATALDLPRLEALKQCATRLHAIYVDQLVQRCTGDAIENGVDAMTIMRRGRLLPRYQQ
                     LLQRLLNNCVVDGDYRCTDGRYVRARPIEHQQRESLLTELAGYCEGFQAIPDTIARAG
                     DRLYEMMSGAEEPVAIIFPQSASDGVEVLYQEFSFGRYFNQIAAGVLRGIVQTRQPRQ
                     PLRILEVGGGTGGTTAWLLPELNGVPALEYHFTDISALFTRRAQQKFADYDFVKYSEL
                     DLEKEAQSQGFQAQSYDLIVAANVIHATRHIGRTLDNLRPLLKPGGRLLMREITQPMR
                     LFDFVFGPLVLPLQDLDAREGELFLTTAQWQQQCRHAGFSKVAWLPQDGSPNAGMSEH
                     IILATLPGQAVSAVTFTAPSEPVLGQALTDNGDYLADWSDCAGQPEQFNARWQEAWRL
                     LSQRHGDALPVEPPPVAAPEWLGKVRLSWQNEAFSRGQMRVEARHPAGEWLPLSPAAP
                     LPAPQTHYQWRWTPLNVASIDHPLTFSFSAGTLARSDELAQYGIIHDPHASSRLMIVE
                     ESEDTLALAEKVIAALTASAAGLIVVTRRAWRVEENEALSASHHALWALLRVAANEQP
                     ERLLAAIDLAENTPWETLHQGLSAVSLPQRWLAARGDTLWLPSLSPNTGCAAELPANV
                     FTGDSRWHLVTGAFGGLGRLAVNWLREKGARRIALLAPRVDESWLRDVEGGQTRVCRC
                     DVGDAGQLATVLDDLAANGGIAGAIHAAGVLADAPLQELDDHQLAAVFAVKAQAASQL
                     LQTLRNHDGRYLILYSSAAATLGAPGQSAHALACGYLDGLAQQFSTLDAPKTLSVAWG
                     AWGESGRAATPEMLATLASRGMGALSDAEGCWHLEQAVMRGAPWRLAMRVFTDKMPPL
                     QQALFNISATEKAATPVIPPADDNAFNGSLSDETAVMAWLKKRIAVQLRLSDPASLHP
                     NQDLLQLGMDSLLFLELSSDIQHYLGVRINAERAWQDLSPHGLTQLICSKPEATPAAS
                     QPEVLRHDADERYAPFPLTPIQHAYWLGRTHFIGYGGVACHVLFEWDKRHDEFDLAIL
                     EKAWNQLIARHDMLRMVVDADGQQRILATTPEYHIPRDDLRALSPEEQRIALEKRRHE
                     LSYRVLPADQWPLFELVVSEIDDCHYRLHMNLDLLQFDVQSFKVMMDDLAQVWRGETL
                     APLAITFRDYVMAEQARRQTSAWHDAWDYWQEKLPQLPLAPELPVVETPPETPHFTTF
                     KSTIGKTEWQAVKQRWQQQGVTPSAALLTLFAATLERWSRTTTFTLNLTFFNRQPIHP
                     QINQLIGDFTSVTLVDFNFSAPVTLQEQMQQTQQRLWQNMAHSEMNGVEVIRELGRLR
                     GSQRQPLMPIVFTSMLGMTLEGMTIDQAMSHLFGEPCYVFTQTPQVWLDHQVMESDGE
                     LMFSWYCMDNVLEPGAAEAMFNDYCAILQAVIAAPESLKTLASGIARHIPRRRWPLNA
                     QADYDLRDIEQATLEYPGIRQARAEITEQGALTLDIVMADDPSPSAAMPDEHELTQLA
                     LPLPEQAQLDELEATWRWLEARALQGIAATLNRHGLFTTPEIAHRFSAIVQALSAQAS
                     HQRLLRQWLQCLTEREWLIREGESWRCRIPLSEIPEPQEACPQNQWSRALAQYLETCI
                     ARHDALFSGQCSPLELLFNEQHRVTDALYRDNPASACLNRYTAQIAALCSAERILEVG
                     AGTAATTAPVLKATRNTRQSYHFTDVSAQFLNDARARFHDESQVSYALFDINQPLDFT
                     AHPEAGYDLIVAVNVLHDASHVVQTLRRLKLLLKAGGRLLIVEATERNSVFQLASVGF
                     IEGLSGYRDFRRRDEKPMLTRSAWQEVLVQAGFANELAWPAQESSPLRQHLLVARSPG
                     VNRPDKKAVSRYLQQRFGTGLPILQIRQREALFTPLHAPSDAPTEPAKPTPVAGGNPA
                     LEKQVAELWQSLLSRPVARHHDFFELGGDSLMATRMVAQLNRRGIARANLQDLFSHST
                     LSDFCAHLQAATSGEDNPIPLCQGDGEETLFVFHASDGDISAWLPLASALNRRVFGLQ
                     AKSPQRFATLDQMIDEYVGCIRRQQPHGPYVLAGWSYGAFLAAGAAQRLYAKGKQVRM
                     VLIDPVCRQDFCCENRAALLRLLAEGQTPLALPEHFDQQTPDSQLADFISLAKTAGMV
                     SQNLTLQAAETWLDNIAHLLRLLTEHTPGESVPVPCLMVYAAGRPERWTPAETEWQGR
                     INNADDAVIEASHWQIMMEAPHVQACAQHITRWLCATSTQPENTL"
     gene            34587..35687
                     /gene="ybtU"
                     /locus_tag="ACHKFK_001743"
     CDS             34587..35687
                     /gene="ybtU"
                     /locus_tag="ACHKFK_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis oxidoreductase YbtU"
                     /protein_id="HFO9052100.1"
                     /translation="MMPSASPKQRVLIVGAKFGEMYLNAFMQPPEGLELVGLLAQGSA
                     RSRELAHAFGIPLYTSPEQITRMPDIACIVVRSTVAGGTGTQLARHFLTRGVHVIQEH
                     PLHPDDISSLQTLAQEQGCCYWVNTFYPHTRAGRTWLRDAQQLRRCLAKTPPVVHATT
                     SRQLLYSTLDLLLLALGVDAAAVECDVVGSFSDFHCLRLFWPEGEACLLLQRYLDPDD
                     PDMHSLIMHRLLLGWPEGHLSLEASYGPVIWSSSLFVADHQENAHSLWRRPEILRDLP
                     GLTRSAAPLSWRDCCETVGPEGVSWLLHQLRSHLAGEHPPAACQSVHQIALSRLWQQI
                     LRKTGNAEIRRLTPPHHDRLAGFYNDDDKEAL"
     gene            35684..36487
                     /gene="ybtT"
                     /locus_tag="ACHKFK_001744"
     CDS             35684..36487
                     /gene="ybtT"
                     /locus_tag="ACHKFK_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis thioesterase YbtT"
                     /protein_id="HFO9052101.1"
                     /translation="MTQSAMCIPLWPARNGNTAHLVMCPFAGGSSSAFRHWQAEQLAD
                     CALSLVTWPGRDRLRHLEPLRSITQLAALLANELEASVSPDTPLLLAGHSMGAQVAFE
                     TCRLLEQRGLAPQGLIISGCHAPHLHSERQLSHRDDADFIAELIDIGGCSPELRENQE
                     LMSLFLPLLRADFYATESYHYDSPDVCPPLRTPALLLCGSHDHEASWQQVDAWRQWLS
                     HVTGPVVIDGDHFYPIQQARSFFTQIVRHFPHAFSAMTAWQKQPSTSER"
     gene            36491..38068
                     /gene="ybtE"
                     /locus_tag="ACHKFK_001745"
     CDS             36491..38068
                     /gene="ybtE"
                     /locus_tag="ACHKFK_001745"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="yersiniabactin biosynthesis salycil-AMP ligase
                     YbtE"
                     /protein_id="HFO9052102.1"
                     /translation="MNSSFESLIEQYPLPIAEQLRHWAARYASRIAVVDAKGSLTYSA
                     LDAQVDELAAGLSSLGLRSGEHVIVQLPNDNAFVTLLFALLRLGVIPVLAMPSQRALD
                     IDALIELAKPVAYVIHGENHAELARQMAHKHACLRHVLVAGETMSDDFTPLFSLHGER
                     QAWPQPDVSTTALLLLSGGTTSTPKLIPRRHADYSYNFSASAELCGISQQSVYLAVLP
                     VAHNFPLACPGILGTLACGGKVVLTDSASCDEVMPLIAQERVTHVALVPALAQLWVQA
                     REWEDSDLSSLRVIQAGGARLDPTLAEQVIATFDCTLQQVFGMAEGLLCFTRLDDPHA
                     TILHSQGRPLSPLDEIRIVDQDENDVAPGETGQLLTRGPYTISGYYRAPAHNTQAFTA
                     QGFYRTGDNVRLDEVGNLHVEGRIKEQINRAGEKIAAAEVESALLRLAEVQDCAVVAA
                     PDTLLGERICAFIIAQQVPTDYQQLRQQLTRMGLSAWKIPDQIEFLDHWPLTAVGKID
                     KKRLTALAVDRYRHSAQ"
     gene            38200..40221
                     /gene="fyuA"
                     /locus_tag="ACHKFK_001746"
     CDS             38200..40221
                     /gene="fyuA"
                     /locus_tag="ACHKFK_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore yersiniabactin receptor FyuA"
                     /protein_id="HFO9052103.1"
                     /translation="MKMTRLYPLALGGLLLPAIANAQTSQQDESTLVVTASKQSSRSA
                     SANNVSSTVVSAPELSDAGVTASDKLPRVLPGLNIENSGNMLFSTISLRGVSSAQDFY
                     NPAVTLYVDGVPQLSTNTIQALTDVQSVELLRGPQGTLYGKSAQGGIINIVTQQPDST
                     PRGYIEGGVSSRDSYRSKFNLSGPIQDGLLYGSVTLLRQVDDGDMINPATGSDDLGGT
                     RASIGNVKLRLAPDDQPWEMGFAASRECTRATQDAYVGWNDIKGRKLSISDGSPDPYM
                     RRCTDSQTLSGKYTTDDWVFNLISAWQQQHYSRTFPSGSLIVNMPQRWNQDVQELRAA
                     TLGDARTVDMVFGLYRQNTREKLNSAYDMPTMPYLSSTGYTTAETLAAYSDLTWHLTD
                     RFDIGGGVRFSHDKSSTQYHGSMLGNPFGDQGKSNDDQVLGQLSAGYMLTDDWRVYTR
                     VAQGYKPSGYNIVPTAGLDAKPFVAEKSINYELGTRYETADVTLQAATFYTHTKDMQL
                     YSGPVGMQTLSNAGKADATGVELEAKWRFAPGWSWDINGNVIRSEFTNDSELYHGNRV
                     PFVPRYGAGSSVNGVIDTRYGALMPRLAVNLVGPHYFDGDNQLRQGTYATLDSSLGWQ
                     ATERMNISVYVDNLFDRRYRTYGYMNGSSAVAQVNMGRTVGINTRIDFF"
     gene            40872..41621
                     /locus_tag="ACHKFK_001747"
     CDS             40872..41621
                     /locus_tag="ACHKFK_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
                     /protein_id="HFO9052104.1"
                     /translation="MSKKYQPLLITHYMSTWVTITEAVEITTKAIKQKITPSDIYRHA
                     LSGNILLSVYFQSPVILKKIQTFNGKIKFRQFVGDLLDKLCMLDRDGFIYGQNLRLCT
                     EARYICPVQQIIDTPLLRKLNQFRTFVRNVRPGDELDVPAQVSEKNLTPPPGNSSGNL
                     EQQIASTSQLIGSLLAEDMNSEQAANIARGWASSQASGVMTDWLSRFGTARITLGVDE
                     DFSLKNSRDGNPRKWRFDATNSELCAERHEC"
     gene            41548..43670
                     /locus_tag="ACHKFK_001748"
                     /pseudo
     CDS             41548..43670
                     /locus_tag="ACHKFK_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            complement(43840..>44754)
                     /locus_tag="ACHKFK_001749"
     CDS             complement(43840..>44754)
                     /locus_tag="ACHKFK_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO9052105.1"
                     /translation="VIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQD
                     HATLKEIKDLAKNITLQYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCY
                     SPLCKNASRMDQNLRTPVPMTWAFMMREAGFPAVRSIYELPLSDDVLDEVREEMRSLG
                     SYIALNLDGSSQERTFSLSIAENLIAKIQSETDIPIVIVYGPKGEDKARALVDCYNNV
                     YRLSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVS
                     ESVVVGQKIDNISLDEFAKALKSVLARI"
BASE COUNT        10581 a        12543 c        11557 g        10073 t
ORIGIN      
        1 cacttattga tatattcatg aagattataa tcacaaggga atacataatg aaaagaaaag
       61 ttctggcaat gctggtcccg gcgttattag ttgctggcgc agcaaatgcg gctgaaattt
      121 ataataaaga tggcaataaa gttgatttct acggcaagat ggttggcgaa cacatctggt
      181 caaatactga tgataataac agcgaaaacg aagatacctc ctatgctcgt ttcggtgtta
      241 aaggtgaaac ccaaatcacc agcgaactga ctggtttcgg tcagtttgaa tacaacctcg
      301 acgccagcaa acctgaaggt gaaaaccagg aaaaaacccg tttaaccttc gccggtttga
      361 aatataacga gttaggatca tttgactatg gccgtaacta cggtgttgct tatgatgccg
      421 cagcttatac cgatatgtta gttgaatggg gtggcgattc ctgggcttcc gctgacaact
      481 tcatgaatgg tcgtaccaac ggtgttgcaa cctaccgtaa ctatgatttc tttggcctgg
      541 ttgatggtct tgattttgcg attcagtacc aaggcaaaaa cagcaaccgt agcactaaga
      601 aacaaaatgg tgacggctat gcgttgtctg tagactataa catcaatggt tttggtatcg
      661 ttggtgcata tagcaaatct gatcgcacca acgatcaagt tgccgatgga aacggttcaa
      721 atgctgaact gtggtcattg gctgcaaagt atgacgcgaa caatgtctac gcagctgtaa
      781 tgtacggtga aactcgtaat atgactccgg gttctattga tacaggtgtt gccgatcgag
      841 aaggcaatac aataatgcgt gatcaactta tcaatgaaac ccagaacttt gaagcggttg
      901 ttcagtatca attcgacttt ggtctgcgtc cgtccttagg ttatgtgtat tctaaaggta
      961 aagatatcaa aggtgtacct ggacatagat atgttgacgc tgaccgtgtg aactacattg
     1021 aagttggtac ctggtactac ttcaataaga acatgaacgt ttatactgca tacaaattca
     1081 acatgctgga taaagatgac gccgccatta ctggcgcagc agctgatgac cagtttgcag
     1141 ttggtatcgt ctaccagttc taatcagcca acgcgccgcg gcgtgttact gtaaaactta
     1201 acactagcct gagttcgtcc ctcatcgaac tcaggctttt ttattggcat ttcatctcga
     1261 tctgcggcac acgcaattgc caaagcatat cgatacagca ccttaccccg cccccccctg
     1321 gctcaataaa atctaccagc gtctcttcca gccaacaccc aaatgcattt ctttataaat
     1381 ttaatctgta acaaaaccgc ttcccatata gaaatatttg caaaaatagt gcattaaaaa
     1441 agctttttcc ctaccaatcc gggatataaa tattcatcag caccataata ttccacataa
     1501 ctaaatctct ccctaccgcc ccgtacctct gataatggtc taaaatcatt gaggccactt
     1561 gcgacggcgt tgctcaataa ggaaaatttg gcgtgaataa tattcaacac cagtgcagca
     1621 tcatcattac atacttacgc ccgccgacta ccgctttcga tatattgact caattttcca
     1681 gcctggttaa aaatagcggc cagctcagtc gcaaatatag tgactaccct aactaagcaa
     1741 caataaggaa tacactatga ctgttcaaaa aagtaaaaat ccacaggtcg atattgctga
     1801 agataatgca ttcttccctt cagaatattc gcttagccaa tatacgagtc ccgtctctga
     1861 tcttgatggc gtggactatc caaaaccgta tcgcggtaaa cataaaattc tggtgatcgc
     1921 cgctgatgaa cgctatttgc cgactgataa cggcaaactc ttctcaacgg gcaatcatcc
     1981 gattgaaacg ttgctgccgt tgtatcatct ccatgctgca ggtttcgaat tcgaagtggc
     2041 gaccatttcc ggtctgatga ccaagtttga atactgggct atgccgcaca aagatgaaaa
     2101 agtaatgcca ttctttgagc agcataaatc gttgttccgc aatccgaaga aattagcgga
     2161 tgttgtcgcc agcctgaacg cagacagcga atatgcagcg atctttgttc ccggtggtca
     2221 tggtgcactc atcggtttgc cagaaagtga agacgtagcc gccgctctgc agtgggctat
     2281 tgaaaatgac cgttttgtta tctccctatg ccatggtccg gctgcttttc tggcgcttcg
     2341 ccacggcgat aacccactga acggttattc catttgtgca ttcccggacg ccgcagacaa
     2401 acaaacgcca gagataggct atatgccggg tcatctcacc tggtacttcg gcgaagagct
     2461 gaagaaaatg ggcatgaaca tcattaatga cgacatcacc gggcgagtac ataaggaccg
     2521 taaagttctc accggcgaca gtcctttcgc agcgaatgcg ttgggtaaac tggcggcaca
     2581 ggaaatgctg gcagcttacg cgggttaatc cctgttacgt ttgaatgtaa gcgcataatc
     2641 aatcgcagcc gcgttagtac tcacgacgtg gctgcgtttt tcacggttaa tttatggcgt
     2701 actgaagccc tatgttaatt tctttgcggt aacgtaatcc ggaacacatt atgcttactg
     2761 agatagtgat acgtagcact tcccccatgt aattcggcaa tcgctttgac taaagaaaga
     2821 ccaagtccct gacctacgga atgacgcgaa ttatctcccc gccaaaatct acggaagagt
     2881 ttttcaggct catgaatttt cgttccagga ctggcgatat caatattaag agaaccgttg
     2941 gtatcaagaa aactggttat ctgaatacgc gatttttctg gcgaatatcg aatagcgtta
     3001 acaataagat tcgataacat tcgttgtagt aaaattttat ccgcaaagat gtgctgattg
     3061 cactcgacct taaagcaaat ctctttctcg tctgaaaggt attcaagata gtccaacaaa
     3121 ttttcgactt cattattgag agaaagcgag tccagtttca ccaaaacatt atttttatct
     3181 gccctggcaa gaaacagtat gttctctgtt aatcgcgaaa tattttccag ctcttcaatg
     3241 tttccggcaa ttgttttttg atattcagcg atacttctgg tttgactgag cgtaacctga
     3301 ttctgaccca gtaatgcatt aattggcgtt ctaagttcat gagcgagatc gtcagcaaac
     3361 tgacttagac gttcaaaatc tttgactaaa gcttggtgca ttttattcaa cgcctgccca
     3421 agaggtttta gttctctcgg taatgcgcta acctcaacag gctctcggct atcgttataa
     3481 ttcagcgctt ccgttacacc actcaacttt ttgatctctc gtaaccccgt tctgattaac
     3541 aacggactta atactgagca cagtataatg gcgacaatgc aaattataat gctattaatt
     3601 ttatactgtt caagcatgtt atgtctggct gaagccaatt tagccacagt aaccgttaat
     3661 gatggcgaaa cttcatcaat attaattcgt aaagcatcta tctctgtgtc attaataatg
     3721 cttctgtaaa tgccagctgc gctgattgtc tcactagcag gtatgttatt taacatgcca
     3781 ttactgacat ttgtccggtt aacaatttta ttgatgccat caccatgaat gatcaagata
     3841 tcctgactaa catccatcat ccggttaaag tacacaggta acgtatctgg atttacccca
     3901 tcaattaaca actgcttgat ctgcgctgtc cggttaatga gtgttgtatc atcgcgccat
     3961 ttcaactcac ttgccaggcc attatagaga gtccagacaa ttccagcgcc agcaacaaac
     4021 agcagcaata taaaaagcaa ggttaaacgg acggttatag ataatctttt cattattttt
     4081 ttaccgctac gaatgaatag cccatccccc ggattgtgga gattagcttt tcaggaaaag
     4141 gatcatcaac ttttgcgcgg agcctgcgaa tggcgacgtc caccgtattg gtatcactat
     4201 caaagttgat tccccaaatt tcactcgcaa taaccgttct ggggataatc tcgccagctc
     4261 tggaggccag taaccaaagt aactgaaact ccttgcgcgt cagtgtaata ctgatattgt
     4321 ccctgctcac actttggctg acagagtcca ttcttaagcc gctgatttct aatgatgaat
     4381 tcagagcgtg atgttgcctt aattgtgccc gaacccttgc cagcaactca gaaaatgaaa
     4441 aaggttttac cagataatca tttgccccac tgtccagccc tctgactctg tcatcgacag
     4501 aatcccttgc agtaaggcaa ataacagggg tttgctttgc tgttcttaac gtttgtaaga
     4561 tctgccagcc atccataccc ggaagcataa tatccagaat gatcaataca taatcatcct
     4621 tcagcgcaag ataaagccca tctctgccat ctgaaacggc atcaatgaca taacccgctt
     4681 cggaaagccc ctgcgttacc cattcctggg tcctttgatt atcttcaata agtagaatct
     4741 tcatgaaaat aatatgccat aaatcatatg ttatagaaac agcctggttc attacaaaat
     4801 tgtaatgctg ctgtaaggtt accctggacg ttttttcgct atcctcaaaa ctcattcaca
     4861 tgacaaggat ataaacatgt taaagcgtta tttagtactc tccgtagtaa cggctgcatt
     4921 ttcattacct tctttggtct atgctgcaca acaaaacatt cttagcgtgc acattttgaa
     4981 ccagcaaaca ggaaaacccg ctgccgacgt gacagtcact cttgaaaaga aagcggataa
     5041 cggctggtta caacttaata ccgccaaaac agataaggat ggacgaatta aggcactgtg
     5101 gcccgagcaa actgcgacta ctggcgatta ccgtgtcgta tttaaaaccg gggactattt
     5161 caagaaacaa aatcttgaaa gtttcttccc tgagatcccc gttgaatttc atattaataa
     5221 agtgaacgag cattatcatg tgcctttgtt actcagccaa tatgggtatt caacctatcg
     5281 tggcagttaa tgcgtaaata tgtaaaagct gatctctgct actgttaggg aggtgactcc
     5341 ctgacagatt aacagtaaac ggctcttgtt ggctaacgac aaaaaagtgt gatggcttat
     5401 gaaaaagaat caatttttaa aagaatcaga tgttacggca gagtcggtat tctttatgaa
     5461 gcgtcggcag gtgttaaaag cactgggcat cagcgcggct gcactttctt tgcctcacgc
     5521 tgcgcatgcc gatctgctta gctggtttaa agggaacgat cgcccacccg cccccgccgg
     5581 aaaagcgctg gagttcagca agcctgccgc ctggcaaaat aacctgccac tgacgccagc
     5641 agataaagtt tccggttata acaacttcta tgaattcggg ctggataaag ccgatcccgc
     5701 cgctaatgct ggtagcctga aaaccgatcc atggacactg aaaatcagcg gcgaagtggc
     5761 aaaaccattg accctcgatc acgatgattt aacccgtcgc ttcccgctgg aagagcgtat
     5821 ttatcgtatg cgctgcgtgg aagcgtggtc gatggtggtg ccgtggattg gttctccgct
     5881 acacaaattg ctggcgcttg ccgaacccac cagcaatgcg aagtatgtcg ctttcgaaac
     5941 gatttatgcg ccggaacaga tgccaggcca gcaggaccgc tttatcggcg gcgggctgaa
     6001 atatccttat gtcgaaggat tgcgtctcga cgaagcaatg catccgctca cactgatgac
     6061 cgtaggtgtt tatggcaagg cgttaccacc gcaaaatggc gcgccggtgc gactgattgt
     6121 gccgtggaaa tatggcttta aagggattaa atcgatcgtc agtattaagc tgacccgcga
     6181 gcgtccgcca accacctgga atctggcagc gcctgacgaa tacggttttt acgccaacgt
     6241 taatccgcat gttgatcacc cgcgctggtc acaggctacc gaacgattta ttggttcagg
     6301 cggcattctc gatgttcagc gccagccaac actactgttt aatggttacg ccgaccaggt
     6361 ggcatcgctg tatcgtggcc tggatttgcg ggagaatttc tgaatgcgtc tgacagcaaa
     6421 acaggtgaca tggctgaaag tttgcctgca tcttgccgga ttgttgccgt ttctctggct
     6481 ggtctgggtg attaatcacg gtggactggg tgccgatccg gtgaaagata ttcagcattt
     6541 taccggtcgc actgcactga aatttttgct ggcgaccttg ttaatcacac ctctggcacg
     6601 ctacgcaaaa cagccgttat tgatacgcac tcgccgcctg ttaggattat ggtgctttgc
     6661 ctgggcgaca ctgcatttaa ccagttacgc attgctggag ctgggcgtga ataatctggc
     6721 gttattaggt aaggagttaa ttacccggcc ttatttaacg ttaggaatta tcagctgggt
     6781 tattttgctt gctttagcgt tcacttcaac ccagtcgatg cagcgaaaac tcggcaaaca
     6841 ctggcaacag ttgcataact tcgtctatct tgtcgcgatc ctggccccaa tacattatct
     6901 gtggtcagtg aagattattt caccgcagcc actcatctat gctgggctgg ctgtactgct
     6961 tttagcctta cggtataaga agttgctttc ccttttcaac cggttacgca agcaggccca
     7021 caacaaatta tctctataac ttcatattgc cgacaaactt cgcgttatcc cggccggatg
     7081 cggcgcgagc gccctatccg gcccacacag atcgcatcaa gttatgtttc gtttgtcatc
     7141 aacctttcat caccagaata atttatttta tttcaataag atagttcaat acacctgaga
     7201 ataccatgct ctcatttcct aatgagttgt tgcatttttt catatgttac aatataacat
     7261 tacacatcat gtacattaac tctggaggaa attgttttgg cgattcgtct tcataaactg
     7321 gctgttgctt taggtgtctt tattgttagc gctcctgcct tttcgcatgg tcatcattct
     7381 catggcaaac ccttaacaga ggtcgaacaa aaagctgcta atggtgtttt tgatgatacc
     7441 aatgtacaaa accgaacgct cagtgactgg gatggagtct ggcaatccgt ttacccttta
     7501 ctgcaaagtg gcaaacttga tcccgtcttt cagaagaaag cggatgcaga taaaaccaaa
     7561 acatttgctg aaattaaaga ttattatcgc aaaggttatg cgacagatat cgaaatgatt
     7621 ggcattgagg acggcattgt cgaattccat agaaataatg aaacaacatc ctgtaaatat
     7681 gattacgatg gatacaaaat actcacctat aaatcaggca agaaaggcgt tcgttactta
     7741 ttcgaatgta aagatcctga aagcaaagcc cctaaatata tacaatttag cgatcatata
     7801 attgcaccac gaaaatcatc tcattttcac atttttatgg gtaatgatac ccaacagtct
     7861 ttattgaatg aaatggaaaa ctggccaacg tattatccct atcagttgag tagcgaagaa
     7921 gtggtcgagg agatgatgtc tcattgattt ttttgcgctg acttcagtta tttaacagaa
     7981 cagtgcctgg tagtaacaac aataatttac caagacaaat aagtaagaca aactcagaca
     8041 cgctcatact tgagtcgtgt cttttttatt atgtctgtag aaaaaaactg cgaaaaaggc
     8101 gaacaataaa tacacaactc agccatacag actagcagcg caaatgtttc caggagcaag
     8161 tcatctgtca tggtaaagct aaccataaat ataggttaat tttgatgttt aaaccttttt
     8221 tacttgttag tatgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa
     8281 attatggact ttgtcccgcg gctttaatct gacggccgcg ttcctttttt ggctcaatta
     8341 ttgacccctt ctctacgcaa cttcagttcc cgccagcaac tttgcggcag tttcatagga
     8401 tcaatgtcta aaagaataat ggtgaccgat aagaaaacga ctgaataact gtagattttc
     8461 gctcgaaacc ttcctgtcag acccatagcg aatcaagtgc tgaatgtcac agtatcgaac
     8521 agaaaacagt gacgatctaa cccttcaaga atattctacg attgttctgt ttaggaaaag
     8581 caaggcggga agtcgggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac
     8641 ccccggtaga gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat
     8701 ctctccgttc agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgtcc
     8761 gtgcaatttt gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa
     8821 gtacaagaat cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt
     8881 ttaacgtgtc tgacagaaca ggaacaaagc aaattggtcg ctcttgccga acgtttttta
     8941 cagcaaaaac ggcttgttcc tttacagggc tttgagctga attcattaag aagctgccgg
     9001 atagcacttc tattttgcct gcccgttctg gagttaggac tggaatggct ggatggtttt
     9061 catgaagtct taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc
     9121 ggtctggtgc ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc
     9181 gttttgaact ggttggatat acaagattct tttgatgctt ctggttttaa cctgattatt
     9241 catgaagtcg ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt
     9301 atttcgttgc gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt
     9361 caggaagaaa tcgaattagt tggtgagaat gcggcgagca ttgatgctta tgctgccagt
     9421 gatcctgctg aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt
     9481 gctcctcgtt tcccttcatt gtggcaacgt ttctgtcaat tttatcaaca agatcctttg
     9541 cagagactgc atcacgctaa tgatacagac tcgttttcgg caacgaatgt tcattaatta
     9601 acaactttgc agattaatta accaattgaa atggcttatg aaatttagtg ttgacagaca
     9661 aggtaccgct aagtaatatg cgccccgttc tcacgattcc tctgtagttc agtcggtaga
     9721 acggcggact gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaattttct
     9781 gttttcttgc atccttgcga atctttatct gatgttgatt caacaggtta gtgtgaaaac
     9841 tcttctcggt gcattttgat tttaccctac gcatcgggaa aaattggtgg tcaaatctgg
     9901 ggtcaggtta gttcgataat ggagtgcccc ccatatatcc cttaccgacg caaaaatccg
     9961 caccctcaag ccttctgata aaccctttaa agtctccgat tctcacggtc tgtatctgcg
    10021 ggtcaagccg ggtggctccc gccactggta tctcaaatac cgtattagcg gtaaagaatc
    10081 ccgcattgcg ctgggtgcct atccagccat ctccctgtct gatgcgcgac agcaacgtga
    10141 aggtatccgt aaaatgctgg cgctgaatat caacccggta cagcagcggg ctgctgtacg
    10201 tggctcacga acaccggaga aagtttttaa aaacgtggcg ctggcatggc ataaaagtaa
    10261 caggaaatgg tcgcagaaca ccgccgaccg tctgcttgcc agcctgaaca atcacatctt
    10321 tccggtcatc gggaacctac ctgtatcaga acttaaaccc cgtcatttca ttgacctgca
    10381 gaaagggatc gaggaaaaag gtctgctgga ggttgcgtcc cgcacacggc agcacctgag
    10441 taacataata cgccatgcgg tccatcagga gttaatcgat acgaaccctg cagcaaacct
    10501 tggcggcgtg accacacctc ctgtcagacg gcactatcct gccctgccgc tggagcggct
    10561 gcctgaactg cttgaacgta ttggggcata tcatcagggc cgtgaactga cccggcatgc
    10621 cgttctgctg atgctgcatg tgttcattcg ctccagtgaa ctgcgtttcg cccgctggtc
    10681 agagattgat ttcacaaacc gagtctggac gatacccgcg acgcgagaac ccattattgg
    10741 cgtgcattat tccggccgcg gggcaaaaat gcgaatgccg catatcgtcc ccctctcaga
    10801 acagtccatc gccattctga aacagattaa ggatatcacc ggtaataatg aactgatctt
    10861 ccccggcgac cataacccgt ataagccaat gtgtgaaaac acggtcaata aggcactgcg
    10921 ggtgatgggt tacgacacga aaaaggatat ctgcggtcac ggcttccggg caatggcatg
    10981 cagtgcgctg atggaatcgg gtttatgggc aaaggacgca gtagaacgcc agatgagtca
    11041 tcaggagcgc aataccgtgc gcatggctta tattcataag gcagagcacc tggaagcccg
    11101 caaagcgatg atgcagtggt ggtcggatta tctggaagca tgccgagaat cttatgcacc
    11161 gccttataca attggtaaaa ataagtttat cccatagtat actaacgaat cccacactct
    11221 ggcaaattac caccacctcc acaggaggtg gttctatata gacttttatc aacttcatga
    11281 cgaaaaacac ctattttatc ccaaaagaag acaatagagt cacagaggaa tcaaagagcg
    11341 actcaattgt ctttggacag tctggttgtg aggttagaaa acagttactc ctacaccatt
    11401 aaatagggcg caatgctcgc taatttctcc cgggtttctg tcagttcgcg ttccggcgta
    11461 gattgcgcga tgattcccgc ccccgcctgt atccagcagc gctggctatc ctgaaatacg
    11521 gaacgcagaa ctagcgccgc atcaaagcgc gtatcgtcca gcagcaggat tgcgccggaa
    11581 taaagctctc gcggcgtctt ctcaatttgc ataatcgcgt tcagagcagc atttttaggg
    11641 atacctgagg cggtaatcga cggaaacagc acggtaaacg catcccaggc atccttgttt
    11701 tccgcaagct gaccgctcac gccagacccc agatgctgaa cgctgccgcg ctggcgaacc
    11761 gacattaaat cttcaaccac cacgctgccc ggctggcaaa cagcctccag ttcagcaata
    11821 gcttctttga cagaaaggat atgctcaagc acctctttac tgtcgtgcag cagttctgcc
    11881 tctttcgcct tattatgctc cgggtttccc atgcgatcgc gggtgcccgc aagcggttca
    11941 gtgaccactt tattgcccgt cactgacatc accagttccg ggctaaagcc cagcgcttcg
    12001 cgtccttcct gacggaacat aaacgagcgc acaggcgtgt ttgcctgccg cccgtataac
    12061 agcgtggcgg gcatatcaat ccgcgatggc aggggaatgg cgcgcgagac aatcactttg
    12121 acatactcgc cacggcggat ctccgcaacg gcgcgcgcaa cctgttgttt atatgcctca
    12181 ccattgaggg cggtatccac cgccagtggt gcgttctgcg tcgttgtacc ggcctctttt
    12241 acccactcgc acaaacgtcg gcacccgtcg gcggagtccg cataaacggt gacatttccc
    12301 ttttcaaaaa taagttcttc acggggaacg gttaacgtca gcagcggcca ctccccggcg
    12361 ttaaaggcaa tcccccgcgc atgcgccgca aagttgaacc caacctgtcc atatacccga
    12421 cgtcctttca cctgaggatg cgccataaaa cgacgcgcgc agtcggcaat ggaatccacc
    12481 gcccattttt gctccccagc atcatcaata aatgtcgcct gcgttccgtc ggcattaatg
    12541 tgcagccgtg cctggcaccc tttgcctaaa taccaacagg gttgacgctc atagacataa
    12601 cactcttctt ctgcgaactg gcgtaaaacg ccagaaatcg tcggtagcca ttgttcctct
    12661 ggtaacgcca gatgtagaaa ttcactgatt ttcatccctt acctctctgt gttattcccg
    12721 gatcaatcaa ttgcgcttgt catcagttct cttgccgaat gcagccggat ataagcaacc
    12781 cacgccgcca gcaacgtgaa tgccccggca aacaggaagc aggccgcata gccaaaatgt
    12841 tgagcaacaa cgccaccgat aacgccagcc agaatggcga ttgccgcgtc agtacattga
    12901 aagagggtga agtcgacacc ggcctgcaaa ggcgatgaaa gcgacatcag cgtggcgtaa
    12961 agacagacca gcgcgcaggc cagcgaaatg gactggacaa tgaccagaca ctggagaatc
    13021 tgcagcagca gatgacccgg cgccatcatg agcgtcatca ccaccgccag cagcgcgatc
    13081 ccctggacgc cataagccgt caacagcgct ctgccgggtg aggtgtattt catcagcaat
    13141 ccgccggcca gcgttcctgc tatgcccgct gcaatattgc cgccgctgaa cagcgcgccc
    13201 aatgcggaca tgctcaaccc atgatccaac aacagcggtg ccagaagagg cagcacaaac
    13261 cgcatgcctg aattcagcat caataccagc agtaagccca ggcgcgcctg cttcctcctt
    13321 agcgcataac ctaacgccgg aacatgcggg atagtcgctg ttcgcgtcgg ctccgtaatg
    13381 cgccacagcg ggagtgacag cgccataatc agcaccgcca tcatcagcat ggcgacaggc
    13441 cagccggatg ccgccgacag catcaggaac accccgccgc cgcacatcat tcccagatag
    13501 ctgccgccga cctgcacgct gtttccccag ccgtaacccg tgcgggtcag ttgatcgaca
    13561 caaaagccgt cgctggcgat atcaatggtg gaggcgaccg tgcctgccac cataaacaga
    13621 acaaataacg cgacgatttg cgtatcgggg aatccccctt ctcgcccaaa ccagccaatc
    13681 gctgcggcaa tagtcaggat cgccgctagc gcgacctgac cacgcaggat taacatgcgg
    13741 gaacggcgtt cctggctacc gggcggaagc cgccagcgct cgatccacgg cgcccaaata
    13801 aacttcagcg cccagggcag cataaacagg gttgtcgcac cggcaagcgc cagcgaaccg
    13861 ccagcagcgc gcaccagcgc gggtaaggac tgcatagaca gcgccgatac cagactctgc
    13921 gtcacataga cgccggcaat agcaaaaatc acccgcccgg tcgtcaacgt cagaggtttc
    13981 acattcgact gaacatcact catgcaccca ctctccagac gcggaacccc cgtcgtcgcg
    14041 ccacacgcgc gcggccattt gcgcctgcca cagcgcctga taacgtccat gatgtgagag
    14101 caattgcgca tgagtaccct gctcaaccac ctgtccctct tccatcacca gaatgtttcc
    14161 ggccccggcg atggtggata aacggtgagc gatgataatc accgtccggt tgtgaaccag
    14221 gttatcgatc gctttttgca ccgccagctc gctttcaata tccagcgcgg cagtcggttc
    14281 atcgagaatg acgaccggcg cgtttttcaa taacgctctg gcaatggaaa tccgctggcg
    14341 ctcgccgccc gatagctggc cgcccatctc tcccattggc gtcagccagc cttgcggaag
    14401 acgggaaata aactcaaggc actgcgccgc gcgggcggcc tcttctacct cctgccgcgt
    14461 cgcctgtggg cgcgcgatac ggatattcgc cagcagcgtg tcatcaaaca gccagacatc
    14521 ctggaaaaca acagaaatca ggctattgag ctgttccggc gtcaggcggc gaatatcgac
    14581 gccgccaata gaaatctgcc cttgctgcgg atcggcatag cgcattaaca gtttggtgac
    14641 cgtagttttg cctgcgccgg aggcacccac cagcgcgctc atactggctg ccgggaacgt
    14701 caaagaaaca tgattaagcg cgtggccgtc accttcttcg tagcgatagc tgacgttgtc
    14761 aaagcggata tcgtaacgtt ccggcatctc gctttgctct gcgacgggag gcggtgctat
    14821 cgccataaac tgctcaatcc gttgcagggc gctggcgatc agttccacaa ccgaggtgta
    14881 gctgataaac atcgccatcg gttctgcgaa gcgcataatc atcgcgacgg cggcaatcaa
    14941 aaaggcgagg ttcagagtgc ccgtcactac ccagacgatc ccggataaca ccaccacctg
    15001 taggcccaac tccacgacgc tggcgatcag catcgttgcg ccagcgccct gacggtgagt
    15061 gcgggtctgt aagttttcca gcgcattgaa atgcgccagc agcgctcggc ttttatcggc
    15121 atcgctgccg caggtgcgca ataccatcat cccctgagca aattcaacga tatcgccgct
    15181 cagacgctgg tgcgcttccc ccagcgtctg catttgtcgc cgcatcgccg ggcggcgcca
    15241 gtaataaaac ggcaccagca gagggaagat cagcaacatc accagcccca gccgccagtc
    15301 tatccacaat gtcgccagcg acgccgtcag cggcgtgaca atggtgagca gcaaaatatt
    15361 ggctatcgca ataacataat tgaggttttc atccacgctg cccagcagca aggcgttcat
    15421 ttcacccgcc ctgccgcgct ggagcttctc cagcggcacg cggcgtaact gctcgccaag
    15481 tcgcaggcgc aactcatggg tagcctgcgc cagatgacca cggtattcaa agcccaggcc
    15541 ataccagcgt agcaccagcg tcacaattgc ggcgacgctg aaggccatag cccagttaag
    15601 aagttgcggc gcgtctcccc gtaacagcgc atcaatgatc ggataaagac aggcaaaggc
    15661 gatcccctgc atgaatgccg ccagcaacag cgccagcata ctgcgtcgca gcatcctggc
    15721 ctgactgcca acgctggaga taagctggcg ccagatgacg cgccaggcta ggttatccgc
    15781 aggattattg tctttcattt tccaccacct cttcctgtgt tccacccggc acccaatgct
    15841 gcgcctgctg atagtgttgc cagagccgtt gatacaggcc gccctgcgcc aacagttgcg
    15901 tatggttccc catttccctg agttgtccgt cggaaaacag caatatcaca tcggcctgag
    15961 tcaccattga gagacgatgc gcgaccatga tgaccgtccg gccacgcatg gccgccgcga
    16021 gcgccttgat aagcgccgct tcgttttccg ggtcagcaaa cgccgtcgcc tcatcgagca
    16081 ccaggatggg gcgatcctgc aaaagcgccc gggcgatagt aatgcgctgc cgctggccgc
    16141 cggagagaaa taccccacgt tccccgactc gagtgttgta accttctggc agagcgctaa
    16201 taaaatcatg ggcctgcgcc actctggccg ccgctataac cgcctccagc ggcgtatccg
    16261 gcgcgcccag acgaatgtta ttggctatcg tgtcggcaaa aaggaagttg tcctgaaaca
    16321 caaacgagag ttgcttcatc agggtgtccg tctgcatatc acgcagatcc acgccgccaa
    16381 tacggatatg gcctttgtcc gggtcggcgt aacgcagcag caagcgcgcc acggtgcttt
    16441 ttccggcgcc gcttggcccg accagcgcca caatttgccc ggcaggcaca tgaaagctca
    16501 cctcctgcaa cgcggcgcca gtacgcgctt gcggataatg aaagctcacc tgctcaaagg
    16561 taatgctcgc ctcttgcggc tgctggtcag actgcggcag cgataactcc ggcatcgcga
    16621 gaacctgata aatacgctga atgcttaaac gcgtttgcgc gaccaggtta ttgagcatca
    16681 tcattggcat tacggcttcg gccattccgc tgcccagcag taaaacggcc acccacgcga
    16741 taaaatcgaa actgccatag tgcaacaggc cgtatcccga ccagatcagg acaaacaggg
    16801 tcgggagagg attcagaatc gagaaggaaa aacgcgctga aaaaccggct ttgcgatacc
    16861 aggttttgag cacatcgacc cactcttcaa gggcgcgttg atagcgcaaa aaactggtgc
    16921 tgccgctatc aaacgttctc accacgggca tggcctgcac gaactcaata accgccgcgc
    16981 taacctgctc gcgcgcctta tgataacgct gagccatatt ttccgaacgc tggcgggcga
    17041 gcacgagaac aaccgatcca aacgccagta cccccagcgt cgcgattgcc agccgccagt
    17101 ccagccagaa caagataacg attgtcgcca gcggcatgat gatcgcgcgg gcatagagcg
    17161 gcgtgctgtc ggccacaaaa atatgtaacg acttcacgtc atccagcatc actttcgcca
    17221 ctgacccgct gcccatttgc tgtaacacgc caggcggaag ctgcaatgct ttacgggcta
    17281 acctgctgcg cagaatgttc tccagatgaa aagccgcgta atgagaggtg ttgaacgcct
    17341 gtaagcgcag aacaaaagtt aagaccacgc agccgatcac gccgcccagc ggccaggccg
    17401 gaatagcgtc aggcgtcgcg cgaatgtcac gcaggctcca cgccagaaaa aggagcgccc
    17461 ccaggctggt taatgccgcc agccccgcca gcgccatagc taaaataacc ttcctgcgaa
    17521 ccggtgtaat cacctgccag agaggaaatc gggacagcga ttcagtattg gatgattgag
    17581 atgacatggg agtaactgaa tttcctgatg aatttttacg gacgccaaaa acgacaattc
    17641 agggtggcgt tctgagaatt aatgtgaata ataaccatta tcaataactc atctacccca
    17701 ttcggttcta tttatacccg cattggtcta agccacaggg agataaccag gtcatgacgg
    17761 agtcaccgca aacgcaatct gaaatctcta ttcaccagtt ggtggtcggg aaacctgcaa
    17821 acgatggcaa tattcctgct cagtgtgaac tgctgcgctg ttcgttgcag gaaggtatgg
    17881 atattttgct ctggaggggc cattttgcgc ggccagagac gttgcagcta cacgacgatc
    17941 tgggcaggat taacttctcg tgcatcctgg aaggcacatc gcgctttgcg attcagggac
    18001 tccggcgcca caccgactgg gagcttgcgc gcaatcgcca ttacattacc catacgccag
    18061 actgtcgcgg cagcgcctcc tattgtggcc gattcgagag cattaccctc tccttcagcc
    18121 ctgaaaccct cgcgctgtgg gtgccggata ttagcgcggt cattaagaat aagatcgact
    18181 cccactgctg ttgccagcag cagcgttgta atgctgaaac ccacttaacg gctcaggcct
    18241 tgcgtcacgc gctgatgcgc atgcacggcg gcttcagtca tgaacaaaag ccgtcgacgc
    18301 tatggttgca ggggcaaagt ctggtcatgc tcagcctggt gctggatgaa catcgcgaag
    18361 acgccagttg cctctcctgc cacttcaacc cgatggaacg ccagaaactg ctgcgggcga
    18421 aggatctgct gctggctgat ctgacgcaag cgccgggcgt cgcggagctg gccagagagt
    18481 ccggtctgag cgttctcaaa attaagcgag gcttccgcgt tctgttcaac aacagcgtct
    18541 atggcctgtt tcaggctgaa cgaatgcagg aagccagaag acgcctggcc aacggcaata
    18601 cctccgtcat gacggtggcg gccgatctgg gctatgccaa cgccagccat ttctctgccg
    18661 ccttccagaa acagtttggc gtaactcctt cgacctttaa acgcgggatg taaccggggt
    18721 cgcgcccccc taaactcctc cctgacagag gcccggatac ctcatccggg cctctgttct
    18781 ttccgcctgt taccaacccg aaacgggcat aaataaaccc gtttcgggta gcatccaatt
    18841 gttaataatt attattctca tatgagtaat gcttttcggt aagacgtgcc atcaggagga
    18901 agaatgattt ctggcgcacc atctcaggat tcgctgttac cggacaaccg ccacgcggct
    18961 gattaccaac aattacgcga gcggctcata caggaactga atttaacgcc gcagcagtta
    19021 catgaagaga gcaacctgat ccaggccggc ctggattcca taagattgat gagatggtta
    19081 cactggtttc gtaaaaatgg ctaccgcctt acccttcgcg agctgtatgc cgcccccacg
    19141 ctggcggcat ggaaccagtt aatgctcagc cggtcgccgg agaacgcgga agaagaaacg
    19201 ccgcccgacg aatcatcctg gccgaacatg accgaaagta cccccttccc attgacgcca
    19261 gtacagcacg cctacctgac gggccgcatg ccggggcaga cgcttggcgg cgtgggttgc
    19321 cacctgtatc aggagtttga aggccattgt ctgacggcgt cgcagctgga gcaggccatc
    19381 acgaccttgc tgcaacgcca cccaatgctg catatcgcct ttcgccccga cgggcagcag
    19441 gtctggctac cgcaacctta ctggaacggc gtcaccgttc atgatttacg ccataacgac
    19501 gctgaaagcc gccaggccta tctggacgca ctgcgccagc gcctgagcca ccgtctttta
    19561 cgcgtggaaa tcggcgaaac gtttgatttt cagctgacgc tcttgccgga caatcgccac
    19621 cgcctccatg tcaatattga cctgctgatt atggatgcct ccagctttac gcttttcttc
    19681 gatgagctta acgccctgct ggccggagaa tcgctgccgg ctatcgacac ccgctatgat
    19741 ttccgctcgt atttgctgca ccagcagaag atcaatcaac cactgagaga cgacgcgcgc
    19801 gcttactggc tggcgaaagc atcgacgctt ccccccgcgc ccgtcttgcc gctggcctgc
    19861 gaacccgcca cgctacgtga agtccgtaat acccgacgcc gcatgattgt cccggcaaca
    19921 cgctggcacg cctttagcaa ccgggccggc gagtatggcg tgacgccgac aatggcactg
    19981 gcgacctgtt tttctgccgt gctggctcgc tggggcggcc tgacgcgtct gctgcttaac
    20041 atcaccttat tcgaccgcca gccgctgcac ccggcggttg gcgcgatgct tgccgacttc
    20101 accaatattc ttctgctgga taccgcctgc gatggcgata ccgtcagcaa cctggcgcgt
    20161 aaaaaccagc tcacgtttac ggaggactgg gagcatcgcc actggtccgg cgtcgaatta
    20221 ctccgtgaac tcaaacgcca gcagcgctac ccccatggcg ccccggtggt atttaccagc
    20281 aatctggggc gttccctcta cagcagccgc gcagaatcgc cgttgggcga gccggaatgg
    20341 ggcatctcgc aaacgccgca ggtctggata gatcatctgg cgttcgagca tcacggcgag
    20401 gtctggctac aatgggacag caacgacgcg ctgttccctc cggcgttagt cgaaacattg
    20461 ttcgacgcct actgccagtt gattaaccaa ctctgcgatg acgaaagcgc ctggcaaaag
    20521 ccgttcgcag atatgatgcc cgccagccag cgcgcgatac gcgaacgggt caacgccacc
    20581 ggcgccccca ttcccgaagg cttgctgcat gaaggcattt tccgtatcgc tctgcaacag
    20641 ccgcaggcgc tggcggtaac ggacatgcgt tatcagtgga attatcatga gctgacagac
    20701 tatgcccgcc gttgcgcggg caggttaatc gagtgcgggg ttcagcccgg cgataatgtg
    20761 gctatcacga tgtcgaaagg cgcaggacaa cttgttgcgg ttctggccgt cctgctggcc
    20821 ggggcggttt acgttccggt ttcgctggat cagcctgccg cacggcgcga gaaaatctac
    20881 gctgacgcca gcgtccggct ggtgctcatt tgtcagcacg acgccagcgc cgggtcagac
    20941 gatattcccg tccttgcctg gcagcaggcc attgaggcgg agccgatcgc caacccggta
    21001 gtacgcgccc ccacgcaacc ggcctacatt atctacacct ccggctctac cggtacgccg
    21061 aaaggggtag tcatttctca ccggggagcg cttaacacct gttgcgatat caatacccgc
    21121 tatcaggttg gcccgcatga cagggtgctg gccctctccg ccctacattt tgatttatcg
    21181 gtttacgaca tttttggcgt actgcgcgcg ggcggcgcgc tggtgatggt gatggaaaat
    21241 caacggcgcg atcctcacgc atggtgtgag ctgatccagc gccatcaggt cacgctctgg
    21301 aacagcgtcc cggcgctgtt cgatatgctg ctgacctggt gtgaaggttt cgccgacgcc
    21361 acgccggaaa acctgcgcgc agtgatgctt tccggcgact ggatcgggct tgacctcccc
    21421 gcccgttatc gggccttccg gccacaagga caatttatcg cgatgggcgg cgccaccgag
    21481 gcgtctatct ggtctaacgc ctgcgaaatt cacgacgtcc ccgcccactg gcgctccatc
    21541 ccttacggtt ttccgctaac caaccaacgc taccgggtgg tggatgaaca gggccgggac
    21601 tgccctgact gggtgccggg tgaattatgg attggcggca ttggggtcgc ggaaggctat
    21661 ttcaacgatc ccctgcgtag cgagcagcaa tttttgacgc tcccggacga gcgctggtat
    21721 cgcaccggcg atctcggctg ctactggcca gatggcacaa tcgagttcct cggtcgtcgc
    21781 gacaagcagg tcaaagtcgg aggatatcgc atcgagctgg gcgaaatcga aagcgcgctc
    21841 agccagctgg cgggggtgaa acaagcaacc gttctggcga tcggcgaaaa agaaaaaacg
    21901 ctggcggcat acgttgttcc tcagggcgag gctttttgcg ttaccgatca tcggaacccg
    21961 gcactgccgc aggcgtggca cacgcttgcg ggaacgttgc cctgttgcgc catctcgcca
    22021 gagatctccg cagaacaggt agccgatttc cttcagcatc gcctgctaaa actgaagccg
    22081 ggtcacaccg ctggcgccga tcctctcccc ctgatgaact cactcgctat ccagccgcgc
    22141 tggcaggccg tggtggaacg ctggttagca tttctggtga cacaacggcg actgaagccc
    22201 gctgctgaag gttatcaggt ctgcgctggt gaagaacgcg aggatgagca cccgcacttc
    22261 agcggacatg atttaacgtt atcgcaaatt cttcgcggtg cccgtaacga actgtcgtta
    22321 ctgaacgacg cgcagtggtc gccggaaagc ctggccttta accatccggc cagcgccccg
    22381 tatattcagg aactggcgac aatttgccaa cagcttgcac agcgcttaca gcgcccggta
    22441 cgcctgcttg aggtgggaac ccgcactggc cgcgccgcag aatcgctgtt agcacagctc
    22501 aacgccggac agattgagta tgtcgggctt gagcagagcc aggagatgct gctgagcgcc
    22561 cggcagaggc tcgccccctg gcctggcgcc cgtctgtccc tctggaatgc agacacgctg
    22621 gcgacgcacg ctcactcggc ggacattatc tggcttaata acgccctgca tcgtctgctg
    22681 ccggaagatc ccgggctcct tgcgacatta caacagcttg ccgttcccgg cgcgctgctc
    22741 tacgtgatgg agtttcgcca gttaacgccg tccgccctac tcagcacgct cctgttaacc
    22801 aatgggcagc cggaggcctt gctgcataac agcgccgact gggcggcatt atttagcgcg
    22861 gccggcttca actgtcagca tggcgatgag gtcgcggggt tacaacgctt cctcgtacaa
    22921 tgtcctgaca ggcaggtgcg ccgcgatccc cgtcaacttc aggccgccct cgccgggcgt
    22981 ctgccggggt ggatggtgcc gcaacggatc gtattcctcg acgccttacc gctgatggct
    23041 aacgggaaaa ttgactacca ggcgctgaag cgtcgtcata cccctgaagc ggaaaacccg
    23101 gccgaagcgg atttacccca gggcgacatt gaaaaacagg ttgccgccct ctggcagcaa
    23161 ctcttatcaa caggcaatgt caccagagaa accgacttct tccagcaagg cggcgatagc
    23221 ctgctggcga cccgtctgac cgggcaactt catcaggcag gttatgaagc gcaattaagc
    23281 gacctgttta atcatccccg gctggcggat tttgccgcca cgctgcggaa aaccgacgtc
    23341 ccggtcgaac aaccattcgt ccactcccct gaagatcgct accagccctt tgcgcttacc
    23401 gacgtgcagc aggcttacct ggtggggcgt cagccgggct ttgccctggg cggcgtcggc
    23461 tcacatttct ttgttgaatt tgaaattgcc gatctggacc tcacccggct ggagacggtc
    23521 tggaaccgat taatcgcccg ccacgatatg ctgcgcgcca tcgtgcgtga tggacagcaa
    23581 caggtgctcg aacagacgcc cccttgggtg atacccgcac acaccctcca tacgcctgaa
    23641 gaggcgttgc gggtgcgcga aaaactggcg catcaggtac tcaaccccga agtgtggccg
    23701 gtattcgatc tccaggtcgg atacgcggac gggatgcctg cccgcctgtg gctgtgtctg
    23761 gataacctgt tgcttgacgg tctgagcatg cagatcctgc tggcggagct ggagcacggc
    23821 taccgctacc cgcaacagct gcttccgccg ctgcccgtca ccttcaggga ttatctgcaa
    23881 caaccctcgc tacagtcgcc caatccagat tctctggcat ggtggcaggc gcagcttgat
    23941 gatattcctc cggcgcctgc gttgccgctg cgctgcttgc ctcaggaggt tgaaacaccg
    24001 cgcttcgccc gcctgaacgg cgcactggac agcacgcgct ggcatcggct gaaaaaacgg
    24061 gcggctgacg cccatctcac cccgtcggcc gtactgttgt cggtgtggtc aacggttctc
    24121 tctgcatgga gtgcacagcc tgagttcacg cttaacctta cgcttttcga caggcgaccg
    24181 ctgcacccgc aaatcaacca gattctgggc gatttcacct cgctgatgct gctgagctgg
    24241 catcccggcg aaagctggct gcacagcgcg cagtcactac agcagcggct gagccagaac
    24301 ctcaaccacc gcgatgtgtc agccatccgc gtgatgcgtc aactggcgca acggcaaaac
    24361 gtgcctgccg ttccgatgcc cgtcgtcttt accagcgcac tgggctttga gcaggataac
    24421 ttcctcgccc ggcgtaatct gctcaaaccg gtctggggca tctcccagac gccgcaggtc
    24481 tggctcgatc accagattta tgaatccgaa ggcgaactgc gctttaactg ggattttgtc
    24541 gccgcgctgt ttcctgccgg gcaggtggag cgccagtttg aacagtattg cgcattgcta
    24601 aaccgaatgg ccgaggatga aagcggctgg caactgccgc tcgccgcgct ggtgcctccc
    24661 gttaaacacg cagggcaatg cgcagagcgc tcaccgcgcg tatgccctga gcactctcag
    24721 ccacacattg cggcggacga gagcaccgtc agcctgattt gcgacgcctt ccgcgaggtg
    24781 gttggcgagt ctgtcacgcc cgcagaaaac ttctttgagg cgggcgcaac gtcgctgaat
    24841 ctggtgcaac tgcacgtttt gttacaacgt cacgaatttt ccaccctgac gttgcttgac
    24901 ctcttcaccc acccttctcc tgctgccctg gccgattatc tggccggcgt cgccacggtg
    24961 gagaaaacaa aacgacctcg ccctgttcgc cgtcgtcagc ggcggatata gcgcgaagca
    25021 aactgatttt ccccggaacg ccatcgcgaa cgcatggcgt tccattgact ttgtgaatct
    25081 taggaaacgg gaccgattat ggataacttg cgcttctctt ctgcgccgac agcagattcc
    25141 attgatgcat cgatcgctca acactacccg gactgcgaac ctgtcgcggt tatcggctac
    25201 gcctgccatt ttcctgaatc gccggatggc gaaacgttct ggcaaaatct gctggaaggt
    25261 cgtgaatgca gccgacgctt tacgcgcgaa gagcttctgg ccgtcggtct ggatgccgcc
    25321 atcattgacg atcctcatta tgtcaatatc ggtacggtgt tagacaacgc cgactgcttc
    25381 gacgccaccc tgtttggcta ttcgcgacag gaagcggagt cgatggaccc gcagcagcgc
    25441 ctgtttttgc aggcggtctg gcatgcgctg gaacatgccg gttatgcccc cggcgccgtc
    25501 ccccataaga ccggcgtttt cgcctcttcc cggatgagta cctaccccgg tcgcgaagca
    25561 ttgaacgtga cagaagtcgc gcaggtaaaa ggtctgcaat ctctgatggg caatgataaa
    25621 gactatattg ccacccgcgc cgcgtacaaa ctcaacctgc acggcccggc gttatcggta
    25681 cagaccgcct gctccagctc gctggttgcc gtgcatctgg cctgtgaaag cctgcgcgca
    25741 ggcgaatccg atatggcggt ggccggcggc gtggcgctct ctttccccca gcaggcaggc
    25801 taccgctacc agcccggaat gattttctct cctgatggtc actgtcgtcc ctttgacgcc
    25861 tcggctgagg gcacctgggc cggtaacggt ctcggctgcg tggtgctgcg tcgcctgaga
    25921 gacgcgctgc tgtcaggcga tccgattatc tcggtgatcc tctccagcgc ggtcaacaac
    25981 gacggcaaca gaaaggtcgg ctataccgcc ccttccgtcg cagggcaaca ggcagtcatc
    26041 gaagaggcgt taatgctggc ggccatcgac gacaggcagg taggttacat tgaaacccac
    26101 ggcaccggca caccgctggg cgacgcgatt gaaattgaag cgttacgcaa cgtctatgcg
    26161 cctcgcccgc aggatcagcg ctgtgcgctc ggttccgtga aaagtaacat gggccatctg
    26221 gataccgcgg cgggcattgc cggactgctg aaaaccgttc tggcagtcag tcgcgggcaa
    26281 attcctccct tactgaattt tcacaccccc aacccggcgc tgaaacttga agagagcccc
    26341 tttaccatac cggtgtcggc acaggcatgg caggacgaaa tgcgctatgc gggcgtctcc
    26401 tcctttggta ttggcggcac caactgccat atgatcgtcg cctcgctgcc cgacgcgctc
    26461 aacgcgcgcc tccccaatac ggatagcggc agaaaaagta ccgcgctgct gctcagcgcc
    26521 gccagcgaca gcgcgttgcg gcggctggcg acggattatg ccggggcgct gagagagaat
    26581 gcggatgcca gctctctggc cttcacagcc ctgcacgcgc gccgtctcga tctccccttc
    26641 cgcctggcgg cgccattaaa ccgtgaaacc gccgaggcgc tcagcgcctg ggccggtgag
    26701 aaatcggggg cgctggttta cagcggccac ggcgccagcg gcaagcaggt gtggctgttt
    26761 accggccagg gctcgcactg gcgcactatg ggtcaaacga tgtaccatca ctcaacggcg
    26821 tttgccgaca cgctggatcg ctgtttttcc gcctgtagcg aaatgctcac gccgtcactg
    26881 cgcgaagcga tgtttaaccc cgattcggcg cagctggaca atatggcctg ggcgcagccg
    26941 gcgattgtcg cgtttgaaat cgcgatggcg gcgcactggc gtgctgaagg actgaagcca
    27001 gacttcgcca ttgggcattc cgtcggtgaa tttgccgctg ccgttgtctg cggacactat
    27061 acgattgaac aggtcatgcc actggtttgt cggcgcggcg cgctaatgca gcagtgcgca
    27121 agcggcgcga tggtggcggt atttgcagac gaagacacgc tgatgccgct ggctcgccag
    27181 tttgagctgg atctcgccgc caacaacggt acgcaacata cggtattttc cgggccggaa
    27241 gcccgtctcg cggtattttg cgccacgctc tcgcagcatg acattaacta tcgtcgcctg
    27301 agcgtaaccg gtgcggcgca ctccgcttta ctggagccga tactcgatcg gttccaggac
    27361 gcctgcgcgg gactgcacgc ggagccgggg caaataccga ttatttccac gctcaccgcc
    27421 gacgtcattg atgagtcaac gctcaaccag gcggattact ggcgccgaca catgcgccag
    27481 ccggtgcgtt ttatccagag tattcaggtg gcgcatcagc tcggcgcccg cgtttttctg
    27541 gagatggggc ccgatgccca gttggttgct tgcgggcagc gcgaataccg cgataacgca
    27601 tactggatag ccagcgcccg gcgtaacaaa gaggcgagcg atgtcctcaa tcaggccctg
    27661 ctccagcttt acgctgccgg cgtcgcccta ccgtgggccg acctgctggc gggcgatgga
    27721 caacgtatcg ctgcgccatg ttatccgttt gatactgaac gttactggaa agagcgcgtc
    27781 tccccggcct gcgagcctgc cgacgcagcg ctgtctgccg ggctggaggt ggcgagtcgc
    27841 gccgcgacag cgctcgatct ccctcgcctg gaagcgctta aacagtgcgc cacgcgactg
    27901 cacgccatct acgtcgatca actggtacaa cgctgtaccg gcgatgccat tgagaacggc
    27961 gtggacgcca tgaccatcat gcgccgtgga cgtctgctgc cccgctacca gcagctactc
    28021 cagcgcctgc tgaataactg cgtggtcgac ggcgattacc gctgcaccga cgggcgatac
    28081 gtccgcgccc gccccattga acatcaacag cgggaatcac tgctgacgga acttgccggt
    28141 tattgtgaag gttttcaggc tattcccgac accatcgccc gtgccggcga tcggttatat
    28201 gaaatgatga gcggcgcgga agaaccggtg gcgattatct tcccgcaaag cgcctccgac
    28261 ggcgtggaag tgctgtatca ggaattcagc tttggccgct atttcaacca aatcgccgcc
    28321 ggggtattac gcggcattgt ccagacgcgt cagccccgcc agccgttgcg tattcttgaa
    28381 gttggcggcg gaaccggcgg caccaccgcg tggctgctgc cggaactcaa cggcgttccg
    28441 gcactggagt accatttcac cgatatctcg gcgctgttca cccgtcgcgc ccagcagaaa
    28501 ttcgccgact acgattttgt gaagtatagc gagctggatc tcgaaaaaga ggcgcagtct
    28561 cagggtttcc aggcacagtc ttacgatctt atcgtggcag cgaacgtgat tcacgccacc
    28621 cgccatattg gccgcacgct cgataatctg cgccccctgc tcaagccagg cgggcgcctg
    28681 ctgatgcgcg aaatcaccca gccaatgcgt ctgtttgact tcgttttcgg cccgctggtt
    28741 cttccgctac aggatctcga cgcccgcgaa ggtgagttat tcctcaccac cgctcagtgg
    28801 caacaacagt gccgccacgc cggattcagc aaagtggcgt ggctaccgca ggatggcagc
    28861 ccgaacgccg ggatgagcga acatatcatt ctcgccacgc tgcccggtca ggcggttagc
    28921 gccgtaacat tcaccgcgcc atcagaaccc gtgttggggc aggcgctgac ggataacggt
    28981 gattatctcg ccgactggtc tgattgcgca ggtcagcccg aacagtttaa cgcccgctgg
    29041 caggaggcat ggcgtctgct ttcacagcgt catggcgacg ctctgcctgt ggaaccgccc
    29101 cccgtcgccg ccccggagtg gctggggaag gttcgcttaa gctggcaaaa cgaagccttt
    29161 tcccgcggtc agatgcgcgt tgaagcccgt catcctgctg gcgagtggct gccgctatcg
    29221 cccgccgcgc ctcttcctgc gccgcaaacg cattatcaat ggcgctggac gcccctcaac
    29281 gtcgccagca ttgaccatcc gcttaccttt agcttcagcg ccggtacgct tgcgcgcagc
    29341 gacgagctgg cgcaatacgg catcattcac gatccgcacg cctcttcacg actgatgatt
    29401 gttgaggaga gcgaggatac gctggcctta gcggagaaag tgatagcagc gctcaccgcc
    29461 agcgcagccg gattgattgt ggttactcgc cgcgcgtggc gagtcgagga aaatgaagca
    29521 ctctctgcat cccatcacgc gctatgggcc ttgcttcgcg tcgcggccaa cgaacagccg
    29581 gaacggttgc ttgccgccat cgatctcgcc gaaaacaccc cgtgggaaac gctgcatcaa
    29641 gggttgagcg cagtctcact accacagcgc tggctcgccg cacggggtga caccctttgg
    29701 cttccttcac tgtcgcccaa tacgggatgc gccgctgaat taccggcaaa cgtgtttacc
    29761 ggcgatagcc gctggcatct ggtgaccgga gcgtttggcg gattaggccg ccttgccgtg
    29821 aactggctca gagaaaaagg ggcgcgacgc atcgccctgc tggcgccgcg cgtggatgag
    29881 tcatggctac gcgacgtgga gggcgggcag acgcgcgtct gccgttgtga tgtgggcgat
    29941 gccgggcaac tggccacggt tcttgacgat ctggcggcca acggcggcat tgccggagcg
    30001 attcatgccg ctggcgtatt ggctgacgcg cccttgcagg agcttgatga ccaccagctg
    30061 gctgccgttt tcgcggtaaa agcgcaggcg gcaagccagc tgttgcaaac cctgcgcaac
    30121 cacgacggac gctatcttat tctctactct tccgctgccg ccaccctcgg cgcgccgggt
    30181 cagagcgccc atgcgctggc ctgcggctac ctggacgggc tggcccagca gttttccacc
    30241 cttgatgcgc cgaaaacgct ctctgtcgcc tggggcgcat ggggagaaag cggtcgggcg
    30301 gccacgccgg aaatgctggc gacgctcgcc agccgaggta tgggcgcgtt aagcgatgcc
    30361 gaaggctgct ggcacctgga acaggcggtg atgcgcggcg ccccgtggcg actggcgatg
    30421 cgcgttttta ccgacaaaat gcccccgtta caacaggctc tgtttaacat cagcgccaca
    30481 gaaaaagccg caacgccggt cattcctcct gctgatgaca acgcctttaa cggcagcctg
    30541 agcgatgaaa cagcggtgat ggcatggctg aaaaagcgga ttgcggttca gctaaggctg
    30601 agcgatccgg cgtcactgca tccaaaccag gatctgttgc aactcggcat ggactcgctg
    30661 ctcttccttg aactcagtag cgatattcag cactacctgg gtgtacgcat caatgcggaa
    30721 cgggcgtggc aggatctgtc tcctcatgga ctcacgcagc ttatctgttc taagccagag
    30781 gcgacgcctg ccgcttcgca gccggaagtg ttgcggcacg acgccgacga gcgttatgcg
    30841 cccttccctt tgacgcccat tcagcacgcc tactggctgg ggcgaaccca cttcattggc
    30901 tatggcggcg tcgcctgtca cgtcctgttt gagtgggata aacgccacga tgagttcgat
    30961 ctcgccatac tggagaaagc atggaaccag ctcatcgcac gccacgatat gttgcgtatg
    31021 gtggttgatg ccgacgggca gcagcgaatc ctggcgacaa cgccggagta tcacatcccg
    31081 cgtgacgatc tgcgcgcgct ttccccggaa gaacagcgca tcgcgctgga aaaacggcgg
    31141 catgaactga gctatcgcgt tttgcctgcc gaccagtggc ctctttttga gctggtggtc
    31201 agcgaaatcg acgattgcca ttaccgtctg catatgaacc tcgacctttt gcagtttgat
    31261 gtgcagagtt ttaaagtcat gatggacgac ctggcgcagg tctggcgcgg tgaaacgctg
    31321 gcaccgctcg ctattacctt ccgtgattat gtgatggctg aacaggcgcg ccgacagaca
    31381 tcggcatggc acgatgcctg ggattactgg caggaaaaac tgccgcaact gcccttagcg
    31441 ccagagctgc cggtggttga gacgcccccg gaaacgccac acttcaccac cttcaaatcg
    31501 acgatcggca agacagaatg gcaggccgtg aaacagcgct ggcagcagca aggcgtcaca
    31561 ccgtctgccg cgctgctcac gctgtttgcc gccacccttg agcgctggag ccgtaccaca
    31621 acatttacgc tgaacctgac gttcttcaat cgccagccga tccatccgca aatcaaccag
    31681 ttgattggtg attttacctc cgtcacgctg gttgatttta acttctcagc gccggtgacg
    31741 ttgcaagagc agatgcaaca gacccaacag cgcctctggc aaaacatggc gcacagtgaa
    31801 atgaacggtg ttgaggtgat ccgtgagctg ggccgcctgc gcggatcaca acgtcaaccg
    31861 ctgatgccga tagtgtttac cagtatgctg gggatgacgc tggaaggcat gactatcgat
    31921 caggcgatga gccatctgtt cggcgaaccc tgctatgtat tcacgcaaac gccgcaggtc
    31981 tggctggatc atcaggtcat ggagagcgac ggcgagttga tgtttagctg gtactgcatg
    32041 gacaacgtgc tggaacccgg cgctgccgag gcgatgttta atgactattg cgccatcctg
    32101 caagccgtca tcgccgcccc tgaaagcctg aagactctcg ccagcggcat cgccaggcac
    32161 attccccgcc gacgctggcc gctgaacgcg caggcggact acgacctgcg ggatattgag
    32221 caggcgacgc tcgaataccc cggcatccgg caggccagag cggaaataac cgaacagggc
    32281 gcgttgacgc tggatatcgt gatggccgac gatccttcgc catcagcggc gatgcctgat
    32341 gagcacgaac ttacccaact ggcgctgccg ttgcctgagc aggcgcagct tgatgagctg
    32401 gaggcgacct ggcgctggct ggaggcgcgt gcgctacagg ggatcgcggc tacgctaaat
    32461 cgtcacggcc tgtttaccac gccggagatc gcccatcgct ttagcgcaat agtacaggcg
    32521 ctgtccgcgc aagcgtctca ccagcgtctg ctgcgccagt ggctacagtg tctgacggaa
    32581 agagagtggt taatccgcga aggtgaaagc tggcgctgcc gcattccgct cagcgagatt
    32641 cctgagcctc aggaagcgtg cccgcaaaac caatggagcc gggcgctggc gcagtatctg
    32701 gaaacctgca tcgcccggca cgacgccctt ttctccgggc agtgttctcc gctggaattg
    32761 ctgttcaacg agcagcatcg cgttaccgac gcgctgtatc gcgacaaccc cgccagcgcc
    32821 tgtctgaatc gctataccgc gcagattgcc gccttgtgca gcgcagaacg gattctggag
    32881 gttggcgccg gaaccgcagc cactaccgcg ccggtgctga aggccacgcg gaacacgcga
    32941 cagtcgtacc acttcacgga cgtctccgcg cagttcctca atgacgccag agcccgtttc
    33001 catgatgaat cgcaggtgtc ttatgccttg ttcgacatca accagccgct ggatttcacc
    33061 gcccacccgg aggcgggtta cgacctgatc gttgccgtca atgtgctcca cgacgccagc
    33121 catgtcgtcc agacgttgcg cagattaaaa ctgttgctga aagccggcgg acgtttgctg
    33181 atcgttgaag cgacggagcg aaacagcgta ttccagctgg cgagcgtggg ctttattgag
    33241 ggattaagcg gataccgcga tttccgccgc cgggatgaga aaccgatgct cacccgctcc
    33301 gcatggcagg aggttctcgt tcaggccggg tttgcaaacg agctggcgtg gcccgcgcag
    33361 gaatcgtcgc cgctgcgcca gcatctgctg gtagcgcgtt cgcctggcgt aaatcgcccg
    33421 gataaaaaag ccgtgagccg ctatttacag cagcgctttg gcaccggtct gcccatttta
    33481 cagatccggc aaagagaagc gttatttacg ccgctgcatg ccccgtctga tgcgccgact
    33541 gagccagcca aacccacgcc agttgccggg gggaatccgg cgctggaaaa acaggtggct
    33601 gaactctggc aatcgctgct gtctcgcccc gtggcaaggc atcacgactt tttcgaactg
    33661 ggcggcgaca gcctgatggc gacaaggatg gtcgcgcagc taaaccggag agggattgcc
    33721 agggctaacc ttcaggatct gttcagccat tcgacgctga gcgacttctg cgcccatcta
    33781 caggcggcta cgtcaggaga ggacaacccg ataccccttt gccagggcga cggtgaggaa
    33841 accctgtttg tcttccacgc ttcagacggc gatatcagcg cctggctgcc gctcgctagc
    33901 gcgttgaaca ggcgcgtttt cggcctgcaa gcaaaatcgc cgcagcgctt tgccacgctc
    33961 gaccagatga tcgatgagta tgtcgggtgc atccgtcgtc agcagcctca cggcccttat
    34021 gtgctggcgg gttggtcgta tggcgcgttt cttgcggcgg gcgccgcaca gcgcctgtac
    34081 gccaaaggca agcaggttcg gatggtgtta atcgatcccg tgtgccgaca ggatttctgt
    34141 tgcgaaaacc gggcggccct gctgcgcctg ttagccgaag gacaaacgcc tctggcactg
    34201 cccgaacatt tcgaccagca gacgcccgac agccagcttg ccgactttat cagcctcgct
    34261 aaaacggccg gtatggtgtc gcaaaacctg acgctgcaag cggcagaaac gtggctcgac
    34321 aacatcgcgc atctgctgcg tttactgact gagcatacgc cgggcgaaag cgttccggtc
    34381 ccctgtctca tggtgtatgc cgccgggaga cccgagcgct ggacgccagc agaaaccgag
    34441 tggcagggcc ggataaacaa cgccgacgac gctgtgattg aagccagcca ctggcaaatc
    34501 atgatggaag ccccccacgt tcaggcttgt gcgcaacaca ttacgcgctg gctttgcgca
    34561 acctcaacgc aaccggagaa cacgttatga tgccgtccgc ctccccaaaa caacgtgtac
    34621 tgattgtggg cgccaaattt ggcgaaatgt acctgaatgc ctttatgcag cccccggagg
    34681 ggctggaact ggtcggcctg ctggcgcagg gaagcgcccg ttcaagagag ctggctcatg
    34741 cgtttggcat tccgctgtat acctcgccgg aacagataac caggatgccg gatatcgcct
    34801 gtattgtcgt gcgttcgacc gtcgccggag gaaccggtac gcagcttgcc agacactttc
    34861 tgacgcgcgg cgtacacgtc attcaggagc atcccctcca cccggatgac atcagttcct
    34921 tacaaacgct ggcgcaggaa cagggctgct gctattgggt aaacacgttt tatccgcata
    34981 cgcgcgcggg acgcacatgg cttcgcgatg cgcagcagct gcgtcgctgc ctggctaaga
    35041 cgccgccggt agtccacgcc accaccagcc gacagttgct ctactccact ctggatttgt
    35101 tgttgctggc tctgggcgtt gatgccgccg ccgtggagtg cgacgtggtt ggcagcttta
    35161 gcgattttca ctgtctgaga ctcttctggc cggagggaga agcctgcctg ctgcttcagc
    35221 gctatctcga tcctgacgat ccagatatgc atagccttat catgcaccgc ctgctgctgg
    35281 gctggccgga aggtcatctt tcgctggagg cgagctacgg ccccgtcatc tggtcatcca
    35341 gcctgtttgt cgcagaccat caggagaacg cccacagcct ctggcgcaga ccggagatcc
    35401 tgcgcgatct gccgggtctg acgcgcagcg cggcgcccct gagctggcgc gactgctgcg
    35461 aaaccgtcgg gccggagggc gtcagctggt tgctacacca gttgcgcagc catctggcgg
    35521 gcgaacatcc accagcggcc tgccaaagcg tacatcagat cgcgttatca cgtttatggc
    35581 agcagatttt acgtaagacc ggcaatgcgg agatcagacg cctgacgccg ccgcaccacg
    35641 accggttagc cggtttctac aacgatgatg ataaggaggc gctgtgacgc aatctgcaat
    35701 gtgcataccg ctgtggcccg cccggaacgg caatactgcg catctggtca tgtgcccttt
    35761 cgctggcggc agcagtagcg cgtttcgcca ctggcaagct gagcaactgg ctgattgcgc
    35821 gctttctctg gtgacctggc cggggcgcga tcgccttcgc catctggaac cgctcagaag
    35881 cattacacaa ctggcggcac tgctggcgaa cgagctggaa gcatccgtat cgcctgacac
    35941 gccgctttta ctcgccgggc acagcatggg ggcgcaggtg gcgtttgaaa cctgccgact
    36001 tctggagcaa cgggggcttg cgccacaagg actgattatt tccgggtgcc atgccccgca
    36061 tctgcattct gaacgccagc tcagccatcg cgatgatgcc gactttatcg ctgagctgat
    36121 agacattggc ggatgttctc ctgaactgcg ggaaaaccag gaattaatgt cgctgtttct
    36181 tcctcttctg cgcgctgatt tttacgccac cgagagctat cactacgact cgcccgacgt
    36241 ctgtccgccg ctgcgcacgc ctgcgctgtt attgtgcggc agccacgatc acgaagcctc
    36301 ctggcagcag gtcgatgcct ggcgtcagtg gctgagccac gttacaggcc cggtggtgat
    36361 tgacggcgat cacttctatc ccattcaaca agcccggtcc ttttttacgc agattgtccg
    36421 ccattttccc cacgcatttt ctgcaatgac cgcgtggcaa aaacagccca gtacttcaga
    36481 aaggtgacgc atgaattctt cctttgaatc tctgattgaa cagtatccct tacccattgc
    36541 cgaacagttg cgccactggg cggcccgtta tgcctcgcga attgccgtcg ttgatgcaaa
    36601 ggggtcgtta acctacagcg cgcttgatgc acaagttgac gaacttgccg caggtctgtc
    36661 atcactgggt ttgcgttcgg gggagcatgt aattgtgcag cttcccaacg acaacgcgtt
    36721 tgttaccctg ctgttcgcct tgttaagact gggcgttatc cccgtgctgg cgatgccctc
    36781 gcaacgggcg ctggatatcg acgcgctgat tgagctggcg aaacccgtcg cttacgttat
    36841 tcacggggaa aaccacgcag agctggcccg acagatggcg cacaaacacg cctgcttgcg
    36901 tcatgttctg gtcgctggag agaccatgag cgacgatttt acgccgctct tctcccttca
    36961 cggtgagcga caggcatggc cgcagcctga tgtttccacc accgcgttgt tgttgctctc
    37021 aggcggcaca accagcacgc ccaaactcat cccgcgccga catgccgact atagctataa
    37081 cttcagcgct tctgctgaac tgtgcggcat cagccaacag agcgtgtatc tcgccgtcct
    37141 cccggtggcg cataactttc cgctggcctg ccccggtatt ctgggaaccc ttgcctgcgg
    37201 cggaaaagtg gtgctgaccg acagcgccag ctgtgatgag gtgatgcctt taatcgcgca
    37261 ggaaagagtg actcacgtcg ccctggttcc ggcgctggcg caattatggg tgcaggccag
    37321 ggagtgggaa gacagcgacc tttcgtcgct acgcgtcatt caggcaggcg gcgcccggct
    37381 cgacccgacg cttgctgagc aggttatcgc cacctttgac tgtaccctgc aacaggtttt
    37441 cggtatggcg gaaggcctgc tctgttttac ccggctggac gatccgcatg ccaccattct
    37501 ccacagccag gggcgcccgt tgtcccctct ggatgaaatc cgcatcgttg atcaagacga
    37561 gaacgacgtc gcgccgggcg aaaccgggca attgttaacg cgcggccctt ataccatttc
    37621 gggctattac cgcgcccctg cccacaacac gcaggccttt accgcgcaag ggttttaccg
    37681 cacaggcgac aatgtcaggc tggatgaggt ggggaacctg cacgttgagg gacgcataaa
    37741 agagcagatc aaccgcgccg gagaaaaaat agccgcggct gaagtggaat cggcactgct
    37801 gcgtttagcg gaagtgcaag attgcgcggt ggtcgccgcg ccggacacgc tgcttggcga
    37861 gcggatttgc gcgtttatca tcgcgcagca ggtgccaact gactatcagc agttgcgtca
    37921 acaactgacc cgtatggggc tcagcgcgtg gaaaattcct gaccaaatcg agtttctgga
    37981 ccactggccg ctcaccgccg tcggcaagat agacaaaaaa cgcctgacgg ctctcgccgt
    38041 cgaccgttat cgccattctg cccaataagc gcaaaccgac ccgaaacagg ttgaaataaa
    38101 cccgtttcgg gtagcaccac cattagaaat agttatcatt ttcaattcac cattgtcggt
    38161 attttttggc gtttcgccgt cttacaggga ctcacaacaa tgaaaatgac acggctttat
    38221 cctctggcct tggggggatt attgctcccc gccattgcta atgcccagac ttcacagcaa
    38281 gacgaaagca cgctggtggt taccgccagt aaacaatctt cccgctcggc atcagccaac
    38341 aacgtctcgt ctactgttgt cagcgcgccg gaattaagcg acgccggcgt caccgccagc
    38401 gacaaactcc ccagagtctt gcccgggctc aatattgaaa atagcggcaa catgcttttt
    38461 tcgacgatct cgctacgcgg cgtctcttca gcgcaggact tctataaccc cgccgtcacc
    38521 ctgtatgtcg atggcgtccc tcagctttcc accaacacca tccaggcgct taccgatgtg
    38581 caaagcgtgg agttgctgcg aggcccacag ggaacgttat atggcaaaag cgctcagggc
    38641 gggatcatca acatcgtcac ccagcagccg gacagcacgc cgcgcggcta tattgaaggc
    38701 ggcgtcagta gccgcgacag ttatcgaagt aagttcaacc tgagcggccc cattcaggat
    38761 ggcctgctgt acggcagcgt caccctgtta cgccaggttg atgacggcga catgattaac
    38821 cccgcgacgg gaagcgatga cttaggcggc acccgcgcca gcatagggaa tgtgaaactg
    38881 cgtctggcgc cggacgatca gccctgggaa atgggctttg ccgcctcacg cgaatgtacc
    38941 cgcgccaccc aggacgccta tgtgggatgg aatgatatta agggccgtaa gctgtcgatc
    39001 agcgatggtt caccagaccc gtacatgcgg cgctgcactg acagccagac cctgagtggg
    39061 aaatacacca ccgatgactg ggttttcaac ctgatcagcg cctggcagca gcagcattat
    39121 tcgcgcacct tcccttccgg ttcgttaatc gtcaatatgc ctcagcgctg gaatcaggat
    39181 gtgcaggagc tgcgcgctgc aaccctgggc gatgcgcgta ccgttgatat ggtgtttggg
    39241 ctgtaccggc agaacacccg cgagaagtta aattcagcct acgacatgcc gacaatgcct
    39301 tatttaagca gtaccggcta taccaccgct gaaacgctgg ccgcatacag tgacctgacc
    39361 tggcatttaa ccgatcgttt tgatatcggc ggcggcgtgc gcttctcgca tgataaatcc
    39421 agtacacaat atcacggcag catgctcggc aacccgtttg gcgaccaggg taagagcaat
    39481 gacgatcagg tgctcgggca gctatccgca ggctatatgc tgaccgatga ctggagagtg
    39541 tatacccgtg tagcccaggg atataaacct tccgggtaca acatcgtgcc tactgcgggt
    39601 cttgatgcca aaccgttcgt cgccgagaaa tccatcaact atgaacttgg cacccgctac
    39661 gaaaccgctg acgtcacgct gcaagccgcg acgttttata cccacaccaa agacatgcag
    39721 ctttactctg gcccggtcgg gatgcagaca ttaagcaatg cgggtaaagc cgacgccacc
    39781 ggcgttgagc ttgaagcgaa gtggcggttt gcgccaggct ggtcatggga tatcaatggc
    39841 aacgtgatcc gttccgaatt caccaatgac agtgagttgt atcacggtaa ccgggtgccg
    39901 ttcgtaccac gttatggcgc gggaagcagc gtgaacggcg tgattgatac gcgctatggc
    39961 gcactgatgc cccgactggc ggttaatctg gtcgggccgc attatttcga tggcgacaac
    40021 cagttgcggc aaggcaccta tgccaccctg gacagcagcc tgggctggca ggcgactgaa
    40081 cggatgaaca tttccgtcta tgtcgataac ctgttcgacc gtcgttaccg tacctatggc
    40141 tacatgaacg gcagcagcgc cgtcgcgcag gtcaatatgg gtcgcaccgt cggtatcaat
    40201 acgcgaattg atttcttctg attattgtaa aagggatacc gaaaaggtat cccttttaca
    40261 ccactagtta aaaccagtaa ctcagcagag ttgcaaaaaa tattaatcca tagtgattat
    40321 ttaaacaatg aaattgcgat taggataaat agatttaact ttctcgttcc tttctctcct
    40381 tatactaaag aaataatcat atcaaaataa aaattcacaa cagtgcaaca ttaaaaaata
    40441 caaccaacaa acaatcctat atacaaggca catctccaga atataaaagc acagacaaac
    40501 aacctaaaaa aacaacccca tacatataaa ttattttata ggtaaaatag attatatatt
    40561 ctcatagcta ccacaaacta tactagcctg aactatattt attctgctgc aatcaatgca
    40621 tacataacac aaatatcact caggtacact actcaaacca cgctgggatt tttcctcaag
    40681 ttataatcat caccccgaaa atcattcggc atttactcat taaatagtca ccccataggc
    40741 ctgtacatgt ttactcagaa atatacatcc ttttctctgt cataaaccct ctgattaatc
    40801 ataaataaat acttgtgaca ccaatctttt tccttaacgg aacgaattgt tgtgtagaag
    40861 gagataatat tatgtccaaa aaatatcagc cattgcttat aactcattat atgtcaacat
    40921 gggtcactat aacggaagca gttgagatca ctaccaaagc cataaaacaa aaaattactc
    40981 ctagcgatat ttatcgtcat gccttgagtg gcaacatcct actatcggtc tatttccagt
    41041 ctcctgtgat acttaagaag atacaaactt ttaatggaaa aataaaattc aggcagtttg
    41101 tgggagacct acttgataaa ctatgcatgc ttgacaggga cggttttatt tatggtcaaa
    41161 atttaagact ctgtacagaa gccagatata tttgtcctgt gcaacaaatt atagataccc
    41221 ccctgttacg caaactcaac cagtttcgta cgtttgttcg taatgtccgc ccgggtgatg
    41281 aactggatgt cccggcacaa gttagtgaaa aaaatttaac cccgccgccg ggtaacagca
    41341 gtggcaacct tgagcaacag atagccagta cttcacagct aattggatct ctgctcgccg
    41401 aagatatgaa cagcgagcaa gcggcaaaca tagcacgtgg atgggcctct tctcaggctt
    41461 caggggtaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg ctgggcgtgg
    41521 atgaagattt cagcctgaag aactcccgtg acggcaaccc tcgaaaatgg cgattcgatg
    41581 caacaaacag tgaactatgt gccgaacgtc acgaatgctg aaatcatgct ggcagcctcg
    41641 aaggatccgg tgattgccga caataacgat ctcacgacac taacagcacc gtcgctgata
    41701 cagagggcaa tgcgatagcc aacactgagg taacatttac tctaccggaa gatgtgaagg
    41761 cgaacttcac gctgagcgat ggcgacaaag cggttactga tgccgatggc aaagcgaaag
    41821 tcacgctgaa aggtacaaaa gcaggcgctc ataccgtgac agcatcgatg gttggcggta
    41881 agagtgagca gttggtggtg aactttactg cggatacgct cactgcgcag gttaatctta
    41941 acgttaccga ggacaatttc atcgccaata acattggaat gaccaggcta caggcaacag
    42001 tgactgatgg aaacggcaac ccagtagaag gtataaaagt taatttccgc ggaacctccg
    42061 tcacgctaag cagcaccagc gttgaaacgg atgatcaggt tttcgctgaa attcttgtga
    42121 caagcaccga ggtcggacta aaaacagttt cagcctctct ggcagataaa cctactgaag
    42181 tcatctcgcg attactgaat gccaaggtag atgttaattc tgcgacgatt accagtcagg
    42241 agatacctga aggtcaggtc atggtcgcac aagacatagc agttaaagct cacgttaacg
    42301 accagtttgg caacccggtg acgcatcaac ccgcgacatt cagtgcagcg ccatcctcgc
    42361 aaatgatcat cagccagaat acggtctcta ctaatacgca gggagtagcc gaggtcacca
    42421 tgacgcctga aagaaacggc tcgtatactg taaaagcatc cctagcgaat ggagcttcac
    42481 ttgagaaaca actggaggct attgatgaaa aactgacact cacgtccagt ccgcttatcg
    42541 gtgtcaatgc tcctaaaggc gctactctga cggcaacgct aacctctgca aatggcactc
    42601 cagtggaggg tcaggtcatc aactttagcg taacgctaga aggtgcgacg ttaagtggcg
    42661 gaaaagtgag aaccaactct tcaggtcagg ctccagtcgt tctgaccagc aataaagtcg
    42721 gtacatatac ggtgactgca tcgttccata acggcgtcac aatacagaca cagacaaccg
    42781 tgaaggttac tggcaaccca agcaccgcac atgttgctag cttcatcgct gatccgtcga
    42841 ctatcgccgc caccaacagt gatttaagta ccttaaaggc aacggttgag gatggcagtg
    42901 gtaacctgat cgaaggtctc actgtgtact tcgccttaaa aagcggctcc accacattaa
    42961 cgtcattaac agcggtgacc gatcaaaacg gaatcgcgac aacaagcgtg aaaggtgaga
    43021 taacggggag cgtcactgta agcgcagtca caagcgcagg tggaatgcaa acagtagata
    43081 tatcgctagt ggcaggccca gcagacgcct cgcagtccat ccttaagaac aaccagtcat
    43141 cactgaaagg ggactttacc gatagtgctg agctacatct tgttctgcac gatatatcag
    43201 gcaatccgat caaagtttct taagggatag aatttgtgca atcaggtaca aacgtgccct
    43261 atatgaaaat tagcgcaatt gattacagtc aaaatatcaa cggtgattac aaagctactg
    43321 ttacaggagg cggagagggt atcgcaacgc tgatccccgt attgaatggt gttcatcagg
    43381 cgggtctgag taccacgata gaattcatta gcgcggaaac cagaccaatg acgggtacag
    43441 tgtcagtgaa tagtgcaaac ctaccgacag cttcattccc ttcgcaggga tttaccgggg
    43501 cgtattatca gttgaataat gacaactttg caccaggaaa aacggcggct gattattcgt
    43561 tttcaagctc ggcctcctgg gtaggcgttg atgctactgg taaagtgaca ttcaaaaatg
    43621 acggtgatag caatactgta ataatcacag cgcccccaag atccggtggt gctatttacc
    43681 aaacagttcc tcctgaatcg cgttcggtgt gactatgatc attttcggtg agtattgctt
    43741 tgatccaaag gctggttttc tgtgatgatt gaccagcctc agagtattga tgacaccacc
    43801 gttcataaca gatcggcatt actatgcata agtactgcct caaattctcg ccaaaacact
    43861 ttttaatgcc tttgcgaatt catccagact tatattgtca attttttgcc caacgacgac
    43921 tgactccgaa acatcggcca ttgcgggcca tcgcgttttg taatcagcat aaattgcaac
    43981 aacaggggta ttataggcac ttgccatatg taatattgag gtgtcaggag ttattgcaat
    44041 ataagcatct tttatgattg ctgctgaacg tttaatcgag ggtgataagg ataaacggta
    44101 gacattatta taacaatcca ctaatgccct ggctttatct tcccctttgg gtccataaac
    44161 gatcactatt ggaatatctg tttcactttg aatttttgct attagatttt ctgcaatcga
    44221 taatgaaaat gtacgttcct gcgagctacc gtctaaattg agcgcaatgt aagatcctaa
    44281 cgatcgcatt tcctcgcgta cctcatcgag tacatcatcg cttaatggca attcatatat
    44341 tgacctgact gccggaaaac ccgcctcacg catcataaat gcccatgtca tgggtacagg
    44401 agtccggaga ttctgatcca tccgggacgc gttcttacac aaaggggaat agcacttcat
    44461 ggttaaaccg acgacctgga aattcgtttt tgctttcaac tgactgataa atatcatcgt
    44521 tttcaggttc tttttacgca tcgcctctat acaaagatcc ggcgtaccgt attgcagtgt
    44581 tatgttcttc gctaaatctt ttatttcttt taatgttgcg tgatcctgca tagtcattgt
    44641 gactaatgtt aatttagtct gttcaagttt gagcgcatta aagacttcta aattaattgt
    44701 cgacgtaaca attaatagat gcttaatttt atgcaattca agcgcccgaa taac
//
LOCUS       DAZGJT010000029        43543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZGJT010000029 DAZGJT010000000
VERSION     DAZGJT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.029"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            318..1766
                     /gene="uacT"
                     /locus_tag="ACHKFK_001750"
     CDS             318..1766
                     /gene="uacT"
                     /locus_tag="ACHKFK_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO9052106.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     IASGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1768..1893
                     /gene="yqfG"
                     /locus_tag="ACHKFK_001751"
     CDS             1768..1893
                     /gene="yqfG"
                     /locus_tag="ACHKFK_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO9052107.1"
                     /translation="MNLLMRAIFSLLLIFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1890..1961)
                     /locus_tag="ACHKFK_001752"
     CDS             complement(1890..1961)
                     /locus_tag="ACHKFK_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052108.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2016..2564
                     /gene="idi"
                     /locus_tag="ACHKFK_001753"
     CDS             2016..2564
                     /gene="idi"
                     /locus_tag="ACHKFK_001753"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO9052109.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEEAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLAAVLRGIDATPWAFSP
                     WMVMQATNREARKRLSAFTQLK"
     gene            complement(2607..4124)
                     /gene="lysS"
                     /locus_tag="ACHKFK_001754"
     CDS             complement(2607..4124)
                     /gene="lysS"
                     /locus_tag="ACHKFK_001754"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9052110.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4134..5232)
                     /gene="prfB"
                     /locus_tag="ACHKFK_001755"
     CDS             complement(join(4134..5156,5158..5232))
                     /gene="prfB"
                     /locus_tag="ACHKFK_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO9052111.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5323..7056)
                     /gene="recJ"
                     /locus_tag="ACHKFK_001756"
     CDS             complement(5323..7056)
                     /gene="recJ"
                     /locus_tag="ACHKFK_001756"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO9052112.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDPQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7062..7772)
                     /gene="dsbC"
                     /locus_tag="ACHKFK_001757"
     CDS             complement(7062..7772)
                     /gene="dsbC"
                     /locus_tag="ACHKFK_001757"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO9052113.1"
                     /translation="MKKGFMLFTLLAAFSGFVQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            complement(7797..8693)
                     /gene="xerD"
                     /locus_tag="ACHKFK_001758"
     CDS             complement(7797..8693)
                     /gene="xerD"
                     /locus_tag="ACHKFK_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO9052114.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8805..9326
                     /gene="fldB"
                     /locus_tag="ACHKFK_001759"
     CDS             8805..9326
                     /gene="fldB"
                     /locus_tag="ACHKFK_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO9052115.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDAPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9366..9773)
                     /gene="ygfX"
                     /locus_tag="ACHKFK_001760"
     CDS             complement(9366..9773)
                     /gene="ygfX"
                     /locus_tag="ACHKFK_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO9052116.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     SGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9754..10020)
                     /gene="sdhE"
                     /locus_tag="ACHKFK_001761"
     CDS             complement(9754..10020)
                     /gene="sdhE"
                     /locus_tag="ACHKFK_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO9052117.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10263..11243
                     /gene="ygfZ"
                     /locus_tag="ACHKFK_001762"
     CDS             10263..11243
                     /gene="ygfZ"
                     /locus_tag="ACHKFK_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO9052118.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLTLNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLKGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLRIEPLPYSLEE"
     gene            complement(11320..11979)
                     /gene="yqfA"
                     /locus_tag="ACHKFK_001763"
     CDS             complement(11320..11979)
                     /gene="yqfA"
                     /locus_tag="ACHKFK_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO9052119.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12143..12454)
                     /gene="yqfB"
                     /locus_tag="ACHKFK_001764"
     CDS             complement(12143..12454)
                     /gene="yqfB"
                     /locus_tag="ACHKFK_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO9052120.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELKKVIADIYPDQTQFYVIEFKC
                     L"
     gene            12499..13932
                     /gene="bglA"
                     /locus_tag="ACHKFK_001765"
     CDS             12499..13932
                     /gene="bglA"
                     /locus_tag="ACHKFK_001765"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO9052121.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVVPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMIDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAAHRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQKPLFIVENGFGAYDKVEDDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGENL"
     gene            complement(14098..16971)
                     /gene="gcvP"
                     /locus_tag="ACHKFK_001766"
     CDS             complement(14098..16971)
                     /gene="gcvP"
                     /locus_tag="ACHKFK_001766"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO9052122.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDIASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLDID
                     TLDKDVAHDSRSIQAAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(17089..17478)
                     /gene="gcvH"
                     /locus_tag="ACHKFK_001767"
     CDS             complement(17089..17478)
                     /gene="gcvH"
                     /locus_tag="ACHKFK_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO9052123.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(17478..18596)
                     /gene="gcvT"
                     /locus_tag="ACHKFK_001768"
     CDS             complement(17478..18596)
                     /gene="gcvT"
                     /locus_tag="ACHKFK_001768"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO9052124.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYSQEMDETISPLAANMGWTIAWEPADRDFIGREALEAQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVARFTFLEID"
     gene            18887..18970
                     /locus_tag="ACHKFK_001769"
     CDS             18887..18970
                     /locus_tag="ACHKFK_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052125.1"
                     /translation="MSKNTKSKIHNIRKYNAKTEVKLVYFK"
     gene            complement(19044..20246)
                     /gene="ubiI"
                     /locus_tag="ACHKFK_001770"
                     /gene_synonym="visC"
     CDS             complement(19044..20246)
                     /gene="ubiI"
                     /locus_tag="ACHKFK_001770"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO9052126.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSVINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYN
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEALRMQQASEDEFNRALNIAFDNRLGLCKVESERQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(20269..21447)
                     /gene="ubiH"
                     /locus_tag="ACHKFK_001771"
                     /gene_synonym="visB"
     CDS             complement(20269..21447)
                     /gene="ubiH"
                     /locus_tag="ACHKFK_001771"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO9052127.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALSVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATVCGVDWQQDPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAAKP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWTPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(21444..22769)
                     /gene="pepP"
                     /locus_tag="ACHKFK_001772"
     CDS             complement(21444..22769)
                     /gene="pepP"
                     /locus_tag="ACHKFK_001772"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO9052128.1"
                     /translation="MSDISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEHAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITALAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLENSLRLYRPGTSIQEVTGEVVRIMVSGLVKLGILKG
                     EVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(22795..23373)
                     /gene="ygfB"
                     /locus_tag="ACHKFK_001773"
     CDS             complement(22795..23373)
                     /gene="ygfB"
                     /locus_tag="ACHKFK_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO9052129.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLIHDLTNEGMAFGHELAQALRKMHSATGDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            23541..23870
                     /gene="zapA"
                     /locus_tag="ACHKFK_001774"
     CDS             23541..23870
                     /gene="zapA"
                     /locus_tag="ACHKFK_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9052130.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            23912..24095
                     /gene="ssrS"
                     /locus_tag="ACHKFK_001775"
     ncRNA           23912..24095
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKFK_001775"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            24116..24718
                     /gene="fau"
                     /locus_tag="ACHKFK_001776"
     CDS             24116..24718
                     /gene="fau"
                     /locus_tag="ACHKFK_001776"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO9052131.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKMQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24820..24879)
                     /gene="ibsC"
                     /locus_tag="ACHKFK_001777"
     CDS             complement(24820..24879)
                     /gene="ibsC"
                     /locus_tag="ACHKFK_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO9052132.1"
                     /translation="MMRLVIILIVLLLISFPAY"
     gene            complement(25108..26340)
                     /gene="serA"
                     /locus_tag="ACHKFK_001778"
     CDS             complement(25108..26340)
                     /gene="serA"
                     /locus_tag="ACHKFK_001778"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9052133.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(26596..27255)
                     /gene="rpiA"
                     /locus_tag="ACHKFK_001779"
     CDS             complement(26596..27255)
                     /gene="rpiA"
                     /locus_tag="ACHKFK_001779"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO9052134.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(27311..27541)
                     /locus_tag="ACHKFK_001780"
     CDS             complement(27311..27541)
                     /locus_tag="ACHKFK_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9052135.1"
                     /translation="MHFAQQVRALVVLNGVALLPQFACKQGLANGELVRLFAPWSGIP
                     RPLYALFAGRKGMPAIARYFMDELTTRLANGV"
     gene            27682..28575
                     /gene="argP"
                     /locus_tag="ACHKFK_001781"
     CDS             27682..28575
                     /gene="argP"
                     /locus_tag="ACHKFK_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO9052136.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            28778..29872
                     /locus_tag="ACHKFK_001782"
     CDS             28778..29872
                     /locus_tag="ACHKFK_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HFO9052137.1"
                     /translation="MSNVLVWQQLANNELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDFDNPRVAGDVGKAGVAIDTVEDMKELFDQIPLDKMSVSMTMNDAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKQSMRIIADIIAWCS
                     GNMPRFNNISISGYHMGEAGANCVQQVAFTLADRIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLGAARYLWSEAVSGFGAQDPYNNVIRTTIDHCAHPMYRDYLHRYL
                     ENASGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(29869..30765)
                     /gene="ygfI"
                     /locus_tag="ACHKFK_001783"
                     /pseudo
     CDS             complement(29869..30765)
                     /gene="ygfI"
                     /locus_tag="ACHKFK_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(30923..31663)
                     /gene="yggE"
                     /locus_tag="ACHKFK_001784"
     CDS             complement(30923..31663)
                     /gene="yggE"
                     /locus_tag="ACHKFK_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO9052138.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFNRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQPPAKTPAAH"
     gene            complement(31756..32391)
                     /gene="argO"
                     /locus_tag="ACHKFK_001785"
     CDS             complement(31756..32391)
                     /gene="argO"
                     /locus_tag="ACHKFK_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO9052139.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(32530..33390)
                     /gene="mscS"
                     /locus_tag="ACHKFK_001786"
     CDS             complement(32530..33390)
                     /gene="mscS"
                     /locus_tag="ACHKFK_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO9052140.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPARRNEFIIGVAYDSDIDQVKQILTDIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(33581..34660)
                     /gene="fbaA"
                     /locus_tag="ACHKFK_001787"
     CDS             complement(33581..34660)
                     /gene="fbaA"
                     /locus_tag="ACHKFK_001787"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9052141.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKENEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(34875..36038)
                     /gene="pgk"
                     /locus_tag="ACHKFK_001788"
     CDS             complement(34875..36038)
                     /gene="pgk"
                     /locus_tag="ACHKFK_001788"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO9052142.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(36088..37107)
                     /gene="epd"
                     /locus_tag="ACHKFK_001789"
     CDS             complement(36088..37107)
                     /gene="epd"
                     /locus_tag="ACHKFK_001789"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO9052143.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37479..37910
                     /locus_tag="ACHKFK_001790"
     CDS             37479..37910
                     /locus_tag="ACHKFK_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO9052144.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVLSQLRAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQENTALLSGKFEVIALNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGCLEELAFSRQLCALSGYDELHISPVK"
     gene            37933..38511
                     /locus_tag="ACHKFK_001791"
     CDS             37933..38511
                     /locus_tag="ACHKFK_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO9052145.1"
                     /translation="MARRHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            38512..39219
                     /locus_tag="ACHKFK_001792"
     CDS             38512..39219
                     /locus_tag="ACHKFK_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO9052146.1"
                     /translation="MHPFTSLTLWALAACTTLILPTQTILPVYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWAHAITLWLRILAIVSTSQL
                     WMQYVPVQRFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRINNRRTTLWAPADSTLQRVARY
                     TMILLMLAEFGAWIWLR"
     gene            39207..39884
                     /locus_tag="ACHKFK_001793"
     CDS             39207..39884
                     /locus_tag="ACHKFK_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9052147.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGRLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEKEIMARIDAALALTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPKASEMLLQRLQHWALERGSLIILFERHHTPFLNHCQQAWQLQN
                     GALQPLC"
     gene            39878..40555
                     /locus_tag="ACHKFK_001794"
     CDS             39878..40555
                     /locus_tag="ACHKFK_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9052148.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTADTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LILQKCPADEITQRTNAALQCCQLADTASAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PSDDIHP"
     gene            complement(40527..41240)
                     /locus_tag="ACHKFK_001795"
     CDS             complement(40527..41240)
                     /locus_tag="ACHKFK_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO9052149.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDVHQLRPFKGA
                     PETFNVAKLAENLCRVVEGDCTWPQYDRQKHDPVEDVLHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFIKAPASALRERLVGRKLAGGLSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLMD"
     gene            complement(41237..41746)
                     /gene="fumE"
                     /locus_tag="ACHKFK_001796"
     CDS             complement(41237..41746)
                     /gene="fumE"
                     /locus_tag="ACHKFK_001796"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO9052150.1"
                     /translation="MATLTEDDVLEQLNAQDNLFSFMKIAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPRFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(41768..42083)
                     /locus_tag="ACHKFK_001797"
                     /pseudo
     CDS             complement(41768..42083)
                     /locus_tag="ACHKFK_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
     gene            complement(42080..43357)
                     /gene="yggP"
                     /locus_tag="ACHKFK_001798"
     CDS             complement(42080..43357)
                     /gene="yggP"
                     /locus_tag="ACHKFK_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9052151.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVNVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPGG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QMLLPVELAASKGIELVYMNTKGMSDPVQTLRALTDDAGFDDVFVYAAVPSVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPETVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LAWLVEKTHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(43372..>43543)
                     /locus_tag="ACHKFK_001799"
     CDS             complement(43372..>43543)
                     /locus_tag="ACHKFK_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052152.1"
                     /translation="SITENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
BASE COUNT        10510 a        11647 c        10776 g        10610 t
ORIGIN      
        1 cccctcaatc tatgccgctt tcattatttc aacattgccg atcaaaagaa tgctatccag
       61 ataaaaacga ttaaatgcga gagtgcgaca tgccagaatg attaatgaaa tatcaatatg
      121 agaaaattac agataattcc attgtcaggt tctcttctca ttttcgtggt ttaaccacga
      181 ttatgtgatt gcctccgcaa ttcctgtctc taactcccct tcctcgcaaa aactggcact
      241 ccacgagcat gtgtttagac agtttcatga acataaacgg ttgcttttta ctctggcggg
      301 cgaaaggaga aacactgatg agcgccatag attcccaact tccctcatct tctgggcaag
      361 accgcccaac tgatgaggtt gaccgcatat tatcacctgg aaagctgatc atactcggtc
      421 tgcaacacgt ccttgtcatg tacgcaggtg cagtcgctgt tcctcttatg attggtgacc
      481 gactcggcct atcaaaagaa gctattgcga tgctcatcag ctcggatctc ttttgctgcg
      541 ggatcgtcac attattgcaa tgtatcggta tcggccgctt tatggggatc cgcctgccgg
      601 tgattatgtc ggtgaccttc gccgctgtaa caccaatgat agctattggt atgaacccgg
      661 atatcggcct gctggggata ttcggtgcca ctatcgccgc gggttttatc accacattat
      721 tagcgccact tatcggtcgc ttgatgcctt tattcccgcc actggttacc ggtgtggtta
      781 ttacttctat cgggcttagc atcattcagg tgggtattga ctgggccgcc ggaggtaaag
      841 ggaatccgca atatggtaat cccgtttatt taggtatctc ctttgccgtc ttaattttta
      901 tcttgctcat tactcgctat gcgaaaggat ttatgtccaa cgtcgccgta ttactgggga
      961 ttgtatttgg ctttttactt tcgtggatga tgaatgaagt caatttatcc gggctacatg
     1021 atgcttcatg gtttgcgatt gtcacgccga tgtcatttgg tatgccgatt ttcgatcccg
     1081 tttccattct gaccatgact gccgtgttaa tcatcgtgtt tatcgagtcg atggggatgt
     1141 tcctggcact gggtgaaata gtcggtcgta aactctcttc acacgatatt attcgcgggc
     1201 tgcgtgtcga tggtgtaggg acaatgatag gcggaacgtt taacagcttc ccccacacgt
     1261 cattttctca aaacgttggc ctggttagcg tgacgcgcgt tcatagccgc tgggtgtgca
     1321 ttgcttccgg tatcattttg atcctgttcg gcatggtgcc caaaatggcg gtgctggtgg
     1381 cctccattcc gcaatttgtg ctgggcggcg cggggctggt gatgttcggc atggtactgg
     1441 cgacagggat tcgaattctg tcgcgctgta actacaccac taaccgttac aacctctata
     1501 ttgtggcgat cagtctcggt gttggcatga cgccaacgct ctctcacgat ttcttttcta
     1561 agttaccggc cgtactgcaa ccgttgctgc atagcggcat tatgctcgca acccttagcg
     1621 ccgttgtgct gaacgtcttc tttaatggct accagcatca tgctgacctg gtgaaggaat
     1681 ccgtctctga taaagattta aaagtcagga cagtacgtat gtggcttctg atgcgcaagc
     1741 tgaagaaaaa tgagcatgga gaataatatg aatcttttaa tgcgcgctat atttagtctg
     1801 ctgttgattt ttactctctc tattcctgtc atttctgatt gtgttgcaat ggccattgaa
     1861 agtcgcttca aatatatgat gctgcttttt taaatggttt ttacctgtcg acatccgctc
     1921 aaaacgggcg gttgtcgata aacgctcact tggttaatca tttcactctt ctattatcta
     1981 taatgatgag tgatcagaat tacatgtgag aaattatgca aacggaacac gtcattttat
     2041 tgaatgcaca gggagttccc acgggtacgc tggaaaagta tgccgcacac acggcagaca
     2101 ccctcttaca tctcgcgttt tccagttggc tgtttaatgc caaagggcaa ttattagtta
     2161 cccgccgcgc ccttagcaaa aaagcatggc ctggcgtgtg gactaactcg gtttgtgggc
     2221 acccacaact gggagaaagc aacgaagagg cggtgatccg ccgttgccgt tatgagcttg
     2281 gcgtagaaat tacgcctcct gaatctatct atcctgactt tcgctaccgc gccaccgatc
     2341 cgaatggcat tgtggaaaat gaagtgtgtc cggtatttgc cgcacgcacg accagtgcgt
     2401 tacagatcaa cgatgatgaa gtgatggatt atcaatggtg tgatttagca gcggttttac
     2461 gcggtattga tgctacgccg tgggcgttca gtccgtggat ggtgatgcag gcgacaaatc
     2521 gcgaagccag aaaacgatta tctgcattta cccagcttaa ataaaaaacc ccgacggttg
     2581 ccggggttgt gagcataacg taatgcttat tttaccggac gcatcgccgg gaacagaata
     2641 acgtcgcgga tggtatggct gttggtgaac agcattacca tacggtcgat accaattccc
     2701 agacctgctg tcggcggtaa gccatgttcc agtgcggtga cataatcttc gtcgtagaac
     2761 atcgcttcgt cgtcacctgc atctttcgcg gcaacctgat ccaggaagcg ctgcgcctga
     2821 tcttccgcgt cattcagctc gctaaagccg ttaccgattt cacgccctcc aatgaagaac
     2881 tcaaagcggt ctgtgatttc cgggttaacg tcgttacgac gcgccagagg agaaacttct
     2941 gccggatatt cagtaatgaa ggtcggctgg atcagatgag cttctgccac ttcttcgaag
     3001 atctcggtaa cgatacggcc cagaccccaa ctcttctcaa cgtggatgcc gatagattca
     3061 gcaatcgctt tcgcagagtc gaagttgtcc agatccgcca tgtcagtttc cgggcgatat
     3121 ttcttgatcg cttcacgcat ggtcagtttt tcgaacggtt taccgaagtc cagcgttacg
     3181 tcgccgtagg tcacttccgt cttaccgaga atatcctgtg ccagagtacg gaacagcgat
     3241 tcggtcagct cgatcagatc tttgtaatct gcgtaagcca tatagagttc catcatggtg
     3301 aactctgggt tatgacgtac ggaaatacct tcgttacgga agttacggtt gatttcgaat
     3361 acacgctcga agccaccgac caccagacgc ttgaggtaca gttccggcgc gatacgcagg
     3421 tacatgtcga gatccagcgc gttatggtga gtgataaacg gacgcgcagc ggcaccgcca
     3481 gggatcacct gcatcatcgg cgtttcaact tccataaagc cgcgattcac catgaactgg
     3541 cgaataccag agaggatctg cgagcgcact ttaaaggtgt tgcgggattc atcgttagag
     3601 atgagatcca gataacgctg acgatagcgc gcttcctgat cctgcaagcc gtggaattta
     3661 tccggcagcg gacgcagtgc tttggtcagc agacgcagct cggtacagtg gatagacagt
     3721 tcgccggttt tggttttgaa cagtttacct ttcgcgccga ggatatcgcc gaggtcccat
     3781 tttttgaact gctcgttgta aacgccttcc ggcagatcgt cacgggcaac gtacaactga
     3841 atgcgaccgc caacgtcctg cagagtaacg aaagacgctt tacccataat acgacgggtc
     3901 atcatgcggc cagcaacggc gacttcgatg ttcagcgctt ccagttcttc gttctcttta
     3961 ccgtcgaatt ctgcgtgcaa ttggtcagag gtatgatcgc gacggaaatc gttcgggaag
     4021 gcaatcccct gctcacgcag gttcgccagc ttctcacgac gcgttttcag ttcattgtta
     4081 agatcgacta ccgcgtcagc gccctgtgcg tgttgttcag acatgttggt tcctcataac
     4141 cctgctttca aacttgcttc gataaattga tccaggctgc cgtccagcac ggcctgcgtg
     4201 ttgcgggttt ctaccccggt acgcagatct ttaatgcggg agtcatcaag gacataagaa
     4261 cgaatctggc tgccccagcc gatgtcggat ttattatctt ccatcgcctg tttctcggca
     4321 tttttcttct gcatctccag ttcataaagc ttcgctttca tctgcttcat ggcctggtct
     4381 ttgttcttgt gctgggaacg gtcgttctgg cattgggtca cgatcccggt tgggatgtga
     4441 gtaatacgca ccgcagattc ggtacggtta acgtgctgac cgcccgcgcc agatgcgcga
     4501 taaacgtcga tgcgcagatc cgccggattg atttcgatat cgatatcatc atcaacttcc
     4561 gggtaaacaa acgctgaact aaacgacgtg tggcgacgac cgccggagtc aaacgggctc
     4621 ttacgcacca ggcggtgaac gccggtttct gtacgcagcc agccataagc gtaatcgccg
     4681 gagattttga tcgtcacgga tttaataccc gccacttcac cttccgactc ttcgatgatt
     4741 tcagttttga aaccacgcga ttcagcccag cgcagataca tacgctcaag catgctcgcc
     4801 cagtcctgtg cttccgtacc gccagacccc gcctgaatat cgaggtagca gtcggcgctg
     4861 tcatattcgc cagagaacat acggcggaac tcaagctgcg ccagtttttc ttccagggcg
     4921 tcgagttcag caacggcttc gttaaaggtt tcttcgtcgt cagcttctac agccagttcc
     4981 agcagaccag aaacatcttc cagcccctgt ttcatttggt cgagggtgtc gacaacggct
     5041 tcgagggagg aacgctcttt acccagcgcc tgtgcgcgtt cgggttcgtt ccagacatcc
     5101 ggctgttcca gctcggcgtt tacttcttcc agacgctctt tcttggcgtc atagtcaaag
     5161 atacccccta agaacgtcgg agcgttccgt gaggtcctga atgcgattat ttaccggatt
     5221 aatttcaaac atggtctgat ttcttttatt gagctagtca aaatgcggtg ataagagcgg
     5281 gattgtaccc aatccacgct cttttttata gagaagatga cgctaaattg gccagatatt
     5341 gtcgatgata atttgcaggc tgcggttgcc gcgaaactcg ttgatatcga gcttgtaagc
     5401 cagttgcact tcgcgcacac cgttatccgg ccagagggcg gtatcgacat taaaagcaat
     5461 cccgtccagc agtggaccgc cgccgaccgg ttcgaccatg actttcaaat gacgttcgcc
     5521 caccagccgc tgttgcagca gacgaaaatg accgtcaaac agcggctccg ggaacatctg
     5581 cccccacggg ccagcatcgc gcaacagctg cgccacttcc atggtcattt cggccggact
     5641 taacggaccg tccgacacca cttcgccttg caatagcgaa gggtccaacc actccgtaac
     5701 cagttcgcca aaccgttgtt gaaagagttc gaatttatcc tcttccagcg acaaacccgc
     5761 cgccatcgca tgaccgccaa acttgagcat catgccgggg tagagtgtgt ctaatcgctc
     5821 cagcgcatca cgcatatgca gcccctgaat ggagcgaccg gaacctttca gcgtaccatc
     5881 accagctggc gcaaaggcga taaccggacg gtgaaaacgc tctttgatac gcgatgccag
     5941 aatgccaaca acgccctgat gccattcggg gtgatacatt gccagcccac ctggcagcgt
     6001 gtcgcggcta cgttccagct tctcgcacag agtgagcgct tcaacctgca ttccctgttc
     6061 gatctcttta cgtgtttggt ttagtgcatc gagttcattc gccagcacac gcgcttcgcc
     6121 aatgttgtca cacaacaaca gcgccacgcc aacggacata tcgtccagtc gcccggcagc
     6181 attgagacgt ggccccagcg caaaacctaa atcgctggcg gcgagttttt gtggatcgcg
     6241 attggccact tccagcagcg ctttaatccc cggacggcac tttccggcac ggatgcgact
     6301 catcccctgc caggtcagaa tgcgattatt agcgtccagc ggcacgacgt ccgccactgt
     6361 ccccaacgct accagatcca gcagttctgc cagattagga attgcgatgc cgcgctcatc
     6421 aaaccagccc tgatcgcgca aaaaggtgcg cagcgccagc atcagataaa acgcaacacc
     6481 cacgcctgcc agtgatttcg acgggaaatt acagtcgcgc aagttagggt taatgattgc
     6541 ttccgctgcg ggtaatgtgt cgcctggcaa atggtgatcg gtaacaataa ccggaatgcc
     6601 caacgaacga gcgtgttcaa cccccgcatg ggaggaaata ccgttatcca ccgtgacaat
     6661 taactgcgcg ccacgggcat gagcctgatc gactacttcc gggcttaagc cgtaaccgtc
     6721 ttcgaaacgg tttggaacca ggtagtcgat attgctgcaa ccaagcgagc gcatcgccag
     6781 cacgcttaga gccgtgctgg tggcaccgtc ggcgtcgaaa tcaccgacca caataatccg
     6841 cgtgccttcg cgaaaagcgt tgtaaaggat ctcaacggcc ttttcgacgc cgctcagttg
     6901 ctgccagggc agcatgcctt taacactgcg ttccagttcc tgcgcgctac gcacaccccg
     6961 gctggcatat aaacggcgca gcaagggagg caattcagcg ggcaagtcgg ccgtttcatc
     7021 gacttcacgg cgacgaagtt gtatctgttg tttcacgcga attatttacc gctggtcatt
     7081 ttttggtgtt cgtcgaggaa ttctttcatg tctttcggcg gctggtaacc tggaacaagt
     7141 gtgccattgc tcagcacaac tgccggagta ccgctaacgc caagctggac gccaagtgcg
     7201 taatggtcgg caatatccac gtcgcagctg gctggtgcga cgcttttacc tgccatcaca
     7261 tcatcaaacg ctttgttttt atctttcgca caccagatag ctttcatttc tttctctgca
     7321 tcgctgtcca gcccctggcg cgggaaagca agataacgca cggtgatccc cagcgcgtta
     7381 tagtctgcca tttgttcatg cagtttgtgg cagtaaccac aggtaatatc agtaaacacg
     7441 gtgatgacgt gtttttcctg cggcgcttta taaacgatca tctctttttc aagcgcattc
     7501 aactgcttta acagcatctt attggtgaca ttgaccggag ccgtgccact aacgtcatac
     7561 attggcccct gaatgatatg tttaccatca tcggtgatgt acaacacacc gctgttagtc
     7621 agaactgtct tcatgccagc tacgggcgcg ggctgaatat cgctgctttt gatgcccatt
     7681 ttggctaacg tttgttgaat tgccgcgtca tcagcctgaa caaagcctga aaacgctgcc
     7741 aacaaggtaa ataacataaa acctttcttc ataaattttc ctgttctttt cagacatcac
     7801 gcccgcgggt gatgctgttg atgaagttgc cgcagacgct cggtagcgac atgcgtataa
     7861 atttgcgtgg tggagagatc gctgtggccc agtagcatct gcaccacgcg caaatccgca
     7921 ccatgattta ataagtgagt ggcaaaagcg tgacgcaaca catgcggtga cagcttttcg
     7981 ctgtcgatac ccgccagcac ggcataatgt ttaatgcggt gccagaaggt ctgtcgcgtc
     8041 atctgctgcg cacgctggct gggaaacaac acgtcaattg acacaccatt caacagccac
     8101 ggacgcccat gttccagata ggtttccagc cagtaaaccg cctcttcacc taacggcacc
     8161 aggcgctctt tgttgccttt accaatgacc cgcaccacgc cctgacgcag gctgatatca
     8221 ctcattgtca gcccgaccag ttcagagaca cgcagtccgg tagcatacaa cacttcaagc
     8281 atggctttat cgcgtagctc cagtggctga tcaattaatg gtgcctgtaa taaacgttcg
     8341 acctgcgctt cgcttaaatc ttttggcaaa cgctggggca atttcggtga agcgagatgc
     8401 gcactgggat catcttcacg aaacttttcg cgataaagat actggaacaa tcggcgcact
     8461 gcactcagca aacgcgctga gctggtggct ttatacccgc cctccagccg ttctgccagt
     8521 aatgcctgca aatcgtcact ttgcgccgtc gccagcgtca acccgcggtg atgcaaccac
     8581 tccaccatca ttgacagatc gcgacggtaa gcgttcaacg tattttcagc cagatttttt
     8641 tccagccaca gagcatcaag aaactgctcg atgcgtgcca gatcctgttt cacttgcgcc
     8701 ccttatggtc actcatttga tccattatgc cttattgtgc cgtgactaaa gcgattctga
     8761 tacactagcc gcaaaagcca cagcagaatc gagaagctta cgttatgaat atgggtcttt
     8821 tttacggttc cagcacctgt tacaccgaaa tggcggcaga aaaaatccgc gatattatcg
     8881 gcccagaact ggtgacctta cataacctca aggacgacgc cccgaaatta atggagcagt
     8941 acgatgtgct cattctgggt atcccgacct gggattttgg cgaaatccag gaagactggg
     9001 aagccgtctg ggatcagctc gacgacctga acctcgaagg taaaattgtt gcgctgtatg
     9061 ggcttggcga tcaactggga tacggcgagt ggttcctcga tgcgctcggt atgctgcatg
     9121 acaaactctc gaccaaaggc gtgaagttcg tcggctactg gccaacggaa ggatatgaat
     9181 ttaccagccc gaaaccggtg attgctgacg ggcaactgtt cgtgggtctg gcgctggatg
     9241 aaactaacca gtatgacctt agcgacgagc gtattcagag ctggtgcgag caaatcctca
     9301 acgaaatggc agagcattac gcctgatgtc actgacggct tagcacatat gctttgccgt
     9361 catctttatc tttgcgtctc ttgttgtaac aatatccgcc gtaaatcccg ccattcggct
     9421 tcgtccatgc tgtcagctgc cagccataaa tgttgccgtt ttccgctgtc agaacgtaaa
     9481 cgcagcatca tgccgctctt aatcatccac ggcgctttga cgatgctcca ctcctgccct
     9541 tgccaacgca aacgcccgtc catcaacaag cgaatttccc cctggcgagc attaatgcgc
     9601 cgctggctgc gaacgcaatc aaacaccacc agcgaaagta acaccatcca taacggggtg
     9661 taactgagcg gccagggcat gagtaaaata acagcggcaa ccagcccatg aatcagcaag
     9721 gaaagccact gtgcgcgcca ggagacgcgc aaatcagatt gccacaggac cacgttcccg
     9781 gttccgtgtc tgaatgagtc ggaccatcat ttccagttct gcatcggccg gtttaccgtg
     9841 attcatcagc cagttaaaca ggtccggatc gtcacattcc agcagacgaa taaagatgcg
     9901 tttttcgtca tcgcttaagc tgtcgtactc atgttcgaaa aacggcatga ttgaaatatc
     9961 gagttcgcgc ataccacggc ggcatgccca atgaatgcgg gctttgttgt taatgtccat
    10021 cttcttcctg tctcacgaaa atccagtacc cggctattgt aacgtgtttt tcgacttctt
    10081 ttacgggaat atcagtaaac acaatcgcga tcgcgaaatt aattcacaac aattcaaggg
    10141 cttcattttt ttggaagtcg cctcgcagaa ggcacagatc gcgtagtgaa agcacttgca
    10201 ttgcctcata gctcttttac cattagttat taatacgccg ttaagcaact caggactcta
    10261 ttatggcttt tacacctttt cctccccgtc agcctacggc ttctgcccgt ttgccactga
    10321 cgctgatgac gcttgatgac tgggcgcttg ccactattac tggcgcggac agcgaaaaat
    10381 atatgcaagg ccaggtgaca gcagatgtca gccagatgac tgaagatcag cacctgctcg
    10441 ccgcccattg cgacgccaaa ggtaaaatgt ggagcaattt acgtctgttc cgcgacggcg
    10501 atggctttgc atggattgaa cggcgcagcg tgcgtgaacc gcagctgact gaactgaaaa
    10561 aatatgcggt gttctctaaa gtgaccatcg cgccagacga cgagcgtgtg ctgcttggtg
    10621 ttgccggttt tcaggcgcgc gccgcgttgg caaatctctt tagtgaacta ccttcgagag
    10681 aaaaacaggt agtcaaagaa ggcgcgacca cgttgctatg gtttgaacac ccggcagaac
    10741 gtttcctgat cgtaaccgat gaagctactg ccaatatgct gaccgataaa ctgcgcggtg
    10801 aagcggaact gaacaatagc caacagtggc tgacattaaa cattgaagcg ggttttccgg
    10861 tgattgatgc cgccaacagc gggcagttta tcccccaggc gaccaacctc caggcgctgg
    10921 gcggtatcag ctttaagaaa ggctgctata ccggacaaga gatggtggcg cgagcaaaat
    10981 tccgtggtgc caacaaacgc gcgctctggt tgctgaaggg tagcgccagc cgactgccgg
    11041 aagctggtga agacttagag ctgaaaatgg gcgagaactg gcgtcgtacc ggtacggtgc
    11101 tggctgcggt caaactggaa gatggtcagg ttgtggtgca ggttgtcatg aataacgata
    11161 tggaaccgga cagcatcttc cgcgtgcgtg acgatgcgaa tacattgcgt atcgagccac
    11221 tgccatattc gctggaagag taaatctctg tatcgcatca ggcatttatc gcctgatgcg
    11281 acactggtgt gtcttttcag gcctacaaag gcacgccaat tacgcctgcc caatatacaa
    11341 atagatcgcc agaaagtggc acacactacc gccgagcaca aagccgtgcc agatggcatg
    11401 attgtatgga atgcgcttgc agacgtagaa aatcaccccg agcgaataaa ccacaccgcc
    11461 taccgccagt aaggtaacgc tgcccgccgc gagcttaact gccatttcat aaattaccac
    11521 cagcgacagc cagcccatcg ctagataggt caccagagat aaaattttga atcggtgcgc
    11581 gatggtcagt ttaaacagaa tacccagcaa tgccaggctc cagataacaa tcatcaaccc
    11641 gcgcgctaac ggagaatcca gccccaccag caaaaacggc gtgtaggttc cggcaatcaa
    11701 cagataaatg gcacagtggt caaatttctt cagccacatt tttgcccgtt gatggggaat
    11761 ggcgtgatag agcgtcgaag cgaggaacag caggatcata ctgccgccat agaggctgta
    11821 actggttatc gccgtggcgc tggcattgag atccaccgcc tgaaccagta gcaacaccag
    11881 cccaacgata ccaaacacca gcccaatgcc gtgactgacg ctgttggcaa tttcctctgc
    11941 cagcgaatat ccctgcttaa tgaggggctt ctgaaccata acttactccg gaaaaacgta
    12001 cacgcacatg tatacctctc cagcgtaact gagaatagtt ccagtgaaca cctgttagct
    12061 aaaataaatt ctgatttgag attaatctcc gaaaaatcat gaaattaaat gcaaaatttc
    12121 aactaacagg cgtgagttca atttaaagac atttaaattc aatcacataa aactgtgtct
    12181 ggtcgggata gatgtcggca atgacctttt tcagttcagt cagggtcata ttttcctgct
    12241 ctgcatgttt ttccgtcagc gtatccagcg ttacggttga ggttgcggtg acttcaatcg
    12301 tgcaaaaata accgtcatct tcaaaacgtc cgacacgaag cacatcaccc gttttgaagt
    12361 gcgattcaga ctcgtcgcgg atggtgatgg ttttacgccc agccagaatg tcatcctgga
    12421 aacgttgaaa aaaagtgatg tcgtttggct gcatggtaat atttcctgta agaattgact
    12481 catctggagc ctatgatagt gaaaaaactc accttaccga aagatttctt atggggcggc
    12541 gcagttgccg ctcatcaggt cgaaggcggc tggaacaaag gcggcaaagg gccgagcatt
    12601 tgtgacgttt tgaccggtgg cgcacacggc gtgccgcgcg aaatcaccaa agaagttgtg
    12661 cctggcaaat actatccaaa ccatgaagcc gttgattttt atggtcacta caaggaggac
    12721 atcaagctat ttgccgaaat gggcttcaaa tgttttcgta cttccatcgc ctggacgcgc
    12781 atttttccaa aaggcgatga agctcagcca aacgaagaag ggctgaagtt ctacgatgat
    12841 atgatcgatg aactgctgaa atacaacatc gaaccggtga tcaccctctc ccactttgaa
    12901 atgcctctgc atctggtgca gcaatatggt agctggacca accgtaaagt ggttgatttc
    12961 tttgtccgtt tcgcggaagt ggtatttgaa cgctataagc ataaagtcaa atactggatg
    13021 accttcaacg aaattaacaa ccagcgtaac tggcgtgcac cgctgttcgg ttactgctgc
    13081 tccggcgtgg tgtataccga gcatgaaaac ccggaagaga cgatgtatca ggtgctgcat
    13141 caccagtttg tcgccagcgc cctggcggtg aaagccgcgc atcgcatcaa cccggagatg
    13201 aaagtcggtt gtatgctggc gatggtgccg ctctatcctt actcctgtaa cccggacgat
    13261 gtgatgtttg cccaggagtc gatgcgcgaa cgttacgtct ttaccgatgt gcagttgcgt
    13321 ggctattacc cgtcctatgt gttgaacgag tgggaacgcc gcggatttaa catcaaaatg
    13381 gaagacggcg atctggatgt actgcgcgaa ggcacctgcg attatctcgg cttcagctat
    13441 tacatgacca acgcggtgaa ggccgaaggc ggcaccggcg atgcgatctc tggttttgaa
    13501 ggcagcgtac cgaacccgta tgttaaagcg tctgactggg gctggcagat tgatccggtt
    13561 ggtctgcgtt acgcgctttg cgagctgtat gagcgttacc agaagccgct gtttattgtc
    13621 gaaaacggtt ttggcgctta cgacaaagtg gaagatgatg gcagcatcaa tgacgattac
    13681 cgcattgatt acctgcgtgc ccatattgaa gagatgaaaa aagcggtgac ttacgatggc
    13741 gtggacctga tgggctacac gccgtggggc tgcatcgact gtgtgtcgtt caccaccggg
    13801 cagtacagca aacgctacgg ctttatctat gtgaataaac atgacgacgg tactggcgat
    13861 atgtcgcgtt cacgtaagaa gagctttaac tggtacaaag aggtgattgc cagcaacggc
    13921 gagaatctgt aattagccga tgtgcctgat gtgctccgct catcaggcct ggcactcttt
    13981 taatatattg agctcgcaat attttatatt acgcctcagc cgaaacacac aaagcgcact
    14041 ttattaacaa gcgaaaaagg cgccgaagcg cctttataag atagtcgaat cagtaaatta
    14101 ctggtattcg ctaatcggta cgcaggagca gaacaggtta cggtcgccgt aaacatcatc
    14161 cagacgtttc acggtcggcc agtatttgtc tgccacacct gccgggaata ctgcaacttc
    14221 acggctgtac ggatgcgccc actcggcgac cagttcgctt tgaatgtgcg gcgcattcac
    14281 cagcgggtta tcttccagcg gccagacacc ggctttcacc tgatcaattt ctgcgcggat
    14341 agccagcatc gcatcgataa agcgatccag ttccactttg ctttcagatt cagtcggttc
    14401 aaccatcagc gtacccgcca ccgggaacga catggtcggc gcgtggaaac cgtagtcgat
    14461 caggcgcttg gcaatatcca gctcgctgat gccggtttct tctttcagcg ggcgaatatc
    14521 gagaatacat tcgtgcgcca cgcgaccgtc gcgaccggta tacagcaccg ggaaggcatc
    14581 ctgcagacgg ctggcaatat agttggcgtt aagaattgcc acctggcttg cttttttcag
    14641 cccttctgcg cccatcatgc ggatgtacat ccagctgatt ggcaggatag aggcactacc
    14701 gaacggtgcc gcagaaaccg cgccctgacg ggttaacatg ccttcgattt gcaccacgct
    14761 atgacccggt acaaacggag ccagatgcgc tttcacaccg atcggtccca tacccggacc
    14821 accaccgcca tgcggaatgc agaaagtttt atgcaggtta aggtgggaaa cgtccgcacc
    14881 aataaagccc ggcgaggtga tgccaacctg agcgttcatg ttcgcgccat caaggtaaac
    14941 ctgaccgccg aactgatgca cgacttcaca cacttcacgg atcgtttctt catacacgcc
    15001 gtgggtagaa ggataggtca ccatgataca ggagaggtta tcgcccgcct gttccgcttt
    15061 cgcgcgcaga tcagtcagat cgatgttgcc gtttttatca cacgccacaa ccaccacctg
    15121 cattcctgcc atatgcgcgg aagccgggtt agtgccgtgc gcagaagccg ggatcaggca
    15181 gatatcgcga tgcccttcgt tgcggctttc atgataatga cgaatcgcca gcaggcccgc
    15241 gtattcgccc tgtgcgccag agttcggctg catacaaacg gcgtcgtaac cggtcagttt
    15301 caccagccag tcagccagct gcgcgatcat ctgctgataa ccttcagcct gttctggcgg
    15361 gcaaaacggg tgcagttcgg caaattccgg ccaggtgatc gggatcatct cggcggcggc
    15421 gttcagtttc atggtgcagg aacccagcgg gatcatcgcc tgattcagcg ccagatcttt
    15481 acgctccagc gagtgcatat agcgcatcat ttcggtttcg ctgtggtagc gattaaacac
    15541 cggatgggtg aggatttcgt cgtcgcgcag catcgcagcc tgaatagagc gactgtcgtg
    15601 agccacgtct ttgtccagtg tgtcgatgtc cagcccgtgg ttatcgccca acagcacgct
    15661 gaaaagctgc atcacgtttt cgcgcgtggt tgtttcatca agggtgatcc caaccgcgtt
    15721 cagaatatcg ctacgcaggt tgatttcagc cgcttcggca cgcgccagca cgcccgcttt
    15781 gtcggccact tccacacaca aagtatcgaa atagtgcgca tggcgcagct tcagaccttt
    15841 ttgttgcagg cccgccgcca ggatatcggt cagacggtgg atgcgattag cgatacgttt
    15901 caggccaacc ggaccgtgat aaacggcata caggctggca atgtttgcca gcagtacctg
    15961 ggaagtacaa atgttggagt tcgctttctc acgacgaata tgttgctcgc gagtctgcat
    16021 cgccatgcgc agagcggtgt tgccagctgc atctttcgat acaccgataa tacggcccgg
    16081 cattgagcgt ttgtattcgt ctttcgccgc aaagaatgcc gcgtgtgggc caccatagcc
    16141 catcggtacg ccaaagcgtt gcgccgaacc aaaaacaata tccgcgccct gtttacccgg
    16201 cgcagttaac agcaccagcg ccataatatc ggcggcaacg ctgaccacga ttttgcgtga
    16261 tttcagttcg ctgataagcg cggtgtagtc gtgaatttca ccagtagtgc cgacctgctg
    16321 taacagcacg ccgaagacgt cctgatggtc gagcactttt tgcgcgtcat cgacaatcac
    16381 ttcaaaacca aaggtttcgg cacgagtacg gaccacatcc agcgtttgcg gatgaacgtc
    16441 agaagcaaca aagaagcggt tggcattttt cagtttactg acgcgtttcg ccatcgccat
    16501 tgcttcggcg gcagcagtgg cctcatccag aagagaagcc gaagctatat ccagcccggt
    16561 caaatccagc gttacctgct ggaagttgag tagtgcttca aggcggccct gggagacttc
    16621 aggttgatac ggagtatacg cggtatacca gcccggattt tccagcatgt tacgcaggat
    16681 aaccggcggt agctgcacgg cggtgtaacc catgccgatg taagacgtga agcgtttatt
    16741 gcgactggca atagccttga gttctgccag tgcggcgtat tcggtcgccg gtgcgccaac
    16801 ctgcggtggt gtcgcaagct gaatatcttt cggcacaatc tggccggtca gcgcgtttaa
    16861 cgattgtgca ccaacggcat tcagcatttc ttgctgttgc gcggcgtccg gtccgatatg
    16921 gcgttcaata aaagcgccgc tgttttcaag ctggcttaac gtctgtgtca tgagcgatgg
    16981 ttcctgaaac gtgcagtgaa ttgtgaacct ctctccttgc gaagagagtg agggtgaggc
    17041 gtaaatttcc tcaccctgat cctctcccgc agaagaggaa taaagccgtt actcgtcttc
    17101 taacaatgct tcgtatgcgg ttgcatccag cagtgattcc agttcgcttt catcgctggc
    17161 tttgattttg aaaatccagc cgcctgcata cggttcgctg ttcaccagtt ccggagaatc
    17221 gctcagtgca tcgtttaccg ccacgatttc accgcttact ggcgcataaa tatctgacgc
    17281 cgcttttacc gattcggcaa ccgcgcagtc atcgcccgcg ctaaccgttg cgcccacttc
    17341 cggtaggtca acaaacacca tatcgcctaa cagctcctga gcgtgttcgg tgatgccaac
    17401 ggtgtaagtg ccgtcggctt ctttacgcaa ccattcgtgt tctttgctgt atttcagttc
    17461 tgctggtacg ttgctcatca atcaatctcc aaaaaagtaa atctcgcgac ggctttgccg
    17521 ttacgcacaa aaacaggttt cgtcacttta accggcattt cacggttgcg aatttgcaca
    17581 atcgccgttt caccaatacc ttccggcacg cgcgccagcg caatgctata acccagcgtt
    17641 ggggagaaag taccgctggt gataatgcct tcatgctggt tgccctgcgc atcagtaaaa
    17701 cgtaccggca gttcattacg cagcacgcct ttttcggtca tcaccagacc aaccagtttt
    17761 tctgtgccat gttcacgctg cgcttccaga gcttcacgac cgataaagtc acgatctgcc
    17821 ggttcccagg cgatagtcca gcccatattg gcggctaaag gagaaatagt ttcgtccatc
    17881 tcctggctat acaaattcat gcccgcttcc agacgcagcg tgtcacgcgc gcccaggcca
    17941 catggcttaa cgccagcttc caccagcgcg cgccagaaat cggccgcttt ttcattgggc
    18001 agcgcaattt cgtagcccgc ttcaccggtg taaccggtgg tggcaataaa cagatcgccc
    18061 gcctgcacgc caaagaacgg tttcatccct tccaccgcct gacgctgggc gtcattaaac
    18121 agtgtggcag cttttgcctg cgcattcggc ccttgtacgg cgatcatgga aaggtcatca
    18181 cgaacagtaa tttcgatgcc gaaaggttca gcgtgttggg taatccagga gaggtctttt
    18241 tcgcgggtgg cggagttaac aacgaggcgg aagaaatctt cagtaaagta gtagacgatg
    18301 agatcatcta tcacaccgcc agaggcattc aacatccccg agtaaagggc tttgccgctt
    18361 ttggtgagct tcgccacatc gttcgccagc agataacgta gaaactcccg ggtgcggctg
    18421 ccgcgaagat cgacgatggt catatgtgac acatcaaaca ttccggcatc ggtacgtacc
    18481 gcatgatgtt cgtcgatttg cgaaccgtaa tgcagcggca tcatccagcc gtggaaatcc
    18541 accatgcgag cgccgcaaag cgtgtgttgt tcatacaaag gagtctgttg tgccatcttg
    18601 tcctcattga ataagcgggg ctgacaactt tttcatggtg aaattatcac cacgaaaccc
    18661 agcatcggag ccactcccgg tccccaacgc aatcgttctc ttttgcctga acttaccacc
    18721 gaaacagact gttaaccata aggtaaaatt gatcatcaca ttagcttatg gttaaaaaat
    18781 gccaaaatcg caacagaata aaaaaccaaa aaacacatca gtttctatac aaagatgatg
    18841 tgatgagaaa tttaatttga ataagacaat attaagcgct aaaaaaatgt caaaaaacac
    18901 taaatcaaaa atccataaca ttagaaaata taatgcgaaa acggaggtta aattagttta
    18961 tttcaaatga ggaaaatctc ccggcgaaaa aaccgggaga tgaaaatgtg atgggtatca
    19021 aataaacagc agaagagaaa tatttaacgc agccattcag gcaaatcgtt taatcccatt
    19081 gcctgacgaa taagttgtgg cttaacgcca ggaagcgtat cagccagttt cagaccaata
    19141 tcacgcagca attttttcgc cggattagcc ccggaaaaca gatcgcggaa tccctgcata
    19201 ccagccagca tcagcgccgc gctgtgcttg cggctacgct catagcgacg caggtaaatg
    19261 tactgcccga tgtctttacc ctgacgatgc aaccgtttca gttcagcaat cagctctgca
    19321 gcatccataa agccgagatt taccccctgc cccgccagcg ggtgaatggt atgcgcggcg
    19381 tcgcccacca acaccagacg gtgcgcggca aactggcgcg cataacgccc cgtcagcggg
    19441 aacacctgac gctcgctctc aaccttgcat aagcccaggc gattatcaaa agcgatattt
    19501 aacgcgcgat taaattcgtc ttcactagcc tgctgcatcc gcagcgcttc ctctggcgac
    19561 agtgaccaga caatcgagca aagatgcgga tcgctaagcg gtaaaaaggc cagaatgcct
    19621 tcgccatgga aaacctgccg cgccaccgca tcatgcggtt cttccgtgcg aatggtcgcc
    19681 accagcgcgt gatgctgata atcccagaaa gtcagcggaa tatcggcttt gttgcgcaac
    19741 caggaattag cgccgtccgc gccaatcacc agacgcgccg ttaacatgct gccatctttc
    19801 aacgtcagga aggtttcatt ttctccccag gcgacctgct gtaattctgc gggggccaac
    19861 agagtgatat ctgacgactg ctgcgctttg ttccacagcg cgtagtgaat cactgagttt
    19921 tcaacgatat gcccaagatg gttatagccc atgctttgat cgtcaaacga gatgtgacca
    19981 aaactgtctt tgtcccacac ttccataccg tgatagcaac tggccctacg agagagaatg
    20041 tcctgccaga cgccaagacg agtcagtaat ttttcgctgg cggcattgat aaccgaaacg
    20101 cgcagttgtg gtggtgcatc cgccgccaga ggttccggta cgcgctgctc cagtacggca
    20161 acgcgtaagc cgctaccctg taagccgcag gcaaccgcca gcccgaccat gccgccgcca
    20221 acaatggcta catcaacact ttgcattgtt tattccttaa aaccgccttc aacgcgccac
    20281 ccaaccaaga gtgcgctgcg ccagcacatc gcgtgccggg gtaaataatt ccatcgtcat
    20341 cagcccgatg ttgcgcccga caaccagcgg cgtccaacgg ttggcaaaaa gatgtacaag
    20401 gctgtccgtg acgccgatgg ttgcttcgcg atcgctctgt cgacgctgct gataacggca
    20461 caatacgccg taatcaccca tgtcttctcc gcgttcctgc gcctgagtca gggtttccgc
    20521 aaggctcatg acatcacgca taccgaggtt aaatccttgc ccggcgatgg ggtgcagagt
    20581 ttgcgccgca ttgcccacca gcacggtacg atgggtaata ggtttggcgg cgcgggttag
    20641 cgctagcgga taagcactgc gtttaccggc atgagtaatt ttcccaagtc gccagccaaa
    20701 ggccgactgg agttcacggc aaaacttctc gtcgctccac gacagcacct cttcgcgccg
    20761 ttccagtgga tgacaccaga ccagcgaaca gcgtccgtca gacatcggca acatcgccag
    20821 cgggccatgt tgcgtaaagc gttcaaaagc gcgcccttca tgcgcaacgg aagtcgcaac
    20881 gttcgcaatc acagccagtt gttcgtaagg atcctgctgc cagtcaacgc cgcacacggt
    20941 ggctaacgct gaatgggtgc catcagcggc taccagcacg cggcccgtca gcgtctcacc
    21001 actctccagc gtcacttcaa cgtggctctg agtacgggca acgttagcca cgcgatcagg
    21061 gcaatgcagc gttacgccag gtgctttgcg cagcaatgca aacagccgtt gcccgacatt
    21121 gtgcaattcg acaacctgcc ccagcgccgc cagttggtaa tcttctgcgg caagggtgac
    21181 aaatccagcg tgaccacgat cgctgacatg cactgtggtg atggcagttg cgcaatcagc
    21241 cagagattgc cagacgccga tgcgcgccag ttgctgacag gtacccgccg ccagcgcaat
    21301 agctcgtcca tcaaagcccg gatgcgcatg tgactctggc gcggtcgctt caatcaaatg
    21361 tactgacagc gccccgtgac ttaaccggga aatagccagc gccagcgtcg cgcccgccat
    21421 gccgccaccg acgatgatta cactcattgc tttctcgcag ctgccatcaa cgcttcgatt
    21481 tcttccggct ttttcaccac gctggcggtg aggttttcat taccggtttc ggtaatcaca
    21541 atatcgtctt caatacgaat gccgataccg cgatactgtt ctggcacttc tgcatccggc
    21601 gcaatataca gccctggttc tacggtcagt accatacccg gttccagaat acgcgagcga
    21661 tcctgaccat aaacaccaac gtcatgaaca tccagcccta accagtggct aaggccatgc
    21721 ataaagaaag gacgatgggc gttctgagcg atcagttcat caacttcacc tttcaggatg
    21781 ccgagtttta ccagaccgct aaccatgatg cgcaccactt caccagtgac ttcctgaatg
    21841 gaagttcccg gacgatacag gcgcaggctg ttttcaagag actccagcac aatgtcatag
    21901 atttcacgct gagcctgagt gaatttgccg ttgaccggga aggtgcgggt aatgtcgcca
    21961 gcgtaacctt tgtattcaca acccgcgtca atcaacacca ggtcaccgtc acgcagttca
    22021 cactcgtttt cggtgtagtg caaaatgcag ccgttttcac cgctgccaac aatggtgtta
    22081 taggaagggt aacgcgcacc gtggcggtta aattcgtggt gaatttcgcc ttccagatgg
    22141 tactcgaaca tccccggacg gcatttttcc atcgcccggg tatgcgccag tgcggtgatt
    22201 tcacccgcgc ggcgaagtac ggcaatctct tctggtgatt tgaacaggcg catttcatga
    22261 acaacaggac gccagtcgat catcgttgcc ggtgcggtga gattttgccg cgaaccttta
    22321 cgcagtttct caagcgcgct gttcacgatt tcatcagcat atgcatgttc gccctgagcg
    22381 tggtaaacca catccagacc attaagtagt tgataaagtt gctgattgat ttcgctgaat
    22441 gccagtgcgc ggtcaacgcc cagtttttct ggcgcggcat cctggcctaa gcgacgacca
    22501 aaccagatct ctgccgtcag gtctcgaacg cggttaaaca aaacgctgtg gttatgagtg
    22561 tcatcgcttt taatcagcac cagcaccgct tccggttcgt taaagccggt gaagtaccag
    22621 aagtcactgt tctgacgata ggggtattcg ctgtcggcgc tacgtgttac ttctggtgca
    22681 gcaaaaatca gcgcggcgct gccgggttgc atttgctcta ccagggcctg acggcgacgc
    22741 tgaaattctt gccgggatat atcactcata acactctcct tacgtttttt gtttttagtg
    22801 tagagtcggt ttttgtactt ctggcgcggt cggttgcgga tgagtaaagg tgtcgtggca
    22861 taacagcgcg gcaacacgga cgtactcgat gatctcttca agcgacattt caagctcttc
    22921 ctgatcttcg tcttcgtcgt aacccaactg cgcgatgtta cgcagatcgt cgatggcttc
    22981 accggtttcg ccggtcactt tgtccagctt cggttgcgta acgccaagac caagcaggaa
    23041 gtgattgacc caaccagcca gcgcatcagc ccgatcgaaa acgctgacat catcgccatc
    23101 aggcagataa agctgaaaaa ggaagccgtc atcctgcagg gcatcgccgg tggcagagtg
    23161 cattttgcgc agtgcctgtg ccagctcatg accgaaagcc atgccttcgt tcgtcaggtc
    23221 gtgaattagc ggcagccatg agctgtcatc gttaccgcca catatcatcc cactgattaa
    23281 accatgcatc tcagcagggg tcagacccgt cccttgttgg ttcagatact ggttcatttc
    23341 gttgtaacca ggcatttcgt tctgtataga cataagcatt cgtcgtcaaa gggaggaata
    23401 ttcatgatat gctaccactt tgggccctgg tggaccagaa aagggcttgt ctcttctcat
    23461 cagggtagct atagtgtcgc cccttcgcag accatgggtc taaagacgaa ggcagcgcag
    23521 tcaatcagca ggaaggtggc atgtctgcac aacccgtcga tatccaaatt tttggccgtt
    23581 cactgcgtgt gaactgcccg cctgaccaaa gggatgcgtt gaatcaggca gcggacgatc
    23641 tgaaccaacg gttgcaagat ctgaaagaac gcactagagt cacaaatact gaacagttgg
    23701 tcttcattgc cgcattgaat atcagctatg agttagcgca agaaaaagcg aagactcgtg
    23761 actacgcggc aagtatggaa cagcgtattc ggatgctgca gcagaccata gaacaagcgt
    23821 tacttgaaca aggtcgcatc accgaaaaaa ctaaccaaaa ctttgaatga cacttttcgg
    23881 tttactgtgg tagagtaacc gtgaagacaa aatttctctg agatgttcgc aagcgggcca
    23941 gtcccctgag ccgatatttc ataccacaag aatgtggcgc tccgcggttg gtgagcatgc
    24001 tcggtccgtc cgagaagcct taaaactgcg acgacacatt caccttgaac caagggttca
    24061 agggttacag cctgcggcgg catctcggag attcccttct tatctggcac cagccatgac
    24121 gcaactacca gaacttccac tgacattatc ccgacaagaa atccgcaaaa tgattcggca
    24181 acgtcgtcgt gcgttaacgc cggaacaaca gcaggaaatg ggtcaacaag ccgctacccg
    24241 gatgatgact tatcccccgg tggtgatggc acatacggtc gctgtattcc tctcttttga
    24301 tggcgaactc gacactcagc cactcataga acaactctgg cgcgccggta agcgcgtata
    24361 tcttccagtt ttgcatccct ttagtgccgg taatttgctg ttcctgaatt accatccgca
    24421 aagcgaactg gtgatgaaca ggttgaagat ccatgagcca aaactggatg tgcgtgacgt
    24481 gctacccctt tcccgattag acgtgttgat cacaccgctg gtcgcctttg atgagtacgg
    24541 tcagcgcctg ggaatgggcg gtggttttta tgatcggacc ttacaaaact ggcagcacta
    24601 taaaatgcaa ccggtgggtt atgcgcatga ttgtcagttg gtggaaaaac tccccgttga
    24661 agagtgggat atccctcttc ctgcggtggt tacaccgtcg aaagtctggg agtggtgagg
    24721 gcgacacatc ccgcatcgcc ctgattgaca tcgttgattc tttaacctaa tttagtgagt
    24781 aagggtaagg gaggatgact cctcccctga gactgactgt taataagctg ggaaacttat
    24841 gagtaacagt acaatcagta tgatgacaag tcgcatcata acccttctcc ttcaagccct
    24901 cgcttcggtg agggctttac cgttacagcc ccatgctgcc ctgccatcgt aaatccccat
    24961 taaataaaca caacgcattg atctgacttt gatttatttt ctggagcaga ctcgcaaagt
    25021 agaatgcgca atgcggcaac ggtgtggaga agggataaaa aaacgggcaa gtcagtgacc
    25081 tgcccgttga ttttcagaga aggggattta gtacagcaga cgggcgcgaa tggtacccgg
    25141 aatagctttc attgcctgca gcgctttttc ggcaacgtct tcgtcggctt caatatcaat
    25201 aactacataa cccatctggg cggaagtttg tagatattgc gcggcgatgt tgacgccctg
    25261 ctcggcaaaa attttgttga gcgcagttag cacgcccgga cggttttcgt ggatgtgcat
    25321 cagacgacgc ccaccgtgca gtggcagcga gacttccggg aagttcaccg cagagagcgt
    25381 tgagccattg tcagaatact tgatcaattt acccgcaact tccaggccga tattctcctg
    25441 cgcttcctga gtcgaaccgc caatgtgtgg cgtcagaagg acgttgtcaa attcacacag
    25501 cggagaggta aatggatcgc tattggtcgc cggttcagtc gggaatacgt cgattgccgc
    25561 ccccgccaga tgtttgctcg ccagcgcgtc acacagcgct ggaatatcca ccacagtacc
    25621 gcgcgaagca ttaatcagca gcgagccggg tttcattagc gaaatctctt tcgcgcccat
    25681 catatttttg gtggacggat tctctggtac atgcagactc accacatcgc tcatattcag
    25741 caggtcagaa agatgctgta cctgagtggc gttgcccagc ggcagtttgt tttcaatatc
    25801 ataaaagtaa acatacattc ccagcgattc agccagaatg cccaattgcg taccaatatg
    25861 accgtagccg atgataccca gctttttgcc gcgcgcttca aaagaacccg ccgccagttt
    25921 gttccacacg ccacgatgcg ctttagcatt ggcttccggc acgccgcgca atagcagcag
    25981 cagttcgcca atcaccagct ccgcaacaga gcgcgtattt gagaacggtg cgttaaatac
    26041 cgggatcccg cgctttgccg ccgcatccag atcaacctgg tttgttccga tacagaaaca
    26101 gccaatagcg accagttttt ctgcggcgtt gatcacgtct tcagtcagat gggtacggga
    26161 tcgcaggccg atgaagtggg catcgcggat ggattctttt aattgttcat catccagcgc
    26221 gcctttgtga aattcgatgt tggtgtaacc agctgcacga aggctttcca gcgccttttg
    26281 gtgcacgcct tctaccagca gaaacttaat cttgtctttc tccagcgata cctttgccat
    26341 ttacccaatc ctgtcttttg aaatgttgtg tgcggatttg catccgcctt tcaacatatc
    26401 aaaaaataat attgcggcaa tatgaacgtt tgcgtcgcga tgttgaggaa atatcacgca
    26461 aaagtatttc ttggatgaat atgctggttt agaagattta tttagagcaa tcgacaattg
    26521 ccaggtaaaa acgtgacaca tgtcaccaaa tttaatgaag agaatttttt taacggggga
    26581 ggttcccccg tcagatcatt tcacaatagt tttgacaccg tcaggtgtgc caatcagcgc
    26641 aacgtccgcg ccacggttag caaacaaacc aacagtcacc acaccaggaa tcgcatttat
    26701 ggcgttttcc atcgctatcg ggtcaaggat ttccatgccg tggacgtcga ggatcacgtt
    26761 gccgttatcg gtcactacgc cctgacggta ttccggacga ccgcccagtt tcaccagctg
    26821 gcgcgccact gcactacgtg ccatcgggat aacttctact ggcagcggga atttgcccag
    26881 aatatcaacc tgcttggaag cgtctgcaat acagataaat ttttccgcaa ccgaagcaat
    26941 gattttttca cgggtcagcg ccgcgccgcc gcctttgatc atttgcatgt ggccgttgat
    27001 ttcatctgcg ccatcaacgt agataccaag gctgtcgact tcgttgagat caaaaacgtg
    27061 aatgccgagg cttttcagtt tttcagtgga agcatctgaa ctggaaacgg ccccttcaat
    27121 ctggcctttc attgtaccga gtgcgtcaat aaagtgtgcg gcggtggaac ctgtacctac
    27181 accaacaatg gtgccgggct gaacatactg aagtgccgcc catcctactg cttttttcaa
    27241 ttcatcctgc gtcatgatcg tttcgcctgt ggtatgaaat ttcacacgca ttatatacaa
    27301 aaaaagcgat tcagaccccg ttggcaagcc gcgtggttaa ctcatccata aaatatcgcg
    27361 caatggcggg catccccttt cgccccgcaa ataaagcata caacggtctg ggtatgccgc
    27421 tccacggtgc aaacaggcgc accagttccc cgttcgcaag cccctgttta caggcaaact
    27481 gaggcaataa cgccacgcca ttcaacacaa ccagggcgcg aacttgctga gcgaaatgca
    27541 ttagcgcaaa tccctgatct tttaagctgc gcgttaacca ctgatacaaa tgttacccgc
    27601 caacggcttc ggtatatgca acccgacaca aaattgtgtc atagtgcagg aaaaagcatt
    27661 taccaggagc agacaacagc aatgaaacgc ccggactaca gaacattaca ggcactggat
    27721 gcggtgatac gtgaacgagg atttgagcgc gcggcacaaa agctgtgcat tacacaatca
    27781 gccgtctcac agcgcattaa gcaactggaa aatatgttcg ggcagccgct gttagtgcgt
    27841 accgtaccgc cgcgcccgac ggaacaaggg caaaaactgt tggcgctact acgccaggtg
    27901 gagctgctgg aagaagagtg gctgggcgat gaacaaaccg gttcgactcc gctgctgctt
    27961 tcactggcgg tcaacgccga cagtctggcg acgtggttgc ttccggcact ggctcctgtg
    28021 ttggctgatt cgcccatccg cctcaacttg caggtagaag atgaaacccg cactcaggaa
    28081 cgtctgcgtc gcggcgaagt ggtcggcgca gtgagtattc aacatcaggc gctgccgagt
    28141 tgtcttgtcg ataaactcgg tgcgctcgac tatctgttcg tcagctcaaa accgtttgcc
    28201 gaaaaatatt tccctaacgg cgtaacgcgt tcggcattac tgaaagcgcc agtggtcgcg
    28261 tttgaccatc ttgacgatat gcaccaggcc tttttgcagc aaaatttcga tctgcctcct
    28321 ggcagcgtac cctgccatat cgttaattct tcggaagcgt tcgtgcaact tgctcgtcag
    28381 ggcaccacct gctgtatgat cccgcacctg caaatcgaga aagagctggc cagcggcgaa
    28441 ctgattgact taacgccagg gctattccaa cgccggatgc tctactggca ccgctttgct
    28501 cctgaaagcc gcatgatgcg taaagtcact gatgcgttgc tcgattatgg tcacaaagtt
    28561 cttcgtcagg attaatccat caaataatgc ctgatagcac atatcaggcg ttatcctcac
    28621 ttctttttgt attccttgaa tcacattaca aaatagacaa atctcaggcg gcaaaaaacg
    28681 acgtctgaat gcattttttt gctggcgaca aacccacgta aaaagctcac cgtaagcgca
    28741 aataccctca ctttgattgc gttttacgga gcaaataatg tctaacgtgc tggtgtggca
    28801 acagcttgcc aacaatgaat tgagccgtcg ggagaaaact gtcgactcgc tggttcagca
    28861 aaccgcggaa gggatcgcca tcaagccgct gtataccgaa gccgatctcg ataatctgga
    28921 ggtgacgggt acccttcctg gtttgccacc ctacgttcgt ggcccgcgtg ccactatgta
    28981 taccgcccaa ccgtggacca tccgtcagta tgctggtttc tcgacagcaa aagagtccaa
    29041 cgctttttat cgccgtaacc tggccgccgg gcaaaaaggt ctttccgttg cgtttgacct
    29101 tgccacccac cgaggctacg acttcgataa cccacgcgtg gcgggcgacg tcggcaaagc
    29161 gggcgtcgct atcgacaccg tggaagatat gaaagagctg ttcgaccaga tcccgctgga
    29221 taaaatgtcg gtttcgatga ccatgaatga cgcagtgcta ccagtactgg cgttttatat
    29281 cgtcgccgca gaagagcaag gtgttacacc cgataaactg accggcacca ttcagaacga
    29341 tatcctcaaa gagtatcttt gccgcaacac ctatatttac ccaccaaaac agtcaatgcg
    29401 tattatcgca gacatcatcg cctggtgttc cggcaacatg ccgcgattta ataacatcag
    29461 tatcagcggt taccacatgg gtgaagcggg tgccaactgc gtgcagcagg tagcatttac
    29521 gctcgccgat aggattgagt acatcaaagc agcaatctcc gccgggctga aaattgatga
    29581 cttcgcccct cgcctgtcgt ttttcttcgg cattggcatg gatctgttta tgaacgtcgc
    29641 catgttgggt gcggcacgtt atttatggag cgaagcggtc agtggatttg gcgcacagga
    29701 tccgtataac aacgttatcc gcaccaccat tgatcattgt gcacatccca tgtatcggga
    29761 ttatctgcat cgatatctgg aaaatgcgtc cggcggacat attcaccacg atcttagcca
    29821 cgtcttcgac ttacaccgta atttaattgc aaccggatcg atgctgggtt aatggtttgt
    29881 cccgtcacga tcttcagcaa tatgctgaag catcgtggcg gtataacgat gatttctgat
    29941 tgaataaaga tattcctgca tatacatcgg cctgtgcggc gcatcaaaat atttcagcgt
    30001 tgccggattc accagtcgcc aggcaaaatg cgggatcacc gtcagaaaat gtccccgctc
    30061 cacggcgctg attttagcca taaaactgta gggacgataa ataatggtgg gattgatccc
    30121 acaaggacgc atattcgcat caatcattgc ttcgaaattg gcccgattct gaaagcgcat
    30181 ttgtagccag ggcagctcct gaagcagttc ttgctcttgt cgctcttcat aacggcgaga
    30241 aacgagaaag cccaatcgca gcggcggtag ttcactgata gtcaggtttt ccagttcctg
    30301 aactcgtgca gatacatgct gtggggagat aataaaatct aactgacggt caaaaagatt
    30361 atcgataacg ccattttcgc tgaactcaac tggctgggcg gtcacgcctt cgtacttatc
    30421 tccgagacta atcaactgat caaaaataat tgtcggataa gtattatcta tgcctatcac
    30481 aatttctcgt taacgacgtc ctgagttatg gatttcatta tcaatcgcag aaagtcgttg
    30541 ataaaccggg aacagttttt gatacagttc ctgtccggct ttattgaggc tgatattatt
    30601 atctttgcgg gtaaatagcg tatagccaat ttgttcttcc agcgcggcaa tgcttttgcc
    30661 aaatcgcgaa gccgtcatat ggattttttc tgccgcccgg gcaatattat tggtttgcgc
    30721 taataaaatg aaattgcgca tctttttcga aataaaaatg tccatctccc ctctcccgca
    30781 tccttgtccg cttaaccgtt tcagtcagcc tatccttgat gaaaccgcga gcaaagatag
    30841 gtgattacgt catggtttta cagaaaaagg aggcaatatc gggtaaaggc attagcccga
    30901 cgaatacgtc gggctacaag tattaatgtg cagcaggtgt tttagcgggt ggttgatcca
    30961 caggttctaa ctggaagact acatcgacct gatcatcaaa ctgaatagcg gcctgctcgt
    31021 aagtttcctg tgcggacacc ggtgcagcat cagctttcat catccgcacc attgggctgg
    31081 gctgatagtt ggaaacatga taacgcacgc tatataccgg cccgagtttg cgattaaagc
    31141 cattcgccag ttcctgcgcc tgatgaatcg cgttatcaat cgctgcctta cgcgctttgt
    31201 ctttataggc atccggctgc gccacgccca gcgacacaga acgaatttcg ttaagacccg
    31261 cctttagcgc gccgtccagc aaggaattca gtttgtctaa ctgacggagc gtgacttcca
    31321 ccgttctcac tgcgcggtag cctttcagga tacttttacc atcctgataa tcataatctg
    31381 gctgagtgcg taagttcgct gagctgatat cttttttcgc gatctgattg agctcaagga
    31441 aggaaatgta ttgtgcgacg cgctcatctg cctgtttctt ggcagtagcg gcatccttcg
    31501 cggccacgtt aacttcaatc gcaagagtgg caatgtctgg caccgcatcc acgcttgccg
    31561 taccggaggt gacaatatgc ggtccatccg gcaattcgtt agcctgcgct gccatcccgc
    31621 taatacccat taatgccgcc agggcgataa ctttgaactt cacgttagtc tctccgtgtt
    31681 aattgacttg cccaaaattc aggacctctg gaggcaagct tagcgcctct gttttatttt
    31741 tccatcagat agcgcctaac tgaacaaggc ttgtgcatga gcaataccgt ctctcgccag
    31801 ctgcaaggca ataaaccaca taacacatcc cactaccaga ttgataatgc gctgtgattt
    31861 tgccgtgcgc aggcgcggtg ccagccaggc tgcgagaata gccagaccaa agaaccacag
    31921 gaaagaggcg ctaattgttc cgagcgcaaa ccaacgtttt ggttccacat caagttgccc
    31981 gccaaggctg cccagcacaa caaaagtatc caggtaaaca tgcggattca gccaggtcac
    32041 tgccaacatg gtggcgataa ttttccatct gccttgtttc agaacttcgg cactggctaa
    32101 ctcaatgtta ctgctcattg ctgttttaaa agcgccaaaa ccataccaca gcaagaaggc
    32161 tacgccgccc caggtgacca gcgccagcaa ccacggcgac tgcatcaata acgcgctgcc
    32221 accaaaaatc cccgcacaaa tcaggaccaa atcgctgata gcacaaagta aggcaatcat
    32281 aatgtggtac tgacgacgaa tgccctgatt catcacaaaa gcattttgtg gaccgagcgg
    32341 taggatcata gccgccccaa gtgcaagacc ttgaaagtaa taagaaaaca cgttggatat
    32401 tccgaattaa gtatctgatg cagagactat atcgcgggat tgttattagc ggaaattgat
    32461 aatattaatt ggcaatcaga aaaactaata agagagaggc cagagagtat tatctggcct
    32521 cagcgttgat tacgcagctt tgtcttcttt cacccgctta aagttcacat ccatttgcgg
    32581 gtacgggaag ctgataccgg cggcatcaaa ttcacgttta atacgctcca gcacatccca
    32641 gtacacgttt tgcagatcgc cgctgttgct ccagacgcgg accacgaaat taatcgacga
    32701 tgcgccaagt tcgttcagac gcacagtcat ttcgcgatct ttcaaaatgc gatcttcaga
    32761 ctggataata tcggtcagga tctgcttaac ctgatcgata tcggaatcat acgccacgcc
    32821 aataataaat tcgttacggc gagctggctc gcgggagaag ttaataatat tcccggcaat
    32881 aattttaccg ttcggaataa cgataatttt accgtctgca gtacgcatgg tggtggagaa
    32941 aatctgcaca ctcagcacag taccggctac gccgccgagg tcaacatatt ctccggcacg
    33001 gaacgggcgg aacatgacaa gtaacacgcc agcggccagg ttagacagtg acccctgcaa
    33061 agccagacca acagctaagc ctgcggcacc gagtacagca atgactgacg ctgtttgtac
    33121 gcccacgcgc cccagtgcag cgattagcgt aaaggcgata attccgtaac ggactaatgc
    33181 agaaagaaaa tcagcaacag tggcatcgat tttacgggag atcatcaggc gattcaccgc
    33241 gttggaaatc atccgcgcga taatcaaacc aacgatgatg atcgcgagtg ccgccacgat
    33301 gtttactgca taacttagca gcagcgcctg gttagccacc agccagcttc ccgcgccgtt
    33361 tatgctatcg acaacattca aatcttccat tcaatattcc ttttcaactg actcaaaatg
    33421 gagaaataca ctccgccgcc ttatgacgga cagtctgaca agggtaaaca ataaagcata
    33481 attttgccaa atagatcaca gatttctcat ctgtatcacc ttgcttttgg cgaaaaaaag
    33541 acccgcagag cgggccttga gataagcaga aaggaatatc ttacagaacg tcgatcgcgt
    33601 tcagttcctg gaatgctttt tccagacgag cgatcatcga agtctgaccg gcacgcagcc
    33661 atacgcgcgg atcgtagtat ttcttgttcg gctgatcttc gcctttcggg ttgcccagct
    33721 ggccttgcag gtaagcttcg ttttctttgt agtagttcag aacaccttcc caggttgccc
    33781 attgggtatc ggtatcgatg ttcattttca ctacgccgta gcttacggag tctttgattt
    33841 cctgagcagt agaaccggaa ccaccgtgga atacgaagtt caggctgttg tgcggcaggt
    33901 tgtgtttctt agaaacatat tcctgagagt cgcgcaggat ggtcggagtc agaacaacgt
    33961 tacccggctt gtaaacaccg tgtacgttac cgaaggacgc agcgatggtg aaacgcgggc
    34021 tgattttgct cagctcggtg tatgcgtaat caacgtcttc cggctgggtg tacagtgcag
    34081 aagcgtccat gtggctgttg tccacgccgt cttcttcacc accagtgcaa cccagttcga
    34141 tttccagagt catgccgatt ttggacatgc gctccaggta tttagagcag atttcgatgt
    34201 tctcttgcag agattcttca gacaggtcga tcatgtgaga agagaacagc ggcttaccgg
    34261 tagcagcgaa gtgtttttca cccgcgtcca gcagaccgtc gatccacggc agcagtttct
    34321 tcgcgcagtg gtcagtgtgc aggataaccg gaacaccata atgttcagcc atctgatgaa
    34381 cgtgatgcgc accagagatc gcacccagga tagcagcgcc ctgcggaacg tcagatttca
    34441 cgcctttacc agcgataaag gaagcaccac cgttggagaa ctgaacgata accggcgctt
    34501 taactttagc agcggtttcc agtacggcgt tgatggagtc agtaccgacg cagtttactg
    34561 ctggcagtgc gaagttgttt tcttttgcta cctggaaaac tttctgtacg tcatcaccag
    34621 tgattacgcc aggttttacg aaatcaaaaa tcttagacat gtctcttgtc ctgtatcgtc
    34681 gggccttata ctcgtcatac ttcaagttgc atgtgctgcg tctgcgttcg ctcaccccaa
    34741 tcacttactt atgtaagctc ctggggattc actctcttgt cgccttcctg caactcgaat
    34801 tatttagagt atgaaaaagt gcgtgctcta aaagcgcgct gaaacaaggg caggtttccc
    34861 tgccctgtga ttttttactt cttagcgcgc tcttcgagca tcgctactgc aggcagtact
    34921 ttaccttcta cgaattcgag gaatgcgccg ccgccagtgg agatgtagga gattttgtca
    34981 gcaatgccga acaggtcgat tgctgccaga gtgtcgccgc cgccagcgat ggagaacgct
    35041 tcgctgtctg cgatagcgtt agccacgatt tcagtacctt tgcggaagtt cgggaattcg
    35101 aacacgccaa ccggaccgtt ccacagaatg gttttcgcat tcttcaggat ttcagccagt
    35161 tcctgagcgg aagcatcacc gatatccagg atctgctcgt cagctttcac atcgttaaca
    35221 gatttcaggg tagccggtgc agtttcagag aactcggttg ctacgcgaac atcagacgga
    35281 accgggatgt tgcaggtggt cagcagacgt ttagcttcgt caaccaggtc agcttcgtac
    35341 agggatttac ccacatcgtg gccttgtgcc gcgataaagg tgttagcgat accaccgcca
    35401 acgatcagct ggtcagcgat tttagacagg gagtccagaa cggtcagttt ggtagatact
    35461 ttagaaccac caacgatagc caccatcggg cgagcaggtt ctttcagtgc tttacccagc
    35521 gcgtccagtt cagctgccag cagcgggcct gcgcacgcaa catcagcgaa tttaccgata
    35581 ccgtgagtag aagcctgcgc gcggtgagca gtaccgaatg cgtccattac gaacacgtca
    35641 cacagtgcag cgtatttttt ggacagggtt tcgtcgtctt tcttctcgcc tttgttgaag
    35701 cgaacgtttt ccagaacaac cagttcacct tcagcaacgt caacgccgtc gaggtaatct
    35761 ttaaccagac gaaccgggtt agacagtttg tctttcaggt agttaacaac cggcagcaga
    35821 gagaattctt cgttgtactc gccttcggta ggacgaccca ggtgggaagt taccatcact
    35881 tttgcgcctt gtttcagggc cagttcgatg gtcggcagag aagcacggat acgcgcgtcg
    35941 ctggttactt tcccatcttt tactggtacg ttcagatccg cacggataaa tacacgtttc
    36001 ccagcaagat ccagatcggt catcttaatt acagacatgg tgaatcctct cgttgattct
    36061 aaaagttttg cagacgctgc ttgcgtctta cctgaaagca acagtagcca tagctaacgt
    36121 cgtgtcgagc attcggttag caaagcccca ttcgttatcg caccagacca acgttttgat
    36181 caggtgtgcg ccactgaccc gggtttgagt gccatcgaca atggcactgt gcggatcgtg
    36241 gttaaaatct acagagacca acggcaattc cgtatagtca actataccat gaaatgcacc
    36301 ttgtgctgct ttttgcagca acaggttgac ttcattggct tttacaggtt tcttcaccgt
    36361 cacgcttaaa tcgattgccg tcacatttat ggttggcaca cgtaccgcaa tcgcctcaaa
    36421 gcgatcgtta aattgcggaa aaaatcgtgt gataccggcg gccagtttag tatcgaccgg
    36481 aatgatcgac tgactggctg cccgagtgcg acgcaggtca ggatggtatg catcaataac
    36541 ctgttgatcg tgcatggcgg agtgaattgt ggtcacagtg ccggactcaa taccgtacgc
    36601 atcatctaac aatttgatga cgggaattat gcaattcgtg gtacaggaag cgttagaaac
    36661 gatgcggtgt tccgcacgaa gttgatcctg attgacgcca taaacaacgg tcgcgtcgag
    36721 atcgttactg ccaggatgtg aaaagagcac ttttttagcc ccggcggcaa tgtgtgcttc
    36781 accatgctcg cgggagccat atacgccggt gcagtcgagg actacatcaa cgccaagttc
    36841 acgccagggg agcgattgca gtgaacgttc atgcaataca cggatggcgt catcaccaac
    36901 aaaaagttga tcgcgttcct gtcgtacttc ccatgcaaaa cggccatggc tggtgtcata
    36961 tttcaacaaa tgcgccatac ccgcagcatc cgccagttcg ttgattgcca ccacggtaat
    37021 ttccgcacgg cgtccggatt catacaaagc acgaaccaca ttacgcccga tgcgaccgaa
    37081 gccatttatc gctacgcgta cggtcataga tctcctgcaa ggttttccct gagcaaattt
    37141 gccagacaga gtaatccagc aaatcgttcg gggaaacctt acctgtcgca aactgcgact
    37201 gattggttaa ttgtcgaaca tttaatcgac tgaaacgctt cagctaggat aagcgaaacg
    37261 tggaataaaa ggaatgattg tccagtcaaa gaagacaatt atctgactta cgtcacgttt
    37321 tcagccccgc ccctgtggaa tttattccac tttgcagaat cacgaataag cgtagcaaaa
    37381 tcttttcgtt gtgccgtcac aaaccctgac ttatatcaga atatgactga tgcagcgtcg
    37441 atattatccg caatacgtcc gagtttcgga gtactccaat gatgacattg ccctacccat
    37501 attcttcctc cgcacgtttt tacgcgctgc gcctgctccc cgggcaggaa gttctctccc
    37561 aattacgcgc cttcgcgcag caacaacaac tccacgccgc gtggattgca ggttgtaccg
    37621 gcagtctgac tgacgtcgcc ttacgctatg ccgggcaaga aaacactgcc ctgctaagcg
    37681 gtaagttcga agtcatcgca ttgaacggca cgctggaaca gagtggtgaa catcttcatc
    37741 tctgcgtttc cgatccacac ggcacgatgc ttggcggtca tatgatgccc ggctgtaccg
    37801 tgcggaccac gcttgaactg gtgattggct gcctggagga actggcgttt agccgccagc
    37861 tttgcgcgct ttctggttac gacgagttgc atatttcccc cgttaaataa ctattttcag
    37921 agaggttttc ttatggcgcg tcgccacttt tccagtcagg cactggtgtt gatcgttatc
    37981 tctatcgcca tcaatatgat cggcggacaa ctggccagca tggttaaatt acctattttt
    38041 ctcgactcca tcggcacact tattagcgcg gtactgcttg gtccggtgat cgggatgctg
    38101 accggtttac tgaccaattt actctgggga ttgctgaccg acccgatcgc cgccgcattt
    38161 gcgcccgtcg cgatggtcat cggcctggtc gccggttggc tggcgcgagc gggttggttt
    38221 cgcaccctgc caaaagtcgt tgttagcggc gtgattatta cgcttgcggt aacggtcgtc
    38281 gctgtaccgc tgcgtactgc gctgttcggc ggcgtgacag gaagcggagc cgatttgttc
    38341 gttgcctgga tgcactcgat ggggcaaaac ctggtggagt cggtggcgat tacggtgata
    38401 ggcgcgaacc tggtcgataa aattctcaca gcggtgattg tctggctttt actgcgtcag
    38461 ctgccgattc gcaccacgcg ccattttccg gcaatggctg ccgtgcgcta aatgcatccg
    38521 tttacatcct taacgctgtg ggcgctggcg gcctgcacca cgcttatcct gcccacacag
    38581 acaatattgc cagtctatag cgcggcgacc tttttctgcc ttattgcgct aaaggccacg
    38641 cggcggcggg caaaatatgt cgtctggctg atgttctcgc tgggggctgg attgtggctg
    38701 gtgcacggtg gctggctgac agaatggcta agcggcacgc cacgctctcc agaacggtgg
    38761 gcgcatgcta ttacgctctg gttgcggatt ctggcgattg tttcaacctc acagttgtgg
    38821 atgcagtacg tccccgtgca acgttttatc cgcgctttgt ttgcttctcg cctgccgccg
    38881 ggcgtcgctt atctgtttgc cgggccgtta ttggtcgttg agcagttaaa acggcaattg
    38941 gcgattattc atgaagcgca gcgcgcccgc ggcgtgccgc tggatgaggg ctggtatcag
    39001 cgtttacgtg cgatgcccgc gttaattatt ccgctaacac acaatgcgct aaacgatctt
    39061 gctgtccgtg gtgcggcgct ggatatgcgg gcatttcgaa tcaacaatag gcgcactacg
    39121 ttatgggcac cagccgatag caccctacaa cgcgtagcac gttacaccat gatattgcta
    39181 atgctggctg aattcggagc gtggatatgg ttacgttaga acagtttcgc tattgcccga
    39241 cacactcaac gcatcctccg ttctgttatg actttcacta tgtcaaaccg gggatggtcg
    39301 ccatctttgg tgacaacggc agtggcaaaa gtacgctggc gcagctgatg gcgggctggt
    39361 atccggactt tctaccagga gagataaccg gcacggggac gcttctcggt acgcccattg
    39421 ggcgtctccc gcttaatgaa caatccgcca ccatccagct cgtccagcaa tcgccctatt
    39481 tgcagctctc cggctgtacc tttagcgtgg aagaagaagt tgcctttggg ccagaaaatc
    39541 tgtgtcttgc tgaaaaagag attatggcgc ggattgacgc cgccctggcc ctgaccgagt
    39601 gccagccgtt gcgccatcgt cacccggcaa cactctccgg tggagagaca cagcgtgtgg
    39661 tcatcgcctg cgccatcgcg atgcagccaa agctcttaat tcttgatgaa gcctttagcc
    39721 gcctgacgcc gaaggccagc gaaatgctgt tgcagcgctt acaacactgg gcgcttgaac
    39781 gtggttcgct gattattctt tttgaacgtc atcacacacc tttcctgaac cactgccagc
    39841 aagcgtggca attgcaaaac ggagcgctcc agccactatg ttaacgttaa atcagatatc
    39901 ttatcgctgg cctggcgcgg caacggattg tctttgcgac atttctctgc aactcaaaca
    39961 aggtgaatgg ctggcgctga ccggcgacaa tggcgcggga aaatcgacgc tgttacgagt
    40021 aatggcaggt cttcttacgc ccacagccga tacggttatg ctgcaacaac aagcgatgaa
    40081 aaacctgaaa aaccgccagc gcgcggcaaa gattggcgta ctgtttcagg aagcggaaaa
    40141 tcagcttttt cacagcaccg ttgctgacga aatcgcattt ggtttgatac tgcaaaaatg
    40201 cccggcggat gaaatcactc aacgtaccaa tgctgccctg caatgctgtc agttagcaga
    40261 cactgccagc gcgcatccgt tagatctgca tagtgcgcag cgacggatgg tcgccgtcgc
    40321 ctgcctggaa gcactctctc cgccactatt gctactggac gagccgagtc gggattttga
    40381 cgaaaattgg ttgagcgtgt ttgaaagctg gctggaaaag tgccgccagc gaggcacgag
    40441 cgtcgtagca atcagtcatg acgccgcatt tacccggcgt catttctctc gtgtggtgcg
    40501 actggaagat ggtgtaatca ggaacgttaa tccatcagac gatattcacc cgtagcggtc
    40561 atcattaagg tcaaatttgc aggcagactc tcttccagca cccggcgaac attcggcccg
    40621 tcggtacggt cataaaaggc ttctgcgtct gccagcgata accctcccgc cagcttgcgc
    40681 cccaccagac gctcccgcaa cgcagatgcg ggagctttga taaagatcga gaaatcacaa
    40741 aattgcgcaa gctggcacca cttctcgtcg tccaacaaca accagtttcc ttcgacgatg
    40801 acgagtggtg cggtaacgtg caacacatct tcaacaggat catgcttttg tcgatcgtac
    40861 tgcggccacg tacaatcccc ttccacaacc cggcacagat tttccgccag tttcgcaacg
    40921 ttgaatgtct ctggtgcgcc tttgaagggt cgcaattgat gcacatccag ccagctattg
    40981 taatggtgaa aaccatccat cggcagcgtc tgaatagcgg gcagttccgg gtcttgttgc
    41041 gcgagatatt cccagaaggt ggttagggta gatttacccg tccccggcgg agcgcaaaga
    41101 aaaaccaccg tacgccgctg cggatttact gtctgtaagg ccgccagcat acgcagcaat
    41161 ggtttatgta cattttcaat ttcatcatca gagtactgcg cctgtacctt tagtccattc
    41221 accgtcagtt cgattttcac ggcgttaatt cctttaaggt ggtcgttact gccagcgtca
    41281 gcgccgtttt gaccatcccg ctaaaacgcg cctctgacca gacggcgaaa tcggcgaact
    41341 tcatcgcttt caacgcgtca tagagcgtcg cattgcgggt tatgctattg acgttgctga
    41401 taaaatccca ggttatgtca tcagcaaatc tgggcgtttc atcctgttcg ttcagataat
    41461 gccaaaactg ggaaaaatgc gtgatatcgg cgtacagcca tttatccatt ttccccagcg
    41521 cataaatcaa acgcagcgca acatcaatat cgtcaagcgg gccgctttgg gcgagtaacg
    41581 gcttcactgc atattcgacg atctcttcat cgttatcgac aaacagcgac ggcagaaact
    41641 ggcgtatccc ctggagcaaa atagaatgcg caattttcat aaatgaaaat aaattgtcct
    41701 gtgcattcag ttgctcaagc acatcatctt ctgtcagcgt cgccattggt cttccgatag
    41761 atcaccatca atgcagggag tctattatat tacacgtttg gtccgcgccg ccgatcagta
    41821 atgtctgcgt ccgcacccca ttcgccgtct gttggatacc gttcaccaac tcgccgcccg
    41881 tcacgcccgt agcgctgaag aggatatcgt tccccctcgc cgataaccac tcgcccacga
    41941 aaagcaacgt cgtttaatgc ctggcgcatc gcggtgaccg caaggccatc aattttattt
    42001 ttgtcgccac agccggtttg cggccaggca gccaacgctg cctgttccgt aacgcgaaat
    42061 aatggccacg ccagggacat cattgcgcgg cctccccaat atcaacgcca aattgtgcca
    42121 gcagatattt ctcggcctgt tcattccaga taccgtgcgt tttctcaacc agccacgcca
    42181 gctctttaaa cagcggatcg gttttgcctt tttcggcaaa atcggcaatg gcggtcagcg
    42241 gcatggtcac gccgttataa atgagttttt taccgccagg gatatccggc agattgagca
    42301 cggtttctgg caccgcatcc aggccaccga tatgggtcac cataaacgac ggctgtaact
    42361 gccccgtggc gctaagggca atcgcctctt tcatgtcgtc cgtggaaccg ccggacgtgc
    42421 cgacaacgtg cgtactgttg taatggacgt tgtagaaatt aaacggcact ttgaagtttt
    42481 tatccgtcgg accggcaaag aagttcaggc agccatcttc cgccagtaat tcatccgcca
    42541 tctcgacgac tgaaggcacc gctgcataga caaatacgtc atcaaatccg gcatcatccg
    42601 tcaacgcccg cagcgtctgg accggatcgc tcatcccttt agtattcata tacaccagct
    42661 caatgccttt actggccgcc agttccaccg gcagcagcat ctgcacctgc gccagccgtt
    42721 tgtcgtcgat atcgactacc accacccgcg acggttgtat gccgccgtta atggcgtaat
    42781 caatagcgcc aattcccatc gggcctgcac acgccagtag cgcaatattg ccgccaggtt
    42841 tgacgcccat acgatgctca taaacatatt gcgtggtgtg ataattggca tgataagcgc
    42901 caataatgca gcacataggc tctgccagcg acgccgcagc aaaataagag ccgtgatagg
    42961 gtaatacgca gcccaaatta atggcgattt cgggaataat catataggtg gcgttaccgc
    43021 caaaatattc gtagctgtag cccgctgaat acccgcctgg taaccccatc gccggttgca
    43081 atacaaaacg ctggcctttt ttatatttgc cagtgagatt tttacccact tcgacaataa
    43141 ccccggcaca ttcatgcccg gtaatgaccg gatgattttc taaatcgtcg gggacgcgtt
    43201 tatgttcact accgagtaac gccgctttcc aggtggataa acaaacgctg tcagaaatta
    43261 cattcactaa cagttcatta tcggtaattt ctggcagctc aaattcgcgc agacggacat
    43321 cccgcttgcc ataaatagca gcaactttgg ttttcatttt tacctctgta atcagtgttt
    43381 atgttcagcg gttaattgat taaataaagt gtcgagtttt ggatcgccaa tatagttatt
    43441 aatcaatatc agtggtttat ccgtcacacg tttcacgcgc ccttccagac tggcgtgagt
    43501 aacgacgata tccgcatccg caggcacgtt ttcggtaatg ctg
//
LOCUS       DAZGJT010000030        43014 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZGJT010000030 DAZGJT010000000
VERSION     DAZGJT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43014
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.030"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..693
                     /locus_tag="ACHKFK_001800"
     CDS             <1..693
                     /locus_tag="ACHKFK_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO9052153.1"
                     /translation="LNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDDA
                     VRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQI
                     GGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPQKQLT
                     LLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGMA
                     KRIARLAEQSTD"
     gene            792..1805
                     /gene="ybjS"
                     /locus_tag="ACHKFK_001801"
     CDS             792..1805
                     /gene="ybjS"
                     /locus_tag="ACHKFK_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9052154.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPSLTHYGVSKLNFDFTLDITRAQEELGYQPVLTLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(1802..2632)
                     /gene="amiD"
                     /locus_tag="ACHKFK_001802"
     CDS             complement(1802..2632)
                     /gene="amiD"
                     /locus_tag="ACHKFK_001802"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO9052155.1"
                     /translation="MRRVFWLVAAALLLAGCTGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVETASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(2629..2952)
                     /gene="ybjQ"
                     /locus_tag="ACHKFK_001803"
     CDS             complement(2629..2952)
                     /gene="ybjQ"
                     /locus_tag="ACHKFK_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO9052156.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            3078..3593
                     /gene="ybjP"
                     /locus_tag="ACHKFK_001804"
     CDS             3078..3593
                     /gene="ybjP"
                     /locus_tag="ACHKFK_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9052157.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3811..4539
                     /gene="artP"
                     /locus_tag="ACHKFK_001805"
     CDS             3811..4539
                     /gene="artP"
                     /locus_tag="ACHKFK_001805"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO9052158.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            4557..5288
                     /gene="artI"
                     /locus_tag="ACHKFK_001806"
     CDS             4557..5288
                     /gene="artI"
                     /locus_tag="ACHKFK_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO9052159.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            5295..6011
                     /gene="artQ"
                     /locus_tag="ACHKFK_001807"
     CDS             5295..6011
                     /gene="artQ"
                     /locus_tag="ACHKFK_001807"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO9052160.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            6011..6679
                     /gene="artM"
                     /locus_tag="ACHKFK_001808"
     CDS             6011..6679
                     /gene="artM"
                     /locus_tag="ACHKFK_001808"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO9052161.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            6905..7636
                     /gene="artJ"
                     /locus_tag="ACHKFK_001809"
     CDS             6905..7636
                     /gene="artJ"
                     /locus_tag="ACHKFK_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO9052162.1"
                     /translation="MKKLVLAALLASFTFGASAAGKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(7665..8792)
                     /gene="rlmC"
                     /locus_tag="ACHKFK_001810"
     CDS             complement(7665..8792)
                     /gene="rlmC"
                     /locus_tag="ACHKFK_001810"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO9052163.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKAADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWIRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(8833..9321)
                     /gene="ybjO"
                     /locus_tag="ACHKFK_001811"
     CDS             complement(8833..9321)
                     /gene="ybjO"
                     /locus_tag="ACHKFK_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO9052164.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            complement(9381..10226)
                     /gene="potI"
                     /locus_tag="ACHKFK_001812"
     CDS             complement(9381..10226)
                     /gene="potI"
                     /locus_tag="ACHKFK_001812"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO9052165.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDSSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            complement(10223..11176)
                     /gene="potH"
                     /locus_tag="ACHKFK_001813"
     CDS             complement(10223..11176)
                     /gene="potH"
                     /locus_tag="ACHKFK_001813"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO9052166.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGIIDQPLTILHTNFAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIMPIMWFHKHQQKSVGEHG"
     gene            complement(11186..12319)
                     /gene="potG"
                     /locus_tag="ACHKFK_001814"
     CDS             complement(11186..12319)
                     /gene="potG"
                     /locus_tag="ACHKFK_001814"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO9052167.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLYSPGLV
                     HPLKVDVDASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            complement(12414..13526)
                     /gene="potF"
                     /locus_tag="ACHKFK_001815"
     CDS             complement(12414..13526)
                     /gene="potF"
                     /locus_tag="ACHKFK_001815"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO9052168.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLSLKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKEATPLVSAEVRDNPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            complement(13876..14352)
                     /gene="ybjN"
                     /locus_tag="ACHKFK_001816"
     CDS             complement(13876..14352)
                     /gene="ybjN"
                     /locus_tag="ACHKFK_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO9052169.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDMIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            complement(14440..15342)
                     /gene="rimK"
                     /locus_tag="ACHKFK_001817"
     CDS             complement(14440..15342)
                     /gene="rimK"
                     /locus_tag="ACHKFK_001817"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO9052170.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            complement(15403..16125)
                     /gene="nfsA"
                     /locus_tag="ACHKFK_001818"
     CDS             complement(15403..16125)
                     /gene="nfsA"
                     /locus_tag="ACHKFK_001818"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO9052171.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLCNNIEAVTELLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLNKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            complement(16109..16396)
                     /gene="ybjC"
                     /locus_tag="ACHKFK_001819"
     CDS             complement(16109..16396)
                     /gene="ybjC"
                     /locus_tag="ACHKFK_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFO9052172.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMIPAAVVVILQVAKRLAPQLMNHPPQYSRSEREKDNDANH"
     gene            16556..16813
                     /gene="grxA"
                     /locus_tag="ACHKFK_001820"
     CDS             16556..16813
                     /gene="grxA"
                     /locus_tag="ACHKFK_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO9052173.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            complement(16843..17220)
                     /gene="ybjM"
                     /locus_tag="ACHKFK_001821"
     CDS             complement(16843..17220)
                     /gene="ybjM"
                     /locus_tag="ACHKFK_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO9052174.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSHRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            17490..19175
                     /gene="ybjL"
                     /locus_tag="ACHKFK_001822"
     CDS             17490..19175
                     /gene="ybjL"
                     /locus_tag="ACHKFK_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO9052175.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            complement(19351..19887)
                     /gene="rcdA"
                     /locus_tag="ACHKFK_001823"
     CDS             complement(19351..19887)
                     /gene="rcdA"
                     /locus_tag="ACHKFK_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO9052176.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSRFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERIAG"
     gene            19971..21179
                     /gene="ybjJ"
                     /locus_tag="ACHKFK_001824"
     CDS             19971..21179
                     /gene="ybjJ"
                     /locus_tag="ACHKFK_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052177.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            21179..21994
                     /gene="ybjI"
                     /locus_tag="ACHKFK_001825"
     CDS             21179..21994
                     /gene="ybjI"
                     /locus_tag="ACHKFK_001825"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO9052178.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFNQ"
     gene            22079..22363
                     /gene="ybjH"
                     /locus_tag="ACHKFK_001826"
     CDS             22079..22363
                     /gene="ybjH"
                     /locus_tag="ACHKFK_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO9052179.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(22404..23636)
                     /gene="mdfA"
                     /locus_tag="ACHKFK_001827"
     CDS             complement(22404..23636)
                     /gene="mdfA"
                     /locus_tag="ACHKFK_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO9052180.1"
                     /translation="MQNKLASGVRLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            23921..24517
                     /gene="ybjG"
                     /locus_tag="ACHKFK_001828"
     CDS             23921..24517
                     /gene="ybjG"
                     /locus_tag="ACHKFK_001828"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO9052181.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            24575..25333
                     /gene="deoR"
                     /locus_tag="ACHKFK_001829"
     CDS             24575..25333
                     /gene="deoR"
                     /locus_tag="ACHKFK_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO9052182.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(25380..26582)
                     /gene="dacC"
                     /locus_tag="ACHKFK_001830"
     CDS             complement(25380..26582)
                     /gene="dacC"
                     /locus_tag="ACHKFK_001830"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO9052183.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            26829..27455
                     /gene="gstB"
                     /locus_tag="ACHKFK_001831"
     CDS             26829..27455
                     /gene="gstB"
                     /locus_tag="ACHKFK_001831"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO9052184.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(27452..28567)
                     /gene="yliI"
                     /locus_tag="ACHKFK_001832"
     CDS             complement(27452..28567)
                     /gene="yliI"
                     /locus_tag="ACHKFK_001832"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO9052185.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRMVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDLGEIPDDNPFIKESGVRAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(28678..29061)
                     /gene="bssR"
                     /locus_tag="ACHKFK_001833"
     CDS             complement(28678..29061)
                     /gene="bssR"
                     /locus_tag="ACHKFK_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO9052186.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            29274..30599
                     /gene="rimO"
                     /locus_tag="ACHKFK_001834"
     CDS             29274..30599
                     /gene="rimO"
                     /locus_tag="ACHKFK_001834"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO9052187.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWRKICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(30646..31974)
                     /gene="dgcI"
                     /locus_tag="ACHKFK_001835"
     CDS             complement(30646..31974)
                     /gene="dgcI"
                     /locus_tag="ACHKFK_001835"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9052188.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYGLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHIKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKSVTGDEYNAKNAIFTVSEAMEHFSYLPTGLYVF
                     AYKKDVYFQVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(31982..34324)
                     /gene="pdeI"
                     /locus_tag="ACHKFK_001836"
     CDS             complement(31982..34324)
                     /gene="pdeI"
                     /locus_tag="ACHKFK_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9052189.1"
                     /translation="MLSLYEKIRLIILFLLAALSFIGLFFIINYQLVSERAVKRADSR
                     FELIQKNVGYFFKDIERSALTLKDSLYLLKNTEEIQRAVILKMEMMPFLDSVGLVLDD
                     NKYYLFSRRANDKIVVYHQEQVNGPLVDESGRVIFADFNPSKRPWSMASDDSNNSWNP
                     AYNCFDRPGKKCISFTLHINGKDHDLLAVDKIHVDLNWRYLNEYLDHISANDEVLFLK
                     QGHEIIAKNKLAREKLIIYNSEGNYNIIDSVDTEYIEKTSAVPNNALFEIYFYYPGGN
                     LLNASDKLFYLPFAFIIIVLLVVYLMTTRVFRRQFSEMTDLVNTLAFLPDSTDQIEAL
                     KIREGDAKEIISIKNSIAEMKDAEIERANKLLSLISYDQESGFIKNMAIIESNNNQYL
                     AVGIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCREN
                     VQTFTRKIATVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRNHMF
                     SEFIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRTILGAEALCRWV
                     SAERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSP
                     WQLNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRVSI
                     DDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGV
                     ESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            complement(34501..35412)
                     /gene="gsiD"
                     /locus_tag="ACHKFK_001837"
     CDS             complement(34501..35412)
                     /gene="gsiD"
                     /locus_tag="ACHKFK_001837"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO9052190.1"
                     /translation="MRLFNWRRQAALNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(35415..36335)
                     /gene="gsiC"
                     /locus_tag="ACHKFK_001838"
     CDS             complement(35415..36335)
                     /gene="gsiC"
                     /locus_tag="ACHKFK_001838"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO9052191.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(36353..37891)
                     /gene="gsiB"
                     /locus_tag="ACHKFK_001839"
     CDS             complement(36353..37891)
                     /gene="gsiB"
                     /locus_tag="ACHKFK_001839"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO9052192.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKNVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(37911..39782)
                     /gene="gsiA"
                     /locus_tag="ACHKFK_001840"
     CDS             complement(37911..39782)
                     /gene="gsiA"
                     /locus_tag="ACHKFK_001840"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO9052193.1"
                     /translation="MPHSDELDAGDVLAVENLNIAFMQEQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGSVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSILEPLRVHGLLPGKEAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQVVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAVVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(39769..40734)
                     /gene="iaaA"
                     /locus_tag="ACHKFK_001841"
     CDS             complement(39769..40734)
                     /gene="iaaA"
                     /locus_tag="ACHKFK_001841"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO9052194.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLV
                     AGESVLDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMECVSPEIFSTPLRYEQLMAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            40938..42173
                     /gene="moeA"
                     /locus_tag="ACHKFK_001842"
     CDS             40938..42173
                     /gene="moeA"
                     /locus_tag="ACHKFK_001842"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO9052195.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNTDGELEVTTTGHQGSHIFSSFSLGNCFIVLERERGN
                     VDVGEWVEVEPFNALFGGL"
     gene            42173..42922
                     /gene="moeB"
                     /locus_tag="ACHKFK_001843"
     CDS             42173..42922
                     /gene="moeB"
                     /locus_tag="ACHKFK_001843"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO9052196.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
BASE COUNT        10735 a        11356 c        10480 g        10443 t
ORIGIN      
        1 cttaatgtga ttacttcagt accgcccacc accgccaggg cgttgattca ggggctgaaa
       61 cacgatctgc tggcggatga caccgcgcta cgtgcgctca tcccacaacg gctgatcgct
      121 ttcgatgacg cggtacgtag cacgttgaaa gaggaagaaa agctggtcaa ctccagcgac
      181 tggggctacg acgctcaggc ctttgcccgc tggcgaccgg agtacggtta tttcgccaaa
      241 caggcggggt ttaccgttaa aacgtccgcc agccttgcgg ctttatggca ggtggtgaat
      301 caaatcggcg gtaaagagcg ttatttcttt ggcaatattt tgtggcaaac ccgggcgttg
      361 atggaccgcg cgatcggtca taagctggcg aaaggccgcc cggagcgtga atatttgcag
      421 accggagatg cggtggatag ctggaaagtg attgtcgttg aaccgcaaaa acaacttacg
      481 ctgttatttg gcatgaaagc gccggggctg ggacgactgt gttttaccct ggaagataaa
      541 ggtgactatc gtactatcga tgttcgcgca ttctggcatc cgcacggtat gccagggttg
      601 ttttactggt tattgatgat ccccgcgcat ctgtttattt ttcgcggaat ggcaaaacga
      661 atcgccagac tggcagaaca aagcacagat taatcgacaa aacctgcgtt attctttcat
      721 gtttcccatt gcatcatgga ggaaatcacg gaagaatgcg cacgttattt tctcaacaac
      781 agtggattga tatgaaggta ctggttaccg gcgccaccag cggcttaggt cgaaacgcgg
      841 tagagttttt atgccagaaa ggcatcagcg tgcgagcgac cggtcgcaac gaggcaatgg
      901 gtaaattgct ggaaaaaatg ggcgcagagt ttgttccggc ggatctgacc gagctggtct
      961 catcacaagc taaagtgatg ctcgcaggca ttgatacgct gtggcactgc tccagcttta
     1021 cctcgccctg ggggacacaa caggctttcg atctggctaa cgttcgcgcc actcgccgcc
     1081 tgggtgaatg ggctgtcgcc tggggtgtgc gtaactttat tcatatctct tctccctccc
     1141 tgtacttcga ttatcaccac catcgcgata ttaaagaaga ttttcgccct caccgcttcg
     1201 ccaacgagtt tgcccgcagc aaagcagcca gtgaagaagt gatcaatatg ctttcgcagg
     1261 cgaatccaca aacacgcttt actattctgc gcccacaaag tctgttcgga ccgcacgata
     1321 aagtctttat cccccgtctg gcgcatatga tgcatcacta cggcagtatt ctgttaccgc
     1381 atggcggcag tgcgctggtc gatatgacct actatgaaaa tgccgtgcac gccatgtggc
     1441 tggcaagcca ggaagcgtgc gataagctac cttccgggcg tgtgtacaac atcaccaacg
     1501 gcgaacatcg cacactgcgc agcatcgtgc agaagctgat cgacgagttg aatattgact
     1561 gtcgtattcg ttccgtcccc tacccaatgc tggatatgat cgcccgcagc atggagcgtt
     1621 taggtcgcaa gtcagctaaa gagccgtcgc taacccacta cggcgtctcc aagttgaatt
     1681 ttgactttac gctggatatt acgcgcgcgc aggaagagct aggttatcag ccagtcctta
     1741 ccctggatga aggtatcgag aaaactgccg cctggctgcg cgaccacgga aaactgccgc
     1801 gctaatcctg cccgtatttc tccagcaacg cttcggcaat cgcctgagtt tctgcatccg
     1861 cttcgccgtt atataacgtc gggcggaaat gcatctggaa cgccataatc acgcgccgct
     1921 gctcgcgcgg agtcatatca ggctttacgt cataaccata acgcgccaaa agctccagca
     1981 atgacgcggt ctctaccggt gtgtgtggcg cgcgcccggc aaggtaaaag ttcacccgct
     2041 gcgcatccgg ccatgcacca atcccctgct gtgccagttg ctgccagggg aataatggcc
     2101 ccggatcgtc tttgcgctgc ggcgcaatat ccgcatgtgc tactacgttt tccggcttga
     2161 tgtgataacg ggcaataata tctttcgcca gcggaataag cgcctgaatc tgtgccggtt
     2221 caaacggggc aaaatatttc actccggctg atttctgcca gccacggttt tccagctcaa
     2281 tgccaataga ggtgtcgtta aggcgcgttg ccccgcgcca ggcgctaatc cccgcatgcc
     2341 aggccagttc ctgttccggc accagttgcc agatgcgcgg tttaccgttg tatcgcggtg
     2401 gcaccgcagg gaccagataa tgcgagctga cctgcttatc ggtcagtgtc gccagcgagc
     2461 tatcaaaatc atctgcggtg tagtggatca ccagcacttt aatgcgcgga tacgccgcct
     2521 gcgcctggcg tcgggtatca agctgatatc cctctttctc gacaatgcct ttttcgcctg
     2581 tgcaccctgc cagtaacaga gcggcagcga ccagccagaa gactcttctc atcgacgcgt
     2641 tttcaccgcc gtaccgctga cgctaaccat cagcatactg ccgttttgcc cgaccgtttc
     2701 gtagtcgata tcaataccga cgacggcatc agcccccagc gctcgcgcct gggagccgag
     2761 ctcctcaaag gcaatctccc gcgctttacg cagttctttt tcgtaggcac cggagcgtcc
     2821 gccaacgata tcgcggatac cggcaaagaa atcacggaaa atattcgcgc ctaaaatcgc
     2881 ttcgccagtc accacaccgc aatattcaac gatggtctgg ccttccagag ttggggttgt
     2941 agaaaattgc atgatcgatc tccttgtttg ctaagccttc gatcttcaaa gcattatcgg
     3001 actgatacgc tattattgaa aggatatcat tattatcgta gaacgctttc aaggtgttcg
     3061 cttataagga aatcatcatg cgctatagca aattgaccat gcttatccct tgcgcactgc
     3121 tactcagtgc ctgtaccacc gtcacgccag cttataaaga taacggcccc cgcaccggtt
     3181 cctgtgtcca gggggggcct gatagcgtgg ctcaacagtt ctatgactac cgcattcaac
     3241 atcgcagcaa tgacattacc gctctgcgcc cctatttgag tgacaaactg gcaaccctac
     3301 ttagcgatgc cagccgcgat aatagtcatc gtgaattgtt aagcagcgat ccgttctcca
     3361 gtcgaaccac cttgccagac agcgcccacg tagccagtgc atcgactatc cctaatcgtg
     3421 atgcccgtaa cattccattg cgcgtggatc tcaagcaggg tgaccagggc tggcaggatg
     3481 aagtgttaat gattcaggaa ggccagtgtt gggtcatcga cgatgtgcgc tacttaggcg
     3541 gtagcgttca tgccacagcg ggtacgctcc gtcagtccat cgagaatcgc taacgtcttg
     3601 atcaaccaat aaacccctta aattgtccgg catttcgtga ggaatgccga catttatgct
     3661 cgccgaccac cgcccccgtt attttgtgct atgtttattg aataatgcgc tttgctttta
     3721 acttttaaag cagaaatatt gcataattat tctgtcaaag gtactatctt cgacctcaat
     3781 agctatcaga ctgccagtat acgagtgtca atgagtattc aattaaacgg cattaattgc
     3841 ttctacggcg cgcatcaggc gctgttcgat atcacgctgg attgcccaca gggcgaaacg
     3901 ctggtgttac ttggccccag cggcgcgggt aaaagctcgc tgctgcgtgt actcaatctg
     3961 cttgagatgc cgcgctccgg tacgctcaac attgcaggca accatttcga tttcaccaaa
     4021 accccctctg acaaagcgat tcgcgatttg cgtcgtaacg ttggcatggt gtttcagcaa
     4081 tacaacctgt ggccgcatct gaccgtgcag caaaacctga ttgaagcgcc ctgtcgtgta
     4141 ctggggttga gtaaagatca ggcgctggcc cgtgcagaaa aactgctgga acgtctgcgt
     4201 ctcaaacctt atagcgatcg ttacccgctg catctttctg gaggtcagca gcagcgtgtt
     4261 gctattgccc gtgcgttgat gatggaaccg caggtactgc tgttcgatga accgaccgcc
     4321 gcactggacc ccgaaattac ggcacaaatc gtcagcatca ttcgtgagct ggcagaaacg
     4381 aatattaccc aggtgatcgt cacccacgaa gttgaagtgg cgcgtaaaac cgccagccga
     4441 gtggtgtata tggaaaacgg tcatatcgta gaacaaggcg acgcgagctg ctttaccgag
     4501 ccgcaaaccg aagcatttaa aaactatctc tctcactaag attcgggata acaacaatga
     4561 aaaaagttct gattgccgcg ttaattgcag gttttagtct ttccgccaca gcagccgaaa
     4621 ccattcgttt tgccaccgaa gcctcctatc ctccgtttga atcgattgat gcaaacaacc
     4681 agatcgttgg ttttgatgtc gacctggcgc aggcattgtg taaagagatt gatgcaacct
     4741 gcactttctc taaccaggcg tttgacagcc tgatcccaag cctgaaattc cgtcgcgtag
     4801 aagcagtgat ggcgggcatg gatatcactc cggagcgtga aaagcaggtg ctgtttacca
     4861 ccccgtacta tgacaactct gccctgtttg tgggtcagca aggcaaatac accagtgttg
     4921 atcagctgaa aggcaaaaaa gtcggcgttc agaacgggac gacacaccag aagttcatta
     4981 tggataagca tccggaaatc actaccgtgc catatgacag ttaccagaac gcaaaattgg
     5041 atctgcaaaa cggtcgtatc gacagcgtat ttggtgacac cgcagtggta accgaatggc
     5101 tgaaagataa cccgaaactg gcggcagtgg gcgataaagt gaccgataaa gattacttcg
     5161 gtactggcct cggcatcgca gtacgccagg gcaacactga actgcagcag aaactcaaca
     5221 ctgcgctgga aaaagtgaag aaagatggca cttacgaaac catctacaac aaatggttcc
     5281 agaagtaatt cctgatgaat gaattttttc ctttagcaag cgccgccggg atgaccgtcg
     5341 gccttgccgt ttgtgcattg attgtcgggc tggcgctggc gatgttcttt gcggtatggg
     5401 agtctgcgaa atggcgtcct gtcgcgtggg caggttcagc gctggtaacc attctgcgtg
     5461 gcctgccaga aattctggtg gtgctgttta tctattttgg ctcctcgcag ctgctgctga
     5521 cgctttcgga tggcttcact atcaatcttg ggttcgtgca gatcccggtg cagatggaca
     5581 ttgagaactt cgacgttagc ccgttccttt gtggtgtcat cgctctgtca ctgctgtatg
     5641 ccgcctatgc ctcgcaaacg ctgcgtggtg cattaaaagc ggtgccggtg ggtcagtggg
     5701 aatccggtca ggcgctgggg ctgtcgaaat cggctatctt tttccgtctg gtgatgccgc
     5761 agatgtggcg tcatgcgctg ccgggtctcg gtaaccagtg gctggtgctg ctgaaagata
     5821 ccgcgctggt cagtttgatt agtgtgaatg atttaatgct acaaaccaaa agcatcgcta
     5881 cccgtacaca ggaaccgttt acctggtaca ttgtggcggc ggcgatttac ctggtgatca
     5941 ccctgctcag tcagtacatt ctcaaacgca ttgacctgcg cgcgacacgt tttgagcgga
     6001 ggcccagcta atgtttgagt atttacccga actgatgaaa gggctgcaca ccagcctgac
     6061 gctaaccgtt gcctcgctga ttgtggcact gattctggca ttgattttta ccatcatcct
     6121 gacgctgaaa acgccggtgc tggtgtggct ggtacggggc tatatcacgc tgtttaccgg
     6181 tacgccgctg ctggtgcaga tcttcctgat ttattacggg ccgggccagt tcccgacgtt
     6241 gcaggagtat ccggcactgt ggcatttgtt gtcagaaccg tggttatgtg cgctgattgc
     6301 gttgtcgctg aacagtgcgg cgtacaccac gcagctgttt tacggtgcga ttcgcgcgat
     6361 cccggaaggt caatggcagt cctgtagcgc cctgggaatg agcaaaaaag atacgctggc
     6421 gatcctgcta ccgtacgcgt ttaaacgctc gctctcttct tattccaacg aagtggtgct
     6481 ggtgttcaaa agtacctctc tggcttacac cattacgctc atggaagtga tgggatacag
     6541 ccagttgttg tacggacgca cctacgatgt aatggtattt ggcgcggcgg ggattattta
     6601 cctggtcgtc aacggcctgc tgacgctgat gatgcgtctg atcgagcgta aagcactggc
     6661 attcgaacgg cggaattaat gtagctgcat gctgatgccg gatgcggcgt ggacgcctta
     6721 tccggcctac atagtgcata aattcttatt atgaagacgg acaacccact aagctgtccg
     6781 tcttttttta tttcatataa attatttact catatttatt gcatataaat tcacttaatg
     6841 gcattgttat cccatgccgc agacacggcc aaaaatcata agattgacag acgggagttc
     6901 catcatgaaa aagttagttc ttgccgcttt acttgcttct tttactttcg gtgcttctgc
     6961 cgcagggaaa atcaattttg gcgtttcagc cacctatcca ccctttgaat ctataggtgc
     7021 taataatgag attgtcggct ttgatatcga cctggcaaaa gccttgtgca aacaaatgca
     7081 ggcagaatgt acttttacta atcacgcgtt cgacagcctg atcccgtcac tgaaattcag
     7141 aaaatatgac gccgtaatct ccggaatgga tatcacgccg gagcgtagca aacaggtatc
     7201 gtttaccacg ccctactacg aaaactcagc cgtcgtgatt gccaaaaaag atacctacaa
     7261 aacgtttgcc gatctgaaag gcaaacgtat tgggatggaa aacggcacca cgcaccagaa
     7321 atatattcag gatcagcacc cggaagtgaa aactgtctcc tatgacagtt atcagaatgc
     7381 ctttatcgat ctgaaaaatg gccgtattga tggcgtattt ggtgacaccg cagtggtaaa
     7441 cgaatggctg aaaaccaatc cgcaactggg cgttgctact gaaaaagtga ccgatccgca
     7501 atacttcggc accggcctgg gtatcgctgt acgtccggat aacaaagctc tgctggaaaa
     7561 actgaataac gcgctggcag caattaaagc tgacggtaca tatcagaaaa tcaatgacca
     7621 gtggttccca cagtaagatt cagaaagccg cacctgcggc ttttttattg cttcaccagt
     7681 agcgtcagca cttcatagtg cgcggtgtgc gggaacatat caaaaagctg tacccgttcg
     7741 atgcgataac caggcagttc gcggatatct ttcgccatag tttgggcgtt acagctggag
     7801 tagataataa aacgcggtgc catcgttgag agataatcac acagcggttt accgatgccg
     7861 cggcgcggcg ggttaaccag caccagctcc ggcacttccc cctgagcggt ggcaaactgc
     7921 gtagagtcca gcgcctgaaa ttgcaaacgc gttaagccca gttcagcggc tgactgcttc
     7981 gcacaggcaa tggcctctgg tgcaatttcg atcccggtta actgcatgtc aggcgtcgcg
     8041 cagtgtaaac caaagccacc cacaccacag aacagatccc acatatgttt aaccggcagc
     8101 tgtcgtatcc agtcgcgcgc ggtggcgtac agctggctgg cgaccgccgg attagtctgg
     8161 aagaaacttt gcggacggat ccacagcggt acgtcattaa aacgctctgc cagtgcctgt
     8221 tgttcggtca ggtagatctc cgtctcccct tccataatcg ccatatgtac cggctgaata
     8281 ttgacggtaa taactttcag ctgcggtaat tgttcctgta accacggcag cgccttacgc
     8341 agttgcgcca gtttggtatc agaacgcagt acaaaacgca gcatcatgcc gccatcgctc
     8401 tggctttcag tcagcagaat gtatttcagt tcaccacgtt tacgcgccac gttgtagggc
     8461 gttaaccccg cacgggcgat aaacggtttt agcgccgcaa aaacgggcgc aaatgaggct
     8521 ggataaagcg ggcagtcaca aaggtcttcc ggtgtaccat cccgatgcag cataccgagc
     8581 agtggttttt caacgctacc actcaccacc attttggctt tattacgaaa cccttgttcc
     8641 gggcctgaca ccggcgcaca ccattcctca accggaaaat cggcgagcag atttttaaga
     8701 tcggcggctt tagcggagag ttgctctgga atcggctgcg ttatccactg acaggaacga
     8761 cagcgacccg cgtcgtaaag tgcgcactgc atacattgac cttcacatca tcaggggcga
     8821 cgattataca cattattgca actggaagaa ccgccgactg gtcgagggaa cgaacagcag
     8881 catcaggatg agcatatccg gcagcttctg cagcatcagg ctatggaaga tttcacgttt
     8941 tgattcaccg ggaatgctaa acagctccgg ataaccatac cccagcgagg ccgcccacaa
     9001 gtaacttgcg gctgtgattt gtgtcaacag atacagccag cgcgcccagc gacgcccttt
     9061 caccagtgaa aacgcacacc agatctcaat gaaaaccagc accagactgc tcaaaaagac
     9121 cagcgttaaa ctccaggttt gtacgctgcg atgaatgaac tcgccaatac cgcgcacgcc
     9181 cagcgtattg aaaatcatca gcacgtcgag gccacggatc ataataatgg cgagcgccgc
     9241 cacctgcacc agcgcaggca cattcaggcg agcatgagat gaagatgttt tcttaaaaaa
     9301 tcccaacgtt tcgtcttcca tgaaaacaat gccgcgacat gcgcggcatt atgtagccag
     9361 gttggcaaat tttagtgtct tcagccacgt cttgcacgct ggatgtcgcg tatccgctgt
     9421 ttttctgcgc gcgccatcag ataccaggcg ataaatccga caattccgac cgcgccgagg
     9481 atcaatgttg ccagggcgtt gatttccgga ttcaccccca tccgcacgct ggaaaatacc
     9541 agcatcggta acgtggtggc tcccggcccg gaaacaaagc tggcgataac cagatcatca
     9601 agcgacaaag taaaagccag taaccagcca gaaatgatcg cgggcatgat catcggtagc
     9661 gtaatgacga aaaacacttt cagcggcgtc gcaccaagat ccatcgctgc ttcttctatc
     9721 gagctatcca gttcccgcag acgcgacgaa ataacgactg ccacataagc cgtacagaaa
     9781 gtgacatgcg ccagccagat ggtgagcata ccgcggtccg caggccagcc aatagcatga
     9841 gcaagcgcga cgaataacaa caacagcgac aaacccgtga tgacatctgg cattaccagc
     9901 ggtgcggtga tcatgaaggc aaaaccattt gatccgcgaa acctgccaaa gcgcaccagc
     9961 acgaccgccg caatagtccc gaggatcgcc gccgccgtcg ccgcacaggc cgcaattgtc
    10021 agacttaaac caaccgcact catcattgcg tcatcgcgca ataattcgcc ataccagcgc
    10081 gttgaccagc cggcccacac cgtcactaac ttcgagctgt taaacgaata gataacaagc
    10141 atcagcattg gcgcgtagag aaaggtgaag cccaacagca aaatcacaat ccgccagggc
    10201 gaacgaacca ccggtaaatt attcatccgt gttctcccac gcttttttgc tgatgtttgt
    10261 ggaaccacat tatcggcata attagcagca gcaacatgat gatcgctacc gctgaggcca
    10321 ccggccagtc gcggttgtta aagaactcct gccacagcac gcgcccgatc atgatgctgt
    10381 ccgggccacc aagcagttcc gggatcacaa actcgcccac agccgggata aacaccagca
    10441 tcgatccggc aataatccca cctttagtca gcggcacgat cacagtaaag aacgttttca
    10501 gcggtcgtgc accgagatcc agtgccgctt ctaccagcga ataatcaata cgaatcaacg
    10561 cggtgtaaat cggcagcacc ataaacggca cgtaggcgta aacaatgcca atataaaccg
    10621 caaagttggt atgcagaatg gtcagcggtt gatcgataat ccccagccac agcagaaaat
    10681 tattcagcac accgttgttt tttaatattc ccatccaggc gtagacacga atcagaaacg
    10741 aggtccacga cggcaggatc accagtagta ataagatatt acgggtcgaa ggcttactgt
    10801 gcgccaccgc ccacgccagc ggatagccga tcagtaaaca gcaaatagtc gaaatcgccg
    10861 ccacctgtaa cgactggaga taagcatcaa aatagagcgg atcgtcggtc agttgcagaa
    10921 aattaccgag attaagagtg atagaaagtt gcccgtcagc ccactccatc aattcggtat
    10981 aaggtggaat agcgcgcgcc atctccgcca ggcttatttt aaagacgatc agaaatggca
    11041 gcagaaacag caagatcaac cagatatatg gcaacgcaat gaccagtttg cgcccatgct
    11101 tcatttgcag ctgcgacaac cacagcttaa atccgcccgg cggtttcgac tgggcagcag
    11161 gttcaagtgt actcatcttt gctccttaaa ccgtcagcac cacacagctg tccacttccc
    11221 agcacaaacg tacttcgtcg ccccaggtcg gtaacccttt acgatggcga tgggcgtttt
    11281 gtagctgggc gctaatcatc tgcccacttt tcaggcgaac gtgatacacc gaaagatcgc
    11341 cgagataggc aatgtgtatc acctccccca ccgcgaagtt acaaccattg gcgggcggtt
    11401 cctcgcaaag catgattttt tccgggcgaa gcgccacatg caccggcacg ttatcgacca
    11461 ctgaagcatc cacatcgact ttcagtggat gcaccagccc cggcgaataa agcaccagac
    11521 catcttcctg acgctctttg agtacacctt caaagacatt aaccgaaccg ataaattcag
    11581 cgctgtagcg agtggtcgga tgctcgtaga tctcttccgg ctcgccaatc tggacaaatt
    11641 tcccacgatt cataatggcg atgcgccccg ccatagtcat cgcctcttcc tgatcgtgag
    11701 taaccattac gcaggtcaca ccgacgcgct ccagaatatc caccacttca agctgcatcc
    11761 ggtcacgcag ctttttatcc agcgcgccca tcggctcatc gagcagtaat agtttcgggc
    11821 gcttggcaag gcttctggcc agggccacac gctgacgctg accaccggaa agctgatgcg
    11881 gtttgcgttt ggcgaactcc tgcatatgca ccagcccaag catctcattg acccggctgg
    11941 caatttccgc tttcggtagt ttgtcctgct tcaggccaaa agcgatgttc tgttccacgg
    12001 tcatatgcgg aaacagcgcg taagactgaa acatcatatt gatggggcgc aggtaaggcg
    12061 gcacctgtga caaatcgacg ccatcgagca taatctgccc ggcagaaggt tgttcaaaac
    12121 ctgccagcat acgcagcagc gtggacttgc cacagccgga tgcgcccagc agcgcgaaga
    12181 tttcaccttt gtagatggtc aggctgacat catccaccgc atgttgacca tcgtaggatt
    12241 tggtcaggtt acggatttct aatagcggcg tcagcgcctt acgggttttc gcctgcgggc
    12301 gagggatagc gtcattcacg gctgctctcc ggcattgaat gtgtgcaagc gcaccatgat
    12361 ggtgcgaatt gccggcgggt gtggtgcgcc cgtccggcat ctacggctgc ggattatttt
    12421 ccgctcttca ctttggtcca cgcgcgggtg cgcacacggt cgattttcgg gtcctgcact
    12481 ttcagagtga acagcttcgc acgaacatcc gcaggcggat aaatacccgg gttatcacgg
    12541 acttccgcac tcaccagcgg cgtggcttct ttattagcat tagcatagaa cacatggtca
    12601 gagatatgtg ctaccacatc aggacgcagc aggtaattca ggaactgata ggcttcgtct
    12661 ttgtttttgg catccgcagg catggcgaat acatcaaaga acgccatcgc cccttctttt
    12721 ggaatcgaga acgagacatt cacgccattc ttcgcttcct tcgcgcggtt tgacgcctgc
    12781 cagacatcgc ccgcccagcc aatagcgaca cagatgtcac cattcgccag gtcattgatg
    12841 tattgggatg aatggaaata acgaatgttc gggcgcagct ttaacagcag atcggtagcc
    12901 ggtccggtgt aatcatccgc tttagtgctg ttaggatctt tgccgagata attcagcacg
    12961 gtagcaaaca cttcttctgg cgcatccagg aaagagacac cgcagctttt cagtttttcc
    13021 agattttcag gtttgaggct caagtcccag ctatcgacgg gcgcgttttc gcccagcacc
    13081 gctttaactt tatcgacgtt atagccaatc ccggtcgtcg cccacatata aggcatagca
    13141 aatttattgt cgggatcgtg tttggcgacc aacttcagca gctccggatc gagattcttc
    13201 cactccggca atttgctttt atccagcggc tggaaaactc ccgctgtcag ctggcgctca
    13261 agaaagcttg ctgacggcac caccagatca aagccggtgc tccccgccat taatttgcct
    13321 tccagtactt cgttagaatc gaaaacatcg tagacgactt taataccggt ttctttttca
    13381 aagttggcca ccgtgtccgg ggcgatataa tcagaccagt tataaatgtg gagtgttttt
    13441 tgttcagccg cgagcgtgct gacagagacg gccatcagag cccccgcaac cagacccgat
    13501 agccattttt tatttaaggc ggtcatatct ctttccttct gaaaattcgt taacaaacga
    13561 acaaaagttg tcacactgaa agatatgcaa taaacgtgca tatttttatc ggcgcggtgt
    13621 aagaaaagag aactctctcc cggcctgcac tgctcactat ataaaagcat cgcaagaata
    13681 actttagcca gagtttgaga ctatagctcg gattaaaaaa cggtatttaa caaattttta
    13741 tgcgtatttg ctctatgtga taaagccaaa tcggcacaag agaagtgaat taatctttaa
    13801 taaaaggtta aaaacagcgg ataattttgc ggcacgcagt cactgcggca gcgactgcgt
    13861 gctcgaaagg agttatcagt ggaggaaata gttttcggta acgttatttt gcccttcatc
    13921 gtcagtcggt aacagcagtt gatgggcatt agcttcaaga atgaccatcg aaatctgctc
    13981 ttcgctctga cgcacgaacc atgcaaactg ctcataggtt acgccctgca taacggataa
    14041 agactggcaa accaccagct ttggcagatt atcatcctgc atatcaagaa atgctttcac
    14101 ggtcagcgaa ctggcattga tggctgataa atccgccgcc agcggcaata cggctgaagg
    14161 tcggacttcc gccatggcag aaaacaggat cgtgttatcg atcatatcga ttttagcatc
    14221 aaagacaccg tcgaaattct gcatatgggg cagatgcaga gcctgacagt tatcacattc
    14281 aaaaaaactc atccccaggt catcgagcca ttgacgcaac gtatccagac caggaacgac
    14341 cagcgatgtc ataattttgt acaacctctt ccgataaaaa gaccggcaca gcttacgcaa
    14401 aaagcgcagg caaaaccatg atcagtaatg tgattgcgac taaccacccg ttttcaggca
    14461 atattctgtc gtagcgtggc gttcgatcca gcggatcatt ttacccgcga tgtcgatacc
    14521 ggtggttttt tctattcctt ccagccctgg cgacgcattc acttccatca ccaatggccc
    14581 gcgattagca cgcagaatat ccacaccagc aacgtccagc gccatcgttc gcgcggcttt
    14641 tatcgcgatt tcacgctcct gtggtgtgat actggcgacg cttgccgcgc cgccgcgatg
    14701 caaattggaa cgaaaatcgc cctctttcgc ccgccgttca atcgcagcga ccacttcatc
    14761 gccaacaacc agacagcgga tatcgcaccc ttgcgcctct ttgatatatt cctgcaccag
    14821 aatatgcgcg ttcagaccgc ggaaagcgtc aatcacgctt tccgccgcct gacgcgtctc
    14881 cgccagcacg acgccaattc cctgcgtgcc ttcaaccagc ttgaccacca gcggcgcacc
    14941 accgaccatg tcgattaaat cgctggtatc atccggcgaa tgcgcaatgc ccgtgacagg
    15001 caggtcgatg ccctgacgcg ccagcagttg catggaacgc aatttgtcac gcgcccgggc
    15061 aatggcgacc gactcattga gcggatagct ccccagcatc tcgaactggc gcagtgccgc
    15121 cgtcccataa aaggtaatgg cggtgccaat acgcgggatc actgcgtcaa aatggggtaa
    15181 cttgcggcct ttgtagtgaa tagaagacgc cgcaggattt atgttcatgt agcaagaaag
    15241 cggatcaaga atttcaacca ggtgaccgcg ctgtatagcg gcttcacgca gccgcttaca
    15301 cgaatagagc gttccatccc gggacaatat ggcaattttc accctgcacc tctctgtcag
    15361 acctggtcaa aagcccgcgt atcatacatc gacgtggcag ttttagcgcg tcgcccaacc
    15421 ctgtttgtgc aaataatcca gaataaatgg gcggctttct ttaatgattg ttcggcggat
    15481 atgatcgctc caggtatccc ggcgattatt gctgccacgg gtgaggtaat attccgccag
    15541 ttgctcgtca tactgcgcca gtgcgtcttt attcagcggt tgatagctgt tttcatgcac
    15601 caaaatggag gccggtaaac gcggcttaag atccggatta tccgcaggcc agccaaggca
    15661 cagcccaaac agcggcagaa catgctgcgg taatttcagc agttccgtca ccgcttcaat
    15721 attattgcac aggccgccga tatatacccc acccaatccc agcgattccg ctgcggttaa
    15781 tgcattctgc gccatcattg ccgtatcaac gacaccgagc aacagttgtt ccgccaggcc
    15841 gagctgagca tccggacaga tctgtaaatg gcggttaaag tcggcacaga acacccaaaa
    15901 ctccgccgct tgtgctacgt gtttttgccc gccggtcagc gtcaccagtt cttcacgtaa
    15961 cgctttgtcg gtaatgcgaa taatgctact gcactgcaaa aaactggaac tggacgtcgc
    16021 acgggcgctg ttaataatcg cctcacgctg cgcttcggaa atgggttcat cagtgaaatg
    16081 gcgaatggag cgatggccac aaataagttc aatggttggc gtcattatct ttttctcttt
    16141 ctgaacgtga atattgcggt ggatggttca tcagctgtgg ggcaagacgt tttgccacct
    16201 gaagaataac caccaccgca gcgggaatca tgagcaaaac accgagaaaa atcatcagaa
    16261 tctgcacttc tggccgagaa aatggctcag gtagcgacag ggagtcgctt accgacagca
    16321 gcgccaccgc cagcaacatc attccgataa attccagtat caacacgctt ttaggcaatt
    16381 taccgatcgc gcgcatacgc ttccctctgc aaagtgagcc ttcagtctaa aacttttcac
    16441 tgcattgtgt ttaacagtta tagcttttag caattaatgc aacaggttaa acctactttc
    16501 agcgaataca ttttagcgtg atcattacag gcataaatct atgaggagag aaataatgca
    16561 aaccgttatt tttggtcgtc cgggttgccc ttactgtgtg cgtgcaaaag atctggccga
    16621 gaaattgagc aatgagcgcg atgatttcca gtatcagtat gtagatattc gtgcggaagg
    16681 gatcactaaa gaagatctac aacaaaaggc aggtaaaccc gtagaaaccg tgccgcagat
    16741 ttttgtcgat cagcaacata tcggcggcta taccgatttt gctgcatggg tgaaagagaa
    16801 tctggacgcc tgatcgtctg acaagccctc gcgttgaggg ctttattgat tttttctgtg
    16861 ctgtggttta aacaaactac tgataaataa gaaacacaat gcccccagcg cacaccagaa
    16921 caccgcactt aataaccatg ccagctcttg ccagaatgag cgcgtcggtg aaaaaaacag
    16981 ccgcataatg agcatcgaac agggtgccgc cagcattgcg ccaaacagag gtttcaggac
    17041 ttctctacga tgtgaaaaga agctggcaac tgcaccagga agaatgaaaa atagcaaacc
    17101 gatttcagga tgcccggcag cccgaaaagc gcctttcatg tgcgtcgcca gaaaaagaca
    17161 caccacaatg aagaggacaa aacagcagat tgcccccgcc caacgttgtt tatgtttcac
    17221 tcgttcctcc tgacactgcg tctatcgaac acatttttcg ccagtgtggc gttcagtaag
    17281 ataaagccgc ttcgcattcc atgctaatat aggccaacgc aattcatata gccgttgata
    17341 cctaatgtga ttacactagt aaaatatatt gttactttac tatcgtttag gtgcgctgaa
    17401 tgaatctgcg ccctgaattc tggtaaaaaa cattatcgta aattaccatt tctttcaaca
    17461 gcttactagt aaacaagaag ttagcctccg tgaatataaa cgtcgccgaa ttgttaaatg
    17521 ggaattacat tctgttatta tttgtggtcc tcgcgcttgg gctatgtctc gggaaattac
    17581 gacttggttc gatccaactg ggtaattcca ttggcgtttt agtcgtatcg ctgttattag
    17641 gccaacaaca tttcagcatt aacaccgacg cgcttaatct tggctttatg ctgtttattt
    17701 tctgcgtcgg ggtcgaagcc ggaccgaact ttttttctat tttttttcgc gatgggaaaa
    17761 attacctaat gttagcactg gtgatggttg gcagtgcgct ggtgatcgcc ttagggttag
    17821 gtaagctgtt tggctgggat attggcctga cggccggtat gttagctggc tctatgacgt
    17881 cgacaccggt tctggtcggt gctggcgata cactgcgtca ttccggcatg gaaagcaggc
    17941 aactctcact ggcactggat aatctgagcc tcgggtatgc cttaacctat ttaatcggtc
    18001 tggtgagttt gattgttggt gcgcgttact tgccgaaatt gcagcatcag gacttacaga
    18061 ccagcgccca gcaaatcgcc cgcgaacgtg gcctggacac tgatgccaac cgtaaggttt
    18121 atttaccggt gatccgcgcc taccgcgtcg gcccggagct ggtggcctgg accgacggca
    18181 aaaatctgcg tgaactgggt atttatcgac aaactggctg ctacattgaa cgtattcgac
    18241 gtaacgggat tctggcgaat ccagacggtg atgctgtgct acaaatgggc gatgaaatag
    18301 cgttggtagg ctatcccgac gcccacgcac gactcgatcc cagcttccgt aacggcaaag
    18361 aagttttcga tcgtgacctt ctcgacatgc gtatcgtcac tgaagaagtg gtcgttaaaa
    18421 accataacgc cgtgggcaaa cgtctcgcac aactgaagtt gaccgatcac ggttgcttcc
    18481 ttaaccgcgt cattcgtagc cagattgaga tgccgataga tgacaacgtc gtgcttaaca
    18541 aaggtgacgt tttacaagtc agcggcgatg cccgtcgcgt aaaaaccatc gccgatcgca
    18601 tcggctttat ctcgattcac agccaggtca ctgacttgct ggcattctgc gccttctttg
    18661 ttattgggct gatgatcggg atgatcacct tccagttcag cacattcagt ttcggcatgg
    18721 ggaacgctgc cgggttgtta ttcgccggaa ttatgctggg ctttatgcgc gctaaccacc
    18781 cgaccttcgg ttacattccg cagggtgcat taagcatggt gaaagagttc ggcttaatgg
    18841 tgtttatggc aggcgttggt ctgagcgccg gtagcggtat taataacggc ctgggcgcga
    18901 ttggcggtca gatgttgatt gccggattga ttgtcagtct tgtgccagtg gttatctgtt
    18961 tcttgttcgg tgcttatgta ttgcgaatga accgcgcact gttgttcggc gcaatgatgg
    19021 gcgcacgcac ctgcgcgccg gcaatggaga tcatcagtga tacagctcgc agtaacatcc
    19081 cggcgctggg ctatgcgggc acctatgcaa tcgccaacgt cctgctgacg ctggcaggga
    19141 caatcatcgt catggtatgg ccaggattag gataaaactg aagttgccct gaaaatgaaa
    19201 ttttttttgc acaaccgcag aacttttccg cagggcatca gtcttaatta gtgccactgc
    19261 ttttctttga tgtccccatt ttgtggagcc catcaacccc gccatttcgg ttcaaggttg
    19321 atgggttttt tgttgcctga aatttatcta ctacccagca atcctctcaa ccatcctcaa
    19381 aatctcctcg cgtgatagcg gcttacggtc ggtgacaaaa tgcagcgtca tcccctcaat
    19441 aaacgcatca agcgcgcggg cggttccggg ttcaaaccat tgttcgagcg tttgttgact
    19501 gcgctgcatc cagttttgca ttaccgtttt taatagcggt tttcggctag ccagcgcgta
    19561 gagctggtac atcagctcca tattatccgg cgttgcgacc tgtgagctgt agatcatatc
    19621 ggtgatagcc tggcatgcgc ctggagcatc actaacatcg ctaaaaaatg cctgatattg
    19681 ccgggacatg atctcagtaa aacggctgaa cgcctccagc aacaactcat caattcctga
    19741 aaaatagtag gtcatcgatc ccaacggcac cccggcaagg gtagcaattt tgcggtgtgt
    19801 aacagcatgt attccgtaaa gtttcaccgc ctccagcgtg gcctggataa ttttttctcg
    19861 ccgttgcgga tcgttagcgc gacgcatgtc atttcctctc tctgcaattg tgtacaaatg
    19921 tacacagtct tgctaatgtt gtcttccctc ttcttatttt gacgtggtct atgaccgtaa
    19981 attcttcacg taatgcattg aaacgccgaa cctgggcgct gtttatgttc ttctttttgc
    20041 caggcctgtt aatggcgtcc tgggcaaccc gtacgcctgc tatccgcgat attctctctg
    20101 tctcgatcgc tgaaatgggc ggtgttctct ttggtctgtc gatcggttcg atgagcggta
    20161 ttctctgctc ggcgtggtta gtgaaacgct ttggcacgcg taatgtcatc ctggtcacga
    20221 tgtcctgcgc attgatcggg atgatgatat taagtctggc actctggcta acatcgcccc
    20281 tgctctttgc cgttggtctc ggcgtctttg gggcaagttt tggttctgcg gaagtggcga
    20341 taaacgttga aggtgctgcc gttgagcgag aaatgaataa gacggttttg ccgatgatgc
    20401 acggttttta tagcctgggc acgctggcag gcgctggtgt cgggatggca ctgacggcct
    20461 ttggcgttcc ggcaacggtg cacattttat tggcggcgct ggtaggcatc gcgcctattt
    20521 atatcgccat tcaggcaatc cctgacggta cgggcaaaaa tgctgccgat ggcacccagc
    20581 atggcgaaaa aggcgttcct ttttatcgcg atatccagtt gctgttgatt ggtgttgtag
    20641 tgctggcgat ggcctttgcc gaaggttctg ccaacgactg gttaccctta ttaatggttg
    20701 atggtcacgg ttttagccct acttccggct cgctgattta tgccggtttt accctgggga
    20761 tgaccgttgg acgctttacc ggcggttggt tcattgaccg ttacagtcgc gttgccgtgg
    20821 ttcgggccag tgcactaatg ggggcgttgg gtattgggct gattattttt gtcgatagcg
    20881 cctgggttgc tggggtgtct gttgtacttt ggggactggg tgcctcgctg ggcttcccgc
    20941 tgaccatttc tgccgccagc gataccggcc ccgatgcacc aacccgcgtc agtgtggtag
    21001 caacgaccgg ttatctggct ttcctcgtcg ggccgccgct gctgggctat ctcggcgaac
    21061 attatggatt acgtagtgca atgctggttg tactggcgct ggttattctc gcggctattg
    21121 tcgcgaaagc cgtcgccaaa cccgatacca aaacgcagac ggcgatggag aatagttgat
    21181 gagcattaaa ttaattgcgg tagacatgga tggtactttc ttaagcgatc aaaaaaccta
    21241 taaccgtgag cggtttatgg ctcagtatca gcaaatgaaa gcacaaggca ttcgctttgt
    21301 ggtcgccagc gggaatcaat attatcagtt gatctctttc ttccctgaaa ttgctaatga
    21361 aatagccttt gtggctgaaa acggcggctg ggtagtgagc gaaggcaaag atgtttttaa
    21421 tggcgagctg tcgaaggatg cgtttgctac tgtcgtggaa catttgctga cgcgcccgga
    21481 agtggaaatt attgcctgcg gaaaaaatag cgcctatacc ctcaaaaaat atgacgatgc
    21541 catgaaaacg gtggcggaaa tgtattatca ccgtctggaa tacgtcgata actttgacaa
    21601 cttagaagat atcttcttta agtttggtct gaatctttcc gatgaactga ttccacaagt
    21661 acaaaaagca ttacatgagg ccatcggcga tattatggtg ccggtccaca ccggcaacgg
    21721 cagcatcgat ctgattatcc ccggcgtaca taaagccaat ggccttcgcc aactgcagaa
    21781 attatgggga atagacgaca gcgaagtggt ggtctttggc gatggcggta acgatattga
    21841 gatgctgcgt caggcaggct ttagttttgc aatggaaaat gccggcagcg cggtcgtcgc
    21901 agcggcaaaa taccgggcag gctccaataa ccgtgaaggc gtactggatg tgatcgataa
    21961 agtgcttaaa cacgaagcgc catttaacca ataaaacggc agcacgcccg gcaacacaat
    22021 gcctgaatga agattgcata attcgcggaa attaacccgc tgcattattt caggcgaaat
    22081 gattatgaaa aattgtctac tgttgggcgc gcttttaatg ggctttactg gtgtggcgat
    22141 ggcgcaaagt gtcaccgtcg atgtgccgag tggatacaaa gtggttgtgg ttcctgattc
    22201 agtcagcgtt ccacaagcgg tcagcgtcgc gactgtgccg caaaccgtct atgtggctcc
    22261 tgcgccagct ccggcttacc gcccgcatcc ttatgttcgc catcttgcca gcgtaggtga
    22321 agggatggtt atcgaacacc agattgacga ccatcaccat taattcgaga atgcctgatc
    22381 gcacaaagca atcaggcatt tttttaccct tcgtgagaat ttcccatctg tttatctttt
    22441 aaaaagataa ccatcagcga cagccacaaa atgccgttga caaggttgaa gagattaaac
    22501 agtccgttgc ccccgttcaa ccaggcatgt ttgctgattt caataccgac ggtaaagatt
    22561 agcatttgca gcatccccat cgccgcagaa accgtacctt tactcatatc gctggcaaac
    22621 agggttaatc gcaccagtcc cgcattcgcc agaccaatac cgaaagcata aatacttaac
    22681 ccggcggtca tccataaata cgcgtgcgat gagataaccg ttgccgcagc agcgaccaac
    22741 agaccaatca taatcggcca gccacccata ataatcagcg aacgtacggt gcggcgcgag
    22801 gtcagacgcg ctaacagcaa gttacccgca attaacgccc cgaaaatagg cacttgcagc
    22861 aagccatatt catagctgct caactgctca ccggtaataa tgataatcgg cgactgggcg
    22921 atccacgcca acaacggcag gctaacgaat cccagcgcca gcgcccccgc cacaaagcgg
    22981 ccgttcttca gcaccagctt atagtcacga ccgagttctt tcagcgacag tttctcgcct
    23041 atacgcgtgg cggtttcagg catagctcgt tgcagaccga aaaaggagat cgctgccaat
    23101 gcggcaaaca agacaaacat cccttcccag ggcagcacat ggatccacgc cgcgcccacc
    23161 agcggaccaa gtagtggagc aatcagcgcc acgttcgcca ttagcgcggt gatcttgata
    23221 caaaccgcct cttcgaagga ttcctgaatt gcggcgtatc ccacagcgcc aatgaaacag
    23281 aggcttatac cctgcaagaa gcgcaacagg gtgaattgtt caatgttttg tgccagtaat
    23341 attgccagac aggtgacgat aaaccacacc actcccgcca gcatcaccgg acggcggcca
    23401 atacgatccg acaacggccc caggagccat tgtaaaaaca tcccgcccgc cagatacgca
    23461 gtcatcgaag taggaaccca atcaatgccc gcctgatatt gttccaccac ggccaacata
    23521 ccgggttgaa tcatatcgtt gccgatatag gttgaaaatt cgtaaagcac cagacagaga
    23581 gggaaaagta acgcctgacg tccaagcctg acaccggaag ctaatttatt ttgcatgcaa
    23641 tttcttcgcc aataataatc gcgcaaagtt taataaaagc gcagctaacg agaaagcgaa
    23701 ttttgtagct gaaaccacgg ttaagcacat tcttacatta ttgcgagtat agctacgctt
    23761 tctttaagtt ttatttaacc tttgcccgtt acaatcacct accgtaaaca ggccgcttga
    23821 gggaaataag acgatgccgc tttacccagt ttaacctgca ctttattctc aacgacttgc
    23881 ctgtattggc tcccttttaa tcactttgcg tcgggaagtt atgctggaaa atttgaatct
    23941 ctctctattc tctcttatta acgcaacgcc agactctgcg ccgtggatga tctcgttggc
    24001 gatttttatt gctaaagatt tgattaccgt ggtgccgttg ctggccgcag tactttggtt
    24061 gtgggggctt acagcacaac ggcaactggt gataaaaatc gctatcgcgc tggcggtcag
    24121 cctgtttgtg tcctggacga tgggacatct ttttccgcac gaccgaccct ttgtcgaaaa
    24181 tatcggctat aacttcctgc atcatgcggc ggatgactca ttcccgagcg atcacggtac
    24241 ggtgattttc acctttgcac tggcattttt atgctggcat cgcctgtggt ccggctcact
    24301 tttaatggtg ctggccgtcg tcattgcctg gtcgcgcgtt tatcttggcg tccactggcc
    24361 gctggatatg ctcggtggat tgctggcagg tatgattggc tgccttagcg cccagattat
    24421 ctggcaagcg atggggcata aactctatca acgtctgcaa tcgtggtatc gcttctgttt
    24481 tgcattaccg atccgcaaag gctgggtgcg tgactgatct ctgctaaaaa gtgtagtatt
    24541 gagcggctcg cttcaataac tattcagagg gattatggaa acacgtcgcg aagagcgtat
    24601 cgggcagctg ctgcaagaat taaaacgcag tgataagtta catcttaaag acgccgccgc
    24661 cctgcttggg gtttcggaga tgacgattcg tcgcgatctg aacaaccaca gtgcgcccgt
    24721 cgttttgctc ggcggctata ttgttctgga accacgcagc gccagccatt acctgttaag
    24781 cgatcaaaaa tcccgcctgg tggaagaaaa acgccgggcg gcaaaactgg ctgcgacgct
    24841 ggtagaaccc gatcagaccc tcttttttga ctgtggcacc accacgccgt ggattattga
    24901 agcaattgat aatgaaatcc cttttaccgc cgtttgttat tcactaaata cctttctggc
    24961 gctgaaagag aaaccccatt gccgcgcgtt tctttgcggt ggtgaatttc acgccagcaa
    25021 cgccattttc aaacccatcg attttcagca aacgctgaat aatttttgcc cggatatcgc
    25081 tttttattcc gctgcgggcg tgcatgtcag taaaggcgct acctgcttta atcttgaaga
    25141 gttgccggta aaacactggg ccatgtcgat ggcgcaaaag catgtgctgg ttgtcgacca
    25201 cagtaaattt ggcaaggtgc gtccggcgcg catgggtgac ctgaaacgct ttgatattgt
    25261 tgtgagcgat tgttgcccgg aagatgagta tgtgaagtac gcgcagacgc agcgtattaa
    25321 gttgatgtat taatgacgta taaccggatg acgtttcgcg ccatccggtt atcagaagat
    25381 taagagaacc agctgccgaa ccactgatgg aatttcatca tcacgaaatc ccacatccga
    25441 ccaaagaatc cgccctcttc cacattttcc atcacgatca gcggacgctg ctcaatggat
    25501 ttaccgttaa gctggaaatc aatggtgccg acaacctgac cttttttcag cggtgcggta
    25561 agctgcggtt ccgttaacgt ataactcgcc ttcaggtttt tcagctgccc acgcggtatg
    25621 gtcactgaac ccgcttcgcc tgccccgaga ttcacttcgc tcttatcacc aaaccagacg
    25681 cgctgagtca caaatgtggc atcaggttta attggcgtca cggtttcaaa gaaacggaag
    25741 ccccaggtca ataatttctc agactcatta aaacggatac ggtcagtttt cgcccccagc
    25801 actacggaga ttaaacgcat atcgccctgg gtagccgaag caaccagatt atatcccgcg
    25861 cctgccgtgg ttcctgtctt catgccatca acattcagat tgctgctcca cagcagacga
    25921 ttacggttag gctgacgaat tttgttgaag gtgaactctt tctctttatg aatggcgtac
    25981 tcttccggca catcgtggat caatgcttta cccagcaatg ccatatcgcg cgcggtactg
    26041 aactgccccg gcgcatccag gccgtgcacc gtctggaagg tagtgttggt cagacccagt
    26101 tttttggcat aaccattcat caaaccaata aatgactcct ggctcccggc aacgtaatca
    26161 gccagcgcaa tacaggcgtc attaccggac tggataatca cacctttgtt caagtctgcc
    26221 accgaaacct gatcgcccgg tttgaggaac attaccgatg aaccacgcag tgccggattt
    26281 cccgtcgccc aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc
    26341 ttaagcgcct gcccaaccac atagctggtc ataattttag tcaggctcgc gggatccagt
    26401 ttctcatccg cattgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat
    26461 gcacgcgcat ccacgctcgg cgcttcaacg gtttgttccg ccgcgaatgc cgttggggca
    26521 aaaaggaata aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc
    26581 ataaatgcca cccgagtatc cattcaaaaa aaatacgtta tatcgccgta tgataagttc
    26641 ggtgattaat aacgcacaca caaacttaaa gaaaccgtaa gttggtaaag tttttaaagt
    26701 ttatgcaata tcctgccgct tcgctgcttc tggtgtcatt tttttgccag ctgttgctta
    26761 aaagttaact ttacttcacc atcggagtcc tgttcagcct gtggctgcct gtcggaaaag
    26821 gggtgagtat gattacgctg tggggtcgga ataattcaac caacgtaaaa aaagtattgc
    26881 tgacgctcga agaactggaa ctaccttatg agcaaattct cgcgggccgt gagtttggga
    26941 taaatcacga tgctgatttt ctggcgatga accctaacgg gctggtgccg ttgttgcgtg
    27001 acgacgaaag tgatctcatt ctttgggaat caaacgccat tgtccgctat ctggcggcac
    27061 agtacgggca aaaacgcctg tggatcgact caccggcacg tcgtgcggaa gccgaaaaat
    27121 ggatggactg ggcaaaccag acgctcagta atgctcatcg cgggatcctg atgggattag
    27181 tcagaacacc accggaagag cgcgatcagg ccgccattga tgccagttgc aaagagtgcg
    27241 acgccctgtt tgccctgctc gatgcggaac tggcaaaagt aaaatggttc tcaggcgacg
    27301 agtttggtgt gggcgatatc gctatcgcac cgttcatcta caatttgttt aacgttggcc
    27361 tgacctggac accgcgtcca aatctgcaac gctggtatca gcaactcact gaacgccccg
    27421 cagtgcgcaa agtggtgatg attcccgtta gctaattgcg tgggctaact ttaagtaatt
    27481 ccccactgga ctcgtcggtg agaacgtata aataaccgtc gggtccagtg cgaacatcac
    27541 gaattcgctg ccctctgtcc gttaaaatac ggccatcttc tgtcactttg tcgccgttga
    27601 cgctcatcac aatgacatct ttatctttca gcgcgccaat aaataatttt tgctgccact
    27661 gggggaattt gtcgctgtta tagaaggcca tgccgctcac ggcgggagaa tctttccagt
    27721 aaaaaacagg ttgctcggtc ccggcgacga tctccccttt agcttccggt atcttaaagc
    27781 ccgaatagtt gattccccag gttgccagcg gccagccgta gtttttgcct ttttgcggaa
    27841 tattaatttc atcaccaccg cgcgggccat gttcattcag ccatagtgca ttactccacg
    27901 gattcatcgc cattccttgc ggattacgaa tgccataaga ccagatctcg gcgcgcacac
    27961 cggattcctt tataaaagga ttatcatccg ggatttctcc caggtcggtc agacgcacca
    28021 gtttgccctg taatttatcc agatcctgcg ccgtcgggcg ctgattgttt tcgcccagag
    28081 caataaaaag ataaccttta ccgtcgaata ccatccgccc gccaaaatgg ttaccggtag
    28141 acagttttgg catctgacga aagacggtgc ggaagtcggt cacttttgag agatcatcac
    28201 ttaagcggcc ataacccaca gcagttccgg ctttgccatc atcgccaact tcggaataac
    28261 ttaaccagat gcggcgcgac tgagcaaaat cgggcgctaa aaccacgtcc agcaggccgc
    28321 cctgcccgtg cgcccaaacg tccggcactc cggaaagcgg cgcagataat ccttttcctg
    28381 cttgccagtg acgcaactcg ccgccgcgca gagtgattaa cataccgtga ttatcgggta
    28441 aaaaggccag cgcccaggga tggtcgagtt tgtcttgcag tacttcgaca tttaccgttg
    28501 caggagctgc ccagagagcg gaagaaagac aaataagggg cacaaggaaa aaggattgtc
    28561 gatgcataac gcctccggat tgactctggc ttaaagcgta gtcagtggag gagataacaa
    28621 attcattttt acaaaaactt aaacatgaag ggggagacgc tttctccccc ttagttttca
    28681 ggccttctca agcatggcgt gcttctgcag gctctggata ctcagcgtta agctcatcag
    28741 acaattttca agcttatcgg cgttgacggt aataacagtc gggcaatcat ggtgcccact
    28801 catcaaacat actgcggctg tcgctaatgc ttcttcagca tgatgaagag cactccactc
    28861 ttcctgatcc agatgaagat tcaaccgcag cgatttgtcg tgcagttcgc gattcagttt
    28921 aaaaaagtta tctcgtagat gattgctttc gctgacggac atgtatcctt ttgccttcct
    28981 cagttgcacg tatgcggcga ggtcaacgcg tgcgtcgatc aacctgttca gcagatcgat
    29041 tcgctgtctg tcaacgaaca tacttcgatc ctcctctttc cagccttgtt gcaactaaat
    29101 agtatggctg ttttttgagc aaaatgctgt ccaggatcaa ttcttcacgc cgaattaaca
    29161 aaatttacat ttccttcaac tccgccagcg aatgagattg cgatacaaat tttatccaga
    29221 actctgtaga atccctgccc tgacctttcc tgtaactgaa tattttttaa gctatgagca
    29281 aagtaactcc ccagccgaaa atcggctttg tttcccttgg ctgtccgaaa aaccttgtcg
    29341 attcagagcg tattctcacc gaactacgca ctgaaggtta tgacgtggta ccgagctatg
    29401 atgatgcgga catggtgatc gtcaacacct gcggctttat tgacagcgcg gtacaagaat
    29461 cactggaagc cattggtgaa gcgttgaatg aaaacggcaa ggtaattgtg accggttgtc
    29521 tgggggcaaa agaagatcag atccgcgaag tccacccgaa agtgctggaa atcaccgggc
    29581 ctcatagcta tgagcaggtt ctggagcacg ttcatcacta cgtgccaaaa ccgaaacaca
    29641 acccattcct gagcctggtg ccagaacaag gtgtgaaact gacgccgcgt cattatgcct
    29701 atctgaaaat ttctgaaggc tgtaatcacc gctgcacctt ctgcattatt ccgtctatgc
    29761 gcggcgatct ggtgagccgt ccgattggcg aagtgttaag tgaagcgaaa cgtctggttg
    29821 atgcgggcgt taaagaaatt ctggtgatct cgcaggatac ttccgcgtat ggcgtcgatg
    29881 ttaaacatcg tactggcttc cacaacggcg agccggtaaa aaccagcatg gtcagcctgt
    29941 gcgaacagtt atcgaaactg gggatctgga cacgtctgca ctacgtttac ccttatccgc
    30001 atgtggacga cgtcatccca ctgatggcag agggcaaaat cctgccgtat ctggacattc
    30061 cgttgcagca cgccagcccg cgcattctca aactgatgaa acgtccgggt tctgtagatc
    30121 gccaactggc gcgcatcaaa cagtggcgca aaatctgccc ggaactgacc ctacgctcaa
    30181 cctttattgt tggcttccct ggcgagacgg aagaagattt ccagatgcta ctcgacttcc
    30241 tgaaagaagc acgtctggat cgcgttggct gctttaaata cagcccggtt gaaggtgcag
    30301 acgccaatgc cctgcctgac caggttccgg aagaagtgaa agaagaacgc tggaaccgtt
    30361 tcatgcagtt gcagcagcaa atttccgccg agcgcctgca agagaaagtg ggccgtgaaa
    30421 ttctggtgat tatcgacgaa gtggacgaag aaggcgcgat tggtcgcagc atggcagatg
    30481 caccggaaat cgacggcgcg gtttatctca atggtgaaac caacgttaag ccgggtgata
    30541 tcctgcgagt gaaagtcgag cacgccgatg agtacgattt gtggggtagc cgggtttaat
    30601 taattcaggc cgggcaaaat catttgtccg gcctgcgaat aatgattact gccagctaat
    30661 ctgcgccctg ccgtttttct tactcttata cagccgctca tccgccagca ttttcaagcg
    30721 ttcgacggta gtacattcat ttgaatgcgc tacaccgata ctggtgctta aattaatgct
    30781 tcccacctgg aaatgaacga tttcttttag aatcgatagc aactcttcca gcgtgccgcc
    30841 agtataaatc accgcaaatt cgtcacctcc gaagcggtag actttacctt tatctaccgg
    30901 gatctgtgat ttaaggattt tcaccacttt catcaacact tcatcgccca gcaaatgacc
    30961 gaaggtgtcg ttaatgtttt tgaatttatc gacgtcaaac aggcacaaat aaccgccttt
    31021 ttccggcagc gcttttaaat cttcgtcaaa agattttctg cccatcgctt tggtcagatc
    31081 gtccaggaat gccgagttat acatactgta gagttttttg acatcggcag ccgaaaagaa
    31141 aagcccgcca tccagtacgc gttcaaaatg gttattaata atggcttctt tctccacaat
    31201 accacgatta atcactctgc gtaccagata gaggcaactg gcacccgata tcactgccac
    31261 caatgcggca aagaaaataa tcaatgtaca tacctgaaaa taaacgtctt ttttataagc
    31321 aaatacgtac aacccggtcg gcaaatagga gaagtgctcc attgcttccg aaacggtgaa
    31381 gatggcgttt ttagcgttat attcatcacc ggtcaccgat ttaccgttct tgaacaggga
    31441 gaacatcagg ttctcgctat ccatcgccag cgattttgac agtaaagaaa aacgttccag
    31501 accaagcaaa aatatcagac tacgattgtg agttaccgga tagaggtaat agacgaaggt
    31561 tttaccatca aaagagataa accctgtatt tttgatatgt cgatacttaa caacctgcat
    31621 aatatctgca caataatttt tctcgaactc ctttacctga atacattcat cactggaaat
    31681 cagataagga ccgacatcgt acaaataaga gggtgaaacc gagtcggcgt aagctgtgcg
    31741 taagccaacc aacagctgtt cctccgtagg atttagcgtg ttgatgagat caattgccac
    31801 actacgccga tattcagcaa tatttgccac cgcaaaagca gtatcaataa cggactgttt
    31861 caggccatac acccgttcct ttaccatttg agtgtagatc ccgcaggcaa ctgctgaaat
    31921 catgataaaa atcagcagac tgactgtaag tacgaattta ttgattctgg acatacgtaa
    31981 actactcttt tactaatttt ccacttttat cccaggcgga gaatggcatg ggtttctggt
    32041 acagataccc ctgagcctga acacatccta atttgattaa ttcacgcgca acgtcttcgc
    32101 tctctacgcc ttccgcaatc accgggatcc ggttatagcg tgccagatcg aaaataatgc
    32161 gcacaatttt tcctgcagta ccgaaaatat cgccggtaaa ttgcgaatcc acctttatac
    32221 tgtctggatt aatttcataa aaacgtttca ggtttgacaa accggtgcca aagtcatcaa
    32281 tcgatacccg taccccttgt ttacgcagtt gttcaatatt gagatagaaa tgttcattaa
    32341 ttcgctcgat cacggtttcg gtgatctcaa cacaaaggct gttcgccttc aggccatttt
    32401 ctttcatgat ggtagtaaaa cgctcatgaa agtgtggttc gtttaactgc cagggcgaaa
    32461 cattaatatg cagtaagaaa tcatccttca gcgacgcacg ctgactaaaa tgtctgaatt
    32521 cacccatcgc ggttttaata atttgatagc ccagctcatt gataaacccg atatcttcag
    32581 caatggtaat gaacttcagc ggtgaaataa tcccccgctc cgcagacacc caacggcaca
    32641 acgcctccgc gcccaaaatg gtgcgggtgt taatatcaac aatcggttga tagaccagga
    32701 atagttcgcc aatttccatt gcatggcgaa tattacgcgc aatccagata ttctcttcta
    32761 tttcgttgag tttcgcatca tcacagaaaa taaactctga gaacatatgg ttgcggatac
    32821 tggaaatcgc cagtttcagc ttttctgcgt aactccaggc gttttccccc tgcaaaggtt
    32881 cacaaatagc agacttatgg atgcgcagat tgcggtagcc aaaactgctg tcaaaatcgt
    32941 ttaccgtcgc tatcttacgg gtaaacgtct gtacattttc ccggcacaag agtaaataga
    33001 gatcggcatt aaatgtcacg atatcgcagc attgcgcata tttctcggca attcgctgac
    33061 acaatttcct gacgatttta ttgcgttcat caacaccaaa caccgcttcc acagcttcca
    33121 gaccacacag tttgatgatc cccacagcca gatactgatt attgttggac tcaataatcg
    33181 ccatattttt aataaaaccg ctttcctgat cgtaagagat cagcgagagc aatttatttg
    33241 cccgttcaat ttcagcatct ttcatttccg cgatcgaatt tttgatgctg ataatctctt
    33301 tcgcatcgcc ttcacgaatc ttcagggcct cgatttgatc cgtagagtca ggcaaaaacg
    33361 ccagcgtatt gaccaaatct gtcatttcag aaaattgccg acggaacact cgagtggtca
    33421 ttaaataaac caccagcaat acgataataa tgaaagcaaa cggcagataa aaaagtttat
    33481 ctgatgcgtt caataaatta ccaccaggat aataaaaata gatttcgaat aatgcgttgt
    33541 ttggcaccgc tgatgttttt tcaatatatt ccgtatcgac agaatcaata atattataat
    33601 taccttcgct attataaata atcaattttt cacgagcgag tttattctta gcaatgatct
    33661 catgaccttg ctttaaaaat agaacttcat cattagcgct gatgtgatca agatactcgt
    33721 tcagatatcg ccagtttaaa tcgacatgaa ttttatctac tgctaacaaa tcatgatctt
    33781 tgccgttgat gtgtagcgta aaagagatac attttttacc cggacgatca aagcaattgt
    33841 atgccgggtt ccagctgttg ttagagtcat ctgaagccat cgaccacggt cgtttcgatg
    33901 ggttaaaatc ggcaaaaata acccgccctg actcgtcgac aagcggtcca tttacttgtt
    33961 cctgatgata aacaacgatt ttatcattcg ccctccgcga aaagagataa tatttattat
    34021 catcaagtac cagtcccacc gagtctaaaa atggcatcat ttccattttc agaattacgg
    34081 cgcgttgaat ctcctctgta ttttttaata aatatagtga gtcctttaat gtcagggccg
    34141 aacgttcaat atctttaaag aaatagccaa cgtttttctg aataagttca aagcggctat
    34201 cggcacgttt taccgcgcgt tccgatacca gttgatagtt aatgatgaaa aaaagaccaa
    34261 taaatgacag tgctgccaat aaaaataaaa ttatcagcct tatcttttcg tataaactca
    34321 gcatgttaat acatttaaca aaataactgt ctgaacgctg tgccgctgca aaatagtccc
    34381 ttgctgcggc tttttaacca aattacccga ttctacaaaa tcagcagaaa gttacaacac
    34441 ttgcttaggg caataatttc ctgtgaggtg attacccttt caagcaatat tcaaacgtaa
    34501 ctatccttta attttcggat ccagcgcatc gcgtaaacca tcgcccaaca aattgaacgc
    34561 cagtacggtc agaaaaatag ccagggccgg aaaaacagcg acatgcggcg cgataaccat
    34621 atccgctcgt gcctcattga gcattgcccc ccactctggt gtcggcggct gcgcaccgag
    34681 gccgagaaat gacaggctgg cggcagagat aatcgaggtg ccaatgcgca tggtgaaaaa
    34741 caccacgata gaagagacgg tcccaggcaa gatatgacgc aacaaaatgg tcatatcgct
    34801 ggcaccaata ctgcgtgctg actcgataaa ggtttgctgt ttcaacacca gcgtgttgcc
    34861 gcgcaccagc cgggcaaacg cggggatgga aaaaatggcg actgcgataa tcacgttagc
    34921 aatgccgctt cccaacaccg caacaacagc gatcgccagt aaaatacccg ggaaggcaaa
    34981 caacacatcg caaatgcgca tgatcagccg atcccaccag ccttcataat atccagccag
    35041 caagcccagc aacgtcccga tcgccgcacc gataaacacg gcaaacacgc ccgccgccag
    35101 cgagatttgc gcaccaacca ggacacggct gaaaatgtcg cgccccagtg aatcgacacc
    35161 aaaccagtgc tgcaaagaag gtccgttatt cagattgtca taatcaaaat aattttcggc
    35221 gtcataggga gcgatccagc gtgcaaaaat ggccaccaca atcaataaaa taacgaacaa
    35281 tgcggcggtc atcgccatat gctgacggcg aaatcgtcgc cagaattcat gccacggtgt
    35341 acgtacctgg tcaggtttga ccagtggcat ggcgtttaac gccgcctgac gtcgccagtt
    35401 aaatagtcgc atccttactt gtacctgata gccgggttaa tggcggcgta aagcacatcc
    35461 accactaagt tgataagaat aaattccagc gagaaaagca gaatttccgc ctgaatcacc
    35521 gggtaatcgc gcatttcaac ggagtcaacg agtaagcgtc caagtccggg ccagttgaaa
    35581 actttctcca ccacgatgga accaccgagc aagaagccaa actgtaagcc catcatggtc
    35641 actaccggga tcatcgcgtt gcgtagcccg tgtttgagga caacccaggt ttcgctcacc
    35701 cctttcgccc tcgcggtacg catataatct tcgcttaaaa cgtcgacaaa cgacgcgcgg
    35761 gtaaagcgcg ccatcacggc ggccactgcc gcgccgagcg tcagggaggg taaaatgtag
    35821 tgctgccaac tgtctgctcc cacggtaggc agccagccca gttcaacgga gaatacctga
    35881 attaaaagca tccccagagc aaatgccgga aacgagatcc ccgacaccgc aatagtcata
    35941 ctcaatcgat ccggccaacg gttacgccag acggcggcga taattcccgc cgccatacca
    36001 aatatcaccg cccagaccat actggttatg gtcagccaca gcgttggcat aaagcggctg
    36061 gcaatctcat cggcaaccgg acgacgcgac accatcgaca ggccaaaatc cccctgcaca
    36121 gcattgctga tatagtgcca gaactggtga tacagcggct gatccaaccc cagctgctga
    36181 cgcaccagtt ctataacctg cgcatcagct tcgggcccgg caattaatcg cgccggatcg
    36241 ccgggcagca tgtggacaaa taaaaacacc agcaccgaga cgataaacag cgtcggaatc
    36301 aaccccagta agcgtttgat aacgtaatta agcattccac tccctgcgtt gcttattgca
    36361 aatccgcgtc ttcaaagctg aagccggtgt ctggcatgat ccaaaaaccg gtcaggtttt
    36421 tactgtgtgc cgacaccagt ttttctacca ccagcgggat ccacggcgac tcttgccaga
    36481 tgatatcctg cgccgcctta tacaagcggg tcttttccgc cggatcatta gttttcagtg
    36541 cctgagccag gaagtcatcc acctgtttat tgctgtaaaa cgcggtatta aacagcgttg
    36601 gtggccagtt ctgcgaggca aacagcggcg atagagccca gtcggcttcg ccggttgaag
    36661 ccgaccagcc agtgtagaac atccgcacgc cgctctcttt ttgcccttta ccttcaactt
    36721 ctgccgcccg ctgtccggca tccatcgcag tcacctgggc tttaatccca acctgcgcta
    36781 actgctgctg ggtaaattgc agcactttct gcgcggtgct gtggttatgt gacgaccaca
    36841 gcgtggtact gaaaccgttg ggatatcccg cctctttcag taattcgcgc gctttcactg
    36901 gatcgtaagg ccacggttta taactttgcg cgtaggcgat actcggcggt accacaccag
    36961 tagctggcgt tgcatagccc gcgaaggcca ctttcaccag cgccgggcgg ttaatggcgt
    37021 aattcagcgc ctcacggacc ttcgggttat cgaacggctt ttgcgtcacg ttcatactga
    37081 tataacgctg cataattgac ggactggcca tcaactcgat atttttgttt ttctccagca
    37141 gtgcggcctg ctcgtaggga atggggaaag caaactgcgc ttcaccggtt tgcagcattg
    37201 ccgcgcgggt gttgttatcc gccaccggac gccaggttat gctgtccagt ttgggcaatc
    37261 ctggctgcca gtaacccgcg aattttttca ccttcacaaa atcggtctga ttccaggtat
    37321 ccagttcata cggcccggtt cccaccggat gaaaaccaat ctccttgcca tatttttcca
    37381 gcgctgccgg tgaaatcatc gcggtcgccg gatgggcaag aatattaata aacgctgaga
    37441 atggctgttt gagggtaatc tttaccgttg tcggatcgat cgcttccgtt ttggcaatat
    37501 tcttatacag gttatagcgt ttaagatgat tcgccggatc gctggcccgg tccagattcg
    37561 ctttcaccgc cgctgcgttg aaatcggtgc catcctggaa tttaatgcct tcccgcaatt
    37621 tcacggtgta agtaaggccg tcatcggaaa cggtataact ctccgccagc acgtttttca
    37681 gtttcatctc tttatccaga ccgaacagcc cctggtaaaa cgatttcgct acggcctgag
    37741 ataacgtgtc atttgcgtca tacggatcga gcgtggtgaa gttcgagccc accgccacca
    37801 ccacattttt ggcagcgaat gcacaagatg ccatcaacgc tgtcgcaatg cccagcgcca
    37861 ctaacccact acggtgtaca gctcttgcca ttttattctc cgcctgaatg ttatctacgc
    37921 atgaatgcgt attctgattg tggttgtgcg acgtaatgcc ccggcccgac gcattgcaac
    37981 gagacgactg caacttcttc gccacgcaga tgaatattgc tgggaagatc gtccgacagc
    38041 agtacacgct gcggtcgttg tcgggacggt tcagcgaccg gaactgccgc cagtaattta
    38101 cgcgtataag gatgctgcgg attttcaaag accgcgcgcc gtggaccaat ttcaacaact
    38161 tgcccgagat acatcaccgc cacacgatga ctaatccgct caaccacggc catatcgtgg
    38221 gagataaaca gatacgcaat gccgaaatca cgctggagat cgagcaacaa gttgataatc
    38281 tgcccgcgaa tagaaacatc cagcgccgag acggcttcgt cggcaatgat cacttttgga
    38341 ttcaatgcca acgcgcgagc aatgcagatg cgctggcgct gaccgccgga aaactcatga
    38401 gggtaacgcc aggcatgttc cggtaacaga cccacgcgct ccagcaacca cgcaacgcgt
    38461 gcagccgctt ctttacctgg caataaaccg tgtacgcgca gcggttcgag aatcgaatca
    38521 ccgatggtct gacgtgggtc cagcgaagcg taagggtcct gaaaaataaa ctgaatatcg
    38581 cggcgcaatg cctgaagttt accgggtgac aaggtatcga ttcgctgacc gttaaagata
    38641 atttcgccgc cttgcgattc gaccaggcgc agcaacgccc gcccggtggt ggatttaccg
    38701 ctgccagact cgcccaccag cgatagcgtt tcaccaggcc agagatcaaa actgactttc
    38761 tcaacggcat gcacttcccg cgttacgcga ttcaacaaac cgctgcgcaa agggaaacgg
    38821 gtgaccagat tacgcacccg taaaacaggt tcgccatcca ccaccgtttt ctgctcgatg
    38881 ggtggctcct gtttcgctgg atgttcaagc gatatcaatg ggaaacgtcg gggataatct
    38941 aaccctttca tcgcaccaag ttgcggaaca gcagctaaca gcgcacgggt ataaggatgt
    39001 tgcggtgcat gaaaaatctg ttcgacgcta cccgtttcca ccgcctcgcc ctgatacatc
    39061 accagtaccc gatcggcaat ctctgccacc acacccatat cgtgagtgat aaagataacg
    39121 cccatcgaca tctctttttg caatactttg attaattgca ggatctgcgc ctgaatagtg
    39181 acatccagcg cggtggttgg ctcatcggca atcagcaccg ccgggcggca tgacagcgcc
    39241 atcgcaatca tcactcgctg gcgcatcccg ccagagagtt gatgcggata acgtgaaaga
    39301 atggtttgtg cctccggaat gcgtacctga tccagcatcc gcttcgcctc gaccatcgct
    39361 tcttcacgac tggcgttctg atgcagacga atagattcag caatctgttc acccacagta
    39421 aataccgggt tcagcgatgt catcggctcc tgaaatatca tcgccatatc cgcaccgcgc
    39481 acatggcgca tttgtgcagc gctctgctcg ctaagttcaa ttacttcgcg actgcgccgc
    39541 tgcaacagca ttttatcgca ctgcactaaa ccgcccgcct gttccaacag gcgcatcaat
    39601 gccagcgcag tcactgactt accggagccg gattcgccaa caattgccag cgtctcacca
    39661 cgttgcaggc taaaagagag attgcggacc gcagctattt tctgctgctc ctgcataaag
    39721 gcaatattca gattttcaac cgccagcaca tcaccggcat caagttcatc actgtgtggc
    39781 aacggtgtcc cctttttcac ggtagatacc ggtggttggc gtatcgcctg cgtagcccca
    39841 ggcgcgatac attccttcgg tgttaaacgg tagcgcgaca ttcccttcat ggtcgatagc
    39901 gattaagcca ccgctaccgc caagcgcagg gagtttttcc attactaccc gctcgcaggc
    39961 ttccgcgaga cttaatccgc cgtaatccat taacgcggcg atatcatatg ccgccagcgc
    40021 gcggatgaag acttcgcccg taccggtaca ggaaaccgcc acactggcgt tattggcata
    40081 gcatccggcc cccactaagg gactatcgcc aactcgtccg ggtaatttat tggtcattcc
    40141 gcccgtggac gtggctgccg ccagattgcc gtctaaatcc aacgccacgg cccctacggt
    40201 gcccattttt tgtttttcat ccagtggcgc accgctatgg tcgaggactg ttgccccttc
    40261 ctcgcgcgct gccattagtt gttcataacg caaaggtgtg gagaaaatct ccggcgagac
    40321 gcactccatg ccatgagcaa acgcaaaatt ttctgcccct tcgccaatca tcatcacatg
    40381 cgggctttgc tccatcacca gtcgggcggc aagaaccgga ttacgcagat gactaacgcc
    40441 cgccaccgca ccggctttca gggtgttacc atccatcaca caggcgtcca gttcatgggt
    40501 ttcatcacgc gtaaagacag cgccaattcc ggcgttaaac agtggacact cttccagcag
    40561 acgcaccgct tccgtcacca catccagcac actttcgccc gctaccagca ttttctgccc
    40621 ggtttcaaca atggcagaca acgcctcgat gtagcgtaat tcctgttgca gactcatctg
    40681 cgcgcggcta attgcacctg cgccaccatg aattgcaatg actgctttgc ccatagtatt
    40741 cgtccattaa gggtggctcg ccacttttgc tataaatatc agaattttta tgaatgactc
    40801 attcaatttt tgaatataga aagatggata tggcatgtaa aggcaggagc cagcgctacg
    40861 cccgaggtgg catacagtct gtggcgtttt tctgacataa taggcaaatt cgattttgcc
    40921 tccgcaggag tgttttcatg gaatttacca ccggattgat gtcgctcgac accgcgctta
    40981 atgagatgct ttctcgcgtt accccactga ccgcccagga aacgctgcca ctggttcagt
    41041 gttttggtcg tattctggcg agcgatgtcg tttcgccact ggatgtcccg gggtttgata
    41101 actccgctat ggacggctac gcggtgcgtt tagccgatat tgcctccggg caaccgctgc
    41161 ccgttgccgg taaatccttt gccggtcagc cgtaccatgg tgaatggcct gcgggtacct
    41221 gcattcgtat tatgaccggt gcgccggtgc cagaaggctg cgaagcggtg gtgatgcagg
    41281 agcagactga acaaacggac aatggcgtgc gttttactgc tgaagtgcgt agcgggcaaa
    41341 atattcgccg tcgcggtgaa gatatctctg caggtgcggt tgttttcccg gcggggactc
    41401 gcctgactac cgctgaactg ccagtgattg cttcgctagg gattgccgaa gttccggtga
    41461 ttcgtaaagt gcgtgtagcg cttttttcta ccggtgatga actccagttg cccggtcagc
    41521 cgctgggcga cggccaaatc tacgatacca accgtctcgc cgtacacctg atgttagaac
    41581 agttgggatg cgaggtaatc aacttaggga ttatccgcga cgatccccat gccctgcgcg
    41641 ccgcatttat tgaagccgac agccaggcgg atgtagtgat cagttccggc ggtgtttcag
    41701 tgggtgaagc ggattacacc aaaacgattc ttgaagagct gggggagatc gccttctgga
    41761 aactggcgat taaaccgggt aaaccgttcg cgttcggtaa actttctaat agttggttct
    41821 gcggcctgcc gggcaacccg gtttcagcga cgctgacctt ctatcaactg gtacagcctt
    41881 tgctggcaaa actaagcggc aataccgcca gtggcctgcc cgcgcgccag cgtgtacgca
    41941 cagcgtcccg cctgaaaaaa acgccaggac gccttgattt ccagcgcggc gtgctgcaac
    42001 gcaacaccga tggcgaactg gaagtgacga ccaccggaca tcagggttca catatattta
    42061 gctcctttag cctcggcaac tgctttatcg tgctggaacg cgagcgcggc aatgttgacg
    42121 tgggcgaatg ggtggaagtc gaaccgttta acgcgttgtt cggaggcctg taatggcaga
    42181 actcagcgat caggagatgc tgcgctacaa ccggcaaatc attctgcgcg gttttgattt
    42241 tgacggtcag gaagcgctga aggattctcg cgtgttggtg gtggggcttg gtggcctcgg
    42301 ctgcgcagct tcgcagtatc tggcaagcgc cggtgtcggc aacctgacgc tgctcgactt
    42361 cgacacggtt tcgctctcca atctgcaacg ccagacgctg cacagtgatg ccacggtcgg
    42421 gcaaccaaag gtggaatccg cccgtgacgc cctgacgcgg atcaacccac atatcgcgat
    42481 tacgccagtc aatgcgctgc ttgatgacgc tgaacttgct gcgatgattg caaaacacga
    42541 tctggtgctc gactgtacgg ataacgttgc agtacgtaat caactgaacg caggctgttt
    42601 tgccgcgaag gtgccgctgg tttccggcgc ggcaattcgt atggaagggc aaatcacggt
    42661 cttcacttat caggacggcg aaccgtgcta tcgctgcctt agccgtttgt ttggcgagaa
    42721 cgcgttaacc tgcgtagaag caggcgtgat ggcaccgtta atcggcgtaa ttggttcgtt
    42781 gcaggcaatg gaagcaatca aactgctggc aggttatggc aaaccagcca gcgggaaaat
    42841 cgtgatgtac gatgcgatga cctgtcagtt tcgcgaaatg aaactgatgc gtaatccggg
    42901 gtgtgaggtg tgcgggcagt aactgccacc tccaggccag ataagacgcg gcatgaactg
    42961 caccccaaat gttggacata atctgacatc tgaaggtgca gtttatgaaa catt
//
LOCUS       DAZGJT010000031        41595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZGJT010000031 DAZGJT010000000
VERSION     DAZGJT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.031"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..821
                     /locus_tag="ACHKFK_001844"
     CDS             3..821
                     /locus_tag="ACHKFK_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001765455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO9052197.1"
                     /translation="MPCIGNAQEIKIDESWLHQSLNVIGRTDSRFGPRLTNDLYPEYT
                     VAGRKDWFDFYGYVDLPKFFGVGSHYDVGIWDEGSPLFTEIEPRFSIDKWTGLNLAFG
                     PFKEWFIANNYVYDMGDNQSSRQSTWYMGLGTDIDTGLPIKLSANIYAKYQWQNYGAA
                     NENEWDGYRFKIKYSIPLTNLFGGRLVYNGFTNFDFGSDLADKSQNNKRTSNAIASSH
                     ILSLLYEHWKFAFTLRYFHNGGQWNAGEKVNFGDGPFELKNTGWGTYTTIGYQF"
     gene            complement(1039..3471)
                     /gene="metL"
                     /locus_tag="ACHKFK_001845"
     CDS             complement(1039..3471)
                     /gene="metL"
                     /locus_tag="ACHKFK_001845"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO9052198.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(3474..4634)
                     /gene="metB"
                     /locus_tag="ACHKFK_001846"
     CDS             complement(3474..4634)
                     /gene="metB"
                     /locus_tag="ACHKFK_001846"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO9052199.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPREH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQSDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            4911..5228
                     /gene="metJ"
                     /locus_tag="ACHKFK_001847"
     CDS             4911..5228
                     /gene="metJ"
                     /locus_tag="ACHKFK_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO9052200.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            5288..5890
                     /gene="yiiX"
                     /locus_tag="ACHKFK_001848"
                     /pseudo
     CDS             5288..5890
                     /gene="yiiX"
                     /locus_tag="ACHKFK_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
     gene            complement(5943..6155)
                     /gene="rpmE"
                     /locus_tag="ACHKFK_001849"
     CDS             complement(5943..6155)
                     /gene="rpmE"
                     /locus_tag="ACHKFK_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO9052201.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            6358..8556
                     /gene="priA"
                     /locus_tag="ACHKFK_001850"
     CDS             6358..8556
                     /gene="priA"
                     /locus_tag="ACHKFK_001850"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO9052202.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQRKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRKNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQADNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILASPLADEKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            8712..9737
                     /gene="cytR"
                     /locus_tag="ACHKFK_001851"
     CDS             8712..9737
                     /gene="cytR"
                     /locus_tag="ACHKFK_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO9052203.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHNRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            9829..10788
                     /gene="ftsN"
                     /locus_tag="ACHKFK_001852"
     CDS             9829..10788
                     /gene="ftsN"
                     /locus_tag="ACHKFK_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO9052204.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASSQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            10881..11411
                     /gene="hslV"
                     /locus_tag="ACHKFK_001853"
     CDS             10881..11411
                     /gene="hslV"
                     /locus_tag="ACHKFK_001853"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO9052205.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            11421..12752
                     /gene="hslU"
                     /locus_tag="ACHKFK_001854"
     CDS             11421..12752
                     /gene="hslU"
                     /locus_tag="ACHKFK_001854"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO9052206.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            12819..13745
                     /gene="menA"
                     /locus_tag="ACHKFK_001855"
     CDS             12819..13745
                     /gene="menA"
                     /locus_tag="ACHKFK_001855"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO9052207.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGAVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            13838..14323
                     /gene="rraA"
                     /locus_tag="ACHKFK_001856"
     CDS             13838..14323
                     /gene="rraA"
                     /locus_tag="ACHKFK_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO9052208.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGASGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(14408..14653)
                     /gene="zapB"
                     /locus_tag="ACHKFK_001857"
     CDS             complement(14408..14653)
                     /gene="zapB"
                     /locus_tag="ACHKFK_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO9052209.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            15078..15923
                     /gene="glpF"
                     /locus_tag="ACHKFK_001858"
     CDS             15078..15923
                     /gene="glpF"
                     /locus_tag="ACHKFK_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO9052210.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            15946..17454
                     /gene="glpK"
                     /locus_tag="ACHKFK_001859"
     CDS             15946..17454
                     /gene="glpK"
                     /locus_tag="ACHKFK_001859"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO9052211.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            17625..18635
                     /gene="glpX"
                     /locus_tag="ACHKFK_001860"
     CDS             17625..18635
                     /gene="glpX"
                     /locus_tag="ACHKFK_001860"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO9052212.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            18732..19478
                     /gene="fpr"
                     /locus_tag="ACHKFK_001861"
     CDS             18732..19478
                     /gene="fpr"
                     /locus_tag="ACHKFK_001861"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO9052213.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAVGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(19483..19911)
                     /gene="uspD"
                     /locus_tag="ACHKFK_001862"
     CDS             complement(19483..19911)
                     /gene="uspD"
                     /locus_tag="ACHKFK_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO9052214.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKLSADLLIVPFIDK"
     gene            complement(19938..20237)
                     /gene="yiiS"
                     /locus_tag="ACHKFK_001863"
     CDS             complement(19938..20237)
                     /gene="yiiS"
                     /locus_tag="ACHKFK_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO9052215.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(20449..20889)
                     /gene="yiiR"
                     /locus_tag="ACHKFK_001864"
     CDS             complement(20449..20889)
                     /gene="yiiR"
                     /locus_tag="ACHKFK_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO9052216.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFIPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            20990..21589
                     /gene="yiiQ"
                     /locus_tag="ACHKFK_001865"
     CDS             20990..21589
                     /gene="yiiQ"
                     /locus_tag="ACHKFK_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1454 family protein"
                     /protein_id="HFO9052217.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQITWLPIQGPEQKAAKAKAQAYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            21697..22464
                     /gene="tpiA"
                     /locus_tag="ACHKFK_001866"
     CDS             21697..22464
                     /gene="tpiA"
                     /locus_tag="ACHKFK_001866"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO9052218.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(22519..23274)
                     /gene="cdh"
                     /locus_tag="ACHKFK_001867"
     CDS             complement(22519..23274)
                     /gene="cdh"
                     /locus_tag="ACHKFK_001867"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO9052219.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEQCLP
                     NQQENQNPSPCAEVKPNAGYVVLKDRHGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSQKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(23381..24370)
                     /gene="sbp"
                     /locus_tag="ACHKFK_001868"
     CDS             complement(23381..24370)
                     /gene="sbp"
                     /locus_tag="ACHKFK_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO9052220.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(24690..25652)
                     /gene="pfkA"
                     /locus_tag="ACHKFK_001869"
     CDS             complement(24690..25652)
                     /gene="pfkA"
                     /locus_tag="ACHKFK_001869"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO9052221.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(25833..26735)
                     /gene="fieF"
                     /locus_tag="ACHKFK_001870"
     CDS             complement(25833..26735)
                     /gene="fieF"
                     /locus_tag="ACHKFK_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO9052222.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(26884..27384)
                     /gene="cpxP"
                     /locus_tag="ACHKFK_001871"
     CDS             complement(26884..27384)
                     /gene="cpxP"
                     /locus_tag="ACHKFK_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO9052223.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEIAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            27534..28232
                     /gene="cpxR"
                     /locus_tag="ACHKFK_001872"
     CDS             27534..28232
                     /gene="cpxR"
                     /locus_tag="ACHKFK_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO9052224.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALSLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            28229..29602
                     /gene="cpxA"
                     /locus_tag="ACHKFK_001873"
     CDS             28229..29602
                     /gene="cpxA"
                     /locus_tag="ACHKFK_001873"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO9052225.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(29773..30447)
                     /gene="yiiM"
                     /locus_tag="ACHKFK_001874"
     CDS             complement(29773..30447)
                     /gene="yiiM"
                     /locus_tag="ACHKFK_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO9052226.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTDLGLEGDEQ
                     AEKKIHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYIGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGLVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWEK"
     gene            complement(30596..31578)
                     /gene="kdgT"
                     /locus_tag="ACHKFK_001875"
                     /pseudo
     CDS             complement(30596..31578)
                     /gene="kdgT"
                     /locus_tag="ACHKFK_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(31838..32458)
                     /gene="sodA"
                     /locus_tag="ACHKFK_001876"
     CDS             complement(31838..32458)
                     /gene="sodA"
                     /locus_tag="ACHKFK_001876"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO9052227.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     NLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVQKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            32744..33778
                     /gene="rhaT"
                     /locus_tag="ACHKFK_001877"
     CDS             32744..33778
                     /gene="rhaT"
                     /locus_tag="ACHKFK_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO9052228.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGSGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(33775..34623)
                     /gene="rhaR"
                     /locus_tag="ACHKFK_001878"
     CDS             complement(33775..34623)
                     /gene="rhaR"
                     /locus_tag="ACHKFK_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO9052229.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDETSCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(34697..35533)
                     /gene="rhaS"
                     /locus_tag="ACHKFK_001879"
     CDS             complement(34697..35533)
                     /gene="rhaS"
                     /locus_tag="ACHKFK_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO9052230.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQEQDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            35821..37290
                     /gene="rhaB"
                     /locus_tag="ACHKFK_001880"
     CDS             35821..37290
                     /gene="rhaB"
                     /locus_tag="ACHKFK_001880"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO9052231.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQHGQRVGLPVAY
                     RDSRTHGLMAQAQQQLGKRDIYQRSGIQFLPFNTIYQLRALTEQQPELIPHIAHALLI
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            37287..38546
                     /gene="rhaA"
                     /locus_tag="ACHKFK_001881"
     CDS             37287..38546
                     /gene="rhaA"
                     /locus_tag="ACHKFK_001881"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO9052232.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGLLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQITPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDRIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLENVRTYEKEILSRRG"
     gene            38731..39555
                     /gene="rhaD"
                     /locus_tag="ACHKFK_001882"
     CDS             38731..39555
                     /gene="rhaD"
                     /locus_tag="ACHKFK_001882"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO9052233.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADITP
                     YHDNFHQQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGIVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     PTLDETFGLIDTAEKSAQILVKVYSMGGMKQTISREELIALGQRFGVTPLASALAL"
     gene            39565..39879
                     /gene="rhaM"
                     /locus_tag="ACHKFK_001883"
     CDS             39565..39879
                     /gene="rhaM"
                     /locus_tag="ACHKFK_001883"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO9052234.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKAHNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANADNSPVSSELQEVFY
                     LP"
     gene            complement(39920..41314)
                     /locus_tag="ACHKFK_001884"
     CDS             complement(39920..41314)
                     /locus_tag="ACHKFK_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO9052235.1"
                     /translation="MKKSTLSLAIGLLLACSTGMAKTQHLTLEQRMALLEERLEAAEM
                     RAAKAEGQVKQLQTQQAAEIREIKTAQGNTPVNGQSTTESEKKNATPPNLLLSGYGDL
                     KIYGDVEFNMDAESNHGLLAMTNADVNSDPTNEQWNLNGRILLGFDGMRKLDNGYFAG
                     FSAQPLGDMHGSVNIDDAVFFFGKENDWKVKVGRFEAYDMFPLNQDTFVEHSGNTAND
                     LYDDGSGYIYMMKEGRGRSNAGGNFLVSKQLDNWYFELNTLLEDGTSLYNDGNYHGRD
                     MEQQKNVAYLRPVIAWSPTEEFTVSAAMEANVVNNAYGYTDSKGNFVDQSDRTGYGMS
                     MTWNGLKTDPENGIVVNLNTAYLDANNEKDFTAGINALWKRFELGYIYAHNKIDEFSG
                     VVCDNDCWIDDEGTYNIHTIHASYQFANVMDMENFNIYLGTYYSILDSDGDKIHGDDS
                     DDRYGARVRFKYFF"
BASE COUNT        10273 a        10857 c        10608 g         9857 t
ORIGIN      
        1 taataccttg cattggaaac gctcaggaaa ttaaaattga tgaaagctgg ttacatcaaa
       61 gcttgaatgt cattggtcgc acagactctc gctttggccc aagactgact aacgacctct
      121 accctgaata tactgtagca ggaagaaaag actggtttga tttttatggt tatgttgatc
      181 taccgaaatt ctttggcgtc ggcagtcact atgatgttgg gatctgggat gagggctcac
      241 cactatttac ggaaatagaa cctcggtttt ccattgacaa atggaccgga ttaaatcttg
      301 cgttcggccc atttaaagaa tggttcattg caaacaacta tgtctatgat atgggtgaca
      361 accagtcatc ccggcaaagt acatggtata tggggcttgg tacagatatc gacacgggtc
      421 taccaattaa gctttctgcc aatatatacg ccaagtatca gtggcaaaac tatggtgccg
      481 ctaatgaaaa tgaatgggac ggatatcgat tcaaaataaa atatagcatc cctcttacaa
      541 atttattcgg agggcgattg gtatacaatg gttttactaa ctttgatttt ggctccgatc
      601 ttgcggacaa gtcacaaaat aataaacgaa ccagtaatgc tattgcttca agccatatcc
      661 tttcccttct atatgaacac tggaaatttg catttacact acgttatttt cacaacggtg
      721 gacaatggaa tgcgggagag aaggttaact tcggagatgg tccatttgaa ttaaaaaata
      781 caggatgggg aacctatact actattggtt atcaatttta aattaggtga cattttagat
      841 attgttactt aatgtcattt tgtaattgtc attaataact tcaccattca ctctgttact
      901 tcatcgctca acacaaaagc ccccttatta ccgggggcac tgaccaaagg caaaatgaca
      961 atcaatcacc acttccccag gaagaggttt aggggtgaca acaaaagctg gcaactgcca
     1021 gcttttgttc aataatcact acaacaactg cgccagtcgg ttaatatccg actgaatcgc
     1081 cccggcggtg acgtcgcgcc ctgcacctgg cccgcggatc accagcgggt tgtcgcgata
     1141 ccagcggctt tcgatggcaa agacgttatc gcatggcagt agtgatgcca gcggatggtc
     1201 ttcacgcacc gcttccacgc ccacacgcgc tttgccgttg gcatcgaaac gcgccacgta
     1261 acgcagcacc agccccattt cgcgggccgc ttccagtcgt tgcaccatct gctcgttaag
     1321 ttcatcgcca ttttcaaaga aatggtcgat gctgccgcct tcgcaatggg caggcaccag
     1381 tgattcaaca cgtacctggt ccggttcaat gttgtaaccc gcttcacgcg ccagaatcac
     1441 cagcttgcgc atcacgtctt tgccggaaag gtcatcacgc gggtcaggct cggttaagcc
     1501 ctgctgccac gcctgatcca ccagttcggt aaacggcacg ctaccgtcga attgcaggaa
     1561 cagccaggag agcgtgccgg agaagatccc actgatcgac aaaatggtat cgccgctgtc
     1621 gatcagatcg cgcacggtat ggttgatcgg caaacccgca ccgacagtgg cattgtacag
     1681 ccagtgacgc ccggtttttt cgaaggcgtc gtggatctgg cgatatttat tgctgtcgct
     1741 cgctcctgcc agtttgttgg cactgatgac gtggaaaccg tggctggcga aatcaagata
     1801 ctggtcagcg agctgctggc tggcggtaac gtccagcacc actaaatcat catacggatg
     1861 ggcgcgcatc cacaggaaca gcgactcttc atcctgctcg actgcttcat cgttgaagaa
     1921 ggctaacgcg cggctggcgt ccagaccgtc atagctcaac aggctacggc ggctgtccac
     1981 cacgcctgcc agcacaaatt caaagccggt acgtgccgaa agcgtactct gctcacgggc
     2041 gaacagttcc agccagcggg aaccgatatt gcccttgccg aacaacacca ggccgatgcg
     2101 tttttccgca cggaagacgg actgatgcag cccctgaatc aggctttcgg tcgggccggt
     2161 gcgcagtact gccaccaggc tgatgccatc atcagactgc caggtaaact cgaccggctg
     2221 gcctttcagt tgctgccaga agcggtggca atgcagcggg ttacgggtga cgcccgcccc
     2281 aaccatcgcc accagcgcca gcccctgacg caggcgcagt tcgccaggta atcccgcttc
     2341 gtcgaggatt ttcagcgcac tgtcggccac ttctgaggtg tagcaaaatt gcagcaactg
     2401 gcgatcgtta tgcacgccaa ccgccagtgg gcgcacctga gcacgtttca ggatctggtc
     2461 gatctcttta tgcgccagtt tgaaatcctg gctggcgggc acctgaaact caatcaagca
     2521 gacatcatca tggctggtga caatacgcgc accagtaccg gaggccagca cgcgttcaat
     2581 gcgcgtggaa ccttgatccg gcgtgtagct acagcgcagt tgcaggtcga tttcgctgcc
     2641 agaaacgggc tgtaaagtac gggcatgaag aacgggagcc gccagacgcg ccagttcgct
     2701 ggcttcatcc agacgcagca acggcagcag gcaggcatct ttcactttac gcgggtcggc
     2761 gctatatacc ccggcgacgt cgctccagat ggttacgcga gaaacacccg ccagcgcacc
     2821 gatttgtgtc gcggaatagt cggaaccgtt acgccccagc agcaccgttt caccggcgtt
     2881 gttacggctg ataaacccgg tcaccaccag acgtttgccc ggatgttgta ccagtagctg
     2941 ttgcagcaac gggtaagaaa gtccttcatc aacctgcggt tgcgcagcgc gttcagcgcg
     3001 taaaaactcg cgggcatcaa gccaggccgc tggcagccct tgttgattaa gtaccgcaga
     3061 catcaggcgc gccgaccaca cttcaccgtg gcccaccact tctgcataca ccgcgtcgtt
     3121 aataccgctg tcgagcagtg ccgccaggcg ttccagatcg ctgacaaaag cgctaatgag
     3181 gctatctgct tcttcagcgg gtagcaggcc gctaatcaga tcgcactgat agcgacgtag
     3241 cgtttgttga acctgatgcg cagagagacg atcggtctgg cttagtttca accagttaat
     3301 caactggtta gtggtgctac cggcggcgga aaccaccatc atatcgtcag gctgagagta
     3361 ctccgccata atgcccgcga cacgcaaata acacttcaca tcagccagac tactgccacc
     3421 aaatttatgc agttgacgac ctttcgcccc tgcctgcgca atcacactca tttttacccc
     3481 ttgtttgcag cccggaagcc attttccagg tcggcaatta aatcttcgcc atcttcaata
     3541 ccggtggaga tacgcagcag cgtctcggag atcccggcgg cagcacgcgc ttctggtgcc
     3601 atgcctgcat gtgtcatggt tgcggcgtga gagattaaac tttccactcc ccctaatgat
     3661 tccgccagcg taaacaacga cagcccaccc aggaaacgac gcagcgtctg ctcatcgcca
     3721 tccagttcaa aactcaacat tgcgccaaag cctttttgct ggcgcgcggc aatttcatgc
     3781 ccctgatttt ccggcagcga cgggtgatac agttttttca ccaacggctg ggtttgcaga
     3841 tatttcacaa tcgcctgcgc gttgcgctgg gccagctcca ttcgcggcac cagcgttcgc
     3901 aacccgcgca gcagcagata gctgtcaaac gcgccgcccg tcacgccaat attgtttgcc
     3961 caccaggcaa gttcagtgac aacgtccggg tctttagcaa tcactacgcc tgccaccacg
     4021 tcagagtgac cgttcagata tttcgtgcat gaatgcaaca ccagatcggc acctaatgcc
     4081 agtggatttt gtaatgccgg gcttaagaag gtgttatcca ccacgctcac cgccccgact
     4141 tcccttgcca gatggcagat tttcgcaata tccacgacgc gtaacaatgg attacttggg
     4201 ctttctacca gtaccagttt gggtttttct gccagcgctg cccgtaatgc ctgttcatcg
     4261 ctttgatcaa caaacaacac gcgatagcaa ccgcgtttcg ccaggctgtc gaacaggcga
     4321 tagctaccgc cgtagcagtc gtgcggcgca accagcagat cgccaggttt caaaaagacg
     4381 gtcgttacca ggtgaatcgc ggacatgccg gtattagtaa gtactgcacc agcaccgcct
     4441 tccagttctg ccagcgctcg ctgaaccaca tcgcgcgttg ggttgccgcg ccgcgagtaa
     4501 tcatgctcgc gcggttcatt aaatccggta aagttatagg tactggaaag atggatggga
     4561 gggacaacgc aaccatactg ttcgtcgtca tttaacccgc tacgcactgc gatggtggcc
     4621 tgtttacgcg tcatgtgatg aagttccctg ggcttggtcg gtgaaatgtc aggcaccaga
     4681 gtaaacattg tgttaatgga cgtcaataca tctggacatc taaacttctt tgcgtataga
     4741 ttgagcaaat ctcaaatagc cgttaaaatt atatgcatta tcacgccgac aggtgcatta
     4801 cacgatgtca cggtaacgcc tgtacggtaa actatgcggg tttacggtca gtacccatat
     4861 taattgtgtg gtctggcctc aatttattga cgaagaggat taagtatctc atggctgaat
     4921 ggagcggcga atatatcagc ccatacgctg agcacggcaa gaagagtgaa caagtcaaaa
     4981 aaattacggt ttccattcct cttaaggtgt taaaaatcct caccgatgaa cgcacgcgtc
     5041 gtcaggtgaa caacctgcgt cacgcgacca acagcgagct gctgtgcgaa gcgtttctgc
     5101 atgcctttac cgggcaacct ttgccggatg atgccgacct gcgtaaagag cgcagcgacg
     5161 aaattccgga ggcggcaaaa gagatcatgc gtgagatggg gattaacccg gagacgtggg
     5221 aatactaaag cggaaaagag cggcgcggag ttaaccagcc gcttttttca tacatggacg
     5281 tttaacgatg aaaatcaggc tactgattct cagcctgctg gtttctgtgc ctgcatttgc
     5341 ctggcaacca caaaccggcg acatcatctt tcagatctct cgctcatcgc aaagtaaagc
     5401 gatccaactg gcgacccatt ccgattacag ccacaccggt atgctggtga tacgcaacaa
     5461 aaagccctac gtttttgaag cggtcggccc ggtaaaatac accccactca agcagtggat
     5521 cgcccatggt gaaaagggca aatacgttgt tcgccgcgtt gaaggcggac tgagtgttga
     5581 acaacagcaa aaactggcgc aaacggcaaa acgttatctc ggtaaaccgt atgactccag
     5641 cttctcgtgg agcgacgatc gccagtactg ttcggaagtg gtgtggaagg tctaccagaa
     5701 tgcgctggga atacgtgtgg gcgaacagca gaagctgaaa gagttcgatc ttagtaaccc
     5761 gctggttcag gcaaagctca aagaacgcta cggcaaaaat attccgcttg aggaaacggt
     5821 agtttcacca caagccgttt tcgacgcgcc acaactcacc acggtcgcca aagaatggcc
     5881 gctgttttca aaattcaggc acaaaaaaag cgccgtgcgg cgcttttttc ggaaatccgg
     5941 tcttatttgc tgcccgggat gttgaaacgc ttgttgaagc ggtcaacacg gccaccggta
     6001 gcaacatcac gctgtttgcc agtgaagaac gggtggcact tgctgcacac gtcgaggttc
     6061 aggtcatgac caacggtgga gcggattttc attacgttac cgcaagagca gctagcagta
     6121 atttcttcgt atttcgggtg aatatctttt ttcatgggaa aacctcggtt taaggccgcg
     6181 tcgctcttcc agccctaacg ccagacacca cgcgatgtta aaagtatagc ttcaatacga
     6241 tcatttcgta cgaaggcgcg aaatcataca gaaattaacc agcgtatgca aactgatccg
     6301 cactcttcta cggcaatgtg tatactaacc caccgaattt caagtcagga tgatgctatg
     6361 cccgttgccc acgttgcctt gcccgttccg cttcctcgta cctttgacta tctgctgcca
     6421 gaaggcatga cggttaaagc cgggtgtcgc gtgcgcgtgc cgtttggcaa acagcaggag
     6481 cgcatcggga ttgtggtatc agttagcgat gccagcgaac tgccgctcaa tgagctaaaa
     6541 gcggtagtcg aagtgctgga cgttgagccg gtatttactc actccgtctg gcgattactg
     6601 ctctgggcgg cagattacta tcatcatccg attggcgatg tgctgtttca tgccttaccg
     6661 attttactgc gccaggggcg gcctgcggcg aacgcgccga tgtggtactg gtttgccact
     6721 gaacaaggtc aggcggtgga tctgaacagc ctgaaacgtt cgccaaagca acaacaggcg
     6781 ctggcagcgt tacggcaacg caaaatctgg cgtgatcagg tcgcggaact tgaatttaat
     6841 gatgccgcgt tgcaggcgct gcgcaaaaaa ggtctgtgtg atttagcaag tgaaacacca
     6901 gagtttagcg actggcgaaa gaattatgcc gtttctggtg agcggttgcg attgaatacc
     6961 gaacaggcca ccgccgttgg cgcaattcat agcgcggcag atactttttc tgcctggctg
     7021 ctggcgggcg ttaccggttc cggtaaaacg gaggtttatc tcagcgtact ggaaaacgtg
     7081 ctcgctcagg gcaaacaggc gctggtgatg gtgccggaaa tcggcctgac accgcaaact
     7141 atcgcccgtt ttcgtgaacg ttttaatgcc cccgtggaag ttctgcattc cggcctgaac
     7201 gacagcgagc gtctttcggc gtggttgaaa gcgaaaaatg gcgaggcggc gattgtgatc
     7261 ggcacccgct ccgcgctgtt tacaccgttt aaaaatctcg gcgtgattgt cattgatgaa
     7321 gagcacgaca gttcctataa acagcaggaa ggctggcgct accatgcccg cgacctggcg
     7381 gtgtatcgtg cgcatagcga gcaaatccca attattcttg gctctgcaac gcccgcactg
     7441 gaaacgttat gcaacgtgca gcagaaaaaa taccgcctgc tgcgcctgac ccgtcgggcg
     7501 ggcaatgcgc gcccggcaat tcaacatgtg ctggatttaa aaggtcagaa ggtgcaggca
     7561 ggtctggctc cggcgttaat cacccgtatg cgccagcatt tacaggccga caaccaggtt
     7621 atcctctttc ttaaccgccg gggttttgcg cctgcgctac tgtgccacga ctgtggttgg
     7681 atagccgaat gcccacgttg cgatcactac tacacgctgc atcaggcgca acagcatttg
     7741 cgctgccacc attgcgacag tcagcgtccg gtgccgcgcc agtgcccttc ttgcggttcc
     7801 acgcacctgg tacccgtggg gctgggcacc gaacagcttg aacagacgct cgcgccgttg
     7861 ttccctggtg tccccatttc tcgtatcgac cgcgatacca ccagccgcaa aggcgcgctg
     7921 gaacaacaac tggcggaagt acatcgcggt ggcgcacgga tattgatcgg tacacaaatg
     7981 ctggcgaaag gtcaccactt tccggatgtg acgctggtgg cgttactgga tgtggacggc
     8041 gcgctgtttt ctgcggattt tcgctcggca gagcgttttg cccagcttta tacccaggtg
     8101 gcaggccgtg ccgggcgtgc gggtaaacag ggcgaagtag ttctgcaaac gcaccatcct
     8161 gaacatccgc tgttgcaaac gttgctttat aaaggctacg acgcctttgc cgaacaggca
     8221 ctggctgaac gacgcatgat gcagctgccg ccgtggacca gccatgtgat tgtgcgtgcg
     8281 gaagatcata acaatcagca cgcgccattg ttcctgcaac aactgcgtaa tctcattctc
     8341 gccagcccac tggcagacga gaaactgtgg gttctcggtc cggttccggc gctggcccct
     8401 aaacgtggcg gtcgctggcg ctggcagata ttgttgcagc acccttcccg cgtgcgctta
     8461 cagcacatca tcaatggtac gctggcgctt attaatacaa taccggattc ccgcaaggtg
     8521 aaatgggtgc tggatgtcga tccgattgag ggttaaaccg ctcacgatgc gaggcggatc
     8581 gaaaaattca atattcatca cacttttcat gaaaattctg taaccgtttt cacgcgctat
     8641 ctgctaaaaa tgttgccgat gtgaagtaaa catggatgta gtacgcctga cgtgccaggc
     8701 gaggagtgag tgtgaaagcg aagaagcagg aaactgccgc gaccatgaaa gacgttgccc
     8761 tcaaggcaaa agtctctaca gcgaccgtct cccgagcatt aatgaatccc gataaagtct
     8821 cccaggccac tcgtaatcgg gttgaaaaag cggcccggga agtgggttat ttaccacagc
     8881 ctatggggcg caacgtcaag cgtaatgaat cccgtaccat tctggtgatt gtcccggata
     8941 tctgcgatcc cttctttagc gaaattattc gcggtatcga agttacggcg gcaaatcacg
     9001 gatatctggt gctgattggc gactgtgcgc atcaaaatca gcaggaaaaa acctttatcg
     9061 atttgatcat caccaagcaa attgatggca tgttgttgct gggttcaagg ctgccgtttg
     9121 atgccagcat tgaggaacag cgtaatctgc cgccgatggt gatggcgaac gaatttgcac
     9181 cggaactgga gctgcctacc gttcatatcg acaatctgac cgccgcattt gatgcagtaa
     9241 attatttata tgagcaaggg cataatcgga ttggctgtat agccggtccc gaagagatgc
     9301 cgctgtgtca ctaccgcctg caaggctatg tccaggcgct gcgtcgctgc ggcattatgg
     9361 ttgatccgca atacatcgcc cgtggcgact tcaccttcga agccggaagc aaagcgatgc
     9421 agcagttgct tgatcttcca caaccgccta ctgctgtctt ctgccatagc gatgtgatgg
     9481 cgctcggcgc actttctcag gcaaaacgcc aggggctgaa agtcccggaa gacctttcca
     9541 taatcggttt tgataacatc gacctgacgc aattttgtga tccgccgctg acaaccatcg
     9601 cgcagccgcg ttacgaaatc ggtcgggaag ctatgctgtt attgcttgat caaatgcagg
     9661 ggcaacacgt tggcagtggc tctcgtttaa tggactgcga acttatcatc cggggatcaa
     9721 cacgcgcgtt accttaaagt aaacctttaa gactgccgtg actggtcaaa ggcccgccgc
     9781 ttaagtaaca tggcgggctg acgaacgaat aaatacagcg aaacgatagt ggcacaacga
     9841 gattatgtac gccgcagcca accggcacct tcgcggcgaa aaaagagcac ctcacggaaa
     9901 aagcaacgaa atctgcctgc ggtttctccc gctatggtcg ctattgctgc cgccgttctt
     9961 gtgaccttta tcggtggtct gtacttcatt acgcatcaca agaaagaaga gtccgagacg
    10021 ctgcaaagcc agaaagtgac cggaaacgga ctaccaccaa aaccagaaga acgctggcgc
    10081 tacattaaag agctggaaag tcgccagccg ggtgtgcgtg cgcccacaga accttctgcc
    10141 ggtggtgaag tgaaaacgcc ggagcaactg acaccagaac aacgccagct tcttgaacaa
    10201 atgcaggccg atatgcgcca gcagccaacg cagctggttg aagttccgtg gaatgagcag
    10261 acgccggaac agcgtcagca aacgctacag cgccagcgtc aggcgcagca attagcggag
    10321 cagcaacgcc tggctcagca gtcgcgtacg actgaacaaa gctggcagca gcagacgcgt
    10381 acgtcgcaag ccgcaccggt acaggcgcaa ccgcgccagt ctaaaccggc ttcttctcag
    10441 cagccgtacc aggatctgct gcaaactcct gcgcacacga ctgcgcagtc gaagccgcag
    10501 caagctgccc cggttgctcg tgccgctgac gcgccaaaac cgacggcgga gaaaaaagac
    10561 gaacgccgct ggatggtgca gtgcggttcg ttcagaggcg cggaacaggc agagacggta
    10621 cgtgctcagc tggcgttcga aggctttgac tcgaaaatca ccaccaacaa tggctggaat
    10681 cgtgtggtca ttggcccggt gaaaggcaaa gagaacgcag acagcaccct caatcgcttg
    10741 aagatggcgg gtcatacaaa ctgcattcgg ctcgccgccg ggggttgaaa ccctcaaaat
    10801 cccccccatc tataattgca ttatgccccg tacttttgta cggggtttgt actctgtatt
    10861 cgtaaccaag gggtcagctc gtgacaacta tagtaagcgt acgccgtaac ggccatgtgg
    10921 tcatcgctgg tgatggtcag gccacgttgg gcaataccgt aatgaaaggc aacgtgaaaa
    10981 aggtccgccg tctgtacaac gacaaagtca tcgcgggctt tgcgggcggt actgcggatg
    11041 cttttacgct gttcgaactg tttgaacgca aactggaaat gcatcagggc catctggtca
    11101 aagccgccgt tgagctggca aaagactggc gtaccgatcg catgctgcgc aaacttgaag
    11161 cactgctggc agtcgcggat gaaactgcat cgcttatcat caccggtaac ggtgacgtgg
    11221 tgcagccaga aaacgatctt attgctatcg gctccggcgg cccttacgcc caggctgcgg
    11281 cgcgcgcgct gttagaaaac actgaactta gcgcccgtga aattgctgaa aaggcgttgg
    11341 atattgcagg cgacatttgc atctatacca accatttcca caccatcgaa gaattaagct
    11401 acaaagcgta aggatctccc atgtctgaaa tgaccccacg cgaaatcgtc agcgaactgg
    11461 ataagcacat catcggccag gacaacgcca agcgttctgt ggcgattgct ctgcgtaacc
    11521 gctggcgtcg catgcagctc aacgaagagc tgcgccatga agtgaccccg aaaaatatcc
    11581 tgatgatcgg cccgaccggt gtcggtaaaa ctgaaatcgc ccgtcgtctg gctaagctgg
    11641 cgaatgcgcc gttcatcaaa gttgaagcga ccaaattcac cgaagtgggc tatgtcggta
    11701 aggaagtgga ttctattatt cgcgatctga ccgatgccgc cgtgaaaatg gtacgcgtcc
    11761 aggctatcga gaaaaaccgt tatcgcgctg aagaactggc agaagaacgt attctcgacg
    11821 tgctgatccc acctgctaaa aacaactggg gacagaccga acagcagcag gaaccgtccg
    11881 ctgctcgtca ggcattccgc aagaaactgc gtgaaggcca gcttgatgac aaagaaatcg
    11941 agatcgatct tgccgcagca ccgatgggcg ttgaaattat ggctcctccg ggaatggaag
    12001 agatgaccag ccagctgcag tccatgttcc agaacctggg cggccagaag caaaaagcac
    12061 gtaagctgaa aatcaaagac gccatgaagc tgctgattga agaagaagcg gcgaaactgg
    12121 tgaatccgga agagctgaag caagacgcta tcgacgctgt tgagcagcac gggatcgtgt
    12181 ttatcgacga aatcgacaaa atctgtaagc gcggcgagtc ttccggtccg gatgtttctc
    12241 gtgaaggtgt tcagcgtgac ctgctgccgc tggtagaagg ttgcaccgtt tccaccaaac
    12301 acgggatggt caaaactgac cacattctgt ttatcgcttc tggcgcgttc cagattgcga
    12361 aaccgtctga cctgatcccg gaactgcaag gtcgtctgcc aatccgcgtt gagctgcagg
    12421 cactgaccac cagcgacttc gagcgtattc tgaccgagcc gaatgcctct atcaccgtgc
    12481 agtacaaagc actgatggcg actgaaggcg taaatatcga gtttaccgac tccggtatta
    12541 agcgcatcgc ggaagcggca tggcaggtga acgaatctac cgaaaacatc ggtgctcgtc
    12601 gtttacatac cgttctggag cgtttaatgg aagagatttc ctacgacgcc agcgatttaa
    12661 gcggtcaaac tatcatcatt gacgcagatt atgtgagcaa acatctggat gcgttggtgg
    12721 cagatgaaga tctgagccgt tttatcctat aatcgcgttc aatcattttc atcattgttt
    12781 gatggggctg aaaggcccca tttttattgg cgcgtattat gactgaacaa caaattagcc
    12841 gaactcaggc gtggctggaa agtttacgac ctaaaaccct ccccctcgcc tttgctgcaa
    12901 ttatcgtcgg gacggcgctg gcatggtggc aaggtcactt cgatccgctg gtcgccctgc
    12961 tggcactgat taccgccggg ctattacaga tcctttctaa cctcgccaat gattacggcg
    13021 atgcggtaaa aggcagcgat aaacctgacc gtatcggacc gctacgcggc atgcaaaaag
    13081 gggtcattac ccagcaagag atgaaacggg cgctcattat taccgtcgtg ctcatctgtc
    13141 tctccgggct ggcactggtt gcagtggcgt gccatacact ggccgatttt gtcggcttcc
    13201 tgattcttgg cgggttgtcg atcattgccg ctatcaccta caccgtgggc aatcgtcctt
    13261 atggttatat cggtctgggt gatatttccg tactggtttt ctttggctgg ttgagcgtca
    13321 tggggagctg gtatttacag gctcatacat tgattccggc actgatcctt ccggcgaccg
    13381 catgcggcct gctggcaacg gcagtactga acattaataa cctgcgtgat atcaatagcg
    13441 accgcgaaaa cggcaaaaac acgctggtgg tgcgcttagg tgcagtaaac gcgcgtcgtt
    13501 atcatgcctg cctgctgatg ggctcactgg tgtgtctggc gctgtttaat ctcttttcgc
    13561 tgcatagcct gtggggctgg ctgttcctgc tggcggcacc gttgctggtg aagcaagctc
    13621 gttatgtgat gcgcgaaatg gacccggtgg cgatgcgacc aatgctggaa cgcactgtca
    13681 aaggagcgtt actgactaac ctgctgtttg ttttagggat attcctaagc cagtgggcag
    13741 cataactgac aaatatcaat taacaattga tgattttgcc aacagcccac atagcgcgat
    13801 atactgaaaa ttctcgcagc aactgaatgt taagcctatg aaatacgata cttccgagct
    13861 ttgtgacatc tatcaagaag atgttaacgt cgtggaaccg ctgttctcca actttggcgg
    13921 acgagcgtcg tttggcggac aaataatcac ggtaaaatgt ttcgaggaca acgggttgct
    13981 gtacgatctg ctcgaacaga atggccgtgg tcgtgttctg gtcgtcgatg gcggtggttc
    14041 tgttcgtcgc gcactggtcg atgctgaact ggcgcgtctg gcagtacaaa atgaatggga
    14101 aggtctggtc atttacggcg cggtgcgtca ggtagatgac ctggaagagc tggatatcgg
    14161 catccaggcg atggcggcaa ttccggttgg tgcctctggc gaaggcattg gcgaaagcga
    14221 tgtccgcgtc aattttggcg gtgtcacctt cttctccggc gaccatcttt atgccgacaa
    14281 taccgggatt attctttcag aagatccgtt ggatattgaa tgatagaaaa ggcaccgcaa
    14341 ggtgcctttt ttctgcgtta cctgttggcc tacacagtaa agaaattacg cggaagatga
    14401 agcgtaatca gacctcttcc atgcgaccca gcagggcctg cagacgttcc tgccagccgt
    14461 tctgctgttc tttcagatgg ttgttctcac gctccagctc ttcgcgctga tgctgggcat
    14521 tttgaacttc ctgcgacagt gagttgtttt tttctttcag ctcttcgatt tccatctgca
    14581 acagagtgat ggtatcaatc gcctgctgta cttttgcttc cagtttctca aacacttcta
    14641 atgacattgt catacctctc ctgaattgca aggcgttgat ggataaaaat cctcgtcccg
    14701 attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat tgtatgaagc
    14761 cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac ttttcatcct
    14821 tctcctggtg acataatcca catcaatcga aaatgttaat aaatttgttg cgcgaatgat
    14881 ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac gctcatttta
    14941 tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta acgatgagtt
    15001 tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa caaacattaa
    15061 ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc attgctgaat
    15121 tcctcggtac cgggttgttg atttttttcg gggtgggttg cgttgcagca ctaaaagtcg
    15181 ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg gtggcaatgg
    15241 ccatctacct gaccgcaggg gtttccggcg cgcatcttaa ccccgctgtt accattgcat
    15301 tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt tcacaagttg
    15361 ccggcgcttt ctgcgctgcg gctttagttt acgggcttta ctacaattta tttttcgact
    15421 tcgagcagac tcatcatatt gttcgcggca gcgttgaaag tgttgatctg gctggcactt
    15481 tctctactta cccgaatcct catatcaatt ttgtgcaggc tttcgcagtt gagatggtga
    15541 ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac ggtgtaccac
    15601 gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc gcatctatgg
    15661 gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa gtctttgcct
    15721 ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct tacttcctgg
    15781 tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac cgcaaactga
    15841 ttggtcgcca tttgccttgc gatatctgtg ttgtggaaga aaaggagacc acaactcctt
    15901 cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac tgaaaaaaaa
    15961 tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat ggatcacgat
    16021 gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa accaggctgg
    16081 gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt agaagtgctg
    16141 gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa ccagcgtgaa
    16201 accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat tgtctggcag
    16261 tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga agattatatc
    16321 cgcagcaata ccggtctggt gattgacccg tatttctctg gcaccaaagt gaagtggatc
    16381 ctcgaccatg tggaaggctc tcgcgaacgt gcgcgtcgtg gtgaattgct gtttggtacg
    16441 gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac cgattacacc
    16501 aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga caaaatgctg
    16561 gaggtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc cgaagtatac
    16621 ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg gatcgccggt
    16681 gaccagcagg cggcgctgtt tggtcagttg tgcgtgaaag aagggatggc gaaaaatacc
    16741 tacggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa atcagaaaac
    16801 ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc gctggaaggt
    16861 gcggtgttta tggcgggcgc gtccattcag tggctgcgcg atgagatgaa gttgattaac
    16921 gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg tgtgtatgtg
    16981 gttccggcct ttaccgggct gggtgcgcca tactgggatc cgtatgcacg cggggcgatt
    17041 ttcggtctga ctcgtggggt gaacgctaac cacattatac gcgcgacgct ggagtctatt
    17101 gcttatcaga cgcgtgacgt gctggaagca atgcaggccg actccggtat tcgtctgcac
    17161 gcgctgcgcg tggacggcgg tgcggtggcg aacaatttcc tgatgcagtt ccagtccgat
    17221 attctcggta cgcgcgttga gcgcccggaa gtgcgcgaag tcaccgcttt gggtgcggcc
    17281 tatcttgctg gtctggcggt tggcttctgg cagaatcttg acgagctgca agagaaagcg
    17341 gtgattgaac gcgagttccg tcctggcatc gaaaccactg agcgtaatta ccgttacgca
    17401 ggctggaaaa aagcggtcaa acgcgcgatg gcatgggaag aacacgacga gtaataatga
    17461 ttgcctgatg cgacgcttgc gcgtcttatc aggcctacga gtcggataca catccggcat
    17521 cacacagact ctgaaaccgt gcgggggcaa ccccgcacac atcaataatc ccgcccttcc
    17581 cctgtgctac acttcgcgcc attccttact gcttagagtt tgctatgaga cgagaacttg
    17641 ccatcgaatt ttcccgcgtc accgaatctg cggcgctggc tggctacaaa tggttaggac
    17701 gcggcgataa aaacaccgcg gacggcgcgg cggtaaacgc catgcgtatt atgctcaacc
    17761 aggtcaacat tgacggcacc atcgtcattg gtgaaggtga aatcgacgaa gcaccgatgc
    17821 tctacattgg tgaaaaagtc ggtactggtc gcggcgacgc ggtagatatt gctgttgatc
    17881 cgattgaagg cacgcgcatg acggcgatgg gccaggctaa cgcgctggcg gtgctggcag
    17941 taggcgataa aggctgtttc ctcaacgcgc cagatatgta tatggagaag ctgatcgtcg
    18001 gaccgggagc caaaggcacc attgatctga acctgccgct ggcggataac ctgcgcaatg
    18061 tagcggcggc gctcggcaaa ccgttgagcg aactgacggt aacgattctg gctaaaccac
    18121 gccacgatgc cgttatcgct gaaatgcagc aactcggcgt acgcgtattt gctattccgg
    18181 atggcgacgt tgcggcctca attctcacct gtatgccaga cagcgaagtt gacgtgctgt
    18241 acggtattgg tggcgcgccg gaaggcgtgg tttctgcggc ggtgatccgc gcattagatg
    18301 gtgacatgaa cggtcgtctg ctggcgcgtc atgacgtcaa aggcgacaac gaagagaatc
    18361 gtcgcattgg cgagcaggag ctggcacgct gcaaagcaat gggcatcgaa gccggtaaag
    18421 tattacgtct gggcgatatg gcacgcagcg ataacgtcat cttttctgcc accggtatta
    18481 ccaaaggcga tctgctggaa ggcattagcc gcaaaggcaa tatcgcgact accgaaacgc
    18541 tgctgatccg cggcaagtca cgcactattc gccgcattca gtccatccac tatctggatc
    18601 gcaaagaccc ggaaatgcag gtgcacattc tctgagtgat ttgatcgatt gagccttcca
    18661 gtccttcggg actggaattt ttttgttcgg agaacgaaga taaggcaagt caatcaaaac
    18721 aggagaaaaa catggctgat tgggtaacag gcaaagtcac taaagtgcag aactggaccg
    18781 acgccctgtt tagtctcacc gttcacgccc ccgtgcttcc gtttaccgcc gggcaattca
    18841 ccaagcttgg ccttgaaatt gacggcgaac gcgtccagcg cgcctactcc tatgttaact
    18901 cgcccgataa tcccgatctg gagttttacc tggtcaccgt ccccgatggc aaattaagcc
    18961 cacgattggc ggcactgaaa ccgggcgatg aagtgcaggt ggttagcgaa gcagctggct
    19021 tctttgttct ggatgaagta ccagattgcg aaacgctatg gatgctggca accggtacag
    19081 cgattggccc ttatttatcg attctgcaac tgggcaaaga tttagatcgc ttcaaaaatc
    19141 tggtcctggt tcacgccgca cgttatgccg ccgacttaag ctatttgcca ctgatgcaag
    19201 aactggaaaa acgctacgaa ggcaaactgc gcattcagac ggtggtcagt cgggaaacgg
    19261 cagcggggtc gctcaccgga cggataccag cgttaattga aagtggtgaa ctggaaagcg
    19321 cggttggcct gccgatgaat aaagaaacca gccatgtgat gctgtgcggc aatccacaga
    19381 tggtgcgcga tacgcaacag ttgctgaaag agacccggca gatgacgaaa catttacgtc
    19441 gccgaccggg ccatatgaca gcggagcatt actggtaagc ggttacttat cgataaacgg
    19501 cacgatgagc aaatccgcac tcaacttatt gatcatcccg cgatatgccg gcatcaaacg
    19561 gttgataaat gagtggtgat gaccacagac aaggaggtcg cactgctctt tttgcataat
    19621 ttccagcagt gtttccggca tttctccgcg ttcaatacgc agttttgtct tcggccattg
    19681 aatatttttc gtcagtttat acagcttgtt atccgactta ttcttcaaca attgaagaat
    19741 atcttctgtt gcagggaagt agatacccgg atacaactcg cttaagccat catcaatatg
    19801 aattaacgtc aggtgagcgt cattatgtct ggcgagctct agggctttat tcaccagtaa
    19861 ggcatcttct tcattcccgg aaattgccac gccaatgtgt ttataagcca tatttaactc
    19921 cttctaaagc aactccatca agctaacgaa cgcagtgata actcaaaaat aatcatctcg
    19981 gcctggcatg agaaggtgaa ggccgcatca agctcaactt gcccttccag atccttaatc
    20041 ttataggcca cgctggcccc ttcatctgca gaggatgcag cggcggcaag ctctttcagt
    20101 ttggtcataa aggactctgc ttcttcgcgg gtggcaaaaa agcgcgaaaa cttactggta
    20161 cagttgtcgt tatcaatcac cgtaccgata tcaatcgcac atcctttagt actgcattta
    20221 tctacgacat ctttcatagg gcacctctgt atattcgcct ctctgtcgat tacgacttaa
    20281 gaataatgcg ccgttaaagg aagaacaaac aagtaatgat cttatggatt accatttttt
    20341 ttgatattgc ttaaggtgaa gagttcagaa ggattttccg ggcattgtca cttgcatcgt
    20401 ggaaataaca atcccggaaa aaaatagcga taagagatat attaaaggtt aattacttga
    20461 tttattgtcg gctttatact tcacatcctg ggtatcttta ccatatttat tttcgccttg
    20521 tgtgccaaca aacgcaccaa gatcgataag catcatcacc agaatcaatg tcgggataaa
    20581 acgccccacc gcccattgcc agacacccgg taaaattgcc cagttacccg ccagcagcat
    20641 ccacgccaca atcatcagaa atgcccatgc gccggaacgc ccgcgatcat gcaagcgctt
    20701 aacggttact gccgctgttg gccaaagcaa gcacacaagg caaaacgccg cggtctgaat
    20761 atcgagtaaa tttttacccg ccagtgagaa aagcaccagc atgcctgcga accacaggcc
    20821 tatccaaatc cagaaatcac ggcgtccaat acgcccttta aatgagaata accattgctg
    20881 tatggtcatg taagttcctt gatggttgtc ttttccagga ttctaccctt ttgacaaggg
    20941 cgacagatat cgttttaatc ggagccagtc ataacaaaag gtactgtcaa tgaagccagg
    21001 gtgtacgctg ttttttctct tatgttctgc attaaccgtt acaacaacgg cgcatgcaca
    21061 aacaccagat acggcaacga ccgcgcctta tctgctggct ggagccccta ctttcgatct
    21121 ctccatcagc cagtttcgag aagactttaa cagccagaat cccagcctgc cactgaacga
    21181 atttcgtgcc atcgacagca gtcccgacaa agccaatctc actcgtgcag caagtaaaat
    21241 taacgagaac ttgtatgctt ctactgcgct ggagcgcggt accttaaaaa tcaaaagcat
    21301 tcaaataacc tggctaccca tccaggggcc agagcaaaaa gccgcgaaag cgaaagctca
    21361 ggcatacatg gcagcggtga tccgcacact caccccatta atgaccaaaa ctcaaagcca
    21421 gaaaaaactg cagtcgctac taacggcggg gaaaaacaaa cgttattaca ccgagacaga
    21481 aggtgcactg cgttatgttg tcgcggacaa cggcgaaaag gggctgacct tcgctgttga
    21541 accgattaag ctggcgctat ctgaatcgct tgaaggtttg aataaatgac aaaaagcaaa
    21601 gcctttgtgc cgatgaatct ctatactgtt tcacagacct gctgccctgc ggggcggcca
    21661 tcttccttta ttcgcttata agcgtggaga attaaaatgc gacatccttt agtgatgggt
    21721 aactggaaac tgaacggcag ccgccacatg gttcacgagc tggtatctaa cctgcgtaaa
    21781 gagctggcag gtgttgctgg ctgtgcggtt gcaatcgcac caccggaaat gtacatcgat
    21841 atggcgaagc gtgaagctga aggcagccac atcatgctgg gtgcgcaaaa cgtggacctg
    21901 aacctgtccg gcgcattcac cggtgaaacc tctgctgcta tgctgaaaga catcggcgca
    21961 cagtacatca tcatcggcca ctctgaacgt cgtacttacc acaaagaatc tgacgaactg
    22021 atcgcgaaaa aattcgcggt gctgaaagag cagggactga ctccggttct gtgcatcggt
    22081 gaaaccgaag cggaaaacga agcgggcaaa actgaagaag tttgtgcacg tcagatcgac
    22141 gcggtactga aaactcaggg tgctgcggca ttcgaaggtg cggttatcgc ttacgaacct
    22201 gtatgggcaa tcggtactgg caaatctgca actccggctc aggcacaggc tgttcacaaa
    22261 ttcatccgtg accacattgc taaagttgac gctaacatcg ctgaacaagt gatcattcag
    22321 tacggcggct ctgtaaacgc gtctaacgct gcagaactgt ttgctcagcc agacatcgac
    22381 ggcgcgctgg ttggcggtgc ttctctgaaa gctgatgctt tcgccgtgat cgttaaagct
    22441 gcagaagcgg ctaaacaggc ttaagtctga caggtgccgg atttcatatc cggcacttac
    22501 tttcctaaac tcctcgcctt aacgcaaaat ctcacactga tgatcctgga tttcttcggc
    22561 tgaagcacgg ttcagcgtca gtagatttcg ttgtgtcgcc agcaatacaa atgagttatc
    22621 actctgccgt accatcgcca gcccgtagct tcccatatgt tcccgcgcct caggcacttc
    22681 ttccgccagc atcataaatg ggctgcgttg taccagttcg ctttccgtta cccgacgcgc
    22741 caggtactca tgcccacgca aaccacctgg cagtggcaac cagcggctac tgatgttcgc
    22801 cagattgtta tccagctgtt cgcgcacatc aggacgaata caagaaatat gaatatgaaa
    22861 atggttttgc gtacgcccgg tgcgggagtt gatcgccaga gataccgcgc gatcgggaac
    22921 aggctggccg tatttttggc tcataaaatc acgcgcctgc caggctaacc agaaaaaatt
    22981 aggcgttgac ggatcggtca acaatgggct ttcagtaccg ttaatacggt acgttggcat
    23041 caacagatat tgcagtgggc catgacgatc ttttaaaacc acgtatccgg cgttgggttt
    23101 gacttccgca catggcgacg ggttttggtt ttcctgctgg ttgggcaaac attgctcaag
    23161 aacaatctta cgtaacgtgt ctgactcttc accggttaat ttccagtaac caataccggc
    23221 agccacaacg gcgataacta tcatcaccaa aaaaagaaga cccgcttttt tcatcttttt
    23281 ttccctgtac ctcaaagaga tgcaagggta acgcaaaatc gtgacaaata aaaaacccag
    23341 tcgcgagaaa ccaccgggct tgataattat cctggggaaa tcagcgtttg ctgatctgat
    23401 cgaacgtacc gccgttagca aaatgctctt tttgcgcttt cgtccagccg ccgaactctt
    23461 catcaatggt gaataacttc aattttggaa acgcattttc gtactttttc gctacctcag
    23521 cgtcgcgcgg acggtagtag tttttcgctg caatttcctg accttctggc gagtagagat
    23581 atttcaggta ggcttccgcc acctctttgg tgcctttttt ctcgaccact ttatcgacca
    23641 ccgacacggt tggctctgcg aggatagact cactcggcgt gacgatttcg aatttatctt
    23701 tccccagttc attcgctgcc agcagagctt cattttccca ggcaatcagt acatcgccaa
    23761 ttccgcgctc gacaaaagtg ttagtggatc cgcgcgcgcc agaatccaga acttcgacgt
    23821 ttttatacag tgcccgaaca aaatcctgtg cttttgcctg atcgttgttg ttgtgatgca
    23881 gcgcgtagcc ccaggctgcc aggtaattcc agcgcgcgcc accagagctt ttcggattag
    23941 gcgtgatcac cgaaacaccc ggtttaatca gatcgttcca gtcatggatc tgcttcggat
    24001 tacccttacg taccaggaaa acaatggtgg aagtgtacgg tgcggagtta tccggcagac
    24061 gtttgatcca ctctttatca atccgcccgc gttcagcaat cgcgtccacg tcataggcca
    24121 gagccagcgt gacaacatca gcttcaatac cgttgattac cgacgtcgct tgtttgcctg
    24181 aaccaccgtg tgactgacga atcaccacgt tatcgccagt ttgctgtttc cagtgggcgc
    24241 tgaatgcctt gttgtactgt tcgtacaatt cgcgcgttgg atcatatgaa acgttaagaa
    24301 gctgaatatc ctttgccata acgctggttg ccgccagcaa aaatgttaac cctacgcccc
    24361 acttgttcat cgcccgactc tcttatgttg tgttgtgatg agcaaagcgt gccagaaggt
    24421 taaccaaaca ttaaagaata aaaaaagatt ggctataact tgcgggtata tgttggggga
    24481 ttaaaaaggc ggaagaaatc cgcctcatat tgctgacaaa gtgcgctttg tccatgccgg
    24541 atgcggcgcg aacgccttat ccggcctaca aaagtttgca aattcaatag attacagaat
    24601 tcatgtaggc ctgataagcg aagcgcatca ggcatttttg cttctgtcat cggtttcagg
    24661 ctaaaggaat ctgccttttt ccgaaatcat taatacagtt ttttcgcgca gtccagccag
    24721 tcgcctttga acggacgctt catattttcg atagcgtcga tgatgtcgtg gtgaaccagc
    24781 tgttcgttct ggatacctac gcaacgaccg ccgtaacctg ccagcagcag atcgatagcg
    24841 taagcgccca tacgggaagc cagaatacgg tcgtaaggca ccggagaacc accgcgctgg
    24901 atgtggccca gcacagttgc gcgggtttca cgaccggttt ctttctcgat gaaatgcgcc
    24961 agttcgtcaa catcacacat atgttcggta atcgccacga tcgcgtgttt tttacctttc
    25021 gcgatacccg ctttgatttc gtttaccaga tcttcacggc tgaattcaac ttccggaacc
    25081 acaacgaatt cacagccacc ggcaatggcc gcagccaacg tcagatcgcc acaataacgg
    25141 cccatcactt ccaccacgga aatacgctgg tgagaagaag aggtgtcacg cagacggtcg
    25201 atcgcttcta caacggtgct cagcgcagtg aagaaaccga tagtgtagtc agtgcctttg
    25261 atgtcgttgt cgatagtgcc cggcagaccg atgcacggga agcccatttc ggtcagacgc
    25321 attgcaccca tgtaggaacc gtcaccgccg ataaccacca gcgcgtcgat accacgtttt
    25381 ttcaggtttt cgatagccac ggcgcggatg ttctcgtcgc ggaattccgg gaaacgcgca
    25441 gaaccgagga acgtaccacc acggttgatc atgtcagaca cgctgtaacg gtctagctgt
    25501 accatacggt cttcatacag acccagatag ccgtcataaa tgcccattac ttccagacct
    25561 tctgtcagcg cagaacgaac aaccccgcga attgcggcgt tcatgcctgg cgcatcaccg
    25621 ccgcttgtca acacaccgat tttcttaatc atgactacct ctgaactttg gaatgcaaaa
    25681 tgaaatctgt tgccggaagt cttcttgcac atcgaagtga tccaacgaat gtgcaaatag
    25741 tataacaatc acttcctgct gaattgattc aggtcaggcc aaatggcggt attttataca
    25801 caaaatgcgg gtctggctct cttttatact gattatgaaa gcatagaccg tttaccctcc
    25861 ctgggtacga cggaacaggg gtcctgatgg ataatcacat ccgatcccgg aaaacgccgt
    25921 aaaatagcct gctctacctg atccgccacc atatgtgcct gaaccaaagg cagagagtct
    25981 tccatttcca aatgaatctg aataaagcgg gtcggccctg actgccgcgt gcgaagatcg
    26041 tgagcgccgc taacacccgg ccaggaagtc acgatatcaa taatttcttg ccgttcctca
    26101 tcaggcaatg cgcgatccag taatgactgt accgcctcat atcccatgcg taacgcgcta
    26161 tataaaatat agatgccgat ccccaatgca aacagagcat cggcgcgatg ccagccgtac
    26221 caggacaacc ccagcgccag cagaattgcg ccgttcatca taacatcaga ctggtaatgt
    26281 agcatatcag cccgcaccgc ctggctttgc gtccggcgca ccacccaacg ctgaaacgag
    26341 acaaggataa tcgtacaaat tagcgccaca attgtcacga taaccccgac gcctggatct
    26401 gtcatcggtg ttggagatac cagatgttga atacccgtca aaaacaggaa tagtgccgaa
    26461 ccggagataa acatactttg cgccagcgcc gcgagggact cagctttacc gtgaccaaac
    26521 gagtgattat cgtcggcagg ttgcagggaa tatcgcacca ccagtaaatt cgtcaacgac
    26581 gcgccgatat ccaccagcga atccaccagc gcggcgagaa tactcaccga cccggtatac
    26641 caccatgcaa aaattttaat cagcaatagc agcgaagcca tcgccgtcgc agcaatcgcc
    26701 gcccgactga ccagccgtcc ataagattga ttcataaata ctcccgctat caactgacgg
    26761 tagtataacg gaagcaaatc atctgcaatg cattaagcag caggcaaatt gaggataaaa
    26821 aaaaccccca catcatgtgg gggaagacag ggatggtgtc tatggcaagg aaaacagggt
    26881 ttactactgg gaacgtgagt tgctactact caatagcttc aacgatgaac ttttttgcca
    26941 ttgcgtcacg tcgcgcaact gctccattcg ttgttgatgt ttctcgttta aaaccgcttg
    27001 ctgctccggc gttaacaggc gatacatttg gttgcggact tttgctatct caacctgacg
    27061 agctatttgc tcgttcgcca ttttttctgc ctgtgcgcgc acagcgtttt catcaaaatt
    27121 ttctgcggtg acaaggcgat gcattgtctc cagttcgcta acattaacag gaggctgttc
    27181 gtgccgggcc tgttgcataa gatctcgcat ctgctgacgc tgatgttcgg ttaaacttat
    27241 gccgtcgaac atatggctct gcgtactgcg ctgcgtaagt tcttcacccg gatgccagtt
    27301 atcgcctgaa ccgacttcag cagcgtggct taatgaactg actgccagcg ttgaggccat
    27361 gacggcagcg gtaactatgc gcatcatttg ctcccaaaat ctttctgtcg cgattcaacg
    27421 atagagagtt tacgattcag gctgcaaaca tgcgtcaggg ggtgtaaaac aacgtaaagt
    27481 catggattag cgacgtctga tgacgtaatt tctgcctcgg aggtatttaa acaatgaata
    27541 aaatcctgtt agttgatgat gaccgagagc tgacttccct attaaaggag ctgctcgaga
    27601 tggaaggctt caacgtgatt gttgcccacg atggggaaca ggcgcttgat cttctggacg
    27661 acagcattga tttacttttg cttgatgtaa tgatgccgaa gaaaaatggt atcgacacat
    27721 taaaagcact tcgccagaca caccagacgc ctgtcattat gttgacggcg cgcggcagtg
    27781 aacttgatcg cgttctcggg cttgagctgg gcgcagatga ctatctccca aaaccgttta
    27841 atgatcgtga gctggtggca cgtattcgcg cgatcctgcg ccgttcgcac tggagcgaac
    27901 aacagcaaaa caacgacaac ggttcaccga cactggaagt tgatgcctta agtctcaatc
    27961 ctggtcgcca ggaagccagc ttcgacgggc aaacgctgga gttaaccggt actgagttta
    28021 ccctgctcta tttgctggca cagcatctgg gtcaggtggt ttcccgtgag catttaagcc
    28081 aggaagtgct gggcaagcgc ctgacgcctt tcgaccgcgc tatcgatatg cacatttcca
    28141 acctgcgtcg taaactgccg gatcgtaaag atggtcaccc gtggtttaaa accctgcgtg
    28201 gtcgcggcta tctgatggtt tctgcttcat gataggcagc ttaaccgcgc gcatcttcgc
    28261 catcttctgg ctgacgctgg cgctggtgtt gatgttggtt ttgatgttac ccaagctcga
    28321 ttcacgccag atgaccgagc ttctggatag cgaacagcgt cagggtctga tgattgagca
    28381 gcatgtcgaa gcggagctgg cgaacgatcc gcccaacgat ttaatgtggt ggcggcgtct
    28441 gttccgggcg attgataagt gggcaccgcc aggacagcgt ttgttattgg tgaccaccga
    28501 aggccgcgtg atcggcgctg aacgcagcga aatgcagatc attcgtaact ttattggtca
    28561 ggccgataac gccgatcatc cgcagaagaa aaagtatggc cgcgtggaac tggtcggtcc
    28621 gttctccgtg cgtgatggcg aagataatta ccaactttat ctgattcgtc cggccagcag
    28681 ttctcaatcc gatttcatca acttactgtt tgaccgcccg ctattactgc tgattgtcac
    28741 catgctggtc agtacgccgc tgctgttgtg gttggcctgg agtctggcaa aaccggcgcg
    28801 taagctgaaa aacgctgccg atgaagttgc ccagggaaac ttacgccagc acccggaact
    28861 ggaagcgggg ccacaggaat tccttgccgc cggtgccagt tttaaccaga tggtcaccgc
    28921 gctggagcgc atgatgacct ctcagcagcg tctactttct gatatctctc acgaactgcg
    28981 caccccgctg acgcgtctgc aactgggtac ggcgttactg cgccgtcgta gcggtgaaag
    29041 caaggaactg gagcgtattg aaactgaagc gcaacgtctg gacagcatga tcaacgatct
    29101 gttggtgatg tcacgtaatc agcagaaaaa tgcgctggtt agcgagacca tcaaagccaa
    29161 ccagttgtgg agtgaagtgc tggataacgc ggcgttcgaa gccgagcaaa tgggcaagtc
    29221 gttgacggtt aacttcccac ctgggccgtg gccgctgtac ggcaacccga acgccctgga
    29281 aagtgcgctg gaaaacattg ttcgtaatgc tctgcgttat tcccatacga agattgaagt
    29341 gggctttgcg gtagataaag acggtatcac cattacggtg gacgacgatg gtcctggcgt
    29401 tagcccggaa gatcgcgaac agattttccg tccgttctat cggaccgacg aagcacgcga
    29461 tcgtgaatct ggcggtacag gtttggggct ggcgattgtt gaaaccgcca ttcagcagca
    29521 tcgtggctgg gtgaaggcag aagacagccc gctgggcggt ttacggctgg tgatttggtt
    29581 gccgctgtat aagcggtcat aacgtcaaca ttcaggtaaa aaaatgcctg atgcactacg
    29641 cttatcaggc ctacaaaacc tgttgaattt atgggttttg taggcaggat aaggcgttta
    29701 cgccgcatcc ggcatttgag caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc
    29761 tacactccgg ggttattttt cccacaatcg gcgggaaaaa tcttcgatct tgccgctaag
    29821 tcggcgcttt tgcatcgttc gcgtccagct tttcgataac cctgcggcgg agagtaaacg
    29881 gtgatactga tcgtcatcaa acggcatatg ccatgcgatg gcggcagcct cctgcacggt
    29941 cacatcgctg acacgggaaa ccaactccag tggcgcatca gcagatacca accctggtgc
    30001 aattacgcta tacaaccacc ccactttacc cgtgttttgc atcaactgcg ctatatcgct
    30061 gatatcaaaa tggtaattga gtttatagca aggtgagcgt ggctggctga cctgaattaa
    30121 tgcctctccc cagcggaaaa tatcaccaat ataaacatta ctttccgtca gaccgtcggt
    30181 tgagagattt tcgccaaacg caggcgcaac aaacagctcc gcctgttccg gaaattcccg
    30241 cgcccagtag agataatgct cacgaggata atgacacagc gctctgtctg gcccgccgtg
    30301 gatctttttc tccgcctgtt cgtcaccttc cagccccagg tccgtcagca tcaactcacc
    30361 atcaacctgg attttggcaa ttgcgctggg tttgccttcg ggataagcct gaatcttgcc
    30421 tgtgtataca tcaaccggat atcgcatctg ttccctctcc acgagaaatt ttcccattag
    30481 accatattta ttttatgtca ggcgtgctgt tttcgcggta agtatccagg cagaaacgca
    30541 attgaaaacc ggacgaacgt tagttcatcc ggctgtgggg attaaggaag caggtttatt
    30601 ttaccgtgcc taaaatttcg attttcgcca ctctggcttt gactttacgt gaccagatag
    30661 aggtaagaat tggcaccaga atcgaagtca caatgaccgc cgtcgcgacc agcgaagttg
    30721 ctgccggagc catcggttta aacgcaggca ccatttcagc aatcagcaca ggtgtcgcta
    30781 ccgcggcccc tgcggaactg gaagcagcaa ttccggcagt gccatcgcca ccgccaatca
    30841 atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga
    30901 tccccagcaa acctgtctgg gcaattacag tcaaatcaat ggtattgccc agtgcgaagg
    30961 caaagaatgg aatcagcgtc tgcaccgctt tgctgaaaaa ttctcgtaac tcagggtcaa
    31021 ggttccccag ggcaaagccc accaggaacg gcaatactgc gccgacgaaa acatgcggtt
    31081 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
    31141 acgacatcaa cacaaatgcc ccagcttctt cttttgtgcc gtactgctgc atgatggaag
    31201 cgtaaagtcc gccgttggtc atatccatcg ccgccaccag cgccaacgtt gaaagtccgg
    31261 caaaaaatcc aacttcaaca ccatgttccg gaataatgcg cgaggcaatt gccgcaacca
    31321 cccacgcgac ggcaatttta gttaccacca gcgtaccgga tttacgcagt accgttcccg
    31381 tcgcgcttaa ttttattgac gccccatgca aaaaaaccac accgccagaa tgggcaccgt
    31441 accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt
    31501 gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat
    31561 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtcat
    31621 tcctcaacgg agcatcagtg ttgatggaaa cgaagataga cattcatttg atcaataaaa
    31681 gtgatcaaaa ccacaccaat tgaaacgctg ttttattttt atcggatcat tgtttttatt
    31741 ttgaaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgcaat
    31801 gcggcgtaaa cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcgaaacgtg
    31861 ccgctgcttc gtcccagttc accacgttcc agaactcttt aatgtagtcc ggacggcggt
    31921 tctggaattt cagatagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
    31981 aagcgccaga aatggcttcg cccatcagtg gagagtcctg gttagcagta gaaaccaccg
    32041 ccagtttatc gcctttttgc accagccatg cccagccgga accaaagcgg gaagctgccg
    32101 ctttttcaaa ttctgctttg aaattatcaa cggaaccgaa atcacgttcg atagccgctt
    32161 tcaggtcgcc ctgcagggtg gtgccttttt tcagaccttt ccagaacaga ctgtggttag
    32221 cgtgaccacc agcgttattg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
    32281 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggttttcc agcgccgcgt
    32341 tggcgttgtt gacgtaagtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
    32401 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta taactcatat
    32461 tcatctccag tgttgtcggg cggccgattg ttaatgccgc gtaagcagtt ggttcattat
    32521 agttaattaa atgatattga aaatgattat caatgccgta cttttcataa gggtatggtt
    32581 ttgcaggaaa atgcccaaga tgtgaagcaa atcacccact taatgccgtg attgccagta
    32641 aatcggcagc ggcggcaaca ggcgaaaggt taatcgacag ctcaattttt acactcatct
    32701 cgtcggagat gtgacgcgac gaaaaaatga tgaggataag aagatgagta acgcgattac
    32761 gatggggata ttttggcatt tgatcggcgc ggccagtgca gcctgttttt acgctccgtt
    32821 caaaaaagta aaaaaatggt catgggaaac catgtggtca gtcggtggga ttgtttcgtg
    32881 gattatcctg ccgtgggcca tcagcgccct gttactaccg aatttctggg cgtattacag
    32941 ctcgtttagt ctctctacgc tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg
    33001 taatatcaac tacggcctga ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc
    33061 cattggcatt acgttgattg tcggtacgct gatgacgcca attatcaacg gcaatttcga
    33121 tgtgctgatt aataccgaag gcggacgcat gacgttgctc ggcgttctgg tggcgctgat
    33181 tggcgtaggg attgtaactc gcgccgggca gttgaaagag cgcaagatgg gcattaaagc
    33241 cgaagagttc aatctgaaaa aagggctggt gctggcggtg atgtgcggca ttttctctgc
    33301 cgggatgtcc tttgcgatga acgccgcaaa accgatgcat gaagccgctg ccgcgctggg
    33361 cgtcgatccc ctgtatgtcg ctctgccaag ctatgttgtc atcatgggca gcggcgcaat
    33421 catcaacctc ggcttctgct tcattcgtct ggcaaaagtg aaagatttgt cgctaaaagc
    33481 cgacttctcg ctggcaaaac cgctgatcat tcacaatgtg ttactctcag cactgggcgg
    33541 gttgatgtgg tatctgcaat tctttttcta tgcctggggc cacgcccgca ttccggcgca
    33601 gtatgactac atcagctgga tgctgcatat gagcttctat gtattgtgcg gcggtatcgt
    33661 cgggctggtg ctgaaagagt ggaacaatgc aggacgccgt ccggtaacgg tgttgagcct
    33721 cggttgtgtg gtgattattg tcgccgctaa catcgtcggc atcggcatgg cgaattaatc
    33781 tttctgcgaa ttgagatgac gccactggct gggcgtcatc ccggtttccc gggtaaacac
    33841 caccgaaaaa tagttactat cttcaaagcc acattcggtc gaaatatcac tgattaacag
    33901 gcggctatgc tggaggagat attgcgcatg acacactctg acctgtcgca gatattgatt
    33961 gatggtcatt ccagtctgct gacgaaattg ctgacgcaaa acgcgctcac tgcacgatgt
    34021 ctcatcacaa aatttatcca gcgcaaaggg acttttcagg ctagccgcca gccgggtaat
    34081 cagcttatcc agcaacgttt cgctggatgt tggcggcaac gaatcactgg tgtaacgatg
    34141 gcgattcagc aacatcacca actgcccgaa cagcaactca gccatttcgt tagcaaacga
    34201 cacatgctga ctactttcat gctcaagctg accgataacc tgccgcgcct gcgccatccc
    34261 cacgctacct aagcgccagt gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc
    34321 ctgccagtca agattcagct tcagccgctc cgggcaataa ataatattct gcaaaaccag
    34381 atcgttaacg gaagcgtagg agtgtttatc gtcagcatga atataaaaga gatcgccacg
    34441 ggtaatgcga taagggcgat cgttgagtac atgcagacca ttaccacgcc agacaatcac
    34501 cagctcacaa aaatcatgtg tatgttcggc aaagacatct tgcggataac ggtcagccac
    34561 agcgactgcc tgctggtcgc tggcaaaaaa atcatctttg agaagtttta actgatgcgc
    34621 caccgtggct acctcggcca gagaacgaag ttgattattc gcaatatggc gtacaaatac
    34681 gttgagaaga ttcgcgttat tgcagaaaac catcccgtcc ctgacgaata tcacgcggtg
    34741 accagttaaa ctctcggcga aaaagcgtcg aaaagtggtt actgtcgctg aatccgcagc
    34801 gataggcgat gtcagtaacg ctggcctcgc tgtggcgtag cagatgtcgg gctttcatca
    34861 gtcgcaggcg gttcaggtaa cgctgaggcg tcagccccgt ttgctgctta agctggcgat
    34921 gcagcgtacg caatgaaaga gaaaattgat ccgccacagc gtcccaattc acctcatcgg
    34981 caaaatggtc ctccagccag gccagaagca agttgagacg tgatgcgctg ttttccaggt
    35041 tctcctgcaa actgctttta cgcagcaaga gcaataattg cataaataag atctcgcgac
    35101 tggcggtcga gggtaaatca ttttcccctt cctgctgttc catctgtgca accagctgtc
    35161 gcacctgctg caatacgctg tggttaacgc gccagtgaga cggatactgc ccatcctgct
    35221 cttgtggcag caactgattc agcccggcga gaaactgaaa tcgatccggc gagcgataca
    35281 gcacattggt cagacacaga ttatcggtat gttcatacag atgccgatca tgatcgcgta
    35341 cgaaacagac cgtcccaccg gtgatggtat agggctgccc attaaataca tgaatacccg
    35401 tgccatgttc gacaatcaca atttcatgaa aatcatgatg atgttcagga aaatccgcct
    35461 gcgggagccg gggttctatc gccacggacg cgttaccaga cggaaaaaaa tccacactat
    35521 gtaatacggt catactggcc tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt
    35581 caggttctta ccttaaattt tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat
    35641 acagcgtgaa ttttcaggaa atgcggtgag catcacatca ccacaatgaa gcaaattgtg
    35701 aacatcatca cgttcatctt tccctggttg ccaatggccc attttcctgt cagtaacgag
    35761 aaggtcacga attcaggcgc tttttagact ggtcgtaatg aaattcagca ggatcacatt
    35821 atgacctttc gcaattgtgt cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg
    35881 gcgcgttacg agcgtgaatg ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat
    35941 ggcctgcata gccagaacgg ttatgtcacc tggaatgtgg atagcctgga aagtgccatt
    36001 cgccttggat taaacaaggt gtgcgaggaa gggattcgta tcgatagcat tgggattgat
    36061 acctggggcg tggactttgt gctgctcgac cagcacggtc agcgcgtggg tctacccgtt
    36121 gcttaccgcg atagccgcac ccatggccta atggcgcagg cacaacagca actaggcaaa
    36181 cgcgatattt atcaacgtag cggcatccag tttctgccct tcaatacgat ctatcagcta
    36241 cgtgcactga cagagcagca accagaactt attccacaca ttgctcacgc tttgctgata
    36301 ccggattact ttagttatcg cctgaccggc aagatgaact gggaatatac caacgccacg
    36361 accacgcaac tggtcaatat caatagcgac gactgggacg agtcgctact ggcgtggagc
    36421 ggtgccaaca aagcctggtt tggtcgcccg acgcatccgg gtaatgtcat aggtcactgg
    36481 atttgcccgc agggtaatga gattccggtg gtcgccgttg ccagccatga taccgccagc
    36541 gcggttatcg cctcgccgtt aaacggttca cgcgccgctt atctctcttc tggcacctgg
    36601 tcattgatgg gcttcgaaag ccagacgcca tttaccaatg acacggcgct ggcagccaat
    36661 atcaccaacg aaggcggggc ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta
    36721 tggctgcttc agcgagtgct tcaggagcgg caaatcaacg atcttccggc gcttatcgcc
    36781 gcaacacagg cacttccggc ctgccgcttc atcatcaatc ccaatgacga tcgctttatt
    36841 aatcctgacg agatgtgcag cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc
    36901 ccggaaagtg atgctgaact ggcgcgctgt attttcgaca gtctggcgct gctgtatgcc
    36961 gatgtgttgc atgagctggc gcagctgcgt ggtgaagatt tctcgcaact gcatattgtc
    37021 ggcggcggct gccagaacac gctgctcaac cagctgtgtg ccgatgcctg cggtattcgg
    37081 gtgatcgcag ggcctgttga agcctcgacg ctcggcaata tcggcatcca gttaatgacg
    37141 ctggatgaac tcaacaatgt ggatgatttc cgccaggtcg tcagcactac cgcgaatctg
    37201 accaccttta cccctaatcc tgacagtgaa attgcccact atgtggcgca gattcactct
    37261 acacgacaga caaaggagct ttgcgcatga ccactcaact ggaacaggcc tgggagctgg
    37321 cgaaacagcg tttcgcggca gtagggattg atgtcgagga ggcactgcgc caacttgatc
    37381 gtttacccgt ttcaatgcac tgctggcagg gcgatgatgt ttccggtttt gaaaacccgg
    37441 aaggtttgct gaccggtggg attcaggcta ctggtaatta tccgggcaaa gcgcgtaatg
    37501 ccagtgagct acgtgccgat ctggaacagg ctatgcggct gattccaggg ccgaaacggc
    37561 ttaatttaca tgccatctat ctggaatcag acacgccagt ctcgcgcgac cagatcacac
    37621 cagagcactt caaaaactgg gttgaatggg cgaaagccaa tcagctcggt ctggatttta
    37681 acccctcctg tttttcgcat ccgctaagcg ccgatggctt tacgctttcc catgccgacg
    37741 accgcattcg ccagttctgg attgaccact gtaaagccag ccgccgtgtt tcagcctatt
    37801 ttggtgagca actcggcaca ccatcggtga tgaacatctg gatcccggat ggcatgaaag
    37861 atattaccgt tgaccgtctc gccccacgcc agcgtttgct ggcagcactg gatgaggtga
    37921 tcagcgagaa gctaaaccct gcgcaccata tcgacgccgt tgagagcaaa ttgtttggca
    37981 ttggcgcgga gagctacacg gttggctcca atgagtttta cctggggtat gccaccagcc
    38041 gccagacagc gctgtgcctg gacgccgggc atttccaccc gactgaagtg atttccgaca
    38101 agatttccgc cgccatgctg tatgtgccgc agttgctgct gcacgtcagc cgtccggttc
    38161 gctgggacag cgatcacgta gtgctgctgg atgatgaaac ccaggcgatt gccagtgaga
    38221 ttgttcgtca cgatctgttc gaccgggtgc atatcggcct cgacttcttc gatgcctcta
    38281 tcaaccgcat tgccgcgtgg gtcattggta cacgcaatat gaaaaaagcc ctgctacgtg
    38341 cgttgctgga acctaccgct gaactacgca agctggaagc ggcgggcgat tacactgcac
    38401 gtctggcgct actggaagag cagaaatcgt tgccgtggca ggcggtatgg gaaatgtatt
    38461 gtcagcgtca cgatacgcca gcgggtagcg aatggctgga gaacgtgcgg acttatgaga
    38521 aagaaatttt gagtcgccgc gggtaaacac tgccggatgc ggcacaagtg ccttatccgg
    38581 cctacgggtc ggcaatagtt gtaggcctga taagacgcga cagcgtcgca tcaggcattg
    38641 attgccggat gcggcacgag tgccttatcc ggcctacaga tcgtacatcc ggcaacaggc
    38701 acgaacgtcc aattaacagg aacacagaac atgcaaaaca taacccagtc ctggtttgtc
    38761 cagggaatga tcaaagccac caccgatgcc tggctgaaag gctgggacga gcgcaacggc
    38821 ggcaacctga cgctgcgcct ggatgacgcc gatatcacac catatcacga caattttcac
    38881 caacaaccgc gctatatccc gctcagccag cccatgcctt tactggcaaa tacaccgttt
    38941 attgtcaccg gctccggcaa attcttccgt aacgtccagc ttgatcctgc ggctaactta
    39001 ggcatcgtaa aagtcgacag cgacggcgcg ggctaccaca ttctttgggg gttaaccaac
    39061 gaagccgtcc ccacttccga acttccagct cacttccttt cccactgcga gcgcattaaa
    39121 gccaccaacg gcaaagatcg ggtgatcatg cactgccacg ccactaacct gatcgctctc
    39181 acctacgtgc tggaaaacga caccgcggtc ttcactcgcc aactgtggga aggcagcacc
    39241 gagtgtctgg tggtattccc ggatggcgtt ggcattctgc cgtggatggt acccggcacg
    39301 gacgaaatcg gccaggcaac tgcacaagag atgcaaaaac attcgctggt gttgtggccc
    39361 ttccacggtg tgttcggttc cggaccgaca ctggatgaaa ccttcggttt aatcgacacc
    39421 gcagaaaaat cagcacaaat attagtgaag gtttattcga tgggcggcat gaaacagacc
    39481 atcagccgtg aagagttgat agcgctcggc cagcgtttcg gcgttacacc actcgccagt
    39541 gcgctggcgc tgtaaggagc aaacatgatc cgcaaagcct ttgtcatgca ggtaaacccc
    39601 gacgcccacg aagagtatca gcgtcggcat aatcccatct ggccggaact ggaagcagtg
    39661 ctgaaatctc acggtgcgca taactacgcc atctatctcg acaaagcgca taatctgcta
    39721 tttgccacgg tagagattga atctgaagaa cggtggaatg cggttgccag tacagatgtt
    39781 tgccaacgtt ggtggaaata tatgaccgac gttatgcccg ctaatgcgga taacagccca
    39841 gtcagtagcg aactgcaaga agtgttttac ctgccttaat gagttgcggt gccggaaaat
    39901 atccagcacc gccctttcat cagaagaagt atttaaagcg aacgcgtgcg ccgtaacggt
    39961 catcactatc gtcgccgtgt atcttatcgc cgtcgctatc cagaatggag taatacgtgc
    40021 cgaggtaaat attaaagttc tccatatcca tcacattagc gaactgataa gacgcatgaa
    40081 tggtgtgaat gttgtatgtt ccttcatcat caatccagca atcgttatca caaaccacgc
    40141 cactaaattc atcaatctta ttatgtgcat agatataacc cagctcgaaa cgtttccaca
    40201 gcgcgttaat ccctgccgtg aaatcttttt cattattagc atctaaatag gcggtattaa
    40261 gattaaccac gatgccattt tccggatcgg ttttcaggcc attccaggtc atactcatgc
    40321 cataaccggt acgatcggac tgatcgacaa aattaccctt gctatcggta taaccataag
    40381 cattatttac cacattcgct tccatcgctg cggaaacggt gaattcttcc gtcggcgacc
    40441 aggcaattac cggacgcaga taagcaacat ttttctgctg ttccatatcg cgtccatgat
    40501 aattaccgtc gttatataaa gatgttccgt cttccagtaa cgtgtttaat tcaaaatacc
    40561 agttatcgag ttgtttgctg acgaggaaat taccgccagc gttagaacgt ccgcggccct
    40621 ctttcatcat atagatataa ccgctgccat cgtcataaag atcgttcgca gtattaccgg
    40681 aatgttcaac aaaggtatcc tgattcagcg ggaacatatc gtaggcttca aaacggccta
    40741 ctttgacctt ccagtcgttt tctttgccaa agaagaaaac cgcatcatcg atatttactg
    40801 aaccgtgcat atcccccagc ggttgtgcgg agaacccagc gaaatagcca ttatccagtt
    40861 ttcgcatacc atcaaaacct aacagaatac gaccattgag attccactgt tcattagtgg
    40921 gatcgctatt cacatcagcg ttggtcattg ccagcaggcc atgattactt tccgcatcca
    40981 tattaaattc tacgtcaccg tagattttta aatcgccata ccctgaaagc aggagattag
    41041 gcggggtggc gtttttcttc tctgactccg tcgttgattg accgtttacc ggcgtgttgc
    41101 cctgtgcggt tttaatttcg cggatctcgg cggcttgttg tgtctgaagc tgtttaacct
    41161 gcccctctgc ttttgctgcc cgcatttctg ccgcttccag gcgttcttcc agcaatgcca
    41221 tgcgttgttc cagcgttaaa tgctgtgttt ttgccatacc ggtactacat gccaataata
    41281 aaccgatggc taaagataat gtcgattttt tcattattta cgttccctga aataaaggag
    41341 accctacgct ttccaaaagg aatacgtata tagagaaata taaggtgtgc actttcgaat
    41401 ttttaatcca acttcagcag gccgatggat agtatcccgc cgcataatat gagtaaatcc
    41461 gctgatttcc tggaacgtga ggaatataac aatagtgccg tttttaacta aatatcgtaa
    41521 aaaatattag atatgattca cgctgactca taagccacaa atataaataa cgcttaaaca
    41581 aattatctga gacat
//
LOCUS       DAZGJT010000032        40495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZGJT010000032 DAZGJT010000000
VERSION     DAZGJT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.032"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(110..598)
                     /locus_tag="ACHKFK_001885"
                     /pseudo
     CDS             complement(110..598)
                     /locus_tag="ACHKFK_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
     gene            complement(686..1519)
                     /gene="fdhD"
                     /locus_tag="ACHKFK_001886"
     CDS             complement(686..1519)
                     /gene="fdhD"
                     /locus_tag="ACHKFK_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO9052236.1"
                     /translation="MKKTKQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQMFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1713..4763
                     /gene="fdnG"
                     /locus_tag="ACHKFK_001887"
     CDS             1713..4763
                     /gene="fdnG"
                     /locus_tag="ACHKFK_001887"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:2298..2300,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9052237.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSTWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSIGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLAANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4776..5678
                     /gene="fdoH"
                     /locus_tag="ACHKFK_001888"
     CDS             4776..5678
                     /gene="fdoH"
                     /locus_tag="ACHKFK_001888"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO9052238.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5675..6310
                     /gene="fdoI"
                     /locus_tag="ACHKFK_001889"
     CDS             5675..6310
                     /gene="fdoI"
                     /locus_tag="ACHKFK_001889"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9052239.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            6307..7236
                     /gene="fdhE"
                     /locus_tag="ACHKFK_001890"
     CDS             6307..7236
                     /gene="fdhE"
                     /locus_tag="ACHKFK_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO9052240.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTTRIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(7460..7678)
                     /gene="yiiE"
                     /locus_tag="ACHKFK_001891"
     CDS             complement(7460..7678)
                     /gene="yiiE"
                     /locus_tag="ACHKFK_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9052241.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPMRKAKGGRQRDEAVGVEELCKQHKE"
     gene            complement(8074..8352)
                     /locus_tag="ACHKFK_001892"
     CDS             complement(8074..8352)
                     /locus_tag="ACHKFK_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052242.1"
                     /translation="MSTFNTRKDRDRNGKAKPHKHSGIPALTGKWRHPSMDCWVNYST
                     PGDHEWKRLYTTLRRRADDRRQCHRVMQGDDPEGIVWFPDCYPQIYYY"
     gene            complement(8711..9001)
                     /gene="nadS"
                     /locus_tag="ACHKFK_001893"
     CDS             complement(8711..9001)
                     /gene="nadS"
                     /locus_tag="ACHKFK_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO9052243.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(9002..9313)
                     /locus_tag="ACHKFK_001894"
     CDS             complement(9002..9313)
                     /locus_tag="ACHKFK_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9052244.1"
                     /translation="MLFIETEIFTEDVQKLLNDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            9542..10450
                     /locus_tag="ACHKFK_001895"
     CDS             9542..10450
                     /locus_tag="ACHKFK_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9052245.1"
                     /translation="MALEKGIAELVEEFIAAGRPSSRQQSITQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRIIAVQYRLAPEHTFPAAHDDAQRGAELVRQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPIWRNDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(10514..11455)
                     /gene="fabY"
                     /locus_tag="ACHKFK_001896"
                     /gene_synonym="yiiD"
     CDS             complement(10514..11455)
                     /gene="fabY"
                     /locus_tag="ACHKFK_001896"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO9052246.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(11500..11937)
                     /gene="dtd"
                     /locus_tag="ACHKFK_001897"
     CDS             complement(11500..11937)
                     /gene="dtd"
                     /locus_tag="ACHKFK_001897"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO9052247.1"
                     /translation="MIALIQRVTRASVTVEGEVTGKIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11934..12806)
                     /gene="yihY"
                     /locus_tag="ACHKFK_001898"
     CDS             complement(11934..12806)
                     /gene="yihY"
                     /locus_tag="ACHKFK_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO9052248.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12800..13399)
                     /gene="yihX"
                     /locus_tag="ACHKFK_001899"
     CDS             complement(12800..13399)
                     /gene="yihX"
                     /locus_tag="ACHKFK_001899"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO9052249.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPDD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(13498..14283)
                     /gene="csqR"
                     /locus_tag="ACHKFK_001900"
     CDS             complement(13498..14283)
                     /gene="csqR"
                     /locus_tag="ACHKFK_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9052250.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(14317..15213)
                     /gene="yihV"
                     /locus_tag="ACHKFK_001901"
     CDS             complement(14317..15213)
                     /gene="yihV"
                     /locus_tag="ACHKFK_001901"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO9052251.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVAKNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDAK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVAFSDHAAFSEPGLARLTGVKEMASALKQAQMLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATGGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            15381..16277
                     /gene="yihU"
                     /locus_tag="ACHKFK_001902"
     CDS             15381..16277
                     /gene="yihU"
                     /locus_tag="ACHKFK_001902"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO9052252.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLCVFDVNTEAVQHLVDKG
                     ATPAANPAQAAKGAEFIITMLPNGDLVRHVLFGENGVCESLSSDALVIDMSTIHPLQT
                     DKLIADMQARGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            16301..17179
                     /gene="yihT"
                     /locus_tag="ACHKFK_001903"
     CDS             16301..17179
                     /gene="yihT"
                     /locus_tag="ACHKFK_001903"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO9052253.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKTLSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNEMCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLSASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            17195..18436
                     /gene="yihS"
                     /locus_tag="ACHKFK_001904"
     CDS             17195..18436
                     /gene="yihS"
                     /locus_tag="ACHKFK_001904"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO9052254.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKKSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWDYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGLAPAVAAGLLDINAK"
     gene            18503..19405
                     /gene="yihR"
                     /locus_tag="ACHKFK_001905"
     CDS             18503..19405
                     /gene="yihR"
                     /locus_tag="ACHKFK_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO9052255.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHGSKAAIHGLLAWRGWQI
                     SELSATSVTLTAFLPPSYGYPFMLASQVVYSLNARTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAIDEHANPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKTHRLFFNIHGQHN"
     gene            19466..20341
                     /locus_tag="ACHKFK_001906"
     CDS             19466..20341
                     /locus_tag="ACHKFK_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO9052256.1"
                     /translation="MDFFTLFSPDGVMLETNVVCIVIALMFLYTFVGICAGFGGGLTT
                     MPLVTLMLPVKMATPLSVIVGTATALYATWLSRKETDWRSAMVLIIFSFLGIPVGLYA
                     LSYLPDHFMKVGLGGFLILYSFYSMFILRLPIYDRSWIAAPIGAVAGALGAAFSTNGP
                     PMVIYGMLRNLGPAAFRGTLNAFFTANNIAVIGGLATSGILTVSIFKLVLFCIPTMIL
                     GSLVGQYVHKRISVKVFRVMVFLLLIASGSMLIKGALGIPAAAALLPPFTLLVILQLA
                     FGKKVAAIRRAEESK"
     gene            20447..22483
                     /gene="yihQ"
                     /locus_tag="ACHKFK_001907"
     CDS             20447..22483
                     /gene="yihQ"
                     /locus_tag="ACHKFK_001907"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO9052257.1"
                     /translation="MDTPRPQLIDFQFHQNNDSFTLRFQDRLILIHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDHGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWARDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNKEGVQFLAYI
                     NPYVASDKDLCEEAAKRGYLAKDAAGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAQTYTLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            22529..23914
                     /gene="yihP"
                     /locus_tag="ACHKFK_001908"
     CDS             22529..23914
                     /gene="yihP"
                     /locus_tag="ACHKFK_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052258.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            23957..25360
                     /gene="yihO"
                     /locus_tag="ACHKFK_001909"
     CDS             23957..25360
                     /gene="yihO"
                     /locus_tag="ACHKFK_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052259.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLSIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAIPAALIAT
                     LQFIATTFSLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTTP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPLTVKCFGKKQVYLAGMVLWAVGDILNYCWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYIPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEIETEEKTASVTL"
     gene            25428..26120
                     /gene="ompL"
                     /locus_tag="ACHKFK_001910"
     CDS             25428..26120
                     /gene="ompL"
                     /locus_tag="ACHKFK_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO9052260.1"
                     /translation="MKNINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRMG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFTPWFNLTVRNRYNHNNYSSTDLNGELDNNDTYEVGTYWNFKITEKF
                     SYTFEPHYFMRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(26222..28045)
                     /gene="typA"
                     /locus_tag="ACHKFK_001911"
     CDS             complement(26222..28045)
                     /gene="typA"
                     /locus_tag="ACHKFK_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO9052261.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            28418..29827
                     /gene="glnA"
                     /locus_tag="ACHKFK_001912"
     CDS             28418..29827
                     /gene="glnA"
                     /locus_tag="ACHKFK_001912"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO9052262.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            30001..31050
                     /gene="glnL"
                     /locus_tag="ACHKFK_001913"
     CDS             30001..31050
                     /gene="glnL"
                     /locus_tag="ACHKFK_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO9052263.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRITESIHKVAERVVTLVSMELPNNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            31062..32471
                     /gene="glnG"
                     /locus_tag="ACHKFK_001914"
     CDS             31062..32471
                     /gene="glnG"
                     /locus_tag="ACHKFK_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO9052264.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESNVPE
                     STSHMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLATALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            32583..32693
                     /gene="yshB"
                     /locus_tag="ACHKFK_001915"
     CDS             32583..32693
                     /gene="yshB"
                     /locus_tag="ACHKFK_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO9052265.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(32926..34299)
                     /gene="hemN"
                     /locus_tag="ACHKFK_001916"
     CDS             complement(32926..34299)
                     /gene="hemN"
                     /locus_tag="ACHKFK_001916"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO9052266.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEQQW
                     DLLFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(34488..34997)
                     /gene="yihI"
                     /locus_tag="ACHKFK_001917"
     CDS             complement(34488..34997)
                     /gene="yihI"
                     /locus_tag="ACHKFK_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO9052267.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(35116..35282)
                     /locus_tag="ACHKFK_001918"
                     /pseudo
     CDS             complement(35116..35282)
                     /locus_tag="ACHKFK_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35579..36211
                     /gene="yihA"
                     /locus_tag="ACHKFK_001919"
     CDS             35579..36211
                     /gene="yihA"
                     /locus_tag="ACHKFK_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO9052268.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(36593..39379)
                     /gene="polA"
                     /locus_tag="ACHKFK_001920"
     CDS             complement(36593..39379)
                     /gene="polA"
                     /locus_tag="ACHKFK_001920"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO9052269.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQPPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAARPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKEWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTRQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            39743..>40495
                     /locus_tag="ACHKFK_001921"
     CDS             39743..>40495
                     /locus_tag="ACHKFK_001921"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9052270.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLS"
BASE COUNT         9732 a        10462 c        10169 g        10132 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcaat aagttggagt cattaccgac ttcgtgacat
       61 atccgtgctc atcacaaacc cgccccgcga ttacaattaa ctgaaatatt tcatcgacca
      121 tttcgttgta atcgcataac gctcgtggaa atgagtcttc ggccatccag cttgagtttg
      181 cgtcctgaag ttgctcttta tcccagtcag agtacgggac tttatctctg atgcggcgaa
      241 cgtatttttt agcctcgagt tgatactggc ctgccgaatt aataaacgag aaattcatgc
      301 cgcttttatc cgcttctttg gcttgcgcca ccagcgtttc cagttcagac actttcttca
      361 ttgccgcttc tgcatctaac ttatcttgcg atatcaggtt attaatttgt ttcgctgaaa
      421 tcatgactgc cagagagtag tagtggaatg ttttcccttt cgcaaacgca tcgtctttat
      481 aattttcctg ggtgtaatat ttatccattt cgttaatggt attacccaac gctacggcag
      541 catcaatata actcaccgcc acgccatcaa taggttgcag cgcaggcgtt aacgccacta
      601 cacgcgtttt gtttatgaaa aaataacaaa attcacgaaa tgaataagtc ctgtaattta
      661 atatatatcg caggacttat tttaattaat tgctcaaacg ctgcggatgg gtataaaccg
      721 ttgcccgacc cggtttacaa aagcccacca gcgtcagatt acagcgctca gccacttcta
      781 cagcaagtgt ggtcgcggca gacaccgcaa acaaaatctc tacgccgcac atcgccgact
      841 tttgcaccat ttcataactg gcacggctgg aaaccagcac cgcaccttgc tgccagcttt
      901 ccccttcttg tgaccggcgg cctaacagtt tgtccagcgc cacatggcga cccacgtctt
      961 catgcccgcc gaccagttcg ccagatggca acatccatgc agcggcgtga gtacaaccag
     1021 tcagttgccc cactggctgg aaatcattga gatgacgtaa tgcatcatcc agtttgttga
     1081 gatcaaacat ctgggtgaac ggcagcggct gcaccggttt accgatatcg ttaagctgtt
     1141 ctacaccgca tacgccacag cccgtacgtc ccgccagcgc ccggcggcgc tccttcaacc
     1201 ccataaagcg gcggctggaa agctcaattt gtacttccag gccattacag gaaggaacga
     1261 catccatgcc gaagatatcg cgcgggcttt cgataattcc ttcggaaagc gaaaaaccga
     1321 gcgcaaagta ctcaaggtct ttgggcgacg ccatcatcac cacatgcgaa atgccgttgt
     1381 agaccagcgc aacgggaact tcttccgcca cctcatctaa ccgtgggtgt tgtaaatcat
     1441 cacgccgcca taactcaatt tgacgaacac ctgtgatatt tgtcacattc tcgatttctt
     1501 tttgctttgt ttttttcact ttctattaac cagataagaa cacacacacc aacattatgg
     1561 tattctgtta caaatccttc ctggatggag ggaaattgag ccaattctgg acctttgcgg
     1621 ccccttccgc aaagaaaaat aactcccact ccctgcacac gcagcaagcg aatgtaaatg
     1681 ggacgtgaca atgtcgaaac aaggagcaat ccatgcaggt cagcagaagg cagttcttta
     1741 agatctgcgc tggcggtatg gcaggcacca cggcagcggc actgggtttt gcacccagcg
     1801 tagcactcgc ggaaacccgg cagtataaac tgctgcgcac ccgcgaaacc cgtaatacct
     1861 gcacctattg ttccgtaggc tgtgggctgt tgatgtacag cctcggtgac ggagcaaaaa
     1921 acgccaaagc atctatcttc catatcgaag gtgacccgga tcacccagtc aaccgcggag
     1981 cactttgtcc gaaaggcgct ggcctggtgg atttcatcca ctccgaaagc cgtctgaagt
     2041 ttccggaata ccgtgcgcca ggttctgata aatggcaaca aatcagttgg gaagaggcgt
     2101 ttgatcgcat cgccaaactg atgaaagaag accgcgatgc taactacatt gcgcaaaacg
     2161 ccgaaggcgt gactgttaac cgctggctct ccaccgggat gctgtgtgct tccgcgtcga
     2221 gtaacgaaac cggctattta acgcaaaaat tctcccgcgc gctgggtatg ctcgcggtcg
     2281 acaaccaggc gcgtgtctga cacggaccaa cggtagcaag tcttgctcca acatttggtc
     2341 gcggtgcgat gaccaaccac tgggtcgaca tcaagaatgc caacctcgtc gtggtgatgg
     2401 gcggtaacgc cgctgaagct cacccggtcg ggttccgctg ggcgatggaa gccaaaattc
     2461 acaacggcgc gaagctgatt gtgatcgatc ctcgctttac acgtacagcg gcggtggctg
     2521 actactatgc ccctatccgt tccggtactg acattgcttt cctgtcaggc gtattgctgt
     2581 acctgctgaa caatgaaaaa ttcaaccgcg aatacaccga agcctatacc aacgccagcc
     2641 tgatcgtgcg tgaggattac ggctttgaag atggcctgtt caccggctac gacgcggaaa
     2701 aacgcaagta cgataagtcc acctggactt acgaactgga tgaaaacggc ttcgccaaac
     2761 gtgatactac cctgcaacat ccacgttgcg tgtggaactt gctgaaacag cacgtttccc
     2821 gttatacgcc agatgtggtt gaaaacatct gcggtacgcc aaaagacgcg ttcctgaaag
     2881 tctgcgaata catcgccgaa accagtgctc acgataaaac agcgtcgttc ctctacgccc
     2941 tcggctggac gcaacactcc attggtgcgc aaaacattcg tacgatggcg atgatccagc
     3001 tgctgctcgg caacatgggg atggctggcg gcggcgttaa cgccctgcgc ggtcactcca
     3061 atattcaggg gctgacggac ctggggctac tgtcgcagag tctgccaggt tacatgacgc
     3121 tgccaagcga gaagcagacc gatctgcaaa cttacctcgc tgccaacacg ccaaaaccac
     3181 tgctggaagg ccaggtcaac tactggggca actacccgaa attcttcgtc tcaatgatga
     3241 aggcattctt tggtgataaa gcgacggcgg aaaatagctg gggctttgac tggttgccga
     3301 agtgggataa aggctacgac gtcctgcaat acttcgagat gatgaaagag ggcaaggtca
     3361 atggctatat ctgccagggc tttaaccctg ttgcctcatt cccgaacaaa aacaaagtga
     3421 tcggctgtct gtcgaagctg aaattcctcg tgactatcga cccgctgaac actgaaacct
     3481 ctaacttctg gcagaaccac ggtgagctga acgaagttga ctcgtcgaag atccagaccg
     3541 aagtgttccg tctgccatcg acctgctttg cggaagagaa cggttcaatc gttaactcag
     3601 gtcgctggtt gcagtggcac tggaaaggtg cggacgcccc tgggattgcg ctgactgacg
     3661 gcgagatcct ctccggtatc ttcctgcgct tgcgcaagat gtatgccgaa cagggtggcg
     3721 cgaacccgga ccaggtgctg aacatgacct ggaactacgc catcccgcat gagccgaagt
     3781 cagaagaagt ggcgatggag agcaacggta aggcgctggc cgatattacc gatccggcaa
     3841 ccggtgcggt tatcgtcaag aaaggccaac aacttagctc gttcgcccaa ctgcgcgatg
     3901 acggtaccac ctcctgtggc tgctggattt tcgccggtag ctggacgcca gaaggtaacc
     3961 agatggcacg ccgtgataac gccgatccgt ctggcctcgg taacacgctg ggctgggcat
     4021 gggcatggcc gcttaaccgc cgcattctgt ataaccgcgc ctccgcagat ccgcagggta
     4081 acccgtggga tccgaagcgt cagttgctga aatgggacgg cactaagtgg accggctggg
     4141 atattccgga ctacagcgca gcgcctccgg gcagcggcgt agggccgttt atcatgcagc
     4201 aggaaggcat gggacgtctg tttgccctcg ataagatggc ggaaggtccg ttcccggaac
     4261 actacgagcc gtttgaaacg ccgctgggaa ctaacccgct gcatccaaac gttatctcga
     4321 acccggctgc gcgtatcttt aaagacgacg ccgaagcatt gggtaaagcc gataagttcc
     4381 cgtatgtcgg aaccacctat cgtctgaccg agcacttcca ctactggacc aaacacgcgc
     4441 tgttgaacgc aattttgcaa ccagagcagt ttgtggaaat cggggagtcg ctggcgaata
     4501 aacttggcat tgcccagggc gatactgtga aagtctcctc caaccgtggc tatatcaaag
     4561 ccaaagcggt agtgaccaaa cgtattcgca cgctgaaagc aaacggcaaa gatatcgata
     4621 ccatcggtat tccgattcac tggggctatg aaggtgttgc gaaaaaaggc tttattgcca
     4681 atacgttgac gccattcgtc ggtgatgcga acacgcagac gccggagttt aagtccttcc
     4741 ttgtgaatgt ggaaaaggtg taacggagac gacatatggc ttatcaatcg caagatatca
     4801 ttcgtcgttc cgcgactaac ggtctgaccc ccgcgcctca ggcgcgggac ttccaggaag
     4861 aagtggcgaa actcatcgac gttaccacct gtatcggctg taaagcctgt caggtggcgt
     4921 gttcagagtg gaacgacatt cgcgataccg tcggcaataa cattggggtg tacgacaacc
     4981 ccaatgattt aagtgccaaa tcgtggacgg tgatgcgatt ctcggaagtg gagcagaacg
     5041 acaaactgga atggctgatc cgcaaagacg gctgtatgca ctgttccgat ccaggctgcc
     5101 tgaaagcgtg cccggcggaa ggggcaatta ttcagtatgc caacggtatc gtcgacttcc
     5161 agtccgagca gtgcatcggc tgcggttatt gcattgcggg ctgtccgttc gacattccgc
     5221 gcctcaaccc ggaagacaac cgcgtctaca aatgtacgtt gtgcgttgac cgcgtggtgg
     5281 ttgggcaaga accggcctgc gtgaagacct gcccaacggg cgcgattcac ttcggtacga
     5341 aagagtcgat gaaaacgctg gcgagcgagc gcgtggcgga gctgaaaacc cgtggttacg
     5401 acaatgcggg tttgtacgac ccggcaggcg tcggtggtac acatgttatg tacgtgctgc
     5461 accatgctga caagccaaat ctgtatcatg gcttgccgga gaacccggaa atcagcgaaa
     5521 ccgtgaaatt ctggaaaggc atctggaaac cgctcgcagc tgttggcttt gcggctacct
     5581 tcgcagccag tatcttccac tacgtgggtg tcggtccgaa ccgtgcggat gaggaagaga
     5641 ataatctgca cgaagagaaa gacgaggagc gcaaatgaaa cgacgtgaca ccatcgtgcg
     5701 ctacaccgcg ccggaacgta tcaaccactg gatcaccgcc ttctgcttca tcctggcggc
     5761 ggtgagcggg ctgggctttt tgttcccgtc cttcaactgg ttgatgcaaa tcatgggcac
     5821 accgcagctg gcgcgcattc tgcacccgtt tgtcggcgtg gttatgtttg cctcgttcat
     5881 catcatgttt ttccgttact ggcatcacaa cctaatcaat cgggatgata tcttttgggc
     5941 gaagaatatt cgtaagatcg tcgtcaacga ggaagtaggt gacaccgggc gttataactt
     6001 cggtcagaaa tgcgttttct gggcggcgat tattttcctg gttctgctgc tggtgagcgg
     6061 cgtgattatc tggcgtcctt attttgcgcc tgctttctca atcccggtga tccgattcgc
     6121 gttaatgctg cattcatttg ccgcagtggc gttaattgtg gttatcatgg tgcatatcta
     6181 cgccgccctt tgggtgaaag gcacgattac cgccatggtg gaaggatggg tgaccagcgc
     6241 ctgggcgaag aaacatcacc cgcgctggta ccgtgaggtc cgcaagacaa cggaaaagaa
     6301 agctgaatga gtattcgcat aatcccgcaa gatgagctgg gttcgagcga gaaacgtacg
     6361 gcggatatga ttccgccgtt attgttccct cggctcaaga atttatacaa ccgccgcgcc
     6421 gagcgtctgc gcgagctggc agaaaataat ccgctgggtg attacctgcg ctttgctgcg
     6481 cttatcgccc acgcccagga agtggtgctg tacgaccatc cgctggagat ggatctgacc
     6541 acgcgcatta aagaagccag cgcacagggc aagcccccgc tggatattca cgttctgccg
     6601 cgtgataagc actggcaaaa gctgctgatg gcgctgattg ctgagctgaa acctgaaatg
     6661 agcggcccgg cgctggcagt gattgagaat ctggagaagg catcgactca ggagctggaa
     6721 gatatggcca gcgcactgtt tgcctctgat ttctcgtccg tcagcagcga taaagcgccg
     6781 tttatctggg ctgcgctgtc gctctattgg gcgcagatgg ctaatctgat ccccggcaaa
     6841 gcccgcgctg aatacggcga acaacgtcaa tattgcccgg tatgtggctc tatgccggtg
     6901 tccagcatgg tgcaaattgg caccactcag ggtctgcgtt acctgcactg caacctgtgt
     6961 gaaactgaat ggcacgtagt acgcgtaaaa tgcagcaact gtgaacagag cggcaaactg
     7021 cattactggt cgctggatga cgaacaggcc gcgattaaag ccgaaagctg cgatgactgc
     7081 ggcacttacc tgaagattct ttatcaggaa aaagaaccga aagttgaagc cgtggcagat
     7141 gacctcgcct ctctggtact ggacgcgcga atggagcaag aaggctatgc ccgcagttcc
     7201 atcaacccgt tcctgtttcc gggtgaaggg gagtaatctc tgacgttttc cgggtggcgc
     7261 tggtcgcccg gattacagct catagggtgt gacgacacct gctattcccg caaaatctct
     7321 cgtatttcgc gaggcgtttt ccagatatga tgtaacagtt gcagttatta taagttcaag
     7381 taaaacaacc ttgtattaat accagaataa ggtcatctgg ttttttgttt ttatattcaa
     7441 gaatgaaaaa atctttttgt cattccttat gctgcttaca tagttcttca acaccaactg
     7501 cctcgtccct ttgcctgccg ccttttgcct tacgcatcgg aaattgctgc cgctccagat
     7561 attgctctac cgcttcgcgt aaaattgcag atcgtgaaac cttacgccag cccctgagtt
     7621 tattcagccg tttaattgcc tcttctgata aatgaagaaa aaccgtattc atcgccatta
     7681 ccaccacctt acatatatta ctcattaatg tatgtgcgaa aattacgccg gaaagttaat
     7741 taacgaaagc ggattttttc atattgtttt gtttttgcga gacggcatcc aaagataatg
     7801 taacagtttc agttatttca attgttactc aaaaatggtt tattactttt aattaagcgg
     7861 gggcacatct acagaaaaga aacaacaaat aataaggatg aattatggat gctataaata
     7921 ttttatattc aagaatattt tttgattggg tcttcatttg cttcatcagg atggtgtacc
     7981 cgaaaaagtc agcagcttta ccgcaccagg gtttaaggtg aaatctacca ttcataacga
     8041 ccggtcagtg ccgcaccaac cggtctttaa ttgttagtag tagtaaattt gtggataaca
     8101 gtccgggaac cagacgattc cttccggatc atccccctgc attacccgat gacattggcg
     8161 tcgatcatct gcgcgacggc gaagcgtagt atagagccgc ttccattcat gatcgcccgg
     8221 tgtactgtag tttacccaac aatccattga agggtgacgc catttacctg taagtgctgg
     8281 gatccctgaa tgcttatgcg gcttcgcttt accattacga tccctatctt tacgcgtatt
     8341 aaacgtggac atttttagtc tcctgttaag agttctaaaa atgtgcttcc ttaaaatagt
     8401 tcatcatttt cccttaattc gcatagcgtg cttttgcacg aatttccgat acgcggggac
     8461 cattatgata gcgtcgatgc ccacacatcc aacaagaaca aatacaggga gtacgaaagc
     8521 aaatacctga tgcttttttg cttccatcgc ctgcagtgcg ataacgacag cggatttttt
     8581 ttaatcgcac catatgatgg cgacgttcag cacgattacg ggtcatagca aaactcctga
     8641 gcaggcggca ctatgccgct ttcataggag ttctgaatgc tggtgctttc tgtatggcat
     8701 aaccatgcct ctaacggtta agtgcttgca gcacatgttg cggatcattg gcaatcgctc
     8761 gcagcaaagc ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg
     8821 tgtctacgct tacggaaata agatcggcaa atttagattg tgacaaccct gaagcgcggc
     8881 gtatttcttt gattttcata gcatcaactt caaaagtccg tgaaggctgt cgcgttcctt
     8941 caataatttc attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca
     9001 tttaccacct cgtatttaac aaccggagca ttgctttttc ctggggagac agatcatctt
     9061 taataccctt acgataaata agcagtaatc tgatctcata atgtttaacc tgatgaaagt
     9121 aaataaccct gacaccagca cgtttcccct ttcctcctga gacccatcga acctttctta
     9181 acccaccagt ttcaggtatt acctcaccat agtcaggatt caaggccagg aaaaactgaa
     9241 aacgactgaa ttcatcgtcg ttcagcagtt tttgaacatc ttcagtaaaa atttccgttt
     9301 cgatgaagag cataattata cgccattggc ttagagtaac aatccttttt gtacgtcatt
     9361 gacgtaccat tgaaaccact agcttactga tagtgctcca gcaaaaccac gtctctgccg
     9421 ggtttgtctt tacatttgcg aggcgctttc cagatttttc acgcccgtga atttccctgt
     9481 atccatccag caaaaacggg tgtataaaac tgtacattca tacagcaaaa tgagttctac
     9541 tatggcactg gaaaaaggaa ttgcagagtt agtcgaagag tttatcgctg cgggtcgccc
     9601 ctcatcgcgc caacaaagta ttacgcagcg cagagagggc tatatcgcca gcgctgtcct
     9661 tgcgggtgag acagaaacgc gggtagatat tcagactatc gaacttgaag ggatgacctt
     9721 acgaatagtg tcaccgctga atgccccgac attactcccg acgataattt attactacgg
     9781 tggctgtttt gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc agttagccta
     9841 ttacgggcaa tgtcggatta ttgccgtcca gtatcggctg gcaccggaac atactttccc
     9901 cgccgcccat gatgatgcac aacgaggtgc agaattagtc cggcaacacg ccgaacgact
     9961 cggtgttgat aaacaacgaa taactcttgc cggtgacagt gcgggagggc atctggcgct
    10021 ggtaactgct ttgcgattga agcgtgctgg cgagtggcaa ccagcacaac tcatcttaat
    10081 ttatcctatg cttgatgcga ctgcgcattt cgaaagctac atacgcaatg gtcacgatta
    10141 catcatcaca cgcgacactt tactgagcgg ctttgagatg tacctgccag gcattgaacg
    10201 caggcatcct gaagccagcc ctatttggcg caatgatttc aatggcttgc cacctgtaca
    10261 tatcattacc gctgaatacg acccactttg cgatgaaggt gaagcgttgt atcaccgcat
    10321 gacagggcaa ggagtgcaat gcacctgcca acgctacctt ggcgttatcc acggattttt
    10381 tcagttaggt ggtatcagtg aggctgcgcg atcggcgcta cgggatgttg cctggagagc
    10441 gggaaggtaa ctccggacca gggcccagaa aaggttttca gattgaaaac cttttcagtc
    10501 aagcaaaacg cccctactct tcttcgttcc cgccctcttc atacgggcca aatggcttcg
    10561 cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt
    10621 ctacttgcat ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccactta
    10681 aggcaccaag gtcggctacc gcatggggtt ttccgctaat cggcttgctg tagcggatat
    10741 gcgcatccgc cagaataatc gttccgccga ggtggcgttc gcgcagcatc agccagataa
    10801 gcccccaacc ggtgagcgtt gccagcgaga ataaactccc ggcaaacagc gtgtgatgcg
    10861 gattctgatt gcctgtttcc ggcatggtgg tgataaattt ttgcccggta tattgctgaa
    10921 tgcgcacacc cattttttca ctcagcggga tgtgttcata ccacgcttgc tggagctggg
    10981 cgcaccagtc gccgcgatga agaatatcat ccagagtggc aacaggtttt atcatcagaa
    11041 aatggcgaat cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca aaccccagct
    11101 tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt cacgcgctta acgccttcct
    11161 gacgtgccac cgactccagg gtcatcgcca tcagcgtgcc taaccctttg tcctgtacgt
    11221 cgggatgaac agccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc
    11281 ctaccgctac cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc
    11341 acgcgtcgcg ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa
    11401 actgatagta acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga
    11461 tactctctct tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt
    11521 cacggggcca tcattgacca gcgatacctg catatccgca gcgaagcgtc ctgtttgcgt
    11581 gttcatctct tgctgacggc agcgttcgac gaaatagtca tataacgcct ctgcgcgatc
    11641 cggtgatgcg cctttggaga aacttgggcg catcccccgt tcggtatctg cggcaagggt
    11701 aaactgggaa accaccagta cactgccgcc cgcctgttgc acgttgagat tcatcttgcc
    11761 ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg cacagacggt ttgctttctg
    11821 ttcgtcgtca tccttttcga cacccaataa caccaaaagt cccgcgccaa ttttacccgt
    11881 cacttctccc tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt
    11941 tcgtcgtctt cttcttgttc agcagcttgt tttaatttgc ggtattcccc gagagtgaca
    12001 gtaatttccg cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg
    12061 gggatcaccg ccagcacacc gtaaatgagc tgatatgatg ggaacatggt gatataaagc
    12121 gcgaaacctt tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc gacaatcgcg
    12181 tcgcggttag gtacacggat ggtaggaacg atgctgtaca gcagccagaa agagatccac
    12241 gacaacagca gcggaaaaat acgcaacaca ttatcgatga cagtattgag atcgctcgcc
    12301 cagcgcagag agagcagata ggagctgatc gccagactgg cccctgccaa cagcggcccc
    12361 agcgttaaaa tcatccagta cacggcaaac gagtaaattt tgggtcgcgc tcgtttactg
    12421 cgccagatgg tattcaacgc gctatcgatg gagtacatca acaataacgc cgtgacgatc
    12481 agcccgcacg ccccgacggc ggtcattttg ttggaattgg cgacaaactg ttcgatatat
    12541 cgctggataa catcgccagt agcaggcaga aagttggcaa aaataaagtg acgcaactga
    12601 atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc
    12661 ggcactaatg agagcaacga cacataggca aggttacctg ccaaggttgt catgttgtcc
    12721 tcatcaatgc gttgccagag tagttttagc caggcccata gtggacgggt acgatgcctg
    12781 gctttgtcct gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt
    12841 tatctttcac cagaatacta gtaatgccta gctgattagc tccttctata ttatcggcgt
    12901 tatcgtcgaa aaagaccgta tcgtctggtg aaaaaccttc tgcctgcaaa acatgctgat
    12961 aaattcgtgc ttcaggtttg cgcataccca agtcttgcga cagatagata tggtcagcag
    13021 catcacggat ttccgggtat tcctccggcc agaaggtagt atgcaggcgg ttagtattgg
    13081 aaagcaccac cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg
    13141 ggcgcagcgc gacaaacacc gcctgccagc cgtgagagaa ctgctcgtag cttagcggta
    13201 gagccatctc atgacacagc gcctctgcga acgcttcgtc gctaatttcc ccacgctcat
    13261 gctgatgaaa cgcctcgccc atgtggaaac tcttcttaag cgtagccagt ggaacacgcg
    13321 ttaaatcgct ccaggctccc agcacacggt taaagtcgat atcgacaatc acattaccta
    13381 aatcaaagat atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg
    13441 aaaggatggg tttggctatg tagtcaggta tgaagccagg tcaacggtca caaaattcta
    13501 cgcgtcttcc tggggaagga tgatttcaat ttggctgtct tgtaagcgtg attttagcgc
    13561 agcgggcggc agctcgtcgg taaagagcgc agtgacctgt gccacattac caatttcaac
    13621 cgccgccgaa gcatgatact tagtgtgatc ggcgaccagc agaatatttc tcgcgtgcgc
    13681 catcatcgtt ttcaccacgt tagcttcgtt aacatcaaac tccatcaacg cgccatcgct
    13741 ctcaattgcg cctacacttg ttaccagata atcggcgcga aaatcggcca caaaggacgc
    13801 cgctgaaggg ccaatgatcc cactgttatg ggaacgcagc gttccgccag gcaccatcac
    13861 ttcaaagcgc gggttgtggt aaagaatatg cgccacacgc aggctgttgg tgattatccg
    13921 caaatggtta tggttaagta acgcccgggc aacatgctca acggtcgtac caatggtgat
    13981 aaatattgtt gaaccatcag gaatatagtc tgccaccgct tcggcaatcg cttttttttc
    14041 ttcggtttgc gaaacctcac gctgctcgaa cgccgtatta acgacgctgg aagcccgacc
    14101 cgcgccaccg tgatggcgcg taatcagtcc ttgctcgctt aatttacgaa tatcccggcg
    14161 gaccgtctgc gtggagacat ccagcagatt tgccagctca tcaatattca tatacccacg
    14221 ctcggcgatc agcatgagga gttggtcgtg ccgcgggtta ccggttagtt cggtaaggct
    14281 catgaaaatt cctcgaaaaa ccatcaccct ggcattttat acaaaaagtg acaaaaaaga
    14341 tcgggtttga tcacagtcag ggatcccggc tcgtccaccg ggacgcgtgc atttcaacgc
    14401 cgctactccg ctggcgaagc ggactgattc cgctaaatcc ccacctgttg ccagcgccac
    14461 cgccaatgcg ccgtgaaaaa catcacccgc acctgtggta tctaccacat caactttgaa
    14521 ggccggttga tgctgtcgcc cgccattctc cagccagtca cagcctgcac tgccctgggt
    14581 cacatagaca tgaccatttg tgagcatttg tgcctgtttt agggcactcg ccatctcttt
    14641 cacgcccgtt aagcgcgcca gacccggttc tgaaaaagct gcatgatcgc tgaacgccac
    14701 cagttcgctg atatcctgcg gcgtaatatc gccgtccagc acggtcatca cgcccgcctg
    14761 ccgcgccaga gtgaaggctt ttttcgcgcc gtcgtgccag cgtacatctg ccagcaccac
    14821 gtcccactga gagaaatcaa tttcctccaa ccactctgca tcaggcagca ggtctgggct
    14881 gggatagtta atgatgatgc gctcaccttt ggcatccacc atgatggcgg attgcgaaga
    14941 tttcgcctgg ttataccgtt tggtgtaacg ggtgttaaca ccccaggatt ccagctctgc
    15001 cagcaggctg ttgcccgtgt cgtcgtcacc gacgcgacca ataaaatcga cctgcgcacc
    15061 tagtctggcc gccgcaactg ctgctgtagc cgctggcccg ccgccaactt ccgtataatt
    15121 tttcgccacg tatttaccgc tctccgtcgg caatccttcc acgtaataga tgcgatccat
    15181 cacggtaatt cctacacaag caacacgaat catggtcatt ccttaagcat tttgggatgc
    15241 gttcatttta atttcatcaa ttgtttaaaa agtgacggct gtcaattttt tgacgataaa
    15301 ttacaaatac gatcaaaaac agacaagcaa ggcatgtcat aaatgacaaa aagtgacatc
    15361 actgtattca ggagagagtt atggcagcaa tcgcgtttat cggtttaggg caaatgggtt
    15421 cgccaatggc gagcaattta ttgcagcaag ggcatcaact ttgcgtcttc gatgtgaata
    15481 ccgaagccgt acagcatctg gtcgacaaag gggcgactcc cgctgctaac ccagcgcagg
    15541 ccgctaaagg tgccgaattt atcattacca tgttgccgaa tggcgatctg gtgcgtcacg
    15601 tgttgttcgg tgaaaacggt gtctgtgaga gcttatcttc cgatgcgctg gtcattgata
    15661 tgtcgaccat ccacccgctg caaaccgata aattgattgc tgatatgcag gccagaggct
    15721 tcagcatgat ggatgttccg gtaggccgta cttccgcaaa tgccattacc ggtactctgc
    15781 tactgctggc tggcggcact gctgaacaag ttgaacgcgc cacgccgatc ctgatggcga
    15841 tgggcagcga gttgatcaac gcgggcggtc cgggcatggg gatccgcgtt aagcttatca
    15901 acaactacat gagcatcgcg ctcaatgcgt tgtcggcaga agccgccgtt ttgtgcgaag
    15961 ccctcaatct tcccttcgat gttgccgtca aggtaatgag cggtactgcc gccggtaaag
    16021 gccacttcac cacttcctgg ccgaacaaag tcctcagcgg agatctttct cccgccttca
    16081 tgatcgatct tgcccataag gatcttggca tcgcccttga tgtcgccaac cagttgcatg
    16141 tgccgatgcc actgggggcc gcctcacggg aggtttatag ccaggcgcgc gcagcgggtc
    16201 gcggtcgcca ggactggtcc gccattctgg agcaggtccg tgtcagtgcc gggatgactg
    16261 ccaaagtaaa aatgtaacga ctggataaag gaataaatga atgaataagt acaccatcaa
    16321 cgacattacg cgtgcatcgg gcggttttgc catgctggcg gtcgatcagc gcgaagccat
    16381 gcgcatgatg tttgccgcgg ctggcgcacc cgctccagta gccgatagcg ttttaactga
    16441 tttcaaagtt aacgccgcga agactctttc accttatgca tcggcgattc tggtagatca
    16501 acaattctgc taccgccagg tggttgagca aaacgcgatt gccaaaagtt gcgccatgat
    16561 tgtcgccgcc gatgagttca ttcctggcaa cggtattccg gtagatagcg tggtcattga
    16621 ccgcaaaatc aatccgctac aaatcaaaca agacggtggc aaagccttaa aactgctggt
    16681 gctgtggcgt agtgatgaag atgcgcagca acgcctggat atggtgaaag agtttaacga
    16741 aatgtgccac tcacacggtc tggtaagcat cattgagcca gtcgtccgcc caccgcgtcg
    16801 tggcgataaa ttcgatcgcg aacaagcaat catcgatgca gccaaagagc tgggcgacag
    16861 tggcgctgat ctctacaaag ttgaaatgcc gctttacggt aaaggtccgc aacaagagct
    16921 tctcagtgct tcacaacgtc tgaatgacca tatcaatatg ccgtgggtga tcctctcttc
    16981 cggtgtcgac gaaaaactgt tcccgcgtgc cgtacgcgtg gcaatgacag cgggtgcatc
    17041 gggcttcctg gctggtcgtg ccgtctgggc atcggtcgtc ggtttaccag acaacgagct
    17101 gatgctgcgt gacgtttgcg caccgaaatt acaacaactt ggcgatatcg tcgacgaaat
    17161 gatggctaaa cgccgttaag atggggatac aaaaatgaaa tggtttaaca ctttaagtca
    17221 caaccgttgg ctggaacagg aaaccgatcg catctttgat tttggtaaaa aatccgtagt
    17281 accgaccgga tttggctggt taggcaataa agggcaaatc aaagaagaga tgggcaccca
    17341 tctgtggatc acagctcgta tgttgcacgt ttattccgtt gctgcggcga tgggccgacc
    17401 tggcgcttac tcgttggttg atcacggtat caaagccatg aatggcgcac tgcgcgataa
    17461 aaaatatggc ggttggtatg cctgcgtgaa tgacgagggc gtggtggatg cctccaaaca
    17521 gggctatcaa catttctttg ctctgctggg tgctgccagc gccgtcacta ccggtcatcc
    17581 ggaagcgcgc aagctgctcg attacaccat tgaaattatc gagaaatact tctggagcga
    17641 agaagagcag atgtgcctgg agtcctggga tgaagccttc agcaaaaccg aagagtatcg
    17701 cggcggcaat gccaatatgc acgcggtgga agccttcctg attgtctatg acgtcaccca
    17761 tgacaaaaaa tggctggatc gcgcgattcg cgtggcttcc gtgattatcc acgacgtcgc
    17821 cagaaataat cattatcgcg ttaacgaaca cttcgatacc cagtggaatc cgctgccaga
    17881 ttacaacaaa gataacccgg cgcaccgctt ccgcgcgttc ggaggtactc cgggccactg
    17941 gatcgaatgg ggccgtttaa tgctgcacat tcacgcggcc ctggaagccc gttgcgaaca
    18001 accgccagca tggctgctag aagatgccaa aggtctgttt aacgccaccg tgcgcgatgc
    18061 ctgggcaccc gatggcgcgg acgggattgt ttataccgtt gactgggaag gaaaaccggt
    18121 ggtccgcgaa cgtgtgcgtt ggcctatcgt cgaagcgatg ggtacggcct acgcgctcta
    18181 caccgtcact ggcgatcgcc agtacgaaac ctggtatcag acatggtggg actactgcat
    18241 caaatacctg atggactacg aaaatggttc atggtggcag gagctggatg cggacaataa
    18301 ggtcaccacc aaagtctggg acggcaaaca ggatatttat catctgctgc attgtctggt
    18361 gatcccacgt atcccgttag cgccaggcct ggctccggcg gttgcggcgg gtctgctgga
    18421 tattaacgcg aaataactga ctaaaaaaac gacatatcag gctgcacgct gggtatgtcg
    18481 ttattcaata aaggtacctc gtatgcaaat caccaacatg cattgcagtg gacagaccgt
    18541 ttcacttgcc gcgggggatt atcacgcgac cattgtgacc gtcggtgcag gtctggctga
    18601 attaaccttt cagggctgtc atttagttat tccgcataaa cccgaagaga tgccgctggc
    18661 acatctgggc aaagtcctta ttccgtggcc aaaccggatc gccaacggct gttatcgcta
    18721 tcaggggcag gaatatcagt tacccattaa tgaacatggt tcaaaagccg ctattcatgg
    18781 gctgctcgcc tggcgtggct ggcaaatcag cgaattaagc gccaccagcg tcacattaac
    18841 cgcctttctg ccacccagtt acggctaccc gtttatgctg gcttcgcagg tggtttattc
    18901 gctcaacgcc cgcacgggtc tttccgttga gatcgcaagc cagaatatcg gcactgtcgc
    18961 agcgccttac ggtgtgggta ttcaccctta tttaacctgc aacttaacat ctgttgatga
    19021 atatctgttc cagcttccgg caaaccaagt ttatgccatc gatgagcacg ctaatccgac
    19081 aacgcttcac cacgttgatg agctggattt aaatttcacg caggcgaaaa aaatagccgc
    19141 gacaaaaatc gaccacacct ttaaaaccgc taatgatttg tgggaaatga ctattaccca
    19201 tccacagcag gcgctttcgg tgagcctctg ttccgatcaa ccctgggtgc aggtttatag
    19261 cggtgaaaaa ttacaacgtc agggactggc agttgaacca atgagttgcc cgcctaacgc
    19321 ctttaattca ggtatcgact tattattact ggaaccaggg aaaacgcacc gcctgttttt
    19381 taatattcac gggcagcaca attaatttta ttaagaaaag aacgagatca ttgtttagag
    19441 ttgtatttac acaacaggaa atattatgga cttttttacg ttattttcac cggatggggt
    19501 catgctggaa acaaacgtgg tatgtatcgt tatcgcgtta atgtttcttt atacctttgt
    19561 ggggatatgc gcgggatttg gcggtggttt aaccaccatg ccgctcgtca cgttaatgct
    19621 ccccgtaaaa atggcaactc cgctctcggt gattgtcggc accgcgaccg ccttgtacgc
    19681 cacctggctg tcacgtaaag aaacggattg gcgatcggca atggtactga ttattttctc
    19741 gtttctggga atacccgtcg gtttatatgc gctgtcgtat ttacccgacc attttatgaa
    19801 agtgggctta ggcggattcc ttatcctgta ctccttttac agtatgttta ttctacgcct
    19861 gccgatttat gaccgtagct ggatcgccgc acctatcggc gccgtggcag gagcgttagg
    19921 ggcagcattt tcaaccaacg gaccgccgat ggttatctac ggtatgctgc gtaacttagg
    19981 cccggcggca tttcgcggca ctctgaacgc tttcttcacc gcaaacaaca tcgcggtgat
    20041 cggcgggctg gcgaccagcg gaattttgac tgtctctatc tttaaactgg tgctgttttg
    20101 tattcccacc atgatcctcg gttcactggt tggacaatat gtgcataaac gcatttcggt
    20161 caaagtgttc cgtgtgatgg tctttttatt gctgattgcc tccggttcta tgttgataaa
    20221 aggcgcgctg ggaattcccg ccgctgccgc acttcttcct ccgtttacct tgttagttat
    20281 actgcaactg gcatttggta aaaaagtggc ggcaatccgt cgggctgaag aaagtaaata
    20341 atcattatgc gggttttaat ttatacccgc acacattctt taattaatga aacctgtaat
    20401 aacaggatgg catgaaatat tagttttata tctatggaga ttatttatgg atacgccacg
    20461 cccacagcta atagattttc aatttcatca gaataacgac agttttactc tacgttttca
    20521 agaccgcctt attttaatcc atagcaaaga taatccttgt ttatggattg gttcaggtat
    20581 agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaattgc aggagaaaat
    20641 tgcgcttacc gacgccatcg tcagccagtc accggatggc tggttaatcc atttcagccg
    20701 tggttctgac attagcgcca cgctgaatat ctctgccgac gatcatggcc gtttattgct
    20761 ggaactacaa aacgacaacc ttaaccacaa ccgcatctgg ctgcgccttg ccgcacagcc
    20821 ggaagatcat atctacggct gcggcgaaca attctcctac tttgatctgc gcggcaaacc
    20881 gttcccgctg tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg
    20941 gcaggccgac tgcaaagaga atgcgggagg ggattactac tggaccttct ttccacaacc
    21001 cacgtttgtt agcacgcaga agtattactg ccatgttgat aacagttgct atatgaactt
    21061 cgactttagc gcgccggaat accatgaact ggcgctgtgg gaagacaaag caacactgcg
    21121 ttttgaatgt gctgatacat acatctccct gctggaaaaa ttaaccgcgc tgctgggacg
    21181 ccagccagaa ctgcccgact ggatttatga cggagtaacg ctcggcattc agggcggcac
    21241 tgaggtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg tcaacggcat
    21301 ctgggcgcgg gattggtccg gtattcgtat gacctctttt ggcaaacgcg taatgtggaa
    21361 ctggaagtgg aacagcgaaa actacccgca actggattca cgtatcaagc agtggaacaa
    21421 agagggcgta cagttccttg cctatatcaa cccgtatgtt gccagcgata aagacctctg
    21481 cgaagaagcg gcaaagcgcg gctatctggc aaaagatgcc gccggcggcg attatctggt
    21541 ggagtttggc gagttctacg gcggcgttgt cgatctcact aatccggaag cctacgcctg
    21601 gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga
    21661 cttcggcgag tatctgccca ccgacactta cctgcataac ggcgtcagcg ccgaaattat
    21721 gcataacgcc tggcctgcgc tgtgggcgaa atgtaactac gaagcccttg aagaaacggg
    21781 caagctcggc gagatcctct tctttatgcg cgccggttcc accggtagcc aaaaatattc
    21841 caccatgatg tgggcgggcg accagaacgt tgactggagt ctggacgatg gactggcgtc
    21901 tgtcgtcccg gcggcgctgt cgctggcaat gaccggacat ggcctgcatc acagtgatat
    21961 tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg
    22021 cgatttcagc gccttcacgc cgatgatgcg cacccatgaa ggtaaccgtc ctggcgacaa
    22081 ctggcagttt gacggcgacg cggaaactat cgcccatttt gcccgtatga ccaccgtctt
    22141 cactaccctg aaaccttacc tgaaagaggc cgtcgcgctg aatgcgaagt ccggcctgcc
    22201 ggttatgcgc ccgctgttcc tgcattacga agacgatgca cagacctaca ccctgaaata
    22261 tcagtacctg ttaggccgcg acattctggt cgccccggta cacgaagaag gtcgcagcga
    22321 ctggacgctc tatctgccgg aggataactg ggtccatgcc tggacgggtg aagcgttccg
    22381 gggcggggaa gtgaccgtta atgcgcccat cggtaaaccg ccggtctttt atcgcgccga
    22441 tagcgaatgg gcggcactgt tcgcgtcgtt aaaaagcatc taatcacatc cgcccgtggg
    22501 gaaactcacg ggcaaaggga gaacaacgat gagtcacatc acaacggaag atccagcaac
    22561 tctacgcctg ccctttaaag agaaactctc ttacggtatc ggcgatctgg cctctaacat
    22621 cctactggat atcggcacgc tttatctttt gaagttttat accgatgttc tggggctgcc
    22681 tggcacctat ggcggcatta tctttttgat ttcaaaattc tttaccgcct ttaccgatat
    22741 gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc
    22801 ttttattctg tatgcgtcat ttccggtcac cctgctggcg atcgccaact ttgtcggcac
    22861 accgtttgat gtcaccggta aaacggtgat ggccaccatc ctgtttatgc tttacggact
    22921 gtttttcagc atgatgaact gctcgtatgg cgcgatggtc cccgctatta ctaaaaaccc
    22981 caacgaacgt gcatcgctgg cagcatggcg tcagggtggc gctacattgg gcctgctgct
    23041 gtgtacggtg ggattcgtgc cggttatgaa tcttatcgag ggtaatcagc aacttggcta
    23101 tatcttcgcc gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctattc
    23161 gggcgtgaaa gagcgttatg tcgaaaccca acctaccaat ccggcgcaaa agcctggctt
    23221 gttgcaatct ttccgcgcaa ttgcggggaa ccgcccactg ttcattttgt gcattgccaa
    23281 cctctgtacc ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta
    23341 cgtgcttaac gatcccatcc tgttgtcata tatgggattt ttcagcatgg gctgtatttt
    23401 catcggcgtg ttcctgatgc ctggtgcagt cagacgtttt ggtaagaaga aggtctatat
    23461 cggcggtctg ctgatctggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc
    23521 ggtcagcttt gtggctttct cctgcctggc gttctttggt tcggcgtttg ttaacagcct
    23581 gaactgggcg ctggtttctg acaccgtcga gtatggcgag tggcgcaccg gtgtgcgttc
    23641 ggaaggtacg gtctacaccg gtttcacctt ctttcgcaaa gtatctcagg cgctggcagg
    23701 tttcttccca ggctggatgc tgacgcaaat tggctatgtg ccaaacgtcg cccaggctga
    23761 ccacactatc gaagggttgc gccaactgat cttcatctac ccaagcgcac tggcagtagt
    23821 caccattgtg gcaatgggtt gcttctacag tctgaacgag aagatgtacg tccgcattgt
    23881 tgaagagata gaagcccgta aacgcacggc gtaattataa taaacaacgc cctgcggggc
    23941 gttataagga gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga
    24001 aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta atgcttagca tcggcactct
    24061 gtatctcctc aaattttata ccgatgaatt agggatgcct gcttactatg gcgggattat
    24121 ctttctggtg gcgaagtttt ttaccgcatt taccgatatg ctcaccggat ttttactcga
    24181 ctcgcggaaa aatattggac ctaaaggaaa attcaggccg tttattttat acgcagcgat
    24241 tccggcggcg ttaattgcaa cgctccagtt tatcgccacc acctttagtt tgccggttaa
    24301 aacgacgatt gccaccgcac tgtttatgat gtttggactt tcatacagtc tgatgaactg
    24361 ctcgtatggc gcgatgatcc cggccattac caaaaacccg aatgagcgcg cgcaactcgc
    24421 tgcttaccgc cagggtggtg caaccatagg gctattgatc tgtaccgtgg cgtttattcc
    24481 tttgcagtca ctattttctg actccaccgt cggctacgcc tgcgcggcac tgatgttctc
    24541 cattggcggc tttattttta tgatgctgtg ctacagaggt gtcaaagagc attatgtgga
    24601 cacgacgcca accggacata aggccagtat tctcaaatca ttttgcgcga tatttcgtaa
    24661 tccacctttg ctggtattgt gcattgctaa cctgtgtacc ctggcggcat ttaatatcaa
    24721 actggcgatt caggtctatt acacccagta tgtgctgaat gatattaact tattgtcgtg
    24781 gatgggattc tttagtatgg gatgcattct cgtcggcgta ttactggtgc cactaactgt
    24841 aaaatgtttt ggtaaaaaac aggtttatct ggctggcatg gtgctgtggg cggtggggga
    24901 tatcctgaat tattgctggg gcagtaactc attcactttc gtcatgttct cttgtgtcgc
    24961 cttttttggc acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga
    25021 ttatggtgaa tggaaaacag gtatacgtgc cgaaggttct gtttataccg gttatacctt
    25081 ctttcgtaaa atttcagccg cacttgctgg cttcttgccc ggcattatgc tgacgcaaat
    25141 cggttatatt cccaacatcg cgcaaagcga tgcaacatta cagggtttgc gtcagctcat
    25201 ttttatctgg ccttgtgcgc tggcaattat tgctgcattg acgatggggt tcttttacac
    25261 gctcaatgag aaacggtttg cactgattat tgaggaaatt aaccaacgca aaaataaaga
    25321 aattgagaca gaagaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc
    25381 catcctgatg ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaatatta
    25441 atgcaataat tttattatct tctctgacct ctgcctcggt attcgctggc gcatacgtcg
    25501 aaaacaggga agcatataat ctcgcctccg atcagggtga ggttatgcta cgtatgggtt
    25561 ataacttcga tatgggcgcg ggtattatgc taaccaatac ctacaccttt cagcgagaag
    25621 atgaactcaa acatggctat aatgaaattg aaggttggta tcctttattt aaacctaccg
    25681 ataaattaac catccagccc ggtggcttaa ttaatgacaa gagtatcggt tcgggtggtg
    25741 ctgtttatct ggatgttaac tacaaattta ctccgtggtt taacctgacg gtacgtaatc
    25801 gctataacca taataattac agctctacag atttgaacgg cgaattggat aataacgaca
    25861 cctatgaagt tggcacctac tggaacttta aaataacaga aaaattttcc tatacatttg
    25921 aaccacatta cttcatgcga gtgaatgact ttaatagtag caacgggaaa gatcatcatt
    25981 gggaaatcac taacaccttc cgttaccgta ttaatgaaca ctggcttcct tatttcgaat
    26041 tgcgctggtt agaccgcaac gtcgagccgt accaccgcga gcaaaaccag attcgtatcg
    26101 gaacgaagta tttcttctga tgtaaaaatt cctgatgcga tgctggcgca tcttatcagg
    26161 tctacaaaag ccaaaaaaaa cctgccatcg ctggcaggtt ttttatgact gaaaaacgaa
    26221 attaatcgtc tttcggtgcg cggttggcgc ggcgacgatc attttccgtc aggtgacgtt
    26281 tacgaatacg aatagaggtc ggagtcactt ctaccagttc gtcatcatcg atgaactcca
    26341 gagcttgttc cagagtcatg cggataggcg gaaccagaac aacggcttcg tcagtaccgg
    26401 aagcacgcat gttggtcagt ttcttaccgg tcaggcagtt tacagtcagg tcgttagagc
    26461 ggctgtgaat accgataatc tgaccttcgt aaacttctgc accgtgaccg aggaacagct
    26521 taccgcgatc ctgcagaccg aacagcgcga acgctaccgc tttaccctga ccgttagaga
    26581 tcagtacgcc gttctgacgc tggcccactt cacccggacg tacgtcgtcg tagtggctga
    26641 aggtggagta cagcagacca gtaccggaag tcatggtcat gaactcagaa cggaagccaa
    26701 tcagaccacg gcttgggatc acgtagtcga gacgtacgcg gcctttaccg tctggattca
    26761 tgtttttcag gtcgccttta cgttcgccca gcgcctgcat tacagaaccc tgatgctgtt
    26821 cttcaacgtc cagcgtcacg ttttcatacg gctcttgttt acgaccgtcg atttcacgga
    26881 agataacctt cggacgggat accgccagtt cgaaaccttc acgacgcatg ttttcgatca
    26941 gaacagacag gtgcagttcg ccacgaccag aaacgcggaa cgcatcggcg tcttcggttt
    27001 cttctacgcg cagcgcaacg ttgtgtacca gttctttgtt cagacgatcc aggatctgac
    27061 gagacgttac gaacttacct tctttaccgc agaacggcga ggtgttaacg cagaagaaca
    27121 tagaaacggt cggctcatca acggagagtg ccggcagcgc ttcaacgttt tgcgtgtcgc
    27181 aaacggtgtc agaaatgttc agttcgccca ggcccgtgat cgccacgata tcgccagctt
    27241 ccgccagatc ggtttcgata cgttccagac cgaggtggcc cagcacttta ccgactttcg
    27301 cgttgcgggt tttgccttcg ctatcgatga tagtaacctg ctggttcggc ttcactttac
    27361 cgcgcttgat gcggccaatg ccgataacgc caacatagct gttgtaatcg agctgagaaa
    27421 tctgcatctg gaacggaccg tcaaggtcaa cgtccggcgc aggaacgtgg tcaacaatcg
    27481 cctggtacag cggggtcatg tcttccgcca tatcttcgtg gtccagaccc gcgataccgt
    27541 tcagcgcaga agcgtaaacg atcgggaagt ccagctgctc gtcggtcgcg tcgaggttaa
    27601 cgaacagatc gaatacctga tccacaaccc aatcaggacg cgcgccaggg cggtcaactt
    27661 tgttgataac aacaatcggc ttcaggccgt aagcaaacgc ttttttggtt acgaagcgcg
    27721 tttgcggcat cgggccgtca aatgcgtcaa ccaccagcag cactgagtct accatggaca
    27781 ttacacgttc aacttcacca ccgaagtcgg cgtgccccgg ggtatcaacg atgttgatac
    27841 ggtaatcatt ccatttgata gcggtgtttt tcgcgaggat ggtaatccca cgctctttct
    27901 ccaaatcgtt ggagtccatc acgcgctctt gggtttcggc acgagagtcg aacgtaccgg
    27961 attgttggag cagcttgtct accagggtgg ttttaccatg gtctacgtgc gcgatgatgg
    28021 cgatattacg caatttttcg atcacaactt tgcctcaggc attagaaata gcgcgttatt
    28081 gtacacggat taatcgcact acaaaacagg atcacaaaca tcctccgcaa acaagtattg
    28141 cagagtccct ttgtgatcgc tttcacggag cataaaaagg gttatccaaa ggtcattgca
    28201 ccaatatggt gcttaatgtt cccattgaag cactatattg gtgcaacatt cacatcatgg
    28261 tgcagccctt ttgcacgatg ttaagcatga taacgccttt taggggcaat ttaaaagttg
    28321 gcacagattt cgctttatct tttttacggc gacacggccc atttaattgc agatttcgtt
    28381 accacgacga caatgaccaa tccaggagag ttaaagtatg tccgctgaac acgtactgac
    28441 gatgctgaac gagcacgaag tgaagtttgt tgatttgcgc ttcaccgata ccaaaggtaa
    28501 agaacagcac gtcactatcc ctgctcatca ggtgaatgcc gaattcttcg aagaaggcaa
    28561 aatgtttgac ggctcctcga ttggcggctg gaaaggcatt aacgaatccg atatggtgct
    28621 gatgccagac gcatccaccg cagtgattga tccgttcttc gccgattcta ccctgattat
    28681 ccgttgcgac atccttgaac ctggcaccct gcaaggctat gaccgtgacc cgcgctctat
    28741 tgcgaagcgc gctgaagatt acctgcgttc tactggcatt gccgacaccg tactgttcgg
    28801 gccagaacct gaattcttcc tgttcgatga catccgtttc ggatcgtcta tctccggttc
    28861 ccacgttgct atcgacgata tcgaaggtgc atggaactcc tccacccaat atgaaggtgg
    28921 taacaaaggt caccgtccgg cagtgaaagg cggttacttc ccggttccgc cggtagactc
    28981 agctcaggat attcgttctg aaatgtgtct ggtgatggaa cagatgggtc tggtagttga
    29041 agctcatcac cacgaagtcg ctaccgcagg tcagaacgaa gtggctaccc gcttcaatac
    29101 catgaccaaa aaagctgacg aaattcagat ctacaaatat gtcgtgcaca acgtggcgca
    29161 ccgtttcggt aagaccgcga cctttatgcc aaaaccgatg ttcggtgata acggttccgg
    29221 tatgcactgc cacatgtcgc tgtctaaaaa cggcgtcaac ctgtttgctg gcgacaaata
    29281 cgcaggtctg tctgaacagg cactgtacta cattggcggc gtaatcaaac acgctaaagc
    29341 gattaacgcc ctggcaaacc cgaccaccaa ctcttacaag cgtctggtcc cgggctacga
    29401 agcaccggta atgctggctt actctgcgcg taaccgttct gcgtctatcc gtattccggt
    29461 ggtttcttct ccgaaagcac gtcgtatcga agtacgtttc ccggacccgg cggctaaccc
    29521 gtacctgtgc tttgctgccc tgctgatggc cggtcttgat ggcatcaaga acaagatcca
    29581 tccgggcgaa gccatggaca aaaacctgta tgacctgccg ccagaagaag cgaaagagat
    29641 cccacaggtt gcaggctctc tggaagaagc actgaacgaa ctggatctgg atcgcgagtt
    29701 cctgaaagcc ggtggcgtgt tcacggatga agcgatcgat gcgtacatcg ctctgcgtcg
    29761 cgaagaagat gaccgcgtgc gtatgactcc gcatccggta gagtttgagc tgtactacag
    29821 cgtctaatag ttgaagttgt actacccggc gcaacaacgc cgggatttag ttgccgtgga
    29881 aactttcagc ccatctctgc atgggctttt ttctccgtca attctctgat gcttcgcgct
    29941 ttttatccgt aaaaagctat aatgcactaa aatggtgcaa cctgttcagg agactgcttt
    30001 atggcaacag gcacgcagcc cgatgctggg cagatcctca actcgctgat taacagtatt
    30061 ttgttaatcg atgacaacct ggcgatccat tacgccaacc ccgccgcgca acaactgctc
    30121 gcccaaagct cccgcaaatt gtttggtaca ccgttaccgg aactgttgag ctacttctca
    30181 ttaaatatcg agctgatgca agaaagtctg gaggcggggc aaggttttac cgataacgaa
    30241 gtgacgctgg tcatcgacgg gcgctcgcat atcctttctg tgacggctca gcgtatgccg
    30301 gacggcatga tcctgctgga gatggcaccg atggataacc agcgccgctt aagtcaggaa
    30361 cagctacagc acgcccagca ggttgctgcc cgtgatttag tgcgcggcct ggcacatgag
    30421 attaaaaatc cgcttggcgg tttacgtggc gcggcgcagc tactcagcaa agcattacct
    30481 gacccgtcac tactcgaata taccaaagtg attatcgaac aggcggaccg gctgcgaaat
    30541 ctggtcgacc gtctgttggg gccgcagcta cccggtacgc gcattaccga aagtattcac
    30601 aaagtggctg aacgcgtggt gacgctggtg tcgatggaac tgccgaacaa cgtgcggttg
    30661 attcgggatt acgaccccag cctgccggaa ctggcgcacg acccggatca aattgaacag
    30721 gtcttgctga atattgtgcg caatgcgcta caggcgctgg ggccggaggg tggtgaaatc
    30781 attctgcgta cccgcacagc gtttcaactg accttacacg gcgagcgcta tcggctggcg
    30841 gcgcggattg atgtggaaga taacgggccg ggcattccgc ctcatttgca ggatacgctg
    30901 ttttacccga tggtcagcgg ccgcgaaggt ggcaccgggc ttggcttatc catcgcccgt
    30961 aatttgattg atcagcattc aggcaaaatt gaatttacca gttggccagg tcataccgag
    31021 ttctcggttt acctgcctat caggaaataa aggtgacgtt tatgcaacga gggatagtct
    31081 gggtagtcga tgacgatagt tccatccgtt gggtgcttga acgtgcgctc gctggagctg
    31141 gtttaacctg tacgacattt gagaacggcg cggaagtact ggaggcgctg gcgagcaaaa
    31201 cgccggatgt gctgctttca gatatccgta tgccgggaat ggacgggctg gcgctgctca
    31261 agcagattaa acagcgccat ccgatgcttc cggtcatcat tatgaccgca cattccgatc
    31321 tggatgctgc cgtcagcgcc tatcaacaag gggcatttga ttatctgccc aaaccgtttg
    31381 atatcgacga agccgtggcg ctggttgagc gcgccatcag tcattaccag gaacagcagc
    31441 agccgcgtaa tgttcagctt aacggcccaa cgaccgatat catcggtgaa gcgccagcca
    31501 tgcaggacgt gttccggatt atcggtcggc tttcgcgttc ttctattagc gtgctgatta
    31561 acggcgaatc cggcaccggt aaagaactgg tcgctcatgc cctgcatcgc cacagtccgc
    31621 gagccaaagc gccgtttatc gcgctcaata tggcggctat cccgaaggat ttgatcgaat
    31681 cagaactgtt tggtcacgag aaaggcgcat ttaccggcgc gaataccatt cgtcaggggc
    31741 gttttgaaca ggctgatggc ggtacattat ttctcgatga aattggcgat atgccgctgg
    31801 atgtgcagac gcgtttgctg cgcgtgctgg cagacggtca gttttatcgc gttggcggct
    31861 atgcgccggt gaaagtagat gtgcggatta tcgctgccac tcaccagaat cttgaacagc
    31921 gggtgcagga aggcaagttc cgtgaggatc tgttccaccg cctgaacgtc atccgcgttc
    31981 atctgccgcc actgcgcgaa cgtcgggaag atattccccg tctggcacgc cattttttac
    32041 aggttgccgc gcgagagctg ggcgtagaag cgaagttgct gcatccggaa accgaagccg
    32101 cgctgacgcg tctggcgtgg ccaggcaacg tgcgccagct ggaaaacacc tgtcgctggc
    32161 taacggtgat ggccgccggg caggaagtgt tgattcagga tttgcctggc gaactgtttg
    32221 aatcaaacgt accggaaagc acttcacaca tgcagccgga cagttgggca acgctgttag
    32281 cacagtgggc agacagagcg ctgcgttccg gtcatcaaaa cctgctttcc gaagcacagc
    32341 cagagctgga acggacgtta ctggccaccg cgctgcgaca tacgcagggg cataaacagg
    32401 aagcggcgcg gctacttggc tggggccgca acaccctgac gcgtaagtta aaagagctgg
    32461 ggatggagtg attcacggct tgtgtgtaaa gattgattat tgagcgcaaa ttgctggtat
    32521 tttgcgcttt actgttccga taagttcagt atgatcttgc ccggaaaacg gggagagtca
    32581 ttatgctgga atcaataatt aatctggtat cgagtggcgc agttgacagc cacacaccgc
    32641 aaactgctgt tgctgccgtg ctgtgtgccg cgatgatagg gctgtttagc tgaagctcgt
    32701 tgtttacgcc ggatgcggag aaagtcgtag gcctgataag acgcaaccag cgtcgcatca
    32761 ggcgttgatt gccggatgcg gcgtaaacgc cttatccggc ctacaaaagc aataatttca
    32821 acagattttg caggcctgat aagcgtagcg catcaggcaa tcttgttctc agcacattga
    32881 tggcgctttc gttttctact ttgtaaacga agcgccattc acaatttaaa tcacccgaga
    32941 gaactgctgc atccgcgctt tctggcgcag ataggtatca aagcacatgc aaatgttgcg
    33001 gatcagcaag cgacctttcg ccgtcacctg tattcccttc tcatccacat ccaccagccc
    33061 atcttttgct aacggggcga gcagcttgag atcttccgca aagtaatcag cgaagagcaa
    33121 atcccactgt tgctcaatag gggcgtaatc cagacggaag ttgcagatga gcgacttaat
    33181 cacatcgcgg cgaatacagt catcacgcgt tagcgcaata ccacgccaca gcgcattgcc
    33241 ttgttcatcc acttgctgat agtactgctt caactctttc tggttctgcg cgtagcagtc
    33301 gccaatcatg ctgatggcgg aaacgcccat cccaagcaga tcggtatcgc cctgggtggt
    33361 gtagccctgg aagttacgat gcagcacgcc ttcacgctgg gccaccgcca gctcgtcatc
    33421 cgggcgagca aagtgatcca taccgataaa ctgatagccc gattgtgtca ggaaggcgat
    33481 ggtttcctgc agaatatcga gtttttgttg cggactcggc aagtcagcat ctttgatttt
    33541 gcgctgggcg gcaaaaatag ttggcaaatg cgcgtagtta aagacgctca gacgatcggg
    33601 gttcagctcc gccacgcgtt tcagggtaaa ggcgaaactc tccggcgtct gtttcggcag
    33661 gccgtaaatc aggtcgatgt tggtggaggt aaagccaatc tcacgcgcat ggttaagcag
    33721 tgcaaagatg aactcttcat cctgctcgcg gttaaccaga cgttgcactt ctttgttgaa
    33781 gtcctgcacg cccatgctca gccgattaaa gccttcggcg cgtaaatgat cgagtacatc
    33841 cagttcgatt tcccgcggat cgacttcgat cgagatctcc gcatcggcat tgaactggaa
    33901 gttttcgcgc agcagcttca tcagacggct gatttgcgct ttattcagat acgtcggcgt
    33961 tccgccgccc cagtgcaatt ggctgacgtg acgcccggcg aacagcggtg cacgatggac
    34021 gatttcttgc tccagcgcgt ccagatactg atcggccttg tgctgctggc gagtaacaat
    34081 cttattgcaa ccgcagaagt aacaaagctt atggcagaac ggaatatgta cgtagagaga
    34141 taatggacgc tcaggatagc gcgccacggc ttgtaaaaac gcctgttcgc cgaagtcttc
    34201 tgaaaactcc agcgcggtcg ggtacgaggt gtatcgtggc ccggaatagt tatatttctg
    34261 gatcagggcc agatcccagt cgatttgctg tacagacatg ctcactcctt ccgatggcgt
    34321 ctctggcggc tacggcgtac cggcggatgc ccgttgcgag tcatcagccg gttgcgcagc
    34381 cacttttgtc gccgcgacaa ccgctgtagt ttaacgaata accacaccag ataacatata
    34441 accggaaggg ttatcagcag aacgggaagg cccacggcgg caatccgtta gttgccccgc
    34501 agcagacgca tcatgtcttc ttgcttctcg tcttcttcct cttcttcgtc atcgtcataa
    34561 gagaggccga gtttctgcat caactcatca atacgatcca gtttggcatc cacccaggat
    34621 tgctcttcgg cgctcagggt ttcgcctgct tccagacgtt ccagcagcgc atccagacgc
    34681 tcatccgttt ccagtaactc caactccgcc tgcggtgaaa gcataggttt ctcactcttc
    34741 ggtttgtgct gtttggtgac tttttcagtc acgcccaatg gaataggggt tttactgcca
    34801 atacgtggat cttttggtgc gttctgacct ttgctgcctg acgtggtgtt accgcccgct
    34861 gcgcggctgc ccggcgcatg accacgacgt tttttctgac gcttacggtc acgagcttcc
    34921 tgatccagct cctcgcgtgt ttttcgacgc gcttttgcat gacctttgct gcgtgagttt
    34981 gaagatgatg gtttcataat ggttcgtctt ttagccgttt tattcagtat agatttgcgg
    35041 cggaatctaa cagaaagcaa gcaaagaaaa aaggcgacag attactctgt cgcctttttt
    35101 cctgactcat aacccttaac gggtcttaca atccttgcag gccgccaaca caccctgttt
    35161 ttccattagc cggaacaatc cgtgttgatt ccatttccat ttaattacgt ctccggacgt
    35221 ttatcttcct gacaatcctc tgtcttcgcc tcctggcgct cctgaccctc atcctgagtc
    35281 attgttcctg ttagcgtcct cgctctatgg gtgctacttt acgcctttgc tttaaataaa
    35341 caatcaaccg ccatcagtat ttgcacattt tcagagtttt atataaaagc aattcactga
    35401 tttatatagc ttatttgaca tacgtcgcag aattttctct gctatttcgc ataaagacta
    35461 tcggcaatgt ctcacaaagc gtatggcttt tacttacaac gctcacgccg aaaaatccgc
    35521 cttttattat ggttcaggta taatcgccgc aaagctttga ctgatggaga cgaccgcttt
    35581 gactaatttg aattatcaac agacgcattt tgtgatgagt gcgcctgata ttcgccacct
    35641 accttccgat accggaattg aagtggcttt tgcaggccgt tccaacgcag gtaaatccag
    35701 cgcgctgaac acgctgacta accagaaaag cctggctcgt acctcaaaaa ccccggggcg
    35761 cacccagctt atcaacctgt ttgaagtggc tgacggcaag cgtctggttg acttgcctgg
    35821 gtacggttat gcggaagtcc cggaagagat gaagcgcaaa tggcagcgcg cgctcggcga
    35881 gtacctcgaa aaacgtcaga gcctgcaagg tctggtggtg ctaatggata ttcgccatcc
    35941 gctgaaagat ttggatcagc agatgattga gtgggcggta gacagcaata tcgccgtgct
    36001 ggtactgctg accaaagcgg acaaactggc aagcggcgca cgtaaagcgc aattgaatat
    36061 ggtgcgtgaa gcggtgctgg cgtttaacgg tgatgtgcag gttgaaacgt tttcttcgct
    36121 gaagaaacaa ggcgtggaca agctgcggca gaaactggat acctggttta gcgagatgca
    36181 gcctgtagaa gaaacgcagg acggcgaata attttcttgc cttaatgctt gtgccggatg
    36241 tggcgtatcc ggcccgtaaa ttcaatagat tacaattcta ccgttggcct gataaacatg
    36301 gcgtatcagg cattacggat cttttctttc gcccaataaa aaacgcccca gtcattactg
    36361 actggggcgg ctaaaatatt cagccaaatc cgattacgtg aagtaaaagg tctgaaagat
    36421 agaacatctt acctctgtac cctacgcgac taactttact cttttttgtt cagttcagaa
    36481 agcatttttt gtaatttttt ttcagttgtt gcataggaat ttctaatagc catcacaaaa
    36541 cgtgacagct tatgttgctt acttacgaaa aaagggcatg ttcaggcgaa tcttagtgcg
    36601 cctgatccca gttttcgcca ctccccactt ccaccagtaa cggcacatcc agacgggtac
    36661 agttttccat cagttgatga atctgcttcg cgacggcatc gacatcatct ttatggactt
    36721 caaataccag ttcatcgtgt acctgcatga tcatacgcac gcgcggttgc tcagcctgta
    36781 gccacgcatc aacggcaatc atcgcccgtt tgataatgtc ggcggcggtt ccctgcattg
    36841 gcgcgttaat ggctgcacgt tcagccgctg cacgacgagc accattgctg gatttgatat
    36901 ccggcagata cagacggcgt ccgtccagcg tttcaacgta gccctgctct ttcgcctgag
    36961 cacgggtgcg ttccatatac tccagcacgc cagggtagcg ttcgaagtaa aggtccatgt
    37021 acttctgcgc ttctttacgc ggaatgttca attgccgcgc cagaccgaaa gcactcatgc
    37081 cataaatcag accaaagttg atcgcttttg cactacgacg ttgctcgctg gtgacggttt
    37141 ccagtggcaa accaaacact tctgccgccg ttgcacggtg gatatctttt ccttccgcga
    37201 atgcggtcag caagcctttg tcacgcgaga gatgcgccat aatgcgcagt tcaatctgcg
    37261 agtagtccgc tgagacaatc acataatcct ctggcgcaat aaacgcctgg cggatacggc
    37321 gaccttcttc gttacgcacc ggaatgtttt gcaggttagg atcggttgac gataaacgtc
    37381 ccgttgcggt tactgcctgg tgataagagg tatgtacacg cccggttttc gggttgatca
    37441 tcaacggcag cttgtcggtg taggtcgatt tcagcttcgc cagaccacga tactccagaa
    37501 tcacttttgg caacggatag tccagcgcca gttcttccag tacctcttcc gacgttgatg
    37561 gcgcgccacc cggcgttttc ttcagtggtt taatgccctg tttttcaaac agaatggttt
    37621 gtaactgcct ggtggaagaa aggttaaatt cttcacctgc aatttcatga gctttctttt
    37681 ccagctcagc cagacgcaag gtgagctctt cagagtgatt gtgcagcact ttcggatcga
    37741 tcttcacacc gttacgttca atgcgtgaaa gcaccggcac cagcggcatt tcgatattct
    37801 cgaagacgtt caacggccct ttgtgttttt gcagatccgg ccacattttc agatgcaact
    37861 gcaaggtgac atctgcatct tcggcggcgt aacgtccggc ttcttcgagg gcaatctggt
    37921 taaaggtcag ttgatttttg cctttacccg caatctcttc aaaagtgatg gttttgtgct
    37981 tcaaccaacg ttccgcgagg ctgtccatat cgtgacgtcc ggcaacgcta ttgagaatgt
    38041 aggactccag catggtatca aacgcaatcc cacgtagttc aatgccgtag ttcgccagaa
    38101 taccgcgatc gtatttcagg ttttgcccga ccttcagcgc cttttcatct tccagcagcg
    38161 gttttagcaa ctctagtgca cgctcgcgag agatttgatc gggcgcatca agataatcat
    38221 gagcaaccgg aatatatgcc gctacgcctg gctcgatagc aaaagaaagc ccgaccaggt
    38281 tagcagagat gttatcaagg ctgtcggttt cggtatcaaa tgcaaatacc ggcgcttttt
    38341 ccagcttcgc aatccactct ttcagtgttt cttcatcaag gatggtgacg tagttgtcat
    38401 aagaaatcac cgttgccgtc acttctggcg cttcgtctgc aacactggtt tcctgcggcc
    38461 tggctgcggg tttcgcccct ttagcctgta gccattttcc cgcttcgaca tcagcagtcc
    38521 agcgtttgaa ctcatacttt ttgaacagac ctaacaactc ttccgccgcc ggtggctgca
    38581 cttccagttg ttcacaggtc agctccagtt caacgtcggt tttaatcgtc gccagctggt
    38641 atgagagata agcaacttct ttgttttgct cgagcttcgc tgccattgtt ttcgcgccac
    38701 ggaagctcaa cccagcaatt ttttctggct cggcatacag cgtatccagc ccgccaagac
    38761 cttgcagcaa tgcctgcgcg gttttttcac cgacgcccgg tacgccagga atgttatcag
    38821 aggagtcacc catcagcgcc aggaaatcga tgatcagttc tggcggcacg ccgtacttat
    38881 tcaccacctc ttccggtccg aggatggtat tcgtcatggt gttgataagc gtgatatttg
    38941 gtgtcaccag ctgcgccata tctttatcgc cagtgctgat cagcaccgga cgcccggctt
    39001 tttcggcttc gcgcgccaga gtaccaataa cgtcgtccgc ttctacgcca gaaaccgcca
    39061 gcagcggcag tcccatcgct ttaaccatcg cgtgcagagg ttcgatttgt gctcgcagat
    39121 cgtccggcat tggcgggcga tgtgatttgt aatgttcaaa cagttcatca cgaaaggttt
    39181 ttcccttggc gtcaaagacc accgctgcat gcgtcggttt atattgcatg atcagactgc
    39241 gcagcatgtt gaggacacca tacatcgcac cggtcggctc gcctgcgctg ttagtcagcg
    39301 ggggaaacgc gtgatatgcg cgataaaggt aagatgaacc atctacaagg ataagtggat
    39361 tttgggggat ctgaaccata atgtccgtgc ctgtttatca gattatgggt aaaggatgcc
    39421 acagacagga tgaaaacatg agtttttcac cgcttttcgt gaaaatcttt tgtagatctt
    39481 ctggatcgct cgcaaatcgt catgtggata actttgtgca taaattattt acatcgcttt
    39541 taatttatta tcatgctttt tacagcatga tgtttgtctt tgtttttcat gcagttaaaa
    39601 ataaagccat ccctaataat tgagataatg ggattttttc taatgtggat agatatgaat
    39661 tatttttctc cttaaggatc atccgttatt tgggtcgttt tctgctaaaa tctgcgcctt
    39721 gcgcttcttt agcgcactgt ctatggctaa ccttttgaat aaattcatta tgacgagaat
    39781 actcgctgcg ataacccttt tgctgagtat cgtattgacc attttggtca ctattttttg
    39841 ttccgtcccg atcatcattg ccgggattgt aaaactattg ctacctgtgc cagtcatttg
    39901 gcgaaaggtt tcgcgttttt gtgattttat gatgtattgc tggtgcgaag gtctggcggt
    39961 attactgcat ctgaacccac acctgcaatg ggaagttcac gggctggaag ggttaagtaa
    40021 gaagaactgg tatctgctta tttgtaacca ccgtagttgg gcagatattg tcgtactgtg
    40081 cgtgctgttt cgtaagcaca ttccgatgaa taaatatttc ctcaagcagc agctggcctg
    40141 ggtgcctttt cttggcctgg cgtgctgggc gctggatatg ccgtttatga agcgttattc
    40201 ccgcgcttat ttattacgtc atccggagcg acgtggtaaa gatgttgaaa ccactcggcg
    40261 ttcttgtgaa aagtttcgcc tgcatcccac cactattgtc aatttcgttg aaggctcccg
    40321 tttcacgcaa gaaaaacatc agcaaaccca ctccactttt caaaacttgt tgccaccaaa
    40381 ggcggcaggc attgcaatgg cgctaaatgt actgggtaaa caattcgata aactgcttaa
    40441 tgttacgctg tgttatccgg acaataaccg tcagccgttc ttcgatatgt taagc
//
LOCUS       DAZGJT010000033        39876 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZGJT010000033 DAZGJT010000000
VERSION     DAZGJT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39876
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.033"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..418
                     /gene="yiaM"
                     /locus_tag="ACHKFK_001922"
     CDS             <1..418
                     /gene="yiaM"
                     /locus_tag="ACHKFK_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO9052271.1"
                     /translation="CIIFINIILRYGFHTSILSIDELSRLLFVWLTFIGAIVAYMDNS
                     HVQVTFLVEKLSPANQQRVSLLTHTLILLLCLGLAWGAIEKTAQDWSNLSPILGVPIG
                     LMYAAAIPTSLIIALLELRHLYRQFTNTPSRNQQGA"
     gene            421..1698
                     /gene="yiaN"
                     /locus_tag="ACHKFK_001923"
     CDS             421..1698
                     /gene="yiaN"
                     /locus_tag="ACHKFK_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO9052272.1"
                     /translation="MTILIFIVSLLGAIAIGVPIAWALLLCGISLMFWMDIFDVQILA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSQRIVDLPMKLVGHRPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRQANYPVNRAAGLIGSGGIIAAIIPPSIPLIIFG
                     VSSGLSISKLFMAGIAPGIMMGVTLMVTWWWQAKRLNLPCQPKASLREVWQSLVSGIW
                     ALFLPIIIIGGFRSGLFTPTEAGAVAAFYALFVSVVVYREMTFSTLYHVLINAAKTTS
                     VVMFLVASAAVSAWLITIAELPMMVSELLQPLVDSPRLLFIVIMLAIMVISTVMDLTP
                     TVLILTPVLMPLVKEAGIDPVYFGIMFIINCSISLITPPVGNVLNVVCGVAKLKFDDA
                     VKGVAPYVMVLFMLLALFIFIPELITAPLKWMS"
     gene            1711..2697
                     /gene="yiaO"
                     /locus_tag="ACHKFK_001924"
     CDS             1711..2697
                     /gene="yiaO"
                     /locus_tag="ACHKFK_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO9052273.1"
                     /translation="MKLRSVTYALLIAGVATFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFTGLSPVIN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKVSLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FYEVQKYLSLTHHAYSPLLVVINKAKFDGLTPEFQQALISSAQEAGSYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDTLGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            2701..4197
                     /gene="lyxK"
                     /locus_tag="ACHKFK_001925"
     CDS             2701..4197
                     /gene="lyxK"
                     /locus_tag="ACHKFK_001925"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO9052274.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGRA
                     ERDMAELWQCCTAVIRALLTHSGVSGEQIVGIGISAQGKGLFLLDKNDKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSRGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGLEDEFTLNAVMGTWAVTSGITHGL
                     RDGEAHPYVYGRYVNDGQFIVHEASPTSSGNLEWFTAQWGEISFAEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPNMAAHQLYQQKYQRYQHLIAALQGYHARIKEHTL"
     gene            4194..4856
                     /gene="ulaD"
                     /locus_tag="ACHKFK_001926"
     CDS             4194..4856
                     /gene="ulaD"
                     /locus_tag="ACHKFK_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9052275.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTLLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QNCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKEIRVKAFIAGRALAGAANPAQVAADFHAQIDSIWGGK
                     RA"
     gene            4849..5709
                     /gene="sgbU"
                     /locus_tag="ACHKFK_001927"
     CDS             4849..5709
                     /gene="sgbU"
                     /locus_tag="ACHKFK_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO9052276.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSAAQRASLVTAMIETGVAIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTQQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     HSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            5703..6398
                     /gene="araD"
                     /locus_tag="ACHKFK_001928"
     CDS             5703..6398
                     /gene="araD"
                     /locus_tag="ACHKFK_001928"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO9052277.1"
                     /translation="MLEQLKADVLAANLALPVHHLVTFTWGNVSAIDETRQWMVIKPS
                     GVEYEVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRFAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRLMTAEEINGEYEYQTGEVIIKTFEERGLNP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(6445..7983)
                     /gene="aldB"
                     /locus_tag="ACHKFK_001929"
     CDS             complement(6445..7983)
                     /gene="aldB"
                     /locus_tag="ACHKFK_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO9052278.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTIEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(8091..9386)
                     /locus_tag="ACHKFK_001930"
     CDS             complement(8091..9386)
                     /locus_tag="ACHKFK_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFO9052279.1"
                     /translation="MAIKSPPGLIPLSHLSGEELLAHLRFNRVTDEKGRYLPFDELQY
                     RIKKGENVDVAWTLTRLARNAAIQRINYCNEAGEQAGFNITPVIAEACELVDKRATAL
                     ALKDQTERLRGAGAELSQLRLEEPITSSQLEGANTTTLVARKMLETGRSPRTEDEHMI
                     AGNARLMTEIPHLLAEPLTPALIRQLHAIGMGGINDAKYRPGEFRETDDVVIADYDGN
                     IVHQPPAAALLPERLEKVCQWLNSHEGYIHPLVRACILHFMLAHEHPFRDGNGRTSRA
                     LFYWYMLKSGYDVFKYISISRLLHAAPVKYAASYQYTESDGMDLTYFLEYQAGVIKRA
                     LQNWQQHIDEITQRSAKLDSVLFSSGVLKRLNPRQVTLLNVMLANPGKEYTVAEISAS
                     LGVSDNTARADLRTIVKEGFAQEKKINDQQAVYFAHYPL"
     gene            complement(9516..10667)
                     /gene="yiaY"
                     /locus_tag="ACHKFK_001931"
     CDS             complement(9516..10667)
                     /gene="yiaY"
                     /locus_tag="ACHKFK_001931"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO9052280.1"
                     /translation="MAASTFFIPSVNVIGADSLNDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            complement(10858..12702)
                     /gene="selB"
                     /locus_tag="ACHKFK_001932"
     CDS             complement(10858..12702)
                     /gene="selB"
                     /locus_tag="ACHKFK_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO9052281.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEGKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHTLLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADKDRLVLRDISARNTLAG
                     ARVLMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDIHSHHGWLHLPDHKAGFSAEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(12699..14090)
                     /gene="selA"
                     /locus_tag="ACHKFK_001933"
     CDS             complement(12699..14090)
                     /gene="selA"
                     /locus_tag="ACHKFK_001933"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO9052282.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSTFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVTKAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(14188..14796)
                     /gene="yibF"
                     /locus_tag="ACHKFK_001934"
     CDS             complement(14188..14796)
                     /gene="yibF"
                     /locus_tag="ACHKFK_001934"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO9052283.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(14867..16003)
                     /gene="yibH"
                     /locus_tag="ACHKFK_001935"
     CDS             complement(14867..16003)
                     /gene="yibH"
                     /locus_tag="ACHKFK_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9052284.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPDA
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(16006..16368)
                     /gene="yibI"
                     /locus_tag="ACHKFK_001936"
     CDS             complement(16006..16368)
                     /gene="yibI"
                     /locus_tag="ACHKFK_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO9052285.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVAPQGDVPDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            16905..18818
                     /gene="mtlA"
                     /locus_tag="ACHKFK_001937"
     CDS             16905..18818
                     /gene="mtlA"
                     /locus_tag="ACHKFK_001937"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO9052286.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPVVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAAARRVQEMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            19238..20386
                     /gene="mtlD"
                     /locus_tag="ACHKFK_001938"
     CDS             19238..20386
                     /gene="mtlD"
                     /locus_tag="ACHKFK_001938"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO9052287.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGTLPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            20386..20973
                     /gene="mtlR"
                     /locus_tag="ACHKFK_001939"
     CDS             20386..20973
                     /gene="mtlR"
                     /locus_tag="ACHKFK_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO9052288.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(20984..21193)
                     /gene="yibT"
                     /locus_tag="ACHKFK_001940"
     CDS             complement(20984..21193)
                     /gene="yibT"
                     /locus_tag="ACHKFK_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO9052289.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRQLYRPKQVEEK"
     gene            21477..21839
                     /gene="yibL"
                     /locus_tag="ACHKFK_001941"
     CDS             21477..21839
                     /gene="yibL"
                     /locus_tag="ACHKFK_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO9052290.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            22398..23081
                     /locus_tag="ACHKFK_001942"
                     /pseudo
     CDS             22398..23081
                     /locus_tag="ACHKFK_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23125..27894
                     /locus_tag="ACHKFK_001943"
     CDS             23125..27894
                     /locus_tag="ACHKFK_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HFO9052291.1"
                     /translation="MNKIFKVIWNPATGSYTVASETAKSRGKKSGRSKLLISALVVGG
                     LLSSGVQASTALDGGGASESTPLTDTWIAIGQGAKASSVDTSPSGGPGTSSIAIGQEA
                     QSGIGSTSLGFKSQATGERSVALGQTADATGNRAIAIGSGAKSTADYSLTLGNGSKAF
                     AENATAIGKDARAEGKGSQAFGKVALATGENAIALGSNSNASGDNAVALGSTSQAYGD
                     SSLAIGELSETNAENAIALGKLSNASKINSIALGSNSTASGVGSVALGENSFAGGINS
                     LALGSQSNANGDNAVALGVGSVAEQDNTVSVGNSTTQRKITNMAAGLILPGSTEAING
                     SQLHALSASVAARLGAGAGVNGDGSINAPSYKLKSGTYNNVGEALLGIDNDTLHWDKT
                     EGAFSASYIVKNADGSPKSSIAQNKIINVAKGTISATSTDVVNGSQLYDLQQDALLWN
                     GTAFSAAHGSDTTSKITNVAKGTVSATSTDVVNGSQLYDLQQDALLWNGTAFSAAHGT
                     EATSKITNVEDGTISDTSKDAVNGSQLKETKDDVATNTANIATNTTDIATNTANIADN
                     TADIATNTTDIATNTANIATNTTDIATNTADIATNASSITTLNADVDTLEKDALLWNG
                     TAFSAKHGTEATSKITNVTAGDLTAGSTDAVNGSQLKTTNDNVSTNTTNITNLTDSVG
                     DLKDDSLLWNKTAGAFSAAHGTEATSKITNLLAGKVSSDSTDAINGSQLYGVADSFTS
                     YLGGGADISDTGVLTGPTYTIGGTDYTNVGDALAAINTSFSSSLGDALLWDATAGKFS
                     AKHGINNDPSVITDVANGAVSSTSSDAINGSQLYDVSDYIADALGGNAVVNADGSITT
                     PTYAIAGGSYDNVGDALEAIDTTLDDALLWDETANGGNGAFSAAHGKDKTASVITNVA
                     NGAVSATSSDAINGSQLYSTNKYIADALGGDAEVNADGTITAPTYTIANTDYNNVGEA
                     LDALDNNALLWDEDAGAYNASHDGNASKITNVAAGDLSTTSTDAVNGSQLNATNILVT
                     QNSQMINQLAGNTSETYIEENGAGINYVRTNDSGLAFNDASAAGIGSTAVGYNSVADG
                     DNSVAIGHGSNSIVETSIALGSESVSSRVIVKGSRNTSVSEEGVVIGYDTTDGELLGA
                     LSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGAVATTPTKYYHANSTAEDSLAVG
                     EDSLAMGAKTIVNGNAGIGIGLNTLVLADAINGIAIGSNARANHADSIAMGNGSQTTR
                     GAQTNYTAYNMDAPQNSVGEFSVGSEDGQRQITNVAAGSADTDAVNVGQLKVTDAQVS
                     QNTQSITNLNTQVTNLDTRVTNIENGIGDIVTTGSTKYFKTNTDGADANAQGKDSVAI
                     GSGSIAAADNSVALGTGSVADEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSS
                     EAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVSAGVNNNDAVNYAQLKQSVQETK
                     QYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAV
                     ALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQW"
     gene            28306..29961
                     /gene="lldP"
                     /locus_tag="ACHKFK_001944"
     CDS             28306..29961
                     /gene="lldP"
                     /locus_tag="ACHKFK_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO9052292.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            29961..30737
                     /gene="lldR"
                     /locus_tag="ACHKFK_001945"
     CDS             29961..30737
                     /gene="lldR"
                     /locus_tag="ACHKFK_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO9052293.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGEKEKIQLCFEATLSEDPDLASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGDHNEHSREKNA"
     gene            30734..31924
                     /gene="lldD"
                     /locus_tag="ACHKFK_001946"
     CDS             30734..31924
                     /gene="lldD"
                     /locus_tag="ACHKFK_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO9052294.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQVLGKELPAALAPMAK
                     GNAA"
     gene            31972..32445
                     /gene="trmL"
                     /locus_tag="ACHKFK_001947"
     CDS             31972..32445
                     /gene="trmL"
                     /locus_tag="ACHKFK_001947"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO9052295.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(32498..33319)
                     /gene="cysE"
                     /locus_tag="ACHKFK_001948"
     CDS             complement(32498..33319)
                     /gene="cysE"
                     /locus_tag="ACHKFK_001948"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO9052296.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(33399..34418)
                     /gene="gpsA"
                     /locus_tag="ACHKFK_001949"
     CDS             complement(33399..34418)
                     /gene="gpsA"
                     /locus_tag="ACHKFK_001949"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO9052297.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERERCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(34418..34885)
                     /gene="secB"
                     /locus_tag="ACHKFK_001950"
     CDS             complement(34418..34885)
                     /gene="secB"
                     /locus_tag="ACHKFK_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO9052298.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(34948..35199)
                     /gene="grxC"
                     /locus_tag="ACHKFK_001951"
     CDS             complement(34948..35199)
                     /gene="grxC"
                     /locus_tag="ACHKFK_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO9052299.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(35340..35771)
                     /gene="yibN"
                     /locus_tag="ACHKFK_001952"
     CDS             complement(35340..35771)
                     /gene="yibN"
                     /locus_tag="ACHKFK_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO9052300.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKNLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            36016..37560
                     /gene="gpmM"
                     /locus_tag="ACHKFK_001953"
                     /gene_synonym="pgmI"
     CDS             36016..37560
                     /gene="gpmM"
                     /locus_tag="ACHKFK_001953"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO9052301.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFTALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            37594..38853
                     /gene="envC"
                     /locus_tag="ACHKFK_001954"
     CDS             37594..38853
                     /gene="envC"
                     /locus_tag="ACHKFK_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO9052302.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALSERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGSPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            38857..39816
                     /gene="yibQ"
                     /locus_tag="ACHKFK_001955"
     CDS             38857..39816
                     /gene="yibQ"
                     /locus_tag="ACHKFK_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO9052303.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPLEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASAN"
     gene            complement(39803..>39876)
                     /locus_tag="ACHKFK_001956"
     CDS             complement(39803..>39876)
                     /locus_tag="ACHKFK_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052304.1"
                     /translation="RLWQWRDKTRSHHRITRSAFNLR"
BASE COUNT         9716 a        10519 c        10330 g         9311 t
ORIGIN      
        1 ttgtattatt tttattaata tcattcttcg atatggattc cacacaagta tattatcgat
       61 tgatgaatta tcccgacttt tatttgtctg gttaaccttt attggcgcaa tcgttgctta
      121 tatggacaac agccatgtgc aggttacttt tttagtcgaa aaactctcac cggctaatca
      181 acaacgtgtt tcgctgctaa cccatacgct cattttattg ctgtgtctgg ggctggcctg
      241 gggcgctata gaaaaaacag cgcaagactg gagcaactta tcgcccattc ttggcgtccc
      301 cattggctta atgtacgccg ccgctatccc caccagtctg attatcgcat tgctggaatt
      361 gcgtcactta taccggcagt tcacaaatac ccccagccga aaccaacaag gagcctgacg
      421 atgacaatcc ttatatttat tgtcagtttg ttaggggcta tcgcaattgg cgttcccatc
      481 gcctgggcat tattactgtg cggcatttca ttgatgttct ggatggatat ctttgatgtg
      541 caaattctgg cacaaacgtt ggtaaatggc gcagacagct tctccctgtt agccatcccc
      601 ttctttgtac tggcgggtga gatcatgaac gcgggcggcc tgtcgcaacg tatcgtcgac
      661 ttgccaatga aactggtagg ccacaggcca ggcggtctgg gttacgtggg cgtgctggca
      721 gcgatgatca tggcaagcct gtccggttct gcggttgctg ataccgcagc tgtcgcggcg
      781 ctgctggtgc cgatgatgcg ccaggccaac tatccggtaa accgcgccgc cgggcttatc
      841 ggttccgggg ggattattgc ggccattatc ccgccttcca taccactcat tatcttcggc
      901 gtctccagcg gactctccat cagcaaactg tttatggcgg gcatcgcacc gggcatcatg
      961 atgggcgtca cgctgatggt tacctggtgg tggcaggcaa aacgactgaa tttaccttgt
     1021 cagccgaaag cctcactgcg agaagtctgg caatccttag tttccggtat ttgggcgctc
     1081 tttttaccga taattattat tggcggtttc cgttcggggt tatttacacc gaccgaagca
     1141 ggcgcggttg ccgccttcta cgctctgttc gtctcggtgg tggtctatcg cgagatgacc
     1201 ttctccacgc tttatcatgt gctgattaac gcggcaaaaa ccacatccgt ggtgatgttc
     1261 ctggtggcct ctgctgcggt atctgcatgg cttattacga tcgccgaatt acccatgatg
     1321 gtatcggagc tattacaacc gttggttgat tcgcctcgct tattattcat cgtcattatg
     1381 ctcgctatta tggtgatcag tacggtcatg gacttaacgc ctactgtatt aattcttaca
     1441 ccagtattaa tgccattagt gaaagaagcg ggaatagatc cggtctattt cggcatcatg
     1501 tttattatca actgttcaat atcactgatt acgcctccag tcggtaatgt tcttaacgtg
     1561 gtatgcggcg tggcgaaact taaatttgac gatgcggtaa aaggcgttgc gccttacgtc
     1621 atggttttat tcatgttgtt agcattattt atttttatac cggagctcat taccgctcct
     1681 cttaaatgga tgagttaaaa ggaaatcata atgaaattac gttctgtaac ctacgcttta
     1741 ttaattgccg gagtggcgac gttcagcaca tcttctctgg cggcacaatc attacgtttc
     1801 ggttatgaaa cctcacaaac cgattcacaa catattgcgg cgaaaaaatt taatgaatta
     1861 ctgcaggaaa aaaccaaagg cgaactgaaa ttaaaactgt tcccggatag cacccttggt
     1921 aatgcgcagg cgatgatcag cggcgtgcgt ggcggcacta ttgatatgga gatgtctggc
     1981 tctaacaact ttacgggact ctcgcccgtc atcaacctgc tcgatgtccc gttcctgttc
     2041 cgcgataccg ctcacgcgca taaaacgctc gacggcaaag tcggtgatga cctgaaagtc
     2101 tcccttgaag gtaaggggct gaaagtgctg gcttactggg aaaacggctg gcgcgatgtc
     2161 accaactcgc gtgcaccggt caaaacgcct gccgacctga aagggttgaa aatccgcacc
     2221 aacaacagcc cgatgaatat cgccgcattt aaagtttttg gcgctaaccc gatcccgatg
     2281 ccgtttgccg aagtctatac cgggctggaa acccgcacta tcgacgctca ggaacacccg
     2341 atcaacgtcg tctggtcagc aaaattttat gaagtgcaga agtacctttc tctgacccat
     2401 cacgcctatt cgccgcttct ggtggtgatc aacaaagcga agtttgatgg cttaaccccg
     2461 gagttccagc aagcactgat ctcatctgca caggaagcgg gtagctatca gcgcaaactg
     2521 gtcgccgaag atcagcaaaa aatcatcgac ggtatgaaag aagcgggcgt ggaagtcatc
     2581 accgatctcg accgcaaagc ctttagcgat acgctgggta cccaggtccg tgacatgttt
     2641 gttaaagatg ttccgcaggg tgctgatctg ctgaaagccg tggatgaggt gcaataaacc
     2701 atgacgcaat actggctggg gttagattgt ggcggtagct ggctgaaagc cgggctgtat
     2761 gaccgagaag gccgggaggc gggcgtgcag cgcctgccgc tgtgcgcatt aagtccacag
     2821 ccaggccggg cagagcgcga catggcagaa ctgtggcaat gttgcactgc tgtcattcgc
     2881 gccctgctta cccattccgg cgtcagcggg gaacaaattg tcggtatcgg catctccgcg
     2941 cagggaaagg gcttatttct acttgataaa aacgacaaac cgctcggtaa cgccattttg
     3001 tcctcggatc gccgggcgat ggaaatcgtt cgtcgctggc aggaagatgg catcccggaa
     3061 aaactctatc cgctgacccg acaaacctta tggaccgggc atccggtgtc gctgttacgc
     3121 tggctgaaag agcacgaacc agaacgctac gcgcaaattg gctgcgtgat gatgacgcac
     3181 gactacctgc gctggtgttt aaccggcgtc aaaggttgcg aagagagcaa tatttctgag
     3241 tccaatctct acaacatgag tcgcggggaa tatgacccgt gccttaccga ctggctgggg
     3301 attgctgaaa tcaatcacgc cctgccgcct gttgtcggat ctgccgaaat ctgcggggaa
     3361 atcaccgctc agacagccgt actgaccggt ctgaaagcgg gtacccccgt tgtcggcggc
     3421 ctgtttgatg tggtttccac cgcactctgc gccgggctcg aagacgaatt caccctcaat
     3481 gcggtgatgg ggacctgggc ggtgaccagc ggcataaccc acggtttacg tgacggtgaa
     3541 gcgcatccgt atgtctatgg tcgctacgtt aacgatggtc aatttatcgt tcacgaagcc
     3601 agccccacct cttccggcaa tctcgaatgg tttaccgcac agtggggaga aatctcattt
     3661 gctgagatta atcaggccgt tgccagtttg ccgaaagccg gtggcgatct ctttttcctg
     3721 ccgttcctgt acggcagcaa cgccggactc gaaatgacca gcggtttcta cgggatgcag
     3781 gccattcaca cccgtgcaca cctcttacag gctatctatg aaggggtggt gttcagccat
     3841 atgacccatc tcaaccggat gcgcgaacgt tttactgatg tgcacaccct gcgcgtcact
     3901 ggcggcccgg cgcactccga tgtctggatg caaatgctgg cggacgtcag cggtctgcgt
     3961 atcgagctgc cgcaggtgga agaaaccggc tgctttggtg cggcacttgc cgcccgcgtc
     4021 ggtaccgggg tttatcgcga tttcagcgaa gcccaacgtg acttacagca cccggtacgc
     4081 accctgctgc cgaatatggc cgcgcatcag ctttaccaac aaaaatacca acgctatcaa
     4141 catctcattg ccgcacttca gggttatcac gcccgcatca aggagcacac attatgagtc
     4201 gaccacttct gcaactggcc ctcgaccact catcacttga agccgcgcag cgcgacgtga
     4261 cgctgttaaa agacagcgtc gatattgtcg aagcaggcac cattctctgt ttaaacgaag
     4321 ggcttggcgc agtgaaagcc ttgcgcgaac agtgccctga caaaatcatc gttgccgact
     4381 ggaaggtcgc cgacgcgggt gaaacgctcg cgcaacaggc atttggcgca ggcgctaact
     4441 ggatgaccat catctgcgcc gcgccgctcg ccacggtgga aaaaggccac gcgatggcac
     4501 agaactgcgg cggtgaaatt cagatcgagc tattcggcaa ctggacgctg gacgatgccc
     4561 gtgactggca tcgtattggt gtacgacagg cgatttatca tcgtggtcgt gacgcgcagg
     4621 ccagcgggca acagtggggc gaggccgatc tggcacgcat gaaagcgctt tccgatatcg
     4681 gtcttgagct ttccattacc ggcgggatta cccctgctga cctgccgctg tttaaagaga
     4741 tccgcgtgaa agcgtttatt gccggacggg cgctggcagg cgcagcaaat ccggcgcaag
     4801 tggccgctga ttttcatgcg caaatcgact ccatctgggg aggcaagcgt gcgtaatcat
     4861 ccgttaggca tttatgaaaa agcgctggcg aaagatctct cctggccgga gcggctggtg
     4921 ctggcaaaaa gctgtggttt tgattttgtc gaaatgtcgg tggatgaaac cgacgaacgg
     4981 ttgtcacgtc ttgactggag cgccgcacaa cgtgcttcgc tggtcactgc gatgatcgaa
     5041 acgggtgttg ctattccgtc aatgtgcctg tccgcacatc gtcgttttcc ttttggtagc
     5101 cgggacgacg ccgtgcgcca gcgggcgcgg gagattatga gtaaagcgat tcgcctggcg
     5161 cgcgatctcg gcattcgtac tattcaactg gcaggttacg acgtctatta cgaggatcat
     5221 gacgaaggca cccagcaacg atttgctgaa gggctggcgt gggcggttga acaggcagcg
     5281 gcatcacaag tcatgctggc ggtggagatt atggacaccg cgtttatgaa ctctatcagc
     5341 aaatggaaaa agtgggacga aatgcttgcg tcaccgtggt tcaccgttta cccggacgta
     5401 ggcaatctca gcgcctgggg caatgatgtt cccgccgaac tgaaactggg catcgaccgt
     5461 atcgccgcca tccacctgaa agacactcaa cccgttaccg aacatagccc cggacagttc
     5521 cgcgatgtgc cgtttggcga aggctgtgtc gattttgttg gcatcttcaa aacgctgcat
     5581 gagctgaact atcgcggatc atttctgatt gaaatgtgga ccgaaaaagc caaagagccg
     5641 gtgctggaga ttattcaggc gcggcgctgg attgaagcgc gtatgcagga ggccggattt
     5701 atatgttaga gcaactgaaa gctgacgtgc tggcggcaaa cctggcgctt cccgttcatc
     5761 atctggtgac gttcacctgg ggcaatgtca gcgcgataga cgaaacgcgg caatggatgg
     5821 tgattaagcc ttccggcgtt gagtatgagg taatgaccgc cgacgatatg gtggtggttg
     5881 agatagctag cggcaaggtg gtggaaggca gcaaaaagcc ctcttccgac acgccaacgc
     5941 atctggcgct ttaccgtcgc tttgcggaga ttggcggcat agtgcatacc cactcgcgcc
     6001 atgccaccat ctggtcgcag gccggacttg atctccccgc ctggggcact actcacgccg
     6061 attattttta cggagccatc ccctgcacgc gcctgatgac cgcagaagag atcaacggcg
     6121 agtatgaata ccagaccggc gaagtgatca ttaaaacctt cgaagagcgt gggttgaacc
     6181 cggcacaaat ccctgcggtg ctggtgcatt ctcacggccc gttcgcctgg ggaaaaaatg
     6241 ccgccgatgc ggtgcataac gccgtggtac tggaagagtg cgcctatatg ggtctgttct
     6301 cgcgccagct tgcgccacag ctccctgcga tgcaaaacga actgctggat aagcactacc
     6361 tgcgtaagca tggggccaat gcctattacg ggcagtagcc atacctgata gtgcccggca
     6421 atacacgccg ggcagccttt cacatcagaa cagccccaac ggtttatccg agtagctcac
     6481 cagcaggcac ttggtttgct ggtaatgctc aagcatcatt ttgtgggttt cgcggccaat
     6541 gcctgattgt ttgtagccgc caaacgccgc atgtgccggg taagcgtgat aacagttggt
     6601 ccacacgcgc ccagcctgaa tgccgcgccc catcttatag gccagattac cgttgcggct
     6661 ccagacgccc gcgcccaggc catattgcgt atcgttcgcc agctccagcg cttcttctat
     6721 cgttttgaag gtggttaccg ccagcaccgg gccaaaaatc tcctcctgga acacgcgcat
     6781 attgttctga ccaaacaaaa tcgtcggttc gaggtagtag ccgtctttca gttcgccttc
     6841 cagcagcttg cgccgcccgc cggtgagcac atcagcgccc tcttttttac cgatatcaat
     6901 gtagttgagg atggtttcca gttgcccgtg agaaacctgc gcgcccattt gcgtcacgct
     6961 gtcgagcggg ttaccgctgc gaatgctttc gacacggcgg atggcgcgtt ccataaagcg
     7021 ttcgtagata gactcctgca ctaaagcacg gcttggacag gtgcaaactt cgccctggtt
     7081 aaaggcaaac agtgcaaagc cttccagcgc tttatcgaaa aaggcatctt cttcatccat
     7141 cacatcagca aagaagatat ttggcgattt gccgcccagc tccagcgtca ccggaataat
     7201 gttttgcgtg gcgtattgca taatttgttg gcccacttcc gttgagccgg taaacgccac
     7261 tttggcgatg cgtttcgagg tcgccagata ttcgccaatt tccccacctg cgccgttgac
     7321 cacgttcacc acccctggcg gcagtaaatc accgacgatt tccattagca gcagtacaga
     7381 aagcggagta agacgtgcgg gtttcagcac cacacagttg ccagccgcca gcgcgggggc
     7441 cattttccag ctcgccatca gcagcgggaa gttccacgga ataatctgcc ccaccacgcc
     7501 taacggttcg tggaagtgat aagccacagt ttcgctatcc acttcactaa taccgccttc
     7561 ctgcgcccga atacacgagg caaaatagcg aaaatggtca atcgccagcg gcacatcagc
     7621 agcactggtt tctcgaatgg gtttgccgtt atcccaggtt tcagcggtcg ctaacagttc
     7681 gaggttctgc tccatccgat cggcaatctt aaacagaatc gccgcgcgat cctgcaccga
     7741 agtatgcgcc catttatctt tcactttgtg cgcagcatcc agcgccagat cgatgtctcg
     7801 tttgcccgaa gacgccactt cgcacagcag ctgcccggtc accggagtca ggttctggta
     7861 atactcgccg tcggcagggg ctacccactc gccgccgata aagttgtcat aacgggcttt
     7921 taacttaaga ggaaaaccat actcgccggg cttaatctgt gctgaagggg gattattggt
     7981 catgatcgtc tccttgcggt gtgaggtata acaagggtag acgtgactgg cgaaatcttc
     8041 gccagtcgat aacagcttta cgacggctat cacgaattta cgggcaaggg ttacagcgga
     8101 taatgcgcga agtacacggc ttgctggtca ttaattttct tctcttgcgc aaacccttct
     8161 ttaacaattg tccgtaaatc agctcgagcc gtattatcag agacgcccag gctcgcgcta
     8221 atttcagcaa ctgtgtactc tttccccgga ttggcgagca tcacattcag gagcgttacc
     8281 tggcgcggat tcaggcgttt aagcacacca gagctaaaca gcacactatc gagttttgcg
     8341 cttcgttgtg taatttcatc gatatgctgc tgccagtttt gcagagcacg tttaatgaca
     8401 cccgcctgat attcaaggaa ataagtcaga tccataccat ctgattcagt gtactggtag
     8461 ctggcggcat atttgaccgg tgcagcatga agcaaacggc tgatggaaat atatttaaag
     8521 acatcgtagc ccgatttcag catataccag taaaacagcg cccgactggt gcgcccgttg
     8581 ccatcccgaa acggatgttc atgggccagc ataaaatgca gaatgcaggc acggaccaac
     8641 ggatgaatat acccttcatg gctgtttaac cactggcaaa ctttttccag ccgttccggt
     8701 aacaatgccg cagcgggcgg ttgatgcact atattgccat catagtcggc gatcacaaca
     8761 tcatctgttt cacgaaattc tcccggacga tatttcgcgt cattgattcc acccatacca
     8821 atggcatgta actggcgaat aagcgcaggt gtcagcggtt ctgcgagtaa gtgcggaatt
     8881 tccgtcatca gccgggcatt tcccgcaatc atatgctcgt cttcagtgcg tggcgaccgc
     8941 cctgtttcca gcatttttct cgccaccagc gttgtagtat tcgcaccttc cagctgggag
     9001 ctggtgatcg gctcttccag gcgtaactgg cttaactcgg ctccggcccc gcgtaaccgt
     9061 tcagtctgat ctttcagcgc cagcgcggta gcacgtttgt cgacaagctc gcacgcttct
     9121 gcaatcaccg gggtaatgtt gaatccggcc tgttcacccg cttcattgca ataattaatt
     9181 cgttggatcg ccgcgttgcg cgccagacgg gtaagcgtcc acgccacatc gacgttttct
     9241 ccttttttga tgcggtactg caactcatca aaaggcagat aacgtccttt ttcatcggta
     9301 acccggttaa agcgcaggtg ggccaggagt tcctcacccg ataagtgtga taaaggaata
     9361 agtccgggag gtgatttgat agccatgtgc ttatctcaaa aaagcgcaat attgagatag
     9421 tgtatagcag gaaagagaaa ctatctcaaa aaaagcaagt tttgagatag ttgaaaaagc
     9481 ggctgacaac tcgccagccg ttgtggaaat gatgattaca tcgctgcgcg ataaatcgcc
     9541 acaatttctt cgtgagttgc ctggatcggg ttagtaaagc cgcaggcatc tttcagggca
     9601 ttagtcgcca gaaccgcgaa atcttcttct ttcacgttca ggtcgcgtag gcctgccggg
     9661 atatccactt tcttcgccag ttcacggatg gcgttaatgc aggcttcagc accttctgcg
     9721 tcgtttttac ctgtcacgtt cacgcccatt gcagcggcac aatcacgcag acgtgcagcg
     9781 gcgactttgc tgttgaatac ctgaacatgc ggcagcaaaa cggcgttaca tacaccgtgt
     9841 ggcaggttgt agaaaccgcc cagttggtgc gccatcgcat gaacataacc cagagaagca
     9901 ttattgaacg ccataccggc gaggaactgg gcataagcca ttgcttcacg cgctttagca
     9961 ttgctgccat cttcaacggc taacggcagg ttttcggcaa tcatggtcac ggctttcagt
    10021 gcacaagcgt cagtgatcgg cgtggcggca atagaaacat acgcttcgat agcgtgcgtt
    10081 aaggcatcca tacccgttgc agcggtcagt gacttcggca taccgatcat cagagaggag
    10141 tcattgacag aaagcagcgg agttacatgc ttatccacaa tcgccatttt gatgtgacgc
    10201 gcttcgtcag tgatgatgca gaaacgggtc atttcagagg ccgtacccgc cgtggtgttg
    10261 atggcgatca tcggcagctg cggttttgca gagcggtcaa cgccttcata atcgcggata
    10321 tcaccgccgt tggctgcaac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg
    10381 cctaaagaaa tcacgctatc gcaattattt tctttaagta atttcaaacc tgcagcgacg
    10441 ttttccgtgg tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct
    10501 tccagtgctt tttgcacatc acccgccata cccaatttcg ttaacatgtt gtcggtgaca
    10561 attaaggtac gggtaaatcc ataatctgcc atcatattca ttgcatcatt caatgaatca
    10621 gcaccaataa cattcacgga aggaataaaa aaggttgaag ctgccataac acactcctga
    10681 aagtgtaaaa aagggcgggc agcttactga ggattttcat ttgaaaatat gatcaacatt
    10741 accgtttatt aaaatatttt taataaaaag cacgttacat tcatcaaata acgtgccttc
    10801 ttaacattaa taagaaattt tgtattaatt catatttatt atgaattaat catttcctta
    10861 tttttccgga aataataatg cgtcgcgcaa taaatgatca tttccacgac gacgcgtaaa
    10921 gccaatacgg tcaaaatatt ccagaatctg aattgccagc ttgcggccta cgcctaagcg
    10981 atcgcggaaa tccgccgcgc aggttgaacc gcattcctga tcgagatcgc ggatcatatt
    11041 ggcaaattcg acaatccgat cgttacggta ataacgatct ttgacgatcg cagtaattat
    11101 cccctgttgc gccgcctggc gtagcgtcag gcgcattgcc tgctcgtcgg ttcccgtctc
    11161 ttttgccagg tcacgcaccc accacggctc gtcaccaaac agcggctctg ctttttgcca
    11221 gatggcctgc tgctctgcac tgaagcccgc tttatgatcc ggcagatgca gccagccgtg
    11281 atgactgtgg atgtcgccgc tttcgcgcat cttttcaatc agcaacagta ccagcgcttc
    11341 atcttccatt ggcaacgcca tacgtcgcag acgttcgcgc ccaggaccag gttcatcgcg
    11401 atgttgctca tgataagtcg ctaatgtgtc gagaattttc cgctgccagc gggcggcaac
    11461 tggcgcattc aacaagctgt aaccagcctg aatataacca ggctgttgca gcagttcgcg
    11521 catcccttcg ccgttgagct gccgtgccca ggcgaaatcc gcaaggttaa ccgcgccgcg
    11581 ttccagatga acagataacg catcggcatc gctctgcgcc cgtgcaagag acgccagcca
    11641 ttgcagatat tccggcttac gcttaccgcg gcgcggcggg ttaagcatca acacacgcgc
    11701 ccccgccagc gtgttgcggg ccgagatatc gcgcagcact aagcgatctt tatccgccag
    11761 ccacagcggc gtgtcgaaca ccagctcggc aaggttatct tccagcagcg aaacgcgtcc
    11821 cgtaacgtgg ctggcggcgt ggtgaatatg cagcggctgc cactgggtca gcggtgtatg
    11881 ggtttgaagc tcgacaatca cccgtgtgaa cggctccggc ggcacatcgg caagcagcca
    11941 gtcgccacgg ttaatctgct ctttttccgc atcacccgcg atgttaagcg cgatacgctg
    12001 cccggcatgg gcagtttctg ttggctgatt ttgcgcatgc agcgcacgca cgcgcatcgg
    12061 tttatttaca ccagtcagcc agagtgaatc gcctactttc acttccccgc ttaacgccgt
    12121 accggtgacg accagcccgg caccttttac ggtaaatgcg cggtcgatcg cgaggcggaa
    12181 actatgttgg ctggcgtgtt cacgttctgg taactgaagc agatgctcgc gcagggcatc
    12241 aattccccga ccttcggttg ctgcggtgat aaacagtttt gcctcagcaa aaccgtattc
    12301 ccgcagcacc tcctttacct ggcgttcaac ctcatcaaca cgcgcttcgt ccacgcgatc
    12361 ggctttggtc agcgccactg tcagcatcgg gttaccggtc agctgcaaaa tcgccagatg
    12421 ctcacgggtc tgtgccatca caccgtcatc gcacgccacc accaacagcg tgtgatcgat
    12481 accaccaacg cccgccagca tgttggaaag aaacttttca tgaccgggca cgtcgataaa
    12541 accaggcacg cgaccatccg gctgcggcca gtaggcatag ccgagatcga tggtcatgcc
    12601 gcgctttttt ccttccggca gacggtcggc atttacgccc gtaatcgcct gcaacaaggt
    12661 tgttttgccg tggtcaacgt gtccggcagt cgcaataatc atttcaacaa catctccaaa
    12721 aaccgttgct catcttcaag gcagcgtaaa tccagccaca atcgtccgtc ataaatacga
    12781 ccaatcaccg gcactggcaa ttcacgccag cgggcggcta atgactcaag gtggctaccg
    12841 cgtccatcat ggggggtaaa cgttaatgcc gcgctcggca ggcgatcaac cggcagcgaa
    12901 ccactgccga tctgcgaaag acatggcata acctgtaccg caaactccgc gccgtaatgt
    12961 gcggcaaggg gagcctgtaa acgttgtgcc tggatttgaa tgacctctgc gctgcgggta
    13021 agcaggcgca gggtcggtaa tttttcactc agagcttcag ggtgtaaata aagacgcaac
    13081 gtggcttcca gcgccgcgag ggtcatttta tccgcgcgta atgcacgctt cagcgggtgg
    13141 ctttgcaggc gggcgatcat ttctttttta ccaacaataa ttcctgcctg cggcccgcct
    13201 aacagcttgt cgccggagaa gctcaccaga ctgacgcccg ccgcaatcaa ctcctgcggc
    13261 attggctctt tcggcaaacc gtactggcta agatcgacca gcgagccact gcctaaatca
    13321 gtcaccactg gaacatccag ttctttgccg agcgccacca gttccgcttc atctatcgct
    13381 ttggtgaacc cctgaatgct gtagttactg gtatgtactt tcatcaacag tgcggtattt
    13441 tcattcaccg cctgacgata atcattcgcg tgcgtgcggt tggtggtccc tacttcgtgt
    13501 agggtgcatc ctgcctgacg cataacatcg ggaatacgaa acgcgccgcc aatctccacc
    13561 agttcgccgc gagaaaccac cacctctttc ccgctggcag tggccgccaa catcaataac
    13621 accgccgccg cattgttatt aacgatacag gcatcttccg cccccgtaat acggcacagc
    13681 agctgcgcca gcgcccgatc gcgatgtccg cgtccggcgt cgtccagatc gtactcgagg
    13741 gtcactggcg aacgcatagc cttcgtaacg gcttccaccg cggcttccgc ctgtaaagct
    13801 cgcccaaggt tggtatgcag cacggttcct gtcaggttga ttaccggacg cagcgcgctt
    13861 tgcgcctctt tcgtcaaccg ggcatcgact tcttgcgccc agttttcaca ccacgcgggc
    13921 agcgtctggc tgtcacgaat cacttctcgc gcttcgtcaa gcatctgacg cagcaattcc
    13981 accacgcggg tgtgaccata agtatcacgc aaagaaagga aggtgctatc gcgcaataag
    14041 cgatcaatag ccggaagttg actatagagg gaacgcgttt cggttgtcat aggaaacctg
    14101 gctgatcaag gccctctcac acggagaagg gcgtttaaca taaccacgga ttgtaacgtg
    14161 agatgggtca ggaggacata tcgcgcatca agcctttggc ggttcggtgc gagcaaaact
    14221 ttcgcggcta aacaggtttt cgaccagttt gactaagtgt gggcggtcga cacaccagcc
    14281 tggcgcaacg cggcggaaat tgagatatcc gacggcacag gcaatggcga tagtcgccag
    14341 attgaccgta tcggttttga gcgtgccatc gaccagatat ccttccagca catccagact
    14401 gcggttgatt ttctcacgct gacgtaacaa ttcatcttca gactgctgcg ccgccggacg
    14461 cgcctgttca cgcaccgata ccagcccggc atccataatg ccatccgcca gtgcctcaat
    14521 tttgcgcacc cgcaacgact ccagcggatc gcgcggcaac atcgccggag caacattcat
    14581 taattcaata tattcagcga tgatcggtga atcaaaccag cattcgccct cttcggtcac
    14641 cagcaccggc acttttccta acgggttaaa ttgcgccacg ccgttatccg cgttataggg
    14701 cagttcatta ataaattcga acgttatgcc cttttctaac aacagaatag aaagtttgcg
    14761 tacaaacggg ctggtgtagc taccgacgag tttcatgccg agtcctttgt gcgaggaaaa
    14821 atatcagtat ggctcaggct cgcgcaaaac acaggtgata caagtctcaa tgatccaaat
    14881 aaagataatg catccaactg gtcattctta gcagcacttt ccgcatcacc gaaacatggc
    14941 tgaaatggtc ggagtaaact gccacctggg cgtagatgcc gtcgggtaag gcatcgatat
    15001 cagcgtcagg gtccagttca atggttccca gcacaccgtc cgtgccgggc acgaccgtta
    15061 atgattgcaa taccccctgc gcctgataag aaccgcctgg cacgacaggt aaaatactgg
    15121 tcagtttgcc gtgaaacacc tgcccaggta gcgcgttaaa caccacttcc gcatcatcgc
    15181 cgggtttcag acgtaacagc gagttctgcc gaaattgggc gacaatttgc cgtttttgct
    15241 cggggatgaa aaccatcacc ggacgtagcg gcaaggcagc tgcgtatgta cctgggcgga
    15301 tcagtacctg agtgacgtag ccattgctcg gtgcgcgaat gacggtctgc tcaaggttat
    15361 attttgcttc agtaagttgc gctcttaagc tcacaatctg cgattgctcg ccgttaacca
    15421 tactgtcgag ctggctctgg atctgcgcct gctccgccac cgagcctttc accagcgcat
    15481 cctgcgcgag gaaattttgc cgcgcatcgt cgatgtcacg ttccgagaac ggattcaccg
    15541 ccgcctggct gcctttcagg taacgttgat aatttttaaa cagacggtcg cgctccgctg
    15601 aaacctgggt ggtgttggcc tgcgcttcag tgagctgcgc gcgcagcgtc tttatattat
    15661 gcgtcgccgt catcaggtca gcctgaagcc tgtcaactcg cgcctggtaa cgaaccgggt
    15721 cgagcttaaa aagcacctcg cccttctgaa taagctgatt atttttgtcg gtgacttcag
    15781 taacaattcc cgtcacctgt ggcgtgatgg ggatcgctat cactgccttt tgcgcggtaa
    15841 aagtgtaagg gtggttgtag ttcatcaaca aaatcaaacc actcaccaga aacacgcctc
    15901 ccagcgccgc cgtcgccagc gtccactgat tcaccggaat gcggaagatt ttaaagaccg
    15961 cccacgccag cgccacgtaa gttaaaacaa tcaatagatc cataattaga tctccggaaa
    16021 cgtggaatag tcagtctttt tctcggcggc gagttggtgc tccagccggg aaatccggtc
    16081 agaaagtgcg gcgatttccg ggtctggcac gtcgccttgc ggcgcaacat gtgactgcat
    16141 gccccagccg cgctccggtt gatagagcgt cgcccagatc cacaggaacg gccagataac
    16201 atgcagagta aacaggctca cccagcccgc cgtatgaata gcgtcggcat ggggatggtt
    16261 gcgctttttg gcaatcagat aagggatgtc atgtatcgcg atgattccat aaaaaatcac
    16321 cagaaagacg aagatcagca ctcccaacgc gaaatagttt agaaacatat ctgcctcgga
    16381 ttcacgttta tcagtgttgt tttttggctg gcagccagaa gggagtcagg ctgatatttt
    16441 gacaataatc cgggttcgcg attctcgcca taacaccaaa gaataatttt aagaggtgat
    16501 gagttgctta gttacataac gatttcatga cgaaggcata acatgctgta gatcacatca
    16561 ggtgaacgcc gtaagaaaat atcttgtgat tcagatcaca aagattcaac aaatcatcaa
    16621 aataaaaatg tgatatcact cacatttaaa tgccattttt agcgaaaatc gccgccttgt
    16681 tgctttttta cacaagcatt ttgtgatgag cgtcacgtca attacctctc taccccctac
    16741 atttatgtga ctgatatcac acaaaaggcc accgactgga cagttaaccg attcagtgcc
    16801 agatttcgca gtatctacaa ggtccggcta cctctgccgc cacattaaca aaaaacctcg
    16861 ggcttccagc ctgcgcgaca gcaaacataa gaaggggtgt ttttatgtca tccgatatta
    16921 agatcaaagt gcaaagcttt ggtcgtttcc tcagcaacat ggtgatgcca aatatcggcg
    16981 cgtttatcgc gtggggtatc atcaccgcgt tatttattcc aacagggtgg ttaccgaacg
    17041 aaacgctggc gaagctggtc gggccgatga tcacttatct cctgccgctg ctgatcggtt
    17101 ataccggtgg taagctggta ggcggcgaac gtggcggcgt agtcggtgcc atcaccacca
    17161 tgggcgttat cgtcggcgca gatatgccga tgttcctcgg ctctatgatt gcaggtccgc
    17221 tgggcggctg gtgcattaag cacttcgacc gctgggtaga cggtaagatc aaatccggtt
    17281 ttgaaatgct ggtgaataac ttctccgcag gcatcatcgg gatgatcctc gctattctgg
    17341 catttctcgg cattggcccg gttgttgaag ccctgtctaa aatgctggcg gcgggcgtta
    17401 acttcatggt tgtccatgac atgctgccgt tggcgtctat ctttgttgaa ccggcgaaaa
    17461 tcctgttcct caacaacgct attaaccacg gtatcttctc gccgctgggt attcagcagt
    17521 cccatgaact gggtaaatcc atcttcttcc tgattgaagc taacccaggt ccaggtatgg
    17581 gcgtgctgct ggcgtacatg ttctttggtc gtggtagcgc taaacagtct gcgggcggtg
    17641 cggcaatcat ccacttcctg gggggtatcc acgaaatcta cttcccgtat gtgctgatga
    17701 atccgcgtct gattctggcc gttatcctcg gcggtatgac tggcgtgttc acgctaacta
    17761 tcctgggcgg tggtctggtt tctccggcat ctccgggttc tatccttgct gtactggcga
    17821 tgacaccaaa aggtgcatac ttcgctaaca tcgcgggtgt gtgtgcggcg atggcagtct
    17881 ccttcgttgt ctctgccatt ctgctgaaaa ccagcaaagt gaaagaagaa gatgatattg
    17941 aagctgctgc acgtcgcgtt caggaaatga aagctgagtc taaaggcgca tctccgctgt
    18001 ctgcgggcga tgtgactaac gacctgagcc acgtacgtaa aatcatcgtt gcctgtgatg
    18061 ctggtatggg ttccagtgcg atgggtgcag gcgtgctgcg taagaaaatt caggatgcag
    18121 gtctgtcgca gatttcggtc actaacagcg cgatcaacaa cctgccgcca gatgtggacc
    18181 tggtcatcac tcaccgtgac ctgaccgaac gcgctatgcg ccaggttccg caggcacagc
    18241 atatttcgct gaccaacttc ctcgacagcg gcctgtacac cagcctgacc gaacgtctgg
    18301 ttgctgccca gcgccatact gaaaacgaag tgaaagtgaa agacagtctg aaagacagct
    18361 ttgacgattc cagtgctaat ctgttcaaac tgggcgcgga gaacatcttc ctcggtcgca
    18421 aagcggccac caaagaagaa gcgattcgtt ttgctggcga gcagctggtg aaaggcggtt
    18481 acgttgagcc ggaatacgtt caggcgatgt tggatcgtga aaaactgacc ccgacttatc
    18541 tgggtgagtc tatcgcggtg ccacacggta cggttgaagc gaaagatcgc gtactgaaaa
    18601 cgggcgtcgt gttctgccag tacccggaag gcgtgcgctt cggtgaagaa gaagatgaca
    18661 ttgcccgtct ggtgattggt attgctgccc gtaacaacga gcacattcag gttatcacca
    18721 gcctgaccaa tgcactggat gatgagtccg tcatcgagcg tctggcacac accaccagcg
    18781 tggatgaagt gctggaattg ctggcaggtc gtaagtaatc cattcccacc ctctccgcat
    18841 gaagaaggtg gggctaattg cctgatgcgc ttcgcttatc aggcctacaa cataaagcac
    18901 aatttgtttt gtcggtcgga taaggtgttt acgccgcatc cggcagtcgg tgcacaatgc
    18961 ctgatgcgac gctgacgcgt cttatcaggc ctacaggtca gtgccaaaca cgtaggccgg
    19021 ataaggcgtt tacgccgcat ccggcagtcg gtgcgcaata cctgatgcga cgctgacgcg
    19081 tcttatcagg cctacaggtc agtgccaaac acgtaggccg gataaggcgt ttacgccgca
    19141 tccggcaaaa agcccctctc ctcacggaga gggtttgggt gagggaaaag cctcacccca
    19201 gccctctcgg gtaaaaacat tgatgaaggt taatactatg aaagcattac attttggcgc
    19261 aggtaatatc ggtcgtggct ttatcggtaa actgctggcg gacgcgggta tccaactgac
    19321 gtttgccgat gtaaatcagg tggtacttga tgccctgaat gcccgtcata gctatcaggt
    19381 tcatgtggtt ggtgaaaccg agcaggtaga taccgtttcc ggcgtcaatg ccgtcagcag
    19441 cattggcgat gatgtcgttg atctgattgc tcaggtcgat ttagtcacta ccgccgttgg
    19501 ccctgtcgtg ctggaacgta ttgctccggc catcgccaaa gggctggtga aacgtaaaga
    19561 acaaggtaat gaatctccgc tgaacatcat cgcctgtgaa aacatggtcc gcggcaccac
    19621 gcagctgaaa ggccatgtga tgaacgccct gccggaagat gccaaagcgt gggtagaaga
    19681 acacgttggc tttgttgatt ccgccgttga ccgcatcgta ccgccttcgg cttcggcaac
    19741 taacgatccg ctggaagtga cggtagaaac cttcagcgaa tggattgtcg ataaaacgca
    19801 gttcaaaggc acgctgccaa acatccctgg catggaatta accgacaatc tgatggcatt
    19861 tgtcgaacgt aaactcttca ccctgaatac aggtcatgct ataaccgcgt acctcggaaa
    19921 actggccggt catcagacca ttcgtgacgc aattctcgac gagaaaatcc gcgcggtggt
    19981 aaaaggggca atggaagaaa gcggcgcggt actgatcaag cgctacggct ttgatgctga
    20041 caaacatgcg gcatacatcc agaaaatcct cggtcgtttc gagaacccgt acctgaaaga
    20101 tgatgtagaa cgcgtaggcc gtcagccgct gcgtaaactg agtgctggcg accgtctgat
    20161 caagccactg ctcggaaccc tggaatatgg tctgccacat aaaaacctga ttgaaggtat
    20221 tgccgctgca atgcacttcc gcagtgaaga tgacccgcag gctcaggaac tggcagcgct
    20281 gattgccgac aaaggcccac aagccgcgct ggcacagatt tccggtcttg atgccaacag
    20341 cgaggttgta tccgaggcgg taaccgctta taaagcaatg caataatggt ggaccaggcg
    20401 caggataccc tgcgcccgaa taacagattg tcagatatgc aggcaacaat ggaacaaacc
    20461 caggcctttg aaaaccgtgt gcttgagcgt ctgaatgctg gcaaaaccgt gcgaagcttt
    20521 ctgatcaccg ccgtcgagct tttgaccgag gcggtaaatc ttctggtgct tcaggtattc
    20581 cgcaaagacg attacgcggt gaagtatgct gtagaaccgt tactcgacgg cgatggtccg
    20641 ctgggcgatc tttctgtgcg tttaaaactc atttacgggt tgggcgtcat taaccgccag
    20701 gaatacgaag atgcggaact gctgatggca ttgcgtgaag agctaaatca cgacggcaac
    20761 gagtacgctt ttaccgacga cgaaatactt ggaccctttg gtgaactgca ttgcgtggcg
    20821 gcgttaccac cgccgccaca gtttgaacct gcagactcca gtttgtatgc aatgcaaatt
    20881 cagcgttacc aacaggctgt gcgatcaaca atggtccttt cactgacaga gctgatttcc
    20941 aaaatcagct taaaaaaagc ctttcaaaag taagtacttt cgcttacttc tcctctacct
    21001 gtttcggacg ataaagctga cgataatact ccagacgttg tagatataac ggcacgcttt
    21061 catcaggtat tccggacgga atcgcgttac ggggaggtag ttttttcaga tactcccgga
    21121 acgcctggct ggatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
    21181 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
    21241 tttagtttag atcccggtat aaatgacatt aaggaaaacg tgctcaatcc tcaataatca
    21301 cctgcttctg ttgactatct tttttcttta aatttcttaa ctaatcaatg tattgggtga
    21361 ttttttaacg cattaatggt tacaatctgg aactattaca atgccatcag ttgtgaacaa
    21421 agcacctggt cgcgcatact agaggctata aatttatctc tatcagaagt catcacatga
    21481 aagaagtcga aaaaaacgaa atcaaacgtc tcagcgatcg tctggacgcc atccgtcacc
    21541 agcaggccga tctgtcgctg gttgaagccg cagacaaata tgccgaactg gaaaaagaga
    21601 aagccacgct ggaagcagaa attgctcgcc tgcgcgaagt tcacagccag aaactcagca
    21661 aagaagcgca gaaactgatg aagatgccgt tccagcgtgc aatcaccaaa aaagagcaag
    21721 ctgatatggg caaactgaaa aaaagtgttc gcggactggt ggttgtgcac ccaatgaccg
    21781 cgctgggccg tgaaatgggc ctgcaagaga tgacagggtt ctcaaagact gcgttttaag
    21841 ttcctttctc gtcgctgtaa tcacagcctc tcaccattta ctcgctcctg ctggcacatg
    21901 caggagcctg tttcagagat cgaatcttta taacgttctt aacattatat attcaactcc
    21961 acctacactt ctgagcgatc gtagcaggct aaattctgaa aatcaggtac acattaaaga
    22021 atgatgtatc aatatcaaca atgaatttcc gcatatcaat gctcgaaaca taattaatta
    22081 tttaaatttt tcaatcagct tcgcaaattc cacaataaaa catcactcaa ctgaatattt
    22141 acctttccct gccctgtgcc tttatccggt gaaacatttg acatcgattg acaccgatca
    22201 ttaaaatccg cgcgattaat attattggct acaaactgaa atcgtttgac atttaataac
    22261 atattcgcac aaccaattaa tacaaaaact ctctaacaaa tttattttgc ggatatgcct
    22321 cattccaatc attcataaaa aaaatgattg tgccgtaatt atcaactgta tcgggattat
    22381 gaaaaggaat tcatcctatg aacctaaaga aaacactgtt aagcgtgtta atgatattgc
    22441 aactttgctt attggtaggg tgtgactata ttgaaaaagc gagtaaggtc gacgatctcg
    22501 ttacacagca agagttacaa aaaagcaaaa ttgaggcgct tgaaaaacaa caagaactcg
    22561 acaagcgcaa gatagaacac tttgaaaaac aataaactac catcataaac agtaccaaaa
    22621 cgctcgctgg cgtgatgaag gcagttaaag acaaacagga cgaatttgtc tttacagaat
    22681 ttaacccggc acaaacccaa tacttcattt taaataacga ttctgttggt ttggcaggga
    22741 aaatactgtc tattgacgca gtagaaaacg gcagcgttat tcgtatttca ctggttaact
    22801 tattaagtgt tcctgtatca aatatgggtt tttacgcgac atggggggga gaaaaaccca
    22861 ccgacatcaa cgcattagca aaatggcagc aattgctatt tagtacctca atgaactcct
    22921 ccctgaaatt attaccaggt caatggcaag acattaattt gacgctaaaa ggtgtctcgc
    22981 ccaacaacct caaatatctg aaattagcca tcaacatggc aaatattcag ttcgaccgtc
    23041 ttcaacctgc tgaatctcca cagcggaaaa acaaaaaata aatgcagctt ctgcgcttat
    23101 atcaaggaat agagagcatc aataatgaac aaaatattta aagttatctg gaatccggca
    23161 acaggtagtt acaccgttgc cagtgaaaca gcaaaaagtc gtggtaaaaa aagcgggcgc
    23221 agtaagctgt taatttctgc gttggttgtg ggtgggttgt tgtcgtctgg agtacaagca
    23281 tcgaccgcgc ttgatggtgg tggggctagc gaaagcactc cattgaccga tacctggatt
    23341 gccattggtc agggtgctaa agcatcatca gtagacactt caccatcagg aggaccagga
    23401 acatcaagta ttgcaatagg gcaagaagcc caatctggga taggtagtac ctcattaggt
    23461 ttcaagagcc aggctacagg agaaagatct gttgctttag gacaaacagc tgacgcaacc
    23521 ggtaataggg ccatagcaat cggtagtgga gctaaatcga ctgccgatta ttcccttacg
    23581 ctggggaatg gatctaaggc tttcgctgaa aacgccacgg cgataggcaa ggacgcaaga
    23641 gcggagggca aaggtagtca ggccttcgga aaagtggcct tggccactgg ggagaatgcc
    23701 attgccttgg gtagtaacag caatgcatct ggtgataacg ccgttgcgct gggtagtaca
    23761 agtcaggcat atggcgactc cagccttgca ataggtgagt taagtgagac caatgctgaa
    23821 aatgctattg cactgggaaa actcagtaac gcaagtaaaa tcaattcaat cgccctgggt
    23881 agtaacagca ctgcatctgg tgtgggctcc gttgcgctgg gagagaatag ttttgctgga
    23941 ggcataaaca gtcttgcttt gggtagtcaa tccaatgcaa acggtgataa cgcagttgcc
    24001 ctcggtgtag gatctgtcgc agagcaagac aataccgttt cggtagggaa cagcacaaca
    24061 caacgcaaaa ttaccaatat ggctgccggt cttatactac caggcagtac tgaggccatt
    24121 aacggttcac aacttcatgc attaagtgcc tcagtcgcag cccgactcgg ggctggtgct
    24181 ggcgtaaatg gagatggaag tataaatgcg ccaagctaca agctgaaaag cggcacctac
    24241 aataatgtgg gagaggcgct tttgggaatc gacaatgaca ctctgcactg ggacaaaacc
    24301 gagggagcat ttagtgccag ctatatcgtc aaaaacgctg atggcagccc aaagagcagc
    24361 atagcccaaa acaaaatcat taacgttgca aagggtacta tttccgccac cagcaccgat
    24421 gtagtaaacg gctctcaatt gtacgacctg cagcaggatg ctctgttgtg gaacggcact
    24481 gcattcagcg ccgcacacgg tagtgatacc accagcaaaa tcactaatgt tgctaaaggt
    24541 acggtttctg caaccagcac cgatgtagta aacggctctc aattgtacga cctgcagcag
    24601 gatgcgctgt tgtggaacgg cacagcattc agtgccgcac acggcaccga agccaccagc
    24661 aaaatcacta atgttgaaga tggtactatt tcagacacca gtaaagatgc ggttaacggt
    24721 tcccagttga aagaaaccaa agacgatgtg gccaccaaca cagccaatat cgccactaac
    24781 accacggata ttgcaaccaa cactgccaat atcgccgata acactgctga tatcgccact
    24841 aacaccacgg atattgcaac caacactgcc aatatcgcca ctaacactac ggatattgcg
    24901 actaataccg ccgatatagc tacaaacgct agcagtatca ctactctgaa cgcggacgtt
    24961 gataccctgg aaaaagatgc cctgctctgg aatggcacag cattcagcgc caaacacggc
    25021 accgaagcta ccagcaagat caccaatgtt accgctggcg acctgactgc tggcagcact
    25081 gacgccgtta atggctccca gctcaaaacc accaacgata acgtgtcgac caacaccacc
    25141 aacatcacca acctgacgga ttccgttggc gaccttaagg acgattccct gctgtggaac
    25201 aaaacggctg gcgcattcag cgccgcgcac ggcaccgaag ccaccagcaa gatcaccaac
    25261 ttactggctg gcaaggtatc ttctgacagc accgatgcca ttaacggctc acaactttat
    25321 ggcgtagcgg attcatttac gtcatatctt ggtggtggtg ctgatatcag cgatacgggt
    25381 gtattaaccg ggccaaccta cactattggt ggtactgact acactaacgt cggtgatgct
    25441 ctggcagcca ttaacacatc atttagctca tcactcggcg acgccctact ctgggatgca
    25501 accgcaggca aattcagcgc caaacacggc attaataatg atcccagtgt aatcactgat
    25561 gttgcaaacg gtgcagtctc gtccaccagc agcgacgcca ttaacggttc gcaactttat
    25621 gacgttagtg actacattgc cgatgctttg ggcgggaacg ctgtggtgaa tgcagacggc
    25681 agtatcacta caccgactta tgccattgct ggcggcagtt acgacaatgt cggtgacgcg
    25741 ctggaagcga tcgataccac gctggatgat gctctgctgt gggatgagac agccaatggc
    25801 ggtaatggtg catttagcgc cgctcacggg aaagataaaa ctgccagtgt aatcactaac
    25861 gtcgctaacg gtgcagtctc tgccaccagc agcgatgcca ttaatggctc acagctctat
    25921 agcaccaata agtacatcgc tgatgcgctg ggtggtgatg cagaagtcaa cgctgacggt
    25981 actatcactg cgccgactta caccattgca aataccgatt acaacaacgt cggtgaagcc
    26041 ctggatgcgc tcgataataa cgctctgctg tgggatgaag acgcaggtgc ctacaacgcc
    26101 agccatgatg gcaatgccag caaaattacc aacgttgcgg ctggtgatct ctccacaacc
    26161 agtaccgatg ctgttaacgg ttcccagtta aacgcaacca atatcttggt tacgcaaaat
    26221 agccaaatga ttaaccagct tgctggtaac accagtgaaa cctacatcga agaaaatggt
    26281 gcgggtatta actatgtacg taccaacgac agcggcttag ctttcaacga tgccagcgca
    26341 gcaggtattg gctctaccgc tgtgggttat aactctgttg ccgatggcga taacagcgtg
    26401 gcaatcggtc atggcagcaa cagcatcgtt gagacgagta ttgcgctggg tagcgaatca
    26461 gtctccagcc gtgtgattgt taaaggttct cgtaacacca gcgtatcgga agaaggtgtt
    26521 gtgattggtt atgacaccac ggatggcgaa ctgcttggcg cgttgtcgat cggtgatgat
    26581 ggtaaatatc gtcaaatcat caacgtcgcg gatggttctg aagcccatga cgctgttact
    26641 gttcgccagt tgcaaaacgc cattggtgca gtagcaacca caccaaccaa atactatcac
    26701 gccaactcaa cggctgaaga ctcactggca gtcggtgaag actcgctggc aatgggcgcg
    26761 aaaaccatcg ttaatggtaa tgcgggtatt ggtatcggcc tgaacacttt agttctggct
    26821 gatgcgatca acggtattgc tatcggttct aacgcacgcg caaatcatgc cgacagcatt
    26881 gcaatgggta atggttctca gactacccgt ggtgcgcaga ccaactacac tgcctacaac
    26941 atggatgcac cgcagaactc tgtgggtgaa ttctccgtag gtagcgaaga cggtcaacgt
    27001 cagatcacca acgtcgcagc aggttcggcg gataccgatg cggttaacgt gggtcagttg
    27061 aaagtaacgg acgcgcaggt ttcccagaat acccagagca ttactaacct gaacactcag
    27121 gtcactaatc tggatactcg cgtcaccaat atcgaaaacg gcattggcga tatcgtcacc
    27181 accggtagca ctaagtactt caagaccaac accgatggcg cagatgccaa cgcgcagggt
    27241 aaagacagtg ttgcgattgg ttctggttcc attgctgccg ctgacaacag cgtcgcactg
    27301 ggcacgggtt ccgtagcaga cgaagaaaat acgatctctg tgggttcttc taccaaccag
    27361 cgccgtatca ccaacgttgc tgccggtgtt aatgccaccg atgcggttaa cgtttcgcaa
    27421 ctgaagtctt ctgaagcagg cggcgttcgc tacgacacca aagctgatgg ctctatcgac
    27481 tacagcaaca tcactctcgg tggtggtaac ggcggtacga ctcgcatcag caacgtttct
    27541 gctggcgtga acaacaacga cgcagtgaac tatgcgcagt tgaagcaaag tgtgcaggaa
    27601 acgaagcaat acaccgatca gcgcatggtt gagatggata acaaactgtc caaaactgaa
    27661 agcaagctga gtggtggtat cgcttctgca atggcaatga ccggtctgcc gcaggcttac
    27721 acgccgggag ccagcatggc ctctattggt ggcggtactt acaacggtga atcggctgtt
    27781 gctttaggtg tgtcgatggt gagcgccaat ggtcgttggg tctacaaatt acaaggtagt
    27841 accaatagcc agggtgaata ctccgccgcg ctcggtgccg gtattcagtg gtaatcatcc
    27901 attaacgaat gggtaaacgc cacattgcct gatgcgctac gcttatcagg cctacggggt
    27961 gattcagcac taccctgtag gccggataag gcgttcacgc cgcatccggc aagataaggc
    28021 gcaccagatc aacaatctga gggcaattct ctgatgggaa ttgccctttt cttcattacc
    28081 tctctcccag gattggccct accaattctt cattgatctg acctctggtt cacaattccc
    28141 caattaaaac tcacatcacc gttgccaata cataacattt ggttaactat tcattgtcat
    28201 tatccctaca caacacaatt ggcagtgcca cttttacaca acgtgtgaca aggagatgag
    28261 caacagactc attacacggc gtgtgtggac tgcaggagac ctgcaatgaa tctctggcaa
    28321 caaaactacg atcccgccgg gaatatctgg ctttccagcc tgatagcatc gcttcccatc
    28381 ctgtttttct tctttgcgct gattaagctc aaactgaaag gatacgtcgc cgcctcatgg
    28441 acggtggcaa tcgcccttgc cgtggctttg ctgttctata aaatgccggt cgctaacgcg
    28501 ctggcctcgg tggtttatgg cttcttctac gggttgtggc ccatcgcgtg gatcattatt
    28561 gcagcggtgt tcgtctataa gatctcggtg aaaaccgggc agtttgacat catccgctcg
    28621 tctattcttt cgataacccc tgaccagcgc ctgcaaatgc tgatcgtcgg tttctgtttc
    28681 ggcgctttcc ttgaaggagc cgcaggcttt ggcgcaccgg tagcaattac cgccgcattg
    28741 ctggtcggcc tgggctttaa accgctgtac gccgccgggc tgtgcctgat tgttaacacc
    28801 gcgccagtgg catttggtgc gatgggtatt ccgattctgg tcgccgggca ggtaacaggc
    28861 atcgacagct ttgagattgg tcagatggtg gggcgtcagc tgccgtttat gaccattatc
    28921 gtgctgttct ggatcatggc gattatggac ggctggcgcg gtatcaaaga aacctggcct
    28981 gcggtcgtgg tggcgggtgg ttcgtttgct atcgcacagt acctcagttc caacttcatt
    29041 gggccggaac tgccagacat tatctcttcg ctggtatcgc tgctctgtct gacactgttc
    29101 ctcaaacgct ggcaaccagt tcgcgtattc cgcttcggtg acttaggggc gtcacaggtt
    29161 gatatgacgc tggctcacac aggttacacg gcaggtcagg tgctgcgcgc ctggacaccg
    29221 ttcctgttcc tgaccgccac cgtaacgctg tggagtatcc cgccgtttaa agccctgttc
    29281 gcttcaggcg gtgcgctgta tgagtgggtg atcaacattc cggtgccgta cctcgataaa
    29341 ctggttgccc gtatgccgcc agtggtcagc gaagcgacag cgtatgccgc cgtgtttaag
    29401 ttcgactggt tctctgccac cggtaccgcc attctgtttg ctgccctgct ctcgattgtc
    29461 tggctgaaga tgaaaccatc tgacgctatc agcaccttcg gcagcacgct gaaagaattg
    29521 gctctgccta tctactccat cggtatggtg ctggcattcg cctttatctc gaactattcc
    29581 ggtttgtcat caacactggc gctggcactg gcgcacaccg gtcatgcatt caccttcttc
    29641 tcgccgttcc tcggctggct tggggtattc ctgaccgggt cggatacgtc atctaatgct
    29701 ttgtttgccg ctttgcaggc taccgccgca caacaaattg gcgtttctga cctgttgctg
    29761 gttgcagcca acaccaccgg tggtgtcacc ggtaagatga tctccccgca atctatcgct
    29821 atcgcctgtg cggcggtagg cctggtgggc aaagagtctg atttgttccg ctttactgtc
    29881 aaacacagcc tgatcttcac ctgtatggtg ggcgtgatca ccacgcttca ggcctatgtc
    29941 ttaacgtgga tgattcctta atgattgttt tacccagacg cctgtcagac gaggttgccg
    30001 atcgtgtgcg ggcgctgatt gatgaaaaaa acctggaagc gggcatgaag ttgcccgctg
    30061 aacgtcaact ggcgatgcaa ctcggcgtgt cgagaaattc actgcgcgag gcgctggcga
    30121 aactggtaag cgaaggtgtg ctgctcagtc gacgtggcgg cgggacgttt attcgctggc
    30181 gtcatgacac atggtcggag caaaacatcg tccagccgct gaaaacgctg atggccgatg
    30241 atccggatta cagtttcgat attctggaag cccgctacgc cattgaagcc agcaccgcat
    30301 ggcatgcagc aatgcgtgcc acgcctggcg aaaaagaaaa gattcagctt tgttttgaag
    30361 caacgctcag cgaagacccg gatctcgcct cacaagcgga cgttcgtttt catctggcga
    30421 ttgccgaagc ctcacataac atcgtgctgc tgcaaaccat gcgcggtttc ttcgatgtcc
    30481 tgcaatcctc tgtgaagcat agccgccagc ggatgtacct ggtaccccca gttttttcac
    30541 agctgaccga acaacatcag gcggtcattg acgccatttt tgccggtgat gctgacgggg
    30601 cgcgtaaagc aatgatggcg caccttagct ttgttcacac caccatgaaa cgattcgatg
    30661 aagatcaggc tcgccatgca cgcattaccc gcctgcctgg tgaccataat gagcattcga
    30721 gggagaaaaa cgcatgatta tttccgcagc cagcgattat cgcgccgcag cgcaacgcat
    30781 tctgccgccg ttcctgttcc actatatgga tgggggggca tattctgaat acacgctgcg
    30841 ccgcaacgtg gaagatttgt cagaagtggc gctgcgccag cgtattctga aaaacatgtc
    30901 tgacttaagc ctggaaacga cgctgtttaa tgagaaactg tcgatgccgg tggcgctagg
    30961 tccggtaggt ttgtgtggca tgtatgcgcg acgcggcgaa gttcaggctg ccaaagccgc
    31021 ggatgcgcat ggcattccgt ttactctctc gacggtttcc gtttgcccga ttgaagaagt
    31081 cgctccagct atcaaacgtc cgatgtggtt ccagctttat gtgctgcgcg atcgcggctt
    31141 tatgcgtaac gcactggagc gagcaaaagc agcgggttgt tcgacactgg ttttcaccgt
    31201 ggatatgcca acgccgggag cgcgttatcg tgatgcgcat tctgggatga gcggcccgaa
    31261 cgcggcaatg cgccgctatt tgcaggcggt gacgcatccg caatgggcgt gggatgtggg
    31321 cctgaacggt cgtccgcatg atttaggtaa tatctcggct tacctcggca aaccgaccgg
    31381 actggaagat tacatcggct ggttggggaa taacttcgat ccgtccatct catggaaaga
    31441 ccttgagtgg atccgcgatt tctgggatgg cccgatggtg atcaaaggga tcctcgatcc
    31501 ggaagatgcg cgcgatgcag tacgttttgg tgctgatgga attgtggttt ctaaccacgg
    31561 tggccgccag ctggacggtg tgctgtcttc tgcccgtgcg ctgcctgcta ttgcagatgc
    31621 cgtgaaaggt gatatcgcca ttctggcaga tagcgggatt cgtaacgggc tggacgttgt
    31681 gcgtatgatc gcgctcggtg ctgataccgt actgctgggc cgcgctttcc tgtatgcgct
    31741 ggcaacagcg ggccaggcgg gtgtagctaa tctgctaaat ctgatcgaaa aagagatgaa
    31801 agtggcgatg acgctgactg gcgcgaaatc gattagcgaa attacgcaag attcgctggt
    31861 gcaggtgctg ggtaaagagt tgccggcggc attagctccg atggcgaaag ggaatgcagc
    31921 ttaagggtta gacgaatatc tgctatcctg cccccgcact aagggggcag tatgctaaac
    31981 atcgtacttt acgaaccaga aattccgcca aatactggca acatcatccg tctttgcgct
    32041 aataccggct ttcgtctgca tatcatcgaa ccgatgggat ttgcctggga cgataagcgc
    32101 ctgcgccgcg cggggctgga ctatcacgag tttaccgccg ttacgcgtca tcatgactat
    32161 cgcgcgtttc tcgaagcaga aaatccccag cgcctgtttg ccctcaccac gaaaggtacg
    32221 cctgctcaca gcgccgtaag ctatcaggat ggcgattatc tgatgttcgg cccggaaaca
    32281 cgcggcctgc cagcgagcat tcttgatgcc ctgcccgctg aacaaaaaat tcgtattcca
    32341 atggtgccgg acagtcgcag catgaacctg tccaatgcgg tgtcggtcgt ggtgtatgaa
    32401 gcctggcggc agttggggta tcctggagcg gtgttgagag attagttact gtaggccgga
    32461 tagatgatta catcgcatcc ggcacgagca caggacatca gatcccatca ccatactcaa
    32521 acgtatggtt aatgccgttg aaatgctgat ccatatccat tgacggttta tcgctatccg
    32581 gcttgccaac aatgcgtgcc ggaacgccag cggcagtagt gtgcggcggc accggttgca
    32641 gcaccacgga acctgcgcca atcttcgcgc cgcgcccaac ttcaatattg ccgagaattt
    32701 tcgcgcccgc gccaatcatc acaccttcac gaattttcgg gtgacggtcg ccaccagatt
    32761 tacccgtacc gccaagcgtc acagattgca gaatcgatac gtcgttttca atcaccgccg
    32821 tttcacccac aacgatgccc gtcgcgtggt cgagcatgat accgcgacca atttttgccg
    32881 ccgggtgaat atcaacctgg aacgttacag agacctggtt ttgtagaaaa atagccagcg
    32941 cgcgacggcc ctgattccac aaccagtgac cgatgcgata cgcctgcaaa gcatgaaaac
    33001 ccttcagata tagcaacggg gttgagtatt tatccaccgc cgggtcacgg gtacgcaccg
    33061 cctgaatatc acaggccgca gaggcgatca tttccgggtc ggcggcgtag gcttcttcca
    33121 ccacttcacg gatagcaata gccggcataa tgggcgatga cagcttgttc gccagcatgt
    33181 agctcagtgc actgccaagg ttttcgtgct tgagtagcgt cgcgtggtaa aaactggcca
    33241 gcattggctc acagtccgcc agcgttctgg cttcagcttt aatattgttc cagacaattt
    33301 ccagttcttc acacgacatt gcttactcca cacgataaga taatgaccga cccgttctgc
    33361 gcgggccggg tcatagtggt aacaaaggtt ccctgggttt agtggctgct gcgctcgtcc
    33421 ttgcgtgcac gacctagtaa agtcaatgct gcctcgcgcg cgttttttcc gcaatataat
    33481 acttgataaa tttcctcggt tattggcatt tcaacgccga agcgatgcgc cagttcgcgg
    33541 acttctttcg tattgcggta gccttcaacc acctgaccaa tcttctcctg cgcgctttgt
    33601 acatccatgc cctgaccgag catcatgcca aaacggcggt tacgcgactg gttgtcggta
    33661 caggtaagca ccagatcgcc cagccccgcc atgcccataa aggtggcagg atcggcaccc
    33721 agcgccgcac caagacgcga catttcagcc agcccacggg tgatcagtgc cgtacgcgca
    33781 ttcgcaccaa aaccgatacc gtcggacatc cccgcgccaa tggcaatgac gtttttcacc
    33841 gcgccgccaa gctgcacacc gatgaaatcc gggttgctgt aaacgcggaa acttttgcca
    33901 cagtgcaaca attgctggag atcatcggca aaggtctggt cggtagaggc cagcgaaatg
    33961 gccgttggta gacctgccgc cagttctttc gcaaacgttg ggccagagat aaccgccaac
    34021 ggaatttgat cgcctaaggc ctcacgcgcc acgtcctgta acagacgtcc ggtttctgct
    34081 tccagccctt tggtcgccca caccagacgc gcatcaggac gcatcagcgg tttaatctga
    34141 cgcagcactt caccaaagac atggctgggt acgacgacga gaatattacg gctggctgcc
    34201 agcgcagtgg cgagatcgct ttcaagatgg agcgtatcgg gaaaaggcac atcggggaga
    34261 aacgcggcgt tacagcgctc gcgttcaagc gttgcgatat gttcagggtc atggccccag
    34321 aggacaacct cgtggccatt tcttgccagg gtaatggcaa gagcggtgcc gtacgagccg
    34381 gcaccgatca cagtcattga agcattacgt tggttcatca ggcatcctga tgttcttcag
    34441 taccttcgcc agcctgctgc tgcaaatagt tcatgaacag cgcatcgaag ttaaccggcg
    34501 caaggttcag ttgcgggaat gtaccgcggg ataccatgct ggtgatgcac tcacgagcat
    34561 acgggaacag aatgttcggg cagtatgcgc ccaggcaatg tgccatctgg gtgccttcga
    34621 tacccgcgat ggagaaaata ccgccctgct gaacttcaca caggaacgcg gtttcttcgc
    34681 ccagggaggc cgttacggta acacgcagta ccacttcgta aacgtcatct gccagttgag
    34741 tagatgccgt atccagatca agtttaactt ctggttgcca atctttctgg aaaacgtgcg
    34801 gcgcgttcgg cgcttcgaaa gaaatatcct tggtataaat acgttggatc tggaaagtca
    34861 tttcggtgtt gttttgttct gacatgtgta gaaaaccctt aagtgttgtc cttaaatact
    34921 gcgcaatgta atgccaacgt tcacacgtta tttcagcagg ggatccagtc caccacgtgc
    34981 atccaatgca tacaagtcat cacaaccgcc aatgtgctgt gcgtcaataa aaatctgggg
    35041 aaccgtggtg cgaccgctgc gtttgatcat ctcttcacgc ttagcggcgt tgccatcgat
    35101 cggcaactcc tgaaaactca cgcccttgct gctcagcagt gcttttgcac gatggcaata
    35161 cgggcaggtt tctttggtat agatttcaac attggccata aattagctcc tgttttttac
    35221 cctgaaattt catgttgcag ggcggcagca aaatggagtg tccccagaag cttatatcaa
    35281 taagtcgctg gaggcctaat tgcagctaac ggcctgcatc atgaaagacg acagataaat
    35341 tatttgccgc gcaccaaagg caagttttcg ccagcccagc cagcgacgcc ttctttcagt
    35401 acgaatactt gcgcaaaacc agctttcgtc agcgcgtttg caggctcctg gcactgcatg
    35461 ccagaaccat ctaccacgat aaccggtttg tctttgtgct tctcaagctc accaacattg
    35521 ttggctttga tttcgctcgg caacaggttg atagaacctg cgatatggcc tttacggaag
    35581 tcatcacgct gacgtaaatc cacaaccaca gcgtcttctt tgttgatcag acgcgtagct
    35641 tcaccacgag taatcacctt cactttcgag gtcaggttct taaacgtagt cacaagaacc
    35701 gccaccagta acgcgatcca ggcgatactc agtatgggat gacggccaac aaattgcata
    35761 atttcttgca tggggggtaa caactcccga cgtagtgatt aaaaaaacca ggaaaggagt
    35821 atacctgcgc gttgcggcaa atacagccag cgcgttaact ggaatgcaat tttgcggggc
    35881 gctacgaaaa aaaagcgtaa atctggcatc tgcttgccgc gagcggtcgt atattttgat
    35941 cttcagaggc tattttatcg attcagctgt agtaaaatta cgcaaatttt gactcttgag
    36001 tatgaggttg tcgcaatgtc ggtttctaaa aaacctatgg tactggtgat tctggatggc
    36061 tatggctatc gcgaagaaca gcaggataac gccattttta gtgctaaaac cccggtaatg
    36121 gatgcactgt gggccaatcg tccgcatacc ctgatcgacg cttccggttt ggaagtcggt
    36181 ctgcctgacc gtcagatggg caactccgaa gtaggccacg ttaacctggg tgccggccgc
    36241 atcgtgtatc aggacctgac tcgtctggac gttgaaatca aagatcgagc tttctttgct
    36301 aatccggtgt tgactggtgc agtagataaa gcgaaaaacg caggcaaagc agtacacatt
    36361 atgggtctgc tctccgcagg tggtgtacac agccacgaag atcacatcat ggcgatggta
    36421 gaactggcag ctgaacgcgg cgcagaaaaa atctacctac acgcattcct tgacggtcgc
    36481 gacactccgc cgcgcagtgc tgaatcctcg ctgaaaaaat tcgaagagaa atttaccgcg
    36541 ctgggtaaag gccgcgtagc gtccatcatt ggtcgttact acgcgatgga ccgcgataac
    36601 cgttgggatc gcgttgaaaa agcttatgac ctgctgactc tggcgcaagg tgagttccag
    36661 gccgataccg ccgttgccgg tttgcaggct gcttatgctc gcgacgaaaa cgacgagttc
    36721 gtgaaagcga ccgttattcg tgcagaaggt cagccagatg cggcaatgga agacggcgat
    36781 gcactgattt tcatgaactt ccgtgctgac cgcgcgcgtg aaatcactcg tgcattcgta
    36841 aatgccgatt ttgacggctt cgcgcgtaag aaagtggtta acgtcgattt cgtgatgctg
    36901 accgaatacg ctgccgacat caaaaccgca gtggcttacc cacccgcttc cctggttaac
    36961 accttcggcg aatggatggc gaaaaacgac aaaactcagc tgcgtatttc cgaaaccgaa
    37021 aaatatgccc acgttacttt cttcttcaac ggtggcgtag aagagtcgtt caaaggcgaa
    37081 gatcgcattc tgatcaactc accgaaagtg gctacctacg atctgcaacc ggaaatgagc
    37141 tccgcagagc tgaccgaaaa actggttgcg gcaatcaaga gcggcaaata cgacaccatt
    37201 atttgtaact atccgaacgg cgacatggta ggtcacaccg gagtgatgga agcggcggtt
    37261 aaagcggttg aagcgctgga tcactgcgtg gaagaagtcg cgaaagcggt tgagtccgtg
    37321 ggtggacaac tgctgatcac cgctgaccac ggtaacgctg aacagatgcg cgatccggca
    37381 acgggtcagg cacacacagc acacaccaac ctgccagttc cgctgattta cgttggtgat
    37441 aagaacgtga aagcggttga aggcggcaaa ctttctgaca tcgcgccgac catgttgtcg
    37501 ctgatgggta tggaaatccc gcaagagatg actggtaagc cgctgttcat cgtggaataa
    37561 tccctcccca tgaggggaaa ggcgattaat accatgacac gggccgtgaa accgcgcagg
    37621 tttgcaatca ggcccatcat ctacgccagc gttctgagcg ctggcgtatt gttgtgcgcc
    37681 ttttccgccc acgcggatga gcgtgaccaa ctcaaatcca ttcaggccga tatcgccgcg
    37741 aaagagcgcg cggtacgcca aaagcaacaa caacgcgcaa gcctgctcgc acaattgaaa
    37801 aagcaggaag aagcgatctc tgaagccact cgcaaactgc gcgaaacgca aaatacgctc
    37861 aatcaactga ataaacagat tgatgagatg aacgcgtcga ttgccaaact ggagcagcaa
    37921 aaagccgccc aggagcgcag cctcgccgcg caactggatg ccgcgtttcg tcagggtgaa
    37981 cataccggta ttcagctgat tctcagcggt gaagaaagcc agcgtgggca gcggttacag
    38041 gcttatttcg gctatctcaa ccaggcgcga caagaaacca ttgctcagtt gaaacaaacg
    38101 cgtgaagaag tcgctatgca gcgtgccgaa ctggaagaga aacaaagcga gcaacaaacg
    38161 cttttatatg agcagcgcgc ccaacaggcg aagctgaccc aggcgttgag cgagcgtaaa
    38221 aagacgctgg cagggctgga gtcttccatc cagcaaggtc agcaacagtt gagcgagctg
    38281 cgcgccaacg aatcccgcct gcgtaacagc attgcccgtg cggaagctgc ggcgaaagcg
    38341 cgtgctgaac gtgaagcgcg cgaagcccag gcggttcgcg accgccagaa agaagcgacg
    38401 cgcaaaggca ccacctacaa gccgaccgaa agcgaaaaat cgctgatgtc ccgtaccggt
    38461 ggtctgggct caccgcgcgg tcaggcattc tggccagttc gcgggccgac gctgcatcgc
    38521 tatggcgaac agctacaggg tgaattacgc tggaaaggga tggtgattgg tgcctctgaa
    38581 ggtactgaag ttaaagcgat tgccgacggt cgggtgattc tggctgactg gctgcaaggc
    38641 tacggtctgg tggtggtggt tgagcatggt aaaggcgaca tgagtcttta cggctataat
    38701 cagagcgcac tggtgagcgt tggttcgcag gttcgcgcgg gccagccaat tgcactggtg
    38761 ggcagcagtg gcggtcaggg tcggccttca ctctatttcg aaattcgccg ccagggtcag
    38821 gcggtcaatc cacagccgtg gttgggaaga taagttttgt ttccatttcg tcgtaacgtt
    38881 cttgcatttg ccgctctgtt ggcgctctcc tcccccgtaa ttgctggcaa acttgccatc
    38941 gtcattgatg attttgggta tcgcccgcac aacgaaaacc aggtgctggc gatgccttcc
    39001 gcgatctccg tcgctgtatt acccgattca ccgcacgcca gagagatggc gaccaaagcg
    39061 cataacagcg gtcacgaagt gttgattcat ctaccgatgg cgccactgag taaacagccg
    39121 ctggagaaaa atacgctacg cccggagatg agcagcgacg aaattgagcg cattattcgt
    39181 agtgcggtca ataacgtgcc ctatgccgtg gggatcaaca accacatggg cagcaagatg
    39241 acctctaacc tgtttggtat gcagaaagtg atgcaggcgc tggagcgtta caatctttac
    39301 ttcctcgaca gcgtaaccat cggtaatacc caggcgatgc gcgccgcgca aggcactggc
    39361 gtgaaggtga tcaaacggaa ggtgttcctc gacgattcgc agaacgaagc ggacatccgt
    39421 gtgcaattta atcgcgcaat tgatctggcg cgtcgcaacg gttcaaccat tgccattggg
    39481 catcctcacc cttcaacggt acgcgtgcta caacagatgg tttataacct gccgccagac
    39541 attacgctgg tgaaagccag cagcttgctg aatgaaccgc aggttgatac gtctacaccg
    39601 ccgaaaaaca ctgtgcccga cacaccgcgt aatccattcc gtggcgtgaa gctgtgcaaa
    39661 ccgaagaagc cgctggaacc tgtttatgct aatcgcttct ttgaagtatt aagcgaaagc
    39721 atcagccaga gcacgctgat cgtttacttc cagcatcagt ggcaaggctg gggcaaacag
    39781 cccgaagcgg caaaacttaa cgctagcgca aattaaaggc gctacgcgta atgcgatggt
    39841 gcgaccgcgt tttgtcgcgc cattgccata agcgga
//
LOCUS       DAZGJT010000034        39680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZGJT010000034 DAZGJT010000000
VERSION     DAZGJT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.034"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..374
                     /locus_tag="ACHKFK_001957"
                     /pseudo
     CDS             84..374
                     /locus_tag="ACHKFK_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308825.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
     gene            557..2818
                     /gene="ybhJ"
                     /locus_tag="ACHKFK_001958"
     CDS             557..2818
                     /gene="ybhJ"
                     /locus_tag="ACHKFK_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO9052305.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVAALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGSISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGADDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSEMADVFARIKQIAGQEHVDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPNFDVGDYIYIPGIKAALDNPRTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(2959..4242)
                     /gene="ybhC"
                     /locus_tag="ACHKFK_001959"
     CDS             complement(2959..4242)
                     /gene="ybhC"
                     /locus_tag="ACHKFK_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO9052306.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDKVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEVQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(4394..4870)
                     /gene="ybhB"
                     /locus_tag="ACHKFK_001960"
     CDS             complement(4394..4870)
                     /gene="ybhB"
                     /locus_tag="ACHKFK_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO9052307.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPVGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            4979..6109
                     /locus_tag="ACHKFK_001961"
     CDS             4979..6109
                     /locus_tag="ACHKFK_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO9052308.1"
                     /translation="MKRAYKYRFYPTTEQAELLAQTFGCVRFVYNSILRWRTDAYYER
                     KEKIGYLQANARLTALKKEPEYIWLNDVSCVPLQQSLRHQQAAFANFFAGRAAYPAFK
                     SKRHKQVAEFTASAFKHRDGELYIAKSKSPLDVRWSRELPSAPSTVTISRDSAGRYFV
                     SCLCEFEPVSMPVTAKTVGIDVGLKDLFVTDTGFKTDNPRHTAKYAKRLTLLQRRLSR
                     KQKGSRNRIKARLKVARLHAKIADCRMDNLHKLSRKLINENQVVCVESLKVKNMIRNP
                     KLSKAIADAGWSELVRQLQYKGKWAGRSVVAIDQYLPSSKCCSCCGFTMQKMPLNVRK
                     WHCPECGADHDRDINAARNIKAAGLAVLAHGEPVNPESQHAA"
     gene            complement(6220..7509)
                     /gene="bioA"
                     /locus_tag="ACHKFK_001962"
     CDS             complement(6220..7509)
                     /gene="bioA"
                     /locus_tag="ACHKFK_001962"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO9052309.1"
                     /translation="MTTDDLTFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDRDGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQH
                     QVAAIEAQLREQLAPACDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            7596..8636
                     /gene="bioB"
                     /locus_tag="ACHKFK_001963"
     CDS             7596..8636
                     /gene="bioB"
                     /locus_tag="ACHKFK_001963"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO9052310.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVREAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            8633..9787
                     /gene="bioF"
                     /locus_tag="ACHKFK_001964"
     CDS             8633..9787
                     /gene="bioF"
                     /locus_tag="ACHKFK_001964"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO9052311.1"
                     /translation="MIWQEKIDAALDARRVADALRRRYPVAQGAGRWLVADDCQYLNF
                     SSNDYLGLSHHPQIIRAWQQGADQFGVGSGGSGHVSGYSVAHQVLEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSPLRRFAHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLEEIQQVTQQHDGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSNTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSDEGDARREKLAALITRFRAGVQDLPFTLAGSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            9774..10529
                     /gene="bioC"
                     /locus_tag="ACHKFK_001965"
     CDS             9774..10529
                     /gene="bioC"
                     /locus_tag="ACHKFK_001965"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO9052312.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     RVLDAGCGPGWMSRRWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNAVHYQHYIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            10522..11199
                     /gene="bioD"
                     /locus_tag="ACHKFK_001966"
     CDS             10522..11199
                     /gene="bioD"
                     /locus_tag="ACHKFK_001966"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO9052313.1"
                     /translation="MSKCYFVTGTDTEVGKTVASCALLQAAKAAGYRTAGYKPVASGS
                     EKTPKGLRNSDALALQRNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIEALVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAILHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALM"
     gene            11778..13799
                     /gene="uvrB"
                     /locus_tag="ACHKFK_001967"
     CDS             11778..13799
                     /gene="uvrB"
                     /locus_tag="ACHKFK_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO9052314.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(13837..14745)
                     /gene="yvcK"
                     /locus_tag="ACHKFK_001968"
     CDS             complement(13837..14745)
                     /gene="yvcK"
                     /locus_tag="ACHKFK_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO9052315.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDAHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAISEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKEDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHSALEKALQALG"
     regulatory      15010..15154
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            15142..16131
                     /gene="moaA"
                     /locus_tag="ACHKFK_001969"
     CDS             15142..16131
                     /gene="moaA"
                     /locus_tag="ACHKFK_001969"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO9052316.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGIELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            16153..16665
                     /gene="moaB"
                     /locus_tag="ACHKFK_001970"
     CDS             16153..16665
                     /gene="moaB"
                     /locus_tag="ACHKFK_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO9052317.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLVFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            16668..17153
                     /gene="moaC"
                     /locus_tag="ACHKFK_001971"
     CDS             16668..17153
                     /gene="moaC"
                     /locus_tag="ACHKFK_001971"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO9052318.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            17146..17391
                     /gene="moaD"
                     /locus_tag="ACHKFK_001972"
     CDS             17146..17391
                     /gene="moaD"
                     /locus_tag="ACHKFK_001972"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO9052319.1"
                     /translation="MINVLFFAQVRELVGTDATEVAADFPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHSLTDGDEVAFFPPVTGG"
     gene            17393..17845
                     /gene="moaE"
                     /locus_tag="ACHKFK_001973"
     CDS             17393..17845
                     /gene="moaE"
                     /locus_tag="ACHKFK_001973"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO9052320.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            17982..18686
                     /gene="ybhL"
                     /locus_tag="ACHKFK_001974"
     CDS             17982..18686
                     /gene="ybhL"
                     /locus_tag="ACHKFK_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9052321.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            18891..19604
                     /gene="ybhM"
                     /locus_tag="ACHKFK_001975"
     CDS             18891..19604
                     /gene="ybhM"
                     /locus_tag="ACHKFK_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO9052322.1"
                     /translation="MESYSQNSNKLDFQHEARILNSIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPQMYVAIGLLLLSMYGLSKDINKINTAIAGVIYLFLISLVAIVVASLAP
                     VSAIIVVFSTAGSMFLISMLAGLLFKFDPGSHRFIIMMTLTGLALVIIVNATLMSERP
                     IWVISCLMIVLWSGIISHGRNKLLELTGKSHSEELWSPVRCAFVGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(19640..20596)
                     /gene="ybhN"
                     /locus_tag="ACHKFK_001976"
     CDS             complement(19640..20596)
                     /gene="ybhN"
                     /locus_tag="ACHKFK_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO9052323.1"
                     /translation="MSKSHPRWRLAKKLLTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGVIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWEFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(20596..21837)
                     /gene="clsB"
                     /locus_tag="ACHKFK_001977"
     CDS             complement(20596..21837)
                     /gene="clsB"
                     /locus_tag="ACHKFK_001977"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO9052324.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVGP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(21834..22595)
                     /gene="ybhP"
                     /locus_tag="ACHKFK_001978"
     CDS             complement(21834..22595)
                     /gene="ybhP"
                     /locus_tag="ACHKFK_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9052325.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRTHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            22728..23138
                     /gene="ybhQ"
                     /locus_tag="ACHKFK_001979"
     CDS             22728..23138
                     /gene="ybhQ"
                     /locus_tag="ACHKFK_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO9052326.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(23100..24206)
                     /gene="ybhR"
                     /locus_tag="ACHKFK_001980"
     CDS             complement(23100..24206)
                     /gene="ybhR"
                     /locus_tag="ACHKFK_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9052327.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVVYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPMWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(24217..25350)
                     /gene="ybhS"
                     /locus_tag="ACHKFK_001981"
     CDS             complement(24217..25350)
                     /gene="ybhS"
                     /locus_tag="ACHKFK_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9052328.1"
                     /translation="MSNPILSWRRVRALCFKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(25343..27079)
                     /gene="ybhF"
                     /locus_tag="ACHKFK_001982"
     CDS             complement(25343..27079)
                     /gene="ybhF"
                     /locus_tag="ACHKFK_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9052329.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPTALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEIDINETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     NSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHGNE"
     gene            complement(27072..28067)
                     /gene="hlyD"
                     /locus_tag="ACHKFK_001983"
     CDS             complement(27072..28067)
                     /gene="hlyD"
                     /locus_tag="ACHKFK_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO9052330.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQSAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPNKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(28070..28741)
                     /gene="cecR"
                     /locus_tag="ACHKFK_001984"
     CDS             complement(28070..28741)
                     /gene="cecR"
                     /locus_tag="ACHKFK_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO9052331.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLASAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     SDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            28970..30331
                     /gene="rhlE"
                     /locus_tag="ACHKFK_001985"
     CDS             28970..30331
                     /gene="rhlE"
                     /locus_tag="ACHKFK_001985"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO9052332.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGRGQGGGRGQQQPRRAESGAKSGNAKPAEKPSRRLGDAKPAGEQQRRRRPRKPA
                     AAQ"
     gene            complement(30564..30764)
                     /locus_tag="ACHKFK_001986"
                     /pseudo
     CDS             complement(30564..30764)
                     /locus_tag="ACHKFK_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
     gene            30868..33018
                     /gene="dinG"
                     /locus_tag="ACHKFK_001987"
     CDS             30868..33018
                     /gene="dinG"
                     /locus_tag="ACHKFK_001987"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO9052333.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            33046..34008
                     /gene="ybiB"
                     /locus_tag="ACHKFK_001988"
     CDS             33046..34008
                     /gene="ybiB"
                     /locus_tag="ACHKFK_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO9052334.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLMATGEAATISDGLARVNQAF"
     gene            34149..35234
                     /gene="hcxB"
                     /locus_tag="ACHKFK_001989"
     CDS             34149..35234
                     /gene="hcxB"
                     /locus_tag="ACHKFK_001989"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO9052335.1"
                     /translation="MVSGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKVVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQELGIPLDAGS
                     WQAICDAARQVGMPEETLQAFCQQLAS"
     gene            35301..35360
                     /gene="ybiE"
                     /locus_tag="ACHKFK_001990"
     CDS             35301..35360
                     /gene="ybiE"
                     /locus_tag="ACHKFK_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO9052336.1"
                     /translation="MRLSANRQGNTCAERSVNA"
     gene            complement(35463..35723)
                     /gene="ybiJ"
                     /locus_tag="ACHKFK_001991"
     CDS             complement(35463..35723)
                     /gene="ybiJ"
                     /locus_tag="ACHKFK_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO9052337.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(35988..36254)
                     /gene="ybiI"
                     /locus_tag="ACHKFK_001992"
     CDS             complement(35988..36254)
                     /gene="ybiI"
                     /locus_tag="ACHKFK_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO9052338.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(36328..37005)
                     /gene="ybiX"
                     /locus_tag="ACHKFK_001993"
     CDS             complement(36328..37005)
                     /gene="ybiX"
                     /locus_tag="ACHKFK_001993"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO9052339.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPESYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(37047..39329)
                     /gene="fiu"
                     /locus_tag="ACHKFK_001994"
     CDS             complement(37047..39329)
                     /gene="fiu"
                     /locus_tag="ACHKFK_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO9052340.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLITAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGAIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQMIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(39594..>39680)
                     /locus_tag="ACHKFK_001995"
     CDS             complement(39594..>39680)
                     /locus_tag="ACHKFK_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgH/BhsA/McbA-like domain containing protein"
                     /protein_id="HFO9052341.1"
                     /translation="AREQGAKGYVINSAGGNDQMFGTAIIYK"
BASE COUNT         9384 a        10288 c        10696 g         9312 t
ORIGIN      
        1 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa
       61 tcagtaagtt ggcagcatca cccgcttctg gtagtgccta tatcgttgcc atgttaccgg
      121 tatttgccat gctggcgaat gtctccggcg caccgttaat gttaaccgcg ctggcactgt
      181 tgttctctaa ctcctatggc ggcatggtta ctcactatgg cggcgcggca ggtccggtca
      241 tctttggcgt gggttacaac gatattaaat cctggtggtt ggtcggtgcg gtactgacga
      301 tattaacctt cctggtgcat atcaccctcg gcgtgtggtg gtggaatatg ctgatcggct
      361 ggaacatgct gtaaatatac tcgtcatact tcaagttgca tgtgctgcgt ctgcgttcgc
      421 tcaccccagt cacttactta tgtaagctcc tggggattca ctcgcttgtc gccttcctgc
      481 aactcgaatt atttagagta tatccattta ttatctttct gcgcaattca cggtgcgcag
      541 atatctggag catttgatga tcaagttatc tgaaaaaggc gtgtttctcg ccagcaataa
      601 cgaaataatt gccgaagaac atttcaccgg cgaaataaaa aaagaagaag caaaaaaagg
      661 cactattgcc tggtctattc tctcttctca taatacgtcc ggaaatatgg ataaacttaa
      721 aattaagttt gattcattag cctctcacga tattaccttt gttggtattg tacagaccgc
      781 taaagcgtcc ggtatggaac gtttcccgct gccgtatgtg ctgaccaact gccataactc
      841 actctgtgct gtgggcggca ccattaacgg tgatgaccat gtttttggtt tatcggcggc
      901 ccagcgttat ggcggtattt ttgtgccacc gcatattgcg gtcatccatc aatatatgcg
      961 tgaaatgatg gcgggcggcg gcaaaatgat cctcgggtca gacagccata cccgttacgg
     1021 tgcgttaggg acaatggcgg ttggcgaggg cggcggcgag ttggtaaaac agctgcttaa
     1081 tgacacctgg gatatcgact atccgggcgt ggttgcggtg catctgaccg gaaaacctgc
     1141 gccgtatgtg gggccgcagg atgtggcgct ggctatcatc ggtgccgtgt tcaaaaacgg
     1201 ttacgtcaaa aacaaagtga tggagttcgt aggtcccggt gttgcagcgc tctctaccga
     1261 tttccgtaac agcgttgacg tgatgaccac tgaaacgacc tgtttaagtt ctgtctggca
     1321 aaccgatgaa gaagtccata actggctggc gctgcacggt cgcggccagg attactgcca
     1381 acttaaccct caaccaatgg cgtactacga tggcagcatc agcgttgatt taagcgccat
     1441 caaaccaatg attgcgctgc cattccaccc gagcaacgtg tatgaaatcg acacgctgaa
     1501 ccagaatctg accgacattc tgcgtgagat tgaaattgag tccgaacgcg tggcgcacgg
     1561 taaagccaaa ctctcgctgc tggataaagt ggaaaatggt cgcctgaaag tgcagcaggg
     1621 gattatcgcg ggctgttctg gcggtaacta cgaaaacgtc atcgcggcgg cgaatgcgct
     1681 gcgcggtcaa tcctgcggca acgatacctt ctcgttggcg gtttacccgt catcacagcc
     1741 ggtgtttatg gatctcgcca aaaaaggtgt ggtagcagat ttgattggcg caggcgcaat
     1801 catcagaacc gcgttctgcg gcccatgctt tggtgcggac gatacgccaa tcaacaacgg
     1861 tttgagtatt cgccacacca cgcgtaactt cccgaaccgc gaaggctcta agccagctaa
     1921 tgggcagatg tcagcggtgg cgttgatgga cgctcgttct atcgctgcga ctgcggcaaa
     1981 cggtggctat ttaacctctg ccagcgaact cgattgctgg gacaacgtgc cggagtacgc
     2041 cttcgatgta acgccgtata aaaaccgtgt ttatcagggc tttgtgaaag gggcgaccca
     2101 gcaaccgctg atttacggac cgaacattaa agactggcca gaattgggtg cgctgactga
     2161 caatatcgtc ctcaaagtgt gctcgaagat cctcgacgaa gtgaccacca ccgacgaact
     2221 gattccttcc ggtgaaacct cttcatatcg ttcaaatccg attggtctgg cggagtttac
     2281 tctgtcacgc cgcgatccag gttatgttgg cagaagtaaa gcgactgccg agctggaaaa
     2341 tcagcgtctg gcgggaaatg tcagcgagat ggcagatgtg tttgcgcgca ttaagcagat
     2401 tgctggtcag gaacatgttg atccgctgca aactgaaatt ggcagcatgg tttatgcggt
     2461 gaaaccaggc gatggttctg cgcgtgaaca ggcggcgagt tgtcagcgtg tgattggcgg
     2521 tctggcgaat attgccgagg agtacgcgac taaacgctat cgttctaacg tcatcaactg
     2581 ggggatgtta ccgctgcaga tggcggaagt accgaacttt gacgtaggtg attacattta
     2641 tatccctggc attaaagcgg cgctggataa tcctagaacg acgtttaaag gttatgtgat
     2701 ccatgaagat gcaccggtaa cggaaatcac gctgtatatg gaaagtctga ctgccgaaga
     2761 acgcgagatt atcaaggcgg gtagtttgat taacttcaat aaaaaccgtc agatgtaaaa
     2821 agcgccatgt gaatgtaggt cgcatccggc acttattgtc ggatgcgatg ctggcgcatc
     2881 ttatccgacc gacaaatcgt atcgaactgt aggccggata aggcgtttac gccgcatccg
     2941 gcacatagtt aacagctcct acttcttcgc ctctgcaacc actttgctac ccacgccgcg
     3001 gttattgtat tcccacatgc ggttgtagtt agtgtcattc agattgcgct gtacttcgtc
     3061 gttatcatca acgctgccgg tgttacccgc aaacggacga ttagagatca ccgcatcagc
     3121 ccacggtttg gctgtgttaa aaccttcgtt gatggcgcta tcacggatca ctacctgacc
     3181 gttggtattg gcatcaacat ccagcgagcg gcccagttgc gccacgccat caccggaagc
     3241 attgaaacgg ctgtttacgg cgaggaaacc gtagtaaatg ttggacagcg tagccggtgc
     3301 aaacacatac gcttcttgct gggtacggga gttcaccacg cggaattcgg tgttatcgaa
     3361 caccactgcg ccgcgaccag aaacgatatc cacatcccct tcaatatagc tgttggtcac
     3421 cagcgtacgc ggctgacgat tcgtttccag acggttctgc acaccgctgt tggtgacaaa
     3481 gaaggtgttc tgacgaccga gaatgttgac gttattgatc tgcactttgt cgccatcagt
     3541 acgcagtgcc accgccggat ggttacccgc atctacgcta tcgcccagcg tgttttcgat
     3601 ggtcaggttt tgcagttgca ggccattgtt ttgtgaccag aagaccgcag agcagagaac
     3661 accgatactg tcgctgcgtt tactctggca gctatcgtac atataccacg ccggtttacc
     3721 tggcatatat ttgccgcgcg ggttgacgtc gtgacgccag tcggcagggc tcatgccacc
     3781 atcaagggaa agcccaatct tcacatcaat cggtttttca cctgtaccgt acagagtaat
     3841 tccacccgga gcggcaggga catacaccgt tccctgatac tcaccaggca tcacggcaat
     3901 atactggcgc ttgttggtac gcttgataat tgccgcatct accgccgcct gaatcgtggt
     3961 atgcgttaca ccttgagtac ccgccgggcc gacaacaaag tcaggttgcg caggcagggt
     4021 aatcggggaa ggattccatg ctgccgcacc tggtgtcagg gatgcaaaat agtgttgagc
     4081 atcgaaattc tgcgcttctt ttgccgacag aatcgggcga gaagaggtac caggcgcggt
     4141 ttgatcagaa ggacgttgat cgggcggtgt tgagctacag gcggtcagcg tcacgccaaa
     4201 agccaatgcc agcgccagac gggaaactga aaatgtgttc acaggttgct ccgggctatg
     4261 aaatagaaaa atgaatccgt tgaagcctgc ttttttatac taacttgagc gaaacgggaa
     4321 ggtaaaaaga caaaaagttg tttttaatac ctttaagtga taccagatgg cattgcgcca
     4381 tctgacagag tgattaacta aacatcgcag taatcgaggc actcgccaga gagtgaaaat
     4441 gaacgttaaa cccgaccatc gcgccgctgg caccttcatc gacatcaata cgttctacat
     4501 ccagcgcgtg aacggtaaaa atgtagcgat gggtttcgcc tttcggcggc gctgcgccat
     4561 cgtatccggt tttaccaaag tcggtacgcg tctgcaaaac gccgtctggc attgctacca
     4621 gaccagagcc aaacccttgc ggtaatacgc gggtatcagc gggtaaatta acaactaccc
     4681 agtgccacca gccggagccg gttggcgcat ccgggtcata gcaggtgaca acaaaacttt
     4741 tcgttcccac aggaacatca tcccacgcca gatgcggtga aatattatcg ccatcgtaac
     4801 ccatgccgtt aaagacatga cgatgcggca gcttatcgcc atcgcgcaga tcgttactga
     4861 tgagtttcat tagaatgcct ccgggaaacc tcggccttca gaccggggag gaaaggaggc
     4921 ggttttccga ctaactgtac tttgcataat cacattttcc tctttagtat gtgaacacat
     4981 gaaacgcgca tataaatacc ggttttaccc gacaactgag caggctgagc ttttagctca
     5041 gacgtttggc tgtgtgcgct tcgtctacaa ttccatcctt cgttggcgta ccgatgcgta
     5101 ctacgagcga aaagaaaaga tcggttatct acaggccaac gctcgcctta cggcgctcaa
     5161 aaaagagcct gaatacatat ggctgaatga tgtttcctgc gttcccctcc agcagtcgtt
     5221 gcgccaccaa caagccgcct ttgctaactt ctttgccgga cgagctgcat atccggcttt
     5281 caaaagcaaa cggcacaaac aggtggctga gttcactgcc agcgcgttta aacaccgtga
     5341 cggcgagttg tatatagcaa agagcaagtc gccgctggat gttcgctgga gtcgagaatt
     5401 accatctgcg ccgtcaaccg ttaccatttc cagagatagc gctggcaggt actttgtttc
     5461 ctgcctgtgt gagtttgaac ctgtatcaat gcctgttacc gctaaaacgg tcggcattga
     5521 tgtgggctta aaagatttat tcgtcaccga taccggattc aaaaccgaca atccccgcca
     5581 caccgctaaa tatgcgaagc gattaacgct gctacagcga cgtttaagca ggaagcaaaa
     5641 aggctcaaga aaccgtatta aagcccgctt aaaggtcgcc cgactccatg cgaaaatcgc
     5701 cgattgccgg atggacaatc tgcacaagtt gtcccgcaaa ctgattaacg aaaaccaagt
     5761 tgtttgcgtc gaatccctca aggtgaaaaa catgatccgc aacccgaagc tgtctaaagc
     5821 aatagctgac gcaggctgga gcgaacttgt tcgccagctc cagtacaaag gcaaatgggc
     5881 cgggcggtca gtggtcgcca ttgaccagta tttaccgtcc tcaaaatgct gtagttgctg
     5941 cggtttcacc atgcaaaaaa tgcctcttaa tgttcgtaaa tggcactgcc ctgaatgcgg
     6001 cgcagaccat gatcgcgaca ttaacgcggc acgtaatatt aaagctgccg ggctggcagt
     6061 gttagcccac ggagagcctg taaaccctga atcgcagcac gcggcttagg ttcggctcgt
     6121 tgaagtggga atccccgtca ttcagggcgg ggagccgtca agagccctcc tttcttgttt
     6181 gcagaaagtg tagccagaaa ccctcacgct gacttcccgt tattggcaaa aaaatgtttc
     6241 atcctgcacc gcacggttaa ccgctgcggt cagacgctgc aactgttgcg ggagaataat
     6301 atagggcggc atcaggtaaa tcagtttgcc aaaaggccgg atccagacac cctgttcgac
     6361 aaagaatttt tgcagcgctg ccatattcac cggacgagtg gtttcgacca cgccgattgc
     6421 ccccagtacg cgcacatcgg caaccatttc ggcgtcacag gcgggggcaa gttgctcgcg
     6481 tagctgcgct tcaatagccg ccacctgatg ctgccagtcg ccagattcaa gaatcgccag
     6541 gctggcgttt gctgctgcgc aggccagcgg attgcccata aaagttggcc catgcataaa
     6601 gcagccggct tcgccgttac tgatggtttc cgcaacctcg cgcgtggtga gcgtggcgga
     6661 aagggtcatt gtgccgccgg ttaaggcttt accgagacac aaaatatccg gcgcgatttc
     6721 tgcatgttca caggcaaaca gtttgccggt acgaccaaat ccggtggcga tctcgtcggc
     6781 aatcagcaag ataccgtcgc gatcgcacat tttgcggatt cgttttaacc attccggatg
     6841 gtacatgcgc atcccgcctg cgccctggac aatcggttca atgatcaccg ccgcgatttc
     6901 atgacgatgc gctgccatca ggcgggcaaa gcccaccata tcgcgctcat cccattcgcc
     6961 atccatgcgg ctttgcgggg cgggagcaaa caggttttct ggcagatagc ctttccacag
     7021 gctgtgcatt gagttgtccg gatcgcacac cgacatcgcg ccaaaggtat cgccatgata
     7081 accattgcgg aaggttagaa aacgctggcg cgcttcgcct ttggcctgcc agtactgcaa
     7141 cgccattttc atcgccactt ctaccgctac ggagccggag tccgcgagaa aaacgcactc
     7201 cagcggttgc ggcgtcattg ccaccaattt gcggcacagc tcaatggcgg gcgcatgggt
     7261 gataccgcca aacatcacat gcgacatagc atcaatttgc gacttcatcg ccgcattaag
     7321 ctgcgggtga ttgtaaccgt ggatcgccgc ccaccaggac gacataccgt caaccaggcg
     7381 tctgccgtca gacaaaatta gctcgcaacc ttcggcgctt gccaccggat aaaccggcag
     7441 cggggaggtc atggatgtgt atgggtgcca gatatggcgt tggtcaaagg taagatcgtc
     7501 cgttgtcata atcgacttgt aaaccaaatt gaaaagattt aggtttacga gtctacaccg
     7561 aattaacaac aaaaaacacg ttttggagaa gccccatggc tcaccgccca cgctggacat
     7621 tgtcgcaagt cacagaatta tttgaaaaac cgttgctgga tctgctgttt gaagcgcagc
     7681 aggtgcatcg tcagcatttc gatcctcgtc aggtgcaggt cagcactttg ctgtcgatta
     7741 agaccggagc ttgtccggaa gattgcaaat attgcccgca aagctcgcgc tacaaaaccg
     7801 ggctggaagc cgagcggttg atggaagttg aacaggtgct ggagtcggcg cgcaaagcga
     7861 aagcggcggg atcgacgcgt ttctgtatgg gcgcggcgtg gaagaatccc cacgaacgcg
     7921 atatgccgta cctggaacaa atggtgcagg gggtaaaagc gatgggtctg gaggcgtgta
     7981 tgacgctggg cacgttgagt gaatctcaag cgcagcgcct ggcgaatgcc gggctggatt
     8041 actataacca caacctcgac acctcgccgg agttttacgg caatatcatc accacccgca
     8101 cctatcagga acgtctcgat acgctggaaa aagtgcgaga agctgggatt aaagtctgtt
     8161 caggtggcat tgtgggctta ggcgaaacgg tgaaagatcg cgccggatta ttgctgcaac
     8221 tggcaaactt gccgacgccg ccggaaagcg tgccaatcaa catgctggtg aaggtgaaag
     8281 gcacgccgct ggcggataac gacgatgtcg atgcctttga ttttatccgt accattgccg
     8341 tagcgcggat catgatgccg acctcttacg tacgcctttc tgccggacgc gagcagatga
     8401 acgaacagac tcaggcgatg tgctttatgg ctggcgcaaa ctcgattttc tacggttgca
     8461 aactgctgac tacgccgaat ccggaagaag ataaagacct gcaactgttc cgcaaactgg
     8521 ggctaaatcc gcagcaaact gccgtgctgg cgggcgataa cgaacaacag cagcgtctgg
     8581 agcaggcgct gatgaccccg gacactgacg aatattacaa cgcggcagca ctatgatctg
     8641 gcaggagaaa atcgacgcgg cgcttgatgc gcggcgtgtt gctgatgcct tgcgtcgccg
     8701 ttatccggtg gcacaaggag ccggacgctg gctggtggca gatgattgcc agtatctgaa
     8761 cttttccagt aatgattatt taggtttaag ccatcatccg caaattatcc gcgcctggca
     8821 gcagggcgcg gatcaatttg gtgtcggtag cggcggttcc ggtcacgtca gcggttatag
     8881 cgtggcgcac caggtgctgg aagaagaact ggccgaatgg ctgggctatt cgcgggcgct
     8941 gctgtttatt tctggttttg ccgctaatca ggctgtcatt gccgcgatga tggcgaaaga
     9001 ggaccgtatt gttgccgatc ggcttagcca tgcctcattg ctggaggctg caagtttaag
     9061 cccgtcgccg cttcgccgtt ttgctcataa cgatgtcact catctggcgc gactgcttgc
     9121 ttccccctgt ccggggcagc aactggtagt gacagaaggt gtgttcagca tggacggcga
     9181 tagtgcgcca ctggaggaaa tccagcaggt aacgcaacag cacgatggct ggttgatggt
     9241 cgatgacgcc cacggcacgg gcgttatcgg ggagcagggg cgtggcagct gctggctgca
     9301 aaaggtaaaa ccagaattgc tggtggtgac gtttggcaaa ggatttggcg tcagcggggc
     9361 agcggtgctt tgctccaata cggtggcgga ttatctgctg caattcgctc gccatcttat
     9421 ctacagcacc agtatgccgc ccgctcaggc gcaggcatta cgtgcgtcgc tggcggtcat
     9481 tcgcagtgat gagggggatg cacggcgcga aaaactggcg gcactcatta cgcgttttcg
     9541 tgccggagtg caggatttgc cgtttacgct tgctggctca tgcagcgcca tccagccatt
     9601 gatcgtcggt gataacagcc gtgcgttaca actggcagaa aaactgcgcc agcaaggctg
     9661 ctgggtcacg gcgattcgcc cgccaaccgt acccgctggt actgcgcgac tgcgcttaac
     9721 gctaaccgca gcgcatgaaa tgcaggatat cgaccgtctg ctggaggtgc tgcatggcaa
     9781 cggttaataa acaagccatt gctgcggcat ttggacgggc agccgcacac tatgagcaac
     9841 acgccgatct acagcgccag agtgctgacg ccttactggc aatgctccca cagcgtaaat
     9901 acacccgcgt actggatgcg ggttgtggac caggctggat gagccgccgc tggcgagagc
     9961 gtcacgcgca ggtgactgcg ttagatctct ctccgccaat gcttgttcag gcacgccaga
    10021 aggatgccgc agaccattat ctggcgggag atatcgaatc cctgccgtta gcgactgcga
    10081 cgttcgatct tgcatggagc aatctcgcgg tgcagtggtg cggtaattta tccacggcac
    10141 tccgcgagct gtatcgggtg gtgcgccccg gaggcgtggt cgcgtttacc acgctggtgc
    10201 agggatcgtt acccgaactg catcaggcgt ggcaggcggt ggacgagcgt ccgcatgcta
    10261 atcgcttttt accgccagat gaaatcgaac agtcgctaaa cgccgtgcat tatcaacatt
    10321 atattcagcc catcacgctg tggtttgatg atgcgctcag tgccatgcgt tcgctgaaag
    10381 gcatcggtgc cacgcatctt catgaagggc gtgacccgcg aatattaacg cgttcgcagt
    10441 tgcagcgatt gcaactggcc tggccgcaac aacaggggcg atatcctctg acgtatcatc
    10501 tttttttggg agtgattgct cgtgagtaaa tgttattttg tcaccggaac ggataccgaa
    10561 gtggggaaaa ctgtcgccag ctgcgcgctt ttacaagccg caaaggcagc aggctaccgg
    10621 acggcaggtt acaaaccggt ggcctctggc agcgaaaaga ccccgaaagg cttacgtaat
    10681 agtgacgcgc tggcgttaca gcgcaacagt agcctgcagc tggattacgc aacggtaaat
    10741 ccttacacct tcgcagaacc cacctcgccg catatcatca gcgcgcaaga gggcaggccg
    10801 atagaagcat tggtaatgag cgccggatta cgcgcgcttg aacaacaggc tgactgggtg
    10861 ttagtggaag gtgctggcgg ctggtttacg ccgctttctg acactttcac ttttgcggat
    10921 tgggtaacac aggaacaact gccagtgata ctggttgtgg gcgtgaaact cggctgtatt
    10981 aatcacgcga tgttgactgc gcaggcaata ctacacgccg gactgactct ggcggggtgg
    11041 gtggcgaacg atgtcacgcc tccgggaaaa cgtcacgctg aatatatgac cacgctcacc
    11101 cgcatgattc ccgcgccgct gctgggcgaa attccctggc ttgcagaaaa tccagaaaat
    11161 gcggcaaccg gaaagtacat caatcttgcc ttaatgtagt catcctgtgc ttggttaaat
    11221 tgtgagcgag atcgcgtctc cgattgactg caatttaacc aattaaattc taaaaaaatc
    11281 acgaaaaaaa ttttacctcc gcatcatgcg gtgaatgtgg gaattgccca ggcggcgggg
    11341 gataggggct gcagacagtt atccactatt cctgtggata accatgtgta ttagagttag
    11401 aaaacacgag gcaagcgaga gaatacgcgg cttgcacgcg aattggcgtt aaagacggct
    11461 caaagaaata tcttttattt tttaacaggt tagataattg caatggcagt cactgaacag
    11521 gcatctcttg ccataaaact gtcatcaatc atcttgacaa atgttaaaaa agccgttgct
    11581 ttggggataa cccggtaagg ccggagtttt atctcgccgc agagtaaatt ttgctcatga
    11641 ttgacagcgg agtttacgct gtaacagaaa tattatggtg atgaactgtt tttttatcca
    11701 gtataatttg ttggcataat taagtacgac gagtaaaatt acatacctgc ccgcccaact
    11761 ccttcaggta gcgactcatg agtaaaccgt tcaaactgaa ttccgctttt aaaccttctg
    11821 gcgatcagcc ggaggcgatt cgacgtctcg aagaggggct ggaagatggc ctggcgcacc
    11881 agacgttact tggcgtgact ggctccggga aaaccttcac cattgccaat gtcattgctg
    11941 accttcagcg cccaaccatg gtacttgcgc ccaacaaaac gctggcggcc cagctgtatg
    12001 gcgaaatgaa agaattcttc ccggaaaacg cggtggaata tttcgtctcc tactacgact
    12061 actatcagcc ggaggcctat gtaccgagtt ccgacacctt cattgagaaa gatgcctcgg
    12121 taaacgaaca tatcgaacag atgcgtttgt ccgccaccaa agcgatgctg gagcggcgtg
    12181 atgtggttgt ggtggcgtct gtttccgcga tttatggtct gggcgatcct gatttatatc
    12241 tcaagatgat gctccatctc acggtcggta tgattatcga tcagcgcgcg atcctgcgcc
    12301 gactggcgga gctgcaatat gctcgtaatg atcaagcatt ccagcgtggt actttccgtg
    12361 ttcgtggcga ggtgattgat atcttcccgg cagaatcgga tgacattgca cttcgcgtgg
    12421 agctgtttga cgaggaggtg gaacgattgt cgttatttga cccgctgacc gggcagattg
    12481 tttccactat tccacgtttt accatctacc cgaaaacgca ctacgtcacg ccacgcgagc
    12541 gcatcgtcca ggcgatggag gagatcaaag aagagctggc cgccagacgt aaagtgctat
    12601 tggaaaacaa caaactgctg gaagagcagc ggctgaccca gcgtacccag tttgatctgg
    12661 agatgatgaa cgagctaggc tactgttcgg ggattgagaa ctactcgcgc ttcctctccg
    12721 gtcgtggacc gggtgagcca ccgccgacgc tgtttgatta cctgcctgct gatgggctgc
    12781 tggtggtcga tgaatctcac gtcaccattc cacaaattgg cggtatgtat cgcggtgacc
    12841 gggcgcgtaa agagacactg gtggagtacg gcttccgcct gccatcagcg ctggataacc
    12901 gtccgctgaa atttgaagag ttcgaagcat tagcgccgca aaccatctat gtttcggcga
    12961 cgccgggtaa ttacgagctg gaaaaatccg gtggcgatgt ggtggatcag gtggtgcgtc
    13021 caacaggctt actcgacccg attatcgaag tgcggccggt agcgacacag gtggatgatc
    13081 ttctatcgga gattcgtcag cgagcggcaa ttaacgaacg cgtactggtt acaactctga
    13141 ccaagcggat ggcggaagat ctcactgaat atctcgaaga acacggtgag cgcgtgcgtt
    13201 atcttcactc agatatcgac accgtcgaac gtatggagat tatccgcgac ttgcgtctgg
    13261 gtgagttcga cgtattggta gggatcaact tactgcgcga aggtctggat atgccggaag
    13321 tttcgctggt ggcgatcctc gacgctgaca aagaaggctt cctgcgttcc gaacgttcgt
    13381 tgatccagac cattggtcgt gcggcacgta acgttaacgg taaagcgatt ctctacggcg
    13441 ataagatcac cccatcaatg gcgaaagcga ttggcgaaac cgaacgtcgc cgcgagaaac
    13501 agcagaagta caacgaagaa cacggcatta cgccgcaagg cttgaacaag aaagtggtcg
    13561 atatcctggc gctggggcag aacattgcca aaaccaaagc gaagggcaga ggaaaatcgc
    13621 gcccgattgt tgaaccggat aatgtgccga tggatatgtc gcctaaagcg ttacagcaga
    13681 agatccatga actggaaggg ttgatgatgc aacacgcgca gaatctggag ttcgaagaag
    13741 cggcgcaaat tcgtgaccag ttgcatcagt tgcgtgagct gtttattgcc gcgtcgtgac
    13801 aagatgacga agccggaata ttcttccggc ttcgtgttaa cctaaagcct gtaatgcctt
    13861 ttccagcgcg ctatgcaaca actggcggtc atgacgatac ggaatatcgc tggcctccag
    13921 cacttcctga atcacaatcc gctctttcac cgccgagaca tcctcttttg gcccgacgat
    13981 gaccgcatca attacctttt taccaacata ctgctccata attgccagct tgctttccag
    14041 cttcagatta gccgcaggta aactcaactc acgccccaga ttgccgatat agaccatcgg
    14101 cgctggcgtg cggcgtaatg cctgtgcgat ttccttcagc agcagaattg gcatcaggct
    14161 ggtgtaaaaa ctgccaggcc caataatgat gagatccgct tcactgatag cgtgaaccgc
    14221 ctcacgcgtt gcgggtacat tcggcgttaa caataactct tgaatcggcg tagttaactg
    14281 gtcgatattg acctcgccgt atacttcatg cccctgatcg tcaatcgcca tcagatcaac
    14341 aggatgctcc gacatcggaa tcaaatgtgc gtccactttc agcagatttc gaatcagatt
    14401 gatcgcttca agaggtcgca cgctaaggtg atccagtgcc tttaacatca ggtttccaag
    14461 gttgtgcccg gaaagttcgc cattaccgcc gaaacgatat tcaaacattg cggaagcgac
    14521 gctgggttca gttatcagct gattgaggca gttgcgcata tcgccccagg caatgccgcc
    14581 ttctgagcgg cgaatacgcc ccgtcgagcc accgttatcg gtggtggtga cgatacccgt
    14641 taaacgagaa cccaaagacg aaagtgatga gagaacgcgt cccagtccat gccctccgcc
    14701 gagagcaacg acacgatcaa gatcagccag cgtacgattg cgcatatagt ttcctggagt
    14761 cagattatcc gcgctacagt agcgcaaatt gcgaggaaac agcaattaac ctgccaatat
    14821 tgaggataca aactattttc ttctacctct aaaggacgat gcacgctatg cctccctgat
    14881 gatgtatatc aaagtaaaac cgccattttc ccttattctg tagcgaaata gcacgatcat
    14941 gacgctatat acatgattac atagcgaaag tgtggatggt aaaaatctca tttacacgct
    15001 agtatcggct taaccactaa acactctagc ctctgcacct gggtcaactg atacggtgct
    15061 ttggccgtga caatgctcgt aaagattgcc accagggcga aggaagaaat gacttcgcct
    15121 cccgtatctg gaaaggtgta catggcttca caactgactg atgcatttgc gcgtaagttt
    15181 tactacttgc gcctgtcgat tactgatgtg tgtaactttc gttgcaccta ctgcctgccg
    15241 gatggttaca agccgagcgg cgtcaccaat aaaggctttc tgaccgtcga tgaaattcgc
    15301 cgggttacgc gcgccttcgc cagtttgggc accgaaaaag tccgtctgac aggtggtgag
    15361 ccatctttac gccgcgactt taccgatatc atcgccgctg tgcgagaaaa cgacgctatt
    15421 cgccagatag cggtcaccac caatggttac cgtctggagc gcgatgtggc gaactggcgc
    15481 gatgctggac ttactggcat caacgtcagc gtcgacagtc tggacgctcg ccagtttcat
    15541 gccattaccg ggcaggataa attcaaccag gtcatggcag gaatcgatgc tgcatttgag
    15601 gccggttttg agaaggtcaa agtcaatacc gtgctgatgc gtgatgttaa tcatcaccag
    15661 ctcgacacct ttctgaactg gatccagcat cgtcccatcc agctgcgttt catcgaactg
    15721 atggaaacgg gcgagggcat cgagctcttc cgtaagcatc acatctctgg tcaggttctg
    15781 cgtgacgagc tactgcgtcg cggctggatc caccaattac gtcaacgcag cgacggtccc
    15841 gcgcaagtct tttgtcatcc ggattacgcc ggagagattg gccttatcat gccgtatgaa
    15901 aaagacttct gcgccacttg caaccgcctg cgcgtttcct ccattggtaa actccatctc
    15961 tgcctgtttg gtgaaggcgg cgttaacctg cgcgatctgc tggaagacga tacccagcaa
    16021 caggcgctgg aagcgcgtat ttcagcggcg ctgcgggaga aaaaacagac ccatttcctg
    16081 catcaaaata acaccggtat tacgcaaaac ttatcgtaca ttggtggcta aaacgtcaaa
    16141 aggagagatc agatgagtca ggtaagcact gaatttatcc cgacccgtat tgctattctt
    16201 acggtttcta atcgtcgcgg tgaagaagac gatacctccg gtcactatct gcgcgattcg
    16261 gcgcaagaag cgggccatca cgttgtcgat aaagccattg tgaaagaaaa ccgctacgct
    16321 attcgcgctc aggtatctgc gtggatcgcc agcgacgatg tacaagtggt gttgattacg
    16381 ggtggtactg gcctgacgga aggagatcag gctcccgaag cgctgctgcc gttgttcgac
    16441 cgtgaagttg aaggttttgg tgaagtgttc cggatgttgt cgttcgaaga gattggcact
    16501 tccacgttgc aatctcgtgc ggtagcgggc gtcgctaaca aaacgctggt tttcgccatg
    16561 ccgggttcga ccaaagcgtg ccgtaccgca tgggaaaata tcattgcgcc gcagctggat
    16621 gcccgtacgc gtccgtgtaa tttccatcca catttgaaga aataagtatg tcgcaactga
    16681 cccatatcaa cgccgctggc gaagcgcaca tggtggatgt ctccgccaaa gcggaaaccg
    16741 tgcgtgaagc gcgcgccgaa gcctttgtca ccatgcgcag cgagacgctg gcgatgatta
    16801 ttgatggtcg ccaccacaaa ggcgacgtat ttgccactgc gcgtatcgcc ggtattcagg
    16861 cggcaaaacg cacctgggat ctgatcccgc tgtgtcaccc gctgatgctc agcaaagttg
    16921 aagtcaattt acaggccgag ccggagcaca atcgggtacg tatagaaacc ttatgccgcc
    16981 tgaccgggaa aaccggtgtc gaaatggaag cgttaaccgc ggcctccgtg gcggcgctga
    17041 ccatttatga catgtgcaaa gcggtgcaaa aagatatggt gattggtccg gtacgtttgc
    17101 tggcgaagag cggcggcaag tcgggtgact ttaaggtgga agcggatgat taacgttctt
    17161 tttttcgccc aggtgcgcga gttggtggga acagatgcaa ccgaagtggc tgcggatttc
    17221 ccaaccgttg aagcgttacg ccagcatctg gctgcgcaga gcgatcgctg ggcgctggcg
    17281 ctggaagatg gcaaattgct ggcagctgtc aaccagacgc tggtgagttt tgaccattcg
    17341 ctgactgacg gcgacgaagt ggctttcttc ccgccggtaa ccggaggtta agatggcaga
    17401 aaccaaaatt gttgtcggcc cgcagccgtt cagcgtagga gaagagtacc cgtggctggc
    17461 tgagcgtgac gaagacggtg cggtagtcac ctttaccggt aaggtgcgca accataacct
    17521 gggcgacagc gtcaaagcat taaccctcga acactatccg ggaatgacag aaaaagcact
    17581 ggcagaaatc gttgatgaag cgcgtaaccg ctggccgctg gggcgcgtca cggtaattca
    17641 ccgcattggt gaattatggc cgggcgatga aattgttttt gtcggtgtta ccagtgcgca
    17701 tcgcagcagt gcgtttgaag ccgggcagtt tattatggat tatctcaaaa cccgcgcacc
    17761 gttctggaag cgcgaagcca cgccggaagg cgaccgctgg gtcgaagctc gggagagcga
    17821 tcagcaggcg gcaaaacgct ggtagtttat tgttagccgg ataaggcacc gctgcgtccg
    17881 gcaatttcac cgctcagtac atccgtttac gctgcgtcga tgcacagcct catcattttg
    17941 cagtatcctt aagatattcc ttatatcttc aggagaaagt catggacaga ttcccacgtt
    18001 ctgattcaat cgtacaaccc cgggctggct tgcaaactta tatggcgcaa gtctatggct
    18061 ggatgaccgt tggcttgttg ctgacagcat ttgttgcatg gtatgcggct aattccgcgg
    18121 ccgtgatgga gctgttgttt actaaccgtg tctttttaat cggtctgatc atcgcgcaat
    18181 tagcattggt tattgtgtta tcagcgatga ttcaaaagtt gagcgcaggt gtaacgacga
    18241 tgctctttat gctttattcg gcgctgacgg gtcttacgct ttccagtatt ttcattgtct
    18301 ataccgctgc ttctatcgcc agtactttcg tcgttactgc cgggatgttc ggcgcaatga
    18361 gcctgtacgg ttacaccacg aagcgtgatc taagcggctt tggcaatatg ctgtttatgg
    18421 cgttaatcgg cattgtgctg gcatcgctgg ttaacttctg gctgaaaagc gaagcattga
    18481 tgtgggcagt cacctacatc ggcgtgattg tctttgtcgg attgacggcg tatgacaccc
    18541 agaaactgaa aaatatgggt gagcagattg atactcgcga cacgtcgaac ctgcgcaaat
    18601 attccattct tggcgcgtta accttgtatc tggacttcat caacctgttc ctgatgttgc
    18661 tgcggatctt cggcaaccgc cgttaattct ttttcatcat caatatcccg gccatgtgtc
    18721 gggatttttt tcttattaaa aacgacactt aacaagtacc aggtataaaa tatgccttat
    18781 ggacgagcgg aagcgttttc tgtatccata aatgcaaaag tattttgtat gcgtggtttt
    18841 aaatttattg atggtgaata ttaatgtttt ttctaaggac aactaaaaga atggaatcat
    18901 actcgcaaaa tagcaataaa ttagattttc agcacgaagc caggatatta aacagcatat
    18961 ggctcattac cgctttgggt ttggtggcaa ccgcagggct ggcctggggc gctaagtatc
    19021 tcgaaattac ggcaacaaaa tatgattcac cgcaaatgta tgttgctata ggattattat
    19081 tactttctat gtatggctta agtaaagata tcaacaagat aaataccgcc atcgcgggcg
    19141 taatatattt gtttttaatc tctttggtgg cgattgtcgt tgcgagttta gcgcctgtat
    19201 ctgccattat cgtcgtgttc agcactgcgg gatcgatgtt tttaatcagt atgctggcag
    19261 gtctattgtt taaatttgat cctggttctc accgttttat cattatgatg acgttgacag
    19321 ggttggccct ggtaatcatc gtgaatgcca cattaatgag tgaacgaccc atttgggtaa
    19381 taagttgctt aatgattgtc ttatggtcag gcattatctc gcatggacga aataagctcc
    19441 ttgaactgac aggaaaaagc catagtgaag aattatggag tccggttcgt tgtgcttttg
    19501 taggtgcatt aacactctat tactatttta tcggcttctt tgggattctt gccgctatag
    19561 ctataacgct tgtctggcaa aggcatacgc ggttttttca ttagccgtaa atgcacatac
    19621 taaagaactt aactatactt cacatcgccg cttcattttt cgcccgtagc ttcttcgcct
    19681 ggctttccag caacaggtaa cagatcagcg ccagcagcag tgggataaag taatacagca
    19741 cacggtaagc gagcagggcg gcgataattg tgcctttgga ggtatgctcc ccagccagta
    19801 gcgcgataaa caccgcttcc agcacaccaa ttcccgccgg aatatgcacg atgacgccag
    19861 caatactact aaccagcaac acgcccagta caaagaaata gttcacgctt tgaccaagta
    19921 acagccagat aatcgctccc attaccatcc agttaacgct ggaaatcagc atctgggcga
    19981 gggcgaattc ccacgaaggc agcaccagct tttgcccttt gatggtcata tggcggtgct
    20041 tcgcgaaagc gcaaaaccac aaataaaccg cgataatcat cagtaagcca ataccgagaa
    20101 tacgcagcgt agtttgatcg acataccagt gatccggcaa ctccaccacg cctgcggtaa
    20161 agataacccc cgctagcaaa atatagccta gccagttggt ggtaatactg agcgaaaaaa
    20221 tccgcgtaat agtgctgccc ggtaacccca gtcgggagta caaacgatag cgcataccaa
    20281 tgccgccgac ccaggtactg agtgtcaggt tgaaggcgta gcagataaac gacaccagca
    20341 tcacctggcg cttcgccagt ttgtgaccgc agtagaaacg ggcgagcagg tcgtagcaac
    20401 cgtaaatcag ataactgacg accaccagcc cgaccgcact aagcagcgca acgcgattgt
    20461 agtcgcggat gaccttccag acctcttccc agtccacttt tttggcgtag accaccagca
    20521 acacaatcac cgcgataaaa aacagccagg tgaggagttt ctttgctaag cgccagcgcg
    20581 ggtgtgattt actcatcagg gttttacccc cgtgttttca gtttctaccc gatcctgcgt
    20641 ttccattgtc ggttgtgcgg ggggaccaac ctgcgccaga cgtggcgtgt gcgccggaag
    20701 ccagcccacc agcgccggga agtggcgtaa aaagtggaac gccagcacgc ttttggtcag
    20761 gttccaccaa gtgcgtttcg gcagcatggt ctcatcgacc tgctgacaat ctgcggcgat
    20821 aatgccgttc agattatcgc gcagcgtctg gttaaaatta cgatcgtgga tgatgacatt
    20881 tgcttcgaga ttcagtgaca aactgagcgg atcgagatta ctggacccta ctgtcgccca
    20941 gtgatcgtcc atcaatgcca ctttgccgtg gagcgggcgg cggcggtact caaacacctg
    21001 aacgccacct ttaaccagat agttatacag caaacgcgcg ccgactctga caatcggcat
    21061 atccggttcg ccctgaatga tcagtttgat ccgcaccccg cgccgtgccg ctttacgcaa
    21121 ggcgtgtaaa aaacgatagc cggggaagaa gtaggcgttg gcgataatca cttcccgccg
    21181 cgcctgagtg agcattttca gatagtgacg ttcgatatca tcgcgatgtt cttcgttatc
    21241 gcgccagacc agcaatacct gcgcttctcc cggctggcgg ttctcttccg ctttatgatg
    21301 acgtcgccac cagcgtcgcg ccgcgctctg tcctggcagg ttttccagct caaactggag
    21361 aatatcttcg acaatcggcc cttcgaggcg aaccgcgtaa tcctgtttgg cctccgggcc
    21421 gtagctggac atatgctcgg cggagtaatt cagcccgcca ataaaggcga tacgcgcgtc
    21481 gatcaccaca attttgcgat gcatccggcg aaacacattg gtgcgcatac caaaaaggcg
    21541 agggcgggga tcgtagtagc ggaacaccac gccagctgcc gtcagttcat tgacaaactc
    21601 atcgctgaga tccggcgaac cgtagccatc cagcaagact tccgctttta ccccgcgttg
    21661 cgctgctgcc aatagcgccg catgcagctg tttgccgacg tcatcctcaa accagataaa
    21721 cgtttcaaga atgatgcgtt cttgtgcctc tccaatcgcc ttaaacaccg cgggataata
    21781 ttgctcgccg ttttccagca actggatctt attgccttcg cgccagctac atttcataaa
    21841 tgaatctccg cacttaaagg ggcatgatca gaaaggtgtc gccatgtccg cagcggcaac
    21901 gcggttggcg cgctggcgct ggcatttttg acgtagatcc tgtccagtcg tagtagagga
    21961 aattgcaccg gaaacgtgcg cgccgggcgt ccgtgggtgc gggtaaaaat ctcatccagc
    22021 ccggcctgca cttttaacgg atgattagct ttttgccgcc agtcgttgaa atcacccgct
    22081 accaataccg gttcgccgtc cggtagctca ttcacccatt cggcgagcat cgcaagctgc
    22141 gcctggcggt gcgcctcacg caggcccaga tgtacgcaca tcacatgaat cgctttcccg
    22201 gtcatcggcg gcacaatgcg gcagtagagc acgccgcgct tttccgcacc atcgaccgaa
    22261 acatcacgat tctcataatg ttcaatggga taacgtgaaa gaacggcgtt accgtgatgt
    22321 ccttccgggt atacggcatt gcgaccgtag gcgaaatcgc tccacatggt gtcggcgaga
    22381 aactcgtagt gcgaggtatc gggccagttt tccacatgca gcggatgaac ttcgtgcgcg
    22441 cccatcactt cctgcaggca aacaatatcg gcgctgacgg tacgcacggc gtcgcgaagt
    22501 tccggcaaaa tgaagcgtcg gttaaacgcg gtaaagcctt tgtgaatatt gatggtgagc
    22561 accttgaacg aaaattgttg tgtttgatcg ggcataattt tcctgtcttt gcctcttatc
    22621 tcattgaaat agtgtagtcg gtgtcacaaa aaggtgcggt cttacggaat tttccgtaaa
    22681 gttcggtact ctgaataagt agagataaat tcttcaggag agaagccatg aagtggcaac
    22741 aacgtgttcg tgtcgcaact ggcctaagtt gctggcagat tatgttgcat ttactggtag
    22801 tggcgctgct ggtggtgggc tggatgagta aaactctggt tcacgtcggc gtaggattat
    22861 gcgcactgta ttgtgtcacg gtagtgatga tgctggtgtt tcagcgccac cccgagcaac
    22921 gctggcgtga ggtggcagac gtgctggaag agctgaccac gacctggtat tttggtgcag
    22981 cgctgattgt gctgtggctg ttgtcccgcg tactggaaaa caactttttg ctggcaattg
    23041 cagggctggc aatccttgcc ggcccggcgg tggtgtcttt gctggcgaaa gataagaagt
    23101 tacatcacct tacgtctaaa catcgcgtac gccgctgacc ctgtcgtggc cgttatcacc
    23161 agtagcggcc acaaactatt ccacacaata tccagactcg catccttcaa ataaatctgc
    23221 ttggtaatgt ccgtaaagtg gcgaataggg ttaatccacg tcagattttg cagccacatt
    23281 ggcatgtttt ccaccggcga aacgtaaccg gaaaggagaa tggcgggcat cataaagaca
    23341 aacacgccga taaacgcctg ctgttgtgtt gaacagagtg atgaaatcaa cagaccgaat
    23401 cccaccagtg ataaaccgta aaccaccatc gtaaagtaga acagcgccag cgatccggcg
    23461 aagggaattt gatacgccca gataccaatc gccagcacaa tggtggcctg gaaggtggcg
    23521 acaattaatg ccggtacggc tttgccgatg aatatctgcc aggtggtgag cggcgaaacc
    23581 agtaactgat cgagcgtacc ttgttcacgt tcgcgggcga cggaaagtga agtgacgatc
    23641 attacgccga tagtggtgat catggcgatc agcgacggca ccacaaacca tttgtagtcg
    23701 agattcgggt tataccagtt gcgtaccacc agctcgctgt tgttgggttt cggttttcct
    23761 tccagcagct cctgctgata atttttgacg atctgttgca ggtagttggc ggcaatttgc
    23821 gcactgttgg agttacgccc gtctaggatc aactgtaacg gcgcggtctg gaaggtatcc
    23881 agtttgcgcg agaagtcagc cgggaaacgc accagtagta acgccttttg tgtgtcgatg
    23941 gtcgggcgga tctcctgcgg gctttttagc agcagcacat gggtaaaggc gctggcgcgg
    24001 gcaaaacgtt gagtaagttc caccgaatgc tcgccgttat cttcatcgta gatggcgatg
    24061 gtggcgttgg tcacttccag cgtggcggcg aacgggaaca ggatcacctg aatcagcacg
    24121 ggtaaaatca gaattgcgcg ggtttgcggt tcgcgcagca gcgactgcaa ctctttgcgg
    24181 attaacgtcc ataagcgatg aaacatgctc ttctccctaa tccagccgac gtttggtttt
    24241 cagccacgtc aggccgataa acatcaccgc cgaagcgatc aaaaacagca cgttcaccac
    24301 cagtaccacc ggaatattcc cggcgaggaa caggctttgc agggtgctga cgaaataacg
    24361 tgcgggaata atgtacgtca ccgcgcggat caccgcgggc atactgtcta tctgaaatat
    24421 aaagccggaa agcataatcg acggcagaaa agccgcgttc agggccacct gagcggcgtt
    24481 gaactggttg cgggtaatcg tggaaatcag cagccccatc cccagggtac tgagtaaaaa
    24541 caggctggag ataaaaaaca gaatcagcag cgacccgcga tacggcacgc cgagaataaa
    24601 caccgacacc agcatacaca gcaacatcgc cagcatcccg agaaagtaat aagggatcag
    24661 cttacacagc agcagctccg tgcgggtaat ctccgtagag agcaaagcct ccatagtgcc
    24721 gcgttcccat tctcgcgcca ccaccagcga ggtgagaatc gcgccgatga ccgtcatgat
    24781 aatggtcacc gcaccgggga taataaagtg ctggcttatc gccgccgggt taaaccagta
    24841 gcgggtttgt acatcaataa gcggttcaaa agtctgcccg ttatcctccg cgcgctgcat
    24901 ctgccagatc tgccagatcc cttcgacata cccctgcaca aagttagcgg tattcggctc
    24961 actgccgtcg gtgatcacct gaatcggtgc ggtggcgttg gcgcgctcca tctgttcggc
    25021 gaaatccacg ggaataacca ccagaccgcg aattttcccc gcctgcattt tggcgatcaa
    25081 ttcctgacgg ttatcgctga tggtggcgtc gatgtagggc gaaccggtca tggtgtgggt
    25141 gaaatccagc gccgcttcgc tacgctgttc cagtaaaatc ccgacccgca gcttgctgga
    25201 gtcgaggtta atgccgtaac caaaaataaa cagcagtagc agcgggatca ccaccgcaat
    25261 cagccagcta ctcggatcgc gaacgatctg ccgcgtctct ttgaagcaca gcgcccgcac
    25321 gcgacgccag gacaggatcg gattactcat tgccatgctc cttatcccag tcgtggatca
    25381 actgaataaa ggcctgctcc attgtgggat cgggttgctc gtcgttggcc gactgcgctt
    25441 tcaaatcatc cggcgtgccg ctggcgatta atttcccgcg gtacaccagg ccgatgcggt
    25501 cgcaatattc cgcttcatcc ataaagtggg tggtgaccat caccgtgacg cctttctcta
    25561 ccatactgtt gatatgtagc caaaattcac ggcgggtgag gggatcaacg ccggacgtcg
    25621 gttcgtcgag aaacagaatg tccggttcat gcatcagcga acaggccagc gccagccgct
    25681 gtttaaaacc taatggcagt tcatcggtcg cgtgggaggc gatactgttc aggccgaacg
    25741 cctcgctcat acgggagatt ttttcgttct gcgcccgacc gcgtaagcca tacacaccag
    25801 agaaaaagcg taaattctgt tcgaccgtca ggttgccgta gagcgaaaat ttttgcgcca
    25861 tatagccgag atgctggcgc gctttaccag aactctcttt cagatccatg cccagcacca
    25921 gcgcctgacc agaggtcggt accagcaaac cgcacatcat cttaaaggtg gtcgatttac
    25981 ccgcgccgtt tggtccgagc aaaccaaaaa tctccccgcg tttaacggca aagttgacgt
    26041 gatcggtggc ggcaaaatca ccaaatttct tggttagttc cttcgcttcg atcaccgtct
    26101 cgccgggtgt gccttctacc gtatgtaata ttgcgcccag cggcgattcc gaggttccgg
    26161 caccgcccag caaatcaata aacgcatctt caaaacgcgg cgtagtttcg ttgatgtcga
    26221 tttccggcat cccgtcggca tggcgaatat cgtctggcgt ggtctctttt ttgaggatca
    26281 gacgtaccga tttcccctga atcatgccgt cgctgacctg cggcagtttc aaggcccgtt
    26341 gcaacagttt gcggttgccc tcgtgcgggc tggtcatcag aaagctgcgt ccggccatag
    26401 tttgagtcag ggccgtcggt tctccctgat acagcagctc gccttcgttc atcagtaaca
    26461 cgtcacggca ctgctcggct tcgtcgagat acgaggtact ccagaggatt aacatccctt
    26521 cgcccgccag ctcatgcacc atctgccaca gttcgcgccg tgagataggg tcaacgccga
    26581 cgccgggttc atcgagcagc aacactttcg gttcgcccac cagggtacag gccaggccaa
    26641 gtttttgttt catcccaccg gagagcttgc ccgccaggcg tccggtaaac gggccaagag
    26701 atgtaaactc cagcaggcga gcaaaagtct gcttccgtgc ctcgccggtg acgctgcgca
    26761 aatccgcgta cagattgagg ttctccatca ccgtcagatc ttcatacagg ccgaatttct
    26821 gcggcatata gccgagcacg gcgtgcagcg cgccgtcgtt tttgatcgga tcaaagccaa
    26881 tcaccgtggc actgccgctg tcgggtttca gtaatcccgc cagcatccgc atcagcgtgg
    26941 ttttgcccgc accgtccggc ccaaccagcc ccgtcacata accggcgtga atggtgcagt
    27001 cgagcggtgc gacggcgggc ttgtccatgc ccggaaagcg tttttccagg ccgttcagcg
    27061 tgataacggc atcattcatg tcctgcctcg tcaccgaatt gcaccgttac cggcattccc
    27121 tggcgtaacg catcatcggc gtcggtcacc acaatacgca ggcgatagac gaggtcggta
    27181 cgcagatccg gtgtttcgac ggttttcggg gtaaattcag cagtcggcga aacgaaacca
    27241 atctgcccgt gatacggttt gttcggacga ccatcggtat aaagcagcac tttgcgcccc
    27301 ggctgggcct ggtcaagatt acgttcatca acataggcgc gtacccacac cggacgcgtc
    27361 agcgagacgg taaataccgt gccaccttca ttaagcaccg tgcctggctc taccgcgcgc
    27421 gttaacagcg tgccatcaga cggtgctatc aacgttgagt cctgcaaatt cagctccgcc
    27481 tgcgccagtt gcgcctgcgc ttgttcgagg ctggctttcg cctgggcgat gtcctgttcg
    27541 cggttaccgg aacggtactg acgcaattta tcctgcgctg atttcagcgt ggcctgcgcc
    27601 tggtcgcgcg aggagcgggc attttccagg tcatttgccg aaatggtgcg gcttttccac
    27661 agtccttgct ggcggttata gaagttctgc gcatagtcat aggcagcttg cgcctgtttc
    27721 accgctgcgg cggactgagc gatttcttca tctcgatacc cggcaagcat caggtcatac
    27781 tgcgcctgag ccaccgaaac acccgctttc gcctgcatca gggcaatctc atacggcttg
    27841 tgatccagtt cgcccagcac ctggcccgct ttgatcgcat caccttcgtc caccgccagc
    27901 gactcaacgc gccccccaac acggaaacta agattcaccg tacgaatatc cacgttgcca
    27961 tacagcgtca ggccgttatc ctggcggctt tgataccacc agtagcctcc ggcaaccacg
    28021 gcggcaagta ctactaccgc cagtccgatc acgacaggtt ttttcatcac tacaaactcc
    28081 tttgcgataa tccttgcaaa atcaggtcaa tatgacaggt caccgtctgg ttgatcagtt
    28141 cggtcttttc ttcatcgaac gcggtccagc cggtacgtaa cagaatagtt tctttgccaa
    28201 gacgaaacgc cagaatctca ccaatcaacg catgggtatg aaggatcatg cgggtatcgc
    28261 tagcgtcgca gccggtccag gcggcaatca ggcgtgtaag gtgactgtgt agcggactaa
    28321 tcacctgttc gtgcaccagg tggtaggctg ccgtgggaga gagctgctca cgggagataa
    28381 atttgctgag gttgacggtg tcatcctggg tgagcagctt aatcatgttt ctgcaggcgc
    28441 gaaggatcag ttcacggatg gctgcccgat ccggctgtgg ttgtgcgaac aaacgttcgg
    28501 cttcttcggc gtgtggacgg aactgctcgc caataaaatc agcaatccac tgggcgctgg
    28561 cgaggtacaa atcttctttc gaaccgaagt agtaagtgat ggctgcgata ttctgcccgg
    28621 cctgggcggc tatctcgcgg gtggtggcgt tcattccata ttcaccaaac tgtgccagtg
    28681 ccgcagcaat cagctgtttt ttcgcctgtt cacccttgat tgtcatggca ggattattca
    28741 tcgcacagtc cattcttaat caaatgattg attaagatta tgactccata ggggagttgt
    28801 ccagtatggc taagaatttt agctacgcca gtcacaggga taatttatgc gctgcgtcac
    28861 aaaaactgct acactccgct ccctcatgac attgtggttt ttgtcatttt ccttttcagt
    28921 atctccctga aaactacacc ggtaacggtc ggggcggttc ggagtagtta tgtctttcga
    28981 ttctttgggt ttaagccctg atatcctgcg cgccgttgcc gagcagggtt accgtgaacc
    29041 cacccctatt cagcagcagg cgatccctgc ggtgctggaa ggccgcgacc tgatggccag
    29101 cgcccagacc ggtaccggca aaacagcggg ctttacgctg ccgctgttgc aacacctgat
    29161 cactcgccag ccgcacgcca aagggcgtcg tccggtacgc gcgctcattc ttaccccgac
    29221 ccgtgaactg gcggcgcaga ttggcgaaaa cgtccgtgat tacagcaaat atctgaacat
    29281 tcgttcgctg gtggtgtttg gcggtgtcag cattaaccca caaatgatga agttgcgcgg
    29341 cggcgttgat gtgctggtgg caacgccggg acgtctgctg gacctggaac atcaaaatgc
    29401 agtgaagctg gatcaggttg aaatcctcgt ccttgatgaa gctgaccgca tgctcgacat
    29461 gggctttatc cacgatatcc gtcgcgtgtt aacaaaactg ccagcgaagc gccagaacct
    29521 gttgttctcc gcgaccttct ctgacgatat taaagccctg gcggaaaaac tgttgcacaa
    29581 cccgctggaa atcgaagtgg cacgccgcaa tactgcgtct gatcaggtga cccagcacgt
    29641 tcactttgtc gataagaaac gcaaacgcga attgctgtcg catatgattg ggaaagggaa
    29701 ctggcagcag gtgctggtgt ttacccgtac caaacatggc gctaaccatc tggctgaaca
    29761 gcttaataaa gacggcatcc gtagtgcggc gatccacggt aataaatcgc aaggtgcgcg
    29821 tactcgtgcg ctggccgatt ttaaatcggg cgatattcgt gtactggtgg caactgacat
    29881 cgctgcgcgc ggtctggata tcgaagagtt gccgcacgtc gtcaactatg agttaccaaa
    29941 cgtaccggaa gattatgttc accgtatcgg gcgtactggc cgtgcggcgg caactggcga
    30001 agcattgtcg ctggtgtgtg ttgatgaaca caaactgctg cgtgatatcg aaaaactgct
    30061 gaaaaaagag atcccgcgca ttgcgattcc ggggtatgag ccggacccgt caatcaaagc
    30121 cgaaccgatc cagaacggtc gccagcaacg tggcggcggt cgtggacagg gcggtggtcg
    30181 cggtcaacag caaccacgcc gtgcggaaag tggtgcgaag tctggtaacg cgaagcccgc
    30241 agaaaaaccg tctcgtcgtc tcggcgatgc caaaccggca ggcgaacaac aacgtcgccg
    30301 ccgtccgcgt aaacctgccg ctgcgcagta atcttttatg ccgggctatg cccggcatga
    30361 gactgatgac aaacacaaaa ctgcctgatg cgctacgctt atcaggccta cgtgaactct
    30421 gcaatatatt gaatgtgcat gtttttgtag gccggataag gcgctcgcgc cgcatccggc
    30481 atttcacaac aagcacttgt cagccatttg cgagcgcggc aaaaagattt ctctgtttta
    30541 acgcccacaa gccacccgat gcgttacttc tccgcagcca attgtccgcg caacgccatt
    30601 aaaaggtacc ccaggcgatt cttgccctga ccattaccgc catctcccca gtaagcatca
    30661 ttttgcgtat gctcaaccag ttttgcggac gcggttgcca gcaagagtgt acgcagttct
    30721 gcatgttgtt cgaatttagc gcgaagcgct ttacgcatca tcctgctggt tattgcgagc
    30781 cgctttccag aaacagaaaa accattaccc ctgaaaaccg aaaaatgcca caatattggc
    30841 tgtttataca gtatttcagg ttttctcatg gcattaaccg ccgcgcttaa agcgcaaatt
    30901 gccgcctggt ataaggcgct tcaggaacag atccccgact ttattccccg tgcgccgcag
    30961 cggcagatga ttgcggacgt cgccaaaacg ctggccggag aagaagggcg gcatctggcg
    31021 attgaagccc ctaccggcgt tgggaaaacg ctctcctatt tgatccccgg catcgccatt
    31081 gcccgcgaag agcaaaaaac gctggtggta agtaccgcca acgtggcatt gcaggatcag
    31141 atctacagca aagatttacc gctgctgaaa aagatcattc ccgatcttaa attcactgcc
    31201 gcttttgggc gtgggcgcta cgtttgcccg cgtaatctga cagcgctcgc cagcactgag
    31261 cccacgcaac aggatctgct ggcgtttctt gacgacgaac tgacgccgaa caatcaggaa
    31321 gagcaaaaac gttgtgcgaa gctgaagggc gatctcgaca cttataaatg ggatggtctg
    31381 cgcgatcata ctgatatcgc tatagatgat gatctctggc gtcgtttgag taccgacaag
    31441 gccagttgcc ttaaccgcaa ctgttattac tatcgcgaat gcccgttttt tgtcgctcgt
    31501 cgggaaattc aggaagcgga agtggtggtg gcaaaccatg cgctggtgat ggcggcgatg
    31561 gaaagcgaag ccgtattgcc tgacccgaaa aatttactgt tggtgctgga cgaaggtcat
    31621 cacctgccgg atgtggcgcg ggatgcgctg gagatgagcg ccgaaatcac tgcgccctgg
    31681 taccggctcc aactggacct gttcaccaaa ctggtcgcca cctgcatgga gcagtttcgc
    31741 ccgaagacca tcccaccgct ggcgatccct gaacgtttga atgcgcattg tgaagagctg
    31801 tatgagctta tcgcctcgtt aaacaacatt ctcaatctct acatgcctgc cgggcaggag
    31861 gcagagcacc gttttgcgat gggcgaactg cctgatgaag tgctggagat ctgccagcgg
    31921 ctggcaaaac tcaccgagat gctgcgtggt ctggcggagt tatttcttaa cgatttaagt
    31981 gagaaaaccg gcagccatga cattgtacgc ctgcatcggt tgattttgca gatgaaccgc
    32041 gcgttgggga tgttcgaggc gcaaagcaaa ctctggcggc tggcgtctct ggcgcaatct
    32101 tccggtgcgc cggtgaccaa atgggcgacg cgggaagagc gcgaagggca gctacacctt
    32161 tggtttcact gcgtgggaat acgtgtcagc gatcagctgg aaaggctgct gtggcgcagt
    32221 attccgcaca ttattgttac ctccgcaacc ttgcgttcgc tgaacagttt ttcgcgtttg
    32281 caggagatga gtgggctgaa agagaaagcg ggcgaccgtt ttgtggcact ggattcccct
    32341 tttaaccact gtgaacaggg caaaattgtt attccccgga tgcgcgttga gccttccatc
    32401 gacaacgaag agcagcacat tgccgaaatg gcggcctttt tccgtgaaca ggtggagagc
    32461 aaaaaacatc tcggtatgtt ggtgctgttt gccagcgggc gtgcgatgca gcgctttctc
    32521 gactatgtga cggatttacg cctgatgttg ttggtgcagg gcgatcagcc acgttatcgc
    32581 ttagttgaac tgcaccgcaa acgcgttgcc aacggtgagc gtagcgtgct ggtgggctta
    32641 cagtcctttg ccgaaggtct tgatctgaaa ggcgatctgt taagccaggt gcatatccac
    32701 aaaatcgctt ttccgcccat cgacagcccg gtggtgatca ccgaaggtga atggctgaaa
    32761 agcctcaacc gctatccgtt tgaagtacaa agcctgccga gcgcctcgtt taacctgatt
    32821 cagcaggttg ggcgactgat tcgaagccac ggttgctggg gcgaagtggt gatttacgac
    32881 aaacgcttgc tgaccaaaaa ttatggcaag cgactactgg atgcattacc ggtatttccg
    32941 atagagcaac cggaagtccc tgaaggtata gttaaaaaga aagaaaaaac gaaatcccca
    33001 cgccgtcggc ggcgttaatg atgtgagtca ggtaaggagt cgtaaatgga ctatcgcaaa
    33061 atcattaaag agattgggcg cgggaaaaac cacgcgcgcg atttagaccg ggatactgcg
    33121 cgcggtctgt atgcccatat gctcaatggt gaagtccctg accttgagtt gggcggcgta
    33181 ttgattgcgc tacgtatcaa aggggaaggg gaagcagaga tgctcggctt ttacgaagcc
    33241 atgcaaaatc acaccatcaa gctgacgccg cctgcaggca agccgatgcc gattgtcatc
    33301 cccagttaca acggcgcgcg taaacaggcc aacctgacgc cgttgttggc gattctcctg
    33361 cacaaactcg gttttcccgt ggtggttcac ggggttagcg aagatccaac ccgcgtgctg
    33421 acagaaacca ttttcgaact gatgggtatt acgccaacgt tgcacggcgg gcaggcgcag
    33481 gcgaagctcg acgaacatca accggtgttt atgcctgtcg gcgcgttttg cccgccgctg
    33541 gaaaaacaac tggcgatgcg ctggcggatg ggcgtgcgta acagtgcaca taccctggcg
    33601 aaactggcga cgccatttgc cgaaggtgag gcactgcgtc tttccagcgt ttcgcatccg
    33661 gaatacattg gacgcgtcgc gaagttcttt agtgatatcg gcgggcgggc gctactaatg
    33721 catggtactg aaggtgaagt gtatgctaat ccgcagcgct gcccgcagat caatctcatt
    33781 gaccgtgaag ggatgcgggt actgtatgaa aaacaggaca ctgctggtag cgagttactg
    33841 ccacaagcaa aagatccgga aaccacggcg cagtggattg agcgttgcct tgctggcagc
    33901 gaaccgattc ccgaatcgct gaaaatccag atggcttgct gcctgatggc tacgggtgaa
    33961 gcggcaacta tcagcgacgg cctggcgcgc gttaatcagg cattttaatt tttatctcag
    34021 ccggatgagc catgtcatcc ggcttttacc ccaccgcaat atgaaattcc tgcatcttta
    34081 ttgaccttcc cgcgcccggc gtgcagcata aaaaaacaac aaacacataa cataaacagg
    34141 agttaaccat ggtaagtggt catcgctttg atgctcagac gctgcacagt tttattcagg
    34201 ctgtatttcg tcagatgggt agtgaggaac aagaagcgaa attagtcgcc gatcatttaa
    34261 ttgcggcaaa cctggcaggg catgattcac atggtattgg catgatccca agctatgtgc
    34321 gctcctggag tcaggggcat ctgcaaatta accatcatgc caaagtcgtt aaagaggcgg
    34381 gggcggcggt cacgctcgat ggcgatcgcg catttggtca ggtcgcggca cacgaagcga
    34441 tggcgctggg gattgagaaa gcgcatcagc acggcattgc cgccgtggcg ctccataact
    34501 cgcatcatat cggccgtatc ggttactggg cggagcagtg tgcagcggcg gggtttgtct
    34561 ctatccactt tgttagcgtg gtcggtattc caatggtcgc gccgttccac ggtcgcgaca
    34621 gccgctttgg caccaatccg ttctgtgtgg ttttccctcg taaagataat ttcccgctgt
    34681 tgctcgatta cgccaccagc gccattgcat ttggcaaaac ccgcgtcgcc tggcataaag
    34741 gcgtccccgt gccgccaggt tgcctgattg acgttaacgg cgtgccgacg acaaatccgg
    34801 cggtaatgca ggagtcgccg ttgggttcgc tgttgacctt tgccgaacat aaaggctacg
    34861 cccttgcagc gatgtgtgaa attcttggcg gggcgctttc tggcggtaaa acgacgcatc
    34921 aggaaacgtt acaaaccagt cccgatgcca ttcttaactg catgaccact atcatcatca
    34981 acccggaact gttcggcgcg ccggattgta gtgcgcagac cgaagccttt gccgagtggg
    35041 tgaaagcctc gccgcatgat gacgataagc cgattttgct cccgggcgag tgggaagtga
    35101 acacgcgtcg cgaacggcag gagttgggga tcccattgga tgcgggaagc tggcaggcca
    35161 tttgtgatgc ggcgcggcag gttggtatgc cggaagagac gttgcaggct ttctgtcagc
    35221 agttagccag ctaaaaaaaa gcccgtccag tggcggacgg gcaaacaagg gtaacatagg
    35281 atcaatgagg gttagagcat atgcgcctgt cggcaaacag acagggaaat acttgtgctg
    35341 aacgtagcgt aaacgcctga tccagccaac ggttcggatt ttgtaggtct gataagacgc
    35401 ggtagcgtcg catcaggcat ctgttgccgg atgcggcgta aacgccttat ccgtcctacg
    35461 aattacttat aaattaccgc agtaccgctt aatttattgt tgttggtggc agaagtgatg
    35521 ctgtatccac tggcaccggc cgccgcggct ttctcagcca gtttcgcttc cagggcatcg
    35581 agggtggatg cgccatcggc agaaaccacg ccgattttat tcatgttctg tgcctgggat
    35641 gccgttaccg gttccgccgc gaacacgcca aatgacagag ttgaaagagc catagcagca
    35701 acaacagtat tgatagtttt cataattaat ctctcgcagg tgatttttgt ataagtgacg
    35761 ttgtttcgtc gatgtgatga gtatcacgtt tttttacgag agataaaatc gaagagaatt
    35821 gaccgccttg ttcaaataaa ttgattgata tctaacatac tgataaacca tttatttgtt
    35881 gtatttatga atccgttttg taccgcgtgg tgctcacggc agcgttaaag gcacggaatg
    35941 ccacgcaaaa tacaaattac actcactaaa catcccgtca gttgcagtta acgtaactgg
    36001 ctgtctttcg aacctctgcg attatatcct gtataagctg gtttttgtaa atctttctcc
    36061 tgttggcaat gaatgcataa gcgcacgcca ggaatggctt cccgacgggc ctgcgggata
    36121 ggggcaccgc actcttcaca ttcatacagg ctttcgccac gcggaatttc accccgagcg
    36181 cgggcaatcg catcttcaat tgtactgttg atctgttcgt tgacggcgtc atcattagcc
    36241 caaccggatg ccatatcgtc ctccccatat caatacttgt acagttaagt gtagctaata
    36301 tagggacgaa ctcgggcagt tcaagcgtca gatctccgac cattcccgca gcagattatg
    36361 ataaagattg agcagtgaca ggatctcttc actttcgccg tagcggcttt tcagcgactg
    36421 aatattgttg tccagttcaa acagcatggc gcgcttttta tcatcacgga tcatcgactg
    36481 gatccacata aatgatgcca ctcgcacgcc gcgggttacg ggtgtcacgc aatgcaggct
    36541 gctggaggga tacaacacca gatcgcctgc cgggagtttt acccgatgtt gcccgaaggt
    36601 gtcattaacg accagttcgc cgccgtcgta gctttctgga tcgcttaaaa acagcgtggc
    36661 agaaaggtca gtgcgcatcc agccgttttg tggatggctg cgtactgcgc catccacatg
    36721 aaaaccatag gtttcattgt tctgatagcg attaaacagc ggcgtggaaa gggtacgcgg
    36781 caaggccgcg gcaaagaaca aagcatgttg gttaaccgcg ttcagcacct cattttgcag
    36841 ggcggcgtac agggcgctgc gggtgtctac ttgttgattg ttcttaactt gcgcaccttg
    36901 tgcgccggtg gtgacgcgcc catccaccca ttcggcttgt tccagttgtt cgcgaaaacg
    36961 ggcgacgtcc tgtggcgata gcacgccggg aatgtggtac atcatacaag ttctccaaaa
    37021 agtggggcct gcgccccaca tctcaatcag aaatgcatat tggccgtgag caagaaggtt
    37081 cttggctcgc ccgggtgata acggtagccg ctcttattga ttgaggcgac gtaatcggta
    37141 tcaaacaggt tgtagacgtt tagctggaag tcgagattgc gattaacccg ataccccagt
    37201 ttggcgtcgg cgacccaata accttcggta aacgctggcg ttcccaccgc gccgtctgaa
    37261 cctttatgca tactgccgat ataccgtgcg cccgcaccaa cagaaatatc atcggttgcc
    37321 tgatactggc tccataaggt gaaggcgtgt tccggggtat acggcagcga tgaggaacca
    37381 tcctgggcaa catctttgcc gtttttgata gttgcttttt gctgggtata gccgccaatc
    37441 atctgccatg cgggggtgat attcccggcc acggatatct cgtagccttc gacacgtttc
    37501 ttaccatatt gcgaataagt cccgtcatca ttttgctcaa cttcattttc aatatcagta
    37561 cggaacagcg cggcggtaag tagcaggcgt ttatccagaa cctgccattt ggtgccaatc
    37621 tcgctggtgt tggctttttg tggtttaaaa tcggtgcggt tggcactgtt accgctgcca
    37681 gactgcgcaa gggcgaagtt gttgccgccc ggcggctgct gggaaacggc atagttaata
    37741 tagacattgc cgttttccgt caggtgatac agcgccccgg ctttccagtt caccagattg
    37801 cccgacttgg cggtgtcgac ggtggtgacc ggagaacctt ttgccacgcc agcagggcag
    37861 gtgatggcac cgcgcccgct gccgccgcag gcggtagcac tgtcatattc agtatgataa
    37921 ttatccagac ggatcccgcc gttcagctca aaatcacggg tgatttgcag cgtatcgaag
    37981 gcataaattg cgaaggtatc cgtctgacca ttggcgtttg cgccgttgcg cgtcaggccg
    38041 ccgggatgaa tgctgctgtc aggatgataa atatttaccg ccggtaacgt caccggatta
    38101 acgccatagt tagtctgtgt ttcgcgggta aattccacgc cggtactgac atcatgaccg
    38161 atagcgccgg tatagaacgt cgaggtcagg ttggtctggt tggtgagaat tttgttactc
    38221 acatctttgg tattcgccgt acgtgaccac gtccagctat tcacatcgct ggtgggctgg
    38281 gtaatattcg acgctccacc cataatcgcc gttatcaggt aatcctgctt tacgcgcgac
    38341 caacgcgtag tgttgcgaat ggtggtgtta tcgttgatgt cgtgctcaaa acgcatggta
    38401 gcggtgtcgg tggtcgaatc gtcgtaatcg gaatccgtgc cgtaaaagtt atgagtatca
    38461 acttttccgg aatgattcag ggccgctgtt cccgcagatg gggcagaata acccggtaaa
    38521 ccgatggtcg gaatgccgcc gtctggcgtg ttgtgttggg tgacatgcag ataattaaga
    38581 tacaaacgat tcgcggtacc aaggccaaaa gcgacagaag gggcgacgcc gtaacgctca
    38641 tttttgactt tgtcgcgtcc ggcatcgtgc gttttttcgc ccattacatt caggcgcacc
    38701 gcagtggtat caccaatgac ctgattgacg tccagcgtgc cgcggcggaa ccaggcgctg
    38761 ccaatactgg cggaggcgtc aatgccggaa tcattgcgcg gctgtttgct gatcatattg
    38821 atcgagcccg tcggcgcgct gcgcccgtag tcggtgccgg acggcccttt aattacttcg
    38881 acctgctcgg tattgaaggt gtcgcgcgag acgctgccga tatcgcgaat gccatcgata
    38941 taaatactgt tagaggtatc ggcaccgcgc atataaatgg cgtcgccagt ggtggagtta
    39001 ccgttctcac ccgcaaaaaa cgcacccacg ccggggacgt ttttgagcgc gtcggtaagg
    39061 ttggttgcac cctgatcttt aatcacttgt tcagaaatca ccgtcatcgt gcgggtagta
    39121 tccgctaccg gacgcgagaa tttcggatcg gcagattgtt gtggcgcata aagcgaaggc
    39181 gttgatgctt cgacaaccag cgtgtcatcc gcgttagctt gcccttcggc agcgagtgcc
    39241 tgcgccacag gtgtaatacc aatacaaaga ccggcaaaga acgtgagcga atgaaattgt
    39301 ctggcaggga aattgcgatt gttttccatt ttgcaggtga ctttttctta tatggtgaaa
    39361 ttttcactgt cacgagaaag catctctgcg gatgcgatgc cactgttaat cccgtgatag
    39421 cgtccgtgca agtgataata attttgataa gcattatcac ttggcgcgat tttctatcac
    39481 ccattgcgcg aaaaagaaat acatttattt acattgcgtt catatttgat gttttttgct
    39541 tacaaatcgg acgaaaactg catccagagg cgaatgcagt tacgttggtg gggttatttg
    39601 tagatgattg cagtaccgaa catctggtca tttccgcctg cggaattaat gacataacct
    39661 ttcgcgcctt gttcgcgcgc
//
LOCUS       DAZGJT010000035        39607 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZGJT010000035 DAZGJT010000000
VERSION     DAZGJT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.035"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..230)
                     /gene="yfgG"
                     /locus_tag="ACHKFK_001996"
     CDS             complement(39..230)
                     /gene="yfgG"
                     /locus_tag="ACHKFK_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO9052342.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            248..400
                     /locus_tag="ACHKFK_001997"
     CDS             248..400
                     /locus_tag="ACHKFK_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052343.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMKVNLIIYND
                     DVIMMS"
     gene            582..2825
                     /gene="pdeF"
                     /locus_tag="ACHKFK_001998"
     CDS             582..2825
                     /gene="pdeF"
                     /locus_tag="ACHKFK_001998"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO9052344.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDSRLISQRLFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAI
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQRALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2864..4405)
                     /gene="ppx"
                     /locus_tag="ACHKFK_001999"
     CDS             complement(2864..4405)
                     /gene="ppx"
                     /locus_tag="ACHKFK_001999"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO9052345.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFHGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4410..6476)
                     /gene="ppk1"
                     /locus_tag="ACHKFK_002000"
     CDS             complement(4410..6476)
                     /gene="ppk1"
                     /locus_tag="ACHKFK_002000"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO9052346.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYELVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGCDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQSE"
     gene            complement(6648..7286)
                     /gene="purN"
                     /locus_tag="ACHKFK_002001"
     CDS             complement(6648..7286)
                     /gene="purN"
                     /locus_tag="ACHKFK_002001"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO9052347.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLITSAFDSREAYDRELIHEIDMFAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITVRVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(7286..8323)
                     /gene="purM"
                     /locus_tag="ACHKFK_002002"
     CDS             complement(7286..8323)
                     /gene="purM"
                     /locus_tag="ACHKFK_002002"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO9052348.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLKLIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8647..9273
                     /gene="upp"
                     /locus_tag="ACHKFK_002003"
     CDS             8647..9273
                     /gene="upp"
                     /locus_tag="ACHKFK_002003"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO9052349.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            9359..10648
                     /gene="uraA"
                     /locus_tag="ACHKFK_002004"
     CDS             9359..10648
                     /gene="uraA"
                     /locus_tag="ACHKFK_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO9052350.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISMLRPEEVVLDAEDADITDK"
     gene            10743..11444
                     /gene="hda"
                     /locus_tag="ACHKFK_002005"
     CDS             10743..11444
                     /gene="hda"
                     /locus_tag="ACHKFK_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO9052351.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11582..11941)
                     /gene="arsC"
                     /locus_tag="ACHKFK_002006"
     CDS             complement(11582..11941)
                     /gene="arsC"
                     /locus_tag="ACHKFK_002006"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO9052352.1"
                     /translation="MTKQAKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEVLIQAMVENPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11962..13425)
                     /gene="bepA"
                     /locus_tag="ACHKFK_002007"
     CDS             complement(11962..13425)
                     /gene="bepA"
                     /locus_tag="ACHKFK_002007"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO9052353.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANDAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNHDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13638..14699
                     /gene="yfgO"
                     /locus_tag="ACHKFK_002008"
     CDS             13638..14699
                     /gene="yfgO"
                     /locus_tag="ACHKFK_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9052354.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRPWATSIVLILFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQD"
     gene            complement(14798..15268)
                     /gene="bcp"
                     /locus_tag="ACHKFK_002009"
     CDS             complement(14798..15268)
                     /gene="bcp"
                     /locus_tag="ACHKFK_002009"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO9052355.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(15268..15840)
                     /gene="gcvR"
                     /locus_tag="ACHKFK_002010"
     CDS             complement(15268..15840)
                     /gene="gcvR"
                     /locus_tag="ACHKFK_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO9052356.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDTHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            15986..16864
                     /gene="dapA"
                     /locus_tag="ACHKFK_002011"
     CDS             15986..16864
                     /gene="dapA"
                     /locus_tag="ACHKFK_002011"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO9052357.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            16881..17915
                     /gene="bamC"
                     /locus_tag="ACHKFK_002012"
     CDS             16881..17915
                     /gene="bamC"
                     /locus_tag="ACHKFK_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO9052358.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSEWHELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            18128..18841
                     /gene="purC"
                     /locus_tag="ACHKFK_002013"
     CDS             18128..18841
                     /gene="purC"
                     /locus_tag="ACHKFK_002013"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO9052359.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKQFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            19009..19872
                     /gene="ypfJ"
                     /locus_tag="ACHKFK_002014"
     CDS             19009..19872
                     /gene="ypfJ"
                     /locus_tag="ACHKFK_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO9052360.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            19887..21902
                     /gene="tmcA"
                     /locus_tag="ACHKFK_002015"
     CDS             19887..21902
                     /gene="tmcA"
                     /locus_tag="ACHKFK_002015"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO9052361.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEERWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARQGFDAAAFAALSGTLKAGSWL
                     VLLLPVWDEWENQPDADSLRWSDCPDPIATPHFVQHFKRVLTANNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALCLVDEGGLSQELSQAVWAGYRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYIHDLDYLSVSFGYTEELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAVLSDDDWLELAGFAFTHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKLLLVRQREEAAQALFALDDVCTERLRDRITQWQFFH"
     gene            21976..22674
                     /gene="ypfH"
                     /locus_tag="ACHKFK_002016"
     CDS             21976..22674
                     /gene="ypfH"
                     /locus_tag="ACHKFK_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9052362.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQLALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(22755..22955)
                     /gene="ypfN"
                     /locus_tag="ACHKFK_002017"
     CDS             complement(22755..22955)
                     /gene="ypfN"
                     /locus_tag="ACHKFK_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO9052363.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(22983..24110)
                     /gene="dapE"
                     /locus_tag="ACHKFK_002018"
     CDS             complement(22983..24110)
                     /gene="dapE"
                     /locus_tag="ACHKFK_002018"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO9052364.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(24114..24470)
                     /gene="yffB"
                     /locus_tag="ACHKFK_002019"
     CDS             complement(24114..24470)
                     /gene="yffB"
                     /locus_tag="ACHKFK_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO9052365.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAATLMTEMPAIIKRPLLCAPGKPM
                     LLGFSESSYQQFFHEV"
     gene            24851..24910
                     /gene="ypfM"
                     /locus_tag="ACHKFK_002020"
     CDS             24851..24910
                     /gene="ypfM"
                     /locus_tag="ACHKFK_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO9052366.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(25009..28111)
                     /gene="acrD"
                     /locus_tag="ACHKFK_002021"
                     /pseudo
     CDS             complement(25009..28111)
                     /gene="acrD"
                     /locus_tag="ACHKFK_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(28275..29975)
                     /gene="narQ"
                     /locus_tag="ACHKFK_002022"
     CDS             complement(28275..29975)
                     /gene="narQ"
                     /locus_tag="ACHKFK_002022"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO9052367.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYRSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGDNWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            30181..32160
                     /gene="aegA"
                     /locus_tag="ACHKFK_002023"
     CDS             30181..32160
                     /gene="aegA"
                     /locus_tag="ACHKFK_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO9052368.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAVGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDAALSGMAKSRRLR
                     TARQEQQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGMAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLNSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            32228..32803
                     /gene="nudK"
                     /locus_tag="ACHKFK_002024"
     CDS             32228..32803
                     /gene="nudK"
                     /locus_tag="ACHKFK_002024"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO9052369.1"
                     /translation="MTQQITLVKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDSQRANAGGGVEDEDIEVLELPF
                     SQALDMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            32929..33972
                     /gene="ypfG"
                     /locus_tag="ACHKFK_002025"
     CDS             32929..33972
                     /gene="ypfG"
                     /locus_tag="ACHKFK_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO9052370.1"
                     /translation="MRYRIFLLFFFALLPMSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDVSEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGDQTISLNGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVVVVNPTPTPLSREERNDLLDYGNWRMNGLRCSL
                     DPLRREVSVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(34049..36052)
                     /gene="tkt"
                     /locus_tag="ACHKFK_002026"
     CDS             complement(34049..36052)
                     /gene="tkt"
                     /locus_tag="ACHKFK_002026"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9052371.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVVHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEKEVALARQKL
                     GWHHPPFEIPKEIYHAWNAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVSARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKILGVKGA"
     gene            complement(36072..37022)
                     /gene="tal"
                     /locus_tag="ACHKFK_002027"
     CDS             complement(36072..37022)
                     /gene="tal"
                     /locus_tag="ACHKFK_002027"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO9052372.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPHDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPIDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            37311..39590
                     /gene="maeB"
                     /locus_tag="ACHKFK_002028"
     CDS             37311..39590
                     /gene="maeB"
                     /locus_tag="ACHKFK_002028"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO9052373.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT         9406 a        10000 c        10399 g         9802 t
ORIGIN      
        1 ggtgccgtta tgttctgtga gagtggagaa ggtaaccgct aacgttgtac cggtgattcg
       61 acggagagtg tggattgctg agcttctttt ttgctctgat ggtgctgcca ggcaccgacg
      121 gacgagtaga taaaacggcc aaagaagaag ataaagctga tgagcagtac gatacgggtc
      181 atgcgactgt taaatcggtg tcgttttcgc atactggttg cctgactcac aaaaggttcc
      241 ttgaagtatg tcccacgccg tggacggtac ttacattaag gcacaacagg acaaaatggt
      301 caattcttcg ttatgtaaaa aagcgtcagt tgatacatat tttaatgtta tgaaagttaa
      361 tttaattatt tacaatgatg atgtaataat gatgagttaa catgataaac gtaataattc
      421 attaatcagg gttattttgt ttgatatata tcaattggaa ctttcatatg acacttagaa
      481 tcattgctct ctatagtgat gaataatcat cattcgaagt caggtgggat gcctgtctga
      541 atacacctcc ttcaggatgt gggggattct gctgagcatc tatgaaactg aatgcaactt
      601 atataaaaat acgtgataaa tggtgggggc ttccgctgtt cctgccttct ttaatcttgc
      661 ccattttcgc ccacattaat actttcgcgc atatttcttc cggtgaagtt tttctctttt
      721 atctgccact ggcactgatg atcagcatga tgatgttttt cagctgggcg gcattgccag
      781 ggatcgcctt agggattttt gtccgcaaat atgcagagct gggtttttac gaaacgctct
      841 cattaacggc taattttatt atcattatca ttctctgttg gggcggttac agggttttta
      901 ccccccggcg taacaacgtt tcacatggtg atagccgttt aatttcccag cgactattct
      961 ggcagattgt gtttcctgca acgctgtttc tgatactttt ccagtttgct gcgtttgtag
     1021 ggttactggc gagcagagaa aatctggtcg gcgtcatgcc ctttaacctc gggaccttaa
     1081 tcaattatca ggccttgctg gtgggtaatc tgattggtgt cccgctgtgc tacttcatca
     1141 ttcgggtggt gcgaaatccg ttttatttac gtagctatta ttcgcaatta aaacagcagg
     1201 ttgatgccaa agttaccaaa aaagagttcg caatctggct actggcatta ggtgctttac
     1261 tattgctgtt atgcatgccg ttaaatgaaa aaagcacgat ttttagcacc aattacacct
     1321 tgtcattatt gctgcccctg atgatgtggg gagcgatgcg ctatggttat aagctgattt
     1381 cattgctctg ggcggtcgtg ttgatgatca gcatccacag ctatcaaaat tacattccca
     1441 tttatcctgg ctataccacg cagctcacca taacctcctc cagttatctg gtattctctt
     1501 ttattgtcaa ttatatggct gtactggcaa cccgtcagcg agcggtagtc agacgcattc
     1561 agcggcttgc gtatgtggac ccggtggttc atctgccaaa tgttcgcgcc ctgaatcgcg
     1621 cgttacgtga tgccccctgg tctgcgcttt gttatttacg catccctggc atggaaatgc
     1681 tggttaagaa ttatggcatc atgctgcgga ttcaatacaa gcaaaaactt tctcactggc
     1741 tgtcaccatt gttggaaccg ggtgaagatg tttatcagct ttcgggtaac gatctcgcgc
     1801 tgcggctgaa tacagaatcg caccaggagc gcattaccgc actggatagc catctcaagc
     1861 aatttcgttt cttttgggat ggaatgccga tgcaaccgca gattggcgtc agttactgct
     1921 atgtgcgctc gccagtgaat catatctacc tgctgctggg agagctaaat acagtggccg
     1981 aactttccat cgtgaccaac gccccggaaa atatgcagcg tcgcggagca atgtatttgc
     2041 aacgcgaatt gaaagataaa gtcgcgatga tgaatcggct acagcgggcg ctggaacaca
     2101 accatttttt cctgatggcc cagccgatta ccggtatgcg tggtgatgtc taccatgaaa
     2161 ttcttctgcg catgaaaggt gagaatgatg aactgatcag ccccgatagc ttcttaccgg
     2221 tcgcgcacga atttggttta tcgtcgagta tcgacatgtg ggtcattgag catacgctgc
     2281 aatttatggc tgaaaacaga gcgaagatgc ccgctcaccg ttttgctatt aatctgtctc
     2341 caacctcggt atgtcaggct cgttttcctg ttgaagtcag tcagctgctg gctaaatatc
     2401 agattgaagc gtggcaactt atttttgaag tcaccgaaag taatgctctg accaatgtta
     2461 agcaggcgca aatcaccttg cagcatcttc aggaattagg ctgccagatt gcgattgatg
     2521 atttcggcac cggctacgcc agctatgcgc ggcttaaaaa tgtgaatgcc gatctgctta
     2581 aaattgacgg cagttttatc cgcaatattg tgtcaaatag tctggattat cagatagtgg
     2641 cgtcgatttg ccacctggcg cgaatgaaga aaatgcgggt agtggcagag tacgttgaaa
     2701 acgaagagat ccgcgaggcg gtgctctctt tggggatcga ttatatgcag ggttatctta
     2761 ttggtaagcc gcaaccgtta attgatacgc tgaatgaaat cgaacccatt cgcgaaagtg
     2821 cctgaataat gcgggccgac atttctcgtc ggcccgcaaa gcattaagcg gcgatttctg
     2881 gtgtactttc ttcttcaatt ttcaaccgcc agccagccac gccttcccag tattcttgct
     2941 ccttttccag atcaagcagt accagcgcat tctgactaaa ccagtcatgc gggaaacgca
     3001 gtgtccagtg actgtcatca gtaatcagcg tcaatgttgg cggtgtggtg gttgcctgac
     3061 gttgattatt gagtaatacg ccaaggcgca atagctgaat cagtggcagg aactgtttct
     3121 tcttaaacaa ggtaaagcgc ggcagatcgt cgagtttaat cgctttacgg tgatagcgca
     3181 ccagtgtcgc catcatcagc tgctgttcct gattaaaacc cggcaagtcg ctgttttgca
     3241 gaatataagc ggagtggcga tgcaaaccgc tgtggttgat attcaacccg acctcatgca
     3301 gcatggcggc ccatcgcagt agcgcctcca gttgtggatg cgccagcttc ggttgctgtt
     3361 cccgccactg ttcgtacatt tgcatagtgg tatccagcac ccgtcgggcc tgttcgctgt
     3421 cgatgtgata ctggttggcg aggctgctgg cagtgcgact acgcacatcc tgatgacgga
     3481 aacgtccttc catttcatac agtacgcctt cgcgtaacgc cccgtcagaa aggcgcagtt
     3541 cacggatggc taaagcatca aacacaccgc ataaaatcgc cagtccagga acgaagactg
     3601 ttttccgctc ttcggaaaga ccaggtaaac tcagcgatgc gaaattacgg tgccgtaaaa
     3661 cttcttttac cagtttttcc agacgttccg gggtaattat cccgtctttc tcgcccattt
     3721 ccattaacac ttcatgggcg gcttttatgg taccggaagc gcccattgcc acgttccagc
     3781 cctgaattcg aaattgccag gttaacgttt ccagtttttg tgctgccgcc atgcgagcgc
     3841 gctgaaaatt ctctttattg atgaccccgc catgaaaata aagctgggca aagctgacac
     3901 aacccatccg gcggctttca acgagaatcg gttcgaaatt ttcaccaatt accagttctg
     3961 tagaaccgcc gccaatatca ataaccagtt tgcgaccttt ttccggttgg gtatgttcca
     4021 cgcccataaa aatcagacgg gcttcttcat taccggaaat aatttcgatc gggtagggaa
     4081 tgactttttc cgcgcgtttc agaaagtcag tggcgttcag cgcctgacgc agcgtatggg
     4141 taccaactat acagacgctg gcaggagaaa acccctgtag cctttcggca aatagcgaca
     4201 gacagttcaa accgcgcgtc attgcctctt cactcaacat attatctggc cccaggccgt
     4261 ccgccagatg cacccgctgt ttcaggcggc caataatctg catggcacca tctaccacac
     4321 gggctatgac catgtgaaaa ctgtttgaac caagatcgac cgcagcaaac tcctgcggac
     4381 gaggggattt atcgtgtatt ggcatagggt tattcagatt gttcgagtga tttgatgtag
     4441 tcgtaaatcg ccaactgcgc ccgtactttg cggcgattgc cgcggggaac gtagcgatta
     4501 ctgagttctt tatcgatata acgtgctttg accgtatcgc tgaacaatat gtcgatgatg
     4561 tccagcaccc gctgcttcag gcgcggatcg agcagcggcg ttgccacttc aatacgataa
     4621 tcaatattgc gcgtcatcca gtcggcggaa gaaaggtaga cctttttatc gcagccattt
     4681 tcaaaaatat aaacccggtc atgttcaagg taacggtcaa caatactgat ggcacgaatg
     4741 ttgtcgctaa tgccttccag attggggatc agagaacaca ttccgcgcac cagcagatta
     4801 accggtacgc cggagctgga ggccgcatac agacgatcaa ccaggccttt atcgacaagg
     4861 ttatttagct tcagggtgat accactgggc agcccttgct gcgcgttggc gatctcgcgg
     4921 tccaccaatt catacaacag acggcgagag ttttgcggcg acaccattaa ataatcaaat
     4981 gtcaccggac ggtatgggtt ttcaataaag ttgaacaccc gccgtacttc gttagtgatg
     5041 cgcgcatcgg cggtcagcaa cgaatagtca gtataaagac gcgcggtttt ttcgttaaag
     5101 ttcccggtcc cgatgtgtgc gtaacgcacc acttcaccgt tttctttacg tgaaatcagg
     5161 aacagtttgg cgtgaatttt cagccccggc gcagagaaga taacgtgcac gcctgcttcg
     5221 gtcaggcgct tcgcccagtg aatgttggct tcttcgtcga aacgcgcctg taactcaacc
     5281 accacggtga ctttcttacc gttgtgtgcg gcgtggatca tcgagtcgat gatgcgtgaa
     5341 tctttcgcca cgcggtaaat gttaatttta atcgccagta cgctcgggtc gaacgaagcc
     5401 tgacgcagca gttccagtac atgctcaaag gtgtgataag gataatagag caacacatcg
     5461 cgttcgcgaa tggcatcaaa accattgcgg aactgggctt tatcaaacca aatatggcgt
     5521 aaacgcggca gtggtttgtt caccagattg gctttgccga cattggggaa attaataaag
     5581 tctttaaaat tatgataacg accgccgggg acgatggagt cgtagcggga aatagtcagt
     5641 ttttcgcgta acacttcaac cagagcattg ggcatatcgc gctgataaac aaaacgcacc
     5701 ggctcagcag ttaaacgctg cttgagactg gaagacatca actccatcag actggcttcc
     5761 atctcatgca ctaaatcgta ttcggcatcg cgggtcatct tcattgaata ggcattcagt
     5821 gcgtcataat caaagaagcc tttgaaaata tcatcaaggc agtaacgcag aatgttatcc
     5881 agaagaatca tcggcttgcg tcgacgcggc gcttctggcg gtaaattcac aaagcgcggc
     5941 actttatctg atgggatctc cagcagcgcg taacggatgg tatcaccacg gataatttcc
     6001 accgccagat aggtgtaatc atctttcagg aactgcacta agtcagtgtc aggattgatt
     6061 aaaatcggcg taatgtgctg acgcagatac tgcttaaaat aatgacgcag ccagttttgt
     6121 tgattgacgg agagctggcg ttcattaatc aggaagatct ggttgcgcgc catctccagc
     6181 agtagctcgt tgtagaggcc gtcgaattcc tgatcggctt tcagcacccg ggactgaatt
     6241 ttgcccagta aatggcggga atgagagttg gaaccttgtt cttcgctaat aatgatgcgt
     6301 cgcttcagtt cagcgaagcg gactttatag aactcatcaa ggttattgga atagatcccc
     6361 aggaaacgca tcctttcaat cagcgggtta gatttgtccg ccgcttcctg aagcacgcgt
     6421 tcattgaacg ataaccaact gagctctttt tcgatgtata gcttttcctg acccattacc
     6481 acttttactc cgttttattc acgggacact gcctggatat tatggcgagc aatattggca
     6541 gatgtccaac tatgccagaa aagggggaaa aaaactgctg gagcttgcga tttacaggtg
     6601 aattgaaaaa cgccagcggc agagctggcg ctttaactac gggggcatta ctcgtcggca
     6661 gcgtagccct gcggcggcag acgttgacca tccagccacg cggcgttttc gtgcattttc
     6721 agacgaccat cggcaaacca gctaatcacc agtggataaa tagcatgttc ctgagtttgc
     6781 acgcggacgg tgatgtcatc ttccgtatca ccagcaaata ccgggacttt cgcctgtaaa
     6841 ataaccgggc caccgtccag ttcatcggtg acgaaatgca ccgatgtacc gtgctcctca
     6901 tcgccatttt ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg cagcagagaa
     6961 gggtgaatgt tcagcaaacg cccggcatag tgggagacaa acgccgggct gagaatgcgc
     7021 ataaaaccag ccagcacgac cacatcgggt gcgaacatgt cgatttcatg aatcaactcc
     7081 cggtcatagg cttcacgact gtcaaacgcg ctggtgatga gcgtatgcgt tgcaataccc
     7141 gcctggcggg cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac tgcccgtacg
     7201 gtgcctttaa ttttgttggt tttgcaggcg tcaataattg cctgcaaatt acttccgttg
     7261 ccggaaataa gcaccacaat attcattatt cgataaccac gcgttgttcg gaatcagagg
     7321 ctttgatgat accgattttc cacgcgtttt caccgttggc attgagcagg gcgagggctt
     7381 tgtccacttc cggagcaggc agggcaataa tcataccgac gccgcagttg aaggtgcgat
     7441 acatttcatg gcgctcaacg ttacctgccg tttgcagcca gttaaacact tccggccact
     7501 gccaggaaga ttcatcaatc actgcctggg tattatctgg caatacgcgc ggaatgtttt
     7561 cccagaaacc gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt tcaatcaatt
     7621 tcagcactga cttcacgtaa atgcgggtcg gtgccagcag atgatcggct aatggcttac
     7681 catcaagttc ggtggtttgc ggatcacaac cgctgacttc aagaattttg cgcaccagcg
     7741 aatagccatt cgagtgcggg ccgctagagc caagtgcaat cagcacatcg ccgtcgctga
     7801 ctttagatcc gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa ccagcgacat
     7861 cgtaatcttc accgtgatac atccccggca tttccgccgt ttcgccaccc accagtgaac
     7921 agcccgattg cagacaacct tccgcaatgc cgctgatcac cgctgaagcg gtatcaacat
     7981 ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg ttccgcacct tgcaccacta
     8041 ggtcattaac gcacatggcg accagatcaa taccaatggt gtcgtgacgt tttaagtcca
     8101 tcgccagacg cagcttggta cctacgccgt cagtgccgga aaccagcaca ggttcacgat
     8161 atttttgcgg caatgcacac agtgcaccga agccgcccag accgcccatc acttccggac
     8221 gacgcgtttt cttcactacg cctttgattc ttccaaccag agcattaccc gcgtcaatat
     8281 caacaccggc atctttgtag ctaagagagg ttttatcggt cactgcttgg gtccccacgc
     8341 gttacttgcg gtagaaaaat aaaattcggc gcaattctaa cagggaaagc aaacgtttgc
     8401 gagactgctt tacacaacct ttttgcacgt cttttcccca ggcgcgcggc gaaagaatac
     8461 ttgtaccagg gcaaaggatc gttttcggat ggaataatct tctttcataa ccatttgaat
     8521 ataaaataac tttatctcaa accgttatca ttttgactaa agtcaacgaa aataatattg
     8581 ccgccttgaa gaaaggaggt ataatccgtc gatttttttg tggctgcccc tcaaaggaga
     8641 aagagtatga agatcgtgga agtcaaacac ccactcgtca aacacaagct gggactgatg
     8701 cgtgagcaag atatcagcac caagcgcttt cgcgaactcg cttccgaagt gggtagcctg
     8761 ctgacttacg aagcgaccgc cgacctcgaa acggaaaaag tcactatcga aggctggaac
     8821 ggcccggtag aaatcgacca gatcaaaggt aagaaaatta ccgttgtgcc aattctgcgt
     8881 gcgggtcttg ggatgatgga cggtgtgctg gaaaacgttc cgagtgcgcg catcagtgtc
     8941 gtcggtatgt accgtaatga agaaacgctg gagccggtac cgtacttcca gaaactggtt
     9001 tctaacatcg atgagcgtat ggcgctgatc gttgacccaa tgctggcaac cggtggttcc
     9061 gttatcgcga ccatcgacct gctgaaaaaa gcgggctgca gcagcatcaa agttctggtg
     9121 ctggtagctg caccagaagg tatcgctgcg ctggaaaaag cgcacccgga cgtcgaactg
     9181 tatactgcat cgattgacca gggactgaac gagcacggat acattattcc gggcctcggc
     9241 gatgccggtg acaaaatctt tggtacgaaa taaagaataa aaataattaa agccgacttt
     9301 aagagtcggc ttttttttga gtaaagcgcc tataacacat aatacagagg ataatactat
     9361 gacgcgccgt gctatcgggg tgagtgaaag accgccactt ttacagacaa tcccgcttag
     9421 tttgcaacat ttgttcgcca tgtttggtgc aaccgtcctg gtgcccgtct tatttcatat
     9481 taacccggcg actgtactgt tatttaacgg tataggaaca ctgctgtatc tcttcatctg
     9541 taaagggaaa attccggctt atcttggttc cagctttgcc tttatttcac cggtattgtt
     9601 actgttgccg ttggggtatg aagtcgcgct gggcggcttt attatgtgcg gcgtgctgtt
     9661 ctgcctggtt tcttttatcg tgaagaaagc ggggaccggc tggctggacg tgctgtttcc
     9721 acctgcggca atgggcgcaa tcgttgccgt catcggtctg gagctggcgg gcgtagctgc
     9781 cggtatggcg ggtttactcc cggctgaagg gcaaacgcca gactccaaaa ccatcatcat
     9841 ctctattacc accctggcgg tcacggtttt aggttccgtg ctgtttcgtg gtttcctggc
     9901 aattatcccg attttaattg gcgtgctggt ggggtacgcg ctctctttcg caatgggaat
     9961 tgtcgatacc acgccgatta ttaatgctca ctggtttgcg ctgccgaccc tctatacgcc
    10021 gcgcttcgag tggtttgcca ttctgactat tctgccagcg gcgttagtgg ttattgccga
    10081 acacgtaggg cacctggtcg taacggctaa tatcgtcaaa aaagatctgc tgcgcgatcc
    10141 aggtctgcac cgttcgatgt ttgctaacgg cttgtcgacc gtgatctccg gtttctttgg
    10201 ttccacacca aacaccactt acggagaaaa cattggcgtg atggcgatca cccgtgttta
    10261 cagtacctgg gttatcggcg gtgcggcgat tttcgctatc ctgctttcct gcgtcggtaa
    10321 actggctgct gctatccaga tgattccatt gccggtgatg ggcggcgttt cgctgctgct
    10381 ttacggggtc atcggtgctt ccggtattcg tgtgttgatc gaatcgaaag tggattacaa
    10441 caaagcacag aacctgatcc tgacttccgt tattttgatc atcggcgtca gtggtgcgaa
    10501 ggtaaacatt ggcgcggcgg agctgaaagg tatggcgctg gcgaccatcg tcggtatcgg
    10561 cttaagtttg atcttcaaac tgattagtat gctgcggcct gaagaagtgg tgctggacgc
    10621 agaagacgcg gatataacag acaaataatg taccgaccgg gcagtgttgc tgcccggttc
    10681 aaacatcatg ggattctgtg gtaaacttct cgcgattttg tgaaatcctg gttgaggtat
    10741 ctctgaacac accggcacag ctctctttgc cactttatct tcctgacgac gaaacctttg
    10801 caagtttctg gccgggggat aactcctctt tactggccgc gctgcaaaac gtgctgcgtc
    10861 aggaacatag cggttacatc tatctctggg cacgcgaagg cgcggggcgc agccatctgc
    10921 tgcacgcggc ttgcgcggaa ttgtcacagc gtggcgatgc ggtgggctat gtcccgctgg
    10981 ataaacgcac ctggtttgtt ccggaagtgc tcgacggtat ggagcatttg tcgctggtct
    11041 gtattgacaa cattgagtgt attgccggcg atgagttgtg ggagatggcg attttcgatc
    11101 tctacaatcg aattctggaa tcgggcaaaa cacggttgtt gatcaccggc gatcgtccac
    11161 cgcggcagtt gaatctggga ttaccggatc tcgcgtcgcg tctcgactgg gggcagatct
    11221 acaaattgca gccactttct gatgaagata agttgcaggc gctacagtta cgcgcgcgtt
    11281 tgcgtggttt tgaactgccg gaagatgtgg ggcgtttctt gctgaagcgg ctcgacagag
    11341 aaatgcgcac gttgtttatg acgctggatc agctggatcg tgcgtcgatt accgcgcaac
    11401 gcaagctgac cattcctttt gtgaaagaaa ttctgaagtt gtagataagg tgtttattgt
    11461 cggatgcggc gcgaacgcct tatccgacct acgcgaagtt aatccatagt attcgtaggc
    11521 cggataagac gcggcaagcg tcgcatccgg caatgtgctt caactagcga tggagcacat
    11581 cttaacccac gatttccagt acctgctccg gtggacgacc aatccgcgct ttgccattcg
    11641 cgaccacaat cgggcgttcc attagcttcg gattctctac catcgcctga atcagcacct
    11701 cttcgctaag cgaactgtca gccaggttca gttctttata aagatcctct ttttggcgca
    11761 tcaattcacg agcgctattc atccccagca ttttcagcaa atcacgcagc gttgccgcat
    11821 cggcgggtgt ctcaaggtag agcaccactt ctggctccac gccgttttct ttcagcaggt
    11881 tcagcgtttc ccggctcttt gaacagcgtg ggttatggta gatttttgcc tgtttggtca
    11941 ttgttcttcc tttaatgcga attacatctt ggtataaggc ttaaagcgtt cctgcagctg
    12001 gcgcaactgg tcgatgcgcg catcgtaacg cgcttgttgc aggctgccta atttcacctg
    12061 cgaactggcg ctactcaaca aggaaatagc ctgatcgagt cgcccggcga gcgcataacc
    12121 ttctgctcgc gcagccagtt cctgatcgtg gttatttagt gcggcttccg cctgtgccag
    12181 taaatcccag ccgttgctgt catctttatt gttaaaagtg tagcgattca gaatattggc
    12241 cgcttcctgt ggttgccccc cttgcagata agcgttcgcc aggttgagct gcaacaccgg
    12301 attggtgcgc aaatcgcggg catttttcag gcgattgata gcgtcattgg ctttgttttg
    12361 cccaagatcg atatcggtag ccagatcgag ataccatgcg ttgccaggtt ctgccgccag
    12421 taacggttgc agcgttttac gcgcctcgtc gtatttattg gcttccatcg cctgtaaagc
    12481 acgaccatat tgcgccgctc gttgctgacg aacgtttcct ttcgcccatt catccagcaa
    12541 atcactggtg agctggttac gtccggaatt atacatcccc agtgtgcgcg ctttcgccag
    12601 atagaaatct tccgacgact gcaccaccat cgggcgcatc tgattagcac ggttgcgggc
    12661 atctgccaga cgactttccg gcaacgggtg agtcaataaa atttccggcg gacgcgaaga
    12721 gtaacgcgcc tgatcgagta atttttcgag gaaggtcggc atcgcctgcg gatcgaatcc
    12781 cgagcgttgc agcacctgaa taccaatgcg gtccgcttcc tgttcatttt gctgggtgaa
    12841 actgatcatc ccctggcgcg ttcccgccag tgtaccggtc agcgccgcca tccccgcctg
    12901 cggactggcc atcgccagta aaatagaacc taacgcgccg acccaggtca gcggcgcgct
    12961 gcgctgctga tcttccatcg ctcgcgccag gtgacgttgg gtgacgtggg agatttcgtg
    13021 cgccataact gaagccagtt gactttcgtt atcggaataa cggaacaggg cagagtgcag
    13081 caccacgttg ccgccaaaga aagcaaaggc gttaatttcg tcgttgttga tcagaaagaa
    13141 atgaaacggt gtcttaaccg aattggcatg cgaaaccaga cgcattccta gcgaattaat
    13201 gtattgcgtt aacagcggat cattaattaa cggcgcgctg ccgcgtaact ggcggacata
    13261 atagtcgccc atctgcattt cctgaccaat ggaaagtgtg cttcctgcgg aggttcccat
    13321 atccggcaag gtgtctgcgc tgtcggcaaa cgccggggct acctgaccaa tggtcatagc
    13381 agcaatgagg gttgcaacca ggtttttttt caactgcctg aacataacct ctatcctgta
    13441 tttctggaga tagtcatttg accgatactg tgaaatatcg ttcccaccgc gccgcctttg
    13501 agtgtaacgg cgaagattag cccactcaaa tgacttttca gtttcctgaa tccgtcataa
    13561 aaagcgaagt cttttttgtg acatttcgat acaattgcgg atcgcaatcc cgctcttgag
    13621 gttcagggaa ggtctgtatg ctcgaaatgt tgatgcaatg gtatcgccgc cgttttagcg
    13681 acccggaagc gattgccttg ctggttattt tggttgctgg ttttggcatt atctttttct
    13741 ttagcggcct actcgcgccg ttgctggtgg ctattgtgct ggcctattta ctggaatggc
    13801 cgactgtccg cctgcagtct attggctgtt cccgcccttg ggcgacatct atcgtgctga
    13861 tcctgtttgt gggcatcctg ctactgatgg cgttcgtggt actgcctatc gcctggcagc
    13921 agggcatcta cttaatccgc gatatgccgg ggatgctcaa taagctttct gactttgccg
    13981 ccacgttgcc gcgccgctat ccggctttaa tggatgcggg cattattgat gcaatggctg
    14041 aaaatatgcg cagtaggatg ctgaccatgg gcgattcggt ggtgaaaatt tccctcgcct
    14101 cgttggtcgg tttgctgacc attgccgtct atctggtgct ggtaccattg atggtcttct
    14161 tcctgctgaa agacaaagag cagatgctga acgccgttcg ccgggtgctg ccgcgcaacc
    14221 gtggactggc aggacaggtg tggaaggaga tgaatcaaca aatcaccaac tatatccgcg
    14281 gcaaagtgct ggagatgatc gtggtaggga tcgccacctg gctggggttc ttgctctttg
    14341 ggctgaacta ttcgctgctg ctggcggtgc tggtcggctt ctcggtactt attccgtaca
    14401 ttggcgcatt tgtggtgacc attccggtgg ttggcgtggc gctattccag tttggtgctg
    14461 gcacggaatt ctggagctgc ttcgcggtgt atctgattat tcaggcgctg gacggcaacc
    14521 tgttagtacc ggtgttgttc tccgaagcgg ttaacctgca tccgctggtg attattttat
    14581 cggtggtgat cttcggtggt ttgtggggat tctggggcgt attcttcgct attccattgg
    14641 cgacgctgat caaagccgtg attcacgcct ggcctgatgg gcaaatcgcg caagactgac
    14701 cttcagcgaa aaaatttgaa acgagtgttg cagggcagtg agaagatgtg agatgtgctg
    14761 gccgcaagcg cagccagcac ggaatggagc aaagtaatca ggcgtgttct ttcagccagt
    14821 tcagcacaac gtcgtggtga ttgctggttt tgaaatcgtc aaagacatgt tcgattttgc
    14881 catcagcgtc aatcaggaag ctgatgcgat gaataccatc gtaggttttg cccatgaagg
    14941 acttttcacc ccagacgccg aattgttcgc acacctggtg gtcctcatca gacaggagcg
    15001 taaagttaag cagctctttt tccgcaaaac gggagagttt ttcgggttta tcggtgctga
    15061 tacccagcac atcaacgccc gcttttttca actcatccat gttatcgcgt aatccgcagg
    15121 cctgtacggt acagccgggg gtcatggctt tcgggtagaa ataaaccaga acacgctgtc
    15181 cctggaagtc ggtcaaatta acttgttctc cgtcttgatc cggcaagcta aatttcggtg
    15241 cgatatcacc ggctttcagt ggattcatta cttaactcca tcctgttcat catgttggga
    15301 ataattgacg acgttaatac tgccttgtgc attgagttct gtacataggg ctttgaacgc
    15361 ttgctcaata tttgctgcgt ccgcagatgc ggggctgtgg gcagttatct gaatatgcaa
    15421 ctgcgcagcc ctttcatttt cagcaggttg cgtgcgcgac accagctccg caatgttcat
    15481 atgatgcgtg tcgaaaagtg ctgtgaagcg ttcaattaaa tgcggggagt ctgccacatc
    15541 gacctgaacc cagacagatg ctggcattgg cggacgcgga cgcgccgtcg tgcgcttcat
    15601 cacgattaaa agatccagct cggcaccttt caacggtaac gttgattcaa tcagagtaat
    15661 ggcattccat gaaccggaaa gcagcatgat aaacgtgaac tcttctccca gcatcgccag
    15721 gcgactgtct tcaatattac agccgcaact actgacatga cgggtgatgg tgttcactat
    15781 tccagggcga tcggcaccca acgcagtgat caccagataa tgttgcgatg acagtgtcaa
    15841 actggttatt ccttcaaggg gtgagttgtt cttaaggaaa gcataaaaaa aacatgcata
    15901 caacaatcag aacggttctg tctgcttgct tttaatgcca taccaaacgt accattgaga
    15961 cacttgtttg cacagaggat ggcccatgtt cacgggaagt attgtcgcga ttgttactcc
    16021 gatggatgaa aaaggtaatg tctgtcgggc tagcttgaaa aaactgattg attatcatgt
    16081 cgccagcggt acttcggcga tcgtttctgt tggcaccact ggcgagtccg ctaccttaaa
    16141 tcatgacgaa catgctgatg tggtgatgat gacgctggag ctggctgacg ggcgcattcc
    16201 ggtgattgcc gggaccggtg ctaacgctac tgcggaagcc attagcctga cgcagcgctt
    16261 caatgacagc ggtatcgtcg gctgcctgac ggtaacccct tactacaatc gtccgtcgca
    16321 agaaggtttg tatcagcatt tcaaagccat cgctgaacat actgacctgc cgcaaattct
    16381 gtataatgtg ccgtcccgta ctggctgcga tctgctcccg gaaacggtgg gccgtctggc
    16441 gaaagtaaaa aatattatcg gaatcaaaga ggcaacaggg aacttaacgc gtgtaaacca
    16501 gatcaaagag ctggtttcag atgattttgt tctgctgagc ggcgatgatg cgagcgcgct
    16561 ggacttcatg caattaggcg gtcatggggt tatttccgtt acggctaacg tcgcagcgcg
    16621 tgatatggcc cagatgtgca aactggcagc agaagggcat tttgccgagg cacgcgttat
    16681 taatcagcgt ctgatgccat tacacaacaa actatttgtc gaacccaatc caatcccggt
    16741 gaaatgggca tgtaaggaac tgggtcttgt ggcgaccgat acgctgcgcc tgccaatgac
    16801 accaatcacc gacagtggtc gtgagacggt cagagcggcg cttaagcatg ccggtttgct
    16861 gtaaagttta gggagatttg atggcttact ctgttcaaaa gtcgcgcctg gcaaaggttg
    16921 cgggtgtttc gcttgtttta ttactcgctg cctgtagttc tgactcacgc tataagcgtc
    16981 aggtcagtgg tgatgaagcc tacctggaag cggcaccgct tgcggagctt catgccccgg
    17041 ctggaatgat cttgccggtg acctccggcg attatgcaat cccggtgacc aacggtagtg
    17101 gtgccgtcgg taaggcgctg gacattcgtc caccagccca gccgctggca ctggtttctg
    17161 gcgcgcgtac ccagttcacg ggcgataccg cttcattgct ggtggaaaat ggtcgtggca
    17221 atactctgtg gccgcaggtg gttagcgtgc tgcaggcgaa aaactacacc atcacccaac
    17281 gtgatgatgc tggtcagaca ctgaccaccg attgggtaca atggaaccgt ctggacgaag
    17341 acgagcagta tcgtggtcgt tatcaaatct ctgttaagcc gcagggttat cagcaggcgg
    17401 ttacggttaa actgctgaac ctggaacagg cgggcaaacc ggttgcagac gcggcttcca
    17461 tgcagcgtta cagcacggag atgatgaacg ttatttccgc cggtctggat aaatctgcca
    17521 ctgacgccgc gaacgctgcg caaaatcgtg cctccaccac tatggacgta caaagtgcag
    17581 ctgatgacac cggtttacca atgctggtcg tacgcgggcc gttcaatgtg gtttggcaac
    17641 gtctgccagc tgcgctggaa aaagtgggca tgaaagtgac cgacagcacc cgttcgcagg
    17701 gcaacatggc cgtaacttat aagccgctgt ctgacagcga atggcacgaa ctgggcgcga
    17761 gcgatccagg tctggcatcc ggtgactata aactgcaggt tggcgattta gataaccgca
    17821 gcagcctaca gttcatcgat ccgaaaggtc atactctgac tcagagtcag aacgacgcgc
    17881 tggtagctgt cttccaggct gcgtttagca agtaaaaata cagggctgga gtcatccggc
    17941 ccttttttct gatatgatac gcaaacgtgt gcgtcgacag aaaaacgcga ttttagcggt
    18001 aattcgcacg aaatttgttt gtcggacgta ggtcggataa ggcgtttacg ccgcatccga
    18061 caaaacatcc ggcacaccag acagcaaaag attttaaaac gttaattcac acccaggagt
    18121 gataaagatg caaaagcaag ctgagttgta tcgtggtaaa gcgaaaaccg tatacagcac
    18181 ggaaaacccg gacctgttgg tgctcgaatt ccgcaatgat acgtcagcag gggatggcgc
    18241 gcgcattgag cagtttgatc gcaaaggtat ggtgaacaac aagttcaact acttcattat
    18301 gagcaaactg gctgaagcgg gtatcccgac tcaaatggag cgtctgctct ccgataccga
    18361 atgtctggtg aaaaagctgg atatggtgcc ggttgagtgt gtcgtgcgta accgtgctgc
    18421 tggctctctg gtgaaacgtc ttggaatcga agaaggtatt gagctgaacc cgccactgtt
    18481 cgatctgttc ctgaaaaacg acgccatgca cgatccgatg gtcaacgaat cttactgcga
    18541 aacctttggc tgggtgagca aagagaacct ggcgcgtatg aaagagctga cctacaaagc
    18601 gaacgacgtg ctgaaaaaac agttcgatga tgctggtctc attctggtcg acttcaagct
    18661 ggagtttggc ctgtacaaag gtgaagtggt actgggcgat gagttctccc cggacggcag
    18721 ccgcctgtgg gacaaagaaa cgctggagaa aatggataaa gaccgtttcc gccagagcct
    18781 cggtggcctg atcgaagcct atgaagccgt tgcccgccgc ctgggtgtac agctggactg
    18841 atttttctgt tcatcatctt gccgtgctgc tggcacggca agacaaccgc tctcataaga
    18901 tgtgcattga acgctcattc ctccgccatt tcatcccgct ttcctgtggt aatcttcctc
    18961 tgaaccacct acgatcattt ctataatgaa tataatgttg aggtatctat gcgttggcaa
    19021 ggacgacgtg aaagtgacaa tgttgaagac aggcgcaata gctccggcgg tccgtcaatg
    19081 ggcggtcccg gttttcgcct gccaagcggt aaaggcgggc tgattttact gatagtcgtg
    19141 ctggttgcag gctactatgg cgttgattta accgggttga tgaccggaca gccggtttcc
    19201 caacagcaat caacgcggtc aattagccct aatgaagacg aagccgcaaa attcacctcg
    19261 gtgattctgg caaccacgga agacacctgg ggacagcaat tcgagaagat gggtaaaacc
    19321 tatcagcaac cgaagctggt catgtaccgt ggaatgacgc gtaccggctg cggggcgggc
    19381 cagtccataa tggggccatt ctattgcccg gcggatggca cagtttatat cgatctctcc
    19441 ttctatgatg acatgaaaga caaacttggc gcggatggcg attttgccca ggggtacgtt
    19501 atcgctcatg aagtcggtca tcatgtgcag aaactgttag gcatcgagcc gaaagttcgt
    19561 caactgcaac aaaacgcgac gcaggcggaa gtaaaccgct tatctgtgcg tatggaactc
    19621 caggccgact gttttgccgg tgtctggggg catagtatgc agcagcaagg tgttctggaa
    19681 accggcgatc tggaagaggc gctgaacgcg gcgcaggcca tcggcgatga ccgtttacaa
    19741 cagcaaagtc aggggcgagt agtaccagac agtttcactc atggcacttc tcagcaacgc
    19801 tacagctggt ttaaacgtgg tttcgacagc ggcgatccgg cacaatgcaa tacttttggt
    19861 aaaagcattt aacttccggg gcagggatgg ctgaactgac tgcgcttcac acattaacag
    19921 cgcaaatgaa acgtgaaggg atccgccgct tgctggtgtt gagcggggaa gagcgttggt
    19981 gttttgatca tgcgcttaag ttgcgtgatg ccttacctgg cgactggctg tggatttcgc
    20041 cgcagccaga tgctgaaaac cactgttctc cctcggcact acaaacttta cttgggcgcg
    20101 agttccggca tgcggtattc gacgcccgcc aaggctttga tgccgctgcc tttgcggcac
    20161 ttagcggaac gttgaaagcg ggaagctggc tggtgttgtt actccctgta tgggatgagt
    20221 gggaaaacca acctgatgcc gactcgctgc gctggagtga ttgccctgac cctattgcga
    20281 cgccgcattt tgtccagcat ttcaaacgcg tacttacggc gaataacgac gctatcctct
    20341 ggcggcaaaa ccagccgttc tcgttggcgc attttactcc ccgtactgac tggcaccccg
    20401 ctaccggcgc accacaacca gaacaacagc aactcttaca gcagctactg accatgccgc
    20461 cgggcgtggc agcggtaact gctgcgcgtg ggcgcggtaa gtcggccttg gcagggcaac
    20521 tcatttctcg tattgcgggc agtgcgattg tcaccgcgcc cgcaaaagcg gcaacggatg
    20581 tactggcaca atttgcgggc gagaagtttc gctttattgc accggatgcc ttgttagcca
    20641 gcgatgagca agccgattgg ctggtggtcg atgaagccgc agctatacct gcgccgttgt
    20701 tgcatcaact ggtatcgcgt tttcctcgaa cgttgttaac cactacggtg cagggctacg
    20761 aaggcaccgg acgtggtttt ttgctgaaat tttgcgctcg ctttccgcat ttacaccgtt
    20821 ttgaactaca acagccgatc cgttgggcgc agggatgccc gctggaaaaa atggttagtg
    20881 aggcactggt gtttgacgat gaaaacttca cccatacacc acaaggcaat atcgttattt
    20941 ccgcatttga acagacgtta tggcgaagcg agccagaaac gccgttaaag gtttatcaat
    21001 tattgtctgg tgcgcactac cggacttcgc cgctggattt acgccgcatg atggatgcac
    21061 caggtcaaca ttttttacag gcggctggcg aaaacgagat tgccggagcg ctgtgtctgg
    21121 tggatgaggg tggattatct caagaactca gtcaggcggt atgggcgggt tatcgtcgtc
    21181 cacggggcaa tctggtggct caatcgctgg cggcgcacgg cagcaatcca ctggcggcga
    21241 cattgcgcgg acggcgggtt agccggatag cagttcatcc ggctcgtcag cgggaaggca
    21301 cagggcgaca gcttattgct ggtgctttgc aatatattca tgacctcgac tatctttcgg
    21361 tgagttttgg ttacaccgag gagttatggc gtttctggca acgctgcggt tttgtgctgg
    21421 tgcgaatggg taatcatcgt gaagccagca gcggttgcta tacggcgatg gcgctgttac
    21481 cgatgagtga tgcgggtaaa caactggctg aacgtgagca ttaccgttta cgtcgcgatg
    21541 cgcaagctct cgcgcagtgg aatggcgaaa tgcttcctgt tgatccacta aacgatgccg
    21601 tcctttctga cgacgactgg cttgaactgg ccggttttgc tttcactcat cgtccgctat
    21661 taacatcgtt aggttgctta ttgcgtttgc tacaaaccag cgaactggca ttaccggcgc
    21721 tgcgtgggcg tttacagaaa aacgccagtg acgcgcagtt atgtaccaca cttaaacttt
    21781 caggccgtaa gctgttactg gtccgtcaac gcgaagaagc cgcgcaggcg ctattcgcac
    21841 ttgatgatgt ttgcactgag cgtctgcgcg atcgcataac gcaatggcaa ttttttcact
    21901 gactccttca attttctccc atggtcattg tgagtcagcg gcgtaaaatt aatgacatca
    21961 gttaaggagt tcgccatgaa acatgaccat tttgttgttc aaagcccgga taaaccagca
    22021 caacagttgt tactgctttt ccacggtgtc ggggataacc cggtggcaat gggggaaata
    22081 ggtagctggt ttgcgccgct gtttcctgat gcgctggtag tgagtgtcgg tggcgcggag
    22141 ccgagcggta atccggcggg gcgtcagtgg ttttcggtgc agggtattac ggaagataat
    22201 cgccaggcgc gtgtggatgc gatcatgccg acgtttattg agacggtgcg ctactggcag
    22261 aaacaaagcg gagtaggggc taatgccacg gcgctaatcg gtttctcaca aggcgcgatt
    22321 atggcgctgg agagcattaa agccgaaccg ggccttgctt cgcgcgtcat tgccttcaac
    22381 ggacgctacg cgagcctgcc ggaaacagcg tctacagcca caacaattca cttgattcat
    22441 ggcggtgaag atccggttat cgatctggcg catgcggttg cggcgcagga agcgttaatc
    22501 agtgccggtg gcgatgtgac gctggatatc gtggaggatt taggccatgc gatcgacaat
    22561 cgcagtatgc aattagccct ggatcacttg cgttatacca ttccgaaaca ttatttcgac
    22621 gaagcgctaa gtggcggtaa gcctggcgat gatgatgtga ttgaaatgat gtaataatag
    22681 ccgggtaggc ctgataagac cgtatcaggc atcatggccg gatgcggcgt taacgcctta
    22741 tccggccact tagcttactt cttcggttga tcctttttcg gccagtcgtc gtcatcgtcc
    22801 catttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag aaatttctta
    22861 tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctactaa aaagacaatc
    22921 accagaatcc accaatattt agccagccag tccatgctta tttcctcttg cagaaccact
    22981 catcaggcga cgagctgttc catgatacgt tgatacatac gggcaagtag ctgcaggtcg
    23041 gcagcgttca cacattcatt aattttatga atcgtggcat tgaccggccc gagttccacc
    23101 acctgcgccc ccatgcgggc aataaagcgc ccgtcggacg ttccgcctgt ggtcagtagc
    23161 tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac cagtttaccg
    23221 cgcgcggtca aaaatggctg cccggaaagc caccaatcca ccgtatagcg cagttgatgt
    23281 ttttcaagca gggcaagtac ctgcgctttg atcatctcat cagtcagttc ggtgctgaag
    23341 cggaagttaa actgcacaaa cagttcaccc ggaataacgt tgttactgcc cgttcccgcc
    23401 tgaatattgg caatctgcat actggtcgcc gggaagaatt cattgccctg atcccactca
    23461 atagccacta attcattaag gaaaggtgct gcgcgatgta ccggattgtc agccagatgt
    23521 gggtaggcaa catgcccctg cacgccatga atagtaaggt tacaggttaa cgatccgcga
    23581 cgaccatttt tcaccacatc accaaccact tcgatactcg acggttcgcc gaccaggcag
    23641 taatcgagac gctcattacg cgccattaac gcttcgacga cttttaccgt accgttgtgg
    23701 gcactggctt cttcatcaga ggtgatcaga aatgccagtc gtcccgtatg gttgggatgt
    23761 tgtgcgacaa agcgttctgc cgctaccacc atcgccgcca gcgagccttt catatctgcc
    23821 gcaccgcgcc cgaataacat gccatcacga atggtgggtt caaacggcgg attgatccaa
    23881 cggtcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggctaa cgtttcaccc
    23941 tgcccacgcc atgcccaaaa attctgcgtg tcggcaaagt ccatgcgttc aacggtaaaa
    24001 ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc ctgcgtcatc aggactcagg
    24061 gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat agactacacc
    24121 tcatggaaaa attgctgata actggattca ctgaaaccca gcagcatagg cttaccaggc
    24181 gcgcagagca atggacgttt gataattgca ggcatttcag tcattaatgt cgccgcagag
    24241 gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt tacgccaggt tgtaccacgg
    24301 gtgttgagta acgcttccca gcctaattcg ttgataaaac cgttcaataa ttcgctgtcc
    24361 aatccatcga cgcggtaatc atgaaaacga tagtcgatgt tattagcttc cagccaacgg
    24421 cgagccttct taatggtgtc acaatttttg atgccgtaaa gtgtaaccat aatgaatcct
    24481 ttttcgattt atgagaataa atactcattt aaaggtgaaa acattttgcc tgcaaatgat
    24541 tatcaggaaa tataacatta tcgaagtatt caaattaaca accattaaat aacattaaac
    24601 atttttaacg taaacattaa gaatattcat cttgttatct gtctgtattt taaccagaat
    24661 taaatcagta ttagatgccg tatgaatatc tcgaaatgtt gcggtgcatc acatacaaat
    24721 catcaccgca ggaccatagt atttacataa ggtttgtccc ccggaggtag tcatcacagc
    24781 aattgagtaa attagcttca ccacggtata ttgtttccgt agaataccca taattataag
    24841 agaggttgtt atgattgaac gtgaactggg gaactggaaa gactttatcg aagttatgct
    24901 tcgtaagtaa ttcctgagac aaagtacaaa cagcaagaat ccgctgagca ggttcttaat
    24961 cggaaagttg aaaagggcga cacattggca tgtcgccttt tttattgctt attccgggcg
    25021 cggcttcagc gggaagcggc ggcgcaccag cacaaagaac agcggcacga agtaaatagc
    25081 cagaatagtg gccgaaatca tcccgcccat tacgccagta cccaccgcat gctgaccacc
    25141 ggaaccggca cctgtgctgg ttgccattgg caatacgccg aagataaatg ccagcgaggt
    25201 catcagaatc gggcgtaaac gctgacggca ggcgtggagc gtcgcttcaa acaggtcgtg
    25261 gcctttttgg ttcatctcgt tagcaaactc gacgatcagg atagcgtttt tcgccgataa
    25321 accaatgacc gttaacaggc ccacctggaa gtaaacatcg ttttccagcc cgcgcatcca
    25381 ggttgccagc agcgcgccga taacccccag cggcacgacc agcattaccg agaacggcac
    25441 cgaccagctt tcatacagcg cagccagaca caggaatacc accagcaagg aaatggcgta
    25501 cagcgccgga gcctgcgcgc cggaaagccg ctcctgatac gacatcgccg tccactccag
    25561 accaaagccg tttggcaact gcttcactaa cgattccata atatccatcg ccgtaccggt
    25621 actgaccccc ggtgcggctt ccccaacaat ctcaaccgca gaataaccgt tatagcgttc
    25681 cagacgcggc gagcctgtct cccagcgtga ggtcgcgaaa gcagagaagg gcaccatgcc
    25741 gccatcttta tttcggacat accagagatt gatgtcatct ggcagcatgc gatacggcgc
    25801 agctgcctgc acatagactt tcttcacgcg accgcgatcc cacatagctc gaaccccagg
    25861 cggtttgcag tgtgtcgtta atatcgtcga tagcaacgcc cagcgcctga gctttacgct
    25921 ggtcgatatc aatctgcaac tgcggactgt cgtcgaggcc gttatggcgc acacgggtta
    25981 gctccgggtt ttccgctgcc agcgccagca actggtttcg tgctgccatc agcgcatcgt
    26041 gacccgctcc agcgtggtcc tgcaactcca tatcaaaacc cgcagaacta ccaagaccgc
    26101 taattgctgg cgggctgctg gcgataacgc gagcttcttt aatcttgtta aacgccttcg
    26161 ttgcacgctc gataatggca aacgaggtgc cggtcttact gtcgcgttcg ctccagtctt
    26221 tcaggcggat aaacattcgc gccacgtttt gcccgttacc cccagggcca gaacccacgg
    26281 tggcaaacac cgacatgatg ttgtcttttt catgggtgaa gtaatatttc tcgatttgct
    26341 caacgacttt cagggtctgt tgctgggttg aaccgctggg caactgtacc gaggtagtaa
    26401 acatgccacg gtcttccagc ggtaagaacg acgtcggcaa acgcaggaac aggaacacca
    26461 tgccgccaag cagcaggaca taaatcacaa tccagcgcag gctacggtgg agaattttcg
    26521 ccaccccttt ttcgtagcgt tcggagttgc ggttaaacat ctggttaaac caggcaaaaa
    26581 agcctttttg cccgtgatgc tcaccttttt tcagtggctt aagtagcgtg gcacacagag
    26641 caggagtgag gatcatcgct accagtactg acagcaccat cgccgcaaca atggtaatag
    26701 agaactggcg atagatggca ccggtggtgc cgccgaagaa ggccattggc acaaataccg
    26761 ccgaaagcac catcgcaatc ccgaccagtg ccccctggat ctgccccatc gatttacgtg
    26821 tggcttcgcg aggagtgagt ccttcctcac tcataatacg ttcgacattt tccaccacca
    26881 cgatggcatc atccaccagc agaccgatcg ccagcaccat cgcgaacatg gttaaggtgt
    26941 tgacactgta accgaaggcg taaagcacgg agaaggttcc catcaacacc accggcacgg
    27001 cgatagtggg tatcagcgtg gcgcggaagt tttgcaggaa cagatacata acgaggaaaa
    27061 ccagggcgat agcttccaga agcgttttca ccacgtcttc aatggaggct ttaacaaacg
    27121 aggtggtttc atacgccacc ttgtattcca gtccgtgtgg aaaatactgc gccagctcgt
    27181 cgagacgatt gagcaccagc tccgctgtcg ccatttcgtt cgcgccggag gccagtttaa
    27241 cccccagccc ggaagctggc ttaccattga agcggctaag ataatcgtat ttctccgccc
    27301 ccatttcgac ggtggcgaca tcgcccagcc ttacctctga gccgtcctga ttcacccgca
    27361 aggtgatatc gcggaactgt tctggtgttt gcagcagtga ctgggcgtta atggtggcgt
    27421 tgagcgcctg cttatcgacg gaaggcgtac caccaagttg cccaaccgca atctgcgcgt
    27481 tctgtgattc aatggcatcg gtgacatctt tagccgtcat ctggaaactg ttgagtttcg
    27541 ccgggtccag ccagatacgc atggaatatt gcgaaccata ggcatcgata tccccgacgc
    27601 cattcacgcg gcttaacggg tcctgaatat tactggcaac ataatcggca atatcctgtt
    27661 tatccatcga accatcggta gagacgaagg caatggtcag aatgttggta tcgccggttt
    27721 tacgcaccgt cacgccctga ttttgcaccg cctgcggtaa ctttcgcatg gctgattgca
    27781 gctggttttg tacttgctgc acggcttcgt ccggatcggt gcctgcttta aaacttaacg
    27841 tgacagatgc ctgaccggtg ccgctgctct gagatgacat atacatcaga ttatcgaggc
    27901 cggtcatatt ttgctcgata acctgggtca cggtgttttc cagcgtctgg gccgatgcgc
    27961 cgggatagtt agcggtcact cgcacattcg gtggcgcgag atcggggtat tgttcaacgg
    28021 gcaatgaaaa aatcgccagg gtacctgtca gacacaacag gattgccagc acccaggcaa
    28081 aaatggggcg atcaataaag aaattcgcca ttaaaagagg acctcgtgtt tctgcgtatc
    28141 gttgtaggct tggcttgctt cactgtagcg gcaaggtacg agccaatcgt ggagaaaaaa
    28201 aggagttaat gtaaattatc atgccctttt atactgaacc ttaagtgcca ctattctttg
    28261 gtcaataaga ggcattacat cagctgactt tcctcaccct ccgcagagcg aaagctaata
    28321 ctcactaacg tgccgccgcc ggaaggttgt gaaaaagtca gcgtcccacc caatctttcc
    28381 gcgcgttcgc gcatgatatt cagaccataa tggccttcgg gttctttcgg ttcaccgatg
    28441 ccaatcccgt tgtcgcgaat atagactgtg tgattgccat ccggcgcggt gacgcagcta
    28501 acggcgattt cgctggcgtt ggcgtgcttc atcgcattca gcaccgcttc gcgaataatt
    28561 tgcaacaaat gcacctgcat ttgcgcatcc agcgccaggg ttggcagacg gcagtcgagg
    28621 gtcagtttgg cgctggtttg attttgtaac gtatccagca tttccctcaa cgcggagggg
    28681 agatccgcct gctgcagcgt caggcggaaa gtagtcaaca gctcgcgtaa ctgccgataa
    28741 gcatcattca acgcctggga aaaatcggcc atgatacttt gtgcggtggc gttatcttcc
    28801 ggtatcgaac gcttcagtaa cgtcaactgg atacgtaagt aagaaagtac ctgagccagc
    28861 gagtcgtgca attcgcgggc gatggtcgca cgttcttcca tcaacaataa ctgctgaaaa
    28921 tgcttctgcg cctgattaaa gtacaaaccg cgtcccagca tcgacgaaac gctgttaagc
    28981 agcggttctg atgatgaaac gtgactattt tgccagtgca gttcgccgta aaccgtctct
    29041 tgcattgtca ccggtaaaat ctgcatcggc aattccgggt ttggctgccc ttcgctaatc
    29101 cgccagttgt caccgacatt taactccaga tattcagccg cttcattgtc gcgaacaatc
    29161 tgcaaaatat ggcggaaaca atgcacatca atctggctgg tgtttagcgc ctgcgaacac
    29221 tgatacaaca cctccagacg acgcttggcc tcgtggagat cgcgggtctt ttcttctact
    29281 gacgcctcca gcgaacggta caatttatgc agctcgctcg acatctggtt aaaggttttt
    29341 gcaagcaggc caagctcatt cggcaggctg gtatccagcg gcggcgagtc gaactgcccg
    29401 tgttcaatac gctgactggc ggtaaccagc tgattcagcg gggcaaccac ctgatggcgt
    29461 atgcggcgca aagtaaaaaa gaccagcgtg aaaataccga tgccgccagc cagggaaatt
    29521 gccaccacca gcagcatttt gcgttcagcg tagtgctgta aagccagcac gaacaggtct
    29581 atctgattaa cataattatt aatattggcc tgataccacg gcaagtcgcc cttgctgagc
    29641 cgattattca tctccagcca attggcattc agatgcgcat agcgggtttt tactgcctct
    29701 ggcacatacc agacgttgag attggttaat accggtgaat gcagtgcctg ctgaaataac
    29761 tggcgatgtg cattgagttg tggactgcca ctttgcaagt cgtagcccag gcggtaactc
    29821 tgcatacgca gcgatccggc aatattgata gcctcagcgt cgcgtaaact gctcgccagc
    29881 gttagcagag cgatacccgt ggaaagaatc gacagcagca caatgtaaaa aaaggcccgg
    29941 gccagactgg ccgagacggg tcgtttaaca atcacacgcg tcttctccac aaaaaatcat
    30001 taatgttcca gttcgctgaa acgaaattta gtcagtgatg ggattatttg atctgccaca
    30061 gtttatagcg aaatagttac ttattctggg cacatgaaca ttgccgattc ccctgtaagt
    30121 cgggtaataa caacactcat ataaagaata aggtttttac aaccaaaaaa gaaggtcgtt
    30181 atgaatcgtt ttattatggc caacagtcag caatgtctgg gttgtcatgc ttgtgaaatc
    30241 gcctgtgtca tggctcacaa tgacgagcaa catgtcctga gccaacacca ttttcatccc
    30301 cgaattacgg ttatcaaaca tcagcagcaa cgtagtgcag tgacctgtca ccattgtgaa
    30361 gatgcgccct gcgcccgtag ctgccctaat ggcgcaatca gccacgttga tgacagcatt
    30421 caggtcaatc agcaaaagtg tattggctgt aaatcctgcg tggtggcctg tccatttggt
    30481 acgatgcaaa tcgttctgac acccgttgcg gtaggaaaag taaaagccac ggcgcataaa
    30541 tgcgaccttt gtgcagggcg cgaaaacggt cctgcctgtg tcgaaaattg cccggcggac
    30601 gcgctgcaac tggtcactga cgccgcactc tccggcatgg cgaaatcccg ccgcttgcgc
    30661 accgcacgtc aggaacaaca accgtggcat gccagtaccg cggcgcaaga aatgccggta
    30721 atgagtaaag tcgagcaaat gcaggcaacg cccgcgcgtg gcgagccaga taaactggcg
    30781 attgaagcgc gcaaaaccgg ttttgatgaa atttatctgc catttcgtgc cgatcaggca
    30841 caacgggaag cctcgcgctg ccttaagtgc ggcgagcaca gcgtttgtga atggacctgc
    30901 ccgctgcata accatattcc gcagtggatt gaactggtga aagccggaaa catcgacgcc
    30961 gccgtcgagc tttctcacca gaccaacacc ctgccggaaa ttaccggacg cgtttgcccg
    31021 caagaccgtt tgtgtgaagg tgcctgtact attcgcgatg agcacggcgc agtaactatc
    31081 ggcaacattg aacgctacat ttcagatcag gcgttggcga aaggttggcg tcctgactta
    31141 agccatgtca ccaaagtgga caagcgggtg gcgattatcg gtgcgggtcc ggcagggctg
    31201 gcctgtgcag atgttctgac ccgcaatggc atggcggtga cggtgtacga tcgccatcca
    31261 gaaatcggtg gcttgctcac tttcggcatt ccttctttca aactggataa atccctgctg
    31321 gcacgccgtc gggaaatctt cagcgcgatg gggattcact tcgaactcaa ttgtgaagtg
    31381 ggtaaagatg tttctttgaa ttcgcttttg gaacaatacg acgcggtctt cgttggcgta
    31441 ggcacttacc gttccatgaa agcgggttta cccaatgaag atgcgccggg cgtttatgac
    31501 gcgctgccgt tcctcatcgc caacactaaa caggtgatgg gactcgaaga gctaccggaa
    31561 gagccgttta tcaataccgc cggacttaac gtcgtggtac tgggcggcgg cgacaccgcg
    31621 atggactgtg tgcgtaccgc gctgcgccat ggcgcgagta atgtcacctg cgcttatcgt
    31681 cgtgatgaag ctaacatgcc aggctcgaag aaagaggtga agaacgcccg tgaagagggg
    31741 gccaacttcg aatttaacgt ccagccggtg gcgcttgagc tgaatgaaca aggtcacgtc
    31801 tgcgggattc gtttcctgcg cacgcgtctt ggcgagccgg atgcccaggg gcgtcggcgt
    31861 ccagtgccgg tggaaggcag tgaatttgtc atgccagccg atgcggtgat tatggcgttt
    31921 ggcttcaatc cgcacggaat gccgtggctg gagtcgcacg gtgtaacggt agacaaatgg
    31981 ggccgcatca tcgcggatgt ggaaagccag taccgttacc agaccacgaa tccgaaaatc
    32041 ttcgccggtg gtgacgccgt gcgtggtgcg gatctggtgg ttaccgcaat ggcagaagga
    32101 cgtcatgcgg cacaggggat cattgactgg ctgggcgtaa aatcagtcaa atctcactga
    32161 tagcctgcgc agacaaaccc gacttcacag cgtaagataa ttgttcattt cgcgctgtgg
    32221 agtcggtatg acgcaacaaa tcaccctcgt taaagacaaa attctctccg ataactattt
    32281 caccctgcac aacattactt acgatctcac gcgcaaagat ggcgaagtta tccgccataa
    32341 acgtgaagtt tacgatcgcg gtaatggtgc gacgatcctc ctgtacaacg cgaagaaaaa
    32401 gaccgtggtt ctgattcgcc agttccgcgt cgctacctgg gttaatggca atgaaagcgg
    32461 gcaactgatt gaaacctgcg ccgggctgct ggataacgac gaaccggaag tgtgcattcg
    32521 caaagaagcg attgaagaga cgggctatga agttggcgaa gtccgcaaat tatttgaact
    32581 gtatatgtcg ccaggcggtg tgactgagct aatccacttt tttatcgccg aatacagtga
    32641 cagccagcgc gctaacgccg gtggtggtgt cgaagatgaa gatattgaag tgcttgagct
    32701 gccgttcagt caggcgctgg atatgattaa aaccggcgag atacgtgacg gtaagacggt
    32761 gttattgctt aactatttgc aaatgtcaca tttaatggac tgaaaaataa caataatatt
    32821 tcgttgttta ttattggctc aatccgacaa atccgattga gccgtgctac ctgcgcaacg
    32881 aagatacgac ttgtgctgtt tgtttgaact tctggggtcg taccgtccat gcgatatcgc
    32941 attttccttc tctttttttt cgctttgttg ccgatgtctt tggtgtgggc ggcaccagcg
    33001 caacgggcgt tttccgactg gcaggtcacc tgtaataatc aaaatttctg tgtagcgcgc
    33061 aatacgggcg atcataatgg actggtgatg accctgagcc gcagcgccgg ggcgcatacc
    33121 gacgccgttt tacgtattga gcgcggcggg ttgaaatcac cggatgtgtc ggaaggggaa
    33181 atagcgccac ggctgctgtt agatggtgag ccgttggcgt taagtggtga caagtggcgg
    33241 atttcaccat ggttattagt aaccgatgat acggcaacca ttaccgcgtt tttgcagatg
    33301 attcaggaag ggaaggcaat caccttacgc gatggcgatc agaccatttc tttgaatggc
    33361 ttaaaagcgg cgttgttgtt tattgatgcc cagcaaaagc gtgttggcag tgaaaccgcg
    33421 tggataaaga aaggggacga gccgccgctc agcgtaccgc ccgcgcctgc gctgaaagag
    33481 gtcgtggtgg ttaacccaac gccgacgcca ctctcacgcg aagaacgtaa cgatttgctg
    33541 gattatggta actggcggat gaatggtctg cgctgctcgc ttgatccgtt gcgtcgtgaa
    33601 gtgagcgtta cggcactgac cgatgataaa gcgctgatga tgattagctg tgaagcaggc
    33661 gcgtataaca ccattgacct ggcgtggatt gtgtcgcgta aaaaaccgct ggcctcgcgc
    33721 ccggtacggt tgcgtttgcc gttcaacaac ggtcaggaga ccaatgaact ggaactgatg
    33781 aacgcaacat tcgatgaaaa atcgcgtgaa ctggtgacct tagcaaaagg gcgcggatta
    33841 agcgattgtg gcattcaggc gcgctggcgc tttgacggtc agcgttttcg cctggtgcgt
    33901 tacgccgcag aacccacctg cgataactgg catgggccag acgcctggcc cacgttgtgg
    33961 atcacccgtt aaacaaaatg gcggggacat gtagaccgga taagactcac cagcgtcaca
    34021 tccgacaatc agcatccggc gatcaccatc aggcaccttt cactcccagt atcttatgcg
    34081 cttttgccac aatattctcg gcggtaaagc caaagaacgg gaacagctta tccgccggag
    34141 cagattcccc ataacctgtc atcccgacaa ttgccccttt cagaccaaca tatttgtacc
    34201 agtaatcggc aatacccgct tctactgcca cgcgggcaga aacatttgaa ggcagtaccg
    34261 actcgcgata ttcctcatcc tgggcatcga aaatatcggt cgagggcagg gaaaccacgc
    34321 gaacattgcg accttctccc gctaatttct ctgccgcttg cagggtgatt tccatctctg
    34381 aaccggtggc aatcagaata atatctggct taccgccgct gtctttcagc acataaccgc
    34441 cacgagcaat ctctttaacc tgatccggtg ttcgttctac ctgtgccaga ttctgccttg
    34501 agaggatcag tgctgtcggt ccgttgtggc gctcaaccgc cagcttccag ccaaccgccg
    34561 cttccacctg atcgcacggt cgccaggtgc tgaaatttgg cgttaagcgc agactagcca
    34621 gttgctcaac agcctggtgc gtcggaccat cttcgcccag accaattgag tcgtgggtat
    34681 aaaccataat ctgccgcgct ttcatcagtg ccgccatccg cgcggcgtta cgggcgtatt
    34741 caacaaacat caggaacgtc gcggtatacg gcacaaagcc gccgtggtgc gcgatgccgt
    34801 tggcgatagc ggtcatacca aattcacgca ccccgtagtg aatgtagttg cccgccggat
    34861 cttccttcag cgaaacagaa cctttccaga tggtcaggtt gctgggagcc agatccgccg
    34921 aaccgccgag caactcaggc agcatcggcc cgtaagcgtt aagcgtattt tgcgaagcct
    34981 tacgggtagc gattttcgcc ggattcgcct gcaactcatt gatatatttc tgagtcgttt
    35041 tctcccagtc cttcggcaaa ccgccgctca tccgacgggt aaactcttct gccagttgcg
    35101 gatgagcctt tttataggcg gcaaacttct cattccagcg ctgctgcgct ttttcgcctt
    35161 tttcgcgggc attccaggcg tgataaatct ctttagggat ctcaaatggc ggatggtgcc
    35221 agcccagttt ttgccgtgcc agcgccactt ctttttcccc cagtggtgcg ccgtgcgcct
    35281 cttccttacc tgctttattc ggcgaaccaa agccaatcac cgtacggcag ataatcagcg
    35341 acggcttatc tttcacgctt tgcgcttcaa ggatcgcttc cttcaccgcc tgcggatcgt
    35401 ggccgtcgat ctcgtggacc acatgccagt gataggcttc aaatcgtttt gccgtatcgt
    35461 cggtaaacca gccttcggtt tcaccgtcga tggaaatacc gttgtgatcg taaaaaccaa
    35521 tcagcttgcc cagccccagc gtgcccgcca gcgaacagac ttcgtgagag ataccttcca
    35581 tcaggcagcc gtcgcccata aacacatagg tgaagtgatc gacaatttca tgatccggct
    35641 gattaaactg cgccgccagc gtacgctctg ctatcgccag cccgacggcg ttcgccaaac
    35701 cttgtccaag aggaccggtg gtggtttcaa cacctggcgt atagccgatt tccgggtgac
    35761 caggggtttt cgaatgcaac tgacggaagt tcttcagttc ttccagtggc aggtcgtaac
    35821 cggtcagatg cagcaaactg tagagcagca tcgacgcgtg accgttggaa agaataaagc
    35881 ggtcacggtc ataccaggtt gggtcggtgg ggttatgttt aagaaaatcg ttccacagca
    35941 cttcggcaat atcagccatg cccatcggcg cgccgggatg accagaattg gctttttgta
    36001 ccgcatccat actgagtgcg cgaatcgcat tggcaaggtc ttttcgggac atataacact
    36061 ccgtggctgg tttatagttt ggcggcaaga agatcttcca gtttgcgttg atcaacggcg
    36121 aacagacgaa tgccttcaga cagtttttct accgccatcg catcctgatt gtgctcccaa
    36181 cggaattccg cttcgctcat gggagctggg cgtgggaacg tctgagaagg tgggattaat
    36241 ttacgtacca ctggcgaaac tttttcctgt agctccttca gcaaattcgg tgcgatagtc
    36301 agtcggtcgc agccggttaa ggcgaggatt tgttcggtgc gacggaagct cgcgcccatc
    36361 acaatggttt catagtggtg ttgcttatag tagtcgtaga tattgcgtac tgatttaacg
    36421 cctggatctt cttccaccac atacgggtct atcggcttgc gtgcctgata ccagtcataa
    36481 atacgcccga caaacggcga aatcagaaaa acgcctgctt ctgcacaggc ccgcgcctgt
    36541 gcaaaagaaa acagcagcgt caggttgcag ttaatacctt ctttttccag ctcttctgcc
    36601 gcgcgaattc cttcccaggt cgaagccagc ttgatcagaa tgcgtgattt ctcaacgcct
    36661 tgttgctgat acaagtccac cagatggcgc gccttctcaa tactcttttc tttatcaaaa
    36721 gagaggcgtg catcaacttc tgttgacacg cgaccgggta cgattttgag aatttcagca
    36781 ccgaaattga ccgccagttt gtcacacgct gcgaccacct gttgttcctg ggtcttgcca
    36841 ttttttttac cccaggcgat agcatcgtct attaaatgct catattgtga taatccggca
    36901 gccttgagta acagcgaagg attggtggtg gcatcatggg gatgataatg gcgaatggac
    36961 tcaatatcgc cgctgtctgc cacgacagtg gtgaactgtt tgatgccgtc taactcgttc
    37021 ataggaaata ctccttgaaa agtaaagtgt tagatgagtg cgttaattca cacttctgag
    37081 aaatttcgct aaacgcatca aaaaagcata gcagacaggc atggtattgc tggattaagc
    37141 aggtaacatc agtgttatag gattattacc aaaacattat attaatggtg agagtttgga
    37201 cttgctcaaa gtctgtagac tccggcaggg taataatgtg cgccacgttg tggacagggg
    37261 ctgttgccca cacactttat ttgtgaacgt tacgtgaaag gaacaaccaa atggatgacc
    37321 agttaaaaca aagtgcactt gatttccatg aatttccagt tccagggaaa atccaggttt
    37381 ctccaaccaa gcctctggca acgcagcgcg atctggcgct ggcctactca ccaggcgttg
    37441 ccgcaccttg tcttgaaatc gaaaaagacc cgttaaaagc ctacaaatat accgcgcgcg
    37501 gtaacctggt agcggtgatc tctaacggta cggcggtgct ggggttaggc aacattggcg
    37561 cgctggcagg caaaccggtg atggaaggca agggcgttct gtttaagaaa ttcgccggga
    37621 ttgatgtatt tgacattgaa gttgatgaac tcgacccgga caaatttatt gaagtcgtcg
    37681 ctgcgctcga accgaccttc ggcggcatca acctcgaaga cattaaagcg ccagaatgtt
    37741 tctatattga acagaaactg cgcgagcgga tgaatattcc ggtcttccac gacgatcagc
    37801 acggcacggc aattatcagc actgccgcca tcctcaacgg cttgcgcgtg gtggagaaaa
    37861 acatctccga cgtgcggatg gtggtttctg gcgcgggtgc cgcagcaatc gcctgtatga
    37921 acctgctggt agcgctgggt ctgcaaaaac ataacatcgt ggtttgcgat tcaaaaggcg
    37981 ttatctatca gggccgtgag ccaaacatgg cggaaaccaa agccgcatac gcggtggtgg
    38041 atgacggtaa acgtaccctc gatgatgtga ttgaaggcgc ggatattttc ctcggctgtt
    38101 ccggcccgaa agtgctgacc caggaaatgg tgaagaaaat ggcccgtgcg ccaatgatcc
    38161 tggcgctggc gaacccggaa ccggaaattc tgccgccgct ggcgaaagaa gtgcgtccgg
    38221 atgccatcat ctgtaccggc cgttccgact acccgaacca ggtgaacaac gtcctttgct
    38281 tcccgtttat cttccgtggc gcgctggacg ttggcgcaac cgccatcaac gaagagatga
    38341 aactggcggc agtacgtgca attgcagaac tggcccatgc ggaacagagt gaagtggtgg
    38401 cttcagcgta tggcgatcag gatctgagct ttggtccgga atacatcatt ccaaaaccgt
    38461 ttgatccgcg cttgatcgtt aagatcgctc ctgcggtggc gaaagcggcg atggagtcgg
    38521 gcgtggcgac gcgtccgatt gctgatttcg acgtctacat cgacaagctg acggagttcg
    38581 tttacaaaac caacctgttt atgaagccga ttttctccca ggctcgcaaa gcgccgaagc
    38641 gcgtagttct gccggaaggg gaagaggcgc gcgttctgca cgcgactcag gaactggtga
    38701 ctctgggact ggcgaaaccg atccttattg gtcgtccgaa cgtgatcgaa atgcgcattc
    38761 agaaactggg cttgcagatc aaggcgggcg ttgattttga gatcgtcaat aacgaatccg
    38821 atccgcgctt taaagagtac tggaccgaat acttccagat catgaagcgt cgcggtgtca
    38881 ctcaggagca ggcgcagcgt gcgctgatca gtaacccgac agtgatcggc gcgatcatgg
    38941 ttcagcgtgg cgaagccgat gcaatgattt gcggtacggt gggcgattat catgaacact
    39001 ttagcgtggt gaaaaatgtc tttggttatc gcgatggcgt tcacaccgca ggtgcaatga
    39061 acgcgctgct gttgccgagt ggtaacacct ttattgccga tacctacgtc aatgatgagc
    39121 ctgatgcaga agagctggcg gagatcacct tgatggcggc agaaactgtt cgtcgttttg
    39181 gtattgagcc gcgtgtggct ttgttgtcgc actccaactt tggttcttct gactgcccgt
    39241 cgtcgagcaa aatgcgtcag gcgctggaac tggtcaggga acgtgcgcca gaactgatga
    39301 ttgatggtga aatgcacggc gatgcagcgc tggtggaagc gattcgcaac gaccgtatgc
    39361 cggacagccc tttgaaaggt tccgctaata ttctggtgat gccgaacatg gaagctgccc
    39421 gcattagtta caacttactg cgcgtttcca gctcggaagg tgtgactgtc ggtccggtgc
    39481 tgatgggcgt ggcgaaaccg gttcacgtgt taacgccgat tgcatcggta cgtcgtatcg
    39541 tcaacatggt ggcgctggcc gtggttgaag cgcaaaccca accgctgtaa ttctttttta
    39601 actctca
//
LOCUS       DAZGJT010000036        38518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZGJT010000036 DAZGJT010000000
VERSION     DAZGJT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.036"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1137)
                     /locus_tag="ACHKFK_002029"
     CDS             complement(<1..1137)
                     /locus_tag="ACHKFK_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001414312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9052374.1"
                     /translation="MALTDIQIKRAKPQDKPYTLNDGQGLSLLINPDGSKGWRFRFRF
                     AGKARLMSFGSYDLVSLAEAREKRDTARKQVANGIDPVEERKAQKIAQKLSIETSFEA
                     VSREWHAAKADRWTLAYREEIIKTFEQDVFPFIGKRPIAEIKSLELLEVLKRIEKRGA
                     LGKTRKVRQRCGEVFRYAIITGRAEYNPAPDLAIALAVPKQKHHPFLSAEELPYFVKD
                     LEAYTGSIITKNATKIVMLTGVRTQELRFATWEEVNLEKGIWEIPAERMKMRRPHLVP
                     LSSQVITLFNQLKPISSHYPYIFIGRNNRTKPISKESISQVIELLGYKGRATGHGFRH
                     TMSTILHEQGFDSAWIEMQLAHVDKNSIRGTYNHAQYLEKRLKML"
     gene            complement(1300..1394)
                     /locus_tag="ACHKFK_002030"
     tRNA            complement(1300..1394)
                     /locus_tag="ACHKFK_002030"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1358..1360),aa:Sec,seq:tca)
     gene            1687..3069
                     /gene="yicJ"
                     /locus_tag="ACHKFK_002031"
     CDS             1687..3069
                     /gene="yicJ"
                     /locus_tag="ACHKFK_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO9052375.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTIVNIPYCALGGVITNDPTQCISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     DDLRDIWQNDQWRIVGFLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSVTISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            3079..5397
                     /gene="yicI"
                     /locus_tag="ACHKFK_002032"
     CDS             3079..5397
                     /gene="yicI"
                     /locus_tag="ACHKFK_002032"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO9052376.1"
                     /translation="MKISDGNWLIQPDLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDSTLLALGNNDQRPDYAW
                     HEGTAFHLFNLQDGHEAVCEVPTADGSVIFTLKAARTGNTITVTGTGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            5692..7245
                     /locus_tag="ACHKFK_002033"
     CDS             5692..7245
                     /locus_tag="ACHKFK_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HFO9052377.1"
                     /translation="MQMITSRQNRLLRFLLPRREYTTIVTIAGYLNVSEKTIQRDLRL
                     LEQWLGQWRINVEKRAGAGVMLSAENIADLLHLDHLLVAECEEIDGVMNNARRVKIAS
                     QLLSETPNETSISKLSERYFISGASIVNDLRVIESWLAPLGLSLIRSPSGTHIEGSEG
                     QVRQAMALLINGIINHNEPQGVVYSRLDPGSYKALVHYFGEEEVLFVQSLLLDMENEL
                     SWSLGEPYYVNIFTHILIMMYRNTHGNALSREEDQTRQYDENIFNVASQMIHKIEQRI
                     AHTLPDDEVWFIYQYIISSGVAIDGQKDVSIISHMQASNEARLITWRLITVFSDIVDC
                     DFSEDSALYDGLLVHIKPLINRLNYRIHIRNPLLEDIKAELADVWRLTQYVVNQVFKT
                     WGENAVSEDEVGYLTVHFQAAMERQIARKRVLLVCSTGIGTSHLLKSRILRAFPEWTI
                     VDVISAANLSQVLPDNIELIISTINLPTVTMPVAYVTAFFNDADIKRVTEMVITEKLH
                     HATSRVVEI"
     gene            7272..7742
                     /locus_tag="ACHKFK_002034"
     CDS             7272..7742
                     /locus_tag="ACHKFK_002034"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007370547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose PTS transporter subunit IIA"
                     /protein_id="HFO9052378.1"
                     /translation="MDITKILNTNRVILDMQATNKAEAIEELTNLLKKDGAIDCRETF
                     IKDVWQREAEGSTGFENHIAIPHGKSSAVINTTLAIGRTRQDIPWETLDGSSVRCIIL
                     FAVRLEDQNTTHIRLLSQVAGALADDDIIEQLLVETSPQKIIDLFSQYAQSDVC"
     gene            7760..8074
                     /locus_tag="ACHKFK_002035"
     CDS             7760..8074
                     /locus_tag="ACHKFK_002035"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001028073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO9052379.1"
                     /translation="MNIVCVAACTAGIAHTYIAREKLIKGAKALGHTIKVETQGTIGT
                     ENELQAADTSAADVVILAVDVKIKGEERFTNKRIVRVKTEIVIKSPVQFLEKVEKSLG
                     NK"
     gene            8099..9190
                     /locus_tag="ACHKFK_002036"
     CDS             8099..9190
                     /locus_tag="ACHKFK_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO9052380.1"
                     /translation="MKENKIPVSQEIKKHLLTGISWMIPLIVAAGICIALGQVLGGTD
                     VGEKTGTIPWMLNQIGGWGMGLIVPLISAAIAYSIADRPGFAPGLIVGFLCGQIHTGF
                     IGGMLGGFLVGYTILLLKRYIRLPQSMQGLMPIMVLPVLSTIIGGLLMMTLIGKPIAW
                     LQEALIHLLESMQGGSRFLMGAILGAMATFDFGGPVNKTMSLFSDGLLVSGVYGPEAV
                     KFVGSIIPPFGITLSFLLTRHKYTRAEREALKAAFPMGICMITEGVIPIAARDLLRVV
                     GSCVVASAVAGGLIMVWGVESPVPHGGMFVVPLFTHPLLFCLALGIGTVICGVMLSLW
                     KKPVTERDEEFDELSDQKLKDEEITFTLE"
     gene            9202..10053
                     /locus_tag="ACHKFK_002037"
     CDS             9202..10053
                     /locus_tag="ACHKFK_002037"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9052381.1"
                     /translation="MFADMKSMVMKAWHEHYALLAINCMNLESAHAAIRVAEKNRAPI
                     ILNLYQGHLAHFPAPVAAAVVKTLAESASVPVTLALDHGKNPDCIRQAFRAGFSGLMI
                     DASAFPLEENVRQTRAVVELAASAQLCVEGELGHLADAPRYDQAANADLMTQPTDVEP
                     FIAQTGIDLLAVSVGTAHGMYAPGVVPAIDFQRLAEIFHCSSVPLALHGGSGTPFDQL
                     QLCTTLGVAKINVGAAIFERGKSALLHTLCHDISIELVDALKAMELAFEEAITPYLQA
                     SGSIGKA"
     gene            10134..10994
                     /locus_tag="ACHKFK_002038"
     CDS             10134..10994
                     /locus_tag="ACHKFK_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO9052382.1"
                     /translation="MLVSMKELLQPTRQHGFAIGAFNVADNCFLRAVVDEAEATNTPA
                     IIAIHPSEHDFVGDAFFAHVREITQRSPVPFVLHLDHGASVEHVLRAIRCGFTSVMID
                     GSLLPYEENVALTAEVVRLAHAVGVSVEGELGTIGQTGTSVEGGVSQVTYTDPAQAAD
                     FIARTGADTLAVAIGTAHGIYPKGMQPKLQMNILRDIAGRIDIPLVLHGGSANPDAEI
                     AESVTLGVGKINISSDMKYAYFQKVREILAKESWWNPNVIYPDAINAAREVIRHKMKL
                     FGSFGKASLY"
     gene            11042..11974
                     /locus_tag="ACHKFK_002039"
     CDS             11042..11974
                     /locus_tag="ACHKFK_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO9052383.1"
                     /translation="MHYLGLDIGGTKIAAVVMDAHGWEIRRYRCPTQKSTYQQFVSCV
                     VALIEQIRRDVQRPMLTGIALPGSISPLTGLIKNANIQVINGHALQADLQQLLGQPVV
                     IANDGNCFALSEACDGAGQDYDVVFGITLGTGCGGGIAIKQRPFIGAWGNAAECGHIT
                     LPGYTEQEDGPSVSCYCGKHNCVESFVSGSGFSERYQQMTGNLLTPAAIVTLAQRGDA
                     CAMQQVARFRQQLARTLATIVNVVDPGVIVIGGGLSNVELLITDLNAEVAPLVFTDQF
                     TTPIVKAQHGDSSGMRGAAWLAMRNGEANETFTN"
     gene            11955..12635
                     /locus_tag="ACHKFK_002040"
     CDS             11955..12635
                     /locus_tag="ACHKFK_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HFO9052384.1"
                     /translation="MKRSQINYVIDKAHAIAQTFRVCLPEFAYFTVDDWLQRERDNWQ
                     EVVDLQLGWDITDFGRGDFNQTGLTLLTMRNGALGSAAYPKPYAEKMLQIQQDQQTPW
                     HFHTHKMEDILNRGGGDLCMQLAWANEANLCDDQRVITVSVDGQRRTMKPGETLVLKP
                     GQGICLPPRLYHRFWAEKAFVLGWEISMVNDDQHDNYFLEPGGRFPAIEEDEPVKWLL
                     CSEYGILR"
     gene            complement(12679..14388)
                     /gene="yicH"
                     /locus_tag="ACHKFK_002041"
     CDS             complement(12679..14388)
                     /gene="yicH"
                     /locus_tag="ACHKFK_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9052385.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWQRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(14509..15900)
                     /gene="xanP"
                     /locus_tag="ACHKFK_002042"
     CDS             complement(14509..15900)
                     /gene="xanP"
                     /locus_tag="ACHKFK_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO9052386.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16180..17385
                     /gene="gltS"
                     /locus_tag="ACHKFK_002043"
     CDS             16180..17385
                     /gene="gltS"
                     /locus_tag="ACHKFK_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO9052387.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLILKKSMGWEVNFDMSLRDPLMLAFFATIGLNANLASLRAGGRVVGVFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFTG"
     gene            complement(17419..19500)
                     /gene="recG"
                     /locus_tag="ACHKFK_002044"
     CDS             complement(17419..19500)
                     /gene="recG"
                     /locus_tag="ACHKFK_002044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO9052388.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFNAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSELETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSTNDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAVAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRIDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMTSFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(19506..20195)
                     /gene="trmH"
                     /locus_tag="ACHKFK_002045"
     CDS             complement(19506..20195)
                     /gene="trmH"
                     /locus_tag="ACHKFK_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO9052389.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWSTMQAAG"
     gene            complement(20202..22310)
                     /gene="spoT"
                     /locus_tag="ACHKFK_002046"
     CDS             complement(20202..22310)
                     /gene="spoT"
                     /locus_tag="ACHKFK_002046"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO9052390.1"
                     /translation="MYLFESLNQLIQNYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(22329..22604)
                     /gene="rpoZ"
                     /locus_tag="ACHKFK_002047"
     CDS             complement(22329..22604)
                     /gene="rpoZ"
                     /locus_tag="ACHKFK_002047"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO9052391.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(22659..23282)
                     /gene="gmk"
                     /locus_tag="ACHKFK_002048"
     CDS             complement(22659..23282)
                     /gene="gmk"
                     /locus_tag="ACHKFK_002048"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO9052392.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            23540..25222
                     /gene="ligB"
                     /locus_tag="ACHKFK_002049"
     CDS             23540..25222
                     /gene="ligB"
                     /locus_tag="ACHKFK_002049"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO9052393.1"
                     /translation="MKVWMAILISILCWQSSAWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGNETRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVRL
                     ISAVPQTVSGPLANSTLQGEIFLQRKGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPHLMTDRLKDLATAGFTLTQTYTRAVKNADEVAHVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLV
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARQQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(25219..25836)
                     /gene="yicG"
                     /locus_tag="ACHKFK_002050"
     CDS             complement(25219..25836)
                     /gene="yicG"
                     /locus_tag="ACHKFK_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO9052394.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(26088..26951)
                     /gene="yicC"
                     /locus_tag="ACHKFK_002051"
     CDS             complement(26088..26951)
                     /gene="yicC"
                     /locus_tag="ACHKFK_002051"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO9052395.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIIARETEGQALKVLIEQRL
                     EGVTAEVAKVRAHMPEILQWQRERLIAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            27078..27794
                     /gene="rph"
                     /locus_tag="ACHKFK_002052"
     CDS             27078..27794
                     /gene="rph"
                     /locus_tag="ACHKFK_002052"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO9052396.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            27860..28501
                     /gene="pyrE"
                     /locus_tag="ACHKFK_002053"
     CDS             27860..28501
                     /gene="pyrE"
                     /locus_tag="ACHKFK_002053"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO9052397.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(28538..29134)
                     /gene="slmA"
                     /locus_tag="ACHKFK_002054"
     CDS             complement(28538..29134)
                     /gene="slmA"
                     /locus_tag="ACHKFK_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO9052398.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(29241..29699)
                     /gene="dut"
                     /locus_tag="ACHKFK_002055"
     CDS             complement(29241..29699)
                     /gene="dut"
                     /locus_tag="ACHKFK_002055"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO9052399.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(29677..30897)
                     /gene="coaBC"
                     /locus_tag="ACHKFK_002056"
     CDS             complement(29677..30897)
                     /gene="coaBC"
                     /locus_tag="ACHKFK_002056"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO9052400.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVNRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            31069..31737
                     /gene="radC"
                     /locus_tag="ACHKFK_002057"
     CDS             31069..31737
                     /gene="radC"
                     /locus_tag="ACHKFK_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9052401.1"
                     /translation="MKNNAQLLMPREKMLKFGISVLTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            31954..32190
                     /gene="rpmB"
                     /locus_tag="ACHKFK_002058"
     CDS             31954..32190
                     /gene="rpmB"
                     /locus_tag="ACHKFK_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO9052402.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            32211..32378
                     /gene="rpmG"
                     /locus_tag="ACHKFK_002059"
     CDS             32211..32378
                     /gene="rpmG"
                     /locus_tag="ACHKFK_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO9052403.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            32476..33285
                     /gene="mutM"
                     /locus_tag="ACHKFK_002060"
     CDS             32476..33285
                     /gene="mutM"
                     /locus_tag="ACHKFK_002060"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO9052404.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(33324..33803)
                     /gene="coaD"
                     /locus_tag="ACHKFK_002061"
     CDS             complement(33324..33803)
                     /gene="coaD"
                     /locus_tag="ACHKFK_002061"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO9052405.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVELAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(33811..35088)
                     /gene="waaA"
                     /locus_tag="ACHKFK_002062"
     CDS             complement(33811..35088)
                     /gene="waaA"
                     /locus_tag="ACHKFK_002062"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO9052406.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            35501..36559
                     /gene="rfaQ"
                     /locus_tag="ACHKFK_002063"
     CDS             35501..36559
                     /gene="rfaQ"
                     /locus_tag="ACHKFK_002063"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO9052407.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRNSFTHLVPLQGGNVVESNLSVLTPLGLESLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            36556..37680
                     /gene="waaG"
                     /locus_tag="ACHKFK_002064"
     CDS             36556..37680
                     /gene="waaG"
                     /locus_tag="ACHKFK_002064"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO9052408.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDRVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            37673..38470
                     /gene="rfaP"
                     /locus_tag="ACHKFK_002065"
     CDS             37673..38470
                     /gene="rfaP"
                     /locus_tag="ACHKFK_002065"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO9052409.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEVLKISVIDLHRAQIRAKVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDIIKQEQGLLSQAEEKATKIRERTIRKSL"
BASE COUNT         9254 a         9343 c        10223 g         9698 t
ORIGIN      
        1 taacatcttt aatcttttct ctaaatattg agcatgatta tatgttcccc taatgctatt
       61 tttatcaaca tgagctagtt gcatttcaat ccatgcacta tcaaaacctt gttcatgtag
      121 tattgtcgac attgtatgtc taaacccgtg gcctgttgca cgtcccttat aaccaagtaa
      181 ttcgatcact tgtgatatac tttctttaga aattggttta gtacgattat tacgtccaat
      241 aaatatataa ggataatggc tagagatggg ttttaattga ttaaataggg taatcacttg
      301 agatgataaa ggtacaaggt gaggccgacg cattttcatg cgttctgctg gtatttccca
      361 tattcctttt tctaaattga cttcttccca tgtggcaaaa cgcagctctt gtgttcttac
      421 tccggtaagc atcacaatct tggttgcatt tttagtgatt atgctgccag tgtaagcctc
      481 cagatctttg acaaaataag ggagttcttc tgctgataga aaaggatgat gtttttgctt
      541 cggtacagct aatgcgatag ctaaatcagg tgctggattg tattcagcac ggccagtgat
      601 gatcgcgtag cgaaacacct ctccgcatcg ttgacgtact tttctggttt tccccaacgc
      661 tccacgtttt tctatgcgtt taagtacttc aagcagttct agggatttaa tttcagctat
      721 tggccgttta ccaatgaaag gaaaaacatc ctgttcaaat gttttaataa tttcttcgcg
      781 ataagctaga gtccaacgat ctgcttttgc tgcgtgccat tctctgctaa ctgcctcaaa
      841 agaggtttca attgataatt tctgagcaat tttctgagcc ttacgttctt ctactggatc
      901 aatgccgttt gcaacctgtt tacgtgccgt atcgcgtttc tcacgtgctt ctgcgaggct
      961 tactaaatca tagctgccaa atgacattaa ccgcgctttc ccagcaaatc tgaaacggaa
     1021 acgccagcct ttcgagccat ctggattgat aagcaacgac agaccttgtc catcgttcaa
     1081 tgtgtatggc ttgtcttgtg gttttgcacg tttgatctgt atatcggtta gagccatgtg
     1141 tataactgaa aactacgtat aaaaaatcta tacacattac tatacatatt tttcgcggat
     1201 tcagggagat gaactcggac tattacggat aagatgatta ttcatcgtat tgaatacaaa
     1261 gggttttatg gagcgtctcg gaactggctg gatgaagttt ggcggaagat cacaggagtc
     1321 gaacctgccc gggaccgctg gcggccccaa ctggatttga agtccagccg cctcaccgga
     1381 gacgacgatc ttccgcgcct cgattgctac atggaggcgg ggcgcattat agctacttcc
     1441 ttgagtttct acatccccca gatcagtttt ttccgcctaa tacacgcagc gtattatcac
     1501 gaaagcatta tagttcatat aaaactcata ttttacaatg atatatgagc aaaattaacg
     1561 ctaccacgat cacataaaac aagaatcata aattaataac cagatatcgg aatattcgct
     1621 ctcgcaggga tggtttacaa aatgcgttat cggtctcagc acccctatag catcaaggaa
     1681 aagcagatga agagtgaagt gttgtccgtt aaagagaaaa ttggttatgg catgggagac
     1741 gccgccagcc acattatttt cgataacgta atgttatata tgatgttctt ttataccgat
     1801 atttttggca ttcctgccgg atttgtcgga accatgtttt tggtcgctcg tgcactggat
     1861 gcgatttccg atccttgcat ggggttgttg gccgatcgaa cgcgctctcg ctggggtaaa
     1921 tttcggccgt gggtgctgtt tggcgcactg ccattcggga tcgtctgtgt actggcctat
     1981 agcacgccag atctcagtat gaacggaaaa atgatttatg cagcaattac ttacacccta
     2041 cttaccttac tttataccat cgtcaatatc ccttactgcg cattgggtgg tgtaatcacc
     2101 aatgacccga ctcagtgtat ctcgctgcaa tcctggcgtt ttgtgctggc gaccgcggga
     2161 ggcatgctct ctactgttct gatgatgcca ctggttaatt taattggtgg tgataataaa
     2221 ccactcggtt tccagggcgg tatcgcggtc ctttccgtgg tggcatttat gatgctggca
     2281 ttctgtttct tcaccactaa agaacgcgtt gaagcaccac ctacaacgac gtctatgcgg
     2341 gatgatttac gtgatatctg gcaaaacgac cagtggcgta ttgtcggttt cctgacaatt
     2401 ttcaatatcc tggcggtgtg cgtacgcggt ggggcgatga tgtattacgt cacatggatt
     2461 ttgggtacgc cggaagtgtt tgtcgctttt ctcaccactt attgcgtggg taacctgatt
     2521 ggctccgcac tggcaaaacc gctgaccgac tggaaatgta aagtcactat cttctggtgg
     2581 acgaacgccc tgctggcagt gattagcctc gcgatgttct ttgttcctat gcaggccagc
     2641 atcaccatgt ttgtcttcat cttcgtgatt ggtgtgttgc atcaactggt gacacctatc
     2701 cagtgggtaa tgatgtccga taccgtcgac tacggcgagt ggtgtaatgg taaacgcctg
     2761 accgggatca gttttgctgg cacgctgttt gtgctcaaac tggggttggc cttcggtggt
     2821 gcccttatcg gctggatgct ggcttatggc ggatatgatg cggcagaaaa agcgcagaac
     2881 agcgtcacaa ttagcatcat tatcgcgcta ttcacgattg ttccggcgat ctgttacttg
     2941 ctgagcgcga ttatcgctaa acgctactac tcgctcacga cgcacaatct gaaaaccgtt
     3001 atggaacagc tggcgcaggg caaacgccgt tgccagcaac aattcacctc tcaagaagtg
     3061 cagaactaag gaacggcaat gaaaattagc gacggaaact ggttgattca acctgacctc
     3121 aatttgattc acccgcttca ggtgttcgag gttgaacagc agggtaatga aatggtggtc
     3181 tatgctgccc ctcgtgatgt gcgtgaacgt acctggcagc ttgatactcc tttatttacg
     3241 ctgcgctttt tctccccaca ggaaggtatt gtcggtgtac ggattgagca ttttcagggg
     3301 gcgctgaata acggtcctca ttacccgctc aatattttgc aggacgtgaa ggtcactatc
     3361 gaaaacacag aacgttacgc tgagtttaaa agtggcaact taagtgcgcg tgtcagcaaa
     3421 ggtgagttct ggtcactgga ttttctgcgc aacggcgaac gtattaccgg tagtcaggtg
     3481 aaaaataacg gctacgtgca ggacacgaat aatcaacgaa attatatgtt tgagcggctg
     3541 gatcttggcg ttggcgaaac agtttacggt ctgggagagc gctttactgc cctggtgcgc
     3601 aatggtcaga cggtagagac ctggaaccgg gacggcggca caagtactga acaggcgtat
     3661 aaaaatatcc cgttttatat gactaaccgt ggttatgggg tactggtcaa tcatcctcaa
     3721 tgcgtctctt ttgaagtggg atcggagaaa gtctccaaag tacagttcag cgttgagagt
     3781 gaatatctcg aatactttgt tatcgacggc ccgacgccga aagcggtact tgatcgttat
     3841 acccgtttta ctggccgtcc ggcgctgccg cccgcgtggt ccttcggtct gtggctaacc
     3901 acttcattta ccaccaacta cgacgaagcg acggtaaaca gctttatcga tggtatggcg
     3961 gaacgcaatc tgccgctgca tgttttccac tttgactgtt tctggatgaa agccttccag
     4021 tggtgcgatt ttgagtggga cccgctgact tttccggacc cggaagggat gatccgccgt
     4081 ctgaaagcga aagggctgaa aatctgcgtc tggattaacc cctatattgg gcaaaaatcc
     4141 cccgtcttta aagagttaca agagaaaggt tatttactca aacgcccgga cggttcgctg
     4201 tggcagtggg ataaatggca gccaggtctg gcgatttatg actttaccaa tccggatgcc
     4261 tgcaaatggt acgccgacaa actgaaaggt ctggtcgcga tgggcgttga ttgctttaag
     4321 accgactttg gcgaacgtat tccaaccgat gttcagtggt ttgatggttc cgatccgcag
     4381 aaaatgcata accattatgc gtacatctac aacgaactgg tgtggaacgt gctcaaggac
     4441 accgttggtg aggaagaagc cgtcttgttt gcccgctcgg cctccgttgg tgcgcagaaa
     4501 tttccggtac actggggtgg cgactgttac gctaactacg aatcaatggc ggaaagcctg
     4561 cgcggtggtt tgtctattgg cctttcaggt tttggcttct ggagccacga tatcggcggc
     4621 tttgaaaata ccgctccggc gcacgtttac aaacgctggt gcgcgtttgg tttgctttcc
     4681 agccatagcc gtttacatgg cagcaaatct tatcgtgtgc cgtgggccta cgatgatgag
     4741 tcctgtgatg tggtgcgctt cttcacgcaa ctgaaatgcc gcatgatgcc gtatctgtat
     4801 cgtgaagctg cacgtgcaaa cgcccggggt acgccgatga tgcgggccat gatgatggag
     4861 ttcccggacg atccggcttg tgattacctt gaccgtcaat atatgttagg cgacaacgtg
     4921 atggttgcgc ctgtgttcac tgaagcgggc gatgtgcagt tctacctgcc ggaaggtcgc
     4981 tggacacacc tgtggcacaa cgatgaactc gacggtagtc gctggcataa acagcagcac
     5041 agcttcctga gtctgcccgt ttatgtgcgt gatagcaccc tactggcgct gggcaacaac
     5101 gatcaacgtc ccgattacgc gtggcacgaa ggtacggcat tccacctctt taatctgcaa
     5161 gacgggcatg aggctgtctg tgaagtgccc actgctgacg gttcggtgat ctttacttta
     5221 aaagcagcac gtactggcaa cacgattact gtgactggta cgggcgaggc gaagaactgg
     5281 acactgtgct tgcgcaatgt tgtgaaagta aatggtctgc aaggcggttc gcaggctgaa
     5341 agtgagcagg gactggtggt gactcctcaa gggaatgcgc tgacaattac gttgtaatct
     5401 attgaagaat gccggatacg aatggttttg tgtcgtatcc ggtaaataat ctcacatctg
     5461 gtaataaata tttcttccat cgtatttgcg agcatgatca cgattattca tcacgaaaat
     5521 gtgatctgtg ctgaaaaata tcccttaacc gccttgcttt gttttatcac tgtccggatt
     5581 acggacagat tttagtattg ttgtgaaatt attctgcttt atatttaccc tcagagatgg
     5641 caactgaagc tcttccatat ggggcttctt aatctgtgat aagggtaaaa aatgcaaatg
     5701 attacctcgc gacaaaacag attattacga ttccttttac ctcgaaggga atatacgact
     5761 attgttacga ttgccggcta tttaaatgtt tcggaaaaaa ccattcaacg tgatttacgt
     5821 ttacttgagc aatggctggg acaatggaga ataaatgttg agaagcgtgc tggcgcgggt
     5881 gtgatgttaa gtgcggagaa tattgctgat ttgctgcatc ttgatcattt gttggtggca
     5941 gaatgtgaag agattgatgg tgtaatgaat aatgccaggc gcgttaaaat agcgtcgcag
     6001 ttattaagtg aaacaccgaa tgaaacgtcg atcagtaaat tgtcagaacg ctactttatc
     6061 agcggagcct ctattgttaa tgacctgaga gtaattgagt cctggcttgc gccgttgggg
     6121 ttatcgttga tccgcagtcc aagtggtacg catattgaag gtagtgaagg gcaagtccga
     6181 caggcaatgg cattactgat taacggcatt attaaccata atgagccgca aggtgtcgtg
     6241 tattcacgtc tggatcccgg aagctataaa gcattagtcc attattttgg agaagaagag
     6301 gtgttatttg tccagtcatt gttactggat atggaaaatg aattaagttg gtctttggga
     6361 gaaccttatt acgttaacat ttttactcac atccttatta tgatgtatcg aaacacgcac
     6421 gggaatgcgt tatcaagaga agaagatcaa accaggcaat atgatgaaaa tatctttaat
     6481 gttgccagtc agatgattca taagatagaa caacgaattg cacatacatt gcccgatgat
     6541 gaagtctggt ttatttatca atatatcatt tcatcaggtg tggcgattga tggacaaaaa
     6601 gatgttagca ttatttcaca tatgcaggcc agcaatgaag cgcgtctgat tacctggcgt
     6661 ttaattacgg tattcagtga catcgtggac tgcgatttta gtgaagacag cgcattatat
     6721 gatggcttat tggtgcatat taagccgctg attaaccgtc taaattatcg cattcatatc
     6781 cgtaatccat tgctggaaga tattaaagca gaattagcgg atgtctggcg gttgacgcaa
     6841 tatgtggtga atcaggtatt taaaacctgg ggtgagaatg cagtgagcga ggatgaagtg
     6901 ggttacctga ccgttcattt tcaggctgcg atggagcggc aaattgcccg taaacgtgta
     6961 ttactggtct gttcaaccgg aatcggaact tcgcatctac tgaaaagccg tattctgcga
     7021 gcatttcctg aatggacgat tgttgatgtt atttcagcag cgaatttatc acaggttttg
     7081 cctgacaata tcgaactgat tatttcgacc attaatttgc ctacagtcac tatgccggtc
     7141 gcttatgtta ccgctttttt taatgatgcc gatattaagc gggtcactga aatggtgatt
     7201 acggaaaaat tacatcatgc gacgtctcgg gtcgttgaaa tttaatgtca gaattttatg
     7261 aggtatgacg aatggacata acaaaaatcc tgaatacgaa tcgcgttatt ttagatatgc
     7321 aagcaacgaa taaagcggag gcgattgaag aattaactaa tcttctcaaa aaagatggtg
     7381 cgatcgattg tcgggaaacc tttattaaag atgtctggca gcgcgaagcg gaaggctcga
     7441 ctggttttga aaatcatatt gcgatcccgc atggtaaatc ttctgcggta attaatacta
     7501 cgctggcaat tggccgtacc cgccaggata ttccctggga aacgctggat ggcagtagcg
     7561 tgcgctgtat tattttattt gcggtacgtc ttgaagatca aaataccacc catatccgtt
     7621 tactttccca ggtcgcgggc gcgctggcgg atgatgacat catagaacaa ttgttagttg
     7681 agacgagtcc gcaaaaaatc attgatctct ttagtcagta tgcacaatcc gatgtctgct
     7741 aaataacagg aagtatgtca tgaatattgt ctgtgttgcc gcctgtacgg cgggtattgc
     7801 tcatacctat attgctcgtg aaaagttaat caaaggagca aaagctctgg gccataccat
     7861 taaagttgaa acccagggaa ccattggtac agaaaatgaa ttacaggcgg cagatacctc
     7921 tgctgctgac gtcgtgattc ttgctgtaga tgtgaaaata aaaggagaag aacgcttcac
     7981 caataaacgt attgtgcgag taaagacaga gatcgtcatt aaatcgcccg ttcaatttct
     8041 tgaaaaggtc gaaaaatcac tgggaaacaa atgatcctta tactggaggg atcttaatat
     8101 gaaagaaaat aaaattccgg tttcacagga aattaaaaaa catttattaa ccggcatctc
     8161 gtggatgata ccccttattg tggccgccgg gatatgtatt gcgttggggc aagttcttgg
     8221 cggtacagat gttggtgaaa aaacaggaac aatcccgtgg atgcttaatc agattggcgg
     8281 ctggggaatg gggctgattg tgccgctgat tagcgcggca attgcctatt ctattgccga
     8341 tcgtcccgga tttgccccgg gcctgattgt cggctttctc tgcgggcaaa tccataccgg
     8401 atttattggc ggtatgttgg gtggtttcct ggtgggctac accattttgc tacttaaacg
     8461 ctatatccgc ctgccgcagt cgatgcaagg actcatgcca atcatggtgt tgccggtatt
     8521 aagcaccatt atcggtgggc tgttaatgat gacgctgatt ggtaaaccca tcgcctggtt
     8581 acaagaagca ctgattcatt tgctcgagtc gatgcagggc ggatcgcgct tcctgatggg
     8641 ggccatactt ggcgcgatgg caaccttcga tttcggtggt ccggtcaaca aaaccatgtc
     8701 gctgttttcc gatggcttgc tggtcagtgg cgtctacgga ccagaagccg tgaaatttgt
     8761 cggctcaatc atccctccgt ttggcatcac actttcgttc ttactgacac gccataaata
     8821 cacccgtgcg gagcgcgagg cgctgaaagc cgcgtttccg atggggatct gcatgatcac
     8881 tgaaggagtc attccgatcg ccgcgcgtga cctgctgcga gtagtgggat cttgtgtggt
     8941 ggcatcggcg gtcgcgggcg gcttaatcat ggtgtggggc gtggagagtc cggttccgca
     9001 tggcggcatg tttgttgtgc ctctgttcac ccatcctctg ctgttttgcc tggcgctggg
     9061 tataggcacg gtgatttgtg gcgtgatgct ttcactctgg aagaaaccgg taacggaacg
     9121 cgacgaagag tttgacgaac tgagcgatca aaagctgaaa gatgaagaga tcactttcac
     9181 cctcgaataa ccggaggccc tatgtttgcc gatatgaaaa gtatggtgat gaaagcctgg
     9241 catgaacatt acgccttgct ggcgatcaac tgtatgaatc tggaaagtgc tcacgcagct
     9301 atccgcgtgg cggagaagaa tcgcgctccg atcattctca atctctatca ggggcatctg
     9361 gcgcattttc ctgcacccgt cgcggcggca gtggttaaaa cgctcgcgga atctgcctca
     9421 gtaccggtca ctctggcgct tgatcatggc aaaaatccgg actgtattcg tcaggcgttt
     9481 cgtgctggtt tcagcggatt aatgattgat gcttctgctt ttcccctgga ggaaaacgtg
     9541 cgccagacca gggcggtggt ggaactcgcc gccagcgcgc agctctgcgt ggaaggcgaa
     9601 ctgggccatc tggcagatgc gccgcgttac gaccaggccg ccaatgcaga cctgatgacg
     9661 caacccactg atgtggaacc gtttatcgcg cagacaggca ttgatctact ggccgtctcg
     9721 gtaggcacgg cgcacggcat gtacgcgcct ggtgttgtac ctgctattga ttttcagcgt
     9781 ctggcggaga ttttccactg ctcgagcgtg cctctggcat tacatggcgg tagcggtacg
     9841 ccgttcgatc agctccagct ctgtacgacg ctgggggtag caaaaatcaa cgtgggggcg
     9901 gccatttttg aacgggggaa atccgcgctc ctgcataccc tctgtcacga tatatccatt
     9961 gaactggtgg acgcgctgaa agctatggaa ctggccttcg aagaagcgat tacaccgtat
    10021 ttacaggcga gcggctctat cggcaaagcc tgattcattt tctgtcatgg cctttatgcc
    10081 agtgcaataa cgcaccggca gggcggacta cacgctgaaa ctggagattt aacatgttag
    10141 tttcgatgaa agaattactg caacctaccc gccagcacgg ctttgccatt ggcgcattta
    10201 atgtggcgga taactgtttc cttcgcgcag tcgtggatga agcagaagcg acaaatactc
    10261 cggcgattat tgccatccat cccagcgaac atgattttgt gggcgatgcc tttttcgctc
    10321 acgttcgcga aattacccag cgtagtccgg tcccgtttgt actgcacctc gatcatggtg
    10381 cctctgtaga gcatgttttg cgtgcgattc gctgcggatt tacctcagta atgatcgacg
    10441 gttcgctgct gccgtatgaa gaaaacgtgg cgctgaccgc agaagtggtt cgcctcgctc
    10501 acgcggttgg cgtgtcggtt gagggtgaac tgggaaccat cggacaaaca ggaacgtcgg
    10561 tagaaggtgg tgtttcacaa gtgacctaca cagatccggc gcaggccgcc gattttattg
    10621 ctcgtacagg tgccgacaca ctggctgtgg cgattggtac agcgcatggc atctatccaa
    10681 aagggatgca gccgaagctg caaatgaaca ttttgcgcga cattgccgga cgtattgata
    10741 ttccgctggt gctacacggt ggttcggcaa acccggacgc ggagattgct gaatcagtta
    10801 cgctcggcgt cggcaaaatc aatatctcca gcgatatgaa atacgcctat ttccagaaag
    10861 tgcgtgaaat tctcgctaaa gagagctggt ggaacccgaa cgtcatctat ccggatgcga
    10921 ttaacgcggc gcgggaagtc attcgccaca aaatgaaact gttcggttcg ttcggtaaag
    10981 catcactgta ctaagccaga agggcggggt gacccgcctt ttacgcaaca aggaacgaga
    11041 tatgcactat ctggggttgg atattggtgg gaccaaaatc gccgccgttg tcatggatgc
    11101 gcatggctgg gagattcgcc gttaccgctg cccgacgcaa aagtcgacat atcaacaatt
    11161 tgtctcatgc gttgtggcgc ttatcgagca gattaggcgg gacgttcaac gaccgatgct
    11221 gacagggatc gccttacccg gcagtatctc gccactcact ggcctgatta aaaacgcgaa
    11281 tattcaggtg attaacggtc atgcgttaca ggctgatttg cagcaattgc ttgggcaacc
    11341 ggtggtgata gccaatgatg gtaactgttt cgcgctatca gaagcttgcg acggtgccgg
    11401 gcaagattat gacgtggtat ttggtattac gcttggtacg ggctgcggcg gtggcattgc
    11461 catcaagcaa cgaccgttta taggggcctg gggaaatgct gccgaatgcg gtcatatcac
    11521 gttgccaggc tatacggagc aggaagatgg tccatcagtc agttgctatt gcggcaaaca
    11581 caactgcgtg gagtcgtttg tttccggcag cggttttagt gaacgctatc aacagatgac
    11641 tggtaacttg ctcactcctg cggcgattgt caccctggca caacgtggtg atgcttgtgc
    11701 catgcagcag gtggcacgtt ttcgccaaca gcttgcccgc acgctggcaa ccatcgttaa
    11761 tgttgttgac cctggcgtga ttgtgatcgg cggcgggctt tcgaatgtgg aactgcttat
    11821 caccgatctg aacgcagaag tcgctccact ggttttcacc gatcaattca ccactcccat
    11881 tgtaaaagca cagcacggcg acagtagcgg aatgcgtggc gctgcctggc ttgctatgcg
    11941 caacggagaa gccaatgaaa cgttcacaaa ttaattatgt tattgataaa gcacatgcca
    12001 ttgcgcagac cttccgcgtt tgtttgccag agttcgccta ttttacggtg gatgactggc
    12061 tgcagcggga acgggataac tggcaagaag tggtcgactt acagctcggt tgggatatca
    12121 ccgattttgg tcgtggtgat ttcaaccaaa cgggactaac actgctaacg atgcgcaacg
    12181 gcgcgctggg gtcggcggca tatccaaaac cgtatgctga gaaaatgttg cagatccaac
    12241 aagatcagca gactccctgg cattttcaca cccacaaaat ggaagatatc ctcaatcggg
    12301 gcggtggaga cttatgcatg cagttggcct gggccaacga agccaatctc tgtgatgatc
    12361 aacgagtcat taccgtgagt gttgatggtc agcggcgtac catgaaacct ggtgaaacgc
    12421 tggtgctaaa gccggggcag ggcatttgcc tgccgccgcg tctgtatcat cgcttctggg
    12481 cggaaaaagc gtttgttctc ggctgggaaa tctcgatggt aaatgatgac caacacgata
    12541 attattttct tgagccggga ggacgttttc cggcgataga agaagatgaa ccggtgaaat
    12601 ggttattgtg cagtgagtat ggaattttgc gctaataacg gggaacgcta gcgctgatat
    12661 ccgctagcgt ccttattttt actgcaccgg ctccgtcgac gtaacttcac cactggaaac
    12721 tacgcccgca ttcatggttt gagtcacttg ctgctttgcg gcattcacgg catgcaattg
    12781 cccgctaacc gtaggtttca gcgggacatt ggcctgaata tcgccactgg cggtaagctg
    12841 aatattgccg tcgccagtca acggtaattc aggccatccc caatgttgca aaatattcac
    12901 cggcacaccg cgtccattca ggctgatatg ggtctgacgt tgtggcgttt gtgaaacact
    12961 ggcggtggct tccagaatgc ctttttctgt aaatgcactc agttcgctga tattcaccgt
    13021 gctgctgttg gcagttagcg ccagcgacgg acgacggaca tcaacacgat taaacgttgc
    13081 ggcggcggca ttcagattcg ccgagccact ccagacgccc catttatgat cggtaaccag
    13141 cgtcagattg gcaccgtaac catcgagcgc ggtgagctgc cacgggaagt cagggtcgat
    13201 atcaatgatc agattgcggc tggcgctaaa tctcttcagt tgcaggctgt ttaaccaacc
    13261 gggtgtcgtt tccatccaca actgttgcca gttttttggc aaggtatatt ccagtccggc
    13321 aattgccgca tcatcgagga tcagcgtttt cccgtcgcgt tgccagtttc ctgacgttct
    13381 gaccataccc ccttcccagc ggctggtgaa ctggcgcagt gctacgccct gcggggaaaa
    13441 ttctgcgttt ataatcggat caaataaatg cagcgagcca taaataaact cgctggcgtt
    13501 catcgacagt ttgccttcct gtgtctgcca gtcatcttta ctgaaggtca tgttgcgcaa
    13561 gctgagatcg aggtcggtca ccgcccagtc cggaccttgc aaacgagcat cgatgacttc
    13621 caggcgacca atctgcaacg acgggacaga gcgtaatggc gcaaagaaat cggttagcga
    13681 tttttcgctt tgtagacgga tatccgccat gcgcaggttt tccacttgcc agctgccgtc
    13741 agcgttacgc tgcgcgtttc cggttaatgt cccgcgggcg atgtcggcac ccaggttagt
    13801 cagcgtaacg cgatcgttat caatactgcc ttcaatcagt acattggtgg caggaacatc
    13861 attgagcgaa agcgatccgg cactaaactg aatctgcgcc ttcgtaccca gcactttacc
    13921 ggcttctggt gaccacggaa ccacgccgcc atttacccgc tgcgcgctca gtttccattc
    13981 gctattcggg ctattaaacg ccatatcacg cagttgcaga cgatcggctt tgaacggtag
    14041 cggcgcggtc tggtcggtga gattcagcgt cccgttttcc agcaggatgg tatcgacatg
    14101 gcgtggttcg gtcagttgcc gactgcttag cgcaatgtcg acactttttg ccaccagggt
    14161 cgcgggctgg ccatcacggc caaacgtgac gttctccagc acgatatgag atggcgcgga
    14221 aaaacggtga tccatcgccc cgaaggccag atgatagtcg ctattctcgg aaacccatgc
    14281 gctgatatgt tctgctcccc agcgggtttg cagaagaaaa tagaggccag cgatcaccac
    14341 taacagagcg atgagaatgt agagaagcag cttcccaata aatttcatgg tcttccatcc
    14401 cgtgaaatgc acataaggga gttatgcacg atttacgcgc aatcctcaag gcgggaatgg
    14461 tgaaagaggt gagactgcgg cggtaattat cttaccgccg ccagtgaatt actgtttttc
    14521 tggggggaaa atcagattca gcacgatggc agtaataccg cccgcggcaa tcccggagga
    14581 gagcaggttt ttcagccatt caggggcaaa ctgcaaaatc agcggctgct gagacacgcc
    14641 cagaccgacc gccagcgaca gcgcgataat cagaatcgcc cggcggttca gcggctcacg
    14701 agaaacgata cgcacaccgg aggcggcgat ggtgccaaac attacaagcg ttgcgccgcc
    14761 cagaaccggt tctggaatgt gttgtacaaa accgctcact gccgggaaca gacccagcac
    14821 gatcaacatc agcgcgacga caaaaccgac atagcggctg gcaacaccgg ttaactggat
    14881 caccccgttg ttctgcccga agcaggagtt cgggaaggta ttaaataccg ccgagacaaa
    14941 cgagttcagg ccgtttgcca gcacgccgcc tttcaggcgt ttcatgtaca gcggaccgga
    15001 aaccggctgt tcggaaacgt cagaggtcgc cgtgatatcg ccaatggttt ccagcgaagt
    15061 gatcataaag accagcatca gcggcagcag cagactccat tcaatgccaa gaccgtaata
    15121 gagcggcgtt ggcaccataa tcagttcttg tgtcatcggt tcgttgcttt ctggcaacat
    15181 gcccataaac cacgccagcg catatccggc cgccatcgca atcaccagtg aggccacgcg
    15241 taagtaaggg ttacgttgac ggttaagcag gataattaag gctaagacca cgcctgccag
    15301 cagcagattt ttcggtgcgc cgaaggtgtt atcgctcatg gctgcgtaac cgccgccaat
    15361 ggaggtcagc ccaacctgaa ttagcgacag gccgataatc atcaccacaa cgccagaaac
    15421 cagcggcgta ataatgcggc gcgccagatg cagaacgcgg gagatcacca tctcggtaca
    15481 acttgccagc atcaacgtgc cgaacaaagc tgccatcatg gtaggaacat cagcaccacc
    15541 ggtcttcagc gctgtgccgc ccataatcag cggagcaaca aagttgaagc tagtgccctg
    15601 aatagacaac aatccggagc caaccggacc ccaggcctta atttgaataa tcgatgccac
    15661 accggaggca aacagcgaca tactaataat gtgttgcgtg tcttgtgccg gtaaacccag
    15721 cgcctggcag attaatagcg ctggcgtgat caccgcaacg aacatcgcca gcaaatgctg
    15781 acaggcggca aacagggttt gaggaagcgg cggacgatct tcaagacggt aaatcagttc
    15841 gctgttttga gtctgcgcaa ccggttgcgc attttctgac tcgagggtgg aaacagacat
    15901 cgaaaacaat cccgtggtag caaagcgggc attttagctg accgaaagag aaaagcaaac
    15961 gtttgccaca tcctcatcta aatatttaaa tgagataaat gtactttttt atataacttt
    16021 tagttgtttt ctgcttaaaa tccatgccat ttttctagtt tcagctaaga attcgaacca
    16081 ggaaagcatg actcgatact cgtcatattt cgccttactg caaatgcgcg atgaagtatg
    16141 acgagtatga aagagtgatg cggacacaaa ggagtaacta tgtttcatct cgatacttta
    16201 gcaacgcttg ttgccgcaac gctgacgttg ctgctcgggc gcaagttggt ccattccgtc
    16261 tcctttttga aaaaatacac cataccggaa cctgttgcgg gtggtttatt agtggcgctg
    16321 gcgctgctga tactgaaaaa aagcatgggc tgggaagtca actttgatat gtccctgcgc
    16381 gatccgttga tgctggcttt cttcgccacc attggcctga acgccaacct tgccagtctg
    16441 cgagctggtg ggcgtgtggt gggggttttc ctgattgtgg ttgttggcct gttggtgatg
    16501 caaaatgcca ttggcattgg tatggctagc ctgttagggc ttgatccgct gatggggctg
    16561 ctggccggtt ctattacgct ttccggcggt cacggtacgg gtgctgcatg gagtaaattg
    16621 ttcattgaac gttatggctt caccaacgcg acagaagtgg cgatggcctg tgcaacgttc
    16681 ggtttggtgc tgggcggctt gattggcggt ccggtggcgc gctatctggt gaaacactcc
    16741 accacaccga acggtattcc ggatgaccag gaagttccga ctgcgttcga aaagccggat
    16801 gtgggccgca tgatcacctc gctggtgctg attgaaacta tcgcgctgat tgctatctgc
    16861 ctgacagtgg ggaaaattgt tgcgcaactt ttggctggta ctgcttttga actaccgacc
    16921 ttcgtctgtg tactatttgt tggcgtgatt ctgagcaacg gtctgtcaat gatgggcttt
    16981 taccgcgtct ttgagcgagc ggtatccgtg ctgggtaacg taagcctgtc gttgttcctg
    17041 gcgatggcgc tgatggggct gaaactgtgg gagctggctt cgctggcgtt gccgatgctg
    17101 gcgattctgg tggtacagac catcttcatg gcgttgtatg ccatcttcgt tacctggcgc
    17161 atgatgggca aaaactacga tgcggcagtg ctggctgcgg gtcactgtgg ttttggcctc
    17221 ggtgcaacgc caacagcaat cgccaacatg caggcgatca ctgaacgctt tggcccgtcg
    17281 cacatggcgt tcctggtcgt gccgatggtc ggtgcgttct ttatcgatat cgtcaatgcg
    17341 ctggtgatta agctgtattt gatgttgccg atttttacgg gttaaccgat gaagcggcgg
    17401 tcttctcacc gccgcctttt acgcattcga gtagcgttct gtttctggca tccagcgttc
    17461 tatcagggct tttgcctgtt gtgggtaacg ttcgtgaata tgtcgcgcca ggcgctgaac
    17521 ttccgggatc atcgcctgat cgcgcagtaa atccgccact ttaaattcag cattccccgt
    17581 ctgacgtgta cctaacaatt cgccagggcc gcgaatctcc agatctttct gtgcaatcac
    17641 aaaaccatcg ttgctgtcgc gtagtacctg caggcggatt tgcgcggttt tggagagcgg
    17701 cgttttgtag agcagcacgc agtgagaggc caccgcgcca cgacccacgc ggccacgtaa
    17761 ctggtgtaac tgcgccagac ccagacgctc cgggttttcg ataatcatca gactggcatt
    17821 gggtacatca acaccgactt caatgacagt agtggcaacc agcaggtgta gctcgccttg
    17881 tttaaacgat gtcatcaccg cctgtttctc ggcgggtttc atccgtccgt gtaccaggcc
    17941 aacattcaac tctggtagcg ccagtttcaa ctcttcccag gtcgcttccg ccgcctgcgc
    18001 ttccagtaat tccgactctt caatcaacgt acaaacccag tacgcctgac gcccttcagt
    18061 catacaggcg tggcgcacgc ggtcaatgat atcgatacgg cgggtatcag gaatagcgac
    18121 cgtagtcact ggcgtgcggc ctggtggcag ctcatctatc accgaggtat cgagatcggc
    18181 atacgcagtc atcgccagcg tgcgggggat gggcgtggcg gtcatgatca actgatgcgg
    18241 atggaagccc tgctgctggc ctttctccca taacgccaga cgctgatgca cgccaaaacg
    18301 atgctgttcg tcgataatca ccagcgccag gccgttaaac tgcacctgtt cctggaaaat
    18361 ggcgtgcgta ccaacaatca tctgcacctg accgctggcg atggcttctt gctgagccag
    18421 tcgtgcttta cctttctgtt ttccggcgag ccagcctact tcgataccga gcggtgcaaa
    18481 ccagttacga aagttattgg cgtgctgctc ggcgagtaat tcggttggtg ccatcagggc
    18541 cacctgtttg ccgtgggcaa ccgcacgcaa cgcggcgagg gcggcgacca gcgttttacc
    18601 ggaacctaca tcgccctgca ccaggcgcat cattggcaca tccagcgcca tatcgcgctc
    18661 gatctccgcc actacgcgtg cctgtgcgcc cgttggcttg aacggtaagg cggcgaggag
    18721 tttatttttc agcgcgtcat tggtgctcag cggctgagca tgaaaacgtt gtgcaccggc
    18781 acgtaacgcc aacatgctga ggttgtgcgc cagtagttct tccagaatca gacggcgttg
    18841 tgccggatgc tgcccggttt cgagctcgct aagctgtagc gtcggcggcg ggcggtgcaa
    18901 agtgcgcaac gcttccggta gcgtcatcat tccttgtgac agttccggcg gcaggagttc
    18961 ttcaatggcg catgtgtcga gcagatccag cgcctggtcg gttaatttac gcagcgtagc
    19021 ctgctttacg ccttccgtag ttggataaac cggtgtgagc gtttcctgta actctggcgt
    19081 gctgagatcg ccctgtacgc ggtattccgg gtggatcatc tccgcaccat atttgccgcg
    19141 ctttgcttcg ccataggcca gcacgcggcg gcctgtcgcc aggctatttt tcattgccgc
    19201 attgaagttg aaaaagcgca tagtgagaat gccggaaccg tcgctgatct ggcaggtcat
    19261 catccgccga ccaccgaagg agatattgca gttcagcact tcgccttcca ccgtggcata
    19321 aacacccggc agtagttcac cgatggggta gagatgtgtg cgatcttcgt agcgcagagg
    19381 aaggtgtaag agcaaatcct gtacggtatg caggttgatt ttcgccagct tgttactaag
    19441 cgctgcgcca acgcccgtta gggaactgag cgggacagca tctaacaggc gacctttcat
    19501 cgcgcttacc ctgcggcctg catggtggac caccagtcag catcggcttc aatctcgcct
    19561 tgctgattaa cgtgggggta gggcagacct ttgcgttttg cgactttcgc cagcaccgga
    19621 tagccgcctt caaacagcag gcgttgttgc tcggcttccg gcaacatgct gttttcacgc
    19681 aggtacatgc ccgcattttg ccgctggcgc tgggcttcgt aaagaatgag ggctgaggca
    19741 acggaaacat tcagcgactg caccatgccg atcatcggaa tgatgatatc ctgatccgcc
    19801 agggccaacg cttcctgcgt gatgcccgtt ttctcctgtc ccatcaaaat acaggtcggg
    19861 cgagtgtaat caatttcgcg gaaatcaaca gcgttatcag aaagatgggt tgccagaatc
    19921 tgcatgccct ggcctttcag atgagcgacg gcatcgccaa tggtgcggtg cgttttcacc
    19981 tgtacccagc tgttactacc cgcagctgcc gaagccatgg tgcgcatccg gctgccaggc
    20041 cagacagcat gaacttcatg tacgccaacg gcatctgcgg tacgaataat cgcagaaacg
    20101 ttatgaggtt tgtggacctg ctccatgcag acggtcagat caggctgccg cctggcgagc
    20161 atttcgcaga tgcgtgcata acgtgttggg ttcataaaac attaatttcg gtttcgggtg
    20221 actttaatca cgtctggcat cacgcggatt ttgcgcatga tattcgccag atgcacacgg
    20281 tcacgggcgg tcagacgaat aaaggcgcta tagacgcgac catctttctc ttccgtattc
    20341 aaactttgaa tattcgaggt cgtggtgtta attgccgccg tcaggtttgc cagcgcgccc
    20401 tgatgattga acatctccac cttgatttcg gtgatgaatt cctgcgccgt ctctttatcc
    20461 cattcaacag ccataaactt ctctggctct ttctggtagc cacggatgtt acggcaggat
    20521 tcatggtgga tcaccagacc tttaccgggg ctgacgtggg cgataatcgg gtcgccagga
    20581 attgggcggc agcatttcgc aaaggtgatc agcacgccat cggcaccttt aatgggcaga
    20641 tgtccgtgac tttgggttgc cggtggaatg gaggcgtccc catgttgcag atttttcgcg
    20701 accaccacgc tcattgcgtt accaaggccg atttctgcca gcagatcgtc aagcgttgcc
    20761 agcttcatgc ggtccagctc gcgctgaata ttttcctgcg ggatttcatt gagcttacgg
    20821 ctaccaccca acgcatggtt gagcagacga cggcccaggc ttacagaatc atcacgcttg
    20881 aggtttttca gcaactgacg aattttggcg cgcgctttcg agctgacgac aaagttcagc
    20941 cacgcggcat tcgggcgagc acccggtgcg gtaatgattt caacggtttg accgctagta
    21001 agcggctgcg acagcgggta aggctggcgg tcgacgcgtg cgcccacgca ggcatggccg
    21061 atatcggtat gcaccgcata agcgaagtcg acgggcgttg caccggcagg cagttcgaca
    21121 atgcgccctt ctggtgtgaa aacgtaaatc tcatccggga agagatcgga tttaacgctc
    21181 tcgataaatt caaacgagct accggcactc tgttgcagct ccagcaggct ttgcatccag
    21241 cgctgggcgc gtatttgcgc ggtagtgctg gtttcgccgt gctctttata agcccagtgc
    21301 gcggcaacac ccatctccgc catctgatcc atatcttcgg tacggatctg gacctcaacc
    21361 ggcacgccgt gcgggccaat catcgaggtg tgcaaagact gatagccgtt cgcttttgga
    21421 atggcgatat agtctttcac gcggcccgga cgcggcttgt acaggctgtg catctggccc
    21481 agcacgcgat aacaggtgtc agaatcattg acgatcacgc ggaaagcgta gatgtccatg
    21541 attgagtgaa aacgctgctc tttgagcacc attttgcagt aaatcgaata aagatgcttt
    21601 tcgcgaccac tgacgcggca cggtattccc gcttcctgca aacgcccttc gatttcagaa
    21661 agaatcttct ggatcatctc cttacggttg ccgcgcgcgg ctttcaccac ttctttgatt
    21721 acgcgataac ggttgggata cagcgcctca aaacccagct cctcgagttc ggttttaatg
    21781 tggtggatac ctaaacggtg cgccagcggg ctgtatattt cgagagtttc acgggcgatg
    21841 cggcgacgtt tgtccgggcg aagtgagccc agcgtgcgca tgttgtgggt acggtcggca
    21901 agtttgatga gaatgacgcg gatatcctgc accatcgcca taatcatctt gcgaaagttt
    21961 tcggcctgcg cctctttctt atcgcggaac ttgagtttat caagtttcga caccccctct
    22021 accagctcgg cgacgctttt accaaaaagc tgttccatgt cctggtaggt ggcgggtgta
    22081 tcttcaatca catcatgcag cagcgccgcc atcagcgttt catagtcgag tttcatctcg
    22141 gccagaatgc aggcaaccgc caccggatgc gtgatatagg gttcaccgct tgaacgtgtt
    22201 tgcccctcgt gagcatcacg tgcaacgaga tacgcctgcc gcagacgctt gatttggtct
    22261 tccggcaggt agttttgaat cagctgattc aggctttcaa acagatacaa gggcgacccg
    22321 ctttgtgatt aacgacgacc ttcagcaata gcggtaacgg cttgtaattc agcggcttcc
    22381 tgctcttgct gttcctggcg ttcgcgaacg tcgaggatct ggttgttgat cagaccttct
    22441 tcgatttcgc gcagcgcgat tacagtggtt ttatcgtttt cttccggtac cagcggatcc
    22501 tttccgccta cctgcatctg acgagcgcga cgcgcggcga ccagtaccag gtcaaaacgg
    22561 ttaccaattt tctctacagc gtcctgaaca gttacgcgtg ccatacttaa aaagctccac
    22621 aggtgaagaa atgactgggc atgatactga aatcaggttc agtctgccaa caatttgctg
    22681 attaaagcgt catgacgctg cttttggcgg ctcatgcgca gacgttcggc gcgaataatg
    22741 gtcttcaaat cggtcaacgc ggtatcgaag tcatcattca caatcaggta atcatattcg
    22801 gcgtaatggc tcatttctgc aacagcttgc gccatacgct ttgcaatgac ctcttcgctg
    22861 tcctgaccgc gaccgcgtag acggcggtcc agttcgattt tagacggcgg taaaataaag
    22921 atactccgcg cgtgcggcat cttctggcga atttgctgcg caccctgcca gtcgatatcg
    22981 agaaaaacat cgacaccggt cgccagtact tgctcaatgg cctcacgcga agtgccatag
    23041 taattaccaa aaacttctgc gtgttcgagg aacgcatctc tactaatcat ttctttaaat
    23101 tcatcatgat taacaaagaa ataatgttca ccgtggactt caccaggacg cggttggcgt
    23161 gtggtgtgtg aaacagaaac ctgggtgtca tacaacggtt gggtttttaa taaagcctga
    23221 atcaggctgg atttacccgc gccactgggg gcagaaacaa tataaagcgt gccttgagcc
    23281 atgagtatct ttcgtatgtg attagcgaaa taaagcctac atacgagctt attatacacg
    23341 gcgcggccat gtgacgtagc ccttgctcac actttttgcg caaagttgtt gcattttgcg
    23401 tagctgcttc cgcatttgcc tgtgattcac tgcaataaag acgattctca ggaagcgtcc
    23461 tcgcagactc cgtttttgcc actcggcttt gtcttcacca agttgtatat ctgcctgact
    23521 atctacagcg gaggtgatga tgaaagtatg gatggcgata ttaataagta tcttgtgctg
    23581 gcaatcatcg gcgtgggcgg tctgtccggc ctggtcgcca gccagagcac aggaagaaat
    23641 ttcccgcctg caacagcaaa taaaacagtg ggacgatgac tactggaagg aagggaaaag
    23701 tgaggtggaa gacggtgttt acgatcagtt aagcgcccgt ctcacgcagt ggcaacgctg
    23761 ttttgggaac gagactcgcg atgtgatgat gccgccttta aacggtgcgg ttatgcatcc
    23821 ggttgcccat acgggtgtac gcaaaatggc ggataaaaac gcattaagtc tgtggatgcg
    23881 cgaacgtagc gatctttggg tgcagccaaa agttgatggc gttgcggtaa ccctggttta
    23941 tcgggacggg aaactgaaca aagcaatcag tcgcggcaat ggcctgaaag gcgaggactg
    24001 gacgcagaaa gttcgcttaa tttccgctgt gccgcaaacc gttagcgggc ctttagccaa
    24061 cagcacgctt cagggggaaa tatttctcca gcgtaagggg catatccaac aacaaatggg
    24121 gggaataaat gcccgcgcaa aagttgctgg cctgatgatg cgccagggga atagcgatac
    24181 gctaaactca ctggctgttt ttgtctgggc atggccggat ggaccgcatt taatgaccga
    24241 tcgtttaaag gatctggcta ccgcaggttt tactttaacg cagacgtata cccgcgcggt
    24301 taaaaatgct gatgaggttg cgcacgtacg caatgagtgg tggaaagcaa aattaccctt
    24361 cgtcaccgat ggcgtggttg tgcgagcggc gaaagaaccc gaatcccgtc attggctacc
    24421 gggccaggcg gagtggctgg tggcctggaa atatcaacct gtagctcagg ttgccgaagt
    24481 gaaggcgatt cagtttgcgg tgggtaagag cggtaaaata tcggtggttg cgtcacttgt
    24541 acctgtcatg ctggatgata aaaaagtcca gcgggtgaat attggttccg tcaggcgctg
    24601 gcaggagtgg gatattgcgc ctggtgatca gatcctcgtc agccttgccg gtcaggggat
    24661 tcctcgcatt gatgatgtgg tctggcgcgg agcagaacgt acaaaaccga caccaccaga
    24721 aaaccgcttt aactcgttga cctgctactt tgcttctgat gtttgtcagg aacagttcat
    24781 ttcacgctta gtctggctgg ggtcaaaaca ggttctgggg cttgatggca ttggtgaggc
    24841 cggctggcgc gccctgcatc agactcatcg ctttgagcat atcttttcct ggcttttatt
    24901 aacgccagag caattacaga acacgccggg gatcgcgaaa agtaaaagtg cgcagctatg
    24961 gcatcagttt aatctggctc gtcagcagcc ttttactcgc tgggtgatgg caatgggaat
    25021 accgctaacc cgggcggcgc ttaatgccag tgatgaacgg tcctggtcgc aacttttatt
    25081 tagcacggaa cagttctggc agcagctgcc ggggactgga tcggggcgcg ccagacaggt
    25141 tattgaatgg aaggaaaatg cgcaaatcaa gaagctgggc agttggctgg ctgcccagca
    25201 gatcacaggt tttgaacctt agtgaccttc gtggctgtag taaaagaccg gtaatcccag
    25261 cttaagacgt agcgccagta aacgggcaaa aaagccgaat accaaggtgg agataatcac
    25321 cacatcatgg ttagaaacat agtgttgcag cgcgatgtac agcacggcgg aggcaaatga
    25381 gacaccggca tacaactctt tctggaatac cagcgggatg cgtttacaga acatatcgcg
    25441 caaaacgccg ccaaacacgc cagtcgtcac agccgcgaca acggcaatga tcgggccgtg
    25501 tcccatatcc agcgcgacct gtgcgccgat gatagaaaag accaccagtc cgagtgcatc
    25561 gagcaccaga aacactttgc gcagataagg cattacagga gcaactatag tggtgagtac
    25621 tgcagcggta gcgacgataa tcacatattc cgggtgtttg acccagccga gcggatagtg
    25681 gcccagcaga atatcgcgca ctgaccctcc gccaattgcc gtggcggtcg caataataat
    25741 tacgccaaat gtatccatac gccgtcgtcc ggccgccagc gcccccgtca tggcttcggc
    25801 agtaatgcca accaaataca aaatgtgtaa cagcatggtt ctctccctaa aaatctgtct
    25861 ggaagggtag cgatttgtgc gcattgtcac gattgagatt ttttaaggag agaatagatt
    25921 gataatttgt agttatgtga aattgctatt ttctgtagta agaatgcatt tttctgattt
    25981 tgtgataagt aaaatgcatt agaaaaagta tggagtaaat aaatatgagg acgaaactat
    26041 atgcgtagcg ccgctgtaat taacaacggc ctacggagat atgtcgctta ctcaatattc
    26101 tgaatctgct cgcgcatctg ctcaatcaac actttcagct cgatggcgga gtttgtcact
    26161 tcggcgttga tagatttcga cgcaagagtg ttcgactcgc ggttgaactc ctgcatcata
    26221 aagtccagac gacgaccaac ggcttctttt ttcttcagaa tgttgtaggt ctctttgaca
    26281 tgcgcttcga ggcggtccag ttcttcggca acgtcaattc gttgtgccag cagaaccaac
    26341 tcctgctcca gacggttgtt ttccagttgc acctgagcat cttccagctt cgctatcaga
    26401 cgctcacgct gccattgcag gatttccggc atatgggcgc ggactttggc cacttcggcg
    26461 gtgacgcctt ccagacgctg ctcaatcaac actttcagtg cctgaccttc ggtttcgcgc
    26521 gcgatgataa agtcatccag ggtgccatcc aacgccgcga gaatttcagc tgcaatcgcg
    26581 tcgagatctt gctcctgggc tgccatcacg cccggccagc gtagaatatc aaccgggttg
    26641 atttctcctt cgtcgctctg catttttacc cagttcgcgg cagttaccag ctgtttagcc
    26701 agtttttcgt tgaggatcag ctcaccttgc gcgctaacat ctggctcata gcgcagagta
    26761 cattccactt taccgcgcgt caggcgagaa cgaatacgct cgcgaacgac aggttcaagg
    26821 ctacggaact gctccggcag acgaaagtaa gtttccagat aacgctggtt taccgagcgc
    26881 atttcccagg ttgcgctccc ccattcaccc ttgatttcac gccgggcgta ggcggtcata
    26941 ctgcggatca tagacgttcc tgtttataaa aggagaggtg gaaggattat agccatcgat
    27001 gccttgtcag gataggaata accgccggaa gtccgtataa tgcgcagcca catttgtttc
    27061 aagccggaga tttcaatatg cgtccagcag gccgtagcaa taatcaggtg cgtcccgtta
    27121 ccctgactcg taactataca aaacatgcag aaggctcggt gctggtcgaa tttggcgata
    27181 ccaaagtatt gtgtaccgcc tctattgaag aaggcgtgcc gcgcttcctg aaaggtcagg
    27241 gccagggctg gatcaccgca gagtacggta tgctgccacg ttctacccat acccgtaacg
    27301 cgcgtgaagc ggcgaaaggt aagcagggtg gacgcacaat ggaaatccag cgtctgatcg
    27361 cccgtgctct tcgcgcggca gtagatttga aagcactggg tgagttcacc attacgctgg
    27421 actgcgacgt gcttcaggct gatggtggca cgcgtaccgc ttcgattacg ggtgcctgcg
    27481 tggcgctggc agatgcgcta cagaagctgg tggaaaacgg caagctgaaa actaatccaa
    27541 tgaaagggat ggtagccgcg gtctctgtcg ggattgtgaa cggtgaagcg gtttgcgatc
    27601 tggaatatgt tgaagactct gccgcagaga ccgacatgaa cgtagtgatg accgaagacg
    27661 ggcgtatcat tgaagtgcag gggacggcag aaggcgaacc gttcacccac gaagagttac
    27721 tcaccttgtt ggcgctggcc cgagggggaa tcgaatccat tgtagcgacg cagaaggcgg
    27781 cgctggcaaa ctgattttta aggcgactga tgagtcgcct tttttttgtc tgtagaaaag
    27841 taagatgagg agcgaaggca tgaaaccata tcagcgccag tttattgaat ttgcgcttag
    27901 caagcaggtg ttaaagtttg gcgagtttac gctgaaatcc gggcgtaaaa gcccctattt
    27961 cttcaacgcc gggctgttta ataccgggcg cgatctggca ctgttaggcc gtttttacgc
    28021 tgaagcattg gtggattccg gtattgagtt cgatctgctg tttggccctg cttacaaagg
    28081 gatcccgatt gccaccacaa ccgctgtggc actggcggag catcacgacc tggacctgcc
    28141 gtactgcttt aaccgcaaag aagcaaaaga ccacggtgaa ggcggcaatc tggttggtag
    28201 cgcgttacaa ggacgcgtaa tgctggtgga tgatgtgatc accgccggaa cggcgatccg
    28261 cgagtcgatg gaaattattc aggccaatgg cgcgacgctt gctggcgtat tgatttcgct
    28321 cgatcgtcag gaacgggggc gcggcgagat ttcggcaatt caggaagttg agcgtgatta
    28381 caactgcaaa gtgatctcta tcatcaccct gaaagacctg attgcttacc tggaagagaa
    28441 gccggaaatg gcggaacatc tggcggcggt taaggcctat cgcgaagagt tcggcgttta
    28501 aagaaactcg ccggatgaaa agtcatccgg cgtcatatta ctgcaactgc gcggcaatta
    28561 gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt aaattcgctg cggacaaaac
    28621 gtgacagcat accttcacag aaggccagga tctggcttgc cagcagggtt tcatcggtgg
    28681 tgtaaccttc accctcacgc attctctttt cacgcaatac ctgacgcagc tgcgcttcaa
    28741 tacgctcgaa aagttggttg atgcgccctt gcaggcggtc ttgttcaaac atcagcgcat
    28801 gcccggtaag gatgcgggtc aggccaggat tacgctcacc aaaaccgaga agcagcaaca
    28861 caatcagacg caggcgtgct gtggtgtctt tctcatcttt cagaatcaga ttgatgcgag
    28921 taatcaggct atcttcgata aactcaatca ggctatcgaa catgcgggtc ttactgggga
    28981 agtggcgata cagtgccgct tcggaaacgc cgacagaggc ggccagtttt gccgtcgtga
    29041 tacgttggct tccatcgctg gattccagca tcagcgccag agactgaagt atttcctcgc
    29101 gacggttcct tttcgcagtt tgtttttctg ccatgttaca aaatacccct gaaaataagc
    29161 acttgccagg cggcacccac gctatgaccg caaacgaaat gtttgcggct atgttatgac
    29221 gttattcgga tgcgtatgtg ttactgacga ccagagtgac caaagccgcc ttcaccgcgg
    29281 tcggtggcgt cgaaatcttc caccagatta aattcagcct gtactaccgg aacaaaaatc
    29341 atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt cctgaccacg gttccacacg
    29401 gaaatcatca actggccctg atagtcagaa tcgatcaatc ctaccaggtt accaagcacg
    29461 ataccgtgct tatgtcccaa tccggagcgc ggcagcatca ttgccgccag tgaaggatcg
    29521 gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt cacccggagc cagttctacg
    29581 gcgtcgtcga gacaggcacg caggtcaagt ccggcagagc cagaggtggc ataagtcggg
    29641 agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa cgtcgatttt tttcatcata
    29701 acgggtcacg atctcgtcga gtaataattg gccaaggagc tctttgcgct caagcggtaa
    29761 gactttatct ccgtcctgcc agaaaaggtg taatgcgttg ttgtcgctgt taaatccttg
    29821 agttggctgg gaaacatcgt tcgcgcagat cagatcaagg tttttacgga tacgtttttg
    29881 ccgggcgtat tcttccacat tatttgtttc ggcggcaaat ccaacgacgt agggtcgatg
    29941 gtcttttagt gcggcaacgc ctgcgacgat atcggggttt ttaaccattt ttattgttaa
    30001 ttcatcaccc tgcgtggcct gctttttgat tttttctggg gccacggtag ctgcgcgata
    30061 atccgccacg gcggcgcaac cgataaaaat attttgctgc tgtacagaag cattcacggc
    30121 ggcttccatt tccagcgcgg tcatcacatc aacacggtta acaaacggtg gcgtcggtag
    30181 tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga cgggctgcgg cggcggcgat
    30241 agcaaaaccc atcttgccgg agctgtgatt agagatataa cgcacgggat cgagcggttc
    30301 acgcgtcgga ccggcggtaa tcataatgtt cagatgtttc aggtcgttga cgggcgaaaa
    30361 atgcgctacc gccatatcca caatagttaa cggatcgagc attcgtcctg ggccgatatc
    30421 accacaagcc tgactgccgc tgtctggccc ccagatgagc aaaccacgtg aggcaagcac
    30481 ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc tggttcatgg cggggagcac
    30541 ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc aggtcattcg ccattccggc
    30601 agcaacacgg gcaattaaat ctgccgtggc aggggcgaga atcactaaat cagcccattt
    30661 acccagctca atatggccca tagcggcttc ggctgccggg tccagcagac tgtcggaaac
    30721 gggataacca gaaaccgcct gcaagctaag tggggtgata aaggcttttg ccgcttcggt
    30781 catggctacg cggacatcgg ccccgcgatc gcgcaaacga cgcaccagtt caggggtttt
    30841 ataggcagca ataccgccgc taacgccgag aacgattttt ttaccggcca ggctcatgat
    30901 gatttttcct gttgggtgac accagaagtt ggcgatttta tcacaatact tttgttgtcg
    30961 tgccttcacc tgagattcac tttgcgaggc gctttccagg attgaaaact ggccgtcgat
    31021 ttaaaggaac ggctatgaca ggatgcgagc accacaaagg aggtgaaggt gaaaaacaat
    31081 gcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg gtattagcgt cttaacggat
    31141 gtcgagctgc tggcgctatt tctgcgtacc ggaacgcgcg gtaaagatgt attaaccctg
    31201 gcaaaagaga tgctggagaa tttcggctct ctttatggct tgttaacctc tgaatatgag
    31261 caatttagtg gcgttcatgg aattggcgtg gcgaaattcg cccagttaaa ggggattgcc
    31321 gaactggcgc ggcgttacta caacgtacgg atgcgtgaag aaagcccttt actcagcccg
    31381 gagatgacga gggaattttt acaaagccag ctcacgggcg aggagcggga gatctttatg
    31441 gtgatctttc tcgactccca acacagggtt ataacgcata gccgtctttt ttccggcacg
    31501 ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg aagcgataaa aataaacgcc
    31561 tcggcgctga tccttgcgca taatcaccct tcgggttgtg ctgaacccag taaagcggat
    31621 aaactcatta ctgaacggat aataaagagt tgtcagttca tggatttacg cgtgctcgac
    31681 catatcgtga ttgggcgtgg agagtatgtt tcttttgccg aacgcggctg gatttaaccc
    31741 gctatgcgcg atccttcggg atctttgtct gttcgggact tgagcacatc gctgagtcag
    31801 cgtatactac gccacctttg agaatctcgg gtttggcatt tgggcctggc aatcgagagt
    31861 tcacatagaa ctgcgatgac cgggctgtaa agcctgacga ggcgccaata ccccatacga
    31921 agctcgagct aatttgattt ttggagaata gacatgtccc gagtctgcca agttactggc
    31981 aagcgtccgg tgaccggtaa caaccgttcc cacgcactga acgcgactaa acgccgtttc
    32041 ctgccgaatc tgcactctca ccgtttctgg gttgagagcg agaagcgttt tgtcaccctg
    32101 cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag gcatcgatac agttctggct
    32161 gaactgcgtg cccgtggcga aaagtactaa gtacttagag gaaataaatc atggctaaag
    32221 gtattcgtga gaaaatcaag ctggtttctt ctgctggtac tggtcacttc tataccacta
    32281 cgaagaacaa acgtactaag ccggaaaaac tggaactgaa aaaattcgat ccagttgttc
    32341 gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt ctcgctttga tgtaacaaaa
    32401 aacctcgccc cggcggggtt ttttgttatc tgcttgcccc catattgact gcatctgttc
    32461 attcctggag atgctatgcc tgaattaccc gaagttgaaa ccagccgtcg cggcatagaa
    32521 ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc gcaacggacg cttgcgctgg
    32581 ccggtttcag aagagatcta ccgtttaagc gaccaaccag tgcttagcgt gcagcgccgg
    32641 gctaaatatc tgctgctgga gctgcctgag ggctggatta tcattcattt ggggatgtct
    32701 ggcagcctgc gcatccttcc agaagaactt ccccctgaaa agcatgacca tgtggatttg
    32761 gtgatgagca acggcaaagt gctgcgctac accgatccgc gccgctttgg tgcctggcta
    32821 tggaccaaag agctggaagg gcacaacgtg ctggcccatc ttggaccgga gccgcttagt
    32881 gacgatttca acggtgagta cctgcatcag aagtgcgcga agaaaaaaac ggcgattaaa
    32941 ccgtggctga tggataacaa gctggtggta ggcgtaggga atatctatgc cagcgaatcg
    33001 ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat cactgtcgct ggcggagtgt
    33061 gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt cgattgagca gggcggtaca
    33121 acgctgaaag attttctgca aagtgatggt aaaccgggct atttcgctca ggaattgcag
    33181 gtttacgggc gaaaaggtga gccgtgtcgg gtgtgcggta cgccgattgt ggcgactaaa
    33241 catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga agtaattgat gcgcgccgga
    33301 tggcatacca tccggcataa actctacgct aacttcgcca tcagcgcctg atggacattc
    33361 tccggcagga aatgggtgac atcgccctga tggcgcgcca cctctttcac caacgatgaa
    33421 gagataaacg accactcttt cgacggcatc agaaacacac tttccagctc cggcattaag
    33481 tggcgattca tatgcgccag ctgcatttca tattcaaaat ccgccaccgc acgcaggcca
    33541 cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg ccatcaaatc actaaacccg
    33601 accacttcca cgttccccag atgtgcggtt gcctgctgtg ccagttccac acgctcttcc
    33661 agggtaaaca tcggtttttt actggggcta gcggcaatcg ccagaataac gtgatcgaac
    33721 atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg taatgggatc gaaagtaccc
    33781 ggataaatcg cccgtttttg cataacaacc tcaatgcgtt ttcggtggca ggtaaggttc
    33841 cagcagttga agcagacgct gtagtgcgcc ctggttttga tacagcactt caacggcatg
    33901 acggccatag aaactacggt aatcggcgtc ggtgagtaaa gaggaaacct cttttgcaag
    33961 cgtagtggca tcggtaacgg taatcagccc gcttgcctgt tccagccgcg cgcaaatgtc
    34021 tttaaagtta aacgtatgcg gccccatcaa taccggaata gcgtgtgcgg cagcttccag
    34081 cggattatgc ccaccacgtt caaccagtga accgccaaca aaggcgagat cggcaatgcc
    34141 gtacagtaac atcaactcgc ccatcgtatc gccaaccaca acctgcgtgc tggtggaggg
    34201 gacttcccct gaagatcgtg tgatatagct tagtccagcc tggcggacaa ggttaatcgc
    34261 atccgggaag cgttccggat gacggggtac caggatgagc aataaattcg ggaattgctg
    34321 taacaatgcc tgatgtgcgg cgatcaccac actctcttcg ccttcatgag tgctggtggc
    34381 aatccatacc gggcggtgtg gtgcccattg gcggcgcagc gtcacggctt tagcagccaa
    34441 ctgcggcgtt acagaaatat cgaatttcag gctaccggta acggtcacct gattattttt
    34501 tgcgcccaac gccacaaaac gagcaccatc ttcttcattt tgggcggcaa tcagcgtaat
    34561 acgacgcagc aagcgacgga cgaatttacc cagtttggca taacctgcgg ccgagcgggc
    34621 agagagtcgc gcgttagcga tcaccagcgg aattttacgt ttatgtagcg ccgcaatcag
    34681 gttaggccat agttcggttt ccataatcaa caccagttta gggtcgactt tattcaggaa
    34741 acggttgagt gcatcgggca ggtcatacgg cagataaacg tgctgaacat ccttcccgaa
    34801 agccgattgt acgcgctccg aaccggttgg agtcatggtt gttacggtaa tcggtaaatc
    34861 aggataacga tgacgcagtg cgcgcaccaa cgggatcgcc gccagagttt caccgacgga
    34921 gacggagtgc agcataatgc cgcctggttt tagcggatgg cggtaaaaac cgtaacgttc
    34981 accccagcgt tttcgatagg ccggagcctt acgtccgcgc acccagagcc gtatccagat
    35041 cagcggctga ataaggtaga gaagggcggt gtaaagcaat tcgagcatag taaatagctg
    35101 acttatggat gtgctgggga ttctatgtat ttagctgtgg ctttaccatt acttttccca
    35161 tttttgactt aaatagcaat ttacccgcca attcttgctg aacatctata ctttttttgt
    35221 atttttatgc gactcattga tttctgcagt acgtgcagga aggaatcgca gagcaacagt
    35281 aaaagaatat ggttgctaat gattttattt gattgatttc tttattgaaa atcaacaatg
    35341 tgcttaaaaa tcattcgagt cggattgccg cgaaagaagt gatacactag cgcgtcaata
    35401 tcagtgcggt atcagtattc aggtagctgt tgagcctggg gcggtagcgt gctttttctg
    35461 cttcacctta atcggataat ctcaacaaaa agagttactt gtggataagc catttcgaag
    35521 aattttgctc attaagatgc gttttcatgg ggatatgtta ttaactactc ccgtcattag
    35581 ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg ttgctttatc aggacaccat
    35641 cccgatcctg tctgaaaatc cagagattaa cgcgctctac ggcataaaaa ataaaaaagc
    35701 aaaagcctca gaaaaaattg ccaacttttt tcatctcatc aaggtattac gtgccaataa
    35761 gtatgacctt atcgttaatc ttaccgatca atggatggtt gctatactgg ttcgcttatt
    35821 aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg cagtctgctt tttggcgtaa
    35881 tagtttcact catttggtgc cgttgcaggg tggaaatgtg gtggaaagta acttatccgt
    35941 gctgacgcca ttgggacttg aatcgttggt gaagcagaca accatgagtt acccgcctgc
    36001 aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt gttggacaaa attatgtggt
    36061 tatccaacct acggcgcggc aaatcttcaa atgttgggac aacgccaagt tttccgctgt
    36121 gattgatgcc ttacatgctc gtggttatga agtcgttctg acgtccggcc cagataaaga
    36181 cgatctggcc tgcgtcaatg aaattgcgca gggatgccag acgccaccag taacggcgct
    36241 ggccggaaag gtgaccttcc cggaacttgg tgcgttaatc gatcatgcgc agctgtttat
    36301 tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt aatacgccgc tgatatcgct
    36361 gtttggtgcg acagaccata ttttctggcg tccctggtca aataacatga ttcaattctg
    36421 ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt gaccgaaatg agatgtatct
    36481 ttcggttatt ccggcggcag atgtcattgc agctgtcgat aaattacttc cctcctccac
    36541 gacaggtacg tcgttatgat cgttgctttt tgtttatata aatattttcc ctttggcggt
    36601 ttgcagcgcg attttatgcg tattgctcag acagtcgccg cccgaggtca tcatgttcgg
    36661 gtttataccc agtcgtggga aggcgaatgc cctgatgtat ttgaactgat caaagtgccg
    36721 gttaaatcgc ataccaatca cgggcgcaat gcggaatatt ttgcctgggt gcaaaaacat
    36781 ttacgtgaac accccgtcga tagagtcgtt ggattcaaca aaatgccggg gctggacgtt
    36841 tattatgccg ctgatgtttg ttatgccgag aaagtagcgc aggagaaagg ctttttctat
    36901 cgcctgacgt cacgttatcg ccattatgcc gcctttgagc gggcaacctt cgaacagggc
    36961 aagccgacac agctgctgat gctgacagat aagcaaatcg ccgatttcca gaaacattat
    37021 cagactgaag cggagcgttt tcatattctg ccaccgggga tttatcctga tcgtaaatat
    37081 agccagcaac cagcgaatag ccgtgaaatc ttccgtaaga agaatggaat aaccgaacaa
    37141 caatatttat tgttgcaggt cggttcagac ttcacgcgta aaggtgtcga tcgttccatt
    37201 gaagcacttg cttcgttacc ggattcgctg cgccacaaca cattactata tgttgttggg
    37261 caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac gcggcgtgcg cagtaatgtt
    37321 cacttcttct cggggcgcaa cgatgtctcg gaattaatgg cggcggcgga tttattactt
    37381 catcctgcct accaggaagc cgcaggaatt gtactgctgg aagcgataac tgcaggatta
    37441 ccggtactaa caacagccgt ttgtggctat gcgcattata ttgtcgatgc caattgcggc
    37501 gaggctattg ctgagccatt ccgccaggaa acattgaatg agattttacg caaagcgtta
    37561 acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc gacattatgc tgatacacaa
    37621 gatttataca gtctgccaga gaaagcggcg gacatcataa cgggtggtct ggatggttga
    37681 acttaaagag ccgtttgcca cgttatggcg cggtaaagat ccttttgagg aagttaaaac
    37741 cttgcagggt gaggtatttc gtgaactgga aactcgccgc actctgcgct ttgaaatggc
    37801 gggcaaaagc tattttctca aatggcatcg cggcacgacc ctgaaagaga taatcaaaaa
    37861 tttactctca ttgcggatgc cagtattagg cgcagaccgc gaatggaatg cgattcatcg
    37921 actgcgggat gtcggcgttg atactatgta tggggtggca ttcggcgaaa aaggcattaa
    37981 tccgctgacc agaacctcgt ttattattac cgaagatctg acaccaacca tcagtctgga
    38041 agattactgt gctgactggg cgaccaaccc accagatgtt cgcgtaaagc gtatgcttat
    38101 taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg ggcattaacc accgtgactg
    38161 ttatatctgt catttcctgc tgcacttgcc tttttccggt aaggaagagg tgttaaaaat
    38221 ttcagtaatt gacctgcacc gggcgcagat acgtgcaaaa gtaccgcgtc gttggcggga
    38281 taaagatctt attggacttt atttttcttc gatgaatatt ggcctgactc agcgggatat
    38341 ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa gacattatca agcaggaaca
    38401 agggctgctg tcgcaagcag aagaaaaagc cacaaaaatc agggaaagaa cgattcgaaa
    38461 atcgttgtaa cccgtaactt atttttgcca aaattttgga tacagaataa atatgtct
//
LOCUS       DAZGJT010000037        37484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZGJT010000037 DAZGJT010000000
VERSION     DAZGJT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.037"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..3890
                     /gene="yfaL"
                     /locus_tag="ACHKFK_002066"
     CDS             132..3890
                     /gene="yfaL"
                     /locus_tag="ACHKFK_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO9052410.1"
                     /translation="MRIISLRKEYLSLLPSMIASLFSANGAAAVIDSCQGYDIKASCQ
                     ASRQSLSGNIQDWSIADGQWLIFSGMVNNASGGAVFLQHSAEFTISPQNETGMTLFAD
                     NSVSGEYNNGGAIFAKENSTINLANVIFDSNVAGGYGGAIYSAGTNDTGAADLRVTNA
                     VFHNNIANDGKGGAIYTINNDVYLSDDAFNNNQAYTSTSYSDGDGGAIDVTDNSTDNT
                     HLSGKTIINNTSFTNNYAEGYGGAIYTSSTTSPYLIDISVDDNYDQNNGVMIDENNSA
                     SGYDHSATAAAGGFMYIGHSVAEFNIAADKTLVIGNTSNDGAIDSLAGTGVIVKEGAG
                     ELVLNADNNAFTGEISIQNGEVTLGRSDELMNVGDTHCQSDPQDCFGLMVGSTVHSEY
                     QAELNVGNTQQTFVHSLTGFANGILNIDAGGNVTVNQGGFSGSIQGEGQLTVAQDGSY
                     LLTGAQSMALTGDIVVEDNAVLSLAGNQADLRAMQSDPQSIVLNGGVLDLSDFTTWDG
                     DSSYNDGLQISGSGGTVIGSNDVVDISSGDDLHIGGSDASQNGVYVVINAGDQRVTLA
                     NNNGYLGNTQIASGTLEVSDNSQLGNTSYNRSVIFTDPQQHSEMDVTTDVDTRSATTG
                     QGRNIEMRADGEIHVEDGVDTQWGGLMADSTGQQLDSVSTLTKSGGGTLELTASGTAT
                     SAVRVEDGTLKGEAENIIPYVSSLWVGEDGVFETGQNQDIRSIDATSGGDIDITDGTV
                     LRLTQQDTNQALDASLFSGDGTLVNATDGVTLAGELNTNLETDSLTYLSDVTVNGNLT
                     NTSGAISLQNSVAGDTLTVNGDYTGGGTLFLDSELNGDDSASDQLVLNGNTSGNTTVV
                     VNPITGIGEPTSTGIKVVDFAADPAQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWY
                     LRSQEVNPTPPPDPDPTPDPDPTPDPDPTPDPEPTPAYQPVLNAKVGGYFNNLRAANQ
                     AFVMERRDHAGGDGQTLNLRVIGGRYHYTAVGQLAQHEDTSTVQLSGNLFSGHWGDDG
                     EWMLGIVGGYSDNQGDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQRAWLDSWLQ
                     YVWFNNDVSEQDDGVDHYHSSGIIASLEAGYQWLPGHGVVIEPQTQVIYQGVQQDDFT
                     AANHARVSQSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDD
                     AVKQRGEIKVGITGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(4030..4752)
                     /gene="ubiG"
                     /locus_tag="ACHKFK_002067"
     CDS             complement(4030..4752)
                     /gene="ubiG"
                     /locus_tag="ACHKFK_002067"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO9052411.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHMTGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            4899..7526
                     /gene="gyrA"
                     /locus_tag="ACHKFK_002068"
     CDS             4899..7526
                     /gene="gyrA"
                     /locus_tag="ACHKFK_002068"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO9052412.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTSEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7675..9363
                     /gene="yfaA"
                     /locus_tag="ACHKFK_002069"
     CDS             7675..9363
                     /gene="yfaA"
                     /locus_tag="ACHKFK_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO9052413.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPKKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNVLMFATYQDKILAFSSSDMLFK
                     DDQQDTEATAIASDLLSGKKRWETRFGLEARTAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDASFDFTPVWNSMPAGASFCVAVPYSHGIAE
                     EMLSHISQENDKLNGALDGGAGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPVSQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQALNKTRPARMDVIPTDGIVPLYINPQGMAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9360..9983
                     /gene="yfaT"
                     /locus_tag="ACHKFK_002070"
     CDS             9360..9983
                     /gene="yfaT"
                     /locus_tag="ACHKFK_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO9052414.1"
                     /translation="MRHGLLVLICWLYCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANEALKVHDSKWLKSNGLSNQYLPPEMTLTPEQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYIIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            10007..14520
                     /locus_tag="ACHKFK_002071"
                     /pseudo
     CDS             10007..14520
                     /locus_tag="ACHKFK_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            14521..16170
                     /gene="yfaQ"
                     /locus_tag="ACHKFK_002072"
     CDS             14521..16170
                     /gene="yfaQ"
                     /locus_tag="ACHKFK_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO9052415.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDTLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWATGSGTSKTVLTRYATALNRVLPVPTQVASGQCVLVDLFARYPLKKVTEEKST
                     TAFKPGVLNGRYRVTFANGNHMTFVSHGETTLLTVKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTYLQQNADRDGDCLSIPDSSATQRVSASPATVGARTMTAWT
                     QDLIYAGDPVHYHGSRVTEGTLSWRHATAQAGQGERYDQILAFAYPDNNLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16175..16951
                     /gene="yfaP"
                     /locus_tag="ACHKFK_002073"
     CDS             16175..16951
                     /gene="yfaP"
                     /locus_tag="ACHKFK_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO9052416.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQTINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPEGQSRQKMQFYSTPGAGAIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(17025..18209)
                     /gene="atoB"
                     /locus_tag="ACHKFK_002074"
     CDS             complement(17025..18209)
                     /gene="atoB"
                     /locus_tag="ACHKFK_002074"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HFO9052417.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHVDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAQSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNIVTRKKTLVFSQDE
                     FPKVDSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMDESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     PEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(18240..19562)
                     /gene="atoE"
                     /locus_tag="ACHKFK_002075"
     CDS             complement(18240..19562)
                     /gene="atoE"
                     /locus_tag="ACHKFK_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HFO9052418.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SIVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVALIVVMPFITRMMMPKPSDVVSIDPK
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVMPAAQALGADLGKSVMAIAYG
                     EQWMNMAQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(19559..20209)
                     /gene="atoA"
                     /locus_tag="ACHKFK_002076"
     CDS             complement(19559..20209)
                     /gene="atoA"
                     /locus_tag="ACHKFK_002076"
                     /EC_number="2.8.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HFO9052419.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGILPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRRCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(20209..20871)
                     /gene="atoD"
                     /locus_tag="ACHKFK_002077"
     CDS             complement(20209..20871)
                     /gene="atoD"
                     /locus_tag="ACHKFK_002077"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HFO9052420.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYHLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(21067..22452)
                     /gene="atoC"
                     /locus_tag="ACHKFK_002078"
     CDS             complement(21067..22452)
                     /gene="atoC"
                     /locus_tag="ACHKFK_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HFO9052421.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSTSWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEAKTAPVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(22449..24275)
                     /gene="atoS"
                     /locus_tag="ACHKFK_002079"
     CDS             complement(22449..24275)
                     /gene="atoS"
                     /locus_tag="ACHKFK_002079"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HFO9052422.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGNRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKTLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSMI
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTAGLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILHQQTRDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISKLDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIRTWQYSDSQQAISIEDNGSGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            24442..27291
                     /gene="rcsC"
                     /locus_tag="ACHKFK_002080"
     CDS             24442..27291
                     /gene="rcsC"
                     /locus_tag="ACHKFK_002080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO9052423.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQTDVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLNKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(27491..28141)
                     /gene="rcsB"
                     /locus_tag="ACHKFK_002081"
     CDS             complement(27491..28141)
                     /gene="rcsB"
                     /locus_tag="ACHKFK_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO9052424.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(28158..30830)
                     /gene="rcsD"
                     /locus_tag="ACHKFK_002082"
     CDS             complement(28158..30830)
                     /gene="rcsD"
                     /locus_tag="ACHKFK_002082"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO9052425.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNENEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPEGKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETELFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            31569..32696
                     /gene="ompC"
                     /locus_tag="ACHKFK_002083"
     CDS             31569..32696
                     /gene="ompC"
                     /locus_tag="ACHKFK_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9052426.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNAPESENNSWTRVAFAGLK
                     FQDIGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGQNGSVSGENDPDFTGHGITNNGRKALRQNGDGVGGSITYDYEGFGV
                     GAAVSSSKRTWDQNNTGLIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLG
                     WANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVVAGRNYDDEDILKYVDVGATYY
                     FNKNMSTYVDYKINLLDDNQFTRAAGINTDDIVALGLVYQF"
     gene            32808..33863
                     /gene="apbE"
                     /locus_tag="ACHKFK_002084"
     CDS             32808..33863
                     /gene="apbE"
                     /locus_tag="ACHKFK_002084"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO9052427.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKNLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            33937..35001
                     /gene="ada"
                     /locus_tag="ACHKFK_002085"
     CDS             33937..35001
                     /gene="ada"
                     /locus_tag="ACHKFK_002085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO9052428.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYADASEALAAGFRPCKRCQPDKANPQQHRLDKITHACRLLEQETPVTL
                     ESLAEQLAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTQAILSAGFP
                     DGSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGSLSGY
                     RWGVSRKAQLLRREVENEER"
     gene            35001..35651
                     /gene="alkB"
                     /locus_tag="ACHKFK_002086"
     CDS             35001..35651
                     /gene="alkB"
                     /locus_tag="ACHKFK_002086"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO9052429.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGRLGWTTHRQGYLYLPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            35727..37370
                     /gene="yojI"
                     /locus_tag="ACHKFK_002087"
     CDS             35727..37370
                     /gene="yojI"
                     /locus_tag="ACHKFK_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO9052430.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSGSLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFILVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         9178 a         9178 c        10059 g         9069 t
ORIGIN      
        1 atctaaatta attatcagat aataacaaaa tagttattac atcaggggca gaacttacaa
       61 aagaaaaata ccctatatcg cataatgcga ttaaatacgc catcattata gaaaatgatg
      121 gttagatgaa tatgcggatt atctctctac gcaaggagta tttatcttta ctcccgtcaa
      181 tgattgcatc tcttttctct gctaacggtg ccgcagcggt cattgattca tgccagggat
      241 atgatatcaa agcgagctgt caggccagca ggcaaagcct ttcgggtaat atacaggact
      301 ggagcatcgc ggatggacaa tggttgattt tttcgggtat ggtcaataat gccagcggtg
      361 gcgcagtatt tttgcagcac agtgctgaat ttacgatatc accacaaaat gaaacaggga
      421 tgaccctgtt tgctgataac tcggttagtg gcgaatataa taatggtggg gctatctttg
      481 ctaaagaaaa ctcaacgata aatcttgcga atgttatttt cgacagtaat gttgcaggag
      541 gctatggcgg agcaatctat tctgccggta ccaatgatac cggtgccgcc gatttacgtg
      601 tcactaacgc cgtatttcac aataacatcg ctaatgacgg caaaggcggt gcaatttata
      661 ccatcaataa tgacgtctat ttaagtgatg atgcttttaa taataaccag gcatatacat
      721 caacaagtta tagtgatggg gatggcggcg ctattgatgt tacggataat agtacggata
      781 acacacatct ttcaggaaaa acgataatta ataatacctc ctttaccaat aactatgcag
      841 aaggttatgg cggagcaatt tataccagca gcaccacatc cccgtatctt attgatattt
      901 ctgttgatga taattatgac caaaacaatg gtgtgatgat tgatgaaaat aacagcgcct
      961 cgggatatga ccattcggcg acggcagcgg cggggggctt tatgtatatc ggtcatagtg
     1021 tggctgaatt taacattgcc gccgataaaa cgctggtaat aggcaatacc agcaacgatg
     1081 gcgcgattga ttctcttgcc ggaacgggag tcatcgttaa agagggagcg ggagagttag
     1141 tcctcaatgc cgacaataat gcgtttaccg gcgagataag tatccagaat ggcgaggtga
     1201 cgttggggcg cagcgatgag ttaatgaatg tcggcgacac gcactgtcag agcgatccgc
     1261 aggattgctt tggtctgatg gtcggcagca ctgttcattc tgagtatcag gcagaactga
     1321 atgttggcaa tacacaacaa acgtttgtgc actcattaac cggttttgct aatggcattt
     1381 tgaatatcga cgcaggcggc aatgttaccg taaaccaggg cggtttttcc ggctcgattc
     1441 agggtgaagg acagttgacg gtagcgcaag atggcagcta tctcctgaca ggggcgcagt
     1501 cgatggcatt aaccggcgat attgtggtgg aagataacgc cgtattgtcg ctggcgggga
     1561 atcaggccga tttacgggca atgcagagcg atccgcaatc gatcgtgcta aatggcggag
     1621 tattggatct ctctgatttc accacctggg atggcgatag ctcatacaat gacggtctgc
     1681 aaatcagcgg cagcgggggg acagtgattg gcagtaatga tgtcgttgat atcagcagtg
     1741 gcgatgattt acatattggc ggcagtgatg ccagccagaa tggcgtttat gtcgtcatta
     1801 atgcggggga tcagcgcgta acgctggcga ataacaacgg ttatctcggt aatacgcaaa
     1861 tcgcctccgg tacgcttgaa gtcagcgata attcgcaatt ggggaacaca agttacaacc
     1921 gttcagtgat cttcacagat ccccaacaac acagtgaaat ggatgtgacg actgatgttg
     1981 acactcgctc ggctacaaca ggtcagggca ggaatattga aatgcgcgct gatggcgaaa
     2041 tacacgttga ggatggcgta gatacgcaat ggggcgggtt gatggcggat agtaccgggc
     2101 aacaactgga tagcgtaagc acgttgacta aaagtggcgg cggtacgctg gagttgaccg
     2161 ccagcggcac tgcgacgtct gcggtacgtg tggaggacgg aacgctaaaa ggtgaagcgg
     2221 agaatattat tccttatgtt tcttcactgt gggtggggga agacggtgtt tttgaaaccg
     2281 ggcaaaatca ggatattcgt tcaatcgatg ccacctctgg cggcgatatc gacataaccg
     2341 atggtactgt gttgcgattg acgcagcagg atacgaacca ggcgctggat gcctcgctgt
     2401 ttagcggcga cggtacgctg gtgaatgcca ccgatggcgt gacgctggca ggtgagctta
     2461 ataccaacct tgaaactgac agcctgactt atctttccga cgtgacggtt aatggcaatc
     2521 tgaccaatac gtccggtgcg attagcctgc aaaatagcgt cgctggcgac acgctgacgg
     2581 taaacggtga ttacaccggt ggcggtacgt tgtttctcga cagcgaatta aacggcgatg
     2641 actcggcaag cgaccaactg gtgttgaacg gcaatacctc gggtaacacg accgtggtgg
     2701 ttaaccccat tactggcatt ggtgaaccga catctacagg tattaaagta gttgatttcg
     2761 cagctgatcc cgcgcagttt caaaacaatg cgcagttcag tctggcagga agcggctacg
     2821 tcaatatggg ggcgtatgac tacacgctgg tggaagataa caacgattgg tatctgcgat
     2881 cacaagaagt caatccgacg ccgccgcctg atccagaccc gactccagat cctgatccca
     2941 cgccggatcc cgacccaacc cccgacccgg aacctacgcc tgcctaccag ccggtgttga
     3001 atgccaaagt tggtggatat tttaataacc tgcgggcggc aaatcaggca tttgtgatgg
     3061 agcgacgcga tcacgctggt ggcgatggtc agacgctgaa tttacgcgtt atcggcgggc
     3121 gttatcatta cacggcggta gggcagttgg ctcagcatga agacacttct acggtgcagc
     3181 ttagcggcaa cctgtttagc gggcactggg gtgatgatgg cgagtggatg cttgggattg
     3241 ttggtggcta cagcgataac cagggcgaca gccgctcgaa tatgaccgga actcgcgccg
     3301 ataaccagaa ccacggttat gccgttgggc tgacctcaag ctggtatcag cacggtaatc
     3361 agaagcagcg ggcctggttg gatagctggc tgcaatatgt gtggtttaac aatgatgttt
     3421 ccgaacaaga cgatggcgtg gatcattacc actcgtcggg gattatcgcc tcgctggaag
     3481 ccggttatca gtggttaccg gggcatggtg tggtgattga accgcagacg caggtgattt
     3541 atcagggcgt gcagcaggat gattttaccg ccgctaacca tgcgcgcgtg tcacaatcgc
     3601 agggtgatga tattcagacg cggctgggtt tacacagcga atggcgtact gctgttggtg
     3661 tcacgccaac gttagatctg aattattatc acgatcccca tgcgacggaa attgaagaag
     3721 atggcagcac tatcagtgac gatgcggtga agcaacgggg tgaaataaaa gtgggaataa
     3781 cgggcaatat cagtcagcga gtttcgctgc gcggcagcgt ggcgtggcag aaagggagtg
     3841 atgattttgc ccagacggca gggtttttgt cgatgacagt gaaatggtaa tcgctgtctg
     3901 accggcactg ttgccggatg cggcgtaaac gccttatctg gcctacgggg agcatttgta
     3961 ggcctgataa gacgcggcaa gcgtcgcatc aggcttggcg cggttgccgg atgaatccaa
     4021 acaacaacct cacttattct gcgtatgcag catatagttc acatccacgc cggggccgag
     4081 cttaaaggaa ttagttatag ggttgtaatg cagcccggtc atatgccgtt ctttaagact
     4141 ggtctgatcc acccagccca gcaattctgc cggtttaata aacttcttca catcatgtgt
     4201 acctttaggc accatgcgca aaatatattc agcaccaacc accgccatca gccatgactt
     4261 gccgttgcgg ttaagcgtcg agaaaaagac atcgccgccc ggtttcacca gttgcgcaca
     4321 ggctctgacc actgactgag gatcggggac gtgctccagc atctccatac aggtcaccac
     4381 gtcatactgt ccggcatgtt ttgccgcgtg ctcttccacg gtttcctgca cgtaatccac
     4441 ctgaatgccg ctttccagtg cgtgcagttt tgccacctgc aatggctcaa agcccatatc
     4501 cagaccggtc accgtcgcgc cctcccgcgc catactctcg gccagaatgc cgccgccaca
     4561 accgacatcg agcacctttt tgccaaataa accgccagca cgctcggcaa tatagcccag
     4621 acgcagcggg ttaatgcggt gcagcggttt gaactcacct tcaagatccc accagcggga
     4681 ggcgacggct tcaaatttag cgatctcttc gtggtctacg ttgtggttta ccggcgattt
     4741 ttcggcattc attggcactt ctactccgta attggcaaga caaacgagta tatcaggcat
     4801 tggatgtgaa taaagcgtat aggtttacct caaactgcgc ggctgtgtta taatttgcga
     4861 cctttgaatc cgggatacag tagagggata gcggttagat gagcgacctt gcgagagaaa
     4921 ttacaccggt caacattgag gaagagctga agagctccta tctggattat gcgatgtcgg
     4981 tcattgttgg ccgtgcgctg ccagatgtcc gagatggcct gaagccggta caccgtcgcg
     5041 tactttacgc catgaacgta ctaggcaatg actggaacaa agcctataaa aaatctgccc
     5101 gtgtcgttgg tgacgtaatc ggtaaatacc atccccatgg tgacttggcg gtttatgaca
     5161 cgatcgtccg tatggcgcag ccattctcgc tgcgttacat gctggtagac ggtcagggta
     5221 acttcggttc catcgacggc gactctgcgg cggcaatgcg ttatacggaa atccgtctgg
     5281 cgaaaattgc ccatgaactg atggctgatc tcgaaaaaga gacggtcgat ttcgttgata
     5341 actatgacgg tacggaaaaa attccggacg tcatgccaac caaaattcct aacctgctgg
     5401 tgaacggttc ttccggtatc gccgtaggta tggcaaccaa catcccgccg cacaacctga
     5461 cggaagtcat caacggttgt ctggcgtata tcgatgatga agacatcagc attgaagggc
     5521 tgatggaaca catcccgggg ccggacttcc cgacggcggc aatcattaac ggtcgtcgtg
     5581 gtattgaaga agcttaccgt accggtcgcg gcaaggtata tatccgcgcc cgtgccgaag
     5641 tagaagttga cgccaaaaca ggacgtgaaa ccattatcgt ccacgaaatt ccttatcagg
     5701 tgaacaaagc gcgcctgatc gagaagattg cggaactggt aaaagaaaaa cgcgtggaag
     5761 gcatcagcgc gctgcgtgac gagtctgaca aagacggtat gcgcatcgtg attgaagtga
     5821 aacgcgatgc ggtcggtgaa gttgtgctca acaacctcta ctcccagacc cagttgcagg
     5881 tttctttcgg tatcaacatg gtggcattgc accatggtca gccgaagatc atgaacctga
     5941 aagacatcat cgcggcgttt gttcgtcacc gccgtgaagt agtgacccgt cgtactattt
     6001 tcgaactgcg taaagctcgc gatcgtgctc atatccttga agcattagcc gtggcgctgg
     6061 cgaacatcga cccgatcatc gaactgatcc gtcatgcgcc gacgcctgca gaagcgaaaa
     6121 ccgcgctggt tgcaaatccg tggcagctgg gcaacgttgc tgcgatgctg gaacgtgcgg
     6181 gcgacgatgc tgcgcgtccg gaatggctgg agccagagtt cggcgtgcgt gatggtctgt
     6241 actacctgac cgaacagcaa gctcaggcga ttctggatct gcgtctgcag aaactgaccg
     6301 gccttgagca cgaaaaactg ctcgacgaat acaaagagct gctggatcag attgcggaac
     6361 tgttgcgtat tcttggtagc gccgatcgtc tgatggaagt gatccgtgaa gagctggagt
     6421 tggttcgtga acagttcggt gacaaacgtc gtaccgaaat caccgctaac agcgccgaca
     6481 tcaacctgga agatctgatc acccaggaag atgtggtggt cacgctttct caccagggtt
     6541 acgtgaagta tcagccgctt tctgaatacg aagcgcagcg tcgtggcggg aaaggtaaat
     6601 ctgccgcacg tattaaagaa gaagacttta tcgaccgact gctggtggcg aacactcacg
     6661 accatattct gtgcttctcc agccgtggcc gcgtctattc gatgaaggtc taccagttac
     6721 cagaagctac tcgtggcgca cgtggtcgtc cgatcgtcaa tctgctgccg ctggaacagg
     6781 acgaacgtat caccgcgatc ctgccggtga ccgagtttga agaaggcgtg aaagtcttca
     6841 tggcgaccgc taacggtact gtgaagaaaa ccgtcctcac cgagttcaac cgtctgcgta
     6901 ccgccggtaa agtggcgatc aaactggttg aaggcgatga gctgatcggc gttgacctga
     6961 ccagcggcga agacgaagta atgctgttct ccgctgaagg taaagtggtg cgctttaaag
     7021 agtcttctgt ccgtgcgatg ggctgcaaca ccaccggtgt tcgtggtatt cgtttaggcg
     7081 aaggcgataa agtcgtctct ctgatcgtgc ctcgtggcga tggcgcaatc ctcaccgcaa
     7141 cgcagaacgg ttatggtaaa cgtaccgcag tggcggaata cccaaccaag tcgcgtgcga
     7201 cgaaaggggt tatctccatc aaggttaccg aacgtaacgg tttagtggtt ggcgcagtgc
     7261 aggtagatga ctgcgaccag atcatgatga tcaccgatgc cggtacgctg gtacgtactc
     7321 gcgtttcgga aatcagcatc gtaggccgta acacccaggg cgtaatcctt atccgtactt
     7381 cggaagatga aaacgtggtg ggtctgcaac gtgttgctga accggttgac gaggaagatc
     7441 tggataccat cgacggcagt gccgcggaag gggacgatga aatcgctccg gaagtggacg
     7501 ttgacgacga gccagaagaa gaataatttc atcgcttcat gccaaaaggg agccatctcc
     7561 cttttttgaa ttgaaaagtc caggctgtaa agtctgggct tttgtcgtat tagggcgcgg
     7621 taaagtttgg ctgtgcccgt aaaaaatggc tggctataca caaggaatgt ggcaatgagt
     7681 ggtgaaaaaa aggcgaaagg ctggcggttt tatggccttg ttggttttgg ggcaatagcg
     7741 ctgctatccg ctggcgtatg ggcgttgcaa tatgctggca gtgggccaaa aaaaacgttg
     7801 tcgccactgg tggtgcacaa caatctgcaa atcgatctca atgagccgga cctctttctc
     7861 gacagcgact ctctgagcca gcttcccaaa gatctcctta ccattccgtt tctccacgat
     7921 gttctgagcg aagatttcgt tttctattat cagaatcatg ccgatcgtct gggcattgaa
     7981 ggcagcattc gccgcattgt ctatgaacac gatctcacgc tgaaagataa gctcttttcg
     8041 tcactcttag accagcccgc gcaggcggca ctgtggcatg ataaacaagg tcatctttca
     8101 cattatatgg tgcttatcca gcgcagtggt ttaagcaaac tgctggagcc attgttgttt
     8161 gccgctacca gcgacagcca gttaagcaag acggaaatca gtagcatcaa gataaatagt
     8221 gaaactattc ctgtttatca gttgcgctat aacggcaata acgtcctgat gtttgcgact
     8281 tatcaggaca aaatattggc gttttccagt tcggatatgc tgtttaaaga tgaccaacag
     8341 gataccgaag ctacggcgat cgcaagtgat ttgttgagtg gtaaaaaacg ctgggaaacc
     8401 cgctttggcc tggaagcgcg tactgccgaa aaaacgccag tacgccagcg catcgtagtc
     8461 agcgccaggt tgctggggtt tggctaccag cgattaatgc cttcttttgc tggcgtgcgc
     8521 ttcgaaatgg gtaacgacgg ctggcatagc tttgtggcgt taaatgatga atccgccagc
     8581 gtagatgcca gtttcgattt tacgccggta tggaacagta tgcctgccgg agccagcttc
     8641 tgtgtggcgg tgccgtattc acacggtatt gccgaagaga tgctttcgca catcagccag
     8701 gaaaacgaca agctgaatgg ggcgttagac ggtggcgccg ggctgtgctg gtatgaagac
     8761 tcaaaattac aaaccccgct atttgttggt cagtttgatg gcactgccga acaggcgcaa
     8821 ttgccaggga aactgtttac gcaaaatatt ggtgcgcacg aaagcaaagc gccagaaggt
     8881 gttttgccgg taagccagac tcagcagggc gaagcgcaaa tctggcgtcg cgaagtgagt
     8941 tcccgatacg gtcagtatcc gaaagcgcag gcggcgcaac ccgatcaatt aatgtcggat
     9001 tattttttcc gcgtgtcgct ggcgatgcaa aacaaaacgc tgcttttctc cctcgatgac
     9061 acgctggtta ataacgccct gcaagcactg aataaaaccc gcccggcaag gatggatgta
     9121 atacccactg atggcatcgt tccgctctat atcaatccac aaggaatggc gaaactgctg
     9181 cgtaacgaaa cgctgaccag tctgccgaag aatctcgaac cggtttttta taacgccgca
     9241 caaactttat taatgccgaa gctggacgct ttatctcaac aaccgcgtta tgtcatgaag
     9301 ctggcccaga tggaacccgg tgccgcctgg cagtggctac ccataacctg gcaaccgcta
     9361 tgagacacgg gctgctggtg ctgatttgct ggctgtattg tgttgttgcc catagtgaaa
     9421 tgctaaatgt cgaacaatcc gggctgtttc gcgcgtggtt tgtgcgcatt gcacaggaac
     9481 agctccgcca ggggccaagc ccacgctggt atcaacagga ttgcgcgggc ctggtgcgat
     9541 ttgcggcgaa cgaggcgctg aaagttcacg acagtaaatg gctaaaaagt aacggtttat
     9601 ctaaccagta tttgccgcca gagatgacgc taacacctga acagcgtcaa ctggcgcaaa
     9661 actggaatca ggggaatggg aaaaccggcc cctatgtgac cgcgattaat ttgattcagt
     9721 acaacagcca gtttattggc caggacataa accaggcact gcctggcgat atgatttttt
     9781 tcgatcaggg cgatgcccag cacttaatgg tctggatggg gcgttacatc atctaccaca
     9841 ccggaagcgc cacgaaaact gacaacggaa tgcgcgcagt cagtctgcaa caacttatga
     9901 catggaagga cacccgatgg atacccaacg attccaatcc caatttcatt ggcatttatc
     9961 gtttaaattt tctggcgcga tagcagcgtg cttgtccctg tctcttgtgg gaacagggct
    10021 tgctaatgct gatgattcgc ttccttccag taactatgtg ccgcccgccg ggggaacatt
    10081 ctttttgctc gctgacagca gttttagcag cagtgaagag gcgaaagtgc gactggaagc
    10141 gccggggcgt gattatcggc gctatcagat ggaagagtac ggcggcgtgg acgttcgcct
    10201 gtatcgtatt cctgacccga tggcattttt gcgccagcag aaaacctgca tcgcattgtg
    10261 gtgcaaccgc aatatctggg cgacggactg aacaatacgc tgacctggct gtgggataac
    10321 tggtacggca aatctcgccg cgtgatgcag cgtactttct cttctcagtc acggcagaat
    10381 gtgactcagg cgttacccga attacagctc ggtaatgcca ttattaaacc ttcccgttat
    10441 gtacagaaca accagttttc accgctgaaa aaatatccac tagtggaaca gttccgttac
    10501 ccactatggc aggctaaacc ggtcgagccg caacaagggg taaaactgga aggcgcatcc
    10561 agcaatttca tttcgccgca gccgggcaac atctatattc ctctcggcaa acaagagccg
    10621 ggactgtacc tcgtcgaggc gatggtgggt gggtatcggg cgacgacggt ggtgtttgtt
    10681 tccgataccg tggcgcttag caaagtgtca ggtaacgagc ttctggtgtg gaccgcgggg
    10741 aaaaaacagg gtgaagcgaa gcccggctca gagatcttgt ggactgacgg tcttggcgtg
    10801 atgacctgcg gtgtgaccga tggcagcggt accttgcagt tacaacatat atcgccagaa
    10861 cgttcatata ttctgggtaa ggatgctgaa ggcggcgttt ttgtctccga gaactttttc
    10921 tacgaaagcg aaatctacaa cacccgcttg tatatcttta ccgatcgccc gctatatcgc
    10981 gcaggcgatc gtgtcgatgt taaagtgatg ggccgcgagt tccacgatcc gttgcattca
    11041 tcccccatcg tcagcgcacc ggcgaagctt tcggtgctgg acgccaacgg cagtctgttg
    11101 caaaccgtca atgtcacgct ggatgcgcgt aatggcggac aggggagttt ccgcctgcca
    11161 gaaaatgccg tcgccggagg ttatgagtta cgtcttgctt accgcaaaca ggtctatagc
    11221 agcagttttc gcgtggcaaa ctacatcaaa ccccattttg agattggttt agctctcgac
    11281 aaaaaagagt tcaaaactgg cgaagcggtc agcggcaaac tgcaactgct ttacccggat
    11341 ggcgagccgg taaaagatgc ccgcgtgcag ttaagtttgc gcgctcagca attatcaatg
    11401 gttggtaacg atttgcgtta tgccggacgt ttcccggtgt tgctggaagg cagtgaaacg
    11461 gtttccgacg ataatggtca tgtggcgttg aatctccccg ccgccgataa accgagccgt
    11521 tatttgttaa ccgcctccgc cagtgacggc gcggcgtatc gagttaccac caccaaagag
    11581 atcctcattg aacgtggcct ggcgcattac tctttaagta ccgccgcaca atacagtaat
    11641 agcggcgagt cggttgtgtt ccgttatgcc gcgctggaat cttcaaaaca ggttcctgtt
    11701 acgtatgaat ggttgcgtct cgaagaccgc acgagtcaca gcggagagct acagtcaggc
    11761 ggcaaatcct ttaccgtcaa tttcgctaaa cctggcaact acaatctgac attacgcgat
    11821 aaagacggct taattctcgc cgggttaagc catgcagtca gcggtaaggg cagcacgtcg
    11881 catactggta cggtagatat cgtggcagat aaaacactgt atcagcctgg cgaaaccgcg
    11941 aagatgctga ttaccttccc ggagccaatt gacgaagcat tattgacgct ggaacgcgat
    12001 cgcgtggaac atcagtcgct gctatcgcat ccggcaaact ggctaacgct acaacgttta
    12061 aacgataccc agtatgaagc ccgcgtaccg gtgagcaatt cctttgcgcc taacatcact
    12121 ttttcggtgc tgtatacccg taacggtcag tacagttttc agaacgccgg gattaaagtt
    12181 gccgttcccc agctggatat ccgggtgaaa acggacaaaa cccattacca gcctggtgaa
    12241 ctggtcaatg tcgaattaac ctcgtcgctg aaaggtaaac ctgtttctgc gcagttaacg
    12301 gtaggcgtgg tcgatgaaat gatctacgcg ctgcaaccgg aaatcgcgcc gaatatcggc
    12361 aaatttttct atccgctggg gcgtaacaat gtgcgtacca gctccagtct gtcgtttatc
    12421 agctacgatc aggcgctctc cagcgagccg gttgcgcctg gcgcgactaa ccgcagcgag
    12481 cggcgagtaa aaatgctgga acgtccacgg cgtgaagagg tggataccgc ggcctggatg
    12541 ccgtcactca caaccgataa acaaggcaaa gcgtatttca cgttcctgat gcctgattcg
    12601 ttaacccgct ggcgcatcac cgcgcgtggg atgaacggcg acgggctggt cgggcagggg
    12661 cgtgcttatc tgcgttcgga aaaaagtctc tacatgaaat ggagtatgcc aacggtgtat
    12721 cgcatgggcg acaaaccggc ggcaggactg tttatcttca gtcagcagga taacgaaccg
    12781 gtggcgctgg tgactaaatt tgcaggtgct gagatgcgcc agacgctgac gctgcacaaa
    12841 ggggcgaatt atatttcgct gacgcagaat attcagcaat ctggcttgtt aagtgcagaa
    12901 ctgcaacaaa atgggcaagt gcaggacagc attagcacaa aactgtcttt tgtggacaac
    12961 agctggcccg ttgaacagca gaaaaatgtc atgctcggcg gtggcgagaa cgcgctgatg
    13021 ttgcccgagc aggcgagcaa tatccggctt caaagtagtg aaacgccgca ggagattttc
    13081 cgcaacaatc ttgatgcgtt agtcgatgaa ccgtggggtg gggtgatcaa caccggtagc
    13141 cgtctgatcc cgctcagtct cgcctggcgt tcgcttgccg atcatcaaag tgccgccgct
    13201 aacgacattc gtcagatgat tcaggataac cgtctgcggc tgatgcaact ggcagggccc
    13261 ggtgcgcgct ttacctggtg gggtgaagat ggcaatggtg acgccttcct cacggcatgg
    13321 gcatggtacg ccgactggca ggccagccag gcgctcggcg taacgcagca accggaatac
    13381 tggcagcata tgctcgacag ctacgcggag caggcggata acatgccgtt attgcatcgg
    13441 gcgctggtgc tggcgtgggc gcaggagatg aatctgccgt gcaaaacgtt gttgaaaggg
    13501 ttggatgaag ctatcgcccg gcgcggaact aaaactgaag atttctctga ggaagacacc
    13561 agcgatatca atgacagcat gatcctcgat acacctgaat ctccactggc agatgcggtg
    13621 gcaaacgtct taaccatgac gttgctgaaa aaagcgcagt tgaagtccac tgtgatgcca
    13681 caggttcagc aatatgcgtg ggataaagca gcaaacagca accaaccgct ggcgcacacg
    13741 gttgtgctgc tcaatagtgg aggcgacgct acccaggcgg ccgctatttt aagtggttta
    13801 accgctgagc aatccaccat tgagcgcgcg ttggccatga actggctggc gaaatatatg
    13861 gcgacaatgc ctccagttgt gttgcctgcg cctgcgggcg catgggccaa acataagtta
    13921 accggaggag gcgaatactg gcgttgggtt ggtcagggcg tgccggacat tctctctttt
    13981 ggtgatgaat tgtcgccgca aaatgtgcag gtccgctggc gtgaaccggc aaaaacggct
    14041 caacaaagta acattccggt gaccgttgaa cgccagttgt atcggcttat ccccggtgaa
    14101 gaagagatga gctttactct gcagccggtg accagcaatg agattgacag cgatgcgctg
    14161 tatctcgatg aaattacgct taccagcgag caggatgccg ttctgcgcta tggtcaggtg
    14221 gaagtaccgc tgccgccggg ggctgacgtt gagcggacaa cgtggggcat ttcggtcaat
    14281 aaacctaacg ctggaaaaca gcaggggcaa ttgctggaaa aagcgcgtaa tgaaatgggc
    14341 gaactggcct atatggtgcc ggtgaaagaa ctgacgggaa cggtcacttt ccgccatttg
    14401 ctgcgcttct cgcaaaaagg gcaattcgtt ctgcctcctg ctcgttatgt gcgttcctat
    14461 gcacctgcac agcaaagtgt tgcggcaggg agtgaatgga ccgggatgca ggtgaaataa
    14521 gtgaactggc gcagaatcgt ctggctattg gcgttggtta ctctgccaac gcttgcggag
    14581 gagacgccgt tacaactggc gctgcggggc gcgcaacacg atcaactgta tcagctgtcg
    14641 tcatcaggcg tgaccaaagt ctcggcatta cctgacacgc tcaccacgcc attagggagt
    14701 ttgtggaaac tgtatgttta cgcctggctg gaggataccc atcaaccgga gcagccctac
    14761 cagtgtcgcg gaaactcccc ggaagaggtt tattgttgtc aggctgggga gagcataacg
    14821 cgcgataccg cgctggtacg ctcctgtgga ctctattttg cgccgcagcg gttgcatatt
    14881 ggcgcagatg tgtgggggca atactggcaa cagcgtcagg cacctgcgtg gttagcgtct
    14941 cttactacgc tgaagccaga aacctcggta acggttaaat cgctgcttga ttctttagcg
    15001 acgttacccg cgcaaaacaa agctcaggaa gtcttactgg atgtggtgct ggacgaggcg
    15061 aaaatcggtg tcgcatccat gctcggtagt cgggtacggg tgaaaacctg gagctggttt
    15121 gccgatgata agcaggaaat ccgtcagggt ggttttgctg gctggttaac cgatggtaca
    15181 ccgctgtggg ctactggcag tggcaccagt aaaacggtac tgacccgtta tgcgacggcg
    15241 cttaatcggg ttttgccagt gccgacgcaa gttgccagtg ggcaatgtgt gctggtggat
    15301 ctgtttgctc gttatccgct gaagaaagtt actgaggaaa agagtacgac ggcattcaaa
    15361 ccaggcgtgc tcaatgggcg ctatcgtgtg acgtttgcca atggtaacca catgacattt
    15421 gtcagccacg gtgagacaac gttactgacg gtaaagggca aactgaaatt acagtcgcat
    15481 ctcgatcgtg aagagtacgt tgccagagtt ctggatcgcg aggcgaaaag tacgcctcct
    15541 gaggcagcga aagccatgac cgtggcgatt cgcacgtatt tacagcaaaa cgccgatcgc
    15601 gatggtgatt gcctgagcat cccggacagt agcgctaccc agcgcgtttc agcttcgcca
    15661 gccactgtag gcgcgcggac gatgaccgcc tggacgcagg atctcatcta tgcgggcgat
    15721 ccggttcatt atcatggcag ccgggttacc gaagggacgc tttcctggcg gcacgctact
    15781 gcccaggcgg ggcagggaga gcgttatgat cagatacttg cgtttgctta tcccgacaac
    15841 aaccttagcc gctggggagc gccacgctca acctgccagt tattacccaa agcgaaagcc
    15901 tggctggcga aaaaaatgcc gcagtggcgg cgtatattac aaggtgagac gggatacaac
    15961 gaaccagacg tgtttgcggt ctgtcgttta gtctcaggtt tcccctatac cgatcggcag
    16021 cagaaacggc tgtttattcg taatttcttt acgcttcagg atcggctgga tttaacccat
    16081 gagtatctgc acctggcctt tgatggttat cccaccgggc ttgatgagaa ctatatcgaa
    16141 acgctgaccc gacaattatt aatggactaa tgctatgcga aagatttttc ttccgttgtt
    16201 actggtggcg ctttcgcctg tcgctcacag tgaaggtgtg caggaagtcg agattgatgc
    16261 accgctctct ggctggcatc cggcagaggg tgaagatgcc agtttttcac aaacgatcaa
    16321 ttacccggcg tcgtccgtca acatggcaga tgatcaaaat atctcagcgc agatccgcgg
    16381 caaaataaag aattatgctg cggcgggtaa agttcagcag ggccggctgg tggtcaacgg
    16441 tgccagtatg ccgcagagaa ttgaatcaga tggttctttt gcacgccctt atattttcac
    16501 tgaaggcagc aacagcgtgc aggtcatcag cccagagggg caaagccgac aaaaaatgca
    16561 gttttactca acgccgggtg ctggggcgat tcgcgcacgt ttacggctgg ttctctcgtg
    16621 ggatacggac aataccgacc tcgatcttca tgtcgttacg cctgacggtg aacatgcctg
    16681 gtacggtaac accgtgctga aaaacagtgg tgcactggat atggatgtca cgacagggta
    16741 cggccctgag attttcgcca tgccagcgcc agttcacggc cgttatcagg tgtatatcaa
    16801 ctactatggc ggacgcagcg aaacggaatt aaccaccgcc caactgacgc tgatcaccga
    16861 tgaaggatcg gtcaatgaga aacaggaaac atttattgta ccgatgcgta atgctggcga
    16921 gctgacgctg gtgaaaagtt ttgactggtg attaatgggg ttaccttcgc cgtcgatgtt
    16981 caagaaaacg cccgataact ttcgctatcg ggcgttttta ttggttaatt caaccgctca
    17041 atcaccatcg caattccctg gccgccacca atgcacagtg ttgccagccc cagcgtttta
    17101 tcgcgtgcct gcattgcatg taatagtgtg accagaatac gagcaccact ggcaccgata
    17161 ggatgcccca gcgcgatggc cccgccgttg acattcactt tctcaggatc aaagcccagg
    17221 gttttcccaa cggcaaggaa ctgtgcagca aatgcttcat tagcctcaat gagatcaata
    17281 tccgccagtt gcagccccgc cagttgtaac gctttttgcg tggcaggtac tggccccata
    17341 cccatcaatg cggggggcac gccaccgctg gcataacttt taatgcgagc caggggggta
    17401 aggcctgctg ccagcgccgc agattcatcc ataatcacca gagcggcagc accgtcgtta
    17461 atgccagacg cgttcccggc ggtgactgtt cctgctttat cgaaggccgg gcgcaatgcg
    17521 cctaacgctt cagccgtaga atccactttc gggaattcgt cttgactgaa gacgagggtt
    17581 ttcttccggg tgacaatatt taccgggacg atttcggctg taaaagcacc ggactcaatt
    17641 gcggctgcag ctttacgctg tgaatgtagc gccagttcat cctgcatttc acgggtaatt
    17701 ccgtactctt tagccacgtt ttcggcggta atccccatat gataaccatg ggtggcgcac
    17761 atcaggccat cgcgcaggat tacgtcataa acctgtccgt ctccaagacg ataaccagag
    17821 cgtgcttttg catcgagtaa gtagggcgct aaactcatat tttccatacc ccccgccaca
    17881 atgctctgtg cctgacctgc ctgaatcgcc tgtgcagcaa gcgccacgct tttcagacct
    17941 gaaccgcaca ctttgttgac cgtgaatccg cacaccgttt cagccagtcc gctttttagc
    18001 agcgcctgac gtgccggatt ttgccccagt ccggcttgca acacgttacc cataatcact
    18061 tcatcaacgt gtagtgaatc gatttttgca cgttcaatgg cggctttaat tactgtcgcc
    18121 cctaggtcga tggcgctggt ggacgcgagt gaaccgttaa aactaccgat agcagtacgt
    18181 accgcactga cgatgacaca atttttcatt ttatattcct tctgtttgta gggttgccgt
    18241 cagaacagcg ttaaaccaat gacgaaaatg acgccggaga aaagcagggc agtgatgcaa
    18301 tagcccatga tgtcgcggac accgagtccg gcgattgcca gtgctggcag cgcccagaac
    18361 ggttgtgcca tgttcatcca ttgctcgccg taggcgatcg ccattaccga tttaccgaga
    18421 tcagcgccca gcgcctgggc tgcgggcatc acgaacggcc cctgaataac ccagtgcccg
    18481 ccgccagacg gaacggcgaa gttaatcagt gcagaactaa aaaaggtcat gaccgggaag
    18541 gtgtctttgt tcgcaacatt gatgaagaat tcggtaatga gtccgcccag accggagtgc
    18601 tccatcatca gttggatccc cgcgtagaag gggaattgca ccagaatacc ggcggtactg
    18661 cgtgctgccg cgctgatagc acgcatataa gccattggcg ttttatgtag cagcagaccc
    18721 gcaatcataa acatcaggtt gacggtattg atggtgatgt tgaagccatg ttcgctgaag
    18781 tacatcgcca ggtaagcgat accgagtgcg ccgatgatca acgtcagaat gcggctttct
    18841 tccagtcgct cggacggtgg agcatctttc ggtagttgct tttgaaaatc agcctcttcc
    18901 atgagtagtt tcggatcgat actcaccacg tcagacggtt ttggcatcat catgcgggtg
    18961 ataaatggca tcaccacaat caacgccaca gtgatgaaaa tattaaaacc actgaacaga
    19021 gtatcgccca ccgggatcag cccggcgata tgctcaaccg ggttgcccgg tgttgcagcc
    19081 aacagaggca ttgagccaga gaaaccgcca ccccaggtga gaaaaccaat gtaggcgcag
    19141 gcaatgagca acggataatc agaaccgggt actcgccggg cgacttcacg ggcgaacatt
    19201 gcaccgacaa ccaaaccaaa tccccagttg atgacacaag caactgaacc gaagaaagta
    19261 accagcatga cgccctgtac gggcgtcttt gcggcagagg cggcagtacg cagcaaactt
    19321 ttcaccggag cggagctggc aagggcatga ccggtaacga tgataagcgc catctgcata
    19381 ccaaacgcca gcaagttcca gaaaccgtca ccccacattt tcactatgct gatcggcgtt
    19441 tgtggtgtta accaaagcgc gatcacgaat gttagcaatg tcagcaacat ggcaaagatc
    19501 agtggatcgg gaagccaccg gctgacaaaa cgcgtcataa aacgcgatat gcgaccaatc
    19561 ataaatcacc ccgttgcgta ttcagatcgg cagctacttc aaaccgagct tctgttttgg
    19621 cacgcacggt ggctaaatca cacccgtcgg caatttcggt gagccacatt ttgccgtcaa
    19681 taaaacgaaa gacagccagt tcagtaacca gcatatgcac cgcatgttgc gcagtgagtg
    19741 gcatggtgca gcggcgcaaa atttttgctg aaccatcttt ggcgcaatgt tccatggcga
    19801 tgatcacttt gcgcgacccg gtcaccagat ccatcgcgcc ccccataccg ggcaccattt
    19861 tcccaggcac tacccagttc gcgaggtttg cttcttcgtc tacttgcaaa ccgccgagca
    19921 cgcaggcatc aatatgaccg ccacggatta gcgcaaatga catggcgcta tcaaacatgg
    19981 ctgcaccggg taaaatgccg cacggttgtc cgccagcgtt caccagatcg ggatgcgctg
    20041 ttgtgaccgg gcctaaaccg aggaagccgt tttccgattg cagagtgata tgaataccct
    20101 ctggtaaata attggcgacc attgtgggta aaccgatccc taagttaacg atgtcaccat
    20161 cacgaagctc ttgcgccaca cggcgcgcaa tacgttgttt cgcatccatt atttgctctc
    20221 ctgtgaaacg atgatgtggt cgataacggc accaggggtg acaatatggt caggttgcag
    20281 ctcgccggtt tcgaccagtt catccggctc taccagcgtg atatcagccg caagggctat
    20341 cagggggtta aagttgcggg cgctaaggtg ataggtcagg ttgccaagtg tgtcgcaacg
    20401 atgagcgcga attagcgcca ggtcggcgcg cagcgggcgt tcgagcagcc aggttttacc
    20461 gtcgagtgtc agtgtctgtt tgccttcctc tacgacggtg ccgacaccag ttggggtgag
    20521 aaaaccacca agtccagctc cgccacagcg aatttgctcg attagcgtac cttgcggcac
    20581 cagaacgacg tccatctcac cagatatcat gcgccgacct gtttccgggt tggtgccgat
    20641 atgtgaagca atcactttgc ggactcgacc attgacgatg agcggaccga tgccggtatc
    20701 aacaaacgcg gtatcattgg ctatcaatgt caggtcgcga acaccagatt ccagtaatgc
    20761 ttcaaccagg cgggatggag tgccaatccc cataaatccg cccaccatga tggtcatgcc
    20821 gtcacgaaag aagccggtgg cgtcttgtaa tgtcatcaat tttgttttca ttttttatcc
    20881 ctcttgcata caccgttgtg ggtacagtca gggaatagca aggggagtgc cagaatgaga
    20941 ttattgaatg ttgttattta actaattgaa tagaaaagga taaattagat ggggattcat
    21001 aaggagaatg agcggctatg cagaaaattg cgcactgtgc aaatttctgc atagcaagtt
    21061 ttggcgttat acatccgccg gatcgatacc gtattcctgg agtttataca tcaatgcacg
    21121 gcgactgatg cccagcatta aagcagtgcg ggttcggttt ccttcttgtt gttccagcac
    21181 ttccataatg atgcgttttt cgacgcgttt aatttcctct tttaaattac gctcaccgac
    21241 aggggctgtt tttgcttcac cagcattaca gactggctga cgaatctgcg gcggaagatc
    21301 ctcagaaaaa atgatcgggc ctgaattcat cacgacggcg cgttcaataa cgttggaaag
    21361 ctctcgaata tttcctggcc atgaccaggc ggtaagcagt gacattgcca tcggatcgat
    21421 gtcgataata tcgcgctgat tctcactact gaatttttgc aaaaagtgat tagctaacag
    21481 ggaaatatct tcccggcgat cgcgcagagg cggcagtatt aaatgaataa cgttaaggcg
    21541 ataaaagaga tcttcacgga aggtgccttc tttcaccatt gcctgcaagt cgcggttggt
    21601 ggcagcaatg atgcggatat caacttttat ggtctgatgg ccgccaatcc gttcaaattc
    21661 ccgttcctgc agaatgcgta gtaatttggc ttgcagtacc agcggcattt cgccaatttc
    21721 gtcgaggagc aaagtacctt cgttggcacg ttcaaataat ccctgacgca aggtttgtgc
    21781 accagtaaat gcaccttttt catgaccaaa cagttcactt tcgagcaacg attccggcag
    21841 cgccgcgcag ttgactttaa tgaacggccc ctttgcccgc cgcgaattgt agtgaatcgc
    21901 tctggcaatc aactctttcc cggtgccgct ttcaccgcta atcaagacgc tggcctgaga
    21961 aagggcaatt ttggcggtgt ctttgcagat gtccatcatc gccgggctgt tggtgagaat
    22021 gtgcccccat tgccagctgg tgctcagtgc ctggtgcaga tgacggatct cttttttcat
    22081 tgactggagt tgtaaagcgc gctgaacgat taaattcaac tcatcgagat caaacggttt
    22141 gataacatag tcgaaggctc cgcagcgtag tgcttcgacg gcggtttcca cttcagcata
    22201 ggccgtcatc agaataacgg gtgtccgggt ctcatggctg cgcatctcct ttagtgcctt
    22261 gatgccgtcc atctctggca tgcggatatc catcaacacc acgtcagggt gaatatcggc
    22321 aaacaggtgt aatgctgtgc gcccgttgtt cgcacaatgt gtttcgaatc cttgtagtgc
    22381 aaaagcggtg ctcagcatac ggcgaacatt atcttcatca tccacaataa ggatgcgatt
    22441 aatagcagtc atacagtctg atttccctgc gggttgatcg gtaaaataag cgtgaaggtt
    22501 gcgccgtagc ccggcaaact ggcgacgcga atatcaccct gatgggcatt aatgatgcgt
    22561 tgacttaacg ccagaccaag cccggttcct gaggctttgg tagtgaaaaa gggatcgaag
    22621 atcttttttt gcagcgagag atcaatgcca ctgccgttgt cctctatcga aatggcctgt
    22681 tgcgagtcgc tatactgcca ggttcgaatg cgaattttcc ctcgtgcact gatagcctgg
    22741 acggcattga tcaggatatt cagtagtacc tgtttgagca gttcacgatc ggcgttaatc
    22801 gggcttaatt cattatccag tttgcttatg aagtcgaccc gcgcttgtac gccggcggtt
    22861 tgtaccagta ccagagtttc ttcaaccaat gcattgaggc tgacttgttg ccattgactg
    22921 tggcgtggac gtgaaaattc gagcaattgc tgaataactt tgttaattga atcgatttct
    22981 ttgagtacta cggacagata ttcctgatgt attgggtcac gggtttgttg gtgcaagatc
    23041 tgtacataac cacgaatagc tgttagcgga ttacgtactt catgcgccac gccagccatc
    23101 agctcaccca gtgtggcgag gcgttctgct tgcgccatgc ggcgctgggt ttctttgcgg
    23161 gcagttaaat cagagaaaat caccaaagca cctatcattt caccgtgcgt gttatgaata
    23221 cgactggtcg tgacactgag ttcaatggtg cggtcacggc ctggaaaact gatctccagt
    23281 gctacatgtt cggtgccatg ttccagcgta tccagtactg gactgtagaa ctgagtattg
    23341 tcgaacaaca tggagtaagg ctgccctacc agttcatgac gttgatagcc agtgatgact
    23401 tctgctgctg ggttcatggt ggttacatca ccctggcggt caatggcaat gacgccatcg
    23461 gcagcgtttt caataatcag atcgttaagt gttcgcgttt cacgcagtgc ctgggcgagg
    23521 ttgttaacac tctgactgat ttgccccatt tcaccgggca attgtggtaa tcgagtggga
    23581 atattttgtg ccagagtcga gaggccatcg gtgatgatat ctatattggc gctcaggcga
    23641 cgggagaaaa ggacaatcaa cagcaggctt atcagcaaac cggcggtgag cacaatgata
    23701 atcctcacat ccattttcca ggcctggcgg cgaatatctt cggttaattc attggcccag
    23761 atatagccga ggatttcacc attacgctca atgggaatca ttgaattcaa aatatcgccg
    23821 cgcacctgcc tgcctgaata aaccaaaggg gtatttgtac gcatgacttc gcgaccaggg
    23881 tgatctgcgg caatggtaac gcccacatta ttctgatata gcgctgaagg cgcgtaggtg
    23941 attatcgcat ccagcgtttt gttgtaataa ccagcgccga tgccagggaa ggcgtgagtg
    24001 atattttcgg taatgggggc aagttctgca tttaatgcgc ggatacgctc ctcacgcggt
    24061 aagtcgatgt agagatcgta gcgattgcct agcgcctgat taagcaggtt gaccacggca
    24121 gaaagttttt tctctttttc agataagact gctgaacgtc cttccgtttc tacgatataa
    24181 ccaatagtaa gcgttgggac aatgaccatc aggattgcca tcaggatcat ttgattgcgt
    24241 aagcggcgtg gataaatcca cttcatataa tgcatgctga aatccactag tcttgtccgg
    24301 tatatgacga ttatcagagg ttaaggtgat gatttctcgg cggtgtatca tattccagag
    24361 aagagagaac attgcggtaa cacgctttta ccgctacctt aaccacactc catcggtcac
    24421 ctgaggcgga gcttcgcccc tttgaaatac cttgcttctt ttcgtacaac cctgaaagcc
    24481 tcgcgctaca tgttcagagc attggcgtta gtgctctggc tgttgattgc tttttcatcc
    24541 gttttttaca tcgttaatgc gttacatcag cgagaatcgg aaattcgtca ggaatttaat
    24601 ctgagttccg atcaggctca gcgctttatt caacgcacct ctgatgtgat gaaagagctg
    24661 aagtacatag ctgaaaatcg cttatcggca gaaaacggtg tgctttcccc gcgtggacga
    24721 gaaacgcaga cggatgtgcc tgcgtttgaa ccgctatttg ctgactctga ttgttccgca
    24781 atgagtaaca cctggcgagg ttctctggag tcattggcgt ggtttatgcg ctactggcgc
    24841 gataattttt ctgcggctta cgatctaaac cgggtatttt taatcggcag cgataacctc
    24901 tgcatggcca atttcggtct gcgtgatatg ccagtggaac gcgataccgc gttgaaagcg
    24961 ttgcatgaac gcatcaataa atatcgaaat gcaccacaag atgatagcgg cagtaacctc
    25021 tactggatca gcgaaggtcc gcgccctggc gtcgggtatt tttacgcgtt gacgccagtt
    25081 tatctggcga accggttgca ggcgcttttg ggtgtcgagc aaaccatccg gatggagaac
    25141 tttttcttac ctggtacgtt gccgatgggg gttaccattc ttgatgaaaa tggtcatacc
    25201 ctgatttcgc ttaccggacc agaaagcaaa attaagggcg atcctcgctg gatgcaggaa
    25261 cgctcctggt ttggctatac ggaagggttc cgggaactgg tgctgaagaa aaatctgcca
    25321 ccctcatcgc taagcatcgt ttattcagtg ccagttgata aggtgctgga acgcattcgc
    25381 atgttgatcc ttaacgcaat tttgctgaat gtgcttgccg gagctgcatt gtttactctc
    25441 gcgcggatgt acgagcggcg tattttcatt ccggcggaaa gcgacgccct gcgactggaa
    25501 gaacatgagc aatttaatcg caagattgtc gcctccgcgc cagtgggtat ctgcattttg
    25561 cgtaccgctg atggcgtcaa tattttaagt aacgaactgg cgcataccta tctcaatatg
    25621 cttacgcatg aggaccgcca acggctgaca caaattatct gtgggcagca ggtcaatttt
    25681 gttgatgtcc tgaccagcaa caataccaat ctgcaaatca gcttcgtcca ttcgcgctat
    25741 cgtaatgaaa acgtggccat ttgtgtgctg gtggatgttt cttcgcgcgt gaagatggaa
    25801 gagtcgttgc aggagatggc acaagcagcg gaacaggcga gccagtcaaa atcgatgttc
    25861 ctggccaccg tcagtcatga gctgcgaacg ccgctgtatg gcattatcgg taacctggat
    25921 ctgttgcaaa ccaaagagtt accgaaaggc gtcgatcgtc tggtgacggc aatgaacaac
    25981 tcttccagcc tgttgttgaa aattatcagc gatattctcg atttctcgaa aattgaatca
    26041 gaacagttga agatcgaacc gcgtgagttt tcaccgcgtg aagtgatgaa ccacatcacc
    26101 gccaactatt taccgctggt ggtacgcaag cagttaggct tgtactgctt tattgaaccg
    26161 gatgtgccag tggccttaaa tggcgacccg atgcgtttac agcaggtcat ctccaacctg
    26221 ttgagtaacg ccataaaatt caccgatacc ggctgtatag ttttgcatgt tcgcgctgat
    26281 ggcgattatc tctctatccg tgttcgcgat accggcgtgg ggattccggc gaaagaagtc
    26341 gtgcgcttgt ttgatccctt cttccaggtc ggaacgggcg tacagcgaaa cttccagggg
    26401 accggtctgg gtctggcgat ttgtgaaaaa ctgatcagca tgatggacgg cgacatctcg
    26461 gtagattcag aaccgggaat gggcagccag tttaccgtgc gtattccgtt gtacggcgct
    26521 cagtatccgc agaaaaaagg cgtggaaggg ttgagtggta aacgctgctg gctggcggtc
    26581 cgcaatgcgt cgctctgtca gttcctggaa accagtttgc agcgcagcgg catcgtcgtt
    26641 acaacatacg aagggcagga accgactccc gaagatgtgt taatcactga cgaggtagtg
    26701 agtaaaaaat ggcagggcag agcggtagtg accttctgtc gtcgccatat tggtattccg
    26761 ctggagaaag cgccagggga gtgggtacac agtgtggctg ctccgcatga gctaccggca
    26821 ttgttggcgc gtatttattt gatcgagatg gagagcgacg atcctgctaa cgctctgccg
    26881 tcgacggaca aagcggtcag cgataatgac gatatgatga ttctggtcgt ggatgatcat
    26941 ccgattaacc ggcgtctgct ggcagatcag ttgggatcgt tgggctatca atgtaaaacc
    27001 gcgaatgatg gcgtcgatgc gcttaatgta cttaacaaga atcatattga tatcgtgctt
    27061 agcgacgtca atatgccaaa tatggatggt taccgcttga cgcaacgcat tcgtcagttg
    27121 ggactgacgt tgccggtaat cggagtaact gctaatgcgt tggctgaaga gaagcagcgg
    27181 tgtctggagt ccggtatgga cagttgcctg tcgaagccgg taacgctgga tgtgataaaa
    27241 cagacgctga cggtatatgc cgagagggtc aggaaatcgc gggaatcgta ggacggataa
    27301 ggcgtttacg ccgcatctgg cactcagtgc aaatgccaga tgcgacgctg acgcgtctta
    27361 tctggcctac tttaatgtaa tctgttgaaa taatgggaat cgtaggacgg ataaggcgtt
    27421 tacgccgcat ccggcaatcc gtgcaaatgc cagataagac actaacgcgt cttatctggc
    27481 ctacaggtga ttagtcttta tctgccggac ttaacgtcac tgaagagaga taattcagca
    27541 gggcaatatc gttctcgaca cccagcttca tcatcgcaga tttcttctgg ctactgatgg
    27601 ttttaatact gcggttcagc tttttagcga tctcggtcac caggaagcct tccgcaaaca
    27661 ggcgcagaac ttcactctct tttggcgaga gacgcttgtc accgtaacca ccagcactga
    27721 ttttttccaa caggcgagaa acgctttccg gggtaaattt tttccctttc tgcagcgcgg
    27781 cgagagcttt cggcagatcg gtcggtgcac cttgtttcag cacgatccct tcgatatcca
    27841 gatccaatac cgcactaaga atcgccgggt tgttgttcat ggtcagaaca atgatcgaca
    27901 ggcttgggaa atggcgcttg atgtacttga ttaaggtaat gccatcgccg tacttatcgc
    27961 caggcatgga gagatcggta atcaacacat gcgcatccag tttcggcagg ttgttgatca
    28021 gtgctgtaga gtcttcaaat tcgccgacaa cattcaccca ctcaatttgc tcaagtgatt
    28081 tgcgaatacc gaacaagact atcggatggt catcggcaat aattacgttc atattgttca
    28141 tgtaataggc taccttgcta cagcaagctc ttgacataac tgtcaatgtc gctgatgtat
    28201 ttttctattc ctggaacatc cttctcacga atcagatgtt ccagcgtttc acataactgc
    28261 ttgccgggta ccagatttag catggcaaat acgcctttaa gacgatgagc cgtttgggct
    28321 aacgcagcaa agtcactggt tgctgcttca gtatatagcc tcttaacatc atccggtact
    28381 gtgtctacaa agagcgcata atagccgctg gcatggagtt gcgcattttc atctccgccc
    28441 agaggcgatt ctgtcacctc ttcctgcgcc agttgcactt caattaattg taagaccgct
    28501 tcctgcatag cgttgctcat attgaaattg acgcacaatt gaccaggccc aatttcccgt
    28561 acgccagact catcatcgct taaaagcaag ccagaggcag taagattaga cggattatcc
    28621 gttaaaaaga tatcataatc ttgactaatt aatctttcat cgggtgtgat acaggttgca
    28681 ccccaatttt ctaactggcg agtgacaata ttccgaattt ctgccgaagt aacatccacc
    28741 attacgcaga catcatccag taatcgctct tcttcctctt caacttccgg gtcagctgcg
    28801 agcattttga tatgcacaga gtagcgtgta ccaagcccat cccgcgtttt gatgtttaaa
    28861 tgaccgccca gtttacgcgc cagttgatcg ctcagccaga atgccagcgg gtccgccttg
    28921 ccatagcgat cattttgggt ctggttgata aacgggaagt gcaaattatc catttcatga
    28981 atgcttacgc cttctccagt gtccagaatg cggaacgtca ggcggtcttc ggaggactca
    29041 tcctgatcaa cctcaagggt gatttttccc aattgcgttg aggtcacggc atattgcatc
    29101 agtagtagca aaatacgtcg taaggcatcg cgatcgccgc ggcgcatatc gtgtgctttc
    29161 agatgattgt taatcagcag ttgcagacct ttacgcttga tggcaggcaa cactgaaggc
    29221 actacttcat caattaaatc ctgcacggag aacagctccg tctcactttt ccagctatcg
    29281 tccgcaagca tgttcgctaa ctgtatttca tcgaccagcc gcaccagcac atctgcctga
    29341 ttcgccagtt gtttgccttc cggggcgttg agtttagccg cgctctccgc cagggactgt
    29401 gcgggttctt tcagcgcatc gccaatgttt ttcataaagg tcatccgccc ctgctggttt
    29461 ttctcataca gacgctgcgc ctgcttgagt ttcttgttta ccagcacttc gcgatcctga
    29521 tcgcgaataa tgaaaatttg tgtgcgcggc gcaacctggc tgcggaacat gcggatctca
    29581 tacagctcgt tattgatcgt cgcctgaata atcccctgat gctgttccgc catggtggtg
    29641 atgttttgca gattcaaatg cggcagcaaa tgatcggcaa ttttgttact tatgactgtg
    29701 cggttcgatt cctgatcgtg aaccagcagg ccgagcggca gcagtgagac tatctcttca
    29761 ttaattgccc gtaaaatgcg caattcgtta ttgaccgccg tgctggggac gctttctgta
    29821 ctgcggctgg agaaatggcg gaatgtggta tagccaaata acgccagcgc cagcaaaccg
    29881 atgttcagca gcagtggcag cagaatgttt tgcaacgtat ccagcagtaa ggtgccgtaa
    29941 ggaacctgcc agaccaggcg catatcggta gagttgagtg ccgaggagat ttcaatcttc
    30001 gtactgttaa agtggatact gacgctatcc gtcccttctt tctcattttc attgtttccc
    30061 gttgccgtcg cgtctggctc aaggcggaaa ctgtccagcg gcatacccgg tgggatcaaa
    30121 tcattaatcg gcagatcaaa agccacgacc gttgccagat gtcccggctg gttgaacgtg
    30181 gtacgcaagg taaagtaatg accgttctgc caggccaggc ggcgcatgtt agaaaaactt
    30241 tcgcgttcat cgagggcgtt ggcctgttgc aacatctctg cacgacgtga atcaacaatg
    30301 tcgctgacgg tcgattcttt aaatccggag gtgagatctt tgaggggcag ggttgagatc
    30361 agcaccagac tgttatcctg accattcagg taatacatcg accacggtac attttctgcg
    30421 ccccacaatg tgtccagata ggtggacatc cgttgagtca tctcaagcgt tgagttgtcg
    30481 tgagagccaa agattaaggc ttccgttttg cggcgtggtt tctccagata gtagacatcc
    30541 tgtttcagtc gcgtctcttg taaaccttcg ccggaggagg gggaggtcgt cgcggcaatg
    30601 ttgtcgtaga tctgccaggt cacgtaacgc caggtatcga cgcgcttttg gattgcgtgg
    30661 gtaatgtcga caatctggta acttttatct ttcagccagg cgttaacggc gctttgtacc
    30721 attacaccca tcgtcaccag taacacaatg atcaacagta aaaagaagcg ggtaatgctc
    30781 cccggtagga gtgaaaagcg ggtcgtggcc gttgtctctt tctgacgcat tcgtgtttat
    30841 gacctgttaa aacttcgcga agtgataggg cagtataaag ggtacagtgt gaaattccag
    30901 actcttacct gattgatgat tctctattct ccagtgatat ttatcatcct gcattggggg
    30961 aaatagatgt acaagcgcta ttttgctgta caaataacct acaaaaagcc taacgaaatt
    31021 aattgcaaat aaacgaagat tgctggaaat tatgctgatg ttaattattt gtgaaatagt
    31081 taacaagcgt tatagttttt ctgtggtagc acagaataat gaaaagtgtg taaagaaggg
    31141 taaaaaaaac cggatgcgag gcatccggtt gaaatagggg taaacagaca ttcagaaatg
    31201 aatgacggta ataaataaag ttaatgatga tagcaggaga tattctagtt gcgagtgaag
    31261 gttttgtttt gacattcagt gctgtcaaat acttaagaat aagttattga ttttaacctt
    31321 gaattaatat tgcttgatgt taggtgctta tttggtcatt ccgcaataat cttaaaaagt
    31381 tcccttgtat ttacattttg aaacatctat ggcgataaat gaaacatctt aaaggtttta
    31441 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggacttg ccgactgatt
    31501 aatgagggtt aatcagtatg cagtggcata aaaaagcaaa taaaggcata taacagaggg
    31561 ttaataacat gaaagttaaa gtactgtccc tcctggtccc agctctgctg gtagcaggcg
    31621 cagcaaacgc tgctgaagtt tacaataaag acggcaacaa attagatctg tacggtaaag
    31681 tagacggcct gcactatttc tctgacaaca agtcagaaga tggcgaccag acctacatgc
    31741 gtcttggttt caaaggcgaa actcaggtta ctgaccaact gactggttac ggccagtggg
    31801 aatatcagat ccagggcaac gctccggaaa gcgaaaacaa ctcctggacc cgtgtggcat
    31861 ttgctggtct gaaattccag gacatcggtt ctttcgacta cggtcgtaac tacggcgttg
    31921 tttacgacgt aacttcctgg accgacgttc tgccagaatt cggtggcgac acctacggtt
    31981 ctgacaactt catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct
    32041 tcggtctggt tgacggcctg aactttgctg ttcagtacca gggccagaat ggcagcgtaa
    32101 gcggtgaaaa tgatccggat ttcactggtc atggcatcac caacaatggt cgtaaagctc
    32161 tgcgtcagaa cggcgacggt gtcggcggat ctatcactta tgattacgaa ggcttcggcg
    32221 ttggtgctgc agtttccagc tccaaacgta cctgggatca gaacaacact ggcctgatcg
    32281 gtactggcga ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct
    32341 acctggctgc tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg
    32401 cgaacaaagc acagaacttc gaagctgttg ctcagtacca gttcgacttt ggtctgcgtc
    32461 cgtctgtagc atacctgcag tctaaaggta aaaacctggg tgtcgttgct ggtcgtaact
    32521 acgacgacga agatatcctg aaatatgttg atgttggtgc tacctactac ttcaacaaaa
    32581 acatgtccac ctacgttgac tacaaaatca acctgctgga cgacaaccag ttcacccgcg
    32641 ctgctggcat caacactgat gacatcgtag ctctgggtct ggtttaccag ttctaatctc
    32701 gatttatatc gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaacac
    32761 agttttttgg tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct
    32821 ttacccgcgt ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac
    32881 aacccgccaa aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct
    32941 tctggcgtgc cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga
    33001 ttcagaccca gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc
    33061 tgatgcgctt taacgactcg caaagtttgt cgccgtggcc ggtaagtgaa gcgatggccg
    33121 atatcgtcac cacctcgctg cgcattggcg caaggaccga tggtgcgatg gatataaccg
    33181 tcgggccgct ggtgaatctg tggggctttg gcccggaaca acagccggtt caaattccga
    33241 gccaggaaca gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatta
    33301 atcagtcgca tcagcaatat ctgcaaaaaa acctgccgga tttatatgtc gatctctcca
    33361 ctgtcggcga aggctatgcg gcggatcatc tggcacgctt gatggagcag gaagggattt
    33421 cccgctatct ggtgtcggtg ggcggcgcgc tgaacagccg tggcatgaac ggtgaaggcc
    33481 agccgtggcg ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggccgtgg
    33541 tggatatcaa cggtcatggc attagcacct ctggcagcta ccgtaactat tacgaactgg
    33601 acggcaaacg cctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc
    33661 tggtatccgt gacggtgatt gccccgacgg cgctggaagc cgacgcctgg gatactggct
    33721 taatggtgct cgggccagag aaagccaaag aagtcgttcg ccgggaaggg ctggcggttt
    33781 atatgatcac caaagagggc gatagcttta aaacctggat gtcgccgcag tttaaaagct
    33841 tccttatcag cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg
    33901 gcagactaaa ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact
    33961 gacgatcaac gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt
    34021 ttcgccgtgc gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgctttg
    34081 cgggaaaacg tttctttcta tgccgatgcc agtgaggcgc ttgccgctgg ctttcgcccc
    34141 tgcaaacgtt gtcagccaga caaagccaat ccccaacaac atcgccttga taaaatcacc
    34201 cacgcctgcc gactgctgga acaggaaacg cctgtaacgc tggaatcctt agccgaacag
    34261 ctggcgatga gtccgttcca tttgcataga ttgtttaaag cgactaccgg aatgacgccg
    34321 aaagcctggc agcaggcctg gcgtgctcgc cgattgcggg agtctctggc aaaaggggag
    34381 agcgtgactc aggcgattct cagtgccgga ttccccgacg gcagcagtta ctaccgtaaa
    34441 gccgatgaaa cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg
    34501 gtgcgttacg cgctggctga ttgtgagctg ggccgttgcc tggtggcaga aagcgagcgg
    34561 gggatttgcg cgatattgtt gggcgatgat gatgcgacat taatcagcga gttgcagcag
    34621 atgtttcccg ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa
    34681 gtgatcgcca gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc
    34741 actgcttttc agcaacaagt ctggcaggca ctgcgcacga taccttgtgg tgaaaccgtc
    34801 agttatcagc aactggcgaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc
    34861 gcctgtgccg ccaacaagct cgctatcgtt ataccttgtc atcgggtggt ccgtggtgat
    34921 ggctcacttt ccggttaccg ctggggcgtg tcgcgcaaag cgcaactgct gcgccgcgaa
    34981 gttgaaaatg aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagaac
    35041 cactggcggc tggagcggtg attttgcacc gttttgcttt taacgctgcg gagcaactga
    35101 tccgcgatat taatgacgtt gccagccagt cgccgtttcg ccagatggtc actccaggcg
    35161 gatataccat gtcggtggcg atgaccaact gcgggcgtct gggctggacc acccatcggc
    35221 agggttatct ctatttgccc attgatccgc aaacaaataa accgtggccc gccatgccgc
    35281 agagttttca taatttatgt caacgtgcgg ctacggcggc gggctatcca gatttccagc
    35341 cagatgcttg tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata
    35401 aagacgaacc ggatctgcgc gcgccgattg tttctgtttc tctgggctta cccgcgattt
    35461 ttcaatttgg cggcctgaaa cgaaatgatc cgctaaaaag attgttgctg gaacatggcg
    35521 atgtggtggt atggggcggt gaatcgcggc tgttttatca cggtattcaa ccgttgaaag
    35581 cggggtttca tccactcacc accgactgcc gctacaacct gacattccgt caggcaggta
    35641 aaaaagaata aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc
    35701 tgtaattcat tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc
    35761 gctggccatt tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct
    35821 taattgcttt tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtgttgc
    35881 cggagtttct gggattattg ttgctgttga tggcagtcac tctcggatcg caactggcgc
    35941 tcaccacttt ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc
    36001 tggataccca cgtcgagcgc attgaacaac ttggtagcgg ttcgttgctg gcggggttaa
    36061 ccagcgatgt gcgcaatatc accattgctt ttgtgcgtct gccggaactg gtgcagggga
    36121 tcattctcac tatcggttca gcggcgtatt tgtggatgct gtcgggcaaa atgttgctgg
    36181 taacggctat ctggatggca gtcaccatct ggggtggttt tatactggtg gcgcgggtgt
    36241 acaaacatat ggcgaccctg cgtgaaaccg aagataagct gtacacggat tttcaaaccg
    36301 tactcgaagg gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca
    36361 acctctacat tcctgatgcg caagagtatc gccatcatat tatccgcgca gacaccttcc
    36421 atcttagtgc cgtgaactgg tcaaacatca tgatgctggg cgctatcggc ctggtgttct
    36481 ggatggcgaa cagtctcggt tgggctgata ccaacgttgc cgcgacctat tcgttgacgc
    36541 tgttattcct gcgtacgccg ctgctttcgg cggttggcgc attaccgacg ctgctgacgg
    36601 cgcaggtggc gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gcagagtttc
    36661 cgcgcccgca ggcttttccc aactggcaaa cgctggagct gcgtaacgtg acgtttgctt
    36721 atcaggataa cgcgttttcc gttgggccga ttaacctcac catcaaacgt ggtgaactgc
    36781 tgtttctgat tggtggcaac ggtagtggaa aatcgacgct ggcgatgttg ctgacgggct
    36841 tgtaccaacc acaaagcggc gaaatcttgc tggatggcaa acctgtcagc ggcgaacaac
    36901 cggaagatta tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac
    36961 tgctggggcc ggaggggcaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc
    37021 tgaaaatggc tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa
    37081 aagggcagaa aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc
    37141 tgctggatga atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgc
    37201 tgctgccgct gatgcaggag atgggtaaaa ctattttcgc catcagtcat gatgatcatt
    37261 actttatcca cgccgaccgc ctgctggaaa tgcgcaacgg gcaacttagc gagctgacag
    37321 gcgaagagcg cgatgctgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg
    37381 ctgacctggc ggactgctgc cggcaggtca atattctaaa aataccttcg tttctaataa
    37441 ccctataatt aatcaacgaa attataatgt ttctaaaatt agaa
//
LOCUS       DAZGJT010000038        37265 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZGJT010000038 DAZGJT010000000
VERSION     DAZGJT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.038"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..687
                     /gene="aslA"
                     /locus_tag="ACHKFK_002088"
     CDS             <1..687
                     /gene="aslA"
                     /locus_tag="ACHKFK_002088"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO9052431.1"
                     /translation="DCMVEMNDIFANLYKALEKNGQLDNTLIVFTSDNGPEAEVPPHG
                     RTPFRGAKGSTWEGGVRVPTFVYWKGMIQPRKSDGIVDLADLFPTALDLAGHPGAKVA
                     NLVPKTTFIDGVDQTSFFLGTNGQSNRKAEHYFLNGKLSAVRMDEFKYHVLIQQPYAY
                     TQSGYQGGFTGTVMQTAGSSVFNLYTDPQESDSIGVRHIPMGVPLQTEMHAYMEILKK
                     YPPRAQIKSD"
     gene            complement(778..2013)
                     /gene="aslB"
                     /locus_tag="ACHKFK_002089"
     CDS             complement(778..2013)
                     /gene="aslB"
                     /locus_tag="ACHKFK_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9052432.1"
                     /translation="MQQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYHEKPVTH
                     MDDDTLEAYVRHYIAASETQNEVAFTWQGGEPTLLGLDFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSALQPLQVYDFLCDAGVEFIQFIPVVERLADETAAHAGLKLH
                     APGDIQGELTEWSVCPQEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMLQQTIAEMIDSPQQQAFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMVDLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(2160..2236)
                     /locus_tag="ACHKFK_002090"
     tRNA            complement(2160..2236)
                     /locus_tag="ACHKFK_002090"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2200..2202),aa:Pro,seq:tgg)
     gene            complement(2279..2365)
                     /locus_tag="ACHKFK_002091"
     tRNA            complement(2279..2365)
                     /locus_tag="ACHKFK_002091"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2329..2331),aa:Leu,seq:cag)
     gene            complement(2386..2461)
                     /locus_tag="ACHKFK_002092"
     tRNA            complement(2386..2461)
                     /locus_tag="ACHKFK_002092"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2426..2428),aa:His,seq:gtg)
     gene            complement(2520..2596)
                     /locus_tag="ACHKFK_002093"
     tRNA            complement(2520..2596)
                     /locus_tag="ACHKFK_002093"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2560..2562),aa:Arg,seq:ccg)
     gene            complement(2699..4084)
                     /gene="yifK"
                     /locus_tag="ACHKFK_002094"
     CDS             complement(2699..4084)
                     /gene="yifK"
                     /locus_tag="ACHKFK_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9052433.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLVVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(4275..5015)
                     /gene="wecG"
                     /locus_tag="ACHKFK_002095"
     CDS             complement(4275..5015)
                     /gene="wecG"
                     /locus_tag="ACHKFK_002095"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO9052434.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTQVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(5018..6370)
                     /gene="wzyE"
                     /locus_tag="ACHKFK_002096"
     CDS             complement(5018..6370)
                     /gene="wzyE"
                     /locus_tag="ACHKFK_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO9052435.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADAPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(6367..7446)
                     /gene="rffT"
                     /locus_tag="ACHKFK_002097"
                     /gene_synonym="wecF"
     CDS             complement(6367..7446)
                     /gene="rffT"
                     /locus_tag="ACHKFK_002097"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO9052436.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSGHAREFMVAGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPKLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQVLSEKLEFDAYLTLLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7443..8693)
                     /gene="wzxE"
                     /locus_tag="ACHKFK_002098"
     CDS             complement(7443..8693)
                     /gene="wzxE"
                     /locus_tag="ACHKFK_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO9052437.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAVIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDIIREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8695..9825)
                     /gene="rffA"
                     /locus_tag="ACHKFK_002099"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8695..9825)
                     /gene="rffA"
                     /locus_tag="ACHKFK_002099"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO9052438.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAAERINQQRLALWQNYYDALAPLAEAGR
                     IELPSIPDDCVQNAHMFYIKLRDIDDRSALISFLKEAEIMAVFHYIPLHASPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9830..10504)
                     /gene="rffC"
                     /locus_tag="ACHKFK_002100"
                     /gene_synonym="wecD"
     CDS             complement(9830..10504)
                     /gene="rffC"
                     /locus_tag="ACHKFK_002100"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO9052439.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10482..11363)
                     /gene="rfbA"
                     /locus_tag="ACHKFK_002101"
     CDS             complement(10482..11363)
                     /gene="rfbA"
                     /locus_tag="ACHKFK_002101"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9052440.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(11382..12449)
                     /gene="rffG"
                     /locus_tag="ACHKFK_002102"
     CDS             complement(11382..12449)
                     /gene="rffG"
                     /locus_tag="ACHKFK_002102"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9052441.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEYQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWTALTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     EIWWKQVQDGSYQGERLGLKG"
     gene            complement(12449..13711)
                     /gene="wecC"
                     /locus_tag="ACHKFK_002103"
     CDS             complement(12449..13711)
                     /gene="wecC"
                     /locus_tag="ACHKFK_002103"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO9052442.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGELADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAAADKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHELPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13708..14838)
                     /gene="wecB"
                     /locus_tag="ACHKFK_002104"
     CDS             complement(13708..14838)
                     /gene="wecB"
                     /locus_tag="ACHKFK_002104"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO9052443.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLLVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14894..15940)
                     /gene="wzzE"
                     /locus_tag="ACHKFK_002105"
     CDS             complement(14894..15940)
                     /gene="wzzE"
                     /locus_tag="ACHKFK_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO9052444.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIVFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15952..17055)
                     /gene="wecA"
                     /locus_tag="ACHKFK_002106"
     CDS             complement(15952..17055)
                     /gene="wecA"
                     /locus_tag="ACHKFK_002106"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO9052445.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(17295..18554)
                     /gene="rho"
                     /locus_tag="ACHKFK_002107"
     CDS             complement(17295..18554)
                     /gene="rho"
                     /locus_tag="ACHKFK_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO9052446.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18639..18740)
                     /gene="rhoL"
                     /locus_tag="ACHKFK_002108"
     CDS             complement(18639..18740)
                     /gene="rhoL"
                     /locus_tag="ACHKFK_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO9052447.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18881..19210)
                     /gene="trxA"
                     /locus_tag="ACHKFK_002109"
     CDS             complement(18881..19210)
                     /gene="trxA"
                     /locus_tag="ACHKFK_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO9052448.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            19341..20606
                     /gene="rhlB"
                     /locus_tag="ACHKFK_002110"
     CDS             19341..20606
                     /gene="rhlB"
                     /locus_tag="ACHKFK_002110"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO9052449.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20742..22226
                     /gene="gppA"
                     /locus_tag="ACHKFK_002111"
     CDS             20742..22226
                     /gene="gppA"
                     /locus_tag="ACHKFK_002111"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO9052450.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLTVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFYDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIDQESQWQSYVHWPLEVH"
     gene            complement(22273..24294)
                     /gene="rep"
                     /locus_tag="ACHKFK_002112"
     CDS             complement(22273..24294)
                     /gene="rep"
                     /locus_tag="ACHKFK_002112"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO9052451.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPTLKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24381..24662
                     /gene="ppiC"
                     /locus_tag="ACHKFK_002113"
     CDS             24381..24662
                     /gene="ppiC"
                     /locus_tag="ACHKFK_002113"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO9052452.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24708..26183)
                     /gene="ilvC"
                     /locus_tag="ACHKFK_002114"
     CDS             complement(24708..26183)
                     /gene="ilvC"
                     /locus_tag="ACHKFK_002114"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO9052453.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26333..27223
                     /gene="ilvY"
                     /locus_tag="ACHKFK_002115"
     CDS             26333..27223
                     /gene="ilvY"
                     /locus_tag="ACHKFK_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO9052454.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEDDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSADKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCV
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(27220..28764)
                     /gene="ilvA"
                     /locus_tag="ACHKFK_002116"
     CDS             complement(27220..28764)
                     /gene="ilvA"
                     /locus_tag="ACHKFK_002116"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO9052455.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28767..30617)
                     /gene="ilvD"
                     /locus_tag="ACHKFK_002117"
     CDS             complement(28767..30617)
                     /gene="ilvD"
                     /locus_tag="ACHKFK_002117"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO9052456.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRSNGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGNKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(30682..31611)
                     /gene="ilvE"
                     /locus_tag="ACHKFK_002118"
     CDS             complement(30682..31611)
                     /gene="ilvE"
                     /locus_tag="ACHKFK_002118"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO9052457.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31631..31894)
                     /gene="ilvM"
                     /locus_tag="ACHKFK_002119"
     CDS             complement(31631..31894)
                     /gene="ilvM"
                     /locus_tag="ACHKFK_002119"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO9052458.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31891..33537)
                     /gene="ilvG"
                     /locus_tag="ACHKFK_002120"
     CDS             complement(31891..33537)
                     /gene="ilvG"
                     /locus_tag="ACHKFK_002120"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO9052459.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDILGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVSALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(33540..33590)
                     /gene="ilvX"
                     /locus_tag="ACHKFK_002121"
     CDS             complement(33540..33590)
                     /gene="ilvX"
                     /locus_tag="ACHKFK_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO9052460.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33677..33775)
                     /gene="ilvL"
                     /locus_tag="ACHKFK_002122"
     CDS             complement(33677..33775)
                     /gene="ilvL"
                     /locus_tag="ACHKFK_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO9052461.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34128..35648
                     /gene="yifB"
                     /locus_tag="ACHKFK_002123"
     CDS             34128..35648
                     /gene="yifB"
                     /locus_tag="ACHKFK_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO9052462.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALEHPKPTNAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGENSTTVKQRVMAARERQFKRQNKLNAWLDNPEIRQFCKLESEDAQWLEET
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(35673..36011)
                     /gene="maoP"
                     /locus_tag="ACHKFK_002124"
     CDS             complement(35673..36011)
                     /gene="maoP"
                     /locus_tag="ACHKFK_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO9052463.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36130..36969
                     /gene="hdfR"
                     /locus_tag="ACHKFK_002125"
     CDS             36130..36969
                     /gene="hdfR"
                     /locus_tag="ACHKFK_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO9052464.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDVHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFCSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(37065..37140)
                     /locus_tag="ACHKFK_002126"
     tRNA            complement(37065..37140)
                     /locus_tag="ACHKFK_002126"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37105..37107),aa:Trp,seq:cca)
     gene            complement(37149..37225)
                     /locus_tag="ACHKFK_002127"
     tRNA            complement(37149..37225)
                     /locus_tag="ACHKFK_002127"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37189..37191),aa:Asp,seq:gtc)
BASE COUNT         9111 a        10205 c         9385 g         8564 t
ORIGIN      
        1 gactgcatgg tggagatgaa cgatatcttc gccaatctgt ataaagcact ggagaaaaac
       61 ggtcagcttg ataacacgct gattgtgttc acctctgata acggtccgga agccgaagtt
      121 ccgccgcacg gtcgcacgcc gttccgcggt gcgaaaggtt ctacatggga aggcggcgtt
      181 cgtgtgccaa ccttcgttta ctggaaaggc atgatccaac cgcgtaaatc tgacggcatt
      241 gtcgatctgg cggatctctt ccctacggca ctggatctgg cggggcatcc tggggcgaaa
      301 gtagcgaatt tagtaccgaa aaccaccttt atcgatggtg tggaccagac atctttcttc
      361 ctgggaacaa atggtcagtc taaccgtaag gccgagcact acttcctcaa cggtaaactt
      421 tctgctgtgc gtatggatga gttcaagtat cacgtcctga ttcagcaacc ttacgcttat
      481 acccagagcg gatatcaggg tggattcacc ggcacagtaa tgcaaacggc gggatcgtcg
      541 gtgtttaacc tgtacaccga tccgcaggaa agcgactcta tcggcgtgcg ccatatccca
      601 atgggtgtac cgctacagac cgaaatgcat gcgtatatgg agatcctgaa aaaatatcca
      661 ccacgcgcgc agattaaatc tgattaagcc ggcgctatcg ctgagatcta cctttgccgg
      721 atgcgatgct gacgcatccg acaatcaacc gcaggcggcc acaaaatggc tactttttta
      781 cttattcacc accagcaaat gcgcctgcat aatgtcgctg gccggacgac cgtgcgccag
      841 caaatcaacc attgctttaa gatatggcgg tagatggcgg aaataacgct gatacccggc
      901 acacaaataa ttcagcccgg gtttgccgct ggcatcgagc ataaagcggt gtttcgggca
      961 gcctccccag cacgctttta acacgttgca actgcgacac tgtgccggta actgcttgaa
     1021 tttatcttca ccaaatgcct gctgttgcgg ggaatcaatc atttctgcaa tcgtctgctg
     1081 aagcatattc cctagtcgat actgcggata aacatagtga tcgcaggcat aaacgtcacc
     1141 gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa ccgcgcccgg
     1201 tgcgccgaca aaattggcga acgcccattc gatattcatt acgaaaatct tgccgacgtc
     1261 gcgcttaatc cagtggtcga aaatcgccac cagaaactca ccgaactctt gcgggcacac
     1321 cgaccattcc gtcagttcgc cctgaatatc gccgggagca tgtagcttaa gtccagcatg
     1381 agcagccgtt tcatcagcca ggcgctcgac caccggaata aattggatga actcaacacc
     1441 tgcatcacac agaaaatcat agacttgcaa aggttgtaac gcactggtgc gattgacgca
     1501 gaccagcacg ttatagtcga catgatgttt ttgcaggagc gtcagagcac gcatcaccag
     1561 cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct cagccggacc
     1621 atccagcgat aaccctacaa gaaaatgatt ttctgccaga aatgcacacc attcgtcatc
     1681 gagcagcacg ccgttagtct ggaagctatt acttatcttc ctgccagcac catatttcgc
     1741 ctgtagcgcc acggcacggc ggtaaaaatc cagcccgagt aacgttggtt cgccgccctg
     1801 ccaggtaaaa gccacttcgt tttgggtttc gctggcagcg atatagtgac ggacatacgc
     1861 ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg tggtaaaggg attgtttttc
     1921 gagataaaaa cagtagtcac agttcagatt acaatcagaa ccgctcggtt tcgccatcac
     1981 atgaaaagca cgcgttggaa cctgttgctg catggtcgct ccttgcctga agtcatcctt
     2041 caataagtta agggcgaaat cataccgtgg cgcaatgcgt tattacgggc gtttattgcg
     2101 aagggaaagt gacgaaatgc acagaaaaca aaaaacccca ccgaagcggg gttcaaaatt
     2161 ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt gcgctaccaa
     2221 gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt taaagtcgtg
     2281 gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct gaagctagcg
     2341 cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg tggctaatgg
     2401 gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga gctatagcca
     2461 ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc ggtactaaat
     2521 ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc gctctatcca
     2581 gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt cccacccctc
     2641 tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt tttgctgctt
     2701 attcctccag tttatgcgct ttaccgtggc gattaagccc aaaaacttta taaatcgccg
     2761 tcaccaccag cataaagatg atgccgacaa acagcgacat acgcgtatct tcattaaagt
     2821 acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc caagggaaca
     2881 ggattgagcg gaacggatgg ctggcaattg ctgctttatg cgcacgacga aaacgcagct
     2941 ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca ctgtagacgt
     3001 aaacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca attagcagaa
     3061 tagcaataga tactgccaca cccgcaaccg gtacgccgtg acgggaaact ttcgccattg
     3121 ccgccggtaa ctgacggttt ttcgccagtg catagagcat acgtccgcaa ctgtacatgc
     3181 cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg cccgccgctg
     3241 cggtaatgcc gattttggcg aaagtcagta cgaacgggct accgttgctg cctatttcat
     3301 tccacgggaa gatagtaacg ataacgaaaa tcgcgccaac gtagaaaatc aggattcgcc
     3361 acagcacctt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct tcaccggcag
     3421 taatgccaat cagctccacg ccctggtagg acgccaccac aatacacaat gcggtcagga
     3481 accctttcca gccaccagca aagaaaccgc catgctctgt gagattgcta aaaccaatcg
     3541 actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca atcatcacga
     3601 taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac aacctcaccg
     3661 cagccagatt cgccaacgcc accagcgcca ccgcgatcaa tgcgggtatc cactgcgcca
     3721 tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag atccccaccg
     3781 ccatccacat aaaccagtaa gaccaggcgg tgagataacc aaagaatggg ctcatataac
     3841 gatgcgcgta aacggcgaac gaaccggtaa ccggttcgag gaacaacatt tcgcccattg
     3901 aacgcatgat gaaaaagacg aacagcccgg cgatgatata ggccaacaat acggatggcc
     3961 cggcccattt cagggtactg gcggccccca taaacaggcc gacgccaatg gtgcccccca
     4021 aggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt ttgttatctg
     4081 ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc gttatgggta
     4141 catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag cattaaaaat
     4201 ttgaatgctt tgtgtaataa aaaagcagac aggcgacgaa gtgaccactt cgtcgcttta
     4261 caaagagagg aaaatcagag gttgccggta tagtgccagc gtaaataacg cagcaaacga
     4321 agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc cagccccagc
     4381 gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta ggtcccgcca
     4441 acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa gatctcctgc
     4501 tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat gcgttcaaac
     4561 agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat attcacattc
     4621 cactggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc gcccacaaga
     4681 aatacctgtg tcccttcttt gcctgcgcgc gccatcagct cttcccagag atcggcccca
     4741 gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac aacgcttatg
     4801 ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcagc gttatcttca
     4861 atggtcagca ttttttcggc attaatggca accagcgttc cctgcttaag ctgcccgtca
     4921 gcgaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg taagccacgc
     4981 agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc tgcgtccgga
     5041 gcgatgattt tgttcgttta tgaatgagtc cggcgctttc aaaaagccag tacaacagtt
     5101 ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gaccacgcgt gagacaaacg
     5161 aatccagccc ttcacgcgcc agcacgatca tattgaagat cgccccgaag cagaaactgt
     5221 gcaatatcgc agccttatag cgattagtct cgcggttgcc cagctcatac agccagtcga
     5281 accatttgat aatcagtcca accacaatcg ccccgagcgg gatgaacaac gcgccgccca
     5341 taacaaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg ttattcagca
     5401 cttcccaggt aaagtagttg gcggagttca gcaccatact cgggcgaccc ggccacagcc
     5461 aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg aagtcgatgt
     5521 tgtcgtagtt ctgcaacagc aacgccagat tctcccacgg cgagaaagtg tcgcgagtga
     5581 gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag cgttttagcg
     5641 ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc cacaacgaaa
     5701 tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg atattggcgc
     5761 gagtgccgcc gacaatcatg taagtcaaca agccaaaggc aaccgtgctg acgaggaaaa
     5821 acagccacgc tttgctatcc tggcgcagaa aataaaccac cagcatcgcc gggatgaaaa
     5881 agtaaaagaa gcgttttaat gccacgccgg agacttcgct ggaaaagatc tgactgtaag
     5941 aattaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg ctcaccagag
     6001 cgatgcccat caggatcacc cacgtgagat tggtctccac gcggttcatg gtaaacagcg
     6061 gacggcgcgg tgcatcagca acgcgtttgc gtagacgggt tttgtaggtg acatagtaaa
     6121 ccgcgtagaa gcagcctgca gaaagcaacg cctgcaacaa gatttctggc ggcgcgacac
     6181 caacatcaaa gcgaaatacc agcacgctgg tcagcggaaa gccgaagaaa aaggtgagca
     6241 aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac tcaaaccagg
     6301 tcagcgtggc aataaacagc gtgcagagca gccagacaac aaacaggcca ctgaattgca
     6361 gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc cttgtagata
     6421 gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc gctgcgcttc
     6481 acgcacaata tcctcgttga gatcatcggt agtaaacagt actggcaaat gctgttccgt
     6541 catatcctgc cagaacggat tttcccggtt aagcacgcaa ggaatgcccg cctgaatcag
     6601 taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccga gatcgcactg
     6661 gcgaagtagc gtcagatagg cgtcaaattc cagtttttcg ctcagaactt gtaggttttc
     6721 ttcgctgaat aactccagtc ccgcctgacg aacttcctcg atgtacgctt cgttattagg
     6781 cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat gaacggcgcg
     6841 caaggcagca atatgctcat tgctgcggtc accggagttc cccaccagaa tggtcatttt
     6901 tccttcacgt tgccgatcgt tcgccatcgt attaagagaa gggtccatcc gcgtcgggaa
     6961 gtacagcagt tctccccgca cctttggatg cgttttggca aaaaagctca aatcaccgcg
     7021 ggtggcaaat acacagccga ctctcttttg cgccaggcga cgtagcgggt aaaaaagctt
     7081 atatctcaag ccactggaaa gctcatacag gtctgctccc cagatatgcc agaaaaactg
     7141 gctgggctta atgccaccac tcagcagagc cagccacagt ttgggattga actgaccgtg
     7201 gaaaaaaaat cgctgctgac ggttagcttt tgctttcgcg atgaccgctt ccgccagcga
     7261 ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta agccgtcgtc
     7321 cttgccagca accataaact cgcgcgcgtg cccgctcgtc gcggccagcg cgtcgttgaa
     7381 aaaccgcaga acggttcggt tatggtgagg gatatccgat cccagtacgt gaatcagtac
     7441 agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa atagacgata
     7501 taggttgcca tataggcctg tgctgcgccc agtgcgccat gcgcagggat tagccagtgg
     7561 gcaaatacca tcaataaagt gaactggctg atttccgcca gaatataaaa ccgcagtgac
     7621 gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac atcacccacc
     7681 aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag cagccagata
     7741 gcaaaatcac gcagcaacca gacggtaaaa ctcgccgccg ccactgccgg taagacgaat
     7801 ttcagcgatt taaccacttc ccggatgata tcgcgctttt ccgttagccg cgacaacgtg
     7861 ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc ggaaatactg
     7921 ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgccag cagtttacgc
     7981 atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag cgtaaatttg
     8041 ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa cgggatgaca
     8101 ccacgtttga tcaacatgat aacagcagga attaccacca gcgcgggaat cagcgccaga
     8161 cccagcaacg ccccttcata accgcccaaa cgataactga cgtagtacgc gagaacgcca
     8221 atcaagctac cgacaatcag agataacgca ttacctgcgg catcgcgaaa gcctttcatc
     8281 aacgccagta acaggttgcc ccaggcaatc cccatttgca ccagcgccac taaacgcacc
     8341 agcccctgat agtcggtatt accaaacaac ccctggctga ttggcgcagc tgccagcaca
     8401 aaaaccagcg ccattagcgt agagaagcca agtaccatcg ctgatgaagt gccgaccacg
     8461 cggcgcagct gttggggatt atcatgatac tgggcaacgt atttggttac gccgttaaag
     8521 atgcctgccc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt tgccgccagc
     8581 ccaagccccg ccggaccaaa tgacactgcc agcaacttac cgaccagtaa cccggcacca
     8641 atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga catatcagga
     8701 gaagtagttc aacaaggtcg caattaccgt acgctgattg acgggcgaca ggttgtagaa
     8761 caacggcagg cgcagcaagc gctcgctctc tttggtggtg tagcgatctt cgccgtggaa
     8821 ctcaccaaag cgttcccccg cagggcttgc gtgcagcgga atgtaatgga acaccgccat
     8881 gatttccgct tccttcagga agctaatcaa cgcgctccgg tcatcaatat cccgcagctt
     8941 gatgtagaac atatgcgcgt tctgcacgca gtcatccgga atcgacggca gctcgatacg
     9001 cccggcttcc gccagaggtg ctaacgcatc gtagtagttt tgccacagcg ccagacgttg
     9061 ctggttgatg cgttcggctg cttccagttg cgcccacagg tacgcagctt gcagatcgga
     9121 catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac cacggaagaa
     9181 ctggctgcgg tttgtgcctt tttcacggat gatctcggct cgttcgatca acgctttatc
     9241 gttaatcagc gtcgcgccgc cttcaccacc cgccgtgtag tttttggttt catggaagct
     9301 aaagcagcca atatgaccaa tggttcccag cgcacgccct ttgtaagtgg acatcacgcc
     9361 ctgagcggca tcttccacca caaacagatt atgcttcttc gccaacgcca taatggtgtc
     9421 catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt tgtcggtgat
     9481 cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat ccacaaagac
     9541 gattttcgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt agctcggcat
     9601 gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca gcgaagcggt
     9661 gcaggatggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc actgctggca
     9721 gcgacgggta aaaccaccat cgccgcacag tttgccgcta cccattgccg actgcatata
     9781 gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat cacctgtata
     9841 accagtacgc ggtgctttct acattcgcac cactttgtat gtatcgttta agcgcggcgg
     9901 tgttgcccat ctgggtcgcc acccgcaaag ttgttttacc gcgagcatac gcccagttta
     9961 gcgccgtttg cattagctca gcacctgcac cgcgtccagc cagcaggcca attcgcgcat
    10021 ctgtcgcatt gagttcccgt aaagagacat aaccgcgaat atcgccggac gccgcacgta
    10081 aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac tgtgcataaa
    10141 agcgaccgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc gcaaatgcgg
    10201 cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactact gcaccactat
    10261 cactgacatt gttcacgggt agcgccaaat caacttcacc ttctaccagg gagaatccca
    10321 gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc tgcacccgtg
    10381 accacggcgc taacgcgtct ggcgtcagga gcggtgcttc agacgtaatg cgcacgatgg
    10441 cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg gcgcggacgg
    10501 gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga actggcagca
    10561 cgcttcacac cctcgtcatc gagccagccg ttacgccagg caatctcttc caggcaggca
    10621 atcttaaagc cctggcgttt ttctactgtc tgcacaaaag tgctggcctc aatcaggctg
    10681 tcgtgagtgc cagtatccag ccaggcaaat ccgcgcccga gcagttcaac ggtcaggttg
    10741 cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg ctccgacggc
    10801 ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc ggtcaccgcc
    10861 cagtttgatt tcggctgttt tggtttttct tccagcgaga tagcgcggaa attgtcgtca
    10921 aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaatac cgtcgcccct
    10981 tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgtccgaa gaagatgtta
    11041 tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat gataaaggcc
    11101 tgcgccagac cgtccggact gggctgttcg gcgtattcca gctggatacc gaactcacta
    11161 ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat gatgagaatt
    11221 tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg cttatcgtaa
    11281 atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt gccggaaccg
    11341 cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt cagacctaaa
    11401 cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agatttcatt agccagatac
    11461 cactgcaccg ttttacgcat tccactttca aaggtttcct gcggcagcca gccaagttca
    11521 cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg gtcagcgaca
    11581 aaggtgatca ggtcacgata gtgcgccacg ccgtgcggct tattcggagc cagttcttcc
    11641 agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg accaccaata
    11701 ttataggttt caccgacttt ccctgtggtc gccacgcaat acagcgcgcg ggcgtgatct
    11761 tcaacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag cggtttaccc
    11821 gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta aggaccgtag
    11881 ttattcgagc agttggtgat aagcgtcggc aaaccatagg tccgcagcca ggcgcgcacc
    11941 agatggtcgc tgctggcttt tgacgcggaa taagggctgc tcggcgcata cggcgtggtt
    12001 tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt ggagatatga
    12061 tgaaaacgga acgctgattt tttatcttcc gtcagcgcag tccagtaagc ccgcgccgct
    12121 tcaagcagtg tataagtccc gacaatgttg gtttcaataa acgctgccgg gccgtcaata
    12181 gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg atactcagtg
    12241 aatacgcgcg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc aaagcgctcg
    12301 ctttgcgcga ccggtgccag cgacatcagg tttccagcgt aggtcagctt atcgaccacc
    12361 accaccgcgt cgctcgtttc gttgatgata taacgcacca gcgccgagcc aataaacccg
    12421 gcaccacctg ttatcagaat ctttctcatc agcgccagac tcctttggca tcgacgacat
    12481 actgctgatg gacgttgtcg ccattgataa ctttgaactg actatgatcg accagcatca
    12541 ccagcacatc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa agcccggtca
    12601 gtttcttcgg caactcgtgg atgttaggct caacaaccag cgtttcgccg ctgtgccact
    12661 gggcgatcag ttcagcgatt tccatcgcag ggctttcacg caggtcatca atattcggtt
    12721 taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg gcagcagcca
    12781 gacaatcagc caccgccgct ttcacctgat cgataaccca gaacggtttg tgatcgttca
    12841 cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg ataaaccacg
    12901 gatcaacagc gatgcagtga ccgcccacgc cagggcctgg ctgaagaata ttgacgcgag
    12961 ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg gcacaaatca
    13021 gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg gtgagcttac
    13081 acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa attttgtaca
    13141 gttcgctggc gcgggccgaa caaaccggcg tcataccacc aatcacgcga tcgtttttaa
    13201 tcagctcgac cataacctgg cccggtaata cgcgttccgg gcagtaagca atgttgacgt
    13261 ccgccaactc gcccacctgc tgtgggaaag tgagatctgg acgcatctct gctaaccatt
    13321 ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc aacgcgcctt
    13381 ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata tctggctcat
    13441 gatcgccttt aaacggcgtg ggtacagcaa tcaaccaggc atccgcttca actggcgtcg
    13501 tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc aaatcaggct
    13561 cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg atatcgacac
    13621 caatcacctg tttttgcctt gaggcaaacg cagctgctgt aggcagcccg atgtaaccca
    13681 gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt ttaacgcttc
    13741 cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc tcatagcttg
    13801 atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct gcttatccgt
    13861 gccgaccaga cgcaccgtcc ccgctgtcac cgcttccgga cgctcagtgg tatcgcgcat
    13921 caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg agtcggtcaa
    13981 aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg gatcgatcag
    14041 aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt tcgggttgag
    14101 atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg ccagcgcatg
    14161 gcagatttct tcaaagccac gaccgaagct ctcacgcctg tgaccggtca ccagaatcat
    14221 ctttttatcg ggatcgataa acgggtaatt tgccgccagt tctgaacgca gcgtatcgct
    14281 gctcatcacc tggtcacgca ccaataacag tgcatcaata actgtattac cggtaatgaa
    14341 gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt cggttggaga
    14401 aaaatgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg gccacggcga
    14461 atagagatcg cccgtgcgca gaccagcctc aacgtgacca acaggaatac gctgataaaa
    14521 cgccgccagg ctggttgcca gcgtcgtcgt agtatcgccg tgaaccagca cgacatctgg
    14581 tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta tctctgtaag
    14641 gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa agagtttcag
    14701 cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct caaaaaaagg
    14761 atcttttgcc aacgcatgca ccaacggcgc catcttgatg gcttccgggc gcgtaccaaa
    14821 tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct ttgcgctcac
    14881 cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc ccgatcagtc
    14941 ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg cgttttaccg
    15001 gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccagg gttggaccaa
    15061 cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag gccggaccga
    15121 cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg aacatttctg
    15181 aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta tgctgctcag
    15241 caattttcag cgcctgttca atactgttca tccggcgatc gtagatggct ttcgccacct
    15301 cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat gcgcctttca
    15361 gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac tgacgtaaca
    15421 ggttattagc gtcaggcgca gtttcggcaa tcagcttcac gctgtcgttg accgcgcggg
    15481 tgaagtcacc gggaataaac acgatgttgt tgatcatttc atccagcaac gccgcatcgg
    15541 ctttgctatt gcccaccatc cgctgtttgt aatagtcggt ttgcagccag aactctctgc
    15601 gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc atgaccgatg
    15661 gttggtcggc agaagccatg tttgaacgga catccaggtt acgcaaaaat tgctgctgtg
    15721 agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgctgtc gagctccact
    15781 cctggcgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc cccatgccaa
    15841 taatccacag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc agttcatttt
    15901 cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca tttatttggt
    15961 taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct taataaagcg
    16021 agcaactttc caggcacgct tgatgcaata tccatagagg aagaatgcta gcaaaaagag
    16081 caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc caatggaagc
    16141 gagcagtgct gcggcaaggg taatcagcac aaacgcctgg cgggaagtaa acccggcacg
    16201 catgatcaaa tggtgaatat gctgacggtc tggagagaat gggctcatgc ctttacgcag
    16261 gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc acaaagcggt
    16321 aaccgggctg atgggatgag ttttgccctg ggtcgtttcg agcaggatcc agataacggt
    16381 aaaaccaatc agcgtactgc ccgcatcccc cataaagact ttgtagcggc gacccaggat
    16441 accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc accagattgc
    16501 gagactggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg agacgcagga
    16561 caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa tggccgccca
    16621 gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc aggagccaaa
    16681 gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa tgccaacagc
    16741 ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca gcgcgccaat
    16801 gaaaacaagc acaccagcac aagcgagata gagagatgca tgcggaatat agtaatcgac
    16861 aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga gaggtatcaa
    16921 tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt ttgccacctt
    16981 acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat cagtactcac
    17041 tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt ataaccacga
    17101 agacctttat tcagaaagga aagtccgttc cgaacctacc agcactatgc gaaaattaac
    17161 tacttgatat gcaaattgca taacacacca gcaaattcca ggaattttcc taacatcgcc
    17221 agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg tggcgttttt
    17281 ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt ggtcttggtc
    17341 attgccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg gtgaatgatt
    17401 ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc ttctttacgg
    17461 gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat cttacgagag
    17521 aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt agataacttc gtccatttta
    17581 gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc ttccacgtta
    17641 cgcgccgcac cgaagaagcg tttcggacga tgcagggcgt tggcgtccac accaccggtc
    17701 aacactttac ctgacgccgg aacaacggtg ttgtaagcgc gcgccagacg agtgatggag
    17761 tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttcgcctt ctcgatcacc
    17821 atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc aacaacttca
    17881 ccttttacca gtcgctgcat ctcggttact tcttccggac gttcgtcgat cagcagaacc
    17941 atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg cagcagcatg
    18001 gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc gataggtgat
    18061 gccagatcca gtacgcgagc ggttaaatct tccgtagaac cgttaccacg ttccatacgc
    18121 agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt gcgggcgttt
    18181 tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg ttcaccttct
    18241 ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa acggcggatt
    18301 tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc tgcggaacgg
    18361 aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat atcttcgcca
    18421 ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat acgagccagg
    18481 ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt attctttaat
    18541 tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga acttaatgtt
    18601 gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat gtcttttcgc
    18661 tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag tgacgagcca
    18721 gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta caagattcaa
    18781 acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc acgccgggcg
    18841 tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg ttagcgtcga
    18901 ggaactcttt caactgacct ttagacagtg cgcccacttt ggttgccgcc acttcaccgt
    18961 ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca gtgccagggt
    19021 tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca gcgatttcat
    19081 ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa tcgacgagga
    19141 tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg tgaataattt
    19201 tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt tgttggtgta
    19261 acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa agcaatagta
    19321 agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag ttttccgact
    19381 tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat aactgcacgc
    19441 ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc gggcaggcgc
    19501 aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat cttctctctc
    19561 atcccgcgat tgccgatcgc aaggtgaatc agccgcgtgc cttaattatg gcaccgacgc
    19621 gtgaacttgc cgtgcagatc cacgccgacg cggaaccgct ggcggaagct accggcctga
    19681 agctgggtct ggcttacggt ggtgatggct acgacaagca gctgaaagtg ctggaaagcg
    19741 gcgttgacat tctgattggc accacgggtc gtttaattga ctacgccaag cagaaccaca
    19801 ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg tacgatctgg
    19861 gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac cagcgcctca
    19921 acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc gagcagatga
    19981 acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt attaaagaag
    20041 agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg atcgaagaag
    20101 agtggccaga cagagcgatt attttcgcca acaccaaaca ccgttgtgaa gagatctggg
    20161 gccacctggc ggcagatggt catcgtgtcg gtttattgac aggcgatgtc gcgcagaaaa
    20221 aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg gttgccaccg
    20281 acgttgccgc gcgtggtttg catattcctg ctgtgacaca cgtctttaac tacgatttac
    20341 ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca ggcgcaagcg
    20401 gtcactctat tagcctggcg tgtgaagagt atgcattgaa tttgcctgct attgagacct
    20461 atattggtca ctcaattccg gtaagtaaat acaatccgga cgcattgatg accgatctgc
    20521 caaaaccgct gcgcctcacg cgcccgcgca caggcaatgg tccgcgtcgt actggcgctc
    20581 cgcgtaatcg tcgtcgttca ggttaaagaa aaaatgtcat actccaaatc actcaagctg
    20641 tatcaagatg gcaagagaat gaatcccgat gcgcttactg tcgtaagtga ttcaggtgaa
    20701 ggaatgcagc caacgcagag acagattgaa ggatgaagag tatgggttcc acctcgtcgc
    20761 tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg cgcgaggtgg
    20821 ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct gccggcctga
    20881 atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt
    20941 ttgctgaacg tctgcaagat atccctccct cgcaaattcg cgttgtcgct acggcgacat
    21001 tacgacttgc cgtcaatgcg ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc
    21061 cggtacaggt gatcagcggt gaagaggaag cgcgtctgat ttatcagggc gttgctcaca
    21121 ccactggcgg tgccgatcag cgcctggtgg tggatatcgg cggagccagt actgaactgg
    21181 taaccggcac gggtgcacaa accacctctt tgttcagcct gtcgatgggc tgcgttacct
    21241 ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat gctgcagaaa
    21301 aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac ggctggaaag
    21361 tgtgcgttgg cgcttccggc accgtgcagg cgttacagga aatcatgatg gcacagggga
    21421 tggatgaacg cattaccctg gaaaagttgc agcaactgaa acaacgcgcc attcattgcg
    21481 gtcggctgga agagctagag attgacgggc tgacgctgga acgtgcgtta gtgttcccga
    21541 gtggtctggc gatcctgata gccattttta ccgaactgaa tattcagtgt atgaccctgg
    21601 cgggcggtgc gctgcgtgaa ggcctggtct acggtatgtt gcatcttacc gtcgagcagg
    21661 atattcgcag ccgtacgctg cgtaatattc agcgccgctt tatgatcgat attgatcagg
    21721 cacagcgcgt agccaaagtt gcggctaact tctacgatca ggtggaaaat gaatggcatc
    21781 ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa ataggcctga
    21841 gcgttgactt caaacaagcg ccgcaacacg ctgcttatct ggtacgtaat ctggatcttc
    21901 ccggttttac ccccgcacag aaaaaactgc tggcgacgct actgctcaac cagactaatc
    21961 cggtcgatct ctcatcgctg catcagcaaa atgccgtacc accgcgcgtc gcagaacaac
    22021 tctgccgttt actccgcctg gctatcattt ttgccagccg tcgccgtgac gatctcgtgc
    22081 cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg caaggttggc
    22141 taacccaaca tccgctgggt aaagagatta ttgatcagga aagccagtgg cagagctatg
    22201 tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct gacacatctt
    22261 ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat tcgccagatg
    22321 gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct gttcccaaat
    22381 caaatcatcc tgcggcagct ccagcaggaa gcggctcggc tccgggcgca ccagttcgcc
    22441 gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc
    22501 gacataggcc agccgccgct cctcatcgat attatcttca tcgatactgc tctggtgggg
    22561 caaaaatcct tcttccatac caaccatgta gacataggga aactccagtc cttttgacgc
    22621 gtgcaaagtc atcagttgca cctgatccag ctcttcctcg ctctcgccgc gctccatcat
    22681 gtcgcgcaga gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat ccagttcgct
    22741 gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca tgcgcatttc
    22801 ggcagctttc gggctgggcg atgtttcgta cagccaggat tcataatcca tgccgtggat
    22861 cagatcgcgc accgcggcaa tcggctcccg ctccgccaga cgctggattt ctgccagcca
    22921 gtgagtgaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac tcaggcccat
    22981 atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccattcac ctagcttttt
    23041 cagcgtcgcc gggccaatct ctcgcttcgg cgtgttaacg atacgcagaa atgcgctgtc
    23101 atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt caggacgaga
    23161 gaaaaacgac gtaccaccag aaattttgta cgggatgcgg ttttgcatca ggaatttttc
    23221 aaatacccgc gactgatggt tgccgcgata aagaatggcg taatctttgt actgcgtttt
    23281 attgacgaag tgatgggcga tcagctcgcc ggtgacgcgc tcggcttcat gttcctcgtt
    23341 attcgcgctt aaaactttta gctctgtgcc gtaacccagt tcggagaaca gacgcttttc
    23401 aaagacgtgc gggttattgg cgatcaggat gttcgccgct ttcaaaatac gtccggaaga
    23461 gcggtagttc tgctcaagct taatcacctt cagcgtcgga aaatcctggc tcagcagcac
    23521 cagattctgc ggacgtgcgc cgcgccagga gtagatcgac tggtcatcgt cacccaccac
    23581 ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc tggtgttggt
    23641 gtcctgatac tcatccacca gcaggtagcg aattttgttc tgccagcgct cgcggacttc
    23701 ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt cgagaacgtt
    23761 acaggctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac ggtcccgctc
    23821 gccctttgcc tctgccgccg cctgcgccgg tgttttgaga tcgttcttcc agttggagat
    23881 ggtcgaaatt agctgttgca ggagaacttt gtcatcttca atcagcccct cggtcaactc
    23941 tttaagcaaa gcaagctgat cggtatcgtc aaacagcgaa aagttagcct tcatccccag
    24001 cgccgcatac tcgcgtttga tgatatccag ccccagcgtg tggaaggtgg agatcatcag
    24061 cccacgcgcc tctttgcgcc ccagcgtctg cccgacacgc tctttcatct cgcgcgccgc
    24121 cttattggta aaggtcaccg ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag
    24181 gtgggcgatt ttattggtga taacacgagt tttaccggaa cccgcgcccg ccagcaccag
    24241 gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac gcataggaaa
    24301 atcgctcaat gaaagtcggg aggggaaaat aacagcgtgg tagtatagcc agcctcatcc
    24361 ttaccactca aggcacgatc atggcaaaaa cagcagcagc actgcatatc cttgtaaaag
    24421 aagagaaact ggctctggat cttctcgagc agatcaagaa cggtgccgat ttcggcaagc
    24481 tggcgaagaa acactccatt tgcccgtcag gcaaacgcgg cggtgatttg ggtgaattcc
    24541 gccagggtca aatggttccg gcgttcgata aagtggtttt ctcttgtccg gtactggagc
    24601 caaccggccc gctgcatacc cagttcggtt accacatcat taaggtgctg taccgtaact
    24661 aacagcaagg ccttctccgg gagaaggcct tgaactctga atcgctatta acccgcaaca
    24721 gcaatacgtt tcatatccgt catatagccg cgcagtttct tacctacctg ctcaatcgca
    24781 tggctgcgaa tcgcttcatt tacatcacgc agctgcgcgt tatctaccgc accttccgga
    24841 atagctttac ccaggtcgcc cggttgcagc tctgccataa acggtttcag cagtggcaca
    24901 caagcgtaag agaacagata gttaccgtac tcggcagtat cggagataac cacgttcatt
    24961 tcgtacagac gcttacgggc gatggtgttg gcaatcagcg gcagttcgtg cagtgattca
    25021 tagtaagcag attcttcgat gatgccggaa tcaaccatgg tttcaaacgc caactcaacg
    25081 cctgctttta ccatcgcgat catcagtacg cctttatcga agtactcctg ttcaccgatt
    25141 ttgccttcat actgcggcgc ggtttcgaat gcggttttgc cagtctcttc acgccaggtc
    25201 agcagtttct tatcgtcgtt ggcccagtca gccatcatgc cggaggagaa ttcgccggag
    25261 atgatgtcgt ccatatgttt ctggaacagc ggcgccatga tctctttcag ttgctcagaa
    25321 agcgcgtaag cacgcagttt cgccgggtta gaaagacggt ccatcatcag ggtgatgccg
    25381 ccctgtttca gcgcttcggt gatggtttcc caaccgaact gaatcagttt ttctgcgtat
    25441 gccggatcgg tgccttcttc caccagcttg tcgaagcaca gcagagaacc agcctgcaac
    25501 ataccgcaca gaatggtttg ctcacccatc aggtcagatt tcacttccgc aacgaaggac
    25561 gattccagca cgcccgcacg gtgaccgccg gttgcagccg cccatgcttt agcgatcgcc
    25621 atgccttcgc ctttcggatc gttttccggg tgaacggcaa tcagcgtcgg tacgccgaat
    25681 ccacgtttgt actcttcacg tacttcggtg ccagggcatt tcggcgcaac cattacgacg
    25741 gtgatgtctt tacggatctg ctcgcccact tcgacgatgt tgaaaccgtg tgagtaaccc
    25801 agcgccgcgc cgtctttcat cagtggctgt acggtgcgca ctacatcaga gtgctgcttg
    25861 tccggcgtca ggttaaccac cagatccgcc tgcgggatca gttcttcgta agtacccact
    25921 ttaaaaccat tttcggttgc tttacgccag gatgcgcgct tctcagcaat cgcttcttta
    25981 cgcagagcgt aggagatatc gagaccagaa tcacgcatgt tcaggccctg gttcagaccc
    26041 tgtgcgccac agccgacgat gactactttt ttaccctgaa ggtagctcgc gccatcggcg
    26101 aattcatcgc gccccataaa gcgacatttg cccagctgtg ccaactgctg gcgcagattc
    26161 agtgtattga agtagttagc catggtgatt cctcgtgatg ttgtgcttct tattgttcgg
    26221 ttcgcgtttg cgagattgaa ttcactatat gacaggaaat ttattgcgga aattgatata
    26281 ttcacaacgt cacattgcaa tttctgcaac gtcaacatta agggctgcgc cattggattt
    26341 acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc cattttggcc gcagcgcgcg
    26401 ggcaatgcac gttagcccgt ccacgctctc tcggcagatt cagcgcctgg aagacgatct
    26461 cggtcagccg ctgtttgtgc gcgataaccg cacggtaacg ctaaccgaag caggcgaaga
    26521 actgcgcgtt ttcgcccagc aaacgctgtt gcagtatcaa cagctgcgcc acaccatcga
    26581 tcagcaagga ccgtcgctct ctggcgaatt acatatcttc tgctcggtga ccgctgccta
    26641 cagccatctg ccgccgattc tcgatcgctt ccgcgcggaa cacccgtcgg tggagattaa
    26701 actcacgact ggtgatgcgg cagatgcgat ggaaaaggtc gtcactggcg aagcggatct
    26761 ggcgattgcc gggaaaccgg agactctgcc cggcgcagtg gcattttcga tgctggagaa
    26821 tctggcagta gtgctgattg cccctgcgct accctgcccg gtgcgtaacc aagtttcggc
    26881 agataagccg gactggtcta cagtgccgtt tatcatggcc gatcagggac cggtgcgccg
    26941 ccgcattgaa ctgtggtttc gacgcaacaa aatcagcaac ccgatgattt acgccacggt
    27001 cggcgggcat gaagcaatgg tctcgatggt ggcgctcggc tgcggcgtgg cattgttgcc
    27061 ggaagtggtg ctggaaaaca gcccggaacc ggtgcgtaac cgcgtgatga ttttagagcg
    27121 cagtgatgaa aaaacgccgt ttgagctggg cgtgtgcgta caaaaaaagc ggcttcatga
    27181 gccgctgatt gaggcgttct ggaagatttt gccgaaccac taacccgcca aaaagaacct
    27241 gaacgccggg ttattggttt cgtcgtggca atcgtagccc agttcattca gccgggtttc
    27301 aaaatccggt tcatgatcgc caagctcgaa cgccgccagt acgcgcccgt agtcggtgcc
    27361 atggctgcga tagtggaaca aagaaatgtt ccagtgcgta cccagcgtgt tgaggaagcg
    27421 caacagcgcg cccggtgatt ccgggaattc gaagctgtag aggcgttcct gcaacggatg
    27481 cgatggacgc ccgccaacca tatagcgcac atgtagcttc gccatttcgt cgtcggagag
    27541 atcaaccacg ctgtagccgc cgtcgttgag catctgcaaa atttctttgc gctcttcgag
    27601 accgcggctc aggcgcacgc cgacaaaaat gcaggcgttt ttggcatcag caaagcggta
    27661 gttgaactcg gtgaccgagc gcccaccaag taactggcag aatttgagga agctgccctt
    27721 ttcttccgga atggtcaccg ccagtaacgc ttcacgctgt tcgcccagtt cgcagcgttc
    27781 cgagacgtag cgcagaccgt gaaagttcac gttggcaccg gaaagaatat gcgccagccg
    27841 ttcaccgcga atgttgtgtt gggcgatgta ttttttcatc cccgccagcg ccagcgcgcc
    27901 ggagggttcc gccaccgcgc gcacatcttc gaacaggtct ttcatcgccg cacatatggc
    27961 atcgctatcg acggtgatga tgtcgtcgag atactcctgg cataaacgga aggtttcatc
    28021 gccgatgcgt tttaccgcga cgccttcggc gaacagcccc acacgcggca gatcaacagg
    28081 atgaccggca tccagcgccg ctttcagaca ggcggagtct tccgcttcca cggcgatcac
    28141 tttgatttgc ggcatcagtt gtttgatcaa caccgccacg cccgccgcca ggccgccgcc
    28201 gccgaccggc acaaatacgc ggtcgagatg agcatcctgc tggagtagtt ccagcgccag
    28261 cgtcccttgc ccggcgatca ccatcggatg gtcgaacggc ggcacccagg tgaagccctg
    28321 ctgctgtgac agttcgatcg ctttcgcttt tgcttcatca aagttcgcgc cgtgaagaag
    28381 cacttcgccg ccgaagccgc gcaccgcgtc gactttgata tcggcggtgg cggttggcat
    28441 gacgatcagt gccttcacgc ctaaccgtgc ggaagaaaac gcgacgccct gcgcgtggtt
    28501 acccgcagaa gcggtgatca cgccgtgcgc tttctgttct tccgtcaggc ccgccatcat
    28561 ggcgtatgcg ccgcgcagct taaagctatg aactggctga cggtcttcgc gtttcaccag
    28621 aatcacgtta tcgagacgcg acgagagttt ttccattttt tgtagcggcg tgacctgcgc
    28681 cgcttcgtag accggcgcgc gcagcaccgc tcttaaatat tcggcacctt ccggggcacc
    28741 ggacaggggt tgtgagtctg ccatcattaa ccccccagtt tcgatttatc acgcaccgcg
    28801 cctttgtcgg cgctggttgc caggctggcg taggcacgca gcgcaaagga aacctgacgt
    28861 tcacgatttt tcggtgtcca ggctttgttg ccacgagctt cctgcgcttc acgacgcgcc
    28921 gccagttcag catcgcttac ctgtaactga atgccacggt tcggaatgtc gatagcgatc
    28981 aggtcaccat cttcaatcag gccaatgctg ccgccacttg ccgcttccgg tgagacgtgg
    29041 ccgatggaaa gaccagaggt gccgccagag aaacgaccgt cggtgatcag cgcacaggct
    29101 ttaccgagac ccattgattt caggaaactg gttgggtaga gcatttcctg catccccgga
    29161 ccgcctttcg ggccttcata gcgaattact accacatctc cggcgacaac tttaccgccg
    29221 agaatcgctt ctaccgcgtc gtcctggctt tcgtagactt tcgccgggcc agtgaatttg
    29281 aggatgctgt catcgacgcc tgcggtttta acgatgcagc cgttttctgc gaaattaccg
    29341 tagagcaccg ccaggccgcc gtctttgctg taggcgtgtt ccagcgagcg gatacagcca
    29401 ttggagcgat cgtcgtccag cgtatcccag cggcaatcct gcgagaatgc ctgtgtagta
    29461 cgaatgcccg ccgggcctgc gcggaacata tttttaaccg cgtcatcctg ggtcagcata
    29521 acgtcgtatt gttccagcgt ttgcggcaac gtcaggccaa gcacgttttt cacatcacgg
    29581 ttcagtaacc ccgcgcgatc cagttcgcca agaataccga taacaccacc agcacgatga
    29641 acatcttcca tatggtattt ttgggtgctc ggcgcaactt tacacagctg cggaaccttg
    29701 cgggaaagct tatcgatatc actcatggtg aagtcgattt ccgcttcctg cgccgccgcc
    29761 agcaggtgaa gtacggtgtt ggtagagccg cccatcgcga tatcgagcgt catggcgttt
    29821 tcaaacgccg ccttactggc gatattacgc ggcagtgcac tttcgtcatt ttgctcgtag
    29881 taacgtttgg tcaattcaac aatgcgctta ccagcattga ggaacagctg cttacggtcc
    29941 gcgtgggttg ccagcagcga gccgttgccc ggctgcgaca ggcccagtgc ttcggtcagg
    30001 caattcatag agttagcggt aaacatcccg gagcaagatc cgcaggttgg acaggcggaa
    30061 cgttcaacct gatcgctctg ggagtcagaa actttcgggt ctgcgccctg gatcatcgca
    30121 tcaaccagat cgagcttaat gatctgatcg gacagtttgg ttttcccggc ttccatcggg
    30181 ccgccggaaa caaagatcac cggaatattc aggcgcaggg aagccatcag catccccggg
    30241 gtgattttgt cgcagttaga gatgcagacc atggcgtcgg cgcagtgagc gttgaccata
    30301 tactcaacgg aatcagcgat cagttcgcga gatggcagcg aataaagcat ccccccgtgg
    30361 cccatggcaa tcccatcatc caccgcaatg gtgttgaact ctttggcaac gccgccagcc
    30421 gcttcaattt gttcggcgac cagtttaccg agatcgcgca gatggacgtg acccggtaca
    30481 aattgggtga acgagttcac aaccgcgata atcggcttac cgaaatcggc gtcggtcatt
    30541 ccggtggcgc gccacagcgc acgagcaccc gccatattac gaccatgagt ggtggtggcg
    30601 gaacggtact taggcatact ttatttactc ccagtgtctg tctcgtaaat gggacggtgc
    30661 gtgccgtccc attttttgta tttattgatt aacttgatct aaccagcccc atttatcttc
    30721 ggtttcgcca gtgaagaggc cgaagaaggc ttgctgaatg cgtttggtaa ccgggccaca
    30781 acggccttcg ccaacctgaa taccgtctac gctgcgcact ggcgtgattt ctgccgccgt
    30841 accggacata aacacttcat ccgccaggta cagggattcg cgcgacagca cctgctcacg
    30901 cacttcaatt cccagctctt ttgccagttt gatgatggca tcacgggtaa tacccggcag
    30961 cgcggatgag gtgaacggtg gggtgaacag cacgccgtct ttcacttcaa acaggttttc
    31021 gcctgcgcct tcagagatgt aaccattcac atccaacgcg ataccttcct gataaccgtg
    31081 gcggcgcgct tcgctaccca ccagcaggga agagaggtag ttaccaccgg cttttgccgc
    31141 cgtcgggatg gtgtttggtg ctgcgcggtt ccaggaggaa accatcgcat cgatcccctg
    31201 ctccagcgct tctgcgccca gatacgctcc ccacgggaaa gcggcgataa tcacgtcggt
    31261 tgagtatccc gctggcgggt ttacgcccat gccaacatca ccaacgaaga tcagcggacg
    31321 gatataggcg ctggtgagat tgtttttgcg gatcacgtca cgacaagctt ccatcagctc
    31381 atcaatgctc tgcgaaaccg ggaagcgata gattttggcg gagtcatgca gacgctgcat
    31441 atgctcacga tggcggaata caaccggtcc tttgtgcgag tcgtagcaac ggatgccttc
    31501 aaaaaccgag gtgccatagt gcagcgcgtg cgacatcaca tgcaccttcg cgtcttccca
    31561 gcgaaccatc tccccattga accaaatgta atcagctttc ttcgtggtca tttttatatt
    31621 ccttttgcgc tcaggcgcgg atttgttgtg atgtggttgt gctctggcag atagcaacgt
    31681 gtgcgacgtc caccagttta tttaactgac taaacagtaa gtcgaccgac cgtgggctgg
    31741 caacggtcaa ttcgatattt atattttgtg catcgctggc ggcggccata ttcattgagc
    31801 agacgtggaa accacgatga cgcaccacgc gtaaaacacg ttctaaggtt tccggattga
    31861 agcgagccga tacattgacc tgatgttgca tcatgataat ttctccaaca tttctgaatt
    31921 actggcacct ggcggcacca gcggccagac gttctcaagt tcgtcgattg agacatgaag
    31981 caggtatggc ccatcactgt tcagcatggt gtcgagtgcc gcttcaacct ggtctttacg
    32041 ggtgatgtgt tggccaggga tgccgaaggc gctggctaac atgaggaaat cggggttatc
    32101 ggtaagggtt gtttcgctgt atcgttcctg gaaaaacagt tgctgccatt gtcgaaccat
    32161 ccctaaccgt tggttatcga gtaagacgat tttcaacggt aactgcttgc gttttacggt
    32221 gcccagctct tgcacattca tcatgaaaga gccgtcaccg gagatacaga cgacggtatc
    32281 gttcggtcgc gcgacttgtg cgccaaccgc cgccggtaaa ccaaaaccca tggtgcctaa
    32341 gccgctggag gtgatgaaat tttccgggcg agtgtgggcg atgtgctgcg cagcccacat
    32401 ctggtgctgc cccacatctg tggtcacgac gcaatccgca ggttttcgat ccgacagttg
    32461 ttttaacaac aacggcgcgt agatagcgtc accgggatgg tcgtaacgcc aggtatgttc
    32521 atcacgcagc tgcgcgcagt attgctgcca gtcattgata tttaacggct gctgtaatgc
    32581 tggtaacaga gcatttaaat caccctgtaa tgccacatgt gcctgacgca gcttgttcat
    32641 ttctgccggg tcgatatcca tatggataac gctggcgtgt ggtgcgaagg tgttcagttt
    32701 gccagtcacc cggtcatcaa aacgtgcacc cacggcgatc agtagatcgc actcctgcac
    32761 tgcgaagttc gccgctttgg tgccgtgcat ccccagcatg cccagatagt acggataatc
    32821 tgcttctact gcgcccagcc ctttcaacgt acaggtggca ggcatttttg tggcagcgag
    32881 aaattcacgc aaagccgaaa ctgcctgcgc catgcccacg ccaccgccaa cgtacagcat
    32941 cggtttttgc gcttttgcca gcatctgacg cgcttgctcg acttcggcat gtgggaaagt
    33001 cacttcgttt tcaacagtgg tgaaccacgg ttccagatcg ccgctggcta actggatatc
    33061 ttttggaata tcgaccaaaa ccggaccagg acgaccagag ctggcgacgt caaacgcttc
    33121 ggccatgata cgcggtaact cttccagtga ttgcaccagg aaactgtgct tggtgcaggc
    33181 taacgacaac cccagaatat ccacctcctg aaatgcgtca gtgccgataa acggtgcgga
    33241 tacctgaccg gtgatagcaa caacggggat ggaatctaac agcgcgtccg caagcccggt
    33301 tatcaggttg gttgcacctg ggccagacgt ggcgatgcaa acgccagttt tgccggtagc
    33361 acgggcataa ccgatagccg ccattgctgc accttgctcg tgtcggcaca gcaagtgttc
    33421 cacgccaccg tcatacaatg catcgtaaac cggcataatt gcgccacccg gataaccgaa
    33481 aacggtgttt acaccctgtg cccgcaacgc atgtaccacc cactgtgcgc cattcatagt
    33541 tagttccccg tcctgaatct tgagaaacag aattttgtgc tgttattcat tgtctgctcc
    33601 tcagttatgt ttttaaggtc aaaaaaaacc cccggacctt tcggtgcggg ggtcttagtt
    33661 cgttaaggct tgatctctaa gcctttcctc gtccaagtgc agccccgcac ggtgggataa
    33721 taatcaccac cacgctaatc acgaccaggc taatcactcg tagaagggct gtcattttgt
    33781 cttttcttgc atcttgttcg aaggaatgcc taaagagtta ccatagattt tgcgaatagc
    33841 acaagatatt ttttggccga tttttatgat gtctttaata atttcaagag aaattgttgt
    33901 tttttaaaaa gaaaatagtg agtgaaaaaa attcaatttc agtatgaatg ttgttccctt
    33961 cctcgtagtt catcctttgc acgctgcatt tttgcgagca tcattccacc gccagaacaa
    34021 ctcaacttca cttgcacaaa taaacaggaa tcagccacta aaattcacga attagcgggc
    34081 tacagcagcg atatcttatg cacataatgg tgactcaagg agggcttatg tcactgtcaa
    34141 ttgttcatac ccgcgcagcc ctgggagtaa atgcgccccc aatcactgtt gaggtacata
    34201 tcagtaaagg tctaccaggc ttaacgatgg tgggcttacc agaaacaacg gtaaaagaag
    34261 ctcgcgatcg cgtgcgcagc gccattatca atagcggata tgaatatccg gcgaaaaaaa
    34321 tcaccatcaa ccttgctcca gccgatctgc caaaagaagg gggacgatat gatttgccta
    34381 tcgctattgc gttactggcg gcctcagaac agcttacagc caataagtta gatgaatatg
    34441 aattagtcgg agaacttgcg cttacaggcg ctctgcgtgg cgttcccggc gcaatctcca
    34501 gtgcaactga agctattaag tcaggcagaa aaattatcgt cgcgaaagat aacgaagatg
    34561 aagtggggct aattaacggt gaaggatgcc tgatagccga tcatctgcag gctgtctgcg
    34621 cgtttctgga aggtaagcac gctctcgaac acccgaaacc aactaatgca gtatcccggg
    34681 cgctacaaca tgatctcagt gatgttgtcg gtcaggagca aggaaagcga ggactggaaa
    34741 ttaccgccgc tggcgggcac aaccttttac tgattggacc gccgggaaca ggtaaaacaa
    34801 tgctcgccag ccgtattaat ggccttttgc cagatttaag caatgaagag gcactggaga
    34861 gcgctgcgat attaagtctg gtaaatgctg aatcagtaca aaaacaatgg cggcagcgcc
    34921 cgttccgctc acctcatcac agtgcgtcgt taactgcgat ggtgggcggt ggtgcaattc
    34981 cagggccagg tgaaatttcg ctggcgcata acggcgtgct ttttcttgat gagctacctg
    35041 aatttgaacg gcgtacactg gatgccttgc gagagccgat tgaatccggg cagatccatc
    35101 tttcacgcac acgagcaaaa ataacctatc cagcccgttt ccagcttgtc gcggcgatga
    35161 atcccagccc taccggacat tatcagggaa accataaccg ctgcacacca gaacagacat
    35221 tacgttatct caaccggctc tcggggccct ttctcgaccg cttcgatctc tcactggaga
    35281 tcccattacc gccccccggc attttgagta aaacggtagt gccgggagaa aacagcacca
    35341 ccgttaaaca acgtgtaatg gccgccagag agcgccaatt taagcggcag aataaactga
    35401 acgcctggct ggataatccg gaaatacgcc aattctgcaa gcttgaaagc gaagatgcgc
    35461 agtggctgga agaaacgctg atccatctgg ggttatcgat tcgtgcctgg cagcggttat
    35521 tgaaagttgc acgaaccatt gctgatattg atcagtctga cattatcaca cgtcagcatt
    35581 tgcaggaggc agttagctat cgtgcgattg accgtttgct catccatctg caaaaactac
    35641 tgacataaaa aaagggcatt tcgccctttt tattaatcgt cagaatcggt gtagtcttca
    35701 gcaccttcaa cctgcggttt accaccggaa agggtgtgaa aacgttttgg acgcttgata
    35761 cgcgtcatat acttggacca cacgcgttct gcttctgtca ctggttcacg ttcgccacgg
    35821 catactgcta cgaagagttt ctcttcctcg gtaaccggct cgcgtttgcc aagatccaac
    35881 tcattgaagg cataaccatg acgctcaagc agttgtgcct ctttgatggt gaagtcacca
    35941 tgacgagaga atccacgtgg ataatgttta ttgtcgaaat atcgattagt cgtcgtaaag
    36001 ctttccgcca tcctgcacgc tcctaattct ttgaccgagc tagttatggc gcggagtatt
    36061 agttacgctt gacagagtgt aaaacaaaac atttaaatca taacgacaaa taattttgcg
    36121 gagagcactg tggatacgga attgttaaaa actttcctgg aagttagccg aacgcgtcac
    36181 tttggtcgag cggctgaatc gctctatctg acccaatcag ccgtgagctt tcgaatcaga
    36241 caactggaaa atcaactggg tgtgaatctt ttcacccgcc acagaaacaa tatccgttta
    36301 accgctgccg gagaaaagct tctgccttat gcagaaacgc tcatgagcac ctggcaggcc
    36361 gcccgcaagg aggtggcgca tacctcacga cataatgagt tttctatcgg tgccagcgcc
    36421 tcgctgtggg agtgtatgct taatcagtgg ctgggacgct tgtatcaaaa tcaggatgtc
    36481 catacaggct tacagttcga agcgcgaatt gcccaacggc agtctctggt aaaacagttg
    36541 catgaacgcc agcttgatct tcttattaca actgaagccc ccaaaatgga cgaattttgt
    36601 agtcagttgc tggggtattt cactttagcg ctttatacca gtgccccttc taaactaaag
    36661 ggagatctta actatctgcg actagagtgg gggccagatt ttcaacagca tgaggcaggt
    36721 ttgatcggtg ctgacgaagt tcccattctg acaaccagtt ctgctgaact ggcacagcaa
    36781 cagattgcga tgcttaatgg ttgcacctgg ctacccgtca gttgggcacg taaaaaaggc
    36841 ggcctgcata ccgttgtcga tagcacaaca ctttcacggc cgctttatgc catatggcta
    36901 caaaatagcg ataaaaatgc gttgattcgc gatctattga aaattaacgt gctggatgaa
    36961 gtgtattaat ctgaatggct ggcaaggatg ccggtagaag gagttacttc ggagagggtt
    37021 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggcag gggcggagag
    37081 actcgaactc ccaacacccg gttttggaga ccggtgctct accaattgaa ctacgcccct
    37141 aattagggtg gcggaacgga cgggactcga acccgcgacc ccctgcgtga caggcaggta
    37201 ttctaaccga ctgaactacc gctccaccga attcttttac aaccaccggt ttataaccgg
    37261 cttac
//
LOCUS       DAZGJT010000039        37008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZGJT010000039 DAZGJT010000000
VERSION     DAZGJT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.039"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..209
                     /locus_tag="ACHKFK_002128"
     CDS             <1..209
                     /locus_tag="ACHKFK_002128"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052465.1"
                     /translation="QDFFSLLLRPSCEVFHVVVSTEAHYRDPNFLHKYFFDLFFCLLL
                     FHPFCCIRTQNGAFLHAKRLAQGQ"
     gene            253..807
                     /locus_tag="ACHKFK_002129"
     CDS             253..807
                     /locus_tag="ACHKFK_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO9052466.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            complement(783..1040)
                     /locus_tag="ACHKFK_002130"
     CDS             complement(783..1040)
                     /locus_tag="ACHKFK_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO9052467.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(1037..1855)
                     /gene="aroE"
                     /locus_tag="ACHKFK_002131"
     CDS             complement(1037..1855)
                     /gene="aroE"
                     /locus_tag="ACHKFK_002131"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO9052468.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVAITNRTVSRAEELTK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            complement(1860..2432)
                     /gene="tsaC"
                     /locus_tag="ACHKFK_002132"
     CDS             complement(1860..2432)
                     /gene="tsaC"
                     /locus_tag="ACHKFK_002132"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO9052469.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            complement(2437..2979)
                     /locus_tag="ACHKFK_002133"
     CDS             complement(2437..2979)
                     /locus_tag="ACHKFK_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO9052470.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPISAE"
     gene            complement(3008..3481)
                     /gene="smg"
                     /locus_tag="ACHKFK_002134"
     CDS             complement(3008..3481)
                     /gene="smg"
                     /locus_tag="ACHKFK_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO9052471.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            complement(3453..4577)
                     /gene="dprA"
                     /locus_tag="ACHKFK_002135"
     CDS             complement(3453..4577)
                     /gene="dprA"
                     /locus_tag="ACHKFK_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO9052472.1"
                     /translation="MVDTEIWLRLISISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHAPLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            4707..5216
                     /gene="def"
                     /locus_tag="ACHKFK_002136"
     CDS             4707..5216
                     /gene="def"
                     /locus_tag="ACHKFK_002136"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO9052473.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            5231..6178
                     /gene="fmt"
                     /locus_tag="ACHKFK_002137"
     CDS             5231..6178
                     /gene="fmt"
                     /locus_tag="ACHKFK_002137"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO9052474.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNIVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEDKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTTTKAAPGTILEANKQGIQVA
                     TGDGILNLLSMQPAGKKAMSVQDLLNSRREWFVPGNRLA"
     gene            6230..7519
                     /gene="rsmB"
                     /locus_tag="ACHKFK_002138"
     CDS             6230..7519
                     /gene="rsmB"
                     /locus_tag="ACHKFK_002138"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO9052475.1"
                     /translation="MKKQRNLRSMAAQAIEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAVAIKRPQLKGLINGVLRQFQRQQDELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVQLETPAPVH
                     ALPGFEEGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVLAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWSHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            7541..8917
                     /gene="trkA"
                     /locus_tag="ACHKFK_002139"
     CDS             7541..8917
                     /gene="trkA"
                     /locus_tag="ACHKFK_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO9052476.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            9047..9460
                     /gene="mscL"
                     /locus_tag="ACHKFK_002140"
     CDS             9047..9460
                     /gene="mscL"
                     /locus_tag="ACHKFK_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO9052477.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLREAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAATPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(9457..9675)
                     /gene="arfA"
                     /locus_tag="ACHKFK_002141"
     CDS             complement(9457..9675)
                     /gene="arfA"
                     /locus_tag="ACHKFK_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO9052478.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSDAC"
     gene            complement(9731..10156)
                     /gene="zntR"
                     /locus_tag="ACHKFK_002142"
     CDS             complement(9731..10156)
                     /gene="zntR"
                     /locus_tag="ACHKFK_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO9052479.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTKSD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDTCCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            complement(10167..10535)
                     /locus_tag="ACHKFK_002143"
     CDS             complement(10167..10535)
                     /locus_tag="ACHKFK_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO9052480.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLVGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELENRREAIQLLDILKGIRHDDPQYQEVSRRLSLLQLKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            complement(10642..11025)
                     /gene="rplQ"
                     /locus_tag="ACHKFK_002144"
     CDS             complement(10642..11025)
                     /gene="rplQ"
                     /locus_tag="ACHKFK_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO9052481.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            complement(11066..12055)
                     /gene="rpoA"
                     /locus_tag="ACHKFK_002145"
     CDS             complement(11066..12055)
                     /gene="rpoA"
                     /locus_tag="ACHKFK_002145"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO9052482.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            complement(12081..12701)
                     /gene="rpsD"
                     /locus_tag="ACHKFK_002146"
     CDS             complement(12081..12701)
                     /gene="rpsD"
                     /locus_tag="ACHKFK_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO9052483.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            complement(12735..13124)
                     /gene="rpsK"
                     /locus_tag="ACHKFK_002147"
     CDS             complement(12735..13124)
                     /gene="rpsK"
                     /locus_tag="ACHKFK_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO9052484.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            complement(13141..13497)
                     /gene="rpsM"
                     /locus_tag="ACHKFK_002148"
     CDS             complement(13141..13497)
                     /gene="rpsM"
                     /locus_tag="ACHKFK_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO9052485.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            complement(13644..13760)
                     /gene="rpmJ"
                     /locus_tag="ACHKFK_002149"
     CDS             complement(13644..13760)
                     /gene="rpmJ"
                     /locus_tag="ACHKFK_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO9052486.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            complement(13792..15123)
                     /gene="secY"
                     /locus_tag="ACHKFK_002150"
     CDS             complement(13792..15123)
                     /gene="secY"
                     /locus_tag="ACHKFK_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO9052487.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            complement(15131..15565)
                     /gene="rplO"
                     /locus_tag="ACHKFK_002151"
     CDS             complement(15131..15565)
                     /gene="rplO"
                     /locus_tag="ACHKFK_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO9052488.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            complement(15569..15748)
                     /gene="rpmD"
                     /locus_tag="ACHKFK_002152"
     CDS             complement(15569..15748)
                     /gene="rpmD"
                     /locus_tag="ACHKFK_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO9052489.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            complement(15752..16255)
                     /gene="rpsE"
                     /locus_tag="ACHKFK_002153"
     CDS             complement(15752..16255)
                     /gene="rpsE"
                     /locus_tag="ACHKFK_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO9052490.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            complement(16270..16623)
                     /gene="rplR"
                     /locus_tag="ACHKFK_002154"
     CDS             complement(16270..16623)
                     /gene="rplR"
                     /locus_tag="ACHKFK_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO9052491.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            complement(16633..17166)
                     /gene="rplF"
                     /locus_tag="ACHKFK_002155"
     CDS             complement(16633..17166)
                     /gene="rplF"
                     /locus_tag="ACHKFK_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO9052492.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            complement(17179..17571)
                     /gene="rpsH"
                     /locus_tag="ACHKFK_002156"
     CDS             complement(17179..17571)
                     /gene="rpsH"
                     /locus_tag="ACHKFK_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO9052493.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            complement(17605..17910)
                     /gene="rpsN"
                     /locus_tag="ACHKFK_002157"
     CDS             complement(17605..17910)
                     /gene="rpsN"
                     /locus_tag="ACHKFK_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO9052494.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            complement(17925..18464)
                     /gene="rplE"
                     /locus_tag="ACHKFK_002158"
     CDS             complement(17925..18464)
                     /gene="rplE"
                     /locus_tag="ACHKFK_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO9052495.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            complement(18479..18793)
                     /gene="rplX"
                     /locus_tag="ACHKFK_002159"
     CDS             complement(18479..18793)
                     /gene="rplX"
                     /locus_tag="ACHKFK_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO9052496.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSFGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNATTGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            complement(18804..19175)
                     /gene="rplN"
                     /locus_tag="ACHKFK_002160"
     CDS             complement(18804..19175)
                     /gene="rplN"
                     /locus_tag="ACHKFK_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO9052497.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            complement(19340..19594)
                     /gene="rpsQ"
                     /locus_tag="ACHKFK_002161"
     CDS             complement(19340..19594)
                     /gene="rpsQ"
                     /locus_tag="ACHKFK_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO9052498.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            complement(19594..19785)
                     /gene="rpmC"
                     /locus_tag="ACHKFK_002162"
     CDS             complement(19594..19785)
                     /gene="rpmC"
                     /locus_tag="ACHKFK_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO9052499.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            complement(19785..20195)
                     /gene="rplP"
                     /locus_tag="ACHKFK_002163"
     CDS             complement(19785..20195)
                     /gene="rplP"
                     /locus_tag="ACHKFK_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO9052500.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            complement(20208..20909)
                     /gene="rpsC"
                     /locus_tag="ACHKFK_002164"
     CDS             complement(20208..20909)
                     /gene="rpsC"
                     /locus_tag="ACHKFK_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO9052501.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            complement(20927..21259)
                     /gene="rplV"
                     /locus_tag="ACHKFK_002165"
     CDS             complement(20927..21259)
                     /gene="rplV"
                     /locus_tag="ACHKFK_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO9052502.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            complement(21274..21552)
                     /gene="rpsS"
                     /locus_tag="ACHKFK_002166"
     CDS             complement(21274..21552)
                     /gene="rpsS"
                     /locus_tag="ACHKFK_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO9052503.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            complement(21569..22390)
                     /gene="rplB"
                     /locus_tag="ACHKFK_002167"
     CDS             complement(21569..22390)
                     /gene="rplB"
                     /locus_tag="ACHKFK_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO9052504.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            complement(22408..22710)
                     /gene="rplW"
                     /locus_tag="ACHKFK_002168"
     CDS             complement(22408..22710)
                     /gene="rplW"
                     /locus_tag="ACHKFK_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO9052505.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            complement(22707..23312)
                     /gene="rplD"
                     /locus_tag="ACHKFK_002169"
     CDS             complement(22707..23312)
                     /gene="rplD"
                     /locus_tag="ACHKFK_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO9052506.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            complement(23323..23952)
                     /gene="rplC"
                     /locus_tag="ACHKFK_002170"
     CDS             complement(23323..23952)
                     /gene="rplC"
                     /locus_tag="ACHKFK_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO9052507.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            complement(23985..24296)
                     /gene="rpsJ"
                     /locus_tag="ACHKFK_002171"
     CDS             complement(23985..24296)
                     /gene="rpsJ"
                     /locus_tag="ACHKFK_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO9052508.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            complement(24534..24953)
                     /gene="gspB"
                     /locus_tag="ACHKFK_002172"
     CDS             complement(24534..24953)
                     /gene="gspB"
                     /locus_tag="ACHKFK_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HFO9052509.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVDAETPAFKQSTQHYFFKKQPLPVVESVEEEDEPGVA
                     VENAPSSSEDEENTVEESDEKAGLRERVKNALNELER"
     gene            complement(24955..26421)
                     /gene="gspA"
                     /locus_tag="ACHKFK_002173"
     CDS             complement(24955..26421)
                     /gene="gspA"
                     /locus_tag="ACHKFK_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HFO9052510.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDRAWTRDEMAGQLLALHRTDSRCPLII
                     IENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQGIELVSTGRLTEHE
                     LKASILEGQNIDQPDLLLTARVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFTA
                     KQWRMIYRVLGDKRPRKMQLAVVMSGTILALTCGWLLLSSFTAPLPVPAWLIPVTPVV
                     KQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLVCKSGNASLQTLV
                     DQNLPWIASLKVGDKKLPVVVVRVGDATVDVLVGQQTWTLTHKWFELVWTGDYLLLWK
                     MSPEGESTITRDSSEEEILWLETMLNRALHISTESSAEWRPLLVEKIKQFQKSHHLKT
                     DGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            26601..27416
                     /gene="gspC"
                     /locus_tag="ACHKFK_002174"
     CDS             26601..27416
                     /gene="gspC"
                     /locus_tag="ACHKFK_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO9052511.1"
                     /translation="MPTLPFPFHLANHNKDAAINILIIFISIGLIIFNVNYFHTTIVK
                     NEQIINQPTNAFQSEFSLAALWRNENHAGVKDANPVAVNQETPKLSIALNGIVLTSND
                     ETSFVLINEGNEQKRYSLNEALESAPGTFIRKINKTSVVFETHGHYEKVTLHPGLPDI
                     IKQPDSENQNVLADYIIATPIRDGEQIYGLRLNPRKGLNAFTTSLLQPGDIALRINNL
                     SLTHPDEVSQALSLLLTQQSAQFTIRRNGVPRLINVSVAELTGMNGQRNEGTQ"
     gene            27400..29352
                     /gene="gspD"
                     /locus_tag="ACHKFK_002175"
     CDS             27400..29352
                     /gene="gspD"
                     /locus_tag="ACHKFK_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO9052512.1"
                     /translation="MKGLNKITCCLLAALLMPCAGHAENEQYGANFNNADIRQFVEIV
                     GQHLGKTILIDPSVQGTISVRSNDTFSQQEYYQFFLSILDLYGYSVITLDNGFLKVVR
                     SANVKTSPGMIADSSRPGVGDELVTRIVPLENVPARDLAPLLRQMMDAGSVGNVVHYE
                     PSNVLILTGRASTINKLIEVIKRVDVIGTEKQQIIHLEYASAEDLAEILNQLISESHG
                     KSQMPALLSAKIVADKRTNSLIISGPEKARQRITSLLKSLDVEESEEGNTRVYYLKYA
                     KATNLVEVLTGVSEKLKDEKGNSRKPSSTSAMDNVAITADEQTNSLVITADQSVQEKL
                     ATVIARLDIRRAQVLVEAIIVEVQDGNGLNLGVQWANKNVGAQQFTNTGLPVFNAAQG
                     VADYKKNGGITSANPAWDMFSAYNGMAAGFFNGDWGVLLTALASNNKNDILATPSIVT
                     LDNKLASFNVGQDVPVLSGSQTTSGDNVFNTVERKTVGTKLKVTPQVNEGDAVLLEIE
                     QEVSSVDSSSNSTLGPTFNTRTIQNAVLVKTGETVVLGGLLDDFSKEQVSKVPLLGDI
                     PLVGQLFRYTSTERAKRNLMVFIRPTIIRDDDVYRSLSKEKYTRYRQEQQLRIDGKSK
                     ALVGSEDLPVLNENTFNSHAPAPSSR"
     gene            29362..30843
                     /gene="gspE"
                     /locus_tag="ACHKFK_002176"
     CDS             29362..30843
                     /gene="gspE"
                     /locus_tag="ACHKFK_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO9052513.1"
                     /translation="MRIHSPYPASWALAQRIGYLYSEGEIIYLADTPFERLLDIQRQV
                     GQCQTMTSLSQADFEARLEAVFHQNTGESQQIAQDIDQSVDLLSLSEEMPANEDLLNE
                     DSAAPVIRLINAILSEAIKETASDIHIETYEKTMSIRFRIDGVLRTILQPNKKLAALL
                     ISRIKVMARLDIAEKRIPQDGRISLRIGRRNIDVRVSTLPSIYGERAVLRLLDKNSLQ
                     LSLNNLGMTAADKQDLENLIQLPHGIILVTGPTGSGKSTTLYAILSALNTPGRNILTV
                     EDPVEYELEGIGQTQVNTRVDMSFARGLRAILRQDPDVVMVGEIRDTETAQIAVQASL
                     TGHLVLSTLHTNSASGAVTRLRDMGVESFLLSSSQAGIIAQRLVRRLCPQCRQFTPVS
                     PQQAQMFKYHQLAVTTIGTPVGCPHCHQSGYQGRMAIHEMMVVTPELRAAIHENVDEQ
                     ALERLVRQQHNALIKNGLQKVIRGDTSWDEVMRVASATLENEA"
     gene            30840..32036
                     /gene="gspF"
                     /locus_tag="ACHKFK_002177"
     CDS             30840..32036
                     /gene="gspF"
                     /locus_tag="ACHKFK_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO9052514.1"
                     /translation="MNYRYRAMTLDGQKLQGIIDANDERQARLRLREEGLFLLDIRPQ
                     KSSGVKTRRPRISHSELTLFTRQLATLSAAALPLEESLAVIGQQSSNNRLADVLNQVR
                     SAILEGHPLSDALQHFPTLFDSLYRTLVKAGEKSGLLAPVLEKLADYNENRQKIRSKL
                     IQSLIYPCMLTTVAIVVVIILLTAVVPKITEQFVHMKQQLPLSTRILLGLSDTLQHTG
                     PTLLATVFIVAVGFWLWLKRGNNRHRFHAMLLRVALIGPLICAINSARYLRTLSILQS
                     SGVPLLDGMNLSTESLSNLEIRKRLANAAENVRQGNSIHLSLEQTAIFPPMMLYMVAS
                     GEKSGQLGTLMVRAADNQETLQQNRIALTLSIFEPALIITMALIVLFIVVSVLQPLLQ
                     LNSMIN"
     gene            32046..32483
                     /gene="gspG"
                     /locus_tag="ACHKFK_002178"
     CDS             32046..32483
                     /gene="gspG"
                     /locus_tag="ACHKFK_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO9052515.1"
                     /translation="MRATDKQRGFTLLEIMVVIVIIGVLASLVVPNLMGNKEKADKQK
                     AVSDIVALENALDMYKLDNHRYPTTNQGLESLVEAPTLPPLAANYNKEGYIKRLPADP
                     WGNDYVLVNPGEHGAYDLLSAGPDGEMGTEDDITNWGLSKKKK"
     gene            32491..33000
                     /gene="gspH"
                     /locus_tag="ACHKFK_002179"
     CDS             32491..33000
                     /gene="gspH"
                     /locus_tag="ACHKFK_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO9052516.1"
                     /translation="MNQQRGFTLLEMMLVLALVAITASVVLFTYGREDAASTRARETA
                     ARFTAALELAIDRATLSGQPVGIHFSDSAWRIMVPGKTPSAWRWVPLQEDAADESKND
                     WGEELSIQLQPFKPDDSNQPQVVILADGQITPFSLLMANAGTGEPLLTLVSSGSWPLD
                     QTLARDTRP"
     gene            32997..33374
                     /gene="gspI"
                     /locus_tag="ACHKFK_002180"
     CDS             32997..33374
                     /gene="gspI"
                     /locus_tag="ACHKFK_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO9052517.1"
                     /translation="MNKQSGMTLLEVLLAMSIFTAVALTLMSSMQGQRTAIERMRNET
                     LALWIADNQLQSQDSFDEENTSSSGKELINGEEWNWRSDIHSSKDGTLLEHTITVTLP
                     SGQTTSLTRYQSINNKSGQAQDD"
     gene            33367..33954
                     /gene="gspJ"
                     /locus_tag="ACHKFK_002181"
     CDS             33367..33954
                     /gene="gspJ"
                     /locus_tag="ACHKFK_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO9052518.1"
                     /translation="MINRQQGFTLLEVMAALAIFSMLSVLAFMIFSQASELHQRSQKE
                     IQQFNQLQRTITILDNDLLQLVARRNRSTDKIMVLGEEAIFTTQSRDPLAPLSEAQTL
                     LTVHWYLRNHTLYRAVRTSVDGRKDQPAQAMLEHVESFLLESNSGESQELPLSVTLHL
                     KTQQYGALQRRFALPEQLAREESPAQTQAGNNNHE"
     gene            33947..34930
                     /gene="gspK"
                     /locus_tag="ACHKFK_002182"
     CDS             33947..34930
                     /gene="gspK"
                     /locus_tag="ACHKFK_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO9052519.1"
                     /translation="MNNEQRGVALLIVLMLLALMAALAADMTISFHGQLHRTRQVNHH
                     LQRQYDIELAEKLALASLTLDVKDNDRQTTLQQYWAQPQQLQLENGNTVKWQLRDAQH
                     CFNLNALAKISDAPLASPDFPAQVFSALLINAGIDRGNTDEIVQSIADYIDADDSPRF
                     HGAEDNFYQSQTPPRHSANQMLFLTGELRQIKGITENIYQRLIPYVCVLPTSELSINL
                     NMLTENDIPLFRALFLNNITDADARVLLQKRPGEGWLTTDAFLYWAQQDFSGVKPLVA
                     QVKGHLFPYSRYFTLSTESISDEQSQGWQSHIFFNRKQQSAQIYRRTLQLY"
     gene            34945..36108
                     /gene="gspL"
                     /locus_tag="ACHKFK_002183"
     CDS             34945..36108
                     /gene="gspL"
                     /locus_tag="ACHKFK_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO9052520.1"
                     /translation="MPESLMVIRSFSTLRKHWEWMTFSADSVSSVHTLTDDLPLESLA
                     DQPGAGNVHLLIPPEGLLYRSLTLPNAKYKLTAQTLQWLAEETLPDASQNWHWTVVDK
                     QNESVEVIGIKSEKLSRYLERLHTAGLNVTRVLPDGCYLPWEVDSWTLVNQQTSWLIR
                     SAAHAFNELDEHWLQHLANQFPPENMRCYGVAPHGVAAANPLIQHPEIPSLSLYSADI
                     AFQRYDMLHGVFRKQKTVSKSGKWLARLAVSCLVLAILSFVGSRGIAFWQTLKIEDQL
                     QQQQQETWQRYFPQIKHTHNFRFYFKQQLAQQYPEAVPLLYHLQTLLLEHPELQLMEA
                     NYSQRQKSLTLKMSAKSEANIDRFCELTQSWLPMEKTEKDPVSGVWTVRNSGK"
     gene            36105..36566
                     /gene="gspM"
                     /locus_tag="ACHKFK_002184"
     CDS             36105..36566
                     /gene="gspM"
                     /locus_tag="ACHKFK_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO9052521.1"
                     /translation="MIKSWWAEKSTSEKQIVAALAVLSLGVFCWLAVIKPIDTYIEEH
                     QSHAQKIKKDIKWMQDQASTHGLLGHPALTQPIKNILLEEAKRENLTITLENGPDNTL
                     TINPVTAPLENVSRWLTTAQITYGIVIEDLQFTLAGNEEITLKHLSFREQQ"
     gene            36566..>37008
                     /locus_tag="ACHKFK_002185"
     CDS             36566..>37008
                     /locus_tag="ACHKFK_002185"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO9052522.1"
                     /translation="MTMLLPLFILVGFIAGYFVNVMAYHLSPLEDKTALTFRQVLVHF
                     WQKKYAWHDTVPLILCVAAAIACALAPFTPIVTGALFLYFCFALTLSVIDFRTQLLPD
                     KLTLPLLWLGLVFNAQSGLIDLHDAVYGAVAGYGVLWCVYWGVWLV"
BASE COUNT         9360 a         9579 c         8738 g         9331 t
ORIGIN      
        1 ttcaggattt tttctctttg ttgttacgac catcttgtga agtgtttcac gttgtcgtct
       61 caacggaggc gcattatagg gatcccaatt ttttgcacaa gtactttttt gatctttttt
      121 tctgtttgtt gctttttcac cctttttgct gcattcgcac acaaaacggt gcttttttgc
      181 atgctaaaag acttgcacaa ggccaataat gcacccaaag tcattagtaa atcatttatt
      241 gttgaggtga gtatgtctga tgttttacgc ccataccgcg atctttttcc acaaatcggt
      301 cagcgcgtaa tgatcgacga tagcagcgtc gtgattggtg acgttcgttt ggctgatgat
      361 gtggggatct ggccgctcgt tgtgattcgt ggagatgtac attatgtaca gatcggagca
      421 cgcaccaata tccaggatgg cagtatgttg catgtcactc ataaatcctc gtacaaccca
      481 gatggcaacc cattaaccat tggcgaagat gtcactgttg gtcacaaggt gatgctccac
      541 ggctgcacca ttggcaatcg agttttggtt gggatgggct caattttact tgatggcgca
      601 atagtagaag atgatgtgat gattggtgcg ggtagcctgg tcccacaaaa taaacggctg
      661 gagagcggat atctgtatct cggtagcccc gtcaaacaga tccgcccgtt aagtgatgaa
      721 gagaaggctg ggttacgcta ttccgcaaat aactacgtga aatggaagga cgaatatctg
      781 gatcaaggta accagaccca accttgatca tcttcacttt gttcctgaat taagttttcc
      841 gcttcttctt ccaggtccca gcgatgctga cgaaaactcg ctaaccactg ttctggcgta
      901 tctccagtaa agcgatacgc cagactctcg ccagagatcg cacatgtcag ttgcatacca
      961 ttcacgagag cgggaaaaca tacacatctt ttattctcgt cccactcttc cctatccgga
     1021 aactggatgg cctgattcac gctgacaatt cctgttgcaa tagtttgatg accggttcta
     1081 catcaggcag aacgccatgc caaagaagga aggcatgagc cgcctgtgcc accagcattc
     1141 ctaaaccatc agcattacgc tttgagcctc gctgctcaca ccatgccaga aaaggagttt
     1201 ttcctttctg atagaacatg tcatagcaat aaatgcctgg atgaatgagc gatgacggga
     1261 tcgccggaat atcaccactg atgccactgg atgttgcatt aataatgaga tcaaactcat
     1321 gaccttccag ttcgtccata cccaacgcct gaatactgcc agtgtgcgca aacaatttag
     1381 tcaactcttc tgcgcgagat accgtccgat tagtgattgc caccgcacag tccagggaaa
     1441 ggagtggcag tagtacgccg cgagatgctc caccagcgcc aatgagcaga atacgtaaac
     1501 caggacggat aaaagacaga cgttccagat cgcttaacaa acctatacca tcggtattgt
     1561 cacccagcag gcgtccatct tctaaccgct tgagggtatt aacagcacca gccaacgctg
     1621 cccgttcagt aagctcatcc gctctggcaa aagcctcttc tttaaaaggt accgtcacat
     1681 tcgcaccttt accaccagca ctaaagaaag cgttcagtgt gttgatgaaa tcattgatgg
     1741 gtgccaacac gcgtccatag ggatgttcaa tattcagttg ctgagcaaat tgctgatgaa
     1801 tgaatggcga tttgctgtgg gctatcggat taccaaaaac agcataggtt tccattatgt
     1861 tacccctgtc gaaacagttc acccgtcagg gcatcgcgga tttctgaagg atttaaacgc
     1921 ccccccgttt caccaggcac aaccgggaac gccgcgccaa attgtgcgcg aacttcgtct
     1981 actgttcgac aaggtggcaa tccactcaag ttggcactgg tagaaaccag cggtttacca
     2041 taagcttggc acaaagcaac caccaacgga tggtcggtga ctcgtacagc aagcgaatca
     2101 aagcggcccg tcaaccagcg cggtgttgtc gcaggcgcgg gaaaaacaaa ggtgacagga
     2161 cctggccagc gagaaaatat agtttcacgc tgcgcgtcag tcagcatggt gtcatcaata
     2221 tagggtttaa gctgctcgta atttgcggcg attagaatca gccccttatc aaccggacgc
     2281 tgttttaact ccaacagtcg catcactgct gtttcgctat caggatcgca cccgacaccg
     2341 aaaacggctt ccgttggata ggcgatgaca cgttcttcat tgaggacatc tatcgcagct
     2401 gcgatagcgt ctccttgcag gttattattc acgttattat tccgccgaaa tcggctttcc
     2461 acattgttta ctggcacaaa agtgttttac accctgcgcg gttttctttt cgatgagtag
     2521 cggataatgg cactcagggc attctccagc tatgggtttg aagttaatgg caaattgaca
     2581 ctccgggtag cgatcacagg agtgaaatgt tttgccataa cgggagcggc gctgaaccag
     2641 atggcccgtc cggcattggg ggcatgtaat tgctgtttcg tccggtttat cgataagttc
     2701 ggtatgttcg cattcagggt agttactgca accaataaac ataccaaagc gtccctggcg
     2761 taataccaga tttgcgccac atgcagggca aacctgcccc tccagaactt tgacaatatg
     2821 tccatccgct gaagatttca gaggacggac gtagtcacac gccggatact gtgagcatcc
     2881 aagaaacgga ccgtgtttcc cggatcgaat aactagttca gccccgcact ttgggcagga
     2941 ctcattatta cgcaccgtga acagtgctga tttcgccata actacttatg ctgaattaaa
     3001 gaaatgatta atgcagcata ccttcattca cttcaaagag taattcttcc atttgctggt
     3061 acgcattttc gcagcccgga atattgaaca acaccatcag gattacccat ttcaggtctt
     3121 ccaggtcgaa ctcagcggta tccagcgcga gcactcgctc tatcaccatt tcacgggttt
     3181 caaggttaag cacctgaatt tgctcaagga aaagcagaaa accacgacag ctggcatcca
     3241 gtctttcaca ctcttcaggg gtataaatac gcatggagag tggatcagag gccagttgca
     3301 tcggttctgc cagtccttcc tgataatccg caagcttttc cagccaaagc agggcattgt
     3361 agatatcttc tcgatcaaat cctgcgtcgg taagatcctg ttcaagtttg tcttgatcca
     3421 cacgcaactc agcttctgtg tgaatatagg tttcaaacaa atacattagt acgtcgaaca
     3481 tggcatgccc tcctcaatcg gacatagccg ccgggtacag ctgcgatcca tcctgctaac
     3541 tccagttcga gtagttgagt aactacctct ggcacaggtt ggccggcacg ttcagcgacg
     3601 acgtcaacag gtgttacctc atctcctacg ttagccagga gctcaggaaa tggcaatgcc
     3661 acgtcttcct gatctggtga ataaaatgaa ttttcagggg cgtctggcaa ccagtgcaat
     3721 ccaaattgca agttttccag aatttcttcc ggttccgtca caagaatcgc accttgtttt
     3781 attaaccagt gaggcccttc gcttcccgga ttccctattg gacctggcaa agcaaaaact
     3841 tcacgcccct gctcaagcgc acaacgtgct gtcaccagcg aaccactgcg caaagccgct
     3901 tccaccacca gtacaccttt acttagacca ctgataatgc gatttcttcg tgggaaattg
     3961 taagcaaggg gtggaacatc gaggggaaat tccgagacga gagcaccacc ttgctcaagt
     4021 agactggcag ccagtggagc atggcggcgg ggatgaatgg tattaagtcc attccccaat
     4081 acagcaatgc taacgccatt tacctgtaag gccgctttat gcgccacacc atcgattcca
     4141 cgcgccagtc cactcgtaat tgtcactcca cgcgtcgcca gagtttcgca aaataatcgt
     4201 ccccatcgct cgccatacca tgaatgcgcc cgactcccca ctacggcaag ctgaaatgaa
     4261 tgcaacgcgt gcagttctcc ttcaacaaac agtgcgccgg ggtaatctgt cgtcgccaga
     4321 agttgaggag gataaaattc gctgtccgca gggattaaat gatggttggg ttgctccaac
     4381 caacaaagtg agctttcgat actctttcgc ggaaatgaaa gaaagcgttg tgcctgccgc
     4441 aatgtaagcc ctgtttgctg caatacaacc gcatcaatat gcgactgttt tgccagccag
     4501 tgagctatac ggaccatatc atcgccatac aagctgctga tgctaattaa acgcagccaa
     4561 atttctgtat cgaccatcct tatctccctg ccataagcag ccttagcaat ctttgcgatt
     4621 ggtcagtgat gctgtcaatc agagggggat ttgtctagaa tagaagaaat aatctttcta
     4681 actcctgaac acatctctgg agatttatgt cagttttgca agtgttacat attccggacg
     4741 agcggcttcg caaagttgct aaaccggtag aagaagtgaa tgcagaaatt cagcgtatcg
     4801 tcgatgatat gttcgagacg atgtacgcag aagaaggtat tggcctggcg gcaacccagg
     4861 ttgatatcca tcaacgtatc attgtaattg atgtttcgga aaaccgtgac gaacggctgg
     4921 tgttaatcaa tccggagctt ttagaaaaaa gcggcgaaac aggcattgaa gaaggttgcc
     4981 tgtcgatccc tgaacaacgt gctttagtgc cacgcgcaga gaaagttaaa attcgcgccc
     5041 tggaccgcga cggtaaacca tttgaactgg aagcagacgg tctgttagcc atctgtattc
     5101 agcatgagat ggatcacctg gtcggcaaac tgtttatgga ttatctgtca ccgctgaaac
     5161 aacaacgtat tcgtcagaaa gttgaaaaac tggatcgtct gaaagcccgg gcttaaggat
     5221 aagaattaac gtgtcagaat cactacgtat tatttttgcg ggtacacctg actttgcagc
     5281 gcgtcatctc gacgcgctgt tgtcttctgg tcataacatc gttggcgtgt ttacccagcc
     5341 agaccgaccg gcaggacgcg gtaaaaaact gatgcccagc ccggttaaag ttctggctga
     5401 ggacaaaggt ctgcccgttt ttcaacctgt ttccctgcgt ccacaagaaa accagcaact
     5461 ggtcgccgat ctgcaggctg atgttatggt cgtcgtcgcc tatggtttaa ttctgcctaa
     5521 agctgtgctg gagatgccgc gtcttggctg tatcaacgtt catggttcac tgctgccacg
     5581 ctggcgcggt gctgcaccaa tccaacgctc actatgggcg ggtgatgcag aaactggtgt
     5641 gaccattatg caaatggatg ttggtttaga caccggtgac atgctttata agctctcctg
     5701 cccgattact gcagaagata ccagtggtac gctgtacgac aaactggcag agcttggccc
     5761 acaagggctt attactacgt tgaaacaact ggcagacggc acggcgaaac cagaagttca
     5821 ggacgaaact cttgtcactt acgccgaaaa gttgagtaaa gaagaagcgc gtattgactg
     5881 gtcactttcg gcagcacagc ttgaacgttg cattcgcgct ttcaatccat ggccaatgag
     5941 ctggctggaa attgaagggc agccggttaa agtctggaaa gcatctgtca tcgatacgac
     6001 aacaaaggct gcgccaggaa caatccttga agccaataaa caaggcattc aggtagcgac
     6061 aggtgatggc atcctgaatc tgctctcaat gcaacctgca ggtaagaaag cgatgagcgt
     6121 gcaagacctc ctgaattctc gtcgggaatg gtttgtaccg ggcaaccgtc tggcctgata
     6181 gtccactctt ctaagcccgg tcttaccggg catctttata tttatactta tgaaaaaaca
     6241 acgtaattta cgtagcatgg cggcccaggc cattgaacaa gtcgttgagc aagggcaatc
     6301 actgagcaac attctgccac cgctccagca aaaagtttcc gataaagata aagctcttct
     6361 ccaggagtta tgctttggcg tactgcgtac gctctcacag ttagactggc tgattaataa
     6421 gctaatggcc cgtccgatga caggcaaaca gcgtactgtt cattacctga ttatggttgg
     6481 tttgtatcaa ctgctttata cccgcattcc acctcatgcc gcgctggctg aaacggttga
     6541 aggcgcagtt gcaattaagc gcccgcaact taagggattg attaacggtg tattacgtca
     6601 gttccagcgt cagcaagatg agttattagc cgagtttaat gccagtgatg cacgttatct
     6661 gcatccttcc tggttgctga agcgtctgca aaaggcgtat cccgagcagt ggcaatccat
     6721 cgtcgaagcc aataaccagc gtccgccaat gtggctgcgc gttaatcgta cgcatcattc
     6781 ccgcgacagc tggcttgcat tgctggatga agcaggaatg aaaggtttcc cgcatgcgga
     6841 ttaccctgat gctgtacagc tggaaacacc tgcacctgtt catgcgctac ccggttttga
     6901 agaaggatgg gttaccgttc aggatgcttc agcacaaggt tgcatgacct ggcttgctcc
     6961 acaaaatggt gaacacattc tggatctttg cgccgcccca ggcggtaaaa caacgcatat
     7021 tcttgaggtt gctccagagg cgcaagttct ggcggtcgat attgatgagc agcgcctctc
     7081 tcgcgtttac gacaatttaa aacgccttgg tatgaaggca accgtaaaac aaggtgatgg
     7141 ccgttaccct tcccaatggt gtggtgagca acagttcgat cgcattttat tagatgcacc
     7201 ttgttcggca actggtgtaa ttcgtcgcca tccggatatt aaatggttac gccgcgatcg
     7261 cgatatccca gaactcgcgc agttgcagtc tgaaattctc gacgccatct ggtcgcattt
     7321 aaaatccgga ggaaccctgg tctatgccac ctgttcggtg ttaccggaag agaatagcct
     7381 gcagattaaa gcctttttgc aacgtaccgc tgatgccgaa ctttgcgaaa caggaacacc
     7441 agagcaaccg ggtaaacaaa atttgcccgg tgccgaagag ggcgacggct tcttttacgc
     7501 taagctaatc aaaaagtgat gagataacgg gtcgcgactg atgaaaatta tcattctggg
     7561 tgccggccag gttggcggca cactggcgga aaacctggtt ggcgagaaca acgatattac
     7621 tgttgtcgat accaacggtg agcgtctgcg gaccttacag gataaatttg acctgcgagt
     7681 cgtgcagggg catggctctc atccacgcgt attgcgggag gcaggtgccg acgacgccga
     7741 tatgctggtt gctgtaacca gttcagatga aaccaatatg gttgcctgcc aggtagccta
     7801 ctcacttttc aacaccccta atcgcatcgc tcgtatccgc tcaccagact acgtgcgcga
     7861 tgccgataag ctatttcatt cagatgctgt tccgattgat catctgatcg caccagagca
     7921 gttggttatc gataatattt accgactgat tgagtatccc ggcgcattgc aggtggtgaa
     7981 cttcgctgag ggtaaagtca gcctggctgt ggttaaagcc tattatggcg gcccgctgat
     8041 tggtaatgca ctttcgacca tgcgcgaaca tatgccacat atcgatactc gtgtggcagc
     8101 aattttccgc cacgatcgcc ccattcgtcc gcaaggttcg accattgttg aagctggtga
     8161 tgaagtgttc tttattgccg cttcacagca tatccgcgcg gtgatgagtg aattacagcg
     8221 actggaaaaa ccgtataagc ggatcatgct ggttggtggc ggtaatatcg gtgcagggct
     8281 ggcgcgtcgt ctggaaaaag attacagcgt taaactcatc gaacgtaatc agcagcgcgc
     8341 tgccgaactg gcggaaaagt tgcagaatac gatcgtcttt tttggtgatg cgtcggatca
     8401 agaactgctg gccgaagaac atatcgatca agttgatctg tttattgccg tcaccaacga
     8461 tgacgaggcc aatatcatgt ccgccatgct tgccaaacgt atgggtgcga aaaaggtgat
     8521 ggtattgatc caacgtcgcg cttatgtgga tctggttcag gggagcgtga tcgatattgc
     8581 gatttcacca caacaagcaa ctatttctgc gttgcttagc catgtgcgaa aagcagatat
     8641 tgttggtgtt tcctcattac gccgcggcgt agcagaagct attgaagccg ttgctcacgg
     8701 tgatgaaagc acctcacgcg ttgtcggcag agtcattgac gaaatcaagc taccgccagg
     8761 aacgattatt ggagcggtgg tacgtggaaa cgatgtgatg attgccaatg acaatctgcg
     8821 cattgagcaa ggcgatcacg taattatgtt cctcacagat aaaaagttta ttaccgacgt
     8881 cgaaagactc ttccagccaa gtcctttctt cttgtaatta ataaggcgtc taatgacgcc
     8941 ttattatttc cctttgatta ttaaggatta attaaatcca ttcctggcag gaaaatggct
     9001 taacatttgt tagacttatg gttgtcggct tcatagggag aataacatga gcattattaa
     9061 agaatttcgc gaatttgcga tgcgcgggaa cgtggtggat ttggcagtgg gtgtcattat
     9121 cggagcggca ttcgggaaga ttgtatcttc actggttgcc gatatcatca tgccacctct
     9181 gggcttatta attggcggga tcgactttaa acagtttgct gtcacgctac gcgaggccca
     9241 aggggatatc cctgctgttg tgatgcatta cggtgtcttc attcaaaacg tctttgattt
     9301 tctgatagtg gcctttgcca tctttatggc gattaagcta atcaacaaac tgaatcggaa
     9361 aaaagaagaa ccagcagcag caacacctgc accaactaaa gaagaagtat tactgacaga
     9421 aattcgtgat ttgctgaaag agcagaataa ccgttcttaa caagcgtctg aaagcagaag
     9481 gccagtggta aaaaagtgat ttactttctt gccactggcc tcccagttcc cccgattgcc
     9541 atgttttcct tttcgcatgt aactgccttt ccctttctta tttttctcta ctcgctgtcg
     9601 gaataaggga tcatgtagta atgcttctat cgcattatcc tttatctgcc ctttagtatg
     9661 ctgatatcgg ctcataaaaa ctccagttgg ttatttaacg gcgcgagtgt aatcctgcca
     9721 gtgcaaaaaa tcaacagcca ctcttaacgc cactcgcccc ttgttcaaga gcttcaagaa
     9781 tcgagcaata aacactgcta tgggcggtcc cacaacaggt atcattaagg cgttgcaagg
     9841 aacgctgcat actctgcaac tcggctatac gagcttcgac ttcctgcaat cgttcctgca
     9901 caatgccttt tgactcctga caggtatggt gttccggatc gatacggatc gacagcaact
     9961 cgcggatcga ctccagactg aaacctagtt gtctggcatg gcggataaat ttcaatcgct
    10021 ggagatcgct tttggtatac aggcgaaacc caccttcggt gcgtacttca tgctccatca
    10081 tctgctgctt ttcgtaataa cgaatcgtgt cgggtgttac ttccgccatt tttgccagct
    10141 caccaatgcg atacatacat actccactag ttatcgttga ttttgttcaa caacttgtca
    10201 gcataatcgc cgcgtaaaaa atcggtactc aatccagctt gtcgcagctt caattgcagt
    10261 aatgacaatc gacgactaac ctcttgatat tgcggatcat catgacggat acctttgaga
    10321 atatccagaa gctgtattgc ttccctccta ttctcaagtt ctggcggtaa gcaaccagca
    10381 ttcttcagca agcgataccc cgcacgtaat tccggtggca catgagaatc atcatccagt
    10441 atcaatggtt cgccgctacc taccaggtta tcaaactcac ctttcgcttg cgcttctgct
    10501 atatgacgct ctgcccactg gtcaagtaac cacataaata ctccagggga tgaacaaaaa
    10561 gagtacagct attgtagata agtggggatg ctacgggtat aaaaaaaccc gccggggcgg
    10621 gtttttttac gttgcttcag attactctgc agcagcttct gctttctctg aacgatcaac
    10681 cagctcgatg taagccatcg gcgcattgtc gcctgcacgg aagccacact tcagaatacg
    10741 agtgtaacca ccggcacggc tcgcgaaacg cgggcccagt tcgttaaaca gttttgccac
    10801 gatctcgtta tcacgagtac gggcgaatgc cagacgacga ttagcaacgc tatcagtctt
    10861 ggcaagagta atcagcggct caactacgcg gcgcagctct ttcgctttag gcagagtcgt
    10921 cttgatgatt tcatgacgaa ccagtgaacc tgccatattg cggaacatag cctggcgatg
    10981 gctgctgttg cggttcagtt gacgaccact cttacgatgg cgcatgacct tatccttctc
    11041 agtaaaacct taacctgtga tccggttact cgtcagcgat gcttgccggt ggccagtttt
    11101 ccaggcgcat gcccagagac agtccacggg aagccagcac gtctttaatc tcagtaagag
    11161 attttttacc aaggttaggc gttttaagga gctcaacctc ggtacgctgt accagatcac
    11221 cgatatagtg gatagcttct gctttaaggc agttagcaga gcggacagtc aattccagat
    11281 cgtcaacagg gcgcagcagg atcggatcga actctggttt ctcttctttc acttcaggct
    11341 gacgtacatc acgtaagtca acgaaagctt ccagttgttc agccagaatg gttgccgcac
    11401 gacgaatcgc ctcttcagga tcgattgtgc cgttggtttc catttcgatg accagcttgt
    11461 ccaggtcggt acgctgttct acacgcgctg cttcaacatt gtaggcaata cgctccacag
    11521 ggctgtagca tgcgtcgacc agcagacggc cgattgggcg ctcatcttct tccgaatgaa
    11581 ttcgggtaga agccggcaca taaccacgac cgcgctgaac tttgatacgc atgctaatag
    11641 acgcgttctc atcggtcagg tggcagatca cgtgctgcgg cttgacgatt tcgacatcac
    11701 cgtcgtgggt gatatcggct gcagtcacag ggccaatgcc agatttattc aaggtaagaa
    11761 taacttcatc tttgccctga actctcaccg ccagcccttt caggttgagc aggatttcca
    11821 ggatatcttc ctgaacgcct tctttggtgc tgtactcatg tagtacacca tcaatctcaa
    11881 cctcggtcac cgcgcaaccc ggcatcgatg agagcagaat acggcgcagt gcgttaccca
    11941 gagtatggcc aaagccacgc tctaaaggct caagggtcac cttggcgtgc gtcgaactca
    12001 cttgctcgat atcaaccagg cgcggtttta gaaactctgt cacagaaccc tgcattgtgt
    12061 cctctctttg gtactaagct ttacttggag taaagctcga cgatcaggtg ttcgttaatg
    12121 tccgcagaca gatcagaacg ctccggctta cgcttaaacg taccttccat cttgccagca
    12181 tcaacttcca gccaggttgg cttttcacgc tgctcagcca gctccagagc ggctttcacg
    12241 cgagactgct tcttcgcttt ctcacgaatg cttacaacgt cattcggact aacctgataa
    12301 gaagcgatgt taacaacacg accgtttacc ataattgctt tatggctaac cagctgacgt
    12361 gcttctgcac gagtggcacc gaagcccata cggtatacaa cgttgtccag acgaccttcc
    12421 agcagagcca acaggttttc accggtgttg cctttcagac gtgctgcttc tttgtagtag
    12481 ttacggaact gacgctccag cacaccatag atacggcgaa ctttttgctt ttcacgcaac
    12541 tgcacaccat agtcagacag acgcggttta cgcgcaccgt gctggccagg agcttgttca
    12601 attttacact tggtatcgat cgcgcgaacg ccagacttaa ggaataagtc ggtgccctca
    12661 cgacggctca gcttgagctt aggacccaaa tatcttgcca ttttctttct ccaacaaacc
    12721 tggaaaacga ggcgttatac gcgacgtttt ttcggcggac gacaaccgtt atgagggatc
    12781 ggagtcacat cagtaatgtt agtgatgcgg aaacctgcgg cgttcagagc acgaatagta
    12841 gattcgcggc ctggacccgg acctttaacc ataacttcca gattcttgat gccgtattct
    12901 ttcacggcgt cagcgcaacg ctctgctgca acctgtgctg caaacggagt ggatttgcga
    12961 gaaccacgga aaccggaacc accggctgtt gcccaaccca acgcgttacc ctgacgatca
    13021 gtgatagtca cgatggtgtt gttgaaagaa gcatggatat gagccacgcc gtcagagact
    13081 tgttttctta cacgtttacg tgcacgaatt ggtgcctttg ccattattca atcaccccga
    13141 ttatttcttg atcggtttgc gcggaccctt acgggtacgt gcgttggtct tggtacgctg
    13201 accgcgaacc gggagaccac gacgatgacg caaaccgcga tagcaaccaa gatccatcag
    13261 gcgcttgatg ctcatgctga tttcacggcg cagatcacct tcaacgacaa atttggcaac
    13321 ttcgtcacgc agcgtgtcga tttgtccttc agacagctca ctgatcttaa catcttcagc
    13381 gatacccgct gcagccagga tggctttaga acgggtcttg ccgacgccat aaatcgaagt
    13441 taatgcgatt acggcatgct tatgatcagg aatgttaatg cctgctatac gggccactat
    13501 gcactcctac tatttaatat gtacgttcca tgctgaaaag cccgttttca ggatactcaa
    13561 atggaaacgc acagacatac aaaagattgg ctggctaatc tagccagctc aacccaactt
    13621 tgcaagaaaa atatgcgaaa aaatcagcct tggcgctgtt tatgcttcgg ctcggcactg
    13681 caaatcacac ggatgacacc atcacgctta acgattttgc agttacggca taatttcttg
    13741 acggaagcac gaactttcat ttttactctc cgtaacttct cgggcgacca attatcggcc
    13801 gtagcctttc aggttcgcct tcttcaatgc agactcatac tgactggaca tcatcagagt
    13861 ttgcacttga gccataaagt ccataatcac gacaacaacg ataagcagtg aggtcccacc
    13921 gaagtagaac ggtactttca ttgcatcacg catgaactcc gggatcaggc agataaaggt
    13981 aatgtacagc gcaccaacca aggtcaggcg ggtcattact ttatcgatat acttcgccgt
    14041 ttgctctccc ggacgaattc ctggtacaaa tgcaccggac ttcttcaggt tatctgctgt
    14101 ttcacgcggg ttgaaaacca acgccgtgta gaagaaacag aagaagatga ttgcagacgc
    14161 atagagtaac acataaagcg gttgcccagg ctgcaaatac agcgaaattg ttgtcagcca
    14221 gttccaacca gtaccgcccc cgaaccatga cgcgatggtc gccgggaaca gaataatact
    14281 ggaagcgaag attgccggga ttacccccgc catattcact ttcagcggta aatgtgtgct
    14341 ctgtgcagca tagacacgac gaccttgctg acgtttcgcg tagtttacca caatgcggcg
    14401 ttgaccacgc tcaacaaata caacaaagaa cgtcactgca aatactaata ctgcaaccaa
    14461 cagcaacacg aggaagtgca ggtcgccttg acgcgcttgc tcgatagtat gggcaatggc
    14521 tggcgggagt cccgcgacaa taccggcgaa gataatgatt gaaataccgt tgccgatacc
    14581 tcgttcagtg atctgttcgc ccaaccacat caggaacatg gttcctgtga ccagacttac
    14641 aacagcggtg aagtagaatg caaagcccgg gttaatcacc aggccttgca taccaggcat
    14701 attcggcaga ccagtagcaa taccgatcga ctggaatatt gccagcacca gagtaccgta
    14761 gcgggtgtac tggctgatct tacgacgacc agactcccct tctttcttaa tttctgccaa
    14821 cgttggatga accaccgtca gtagctggat aatgatcgac gccgaaatat acggcatgat
    14881 ccccagagca aagatagaag cacggctgag agcaccacca gagaacatgt taaacatctc
    14941 aatgatggtg cctcgctgtt gctcaagcag tttggcaagt acagcggcat caataccagg
    15001 gatcggaata aaagagccaa tacggaacac aatcagcgca ccgataacaa acagcagtct
    15061 gcgtttcagc tcgcctaagc cacctttggc actttgaaaa tctaatcccg gttgtttagc
    15121 catctgctac ttattcctcg attttaccgc cagcagcttc gatagcagca cgagcgcctt
    15181 tagtaacacg caggccacga acagttaccg gagtcgttac ttcgccagcc aggatcactt
    15241 tcgcgaactc gatctggata ccgataatgt tagccgcttt cagcgtgttc aggtctacaa
    15301 caccgccttc tactttagcc aggtcagaca gacgaacttc ggctgtaatc gctgctttac
    15361 gagaagtgaa gccgaatttc ggcagacgac ggtacagagg catctgacca ccctcgaaac
    15421 cgcgacgtac gccaccgcca gaacgagatt tctgaccttt gtgaccacga ccaccggttt
    15481 taccgaggcc agaaccgata ccacgaccca ggcgtttacc cgcctttttg gagccttcgg
    15541 ccggagacag agtatttaaa cgcatctctt actcctcaac tttaaccatg aaggaaaccg
    15601 cgttgatcat accgcgaata gcaggagtat cctcgcgctc tacggtgtga ccaatacgac
    15661 gcagacccag gccaagcagc gttgccttgt gtttcggcag acgaccgatt gcactgcggg
    15721 tttgagtaat tttaatagtc tttgccatgg tttatttccc cagaatttct tcaacggatt
    15781 taccacgctt ggcagcgacc atttctggag aattcatatt ttccaggcca tcgatagttg
    15841 cacgaaccac gttaatcggg ttggtggaac catacgcttt agccagaacg ttatgaaccc
    15901 cagcgacttc cagaacggca cgcattgcac caccggcgat gataccggta ccttcggaag
    15961 ccggctgcat gaatacgcga gaacccgtgt gaacaccttt aacagggtgt tgcagagtgc
    16021 cgttattcag cgcgacgtta atcatattgc gacgggcttt ttccatcgct ttctggatcg
    16081 ctgctggaac ttcacgcgct ttaccgtaac caaaaccaac gcgaccgtta ccatcgccaa
    16141 ctacagtcag agctgtgaag gagaaaatac gaccaccttt aacggtttta gatacgcggt
    16201 ttaccgcgat cagcttttcc tgcagttcgc cagcttgttt ttcgatgtga gccatcttac
    16261 acctctacct tagaactgaa ggccagcttc acgggcagca tctgccagtg cctggacacg
    16321 accatgatat tggaacccgg aacggtcaaa ggatacatct ttgatgcctt tttccagagc
    16381 gcgttcagcg acagctttac ccacagctgc agccgcgtct ttgttaccgg tgtacttcag
    16441 ttgttcagcg atagcttttt ctacagtaga agcagctacc agaacttcag aaccgttcgg
    16501 tgcaattacc tgtgcgtaaa tgtgacgcgg ggtacgatgt accaccaggc gagttgcgcc
    16561 cagctcctgg agcttgcggc gtgcgcgggt cgcacgacgg atacgagcag atttcttatc
    16621 catagtgtta ccttacttct tcttagcctc tttggtacgc acgacttcgt cggcgtaacg
    16681 aacacccttg cctttgtaag gctcaggacg acggtaggcg cgcagatccg ctgcaacctg
    16741 gccgatcacc tgcttatcag cgcctttcag cacgatttca gtctgagtcg gacattcagc
    16801 agtgataccc gcaggcagct gatggtcaac aggatgagag aaacccagag acaggttaat
    16861 cacattgcct ttaaccgctg cacggtaacc tacaccaacc agctgcagct tcttagtgaa
    16921 gccttcggta acaccgataa ccattgagtt cagcagggca cgcgcggtac cagcctgtgc
    16981 ccaaccgtct gcgtaaccat cacgcggacc gaaggtcagg gtattatctg catgtttaac
    17041 ttcaacagca tcgttgagag tacgagtcag ctcgccgttt ttacctttga tcgtaataac
    17101 ctgaccgttg atttttacgt caacgccggc aggaacaacg accggtgctt tagcaacacg
    17161 agacattttt tcctccgatt aggctacgta gcagataatt tcgccaccaa gaccagcctg
    17221 gcgcgctgca cgatcagtca taacaccttt agaggtagaa acaactgcga tacccagacc
    17281 cgccataact ttcggcagct catctttacg tttatagatg cgcagacctg ggcggctgac
    17341 acgctgaatg ctttctacaa cagctttgcc ctggaaatac ttcagagtaa gttccagttc
    17401 aggcttggtg tcgccttcaa ctttaaaatc ttcaataaaa ccttcttcct tcagcacgtt
    17461 ggcgattgcc actttcagct tggaggaagg catggtgacc gcagctttgt tcgcggcctg
    17521 accgttacgg atacgggtca gcatatccgc gatcggatct tgcatgctca tctgtcttta
    17581 ctcccgtgat tcaattggtg acaattacca gctagccttt ttcagacccg ggatttcacc
    17641 gcgcatagcg gcttcacgga ccttaatacg gctcaacccg aacttccgca ggaaaccatg
    17701 cggacgacct gtttgacggc agcggttacg ctgacgagac gggctggaat cacgcggcag
    17761 agtctgcagc ttgagaacag cgttccaacg atcttcgtcg gaagcgttca catcagagat
    17821 gatcgctttc agttcagcgc gtttcgcgaa gtatttatca gctaaagcta cgcgttttac
    17881 ttcgcgtgct ttcattgatt gcttagccat ttagtaaccc taccttactt gcggaacggg
    17941 aagtcaaagg cagccagcag agcgcggcct tcttcgtcag atttcgcagt agtggtaatg
    18001 gtaatatcca aaccacgaac gcggtcgact ttatcgtagt cgatttctgg gaagatgatc
    18061 tgctcacgga cacccatgct gtagttacca cgaccgtcga aagacttagc ggacaggcca
    18121 cggaagtcac ggatacgagg tacagcaata gtgatcaggc gctcaaagaa ctcccacatg
    18181 cgttcgccac gcagagttac tttacagccg atcggatagc cctgacggat tttgaagcct
    18241 gcaacagatt tgcgtgcttt ggtgatcagc ggtttttgac cggagattgc tgccaggtct
    18301 gctgctgcgt tatccagcag ttttttgtca gcgatcgctt caccaacacc catgttcagg
    18361 gtgatcttct cgacccgagg gacttgcatg acagaattgt agttaaactc agtcatgagt
    18421 tttttaacta cttcgtcttt gtagtaatca tgcagtttcg ccatcgtact actccaaatt
    18481 acttgatagt ttcgctgtta gacttgaaga aacggacttt tttaccgtct tcgaatctaa
    18541 agcctacacg gtcagccttg ccggttgtcg cattgaagat tgctacgttg gaaacctgaa
    18601 tagcggcttc tttttcaacg atgccacccg gttggttcag ggccggaacc ggcttctgat
    18661 gtttcttaac caggttgata ccttcaacaa tgaccttgcc gaaagacagg acattcttaa
    18721 ctttaccgcg tttaccttta tctttaccgg ttaacacgat aacttcgtca tcacgacgga
    18781 ttttcgctgc catgattcgc tccttagagt acttctggtg ccagagagat aattttcatg
    18841 aacttctcac tacgaagctc acgagttacc ggcccaaaaa tacgcgtacc gataggctgc
    18901 tcgctgttgt tgttcagaag aacacaagca ttaccatcga agcgaatgac agaaccgtcc
    18961 gggcgacgaa cacccttctt ggtgcgcacc actaccgcct tcagcacatc accttttttg
    19021 accttaccac gcggaattgc ttctttgatg gtgatcttga tgatgtcgcc tacgcctgcg
    19081 tagcgacggt gcgagccacc cagaaccttg atacacatta cgcgacgtgc accggagttg
    19141 tcggcgacgt tcagcatagt ctgttcttgg atcattttag tgctccgcta atgtcaacta
    19201 ctactgagac ccgaaaatca ggtcgttaaa aatccccata tcgagggcgc ggcattataa
    19261 caccgcttca aggatatggg tagaaaaaat aaacggctca tttctgagcc gtttattcgt
    19321 atcgagagag tgtactgtat tacagaaccg ctttctctac aacgcgaacc agcgtccagg
    19381 atttagtctt ggacagcgga cggcattcgc ggatttcaac cacgtcaccg ataccgcatt
    19441 cgttgttctc gtcatgtacg tgcagtttgg tcgtacgctt gatgaattta ccgtagatcg
    19501 ggtgtttcac aaaacgttcg atagcaacaa caatggattt ctccattttg tcgctaacaa
    19561 cgcgaccttg cagagtacgg attttatcgg tcattacgca cccgccttct cgttcagtaa
    19621 agtcttaacg cgtgcgacat cgcgacgcac ttgcttcaac aggtgagact gttgcagctg
    19681 gccacttgca gcctgcatac gcaggttgaa ctgctcacgc agcaggttca gcagctcggt
    19741 gttcagctct tcaacgctct tctcacgcag ctcttttgct ttcattacat caccgtctta
    19801 gttacaaagg tggttttaat cggcagtttc gctgctgcca gcttgaatgc ttcacgggcc
    19861 agctcttccg gaacaccgtc catttcatac aggactttac ccggctgaat caaggcaacc
    19921 caatactcca cgttaccttt acctttaccc atacgcactg ccagcggctt ttcagtgatc
    19981 ggtttgtccg ggaacacacg gatccagatc ttaccttgac gcttaactgc acgggtcata
    20041 gcacgacgtg ctgcttcgat ctgacgggca gtcagacgac cacggccaac agctttcaga
    20101 ccgaagctgc cgaagctaac atccgtaccc tgcgccagac cgcggttacg gcctttgtgc
    20161 attttacgga attttgtacg ctttggttgt aacatcagcg acgctcctta tttacggcct
    20221 ttacgctgct gctttttagg ctgagcagcc ggtttttccg gttgttcaac agcagccata
    20281 ccacccagga tctcgccttt gaagatccac actttaacgc cgattacacc gtaagtggtg
    20341 tgcgcttcag aggtgttgta gtcgatgtca gcacgcagag tgtgcagcgg tacgcgacct
    20401 tcgcggtacc attcggtacg tgcgatttcc gcgccgccca gacggccgct aacttcaact
    20461 ttaatacctt tagcgcccag acgcattgcg ttctgtacag cacgcttcat agcacgacgg
    20521 aacataacgc gacgttccag ctgagaagtg atgctgtcag caaccagttt tgcgtccagt
    20581 tcaggcttac gaacttcggc gatgttgatc tgtgcaggaa cgccagcgat gtccgctacg
    20641 accttacgca gtttttctac gtcttcacct tttttaccga taacgatacc cgggcgagca
    20701 gtgtgaatgg ttacacggat gctcttagcc ggacgctcga taacgatacg agatacggac
    20761 gctttagcca gttccttagt caggtactga cgtactttaa aatcgctgtc caggttgtca
    20821 gcgaattctt tggtgttcgc aaaccaggta gagttccatg gttttacaat acccaggcga
    20881 ataccattag gatgtacttt ctgacccatt gctagtctcc agagtctcag cgatcggaca
    20941 caaccacagt gatgtggctg gtgcgcttca ggatgcgatc tgcacgacct tttgcacgcg
    21001 gcataatgcg cttcatgctc gggccttcgt ctacgaaaat tttcgtaact ttcagatcgt
    21061 caatgtcagc gccatcgttg tgttcagcgt tagcaatggc agattccaga actttcttga
    21121 ccagtacagc cgctttcttg ttggtgtagg tcaaaatatc cagagcctgc gacactttct
    21181 taccgcgaat caggtcagca acaaggcgaa ccttctgagc agaagaacga gcatggcgat
    21241 gtttagcgat agtttccatc tcttcctcct accttatttc ttcttcgctt ttttatcagc
    21301 agcgtggccg cgataagtac gagtcggtgc gaattcaccc agtttgtgac cgaccatttc
    21361 gtcggttaca aataccggaa cgtgctgacg accattatgg acagcgatgg tcaaaccgat
    21421 catgttagga aagatcgttg aacgacggga ccaagtgcgc aggggcttct tgtctccgct
    21481 ttccaccgct ttctctacct tcttcagcaa gtgcaggtca ataaaaggac ctttcttgag
    21541 agaacgtggc atggcttatc ctctaaaatt atttgctacg gcgacgtacg atgaatttat
    21601 cagtacgctt gttgctgcgg gtcttcttac ctttggtctg aacgccccac ggagttaccg
    21661 ggtgcttacc aaagttacga ccttcaccac caccatgtgg gtggtctacc gggttcatcg
    21721 cggtaccgcg aacggtcgga cgaacaccac gccagcgtgc agcacctgct ttacccagaa
    21781 cgcgcagcat atgctcagca ttgccaactt cgcccagagt tgcacggcag tctgcttcta
    21841 ctttacgcat ttcaccagaa cgcagacgca gggtgacata agcaccatca cgagcaacga
    21901 tctgaacgta agtaccagcg gaacgtgcca gctgaccgcc tttacctggt ttcatttcta
    21961 cgttatgaac agtagaacca accgggatgt tgcgcatcgg cagggtgtta cctggtttga
    22021 ttgcagcatc aacgccagac tgaatctggt cgccagcttt caggccttta ggggccagga
    22081 tgtaacggcg ttcaccgtct ttgtacagaa ccagcgcgat gttcgcggaa cggttcggat
    22141 cgtactcaag acgttcaaca actgccggga taccgtcttt gttgcgtttg aagtcaacaa
    22201 tacggtaagc ctgcttgtgg ccaccaccga tatgacgagt ggtgatacgg ccattgttgt
    22261 tacgaccacc ggatttgctg tttttttcca gcaacggagc aaaaggtttg cccttgtgca
    22321 gctcagggtt aaccacttta actacgtggc gacgacccgg agatgtcggt ttacatttaa
    22381 caactgccat tgtattactc ctccgactta ctcagcgccg ccaacgaagt ccagattctg
    22441 gccttctttc agggtgacgt aagctttttt ccagtcgcta cgacgaccga tacgctgtcc
    22501 gtgacgttta actttccctt taactaccag ggtgttaacg acttcgactt cgacttcaaa
    22561 cagtttctgc acagcagctt tgatttctgc tttggtcgcg tctttagcaa ctttgagtac
    22621 gatggtgttg gatttttcca tcgcagtaga cgctttttca gaaacgtgcg gtgcacgcag
    22681 caccttcagc agacgttctt cacgaatcat gccagcatct cctcaacttg cttaacagca
    22741 tcagcagtca ttacgacttt gtcgaaggcg atcaggctaa ccgggtcgat accagttgca
    22801 tcgcgtacgt caaccttgtg caggttgcgc gcagccagga acaggttctc gtccagctca
    22861 ccggtgatga tcagcacatc ttccagagcc atgtctttca gtttctgtgc cagcagctta
    22921 gttttcggcg cttctacaga gaacttctcg acaacgatca gacgatcctg acgtaccagt
    22981 tcggacagga tgcttttcag cgcgccgcgg tacatcttct tgttaacttt ttgactgtgg
    23041 tcctgcggac gagcagcaaa ggtcacgcca ccagaacgcc agatcgggct cttgatagaa
    23101 ccagaacgcg cacggccggt gcctttctgg cgccacggtt ttttaccgga accagttact
    23161 tcagcacgag tcttctgagc acgagtaccc tgacgagcac cagctgcata agcaacaaca
    23221 acctggtgaa ccagcgcttc gttgaaatca cgaccgaagg tagtttcgga aacagtcagc
    23281 gcgctctgcg cgtctttcaa tactaattcc attgctatct ccttacgcct tcacagctgg
    23341 tttaacgatc aggtcgctac cggttgcacc cgggacagcg cctttaacca gcagcaggtt
    23401 gcgctcagcg tcaacgcgta ctacgtcaag gctctgaacg gttacacgtt cgttacccat
    23461 ctgacctgcc attttcttgc ctttgaacac tttgcccgga gtctggttct gaccgataga
    23521 acccggaacg cggtgagaca aggagttacc gtgagtagcg tcctgggtac ggaagttcca
    23581 gcgcttaacg gtacctgcga aacctttacc tttagaggtg ccagttacgt caactttttt
    23641 aacgtcagca aacagttcaa cgctaatgct ctgacctaca gtgaactctt cgccttcagc
    23701 caggcggaat tcccacagac cacggccagc ttctacgcca gctttagcga agtgtccagc
    23761 ttcaggcttg gtcacacggt tagctttttt agcaccggtg gtcacctgaa tagcacggta
    23821 gccatcgtta gccaggtctt taacctgagt aacgcggttt gcttcaactt cgattacggt
    23881 tactgggata gaaacgccgt cttctgtgaa gatacgggtc atacccactt ttttaccgac
    23941 taaaccaatc attgtttcaa cctctcaatc gctcaatgac ctgattaacc caggctgatc
    24001 tgcacgtcta caccggcagc cagatccaga cgcatcagag catcaacggt tttctcggtt
    24061 ggctcaacga tgtcaaccag acgcaagtga gtacggattt cgtactgatc gcgcgcgtct
    24121 ttgttgacgt gcggggagat cagaacagtg aagcgctctt tgcgtgtcgg cagcgggatc
    24181 ggaccacgga cctgcgcacc agtgcgctta gcagtctcga cgatttccgc ggttgcttga
    24241 tcgatcagac gatgatcaaa cgctttcagg cggatacgga ttctttggtt ctgcatgaga
    24301 ccagagctcc aattatttta taaacgaaaa tgattactcc tcagacccat tacgattgat
    24361 gggagagtgt aaccgttctt acgtagctcc ccgattggga gcattgttag gtagccaaat
    24421 tcggctaact gaggttcaga ttgaacctgc tgtcaactac gacaagcccg cgcattatac
    24481 atacttaacc accgaacgca agcgtattgc tagtaaagta aacagccttt gggttatctc
    24541 tcaagttcgt tcagcgcatt tttgacacgc tctcttaaac cggctttctc gtcgctttct
    24601 tctacagtat tttcttcgtc ttcagaagaa gagggggcat tctcaaccgc gacgcctggt
    24661 tcatcttcct cctcgacgga ttcgacgacg gggagtggct gttttttgaa aaaatagtgt
    24721 tgtgttgatt gtttaaaagc gggcgtttca gcatctacag tgacttctgc ttttactatc
    24781 caaagtgccc atagctgccg ggcataccca ccaaccaaca ccattcctgc aaaccacagg
    24841 caaatcagca ataaacaaat gacatatatt atggtgctgt gtttttgtcg ggagaagata
    24901 ccagggtgac ccgtttcttt ctgctcacgc gcggcgatat agaattcaaa catgtcattt
    24961 ccccttcact gttgtttctg gggaaatatt tgcttcatcc cgatataagt aggcactctc
    25021 ccccgccacc tgccagagat gtactaacgt tgaaaagcca acaacaccgt cagttttcaa
    25081 gtgatggctt ttttgaaatt gctttatttt ttctaccaac aagggacgcc actctgccga
    25141 agattctgtc gaaatatgca atgcgcggtt taacatcgtt tccagccaga gaatctcttc
    25201 ctcgctgcta tcacgcgtaa tggtactctc cccttccggc gacattttcc acaacagaag
    25261 ataatcaccc gtccatacca actcaaacca tttatgtgtc agcgtccagg tttgctgccc
    25321 tacgagtaca tcaacggtag catcaccgac gcgaacgacc acaacaggca attttttgtc
    25381 cccgacttta agcgatgcga tccagggcaa attctgatct accaatgttt gtaaagaagc
    25441 gttcccactt ttacatacca gccccgctct tactgcctga tcgcaccagg ccgaatcggc
    25501 tggaacctcg tacccccata cgccgtataa cacgcttagc gcctcactat cgcgcatcac
    25561 cacatgagca atatccttag tcatatcttg tttaactact ggcgttacgg gtatcagcca
    25621 cgcaggaacg ggtaacggag ctgtgaaaga ggacagcagt agccaaccac aggtaagtgc
    25681 gagaatggtt ccactcatca ccacggcaag ctgcattttc cgggggcgct tatcacctaa
    25741 tacgcggtaa atcattcgcc attgttttgc agtgaaaacg ctggtctgct cggcttgctg
    25801 taataagcga attgtttcgc cggcaagcgc cagctttctg cgatctcccc gacataataa
    25861 agctatccgt ttcagaactc tggcggttaa aaggagatcg ggctggtcaa tattctgacc
    25921 ttcaaggatg cttgccttaa gctcgtgctc cgtcagacga cctgtcgaga caagttcaat
    25981 gccctgtttt ttaaaatcac gttcaacttt tcgatcgggt ctgccgatca gcaaaaattg
    26041 accgtcagga ataagagata tcgcgcgttg gagatcatca agtatgcggc ggtgattcag
    26101 gtgagcattt tcaataataa taagagggca gcgactatca gttcgatgaa gtgccagtaa
    26161 ttgacccgcc atctcatctc gcgtccaggc tctgtcacgg cagccctgtt cagccgcgtt
    26221 atcgtcggta acgatccatg cggccttacc ttgccaggaa acaacgaggc ttaaagtaat
    26281 cacacggcga tgtttatctt tttgtagttg ctgcgccagc caggtttttc cacttccagc
    26341 ctcacccaac aaatagcaca gacgccaggt ctgacgtttc tcacgcaacc aggaaagaat
    26401 gacttctctt ctcgtagaca tagaacttcc tgttttaatt attgattatc cttaatcata
    26461 aaaataatgt atgtatttat taattaaata catattacta atataaataa attttgcttg
    26521 attcatgcaa gcggcattaa tactatttat actaacgtca atatacaacc cacccaatct
    26581 attttattag aacatacatc gtgcccacac tacctttccc tttccatctg gcgaatcata
    26641 ataaagatgc tgccatcaat atattgatta tatttataag catagggttg attattttta
    26701 atgtgaatta tttccataca actatagtta aaaatgaaca gatcattaat caaccaacaa
    26761 atgcatttca gtctgaattc agccttgccg ctttatggcg caacgaaaat catgctgggg
    26821 taaaagatgc aaatcccgtg gcagttaatc aggaaacacc taagctctct atagcgctta
    26881 atggcattgt actcacatcg aatgatgaga cttcttttgt cttaattaat gaggggaacg
    26941 aacaaaagcg atactccctc aacgaagccc ttgagtcagc gccgggaacg tttattagaa
    27001 aaataaataa aaccagcgtt gtgtttgaaa ctcacgggca ttacgaaaaa gtaactctcc
    27061 atccagggct acctgacatc atcaagcagc ctgattcgga aaatcaaaat gtactcgccg
    27121 attatattat cgccacacct atacgtgatg gagaacagat atacggtctt cgattgaatc
    27181 ctcgtaaggg tctcaatgca tttaccacca gcttgctgca acctggggat atcgccctca
    27241 ggatcaataa tctttcgctg acccaccctg atgaggtttc acaggcatta agtttactgc
    27301 taactcaaca aagtgctcag tttacaattc gtcgcaacgg cgtaccccgc ttgataaatg
    27361 tttccgtcgc ggaacttaca ggaatgaatg gacagcgcaa tgaagggact caataaaatc
    27421 acctgctgct tgctggcagc actactcatg ccttgtgcag gacacgctga gaacgaacaa
    27481 tacggcgcta acttcaataa cgccgatatc cgccagttcg tggaaatagt gggtcagcat
    27541 cttggcaaaa cgatcctgat cgacccttcg gtacagggaa ccatttccgt acgcagtaat
    27601 gatacgttta gccaacagga gtactaccag ttctttttaa gtattcttga tctttacggt
    27661 tattccgtga tcacactgga caatggtttt ctgaaagtgg tgcgctcagc taatgtaaaa
    27721 acatcgccag ggatgattgc tgacagttct cgtccaggcg taggtgatga gttggtcacc
    27781 cgaatcgtac cgcttgagaa cgttcctgct cgtgacctgg ctcccctgct ccgccagatg
    27841 atggatgcgg gtagcgtcgg taatgttgtg cattatgaac cctccaacgt tcttattctg
    27901 accggtcgtg cctccaccat taataaactg attgaagtca taaagcgcgt tgatgtcatc
    27961 ggcacagaga agcagcaaat tattcatctg gaatatgcat cagcggaaga tctcgccgag
    28021 attcttaatc aattaatcag cgaaagccac ggtaaaagcc agatgcctgc cctcctttcc
    28081 gcgaagattg tggcggataa gcgaaccaac tctcttatca tcagcgggcc ggaaaaagca
    28141 cgccagcgca taacttcatt actgaaaagc cttgatgtag aagagagcga ggaaggaaat
    28201 acccgggtct attacctgaa atatgctaaa gccacgaatc tggtggaagt gctaaccggt
    28261 gtttccgaaa agctgaaaga tgaaaaaggg aattcgcgta agccttcttc tacttctgcg
    28321 atggataatg tggccattac cgccgatgaa cagaccaact cgttggtcat taccgctgac
    28381 cagtccgtcc aggagaaact cgccacggta attgcgcgtc tggatattcg ccgtgcacag
    28441 gtgctggttg aggcaatcat cgtcgaagtt caggatggaa atggactcaa cctcggcgtg
    28501 caatgggcga ataaaaacgt tggcgcacag caatttacca atacgggatt accggttttt
    28561 aacgctgcgc aaggtgtagc tgattataaa aagaatggcg ggatcaccag cgcgaatcct
    28621 gcctgggata tgtttagcgc ctacaatggc atggctgcag gcttcttcaa tggcgactgg
    28681 ggcgtattgt tgactgcgct ggccagtaac aataaaaatg acatcctcgc caccccgagc
    28741 atcgtaacgc tggataataa actcgcgtcc ttcaacgttg gtcaggatgt gccggtgcta
    28801 tccggctctc agaccacttc tggggataac gtctttaata ctgtcgaacg caaaacggtg
    28861 ggcacaaaac tcaaagtcac tccacaggtc aatgaaggcg acgcggtact gctcgaaata
    28921 gagcaggagg tttccagcgt tgactcttcg tctaattcga cgctcggccc gacatttaac
    28981 acccgtacta ttcaaaacgc cgtgctggtt aaaaccggtg aaacggtggt cctgggcgga
    29041 ttgctggatg atttttctaa agagcaagtg tcaaaggttc ctctgcttgg cgatattcct
    29101 ttagtgggac aactcttccg ctatacctcg accgagcgcg ctaaacgcaa cctgatggta
    29161 tttatccgcc cgacgattat ccgtgacgat gatgtttatc gctcactgtc aaaagagaaa
    29221 tacacccgct accgtcagga gcaacagctg cgaatcgacg ggaaatcaaa agcgctggtt
    29281 ggctcggaag atttgccggt gctgaatgag aacacgttca acagtcacgc ccctgcgcca
    29341 tcgtcacggt gaggcattct tatgagaatt cactcaccgt accccgccag ttgggcgctg
    29401 gcacaacgaa ttggttatct ctattcagag ggcgagatta tttatctcgc cgatacgcca
    29461 ttcgaacggt tactcgatat tcaacgtcaa gttggccagt gccagaccat gaccagcttg
    29521 tcacaggctg attttgaagc ccgactggaa gcggtgttcc atcagaatac cggtgagtcg
    29581 caacagattg cgcaggatat cgatcaatcc gtcgatcttc tctcgctttc ggaagagatg
    29641 cccgcaaatg aagatctcct gaatgaagat tcagcggcac cggttatccg cttgatcaat
    29701 gctattttga gtgaggccat caaagaaacc gcctccgata tccacattga aacctatgaa
    29761 aaaacaatgt cgatccgttt tcgcatcgac ggcgttttgc ggacaatttt acagcccaac
    29821 aaaaaactgg cggcactgct tatctcccga attaaggtca tggcgcgtct tgatatcgcc
    29881 gaaaaacgta ttccacagga tggaagaatc agtttgcgta tcgggcgacg taacatagat
    29941 gtccgcgtat ccacactgcc atccatctat ggtgaacgtg ccgtgctccg cctgctggat
    30001 aaaaacagcc tccagctttc attgaacaac ctggggatga cggcagcgga taaacaggat
    30061 ttagaaaatc tcattcagct tccgcacggt attatcctgg taacagggcc gacaggctcc
    30121 ggtaaaagca ccacgctcta cgccattctt tcggcgctta atacccctgg ccgtaatatt
    30181 ctgacggtag aagatcccgt ggaatatgaa ctggaaggca taggacaaac tcaggtgaat
    30241 acgcgcgtgg atatgtcttt cgctcgcggc ctgcgcgcca tacttcgcca ggacccggat
    30301 gtcgtcatgg tgggggagat tcgtgataca gaaaccgcgc aaattgcggt tcaggcctcg
    30361 ctcacaggcc atctggtact ctcaacactc cacactaaca gtgcatcagg cgcagtcacc
    30421 cggctacgcg acatgggcgt tgaatcattc ctgctttcgt cttcacaggc agggattatc
    30481 gcgcaacgtc tggttcgtcg tctgtgtccg caatgccgac aattcacgcc cgtatcgccc
    30541 caacaagcgc agatgtttaa atatcatcag ctcgcggtga caacaattgg cactcccgta
    30601 ggttgccctc attgccatca atccggttat caggggcgca tggcgatcca cgaaatgatg
    30661 gtggtgacgc cggaattgcg ggccgctatt catgaaaatg tggatgaaca agcactggag
    30721 cgactggtcc ggcaacaaca caatgcctta atcaaaaatg gcctgcaaaa agtgatacgc
    30781 ggtgacacct cctgggatga ggttatgcgc gtcgccagtg ccacgctgga gaacgaagca
    30841 tgaattatcg ctatcgcgcc atgaccctgg atggtcaaaa attgcagggg atcattgatg
    30901 ctaacgatga acgtcaggca cgactgcggc tgcgtgaaga agggcttttc ctgttggata
    30961 ttcgccccca aaaaagttcg ggagtaaaaa cacgtcgccc gaggatcagc catagtgaac
    31021 tgacgctttt cacccggcag ttggcaacct taagcgcagc ggcattaccc ctggaagaga
    31081 gccttgccgt aattggtcaa caaagcagta ataatcgact ggctgacgtg ttaaatcagg
    31141 tacgcagcgc catccttgaa gggcatcccc tttccgatgc gttacaacat tttcccacgc
    31201 ttttcgattc gctctaccgt accctggtaa aagcgggcga aaagagcggt ctgctggccc
    31261 cggtgttgga aaagctggct gattacaatg aaaaccggca gaaaatccgc agcaagctca
    31321 ttcagtcact gatctacccc tgcatgctca ctacggtggc gattgtggtc gtgattattc
    31381 tcctcactgc tgtcgtgccc aaaattaccg aacagtttgt gcatatgaag cagcaactgc
    31441 cactgagtac acgcattctt ttaggtctga gcgacacgtt gcaacatacc ggcccgacat
    31501 tactagcgac agtgtttatt gtcgctgtag gtttctggct ctggttaaaa cgcggcaata
    31561 accgccaccg ttttcatgcc atgttgctgc gcgttgcact catcggcccg ctgatttgcg
    31621 ccattaacag cgcacgctat ctccgcactt taagtatttt gcaatccagc ggcgtccctc
    31681 tgctggatgg gatgaatttg tccaccgaaa gtctcagcaa cctcgaaatc cgcaagcgtc
    31741 tggcaaatgc ggcagaaaac gttcggcagg gtaacagcat tcatctttcg ctggaacaaa
    31801 ccgcaatctt cccgccgatg atgctctaca tggtggcctc tggcgaaaaa agcgggcagc
    31861 tcggcacatt gatggtcagg gccgcagata accaggagac actccaacaa aatcggatcg
    31921 ccttaacgct ctccatcttc gagcccgcac tcattattac gatggcactg atcgtcctgt
    31981 ttattgtcgt ctcggtactc caacctcttc ttcaacttaa ctcaatgatt aactaaggaa
    32041 aaacaatgcg cgcaacggat aagcaacgcg gttttacatt actggaaatt atggtggtga
    32101 tcgtcattat cggcgtactt gccagcctgg tggttcccaa cctgatgggc aataaagaaa
    32161 aggcggataa acaaaaagcc gtcagcgata ttgtggcgct ggaaaacgcc ctggatatgt
    32221 acaagctcga caatcatcgc tatccaacca caaatcaggg gcttgaatcc ttagtcgaag
    32281 cgccgacact gccaccgctg gctgcaaact ataacaaaga aggttatatc aagcgtctgc
    32341 ccgccgatcc ctggggcaat gattatgtcc ttgttaatcc tggtgaacac ggcgcatacg
    32401 atctgctttc agcagggccg gacggtgaaa tgggaaccga ggacgacatc accaactggg
    32461 gtttgagcaa gaagaaaaaa taagtcagcg atgaatcagc aacgcgggtt taccctgctg
    32521 gagatgatgc tggtgctggc gctggtcgct atcacggcaa gtgtggtgct cttcacatat
    32581 ggtcgggagg atgccgccag cactcgtgcg cgcgaaacag cggcgcggtt taccgctgca
    32641 cttgaattag ccatcgaccg cgccacgctc agtggtcagc ctgtcggtat ccatttctct
    32701 gattcagcat ggcgcattat ggtaccgggc aaaacaccat cagcctggcg ctgggttcca
    32761 ttacaagaag atgccgcaga cgagagcaaa aatgactggg gtgaagaact ctcaattcaa
    32821 cttcagccat ttaaacctga tgacagcaac caaccgcagg tggtgattct ggccgacgga
    32881 caaatcactc ccttctcttt gttgatggct aatgccggga ccggtgagcc gctcctgacg
    32941 ttagtcagtt caggctcatg gcccctggat caaacgctcg ccagagatac ccgcccatga
    33001 acaaacaatc agggatgaca ctgcttgaag tcttactggc gatgagtatt ttcaccgctg
    33061 ttgcgctgac tttaatgagc agtatgcaag ggcaacgaac tgccatcgag cggatgcgta
    33121 atgagacgct ggcattgtgg atagcggata accagcttca gtcacaagat tcattcgacg
    33181 aggaaaacac gtccagttct ggcaaagagt taataaatgg cgaagagtgg aactggcgca
    33241 gcgatatcca ctcaagcaaa gacggcacgc ttctggaaca caccataacg gtgacattac
    33301 ccagcggtca gacaacctcg cttacgcgct atcaaagcat caataataaa tccgggcagg
    33361 cacaagatga ttaatcgcca gcagggtttc acattgctgg aagtcatggc ggcgctggcg
    33421 attttctcaa tgcttagcgt gctggcgttt atgatttttt ctcaagcttc cgagttgcat
    33481 caacgttcac agaaagaaat ccagcagttc aatcagctac agcgcaccat caccattctt
    33541 gataacgatc tcctgcaact ggtcgcccgg cggaatcgga gtacggacaa aatcatggtg
    33601 ttgggtgaag aagcgatttt taccacgcaa agtcgcgatc cgctggcccc tctcagtgaa
    33661 gcacaaacct tactcactgt tcactggtat ctgcgaaatc atacgctcta ccgtgctgtt
    33721 cgtacttctg tggatggcag gaaggatcaa cccgcccagg cgatgctgga acatgtcgag
    33781 agctttcttc tggaaagtaa cagcggagaa agccaggagc ttcctctctc agtcacgtta
    33841 catctgaaga ctcagcaata tggggcgtta cagcgccgtt ttgcattacc tgagcagtta
    33901 gcgagagaag agtcgccagc tcagacgcag gccgggaata ataaccatga ataatgaaca
    33961 acgcggcgta gcactgctca ttgtactcat gttactggcg ctcatggctg ctctggctgc
    34021 agacatgacg atcagttttc acggccagct tcatcgaacc cgccaggtaa accatcattt
    34081 gcaacggcaa tatgatattg agctggcgga gaaacttgcg cttgccagcc tcaccctgga
    34141 cgttaaagat aacgacagac aaaccacgtt gcagcagtac tgggcacaac cgcaacagtt
    34201 gcagctggaa aatggcaata cagtgaagtg gcagttacgc gatgcccagc actgttttaa
    34261 tctcaatgcc ttagcaaaaa tctctgacgc cccgctggct tcccctgatt tccccgccca
    34321 ggtattcagt gccctgctga ttaacgccgg tatcgatcgc ggaaacaccg atgagatcgt
    34381 gcaatcaatt gctgactata ttgatgccga tgattcgcca cgctttcacg gcgcagaaga
    34441 taacttttac caaagccaaa cgccgcctcg ccatagcgcc aatcagatgc tttttctgac
    34501 cggtgaattg cgtcagataa aaggcataac cgaaaacatc tatcagcggc ttatcccgta
    34561 tgtctgcgtt ctcccgacct cggaacttag catcaatctt aatatgctaa cggagaacga
    34621 tattccgctc ttcagagcgt tattcctcaa taacatcacg gatgctgacg ctcgggtctt
    34681 attacaaaaa aggcccgggg aaggctggct aaccacagat gcatttcttt actgggcgca
    34741 gcaggatttc tccggcgtga agcccctggt tgctcaggtg aaagggcatc tctttcccta
    34801 tagccgctat tttacgctga gtacggaaag tatcagcgac gaacaatccc agggatggca
    34861 aagccatatt tttttcaacc gtaaacagca gagcgcccaa atctatcggc gtacgctgca
    34921 actctactaa tcataaggat cgtgatgcct gaatcgttga tggtaatacg ttctttctcg
    34981 acgctgcgta agcattggga atggatgacg ttctccgcag atagcgtatc ttcggtacat
    35041 acgctcacag acgatcttcc gctggagtcg ctggccgatc agcccggtgc tggaaatgtt
    35101 caccttttga tcccacctga gggtctttta tatcgttccc tcacgctgcc caacgccaaa
    35161 tacaaactca ccgcgcaaac attacagtgg ttggcggagg agacattacc cgacgccagc
    35221 caaaattggc actggacggt tgtcgataaa caaaacgaaa gtgtcgaggt gataggcatt
    35281 aagtcggaga aactcagtcg ttatcttgag cgtttacata ctgccggact caacgtgacg
    35341 cgggttttgc cagatggctg ctatcttccc tgggaagtag atagctggac tctggttaac
    35401 cagcaaacaa gctggcttat tcgcagtgcc gcacatgcct tcaacgaact ggatgaacac
    35461 tggttacagc atcttgctaa ccagtttccg ccagagaata tgcgttgtta tggtgtagcg
    35521 cctcacggcg ttgcagcggc caatccactt atccagcatc ctgaaatacc atcactgagt
    35581 ttgtattctg ccgatattgc attccagcgc tatgacatgc tgcatggtgt ttttcgtaaa
    35641 cagaaaactg taagcaaatc gggaaaatgg ctcgcccgcc tggcagtaag ctgtctcgtt
    35701 ctcgccattc tttcttttgt tggcagccgg ggcatcgcgt tctggcaaac gcttaagatt
    35761 gaagatcaac ttcagcaaca acagcaagaa acctggcaac gttatttccc gcagatcaaa
    35821 cacacccaca actttcgttt ttactttaag cagcaactcg ctcagcaata tcctgaagct
    35881 gtaccgttgc tctatcattt acaaacactt ctgcttgaac accctgaact gcagttaatg
    35941 gaggcgaact atagccagag gcaaaagtcg ctgaccttaa aaatgagtgc taagagtgaa
    36001 gccaatatcg atcgtttctg tgagttaaca cagtcctggc ttccgatgga aaaaacagaa
    36061 aaagatccgg tcagtggtgt atggacagta aggaactcag ggaaatgatt aaatcatggt
    36121 gggcagaaaa atccacatcg gaaaaacaga tcgttgcggc attggcggtt ctctcgctcg
    36181 gcgttttttg ttggcttgcg gtgataaaac cgatagatac ctacattgaa gagcatcagt
    36241 cccacgcgca aaaaattaag aaggacatca aatggatgca ggatcaagcg agcacccatg
    36301 gtttactcgg tcatccagcg cttacgcagc ccatcaaaaa tatccttctc gaagaagcaa
    36361 aacgtgagaa tttgactatc acgctggaaa acggtccgga caatactctg acaattaacc
    36421 ctgtcacagc tcccctcgaa aacgtctccc gctggctcac tacggcacag ataacatacg
    36481 gtattgttat tgaagacctt cagtttactc ttgccggaaa cgaagagatc accctcaagc
    36541 atttgtcctt cagggagcaa caataatgac aatgctactg ccgctcttca ttctggttgg
    36601 ttttattgca ggttattttg ttaatgtcat ggcctatcac ctctcgcctt tggaggataa
    36661 aacggcgtta acgtttcgcc aggtattggt tcatttctgg caaaaaaaat atgcctggca
    36721 tgatacagtg cccctgatac tttgcgttgc tgctgctatc gcctgcgccc tggcaccttt
    36781 cacgcccatt gtgactggcg cactctttct ctatttctgt ttcgcactca cgctcagtgt
    36841 tattgatttt cgcactcagc tcctgcccga caaactcacc ttaccgctgc tctggcttgg
    36901 cttggtattt aatgcgcagt ctggattaat tgatttacat gatgcggttt atggcgcggt
    36961 cgctggctat ggggtgctgt ggtgtgttta ctggggcgtc tggttagt
//
LOCUS       DAZGJT010000040        36828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZGJT010000040 DAZGJT010000000
VERSION     DAZGJT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.040"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..287)
                     /gene="tssD"
                     /locus_tag="ACHKFK_002186"
     CDS             complement(<1..287)
                     /gene="tssD"
                     /locus_tag="ACHKFK_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO9052523.1"
                     /translation="MGDIVYLRIIGEKQGDISSGCGTYASVGNRWQVGHEDEIFAFAL
                     TNAITSTGKGVNLQGLQFCKLIDKSSPLLSNAINQNERLFIEIDLYRINKSG"
     gene            815..2185
                     /gene="aroP"
                     /locus_tag="ACHKFK_002187"
     CDS             815..2185
                     /gene="aroP"
                     /locus_tag="ACHKFK_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO9052524.1"
                     /translation="MEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSAG
                     PGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVLY
                     VLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIKV
                     IAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLEL
                     VGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIFH
                     ELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPVN
                     TILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQGV
                     VTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLVVLGIGYLFKEKTA
                     KAVKAH"
     gene            complement(2228..3082)
                     /gene="ampE"
                     /locus_tag="ACHKFK_002188"
     CDS             complement(2228..3082)
                     /gene="ampE"
                     /locus_tag="ACHKFK_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO9052525.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(3079..3630)
                     /gene="ampD"
                     /locus_tag="ACHKFK_002189"
     CDS             complement(3079..3630)
                     /gene="ampD"
                     /locus_tag="ACHKFK_002189"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO9052526.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            3718..4611
                     /gene="nadC"
                     /locus_tag="ACHKFK_002190"
     CDS             3718..4611
                     /gene="nadC"
                     /locus_tag="ACHKFK_002190"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO9052527.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4815..5255
                     /gene="ppdD"
                     /locus_tag="ACHKFK_002191"
     CDS             4815..5255
                     /gene="ppdD"
                     /locus_tag="ACHKFK_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO9052528.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLNTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            5265..6650
                     /gene="gspE"
                     /locus_tag="ACHKFK_002192"
     CDS             5265..6650
                     /gene="gspE"
                     /locus_tag="ACHKFK_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO9052529.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLACRGGEKVVLRLLQQVNQALNVNTLGMQPSQLVDFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHSRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            6640..7842
                     /gene="hofC"
                     /locus_tag="ACHKFK_002193"
     CDS             6640..7842
                     /gene="hofC"
                     /locus_tag="ACHKFK_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO9052530.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGVAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLASFSGEWGW
                     LLVLFGFLLAIANKLLMRHPTRLIARQKLLLRIPIMGSLMRGQKLTQIFTILTLTQSA
                     GISFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNVGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7877..8920)
                     /gene="guaC"
                     /locus_tag="ACHKFK_002194"
     CDS             complement(7877..8920)
                     /gene="guaC"
                     /locus_tag="ACHKFK_002194"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO9052531.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEKWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(9076..9120)
                     /locus_tag="ACHKFK_002195"
     CDS             complement(9076..9120)
                     /locus_tag="ACHKFK_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052532.1"
                     /translation="MIIKTLKMSARKRN"
     gene            9145..9765
                     /gene="coaE"
                     /locus_tag="ACHKFK_002196"
     CDS             9145..9765
                     /gene="coaE"
                     /locus_tag="ACHKFK_002196"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO9052533.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9765..10508
                     /gene="zapD"
                     /locus_tag="ACHKFK_002197"
     CDS             9765..10508
                     /gene="zapD"
                     /locus_tag="ACHKFK_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO9052534.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            10518..10715
                     /gene="yacG"
                     /locus_tag="ACHKFK_002198"
     CDS             10518..10715
                     /gene="yacG"
                     /locus_tag="ACHKFK_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO9052535.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10803..11201)
                     /gene="mutT"
                     /locus_tag="ACHKFK_002199"
     CDS             complement(10803..11201)
                     /gene="mutT"
                     /locus_tag="ACHKFK_002199"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO9052536.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKVEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRVYAG"
     gene            complement(11261..13966)
                     /gene="secA"
                     /locus_tag="ACHKFK_002200"
     CDS             complement(11261..13966)
                     /gene="secA"
                     /locus_tag="ACHKFK_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO9052537.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLAAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(14028..14540)
                     /gene="secM"
                     /locus_tag="ACHKFK_002201"
     CDS             complement(14028..14540)
                     /gene="secM"
                     /locus_tag="ACHKFK_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO9052538.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14771..15688)
                     /gene="lpxC"
                     /locus_tag="ACHKFK_002202"
     CDS             complement(14771..15688)
                     /gene="lpxC"
                     /locus_tag="ACHKFK_002202"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO9052539.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15789..16940)
                     /gene="ftsZ"
                     /locus_tag="ACHKFK_002203"
     CDS             complement(15789..16940)
                     /gene="ftsZ"
                     /locus_tag="ACHKFK_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO9052540.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQAQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(17001..18263)
                     /gene="ftsA"
                     /locus_tag="ACHKFK_002204"
     CDS             complement(17001..18263)
                     /gene="ftsA"
                     /locus_tag="ACHKFK_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO9052541.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(18260..19090)
                     /gene="ftsQ"
                     /locus_tag="ACHKFK_002205"
     CDS             complement(18260..19090)
                     /gene="ftsQ"
                     /locus_tag="ACHKFK_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO9052542.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPGESTQQQNQAQAEQQ
                     "
     gene            complement(19092..20012)
                     /gene="ddlB"
                     /locus_tag="ACHKFK_002206"
     CDS             complement(19092..20012)
                     /gene="ddlB"
                     /locus_tag="ACHKFK_002206"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9052543.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLNDKQLAEISSLGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPAGTFYDYEAKYLS
                     DETQYFCPAGLEALQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(20005..21480)
                     /gene="murC"
                     /locus_tag="ACHKFK_002207"
     CDS             complement(20005..21480)
                     /gene="murC"
                     /locus_tag="ACHKFK_002207"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO9052544.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21534..22601)
                     /gene="murG"
                     /locus_tag="ACHKFK_002208"
     CDS             complement(21534..22601)
                     /gene="murG"
                     /locus_tag="ACHKFK_002208"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO9052545.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQSQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22598..23842)
                     /gene="ftsW"
                     /locus_tag="ACHKFK_002209"
     CDS             complement(22598..23842)
                     /gene="ftsW"
                     /locus_tag="ACHKFK_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO9052546.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23842..25158)
                     /gene="murD"
                     /locus_tag="ACHKFK_002210"
     CDS             complement(23842..25158)
                     /gene="murD"
                     /locus_tag="ACHKFK_002210"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO9052547.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGGLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWVNDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLATRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(25161..26243)
                     /gene="mraY"
                     /locus_tag="ACHKFK_002211"
     CDS             complement(25161..26243)
                     /gene="mraY"
                     /locus_tag="ACHKFK_002211"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO9052548.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(26237..27595)
                     /gene="murF"
                     /locus_tag="ACHKFK_002212"
     CDS             complement(26237..27595)
                     /gene="murF"
                     /locus_tag="ACHKFK_002212"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO9052549.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKNTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIKLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDCVLSVGK
                     QSHAISTASGVGEHFSDKTALIARLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27592..29079)
                     /gene="murE"
                     /locus_tag="ACHKFK_002213"
     CDS             complement(27592..29079)
                     /gene="murE"
                     /locus_tag="ACHKFK_002213"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO9052550.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSAHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TDVNYHDSGATIRFSSSWGDGEIESRLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGYAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(29066..30832)
                     /gene="ftsI"
                     /locus_tag="ACHKFK_002214"
     CDS             complement(29066..30832)
                     /gene="ftsI"
                     /locus_tag="ACHKFK_002214"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO9052551.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMAEYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLTLSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30848..31213)
                     /gene="ftsL"
                     /locus_tag="ACHKFK_002215"
     CDS             complement(30848..31213)
                     /gene="ftsL"
                     /locus_tag="ACHKFK_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO9052552.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(31210..32151)
                     /gene="rsmH"
                     /locus_tag="ACHKFK_002216"
     CDS             complement(31210..32151)
                     /gene="rsmH"
                     /locus_tag="ACHKFK_002216"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO9052553.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLHVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(32153..32611)
                     /gene="mraZ"
                     /locus_tag="ACHKFK_002217"
     CDS             complement(32153..32611)
                     /gene="mraZ"
                     /locus_tag="ACHKFK_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO9052554.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(33213..34217)
                     /gene="cra"
                     /locus_tag="ACHKFK_002218"
     CDS             complement(33213..34217)
                     /gene="cra"
                     /locus_tag="ACHKFK_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO9052555.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(34299..34385)
                     /locus_tag="ACHKFK_002219"
     CDS             complement(34299..34385)
                     /locus_tag="ACHKFK_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO9052556.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(34397..34888)
                     /gene="ilvN"
                     /locus_tag="ACHKFK_002220"
     CDS             complement(34397..34888)
                     /gene="ilvN"
                     /locus_tag="ACHKFK_002220"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO9052557.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMC"
     gene            complement(34891..36615)
                     /gene="ilvI"
                     /locus_tag="ACHKFK_002221"
     CDS             complement(34891..36615)
                     /gene="ilvI"
                     /locus_tag="ACHKFK_002221"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO9052558.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTSGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHLQLKEAVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRTRQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPQELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36734..36766
                     /locus_tag="ACHKFK_002222"
                     /pseudo
     CDS             36734..36766
                     /locus_tag="ACHKFK_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         8867 a        10272 c         8962 g         8727 t
ORIGIN      
        1 ccgcttttat ttatacgata caaatcgatt tcaataaata accgctcatt ctgattgatg
       61 gcattagaca gtagcggtga gcttttatca atgagtttgc aaaattgtag cccctgtaga
      121 ttaacgcctt taccagtact ggtaatggcg ttggtgagtg caaaggcaaa aatttcatct
      181 tcatggccaa cctgccagcg attaccaacc gacgcatacg tcccacagcc tgaagaaata
      241 tcgccctgct tctcaccgat tattctcagg taaacaatat cgcccacttt atttcattcc
      301 cgtttatcct taactttttt gcattaaata gaaaacttat ttctcgatca ataagaaacg
      361 cttttaatga aaaattattt caattactta acgttttcac ttagctcgca atattatatt
      421 taaatactcg cgttacaacc ccattgtatt attaacatct atttattctt atatgacctt
      481 ttgtaagatt attgtgctgg tggctaaggc atggtgttca gcaggcagac aaatccgaca
      541 tatagctagt taaaacagta tgttatcaga cttaaagtat ctattaaatg tgatgaaaaa
      601 cttacaattg cttttttttt atccagaaaa tcaacacatc tacataaagc aggtgcattc
      661 tcgcgcatat accattatct tgtgatagtt gaaagtacta tcatcataat tcagttacat
      721 aaaatgtaaa gaaaccaata caacaaacgg aattacaaaa ttacacacgc attgctgcgt
      781 agttcaaaaa aacaacacaa cacgaggttt cataatggaa ggtcaacagc acggcgagca
      841 gctaaagcgc ggccttaaaa accgccatat tcagcttatc gcgctgggtg gcgcgatagg
      901 gaccgggcta ttcctgggta gcgcctccgt aatacagtcc gcagggccag ggattatcct
      961 gggttacgcc attgctggtt ttatcgcctt tctgatcatg cgtcagctag gtgaaatggt
     1021 agtcgaagaa cctgtcgcag gctcctttag ccactttgct tataaatact ggggcagttt
     1081 tgccggtttc gcctctggct ggaactactg ggtactgtac gttttagttg ccatggcaga
     1141 gctgactgcc gtgggtaaat acattcagtt ctggtatccg gaaatcccaa cctgggtttc
     1201 tgccgccgtg ttctttgtgg tgattaacgc catcaacctg accaacgtta aagtgtttgg
     1261 cgagatggag ttctggtttg ccattatcaa agttatcgcg gtggtagcga tgatcatctt
     1321 cggcggctgg ctgctattca gcggcaacgg cggcccgcag gcgagcgtta gcaacctgtg
     1381 ggatcagggt ggtttcctgc cgcacggctt caccgggctg gtgatgatga tggcgattat
     1441 catgttctct ttcggtggtc tggaactggt ggggatcacc gcagcagaag cagataaccc
     1501 ggagcaaagt attccaaaag cgactaacca ggttatctac cgcatcctga ttttctatat
     1561 tggttcgtta gccgttctgc tctcgctgat gccgtggacc cgcgttactg ctgataccag
     1621 tccgtttgta ctgatcttcc acgaattggg cgataccttt gtggctaatg cgctgaacat
     1681 cgtggtactg actgctgcgc tctccgtgta caacagctgc gtatattgca acagccgtat
     1741 gctgtttggt ctggcacaac agggcaacgc gccaaaagcg ctggcgtctg tcgataaacg
     1801 cggcgtacca gtcaacacca ttctggtgtc tgcgctggtt acggcattgt gcgtgctgat
     1861 taactaccta gccccagagt ccgctttcgg actgttaatg gcgctggtgg tttctgcact
     1921 ggttattaac tgggcgatga ttagcctggc acatatgaag ttccgtcgcg ccaagcagga
     1981 acaaggcgtg gtaactcgct tccctgctct gctttatccg ctgggtaact ggatctgcct
     2041 gctgtttatg gcggcggtac tggtgattat gctgatgacg ccggggatgg cgatttcggt
     2101 ttacctgatc ccggtatggc tggttgtgtt aggtatcggc tatctgttta aagagaaaac
     2161 ggcaaaagca gtaaaagcgc attaatctct ctacgccctc acccgtgcag ggtgagggca
     2221 ataatcttta caccaacgcc ccgtaaatcg tcagtagcgc aattaccacc acgaccacga
     2281 acgaggtttt cttcgccatg gaaaccgctg ccttcggcgt ctccacctta tcgacatgcg
     2341 gttcacgcgc cagagagaac tgtgccagac gcgttaatac ctgatattgc gaagtatgga
     2401 aatcacccag cgaagcaaac caggccggta acgctttctc cccatgaccg atcaaggcat
     2461 ataccacacc cgcaagacga accggcaccc aatccagtac atgaagcacg gcatcaatgc
     2521 cggactgtaa acgatgatgc ggcgtctggt aacgcgccag ccagtattgc catgcacgca
     2581 aaaacgcata ccccatcagc gtaacgggtc cccaggttcc ccccacaatc agccagaaca
     2641 gcggtgcaag ataaaaacga aagttaatcc acagcaatgc attttgcagc tcacgcaaat
     2701 actcacgttc atcgcagcct gccgggacgc cgtgaatcat ggtgagttcg ccagccatcg
     2761 tggcacgggc atggctatca ttacgtgaag cagctgtcag ataagcatga taatgaagac
     2821 gaactttacc tgcgccaata cacagcaaac caatcagcag ccacaccagc agcgtgggaa
     2881 cgttgaacaa tactccctgc aatgcgcgta acagtaaaaa agtcacgccc atcgcaataa
     2941 tggtcatgcc taatgtgcgc ccgagagaaa aatgtttcac ccgccgaaag aacgcttcaa
     3001 gacgatgatc aagctgccag tgctcgccca acttaaacag gcgctcgaaa attaacacca
     3061 gtaaggttgt aaatagcgtc atgttgtctc cttgctgacc agtgcacgaa accgtgccca
     3121 atcaaatgca gggccgggat cggttttccg atccggcgca atatcacaat ggcccgtcat
     3181 gttattagcg atatctggat agcgatcaat cagtgcccgc gtaaccgccg caagctgttg
     3241 atactgcgca tcggtatacg ccagcgtatc ggtgccttcc agctcaatcc caatagaaaa
     3301 atcattgcag cgttcacgcc cctgatactg agagactccc gcatgccagg cacgtttatc
     3361 gaaaggaaca tactggacta tttcaccatc acggcgaatc aaacagtgag cggagacgcg
     3421 caaatgggcg atctcagcaa agaaaggatg cgcctgcgga tcaatagttc cagtgaataa
     3481 tgcgtcgatc cacggaccgc caaactcgcc tggcggcagg ctaatattgt gcaccaccag
     3541 cagggtgggt gtttcgtcat ccgggcggca atcgtaatgt ggtgagggaa cgcggcgcgc
     3601 gccaaccagc cacccctgtt ctaacaacat gcaggatctc cttatatgtg gtgctaatac
     3661 ccggttcaga gtagcatgtt tctaccttat gattcgttag ctatctggag ttttaacatg
     3721 ccgcctcgcc gctataaccc tgacacccga cgtgacgagc tgctggaacg cattaatctc
     3781 gatatccccg gcgcggtggc ccaggcgcta cgggaagatt taggcggaac agtcgatgcc
     3841 aacaatgaca ttacggcaaa acttttaccg gaaaactctc gctctcatgc cacagtgatc
     3901 acccgcgaga atggcgtatt ttgcggcaaa cgctgggttg aagaagtgtt tattcaactg
     3961 gcaggcgacg atgtcaccat tatctggcat gtggatgacg gcgatgtcat caacgccaat
     4021 caacccttgt tcgaacttga aggcccatct cgcgtgctgt taacgggcga acgcactgcg
     4081 cttaattttg tgcaaactct ttcaggagtt gccagtaagg tacgccacta tgtcgaatta
     4141 ctggaaggca ccaacacgca gctgttggat acgcgtaaaa ccttgcccgg cctgcgttca
     4201 gctctgaaat acgcggtact ttgcggcggc ggagcgaatc accgcctggg gctttctgat
     4261 gccttcctga tcaaagaaaa ccatattatt gcctccggct cagtgcggca ggcggtcgaa
     4321 aaagcgtcct ggttgcaccc ggatgcgcca gtagaagtcg aagtagagaa tctggaagaa
     4381 cttgatgaag ctctgaaagc aggtgccgat atcatcatgc tggataactt cgaaacagaa
     4441 cagatgcgcg aagccgtcaa acgcaccaac ggcaaggcgc tactggaagt gtctggcaac
     4501 gtcactgaca aaacactgcg cgaatttgcc gaaacgggcg tggactttat ctccgttggt
     4561 gcgctaacta aacacgtaca ggcactcgac ctttcaatgc gttttcgcta attgctaaca
     4621 gatttatccg ctccatcgcg ggcggataaa tctccccttc cccaactttc ttcgtaacgc
     4681 ctcgcaaatt tcttgtcttt cgctgcaacg aaataaaaaa cctcggaagc tgctttcccg
     4741 tttcgttttt tgcccctcgc ttgtgcttat ggcgtttgcc aaagtagcgc caacccaatc
     4801 aaggagcgaa acagatggac aagcaacgcg gttttacact tatcgaactg atggtggtta
     4861 ttggcatcat tgccatttta agcgccattg gtattcccgc ttatcaaaac tacctgcgca
     4921 aagccgcact caccgacatg ctacaaacct ttgtgcctta ccgtaccgcc gtagagttgt
     4981 gcgcgctgga acatggtgga ttaaatacct gcgacggtgg cagcaatggc attccctcgc
     5041 ctaccaccac ccgctatgtt tcagccatga gtgtggcaaa gggcgtggtg tcgctgaccg
     5101 ggcaagaaag tctcaatgga ctaagcgtcg tcatgacgcc gggttgggat aacgcaaacg
     5161 gcgtcaccgg ctggacgcgt aactgcaata ttcaaagtga cagcgcattg cagcaagcct
     5221 gcgaagatgt cttccgcttt gatgacgcca actaaggagc ggcaatgaat attccacagc
     5281 tcactgccct gtgtctgcgt tatcagggag tcttgctgga tgccagcgaa gaggtggttc
     5341 atgttgcggt agtcgatgca ccttcgcatg agctactgga cgcattgcat ttcgctacca
     5401 ccaaacgtat tgagatcacc tgctggacac gccaacaaat ggaaggtcac gccagtcgca
     5461 cacaacagac attgcccgta gctgttcagg agaagcatca gcccaaagca gagttgctga
     5521 ctcgaacgtt acaatctgcg ctagaacaac gcgcgtctga tattcatatc gaaccagcgg
     5581 acaatgccta ccgcatccgc ttgcgtatcg acggcgtatt gcatccttta ccggacgttt
     5641 caccggatgc cggagtcgca ttaaccgcca gattaaaagt gctgggaaac ctggatattg
     5701 cggaacatcg cctgccgcag gacgggcaat tcactgtcga actggcagga aacgccgtct
     5761 catttcgtat tgcgacctta gcatgtcggg gtggtgaaaa ggtggtatta aggttgttac
     5821 agcaggtgaa ccaggcactg aatgttaaca cgcttggaat gcagccgtca caactggtgg
     5881 actttgctca tgccttgcaa caaccacagg gactggtgct ggtaactggc cctacaggca
     5941 gcggcaaaac ggtcacgctt tatagtgccc tgcaaacgct gaataccgct gacattaata
     6001 tttgtagcgt cgaagatccg gttgagatcc ccatagccgg attaaaccag acgcaaatcc
     6061 attcgcgtgc cggactcacc tttcagggcg ttttgcgtgc gttattgcgc caggatcctg
     6121 acgtcatcat gatcggagag atccgcgatg gcgaaacggc agaaattgcc ataaaagccg
     6181 cgcaaaccgg tcacctggtg ttgtctaccc tacacactaa ctccacctgc gaaacgctgg
     6241 tacgtttaca gcaaatggga gtcgcccgct ggatgctatc atcggcgctt acgctggtaa
     6301 tagcccagcg tctggtacgc aaactttgcc cgcattgtcg ccggcagcaa ggggagccca
     6361 tccatattcc agacaatgta tggccatcgc cgctgcccca ctggcaggca cccggttgtg
     6421 tacattgcta ccacggtttt tatggtcgca cggccttatt tgaagttctg cccataacac
     6481 cggtcatacg tcagcttatt tccgctaata ccgacgttga atcgctggaa acgcacgccc
     6541 gacaggcggg tatgcgaacg ctttttgaaa acggctgcct ggccgtggag caaggcttaa
     6601 ccacctttga agagttaatc cgcgtattgg ggatgccgca tggcgagtaa gcaactctgg
     6661 cgctggcatg gcataaccgg cgacggcaat gcgcaagatg ggatgctatg ggcagagagc
     6721 cgtgctttgc tgctcatggc actacagcaa cagatggtta ccccactaag cctgaagcga
     6781 atcgccatca attctgcgca gtggcgagga gataaaagcg cggaagtcat tcatcaactg
     6841 gcgacgctac tcaaagccgg gttaacgctt tctgaagggc tggctctgct ggcggaacag
     6901 catcccagta agcagtggca agcgttgctg caatcactgg cgcacgatct tgaacagggc
     6961 gttgcctttt ccaatgcctt attaccctgg tcagaggtat ttccgccgct ctatcaggcg
     7021 atgatccgca cgggtgaact gaccggtaag ctggatgaat gctgctttga actggcgcgt
     7081 cagcaaaaag cccagcgtca gttgaccgac aaagtgaaat cagcgttacg ttatcccatt
     7141 atcattttag cgatggcaat catggtggtt gtggcaatgc tgcattttgt actgccggag
     7201 tttgccgcta tctataagac ctttaacact ccgctaccgg cgctaacgca ggggatcatg
     7261 acgctggcaa gttttagtgg cgaatggggt tggttgctgg tgctattcgg ctttctgctg
     7321 gcgatagcca ataagctgct gatgcgccac ccaaccaggc ttatcgcacg gcagaaattg
     7381 ctgttacgca tcccgattat gggttcactg atgcggggac aaaaactcac gcagatcttt
     7441 acgattctga cgctgacaca aagtgcaggc atttcttttt tacagggcgt tgagagcgtc
     7501 agagaaacaa tgcgctgccc gtactgggtg caacttctga cacaaatcca gcacgatatc
     7561 agtaacggtc accccatctg gctggcgcta aaaaatgtcg gtgagtttag cccgctctgt
     7621 ttgcaattag tgagaacagg agaggcatcc ggctcgctgg atctcatgtt agacaacctc
     7681 gcccatcatc atcgggataa cacaatggcg ctggcggata acctcgcagc cttactggaa
     7741 ccggcgttgc tgatcataac gggaggaatt atcggtacgc tggtggtggc aatgtatctg
     7801 ccaattttcc atttaggcga tgcgatgagt ggaatgggat aacgctggcg tgttgctcca
     7861 cgccagcgtt ggtatattac aggttgttga agatgcggtt ttcttgttcc tgcacacgga
     7921 taaacgtggt gcgcttggtc agttctttca ggcgtgaagc cccaacgtat gtacaagctg
     7981 aacgcaggcc gcccaaaata tcacgcgcgg tattttcaac cgggcctcgc agcggcagct
     8041 taacggtttt accttctgct gcgcgatatt ccgcaacgcc gccaacgtga cgtttcatcg
     8101 cagactctga gctcatgccg tagaacagca taaatttctc gccgttctcc tcaacgatgc
     8161 gaccgccgct ctcttcgtgg cccgccagca tgccgccaag cattacgaaa tcggcaccgc
     8221 cgccaaaggc tttcgcgaca tcgcccggcg tggtgcagcc gccatcgctg acgatcattc
     8281 cgcccagacc gtgcgcagca tcggcgcatt cgatcaccgc agaaagttgc ggataaccga
     8341 cgcctgtttt gacgcgagtt gtacaaacag aacctgggcc aatgccaact ttaacgatat
     8401 cggcaccgga gaggataagc tcctcacaca tttcaccagt cactacgttg cctgcacaaa
     8461 tggttttggt cggccacgct tcacgcgctt tcgcaacgaa ctgcacgaag tgttcggaat
     8521 aaccattcgc cacgtcaata caaacgaaat ttaatgccgg gttcaggtcg agaatctgtt
     8581 tagttttttc gaaatccgca tcagacgtgc cggtagaaac catcacatgt ttcagcacat
     8641 cagcggaaga attgttgata aacgcttgcc acttttcgac agaatagtgt ttatgcacag
     8701 cagtcagaat atcaaaagaa gccagcgcag aggccataga aaatgtgcct acggtgtcca
     8761 tatttgcggc aataatcggc acgccggacc aactctgacc tgaatgtttg aaggtgaatt
     8821 gacgttccag ttcaacatcg gaacggcttt taagagtgga gcgtttaggg cggatgagaa
     8881 cgtctttaaa acctaacttc agatcttctt caatacgcat gtgcggattc ctggggttaa
     8941 tggctataag ggtaagagcg caactccagt gacgttatca tacgcactaa tcaatgcggc
     9001 gcaagactgc gaaattgtct ttttttacgc tacaatccca taaatttacc tggtaaatag
     9061 tgagcgaacg tttcatcaat ttcgctttcg tgcgctcatt tttaacgttt tgataatcat
     9121 gatgaattcc cgggaatata aattatgagg tatatagttg ccttaacggg aggcattggc
     9181 agtggcaaga gtaccgttgc caatgcgttt gctgatctcg gaattaacgt cattgatgcc
     9241 gatattattg cgcgtcaggt ggttgaacca ggtgcacctg cgctacatgc cattgctgat
     9301 cactttggcg ctaacatgat tgctgctgat ggaacattgc agcgccgggc cttgcgcgag
     9361 cggatcttcg ccaacccgga agagaaaaac tggcttaacg ccctgctgca tccgctgatt
     9421 cagcaagaga cgcaacacca gatccagcaa gctacttccc cctatgtact atgggttgtg
     9481 ccattgctgg tagaaaactc actgtataaa aaagcgaatc gagtgctggt ggtggatgtc
     9541 agcccagaaa cgcaacttaa gcgcaccatg cagcgcgacg atgtaactcg cgagcatgtc
     9601 gaacaaatcc ttgctgctca ggcaacgcgc gaagctcgcc ttgccgtggc agatgacgtc
     9661 attgataata acggcgcacc ggatgctatc gcatcggatg ttgcccgcct gcacgcacac
     9721 tatttgcagc ttgcgtcgca gtttgtctca caggaaaaac cgtaatgcag acccaggtcc
     9781 tttttgaaca tccactaaat gaaaaaatgc gtacatggct gcgcattgag tttttgattc
     9841 agcaactcac cgttaattta cccatcgttg accacgctgg cgcgctgcat ttcttccgta
     9901 atgtcagcga attactggat gttttcgagc gcggcgaagt ccgcactgag ctgttgaaag
     9961 aacttgaccg gcagcaacgt aaactccaga cctggattgg cgtgcccggc gtggaccaga
    10021 gccgtattga agcattaatt cagcagttaa aagcggcggg gagcgtatta atctccgcgc
    10081 cgcgtatcgg gcaatttcta cgtgaagatc gtttgattgc tctggtgcgt cagcgactga
    10141 gcatcccggg cggctgttgc agctttgatt tacctacatt gcacatttgg ctgcatctac
    10201 cccaggcgca gcgcgacagc caggtagaaa cctggattgc cagcctgaac ccgcttaccc
    10261 aggcacttac catggtgctg gatttaattc gccagtcggc ccccttccgt aaacaaacca
    10321 gcctgaatgg tttttatcag gataacggtg gcgatgccga cttgctgcgc ctgaatctgt
    10381 cgctcgattc acagctttat ccgcaaattt ccggtcataa gagccgtttt gccattcgtt
    10441 ttatgccgct ggacagtgaa aacggacagg taccagaacg tctggatttc gaactggcct
    10501 gttgctaagg agtaaaaatg tcagaaacta ttacggtgaa ttgcccaacc tgcgggaaaa
    10561 cggtggtgtg gggtgaaatc agcccgtttc ggccattttg ctccaaacgt tgtcagctga
    10621 tcgacctcgg agaatgggct gctgaagaaa aacgaatccc cagcagtggc gatctttccg
    10681 aaagcgatga ctggagcgaa gagccaaagc agtgacattc tatctgatgc gacgctggcc
    10741 tgataaaacg cgccagcgtc gcatcaggca tcatatgcaa atgtcggatg cagcgtgacg
    10801 ccttatccgg catacacacg cttaagtttc gcaattaccg gttcattggc tggcggaaaa
    10861 tcatcggcat taagaccgac cagcgacatc cactcaccgg gttgcccttc tttaccccac
    10921 ggctcccctt cccagctttc gaccagccag aaccacagag ttatatgcct gtccgggaat
    10981 tcatattcca gtttttcaaa tagcgaaaaa tgttgtgggg taatcccgac ttcttcctga
    11041 agttcacgca ccaccgcctg ttccggcgtt tcgcccattt caactttacc gccgggaaac
    11101 tccagtttat tcgccatgtg cgcatctgct gcgcgacgcg ttataaagat ttcattgttc
    11161 tcgttgcgaa taatacctac cgcaatttgc agctttttca ttgtcttaaa cctataaaaa
    11221 aggcgcagaa tcctgcgcct tttacttcaa ctgttagctt ttattgcagg cggccatggc
    11281 actgcttgta ttttttacca gaaccacacg ggcaaggatc gttacgtcct actttgcgtt
    11341 caccggtttg cgccgccagt gcagctgcgg ctgcagagtc gtcatcctga tggctaagct
    11401 gctgcatttg cgctaaacgc tcggcttcca tacgacgctg ttgttccagc tcctcaacct
    11461 cttcaggcat acgtacctga actttgctca gcgtactgat aacttcatat ttcaacgact
    11521 ccagcatcgc tgcaaacatg gagaacgatt cacgtttgta ttcctgcttc ggatctttct
    11581 gtgcatagcc acgcaggtgg ataccctgac gcagatagtc catcgctgcc aggtgctctt
    11641 tccacagaga gtcgagagtt tgcagcatga cgcctttctc gaagtgacgc atcatctcag
    11701 caccaaccac ttcttcttta cgctgataca cttcgatgga ctgcgccaga atgcgctcac
    11761 gcagcgtctc ttcatgcagt tctggttctt tatccagcca ctcggcaatt ggtaaatcga
    11821 ggtcgaaatc gttcttcaga cgttcctgca gccccggaat atcccacatt tcttccagcg
    11881 actgtggcgg aatgtaggca tcaatggtcg ctttgaacac atcttcacga atgctgttga
    11941 tggtttcgct cacatcgctg acatccagca gttcgttacg ctgggagtaa atggcgcgac
    12001 gctgatcgtt agccacgtca tcatattcca gcagttgctt acgaatgtcg aagttacggc
    12061 tttcaacttt acgctgggcg ttggcaatcg ctttggtcac ccacgggtgc tcaatggctt
    12121 cgcctggctt catacccagt ttacgcatca tgccggatac tcggtcggaa gcaaaaatac
    12181 gcatcagcgc atcttccatc gacaggtaga aacgggaaga accagcatcc ccctgacgac
    12241 cagaacgacc gcgcagctgg ttatcgatac gacgggattc gtgacgttca gtaccgatga
    12301 tatgcaggcc acctgctgcc agtaccgcat cgtgacgtac ctgccagtcg gctttaattt
    12361 tttcaatttg ctctgcagtc ggattttcca gcgcggcaac ttctgcctgc cagctaccac
    12421 cgagcacaat atcggtacca cgacccgcca tgttggtcgc gatagtcacc gcagccggat
    12481 aacctgcctg agcaacaatc gccgcttcgt tggcatggaa tttggcgttc aggacgttgt
    12541 gcttaatacc ggctttggtc agttcatttg acaccagctc cgatttttcg atggagattg
    12601 tacccaccag caccggctgg cctttcgcag tacgttcttt gatatcttca atgatcgcct
    12661 gaattttttc cgcttcagtc atgtagacca ggtccggcag atctttacga atcattggac
    12721 ggttggtcgg aacaacgacg gtatccagct tgtagatgga gctaaattcg aaagcttcgg
    12781 tatcagcagt accggtcatc cccgccagtt tttcatacag acggaagtag ttctggaagg
    12841 tgatcgacgc cagcgtctgg ttttcgttct ggatctgtac accttctttc gcttccacag
    12901 cctgatgcag gccatcggac caacgacgtc cctgcatggt acgaccggtg tgttcgtcaa
    12961 cgatgataac ttcaccatct ttaacgatgt agtcgacgtc gcgggtaaac agcgcatgag
    13021 cgcgcagcgc cgccgttacg tggtgcatca gcatgatgtt ggccggagag tacagagact
    13081 caccttcatc catgatgcct tctttaacca gcagttcttc aatcagaacc agaccacgtt
    13141 cggtcaggtt cacctggcga gatttttcat ccaccgagaa gtggccttcg ccctggaagg
    13201 tttcggagtc ttctttttcc tgacggatca ggtgtggaat aattttattc acgcgtttat
    13261 acatttccga gctgtcttct gccgggccgg aaatgatcag cggtgtacgc gcttcatcga
    13321 tcaggatgga gtccacttcg tccaccagcg catagtgcag tttacgctgt acacgttctt
    13381 caggactgaa cgccatgttg tcgcgcaggt agtcaaagcc gtattcgttg ttcgtaccgt
    13441 aagtgatgtc agctgcgtaa gcttcacgct ttgccggtgc tggcatgccc ggcaggttga
    13501 taccaactgt caggccaaga aattcaaaca gcggacggtt gttttcggcg tcacgttgcg
    13561 ccaggtagtc gttgacggta actacgtgca cgcctttacc ggttagtgcg ttcaggtaag
    13621 caggcagcgt tgcggtcagg gttttacctt caccggtacg catttcggcg atgcagcgtt
    13681 cgttaagaac cataccgccg agtaactgaa cgtcgaagtg acgcatacca aagacgcgct
    13741 tacttgcctc acgcaccacg gcgaaagctt ccgggatcag attttccagc acttcgcctt
    13801 tttccagacg tgcacgaaac tctgcggttt tccctttcag ttcttcgtcg gagagttttt
    13861 ccatctccgg ttccatggca ttgatgatgt tgaccacttt gcgcatccgg cgcagggtgc
    13921 gatcgttacg actaccgaaa actttagtta ataatttgat tagcataata aaatctcaaa
    13981 cgccccgcgt tgcggagtca ataaaatgaa gtaacggttt attgttgtta gctgaggcgt
    14041 tgagggccag cacggatgcc ttgcgcctgg cttatccaga cgggcgtgct gaattttgct
    14101 tgcggggtaa aatgcgcata atcaatgcga taaccctttt cagacggcgt gccttcctgg
    14161 gtcaacagcg cgctgagcgt atccagtaat gcaagatgtt gcgcctgaag aggcaaagat
    14221 tcttcagcaa cgggcagcgt ttgcggtgcc attgcgaagg aaagatgacg gattaccgtg
    14281 cgaatggcat gttgatgcca gtaatcaacg gaatagttcg aattcgggcg gcgcgtgttg
    14341 gcttccagca aggccaattg accaaagtta actttggctg aaggctcgtg gttgcgggtt
    14401 gtcgcttttg cgggcgcgtt tggttcggcg gcgttgctga gtgcaggcaa acctaaactc
    14461 gccgcgacca tccctaataa gagatgcggc cagaagtagc gtttaccaaa ctgtcgccag
    14521 cgcgtcagta ttccactcac gttattgcca tcccgatcgt attcaaacgt atttagcgca
    14581 caaatcttat cattaaagcc tgaagtccac agcacgaatg aagataagtt gtgcgtaaaa
    14641 gtgcttaaga aaacagcgtt cgcaccgttt tttatagctc ttataaaatt caaaaaaaaa
    14701 cgactggttc acctggccgg agagagtgcc agatttgcca gtcgaatttt atacgacagt
    14761 ataaatgtcg ttatgccagt acggctgaag gcgctttgaa ggccaacggc agttctgcgt
    14821 cgtcctggaa ggtcacatat tcccaggctt cctgtttcgc caggacagcc tgcagcagtt
    14881 tgttattcag tgcatgaccg gatttataag cggtaaatgc accgataata ttgtgaccac
    14941 acatgaacaa gtcaccgatc gcatcgagca ttttgtgacg cacaaattcg tcttcaaaac
    15001 gcaggccgtc ttcgttcagt acgcgataat cgtcaacaac gatggcacaa tcgaagctgc
    15061 cgcccaggca caaaccacgg gactgcagat attcgatatc acgcatgaaa ccgaacgtac
    15121 gcgcacggct gatctggcgc ataaatgcat cagcggagaa gttcatcgca tagcgctggt
    15181 tgctggaatc aatagccgga tggttaaaat cgatggtgaa atccagagaa aaaccattgt
    15241 acggcttaaa ttcagcccac ttatcgccat cttcgacacg aacagtttct ttaatgcgaa
    15301 caaatttctt ggcgcagttc aactcgtcga taccggcgtc aagcagcagg tatacaaacg
    15361 gagcggcgct gccgtccatg atcgggattt ccggcgcgtt aacttcgata acaatgttat
    15421 cgatgcccag gcccgcgaga gcagcattga ggtgctctac ggttgaaatc cgcacatcat
    15481 gctcgttgac cagacacgta cagagcatgg tatcacgcac agatttggca tcggccggga
    15541 aatctaccgg tggattcaag tcggtgcgac gatagatgac cccggtgttg gccggcgcag
    15601 ggcgtaacgt cagggtgact ttcttgccgg tatgtaaacc gacacccgtc gcctgaacga
    15661 tacgtttaag tgtcctttgt ttgatcatcg tattatctcg ccaaattacc tatccaaccg
    15721 aagtgtacta tacattcggc gggccagttt agcacaaaga gcctcgaaac ccaaattcca
    15781 gtcaattctt aatcagcttg cttacgcagg aatgctggga tatccagata atccggctct
    15841 ttcgcagttt gcggtgcatt gtcattcacg actttagcaa ccggcttctg ctcctgggtc
    15901 agcggagcca tcccgtgctg ctggtagcga tccatcactg gctgctgagc ctgcttattg
    15961 gtcaccagag tgatttcagg acgtttgtcc atgccgatac ctgtcgcaac aacagttacg
    16021 cgcagctcgt cattcatatc cgggtcaaga gaagtaccga taaccacggt cgcgttgtcg
    16081 gaagcaaatg cacggatggt gttacctacc gtttcgaact catccagacg caggtcgaag
    16141 cccgctgtga tgtttaccag tacgccgcgt gcgccagaca ggtcgatatc ttccagcagc
    16201 ggagaagaga tagccatttc agcagcttct tccgcacggt cttcaccgct cgccacgcca
    16261 gaacccatca ttgcgtagcc catttcagac attacggtgc gtacgtctgc aaagtccacg
    16321 ttcatcaaac ccggacgagt aatcagttcg gcgatacctt gcacagcgcc tttcagtaca
    16381 tcgttcgctg cgccaaacgc atccagcagg gagataccac ggcccagaac tttcagcagt
    16441 ttgtcgttcg ggatagtgat cagagagtcc acatgcttgg acagttcagt gatcccctgc
    16501 tccgcgaatg ccatacgctt cttgccttca aagttgaaag gcttagtgac gacagcaacg
    16561 gtcaggatac ccaaatcttt tgccacttca gcgacgactg gtgctgcacc tgtaccggta
    16621 ccaccaccca tacccgcagc aataaagacc atgtctgcac cttccagcgc cgcacgcagt
    16681 gcatcgcgat cctcatcagc cgcattgcgg ccaacttctg gattagcgcc agctcccagt
    16741 cctttggtga taccgctacc gatttgaatc gtctgtccaa ccgctgtttt acgcagcgct
    16801 tgtgcatcgg tatttaccgc gaagaattca acaccttcaa tgcgctcacg caccatgtgt
    16861 tcaacagcat taccgccgcc gccgccgacg ccgatgactt taatcaccgc gtcattggta
    16921 agttccattg gttcaaacat agtttctctc cgatttgtgc ctgtcgcctg aggccgtaat
    16981 catcgtcggc ctcataaaaa ttaaaactct tttcgcagcc aactattgag tcgcttgatc
    17041 cacgagccaa ctgatgctgt aacacgtttt tctacttcag cttcaccgtt aagatgtgac
    17101 tctttcccat agtgaagcaa tcccaccgcc gtcgaataat acggctcctg agcataatcc
    17161 gttaaaccgg taatgttcag cggcgcgccg atacgcactt gcgtatgaaa cacgcgctga
    17221 gcacaggctg caagaccttc gatctgcgct gcgccaccgg ttaatacaat gcctgccgcc
    17281 aggtgatgtt taaccccttg ttggcgaagc ttttcctgca actgcaatat ctcttcgttg
    17341 accagattga gcagctcggt atagcgcggc tcgatcacct ctgccagggt ctgacgttgt
    17401 agactccgcg gcggacgacc gcctacgctc ggcacttcca cgctctcatc ttttccaacg
    17461 atggagccca gcgcacaacc gtggcgaact ttaatcgctt cggcgtcgct tggcggtgtg
    17521 ccaaaggcgt aagcgatatc actggtcacg acattgccag cataaggaat taccttcgtg
    17581 tggcgcaatg ccccaccggt ataaacggcg atatccattg taccaccacc gatatcgacg
    17641 acgcagacac ccagttcacg ttcatcttcc gtcaataccg aataacttga tgccagtccg
    17701 gcaaatatca gttggtcaac tttcagccca caacgttcaa ccgctttgac gatgtttttc
    17761 gccatatcgt tgtgacatgt gatcaggtgc acttttgcct gcatccgcac gcccgaaagc
    17821 cctaccggat tcttgatccc ttcctgatag tcaatcgcat actcttgcgg gatcacatgc
    17881 agcacacgat gctcatcgcg cacacgcacc gatttcgcgg tatggacgac gttttccaca
    17941 tcttcttgcg tcacttcttc ttcagaaata ggcaccatac caatttcatt ctggcagctg
    18001 atgtgcttac cagaaagcgc cagatatacc gaagagatct gacaatctgc catcaattct
    18061 gcctggtcaa tggcgcgttg tacgcacttg accacggatt cgaggtcgtt caccccgcct
    18121 ttatccatac cacgcgacgg gcagctgccc acgccaatga tattgaccat accgtcgggc
    18181 agaacttccc ctactaaagc ggcaaccttc gcggtaccaa tctccagtcc tactaccagt
    18241 tttctgtccg tcgccttgat cattgttgtt ctgcctgtgc ctgattttgt tgctgagtag
    18301 attcccctgg cggcaagggc gcccagccta ctgccgctcc agagtcataa cgcaaatcaa
    18361 cgtagctaat ccgtttgcca tcggtttgcg cctgctgctg taaaaccgga taaagttcta
    18421 caaagcgagc caaacgtttc atcgtatcgc cccggccaag attgagctta atatcgttat
    18481 tcagcgtcaa ctgccaggaa cgtcgcgcgg tcatcgccgc ttccttcaga gtaaatctgt
    18541 cctttgccag catctgcccc atttcgcgat agccctgcaa cacttcattg gcgctgcctt
    18601 ccgggccata cagcattgga agcacctgct tgctggtgcg atctggcggc acgctgaagg
    18661 tgtttccttc cgcgtctacc atatgttgat cattccaccg cgcaatcggc acatattcaa
    18721 ccagatgaat cttcaattca tcaggccact gctttctgac gctcacctgc ttaatccacg
    18781 gcaggcgttg ttctatttgc gtctggatga tgttgacatc ctgggtcata aaggtacctg
    18841 gctcacccaa tgccaggatc gactgccgga tatcgtcatt acgcgtgtaa tggcgttcac
    18901 cggtcaacac cagctttgag agcggcaggc gttgcgcatc ttccatccag cccaacacga
    18961 cccagccgct caccaacact gtcgttaaaa cggtcagcag gaaaaggatc cccgccagac
    19021 gcgttccatt attgcggcga gaagaaacct cttcttcgct atttcgcgtg ttcagagcag
    19081 cctgcgacat attagtccgc cagttccaga attcgtacta ccaactgcga gaagctcata
    19141 cctgcctgac gtgccgccat cggtaccaga ctgtggctgg tcatacccgg tgaggtattg
    19201 gcttccagca gataaaactg tccatcgctg tccagcataa cgtcaatacg tccccatcct
    19261 ttgcaaccta acgtcgtcca tgcttttagc actaatgcct gcaaattggc ctcttgtaac
    19321 gcttccagac ctgcggggca gaaatactgt gtctcatcag agagatactt cgcctcataa
    19381 tcatagaagg ttccggccgg ttgaatacgt attgacggta aaatttcttc accgagtatc
    19441 gcaaccgtga actccggccc acttagccat ttttcaatca atacttcttc atcgtgctga
    19501 aatgccaatc ttaatgcatc ttgtagagca ttttctgcta ctacttttga cattcccaca
    19561 ctggaacctt cgcggctcgg cttaacgata accggcaaac ccagagaaga aatttctgct
    19621 aactgcttat cgttaaggcc tttttcaaac tctgcgcggg ttaacgctac ccacggcgcg
    19681 accggtaaac cggcaccctg ccacagaagt ttgctgcgta gtttatccat tgaaagcgca
    19741 gatgccatca ctccgctgcc ggtataaggc aagcccatca gctcgagcat gccctgcagc
    19801 gtaccatctt caccgccgcg accgtgtagt gcgataaaca ctttctgaaa acccatcgac
    19861 ttcagttgcg tcacgtcgac ttctttcggg tcgacaggat acgcgtcaat accgccttca
    19921 cgcagcccgg ctaacaccgc tgcgccagaa ttcagagaaa cttcccgctc agcggaggtc
    19981 ccgcccaaca ggaccgcgat tttatcagtc atgttgttct tcctccggag tttgcggctt
    20041 cagtttgatt tcagctaaag aacgagcaat ttttccgata ttaccagccc cctgaacgag
    20101 aatcaggtcg ttaccggtta ataccggtgc cagcatctcg gctacctgcg ccgggtccgg
    20161 caccagaatg ggatcaattt tcccacgtcc acgaattgtg cgacacagcg aacggctgtc
    20221 cgctcccgga attggcgctt caccagccgg atacacttcc agcatcaaca gggtatcaac
    20281 ctgcgtcagc acattggcga aatcatcata caggtcgcgc gtacgggtaa aacggtgcgg
    20341 ctgaaacagc attaccaggt ttttatccgg ccagcctgcg cgcgccgctt taatagtggc
    20401 gtccacttcc gtcgggtggt ggccgtagtc atcgaccagc attgccgtac cgctttttcc
    20461 attcactggc tccagcggga attcaccgag gaaatcgaaa cggcgccccg tcccctggaa
    20521 gctttccagc gcacgcaaaa tagcctcgtc gtcaattccc tcttccgtag caaccgcaac
    20581 cgcagctgcg gcgttcagcg cgttatgacg acctggcgca ttcagggtga cgcgcatcgg
    20641 ctctttgtcc tggcgcagca gcgtaaagtg cccctgcggg ccaatctgct gataatcttc
    20701 tacacgcacg tcggcatctt cgctgaagcc gtaagtcgtg gtctgacgtc ccacacgcgg
    20761 taacaattcg cggatcaccg gatcatcaac acacatcacc gcacgaccgt aaaacggcag
    20821 gttgtgcaga aaattaataa aagtctgttt taaattctca aagtctccct ggtaggtatc
    20881 catgtggtcg gcttcgatat tggtgacaat cgccaccatc ggttgcagat gcaggaacga
    20941 tgcatcactc tcatctgctt cggcaatcag gtaccgacca tgccccaaac gcgcatgaac
    21001 ccccgccgct tttaccagcc cgccgttaac gaaggttggg tcgagccccg cttctgcgta
    21061 gatgctggaa accatcgcgg tggttgtcgt tttgccgtgt gttccggcaa tggcgatgcc
    21121 atgacgaaaa cgcattaact cagccagcat ttcagcacga cggatcaccg gaatacgcgc
    21181 ttcatgagct gcgacaattt ccgggttatc ggcagaaatc gcgctggaaa caacgaccac
    21241 gctggcatca cgtacgtttt ccgggcgatg gttgaaataa atcgtcgctc ccagattcat
    21301 taactgctgc gtgaccggat ttggcgctaa atcggaacca ctgatctgat aaccttcatt
    21361 ggccagaact tcggcaatac cgcccatacc ggcaccaccg atgccgacaa aatgtatgtg
    21421 ccgaacgcga cgcatttcgg gcacgatgga acgcagtttt gccaattgtt gtgtattcat
    21481 tctttacgcc attaacttct taaattcata cgatgcaaaa ggcatcgcta caattacgcc
    21541 cgggcagccc ggctcacttc atttgccact cgctcggtgg catccggaat ggatgcagcg
    21601 cgggcgcgtt ctgccatggt taataaggtt tctcgcgacc acccggccag ggtgttggcg
    21661 acagcatcca cgctaagctg tgactgctcg ataattttgg cagcgcccgc tttttccagc
    21721 ggtagcgcat tccagtattg ctgacggtct ttatgttgaa acggcacaaa caacgccggt
    21781 agtcctgccg cggcgatttc actcaccgtt aacgcaccgg agcggcaaac gacgacatcc
    21841 gcccacgcat acgccgccgc catatcatca ataaattccg tcactttatg ctgcggttgc
    21901 cccgcttcgg catacgcctg ttcaacggat tgttgcgaac ctttgccgct ctgatgccag
    21961 atagtgactg aatcacccag tttcgcagca acctgcggca ttgtctggtt aagaatgcgt
    22021 gcgccctggg aaccccccac taccaacaca cgaaccggac cttcacgtcc agccaaacgt
    22081 tgctgcggca acggcagcgc cagcacatcg gtacgcaccg ggttacccac cacttccgca
    22141 ttaggaaaag cacctggaaa cgcctgcatc actttggtag caatcttcgc cagccattta
    22201 ttggttaagc ccgcaatacc gttttgttca tgaagtacaa ccggaatgcc taacgaccac
    22261 gcggccagac cacctggacc tgatacatag ccgcccatac cgagcaccac gtcaggtttg
    22321 tacgctttca taatcgcccg cgcctgacgc caggcgttga agatacgcag cggcgcagct
    22381 atcagtgctt ttataccttt tccacgcaga ccagagatac gaatgaaatc aatttcgatg
    22441 ccatgttttg gcactaagtc cgcttccata cggtcggcag tccccagcca gcgaacttgc
    22501 caaccctgag ccattagatg gtgcgcaacc gccagccccg ggaatacatg tccaccggtt
    22561 ccgcctgcca tcaccattaa tcgcttccct tgaccactca tcgtgaacct cgtacaaacg
    22621 cctgcgcttt ctccagacgc gtttcataat caatacgcaa cagcatcatg atggctgtcg
    22681 acataatcag taagctcgaa ccaccgtaac tgatcagcgg caatgtcaga cctttggtcg
    22741 gtaacatccc cgccgccgcg cctacgttaa ccagcgcctg gaaactaaac cagataccaa
    22801 tagaacaggc gagaaaaccg gaaaaacggt ggtcaatttc taatgctttg cggccaatcg
    22861 acatcgcgcg aaaagcgacg aagaatacca ttaaaagtgc cagcaccaca ccgacatacc
    22921 ccagttcttc gccgataatg gcgaaaataa agtcagtgtg cgcttccggc agatactcca
    22981 gtttttgtac cgagttacct aaaccttgtc cccacagttc accgcgacca aacgccatca
    23041 acgattgcgt taactgatag ccgctgccaa agggatcttc ccacgggttc cagaatgcgg
    23101 taacacggcg gatacggtac ggttcggcga ggatcagcaa cacaaccgct gaaatgccca
    23161 taccgataat ggcaatgaac tgccacaatt tcgctccagc caggaacaac atcgccagcg
    23221 tagtcacaaa caacaccacc accgtaccaa ggtctggctg tgccagcagt aacactgcca
    23281 acaccagaat tacgcccatc ggtttcagga agccgcgcag gttattacgt acttcgtcgc
    23341 ctttacgcac cagatagttg gcgatatagc aaaacagcga cagttttgtc agctccgcag
    23401 gctgaatacg cagcaaaccg agatcgatcc aacgcgatgc ccctttaacc gagctaccca
    23461 ctaccagaac gatcatcagc aggatgatag atccgagcag catcgtggca ctgtagcgtt
    23521 gccagaactc catcggcaga cgcagcgtaa tgatcgccag aatgaatgcc aggatcagat
    23581 agacgccatc acgcttcgcg aagaagaacg gatcgttggt taagcgttgc cctatgggca
    23641 ttgacgccga ggtcaccatg ataaagccaa tcgccgcgag gccgaaggtc agccacagta
    23701 aggtgcgatc gtacatgatc aggctgtcgg tatctttttc ccgcgagccc atcacccagc
    23761 cctttagcgc cgtggagatc cagaccagaa tactgaatcc tggcaggcgc ggcattttca
    23821 ggcgagggag agataaacgc atcaacctaa ctccttcgcc agacgggcaa actcattgcc
    23881 tcgttgttca aagttcttga attgatcaag gctggcacag gccggggaga gcagaaccat
    23941 atcgcccggc tgaacacgcg tagcaagcaa gcgcatcgcc tgttccatag tttcggtttg
    24001 ttctgccact tccgggcgta gcgccgccag ctgcgcgccg tcccgaccga aacaatacag
    24061 acgtacgtta tcgccattca ggtaacgcgc cagtgggcta aagtccgccg atttaccatc
    24121 gccccccagc aacaaatgca gcgtgccgtc tacgtgcagg ccattcagcg ccgcttccgt
    24181 actgccgacg ttggtcgctt ttgaatcgtt aacccagcgc acaccgttat gctccagcac
    24241 aacttcaaag cgatgcggca gaccagtgaa tgtggttaac gctttcaggc tgctggcacg
    24301 cggtaaccct gcggcatctg ccagcgccag cgccgccagc gcattggtgt agttatgctg
    24361 cccggaaagt ttcatttctt tcacgttcag cactttctcg cctttcaccc gcagccaggt
    24421 ttcaccctgc tgatgattca ggtgatagtc gcccatgttg acgccaaagc tgacgcagcg
    24481 ttcatccgca ccgcgaatcg gcattgttaa ggcatcatca gcattaacca cgcaaacttt
    24541 tgcgttttcg taaatgcgca gttttgctgc acgatactgt tgtaaaccaa acggatagcg
    24601 atccatatga tcttcagtca cgttcagaat ggtcgctgcc accgcttgta agctggaagt
    24661 ggtttccagc tggaagctcg acagttccag cacgtacagt tcgcactcat catccagcag
    24721 catcaacgca ggcaggccaa tattgccacc cacaccaacg ttaacccccg ccgctttcgc
    24781 catttcacct actagcgtgg tgaccgtgct tttgccgtta gaaccggtga tcgccacaat
    24841 cggtgcttgt gcttcacgac agaacagctc gatatcgcca acgatttcga ttccggcatc
    24901 agcggcagcg cttaaggatg gatgcgccag tgcaataccg ggactggcga caatcagatc
    24961 tgccgccatc agccattcat cattcagacc gcccgtgtgg cgttctacgg cttcgggtaa
    25021 tttatccagg ccaggcggtg tcatacgcgt atccataacg cgcggcgtca caccgcgagc
    25081 gaggaaaaag tccacgcagg aaagcccggt gaggcccagg ccgataatga cgacattttt
    25141 accctggtaa tcagccatga ttaacgtacc ttcagcgttg ccagaccaat caggaccagc
    25201 atcagcgaaa taatccagaa acgcacaatg acgcgcggtt ccggccagcc tttcagttca
    25261 tagtggtgat gaatcggcgc catgcggaaa atacgttgtc cgcgcagttt aaaggagccg
    25321 acctgcagga tgacggaaag cgtttctacc acgaacacgc cccccataat caccagcagg
    25381 aattcctgac gcagcagtac ggcgataata cctaacgcac cacctaacgc cagcgaacct
    25441 acatcgccca taaagacctg cgccggatag gtgttaaacc acaggaagcc cagccctgcc
    25501 ccgactatcg cggtacagac aatgaccagt tccccggcgt gtcgtagata cggtatgtgt
    25561 aaatagctgg caaagttcat gttgccggtc gcccacgcca ccagcgcaaa accaccggcg
    25621 acaaatacgg tcggcataat tgccaaaccg tcgagaccat cggtcaggtt taccgcgttg
    25681 ccagtaccca caatgacaaa gtaagccagc agaatgtaga acagccccag ttgcggcatc
    25741 acatctttaa agaacggaac caccagctgc gttgcgggcg tgtctttgcc gacaaggtac
    25801 agcgcgaagg cgacacccag cgcaatcacc gacatccaga aatacttcca acgggcaatc
    25861 aggcccttgg tgtctttacg caccactttg cgataatcat caacaaagcc aataacaccg
    25921 taacctacca gcaccaccag tacgcaccag acgtacggat tggacgggta agcccacagc
    25981 agcacggaga tcacaatcgc cgtcaggatc attatcccgc ccatggtcgg tgtaccgcgc
    26041 ttgctgaaat gtgattcagg accgtcgtta cgcaccacct gaccaaagga aagtttttgc
    26101 aaatgagcaa tcatacgcgg gcccatccac aatgagatga acagcgcggt cagcaggctg
    26161 acgatggcgc gaaacgtcag ataggaaaag acgttaaagc cggaataata tttgaccaaa
    26221 tgttcggcca gccaaactaa catgtcccat tctcctgtaa agcgcgtact acctcttcca
    26281 tggcagcact acgtgaaccc ttaactaaaa tcgtaattac ctgttgctca gcaatcagtg
    26341 atttaagacg cgcgataagc gcagttttat cggaaaaatg ttcgccaacg ccgctggcgg
    26401 tgctgatagc atggctttgt ttacccacac ttaacacgca gtcaatacca gcagcttttg
    26461 ccgcttcgcc cacctgtaca tggcaggctt cgctttcagc gcccagttcc gccatatcgc
    26521 ccaccaccag cacgcggtag cccggcattt cagccagaac ctgaacagcg gcggtcattg
    26581 aaccgacatt ggcgttgtag gagtcgtcga gcagcaactg gttttctgcc agttttatgg
    26641 ggaacagacg gcctggaaca gctttcagat ttgccagccc cgctttgata gcatcaagcg
    26701 ttgcgcccac ggacatggag agcgcagcgg ctgccagcgc attcgcaata ttgtgacgcc
    26761 ccggcaacgg cagcagaaca tcgacgctac ctgttggggt ttgcagggta aattccgtac
    26821 cgtgcgaggt tacatggata ttggtggcgg tgaaatcgct gttggcggca ttaggtgaga
    26881 aacgccacac tttgcgtgag ccaattacgc tctgccagtt caaccagtcg ttgttgtcgg
    26941 cgttcataat ggcgataccg ttttccggca ggccgctaaa gatttcacct ttcgctttcg
    27001 cgacacccgc aagcgagcca aaaccttcca gatgcgccgc tgccaggttg ttgaccagcg
    27061 ccgcttccgg gcgagtcaga ctcacagtcc aggcaatttc accctgatgg ttcgcgccaa
    27121 gttcaataac tgcgtaatcg tattccggcg ttaagcgcaa cagcgtcatc ggtacaccga
    27181 tgtcgttatt gagattgcct gccgtataaa gtgtgttgcc gcactggctt aaaattgccg
    27241 ccgtcatctc tttaacggag gttttgccgg aggaacctgt cagagcaacc acgcgcgccg
    27301 gaacttgctg gcgaacccat gcagccagtt caccaaacgc cagacgcgta ttcttgacga
    27361 ttaactgcgg cagatcaata tccagcggac ggctaaccag tagtgcgcct gcgccgccag
    27421 ctttcgcctg gtcggcaaaa tcatgagcat cgaaacgttc gcctttcagg gcaacaaaca
    27481 ggcagcccgg cgtcagtttt cgcgtgtcag tggttacagc atcaagggta atatctgcac
    27541 cttgcagttc accgttgaga atatcggtaa gttggctaag ggttacgcta atcatgcaat
    27601 cccccccagc agacgcgcca ccgtgacgcg atcggagtag tccagacgct gattgccaac
    27661 aatctggtaa tcctcatggc ctttgcccgc aaccagtacc acatcattct ctttagcctg
    27721 cataacggcg caagtcaccg cttcagcacg gccttccatc actttggcat atccggcatc
    27781 taacattccc gccagaatat cgttgatgat ggcacgaggc tcttcggtac gcgggttatc
    27841 gtccgtcacc accgccacgt cagcaaactc ttcagcaatt gcgcccatca gtggacgttt
    27901 gcctttatca cgatcgccac cgcagccaaa gacgcaccac agcttgcccg cacagtgcag
    27961 gcgcgccgcc tgtaaggctt tttccagtgc atccggcgta tgcgcgtaat ccaccaccac
    28021 cgtcggtttg cctggtgcag tgaacacttc catacgtccg caaaccggtt gcagacgcgc
    28081 ggcggttttc agcaaatcag ccagcggata gccgagtgcc agcagtgtcg ccagcgccag
    28141 cagcaggttg ctgacgttga atgcgcccat caggcgactt tcaatttccc catcgcccca
    28201 actcgagcta aagcgaatgg tcgcaccgct gtcgtgatag ttcacgtcgg tcgctttcaa
    28261 ccagcgtccg tgacagttcg gattgatatg atcttccatt gataccgcaa ccgcgtccgg
    28321 cagttttgcc agccagcggc ggcccacttc atcgtcggcg ttaataatcg cctgaccgca
    28381 atgatgtgca gaataaagca gccatttcgc ggcttcgtag tgttccatat caccatgata
    28441 atcaaggtga tctcggctta agttggtaaa gaccgacgcc gcaaatttca atgccgccac
    28501 acggtgttgt accagcccgt gggatgaaac ttccattgcg caaaaagtcg cgccctgatc
    28561 caccagcccc gccagctcat gctgaacatc gactgccgaa ccggttgtat tttctgtcgg
    28621 gatcactttc cccagcaggc cgttaccaac ggtgcccatt accgcgctgg tttcgccaag
    28681 cagttggctc cactgcgcca acagctgggt agtcgtggtt ttgccgttgg tgcccgttac
    28741 gcccacgaga cgtaaattgt cagagggttc atggtaaaag cggcccgcca gtgcagataa
    28801 acgctcgttg agctggctga gatagatgac cggtacgccg tgcatttcac ggatttcacc
    28861 gtcggtcgcc tcatctttcg cctctgcaat aatggcagcc acaccttgcg ctatcgcctg
    28921 cgggatatat cgacgcccgt ccgcctgatg acctactaca gctacaaaga gatcgcccgc
    28981 cgcagccaca cggctgtcga gtgtcatctc tcgcagtgct cgcgaaggtg cgtctggcac
    29041 ccacggagca agaaggtcgc gcaaattacg atctgccacc tgtaccctcg ccttgattaa
    29101 tcacaaattc atttttatcg cccgttgtca gcgcatccgg ctcgatgttc atggtacgca
    29161 atacgccgcc catgatggca ccaaagaccg gcgcggaaac ggcgccgccg tagtatttac
    29221 ccgcctgcgg atcgttgata acaacaacca gcgcgaagcg cggctgactc gcaggcgcaa
    29281 cgcctgcggt ataagcaata tatttattga tgtagcgacc gtccggcccg acctttttcg
    29341 cggtaccggt tttaatggcg atacgatagc ctttaatcgc cgccttcacg ccgccgccgc
    29401 ccggtagcgc cacgctttcc atcatatgca ccacggtgcg aacaatggat tccgggaaga
    29461 cacgttcacc gggaaccggg gggtcaactt tggtaatcga cagtgggcga taaatgccgt
    29521 agctgccgat agttgcgtag actcgcgcta actgtaacgg tgttaccatt agcccgtagc
    29581 cgaaagagaa ggtggccctc tctatgtcag accaccgttg tttttgagga tataagccac
    29641 tgcgttctcc gaccaacccc aaattggtcg cttttcccag tccaaaacgt gagtaagtat
    29701 ctactaacgc tgaggacggc atcgctaacg ccagtttgga aacaccgacg ttactcgact
    29761 tctgtaatac cccggtcagg gttaattcgc tgtagcgcgc cacgtctttg atttcgtgac
    29821 cgttaattcg ataaggaacg gtattgagta ccgagttttc ccgcaccacg ccacgttgca
    29881 acgcggtcat caccaccatc ggtttaaccg ttgagcccgg ttcaaacacg tcggtgatgg
    29941 tacggttacg catcgcttct ttcggcgtgc cgctcagatt atttgggttg tatgacgggc
    30001 tgttagccat cgccagcact tcaccggtgt tgacatccac cagcacggcg ctaccagatt
    30061 cagccttgtt aaaggctacc gcgttgttca gttcgcgata aaccagcgcc tgcaggcgtt
    30121 catcaatact cagcgtcagg ttgtgcgctg cctggctgtc agtagaagaa atatcttcaa
    30181 ttacgcgacc atagcggtct ttacgcacaa tgcgctcacc cggctgcccg gtaagccatt
    30241 tatcgaaact cttctcgaca ccctcaatcc cttggctatc gacgttagta aagccgatga
    30301 ggtgagcggt cacttcgccg gatggataat aacggcgaga ttcttcacgc agatgaatcc
    30361 ccggcagttt cagttttttg atgtactctg ccatgtcagg gtttacctga cgcgccagat
    30421 aaataaagcg ccctttcggg ttggcgttaa tgcgggctga aagctgatcc agcggaatgt
    30481 tgagtgcgtt agccagcgcc ttccagcgat caccgacgct gataccgcca gcgtcatgca
    30541 cttctttcgg gtcagcccaa atcgctttaa ccggcacgct caccgctaac gggcgaccag
    30601 aacggtcagt aatcatgccg cgggaggtgg aaacttgctg aacgcgaaga gaacgcatgt
    30661 cgccctcttt caccagcata tccggggaga taacttgtaa ccacgctacg cgtccgagca
    30721 gaaaagccag cgccaggaga atacagccgc ataacaacgc aaaacgccaa ctgataaagt
    30781 tggcatgttc ttcctgacgt tttggtttct gcgttttcgc cgctgctttc atgcgtcgcg
    30841 tttatcctta tttttgcact acgatatttt cttgtgacgg atcaacatgc tgcatttgca
    30901 gcttttccgt ggctattctt tccacccggc tatggtcgcc gagcgcattc tcttcaagga
    30961 tcaggttgcg ccattcaatg tctaaagcat ctcgctccag caccagttgt tcgcgctgag
    31021 cggtcagtaa acgggtatgg tgcgccgtgg ttaccacggt caccgccgtc aaaataatgc
    31081 aaatgaacag gcagagtggc agcttcccaa atcgcaaaag atcgtcaccg ataacgccag
    31141 gcaaggcatg gcgctcgtgg cttcccatcg atcctttaac tttgcttaga gcttctgtca
    31201 ctctgctgat catgcgttcg tcctctctgc aatacgcaga actgaactac gggcacgagg
    31261 gttctcagcc acctcttctt cgcccggcat caacttacct agtgctcgca actgacgccc
    31321 ccccagtttt ttgagctgct cttctgtcat cggtaaccct gccggaactt gcggaccgcg
    31381 gctgttttca cgcataaaac gtttcacaat gcggtcttca agtgagtgga agctgatgat
    31441 cgaaagccgc ccacccgggg ccagcacatg gagcgagctt tttagcgcct gctctatctc
    31501 ctccagttca ctgtttaccc aaatgcgcac cgcctggaag gtacgggtcg cgggatgttt
    31561 aaacttatct ttcaccggcg ttgcagcagc cacgacttcc gccagttctt tggtgcgggt
    31621 catcggctgt tcgcggttac gctcgacaat ggcgcgggca atgcgtttgg caaaacgctc
    31681 ttcaccatag gttttcaata cccaggcgat atcggcttct tctgcggttt gtagccattc
    31741 agcggctgac tgcccacggg ttgggtccat acgcatgtcc agcggaccat cgcgcataaa
    31801 ggaaaagccg cgctcagcat catcaagttg cggtgaagag acgccaagat cgaggagaat
    31861 gccgtcgatc ttgccgataa gatcgcgctc ggcaacatat tcgcccagcg cggagaaagg
    31921 tccgtggatg atggagaagc gcggatcatc aatagtcttc gcaacggcga tagcctgcgg
    31981 gtcgcgatcg atcgccagca aacgcccctc ttcgccaagc tgcgagagga tcagacgtga
    32041 gtgaccaccg cgaccaaaag tcccatcaat gtagatgcca tcaggacgga tattgaggcc
    32101 attaacggct tcatccagca gcaccgtagt atgtttatag ttttccatca ttttatagag
    32161 acaagtcctg cagtcgctcc gataagtctc cggtagccaa ctgctctgcg tcgatatctt
    32221 ccttgacctg ttgatgccag gttgtttcat cccacagctc aaacttgttg aactgtccaa
    32281 ccagcatcac ttcttttgtc agcccggcat gttgccgcag tactggcgcg attaacaatc
    32341 gacctgcgcc atccatctga cattcgctgg catgacctaa cagtaggcgc tgcacacggc
    32401 gctcaaccgg gttcatgctc gacagacgcg ataatttttg ctcgataatt tcccattcag
    32461 gcagggggta aagcagcagg cacgggtgat gaatgtcaat ggtgcaaacc atttgaccgg
    32521 cagcattctc aagcagctgt tcccgataac gggtaggcac tgataagcgc cctttgctgt
    32581 cgagattgac taacgttgct ccccggaaca tgccagcctc accccttatt cccactttgc
    32641 cccacaattt cccactgaaa ggagtttacg gagcggagga aaagcttgtc aagccagtca
    32701 caaggcatac cgaggtaaaa aacccgatgt ttacggctaa tatcagcgtt gtttatttta
    32761 cgtccaacgt gcgaagcaaa ttaacgttat gaatagctgt aagaaaaaat ccctcatcgt
    32821 catcaccgtt ttaaaatgac tcactatttt ccacagcaaa tataaagtgt cagtttgcga
    32881 cgcgagcggc attttaggac atatcttctc tggttaacag tccctgttgc gtctgtgtgg
    32941 cgcttgatgc cagcaggttt gcgcaaaaac aaagaaaagt gtttgttaaa tcggcaattc
    33001 atctctgcat gtaacaaaat aatgcaaaaa cgcgctgtca atatcattta attattgtga
    33061 atttcagcag gtgagttaat taacgggcgg gtttaaaagc atttatcaat aatagccagg
    33121 aatagtctta tttactttaa ggatattgat gtccattccc gtactcaacg cgccagagag
    33181 aaattcacct ggcgcgtact tttgttcgcg gcttagctac ggctgagcac gccgcggcga
    33241 tagagattac gtttaatgcg cgttaaacca ggttttggct tacgcggttc gtccaggctc
    33301 gccaggacaa tctccagcac acgttctgca acatcgcggt gacgttgagc cactgccagc
    33361 accgggcact gtaagaagtc gagcagttcg ttatcgccaa aggtggcaat tgccaggtca
    33421 gaaggcagtt tgccgtcgcg acgtagcgtg acatccatca ccccttgcaa caacgcaaac
    33481 gacgttgtga acagcgcctg cggcatcgga tgcgtttcca gccatttttc gaataactgg
    33541 gcagctgcct cccgctcata gctgttggca taaaggaaat gcacttcgcg cggatcatct
    33601 ttccaggcag tacggaagcc ttgttcacgc aggaagctga cagaaagctc cggcagcgcg
    33661 ccaagataaa gcaccgtctc ggcgggaaac ttacgtaact cttccgccag catttcggca
    33721 tcatcctgat cggcaccaac cacgctggtg aagtgttcac gatcgagggc gcggtccagc
    33781 gcgacaatcg ggaacgggtc gttagcccag cgttgataaa aaggatgctc aggtggcaac
    33841 gacgtcgaaa caataatggc atcaacctga cgctgtaaaa ggtgctcaat gcagcgcatt
    33901 tcgttgtcgg gctgatcttc cgagcaggca atcagcagtt gataaccccg ttgccgcgcc
    33961 tggcgttcaa gatagttagc gatgcgggta tagctggtgt tctccagatc ggggatcaca
    34021 agaccaatag aacgtgtgcg tccagcacga agcccagctg ccacggcgtt cgggtgataa
    34081 ttgtgctcac gcactactgc catgactttt tcaacggttt tgtcgctcac acggtattgc
    34141 ttcgctttgc cgttaataac atagcttgca gtggtccgcg acactcccgc cagccgagcg
    34201 atttcatcca gtttcacaat tgccccttgc gtaaaatgta aaaaccatag ccattgtact
    34261 ggtatgggtt aaattcatta acatctaagc gcagaaaatt aactgcggca actgctttta
    34321 ttccaggttc ccactgattt cgcaaaaaaa gcccaacgtg acatgttggg ctgcaaattg
    34381 cgcattgaga tcattctcaa cacattattt tatcgccgcg tgaaagtccg accacaccag
    34441 agcgagcaac ttccacaatt ttcgccacat cgcgaatcga tgctaaaaat gcatcaagct
    34501 tatcgctggt gcctgctaat tgaacggtat aaagcgaggg tgtgacatcg ataatttgcc
    34561 cacggaatat ttccgtatta cgtttcactt cgtcacgccc gtaaccgctg gcctgaattt
    34621 tcaccagcat gatttcccgc tcaacgtgcg cgccctgccc caactcgctc acgcgcaaga
    34681 catcgaccag cttgtgtaat tgcttttcga tttgctcaag cactttttca tcgcccacgg
    34741 tctgaatggt catacgcgat aatgtcggat cgtcggttgg cgcaacggtc aggctttcaa
    34801 tgttgtagcc acgctgggaa aaaaggccaa tcacgcggga taacgcgcct gattcatttt
    34861 cgagtaagac tgataatatc cggcgcataa tcaggttctc tccgttttgc ttaaccacat
    34921 ttcatccatt ccgcccccgc gaatctgcat cgggtagacg tgctcactgc catcgacggt
    34981 aacatcaaca aacaccaggc gattattgcg cacctgttcc agcgcctcgc taagtttgct
    35041 ttccagttct tgcggatgag agatctggat cccgacgtga ccataggctt ccgccagacg
    35101 gacgaagtct ggtaaggact gcatataaga ttgtgaatga cggccggaat agatcatgtc
    35161 ctgccactgc ttcaccatcc ccagatagcg gttattgaga ttcaccacca gtacgggcaa
    35221 ctcatattgc aacgcggtag acagttcctg gatgttcatc tgaatactgc cgtcaccagt
    35281 gacgcaaacc acggtttctt ctggcaacgc cattttgacg cccagtgccg caggtaaacc
    35341 aaaacccatc gtgccgaggc cgccagagtt gatccagcga cgcggtttgt cgaatggata
    35401 ataaagcgca gcaaacatct ggtgctgccc gacatcggac gtcacgtaag cgtccccatt
    35461 agtcaaccgc caaagagtct cgatcaccgc ctgcggttta atcttttcac tgtgagtgtc
    35521 atatttcagg cactgacgag tgcgccactg ttcaatctgc tgccaccagt cgcggatctc
    35581 atccagcggt tgatgggcgg attcttgcga caggagttca agcatttgtt ctaggacctg
    35641 gcgagcatcc cccacaatcg ggatatcagc agtcacggtt ttagaaattg aggttggatc
    35701 aatatcgata tgcagaacag tggcatttgg gcagtacttt gccagattgt tcgtcgttcg
    35761 gtcatcaaaa cgtaccccga cggcgaaaat cacatccgcg ttatgcatcg tcatattggc
    35821 ttcgtaggta ccgtgcattc ccaacatgcc cagtgcctga cgatgcgttg ccggaaacgc
    35881 ccccagcccc atcaaagagg aaacaacggg cagattcaac gcctccaccg cttctttcaa
    35941 ctgcagatgg cagcccgccg tgattgcccc accgcctacg tagacaaccg gtttttttgc
    36001 cgccaccagc gtttgcagag cgcgcttaat ttgcccttta tgcccggagg tcgtgggatt
    36061 gtaagaacgc atactgaccg actccggcca gacatagggt aatttgttcg ccggatttag
    36121 aatatctttc ggtaaatcaa cgaccacggg tccaggacga ccacttgccg ctagccagaa
    36181 agcctttttc agcacctgcg gaatgtcttc cgtttgctta accagaaaac tgtgtttaac
    36241 caccgggcga gaaatcccca ccatgtcgca ctcctgaaag gcatcataac ctatcaacga
    36301 ggtcgctacc tgcccggaaa ggacaactaa tggaatggaa tccatataag cggtggcaat
    36361 gccagtaatt gcattggtcg cccctggacc cgacgttacc agcacgacgc cgacttcccc
    36421 ggtcgcgcgc gccagaccat cggccatatg caccgccgcc tgctcatggc gaactaaaac
    36481 atgatcgata ccacccacgg tatgcaatgc atcgtaaata tcaaggaccg cgcctccagg
    36541 ataaccgaat acttgtttaa cgccctgatc gataagcgat cggacgacca tctcggctcc
    36601 agacaacatc tccatggcct gcctcactgt ttgacggaaa aaatgtgtaa aacacattaa
    36661 ccgtccgatc ttgcttaagc aattggcaga cagagacata aacacggggt aataagaata
    36721 aacaggagga aaggtgaaaa gaaacgataa aatcctccat tactgaggat gaaattgata
    36781 ataaatcggc tgaatcctac aacttacagc aatttctcta ttttttta
//
LOCUS       DAZGJT010000041        35099 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZGJT010000041 DAZGJT010000000
VERSION     DAZGJT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.041"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            150..740
                     /locus_tag="ACHKFK_002223"
     CDS             150..740
                     /locus_tag="ACHKFK_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9052559.1"
                     /translation="MSKKLGFALSGIMLAMAAGTAFAANDMDGGQLNISGLVVDNTCE
                     ARVDGGNKDGLILLQTATVAEITDGVLDTTVGAKAKPFSITIDCSKANPAPGTTAKMT
                     FGSVFFGNSKGTLNNDMSINTPSDGVNTALHNIEGSTIKQVQVNNPGDVYSKTLDSTS
                     KSATYDFKASYVRADASKAATAGYVKTNSAYTITYQ"
     gene            849..1589
                     /locus_tag="ACHKFK_002224"
     CDS             849..1589
                     /locus_tag="ACHKFK_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO9052560.1"
                     /translation="MFFNTKHTAALCLASCMAFSSSAIADIVISGTRVVYKSEQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTASS
                     ALPKDRESVFWFNVLEVPPKPDAAKAANQSLLQLAFRTRIKLFYRPEGLSGISSDAPL
                     ALKWSWATSEGKAALRVDNPTPYYVSFSSGDLEANGKRYPLDMTMIAPFSNDVIKVNG
                     MSGRTSSAKVHFFAINDFGGSIEGNANL"
     gene            1624..4212
                     /gene="htrE"
                     /locus_tag="ACHKFK_002225"
     CDS             1624..4212
                     /gene="htrE"
                     /locus_tag="ACHKFK_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein"
                     /protein_id="HFO9052561.1"
                     /translation="MTIKSTNHLTHIATFCALLYSNSALCAELVEYDHTFLMGKDASN
                     IDLSRYTEGNPTLPSIYDVSVYVNDQPIMSQSIAFAVIEGKKNAQACITQKNLLQFHI
                     SSPDKNSEKAILLKRDDDLGDCLNLAEMIPQSSIRYDVNDQRLDIDVPQAWIMKNYQN
                     YVDPSLWENGINAAMLSYNLNGYHSESPVRTNDSIYAAFNGGINLGAWRLRASGNYNW
                     MTNVHSDYDFQNRYLQRDLASLRSQLVIGESYTTGETFDSVSIRGIRLYSDSRMLPPV
                     LASFAPIIHGVANTNAKVTVMQNGYKIYETTVPPGAFAIDDLSPSGYGSDLIVTIEEA
                     DGTKRTFSQPFSSVVQMLRPGVGRWDISAGQVLKDSIQDEPNLFQASYYYGLNNYLTG
                     YTGIQLTDNNYTAGLLGLGMNTPVGAFSVDVTHSNVSIPDDKTYQGQSYRISWNKLFE
                     NTSTSLNIAAYRYSTQHYLGLNDALTLIDEVEHPEQDLEPKSMRNYSRMKNQVTVSIN
                     QPLKFEKKDYGSFYLSGSWSDYWASGQNSTNYSIGYSNSASWGSYSISAQRSLNEDGQ
                     TDDSIYLSFTIPIENLLGTEHRSSGFQSIDTQLNSDFKGNNQLNISSSGYSDTNRISY
                     SVNTGYMMNKSSDDLSYIGGYASYESPWGTLSGSASASSDNSRQFSLNTDGGFVLHSG
                     GLTFSNDSFSDSDTLAVIQAPGAKGARINYGNSTVDRWGYGVTSALSPYHENRIALDI
                     NDLENDVELKSTSTVAVPRQGAVVFADFETVQGQSAIMNIVRSDGKNIPFAADIYDEQ
                     NNIIGNVGQGGQAFVRGIGQEGNIRITWIEEGKPVSCFAHYQQNTTSEKIAQSIILNG
                     LRCQIQ"
     gene            4229..4795
                     /locus_tag="ACHKFK_002226"
     CDS             4229..4795
                     /locus_tag="ACHKFK_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9052562.1"
                     /translation="MIKITPHKITILMGLLLSPSVFATDVNVDFTATVKATTCNITLT
                     GTNVTDNGKDKYTLVIPSMGMDKIANKTAQSEANFKLVANGCSSGISWIDTTLTGNQS
                     GSSPALIIPLASDTTSTTSYIGMGFKRKATSGDTFLKPNSAEYIRWSASEISTDGLEM
                     TVALRETSVGKGVPGKFRALATFNFSYQ"
     gene            4807..5415
                     /gene="yadL"
                     /locus_tag="ACHKFK_002227"
     CDS             4807..5415
                     /gene="yadL"
                     /locus_tag="ACHKFK_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020235143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO9052563.1"
                     /translation="MMILKKLCYGLSGSLFLASSLLTISSHAATDSIGLTVTTDVEMG
                     TCTSTLTDDGTTKISTINFGDVYISEINAETKIKTFKLQFKDCAGIPGKKAKIKLTTR
                     ALCEGNSNNGPGFANASTATAKAAAVAVEVWSTSTPGKNGAKQFSCVTPATEEVSIAN
                     ATGSNVVDYPMSAVLVVAKDKTVTNVTAGDFTAPATFTVTYN"
     gene            5442..6038
                     /locus_tag="ACHKFK_002228"
     CDS             5442..6038
                     /locus_tag="ACHKFK_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO9052564.1"
                     /translation="MRPTQRNLMKKSILILSLLFSATSPVVAGQDVDIVANIKNNTCQ
                     SGISNNGNIDLGVVGVGDFTGNISAENYHPGGKEFTITVQDCTLQGTGNVLNQLHINF
                     RALSGVMATGSSQIFANEDNAGAKNVGVVIFSIQDPANTFNVLSATGSSRSVYPVMSS
                     ALNNSSWKFSTRMQKIDPALNVTSGPLISHVLVDIYYE"
     gene            6064..7347
                     /locus_tag="ACHKFK_002229"
     CDS             6064..7347
                     /locus_tag="ACHKFK_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO9052565.1"
                     /translation="MQHDNGVKMKTFFRYFLFLALCSCCYTASAGTDDDVGYIVGNNY
                     GVGPGDQKWRETGPNGDVTVKFRYGSGTNNLVFYKPTQLGPTGVSLKWAQLDSASGGG
                     FLYCNRSDNSSGAPMSIEHKMVDSGKSYGGHKLFKTSVPGLYYTLAISNIWSTYTYTD
                     INPSGMYIGDSTSQSFNWRGESEQTLYWSCNNANSSKKYWAVGGVMQTLTIEFYTDTD
                     FNPTTNQRVTLSRTDSYLYSFKAYNAGISIKSYFLKIDFDLTDIVLTNPTCFTAALSG
                     PSVNGSTVKMGDYSPAQIKNSATAVPFDITLQNCIRVRNIETKLKSNKVGSVSKELLA
                     NTLTGNDAAKGVGVLIEGLKNTKSAQMVLKPNNATSIYKDYETEDDTTGGIYPDKGNG
                     TSQPLHFQATLKQDGNIAIEPGDFKATSTFQVTYP"
     gene            7459..8253
                     /gene="panB"
                     /locus_tag="ACHKFK_002230"
     CDS             7459..8253
                     /gene="panB"
                     /locus_tag="ACHKFK_002230"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO9052566.1"
                     /translation="MKPTTIASLQKCKQDKKHFATITAYDYSFAKLFADEGINVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8265..9116
                     /gene="panC"
                     /locus_tag="ACHKFK_002231"
     CDS             8265..9116
                     /gene="panC"
                     /locus_tag="ACHKFK_002231"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO9052567.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRSDDIQIRDADTLLEISENSKRAVILVAAWLGDARLID
                     NKLVELA"
     gene            complement(9198..10082)
                     /gene="rpnC"
                     /locus_tag="ACHKFK_002232"
     CDS             complement(9198..10082)
                     /gene="rpnC"
                     /locus_tag="ACHKFK_002232"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO9052568.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLDIHLPAELRELCDLDTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGESMMTLAQWFEEKGIQQGRQEVSQEFALRLLSKGMSRE
                     DVAEMVNLPLAEIDKMIN"
     gene            10356..10736
                     /gene="panD"
                     /locus_tag="ACHKFK_002233"
     CDS             10356..10736
                     /gene="panD"
                     /locus_tag="ACHKFK_002233"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO9052569.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10740..11969)
                     /gene="yadE"
                     /locus_tag="ACHKFK_002234"
     CDS             complement(10740..11969)
                     /gene="yadE"
                     /locus_tag="ACHKFK_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9052570.1"
                     /translation="MYKQAVILLLMLFTASVSATLPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRIDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVLYLSYPFGGFNDKAVKAAKEAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12033..12473)
                     /gene="yadI"
                     /locus_tag="ACHKFK_002235"
     CDS             complement(12033..12473)
                     /gene="yadI"
                     /locus_tag="ACHKFK_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9052571.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHETDSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSAFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12578..13348)
                     /gene="yadH"
                     /locus_tag="ACHKFK_002236"
     CDS             complement(12578..13348)
                     /gene="yadH"
                     /locus_tag="ACHKFK_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9052572.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13345..14271)
                     /gene="yadG"
                     /locus_tag="ACHKFK_002237"
     CDS             complement(13345..14271)
                     /gene="yadG"
                     /locus_tag="ACHKFK_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9052573.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFFLDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRT"
     gene            14380..15042
                     /gene="can"
                     /locus_tag="ACHKFK_002238"
     CDS             14380..15042
                     /gene="can"
                     /locus_tag="ACHKFK_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO9052574.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTGTNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15083..15619)
                     /gene="hpt"
                     /locus_tag="ACHKFK_002239"
     CDS             complement(15083..15619)
                     /gene="hpt"
                     /locus_tag="ACHKFK_002239"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO9052575.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15825..18215
                     /gene="gcd"
                     /locus_tag="ACHKFK_002240"
     CDS             15825..18215
                     /gene="gcd"
                     /locus_tag="ACHKFK_002240"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO9052576.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWTGFNDPQEISGTLSADTTPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18411..19961)
                     /gene="cueO"
                     /locus_tag="ACHKFK_002241"
     CDS             complement(18411..19961)
                     /gene="cueO"
                     /locus_tag="ACHKFK_002241"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO9052577.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGGKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHSGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPATHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20127..20474
                     /gene="yacC"
                     /locus_tag="ACHKFK_002242"
     CDS             20127..20474
                     /gene="yacC"
                     /locus_tag="ACHKFK_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO9052578.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20580..21446
                     /gene="speE"
                     /locus_tag="ACHKFK_002243"
     CDS             20580..21446
                     /gene="speE"
                     /locus_tag="ACHKFK_002243"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO9052579.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTNQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNHGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            21462..22256
                     /gene="speD"
                     /locus_tag="ACHKFK_002244"
     CDS             21462..22256
                     /gene="speD"
                     /locus_tag="ACHKFK_002244"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO9052580.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTTEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSEDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITTALWKEMREIYYGRNMPAV"
     gene            complement(22294..22656)
                     /gene="yacL"
                     /locus_tag="ACHKFK_002245"
     CDS             complement(22294..22656)
                     /gene="yacL"
                     /locus_tag="ACHKFK_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO9052581.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22831..25428)
                     /gene="acnB"
                     /locus_tag="ACHKFK_002246"
     CDS             complement(22831..25428)
                     /gene="acnB"
                     /locus_tag="ACHKFK_002246"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO9052582.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAVAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRILLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFDQLSQYTEKA
                     DGVIFQTAV"
     gene            25783..27540
                     /gene="yacH"
                     /locus_tag="ACHKFK_002247"
     CDS             25783..27540
                     /gene="yacH"
                     /locus_tag="ACHKFK_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO9052583.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVEAQSTQ
                     QTAPDISAVTLPATVSVPPVTPAVVKSTFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSGQSWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKIITTTKKVVPVNQPANAPATQSN
                     TVSTSSPVVAEPAPTVIAIEPANPDVVYIPNYNPNVVYGSWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNDWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRDGMPYHDQDMAKRFHQTDVKGGMSA
                     TPLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHNGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAAREKMQTNPQNQQRREAARQRIQSASPEQRQ
                     AVREKMQTNPQNQQQRDAARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTARLNNE
                     QRSAVRERLSERGARRLER"
     gene            complement(27782..29206)
                     /gene="lpdA"
                     /locus_tag="ACHKFK_002248"
     CDS             complement(27782..29206)
                     /gene="lpdA"
                     /locus_tag="ACHKFK_002248"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO9052584.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29414..31306)
                     /gene="aceF"
                     /locus_tag="ACHKFK_002249"
     CDS             complement(29414..31306)
                     /gene="aceF"
                     /locus_tag="ACHKFK_002249"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO9052585.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMIFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31321..33984)
                     /gene="aceE"
                     /locus_tag="ACHKFK_002250"
     CDS             complement(31321..33984)
                     /gene="aceE"
                     /locus_tag="ACHKFK_002250"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO9052586.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34145..34909)
                     /gene="pdhR"
                     /locus_tag="ACHKFK_002251"
     CDS             complement(34145..34909)
                     /gene="pdhR"
                     /locus_tag="ACHKFK_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO9052587.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8899 a         9116 c         8413 g         8671 t
ORIGIN      
        1 gctgtcccaa tgatgaatga atgtaatact tagtaaaaaa caaacaaaca tcattaacaa
       61 agtaacgttc agttacatgg cattgcatca aaatctcatc atcatgcaat gctattatct
      121 gctttatgga atattactca aggattttta tgtctaaaaa attaggtttt gcgctgtccg
      181 gtattatgtt ggcaatggct gctggtactg catttgctgc taacgatatg gacggcggtc
      241 aattaaatat tagcggtctg gtcgttgata acacttgtga agctcgtgtt gatggcggta
      301 acaaagacgg tctgatcctg ctgcaaaccg ctactgttgc tgaaattact gatggtgtac
      361 tggataccac tgtaggtgct aaagctaaac cattcagcat tactattgac tgcagcaaag
      421 ctaacccggc tccgggtact actgcaaaaa tgaccttcgg ttctgttttc tttggtaaca
      481 gcaaaggaac actgaacaac gacatgtcta ttaacacccc gtctgacggc gtaaataccg
      541 ctctgcacaa tattgaaggt tctactatta aacaggttca ggtcaataat ccgggtgatg
      601 tttacagcaa aactctggat tcaacgtcta aatctgcaac ctacgatttt aaagcatcct
      661 atgttcgtgc ggatgcaagc aaagcagcta ctgccggtta tgtaaaaacc aactcagcat
      721 ataccattac ttaccagtaa atatcaaccg cttaaattga tcgggtgaat atatacaggg
      781 aataacatca aatattttta cttttgataa ttattccctg ttttaattaa ttctgtaagg
      841 aatggtttat gttttttaat actaaacata ccgcagcatt gtgcttagcg agctgtatgg
      901 cttttagctc atcagctatt gcagacatcg taatttcagg tacacgcgta gtttataaaa
      961 gcgaacaaaa aagcgtcaat gtacgtctgg aaaataaagg caataaccca ttgctggttc
     1021 agagttggtt agatactgga gatgataacg ctgaaccagg cagtattact gtccccttta
     1081 ccgctacgcc gccagtatca cgtattgatg ctaaacgtgg ccaaacaatc aaactgatgt
     1141 acaccgccag cagtgctctg ccaaaagaca gagaaagtgt gttctggttt aacgtactgg
     1201 aagttccacc aaaacccgat gcagcaaaag cagcaaacca aagcctgcta caactggcat
     1261 ttcgcacacg tataaaactg ttctatcgcc cggaaggatt gagtggaatt tcatctgatg
     1321 caccattggc tctgaagtgg tcctgggcaa catctgaagg caaagcagca ttacgcgttg
     1381 ataaccctac accctactat gtctctttta gcagtggtga tttagaagcc aacggcaaac
     1441 gctaccccct ggatatgaca atgattgcgc cgttcagtaa cgacgtcatc aaggtaaatg
     1501 ggatgagtgg cagaacaagc tctgcaaaag tgcatttttt cgccattaat gattttggtg
     1561 gatctattga aggcaacgcc aacctttaat ctggcaagga tataacacag caggaagcag
     1621 aacgtgacta ttaaatctac taatcacctt acccacatcg ccacgttttg cgctctgctg
     1681 tatagcaata gtgctttatg cgctgaactc gtcgaatatg accatacatt tctgatggga
     1741 aaggatgctt caaatatcga ccttagtcgg tatacagaag gcaacccgac actacctagc
     1801 atttacgatg tcagtgtgta tgttaacgat caacccatca tgagccaaag cattgctttt
     1861 gctgttattg aaggtaaaaa gaacgctcag gcctgcatta cgcagaaaaa tttattgcaa
     1921 tttcatatca gttcaccaga caagaatagc gaaaaagcca ttttgcttaa gcgagacgac
     1981 gatcttggtg attgcctcaa tctggctgaa atgatccctc aatcatccat tcgttacgat
     2041 gttaacgatc aacgcctgga tatcgatgtc ccccaggcat ggattatgaa gaattatcaa
     2101 aactatgttg atccctcttt gtgggaaaat ggcattaatg cagccatgct ttcctacaac
     2161 ctcaatggat atcacagtga aagtccagtc agaacaaatg acagtatcta tgcggcattc
     2221 aacggtggga taaacttagg cgcgtggcgg ttacgtgcct caggtaacta caactggatg
     2281 accaatgtcc atagcgatta cgattttcag aatcgctatt tacaacgcga tcttgcgtca
     2341 ctacgttcac aactggttat tggtgaatct tacacgaccg gcgagacctt cgattcagtc
     2401 agcattcgcg gtattcgctt atacagtgac agtcgaatgc tgcctccggt attagccagt
     2461 tttgcgccaa tcattcatgg cgtagcgaat accaatgcca aagtaaccgt tatgcaaaat
     2521 ggttataaga tctatgaaac gacggtgccg ccaggggctt ttgccattga cgatcttagc
     2581 ccgtcaggtt atggcagtga cctcattgtg actatcgaag aagctgatgg cacaaaacgg
     2641 acgttctccc agccattttc atctgttgtg caaatgctgc gccctggcgt tggtcgctgg
     2701 gatattagcg ccggtcaggt tttaaaagac agtatccagg atgagcctaa tttatttcaa
     2761 gccagctact actatggttt gaataactac ctgacaggat ataccggtat tcagctaact
     2821 gataataact ataccgccgg attgctgggg cttggtatga atacccccgt gggggctttt
     2881 tctgtcgatg ttactcactc aaatgtcagc attccggacg ataaaaccta tcaaggacaa
     2941 agctaccgta tttcctggaa caagttattt gaaaatacca gtacatcatt aaatatcgcg
     3001 gcctatcgct actccaccca gcactatctg ggcctcaatg atgcgctgac gctgattgat
     3061 gaagtagaac atcctgagca agatcttgag cctaaatcca tgcgcaatta ctcgcgcatg
     3121 aaaaatcagg tcacggtcag tatcaaccaa ccgctgaaat ttgagaaaaa ggattacggt
     3181 tcattttatc tctcgggaag ctggtcagat tactgggcct ccgggcaaaa cagtactaat
     3241 tactctattg gttatagcaa tagtgcgtcc tggggcagtt acagtatcag tgctcaacgt
     3301 tcgttgaatg aagatggtca aaccgacgat agcatttatc tcagcttcac aattccaata
     3361 gaaaatttac tgggtacgga acaccgcagc tccggcttcc agagtattga tacacaatta
     3421 aacagcgact ttaagggtaa taaccaactc aatatcagca gtagtggtta cagcgatacc
     3481 aatcgcatca gctacagcgt aaacacgggt tatatgatga ataaatccag tgatgatttg
     3541 agttatatcg gcggttatgc cagttatgaa tcgccgtggg gaacgctgtc aggttcagcc
     3601 tcagcaagca gtgataatag tcgccaattc tcactcaata ctgatggtgg atttgtgtta
     3661 catagcggcg ggctgacgtt cagtaatgac agttttagtg attccgacac tctggctgtc
     3721 attcaggcgc caggagcaaa aggcgctcgt attaactacg gaaacagcac cgtagaccgc
     3781 tggggttatg gtgtgaccag cgccctttcg ccataccatg aaaatcgtat cgcactggac
     3841 attaacgatc tggaaaacga tgttgaattg aaaagcacca gtactgtcgc cgtcccgcgc
     3901 cagggagccg tcgtctttgc tgattttgaa acagttcagg gacaatcggc aattatgaac
     3961 atcgtgcgaa gcgatggtaa aaatattcca tttgcagcgg atatttatga tgaacaaaac
     4021 aatatcatcg gcaacgtagg tcagggtggt caggcctttg ttcgcggtat cgggcaggaa
     4081 ggaaatatac gaattacctg gattgaagag ggcaaaccgg tcagttgttt tgctcattat
     4141 caacaaaaca cgacatcaga aaaaatagca caatctatta ttctgaacgg acttcgttgt
     4201 cagatacagt aaccacaagg gacttccaat gataaaaatc acaccacata aaataacaat
     4261 actcatggga ctattgttat cgccttcggt atttgcaacg gatgttaatg tcgattttac
     4321 cgctaccgtg aaagcaacaa cctgtaatat tacacttacc ggaactaatg tcactgataa
     4381 tggaaaagat aagtacacct tagttattcc ctcgatggga atggataaaa tagccaataa
     4441 aacagcacaa tctgaagcca actttaaatt ggtagccaat ggatgcagca gtggaattag
     4501 ctggattgat acaaccctga caggaaatca atcaggaagt tcacccgcgc taattatccc
     4561 cctggccagt gatacaacct ccactacaag ctatatcgga atgggattta aaaggaaggc
     4621 gacttcaggc gatacatttc tgaaacctaa tagcgcagaa tatatacgtt ggagtgcatc
     4681 agaaattagc accgatggac ttgagatgac tgtcgcactg cgtgaaacaa gtgtagggaa
     4741 aggtgttcca ggcaagtttc gcgcactggc aacttttaac ttcagctatc aataacaagg
     4801 atgacaatga tgatattaaa aaaattatgt tatggattat cgggtagtct atttctggct
     4861 tcatcactgt taaccatttc ctcacatgcg gccactgaca gtattggcct gactgttact
     4921 actgacgtag aaatgggtac ctgtacatca acgttgactg atgacggtac aactaaaatt
     4981 tccaccatca atttcggtga tgtatatatt tctgaaatta atgccgaaac taaaattaaa
     5041 accttcaaat tacagttcaa agactgtgcg gggattcccg ggaaaaaggc caaaataaaa
     5101 ttgactacac gagcattatg tgaaggtaat tctaataatg gtcctggatt tgctaacgca
     5161 tcaacggcaa cagcaaaagc agcggcggtc gccgttgagg tctggagcac atctacgcca
     5221 ggtaagaacg gcgctaaaca attcagttgt gtcacccccg caacagaaga agtcagtatt
     5281 gctaatgcaa cgggcagcaa tgttgtcgat tacccaatga gtgcggtgtt ggtggtcgca
     5341 aaagataaaa ctgtcacaaa tgtcactgcg ggtgatttca ctgccccggc aacattcacc
     5401 gtgacttata actaacaggc agactgattc aggaactaaa tatgcgtccc actcaacgta
     5461 atctgatgaa aaaatcaatt ctgattctgt cgttgctatt cagcgccact tcccctgtgg
     5521 ttgcaggaca ggatgttgac attgttgcaa atataaaaaa taacacctgc caaagtggaa
     5581 tcagcaacaa cggaaatatt gatttaggtg tcgttggcgt gggcgatttt accggaaaca
     5641 tttcagccga aaattatcac cccggtggaa aagagttcac tattacagta caggactgca
     5701 cacttcaggg tacagggaat gtgttaaatc agttacatat taattttcgc gcacttagtg
     5761 gcgtaatggc gacaggttcc agtcaaatat tcgctaatga agacaacgcc ggggctaaaa
     5821 atgttggggt agttatattt tctattcagg acccggcaaa taccttcaac gttcttagtg
     5881 ccactggtag ctcacgttcc gtttatccgg tcatgtcatc agcactaaat aattcatcct
     5941 ggaaatttag tacccgaatg caaaaaatcg atccagcact gaatgtcacc tctggcccac
     6001 tgataagcca tgtgttagta gatatctact acgaataaca gacaaagtgc tacaagtatt
     6061 atcttgcagc atgacaatgg agtaaaaatg aagacattct tcagatactt tcttttttta
     6121 gcactatgct cttgttgtta tacagcaagc gccggcaccg atgatgacgt cggctatatt
     6181 gttgggaata attacggtgt tgggcctgga gaccaaaaat ggcgcgaaac aggccccaat
     6241 ggagatgtta ccgtcaaatt ccggtatggt tcaggcacca ataatttagt tttttataaa
     6301 ccaacccaac ttggcccaac cggggtgtca ttaaaatggg cgcagttaga ttccgcttcg
     6361 ggtggaggtt tcctttactg taacaggagt gataactcaa gcggcgcacc aatgtctatt
     6421 gaacataaaa tggttgattc cggaaaaagt tatggcggac ataaactctt taagacatct
     6481 gttccaggcc tgtattacac gttagcaata tcaaacattt ggtcaacata tacatatact
     6541 gatatcaacc catcaggcat gtatattggc gattctacga gccaatcttt taactggcgt
     6601 ggcgaaagcg agcaaacact atattggtct tgcaacaacg cgaatagcag caaaaaatac
     6661 tgggccgttg gtggcgttat gcaaacgctc acgattgaat tttataccga taccgacttt
     6721 aacccgacaa cgaatcaacg ggtaacatta tccagaacag atagttatct ctactcattc
     6781 aaagcatata atgctggcat tagtatcaag agttattttc ttaaaatcga ctttgacctg
     6841 acagatatcg tattaacaaa ccctacctgt tttactgctg cgcttagtgg cccatcggta
     6901 aatggttcaa cggttaaaat gggtgattat agccctgcac aaataaaaaa tagcgcaaca
     6961 gcggttcctt ttgatatcac gctacagaac tgtattcgtg ttcgcaatat agaaacaaaa
     7021 ctcaagtcaa acaaggtggg tagtgtaagc aaagaacttc tggctaacac actcacagga
     7081 aatgacgcag caaaaggcgt tggtgtactg attgaaggtt taaaaaacac taaaagtgca
     7141 caaatggtac tgaaaccaaa taatgcaacg tctatttata aagattacga aacagaagat
     7201 gacactaccg gaggaattta cccggataaa ggaaacggta cctcacaacc tcttcatttc
     7261 caggctacat taaaacaaga cggtaatatt gctatagaac cgggggattt taaagccacc
     7321 agcacttttc aggttaccta cccctgataa cactatcgca ccgccagaca acggcggtgc
     7381 gatacggata aatcattgcc cccaccaatg tgactgataa aatgcgagac agcacaacat
     7441 catttatcag gatacgctat gaaaccaacc acgattgctt cattgcagaa atgcaagcag
     7501 gacaaaaaac atttcgcaac catcaccgct tacgactata gcttcgcaaa actgtttgcc
     7561 gatgaaggga ttaacgtcat gctggtaggc gattcactgg gcatgacggt ccaggggcat
     7621 gactccacgc taccagttac cgttgccgat atcgcctatc acaccgccgc tgtgcgtcgc
     7681 ggcgcgccaa actgccttct gttagcagac ctgccattta tggcgtatgc cacgccggaa
     7741 caagcctttg aaaacgccgc aacggtaatg cgtgccggag ccaacatggt caaaattgaa
     7801 ggcggtgagt ggctggtgga aaccgtacaa atgctgaccg aacgtgccgt tccagtttgt
     7861 ggtcatttag gcctaacccc acagtcagtg aatattttcg gtggctacaa agtccagggg
     7921 cgcggcgatg aagcgggcga tcgactgctc agcgatgcat tagccttaga agctgctggg
     7981 gcacaactgc tggtactgga gtgcgtgccg gttgagctgg caaaacgcat taccgaagca
     8041 ctggcaatcc cggttatcgg tattggcgca ggcaacgtca ctgacgggca aattctcgtg
     8101 atgcacgacg cgttcggcat taccggcggt catattccta aattcgccaa aaatttcctc
     8161 gccgaaacgg gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc
     8221 gtttacccgg gcgaagaaca cagtttccat taaggagtga cgttgtgtta attatcgaaa
     8281 ccctgccgct gctgcgtcag caaattcgcc gcctgcgtat ggaaggtaag cgcgtggcgc
     8341 tggtgcccac catgggtaac ctgcacgatg gccatatgaa gctggtcgac gaagccaaag
     8401 cccgcgccga tgtggtcgtc gtcagtattt tcgttaaccc aatgcagttt gaccgcccgg
     8461 aagatctggc acgttatcca cggaccttgc aagaggactg cgaaaagctg aacaaacgta
     8521 aagtggattt agttttcgcg ccttcggtaa aagagatcta cccgaacggt actgaaaccc
     8581 acacctatgt tgatgttccc ggtctttcca ccatgctgga aggtgccagc cgtccgggac
     8641 attttcgcgg cgtttcgact attgtcagca agctgttcaa cctggtccag ccagatatcg
     8701 cctgcttcgg tgaaaaggat tttcagcaac tggcactgat ccgcaaaatg gttgccgata
     8761 tgggcttcga tattgagatt gtcggagtgc cgattatgcg cgcgaaagac ggtctggcgc
     8821 tcagttcccg taacggctat ctgacggcgg aacaacgtaa aattgcgccg ggtctctaca
     8881 aagtcttaag ttcaattgcc gacaaattgc aggctggcga acgggatctc gatgaaatta
     8941 ttgctattgc cgggcaagaa ctgaatgaaa aaggtttccg ttccgatgat attcagattc
     9001 gcgatgccga caccttgctg gaaatctctg aaaacagcaa acgagcggtg attctggtag
     9061 ccgcctggct tggcgatgct cgcctgatcg acaacaaact ggtcgaactg gcgtaatgct
     9121 taactggcgc tacggctgat ggcgccagtt attcattgcc acgttatctg taggcaatta
     9181 ttcataatgc agacaactta gttaatcatt ttatcaatct cagcaagagg taaatttacc
     9241 atctctgcaa cgtcttcccg agacattcct ttactcagaa gacgcagggc gaattcctga
     9301 cttacttcct gtcttccctg ctgaatcccc ttctcttcaa accactgcgc cagcgtcatc
     9361 atagactccc ctcccgtttc cctgtctctc accacaccat aaaacaaatc cgcttgttca
     9421 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     9481 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     9541 tgtttttgca gcagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     9601 gtgatagtga tatccaccag cgggaaagga ctgttataga cgcgtcgcgc cagttccggt
     9661 gagtaaaaca tatcaaacca gcacattgat agcggataag gtgtggcctc gccctgataa
     9721 aacagaattg gcaccaccag cggcagctta tcgtggtcag cctccagatg ccggtgcatg
     9781 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttgatgt
     9841 tcaatcacaa catgcaaata accggggttg ccctgcattt gcacggaata aagcacgtca
     9901 gtgctgtgcc ctttcaggct ttcttcaata aaactccccg actcaagatg cagcgtgtcg
     9961 aggtcacaca gttcgcgtag ctccgctggc aaatggatat ccagaaagtc gcgagccgtt
    10021 tccgcatgca ttaaaaattg tttaaacacc gcatcatgcg gtgtggtact cggtgcatcc
    10081 atcttccctg ccttccgtgt ggtcttgcgt gaaggcagta tccagtcaag gtgtgccgtc
    10141 gtcagccgcc tctttagtgt ttaccgagca gtgttcagat aaatttcaat tggttacagg
    10201 cgttacatgg cacgcttcgg cacggtacgc aaactgctgc tgcaaatgcc gtcagatgag
    10261 tagacactaa acaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc
    10321 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
    10381 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
    10441 atcaggattt tcttgacgca gccggtattc tcgaaaacga agccattgat atctggaatg
    10501 tcaccaacgg caagcgtttc tccacctatg ccatcgcggc agaacgcggt tcgagaatta
    10561 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
    10621 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaacgtc gcctattttg
    10681 aaggcgacaa cgaaatgaaa cgtaccgcga aagcgattcc ggttcaggtt gcctgattgt
    10741 tatccctgcg gctggttact caccagccgc gacatcgtct ccagcgaatc cgttcttaag
    10801 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtc
    10861 gtcaccgcga ggtgaaatcc ggcttctttt gctgccttca cggctttgtc attaaatccg
    10921 ccaaatggat aagaaagata caaaacatgt ggattaaatt gcgccaaagc gcggcgtgaa
    10981 cgtgcaaaat caaacagaat attgtgctca ctacggctca gcaatatagg ccgccgataa
    11041 ccatctatcc gatgcaaaaa atgagtatgt gactggaaat caaatacatc gcgaatttcg
    11101 ttaagttcag aaacgctcat aaattgcagc gattttgggt tccatttctg cgggtgacgt
    11161 ttgatgcgcg aggtaacaat aaacgccgtc gccttcatac catattgttt caacacagga
    11221 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taattaccac cgctcgcgca
    11281 gggagattga tcttattctt cacgtaacct tccaactgcg ccatgctcag tgtcgcgtat
    11341 cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
    11401 gtatggcgaa aacgggtgtt ttcttcgtcg cgcaaaatat ggtgataggt tagcaccggc
    11461 aggccattat cgggttgggc atccagcgcg ctgatatagg ccaatcgatc gccaatacgg
    11521 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
    11581 ttgtccgcca gtactccaaa tggcgcactg cccacactcg gcgcgttata aaccggcgta
    11641 tctttccagg ttattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccatct
    11701 tcaacttttt gtcgcccctg aaccggctcg agatggcctt tatcgataaa ccctttacca
    11761 aagccaaaat taaatacgta ataacttgcg gcagtgggct ccacagcaat gatttgcccg
    11821 gcccgaatat tccctaccgt caccattttg tcgccaattt gcgcccagac cgcggcattt
    11881 tcgatggttt gcatataacg ggcaggtaat gtggcactga cgcttgcggt aaacagcatc
    11941 agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa acaacgcttt
    12001 tattgtagca aaagcgtcat cagaatcgcg ggttaatact catacaaatt atgtttgagg
    12061 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cgcacccagt
    12121 tcgacaatac gctcgcgaaa cgctgaactg gtcatggttt cacggcaagc catcatctgt
    12181 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gagagtgttt gtggcttaat
    12241 aatgaagcca cgcggtacgg tggcgctccg gctatatcgg ttaaaaagat aacgccctca
    12301 ccagagtccg tttcatgcaa ggcatcgcac atcatgcggc tgagcatatt agagcttaat
    12361 ccgcgccaga aattcacggc ccggcactga agaagtgccc catgtttttt ctccagcgca
    12421 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
    12481 ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat agccgtcaaa
    12541 actcctctcc cccaaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
    12601 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
    12661 aggtagtgac cagcggaaca tcattgatac cgaggaagcc gtagcggaaa ccactgatca
    12721 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcaggg
    12781 agtaaaacac cccgcccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
    12841 tgtcatcgaa cgttttagca aacacgccgt tcagcaaacc cgcaagggag aatagcaccg
    12901 cagtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
    12961 acagtgaaat tgccgtcacc agaataccaa caaacagacc acgcgccacg ccaccaccga
    13021 catatccggc aataatgaca tgagtcggaa ccggcgctac cagcagctct tcaatattac
    13081 gctggaactt ggcaccaaaa aatgatgacg cgacgttagc gtaggcgttg gtgatcaccg
    13141 acatcatgat cagccccggt acgatgaact gcatgtagct gaagccatgc atatcgccaa
    13201 tacgcgaacc aatcaggtta ccgaagataa taaagtaaag ggtcatcgtg atgactggcg
    13261 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
    13321 ttagcgccac ccagtaaaga tgcatcatgt gcgatctcct tgtttttcat taaccagtga
    13381 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
    13441 ctcacttaac tgcgtaaata cgctgttgat gccctgctca cgcagcactt caacttccag
    13501 cgtggcggta tctaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
    13561 tgcgagatcg agaaaaaagg tttctgattt cagcttcgcc agcagcgcct tcatcgaagt
    13621 attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca tttctgcttc
    13681 ttccaggtaa tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
    13741 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
    13801 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
    13861 taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agttgtttaa gatacttttc
    13921 gctgcgaatg tacgcttctt tgcgctccac accgtagtac cctgcctgat tcaccacaat
    13981 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgt ggcaccagac ccaactgacg
    14041 tttagcgttt acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
    14101 tttatttacc agagagctga taataccgat agtggtcgat tttccggccc cgttcggccc
    14161 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatcg attccacgaa gcgcctgaac
    14221 gccgcctgga taggtttttt taagctgttg aagttccagt gcaatggtca taaattttta
    14281 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
    14341 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
    14401 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
    14461 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
    14521 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
    14581 aacctggtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
    14641 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgca
    14701 gttgaaaacc cagaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
    14761 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
    14821 gaactgaacg tcatggaaca ggtgtataac ctgggccact ccactattat gcaatcagcg
    14881 tggaaacgcg ggcagaaagt caccatacac ggctgggcct acggcattca cgacggcttg
    14941 ctgcgtgatc tggatgtcac agggactaac cgcgaaaccc ttgagcaacg ttaccgtcac
    15001 gggatctcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
    15061 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg atatacggca
    15121 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
    15181 agaaaccgat aaattctacc gggacgttca cttcacgacg ggacggttta tccagcagcg
    15241 tacaaatcgc caacgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
    15301 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
    15361 ccaggtcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
    15421 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
    15481 taaatgagcc acgcagcaga cccaccagca ccatatcgct gccgctgtct ttgtaacgct
    15541 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
    15601 cttctacagt atgtttcata tctataacca tatgatttaa aaacaaatca cttcgcacgg
    15661 gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt
    15721 cgcggaatct gttaatacag ctcgaattgt gatgacgatc acacatgtta aaacctataa
    15781 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
    15841 ccggctcgcg gcgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
    15901 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
    15961 ctggccttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgc gccgcgcttt
    16021 ggctgtacgc agccctgctg ctcggcacta tgatttgggg cgtctgggaa gttggtttcg
    16081 acttctgggc gctgactccg cgcagcgaca ttctggtctt cttcggcatc tggctgatcc
    16141 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
    16201 tcgcactgct gattagcggt ggtatcctga cctggaccgg atttaacgat ccgcaggaaa
    16261 tcagcggcac cttaagcgcc gataccacgc cagcagaagc catctccccc gttgccgatc
    16321 aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacca ctgaagcaaa
    16381 ttaacgctga taacgtccac aagctgaaag aagcctgggt gttccgtact ggcgatgtga
    16441 agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccaattaaa gtgggcgaca
    16501 ccctttacct gtgtaccgcg catcaacgtc tgtttgcgct ggatgccgcc agcggtaaag
    16561 agaaatggca ttacgatcct gagctgaaaa ccaacgagtc cttccagcat gtaacctgcc
    16621 gtggcgtctc ttatcatgaa gccaaagcag aaactgcttc gccggaagtg atggcggatt
    16681 gcccgcgtcg tatcattctt ccggtcaatg atggtcgtct gattgcaatt aacgctgaaa
    16741 acggcaagct gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
    16801 cggacaccaa accgggtctg tatgagccga cttcgccgcc gattatcacc gataaaacca
    16861 tcgtgatggc cggttcagtc accgacaact tctcaacccg cgaaacgtct ggcgtgatcc
    16921 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatccgggc gcgaaagatc
    16981 cgaatgcgat cccgtctgac gaacacacct ttacctttaa ctcaccaaac tcgtgggcac
    17041 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccag
    17101 atatctgggg cggtaatcgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    17161 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    17221 atatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    17281 cggtcattta tgctccggcg aaaaccggca acatttttgt gctcgatcgt cgcaatggtg
    17341 aactggtggt tcctgccccg gaaaaaccgg ttccccaagg tgctgctaaa ggcgattacg
    17401 tgacccctac tcaacccttc tcggaactga gcttccgtcc gacgaaagat ttgagcggtg
    17461 cggatatgtg gggagccacc atgtttgacc agttggtgtg ccgcgtgatg ttccaccaga
    17521 tgcgctatga aggcattttc acgccgccat ctgaacaggg tacgctggtc ttcccgggta
    17581 acctggggat gttcgaatgg ggcgggattt ccgttgatcc gaatcgtgaa gtggcgattg
    17641 ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtcct ggtaacccga
    17701 tggaacagcc gaaagatgcc aaaggcacgg gtacggaatc cggcatccag ccgcagtacg
    17761 gtgtaccgta tggcgtcacg ctcaacccgt tcctctcacc gtttggtctg ccatgtaaac
    17821 agccagcatg gggctatatc tcagcattgg atctgaaaac caatgaagtg gtgtggaaga
    17881 aacgtattgg tacgccgcag gacagtatgc cattcccaat gcctgtaccg gtgccgttca
    17941 atatgggtat gccgatgctt ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    18001 ccgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    18061 agggtcgttt accagcgggt ggtcaggcta cgccaatgac ctatgaagtg aatggcaagc
    18121 agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    18181 ttgtggctta tgcgctgccg gatgatgtga agtaaggctt gctcagattg ctgacaacgt
    18241 gcgatttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa acatttgcaa
    18301 attcaataca ttgcgcgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgtc
    18361 atcagtttca tgcccggaga gatccgggca tatttccgaa tacggtcttt ttataccgta
    18421 aaccctaaca tcatccccgt atcttcatgc tccagcagat ggcagtgcgc catataagca
    18481 tgttctttcg gtgcatcgtg attaaacttc accagcactt cgctgacatt accttctacc
    18541 ttaacggtat ctttccagcc tgcgcgatgc gtcgctggcg gtttgccatt ttctgacaag
    18601 atacggaact gcgtaccgtg gatatggaac ggatgcagca tcatgtcgcc cacgccagag
    18661 ataacccaac gttcgtactg ccctttcgcc gccgcaaaca tcggcttatt catatcaaac
    18721 gcctgaccgt tgattttgtt ggcatggtgg aaatcgaact tcccgctgtg gttcatatga
    18781 ttcatattgc cgtgccccat atggcccatc atctggctgt gatccatccc ggccatcgcc
    18841 tgatcgccat atttctccat cagcatctgc atccccatca tatcgagcat tgggtccata
    18901 gaaagttgca gcttgcgtac cgtcagccct tccagcgaag gtaacgcagg caggctactt
    18961 aatgtgtctg gcaaagcacc agaggcacta atggcaatcg gctgaatccg cattaccgga
    19021 tgcggcttgt caaacggcgc aatcgccatc cccatctggc tgaccggcag cgtcaccagg
    19081 tcaaagggtt tgttgtcgtt aacctccacc agcacttcaa aacgctcgcc catcagcact
    19141 ggcagctcgt tcaccttcac cggttcaggt agcagaccgc cgtcgctggc aatcacataa
    19201 agcgggcgat tgtcgctggt ggcgaaattg agcgagcggg cattacagcc attgagcaaa
    19261 cgcaggcgca gccagccacg cggggcagcg tgttgcgggt agattgcacc gttggtcagc
    19321 aacgtatcgc caaaccaacc cacagcggcg gtcatcacat ccagttgata atcaatctgc
    19381 ccgtcagcgt taaatttttt atcctgaaca atcaccggga catcatcaat accccactgt
    19441 tttggcagca ttaatttcag gatctcgtca tcttcaatca ccaccaaacc agccagcccc
    19501 atcgccacct gacgtccggt cttgccatgt tgatgtggat ggaaccagca ggtagcggca
    19561 ggttgatcaa cgttcaacgt caccgagcgc ttgccacctg gcggaataat tccctgcggg
    19621 ccgccgtcca cttcacccgg tacttccagc ccgtgccagt gcaacgtcgt ctcttccgtc
    19681 agttggttgt agatatcaac cgttaccgct ttgccacgct gtaatttcac cgccggcccc
    19741 agcagattgc cgttatagcc ccaggtagtt gcggttttcc cgccaaaggt ggactgacct
    19801 gcgccaatag ttaactgaat gcgattacgg gcatcggtcg tgagcaaatc agggattggt
    19861 aacgttgggc gttccgccgc aaatactgcg cggctccaca gcggcaaggc tgaagccaca
    19921 cccagcgcga cggaatattt caagaaatca cgacgttgca tagttatttc cttattctta
    19981 agcaggcgaa acaaaatcaa acgttgagca tagtccttcc ccttacggga aggtcaagcc
    20041 gcagacacaa tttaatcgac aataatggtc gtcgcctcgc tcgcagtgtg ctaacgttta
    20101 tcttctttaa gcccctggta gaagcaatga agacgttttt cagaacagtg ttattcggca
    20161 gcctgatggc cgtttgcgca aacagttacg cgctcagcga gtctgaagcc gaagatatgg
    20221 ccgatttaac ggcagttttt gtttttctga agaacgattg tggttaccag aacttaccta
    20281 acgggcaaat tcgtcgcgcg ctggtctttt tcgcccagca aaaccagtgg gatctcagta
    20341 attacgacac cttcgacatg aaagccctcg gtgaagacag ctaccgcgat ctcagtggca
    20401 ttggcattcc cgtcgctaaa aagtgcaaag ccctggcccg cgattcctta agcctgcttg
    20461 cctacgtcaa ataatccctg ataccttttt gtagaaatat tgaccgtgca tccgcggtca
    20521 atgttagcta ttatgttgcg cccttttttt acgggtgtta acaaaggagg tatcaaccca
    20581 tggccgaaaa aaaacagtgg catgaaacgc tacacgacca gtttgggcag tactttgcgg
    20641 tagataacgt tctgtatcat gaaaagaccg atcaccagga tctgatcatt tttgagaacg
    20701 ccgcatttgg tcgcgtaatg gcgctggatg gcgtagtaca aactaccgag cgcgacgagt
    20761 ttatctatca tgagatgatg acccatgttc cgctactggc ccacggtcac gcgaaacatg
    20821 tgctgattat cggcggcggc gacggtgcca tgctgcgtga agtaacccga cataaaaacg
    20881 ttgagtcaat cacgatggtg gaaatcgatg cgggtgtcgt gtcgttctgc cgtcagtatc
    20941 ttcccaacca taacgccggt agttacgacg atccgcgctt taaactggtg atcgacgatg
    21001 gtgtcaattt cgttaatcaa accaaccaga cctttgatgt cattatctcc gactgcaccg
    21061 atcctatcgg tcccggcgaa agtctgttta cttctgcatt ttatgaaggc tgcaaacgtt
    21121 gcctgaatca tggcggtatc ttcgtcgcac aaaacggcgt ctgcttttta cagcaggaag
    21181 aggccatcga cagtcatcgc aaactcagcc attacttcag cgatgttggc ttttatcagg
    21241 cggcgatccc gacctattac ggcggcatca tgacctttgc atgggcgaca gataacgacg
    21301 ccttacgcca tctctcaacc gaaattattc aggcgcgttt tctcgcttct ggcctgaaat
    21361 gccgttatta caatccggca gtccatacgg cagcttttgc cttaccccag tatctgcaag
    21421 atgcactggc ttcacagccg tcctaaggag aagataagaa attgaaaaaa ctgaaactgc
    21481 atggctttaa taatctgacc aaaagtctga gtttttgtat ttacgatatc tgctacgcca
    21541 aaactaccga agagcgcgac ggttatattg cttatatcga tgaactctat aatgccaacc
    21601 gtctgaccga aatcctgtca gaaacctgtt ccattatcgg ggccaatatt cttaacatcg
    21661 cccgccagga ttacgagcca caaggtgcca gcgtcactat tctggtgagt gaagaaccgg
    21721 ttgacccgaa actcatcgac aaaacagaac accccggccc actgccagaa acggtcgttg
    21781 cccatctcga taaaagtcat atttgcgtac atacctaccc ggaaagtcat cctgaaggcg
    21841 gtttatgtac cttccgcgcc gatattgaag tctctacctg cggcgtgatt tctccgctga
    21901 aggcgctgaa ttacctgatc caccagcttg agtccgatat tgtgactatt gattatcgcg
    21961 tgcgcggttt tacccgcgac attaacggta tgaagcactt tatcgaccat gagattaatt
    22021 cgattcagaa ctttatgtcc gaagatatga aggcgctgta tgacatggtg gatgtaaacg
    22081 tctatcagga gaatatcttc cataccaaga tgttgcttaa agagttcgac cttaagcact
    22141 acatgttcca caccaaaccg gaagatttaa ccgacagcga gcgccaggaa attaccactg
    22201 cgctgtggaa agaaatgcgc gagatttatt acgggcgcaa tatgcccgct gtttaacgtc
    22261 tcaggcggag cacggaggct ccgcctgatt tacttacttc tgctgcacga aattacggta
    22321 agccgccacc acctgaagaa aatcctcaac gccgcacagc gacaagctct cttcatcgta
    22381 gtagttcatc ccctcttcca tttcatcgcc agcgaattcc agttgattgg cgcgaaccat
    22441 cacctcttca ccatccatcc atagcgtgta ttcatgccct gcccgttgcc aggaacgttc
    22501 gctacctttc aatgcgtgcg ccgcttgttc tacttcatca agcaaggcca ggttttcttt
    22561 cacctcttca ttaaaccagt gcccgaccac ttcatgcccc atggacatgc gcacctttac
    22621 cactccggta atatcgcgca gaaattcgta atccatagtg tgttcctctg ctcctggcaa
    22681 tgcgccgaaa tcgcattgcg ccattgatgt aattatcgca gccttaccgg agaaaaacag
    22741 cggaacggcg atggtttaga aataaaaaat gcccgggaat ctcattcacc gggcattgtg
    22801 tcgtttatgc gcagcgcgtg cgcttactta ttaaaccgca gtctggaaaa tcaccccgtc
    22861 cgctttttcg gtgtactgag aaagctggtc gaagttcaga taacggtaag tatcaacggc
    22921 tgttttatct acctgcgcca cgtaggtctg gtactcttcc ggcgtcggca gtttgccaat
    22981 cagcgccgca acagcagcca gttccgcaga agccaggaag acattcgcgc cagtacccaa
    23041 acggttcggg aagttacggg tagaggtgga aaccaccgtc gcaccgtctg ctacacgcgc
    23101 ctggttaccc atacacaggg aacagccagg gatctcgata cgagcaccac tcttaccgaa
    23161 gacgctgtag tagccttctt cggtcagctg cgcagcgtcc atacgcgttg gcggtgccac
    23221 ccacaggcgg gtcggcaact gacctttgtg cgcatccagc agtttaccag cagcacggaa
    23281 gtgaccgatg ttagtcatgc aagaaccgat aaatacttcg tcgatcttct cgccctgtac
    23341 cgcagacagc ggacgtgcgt cgtccgggtc attcggtgca cagaggattg gctctttgat
    23401 atccgccaga tcgatgtcga tcactgccgc gtattccgca tctgcatcgg cttccagcag
    23461 ctcaggattc gccagccatt tttccatgcc ctgaatacga cgctccaggg tacgacgatc
    23521 gccgtaacct tccgcgatca tccacttcag caggacgatg ttagagttca ggtattcaat
    23581 gatcggttct ttgttcagct tgatggtaca accggcagca gaacgctcgg cagacgcatc
    23641 ggtcagctca aatgcctgct caactttcag atccggcaga ccttcaattt ccagaatgcg
    23701 gccagagaag atgtttttct tacctttctt ctcaacggtc agcagacctt gtttgattgc
    23761 atacagcgga atggcatgta ccagatcgcg cagggtgata cccggctgca ttttgccttt
    23821 gaagcgtacc agaacggatt ctggcatatc cagcggcatt acgcctgttg cggctgcaaa
    23881 tgccaccaga ccagaacccg ccgggaaaga gataccgatc gggaaacggg tatgggagtc
    23941 accaccggta ccgacggtat ccggcagcag catacggttc agccaggagt ggattacgcc
    24001 gtcacccgga cgcagcgaca caccgccacg gttcataatg aagtccggca gcgtgtggtg
    24061 cgtgttcaca tcaactggct tcggatacgc cgcggtgtgg cagaaagact gcatcaccag
    24121 gtcagccgag aagcccaggc acgccaggtc tttcagttca tcacgggtca tcgggccggt
    24181 ggtgtcctgg gaacctacag aagtcatttt cggttcacag tacgcgcccg gacgaatgcc
    24241 tttcacgcca caggctcggc ccaccatttt ctgcgccagc gagaagccac ggtcgctctc
    24301 agcgacatct ttcgcctgac ggaacacatc gctgtgcggc aggccaagtg cttcacgcgc
    24361 tttggtggtc aggccacgcc cgataatcag cagaatacgg ccgccagcac gcacttcatc
    24421 aatcagcacg tcggttttca gttcgaaggt tgccagcagt tcgccggttt cgtggttacg
    24481 cacttcacct ttgtacgggt aaacgtcaat tacgtcgccc atgttcaggt tagagacgtc
    24541 gacttcgatt ggcagtgcac ccgcatcttc catcgtgtta aaaaagattg gtgcaatttt
    24601 accgccgagg cacaaaccac cgccgcgttt gttcggcaca tgtggaatat catcgcccat
    24661 aaaccacaga acggagttcg tggcggattt acgcgatgaa cccgtaccca caacgtcacc
    24721 gacgtacgcc agcgggaaac ctttctgttg cagagcttca atctgtttga tcgggccaac
    24781 aacaccaggc tgatctggct caataccttc acgggcgttt ttcagcatcg ccagcgcgtg
    24841 cagtgggata tccgggcgtg accacgcatc tggtgccgga gagaggtcat cggtgttggt
    24901 ttcgccagtg actttgaaga cggtaacggt cagtttttca gccagcgccg ggcgattcag
    24961 gaaccattcg gcatccgccc aggactgcat tacctgcttc gcatattcgt tgcctgcttt
    25021 ggctttctct tctacgtcat agaagttatc gaacatcagc agcgtgtgag aaagtgcttt
    25081 ggcagcgatc ggtgccagtt tggcatcatc cagcgcgtcg atcagcggat gaatgttgta
    25141 accaccctgc atagtgccca gcagttcaat ggctttttcc ggagtcagca gcggggattt
    25201 agcttcgcct ttggcgacag cagccaggaa gcctgctttg acataggcgg cttcatcgac
    25261 gccaggggga acacggttgg ttaacagatc taacaggaat tcttcttcgc ccgcgggcgg
    25321 gtttttcagc agctctacaa gtgcggccat ttggtttgca tccaggggtt tgggcgcaat
    25381 cccctcagcg gcacgctcag ctacgtgctt acggtattct tctagcacga cggttctcct
    25441 cgctctcatt gtcatagtgc ggcaggcgat tctcttcacg ctcctgtgag acagcagttt
    25501 gtagggtaaa tgcccagtac cgcaacgggc agaatagcag gattttgacg atgtgttaat
    25561 ctgtttacaa aaaagcaaca taaaaaagtg gctgaatcgt taaggatggt ctagagattg
    25621 tttccctccg taaaaacttc gttaattcgc atggtgataa tcacctttct caacgaatcc
    25681 caagattagt caaaatttaa actacggcct ctttatactc ggattcacag cacctgcggg
    25741 tggccgttcg ccgaccattg cgatttcctt gagatccgaa ttatgaaaat gactttgccg
    25801 tttaaacccc atgtgctggc actaatttgc agtgccgggc tttgtgccgc ctctgccggg
    25861 ctatatataa aaagccgcac agtggaagcg cctgtggaag cgcaatcgac acaacagact
    25921 gcgcctgaca tctccgcagt tacgcttcct gcaacggttt ccgtgcctcc tgtaacacct
    25981 gccgtcgtca aatctacatt cagcactgca caaatcgatc aatgggtcgc gcccgtcgcg
    26041 ctgtaccccg actctctgct ttcacaagtg ttaatggcat caacctatcc ggcaaacgtt
    26101 gctcaagcag tgcaatggtc acacgataat ccacttaaac aaggcgatgc tgctattcag
    26161 gcggtatctg gccagtcctg ggacgccagc gtcaaatcac tggtggcctt tccacagttg
    26221 atggcgttga tgggcgaaaa cccgcaatgg gtgcaaaacc tgggcgatgc ttttctggcg
    26281 cagccgcagg acgtgatgga ctcggtacag cgattgcggc aactggcgca acaaaccggc
    26341 tcgctgaagt catcaactga acaaaagatc atcacgacaa cgaaaaaagt cgtaccggta
    26401 aatcagcccg ccaacgcccc tgccacacaa tcaaataccg tttctacgtc cagccccgtc
    26461 gtcgcagaac ctgcaccgac cgtaatagcc attgagccag ccaatccgga tgtggtttat
    26521 attccgaact acaacccaaa cgtggtttac ggcagttggg ccaataccgc ttatccgcca
    26581 gtttatctgc cgccgccagc cggagaaccg tttgttgaca gctttgtgcg cggattcggc
    26641 tatagcatgg gcgttgctac cacatacgca ctattcagca gcatcgactg ggacgacgac
    26701 gatcatgacc atcatcatca tgacgatgat gattatcatc accacgatgg cggtcatcgt
    26761 gacggtaatg actggcaaca caacggcgac aacatcaata tcgacgtcaa caatttcaac
    26821 cgtatcaccg gtgagcatct tactgataag aatatggcat ggcggcacaa tccgaattac
    26881 cgcgatggta tgccttatca tgatcaggat atggcaaagc ggtttcacca aaccgatgtt
    26941 aaaggcggaa tgagcgccac gccgctacct gctccaacac gcgacagcca gcgtcaggcg
    27001 gcagcaagcc agtttcagca acgaacacac gccgcccccg tcattacgcg agatacccaa
    27061 cgtcaggcag cggcacagcg gtttaatgaa gctgaacaca atgggagcta tgacgacttc
    27121 cgcgacttta gccgtcgcca gccactgacc cagcaacaaa aggacgccgc tcgtcagcgt
    27181 tatcagtcgg cttctcctga gcagcgccag gcggctcgcg agaaaatgca gactaaccca
    27241 cagaaccagc agcgaagaga ggcggcacgt cagcgtattc agtccgcgtc gcctgagcag
    27301 cgccaggcgg ttcgcgagaa aatgcagact aacccgcaga atcaacagca aagagatgca
    27361 gcacgtcagc gtatccagtc tgcctcgcct gagcagcgtc aggtttttcg agagaaagtt
    27421 caggagagcc gcccgcagcg tctaaacgac agtaaccata ctgcccgatt gaataatgag
    27481 caacggtcag cggtacgcga acggttgtct gagcgtggag caaggagact ggaaaggtaa
    27541 attgcaggcg taaaaaagcg gcgttgttag ccgcttaaga ctgatgacaa acatcgaatc
    27601 gcccgatgcg caagcttatc gggcctacat aagccatgca atatattgaa tttgcatgat
    27661 tttgtaggtc ggataaggcg tttacgccgc atccggcatc aaccacgagc actttatcaa
    27721 caatctgaag cggccagaat agccgcttct taatttgcag gatgttccgg caaacgaaaa
    27781 attacttctt tttcgctttc gggttcggca ggtcggtaat gctaccttcg aacacttctg
    27841 ccgccaggcc cacagactcg tgcagagtcg ggtgcgcatg gatggtcagt gcgatgtctt
    27901 cagcatcaca acccatttcg attgccaggc cgatttcacc cagcagctcg ccgccgttag
    27961 taccgacaat cgcaccaccg atcacacggt gagattcttt gtcgaaaatc agcttggtca
    28021 taccgtctgc gcagtcagaa gcgatagcac gaccagaagc agcccacggg aaggtggcgg
    28081 tttcgtagct aatgcctttc tctttcgctt ctttctcagt cagacctacc catgcaactt
    28141 ctggttcggt ataggcgatg gacgggataa ctttcggatc gaagtaatgt tttttaccgg
    28201 cgataacttc agcggcaacg tgaccttcgt gaacaccttt gtgtgccagc attggctgac
    28261 cgacgatatc gccgatagca aagatgtgcg gtacgttggt acgcagctgt ttgtcaacgc
    28321 ggatgaaacc tcggtcgtca acttccacgc cagctttgcc tgcgtcgagg tttttaccgt
    28381 tcggcacacg accaatcgct accagcacgg cgtcgtaacg ctgcggttca gcgggtgctt
    28441 ttttgccttc catcgtcaca taaatgccgt cttctttcgc ttcaacggcg gtaactttgg
    28501 tttccagcat caggttgaat ttcttgctga tacgcttggt aaagacttta acgatgtctt
    28561 tatcagctgc ggggatgacc tggtcgaaca tttcaaccac gtcaatctgt gaacccagcg
    28621 cgtggtatac ggtgcccatt tccagaccga tgataccgcc acccattacc agcaggcgtt
    28681 ctggtacttc tttcagttcc agcgcgtcag tggagtccca gatacgcgga tcttcatgcg
    28741 gaataaacgg cagttgaatc gggcgagaac ccgctgcaat gatcgcgttg tcgaagttga
    28801 tcacggtttt accgttctca ccttcaactt ccagggtgtt agccccggta aatttaccca
    28861 gaccgttgac cactttgact ttgcggcctt tcgccatacc agccagacca ccggtcagct
    28921 gattgatcac tttctctttc caggtacgaa tcttgtcgat atcggttttc ggttcgccaa
    28981 agacgatacc gtgttcagcc agcgctttgg cttcttcgat aacttttgct acgtgcagca
    29041 gtgctttaga agggatacag ccgacgttca ggcaaacacc gccgagggtg ttgtagcgtt
    29101 ctacgattac ggtttccaga cctaaatcag cgcaacggaa ggcagcggag taacctgcgg
    29161 ggcctgcccc aagtaccacg acctgagttt tgatttcagt actcatcatg acctctatat
    29221 atttatctcc ggcgggtctg acgttttttt tatgacgccc attatccacc gggtcgttct
    29281 atccgtcggt attttacaaa attgttaaca atttttaaac aacaaacggc aaccgatttg
    29341 tctattcgct aataatctca atacattcat gagattacca gaaaagagcc ggccgttggg
    29401 ccggctcttt tatttacatc accagacggc gaatgtcaga cagcgtgttg ttaatgatgg
    29461 taatgaaacg ggcaccatca gcaccgtcga tcacgcggtg gtcgaaggag agagaaatcg
    29521 gcagcatcag acgcggcacg aactctttac cattccacac cggctccatc gcggacttgg
    29581 aaacgccgag gatagccact tccggcgcgt tcacaatcgg cgcgaagtgg gtagtaccca
    29641 ggccgccgat gctggagatg gtgaagcaac cgccctgcat ttcgcccgct gtcagcttac
    29701 cgtcacgcgc tttcttagaa atagtcatca gctcgcgaga cagctcgatg atgcctttct
    29761 tgttgacgtc tttgaatacc gggacaacca gaccgttcgg ggtatccacc gccacaccga
    29821 tgttgatgta tttcttcagg gtcagacgct gaccgtcttc cgacagcgaa ctgttgaagc
    29881 gaggcatctg ctcaagagct gcagcaacgg ctttcatgat gaagacaacc ggggtgatct
    29941 tcacatccag cttacgtttc gccgcttctt cgttctgctg tttacggaac gcttccagct
    30001 cggtgatatc ggttttgtcg aagtgagtaa catgcgggat catcacccag ttacggctca
    30061 ggttcgcacc agagattttc tggatgcggc ccagttccac ttcttcgatt tcaccaaact
    30121 tgctgaagtc caccttcggc cacggcagca tgcctgggat accgccgcca gttgccgccg
    30181 gagctgcttc tgcacgtttg atagcttctt tcacgtaagc ctgaacgtct tcgcgcagga
    30241 tacgaccttt acggccggtg cccttcactt tcgccaggtt aacgccaaac tcgcgtgcca
    30301 gacggcggat cagcggagtc gcgtgaacgt aagcgtcatt ttcagcaaat tcagatttgc
    30361 cttcggcttt tgcagccgga gctgctgccg gggcttcagc ttttgctgcc ggagccggcg
    30421 ctgccgcttc ctgtttcgca ggagctgccg caggcgctgc gccttcaact tcgaagatca
    30481 taatcagcga accagttttc actttatcgc caacgttgac tttcagttcc ttcacgacgc
    30541 ctgcaaacgg agccggaact tccatagaag ctttatcgcc ttctacggtg atcagtgact
    30601 gttcagcggc aactttgtcg cccactttca ccatcacttc ggtcacttca acttcgtcac
    30661 cgccgatatc cggaacgtta acttctttca cgccagctgc tggtgcaggg gccgctgccg
    30721 gagccgcttc ctgtttagcc gccggagctg ccgcgcctgc ttcacccgcg acttcgaaga
    30781 tcataatcag cgagccggta gacactttgt cacccacgtt cactttgatc tctttcacgg
    30841 tgcctgcaaa cggagcagga acttccatag aagccttgtc gccttctacg gtgatcagcg
    30901 actgttcagc ttcaacttta tcgccaactt tcaccaggat ttcggtcact tcaacttcgt
    30961 cgctgccgat atccggaacg ttaacgtctt ttgctgccgc agccgctggt gctgctgccg
    31021 gagctgcttc tttcttctct tctgcctgag caggtgcagc gtctgctgca ccgtcggcgg
    31081 aatcgaaaat cataatcagt gcgccggtct gggttttatc gccaacagag actttgatct
    31141 ctttaacgat acccgcctgc ggagatggta cttccataga ggctttgtcg ccttctacgg
    31201 tgatcagcga ctgttcggct tcaactttgt cgcccacttt gaccaggatc tcggtgattt
    31261 caacttcatc agccccgatg tccggtactt tgatttcgat agccattatt cttttacctc
    31321 ttacgccaga cgcgggttaa ctttatctgc atcgatgttg aatttggcga ttgcgtcagc
    31381 aaccactttc ttatcgattt cgccacgttt agccagttcg cccagcgccg caaccacgac
    31441 ataagaagca tcaacttcaa agtggtgacg caggttctca cggctgtcgg aacgaccgaa
    31501 gccatcagta cccagtacgc ggtagtcgtc agccggtacg taagtacgga cctgctcagc
    31561 gaacagtttc atatagtcgg tagatgccac tgccggagcg tcgttcatca cctgagcgat
    31621 atacggaacg cgcggagttt ccagcgggtg cagcatgttc cagcgttcac aatcctgacc
    31681 atcacgcgcc agttcggtga aggaagtcac gctataaacg tcagaaccta cgccgtaatc
    31741 tttcgccagg atctctgccg cttcacggac gtgacgcagg atagaaccgg agcccagcag
    31801 ctgaacttta cctttgctac cttcaatggt ttcgagtttg tagatacctt tacggatacc
    31861 ttcctcagca ccttccggca ttgccggcat gtggtagttt tcgttcagcg tagtgatgta
    31921 gtagtaaacg ttctcttgtt tttcaccata catacgctcc agaccatcat gcatgatgac
    31981 agcaacttcg taagcgtaag ccgggtcgta agagatacag ttcgggatag tcagcgactg
    32041 aatatggctg tgaccatctt cgtgctgcag accttcgccg ttcagggtgg tacgaccgga
    32101 agtaccgccg atcaggaagc cacgcgcttg ctggtcgcca gccgcccagc acagatcgcc
    32161 gatacgctgg aaaccgaaca tcgagtaata gatgtagaac gggatcatcg gcagattgtt
    32221 ggtgctgtaa gaggtcgccg ctgccagcca ggaacaacct gcgcccagct cgttgatccc
    32281 ttcctgcaga atctgacctt tctcgtcttc tttatagtaa gcaacctgct cgcggtcctg
    32341 cggggtgtac tgctgaccgt tcgggctgta aataccaatc tgacggaaca gaccttccat
    32401 accgaaagta cgcgcttcgt cggcgatgat cggtaccagc ctgtctttga tcgacttgtt
    32461 cttcagcatt acgttcaggg cacgaacgaa agcgatagtg gtagagatct ctttgctctg
    32521 ctcttccagc agcgcgccga agtcttgcag gctcggcagc tcaagcttct cggtgaagtt
    32581 cggctgacgg cttggcagat aaccgtgcag tttctgacgc tgagcgtgca gataggtatg
    32641 ctcttcagaa ccttccggga aggtgatgta cggcagtttt tcgatatctg catcagacac
    32701 cggcacattg aaacggtcgc ggatatgacg cacgccgtcc atgttcattt tcttaacctg
    32761 gtgcgcgatg tttttacctt cagccgcgtc gcccatgcca taacctttaa tggtatgagc
    32821 aaggattacc gtcgctttgc ctttggtttc ctgcgctttc ttgaatgcag cgtagatttt
    32881 cttcggatcg tgaccaccac ggttcagtgc ccagatctgc tcgtcagtcc agtctgcaac
    32941 cagtgctgcg gtttcaggat atttaccgaa gaagtgttcg cgaacatacg caccatcttt
    33001 cgatttgaag gtctggtaat cgccgtcaac ggtttcgttc atcagctgga tcagtttacc
    33061 gctggtatct ttacgcagca gttcgtccca acggctaccc cacatcactt tgatcacgtt
    33121 ccagccagca ccttcgaaga tgccttccag ttcgttgatg atcttgccgt taccggtgac
    33181 cgggccgtca agacgctgca ggttacagtt gataacgaag accagattat ccagtttttc
    33241 acgggtagcg atggtgatcg cacctttgga ttccggttcg tccatttcac cgtcgccgag
    33301 gaacgcgtaa acggtttgtt tagaggtatc tttcaggcca cggtgttcca gatatttcag
    33361 gaatttagcc tggtaaatag caccaatcgg acccagcccc atagatacgg tcgggaactg
    33421 ccagaattcc ggcatcagtt tcgggtgcgg ataggaagag aggccattgc cgtgaacttc
    33481 ctgacggaag ttatccagct gctcctgagt cagacgacct tccaggaaag cacgagcgta
    33541 aacgcctggg gagatgtggc cctggaagta aaccaggtcg ccgccatcct gctcgttgcg
    33601 tgcacggaag aagtggttaa agcacacatc ataaatggtt gcggaagact ggaaggacgc
    33661 catgtggccg cccagttcga ggtctttttt cgacgcacgc agcaccgtca tgatggcgtt
    33721 ccagcggata gctgaacgaa tacggcgttc cagttccaga ttacccggat actccggttg
    33781 ttcttcaacg gggatggtgt tgatgtagtt gctgataccg gtgcctgcag ctacgttgac
    33841 tccgcctttg cgggcttcag caagcagttg gtcgatcaga tactgagcac gctcaacacc
    33901 ttcttcacgg atgaccgatt cgatcgcctg gagccagtcg cgagtttcga tcggatccac
    33961 gtcatttggg aaacgttctg acatgggtta ttccttatct atctaataac gttgagtttt
    34021 ctggaacctg tcccattgaa ctctcgccgg aaagctcaat aagacaggtt ctacgtttag
    34081 ttgccgcgct tttatatgcg cttgatttac aacatcttct ggataatttt taccagaaaa
    34141 atcactaatt ctttcgttgc tccagacgac gcagagaacg ctcacggcgg ctctcttcac
    34201 ggcttctgtc gagcaaaatt tcttcgataa aggccagatg gcgatgcgat gcttcgcgcg
    34261 cttcttccgg cttaccggcc ataatcgctt caaatatgcg ggtgcggtga ctactcacca
    34321 gcggcagcat ctcgcgacgc gaatagagca attcgaagtt ctggcggaca ttctgggcca
    34381 acatcggctc catacacctt agcagatgaa gcagaaccac attgtgggcc gcttcggtga
    34441 cggcaatctg atactggagt acggcgtttg attccgcgtc cagatcgcca gactgctgcg
    34501 ccagctctat ggcatggtgg agttcacgga tgcgttcctt gtcttcatcg gtactacgca
    34561 gcgcggcgta ataagcggcg ataccttcca gggcgtgtcg tgtttcgagc aagtcatact
    34621 gtgattcagg atggtcggag agcagctcca ccagcggatc gctgaagctt tgccataggc
    34681 tgctctggac aaaagtgccg ccaccctggc gacgaagcaa caagcccttc gcttcgagac
    34741 gttgaatcgc ctcacgcaat gagggacggg agacgtcaaa ctgttttgcc agttcgcgtt
    34801 ccggtgggag tttttcgccc gggcggagag tgccttcgag gatcaaaaac tccagttgct
    34861 gctcaatcac atcggagagt tttggttggc ggattttgct gtaggccatg agttcctgtc
    34921 ttaagccact tgccgaagtc aattggtctt accaatttca tgtctgtgac gctaaagtaa
    34981 caaagtattc accttatgtc catacaggtt ttgattgaaa tcatgaaact gtgcacattt
    35041 taacaacttg acatatataa cgtttcaaag ttgtaactat gcacaaatgt taaatttac
//
LOCUS       DAZGJT010000042        34561 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZGJT010000042 DAZGJT010000000
VERSION     DAZGJT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.042"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(53..778)
                     /gene="phoU"
                     /locus_tag="ACHKFK_002252"
     CDS             complement(53..778)
                     /gene="phoU"
                     /locus_tag="ACHKFK_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO9052588.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDRDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(793..1566)
                     /gene="pstB"
                     /locus_tag="ACHKFK_002253"
     CDS             complement(793..1566)
                     /gene="pstB"
                     /locus_tag="ACHKFK_002253"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO9052589.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1657..2547)
                     /gene="pstA"
                     /locus_tag="ACHKFK_002254"
     CDS             complement(1657..2547)
                     /gene="pstA"
                     /locus_tag="ACHKFK_002254"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO9052590.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMIAAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2547..3506)
                     /gene="pstC"
                     /locus_tag="ACHKFK_002255"
     CDS             complement(2547..3506)
                     /gene="pstC"
                     /locus_tag="ACHKFK_002255"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO9052591.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3593..4633)
                     /gene="pstS"
                     /locus_tag="ACHKFK_002256"
     CDS             complement(3593..4633)
                     /gene="pstS"
                     /locus_tag="ACHKFK_002256"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO9052592.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4947..6776)
                     /gene="glmS"
                     /locus_tag="ACHKFK_002257"
     CDS             complement(4947..6776)
                     /gene="glmS"
                     /locus_tag="ACHKFK_002257"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO9052593.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGYMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6937..8307)
                     /gene="glmU"
                     /locus_tag="ACHKFK_002258"
     CDS             complement(6937..8307)
                     /gene="glmU"
                     /locus_tag="ACHKFK_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO9052594.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKQQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGNRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8658..9077)
                     /gene="atpC"
                     /locus_tag="ACHKFK_002259"
     CDS             complement(8658..9077)
                     /gene="atpC"
                     /locus_tag="ACHKFK_002259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO9052595.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9098..10480)
                     /gene="atpD"
                     /locus_tag="ACHKFK_002260"
     CDS             complement(9098..10480)
                     /gene="atpD"
                     /locus_tag="ACHKFK_002260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO9052596.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10507..11370)
                     /gene="atpG"
                     /locus_tag="ACHKFK_002261"
     CDS             complement(10507..11370)
                     /gene="atpG"
                     /locus_tag="ACHKFK_002261"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO9052597.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11421..12962)
                     /gene="atpA"
                     /locus_tag="ACHKFK_002262"
     CDS             complement(11421..12962)
                     /gene="atpA"
                     /locus_tag="ACHKFK_002262"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO9052598.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12975..13508)
                     /gene="atpH"
                     /locus_tag="ACHKFK_002263"
     CDS             complement(12975..13508)
                     /gene="atpH"
                     /locus_tag="ACHKFK_002263"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO9052599.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13523..13993)
                     /gene="atpF"
                     /locus_tag="ACHKFK_002264"
     CDS             complement(13523..13993)
                     /gene="atpF"
                     /locus_tag="ACHKFK_002264"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO9052600.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14055..14294)
                     /gene="atpE"
                     /locus_tag="ACHKFK_002265"
     CDS             complement(14055..14294)
                     /gene="atpE"
                     /locus_tag="ACHKFK_002265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO9052601.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14341..15156)
                     /gene="atpB"
                     /locus_tag="ACHKFK_002266"
     CDS             complement(14341..15156)
                     /gene="atpB"
                     /locus_tag="ACHKFK_002266"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO9052602.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPHNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15165..15545)
                     /gene="atpI"
                     /locus_tag="ACHKFK_002267"
     CDS             complement(15165..15545)
                     /gene="atpI"
                     /locus_tag="ACHKFK_002267"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO9052603.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(16162..16785)
                     /gene="rsmG"
                     /locus_tag="ACHKFK_002268"
     CDS             complement(16162..16785)
                     /gene="rsmG"
                     /locus_tag="ACHKFK_002268"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO9052604.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(16849..18738)
                     /gene="mnmG"
                     /locus_tag="ACHKFK_002269"
     CDS             complement(16849..18738)
                     /gene="mnmG"
                     /locus_tag="ACHKFK_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO9052605.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGYNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(19117..19560)
                     /gene="mioC"
                     /locus_tag="ACHKFK_002270"
     CDS             complement(19117..19560)
                     /gene="mioC"
                     /locus_tag="ACHKFK_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO9052606.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEETGLTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKIEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(19650..20108)
                     /gene="asnC"
                     /locus_tag="ACHKFK_002271"
     CDS             complement(19650..20108)
                     /gene="asnC"
                     /locus_tag="ACHKFK_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO9052607.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            20260..21252
                     /gene="asnA"
                     /locus_tag="ACHKFK_002272"
     CDS             20260..21252
                     /gene="asnA"
                     /locus_tag="ACHKFK_002272"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO9052608.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(21257..22708)
                     /gene="viaA"
                     /locus_tag="ACHKFK_002273"
     CDS             complement(21257..22708)
                     /gene="viaA"
                     /locus_tag="ACHKFK_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO9052609.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKMIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(22702..24198)
                     /gene="ravA"
                     /locus_tag="ACHKFK_002274"
     CDS             complement(22702..24198)
                     /gene="ravA"
                     /locus_tag="ACHKFK_002274"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO9052610.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGALVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPASLQITDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRMGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPSEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            24421..26289
                     /gene="kup"
                     /locus_tag="ACHKFK_002275"
     CDS             24421..26289
                     /gene="kup"
                     /locus_tag="ACHKFK_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO9052611.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGGEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            26456..26875
                     /gene="rbsD"
                     /locus_tag="ACHKFK_002276"
     CDS             26456..26875
                     /gene="rbsD"
                     /locus_tag="ACHKFK_002276"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO9052612.1"
                     /translation="MKKGTVLNSDISSVISLLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAVIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            26883..28388
                     /gene="rbsA"
                     /locus_tag="ACHKFK_002277"
     CDS             26883..28388
                     /gene="rbsA"
                     /locus_tag="ACHKFK_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO9052613.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            28393..29358
                     /gene="rbsC"
                     /locus_tag="ACHKFK_002278"
     CDS             28393..29358
                     /gene="rbsC"
                     /locus_tag="ACHKFK_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO9052614.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            29383..30273
                     /gene="rbsB"
                     /locus_tag="ACHKFK_002279"
     CDS             29383..30273
                     /gene="rbsB"
                     /locus_tag="ACHKFK_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO9052615.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            30399..31328
                     /gene="rbsK"
                     /locus_tag="ACHKFK_002280"
     CDS             30399..31328
                     /gene="rbsK"
                     /locus_tag="ACHKFK_002280"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO9052616.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDISPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKTLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            31332..32324
                     /gene="rbsR"
                     /locus_tag="ACHKFK_002281"
     CDS             31332..32324
                     /gene="rbsR"
                     /locus_tag="ACHKFK_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO9052617.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(32290..33717)
                     /gene="mdtD"
                     /locus_tag="ACHKFK_002282"
     CDS             complement(32290..33717)
                     /gene="mdtD"
                     /locus_tag="ACHKFK_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO9052618.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDTTGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIWLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(33740..34432)
                     /gene="yieP"
                     /locus_tag="ACHKFK_002283"
     CDS             complement(33740..34432)
                     /gene="yieP"
                     /locus_tag="ACHKFK_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO9052619.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAIKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVIRICLEPQACLLAAKVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         8334 a         8911 c         8814 g         8502 t
ORIGIN      
        1 agttatatat aacaaatccc aataattgaa ttattgggat ttacttggtg aattatttat
       61 cgctatcttt ttctgccagc agtttatcga gctcgtcacc accgacgtga cggaaatcct
      121 gccccttcac gtagtagaag ataaactcac aaatattctg gcagcggtcg ccaatacgtt
      181 cgatagaacg cgcacagaat aacgcggtaa gtacgctcgg aatggtacgc gaatcttcca
      241 tcatgtaggt catcagttga cgaacaatac cttcgtactc ctgatcgact tttttatctt
      301 cacgataaat acgtaccgct tcgtcaatgt ccatccgcgc gaacgcgtcc agcacgtcgt
      361 gcagcatctg gatggtatga cggcccagcg actccagact taccagcaac ggctgatgct
      421 gctgggagaa tttctccagc gcagtacggc agattttgtc cgccacgtcg ccaatacgct
      481 ccagctcggc aatggtttta ctgatcacca tcaccagtcg caggtcgctc gccgtcggct
      541 ggcgtttggc gataatgcgc acgcaggctt catcgatcgc cacttccatc atgttgacgt
      601 ttttgtcgcc ttcgatgacg cgcttcgcca gatctctgtc ctggttatgc atcgcggtga
      661 ttgcatcaga aagctgctgc tccaccatgc cgcccatggt catcacctgc gtgcggatgc
      721 tttccagttc ggcgttgaac tggccggaaa tatgtttatt aagattgaga ctgtccataa
      781 cgcactcctg aatcaaccgt aacgaccggt gatgtagtct tctgtttgtt tcttcgctgg
      841 cttggtgaac agatcgtccg tgttgctgaa ctcaatcaat tcgcccaggt acataaacgc
      901 cgtgtggtcg gaacaacgcg cagcctgctg catgttgtgg gtgacgatca ccacggtgta
      961 atcctgcttc agttcggtga tcagctcttc aatacgcccg gtagagatgg ggtcgagtgc
     1021 cgaacacggt tcgtcgagca gcagcacttc cgggcgaatg gcgataccac gcgcaataca
     1081 caaacgctgt tgctgaccac cagagagaga gtaaccgctc tggtgcaatt tatctttggt
     1141 ttcgttccac aatgcggctt tggtcaatgc ccactgcacg cgctcgtcca tgtcagcacg
     1201 ggagagcttc tcaaacagac gcacgccaaa agcgatgttg tcgtagatgg acatcgggaa
     1261 cggcgtcggt ttctggaaca ccatgcccac tttcgcacgc agcagggcga tatcctgaga
     1321 gttggtcagg atgttgtcgc catcgagcag aatttcacct tccgcacgct gttccgggta
     1381 cagttcaaac attttgttga aggtacgcag cagcgttgat ttaccgcagc cggacggccc
     1441 gataaacgcc gtaacctggt ttttggcgat atccaggttg atgtttttca gggcatggaa
     1501 tttgccgtag tagaagttca aattacgaac ctgaatttta ctcggggcag tttcaaccat
     1561 actcattgca atcttttcct catcgtgcct gatgcacttc gcgaatcagg cttaccgtag
     1621 gccggataag gcgtttacgc cgcatccagc aaagtgttaa ccgtgtttat tcttcgcaaa
     1681 aacaacgcgc gccagaatgt tcagcagcag tacgcacagg gtaatgatca acaccccggc
     1741 ccaggccagt tgctgccatt ccgcaaacgg gctcatcgca aacttaaaga tcgtcaccgg
     1801 caggttggcg atcggctgca tcatgtccgt gctccagaac tggttggaga gcgcagtaaa
     1861 cagcagcggt gcggtttcac cagcaatacg ggcaatcgcc agcaggatac cggtcatgat
     1921 cccggacacc gacgctttca gcgtgatcgc ggcgatcatc ttccacttcg gcgtacccag
     1981 cgcataagcc gcttcacgca ggctgtccgg caccagtttc agcatgttct cggtggtgcg
     2041 gataacaatc ggcacctgca acagcgccag cgcaatcacg cccgcccagc cggagaagtg
     2101 ctccatctgc gccaccacga tggtgtaaac aaaaagacca accacaatcg acggtgcgga
     2161 gagcaaaatg tcgttaatga agcgaatcac ttctgccagc caggatttac gaccatattc
     2221 agccagataa atccccgcca taatgcccag cggcgtaccg aataccgtgg cccacaaaat
     2281 taacaggccg ctacccgcca gggcgttcgc cagaccaccg ccttccgtat ttggcggcgg
     2341 cgtcatttcg gtgaacagcg ccagcgacat accatcgata ccgcgagtga tggtggacat
     2401 taaaatccag atcagccaga acagaccgaa ggccatcgtc gccatcgaga gcgtcagcgc
     2461 aatacggttc ttaaggcggc gacgcgcctg cattttacgg cgggattcag ccagcgccgc
     2521 agtggtttgc atttcaacca tagccattag cgtgccccct cattcttagc caggcgcata
     2581 atcataaact tcgatgcggc gaggacgatg aaggtaatca caaacaggat caggcccagt
     2641 tccatcagtg cagcaacgtg cagacccgac tctgcttccg caaattcgtt ggccagcgca
     2701 gaggtgatac tgttgcccgg catgtatagc gaggcgctgt cgagttggta ggtgttaccg
     2761 atgataaagg tcaccgccat ggtttcaccg agcgcgcgcc ccagccccaa cataatgccg
     2821 ccgataacac catttttggt gaacggaaga acgatacgcc agataacttc ccaggtggtg
     2881 cagccaatac cgtaggccga ctctttcatc atcaccgggg tttgttcgaa tacatcgcgc
     2941 attaccgccg caatgtacgg aataatcatg atggcgagga tcacgcctgc cgcgaggata
     3001 ccgataccaa aggccgggcc ggagaacagc gcgccgacaa tcgggatatt cgacatgata
     3061 ttgccgaccg gctcctggaa gtaaacggcg aacagcggcg caaagataaa caggccccac
     3121 atgccgtaaa cgatacttgg aatggcagcc agcagctcaa tggcgatacc cagcgggcgt
     3181 ttcagccagc caggcgcaag ctcggtcagg aacagggcga taccaaaact caccgggacg
     3241 gcgatcagca gcgcgataaa cgaggtcacc agcgtaccgt agatcggcac cagcgccccg
     3301 tagatatcgt tcggcgcatc ccactctttg gtccacagga aagccagacc aaatttctga
     3361 atgctcggcc aggatgagat gatcagagag acaataatgc cacccaacat caatagcaca
     3421 atcagcgccg ccagttttac cagcacgctg aaaattatgt cgcccttttt acccggtggg
     3481 ttaaaagcag gcttggttgc agccataagt tactcttcag ttaaacgcgt ttacaaagtc
     3541 attttgtaat gccggatgcg gcgtaaaaca ccgtacccgg cctggagttt tattagtaca
     3601 gcggcttgcc gctactgtct ttaatattgg tcttccacgc agcgcgaacc tgttcaacta
     3661 cgctatccgg caggctggca taatccaggt cgttcgcctg tttagccccg gttttgtatg
     3721 cccagtcgaa gaacttcagc acttcggtgc cttgttctgg tttcttctga tctttatgga
     3781 tcagaatgaa cgtggtagag gtaatcggcc atgcatcttc acctttctgg ttggtcagat
     3841 cctgagcgaa tgttttgctc cagtctgcgc cttttgctgc attagcgaag ttttcttcgg
     3901 tcggactaac cggtttgcca tctgcggaga tcagtttggt gtacgccagg ttgttctgct
     3961 tcgcgtaagc atattcaacg taaccaattg cacccggcag acgctgaacg aacgcggcga
     4021 taccgtcgtt acctttaccg cccagaccga tcggccattt tacggtagag ccagtaccaa
     4081 cgttattttt ccactcttcg ttcactttcg ccaggtagct ggtgaagacg aaggaagtcc
     4141 cggagccatc tgcgcggcgt actacagcaa tgttctgtga aggcagtttc agacccggat
     4201 tcagtttggc gatggcttca tcatcccact tcttgatttt gcccaggtag atgtcgccga
     4261 gggttttacc atccagcacc agttcgccag acttcagacc tggaatatta accgccagca
     4321 ccacgccgcc aatcacggtc gggaactgga acaggccttc ctgagccagt ttttcgtcag
     4381 acagcggcgc gtcagaagca ccaaaatcaa cggtattggc gatgatctgt tttacaccac
     4441 cagaagaacc gataccctgg tagttaactt tattaccggt ttctttctgg taagtgtcag
     4501 cccatttggc atacaccggc gcagggaagg ttgcacctgc acctgtcagg cttgcttctg
     4561 caaacacaga gaaagcgctc atcgataagg tcgcggcgac aacagttgcg acggtggtac
     4621 gcataacttt cataatgtct cctgggagga ttcataaagc attgtttgtt ggctacgaga
     4681 agcaaaatag gacaaacagg tgacagttat atgtaaggtt tatgacagtt ttatgacaga
     4741 gagagaaagt ctttagtctg atttaaataa gcgttgatat tcagttaatt acaaatatta
     4801 ataacgaaga gatgacaaaa aaattttcat tctgtgacag agaaaaagta gccgaagatg
     4861 acggtttgtc acatggagtt ggcaggatgt ttgattaaaa acataacagg aagaaaaatg
     4921 ccccgcttac gcagggcatc catttattac tcaaccgtta ccgattttgc caggttacgc
     4981 ggctggtcaa cgtcggtgcc tttgatcagc gcaacgtggt aagccagtag ttgcagcgga
     5041 acggtgtaga agatcggtgc aatcacctct tccacatgcg gcatctcaat gatgtgcatg
     5101 ttgtcactgc tcacaaaacc cgcatcctga tcggcaaaga catacaactg accgccacgc
     5161 gcgcgaactt cttcaatgtt ggatttaagt ttttccagca attcgttgtt cggtgcaacg
     5221 acgataaccg gcatatcggc atcaatcaac gccagcggac cgtgtttcag ttcgccagca
     5281 gcgtaggctt cagcgtgaat gtaagagatc tctttcagct tcaatgcacc ttccagcgcg
     5341 attgggtact gatcgccacg gcccaggaac agcgcgtgat gtttgtcaga gaaatcttct
     5401 gccagcgctt caatgcgttt gtcctgagac agcatctgct caatacggct cggcagcgcc
     5461 tgcagaccat gcacgatgtc atgttcaatg gaggcatcca gacctttcag gcgagacagt
     5521 ttcgccacca gcatcaacag cacagttaac tgggtggtga atgctttggt ggaggccacg
     5581 ccaatttccg taccggcgtt ggtcatcagc gccagatcgg attcgcgcac cagagaagaa
     5641 cccggaacgt tacagattgc cagcgaacca aggtaaccca gctctttcga cagacgcagg
     5701 cccgccaggg tatccgcggt ttcgccagac tgtgacaagg tgatcatcag gctgttacga
     5761 cgcacggcag atttgcgata gcggaattca gaggcgattt cgacgtcgca cggaatacct
     5821 gccagcgatt caaaccagta gcgggaaacc ataccggagt tataagaagt accacaggcg
     5881 aggatctgaa tatgctcaac cttcgacagc agttcatcgg cgttaggtcc cagctcgctt
     5941 aaatcaacct gaccgtggct gatgcgcccg gtaagggtgt ttttgatcgc gttcggctgt
     6001 tcgtagatct ctttctgcat gtagtggcgg taaatacctt tatcgcccgc gtcatattgc
     6061 agattggatt cgatatcctg acgttttact tccgcgccag ttttatcgaa gatgtttacc
     6121 gaacggcgag tgatttccgc aatatcgccc tcttcaagga agataaaacg acgagtcact
     6181 ggcaacagcg ccagctggtc agaagcgata aagttttcgc ccatacccag gccaatcacc
     6241 agcggactac cagaacgtgc cgccagcagg gtatccggat gacgggagtc catgatcact
     6301 gtaccgtacg caccacgcag ttgcgggata gcacgcagaa ctgcctcacg cagagtaccg
     6361 ccttgtttca gctcccagtt caccaaatgt gcaatcactt cggtgtcggt ttcagaaacg
     6421 aaggtatagc cacgcgcttt taactcttca cgcagcggtt catggttttc gatgatgccg
     6481 ttatgcacca ccacaatgtg ttcagaaaca tgcggatgcg cgttagcttc tgaaggttca
     6541 ccgtgggtcg cccagcgagt atgagcaata ccggtgccgc catgcagagg atgttcttcc
     6601 gctgcctgag ccagcatctg gactttaccg aggcgacgca ggcgggtcat ataaccttct
     6661 gcatcaacaa cggccagacc ggcagagtca tatccgcggt attccagacg acgtaaacct
     6721 tcaagaagga tttctgctac atcacgttgc gcgatcgcgc caacaattcc acacatagtt
     6781 tttgattccg atttatatcg ttgtcggtca acctgtatgc ccgtttttcg ggcgccccga
     6841 gccttgtaga gagtgggggt atttttatag ttactgcttg tgggcgggag attatgttat
     6901 ctcctcatcc catatgaccg gattacccgg ccagaattac tttttcttta ccggacgacg
     6961 ccagccttct ttttgagtct gcggcacacg gctgatagcc agggcatttt cgccgacatt
     7021 acgcgtcaca gttgtaccag cagcaatggt cgcgcctttg cctactgtta ccggggccac
     7081 cagctgagta tcggatccaa caaacacatc gtcgccgata atagtcttaa atttattcgc
     7141 accatcgtag ttgcaggtaa tggttcccgc gccgatgtta acgttatcgc caatttccgc
     7201 atcgcccagg taagtcagat gaccagcttt cgagccttta cccagacgcg cttttttcat
     7261 ctcaacgaag ttgccgacgt gtgcgccttc cagcaactca gcaccaggac gcagacgggc
     7321 aaacgggcca atggtacagg ccgctgccag attcgcatct tccacaacgg tatacgggct
     7381 aatttcgcaa tcatcgccaa tcacgctgtt tttaatcaca caaccggtgc cgattttcac
     7441 acgattaccg agagtcacgt tgccctcgat gataacgtta gtatcaattt caacatcgcg
     7501 cccgtgagta agcgtaccgc gcagatcaaa acgcgccgga tcgcgcagca taacgcctgc
     7561 taacagtagt ttttcagcct gttcggactg gtaaacgcgc tccagtcggg agagttgcag
     7621 gcggttattc acgccttcta cttcgcttaa acgttgcgga tgaacggcga cgatttcacg
     7681 cccttcctga tacgccagcg caataatgtc ggtgatgtag tattcaccct gagcgttatt
     7741 gttggtcagc ttcgccagcc agcgtttcat atctgcgccg ttggcaatca gaatgccggt
     7801 gttgatctcc tgaatctgac gctgctcgtc ggtggcgtct ttgtgctcaa caatgccggt
     7861 aactttgccg ttttcacggg tgatacgtcc ataaccggtc ggatcatcca gttttaccgt
     7921 cagcagacca atgccaccct gctgtttagc atcacgcagg cgctggagtg tttcgacaga
     7981 gatcagcggc acgtcgccgt agagcattaa aatgtcttca tcatcggcaa agaaaggtgc
     8041 ggcctgctgc atcgcatgac ctgtacccag ctgttctgcc tgaagcaccc agttcaggtt
     8101 gtcatctttc agcgcctgtt taagcagatc gccgccgtga ccgtaaacca ggtgaacgtg
     8161 cgctgcgcct aattcattcg cagcatcaat gacatgctga accatcgctt tcccggcaag
     8221 ggtatgcagc actttcggaa gatcggaata catgcgcgtg cctttgcctg cggcaaggat
     8281 cactacgctc atagcattat tcaacatacg cgtcctgact gtaatttgag aacgaattta
     8341 aaccgcttca ccttgaaaaa actacatttt tttcatcgtg aaatggacag aggataaatt
     8401 gttcaataac ggattatccc cgctgacgaa agcgccattt tcgaccatcg tcacgccttt
     8461 tgtctgcaga aaattaaggc agtaccttca tttaagcgtc aggtggatgt ttttgctctt
     8521 atttcgatca atgaataaac gggaaaacag ggtgttttat atcagcagga tctatgtgaa
     8581 cgctcttcag gacgggtcaa cgcgtaaaaa aaagccagcc tgttaccaga ctggcttttg
     8641 tgcttttcaa gccggtgtta catcgctttt ttggtcaact cgataacgcg cagctgcgcg
     8701 atcgctttgg ccagttccgc agacgcctga gcgtaatcta cgtcgccgtg agagctgcta
     8761 atgtgctctt cagccttacg tttcgcttcc atggctcgcg cttcgtcgag atcctggccg
     8821 cgaattgcgg tgtcggccag aacggtcacg ttgccaggct gcacttcaag aatgccgcca
     8881 gacagataga taaactcttc gtgaccgtgc tgtttcacga tgcgaatcat accaggctta
     8941 atggcggtga gcagcggtgc gtggccaggg tagatcccca gttcaccttc gctacccgtt
     9001 acctggattt tctcgaccag accagagaac atttgttgct ctgcgctgac gacgtccagg
     9061 tggtaagtca ttgccatatc accctccgat taaggcgtta aagttttttg gctttttcca
     9121 cagcttcttc gatggaaccg accatgtaga acgcctgctc cggcaggtga tcgtattcgc
     9181 cttccatgat gcctttaaag ccacggatgg tgtctttcag ggagacgtat ttacccggag
     9241 aaccggtgaa tacttctgcc acgaagaacg gctgggacag gaagcgctgg atcttacgag
     9301 cacgcgctac caccagtttg tcttcttcag acagttcatc catacccagg atggcgataa
     9361 tgtctttcag ttcctgataa cgttgcagga tggactgaac gccacgcgcg gtgtcgtagt
     9421 gttcctgacc aaccaccagc gggtccagct gacggctggt ggagtccagc gggtcaacgg
     9481 ccgggtagat acccagagac gcgatctgac ggctcagtac cacggttgcg tcaaggtgcg
     9541 caaaggtggt tgccggagac gggtcagtca agtcatccgc aggtacgtat actgcctgta
     9601 cggaggtgat agaaccggtt ttggtggagg tgatacgttc ctgcagaacg cccatctctt
     9661 ccgccagggt cggctgataa cctaccgctg aaggcatacg gcccagcagt gcggatactt
     9721 ccgtaccggc cagggtgtaa cgatagatgt tgtcaacgaa cagcagaacg tcacgacctt
     9781 cgtcacggaa tttctcagcc atggtcagac cggtcagagc aacgcgcaga cggtttcccg
     9841 gcggctcgtt catctggcca tacaccaggg atactttgtc gataacgttg gagtcggtca
     9901 tttcgtggta gaagtcgtta ccctcacgag tacgttcacc tacgcccgca aacacagagt
     9961 aaccggagtg ctcgatcgcg atgttacgaa taagctccat catgtttacg gttttaccta
    10021 cacccgcacc accgaacaga ccaactttac cgcccttagc gaacggacac atcaggtcga
    10081 taactttgat accggtttcc agcagttcct gagagtttga cagctcttcg taggaaggtg
    10141 ctgcgcggtg aatcgcccaa cgctcttctt caccgatctc gcctttcatg tcgaccggtt
    10201 cacccagtac gttcatgata cggcccagag tcgctttacc taccggaact tcgatcgggt
    10261 gttcgaggtc ttttacatcc agaccgcgac gcagaccgtc ggaggaaccc attgcgatgg
    10321 tacgcacgat accgccgccg agctgctgct gaacttccag caccagacgc tcattaccat
    10381 tttgcacctc aagagcatcg tacacgcgcg gtacggcatc ctgagggaat tcgacgtcaa
    10441 ctacggcgcc gattacctgg acaatctttc cagtagccat cttaaatcct ctacgaaata
    10501 acctgtttaa accgcggcgg cccccgagac gatctcggtg agttcctgag taatgctggc
    10561 ctgacgagct ttgttgtata ccaactgcag ctctttaatc aggctgccgc cattgtcggt
    10621 cgcggctttc atcgccacca tacgggcggc ctgctcgctg gccaggtttt caaccacgcc
    10681 ctgataaacc tgagattcga cataacgacg cagcagggta tccagcaacg ccttcggatc
    10741 gggttcgtac aggtaatccc aggatttatg tttcagatca tcatcatctg atgccggtaa
    10801 cggcagcagc tggctgatgg tcggaacctg agacatggtg ttaataaatt tgttgctgac
    10861 aatgtaaagc ttgtccagac ggccttcgtc gtaggcctgc aacatcactt ttaccggacc
    10921 gatcagttcg gacagggaag ggttatcccc catgccggtg acctgggcaa caacattgcc
    10981 gcccacggag ttgaagaacg acacgccttt cgagccgatc attgcgaggt cgcattgaac
    11041 gcctttgtcg gtccaggtct tcatttccgc cagcagtttt ttgaacaggt taatgttcaa
    11101 accaccgcac aaaccacggt cggtcgacac caccaggtag cccacgcgtt taacgtcgcg
    11161 gtcttccagg taagggtgct tatattccag attaccgtgt gcaaggtgac caatcacttt
    11221 gcgcatggtt tctgcataag gacggctggc cgccatgcga tcctgcgatt tacgcatttt
    11281 ggaagcggcg accatctcca tcgctttagt gatcttttgc gtgttctgga cgcttgcgat
    11341 cttactacgt atctcttttg cgccggccat gagcttctcc tcaatgcctt gcggcctgcc
    11401 ctaaggcaag ccgccagacg ttaccaggat tgggttgctt tgaaggaatc gaggatgcct
    11461 ttcagcttgc cttcgatttc gtcgttgtag ccaccggtct ggttgatctc ttgcatcaac
    11521 ggagcgtgat cacggtcgac gtaagccagc agagcggctt cgaagctgcc gattttcgac
    11581 agttcaacat ccgccaggta accacgttct gctgcgaaca gaaccagaga ctgttgcgct
    11641 acagacatcg gcgcatactg tttctgtttc agcagttcgg tcactttctg accgtggtca
    11701 agctgcttac gtgttgcatc gtcaaggtcg gatgcaaact gagagaacgc tgccagttca
    11761 cgatactgtg ccagagcggt acggatacca ccggacagtt ttttcatgat cttggtctgt
    11821 gctgcaccac caacacggga tacggaaata cccgggttaa ccgcaggacg aataccggcg
    11881 ttgaacaggt tggtttccag gaagatctga ccatcggtaa tggagattac gttggtcgga
    11941 acgaacgcag aaacgtcacc cgcctgagtt tcgataatcg gcagcgcagt cagagaaccg
    12001 gttttccctt tcacttcacc tttggtgaaa gcttcaacgt attcggcgtt aacacgcgca
    12061 gcacgctcca gcagacgaga gtggaggtag aatacgtcgc ccgggaatgc ttcacgtcct
    12121 ggcggacgac ggagcagcag ggagatctga cggtaagcaa cagcctgttt agacaggtca
    12181 tcgtaaatga tcagcgcatc ttcaccgcgg tcacggaagt attcacccat tgcgcaacca
    12241 gcgtacggtg ccaggtattg cagtgctgcg gattcagacg cggttgctac cacaacgatg
    12301 gtgttagcca gtgcgccgtg ctcttccagt ttacgtacca cgttagaaat ggtggacgct
    12361 ttctggccga tagcgacata gatacatttg ataccggaat cgcgctggtt gatgatggca
    12421 tcgatagcca gtgcggtttt accggtctga cggtcaccga tgatcaattc acgctgacca
    12481 cgaccgattg ggatcatgga gtcaacggct ttataaccgg tctgtaccgg ctgatctacg
    12541 gactgacgtt cgataacgcc cggagcgatt gcttctacag cagagaagcc gtcgtgatcc
    12601 agcggacctt taccgtcgat tggtgcaccc agagtgttaa ccacacggcc cagcaggcca
    12661 cggccaaccg gaacttccag gatacggcca gtacacttaa ctttcatgcc ttcggcaagg
    12721 tcagcgtacg gacccataac aaccgcacct acagagtcgc gctcgaggtt cagtgcgata
    12781 gcgtaacggt tacccggcag ggagatcatt tcaccctgca tacaatcggc caggccgtga
    12841 atgcggataa caccgtcact tacagaaaca atagtacctt cgttgtgagc ttcactcaca
    12901 acattgaact gagcaatgcg ctgcttgatc agttcgctga tttcggtgga attcagttgc
    12961 atgctccagt ccccttaaga ctgcaagacg tctgcaaggc gctcaagacg accgcgtacg
    13021 ctgccatcaa tgaccatatc acccgctcgg atgataacgc ctgccattac agacttatcg
    13081 attttgcaat tcagcttaac tttgcgtgac agacgttttt ccatcgcagc agaaattttc
    13141 gcgagctgtt gttcactcaa cgcggcagcg gaaatgacgt ctacctcagc ggtagcctca
    13201 ctcacagcac gcaggtgaat aaactgctcc agaacatccg ggagcgcgtt aagacgacca
    13261 ttttcggcca ttacccgaat caggttctga ccgttttcgt ccagttgctc accacaaact
    13321 gcgataaacg actcggcgag cgtttctggc gcaagcgcgc cagagagaag ctctgccatt
    13381 tgttcgtttt tggttacctc ggcggcaaac gccagcatgt cctgccagcg ttctacactt
    13441 tggtgttcga cggcaaagtc aaaagctgct ttggcgtagg ggcgagctac cgtaataaat
    13501 tcagacatca gcccctccct ccttacagtt cagcgacaag tttatccacg atgtcgctgt
    13561 tagcagcttc atccacggaa cgttcgatga tcttctcggc gccagcaaca gccaggatag
    13621 caacttgctt acgcagctct tcacgggcac gtttacgctc ggcttcaatt tccgcctgcg
    13681 cctgggccac gattttagta cgttcctgtt ctgcctcagc tttcgcttcg tccagaatct
    13741 gcgagcggcg tttgttagcc tgctcgatga ttacctgggc ttccgctttc gcttttttca
    13801 gctggtcggt cgcgctggcc tttgcaaggt caaggtcctt atgtgctcgt tctgcggaag
    13861 caaggccgtc agcaatttct ttttgacgtt tttcgatggc tgccattaat ggcggccata
    13921 cgtacttcat gcagaacaga acgaacagga caaacgcgat ggcctggccg aggattgttg
    13981 cgttaagatt cacagcacaa tgcctctatt tagttaacgt tctgatattg ctctttaaat
    14041 aaaagcaacg cttactacgc gacagcgaac atcacgtaca gacccagacc tacagcgatc
    14101 atcgggatag catccaccag acccataacg ataaagaact gagtacgcag cagaggaatc
    14161 agatcaggtt gacgcgctgc gccttccagg aatttacccc cgaggatgcc gataccgatc
    14221 gcagcaccga ttgccgccag acccatcatc acagcggcag ccatgtacag cagatccata
    14281 ttcaggtttt ccatgacagt ctccagtttg tttcagttaa aacgtagtag tgttggtaaa
    14341 ttaatgttct tcagacgcca tcgacagata gacgatcgtc agaaccatga agatgaaggc
    14401 ttgcagcgta atgatcagga tgtggaaaat ggcccacggc acattcagga tccactgtga
    14461 ccaccacggc aacagaccag caatcagaat gaaaatcagc tcaccggcat acatgttacc
    14521 gaacagtcgc aaaccgagtg aaactggttt ggacagcagg cttacccctt caaggattaa
    14581 gttgacagga atgaacgccc agtgattgaa cggctgcagc gtcaactctt tcgtgaagcc
    14641 gccgatgcct ttcattttga tgctgtagaa cagaatcagg ataaatacgc ccagtgccat
    14701 agacagcgtt acgttcacgt cagcagacgg aaccacacgc agtgcaggca gacccagtac
    14761 atgttcagca atgtacggca gcaggtcgat aggcagtaaa tccatcaggt tcatcaggaa
    14821 tacccagacg aagatcgtca gggccagcgg agcaatcagc ttgcttttgc catggtacat
    14881 gtctttcacg ctaccattaa caaagccgat caccagctca atcgcggtct gaaacttacc
    14941 tggcacgccg ctggttgcct ttttggctac actacggaat aaaaccagga acaacagacc
    15001 cagcaccacc gagaagaaca tggagtcaat attgattgtc cagaaggtgg ctggggggtt
    15061 atgtggatcc accagcgaga atgtacgcag gtccagctga aggttattca ggtggtgtcc
    15121 tatgtaatcc tgcggcgtca tattttctga agccatgatg ccttttaccc tttgttgtta
    15181 attacagccg gtgccagtat ctgaaccacc agcaccaaaa cccacgtaac gatcagcggc
    15241 aagaataccg cctttaaaac cgccaacgcc accaccagta acaccaacat cgccagaact
    15301 ttgaaagctt cgccaaatgc gaatgtccag gccacccggc ctttcgctgg tgtatgcgcc
    15361 tggtgacgcc aggcaaatat cataaacaaa acgttaggca gaaagactgc caggcccccg
    15421 cttattgcag agacgcccca gaaggggtct ttgaggctga acagcaatcc acttgctatc
    15481 accaccagta actgaacgag cagaagcttc cgagcaacgt ttcgactcac gagcgacaca
    15541 gacatcacgt ttttcactcc tgctcccttc gaggtatgcc gcgtgtcgta taaaactttc
    15601 tttaaggctt agagtcaagc atcaaaaagc ggtcaaatta tacggtgcgc ccccgtgatt
    15661 tcaaacaata agtagccaaa aggtgaataa atgtttaaat atttttccag tgcatacaat
    15721 tgcgactttt ctgcaaaccc tgttcgatca tgaaaaactg taaataacgc gtaaacactg
    15781 gtgataaagc gtgcttcaga tcacatattg cgcatgttcg cgcacagcat atttatttac
    15841 ttggcaaatg atgcctttgc aagtttatga tatttcagtc taaaaacaga tactgtttta
    15901 ataaatgaca tttacataac aaaaaccacc cattgacatt tttaataatg ttttaacagt
    15961 caatgatgat tcttagcgcc gatttttagc agactgatat tttcacttat gacttatttt
    16021 ctgcttacca aaaaaagcca cgttatcttg ttgatacaaa agagtgaacg tggcgttaaa
    16081 tgtaaccagt tatatcagta gaaaacctgg ttgttgttaa cagtctaacc ggtcaatttt
    16141 ttatgatttt tttgataaaa attaaatttt atttgcttta atcaccacca gatgacgttc
    16201 gccatccagg gctggaacct gaagtttaac cactgattcg acctgatatt cttcgggcaa
    16261 caaagcgatt tcatcttccg gcatttgccc tttcagcgcg tagaaacggc cttgctcacc
    16321 aggaagatgg tggcaccagc tcaccatatc gttcagagag gcaaaagcgc ggctaattac
    16381 gccatcaaat ggcggctctg acggaaactc ttctaccctg ctttgtactg gctcaatatt
    16441 ctccagttta agctcatgtt gcacctgacg aaggaaacgc acgcgtttac caaggctatc
    16501 caacaacgtg aaatgagctt caggacgcac gatagagagt ggaatgccag gcagtcccgg
    16561 tccggtgccg acatcgataa accgttcacc ttgcagatac ggtgccacca caatgctatc
    16621 gagaatatgg cgtaccagca tctcattagg atcgcggacc gaagtcaggt tgtacgcttt
    16681 attccattta tgcagcatat tcacgtaggc aataagctgg tttttctggt gatcggtaag
    16741 cgaaatacct gcgtctttca gcagtaagga gagtttgttg agcacggtga ttacctgttc
    16801 ttgatgcgtt gcctggtaag cgggtgctta ccaggcattt ttaatgcgtt atgcgctacg
    16861 acgcagcata ccctgttttt tcagccacac cagcagaatg gagatagccg caggcgtgac
    16921 gccagaaata cgcgaagcct ggccaataga ggccggtttg tgatcgttaa gtttggcgat
    16981 cacttcgtta gaaagaccgg atacctggcg gtaatccagc gtcgctggta gcagggtatt
    17041 ctcgttacgc agctgctttt cgatctcatc ttgctggcgc gcgatataac cttcgtattt
    17101 aacctgaatc tcaacctgtt ccgcagcctg ttcatctgtc aacgcagggg caaacggtgt
    17161 cagcgtggtt aatttttcat aagtcatttc aggacgacgt agcagatctt caccactggc
    17221 ttcacgggaa agcggcgcag tcaggtgagc attcacttcg gctgcagctt ccgccgacgg
    17281 ggttacccag gtcgatttca gacgctgacg ctcacgctcg atattctcaa gtttctcgtt
    17341 aaagcgcgcc caacgttcgt catccaccag gcccagttcg cgaccgattt cagtcaaacg
    17401 cagatcagca ttatcttcgc gcagcatcag acgatattct gcgcgcgagg taaacatacg
    17461 atacggttct ttggttccta aagtgcacag gtcatcaacc agtacgccga gatatgcctg
    17521 agaacgcgcc ggagcccaac cttctttgtc agcagacaga cgggcagcgt taagaccagc
    17581 cagcaaacct tgcgcagcgg cttcttcgta accggtagtg ccgttaatct gaccagcaaa
    17641 gaacagcccc tggataaact tgctctccag cgtcggtttc aggtcgcgtg gatcgaagaa
    17701 gtcatactca atggcataac ccggacgcac gatctttgcg ttttccatcc cctgcatgga
    17761 gcggacgatt tgcatctgca catcgaacgg caggctggtg gagataccgt tcggataaat
    17821 ttcgttagag gtcagccctt ccggttcaag gaagatctga tgctgatttc tgtcggcgaa
    17881 gcgcatgact ttgtcttcga tcgacgggca gtagcgtggg ccgacacctt cgatcacccc
    17941 tgcgtacatt gggctacgat cgaggttact gcggatcaca tcatgggttt tctcgttggt
    18001 atgagtgata taacacggca cctgctgggg atgctgggac gcattgccca taaacgagaa
    18061 tactggcatt gggttatagc catgctgttg cgccagtacg ctaaagtcga tggttcgagc
    18121 atcaatacgc ggtggtgtcc cggttttcag acgaccaacg cgcagcggca gttcacgcaa
    18181 acggcgagaa agcggaatgg acggcggatc accagcacgg ccaccgctgt aattatccag
    18241 accgatatga attttaccgt cgaggaacgt cccaacggtg agcacgacag ctttggcacg
    18301 gaacttcagt cccatttggg taacggcacc gaccacgcga tcgttttcga caataagatc
    18361 ttcaactgcc tgctggaaga tcatcaggtt cggttggttc tccagcgccg tacgtaccgc
    18421 ctgacggtag agcacacgat ctgcctgagc tcgggtagcg cgaaccgccg gtcccttgct
    18481 tgcgtttagt atcctaaact ggatacccgc ctgatcgatc gctttcgcca tcagaccgcc
    18541 gagtgcatcc acttctttta ccagatgtcc cttcccaata ccgccgatcg ccgggttgca
    18601 gctcatctgc cccagagtgt cgatattgtg tgtcaaaagc agagtctgtt ggcccatacg
    18661 cgccgcggcc atcgcggcct cggtgcctgc atgacccccg ccaatgatga tgacgtcaaa
    18721 aggatccgga taaaacatgg tgattgcctc gcataaagcg gtatgaaaat ggattgaagc
    18781 ccgggccgtg gattctactc aactttgtcg gcttgagaaa gacctgggat cctgggtatt
    18841 aaaaagaaga tctatttatt tagagatctg ttctattgtg atctcttatt aggatcgcac
    18901 tgccctgtgg ataacaagta tccggctttt aagatcaaca acctggaaag gatcattaac
    18961 tgtgaatgat cggtgatcct ggaccgtata agctgggatc agaatgaggg gttatacaca
    19021 actcaaaaac tgaacaaccg ttgttctttg gataactacc ggttgatcca agcttcctga
    19081 cagagttatc cacagtagat cgcacgatct gtatacttat ttgagtaaat taatccacga
    19141 tcccagccat tcttctgccg gatcctccgg aatgtcgtga tcaagaatgt tgatcttcag
    19201 tgtttcgcct gtctgttttg ctccggaatt tttgagttct gcctcgattt tatcgatagc
    19261 cccacaaaag gtgtcatatt cacgactacc aataccgatt gcgccaaagc ggaccgcaga
    19321 aagatcgggc ttctgttcct gcaatgcttc atagaaagga gaaaggttgt ccggaatatc
    19381 tccggcaccg tgggttgagc tgataaccag ccagatcccc gaggcagata aatcttccaa
    19441 cagcggaccg tgcagcgttt cggtggttaa acccgtctct tccagctttt cagccaggtg
    19501 ttctgctaca tattcggcac cgccgagggt gctgccgctg ataagagtga tatctgccat
    19561 aaaccgccac ctttattaag agtggcgtat tgtacgctgt gaacgcgttg ggatctacct
    19621 gtggaaaagt atgggattaa aaaagccgat cagggcttaa tggtacgcat gatcgggttc
    19681 tgcaggacga tcagtgtctc ggtggactga atttcatcaa ttgtttggat cttgttgata
    19741 agtacatgct ggagagcatc gatcgaacgg cacatcactt ttataaagat gctgtagtgg
    19801 ccggttgtgt aataggcttc agtgacttca tcgaggcttt ccagctttgc cagcgcggaa
    19861 ggatagtctt tggcgctctt taatataatg ccgataaagc agcctacgtc ataaccaagc
    19921 tgcttcgggc tgacatcaat acgcgccccg gtaatgatcc ccgcctgctt cattttctct
    19981 actcgaacgt gaatcgtccc cggactgacg ccaaattgtt tcgccagttc ggcgtaagcg
    20041 gtgcgcgcat tgcccattaa tgcttccagg atgccacggt ccagattgtc gatcagataa
    20101 ttttccatag gattttctta tgcggattga tgattcattc tattttagac ttctttttta
    20161 atgaatcaaa agtacgtgag gctttttatt gaatgattat tgcatgtgtg ttggtttttg
    20221 ttgcttaatc ataagcaaca ggacgcagga gtataaaaaa tgaaaaccgc ttacattgcc
    20281 aaacaacgtc aaattagctt cgtgaaatct cacttttctc gtcaactgga agaacgtctg
    20341 gggctgatcg aagtccaggc gccgattctt agccgtgtgg gggatggcac gcaggataac
    20401 ttgtcgggct gtgaaaaagc ggtgcaggta aaagtgaaag ctctgcctga tgcccagttc
    20461 gaagtggttc attcactggc gaagtggaaa cgtcagacct tagggcaaca cgacttcagc
    20521 gcgggcgaag ggctgtacac gcacatgaaa gcccttcgcc ccgatgaaga ccgtctttcc
    20581 ccgttgcact cggtctatgt agaccagtgg gactgggagc gcgtaatggg cgacggcgag
    20641 cgtcaattct cgactctgaa aagcacggta gaggcgatct gggcgggaat taaagcaacc
    20701 gaagctgcgg ttagcgaaga gtttggcctg gcaccgttcc tgccggatca gatccatttc
    20761 gtacacagcc aggagttact gtctcgttat ccggatctcg atgccaaagg gcgtgagcgg
    20821 gcgattgcga aagatcttgg cgcggtattc ctcgtcggga ttggcggcaa gttgagtgat
    20881 ggtcatcgcc acgacgtgcg cgcaccggat tatgatgact ggagcacccc gtcagagctg
    20941 ggccatgcgg gtctgaacgg cgatattctg gtgtggaacc cggtactgga agatgcgttt
    21001 gagctttctt ccatggggat ccgcgtggat gccgacacgc tgaagcatca gctggcgctg
    21061 accggtgacg aagatcgcct ggagctggag tggcatcagg cgctgctgcg cggtgaaatg
    21121 ccgcagacca tcggcggcgg tatcgggcag tctcgtttga ccatgctgct gctgcaactg
    21181 ccacatatcg gccaggttca gtgtggtgta tggccggcag ctgttcgcga gagcgtccct
    21241 tctctgctgt aataatttat cgccgccagc gtctgagcag gcggcttcgc atcccggtat
    21301 caaagcgcca gatatgatcg aaaatgcgca tgatgccggg tttgccgtgt gccgacatcg
    21361 ccacggcatg aaagcgatgc tgatgtaccc gctgcaactc tttcacttta ctcgtcacgt
    21421 cgtcaggcaa ccgctgggcg ataaaatcag aaatcaccac cgcatcggca tcaaaccatt
    21481 ccctgctttg caagcgttcc ataatggcgc gaaaacaact ggcaagatcg gtgccgccac
    21541 gaaactgctg acttaaaaag cggatcgctt gctcaatacc ttgtggccct gaaagctcat
    21601 aacggacgat ctcggtggaa aatagcataa tatagcagcg ccggttttca gcgagggcta
    21661 tgcgcatcaa ggccaggcag aacgctttcg cacactgttc attaaagccg cccattgagc
    21721 cggaagtatc cacgcagaca ataaacggtc cgcgcggctg ttcgtcgtaa tctttatgca
    21781 ccaccgggcg ttcgatcatt ttttcacgcc acgactcacc gtgcaggcga taggtaagca
    21841 actgtttttc caccagccga cggtaaaact catactccag ttccgttatc cctagtgtcg
    21901 ccagttctgg cggcaagaga cgtaaaatat catcgctttg ctgcagacca tctacctgct
    21961 caggaactgt cgccggttcg cgcaccatgg tgcggaaggt ttccatctgc gcatcgttgc
    22021 gcggtattga tttggcttcc cgggaacgcc ccagctgttc tgccagacgt ttcagttccg
    22081 gctgttcgtt aagaaattca ccgtatttca caatcaactg atagtcgcca cgtttaagct
    22141 gaccggcact catatcccac agacgaccag ctgcggtatt gttatctgcg agaatcggtt
    22201 caagttgccc gctcagcgtc atgcgttcct gaacttcact caacagttgt tcgcgttctt
    22261 cttctaatag ctgctgattt aacgtcgttg cttgcacgat caggcttaaa cgccagcgct
    22321 ggagaaaaag cgtgtgtaac gctgaagtga tcgtactgtt agcatcaacc aactgtcggg
    22381 cttgttctgc ccatggggaa ttcagacgat gcagtaagtc caggatttgt ggtagctgca
    22441 caataaactg cggcgtggag aggagctggc tttgctgata gcacatcacc tcttcggtga
    22501 gttccggcgg gactcgggca tctttcagcc gactgcgcag cgcctcacgc cagcggggaa
    22561 catcatcagt gattgccgcc ttcagtcgtg ggaatttttc aaagaaaaca gccagctgcg
    22621 gtgaggccag cagcgcgatg atcatctctt cgatcaatcc ctcttcgctg acggccagca
    22681 tcacattaag cgtatccagc gttagcattg ttgtgcctgg cgaatctgtg cgccgacatc
    22741 ctgtaggctg gcttcaatgc gacctaacca gtcgccaggg ataaacagac atttttgctg
    22801 ttcgctgaat aaagcgtgtt gcttgcgcca gtcgctttca agctcttcca gttgttgttt
    22861 tatttcgctg ggcagacctt cagccgatga accgggaagt gctagcgtac tgccttgtaa
    22921 actcacatcg cgtacaacca gatgttgggc gctatcgact tccagattca gtttctgggc
    22981 aaagccgata ccgttcagtt tgccgcgaat ctcaccacct ttactcagcc actgttccag
    23041 cgcgctacgc tcaaaggaga tatgaaccac ttccatatca tgcagtttta acggtttttg
    23101 cagcagcaga gtcagagtgg aagcagtaac gttaacaggg agttgatact gctgacgacg
    23161 gctaaaaatg ccgcccagac gaattaccgt taaggctgtt ttatcgcttt gttgctgctg
    23221 aagttgcagg tgacgttgca caatcgcgcc catgcgggtc aacatccctt gctgttgcca
    23281 ggcgtgaccg gtcatcaata catcaatttg ttgttgtatc aaattcaggc tttgcgcgtc
    23341 ataccacagg caatctttca gcaaaatgag atcaaccggg gcgacagcac tgcgaccgct
    23401 aaaaaaggcg ctggcctgca ataagcggat cgcttttttc cagcgacgat ccgagacata
    23461 aggcgcatcc ggtaatttat ccagttgctg gcgcagcata aaaatcagct caaatacatg
    23521 atcgggcagc gtaatttcac caatctcttt ctgccagcgt tcatattctt catctgtgat
    23581 ctgcaaggag gcaggaaccg gattgtcatt ttcatcctgt tgactggtca gcatggagcg
    23641 gaaattcgct ttatcctgca ctttatctaa ccacagacga atcagcatgc ggtcatataa
    23701 cgcttccaga ctgctgtctg cttccggcag ctcgttggag gccgccacca gcagacgcat
    23761 cgggattttt tctacgagtg cgccgttgcg gaactggcgc tcgttaatgg cggtgagcag
    23821 ggtattaaga attgccggac ccgctttcca gatctcatcc agaaagacga tttcagcttc
    23881 cggcaggtaa ccgctggtta aacgttcata gcgcccttca tcttttagcg cctgaataga
    23941 aaggggacca aaaacttctt ccggcgtgga gaagcgggtc atcagatatt caaacgcgcg
    24001 ggcattctga aaggcgaatt ttaagcgccg ggcgatcaaa cttttggcaa tacctggtgg
    24061 gccaaggagg aacacacttt caccacttaa tgccgctaat aaacacaagc ggatggcgtg
    24121 gctacgttca taaagcccct tttccaacga actgctcagg cgggaaattc tttccgctaa
    24181 taaatgaggg tgagccataa tgaagtggcg tcctttcgtc aaaagttctg cgtaaattgc
    24241 gagtatagac gtttcttgct ggtggctaaa atagtctcaa aggaggggta tttttctttg
    24301 agccaggtta atgtggccgc atttaggagt acgattttgc cgttaatcgt gcatactgtg
    24361 cgcttttttg tgggccaagg gactaagcac acatttcata tttcaacgaa agactagtct
    24421 atgagcactg ataataagca atcattgccc gcgattaccc tcgcggcgat tggagttgtc
    24481 tacggcgata ttggtaccag cccgttatat acacttcgtg aatgtttgtc cggccagttt
    24541 ggttttggcg ttgaacgcga tgccgtgttt ggctttttat cgctgatctt ctggctgcta
    24601 atctttgtgg tttccattaa atatctcacc ttcgtgatgc gggcagataa cgccggtgaa
    24661 ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata cgtcggcgcg aaccacatca
    24721 atgctggtga ttatggggct aatcggcggc agctttttct atggtgaagt cgtcattact
    24781 cccgctattt cggtgatgtc cgccattgaa ggtctggaaa tcgtcgcccc gcagctggat
    24841 acctggatag ttcccctctc aattatcgtt ctcacattat tattcatgat tcaaaaacat
    24901 ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc tgacctggtt tttgattctg
    24961 gcagggctgg ggttacgtag cattattgct aacccggaag tgctgcatgc actgaatccg
    25021 atgtgggcga tgcatttctt ccttgaatac aaaacggttt cttttattgc attaggggca
    25081 gtggtgctgt cgattacggg gggcgaggcg ctgtatgctg atatggggca ctttggtaag
    25141 ttccctattc gtctggcgtg gttcaccgtc gtattgcctt ccttaaccct taattacttc
    25201 ggccagggag cgctgttgtt aaagaatccg gaagcgatta agaacccgtt cttcctgttg
    25261 gcaccggact gggcgctgat cccgctgctg atcatcgccg cactggcgac ggtaattgcc
    25321 tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc aggcggtacg tctgggatat
    25381 ttgtcgccga tgcgcattat tcacacttcc gaaatggagt cagggcaaat ctatattccc
    25441 tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga ttgtcagctt tgagcactcc
    25501 agcaacctgg cggcggcgta cgggattgcg gtgaccggaa ccatggtgct gacgtctatt
    25561 ctctcgacta ccgtggcacg tcagaactgg cactggaata agtattttgt tgcgctgatc
    25621 ctgattgctt tcctttgtgt cgatattccc ttgttcaccg ctaacctcga taaactgctc
    25681 tccggcggct ggttgccatt gagcctcggt acggtgatgt ttatcgtgat gaccacctgg
    25741 aagagcgagc gtttccgctt gctgcggcgg atgcatgaac atggtaactc tctggaagcg
    25801 atgattgctt cgctggagaa atcaccgccc gttcgcgtgc ctgggaccgc ggtgtatatg
    25861 tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata acctgaaaca taacaaggta
    25921 ttgcatgagc gggtgattct gttaactttg cgcaccgaag acgcgccata tgtccataac
    25981 gtccgtcggg tacagattga acaactgtcg cccactttct ggcgcgtggt ggcaagttat
    26041 ggttggcgag aaacgccaaa cgtggaagaa gttttccacc gctgcggtct ggaaggatta
    26101 agttgccgga tgatggaaac ctccttcttt atgtcgcatg agtcgttgat cctcggcaaa
    26161 cgaccgtggt atttgcgtct gcgcggcaag ctgtacttgc tgctgcaacg taatgcgctg
    26221 cgggcaccgg atcaatttga aatcccgcca aacagagtta tcgaactggg tactcaggtc
    26281 gaaatctaac gccagacgcc tcctttctcc ataagggggc gtttttgttt tcatggttaa
    26341 tcaccatgta aaacgtttcg aggttgatca catttccgta acgtcacgat ggttttccca
    26401 actcagtcag gattaaactg cgggtcagcg aaacgtttcg ctgatggaga aaaaaatgaa
    26461 aaaaggcacc gttcttaatt ctgatatttc atcggtgatc tccctactgg gacataccga
    26521 tacgctggtg gtgtgtgatg ctggtttacc catccccaaa agtacaacgc gtatcgatat
    26581 ggcattaacc cagggtgtac cttcttttat gcaggtgctg ggtgtagtca caaatgaaat
    26641 gcaggtcgag gctgtcatta tcgcggaaga gatcaaacaa cataatccgc aactccacga
    26701 aacgttgctc actcaccttg agcagctgca aaaacaccag ggaaacacca ttgaaattcg
    26761 ttacaccacg catgaacaat tcaaacaaca aaccgcagaa agtcaggcgg taattcgcag
    26821 cggagaatgt tctccgtatg cgaatatcat tctctgtgct ggcgtgacgt tctgaggccg
    26881 tcatggaagc attacttcag cttaaaggca tcgataaagc cttcccgggc gtaaaagccc
    26941 tctcgggcgc agcgttaaat gtctatccgg gccgcgtgat ggcgctggtg ggcgaaaacg
    27001 gcgcgggtaa atccaccatg atgaaagtgc ttactggtat ctatactcgc gatgccggta
    27061 cgcttttatg gttggggaaa gaaacgacat ttaccgggcc gaaatcttcc caggaagccg
    27121 ggattgggat tatccaccag gaactgaacc tgatcccgca gttaaccatt gccgaaaaca
    27181 ttttcctcgg tcgtgagttt gttaatcgct ttggcaaaat tgactggaaa accatgtatg
    27241 ccgaagcgga taaattgctg gctaaactta acctgcgctt taaaagcgac aagctggtgg
    27301 gcgatctttc catcggtgac cagcaaatgg ttgaaatcgc caaagtgctg agttttgagt
    27361 cgaaagtcat cattatggat gaaccgaccg atgcgctgac cgataccgaa accgaatcct
    27421 tgttccgcgt catccgcgag ctgaaatcgc aaggccgtgg tattgtctat atctcccacc
    27481 gcatgaaaga aatcttcgag atttgcgatg acgttaccgt ttttcgtgac ggacaattta
    27541 ttgctgagcg cgaagtggca tcactgaccg aagattctct gattgagatg atggtgggcc
    27601 gcaagctgga agatcaatat ccgcacctgg acaaagcgcc gggagatatc cgcctgaaag
    27661 tcgataatct ctgcggacct ggcgttaacg atgtctcttt tactttacgc aaaggcgaaa
    27721 ttcttggcgt ctctggtttg atgggcgctg ggcgtaccga actgatgaaa gtgctctacg
    27781 gtgcactgcc gcgcacaagc ggttacgtca ccctggatgg gcatgaagtc gttacccgtt
    27841 cgccgcagga tggcctggca aacggcattg tttatatctc cgaagaccgt aaacgtgacg
    27901 gtttagtgct gggcatgtca gtaaaagaga acatgtcgct gaccgcgctg cgctacttca
    27961 gccgtgctgg cggcagtttg aagcatgccg atgaacagca ggctgtgagt gatttcattc
    28021 gtctgtttaa tgtgaaaacg ccatcgatgg aacaggcaat tggtctgctt tccggtggca
    28081 atcagcaaaa agtagcgatt gcccgtggtc tgatgacacg ccctaaagtg ttgatcctcg
    28141 atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa ctgattaacc
    28201 agttcaaagc cgatggcttg agcatcattc tggtgtcatc ggagatgcca gaagtattag
    28261 gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa tttactcgtg
    28321 agcaggccac ccaggaagtg ttaatggctg ccgctgtggg caagcttaat cgcgtgaatc
    28381 aggagtaaaa aaatgacaac ccagactgtc tctggtcgcc gttatttcac gaaagcgtgg
    28441 ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt ctcgacgtta
    28501 agcccgaact ttttcaccat caataactta ttcaatattc ttcagcaaac ctcggtgaac
    28561 gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggtatcga cttgtcggta
    28621 ggttctctgt tggcgctgac cggcgcagtt gctgcatcta tcgtcggcat tgaagtcaat
    28681 gcgctggtgg ctgtcgctgc cgctctcgcg ttaggtgccg caattggtgc tgtaactggg
    28741 gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat gatgctttta
    28801 ctgcgcggcg tgaccatggt ttataccaat ggtagcccag tgaataccgg ctttactgag
    28861 aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc gacgccagtc
    28921 tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac gcgtctgggg
    28981 cgttacatct acgcgctggg cggcaatgaa gcggcaacgc gtctttctgg tatcaacgtc
    29041 aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct ggccgggatc
    29101 attgaagtgg cgcgtctctc ctccgcacaa cccacggcgg ggactggcta tgagctggat
    29161 gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg tcgcattgtt
    29221 gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa tttgttaggt
    29281 gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc ggtgctggta
    29341 gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat gaaaaaactg
    29401 gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc gatggcaaaa
    29461 gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc gctgaaagat
    29521 ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga ctcccagaac
    29581 aacccggcga aagagctggc gaacgtgcag gacttaaccg tccgcggcac caaaattctg
    29641 ctgattaacc cgaccgattc cgacgcagta ggtaatgctg tgaagatggc taaccaggcg
    29701 aacatcccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt gagccacatt
    29761 gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa gaaagcgggt
    29821 gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc ccgtgaacgt
    29881 ggcgaaggtt tccagcaggc cgttgctgct cacaagttta atgttcttgc cagccagcct
    29941 gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac cgctcatccg
    30001 gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc ttggcgcgct gcgcgcactg
    30061 caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc ggatggcgaa
    30121 aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga tcagattggc
    30181 gcgaaaggcg tcgaaaccgc agataaagtg ctgaaaggcg agaaagttca ggctaagtat
    30241 ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat gacctgatgg
    30301 tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag ggcacgcgcc
    30361 accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca ggcagcctcg
    30421 ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt cctactccag
    30481 gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc gcgaatcagg
    30541 cggtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgcact ggcgatgaca
    30601 gcattggtga gagcgttcgc cagcagctcg ccactgataa catcgatatt tctccggtca
    30661 gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc gaaggtgaga
    30721 atgtcatcgg tattcatgcc ggcgctaatg ctgccctttc cccggcgctg gtggaagcgc
    30781 aacgtgagcg tattgccaac gcgtcggcat tattaatgca gctggaatca ccactcgaaa
    30841 gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg cttaacccgg
    30901 ctccggctcg cgaacttcct gacgaacttc tggcgctggt ggacattatt acgccaaacg
    30961 aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat gcagcgaagg
    31021 cggcgcaggt actgcatgaa aaaggtatcc gtactgtact gattacttta ggaagtcgtg
    31081 gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg gtgcaggctg
    31141 tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg ttgctggaag
    31201 aaaaaacatt gccagaggcg attcgttttg cccatgcagc cgctgcgatt gccgtaacac
    31261 gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt ttagacaggc
    31321 agaggtgacg cttggctaca atgaaagatg ttgcccgcct ggcgggcgtt tctacctcaa
    31381 cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattaca gccaaagttg
    31441 aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc ctcaaactca
    31501 atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc tattcagaac
    31561 tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtcttgtc ctttgcaata
    31621 ccgaaggcga tgaacagcgg atgaatcgta atctggaaac gctgatgcaa aaacgcgttg
    31681 atggcttgct gttactgtgc accgaaacgc atcagccttc gcgtgaaatc atgcaacgtt
    31741 atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac agcgatctta
    31801 ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc gataaaggtc
    31861 atacccgtat tgcctgtatt accggtccgc tggataaaac tccggcgcgc ctgcggttgg
    31921 aaggttatcg tgctgcgatg aaacgtgcgg gtctcaacat tcctgatggc tatgaagtca
    31981 ctggtgattt tgaatttaac ggcgggtttg acgctatgcg ccaactgtta tcacatccgc
    32041 tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt taccaggcgt
    32101 tatatcaggc agagttacag gttccgcagg atatcgcggt gattggatat gacgatatcg
    32161 aactggcaag ctttatgacg ccaccattaa caactataca ccaaccgaaa gatgaattgg
    32221 gggagctggc gattgatgta ctcatccatc ggataaccca gccgacccta cagcaacaac
    32281 gattacaact tactccgatt ctgatggaac gcggttcggc ttagatttac gctgtctttt
    32341 gatcaaatta ttaccatcgg ttgttttcag tagcatgaac attgctgctg aagcaacagt
    32401 aataatgccc atcgtgataa acgtatagtg gaattgttcg actgtcgttg tgccttccat
    32461 cccttcgtaa acgcgaagga cggccgcact tacagcaacg cctaaactga tagacagttg
    32521 ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca gatcggcaag
    32581 tgtgatggta ttcatcgcgg taaattgcgt cgacatagcc atccctaata taaacaacgg
    32641 caagatcagc atccatatcg ccattgctgg tgattgcaaa gagaactgag cgatcattag
    32701 cccaataatc accgtgatcc ccactaacgt atggcgatag cccagacgac gtaagacttg
    32761 ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca tacagccagc
    32821 aataaacgcc tgatacccaa atcctacctg taacatcaat ggcataagga acggtacaca
    32881 gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag tgcgggtttt
    32941 aaataaatct aatgaaatta atgggtttgg cgtgcgtcgc gcatggagaa tatagagaag
    33001 cagtaaccag atgctggtga caattaccgt caaggcaatc cagctggcga caatcttttc
    33061 cccgaatagc tctattccgc ttgagaagag aacaaggctg aggccaaaca gcaaaaagcc
    33121 agtggtatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc gcgcgtaaag
    33181 aaggcccgca atacctatgg ggatattgat taaaaatatc cagtgccagg ttgcccaggt
    33241 gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg gcatggcgac
    33301 aaaattcaat acaggaagaa gttcattacg aggataagcg cgcagtaagg ccagccgagc
    33361 aacaggcatc atcattgcgc cgcctatccc ctgaataacc cggaagacaa ccagctgtgg
    33421 tagcgagttg gaaagtgcgc aggccagaga acccaacgtg aacagactca cggcaagggt
    33481 aaaaatgcga cgtgtaccga agcgatcggc tagccatccg cttaccggaa taagcatcgc
    33541 caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac gattaaggct
    33601 atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct gcatgaagaa
    33661 ggccatcgcc gcgatccacg gcaaacccgc catactgcgc ttctttttat cgctcattca
    33721 atgtcctgtt atcgggttat cacttatctg gtgagcgtag cagcgcctga caagctttaa
    33781 atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc agatccagct
    33841 ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa tggaatagcg
    33901 aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca tgccaggcca
    33961 tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc atttcggcca
    34021 ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac ctttgctgcc agtaggcaag
    34081 cttgcggctc caaacagata cgtataacca gaaagtgatc gatgacctga tgaaagttct
    34141 cttctgtcat ccaccaggta agcaattcct gatcaagaaa attccagttc gattgtggca
    34201 tgactcgggt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt aacgttttga
    34261 tcgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca atctcaccag
    34321 gcaaaatggt gccgggttca tattcacctt ttaagatccg ctgcgccagc ttctcagcca
    34381 gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc attgctaatc
    34441 ttcctttttc tttttattcc ttcttagtat gccaccagga agtgtgatta cggttgcaaa
    34501 aacggcaaat tgattgtttt atggcacatt aacggggctt ttgctgaaaa aatgcgcggt
    34561 c
//
LOCUS       DAZGJT010000043        33883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZGJT010000043 DAZGJT010000000
VERSION     DAZGJT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.043"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            325..777
                     /locus_tag="ACHKFK_002284"
     CDS             325..777
                     /locus_tag="ACHKFK_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9052620.1"
                     /translation="MIVGNIHHLQSWLPEELREAIEYIKSHVSDETAKGKHAIDGDRL
                     FYLISEDTTEPGELHRAEYHARYLDIQIVLKGQEGMTFSTQPAGVPETDWLADKDIAF
                     IGQGIDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKLLKS"
     gene            complement(822..1826)
                     /gene="argF"
                     /locus_tag="ACHKFK_002285"
     CDS             complement(822..1826)
                     /gene="argF"
                     /locus_tag="ACHKFK_002285"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO9052621.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEARLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVAECSALAQKHGGKITLTED
                     IASGVKGADFIYTDVWVSMGEPKEKWAERIALLRDYQVNSKMMALTGNSQVKFLHCLP
                     AFHDEQTTLGKKMAAEFGLYGGMEVTDEVFESPASIVFDQAENRMHTIKAVMVATLAK
                     "
     gene            1988..2404
                     /gene="rraB"
                     /locus_tag="ACHKFK_002286"
     CDS             1988..2404
                     /gene="rraB"
                     /locus_tag="ACHKFK_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO9052622.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGIRH"
     gene            complement(2581..3084)
                     /gene="yjgM"
                     /locus_tag="ACHKFK_002287"
     CDS             complement(2581..3084)
                     /gene="yjgM"
                     /locus_tag="ACHKFK_002287"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9052623.1"
                     /translation="MNVVASPALRLRKLTVADNPAIAHVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLRKL"
     gene            3277..4450
                     /locus_tag="ACHKFK_002288"
                     /pseudo
     CDS             3277..4450
                     /locus_tag="ACHKFK_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
     gene            complement(4497..7352)
                     /gene="valS"
                     /locus_tag="ACHKFK_002289"
     CDS             complement(4497..7352)
                     /gene="valS"
                     /locus_tag="ACHKFK_002289"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO9052624.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIVDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(7352..7795)
                     /gene="holC"
                     /locus_tag="ACHKFK_002290"
     CDS             complement(7352..7795)
                     /gene="holC"
                     /locus_tag="ACHKFK_002290"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO9052625.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSNPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(8053..9564)
                     /gene="pepA"
                     /locus_tag="ACHKFK_002291"
     CDS             complement(8053..9564)
                     /gene="pepA"
                     /locus_tag="ACHKFK_002291"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO9052626.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            9718..9768
                     /gene="ytgA"
                     /locus_tag="ACHKFK_002292"
     CDS             9718..9768
                     /gene="ytgA"
                     /locus_tag="ACHKFK_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO9052627.1"
                     /translation="MSIIILATISTTQENK"
     gene            9831..10931
                     /gene="lptF"
                     /locus_tag="ACHKFK_002293"
     CDS             9831..10931
                     /gene="lptF"
                     /locus_tag="ACHKFK_002293"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9052628.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10931..12013
                     /gene="lptG"
                     /locus_tag="ACHKFK_002294"
     CDS             10931..12013
                     /gene="lptG"
                     /locus_tag="ACHKFK_002294"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9052629.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(12174..13676)
                     /gene="yjgR"
                     /locus_tag="ACHKFK_002295"
     CDS             complement(12174..13676)
                     /gene="yjgR"
                     /locus_tag="ACHKFK_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO9052630.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAEEGTSSEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASIEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(13754..14752)
                     /gene="idnR"
                     /locus_tag="ACHKFK_002296"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(13754..14752)
                     /gene="idnR"
                     /locus_tag="ACHKFK_002296"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFO9052631.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(14819..16138)
                     /gene="idnT"
                     /locus_tag="ACHKFK_002297"
     CDS             complement(14819..16138)
                     /gene="idnT"
                     /locus_tag="ACHKFK_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFO9052632.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(16203..16967)
                     /gene="idnO"
                     /locus_tag="ACHKFK_002298"
     CDS             complement(16203..16967)
                     /gene="idnO"
                     /locus_tag="ACHKFK_002298"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFO9052633.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(16991..18022)
                     /gene="idnD"
                     /locus_tag="ACHKFK_002299"
     CDS             complement(16991..18022)
                     /gene="idnD"
                     /locus_tag="ACHKFK_002299"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFO9052634.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSKLHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            18239..18802
                     /gene="idnK"
                     /locus_tag="ACHKFK_002300"
     CDS             18239..18802
                     /gene="idnK"
                     /locus_tag="ACHKFK_002300"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO9052635.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQDDEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(18806..19825)
                     /gene="ahr"
                     /locus_tag="ACHKFK_002301"
     CDS             complement(18806..19825)
                     /gene="ahr"
                     /locus_tag="ACHKFK_002301"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO9052636.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLIAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLPVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            20021..20105
                     /locus_tag="ACHKFK_002302"
     tRNA            20021..20105
                     /locus_tag="ACHKFK_002302"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20055..20057,aa:Leu,seq:caa)
     gene            20292..21557
                     /locus_tag="ACHKFK_002303"
     CDS             20292..21557
                     /locus_tag="ACHKFK_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9052637.1"
                     /translation="MALTDIKVRAAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARVRRDEARNLLANGVDPGDKKKNDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEV
                     AARLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASCNRQHRPALELKRIPELLTKIDS
                     YTGRPLTRWAIELTLLIFIRSSELRFARWSEIDFEASIWTIPPEREPIPGVKHSHRGS
                     KMRTTHLVPLSTQALAILKQIKQFYGAHDLIFIGDHDSHKPMSENTVNSALRVMGYDT
                     KVEVCGHGFRTMACSSLVESGLWSRDAVERQMSHMERNSVRAAYIHKAEHLEERRLML
                     QWWADFLDVNRERFISPFEYAKINNPLKQ"
     gene            complement(21874..22397)
                     /locus_tag="ACHKFK_002304"
                     /pseudo
     CDS             complement(21874..22397)
                     /locus_tag="ACHKFK_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            22984..23213
                     /locus_tag="ACHKFK_002305"
                     /pseudo
     CDS             22984..23213
                     /locus_tag="ACHKFK_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            24244..25461
                     /gene="nanX"
                     /locus_tag="ACHKFK_002306"
     CDS             24244..25461
                     /gene="nanX"
                     /locus_tag="ACHKFK_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052638.1"
                     /translation="MATAWYKQVNPPQRKALFSAWLGYVFDGFDFMMIFYILHIIKAD
                     LGITDIQATLIGTVAFIARPIGGGFFGAMADKYGRKPMMMWAIIIYSVGTGLSGIATN
                     LYMLAVCRFIVGLGMSGEYACASTYAVESWPKNLQSKASAFLVSGFSVGNIIAAQIIP
                     QFAEVYGWRNSFFIGLLPVLLVLWIRKSAPESQEWIEDKYKDKSTFLSVFRKPHLSIS
                     MIVFLVCFCLFGANWPINGLLPSYLADNGVNTVVISTLMTIAGLGTLTGTIFFGFVGD
                     KIGVKKAFVVGLITSFVFLCPLFFISVKNSSLIGLCLFGLMFTNLGIAGLVPKFIYDY
                     FPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLILLVGF
                     DIPGKIYKLSVAK"
     gene            25473..26591
                     /gene="nanY"
                     /locus_tag="ACHKFK_002307"
                     /gene_synonym="yjhC"
     CDS             25473..26591
                     /gene="nanY"
                     /locus_tag="ACHKFK_002307"
                     /gene_synonym="yjhC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,7-anhydro-N-acetylneuraminate hydratase"
                     /protein_id="HFO9052639.1"
                     /translation="MINYGVVGVGYFGAELARFMNMHDNAKITCVYDPENGENIAREL
                     QCINMSSLDALVSSKLVDCVIVATPNYLHKEPVIKAAKNKKHVFCEKPIALSYEDCVD
                     MVKACKEAGVTFMAGHIMNFFNGVQYARKLIKEGVIGEILSCHTKRNGWENKQERLSW
                     KKMKEQSGGHLYHHIHELDCVQHLLGEIPETVTMIGGNLAHSGPGFGNEDDMLFMTLE
                     FPSGKLATLEWGSAFNWPEHYVIINGTKGSIKIDMQETAGSLRIGGQTKHFLVHETQE
                     EDDDRRKGNMTSEMDGAIAYGHPGKKTPLWLASLIRKETLFLHNILCGAKPEEDYIDL
                     LNGEAAMSAIATADAATLSRSQDRKVKISEIIKHTSVM"
     gene            26634..26759
                     /gene="ythA"
                     /locus_tag="ACHKFK_002308"
     CDS             26634..26759
                     /gene="ythA"
                     /locus_tag="ACHKFK_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YthA"
                     /protein_id="HFO9052640.1"
                     /translation="MIKNFIFDNLIILAVPFMIKTSLKTNLIFFFLCVFVPRMAS"
     gene            complement(26858..27067)
                     /locus_tag="ACHKFK_002309"
     CDS             complement(26858..27067)
                     /locus_tag="ACHKFK_002309"
                     /inference="COORDINATES: protein motif:HMM:NF028057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFO9052641.1"
                     /translation="MVSSSASNVVNCETKQRTQFECIYFSQYWAKGDVIANRAPIGQW
                     EPYSEEPLLGIIVTSVCRINRDAKT"
     gene            27090..27293
                     /locus_tag="ACHKFK_002310"
     CDS             27090..27293
                     /locus_tag="ACHKFK_002310"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052642.1"
                     /translation="MDITPKYVGIASGLMNAGSAVADIISPIAFGIIIDKTGNWSLPF
                     YGSVALLVIGIFLTFFMRPDKSL"
     gene            27381..27619
                     /locus_tag="ACHKFK_002311"
                     /pseudo
     CDS             27381..27619
                     /locus_tag="ACHKFK_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            27860..28603
                     /locus_tag="ACHKFK_002312"
     CDS             27860..28603
                     /locus_tag="ACHKFK_002312"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9052643.1"
                     /translation="MKKSSRMSIVHSHAAGVDIGAKFHVVAVPPDADAEPIRTFQSFT
                     GDLHRMSDWLKTCHITTVAMESTGVYWLPAFEILEAAGFNMILVNVSAAKNVPGRKTD
                     VNDAQWLQRRHAFGLLRASFRPVHDISVLRSYLRQRERLTEYRAAHIQHMQKALMQMN
                     VQLHHAVSDITGVTGLSIVRAIVSGEPDPSVLIQYRDVRCKKTPEVLQQALTGNWQPE
                     HLFALEQSVAFFYFYQEKIRERDDQIEIH"
     gene            28758..28997
                     /locus_tag="ACHKFK_002313"
     CDS             28758..28997
                     /locus_tag="ACHKFK_002313"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052644.1"
                     /translation="MALKFVAERGTDMNRWPDASHFTSWLFCPREIRSPVEKCCPRKR
                     DVHPVELLLHSGWQPQRLVGAILRWVLFIVDYHPV"
     gene            29205..30073
                     /locus_tag="ACHKFK_002314"
                     /pseudo
     CDS             29205..30073
                     /locus_tag="ACHKFK_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(30302..31315)
                     /locus_tag="ACHKFK_002315"
     CDS             complement(30302..31315)
                     /locus_tag="ACHKFK_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO9052645.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(31327..32505)
                     /gene="fucP"
                     /locus_tag="ACHKFK_002316"
                     /pseudo
     CDS             complement(31327..32505)
                     /gene="fucP"
                     /locus_tag="ACHKFK_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
     gene            complement(32637..33617)
                     /locus_tag="ACHKFK_002317"
     CDS             complement(32637..33617)
                     /locus_tag="ACHKFK_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HFO9052646.1"
                     /translation="MSHQLTFADSEFSSKRRQTRKEIFLSRMEQILPWQNMVEVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVRTLKQHPRKSKTAINIEYMKASIRAKVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRADQMIRQWERSH"
     gene            33686..33883
                     /locus_tag="ACHKFK_002318"
                     /pseudo
     CDS             33686..33883
                     /locus_tag="ACHKFK_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT         8658 a         8135 c         8239 g         8851 t
ORIGIN      
        1 taaggtgatt atttttcaca gcactatttt cacgggataa ctatgacagc acgggcatta
       61 aaagctaaga aaatgaactg tgatctgact gagagttttc ttttaacata tgcaataaca
      121 agatattagg accaaaatac atagctaagt tataagattt atgcagcaaa actgcataca
      181 aaattactcc tgcttaacat cagtacaaca tctccagcaa taaacatgcg caagggcaaa
      241 acctgatgat ttttctatgg tgagaggtgg ctttttttcg tataaggaaa gaagtgaaac
      301 attgttttaa taaaggaaga tattatgatt gttggcaaca tccatcactt acaatcctgg
      361 ctgccggaag aacttcgcga agcaattgag tacatcaaat cacatgtcag cgatgagaca
      421 gcaaaaggta agcacgctat tgacggcgac cgcctgtttt atctgatttc tgaggatacg
      481 actgaaccag gtgaactgca tcgtgctgag tatcatgctc gttatctcga tatccagatt
      541 gtattaaaag ggcaggaagg gatgacattt agcacgcaac cagcaggcgt accagagact
      601 gactggctgg cagataaaga tattgctttt atcgggcagg ggatcgatga aaaaacagtg
      661 attcttaatg aaggggattt tgtcgttttc tatcctggcg aagttcacaa accgctttgt
      721 gcggtgggag cccccgctca ggtacggaaa gcagtagtta agctattaaa atcgtaagtg
      781 atcactgccg ggggcgacct gtgccgccgc ccggcaaaac gttattttgc taatgtagcg
      841 accataacag cttttatggt gtgcatccgg ttttctgcct ggtcaaagac aatacttgcc
      901 ggagattcaa agacttcatc ggtgacttcc attccaccat acagaccgaa ttctgcggcc
      961 atttttttac ccagcgtggt ttgctcatca tgaaatgcag gcagacagtg gaggaactta
     1021 acctgcgaat taccggtcag ggccatcatt ttgctattca cctgatagtc acgcagcagg
     1081 gcaatacgct ccgcccattt ctctttgggc tcacccatcg atacccagac atcggtatag
     1141 ataaagtctg cacctttaac cccagaggcg atatcttccg tcaaggtgat tttacccccg
     1201 tgtttttgtg ccagagcact gcattcggcg accagcgcag cttccggcca gcatgcctgt
     1261 ggcgcgacca aacgcaaatc cagaccggta agcgccgcag cttcgagcat cgaattgccc
     1321 atgttgttac gcgcgtcacc tgcatagacc agcgtcattt cgttgaatgc tttgccgggc
     1381 aaatgctcct gcatggtgag gagatccgcc agcagctgcg tggggtggaa ctcattggtc
     1441 aggccgttcc atactggcac gccagcgtat tccgccagtg tttcgacaat ctcctgacca
     1501 tagccgcgat actgaatacc gtcatacatg cgaccaagca cgcgggcagt gtctttaatc
     1561 gactctttgt gaccaatctg gctgccgctt gggccgagat aagtaacgcg agcaccctgg
     1621 tcatatgcgg caacttcgaa agagcatcgg gtacgggtcg agtctttttc gaagatgagc
     1681 gcgatgtttt tgccagtgag tctggcttct tctttaccgc ttttcttatc ggctttcagc
     1741 ttcgcggcta actgcaacaa gctgttgagt tcagctggcg tgaaatcgag taatttcagg
     1801 aaatgcttat gataaaaccc ggacatagat ccctcctgtg gctaacgcct taatgaatta
     1861 aaattcaatc tatatggatg attattcatt tgcaagtcta aagcataaat ctttgtcaca
     1921 aaggtggagg caatgtcagt ggtgtgtgac aataagagta tcggcaggac attaagagga
     1981 atgagccatg gcaaacccgg aacaactgga agaacagcgt gaagaaacac gtttgattat
     2041 tgaagaatta ctggaagatg gcagcgatcc ggacgcgctg tacaccatcg aacaccatct
     2101 ttccgcagac gatctggaaa ccctggaaaa agcagcagtt gaagcgttta aactcggtta
     2161 cgaagtgacc gatccagaag agctggaagt tgaagatggt gacatcgtga tttgctgcga
     2221 catcctcagc gagtgtgcgt tgaatgccga cctgatcgat gcccaggttg aacaactgat
     2281 gacgctggcg gagaaatttg acgttgagta cgatggttgg ggcacttact ttgaagatcc
     2341 taacggcgaa gacggcgacg atgaagattt tgtcgatgaa gacgatgacg gtattcgtca
     2401 ctaattaagc gttgacgaca aaggctgcgt tacctgatgc gctacgcata tcaggcccag
     2461 gtagttcctg caacctggtg aatttgtagc ccggataagg catttacgtc acatcctgca
     2521 tactcaaaaa gcacgtggtt aatgttcgaa tgcccgcgag tctaagctcg cgggcatttt
     2581 tcaaagtttg cgtagcatcc gtacttcaca atcgacatga cccgtgcagc caagcgcata
     2641 gtcgatatgc tcaaagccca aatgctcata aagcgcaatg gcttccttta aaaaagcggt
     2701 cgtttccaga tagcagcgtt tgaaacccat ttctcgcgcc tgctccattg ccattaaggc
     2761 cagttttttt gccagccctt tgccgcggat agccggaaga aaatacatct tttgcagttc
     2821 gcaaatgtct gactcactcc cggttaatgg cgcaatccca ccgccgccga ccacttcacc
     2881 ctcgtactca accacccaat atgcgtggcc aggctgacta tatacttgat acagctcgtc
     2941 aaggttcgga tcggcgacgg tgtagccttt atcagcggta agaccgtatt cggcggatac
     3001 ctgacgaatg acgtgggcga tagcaggatt atcggcgacg gtaagtttgc gcaaacggag
     3061 tgcgggagaa gcgacaacat tcatagcata gctcaatttc aaatgtatga tttatagctc
     3121 actttaatac caccctcgct taggtggtgc aagaagaaga taaatcagaa aatattacgc
     3181 atatctctct gagataaagc gtattctagg cgaaccagta tcaattgact ggaattatta
     3241 tcggtatgaa tggaatttat tttagggata tagttcatga atgatgtaaa catcgggaaa
     3301 gataattctc ggcactcgtt tgttttcaca ggcaaaggtg gtgagtattt tcttatttgt
     3361 cttgtgaatt ttttactgac aattattact ctgggaatat atgggccgtg ggcattgata
     3421 aaatgccgtc gttatattta tcaacatgtg acactcaagg ggcaaccatt tagttataag
     3481 ggaacaggtg gtgccatttt tgttagcatg ttacttattg tggtggttta tcttctcagt
     3541 atttcttgtt ttgcaggtca acactttgcg ctcggtttat tcctgtttgc tctcttgatc
     3601 tgcggtattc cttgtatggc agtgaagagt ctgcagtatc aggcgaacat gacctcactg
     3661 aatgacattc gctttggctt taactgctca atgatgcgtg catggtgtgc ctgttctgtt
     3721 ggcgttagtt ttttggttcg cactgtatct tattgcacaa gtgacgacat caataggggg
     3781 ggtattcttt aacttagtcg cattaagttt gctttctgcc atcgggctag gtgttgtcca
     3841 tggtataacc tatagtaaat ggatgccatt gctgggtaat aatgcaactt ttggcattca
     3901 taagttttct attcaggtaa acgtaaaaga atgcattaaa ggttgcatgc tcgcaatatt
     3961 aaccatggtt cctttcatca ttgtgattgg tattatgatc gcaccagtat tccagcagtt
     4021 aatgatgatg accatgttgg gtaggtctga tgctggcagt gagtttgtat tacaatatta
     4081 cccgcagatt atggcttctt atttcctgta ttttgtcgcg atccttgttt ttgccagtta
     4141 tctctatgtc acgttacgca atctgttttt gaataatctg acgctggcaa atgggacaat
     4201 tcgttttcat tcatctgtta ccgcgatcgg tatgcttctg cgcatgttgg cggtattaat
     4261 ggggtcctca attacctgtg gtctggctta tccctggctg aaaatgtgga tggttagctg
     4321 gattgctaac aatacccatg tccagggcga ccttgattcg ctggagttaa ccaatgatga
     4381 caaaccgcag gatagcggtt cgttaatgtg gatatctcgc ggtattatgc cgtatgttcc
     4441 gtttatctaa ataaaaaggc cggagcatgc tccggccttc gttttcacta tgagtattac
     4501 agcgcggcga taacagcctg ctgttcaatc agcttcgctt tcgcttctgc atagccttcc
     4561 agcttctcac gctctttcgc gatgaccgct tccggtgcgc gggcgacaaa gccttcgttc
     4621 gccagcttgt tctcaatacg gctgatttcg ccttcgatct tcgctacttc tttcgccaga
     4681 cgtgccagct catcttcttt gttgatgagg cctgccatcg ggatcagcag ttctgcgccg
     4741 tcgacgatct ttgtaacgga aaccggacct ttatcatcag caggcagcac ggtgatgctt
     4801 tccagacgcg ccagcgtttg caggaagccg cggttttcat ttacgcgacg ttctgcatcc
     4861 gcgctgcaac cacgcagcag cagctccagc ggtttacccg gtgcgatgtt catttctgca
     4921 cggatgttac gtacagcaac gatcgcctgt ttcagccact cggtgtcggc cagtgcggct
     4981 tcatcaacct gagacgcatc gtactgcggg aacggctgca gcatgatggt atcggcagtg
     5041 ataccgcaaa gtactttcac tcgctgccag atggtttcgg tgatgaacgg aatgatcgga
     5101 tgcgcgaggc gcagcagacc ttccagtaca gtcaccagcg tatggcgagt accgcgcagt
     5161 tcggcttctg ttccaccgtt cattaccggc ttggtcagct ccagatacca gtcgcagaac
     5221 tggttccagg tgaactcata cagaatgcct gcggcgatat cgaagcggaa gctgtccagc
     5281 gcttcgcggt acgctttgat ggtctggttg aactctgcca gaatccagcg atccgccagc
     5341 gacagcgtca tttcaccgcc gttaaagccg caatcctgac cttccgtatt catcagcaca
     5401 aagcggctgg cgttccacag cttgttacag aagttacggt aaccttccag acgcttcata
     5461 tcccagttga tgtcacgacc ggtagaagcc agcgccgcca gggtgaagcg cagcgcgtca
     5521 gtaccgtgcg gctcaatgcc gttcgggaac tgcttctcgg tgcgcttacg gattttgtcc
     5581 gccagctgcg gctgcatcat attgccggta cgtttttcca gcagttccgg cagcgaaata
     5641 ccgtcaacca tatccagtgg gtcaatcacg ttacctttgg atttggacat cttctggcct
     5701 tcgtcatcac gaatcaggcc ggtcatgtaa acggtgtgga acggcacctg cggtttgcca
     5761 ttttcatctt tgatgaagtg catggtcatc atgatcatgc gggcaatcca gaagaagatg
     5821 atgtcaaaac cagataccat cacgctggtt gggtggaact ggcgcagtgc gtcggtgttt
     5881 tccggccagc cgagcgtgga gaaggtccac agcgcggagg agaaccaggt gtcgagaacg
     5941 tcttcatcct gacgcagcgc aacatcagca ccgaggttat tttctttacg cacttcgtct
     6001 tcgttgcggc caacataaac attacccgct tcgtcatacc atgccgggat acggtgaccc
     6061 caccatagct gacgagagat acaccagtcc tgaatatcgc gcatccagga gaaatacatg
     6121 ttttcgtact gcttcggtac gaactgaatg tcgccattct caaccgcttc aactgccggt
     6181 ttcgccagta catcggcacg cacgtaccac tggtcggtca gcatcggttc gataaccacg
     6241 ccgccacggt cgccgtaagg aacggtcagg tcgtgcggtt taatttcttc cagcaggccg
     6301 agcgcgtcaa ccgcagcaac gacggcttta cgtgcagcaa aacgctccag tttctggaac
     6361 tctgccggga tttcgctgga ataaacgtca gattcgttac ctttggtatc gaacacctgg
     6421 gcgctttcac ggatatcgcc gtcaaaggtc aggatgttga tcatcggcag ggcgtgacgt
     6481 ttaccgactt catagtcgtt aaagtcgtgc gctggggtga tcttcacgca gccagtgcct
     6541 ttttccatgt cggcgtgttc gtcgccaacg atcggaatac gacggttaac cagtggcaga
     6601 atgacatatt tgccaatcag atctttataa cgcggatctt ccgggttaac ggccacgcca
     6661 gtatcgccca gcagggtttc cggacgggta gtcgcaacca ccagataatc tttaccgtct
     6721 gcggttttcg cgccgtcagc cagcggatag cggatgtgcc acatcgaacc tttcgattcg
     6781 cggttttcca cttccaggtc ggagatagcg gtgcgcagtt tcgggtccca gtttaccagg
     6841 cgtttgccac ggtaaatcag gtcttcttta tacagacgaa cgaaaacttc tttcaccgca
     6901 ttggacaggc cttcgtccat ggtgaagcgt tcacgctccc agtcgacgga gttgccgaga
     6961 cggcgcatct gacgggtaat ggtgccgcct gattccgctt tccattccca gattttgtcg
     7021 atgaaagctt cgcggccgta gtcgtgacgg gttttacctt cttctgcggc aatcttgcgc
     7081 tcaacgacca tctgggtagc gatcccggcg tggtcagtac cgacctgcca cagggtgttt
     7141 ttgccctgca tgcgctgata gcggatcatg gtatccatga tggtttgctg gaaggcgtga
     7201 cccatatgca aactgccggt gacgttcggc ggcgggatca tgatgcagaa actttcctgg
     7261 ctttcatcgc cattaggctt aaagtagccc tgcttttccc agtgctcgta aagcggctgt
     7321 tcgatatctt gtgggttata tgtcttttcc attatttcca ggttgccgta ttcaggttga
     7381 aaccagccac gcggtaggct ttatagcgtt cgcgcgccag ttgtttcaga gaatcttcat
     7441 aaggaacgaa gtctaccact tctgtgaaag cggtggcaaa atctgcaaag cttgttcgca
     7501 gactaatcaa tatatcgcgt gggttgctgc tgcgcttttg cggccaggcg atctccaccg
     7561 gcgcaccgcc gcgcggtcct tctcccgcta aattatgcgg aacaaagctt tctgccggac
     7621 gcgcccacag ggcttcatcc agtcggtaag cctgcttttc atcttcacag gcgatgagta
     7681 cgcgcttacc actgcgccaa cgttctgcgg caatttcaca caccaactgc tcaacggcgc
     7741 ttaagccatc gacggtggtg tcattgtcca gaaggtagaa cgttgcgttt ttcatatatg
     7801 gggcttcttg tggtggattt aaatacgcat caggtataca ccgcagcgcc tgatgcgacg
     7861 ctgccgcgtc ttatcaggcc tacaagttaa gtgctgtgtg taggtcggat aaggcgttca
     7921 cgtcgcatcc gacaataaca gccttgcctg atgcgacgct gtcgcgtctt atcaggccta
     7981 cgagttcagt gctgtgtagg tcggataagg cgttcacgcc gcatccgaca ataacaacac
     8041 agcctgatgc aattactctt cgccgttaaa cccggcgcgg tttagcagga actgtgccag
     8101 caacgccacc gggcgaccgg ttgcgccttt cgccttacca gaacgccagg cagtaccggc
     8161 gatatccagg tgcgcccagt tatacttacg ggtaaagcgc gacaggaagc aacctgcggt
     8221 aatcgcccca ccaggacgac cgccaatgtt cgccatatcg gcaaaattgg actccagctg
     8281 ttcctggtac tcgtcgccca gaggtaagcg ccatgcacgg tcaccggatt gttcagacgc
     8341 ggcaatcagt tcatgggcca gtgggttatg gttcgccatc agaccggtaa tgtgatggcc
     8401 tagcgcgatc acgcaggcac cggtcagcgt cgccacgtca atcaccgctt ccggctcaaa
     8461 acgctcaacg taagttaaca catcgcacag taccaggcgg ccttcggcgt cggtattcag
     8521 cacttctacg gtttgaccgg acatggtggt taacacatcg cccggacgat aggcacgacc
     8581 gccaggcatg ttttcgcagc ctgccagcac gccgataacg ttaatcggca gttgtagctc
     8641 cgcgaccata cgcatcacgc cgtagaccgc tgccgcaccg cacatatcgt acttcatctc
     8701 atccatgcct tctgaaggct tgatcgagat accgccggag tcgaaggtta aacctttacc
     8761 caccagcacg attgggcgtg catcttccga cgcgttgcct ttgtactcaa tcactgacat
     8821 cagcgattcg ttttgcgaac cctgaccgac cgccagatag gaatgcatcc ccagctcttt
     8881 catctgctgt tcgccgataa ctcgggtgat gacattcttg ctgtagctgt cagccaactg
     8941 gcgcgcctga gaggcgaggt aagcagcgtt acagatattc ggtggcatat tgccgagatc
     9001 tttcgcggct ttaatccctg cggcaatcgc cagaccatgc tggatcgcgc gttcaccgct
     9061 ggtcagctca cggcgggtcg gcacgttgaa caccatttta cgcagcggac gacgcggttc
     9121 gctcttgttc gttttcaact gatcgaaact gtagagcgtc tcttttgccg tctcgacagc
     9181 ctgacgcact ttccagtagt tgttacggcc tttaacgtgc agttcagtca gaaagcagac
     9241 cgcttccatt gagccagtat cattcagcgt attaatggtt ttctgaataa cctgcttgta
     9301 ctgacgctca tccagctcac gttctttgcc gcaaccaata aggagaattc gctcggaaag
     9361 tacattcggc acatggtgca gcaacaatgt ctgccccggt tttccttcca gttcgccccg
     9421 acgtagcagg gcgctgatgt acccatcgct gattttatcg agctgttctg caatcggaga
     9481 aaggcgacgt ggttcgaaga cgcccacgac gatgcaggca ctccgctgtt tctccgggct
     9541 accgcttttt acactaaact ccatgcacta cgctcctgaa tcttaaagac aacggcggtg
     9601 gctacagata gaattgcaag ctttcgtaac tcatgtccgc tgttgcgatg acttcgtgtt
     9661 aatcttaacg ttattacggc attggcacgt cagaacaaaa tctgagaggt gaatccgttg
     9721 agtataatta tcttagcgac gatttcgacg actcaagaga ataaatgacg tttaagccat
     9781 gaaacaagct aaaatcctgc aaaagacgag tttttacggg cgtatttaaa gtgataatca
     9841 taagatatct ggtgcgggag acgctcaaaa gccagctggc gatactcttc atcttgcttt
     9901 tgatcttctt ctgtcaaaag ttagtgagga tcctcggcgc agcggttgac ggcgatattc
     9961 cggcgaacct ggtgctctcc cttctcgggt tgggcgtgcc ggaaatggcg cagcttatcc
    10021 tgccattaag cctgttcctc gggctgctga tgacgctggg caaactgtat accgaaagtg
    10081 aaattacggt aatgcatgcc tgcggtctga gcaaagcggt gctggtgaaa gcggcaatga
    10141 tccttgcggt gttcacggca atcgtcgcgg cggttaacgt gatgtgggcg ggaccatggt
    10201 catcgcgtca tcaggatgaa gtgttagcag aagcgaaagc gaaccctggc atggcggcgc
    10261 tggcgcaagg gcaattccag caagcgacta atggcagctc ggtgctgttc atcgaaagcg
    10321 ttgacggcag cgatttcaaa gatgtgttcc tcgcgcaaat tcgaccaaaa ggtaatgcac
    10381 gaccttctgt ggtggtggcc gattccggac atttaaccca gctgcgcgac ggctcccagg
    10441 tcgtcactct caaccaggga acgcgcttcg aaggcactgc actgttacgt gatttccgca
    10501 ttacggattt ccaggattat caggcgatca ttggtcacca ggcggtggcg ctcgacccga
    10561 acgataccga ccagatggac atgcgcacat tgtggaacac cgacaccgat cgtgctcgcg
    10621 cagaactgaa ctggcgtatc acgttggtgt tcaccgtgtt tatgatggca cttatggtcg
    10681 taccgctgag cgtggttaac ccacgtcagg gacgcgtact gtcgatgctg ccagccatgc
    10741 tgctgtatct acttttcttc ctgatccaga cctccctgaa atcgaacggc ggtaaaggta
    10801 agctggaccc gacgctgtgg atgtggaccg ttaacctgat ttatctggct ttggcgattg
    10861 ttctcaacct ttgggacacc gtgccggtcc gccgcctgcg cgccagtttt tcgcgtaaag
    10921 gagcggtgtg atgcaacctt ttggcgtact tgaccgctat atcggtaaaa ctattttcac
    10981 caccatcatg atgacgctgt tcatgctggt gtcgctgtcc ggcattatca agtttgtcga
    11041 tcagctgaaa aaagccgggc aggggagtta cgacgcgtta ggcgcaggaa tgtatacctt
    11101 gctgagcgtg ccgaaagatg tgcaaatctt cttcccgatg gcggctctgc ttggggcgtt
    11161 gcttggtctt gggatgctgg cgcagcgcag cgaactggta gtcatgcagg cttctggttt
    11221 tacccgtatg caggtggcgc tgtcggtgat gaaaaccgcc attccgctgg tcttgctgac
    11281 gatggcgatt ggtgaatggg tcgcgccgca gggcgagcag atggcgcgta actaccgtgc
    11341 gcaggcgatg tacggcggct cgttgctctc tacccagcaa ggtttatggg cgaaagatgg
    11401 caacaacttc gtctacattg agcgggttaa aggtgacgaa gagttaggtg gcatcagtat
    11461 ttatgccttt aacgagaatc gtcgtctgca atccgtacgc tatgccgcta ccgcgaagtt
    11521 tgacccggaa cataaagtct ggcgtctgtc gcaggttgat gaatctgacc tgaccaatcc
    11581 aaaacagata accggctcgc agacggtgag cggcacctgg aaaaccaacc tcacgccaga
    11641 caaactgggc gtggtggcgc tggacccgga tgcactctct atcagcggtt tgcacaacta
    11701 tgtgaagtat ctgaagtcga gcggtcagga tgccggacgt tatcagctca acatgtggag
    11761 caaaatcttc cagccgctat ccgtggcggt gatgatgctg atggcgctgt cgttcatctt
    11821 tggcccactg cgtagcgtac cgatgggcgt gcgtgtggtc actggtatta gcttcggttt
    11881 tgtcttctac gtactggacc agatcttcgg cccgctgacg ttggtttacg gcatcccgcc
    11941 gatcatcggc gcactgttgc caagtgccag cttcttctta atcagcctgt ggctgttaat
    12001 gagaaaatcg taacctgatg aacgaataaa gggagcgaaa gctcccttta ttattgttag
    12061 caaagcgtgc ttcgttcatg cctgaaaaat catgaaaatt caatatattg cagtggtgtg
    12121 ggcatgataa ggcgcgccag cgtcgcatca gacaaagaca aatcgcacct ggcttacctt
    12181 cttctccccc ccagcaaact ccccaacatc ccgcgcacaa tctgattcgt cacctggcgg
    12241 gcggcgcttt ttgccattgt ttgcaccaca ccatctttct tcccgccgcg tggtccagta
    12301 gtgccaaaca aaatatcctt caatccaccg agaataccgt catccaccgc cacctctttc
    12361 cctttcgcgg gaggattatt ttgctgctcg atactggcct gaaagccttt ttgcaacatc
    12421 tcataggcgg attcccggtc cacgtcatcc tcgtacttgc catacaccgg ggagtggtta
    12481 atcaagccat tgcgctcatc ttccgtcacc ggccccattc gcgaacaagg tgcgatcacc
    12541 atcgcccgtt ccaccacaga aggacttcct tttgcatcga gaaacgagat taacgcctcg
    12601 ccggtcccca gttcctggat tgccttttcg gtatcaaatg ccggattggc ccgcatggtt
    12661 tgcgccgcag cttttactgc tttctgatct ttgggcgtaa aagcccgcaa agcgtgttga
    12721 acacgattac cgagctgccc gagcacatta tccgggatat ctgacgggtt ttgcgaaacg
    12781 aaccagacgc ccacgccttt tgagcgaata agccttatca cctgctcaat cttatccagc
    12841 agtacctgtg gcgcatcgtt aaacagcaga tgtgcttcgt cgaagaaaaa caccagtttt
    12901 ggtttctcca gatcgcccgc ttcaggcaat tgttcatata actctgaaag catccacagc
    12961 aggctggcgg cgtacagttt cggcatctga taaagtttct cggcgctgag gatattgata
    13021 acgcctttac cgttggcatc ggtgcgcatc cagtctttga tatccagcat cggctcacca
    13081 aagaagtgtg ctgcgccttg ctgttccagc gacagtaatc cgcgctggat ggcaccaacc
    13141 gatgcgctac tgatatttcc gtactgattc tggaaggatt tggcgttatc gccgatgtac
    13201 tgggtaatcg cccgcaaatc tttaaagtcg agcagcaaca atccctgatc gtccgcaatg
    13261 cggaagataa tattcagcac gccagattgc acatcgttga gattcaacag tcgcgccagc
    13321 aacagcggcc ccagatccga aaccgtcgcc cgcaccgggt ggcctttctc gccaaagata
    13381 tcccacacca ccaccggatt agcatgaggt tgccagtcat ttacgccgat atttttaagc
    13441 cttgcgagca gtttttccga agaagtacct tcctccgcta ctcccgttaa atcacctttc
    13501 acatcggcca taaacaccgg cacgccgatt tcagatagag actctgccag tttttgcagc
    13561 gtaacggttt tacccgtccc cgttgcgcca gtaatcagcc cgtgacggtt agccattccc
    13621 ggcagtaaaa acagttctgt atccggcgtg cgggcaatta acaggggttc gctcatgaga
    13681 tttcctccag tttcctgcct ggagtatagg caacgtccgg caaagagaat atgtgtaatg
    13741 cagagaaaat aacctaaagc gtgttaccgt gataaatctg atacccgagg tcaacagtgt
    13801 tgtggttgtg atcgttattc ttaattttgc tcagcaacat ttgggcggcc atccgaccaa
    13861 tatcaaaacg gggagtaatt acgctggcga ggcttgggat catttgcctg ccaatctcca
    13921 gcccatgaaa acccgcaatg gaaatttgtt ctggcacggc gaggtttcgc tcgcggcaca
    13981 gcagaagcgc acccatcgca atatcgtcat tggtgcaaaa tacaccgtct aaatccgggt
    14041 ttgcgctaag ggcatcacgc attaactgca ttcctaaatg aatggatgag atggcgcgtg
    14101 gattcatgcg taatggggaa agattatgca gcatcatcgc atcgcaatac ccctgataac
    14161 gctgttcatc gcgggtgtca tctttggaac cgagatacag aattttgcgc ctgactcgct
    14221 tatccagcat ggtacacacc atgtcaaaag cagcctgccg attatcaaaa ccgacctcca
    14281 tatccagccg ttctccctgt acatccatca attccacgac cggaatggtg gctgaacgca
    14341 gaaatttcac cgtcctgatg gtgtgatatt tttccgaaag aataatcccg tcaatgttat
    14401 aagagagcag gttaatcacc gactcttctt cggaatcgcg atcgtaattg taattagcaa
    14461 taagggtctg ataattatgc acagaggtga cggattctat tcccgcgaga atgtcagcga
    14521 agagttgatt ttgaaatgac ggaatcaaga taccgagggt ataactttgt gcattcaaca
    14581 acatgccggg agcgcgatta gggatataat tgatctcttc cataattttg gcgatgcgct
    14641 cgcctgtttc ctttgccacc tttttcggcg agcggatata acgactcacg gtcatttttg
    14701 ttacgccagc cagcgtagcg atatcctgta aagaaattct gtgattgcgc ataacttctc
    14761 ccaacgtcgg gggaatcttc aatcttcccc cgaaactaca tcacaatatt ttattctttc
    14821 agtgcaatac ggcgttaatg gctaacacgc ccagcaaacc cataatagaa ataagggttt
    14881 ccatcaccgt ccaggtacgc aacgtttcac caaccgtcag attaaaatac cctttaaata
    14941 gccagaagcc agggtcgttt acgtgtgacg cgatcacgct gcccgcaccg gttgccagta
    15001 ccattaaagc gggatcggca tgggtaacgt tgataatcgg caacaccaca cccgcggtgg
    15061 taatggccgc gacggtggca gagcccagag cgatacgcaa cagcgccgca acagtccagc
    15121 acatcaataa cggcgaaagt gtagttccgg tcattaagtg tgaaatatac tgcccgacac
    15181 cgctatctac taatacctgc ttaaacgcgc cgccgccagc gataataaac acaatcatcg
    15241 cgatagcgcc tatagaatcc ccaatgatat ccatgatttg ctcgatggtg cgtccattac
    15301 gtcggcccag tgtgaaaatc gcaataacaa tggcaataaa cagcgcaacg gcagggttac
    15361 cgacaaattc aaagaagagg cgcacggtgt tagttttcgg taacgtaatt tcacaaacgg
    15421 cggcgatagc catcaggatg accgggatca cggcagcgaa aatactgttc cagaaggagg
    15481 gcatctcctc ttcgctaaac agatgaggat taaataagcc ttccggtggt gctttctcaa
    15541 agcgagttag cagtttagaa aacagcggtc ctgcgacaat aactgtcgga atggtaatga
    15601 taaatccata cagtaaagtc gttccgagat tagcctcaaa gatagtcgcg atggcagtag
    15661 gccctggatg tggcggcaga aaacagtggg ttacagagag cgctgctacc attggtacgc
    15721 caacatacaa caggggtaat cctgatgatg ctacgatggt aaataccaac ggcaacagca
    15781 ggacaaaacc cacttcaaaa aacatggcga ggcccacaac cagaccggtg atcactagcg
    15841 cccattgcac gcgtttttta ccaaaagtag caatcagcgt agtggcgata cgttgtgccg
    15901 cacccgtatc agaaatcagc ttgcctaaca tggccccgaa accgaggatc attgccagcc
    15961 cgccgagcgt gctgccgata ccattttgta tagagtgcag gacggcctgt gcatccatcc
    16021 cttcggcaaa tccgacgacg gcagcgacca gaacgagggc aataaagccg ttaactttaa
    16081 agccgatcat caggataaga agcagcgcga cgcctgccgc aataatgatt aatggcatag
    16141 tctttatctc ttattagtgc cagaaaagca gcgctttcgc gctgcacgta gggtagtagg
    16201 tgttaaacag ccactaacat gccgccatca acaaacaaca ggtggccgtt tacgaaatca
    16261 gaggcttttg aagaaaggaa caccgcagca ccaatcagtt cctgcggatc tccccagcgt
    16321 gcggcggggg tccgtttgca caaccaggcg gtgaaggctt cgtcctcaac cagtgcttta
    16381 gtcatttctg ttttgaaata gcccggcgca ataccgttga cctgaatatt gtggcgcgcc
    16441 agctcgacgc acatgccgcg ggtgagcatt tttaccgccc ctttcgatgc ggcataaggg
    16501 gtgatggtgt cacgtcccag ttcgctttgc atcgagcaaa tattaataac tttacctgcc
    16561 ttgcgttcaa ccatgtgacg agttaccgct tgcgatacca ggaacacggc ggtctggttt
    16621 actgcgatca catcattcca ctcttgttca gggaactcag taaaaggatg acggcgctgg
    16681 ataccggcgt tattcaccag cacatcaatg gggccgatgt ccttttcgat atgttcaacg
    16741 gcggcatcaa tttcatgttt atgagtaaca ttaaaaggtg cggcaacggc ctgaataccc
    16801 tcctggtgga gtttttctac agcaagttct gcgcgttcgg cagtaatatc attaataatt
    16861 atttgtgcgc catatttacc caggccggtt gccagtaaaa agccaatgcc ctgtgctgaa
    16921 ccggtaatca agatattttt tcctgccagt gaaaatagat cgttcattgt attattcctt
    16981 tatttatctt ttagaaaaca agctggactt ttgctgcctg ggttttatca ccggcgaaac
    17041 gtagcgcctc ttccaggtct gtgaagggat attcagcact cagtaaaggc agtggattaa
    17101 taacgccatt cgccagccat gacactgcgg tattaaattc gctggtaaaa cggaaagagc
    17161 ctttgagtga aatctcctta ccaatcaacg tcatcattgg gaattctgcc atcgcgcctc
    17221 ccatccctac ctgcaccatt acgccgcgtg cacgagtgac ctccagacag gtattcactg
    17281 atgaaggatg accggacact tcaaagctga catcgaaata gcctttttcc gctttccagt
    17341 gatccatgtc gtcgttttgt gggtttacga gcacatccgc ccccatctct ttgcccagcg
    17401 aaagggaacg gggactcaca tcggcacaga caatttccgc ggcccccagt gttttcacag
    17461 cactgacaat caggcagcca atgggcccaa caccagaaat aaatactcgc ttgccctgta
    17521 actcgccggc ctgatgtgcg gcatgaatcg cgacggctaa aggttcggca aaagccataa
    17581 ccttttcgtc agctttggcc ggataaggga cacattgcga cgtttcgacc attttataac
    17641 gggtaaagcc accatcaaca tgagggaaat acatggcact gccaaaaaaa cgcatctctg
    17701 tacactgatt ctcgttatgt tcaatgcagt atttgcagtg accgcacggt ttagacggat
    17761 taatggctac cgtttgccct tcatgtaatt ttgatgagtc gctatgaata actttaccga
    17821 taacttcatg acctaacacc atcggtgcct ttatcatgaa attacctact tttccttcct
    17881 gataataatg taaatcggaa ccgcaaattc cacctcgggt tatttgtact aatgttccat
    17941 tattattcca atctatcgtc tgctcggtaa cggcaacagt tttcttgccc gcaacaacgc
    18001 aggactgtgt tttcacttgc ataaattcct cactggtcag gtagacacct cggaagcatt
    18061 taagcggttt taattgtcat tatttgtgat gaagatcacg ttagaaaatt gttacattac
    18121 gatgttacgc ataacgtgat gtgccttgta attcttatca gcagaaataa aaaaacgtga
    18181 aattattatg ccaccaggcg tagtatcgca gcaggtaaga tgattcagga gattttaaat
    18241 ggcgggtgaa agctttattt tgatgggcgt ttcagggagt ggtaaaacat taattggtag
    18301 caaggttgcc gcgttattat ctgctaaatt tattgatggt gacgatcttc atccagccaa
    18361 aaatatagat aaaatgtcgc agggtattcc attatctgat gaagatcgac ttccctggct
    18421 ggaacgctta aatgatgctt catacagtct ttataaaaag aatgaaacag gatttattgt
    18481 ctgttcatca ttaaaaaaac agtatcgtga tattttacgc aagggtagcc cccatgttca
    18541 tttcctctgg ttagatggcg actatgaaac tattctcgcg cgaatgcagc gtcgggctgg
    18601 gcattttatg ccggtagcgt tactaaaaag tcagtttgag gcactggagc gtccacaaga
    18661 cgatgaacag gatattgtgc gcattgatat caaccatgat attgccaatg tcaccgaaca
    18721 atgtcggcag gctgtgctgg cgatacgaca aaacagaata tgtgcgaaag agggcagcgc
    18781 ctcagatcag cgctgcgaat gattttcaaa aatcggcttt caacaccacg cggtaacgcg
    18841 ccttaccgtc gcgcacatgc tggatggcgt cgttaatttt cgacatcggg aacagttcgg
    18901 tagtcggcgc aaccttgctg cgggcggcaa agcgcatcag cttacgcaac tcataaggtg
    18961 taccggtagc agagccagag acactgcgat cgcccgcaat cagcgtaaag gccggaaccg
    19021 gcaacggcgt gagaaccgca ccgacggtgt ggaaattacc gccatatgtc agcgcctcaa
    19081 aataaggctg ccagtcgagg ctgacgttca cagtattgat aatgagatca aactgccccg
    19141 ccagagtttt cagtgcctgc ggatcgcggc tattcaccac tttatcggca cccatcgcca
    19201 gtacttcctg ctctttcgcc ggatttgaac taaaggctgt cacctcgcat cccattgcgt
    19261 gcagaagttt tatagcgata tgtcccagcc cgccaatacc aattacccca acgcggctgg
    19321 tagcagtgat atggtgcatc aacagtggtt taaagaccgt gataccacca cacagcagcg
    19381 gcccggcgga ctcgatatcg atattttccg gcagtgggat cacccattgc cagtctgcac
    19441 gcaacttctc ggcaaaaccg ccgcgattca taattgttgg caccgcacct tgctcgcagt
    19501 tgatctgatt accgctaata caggcatcgc aatgcccgca gctacgcgcc gtccagccaa
    19561 tcccgacacg ctgaccgacc tgcaaacctt tatcctgcgc ggcgctcccg agcgccacca
    19621 cgcgaccaat cacctcatgc ccggcaatca gcggatattg tgaaaatccc cattcattgt
    19681 cgatcatcga tagatcggaa tggcagatcc cgcaataatc cacctgcact tcaacatctt
    19741 gtggcctcag ctcaccggga tcgtactcat aaacttccag ttcgccgccc gcttcttttg
    19801 cggcatagct ttttatcatc gacatttttt ggtccttctc tggtgttgtc tgggaagtgt
    19861 agagcatggc agggcggggt gcctggagtg tgacaaaggt tacacatcgc ggtatgcaat
    19921 gctgaaaatt tcagcactta gcgaggtgcg agcaagctgg cgcttgcatg gtggcgtgcg
    19981 acaggtataa tccacaacgt tttccgcata cctcttcagt gccgaagtgg cgaaatcggt
    20041 agacgcagtt gattcaaaat caaccgtaga aatacgtgcc ggttcgagtc cggccttcgg
    20101 caccaaaagt atgtaaatag acctcaactg aggtcttttt ttatgcctga aatccagtgt
    20161 ttatctatct ttcccgctat attaactctc tcaaggtcaa ccgatatcaa cgtacatcta
    20221 ccaacatatg ttggtacaga tgatggtatt tccggttcga taatgcttgt accaacaggg
    20281 agggaatacg catggcatta acagatatca aagtcagagc agccaagcca acggataagc
    20341 aatataagct gactgatggt ggcggtatgc atctgcttgt ccatccaaat ggttctaagt
    20401 actggcgttt gcagtaccgt tatgagggaa agcaaaaaat gctggcactt ggggtttatc
    20461 ctgaaatcac actagcggat gccagagtac gtcgtgacga ggcgcgtaac ctgcttgcga
    20521 atggcgtcga tccgggagac aaaaagaaaa atgataaggt tgaacagagt aaagcacgaa
    20581 cctttaaaga agtcgcgatt gagtggcatg gcaccaataa aaagtggtct gaagatcacg
    20641 cccatcgtgt gctaaaaagt cttgaagata atctttttgc agcgcttggt gaacgtaata
    20701 tcgctgagtt aaaaactcga gatttattag cacctattaa ggccgtagaa atgtctggac
    20761 gtcttgaagt ggccgctcgt cttcagcagc gcactacagc catcatgcgc tatgcagtgc
    20821 aaagtgggtt aattgattat aacccggcac aagagatggc tggggcggtt gcttcctgta
    20881 atcgacaaca tcgtcccgcg cttgaattaa agcgcatccc tgagttgctt acaaaaatag
    20941 atagctatac tggtaggccg ctaacccgat gggcgataga actcactttg ctgatcttta
    21001 ttcggtccag tgagctgcgt tttgctcgtt ggtcagagat cgatttcgaa gcgtctatat
    21061 ggactatccc accggagcgg gagcctattc ctggagtgaa acattcccat agaggctcaa
    21121 aaatgcgtac aacgcatcta gtgcctcttt caacgcaagc tcttgcaatt ttaaagcaga
    21181 taaaacagtt ttatggggcc catgacttga tatttattgg tgatcacgat tcgcacaaac
    21241 ccatgagtga gaatacggta aatagtgcgt tacgggtcat ggggtatgat acaaaagtag
    21301 aggtttgtgg tcatggcttt cgaacaatgg cctgtagttc attggtcgaa tcaggtttgt
    21361 ggtctcgtga tgctgttgaa cgtcagatga gccacatgga gcgaaattca gtgagggccg
    21421 cgtatatcca taaagcagag catctggaag aacggcgatt gatgctacag tggtgggccg
    21481 attttctgga tgtaaacaga gaaagattta tcagtccatt tgaatatgca aagattaata
    21541 atccattaaa acagtaatca tcccgggcaa atgcccggga attattctag gattattttc
    21601 tttgttaaaa aagacaaacg gtattaactg atgtatttac tatttaccgc tccctgcagg
    21661 ggggattatc agaatccgcg gttattcatt atttatatac tttcaataaa aaagtaacct
    21721 cataaaaaat cctgtactca caggatgatg cgggataact gacgtaagga gtgctgcagg
    21781 gatttgatgg tcccttgtgc tgatatgaat acggatattt tatacagaat acttttcagt
    21841 acaagcgact gtattacagt ctgccatata tgattacctg tgatcaggaa gaataagtgg
    21901 ctgaggtttc aaactgtctg ccggtatatt cctctctccc ttaaaaacca taacagttta
    21961 attatcttcg tctgtcagcg gattgaatgg cggtatattt tcagtacaaa taccggtcat
    22021 ccactgaaaa atacctgcga aatgacgggc actgattttt ctactgacgg gctgatgaga
    22081 cgttatgtca ctgacatata atccctgcgc acactctcac cagtgacaat aacaatcgta
    22141 tcatataacg gtgctcccgg ctggattttc cagttgtcag ccccgtgctg actcagttgt
    22201 tcataacgct gcatttcacg taatgtgtca gttgtcccca caactgttcc tttaaccatc
    22261 cgcaatggcc aacggtttac agagcataat acgaatagcc gtagagccag ccagttacgg
    22321 tggtcgcgat ggtgtgtccg ccagaaaatc accacgaata ccagaatcgc ggcactgacc
    22381 agaaaatgat aaacaggaaa cacggggcgg ttcactttgc tttctcctgt agtgttttct
    22441 gtaggttctg aaccggttcg agcggggttg tgaggctgat ggcaatcccc gggctcttcc
    22501 tgcatgttac actggtactg caagaggggg ccttcgctcc tctgacatta ctcagtattt
    22561 tcactactac accccatctg ccatccataa caaccgattc accccctcgc gaggcgttgt
    22621 tgcccgtcac ccggacagac atcgttatgg acttctcttg ttgcacgatc gatcaattgt
    22681 tatgcatgct gacaccacaa ccccggtggg tatgaccgat gcctttctca ctctcttccc
    22741 ggtcatcgtc ggccttcccc gttattgtgg agtgtcagca ccctcgacgg catttcgatg
    22801 gctgctcagt gttcactcac gttacggccc gcataacccg ttgacttcct gaagaagtcg
    22861 tttcagggag agattcagcc cttttgttac ctcctggacc gcttcttgtc cttccagtcg
    22921 gagcgagagt tagccggggc ggactttcac cgacggaaag atcgtgcatt tcaaggcaca
    22981 cacaacaata tctgcgagcg tgctatccgt ccggtcgtta tgggacgaaa ggcctggttg
    23041 ttcgctggtt cactgatggc cgggaaccgc gcggcacaga taatgagcct tctgggaacc
    23101 gcagtctgga gccacatgct tggctgacgg acgtcctgac gcgtctgccg gagtggccgg
    23161 aggagaggtt ggctgagtta ctgcctcttg agggctttac cttcttcggg taagtgatac
    23221 ctgccgtcag gtgtttgtgc accgggccat aacctgcagt tgggaattga acgcctgaca
    23281 acaggaaatg agctagagca gtaaacacgc gggctgacaa tgggtcagaa tatgagtcag
    23341 cccggcgtta ttcctggggt cagtacttca gatccagcct ccggtaccag actagctagc
    23401 ccctacgcaa ccaaataaat caaccatcgt cagagtgcaa tcgttcatat acactgtcgg
    23461 tgatgcgctg aaaaatgcgt taatgttgag ttcctcgaac ccttaccaat taagggacat
    23521 agccagatac gcaactggct gatgcaagca gtctaatgtc tttagatccg ctggcctgcg
    23581 ggcatgacaa tgtcaaacag tcaaagattg tggcgttgac ggtcagtctt ggggacaaaa
    23641 aagctcagta ttctcgttgg cagccagata atttaacagt tgatgctgaa acgaaatagg
    23701 catcttggtt ctggtgtttg taacaaacta tcagctacaa aaatatgctc aatttgtgac
    23761 atcagtaaca aaacgcgttt tgttatgtgg attgcttgtt ttttgatctg gtataacagg
    23821 tataaaggta tacagaaaag caaagaaata ctgcaaagga aaacagctat aacgtaagct
    23881 aaagtaataa cctctcagtc tttcctcatt tgactaaggg agttttattc aacctgaacg
    23941 gactacgaaa atgagcacaa tgaataagtc aatttttgaa gcagggttga actgtgccag
    24001 tggtggaata tctggcgaac attgtttttt gttggtgacc caaaactgta ggtgggtatc
    24061 agttttatct ttcatagagt gaaatatgtt aagaagaaat ggaggaaaag attgtacgga
    24121 ttaggtattg ataacaatca atagtactgg cgtatttgaa gacaatataa ttatttctgg
    24181 atattgttga ggctccctaa tatttacttt aagggctata ttagaataac acaggaaaca
    24241 aatatggcaa cagcatggta taaacaagtt aatccaccac aacggaaagc tcttttttcc
    24301 gcatggcttg gatatgtatt tgatggcttt gattttatga tgatatttta cattcttcat
    24361 attataaaag cagatcttgg cattacggat attcaggcta ctttaatagg gacagtggcc
    24421 ttcatagcca gacctattgg aggtggtttt tttggtgcca tggctgataa atatggtcgt
    24481 aagccaatga tgatgtgggc aattatcatt tattcagtcg gaacaggcct tagcggtatt
    24541 gctacaaact tatatatgct cgcagtttgc cgttttattg ttggcttagg gatgtctggt
    24601 gaatatgcat gtgcttcaac ttatgcggta gaaagttggc ctaaaaatct tcaatctaaa
    24661 gctagtgctt ttttggtaag tggtttttct gttggaaata ttattgcggc acaaataatc
    24721 cctcagtttg ctgaagtata tggatggaga aactcttttt ttataggcct gttaccagtt
    24781 ttactagttc tttggatcag aaaaagtgct ccagaaagtc aggagtggat tgaagataaa
    24841 tataaggata aatcaacttt tttgtctgtc ttcagaaaac cacatctttc aatctctatg
    24901 atcgttttcc tcgtctgttt ttgtctattt ggtgcaaact ggccgataaa cggactactt
    24961 ccttcctacc tggcagataa tggagttaat acagtggtta tttcaactct gatgacaata
    25021 gcaggtttag gaacactgac aggtacaata ttttttggtt ttgttggtga taagattggt
    25081 gtaaaaaaag cctttgtagt cggtctaata acttcatttg ttttcctttg tcctcttttt
    25141 tttatttctg tgaaaaactc ttctcttata ggattatgtc tctttggatt aatgtttaca
    25201 aatttaggta ttgcagggtt ggttccaaaa tttatatatg attactttcc aacaaaatta
    25261 agaggattag ggaccggtct tatttataac ttaggggcaa ctggaggaat ggccgcacct
    25321 gtattagcta catacatttc aggatattat ggcttaggtg tttcattatt cattgttacg
    25381 gttgcattct ctgccttatt aattttgtta gttggttttg atattccagg taaaatttat
    25441 aaactatccg tggctaaata attggaggct ttatgattaa ttatggcgtt gttggtgttg
    25501 gatactttgg cgctgaatta gctcgtttta tgaatatgca tgataatgca aaaattacat
    25561 gtgtatacga tcctgaaaat ggagaaaata ttgcccgtga attgcagtgt atcaatatgt
    25621 caagcttgga tgctttagtc tcaagtaaat tagtcgattg cgtgatcgta gccaccccaa
    25681 attatctgca taaagaacca gtaattaaag cagcaaagaa taagaagcat gttttttgtg
    25741 aaaaaccaat tgcattaagt tatgaagatt gtgtggatat ggtcaaagcg tgtaaagaag
    25801 ctggtgtgac ctttatggcc gggcatatta tgaatttttt caatggggtt caatatgcac
    25861 ggaagttaat taaagaaggt gttatcggcg aaatattatc atgtcatact aagagaaatg
    25921 gctgggaaaa caaacaagag agactttcct ggaaaaagat gaaagaacaa tctggtggac
    25981 atctatatca tcatatacat gagttagatt gtgttcagca tttacttgga gaaataccag
    26041 agacggttac tatgattggt ggaaatttgg cccattctgg tccaggattt ggcaatgaag
    26101 atgatatgtt atttatgacc ttggaattcc cgtcaggaaa actagcaacc ttagagtggg
    26161 ggagtgcatt taactggccg gaacattatg tcatcatcaa tggaactaaa ggctctatta
    26221 aaattgatat gcaagaaaca gcagggtcac ttaggattgg cggtcagaca aagcattttt
    26281 tggtccatga aacacaagaa gaagatgatg atcgtcggaa aggcaatatg acctcagaaa
    26341 tggatggcgc tatagcatat ggtcatccag gaaaaaaaac accattatgg cttgccagtt
    26401 taataagaaa ggaaacgtta ttcctccata atatcctctg tggtgcaaaa cctgaagaag
    26461 attatattga ccttctcaat ggtgaggcgg ccatgtcggc gattgctact gctgatgctg
    26521 ccactctttc aagatcgcag gacaggaaag tgaaaatcag tgagatcatt aaacatacat
    26581 cagtaatgta aaaactatac gcctcttgat ttaattcagg aggcctttta tgtatgatca
    26641 agaattttat cttcgataat ctcataattt tagcagtgcc atttatgata aaaacatccc
    26701 ttaaaaccaa tctaatcttt ttcttcctct gcgtatttgt tccccgtatg gcgtcataat
    26761 ttacgctacg taatacggga tagttacgat acgcagcgat agcgctaagt tttagttaaa
    26821 atcccccatc agcgggatat ggggatctct gggaggttca ggttttagca tcgcgattaa
    26881 tacgacagac gctggtgacg atgatgccaa gtagtggctc ttccgaataa ggttcccact
    26941 gaccaattgg cgcgcgattc gcaataacat ctcccttcgc ccagtattgg gaaaagtaga
    27001 tacattcaaa ctgtgtacgc tgtttcgtct cacagttcac gacattgctg gccgatgagc
    27061 tgaccattgg gcctatccgg gctgtcccga tggatattac gccgaagtat gtgggaattg
    27121 ccagcggatt gatgaacgcc ggttccgctg tcgccgacat tatttcacct atcgcctttg
    27181 gcattattat cgataaaaca ggcaactgga gtttaccatt ctacggttcc gtcgcattgc
    27241 ttgttattgg tatattcctg acgttcttca tgcgtccaga taagtcattg taaaagtcga
    27301 attcagggct gaagtggtct actgaatttg gccatctgaa cagggtgata tgctcacctt
    27361 agaacaacac aggtgcacca atgaaaagaa gaaatttcag tcctgaattc aaacgtgaat
    27421 ccgctcagtt ggttgtcgat caaaattaca ctgtttctga cgctgctaag gctatggatg
    27481 ttggtctttc cacgatgacg gaatggttca aacaactgcg tgatgagcgt cagggcaaac
    27541 gccaaaagcc ttcctgataa caccggaaca aatcgaacta cgtgagctaa agaaaaagct
    27601 acaacgaatt gaaatggaaa cgaaatatta aaaaactttc catgtgtgaa accccgtccc
    27661 gagtctcaga aactccccgg taaagctggc agtctaatat agcgaaggaa gcagtcgtcg
    27721 gccttttggt ggtatgaccc ttaaaaaaca tggttcaggg gctgtggcgg atccgacggt
    27781 ggtagcttga tcccgttcag gaaggtgatt tctccgtcgt ctcctccttc gttcattaaa
    27841 aatgagcaag gagatttata tgaaaaaatc ctcccgaatg tctattgtcc attctcatgc
    27901 tgcgggcgtt gatattgggg ccaaatttca cgttgtggcc gtaccgcctg atgctgacgc
    27961 cgagccaata aggacttttc aaagtttcac cggcgacctt catcgtatgt ctgactggct
    28021 caaaacctgt catattacaa ccgtcgccat ggagtccacc ggcgtttact ggttgcctgc
    28081 ttttgagatc cttgaagcag cgggttttaa tatgatactc gttaatgtca gtgccgcaaa
    28141 aaatgttccc ggtcgcaaga ctgatgtcaa cgacgcacag tggttgcaga gacgacatgc
    28201 atttggtctt ctccgggcca gtttcagacc tgtacacgac atttcagtcc tcaggtctta
    28261 tctgaggcaa cgagaacgct tgactgaata ccgggctgcg catattcagc atatgcagaa
    28321 agcgctgatg cagatgaatg tgcaattgca tcatgccgta tcggatatca ctggcgtcac
    28381 cggtctctcg atcgtcagag ctattgtcag cggtgagccc gatccttcag tattgatcca
    28441 gtaccgtgat gtgagatgta agaagacgcc agaagtactt cagcaggcac tgacagggaa
    28501 ctggcaacct gagcatcttt ttgcacttga gcagtcggtg gcatttttct atttttacca
    28561 ggaaaagatc cgtgaacgtg acgatcaaat cgaaattcat taatacaact cagcaccggg
    28621 actgaaaatc ctgaaggagt attaccctgc gccagacatc gtacaaagca acccaatcag
    28681 ctttccttga tgtcagaccg ttactctgga aaattaccac ggcagatctg acacaaatac
    28741 atggggttgg gccttatctg gcactaaaat tcgttgccga acgcggtact gacatgaatc
    28801 gctggcctga tgcgagccat tttacctcgt ggctattttg tccacgggaa ataagatctc
    28861 cggtggaaaa gtgttgtcct cgaaaacgag acgttcatcc agttgaattg ctgctgcact
    28921 caggctggca gccacaacga ttggtcggag cgatactgcg ttgggtgctt tttatcgtcg
    28981 attatcatcc cgtataggta aagcaaaagc agtcactgca acggcaagaa aactggcaac
    29041 actgttctac aatgccttga aatatggtaa aaaatatgtc gacaattgtg ctgattacta
    29101 tgaagagcgt taccgtagtt gcgtactcaa tggacttaaa cgaagagtaa aatcactggg
    29161 ttattctctc cagcaagacc ctgaattatg agagtgaacc aggagtttct caggaaggct
    29221 accgcgctct tgatgtcaga ttcctgaaca gtctcaataa tcgggaaact cagagcgcat
    29281 tatcctgtgt tcacactctg tcatgtgttc gcagttcatc gctgcagcta cagatattgg
    29341 aaaacccgtc ctgaaaaaac agacggcaga tgggctaggt aattgacttg catggcggag
    29401 ctaggagcat tgccacaatg gcaacccgga gagattacca gatgggacgc tggcttgctg
    29461 gcaggctcat gaaaaaacag gggctggtca actgtcagcc gccgccccac cggtcatgaa
    29521 catgtcgcta ttcctaacta tcttgaaccg cacttcgctg tgacacagct aaatcaagtg
    29581 tgatgacgtg acgtatatct ggataggtaa acgatgggga tacctcgcag tagtacttgc
    29641 tctgttcgca ataaagcccg gggatgggca atgtcgttct caccggacag cagactgact
    29701 ataaaagcgc tgaagatggc cgaggaaacg cgaggtaaac cagagggagt tatgttcctt
    29761 agcaacagga gagccattac acaagtagac agttcaggca ggtgatgtgg cgttatcgga
    29821 tcaaggagag tatgagtcgt cggggaaact gctgggatat tagcctaatg gtacgtttct
    29881 tcagaagtct gaagaacgag tgggcaccag tgactggtta ctttagcctt aacgaaggtg
    29941 tccatacaat aacagagtat ataattgaat attaagtgca ctccggcctc atgaatataa
    30001 cggtggatta cctccgaacg aatcggaaaa tagatactgg aaaaaatctg aaattgtgac
    30061 cattttttgt tgaccactac aaataaataa aagagttgca gtgcttgctc ggtaagaaaa
    30121 caatggaagt tgagatcctc aaagaagctg tggagtacac cactctacag tagttgcgca
    30181 aatttggagt ggacgataat gtgatcgtct ataagggcaa cgctatcata gtcttgtcct
    30241 ggcgggtaaa aaagcgcgct taccttaacg ataagcgcgc cgctgttcag gccttgagtg
    30301 gttattcaat tcctgtggtg actgtaaaag tgcgcgtttg ctgcggtgca acctgaatca
    30361 gcgtgccatt acgttgcgcg gcaagatacc cctcaggccg acaggttgca ggtaatgcaa
    30421 aggcggctac ctgttgctct ccgttataaa ggatccagcg tgtcacataa tttagttcag
    30481 cactgtagaa acgagtaaca aacgtagtgc catcgggaga gatcatgcga aactctggct
    30541 gatctgtata agcgtccagt ttgtctgcaa agaagacaat ttctggatca taaaattccg
    30601 gttgactcag cgtcgacaga gaggcttctc cctgcataat ccgttgatta aacgccagcc
    30661 actgagcggt gggattaaca tgcgaaggca ctgattcacg taatcttaat atttcgtccg
    30721 ggatattctg gctgaatgta gcatttggta tatatgcata attcatgtgg cacatatatt
    30781 gtagtggcat atctacagaa gccagattgg ttacggccat cttaatatcg aacagtgtag
    30841 aggatttgtg aaggaccact gttggttgag ccagataatg atgaccgaaa cccattacat
    30901 actcgtaacg cccgttaagg cgtaacatat ctccgtctaa ttccagccat gcttcatcca
    30961 tcgcggcaca ggccatttca ccgtgtagca gatgagtatc ttccgcagat gggcagccat
    31021 tagccagcaa acctgaatga aaagcaaaac agccataggt ctctatcacc tctgtcgccg
    31081 gtttaggctg gcgaaacata ttgcacatgg tgaggccgtg tccatcaaat tgcgcatccc
    31141 aaatcatctg ccccatccag ggaagaataa tcaaatgtcc acgactgttt gcaattttaa
    31201 gcccctcgac accgctgtca tagcgaaaag acgtgacagt aaaatcacta ttttccagca
    31261 agatacgagg tttctcgcca aaaagcgccc gccacaaatt aatacgcgta ctcataacgg
    31321 ttctcctcag gacgctgtga cttcagccag tgcggtacgt actttgcttt cacgccagaa
    31381 gtagactccg acatagacaa agcagagcat agaaaccagg aatgaaagct gtagtgagtg
    31441 gaacatatct gcaatatatc cctgaattgc cggaaccacc gcggcaccga caatagccat
    31501 aacaatgact gctcctgcca tttctgtatg ttcgttatca actgtatcca gtgttcctgc
    31561 atagatcgtc gcccagcaag ggccaaacaa aacacttacc aggacggcga catagaccgc
    31621 gctgaaactt ggagccagtg caacatatgc caggaacagc gcccctataa cggaatagag
    31681 aatcaatact ttttccggat taaaacgcgt cataaggatg ttggctataa acttgccaat
    31741 aaagaagcag gcaaagctat agaccatgaa gtttgaagca tcacgttcgt tgatatcgcc
    31801 caactccagc gccagacgga tggtaaatga ccatactgcg acctgcatac ccacataaag
    31861 gaactgcgcc acaataccgc gacgaaagcg cggatttcta gccagatagc gcagcgtatc
    31921 cattgctgac gggcgtttat agtgacttgt ctgtgccact ttacaggttg ggaagcgggt
    31981 taaaaggaac aacaccatga ccacaaccag aatcataatc atatacttat acggttcaag
    32041 ggtgttctct aacattagca ccttaaagtt gtgaatttgc tcggcgttca ttccggacat
    32101 ctgcttctca aggctttccc cctcggagaa aaccagatat ttgcccaata aaataccaga
    32161 cgcagcacca atcggataaa aggtctggct gatattgagc cgcaatgtgg cataggcttt
    32221 tggaccgatc attgaactgt atgtgttcgc tgcagtttca aggaaactca ggccaatcgc
    32281 aatcgcaaaa atagctgcaa ggaacatagt gtaggttgcc atatgcgagg cagggaaaaa
    32341 aagtgtacaa ccaccaatat acagcgtcag gccaattaaa attgccacct tataactggt
    32401 ctttttaatc acaagggatg ctggtattgc aattaaaaaa taacctccat aaaatgcgct
    32461 ctgcaccaat gctgaagcaa agttacttag ggaaggtgcg aataagcggg gaaattcttc
    32521 tcggctgact cagtcatttc atttcttcat gtttgagccg attttttctc ccgtaaatgc
    32581 cttgaatcag cctatttaga ccgtttcttc gccatttaag gcgttatccc cagtttttag
    32641 tgagatctct cccactgacg tatcatttgg tccgcccgaa acaggttggc cagcgtgaat
    32701 aacatcgcca gttggttatc gtttttcagc aaccccttgt atctggcttt cacgaagccg
    32761 aactgtcgct tgatgatgcg aaatgggtgc tccaccttgg cccggatgct ggctttcatg
    32821 tattcgatgt tgatggccgt tttgctcttg cgtggatgct gtttcaaggt tcttaccttg
    32881 ccggggcgct cggcgatcag ccagtccaca tccacctcgg ccagctcctc gcgctgtggc
    32941 gccccttggt agccggcatc ggctgagaca aattgctcct ctccatgcag cagattaccc
    33001 agctgattga ggtcatgctc gttggccgcg gtggtgacta ggctgtgggt caggccactc
    33061 ttggcatcga caccaatgtg ggccttcatg ccaaagtgcc actgattgcc tttcttggtc
    33121 tgatgcatct ccggatcgcg ttgctgctct ttgttcttgg tcgagctggg tgcctcaatg
    33181 atggtggcat cgaccaaggt gccttgagtc atcatgacgc ctgcttcggc cagccagcga
    33241 ttgatggtct tgaacaattg gcgggccagt tgatgctgct ccagcaggtg gcggaaattc
    33301 atgatggtgg tgcggtcagg caaggcgcta tccagggata accgggcaaa cagacgcatg
    33361 gaggcgattt cgtacagagc atcttccatc gcgccatcgc tcaggttgta ccaatgctgc
    33421 atgcagtgaa tgcgtagcat ggtttccagc ggataaggtc gccggccatt accagccttg
    33481 gggtaaaacg gctcgatgac ttccaccatg ttttgccatg gcagaatctg ctccatgcgg
    33541 gacaagaaaa tctcttttct ggtctgacgg cgcttactgc tgaattcact gtcggcgaag
    33601 gtaagttgat gactcatgat gaaccctgtt ccatggctcc agatgacaaa catgatctca
    33661 tatcagggac ttgttcgcac cttccatcag agaccaggga tatcgtggcg gaatgaccat
    33721 tctcagggcg ttcattcgtt ctctctcggt ttctcaggag caggagcctg ccgttcggtt
    33781 cgaaactgaa cccggacgac agatgcaggt tgactggggc actatgcgta atggtcgctc
    33841 accgcttcac gtgttcgttg ctgttcccgg atacagccga atg
//
LOCUS       DAZGJT010000044        33273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZGJT010000044 DAZGJT010000000
VERSION     DAZGJT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.044"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /gene="pspE"
                     /locus_tag="ACHKFK_002319"
     CDS             complement(<1..242)
                     /gene="pspE"
                     /locus_tag="ACHKFK_002319"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO9052647.1"
                     /translation="MFKKGLLALTLVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEIL"
     gene            complement(317..538)
                     /gene="pspD"
                     /locus_tag="ACHKFK_002320"
     CDS             complement(317..538)
                     /gene="pspD"
                     /locus_tag="ACHKFK_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO9052648.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(547..906)
                     /gene="pspC"
                     /locus_tag="ACHKFK_002321"
     CDS             complement(547..906)
                     /gene="pspC"
                     /locus_tag="ACHKFK_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO9052649.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(906..1130)
                     /gene="pspB"
                     /locus_tag="ACHKFK_002322"
     CDS             complement(906..1130)
                     /gene="pspB"
                     /locus_tag="ACHKFK_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO9052650.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1184..1852)
                     /gene="pspA"
                     /locus_tag="ACHKFK_002323"
     CDS             complement(1184..1852)
                     /gene="pspA"
                     /locus_tag="ACHKFK_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO9052651.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKDREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2019..2996
                     /gene="pspF"
                     /locus_tag="ACHKFK_002324"
     CDS             2019..2996
                     /gene="pspF"
                     /locus_tag="ACHKFK_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO9052652.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPMLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTEHARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPSEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQVSLEQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            3152..3289
                     /locus_tag="ACHKFK_002325"
                     /pseudo
     CDS             3152..3289
                     /locus_tag="ACHKFK_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Putrescine importer PuuP"
     gene            3423..3668
                     /gene="ymjA"
                     /locus_tag="ACHKFK_002326"
     CDS             3423..3668
                     /gene="ymjA"
                     /locus_tag="ACHKFK_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2543 family protein"
                     /protein_id="HFO9052653.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            3981..5624
                     /gene="sapA"
                     /locus_tag="ACHKFK_002327"
     CDS             3981..5624
                     /gene="sapA"
                     /locus_tag="ACHKFK_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO9052654.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYAGKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQYIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            5621..6586
                     /gene="sapB"
                     /locus_tag="ACHKFK_002328"
     CDS             5621..6586
                     /gene="sapB"
                     /locus_tag="ACHKFK_002328"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO9052655.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEIVMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            6573..7463
                     /gene="sapC"
                     /locus_tag="ACHKFK_002329"
     CDS             6573..7463
                     /gene="sapC"
                     /locus_tag="ACHKFK_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO9052656.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHSLDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            7463..8455
                     /gene="sapD"
                     /locus_tag="ACHKFK_002330"
     CDS             7463..8455
                     /gene="sapD"
                     /locus_tag="ACHKFK_002330"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO9052657.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            8457..9263
                     /gene="sapF"
                     /locus_tag="ACHKFK_002331"
     CDS             8457..9263
                     /gene="sapF"
                     /locus_tag="ACHKFK_002331"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO9052658.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            complement(9311..10474)
                     /locus_tag="ACHKFK_002332"
     CDS             complement(9311..10474)
                     /locus_tag="ACHKFK_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFO9052659.1"
                     /translation="MARVSLSWALILGLLAGIGPMCTDLYLPALPEMSEQLAATTTIT
                     QLTLTASLIGLGVGQLLFGPLSDKIGRKRPLILSLLLFIVSSILCATTNNIYWLVVWR
                     FIQGIAGAGGSVLSRSIARDKYQGVTLTQFFALLMTVNGLAPVLSPVLGGYIVSTFDW
                     RTLFWVMAEISTVLLLGCLLFINETLPENKRGSSLLLTGRSVVQNRRFMRFCLIQSFM
                     LAGLFAYIGSSSFVLQKEFGFSPMQFSLVFGLNGIGLIIASWIFSRLARRINAMTLLR
                     GGLIAAILCALLTVLCAWVQLPIPALVALFFTIAFCSGIGTVGGAEAMSAVGTQESGT
                     ASALMGMSMFVFGGIAAPLSGIGGETLLKMSLAITVCYTLALLVALTRIDNQK"
     gene            complement(10483..11856)
                     /locus_tag="ACHKFK_002333"
     CDS             complement(10483..11856)
                     /locus_tag="ACHKFK_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HFO9052660.1"
                     /translation="MLRRSLIFLVLLSAGCVSLDPHYSTPESPIPATLPGAQGQGKAI
                     SHDWQQVIHDPRLQQVVTIALNSNRDVQKAIADIDSARALYGQTNASLFPTVNAALSS
                     TRSRSLANGTGTTAEADGTVSSYTLDLFGRNQSLSRAARETWLASEFTAQNTRLTLIA
                     EISTAWLTLAADNSNLALAKETMASAENSLKIIQRQQQVGTAAATDVSEAMSVYQQAR
                     ASVASYQTQVMQDKNALNLLAGTTLAENLLPGTLESLPEQMISLVPAGVSSDVLLRRP
                     DIQEAEHNLKSANADIGAARANFFPTISLTASAGVGSDALSSLFSHGMQIWSFAPSVT
                     LPLFTGGSNLAQLRYAEAQKRGLIATYEKTVQSAFKDVANALARRTTLEEQLDAQRQY
                     VKAEQQTVDVGLRRYQAGVGDYLTVLTAQRSLWSAQQELLALQLTDFTNRITLWQSLG
                     GGMSSLK"
     gene            complement(11860..14967)
                     /locus_tag="ACHKFK_002334"
     CDS             complement(11860..14967)
                     /locus_tag="ACHKFK_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HFO9052661.1"
                     /translation="MFSRFFVRRPVFAWVIAILIMLAGILAIRTLPVAQYPDVAPPTI
                     KISATYTGASAETLENSVTQVIEQQLTGLDNLLYFSSTSSSDGSVSINVTFEQGTDPD
                     TAQVQVQNKIQQAESRLPSEVQQTGVTVEKSQSNFLLIAAVYDTTDKASSSDIADWLV
                     SNVQDPLARVEGVGSLQVFGAEYAMRIWLDPAKLASYSLMPSDVQSAIEAQNVQVTAG
                     KIGALPSPDTQQLTATVRAQSRLQTVDQFKNIIVKSQSDGAVVRIKDVARVEMGSEDY
                     TAIGKLNGHPSAGVAVMLSPGANALNTATLVKDKIAEFQRNMPQGYDIAYPKDSTEFI
                     KISVEDVIQTLFEAIVLVVCVMYLFLQNLRATLIPALAVPVVLLGTFGVLALFGYSIN
                     TLTLFAMVLAIGLLVDDAIVVVENVERIMRDEGLPAREATEKSMGEISGALVAIALVL
                     SAVFLPMAFFGGSTGVIYRQFSITIISAMLLSVVVALTLTPALCGSVLQHVPPHKKGF
                     FGAFNRFYRRTEDKYQRGVIYVLRRTARTMGLYVVLGGGMALMMWKLPGSFLPTEDQG
                     EIMVQYTLPAGATAARTAEVNRQIVDWFLINEKANTDVIFTVDGFSFSGSGQNTGMAF
                     VSLKNWSQRKGAENTAQAIALRATKELGTIRDATVFAMTPPAVDGLGQSNGFTFELLA
                     NGGTDRETLLQMRNQLIEKANQSPELHSVRANDLPQMPQLQVDIDSNKAVSLGLSLND
                     VTDTLSSAWGGTYVNDFIDRGRVKKVYIQGDSEFRSAPSDLGKWFVRGSDNAMTPFSA
                     FATTRWLYGPERLVRYNGSAAYEIQGENATGFSSGDAMTKMEELANSLPAGTTWAWSG
                     LSLQEKLASGQALSLYAVSILVVFLCLAALYESWSVPFSVILVIPLGLLGAALAAWMR
                     DLNNDVYFQVALLTTIGLSSKNAILIVEFAEAAVAEGYSLSRAALRAAQTRLRPIIMT
                     SLAFIAGVMPLAIATGAGANSRIAIGTGIIGGTLTATLLAIFFVPLFFVLVKRLFAGK
                     PRRQE"
     gene            complement(14967..16083)
                     /locus_tag="ACHKFK_002335"
                     /pseudo
     CDS             complement(14967..16083)
                     /locus_tag="ACHKFK_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(16232..16798)
                     /locus_tag="ACHKFK_002336"
     CDS             complement(16232..16798)
                     /locus_tag="ACHKFK_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO9052662.1"
                     /translation="MTSKLEIRHKQRQDEIINAARRCFRRCGFHAASMSQIASEAQLS
                     VGQIYRYFANKDAIIEEMVRRIIDFRIAQMDIDARTDHFPEVLALRKSLNEDDDALML
                     EVAAEATRNPRVMAMLEEADARMFANGCAHMKRMHPHLSDEHIRCCVEVFATMMEGTV
                     YRRLTPQKSDPQHLQEIYQDIVSMLINK"
     gene            17276..18064
                     /gene="fabI"
                     /locus_tag="ACHKFK_002337"
     CDS             17276..18064
                     /gene="fabI"
                     /locus_tag="ACHKFK_002337"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO9052663.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDTSIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            18209..19336
                     /gene="yciW"
                     /locus_tag="ACHKFK_002338"
     CDS             18209..19336
                     /gene="yciW"
                     /locus_tag="ACHKFK_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO9052664.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVCDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNTQPDNQLAALLCARIN
                     GSPACFATCMDSSNEYKKISALLRKGENEINQWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            19404..21338
                     /gene="rnb"
                     /locus_tag="ACHKFK_002339"
     CDS             19404..21338
                     /gene="rnb"
                     /locus_tag="ACHKFK_002339"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO9052665.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DGKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSTDGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETAYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(21415..21519)
                     /locus_tag="ACHKFK_002340"
     CDS             complement(21415..21519)
                     /locus_tag="ACHKFK_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052666.1"
                     /translation="MTYMKLTAAKHIVFFIYLFVIQFWKNASFQTKVS"
     gene            21573..23558
                     /gene="pdeR"
                     /locus_tag="ACHKFK_002341"
     CDS             21573..23558
                     /gene="pdeR"
                     /locus_tag="ACHKFK_002341"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO9052667.1"
                     /translation="MKTVRESATLYNFLGSHNPYWRLTESSDVLRFSTTEITEPDRIL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWGGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQELIDHAINQADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEALA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSAAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSRLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(23540..23668)
                     /gene="ymiD"
                     /locus_tag="ACHKFK_002342"
     CDS             complement(23540..23668)
                     /gene="ymiD"
                     /locus_tag="ACHKFK_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO9052668.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTEYSAKYIYSSTYARFR"
     gene            23706..23879
                     /gene="yciZ"
                     /locus_tag="ACHKFK_002343"
     CDS             23706..23879
                     /gene="yciZ"
                     /locus_tag="ACHKFK_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO9052669.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            23969..24718
                     /gene="yciT"
                     /locus_tag="ACHKFK_002344"
     CDS             23969..24718
                     /gene="yciT"
                     /locus_tag="ACHKFK_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO9052670.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEQSKLTVHVVDI"
     gene            24987..25205
                     /gene="osmB"
                     /locus_tag="ACHKFK_002345"
     CDS             24987..25205
                     /gene="osmB"
                     /locus_tag="ACHKFK_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO9052671.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(25331..25657)
                     /gene="yciH"
                     /locus_tag="ACHKFK_002346"
     CDS             complement(25331..25657)
                     /gene="yciH"
                     /locus_tag="ACHKFK_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO9052672.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVALDDAELTKLAAELKKKCGCGGAVKDGIIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(25657..26394)
                     /gene="pyrF"
                     /locus_tag="ACHKFK_002347"
     CDS             complement(25657..26394)
                     /gene="pyrF"
                     /locus_tag="ACHKFK_002347"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO9052673.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAAMAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(26586..27755)
                     /gene="lapB"
                     /locus_tag="ACHKFK_002348"
     CDS             complement(26586..27755)
                     /gene="lapB"
                     /locus_tag="ACHKFK_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO9052674.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(27762..28070)
                     /gene="lapA"
                     /locus_tag="ACHKFK_002349"
     CDS             complement(27762..28070)
                     /gene="lapA"
                     /locus_tag="ACHKFK_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO9052675.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVAPHSSASKE
                     "
     gene            complement(28219..28983)
                     /gene="pgpB"
                     /locus_tag="ACHKFK_002350"
     CDS             complement(28219..28983)
                     /gene="pgpB"
                     /locus_tag="ACHKFK_002350"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO9052676.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWLSGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAIMLFAILAAAILMGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            29153..29743
                     /gene="ribA"
                     /locus_tag="ACHKFK_002351"
     CDS             29153..29743
                     /gene="ribA"
                     /locus_tag="ACHKFK_002351"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO9052677.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIIERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(29807..32475)
                     /gene="acnA"
                     /locus_tag="ACHKFK_002352"
                     /pseudo
     CDS             complement(29807..32475)
                     /gene="acnA"
                     /locus_tag="ACHKFK_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(32625..32720)
                     /gene="ymiC"
                     /locus_tag="ACHKFK_002353"
     CDS             complement(32625..32720)
                     /gene="ymiC"
                     /locus_tag="ACHKFK_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO9052678.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFCAELLWIITH"
     gene            complement(32834..33001)
                     /gene="yciX"
                     /locus_tag="ACHKFK_002354"
     CDS             complement(32834..33001)
                     /gene="yciX"
                     /locus_tag="ACHKFK_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO9052679.1"
                     /translation="MVGQEQLESSPLCQHSDNEPEAKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(33004..33144)
                     /gene="ymiA"
                     /locus_tag="ACHKFK_002355"
     CDS             complement(33004..33144)
                     /gene="ymiA"
                     /locus_tag="ACHKFK_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO9052680.1"
                     /translation="MRLAMPSGNQEPRRNPELKRKAWLAVFLVSALFWVVVALFIWKL
                     WG"
BASE COUNT         8202 a         8681 c         8069 g         8321 t
ORIGIN      
        1 ctaaggatct cttttgcctg ccctgactgg cgtccggcat tgcaatacac tttcacggtg
       61 tcgtttttat ccggaacggc ggtggcaatg cgctctttca cttccttcaa gggaatattg
      121 atggccccct gaacgtgttc ttgctgatac tgctctggaa cacgaacatc gatccagtgt
      181 tcagcggcga aaacgggcag tgaaaacacc agtgtcagag caagtaagcc ttttttaaac
      241 atgtaagcgt ccttctatgg atgaaaattg ctatccatta tacgcctgag atttaaccat
      301 ttttgcgaaa ctcccctcac cttttataac gctgcgccag tttattggca gcccgactta
      361 acagcggttc cagcgccaca gccagcaaca ttttcagcgg tcggcgagca actgatttta
      421 tcgcccagcc cgccacaccc gccgggccat agcgcagtgc ggtaagaagt accagcttgc
      481 ctgctaattt gaacccaggc ttcacctttt gcccggcctg ttgccagcga gtattcataa
      541 ctttcctcac agttgacgga aacggctacg taacgtgaaa gtatcggaag tgacataacg
      601 ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt catccagcaa
      661 ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg tccggcattg gatcaagcgc
      721 aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaga aaatcgacag
      781 caccaccagg atacgcacca gttttactgg tacatcaaaa tagttggcaa tcccggcgca
      841 gacgccacgg accatgccct gctgcggaat acgccataat tttttattga gattaatgcc
      901 cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatagattc cagcgcctga
      961 atacgttcgc gcatccgttt tgcttcatca gccagttgcg ctaatcgctg ctgctcactt
     1021 tgcgacaatt cactgcgacc agaacgattg ctgtaatgca gccataacca gatcggtaaa
     1081 acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat aaggactcct
     1141 tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattattga ttgtcttgct
     1201 tcattttggc ttttaattgt gccagttgct cgctgattgc atcatcggct ttcagttcgg
     1261 caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct tccgcttcca
     1321 tctggtcaat tcgacgttcg aaagattcga aacgagccat tgcttcatcc agtttgccac
     1381 tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ctgatggcgt aacatcaatg
     1441 cctgctggcg agcgcgtgtt tcactcaatt tgttttccag ctcgccgatc tctttcttca
     1501 tgcgtgccag cgtatcgtcc accagcgtca cttcatgttc caggctctta atcagatcgg
     1561 tcagtttctg tttttcaatt aacgccgcac gcgccaggtc ttctctatct ttcagcagtg
     1621 ccagttcggc tttttcctgc cattcaactt cacgcgccga cgcttgttca atacggcgag
     1681 tcagctgttt cttttctgcc agcgcacgcg ccgaagtaga acgtacttca accagtgtat
     1741 cttccatctc ctggatcatc agacgcacca gtttctgtgg atcttccgct ttctctaaca
     1801 gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc ataatgttgt
     1861 cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa
     1921 ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat
     1981 gaattagcga attacactaa caagtggcga atttcatcat ggcagaatac aaagataatt
     2041 tacttggtga ggcgaatagc tttctcgaag tgctggaaca ggtttcgcat ctcgcaccac
     2101 tggataaacc gatgctcatc atcggcgaac gcggcaccgg taaagagttg attgccagcc
     2161 gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt
     2221 taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg tttaccggtg
     2281 cgcaaaaacg tcatcctggg agatttgaac gtgccgatgg cggtacgcta tttcttgatg
     2341 aactcgctac ggcaccgatg atggtgcagg aaaaattatt gcgtgtgatt gagtacggtg
     2401 aactggagcg cgttggcggt agccagccat tgcaggtgaa tgtgcggttg gtatgcgcga
     2461 cgaatgccga tctccctgcg atggtcaatg aaggtacttt tcgcgctgac ctgctcgacc
     2521 gactagcgtt cgatgttgtg caactgccgc cactacgcga acgtgaaagc gacattatgt
     2581 taatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct ctgttcccgg
     2641 gttttaccga gcacgccaga gaaacattac tgaattatcg ctggccggga aatatacgtg
     2701 aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat tatccgcttg
     2761 atgacatcat tattgatccc tttaaacgcc gtccatctga agaagccatc gccgtttcag
     2821 aaaacacttc gcttccgacg ctgccactgg atttacgcga gtttcagatg cagcaggaaa
     2881 aagaactgct tcaggtcagc ttggaacaag ggaagtataa ccagaagcgc gccgctgaat
     2941 tgctggggtt aacctatcat cagttccgcg cgttgttgaa aaagcaccag atttagcgaa
     3001 gttgggcaat acaacttgcc ggatgcagcg tgaacgcttt atccggcata cataatcaat
     3061 acgtcgtttg caggcctgat aagcgctgcg catcaggcat tattgcgtta accctgcttc
     3121 tgcgtattaa tctctcagcg catcctgcaa aatttgcatt gccgcatcga attgcgcaac
     3181 cgggatggta tgggcttcgt taggcggcgc atatttgtgg tatttgatcc gccgctatcg
     3241 caaagtgccg ttgtatgatg gcgacagaac gccggtgagc gaaacgtaat agtcgaatgc
     3301 gcggcgcatt atcctgcctg agggtaatgc accgcgccat taactataaa aatatgtatg
     3361 acccggatca cgagtgctat catccgcctt tatgcttagc gcattaaccc caggggcaaa
     3421 taatgaacca cgatatcccg ctaaaatatt ttgatattgc cgatgagtac gcgaccgaat
     3481 gcgcagagcc tgtcgcagac gctgaacgca caccactggc ccactacttc cagttactgc
     3541 tcacccgttt gatgaacaat gaagagatca gtgaagaagc ccaacatgaa atggctgctg
     3601 aagcggggat taatcccgta cgcattgatg agatcgcaga gtttttgaat caatggggta
     3661 atgagtaatt atttaattgc agacgatcgg tcagtatcgt tgcccggata aagcgttcgc
     3721 gccgcatccg gctaaaaatg acacgcacag cagcgtgctt taatgggttt ctcacgatcg
     3781 taccgctctg ctcctagcta agttgttctg tgagttatag ttataaagaa atcacgggta
     3841 cgaatgttga actgatggcg acgccacctg ttcaatgacc aaccacacgg ccctcattac
     3901 gacctctgaa cacaaacggt aatggcaaat gtcattaaat attagtaaga tagcctcttt
     3961 acataaaatc ccttaatatt atgcgccagg tattatcgtc tcttttggtg attgctggac
     4021 ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc gcatgctgat atccgcgaca
     4081 gcggttttgt ctattgcgtc agcgggcaag tcaacacctt taacccatcc aaagcgagca
     4141 gtgggttaat tgtcgatacc cttgccgccc agttttatga tcgactgctg gatgtcgatc
     4201 cctataccta tcgcctgatg ccggaacttg ccgaaagctg ggaagtactc gacaacggcg
     4261 cgacctatcg cttccacctg cgtcgcgatg ttccgtttca aaaaaccgac tggtttactc
     4321 ccactcgtaa aatgaatgcc gatgatgtgg tgtttacctt ccaacggatt tttgaccgca
     4381 acaacccgtg gcataacgtc aacggcagta acttccccta tttcgacagt ctgcaatttg
     4441 ccgataacgt gaaaagcgtc cgcaagctgg ataatcatac cgttgagttc cgtctcgccc
     4501 agccggatgc ttcttttttg tggcacctgg cgacccatta tgcttcggtc atgtcggcag
     4561 aatatgcagg gaagttagag aaagaagatc gccaggagca actcgaccgt caaccggtcg
     4621 gcaccggtcc gtatcagttg tcggaatacc gcgccgggca atatattcgc ttacaacgtc
     4681 atgatgattt ctggcgtggt aagccgttga tgccacaagt ggtggtagat ttaggctctg
     4741 gcggtacagg acgattgtcg aaactcctga ccggggagtg cgacgttctg gcctggcctg
     4801 ctgccagtca gctatccatt ttgcgtgacg acccacgctt gcgtttaacg ctgcgtcctg
     4861 ggatgaatgt cgcctatctg gcattcaaca ccgccaaacc gccgctaaat aatccggcgg
     4921 tccgccatgc gctggcactg gcgattaata accagcgcct gatgcaatcc atctattatg
     4981 gtacggctga aacggctgcc tctattttac cgcgagcctc gtgggcctat gacaacgagg
     5041 ctaaaattac tgaatacaat ccggcaaaat cgcgcgaaca gttgaaggcg ttggggctgg
     5101 aaaacttaac gctgaaactg tgggtgccca cacgttcgca ggcgtggaac cccagtccac
     5161 tgaaaaccgc cgaactgatt caggcggata tggcacaggt tggcgtaaaa gtggtgattg
     5221 tgccggtaga aggtcgtttt caggaggcgc ggttgatgga tatgagccat gatctgacgt
     5281 tatccggttg ggcgacggac agtaacgacc cggacagttt cttccgtccg ttactgagct
     5341 gcgcagcaat tcattcgcag accaacctcg cccactggtg cgatccgaaa ttcgacagcg
     5401 tgttgcgtaa ggcgctctct tcgcagcagc tggcggcgcg tattgaagcc tatgacgaag
     5461 cgcaaagtat tctggcgcag gaattaccta ttctgccgct ggcgtcgtca ttgcgtttgc
     5521 aggcctatcg gtacgatatc aaaggtctgg tacttagccc gtttggtaac gcctcctttg
     5581 ctggggtgta tcgcgagaaa caggatgagg tgaaaaagcc atgattatct tcaccttacg
     5641 ccgtattttg ttattgattg tgaccttgtt cctgctgacc ttcgttggct tcagcttgag
     5701 ctatttcacc ccacatgcgc cgttacaagg cgcgtcactg tggaatgcct gggtgttctg
     5761 gtttaacggc ctgatccact gggattttgg tgtttccagc attaacggtc aaccgattgc
     5821 cgaacagtta aaagaggtct tcccggcgac gatggaactg tgcatcctcg ccttcggttt
     5881 tgcgctgatt gttggcatcc ccgtaggaat gattgccggg attacgcgcc ataagtggca
     5941 ggataacttg atcaacgcca tcgccttact gggcttttca atcccggtgt tctggctggc
     6001 gcttctgctg acgctgtttt gttcactcac gctaggttgg ttgcccgttt ctgggcgttt
     6061 cgatttgctc tacgaagtga aaccgattac cggttttgcg ttgattgatg cctggctttc
     6121 ggactcaccg tggcgggatg aaatagtcat gagcgcaatc cgccatatga tattgcccgt
     6181 gatcaccctg tcggttgcgc caacaactga agtgatccgg ctaatgcgta tcagcactat
     6241 cgaagtgtat gaccagaatt atgtaaaagc agcggcgacc cgcggtttgt cgcgctttac
     6301 tattttgcgt cgccacgttc tgcataacgc gttacctccg gttattcctc gcctgggctt
     6361 acagttttct accatgctga ctctggcgat gatcaccgaa atggttttta gctggccggg
     6421 tttagggcgc tggttaatta acgccatccg ccagcaggat tacgcagcca tttccgccgg
     6481 agtgatggtg tgtggctcac tggtcattat tgttaacgtg atttctgata ttttgggtgc
     6541 catggctaac cctctgaaac ataaggaatg gtatgcctta cgatagcgta tacagcgaaa
     6601 agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt ttatagtgac gcctctgcga
     6661 tggtcggcct gtacggctgc gcggggctgg ctgtactgtg tatttttggc ggctggtttg
     6721 cgccttacgg catcgaccag caatttctcg gttatcaatt attgccgccg tcatggtcgc
     6781 gctatggcga agtttctttc tttctgggga cggacgacct cggacgcgat gttttaagcc
     6841 gtttactgag cggagctgcg ccaaccgtag gtggcgcatt tgtggtgacg cttgccgcga
     6901 cgatctgtgg cctggtactg ggcaccttcg ccggggcgac ccacggccta cgctcagcgg
     6961 tgcttaacca tagtctggat accttactgg cgatcccttc gctgctgctg gcaattatcg
     7021 ttgtagcgtt tgccggaccg agtttgtctc acgccatgtt tgctgtctgg ctggcattgc
     7081 tgccgcgtat ggtacgttcg atttacagca tggtgcatga cgaactggaa aaagagtacg
     7141 ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat tctctggttt gctgtgatgc
     7201 ccaacatcac tgccggactg gtcaccgaga tcactcgcgc attgtcgatg gcaattctcg
     7261 atatcgccgc gctgggcttt cttgacctcg gcgcacaact gccctcgcct gaatggggcg
     7321 cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc atggactgtc atgctgccag
     7381 gtgcggcaat tatgattagc gtgctgttgg ttaacctcct tggcgacggt gtccgtcgtg
     7441 caattattgc gggggtggaa taatgccatt actcgatatt cgtaacctga ccattgaatt
     7501 taaaaccggt gatgagtggg ttaaagccgt cgaccgcgta agtatgacct taaccgaagg
     7561 tgaaatccgc ggtcttgttg gcgaatcagg ttccggcaaa agtttgattg cgaaagcgat
     7621 ttgtggggtg aataaagata actggcgtgt tactgctgac cgtatgcgtt ttgatgatat
     7681 cgatttgctg cgtctctccg cacgcgaacg gcgcaagctg gttggtcaca acgtgtcgat
     7741 gattttccag gaaccgcagt cgtgtcttga cccttcagaa cgtgtgggtc gccagttgat
     7801 gcaaaacatc cctgcctgga cctataaagg ccgttggtgg cagcgttttg gctggcgcaa
     7861 acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa gatcacaaag atgcgatgcg
     7921 cagttttccc tatgagttga ccgaaggtga atgtcagaaa gtgatgatag ccattgcact
     7981 ggcgaatcaa ccgcgtctgc taattgctga cgaaccgacc aactcaatgg agccaacaac
     8041 ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa aacagcaata ccactatttt
     8101 gcttatcagc catgacttac aaatgcttag ccaatgggcg gataaaatta acgtgcttta
     8161 ctgcggtcaa acggtggaaa ccgcgccgag taaggagttg gtgacgatgc cacaccatcc
     8221 ttatacccag gcgctgatcc gcgcgatacc agacttcggc agcgcgatgc cgcataaaag
     8281 tcgcctcaat acgctgcccg gcgctatccc gctgctggaa cagttaccga ttggttgtcg
     8341 tctggggcca cgttgcccgt atgcacaacg agaatgcatt gtgacgccac gtttgacggg
     8401 ggcgaaaaat catctctatg cctgtcattt cccgctgaac atggagaaag agtgagatga
     8461 tcgaaacgct acttgaagtg cgcaatttaa gtaaaacgtt ccgctaccgg accggctggt
     8521 ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt tacgctacgt gaaggccaga
     8581 cactggcgat tatcggcgag aatggttcgg gtaaatccac gctggcaaaa atgctggcgg
     8641 gaatgataga accgaccagc ggcgagttat tgattgacga tcatccgctg cattttggcg
     8701 attattcctt ccgcagtcag cgtattcgca tgatttttca ggatccttcg acctcgttga
     8761 atccccgtca acgtatctcg caaattctgg attttccact gcgcctgaac accgacctgg
     8821 aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg tatggttggg ctgctaccgg
     8881 atcacgtcag ttactatccg catatgttag cacccgggca aaaacagcgt ctgggtctgg
     8941 cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga tgaagccctc gcctcactgg
     9001 atatgtcgat gcgttcgcag ttgattaatc tgatgctgga gttacaggaa aaacagggca
     9061 tttcgtatat ttatgttacc cagcatatcg gaatgatgaa gcacattagc gaccaggtgc
     9121 tggtgatgca tcagggcgag gttgtcgagc gaggcagcac cgcagatgtg ctggcatcgc
     9181 cgctgcatga actcaccaaa cggctgatcg ccggtcattt tggcgaagca ttgacggcgg
     9241 atgcgtggcg taaggaccgt tagtgttttg cacctcatcc tgacaggccc ccctcaggat
     9301 gaggtgttca ttacttttga ttgtcgattc tggtgagagc aaccaacaat gccagcgtat
     9361 aacacaccgt aattgccaga ctcattttta acagtgtttc tccgccaatt cccgacaatg
     9421 gcgcggctat accgccgaag acaaacatgc tcatccccat caacgcagac gccgttccag
     9481 attcctgcgt ccctactgca ctcatagcct ctgccccgcc aacagtgccg atgccgctac
     9541 aaaatgcgat ggtgaaaaat aatgccacca gtgccggaat gggcaattgt acccatgcgc
     9601 ataagaccgt gagcaatgca cacaaaattg ccgctatcag gccacctcgc aacaatgtca
     9661 tcgcgttaat ccgtcgcgcc aggcgcgaga agatccatga agcaatgata agtccgatgc
     9721 cgttaaggcc aaacaccagg ctaaattgca ttggactaaa gccaaattcc ttctgcaaca
     9781 cgaacgacga agagccgata tatgcaaaca aaccagccag cataaaactt tgaatcaggc
     9841 aaaagcgcat aaaacggcgg ttctgcacca cgcttcgtcc ggttaatagc aatgatgagc
     9901 cccttttatt ttctggcaag gtctcattaa taaataacag gcagcccagc aacagtacgg
     9961 tgctaatttc agccattacc cagaataaag tacgccagtc aaaagtgcta acaatgtacc
    10021 cgcccagcac tggcgacaac accggtgcca ggccattcac cgtcattaac agcgcaaaaa
    10081 actgggtcaa cgttactccc tgatatttgt cacgagcaat agaacgagag agcaccgagc
    10141 cacccgcccc cgcgatccct tgaataaaac gccagaccac cagccagtaa atattgttcg
    10201 ttgtcgcgca taaaatggaa gaaacaataa acaatagcaa cgacaagatt aacggacgtt
    10261 tacgccctat tttgtcgctc agagggccaa ataacagttg tccgacgcca agaccaatca
    10321 gtgatgcggt cagggttaat tgcgttatgg tcgtggttgc cgccagttgc tcagacatct
    10381 ccggcaaagc cggcaaataa agatcggtac acatcgggcc gataccggct aacagaccaa
    10441 gaatcaatgc ccatgaaaga gagactctag ccatattatt ccttacttga gtgatgacat
    10501 gccgccgccc agcgactgcc agagcgtgat tcggtttgta aaatcagtca gttgcagtgc
    10561 cagcagctcc tgctgtgcgc tccacaagct gcgctgcgcc gtcaacacgg tcaggtaatc
    10621 accaacaccc gcctgatagc gacgcaagcc aacatcaacc gtttgttgct cagctttaac
    10681 atactggcgc tgtgcatcca gttgctcttc aagcgtggtc cgccgcgcca gtgcgttagc
    10741 gacatcttta aatgcgctct gaacggtttt ttcataggtg gcaatgagcc cacgcttttg
    10801 tgcttctgcg taacgaagct gcgccagatt acttccgcca gtgaacagcg gtaatgtgac
    10861 cgatggcgca aatgaccaga tctgcattcc gtggctgaac aacgacgaca atgcgtcact
    10921 gccaacaccg gcactggcag tcagcgaaat cgtcgggaaa aagttagccc gcgccgcgcc
    10981 aatatctgca ttggcgctct tcaaattatg ttcagcttcc tggatatccg ggcgacgcag
    11041 cagaacatca gatgatactc ccgcaggaac cagacttatc atttgttccg gcaggctttc
    11101 cagtgttccc ggtagcagat tttccgcaag cgttgtacct gccagtaagt tcagcgcatt
    11161 tttatcctgc atcacttgtg tctgatagct ggcaacgctg gcgcgcgctt gctgataaac
    11221 actcatcgcc tcactgacat ctgtcgctgc cgccgtgcca acctgttgct ggcgctggat
    11281 aattttcaat gagttttctg cactggccat cgtttctttt gccagggcca ggttgctgtt
    11341 atcggccgcc agtgtcagcc aggcggtgct gatttcagca atcagcgtta aacgagtgtt
    11401 ctgggctgta aattcactgg caagccaggt ttcccgcgcc gcccgagata aactctgatt
    11461 acgaccaaac agatctagcg tataactgga caccgtgccg tcagcctcgg cagttgttcc
    11521 cgtaccattc gctagcgaac gactgcgagt cgaacttaag gccgcattca ccgtcggaaa
    11581 taacgacgca ttagtttgcc catagagcgc ccgtgctgag tcaatatcag caatcgcttt
    11641 ttgcacatcg cggttactgt tcagtgcaat ggttactacc tgctgcaatc ggggatcgtg
    11701 gatcacctgc tgccagtcat ggctgattgc cttcccctgg ccctgtgctc ccggcaaggt
    11761 cgccgggatt ggggattcag gcgtactgta gtgaggatcg agcgagacac atcccgcact
    11821 caacaacacc agaaaaataa gagaacgacg caacataact tactcctgac ggcgcggttt
    11881 accggcaaat aaacgtttca ccagtacaaa aaacagagga acgaagaaaa tggccagtaa
    11941 tgtagcggtc agcgtgccgc caataatgcc cgtaccaatg gcgatgcggc tgttcgcccc
    12001 tgcgccggtt gctatcgcca gcggcattac ccccgcaata aacgccagcg aggtcatgat
    12061 gattgggcgt aaacgagtct gcgccgcgcg taatgccgca cgactcaagg aatagccctc
    12121 tgcaaccgcc gcttcagcaa attccacaat cagaatggcg tttttcgacg acaagccgat
    12181 agtagttaat agcgctacct ggaagtaaac gtcgttgttt aaatcacgca tccaggccgc
    12241 cagcgccgcg ccaagaagcc ccagagggat caccagaatg accgagaacg ggactgacca
    12301 gctttcgtac agtgctgcaa ggcagaggaa aaccaccaga atagaaactg catacagact
    12361 taatgcctga ccgctggcca gtttctcctg caatgacaaa ccactccagg cccaggttgt
    12421 tcccgcagga agactgtttg ccagttcctc catttttgtc attgcatcgc cggaactaaa
    12481 gccagtcgcg ttttcgccct gaatttcata ggctgccgag ccgttgtagc gcaccagtct
    12541 ttccggtcca tacagccagc gggtggtcgc aaaagcagag aatggtgtca tggcgttatc
    12601 gctaccgcgc acaaaccatt tacctaagtc tgacggcgcg gaacgaaatt cgctgtcgcc
    12661 ctgaatgtag acttttttca cacgaccacg gtcaataaag tcattcacat aagtaccgcc
    12721 ccacgcactg gacagggtgt cggtgacatc attcaaactc aaccctaatg acaccgcttt
    12781 attgctatca atatctacct gcaattgcgg catttgtggc aaatcattgg cgcgtacaga
    12841 atgtaactcc ggactttgat tcgctttttc tatcaactga ttacgcattt gcagcagtgt
    12901 ttcacgatcg gttccaccgt tagctaacaa ttcaaacgta aaaccattgc tttgccccag
    12961 cccatcaacg gctggcggcg tcatcgcaaa taccgtggca tcacgaattg tgcccagctc
    13021 tttggttgcc cgtagggcga tagcctgggc ggtgttttct gcccctttac gttgagacca
    13081 gtttttcaac gaaacaaacg ccatcccggt gttctgtccg ctgccgctaa aactgaaacc
    13141 atcaacggta aagatgacat cggtatttgc tttctcgtta atcaggaacc agtcaacaat
    13201 ctggcgattc acttctgctg tacgggcagc ggttgcaccg gccggcagcg tgtactgcac
    13261 catgatttcg ccctggtctt cagtgggtaa gaaactgccc ggcagtttcc acatcatcag
    13321 ggccattcca ccacccagca cgacataaag ccccatcgtt cgggctgtac ggcgcaggac
    13381 ataaattacg cctcgctgat atttatcttc agtacggcgg taaaagcggt taaatgcgcc
    13441 gaaaaagcct tttttatgtg gtggaacatg ctggaggacg gaaccgcaca gggcgggagt
    13501 caaggtcaat gccaccacca cggaaagcag cattgcggag atgatggtga tggagaactg
    13561 acgataaatt acccccgtag atcccccaaa gaaggccatc ggcaggaata cggctgacaa
    13621 caccagcgca atggcaacca gtgcgccaga aatctcgccc attgattttt ccgtggcttc
    13681 acgcgcgggc aacccttcgt cacgcataat gcgttcgacg ttctctacca ccacaatggc
    13741 atcgtccacc agcaagccga tcgccagcac cattgcaaac aacgtcaggg tattaatcga
    13801 atagccaaac aacgcaagaa cgccgaatgt gcccagcaaa acgacgggga cagctaacgc
    13861 cggaatcagt gtggcacgca ggttttgcag gaataaatac atcacgcaaa ccaccaatac
    13921 gatggcttca aacagcgtct gaattacgtc ctctacggag attttaataa attcagtgct
    13981 gtctttcggg tatgcgatgt cgtatccctg cggcatgttc cgctggaatt cggcaatctt
    14041 atccttgacc agcgtcgccg tattcagcgc attcgcaccg ggcgaaagca ttacagcaac
    14101 cccggcagac gggtgaccgt taagtttgcc gatagcggta taatcttcac tgcccatctc
    14161 aacgcgagcc acatctttta tacgaacaac tgcgccgtct gactggcttt tcacgataat
    14221 atttttgaac tgatccaccg tctgcaaacg agactgcgca cgtaccgttg cggtcagttg
    14281 ctgagtatcc ggtgaaggca atgcccctat tttcccggca gtaacctgca cgttttgcgc
    14341 ttcaatagca ctttgcacgt ctgaaggcat cagcgagtaa gacgccagtt tggccgggtc
    14401 gagccagatg cgcatagcgt attccgcgcc aaagacttgc agactcccca caccttcaac
    14461 acgcgccagg gggtcctgaa cgttactgac cagccagtcg gcgatatccg aactggaggc
    14521 tttgtcagtg gtgtcataca cggcagcaat cagcaaaaag ttgctttgtg atttctccac
    14581 cgtaacaccc gtttgctgca cttcgctggg taggcgcgac tccgcctgct gaattttatt
    14641 ctgcacctga acctgtgcag tatctggatc ggtaccttgt tcaaaggtca cattaatact
    14701 gaccgaacca tcagagctac tggttgagct gaaatagagt aaattatcaa gcccagtgag
    14761 ttgttgctcg ataacctgag tcacgctgtt ctccagcgtt tcggcagaag caccagtata
    14821 agtggctgaa attttaatgg tcggcggtgc aacgtcagga tattgcgcga cgggcaatgt
    14881 gcgaatagcc agaatccccg ccaacataat caaaatggcg ataacccagg caaagaccgg
    14941 gcggcgaaca aaaaagcgcg aaaacatcag gcgttgcctc cattagcctg aacttcaaca
    15001 gccttgacgg tctgccctga agtgactttg gcagaacctt caacaatcag tcggtcgccg
    15061 ttgtgcaggc cgttcagcac cagccattta tcaccatacg tttctcccgt ttcgagcgtt
    15121 cgctgctcta ctttattgtc tttattcacc accagcgcag ttgcattgcc tttagcatcg
    15181 cgcgtaacgc cctgttgagg cgcgagaata gcgtcttcca tcacgccttc atcgacacga
    15241 gcgcgaacaa acattcccgg taataactgc tgttgtggat tggggaaaat tgcccgtaat
    15301 gtcaccgaac cggtagactc atccaccgcg acttcggtga gttccagacg ccctttttcg
    15361 ctgtaggttg tgccatcttc cagaataagt gagacgctca tggtgtcact gttggtcgcc
    15421 agtgactgtt tacgcagacg taataaatcg acactggagc gagtgaggtc gacatacatt
    15481 gtatccagac cacgaatagt cgttaacgct gtatcctgcg acgcggtgtg cgccaggggt
    15541 taccgacgaa atgccaatac gccccgaaat cggtgcggtt accgtggtcc agtcaagatt
    15601 aatgcgcgca gtttccagtg cggctttttt cgcctctaca ctggctttat cttgtgcaca
    15661 ggtagactga gcatcatcag catcctgttg tgaaacaccg ttctctttca ccagtcgggt
    15721 ataacgctgc gctttctggc aatcggcttt taccagtgcc tgtgcttgtt gtaatgctgc
    15781 ccgggcttca ttccatgcag cctgataact ggccgcatca atctggtaga gaggctgtcc
    15841 agccttgacc agatcacctt ccttaaataa gcgtttctgg ataattcccc caacctgcgg
    15901 acgtacttca gcactgagcg cagcactggt acgtccggtt aattcactga ctaccgaaac
    15961 cggttgagat ttgagcgtaa caacgccgac ttctgtttct gacggggatg aattgttact
    16021 ttgcgtatta tcacaacccg ataagagcag cattgccact acagatgttg ctatatactt
    16081 cattattttc tccagggtga gaaataccct cctcattgaa attaatgagg atatgtcagg
    16141 gatattcagt tataacgaac ccgcatcgcg aaatgaattt cgcaatgaaa taagggggat
    16201 taatatcccc cgctattggt taattctgtt cttatttatt tatcaacata ctaacaatat
    16261 cctgatatat ttcctgcaga tgttgtggat cgcttttttg cggtgttaaa cgacgataaa
    16321 ccgtaccttc catcatggta gcaaacactt caacgcagca acgaatgtgc tcgtcgctaa
    16381 gatggggatg cattcttttc atatgtgcac aaccgttagc gaacattctg gcgtcagctt
    16441 cttcaagcat tgccataacc cgcgggttgc gtgtggcttc tgcagccact tccagcatca
    16501 gcgcgtcgtc gtcctcattt aaggatttac gcaaggcaag gacttccggg aagtgatcgg
    16561 tacgcgcgtc aatgtccatt tgggcaatac gaaagtcgat gatacgacga accatctctt
    16621 caataatggc gtctttgttg gcgaagtaac gatatatctg acccacgcta agctgagctt
    16681 cgctggcaat ttgcgacata ctggcggcat gaaatccgca gcgacgaaaa caccgacggg
    16741 cggcgttaat gatttcatcc tgacgctgtt tgtggcgtat ctccagcttg ctggtcattt
    16801 ctgtctcctg gcctatcact tgatgagaat gatcgttctc atttttgcta attatacgtt
    16861 tctccaaagt aatggaaagt atttttatct tattcatgat gcatattctg ataatgaaga
    16921 gattgtggag attctgtgaa gatagttgac aatattaagt tggggtcaat tcatcagaaa
    16981 gacagcgact tgttgtcgcc agttctgttt tcatcatcaa atttatcgga gctaatacga
    17041 atagtttcat agtcagattc tataagatat ttccccaacc tcccccttgc aggcgtcagg
    17101 agtagataac gcaaaatgct ctgtaccgca gcttctctcc gctacagaaa gcgcaactat
    17161 agccacccac agccaggttg attataataa ccgtttatct gttcgtactg tttactaaaa
    17221 cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta aagctatggg
    17281 ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactgt ccatcgccta
    17341 cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct accagaacga
    17401 caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca tcgttctgca
    17461 gtgcgatgtt gccgaagata ccagcatcga caccatgttc gctgaactgg ggaaagtttg
    17521 gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc agctggatgg
    17581 tgactatgtt aacgccgtta ctcgtgaagg cttcaaaatt gcccacgaca tcagctccta
    17641 cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt ctgccctact
    17701 gaccctttcc taccttggcg ctgagcgcgc tatcccgaat tacaacgtta tgggtctggc
    17761 aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc cggaaggtgt
    17821 gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg gtatcaaaga
    17881 cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta ccgttactat
    17941 tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg gtatctccgg
    18001 tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac tcgaactgaa
    18061 ataatcgttc tgttggtaaa tattgggcgg cgttctgccg cccgttatct ctgttatacc
    18121 tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc gtcaggataa
    18181 cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg gcaaaagcca
    18241 ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc tggtcccgga
    18301 agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag aaacgctggc
    18361 acccttcctt tcatggctgg tccctgcgcg cgttcttgca gtagagttgt tccctgacca
    18421 gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcga cggccctgac
    18481 ggttgctcag gtttgcggcg tccaacggtt atgtaactac tattcggcgc gacttacgcc
    18541 gctccccggg cctgattcct ccagggaaag taatcatcgg ttggcacaaa tcacgcaata
    18601 tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc agcatctgaa
    18661 tgacgttggt cttactgtct gtgactgtgt aatcattaac caaatcattg gttttattgg
    18721 ctttcaggcg cggacaattg cgacatttca ggcttatctc gggcatccag tacgctggtt
    18781 acccggtctg gagatacaaa actatgccga cgcgtcactg tttgctgatg aatcaatacg
    18841 ctggcgaagc agctatgagg tggaaaaact ccctgaagag cacacaaaaa gttcaactgc
    18901 agaactttgc caactggccg aaacactctc tctccaccct atttcacttt cccttctcga
    18961 aaagttgcta aacagcacac gggtcaatac acagccggat aatcagcttg cggcgttgtt
    19021 atgcgcgcgg ataaatggca gtcctgcttg ttttgccacc tgtatggatt catcaaatga
    19081 atataaaaaa atcagcgccc ttctgcgcaa aggcgaaaat gaaattaacc aatgggctga
    19141 ccgtcattct gttgagcgcg ctaccgttca agcgatacaa tggctgaccc gagcacccga
    19201 tcgctttagc gccgcccagt tcagcccatt actcgaacac gaaaaatcat caacgcagat
    19261 tattaatctg ctggtatgga gcgggctgtg tggctggata aatcgcttaa aaatcgcgtt
    19321 gggtgagaca tattaacctt gccgcgtcag acagattcgc gtaaaactgt cagccgctct
    19381 aatggccacc aaaatagaca attatgtttc aggacaaccc gctgctagcg cagcttaaac
    19441 agcaactgca ttcccagacg ccacgcgctg aaggggtggt aaaagccaca gaaaaaggct
    19501 ttggcttcct ggaagtcgac gcgcaaaaaa gttatttcat tccgccgccg cagatgaaaa
    19561 aagtcatgca tggcgaccga attatcgcgg tgatccacag tgaaaaagaa cgtgaatccg
    19621 cagagccaga agaactggtt gaaccgttcc tgactcgttt cgtgggtaaa gttcagggca
    19681 aaaatgaccg tctggccatc gttcctgatc atccactctt aaaagacgcc attccttgcc
    19741 gcgcagcccg tggcctgaac cacgagttta aagaaggcga ctgggcggtt gccgaaatgc
    19801 gccgtcatcc gctgaaaggc gatcgttctt tctatgctga actgacacaa tacatcactt
    19861 ttggtgacga tcactttgta ccgtggtggg ttacccttgc gcgtcataac ctggaaaaag
    19921 aagcaccaga cggcgtcgct accgaaatgc tcgatgaagg tctggttcgt gaagatctga
    19981 ctgcgctgga ttttgtcacc atcgacagtg ccagcacaga agatatggat gacgcccttt
    20041 tcgccaaggc gttaccggat ggcaaacttc agctgattgt ggcgattgcc gatccaaccg
    20101 cgtggattgc tgaaggcagc aagctggaca aagccgcgaa aattcgcgca ttcaccaact
    20161 atctgcctgg cttcaacatc cctatgctgc ctcgcgagct ttctgacgat ctctgctcac
    20221 tgcgcgccaa tgaagtccgc ccggtactgg catgccgcat gacgctctcc actgatggca
    20281 ccattgaaga taatatcgaa ttctttgccg ccaccatcga atccaaagcg aagctggtgt
    20341 atgaccaggt ttctgactgg ctggaaaata ccggtgactg gcagcctgaa agcgaagcaa
    20401 ttgccgaaca agtccgtttg ctagcgcaaa tatgccaacg ccgcggcgag tggcgtcata
    20461 accacgcact ggtgtttaaa gatcgcccgg attaccgctt tattctcggt gaaaaaggtg
    20521 aagtgctgga tatcgtcgcc gagcctcgtc gcattgccaa ccgtatcgtc gaagaagcga
    20581 tgattgccgc taacatttgt gcggcccgcg tactgcgcga taagctcggt tttggcatct
    20641 ataacgtgca tatgggcttt gatccggcga atgccgacgc gttggcagcg ttgctgaaaa
    20701 cgcacggtct gcatgtcgat gctgaagaag tgctcacgct ggacggtttc tgcaaactgc
    20761 gtcgtgaact ggatgcgcaa ccaactggtt tcctcgacag ccgcattcgt cgcttccagt
    20821 catttgctga aattagcact gaacccggtc ctcactttgg cctcggtctg gaagcatacg
    20881 ccacctggac ttcgccgatc cgtaaatatg gcgacatgat caaccaccgt ctgctgaaag
    20941 cggttatcaa aggcgaaact gcgacgcgtc cacaggatga aatcactgtc caaatggccg
    21001 agcgtcgccg tctcaaccgg atggcagaac gtgatgttgg cgactggtta tacgcacgct
    21061 tcctgaaaga caaagccggg accgacaccc gtttcgcggc ggaaattgtc gatatcagcc
    21121 gtggcggcat gcgtgttcgt ttggttgata acggcgctat cgcctttatt ccagcacctt
    21181 tcttacacgc tgtgcgcgat gaactggttt gcagccagga aaacggcact gtacaaatta
    21241 aaggtgaaac ggcttacaaa gtgactgacg ttattgacgt caccattgcc gaagtccgca
    21301 tggaaacccg cagcattatt gcgcgcccag tcgcgtaatc tcctttcacg gcccattcct
    21361 catggatggg ccgtttattt ccccgctcaa ccttcctcat atcccggcag atttttaact
    21421 gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat atataaaaaa
    21481 aacaatgtgt ttagccgccg ttaatttcat gtaagtcatg ttgttccttt tatttaactg
    21541 cggactccgc tgttaaccgg aggatatgca tcatgaaaac cgttagggag tccgcaacgt
    21601 tgtacaactt tctcggatcg cacaatccat actggcggtt gacggaaagc agcgatgttt
    21661 tgcgcttttc taccaccgaa atcacagaac ccgatcgtat tttgcaacta tctgccgaac
    21721 aggctgctcg catcagggaa atgacggtca tcacctccag cctgatgatg agtctgaccg
    21781 tcgatgaaag cgatctttct gtgcatctgg taggacgaaa aatcaataaa cgagaatggg
    21841 gaggcaacgc gtctgcatgg catgacacac cggcggttgc tcgtgattta tcacatgggc
    21901 tttcctttgc tgagcaggta gtttctgaag cacattccgc aatagtgatt ctcgacagcc
    21961 gggggaatat ccagcgcttc aatcggttat gtgaagatta cacagggttg aaagaacacg
    22021 acgtcattgg gcaaagcgtg tttaaactgt ttatgagtcg tcgtgaagct gcggcatcca
    22081 ggcgcaataa ccgtgtattt tttcgaagcg gcaatgcata tgaagtcgaa ctatggatac
    22141 caacacgtaa aggccagcgg ctgtttctgt ttcgcaataa atttgtccac agcggcagtg
    22201 gcaaaaacga gattttttta atctgttccg gcaccgacat taccgaagag cgccgcgctc
    22261 aggagcgact gcgtattctg gcaaataccg acagtatcac cggactgccg aatcgtaacg
    22321 cgatgcagga gttaatcgat cacgctatta atcaggcaga taacaataaa gttggggttg
    22381 tgtatcttga tttggataat tttaaaaagg tcaacgacgc ctatgggcat ttgtttggtg
    22441 accagttatt acgcgacgtg tcattggcta ttttaagctg tctcgaacat gaccaggtat
    22501 tggcgcgtcc aggtggcgat gagtttctgg tactggcatc caatacctca caaagcgcgc
    22561 tggaagcatt ggcatcacga attttgaccc gcttacgact cccctttcgc attggtttaa
    22621 ttgaagttta taccagctgt tcagtaggta tttcactctc tcccgaacat ggttcggaca
    22681 gcgcggctat tattcgtcac gccgacacag caatgtacac agcgaaggaa ggcggacgag
    22741 gacaattttg cgtttttacc ccagaaatga atcagcgggt atttgaatat ctctggctgg
    22801 ataccaactt gcgtaaagca ctggaaaacg atcagttggt tattcactat caaccgaaaa
    22861 tcacctggcg tggcgaagtg cgcagtctgg aagcactagt acgttggcag tcacctgaac
    22921 gtgggttgat tccaccgttg gacttcattt cctacgccga agaatcaggg ctaattgtgc
    22981 ctttaggccg ttgggtgatt ctcgatgtcg tacgccaggt agcgaagtgg cgggataaag
    23041 gcattaacct gcgagtggcg gtaaatattt ctgcacgtca gctcgccgat caaaccattt
    23101 tcaccgccct gaaacaggtt ctccaggaac tcaattttga atactgccct atagatgttg
    23161 aactgacgga gagctgcctg attgagaatg atgaactggc actgtctgtt attcaacaat
    23221 ttagccgatt aggtgcgcaa gttcatctgg acgattttgg taccggctac tcttcacttt
    23281 cgcaactggc gcgctttccg atcgatgcca tcaaacttga ccaggttttt gttcgggata
    23341 ttcacaaaca gcctgtctcg caatcactgg tccgggcgat cgtcgctgtg gcccaggcat
    23401 tgaatcttca ggtgatcgcc gaaggtgtag aaagtgcaaa ggaagatgct tttttaacca
    23461 agaacgggat caatgagcga caaggatttt tgtttgccaa accgatgccc gccgttgcct
    23521 tcgaacgctg gtataaacgc tatctgaagc gcgcataagt agatgaatat atatacttag
    23581 cactatattc cgtcattata tatcgtattc ccggatgata attagccgtt gctgttaaac
    23641 acttctcttt tagcggcaat ttttgcatta ttcattttcc cccttttgct ataaggatgt
    23701 ccttgatgtc tgaatttgat gcccagcgcg ttgccgaacg tattgatatt gtgttagata
    23761 tccttgtggc aggcgattac cactctgcga tccataatct cgagatactt aaagcagaac
    23821 tcttgcgcca ggttgctgaa tctacgccag acatcccgaa ggcaccatgg gagatctaac
    23881 aatcgtgcct tcaggttttc ctatttgagg atgcccggcg cgtcagtatt tttttattta
    23941 gtattataac gttataagaa ttacagcgat gaattcccga caacaaacta ttctacagat
    24001 ggtcattgac cagggtcagg ttagcgtaac cgatctggca aaagccactg gagtttctga
    24061 agttaccatt cgccaggatc tcaacaccct cgaaaaactg agttacctcc gacgtgcaca
    24121 tggttttgca gtttcgcttg atagtgatga cgtcgaaacc cgtatgatga gcaactatac
    24181 gctgaagcgt gaactcgccg agtttgccgc gtcactggtt caaccgggcg aaaccatctt
    24241 tatcgaaaat ggtagcagca atgccctgct cgccaggact ctgggcgagc agaagaaaaa
    24301 tgtcactatc atcacggtca gcagctacat cgcgcatttg ctgaaagacg cgccttgtga
    24361 agtcattttg ctcggtggcg tgtaccagaa aaaaagcgaa agtatggttg gccctttgac
    24421 acgccagtgc atccaacaag tgcatttcag caaagcattt attggtattg atggctggca
    24481 acctgaaact gggtttaccg gtcgcgatat gatgcgtacc gatgtggtca atgccgtgct
    24541 ggaaaaagag tgcgaggcga tagtcctgac tgacagctcg aaatttggtg ctgtacattc
    24601 atactccatc ggccccgttg agcgattcaa tcgcgtgatt accgattcga aaatacgcgc
    24661 cagcgatctg atgcatcttg aacaaagtaa actcaccgtt cacgtcgttg acatttaacc
    24721 atttcccttc tctacggatg atttgcagtt tggcaaatca tccgctctaa gatgattcct
    24781 ggttgataat taagactatt tacctgttat taacactctc aagatataaa attattatca
    24841 gcgatataac aggaagtcat tatcacctgc gtgatataac cctgcgcgcg agcggatttc
    24901 acggaataat ttcaccaggc ttattcttag ctattatagt tatagtgagc ttacttccgt
    24961 gaatcataaa ttcaggagag agtattatgt ttgtaacgag caaaaaaatg accgcggctg
    25021 ttctggcaat taccctggca atgtctctga gtgcctgttc taactggtct aaacgagacc
    25081 gtaacaccgc aattggcgcg ggtgcaggtg cattaggcgg tgcagtactg accgatggca
    25141 gtacgctggg tacattaggt ggtgctgccg tcggtggtgt tattggtcac caggtcggta
    25201 aataatcgta aataaatgtc gattatcgac tcgcaattat aatacgcttg attctgttca
    25261 gtacgaaata actgaataat acacacagta aaatattcga aagccacgga tatatccgtg
    25321 gctttttatg ttaaccgcct gcgagtttta ccttcatccc tttcgcttca agcagtgatt
    25381 ttaataaatc acgcttatcg ccctggattt caataattcc atctttaact gctccgccgc
    25441 agccgcattt tttcttcagt tccgctgcca gtttcgtcag ttcggcatca tcgagagcga
    25501 caccggtaat caggcaaacg cccttacctt tacgtccact ggtctgacgc tgaatacgca
    25561 ccacaccgtc gcctttaggg cgtacagggg ccgcttttgg ctcatcaata cgtccggttt
    25621 ccgttgagta gaccagacgg ctgttggaat cactcatcat gcactccgct gtaaagaggc
    25681 gttgatcgct ttcagcgtct gcgctggatc taccgattgc gttaccgggc gaccaatcac
    25741 catataatca acaccagccg ccaacgcctg ttctggcgtc ataatgcggc gctggtcacc
    25801 agcatcactc ccctgcggac gaatgcccgg cgtaaccagt ttgaactcct gaccgaatac
    25861 ctgtttaaag cgcacagctt cctgagcaga acacaccaca ccatcaaggc cacatttttg
    25921 cgtcagtgcc gccagacgtt ctgcataatc tgcaggtgac agtgtcacgc caagatcggc
    25981 caggtcgctg gcttccatgc tggtcaacac tgtcacagca atcaaaagcg gtgcatcttt
    26041 gccaaacgga accagtgcct cacgcgctgc ggtcatcata cgcgccccac cggaggcatg
    26101 aacattcacc atccacacgc ctaagtcagc cgcagcagcg acagcgtgcg ctgcagtgtt
    26161 gggaatatcg tggaatttca ggtcaagaaa gatatcaaaa ccacgctgtt gaagttcgcg
    26221 cacaaactgt ggcccaaaca atgtaaacat ctctttgccg accttcagac gacaatcgcg
    26281 tgggtcgatc ttgtcgacaa aggccatcgc ggcatcgcga ttatgataat caagggcaac
    26341 aactacagga gaattcgtaa cagcgcggga agaagatgaa gcagttaacg tcatgaccag
    26401 accttcttga tgatgggcac cggaagtggc gcgaaacagg taaacggcga gcattctacc
    26461 cgtggaaata aaaaattaac agttgcgatt tctcctctgg caggcttttc cgcgccgtcg
    26521 gtgccggagc acaatagaac atcaataatt agtatgttgt aactaaagtc ggattttttt
    26581 aaaaattaca ggccatcaag accgcgaatt ggtttaatgg ttgaccaggc ccgacaagac
    26641 ggacaatgcc agtagagggt gtatgcggta aaaccacatt tctggcagcg ataacggggc
    26701 ttactacgta ccttctcgcc aaccatgtca cgcagcacca tcagactctc tttggcacgc
    26761 ccttcttctg cttcatttaa gtggtaatcc attaacttat ggaacacacg catggtcgga
    26821 tgacgctgaa gctggcgagt aatatagacc tgtgcggcct cactaccgtc gcgcgcttcg
    26881 atgatatccg caagcatcaa ttcagcatcg gcaccggtgt tctcttccac cgcgcgctgc
    26941 aggaattctg cccattcggc agttttaccc aactgctgat agcatgtttg cagcatttcc
    27001 agcgtttcgc tgaccagttc tctgtcctgg gatatgacac gttgcaaact ttcgacggct
    27061 ttggcgtatt ctcctttcgc cataaacacg cgtcccatca tgatggatac gcgggcgctg
    27121 tttttatctg ccgccgcacc tttttttagc aaagtcatgg cacgatcgag atcgtcgctg
    27181 gccatatgct gcagagctaa ctcacagtag aaatgggcaa tttcgacgcg ctgtttatct
    27241 ttacccagct tcaccaggcg ttcggcaaca tcaattgctt tctgccactc gctggtagcc
    27301 tggtaaattt gtagcagttg ttgcagcgcg ccaatgcgga agtcagtttc atctgtcagc
    27361 tgattgaaca tgtcttccgc gcggtcgtat aacccggcag ccatgtaatc acgccccaat
    27421 tgttgaatcg ccaaaagccg ctgttcatag gtcagcgagg cgctttccat tagggtctga
    27481 tggatgcgaa tagcgcgatc aacttcgcca cgcgaacgga acaggtttcc gagcgtaagg
    27541 tgggcttcaa cggtacctgt atcctcttta agcatatcga gaaacaggtc taccgcttta
    27601 tcctgttgat tactaagcag gaagttaacc cccgctacgt aatcacgcga caagcggttg
    27661 gcttcatctt gcttgttttg ttgcgcactt ctgcggccca tataccagcc ataggcagcg
    27721 gctacaggca aaagcagaaa caacaactcc agcatagaaa gttattcctt cgacgcagac
    27781 gagtgcggcg ctacagccac gtcagtcgcg ggtgaaagct ggttttccag tcgctttatt
    27841 ttacgttcag cgcgcgccag ggaaacacga actcgcagcc agaacaggcc acaaatcaac
    27901 caaccgatag caaaccccgc agcaaacaat accgccagca atgtggaaat acggtactcc
    27961 ccttgcgcta acagataatt aaacgtcacc tgttgatcgt tctgcgcacc caacgtgacc
    28021 gaaatcacga agatcgctaa caccagtaag aaaatgagta aatatttcac attacttccc
    28081 gttatgtggt tagagcgaat aaattatggt caacttaccg caatagccct ttaaagtacc
    28141 attttcgcgc cgtctgcgaa atggcatcca cgcgcattgt tgatgattta agggctaaat
    28201 tcggaaaatc aaccaacgtt aactttcttg ttctcgttgt gcaatttcgc gattttcttc
    28261 cgcaggtggt gttaatggcc cacaaattcg ttgcgcaagc cacgttgcca ccgccaccag
    28321 cgcccacgaa atcaacgtag ctacaaccag atcgcgtggc caatgcatcc cgagcagcag
    28381 gcggcttccc atgactcccg ttgcccagac caacaagata gcaatggtta acgttcgccg
    28441 acgcggccac agcaaaccaa cggccagcag tgcccaactg gcagcaaaca tcgtgtgacc
    28501 ggaaggaaag gcaaaccccg tctctttctg ccagtgtgaa cgcaaatatt gtgggatatt
    28561 tttctcttca gccaactgtt ctttcaccag atttccgcgt tctgctcgct ttaaagtgta
    28621 gaactcatca accggaatat gatgtgtttt ttccagccag ataacaaaag gtcgtggttc
    28681 ctggactttg tctttgatcc aggatttaac gccttgtccc ataaggattg ccgccgccag
    28741 aatggcaaat aacataatgg cagccttaat gcgaaaacgc agacaccaga gaaaccagcc
    28801 gaataaaatc aaatgtgtaa tgacgcccca gggctgggtg acagtttcag taacccaaaa
    28861 agccgctttt agtaaccaac tttgttctcc aggttgccaa cgccagccag aaagccatac
    28921 ggctactggc atgacaagca atagtgcagc tcccactgcg gtacgtctgg caatcgaacg
    28981 catggcctct cctttttgat aagtcccaca atcataactg aaaatgccag ttccaggaaa
    29041 aattgacaga tttgtgccat tccgtgaacg atcgacgcgt cgtgattagg tgaacccctt
    29101 ctcgttatgg caaaataagc caatacagaa ccagcattat ctggagaatt tcatgcagct
    29161 taaacgtgtg gcagaagcca aactgccaac cccatggggc gatttcctga tggtgggatt
    29221 tgaagaactg gcaaccggac acgatcatgt cgcgctagtc tatggcgata tttccgggca
    29281 taccccggta cttgcgcgcg tccattccga atgtctgacc ggtgacgccc tgttcagctt
    29341 gcgctgcgat tgtggcttcc agctcgaagc ggcattgacg caaattgccg aggaaggccg
    29401 tggtattttg ctgtatcacc gtcaggaagg tcgtaacatt ggtctgctga ataaaatccg
    29461 cgcttacgca ctgcaggatc aaggttacga tactgtagag gctaaccacc agttaggctt
    29521 cgccgctgat gagcgcgact tcactctttg cgctgatatg ttcaaactcc ttggcgtcaa
    29581 tgaagtccgc ttgttaacca ataacccgaa aaaagtcgaa attctgaccg aagcagggat
    29641 taatattatt gaacgcgtac cgttgattgt aggtcgtaac cccaataacg aacattatct
    29701 cgataccaaa gccgagaaaa tgggtcattt gctgaacaaa taaccctctt gcattgtgta
    29761 attgatttgc ttgccggaag caaaataacc ggcaagcaaa tactcgttac ttcaacatat
    29821 tacgaatgac ataatgcaaa atgccgtcgt tctggtagta ggtcaactcc gtcgtggtgt
    29881 cgatacgaca acggcagggt acgacttcct ggctaccatc cgcgcgcgta agcgtcaccg
    29941 gaaccgtcgc gccgggttgt agattttgca gatcaccaat atcaatcttc tcttccccgg
    30001 taagctctaa cgttttacgc gtcacacctt gtggaaattc cagcggcagg atgcccatgc
    30061 caattaaatt cgaacggtga attcgttcaa acgattcggc aatcacaaca cgaataccaa
    30121 gcagacgcgg accttttgcc gcccagtcac ggctggagcc tgatccatac tctttcccgg
    30181 caatcaccgc cagcggcgtt tgctcctgct tatagcgcat tgcggcatca taaatagaga
    30241 cgacatcgct gtcaggtaaa tggcgcgtca tccccccttc aacgccaggc accatttcat
    30301 tacggatgcg aatattggcg aaggtgccgc gcatcatcac ttcatggtta ccacgccgcg
    30361 aaccatagga gttaaagtct ttccgctcaa caccccgacc ttgtagatat cgacccgccg
    30421 ggctgtcggg cttaatactg cccgccggag agatatggtc agtggtgact gaatccccca
    30481 gcattgcgag gatccgcgca ccgtgaatat cttccacagg tgctggtgtt gcctgcattt
    30541 catcaaagaa aggcgataag cgaatatagg ttgaatcctc ctgccaaccg taggtatcgg
    30601 atcgtgtgac gttaatttcc ttccactcgg ctgtgccttc aaaaacttct gcgtactctt
    30661 tgcggaacat ttctgtggag acttggtcta ccgcacgggc aatttcttgt gccgatggcc
    30721 agatatcttt cagatatacc ggctcgcctt tgcgatcatg gccgataggc tcagaagcca
    30781 ggttgatatt catatttccc gccagcgcat aggcaaccac cagcggcggc gaggccagcc
    30841 agttggtttt aaccagcgga tggatacggc cttcaaagtt acggttgccg gacagcactg
    30901 caccgacggt taaatcgcct tttttgattg ccgtttcgat aggatcgggc aacggtccag
    30961 agttaccaat acaggtggta caaccgtatc ccacaaggtt aaatcccagt tcatcgagat
    31021 acggtgtcag tttcgctttt gccagataat cagaaacgac tttcgaaccc ggtgccagcg
    31081 acgctttgac ccacggttgc cgcttgaggc ccagagttac ggcttttttc gccagcaagc
    31141 ctgcggccat cagcacactt gggttagagg tgttggtgca cgacgttatc gcagcaatga
    31201 ccacagcgcc atcaggtaac tgatactgat gtccgttcat aacataatcg accggcaggc
    31261 gatctttatg cgtggcattc acttccagtt cgctactggc agcaaatgct tttggtacat
    31321 cgggcagtgc aacgcgatcc tgcgggcgtt ttggccctgc aaggctcgct tcaacgtcat
    31381 tcatatccag ttctaacaca ctggtaaaaa ttggttcatc gcccggatta cgccacatgc
    31441 cctgcgcttt ggcatatttt tcgaccaact cgatctgatc ttcactgcgc ccgcttaaac
    31501 gcatgtaatc gagggtgaca gcatcgattg ggaagaagcc acaggtggca ccatattctg
    31561 gcgacatatt ggcaatggtg gcgcgatccg ccagcggtag tgaatccagc ccatcaccat
    31621 aaaattcgac gaatttcccc accacgccat gtttgcgcaa catttgggta acagtgagaa
    31681 ccaggtctgt ggcggtaata ccttcacgta attttccggt aagtttaaag cccactacgt
    31741 ccgggataag catggaaacc ggctggccta acattgcggc ttctgcttcg atgccaccaa
    31801 cgccccaccc cagcacgcca aggccgttga tcatggtggt gtgcgagtca gtaccaacga
    31861 gtgtatccgg ataagcaatc cattcaccgt cctgcaattc gctccacact gctttgccga
    31921 gatattcgag gttaacctga tggcaaatgc ctgtgcctgg cggcacgacg ctaaaccgac
    31981 tgaacgcctg ctttccccat ttcaggaaca cataacgttc gtggttgcgc tccatttcca
    32041 ggcgtacgtt ttcttcaaat gcctcatcat caccaaaacg atcgacggtc accgagtggt
    32101 caatgaccag gtcgaccggt gagagtgggt taacctttgc agtatcgccg ccgaggcgtt
    32161 taaccgcttc gcgcattgcc gccagatcaa caacggcagg tacgccggta aagtcctgca
    32221 tcagcactct tgccgggcgg taggcaattt cacggtcagc atgggcattt ttcagccatc
    32281 ccgccagcgc gtggatatcc tcttcggtaa ccgatttacc atcctgccaa cgcagcaggt
    32341 tttcgagcaa aactttgagt gacttgggta gacgggtgat atcgcccagt gatttggcag
    32401 caagctgtag tagtggtaag ttttatcttt ggcctgcaac gtgtccttac tggcttctcg
    32461 tagggttgac gacatagctc ctccttaatg acagggttgc gaagccctga ttctcttcag
    32521 ggtgaatatt aaagataaca caaacatcgc gtaacgattt gataacaacc caaattgata
    32581 aaagaggaga aaccttgagg caaagcaaac agcggggtca gggatcagtg agtaatgatc
    32641 cagaggagtt cggcacagaa gagcatcgac agagaaaatg cgcccatcca ggaccaatat
    32701 ttcatatttg tgttgatcat aggctacacc cttaacggtt attactttgt gttgagataa
    32761 ctcaacctgt tattgctgcg gtgtattaaa agccctgtaa ttcattaaaa atgggtaata
    32821 agaatgatga taattattgt ttcggctgat catcttcagc aaacagttct ataaaggctt
    32881 gttgctgcga tgttagttgc cagtcgtctg gaataacgac ggaacattcc cgttttgctt
    32941 ctggttcatt gtcactatgc tgacataatg gtgacgactc cagttgctcc tgaccgacca
    33001 tagttatccc cacaatttcc aaataaacag tgcgacaacc acccagaaaa gtgcagaaac
    33061 aagaaaaacc gccagccagg ctttacgttt taattcagga ttgcggcggg gttcctgatt
    33121 tccagaaggc attgctaacc tcatataatc gccatcactt atcagcaaga cgttgaacga
    33181 ttggtgtaat taatcatacg atgagacaaa tcctaaagaa actttagtca aaaatccagc
    33241 gaatttacga atcgtctcca gagaataatt caa
//
LOCUS       DAZGJT010000045        32992 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZGJT010000045 DAZGJT010000000
VERSION     DAZGJT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32992
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.045"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1505
                     /locus_tag="ACHKFK_002356"
     CDS             <1..1505
                     /locus_tag="ACHKFK_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HFO9052681.1"
                     /translation="PQSHITYVNPDFIKISGFTEEELLGQPHNIVRHPDMPPAAFEHM
                     WSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPIAKNGSIVEYQSVRTKPEPEQVLAAE
                     KLYAQLRSGKAARPKLAASFSVKILLLIWGSIISSAMAAGMLTDTSISSLLLATLMSG
                     SLSSVSVLAILSPLGRLVERARNISNNPLSQSLYTGRTDEFGQIEFALRMMQAETGAI
                     VGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQAETDQIATAVNQMVASIQEVASNAQ
                     HAADAAGRADTETASGQRLVAHTSQSITALEGEIRQATQVIHELEGQSNEISKVLDVI
                     RGIAEQTNLLALNAAIEAARAGEQGRGFAVVADEVRSLAARTQQSTTDIQSMISALQE
                     RAQSAVTVMEQSSRQAHTSVAHAEEAATALDGIGQRVNEITDMNAQIATAVEQQGAVS
                     EDINRSIINIRDAADTNVQTGQNNLQSAKSVAQLTSALSELAKQFWEKRG"
     gene            1964..3463
                     /locus_tag="ACHKFK_002357"
     CDS             1964..3463
                     /locus_tag="ACHKFK_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HFO9052682.1"
                     /translation="MNVEESEQRWVCACCIGEEFLRHKVEKEGRIQTCDYCDEIHTCF
                     SLEEVCDLTEKAIEAHFYRTDTEPNDMEYASLRHIDGYEWFREGENVVQLIEDLLQSR
                     RALADDIQQLLEYRHSDFDSDVMGLETEFARESCYAERKQISTGRLDSMWINFVTSLK
                     TESRFINNAVSSTLDGIFQGVETMRARGKEPVIIDAGPGTAIPSLYRARWCRNHHELE
                     QILVTPDKELGPPPHQFSGSNRMSAKGISVFYGASSVDTAISEIRPPVGCKVVCAKFS
                     LIRSLRLLNLPALESVLESGSLLDPDYIKRREQAAFLSTFTSRIVEPVLPGEEDFSYI
                     PTQVIAEYLADPLRFNLDGILYPSVQLPGADQEERYNVVLFHKASRVAFMTLPAKDDC
                     MIRYGHQYAEDEWETDICVTEVISAQEDPPPARHDLSDLSLPDDPREPALEIDLDSVS
                     VHGIRAARFEYSTDAVRRSTYHYTPRTSEPAGTITQLWDTASGHDDFPF"
     gene            complement(3488..4351)
                     /locus_tag="ACHKFK_002358"
     CDS             complement(3488..4351)
                     /locus_tag="ACHKFK_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052683.1"
                     /translation="MKDQRIELRLPQQQLDELDNFINNIDGQYKPSRSDVLRSFIAQG
                     VRGKFTPASQEAEMFPLSARLNIFFQLCQLLRMECGKDGRSVQPINPTYGYNNRVAST
                     VTAEALVRQVYLQRMTWFFELDAVHLQAINPNLGQDMIVSLMNPQPSPVICNTLDSVI
                     ALRDMFSNISMVLAEAEKTVNDWNDQKTRDALARIQGYVKDNGLQLTFKGYPDTEDYA
                     LQIDMWSLLNWIDNGQGNHRIGDYGLRNDKDLTDKYAVMLEVYQNIRSNHQFDLNGLE
                     QMVKSRQFHMI"
     gene            4874..5287
                     /locus_tag="ACHKFK_002359"
     CDS             4874..5287
                     /locus_tag="ACHKFK_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052684.1"
                     /translation="MFFIENEGQAVAGTDYWQSVQAQAGYVYLSWNAGAARLLVPDAA
                     KHLLREMRGAEYVIISKGALHGRDALELVFEDGSDAPFVIHMLSEQCDRLLPENNQGG
                     GFVVTVWTRGGNQLRYPGKYRVVENLPDVSPWSEH"
     gene            5289..6068
                     /locus_tag="ACHKFK_002360"
     CDS             5289..6068
                     /locus_tag="ACHKFK_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9052685.1"
                     /translation="MQHLPAPIHHARDAAQLPVAIDYPAALALRQMSMVHDELPKYLL
                     APEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPFVQLATLKQR
                     TEKAARTAGRMPVGQQTHRLVPLSDAWYVSQLQTMVATLKIPMERRNKRTGRTEKARI
                     WEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIPLKVLQSLMG
                     HKSISSTEIYTKVFALDVAARHRVQFSMPESDAVSMLKRIP"
     gene            6248..6892
                     /locus_tag="ACHKFK_002361"
     CDS             6248..6892
                     /locus_tag="ACHKFK_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO9052686.1"
                     /translation="MPVIANTHPKGGVGKTTSSVNIVGEMKSDTVDLDTHTGLSIILG
                     LRPEGKEISVKVPKTVDELIEIMTPYKNSDKTLLIDCGGFDSDLTRTAIAFADCVIVP
                     SKDSLTERIGLMHFDGVLDEISSIMGTDITAHLYLCKVNPNKKKFPKLDAILPSFKHL
                     KLMKSRISARAEFEDVIETGMGITESVHGRYSAGGKEVIALIEEINHLIENNKQ"
     gene            6979..7287
                     /locus_tag="ACHKFK_002362"
     CDS             6979..7287
                     /locus_tag="ACHKFK_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone GroEL"
                     /protein_id="HFO9052687.1"
                     /translation="MSKAKFSAQAEKLKNLVDSARLQTSSSAVTATAPSQPNEGAEEV
                     SGRPVAARTKAKNLKAIPLSYFEAHAELKATSKTSLDFSSYIIEAIREKLERDGAIGQ
                     "
     gene            7701..8681
                     /locus_tag="ACHKFK_002363"
     CDS             7701..8681
                     /locus_tag="ACHKFK_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO9052688.1"
                     /translation="MKIFIDDGSTNIKLAWLEDGDVKTLISPNSFKPEWSFSLLDDAA
                     PANYEIDGEKFSFDPLSADAVVTTETRYQYSDVNVVAIQHALQQTGLKAQPVDVIVTL
                     PISEYLDANNQKNKQNIERKKKNVMRQVRVQGSDAFVIRSVSVLPESIPAGFSVLAGL
                     EDDESLLIVDLGGTTLDVSHVRSKMTGITKTWCDPNIGVSLITSGVKEQMAVHANTRV
                     SSFQADNIIVHRNEPDYLSRRIYNAEQRESIINVINERQKLLIKRVNDVISRFTDYTH
                     VMCVGGGAEIVAEAVKNLTKVPDERFYLSSSPQFDLVMGMIKMKGGVTNE"
     gene            8674..9090
                     /locus_tag="ACHKFK_002364"
     CDS             8674..9090
                     /locus_tag="ACHKFK_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9052689.1"
                     /translation="MSDENKSRRCSFELFPDERTGDKIADELIANEKLKERGRFMRAM
                     LVTGAAFAAIDKRLPLLISELLTENTTLDDINKVISSVIPGAFSVEKKLLELLEKQSG
                     LHTSVDCSTPLTEQSLSRNDGEDQTRRNAENMFGDD"
     gene            complement(9092..10366)
                     /locus_tag="ACHKFK_002365"
     CDS             complement(9092..10366)
                     /locus_tag="ACHKFK_002365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO9052690.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEFFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSQCYTTKWRDIPLARLG"
     gene            complement(10366..10803)
                     /gene="umuD"
                     /locus_tag="ACHKFK_002366"
     CDS             complement(10366..10803)
                     /gene="umuD"
                     /locus_tag="ACHKFK_002366"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9052691.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(10800..11048)
                     /locus_tag="ACHKFK_002367"
     CDS             complement(10800..11048)
                     /locus_tag="ACHKFK_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9052692.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            11466..12368
                     /locus_tag="ACHKFK_002368"
     CDS             11466..12368
                     /locus_tag="ACHKFK_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO9052693.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTVAGRGVVSTENIAFTRWLTHLQNGVLLDKQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVLSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            12365..12676
                     /locus_tag="ACHKFK_002369"
     CDS             12365..12676
                     /locus_tag="ACHKFK_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052694.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSLLASVTVYASLSPFSDERPSSRFRGIALPSERRRLRYSTVGLTRYRT
                     R"
     gene            12753..13436
                     /locus_tag="ACHKFK_002370"
     CDS             12753..13436
                     /locus_tag="ACHKFK_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO9052695.1"
                     /translation="MSRFVLGNCIDVIARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            13437..13658
                     /locus_tag="ACHKFK_002371"
     CDS             13437..13658
                     /locus_tag="ACHKFK_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052696.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            13588..14106
                     /locus_tag="ACHKFK_002372"
     CDS             13588..14106
                     /locus_tag="ACHKFK_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO9052697.1"
                     /translation="MAPIACLWRRIPKFWNWIPASKIEKETRMYGTCETLCRELAAQY
                     PGDAPLMLVVWSPEEIQALADGMDISLSDHEIRTVLARLEDIPEDQRIESGISSAAAM
                     EIISNVSENRQVTVPAELLASLIQTAEQALWKREWAARDHGLAVPECVTRRQAVVNQA
                     RTLLKNNTREND"
     gene            14151..14765
                     /locus_tag="ACHKFK_002373"
     CDS             14151..14765
                     /locus_tag="ACHKFK_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052698.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVVLSGSQWQSLPVLPAD
                     GTQVSFPYAGEWLTEEEIRAVFDAMRDAVCSVSCRVAEDARRIRAALTTTGQTLLTRQ
                     TRRFRLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSSVEMYLVSECVEHILSSGL
                     ACDVLRIPDEPSRRWFDRDILREVVLEARTEIRSMADALAKIRK"
     gene            15180..15605
                     /locus_tag="ACHKFK_002374"
     CDS             15180..15605
                     /locus_tag="ACHKFK_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9052699.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            15652..16074
                     /locus_tag="ACHKFK_002375"
     CDS             15652..16074
                     /locus_tag="ACHKFK_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO9052700.1"
                     /translation="MYCTVKEIIRDVLDTDVPDSECVFAVVLTRGDVRYIAQDWNLSD
                     DELETVMQRLDDAFEHGADVSVVHDVVRELMEEKHASRQVTVPAVMLEKVMALAGSEI
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            16071..16262
                     /locus_tag="ACHKFK_002376"
     CDS             16071..16262
                     /locus_tag="ACHKFK_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052701.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            16415..16648
                     /locus_tag="ACHKFK_002377"
     CDS             16415..16648
                     /locus_tag="ACHKFK_002377"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052702.1"
                     /translation="MIFRDTVTVSGCLLLRPLRLPAAWRHPVVRPDSDGQLLAHPPVS
                     AEPAGAVSGCRYGPMRENPACSDRSGRAAPVAP"
     gene            17032..17559
                     /locus_tag="ACHKFK_002378"
     CDS             17032..17559
                     /locus_tag="ACHKFK_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9052703.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGKLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     TPEGEDYGFSDDIPF"
     gene            17617..17850
                     /locus_tag="ACHKFK_002379"
     CDS             17617..17850
                     /locus_tag="ACHKFK_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HFO9052704.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRNNNGLVWRTWNFEDGAGYWMNHVIRNFGILK"
     gene            17909..19867
                     /locus_tag="ACHKFK_002380"
     CDS             17909..19867
                     /locus_tag="ACHKFK_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO9052705.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMARYDALENQCEESDLLEAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CVQRGVQLRSEDDATDDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            19922..20356
                     /gene="psiB"
                     /locus_tag="ACHKFK_002381"
     CDS             19922..20356
                     /gene="psiB"
                     /locus_tag="ACHKFK_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO9052706.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            20353..21072
                     /locus_tag="ACHKFK_002382"
     CDS             20353..21072
                     /locus_tag="ACHKFK_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO9052707.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            21069..21665
                     /locus_tag="ACHKFK_002383"
     CDS             21069..21665
                     /locus_tag="ACHKFK_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052708.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIGQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            22127..22627
                     /locus_tag="ACHKFK_002384"
     CDS             22127..22627
                     /locus_tag="ACHKFK_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO9052709.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(22843..23069)
                     /locus_tag="ACHKFK_002385"
                     /pseudo
     CDS             complement(22843..23069)
                     /locus_tag="ACHKFK_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422627.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23359..23793
                     /locus_tag="ACHKFK_002386"
     CDS             23359..23793
                     /locus_tag="ACHKFK_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO9052710.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            23885..24151
                     /locus_tag="ACHKFK_002387"
     CDS             23885..24151
                     /locus_tag="ACHKFK_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052711.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            24328..24717
                     /locus_tag="ACHKFK_002388"
     CDS             24328..24717
                     /locus_tag="ACHKFK_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HFO9052712.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            24714..25604
                     /locus_tag="ACHKFK_002389"
     CDS             24714..25604
                     /locus_tag="ACHKFK_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9052713.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLGCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSDDELKKITH"
     gene            25604..25849
                     /locus_tag="ACHKFK_002390"
     CDS             25604..25849
                     /locus_tag="ACHKFK_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052714.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPPERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(25880..26131)
                     /locus_tag="ACHKFK_002391"
     CDS             complement(25880..26131)
                     /locus_tag="ACHKFK_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO9052715.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            26712..27560
                     /locus_tag="ACHKFK_002392"
     CDS             26712..27560
                     /locus_tag="ACHKFK_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO9052716.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKRIIIENLLYRWSNGRMTLDCSGREALDDLVRPFYLLEERNVPDFRS
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            complement(27646..27981)
                     /locus_tag="ACHKFK_002393"
     CDS             complement(27646..27981)
                     /locus_tag="ACHKFK_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO9052717.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            28214..28546
                     /gene="mobA"
                     /locus_tag="ACHKFK_002394"
     CDS             28214..28546
                     /gene="mobA"
                     /locus_tag="ACHKFK_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO9052718.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            28558..31269
                     /locus_tag="ACHKFK_002395"
     CDS             28558..31269
                     /locus_tag="ACHKFK_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO9052719.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            31337..31768
                     /locus_tag="ACHKFK_002396"
     CDS             31337..31768
                     /locus_tag="ACHKFK_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO9052720.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            32118..32522
                     /locus_tag="ACHKFK_002397"
                     /pseudo
     CDS             32118..32522
                     /locus_tag="ACHKFK_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(32629..>32992)
                     /locus_tag="ACHKFK_002398"
     CDS             complement(32629..>32992)
                     /locus_tag="ACHKFK_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFO9052721.1"
                     /translation="MMSNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAA
                     NMFYDPADRDDLCLDPRRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEE
                     QRDLAIAAAIKQVRQTSY"
BASE COUNT         7669 a         8231 c         9265 g         7827 t
ORIGIN      
        1 cccctcaaag ccatatcacg tacgttaatc ctgacttcat taaaatcagt ggtttcactg
       61 aggaagaact attaggccag cctcacaaca tcgtaagaca cccagatatg ccgcctgctg
      121 catttgagca tatgtggagt acattaaaat ctggccgctc atggatgggg ctagtaaaaa
      181 atcgctgtaa aaatggcgac cactattggg taagtgctta tgtaacgcca atagctaaga
      241 atggttcgat tgttgaatac cagtctgtaa ggaccaagcc tgaacctgag caggttttgg
      301 ctgcggaaaa attatatgct caattgagaa gcgggaaggc cgcgaggccg aaattggctg
      361 ctagcttttc cgtgaaaata ctcttgctca tatggggtag tattatatca agcgcaatgg
      421 ctgccggcat gcttactgat acatcaataa gcagcttatt gttagccact ttaatgtcag
      481 gaagcttaag ctctgttagt gttttggcta ttctctctcc tcttggaaga ctggttgaaa
      541 gagccaggaa tatttccaat aacccattaa gtcaatccct ctacactggg cgcaccgatg
      601 agtttggcca aatagagttt gctttacgaa tgatgcaagc tgaaacaggc gccatagtag
      661 gtcgcatagg tgatgcatca aatcggctta gcgaacacac ccgaggccta ctaaaggata
      721 ttgagtcaag caatgtactt acagttgagc agcaggcaga gacagatcaa atagcaacgg
      781 cagtaaacca aatggtggca agcattcaag aggttgcgag caatgcacag catgctgcag
      841 atgcggccgg aagagcagac actgagacgg catctggcca gcgtctggta gcccacacaa
      901 gccagtcaat cactgccctt gaaggtgaaa ttaggcaagc cactcaggtt attcatgagc
      961 ttgaaggtca aagtaacgag atatcaaaag ttcttgacgt tatacgaggg atcgccgagc
     1021 aaacgaattt gttggcactc aacgcagcaa ttgaggccgc gcgtgctggt gagcaggggc
     1081 gtggttttgc tgttgtcgcc gatgaggttc gcagtcttgc tgctcgcaca cagcaatcga
     1141 caacggatat tcaaagcatg atcagcgctc tacaagagcg agcgcaatcc gctgttacag
     1201 tcatggagca aagtagtcgg caagcgcaca cgagtgtagc tcacgcagag gaagcagcta
     1261 cagctcttga tggaattggc caacgcgtta acgaaattac cgacatgaac gcgcaaatag
     1321 cgactgcggt cgagcagcag ggagcagtaa gtgaagacat aaaccgcagt attatcaata
     1381 tacgcgatgc tgctgatacc aatgtacaga ccgggcagaa taatttgcaa agtgcgaaat
     1441 ctgtcgctca gttaactagc gctctgagcg aactggcaaa acagttttgg gaaaaacgag
     1501 gataacgctt ttcagtatct cgacagggat aacttacttt accatcggtt atccctttta
     1561 gccgcacaaa ttttagccat ggcttttcag gaagtcaggg ctatcagaat ggccttagaa
     1621 agcctagtca aagagctgtc acgagaacac cgttagctta gcgtacgatt ttttccgaat
     1681 tctgcggttc ccccatcctg cacgctccat ctgataatgt tctgacaggt aaaaatcata
     1741 tcctgcatga tttgtgtgct cctgatttct gataatatca ggtatggttt tttcatcaat
     1801 gatgaaaatc atatctgata tgattttgtg tgtgtttttt tattatcata ttatttgcgt
     1861 cttttctgcc gcgcagatcc ggtttccggc gaattcggaa atataagtca ggttacgtct
     1921 ggtacacttc ctgagtagag tattatctcg gagaaagcct tttatgaatg tggaagagtc
     1981 agagcagcgc tgggtttgtg cttgttgtat tggcgaagaa tttttacgcc acaaggtcga
     2041 gaaagaagga cgtatacaaa cctgtgatta ctgcgatgag attcacacct gtttttccct
     2101 ggaggaggta tgcgacctga cagaaaaggc tattgaagct catttttacc ggactgacac
     2161 tgaaccgaac gatatggagt atgccagtct tcgtcatatt gacggttatg agtggttccg
     2221 tgagggggag aatgtagtgc agctcattga agatttgctc cagtctcgtc gtgcactcgc
     2281 agacgatatt cagcagctgc tcgaatacag acattcagat tttgattccg atgtgatggg
     2341 acttgagacc gagtttgccc gcgagtcctg ttacgcagaa cgcaaacaga tcagtaccgg
     2401 ccgtctcgat tccatgtgga taaattttgt tacctccctg aaaacagaat cacgcttcat
     2461 caataatgcc gtgagctcca ccctggacgg tatattccag ggggtagaga caatgcgcgc
     2521 ccgtggaaaa gaacctgtga tcattgacgc tggaccaggt acagctatcc cgtcactgta
     2581 ccgtgcccgg tggtgccgga atcatcatga gctggagcaa atacttgtga cgccggacaa
     2641 agaattaggt ccgccgccgc atcagttcag cggctctaac cgtatgagtg caaagggcat
     2701 ttcggtattc tatggtgcca gttcagtcga tacggctata tctgagatac gtccaccggt
     2761 aggctgcaaa gtggtgtgcg cgaagttcag tcttatccgg tcgctccgtc tcctgaacct
     2821 gccggcgctt gaaagtgttc tggagagcgg aagtctgctt gatccggact acataaaaag
     2881 acgtgagcag gccgcattcc tgtccacatt taccagccgg attgtcgagc cggttctgcc
     2941 tggcgaagaa gatttcagct atataccaac tcaggtcatc gctgaatacc tggcagatcc
     3001 tctccgcttt aatcttgacg gtatcctgta tccgtccgtg cagttgccgg gggcggatca
     3061 ggaggaacgg tacaacgttg tgttgttcca taaagcctcc cgtgtcgcct ttatgacgct
     3121 accggcaaag gatgactgca tgatcagata tggccatcag tatgccgaag acgaatggga
     3181 gactgatatc tgtgtgacag aggttatctc tgctcaggaa gaccctcctc cagccaggca
     3241 tgatctgtct gacctttcac ttcccgacga cccacgggag cctgctctgg agatagatct
     3301 cgattctgtc agcgttcacg gtatacgggc agcccggttt gaatactcca ctgatgccgt
     3361 caggcggagc acataccact atactccgcg gacttcagaa ccagccggta ctataactca
     3421 gttatgggac accgcctctg gtcatgacga ttttccgttc tgatgcagaa tcaagccgct
     3481 ctcattttca gatcatgtgg aactgacggc tcttcaccat ctgttcgagt ccgttaagat
     3541 cgaactgatg gttactgcgg atattctggt acacctcaag catgacggcg tacttatctg
     3601 tcagatcctt gtcattacgc agaccgtaat cgccgatgcg gtgattacct tgtccgttat
     3661 caatccagtt aagcagcgac cacatgtcaa tctgcagcgc atagtcttct gtgtcaggat
     3721 accccttgaa agttaactgc agtccgttgt cttttacata gccctgaatg cgggccagcg
     3781 cgtcgcgggt cttctggtca ttccaatcgt tcactgtttt ctcggcctcg gcaagtacca
     3841 tgctgatatt cgagaacatg tcacgaagcg ctatcacgct gtccagggta ttacagatca
     3901 ccggactggg ctgcggattc atcagggata caatcatgtc ctggcccagg ttagggttaa
     3961 tggcttgcag gtgcacggca tcgagctcaa agaaccatgt catgcgttgc agataaacct
     4021 gacgcaccag agcttctgct gtgacagtac tggcgacacg attgttatag ccataagtgg
     4081 ggttgatagg ctggacagaa cgtccgtctt taccgcactc catacgcagc aactggcaga
     4141 gctggaaaaa gatgttcagg cgcgctgaca ggggaaacat ctccgcttcc tggctggccg
     4201 gagtaaattt cccgcgaaca ccctgcgcta taaacgaacg cagaacatcc gagcgggacg
     4261 gcttatactg accgtcaatg ttgttaatga aattatccag ctcatcaagt tgctgctgag
     4321 gaagacggag ttcaatacgt tggtctttca tgtccacggg ggcctcactt ggtttcgttt
     4381 atctggtttt attacattcg cgtacgttca caaactgtaa atgtacgggt taaacccgta
     4441 cgcgtccagt aagaatgcgg ttttattttg tctgtttcca ctgagatccg aaaactcatt
     4501 gttacggatc ttcctctccc tccgcacagc gccatctccc gtcagcacaa catgtagtgc
     4561 tgaattcagc tgctcttgac actatatgtt gtgtcatctc cctgacctgt gatgcgccgc
     4621 gcaggggcgg aaaacagcga tatgatgatt ttctcagcgt ggtacacttc cggaaagttg
     4681 tggtattccg gaaagtcgga tctgacggaa acggctctcc ggtaatttaa cggcgtggtt
     4741 atatggatgc ttgttatcat ggtgatgatg ataacggcat gatgttatca gacggcgtga
     4801 cggtaagggc agtgatgatg gatgacgtta tcgcatgacc gtccctgccc ggaaaagaaa
     4861 aaaggagtca cccatgtttt ttattgagaa tgaaggtcag gctgtcgccg gaacggatta
     4921 ctggcagtct gtacaggcgc aggccggata tgtctacctc agctggaatg ccggcgcagc
     4981 caggctgctt gtcccggatg cggcaaaaca tttactcagg gagatgcggg gggctgagta
     5041 cgtcatcatc agtaagggag cactgcatgg ccgcgatgcg ctggaactgg tatttgaaga
     5101 cggcagcgat gcgccgtttg tgatccacat gctgagtgag cagtgcgatc gcctgctccc
     5161 cgaaaacaac cagggagggg gttttgttgt caccgtctgg acgcgtggag gtaaccagct
     5221 ccgttatccg ggaaagtacc gtgttgtgga aaacctgccc gacgtttccc cgtggagtga
     5281 acactgatat gcagcacctg ccggcaccga tccaccatgc ccgggatgct gctcagcttc
     5341 ctgttgccat cgattatccg gcagcgctgg cactccgcca gatgtcgatg gttcatgatg
     5401 aactgccaaa atacctgctg gcccctgaag tgagcgccct gctccattac gtgccggatc
     5461 tgcgccgcaa aatgctgctg gccacactgt ggaacaccgg tgcgcgcatt aatgaagcac
     5521 tggcgctgac gcggggggat ttttcgcttg cgcccccgta tccgtttgtg cagctggcca
     5581 ctctgaagca gcggacagaa aaagccgcca ggacggcagg aaggatgccc gtcggtcagc
     5641 agactcaccg tctggttccg ctctccgacg cctggtacgt cagccagctg cagacgatgg
     5701 tggccacact gaaaatcccc atggaacggc gtaataaacg aacaggcagg acagagaaag
     5761 cgcggatctg ggaagtgacg gacagaacgg tcaggacctg gattggggag gcggttgccg
     5821 ccgctgccgc tgatggtgtg acgttctctg tcccggtcac gccacatacg ttccgccatt
     5881 cctatgcgat gcacatgctg tatgccggta taccgctgaa ggtcctgcag agcctgatgg
     5941 ggcataagtc catcagctca acggagatct acacgaaggt gtttgcactg gatgtggctg
     6001 cacggcaccg ggtgcagttt tcgatgcctg agtccgatgc ggtgtctatg ctcaaaagaa
     6061 ttccataagc tctgactttt ttaaatccct ttcggatatc tcatggatat attatcggta
     6121 tttcatattc tgttgacatg caggaccggc atagagtaaa aaaacatcta tttagtacct
     6181 cttggatatc cgaaaggtgt cttatggggt ctttatgggt attacatgtg gattctggag
     6241 atacactatg cctgttattg ctaatacgca tcccaaaggt ggtgtgggta agacaacttc
     6301 atccgtaaac attgttggtg aaatgaagtc tgatacagtt gatctggata ctcataccgg
     6361 gctttctatc attctggggc tgagacctga aggaaaagaa atttccgtga aagtacccaa
     6421 aacagtggat gagttaatcg aaattatgac tccctacaag aacagcgata agacgttact
     6481 tattgactgt gggggatttg actcagatct tacccgtact gcgattgcct ttgcagactg
     6541 cgttattgtc ccttcaaaag actccctgac tgaacgtatt ggtttgatgc attttgatgg
     6601 agtactggac gaaatcagct ctatcatggg gaccgatata actgctcacc tgtatctctg
     6661 taaagttaat cctaacaaga agaaattccc taagcttgat gcaatcctgc catcgttcaa
     6721 acatctgaag ctgatgaaga gccgcatttc tgctcgtgca gaatttgagg atgtcattga
     6781 gactgggatg ggaatcactg agtctgttca tgggcgctat tcggcaggag gtaaggaagt
     6841 tattgccctg atcgaagaaa tcaatcatct gattgaaaat aacaaacagt aaggtgtgtc
     6901 tattgggtat ccatatagta tcctttgggc ttttaatgga tatcttttaa gtatctaaca
     6961 aacatcctgg gttatctcat gagcaaagcg aaatttagtg cacaggctga aaaactgaaa
     7021 aacctggtgg attctgccag attacaaact tcttcttctg ccgtaacagc aacagccccg
     7081 tctcagccaa atgaaggtgc agaggaagtt tcaggtcgtc ccgtggctgc caggacgaag
     7141 gctaagaatc ttaaggccat accgctgtcc tattttgaag ctcatgcgga acttaaagcg
     7201 accagtaaaa catctctgga ttttagctca tatattattg aggctataag agagaagctt
     7261 gaacgtgatg gtgcaatagg tcaataaact atcggatacc ttaagggtat ccgatgtgtg
     7321 tcttttaaat gcgttatggg gattcttcac gtacctaaag agtccccatt agacatcttt
     7381 ctgtttatgg tgacatgatt cgtcaccgct ttcctttcct ctcagtttca ccatttcatt
     7441 ccttttcccc taacctgaac catgatgtcc acgatgtttt tatattgcat ttatgtatca
     7501 cgtataacct tcataatgtt tttgtatcta tgatttcctc tggtcttaaa gttgtgtgta
     7561 caatatacat gcaatttatc gtgtttttat gttgcattta tcctttcaga aagaccgttt
     7621 tttcattata attgaatata cattgcatgt atgaagcatg tttgtttttt ctatttgttt
     7681 atgagcaacg gagactggtt atgaaaatct ttattgatga tggttctacc aacattaaac
     7741 ttgcctggtt ggaggatggt gacgtgaaaa cgttaatcag ccctaacagc tttaaaccgg
     7801 aatggtcttt tagtttactg gatgatgctg ccccggctaa ttacgagatc gacggcgaga
     7861 agttttcatt tgatccatta agtgctgatg ctgtcgtaac gactgaaaca cgctatcagt
     7921 acagtgatgt gaatgttgtg gccattcagc atgcgctaca gcagaccggc ctgaaagcgc
     7981 agcctgttga tgtcatcgtc acattgccga tctctgaata ccttgacgcc aacaatcaga
     8041 aaaataagca aaacattgaa agaaagaaaa aaaatgtgat gcgtcaagta cgggtgcagg
     8101 ggagtgatgc atttgttatt cgcagtgttt ccgttcttcc tgaaagtatt cccgctggtt
     8161 tcagtgttct tgcggggctt gaggatgatg aatccctttt gatcgtagat ctcggtggta
     8221 caactcttga tgtgtcgcat gttcgtagca aaatgacagg gattacaaaa acctggtgtg
     8281 atccaaatat cggcgtgtcc ctgattacat ccggggttaa ggaacagatg gctgtacatg
     8341 ctaatacaag agtgagctca ttccaggctg acaatatcat tgtgcaccgt aatgaacctg
     8401 actacctttc ccgtcgcatt tataatgctg aacagcgtga gtctatcatt aatgttatca
     8461 atgaacggca gaagttgctt attaaacgag ttaatgatgt catcagccgt ttcaccgact
     8521 atacacatgt aatgtgtgtt ggtggtgggg ctgaaattgt ggccgaggct gtgaagaacc
     8581 tgactaaggt tcctgatgag cgcttttatc tgagctcatc cccgcagttt gatttagtca
     8641 tgggaatgat aaaaatgaaa ggtggtgtga ctaatgagtg atgaaaataa atccagacgc
     8701 tgctcatttg aacttttccc ggatgaacgg acaggagata agatagcaga tgagctcatt
     8761 gcaaatgaaa aactgaagga gcggggacgt ttcatgcgcg cgatgttagt tactggtgcg
     8821 gcgtttgcag ctatagataa acgtcttcct ttgctgatat cggagttact gacagaaaat
     8881 acgactctgg atgatattaa taaggtgatt tccagcgtga taccaggtgc tttttcggtt
     8941 gaaaagaaat tgcttgaatt actggaaaaa caatctggtc tccatacgtc tgtggattgc
     9001 agtactcctc ttacagagca gagtctgtcg agaaatgacg gtgaagacca gaccagaaga
     9061 aacgctgaaa atatgttcgg agatgactga actaacccag cctggccagg ggaatatctc
     9121 gccatttagt tgtataacac tgactcaaca tttcccgttt catttgccat tcaggggcgg
     9181 ttcctcgccc cgcaaaccac actttccctt ttcccgactg gttcagttca tcgagtgttt
     9241 tcattaactt ttcactgttt ttacggggct ggatttcatc aaataatccc ggctgcgcta
     9301 tacccgatgg tgtgaaatca gccagcatga ctcctgcctt catatagcgg tatccttcac
     9361 gccagacatg gtttaaggct ctgcatgcgg cggcaataat gtcccggctg tcctgtgtgg
     9421 gcaatggaag cttttccaca gcggcattgc tgtaacaggg ttcttttact gcaaaggggg
     9481 atgtccgtac aaatgtcgtc acctgccggc aatactgacg ctccccacgt agtttctctg
     9541 cggcccgctc tgcatactga acaacagcct ggtgcatggc atctttgtct gtgattcgtt
     9601 caccaaaact gcgactacag acaatctgct gttttgccgg tggtgcttct tccagggata
     9661 tgcaggactc gccgttgagt tcgcgtaccg tacgctcaag aatgacgctg aagtttttcc
     9721 ggatgaatgc cgtgttagcc tgcgccagct gcagtgctgt gttaataccc agcgcattca
     9781 gcttttccgt cagtctgcgt cctactcccc agacctcacc aactggctgc agtcccagta
     9841 gcttcaagat ccgattacgg ttttctgccg tcagcgcgac cacaccggaa aactgtggcc
     9901 attgctttgt tgcccactgt gcacttttag ccagcgtttt tgtaggcgca atgcccaccc
     9961 ccatggtgag tcctgtccag ctctttacct gttccctgag ctgatgacca aaaaactccg
    10021 gagagatgca atgatttatc ccccgcaaat caataaacat ttcatcaatt gagtagggct
    10081 caactgcggg agaaagcgac tccagaacag ccatgacccg ttggctcatg ctgtggtaca
    10141 gcgcataatt gctggaaaat acatgtattt tcttctccag gcgcatctgt ctcacctgaa
    10201 accagggctg ccccattctg atgccaagag cttttgcctc cgggctgcgc gcgatcacac
    10261 agccatcgtt attgctgagt acgatgaccg gttcgttgcg aaggtccggg cggaaaactt
    10321 tttcacatga ggcgtagaaa ctgttgatat cagccagtgc aaacatcagc gtaactcccg
    10381 cgtcctgtgt atcacgtgag tgacaacacc aaaaatacag atgttttccg gatacagtgt
    10441 gcggaactcc gggctgtctg aaaccggctc cagtgccggg cgtgggcgca acagcagtcg
    10501 tttgacggtg aactcaccgt cgatctcagc gataacgatg tccccgtgtt gtggtttttc
    10561 ggccctgtcc actaccagca gatcaccatt ctgcacgcca gcctggttca tcgattcacc
    10621 gctggcgcgc agaaagaagg tggctgcagg tctgctgatg caatagctgt tcagatccag
    10681 ttcctgctca gcataatcag tggcaggcga aggaaaaccg gcctggcaac gatcggcaaa
    10741 caacggtcgc acgtaactgt catcgcctga agggtcagcc ggacggtgat aaacggtact
    10801 catgctgcag gcagccagct gtcgtcctgc cagatttctt ccagcagaga caggatacgt
    10861 tctttatcct tgtcgttgcg ggctcctgtg acagatacag acggggcgct gccctggtct
    10921 atacgcagcc agatttccgg gtagtgtggt ttcagacgtt gttcgatttc attctgtagc
    10981 gcctgaagtg tgcctgattt gaggtttttt gtgctctggc ggtcgaaaag aatttcaatg
    11041 cggatcatgt tctcatctcc cttttctatt gctgtatgca tatacagtat tctaataact
    11101 gaatccacac acagtcaaat gtcattattg tgactaaaaa acgctcaact ctctgagagt
    11161 gtggctgtat gcctgctgtg gactgctgtg tccagcttat ccacagcatt ttgcgcacgg
    11221 ttctgtgaat aaaatacctg gttacccagg ccgtgccggc acgttaaccg ggctgcatcc
    11281 gatgcaagtg tgtcgctgtc gccggcctcc tcacccggtc acgtttcgtc gttcctcctc
    11341 cacgagctgc ggcttcgggg ccgcacctgc attcgtatgc ggttgcccgg ttacaggtgc
    11401 ggcacggcct gatggaggcc gcatgtgaga ggagaattcc catgccaaac tggtgctcaa
    11461 atcgtatgca tttttctggt gaaccggcac agattgctga gattaaacga ctggccagcg
    11521 gtgcagtcac accgttttat cgccgcgcca caaatgaagg tattcagctg tttctggccg
    11581 gaagtgccgg acttctgcag accactgaag atgtgcggtt tgaaccatgt cccggactga
    11641 cggttgccgg acgtggagtt gtatcgacgg aaaatatcgc gttcacccgc tggctgacac
    11701 acctgcagaa cggtgtactg ctggataagc aaaactgcct gatgctacat gaactctggc
    11761 tgcagagcgg tactggacag cgtcgctggg aaggattacc ggacgatgtc agggatacca
    11821 tcaccgcact tttcaccgca aaaagaggtg actggtgtgg cttctggagt aacgaggatg
    11881 tatcggtgtg gtggaaccgt ctgtgtgaca acgtactgcc ggaaaaaacc atgccgtttg
    11941 acctgctgac ggttctgccc acccgcctgg atgttgaagt gaatggcttt aacggtggtg
    12001 ttctgaacgg tgttctttct gcatatcact ggtatacgga acagtatggc gtgaagtggc
    12061 ctgtggggta tgaggtgaat atcagcagtc agggagacaa cttcattcag gttgatttcg
    12121 acactccgtg gtgtcagccg gaaagcgacg ttattgcaga attaagccgc cgtttcagct
    12181 gcacgctgga gcactggtat gccgaacagg gctgtgattt ctgtggctgg cagttgtatg
    12241 agcgcggaga gctcgttgat gtgctgtggg gggaactgga atggtcttcc ccgacagatg
    12301 acgatgagct gccggaagtc accggacctg cgtggatagt cgacaatgtg gcgcattatg
    12361 gcggatgaag tatgacggag acgggcgggc aaccgcccgt ttcttttccg gtaaaggatg
    12421 tcgccgtgct cctcttttta ctgcgccggc tgacgcggcg cggcataaac gctgcctgtg
    12481 gtcagtgtcc cgcgtccggt gggcacggga cgggcgcttt taccgctccc tgctggcgtc
    12541 ggtgacagtg tacgccagcc tgtcgccctt ttctgatgaa cgcccttcaa gccgctttcg
    12601 cggcatagcc ttgccgtcag aaagacgacg gctgcggtat tccacggtcg gcctgacccg
    12661 ttaccggacg cggtgaacag cccacaggca gcggggaacg ggcaccacag gggtgccctc
    12721 ccggtgtcct ctgtaagaga aggagcgttg ttatgtcccg ttttgtcctc ggtaactgca
    12781 tcgatgttat agcccgtatt cctgataacg ccattgattt catactgact gacccgccgt
    12841 atctcgtcgg tttccgtgac cgttccgggc gcaccatcgc cggcgataaa accgatgaat
    12901 ggctgcaacc ggcctgtaat gaaatgtacc gcgtgctgaa aaaagatgcg ctgatggtca
    12961 gcttctacgg ctggaaccgc gtcgatcgct ttatggccgc ctggaaaaat gcgggattca
    13021 gcgtggttgg ccacctggtg tttaccaaaa cctacacatc gaaggccgca tatgtgggct
    13081 atcgccacga atgcgcctac atcctggcaa aaggccgtcc ggcgctgcct cagaagccat
    13141 tgcccgacgt gctgggctgg aaatattcag gcaatcgcca tcacccgacg gaaaagcccg
    13201 ttaccagcct gcaaccgctc attgagagct tcacacaccc gaacgcgatt gtgctggacc
    13261 cgtttgcagg cagcggctca acctgcgtgg ccgccctcca gtccggacgc cggtatatcg
    13321 gtatcgagct gcttgaacag tatcaccgtg ccggacagca acgactggcc gccgtgcaac
    13381 gggccatgca gcagggggcc gcgaatgata actggtttga accggaggct gcgtaaatga
    13441 actatgccgg acacgaaaaa cttcgcgccg acgtggcgga ggtggccaac accatgtgtg
    13501 accttcgggc caggctgaac gatatggagc atcgctgtcg ctttgattcc gatgtgcttg
    13561 ttgaacgcct gacacgccag acgctgtatc gcgccaatcg cctgtttatg gaggcgtata
    13621 ccgaaattct ggaactggat tcctgcttca aagattgaga aggagacgag aatgtacgga
    13681 acatgcgaaa cgctctgccg ggagctggca gcgcagtatc cgggagacgc gccgctgatg
    13741 ctggttgtct ggtccccgga agagatccag gccctcgctg acggaatgga tatttccctg
    13801 tccgatcatg aaatcaggac cgtcctggcg cgcctggagg acatcccgga agaccagcga
    13861 attgagtccg gtatttcttc cgccgcggcg atggagatta tcagcaacgt gagcgaaaac
    13921 cgccaggtga ccgttccggc tgaactgctg gcgtccctga ttcagaccgc tgaacaggca
    13981 ttgtggaaac gtgaatgggc cgcccgggat catggcctcg ccgtcccgga atgcgtcacc
    14041 cgccgtcagg cggtggttaa tcaggcccgc accctgctga aaaacaacac acgcgaaaac
    14101 gactgatgtt atcgccgcct gcgggcggcg attcagggag accatgaatc atgaacgaaa
    14161 cgctgaatgc actgatttgt cgccacgccc gtaacctgct gctggcgcag ggctggccgg
    14221 aagagacgga tgttgtactg tccggcagtc agtggcagtc gctgccggta cttccggcag
    14281 acggaacgca ggtgtctttc ccgtatgccg gtgaatggct gacggaggaa gaaatcaggg
    14341 cggtttttga tgcgatgcgc gatgcagtat gcagtgtcag ttgccgggtg gcagaagatg
    14401 cgcggcgtat ccgtgcggcg ttgactacca ccggtcagac gttgctgacc cgccagacgc
    14461 gccgctttcg tctggttgtg aaggaaagcg atcacccctg ctggctcgat gaagacgacg
    14521 aaaacctgcc cgtggtgctc gatgccatcc tgaaccgggg cgctcgtttt tcgtcggtgg
    14581 aaatgtatct ggtcagtgag tgtgtggagc atatcctgtc cagtgggctg gcctgcgatg
    14641 tgctgcgtat accggatgaa ccgtcccgtc gctggtttga ccgggatatt ctgcgggagg
    14701 tggtcctgga agcccggact gaaatccgca gcatggcgga tgcactggcg aaaatccgga
    14761 aatgatgacg ctggcgggga aaaatctccg ccttttccag ccggtccccg gaaagagcaa
    14821 aaggatgtcg ccgcaggctc cgctttaccc ggccatgcgg ggcatggcct tgtgggtttt
    14881 cagttatgtg gcctcagcgc cgtgtgcggg ctgtgccgtg cctccatcgt agccgggctt
    14941 ctgcgggcgg gttgcaaggt aagatgaacc gctgcggtca cccggtccct ccttcccgtc
    15001 tgccgtgatt ttccggtcgt tcacccgctc agatttcgcg gtctcaccct ccaactcccg
    15061 ccagccgtgt gcgtcaggct gcggcttccc ttgcgccctt gcaaccctgc aggcccggca
    15121 aagatggagg cacggcaccg cccggtgtcg cggaatatgt gaacaacgga ggaaatccca
    15181 tgcagtatgc gaaacctgtc actctgaatg ttgaagagtg cgaccgtctg ttttttctgc
    15241 cttacctgtt tggccaggat tttctgtatg tcgaagcgtc tgtgtgcgcg ctggcgaaaa
    15301 aaatgatgcc ggaatatgaa ggcggattct ggcacttcat ccgcctgccg gacggtggcg
    15361 gttacatgat gccggatggc gaccgttttc atctggtcaa cggtgaaaac tggtttgacc
    15421 gtaccgtgag tgctgatgcc gcaggcatca ttttgacttc ccttgtgatt aaccgccagt
    15481 tgtggctgta ccacgacagc ggggatgcag gactgaccca tctttaccgg atgcgcgatg
    15541 cgcagttgtg gcgtcacatc gaatttcacc ccgaatgcaa cgcgatttac gcagctctgg
    15601 actgattaac ggacggggcg gcaatgccgc ccctgtaaag gagacgagaa catgtactgt
    15661 actgttaaag aaatcatccg cgatgtactg gatacagacg tgccggacag tgaatgcgtt
    15721 tttgccgtgg tgctgacccg tggggatgtg cgctacatag ctcaggactg gaacctgtca
    15781 gacgatgagc tggaaaccgt catgcagcgg ctggacgatg cctttgagca tggtgcggat
    15841 gtcagcgttg ttcacgacgt tgttcgtgaa ctgatggagg aaaagcacgc cagccgtcag
    15901 gtgacagtcc cggcggtgat gctggaaaaa gtgatggcgc tggcaggcag tgaaataaag
    15961 cgcctgtatg ccgtcgggag tgagaacggg ggcgacggtg atgcattcgt cagggaagaa
    16021 cgcgaagcaa tggacgttgt gttacaggcg ctggacgggg agcacatgtc atgaatatca
    16081 gcacagaaac ccgcgaaatt ctgcgcaatt acaaagccgt gattaatgcg cggcgtcgtg
    16141 aaatggggca gaaaccgctc accactgcgc agattgttga tgaaatctgc gattttgtgg
    16201 cgaatcagca ggcggttttc ctgggcggtc actacatcct tcagggcagc agaaacaggt
    16261 gatacagggg caggcggtgg aggccgcctg tcagcccgcg cgaaaacccc tggcaggctg
    16321 acgcctgccg gaaacgcggg gtgcagcgcc tgcggcgctg ccggcccggc tttccggtca
    16381 gaaggtagtg tatggctgta tgcagggaac aggagtgata ttccgtgaca ctgtcaccgt
    16441 ttctggctgt cttttattgc gcccacttcg gctcccggct gcctggcggc atccggtcgt
    16501 caggcccgac tccgacgggc agctgctggc gcacccgcca gtctccgccg aacccgctgg
    16561 cgcggtttcg ggctgccgct acggtccgat gcgtgaaaac ccggcttgca gcgaccgttc
    16621 cggtcgcgcc gcccctgtcg cgccgtgacc tgcgctccct gcacacctga cggtgtgtga
    16681 ggaaactgcg cctgatggcg cagcagtcct ccctcctgcc gccactcctg cggcctgttt
    16741 cccgctgtgg actttccggg gggcagacgt aaccccctcc cgttccatct gtccagggtg
    16801 agatgcaccg gccacaaaat ttttagccat gaacccggca aaaaattttg cgtccgcccc
    16861 cggacagctg ccccgtgagg cggtaaacgg gcccccatcg gaaaggtcca ctgcggggag
    16921 aaacccctgt gaagtggacg gcaggaggag ctttcgctcc tctgaagggg cacatgaacg
    16981 tcccgtcgcc ggtatccggt ttatctcaca tctttcagtc aggaggttct tatgagtgca
    17041 cgtggtatca acaaggtcat cctcgtcggg cgtctgggca atgatccgga agtccgttac
    17101 atccccaacg ggggcgcagt ggcaaacctg caggtggcta cgtcagaaag ctggcgcgac
    17161 aaacagacgg gggagatgcg ggagcagacg gaatggcacc gcgtggtgct gttcggcaag
    17221 ctcgcggaag tggctggtga atatctgcgc aagggcgcgc aggtctacat cgaggggaaa
    17281 ctccgtaccc gtagctggga agataacggt atcacccgtt acgtcactga aattcttgtt
    17341 aagaccacgg gcaccatgca gatgctgggg agtgcaccac agcagaacgc tcaggcgcaa
    17401 ccgaagcctc agcagaatgg gcagccacag agtgctgacg cgacgaaaaa aggtggcgcg
    17461 aaaacgaaag gccgtggacg taaggccgcg cagcccgagc ctcagccgca aacgccggag
    17521 ggggaggatt acgggttttc agacgacatc ccgttctgat cgggctgact gtgacaaccg
    17581 ccccgccctg tgcggggcat caccggagat atgaggatga gcgaatattt cagaatactt
    17641 cagggactgc cggacggctc ctttacccgc gaacaggcgg aagccgttgc cgcacagtac
    17701 cggaacgtct ttatcgagga tgatcaggga acgcattttc gcctggttgt ccgcaataac
    17761 aatggcctgg tctggcgtac ctggaatttt gaggacggtg ccgggtactg gatgaaccat
    17821 gtcatccgga atttcggcat tcttaagtaa gagaggtgcc ggacgcgcaa acgtccggca
    17881 ggacataagc aattataagg ggatgattat gcctgtaacg aagtgtgaac cagaaaccac
    17941 ccgcaaagca agccgtaaat ctgtaaaaac gcaggaaact gtcctgtctg ccctgctggc
    18001 gcagacggag gaagtgagcg tgccgctggc ctcgctgatt aaatcaccgc tgaatgtgcg
    18061 cacggtgccg tattctgcgg agtccgtcag cgaactggcg gagtccatta agggcgtcgg
    18121 actgctgcag aatctggtcg tgcataccct gcctggtgac cgttacggtg tcgccgcagg
    18181 tggtcgccga ctggctgcac tcaacatgct ggcagagcgt ggcatcattc cggctgactg
    18241 gcctgtacgc gtgaaggtta ttccgcagga gctggcgact gccgcatcaa tgactgaaaa
    18301 cggtcatcgt cgggatatgc accctgccga acagattgcc ggattccgtg cgatggcgca
    18361 ggaaggcaaa acgcctgcac aaatcggtga tttgctgggc tattcgcccc gccacgttca
    18421 gcgaatgctg aaactggcag accttgcgcc tgtcatcctc gatgcgctgg cagaagaccg
    18481 catcaccaca gagcactgtc aggcgctggc gctggagaac gacaccgcgc gtcaggtgca
    18541 ggtgtttgaa gccgcctgcc agtcgggatg gggcggtaaa ccggaagtac agaccattcg
    18601 tcgtctggtg accgaaagtg aagtggcggt ggcggggaac agtaaattcc gcttcgtggg
    18661 ggctgatgcc ttctcgccag acgaactgcg caccgatttg ttcagcgacg acgagggtgg
    18721 ctatgtggac tgcgtggcgc tcgatgccgc cctgctggaa aaactccagg ctgtcgcaga
    18781 gttccttcgg gaagccgaag gctgggaatg gtgcgccgga cgcatggagc ctgtcggtga
    18841 gtgccgtgag gatgccggaa cataccgctg tctgccggag ccggaagcgg tgctgacgga
    18901 ggcagaagaa gaacgcctga acgaactgat ggcacgttac gacgcgctgg aaaaccagtg
    18961 tgaggaatcc gacctgctgg aagcagaaat gaagctgatt gactgcatgg cgaaggtcag
    19021 ggcgtggacg ccggagatgc gtgccggaag tggcgtggtg gtgtcctggc gttatggaaa
    19081 cgtatgtgtc cagcgtggtg tgcagttgcg cagtgaagat gacgcgactg atgacgctga
    19141 ccgcacggaa caggtgcagg agaaagcgtc agtggaggaa atcagtctgc cgttgctgac
    19201 gaaaatgtct tccgaacgca cgctggcagt ccaggcagca ctgatgcagc aaccggacaa
    19261 atctctggca ctgctggcat ggacgctctg cctgaatgtg tttgacagcg gagcgtacag
    19321 taaaccagca caaatcagcc tggaatgtaa acattattcg ctgaccagcg atgcgccgtc
    19381 agggaaggaa ggtgccgcat tcctggcgct gatggcagaa aaagcccgtc ttgcggccct
    19441 gttaccggag ggatggtcac gggacatgac gacgttcctg tcactcagtc aggaggtgct
    19501 gttatccctg ctcagtttct gcaccgcatg cagccttaac ggtgtccaga cccgcgagtg
    19561 tggtcgcacg tcacgcagtc cgcttgactc actggagagc gccatcggtt ttcacatgcg
    19621 cgactggtgg cagccgacga aagcaaactt cttcggacac ctgaaaaagc cgcagattat
    19681 cgcagccctg aatgatgccg gactgtccgg tgccgcacgg gacgcagaga agatgaagaa
    19741 aggcgatgcc gctgaacatg cagagttcca tatgaaagac aaccgctggg tgcctggctg
    19801 gatgtgttca ccacgtccac agacggatgc cactgaacgc gccgataacc tggctgatgc
    19861 cgcctgatga acaaccacac cgccccgccg gagacggggc ggcagaggga gaaatacgat
    19921 gatgaatacc gaactgacac tgaatgccct gcagtccatg aatgcccagg aatatgaaga
    19981 aatccgtgct gcgggaagcg atatgcgccg taatctcact cacgaggtga tgcgtgaagt
    20041 ggacgcaccg gctaactgga tgatgaacgg agagtatggc agcgagttcg ggggcttttt
    20101 ccccgtccag gttcgtttca cgccagccca cgaacgtttc cacctggcat tatgttcgcc
    20161 gggagacgtc tctcagctct ggatgctggt tctggtgaat ggtggtggac agcctttcgc
    20221 cgtcgttcag gtgcaacata tcttcacgcc tgtcgcgatc agtcacacgc tggcgcttgc
    20281 cgcgacactg gatgcgcagg gatacagtgt taacgacatc atccatatcc tgatggcaga
    20341 aggaggtcag gcatgagcgc acgttcacgg gcactgatcc ccctcagcgc agaacaacag
    20401 gccgccatgc aggcggtggc tgtcacagaa caacgtcgtc gtcagggacg cacactttca
    20461 gcatggcctt atgccaccgc tttctttcgc tgcctgaatg gcagtcgccg gatttcgctg
    20521 accgatctcc gcttttttgc ccctgcgctg acgaaggagg aatttcatgg caaccgtctc
    20581 ctgtggctgg ctgccgtgga taaactgatt gaaagttttg gggaagtctg tgttcttccc
    20641 ctgccatccg atgcggggca ccgtctgttc ccgtccgtgc cttttcgtga aggtgagcgt
    20701 cgtcgtcaga aaaccacgct gacagagcag aaatacagcc gccagcggga acgtgaggca
    20761 gaacgacggg aactggaata ccagacatgt tttgctcagg cgcagattga ccttgcgttt
    20821 catactcccg ccacggtcgg aagctggttg tcccgctggt ctggtgttgt tgaggagcat
    20881 gatctggaaa cgattttctg ggggtggtgc gggcgttttc catcactgtc atcatttgac
    20941 cggtttttct ggcaggagga accactctgg cggttgattt ttgaagccgg tgaggccggt
    21001 cgtggtgcac cggtacaggt acgtgcactt gagcagtgga tgatcccgaa caagctggag
    21061 aacgtaatat gatgaaatca gacgaaaaat accaggttcc cgcgtggatg cgacctctgt
    21121 tgccgttgct ctgcaacacc ggggggaacg atccggaaga actgctgaat gatacagaaa
    21181 ccactgccag tgcgaatgtt gtccgttatg tactgatagt tgctgtgcgg tcgcaggttg
    21241 atctgctgca gcttctgtac aggaaagggc tgttgcgcac agagatacca ggtggctttt
    21301 caccggaaga agcgcaggca ctcctggata atctggtgcg cagccatatc agcaaggcgc
    21361 tgtcgggcga gcgaatggca gcccgtgaca gaaatgccga tctggcctgg attggacagc
    21421 aactggtcga tgccgcctgg tttgtccgtg ccacactgga agcgcatggg atgagcgtcg
    21481 gaaatgagag tccccctgct ccgccggaga caatgccgga catacagaca cgggaactgg
    21541 tgatgttgat caagcgactg gcatcatcgc tgaaagcggt gaaaccggac agttgtgtgg
    21601 tgcgtgaagc gcaggactgg ctgcgcgaca gaaaacttgt ggatatcaca gatattctcc
    21661 ggtgaaccag gcatgtatat tttacgcagg gacatgccga tcatgatcgt ttcgttccag
    21721 gggtggtctg gagatcatgc ggtgtgctcc ctgctctcgc cggaataagg acgtcgccgc
    21781 cggctccgct tttacccggc catgcggggc atggccttgt gggttttcag ttatgtggcc
    21841 tcagcgtcgt gtgcgggctg tgccgtgcct ccatcgtagc cgggcttctg cgggcgggtt
    21901 gcaaggtaag atgaaccgct gcggtcaccc ggtccctcct tcccgtctgc cgtgattttc
    21961 cggtcgttca cccgctcaga tttcgcggtc tcaccctcca actcccgcca gccgtgtgcg
    22021 tcaggctgcg gcttcccttg cgcccttgca accctgcagg cccggcaaag atggaggcac
    22081 ggcaccgccc ggtgtcgcag aacatgtgac tatggaggat tcgggaatgt ctgttgttgc
    22141 acctgctgta tacgttggaa cctggcacaa atacaactgt ggaagcatcg ctggccgctg
    22201 gtttgacctg acaacgtttg atgatgaacg cgactttttc gccgcctgcc gtgcccttca
    22261 ccaggatgaa accgacccgg aactgatgtt tcaggattat gagggattcc cggggaatat
    22321 ggcctctgaa tgccatatca actgggcctg ggttgaaggc ttccgccagg cgcgggatga
    22381 aggctgcgaa gaggcttatc gtctctgggt ggatgatacc ggtgagacgg attttgacag
    22441 cttccgcgat gcctggtggg gcgaggctga cagtgaggag gcttttgcgg ttgagttcgt
    22501 cagtgatacc ggcctgctgg ctgacgtgcc ggagacggtg gcgctctatt ttgactatga
    22561 ggcgtatgcg cgggatttat tcctggactc cttcaccttt attgacggtc atgtgttccg
    22621 tcggtgattt actccccgct ccggcggggg tatggcctgc caggcccccg gaaaaccctg
    22681 gcaggcttac gcctgcctga aatacaggag ggccgcgcct gcggcgctgc ccttcctgcc
    22741 tgtcatagtg tgaggcattt ttctgttttg tcgtttctgt tgttctgact cccgggggcg
    22801 gggacaccct tcggctcccg gctgcctggc ggcatccggt cgtcaggccc gactccgaca
    22861 ggcagctgct ggcgcacccg ccagtctccg ccgaacccgc tggcgcggtt tcgggctgcc
    22921 gctacggtcc gatgtctgaa aaaccaggct tgcagcgacc gttccggtcg cgccgcctct
    22981 gtcgcgccgt gacctgcgct ccctgcacac ctgacggtgt gtaaggaaac tgcgcctgat
    23041 ggcgcagttg tctgcccttc cgccgccacc cctgcggcct gtttcccgct gtggactttc
    23101 cgggggctga agcggccaca acacgtgtca gccgtccagg ggggagatgt accagccaca
    23161 aaactttttg ccgcaagcgt caaaaacttt tgcgtctgcc cccggacaac tgccccgtgc
    23221 cgtggcagtc gctgccccat cggaaaggtc cactgcgggg agaaaccccg aagaagtgga
    23281 cggcagaagg ggctttcgcc cctccggggg agcaaccggt cggcccggcg acctgattca
    23341 gcagaaggag acgttttcat gacagtcagc agcacgattt ccgttttttg ccgcgacggg
    23401 gtgttccgta ccgtttactg ccacctgcac ggtgagccga cctggaacgg tcgcatcctg
    23461 catacccact atgccaccgg tcagcaggcc gaagccctgg ttgaacacgg tgatatccgt
    23521 tgcctcggtc cccgttgcga caaacccgcc gggcatacgc ttcagaaccc ggtggacggt
    23581 gtgacggctt attacggacg tgacagtggt ttccggatgg acagtgaggc gcgtgagtac
    23641 cgttctttca gggaggctat tgccactgaa agcactgaag aggtgcgctt ccattatgtg
    23701 ttcatcgacg gctactggaa ggtgatgtac cgcacgccgg aaggctggaa gatgaaagcg
    23761 ctcgcgctgg cactgcgtcg ctgtccggaa tgaaaaaagc agggcgataa accctgcctt
    23821 tctccgctgg cgcttccccg tacggaagtc cggcatctca ttcactaact atggagatta
    23881 tgccatgaga ccatcaatta tcttcgcaac cgccgagtat gtaaagcgtc tgcgtgaaga
    23941 gtgcctgcgg gagaacaaac ccctgcaccg ccatacccgc ttcagacgcc aggaactggc
    24001 accggatgag attaacccgg acgtcctggc gatgggcggt catatcgcca gacgctgcag
    24061 tgagcagaag cgggtacgta tccccgccat gaaagtcagc gaatggggcc acctgctccg
    24121 cgcgcttgaa attgaacggg tctgtcactg attaacccct gccgtctgcc tgtgcaggtg
    24181 gcaggatgtg ttcaggtact ttaccaggag gaaccgtgag caagatgaaa aacatcacga
    24241 cgtctgtgtt gcatggtgtc tccacaaaaa acagaaaagg gaaacagatt ctctttcatt
    24301 cagggcccgg gagggctgga gataacaatg aagtcttgtt ttgatactga caaagctttt
    24361 catatgtctt ttgatgaagt cactttcaac gctgacagaa ttcgctcaaa actggaacta
    24421 ttatccgaag aactaatgcc ccgttgtggt cttgtttatg agctatatca gcgacgtttg
    24481 agtgctgcta ttgataattt tgtggccaca ttgccgacag aacaacatct actggtcctg
    24541 gaactggccc gtgaggagtt tgattacctt tccgcagaag agattgctga agaaatacgc
    24601 cgtgactcag aacgcggcta ttgttgtcat ggctttgaac gcaattgctg tcctctcggt
    24661 tgtggggatt tgggcgatga ttgctgcgat gctgctgatt tgatggagga accgtgagta
    24721 agaagaaaaa caccacaacg cccacgccgc atgatgccgc gttccggtcg ttcctggcga
    24781 atcccgacgt cgccagagat tttctggaac tgcatcttcc ggcggagtac cggcagttgt
    24841 gcgacctgtc cacgctgaag ctggaacccg ccacctttgt agagccggac ctgcatcagt
    24901 acgccagcga tatcctctgg agcgtgaaaa ccaccggggg tgaagacggc tatgtttata
    24961 cgctcatcga gcaccagagc accgaaaatc tgtacatgcc ttttcgcatg ttacgttaca
    25021 gtgtggcggc gatgcagaga catctggagc agcacaaaac attgccactg gtcattccgg
    25081 tgctgttcta tcacggtgag cgcagcccgt acccgtacag catgaactgg ctgggctgtt
    25141 ttgagaatcc ggctcttgcg gctaaaatat acacaaagcc gtttccgctg gttgatatca
    25201 ctgtcgttga tgacaatgaa atcatgaacc atcgccggat ggccgcactg acgctgctga
    25261 tgaagcatat ccgccatcgt gacatgatgg agctgctgga caaactcccg caggtcatgg
    25321 tggaaatttc agacgagcag gtgcgtgttc tgattcatta catcgttaac gcaggggact
    25381 ctgtatcacc ggaatttatg cgggcgctgg ctgagcgtct gccgcagcat gaggataaac
    25441 tgatgactat cgcagaacgt cttgagcaaa aaggtcgcca ggaaggagcg cttgaaaaag
    25501 ccctggctat tgcgtgccag cttcagaaaa tggggatgac gccggagcag attaagcagg
    25561 ctaccggact ttccgatgac gaactgaaga aaatcactca ctgatgagaa ccacaaagcc
    25621 cggcaaaatg ccgggctttt ttgtcagcag gagcagagca aacagcatga cgattgcaga
    25681 acgtcttata cagaaaggtt tcgacgaggg ttttaaacaa ggggctctag aagtggcgcg
    25741 ggaaatagcc tgccggcttc gggatatagg ctggccgccg gaacggattc aggaggtcac
    25801 gggactttcc ggtgaagaac tgaaaaagct gtttcctgat gagcagtagc ctggcattca
    25861 gccaggctgt gaatcacgct cacacattcc ctgccgtttc ccccagttca ttttccagtc
    25921 gcctgatgat atctgcctta cgcatcccct gcatgtgagg cagcaggtca cggactgaaa
    25981 gtgcctgggt ggtcctccag acggtgacgt ccacctgttc cgcattttcc caggcgtgcc
    26041 atgttctctg cgtcacgcca aattctctgg ccgctttttc gcgtgaccag agcatgcttt
    26101 ttcgccagat tcgcagttcc cagccggtca taaaacacct ttgaaaaagt gaaaaaattt
    26161 cactttattt tatcatcata atcaccaaat caacgcgaaa aataagcgtg aaaaagtgaa
    26221 gaaatttcac ttttttttgg ttgtaaatgt cgtgcggttt tctctgtttt cacctgtgcg
    26281 ggagtggtgt gccagtgacc accacatgcc attgttgtgc cgtcgttttt cttcagtcgc
    26341 gccgtatgcg gcgctccctg cacaccttac ggtgtgtaag gccactgcgc ctgatggcgc
    26401 agctgtctct gcctgtctgc cgccgcactg cggcctgttc tccgcagacg gactttccgg
    26461 gggtacatac atgaccacat cccgtttcag tcgtccaggg gagagatgta ccagccacaa
    26521 aactttttgc cgcaggcgtc aaaaactttt gcgtctgccc ccggacaact gccccgtgac
    26581 gtggtgagcg tcccccgtcg gaaaggtcca ctgcggggaa atccccgaag acccggacgg
    26641 cagaaggagc ttcgctcctc cgggaaatga cctggctgac ccggcagtcg tgacgatgag
    26701 gagacaatgt gatgaatcag actttaccca ctgctgacct gaatactgcc ggcacgacag
    26761 atgttattcc gtctgtagct atcgaccgca tcatcgcgca gcgtaacgaa ggtattgcac
    26821 tgttcatgca ggcgatggaa tgcctggcga cagcgcgcaa gattctgctc gatgcgtcag
    26881 gtgatatttt tctttacggg tttgaagact gcgtgactga ttccgttcgt cgcatggata
    26941 agacggaaga agcgaaaagg aatatcaccc gtcttgccga ccggaaaatc tgggaccgcc
    27001 tgatgacaga tacgggcatg tacaccttca tgagttcatg ccagcgtgat gagtggaaca
    27061 gccagctgat gagcgacacc tgtcctgaaa tcaccctgga caatgtactg gcaacattcc
    27121 gccatcttaa tgccagcaag atgcagacat ttgaacaggg actgattgat gtctaccgga
    27181 aattgtcatg ggattacaga accaataatc cctgtcgtct tggcaagaga atcattattg
    27241 aaaacctgct gtaccgctgg agtaacgggc gtatgacgct ggactgcagc ggacgggagg
    27301 cactggatga cctggtacgt cctttttatc tgctggagga gcgcaacgtt cctgacttcc
    27361 ggagcagcat cggggcgcag tatggtgaat ttctcgggaa cggcgacaat gtcggtaagc
    27421 tgttagaagg agaatatttt acggtgcgtg gctaccagaa agggaccgta cacattgtct
    27481 ttaagcgttc tgaccttgtt gaaaaactga atgatattat tgcacggcat tatcctggtg
    27541 cattgccgcc acgagtctga ttaaacagaa aagcccaggt atttatgccg gggctttttt
    27601 ttctgtaatc atcataatct cagaacacaa tgtgacattg tcacatcaat tgttgttcat
    27661 atcgatgcca tctttcctgc ctgaatctgt ctttttaggt gctgttttag aggaagaacg
    27721 gcgggatggt tttttcgatt ctgcttcccg aataatgtca cgaagtgctt tctctcctac
    27781 ttcataccct gcatttttga gcgtgtcacg aacatctgtc agtgtataac cctttgtcct
    27841 taccaggata atgatgtcat cacgaatggc atcaagaaaa tcacgcaatg ttttccgttg
    27901 agcggtgagg tctggcaatt ccgataaagc agcttttgcc agctggatgt catcatctga
    27961 gtaaaatttt ttagacgcca ttggcacttt ctctccgttt aatgttgaag cctgatttta
    28021 tcatcaaaaa ctgactcctg gcagggctgc gccctgcctc tgccgtctgc ataagactat
    28081 gatgcacaaa aataacaggc tataatggac tgaaaaacgg gacaggatgt gggaatgtcg
    28141 gaccgcgcac gcgcactcag acattcccca cctggtcagg gctttgcccc gacaccccgg
    28201 taaggagcag accatgagtg agaagaaaac ccgaacgggc agtgagaaca ggaagagaat
    28261 tgtaaaattc acggcgagat tcacggagga tgaggctgaa atcgtaagag aaaaagcgga
    28321 gtcaagtgga cagacggttt caacatttat acgaagttct tcactggata aggtggttaa
    28381 ctgccgtact gacgaccgta tgattgatga gattatgcgt cttggccgcc ttcagaagca
    28441 tctgtttgtg gagggaaaac gcactggtga taaggagtat gcagaggttc ttgttgccat
    28501 tactgaatat gtcaatgctc tgcgaagaga cttaatggga cgctgagata actgctgatg
    28561 aatgccatta ttccgcacaa aagacgtgac ggcaggtctt cctttgaaga cctgattgcg
    28621 tacacgtctg tgcgggatga tgtgcaggaa aatgagctca cagaagacag cgaaatcaag
    28681 agtgatgtcc ctcaccgcaa ccgtttcaga cggcttgttg attatgcaac acgcctgcgt
    28741 aacgaaaaat ttgtctccct gattgatgtg atgaaggacg gcagtcagtg ggtaaatttc
    28801 tatggcgtaa cctgtttcca taactgcaat tcactggaaa cagctgctga agagatgcag
    28861 tacaaggcgg acaaagctgt tttttcccgt aatgatacag accccgtttt ccactacatt
    28921 ctttcctggc ctgcgcatga aagcccgcgt cctgagcagc tttttgactg tgtgcgtcac
    28981 acactgaaat ctcttgaact ctcaaaacat cagtacgtgg ctgccgttca tacggacacg
    29041 gataatctgc atgtgcatgt tgccgtgaat cgtgtacatc ctgaaactgg ttatattaac
    29101 tggctttcct ggagccagga aaaactcagc cgtgcatgtc gtgaacttga acttaagcac
    29161 ggattcgcgc ctgataatgg ctgctttgtg catgcacccg gcaaccgtat tgtgcgcaaa
    29221 acagcgctgg tacgtgagcg ccgtaatgcc tggcgacgcg ggaaaaagca gacgttcagg
    29281 gaatatattg cgcagatgtc gattgccggg ctgcgtgaag agcccgcaca agactggttg
    29341 tctttgcata aacggctggc cagtgatggt ctgtatatca cgatgcagga aggggaactg
    29401 gtggtgaagg atggctggga cagagcgcgg gaaggtgttg cactcagttc gttcggaccg
    29461 tcatggacag ctgaaaaact ggggcggaaa ctgggggaat atcagcctgt accgacagat
    29521 attttcagtc aggtggggac acccggtcgt tatgatccgg aagccataaa cgttgatatc
    29581 cggccggaaa aagtggcaga aacggagagt ctgaaacagt acgcctgccg tcattttgcc
    29641 gaacgcttac cggaaatggc gagaaatggt gagctggaaa gttgcctgga tgtgcacaga
    29701 actctggcaa aggctggatt atggatgggg atccagcacg ggcatctggt actgcatgat
    29761 ggatttgata aacaacagac accagtacgt gcagacagtg tgtggccgtt aatgacgctg
    29821 gattatatgc aggatctgga tggtggatgg cagcctgtac cgaaggatat ttttactcag
    29881 gttatacccg gagaacgatt ccggggacgt aatcttggta ctcaggctgt cagtgattac
    29941 gaatggtatc gtattcggat ggggacaggt ccacagggag caataaagcg cgaactgttt
    30001 tccgataagg aaagtctgtg ggggtacacg actgttcagt gtgagtcgtt gattgaagat
    30061 atgatcgcgg gcgggaattt cagctggcag gcgtgccatg agatgtttgc gcgtaaaggg
    30121 cttatgttgc agaaacagca tcatggtctt gtgattgtgg atgcgtttaa tcatgagctc
    30181 acaccagtga aggccagcag tatccacccg gatttgacgt tatccagggc tgagccgcag
    30241 gcagggccat ttgagattgc aggtgcagat atatttgaac gggtgaagcc tgaatgtcgc
    30301 tacaatccgg aacttgctgc cagcgatgaa gtggagctcg gcttccggcg cgatcctgta
    30361 ctgcgtcgtg aacgccgtga agcccgtgca gctgcgcggg aagatttgcg cgcgcgttat
    30421 cttgcatgga aggagcactg gcgtaaaccc gacttacgtt acggagaacg gttacgggaa
    30481 attcatgctg cctgccgtcg gcgcaaggcg tatatccgtg tgcagttccg ggatccacaa
    30541 ttgcgtaagc tgcattacca tattgcagaa gtgcagcgta tgcaggcgct gatcaggctg
    30601 aaggagagtg tgaaggaaga gcggttgtca ctgattgcgg agggtaagtg gtatcctttg
    30661 tcctaccggc agtgggtgga gcaacaggcg gtacagggag acagagctgc attatcacag
    30721 ttacgtggct gggattatcg tgatcgtcgc aaagacaagc gacgaacaac gaatgccgac
    30781 cgctgcgtga ttctctgtga accgggagga actccacttt atgaagacac tggcgttctc
    30841 gaagcccgtc tgcagaaaga tggcagtgtg cgttttcgtg atcggaggaa tggcgagtta
    30901 gtctgtgtgg attatgggga ccgtgtggtg ttctaccatc atcaggacag aaatgaactg
    30961 gtggataagc tgaatctgat tgctcctgta ttgtttgatc gtgagccaag aatgggtttt
    31021 gaaccggaag gctcatatca acagtttaat gatgtatttg cggaaatggt ggcctggcat
    31081 aatgcagctg gaataaccgg aagtggacac tttgtaatca gccgcccgga tgtggattta
    31141 catcgtcagc gtagtgaaca gtactaccac gaatatatca ggcaacagaa aagcatatcc
    31201 ggggggcatg gcgcatctta tgctccagtt caggataatg agtggacgcc accatcacct
    31261 ggaatgtagg gatgaggagt ggatattaaa aatcttaact tgtattagtt aagattttct
    31321 ttgctctgac agagctatgg tactgagatc tatatggatt gaggagcaat atatgggggt
    31381 aaagaaaatc gttatgctgg ctattttagt cagctttgtg gcgggatgtg cgccattaca
    31441 tccttcaaat tgccataaaa ctacagccct gggtagttgc agttcaggac gttgggatga
    31501 tcaggatgaa tggggggagc aagcacgggg aatcagggat gcaattaatg ctaaacttga
    31561 tgatccgcaa aaatggaaaa ggaaaaaatg caggttgcat atggaattct cccgggatgg
    31621 cacggcgtta aaaatatcaa ccagtaatgg cgataaagcc tattgcgaag cgataaagtc
    31681 agcagcccat aaagctaaat tcccggcctt caacaatccg gaagtttata gagattttca
    31741 aaaatccggc tttgacatgc gcggttagct ccacgacata tctgtagggc taacagaaga
    31801 ctgtcaggtt ggctgattac tacactggaa cctgaggtac tttgcctcag gttatgttga
    31861 catactcaaa aatagattct tacattatcc cggtaacggc gtccttcatc ggcctgccag
    31921 ttaccatcaa tggtattggt accctcgttc tttctgtctg tgatgttaat aagggcaaaa
    31981 ttaatcagca tgtctggcag gagtgacggg cactggctgg caatgtctag caacggcagg
    32041 catttcggct gagggtaaaa gaactttccg ctaagcgata gactgtatgt aaacacagta
    32101 ttgcaaggac gcggaacatg cctcatgtgg cggccaggac ggccagccgg gatcgggata
    32161 ctggtcgtta ccagagccac cgacccgagc aaacccttct ctatcagatc gttgacgagt
    32221 attacccggc attcgctgcg cttatggcag agcagggaaa ggaattgccg ggctatgtgc
    32281 aacgggaatt tgaagaattt ctccaatgcg ggcggctgga gcatggcttt ctacgggttc
    32341 gctgcgagtc ttgccacgcc gagcacctgg tcgctttcag ctgtaagcgt cgcggtttct
    32401 gcccgagctg tggggcgcgg cggatggccg aaagtgccgc cttgctggtt gatgaagtac
    32461 tgcctgaaca acccatgcgt cagtgggtgt tgagcttccc gtttcagctg cgtttcctgt
    32521 ttggggtcgt ttgcgggaag gggcggaatc ctacgctaag gctttggcca gcgatattct
    32581 ccggtgagat tgatgtgttc ccaggggata ggagaagtcg cttgatatct agtatgacgt
    32641 ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg cgttgctcct cttctccatc
    32701 cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc tggtcgagca ggcgacgcgg
    32761 atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt gcaatgcgtc taggatcgag
    32821 acaaaggtcg tctctgtcag ccggatcgta gaacatattg gcggcgccaa agcccacttc
    32881 accgaccaga ccgacgggat ctatcaccag ccagccgcga ctggagaaca tgatgttttc
    32941 atgatgcaga tcgccatgta gcccacgcag ttccgaggca ttgctcatca tt
//
LOCUS       DAZGJT010000046        32663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZGJT010000046 DAZGJT010000000
VERSION     DAZGJT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.046"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..185)
                     /locus_tag="ACHKFK_002399"
     CDS             complement(<1..185)
                     /locus_tag="ACHKFK_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulA-like leucine-rich domain-containing
                     protein"
                     /protein_id="HFO9052722.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSR"
     gene            404..1033
                     /gene="sxy"
                     /locus_tag="ACHKFK_002400"
     CDS             404..1033
                     /gene="sxy"
                     /locus_tag="ACHKFK_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO9052723.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(996..3158)
                     /gene="yccS"
                     /locus_tag="ACHKFK_002401"
     CDS             complement(996..3158)
                     /gene="yccS"
                     /locus_tag="ACHKFK_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO9052724.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVLA"
     gene            complement(3168..3614)
                     /gene="yccF"
                     /locus_tag="ACHKFK_002402"
     CDS             complement(3168..3614)
                     /gene="yccF"
                     /locus_tag="ACHKFK_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO9052725.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFQ"
     gene            3737..5791
                     /gene="helD"
                     /locus_tag="ACHKFK_002403"
     CDS             3737..5791
                     /gene="helD"
                     /locus_tag="ACHKFK_002403"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO9052726.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGMRQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIEGARLQHNQAYTEAMLIEYADFFRQVESSPLNPAQARAVVNCEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLAFRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDEGQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVDILKNLDVPVAR
                     KP"
     gene            complement(5823..6281)
                     /gene="mgsA"
                     /locus_tag="ACHKFK_002404"
     CDS             complement(5823..6281)
                     /gene="mgsA"
                     /locus_tag="ACHKFK_002404"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO9052727.1"
                     /translation="MELTTRTLPSRKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6377..7034)
                     /gene="yccT"
                     /locus_tag="ACHKFK_002405"
                     /pseudo
     CDS             complement(6377..7034)
                     /gene="yccT"
                     /locus_tag="ACHKFK_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
     gene            7207..7620
                     /gene="yccU"
                     /locus_tag="ACHKFK_002406"
     CDS             7207..7620
                     /gene="yccU"
                     /locus_tag="ACHKFK_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO9052728.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(7665..7982)
                     /gene="hspQ"
                     /locus_tag="ACHKFK_002407"
     CDS             complement(7665..7982)
                     /gene="hspQ"
                     /locus_tag="ACHKFK_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO9052729.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8040..9230)
                     /gene="rlmI"
                     /locus_tag="ACHKFK_002408"
     CDS             complement(8040..9230)
                     /gene="rlmI"
                     /locus_tag="ACHKFK_002408"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO9052730.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFTRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPACAIYDRSDVA
                     VRKKEGMELTQGPITGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9325..9603
                     /gene="yccX"
                     /locus_tag="ACHKFK_002409"
     CDS             9325..9603
                     /gene="yccX"
                     /locus_tag="ACHKFK_002409"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO9052731.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGDEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(9600..9929)
                     /gene="tusE"
                     /locus_tag="ACHKFK_002410"
     CDS             complement(9600..9929)
                     /gene="tusE"
                     /locus_tag="ACHKFK_002410"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO9052732.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGIALSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10021..10680)
                     /gene="yccA"
                     /locus_tag="ACHKFK_002411"
     CDS             complement(10021..10680)
                     /gene="yccA"
                     /locus_tag="ACHKFK_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO9052733.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(10889..10976)
                     /locus_tag="ACHKFK_002412"
     tRNA            complement(10889..10976)
                     /locus_tag="ACHKFK_002412"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10940..10942),aa:Ser,seq:tga)
     gene            11403..12521
                     /gene="hyaA"
                     /locus_tag="ACHKFK_002413"
     CDS             11403..12521
                     /gene="hyaA"
                     /locus_tag="ACHKFK_002413"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO9052734.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            12518..14311
                     /gene="hyaB"
                     /locus_tag="ACHKFK_002414"
     CDS             12518..14311
                     /gene="hyaB"
                     /locus_tag="ACHKFK_002414"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO9052735.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTVDFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALKLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            14330..15037
                     /gene="hyaC"
                     /locus_tag="ACHKFK_002415"
     CDS             14330..15037
                     /gene="hyaC"
                     /locus_tag="ACHKFK_002415"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO9052736.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            15034..15621
                     /gene="hyaD"
                     /locus_tag="ACHKFK_002416"
     CDS             15034..15621
                     /gene="hyaD"
                     /locus_tag="ACHKFK_002416"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO9052737.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEDVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAYIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMRLEEQG"
     gene            15618..16016
                     /gene="hyaE"
                     /locus_tag="ACHKFK_002417"
     CDS             15618..16016
                     /gene="hyaE"
                     /locus_tag="ACHKFK_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO9052738.1"
                     /translation="MSNDTPFNALWQRMLARGWTPVSECRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            16013..16870
                     /gene="hyaF"
                     /locus_tag="ACHKFK_002418"
     CDS             16013..16870
                     /gene="hyaF"
                     /locus_tag="ACHKFK_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO9052739.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
                     IDGLMNGLPLAYELLAHVRNPDAQPHSINMTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEGAPT"
     gene            17004..18548
                     /gene="appC"
                     /locus_tag="ACHKFK_002419"
     CDS             17004..18548
                     /gene="appC"
                     /locus_tag="ACHKFK_002419"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO9052740.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQHPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLVIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQTAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            18560..19696
                     /gene="appB"
                     /locus_tag="ACHKFK_002420"
     CDS             18560..19696
                     /gene="appB"
                     /locus_tag="ACHKFK_002420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9052741.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            19709..19801
                     /gene="cbdX"
                     /locus_tag="ACHKFK_002421"
     CDS             19709..19801
                     /gene="cbdX"
                     /locus_tag="ACHKFK_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO9052742.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            19881..21191
                     /gene="appA"
                     /locus_tag="ACHKFK_002422"
     CDS             19881..21191
                     /gene="appA"
                     /locus_tag="ACHKFK_002422"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001623747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO9052743.1"
                     /translation="MKAILIPFLSLLIPLTPKSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAVIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGAIADFAGHRQTAFRELERVLNFPQSNLCLNRKKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPSQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACALHQDK"
     gene            complement(21179..21391)
                     /gene="cspH"
                     /locus_tag="ACHKFK_002423"
     CDS             complement(21179..21391)
                     /gene="cspH"
                     /locus_tag="ACHKFK_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO9052744.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            21677..21889
                     /gene="cspG"
                     /locus_tag="ACHKFK_002424"
     CDS             21677..21889
                     /gene="cspG"
                     /locus_tag="ACHKFK_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO9052745.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            21900..22088
                     /gene="ymcF"
                     /locus_tag="ACHKFK_002425"
     CDS             21900..22088
                     /gene="ymcF"
                     /locus_tag="ACHKFK_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO9052746.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            22063..22293
                     /gene="ymcE"
                     /locus_tag="ACHKFK_002426"
     CDS             22063..22293
                     /gene="ymcE"
                     /locus_tag="ACHKFK_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO9052747.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLIRVPGTNTLLACCCLLRPEEVNSEY"
     gene            22283..22456
                     /gene="gnsA"
                     /locus_tag="ACHKFK_002427"
     CDS             22283..22456
                     /gene="gnsA"
                     /locus_tag="ACHKFK_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO9052748.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(22505..23578)
                     /gene="yccM"
                     /locus_tag="ACHKFK_002428"
     CDS             complement(22505..23578)
                     /gene="yccM"
                     /locus_tag="ACHKFK_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9052749.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKTTQLLLLAINI
                     YIAITFWYWVRYYETAGSTTFITRPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQFVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISMIVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKSCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVMGIMFAAIGYAMYAG
                     VWQSPVPEELYQRLIPQSPMIGH"
     gene            complement(23650..26393)
                     /gene="torS"
                     /locus_tag="ACHKFK_002429"
                     /pseudo
     CDS             complement(23650..26393)
                     /gene="torS"
                     /locus_tag="ACHKFK_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            26476..27504
                     /gene="torT"
                     /locus_tag="ACHKFK_002430"
     CDS             26476..27504
                     /gene="torT"
                     /locus_tag="ACHKFK_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO9052750.1"
                     /translation="MRVLLLLLLSLFMLSAFSADNLLRWHDAQHFSVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPHVKSRVGVPWFQMGYQPGRYLVQ
                     WSHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVMTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(27477..28169)
                     /gene="torR"
                     /locus_tag="ACHKFK_002431"
     CDS             complement(27477..28169)
                     /gene="torR"
                     /locus_tag="ACHKFK_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO9052751.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            28299..29471
                     /gene="torC"
                     /locus_tag="ACHKFK_002432"
     CDS             28299..29471
                     /gene="torC"
                     /locus_tag="ACHKFK_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO9052752.1"
                     /translation="MRKLWNALRRPSARWSVLALVATGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            29471..32017
                     /gene="torA"
                     /locus_tag="ACHKFK_002433"
     CDS             29471..32017
                     /gene="torA"
                     /locus_tag="ACHKFK_002433"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO9052753.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFISPQDASARGIRHGDV
                     VRVFNVRGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            32014..32613
                     /gene="torD"
                     /locus_tag="ACHKFK_002434"
     CDS             32014..32613
                     /gene="torD"
                     /locus_tag="ACHKFK_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO9052754.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNGLENSIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDGLRQKTLTA
                     LREWLPEFAARCRQYDRFGFYAALSQLLLVLVECDYQKR"
BASE COUNT         7926 a         8210 c         8795 g         7732 t
ORIGIN      
        1 cgcgattgct gaccgagttg ctgtaacaat ggcaacagta gaagttgcgt catcatgggc
       61 tgatcttcgc gatagacaac ttcactgata agcccggctg tagtgttttc cgtagagaca
      121 cgcgcaattt tacttgctgc ggatgagaac gacgaagaac gatgtgcata gcctgaagtg
      181 tacataatca atccagcccc tgtgggttgc tgtatgaata tacagtacat ccagtgacga
      241 caaagatcaa ccctattttc ggaaagagcc tcgcaaattt tgtcgttggt ggcgggaaaa
      301 cataaattaa tcttgcccct taagaataag ttgcctattt tcgtagttaa cggatccgtt
      361 aatgtgaatc attcttttat gttatgattt taaaaggaat tttatgaaaa gcctctccta
      421 taagcggatc tataaatcac aagaatacct ggcaacgttg ggcacaattg aataccgatc
      481 attgtttggt agttacagcc tgaccgttga cgacacggtg tttgcgatgg tttctgatgg
      541 tgagttgtat cttcgggctt gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt
      601 ctggctgaca tataaaaagt gtggccgatc cgttaccctc aattactatc gagttgatga
      661 aagtctatgg cgaaatcaac tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc
      721 gctgaaagag aaaagcacgc gcaatacccg ggaaagactg aaagatttgc ccaatatgtc
      781 ttttcatctg gaagcgattc tcggggaggt ggggattaag gatgtacggg cgttacgtat
      841 acttggggca aaaatgtgtt ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa
      901 gattctgttt atgcttgaag gggccattat cggtattcat gaagctgcgc tcccggtggc
      961 acgccgccag gagcttgcag aatgggctga ctctcttacg ccaaaacagg agtttcctgc
     1021 ggaacttgag taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac
     1081 ccaacttgtt gtacgaccag aggttctttg ctgtctgccc gtggttcaag ttgctgcatc
     1141 cgctgtttca ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt
     1201 agcgcgtcat caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt
     1261 aactgctccc ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac
     1321 agcaaccgaa acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt
     1381 gataccaccg acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac
     1441 gccagacggt tatcacgccc ctgatggtat tgttccagta tggcatcgag ataccggcag
     1501 ttggcctctg tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag
     1561 atgtagctca ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt
     1621 aacgccactt caaaaccttc acccagcaag ttgaaacaca gtagcaccaa aagtgtgatg
     1681 aacatcgttg catgggcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata
     1741 accagcagca ccagctgccc ttccagtgat ggcacaaacc acagcacagg aataccaatt
     1801 gcgataccta ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag
     1861 tttggctggc agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt
     1921 atctgaatga tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag
     1981 agggcggatt ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc
     2041 gggctgtcat cagcgagctc attttcgtca ttattatggg gcagtgcctg ggcctgttct
     2101 gattcaattg ttgccagttg ggcatcaatg gcacgtaaat tgttcagtaa aaatcccagt
     2161 gttttgagta aatcagcagg agcgccgtta tcgcgcatcc gctccagcgc agcatcaata
     2221 tgcgtaaaag cgcgctcaaa atgcggatca tgttgataag gctgacgcag caaaatacag
     2281 cgtgacagtt gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc
     2341 acgtcgctgt ggcgaaaatg ttcacgcaat gtttgatatt gaatatgaga agagctggca
     2401 cgctcgtgaa tatcctgtgc gacaaagtaa taatgcagtg tgcgacgcgt tccccgttga
     2461 ccacgatcgc cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc
     2521 agctgaccgt tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca
     2581 ggatcaaaca tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc
     2641 aggttgtcct gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg
     2701 taccagacgg caccggccag aagatacatc ggctgctgat accagtgctc atacagtgat
     2761 gttcccaaca tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt
     2821 tgacccagac cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat
     2881 agccagggcc agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc
     2941 gtaatgatga ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct
     3001 gccaccatcc caagcgtcag cggaatcgtc agttttacat cacccagcca ccacggaaac
     3061 gctgtggttc cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccaa
     3121 gtatagcgtt tgagcaaagg acttagcata aaggccatat tagtggatta ttggaaacga
     3181 cgacgtgcat tggcttcgcg cgcagcttgc gctgtttcta ccgataccac gcgacgaccg
     3241 accggccata gtgcaatagc ggcaatttta aagttcgcaa taccgacagg aatgccaatg
     3301 atggaaatac attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag
     3361 aaaatcagcc agaaaatatt caataccgta ccgccagtat ttagcagcac atttttgcca
     3421 gccgggttca gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta
     3481 gtgatctccc agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga
     3541 gtcgccaaca gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc
     3601 aaaacggtac gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa
     3661 cgtttttttg ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt
     3721 gtggatctac agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca
     3781 cccttacgat cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga
     3841 ataccttatt cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg
     3901 cgaactggag tttgtactgc cggacgaaaa agtggtgcgt ctgcacggta ccgaatgggg
     3961 cgagacgcag cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat
     4021 gagcgaaatt gcgtctggcg ttttacgtca gcaactggat ttgattgcca cgcgcaccgg
     4081 agaaaataaa tggctgacgc gtgagcaaac ctctggtatg cggcaacaaa tccgccaggc
     4141 tttgtcggcg ttaccattgc cggttaaccg actggaagaa ttcgataact gccgtgaggc
     4201 gtggcgtaaa tgtcaggcct ggttgaaaga cattgaaggc gctcggttgc agcataatca
     4261 ggcgtatacc gaagccatgc ttatcgagta tgcggatttt ttccgccagg tcgagtcttc
     4321 accgctgaat ccggcgcagg cccgggcagt cgttaattgc gagcattctt tgttagtgct
     4381 ggctggtgca ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc
     4441 gcgtggtgaa gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga
     4501 agagatggac gagcgtattc gagaacgact acataccgaa gacattaccg cacgcacgtt
     4561 tcatgcgctg gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa
     4621 actggaaaat gataccgctg cccgtcatga actttttatt gctgagtggc gcaagcaatg
     4681 cagcgaaaag aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg
     4741 gtcagtgcca gaaggtaatt tctgggatga tgaaaaatta cagcgtcgtc ttgcctttcg
     4801 cctcgatcgc tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc
     4861 cagtgcaccc gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt
     4921 attaaaagcc tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat
     4981 tcaccaggcg attgtgattc tggagaaagg tcgctttatc agcccgtgga agcatattct
     5041 ggttgatgaa tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa
     5101 gcaaaacagt cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttatcgctt
     5161 cagcggtgcg caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggtgatcg
     5221 ctgcgattta gacacgacct accgttttaa cagtcgtatc ggtgaggtgg caaacaggtt
     5281 tattcagcag aacccaggcc agcttaaaaa gccgctaaac agcttaacca atggagacaa
     5341 aaaagccgtc acgttattgg atgagggtca acttgacgct ttgctggata aactctctgg
     5401 ttatgccaaa ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc
     5461 cagcctggaa aaagcagcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca
     5521 tgccagcaaa gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtga
     5581 tggttttccg gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga
     5641 ggatttcccg gacgctgaag aacggcggtt aatgtacgtg gcgctgaccc gggcacgcca
     5701 tcgggtatgg gcactgttta acaaagagaa tccctctccc tttgtggata tactgaaaaa
     5761 tctggatgtg ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa
     5821 tattacttca gacggtctgc gagataacgc tgataatcgg ggatcagaat atcgaccgcg
     5881 tcgttaaaat gcggtgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga
     5941 atgttccata ccgttgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca
     6001 tttagtgggt cccagaagaa aatcaataca tcaattttcc cttctgagat caatgcgcca
     6061 acctgctggt caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg
     6121 cgggaaatta agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt
     6181 tgatgccgtt ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca
     6241 atatgtttcc gcgaaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa
     6301 ctttcctaca gattactgta agtacttatc gctgcaagat aaagactgaa aaagcctgcg
     6361 cacaggcaca aaaatctcag gaggatggtt gtttttccgc ccattgcagg aaagtatttc
     6421 gcgtttgtgg gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag
     6481 actgcgtcgg aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca
     6541 tcatcgtggc aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct
     6601 cgacctcata atcaatcgtt tttgcagttg aggtaatggc gagaatatcc agctttaccg
     6661 gaatcggcgt cgcatcacca tccaacaatt caaggcgcgg catcaaaatg gttagcttcc
     6721 cgctcattct ccaggcgagg caggcgaaaa ttgacctggt tgatgagctg ggtattaaaa
     6781 ctaaccacca gtggagggga gatatagagc cgttcttcac tattggaaag atgaatcgtc
     6841 ttctcaacgc gaaacactaa ctgatgcggt ccattgtcca gttcaatgct gtccgcaccg
     6901 cgcagcagag agctggaaac cttcttaccg tcgagtacca gaagatcgac atcagtagaa
     6961 agccgcagcg tggtggcaaa aacggatacc ggcaaacata atgcaatcaa ggtggtcacg
     7021 atgccggttt tcatagatag ctcctgttaa aagaccgcct gaataataat gtgtcaaaat
     7081 ttttctttaa tcatacttac taacatatag acatatttcc cgcttttgct ctcattcatt
     7141 cgtattagct gcatggttgg catgtcggct ttgtcgtaca ctttgttaaa cagtctggag
     7201 gaaataatga aagaaaccga tattgcaggc attttaacgt ccactcacac cattgcgctg
     7261 gtgggggcaa gcgacaaacc cgatcgtccc agctatcgcg tgatgaaata tctactcgac
     7321 caggggtatc acgtcattcc tgtttcgcca aaagttgccg gcaaaacact gctgggccag
     7381 caggggtatg gcacgctggc agacgttcca gaaaaagtcg atatggtcga tgttttccgt
     7441 aattcagaag cggcgtgggg cgtggcgcag gaagctatcg ccattggcgc gaaaaccctc
     7501 tggatgcaac tgggggtgat taatgagcaa gcggcagtgc tggcgcgtga tgcagggcta
     7561 aacgtggtga tggatcgctg cccggcgatt gagatccctc gcctgggcct ggccaaataa
     7621 aaaatccccg gaaggcaaaa accttccggg gatttgttca gggattagtt acgcagacgc
     7681 ggggcctgga gttgtttgcg gatggtctgc gccagctcat ccatggatgg ctgttcagga
     7741 tgttcatcct gcaattcgct acttagctgg gcttcagcca ggtaggtatg aaccggtagg
     7801 ccgttatcat cctccatcac cacgtgatac cacggagcgg cgcgaagctc gtcattcacc
     7861 gccagctcat caggcgatgg ttcagagagc gaataaaccg ggtcgatatc cacgaccact
     7921 ccgagataac ctaacaggga atggcggacc tgctggccga taccgaattt gctggcaatc
     7981 atagtcacct cccgggaaat ctttaccttt gatataaggg taaaattcca catttcaagt
     8041 tacatgacgc gacaggcaaa ccctttcaga tatagccctt ccgggtaggt agcgatcacc
     8101 ggatgatcgg ctgcctgacg gaactgctct ataaattgta catcacgacc ggcatcaatt
     8161 gcggcatccg cgatgatttt ctgaaataaa tcgctggtca tcagaccgga acaggagaaa
     8221 gtcaggagaa taccgccttc attcagcaac tgaatcgcca gcatgttgat gtctttataa
     8281 ccacgacacg cgcccatcaa ctggctttta ttctcaacaa acttcggcgg gtccatcacg
     8341 ataacatcaa atttttcacc gcgatcgcga taagtacgca gcaatttaaa gacatcatca
     8401 cggacaaact ctgccttgct cagatccagt ttgttcagct caacgttctg ccgtgcaata
     8461 tccagcgctt cctgggaggt atcaacgctg acaacctggc tgcaaccgcc catcaatgcc
     8521 gatacggcga aaccaccggt ataggagaaa cagttaagta cgcgtttatt ttcgacgtag
     8581 cggcgggtag ccaggcggct gtcacgctgg tccaggtagt agcccgtttt gtgtccgtgc
     8641 tgaatatcca ccagcagttt catgccgtgt tcttcaatcg gcagcagggc aggcggcaac
     8701 tcgccggtga tggggccctg agtcagctcc atcccttctt ttttacgtac cgcgacgtcg
     8761 ctgcgatcgt aaatagcaca tgccgggtac agcgtttgca gggcactaat taatgccgcg
     8821 cgctgatact ctgcgccagc actcagcagt tgcagcacca gaaaattacc gaaacgatcg
     8881 atagtaatac ccggcaggcc atcagattct ccggcgatta aacgatagct gtcgaggcca
     8941 tctttttgcg ccagccagtc acgccatttt tgtgcttgct gtaaacggcg ggtaaaaaaa
     9001 gcaatgtcga tagactctga cgggtcaaac gtccagacgc gcgcccggat ttgcgaagct
     9061 ggcgaataag cgccgcgtgc taaccatttt ccctgatgat caacaatatc gatggtttca
     9121 ccgaggctgg ctttaccttc catgcgggca acggccccgg aaaagaccca cggatggcga
     9181 cgaagtaatg atttttcgcg ccctttggct aacactaaac gtacactcat aatttacttt
     9241 tctgtcgatg caaagaaatg ggcgtcattg tccggagttt tccatggatt tgcaacgcgt
     9301 ccattaagga taaggagagc agcaatgtcg aaagtctgca taattgcttg ggtttacggg
     9361 cgagttcagg gcgtaggatt ccgctacacc acgcagtacg aagcgaaaaa actgggacta
     9421 accgggtacg ccaaaaatct tgacgatggc agcgtggaag tggttgcctg cggtgacgaa
     9481 gggcaggtgg aaaaattaat gcagtggtta aaaagtggcg gcccgcgttc agcgcgtgtg
     9541 gaacgggtgc ttagcgagcc gcatcatccc tccggggagt taacggattt tcgtattcgt
     9601 taaatgcatt ttaccggttt aggcaggcca gcaattttgg tggcttgctt tgccggacct
     9661 ttcgggaaca gtcggtacag atagcggctg ttaccttttt cttcgccaaa tttattcgcc
     9721 atcgctttta ccagcatacg aatcgccgga gaagtattga attccagata gaaatcacgc
     9781 acaaaacgca ccacttccca gtgttctggc gacagcgcaa tcccttcgtt ctctgcaatc
     9841 accaccgcca gtggttcact ccactggctg ctttctttga gatagccttc ggtatccgtt
     9901 tctatctctt taccttcgaa gatcagcata attctttact accttaatca gactggcggc
     9961 agtgtaacaa aaaacaaagc cccgcataaa gcgaggctcg gtaagtaacc ctgggcgaga
    10021 ttaatcgcgg ctggcgaagc ccagaatgct cagcaggctg acgaagatgt tgtacagcga
    10081 aacatacagg ctaaccgtgg cacgaatata gttcgtttca ccgccatgaa tgatgttgct
    10141 ggtttcaaac aaaatagcgc cagaggagat cagaatgaag accgcgctga tcgccagatg
    10201 cagagcaggc agttgcagga agatattagc aaccatacca atcagcacca ccacaatacc
    10261 cgccatcagc ataccgccga ggaacgacat atctttgcgg gtggtcagca catacgcaga
    10321 gcagcagaag aacaccaacg ccgttccgcc cagtgccata gcgattacgt cacccattcc
    10381 ggcagacaga taggtgttca gaattggtcc gaggatataa cccagaaaac cggtaaaggc
    10441 gaatgcggag ataatcccgg tcggcttatt cgccgttttg taggtcagga acatcaaacc
    10501 atacataccc accagcgtca gaatcagacc cggagatggc agcatcagca ccgtactggc
    10561 agttgcggta atcgccgaaa aggccagcgt caggctcagc agaaaatagg tattacgcag
    10621 caccttatgg gtgctaagca gcgatgtacg gtcatgtgaa gaactaacta tacgatccat
    10681 tgagtcactc tctatgacag atgtaattaa ttaagcagca taatgataat gtgggaaggt
    10741 acacagaagt tttacccatc tttacgcatt tgatctgaaa caagtttcac agcggagtct
    10801 caggtcatta ggcagtgata taacgccctg agaatttcga caggcaaaag aaaaaggggt
    10861 tagcatttag ctaacccctt atcttatttg gcggaagcgc agagattcga actctggaac
    10921 cctttcgggt cgccggtttt caagaccggt gccttcaacc gctcggccac acttccggaa
    10981 tgacgcgcac tataaacatc ccgatgcggc gtgtaaaccc ctaatttgtt tgtttgcctg
    11041 aaaaacagcc aaaagtgcat taatagcgtg aaataacagc agattgatca tttcatcacc
    11101 atgaattcct tctcttttac tcgtttagca accggctaaa catccccacc gcccggtaaa
    11161 aagaaaaata ggtccatttt tatcgctaaa agataaatcc acacagtttg tattgttttg
    11221 tgcaaaagtt tcactacgct ttattaacaa tactttctgg cgacgtgcgc cagtgcagaa
    11281 ggatgagctt tcgttttcag catctcacgt gaagcgatgg tttgccttgc tacagggacg
    11341 tcgcttgccg accatgtgcg cccggtgtcc tgccggtgtc gcaaggagaa gagacgtgcg
    11401 atatgaataa tgaggaaaca ttttaccagg ccatgcggcg tcagggcgtt acccggcgca
    11461 gctttctcaa atattgtagt ctggctgcca cgtcgctggg attaggcgcg ggaatggcac
    11521 caaagattgc ctgggcgctg gagaataaac cgcgcattcc ggtggtgtgg atccacggtc
    11581 tggaatgcac ctgctgtacc gaatctttta tccgctccgc tcacccactg gcgaaggacg
    11641 tcatcctttc cctgatttcc ctcgattacg acgatacttt gatggctgcc gccggaaccc
    11701 aggcggaaga agtctttgaa gacatcatca cgcaatacaa tggcaaatat atcctcgccg
    11761 ttgaaggtaa tccgccgctg ggcgagcagg ggatgttctg tatcagcagc ggtcgaccgt
    11821 ttattgagaa actcaaacgt gccgctgccg gagccagtgc gattatcgcc tggggaacct
    11881 gcgcgtcctg gggctgcgtg caggccgcgc gacccaatcc gacgcaggca acgcctatcg
    11941 acaaagtcat caccgacaaa cctattatca aagtacctgg ctgcccgccg atcccggatg
    12001 tgatgagcgc catcattact tacatggtga cctttgatcg cttgccagat gtcgacagaa
    12061 tgggccgtcc gttgatgttc tatggtcagc gaatccacga taaatgctat cgccgcgctc
    12121 acttcgacgc cggagagttc gtgcagagtt gggatgatga cgctgcccgc aaaggttact
    12181 gcctgtacaa aatgggctgc aaagggccta ccacctataa cgcctgttcc tccacacgct
    12241 ggaatgatgg cgtttctttc ccaatccagt ctggtcacgg ctgcctgggt tgtgcggaaa
    12301 atggtttctg ggatcgcggt tcgttctaca gccgcgtggt cgatattcca caaatgggta
    12361 cccattccac cgccgatacc gtcggtttaa ccgcacttgg cgtggtggca gcggctgttg
    12421 gtgtgcacgc agtcgccagc gccgttgacc agcgcagacg tcataaccag caacctacag
    12481 aaaccgaaca tcagccaggc aatgaggata aacaggcatg agcactcagt acgaaactca
    12541 gggatacacc atcaacaatg ccggacgccg cctggtggtc gacccgatta cgcgcattga
    12601 aggccacatg cgctgcgaag tgaatattaa cgatcagaat gtgatcacca atgccgtctc
    12661 ctgcggcacc atgtttcgcg ggctggagat cattctgcaa gggcgtgacc cgcgcgatgc
    12721 gtgggcgttc gttgaacgta tctgcggcgt ctgtactggc gtacacgccc tggcttcggt
    12781 ttacgccatc gaagatgcca tcggtattaa agtgccggac aacgccaata tcatccgcaa
    12841 cattatgctg gcaacgctct ggtgccacga tcatctggtg cacttctatc agcttgccgg
    12901 gatggactgg atcgatgtgt tagatgcgct gaaagccgac ccgcggaaaa cctccgaact
    12961 ggcgcaaagc ctctcctcat ggccgaaatc atcccctggc tatttcttcg acgtacaaaa
    13021 ccgcctgaag aaattcgttg aaggcgggca gttggggatc ttccgcaatg gctactgggg
    13081 gcacccgcag tacaaactgc cgccagaagc caacctgatg ggctttgccc actatctcga
    13141 agctctcgat ttccagcgtg aaattgtcaa aatccacgcg gtctttggtg gtaaaaaccc
    13201 gcatccaaac tggattgtcg gcgggatgcc ttgcgccatt aacattgacg aaagcggcgc
    13261 agtcggggcg gtcaatatgg aacgcctgaa cctggtgcag tcgatcatta cccgtacggt
    13321 ggacttcatt aacaacgtga tgatccccga cgccttagcc atcggccagt tcaacaaacc
    13381 gtggagcgaa atcggcacgg gtctttctga taaatgtgtc ctcagctacg gcgcattccc
    13441 ggatattgcc aacgactttg gcgagaaaag tctgctgatg cctggcggcg cggtgattaa
    13501 cggcgacttc aacaatgtgc tgccagtgga tttggttgat ccgcagcagg tgcaggagtt
    13561 tgtcgaccac gcctggtatc gttatcccaa cgatcaggtc gggcgtcatc cgttcgatgg
    13621 catcaccgat ccgtggtaca accccggcga tgtcaaaggc agcgatacca acattcagca
    13681 gctgaatgaa caggaacgct actcgtggat caaagcgcca cgctggcgcg gtaacgcgat
    13741 ggaagtgggg ccgctggcac gcacgttaat cgcttatcac aaaggcgatg ctgcgaccgt
    13801 tgagtcggtc gatcgtatga tgtcggcgtt gaaactgccg ctttccggca tccagtcaac
    13861 gttgggccgc attttgtgtc gcgcgcacga agcgcagtgg gccgcaggta agttgcagta
    13921 tttcttcgat aaactgatga ccaacctgaa aaacggcaat cttgccaccg cttccacgga
    13981 aaaatgggaa ccagcaacct ggccgacaga gtgccgtggt gtcggtttta ccgaagcgcc
    14041 gcgcggggcg ttaggccact gggctgccat tcgcgatggc aagattgatc tctaccagtg
    14101 cgtggtgccg accacctgga acgccagccc gcgcgatccc aaagggcaga ttggcgctta
    14161 tgaagcggcg ctgatgaaca ccaaaatggc gatccccgag caaccgctgg agatcctgcg
    14221 tactctgcac agctttgacc cgtgcctcgc ctgttcaaca cacgtgctgg gcgacgacgg
    14281 tagcgagctt atctccgtgc aggtgcgtta acagcgaagg agaatcatca tgcaacagaa
    14341 aagcgacaac gttgtcagtc actatgtctt tgaagcaccc gtgcggatat ggcactggct
    14401 gacagtgtta tgcatggcgg tgttgatggt caccggttac tttatcggca agccgctacc
    14461 ttccgtcagc ggcgaggcga cgtatctgtt ctatatgggc tacatcaggt taattcactt
    14521 cagcgccggg atgattttta ccgtggtctt gctgatgcgg atctactggg cttttgttgg
    14581 caatcgttac tcccgcgagc tgtttatcgt gccggtatgg cgtaaaagct ggtggcaggg
    14641 cgtgtggtat gaaatccgct ggtatctgtt tctggcaaaa cgtccgagtg ccgatatagg
    14701 ccataacccc atcgcccagg cggcgatgtt cggctatttc ctgatgtcag tttttatgat
    14761 catcaccggc tttgcgctgt acagcgaaca cagccagtac gctatttttg cgccgttccg
    14821 ttatgtggtg gaatttttct actggacggg tggcaactca atggacattc acagctggca
    14881 tcggctgggg atgtggctga ttggcgcgtt tgtgatcggt catgtctaca tggcgctgcg
    14941 tgaagacatc atgtccgacg acacggtgat ctccactatg gtcaacggct accgtagcca
    15001 caaatttggc aaaataagta acaaggagcg ttcatgagcg agcaacgcgt ggtggtaatg
    15061 gggctgggca acctgctgtg ggccgatgaa ggcttcggcg tgcgggtggc ggaacggctg
    15121 tatgcccatt accactggcc agaggatgtg gagattgtcg atggcggcac ccaaggactg
    15181 aacttgctgg ggtatgtcga aagcgccagt catctgttga ttctcgatgc cattgattac
    15241 gggctggaac ccggaacgct acgaacgtat gccggagaac gcattccggc ttatctcagc
    15301 gcgaagaaaa tgagcctgca ccagaacagt ttctccgaag tgctggcgct ggcagatatc
    15361 cgcggacatc tgccagcata tattgccctc gtcggtctgc aacccgcgat gctcgacgac
    15421 tacggcggca gcctgagcga actggcgcgg gagcaactgc ccgctgcgga acaggcggcg
    15481 ctggcgcagc tggctgcgtg gggaattgtg ccgcaaccgg ctaatgaatc gcgctgtctc
    15541 aattatgact gtctgtcgat ggaaaattac gaaggcgttc gcttgcgtca gtacaggatg
    15601 aggctggagg aacagggatg agcaacgaca caccatttaa tgcgttgtgg caacgaatgc
    15661 tggcacgtgg ctggacgccg gtcagtgaat gccgtcttga cgactggctt acgcaagcgc
    15721 cagacggcgt ggtgttatta agcagtgacc cgaaacgcac gccagaagtc agcgataacc
    15781 cggtaatgat tggcgaatta ctgcgcgaat ttcccgacta tacatggcag gtagcgattg
    15841 ctgatcttga gcagagcgaa gccatcggcg atcgctttgg cgtctttcgc tttcccgcca
    15901 cattagtgtt taccggcgga aactatcgcg gcgtgctgaa tggtattcac ccgtgggcgg
    15961 aactgataaa cctgatgcgc gggcttgtcg aaccgcaaca ggagcgtgct tcatgagcga
    16021 aacttttttc catctgctgg ggccaggaac gcaaccgaac gatgacagtt tcagcatgaa
    16081 tccactgccg atcacctgtc gggtgaatgg tgaaccgagt atggcggccc tggagcaatg
    16141 tgctcacagc ccgcaggtga ttgcgctgtt aaacgagtta caacatcaac taagcgaacg
    16201 ccagccgccg ctgggtgagg tactggcagt cgatctgtta aatctcaacg ccgacgatcg
    16261 tcactttatc aatacgcttc tcggggaagg ggaagtgtca gtgcgcattc agcaggctga
    16321 cgacagtgaa agtgaaattc aggaggcgat cttctgcgga ttatggcggg tacgcagacg
    16381 tcacggcgaa cagttgctgg aagacaaact ggaggctggc tgcgcaccgc tggcgttgtg
    16441 gcaggcggca acgcaaaacg tcttgccgac agattcgctg ttaccgccgc ccattgatgg
    16501 cctgatgaat ggcctaccgt tggcctatga attactggca catgtacgta accccgacgc
    16561 gcagccgcac agcattaata tgacgcaatt acccatcagc gaagctgacc ggctttttct
    16621 ctcacgtctc tgtgggccgg gaaatattca gattcgtacc attggctatg gcgagagcta
    16681 tatcaacgcc acggggttac gccatgtctg gcatttacgc tgtacggaca ccttaaaagg
    16741 cccgttactg gaaagttatg aaatctgccc aataccggaa gtggtgctgg cagcgccaga
    16801 agatttggtc gactctgcgc agcggcttag cgaggtatgc cagtggctgg cggagggggc
    16861 accgacgtaa aaagacggta agtatcgctt tcaggcttat gaatatcgca atcggcgaat
    16921 acctctggtc gtagagtttc aggataaaga gggagatcta ccattaacgg gttatttttc
    16981 tctcttcgcc tacaggagtg cgcatgtggg atgtcattga tttatcgcgc tggcagtttg
    17041 ctctgaccgc gctgtatcac tttttatttg taccccttac cctggggctg atttttttgc
    17101 tggctatcat ggaaaccatt tacgtggtca ccggcaaaac aatctaccgc gatatgacgc
    17161 gcttctgggg taagctcttc ggtatcaact ttgctcttgg cgtggctacc ggcctgacca
    17221 tggagtttca gtttggtact aactggtcat tctattccaa ctatgtgggc gatatttttg
    17281 gcgcaccgct ggcgatggaa gcattaatgg ccttcttcct cgaatccacc tttgtcgggc
    17341 tgttcttctt cggctggcag cggctgaata aataccagca cctgctggtg acgtggctgg
    17401 tggcgttcgg ttcaaatctc tctgcgttgt ggatattgaa tgccaacggt tggatgcaac
    17461 acccgactgg tgcgcatttt gatatcgaca ccctgcgtat ggagatgacc agtttcagcg
    17521 agctggtctt taacccggtc agccaggtga aatttgtgca caccgtaatg gcgggatacg
    17581 tgaccggggc tatgtttatt atggcgatca gcgcctggta tttactgcgc ggacgggagc
    17641 gcgatgtcgc attacgctcg tttgccatcg gttccgtctt cggtactctg gtgattatcg
    17701 gtacgctgca actcggagac agttctgcgt atgaagtcgc gcaagtacaa ccggtaaaac
    17761 tggcggcgat ggaaggggag tggcaaacgg aacctgcacc tgcaccgttc catgtggttg
    17821 cctggccgga acaggatcaa gagcgtaacg cctttgccat caaaattccc gcgctgctag
    17881 gaatcctcgc cactcattca ttagataaac ccgtgccggg tctgaagaat ttgatggctg
    17941 aaacctaccc acgcttgcaa cgcggacgta tggcgtggct gttaatgcag gaaatatcgc
    18001 aaggcaatcg tgagccgcat gtgttgcagg cattccggga gctggaaggc gacctgggtt
    18061 acggcatgtt gctctcccgc tatgcgccgg atatgaatca tgtcacagcc gcacagtacc
    18121 agacggcgat gcgtggcgcg atacctcagg ttgcgccggt attctggagt ttccgcatca
    18181 tggtgggctg tggttccctg ctgttactgg tgatgctgat tgcgcttgtc cagacgctgc
    18241 gtggcaaaat cgaccagcat cgctgggtgc tgaaaatggc gctctggagt ttgccactgc
    18301 cgtggattgc gattgaagcc ggatggttta tgaccgagtt tggtcgtcag ccgtgggcga
    18361 tacaggacat cttaccgaca tactccgcgc actccgcctt aaccacagga caactggctt
    18421 tctcactgat catgatcgta gggctttaca ccctgttctt aatcgccgaa gtctacctga
    18481 tgcagaaata tgcccgtctg gggccgagcg cgatgcagag tgaacaaccg acgcagcaac
    18541 aggggtaaag gagaaaatca tgtttgatta tgaaacattg cgcttcatct ggtggctgct
    18601 gattggcgtg atcctggtgg tctttatgat ctccgacgga tttgacatgg ggatcggctg
    18661 tctgctgccg ctggtggcgc gtaatgatga tgaacgtcgg atagtgataa acagcgttgg
    18721 cgcgcactgg gaaggcaatc aggtctggtt gatcctcgca ggtggggcat tatttgccgc
    18781 ctggcccaga gtgtatgcag cggcgttttc cggcttttat gtggcgatga ttctggtgct
    18841 gtgctcgctg ttcttccgcc cgctggcctt tgattatcgc ggaaaaatcg ccgatgcacg
    18901 ctggcgtaaa atgtgggatg ccggactggt catcggcagt ctggtgccgc cggtagtctt
    18961 cggtattgca ttcggcaact tgctgctcgg tgtgccgttt gccttcacac cgcaattacg
    19021 cgtggaatat ctcggcagct tctggcaact gctgacgcca ttccctttat tgtgtggatt
    19081 gctcagtctt gggatggtga ttttgcaagg tggcgtctgg ttacaactga aaactgttgg
    19141 tgtgattcat ctgcgttcac agctggcgac caaacgcgcc gcactactgg tgatgctgtg
    19201 ctttttgctg gcgggttact ggctgtgggt tggtattgat ggctttgtac tgctcgccca
    19261 ggatgccaac ggtccttcca atccgttaat gaaactggtg gcagtgctac ctggtgcctg
    19321 gatgaataat ttcgtcgaat cgcccgtttt gtggatcttc ccgctgctgg ggttcttctg
    19381 cccattgctg acggtgatgg cgatttatcg tggtcgcccg ggttggggat ttttgatggc
    19441 gtcattgatg caatttggcg tgattttcac ggcaggcatc acgctgttcc cctttgtcat
    19501 gccgtcaagc gtgagtccga tctccagcct gacgttgtgg gacagcactt ccagtcagct
    19561 gacgctgagc attatgttgg taatcgtgct gatatttttg cccattgtgt tgctctacac
    19621 tctctggagc tactacaaaa tgtgggggcg catgacgaca gaaactctcc gccgtaacga
    19681 aaacgagctg tactaaggag caggaacaat gtggtattta ctttggttcg tcggcatttt
    19741 gttgatgtgt tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta
    19801 acgaaggtaa gcctgatgag gcgcattagc gtcgcatcag gcaatcaata atgtcggata
    19861 tgaaaagcgg aaacatatcg atgaaagcga tcttgatccc attcttatct cttctgattc
    19921 cgttaacacc gaaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtgtgg
    19981 tgattgtcag tcgtcacggt gtgcgtgcac caaccaaggc cacacaactg atgcaggatg
    20041 tcacccctga cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg
    20101 gtgagttaat cgcttatctc ggacattacc aacgccagcg tctggtggcc gacggattgc
    20161 tggcaaaaaa gggctgcccg cagccaggtc aggtcgcggt tattgctgat gtcgacgaac
    20221 gtacccgtaa aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg
    20281 tacataccca ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggcg
    20341 tttgccaact ggataacgcg aacgtgactg acgcgatcct cagtagggca ggaggggcaa
    20401 ttgctgactt tgccgggcat cggcaaacgg cgtttcgcga actggaacgg gtgcttaatt
    20461 tcccgcaatc aaacttgtgc cttaaccgta agaaacagga cgaaagctgt tcattaacgc
    20521 aggcattacc atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa
    20581 gcctcgcatc aatgctgacg gagatatttc tcctgcaaca agcacaggga atgccggagc
    20641 cggggtgggg aaggatcacc gattcacacc agtggaacac cttgctaagt ttacataacg
    20701 cgcaatttta tttactacaa cgcacgccag aggttgcccg cagtcgcgcc accccgttat
    20761 tggatttgat catggcagcg ttgacgcccc atccatcgca aaaacaggcg tatggtgtga
    20821 cattacccac ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg
    20881 gcgcactgga gctcaactgg acacttccag gtcagccgga taacacgccg ccaggtggtg
    20941 aactggtgtt tgaacgctgg cgtcggctca gcgataacag ccagtggatt caggtttcgc
    21001 tggtcttcca gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc
    21061 ccggagaggt gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt
    21121 cgttggccgg ttttacgcaa atcgtgaatg aagcacgcat accagcgtgc gctctccatc
    21181 aagacaaata aacatttgcc gctgttggtc cacgcaggcc atttacgcgg caaaattcca
    21241 cgcgtaatcc tggtataagc acttctgcgt cgcggggagt gaatgcggaa atatggacct
    21301 ggacttcttt acgaccgtcg gaggggataa tgaatccttt gccgcttttg cgatcaaagg
    21361 ttttgacaat tcctgtcatt ttacgggaca aacaaattcc ttactgaaaa tactgcgctg
    21421 cactatacgg ggttaataaa ataaagccag cgatatttaa gaccgccgga cggctaaaat
    21481 aaaatttgct taatctcaat tatcatgcgt taatagctgc gtcggtttga aagacagaca
    21541 gcatacaaag tagtttacta aagcagttct cattatcagg cattatcccc ttcttttgag
    21601 tctctctcct gaacactaag tagtttctgt attaaagccc tgtttgccga aaggcccaaa
    21661 atgaaggaag taaaatatgt ctaataaaat gactggttta gtaaaatggt ttaacgcaga
    21721 taaaggtttt ggctttatca ctcctgatga tggcagcaaa gacgttttcg tccatttcac
    21781 tgccatccag agcaatgaat tccgcacgct gaacgaaaat cagaaagttg aattttctat
    21841 tgagcagggg caacgtggtc ccgcggcagc gaacgttgtt acgctctaag gttgccgtta
    21901 ttactcaaca tctccatttc cgctgtccat gttgccatgg ttcacagtac cgcacatcgg
    21961 cattcgatgt gacggagcga aatcctttgg gtgcaaagtg cattttttgt aaatcgacga
    22021 tgatcacctt tgataacgtc gcgttgcaaa tacgcactga ccatgcgccg ctggatttca
    22081 caaaataata tcaggctccc tcgtggagcc ttttttatat ctgccttatt tttcttcaac
    22141 gctgtatgta tagtaagcga taacctgttg attattgaat ctttcgggga gatggcttat
    22201 aacatttctt acctgatcag ggtaccggga accaacacct tactggcgtg ttgctgtctt
    22261 ttaagaccag aagaggttaa cagtgaatat tgaagagtta aaaaaacaag ccgaaacgga
    22321 aatcgccgac tttatcgcgc aaaaaatcgc cgagctgaac aagaatacag ggaaagaagt
    22381 ctctgaaatt cgcttcaccg cacgagaaaa aatgaccggg cttgaaagtt atgatgtcaa
    22441 aatcaaaata atgtgatttt gtgaacatca ccccgtgcga ggtgatgttc cgcttgttgc
    22501 taatttagtg accaatcatt ggcgattgcg gaattaagcg ttggtacaat tcctccggca
    22561 ccgggctttg ccatactccc gcatacattg cgtaaccaat cgccgcaaac ataatcccca
    22621 taaccagtag cgtcattaac cagccagaca acgcaaaggc ttttttattt gccgcaggtt
    22681 tttgcagtga aaaggtcaat gttgaggcta ccggacatga ctctacgcaa gtcatacagc
    22741 cggtacattc cactgttcgt acctgaatta atttatcgac cgggatccgt gatgggcaac
    22801 tttttgcgca tttgccacaa tcgatacaac tttcggcatt gcgacgaatc ttaaacggcg
    22861 ataatagcga aaccacgccc atcagcgcgc catatgggca aagataacga caccaggcat
    22921 ggcgaataaa caggctggca atcagcaaaa cgatcatgct gattaatgtc gcggtcccca
    22981 tatgacgaaa gaaatcgagc attttaacgt ccatcaccac gctgtagggc gacaacataa
    23041 aatagtgaat cgcctgagcg ggcatcaata acgcgatata gagaaaaaaa ctcaacagca
    23101 aatacttcac gccgcgcaga ggaatatcca gccagcgggg aaggacaaat tgccgaccaa
    23161 acagtttgtt accgagatcg ccgattaatt ccgaaagcgt accaaccggg cataaccatg
    23221 aacaaaaggc ctttttgagt aatagactga tgacgataaa agcgaccaat aacagcatcg
    23281 cggcggcgtg gacggacggt aactgacctg ttgcaaggct atatttcaga ttcatcagac
    23341 cggcaatcgg tagccagcct tcgatacctc ccggtctggt gataaatgtc gtactacctg
    23401 ccgtttcgta atagcgcacc caataccaga acgtgatggc aatataaata ttaattgcca
    23461 acagtaataa ttgcgtcgtt ttacgccagg tcgtggcatt gcgccagtca ttccacggta
    23521 atttgccgcc cgtcgtgcct ggccgccgct gccagcgggt tctttttttc tctgccatga
    23581 ttttgccagt ccgttaagtt gcataccaaa tgccactatt ctagttgttc ttaactggct
    23641 gatattgatt caaatcgcgt tcaggttttt cttattaacc atgcttccag agcggcaaca
    23701 ctgcgtgtaa tttcttcgtg tggaaggggg gcagataatg gctgctgctc cagttgtgcg
    23761 cattgctggc tggcgctacg cattcccaga ctcgagcaac tgctttttag ctgatgtgcg
    23821 gcacgcttta ttttctcgtt atcctgactg gcgcgggcaa tgtcgatttc atcgagaagc
    23881 ggcagggcat gttgtttaaa taatatcagc cattcgtgga tcttctccgc ccccattaaa
    23941 tgagcatctt cattgagttg cgatacatcc agcggttgat cgttattgac ttgcagttgg
    24001 agatagtgcg ccagtaattg accgagcact tcacgcggca ccggtttagg gataatcccg
    24061 cgaaatagcg aactggtacg ctgtcgcagt gtttcgtcaa tgacatgggc gctaaagcca
    24121 atcaaaacca gcgacggata ttgctgtgcc agttgtcggg caagcgtaat gccgtcgata
    24181 tccggcagat caaaatccac cagtgcgaca gcaaacggtt cactattttg cagtgtctct
    24241 aaagcctgcg cggcatttcc tacggcaacg acctgcgccc cactgctgtt caacatctca
    24301 acggtaatcc gctgggttag cgggttatct tcaattaaca gcaaacgtaa accgtccaga
    24361 cgcaccgctt gattgactgt ttttggcacg ggtgcagtgg caatacgtaa cggcaagcgt
    24421 aaacaaaaac agcttccaac ctccggcgtg ctggtggcgc tcagttcgcc acccatcgcc
    24481 tgggccagac ggctactgat agtcagcccc agcccggtgc cgccgcgttt gccgctaacc
    24541 tgaacaaatg gctggaagat ttcagccagt ttcgcgggat caataccgca gccgctgtct
    24601 tccacttcga ccagccattg ttcgccatca gtgcgactac gcagaataat gtgcccttcg
    24661 tcggtgaaac gcagggcgtt gctcaacagg ttggtaatga cctgacgaat acgccgcgga
    24721 tcgcccatta acgcagtcgg tacatcgtcg gcaatttctg ttgccaggcg aatcgggcga
    24781 cctttcaccc ttccgctcat taattgcagg gtactttcca gcagcgggcg cggttcaaag
    24841 ggttcatcgc tgaccgaaac attcttgccg cctgcttcga tagccgaata atcgagaata
    24901 tcgttgagga tggtcagcaa cgattcacca gagtcggtaa tcgcccgcaa atcatcgcgc
    24961 tgggcgttaa gtgcggggtt atctgccagc agttgcgcgg tgccgagaat accgtacagc
    25021 ggtgtgcgga tctcatggct catcgccgcc agaaacgccg attttgcctg gctggctttt
    25081 tctgcttccg cccgtgcctg tcggtgttct atcaccagtt cctgcaattc agctgtacgc
    25141 gctttgacct gcgccgccag ctgttcgcgg tggcgattca gtgcatgaac actgctgcga
    25201 aacgcatcca tcagccgccc gatggtatcc agttcccgta cgccagcggt ttccgggaaa
    25261 ggggagtcga tatcaccatc cagcaagcgt tgcagcgcct gcgtttgttc tgcaagaggg
    25321 cgcgtgactg agcgataaac cacgcgccag aggatcagaa tcagtgcgca aagagaaact
    25381 aatcccagca ataacaggct gtattgcccg cgtgcactgg ctttttccag atgtgccagc
    25441 ccgtgctgat tacgcagctc aatggtgtcg accagctgac tgacttcgct actaaactgc
    25501 gcgaattggg cgatgttatt ttgggcgaga gtttgcaggt gattgctgat ttcactgtcc
    25561 tgctgataca gcgccagcaa atcgctatat tggctaacgg tagttaacgt tgttgctacc
    25621 tgagtacgaa cacccggatc ttcaatgcgt atttgccgac gttgcagaat tttcaccgca
    25681 ttattgagct gcttttccag cgttggcgca tttttctgga tctgttccag ccccagattc
    25741 atcaccattt gctgcacccg cagggcgcta aggcgcagtt cattcatctg gttaacatac
    25801 tcaagatcga tatcaatcag ccgatcgagt gcactttctg cggcctgacg ctgatgttgt
    25861 tcgatcaaat cgtaaatccc ggcctgggtc gctccggcgg aggttgccgc attattcgcc
    25921 tgaccttgcg ccaggcgtgc gatttcatcg gcggcggcga ctatctgctg actgagttgc
    25981 tgttgttgct ggcgaagttg caaacgctgc cccaccagtt ccccttgctg acgtaacgaa
    26041 cgggagatct cctgctcctg ttgttcaata gcggtggtgt caaaaccttg ttcccgtaac
    26101 gcttgcagca acgcattaat cttcaggctt tgtgcggtga gcattcgccc ctgcgcctgc
    26161 cacatctttt cgttatcggc actggtcagg ttctgcgcgg cgaaaagttc ccaggcgctg
    26221 gcttcgctca actggcgcgc catattcatg gtaggaatca atgcctgagt gttgtctttt
    26281 tccacctggc tgataaagcg caggttgtac catcccacca gggtactggt cagggttaac
    26341 agcgccatca gggcaaagcc catccagagt cttcgggtca gggttaaatt cacggtcggt
    26401 gcactttggg tgaaaaaggt tgagtcgcaa agcggaatgc atctagcata aagccttatt
    26461 attgatgagg ctatcatgcg cgtactgcta ttgttactac tttccctttt catgttatcg
    26521 gcattttcgg ctgataacct gttgcgctgg catgatgcgc agcatttctc ggtgcaagcc
    26581 tctacgccgc taaaagccaa acgcgcatgg aaactgtgcg cgctttatcc cagcctgaaa
    26641 gattcatact ggttatcgtt gaactatggt atgcaggagg ctgctcgccg ctacggtgtg
    26701 gatttaaaag tgctggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
    26761 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac gacgtcattt
    26821 cccgacctgc aaaagcaggt agcaagtctg ccggtaatcg aactggtgaa tgctattgat
    26881 gctccccacg tgaaaagccg cgttggtgtg ccctggtttc agatgggcta tcaaccagga
    26941 cgatatctgg tgcaatggag ccacggtaaa ccactgaacg tgctgttgat gcccggaccc
    27001 gataacgccg ggggcagtaa ggagatggtc gagggttttc gcgcagccat tgccggaagc
    27061 ccggtacgta ttgttgatat tgcgctcggt gataacgata ttgaaatcca gcgtaacctg
    27121 ttgcaggaga tgctggaacg ccatccagaa atcgacgtcg ttgccggaac ggccattgcg
    27181 gcagaggcgg caatggggga agggcgcaac ctgaaaacgc cgcttaccgt ggtgtcgttt
    27241 tatctttcac atcaggtgta tcgcgggttg aagcggggaa gagtgattat ggcagccagc
    27301 gatcaaatgg tctggcaggg ggaactggcg gttgagcagg ctatcaggca attacagggg
    27361 caatcggtgt ctgataatgt cagcccaccg attttagtca tgacgccgaa aaatgccgac
    27421 cgcgaacata tccgccgctc gctgtcacca gggggatttc gtccggttta tttttatcag
    27481 cacacatcag cggctaagaa ataaccttcg ccatgttgtg tcaccagtaa atccgcactg
    27541 agtttatggc gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
    27601 cgacgcgcag agagcatacg cagcagacgt tcacggctga gaatttcgcc cggattagtc
    27661 acaaatgcca ccagcatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
    27721 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
    27781 gtgtgcggtt gcgcctgtcg cgcgaggtcg attcgccaga gcaaattttt cactcgtacc
    27841 accagttcgc gcagctccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
    27901 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
    27961 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gcaaattgat atccagcaga
    28021 atcaaatcca ccggctgatt ctgcataatt tcccgcagcc cggcaccgct cgccgtaacg
    28081 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
    28141 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatatctg
    28201 ttcatattct gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta
    28261 agatcgcttc actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc
    28321 tacgccgacc cagtgctcgt tggtcggtac tggcgctggt cgctactggg attgtgattg
    28381 gcattgcgct gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt
    28441 gtgtcagttg ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc
    28501 agaacgcctc cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag
    28561 gcatggtgaa gcgcaaactg gaagcgagca acgacattta ccagaccttt attgcccact
    28621 ccattgatac acctgagaaa tttgaagcca aacgcgcgga acttgccgag cgtgaatggg
    28681 cgcgaatgaa agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg
    28741 atcatgcgaa gcagcatcct gaagcggcgc gtcagatgaa ggtggcagcg aaagataatc
    28801 aatcctgcat cgactgtcat aaaggtattg cccaccagtt accggatatg agtagtggct
    28861 tccgtaagca gttcgatgag ctgcgcgcca gtgctaatga tagtggtgac acgctgtact
    28921 ccattgatat taagccgatt tatgcggcga aaggcgataa agaagcgtct ggttctctgc
    28981 tgcctgcttc ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatca
    29041 ctggctggac ggaaagtgca ggacgtcagc gtgtactcac ccaattccca ggtaaacgca
    29101 tctttgttgc ctcgattcgt ggtgatgtgc agcagcaggt gaaaacactg gagaaaacca
    29161 ccgttgccga caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag
    29221 gcgacatggt gaacgatatc aaaccgatct gggcttatgc ggattcgctg tacaacggca
    29281 cctgtaacca gtgccacggc gcaccggaaa tatcccactt tgacgctaac ggttggatcg
    29341 gcacgctcaa cggcatgatt ggctttacca gcctcgataa acgtgaagaa cgcaccttgt
    29401 tgaaatatct gcaaatgaat gcgtctgaca ccgcaggtaa agctcacggc gataagaagg
    29461 aagaaaaata atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca
    29521 actcggcggc ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc
    29581 gactgcggcg caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc
    29641 gcactggggg gctatccgcg cgacggtgaa ggatggtcgc tttgtggcag caaaaccgtt
    29701 cgaactggat aaatatccgt cgaaaatgat cgccggattg ccggatcatg tacacaacgc
    29761 ggcgcgtatt cgttacccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga
    29821 cacctcccag cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat
    29881 gttctatgaa gaactggaac gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc
    29941 cagtggttgg caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat
    30001 tgccttgcat ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt
    30061 gatcttgccg cgcgtggttg gctcaatgga agtgtatgaa cagcaaacct cctggccgct
    30121 ggtattgcag aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca
    30181 agcgaactgg tggtgcccgg atcacgatgt ttatgaatat tacgagcagt tgaaagcgaa
    30241 agtcgccgcc ggtgaaattg aggtcatcag catcgatccg gttgttacat ccacccatga
    30301 gtatctgggg cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct
    30361 gcaactggcg ctggcgtata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc
    30421 taactactgt gtaggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca
    30481 gccgaaagat gccgcatggg cagaaaaact gaccggcatt gatgccgaaa ccattcgtgg
    30541 gctggcacgg cagatggcgg cgaacagaac gcagattatt gctggctggt gcgtacagcg
    30601 tatgcagcac ggtgaacagt gggcgtggat gattgtcgtt ctggcggcga tgctggggca
    30661 aattggcctg ccaggtggtg gttttggttt tggctggcac tataacggcg caggcacgcc
    30721 ggggcgtaaa ggcgttattt tgagtggttt ctccggctct acgtcgattc cgcctgttca
    30781 cgacaacagt gactacaaag gctacagcag caccattccg attgcccgtt ttatcgatgc
    30841 gatcctcgaa ccggggaaag tgatcaactg gaacggtaaa tcggtaaaac tgccgccgct
    30901 gaaaatgtgt atttttgccg gaactaaccc gttccatcgc catcagcaga tcaaccgcat
    30961 tattgaaggc tggcgcaagc tggaaacggt tatcgccata gataaccagt ggacctcaac
    31021 ctgccgcttt gccgatatcg tgctgcctgc gaccacgcag tttgagcgta acgatctcga
    31081 ccagtacggc aaccactcca atcgtggcat catcgccatg aaacaggtcg tgccgccgca
    31141 gttcgaggcg cgcaacgact ttgatatttt ccgcgagctg tgccgccgct ttaatcgcga
    31201 agaagccttt accgaagggc tggacgagat gggctggctg aaacgcatct ggcaggaagg
    31261 tgtacaacag ggcaaaggac gcggcgttca tttgccagcg tttgatgact tctggaataa
    31321 caaagagtac gtcgagtttg accatccgca gatgtttgtt cgccaccagg cattccgcga
    31381 agatccggat ctcgaaccgc tgggcacgcc gagtggcctg attgagatct actcaaaaac
    31441 catcgccgac atgaactacg acgattgtca ggggcatccg atgtggtttg agaaaatcga
    31501 acgctcccac ggtgggcctg gctcgcagaa gtatccgttg catctgcaat ctgtgcatcc
    31561 ggatttccga cttcactcgc agttatgtga gtcggaaact ctgcgtcagc aatataccgt
    31621 agcgggtaaa gagccagtat ttattagccc gcaggatgcc agcgcgcgcg gtattcgtca
    31681 cggtgatgtg gtacgcgtct ttaacgttcg tggtcaggtg ttggctggag cagtggtttc
    31741 tgaccgttat gcacccggcg tggcgcgaat tcacgaaggg gcatggtacg atccggacaa
    31801 aggcggcgag ccaggggcgc tgtgcaaata cggtaacccc aacgtgttga ccatcgacat
    31861 cggtacttcg cagctggcgc aggcgaccag tgcgcacact acgctggtgg aaattgagaa
    31921 gtacaacgga acagtggagc aggtgacggc gtttaacggc cccgtggaga tggtggcgca
    31981 gtgcgaatat gttcccgcgt cgcaggtgaa atcatgacca cgctgacagc acaacagatt
    32041 gcctgtgttt acgcctggct ggcgcagttg ttctcccgtg aactggacga tgaacaactg
    32101 acgcaaatcg ccagtgcgca gatggctgaa tggttttcgt tgctgaaaag cgaacctccg
    32161 ctcactgcgg cggtgaatgg gctggaaaac agtattgcca ctctgacagt acgtgacgat
    32221 gcccgtctgg aactggccgc tgacttttgc ggcctgtttc tgatgaccga caaacaagcg
    32281 gcgctgccgt atgcatcggc ctacaaacag gacgagcaag agattaaacg cttgttagtt
    32341 gaggcaggga tggagaccag cggcaatttc aacgaaccgg cagatcatct ggcgatctat
    32401 ctcgagttgt tgagtcatct gcatttttcg ctgggagagg ggaccgttcc tgcgcgaaga
    32461 atcgacggtt tgcggcaaaa aacactgacg gctctgcggg aatggttacc agagtttgct
    32521 gcgcgttgtc gacagtatga ccgctttggt ttttacgcgg cactaagcca gttattgctg
    32581 gtgttagtgg agtgtgacta ccaaaaaaga taatgccgtt tgtaggcctg ataagacgcg
    32641 gcaggcgtcg catcaggcat tag
//
LOCUS       DAZGJT010000047        32253 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZGJT010000047 DAZGJT010000000
VERSION     DAZGJT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.047"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..1833)
                     /gene="ycaO"
                     /locus_tag="ACHKFK_002435"
     CDS             complement(73..1833)
                     /gene="ycaO"
                     /locus_tag="ACHKFK_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO9052755.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTIYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWSFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1963..2655
                     /locus_tag="ACHKFK_002436"
     CDS             1963..2655
                     /locus_tag="ACHKFK_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO9052756.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMNAGEKQLCPRCANP
                     ELTKASRAKRVT"
     gene            2854..3942
                     /gene="serC"
                     /locus_tag="ACHKFK_002437"
     CDS             2854..3942
                     /gene="serC"
                     /locus_tag="ACHKFK_002437"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO9052757.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMSEWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNDSMFNTPPTFAWYLSGLVFKWLKANGGVAAMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4013..5296
                     /gene="aroA"
                     /locus_tag="ACHKFK_002438"
     CDS             4013..5296
                     /gene="aroA"
                     /locus_tag="ACHKFK_002438"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO9052758.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAESARELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRQGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIRGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDFIRITPPEKLKFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQPG"
     gene            5466..6230
                     /gene="ycaL"
                     /locus_tag="ACHKFK_002439"
     CDS             5466..6230
                     /gene="ycaL"
                     /locus_tag="ACHKFK_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO9052759.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGINTDLAISSGLNAYKAATL
                     SDADAKAIANQGCVEMDSGNKVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6403..7086
                     /gene="cmk"
                     /locus_tag="ACHKFK_002440"
     CDS             6403..7086
                     /gene="cmk"
                     /locus_tag="ACHKFK_002440"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO9052760.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7197..8870
                     /gene="rpsA"
                     /locus_tag="ACHKFK_002441"
     CDS             7197..8870
                     /gene="rpsA"
                     /locus_tag="ACHKFK_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO9052761.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9030..9314
                     /gene="ihfB"
                     /locus_tag="ACHKFK_002442"
     CDS             9030..9314
                     /gene="ihfB"
                     /locus_tag="ACHKFK_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO9052762.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9520..11784
                     /locus_tag="ACHKFK_002443"
     CDS             9520..11784
                     /locus_tag="ACHKFK_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO9052763.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGIVTLAFLTFFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEIIATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQVWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRGGQVFLPGCWIHWQMPLIGGICCAAFYAWLTG
                     MQPPALRTVVALAIWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPFILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLTFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCLPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSAHWPLQGSNDKGNNHSCVVK
                     IDDGTNSILLTGDIEAPAEQKMLSRYWQQMQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQITVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11821..13569
                     /gene="msbA"
                     /locus_tag="ACHKFK_002444"
     CDS             11821..13569
                     /gene="msbA"
                     /locus_tag="ACHKFK_002444"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO9052764.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLMWMPLVVIGLMILRGITSYISSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13566..14552
                     /gene="lpxK"
                     /locus_tag="ACHKFK_002445"
     CDS             13566..14552
                     /gene="lpxK"
                     /locus_tag="ACHKFK_002445"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO9052765.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PIPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTGAPVAVSPVRSDAIKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLAQLTSLASGN"
     gene            14589..15821
                     /gene="ycaQ"
                     /locus_tag="ACHKFK_002446"
     CDS             14589..15821
                     /gene="ycaQ"
                     /locus_tag="ACHKFK_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFO9052766.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPHRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHAAEIAQLIQHIHDKGPVRSADFEHSRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHCVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQFFDFSYRLECYIPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTTLQKGLRQAITDFANWQQATRVTLGRC
                     PQGLFTDCRAGWEIDPVA"
     gene            15873..16055
                     /gene="ycaR"
                     /locus_tag="ACHKFK_002447"
     CDS             15873..16055
                     /gene="ycaR"
                     /locus_tag="ACHKFK_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO9052767.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16052..16798
                     /gene="kdsB"
                     /locus_tag="ACHKFK_002448"
     CDS             16052..16798
                     /gene="kdsB"
                     /locus_tag="ACHKFK_002448"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9052768.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLKRVRAEMR"
     gene            16952..17845
                     /locus_tag="ACHKFK_002449"
     CDS             16952..17845
                     /locus_tag="ACHKFK_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO9052769.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSAPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17822..18601)
                     /gene="elyC"
                     /locus_tag="ACHKFK_002450"
     CDS             complement(17822..18601)
                     /gene="elyC"
                     /locus_tag="ACHKFK_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO9052770.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPIADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDHVGYETLGRIWQWLKGSSGEPRQE"
     gene            18737..19522
                     /gene="cmoM"
                     /locus_tag="ACHKFK_002451"
     CDS             18737..19522
                     /gene="cmoM"
                     /locus_tag="ACHKFK_002451"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO9052771.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQILGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19519..20841
                     /gene="mukF"
                     /locus_tag="ACHKFK_002452"
     CDS             19519..20841
                     /gene="mukF"
                     /locus_tag="ACHKFK_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO9052772.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMVLR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20822..21526
                     /gene="mukE"
                     /locus_tag="ACHKFK_002453"
     CDS             20822..21526
                     /gene="mukE"
                     /locus_tag="ACHKFK_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO9052773.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            21526..25986
                     /gene="mukB"
                     /locus_tag="ACHKFK_002454"
     CDS             21526..25986
                     /gene="mukB"
                     /locus_tag="ACHKFK_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO9052774.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEFLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLNQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VIVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            26247..28094
                     /gene="ldtD"
                     /locus_tag="ACHKFK_002455"
     CDS             26247..28094
                     /gene="ldtD"
                     /locus_tag="ACHKFK_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9052775.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGVAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTIETAETKPMDKQTTSRSKPAPVVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNNREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28275..28823
                     /locus_tag="ACHKFK_002456"
     CDS             28275..28823
                     /locus_tag="ACHKFK_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO9052776.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28850..29497
                     /gene="gloC"
                     /locus_tag="ACHKFK_002457"
     CDS             28850..29497
                     /gene="gloC"
                     /locus_tag="ACHKFK_002457"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO9052777.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDA
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFLDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(29549..30739)
                     /gene="aspC"
                     /locus_tag="ACHKFK_002458"
     CDS             complement(29549..30739)
                     /gene="aspC"
                     /locus_tag="ACHKFK_002458"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO9052778.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAALHKELIVASSYSKNFGLYNERVGACTLVAADSETVERAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(30925..32013)
                     /gene="ompF"
                     /locus_tag="ACHKFK_002459"
     CDS             complement(30925..32013)
                     /gene="ompF"
                     /locus_tag="ACHKFK_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO9052779.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEESSLGKGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7901 a         7825 c         8638 g         7889 t
ORIGIN      
        1 gggcattaaa aaaatgcccc gtaatataac tcagacgaat cattaagcct acattgcgta
       61 ggctatttga ttttattttg cccagaatgc tgctttggcg cgttgcagct tttcgtaggc
      121 cttcaacaac gactgatgtg cagcaaacgc gtgcagatcg ctatctactg gttgcaagcc
      181 ataaaacgcc gcttcgccgc tcattgccgc actggcggct tccacggcat ctgcgccgta
      241 catgcgaaca aacgcattca gatattgcag cggctggcga tcttcttcct gcgccagtaa
      301 taacaacgtt tgcaggcagc gataataatt ggcgcgttcc gggctgaata ctgatgagtt
      361 aaactccatc gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc
      421 cagcatggct tttaattcac cgatacgcag agtgtaccag ccgttatccg acccggtcgc
      481 cagacccaac agttcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc
      541 gatgaggttc aggtaatctt ccttttccca ctcgctacct ggtagcgaaa gaatcgtttc
      601 acgtaaatgg ctgcccatac tgttattcgc cagccacaga tcttcagccg gataaatatc
      661 ggacatgcca ggcacgataa tacggcaagc gtagacaccc agatgctcgt aatcggcaat
      721 ataaacttct ttatcttcct ttttgaagat agccatcagc gtggcgaact cttcttccgt
      781 ggtgccggag aagctccagt caacaaacgg ataatcggca tcctgcttga acaggtccca
      841 ggagattaaa ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc
      901 ttcatcatcg aaggttggtg gagtaaacac atccagatct ttcaggccac gaccttgcag
      961 caactcggtc acggtacgtt ccagtgcaac gccaaaatca ggatgcgcac cgaatgaggc
     1021 aaagcaggta ccgttagcag gattgaacag taccacgcaa atcaccggat actggccgcc
     1081 aagcgaaccg tcataagcga agatcgggaa accttccgct tccagcgttt cgatcgcttc
     1141 aactactgct ggataacgtg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc
     1201 agcaataatg cggtttttca cgtaacgttc gaaaacttcg gacaacccct gaacgcgtgc
     1261 ttcgttacgg gtattgccag cagacatacc gttagaaacg tacaagttac caatgatatt
     1321 catcggaata taaatggtct gattgtcgga ctggcgcgta aacggtagac cgcaaatacc
     1381 acgatcttcg ttaccggatt gcaggtcaat cagcatacta ccggtcagtt cattctccgg
     1441 atcgtaaaat gcgcgcagac ggtcatcgag cagcccttct ggtacatcgt cattttcggt
     1501 cagtgggaac catttttcgt tgggataatg cacgaatggg ccgttggcga tggtttcgcc
     1561 cagccagaag tccgcaaaaa agtagttggt tgagagacgt tcgaaatatt caccgagggc
     1621 agaagccagc gccgcttttt tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc
     1681 tttgtcgcga atatgtacag accagacatt aggcacggga ttcagccagg aggcctcttc
     1741 aatctgaaaa ccgaggtctg aaagtttttg ctggaagcgt gcgatggaat cttccagagc
     1801 ggcatctttg ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc
     1861 gcgcaataat acgggtttta tctcaaaggt gctatcaccg ccgccacgct gatgaactgt
     1921 tagctatgct tttagcggat taactacgat aagagcataa aaatgaaggc gtttgatctc
     1981 caccgtatgg cattcgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt
     2041 ctttatacct ttgtactggt ctttttgttc ctcaaaatga ccggaagacg cggtgtgcgg
     2101 cagatgtcgc tgtttgaagt gttaatcatt ctgacactgg gttcagcggc gggagatgtg
     2161 gcgttttatg atgatgtgcc gatggtcccg gtacttatcg tctttattac tctggcgttg
     2221 ttataccgcc tggtaatgtg gttgatggcg cacagtgaaa aactggaaga tcttctggaa
     2281 ggcaagccgg ttgtcattat tgaagatggc gagctggcct ggtcgaaact caataactcc
     2341 aacatgacgg aatttgagtt ctttatggag ctacgattgc gcggtgtaga gcagttgggg
     2401 caggtacgtc tggcgattct cgaaaccaac gggcaaatca gtgtttattt ctttgaagat
     2461 gacaaggtga aaccgggatt acttatttta cccagtgact gtactcagcg ttacaaagtg
     2521 gtgccggagt cggcggacta tgcctgcatc cgttgcagtg aaatcattca tatgaacgcg
     2581 ggggaaaaac aattatgtcc gcgctgtgca aatccagaat tgacgaaggc aagtcgggct
     2641 aaacgggtga cctgacagcc aaaacatcgg ttttttgaaa ataatccgag agattctttt
     2701 gtgtgacgcg tgcctcattt ttgccctcaa cggttttact cattgcgatg cgtgtcactg
     2761 aatgataaaa ccgatagcca caggaataat gtattgcctg tggtcgcaat tgattgaccg
     2821 cgggttaatg gcaacgcaac gtggtgaggg gaaatggctc aaatcttcaa ttttagttct
     2881 ggtccggcaa tgctaccggc agaggtgctt aaacaggctc aacaggaact gcgcgactgg
     2941 aacggtcttg gtacgtcggt gatggaagtg agtcaccgtg gcaaagagtt cattcaggtt
     3001 gcagaggaag ccgagaagga ttttcgcgat cttcttaacg tcccctccaa ctacaaggta
     3061 ttgttctgcc atggcggtgg tcgcggtcaa tttgctgctg taccgctgaa tattcttggt
     3121 gataaaacca ctgcagatta tgttgatgcg ggttactggg cagcaagtgc tatcaaagaa
     3181 gcgaaaaaat actgcacacc aaatgtgttt gatgcgaaag tgaccgttga tggtcttcgc
     3241 gcagttaagc caatgagtga atggcaactc tctgataatg ctgcttatat gcattactgc
     3301 ccgaacgaaa ccattgacgg tatcgctatc gatgaaacac cagacttcgg taaagacgtg
     3361 gtggtcgccg ctgacttctc ttcaaccatt ctttcccgtc cgattgatgt cagccgttat
     3421 ggtgtaattt acgctggcgc gcagaaaaat atcggcccgg ctggcctgac aatcgtcatc
     3481 gttcgtgaag atttgctggg caaagcgaat atcgcctgcc catcgattct ggattactcc
     3541 atcctcaacg ataacgattc catgttcaac acgccgccga catttgcctg gtacttatct
     3601 ggcctggtct tcaaatggct gaaagcgaac ggcggtgtgg ctgcaatgga taagatcaat
     3661 cagcaaaaag cagaactgct gtatggggtg attgataaca gcgatttcta ccgcaatgat
     3721 gtggcgaaag ctaaccgttc gcggatgaac gtgccgttcc agttggcgga tagcgcgctt
     3781 gataaattgt tcctcgaaga gtctttcgct gcgggcttac atgcgctgaa aggtcaccga
     3841 gtggtcggcg gaatgcgtgc gtctatttat aacgccatgc cgctggaagg tgttaaagca
     3901 ctgacagact tcatggttga gttcgaacgt cgtcacggct aatgtcgaaa ttttgcataa
     3961 tccccacagc cagcctgtgg ggtttttatt tctgttgtag agagttgagt tcatggaatc
     4021 cctaacgtta caacccatcg ctcgtgtcga tggcactatt aatctgcccg gttccaagag
     4081 cgtttctaac cgcgctttat tgctggcggc attagcacac ggcaaaacag tattaaccaa
     4141 tctgctggat agcgatgacg tgcgccatat gctgaatgca ttaacagcgt taggggtaag
     4201 ctatacgctt tcagccgatc gtacgcgttg cgaaattatc ggtaacggtg gtccattaca
     4261 cgcagaaagt gcccgggagt tgttcctcgg taacgccgga acggcaatgc gtccgctggc
     4321 ggcagcactt tgcctgggta gcaatgatat tgtactgacc ggtgaaccgc gaatgaaaga
     4381 acgcccgatt ggtcatctgg tggatgcgct gcgccagggc ggggcgaaga tcacttacct
     4441 ggaacaagaa aattatccgc cattgcgttt acagggcggc tttactggcg gcaacgttga
     4501 tgttgatggc tccgtttcca gccagttcct caccgcactg ttaatgactg cgcctcttgc
     4561 gccggaagat acggtgattc gtattaaagg cgatctggtt tctaaacctt atatcgatat
     4621 caccctcaat ctgatgaaga cgtttggtgt tgaaattgaa aatcagcact atcaacaatt
     4681 tgtcgtaaaa ggggggcagt cttaccagtc tccgggcact tatttggttg aaggcgatgc
     4741 atcttcggct tcttatttcc tggcagcagc agcaatcaga ggcggcactg taaaagtgac
     4801 cggcattgga cgcaacagta tgcagggtga tattcgcttt gctgatgttc tggaaaaaat
     4861 gggcgcgacc atttgctggg gcgatgatta tatttcctgc acgcgtggtg aactgaacgc
     4921 cattgatatg gatatgaacc atattcccga tgcggcgatg accattgcca ccgcggcgtt
     4981 atttgcaaaa ggcaccacca cgctgcgcaa tatctataac tggcgtgtta aagagaccga
     5041 tcgcctgttt gcgatggcaa cagaactgcg taaagtcggt gcggaagtcg aagaggggca
     5101 tgatttcatt cgcattacgc caccggaaaa actgaaattt gctgagatcg ccacttataa
     5161 tgatcaccgg atggcgatgt gtttctcgct ggtcgcatta tcagatacgc ctgtgacgat
     5221 tcttgatcca aaatgtaccg ctaaaacttt tccagactat ttcgagcaat tggcgcggat
     5281 aagtcaaccc ggctgaatga ataacgggcg ataacataga caatcctttc ttttcaaaga
     5341 cgtcggcaca ttgccggcgt tttttttcgg accttgtggg gtatttttat taatggtagc
     5401 gttaattatc aatgtaactt gttgatacat aatgtttata tataattaat caacggatga
     5461 ttcacatgaa gaatactaaa ttattgctgg cgattgcgac ctctgcagcc ttactgacag
     5521 gatgtcagaa tacgcacggt attaataccg atttggctat cagctcaggt ctgaatgctt
     5581 ataaagccgc aacattaagc gatgctgatg ctaaagcaat cgccaatcag ggctgtgtcg
     5641 aaatggacag cggcaataaa gtggcaagca aatccagcaa gtacgggaaa cgtctggcaa
     5701 aaatcgccaa agcattgggt aacaatatta atggcacgcc agtcaactat aaggtttata
     5761 tgaccagcga cgtcaacgca tgggcgatgg cgaacggttg tgttcgtgtc tacagtggcc
     5821 tgatggacat gatgaacgac aacgaaattg aaggcgttct gggccatgaa ctgggccacg
     5881 tcgcgttggg tcactcgctg gctgaaatga aagcttctta tgcgatcgtg gccgcacgcg
     5941 atgccatttc agctaccagc ggtgtggctt cccagctttc ccgttcacaa ttaggtgata
     6001 tcgcagaagg cgctatcaat gctaaatact cccgtgataa agaatccgaa gcagatgatt
     6061 tctcttttga tctgttgaag aaacgtggca tcagtaccca ggggctggtt ggtagctttg
     6121 aaaaactggc cagcctggat ggcggtcgca cccagtccat gtttgattct cacccaccat
     6181 caacagagcg tgcgcaacac atccgtgatc gtatcgcctc tggtaagtaa atcattgtca
     6241 tctttcgggc tggtcttctg ccagcccgct ataattgcgc aataaatccc catctgaata
     6301 cagacaaaac tggtttttgc acacaacgtt aacgatttgt ggcgtcggcg cgtataatgc
     6361 gcgcggttat gttaacggta cgcctgtttt aaggagataa agatgacggc aattgccccg
     6421 gttattacca ttgatggccc aagcggtgca gggaaaggca ccttgtgtaa ggctatggcg
     6481 gaagcgttgc aatggcatct gctggattcg ggtgcaattt atcgcgtact ggcactagcg
     6541 gcattacatc accatgttga tgttgcatcg gaagatgcgc tggtgccgct ggcatcccat
     6601 ctggatgtac gttttgtgtc aaccaacggt aatctggaag tgatcctcga aggggaagat
     6661 gtcagcggcg aaattcgtac tcaggaggtg gcgaatgctg cttcacaagt cgcggcattc
     6721 ccacgcgttc gtgaagcatt attgcgtcgc caacgcgcgt ttcgcgaatt accaggcctg
     6781 attgccgatg ggcgcgacat gggaacggtg gtattccctg atgcgccagt gaaaattttc
     6841 cttgacgcct cctcggaaga acgtgcgcat cgccgcatgc tacagttgca ggagaagggc
     6901 tttagtgtta actttgagcg ccttttggcc gagatcaaag aacgcgacga ccgcgatcgt
     6961 aaccgagcgg tagcgccact ggttccggca gccgatgctt tagtgttgga ttccaccacc
     7021 ttaagcattg agcaagtgat tgaaaaagcg ctacaatacg cgcgccagaa attggctctc
     7081 gcataagcga ccgaatttgc agtacccccg ttgcaatgga atgacagcgg gtatgttaaa
     7141 caaccccatc cggcatggag ccaggtggac gttaaatata aacctgaaga ttaaacatga
     7201 ctgaatcttt tgctcaactc tttgaagagt ccttaaaaga aatcgaaacc cgcccgggtt
     7261 ctatcgttcg tggcgttgtt gttgctatcg acaaagacgt agtactggtt gacgctggtc
     7321 tgaaatctga gtccgccatc ccggctgagc agttcaaaaa cgcccagggc gagctggaaa
     7381 tccaggtagg tgacgaagtt gacgttgctc tggacgcagt agaagacggc ttcggtgaaa
     7441 ctctgctgtc ccgtgagaaa gctaaacgtc acgaagcctg gatcacgctg gaaaaagctt
     7501 acgaagatgc tgaaactgtt accggtgtta tcaacggcaa agttaagggc ggcttcactg
     7561 ttgagctgaa cggtattcgt gcgttcctgc caggttctct ggtagacgtt cgtccggtgc
     7621 gtgacactct gcacctggaa ggcaaagagc ttgaattcaa agtaatcaag ctggatcaga
     7681 agcgcaacaa cgttgttgtt tctcgtcgtg ccgttatcga atccgaaaac agcgcagagc
     7741 gcgatcagct gctggaaaac ctgcaggaag gcatggaagt taaaggtatc gttaagaacc
     7801 tcactgacta cggtgcattc gttgatctgg gcggcgttga cggcctgctg cacatcactg
     7861 acatggcctg gaaacgcgtt aagcatccga gcgaaatcgt caacgtgggc gacgaaatca
     7921 ctgttaaagt gctgaagttc gaccgcgaac gtacccgtgt atccctgggc ctgaaacagc
     7981 tgggcgaaga tccgtgggta gctatcgcta aacgttatcc ggaaggtacc aaactgaccg
     8041 gtcgcgtgac caacctgacc gactacggct gcttcgttga aatcgaagaa ggcgttgaag
     8101 gcctggtaca cgtttccgaa atggattgga ccaacaaaaa catccacccg tccaaagttg
     8161 ttaacgttgg cgatgtagtg gaagttatgg ttctggatat cgacgaagaa cgtcgtcgta
     8221 tctccctggg tctgaaacag tgcaaagcta acccgtggca gcagttcgcg gaaacccaca
     8281 acaagggcga ccgtgttgaa ggtaaaatca agtctatcac tgacttcggt atcttcatcg
     8341 gcctggatgg cggcatcgac ggcctggttc acctgtctga catctcctgg aacgttgcag
     8401 gcgaagaagc agttcgtgaa tacaaaaaag gcgacgaaat cgctgcagtt gttctgcagg
     8461 ttgacgcaga acgtgaacgt atctccctgg gcgttaaaca gctcgcagaa gatccgttca
     8521 acaactgggt tgctctgaac aagaaaggcg ctatcgtaac cggtaaagta actgcagttg
     8581 acgctaaagg cgcaaccgta gaactggctg acggcgttga aggttacctg cgtgcttctg
     8641 aagcatcccg tgaccgcgtt gaagacgcta ccctggttct gagcgttggc gacgaagttg
     8701 aagctaaatt caccggcgtt gatcgtaaaa accgcgcaat cagcctgtct gttcgtgcga
     8761 aagacgaagc tgacgagaaa gatgcaatcg caactgttaa caaacaggaa gatgcaaact
     8821 tctccaacaa cgcaatggct gaagctttca aagcagctaa aggcgagtaa ttctctgact
     8881 cttcgggatt tttattccga agtttgttga gtttacttga cagattgcag gtttcgtcct
     8941 gtaatcaagc actaagggcg gctacggccg cccttaatca atgcagcaac agcagccgct
     9001 taatttgcct ttaaggaacc ggaggaatca tgaccaagtc agaattgata gaaagacttg
     9061 ccacccagca atcgcacatt cccgccaaga cggttgaaga tgcagtaaaa gagatgctgg
     9121 agcatatggc ctcgactctt gcgcagggcg agcgtattga aatccgcggt ttcggcagtt
     9181 tctctttgca ctaccgcgca ccacgtaccg gacgtaatcc gaagactggc gataaagtag
     9241 aactggaagg aaaatacgtt cctcacttta aaccaggtaa agaactgcgc gatcgcgcca
     9301 atatttacgg ttaagttttt actcaaactt gaacgcgaga aaagcacctg tcgggtgctt
     9361 ttttcatttc tctaatctgg aactggaagc tgcctcgcag agttttgagc agttttcacc
     9421 ctatcgttaa attcttctga acgtgcctcg gggaacgcaa aactcccacc agacaaccgt
     9481 tcaacaaagt tgcacacttt ctataaacag ggagggtgca tgaaaataac gacagtcggc
     9541 gtatgcataa tttgcggaat ttttccgttg ctgattttgc cccaattgcc agggatagta
     9601 acccttgcgt ttctgacttt cttcgcctgt gtactggcat ttatccctgt taaaaccgtc
     9661 cgttatatcg cgctgacgtt gctgtttttc gtttggggca tattagcagc aaagcaaatt
     9721 ttgtgggcag gagaaacctt aactggcgcg acgcaggatg caattgttga gatcattgct
     9781 actgacggca tgaccactca ttacggtcaa attactcatc tacaaggtcg acgtatattc
     9841 cctgcgccag gcctcgtact gtatggcgaa tatcttccgc aagcggtttg tgccggacaa
     9901 gtatggtcaa tgaaactcaa agttcgtgca gttcatggtc aacttaatga tggcggcttt
     9961 gatagccagc gttatgccat tgcccagcat caaccgctca ccggccgttt tctgcaggca
    10021 agtgtcattg aaccgaattg tagcctgcgt gcacagtatc tggcgtcact acaaacaacg
    10081 ctgcaaccct atatgtggaa cgcggttatt cttggattag gtatggggga acggttatcc
    10141 gtccccaaag aaatcaaaaa tatcatgcgt gatactggaa cggcgcattt aatggcgata
    10201 tcaggattgc acatcgcttt tgcggcgttg ctggctgcag gactcattcg cggtgggcaa
    10261 gtttttctgc ctgggtgctg gatccactgg caaatgccat taattggtgg aatctgctgt
    10321 gctgcttttt atgcctggct tacgggaatg caacctcctg cattgcgtac cgtggtggcg
    10381 cttgccattt ggggaatgct taagttaagt ggacgacaat ggagtggctg ggatgtatgg
    10441 atatgttgtc tggcggcaat tttgctgatg gatcctgttg ccattctctc gcaaagttta
    10501 tggctctctg ccgctgcggt cgcggcactg attttttggt atcagtggtt tccctgtccg
    10561 gagtggcagc tgccgccggt attgcgcgcg gttgtttccc tcatccatct gcaactggga
    10621 atcaccctcc tgcttatgcc cgtgcaaatt gtcatatttc atggcattag tctgacctcg
    10681 tttattgcaa atctttttgc aattcccctg gtgacattta tcacggttcc gttgattctc
    10741 gccgcgatgg ttgtgcattt aagcgggccg tttattctgg aacaagggtt atggtttctt
    10801 gccgaccggt ctttggcttt acttttctgg gggttaaaga gtttgccgga agggtggatc
    10861 aacattgctg aacgttggca atggctaaca ttttccccat ggttcttact ggtggtatgg
    10921 cgattaaacg cctggcgaac gttgccagca atgtgtgtgg ctgtaggctt gctgatgtgc
    10981 ttgccgctgt ggcaaaaacc tcgacctgac gagtggcaag tgtacatgct tgatgtcggg
    11041 caagggctgg caatggtcat tgcgcgaaac ggcaaagcga ttctctatga cacgggactg
    11101 gcctggcccg aaggggatag tgggcaacaa ctgattatcc cctggctcca ctggcataat
    11161 cttgaaccgg aaggcgttat tttgagtcat gaacatctgg atcaccgggg agggctggac
    11221 tcaatattgc acacatggcc gatgttatgg atccgaagtc cgttaaactg ggaacatcat
    11281 cagccctgtg tgcgtggcga agcgtggcaa tggcaaggat tgcgtttcag cgcgcactgg
    11341 cctttacaag gtagcaacga taaaggaaat aaccattcct gtgtggttaa gattgatgac
    11401 gggacgaata gcattcttct aaccggtgat attgaagccc ccgctgaaca aaagatgcta
    11461 agccgttact ggcagcaaat gcaggcaaca ttacttcagg tacctcacca tggcagtaat
    11521 acctcatcat cgttgccatt aattcagcga gtgaatggaa aagtggcact cgcatcggca
    11581 tcgcgctata acgcatggcg actgccctca agcaaagtta aacatcgcta tcaacaacag
    11641 ggttataagt ggcttgatac tccacatcag ggtcaaataa cggtcaattt ttcagcgcaa
    11701 ggctggcgga ttagcagcct cagagagcaa attttacctc gttggtatca tcagtggttt
    11761 ggcgtgccag tggataacgg gtagaatatg cggctatttc aacaaatgct ggttttttga
    11821 atgcataacg acaaagatct ctctacgtgg cagacgttcc gccgactgtg gccaaccatt
    11881 gcgcctttta aagcgggtct gatcgtggcg ggcgtagcgt taatcctcaa cgcagccagc
    11941 gataccttca tgttatcgct ccttaagcca cttcttgatg atggctttgg taaaacagat
    12001 cgctccgtgc tgatgtggat gccgctggtg gtgatcgggc tgatgatttt acgtggtatc
    12061 accagctata tctccagcta ctgtatctcc tgggtatcag gaaaggtggt aatgactatg
    12121 cgtcgccgcc tgtttggtca catgatggga atgccggttt cattctttga caaacagtca
    12181 acgggtacgc tgttgtcacg tatcacctac gattccgaac aggtcgcttc ttcttcttcc
    12241 ggcgcactga ttactgttgt gcgtgaaggt gcgtcgatca tcggcctgtt catcatgatg
    12301 ttctattaca gttggcaact gtcgatcatt ttgattgtgc tggcaccgat tgtttcgatt
    12361 gcgattcgcg ttgtatcgaa gcgttttcgc aacatcagta aaaacatgca gaacaccatg
    12421 gggcaggtga ccaccagcgc cgaacaaatg ctgaaaggcc ataaagaagt attgattttc
    12481 ggtggtcagg aagtggaaac gaaacgcttc gataaagtca gtaaccgaat gcgtcttcag
    12541 gggatgaaaa tggtttcagc ctcttctatc tctgatccga tcattcagct gatcgcctct
    12601 ttggcgctgg cgtttgttct gtatgcagca agcttcccaa gtgtcatgga tagcctgact
    12661 gccggtacga ttaccgttgt tttctcttca atgattgcac ttatgcgtcc gctgaaatcg
    12721 ctgaccaacg ttaacgccca gttccagcgt ggtatggcgg cttgtcagac gctgtttacc
    12781 attctggaca gtgagcagga gaaagacgaa ggtaagcgcg tgatcgagcg tgcaactggc
    12841 gacgtggaat ttcgcaatgt cacctttact tatccaggac gtgacgtgcc cgcattgcgt
    12901 aacatcaacc tgaaaattcc ggcagggaag acggttgctc tggttggacg atctggttca
    12961 ggtaaatcaa ccatcgccag cctaatcaca cgtttttacg atattgatga aggcgaaatc
    13021 ctgatggatg gtcacgatct gcgcgagtat accctggcgt cgttacgtaa ccaggttgct
    13081 ctggtgtcgc agaatgtcca tctgtttaac gatacggttg ccaacaacat tgcttacgca
    13141 aggactgaac agtacagccg tgagcaaatt gaagaagctg cgcgtatggc ctacgccatg
    13201 gacttcatca ataagatgga taacggtctc gatacagtca ttggtgaaaa cggcgtgttg
    13261 ctctctggcg gtcagcgtca gcgtattgct atcgctcgag ccttgttgcg tgatagcccg
    13321 atactgattc tggacgaagc tacctcggcg ttggataccg aatccgaacg tgcgattcag
    13381 gcggcactgg atgagttgca gaaaaaccgt acctctctgg tgattgccca ccgcttgtct
    13441 accattgaaa aggcagacga aatcgtggtt gtcgaggatg gtgtcattgt ggaacgcggt
    13501 acgcataacg atttgcttga gcaccgtggc gtttacgcgc aacttcacaa aatgcagttt
    13561 ggccaatgat cgaaaaaatc tggtctggtg aatccccttt atggcggcta ttgctgccac
    13621 tctcctggtt gtatggcctg gtgagtggcg cgatccgtct ttgctataaa ctaaaactga
    13681 agcgcgcctg gcgtgccccc ataccggttg tcgtggttgg taatctcacc gcaggcggca
    13741 acggaaaaac cccggtcgtt gtctggctgg tggaacagct gcaacagcgc ggtattcgcg
    13801 tgggggttgt atcgcgggga tatggtggta aggctgaatc ttatccgctg ttattgtcgg
    13861 cagataccac cacagcacag gcgggtgatg aacctgtgtt gatttatcaa cgcactggcg
    13921 cacctgttgc ggtttctccc gtgcgttctg atgcgataaa agccattctg gcgcaacacc
    13981 ctgatgtgca gatcatcgta accgacgacg gtttacagca ttaccgtctg gcgcgtgatg
    14041 tggaaattgt cgttattgat ggtgtgcgtc gctttggcaa tggctggtgg ttgccggcgg
    14101 ggccaatgcg tgagcgagcg gggcgcttaa agtcggttga tgcggtaatc gtcaacggtg
    14161 gtgtcccccg cagcggtgaa atccccatgc atctgctgcc gggtcaggcg gtgaatttac
    14221 gtaccggtac gcgttgtgac gttgctcagc ttgaacatgt ggtggcgatg gctgggattg
    14281 gtcatccgcc gcgctttttt gccacgctga agatgtgtgg cgtacaaccg gaaaaatgtg
    14341 taccgctggc cgatcatcag tctttgaatc atgcggatgt cagcgcgttg gtaagcgccg
    14401 ggcaaacgct ggtaatgact gaaaaagatg cggtgaaatg ccgggccttt gcagaagaaa
    14461 attggtggta tttgcctgta gacgcacagc tttcaggtga tgaaccagcg aaactgcttg
    14521 cgcaactaac ctcgctggct tctggcaact agttacgccg cggcagcgtt cgattgatgg
    14581 agtcatgaat gtcgctgccg cacctctctc ttgctgatgc gcgtaatctt cacctcgccg
    14641 cgcaaggcct gttaaacaaa ccccaccgtc gagcgtcgtt ggaggatatt ccggcaacga
    14701 tctcccgcat gtccttgctg caaatcgata ccattaatat tgttgcccgt agcccatatc
    14761 tggtgctttt cagtcgtctg ggaaattatc ctgcccagtg gctggatgag tctctggcgc
    14821 gtggcgaatt aatggaatac tgggcgcatg aagcctgctt tatgccgcgt agcgattttc
    14881 gtcttattcg ccaccgcatg ctggcacctg aaaaaatggg ctggaaatac aaagacgcct
    14941 ggatgcagga acatgcggcg gaaattgcac agttaattca gcatattcat gataaggggc
    15001 cggtacgttc agccgatttc gagcattctc gtaaaggtgc aagcggctgg tgggaatgga
    15061 agccgcataa acggcatctg gaaggtttat ttactgccgg aaaggtgatg gtgattgaac
    15121 ggcgcaactt ccagcgcgtt tatgatttaa cccactgtgt catgcctgac tgggatgatg
    15181 agcgcgatct cgtttcgcaa acagaagcag aaatcatcat gctggataac agtgcgcgta
    15241 gcctgggcat attccgcgaa cagtggctgg cagattacta tcggctgaaa cgtccggcac
    15301 tggcggcttg gcgcgaagcg agggctgaac agcagcaaat cattgctgtg catgttgaaa
    15361 aattgggcaa tctttggctg catgctgatt tgctgccgct actcgagcgt gcgctcgccg
    15421 gaaagctcac tgcaacgcac agcgcggtac tttcgccttt tgatcctgtt gtctgggatc
    15481 gcaaacgcgc ggagcagttt tttgatttta gctaccggct ggagtgctat atcccggcgc
    15541 cgaaacgcca gtatggctat tttgttctgc cgttattaca tcgtgggcaa ttagttgggc
    15601 gaatggatgc caaaatgcat cgccagacag gcatccttga agttatctct ctgtggttac
    15661 aggaaggcat taaaccaacg acaacgctgc aaaaagggtt acgtcaggcg attactgatt
    15721 tcgctaactg gcagcaggca acgcgggtga cattaggacg ctgcccgcaa ggcctcttta
    15781 ctgattgccg cgccggctgg gaaatagacc ccgtcgcata aatgattatg ataagattta
    15841 tgcattcata acccgatcca tctggaggaa ctatggatca tcgtctgctt gaaatcattg
    15901 cctgcccggt ttgcaacgga aaactttggt ataaccagga aaaacaagag cttatttgca
    15961 aactggacaa cctggctttc ccgctgcgcg atggcattcc agtgttgctg gaaacggaag
    16021 cccgcgtgct gactgctgat gagagtaaat catgagtttt gtggtcatta ttcccgcgcg
    16081 ctacgcgtcc acgcgtctgc ccggtaaacc attggttgat attaacggca aacccatgat
    16141 tgttcatgtt cttgaacgcg cgcgcgaatc aggtgccgag cgcatcatcg tggcaaccga
    16201 tcatgaggat gttgcccgcg ctgttgaagc cgctggcggt gaagtatgta tgacgcgcgc
    16261 cgatcatcag tcaggaacag aacgtctggc ggaagttgtc gaaaaatgcg cattcagcga
    16321 cgacacggtg atcgttaatg tgcaggggga tgaaccgatg atccctgcga caatcattcg
    16381 tcaggttgct gataacctcg ctcagcgtca ggtgggtatg gcgactctgg cggtgccaat
    16441 ccacaatgcg gaagaagcgt ttaacccgaa tgcggtgaaa gtggttctcg acgccgaagg
    16501 gtacgcactg tacttctctc gcgccaccat tccgtgggat cgtgatcgtt ttgcaaaaga
    16561 ccttgaaacc gtaggcgata acttcctgcg tcatcttggt atttatggct accgagcagg
    16621 ctttatccgt cgttatgtta cctggcagcc aagtccgtta gaacatatcg aaatgttaga
    16681 gcagcttcgt gttctgtggt acggcgaaaa aatccacgtt gctgtcgccc aggaagttcc
    16741 tggcacaggt gtggataccc ctgaagatct caagcgcgtt cgcgccgaaa tgcgctaatt
    16801 tcacttcacg acactccagc caattttggg aggagtgtcg taccgttacg attttcctca
    16861 atttttcttt tcaacaattg atctcattca ggtgacatct tttatattgg cgctcattat
    16921 gaaagcagta gcttttatga gggtaatctg aatggaacag ctgcgtgccg aattaagcca
    16981 tttactgggc gaaaaactca gtcgtattga gtgcgtcaat gaaaaagcgg atacggcgtt
    17041 gtgggctttg tatgacagcc agggaaaccc aatgccgtta atggcaagaa gctttagtgc
    17101 gcccggaaaa gcccgacaac tggcatggaa aaccaccatg ctggcaagaa gtgggactgt
    17161 ccgtatgccg actatttatg gtgtgatgac gcatgaagaa caccccggcc ccgatgtcct
    17221 gctactggag cgaatgcgtg gtgtttcggt ggaggcacca gcccgaacac cagaacgctg
    17281 ggaacaactc aaagatcaaa tcgttgaagc cttactggcc tggcaccgtc aggacagtcg
    17341 tggttgcgtc ggcgcggtcg acaatactca ggaaaatttc tggccctcat ggtaccggca
    17401 acatgttgaa gtgctatgga ccacgctcaa tcagttcaat aacaccggtc taacgatgca
    17461 ggataagcgg atcctgtttc gcactcgcga atgtctcccg gcattatttg aaggctttaa
    17521 cgacaattgt gtactgattc acggtaactt ctgtttacgt agcatgctga aagattcgcg
    17581 cagcgatcag ttactggcga tggtcggacc gggactaatg ctttgggcac cgcgagaata
    17641 cgaactgttc cgactaatgg ataattctct ggcggaagat ttgctctgga gttatctgca
    17701 acgcgcgcca gtggcggagt cgttcatctg gcggcgttgg ttgtatgtgt tatgggatga
    17761 agttgcgcaa ctggtcaaca ccggacgatt tagtcggcgt aacttcgatc tggcatcaaa
    17821 atcactcttg ccgtggctcg cctgacgaac ctttcagcca ctgccagatc cggccaagcg
    17881 tttcatagcc aacgtggtcg ctatgcatca accaaaccgg agaagggatt gcccgttccc
    17941 acggattaag cggcgagtcg atggccaact gattcgccgg ggctggtagc ggatttaacc
    18001 cttcctgctg aaaaaagatc atcgcccgcg gcagatgtga tgcggaggtc accagcagga
    18061 aaggtgcgtc accaatcgcc tgtttcactg ctgcagcttc ttcttcggta tcttttggca
    18121 aatccagggt gataatttgc tcgcgcggca cgcccagcga ttgcgcaact ctggcaccta
    18181 cttcggctgt gctcaccgta ttggttttag ctacgcctcc cgtaaagatc agttttgatc
    18241 ccggattttc gcgccataag cgaatacctt cattcaggcg tggcagactg ttattgatta
    18301 aattagagct cggcgcccac tgcgggttcc aggtataacc acctccgagc accacgatat
    18361 agtccacttt ttgcgaatta ttccatgtcg gataagtgct ttcgatggga cgcaacaggc
    18421 ggtcggctat cggttgcaaa ctcaataaca agagcgccag ccacccaatg ctaataaaaa
    18481 tcttcccggt tttttgaaaa cggctaaacc acagcagcgc caggccagcg ccaataatca
    18541 acaacatcag cggaagtggc agcaacatat tgccaatcac ttttttcagt gtaaaaagca
    18601 tcctttctgg ttcctttttt gaccatatag caagggatcg ccagcgatat tacaccagac
    18661 gggttcattc tcgccgtggc tgtgacaaaa tagcgtcttt atcagaaaac gtcttagcca
    18721 accggtggag aaagcaatgc aggatcgcaa ttttgatgat attgcggaaa agttttcccg
    18781 taacatttac ggcaccacca aagggcagct tcgacaggct attctgtggc aggatctcga
    18841 tcgcgtgctg gcggaaatgg gcccgcaaaa actgcgtgtg ctggatgctg gcggtggaga
    18901 agggcagacc gcaatcaaaa tggccgagcg cgggcatcag gtcattttat gcgatctttc
    18961 tgcgcagatg atcgaccgcg caaaacaggc ggcagaagca aaaggtgtga gcgacaacat
    19021 gcaatttata cattgcgccg ctcaggatgt tgcttcgcat ttggaaacgc ccgttgatct
    19081 gatattgttc catgcggtgc tcgagtgggt ggccgatccc cgcagcgtat tacagaccct
    19141 gtggtcagtg ttgcgtccag gcggcgtgtt gtcgttaatg ttctacaatg cgcatggttt
    19201 gttgatgcat aacatggtcg ccgggaattt tgattacgtg caggcgggaa tgccgaaaaa
    19261 gaaaaaacgg acgctttcgc cagattatcc acgcgacccg gcgcaggttt atctgtggct
    19321 ggaagaagct ggttggcaaa ttctgggtaa gacaggcgtt cgcgtgtttc atgattatct
    19381 gcgcgagaag caccagcagc gcgactgcta tgaagcatta cttgaattag aaacgcgtta
    19441 ttgccgtcag gaaccgtata ttaccctggg gcgttatatt catgtcaccg cgcgcaaacc
    19501 gcagagcaag gataaagtat gagtgaattt tcccagacag tccccgaact ggttgcctgg
    19561 gccagaaaaa atgacttctc catctcgctg ccggtagacc gactctcttt tctgctggcg
    19621 gttgccacgc tgaacggcga gcgtctggat ggtgagatga gtgaaggcga gctggtggat
    19681 gcattccgcc atgtgagtga tgcgtttgag caaaccagcg aaaccatcgg cgtgcgcgcc
    19741 aacaacgcga tcaacgacat ggtgcgtcaa cgtctgctga accgctttac cagcgagcag
    19801 gcggaaggga acgcaattta ccgtctgacg ccgctcggca tcggcatcac tgactattac
    19861 attcgtcagc gcgagttttc tacgctgcgc ctttctatgc aattgtcgat tgtggcgggt
    19921 gagctcaaac gcgcagcaga tgccgccgaa gagggcggtg atgaatttca ctggcaccgt
    19981 aatgtttatg cgccactgaa atattcggta gcagaaattt tcgacagtat cgacctgacg
    20041 caacgtctga tggatgaaca gcagcagcag gtgaaggatg atatcgccca gttgttgaac
    20101 aaagactggc gggcggcgat ttccagctgt gaattgttgc tttcagaaac ttccggaacg
    20161 ctgcgtgaat tgcaggatac gctggaagcg gcaggcgata aattgcaggc caacctgtta
    20221 cgcattcagg atgcgacgat gacccatgac gacctgcatt ttgtcgatcg tctggtgttc
    20281 gatcttcaga gcaaactcga ccgtattatc agttggggcc agcaatccat cgacttgtgg
    20341 attggctacg accgccacgt acacaaattt attcgtactg cgatcgatat ggataaaaac
    20401 cgcgtctttg ctcagcggtt acgtcagtcg gtacaaacct attttgatga gccgtgggcg
    20461 ctaacttatg ccaatgccga tcgtctgctg gatatgcgtg acgaagagat ggtactgcgc
    20521 gatgaagaag tgactgggga acttcctgag gatctggagt acgaagagtt taacgagatc
    20581 cgcgaacagc tggcggcgat catcgaagaa caacttgccg tgtacaaaac cagacaagtg
    20641 ccgttggatc ttggtctggt ggtacgcgaa tatctgtcac agtatccgcg tgcacgtcac
    20701 tttgacgttg cgcgcattgt tattgatcag gcggtacgtc ttggcgtagc gcaagcagat
    20761 ttcaccggac tgccagcgaa atggcagccg attaatgatt acggagccaa ggtacaggcg
    20821 catgtcatcg acaaatattg aacaagtgat gccggttaag ctggcgcagg cgctggcgaa
    20881 tccgttattt ccggcgctgg atagcgcctt acgttcaggc cgtcatattg gcctcgacga
    20941 actggataat catgcattcc tgatggattt tcaggaatat ctggaagagt tttacgcgcg
    21001 ttataacgtt gagcttattc gtgcaccgga agggttcttc tatctacgcc cacgttccac
    21061 cacgctgatc cctcgttccg tcttgtcgga actggatatg atggtcggga aaatcctctg
    21121 ttatctctat ctcagcccgg aacggctggc gaatgagggg atttttactc aacaggaact
    21181 gtacgacgaa ctgctcaccc tggccgatga agcgaaactg ctgaaactgg tgaacaaccg
    21241 ttcaaccggt tcagacgttg accgtcagaa gttgcaggaa aaagtacgtt cttcgctcaa
    21301 ccgtctgcgt cgtttaggca tggtgtggtt tatgggccac gacagcagca agttccgcat
    21361 taccgaatcg gtgttccgct tcggagccga tgtgcgtgct ggcgacgatc cccgtgaagc
    21421 acagcgtcgc ctgattcgtg atggtgaagc aatgccgatt gaaaaccatc tgcaactcaa
    21481 cgatgaaacc gaagagagtc agccagatag cggagaggaa gaataatgat tgaacgcggt
    21541 aaatttcgct cactgacgct gattaactgg aacggctttt ttgcccgaac ttttgacctt
    21601 gacgagctgg tcacgacgct ttctggcggt aacggggcgg gtaaatccac caccatggcg
    21661 gcgttcgtta cggcgcttat ccccgacctg accctgctgc atttccgtaa cactacggaa
    21721 gccggggcca ccagcggttc gcgcgataaa ggtctgcacg gtaagctgaa agcgggtgtc
    21781 tgttattcga tgctcgacac cattaactcg cgccaccagc gtgtagtggt cggtgtgcgt
    21841 ctgcaacagg ttgccggacg cgatcgtaaa gtggatatca aaccatttgc cattcagggc
    21901 ctgccgatgt cggtgcagcc gacacaactg gtgaccgaaa ctctgaacga acgccaggcg
    21961 cgcgtgctgc cgcttaacga gctgaaagac aagctcgagg cgatggaagg cgtgcagttt
    22021 aaacagttca actccattac cgattaccac tcgctgatgt tcgatctggg catcatcgcg
    22081 cgtcgtctgc gctcagcgtc tgaccgtagt aaattctatc gtctgatcga agcctcgctg
    22141 tatggcggga tctccagcgc cattactcgt tctctgcgcg actacctgtt gccggaaaac
    22201 agtggggtgc ggaaagcgtt ccaggacatg gaagcggcgc tgcgtgaaaa ccgcatgacg
    22261 ctggaagcga ttcgtgtcac ccagtcggac cgtgacctgt ttaagcatct gatcagcgaa
    22321 gccaccaact acgtggcggc ggactacatg cgtcacgcca acgagcgccg tgttcatctc
    22381 gataaggccc tggagtttcg tcgcgagcta catacttcgc gtcagcaact ggcggctgag
    22441 cagtacaaac acgtcgacat ggcgcgagag ctggctgagc acaacggtgc cgaaggcgat
    22501 ctggaagcgg attatcaggc ggccagcgat cacctgaacc tggtgcaaac cgcactgcgt
    22561 cagcaggaga agatcgaacg ctacgaagcg gatctcgatg agctgcagat ccgtctggaa
    22621 gagcaaaacg aagtggtggc agaagccatc gaacgccagg aagagaacga ggctcgtgcg
    22681 gaagctgccg aactggaagt ggacgagctg aaaagccagc ttgctgacta ccagcaggcg
    22741 ctggacgtcc agcaaacgcg cgcgatccag tataaccagg cgattgctgc gcttaatcgt
    22801 gccaaagaac tgtgccatct gccggactta accgccgaca gcgctgccga atggctggaa
    22861 accttccagg cgaaagagct ggaagcgacc gaaaaaatgc tctctcttga gcagaaaatg
    22921 agcatggcgc aaaccgcgca cagccagttt gagcaggctt atcagctggt ggtggcaatc
    22981 aacggcccgc tggcgcgtaa cgaggcgtgg gatgtcgctc gcgaactatt gcgcgaaggg
    23041 gtcgatcagc gtcacctggc agagcaggtt cagccgttgc ggatgcgctt aagcgaactg
    23101 gaacagcgtc tgcgtgagca gcaagaagct gagcgtctgc tggcagattt ctgcaaacgt
    23161 cagggcaaga attttgatat tgatgaactg gaagctctgc atcaggagct ggaagcacgc
    23221 attgcctctc tttccgatag cgtctctaac gcccgagaag agcggatggc actgcgccag
    23281 gagcaggaac agctgcagtc tcgcattcag agtttgatgc agcgtgcgcc ggtttggctg
    23341 gcagcgcaaa acagtctcaa ccagttgagc gaacagtgcg gcgaagagtt cacctccagc
    23401 caggatgtca ccgaatttct gcaacagttg ctggagcgtg agcgcgaggc aattgttgaa
    23461 cgcgatgaag taggcgcgcg caaaaacgcc gtcgatgaag agatcgaacg tttaagccag
    23521 ccaggtggtt ctgaagatca gcgtctgaac gcgctggcgg agcgttttgg tggtgtgctg
    23581 ctgtcagaaa tttatgacga cgttagcctg gaagatgcgc cgtacttctc agcgctgtat
    23641 ggcccgtcac gccacgccat cgtggtgcca gatctgtcac aggtaaccga acacctggaa
    23701 ggcttgaccg attgcccgga agatctctat ctgatcgaag gggatccgca gtcattcgat
    23761 gacagcgtgt tcagcgttga tgagctggaa aaagcggtgg tggtgaaaat cgccgatcgt
    23821 cagtggcgtt attcacgttt cccggaagtg ccgctgtttg gtcgtgctgc gcgtgaaagc
    23881 cgtattgaaa gcctccatgc cgagcgggaa gtgctttccg aacgcttcgc cacgctctcc
    23941 tttgatgtac agaaaaccca gcgtctgcat caggcgttca gccgctttat cggcagtcat
    24001 ctggcggtgg cgtttgagtc tgacccggaa gcagaaatcc gtcaactgaa cagccgtcgc
    24061 gtcgaactgg aacgggcgtt gagtaatcat gaaaatgata accagcagca gcgtattcag
    24121 tttgagcagg cgaaagaggg cgttacggcg ctgaaccgca ttctgccgcg tctcaacctg
    24181 ttggctgatg acagcctggc ggatcgcgtc gatgaaatcc gcgaacgtct ggatgaagcc
    24241 caggaagccg cgcgttttgt tcagcagttt ggcaatcaac tggcgaaact ggaaccgatc
    24301 gtttcggtat tgcagagcga cccggaacag ttcgaacagt taaaagaaga ttacgcgtac
    24361 tctcagcaga tgcagcgcga tgcccgtcag caggcgtttg ccctgacgga agtggtgcag
    24421 cgtcgtgcgc actttagcta ctctgactcg gcagaaatgc ttagcggtaa cagcgatctc
    24481 aacgaaaaac tgcgtgaacg tctggaacag gcggaagcgg agcgtacccg cgctcgtgaa
    24541 gcgttgcgcg gacacgcagc gcagttgaat cagtacaacc aggtgctggc ttcgctgaaa
    24601 agttcgtatg acaccaaaaa agagctactt aacgatctgc aacgtgaatt gcaggatatc
    24661 ggcgtgcgtg ctgatagcgg ggcagaagag cgggcacgta ttcgccgtga cgagttgcac
    24721 gcgcaactga gcaataaccg ttcacgacgc aatcaactgg aaaaagcgct tacattctgc
    24781 gaagcggaga tggacaacct gacccgtaaa ctgcgcaagc tggagcggga ttactttgag
    24841 atgcgcgagc aggtagtgac cgccaaagcg ggctggtgtg cggtgatgcg catggtgaaa
    24901 gataatggcg ttgagcgtcg cttacaccgt cgtgagctgg cttatctctc cgctgatgat
    24961 ttgcgttcca tgtcggataa ggcgttaggt gcgctgcgtc tggcggtggc agataacgaa
    25021 catctgcgcg acgtgctgcg gatgtcggaa gatccaaaac gtccggaacg taaaattcag
    25081 ttcttcgtgg cggtttatca gcatctgcgt gaacgtattc gtcaggatat tattcgtacc
    25141 gatgatccgg tggaagctat cgaacagatg gagatcgagc ttagccgtct gaccgaagaa
    25201 ttaacctccc gtgaacagaa actggcgatc agttcccgca gcgtggcgaa catcattcgc
    25261 aaaaccattc agcgcgagca gaaccgtatc cgtatgctca accaggggtt gcagaacgta
    25321 tcgtttggcc aggtgaacag cgtacgtctg aacgtgaacg tacgtgaaac gcacgccatg
    25381 ctgctggatg tgctctctga acagcacgag cagcatcagg atctgtttaa cagcaaccgc
    25441 ctgaccttct cagaagcgct ggcgaaactg tatcaacgtc ttaacccgca gattgatatg
    25501 gggcagcgca cgccgcagac catcggtgaa gaactgctgg attaccgcaa ctatctggaa
    25561 atggaagttg aggttaaccg tggttccgat ggctggttgc gtgcagagtc tggtgcattg
    25621 tcgaccggtg aggcgattgg taccggtatg tcgattctgg tgatcgtggt acaaagctgg
    25681 gaagatgaat ctcgccgcct gcgcggtaaa gatatctctc cttgccgcct gctgttcctc
    25741 gatgaagcag cgcgactgga tgcccgttct atcgccacgc tgtttgaatt gtgtgaacgt
    25801 ttgcaaatgc agctcatcat cgcagcgccg gaaaatatca gcccggagaa aggcaccacc
    25861 tataaactgg tgcgtaaagt cttccagaat accgaacacg ttcacgtcgt cggcctgcga
    25921 ggatttgcgc cgcaactccc tgaaacgctt ccaggaagtg acgaagcgcc ttctcaggcg
    25981 agttaaaatt aaggcggcag taatgccgcc ttttcttttt ccgaaaactc cgtttctgca
    26041 ctaaaaaagt ggcacattac ggcgcgcttt tctttaaact tctttacatt aggttatgta
    26101 aaaacgtatc ggcgtttata tactgaagat aagcctgatg agtaacaggc ttgctcgtca
    26161 tactttcgtg agtattggcg ttgtacaggc aagtcgtaaa ataacagcct ggctattcag
    26221 agtatgataa aaacaggggg caagggatgt tgcttaatat gatgtgtggt cgtcggctgt
    26281 cggcaatcag tttgtgcctg gccgtaacat tcgctccact gttcaatgcg caggccgatg
    26341 agcctgaagt aatccctggc gacagcccgg tggctgtcag tgaacagggc gaggcactgc
    26401 cgcaggcgca agccacggca ataatggcgg ggatccagcc attgcctgaa ggtgtggctg
    26461 aaaaagcccg cacgcaaatc gaatctcaat tacccgcagg ttataagccg gtttatctta
    26521 accagcttca attgttgtat gccgcacgcg atatgcaacc catgtgggaa aaccgtgatg
    26581 ctgttaaagc cttccagcaa cagctggcag aggtggcgat tgccggtttc cagccgcagt
    26641 ttaataaatg ggtagagtta ctgaccgatc ctggtgttaa cgggatggca cgcgacgtgg
    26701 tgctctctga tgcgatgatg ggctatctcc atttcattgc aaatattccg gtcaaaggca
    26761 ctcgctggct atatagcagt aaaccttatg cgctttcaac gccgccgctt tcggtgatta
    26821 accaatggca gctggcgctg gataaaggtc aattgcctac gtttgttgca ggactggcac
    26881 cgcagcatcc gcaatatgcg gcgatgcatg aatcgttact ggccttactc agtgacacca
    26941 aaccgtggcc ccaactgacc ggcaaagcaa cgttgcgccc aggacagtgg agtaacgacg
    27001 taccggcgtt gcgcgaaata ttgcaacgta caggcatgtt ggacggggga ccgaaaatta
    27061 cgctacctgg cgatgacacg ccaactgacg cggtagtcag cccatccgct gttactattg
    27121 aaacagcaga aactaagccg atggacaagc aaactacgtc tcgtagtaaa cctgcgcctg
    27181 tcgttcgtgc cgcctacgat aatgaactgg tggaagccgt taaacgtttt caggcatggc
    27241 aaggattggg ggcagatggt gctattggcc cggcaacgcg tgactggtta aacgtaacgc
    27301 ccgcccagcg tgctggcgtg ttggcactca acatccagcg attgcgcttg ctgccaacag
    27361 agctttctac cgggatcatg gttaacattc cggcctattc gctggtctac tatcagaacg
    27421 gcaatcaggt gctggattcg cgagtcattg tcggtcgccc cgatcgcaaa acgccaatga
    27481 tgagcagtgc ccttaacaac gtagtggtaa acccgccgtg gaacgtccca ccaactctgg
    27541 cacgcaaaga tattctgccg aaagtgcgca acgatccggg atatctcgaa agccatggct
    27601 atacggtgat gcgcggctgg aacaacagag aagcgattga cccatggcag gttgactggt
    27661 ctacaatcac ggcctcgaat ttaccgttcc gcttccagca ggctccaggc ccacggaact
    27721 cgctggggcg ctataaattc aatatgccga gttcagaggc catttatttg catgacacgc
    27781 cgaaccacaa tctgttcaag cgtgatacac gcgcattgag ctcaggctgt gtacgagtga
    27841 ataaagcttc cgatctggcg aatatgctgt tgcaggatgc aggctggaat gacaaacgta
    27901 tttctgatgc gctgaagcag ggcgatacgc gttacgtcaa tattcggcag tcgattccgg
    27961 tgaatctcta ctatctgacg gcctttgttg gtgcagatgg tcgtacccag tatcgtacag
    28021 atatttacaa ttatgatctg cctgcgcgat ccagctcgca aattgtatcg aaagcggaac
    28081 aattaatcag gtaaatgaag cagttcgggg aaatcagttg tcgtaataat tagcatgatc
    28141 gggggcgatt ctctgcagcc cccgtcactg ctggggttga gtcatcttga cgtctgcttt
    28201 acgggcggtt aaggtgcctc ttgtgcgcca gaagtgcata taaacgataa cattgacctg
    28261 tagacttgat tatcatggac aaattcgacg ctaatcgccg caaactgctg gcgcttggtg
    28321 gcgttgcact cggtgccgcc atcctgccga ccccggcgtt tgcaacactc tctaccccac
    28381 gcccgcgcat tttgacactc aataatcttc ataccggaga gtcaatcaaa gcggagtttt
    28441 tcgatggcag aggctatatt caggaagaat tggcaaaact taaccatttt ttccgcgatt
    28501 accgcgcgaa caaaataaag tccatcgacc caggattatt cgaccagttg tatcgcctgc
    28561 aagggttgtt aggcactcgc aaaccggtgc aactcatttc cggttatcgt tccattgata
    28621 ccaacaatga actacgcgcc cgcagccgtg gagtagcgaa gaaaagctat cacactaaag
    28681 gccaggcgat ggatttccac attgaaggta tcgcgttaag caatattcgc aaagccgcgt
    28741 tatctatgcg cgcaggtggt gtaggatatt acccacgtag taactttgtg catattgata
    28801 ccgggccagc acggcactgg tagtaatcgc ttaacgaaac aggggcagta tgaactatcg
    28861 tattattccg gtcaccgcat tctcccagaa ctgttcattg atctggtgtg aacaaacccg
    28921 tctggccgca ctggtcgatc ctggcggcga tgcggaaaaa atcaaacagg aagttgatgc
    28981 cagcggcctg acactgatgc agatcctgct gactcatggt catctggacc acgttggcgc
    29041 agcggcggaa ctggcgcaac attacggcgt gccggttttt ggcccggaaa aagaagatga
    29101 gttctggctg caaggcttgc ctgcgcaaag tcgtatgttt ggtctggaag agtgccagcc
    29161 gctgacgcca gatcgttggc tgaacgaagg cgataccatc agcataggga atgtgacttt
    29221 acaggtgtta cattgccctg ggcatacgcc gggtcatgtc gtgtttttag atgatcgggc
    29281 aaagctgctg atttctggcg acgttatttt caaaggtgga gtagggcgca gtgacttccc
    29341 gcgtggcgat cataatcaac tgatttcttc aatcaaagat aaattgctgc cactggggga
    29401 tgacgtgaca tttattccgg gtcacggacc attatccaca cttggttatg agcgcctgca
    29461 taatcccttc ctgcaagacg aaatgcccgt ctggtaaaaa ggcgcataaa aaagcccgct
    29521 ttcaagcggg cttcatcggt tttaacgctt acagcactgc cacaatcgct tcgcacagcg
    29581 gcgccatgtt atctggtgtc atcccggcca cgtttacgcg accagaagca acagcatata
    29641 cgccaaactc ttcgcgcaga cgcagcactt gttcttttgt caggccactg aaggagaaca
    29701 tgccgttctg tttgatgata aagctgaagt cgcgatttgc gcctttttcc tgcagcgtat
    29761 tgacgaacaa ctgacgcata cgctgaatac gctggcgcat atcagtcagt tcttgttccc
    29821 aaatcgcacg taacgcatcg ttgctcagga tggtggcaac aacagaagcg ccgtgtgctg
    29881 gtgggttaga gtagttagca cgaatcgccg ctttcatttg gctgaatgcg cgttcaacag
    29941 tttcactgtc agcagcaacc agagtacaag cgccaacacg ctcgttgtac aggccaaagt
    30001 ttttagagta ggaactggca acaatcagct ccttatgcag agccgcgaaa gcgcgcagtc
    30061 cttcagcatc ttcttccaga ccacgggcaa aaccctggta agcgaagtca aacagcggta
    30121 accagccttt ctcaacggag agttgtgcca gtgtctgcca ttgttccagc gtagggtcga
    30181 tgccggttgg gttatggcag cagccatgga acagcactac gtcgccagcc tgggcttcgt
    30241 tcaggctgtt aatcagtgca tcgaagtcaa gagtgtgatt ttccgcatca taataagcgt
    30301 attcacgaac ttccagacct gcagagttaa agacgctctt atggttcggc cagcttgggt
    30361 tgctcaccca cacacgctta acgctggtat tttttgccag gaaatcggca gccacgcgta
    30421 gtgcgccagt gccacccgga gtctgtgccg tgcgagcacg tttgtcattg atcagggcgc
    30481 tacctttacc aaacagcagt tcctgagtgc agcgaccaaa ttcagggatg ccgtcaatgc
    30541 cgaggtaatt tttggtggtt tcattttcga gcagatactg ttcagccttt ttcacgctgg
    30601 tcagtaccgg ggttttaccc gtctcatctt tatagacacc aatcccgagg ttaattttgc
    30661 cgggacgttc atcggcacga aacagatcgg ccaggcccag aatcgggtcg gcaggagcgg
    30721 cggtaatgtt ctcaaacatg acgaggttcc attatggtta cagaagggaa gtccgctatc
    30781 agggtaacgg gagatttaca aaattccaac tattactgat gaaaacgcag gctgtttttg
    30841 caagacatga gattgctctg gaaggcataa aaaaaaacag gaccaaagtc ctgttttttc
    30901 ggcatttaac aaagaggtgt gctattagaa ctggtaaacg atacccacag caacggtgtc
    30961 gtcagaacct acgcccagtt tgttgtcaga atcgatctgg ttgatgatgt agtcaacata
    31021 ggtggacatg tttttgttga aatagtaggt tgcgcccact tcaaagtagt tcaccagatc
    31081 aacatcaccg ataccttcta cgtctttcgc tttagatttg gtgtaagcga tggacggacg
    31141 cagaccgaaa tcaaactggt attgcgcaac taacagaacg tcttgcgttt tgttggcgaa
    31201 gccgctggtg tttgtaaatt tattagtgat cggcgtagcg ttacgggttt caccgtagtt
    31261 cgctgccagg tagatgttgt tcgcgtcata cttcagacca gtagcccact gttcagcttt
    31321 tttgcctttg ccaagggaag actcttcctg caggttggta cggtcagcgg caccataagc
    31381 accaacgata ccaaagcctt cgtattcgta gctgatagaa ccgccaacac cgtcgccgtt
    31441 agagcggcgt gcagtgtcac gctcgttttt acccaggtac tgaacagcga agttcagacc
    31501 atcaaccaga ccaaagaagt tggagttacg ataggtagca acgccgccaa cacgaccaac
    31561 gaagaaatca tcgctgtatg cagtatcacc accaaattct ggcagcatat cggtgtaacc
    31621 cagtgcatca taaaccacac cgtagttacg gccgtaatcg aaagaaccaa cgtcagcgta
    31681 tttaagaccc gcgaatgcca gacgcgtttt gttaccagtt tgagcgtcag cgccctcaga
    31741 gttgttaccc tggaagttat attcccactg accataaccg gtcagatcgg aattgatttg
    31801 agtttcccct ttaaaaccaa gacgggcata ggtcatgtcg ccattgccac cgtaactgtt
    31861 ttcaccgtta cccttggaga aataatgcag accgacagct ttaccgtaca gatctacttt
    31921 gttgccatct ttgttataga tttctgcagc gtttgcagta cctgctacta acagagcagg
    31981 gacgatcact gccagaatat tgcgcttcat cattatttat taccctcatg gtttttttta
    32041 tgacacctgc cactgccgtc aataagttct gtcaataaaa atttacggaa ctattgatga
    32101 gagtttggtg tctttatgtg tctgcaggca tctttccatt caaactaacg tttcgctacc
    32161 gtgaaagtgc tacaaagata aagatttagt ttcaaaaaga aaaaatatgt gacaaaaagt
    32221 aaaaattaag gaactttgtg aacaccgtca tat
//
LOCUS       DAZGJT010000048        31233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZGJT010000048 DAZGJT010000000
VERSION     DAZGJT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.048"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..297
                     /gene="ymgC"
                     /locus_tag="ACHKFK_002460"
     CDS             49..297
                     /gene="ymgC"
                     /locus_tag="ACHKFK_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO9052780.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIAQALRLRLK
                     DETSVILSDLYLQILQHIEMHKTTLTDIIINDRESVLS"
     gene            595..1478
                     /locus_tag="ACHKFK_002461"
                     /pseudo
     CDS             595..1478
                     /locus_tag="ACHKFK_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic diguanylate phosphodiesterase"
     gene            1610..1828
                     /gene="ymgF"
                     /locus_tag="ACHKFK_002462"
     CDS             1610..1828
                     /gene="ymgF"
                     /locus_tag="ACHKFK_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO9052781.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITPLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQH"
     gene            2226..3744
                     /locus_tag="ACHKFK_002463"
                     /pseudo
     CDS             2226..3744
                     /locus_tag="ACHKFK_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768545.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3803..4138)
                     /gene="ymgD"
                     /locus_tag="ACHKFK_002464"
     CDS             complement(3803..4138)
                     /gene="ymgD"
                     /locus_tag="ACHKFK_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO9052782.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4142..4486)
                     /gene="ymgG"
                     /locus_tag="ACHKFK_002465"
     CDS             complement(4142..4486)
                     /gene="ymgG"
                     /locus_tag="ACHKFK_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO9052783.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(4488..4661)
                     /locus_tag="ACHKFK_002466"
     CDS             complement(4488..4661)
                     /locus_tag="ACHKFK_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052784.1"
                     /translation="MSYLSFEIILIPVKNIIPIITVTLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDN"
     gene            complement(4812..5078)
                     /gene="minE"
                     /locus_tag="ACHKFK_002467"
     CDS             complement(4812..5078)
                     /gene="minE"
                     /locus_tag="ACHKFK_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO9052785.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5082..5894)
                     /gene="minD"
                     /locus_tag="ACHKFK_002468"
     CDS             complement(5082..5894)
                     /gene="minD"
                     /locus_tag="ACHKFK_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO9052786.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5918..6613)
                     /gene="minC"
                     /locus_tag="ACHKFK_002469"
     CDS             complement(5918..6613)
                     /gene="minC"
                     /locus_tag="ACHKFK_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO9052787.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEAPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7133..7501
                     /gene="ycgJ"
                     /locus_tag="ACHKFK_002470"
     CDS             7133..7501
                     /gene="ycgJ"
                     /locus_tag="ACHKFK_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO9052788.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGNDAGGLQSPACGVVCDPYIC
                     VSSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFSNGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(7604..8005)
                     /gene="pliG"
                     /locus_tag="ACHKFK_002471"
     CDS             complement(7604..8005)
                     /gene="pliG"
                     /locus_tag="ACHKFK_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO9052789.1"
                     /translation="MKIKGISKAVLLLALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8247..8540
                     /locus_tag="ACHKFK_002472"
     CDS             8247..8540
                     /locus_tag="ACHKFK_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO9052790.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKQALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDINK"
     gene            8612..9271
                     /gene="ycgM"
                     /locus_tag="ACHKFK_002473"
     CDS             8612..9271
                     /gene="ycgM"
                     /locus_tag="ACHKFK_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO9052791.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            9363..9809
                     /gene="ycgN"
                     /locus_tag="ACHKFK_002474"
     CDS             9363..9809
                     /gene="ycgN"
                     /locus_tag="ACHKFK_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO9052792.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKGL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(10016..10339)
                     /gene="hlyE"
                     /locus_tag="ACHKFK_002475"
                     /pseudo
     CDS             complement(10016..10339)
                     /gene="hlyE"
                     /locus_tag="ACHKFK_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            10602..11021
                     /gene="umuD"
                     /locus_tag="ACHKFK_002476"
     CDS             10602..11021
                     /gene="umuD"
                     /locus_tag="ACHKFK_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO9052793.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLTPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            11021..12289
                     /gene="umuC"
                     /locus_tag="ACHKFK_002477"
     CDS             11021..12289
                     /gene="umuC"
                     /locus_tag="ACHKFK_002477"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO9052794.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVHNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADSDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFAFNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPCYTTRSSDLLRVK"
     gene            complement(12334..12864)
                     /gene="dsbB"
                     /locus_tag="ACHKFK_002478"
     CDS             complement(12334..12864)
                     /gene="dsbB"
                     /locus_tag="ACHKFK_002478"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO9052795.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMARFPEWLPLDKWVPQVFVASGDCAERQWEFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(13010..14551)
                     /gene="nhaB"
                     /locus_tag="ACHKFK_002479"
     CDS             complement(13010..14551)
                     /gene="nhaB"
                     /locus_tag="ACHKFK_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO9052796.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRLQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            14773..15492
                     /gene="fadR"
                     /locus_tag="ACHKFK_002480"
     CDS             14773..15492
                     /gene="fadR"
                     /locus_tag="ACHKFK_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO9052797.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(15655..17187)
                     /gene="ycgB"
                     /locus_tag="ACHKFK_002481"
     CDS             complement(15655..17187)
                     /gene="ycgB"
                     /locus_tag="ACHKFK_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO9052798.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKHPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYNGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            17517..18815
                     /gene="dadA"
                     /locus_tag="ACHKFK_002482"
     CDS             17517..18815
                     /gene="dadA"
                     /locus_tag="ACHKFK_002482"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO9052799.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQTGVKF
                     HFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            18825..19895
                     /gene="dadX"
                     /locus_tag="ACHKFK_002483"
     CDS             18825..19895
                     /gene="dadX"
                     /locus_tag="ACHKFK_002483"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO9052800.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPYARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHQEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(19952..21688)
                     /gene="cvrA"
                     /locus_tag="ACHKFK_002484"
     CDS             complement(19952..21688)
                     /gene="cvrA"
                     /locus_tag="ACHKFK_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO9052801.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIVMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(21783..22697)
                     /gene="ldcA"
                     /locus_tag="ACHKFK_002485"
     CDS             complement(21783..22697)
                     /gene="ldcA"
                     /locus_tag="ACHKFK_002485"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO9052802.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLSVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTQEGTQLTISGHPVLKM"
     gene            22797..23408
                     /gene="emtA"
                     /locus_tag="ACHKFK_002486"
     CDS             22797..23408
                     /gene="emtA"
                     /locus_tag="ACHKFK_002486"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO9052803.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQIKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(23410..24144)
                     /gene="ycgR"
                     /locus_tag="ACHKFK_002487"
     CDS             complement(23410..24144)
                     /gene="ycgR"
                     /locus_tag="ACHKFK_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO9052804.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLNKAAIPLRISWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRITAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELHEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREARKKADNVRD"
     gene            24345..24599
                     /gene="ymgE"
                     /locus_tag="ACHKFK_002488"
     CDS             24345..24599
                     /gene="ymgE"
                     /locus_tag="ACHKFK_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9052805.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(24649..26619)
                     /locus_tag="ACHKFK_002489"
     CDS             complement(24649..26619)
                     /locus_tag="ACHKFK_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9052806.1"
                     /translation="MRLKKRYLCTVLSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLRAVEVSKGYSSLLQGPNQMGGAINITTQKPTKPLEANLGYRQG
                     WSRSQDNAYDMHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMINSSA
                     DDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNSGQKSRYWQWPEYDKESFYY
                     QGTTQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQLAAD
                     VRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVAGISY
                     DWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRFPTLK
                     ERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTHDWGFEVSVYYNRVSDAILSH
                     NIDADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKITDLPT
                     QTMTAWMTLKPWEPLSVTLSEEARSSSYSNNDGSQKAAGFAVTHIRADYTLGHGFSVN
                     ASVNNLFDTKYAYSEGFIEEGRNFWAGIEYTF"
     gene            complement(26645..27499)
                     /gene="modD"
                     /locus_tag="ACHKFK_002490"
     CDS             complement(26645..27499)
                     /gene="modD"
                     /locus_tag="ACHKFK_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HFO9052807.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQHGYIEFFLRQGG
                     CVSGISVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQDWSGAINQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPAASI"
     gene            complement(27496..28308)
                     /locus_tag="ACHKFK_002491"
     CDS             complement(27496..28308)
                     /locus_tag="ACHKFK_002491"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9052808.1"
                     /translation="MLIDDIDFADLYLQQLRLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLTSKIDLQGAQTLFDMGCGPGTVSLALADKLTTIYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRQAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVVELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYRWYQQQDARALAPASRDWALIWWDSVPQETLR"
     gene            complement(28318..29076)
                     /locus_tag="ACHKFK_002492"
     CDS             complement(28318..29076)
                     /locus_tag="ACHKFK_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9052809.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLPVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHPLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTKKLAALYRVSADQIHHHLSAIKH"
     gene            complement(29073..30053)
                     /locus_tag="ACHKFK_002493"
     CDS             complement(29073..30053)
                     /locus_tag="ACHKFK_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9052810.1"
                     /translation="MRTVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENVPV
                     QEQIVFWQIRVPRILAALFLGAALAGAGTTYQGMFRNPLVSPDILGVSAGAGLGACAA
                     ILWGLSIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWVGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLFLLLRGGRQ"
     gene            complement(30053..31075)
                     /locus_tag="ACHKFK_002494"
     CDS             complement(30053..31075)
                     /locus_tag="ACHKFK_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HFO9052811.1"
                     /translation="MSLTRRRFTQILASTLFLHHLPSFAQSVKFWASRTLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQFPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETWISQARQVSEQTQIPWLLLNGKLEQSAEQLTTLGKTLG
                     EEHRAAEQANLASRFVGEAQAFATSPAANLSFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDIQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         7960 a         7465 c         7866 g         7942 t
ORIGIN      
        1 actatacaca gcataatttt attggatcac tattcctgcg aggccgacat gaataattca
       61 atcccagaga gatttatttt tcaatgtgct ttgtttaaaa atctcgagag ggaggtgttc
      121 atgacacatg gatatgttga tagtcatatt attgctcagg ctttacgctt gcgtttaaaa
      181 gatgagacca gtgttattct ttctgatctc tatctgcaaa tcttgcagca cattgaaatg
      241 cataagacta cactaacgga tatcattatt aatgacaggg aatccgtgct ctcttaaaac
      301 ttgagtaagg tttttgaata aacatcagct gtatcactat gctgatgcaa agtgagcatt
      361 tcaggcgtta tgctttctta atatatccgc aatatcagag ggcaaacagg aatggagagc
      421 gctcgttttc cattcttgac gcctgatatc ccgcctaatt tgtttaattc tgcctacatt
      481 gtgctaaaaa attaataggc tagatgaagt tggagaaaat tttatcttcg gcgtggatat
      541 ttgtgatgct ctctaccatg ctctctgtag accccagcgc gagcgaatga acgtctcttt
      601 agcttctaca gacttatcga taaaggcttt gggattttga tatttattct gttaatcgcc
      661 tgcgccgctg ccttcctgct tgataggtat ttcaataaaa gcgcaacgcc tgaagagatc
      721 ctgcgacggg ctataaataa tggggagatc gtcccttttt accaacctgt ggtaaatggt
      781 cgggaaggga cattgcgggg agttgaggtg ttagcccgct ggaaacaacc tcacggtgga
      841 tatatatcat tattccactt gctgaaaaat gcggattaat cgttccgctt acgcaaagcc
      901 tgatgaatca ggttgccaga cagatgaacg ctatcgcgag taaactgccg gaaggttttc
      961 atattgggat taattttagc gcctcgcata ttatttcgcc gacgtttgtc gacgagtgct
     1021 taaattaccg tgacagtttt acccgccgcg atttaaacct tgttctggaa gtcaccgagc
     1081 gtgagccatt aaatgttgat gaaagtctgg ttcagcggtt gaacattctg catgaaaatg
     1141 gttttgtcat cgcgctggat gatttcggta ctggttactc agggctttct tatctgcatg
     1201 acctgcatat tgattatatc aaaattgatc acagtttcgt tggccgcgtc aacgcagacc
     1261 cagcatcaac ccgaattctg gattgtgtat tggatctggc gcgtaaactt tcgatcagta
     1321 tcgtcgctga aggtgtcgaa acgaaagaac aatttgacta tctgaaccaa aataatatca
     1381 catttcagca gggttattat ttctataaac ctgttacata tatcgacatg gtcaaaatta
     1441 tcctttctaa accgaaggtg aaggttgtgg ttgagtgaaa attgatcagc aaggccatgg
     1501 tgcggtgtaa ttatagacag ctaattagct cgttgcctct tgttactatt gttcattctt
     1561 ttgtttgcta taattgtgtg aaagttttga ctggattgcc attagtagca tgaacaatag
     1621 taataatctg gattatttca ctctctatat catcttttcc attgcattta tgctgatcac
     1681 tcccctggtc atccttattg caaaacccag caccgggctg ggagaagtgc ttgtgacgat
     1741 aaatttgctt aatgccctgg tttggctggc gatcaatctg gttaatcgat taagggaaag
     1801 actcgtcagc cacagggatc agcactaatc tttcagtttc tcactgccag tatgcggctg
     1861 aatgggttgc tggcagtgaa cccctggatc attgaaggaa aggaattatt gcacaaatag
     1921 ttgtcaaccc tggtgttatc acagttgttt tatatatcag tgaaataatt tccatggcaa
     1981 ctgttgataa ttttgatgtc gaagagttat ttgttaaaca aaatagttac cccaaagtga
     2041 tcaatgtcat gaaaataagc taaaaatgat aatgctggct tatttatcat ttaaatattg
     2101 tcgctgttta tctatttaaa tttcttgtgc ttttgttttt atctttgcct tccgttttgg
     2161 taatctgaac tcatagggta tttgaccagg aatgtttgtt aatccaatga atggatggtt
     2221 aatctatgaa actgaaaaaa ctcccctggt tcagtttagg acttattgct ctggcggtgg
     2281 gtaatgcata tgcaacacaa ttgttggata attatagtat aatttcctat ataaccgatg
     2341 aagaatcacc gattgaaatc aaaaataata cttctacaag taatggggag tatctaatca
     2401 cttaagacga aagccatgcc gtgaaagtgg atgacggtgt aactggatat atacataatg
     2461 ccagtgtgat gactagtggt gatggatctt atggtatttc tgttgatagc caaaacaaag
     2521 tattatatat aagcgatagc aatattaaga cgctctgaaa gcgtatctga caaagaaaat
     2581 ggagggataa cagccagcgc agtagtcagt gaatttggtg gcaccatcgt tatgaatggt
     2641 gataattcag tcgagacgag ggggcatatt cagcgggact tttaagtcag gttaatgatt
     2701 ctgtgactgt ggaaaataac accagtcttg aaaccacaga taaaacgaac attgttacct
     2761 atgggaaaaa tgcagtagga gttcttgcat gttcaagtcc tggagagtct cgaacatgtg
     2821 tcgatgctgt agatgatgaa gtttgtgatt ctaacagtta cgaagttatt agccgtgctg
     2881 atttaaaaat gaatggtggt tccataacaa ctaatggtat taatagctat ggtgcttatg
     2941 ctaatgggaa aaaagcatat attaatttag attatgtggt acttgaaact gtggctgatg
     3001 gaagttatgc agttgctatt cgacaaggta acattgatat aaaaaagttc tattacaaca
     3061 aatggcacta aagcccccat tgcaaaaata tacaatggta gagagttatt tttttccaat
     3121 gtcacttcag aatcaacaga aacgcctacc cccgtcctgc gacccgaagc tggcagttac
     3181 gtcgccaacc tggcagccgc taacactctt tttgttatgc gtctgaacga ttgtgcaggt
     3241 gaaacgcgct atatcgatcc tgtaactgaa caggtgcgtt caagccaact ttggttacgt
     3301 caaattggcg ggcataatgc ctggcgtgac agcaacggac agttgagaac gacctcggca
     3361 caggccatct ggatgggcgt cgatcataat gcgcacacgg aagccaatgg ctcacgtatt
     3421 gagaatgacg caaataacaa cattcagatc cgacttggtt tccgcacctt tattcgtact
     3481 caggagaaaa acagcggtcc gcacggtgac ggctttgaac cttttgttga aatgaacggg
     3541 atccataaca gtaaagattt tgctgtctca ataaacggtg tgaaagttga acaagacggg
     3601 gcgcgtaatc tgggggaaat gaaacatggc gtaaatggca atctgaatcc ggcggccagc
     3661 gtctggggta atgtgggcgt gcagctgggt gataatggct acaatgacac cgcaatgatg
     3721 gtgggcctga aatataagtt ctgatcccgc ccgttagcta aaaaaccgcg tcgaattcat
     3781 cgacgcggta acatatgaaa tattattttt tgccgatagc acgcatggtg tcatcaattg
     3841 ccgtgatcaa cagcatttgc ggatctttag cgcaaacctg attcagtttt tctaccactt
     3901 tggcgctcag ttccggagat tgctcaattt ttaaatcacg gctggcaacg ctggcattac
     3961 ccattaccgc aacaatttct gcaacctgtg cgctgtcagt ttttgccatt tcgttggctt
     4021 ctgcacaagt aatataggtt tctgatggca aaccgttctt aatattgtag tcctgcgccc
     4081 aggtcattgg tgcgaaaaca aacagacccg ccagtaaagc aaattttttc atcatcattc
     4141 cttatttcat tttacccaga attgcaccac ccgtaccgcc aatcacggca cctttaatcg
     4201 ccccttcgag gccattgccg gtcagaacgc cagtgacagc accaacggcg gcacccactt
     4261 ttgcaccttt acgcgcattt ttaccgtcgc ggcctttttc tgttactgca ccaacaccag
     4321 cgccaacagc tgcacctttc agtacgccat taacaccatt gccagtaagt aaaccaacgc
     4381 ctgcgcctag caatgcacct ttcgtggtgc gattcatatc cgccatcgct ggcgtggagc
     4441 agaacaatgc tgagataagc ccgaaggcaa gtattttttt cttcaactta gttgtccggt
     4501 attaagtaag ttgcacacac aataatttcg tcttcaatta agatctgctt aactaaagaa
     4561 cgctcgctat tattcagata attcaaaatg agcgtgactg tgatgatagg aattatgttt
     4621 tttacgggaa tgagaataat ctcaaatgat aaataactca ttgattgaca atatttttat
     4681 tcaagaagtg tcattgactg ttaacgcaat gttgtaaagg taagataatc tgatttatca
     4741 atattattgt gcacagaaac tcaaggcaga gatgactctg ccttgaagat aaatgcgctt
     4801 ttacaggggg cttatttcag ctcttctgct tccggtaagg tcacgttcag ctcaagaata
     4861 gaaatatcgc catctttttg ctcaagctgt acggttacca tctcaggatc aatttgtaca
     4921 tatttacaaa tgacctcaag aatatcttta cgcaactgcg gcagataatg cggttctgca
     4981 tcgctgcgac ggcgttcagc aacaataatc tgcaaccgtt cttttgcaat gttggctgtg
     5041 tttttcttcc gcgagagaaa gaaatcgagt aatgccataa cttatcctcc gaacaagcgt
     5101 ttgaggaagc ctttcttctc ttcttcaatg aagcggaaag gacgttcttc tcccaacaga
     5161 cgttctacgg tatctgcgta ggctttaccc gcatcggcgt taatgtcgag aatgaccggt
     5221 tcaccctggt tagaggcgcg caatactgat tgatcctctg ggatcacgcc gacgagtttg
     5281 atgcgcagga tctccagcac atcttccatg ctcagcatgt cacctctgct tacgcggcct
     5341 gggttatagc gcgttaacag taggtgctct ttaatcggct cttcgccatt ttctgcgcgg
     5401 cgtgatttcg acgccagaat gcctaaaata cggtcagagt cgcgtactga ggagacttcc
     5461 gggttggtgg taataatggc ttcgtctgca aaatagagtg ccattaacgc accggtttca
     5521 atccctgccg gggagtcaca aacgataaat tcaaaatcca tcgctttcag atcatcaaga
     5581 actttggcga ccccttcacg ggtgagggca tctttatcgc gagtttgcga tgccggaaga
     5641 atatagagat tttcagtacg tttatcttta attaacgcct ggtttagcgt tgcatcgccc
     5701 tgaatgacgt tgacgaaatc gtaaacgacc cggcgttcac aacccataat caggtcgaga
     5761 ttacgcaggc cgatatcaaa atctatcacg acagttttct ttcccttctg ggccaaacca
     5821 gtggcgatgg ccgcgctgga ggttgtctta ccaacccccc ctttgcccga agtaacaaca
     5881 ataatgcgtg ccatagaaat tccttgttaa aaagggatca atttaacggt tgaacggtca
     5941 aagcgttttc gactaactgc agtcgtgccg ctttgccata aaattctgct gggatttgat
     6001 cactcagcca gtattcacct gcgatggaca ccagttccgc catcaggttc gtacaaaata
     6061 tttgcgtttc ccggtcacca cttgcccctg ccagcgcacg accgcgcatc atgccataga
     6121 catgaatgtt accatcggca atcaattcgg cccctgcgct aacgtggctt gtaacaatca
     6181 gatcacattg tggagcataa atacgctgac cggaacgcac tggggtatca attaaacgcg
     6241 tttttgtgac cggcgttgta ttttgcgctg gagcctgcgg tgcgggagct ggacgcggcg
     6301 ctttttcctt tccttccgtc aggataggca gccccatctt ttcaatttcg gctttaagtt
     6361 gcgcatcttt gcagccactt acgccaataa cccgcaaacc ggttgccgaa accgccttat
     6421 gcatcgctga ccagtttacc ggggcttcca gtgcactgac gttgagtaca acgggggcat
     6481 gttttaaaaa tgcgggggcc tgagcgattt tgtcttccag cgcctgatgg ataaccttag
     6541 gttctgcctc atgcagatga accacagata aagtgaagct actgccttta agctcgattg
     6601 gcgtgtttga catcctggcc ttactcaatt agctattaat catcgccagc gcgcgatgat
     6661 gttccgaaga ctataaggca tgttatagtc tggattatat tgaggcaagt caccctccca
     6721 tttattcaga gtaaaagtct attctgtgat aaatggcgct gattcatagc ttaaaaaata
     6781 cccttgtcaa tcaacccatt gccttcgtac ttttgattgt tcttatttac gcttcctttt
     6841 tccgcaccct aatcgtgaca gcatcttcag acctgtttaa atatccagtg ttgaggcgag
     6901 tgtatttttt caccacgcca ggctttctta actttccatt ttttgcagca tcattaaaga
     6961 acagataccc gttatcttaa tatccgccac agaattgctc tatcatagaa accgcagaag
     7021 acgtctgata cttctgtcag gtcatcgtaa ctcgtccagg tcagcggttt tgattacgga
     7081 ttgatgatga aatgagcata agtgttgtac ggttggatta tcaggatgtg ctatgaatat
     7141 gatgcgtatt ttttatatcg gattgtcagg tgtggggatg atgttctcat caatggcgtc
     7201 tggaaatgac gctggcgggc ttcaatctcc tgcgtgtggc gttgtttgcg acccgtatat
     7261 ttgtgtgagt tcagatggca tttccccaga gttaacaagg aaatatctcg gcgaaaaagc
     7321 cgctgaaaac ttacaatcat tacaaggcta cgatcccagc gaatttacat tctctaacgg
     7381 tgtattttgc gatgttaaag aaaaattatg tcgtgatgat cgttattttg gtgtggatgg
     7441 taagaggagt ggaaaaatca atcaaaccac cacaaagatg ttatttatgt gtcgtgagtg
     7501 atgtcttgtt tacattatgt tctattcgtc attaacctct taagcgacag agaggagtgg
     7561 ggcgttaacg tttatgacgg ggagagtccc cgtcggttga tatttactta atttgaatat
     7621 cgacgttata ttttttagtt ttgtttttac gggcgtcgtt tcgggtttgc agcactcgca
     7681 gctcatattt gccagaagca gggagtgagt attgtccgtg actatcgagt tctggggaat
     7741 atctggataa atcaacagaa tcatcgattc ctggcccgaa gaggtaagta tcagcgcctt
     7801 cattagagat actcacgtgc actttctgac cttttttggc atagaaggta tatgtatcgt
     7861 aatcgtatcc ctttatttcg ccggagtact gagcgctgct atggcctttt ctgaactcaa
     7921 cattgacatt cttacccgca gcaaagctcg tggaggttag cagtgcaagc aacaatacag
     7981 ccttactgat gcccttaatt ttcattggta gtctcctgtt cagaatgagt acgatgaact
     8041 gttataatat aacaatcctt cacgggagaa ttccagtggc tttcctgtgt tcggttaaat
     8101 cccggcgcgg catttttgcc ggggggatta cttataaagg gggcatggac tgaacaaacc
     8161 gtagaaatct acagctggct ggcttgtggc gcgggtttca ttgatgttag cttatgccaa
     8221 aacctggcat cttaaagagt aaaagtatgt tttgtgtgat ttatcgaagc agcaagcgtg
     8281 accagaccta tttatatgtc gaaaaaaaag acgatttttc gcgtgttcct gaggaactga
     8341 tgaaaggttt tggtcagcct cagttagcga tgattcttcc gctggatggg cggaagaaac
     8401 tggttaatgc cgatattgaa aaggttaaac aggcattaac cgagcaaggt tactatttgc
     8461 agttaccgcc accacccgaa gatttgctga agcaacatct ttccgtcatg gggcaaaaaa
     8521 cagacgacat taacaaataa ccgatatccg gcggtggcat tatctttgtc ggcgcgggtt
     8581 ttcatatcca cgataaggtg aggggaacgt tatgtatcaa catcacaact ggcaaggtgc
     8641 gctgctggat tatccggtga gtaaagtagt ctgtgttggc agtaactatg ccaaacatat
     8701 taaagagatg ggcagcgcag tacccgaaga gccagtgctg tttattaaac cagaaacggc
     8761 actgtgcgat ctgcggcagc cattggcgat cccatccgat ttcggttcag ttcatcatga
     8821 agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag gctacggaag agcatgtccg
     8881 caaagccatt gccggttacg gcgtggcgct cgatctgaca ttgcgtgatg ttcagggaaa
     8941 aatgaagaaa gccgggcagc cgtgggaaaa ggccaaagcg tttgataact cttgtccgct
     9001 ttccgggttt attcccgcgg cggagtttac cggcgacccg caaaatacaa cgctgggcct
     9061 gagcgtaaac ggcgagcaac gccagcaagg tacgactgcg gacatgatcc ataaaatcgt
     9121 tccgctgatc gcttatatga gcaagttttt taccctcaag gccggtgacg ttgtgctgac
     9181 aggcacgcct gatggtgtcg gcccgttgca aagcggtgat gagctgacag tcactttcga
     9241 tgggcattct ttgacaactc gcgttttgta atactttttg ccgcctgaaa gcggcggcaa
     9301 aacttgcatc gctgtgccag actggttata aggtgcgttt taacgtaatg gcggaacacc
     9361 tgatgagcga tgtacctttc tggcaaagta aaaccctgga cgaaatgagc gatgcggaat
     9421 gggagtcgtt gtgtgatggt tgcggtcagt gttgcctgca taaactgatg gatgaagaca
     9481 ccgacgaaat ctacttcact aacgtcgcct gtcgccagct caatattaaa acctgtcaat
     9541 gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg cattaaatta acccgtgaaa
     9601 atctgccaac attcgaatgg ctgccaatga cctgcgctta tcgtttgctg gcggaaggta
     9661 aaggtttacc tgcgtggcat ccgctactta ctggttcgaa agcggcaatg catggtgaac
     9721 gtatctctgt gcgtcatatc gcagtaaaag aatcagaagt gattgactgg caggatcata
     9781 tcttaaataa acctgactgg gcgcagtgat aaatttaata gatgttgatg ggggctttct
     9841 taattcacgt ctggatgctc gccatttcgt agccctgttt ttgaaagcgc tctgtcataa
     9901 tgagagttac tcgatacctt attatcaaca ggtaactctc aatctttaaa aaacgtctgt
     9961 atttttaaat gtgataaact ttataccttg agtacataga gagaataatc gcttatcaga
    10021 cttcagatac ctcaaagagt gtcttcttac cgtgtctttt ctgatactca ttacaggtgt
    10081 taattatttt tttggccgct tcttttagta aagaaagcat taaatcatca taatcaacgt
    10141 agaatctggt tgtttcagtt tccgttttta tctccccgat ggcggctatt tcggtagtta
    10201 atttcaattt ggcggcatcg atatctttat tcgcttgttt aaccgtgtta gacagggtgg
    10261 taaagaaact ctgcgcagat tttaacttgt tcttcaattc tggaatcagt ttcccttcaa
    10321 ctacgcccgc agcaatagaa cacagtttct tgatgccgtc atccagtacc ttaatgagaa
    10381 tgcctttctg ggcgaatgct tcgattctat tttaaaccag gtgatatttt tcctttctgc
    10441 tggatggacg ctgtcgccag aaggccgcgt gagcacagga taagagaacg aaaaatcagc
    10501 agtctatgca gcgacaaata ttgatagcct gaatcagtat tgatctgctg acaagaacag
    10561 actgctgtat ataaaaacag tataacttca ggcagattat tatgttgttt atcaagcctg
    10621 cggatctccg cgaaattgtg acttttccgc tatttagcga tcttgttcag tgtggctttc
    10681 cttcaccggc cgcagattac gttgaacagc gcatcgatct gaatcaactg ttgatccagc
    10741 atcccagcgc gacttatttc gtcaaagcaa gtggtgattc tatgattgat ggtgggatta
    10801 gtgacggtga tttactgatt gtcgatagcg ctattaccgc cagccatggt gatattgtca
    10861 tcgctgctgt tgacggtgag tttacggtga aaaaattgca actacgcccg acggtacagc
    10921 ttactcctat gaacagtgcg tactcgccca ttaccatcag tagcgaagat acgctggatg
    10981 tctttggtgt ggtgatccac gtcgttaagg cgatgcgctg atgtttgccc tctgtgatgt
    11041 aaacgcgttt tatgccagct gtgagacggt gtttcgccct gatttatggg gtaaaccggt
    11101 ggttgtgcta tcgaataatg acggttgcgt tatcgcccga aacgctgagg caaaggcgct
    11161 gggcgttaaa atgggcgatc cctggttcaa acaaaaagat ctgtttcgtc gctgtggcgt
    11221 ggtttgcttt agcagcaatt atgagcttta cgcagacatg agtaatcggg tgatgtcgac
    11281 gctggaagaa ctatcgcccc gcgtcgagat ttacagtatt gatgaggcat tctgcgatct
    11341 gacaggtgtg cataattgtc gcgatctgac tgattttggc agagaaattc gcgcaacggt
    11401 gctacaacgt acccatctta ctgttggtgt ggggatcgcc cagaccaaaa cgctggctaa
    11461 gcttgccaat catgcggcaa aaaaatggca gcggcagacg ggcggggtgg tggatttatc
    11521 aaatctggaa cgccagcgta aattaatgtc tgctcttccc gtggatgaag tctgggggat
    11581 tggacggcgg atcagcaaaa aactggacgc gatggggatc aaaaccgttc tcgatttggc
    11641 ggattcagat atccggttta tccgtaaaca ttttaatgtc gtgctcgaaa gaacggtgcg
    11701 tgaactgcgc ggtgaaccct gtttgcaact ggaagagttt gcaccgacga agcaggaaat
    11761 tatctgttcc cgctcgtttg gtgaacgcat cacggattat ccgtcgatgc ggcaggccat
    11821 ttgtagttac gctgcccggg cggcggaaaa acttcgcagc gagcatcaat attgtcggtt
    11881 tatctctacg tttattaaga cgtcaccatt tgcgttcaat gaaccttatt acgggaatag
    11941 cgcatcggta aaactgctga cgcccactca ggacagcagg gatatcatta acgccgctac
    12001 gcgatctctg gatgccatct ggcaagcggg ccatcgctac caaaaagcgg gcgtgatgct
    12061 gggggatttc ttcagtcagg gagtcgcgca gctcaattta ttcgatgaca acgcaccgcg
    12121 ccccgggagt gagcaattga tggcggtaat ggatacgctg aatgctaaag agggcagagg
    12181 aacactctat tttgccgggc aggggatcca gcaacaatgg cagatgaagc gtgccatgct
    12241 ttcaccatgt tatacaacgc gaagttctga tttactgcgg gtcaaataaa catagcggca
    12301 ggaaaaagcg ctcccgcagg agcgccgaat ggattagcga ccgaacaggt cacgtttttt
    12361 cgctttaaac ggctgggaaa tcaccaccag caccgcgaca atcaggtaag cgataaaaat
    12421 accgagcagc cactgcggca tttccagacc taaaaattcc cactgacgct cggcgcaatc
    12481 gccagaggcg acaaacactt gcggcaccca tttatccagc ggcagccatt ccgggaaacg
    12541 agccataaaa tcacaggtcg caaacggcga aggatagagc tgaagcatgg tgtgctcgta
    12601 agttaactgc acaccgcgga acgcactata caaccagata accatcgcta cataacgcag
    12661 cggagttttc ggggcgatcg cgccaatcag cgcagcaccc agaacgccga ataacgcgca
    12721 gcgttcataa atacagagca cgcaaggttt cagtaacatc acatgctgga accacagcgc
    12781 cgtcagttcc agtgccagag cagtaaacgc catcaacagc catgcgcccc ggccttgtga
    12841 acattggttc aaaaatcgca acataatcat ttccctgcaa tatgcataga gtgcgcagtt
    12901 taaaccaatt cattcgctgc gccaccagga ggcagcaaaa ataggacgca attggacaat
    12961 tatcgggcga aaaggcaagc cgggcattta tatgcccggt aagttgttat caaagcgttg
    13021 ctatccagcc catttgcata aaccattcgg ttacaggagc aagcgtaaac tcgacgcaga
    13081 gcagcccgac gagtgtcagg acgagggtgt aaggcagggc catccacacc atgcggccat
    13141 aagagagacg aatcaatggc gcgagtgcag aggtcagcag gaacaggaac gcagcctgac
    13201 cgttcggcgt agcgacggag ggcagattgg taccggtatt aatggcgacc gccagcagct
    13261 catattgctt caacgtgata gcgccacttt ccattgccgc tttcgcttcg ttgatataaa
    13321 tcgtccccac gaagacgtta tccgaaatgg atgacagcag accgttgaaa atatagaaca
    13381 acgacagctg agcatgttca gatgcctgca acacaaactg aataatcggc gaaaacagtt
    13441 gttggtcgat aatcaccgcg actactgaga aaaacaccgt caacagtgcg gtgaatggca
    13501 gagattcggt gaaggcttta ccgatagcat gttcatcggt aacaccggtc aatgatgtcg
    13561 ccagaataat gaccgacaaa ccaatcaaac cgacttctgc caaatgtagt gccagcgcgg
    13621 tgaccagcca gacgccgata atcgcctgca caatcagacg aattttgtcc tgacgtgtac
    13681 gctggagacg gctttgatcg tcaaactgtt gcagcacttc gcggactttc tccggcagcg
    13741 tttcaccgta gccaaaccaa cgcagcttct ctaccagcag gcaggttaac aggccacaaa
    13801 tcagaaccgg gacggtcacc ggcgacatgc gcaggaagaa atcgccaaaa tgccagccag
    13861 ccgctttagc gatgatcagg ttttgtggtt cgcccaccat ggtcatcacg ccgcctaatg
    13921 cggtgccgac accggcatgc atcatcagac tacgcagaaa gccacggaac tgttccagaa
    13981 ccactttgta atgcttgtcg atatgactat cgtcttgcag gtcggtatct tcagtacggg
    14041 aagaggctac gcggtgataa ataccataaa aaccgactgc aacgctgatc accaccgcca
    14101 cgacggttaa ggcatcgagg aacgcggaga ggaacgccgc cgccacgcaa aaagagagcg
    14161 acagcagcat tttggagcga atacttaaca gcaaacgggt aaatatgaac agcaacagct
    14221 gtttcataaa atagataccc gccaccataa acatcagtaa cagcaagact tcaagatttg
    14281 ccgccacctc ttcacggacg tgttccgcgc tggtcatgcc gatgaatact gcttcgatag
    14341 ccaacaaacc accggggagc agcgggtagc atttcagggc catagccaga gtgaaaataa
    14401 attccgctac cagcaaccag ccagcgacga aagggctgat gaggaaaatt aacggattta
    14461 cgattaagaa aatgatgagg gcgagtttgt accagtcggg ggactggccc aaaaaattgc
    14521 gccatagtgc gcggccccag gagatctcca tgatggtttc ccttacctta gaaataatca
    14581 atgatgtttt tatgtttaaa cgcaaagctt aacggtcaga caggagtgag gcaagtcttg
    14641 atagtcaagg ggaaagagat gcagaaaatg aagccttgat cccttttttc ttctttttgt
    14701 cttctatcag cgtagttagc cctctggtat gatgagtcca actttgtttt gctgtgttat
    14761 ggaaatctca ctatggtcat taaggcgcaa agcccggcgg gtttcgcgga agagtacatt
    14821 attgaaagta tctggaataa ccgcttccct cccgggacta ttttgcccgc agaacgtgaa
    14881 ctttcagaat taattggcgt aacgcgtact acgttacgtg aagtgttaca acgtctggca
    14941 cgagatggct ggttgaccat tcaacacggc aagccgacga aggtgaataa tttctgggaa
    15001 acctccggtt taaatatcct tgaaacactg gcgcgactgg accacgaaag tgtgccgcag
    15061 cttattgata atttgctgtc ggtgcgtacc aatatttcaa ctatttttat tcgcaccgcg
    15121 tttcgtcaac atcccgataa agcgcaggaa gtgctggcaa ccgctaatga agtggccgat
    15181 cacgccgacg cctttgccga gctggattac aacattttcc gcggcctggc gtttgcttcc
    15241 ggcaacccga tttacggtct gatccttaac gggatgaaag ggctgtatac gcgcattggt
    15301 cgtcactatt tcgccaatcc ggaagcgcgc agtctggcgc tgggcttcta tcacaaactg
    15361 tcggcgttgt gcagtgaagg cgcacacgat caggtatacg aaacagtgcg tcgctatggg
    15421 catgagagtg gcgagatttg gcaccggatg cagaaaaatc tgccgggtga tttagccatt
    15481 cagggacgat aatccttcca gacggatgac aaacgtataa ttgcctgatg cgctacgctt
    15541 atcaggccta cgtgtttttt tgcaatgtat tgaatttgcg atactttgta ggccggataa
    15601 ggcatttacg ccgcatccgg caaaaatgag tcctcctcaa acgaggagga ctatttacag
    15661 atttcccatt ctcggcgggc aacgttccag caactcgatg ctgccgtctt cgttttgctg
    15721 ttccagcatc acatcaaatc cccacaggcg atgcacatgc ttcagcactt ctttgcgccc
    15781 ccgatccagc ggtgcgcgat tatgtggaat gtaacgcagc gtcagcgaac ggtcgccgcg
    15841 caaatccacg ttccagatct gaatattcgg ctccagatta cttaagttat attgcgacga
    15901 taaccggtta cggatttccc gataaccttc ttcattatga atagcggaaa tctccagata
    15961 attatgccga tcatcatcca gcacggtgaa gaagcggaaa tcacgcatca ctttcggtga
    16021 caggaactgg ctgataaaac tctcatcttt gaaatcacgc atcgcgaaat gcagcgtttc
    16081 cagccagtcg gaaccggcga tatccgggaa ccagtatttg tcttcttccg ttggtgactg
    16141 acaaatccgt ttaatatcct ggaacatggc gaatccgaga gcatatgggt taatgccgtt
    16201 gtaccacggg ctgttgtaag gcggctggaa gaccacattg gtgtggctgt gcaaaaactc
    16261 cagcataaaa cgttccgtta ctttcccttc atcatacaga tggttaagga tggtgtagtg
    16321 ccagaaggtc gcccagcctt cgttcatcac ctgagtctgt ttttgcggat aaaaatactg
    16381 gctcacctta cgcacaatac gcaggatttc acgctgccat gattccagca gtggcgcatt
    16441 tttctccata aaatagagca ggttttcttg tggttcggac ggatagcggc gcgcttcagc
    16501 aaccgttttc tcttcctcgc gcttcggcag ggtacgccag agcatattga cctgactttg
    16561 cagatactct tcgcgactct tctgccgggc tttctcttct tgcagcgaga ttttttgcgg
    16621 gtgtttgtac cggtccacgc cgtagttcat cagcgcgtgg cacgagtcca gaagccgttc
    16681 tacttcatca acgccataac gctcttcgca ctcggtaata tatttacggg caaaaatcag
    16741 ataatcgaca atcgaactgg cgtcggtcca gctacggaat aagtaattgt ttttgaagaa
    16801 agagttatgc ccatagcagg catgagccat caccagcgct tgcatggtaa tggtgttctc
    16861 ttccatcagg taagcgatac acgggttaga gttaatgacg atttcatagg ccagtccttg
    16921 ctgaccgtgc ttatacagcc gttcagtctc gataaacttt ttaccgaatg accagtgcgg
    16981 atagttaatt ggcatgccga cgctggagta ggcatccatc atctgttctg aggttatcac
    17041 ttcaatctgg tgcgggtagg tatccagccg gtagagtttc gccacccggt ctatctctgc
    17101 cagataaaca tccagcaggt cgaacgtcca gtcgggtcca tcgctcaaac gtgtggtgtc
    17161 cttattcata gaatcgatcg tcgccatacg cgcacctcat tgttgtcggc gctctctgtg
    17221 tggagcacct catttcaagc atagaacacc tgttaaaaac cgcgtcgccg gagaattttt
    17281 ttctttgcga tttcttatta tcagagtgcc actaatccgc ttctgaacgg aattttatgc
    17341 tggataaaaa gggcgttcag caggagatac taaagacgcc atattgccgc agagtcaggg
    17401 agatgtgagc cagctcacca taaaaaagcc gtatgttgaa taatattttc aactgagtta
    17461 tcaagatgta attagattat tattctttta ctgtatctac cgttatcgga gtggctatgc
    17521 gagttgtcat actgggaagt ggtgtggtag gcgttgccag cgcctggtac ttaaatcagg
    17581 caggacatga ggtcaccgtc attgatcggg agccgggggc cgccctggaa accagtgccg
    17641 cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg ggcggcacca ggtgtgcctt
    17701 taaaagcgat taaatggatg ttccagcgcc atgcgccgct ggcggttcgt ctcgacggta
    17761 cgcagttcca gttgaaatgg atgtggcaaa tgttacgtaa ctgcgacacc agccactaca
    17821 tggaaaacaa agggcggatg gtacgtctgg cggaatacag ccgtgattgc ctgaaagcat
    17881 tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg tgggacgctg caactgttcc
    17941 gtaccgaaca acagtatgaa aacgcgaccc gcgatattgc cgtgctggaa gatgctggcg
    18001 taccgtatca gctgctggaa tccagccgcc tggcggaagt ggagcccgcg ttggcagaag
    18061 tggcgcacaa actgacgggc ggcctgcaac tgcccaatga tgaaaccgga gactgccagc
    18121 tgtttaccca gaatctggcg cggatggcgg agcagacggg ggttaaattc cactttaata
    18181 cgcccgttga ccaactgctt tgcgacggcg agcaaatcta cggcgtgaag tgtggtgatg
    18241 aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc ttactcgacg gcgatgctca
    18301 aaggcattgt tgatattccg gtgtatccgc tgaaaggcta ctcgctgact attccgattg
    18361 cgcaggaaga tggtgcgccg gtgtctacta tccttgatga aacctacaaa atcgccatta
    18421 cccgtttcga taaccgcatt cgtgttggcg gaatggcgga aattgttggc tttaacaccg
    18481 agctgttgca accgcgtcgt gaaacgctgg agatggtggt tcgcgatctc tatccacgcg
    18541 gcggtcatgt tgagcaggcg actttctgga ctggcctgcg cccgatgacg ccagacggca
    18601 cgccggttgt cgggcgtaca cgctttaaaa atctgtggct gaataccggt cacggcacgc
    18661 tcggctggac gatggcttgc ggttccggtc agttgttaag cgatctgctc tcggggcgca
    18721 cgccagcgat cccgtatgaa gatctaagcg tagcgcgcta cagccgtgga tttacgccat
    18781 cacgcccggg ccatttacat ggcgcacaca gctaaggaaa cgagatgacc cgtccgatac
    18841 aggccagcct cgatctgcag gcattaaaac agaatctgtc cattgtccgc caggccgcgc
    18901 cgtacgcgcg cgtctggtcg gtggtaaaag cgaacgctta cgggcacggt attgagcgta
    18961 tctggagcgc gctcggggcc accgatggct ttgcattgct taatctggaa gaggcaataa
    19021 cgttacgtga gcgcggctgg aaaggaccga tcctgatgct ggaaggattt ttccatgctc
    19081 aggatctgga gatttatgac cagcaccgcc tgaccacctg cgtacacagc aactggcagc
    19141 tcaaagcact gcaaaatgcg cggctaaaag caccgttgga tatttatctt aaagtgaaca
    19201 gtgggatgaa tcggttgggc ttccagcccg atcgcgtgct taccgtctgg cagcagttgc
    19261 gggcgatggc gaatgttggc gaaatgaccc tgatgtcgca ttttgccgaa gcggaacatc
    19321 ctgatggaat ttccagcgcg atggcgcgta ttgagcaggc ggcggaaggg ctggagtgtc
    19381 ggcgttcgtt gtccaattcg gcggcgactc tgtggcacca ggaagcgcat tttgactggg
    19441 ttcggcctgg tattattttg tatggcgctt cgccgtccgg tcagtggcgt gatatcgcca
    19501 ataccggatt acgtccggtg atgacgctaa gcagtgagat tattggtgtc cagacgctaa
    19561 aagcgggcga gcgtgtgggc tacggcggtc gctatactgc gcgcgatgaa cagcgaatcg
    19621 gcattgtcgc cgcaggatac gccgacggtt atccgcgcca cgcgcctacc ggtacccctg
    19681 ttttagtgga cggcgttcgt accatgacgg tggggacagt ctcgatggat atgctggcgg
    19741 tcgatttaac gccttgcccg caagcgggta ttggtacgcc agttgagctg tggggcaagg
    19801 agatcaaaat tgatgatgtc gccgccgctg ccggaacggt aggctatgag ttgatgtgcg
    19861 cgctggcgtt acgcgtcccg gttgtgacgg tgtaacttgt tatcgctgga tgcgaccagc
    19921 gtcgtatcca gcgttataaa acacattaaa cttaagattc ggcttcttcc tccgctaccc
    19981 gaacaccaat cttcaacact tcattatctt ctttctcggc caccgtccag atcatcccgg
    20041 caaactctac ctggtcaccg acaaccggtg ctgcgcctaa caactgctgg acgatttcac
    20101 ccagcgtttg ctgcttatcg cgatattctc gcccgtcttc cagaccatat atcagcgcca
    20161 catcagcata tttggcgctg gcttcgagaa tgaagtcacc aaagaagcgt tgatccagcg
    20221 cgaccggcgg cgactggctg aacagtttac cgagcgccgg gagatcgcgt tcccgaccaa
    20281 ttacacacaa cacatcgcct tcgcgcagtc gggtgctgcc ggtgggatga agcaactggt
    20341 tatcacgaaa cagtgccgca atacgcgtct cttttggcat atgcaaatca cgcagtgccg
    20401 cgcccacgca ccatttatcg gcactcaatt gataaacaaa ctgctcccac ggattttccg
    20461 gatgaatatc caggccaacg cgtgacaccg gacgtcccac tggcggaacg accactttgg
    20521 cttttttcgc cgcccacgag agtgatgttc cctgcaatag cagtgaaacc aggaccacaa
    20581 agaaggcgac attaaagaac agtcgcgcat tctccagccc cgccatcatc gggaacacag
    20641 ccagaataat cggcaccgcg ccgcgtaatc ctacccagct gataaacacg cgctcacgca
    20701 gattgaagcc acggaacggt agcaatccgg caaataccga aagaggacgg gcgaagaaaa
    20761 tcatccatgc ggacaaaatg agcgccggaa tggcaatagg cagcagatcg cttgggttaa
    20821 ccaatagccc cagcaccagg aacatggcga tttgcgccag ccaggcgagg ccgtcgaaat
    20881 tttgcaggat gccgtagcgg ttgcgaatcg gccgattacc cagcagaaaa ccgcacagat
    20941 aaacagccag aataccgcta ccttccagcg ccgtggttaa agcaaaaatt aaaattccgc
    21001 cgcttaatgc cagcaatgga tataatccgg cgggcagggc gatgcgatta atcatttgca
    21061 gcagtaaata accaccgcca agcccgatga caattccgag gccaaattgc tgcagaatat
    21121 cgaccacgaa catccagctg acactgcttt catgctgctg gatcatcaca atcagggtaa
    21181 tcgtcagaaa gaccgccatt ggatcattac tgccggattc aatttccagc gtcgagccaa
    21241 cacgttcgtt aagcccctta ccacccagca gagaaaagac cgctgcagca tcggtggagc
    21301 cgacgatagc gccgattaat aatccttcaa tcaaatcaag attaaacagc cacgccgcca
    21361 tcatgccggt taaaccagag gtgataagca cgcccagcgt cgccagcgac agtgccggtc
    21421 ctaacgccac acgaaaggaa ctggcctgag tgcgcatccc gccgtcgagc aaaataatcg
    21481 ccagcgccag gttacttacc atgtaggcga agggataatt atcaaacggg ataccgccga
    21541 cgccatcaac ccccgccagc atgccgatcg ccagaaaaat aaccagaatg ggaatgccaa
    21601 gacgggaaga aaatgaacta agtaaaatac tgctggtgac aaggatggat cctaagatga
    21661 aaaggctaat tattgttgtg gcatccaacg gtcggttact cctgattacg ctgtctctta
    21721 tataaaccct accatattag cggcagagac agcgttttac ttagccctga agcgtgattt
    21781 ttttacattt taagaacagg atgaccgcta atcgtcaact gagtgccttc ctgggtgtta
    21841 ttcagaatgg catgtgcgcc caacggcagc gtaaccgtgc gttgttcatg accaaaatcg
    21901 aggccggtaa tgagtggaat cgacaggcgg gaacgcaaaa acgcgtacac tgactccagg
    21961 ttgtaacctg cgtcataatc attaggcgtg ctgccgctaa agctaccgag aataatcgcc
    22021 ttctgacgtg gcaaaattcc agcatgatag agctgcaaca gcatacgttc gacgcggaaa
    22081 gggtgctcgt taatatcttc cagcaccaga ataccgttct caatttttgg catccacggt
    22141 gtaccaatca gtgaaatcag catcgcaaga ttgcctcccc acagcgtgcc ttcggtctga
    22201 catgtcggac cttccccttg ccattcaatg gtgaacgttt tattgcgtaa cgccagccag
    22261 aagtggtgct cggtaaaggc atttagttca tccgcgccaa agtttgctac cagcatgggg
    22321 ccgctaaagg tgatgacatt gccttgcgcc agaagaccgc actgaatggc ggtaaaatcg
    22381 ctatgtccgc aaatgagtaa cgggtcatgt tgttggcgag ccaccagtgc ctgccagtca
    22441 atatctgcca gtaaacgact ggcaccgtaa ccgccgcgta cagacagcac gatggtgttg
    22501 ggggtagtca gtctggcaag agaattgaga tcttccagac gctccgtttc cgtacccgca
    22561 aaacgctcac aacgacgggc aataacctcg acgttattga cctgatgccc cgcgtcggta
    22621 aggcgctgga taccacgcag cgcggcgtgc tgtttaatgc agtaacccga tggggcaatt
    22681 aagtgaaaca gagacatggc aattccttgc tgacaacaga aacgaaatgt atatcatgcc
    22741 gcttaggtgt gccgttgtca cctcaacggc gattccaggc tataaggata gaagaagtga
    22801 aattgagatg gtttgccttt ttgattgtgt tattagcggg ttgttcatca aagcatgact
    22861 atacgaaccc gccgtggaac gcgaaagttc cggtgcaacg tgcgatgcag tggatgccaa
    22921 taagccagaa agccggtgca gcctggggcg tcgatccaca attgatcacg gcgattatcg
    22981 ctatcgaatc gggtggtaat ccgaacgcgg tgagtaaatc gaatgccatt ggtttgatgc
    23041 agataaaagc ttcaacctcc ggacgtgatg tatatcgtcg tatgggctgg agtggtgagc
    23101 cgacgaccag cgagctgaaa aatccggagc gtaatatttc aatgggggcg gcttacctga
    23161 atattctgga aaccggcccg ctggcaggca ttgaagatcc gaaggtactg caatatgcgc
    23221 tggtggtgtc atacgctaac ggggcaggtg cgctgctacg gactttctcg tcagatcgga
    23281 aaaaggcgat cagcaaaatc aacgatctgg atgctgatga gttcctcgat cacgtagcgc
    23341 gaaatcatcc tgcgccgcag gctccgcgct atatctacaa acttgagcag gcactggacg
    23401 cgatgtaaat cagtcgcgca cattgtccgc ttttttccgg gcttctcgct cgagagagaa
    23461 aataatccgc tgtaattgcc gctccaccgt cgggctgacg ttaagaaaac ggaagctcag
    23521 acggggagtg gtgatggttt catttttgcc atcaatcact ttgcgctcgc tgatggagat
    23581 taactgggcg tcaaagtgaa aaacacccca ttgccccatg ttgacttcaa tctgagcgaa
    23641 gcgcatgcct tcgtgtaatt cggcaggctt tgctgtttcc agtaatgcgc ccatgccgcc
    23701 taacgacaaa tcatacaggc ggaaacgtaa cgtactgtta tccgccagtt tggtctggca
    23761 aaaataaggc ggatggagtg gggcggtgat gcggaaatat cggcgtcgtt gtacaaacca
    23821 taaggtggga ggcggtacgg taataaatgc cggaagctgc aagtattcac tctgctgtag
    23881 ttgttcaaca gtaaactcga ctttcgcacc ctgagtttcg gcggtaatgg taatgtgctg
    23941 cgcctttagc acggcgatgt tgtcttcggc ctggctgccg aaatccagca ccagtttatc
    24001 cggagttatt gccagtattt tgctgatcag ctgcccgcca ttccagctga tacgcaaagg
    24061 aattgcggct ttgttcaaat cgcgtaacac gcccaggacg gctaacggat tttgtttcag
    24121 gaactgctca tggtaatgac tcacgcggaa aaactcctga ctgacaaact gtctttggtt
    24181 tatcggtcgc agttaacaaa acttaataca aatgcgcgaa tattttttac atgttgttct
    24241 taaatcagcc gacatacgcc cagcatcgat cccgcccttg cctatactta gagcgttgac
    24301 gtaagcattt cttgcgtccg attcatcgaa acgagggcct gaacatggga attattgcct
    24361 ggattatttt tggcctgata gccggtatta tcgccaagct aatcatgccg gggcgtgatg
    24421 gtggtggatt tttcctgacc tgtattcttg ggatcgtcgg tgcggtggtc ggcggctggc
    24481 tggcgaccat gtttggcatt ggcggctcca tcagcggttt taatttgcac agcttcctgg
    24541 tggcggtggt gggcgctatc ctggtgctgg gcgtattccg cctcctgcga agagaataag
    24601 attttcataa ggcggatagc gatacagatg ccgctatccg ctttcacatc agaacgtata
    24661 ctcgatacct gcccagaaat tgcgaccttc ttcaataaac ccttcactgt aggcatattt
    24721 ggtatcaaac aggttattga ccgacgcatt gacgctgaag ccatgaccta aggtgtaatc
    24781 ggctcgaatg tgagtcaccg caaaaccggc ggctttttgt gaaccgtcat tgttgctgta
    24841 gctggaggaa cgcgcctctt ccgacagcgt tacgcttaac ggctcccacg gtttgagagt
    24901 catccatgcg gtcattgtct gcgttggcag atcggttatc ttgccgatgt ctttgcgttt
    24961 ggcatcagcg tggatcaggg cgtagctcaa tcctacatcc agtatattgc tgattttccc
    25021 cttaataccg gcatccagac cgctgtaatc caccgtgccg ctgttctgat tttgaatggt
    25081 atcggcatcg atattgtgcg agaggatggc atcactcacc cggttatagt aaacgctgac
    25141 ctcaaatccc cagtcgtgcg tgaaggcacc attccaggtt aaatccaccc cgcgcgcgcg
    25201 ttccggcttg agctgcgggt taacaatcgc tatctggtta tacgcaggtt tggacgtggt
    25261 atagcgttct ttcagcgtcg gaaagcgtgt gcggtcatag tacgaaagcg ccagcgtgtc
    25321 ttcattggca aagtggtatt tccccatcac ctgccagtta aaagctgact gattgttgtc
    25381 gtcataatgg gtgatactgc catctttctc atgttttttc gcttctacgc tatcgcgcca
    25441 gtcatagctg attccagcca cgacatcgac attatcggca gcagcccatt gatattcact
    25501 ggcgagcgac caggtacgat cttcatagcg atcgtaagcg gcgtgcggcg cacctttttc
    25561 ccggtgtacg tcatctttcc agttaacggc aaacgacagc agatcgtttt cacgcacatc
    25621 ggctgccagt tgtaacccgg caccgtcgct gtaatcggaa taatggctgt agctgccttt
    25681 tttatttttc aaatcagcca gcgagttgta catcattagc gtattttcaa aggtgtcgcg
    25741 atacagccga cttttcaggg taaaacgatc gtttagttgg gtcgttccct gataataaaa
    25801 actttctttg tcatactctg gccactgcca gtagcgtgat ttctgaccac tatttccact
    25861 gtatggcggg ttatcttttt caccatcctg cttaatgtaa gtcaatgtgt attcatcgtt
    25921 ttcgcgtggt gtaaaaccga gcttcacaat gccgcgttta tcatcagccg atgaattaat
    25981 catcttgccg tgtttgcctg caatatcatt atttacacca tgcggcaggc cgagaaaatc
    26041 ctgctttagc tggctaccgc tgacttgcaa ataccccaga tcgctgctgg cggcaaatga
    26101 agcatgcata tcgtaggcat tgtcttggct acggctccat ccctggcgat atcccagatt
    26161 tgcttccaga ggttttgttg gcttctgggt ggtgatatta atggctccgc ccatctgatt
    26221 aggcccctga agcagcgacg aatacccttt ggaaacttca actgccctca aattgttggt
    26281 cagaatccgc gccagatcga ggttgccgtc atagggaaca taaatgggca caccgtcgaa
    26341 atagaccggt acctgacgac tatcaaagcc acgaactttg acctgttctt cgttgcggct
    26401 acctgacttt tgcagcacca cgccggggac gacacttaat gcctgagcga cattctgttt
    26461 atccagggcc ttcatggtgg gttgatcgag aacggtggtc gtcgttgatg ataccggact
    26521 ggaccatacg gtcagcgtgt cgctctcctg agccgctacg gcctgctggg taaaggcaag
    26581 tgataatact gtgcagagat aacgtttttt gagtctcata gaataaattc cgtttaacaa
    26641 agaattaaat agaggctgcg ggttgcagac tgactttaat gtcagcaggt gccgcgtaat
    26701 aaggcgcgga ggtaataaaa aggcgaatgc cgcagtcgag gtaatttttg agtgttgtca
    26761 gattaattcc gccggtcaga gccagcgtgc agtggggagc cagcgacggt gctatttgcg
    26821 caatttcagt tgcctgttgc ggactaaatt tgtcgagctg aagtacgtct ggttgcgcgc
    26881 gtaacgcggc gattgcctct ttcggcgtgt cggcttcaac aactattttc ttctcgggtg
    26941 cgtggcgacg taactgattg attgcgcctg accagtcctg attgtcatga agaaaatggc
    27001 ggtggttggc aaacaataat atggtttccg cacatccggc acgatgaatc agtcctccgg
    27061 cagccagaat tgcctgcgag gccagtaagc gggtgcccgg aattgctttt cgggtgcagg
    27121 cgatattgcc atcagggtaa cgttcacgaa gtaacgccag catttgagcg agataatcag
    27181 aaacaccgca actccactcc agcacattct ggactgcctt ccatccctga tgaagtgctg
    27241 cggcattacc ttgcgcacgg attagccgct gacccgcgtt cgcttgtgaa ccgtcgctga
    27301 ccgcgtcatc aatagttaat cccagcgtag ttaacatctt acacgcgaca gaaattccgc
    27361 tgacgcagcc gccctgacgg agaaaaaact ctatatagcc atgctggtgt ccaatattta
    27421 atgctcgcgt ggtcaggtca ccgccctgga tatcttccag aagtaacgca tcgatttgtg
    27481 cctgagaaag gaaaatcatc gcagtgtctc ctgcggtacg ctatcccacc agatgagcgc
    27541 ccagtcgcgg ctggcagggg cgagggcacg agcatcttgt tgttgatacc agcgatataa
    27601 ccgctcacgc tcatcgtcgt taagcgcgcc cagactccag ctgacgttct gctcaaaacg
    27661 ttcccaggtg ctgttatcct gttggcaatt ttgtccgcgg ataaaatcaa cgtgagcata
    27721 aattcccatc tgatacagga cgttgagcgc aaagatatag ttgggtaatt cgaccacttc
    27781 ccgaccagcc gcccgctgaa tggctggaga gacgaacgag gtgctgacca gatgagtcgt
    27841 atagacgcga agccgtgcct gattattcag tttgctcatt gcctggcgca tatcagccac
    27901 cagagtggag cgggaagcca cggcaatatc gcagcgtggc aggtcactcc agtcctcttc
    27961 ccaggcgcgc tggatccagt ggacattgtc tgcctttagc gcggcggcgc gacgggcggc
    28021 aacgttcagc atcccctgac tgtaatcaac gccataaatc gtcgtgagtt tgtccgccag
    28081 cgccagcgat acggtacccg gtccgcagcc catatcgaat aaggtttgcg cgccttgcag
    28141 gtcaattttt gaggtcagtt gttgcaggta gctgtccgtc ggcgaggcgc aattttccgc
    28201 cattttttcc gcccgttgat cccagtgatc cggcgttttt tctgtgcgat gggccagcct
    28261 gagttgctga aggtaaaggt ctgcaaaatc aatatcgtca atgagcatga tttttcctta
    28321 gtgcttaatt gcagagaggt gatggtgaat ctggtcggca gaaacccggt acagggcagc
    28381 cagttttttc gttgttaact gttcggtcgg taagccttgt gttactcgtc catctggctc
    28441 tacctggata atgctgtcgg caatggcgtt tgcatggagc ggatgatgag tcgacattaa
    28501 cattgtcatg ccgttatttt tgagctgcgc cagcgtgtcc agaagttgga tctgatgacc
    28561 gaaatcgagg ctggaggcag gctcatccag taataacaag cgcggttgct ggacgagtgc
    28621 acgggcgatt aaagccagct gacgttcacc gccgctaagc gtattccagc gtcgtgtcgc
    28681 cagatgcaaa atgcccagct tttccagttg ctccgttgct ttcaggcgtt cttgcttacc
    28741 gggaacggaa aaagcgccta tggtgggggc caggcccatc aacaccatat ccagcacact
    28801 aaaagcaaaa atgccgtcgt gcgcctgcgg cacccaggca atagcctgcg cgcgctggcg
    28861 atggctgagt gtctgcacag ggaggctatc cagtaagatc tcgcctttta ataacggtat
    28921 aacgccaaga atggagcgca tgagtgtggt tttgccgcaa ccattggctc ctaacagaca
    28981 acaaattgtt ccttgcgaca gggtgaagga cacgtcgcgt agcaccggat ggcgggagta
    29041 acccagagac gcatgacgga cggcaagcaa agtcactggc gacctccgcg taacaacaaa
    29101 aacaggaaaa acggtgcgcc aacaaaagcg gtcagaatgc caagtgggat ttctgttgtt
    29161 ccaatgctgc gtgccagcgt atcggtaagc aggagcagaa tcgcgccagt acacattgcc
    29221 atgggcaaca gctgctgatg gttatgaccg gtcaacaagc gggcaatgtg cggaacgacc
    29281 aggccgaccc aaccaattat gcccgctatt gctaccgtgc tggcggtgat gagtgtggca
    29341 cagacaatca atccaaagcg cagacgtgtc acgttcagtc ccagcgaacg ggcttcgtcg
    29401 tcggaaagcg tgagtaaatt catgcgccag cggagaaaaa acaggggcaa ggagcctatg
    29461 agaatgatcg gtgcagcata gcagagatcg cgtaaggtca cggtggacag cccccccagc
    29521 aaccagaagg taattgacgg taactgggta taaggatcgg ccagcgtttt tattagcgat
    29581 atccccgctc cgcacaacgt tcctaacgca atgcctacca gcaccagggt taagatcgga
    29641 tcgtggcggg tcacccgacg cgtaatcaac cagacgcccg cgaccaccat cagcccgccg
    29701 caaaatgcgt acagctggat gtaaacaatg gataatcccc acaggattgc cgcgcaggca
    29761 ccaagtccgg caccggcgga gacgcctaaa atatccggtg acactaaggg attacggaac
    29821 attccctgat aggtggttcc ggcccctgcc agcgccgcgc ccagaaacag cgcagcaaga
    29881 atacgcggca cgcggatttg ccagaagaca atttgttcct gcacaggaac attttcttga
    29941 cgaagaatga gcgccagcag ttgctgcata tcgagatgat aagccccact tacggcggca
    30001 aaagtgatac tgatagctgc cagcagcaaa atacatccgt ttacggttct cattagctcg
    30061 ccaccaattt ttgatagtcg gcgtcactga gtgaacaatg ccagaacagt tgggcgtaat
    30121 gctgtatgtc acttttaaac tggcgattga tggcgggatc cagccaggcg tgaagtctgc
    30181 gtaatcccag aagacggttg attcccggcg gggcatccag ccagccaaag ggcaggccac
    30241 ttaaaaataa gatgcgttgt tccgcaaccg ctttcacgcc ctgccagaga ggatcacgac
    30301 gaataaaatc tgcagtaacg gcctcctgaa ccagaataat atccggctgc cagcgcagga
    30361 gattttccat ggaaacctgt gtcagaccgt ggcgatcggc tatttgcgcg acgttgtgca
    30421 aacctaataa ttccgccgcc tcggtatgca acgaaccctg taagcccgtt tccagccctc
    30481 ggggaccgcg cgcagcataa aagctgagat tagcggcggg tgaggtggcg aatgcctgag
    30541 cttcaccaac gaagcggctg gcgagattgg cttgttctgc ggcgcggtgc tcttcgccta
    30601 acgttttgcc aagcgttgtt aactgttctg ctgattgttc cagtttcccg ttaagcaata
    30661 accagggtat ttgtgtctgt tcgctaacct gccgtgcctg ggagatccag gtttcatccg
    30721 tattgccgca atcaacgacc aaatcgggat gtaacgccat aagcccttcc agcgagagtg
    30781 tgctggcgcg cccggcgagt cgtcccagcc tggggaactg gcgaatgtgc tctggcaatg
    30841 gaattaatgc ctgacgggca aaatcaaaag aggaaaaacc gaccattttt tctggcgcga
    30901 cagccagcaa tagtaaatcg gcgggcgcgc ctgcgctgac gatgcgtgta atgttttggg
    30961 cttcgggaag cgtccgtgag gcccagaatt tgactgattg tgcaaaggac ggcagatgat
    31021 gcaggaacaa tgtcgacgca agaatctgtg taaacctccg tctggttagt gacataagta
    31081 atatcctgat aattaaaacc gctatatact tacacatata gcgaaaaaga tcatgtttga
    31141 tgtcaaaatt tgtctaaaga gacagaatga tttgcttgtt tcttaaataa cactttgttc
    31201 cacaagaaaa ttgtcaggga ggatggggag tgg
//
LOCUS       DAZGJT010000049        31182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZGJT010000049 DAZGJT010000000
VERSION     DAZGJT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.049"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..252
                     /locus_tag="ACHKFK_002495"
     CDS             <1..252
                     /locus_tag="ACHKFK_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HFO9052812.1"
                     /translation="ITLRRSHKEFHPDNNFTIDAINSFVMRIQRSNADGDCLTGLFNV
                     SENIQHINITDLHGRDLISEVDILGNEITLHPWQVMWIK"
     gene            266..1558
                     /gene="ycjN"
                     /locus_tag="ACHKFK_002496"
     CDS             266..1558
                     /gene="ycjN"
                     /locus_tag="ACHKFK_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9052813.1"
                     /translation="MIKSKIVLLSALVSCTLISGCKEENKNNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRQAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRNLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLISELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQTSQKKLDELVEQR"
     gene            1579..2460
                     /gene="ycjO"
                     /locus_tag="ACHKFK_002497"
     CDS             1579..2460
                     /gene="ycjO"
                     /locus_tag="ACHKFK_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9052814.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            2447..3289
                     /gene="ycjP"
                     /locus_tag="ACHKFK_002498"
     CDS             2447..3289
                     /gene="ycjP"
                     /locus_tag="ACHKFK_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO9052815.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPVIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSEHFIKSGLTAG
                     GVKG"
     gene            3320..4372
                     /gene="ycjQ"
                     /locus_tag="ACHKFK_002499"
     CDS             3320..4372
                     /gene="ycjQ"
                     /locus_tag="ACHKFK_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO9052816.1"
                     /translation="MKKLVATAPRVAALVEYEDRAISANEVKIRVRFGAPKHGTEVVD
                     FRAASPFINDDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVNDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPITRRCDIARRHGADFCLNPIGADVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            4391..5179
                     /gene="ycjR"
                     /locus_tag="ACHKFK_002500"
     CDS             4391..5179
                     /gene="ycjR"
                     /locus_tag="ACHKFK_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO9052817.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQAAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHAHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAENPAQAYRDSLAWLRTC"
     gene            5189..6244
                     /gene="ycjS"
                     /locus_tag="ACHKFK_002501"
     CDS             5189..6244
                     /gene="ycjS"
                     /locus_tag="ACHKFK_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9052818.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELEAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKQGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVTAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            6241..8465
                     /gene="ycjT"
                     /locus_tag="ACHKFK_002502"
                     /pseudo
     CDS             6241..8465
                     /gene="ycjT"
                     /locus_tag="ACHKFK_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
     gene            8462..9121
                     /gene="pgmB"
                     /locus_tag="ACHKFK_002503"
     CDS             8462..9121
                     /gene="pgmB"
                     /locus_tag="ACHKFK_002503"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO9052819.1"
                     /translation="MKLQGVIFDLDGVITDSAHLHFQAWQQIAAEIGISIDAQFNEFL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIHNLL
                     VDLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTESLTWPRLSAFWQN
                     V"
     gene            9135..10217
                     /locus_tag="ACHKFK_002504"
     CDS             9135..10217
                     /locus_tag="ACHKFK_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9052820.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQESLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIC"
     gene            10262..11167
                     /gene="ompG"
                     /locus_tag="ACHKFK_002505"
     CDS             10262..11167
                     /gene="ompG"
                     /locus_tag="ACHKFK_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO9052821.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEEKNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRISLAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLESD
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETLALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(11218..12216)
                     /gene="ycjW"
                     /locus_tag="ACHKFK_002506"
     CDS             complement(11218..12216)
                     /gene="ycjW"
                     /locus_tag="ACHKFK_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9052822.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
                     YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQRSCELLFQLIAGKPSPQNITVATHMSLK"
     gene            12372..13769
                     /gene="ycjX"
                     /locus_tag="ACHKFK_002507"
     CDS             12372..13769
                     /gene="ycjX"
                     /locus_tag="ACHKFK_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO9052823.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     SIHAGARLPLLSAVREERLLGVKRIPQRDFGITRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYFSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVIDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            13766..14827
                     /gene="ycjF"
                     /locus_tag="ACHKFK_002508"
     CDS             13766..14827
                     /gene="ycjF"
                     /locus_tag="ACHKFK_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO9052824.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            14975..16516
                     /gene="tyrR"
                     /locus_tag="ACHKFK_002509"
     CDS             14975..16516
                     /gene="tyrR"
                     /locus_tag="ACHKFK_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO9052825.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSTLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRASKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(16560..17066)
                     /gene="tpx"
                     /locus_tag="ACHKFK_002510"
     CDS             complement(16560..17066)
                     /gene="tpx"
                     /locus_tag="ACHKFK_002510"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO9052826.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            17185..18150
                     /gene="ycjG"
                     /locus_tag="ACHKFK_002511"
     CDS             17185..18150
                     /gene="ycjG"
                     /locus_tag="ACHKFK_002511"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO9052827.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVTTAQTIVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            complement(18125..18853)
                     /gene="mpaA"
                     /locus_tag="ACHKFK_002512"
     CDS             complement(18125..18853)
                     /gene="mpaA"
                     /locus_tag="ACHKFK_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO9052828.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASHESGLI
                     LAGTHGDENSSIVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(19144..19782)
                     /locus_tag="ACHKFK_002513"
     CDS             complement(19144..19782)
                     /locus_tag="ACHKFK_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9052829.1"
                     /translation="MKNILILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            complement(19853..20773)
                     /gene="ycjY"
                     /locus_tag="ACHKFK_002514"
     CDS             complement(19853..20773)
                     /gene="ycjY"
                     /locus_tag="ACHKFK_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9052830.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVDAGSDARTSDISSGEYATMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPIQIVAGSQAGSKWMSDDLYDRASSKDKRYHIV
                     EGANHMDLYDGKVYVAEAISVLAPFFEETL"
     gene            20911..21810
                     /gene="pgrR"
                     /locus_tag="ACHKFK_002515"
     CDS             20911..21810
                     /gene="pgrR"
                     /locus_tag="ACHKFK_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO9052831.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALTSLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSAPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            22147..23760
                     /gene="mppA"
                     /locus_tag="ACHKFK_002516"
     CDS             22147..23760
                     /gene="mppA"
                     /locus_tag="ACHKFK_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO9052832.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESSKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(23811..24842)
                     /gene="ynaI"
                     /locus_tag="ACHKFK_002517"
     CDS             complement(23811..24842)
                     /gene="ynaI"
                     /locus_tag="ACHKFK_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO9052833.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVTEIGWRITKIKTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPDQGR"
     gene            25086..25343
                     /gene="ynaJ"
                     /locus_tag="ACHKFK_002518"
     CDS             25086..25343
                     /gene="ynaJ"
                     /locus_tag="ACHKFK_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO9052834.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(25393..26343)
                     /gene="uspE"
                     /locus_tag="ACHKFK_002519"
     CDS             complement(25393..26343)
                     /gene="uspE"
                     /locus_tag="ACHKFK_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO9052835.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(26495..27247)
                     /gene="fnr"
                     /locus_tag="ACHKFK_002520"
     CDS             complement(26495..27247)
                     /gene="fnr"
                     /locus_tag="ACHKFK_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO9052836.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(27442..27957)
                     /gene="ogt"
                     /locus_tag="ACHKFK_002521"
     CDS             complement(27442..27957)
                     /gene="ogt"
                     /locus_tag="ACHKFK_002521"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO9052837.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKDGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGVTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(27968..28120)
                     /locus_tag="ACHKFK_002522"
                     /pseudo
     CDS             complement(27968..28120)
                     /locus_tag="ACHKFK_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28470..29285)
                     /locus_tag="ACHKFK_002523"
     CDS             complement(28470..29285)
                     /locus_tag="ACHKFK_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9052838.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRIWAGESVWLVHASSALWLPPQMPHKFVATGNILLKTVLVSEAESETLGKVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHDYAITRQMI"
     gene            29382..30410
                     /locus_tag="ACHKFK_002524"
     CDS             29382..30410
                     /locus_tag="ACHKFK_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9052839.1"
                     /translation="MIISKKQLIGVVAIGILLAGVVFFIWWVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYCANVARLEAKIKSSKANLES
                     IQATIAMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTLVRAPID
                     GIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQIAGMKPGMKAEIRADILKG
                     EVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVRIVFDDAKELKQLLRPGLS
                     VTVSVDER"
     gene            30407..31182
                     /locus_tag="ACHKFK_002525"
                     /pseudo
     CDS             30407..31182
                     /locus_tag="ACHKFK_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
BASE COUNT         7754 a         7343 c         8137 g         7948 t
ORIGIN      
        1 attacacttc gtcgcagtca taaagagttt catccggata ataatttcac cattgatgcg
       61 attaattcat ttgtaatgcg aattcaaaga agcaatgcgg atggcgattg cctgacggga
      121 ttgtttaatg tcagtgaaaa tattcagcat ataaatatta ctgacctgca cggtcgggat
      181 ctgattagtg aagttgatat attgggtaat gaaataacac tgcatccctg gcaggttatg
      241 tggattaaat aaaaaggaac atttcatgat taaatcaaaa atcgtgctgt tatcagcact
      301 ggtttcctgc accctgattt caggctgtaa agaagaaaat aaaaataatg tatccatcga
      361 atttatgcat tcttcggtgg agcaggagcg ccaggccgtc atcagcaaat tgattgcccg
      421 ttttgaaaaa gaaaacccag gtatcaccgt aaaacaagtg cccgtggaag aagacgccta
      481 taacaccaaa gtcattaccc tttcacgtag cggttcgctg ccggaagtga tcgaaaccag
      541 ccatgactac gccaaagtga tggacaaaga gcagcttatc gatcgccagg cggttgccac
      601 ggtcatcagc aacgtcggtg aaggcgcgtt ctacgatggc gtactgcgga ttgtgcgcac
      661 cgaagatggc agcgcctgga ccggtgttcc tgtcagcgca tggattggcg ggatctggta
      721 tcgcaaagat gtgctggcaa aagctgggct ggaggagcca aaaaactggc agcaattgct
      781 ggacgttgca cagaaactga atgacccggc gaataaaaaa tacggtattg cgctgcctac
      841 agcagaaagc gtgttgacgg aacagtcttt ctcccagttt gcgttatcca atcaggctaa
      901 cgtctttaac gccgaaggca aaatcaccct tgatacacca gagatgatgc aggcgctgac
      961 ctattaccgc aaccttgctg ccaacaccat gccaggttct aacgacatca tggaagtgaa
     1021 agacgctttt atgaacggca ccgcgccgat ggcgatttac tccacctata tccttccggc
     1081 tgtgattaaa gaaggcgatc cgaaaaacgt cggtttcgtg gtgccaacgg agaaaaactc
     1141 tgcggtctac ggcatgttga cctcgctgac cattaccgcc gggcaaaagg ccgaagagac
     1201 ggaagcggct gaaaaatttg tcacctttat ggagcaggca gacaacattg ccgactgggt
     1261 gatgatgtcg ccaggcgcgg cgctgccggt gaacaaagcg gttgtcacta ccgccacctg
     1321 gaaagacaac gacgttatta aggcgctggg tgagctgcca aatcagttaa tcagcgaact
     1381 gccaaatatt caggtctttg gcgcagtagg ggataaaaat tttacccgca tgggcgatgt
     1441 gacgggttct ggcgtggtga gctccatggt gcataacgtc accgtgggta aagccgacct
     1501 ccctggcacg ctgcaaacga gccagaaaaa gctggatgag ttggttgaac agcgctaaac
     1561 ccaggacagg aatccgcaat gaacaggctt ttttcaggtc gttccgatat gccctttgcg
     1621 ctgttgcttc tcgcgcccag cttattactg ctgggcggtc tggtggcgtg gccgatggtg
     1681 tcgaatatcg aaatcagttt tttacgtctg ccgcttaatc ccaacatcga gtcaacgttt
     1741 gttggggtga gcaactacgt gcgtattctc tccgatcccg gcttctggca ctcgctgtgg
     1801 atgacggtct ggtataccgc gcttgtggtg gcgggcagca ccgtgctcgg gctggccgtg
     1861 gcgatgtttt tcaaccgcga gttccgcctg cgcaaaaccg cgcgttcgct ggtgatcctc
     1921 tcctacgtaa cgccgtctat ttcgctggtg ttcgcctgga aatacatgtt caacaacggt
     1981 tacggcattg tgaactacct cggcgtcgat cttctgcatc tctatgagca ggcaccgctg
     2041 tggttcgata atccgggcag tagctttgtg ctggtggtgc tgttcgccat ctggcgctac
     2101 ttcccgtatg cctttatttc gtttctggcg attttgcaga ccatcgacaa atcgctgtac
     2161 gaagcggcag agatggatgg cgctaatgcc tggcagcggt ttcgtatcgt cacgctgccc
     2221 gcgattatgc cggtcctggc gacggtggtg accctgcgca ccatctggat gttctacatg
     2281 ttcgcggatg tttatctgct gaccaccaag gtcgatattc tcggtgtcta tctctacaaa
     2341 accgcctttg cctttaatga cttagggaaa gcggcggcga tctcggtggt gctcttcatc
     2401 attattttcg ctgtcattct gctgaccaga aaacgggtga acctcaatgg caacaaataa
     2461 acgcacactc agtcgcatcg gtttttactg cgggctggcg ctgtttctca tcatcacgct
     2521 gtttccattt tttgtgatgc tgatgacctc gttcaagagc gcaaaagagg cgatctcgct
     2581 gcatcctacg ctgctgccac agcagtggac gctggagcat tacgtcgaca tttttaatcc
     2641 ggtgattttc ccgtttgtcg actacttccg taacagcatg gtggtgtcgg tggtttcatc
     2701 cgtggtggcg gtatttctcg gcattctcgg ggcttacgcg ctttcccgcc tgcgctttaa
     2761 agggcggatg accatcaacg ccagctttta cacggtgtac atgttctccg gcattttgct
     2821 ggtggtgccg ctgttcaaaa tcatcaccgc acttggcatt tacgacaccg agatggcgct
     2881 gattatcacc atggtgacgc aaaccctgcc caccgctgtg tttatgctga aaagctactt
     2941 cgacaccatt cctgatgaga tcgaagaagc ggcgatgatg gatggcctca atcgtctgca
     3001 aattatcttc cgtatcaccg tgcccctggc gatgtccggg ctgatatcgg tgtttgtcta
     3061 ctgctttatg gtggcgtgga acgactatct gtttgcgtcg attttcctct ccagcgccag
     3121 caatttcacc ttaccagtgg gcctgaacgc gctgttcagt acgcctgatt acatctgggg
     3181 acggatgatg gcggcctcgc tggtgaccgc attgccggtg gtgattatgt atgcgctttc
     3241 ggaacatttt attaagagtg gtttgaccgc cggtggcgtg aagggctaaa gcggccagtt
     3301 tcttgacaag gagttttaca tgaaaaagtt agtagccaca gcaccgcgcg ttgctgcgct
     3361 ggttgagtat gaagatcggg cgatttcagc caatgaagtg aagatccgcg tgcgattcgg
     3421 cgcgccgaaa cacgggacgg aagtggttga tttccgcgcc gccagcccgt ttattaatga
     3481 cgactttaat ggcgaatggc agatgttcac cccgcgtccg gcagatgcgc cgcgcggcat
     3541 tgagtttggc aagttccagc ttggcaacat ggtggttggc gacattatcg agtgcggcag
     3601 cgacgttaac gactacgcgg tgggcgacag cgtatgcggc tacggcccgc tctccgagac
     3661 ggtcatcatt aacgcagtga ataactacaa gctgcgcaaa atgccgcaag gcagctcctg
     3721 gaaaaacgcc gtctgctacg acccggcgca gtttgccatg agcggcgtgc gcgatgccaa
     3781 cgtacgcgtg ggcgattttg tggtggtggt cgggcttggc gcgattggtc aaattgccat
     3841 tcaactggca aaacgcgctg gcgcgtcggt ggtgattggc gtcgatccta ttacccgtcg
     3901 ttgtgatatt gcccgtcgcc acggtgcgga tttctgtctt aaccccattg gcgctgacgt
     3961 aggtaaagag atcaaaacgc tgaccggcaa gcagggtgcc gatgtgatta tcgaaaccag
     4021 cggttacgcc gacgcgctgc aatcggcgct ccgcggtctg gcctacggcg gcaccatctc
     4081 ctatgtcgcg tttgccaaac catttgctga aggttttaac ctcggacgcg aagcgcattt
     4141 caataacgcc aaaattgttt tctcccgcgc gtgcagcgaa ccgaacccgg attatccgcg
     4201 ctggagccgt aagcgtattg aagaaacctg ctgggaactg ctgatgaacg gttatctcaa
     4261 ttgcgaagat ttaatcgatc cggtggtgac ctttgccaac agcccggaaa gctacatgca
     4321 gtatgtcgac cagcatccgg aacagagcat caaaatgggc gtcacgtttt aattcaaagg
     4381 aacgaaaaga atgaaaatcg gcacacagaa tcaggcgttc tttccggaaa acattctgga
     4441 gaaatttcgt tatatcaaag agatgggttt cgatggtttt gagattgacg gcaaactgct
     4501 ggtcaacaat ctcgaagaag tcaaagcagc aatcaaagaa accggtttac cggtgaccac
     4561 cgcctgcggt ggctatgacg gttggattgg cgactttatc gaagagcgtc gacttaatgg
     4621 cttaaagcag atcgaacgca ttcttgaagc gctggcagaa gtgggcggta aaggtatcgt
     4681 cgttccggct gcatggggca tgtttacctt ccgcttaccg ccgatgacct cgccgcgtag
     4741 cctggacggc gaccgcaaaa tggtgagtga ttccctgcgc gtactggaac aggcggccgc
     4801 acgtaccgga accgtggtgt acctcgaacc gttaaaccgc tatcaggatc acatgatcaa
     4861 caccctcgcc gatgctcgtc gttacatcgt cgaaaacgat cttaaacatg tacagattat
     4921 cggcgatttc tatcacatga atatcgaaga agataacctg gcgcaggcgt tgcacgacaa
     4981 ccgcgacttg ctcggtcatg cgcatattgc cgataaccat cgctaccaac cgggcagcgg
     5041 caccctcgat ttccacgcgc tgtttgaaca gctgcgcgcc gataactatc agggctatgt
     5101 ggtgtatgaa gggcgtatcc gggcagaaaa tcctgcccag gcgtaccgtg attcgttggc
     5161 ctggttgcgc acctgctaag aggtctttgt gaaaagtgca atgacaagct ctccgctgcg
     5221 agtcgcgatc ataggcgcgg gccaggtggc ggataaagtt catgcttcgt actactgcac
     5281 ccgcaacgat ctggaactgg aggctgtctg tgacagccgc ctttcccagg cgcaggcgct
     5341 ggcagaaaaa tacgggaatg catccgtgtg ggacgatccg caggccatgc tgctggcggt
     5401 gaaacctgat gtggttagcg tctgctcacc taaccgtttt cattacgaac ataccctgat
     5461 ggcgctggaa gcgggctgcc atgtgatgtg cgaaaaaccg cccgccatga cgccagaaca
     5521 ggcgcgggaa atgtgcgata ccgcgcgcaa acagggcaag gtgctggcct acgactttca
     5581 ccatcgtttt gcactcgata cgcaacagct gcgtgaacag gtgaccaacg gcgttttggg
     5641 agagatttac gttaccaccg cccgcgccct gcgtcgctgc ggtgttcccg gctggggtgt
     5701 ctttactaat aaagaattgc agggcggcgg cccgctgatc gacatcggca ttcatatgct
     5761 ggatgcggcg atgtatgtgc tgggttttcc agcggtgaaa agcgtgactg cgcatagctt
     5821 tcaaaagatc ggcacgcaaa agagctgcgg tcaatttggc gagtgggacc cggcaaccta
     5881 cagcgtcgaa gattcgctgt ttggcaccat tgaatttcat aacggcggca ttctgtggct
     5941 tgagacctca tttgcactca acatccgcga acagtcgatt atgaacgtca gcttttgtgg
     6001 tgataaagcc ggggcgacgc tgtttccagc acatatctac accgataaca acggtgagtt
     6061 aatgacgctg atgcaacggg aaatggcaga cgacaaccgt catctgcgca gtatggaagc
     6121 ctttatcaac cacgtacaag gtaagcccgt gatgatagcc gacgccgagc aggggtacat
     6181 catccagcaa ctggtggcgg cgttgtatca atccgcagaa acagggacgc gtgtggaatt
     6241 atgaccaggc cagtaacgtt aacggaaccc catttcagcc agcataccct gaacaagtat
     6301 gcttcgctga tggcgcaggg gaacggctat cttgggcttc gtgccagcca tgaagaagat
     6361 tacacgcgcc agacccgcgg catgtatctg gcagggcttt atcatcgagc tgggaaaggt
     6421 gaaatcaacg aactggtgaa cctgcctgat gtcgtgggga tggagattac cttgaatggt
     6481 gaactttttg cgctatcccg cgaaacgtgg cagcgcgagc ttgacttcgc cagtggagaa
     6541 ttacgtcgca atgtcgtctg gtcttcagcc agcggcgcac gttatgccat tgccagccgt
     6601 cgctttgttt cggcagaaca actgccgctg atggcgctgg aaatcagcat tacgccgctg
     6661 gacgctgacg cgtcagtgct gatttcaaca ggcattgacg ccacgcaaac caaccacgga
     6721 cgacaacatc tcgacgaaac ccaggtgcgg gtgtttggtc agcatttgat gcaggggatc
     6781 tataccactc aggatggacg cagtgatgtg gccatcagct gttgctgcca ggtgagcggt
     6841 gacgtgcagc aatgctatac cgccaaagag cgccgcttgc tgcaacatac cagtgcacaa
     6901 ctacctgcgg gcaaaacgct gacgctgcaa aaacgggtgt ggatcgactg gcgggacgac
     6961 agacaagccg ctttagacga atggggcagt gcgtcgcttc gtcagcttga aatgtgcgcg
     7021 cagcagagtt acgaccaact tcttgcagca tcaacagaaa actggcgtca atggtggcag
     7081 aaacgtcgta tcacggtaaa cggcggcgat gcgcacgatc agcaagcgtt agattatgcg
     7141 ctttatcacc tgcgcatcat gacgcctgcc cacgatgagc gcagcagcat tgcggcgaaa
     7201 ggtttaaccg gcgaaggcta caaaggccac gttttctggg atacagaagt atttttgcta
     7261 ccgttccatc tgtttagcga tccgacggtt gcccgaagtt tactgcgtta tcgctggcac
     7321 aacttgcctg gcgcgcagga gaaagcgcgc gtagcggcga agaagagacg ccggaatttg
     7381 ctgccattaa cattcgtacc gggttgcggc aaaaagtggc ctctgcgcag gcggaacatc
     7441 atctggtggc cgatatcgcc tgggcggtta ttcaatactg gcggaccacg ggggatgaaa
     7501 gtttcattgc tcatgaaggc atggcgctac ttctggagac ggcaaagttc tggattagcc
     7561 gcgcggtgag ggttaacgac cgtctggaaa ttcatgatgt tattgggcca gatgaatata
     7621 ccgaacatgt caataataac gccttcacca gctatatggc gtattacaac gtccagcagg
     7681 cgctgaatat cgctcgtcag tttggctgta gcgacgacgc gtttattcat cgtgcagaaa
     7741 tgtacctcaa agaactgctg ctgccagaaa ttcagcccga cggcgttttg ccgcaggatg
     7801 attcgtttat ggctaagccg gtgattaatc tggcgaaata caaagcggcg gcggggaagc
     7861 aaaccattct gctggattat tcacgcgcag aagtgaacga gatgcagatc ctcaaacaag
     7921 ctgatgtggt gatgctcaat tacatgctgc cggagcagtt ctcagcggca tcgtgtcttg
     7981 ccaatctgca attttatgaa ccgcgcacta ttcacgactc gtcattaagt aaagcaatcc
     8041 acggcattgt tgccgcacgc tgtggcctgc tgacccaaag ttatcagttc tggcgcgagg
     8101 ggactgaaat cgatcttggt gctgatccac atagctgtga tgacggtatc cacgctgccg
     8161 caactggcgc tatctggctg ggggcgattc agggttttgc cggggtgagc gtgcgtgacg
     8221 gtgaattaca tctcaatccg gcgttacctg agcagtggca acagttgtct ttccctctgt
     8281 tctggcaggg ctgcgaatta caggtcactc tcgacgcgca gcgtattgcg attcgaactt
     8341 ctgcgcccgt ttcactgcgc ttgaacggtc agcttattta cgtcgctgaa gaatctgttt
     8401 tctgtttggg ggattttatt ttgcccttca atgggatcgc taccacgcat caggagggtg
     8461 aatgaaactg caaggggtaa ttttcgatct ggatggagta atcacagata gcgcacatct
     8521 gcatttccag gcgtggcagc agattgccgc tgaaattggc atcagcattg atgcgcagtt
     8581 taacgaattc ctaaaaggga tcagccgcga tgagtccctg cggcgaattc tgcagcacgg
     8641 gggtaaagag ggcgatttta acccacagga acgggcgcaa ctggcgtatc gcaaaaatct
     8701 gctttacgtc cactcactgc gcgaactgac ggttaatgct gtgctgcctg gcattcacaa
     8761 tttgctggta gatctccgag cacagcagat tccggttggg ctggcttctg tctccctgaa
     8821 tgcgccgacg attttagcag cgctggagct gcgtgagttt ttcaccttct gcgcggacgc
     8881 ttcccaactt aaaaactcga aaccggaccc ggaaatcttt ctcgccgcct gtgccggact
     8941 gggcgtgccg ccgcaggcat gtatcggcat tgaagatgcg caggcgggca ttgacgccat
     9001 taacgccagc ggtatgcgct cggtggggat cggcgcaggc ttaaccgggg cgcaattatt
     9061 gttgccctca acggaatcac tcacctggcc gcggttatcg gccttctggc aaaacgtata
     9121 gcaaaggaat caacatggct cagctttcgt tacaacatat tcaaaagatc tacgataacc
     9181 aggtgcatgt ggtgaaggac ttcaacctgg agattgccga taaagagttc atcgtgtttg
     9241 tcggtccgtc gggctgcggt aaatcgacca ccctgcgtat gattgccggg cttgaggaga
     9301 tcagcggtgg tgatctgttg atcgacggca aacgaatgaa tgacgttcca gccaaagcac
     9361 gcaatatcgc gatggtgttc cagaactacg cgctgtatcc gcatatgacg gtctacgaca
     9421 acatggcgtt tggcctgaag atgcaaaaaa tcgccaaaga ggtgattgat gagcgggtga
     9481 actgggcggc gcaaattctc ggcctgcgtg agtacctgaa acgcaagccg ggcgcgcttt
     9541 ccggcgggca acgtcagcga gtggcgcttg ggcgggcgat tgtgcgcgaa gcgggcgtgt
     9601 ttttaatgga tgagccgctc tctaacctag atgccaagct gcgcgtgcaa atgcgcgcag
     9661 agatcagcaa gctgcatcag aaactgaaca ccaccatgat ctacgtgacc cacgatcaga
     9721 ccgaagcgat gaccatggcg acgcggattg tcattatgaa ggatgggatt gttcagcagg
     9781 tcggtgcgcc gaaaacggtt tataaccaac ctgcgaatat gtttgttgcc ggatttattg
     9841 gatcaccagc gatgaatttt attcgcggca cgatcgatgg cgataaattc gttacggaaa
     9901 cgcttaaatt aaccataccc gaagagaaat tagcggttct gaaaacacag gaaagtttgc
     9961 ataagcccat cgtaatggga atacgcccgg aagatattca tccggacgcg caagaggaaa
    10021 ataacatttc cgctaaaatt agcgtggcgg aattaaccgg tgcggaattt atgctctaca
    10081 ccacggttgg ggggcacgag ttagtggtcc gtgctggtgc gttaaatgat tatcatgcag
    10141 gagaaaatat cactattcat tttgatatga ctaaatgtca tttctttgat gcagaaacgg
    10201 aaatagcaat ttgctaaata cagggggaag gcattctccc aggataatac aaggaacaat
    10261 aatgaaaaag ttattaccct gtactgcact ggtgatgtgt gcgggaatgg cctgcgcaca
    10321 ggccgaggaa aagaacgact ggcactttaa tatcggcgcg atgtacgaaa tagaaaacgt
    10381 cgagggttat ggcgaagata tggatgggct ggcggagcct tctgtctatt ttaatgccgc
    10441 caacgggccg tggagaattt ctctggccta ttatcaggaa gggccggtag attatagcgc
    10501 gggtaaacgt ggaacgtggt ttgatcgccc ggagctggag gtacattatc agttcctcga
    10561 aagcgatgat ttcagtttcg gtctgactgg tggtttccgt aattatggtt atcactacgt
    10621 tgatgaaccg ggtaaagaca cggcgaatat gcagcgctgg aaaattgcgc cagactggga
    10681 tgtgaaactg actgacgatt tacgtttcaa cggttggctg tcgatgtata aatttgccaa
    10741 cgatctgaat acgaccggtt atgctgatac ccgtgtcgaa acggaaacag gtctgcaata
    10801 taccttcaac gaaacgcttg ccttgcgagt gaactattat ctcgagcgtg gtttcaatat
    10861 ggatgatagc cgcaataacg gtgagttttc cacgcaagaa attcgcgcct atttgccgct
    10921 gacgctcggc aaccactcgg tgactccgta tacgcgcatt gggctggatc gctggagtaa
    10981 ctgggactgg caggatgata ttgaacgtga aggccatgat tttaaccgtg tgggtttatt
    11041 ttacggttat gatttccaga atggtctttc cgtttcgctg gaatacgcgt ttgagtggca
    11101 ggatcacgac gaaggcgaca gcgataaatt ccattatgcg ggtgttgggg tgaattactc
    11161 gttctgataa tgtgctaaat tgccggatgc ggggcgagcg ccaaatccgg catacattta
    11221 tttcaacgac atatgcgtag caacggtaat attttgtggt gacggttttc cggcaattaa
    11281 ctggaataat aactcgcagc ttcgttgacc taactcctgc gtcggaacat cgatgccgcc
    11341 tggtgcaggc gttaatataa acgacagcgt ttcattacta taacccacca ccgctaactg
    11401 ctgcggaata gcaatgtttt tctctgccgc cgcacgataa atgctcatta atttcaggct
    11461 gtcagtggca aatacggctt ctggcaacgg cgactggctt aataattgcc gtgcggcttg
    11521 caaggctgtt tcatgggtat aaccgccgtc aacaatccat tcatcacgca ctgcaatatt
    11581 atgcgcagcc aggctttgct tataaccatt tacccgatcc actgaaacat gtacatcaag
    11641 cggcgcatgc aggcaggcta tattcttatg cccgctttca attaacgcat tggtcagcgc
    11701 aatactgtcg ccataattat cggtatcgac ggaataaaca tggctgtatt gaccttcaac
    11761 tttaccgatc accaccaccg ggatctcgta tttgtcgagt tgtgcaaaga aggattcatc
    11821 ggcaggcgaa cttagcataa taatgccttt aatcattttc tgcttaattt tgctttcgca
    11881 tttttgcaga tcttctgccg gattgtgcga cgtttgtaat atcacgtcga aaccttcttc
    11941 ttctgctttg gcggtgatgg catgcaaaac ttccgagaaa aatggattac ccgccgtagt
    12001 tttggtcgaa cgggtagaaa tcaccataat ggcatcaaaa cccgaagagg tcagcgcgcg
    12061 ggccagcttg tttggttgat actgtaattc ttcaatggcc cgtaacactt tttcgcgcgc
    12121 ttccggggag atattggttt gcttattcag cacgcgtgat acggtggatt ttgatacgcc
    12181 tgcaaccctg gcaatatcat aaatagtagg ggacataggt caggaactcc gtccgaacgt
    12241 gaatgctgca catcttatgg agttcgtccg tcgatgacaa cacgggtagt tgataatcaa
    12301 tggcctggcc cccacattca tatccttacg aacgattttt tttctggtct tcagagcagc
    12361 acaggacagc aatgaagcga cttaaaaatg aactcaatgc gctggtgaat cggggtgtcg
    12421 acagacatct gcgcctcgct gtaaccggac ttagccgcag cggcaaaaca gcgtttatca
    12481 ccgcgatggt taatcagttg ctcagtattc atgccggagc acgtttgccg ctattaagtg
    12541 cggtgcgtga agagcgcctg ctgggcgtaa aacgcattcc ccagcgtgac tttggcatta
    12601 cgcgcttcac ctatgatgaa gggttggcgc agttatatgg cgatcctccc gcctggccga
    12661 cgcctacgcg cggcgtcagt gaaattcgcc tggcgttacg ttttaaatcg aacgattcgc
    12721 tgctgcgcca ctttaaggac acctccacgc tgtatctgga gattgtggat tatcccggcg
    12781 aatggctgct cgacctgccg atgctggcac aggactattt tagctggtcg cgccagatga
    12841 cgggcttact caatggtcag cgcggcgaat ggtcggcgaa atggcgaatg atgtgtgaag
    12901 gtctggaccc gttagcgcct gccgacgaaa atcgcctggc ggatattgcc gctgcatgga
    12961 ccgattatct ccaccactgc aaacagcagg ggctgcactt tattcagcca ggacgctttg
    13021 ttttgcctgg cgatatggca ggagcgcccg cgctgcaatt cttcccatgg ccagatgtcg
    13081 atgcctgggg cgagtccaaa ctggcgcagg ccgataaaca caccaacgcc ggaatgttgc
    13141 gcgagcgttt taattattac tgcgagaaag tggtgaaggg gttctataag aatcacttcc
    13201 tgcgttttga ccgccagatt gtgctggtgg actgcctgca accgctcaac agtggaccac
    13261 aggcatttaa tgatatgcgt ctggcgctga cgcagctgat gcaaagtttc cactacgggc
    13321 agcgtaccct gttccggcgt ttgttttcgc cggttatcga taagctattg tttgctgcca
    13381 ctaaagcgga ccatgtgacc atcgatcagc acgctaatat ggtttcattg ctgcaacaac
    13441 tgattcagga tgcctggcaa aatgcggcgt tcgaagggat cagtatggac tgcctggggc
    13501 tggcgtctgt tcaggcgacc accagcggaa ttattgatgt taacggtgag aaaatcccgg
    13561 cgctgcgcgg taatcgactc agcgatggcg caccgctcac tgtttatcct ggcgaagttc
    13621 ccgcacgttt gccaggtcag gcgttctggg ataagcaagg cttccagttt gaggcgtttc
    13681 gtccgcaggt gattgatgtc gacaaaccgc taccgcatat tcgtcttgat gctgcgctgg
    13741 aatttttaat aggagataaa ttgcgatgac cgaaccgtta aaaccacgta ttgatttcga
    13801 cggtccactg gaggtcgatc agaatccaaa attcagggcg cagcagacct ttgacgaaaa
    13861 tcaggcgcaa aattttgccc cagccacgct cgatgaagcg caggaagaag aggggcaagt
    13921 tgaagcggtg atggacgcag cgttgcgtcc gaaacgcagc ctgtggcgca aaatggtgat
    13981 gggcgggctg gctctgtttg gcgcaagcgt tgtcgggcag ggcgtgcagt ggacaatgaa
    14041 cgcctggcaa acccaggact gggtggcgct gggtggatgt gccgctgggg cattgattat
    14101 cggcgctggc gtaggttctg tggtaacaga gtggcggcgc ttatggcgct tgcgacagcg
    14161 cgcccatgaa cgcgacgaag cgcgtgattt attgcacagc catggcaccg gcaaaggccg
    14221 cgcattttgc gaaaaactgg cgcagcaggc gggcatcgat cagtcacatc cggcgctgca
    14281 acgctggtat gcctcaatcc atgaaacgca aaacgaccgt gaagtggtca gtttgtatgc
    14341 gcatttggtc cagccagttt tagatgccca ggcgcggcgc gaaatcagcc gttcggcagc
    14401 ggaatcaacg ttaatgattg ccgttagccc actggcgctg gtggatatgg cgtttatcgc
    14461 ctggcgcaat ctgcgtttga ttaatcgcat cgccacgctg tatggcattg aactgggata
    14521 ttacagccgt ttgcgcctgt ttaagctggt attgctgaat attgcttttg ccggagccag
    14581 cgaactggtg cgcgaagtgg ggatggactg gatgtcgcaa gatctcgctg cgcgtttgtc
    14641 tacccgggca gctcagggga ttggtgctgg acttctgacg gcacgactgg ggattaaagc
    14701 tatggagctt tgccgcccgc tgccgtggat tgacgatgac aaacctcgcc tcggggattt
    14761 ccgtcgtcag cttatcggtc aggtgaaaga gacgctgcaa aaaggcaaaa cgcccagcga
    14821 aaaataatgc aatatcgggt gctgaccgga tatctttacg ccgaagtgcc cgtttttccg
    14881 tctttgtgtc aatgattgtt gacagaaacc ttcctgctat ccaaatagtg tcatatcatc
    14941 atattaattg ttcttttttc aggtgaaggt tcccatgcgt ctggaagtct tttgtgaaga
    15001 ccgactcggt ctgacccgcg aattactcga tctactcgtg ctaagaggca ttgatttacg
    15061 cggtattgag attgacccca ttgggcgaat ctacctcaat tttgctgaac tggagtttga
    15121 gagtttcagc actctgatgg ccgaaatacg ccgtattgca ggtgttaccg atgtgcgtac
    15181 tgtcccgtgg atgccttccg aacgtgagca tctggcgttg agcgcgttac tggaggcgtt
    15241 gcctgaacct gtgctctctg tcgatatgaa aagcaaagtg gatatggcga acccggcgag
    15301 ctgtcagctt tttgggcaaa aattggatcg cctgcgcaac cataccgccg cgcaattgat
    15361 taacggcttt aattttttac gctggctgga aagcgaaccg caagattcgc ataatgagca
    15421 tgtcgttatt aatgggcaga atttcctgat ggagattacg cctgtttatc ttcaggatga
    15481 aaatgatcaa cacgtcctga ccggtgcggt ggtgatgctg cgatcaacga ttcgtatggg
    15541 ccgtcagttg caaaatgtcg ccgcccagga tgtcagcgcc ttcagtcaaa ttgtcgctgt
    15601 cagcccgaaa atgaagcatg ttgtcgaaca ggcgcagaaa ctggcgatgc taagcgcgcc
    15661 gctgctgatt acgggtgata ccggcaccgg aaaagatctc tttgcgtatg cctgtcatca
    15721 ggcaagcccc agagcgagca aaccttacct ggcgctgaac tgtgcgtcta taccggaaga
    15781 tgcggtcgag agtgaactgt ttggtcatgc tccggaaggg aagaaagggt tctttgagca
    15841 ggcgaacggt ggttcggtgc tgttggatga aataggtgag atgtcaccac ggatgcaggc
    15901 gaaattactg cgtttcctta atgatggcac cttccgtcgg gttggcgaag accatgaggt
    15961 gcatgtcgat gtgcgggtga tttgtgctac gcagaagaat ctggtcgaac tggtgcaaaa
    16021 aggcgtgttc cgtgaagatc tctattatcg tctgaacgtg ttgacgctca atctgccgcc
    16081 gctacgtgac tgtccgcagg acatcatgcc gttaaccgag ctgttcgtcg cccgctttgc
    16141 cgacgagcag ggcgtgccgc gtccgaaact ggccgctgat ctgaatactg tacttacgcg
    16201 ttatgcgtgg ccgggaaatg tgcggcagtt aaagaacgct atctatcgcg cactgacaca
    16261 actggacggt tatgagctgc gtccacagga tattttgttg ccggattatg acgccgcaac
    16321 ggtagccgtg ggcgaagatg cgatggaagg ttcgctggac gaaatcacca gccgttttga
    16381 acgctcggta ttaacccagc tttatcgcaa ttatcccagc acgcgcaaac tggcaaaacg
    16441 tctcggcgtt tcacataccg cgattgccaa taagttgcgg gaatatggtc tgagtcagaa
    16501 gaagaacgaa gagtaagcgt gaatatgcct gatggtgcaa caccatcagg catattaact
    16561 tatgctttca gtacagccag agctgcttcg taatccggct cggtggtgat ttcatccacc
    16621 agctggctga aaatcacatt gtcattttca tcaataacca caacggcacg cgctgccaga
    16681 cctttcagtg ggccatcagc aattgccaca ccgtaggctt gcaggaattc agcgttacgg
    16741 aaagtggaga gggtgataac gttgttcaga ccttctgcgc cgcagaaacg agactgggcg
    16801 aacggcagat cggcagagat acacagcaca acggtgttgt cgatctcggt tgccagttgg
    16861 ttaaacttac gtactgatgc ggcgcaaaca ccggtatcaa tactcgggaa aatgttcagc
    16921 actttgcgtt tacccgcaaa ctgaccgagg gtgacgtcag acagatcttt tgccacgaga
    16981 gtaaaagtct gcgctttgct acccgcctgc gggatggaat tggcgactgt aaccgggttg
    17041 ccctggaaat gaacggtttg tgacatgatt atctttcctg tttacatata gttaacgtca
    17101 cacctagttt atgctaactg tcaataacac agcaaacgct atttgcgctt aatccgcaga
    17161 ccaccgcgac aacaaggagt aaagatgaga accgttaagg tatttgagga ggcctggccc
    17221 ttacataccc cgtttgtgat tgctcgggga agtcgcagtg aagcgcgcgt ggtggtggtt
    17281 gaactggaag aagagggtat taaaggcacc ggcgaatgca cgccgtatcc gcgttatggg
    17341 gaaagtgatg cctcggtaat ggcgcaaatt atgagcgtcg tgccgcaatt agagaaaggg
    17401 ctgacacggg aggagttgca aaaaatcctc cctgccggcg cagcacgtaa tgcgctggat
    17461 tgtgcattgt gggatctggc ggcgcgaaaa cagcagcaat cgctggctga tttgatcggc
    17521 ataacgcttc ccgagacagt caccacggca cagacgatag tcatcggtac gcctgatcag
    17581 atggccaata gtgcatcaac actctggcag gcaggcgcga aattactgaa agtgaagctg
    17641 gataaccatc ttatcagtga gcggatggtg gcaattcgca cagctgtgcc cgatgcgacg
    17701 ctgatcgttg atgcaaatga atcctggcgt gcagaagggt tggcggcgcg ttgccagcta
    17761 ttggcagatt taggcgttgc gatgcttgaa caaccgcttc ctgcgcagga cgatgcggcg
    17821 ctggagaatt ttattcatcc gttaccgatt tgtgctgatg aaagttgtca tactcgtagc
    17881 aatctgaagg cgctgaaagg gcgctatgag atggttaaca ttaagcttga taaaaccggt
    17941 ggtctgacgg aagcgctggc gctggcgact gaagcgcgtg cacaaggttt tcgtctgatg
    18001 ctgggctgca tgttgtgtac ctctcgtgcc attagcgccg ctttaccgct ggtgccgcag
    18061 gtcagtttcg ccgatcttga cggaccgacc tggctggcgg tagatgtgga accggcgctt
    18121 cagttcacga cgggcgaatt gcatctttag gatgccagcg cagcaagttt gccatcgcaa
    18181 agagatattt ttcgctggct tcgtcggagg agatgggtgg aaactctgcg gtgatgcaat
    18241 gcaagttgag atctgcacac caactgccga aagagccagg cgtttcataa ccgacgctgg
    18301 ttaccagagg caattcaaac gcctgggcca gccattcacc taattcgctg tgtctgggat
    18361 cttcaataca ggccagcgga tcgtggaatg agaccaccca ggcaggttga atgcggtgta
    18421 taagctggca cagcgcctgg gtttcaggtt cggagccagg tttgtcacct gtcagtaaaa
    18481 cgacatcacg ttcttcagcg gcgctgttcc agcgataaac tgtttcacct tccttccagt
    18541 tcgctgccgg aaagtttcgg tttaaatcca caccattagc attggcccgt aaccctaact
    18601 ggcagccgtc aggattcaca cacagcacca catgatggcg gcgcaaagaa ggtgtcaaag
    18661 tccgcagagc acaggagagc gtgacgatcg aagagttttc atcaccgtga gtgccagcca
    18721 gaatcaaacc actttcgtga ctggctgcag gggccggaaa ccagattaac ggcgcgccca
    18781 ataatgaacg tccgtaatgt tctgttccgg gcggaaatgc gccgcgttcg gcgcgtgggc
    18841 gagttacggt catgatcgtc tctggttcag gaagtattac aggcagtgtt gtgcaaattt
    18901 tgcggattat caaatccttt tcttcaacgc tatgtcatcg atcttgtatc acttttttgc
    18961 cggaagttgt ttgtattttc attgatgtgc agatattaga attgtcgtaa tgttatatac
    19021 atggcgtatt ttattcttac tggttctaca catgttaatc gcatctgatt gatcagttca
    19081 ccttaataag tttaataaat cgtactgcac atagtacagt acgatggtag ctatataatt
    19141 gatttacatg aagaagggct tatcaccatc agtcccgggt tgattgatgc ctatattctc
    19201 accaatgtgt ttctcaggtt tatcgatgat atctgtgatt aatgctgcga cactcttacg
    19261 tgatacgatc gtacctttga atggttcatt gcgtgaggta agctcataat caataatatc
    19321 ttcatcagtc agccaggcag ggcgaagaat tgtatattcc agtccagaag cctcaattgc
    19381 atccgcagcc ctgcggaatg gttttagggg ttcaccaata actgcattat tccattcgcc
    19441 aaattttcct ggaacttcat cataaatacc gagggaaaga acaaagatca gacgtttaac
    19501 atcacaggct ttcatcgcag cgatcactga atttgcctga atatctaaat cttcccccgt
    19561 aaggttggca tacacaacat cctgtccctg catggcctgc ttcagtgctg catgattcag
    19621 tacatccccc ataataatct tgctattagt gggatagggt ttgtggattt ttgctggctg
    19681 acgggcaaat aaagtttgtt taatggtttg tttatctgcc agttgattaa tcacatggcg
    19741 ggctatctga ccgccagcac caagaatcag tatatttttc atgaaacttt tcccagatta
    19801 ttggcagcaa tatgccaggc catatcacta ttacggattt gaatatgttt cattacagag
    19861 tttcctcaaa gaacggagct aatactgaaa ttgcttcagc cacatagact ttgccgtcat
    19921 aaagatccat atggtttgcg ccttcaacaa tgtgatagcg tttatccttg cttgatgctc
    19981 gatcgtacag gtcgtcactc atccatttgc tcccagcctg gctgcccgcc acaatctgta
    20041 tcggctgagt caggtacact tccgccatat ggtaagcatc ataggtaata atctggttaa
    20101 ggctgcgcaa agtagcgtaa cccggtgctg ttggatactg cgcgcgaggg gtgtggtaat
    20161 actcccaggc ctgacgcagt tcttcgttcg gcgcatcgga ctccttcatt ggtgccagtg
    20221 gcatagtggc gtattctccg ctgctaatat cgctggttcg ggcgtctgaa cccgcgtcaa
    20281 cgtatggtag ggcatcaata gatttcacat tgttttccca accattacgg aacatcgaac
    20341 caatattgac cgcactaaca gtaccgatgg ccttgatgcg gcgatcctga attgcagcat
    20401 tggctgtata tcctgctccg gcacaaattc ccatcgcacc aattcgggta ttgtcgacat
    20461 aagaaagcgt tgtcaggtaa tcaatcacgg cactgacgtc ttcagtacga atgtatgggt
    20521 tttctaactg acgcggctcg ccgccgcttt caccttgata agatgcgtca taagcaatag
    20581 tgacaaatcc cttttccgcc agtttttcgg cataggttcc ggccgtttgc tctttaacgc
    20641 ccccacctgg gtgagatacc acaattgccg gatactgacg ggtttcatca aattttggag
    20701 ggaaatagat cactgcagac aaagagatgg ttggattatt gctgttagtg aagctgactt
    20761 tattattcat cattccgttc ctcatggagt tgtcggttcg ttttaacggt tggtgatatc
    20821 actatagata ttgatcatta agttgattag acccaaaatc atgattagac ctatcattta
    20881 aatgattaat agattgtatg gagatactga atgaagcgtg aagaaatcgc tgatctgatg
    20941 gcgtttgtcg ttgttgcaga ggagcgtagc ttcactcgcg cagcagcccg cctgagcatg
    21001 gcgcagtcag ctttaagcca gatagtgcgc cgtatagaag aacgattggg attgcggctt
    21061 ctgacgcgaa ccacgcgcag cgttgtaccc actgaagcgg gcgagcatct tttgtctgtt
    21121 cttggcccta tgttgcatga catagattct gccctgacat ccctgagcga tctgcagaac
    21181 cgcccatccg ggacaatacg tattactact gtagaacatg cagcaaaaac gatattgtta
    21241 ccagctatgc gcacattcct gaaatcgcat cctgaaattg atattcagct taccattgat
    21301 tatggtttga ccgatgtcgt ttctgaacgt tttgatgcag gcgtccgtct gggtggggag
    21361 atggataaag atatgatcgc cattcgaatc gggccagaca taccaatggc tattgttggc
    21421 tcaccggatt atttttctcg tcgaagtgct ccaacgtcag tgtcacaatt aatagatcat
    21481 caggcaatta atttgtatct tcccacatcg ggtacagcaa atcgctggag attaatacgc
    21541 ggtggacgtg aagttcgtgt tcgcatggaa ggtcagcttt tactgaatac gatagacctg
    21601 atcattgatg ctgcaattga tgggcatgga ttggcgtatc taccttatga tcaggttgag
    21661 cgggctatta aagaaaaaaa actgatacgt gttttggata aattcacacc agatttaccc
    21721 ggttatcacc tgtactatcc acaccgtcga catgctggct cggcattctc attatttata
    21781 gataggctga agtataaagg tgctgtttag cactacttgc tgatacatta atttaattct
    21841 tctcttaacg tattctcagt tcctttcaac gttttggtta ttttttattc ttcgcacaat
    21901 ggcgacagat gctgattatg ataccgaaaa cgggtttgaa cgtgcgaagc ccgaacgtag
    21961 tgttcggagt tctatgtgct ttaccgcatt ttggagacta ttatttacac taaatctgat
    22021 ttgatatatt gatacttaaa acatttgatg cttcctttgt cacttttttg atggaagttg
    22081 tttgcatttc tttaaggcga aacaaataat tacgcatcaa ttttaatgtc ggttagaggg
    22141 aaacttatga agcactctgt ttcagtcacg tgttgtgcgc tgttggtcag cagcatttct
    22201 ctttcgtatg ctgctgaagt tccgagcggc acagtactgg cagagaagca ggagctggtg
    22261 cgccacatta aagatgagcc agcgtcgctg gatcccgcta aagccgtggg cctgccggag
    22321 attcaggtca ttcgcgatct gtttgaagga ctggtgaatc agaacgagaa aggggagatt
    22381 gtccccggcg ttgcgactca gtggaaaagt aatgacaacc gtatctggac ttttaccctg
    22441 cgcgataacg caaaatgggc ggatggcaca ccggtaacgg cgcaagattt tgtctacagc
    22501 tggcaacgtc tggtggaccc gaaaacgctt tcgccatttg catggtttgc cgcgctggcg
    22561 ggaatcaaca acgcacaggc gattattgat ggtaaagcca cgcctgacca gcttggcgtc
    22621 accgcagttg atgcccatac tttgaaaatc cagcttgata aaccattgcc gtggtttgtg
    22681 aatttaaccg ctaactttgc cttcttcccg gtgcaaaaag ccaacgtaga aagcagtaaa
    22741 gagtggacga aacccggaaa tctgatcggc aatggcgctt atgttcttaa agagcgcgta
    22801 gtcaatgaaa aactggtcgt agtgccgaat acccattatt gggataacgc caaaacggta
    22861 ctgcaaaaag tgaccttcct gccaattaat caggaatccg cagccactaa gcgttatctc
    22921 gcgggggata ttgatatcac cgaatccttc ccgaaaaata tgtatcagaa gctgttgaag
    22981 gatattccgg ggcaggttta tacgccgccg cagctcggga cctattatta tgcgtttaac
    23041 acgcaaaaag ggccgacggc agatcagcgc gttcgcctgg cattaagtat gacgatagat
    23101 cgccgcctga tgaccgaaaa agtgttgggg acgggcgaaa agccagcgtg gcattttaca
    23161 ccagatgtta ccgcgggatt tacgccggaa ccttcgccgt ttgaacaaat gagtcaggaa
    23221 gaactgaatg cgcaggcaaa aactttgttg agcgctgctg gttatggtcc gcaaaaaccg
    23281 ttgaagctga cgcttttgta taacacttca gaaaaccatc aaaaaattgc gattgctgta
    23341 gcatcgatgt ggaaaaagaa cctgggcgta gatgttaaat tgcaaaacca ggaatggaaa
    23401 acctatatcg atagccgtaa taccggcaat tttgatgtta tccgcgcctc gtgggtaggg
    23461 gattataatg aaccctccac tttcctgaca ttattaacgt caacgcattc cgggaatatt
    23521 tcacgcttta acaatccggc atatgacaaa gttctggccc aggcatcgac ggaaaatacc
    23581 gttaaagcgc gtaatgccga ttacaacgcg gcagaaaaaa tcctcatgga gcaagcaccg
    23641 attgcaccaa tttatcaata taccaatgga cgattaatca agccgtggct gaaaggttat
    23701 cccattaata accctgaaga tgtggcgtac agtcggacta tgtatattgt gaagcattaa
    23761 tgtgatggga actggcgtta cccttgtgga taacgccagt gatgtctgat ttagcgaccc
    23821 tggtccggcg gtgtaatgtt atccatatac agcgtctggc tggggaaggc aaagtccgcg
    23881 ccgtgtgact gtacaatatc gataatcttc aaataaacgt cttgctgtgc agcaagccac
    23941 tcagcccata ccgtggtttt ggtaaagcaa taaaccataa tatttaatga cgaatcagca
    24001 aactggttga aataaaccag taaggtttgt cgctggtcga tggccgggtg atttttcagc
    24061 atctcacgta cggcttcgac aataacgccc acttttgccg catcctcata acgtaaacca
    24121 atggtcgtgg taatgcgccg gttggtcatt cgtccaggat tttctacgct gatcgacgaa
    24181 aacagcgagt tcggtacgta caatggacga ttatcaaagg tcttaatttt tgtaattcgc
    24241 cagccaattt ccgttactgt accttcgata tttctgtccg gtgaacggat ccagtcgcca
    24301 atactgaaag gacggtcgaa atagagcata atcccggaaa agaagttact cagaatatct
    24361 ttaccggcca taccgacagc cagaccacca ataccaccaa aggtcaacaa gccagaaagg
    24421 ctcatgccga aatgttcgcc ataaagcaga acaagcacca caataatggt gattttgata
    24481 atgcgcgaca tgatccgcgc actggtgata tcgcgacctt ttttaatctg ctgtttttca
    24541 aactgattaa tcagcagaaa tagcttaatc gtcagaataa ccgcaatcag ggacgtacag
    24601 ataaaatcga taacgcctgg ggtgataaat ttgagtttat agttttctat aacatagtta
    24661 atgatgctac caacagcact aataattatg gtgtagatta aaaattgcac cgcatggaat
    24721 aaaaatcctt ttcttttacg atttccacgg cgaaaccaaa agctcatcag aatcaatgct
    24781 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
    24841 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcgggacta
    24901 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
    24961 taactaatca atgcgttgat tgtaaatcca gctaagaggt gagattttca gagcagacaa
    25021 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
    25081 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa gaaatttctc tttggtttgt
    25141 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gaccatcggc ggcgttatag
    25201 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
    25261 tgatttttgt ttatagcctg gtctttacag tgttgctggc aatcccgttg ggaatttatt
    25321 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
    25381 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
    25441 aggtttaatg accagcaggt cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
    25501 aaggaatgct gctgaaatac cggtgcgtcc gaccgtgccc agaaccacaa tccccgcctg
    25561 taagtgctcc gccaaatcag gaatcacctc ttcgggcaga cctttttcta cgtgcgtcat
    25621 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttggccacg
    25681 aatggcatcg ttataaacgc tcgggtcaaa ttccggcagt tcaatcgcga tattaattgg
    25741 cgttaccgga taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
    25801 gatcgtctct ttgaccagtt tttcattgag cgcattatgg tacggctctt cactggcgag
    25861 attcaccgcc accagcgcct tacccccttc cggccacggc tggtctttca ccatccacac
    25921 ggggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
    25981 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
    26041 aatgatggct tcgaaaggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
    26101 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
    26161 acgcatagcg gtacgttcgt ccggagagag cagggtggtc atttcgtatg agaagtcata
    26221 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
    26281 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
    26341 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
    26401 tctggtgaat gaaacagtga tgaaccttct gtcagatcaa taaatcagaa aaatttaatg
    26461 atatgacaga aggatagtga gttatgcgga agaatcaggc aacgttacgc gtatgaccag
    26521 caagctgggc cagcgcatcg ttattttcga tagtgatgta tttacctttg actgccagca
    26581 taccgctttt ctggaagcga cccagcagac ggctgatggt ttcaaccgtc aggcccagat
    26641 agttaccgat atcaccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
    26701 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
    26761 tcgacaacag caggatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
    26821 tctgctgacg cagattcggc attttaccag acaaatcgtc cagcgtttcg aacgggattt
    26881 cacataccat cgaggtttcc agcgcctgcg cgaaactcgg gtgatgaccg ctgccgatgg
    26941 catcaaatcc caccagatcg cctgctaaat ggaaaccagt gatttgctcg tcgccttgct
    27001 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
    27061 ctccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
    27121 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
    27181 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
    27241 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
    27301 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
    27361 tctatctatt tgaaaaccct taagttgtta agggtgactt tacataaaag tgtgaacaag
    27421 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
    27481 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
    27541 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
    27601 gccagggcgg cctaattgtt cagccagttg gccataatgc attacctgcc cgcaggggat
    27661 agtgcgcagt gttttccaga cttcgcgctg aaatggtgtc acccccgtgg cagtgggaag
    27721 cgtatcaata atgctaagat taccggcgaa atattcacga agcttgtcgc ttaaaccacc
    27781 tgggttggtg gcagaaatgc gctcatagcc atctttgcga taatggatgt ccagcagctg
    27841 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
    27901 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
    27961 ttctctctta agacaaacgt gggtaaatac ccggacctat cggcaagcca acaagatacc
    28021 acgtcagcaa catcagcagc cataccaccg aaaagattaa tactaacaaa aagtaagtac
    28081 caggtttagc gtctggtttg tcgaagtatt taagcataaa gcgatttttt gatttttctg
    28141 cctgaagaaa tgagggcaag ccatcaaatt ccagtcagcg aaattttacc gtaggctatc
    28201 cgttatttga ttctgtggcg tgatagccgc cagacaccac tgatctcgtc actcattccc
    28261 caatcccggc gagaatgtgg ttatttacag agctaaaaaa tgaataaaaa aatagagcct
    28321 gtcgacatcc gccagactct acagtacaca cagcagtgca tcctcgtctt aaatccggcc
    28381 tggggtcctg gtgaccggtg acagtgttat aacggtttta cccatccgtt gccaattttc
    28441 ttcagtgaca gatttcacga aaatataagt taaatcattt gtcttgtgat tgcataatca
    28501 tgcatcgtta atcgacgagt aaatttaccg ggtgagtagc ccgttcgacg cttaaacata
    28561 ttgccgaacg attcagctga ggcatagccc agacgatggg caattttggt caacgaatat
    28621 ccatcatcaa gaagcgtcac tgcagaaata acgtgcattt gctgaaccca gtggcgaaat
    28681 gttaatcccg tctctttaac aaataagcgc atgagtgtac ggctactgac gttaatttta
    28741 tccgctagat tatccagtgt tggcagatac ccttggttgt ttagcaaatt ctcacaaagc
    28801 tgctgtagcc gctcatcatt tggccaaggg agttcaagtg acattttcac ccccattttt
    28861 atttcctgaa gtatcagtgt ctcgagggct gaaaaacgta actgttgttt atctgtggta
    28921 ctttgcgacg gtggaagttg gttaattgca attagtaatt cccttaacag gggggaaata
    28981 cccgtcatga agcaaacttt tcccagcgtt tcactttccg cttctgaaac cagtacggtt
    29041 tttaaaagta tgttgcctgt cgcaacaaac ttatgcggca tttgtggggg cagccaaagc
    29101 gcgctgctgg catgcactag ccagacgctt tctccagccc atatacgaac gactccttca
    29161 gccgggtaaa gcaactgggc atagagatgt ttatgccagg gaactgtgcc tccattcaga
    29221 tgttccgaat ccagtccgat aatgtgatgc cctggtgatg ctttgggttg cagtgtgatt
    29281 gtcatttaca gtaggtattt tgtcagaaat catcaaataa gccatgttaa attttaaata
    29341 tgacgtatgc aagtttccgg atatatacgg tgtaaagagt gatgataatt tctaaaaaac
    29401 agcttattgg ggtggtagcg atcggtattc ttttggctgg cgttgtcttt tttatctggt
    29461 gggtaagcaa aggaagattt atacagacga cggatgatgc ttacattggt ggtaatatca
    29521 ccacggtggc aagtaaagtt tctggttata tttctgctat agaggtgcgc gataatcaga
    29581 gtgtaaaaaa aggcgatatt atcctgcgtc tcgatgatcg ggattattgt gcaaatgtcg
    29641 ccaggcttga ggcaaaaata aaaagtagca aagccaatct cgaaagcatt caagccacta
    29701 ttgcgatgca gcagtcgata attcaaagtg ccagtgaaac atggcaggca gtaaaacatg
    29761 aggagcaaaa gcgcctgcga gatactgaac gttatgaaaa actcgcacaa tcagcggcaa
    29821 tatcgcaaca aattattgat aacgccaggt ttgactacca gcaggtcgca gcaaaggagc
    29881 gcaaagccgc gaacgatttc ctggtggaga agcaacggct ggcagtttta tctgctcagg
    29941 aagaaaacgt acgtgcaagt atcgaagaag ttcaggccgc tttgactcag gcactactgg
    30001 atctggaata tacccttgtt cgtgcgccga ttgatggcat tgtcgcaaac cgcagcgccc
    30061 atacaggcag ttgggtggaa ggcggcacat cgttagtttc actagtacca gtcagtgagt
    30121 tgtgggttga tgcaaattat aaagaaaacc aaatagcggg aatgaagcca ggaatgaagg
    30181 ctgagatccg tgctgatatt ctcaaagggg aagtgtttca cggtcacatt gaaagccttt
    30241 ccccagcgac aggagccagt ttcagcttga ttccgataga aaatgccact ggcaatttta
    30301 ccaaaattgt tcagcgtgtc ccggtacgga ttgtatttga cgacgcaaag gaattaaagc
    30361 aacttcttcg cccgggactg tccgtcaccg tcagtgttga tgagagatga tgtctatgcg
    30421 taaacatatt gctttcgcca gtatgtgtat tggattattt attgctcagc ttgatattca
    30481 aattgtatcg tcatccctaa atgaaatagg tggtggtctg tccgcaggta aagacgaaat
    30541 ggcctagctt cagaccagct atttaattgc cgagattatt gttattccgc tctctgggtg
    30601 gctgtcacgg gtattttcca ctcgttggct ttttactcta tccgcgggaa tatttacttt
    30661 aatgagtatc gcttgtggac ttgcatggaa tattcagatc atgatttttt ttcgtgcttt
    30721 acagggagtt gctggcgcat ccatgatccc tttagttttc acgacggcgt ttatttatta
    30781 ccagggtaaa gagttggggt tggctgctgc ggtagtcagt gcgctggcct cactgtcacc
    30841 gacgctaggg ccaactctgg ggggctggat cactgataac ctcgactggc gatggctatt
    30901 ttatatcaat atattaccgg gcatatattt agttttgagt attccttttc tggttaattt
    30961 tgataaacct gatctctctt tactgaaagt tgctgattat ccgagtatta tcctgttggc
    31021 catgacactg ggatgtctgg aatataccct tgaagaagga gcgcgctggg gatggctgga
    31081 tgataatact attttgctga catcagttct ggcattggta agttttattt tatttgctgc
    31141 gcgtacactt acaatatcca atccgataat ggatcttcat gc
//
LOCUS       DAZGJT010000050        31074 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZGJT010000050 DAZGJT010000000
VERSION     DAZGJT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.050"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..1202)
                     /locus_tag="ACHKFK_002526"
     CDS             complement(87..1202)
                     /locus_tag="ACHKFK_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO9052840.1"
                     /translation="MGVNSDVYAADVNIDILSATVKDKRIEGVSVTLQRNGAQSVSGT
                     TNASGSVNLGSTFADDQDALLIVKKEGYSNLVVKCSCAGMTYAISPAMTSLDGMRVVL
                     SWGEKPFDLDSHLIFSGGHIYFDSKEGTDANLDVDDTDSYGPETVTISKKHFGESYIY
                     AVQDYSNKGLPNSNYLSASKAKVFVYVGSSLVRSYSVPAGKRGNIWTVFKLNPNGEFE
                     DINSVTSANFNDTTLDVRDLATVIMPATDSSAPASPAMQNSGDTQLARKYNREGEAVY
                     KTGQLEQAIQLFQQATELDGNYGQAFSNLGLAYQKNGNIAEAIWANRKAISLASGANA
                     ATTRANSYYNIAKIYETAGQDADALQHYQLALHDAIL"
     gene            complement(1442..2311)
                     /gene="sucD"
                     /locus_tag="ACHKFK_002527"
     CDS             complement(1442..2311)
                     /gene="sucD"
                     /locus_tag="ACHKFK_002527"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO9052841.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(2311..3477)
                     /gene="sucC"
                     /locus_tag="ACHKFK_002528"
     CDS             complement(2311..3477)
                     /gene="sucC"
                     /locus_tag="ACHKFK_002528"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO9052842.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(3571..4788)
                     /gene="odhB"
                     /locus_tag="ACHKFK_002529"
     CDS             complement(3571..4788)
                     /gene="odhB"
                     /locus_tag="ACHKFK_002529"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO9052843.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAPAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(4803..7604)
                     /gene="sucA"
                     /locus_tag="ACHKFK_002530"
     CDS             complement(4803..7604)
                     /gene="sucA"
                     /locus_tag="ACHKFK_002530"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO9052844.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(8155..8871)
                     /gene="sdhB"
                     /locus_tag="ACHKFK_002531"
     CDS             complement(8155..8871)
                     /gene="sdhB"
                     /locus_tag="ACHKFK_002531"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO9052845.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(8887..10653)
                     /gene="sdhA"
                     /locus_tag="ACHKFK_002532"
     CDS             complement(8887..10653)
                     /gene="sdhA"
                     /locus_tag="ACHKFK_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO9052846.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVIESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(10653..11000)
                     /gene="sdhD"
                     /locus_tag="ACHKFK_002533"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(10653..11000)
                     /gene="sdhD"
                     /locus_tag="ACHKFK_002533"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO9052847.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(10994..11398)
                     /gene="sdhC"
                     /locus_tag="ACHKFK_002534"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(10994..11398)
                     /gene="sdhC"
                     /locus_tag="ACHKFK_002534"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO9052848.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPVTAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQAFAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            11779..11886
                     /locus_tag="ACHKFK_002535"
     CDS             11779..11886
                     /locus_tag="ACHKFK_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052849.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            12092..13375
                     /gene="gltA"
                     /locus_tag="ACHKFK_002536"
     CDS             12092..13375
                     /gene="gltA"
                     /locus_tag="ACHKFK_002536"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO9052850.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            13503..13895
                     /locus_tag="ACHKFK_002537"
                     /pseudo
     CDS             13503..13895
                     /locus_tag="ACHKFK_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            14047..15093
                     /gene="abrB"
                     /locus_tag="ACHKFK_002538"
     CDS             14047..15093
                     /gene="abrB"
                     /locus_tag="ACHKFK_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9052851.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLALHLPAALLLGPMIAGIIFSMRG
                     VTLQLPRSAFLADQAILGCMIAQNLTGSILTTLAVNWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCLLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(15090..15881)
                     /gene="nei"
                     /locus_tag="ACHKFK_002539"
     CDS             complement(15090..15881)
                     /gene="nei"
                     /locus_tag="ACHKFK_002539"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO9052852.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKSYQSRLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRLSYATRGQVDE
                     NKYHGALFRFKVFHRDGEPCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(15917..16651)
                     /gene="pxpA"
                     /locus_tag="ACHKFK_002540"
     CDS             complement(15917..16651)
                     /gene="pxpA"
                     /locus_tag="ACHKFK_002540"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO9052853.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVFAQTLYQIGALAAITRAQGGVMCH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGERYGLVTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWAMVTAQTVCLH
                     GDGEHALAFARRLRATFAEKGIVVAA"
     gene            complement(16641..17573)
                     /gene="pxpC"
                     /locus_tag="ACHKFK_002541"
     CDS             complement(16641..17573)
                     /gene="pxpC"
                     /locus_tag="ACHKFK_002541"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO9052854.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMRAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLRDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(17567..18223)
                     /gene="pxpB"
                     /locus_tag="ACHKFK_002542"
     CDS             complement(17567..18223)
                     /gene="pxpB"
                     /locus_tag="ACHKFK_002542"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO9052855.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVNMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAAGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLHVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPERDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(18246..18989)
                     /gene="ybgI"
                     /locus_tag="ACHKFK_002543"
     CDS             complement(18246..18989)
                     /gene="ybgI"
                     /locus_tag="ACHKFK_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO9052856.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQDLLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            19260..20741
                     /gene="dtpD"
                     /locus_tag="ACHKFK_002544"
     CDS             19260..20741
                     /gene="dtpD"
                     /locus_tag="ACHKFK_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO9052857.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYSD
                     NHAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGF
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(20783..22201)
                     /gene="phrB"
                     /locus_tag="ACHKFK_002545"
     CDS             complement(20783..22201)
                     /gene="phrB"
                     /locus_tag="ACHKFK_002545"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO9052858.1"
                     /translation="MTTHLVWFRQDLRQHDNLALAAACRNSSARVLALYIATPRQWAA
                     HNVSPRQAELINTQLNALQNALAEKGIPLLFREVDDFVASVEIVKQVCAENRVTHLFY
                     NYQYEVNERARDVQAERTLRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQVLEGGPGSVWLNEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLKAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRQWLPELRDVPGKAVHEPWKWAEKTGVTLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(22198..22707)
                     /gene="ybgA"
                     /locus_tag="ACHKFK_002546"
     CDS             complement(22198..22707)
                     /gene="ybgA"
                     /locus_tag="ACHKFK_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO9052859.1"
                     /translation="MNQQLFDDSTLIRIFALHELHNLKDRGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHRWQSLDDYYNQYRQRVIVLLSHPANRRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQSLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHAGVL"
     gene            complement(22808..23014)
                     /gene="ybfA"
                     /locus_tag="ACHKFK_002547"
     CDS             complement(22808..23014)
                     /gene="ybfA"
                     /locus_tag="ACHKFK_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO9052860.1"
                     /translation="MELYKEYPAWLIFLRRTCAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATSDFY"
     gene            23327..23416
                     /gene="kdpF"
                     /locus_tag="ACHKFK_002548"
     CDS             23327..23416
                     /gene="kdpF"
                     /locus_tag="ACHKFK_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO9052861.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            23416..25089
                     /gene="kdpA"
                     /locus_tag="ACHKFK_002549"
     CDS             23416..25089
                     /gene="kdpA"
                     /locus_tag="ACHKFK_002549"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO9052862.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFSALGVSDREMNWKQYLSAILGLNILGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     NTLGNAWVDLLRITLWVLTPVALLIALFFIQQGVLQNFLPYQAVTTIEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVAGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLALCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            25112..27160
                     /gene="kdpB"
                     /locus_tag="ACHKFK_002550"
     CDS             25112..27160
                     /gene="kdpB"
                     /locus_tag="ACHKFK_002550"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO9052863.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGVMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVEEKALA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGIDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            27169..27741
                     /gene="kdpC"
                     /locus_tag="ACHKFK_002551"
     CDS             27169..27741
                     /gene="kdpC"
                     /locus_tag="ACHKFK_002551"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO9052864.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPNASTNVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            27734..30418
                     /gene="kdpD"
                     /locus_tag="ACHKFK_002552"
     CDS             27734..30418
                     /gene="kdpD"
                     /locus_tag="ACHKFK_002552"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO9052865.1"
                     /translation="MNNEPLRPDPDRLLEQAAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLKGLAVLPLKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLITL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDAPPARTLNNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSSQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGINAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            30415..>31074
                     /gene="kdpE"
                     /locus_tag="ACHKFK_002553"
     CDS             30415..>31074
                     /gene="kdpE"
                     /locus_tag="ACHKFK_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO9052866.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATTTPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IG"
BASE COUNT         7003 a         8401 c         8087 g         7583 t
ORIGIN      
        1 agataataaa acagtgaatg atggataact tgaccagaaa aacaagcaag catataaaat
       61 taaaaaatat gattccactt tcatttttat aaaatggcat cgtgaagagc gagttgataa
      121 tgctgcaatg catcagcgtc ctgtccagca gtttcataaa ttttggcaat gttataatag
      181 gagttagcgc gcgttgtcgc cgcatttgct ccactggcta acgagatcgc tttacgattt
      241 gcccaaattg cttccgcgat gttgccattt ttctgatacg ccagccccag gttactaaat
      301 gcctgtccgt aattgccgtc taattctgtt gcctgttgga agagttgaat ggcttgctca
      361 agttgaccgg ttttataaac agcttcacct tcgcgattat attttcttgc cagttgagta
      421 tctccactgt tttgcatcgc aggcgaagcc ggagctgaag agtccgtcgc aggcattatc
      481 acagtggcta aatccctgac gtctaatgtg gtgtcgttaa aattggcgct ggtaacagag
      541 ttgatatctt caaactcgcc gtttggattg agtttaaaca cggtccagat gttccctcgt
      601 tttccagcag ggacactata actacgcacc agggagctcc ctacataaac aaacactttt
      661 gccttactgg cagagagata attactgttg ggcaaccctt tattgctata gtcctgcacg
      721 gcatagatgt aactttcgcc aaaatgcttt ttgctgatag tgacagtttc tggtccatag
      781 ctatccgtat catcaacatc cagattagca tcagtcccct ctttactatc aaaatagata
      841 tgtccgccag agaagatcaa atgtgaatca agatcaaaag gcttttcgcc ccagctcaaa
      901 actacacgca tgccatccag actagtcatt gccggactaa tcgcataagt catgccagca
      961 cacgaacatt tgaccaccag attagaatag ccctcttttt tgacgatcaa taaagcatct
     1021 tgatcatcag caaatgtaga acccagatta acgctgcccg atgcatttgt ggttccagaa
     1081 acagattgtg cgccattgcg ttgcagcgtc accgatactc cctcaatgcg tttatctttc
     1141 actgtcgcac ttaaaatatc gatatttaca tcggccgcat aaacgtccga atttacaccc
     1201 atcagggcca gagcaaccgg cagaacaatt ttcttcttgt ataaatgctt ttacagaacg
     1261 gagaggggat attttgccgg gcattattgc gcagaagatg ggagaaaagc agtgggcaaa
     1321 cttttcagat tagtgcccga ataaaaaagg ccatcataag atggccaacc atgtcgaaac
     1381 cgacagccct tcgcccctcc tgtagaggga agcggcgagg gctatttctt attacagata
     1441 tttatttcag aacagttttc agtgcttcac cgatatccgc caggctgcga acggttttca
     1501 cgcctgcggc ttccagagca gcgaatttct catccgcagt ccctttacca ccggcaatga
     1561 tcgcacctgc gtgacccata cgtttgcctt tcggcgcagt cacaccagcg atgtaaccca
     1621 caactggctt ggtaacgtgc tccttgatgt acgccgctgc ttcttcttca gcgctaccgc
     1681 cgatctcacc gatcatcacg atcgcttcgg tctgcggatc tttttcgaac atttcgagga
     1741 tgtcgataaa gttagaacca gggatcgggt cgccgccgat accaacgcag gtcgactgac
     1801 cgaaaccgta atccgtggtc tgtttaaccg cttcataggt cagcgtaccg gaacgggaaa
     1861 cgatacccac tttacccggt ttgtgaatgt gacccggctg gataccgatt ttgcattccc
     1921 ccggggtgat aacgcctggg cagttcgggc cgatcatacg aacgcctgct tcatccagtt
     1981 tcactttcac cgtcagcata tccagcgtcg ggatgccttc agtgatggtg ataatcagtt
     2041 tgatgcctgc atcgatagct tccagaatgg agtctttgca gaacggtgct ggtacgtaga
     2101 taacagaggc ggtagcgcca gtagcagcaa cagcttcacg cacggtgttg aacactggca
     2161 ggccgaggtg ggtagtaccg cctttacctg gggttacgcc gccaaccatt ttagtgccgt
     2221 atgcaatggc ctgctctgag tggaaagtcc cctggctacc ggtaaagccc tggcagataa
     2281 ccttggtgtt tttatcaatt aaaatggaca ttatttcccc tccactgcgg caacaacctg
     2341 ctgagctgca tccgtcagac cttttgctgc aataatattc aggccgctgt cagccagttt
     2401 cttcgcgccg agttcggcgt tgttaccttc cagacgtacc acgaccggta cgttaacacc
     2461 cacttctgct accgcgccga tgataccgtc agcgatcagg tcgcaacgaa cgataccgcc
     2521 gaagatgtta accagaacgg ctttcacttt gtcgtcagag aggatgattt tgaacgcttc
     2581 ggttacacgt tctttggttg cgccgccgcc aacgtccagg aagttagccg gttcgccgcc
     2641 gtgcagttta acgatgtcca tcgtacccat cgccagacct gcgccgttaa ccatacaacc
     2701 gatgttaccg tccagcgcaa cgtagttcag ttcccactgt gcagcctgtg cttcacgcgg
     2761 atcttcctgc gactggtcac gcatttcacg cagatcaggc tggcggaaca gtgcgttgcc
     2821 atcagcgccc agtttgccgt cgaggcaaat cagatcgccc tgtttggtaa taaccagcgg
     2881 gttgatttcg ataagcgcaa ggtcgcgctc caggaaaatg gtcgccaggc ccatgaagat
     2941 tttggtgaac tgctgaacca gtttaccttc cagacccagt ttaaacgcca gttcgcgtcc
     3001 ctgatacggc atcgggccag tcagcggatc aagcgcaact ttgtggatca ggtgcggagt
     3061 ttcttccgcc actttttcga tttccacgcc gccttcggtg gaggccataa agaccacgcg
     3121 acgggaacta cggtcaacaa cggcaccgag atacagctct ttagcgatat cggtcgctgc
     3181 ttcaaccaga atctggttaa ccggttggcc attagcatct gtttgatacg ttaccagacg
     3241 cttgcccagc cagttttctg caaaagcacg gatgtcttct ttgctgttta caactttcac
     3301 accgcccgct ttaccgcggc caccagcgtg aacctgacat ttcactaccc acggaccggc
     3361 accgattttt gaagcggctt cttctgcttc gcgcggagta gtacaggcat aacccaccgg
     3421 tgctggtaag ccatagcggg caaaaagttg ttttgcctga tattcatgta agttcatgtg
     3481 ttctgtccat ccttcagtaa tcgttatctt ttaaaccgta gaccgacacc ctatccacgt
     3541 cggtctacag tgcaggtgaa gcttaaacta ctaaacgtcc agcagcagac gcgtcggatc
     3601 ttccagcaac tctttgatcg ttacaaggaa gcccacggat tcgcgaccat cgatcaaacg
     3661 gtgatcgtag gacagcgcca ggtacatcat cggcaggatc tcaacctgac cattcactgc
     3721 catcggacga tctttgatag cgtgcatacc cagaattgcg ctctgcggtg ggttgatgat
     3781 aggcgttgac atcagggaac cgaacacacc accgttggtg atggtgaagt taccaccggt
     3841 cagatcttca accgtcagct tgccgtcacg gcctttaaca gccagctctt tgattttctt
     3901 ctcgatgtct gccatgccga gggtatcgac atcacgcaga accggcgtca ccagaccgcg
     3961 cggcgtagaa accgccatac tgacgtcgaa atagttgtgg tagaccacat catcaccgtc
     4021 gatagacgca ttcacttccg ggtaacgttt cagggcttca accaccgctt tcacgtagaa
     4081 ggacataaag cccagacgga tgccgtggcg tttttcaaac gcttcaccgt actgcttacg
     4141 cagatccata atcggcttca tgttgacttc gttgaacgtg gtcagcatgg cggtggagtt
     4201 tttcgcttcc agcagacgct ctgccacacg cttacgcagg cgagtcatcg gcacacgttt
     4261 ttcgctacgt gcagccaggg ccggttgcgc cgccggagca ggcgctgccg gagcagactc
     4321 tttcgccggg gctttcgcca gatgtttttc cacatcttca cgggtcagac gaccacccac
     4381 acccgtgcct ttaatagcgc tggcgtcgag gttatgttca gccagcaggc gacggatcgc
     4441 cgggcttaac gcatcgttgt tttgctcttc cagagacgcc tgctggcgtt gcgccggagt
     4501 ggacgctttc tcttcagatt tggcgctggt ttctttaccc gcgctgttgc cttcacgcag
     4561 gcgaccaagg atctgacgag acgttaccgt tgtaccttca tcttccagaa ccgcatccag
     4621 aatgccgtct gctgatgccg gtacttccag taccactttg tcagtttcga tttctaccag
     4681 cacttcatca cgtacgactg cgtcgccggg ttttttatgc caggttgcga cggtggcatc
     4741 ggctacggat tcaggcaggt cagggaccag aatatctacg ctactcattg tgtatccttt
     4801 atttattcga cgttcagcgc gtcattaacc agatcttgtt gctgtttctg gtgaacggac
     4861 atatacccta ccgccggaga ggcggaggct gggcggcctg cataacgcag agaagcccca
     4921 aacggaatca cttcacggaa atgatgctgg ctgcagtacc atgcgccctg gttgagcggc
     4981 tcttcctggc accagacaaa atccttgacg tgagcaaact gctgcaacac ttcctgcatc
     5041 gctttatgcg ggaacgggta gagttgctcg atacgcacaa tggcgacatc gtgttgattg
     5101 ttcttacgac gttgttccag caggtcgtaa taaaccttac cagaacacat cactacgcgc
     5161 ttcacgccct tcggatcaag ctcgtcgatt tcaccgatgg ctggcaggaa ggtgccgttc
     5221 gccagttctt cgaggctgga taccgccagc ggatgacgca gcagggattt cggcgacatc
     5281 acgaccagtg gacgacgcat cccgcgcaac gcctgacgac gcagcatgtg gtaaacctgt
     5341 gccggggtag aaggtacgca aacctgcatg ttttgctcag cacaaagttg cagataacgt
     5401 tccagacgcg cggaggagtg ctccggcccc tgcccttcgt aaccgtgcgg cagcaacatc
     5461 accaggccac acatccggcc ccatttctgt tcgccagagg agatgaactg gtcgataacc
     5521 acctgtgcac cgttggcgaa gtcaccgaac tgtgcttccc agatggtcag ggtgcgtggt
     5581 tctgcggtgg cataaccgta ttcaaacgcc agtacggctt cttcagacag tacagagtcc
     5641 cagacgcgga acgcgccctg gccgttatgg atatgttgca gcggcgtgta agtggaaccg
     5701 ttagactggt tgtggatcac cgcgtggcgg tggaagaagg taccgcgacc ggagtcttca
     5761 cccgacaggc gaaccggaat gccttcgtca accaacgtgg cgtaagcgag gttttccgcg
     5821 ccgccccagt cgaacagttt ctcaccggct gccatcgcct ggcgatcgcc ataaatcttg
     5881 gcaacgcgag actgcatttc aactgcttcc ggcaccgtgc tgatgcgttt agccagttcc
     5941 tgcaggcgct tcatctcaac tttgttcggg tactcttcgt cccattcgtg gttgaggtac
     6001 ggcgaccagg tgaaagagtg catgttcatc ggacgccact ctgctacaac gcaatcgcca
     6061 gcatccagcg catcgcggta caggttaacc atctcggtgg catcttccag cgtcgcgact
     6121 ttttcctgct ccagcttgtc agcgtagatt ttgcgcggcg tcggatgttt tttgattttc
     6181 tgatacatca gcggctgggt tgcgctcggc tcgtcggctt cgttgtggcc gtgacggcgg
     6241 tagcatacca ggtcgatgaa gacatcacgt ttaaaggtgt tacggaaatc gagcgccaga
     6301 cgggtaacaa aggcaacggc ttccggatca tccgcattaa cgtggaaaat cggtgcctga
     6361 accatcttac cgatatcagt acagtacggc gtcgaacggg catccagcgg gttagaggtg
     6421 gtgaagccaa cctggttgtt gataacgata cgtaccgtac cgccaacttc ataaccacgc
     6481 gctttcgaca tgttcagggt ttcctgaact acgccctgcc cggtcactgc ggcgtcacca
     6541 tgaatggtga ttggcagcac tttgttgctg ctcggctcat caagtctgtc cagacgggca
     6601 cgaacagaac cgataacgac cgggcttaca atctcaaggt gagacgggtt aaacgccagc
     6661 gccaggtgca ccaggccgcc atcggtctgg aagtcagacg agaagcccat gtggtatttc
     6721 acgtcgcccg tgccgaggtg ttctttatgt ttaccggcaa actcgtcgaa caagtcttgc
     6781 ggttttttac ccagcacgtt caccagcacg ttcagacgac cacggtgcgc catcccgaga
     6841 accacttcgc gtgtgccgct gttgccagcg tggcggatca tctctttaag catcgggatt
     6901 aacgcgtcac cgccttccag cgagaagcgt tttgcgccag ggaattttgc gccaaggtaa
     6961 cgttcaaggc cttcagcggc ggtcagttcg cttaagaagc gttttttctc ttcgctattg
     7021 aaagtcgcgc gaccagactc aatacgctgt tggatccagc gtttttcttc agtgctggta
     7081 atgtgcatat actcggcacc aatcgggccg cagtaggttt gcttgagtgc ttccagcagt
     7141 tcgccgagtt tcatggtttc tttgccgctg gcaaatgaac cgacgttgaa ggtctcctgg
     7201 aagtctgctt cggtcagatc gtggaaagac ggatccagat cggccacttt atcttgctgc
     7261 cacagtccca gcggatcgag attcgcatgc tggtgaccac ggaagcggta tgcgttaatg
     7321 agctgcagga ctttaacctg cttcacattg gtgtcagggt cggagatcgt tgaagagtaa
     7381 cgtgaagcgt ctttcgccag gcggcggaaa tattcacgcg tttgagagtg gaattgatcc
     7441 ggtttgactc ccgtaccagg taactgctgg aacgtcgaac gccagttagc gtcaaccgag
     7501 tcaggatcgg ttaagaagtc ttcatagagc tgttctatcc agctctggtt tgcgccagag
     7561 aggtaagaag agtccaacca ggctttcaaa gcgctgttct gcatcgtgat cccttaagca
     7621 tcttttttat gcttacttcg ccgtggatac taccacgcac atttcatatc tgatgtgcgt
     7681 gccggggttc acttgttgcg gacttgtcgt gttttctgcg tccgctaagg aacctttaaa
     7741 aactgtctta gtgtcagggg cagtttttaa agatttcctg catttctttt gtaggccgga
     7801 taaggcattc acgccgcatc cggcgctgtt tgcctgatgc gacgcttgcg cgtcttatca
     7861 ggcctacggt ttctgcattt cttttgtagg ccggataagg cgttcacgcc gcatccggca
     7921 ctggttgcct gatgcgacgc ttgcgcgtct tatcaggcct acggtttctg catttctttt
     7981 gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc gacgcttgtg
     8041 cgtcttatca ggcctgcggt ttctgcattt cttttgtagg ccggataagg cgttcacgcc
     8101 gcatccggca ctggttgcct gatgcgacgc ttgcgcgtct tatcaggcct aggtttacgc
     8161 attacgttgc aacaacatcg acttgatatg gccgatggcg cgcgtcgggt tcagcccctt
     8221 cggacataca ctgacgcagt tcatgatgct gtgacagcgg aatacactga atgcatcgct
     8281 caaaccgtcg aggcggctgt cagtctcggt atcacggcta tcgatcagga aacgatatgc
     8341 cgctaacaag cctgccgggc cgataaactt atcgggattc caccagaaag acggacaaga
     8401 ggttgaacaa catgcgcaga gaatacattc atacaacccg tcgagttttt cgcgctgctc
     8461 tggcatctgt aaatgctcgc gagctggcgg attttgtcca ttattcaaca ggtaaggctt
     8521 aattttctca tattgcgcat agaattgtcc catgtctacc accaaatcgc ggatcaccgg
     8581 taaacctggc agcgggcgaa tcacaatctt cttgcccggc tggttgagtg ccgaaatcgg
     8641 agtaatacag gccagaccat tcttaccgtt catgttcaga ccgtcggaac cgcacacacc
     8701 ttcacggcag gagcggcgga acgacaggct gggatctttc tctttcagct gaataagcgc
     8761 atccagcagc atcatgtcgc gaccttcttc cgcttccagg gtgtaatcct gcatacgcgg
     8821 agcatcatca acatccgggt tatagcgata aattgaaaac tcgagtctca ttttcctgtc
     8881 tccgcattag taagtacgaa tcttcggcgg gaatgccggg cgcagtttcg gttccatgtt
     8941 gacgcttcgg cgcgtcatgg attccgactc tggcagatac agggagtggc acagccagtt
     9001 ttcatcatcg cgatccggga agtcgaagcg gctatgcgcg ccacggcttt cggtacggaa
     9061 gttggcagaa acagccgttg catacgccgt ttccatcagg ttatccagtt ccaggcactc
     9121 aacgcgctgg gtgttgaact cgctggaagt gtcatccaga cgggcatttt tcagacgctc
     9181 gcggataact ttcaactgct caagcccttt cgccatcgca tcaccttcac ggaagaccga
     9241 gaagttatgc tgcatacatt cttgcagtgc tttacggatc gccaccggat cttcaccgtt
     9301 acggttattg ttccagcggt tcaggcgatc cagagacgct tctacatcag actcgctggc
     9361 atcgcgcagt gcgccctgct cggcaataga ctcttgcaga tgcagacctg ccgcacgacc
     9421 aaatacgacc aggtccagca gcgagttgcc gcccagacgg ttagcgccat gtaccgatac
     9481 acaagcgatt tcaccaacgg caaacagccc cggaacaacc acatcttcgc ctttctcatt
     9541 cacagtcagc gcctgaccgg tcactttggt cggaataccg cccatcatgt agtgacaggt
     9601 tggaattacc ggaatcggct ctttcaccgg atcaacgtga gcgaaggtgc gggagagttc
     9661 cagaataccc ggcagacggg attcgataac ttctttcccc agatggtcaa gtttcagttt
     9721 tgcgtgtggc ccccacggac catcacagcc gcggccttca cggatttcga tcatgatgga
     9781 acgcgccacc acgtcacggc ccgccaggtc tttggcgttc ggcgcataac gctccataaa
     9841 gcgttcgcca tgtttgttca gcagataacc gccttcacca cggcaacctt cggtgaccag
     9901 tacgcccgca ccggcaatac cggtcgggtg gaactgccac atttccatat cctgtaccgg
     9961 tacgcctgca cggatagcca tgccgacacc gtcgccagtg ttaatgtggg cgttggtggt
    10021 ggactgataa atacgccctg ccccgccagt cgccagcact gtcgcgcgag ctttaaagta
    10081 aaccacttca ccagtttcga tgcacagtgc ggtacaaccg accactgcgc catcctggtt
    10141 tttcaccaga tccaacgcat accactcgga gaaaatggtg gtgtggtttt tcaggttctg
    10201 ctgataaagc gtgtgcagca gtgcgtgacc ggtacggtcg gcagccgccg cagtacgtgc
    10261 cgcctgctcg ccgccgaagt ttttcgactg accgccaaac ggacgttgat agatacgacc
    10321 atcatcaaga cgcgagaacg gcaggcccat atgttccagt tccagaatcg cttccggccc
    10381 ggttttacac atatattcaa tcgcgtcctg gtcaccgata tagtccgacc cttttacggt
    10441 gtcgtacata tgccattccc agttatcttc atgggtatta cccagcgcaa cggtaatacc
    10501 accttgcgca gaaacggtat gggaacgggt cgggaagact ttagagagca gcgcacaggt
    10561 ctggccactc tgggaaattt gcagcgccgc gcgcatacct gcgccgccag caccaatcac
    10621 aactgcatca aattctctga ctggcaattt catcacacac cccacaccac aacgaatcca
    10681 taaatcacgt aaaccaccag tgcaacgaca atcaccagtt gcagcatcag gcgcaaggcc
    10741 agcggtttaa cgtagtcggt caacacctgc cacatgccga tccaggcatg gatcaagata
    10801 gaaaacagcg ccagcagggt gaacactttg gtgaacgcag aggcgaagaa accaatccag
    10861 acttcatatg tcagctcgcc actggtagcg aaaaaaccga ccatataaat gatgtagagc
    10921 gtcaggacga tagcggtagc acgaacgagg atgaaatcat gtacgccatt gcgtcctaat
    10981 gcggaggcgt tgcttaccat acgaggactc ctgcgagaag tgaaagcacg acagtaataa
    11041 caaaggagat tttggcggag cgtttacccg cttcgaatgt ttcttccaga tagccaaaat
    11101 ccatcatcat gtggcgaata cctacgacga cgtgatatgc cagagcggta aggatgcccc
    11161 acatgataaa tttgacgaag aagctgccca taatcgcgaa agcttgctcg aaaccttcag
    11221 gggaagagag gctggtaccc agaagccaca gcaggatgcc cactgcaaca aaggtgatca
    11281 caccggaaac gcgatggaga atggacgcta tcgccgtgac ggggaaccgg atggtctgta
    11341 ggtccagatt aacaggtctt tgttttttca catttcttat catgaataac gcccacatgc
    11401 tgttcttatt attccctggg gactacgggc acagaggtta actttctgtt accaggagac
    11461 gtcgggattt ccttcctccg gtctgcttgc gggtcagaca gcgtcctttc tataactgcg
    11521 cgtcatgcaa aacactgctt ccagatgcga aaacgacacg ttacaacgct gggtggctcg
    11581 ggattgcagg gtgttccgga gacctggcgg cagtataggc tgttcacaaa atcattacaa
    11641 ttaacctaca tatagtttgt cgggttttat ccagaacagt gatctgcgtc acgataacaa
    11701 catttattta atttttaatc atctaatttg acaatcattc aacaaagttg ttacaaacat
    11761 taccaggaaa agcatataat gcgtaaaagt tatgaagtcg gtatttcacc taagattaac
    11821 ttatgtaaca gtgtggaagt attgaccaat tcattcggga cagttattag tggtagacaa
    11881 gtttaataat tcggattgct aagtacttga ttcgccattt attcgtcatc aatagatcct
    11941 ttacctgcaa gcgcccagag ctctgtaccc aggttttccc ctctttcaca gagcggcgag
    12001 ccaaataaca aacgggtaaa gccaggttga tgtgcgaagg caaatttaag ttccggcagt
    12061 cttacgcaat aaggcgctaa ggagacctta aatggctgat acaaaagcaa aactcaccct
    12121 caacggggac acagctgttg aactggatgt gctgaaaggc acgctgggtc aagatgttat
    12181 tgatatccgt actctcggtt caaaaggtgt gttcaccttt gacccaggct tcacttcaac
    12241 cgcatcctgc gaatctaaaa ttacttttat tgatggtgat gaaggtattt tgctgcaccg
    12301 cggtttcccg atcgatcagc tggcgaccga ttctaactac ctggaagttt gttacatcct
    12361 gctgaatggt gaaaaaccga ctcaggaaca gtatgacgaa tttaaaacta cggtgacccg
    12421 tcataccatg atccacgagc agattacccg tctgttccat gctttccgtc gcgactcgca
    12481 tccaatggca gtcatgtgtg gtattaccgg cgcgctggca gcgttctatc acgactcgct
    12541 ggatgttaac aatcctcgtc atcgtgaaat tgccgcgttc cgcctgctgt caaaaatgcc
    12601 gaccatggcc gcgatgtgtt acaagtattc cattggtcag ccatttgttt acccgcgcaa
    12661 cgatctctcc tacgccggta acttcctgaa tatgatgttc tccacgccgt gcgaaccgta
    12721 tgaagttaat ccgattctgg aacgtgctat ggaccgtatt ctgatcctgc acgctgacca
    12781 tgaacagaac gcctccacct ccaccgtgcg taccgctggc tcttcgggtg cgaacccgtt
    12841 tgcctgtatc gctgcaggta ttgcttcact gtggggacct gcgcacggtg gtgctaacga
    12901 agcggcgctg aaaatgctgg aagaaatcag ctccgttaaa cacattccgg aatttgttcg
    12961 tcgtgcgaaa gacaaaaatg attctttccg cctgatgggc ttcggtcacc gcgtgtacaa
    13021 aaattacgac ccgcgcgcca ccgtaatgcg tgaaacctgc catgaagttc tgaaagagct
    13081 gggtaccaaa gatgacctgc tggaagtggc tatggagctg gaaaacatcg cgctgaacga
    13141 cccgtacttt atcgagaaaa aactgtaccc gaacgtcgat ttctactctg gtatcatcct
    13201 gaaagcgatg ggtattccgt cttctatgtt caccgtgatc ttcgctatgg cacgtacggt
    13261 tggctggatc gcccactgga gcgaaatgca cagtgacggt atgaagattg cccgtccgcg
    13321 tcagctgtat acaggatatg aaaaacgcga ctttaaaagc gatatcaagc gttaatggtt
    13381 gattgctaag ccgttcactt tccggacccg cctttaatag agcgggttct ttttatatgg
    13441 ctgaacataa aaattgtaaa aattaaacca gatgcaaata cccattaata aggtgaaaaa
    13501 atatttcacc ggaaacactg gtggttgatt ttggtaaatt taacttactg gatattcgcc
    13561 gccacacaat gtcgaaaaca ttcagtatca agacaacaaa atcacaaaat gaccagtgta
    13621 ccgacggatt taaggtcagc tcctcgtttt ataccgaaaa aacgttggtt gaagaagata
    13681 aagcattgtt gattggtaat ggtctgaagc tacgtatact ggatgaaaac gcctcgcctt
    13741 atacattcaa taaatatgct gaatacgccg atttcaccag tgatatgctg atctacgaaa
    13801 aaacctatac ggctgaatta tcgtccacac ctggcactcc catcgcggcg ggtcccttcg
    13861 atacagtggt actttttaag attaactata actgatttaa cacgacgccc ttttcagccg
    13921 gaaagggcaa cgtattcaga ttattgctga ttttcccctt ttccttgatt accgttattt
    13981 tttccagacc cagctttctt tatgctgtat cgatacacca aagataaaat aatgaggaag
    14041 cgaagaatgc ctgtattgca gtggggaatg ttatgtgttc tgtcacttct cctttctatt
    14101 ggcttcctcg ctttacatct cccggcggca ctcctgcttg gcccgatgat cgccgggatc
    14161 atcttcagta tgcgcggagt cactctgcaa cttccccgct ccgcttttct cgctgaccaa
    14221 gccattcttg gctgcatgat tgcacaaaac ctcactggtt cgattctcac cacgctggcc
    14281 gtcaactggc cgatcgtgct tgcgatttta ctggtgacgc tgctttccag cgccatcgtg
    14341 ggttggttat tggtgcgcta tagctcactg cctggaaata ccggggcctg gggttcttct
    14401 cctggcggcg cggcggcaat ggtcgcaatg gcgcaagatt acggcgcaga tattcgcctg
    14461 gtggcgttta tgcaatatct gcgagtgctg ttcgtcgcag gagccgctgt tctggtcacc
    14521 cgcatgatgc tgggcgataa cgctgaagcg gttaaccagc agatcgtctg gttcccgcca
    14581 gtgagcatta atctcctgct tacgattttg ctcgcggtcg ttgccggtac ggcgggatgc
    14641 ttgctacgtc ttccttccgg cacgatgctc atcccgatgc tggcgggcgc agtgctccag
    14701 tctggtcagc tcatcacgat tgaactcccg gaatggctgc tggcgatggc atatatggca
    14761 attggctggc ggattggtct tggtttcgat aagcaaatat tactgcgggc attgcgccca
    14821 ctgccacaaa tcctgctgtc gatttttgct ctgctggcta tttgtgcggg tatggcgtgg
    14881 gggctgaccc ggtttatgca tattgatttt atgaccgcct acctcgccac cagccccggc
    14941 gggcttgata ccgtggcggt catcgccgca gggagcaatg ccgatatggc gctcatcatg
    15001 gcgatgcaaa ccctgcgcct gttcagtatt ttgctgacgg ggcctgccat cgcacggttt
    15061 atttcaacct atgcgccgaa gcgttcggcc tagtgctggc agcctgggca ccagtaaaac
    15121 gggcgcgatg acagcgtggt tttctcaatg atgccgccac agcgttcgca aggttcgcca
    15181 tcacgatgaa aaaccttaaa gcgaaacagc gccccatgat atttgttttc atccacctgg
    15241 ccccgcgtag cgtaagaaag tcgcggaata tccagtaacg cgtgtgcgag tgcatccagt
    15301 tgcgccgcat tgagatcttt cgctttatga tttccggtca acccaacctg ccagaggatc
    15361 tccacccgca aataattgcc aagtcctgcc agaaacgcct gatcgagcag taatccagca
    15421 aactggcggt tacgaaagcg cggcgacaat aatcgttcct ttaccacctc cggcgtcaga
    15481 ttcggatcca gcacatcggg accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc
    15541 ggagtcaaca tctcaatatc cgaggcgcta taaagcagaa tggttttgtc agccgtttgc
    15601 agttttaccc gcagtactcg cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag
    15661 acgccgtaaa gctgattatg gctgtagagc gttaagtcgt tggaaaaatg agttaacaac
    15721 gccttaccac gcgtttccac atgggtaacg tgttgaccga taagtcgtga ttgataagat
    15781 tttaactgcg ggaaggcaaa ccagacatca gttagtggtt tgcctttgat cgccgcctcc
    15841 aggttatccg ctgcacggcg gatctccggg ccttcaggca tggtgatatc cttttttcat
    15901 tctttattaa gcggggttat gctgcgacaa caatcccctt ttcagcaaat gtagctcgta
    15961 atcggcgggc gaacgccagt gcgtgctcgc catcgccatg taggcagacc gtttgcgccg
    16021 tgaccattgc ccattcgccg gtgatacttt tgactctgcc gtgttgcacc atctccaacg
    16081 tttgcgccag cgcctgttct tcgttttcaa tcaacgcgcc tggctggctt cgcggcacca
    16141 gcgaaccgtc tgcctgataa ccacgatcgg caaacacttc ctcgcgcgtt accaggccat
    16201 atcgttcgcc tgcacgaatc agctcgcttc ctgccagccc gacgagaatc aatgccggat
    16261 cgcaagcgta taccgctctg gcgatggcgt ctgccagttg tgcctctttc gccgcctggt
    16321 tgtataacat gccgtgcggt ttgacatgac acattacgcc gccttgcgca cgggtaatcg
    16381 ctgccagcgc gccaatttga tacagcgtct gggcgaaaac agtttctggc ggcagctgca
    16441 tggcgctgcg accaaaattt tccctgtcgg gaaaactcgg gtgcgcgcca atcgcgacac
    16501 cgttttttat tgcttcacgc acgcaagcct gcatggtttg cgcatcgcct gcatgaaatc
    16561 cacaggcaat attggcagag gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt
    16621 cgcccagatc ggcgttcagg tcaattttca ttgtgcagcc gccatgctaa ttgttcgaaa
    16681 taacgttgtt gatcttgccg tgctttcagt gcctcttcca gtgaacactg gacaaaatga
    16741 atcggctgac cgagcggaat ttgcgccaga tggtacatat cagcctcaat gatacaggca
    16801 atacgcgggt aaccaccggt ggtttgcgca tcgttcatca acacaatggg ctgcccgtta
    16861 tgcggcacct gcaccacacc tggtaacaaa ccgtgagata acagttcgcg atcggtggtg
    16921 cgttttaaaa tttgcccctg taagcgatag cccatccggt tactttgcga gctaagctgc
    16981 cagggcgaac gccagaatgc atcctgcgag gcgcgatcga actcatgata ttccggcccc
    17041 ggcaaggcgc gaatgcggtt gccccacagc agctgtttaa cgccctgcgc ttccataaaa
    17101 tcacgcttcg ctttgccaat cgggagtcgg tcaccatccc tcagtaaacg gccttccagc
    17161 ccgccaatcc ccactttgag atcggtgctg catgatccca ttaccggcgg aacatcaata
    17221 ccacccgcga ccgccagata actgcgcatc ccgtgctgcg ggcgtttaag cgttaaacgc
    17281 tggcctgctc tcattggcaa tcgccagccg gtccagacgg cgttatcatc cagccgcgct
    17341 tcgcaaccgg caccggtcag ggcaaaccac ccatcagttt cgaactcaac cgttaactga
    17401 ccgagcgtga tctccagcgc gggggcgttg gcgtcattac ccaccagcag gttagcaatg
    17461 cgcagcgcag gcatatccag tgcgccgcag tggctgatac ccgactggcg aaaaccgtga
    17521 cgaccgccat cctgcacagt ggtatacatg cccgcacgaa taatcttcag catactccct
    17581 ccttctgggg tacaaagcgc acgctgtctc ccggacgtaa taagatgggt tcgtcacgct
    17641 ccggatcaaa caggctgagt gaggtatgac caatcaactg ccagccaccc ggcgttgcca
    17701 gcggataaac accggtctgc ggcccgccga tcccgacaga acctgctgga acgtgcaagc
    17761 gcggttcagc gcgccgtggc gtgtgtagtt gttccggcaa actcccgaga tacgggaagc
    17821 ccggttgaaa acctaaaaac cagaccacgt attccacgga ggagtgcaat tcaacaacct
    17881 gtttttcgct caatccacaa tgcgccgcga ccaccgccaa atccggtccg gctgcgccac
    17941 cgtaaaccac cggaatttca ataaagcgag actccggctc cagcgcctca ctctcctccc
    18001 accagcgttg caaacgctct atggcatcca gcgccagcga ctcaggatta cgcaaaatca
    18061 ctgtgatatt gttcatgcca ggaatggctt caaccacatt cggcatattc accagacgct
    18121 gtgccagtcg ccagatccgt ttctggctag ccagcgtcac cggcggttcc agttccagca
    18181 ctaccgccgt ttcacctatc agataacaac gcgctcgttg cactttcgat ccctctgatt
    18241 attcgttatg caggattagg aatatcaata aaggtcacat caagatcggt attttcattc
    18301 agccactcgc tcaatgcgcg aataccacca cgttcagtgg catggtgacc tgcagcataa
    18361 aaatgcaatc cctgctcgcg ggctgaatga atggtctgtt cagaaacttc gccagtaata
    18421 aaagcatcca cgccaaaacg cgcggcgcta tcgataaaac tttgcccgcc gcccgtgcac
    18481 caggcgacgc gctggaccac ctcaggtccg gtatcgccac accataacgg cttacgtccc
    18541 agacgcgctt caatccagga ggccagttcc agtcccggca caggcatggt cagttcgccc
    18601 cacggcacca acggctcaat ttcgcccatg accgtgatcc ccagtaacgc cgccagctgt
    18661 gcgttattgc ccagctcagg atgtgcgtca agcggcaaat gccagccata caggttgata
    18721 tcattcgcca acaatgtttt taagcggtta cgcttcattc cgcgaatgac cggagattcg
    18781 cctttccaga agtagccatg atgcacaatg accgcatccg cgcccagacg cactgcctca
    18841 tcgagcaaat cctggctggc agtgacaccg gtaacaattt tttgcaccgt ctctttgcct
    18901 tccacctgca aaccattcgg cgcgtaatca ctaatcgccg cgctgttcag tttttcgtta
    18961 atcagttgtt ccagttcggt gtttttcatc ttcgcgcctt ccgtattctg atagctacac
    19021 cgttaacata gcgttacctc gtctgttcgt cgatagccaa tcttttgaca tattaagaaa
    19081 caaacccgac gccaaaagac gcaaattgtt tatatcatca cgcttcagct aatgtatatt
    19141 tatgcaatca gacagcatat tgcataaata atcagatctg tatcaaagtt ttaactttaa
    19201 ggaaacacaa gcacgccgcg caactattac gtgacgttgc agtcaattgt ggaattatta
    19261 tgaataaaca cgcatcacaa ccgcgcgcta tttactatgt cgttgcgctg caaatctggg
    19321 aatattttag cttttacggc atgcgtgccc tgctgatcct ctatctcacc aatcaactaa
    19381 aatatagcga taatcacgcc tacgagttat ttagcgccta ctgttcgctg gtatatgtca
    19441 cgccaatcct cggtggcttt ttggcggata aagttctcgg caatcgcatg gcggtgatgc
    19501 tgggggcgtt gttgatggcg atcggtcatg tggtgctggg tgccagtgag atccatccgt
    19561 cattcctcta tctgtccctg gcgattatcg tctgcggcta tggtctgttt aaatccaacg
    19621 tcagttgcct gctcggcgag ctgtatgagc caaccgatcc acgtcgtgat ggcggtttct
    19681 cgctgatgta tgcggcgggt aacgtggggt ctattatcgc acctatcgcc tgtggtttcg
    19741 cccaggaaga gtacagttgg gcgatgggct ttggcctggc ggcggtgggt atgatcgcgg
    19801 gtctggtcat tttcttatgt ggcaatcgtc atttcacaca tacccgcggc gttaacaaaa
    19861 aggtactgcg tgcgacaaac tttctcctgc ccaactgggg atggctactg gttctgctgg
    19921 tggcaacgcc tgcgctgatt accgtgttgt tctggaaaga gtggtcggta tacgccttga
    19981 ttgtcgcgac gatcattggc cttggggttc tggcaaaaat ttatcgcaaa gcagaaaacc
    20041 aaaaacagcg taaggagctg gggctgattg ttaccctcac cttcttcagc atgttgttct
    20101 gggcattcgc acaacagggg ggtagctcga ttagccttta tatcgaccgc ttcgttaacc
    20161 gcgatatgtt tggttatacc gttccgactg ctatgttcca gtcgattaat gccttcgcag
    20221 ttatgctgtg cggtgtgttc ctggcgtggg tggttaaaga aagcgtcgca ggtaatcgta
    20281 ccgtgcgcat ctggggaaaa tttgctcttg gccttggcct gatgagcgcc ggattctgca
    20341 ttctgacctt aagcgcccgc tggtccgcaa tgtatggtca ctcttctctg ccactgatgg
    20401 tgttaggcct ggcggtgatg ggctttgcgg aactgtttat cgacccggtt gctatgtcgc
    20461 agattacgcg cattgaaatc cccggcgtga ccggcgtatt aaccggcatt tatatgctgc
    20521 tttccggcgc gattgcgaac tatctggcgg gcgtgattgc cgatcagaca tcgcaggctt
    20581 cgtttgatgc ttccggggcg atcaactact ccatcaatgc atatattgaa gtatttgatc
    20641 aaattacctg gggcgcactg gcgtgtgtag gactggtact gatgatttgg ctgtatcagg
    20701 cgcttaagtt cagaaaccgc gcgctggcgc tggagtctta ataagctagc accatgcctg
    20761 ataaaacgct cgctgcgttt tatcaggctc ctttcctcgc ttcttcgtac gctgccagcg
    20821 ttcgcagcct tgcctctttg tgatcgacta tcggttgcgg ataatccaac gtcacacctg
    20881 ttttctctgc ccacttccac ggctcatgca ccgctttccc cggtacatcg cgcagctccg
    20941 gtagccactg gcggataaat tcgccttcgc ggtcaaattt ctcgccctga gttgtcgggt
    21001 tgaaaatacg aaaatacggc gctgcatcag tacccgtaga agcagcccac tgccagccac
    21061 cgttattagc tgccaaatca ccatcaatca actgcgacat gaaatatcgc tcgccttcgc
    21121 gccagtcgat caacaaatct ttcaccagaa aactggctgt aatcatccgt agccggttat
    21181 gcatccagcc agtgctgtta agctgacgca tggcagcatc gacaatcggg tatcccgttt
    21241 tgccctcctg ccaggccttt aaatgggcgg gattgctttg ccactgtaca cgatccgtcc
    21301 aggcaataaa cggacgatgt ttacacagcg aaggataata cgtcatcaaa tggcggtaga
    21361 actcgcgcca tatcagctca ttaagccaga cgctccccgg tccgccttcc agcacctgcg
    21421 gttgttccgc caacaggcga tgtaaacact ggcgaggcga taacccgccc gttgccaggc
    21481 tggcggacaa acgactggtg ccttccactg ccggaaaatc tcgttgttgc tcatactctc
    21541 cggcaccgtt ctggcaaaac tggcgtaatt gcgcaatcgc cgctttttct tccaccggga
    21601 aatgcgcagt atcgaaagac tgacgaggat aattcagcgt aatggatggc gcgggctcta
    21661 tcgatccgct actacgaact tttggtgcag caacgcactc cggcatccct tcccgcagcc
    21721 gtttcaacca tgcgttttta aaaggcgtaa agactttgta catctcatga ttgccagtca
    21781 tcaccgcccc cggcggcagg atcacgctgt catcaaatcc ttcacacacc acgttacgca
    21841 gcgtcctctc agcctgcaca tcccgcgccc gctcattcac ctcatactga tagttataaa
    21901 acagatgggt aacacgattt tccgcgcaca cctgtttaac tatttcgacg ctggcgacaa
    21961 agtcatccac ttcacggaac aataaaggaa tacctttttc cgccagcgcg ttttgcagcg
    22021 cattaagttg tgtattgata agttcagcct gacgcggcga cacattatgc gccgcccact
    22081 ggcgtggtgt agcgatatac aacgccagca cgcgcgcaga cgaattgcgg caggcggcag
    22141 ccagcgcgag attatcgtgc tgacgtaaat cctggcgaaa ccagaccaga tgggtagtca
    22201 taaaactcct gcatggcgca agttaatcac acgcggccaa agttcgaaat aacgctgccc
    22261 tgaaagccag gcgtcaggat aaagcgccat atagtgtttg atacgcatca gcggcgcaag
    22321 aagcgattgc tcgccacggc gataactgtc gataagcgcc gccagctgct ggcgttctgt
    22381 ggaatcaata tgtgggcgaa aatatccctg aacgtgcatc aaaacgttag tatgatcacg
    22441 ccggttggca gggtgagaaa gcaaaacaat cacgcgttgg cggtactggt tgtaatagtc
    22501 atccagactt tgccaccggt gaatatcagc cacaaacggg ccaagtttgc ggtactccgg
    22561 ctgagaatgc gccagaaaga cgagtttata gcggctgtga taatcgagaa gcgccccgcg
    22621 cgttagtcct cgatctttga gattgtgtaa ctcatgcaaa gcaaaaatgc ggataagggt
    22681 gctgtcatca aaaagttgtt gattcattac tgctcctgaa cggtttacag gagtaagtgt
    22741 agacgccgaa aaaagaaaac cgccagcgtt tccaccggcg gttttcttta aacgtcatgt
    22801 ttggcaatca gtaaaaatca ctggttgcct tttcggcctg atccatccac accggtttat
    22861 cgctggtttt cgaccagacg cggtgcaggt agctataaaa gcgggcgcgg tctttccaga
    22921 aaagcatgaa aggcagcgcc agaacgcccg ctgcaaccgc acaagtacgg cgtaaaaaga
    22981 taagccatgc aggatattct ttgtagagtt ccatactttt ctcccccttt tgtatctacc
    23041 cggtgaatag caccggaaaa atgaattcgt ttatctgatg aaaatagtac cgctgtttgt
    23101 gtaattttac tactcatccg accacttatt tttgcttatt gatggtttat ttacattcac
    23161 cgtgtaatta agttacataa aagttaaatt aatactaagt attagttaaa tcatggcttt
    23221 tgccattttt atactttttt tacaccacgc ccgcagattt ttgcgaaatc tttgcagcca
    23281 gaactctacc cttccggtat cacttttagg ccactggagg tgcactgtga gtgcaggcgt
    23341 gataaccggc gtgttgctgg tgtttttatt actgggttat ctggtttatg ccctgatcaa
    23401 tgcggaggcg ttctgatggc tgcgcaaggg ttcttactga ttgccacgtt tttactggta
    23461 ttaatggtgc tggcgcgtcc cttaggtagc gggctggcgc ggctgattaa tgatatacct
    23521 cttcctggta cgacgggcgt tgaacgcgta ctctttagcg cgcttggcgt ctctgaccgc
    23581 gagatgaact ggaagcagta tctctcagcc attcttggcc tgaatatact ggggctggcg
    23641 gtgctgtttt ttatgttgct cggtcagcac tatctgccgc ttaatccaca gcagttgccg
    23701 gggctgtcgt gggatctggc gctcaatacc gccgtcagct ttgtcacgaa taccaactgg
    23761 caatcttata gcggtgaaac cacgttgagc tatttcagcc agatggcggg cttaacggtg
    23821 caaaactttc tttctgccgc cagcgggatt gcggtgattt ttgcgcttat tcgcgcgttt
    23881 acccgccaga gcatgaacac gctgggtaat gcgtgggtag acctgttgcg gattacgtta
    23941 tgggtgttaa cccccgtagc attgctgatc gcactgtttt ttatacaaca aggtgtgctg
    24001 caaaactttt tgccttatca ggctgtgacg accattgaag gagcgcaaca gctattacca
    24061 atggggccag tagcttctca ggaagcgatc aaaatgctcg gaactaacgg cggtggcttt
    24121 tttaatgcca actcgtcgca tccgtttgaa aacccaaccg cactgaccaa cttcgtgcaa
    24181 atgctggcga tcttcttgat cccaacagcg ctgtgctttg cctttggtga agtggcgggc
    24241 gatcgtcgcc aggggcgcat gttgctgtgg gcgatgtcag tgatttttgt catctgcgta
    24301 ggcgtagtga tgtgggcaga agttcagggt aatcctcatc tgctggcgct cggcgcggac
    24361 agcagcatca atatggaagg taaagagagc cgtttcggcg tgctggtcag tagcctgttt
    24421 gcggtcgtga ctacggcggc ttcctgcggc gcggtgattg cgatgcatga ttcgtttacc
    24481 gctcttggtg gcatggtgcc gatgtggttg atgcaaattg gtgaagtggt attcggcggc
    24541 gtcggttcag gcctttacgg catgatgctg tttgtcctgc tggcggtgtt tattgccggg
    24601 ctgatgattg gtcgtacacc ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa
    24661 ctgactgcac tggcgattct ggtcaccccg acgctggtgc tgatgggcgc ggcgctggca
    24721 atgatgaccg acgccggacg tagcgccatg ctcaaccctg gcccgcatgg ttttagcgaa
    24781 gtgctgtatg ccgtgtcatc cgccgctaac aacaacggca gcgcctttgc cggattaagc
    24841 gccaactctc cattctggaa ctgtttactg gcgttgtgca tgtttgtcgg gcgcttcggg
    24901 gtgattatcc cggtgatggc aattgccggt tcgctggtga gtaaaaagag ccaacccgcc
    24961 agctccggca cattgccaac gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg
    25021 ttgctggttg gcgcactgac ctttatccct gccctggcgc ttggtccggt ggcggaatat
    25081 ctctcctgat gatattgagt gagcactgaa tatgagtcgt aaacaactgg cgctattcga
    25141 accaacactt gtcgttcagg cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca
    25201 atggcgcaat ccggtgatgt ttatcgtctg gatcggcagt ctgctgacca cctgtattag
    25261 catcgcgatg gcaagcggtg tgatgcctgg caatgcgctg tttagcgcgg caattagcgg
    25321 ctggctgtgg gtcaccgtac tgttcgctaa tttcgccgag gcgttggcag aaggccgcag
    25381 taaagcgcag gccaacagtc tgaaaggggt gaaaaaaacc gcttttgctc gcaaactgcg
    25441 cgaaccgaaa tatggcgctg cggcggacaa agttcctgct gatcaactgc gtaaaggtga
    25501 catcgtgctg gtagaagctg gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg
    25561 cgcatcggtc gatgaaagcg ccatcaccgg ggaatcggct ccggtgatcc gtgaatccgg
    25621 cggtgatttt gcctccgtca ctggcggcac gcgtattctt tctgactggc tggtgattga
    25681 gtgcagcgtt aaccccggcg agacatttct ggatcggatg atcgcgatgg tggaaggtgc
    25741 gcagcgacgt aaaacgccga acgagattgc cctgaccatt ctcctgattg ccctgactat
    25801 cgtcttttta ctggcaaccg ccacgctgtg gccgttttcc gcgtggggcg gtaatgcagt
    25861 cagcgtaacg gtactggtgg cactgctggt ctgtctgatc ccaaccacta ttggcggcct
    25921 gttgtcagcg attggcgtcg ccgggatgag tcggatgctg ggcgcgaatg ttattgccac
    25981 cagcgggcga gcagttgaag cagcgggtga tgttgacgtt ctgctgctgg ataaaaccgg
    26041 caccatcacc ctcggtaacc gtcaggcgtc agagtttatc cccgcacaag gcgtggagga
    26101 aaaagcgctg gccgacgccg cacaactggc ttcgctggct gatgaaacgc cggaaggccg
    26161 cagtattgtg atcctcgcca agcagcgttt taacctgcgc gagcgcgatg tgcagtcgct
    26221 ccatgccacc tttgtaccgt ttactgcaca aagccggatg agcgggatca acatcgacaa
    26281 ccgcatgatc cgtaaaggtt ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg
    26341 tcacttccct gccgatgttg atcaaaaagt cgatcaggtt gcgcgtcagg gagccacgcc
    26401 gctggtggtg gtggaaggtt ctcgtgtgct gggcgttatt gcgctgaaag atatcgtcaa
    26461 aggcggtatt aaagagcgct tcgcccagct gcgtaaaatg ggcattaaaa cggtgatgat
    26521 taccggcgat aaccgcctga ctgccgccgc gatagctgcg gaagcgggta tcgatgattt
    26581 tctcgccgaa gcaacaccgg aggccaagct ggcactgatt cgtcagtatc aggcggaagg
    26641 ccgtttagtg gcgatgaccg gcgatggcac caacgatgct ccggcgctgg cacaggccga
    26701 tgttgcggtg gcgatgaact ccggcaccca ggcggcgaaa gaagcgggca atatggtgga
    26761 tctcgactcc aacccgacca agttgatcga ggtagtgcac attggcaaac agatgctgat
    26821 gacccgtggc tcgttgacca ccttcagcat tgccaacgat gtggcgaaat acttcgccat
    26881 tattccagcg gcgttcgcgg caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct
    26941 gcattcgccc gactccgcca ttctcagtgc ggtcattttc aacgccttga ttatcgtctt
    27001 tttgattccc ctggcgttaa aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt
    27061 gcgccgtaac ttatggattt acggtctggg tgggctgctg gtgccgttta ttggtatcaa
    27121 agtcattgat ttactgctaa ccgtttgcgg tctggtgtga ggtttaccat gagtggatta
    27181 cgtccggcat tatcgacatt tctctttctg ttattgatta ctggcggcgt ttacccgctg
    27241 ctgaccaccg cactggggca atggtggttt ccctggcagg ccaacggttc attgattcgt
    27301 gaaggtgata cggtgcgcgg ttcggcatta atcgggcaaa attttaccgg caacggctat
    27361 tttcatggtc gcccgtcggc aacagcagaa atgccctata atccacaggc ttctggcggg
    27421 agcaacctgg cggtcagtaa cccggagctg gataaacaga tagccgcacg cgttgctgca
    27481 ttacgggctg ctaacccgaa tgccagcacg aacgttccgg ttgaactggt gacggcgtca
    27541 gcaagcgggc tggacaataa catcaccccg caagcggcgg cctggcagat cccacgcgtg
    27601 gcgaaagcgc gtaatctcag cgttgaacag ctcacgcaac tgatcgcaaa atacagccaa
    27661 caaccgctgg tgaaatatat cggccagccg gtcgtcaaca ttgtcgaact caatctggca
    27721 ctggataagc ttgatgaata acgaaccctt acgtcccgac cccgatcgtc tgctggaaca
    27781 agctgccgcg ccgcatcggg ggaagctgaa agttttcttc ggtgcctgcg caggcgtcgg
    27841 gaagacctgg gcgatgctgg cggaagccca gcgactgcgg gcgcaagggc tggatattgt
    27901 ggttggcgtg gtagaaaccc acggtcggaa agataccgcc gcaatgctga aaggtctggc
    27961 tgttctgccg ttaaaacgcc aggcgtaccg tgggcggcac atcagcgagt ttgatctcga
    28021 tgccgccctc gcccgccgtc cggcactgat cttaatggac gaactggcgc acagtaatgc
    28081 gccaggttct cgtcatccta aacgctggca ggacatcgaa gagctgctgg aagcaggcat
    28141 tgatgttttc actaccgtca acgttcagca tctggaaagc ctgaacgatg tggtcagcgg
    28201 cgtcaccgga attcaggtac gggagaccgt gcccgatcct ttttttgatg ccgccgacga
    28261 cgtggtgctg gtggacttgc ccccggacga tctgcgccag cgactgaaag aaggcaaagt
    28321 ctatattgcc gggcaggcgg agcgcgccat tgaacatttt ttccgcaaag gtaatctgat
    28381 caccctgcgc gaactggcac tgcgccgtac tgccgatcgc gtagatgagc aaatgcgcgc
    28441 ctggcggggg catcctggcg aagagaaagt ctggcacaca cgcgacgcga tccttttatg
    28501 catcgggcat aacaccggca gtgagaaact ggtccgcgcg gcggcgcggt tggcctcgcg
    28561 cctgggcagt gtctggcacg cggtgtatgt tgaaacccct gccctgcatc gcttaccgga
    28621 gaaaaaacgt cgggcaattc tcagcgcctt acgtctggcg caggagctgg gcgcggagac
    28681 ggcaacactt tctgatccag cggaagaaaa agcggtagtg cgttatgccc gtgaacataa
    28741 tctcggcaag attattctcg gtcgcccggc ctcgcgccgc tggtggcgtc gggaaacatt
    28801 tgctgaccga ctggcgcgta tcgcccccga tctcgatcag gtgctggtcg cacttgatgc
    28861 cccccccgcc cgcacgctta ataacgcgcc ggatagccgc tcttttaaag acaagtggcg
    28921 ggtacagatt cagggatgcg tggttgccgc cgcgttatgc gccgttatca ccttaattgc
    28981 catgcagtgg ctgatggcgt ttgatgccgc caacctggtg atgctgtatc tgcttggcgt
    29041 ggttgtggtg gcactgtttt atggccgctg gccttcggtg gttgcgaccg tcattaatgt
    29101 ggtgagtttc gatctctttt ttatcgcccc acgcggcacg ctcgccgtct ctgatgtgca
    29161 atatctgctg accttcgcgg tgatgttaac cgtcgggctg gtgatcggga accttactgc
    29221 tggcgtgcgt tatcaggcgc gggtagcccg ttaccgcgag caacgcacac ggcacttata
    29281 tgaaatgtcc aaagctctgg ctgtgggccg tagttcgcag gatatcgccg ccaccagcga
    29341 acagtttatt gcctccacgt ttcatgcccg tagccaggtg ttgctgcctg atgacaacgg
    29401 taaattgcag cctttaacac atccgcaagg aatgacgccg tgggacgatg ccatcgcgca
    29461 gtggagtttt gataaaggcc tgcctgcggg cgcgggcacc gacacgttac ccggtgtacc
    29521 gtaccagatt ttgccgctaa aaagcggcga gaaaacctac ggactggtgg tggtggaacc
    29581 ggggaatctg cgccagttga tgatcccgga acagcagcgc ctgctggaga cgtttacgct
    29641 gttagtcgcc aatgcccttg agcgactgac actgaccgcc agcgaagaac aggcgcggat
    29701 ggcaagcgaa cgtgaacaga tccgcaacgc cctgctggcg gcgctttcgc atgatttacg
    29761 cacgccgctt acggtgctgt tcggtcaggc agaaatctta acgctcgatc tggcaagcga
    29821 aggatcgccc cacgcccgcc aggccagcga gattcgtcag catgtgctga acaccacccg
    29881 actggtgaat aatctactgg atatggcgcg aattcagtcc ggcggcttta atttgaagaa
    29941 agagtggtta acgctggaag aagtggtcgg cagcgcgctg caaatgctgg aaccgggttt
    30001 atcgtcgccc atcaatcttt ctctgccaga gccgctgacc ttaatccacg ttgacgggcc
    30061 actctttgaa cgggtgctga ttaacctgct ggagaacgcg gtgaaatatg cgggggcgca
    30121 ggccgaaatt ggtatcaatg cccacgttga gggcgaaaat ctacaactgg atgtctggga
    30181 taacggcccc ggtcttccac caggccagga gcagacgata tttgataagt ttgctcgcgg
    30241 gaataaagag tcggcggtac cgggtgtagg gcttgggctg gcaatttgtc gggcgatagt
    30301 ggatgtacac ggcggcacca ttaccgcatt caaccgaccg gaaggtggtg cctgttttcg
    30361 tgttacactt ccccagcaaa cagcccctga acttgaagaa tttcatgagg atatgtgaca
    30421 aacgttctga ttgttgaaga tgaacaggct attcgtcgct ttctgcgcac ggcgctggag
    30481 ggcgacggta tgcgcgtctt tgaggccgaa acgctgcaac gcggcttgct ggaagcggca
    30541 acccgtaagc cagatttgat tattctcgat ctcggcctgc ccgacggtga tgggattgag
    30601 tttatccgcg acctgcgcca gtggagcgcg gtgccggtga ttgtgctttc cgcacgcagc
    30661 gaagagagcg ataaaatcgc cgcgctggat gccggagcgg atgattatct gagtaagccg
    30721 tttggcattg gcgaattgca ggcccgtctg cgcgtcgcat tacgccgcca ctccgccacc
    30781 accacgcccg atccgctggt gaaattttct gatgttaccg tcgatttagc cgcccgcgtt
    30841 atccaccgcg gcgaggaaga ggtgcatctc acaccgattg agttccgcct gctggccgtg
    30901 ctgctcaaca acgccggaaa agtgctcacc cagcgccagt tactcaatca ggtgtgggga
    30961 ccaaacgcgg tcgaacacag ccactatttg cgtatttata tggggcatct gcgacaaaaa
    31021 ctggagcaag atcccgcccg cccccgccat tttattactg aaaccggtat tggg
//
LOCUS       DAZGJT010000051        30461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZGJT010000051 DAZGJT010000000
VERSION     DAZGJT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.051"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..729
                     /locus_tag="ACHKFK_002554"
     CDS             <1..729
                     /locus_tag="ACHKFK_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFO9052867.1"
                     /translation="EVRELLSQYDFPGDDTPIVRGSALKALEGDAEWEAKILELAGFL
                     DSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKETQKS
                     TCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESEVYIL
                     SKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHPIAMD
                     DGLRFAIREGGRTVGAGVVAKVLS"
     gene            959..1342
                     /gene="secE"
                     /locus_tag="ACHKFK_002555"
     CDS             959..1342
                     /gene="secE"
                     /locus_tag="ACHKFK_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO9052868.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            1344..1889
                     /gene="nusG"
                     /locus_tag="ACHKFK_002556"
     CDS             1344..1889
                     /gene="nusG"
                     /locus_tag="ACHKFK_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO9052869.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEELFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            2048..2476
                     /gene="rplK"
                     /locus_tag="ACHKFK_002557"
     CDS             2048..2476
                     /gene="rplK"
                     /locus_tag="ACHKFK_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO9052870.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2480..3184
                     /gene="rplA"
                     /locus_tag="ACHKFK_002558"
     CDS             2480..3184
                     /gene="rplA"
                     /locus_tag="ACHKFK_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO9052871.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3476..3973
                     /gene="rplJ"
                     /locus_tag="ACHKFK_002559"
     CDS             3476..3973
                     /gene="rplJ"
                     /locus_tag="ACHKFK_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO9052872.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            4040..4405
                     /gene="rplL"
                     /locus_tag="ACHKFK_002560"
     CDS             4040..4405
                     /gene="rplL"
                     /locus_tag="ACHKFK_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO9052873.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4725..8753
                     /gene="rpoB"
                     /locus_tag="ACHKFK_002561"
     CDS             4725..8753
                     /gene="rpoB"
                     /locus_tag="ACHKFK_002561"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO9052874.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8830..13053
                     /gene="rpoC"
                     /locus_tag="ACHKFK_002562"
     CDS             8830..13053
                     /gene="rpoC"
                     /locus_tag="ACHKFK_002562"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO9052875.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYTIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(13295..14428)
                     /gene="thiH"
                     /locus_tag="ACHKFK_002563"
     CDS             complement(13295..14428)
                     /gene="thiH"
                     /locus_tag="ACHKFK_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO9052876.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNVVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAEAEYAELKQLGLDGVMVYQETYHEATYAHHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDSWRVDCYMVVEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQAICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRPSQRP"
     gene            complement(14425..15195)
                     /gene="thiG"
                     /locus_tag="ACHKFK_002564"
     CDS             complement(14425..15195)
                     /gene="thiG"
                     /locus_tag="ACHKFK_002564"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO9052877.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRACGSQLVTLAMKR
                     VNLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEKLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASE"
     gene            complement(15197..15397)
                     /gene="thiS"
                     /locus_tag="ACHKFK_002565"
     CDS             complement(15197..15397)
                     /gene="thiS"
                     /locus_tag="ACHKFK_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO9052878.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQQQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(15381..16136)
                     /gene="thiF"
                     /locus_tag="ACHKFK_002566"
     CDS             complement(15381..16136)
                     /gene="thiF"
                     /locus_tag="ACHKFK_002566"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO9052879.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLMA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINATCVALNTPLITASAVRFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16129..16764)
                     /gene="thiE"
                     /locus_tag="ACHKFK_002567"
     CDS             complement(16129..16764)
                     /gene="thiE"
                     /locus_tag="ACHKFK_002567"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO9052880.1"
                     /translation="MYQPEFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDQR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLSAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16764..18659)
                     /gene="thiC"
                     /locus_tag="ACHKFK_002568"
     CDS             complement(16764..18659)
                     /gene="thiC"
                     /locus_tag="ACHKFK_002568"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO9052881.1"
                     /translation="MSATKLTRREQRAQAQHFIDTLEGSAFPNSKRIYITGTQPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARKGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSVQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGIITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18740..18840)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18892..19368)
                     /gene="rsd"
                     /locus_tag="ACHKFK_002569"
     CDS             complement(18892..19368)
                     /gene="rsd"
                     /locus_tag="ACHKFK_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO9052882.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGVKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDDYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKYPA"
     gene            19463..20236
                     /gene="nudC"
                     /locus_tag="ACHKFK_002570"
     CDS             19463..20236
                     /gene="nudC"
                     /locus_tag="ACHKFK_002570"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO9052883.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQLQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGEIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20276..21340
                     /gene="hemE"
                     /locus_tag="ACHKFK_002571"
     CDS             20276..21340
                     /gene="hemE"
                     /locus_tag="ACHKFK_002571"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO9052884.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVASILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21350..22021
                     /gene="nfi"
                     /locus_tag="ACHKFK_002572"
     CDS             21350..22021
                     /gene="nfi"
                     /locus_tag="ACHKFK_002572"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO9052885.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22064..22654
                     /gene="yjaG"
                     /locus_tag="ACHKFK_002573"
     CDS             22064..22654
                     /gene="yjaG"
                     /locus_tag="ACHKFK_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO9052886.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAVFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22841..23113
                     /gene="hupA"
                     /locus_tag="ACHKFK_002574"
     CDS             22841..23113
                     /gene="hupA"
                     /locus_tag="ACHKFK_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO9052887.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23126..23821
                     /gene="yjaH"
                     /locus_tag="ACHKFK_002575"
     CDS             23126..23821
                     /gene="yjaH"
                     /locus_tag="ACHKFK_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO9052888.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23823..24242)
                     /gene="zraP"
                     /locus_tag="ACHKFK_002576"
     CDS             complement(23823..24242)
                     /gene="zraP"
                     /locus_tag="ACHKFK_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO9052889.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGYGDCGGGGHMGMGHW"
     gene            24480..25856
                     /gene="zraS"
                     /locus_tag="ACHKFK_002577"
     CDS             24480..25856
                     /gene="zraS"
                     /locus_tag="ACHKFK_002577"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO9052890.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGNEEAWRRIDSADGEPVLEIYRQFQPMFATGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERASAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVELNTLISHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVN
                     ITRKDPQG"
     gene            25853..27178
                     /gene="zraR"
                     /locus_tag="ACHKFK_002578"
     CDS             25853..27178
                     /gene="zraR"
                     /locus_tag="ACHKFK_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO9052891.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKTLNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSVDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEIPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LVQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27175..28464)
                     /gene="purD"
                     /locus_tag="ACHKFK_002579"
     CDS             complement(27175..28464)
                     /gene="purD"
                     /locus_tag="ACHKFK_002579"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO9052892.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKVDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHNIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVRDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28476..30065)
                     /gene="purH"
                     /locus_tag="ACHKFK_002580"
     CDS             complement(28476..30065)
                     /gene="purH"
                     /locus_tag="ACHKFK_002580"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO9052893.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRDQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEI
                     DANEGSLTLETRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGENKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERIPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTKRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7127 a         7893 c         8397 g         7044 t
ORIGIN      
        1 gaagttcgtg aacttctgtc tcagtacgac ttcccgggcg acgacactcc gatcgttcgt
       61 ggttctgctc tgaaagcgct ggaaggcgac gcagagtggg aagcgaaaat cctggaactg
      121 gctggcttcc tggattccta cattccggaa ccagagcgtg cgattgacaa gccgttcctg
      181 ctgccgatcg aagacgtatt ctccatctcc ggtcgtggta ccgttgttac cggtcgtgta
      241 gaacgcggta tcatcaaagt tggtgaagaa gttgaaatcg ttggtatcaa agagactcag
      301 aagtctacct gtactggcgt tgaaatgttc cgcaaactgc tggacgaagg ccgtgctggt
      361 gagaacgtag gtgttctgct gcgtggtatc aaacgtgaag aaatcgaacg tggtcaggta
      421 ctggctaagc cgggcaccat caagccgcac accaagttcg aatctgaagt gtacattctg
      481 tccaaagatg aaggcggtcg tcatactccg ttcttcaaag gctaccgtcc gcagttctac
      541 ttccgtacta ctgacgtgac tggtaccatc gaactgccgg aaggtgtaga gatggtaatg
      601 ccgggcgaca acatcaaaat ggttgttacc ctgatccacc cgatcgcgat ggacgacggt
      661 ctgcgtttcg caatccgtga aggcggccgt accgttggcg cgggcgttgt agcaaaagtt
      721 ctgagctaat tgccgataac atttgacgca atgcgcacta aaagggcatc atttgatgcc
      781 ctttttgcac gctttcgtac cagaacctgg ctcatcagtg attttctttg tcataatcat
      841 tgctgagaca ggctctgttg agggcgtata atccgaaaag ctaatacgcg tttcgatttg
      901 gtttgcctcg cgatcgcggg gtgaaaatgt ttgtagaata cttctgacag gttggtttat
      961 gagtgcgaat accgaagctc aaggaagcgg gcgcggcctg gaagcgatga agtgggtcgt
     1021 tgtggtggca ttgctcctgg tagcgattgt cggcaactac ctttatcgcg acattatgct
     1081 gccgctgcgt gcgctggccg tagtaattct gattgctgca gcgggtggtg tcgcgctgtt
     1141 aacgacaaaa ggtaaagcaa ccgttgcttt tgcccgtgaa gcgcgtaccg aagtccgtaa
     1201 ggtcatttgg ccgactcgcc aggaaacatt gcacaccacg ctgattgtgg ctgcggttac
     1261 cgcagtaatg tcactgatcc tgtggggact ggatggtatt ctggttcgcc tggtatcctt
     1321 tatcactggc ctgaggttct gagatgtctg aagctcctaa aaagcgctgg tacgtcgttc
     1381 aggcgttttc cggttttgaa ggccgcgtag caacgtcgct gcgtgagcat atcaaattac
     1441 acaacatgga agagttgttt ggtgaagtca tggtaccaac cgaagaagtg gttgaaatcc
     1501 gtggcggtca gcgtcgcaaa agcgaacgta aattcttccc tggctacgtc ctcgttcaga
     1561 tggtgatgaa cgacgcgagc tggcacctgg tgcgcagcgt accgcgtgtg atgggcttca
     1621 tcggcggtac ttccgatcgt cctgcgccaa tcagcgataa agaagtcgat gcgattatga
     1681 accgcctgca gcaggttggt gataagccgc gtccgaaaac gttgtttgaa ccgggtgaaa
     1741 tggtccgtgt taatgatggt ccgttcgctg acttcaacgg cgttgttgaa gaagttgatt
     1801 acgagaaatc tcgtctgaaa gtgtctgttt ctatcttcgg tcgtgcgacc cctgtagagc
     1861 tggacttcag ccaggttgaa aaagcttaac ccagcgatca aaaaagcggc gatttaatcg
     1921 ttgcacaagg cgcgagattg gaatacaatt tcgcgccttt tgtttttatg ggccttgccc
     1981 gtaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc aacttgagga
     2041 atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag ctggtatggc
     2101 taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca tcatggaatt
     2161 ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga ttccggtagt
     2221 aatcactgtt tacgctgacc gttctttcac tttcgttacc aagactccgc cggcagcagt
     2281 tctgctgaaa aaagcggctg gtatcaagtc tggttccggt aagccgaaca aagacaaagt
     2341 gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg ccgacatgac
     2401 tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt ccatgggcct
     2461 ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta tccgcgagaa
     2521 agttgatgca accaaacagt acgacatcaa cgaagctatc gctctgctga aagaactggc
     2581 gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg acgctcgtaa
     2641 atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc gttccgttcg
     2701 cgtagccgta tttacccagg gtgcaaacgc tgaagctgct aaagctgcag gtgctgaact
     2761 ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact ttgacgttgt
     2821 tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc tgggtccgcg
     2881 cggcctgatg ccaaacccga aagtgggtac tgtaacgccg aacgttgctg aagcggttaa
     2941 aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca tccacaccac
     3001 catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag ctctgctggt
     3061 tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga aagttagcat
     3121 ctctaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg cttctgtaaa
     3181 ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt atgctaacga
     3241 aagttagcag ctaaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag
     3301 accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat
     3361 tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg
     3421 aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc
     3481 tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc
     3541 gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg
     3601 taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg
     3661 tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat
     3721 tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc
     3781 gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca
     3841 gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac
     3901 catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa
     3961 agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg
     4021 atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc
     4081 tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc
     4141 cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga
     4201 attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg
     4261 tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gttgaatctg caccggctgc
     4321 tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc
     4381 tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc
     4441 tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt
     4501 cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac
     4561 tgcgacagga cgcccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca
     4621 ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc
     4681 tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata
     4741 ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt
     4801 atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc
     4861 agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt
     4921 ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc
     4981 aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg
     5041 agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg
     5101 aaattccgct catgacggac aacggtacct ttgttatcaa cggtactgag cgtgttatcg
     5161 tttcccagct gcaccgtagt ccgggggtct tttttgactc cgacaaaggt aaaacccact
     5221 cttcgggtaa ggtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact
     5281 tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactacctg
     5341 cgacaatcat tctgcgtgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg
     5401 aaaaagttat ctttgaaatc cgtgataaca agttgcagat ggaactggtg ccggaacgcc
     5461 tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac gtagaaaaag
     5521 gccgccgcat tactgcgcgc catattcgcc agctggaaaa agacgacgtc aagctgatcg
     5581 aagttccggt tgagtacatc gcaggtaaag tggttgctaa agactacatt gatgagtcta
     5641 ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga
     5701 gccagtctgg tcataagcgt atcgaaacgc tgttcaccaa cgatctggat cacggcccat
     5761 atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa
     5821 tctaccgtat gatgcgccct ggcgagccgc cgactcgtga agctgctgaa agcctgttcg
     5881 agaacctgtt cttctccgaa gaccgctatg acttgtctgc ggttggtcgt atgaagttca
     5941 accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa gacgacatca
     6001 ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg
     6061 accacctcgg caaccgtcgt atccgttctg ttggcgaaat ggcggaaaac cagttccgcg
     6121 ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata
     6181 ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct
     6241 tcggttctag ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc
     6301 acaaacgtcg tatctcggca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg
     6361 aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag
     6421 gtccgaacat cggtctgatc aactccctgt ccgtgtacgc acagactaac gaatacggct
     6481 tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa attcactatc
     6541 tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag
     6601 aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc agcttgttca
     6661 gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt
     6721 ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaacatgc
     6781 aacgtcaggc cgttccgact ctgcgtgctg ataagccgct ggttggtacg ggtatggaac
     6841 gtgctgttgc cgttgactcc ggtgtaactg cggttgctaa acgtggtggt gtcgttcagt
     6901 acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag
     6961 caggtatcga catctacaac ctgaccaagt acacccgttc taaccagaac acctgtatca
     7021 accagatgcc gtgtgtgtct ctgggtgaac cggtagaacg cggcgacgtg ctggcagacg
     7081 gtccgtccac cgatctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc
     7141 cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag
     7201 accgtttcac caccatccat attcaggaac tggcgtgtgt gtcccgtgat accaagctgg
     7261 ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgctctc tccaaactgg
     7321 atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt ctggttggta
     7381 aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct
     7441 tcggtgagaa agcgtctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg
     7501 gtacggttat cgatgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc
     7561 tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga
     7621 tcctcgaagc aggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag
     7681 ctgagaaact cgacaaactg ccgcgcgatc gctggctgga gctgggcctg accgacgaag
     7741 agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg
     7801 agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg
     7861 tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga
     7921 tggcaggccg tcatggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc
     7981 cttacgatga aaacggtact ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc
     8041 gtatgaacat tggtcagatc ctcgaaaccc acctaggtat ggctgcgaaa ggtatcggcg
     8101 acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc
     8161 agcgtgcgta cgatctgggc gctgatgttc gtcagaaagt tgacctgagt accttcagcg
     8221 atgaagaagt tatgcgtttg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg
     8281 tcttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga
     8341 cttctggtca gatccgcctg tacgacggtc gcactggtga acagttcgag cgtccggtaa
     8401 ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc
     8461 gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg
     8521 gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatatggc gcagcataca
     8581 ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtact aagatgtata
     8641 aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat
     8701 tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc
     8761 tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg
     8821 agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt
     8881 gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa
     8941 gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc
     9001 tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc
     9061 ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca gactaaagta
     9121 cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg
     9181 aaatcgctgc cgtcccgtat cggcctgctg ctcgatatgc cgctgcgcga tatcgaacgc
     9241 gtactgtact tcgaatccta tgtggttatc gaaggcggta tgaccaacct ggaacgtcag
     9301 cagatcttga ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac
     9361 gcgaagatgg gggcggaagc aatccaggcc ctgctgaaga gcatggatct ggagcaagag
     9421 tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg
     9481 accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg
     9541 atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt
     9601 ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac
     9661 cgtctgaaac gactactgga cctggctgcg ccggatatca tcgtacgtaa cgaaaaacgt
     9721 atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcaatcacc
     9781 ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt
     9841 ttccgtcaga acctgctcgg taagcgcgtt gactactccg gtcgttctgt aatcaccgta
     9901 ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc
     9961 aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat caaagctgcg
    10021 aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga agtgatccgc
    10081 gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt
    10141 gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat
    10201 aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag
    10261 ctcgaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa
    10321 ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgcgactgt
    10381 gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat
    10441 cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa
    10501 gatgctaacg gtgaattagt ggcgaaaacc agcctgaaag acacgactgt tggccgtgcc
    10561 attctgtgga tgattgtacc gaaaggtctg ccttacacca tcgtcaacca ggcgctgggt
    10621 aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgact
    10681 gttatttttg cggaccagat catgtatacc ggctttgcat atgcagcgcg ttctggtgca
    10741 tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca
    10801 gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa
    10861 cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg
    10921 atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt
    10981 tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt
    11041 cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat catcgaaacg
    11101 ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac
    11161 ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact
    11221 cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtactcat
    11281 gaaggtatca tgatgactcc ggttatcgaa ggtggtgacg ttaaagagcc gctgcgcgat
    11341 cgcgtattgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatatcctc
    11401 gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc
    11461 gacgcggtta aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac
    11521 tgctacggtc gtgacctggc gcgtggccac atcattaaca aaggtgaagc aatcggtgtt
    11581 atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc
    11641 ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc
    11701 atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc
    11761 cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagta
    11821 ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt
    11881 gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt
    11941 actgacatga tcgacggcca gaccattact cgtcagaccg acgaattgac aggtctgtct
    12001 tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca
    12061 ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc cgggtaccga tatgcctgcg
    12121 cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct
    12181 ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt
    12241 ctgccgcgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc aatcctggct
    12301 gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc
    12361 accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac
    12421 gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg
    12481 cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta
    12541 caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga agttatcgtt
    12601 cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggt
    12661 gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa
    12721 gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag
    12781 tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt
    12841 gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc
    12901 ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa
    12961 gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg
    13021 aacgcaggtc tgggtggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
    13081 ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaagtcttca cggaaaatat
    13141 taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat tcctgctgac
    13201 ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat cttccgtagc
    13261 ggctcggaat aaatttacgt tcttacatac tgtctcatgg cctttgcgat gggcgtccca
    13321 gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac gcggcagcaa
    13381 ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc cagctcgggg tggttgtcgg
    13441 catagccacc tggctgcgtt ttcgaaaaag cgctgacgtt attaattgcc agcggaatca
    13501 cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc tccggtgcaa
    13561 gcagccggaa agcgcagatg gcttgcacta actggcgttc atccataatc gacgcaggct
    13621 caatgccgcc agtacatgga cgcaggcgtg ggaaggagac agagtagcgg ctttgccagt
    13681 aatgctgttg cagccatagc aaatgttcga caaccatata gcagtcaact cgccagctgt
    13741 cggaaaggcc gattagcgcg ccgaggccta tcttatcaat ccccgcacgc cctagccgat
    13801 ccggcgtttc cagccgccag aagaagtcct gttttttacc tttcagatga tgatgggcat
    13861 aagtcgcctc atgatatgtc tcctgataaa ccattactcc atcgaggcca agctgtttta
    13921 actcggcata ttccgcctcc gccagcggtt gcacttccat ctgtagtgaa gagaactgtt
    13981 cacgcagggc agggagatga cgacgaaagt aatccatccc cactttcgcc tgatgttcac
    14041 cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt tctctggcaa
    14101 tatccgcttc atccagcgtt ttgcgcttga tgcggttgct catagaaaat ccgcaataag
    14161 tacagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaagctg acgacgttgc
    14221 caaatcgctg acgagtcaga cgttgcgctc gctgggccag tggttccaga tagccactgg
    14281 cggcgggtga tagcaatgcc atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc
    14341 gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcatcc cagtccagtt
    14401 gtcgccagcg atcgctgaag gttttcattc cgatgcctcc agaaatccag tcagcgggct
    14461 ggtggcatga gcaaaatgac tgcggctgcc cggtccggac tgacgtgcca gcaggcctgc
    14521 ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga cggcaatcgc
    14581 cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgcggcat ggctgggaac
    14641 gccgatgcca gcatcgacaa ccaccggaac ggtggcctgc tggataataa tctccagcat
    14701 ggcgcgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc
    14761 acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt aaggcagcac
    14821 gacaaatccc tgttgtacca gcttttcggc ggctttcagg gtttcgatgg gatcgggcaa
    14881 cagccagcgg gcgtcagggt gaatctctaa ttttaaccag tttgtgccta acgcttcacg
    14941 agccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggatg tatttggcag
    15001 cagggtcaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct ggcgcaagtt
    15061 gacgcgtttc atcgccagtg tcaccagttg gctgccgcaa gcgcggatcg cctccatcat
    15121 cagttgtgaa gaggcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc
    15181 cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc tggtcgccat
    15241 cctgcacgat atgttgcgcc cactgctcac gcgggacgat ttgctgatta atcgccagtg
    15301 ccgcgcccgc ttgttgttgg tcgagttgct ccagtagttc gtgaaccgtt tgcccggcgg
    15361 cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca taccgggcaa
    15421 ccactggcgc ggcgcaaagc caggctgcgc cactggctcg acttaccgtc gaacagtcgg
    15481 agttctcccg caggcgtctc tataccactt aataacttaa tcgcttccag tgcctgtaat
    15541 gtgcccataa ccccgaccac cgggccaacc acgcccgccg tgcggcagtt gcgttctggc
    15601 tcctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg cgtcagtacc
    15661 atcaactgac cgccaaatct gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg
    15721 caggtggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag caccacatcg
    15781 gcccgtgcta ccgcatcttt taacgcctca cccgtcaacc gttgttgtaa tgccatcagt
    15841 tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgtgattt cgggcgatcg
    15901 atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca
    15961 tctgccagta ccagcgtccc gacgccagcc cccgccaggt atagcgcagc aggtgtaccc
    16021 agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg cccgtccaga
    16081 gcgatatcgt cgagcaggat ttggcggcta taacgcataa agtcacggtc attcatcgcc
    16141 aactcctgca atttccagca gctgtgccgt tgccaaacgc cagtctgcgg cttgagtaat
    16201 ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg cgcgcgccag
    16261 actgataccg ccaatcgcca cggtgggata atccgctagg cgctcaacat gccgtgccag
    16321 ctgttccagt ccctgcggtg cagaaggcat ctgtttggtt tgtgtcggaa acacatgtcc
    16381 cagcgcgata taagaaggac gtgctgccag cgcgacgtcg atctccatat cgtcatgggt
    16441 cgaaacgccc agccgcaggc ctgccgcgcg gatggcgctg agatcggtgg cttgcaaatc
    16501 ttcctgcccc agatggacgc catacgcctg atgcttaatc gccagccgcc agtaatcgtt
    16561 gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacca catcggcttc
    16621 tacctcttca tcgcgctgat ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa
    16681 cagacgttcg atccactgta cgctgtcaac caccgggtac agtcctaaac gaaagggtac
    16741 aggaggaaac tcaggctgat acatcacgct tcctccttac gcaggtagat ttctccgcct
    16801 ctggcgcgga agttctccga catatccgcc attcccactt caatggtttg cgcggcggcg
    16861 taatcacgca cttcctggct gattttcatc gagcagaatt tcggtccaca catggagcaa
    16921 aaatgggcga ctttaccgga ctcttgcggc agggtttcat cgtgataagc gcgggcagta
    16981 aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg ggctttcgac
    17041 atggcgttat cgcgaatttg cgcgcccgga tgccctttcg ccaggtcggc ggcgtgagcg
    17101 gcaatcttat aggtgataat cccttgttta acatcttctt tatttggcag gcccagatgc
    17161 tctttcggcg ttacgtagca gagcatcgcg cagccaaacc agccaatcat cgccgcgcca
    17221 atccctgacg tgaagtggtc atagcccggc gcgatatcgg tagttagcgg ccccagagtg
    17281 taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg aatcatctgc
    17341 atcggcacgt ggcctgggcc ttcaatcatc acctgcacgt catattccca ggcgatttta
    17401 gtcagttcac ccagcgtatg cagctcggca aactgtgctt catcgttggc gtcctgaaca
    17461 gaaccggggc gcaggccgtc gcccagcgat agagaaacgt cataagcagc acagatttca
    17521 caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag gcaccatttc
    17581 gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt catcggcacg
    17641 tagcgcagca gcacccccgc atggatagtg aagtaatcca caccttgctc ggcctgttcc
    17701 agcagggtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt caccttctcc
    17761 agcgcctggt agatcggcac tgtaccgatc ggcaccgggc tgttacgcaa aatccactcg
    17821 cgggtttcgt gaatatagcg accagtggaa agatccatca cggtatccgc tccccagcgc
    17881 gtggaccata ccagtttttc cacttcttct tcgatggaag aagtgaccgc cgagttgccg
    17941 atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc cgattccgga
    18001 tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc
    18061 gcagtgatat tttccggcag acgtgcgcca aagctcattc ccggatgctg gtggcgtaaa
    18121 acttcgctac gtatgcgctc gcgacccata ttctcgcgaa tggcgatgaa ttccatttct
    18181 ggcgtgatga tacccttgcg ggcgtagtgc agttgggtga cgcggcgtcc tgctttggcg
    18241 cgttttggcg ttaatacgcc gctaaaacgc agctcgtcga gaccatcatc tgccagccgc
    18301 gcttttgtgt aatcggaact gcgcacggta agttcttcgg tatcgccgcg cgcatcgatc
    18361 cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg cggatcgcca
    18421 tacggaccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg cggctgttct
    18481 ttgctgccgc cgattagcgt cgggctaagc tggatctcac gcattggcac gcgcacgccg
    18541 ggttgtgtgc cagtgatgta aatgcgtttt gagttgggaa aggcggagcc ttccagggtg
    18601 tcgataaaat gttgggcctg ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata
    18661 gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca agagacgatc
    18721 gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta ttagcctgat
    18781 caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg acaagaaatt
    18841 agccccacaa acggggcgtt gaatgtaaat tacgcgttaa cagcgcagaa ctcaagctgg
    18901 atatttgacg cgggcggcgt caagcaccag tagaatcagc ttatcttcca gcacaaagcg
    18961 cgcttccagt gcctcgccga tgtcagataa aacctgttga aactcaaggt aattatcatg
    19021 atcgatggcg gtttccagac tggaatcgta gtcatccata atctgttgag tattggcttc
    19081 gagttgtggc caaatcttcg cggcgcgtgc gagttgacca ttgccttcca gcttatgaag
    19141 aatgcgctca taaatactga aatgtccggc agacaagtaa tcgaccaggc tctgacaaaa
    19201 atcatcaagg gctttttcgt ttagcctcat gtacgattct ttgccaggct taacgccaac
    19261 cagattgtag taagccacga gcagatgctt acgtacatgt agccagcgat caaccagttt
    19321 gttacttcct ctgacgcgct ccgtcaggtt atcgagctgg ttaagcatga ttgactccgc
    19381 aagtttgtat tcaaaaactg ctctgtgaga aatgtaaaaa ccatgttaaa catgccagtg
    19441 atgcaaaggt agtgcaagag ctatggatcg tataattgaa aaattagatc acggctggtg
    19501 ggtcgtcagc catgaacaaa aattatggtt gccgaaggga gaattgccat atggcgaagc
    19561 ggcaaatttc gatcttgtgg gtcagcgcgc actacaaatc ggcgaatggc agggggaacc
    19621 tgtttggtta gtacaactgc agcggcgtca cgatatgggg tcggtacgtc aggtcattga
    19681 tctcgatgtt gggctgtttc aactggccgg acgtggcgtg caactggcgg agttttaccg
    19741 atcgcataaa tactgtggtt actgcgggca tgaaatgtat ccaagcaaaa ccgaatgggc
    19801 gatgctgtgc agccattgcc gtgagcgtta ctacccgcaa atcgccccct gcattattgt
    19861 tgccatccgt cgcgatgatt cgatcctcct cgcccagcat acccgccatc gtaacggtgt
    19921 ccatacagta cttgccggat tcgtcgaagt gggtgaaacc ctcgagcagg cagtcgcgcg
    19981 ggaagtgatg gaagagagcg gaattaaagt taaaaacttg cgttacgtga cctcccaacc
    20041 gtggccgttt cctcagtcgt taatgaccgc gtttatggcg gaatatgaca gcggcgagat
    20101 cgtgatcgac ccgaaagaat tgcttgaggc gaactggtat cgctatgacg atttgccgtt
    20161 actcccgccg cccggcaccg tagcgcgccg tctgatagaa gatacagtgg cgatgtgtcg
    20221 ggcagagtat gagtgatgat acactgaccg cctgacgcac taaggaacag ccaaaatgac
    20281 cgaacttaaa aacgatcgtt atctgcgggc gctgctgcgc cagcccgttg atgtcactcc
    20341 agtatggatg atgcgccagg cgggtcgcta tctaccggaa tataaagcca cgcgcgccca
    20401 ggcgggcgat tttatgtcgc tgtgcaaaaa tgccgagctg gcgtgcgaag tgactttgca
    20461 accgctgcgt cgctatccgc tggatgcggc gatcctcttt tccgatatcc tcaccgtgcc
    20521 ggacgcgatg gggttagggc tctattttga agccggagaa ggtccgcgtt ttacctcgcc
    20581 agtcacctgc aaagccgacg tcgataaact gccaattccg gacccggaag atgagctggg
    20641 ttacgtgatg aacgcggtgc gtaccattcg tcgcgaactg aaaggcgaag tgccgctgat
    20701 tggtttttcc ggcagcccgt ggacgctggc gacttacatg gttgaaggcg gtagcagcaa
    20761 agccttcacc gtgattaaaa agatgatgta tgccgatccg caggcgctgc acgcgctgct
    20821 cgataaactg gcgaaaagcg tcacgttgta tctgaatgcg cagattaaag ccggtgctca
    20881 ggcagtcatg attttcgaca cctggggcgg cgtgcttacc gggcgcgatt atcaacagtt
    20941 ctcgctttat tacatgcata aaattgttga tggtttactg cgtgaaaacg acggtcgccg
    21001 cgtgccagtc acgctgttta ccaaaggtgg cggacagtgg ctggaagcga tggcagaaac
    21061 cggttgcgat gcgctgggcc tcgactggac aacggacatc gccgatgcgc gccgtcgcgt
    21121 gggcaataaa gtcgcgctgc agggtaatat ggacccgtcg atgctgtacg cgccgcctgc
    21181 ccgcattgaa gaagaagtgg cgtctatact tgccggtttc ggtcacggcg aaggccatgt
    21241 ctttaacctt ggtcacggca ttcatcagga tgtgccgcca gaacatgcag gcgtgtttgt
    21301 ggaggcagtg catcgcttgt ctgaacagta tcaccgctaa ggagcgatta tggatctcgc
    21361 gtcattacgc gctcaacaaa ttgaactggc ttcttctgtg atccgcgagg atcgactcga
    21421 taaagatccg ccggatctga tcgccggagc cgatgtcgga tttgagcagg gcggagaagt
    21481 gacgcgagcg gcgatggtgc tgctgaaata cccctcgctt gagctggtgg agtataaagt
    21541 ggcccgcatc gccaccacca tgccttacat tccaggtttt ctctcgttcc gcgaatatcc
    21601 tgcgctgctg gcagcgtggg agatgctgtc gcaaaagccg gatttagtgt ttgtcgatgg
    21661 tcatgggatc tcgcatcctc gccgtcttgg cgtagccagc cattttggct tattggtgga
    21721 tgtgccgacc attggcgtag cgaaaaaacg gctctgcggt aaattcgaac cgctctccag
    21781 cgaaccgggc gcgctggccc cgctgatgga taaaggcgag cagctggcct gggtctggcg
    21841 cagcaaagcg cgctgtaacc cgttgtttat cgctaccggc catcgggtca gcgtggacag
    21901 cgcgctggcg tgggtacaac gctgcatgaa aggctatcgt ctgccggagc caacgcgctg
    21961 ggcggacgcg gtggcctcgg aacgtccggc gttcgtgcgc tatacagcaa atcagcccta
    22021 attcaggtaa actgcggata atttccgtat ttgagaactc aacatgttac aaaacccaat
    22081 tcatctgcgt ctggagcgcc tggaaagctg gcagcatgtc acttttatgg cctgcttatg
    22141 cgaacgcatg taccccaatt acgccgtatt ctgtcagcaa accggcttcg gcgatgggca
    22201 gatttaccgc cgcattctcg atctcatttg ggaaacgctg accgtcaaag acgcaaaagt
    22261 aaatttcgac agccaactgg agaaatttga agaagcgatt ccttcagccg acgatttcga
    22321 tctgtacggc gtttatccgg caattgatgc ttgcgtggcg ttaagtgaac tggtccattc
    22381 gcgtttaagt ggcgaaacgc tcgaacacgc ggtggaagtg agtaagacct ccatcacgac
    22441 cgtggcgatg ctggaaatga ctcaggctgg tcgcgaaatg agcgatgaag agctcaaaga
    22501 aaacccagct gtagagcaag aatgggacat tcagtgggaa atattccgac ttttagctga
    22561 gtgcgaagaa cgcgatatcg agctgataaa aggccttagg gcagacctgc gtgaggcggg
    22621 tgagagcaat attggtataa tttttcagca ataagaccag aaaacgtgat ttaacgcctg
    22681 atttgtcgta cctggagtct tccctttcgc cccccgtctg gtctacattt ggggggcgaa
    22741 aaaaagtggc tatcggtgcg tgtatgcagg agagtgctat tctggcattt ccgtcgcact
    22801 cgatgcttag caagcgataa acacattgta aggataactt atgaacaaga ctcaactgat
    22861 tgatgtaatt gcagagaaag cagaactgtc caaaacccag gctaaagctg ctctggagtc
    22921 cactctggct gcaattactg agtctctgaa agaaggcgat gctgtacaac tggttggttt
    22981 cggtaccttc aaagtgaacc accgcgctga gcgtactggc cgcaacccgc agaccggtaa
    23041 agaaatcaaa attgccgcag ctaacgtacc ggcatttgtt tctggcaagg cactgaaaga
    23101 cgcagttaag taagattgcg tggcagtgaa cagttttaac gaaggggtgg tttcacccct
    23161 tttgtctttc tggcgtcgat cattgatgct ggcaggcgct ctgtttctca ctgcctgtag
    23221 tcacaactct tcacttcctc cctttaccgc cagtggattt gctgaagacc agggcgcggt
    23281 acgcatctgg cgaaaagaca gcggcgataa tgtgcatctg cttgccgtgt ttagcccgtg
    23341 gcgcagtggc gataccacga cgcgagagta tcgctggcag ggcgataacc ttacgctcat
    23401 caatatcaat gtttacagca aaccgccggt gaacattcgc gcgcgttttg acgatcgcgg
    23461 cgatctgagc tttatgcaac gtgaatccga tggggaaaag cagcagcttt ctaacgacca
    23521 aatcgattta taccgttatc gtgctgatca gatccgccag attagcgatg ccttacgtca
    23581 ggggagagtc gtgctgcgcc aggggcgctg gcatgcgatg gaacagaccg tgaccacctg
    23641 cgaagggcaa accattaaac ctgatttaga ttcgcaggcg atagcgcata tcgagcgccg
    23701 ccagagccgc tcttctgttg atgtcagcgt ggcatggctg gaagcgcccg aaggttcgca
    23761 attactgttg gtggcaaact ctgatttctg tcgctggcaa cccaacgaga aaacgttctg
    23821 atttaccagt ggcccatacc catatgaccg ccaccaccgc agtcgccgta acccattccg
    23881 gtaccgcgcg gaatacccgc ttcagccatc gcaatatctc gtttcacccg taactcatct
    23941 aacgactgac gcaaattctc catctctttg gcgaccgcgt taattttgct gctatccggt
    24001 gggttcgcgg ctaacagggc attgtattca taacgctttg tcaccagttg ctgttgcagt
    24061 gcgctgcttt gagcgtaaaa gtcattatgg attttctgcc acgctgtctg ttgttcgctg
    24121 gtcaaaggcg cggcattttg ctgccacata ccatgtccgc cgtgagcaaa tgcagatgtc
    24181 gatcccatcg ccattgctga aagcgccatc attaccaggg caattttcgt gttccgtttc
    24241 atggttaatc ctccagtggt tgtcttactt cgggtattgc atcttccgtg ccaacggcga
    24301 aacgctgata tgacgggtaa tctggcatga taaacgagta aaaatgactc gcctgctgcg
    24361 ggtagcgagt catttttact cattgaaact taagcctttg tgttacagct cagggtaagc
    24421 gctgataaaa gatggcatga tttctgctgt cagaaaggga tggacaggca aagaagaaga
    24481 tgcgttttat gcaacgttct aaagactcct tagctaaatg gttaagcgcg atcctccccg
    24541 tggtcattgt tgggctggtg gggctgtttg cggtgacagt gattcgcgat tatgggcgcg
    24601 agactgccgc cgccagacaa acgctgctgg aaaaaggcag tgtacttatt cgtgctcttg
    24661 aatccggctc gcgcgtcggc atggggatgc gcatgcatca tgcgcagcag caggccttac
    24721 ttgaagaaat ggccgggcag cctggagtac gttggtttgc ggtcacggat gaacaaggaa
    24781 caatcgtgat gcatagcaac tccggcatgg tgggaaaaca gctttattcc ccgcaggaaa
    24841 tgcagcagtt acatccggga aatgaagaag cgtggcggcg gatcgatagc gcagacggcg
    24901 agcctgttct ggaaatttat cgccagtttc aaccgatgtt tgctactgga atgcaccgga
    24961 tgcgccatat gcagcagtat gccgcgacac cacaagcaat ttttatcgct ttcgacgcca
    25021 gtaacattgt gagtgccgaa gatcgtgagc agagaaacac cctgattatc ctcttcgccc
    25081 tggcgacggt cttgctggca agcgtgttgt cattcttctg gtatcgccga tatctgcgct
    25141 cgcggcagct attgcaggat gaaatgaagc gcaaagagaa gctggtggca ctggggcatc
    25201 tggcggcagg cgttgcccac gaaatccgta atccactttc ctcgattaaa gggctggcga
    25261 aatactttgc cgaacgcgcg tcagcagggg gagaagcgca tcaattggcg caggtgatgg
    25321 cgaaagaagc cgaccgttta aaccgcgtgg taagcgagct gctggaactg gttaagccaa
    25381 cgcatctggc tttgcaggcg gtggagctca acacgctgat tagccactca ttacagctgg
    25441 taagccagga tgcaaacagc cgggagatcc agttacgctt taccgccaac gacacattac
    25501 cggaaattca ggccgatccg gacaggctga ctcaggtcct gttgaatctc tatctcaacg
    25561 ctattcaggc gattggtcag catggcgtga ttagcgtgac ggccagcgaa agcggcgcgg
    25621 gcgtgaaaat cagcgttacc gacagcggta agggaattgc ggcggatcag cttgaagcca
    25681 tcttcactcc gtacttcacc accaaagccg aaggcaccgg actggggctg gcggttgtgc
    25741 ataatattgt tgaacaacac ggtggtacaa ttcaggtcgc aagccaggag ggaaaaggcg
    25801 caacgttcac cctttggctt ccggtcaata ttacgcgtaa ggacccacaa ggatgacgca
    25861 cgataatatc gatattctgg tggtggatga tgacattagc cactgcacta ttttgcaggc
    25921 tttgctgcgc ggctggggct ataacgtcgc gctggcgaac agcgggcgac aggcgctgga
    25981 gcaggtgcgg gaacaggttt ttgatcttgt gctttgcgat gtgcgaatgg cagagatgga
    26041 tggtatcgcc acgctaaaag agatcaaaac attaaacccc gcgattccgg tgctgattat
    26101 gaccgcgtac tccagcgtcg agacggcggt agaggcgttg aaaaccgggg cgctggatta
    26161 tctcatcaag ccgctggatt tcgataacct acaggcgacg ctggaaaagg cgctcgcgca
    26221 tacacacagt gttgacgctg aaacgcctgc ggtgtctgcc agccagttcg gtatggtcgg
    26281 taaaagcccg gcgatgcaac acctgctcag tgaaatcgcc ctcgtcgcgc catcggaagc
    26341 cacggtgctg atccacggcg attccggcac cggtaaagag ctggtcgcca gggcgattca
    26401 cgccagtagc gcacgtagcg aaaaaccgct ggtaacgctc aactgcgcgg cgctcaacga
    26461 atccttgctg gagtctgaat tgttcggtca cgaaaaaggg gcgtttaccg gggccgacaa
    26521 acgccgggaa gggcgctttg ttgaggcgga cggcggcacg ttgtttctcg atgaaattgg
    26581 cgatatctcg ccgatgatgc aggtgcgtct gctacgtgcg attcaggagc gcgaagttca
    26641 gcgtgtcggt agcaaccaga ctatttcggt tgatgtccgg ctgattgcgg cgacccatcg
    26701 cgatcttgcc gcagaggtga atgccgggcg ttttcgccag gatctctact atcggctgaa
    26761 cgtggtggcg attgaaatac catcgctgcg gcaacggcgg gaagatattc ctctgctggc
    26821 tggccatttt cttcagcgct ttgccgaacg taatcgcaag gcggtaaaag gctttacgcc
    26881 ccaggcgatg gatctgctga ttcattacga ctggccggga aatattcgtg aactggaaaa
    26941 cgcggtggaa cgggcggtgg tgctgctgac cggggaatat atttccgaac gcgagctgcc
    27001 gctggcgatt gccagtacgc cgatcccgct ggtacaaagt caggatattc agccgctggt
    27061 ggaagtcgaa aaagaagtga ttctggcagc actggagaaa acgggcggca acaaaaccga
    27121 agccgcccgt cagttaggga tcacgcgtaa aacgctattg gcaaaactgt cgcgttagtt
    27181 ctgctcgcgt tcgatagcgc gccagccgat atctttccgg cagaagcagt cgtcccaatg
    27241 gatatcggtc attaaggcat aagcgcgttt ctgtgcttct gccacggtat gacccagcgc
    27301 ggtaacgcac agtacgcgcc cgccgctggt cactacctgc tcgtcatccg ccagtttcgt
    27361 gcccgcgtgg aacactttgc cgtctgccac ttcttccagc ggcaggccgt ggatcacatc
    27421 gccggtgcgg taatcgcccg gatatccacc cgcagccatc accacgccga gagaagcgcg
    27481 ttcatcccac tcggacgttt tctcgtccag cttgccttca caggccgcca ggcagagctc
    27541 aaccagatcg gacttcatgc gcagcataat cggctgggtt tccggatcgc caaagcggca
    27601 gttaaattca ataaccttcg gattgccctg tttgtcgatc atcaggcccg cgtagagaaa
    27661 accagtgtag gtgttgcctt ccgccgccat gcctttcacg gttggccaga tgatacgttc
    27721 catcgtgcgc tgatgaacct catcggtcac tactggcgca ggggagtaag cgcccatccc
    27781 gccggtgttc ggcccggtat ctttatcgcc tacgcgtttg tgatcctggc tggtggccat
    27841 cggcagcaca tgctcgccgt ccaccatcac gataaagctc gcttcttcgc cgtcgaggaa
    27901 ctcttcgata acaatgcgat gacccgcgtc gccaaacgcg ttgcccgcca gcatgtcgcg
    27961 cacggcggct tcagcttctt ccagcgtcat cgccacgata acgcctttcc cggcggccag
    28021 accgtccgct ttaatgacga ttggcgcgcc tttctcacgc agatacgcca gtgcaggttc
    28081 tacctcggtg aagttctggt attccgctgt gggaatgtta tggcgggcca gaaagtcttt
    28141 ggtgaatgct ttagaacctt ccagttgtgc ggcacctgcg gttgggccga agattttcag
    28201 cccggcggcg cggaaggtat cgactacgcc tttcaccagc ggcgcttccg ggccgacgat
    28261 ggttagatct accttttcgt tttgtgcgaa atccagcagc gccgggatat cggttacgcc
    28321 gatagcgacg ttctgtagcg cgggttccag cgcagtgcct gcattacccg gagcaacaaa
    28381 aacagtttca accagcggcg actgggccgc tttccaggcc agcgcgtgct cgcgcccgcc
    28441 gttaccgatc actaatactt tcatctgtcg ctccattaat ggcggaagtg gcgcatgtcg
    28501 gtgaagagca tcgcaatacc gtgctcgtcg gcggcggcaa ttacttcgtc atcacggata
    28561 gaaccgccag gctggattac gcaggtcacg cccgcagcgg ctgcggcatc aataccgtcg
    28621 cggaacggga agaacgcgtc agaagccatc gaggaacctt tcacttccag accttcatcg
    28681 gcagctttga taccggcgat tttcgcggag tacacgcggc tcatctggcc tgcgccaata
    28741 ccgatggtca tattgttttt ggcatagacg atagcgttgg atttcacgaa cttcgccact
    28801 ttccagcaga acagcgcatc acgaagttcc tgttcagtcg gctgacgttt ggtgacgacg
    28861 cgcagttctt ccgcaccgac catccccaga tcgcgatcct gaaccagcag accgccgttc
    28921 acgcgtttga aatcaagacc cggaatacgt tcgccccact gaccgcaggt cagaacgcgt
    28981 acgttctgct tggcggcggt gattttcagg gcttcttcgc tggcggacgg cgcaataatc
    29041 acttcgacga actgacgaga aatgatggcc tgcgcggttt cagcatccag ttcgcggtta
    29101 aaggcaatga tgccgccgaa tgcggaggtt gggtcggttt tgtacgcgcg atcgtaagca
    29161 tcaagaatgg aattgccgat agccacgccg caagggttgg cgtgtttcac aatcacacat
    29221 gccggctcgg cgaactcttt cacgcactcc agcgccgcat cggtatcggc gatgttgtta
    29281 taagagaggg ctttaccctg aacctgggtt gcggtggcaa cggaggcttc tttcacattc
    29341 tcttctatat agaaggcagc ctgctggtgg ctgttttcgc cgtaacgcat atcctgcttc
    29401 ttaatgaagt tcaggttcag cgtgcgtggg aagcgaccgg cggcttcttt gttttcaccg
    29461 tggtaagccg gaaccatgct gccgaagtag ttggcaatca tgctgtcgta ggcggcggtg
    29521 tgttcgaagg ctttgatggc aaggtcaaag cgagtttcca gagtcaggga gccttcattg
    29581 gcatctatct ctttaataat ggcgtcgtag tcgctgctct tcaccacgat ggcgacatct
    29641 ttatggttct tggcggcaga gcgcaccatc gttgggccgc cgatatcgat gttctcaacc
    29701 gcatcttcca gcgagcaacc ttcacgggca acggtctggg cgaacggata cagattaaca
    29761 accaccatat cgataggctg gatctgatgt tcttccataa tggcatcgtc ctggtcgcga
    29821 cggcccagaa tgccaccatg tactttcgga tgcagggtct tcacgcgtcc atccatcatc
    29881 tccgggaaac cggtgtaatc ggaaacttcg gttaccggca gacctttttc tgctaacaga
    29941 cgggctgtgc cccctgtaga cagcagctcc acaccgcgtg cggaaagtgc ctgggcgaat
    30001 tcgacgatac cggctttgtc agaaacactg agcagagcgc ggcggactgg acgacgttgt
    30061 tgcatggtaa atcccctgga tttgactatt acagagagcg ttagctgaat ttttcgcgaa
    30121 aaactcagct aacgccccta acggggcatc cttatttttc gcccgcattg taacgaaaac
    30181 gtttgcgcaa cgctcgcgaa tttttctctt tcaatggtga tcacaatttt gacagtggct
    30241 accgtgggaa aaatacagaa attacattga agattgtgga taactctgtg cgtaaaaagg
    30301 tataaagcgg ggttttgctg gggaatgcag cagtcagtca tttttatgca atttttctat
    30361 tgcggcctgc ggagaactcc ctataatgcg cctccatcga cacggcggat gtgaatcact
    30421 tcacacaaac agccggttcg gttgaagaga aaaattctga a
//
LOCUS       DAZGJT010000052        29964 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZGJT010000052 DAZGJT010000000
VERSION     DAZGJT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.052"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            145..1713
                     /gene="cydA"
                     /locus_tag="ACHKFK_002581"
     CDS             145..1713
                     /gene="cydA"
                     /locus_tag="ACHKFK_002581"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO9052894.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1729..2868
                     /gene="cydB"
                     /locus_tag="ACHKFK_002582"
     CDS             1729..2868
                     /gene="cydB"
                     /locus_tag="ACHKFK_002582"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO9052895.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            2883..2996
                     /gene="cydX"
                     /locus_tag="ACHKFK_002583"
     CDS             2883..2996
                     /gene="cydX"
                     /locus_tag="ACHKFK_002583"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO9052896.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            2996..3289
                     /gene="ybgE"
                     /locus_tag="ACHKFK_002584"
     CDS             2996..3289
                     /gene="ybgE"
                     /locus_tag="ACHKFK_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO9052897.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3439..3843
                     /gene="ybgC"
                     /locus_tag="ACHKFK_002585"
     CDS             3439..3843
                     /gene="ybgC"
                     /locus_tag="ACHKFK_002585"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9052898.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            3840..4532
                     /gene="tolQ"
                     /locus_tag="ACHKFK_002586"
     CDS             3840..4532
                     /gene="tolQ"
                     /locus_tag="ACHKFK_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO9052899.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLSGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4536..4964
                     /gene="tolR"
                     /locus_tag="ACHKFK_002587"
     CDS             4536..4964
                     /gene="tolR"
                     /locus_tag="ACHKFK_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO9052900.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5029..6294
                     /gene="tolA"
                     /locus_tag="ACHKFK_002588"
     CDS             5029..6294
                     /gene="tolA"
                     /locus_tag="ACHKFK_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO9052901.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKNQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAVEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6427..7719
                     /gene="tolB"
                     /locus_tag="ACHKFK_002589"
     CDS             6427..7719
                     /gene="tolB"
                     /locus_tag="ACHKFK_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO9052902.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7754..8275
                     /gene="pal"
                     /locus_tag="ACHKFK_002590"
     CDS             7754..8275
                     /gene="pal"
                     /locus_tag="ACHKFK_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO9052903.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYAKNRRAVLVY"
     gene            8285..9076
                     /gene="cpoB"
                     /locus_tag="ACHKFK_002591"
     CDS             8285..9076
                     /gene="cpoB"
                     /locus_tag="ACHKFK_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO9052904.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSGDQSGVAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9241..9316
                     /locus_tag="ACHKFK_002592"
     tRNA            9241..9316
                     /locus_tag="ACHKFK_002592"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9274..9276,aa:Lys,seq:ttt)
     gene            9352..9427
                     /locus_tag="ACHKFK_002593"
     tRNA            9352..9427
                     /locus_tag="ACHKFK_002593"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9385..9387,aa:Val,seq:tac)
     gene            9430..9505
                     /locus_tag="ACHKFK_002594"
     tRNA            9430..9505
                     /locus_tag="ACHKFK_002594"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9463..9465,aa:Lys,seq:ttt)
     gene            9557..9632
                     /locus_tag="ACHKFK_002595"
     tRNA            9557..9632
                     /locus_tag="ACHKFK_002595"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9590..9592,aa:Val,seq:tac)
     gene            9636..9711
                     /locus_tag="ACHKFK_002596"
     tRNA            9636..9711
                     /locus_tag="ACHKFK_002596"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9669..9671,aa:Lys,seq:ttt)
     gene            9760..9835
                     /locus_tag="ACHKFK_002597"
     tRNA            9760..9835
                     /locus_tag="ACHKFK_002597"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9793..9795,aa:Lys,seq:ttt)
     gene            9869..9944
                     /locus_tag="ACHKFK_002598"
     tRNA            9869..9944
                     /locus_tag="ACHKFK_002598"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9902..9904,aa:Lys,seq:ttt)
     gene            10222..11265
                     /gene="nadA"
                     /locus_tag="ACHKFK_002599"
     CDS             10222..11265
                     /gene="nadA"
                     /locus_tag="ACHKFK_002599"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO9052905.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGCYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVEMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALELEGSNHEVYVDERLLERALVP
                     LNRMLDFAATLRG"
     gene            11303..12022
                     /gene="pnuC"
                     /locus_tag="ACHKFK_002600"
     CDS             11303..12022
                     /gene="pnuC"
                     /locus_tag="ACHKFK_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO9052906.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVAMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12019..12960)
                     /gene="zitB"
                     /locus_tag="ACHKFK_002601"
     CDS             complement(12019..12960)
                     /gene="zitB"
                     /locus_tag="ACHKFK_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO9052907.1"
                     /translation="MAHSHSHASSHLPEDNNARRLLYAFGVTAGFMLVEVIGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRLCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13074..13454)
                     /gene="ybgS"
                     /locus_tag="ACHKFK_002602"
     CDS             complement(13074..13454)
                     /gene="ybgS"
                     /locus_tag="ACHKFK_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO9052908.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQSNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSDGTMLHPDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            13770..14822
                     /gene="aroG"
                     /locus_tag="ACHKFK_002603"
     CDS             13770..14822
                     /gene="aroG"
                     /locus_tag="ACHKFK_002603"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO9052909.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWDDT
                     DALLRQLANAVNARRG"
     gene            complement(14989..15741)
                     /gene="gpmA"
                     /locus_tag="ACHKFK_002604"
     CDS             complement(14989..15741)
                     /gene="gpmA"
                     /locus_tag="ACHKFK_002604"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO9052910.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(15944..16984)
                     /gene="galM"
                     /locus_tag="ACHKFK_002605"
     CDS             complement(15944..16984)
                     /gene="galM"
                     /locus_tag="ACHKFK_002605"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO9052911.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILAEEYLPVDEGGIPHDGLKSVAGT
                     SFDFRNAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAQ"
     gene            complement(16978..18126)
                     /gene="galK"
                     /locus_tag="ACHKFK_002606"
     CDS             complement(16978..18126)
                     /gene="galK"
                     /locus_tag="ACHKFK_002606"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO9052912.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALFPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18130..19176)
                     /gene="galT"
                     /locus_tag="ACHKFK_002607"
     CDS             complement(18130..19176)
                     /gene="galT"
                     /locus_tag="ACHKFK_002607"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO9052913.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGETYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAAQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19186..20202)
                     /gene="galE"
                     /locus_tag="ACHKFK_002608"
     CDS             complement(19186..20202)
                     /gene="galE"
                     /locus_tag="ACHKFK_002608"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO9052914.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKANRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20465..21937)
                     /gene="modF"
                     /locus_tag="ACHKFK_002609"
     CDS             complement(20465..21937)
                     /gene="modF"
                     /locus_tag="ACHKFK_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO9052915.1"
                     /translation="MSSLQILQGTFRLGDTKTLQLPRLTLNAGDSWAFVGSNGSGKSA
                     LARALSGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLSPGEDDTGRTT
                     AEIIQDEVKDTPRCMQLAQLFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAELLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSELLVGVQMPEPDEPSVRHALPAHEPRIVLNNGVVSYNERPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GKTQLLFVSHHAEDAPACITHRLEFVPDGDFYRYALTKIN"
     gene            complement(22005..22793)
                     /gene="modE"
                     /locus_tag="ACHKFK_002610"
     CDS             complement(22005..22793)
                     /gene="modE"
                     /locus_tag="ACHKFK_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO9052916.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AVTAQSGARLGLDEGKEVLILLKAPWVGITQDDAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTTYFNADSVIIATLC"
     gene            22922..23071
                     /gene="acrZ"
                     /locus_tag="ACHKFK_002611"
     CDS             22922..23071
                     /gene="acrZ"
                     /locus_tag="ACHKFK_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO9052917.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23238..24011
                     /gene="modA"
                     /locus_tag="ACHKFK_002612"
     CDS             23238..24011
                     /gene="modA"
                     /locus_tag="ACHKFK_002612"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9052918.1"
                     /translation="MARKWLNLFTGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24011..24700
                     /gene="modB"
                     /locus_tag="ACHKFK_002613"
     CDS             24011..24700
                     /gene="modB"
                     /locus_tag="ACHKFK_002613"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO9052919.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGLSFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDIKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            24703..25761
                     /gene="modC"
                     /locus_tag="ACHKFK_002614"
     CDS             24703..25761
                     /gene="modC"
                     /locus_tag="ACHKFK_002614"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO9052920.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLSPEKRRVGYVFQDARLFPHYKVRGNLRYGVA
                     KSMVNQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(25762..26580)
                     /gene="ybhA"
                     /locus_tag="ACHKFK_002615"
     CDS             complement(25762..26580)
                     /gene="ybhA"
                     /locus_tag="ACHKFK_002615"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO9052921.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYRLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQNFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            26735..27730
                     /gene="pgl"
                     /locus_tag="ACHKFK_002616"
     CDS             26735..27730
                     /gene="pgl"
                     /locus_tag="ACHKFK_002616"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO9052922.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(27771..28724)
                     /gene="ybhD"
                     /locus_tag="ACHKFK_002617"
     CDS             complement(27771..28724)
                     /gene="ybhD"
                     /locus_tag="ACHKFK_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9052923.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVQRQRHALQEIG"
     gene            28908..29960
                     /gene="ybhH"
                     /locus_tag="ACHKFK_002618"
     CDS             28908..29960
                     /gene="ybhH"
                     /locus_tag="ACHKFK_002618"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO9052924.1"
                     /translation="MEKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         7172 a         7326 c         7943 g         7523 t
ORIGIN      
        1 gtaacttttt tatcattgtc ttttcctctt ttgatgaaat ggcaacgata gaggcgagca
       61 aataattttg tattggggca tgcgtgtgac cctttctaac ggggttcact ctcggagtct
      121 tcatgcgatg agcaaggagt catgatgtta gatatagtcg aactgtcgcg cttacagttt
      181 gccttgaccg cgatgtacca cttccttttt gtgccactga cgctcggtat ggcgttcctg
      241 ctggccatta tggaaacggt ctacgtcctt tccggcaaac agatttataa agatatgacc
      301 aagttctggg gcaagttgtt tggtatcaac tttgctctgg gtgtggctac cggtctgacc
      361 atggagttcc agttcgggac taactggtct tactattccc actatgtagg ggatatcttc
      421 ggtgcgccgc tggcaatcga aggtctgatg gccttcttcc tcgaatccac ctttgtaggt
      481 ctgttcttct tcggttggga tcgtctgggt aaagttcagc atatgtgtgt cacctggctg
      541 gtggcgctcg gttcaaacct gtccgcactg tggattctgg ttgcaaacgg ctggatgcaa
      601 aacccaatcg cgtccgattt caactttgaa actatgcgta tggagatggt gagcttctcc
      661 gagctggtgc ttaacccggt tgctcaggtg aaattcgttc acaccgtagc ttctggttat
      721 gtgactggcg caatgttcat cctcggtatc agcgcatggt atatgctgaa aggtcgtgac
      781 ttcgccttcg ctaaacgctc ctttgctatc gctgccagct tcggtatggc tgccgttctg
      841 tctgttattg ttctgggtga tgaatccggc tacgaaatgg gcgacgtgca gaaaaccaaa
      901 ctggctgcta tcgaagccga gtgggaaacg caacctgcac ctgctgcctt tactctgttc
      961 ggcattcctg atcaggaaga ggagacgaac aaatttgcga tccagatccc ttacgcgctg
     1021 ggcatcattg caacgcgttc cgtggatact ccagttatcg gcctgaaaga actgatggtg
     1081 cagcatgaag aacgcattcg taacgggatg aaggcgtact ctctgctcga acaactgcgt
     1141 tctggttcta ccgaccaggc cgttcgtgac cagttcaata gcatgaagaa agatctcggt
     1201 tacggtctgc tgctgaaacg ctatacgcca aacgtggctg atgcgactga agctcagatt
     1261 caacaggcaa ccaaagactc cattccgcgt gtagcgccgc tgtacttcgc gttccgtatc
     1321 atggtggcgt gtggcttcct gctgctggca atcattgcgc tctctttctg gagtgtcatc
     1381 cgcaaccgca ttggcgagaa aaaatggctg ctgcgcgccg cgctgtacgg tattccgctg
     1441 ccgtggattg ctgtagaagc gggctggttc gtggctgaat atggccgcca accgtgggct
     1501 atcggtgaag tgctgccgac agctgtggcg aactcgtcac tgaccgcagg cgatctcatc
     1561 ttctcgatgg tgctgatttg cggcctgtat accctgttcc tggtggcaga attgttctta
     1621 atgttcaagt ttgcacgcct cggcccaagc agcctgaaaa ccggtcgcta tcactttgag
     1681 cagtcttcca cgactactca gccggcacgc taagacagga gtcgtcaaat gatcgattat
     1741 gaagtattgc gttttatctg gtggctgctg gttggcgttc tgctgattgg ttttgcagtc
     1801 actgacggtt tcgacatggg ggtgggcatg ctcacccgtt tcctcggtcg taacgacacc
     1861 gagcgtcgaa ttatgattaa ctcgatcgca ccacactggg acggtaacca ggtttggctg
     1921 atcaccgcag gcggcgcact gtttgctgcc tggccgatgg tctatgccgc tgcgttctcc
     1981 ggcttctatg tggcgatgat cctcgtgctg gcgtctttgt tcttccgtcc ggtcggtttt
     2041 gactaccgct ccaagattga agaaacccgc tggcgtaaca tgtgggactg gggcatcttc
     2101 attggtagct tcgttccgcc gctggtgatt ggtgtagcgt tcggtaacct gttgcagggc
     2161 gtaccattca acgttgatga atatctgcgt ctgtactaca ccggtaactt cttccagctg
     2221 cttaacccgt tcggcctgct ggcaggtgtg gtgagcgtag ggatgatcat tactcagggc
     2281 gcaacctatc tgcaaatgcg taccgtgggc gaactgcacc tgcgtacccg tgcaacggct
     2341 caggtggcgg cactggtgac gctggtctgc ttcgcactgg ctggcgtatg ggtgatgtac
     2401 ggtatcgatg gttatgtcgt gaaatcgaca atggaccatt acgcagcctc taacccgctg
     2461 aataaagaag tggttcgtga agctggcgca tggctggtta acttcaacaa cacgccaatt
     2521 ctgtgggcta ttccggcact gggtgtggtt ctgccgctgc tgaccatcct gactgcacgt
     2581 atggataaag ccgcgtgggc gtttgtgttc tcctccctga cgctggcctg catcatcctg
     2641 actgccggta tcgcaatgtt cccgtttgtg atgccgtcca gcaccatgat gaacgcaagt
     2701 ctgacaatgt gggatgcaac ttccagccag ctgacgctta acgtcatgac ctgggttgcg
     2761 gtggttctgg taccgatcat tctgctctac accgcctggt gttactggaa aatgttcggt
     2821 cgtatcacca aagaagatat tgaacgtaac acccactctc tgtactaagt aaggagctaa
     2881 aaatgtggta tttcgcatgg attctgggaa cgcttcttgc ctgttcgttt ggggtaatca
     2941 ccgcgctggc gcttgagcac gtcgaatcag gcaaagccgg tcaagaagac atctgatgag
     3001 taagattatc gcaaccttgt atgcggtaat ggacaagcgc cccttgcggg cgctttcctt
     3061 cgtgatggca cttctgttag caggatgtat gttttgggac ccatcacgtt tcgccgcgaa
     3121 gaccagtgaa ctggaaatct ggcatggttt actgctgatg tgggccgtct gtgctggtgt
     3181 gattcacggc gtgggctttc gtccgcagaa ggttctctgg caagggattt tttgcccatt
     3241 gcttgccgat attgttctca ttgtcgggct gattttcttc ttcttttaaa tcagaattct
     3301 ctttaaaaat ttatgggccg agtcaggccc ataaattttt acgctccctt aacttgccct
     3361 cattcccaaa cctcaatcgc gcgcgtatag tagcagcgtt taaaagctct aacttttgtt
     3421 gcattaccgg gatgtaaagt gaatacaacg ctgtttcgat ggccggttcg cgtctactat
     3481 gaagataccg atgccggtgg tgtggtgtac cacgccagtt acgtcgcttt ttatgaaaga
     3541 gcacgcacag agatgctgcg tcatcatcat ttcagtcaac aggcgctgat ggctgaacgc
     3601 gttgcctttg tggtacgtaa aatgacggtg gaatattacg cacctgcgcg gctcgacgat
     3661 atgctcgaaa tacagactga aataacatca atgcgtggca cctctttggt tttcacgcaa
     3721 cgtattgtca acgccgagaa tactttgttg aatgaagcag aggttctggt tgtttgcgtt
     3781 gacccactca aaatgaagcc tcgtgcgctt cccaagtcta ttgtcgcgga gtttaagcag
     3841 tgactgacat gaatatcctt gatttgttcc tgaaggctag ccttctggtt aaacttatca
     3901 tgttgatttt gattggtttt tcaatcgcat cctgggccat tattatccag cggacccgta
     3961 ttcttaacgc agcggcgcgc gaagccgaag cgtttgaaga taaattctgg tctggaatcg
     4021 aactctctcg cctctatcaa gagagccagg ggaaacggga taatctgagc ggttcggaac
     4081 aaatctttta cagcggcttc aaagagtttg tgcgcctgca tcgtgccaat agccatgcgc
     4141 cggaagccgt agtggaaggg gcgtcgcgtg cgatgcgtat ttccatgaat cgtgaacttg
     4201 aaaatctgga aacgcacatt ccgttcctcg gtacggttgg ctccatcagc ccgtatattg
     4261 gtctgtttgg tacggtctgg gggatcatgc acgcctttat cgccctcggg gcggtaaaac
     4321 aagcaacact gcaaatggtt gcgcccggta tcgcagaagc gttgattgcg acggcaattg
     4381 gtttgttcgc cgcaattccg gcggtaatgg cttataaccg cctaaaccag cgcgtaaaca
     4441 aactggaact gaattacgac aactttatgg aagagtttac cgcgattctg caccgccagg
     4501 cgtttaccgt tagcgagagc aacaaggggt aagccatggc cagagcgcgt ggacgaggtc
     4561 gtcgcgatct caagtccgaa atcaacattg taccgttgct ggacgtactg ctggtgctgt
     4621 tgctgatctt tatggcgaca gcgcccatca tcacccagag cgtggaggtc gatctgccag
     4681 acgctactga atcacaggcg gtgagcagta acgataatcc gccagtgatt gttgaagtgt
     4741 ctggtattgg tcagtacacc gtggtggttg agaaagatcg tctggagcgt ttaccaccag
     4801 agcaggtggt ggcggaagtg tccagccgtt tcaaggccaa cccgaaaacg gtctttctga
     4861 tcggtggcgc aaaagatgtg ccttacgatg aaataattaa agcactgaac ttgttacata
     4921 gtgcgggtgt gaaatcggtt ggtttaatga cgcagcctat ctaaacatct gcgtttccct
     4981 tgcttgaaag agagcgggta acaggcgaac agtttttgga aaccgagagt gtcaaaggca
     5041 accgaacaaa acgacaagct caagcgggcg ataattattt cagcagtgct gcatgtcatc
     5101 ttatttgcgg cgctgatctg gagttcgttc gatgagaata tagaagcttc agccggaggc
     5161 ggcggtggtt cgtccatcga cgctgtcatg gttgattcag gtgcggtagt tgagcagtac
     5221 aaacgtatgc aaagccagga atcaagcgcg aagcgttctg atgagcagcg caagatgaag
     5281 gagcagcagg ctgctgaaga actgcgtgag aaacaagcgg ctgaacagga acgcctgaag
     5341 caacttgaga aagagcggtt agcggctcag gagcagaaaa atcaggctga agaagccgca
     5401 aaacaggccg agttaaagca gaagcaagcg gaagaggcgg cagcgaaagc ggcggcagat
     5461 gctaaagcga aggccgaagc ggatgctaaa gctgcggaag aagcagcgaa gaaagcggcg
     5521 gcagacgcga agaaaaaagc agaagcagaa gccgccaaag ccgcagtcga agcgcagaaa
     5581 aaagccgagg ctgctgctgc ggcgctgaag aagaaagctg aagcggcaga agcagctgca
     5641 gctgaagcaa gaaagaaagc ggcaaccgaa gctgctgaaa aagccaaggc agaagctgag
     5701 aagaaagcgg ctgctgaaaa ggctgcagct gataagaaag cggcagcaga gaaagcggca
     5761 gccgacaaaa aagcagcaga aaaagcggct gctgaaaagg cagcagctga taagaaagca
     5821 gcggcagaaa aagccgccgc agacaaaaaa gcggcagcgg caaaagcagc agctgaaaaa
     5881 gccgctgcag caaaagctgc cgcagaggca gatgatattt tcggtgagct aagctctggt
     5941 aagaatgcac cgaaaacggg gggaggggcg aaagggaaca atgcttcgcc tgccgggagt
     6001 ggtaatacta aaaacaatgg cgcatcaggg gccgatatca ataactatgc cgggcagatt
     6061 aaatctgcta tcgaaagtaa gttctatgac gcatcgtcct atgcaggcaa aacctgtacg
     6121 ctgcgcataa aactggcacc cgatggtatg ttactggata tcaaacctga aggtggcgat
     6181 cccgcacttt gtcaagctgc gttggcagca gctaaacttg cgaagatccc gaaaccacca
     6241 agccaggcag tatatgaagt gttcaaaaac gcgccattgg acttcaaacc gtaatcgcga
     6301 tgttgactgt tccgacggtc aacatcaggc accggttgcc acggggttct ggtagttttg
     6361 tgtattttag tttgttaaca ttctgctaaa ttatcgtggg ccatcggtcc agataaggga
     6421 gatatgatga agcaggcatt acgagtagca tttggttttc tcatactgtg ggcatcagtt
     6481 ctgcatgctg aagtccgcat tgtgatcgac agcggtgtag attccggtcg tcctattggt
     6541 gttgttcctt tccagtgggc ggggcctggt gcggcacctg aagatattgg cggcatcgtt
     6601 gctgctgact tgcgtaacag cggtaaattt aatccgttag atcgtgctcg tctgccacag
     6661 cagccgggta gtgcgcagga agtacaacca gctgcatggt ccgcgctggg cattgacgct
     6721 gtggttgtcg gtcaggtcac tccgaatccg gatggttctt acaatgtcgc ttatcaactt
     6781 gttgacactg gcggcgcacc gggtactgta cttgctcaga actcgtacaa agtgaacaag
     6841 cagtggctgc gttatgctgg tcataccgcc agtgacgaag tatttgaaaa actgaccggt
     6901 attaaaggtg cgttccgtac ccgtattgcc tacgttgttc agactaacgg cggtcagttc
     6961 ccgtatgaac tgcgcgtatc tgactatgac ggttacaacc agtttgtcgt tcaccgttca
     7021 ccgcaaccgc tgatgtctcc ggcgtggtca ccagacggtt ctaaactggc ttatgtgacc
     7081 ttcgaaagcg gtcgttccgc gctggttatt cagacgctgg caaatggcgc tgtacgtcag
     7141 gtggcttcat tcccgcgtca caacggtgca cctgcattct cgccagacgg cagcaaactg
     7201 gcattcgcct tgtcgaaaac cggtagcctg aacctgtacg taatggattt ggcttctggt
     7261 cagatccgcc aggtaactga tggtcgcagt aacaataccg aaccgacctg gttcccggac
     7321 agccagaacc tggcatttac ttctgaccag gccggtcgtc cgcaggttta taaagtgaat
     7381 atcaacggcg gtgcgccaca acgtattacc tgggaaggtt cgcagaacca ggatgcggat
     7441 gtcagcagcg acggtaaatt tatggtaatg gtcagctcca acggtgggca gcagcacatt
     7501 gccaaacaag atctggcaac gggaggcgta caagttctgt cgtccacgtt cctggatgaa
     7561 acgccaagtc tggcacctaa cggcactatg gtaatctaca gctcttctca ggggatggga
     7621 tccgtgctga atttggtttc tacagatggg cgtttcaaag cgcgtcttcc ggcaactgat
     7681 ggacaggtca aattccctgc ctggtcgccg tatctgtgat aataattaat tgaatagtaa
     7741 aggaatcatt gaaatgcaac tgaacaaagt gctgaaaggg ctgatgattg ctctgcctgt
     7801 tatggcaatt gcggcatgtt cttccaacaa gaacgccagc aatgacggca gcgaaggcat
     7861 gctgggtgcc ggcactggta tggatgcgaa cggcggcaac ggcaacatgt cttccgaaga
     7921 gcaggctcgt ctgcaaatgc aacagctgca gcagaacaac atcgtttact tcgatctgga
     7981 caagtacgat atccgttctg acttcgctca aatgctggat gcacacgcaa acttcctgcg
     8041 tagcaacccg tcttacaaag tcaccgtaga aggtcacgcg gacgaacgtg gtactccgga
     8101 atacaacatc tccctgggtg aacgtcgtgc gaacgctgtt aagatgtatc tgcagggtaa
     8161 aggcgtttct gctgaccaga tctccatcgt ttcttacggt aaagaaaaac ctgcagtact
     8221 gggtcatgac gaagcggcgt atgccaaaaa ccgtcgtgcg gtactggttt actaagagaa
     8281 ttgcatgagc agtaacttca gacatcaact attgagtctg tcgttactgg ttggtatagc
     8341 ggccccttgg gccgcttttg ctcaggcacc aatcagtagt gtcggctcag gctcggtcga
     8401 agaccgcgtc actcaacttg agcgtatttc taatgctcac agccagcttt taacccaact
     8461 ccagcaacaa ctctctgata atcaatccga tattgattcc ctgcgtggtc agattcagga
     8521 aaatcagtat caactgaatc aggtcgtgga gcggcaaaag cagatcctgt tgcagatcga
     8581 cagcctcagc agcggtggtg cagcggcgca atcaaccagc ggcgatcaaa gcggtgtggc
     8641 ggcatcaacg acgccgacag ctgatgctgg tactgcgaat gctggcgcgc cggtgaaaag
     8701 cggtgatgca aacacggatt acaatgcagc tattgcgctg gtgcaggata aatcccgcca
     8761 ggatgacgca atggtggcat ttcagaattt catcaaaaat taccctgatt caacttacct
     8821 gccaaacgcc aattattggc tgggtcagtt aaactacaac aagggtaaaa aagatgatgc
     8881 ggcgtattat tttgcttcgg tagtgaaaaa ctatccgaag tcaccgaagg ctgcagatgc
     8941 gatgtttaaa gtcggcgtca tcatgcagga caaaggtgac accgcaaaag cgaaagccgt
     9001 ataccagcag gttatcagta aataccctgg tactgatggc gctaaacagg cgcaaaaacg
     9061 tctgaacgcg atgtaatgca taacacacga ccagaagtcg cattatttct ggtcgtgtcg
     9121 tgcgaatcat aagcagttga gtgatctaca tcgaaatttt tgttgcgctc aagtctgaaa
     9181 tcagtaatat atgccgccgt tgccacggga tatcaaacaa accgaaagca acgaaaaagt
     9241 gggtcgttag ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat
     9301 cctgcacgac ccaccactaa catagttagt tgtagtatcc agcgtagtat cgggtgatta
     9361 gctcagctgg gagagcacct cccttacaag gagggggtcg gcggttcgat cccgtcatca
     9421 cccaccaccg ggtcgttagc tcagttggta gagcagttga cttttaatca attggtcgca
     9481 ggttcgaatc ctgcacgacc caccactgat tacggtgggt tcggtggaag tagtttgtag
     9541 tatccagcgc agtatcgggt gattagctca gctgggagag cacctccctt acaaggaggg
     9601 ggtcggcggt tcgatcccgt catcacccac cactcgggtc gttagctcag ttggtagagc
     9661 agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aattttaaac
     9721 attggttccg agtagaaatc tttcaggtaa cacccgtatg ggtcgttagc tcagttggta
     9781 gagcagttga cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccatcctg
     9841 aatgattaaa gcagcataat cccgcaaggg gtcgttagct cagttggtag agcagttgac
     9901 ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatgcaa aaaagcgccc
     9961 taaaggcgct tttttgctat tcaggcatct ctcaatttca attcttgacc ctgatgacat
    10021 actccgcgct tgctgcgtaa acaactctac gtcggaggaa taagacgcaa agtgtggcat
    10081 tcggcatctc atacgtaatt cgagtgactt ttccccacca ttcgactatc ttgtttagca
    10141 tataaaacaa attacgccga taatagcgaa tatcacgctt aagtcggttt taacgttaag
    10201 cctgtaaaac gagatggtaa gatgagcgtg atgtttgatc cagacacggc gatttatcct
    10261 ttccccccga agccgacgcc gttaagcatt gatgaaaaag cgtattaccg cgagaagata
    10321 aaacgtctac taaaagaacg taatgcggtg atggttgccc actactatac cgatcccgaa
    10381 attcaacaac tggcagaaga aaccggtggc tgtatttctg attctctgga aatggcgcgc
    10441 ttcggtgcaa agcatcccgc ttctacgttg ttagttgctg gggtgagatt tatgggagaa
    10501 accgccaaaa ttctcagtcc ggaaaaaaca attctgatgc cgacacttca ggctgaatgt
    10561 tcactggatc tcggctgccc tgttgaagaa tttaacgcat tttgcgatgc ccatcccgat
    10621 cgtactgtcg tcgtctacgc caacacttct gctgcggtaa aagcgcgcgc agattgggtg
    10681 gtaacttcaa gcattgccgt cgaacttatt gatcatctgg atagtttggg tgaaaaaatc
    10741 atctgggcac ctgacaaaca tctggggtgt tacgtgcaaa aacagacggg cgcagacatt
    10801 ttatgctggc agggtgcctg cattgtgcat gatgagttta agactcaggc gttaacccgc
    10861 ttgcaagaag aatacccgga tgctgccata ctggtgcatc cagaatcacc gcaagctatt
    10921 gtagagatgg cggatgcggt cggttccacc agtcaactga tcgctgctgc gaaaacattg
    10981 ccacatcagc ggcttatcgt ggcaaccgat cggggtattt tctacaaaat gcagcaggcg
    11041 gtgccagata aagagttact ggaagcacca accgcgggtg agggcgcaac ctgtcgcagc
    11101 tgcgcgcatt gcccgtggat ggccatgaat ggccttcagg ccatcgcaga ggcattagaa
    11161 ctggaaggaa gcaatcacga ggtttatgtt gatgaaaggc tgctagagag ggcgctggtg
    11221 ccgctcaatc gtatgctgga ttttgcggct acactacgtg gataattgat aataaggcgt
    11281 cacattacgc tttgggggaa cgatggattt ttttagtgtg cagaatatct tggtacatat
    11341 accaataggg gcaggcggtt atgatctctc atggatcgaa gcggtaggca cgatcgccgg
    11401 gttgctgtgt attggccttg ccagtctgga gaagatcagt aactacttct ttggcctgat
    11461 caacgtcacc ttgtttggca ttattttctt tcagattcag ctgtatgcca gcctgctatt
    11521 acaggtgttt ttctttgccg cgaatattta cggctggtat gcgtggtcgc gacaaaccag
    11581 tcagaacgag gcggagttga aaattcgctg gttgccattg ccgaaggcgc taagctggtt
    11641 ggcggtttgc gttgtttcga ttggtctgat gacggtattt atcaatccgg tgtttgcatt
    11701 tttgacccgc gtggcagtca tgatcatgca agcattagga ttacaggttg cgatgcctga
    11761 actacagccg gacgctttcc cgttctggga ttcatgcatg atggtgttat ctatcgtggc
    11821 aatgattctg atgacgcgta agtatgtgga aaactggctg ttgtgggtga ttattaacgt
    11881 gattagcgtc gttatttttg cacttcaggg cgtttacgcc atgtctctgg agtacatcat
    11941 cctgaccttt attgccctca acggcagccg gatgtggatc aacagcgcac gcgaaagagg
    12001 ttcacgcgcg ctgtcccatt aatggtgatg atgtgaatgg ccagacaccc cttcattcag
    12061 gtggcagtct ggcccatgac aaggttgata ttccatctga atagtggcgt gctcaatctg
    12121 atagtgatcc atcaggtagt gttggatctg atctaacaag gcatcgtgat cgtgtggcgg
    12181 gatcacctgc acatgcagcg tcatcaccgg cttctcgcct accatccata catgcacatg
    12241 gtgcacattg cggacttccg ggatttcccg gcacaagcga cgcttcagtt cagcgatatc
    12301 cagcgatacc ggtgcacctt caagtaattc attcacacta tctttcaaca gtcgccacgc
    12361 gctacgcaga accagaagcg acaccagtat tgagagaatg gggtcagcag gtgtccagcc
    12421 ggtccagata ataatcaacg cggcaataat agccccaacc gaacccagca ggtccccgag
    12481 cacatgcagt gccgctgctc gcacgttgag gtttttctct tcgctaccgt gatgaagtaa
    12541 ccagaaagaa agtatatttg ccaacagccc agccacggca attgccatca tcatgccgcc
    12601 ctcgaccgga cgcggtgtgc ggaagcgttc tatcgcctcc cagacaatca aaatggtaat
    12661 caccaccagg gcgatagcat tcacaaatgc tgcgagcgtg gtcaatctta gccaaccgaa
    12721 agtgtggcga atggtgggag gtcgacgtga aaattgcacg gcgagtaggg cgaaaagcag
    12781 ggcggcagta tcggtcaaca tatgacccgc atcggccagc aatgccagag aaccggaaag
    12841 aaaaccgcca atgacttcta cgagcataaa cccggcagtc acgccgaaag catacaacaa
    12901 gcgacgagca ttattatctt ctggcaggtg tgaggaagcg tgtgagtgtg agtgcgccat
    12961 gacgagtcat cccttaatta ttctcactta actttatgac atcatactgc ttgtagaagg
    13021 gaaaaattaa aagggagaga ctccgctctc ccattattgg ctattttgca gggttactgc
    13081 gtggtaccgt cggttttggt atcgacatca ttattgatgc catcaccagt ttgtaccttt
    13141 ttattgatat ccgggcagcg accatcttta cacatggtgt tcttgtgctc ttcatctttg
    13201 gtcatgccgt cattgttcat tgaagaacca tccggatgca gcattgtgcc gtcggaaccg
    13261 gtatttaccc cgttattgtc gacgttattt ggcgcgacat tttcacgggc gtcaggggct
    13321 acctggcccg catcagctgc ggcgtttgcc tggccgttat tgctttgcgc tccgctatcg
    13381 gcagccagtg cggcaccgct ggcaaggctt agagtggcag tcaaaaatag tgtggccagt
    13441 tttgtcattt tcataggatg ctcctgttat ggtcgttatg tcggataacc tcttccaaca
    13501 gtgcatttgc aggtgaatat caggcattgg tttaagattt cagccagctt atgaaacgca
    13561 gcagagaatc ttgaaataat taacaaacaa aggagttaca gttagaaatt gtaggagaga
    13621 tctcgttttt cgcgacaatc tggcgttttt cttgctaatt ccaggattaa tccgttcata
    13681 gtgtaaaacc ccgtttacac attctgacgg aagatataga ttggaagtat tgcattcact
    13741 aagataagta tggcaacact ggaacagaca tgaattatca gaacgacgat ttacgcatca
    13801 aagaaatcaa agagttactt cctcctgtcg cattgctgga aaaattcccc gctactgaaa
    13861 atgccgcgaa tacggtcgcc catgcccgaa aagcgatcca taagatcctg aaaggtaatg
    13921 atgatcgcct gttggtggtg attggcccat gctcaattca tgatcctgtc gcggctaaag
    13981 agtatgccac tcgcttgctg gcgctgcgtg aagagctgaa agatgagctg gaaatcgtaa
    14041 tgcgcgtcta ttttgaaaag ccgcgtacca cggtgggctg gaaagggctg attaacgatc
    14101 cgcatatgga taacagcttc cagatcaacg acggtctgcg tattgcccgt aaattgctgc
    14161 ttgatattaa cgacagcggt ctgccagcgg cgggtgagtt tctcgatatg atcaccccac
    14221 aatatctcgc tgacctgatg agctggggcg caattggtgc acgtaccacc gaatcgcagg
    14281 tgcaccgcga actggcgtct ggtctttctt gtccggtagg tttcaaaaat ggcactgatg
    14341 gtacgattaa agtggctatc gatgccatta atgccgccgg tgcgccgcac tgcttccttt
    14401 cagtgaccaa atggggtcat tcggcgattg tgaataccag cggtaacggc gattgccata
    14461 tcattctgcg cggtggtaaa gagcctaact acagcgcgaa gcacgttgct gaagtgaaag
    14521 aagggctgaa caaagcaggc ctgccagcac aggtgatgat cgatttcagc catgctaact
    14581 cgtcgaaaca attcaaaaag cagatggatg tttgtgctga cgtttgccag cagattgccg
    14641 gtggcgaaaa ggccattatt ggcgtgatgg tggaaagcca tctggtggaa ggcaatcaga
    14701 gcctcgagag cggggaaccg ctggcctacg gtaagagcat caccgatgcc tgcattggct
    14761 gggatgatac cgatgctctg ttacgtcaac tggcgaatgc agtaaacgcg cgtcgcgggt
    14821 aaagtttaat tgtcggatgc gccgtcagag tggcgtatcc gattaatcac cggaggcctg
    14881 ataaaacgcg cagcgtcgca tcaggcaatg tgctccattg gtagcaacga aaaataaaaa
    14941 agccgactca ttttgcagtc ggctttctca tttttaagcg aatgacgttt acttcgcttt
    15001 accctggtta gcaaccgccg ctgctttcgc cgcgatctcg tcagcattac ccagataata
    15061 gcgtttcagc ggtttgaagt tctcgtcaaa ctcataaacc agcggcacgc cggtcgggat
    15121 attcagctca agaatctctt cttcgctcat gttatcgaga tatttcacca gcgcacgtaa
    15181 cgagttaccg tgtgcggcga tgatgacgcg ctcaccgctc ttcatacgcg gcagaatggt
    15241 ttcattccag taagggatca cgcggtcaat ggtcagcgcc aggctttccg tcagcggcag
    15301 ttctttctcg ctcagtttcg cgtaacgcgg atcgtgaccc ggataacgct catcatcttt
    15361 agtcagttcc ggcggagtca ccgcaaaacc acgacgccac tgtttcacct gctcgtcgcc
    15421 atacttttca gcggtttccg ctttgttcag accctgcaac gcaccgtagt gacgttcgtt
    15481 cagtttccag gatttctcaa cgggcagcca tgcctgatcc agttcgtcca gcacattcca
    15541 cagggtatga atagcgcgtt tcagcacaga ggtgtaagca aagtcaaagc tgtaaccttc
    15601 ctctttcagc agcttacctg ctgcttttgc ttcgcttacg cctttctcag acagatccac
    15661 gtcgtaccaa ccggtgaaac ggttttcttt gttccactga ctttcgccat gacgaaccag
    15721 aaccagctta gttacagcca tatacttact cctcaaatca tcttttaatg ataataattc
    15781 tcattatatt gccgtgacga agcaacagca atgcttacgc ataaccatag cgaaaatagt
    15841 gtcgcagtgt aaggttgttg tgaatattga gttgcaaata tgtcggtgtt tgctggtgat
    15901 ttgaacaata tgaagataaa gccctcatga cgagggctta acattactga gcaataaact
    15961 gatattccgt caggctggaa tactcttcgc caggacgcag gaagcagtcc ggttgcggcc
    16021 attcagggtg gttcgggctg tccggcagaa attcgctttc cagagccagc ccttgccagt
    16081 cggcgtaagg ttcggttccc cgcgacggtg tgccgccgag gaagttgccg gagtagaatt
    16141 gcagagccgg agcggtggtg tagaccttca gctgcaattt ttcatctgct gaccagacat
    16201 gcgccgccac tttcttgcca tcgcctttgg cctgtaacaa gaacgcgtga tcgtaacctt
    16261 tcactttgcg ctgatcgtcg tcggcaagaa actcactggc gatgattttg gcgttgcgga
    16321 aatcaaaaga ggttccggcg acagatttca ggccgtcgtg cggaatgccg ccttcatcaa
    16381 ccggcagata ttcttccgcc agaatctgca acttgtgatt gcgcacgtca gattgctcgc
    16441 cgtcaagatt gaaatagacg tgattagtca tattcaccgg gcaaggttta tcaactgtgg
    16501 cgcgataagt aatggagata cggttatcgt cggtcagacg atattgcacc gtcgcgccga
    16561 gattacccgg gaaaccctga tcgccatcat ctgaactcag ggcaaacagc acctgacgat
    16621 cgttctggtt cacaatctgc cagcgacgtt tgtcgaaccc ttccgggccg ccatgcaatt
    16681 ggttgacgcc ctgacttggt gaaagcgtca cggtttcacc gtcaaaggta taacggctat
    16741 tggcgatgcg gttggcataa cgaccaatag aggcccccag aaatgcggcc tgatcctgat
    16801 agcattccgg gctggcgcag ccaagcagcg cctcgcggac gctgccatcg gaaagcggaa
    16861 tacgggcgga aagtaaagtc gcaccccagt ccatcagcgt gactaccatc cctgcgttgt
    16921 tacgcaaagt taacagtcgg tacggctgac catcgggtgc cagtgcggga gtttcgttca
    16981 gcactgtcct gctccttgtg atggtttaca aacgtaaaaa gtctctttaa tacctgtttt
    17041 tgcttcatat tgttcagcga cagcttgctg tacggcaggc accagctctt ccgggaacaa
    17101 cgcgacgata cagccaccaa atccgccgcc ggtcatgcgt acgccacctt tgtcgccaat
    17161 cacagctttg acgatttcta ccagagtgtc aatttgcggt acggtgattt cgaaatcatc
    17221 gcgcatagag gcatgagact ccgccatcaa ctcgcccata cgtttcaggt cgccttgctc
    17281 cagtgcgctg gcagcttcaa cggtgcgggc gttttcagtc aggatatgac gcacgcgttt
    17341 cgccacgatt gggtccagtt catgcgcaac agcgttgaac tcttcaatgg tgacatcgcg
    17401 cagggctggc tgctggaaga aacgcgcacc ggtttcgcac tgttcacgac gggtgttgta
    17461 ttcgctgcca accagggtac gtttgaagtt actgttgatg atgacgacag ccacaccttt
    17521 gggcatggaa actgctttgg tccccagtga gcggcagtca atcagcaagg catgatcttt
    17581 cttgccgagt gcggaaatca gttgatccat gattccacag ttacagccta caaactggtt
    17641 ttctgcttcc tgaccattaa gcgcgatttg tgcgccgtcc agcggcagat gataaagctg
    17701 ctgcaatacg gttccgaccg cgacttccag tgaagcggaa gaacttaacc ctgcaccctg
    17761 cggcacattg ccgctgatca ccatgtccac accgccgaag ctgttgttac gcagttgcag
    17821 atgtttcacc acgccacgaa cgtagttagc ccattgatag ttttcatgcg cgacaatggg
    17881 cgcatcgagg gaaaactcgt cgagctgatt ttcataatcg gctgccatta cgcgaacttt
    17941 acggtcatcg cgtggcgcac agctgatcac ggtttgataa tcaatcgcgc agggcagaac
    18001 gaaaccgtcg ttgtagtcgg tgtgttcacc aatcaaattc acgcggccag gcgcctgaat
    18061 ggtgtgagtg gcagggtagc caaatgcgtt ggcaaacaga gattgtgttt tttctttcag
    18121 actcatttct tacactccgg attcgcgaaa atggatatcg ctgactgcgc gcaaacgctc
    18181 tgctgcctgt tctgcggtca ggtctcgctg ggtttctgcc agcatttcat aaccaaccat
    18241 aaatttacgt acggtggcgg agcgcaacag aggcggataa aagtgcgcgt gcagctgcca
    18301 gtgttgattc tcttcgccat taaatggcgc gccgtgccag cccatagagt aggggaagga
    18361 gcactggaag aggttgtcat aacgactggt tagctttttc aacgccagtg ccaaatcgct
    18421 gcgctgggcg tcggtcaaat cggtgatccg caaaacgtgg gctttgggca gcagtagcgt
    18481 ttcgaacggc caggcagccc agtaaggtac aacggctaac cagtgttcgg tttcgacaac
    18541 ggtacggcta ccgtctgcca gctcgcgctg aacatagtcc accagcattg gcgatttctg
    18601 tgcggcgaaa tattcttttt gcagacggtc ttcgcgctca gcttcgttag gcaggaagct
    18661 atttgcccaa atctgtccgt gcggatgcgg gttagagcag cccatcgccg cgcctttgtt
    18721 ttcaaagacc tgcacccatg ggtacgtttc ccccagctct gcggtttgct cctgccaggt
    18781 tttgacgatt tccgtcaatg ccgcaacgct cagttctggc aacgttttac tgtgatccgg
    18841 tgaaaagcag atcacccggc tggtaccgcg cgcgctctgg caacgcatta gcggatcgtt
    18901 gctttctggc gcatctggcg tgtcagacat caaggccgca aagtcattag tgaaaacgta
    18961 agtcccggtg taatcggggt ttttatcgcc tgtcacccgc acattacctg cgcagaggaa
    19021 gcaatctgga tcgtgcgcag gtaacacctg tttggctggc gtttcctgcg ccccctgcca
    19081 ggggcgctta gcgcggtgcg gtgaaaccag aatccattgc ccggtgagcg ggttgtagcg
    19141 gcgatgtgga tgatcaacgg gattaaattg cgtcatggtc gttccttaat cgggatatcc
    19201 ctgtggatgg cgtgactgcc agtgccaggt gtcctgcgcc atttcatcga gtgtgcgcgt
    19261 tacgcgccag ttcagttcac ggttggcttt gctggcgtcc gcccagtagg ccggaaggtc
    19321 gccctcgcga cgcggtgcaa aatgataatt aaccggtttg ccgcaggctt tgctgaaggc
    19381 attaaccacg tccagcacgc tgctgcccac gccagcgccg aggttgtaga tgtgtacgcc
    19441 tggcttgttc gccagttttt ccatcgccac gacgtgaccg tccgccagat ccattacgtg
    19501 gatgtaatcg cgcacgccag taccgtcttc ggtcggataa tcgttaccaa aaatcgccag
    19561 cgagtcgcga cggcctacag caacttgggc gatgtatggc atcaggttat tcggaatgcc
    19621 ttgcggatct tcgcccatat cgcccgacgg atgcgcgcca actgggttga agtagcgcag
    19681 cagggcaatg ctccagtccg gctgggcttt ttgcagatcg gtgaggatct gttccaccat
    19741 cagcttgctt ttgccgtaag ggctttgcgg tgtgccggtc gggaagcttt caacatatgg
    19801 aattttgggc tgatcgccat aaacggtggc ggaggagcta aagataaagt ttttgacgtt
    19861 agcggcgcgc atggcgctaa tcaggcgcag agtaccgttg acattgttgt cgtaatattc
    19921 aagcggtttt tgtaccgatt cgccaacggc tttcagcccg gcgaagtgga tcacggtgtc
    19981 gatagcgtga tcgtgcagga tctcggtcat caacgcttcg ttacggatat cgccttcaac
    20041 aaacgttgga tgtttgccgc ctaaacgctc gataacaggc agtacgctgc gcttactgtt
    20101 acagaggtta tcaagaatga tgacatcatg accgttttgc agtaattgca cacaggtatg
    20161 acttccaatg taaccgctac caccggtaac cagaactctc ataattcgct ccattaggct
    20221 tatggtatga aataaccata gcataacaaa gatgcgaaaa gtgtgacatg gaataaatta
    20281 gtggaatcgt ttacacagga atttagccgt tttttatgcg cgattaagtg attataaaac
    20341 agcggcttta tgaatgattg cgctttttat ctgaaaaaaa agacgcggtt tcatgcctgg
    20401 atgcgtcaaa ccgttggccg gataacacgt ttacgccgcc tccggcacgg tcagcactac
    20461 cgactcagtt tatttttgtc agcgcatagc gataaaagtc accgtccggc acgaactcaa
    20521 gacgatgggt aatacaggca ggcgcatctt cagcgtggtg cgaaacaaac aacaattgcg
    20581 ttttaccttc gctaatcagc acatcaacaa aacggcggat aagctggcga ttgagcggat
    20641 caagcccctg tagtggttca tcgagaataa gcaacgtcgg atgtttcact agcgcacgga
    20701 cgatcagcgc cagacgctgc tgtccccagg aaagactatg gaaaggagcg tcagccgtgc
    20761 gtttatcaat gccgagaata tccagccact gctgcaccag tttttgctgg cgatccgaaa
    20821 cggcctgata aatgccaatc gaatcaaaat agccagaaag aatcacatta cgcacagtag
    20881 tgctgacccg gtaatccaga tgcaaactac tgctgacgta accgatatgc tttttgatat
    20941 cccagatggt ttcgccgctg ccgcgacgtc gtccgaaaag cgtcaaatcg ttgctgtaac
    21001 cttgcggatg atcgccagta atcaggctta ataacgtcga ttttcccgcg ccatttggcc
    21061 cgacaatttg ccagtgttcg cctggattca cctgccagct aaggttatta aggatggggc
    21121 gttcgttata agaaaccacg ccattgttca gcacaatgcg cggttcgtgg gcgggtaagg
    21181 cgtgacgtac tgaaggttca tccggctccg gcatttgcac acctacaagc agttcgctat
    21241 gcgccagttg cgcaacgagt gcttgttgga gcagttcttc tttagcgcca gtttccgcta
    21301 acgtgcaatc cgccagcacg ccagcaaact ggacaaattc cgggatctca tcgaagcgat
    21361 tgagcaccag taccagagta ataccggact gatgtaacga ggcgagtagc tcagccagct
    21421 gctgacgtga ggcaacatcc aggccatcaa acggttcatc aaggatcaac aagtcaggct
    21481 ccgacatcag cgcctgacac agcagggttt ttcgcgtctc gccagtggaa aggtatttaa
    21541 agcgtcggtc gaggagggcg gtaataccga acagctgcgc cagttgcatg caacgcggag
    21601 tatcctttac ttcatcctga atgatctcag ccgtggtacg cccggtgtca tcttcgccag
    21661 gactaagcat atcggtgtta ttccgctgcc attcgtcgct gacgagcttt tgcaattgct
    21721 cgaaggagag acgagtgatg tgggaaaact ggctttgccg ttcacctttc aaaagcggaa
    21781 gttcccccga cagcgcgcgg gccagcgccg atttcccgct tccattcgaa ccgacaaacg
    21841 cccaactatc acccgcgttt aacgttagcc gaggcaattg cagcgttttt gtgtcgccaa
    21901 gacgaaacgt gccttgcaaa atttgcaacg atgacatttt atatcccatt ttgtgcagcg
    21961 attactgaca gggatacgtg tttcataaca aattgtcaac acgcttagca cagcgtggcg
    22021 ataatcacgc tgtcggcatt aaagtaggtc gtgacattct gcccttgctg aagagaagtc
    22081 gcttcattta ccggcactgt ggcgcacagt gtttgcccgt cgggtagcgc cattaatact
    22141 tcgcactgct ctgcgccgcg ctcaatatga ctaataatac ctggtaattg gttgtcagcg
    22201 ttttgcgcga ccgcatcgtc ctgagtaata cctacccacg gcgcttttag caatatcaac
    22261 acttctttgc cttcatccag ccccagacgc gcgccgcttt gtgcggtaac tgcgactttc
    22321 aggcgtgttt ttccatcagc cagtaagaca tcaacatgct gttgaacgtc atcatgatcg
    22381 cgggcggtga tggtgccaaa ccactggtta cgggcgctgg tttgcagtga aaaacgtgag
    22441 atcgcggcca gcaggctgtt cagcggcagg gcgtcatcgt cacttaacac atcaaaggct
    22501 ttttgctgga tttgcgccag taagtcatag agctgaatca gtcgctgacc atagcgggtc
    22561 agtactgcgc cgcctccacc tttaccgcct gttgcgcgct caaccagaat atgctcactt
    22621 aactgattca tctcgttaat ggcatcccag gcgcttttat agctgatacc ggcatctttc
    22681 gctccctggc taatggaacc ggaaagcgca atgtgtttta gtagcgaaat gcggcgcggg
    22741 tcggcgaata atttttgttg gagcttaagg gtgagaagga tttcggcctg cataacaatg
    22801 tcctggcaaa agtcttattg tgacggaaaa cgaacgccac gcaaagctga ccgcacaaaa
    22861 ggggagtgct tttctgtgct tagcggttag aatagtctca tgactatatc tggagttgac
    22921 catgttagag ttattaaaaa gtctggtatt cgccgtaatc atggtacctg tcgtgatggc
    22981 catcatcctg ggtctgattt acggtcttgg tgaagttttc aacatctttt ctggtgttgg
    23041 taaaaaagac cagcccggac aaaatcattg attccctgaa tgcccgctta gtcgggcatt
    23101 ttctttttct caacttcctg ctttttctgc cgatattttt tctcatctac ctcacaaagg
    23161 ttagcaataa ctgctgggaa aattccgaac ttgccgttat attgacgcct acataacgtt
    23221 acattaaggg gttaccaatg gctcgtaaat ggttgaactt gtttaccggg gcggcactct
    23281 ctttcgctgt tgctggcaat gcactggcag atgaagggaa aatcacggtg ttcgccgccg
    23341 catcactgac taacgcaatg caggacattg ctacgcagta taaaaaagag aaaggcgtgg
    23401 atgtggtttc ttctttcgct tcgtcatcta ctttggcccg tcagattgaa gcgggtgcgc
    23461 ctgcggatct gtttatttct gccgatcaga aatggatgga ttatgcggtt gataaaaaag
    23521 cgatcgatac ggcttcgcgc cagacactgc tcggcaatag cctggtcgtt gtagcaccga
    23581 aagccagcga gcagaaagat ttcaccatcg acagcaaaac caactggact tcgctgctga
    23641 atggcggtcg cctcgcggtt ggtgatccgg aacatgtccc tgctggcatt tatgcgaaag
    23701 aagcactgca aaaactgggc gcatgggata cgctctcccc gaaactggct ccggcggaag
    23761 atgttcgtgg cgcgctggcg ttggtcgaac gcaatgaagc tccgctgggt attgtttacg
    23821 gttctgacgc agttgccagc aaaggggtaa aagtggttgc caccttcccg gaagattcac
    23881 ataaaaaagt ggaatatccg gttgctgttg tggaagggca taacaatgcg acagtgaaag
    23941 cattttatga ttatctgaag gggccgcagg ctgcggaaat ctttaaacgt tacggattta
    24001 caaccaagta atgatactga ccgatccaga atggcaggca gttttattaa gcctgaaagt
    24061 ttcttccctg gctgtgctgt ttagcctgcc gtttgggatc ttttttgcct ggttactggt
    24121 acgttgcacg tttccgggca aagctctgct cgacagcgta ctgcatctac cgctggtgtt
    24181 accgcccgtg gtcgtcggtt acctattatt agtttcgatg ggacggcgcg gatttatcgg
    24241 tgaacgtctg tatgactggt ttggtctttc tttcgcgttt agctggcgtg gtgcggttct
    24301 cgctgccgct gtcatgtctt ttccgctgat ggtgcgggcg attcgtctgg cgctggaagg
    24361 tgttgatatc aaactggagc aggccgcaag aacactgggg gccgggcgct ggcgcgtttt
    24421 ctttactatc acgttaccgc tgaccttacc gggaattatt gttggtacgg tgctggcatt
    24481 cgctcgttcg ctcggtgaat ttggcgcaac catcaccttt gtttctaaca ttcctggtga
    24541 aacgcgaacc attccttctg ccatgtatac cctgatccag acccctggcg gggaaagtgg
    24601 cgcggcgaga ctgtgtatta tttctattgc gctggcgatg atctccctgt tgatttcaga
    24661 atggctggcc agaatcagcc gtgaacgggc ggggcgctaa tcatgctgga actgaatttt
    24721 tcccagacgt tgggcaacca ttgcctgacg attaatgaaa cgctgcccgc caatggcatc
    24781 accgctattt ttggtgtctc tggtgccgga aaaacctcgc tgattaacgc catcagtggg
    24841 ctgacacgtc cgcaaaaagg gcggattgtc ctcaatggtc gggtactaaa tgatgccgaa
    24901 aaaggtatct gcctgtcgcc ggaaaagcgt cgcgttggct atgtttttca ggatgcgcgg
    24961 ctgttcccgc attacaaagt gcgtggcaat ctgcgctacg gcgtggcgaa aagcatggtc
    25021 aatcagttcg ataagctggt ggcgctttta ggcattgaac cgttgcttga tcgtttacca
    25081 ggcagcctgt ccggaggcga aaaacagcgc gtggcgattg gtcgggcttt gctgacagca
    25141 ccggaattgc tgttgctgga tgaaccgctg gcgtcactgg atattccgcg taaacgcgaa
    25201 ctgttgcctt atctgcaacg tctgacacgg gaaatcaaca ttccgatgtt gtatgtcagc
    25261 cattcgctgg atgagatcct ccatctggca gacagagtga tggtactgga aaacggtcag
    25321 gtgaaagcct ttggcgcgct ggaggaagtg tggggcagta gcgtgatgaa tccgtggctg
    25381 ccgaaagagc aacaaagtag cattctgaaa gtgacggtgc ttgagcatca tccgcattac
    25441 gcgatgaccg cgctggcgct gggcgatcag catttgtggg ttaataagct ggacgaaccg
    25501 ctgcaagctg cgctacgtat ccgcattcag gcttccgatg tttctctggt tttacaaccg
    25561 ccacagcaaa ccagcattcg taacgtattg cgggcaaaag ttgttaatag ttatgacgac
    25621 aacggccagg tggaagtgga gctggaagtc ggcggtaaaa cgctgtgggc gcgtatcagc
    25681 ccgtgggcca gggatgaact ggcgatcaaa cctggcctgt ggctgtatgc gcaaattaaa
    25741 agtgtgtcga taaccgcctg attaaatcag gtggctataa atgaactggg caatgctgtc
    25801 ggtggtgtta tcaccaatca caatgttggc gcgcgctttt actgcgtcat cggcgttgcc
    25861 catcgccacg cctgtaccag cggcttccag catactaata tcattgaagt tatcgccgaa
    25921 ggccacgacg ttttccatcg accagccttg cgcctcaacc catttcgtca aacgtttacc
    25981 tttgctgttg ccgccgcgtg caatatcaac ctgatcgtgc caggaccatt cacactccag
    26041 tcccagttca tgttcgacat gcttaccaaa attctgcaat tgcggcaggt catcgtgcgt
    26101 cagggcgaac ttccatacgg cgttaacttg ttgcgccgtt tcagccagag aagcgacttg
    26161 tgtgaaagtc ggacgctgtt ccggcggcag ggtttgcgcc cagttagacg tgcgaatgac
    26221 atgtccggtc gggtgctcat aaaccatcgc atcatccaca tacatcagac cgtgaatgtg
    26281 gtgttcattc agcatctcaa tgagttgcag ggctttgtta acaggcattg ggtccgcttc
    26341 tagtacggtt tttgcatgat aatcatacaa ataggtgccg ttacagcaaa tagcaggtgt
    26401 atccagcgcc agcgcctgat aaaaaggatg aatagcgacg tgatggcgac ctgtgacgat
    26461 gattaatcgg tagcctgctt cgcgagcacg ggccagggct tctatcgatg aaggaagcag
    26521 ggtctttttc ggggtcaata aggtgccgtc taagtcgaga gcaatcacgc gtgtggtcat
    26581 ggcgtattcc agattaaggt taagaatttt cgtctgcgcg aatggtacac cgataccact
    26641 atcggacaaa attctgcgtt ttaattcagc attcaccgcc aaaagcgact aattttagct
    26701 gttacagtca gttgctaaat gcaaaggagc attcatgaag caaacagttt atatcgccag
    26761 ccctgagagc cagcaaattc acgtctggaa cctgaatcat gaaggcgcac tgacgctgac
    26821 acaggtagtc gatgtgccgg ggcaggtgca gccgatggtg gtcagcccgg acaaacgcta
    26881 tctctatgtt ggtgttcgcc ctgagtttcg cgtcctggcg tatcgtatcg ccccggacga
    26941 tggcgcgctg acctttgctg cagagtctgc gctgccgggt agcccgacgc atatttccac
    27001 cgatcaccag gggcagtttg tctttgtcgg ttcttacaat gcgggtaacg tgagcgtaac
    27061 gcgtctggaa gatggcctgc cagtgggcgt cgtcgatgtg gtcgaggggc tggacggttg
    27121 ccattccgcc aatatctcac cggacaaccg tacgttgtgg gttccggcat taaagcagga
    27181 tcgcatttgc ctgtttacgg tcagcgatga tggtcatctg gtagcgcagg accctgcgga
    27241 agtgactacc gttgaagggg ccggcccgcg tcatatggta ttccatccaa acgaacaata
    27301 tgcgtattgc gtcaatgagt taaacagctc agtggatgtc tgggaactga aagatccgca
    27361 cggcaatatc gaatgtgtcc agacgctgga tatgatgccg gaaaacttct ccgacacccg
    27421 ttgggcggct gatattcata tcaccccgga tggtcgtcat ttatacgcct gcgaccgtac
    27481 cgccagtctg attaccgttt tcagcgtttc ggaagatggc agcgtgttga gtaaagaagg
    27541 cttccagcca acggaaaccc agccgcgcgg cttcaatgtt gatcacagcg gcaagtatct
    27601 gattgccgcc gggcaaaaat ctcaccacat ctcggtatac gaaattgttg gcgagcaggg
    27661 gctactgcat gaaaaaggcc gctatgcggt tgggcagggg ccaatgtggg tagtggtcaa
    27721 cgcgcactaa ccgctgattt aaccggcgca gtctctcctg cgccggtgta ttaacctatc
    27781 tcctgtaacg catgtctctg gcgttggacg atattggtcc acaaattgtc tttatcgtca
    27841 gtccacaaat taatcagcaa ggcaaaaaag cgttcagctg ccggagaaag cacggcatct
    27901 ttgcggcgaa taatccccaa tgtccgacgt ataacgggtt ccaccagtgg gataccaata
    27961 atcgaagaat agggcgcatg gggcattgcc agaccaggaa gcgccgaaat ccccagtccc
    28021 gcctccacca gccctaatga cgtcgaaaga tgacgcactt cgtaaaacca gtccagcttc
    28081 cagggcttgt cggctagctg ttgttctatc aatagtcggt tgccgctgga ggagcgtacg
    28141 ccaatcattt tgtaacccac taactcctgc cattctacaa gctgcttttt ggccaatgga
    28201 tggtcacgcc gacaggccag cacgaacggc tcgttgacca gtggggtaaa atcaatggat
    28261 gaatttgtga cgttgttcat gtttatgcca aagtctgatt cgttacacag caccgattcc
    28321 atgcaattat tcgtgccttg ttccagaatc cgcactttaa tattgggata tagctcatta
    28381 aatttaccga ttgccaatgg taaaaaataa aatactgcgg tcggaataca tgctaacgtc
    28441 accataccac gatgataagc attcatatca cgaatattaa aaagtgtttc atcgaatttt
    28501 tttattaatt cccgcgcctc cgggagcaac ctttttcctg cttttgttaa cgtaaccttg
    28561 cgagttgtac gctcaaatag ttgaatgtgt aaatcttcct ccatcttttt tattcttcgc
    28621 gttaatgcag gttgcgtaat attgagtaat ttagcagcat tgttaaatga actggactct
    28681 gccagtatga caaatgcctt catacttgat aattcatgct tcattattac tccggaaaat
    28741 ggaagcgacg attttgggtg actggcagtt aaaaatttta actgcattta gccaacttaa
    28801 attaatgaaa aaatgttatt aatcgttgag ctaaagtcat tagagatgct ttgcccttaa
    28861 tgtaaccata tcgcaataag ttatgttttt aaattgaggg cattattatg gaaaaaatac
    28921 cctgcgtgat gatgcgaggt ggaacctcga ggggcgcgtt cctgttagcg gaacatctac
    28981 ccgaagatca aacgcagcgc gataaaatat tgatggcaat tatgggttcc ggtaacgatc
    29041 tggaaattga cggtattggc ggcggtaatc cgctgaccag taaagtcgcc attattagcc
    29101 gctccagcga tccgcgtgca gatgtcgatt atctgtttgc tcaagttatc gtccatgagc
    29161 aacgtgtcga taccacgcct aactgcggca atatgctgtc tggcgttggg gcattcgcca
    29221 ttgaaaatgg tttgattgca gcgacttcgc cagttacccg cgtacgtatc cgcaacgtca
    29281 atacgggtac gtttatcgaa gctgatgtgc aaacgccaaa tggtgttgtc gagtacgagg
    29341 gtagcgccag aattgacggc gtaccgggta ctgccgcacc ggttgcgctc actttcctga
    29401 atgccgccgg aacgaaaacc ggaaaagtct tcccgacgga taatcagatt gattattttg
    29461 acgatgtccc ggtgacctgt atcgatatgg cgatgccagt tgtcattatt cctgctgaat
    29521 atctgggaaa aacaggctat gaattaccgg cagagctgga tgccgacaaa gcattattag
    29581 cccgcattga atctatccgt ctacaagcgg gtaaagcaat gggcttaggc gatgtcagta
    29641 atatggttat ccctaaacct gtacttattt ctccagcgca gaaaggcggg gcaattaatg
    29701 tgcgttattt tatgccgcat tcttgccatc gcgctttggc gataaccggt gctattgcta
    29761 tttccagtag ttgtgcattg gaaggcaccg tcacccgaca aatcgtccct tctgtaggat
    29821 acggcaatat caatattgaa caccccagtg gtgcgctcga cgttcattta agtaatgaag
    29881 gtcaggatgc cacgacgtta cgcgcatctg ttattcggac gacaagaaaa atattttccg
    29941 gtgaagttta tcttccctga aaaa
//
LOCUS       DAZGJT010000053        29649 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZGJT010000053 DAZGJT010000000
VERSION     DAZGJT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.053"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..675
                     /locus_tag="ACHKFK_002619"
     CDS             <1..675
                     /locus_tag="ACHKFK_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO9052925.1"
                     /translation="IERLVKDTCLNKKTVQAGLISLMKMGLISDTGERKGATKRVRVF
                     SLNITKNGNIKGNREGGNEPENGNVTENGNIPKNGMLNDPKNGIQNQSYNQSFNQERE
                     SRTKSGDSVPHDPGANNAVMNNFVSPGGPGQLGKFVMHEQWQPSDDFLRKSSLQGIYL
                     DSLPTAQELAEFRIYWMAEGKAYHQAQWEQKLARRLQISRQKQSTLPDNNVPHWNSPE
                     AWEDFL"
     gene            672..1457
                     /locus_tag="ACHKFK_002620"
     CDS             672..1457
                     /locus_tag="ACHKFK_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9052926.1"
                     /translation="MNNVFTAIQNRDGEALSRMSGYEHQYVNNDNVVNMSAERLVDAL
                     FKQLKQLFPAAVVTNLKTPEQEVAAKQQWIAAFAEGGIRTREQVSAGMRHARASESPF
                     WPSPGQFIKWCKDSKMVLGVTIDDVMAEFHRYSKEKSLYPGGPERFPWRHPVMYWVVC
                     DTRRAMYQRQLSEIEVEKHARRLLDDWAKKVASGQQIPDPVISIQAKSEPMSTPPDTG
                     RDVYHPLGRSFGCMPNAATLGGITPAQWLMEEYRRGKEAGFIK"
     gene            1575..1919
                     /locus_tag="ACHKFK_002621"
     CDS             1575..1919
                     /locus_tag="ACHKFK_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HFO9052927.1"
                     /translation="MRKQIQALGRLKTGQMNKTESAYCQHLELRKRAGEIVWYRFEGI
                     KLRLADNTFYTPDFAVMLATGEMELHEVKGFWTDDARVKTKVAADQYPFRIIGVTVKP
                     KKAGGGWKIEEF"
     gene            1981..2433
                     /locus_tag="ACHKFK_002622"
     CDS             1981..2433
                     /locus_tag="ACHKFK_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052928.1"
                     /translation="MEINIKGLVAAGHEMTSELKAECGAVDMRSVAKLISDLATQLEV
                     QLVRANALAAENAGLKSGAMDEIKVINRGGQAYCVKDGVRVNPMYARGWNDYRAKSLQ
                     SDTPATDAFLAEIRAPGVEMFADNLLCPDLDGTIRDFAAQLRKGGGQK"
     gene            2430..2747
                     /locus_tag="ACHKFK_002623"
     CDS             2430..2747
                     /locus_tag="ACHKFK_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052929.1"
                     /translation="MTITKQRVEEIISRIEMYGHGAGYTAEEVYDLAVLALNLSNIAN
                     LKRYELDMGGCDSCGQDCGADMTEDSDGDYVLFDDVVKLFEFDTTTQKLEGPAKETTS
                     ERN"
     gene            2734..3306
                     /locus_tag="ACHKFK_002624"
     CDS             2734..3306
                     /locus_tag="ACHKFK_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9052930.1"
                     /translation="MSEIDYQALREIAKQATQGEWVAFISSGTGTYAVHTPGDKRCED
                     VIKWTGFDGQNNAENNARYIAAFNPEVVQALLDERERNQQYIKRRDQENEDIALTVGK
                     LRVELEAAKSKLNEQREYYEGVISDGCKRIAELEAREVQLPTRYDLRYGHPINADERQ
                     VMIPKENGSWLYLIDLEHALRVADIRIKGE"
     gene            3308..3486
                     /locus_tag="ACHKFK_002625"
                     /pseudo
     CDS             3308..3486
                     /locus_tag="ACHKFK_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3820..4404
                     /locus_tag="ACHKFK_002626"
     CDS             3820..4404
                     /locus_tag="ACHKFK_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9052931.1"
                     /translation="MITITKGRLLTIKQWRETYGPGSNVVLPAEEAEELARIALASLE
                     AKPIGAFHIAEQQVDGTSDYLKDGEWPIDNGIIEVYAAPPIPVVPEEKPMPNPLKMYA
                     VDAVAAIAEVRGWNACRAAMLQSQGNCIKDGWISCSEQMPDKLIPVMVMYEDGEMWSA
                     MWNGNRWDDGTEYPDPHSVTHWREMPAAPKQEVK"
     gene            4404..4526
                     /locus_tag="ACHKFK_002627"
     CDS             4404..4526
                     /locus_tag="ACHKFK_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052932.1"
                     /translation="MNKCNALLYAMVIGFGLAAGIRVYIAWESLINLAWSAIRG"
     gene            4519..4857
                     /locus_tag="ACHKFK_002628"
     CDS             4519..4857
                     /locus_tag="ACHKFK_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052933.1"
                     /translation="MAKSPAERKAAQRARQSAAGERKIELVLDKQEQEMLARNCAARR
                     PGRDPYEMAEYIALLIRQDDARVRGRIKSISRKRCGKCGERVPVNSCPCNGDSQCWVT
                     KGWHETKLIV"
     gene            4898..5572
                     /locus_tag="ACHKFK_002629"
     CDS             4898..5572
                     /locus_tag="ACHKFK_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001090114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9052934.1"
                     /translation="MNTAANYGGFILHGTITTTVTITTVVMMPAEHKTYKRKSTQFKP
                     LTAMQEAYCQSYIKTPENQTQAAINAGFSPNTAAVKASVMMRDERIQKRIAELMEERN
                     KRMRVSADYVLMRLVEIDQMDVIDILNDDGSLKPIREWPKIWRTTLSGFDLSSTIMNM
                     NEDSIETILKKIKWPDKVKNLEMIGKHVDVNAFKERLDVNVNVTIADRIAAARKRLKE
                     RQDGNQ"
     gene            5569..7044
                     /locus_tag="ACHKFK_002630"
     CDS             5569..7044
                     /locus_tag="ACHKFK_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9052935.1"
                     /translation="MTDTALSPEEQLIEDIAGFTHDPLGYALYAFPWGEEGTELAHAT
                     GPRKWQADAFREIRDHLQNPETRYQPLMLARASGHGIGKSAFISMLINWGMSTCEDCK
                     VVVTANTDNQLRTKTWPEIIKWSNLAITKYWFTCTATAMYSNDPGHDKRWRADAIPWS
                     EHNTEAFAGLHNERKRIIVVFDEASNIADLVWEVAEGALTDEDTEIIWVAFGNPTRNT
                     GRFRECFRKYKHRWKTAQIDSRTVEGTNKQQLQKWVDDYGEDSDFVKIRVRGIFPDAS
                     ELQFIPTGLTDEAMKRVVTAAQVAHAPVIIGVDPAYSGVDDAVIYLRQGLHSKVLWTG
                     NKTTDDLIMAKRIADFEDQYQADAVFIDFGYGTGLKSIGDGWGRTWQLVPFGGASTDP
                     QMLNKRGEMFNACKTWLRLGGMLDDQETADDLSAAEYKVRVDGKIVIEPKEDIKERLG
                     RSPGKGDALLLTFAFPVSKRLRIPGQQNQQGKAITDYDPYA"
     gene            complement(7135..7506)
                     /locus_tag="ACHKFK_002631"
     CDS             complement(7135..7506)
                     /locus_tag="ACHKFK_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp49 family protein"
                     /protein_id="HFO9052936.1"
                     /translation="MNDQQIEKEIVEKGKTAPRVTPDHIGSIIAQEAYFTAEDGAFGK
                     AIKAKHTGGEVNYQPHESLSLLTFCVLVLRNGFTVTGESSCASPENFDAEIGRKIARQ
                     NAVNKIWMLEGYLLKQKLSEQ"
     gene            complement(7705..7781)
                     /locus_tag="ACHKFK_002632"
     tRNA            complement(7705..7781)
                     /locus_tag="ACHKFK_002632"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7745..7747),aa:Arg,seq:tct)
     gene            8209..8415
                     /locus_tag="ACHKFK_002633"
     CDS             8209..8415
                     /locus_tag="ACHKFK_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052937.1"
                     /translation="MCMGSSPSVPATPEVQAAPQEQDAAVVDARDEETRRRRAAAGRS
                     STLLTGSQGDTSTANTSGKTLLGQ"
     gene            8430..10108
                     /locus_tag="ACHKFK_002634"
                     /pseudo
     CDS             8430..10108
                     /locus_tag="ACHKFK_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
     gene            10105..10401
                     /locus_tag="ACHKFK_002635"
     CDS             10105..10401
                     /locus_tag="ACHKFK_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052938.1"
                     /translation="MTDYEDDQLKEENARKQRDMAQREIDDIRFVMSSEQGRRVVWSV
                     LEKGRVFSAISPMDAMAMAFNEGQRNLALELFQRVMAHCPEQYLKMAKEASEQE"
     gene            10404..11099
                     /locus_tag="ACHKFK_002636"
     CDS             10404..11099
                     /locus_tag="ACHKFK_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFO9052939.1"
                     /translation="MNLFERLLYRRLCNEQPVDGGAAPAASEPSAPAGDNPAPVGDPS
                     QQEGDKPQPVADGDKPADDKKPENDKQDEKKDGNKPEGAPEKYEFQAAEGVELDTEAL
                     KEFEPVARELNLTNEQAQKLVDAYPKILAGVQQRQAEAWQKTTEQWAADVKADKEIGG
                     DKLISNLSAAQRALDQFGTPELKEYLNTTGLGNHPDLVKTFVKIGKAMSEDGMVTGGN
                     EGQRSAAEVLYGK"
     gene            11114..12100
                     /locus_tag="ACHKFK_002637"
     CDS             11114..12100
                     /locus_tag="ACHKFK_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9052940.1"
                     /translation="MAVKGLTALTLADWGKRVDPNGKVDKIIELLGQTNPILQDMPFV
                     EGNLPTGHRTTIRSGLPSATWRLLNYGVQPSKSTTVQVTDSVGMLETYAEVDKSLADL
                     NGNTAEFRLSEDRAFIEAMNQQMAQTLFYGDSSVNPQQFMGLSSRYSSLSAGNAQNII
                     DAGGTGTDNTSIWLVVWGENTVHGIFPKGQKAGIQMEDKGQVTLEDVNGGKYEGYRTH
                     YKWDNGLALRDWRYVVRIANIDVSNLSEPSSAANIAKLMVKALHRIPNRGMGRPVFYM
                     NRTVGQALDLQSLEKTSLAISVKETEGEWWTSFRGVPIRETDALLETEARVV"
     gene            12152..12589
                     /locus_tag="ACHKFK_002638"
     CDS             12152..12589
                     /locus_tag="ACHKFK_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bbp16 family capsid cement protein"
                     /protein_id="HFO9052941.1"
                     /translation="MITDKLLMFSEAQAVTNTAASTDVIDLGPIDGKRRDIGVGYPLE
                     FWALVNTAAAASGDATVNIQLQTSENNSSWTTIYDSGALAKTALTAGKRVVSAKVPAG
                     VQRYLRVNYSVATGPLTAGEFTAGISLDVDANTPYPIRSKVTG"
     gene            12600..12935
                     /locus_tag="ACHKFK_002639"
     CDS             12600..12935
                     /locus_tag="ACHKFK_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052942.1"
                     /translation="MSGEKPRYRVLRLSHIHNTLWPEGAEIEYEGEPGSALEPVNDAA
                     RQAKAKVAGKVSMAATSTKIINDVSDDGELDKLREEYELLFNEKPHHNAKAETLREKI
                     ADKRKELGV"
     gene            12986..13309
                     /gene="gp10"
                     /locus_tag="ACHKFK_002640"
     CDS             12986..13309
                     /gene="gp10"
                     /locus_tag="ACHKFK_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid staple protein"
                     /protein_id="HFO9052943.1"
                     /translation="MELVNLKTGTDSYQDESGETRTRDEYPWGLCITLNNDTLNKLKA
                     QPQGVGTEVMITAKAVIRGLSARETDDGVNRSADLQITDMAIAPVSGDVEKSAAETLY
                     GNGGE"
     gene            13309..13914
                     /locus_tag="ACHKFK_002641"
     CDS             13309..13914
                     /locus_tag="ACHKFK_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052944.1"
                     /translation="MASVVEICNRALSNIGNSRSINSLTEASKEAGECSLHFEACRDA
                     VLSDFDWNFATKRVALADTSNPPPDWEYAYQYPSDCLRITEIMLPGVRNPTAAMRVQY
                     EVGADTNGTGKLIYTDQPQAWLKYVSRVSDVNMFDAIFMEALAWRLAAAINMALTGNA
                     DLGTFALNMYNREILSAGSHSQNESQEPQPPVDEFTIARLS"
     gene            13914..16385
                     /locus_tag="ACHKFK_002642"
     CDS             13914..16385
                     /locus_tag="ACHKFK_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052945.1"
                     /translation="MAISWIQPSFAGGEIGPSLYGRIDMAKYQVALRKCDNFIVRQYG
                     GVENRPGTRFVGAAKYPNRKCRLIPFQFSTVQTYALEFGHQYMRVIKDGALVLNSSNV
                     IYEIATPYTEADLFRIKFTQSADVLTLVHPAYPPKELRRYAHDNWQLVDVVTKNGPFE
                     DINIDESVTVYASASTGTITLTASASIFGVEQVGKLFYLEQPAVDSVPVWETSKSTSI
                     GDIRRADSNYYRAVTAGKTCTLRPSHTEGTSWDGWGGSGDDDTGIEWEYLHSGFGIAR
                     ITAANGTTATAEVISYIPSQVVGANNASYKWAKYAWNSVNGYPGTVVYYQQRLYFAAS
                     TAFPQTIWASRTGDYKDFGKSNPTQDDDRIIYTYAGRQVNEIRHLIDVGSLVALTSGG
                     EYVITGDQNKVLTPSSFAFSSQGSNGSSNVPPIAVANIALFVQEKGSVVRDLAYSFDV
                     DGYQGNDLTILANHLFQKHSIVDWCFSIVPYSSAFCIRDDGKLLVMTYLRDQQVFAWA
                     PQSSTGKYESTCSISEGNEDAVYFVVNRTVNGQTVRYIERLSSRLFTSDEDAFFVDSG
                     LSYDGRNTSDRTMTITGGSGEWDYRTEYTISVSGGAYFTSSDVDAQLQFPYTGTDPDT
                     GDEVSKELRCDIISVTSNTAVVVRANRNVPPSLRNAATTNWQMARRTFGGLSHLEGQT
                     VNILSDANVEPQKVVSGGAVTLESPGAVVHIGLPITSEFETLDININGQETLLDKKQV
                     IPSVTLVVNASRGIWATTPGGKWYEYPQREFEFYDDPVDDATGKVEVKLDSNWGKNGR
                     VRIRQLDPLPLSVLAVIPRLTVGGF"
     gene            16385..16849
                     /locus_tag="ACHKFK_002643"
     CDS             16385..16849
                     /locus_tag="ACHKFK_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052946.1"
                     /translation="MIDVRIIPATEEHLQMILPDVRQADIDELYAVSLMTTEDALRVG
                     LRTATMAWSGFANRELVTMFGVSPASMIGGNGTPWLVGTSRIEKYQKTFLRHCRPVLQ
                     QMLAVYPRLENYVDERNHVAKAWLHWLGFRLEEAAPYGALGLNFHRFHMERK"
     gene            16849..17394
                     /locus_tag="ACHKFK_002644"
     CDS             16849..17394
                     /locus_tag="ACHKFK_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052947.1"
                     /translation="MCDPVIAGGAMLAMSGIQAYTQYQQGKYASKVAEANADIATAQA
                     NDAINRGNAEAEQRRRETRQRLGTQAATMGATGADLSTGNALDIFGDTAQFGALDALT
                     TVNNAQREAYGYQVQAANYKAEASSARKQGNVGAATTLLTAPLKAYGAYQMFGGTWSP
                     FTQSTHAPIGAAAGTRLPGGL"
     gene            17394..19907
                     /locus_tag="ACHKFK_002645"
     CDS             17394..19907
                     /locus_tag="ACHKFK_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052948.1"
                     /translation="MPVVPTVSGRQVESRGVQSAGLQTFSQPGIGDAFVRAGTEAIDV
                     LGQAKQRANIALAQEASLNLSQISSDLLNNPETGLLNLKGKNATGKGQEYTQQFDAQV
                     EQLAMSLPDEQARNAFMQQAQQQRIQFTTQAGRHEIGQINAYEEGQFQATLLNNGKNA
                     AALYGDNAAYVLANKQTFQQIEDYGIAHGWSDEQIQAKKIEFKEKVADAALSQWSASN
                     ATAFIQSNGELSDTAAGARRAVADNDSSERARGIRNNNPGNLEYSKTNPWVGQTGDDG
                     RFAKFETPEHGIRALGRNLMSYQRQGIDTVSEIINRWAPPTDKNDTMSYIKAVCEQLG
                     VSADEPLDASNPDTLKALCAAIIHHENGSQPYSDQQLTAGVSAALGLSTIPTNTKRYT
                     GNAAFDAASPEAQASFMRQADQLRRQQQAEYKTMIDSQVRDATAAYMRGVEFPNPPGE
                     DDFIAAYGVREGNLRYTEFKNTQIAGQYIGSFRNMPTSSITAYVEQLRPDTGETGEGY
                     AARAALYDNVVSAANQVIKQRQSDPVQFSLAAGQAKPIDMSNKDNFGQSVALRAAQVS
                     DLAKSYGTPLTFFSKDEANQIGVFFRDAPVSQQAAYLDTIRQSTGGGQVYMSALQQIS
                     ANAPSAAVAGILMDKPGGILAEKNWFNPDVSVSPETAAQTILAGAAARKGTDDAKGIP
                     MPKDADLRLEFSDMVKDAFAGDAQGASMAYEIAKDYYAGVMAKKGVVSGEIDNDIWKQ
                     AVNVATGGVHDYNGMGNVLLPWGMSAEQFDKQVNQAWNEQVVGTGIKTPPGQYGLQSY
                     GDSQYLVKLGTGYLLKDDGSPVVLDLTQKRQRFSGDIPQ"
     gene            19904..21706
                     /locus_tag="ACHKFK_002646"
     CDS             19904..21706
                     /locus_tag="ACHKFK_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052949.1"
                     /translation="MSYFGLNPVNQNQQLDEAASNPAGFNSDVGFFDNAVGAALSGLY
                     SGLVAKPDQLLWAGMDKIVSPIAQFVNENTSLNDTSVSYIAEQRKLAEQQVKRLTPDA
                     ATTGTAGQVLYGLFDMGGQAVVGTTLGGPVGGAAAVTSLQGFSEFERLTAQGVDFRTA
                     QEAGLVQGITAGAGTLIPMSLGLRAGGALAEGVAAQLARTGESSVRRAAATAVRATPD
                     IAYAAGTNIAFGMAQRGLTAKTLRDGGYSEMANQYDVLDRQAIAIDAVLGVAFGGVGR
                     FINSRGESTNAPNFSPVDIDAALAANAAHHAEIDIAPGVPINVLSRNSHIQALRKAMS
                     AVSQGRPVDVASIVESASFSEIPGRKNLLSQAVNEALSSVDDGVTARAIENRLLEEQA
                     AQLLPRGDRQVYQSEIANSQRILDNLTEQRAQILAEEPTGSGKALSRARSDKQARIRD
                     IDQRIRQAQERLEFSRNALAPHEHGGQFFEARAELARRLQVESELNAQAVSFYKTAEV
                     RTPDEVAPFEPDKILQQAEQKMMADQAGDIDLRIAEDSLLESPDMIITVLDDDGNPQS
                     RSAREVLDEANRESEQAIQDSSLFDVAVACFLRG"
     gene            21712..24186
                     /locus_tag="ACHKFK_002647"
     CDS             21712..24186
                     /locus_tag="ACHKFK_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052950.1"
                     /translation="MRQECIQAVQQAAQRTLTAREIQNIEDRIYRNMRSIARDDPMSW
                     RQLSESERLYRAAQLASEELQREAALKKRRVALTIAARQRLDKFINSYQGADGKLGAL
                     NRTIAFNADGKSNFLSVESRTKATRDYALSQLQEAFEAVDPRFFGLFEDEAGVRDLVY
                     EMRGQNTGNAKARKGAKAWREVTDLLRRRFNDAGGDIGYLENWGIPQHHSMEKVGAVS
                     KDKWVSDVIGKLDRKYYIRADGQLMNDAELSAFLGEAYNTIATGGLNKLTDTGMRISG
                     ARANRGNASRQIHFKDADSYLQYQQLYGDRSLWEIMVGHLEGISKDIALVETYGPNPD
                     HVFRSLLDQVKAETATANPSKTGSVERLANKTENLYNFISGKTQPVANPHIARWSDNI
                     RNWLVASRLGSALLSSFSDLGTIYLSAKVTNLPMNQLFRNQLEAMDPTNRTELARARR
                     AGLAMESLLGSVNRWAMDNMGPSVSRWAATAVMRASGLTAWSDAHKRAYGVTMMGSLG
                     EVVSRTPDLRSLDDSDFRILKSKGITDTDWSVWKLAQQEDWGNGNNTMLTPESIMRIP
                     DSAVKHLGEPERVKFEAMRKLLGAVTEEVDMAVITPGAREQMFVGSGLQRGTWKGELT
                     RSVFLFKSFPISVVMRHWHRAMGMPSAGGRAAYIATFLASTTMLGALSMQITDLINGR
                     NPKEMTGDNMVKFWINAFLKGGGAGLYGDFLFSDHTRYGSGALASMLGPVAGLVDDVV
                     KIAQGIPLNAVEGKNEQTGGDLVKLGKGLMPGANLWYLKAALDHMIFNQMQEYFSPGY
                     LRKMEQRSKKEFNQTYWWRPQDVTPQ"
     gene            24196..24609
                     /locus_tag="ACHKFK_002648"
     CDS             24196..24609
                     /locus_tag="ACHKFK_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052951.1"
                     /translation="MKELLLLSVFLISACSTSYDVYDGVDKAYCDKVKMDFSLAKTAK
                     DSCIDHYVKTYTKPASSASDIAEGAVFECNKVISIAASSSYDAAVCAMAERNGMSVQK
                     INSMISSNDEAKIRTDISSVKKDAMNRVVKYQSSL"
     gene            complement(24648..24809)
                     /locus_tag="ACHKFK_002649"
     CDS             complement(24648..24809)
                     /locus_tag="ACHKFK_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000708858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transmembrane anchored protein"
                     /protein_id="HFO9052952.1"
                     /translation="MKKAFAALFVLLSLVASTQAFAGRCQHDSDTAADGSRCGGRSAD
                     SRPGGGGIR"
     gene            complement(24902..25447)
                     /locus_tag="ACHKFK_002650"
     CDS             complement(24902..25447)
                     /locus_tag="ACHKFK_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HFO9052953.1"
                     /translation="MSSSASQNDKNQIVRYKGRVLHTQNFSALCASDLELKKVSDAFT
                     QYWKTGYHPSLGKDAAFARPTEMLKLNVRHTHVDNQDYIPEDSDKKHTGKKSSWDAWK
                     NIASVQVKCIPTSDCFLVYSVNHNRDALVMFFVDADAHNITEQEEFKEAAITISYQFF
                     EKTKTEPMPLEEDLFSDKWKE"
     gene            complement(25749..26459)
                     /locus_tag="ACHKFK_002651"
     CDS             complement(25749..26459)
                     /locus_tag="ACHKFK_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO9052954.1"
                     /translation="MANAQTAIFKFESVNPIRSIIIDGQPWFVAQDVCSALRIQNVTQ
                     SLEKLDDDERSMFNIGHEHRAIFDSRVKEINIISESGLYTLILRCRDAVTPGTIPYRF
                     RKWVTGEVLPQIRRTGSYIKNSLPQEERIKMVADQVANATASAVIQAMKIENKTYSAP
                     LKPGYRSLIHSPSGVLGLTENSLLMNLLNQLQEDGHDVSGAAAELTTMFCYIVGVSKC
                     LRDIQTHAEYINDKAGFF"
     gene            complement(26775..27032)
                     /locus_tag="ACHKFK_002652"
     CDS             complement(26775..27032)
                     /locus_tag="ACHKFK_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052955.1"
                     /translation="MQQYHYPLEDGFTERIHTPGGVRLLVEGSLLMKLLRDLDKDGFN
                     VDGPLAELTALINYVTSSQMSMQDLQTHLDYCAEQLRKQTR"
     gene            27228..29330
                     /locus_tag="ACHKFK_002653"
     CDS             27228..29330
                     /locus_tag="ACHKFK_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052956.1"
                     /translation="MTVSTEVDHNEYTGNGVTTSFPYTFRIFQKSDLVVQVVDLDENI
                     TVLALDTDYTVTGAGGYNGGNVILSKVLANGYLISIYRELPVTQETDLRNQGKFFAEV
                     HENAFDKLTMLIQQVRNWFSLALRKPSFVANYYDAMNNYIRNLRDPSRPQDAATKNYV
                     DGLAETNLSRTLRTPEPISTLPGVEQRKNKIVAMDDSGDPIMVLPESGSATDVMLQLA
                     ATDGFKNIGSCPDVATLRNTEPSSVNQQIIVRGYYSDTPGLGGGVFMSFDSSEADDGV
                     NIFVTPGGKRWKRAGSHIDIPVENGGMMTSCTAEQNSEAFERLTACLPYEGGTLRLNG
                     FYDIKYGAIVPPRVTLEGCGMDSCGLIKTGNDIKTVPDRMWQGVPHSFSKDFIVAVDM
                     DSDTSGDLSGKQTRSTRIIGLSILNTAPNPCDYGIYSAISYNVRLQDLYIHRVKTGYR
                     TSDSWLQTWENITIQDVVTGVKIEAGGTTYNLNNIYVKTCSGVAYDFNNITYSSLTCL
                     AADFVNGTGYKFKDCTGVNLSGCGSEEGTGKIFDINGSRINVTAFRGVNHHDGGVEAV
                     SLESCFISLVSSHFSGYSGVSSSKYIYLNDATANFNGAVIPDNTTDSIRWGSGQSWIN
                     KVDSGGTYSITGNSSWTALCRLESGYITVLSVSPPTGDTTNFPFGTKWELLGPVSTGP
                     WKWTYVSTGEWKVSATTT"
BASE COUNT         7858 a         6671 c         7900 g         7220 t
ORIGIN      
        1 atagaacgcc tcgttaaaga cacttgcctg aataaaaaaa ccgtgcaggc cgggcttata
       61 tcgctcatga aaatggggct tatttcagat accggagaga gaaagggagc gacgaaaaga
      121 gtgcgggttt tctctcttaa tataaccaaa aacgggaaca ttaaaggcaa ccgggaaggg
      181 ggtaatgaac ccgaaaacgg taatgttacc gaaaacggga atatacccaa aaacgggatg
      241 ttgaatgatc ccaaaaacgg gatccagaac cagtcatata accagtcatt taaccaagag
      301 agggagagca ggacaaaaag cggggattct gtgcctcatg accccggcgc aaacaacgcc
      361 gtgatgaata actttgtttc tcctggtggg ccagggcaat taggcaaatt tgtcatgcat
      421 gaacaatggc agccatcaga tgactttctt cggaaaagct cattgcaggg gatctacctg
      481 gacagtctgc caacggcaca ggaacttgca gagttcagaa tttactggat ggctgagggt
      541 aaggcatacc atcaggcaca gtgggagcag aagctggcaa ggcggctgca gattagcaga
      601 cagaagcaat caacattacc tgataacaac gttccgcact ggaacagccc tgaagcgtgg
      661 gaggatttct tgtgaacaac gtttttaccg cgatacaaaa ccgtgacgga gaagcccttt
      721 ctcgcatgtc aggttatgag catcagtacg tcaacaatga caatgtggtg aacatgtcag
      781 cagagaggct tgttgatgcc cttttcaaac agctgaaaca actgtttccg gcggcagtgg
      841 taaccaacct gaagacgcca gagcaggaag tagctgcaaa acagcagtgg attgctgcgt
      901 ttgccgaagg ggggatccga acccgtgaac aggtttctgc tggtatgcgc cacgcccgcg
      961 ccagtgaatc tccgttctgg ccgtcgcctg ggcaattcat caagtggtgt aaagacagca
     1021 agatggttct tggcgtcacc attgacgatg tgatggcgga gtttcaccgg tacagcaagg
     1081 aaaaaagttt atatcctggt ggtcccgaaa gattcccgtg gcggcatcct gttatgtact
     1141 gggtcgtatg tgatacccgc cgtgcaatgt atcagcgcca gcttagcgag attgaggttg
     1201 agaaacacgc gcgcaggctg ctcgatgatt gggcgaaaaa ggtggcttcc ggacagcaga
     1261 tacccgatcc ggtgatcagc atacaggcaa agtcagagcc catgagtaca cctccggaca
     1321 cagggagaga cgtttaccat ccactagggc gaagtttcgg gtgcatgcct aacgccgcca
     1381 cccttggggg aataacaccg gcgcagtggc tgatggagga atacaggcgg ggaaaggagg
     1441 caggatttat caagtaatac cagcgcgata gcgcattttt ttacgcctta gcaattacct
     1501 gttaggtaat aaaatattct aaattctatt gatttcgtgt cttatgtggt ttttaattac
     1561 ctcaggggta aatcatgaga aaacagatac aggctcttgg tcgactcaaa acaggccaga
     1621 tgaacaaaac agaatctgcg tattgccagc accttgagct gcgtaaacgt gcaggagaaa
     1681 tcgtctggta tcgatttgag ggtatcaagc tgcggttagc tgacaacacg ttctatacgc
     1741 ccgattttgc tgtgatgctc gccactggcg agatggaact gcacgaagtg aaaggtttct
     1801 ggaccgacga cgccagggtg aaaaccaaag tcgccgcaga tcagtatccg ttccgaatca
     1861 tcggggtaac ggttaaacca aagaaagcag gtggtggctg gaaaatcgaa gagttctgaa
     1921 tcgacgatct ttttagttat caatgtaatc aataagttat gtggataagc gagggtaaag
     1981 atggaaatta atatcaaagg gttagttgcc gccgggcatg agatgacttc ggaactgaaa
     2041 gctgaatgtg gtgccgttga tatgcgcagt gtggcaaagc tgatcagcga tttggcaacg
     2101 caactggaag tgcaactggt gcgtgctaat gcgctggctg ctgagaatgc ggggctgaag
     2161 tctggcgcta tggacgaaat caaggttatc aaccgtggag gtcaggcata ttgcgtaaaa
     2221 gatggagtgc gagttaatcc catgtatgca agagggtgga atgactatcg cgcaaagtct
     2281 ctgcaatcag acaccccagc caccgatgct ttcctggctg aaattcgtgc accgggggtt
     2341 gagatgtttg ctgacaatct gttgtgtcca gaccttgatg gcactatccg tgactttgct
     2401 gcccagcttc gcaaaggagg agggcaaaaa tgacaatcac aaaacaacga gtagaagaaa
     2461 tcatatcgcg cattgaaatg tatgggcatg gtgcagggta taccgccgaa gaggtttatg
     2521 accttgctgt actggcgctg aatttatcaa atatcgcaaa cctgaagcga tacgagcttg
     2581 atatgggagg ttgcgactcg tgcggtcagg attgtggcgc tgacatgact gaagattctg
     2641 atggcgatta tgtcctgttt gatgacgtgg ttaagttgtt tgagtttgat acaaccactc
     2701 agaagttaga aggcccagca aaggagacaa ccagtgagcg aaattgacta tcaggcactg
     2761 cgtgagatag caaaacaggc cacgcagggc gaatgggtcg catttatttc gtcgggtact
     2821 ggtacatatg cggtgcatac acctggtgat aaacgatgtg aagacgttat caaatggacc
     2881 ggctttgatg gacagaacaa cgcagagaac aacgctcgtt atatcgcagc tttcaaccct
     2941 gaagtagtgc aggcgctgct ggatgaacgg gaaagaaacc agcaatacat caaacgccgt
     3001 gaccaggaga acgaggatat tgcgcttacg gttgggaagc tgcgcgttga gcttgaagca
     3061 gcaaaatcaa aactcaacga gcagcgcgag tattacgagg gagttatctc tgatgggtgc
     3121 aagcgtattg ctgaactgga agcgcgggaa gttcaattac cgactcgcta cgaccttcga
     3181 tatggacacc cgataaatgc agatgagcga caagtcatga tacctaaaga aaatggcagt
     3241 tggctttacc tgattgacct agaacacgca ttacgcgtcg ctgacattcg catcaaagga
     3301 gagtgatatg gcgttaacac accacgaact ctgtcagatt gcgtacaagt tccttaagcg
     3361 caacgggttc aaggtttgct ttcatgaccg ctttgttgct gtaaccagta ccggagaaca
     3421 gccagatgct atgggattca gaaattcagc atcatgcctg atagaggcga agtgttctcg
     3481 tgctgacttg ttggcagata gaaaaaagcg tttccgtaaa aatccctcac ttggcatggg
     3541 cgactggcga ttctttatta gtgagccgga aattatttca gttgaggatt tacctcccgg
     3601 ctggggatta cttcacgttg ttaacggaag agtacggaaa gtacatggat ggcccagggg
     3661 taattgctgt tggggtaatc ctgacgataa gccatttacc gggaataagc aggttgaatg
     3721 cgattacatg ttatctgcat taaggcgcat ggagttgaga gggcacctta atgaaatata
     3781 tgacggtgtg attgttaata agaaagaagg aaacgcggca tgatcactat taccaaaggg
     3841 cgactgctga caatcaagca gtggcgcgaa acatacggac cgggtagcaa cgttgtactg
     3901 ccagcagaag aagcggaaga actggcacgg attgcactgg catcattgga agcaaaacca
     3961 ataggtgcat tccacattgc agaacagcaa gttgacggca caagtgacta cctcaaggat
     4021 ggagaatggc ctattgataa tggaattatt gaggtctacg ccgccccacc cattccagta
     4081 gtacctgaag aaaaaccaat gcctaaccct cttaaaatgt acgcggtcga tgctgttgcc
     4141 gctattgcag aggtgagagg ctggaacgcc tgtcgtgccg ctatgcttca gagtcaaggt
     4201 aattgcatta aggatggttg gataagctgt agtgagcaga tgccagataa gttaattccg
     4261 gtaatggtca tgtatgaaga cggtgagatg tggtctgcaa tgtggaatgg caatcgctgg
     4321 gatgatggca ccgaatatcc ggatccgcac tcagttacgc actggcgtga aatgccagca
     4381 gcgccgaaac aggaggtgaa gtaatgaaca agtgcaacgc tctgctttat gccatggtga
     4441 ttggtttcgg cctggctgct ggtatccggg tttatattgc ctgggagtca ttaatcaatc
     4501 tggcgtggag tgcgattcgt ggctaaatcc cccgcagaac gcaaagccgc gcagcgcgct
     4561 cggcagtccg ccgccggtga gcgcaaaatt gaactggtgc tggataagca ggagcaggaa
     4621 atgctggcgc ggaactgcgc cgcccggcgc cctggtcgcg atccctatga aatggccgag
     4681 tacatcgcgc tgctgatccg ccaggatgat gcacgcgtgc gcgggcgtat aaaatcgatc
     4741 agcagaaaac gttgcggtaa gtgcggcgag agagttcctg tgaattcatg cccgtgtaat
     4801 ggtgactcgc aatgctgggt gaccaaaggc tggcatgaaa cgaaattaat agtgtgacat
     4861 gtcacgagta gattatgcat gatgaatttg atgggttttg aatactgccg ccaactatgg
     4921 cggctttatt ttgcatggta ctattaccac aacggtaact attaccacgg tggttatgat
     4981 gcctgctgaa cataaaacct ataaacgcaa atcaacgcaa tttaagccac taacagcaat
     5041 gcaggaggct tattgccagt catacatcaa aacgcctgaa aaccagactc aggcagcgat
     5101 taacgcagga ttctccccaa atacagcggc agttaaagcc agtgtcatga tgcgcgatga
     5161 acgcattcaa aaacggattg ccgagttgat ggaggagcgc aacaaacgaa tgcgcgtcag
     5221 tgctgattac gttctcatgc gcctggtgga gatcgaccag atggacgtga tcgacatcct
     5281 caacgacgat gggagcctta aaccaatccg tgagtggccg aaaatctggc gcactacgct
     5341 tagtggcttt gatctgtcat cgaccatcat gaacatgaac gaggattcga tagagacaat
     5401 cctcaaaaaa attaaatggc ctgacaaggt gaagaacctt gagatgattg gtaagcatgt
     5461 tgatgtcaac gcgttcaaag aacgtctgga tgttaatgtg aatgtgacaa ttgctgatcg
     5521 catagcagca gccaggaagc gactcaaaga acgtcaggat ggtaatcagt gacagataca
     5581 gcgttatctc ctgaagagca gttgatcgag gatattgcag ggttcactca cgatccgctt
     5641 ggctatgccc tctatgcgtt cccgtggggg gaagagggga ctgaattagc acatgccacc
     5701 ggtccacgta agtggcaggc tgatgcgttc cgagagatac gtgatcacct gcagaatcca
     5761 gagacgcgct atcagccgct tatgctggca cgtgcttcgg gtcacggtat tggtaaatcc
     5821 gcattcatct caatgctgat caactggggc atgtccactt gcgaggattg taaggtcgtg
     5881 gtgaccgcca acaccgacaa ccagctacga acgaagacct ggccggaaat tatcaagtgg
     5941 tcgaaccttg ctatcacgaa atactggttt acctgtactg ctaccgcgat gtacagcaat
     6001 gaccctgggc atgacaagcg gtggcgagct gacgcaatcc cctggtctga gcacaacact
     6061 gaggcattcg ccggactaca caacgagcgc aaacgcatca tcgtggtatt cgatgaagcg
     6121 tcgaacattg ccgatctggt gtgggaggta gcagagggtg cgctgacgga cgaagacacc
     6181 gaaatcatct gggtggcgtt cggaaaccct acacgtaaca ccgggcgttt tcgcgaatgt
     6241 ttccgcaaat ataaacaccg ctggaaaact gcgcagattg acagccggac ggtggaaggc
     6301 actaacaaac agcagttgca gaaatgggtt gatgactacg gggaagacag cgacttcgtt
     6361 aaaatccgtg tgcgcggcat attcccggat gcatctgaat tgcagtttat ccctaccggc
     6421 cttactgacg aggcaatgaa acgggtggta accgctgcgc aggttgcaca tgctccggtg
     6481 ataatcggcg ttgacccggc atactccggc gttgatgacg ctgtgatata cctgcggcag
     6541 gggctgcaca gtaaggtgct atggactggt aacaagacca ccgacgatct gattatggcg
     6601 aagcgtatcg ctgactttga agaccagtat caggctgacg cagtgttcat cgactttggt
     6661 tacggtaccg gtctgaagtc aatcggtgac ggctggggtc gtacatggca acttgttccg
     6721 ttcggcggtg cgtccactga cccgcagatg cttaacaagc gtggggagat gttcaatgca
     6781 tgcaagacat ggctgaggct cggcggcatg ctggatgacc aggaaacagc ggacgacctg
     6841 tcggcggcag agtacaaagt tcgagtggac ggtaaaatcg ttatcgaacc gaaggaagat
     6901 atcaaagagc ggcttgggcg ttctcctggt aaaggcgatg cgctactgct gacgtttgcg
     6961 ttccctgtgt cgaagcgtct gcgaattccc gggcagcaga accagcaagg caaggccatc
     7021 acagattacg atccctatgc ttaatcctct ggtggggata atgtcgttga tatcctctgg
     7081 tgaggataaa acaaagccag ctcataggct ggctgtttgt gacatgtcac ggtgttattg
     7141 ctcgcttaac ttctgcttca gcaagtaacc ttcaagcatc cagattttgt ttacagcatt
     7201 ctgtcgggca atcttccgac caatttctgc atcaaagttt tccgggcttg cacaggaact
     7261 ctctccggtg acggtgaagc cgttgcgcag caccaggacg cagaacgtca gcagagaaag
     7321 tgattcgtgc ggctggtagt ttacctctcc gccagtatgt ttcgctttta tggctttgcc
     7381 aaaggcacca tcctctgctg tgaaatatgc ctcctgagca ataatgcttc cgatatggtc
     7441 tggcgtaacg cgcggagcag ttttgccttt ctcaacgatt tctttttcga tttgctggtc
     7501 gttcataatc tcaccttaaa aaaatgcccg gcgaaccggg cgaactggaa gcaatgagtt
     7561 atgccttccg tggctgtacg ggtttacagc atgaagtcat cgcaatggcg tcctgctgta
     7621 aaaagggcgg tgatagtcct tcaagggaaa ccatcaccgc caagcccctg gaacttctgg
     7681 catcacggtc cttaggcgtg attctggcgt ggcatgcagg attcgaacct gcgaccaacc
     7741 gcttagaagg cggttgctct gtccaactga gctaatgcca caacgctgag agcacttagc
     7801 ctgttaaggc gccacacttt gtcgcggctc cataaatgct ctcatcgttg taccctcgtc
     7861 tcttccgagg cgtcacaccg aatcgccggg atggtgaatc cccgtgcgcg gaataaaacc
     7921 gctcgacttg cacattccgg ctacctggtt cgtttgctcg agcaagggag ggtgcccctt
     7981 aaacgtatcc agaccgctat cggcgcatgt gccatacgcc gtactgctca aaataaaagc
     8041 tcactccacc tgttcaattt aacgacaagc cagtcaggtt aataaccgga atgaactctt
     8101 tgcttacctg aaaggtaata attcgtgcgt taaatgtcaa ctatctacga taaataaatc
     8161 atatgtggtt aaattggtaa taatttaatt gcgtacggag tcattgatat gtgcatgggt
     8221 agctcaccat cagtgcctgc aacaccagaa gttcaggcag caccacagga gcaggatgcc
     8281 gccgttgttg atgcccgcga cgaagaaaca cgtcgccgtc gcgctgctgc tggtcgtagt
     8341 tctacgctgc ttaccggttc tcagggcgac acatcaaccg ctaataccag cggtaaaacg
     8401 ctgcttggtc agtaaccgga gtcattgaaa tggcggaaac aactaaagag cgattgaaca
     8461 aacagttcgc acaacttgaa agcgagcgtc agtcgttcga gccgcactgg cgcgagttga
     8521 gtgattacat caacccgcgt ggttcccgct ttctgacttc tgaggtcaac cgtaacgatc
     8581 gacgcaatac acgcattatt gattcgaccg ggactatggc ggcgcgcact ctcgccagcg
     8641 gcatgatgtc aggcatcaca agccccgcgc gtccgtggtt tcgcctggct acgccagatc
     8701 ctgaaatgat ggattatggc cctgttaagt tgtggcttga ggggtgcaga accgcatgaa
     8761 cgatatgttc aataagtcga atctctatca gtcgctgccg cagttatacg gaagcctcgg
     8821 cacatacagc accggtgcaa tggcggtact ggaggatgac gaggacatca ttcgcacaat
     8881 gccattcccg ataggcagtt actacctggc taactcacct cgtggcagtg tggacacctg
     8941 ttttcgcaag ttctctatga ctgttcgtca gcttgttcag gagttcgggc taaataacgt
     9001 cagcgaatcc gtaaaaagca tgtgggaaag cggtacctac gagaagtgga ttgaagtgat
     9061 gcattcggtt tacccgaaca ttgaccgcga tacatcgaag ctggatagca agaacaagcc
     9121 attcaaatcg gtttattacg aggttggtgg cgataacgac aagttgttgc gtgagtcagg
     9181 attcgatgag tttccaatta tggctccgcg ctgggaagtt aacggcgaag atgtttatgg
     9241 atcatcatgc ccgggtatgc tggcgcttgg acctgttaag gcattgcagc ttctccagaa
     9301 gcgcaagtcg cagttgattg ataaagccac caatccgccg atggttgctc cgacttccct
     9361 caagaatcag cgcgcctccc ttcttcctgg cgacatcacg tatatcgatc agattactgg
     9421 tcaggatggc ttcaggcctg cttatctggt taaccccagt acagcagatc tggtagcaga
     9481 cattcaggac actcggcaaa tcattaacag cgcctacttt gtcgatctgt tcatgatgtt
     9541 gcagaacatc aatacccgct cgatgcctgt tgaagcggtg atcgaaatga aagaagaaaa
     9601 acttctgatg ttggggccgg ttctggagcg tctgaacgac gaatgtctta atcctctcat
     9661 tgaccgcgct ttctcgatga tggtgcgtaa aaacatgttg ccgccaccgc ctgacgcgat
     9721 ggaaggcatg cccctgaagg tcgaatacat ttccgtcatg gctcaggctc agaagtctat
     9781 cggcctgtcc agtctggcgt ccacggttaa cttcattggt caacttgcgc aagcgaaacc
     9841 agaagctctc gacaaactca acgttgatca ggcgatcgat gcattcgctg atatgtccgg
     9901 agtgtctcca accgtcattg ttccgcagga acaggttgag caggctcgcc agcaacgggc
     9961 acagcagcaa cagcagcaac aaatgatggc gatggggatg gcggcggcac agggtgccaa
    10021 gacgctaagc gaagctaaaa cttcggatcc gagtgttttg tcagctatgg cgaatgcagt
    10081 tagtggtcag ggtgggcaat cacaatgaca gattacgaag acgatcaact gaaagaagaa
    10141 aacgcccgta agcaacgtga catggcgcag cgtgaaattg atgacattcg ctttgtcatg
    10201 agcagtgaac aggggcgtcg cgttgtctgg tcggtgctgg agaaaggccg tgtgttttcc
    10261 gctatctcac cgatggacgc tatggcaatg gcatttaatg aggggcaacg caatctggcg
    10321 ctggaactgt ttcagcgcgt tatggcgcat tgccctgaac agtatttgaa gatggccaaa
    10381 gaggccagtg aacaggagtg atcatgaatt tatttgagcg tttgctgtat cgccgtcttt
    10441 gcaatgagca accagtcgat ggtggagcag ctccggctgc gtcagaaccg tcagcgcctg
    10501 caggtgataa ccctgctcca gttggtgatc catcacaaca ggaaggtgat aagccacaac
    10561 ctgttgctga tggcgataaa cctgctgatg acaaaaagcc tgaaaacgat aagcaggatg
    10621 aaaaaaagga cggcaataaa ccagagggtg cgcctgagaa gtacgagttt caggctgccg
    10681 aaggcgtaga gctggataca gaagcgttga aggaattcga gccggtggcg cgagaactta
    10741 acctgaccaa cgagcaagcg caaaagctgg ttgatgctta tccgaagatt ctggcaggtg
    10801 ttcagcagcg ccaggcagaa gcctggcaga aaacaaccga gcagtgggct gcggatgtaa
    10861 aagctgacaa agaaatcggt ggcgacaagt tgatttctaa ccttagcgcc gcacagcgtg
    10921 cgcttgacca gttcgggaca cctgagctca aagaatatct gaacaccacc gggctgggta
    10981 atcaccctga tctggtcaaa acgttcgtga aaatcggaaa ggcgatgtct gaagatggca
    11041 tggtcaccgg tggtaatgaa ggccagcgta gtgcggccga agtgctctat ggcaaataag
    11101 agaggaaatg acaatggctg ttaaaggctt aactgcgcta acgctggctg actggggtaa
    11161 gcgcgtcgat ccaaacggga aagtcgataa gattatcgag cttctcggtc aaactaaccc
    11221 gatccttcag gatatgcctt ttgtcgaagg gaaccttcct accggacacc gaaccaccat
    11281 tcgttctggt ttaccttcag ccacctggcg tttgctgaac tatggcgtac agccaagcaa
    11341 atcaaccaca gtgcaggtca ccgattccgt tggaatgctg gaaacctatg ctgaagtcga
    11401 taagtcactg gctgatctga acggtaatac cgccgaattc cgcctgtctg aagaccgcgc
    11461 atttattgaa gcgatgaatc agcagatggc gcagacgttg ttttatggtg attccagcgt
    11521 taaccctcag cagtttatgg gactgtcctc ccgctattcc agcctgtctg cgggtaatgc
    11581 tcagaacatc attgatgctg gtggcacggg tacagataac acctcaatct ggttagtggt
    11641 gtggggcgaa aacaccgtgc atggcatctt cccgaaaggg cagaaggctg gcatccagat
    11701 ggaagataaa ggccaggtga cactggaaga tgttaatggc ggcaagtacg aaggctaccg
    11761 tacccattac aaatgggaca acggacttgc tctgcgtgac tggcgttatg ttgttcgcat
    11821 tgcaaacatc gatgtcagca atctttcaga accttcctct gccgcaaata ttgcgaagtt
    11881 gatggttaaa gcactgcatc gcattccaaa ccgtggcatg ggtcgcccgg tgttctacat
    11941 gaaccgcact gtaggccagg ctcttgatct gcaatctctg gagaaaacat ctctggctat
    12001 cagcgtaaaa gagactgaag gcgagtggtg gacttcattc cgtggtgtac caatccgtga
    12061 aactgatgcg cttctggaaa cagaagcccg cgtggtgtaa cgcctgttat taacctgtgg
    12121 gtcgtaacag acccactaat ggagaaagaa gatgatcacc gacaaactgt tgatgttctc
    12181 cgaagctcag gcggttacga ataccgcggc ttctactgac gtaatcgatc tcggtccaat
    12241 tgacggaaaa cgtcgtgata tcggcgtagg ttacccgctt gagttttggg cgctggttaa
    12301 cacagccgcc gcggcaagcg gtgatgcaac tgtaaacatc cagttgcaga cgagtgagaa
    12361 taacagctca tggaccacta tttatgatag tggcgcactg gcaaagaccg ccctgacagc
    12421 aggtaaacga gttgtttctg caaaggtgcc tgccggtgtt cagcgatatc tgcgtgttaa
    12481 ctactccgtc gcaactggcc cactaacggc tggcgaattc actgcgggta tcagtcttga
    12541 tgttgatgcc aatacgccgt atccgatccg ctcaaaagta actggttaag gtgatattga
    12601 tgtcaggtga gaaaccaaga taccgcgttc tgcgcctctc tcatatccat aacactctgt
    12661 ggccggaggg ggcagaaatc gaatacgaag gtgagcctgg tagcgcactg gaacctgtta
    12721 acgatgcagc cagacaggca aaagcaaaag ttgcaggaaa ggtgtcaatg gcagcaacca
    12781 gcaccaaaat catcaacgat gtgtcagatg atggtgaact ggataagctc cgtgaagagt
    12841 acgaattgct ctttaacgag aagccacacc ataacgccaa agccgaaacg ctccgcgaga
    12901 agatcgcaga taagcgtaaa gaactgggcg tgtaagcctc gcgaatcaga caaggggctt
    12961 cggccccttt attgcaggag tgtatatgga actcgtaaac ctcaaaaccg gcactgacag
    13021 ctaccaggat gagagcggag aaaccagaac tcgcgatgaa tacccgtggg ggctgtgcat
    13081 cacgctgaat aacgacacat tgaataagct gaaggcgcaa cctcagggcg tcggaacaga
    13141 agtgatgata actgcaaagg ctgttattcg aggcctgtct gccagagaaa ctgacgatgg
    13201 tgttaatcgc agcgccgatc tgcagatcac tgatatggca atcgctcctg tttccggtga
    13261 tgtagaaaaa tcagcggctg aaactctgta cggcaatggg ggtgagtaat ggcctctgta
    13321 gtagagatct gcaatcgtgc gctgtccaat attggcaaca gccgcagcat taacagcctg
    13381 acggaagcca gcaaggaagc gggggaatgt tcgctgcact ttgaggcctg ccgtgatgct
    13441 gtgctttctg attttgactg gaactttgct accaaacgcg tggcgcttgc agatacgagc
    13501 aatccaccgc ctgactggga atatgcgtac cagtacccgt ccgattgtct gcgcattact
    13561 gaaattatgc ttcctggtgt acgcaatcca acagcagcaa tgcgcgttca gtacgaagtt
    13621 ggtgcagaca ccaacggaac aggaaaattg atctacacag accagccgca ggcatggctc
    13681 aagtatgtct ctcgcgtttc agatgtgaac atgtttgatg ccatttttat ggaggcgttg
    13741 gcctggcgtc ttgcggcagc tattaacatg gcgctgactg ggaatgcaga cctcggtacg
    13801 tttgccctca atatgtacaa tcgcgagatt cttagtgctg gctcgcatag ccagaatgaa
    13861 tcacaggaac ctcagccacc ggttgatgag tttaccattg cgaggttgtc ctgatggcta
    13921 tcagttggat ccagcccagc tttgctggtg gtgagattgg accgtcgttg tacggacgta
    13981 tcgacatggc gaagtaccag gtggcattgc gcaagtgcga taactttatc gtgcggcagt
    14041 atggcggcgt tgagaatcga cctggtacgc gttttgtcgg tgccgccaaa tacccaaatc
    14101 ggaaatgccg cctgatcccg ttccagttct cgacggttca gacttatgct ctggagttcg
    14161 gacaccagta catgcgcgtt atcaaagatg gtgcgttggt gctgaacagc agcaatgtta
    14221 tttatgaaat tgccacgcca tatactgaag ccgatctgtt cagaattaaa ttcacgcaaa
    14281 gcgcagacgt gcttacgctg gttcatccgg catacccgcc gaaagagttg cgccgctatg
    14341 cgcatgacaa ctggcaactg gttgatgtgg taacgaagaa cgggccattt gaagatatca
    14401 atattgacga gtcagtgacg gtttatgcca gcgccagcac cgggacaatt acgttaacgg
    14461 caagcgcctc tatttttggc gtggagcagg taggcaaatt gttctatctg gaacagccag
    14521 cagtggattc agtaccggta tgggaaacca gtaagagtac gtcgattggc gatattcgcc
    14581 gtgcagacag taactactat cgcgccgtta cagcaggcaa aacatgcact ttgcgccctt
    14641 cgcatacaga aggcacatca tgggatggct ggggcggatc cggtgatgat gataccggca
    14701 ttgagtggga atatctgcac agtggttttg gcattgcccg tatcactgct gcaaatggaa
    14761 ctactgcaac tgccgaggtg atttcctata tcccttcgca ggtagttggt gcgaataatg
    14821 caagctataa atgggctaaa tatgcctgga acagtgttaa cggttatcct ggcactgttg
    14881 tttattatca acaacgtctt tactttgccg catcgactgc gttccctcag actatctggg
    14941 ccagccgtac cggtgattat aaggattttg gtaaaagcaa tcctacgcag gatgacgaca
    15001 gaattatcta cacctatgcc gggcgtcagg ttaatgagat ccgtcacctg attgatgtcg
    15061 gttcgctggt ggcactgact tccggaggtg agtacgtcat caccggcgac cagaacaaag
    15121 tgttaacccc atcatcattt gcattcagct ctcagggatc aaatggctcg agcaatgtcc
    15181 caccaattgc cgtggcgaat attgctctgt tcgtccagga gaaaggcagt gttgtccgtg
    15241 atctggccta ctcattcgat gttgacggct atcaggggaa cgaccttact atccttgcca
    15301 atcatctttt tcagaagcac agcattgttg actggtgctt ctcgattgtc ccttactcca
    15361 gtgccttctg cattcgtgat gacggtaaat tactggtgat gacctatttg cgtgatcagc
    15421 aggtttttgc atgggcacca caatccagta ccggaaaata tgaaagcaca tgcagtatca
    15481 gcgaaggcaa tgaagatgcg gtgtatttcg tcgttaaccg aaccgttaac gggcaaacag
    15541 tgagatacat cgagcgactg tccagccgtt tatttaccag cgatgaagat gctttctttg
    15601 ttgattctgg ccttagctat gatggaagaa atacgtctga cagaacaatg accatcactg
    15661 gtggttctgg tgaatgggat taccgtacgg aatatacaat cagtgtttct ggtggtgcgt
    15721 acttcaccag tagtgatgtc gacgcgcaac tacagttccc ttataccgga actgatcctg
    15781 atactggcga tgaagtgtca aaagaattac gttgcgacat tatttctgta accagcaata
    15841 ccgctgtagt ggttcgtgcc aacaggaacg tcccgccatc cctcaggaat gcggccacca
    15901 cgaactggca gatggcgcgc cggacatttg gaggcctgtc tcatcttgaa ggccagaccg
    15961 taaacattct ctctgatgcg aacgtggaac cacagaaagt ggtttccgga ggtgccgtca
    16021 cgctggaatc accgggggct gtagtgcaca tcggcctgcc aataacttct gaattcgaaa
    16081 cactggatat caacattaac ggacaggaaa cactgctgga caaaaaacag gtgatcccgt
    16141 ccgttaccct ggtggtgaat gccagtcgcg gcatctgggc gactacgccc ggcggtaaat
    16201 ggtacgaata tccacagcgt gaattcgagt tctacgatga tcctgttgat gacgctaccg
    16261 gaaaagtaga agtgaaactg gacagtaact ggggaaaaaa cggacgtgta agaatccgtc
    16321 agcttgaccc gttgccgctg tctgttcttg ccgttattcc tcgccttact gttggggggt
    16381 tctgatgatc gatgttcgaa ttattcccgc taccgaagag catcttcaga tgattttgcc
    16441 ggatgttcgt caggctgata ttgacgaact gtatgcggta tcgctgatga ctaccgaaga
    16501 tgcgctacgt gttggtctgc gcactgcgac tatggcctgg tcagggtttg cgaacagaga
    16561 actggtaacc atgtttggtg tatcaccggc gtcaatgatt ggtggcaatg gtacgccctg
    16621 gctggtcgga accagccgta ttgaaaaata tcagaagaca tttctgcgcc actgccgccc
    16681 tgtattgcag cagatgctgg cagtttatcc gcgcctggaa aactacgtcg acgagcgaaa
    16741 ccatgttgcc aaagcatggc tgcactggct tggattcagg cttgaagaag ccgcgcctta
    16801 tggtgctctt ggtcttaatt tccacagatt tcacatggag agaaaataat gtgtgatccg
    16861 gttattgctg gtggcgcaat gcttgccatg agtggcattc aggcatacac ccagtaccaa
    16921 cagggaaagt atgcctcgaa ggttgcagaa gcgaacgcag atatagccac tgctcaggca
    16981 aatgatgcaa taaacagagg taacgctgaa gctgagcaac ggcgcagaga gacccggcag
    17041 cggcttggca cacaagcggc gacaatgggg gctaccggcg ctgatttatc taccggtaac
    17101 gcgctggata tatttggtga cactgctcag tttggcgctc ttgatgcgct gacgacggtg
    17161 aataacgcgc aacgtgaggc ttacggttat caggttcagg ctgccaacta taaagcagaa
    17221 gccagttcag cccgtaaaca ggggaatgtg ggagcagcaa caacattgct cactgcgcct
    17281 ctgaaggcat acggtgcgta ccagatgttt ggtgggacgt ggagtccgtt tactcaaagc
    17341 actcatgcgc caatcggggc agcagcagga accagattac ccggaggatt ataatgccag
    17401 tcgtaccaac agtatccgga cgccaggttg agagccgtgg agttcagtca gcaggcttgc
    17461 agacgttttc tcagccaggt attggtgatg cttttgttcg ggcagggaca gaggcaattg
    17521 atgttttggg gcaggcaaaa cagcgtgcca atatcgctct ggctcaggag gcatctctta
    17581 acctcagtca gataagcagt gatctgctga ataaccctga aacaggtttg cttaacctga
    17641 aagggaaaaa tgctactgga aaaggtcagg agtatacgca gcagtttgat gcccaggtcg
    17701 agcaactggc tatgtcgctg ccggatgaac aagctcgtaa tgctttcatg cagcaggcgc
    17761 agcagcagcg cattcagttc actacgcagg ccgggcggca cgagataggg caaataaatg
    17821 cctacgaaga aggccagttt caggcgacgc tgctgaacaa tggtaaaaat gccgcagcat
    17881 tgtatggcga caacgccgca tacgtattgg ctaataagca aactttccag caaattgagg
    17941 attacggcat tgcgcatggc tggagcgacg agcaaatcca ggccaagaaa atcgagttta
    18001 aagagaaggt tgctgatgcc gcattgtccc agtggtcggc aagcaatgcg accgcattca
    18061 tccaaagtaa tggcgagtta agtgatactg ctgctggagc tcgccgtgct gtagcagata
    18121 atgactcttc cgagcgtgcc cgtggcatac gcaacaataa cccaggaaat ctcgaataca
    18181 gcaaaactaa tccgtgggta ggccagaccg gtgatgatgg tcgatttgct aaattcgaaa
    18241 cacctgagca cgggattcgt gcattagggc gtaacctgat gtcgtatcag cggcagggta
    18301 tcgataccgt cagcgagata attaatcgct gggcaccgcc tactgataaa aatgacacta
    18361 tgtcgtatat caaagcagtg tgcgaacaac ttggcgtttc tgctgatgag cctctcgatg
    18421 catcaaatcc tgataccctg aaggcgcttt gtgcagccat tatccatcat gagaacggta
    18481 gtcagccata cagtgatcag cagttaactg ctggtgtcag tgcagcactt ggtttatcaa
    18541 caattccaac caacaccaaa cgctataccg gtaatgcagc attcgatgcg gcatctcctg
    18601 aggctcaggc aagttttatg cggcaggcgg atcaactgcg tcggcagcag caggctgaat
    18661 ataaaacgat gattgacagc caggttcgtg atgcgacagc tgcgtatatg cgtggcgttg
    18721 aatttcctaa cccacctggt gaggatgatt ttattgcagc ttatggtgtc agagaaggaa
    18781 acctgcgata taccgagttt aagaatacgc agatcgccgg acagtatata ggttctttcc
    18841 gcaacatgcc gacaagcagc attacagcat atgttgagca attacgcccg gatactggtg
    18901 agacagggga ggggtatgcg gcacgcgcag ctctttatga caacgttgtt tcggctgcaa
    18961 atcaggtgat aaagcagcgg caatcggatc ctgtgcagtt ctctcttgcc gccggacagg
    19021 caaagcctat cgacatgagc aataaggata actttggaca gagcgttgcc ttgcgtgccg
    19081 ctcaggtcag tgaccttgcc aagtcatatg gcactccact gacgttcttt tccaaagacg
    19141 aggccaatca gatcggtgtt ttctttcgtg atgcgcccgt ttcccaacag gcagcatatc
    19201 tcgataccat caggcaaagc actggcggtg ggcaggtgta tatgtcagca ctacagcaga
    19261 tcagtgccaa cgctccatct gctgccgttg ccgggatact gatggataag cctggtggta
    19321 ttttggcaga aaaaaactgg tttaatccgg atgtttccgt gtctcctgaa accgctgcgc
    19381 agacaattct tgctggcgcg gcggctcgta aaggtactga tgatgcgaaa ggtattccga
    19441 tgcctaaaga tgctgatctt cgccttgagt tttctgacat ggtgaaggat gcatttgctg
    19501 gtgatgctca gggggcatca atggcatacg agatcgcaaa ggactattac gctggtgtga
    19561 tggcgaaaaa aggcgtggta tcaggcgaaa ttgacaatga tatctggaaa caggctgtta
    19621 acgtagctac aggtggcgtg catgactata acggaatggg gaatgttctt ttgccgtggg
    19681 gaatgtctgc agagcaattc gataagcagg ttaatcaggc ttggaatgaa caagttgtcg
    19741 gcacagggat aaaaacaccg cctggtcagt atggtttgca aagttacggc gatagtcagt
    19801 acctggtaaa acttgggact ggatatctgc tgaaggatga tggttctcct gttgttcttg
    19861 atctgacaca gaagcgtcag agattctccg gagatattcc gcaatgagtt actttggcct
    19921 taatccagta aaccagaatc agcagcttga cgaagcagca tcaaatccag ctggctttaa
    19981 cagcgatgtt ggttttttcg acaatgctgt aggagcggca ttgtctggtt tgtactccgg
    20041 gctggtggca aagccagatc agttgctatg ggcagggatg gataaaatcg tatccccgat
    20101 tgctcagttt gttaacgaaa acacctcgct caatgacact tcagtttcat acattgccga
    20161 gcagagaaaa ctagcagagc agcaggttaa gcggctgacg cctgatgccg cgacaaccgg
    20221 aaccgcaggg caggttcttt atgggttgtt cgatatgggc gggcaggctg ttgtcggtac
    20281 aacgctcggt ggtccggtcg gaggtgctgc ggcggtaact tcgctacagg gtttttctga
    20341 gtttgaacga ctgacagctc agggggttga tttcaggacg gcgcaggaag cgggattagt
    20401 gcagggtatt actgctggtg ccggaacact gatccctatg agtctcgggt tacgtgctgg
    20461 tggtgcgcta gcggaaggtg tggcggctca gcttgcgcgg acgggtgaaa gttcagtgcg
    20521 acgcgccgcg gcaacagcag tacgtgcaac gccagatatt gcctatgccg caggtacaaa
    20581 tattgcgttc ggtatggcac agcgcgggct tactgcaaaa acgcttcgtg atggtggcta
    20641 tagcgaaatg gctaaccagt atgatgtgtt ggatcgacag gcaattgcta ttgatgctgt
    20701 tcttggggtg gcttttggtg gagtcggcag atttattaac tctcgcggcg agtctacaaa
    20761 cgcaccaaac ttttcaccag ttgatatcga tgctgcactg gcggcgaatg ccgctcatca
    20821 tgctgaaatt gatattgctc ccggcgtgcc gatcaacgtg ctttcgcgca attctcacat
    20881 tcaggctctg cgaaaagcta tgtctgctgt tagccagggg agacctgtag acgttgccag
    20941 cattgttgag tctgcatctt tcagtgaaat tcctgggcgc aagaatctgc tgtctcaggc
    21001 agttaatgag gctctgtcat ctgtagatga tggagtaacg gcgcgcgcta tcgaaaatcg
    21061 gttgcttgaa gaacaggctg cgcagctttt gccgcgtggc gatagacagg tttaccagtc
    21121 tgaaatcgct aatagccaac gaattctcga caatctcact gaacagcgcg cacaaattct
    21181 tgcagaagag ccaactggta gcggtaaagc tttgtctcgt gctcgatcag ataaacaggc
    21241 cagaattcgc gatattgacc aacgaatccg gcaggcacaa gaacgcctgg aattttcccg
    21301 taacgcgttg gcgccgcacg agcatggagg tcagtttttt gaagctcgag cagaactggc
    21361 acggcgactg caggtagaaa gtgaacttaa tgctcaggct gtttcattct ataaaacagc
    21421 agaggtcagg acgccagacg aagtagctcc ttttgagcct gataaaatat tgcaacaggc
    21481 agaacaaaaa atgatggcgg atcaggcagg agatattgat ctgcgcatag ctgaagactc
    21541 gttgcttgaa tcacctgaca tgataatcac cgtgctggat gatgatggta atccacaatc
    21601 gcgcagcgcg cgtgaagtac tggatgaagc gaacagggaa agtgagcagg caatacagga
    21661 ttccagcctg tttgatgtcg ctgtggcgtg tttcttgaga ggttaaatta aatgagacag
    21721 gaatgtatac aagcggtcca gcaggcggcg cagcgcacgt taacggcgcg agaaatacag
    21781 aacattgaag accgcattta tcgaaatatg cgctccattg ctcgtgatga cccgatgtcg
    21841 tggagacaac tttccgaatc agagcggcta tatcgtgcag cacaattggc atctgaagaa
    21901 ttacagcgag aagcggcatt aaagaaacgt cgtgtggccc tcactatagc cgcacgtcag
    21961 agattggata aatttatcaa tagctatcaa ggggctgatg ggaaactcgg cgctcttaac
    22021 cgtactattg cttttaatgc agacggtaaa tcgaattttc tctctgttga gtccagaaca
    22081 aaagccactc gtgattatgc attgagtcaa ttgcaagagg catttgaagc agttgatcct
    22141 cgcttttttg gcctgtttga agatgaagct ggcgtgcgtg acctggtata tgaaatgcga
    22201 gggcaaaata ctggcaatgc taaagcaaga aaaggtgcta aggcgtggag agaagtgaca
    22261 gatctactgc gccgccggtt taatgatgct ggtggggaca ttggctatct cgaaaactgg
    22321 ggaatccctc aacatcattc tatggaaaag gttggggcgg tatcaaagga taagtgggtt
    22381 agcgatgtta taggtaagct ggatcgcaaa tattatatcc gagccgatgg acaactgatg
    22441 aacgatgccg agttgtctgc atttcttgga gaggcttata acacgatcgc tactggtggg
    22501 ctgaataagc ttactgatac cggaatgcga atttccggcg cacgtgctaa ccgtggtaat
    22561 gcatcacgac agatacattt caaagatgca gattcctatc tgcaatatca gcaactttat
    22621 ggcgatcgct ctctatggga aatcatggtc ggtcacctgg aaggtatcag taaagatatt
    22681 gcactggtgg aaacatatgg cccaaaccct gatcatgttt tccgctctct tcttgatcag
    22741 gtgaaggcag aaacggcaac agctaacccg agtaaaaccg gtagcgtcga gcggctggcg
    22801 aacaaaacag agaacctgta caactttatt tccggaaaga cacagcctgt tgcgaatcct
    22861 cacatcgcgc gctggtctga caatatcaga aactggctgg ttgccagcag gctcggatcc
    22921 gcgttgctgt catcgttctc tgatcttgga accatatatc tgtctgcgaa ggttaccaac
    22981 cttccaatga accagttatt ccgcaaccag cttgaagcta tggacccaac gaaccgtact
    23041 gagcttgcgc gggcgcgccg cgctggtctg gcgatggaat ctctacttgg cagcgttaac
    23101 cgctgggcga tggataatat ggggccgtct gtgtctcgtt gggcggcaac ggcggtaatg
    23161 cgtgccagtg gacttacagc atggtcagat gcgcacaagc gcgcttatgg cgtaaccatg
    23221 atgggaagcc tgggagaagt agtgtcacgg acaccagacc ttcgtagcct cgatgactct
    23281 gattttcgta tcctgaaaag caaagggatt actgacacag actggagcgt atggaagctg
    23341 gcgcaacagg aggactgggg gaacggtaat aatacgatgc tgacaccgga aagcattatg
    23401 cgtatccctg attcagcagt taaacatctt ggtgagcctg aacgtgtgaa atttgaggca
    23461 atgcgtaaac tgctcggtgc cgtaactgaa gaagttgata tggctgttat tacaccgggc
    23521 gcacgtgaac agatgttcgt agggtctggt cttcagcgtg gaacatggaa aggtgaatta
    23581 acgagaagtg ttttcctgtt taaatcgttc cctatctcgg tagttatgcg tcactggcat
    23641 agagctatgg ggatgccgtc tgctggtggg cgtgcggcat atatagcaac gtttttagca
    23701 agcacaacca tgcttggtgc tctttccatg cagattactg atcttattaa tgggagaaat
    23761 ccaaaagaaa tgaccggtga caacatggtt aaattctgga taaatgcatt tttaaaaggt
    23821 ggtggggcag ggttgtatgg tgattttctt ttctctgacc acaccaggta cgggagcggc
    23881 gcactagcgt cgatgcttgg cccggtagct ggtctggttg atgacgtagt gaagattgct
    23941 cagggtatac cgttaaatgc tgtggaaggg aagaatgagc agactggtgg tgatttggtt
    24001 aagcttggga aaggtttgat gcctggtgcg aatctctggt atttgaaggc ggctctcgat
    24061 cacatgatct ttaaccagat gcaggagtat ttttcaccag gctatttgcg taaaatggag
    24121 caacgttcaa agaaagagtt taaccagaca tactggtggc gacctcagga tgtcactccg
    24181 caataaggaa caacaatgaa ggaattattg ctgttatcgg tatttttgat atccgcttgc
    24241 tcgacatcct atgatgttta tgacggtgtt gataaggcat attgcgacaa agttaaaatg
    24301 gatttttctc ttgccaagac ggcgaaggat agttgtattg atcactacgt caagacttat
    24361 accaagccag cctcatcggc atctgatatt gctgaaggtg ctgtgtttga gtgtaacaag
    24421 gtgatatcca tcgcagcgag ttcttcatac gatgctgctg tatgtgcaat ggctgaaaga
    24481 aacggcatgt cagtgcaaaa aattaatagc atgataagca gtaatgacga agccaaaata
    24541 agaactgata tcagctcggt gaaaaaagat gccatgaaca gagttgtaaa atatcagtca
    24601 tctttataag tcgtgacatg tcacaggccg ctttcgcggc cttgttttta acgaatgcca
    24661 ccgccgcccg ggcgggaatc cgcagaacgc ccaccgcagc gggagccgtc agcggcagta
    24721 tcgctgtcgt gctgacaacg accggcaaag gcctgagttg aagctaccag agacaacaaa
    24781 acgaacagtg cagcaaatgc ttttttcatt gtgaaatttc catctataag ccacctcgat
    24841 gtggcgtcaa tgagtgtagc actgactttt gtttcgtcca caaaaaagcc cgcggcgcgg
    24901 gctattcctt ccatttatca gaaaaaagat cttcttctaa aggcattggt tctgtttttg
    24961 ttttctcaaa gaattgataa ctgatagtga ttgctgcctc tttaaactct tcttgctcag
    25021 ttatgttgtg agcatctgcg tcaacaaaaa acataaccag cgcatcacga ttgtgattta
    25081 ccgaataaac taaaaagcaa tcacttgttg gtatgcattt tacttgtaca gacgctatat
    25141 ttttccatgc atcccaagat gattttttac cagtgtgttt tttatcacta tcttctggaa
    25201 tatagtcttg gttatcgaca tgagtatgcc tgacatttag tttaagcatt tctgtcggac
    25261 gagcaaatgc agcatcttta ccaagagatg gatggtatcc cgttttccaa tactgggtaa
    25321 aggcatcaga tactttcttc agctcaagat cagatgcaca aagggccgag aaattttgcg
    25381 tatgcaatac tcgaccctta tatctgacaa tttgattttt atcattctgc gacgcagacg
    25441 aactcataat tttccttatt gttacgagaa tcaaagaaag cgcgggatac gcgacttgcg
    25501 tgatcttttg tcatagtaac ttttacgtac tctacgcttc cattgaaaga acgtcttgcg
    25561 gcagcttgcg ctctacgcat ctgcaatttt tcgtttcgca tgacattacc tcatatctca
    25621 taagttcatt acacggatta ataaaaatga aaccaatccg tttacccttg aggtaatagt
    25681 acgctattca cccacagtct gcaatctgta cagaattatt taaaagcaca tccatgtgcc
    25741 accgcccgtc agaagaatcc tgccttgtcg ttgatgtatt ccgcgtgggt ctggatatca
    25801 cgcaggcatt tactcacacc gacgatgtag cagaacatgg tggtcagttc cgccgccgcg
    25861 cccgatacgt catgcccgtc ttcctgtaac tggttcagca gattcatcag cagtgagttc
    25921 tccgtcaggc cgagaacacc agacggcgag tgaatcaggc tgcggtagcc gggcttcagt
    25981 ggggcactgt aggttttgtt ctctatcttc attgcctgta ttactgctga cgccgtggcg
    26041 ttggctacct ggtcggcaac catctttatg cgttcttcct gcgggagcga gtttttaatg
    26101 taacttccgg tgcggcggat ctgaggaaga acctcacctg taacccattt acgaaagcgg
    26161 taggggatag tgcctggtgt caccgcatcg cggcagcgga ggatcagtgt gtagaggcct
    26221 gactcggaga tgatgttgat ctcttttact cggctgtcaa aaattgcacg atgttcatgc
    26281 cctatgttga acatagacct ttcatcatca tccagttttt caagtgattg ggtgacgttt
    26341 tggatacgca gcgcactaca aacgtcttgg gctacaaacc atggttggcc atcgataatg
    26401 atggaacgga tagggttaac agattcaaat ttgaagatgg cagtttgagc attagccatg
    26461 gtggttatct ccacttagtg attttaatca ccaccgcaac gccaattact ggtggtgaac
    26521 tggacaaggt tggcgtaccg gcctaagtgg taccggcgtc ctttcggacc cctgcccagc
    26581 ccaccataat tcggatatgg ctgtgcttaa cgcataaaaa aaccacgtct ggcgtggtat
    26641 gcgccactta gtaactcggg acgccaatcc cggcactgga ttttgccagt acccgattac
    26701 tatggcacaa gaggagtgcg atgtaaattt acctcaaagg taaatataag cactccactt
    26761 ggtaattgca aaccttatct ggtttgtttt cgtaattgtt cggcacaata gtcgagatgt
    26821 gtttgcagat cctgcataga catctgtgag ctggtgacgt agttaatcag tgcagtcagt
    26881 tcggcaagtg ggccatcgac attaaatcca tccttatcga gatcccggag taatttcatc
    26941 aagagcgatc cctccaccag taatctgacg cctcccggcg tgtgaatcct ttcggtaaat
    27001 ccgtcttcca gtggatagtg atactgctgc atcttatctt ctccatgcaa taactgtaca
    27061 aatatacagt atcaaataat ttgtttgcta tccagcacgt tttgcaaatc acctgaaagg
    27121 taatatctgt gtgtattgat gatgtttcta tccatatatg gttcgttggg taataaaata
    27181 gccagatatg cggcgcaacg ggtgctgcga ctatctggag atttagcatg acggtctcaa
    27241 ccgaagttga ccacaacgaa tacacaggta acggtgttac gacatcattt ccgtatacct
    27301 tcaggatttt ccagaaatct gatctggtag tacaagtggt tgaccttgat gaaaatatca
    27361 ctgttcttgc tcttgatact gattacacag ttaccggggc gggaggatat aacggtggta
    27421 atgtaattct gtcgaaggtg ttggctaacg gttatctgat ttctatatat cgagagctac
    27481 cggttacgca ggaaactgat ttgcgtaatc agggtaagtt ctttgcagaa gtgcatgaga
    27541 acgcttttga taagctaacg atgctgatcc agcaggttcg aaattggttc agtctggcgc
    27601 tacgaaagcc gtcgttcgtg gctaattatt acgacgcgat gaataactat attcgtaatc
    27661 ttcgtgaccc atctaggccg caagatgcag caacgaaaaa ttatgttgac gggcttgcag
    27721 aaacaaacct gagcagaacg ctacgaactc ccgaaccgat atcaactttg cccggcgttg
    27781 agcaacgtaa gaataagatt gttgcaatgg atgattcagg agacccaatc atggttctcc
    27841 cggaatctgg gagcgctact gatgtcatgt tgcagcttgc tgccaccgat gggtttaaaa
    27901 atatcggttc ctgccctgat gtggccacat taagaaatac tgaaccttcg tcagtaaacc
    27961 agcaaattat tgtcagaggt tattatagcg atacccccgg tcttggaggt ggggtgttca
    28021 tgtcgtttga cagttctgaa gctgacgatg gtgtgaatat ttttgttacc cccggcggta
    28081 agcgatggaa gagagctgga agccacatcg atattccggt agagaacggt ggcatgatga
    28141 catcatgtac ggctgagcaa aacagtgaag cctttgaacg cctgactgca tgccttccat
    28201 atgaaggagg tacgctcaga ctcaatggat tttacgatat taagtatggc gctatcgtgc
    28261 ctccacgtgt cacgctggag gggtgcggta tggattcctg tggattaatt aaaacaggta
    28321 acgatattaa aaccgttccg gatcgcatgt ggcagggtgt accacacagc ttctccaaag
    28381 actttattgt tgcggtagat atggatagtg acacatcagg tgatttaagc gggaaacaaa
    28441 caagaagcac cagaataatt ggattgagta ttctaaatac tgcgcccaat ccttgtgatt
    28501 acgggatata tagcgctatc agttacaatg tgcgccttca ggacctgtat attcataggg
    28561 tgaaaaccgg ttatcgcact agtgatagct ggttgcagac atgggaaaac attactattc
    28621 aggatgtcgt tactggagtt aaaatcgaag caggtggaac cacatataat ttaaacaata
    28681 tttatgtgaa aacatgttca ggtgttgcat atgattttaa taacattaca tatagcagcc
    28741 tgacttgttt ggctgctgac ttcgttaatg gtactggcta taaatttaaa gattgtactg
    28801 gagtaaatct atctggatgc ggaagcgaag aaggcactgg taaaatattt gatataaatg
    28861 gtagtcgaat taacgtaact gcatttagag gggttaatca tcatgatgga ggagttgaag
    28921 ctgtatctct ggaatcgtgt ttcatctcat tggtttcatc tcatttttct ggttactctg
    28981 gagtctcatc tagcaaatac atatatctta atgatgcaac tgcaaatttt aatggtgcgg
    29041 taattcccga caacaccact gattctatta gatgggggag tgggcagagc tggatcaaca
    29101 aggttgattc aggcggcacc tatagtataa ccggaaactc atcatggact gcattatgca
    29161 gacttgagtc aggatatata acggttctat cagtatcacc acccacggga gatacaacta
    29221 atttcccgtt tggcaccaag tgggaattgc ttggtccggt ttctaccgga ccgtggaagt
    29281 ggacatacgt atcaactggt gaatggaagg tatccgcaac taccacgtaa aaaaaggggc
    29341 tgaaagcccc tttttatcta atatctgaat ttgtttgata tctttttccc aatatccatt
    29401 gctggttttt ctatcgcaat aaatgatatc agtccagcaa taagtgatag tgctatgcat
    29461 aaaacttgat ttaagtacag atttatatca ggtaataacc tgaatgttat ctgctgtatt
    29521 aaaaagcccc acaagtaaac gccatatgat acatcatgtt ttattcttat tgattttatt
    29581 atttttgttg ttgataaata tacgaaaaat gtacatgcgc aaaaataaaa aagcatatgc
    29641 ttgagtgtt
//
LOCUS       DAZGJT010000054        29363 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZGJT010000054 DAZGJT010000000
VERSION     DAZGJT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.054"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(81..1355)
                     /gene="fadK"
                     /locus_tag="ACHKFK_002654"
                     /pseudo
     CDS             complement(81..1355)
                     /gene="fadK"
                     /locus_tag="ACHKFK_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(1412..1705)
                     /gene="ydiT"
                     /locus_tag="ACHKFK_002655"
     CDS             complement(1412..1705)
                     /gene="ydiT"
                     /locus_tag="ACHKFK_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO9052957.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIEFRYG"
     gene            complement(1702..2991)
                     /gene="ydiS"
                     /locus_tag="ACHKFK_002656"
     CDS             complement(1702..2991)
                     /gene="ydiS"
                     /locus_tag="ACHKFK_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9052958.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPIERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGTQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVSASDPHHYAVGVKEVIGLTPEQINDRFNVTGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLINGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVHGMDLAIASAQAAATTVIAA
                     KERADFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNDMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(3046..3984)
                     /gene="ydiR"
                     /locus_tag="ACHKFK_002657"
     CDS             complement(3046..3984)
                     /gene="ydiR"
                     /locus_tag="ACHKFK_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO9052959.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTEQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDISH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLEISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(4004..4768)
                     /gene="ydiQ"
                     /locus_tag="ACHKFK_002658"
     CDS             complement(4004..4768)
                     /gene="ydiQ"
                     /locus_tag="ACHKFK_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO9052960.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYMVQDAQLEHALPLNTAKALA
                     TAVEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIGWSQSAPLAEL
                     TGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            5084..5995
                     /gene="ydiP"
                     /locus_tag="ACHKFK_002659"
     CDS             5084..5995
                     /gene="ydiP"
                     /locus_tag="ACHKFK_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9052961.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLAG"
     gene            complement(6038..7189)
                     /gene="ydiO"
                     /locus_tag="ACHKFK_002660"
     CDS             complement(6038..7189)
                     /gene="ydiO"
                     /locus_tag="ACHKFK_002660"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO9052962.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAAPYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(7203..8798)
                     /gene="ydiF"
                     /locus_tag="ACHKFK_002661"
     CDS             complement(7203..8798)
                     /gene="ydiF"
                     /locus_tag="ACHKFK_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO9052963.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SDLAEQNKIIAYNYPQGVLTQTLRAAAAHQPCIISDIGIGTFVDPRQQGGKLNEVTKE
                     ELIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQTQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIVFCGTLTAGSLKTEITDGKLNIVQEGRVKKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(8944..9702)
                     /gene="aroD"
                     /locus_tag="ACHKFK_002662"
     CDS             complement(8944..9702)
                     /gene="aroD"
                     /locus_tag="ACHKFK_002662"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO9052964.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHYADLSNVESVIAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(9733..10599)
                     /gene="ydiB"
                     /locus_tag="ACHKFK_002663"
     CDS             complement(9733..10599)
                     /gene="ydiB"
                     /locus_tag="ACHKFK_002663"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO9052965.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(10611..11876)
                     /gene="ydiN"
                     /locus_tag="ACHKFK_002664"
     CDS             complement(10611..11876)
                     /gene="ydiN"
                     /locus_tag="ACHKFK_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052966.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYCLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGEHKFSTRLNTIKHRG"
     gene            complement(12103..13317)
                     /gene="ydiM"
                     /locus_tag="ACHKFK_002665"
     CDS             complement(12103..13317)
                     /gene="ydiM"
                     /locus_tag="ACHKFK_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9052967.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRHLPVIKKTTSSTEHRCSIIDLASYSLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALLTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFILALFIGLRSRKETR
                     HHTLKENIAPGG"
     gene            complement(13417..13773)
                     /gene="ydiL"
                     /locus_tag="ACHKFK_002666"
     CDS             complement(13417..13773)
                     /gene="ydiL"
                     /locus_tag="ACHKFK_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO9052968.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGNSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(13909..14016)
                     /gene="rprA"
                     /locus_tag="ACHKFK_002667"
     ncRNA           complement(13909..14016)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKFK_002667"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(14202..15314)
                     /gene="ydiK"
                     /locus_tag="ACHKFK_002668"
     CDS             complement(14202..15314)
                     /gene="ydiK"
                     /locus_tag="ACHKFK_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO9052969.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKSNK"
     gene            15528..18584
                     /gene="ydiJ"
                     /locus_tag="ACHKFK_002669"
     CDS             15528..18584
                     /gene="ydiJ"
                     /locus_tag="ACHKFK_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO9052970.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDETLIDERVNALCARLDELIASQQAGVIGWQVCSEL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            18581..18991
                     /gene="menI"
                     /locus_tag="ACHKFK_002670"
     CDS             18581..18991
                     /gene="menI"
                     /locus_tag="ACHKFK_002670"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO9052971.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVHSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            19091..19279
                     /gene="ydiH"
                     /locus_tag="ACHKFK_002671"
     CDS             19091..19279
                     /gene="ydiH"
                     /locus_tag="ACHKFK_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO9052972.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            19828..20196
                     /gene="sufA"
                     /locus_tag="ACHKFK_002672"
     CDS             19828..20196
                     /gene="sufA"
                     /locus_tag="ACHKFK_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO9052973.1"
                     /translation="MDMPSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMIGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            20205..21692
                     /gene="sufB"
                     /locus_tag="ACHKFK_002673"
     CDS             20205..21692
                     /gene="sufB"
                     /locus_tag="ACHKFK_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO9052974.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKHNEPEWMLEFRLNAYHAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDN
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYRKKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            21702..22448
                     /gene="sufC"
                     /locus_tag="ACHKFK_002674"
     CDS             21702..22448
                     /gene="sufC"
                     /locus_tag="ACHKFK_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO9052975.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTSGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            22423..23694
                     /gene="sufD"
                     /locus_tag="ACHKFK_002675"
     CDS             22423..23694
                     /gene="sufD"
                     /locus_tag="ACHKFK_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO9052976.1"
                     /translation="MAGLPNSSNALQQWNHLFEAEGAKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLDGLTNSQFVSIAGEIAPQQRDALALTLDAVRLVFVDGRYVPALSDATE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTVRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALCDEGLKQQVLARIGQRLPGGAR"
     gene            23691..24911
                     /gene="sufS"
                     /locus_tag="ACHKFK_002676"
     CDS             23691..24911
                     /gene="sufS"
                     /locus_tag="ACHKFK_002676"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO9052977.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            24924..25340
                     /gene="sufE"
                     /locus_tag="ACHKFK_002677"
     CDS             24924..25340
                     /gene="sufE"
                     /locus_tag="ACHKFK_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO9052978.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDKSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            25489..26493
                     /gene="ldtE"
                     /locus_tag="ACHKFK_002678"
     CDS             25489..26493
                     /gene="ldtE"
                     /locus_tag="ACHKFK_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO9052979.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYNYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(26556..26792)
                     /gene="lpp"
                     /locus_tag="ACHKFK_002679"
     CDS             complement(26556..26792)
                     /gene="lpp"
                     /locus_tag="ACHKFK_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO9052980.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(27103..28515)
                     /gene="pykF"
                     /locus_tag="ACHKFK_002680"
     CDS             complement(27103..28515)
                     /gene="pykF"
                     /locus_tag="ACHKFK_002680"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO9052981.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQSFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(28523..28717)
                     /locus_tag="ACHKFK_002681"
     CDS             complement(28523..28717)
                     /locus_tag="ACHKFK_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9052982.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHNHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            29073..29282
                     /gene="fumD"
                     /locus_tag="ACHKFK_002682"
     CDS             29073..29282
                     /gene="fumD"
                     /locus_tag="ACHKFK_002682"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO9052983.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         7561 a         7143 c         7388 g         7271 t
ORIGIN      
        1 gacagctgat agaaacagaa gccactggag cacctcaaaa acaccatcat acactaaatc
       61 agtaagttgg cagcatcacc gccagcctca tccatgcggc agagatcgcc gctgtaatac
      121 cagccttctt catccagcgc acgggcggtt aattcaggtt catcaaaata ccccataaac
      181 acattggggc cacgcgaggc ttcttcacct tcgcaacctg gcggtaaagt cttgcgtgcg
      241 tcatcgacca ctttaatctc tacacctgcg gcagcgtaac cgtcggtgtg cataaagcgc
      301 ggcaaaggat cgtcgagatt caccaccgca tgcggcgaac tttctgtgga accataaaca
      361 cttaataatt taatgccgcg ctgctggcat tctcgcgcca cttttttggg gattgtggta
      421 cctccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg gttgtttctc tagtaaattc
      481 aaaagatcat agacaaacgg cgttgcgccg agcatacagg tgcaacgctg ctgctcaagc
      541 agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc accaatcaaa
      601 aaaggtgctg ttacgccatg cagaaagccc gttgcgtgac caagtggcgc aggcatcata
      661 aagacatcct gccaggtcag atttagccgc gcgcaataag cccgctcact ggcgagaata
      721 ttgttatgcg ttagcatcac accctttggc agaccctcgg ttccggaggt aaatagcaca
      781 gcggctaatt catcggcgtg ggtcgttatc gccgtggtca gaggagtatt gtcggcgaga
      841 atctgactta atgagaggga agaggtggcg ggagtcagtt tgtccacgcc gacaatttgt
      901 tgtagttgtg gaagctgatt ttgcagcggc aggattaaat ctaccggacg cgtttgttta
      961 aacaacgtcg gtgcaaagaa catttttgcc tgacacttat tgagcaccca caccagttct
     1021 gcttcccgcc aggaaggtaa cagcggcacg gaaaccgcac cggttttcag gcaggcaaga
     1081 tagataacgg taaattcaca ccagcctggc agttgaaatg cgatgcgatc acctgattca
     1141 atacccttcg ctaacatcca gtttgccaga cagctcgcgg cgtgatcgag cgcgctatag
     1201 gtgtacgttg caccatgatt gtcgaccacg gcaattttgt ctggcatcgc acgagcggtc
     1261 tgctgccagt aatctgccag tgaagcatcg ccccataacc cttgctgacg atacgccgca
     1321 cgacgttgtt cgttgaacgt taatgtcact ttcattttgg actctcgaaa cagaacatca
     1381 ggcccgagat gcgggcctgt gggatgcatg attagccgta gcgaaattca ataccgaagg
     1441 tgcctgcggg atattgccac tgttcgagaa tagtgttgcc gcacagcacc cgacaggtgc
     1501 cgcactccag acaaccggcg gaatcgaaat gaatgtttcc tgcgtcatcc tgcttataaa
     1561 gtccggcagg gcaagctttc attaatttac ggaattcatt gatatcggga tttgccgcca
     1621 aaatgatatg cgggtggccc tcatcaacat ggaatttatt gacgcctaat ttgatgtcga
     1681 cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc ttaatgccat ctttcagcaa
     1741 gttgatcagc ccaattttct tcgcgtgtcc catgatcatt ttgcgtaccg gctggtttgg
     1801 tttgccgtca atggtgaaca tatcgttcat gatgtcggcg accattcgtg ggtattggct
     1861 aaacaggcgc gggttttcca tcagcgccgg gatcttgcgg aaatgctgca tatcgcgcat
     1921 aacacaactt tgttccagct cacgtttgta ttgcgccaga ctgctggcgg agaaatccgc
     1981 gcgttctttg gcggcgatca ccgttgtggc ggcagcctga gctgatgcaa tggctaaatc
     2041 catgccgtga actgtaaaac ccaaattcag gcagaagcct gcggcgtcac caacgatcat
     2101 cacgccatcg ttaaccagct gcggcaccat tgccagaccg ccctccggca ccatatgcgc
     2161 ggaatattca agcagtttgc cgccgttaat cagcgggcga atggcggggt gttgtttaaa
     2221 atcttccagc atttgcggca cgcttttttg cgcatgggcg atatcaccca atccacaaac
     2281 cagccccaac gatacggaat ctttgttggt atagaggaat ccgccgccca tcaggccgtc
     2341 agaaggggaa ccggcaaaca gccaggcggc accttcctcg cccgtaacat taaagcgatc
     2401 gttgatctgt tctggtgtca ggcctataac ctctttaaca ccaacagcgt aatgatgcgg
     2461 atcggaagcg gaaaccattc ccagcgagcg gccaagcatc gagttaacgc catcagccag
     2521 aatcaccaca ttcgcttcga gaatatcatc cccggcctgg acgccagtga ccttgtttcc
     2581 ttcacgaacc agcgcatcga cgcgtacgcc cgggataaac tgcgtgcccg cctgctcggc
     2641 ttgttccatc aaccacgggt ccagacgatt acgcaatacg gtataagacg cgtgttgcgg
     2701 aacatctggt tgctcgcggt gaaaatcgag ggtaacggcg ctttcttcgg ttaagaagga
     2761 gattttctcg cgtgtgacct tgcgttctat cggcgctgat gctgcaaagc ctggaataat
     2821 tgcttcgagt gtgtgggcat aaagacgccc gccggtcatg tttttacatc cggcactgtc
     2881 gccgcgttct atcaccagga catccagccc ggctcgcgcc atgaccagtg cggcaacgct
     2941 accggcaacg cccgcaccga ccacaatggc atcaaatttg tcatccgaca tgcgttatgc
     3001 tcccttcccg ctgcgtttac agcgggagat gtggttgaaa gggaattagc gactcaactg
     3061 gctaatcagg gcagggacga ctttgtagat atcgcccacc agaccgtagt cggcatagtt
     3121 gaagattggc gcatttttat ctttattaat ggcgacaatc acttttgcgc cgttgccgcc
     3181 aaccatatgc tggatctgcc cggagatctc cagcgtcagg tagagatcgg atttcagcaa
     3241 cacgccggag acaccgatat aacgttcacg ctccatccag ttctcgcctt cggcaattgg
     3301 acgtgaacag ccgacttcag cattcagcac cgccgccagt tcgtggacca ttttcaggtc
     3361 atcctgcgcc gccagaccac gaccgacgcc aaccacacgt tttgctttgc tcaggtccac
     3421 gctgcttgcc gctttcgcac ggcgttcgcg acagagaatt tcatgacgcg gggcaacata
     3481 aggtaccgtt tctgtcgggc actgatgaga gatatcactg gtgcacggct cctgaacacc
     3541 gggtgcaagg gtaatgatcg ccagcgggct gttgatcttt tcctgagcga acgctaaccc
     3601 gccatacatc cggtgttcgg cgcaaatgtg accatcgaca atatccacag ccgtggcatc
     3661 gttcaccagc gccgcattca gttgcacact taaccgtgct gccagagctt taccacgttt
     3721 cgtcgcggcc aacagcaaca tagcggggtg tttatctttc agcagggcag caatgctttc
     3781 ggcgtaattt tcagtacgtt gcagcgcgtc gttttgctca agaacataaa tgcattttgg
     3841 cccataaggc ataactgcct gcgcctgctc ggtattttgt acaatggcat acacctgttg
     3901 gccccattgc tgagcgccgc caaacagttc agcataacgt tcaggattat cgctaaagac
     3961 ccagacgctg tttaattgac tcatcgtttc ttctccgtag gcttcagttc agggctttct
     4021 tcagatgttc cgccagctcg gcaatggcct ccggcgaatc gttatcgaga atgatgtgct
     4081 tacgttctgt ttgcggcggt acgcgaatgc cggtcagttc agcaagtggt gcgctctggc
     4141 tccagccaat atcacttgcc tgccactgat ttaccggttt tttacccgcg ccgagaatgg
     4201 ctttcatcga aggaatacgt ggcacgttaa tatcggaggt gacgcagagc acggctggaa
     4261 cagagagttc aataacttca acatcatctt caagcgtgcg ttcaatcacc agtgtattgc
     4321 cctgacgctg aatggcactc actgcattaa tcaccggaag ttgcagaatt tctccgacca
     4381 gcaagccaac ctgctgggca taaaggtcgc cggaaccttc accaaagatc agtaaatcga
     4441 agccgatctt ttcaactgct gtcgccagcg cctttgcggt attgagaggc agtgcatgct
     4501 caagttgtgc atcctgcacc atatacaggc tgtgcggccc gcgggatagc acgtctttgc
     4561 gcactttcga gttctgcaac aaagagccgc cgacggtcag cgcggctatc tcatcgtcat
     4621 ctgttgcaag ctggcttgca gcttcaatgg cattgagatc gaactggctg attttggcgt
     4681 cggcattgtc gaaattcagg gtgtattctg gagtgacaac aatgtcctgt tcttcaggca
     4741 ccagcttaaa gcaggttatt attttcatga tttctcctgt gagtcgcata cggaaccatc
     4801 tccgatggaa ggtaaaaatg gcctgaaaat agtgtgcgat agcggtgcgg ggttttcgac
     4861 ggagcaagct gttttccttg ccaacaatct tgcaaaaaaa atgcgatcgc taaaacaaaa
     4921 gtgcaatttg cgggaatata gttattccat gaccgtgcaa tcttgcattc agtgtaaata
     4981 atcttgttta gctctcgcta aacgcggttg tataaccgca atacacgccg tattttatcc
     5041 ggcatatatt gtttttgttt gctcatggaa tagggaggtt gtcatgtatc aacgctgttt
     5101 tgataatgcc agtgaaacgc tgtttgtcgc tggtaaaacg ccacggcttt cacgtttcgc
     5161 atttagcgat gatccaaaat gggagtcagg acatcacgtt catgacaatg aaaccgagct
     5221 gatttacgtc aagaaagggg tagcaaggtt taccatcgat tcttcgttat atgtcgcgca
     5281 tgccgatgac attgtggtga tagaacgtgg ccggctgcat gcggtggcct ctgacgttaa
     5341 cgatccggca acgacgtgta cctgtgcgct gtacggcttt cagtttcagg gggctgagga
     5401 aaatcagcta ctgcaaccgc attcttgccc ggtaattgcc gcagggcagg gaaaagaagt
     5461 cattaaaact ttatttaatg agctaagtgt gattttgccg caaagtaaaa atagccaaac
     5521 atcttcgtta tgggacgcat ttgcctatac gttagcaatt ctttactacg aaaattttaa
     5581 aaatgcttat cgttcggagc aggggtatat taaaaaagat gttctgataa aagatattct
     5641 tttctatctg aataataact atcgcgaaaa aatcacttta gagcagttat cgaaaaaatt
     5701 tcgtgccagc gtcagttata tctgccatga atttaccaaa gagtatcgta tttcccctat
     5761 taactatgtt attcaacggc gtatgacgga agcgaaatgg tcactcacta atactgaatt
     5821 atctcaggca gagatctcct ggcgtgtggg ttatgaaaat gtcgatcact ttgccaaact
     5881 gtttttgcgc catgtcggct gttcgcccag cgattaccgc aggcaattta aaaactgttt
     5941 tgcggaacaa gaaatcctat ctgaatttcc tcaaccggta agtcttgccg gataaatctg
     6001 gcggcgcagt ttgttaagag gctgcgccgc ctgcagatta tttgttctga taatctttca
     6061 ggatctgccg acctgctacg taaatcataa tttcgtctgt accgccgccg atacgttcac
     6121 aacggacatc acgccagaag cgggagacgc gcgcctcatc ggtatagccc agaccgccca
     6181 tgatttgaat cgcatcatca atgacttcca ttgcggtacg tgcgcagtac agttttgcca
     6241 gcgccgcgct ggtgcgcagt gactgatgct gatcggcttg ccatgccact ttcaacacca
     6301 tgttgcgcat gttgtcgatc ttaatcgcca tcagcgccag tttttcctgg atcatctggt
     6361 tatgaccaat gggcttacca aaagcgatac gctggttggc atagggggcg gcatcttcga
     6421 atgcgcattc ggcaaagccg gtgctgcgtg cggcgttgat caggcgctcc atctcaaagt
     6481 tgtacatcac attgaggaaa cccattcctt cttcgcccac catgtcgctc tcttcaactt
     6541 ccacgttgtc gagatagact tcgcaggtgc tgagcatatg ccagccgatt ttatgcagtg
     6601 gattgatttt aatgccgggt ttactggagt cgacccacca cagagtaaag gcctttttcg
     6661 gatctttcgg ttgcggatcg cgcgccaaca ccagcatata tgggtactct ttcgcaccgg
     6721 taataaaggt tttctgtccg ttgatgtaaa ccttgccgtt tttacgcgtg taagtggtag
     6781 tggcactgtt gttgtctgag cctgcgcctg gctccgtcaa tgccagggca taggcgggat
     6841 cacccgtttc cagggtgctc tctgccgttt tacgtagctg ctctgcagaa ccgaaacggc
     6901 gcatactgtg aatacattga ccgttggtaa tcaaaaatgc cggagcaccg cattttgaca
     6961 cttccatcag cgccagcatt tgggtgacgt aatccgcagg gataccacca aattcttccg
     7021 gcacgccaag catggaaata ccgttatccg ccagtgcccg cataaactca cgcgggtatg
     7081 tcccgttttg atcgcaggtg cggaaatact cttccggaaa gttagtcgta atcagttcgc
     7141 gaatactggc cagcagcagt tcttgttctt cagttaaaga aaaatccatc attatactcc
     7201 tgttaatgag ccgcttcagg caggacaaaa cccatcgccg catcgataaa taatctttcg
     7261 tccatcagtt tgagttctgg cgaaatcact ggggtgaaat ccattttgtc gagaatatct
     7321 ttttgtaaat cgacgccagg agcgatttcg attaaatgca ggccgtcttc ttttaacgtg
     7381 aatacggcgc gctcagtgat ataacgaaca tccagccctc gctcgagagc gatttttccg
     7441 ctgaaagtta tttccggtag ttcccgaata aatttcttca cccgtccttc ctggacgata
     7501 tttaatttgc cgtcggtaat ttctgttttt aaactgcccg cagttaatgt gccgcagaac
     7561 acgattttct tggaagtggc actgatatca ataaatccac cagtgcccat gattttacca
     7621 ttgaatttat gcacgccgac gttaccgtgc tggtcgactt cagcaaaact caaataacaa
     7681 acatccagac caccaccgtg ataaaaatca aactgggacg tcatatccag aatggcgcgg
     7741 gtattcacgt tcgcgccaaa ggcgatcccc tgtgaagtaa taccgccaat cggacctgtt
     7801 tctaccgtca gaataaagtc atcagcacaa ccttcttccc gggcgaccag gccaatgccg
     7861 tcagcaatac cgacgccgac attccccact gcgcctttgc gcatttcgaa taatgcacgc
     7921 cgtgcaacta atttacgttg gtttaggggc aggctaagtt tggtgctgtc atcaagagtg
     7981 aagtcaccag aaataaagcg gttaaccggc gcaccgccat acagttgggt ttgatccgga
     8041 tcgaccacca caatatccac cagataaccc ggaatacgga cagatttagg atgcagcgtg
     8101 gctttcttaa ccattttctg cacctgcatc atcacaatac cgccgttatt gtgcaccgcc
     8161 tgagcaataa ccaatgcgtc gagatacatc acctcatctt caaaagtggc gtagccttcg
     8221 ctgtcgcagg tggtagcgcg aatgaaggcg atatctggcg ctatcgcttt gtaatagaga
     8281 tattctttgt tatcaaactc gaccagttta atcagttctt ctttagtgac ttcattcagt
     8341 ttgccgcctt gctggcgtgg atcgacaaat gtcccgatgc caatatcact aataatacaa
     8401 ggctggtggg ctgcggcggc gcgtaaggtt tgtgtaagta caccttgtgg atagttataa
     8461 gcaataattt tattttgttc tgcgagatca gaaatacgcg gcgattgtcc ccagtgaccg
     8521 cataatgccc atttcaccag accttcttgc gccagcggac taataccacg gtcggcgcga
     8581 tcgccaagcc ctgttggact aataatcgat aaattacgtg gtgtttgagt ctgtttatat
     8641 ttatcagcaa gagcagtaat taacgtggtg gcttccagaa taccgccgcc agcgcctaac
     8701 acacaaagtg ttgcttcgtc gggaatataa ttcaccgctt cctgtgccga caggaccggc
     8761 actcgtccat taatacgagg tggttttaca ggtttcattg ttaattcctg attcaggtgt
     8821 gattttccct acgccagatg gagaattagc gcacagagac tcacgaaata tttttattaa
     8881 atacggttaa aaatactctt tttatttcgg cgggagaggg tattcccgcc gaaatattat
     8941 tgcttatgcc tgatgtaaaa tagttaatac cgtacgcaaa tcatttaccg agatttgccc
     9001 tggcgcagag gcttttttta ccgcaccaaa agttgccgcc gacccaaata cttcaccagc
     9061 cagacgagaa attacgccag tttttgccat cgacatcgtg atgattggac gatcggcata
     9121 ctgttcctgc atctccaggg tcgcggcaag caacgtcagc acatcgctgg tactttgcgg
     9181 catcagcgca atcttaggaa tatcggcgtc gaaggactgc attttgcgca gacgggcaat
     9241 gatttcttcg gcttccggcg ttttatggaa gtcatggttg gacatgacaa ctttcacatc
     9301 atgcgcgtgg gcgtaggcga cggtttcttt aacctgatca tcgccggtaa ataactccag
     9361 atcgatcata tcaaccaggc cactgtcgat ggctgcacga ttgagtgcaa tataagcctc
     9421 ggtggaaatc gcctgctcgc cgccttcttt ggcactgcgg aaggtaaaca gcagcggttt
     9481 ttctggcatg gtttcacgga gaatttttgc tgccgctatg acagactcca cattggagag
     9541 atcggcatag tggtccacac gccattccag aatatcaaag tccgcttcac gataggcgag
     9601 agcttcggat ttcacgctgg cgatatcttt cgccatcagc gagacgatga ttttaggtgc
     9661 gcccgtacca atgacgagat cttttacagt tacggttttc attttttacc ctttctgcac
     9721 gcggtcagcc tgtcaggcac cgaaccccat gacctgttta acatattcca gagggaaatc
     9781 tttgccagtc cataaagtga actgttcagc cccttgccac aacaacatgc cgtatccatc
     9841 aatcgttttg caacccgctt gttgcgcctg ctgcaataac ttcgtcatat gcgggttata
     9901 cacgcattca gtgaccagaa gtcccggatg taacagactg atatcattaa ccaatgattc
     9961 attctcaagg ggtttcatac ccactttggt gccattggtt aaaatgtcgg cggaagccag
    10021 ggcttcagca aaggcttgct gatcggcgag atcggtgact gtcacgacgc aatcggtgtt
    10081 ttcgttaacc cgctgcgcga aggcgagggc tttatcgaag aactcatccc gacggttaaa
    10141 gagtttaatt tcttttaaac cttcaattgc cccctgcgcg ccaattgccg ttgaggcacc
    10201 tccggcccct aacagcacca tcgttttgcc tttgatatca aaaccgctct ctttaatggc
    10261 gcgaatatgg cccgtgccgt cggtgttata gccacgcaga tagccatcat catttacgat
    10321 ggtgttgatg gcacccacca gtttggctgc tggcgttaat tcatcaacat attcacacgc
    10381 cagttgtttg ttcggcattg atacaccagt tccgcgcatt ttgagggctt ttaatccttc
    10441 aattgctccg ggaaaactat cgttatccac ttcaaaggcc atataggtaa atggcaatcc
    10501 cgctttttct aaggctttat tctgcatttc gggcgataaa ctgtggcgga tgggataggc
    10561 catcaaccca atcaattcgt attttgcggt aacatccata attaactcct ttaacctcta
    10621 tgcttaatcg tgtttaaccg ggtgctaaat ttatgctcgc caaaccgcac atcattttca
    10681 ggaataatga aaacgcggta atagcggata aaaacaataa ttgcggtaat cagggccagc
    10741 agcgcgaaag taaaatcgag aacaatgata tattgcaggc cgatgttcga cagataaccg
    10801 gtaatcagcg gaataacaaa gttagccagt ccacccatca tcatatatat actggtgact
    10861 ttggctttgc ttttggggaa aaactctgac atgaccgaaa cgccgagctg taaaatgccg
    10921 ccagctgctg agaaaccgat aacaaaggct ccggcattac acaccaatgg agaagggtac
    10981 aggtagataa tggcagctgt tattgttgcc agtgcagagt taaatacatt agcccagatg
    11041 ggacggacca tttttttcag tagtgcggca aaaataaaga cacagaccag cgaacccata
    11101 ctgtaataag agatggtttt taatgcctca gcttctgaca taccagcaaa agccatcgca
    11161 tatttgggca tccacaccac aatcacataa aaggtcgaga atgcggctac accgaacagt
    11221 actgatgaaa caccttccag ccagactaac ggtttgctgt tcatttgcgg taattcatta
    11281 gctacgctgg cgtccaccaa ctggctgggg aatttgcttt tcaacagcat cagcgtgatc
    11341 agtacaaaca gaatacccgg aataatcagc ccatagccgt accagatgtt attgagcaac
    11401 atatagctca ccagcattgg gtagaacatt tgcccgaatg acaccatcgc tttaaccagt
    11461 atgaccgccg aaccagaggc tttcggaaag cattccatga gcgcggggta gccacccgta
    11521 tccagcgctg agttagcgat acctacgcac actgccagac agtaggcgag agttaaattc
    11581 gggcaagcag gaataccaaa gaagaatagc agatacatta ttactgccat taatatcacc
    11641 gcccgacgac caaacttatc ggagatcact ccgaagaata aaatactgat caatcgcccc
    11701 aatccgatac cggaaattaa ataggcaatg cccgcgttgt cagtggaaaa cttttctgcc
    11761 aaagatgtca tgttttgggc aagcgtaata acactaatgc cgtgcaggaa atagctgaag
    11821 taaatacaaa gaacagccag gataaatggc gtgctgaaag ccttattttg agacattttc
    11881 acagctcctt gctgaaagat aatttcgtta ccgtacgtat tgttctccga cggtttacat
    11941 aattactata ctgaagatga ttttccctgg gtacatggca taaagtttat gtttccagga
    12001 atattgcatg tcatagtttg tttttgatcg cgattttcct tagttagcgc agaaagagtt
    12061 atttatccgt aagatcttgc ataacctgaa aagaatattg cattacccac ccggagcgat
    12121 attttccttt agcgtgtgat gccgcgtttc tttgcgactg cgtaagccaa taaacagtgc
    12181 cagtataaag ccgatagcag cgatggcggt atcgaaccac ataatatcgg caatacttct
    12241 ttgcgaaaga tgagccgtaa tcaacggaat ggtaaatgtt gcaatgctgc cagcactgta
    12301 atagatcccc gttgcttttc ctttagcgta gggaaaacgt tccgccatta acgtcaggcc
    12361 aatttgtaca acaccgcctg cggaggtaaa accgatgacg aaagcaaata ttatcaccac
    12421 ataaaatgtg ggatgcaggc agacggtgag cagggcgata aatgagataa aggtgtacag
    12481 catcagtaat gttgttgggc gaacggtatt acgaataagt ggagcagtaa taaatacaca
    12541 cagcagcgaa cccacggtgt agatactgag taatttgatc gacatggtgt atgacatgcc
    12601 agcgacaaat tgtccgtatt gtgccagcca ctggctaacc agataaaacg ttgccattga
    12661 gatatagcca tatagggaat aactggctaa atcgataatt gaacagcgat gttccgtaga
    12721 gctggtggtt ttctttatga caggtaagtg acgacccgga tgaggtggga acgtacaacg
    12781 gtataaaaac agagcgttaa taaacataat gcctgcggca atcataaagg accaaccgaa
    12841 ccacagttca gcccacacta acaggctgat aattagcggt aataaaaact gtccgctgga
    12901 aacaaatgct ttaattaaaa tattggctgt acccggtgag cgtggaaaag cttccatcaa
    12961 actgggatag gtgcctgcat cgagaaaact gtttgccatt cccgccagaa agccaaaaac
    13021 ataagcgata atgatgttat tggtgtgcag gatgccaaaa aagaaggcca tatagcagca
    13081 catcccgagc atgataaaag ggcggcgacc aaagcgatcg gataataatc ctgcaaaaag
    13141 caagacactt aatcgaccaa tgcccagtga tgagataact atcgagacgc ccgcggcatt
    13201 agtttgccaa agcgtctcca gcgaggccat attcaggctc atcaaaatga cgcccatacc
    13261 atgcaccagg taattaaaat acaacccaag tgcggtaggg taatagggat ttttcatagg
    13321 ttctacttag caaaggtgct ttaaaaagtg tagcgatgtt gtgtcagtat ccgttcatta
    13381 ttgttgtaac aacgacaatt cctttctgtg gagaaattaa caaagccgct ccagatcgtg
    13441 tgcatataat tcagcggcta ccgattgata gatcttccag tcaataaaat tgccatcagg
    13501 gtagacttgt tgaaacgcgg ttaagtcggg gaaaaaacgc atggtgttgt tgccgatgcg
    13561 gttatttatt ttctctatga tggcatgaag atgttttttt cgttgctgac gcatagccaa
    13621 cagttgctcg actacacaat caggaatggc gcatttgcca ttttcccact gacgccagct
    13681 ttcactgtta ccggtctggg caatccaggt cgcgcattca tcaatagtca tggcaaaaat
    13741 atggcgaagt gcctggagtt cataagcgtt catcgtgtaa tggctgcatg aaataaacaa
    13801 taaaatataa ataacattat cagcctgctg acggcttgaa gagagtcaca gtatcttgtg
    13861 caacattatt ggaaagagtg aggggcgagg tagcgaagcg gaaaaatgtt aaaaaaaagc
    13921 ccatcgtggg agatgggcaa agactacaca cagcaattcg ttgtttcact caggggattt
    13981 ccatgcttat aaatcaatat gttgatttat aaccgtgagc taatagtagg catacggact
    14041 ttttgcgtcg atcagattcg tctcattagt ttaaaaactg taaagaattt gcgacaggta
    14101 ctaccagctg aatagcggta agcaggaaaa tatggggaag agcaagcaaa tatcaattag
    14161 caggggttaa caatcaggcg gcagagccgc ctgatgagaa attacttatt cgatttctcg
    14221 atttcgccaa gttcttcgag aatttcttcc ggttggtccg tcggcggtgg gacttcttcc
    14281 acccacgcgg caaaaagacg ccaggaaacg gctaacagaa ccggaccaat aaacagaccg
    14341 atcatcccga aagcaatcaa accaccaata acgccagaga ggatcaggat cagcggtaaa
    14401 tcggcaccca tgcgaattaa cattgggcgg atgacgttat cgagcgtgcc aaccacaccg
    14461 ctccaaacta acaataccgt tccccaggtg gtatcgccag tccagtagag ccagataatc
    14521 gccggaatca gtaccggcaa cggtccaagc tggacaaggc aggagaggat cattaacacc
    14581 gttagcaaag ttgcataagg tacgccggat acggcgaggc cgataccgcc aagcactgcc
    14641 tgtactaacg ccgtcaccac cacacccagc gccaccgcgc ggatagcctg tgccgccagc
    14701 agcacggcgg catctccgcg aacgcctgcc agacgggttg caaaatggcg aatgccttgt
    14761 gccacctgtt caccgcgcca gtacagcagg gcgctgaaga gaagcatcag cgcacaatgc
    14821 accataaagc gcccgatatg cgccgcctgc ccaacgaacc aggtggtggt ggtgccaata
    14881 taaggacgga ctttcgccat gatcgccgtc ccccccatat ccagcaaatt gtgccagcct
    14941 gcatatagct tcgcgccaat caccggaatg gtattaagcc acgccagatc gggtaacgtc
    15001 atgtcaccac tggaaatggc tttaattagc gggccgctgc cgtcgacgat actgttaacc
    15061 agcaaagcaa tagggatgat aaacaccatc actaataaca gcgtcattac cagaacggcg
    15121 agagagcggc gaccaaacat gatcttttgc aaacgtaaca ataccggcca ggtggcgata
    15181 accactgtac cggcccatgc aaagccgaga ataaagggtt gtacaatcca cagacatgcc
    15241 acaatcatga tggctaaaaa cagaaccgaa agcagaattt gtgcgacatc cctgggctga
    15301 cgaacattta ccataactac ttttcacctt ttttgtgcgt caaatcgtcg acgcgacgtg
    15361 aacacgcgaa actcacctgc ataatgatga gcaatttcag cggtttttaa caggtcgatt
    15421 ctgcccttaa ttctgatggg cgtgttagaa aaaaatgtgg tacaacaact caggcaacac
    15481 gcaaacgatt aatctctcat cacagaccgc aggaaagggt caatataatg attccacaga
    15541 tttcccaggc acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac
    15601 agggttttac cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgactgaca
    15661 acagtattta ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtag
    15721 cgctgatcgc ccgtcttgcc gcgcaggaac gctattcatc gttgatcttt accccccgcg
    15781 gcggcggcac cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc
    15841 gccatatgaa ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg
    15901 gggtgattaa agaccaactc aatcagtacc tgaaaccctt tggctacttt tttgcgccag
    15961 aactttcgac cagtaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc
    16021 agggatcgct ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt
    16081 tggggggcga tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta
    16141 aatccaatac cacaatcggg cgaatttata acacggttta tcaacgttgc cgtcagcaac
    16201 gccagttaat catcgacaac ttccccaagc ttaaccgctt tcttaccggt tacgatctgc
    16261 gtcatgtctt taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag
    16321 aagggacgct ggcctttatt accgaagcgc gactggatat tacgcgcttg cctaaagtac
    16381 gccgtctggt gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta
    16441 tggttgaggc gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc
    16501 gggaagatat tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaaa
    16561 tgctcgggct gaacattgtg gaatttgctg gtgatgatga gacgctgatt gatgagcgag
    16621 taaatgcact ctgtgcgcgg ctggatgaac ttatcgccag tcagcaagca ggtgtgatcg
    16681 gctggcaggt atgcagtgag ctggcaggtg ttgagcgtat ttatgcgatg cgcaaaaaag
    16741 ctgtcggcct gctcggtaat gccaaaggag cagccaagcc aattccgttt gctgaggata
    16801 cctgcgtacc gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca
    16861 gccacggctt aagctacggt atgttcggtc acgtcgacgc aggtgtcttg cacgtccgtc
    16921 ctgcgctgga tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg
    16981 tggtggcgct gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc
    17041 gcgctgaata cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga
    17101 aagcggcatt tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc
    17161 tcgatgcgcc gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc
    17221 ccattgcggt acgccagcag tggcgcggtg caatggagtg taacggcaac ggtttatgct
    17281 tcaactttga tgctcgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc
    17341 attcaccgaa agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg
    17401 gcgttgaccc actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttgcggacgt
    17461 taattgcccg cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg
    17521 aagtcaaaga ggcgatgtcg ggctgtctgg cctgtaaagc ttgttcgacc cagtgcccca
    17581 tcaaaattga tgtgccggag tttcgctctc gttttctgca gctctatcac acccgttatt
    17641 tacgccctct gcgcgaccac cttgtcgcta cggtcgagag ctacgcaccg ctgatggcac
    17701 gcgcgccgaa aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa
    17761 aacatattgg catggttgat ttgccgctgc tgtcggtccc ctcgctgcaa caacaaatgg
    17821 tggggcatcg ctcggcgaat atgacgctgg aacagcttga agctctcaat gcagagcaga
    17881 aagcgcgcac ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg
    17941 tggcggattt tgtccgtctg gtcgaaaaat taggtttcca gcctgtgtta ctgccatttt
    18001 cgccaaatgg caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga
    18061 aaaagacggc ggatttcctc aaccgtatgg cgaagctggg gatgccaatg gtgggcgtcg
    18121 atccggcgct ggtactttgt tatcgcgatg aatataaact ggcactgggc gaagaacgtg
    18181 gcgagtttaa cgtcttactg gcgaatgaat ggctggcaag cgcacttgag tcacagccag
    18241 tggctacagt cagcggtgaa tcatggtatt tctttggtca ctgtaccgaa gttaccgcct
    18301 tgccgggcgc gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa
    18361 atgtcagcgt gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccata
    18421 aaaattcgct cgggatctat gagttatcct ggcatcaggc gatgcagcga ctaccgcgta
    18481 accgctgtct ggcgaccgga tattcctgcc gtagtcaggt aaaacgggtt gaaggcacag
    18541 gggtacgcca tcctgtgcag gctttactgg agattattaa atgatatgga aacgaaaaat
    18601 caccctggaa gcactgaatg ctatggggga aggaaacatg gtgggattgc tggatattcg
    18661 ctttgaacat attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa
    18721 gcagcctttc gggttgctgc atggaggggc atccgtggta ctggccgaaa gtatcggttc
    18781 cgttgccggt tatttatgta ccgaaggtga gcaaaaggtg gttggtctgg aaatcaatgc
    18841 taaccacgtc cactcggcac gagaagggcg ggtgcgcggc gtatgcaaac cgttgcatct
    18901 cggttcgcgt caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg
    18961 ttcgtcacga ttgacgaccg ctattttgtg acgttgtcac cagaaaagtg tgacgcatat
    19021 tgcttttggt tattatctgc acagtaaacg gtggtatact gtgcaggtct tgtctaaaag
    19081 cgaggatgct atgtctactc aacttgatcc cacccaactg gccattgaat ttttacgccg
    19141 tgaccaaagc aatctttcac ctgcccaata tctcaagcgt ctgaaacaat tagaacttga
    19201 atttgctgac ctgcttactc tctcttctgc tgaattaaaa gaagaaatct acttcgcctg
    19261 gcgtctgggc gtgcattgat cctgatttaa tgtcgcgagt tcgccaaatc ggcctggatt
    19321 aatcttcgtc caggttcgag ggccttttta atacagccta tctacccgcc tgataacgtt
    19381 caaaaataat acaaatcgca gtttgtgtta aaacagtggg ttagctttat gagattatcc
    19441 ttatcgcccc ttcaagagct aagccactga gagtgccgga gataagcgcc ggatggggta
    19501 gaaaccctta agcctgtgtc gcacagactt aagggttttc ttatttctga acgctgcact
    19561 tttttaccta tctcttaaat agtcattttc aatacaactt tttctttttc atcacagcct
    19621 taatgctcct tagataataa tcattatcta acaatgagat acctaattct tagcgtgcct
    19681 gttaacccac acatcagggt ctatgcttat taaaagcagg taaaaagcat gttttacgct
    19741 aaagcccctg cgtttgctgg gttgaactga taatcattat cactaacatg ctgttatacg
    19801 ctgaaagcga tgaagtgagg taaatcgatg gacatgcctt caggaacctt taatccacag
    19861 gatttcgcct ggcaaggctt aacgctgaca cccgcagcgg cgatacacat ccgcgaactg
    19921 gtggcaaagc agcctggtat gatcggcgtg cgcttaggcg ttaagcaaac gggctgcgcg
    19981 ggctttggct atgtgctcga cagtgttagc gagccggaca aagacgatct gctgtttgaa
    20041 cacgacggcg cgaagctgtt tgtcccgctg caagcgatgc cttttattga tggcacggaa
    20101 gtcgatttcg ttcgtgaagg acttaatcag atattcaaat ttcacaaccc taaagcccag
    20161 aatgaatgtg gctgtggcga aagctttggg gtataggcgg tactatgtct cgtaatactg
    20221 aagcaactga cgatgtcaaa acctggaccg gcggcccgct gaattataaa gaaggattct
    20281 tcacccagtt agccaccgat gagctggcaa aggggataaa cgaagaggtg gtgcgcgcaa
    20341 tttcggcgaa gcataatgag ccggagtgga tgctggagtt tcgtctcaac gcctatcacg
    20401 catggctgga gatggaagaa ccgcactggt tgaaagcgca ctacgacaag ctgaattatc
    20461 aggattacag ctactactcg gcaccatcgt gcggtaattg tgacgacaac tgcgcgtctg
    20521 aacccggcgc ggtgcagcaa actggcgcga acgccttttt aagtaaagag gtggaggcgg
    20581 cgtttgagca gttgggcgtt cccgtgcggg aaggcaaaga ggtggcagtg gatgccattt
    20641 tcgactctgt ttcggttgcc actacttatc gcaaaaaact ggcagagcag ggaattattt
    20701 tttgttcctt tggcgaggcg atccacgatc acccggaact ggtgcgtaaa tatctcggca
    20761 ccgtggtgcc ggggaatgac aacttctttg ccgcacttaa tgcggcggta gcctctgacg
    20821 gtacgtttat ttatgtgcct aaaggcgtgc gttgcccgat ggaactttcc acctattttc
    20881 gcattaacgc ggaaaaaacc gggcagtttg agcgcaccat tctggtggcc gacgaagaca
    20941 gctacgttag ctacattgaa ggctgttccg ctccggtgcg tgacagctat cagttacacg
    21001 cggcggtggt ggaagtcatc atccataaaa acgccgaggt gaaatattcc acggtacaaa
    21061 actggttccc tggcgataac aacaccggcg gtattctcaa cttcgtcacc aagcgtgctt
    21121 tgtgcgaagg cgaaaacagc aaaatgtcat ggacgcaatc agaaaccggc tcagcgatta
    21181 cgtggaaata tcccagctgc attttgcgcg gcgataactc cattggtgag ttttactcag
    21241 tggcgctgac cagcggtcat cagcaagcgg ataccggcac caagatgatc cacatcggta
    21301 aaaacaccaa atcgaccatt atctcgaaag ggatctctgc cggacatagt cagaacagtt
    21361 atcgcggctt agtgaaaatc atgccgacgg caaccaatgc gcgcaatttc actcagtgcg
    21421 actcaatgct gattggcgct aattgtgggg cgcatacctt cccgtatgtc gaatgtcgca
    21481 ataacagcgc tcagctggag cacgaggcaa cgacatcacg tattggtgaa gatcaactgt
    21541 tttactgcct gcaacgcggg atcagcgaag aagatgccat ctcgatgatt gttaacggtt
    21601 tttgcaaaga cgtgttctcg gaactgccgc tggaatttgc cgttgaagca caaaaactcc
    21661 tcgccatcag tcttgaacac agcgtcggat aaggaataaa catgttaagt attaaagatt
    21721 tacacgtcag cgtggaagat aaagctatcc tgcgtggatt aagcctcgac gttcgtcccg
    21781 gcgaagttca cgccattatg gggcccaatg gttcgggcaa aagtacctta tcggcaacgc
    21841 ttgccgggcg agaagattat gaagtgacga gcggcacggt tgagtttaaa ggcaaagatt
    21901 tgcttgcgct gtcgccggaa gatcgtgcag gtgaaggcat ctttatggcc ttccagtatc
    21961 cggtggaaat tcccggcgtc agtaaccagt ttttcctgca aacggcactt aatgcggtgc
    22021 gcagctatcg cggccaggaa acgctcgacc gctttgattt tcaggatttg atggaagaga
    22081 aaatcgctct cctgaagatg ccggaagatt tattaacccg ttcggtaaac gttggttttt
    22141 ccggcggcga gaaaaagcgc aacgatattt tgcaaatggc ggtgctggaa ccggagttat
    22201 gcattcttga tgagtcagac tccgggctgg atattgacgc attaaaagtg gttgccgatg
    22261 gcgtgaactc gctgcgtgac ggcaagcgtt cattcatcat tgttacgcac taccaacgca
    22321 ttctcgacta catcaagcct gattacgtcc atgtgctgta tcagggacga attgtgaaat
    22381 ccggcgattt cacgttggtc aaacaactgg aggagcaggg ttatggctgg cttaccgaac
    22441 agcagtaacg cactgcaaca gtggaatcat ttgtttgaag ctgaaggggc gaaacgctcc
    22501 ccgcaagcac agcagcattt acaacaattg ctgcgtaccg gactgccgac acgtaaacat
    22561 gaaaactgga aatatacgcc gctggacggg ctgaccaata gccagtttgt cagcattgcg
    22621 ggggagatag ccccacagca gcgtgatgcc ttagcgttaa cgttagacgc cgtgcggctg
    22681 gtgtttgtcg atggacgtta cgtgcccgca ctgagcgatg caactgaagg cagcggatat
    22741 gaagtgagca ttaacgacga ccgtcagggg ttacccgacg ctattcaggc ggaagtgttt
    22801 ctgcatttga cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa gcgcggtcaa
    22861 cggccggcaa agccattgct gttaatgcat atcacccagg gcgtggcagg tgaagaggtg
    22921 aatactgccc attaccggca tcatctggat ctggcggaag gtgcagaagc aacggtgatc
    22981 gaacattttg tcagccttaa tgatgctcgt cacttcaccg gcgcacggtt cactatcaac
    23041 gtcgcagcga acgcccattt gcagcatatc aagctggcgt ttgaaaaccc gctcagtcac
    23101 cactttgctc ataacgattt gttgctggct gatgatgcca ccgcatttag ccacagtttc
    23161 ctgctgggtg gcgcagtgtt acgacacaac accagtacgc aactcaatgg cgaaaacagc
    23221 acggtgcgga tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga tactcgtacc
    23281 tggctggaac acaataaagg tttttgtaac agccgacagt tgcacaaaac tatcgtcagc
    23341 gacaaaggcc gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc catcaaaacg
    23401 gatggtcaga tgaccaacaa taatctgctg atgggcaaac tggcggaagt ggatactaaa
    23461 ccgcagctgg aaatctatgc agatgatgtg aaatgcagcc acggcgcgac ggtggggcgt
    23521 attgatgacg aacagatgtt ttatctgcgc tcgcgcggga tcaatcagca agatgcccag
    23581 cagatgatca tctacgcctt tgctgccgaa ctgacggaag cactgtgtga tgaggggctg
    23641 aaacagcagg tgctggcccg aatcggtcaa cgactgccag gaggtgcaag atgacttttt
    23701 ccgtcgacaa agtgcgggcc gactttccgg tgctttctcg tgaggtaaac ggtttgccgc
    23761 tggcttatct cgacagcgcc gccagtgcgc agaaaccgag ccaggtgatt gatgccgagg
    23821 ctgagtttta tcgtcatggc tacgcggcgg tgcatcgcgg tattcatacc ttaagcgccc
    23881 aggcgaccga gaaaatggag aacgtgcgca agcgtgcttc gctgtttatt aatgcccgtt
    23941 cggcggaaga gctggtgttc gtccgcggca cgacggaagg gatcaatctg gtcgccaata
    24001 gctggggcaa cagcaatgtg cgagcgggcg ataacatcat catcagtcag atggagcacc
    24061 acgctaacat tgtcccctgg cagatgcttt gtgcacgcgt tggcgcagag ctgcgtgtga
    24121 tcccgctcaa ccccgacggt actctgcaac tggagacgct gcctacgctg tttgatgaga
    24181 aaactcgtct gctggcaatt actcatgtct ccaacgtgct tgggacagaa aatccgctgg
    24241 cggagatgat cacacttgcg catcaacatg gcgcaaaagt gctggtggat ggcgctcagg
    24301 cggtgatgca tcatccggtg gatgttcagg cgctggattg cgatttttac gtgttctctg
    24361 ggcataaact gtatggccct accggaattg gcattcttta tgtcaaagaa gccttgttgc
    24421 aggagatgcc gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc ctgagtgaag
    24481 gcactacctg gaccaaagca ccatggcggt ttgaagccgg tacacccaat accggaggca
    24541 tcattggtct tggcgcggcg ctggagtatg tttcggcgct ggggcttaat aacatagccg
    24601 agtatgaaca gaatctgatg cattacgcgt tatcacagtt ggaatctgta ccggatctca
    24661 ctctctatgg cccacaaaac aggcttggcg ttattgcttt taatctcggt aaacaccacg
    24721 cctatgatgt tggcagtttt ctcgataatt acggcattgc tgtgcgtacc ggacatcact
    24781 gcgcaatgcc attaatggcc tattacaacg tccctgcgat gtgtcgggca tcgctggcca
    24841 tgtataacac ccatgaagaa gtggatcgtc tggtgaccgg cctgcaacgt attcaccgtc
    24901 tgctgggata acagggaggc actatggctt tattgccgga taaagaaaaa ttgctgcgta
    24961 attttttacg ctgcgccaac tgggaagaga aatatctcta cattattgag ttgggtcagc
    25021 gtctgccaga attacgtgac gaagacaaaa gcccgcaaaa tagcatccag ggctgccaga
    25081 gtcaggtgtg gattgtgatg cgtcaaaatg cgcagggaat tattgaatta cagggcgaca
    25141 gcgatgcggc cattgtaaaa gggcttattg cggtcgtctt tattctttac gatcaaatga
    25201 cgccgcagga tattgtcaat ttcgatgtgc gcccgtggtt tgaaaaaatg gcgctcaccc
    25261 aacatctcac cccatcacgc tcacaaggtc tggaagcgat gattcgcgca attcgcgcca
    25321 aagccgctgc acttagctaa actaaaggga cagctttcat ccggttggct cacattgccg
    25381 ggtggtcaat gtttacactg gcctgcacag gagtgttttg gtttcaggtg aacataagcg
    25441 ccatccgacg tttcagcgtg agtctccggc aaatacagga ggtttacaat gaaacgcgcg
    25501 tctttgctta cactcacgct tatcggcgct tttagcgcca tccaggctgc ctgggcggtt
    25561 gattatccgc taccaccaac cggcagtcga ctggttgggc aaaatcaaac gtatacggtg
    25621 caagaagggg ataaaaacct tcaggccatc gcccgacgtt ttgatactgc ggcaatgttg
    25681 atccttgaag ccaataacac tatcgccccg gtgccaaaac ccggtacgac gataactatt
    25741 ccttcacaac tattattacc tgatgcgccg cgtcagggga ttattgtaaa ccttgcagag
    25801 ctgcgccttt ataattatcc gccgggagaa aatatcgtgc aggtttatcc gataggtatt
    25861 ggtttgcagg ggctggaaac gccggtgatg gaaacgcgtg tggggcagaa aatccctaac
    25921 ccaacctgga cgcctacggc gggcattcgt caacgttcgc tggaacgtgg cattaaatta
    25981 ccaccagtcg ttcctgccgg gcccaataac ccactaggac gttacgcact gcgcctcgcg
    26041 catggtaatg gcgaatacct cattcatggc accagtgcgc cggacagcgt cggtttgcgc
    26101 gtcagttcgg gctgtattcg catgaatgca ccggatatta aagctttatt ctccagcgtg
    26161 cggacgggaa cgccggtgaa agtgatcaac gagccggtga aatattccgt ggaacctaac
    26221 gggatgcgtt atgttgaagt acatcgacca ctatcggcag aagaacagca gaacgttcag
    26281 acaatgccgt acacgctgcc agcaggcttt acgcaattta aagacaataa ggctgtagat
    26341 cagaagttag tcgataaagc gttgtatcgt cgggcagggt atccggttgc ggtgagcagt
    26401 ggagcaactc ccgcagccag caatgcgcct tcagtagagt cagcgcagaa tggggaacca
    26461 gagcaaggga atatgttacg cgcgacgcag tagcggtaaa tagcaggcaa aaaaatggcg
    26521 cacaatgtgc gccatttttc acttcacagg tactattact tgcggtattt agtagccatg
    26581 ttgtccagac gctggttagc acgagctgcg tcatctttag cagcctgaac gtcggaacgc
    26641 attgcgttca cgtcgttgct cagctggtca actttagcgt tcagagtctg aacgtcagaa
    26701 gacagctgat cgattttagc gttgctggag caacctgcca gcagagtaga acccaggatt
    26761 accgcgccca gtaccagttt agtagctttc attattaata ccctctagat tgagttaatc
    26821 tccatgtagc gttacaagta ttacacaaag ttttttatgt tgagaatatt tttttgatgg
    26881 gaaggcactt atttttgatc gttcgctcaa agaagcatcg aacgctgggt tttagataaa
    26941 aaaattgaga gaaaacagga tgcttccatc ggattcatct tagataaagt gagattatat
    27001 agtgaaatcc gatttgattt tttattgctt ctggttatcg attaaataaa aaaagcgccc
    27061 atcagggcgc ttcgatatac aaattaattc acaaaagcaa tattacagga cgtgaacaga
    27121 tgcagtgtta gtagtgccgc tcggtaccag tgcaccagaa accataacta cgacatcacc
    27181 tttgtgtgcc agaccgctct gcagagccag ttctttaccc agacggtaga agtcatcagt
    27241 agaagtgatc tctttaacca gctgcggcac aacgcctttg ctcagtacta actggtgagc
    27301 tgttttttcg ttggtggtca gtgccaggat ggtggcatcc gggaagtatt tacgtactgc
    27361 gcgagcagat ttaccgccct gagtagcaac cacgatcagt ggagcatcca gtttttcagc
    27421 agtttcaacg gcaccacggc ataccgcttc ggtaatacgc agtttacggt tgtcattgtt
    27481 gaactcgaga cggctgttca tcacgcggtc ggtacgttcg cagatggtcg ccatgataga
    27541 aaccgcttcc agcgggtatt taccttttgc ggattcacca gacagcatca ctgcgtcagt
    27601 accgtcgagg atggcgtttg caacgtcacc ggcttctgcg cgagtcgggc gtggattttt
    27661 gatcatggaa tccagcatct gggtcgcagt gataacaact ttacgtgcac ggatacattt
    27721 ttcgatcatc atcttctggg cgaagataac ttcttctacc gggatttcta cacccaggtc
    27781 gccacgcgca accatgatgc cgtcagaggc ttcgaggatt tcgtcgaagt tgttgaggcc
    27841 ttcctggttt tcgattttgg agatgatgtg gatgttttcg ccgccgtgcg ctttcaggtg
    27901 ctcacggatt tcgataacgt cagaacgctt acgaataaag gaagcagcaa caaagtctac
    27961 gccttgttcg caaccaaaga tcaggtcctg tttgtctttt tcagccagtg ctggcagggc
    28021 aatggaaacg ccaggcaggt tcacaccttt gttttcgccc aggtcgccgt tgttcagcac
    28081 tttacagata actttgttac cttcaatggc ggtaacttcc ataccgatca gaccgtcgtc
    28141 aaccagtacg gtgttgccaa cagacaggtc agtagtgaaa ccttcatacg ttaccgcaac
    28201 catttcgctg ttgccgataa cagatttatc agtagtgaaa gtaaaggact gacccgcttt
    28261 cagagaaacg tcgttaccgc cttccagttt catggtgcgg atttctggac ctttggtatc
    28321 aagcaggatg gcggcggttt taccagtttt gctcatcacg ttgcgcagat tctgaatgcg
    28381 ctgaccgtgt tctgcatagt caccatgaga gaagttcaga cgcataacgt tcatgccagc
    28441 gtccagcatt ttagctaaca tctcttcaga ttcggttttc ggtccgatgg tgcaaacaat
    28501 tttggtcttt ttcatgacag tcttagtctt taagttgaga aggatgggag aaacttgctt
    28561 tctgggcgca ctgccggaaa gcgaatccgg tattacgaaa gtggttgtgc ctcgctctaa
    28621 ggataggtga catcgaaaaa gcgtgcagag gaatgtgtgc ttgtgtctca gcccaacgtg
    28681 agtgcctgtt tttgagtcgt tgagttctac agtccaaagt gctgaaacca ttcaagagtc
    28741 aattggcgcg cattatacgc tgaaattaat ccaaaaggaa aacaaaaaca gcgtttcaaa
    28801 aaatatacaa cttgtaagac aataaattgt tatcaaagat tcagcgttcc agggaaaagg
    28861 ttacgcaaaa acttcgttgt ttgcgcaacg tctggtgccg gggttgtcag tgtgaggata
    28921 agtgaaaagt tcatccggtg tataaataca tcatcgctca attgctacag ttaaaataat
    28981 ttatcacgtt atttgtctga ttttgtttga tttttgttta attggttaga ttgatatatg
    29041 ctgatagttg gcatgataaa taggtataaa gaatgggaaa ccggacaaaa gaggatgaac
    29101 tgtaccgaga aatgtgcaga gttgtcggta aagttgtgct ggaaatgcgc gatctggggc
    29161 aggaaccaaa gcatattgtt atcgcaggcg tattacgtac agcattagcc aataaacgca
    29221 ttcagcgcag tgagctggaa aagcaggcga tggaaacagt cattaacgca ctggtaaaat
    29281 gaccattcag gatgaggcgg taagcttcat cctgatattg ttagttcagt tgcggtccag
    29341 ttaatcattc taaataacaa ctg
//
LOCUS       DAZGJT010000055        28983 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZGJT010000055 DAZGJT010000000
VERSION     DAZGJT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28983
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.055"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            582..1721
                     /gene="wza"
                     /locus_tag="ACHKFK_002683"
     CDS             582..1721
                     /gene="wza"
                     /locus_tag="ACHKFK_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO9052984.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGESGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1727..2170
                     /gene="wzb"
                     /locus_tag="ACHKFK_002684"
     CDS             1727..2170
                     /gene="wzb"
                     /locus_tag="ACHKFK_002684"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO9052985.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFTAVYTLLERSARQWAQALNAEQV"
     gene            2173..4335
                     /gene="wzc"
                     /locus_tag="ACHKFK_002685"
     CDS             2173..4335
                     /gene="wzc"
                     /locus_tag="ACHKFK_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO9052986.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTIIEARWWVIGITAVFALC
                     SVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADRVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRNRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVKWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4428..5267
                     /gene="wcaA"
                     /locus_tag="ACHKFK_002686"
     CDS             4428..5267
                     /gene="wcaA"
                     /locus_tag="ACHKFK_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO9052987.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYNNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWIPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5270..5742
                     /gene="wcaB"
                     /locus_tag="ACHKFK_002687"
                     /pseudo
     CDS             5270..5742
                     /gene="wcaB"
                     /locus_tag="ACHKFK_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            5739..6956
                     /gene="wcaC"
                     /locus_tag="ACHKFK_002688"
     CDS             5739..6956
                     /gene="wcaC"
                     /locus_tag="ACHKFK_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO9052988.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVCETQGKSKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6931..8148
                     /gene="wcaD"
                     /locus_tag="ACHKFK_002689"
     CDS             6931..8148
                     /gene="wcaD"
                     /locus_tag="ACHKFK_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO9052989.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVVLAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNEEAIKTKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8159..8905
                     /gene="wcaE"
                     /locus_tag="ACHKFK_002690"
     CDS             8159..8905
                     /gene="wcaE"
                     /locus_tag="ACHKFK_002690"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO9052990.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQADDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKVQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYRAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8921..9469
                     /gene="wcaF"
                     /locus_tag="ACHKFK_002691"
     CDS             8921..9469
                     /gene="wcaF"
                     /locus_tag="ACHKFK_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO9052991.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGANSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVMIGNGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            9496..10617
                     /gene="gmd"
                     /locus_tag="ACHKFK_002692"
     CDS             9496..10617
                     /gene="gmd"
                     /locus_tag="ACHKFK_002692"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO9052992.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10620..11585
                     /gene="fcl"
                     /locus_tag="ACHKFK_002693"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10620..11585
                     /gene="fcl"
                     /locus_tag="ACHKFK_002693"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO9052993.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIQAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMVESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWQENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11588..12067
                     /gene="gmm"
                     /locus_tag="ACHKFK_002694"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11588..12067
                     /gene="gmm"
                     /locus_tag="ACHKFK_002694"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO9052994.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTSDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            12064..13287
                     /gene="wcaI"
                     /locus_tag="ACHKFK_002695"
     CDS             12064..13287
                     /gene="wcaI"
                     /locus_tag="ACHKFK_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO9052995.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQAFLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13290..14726
                     /gene="cpsB"
                     /locus_tag="ACHKFK_002696"
     CDS             13290..14726
                     /gene="cpsB"
                     /locus_tag="ACHKFK_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO9052996.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14919..16289
                     /gene="cpsG"
                     /locus_tag="ACHKFK_002697"
                     /gene_synonym="manB"
     CDS             14919..16289
                     /gene="cpsG"
                     /locus_tag="ACHKFK_002697"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO9052997.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEGNDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMSAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16344..17738
                     /gene="wcaJ"
                     /locus_tag="ACHKFK_002698"
     CDS             16344..17738
                     /gene="wcaJ"
                     /locus_tag="ACHKFK_002698"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO9052998.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGGRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17740..19218
                     /gene="wzxC"
                     /locus_tag="ACHKFK_002699"
     CDS             17740..19218
                     /gene="wzxC"
                     /locus_tag="ACHKFK_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO9052999.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVVVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSSALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRVG"
     gene            19494..20774
                     /gene="wcaK"
                     /locus_tag="ACHKFK_002700"
     CDS             19494..20774
                     /gene="wcaK"
                     /locus_tag="ACHKFK_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO9053000.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACKLTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20771..21991
                     /gene="wcaL"
                     /locus_tag="ACHKFK_002701"
     CDS             20771..21991
                     /gene="wcaL"
                     /locus_tag="ACHKFK_002701"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO9053001.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLHHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            22002..23396
                     /gene="wcaM"
                     /locus_tag="ACHKFK_002702"
     CDS             22002..23396
                     /gene="wcaM"
                     /locus_tag="ACHKFK_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO9053002.1"
                     /translation="MPFKKLSRRTFLAASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINTGIFIPAGKTLHILGSLRGNGRGRFILQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     NHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVISNIK
                     ARNITPDFSKKAGIDNAAVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23554..24549
                     /locus_tag="ACHKFK_002703"
     CDS             23554..24549
                     /locus_tag="ACHKFK_002703"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HFO9053003.1"
                     /translation="MNDNVLLIGASGFVGTRLLETAIADFNIKNLDKQQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            24791..25684
                     /gene="galF"
                     /locus_tag="ACHKFK_002704"
     CDS             24791..25684
                     /gene="galF"
                     /locus_tag="ACHKFK_002704"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO9053004.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKISRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            26056..27141
                     /gene="rfbB"
                     /locus_tag="ACHKFK_002705"
     CDS             26056..27141
                     /gene="rfbB"
                     /locus_tag="ACHKFK_002705"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO9053005.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFHFHHISTDEVYGDLPHPDEVNNKEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNENKNIDVVLTICDL
                     LDEIVPKEQSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTVEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRK"
     gene            27141..28040
                     /gene="rfbD"
                     /locus_tag="ACHKFK_002706"
     CDS             27141..28040
                     /gene="rfbD"
                     /locus_tag="ACHKFK_002706"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFO9053006.1"
                     /translation="MNILLFGKTGQVGWELQRSLAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKAANEVGAWVIHF
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRMALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLKAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            28098..28976
                     /gene="rfbA"
                     /locus_tag="ACHKFK_002707"
     CDS             28098..28976
                     /gene="rfbA"
                     /locus_tag="ACHKFK_002707"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO9053007.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGAHQSLIEASNFIATIEERQGLKVSCPEEIAFRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGD"
BASE COUNT         7302 a         6972 c         7747 g         6962 t
ORIGIN      
        1 tgcagcacta tttatctcat attatttgag ggttataacc tcacattatc cccgaattat
       61 aagtgataat aataaagcgt tatttaagaa atatcgcatg tggagacgcc atattaaata
      121 ttgtcaacct aaagaaactc ctaaaaacca tattgaatga cacttaatat aattcttaaa
      181 aatagccaat tgccgaattg ttaccttgcc tgctattccg ttagctgtaa cacttccccc
      241 tgcattattg gagagccaat attcaattac cataatgtca ccttatattc ggaaattaat
      301 ttccgcaata accctttctc atcaacgact gtatgcagag agggtttgaa aaattaaact
      361 aaatcagtgt attggtagct aaaaagccag gggcggtagc gtgtctggat gcctgaaaga
      421 ccagtctgaa ttattctgtc aatagcctgc ggatgaaatc aattttttta ggactgatgc
      481 cagttaaatt tttattcaat taactgcaag gtaaatgtgc atggcgacat tgctattaat
      541 agtgcacagg ataattactc tgccaaagtg ataaataaac aatgatgaaa tccaaaatga
      601 aattgatgcc attattggta tcagtaacct tgataagcgg ttgcacagta cttccgggca
      661 gcaatatgtc gacgatgggg aaagacgtca tcaaacagca ggacgctgat ttcgacctcg
      721 acaaaatggt gaatgtttat ccgttaaccc cgcgcctgat tgatcaatta cgcccacgac
      781 ccaacgtagc gcgccccaat atgacgctgg aaagtgagat cgcgaattac cagtatcgcg
      841 tcgggccggg ggacgttctt aatgtcaccg tctgggatca cccggaactc actacaccag
      901 ccggtcagta ccgcagctcc agcgacaccg gcaactgggt acagcctgac ggcaccatgt
      961 tctacccgta tatcggcaag gttcacgtcg tggggaaaac gctcgctgaa atccgcagtg
     1021 atattaccgg gcgcttagcg acgtatatcg ctgacccgca ggtggacgtt aatatcgccg
     1081 ccttccgctc gcaaaaagcc tatatctccg gtcaggtgaa taaatccggt caacaggcga
     1141 ttaccaacgt gccactgacc attctcgacg ccatcaacgc cgcaggtggc ctgaccgaca
     1201 ccgctgactg gcgcaacgtg gtgctaacac acaatggtcg tgaagagcgc atttctttgc
     1261 aggcgctgat gcaaaacggc gacctcaatc agaaccgact gctttacccc ggcgatattc
     1321 tttacgtgcc acgtaatgat gatctgaaag tgtttgtgat gggtgaagtg aagaaacaga
     1381 gcaccctgaa aatggacttt agcggcatga ccctgactga agccctgggc aatgctgaag
     1441 gcatcgacat gaccacctcc aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg
     1501 agagcgggcg taacggcaag attgccaata tctaccagct ggatatgtcc gatgcgacgt
     1561 cgctggtaat ggcgacagaa ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg
     1621 ccccggtatc ccggtggaac cgtctgatca atcagttgct gccaactatt agcggtgtcc
     1681 gttacatgac ggatacagcc agcgacattc ataactggta atcgtcatgt ttaacaacat
     1741 cttagttgtc tgtgtcggca atatttgccg ttccccgacg gcggaacgct tactgcaacg
     1801 ttatcacccg gagctaaaag tggagtccgc tggactcggc gcgctggtcg gtaagggcgc
     1861 tgatcctacc gctatcagcg tcgccgcaga acatcaactg tccctggaag gtcattgcgc
     1921 acggcaaatc agccgccgtc tgtgtcgcaa ctacgacctg attctgacca tggaaaagcg
     1981 ccatatcgaa cgcttatgcg agatggcacc agagatgcgc ggcaaagtga tgctgtttgg
     2041 tcactgggat aacgaatgtg aaatccccga tccgtatcgc aaaagccggg aaacgtttac
     2101 agcggtgtac acattacttg aacggtctgc ccgccagtgg gcgcaggcat tgaacgcaga
     2161 gcaggtataa gaatgacaga aaaagtaaaa caacatgccg ctccggtaac gggcagtgat
     2221 gaaatcgata ttggtcgcct ggtcggcacc atcattgaag cacgctggtg ggtgattggc
     2281 atcaccgctg tatttgccct ctgttccgtg gtttacactt tcttcgccac gccgatttat
     2341 agtgccgacg cactggtaca aatcgagcaa aacagtggca attcgttagt gcaggacatt
     2401 ggttcggcat tagccaacaa accgccagca tcggacgccg agatccagtt gattcgttcg
     2461 cgtctggtgc ttggtaaaac ggtggacgat ctcgacctcg atattgcggt gagcaaaaac
     2521 acgttcccga ttttcggtgc gggctgggat cgcctgatgg gacgtcagaa cgagacggtg
     2581 aaagtgacca cctttaaccg cccgaaagag atggcggatc gggtgtttac gcttaatgtg
     2641 cttgatgaca aaaactacac cctgagcagt gatggcggct ttagcgcccg tgggcaagcg
     2701 ggccagatgc tgaaaaaaga aggcgtcacg ctgatggttg aagctattca cgccagcccg
     2761 gggagtgagt ttaccgtcac caaatactcc acgctgggga tgatcaacca actgcaaaac
     2821 agcctgacgg taacggagaa cggaaaagac gcaggcgttt tgagcctgac ttataccggt
     2881 gaagatcgcg aacagatccg cgacattctt aacagcatcg cccgtaacta tcaggaacaa
     2941 aatattgagc gcaaatcggc ggaagcgtcg aaaagcctcg ctttcctcgc gcaacagtta
     3001 ccggaagtac gtaaccgcct tgatgttgcc gaaaacaaac tgaatgcctt ccgtcaggat
     3061 aaagattcag ttgatctgcc gctggaagcg aaagcggtgc tcgattcgat ggtgaacatc
     3121 gacgcgcagt tgaacgaact gacctttaaa gaggcggaaa tctccaagct gtacaccaaa
     3181 gttcacccgg cgtaccgcac gctgctggag aaacggcagg cgctggaaga cgaaaaagcc
     3241 aaacttaatg gtcgcgtaac ggcgatgccg aaaacccagc aggaaattgt gcgtctgacc
     3301 cgcgatgtcg agtctggtca gcaggtctat atgcaactgc tgaataaaga gcaggagctg
     3361 aaaatcaccg aagccagcac cgtcggcgat gtgcgcattg ttgacccggc aatcacccag
     3421 ccaggtgtac tgaaaccgaa gaaagggctg attatccttg gggcgattat ccttggcctg
     3481 atgctctcta tcgtgggtgt gctgctgcgc tcgttgttta atcgcggtat cgaaagcccg
     3541 caggtgctgg aagaacacgg tatcagcgtt tatgccagca tcccgctgtc ggagtggcag
     3601 aaagcgcgcg atagcgtcaa aaccatcaaa gggattaaac gctataaaca gagccagcta
     3661 ctggcggtgg ggaatccaac cgatctggcg attgaagcca tccgtagtct gcgtaccagt
     3721 ctgcacttcg cgatgatgca ggcacagaac aatgtgttga tgatgaccgg ggttagcccg
     3781 tcaatcggta aaacctttgt ctgcgccaac ctggcggcgg tgattagcca gaccaataaa
     3841 cgggtgttgt tgatcgactg cgatatgcgc aaaggctaca cccacgagct gttgggcact
     3901 aataacgtta atggcctgtc ggaaattctg attggacagg gcgatattac taccgctgct
     3961 aaaccgacct ctattgccaa atttgacctg atcccgcgcg gtcaggtacc gccaaatcct
     4021 tctgaactgt taatgagcga acgctttgcc gaactggtga agtgggcgag taaaaactac
     4081 gacctggtgt tgattgatac gccgccgatt ctggcagtga ccgatgcggc aattgttggt
     4141 cgtcatgtcg gaaccacgtt gatggtggcg cgttatgcag tcaacacatt gaaagaagtg
     4201 gaaaccagtc tgagccgctt tgagcaaaac ggtattccgg tgaaaggggt gattctgaac
     4261 tccatcttcc gccgcgccag cgcatatcag gattatggct attacgaata cgaatataag
     4321 tcggatgcga aataacgacg cctgcattcg caccgccccg taggccggat aaggcgttca
     4381 cgccgcatcc ggcaagcaaa ccagctcata agccgggaga acaacctatg aaagacaatc
     4441 cgctgatctc aatctatatg ccgacctgga atcgccaaca actggcgatt cgggcgataa
     4501 aatcggtcct gcgccaggac tataacaact gggagatgat catcgtggat gattgctcca
     4561 cctcctggga acaactccaa cagtacgtca ccgccctcaa cgatccgcgt atcacttaca
     4621 ttcacaacga cattaacagc ggggcgtgcg cggtacgtaa ccaggcgatt atgctggcgc
     4681 aaggggaata catcaccggg atcgatgacg acgatgaatg gatacccaac cgcctgagcg
     4741 tcttcctcgc ccataaacag caactggtca cacacgcctt tttgtacgct aacgactacg
     4801 tttgccaggg cgaagtttat tcccaaccgg caagcttgcc gctgtatccc aaatcgccgt
     4861 attcacgccg cctgttctac aaacgcaata tcattggcaa tcaggtcttt acctgggcgt
     4921 ggcgtttcaa agagtgcttg ttcgataccg aactgaaagc ggcacaggat tacgacatct
     4981 tcctgcggat ggtggtggag tacggcgaac cgtggaaagt ggaagaggcg acgcagatcc
     5041 tgcatatcaa tcacggcgaa atgcagatca cctcgtcacc gaaaaaattc tccggatact
     5101 tccactttta ccgcaagcac aaagacaaat tcgaccgcgc cagcaaaaaa tatcagctgt
     5161 ttaccctcta ccagatccgc aataaacgca tgacctggcg cactttgctg acgctgctgt
     5221 cggtgcgtaa cggcaaacgt ctggctgatg gtattcgggg gcgctaaaga tgctggaaga
     5281 tctgcgcgcc aacagctgga gtttacgccc gtgctgcatg gttctcgcct atcgcgtcgc
     5341 tcatttttgt tcggtgtggc gcaaaaagaa cgtcctcaac aatctgtggg cggccccgct
     5401 gctggtgctg tatcgcatta tcaccgaatg ctttttcggt tatgaaatcc aggccgccgc
     5461 tttactatcc atcacggtta cgccgtagtg atcaataaaa acgtggtagc gggggatgat
     5521 ttcaccattc gccacggcgt cactatcggc aatcgtggtg ccgataacat ggcatgtcca
     5581 cacattggca acggcgtcga actcggtgcc aacgtcatta ttcttggtga tatcacgctt
     5641 ggtaacaacg tcaccgtggg cgcgggcagc gtggtgctcg actctgtccc ggacaacgcg
     5701 ctggtggtgg gagaaaaagc gcgagtgaag gtaattaaat gaatattctg caatttaatg
     5761 tgcgactggc ggaaggcggg gcagcaggtg tggcgttaga tctccaccag cgcgcgctgc
     5821 aacaggggct ggcgtcacat tttgtctacg gctacggcaa aggcggcaaa gagagcgtca
     5881 gccatcagaa ctatccgcag gtcatcaaac atacgccacg gatgaccgca atggcgaaca
     5941 ttgcgttgtt tcgtctgttt aatcgcgatc tgttcggcaa tttcaatgag ttatatcgca
     6001 ccattactcg cacaccgggt ccggtggtcc tgcattttca tgtgctgcac agctactggt
     6061 tgaatcttaa gagcgtggtg cgcttttgtg aaaaagtgaa aaaccataaa ccggatgtca
     6121 ctctggtctg gacactgcac gaccactgga gcgttaccgg acgctgcgcc tttaccgacg
     6181 gttgcgaagg ctggaaaacg ggctgccaga aatgcccgac cttaaataat tatccgccgg
     6241 tgaagattga tcgcgcacac caactggtgg cgggcaaacg gcagttattt cgcgagatgc
     6301 tggcgctggg ctgtcagttt atttctccca gccagcatgt ggctgatgct ttcaatagtc
     6361 tgtacggtcc agggcgttgc cggattatca ataacggtat tgatatggca accgaagcga
     6421 ttctggcgga cttgcctccg gtgtgcgaaa cccagggcaa gtcgaaaatc gcggtggtgg
     6481 cgcatgacct gcgttacgac ggcaaaacta accagcaact ggtgcgcgag atgatggcgc
     6541 tgggtgacaa aatcgaactg catactttcg gtaagttctc gccgttcacc gctggtaacg
     6601 ttgttaatca cggctttgaa actgacaagc gcaagttgat gagcgcgctc aaccagatgg
     6661 atgcgctggt attcagttct cgcgtcgata actacccgct gattttgtgt gaggcgttat
     6721 cgattggcgt gccggtgatc gccacccata gcgatgcggc gcgggaagtg ctgcaaaaat
     6781 ccggtggtaa aaccgtcagc gaagaagatg tgctgcaact ggtgcagtta agcaaaccgg
     6841 aaatcgcaca ggcgatattt ggtaccacgc tggctgagtt cagccagcgc agccgcgccg
     6901 cctacagtgg acaacagatg ctggaggagt atgtcaactt ctatcagaat ctgtagctac
     6961 ctgctgctgc cgctgatcta cctgctggtt aacgtcaaaa tcgcccagct tggcgaaagt
     7021 ttccccatca ccatcgtcac ttttttacct gtcttgttac tgctgttttt agagcgcatc
     7081 agcgttaaaa aattgatgat tgccttaggg attggcgcgg ggctcacggc gtttaactac
     7141 ctgtttggtc agtcgttgga tgccagtaaa tatgtcactt caaccatgct gtttgtctat
     7201 attgtgatca ttattggcat ggtgtggagt attcgcttta aaacgatttc gccacacaac
     7261 catcgcaaga tattacgttt cttttatctg gtggtcgggc tggtggtggt gctggcggcg
     7321 gtggagatgg cgcaaattat cctcaccggc ggtagcagta ttatggagtc gatttcgaaa
     7381 tatctgattt acagcaacag ctatgtgctg aatttcatta aattcggcgg caagcgcacg
     7441 acagcacttt atttcgaacc ggcatttttc gctctggcat taatctcaat ttggctcagc
     7501 atcaaacagt ttggtatcaa aacgcctaaa accgatgcta tgattcttgc agggataata
     7561 ttatccggat cgttttcagg agttatgacc tttatccttt tttatttgct ggagtgggca
     7621 tttcaatatc tgaatgaaga ggcgattaag acaaagttac cgttagcatt gatttctctg
     7681 gctgtattcc tggttggtgt ggtaatcgcg ttcccttata tttccacccg tctgggcgat
     7741 ttaggtacag aaggatcgtc atcgtattat cgtattgtcg gtccgctggt gatggtcggt
     7801 tattctttaa cccatattga cggtgtagtc agatttggct cactttatga atatgtcgca
     7861 tcattcggaa tatttaacgg tgcggatgtc ggaaaaacca tagacaatgg tttgtatctg
     7921 ctgattattt atttttcctg gttcgcggtg tttttatcac tgtggtacat ggggaaagtg
     7981 ataaaaatga tgatcaacgc ttttggtgat aaccgcaatt ttcgcgtgca attatatctt
     8041 tttactccgg tatcgctgtt ttttaccggt tcgatattta gcccggaata tgcattttta
     8101 atcgtctgtc cgtttatttt gcgaaaagcg ttaaatatta cgaggtaaga ataagaacat
     8161 gttgcttagc ataatcactg tcgcgtttcg taacctcgaa gggatagtca aaacacatgc
     8221 ctcgctggcg catctggcgc aggcggacga tatcagcttc gaatggattg ttgtcgatgg
     8281 cggttccaat gacggcaccc gtgagtatct ggaaaatctc aatggtatct ataacctacg
     8341 ctttgtcagc gagccagata acggtatcta cgacgctatg aacaaaggca ttgcgatggc
     8401 gcaaggcaag ttcgcgttgt ttctcaactc aggcgatatt tttcatcagg atgccgcaaa
     8461 ttttgtccgt aagttaaaag tgcaaaaaga taacatgatg atcaccggcg atgcgctgct
     8521 ggattttggc gacgggcata aaattaaacg tagcgccaaa ccgggttggt atatttacca
     8581 cagtctgccc gccagtcatc aggcgatatt tttcccggtg tccggcttga agaaatggcg
     8641 ttatgatctg gaatataaag tttcttccga ctacgcgctg gcggccaaaa tgtatagagc
     8701 cggttatgca tttaaaaaac tcaatggcct ggtgtctgaa ttttctatgg gtggggtatc
     8761 aaccaccaat aatatggaat tgtgtgctga cgcaaaaaaa gtccaacggc agatattgca
     8821 cgtgcctggc ttttgggctg aattatcctg gcatttacgc caacgtacga cctcaaagac
     8881 gaaagcctta tataacaaaa gctgaatata agggaaaacg atgcaggatt taagtggatt
     8941 ctcggtgccg aaagggttcc ggggtggcaa cgctattaaa gtgcaattat ggtgggcagt
     9001 acaggcaaca atatttgcct ggtcgccaca agtattgtat cgctggcggg catttttatt
     9061 acgtttattc ggcgcaaaaa taggaaaaaa cgtagtgatt cgtccgtcag taaaaattac
     9121 ctatccgtgg aaattaacct taggtgatta cgcgtgggtc ggcgatgacg tcaatttata
     9181 taccctcggt gaaataacca ttggcgcaaa ttcggtgata tcgcaaaaaa gttatttatg
     9241 caccggtagc cacgaccatg caagtcaaca tttcaccatt aacgccacgc ctattgtgat
     9301 tggcgagaaa tgctggctgg caaccgatgt ctttgttgcc ccaggcgtca tgatcggcaa
     9361 cggcaccgtc gtgggtgcgc gaagcagtgt ttttaaatcg cttccggcaa atgtggtttg
     9421 ccgggggaat cccgcagtgg tgatacgcga acgcgttgaa actgaataaa ttcaaaaaat
     9481 acagaggaat aagacatgtc aaaagtcgct ctcatcaccg gtgtaaccgg acaagacggt
     9541 tcttacctgg cagagtttct gctggaaaaa ggttacgagg tgcatggtat taagcgtcgt
     9601 gcatcgtcat tcaacaccga gcgcgtggat cacatttatc aggatccgca cacctgcaac
     9661 ccgaaattcc atctgcatta tggcgacctg agtgatacct ccaacctgac gcgcattttg
     9721 cgtgaagtgc agccggatga agtgtacaac ctgggcgcaa tgagccacgt tgcggtttct
     9781 tttgagtcac cggaatatac cgcagacgtt gatgcgatgg gtacgctgcg tctgctggag
     9841 gcgatccgct tcctcggtct ggaaaagaaa acccgtttct atcaggcttc cacctctgaa
     9901 ctgtatggtc tggtgcagga aattccgcag aaagaaacca cgccgttcta tccacgctct
     9961 ccgtatgcgg tcgccaaact gtacgcctac tggatcaccg ttaactaccg cgaatcctac
    10021 ggcatgtacg cctgcaacgg tattctcttt aaccatgaat cccctcgccg tggcgaaacc
    10081 ttcgtcactc gcaaaatcac ccgcgcaatc gccaatatcg cccaggggct ggagtcgtgc
    10141 ctgtatctcg gcaatatgga ttccctgcgt gactggggcc acgccaaaga ctacgtaaaa
    10201 atgcagtgga tgatgctgca acaggaacaa ccggaagatt tcgttattgc caccggcgtt
    10261 cagtactccg tgcgtcagtt cgtggaaatg gcggcggcac agctgggcat caaactgcgc
    10321 tttgaaggca cgggcgttga agagaagggc attgtggttt ccgtcaccgg gcatgacgcg
    10381 ccgggcgtta aaccgggtga tgtgattatc gccgttgacc cgcgctactt ccgtccggct
    10441 gaagttgaaa cgctgctcgg cgacccgacc aaagcgcacg aaaaactggg ctggaaaccg
    10501 gaaatcaccc tcagagagat ggtgtctgaa atggtggcta atgaccttga agcggcgaaa
    10561 aaacactctc tgctgaaatc tcacggctac gacgtggcga tcgcgctgga gtcataagca
    10621 tgagtaaaca acgcattttt attgctggcc atcgcgggat ggtcggttcc gccatcaggc
    10681 ggcagctcga acagcgcggt gatgtagagc tggtattacg cacccgcgat gaactgaacc
    10741 tgctggacag ccgcgccgtg catgatttct ttgccagcga acgtattgac caggtctatc
    10801 tggcggcggc gaaagtgggc ggcattgttg ccaacaacac gtatccggcg gatttcatct
    10861 atcagaacat gatgattgag agcaatatca ttcaagccgc gcatcagaac gacgtgaaca
    10921 aactgctgtt tctcggatcg tcctgcatct acccgaaact ggcaaaacag ccgatggtag
    10981 aaagcgagtt gttgcagggc acgctggagc cgaccaacga gccttatgcc attgccaaaa
    11041 tcgccgggat caaactgtgt gaatcttaca accgccagta cggacgggat taccgctcag
    11101 tcatgccgac caacctgtac gggccgcacg acaacttcca tccgagtaat tcgcatgtga
    11161 tccctgcatt gctgcgtcgc ttccacgagg cgacggcaca gaatgcgccg gacgtggtgg
    11221 tgtggggcag cggtacgccg atgcgcgaat ttctgcacgt cgatgatatg gcggcggcaa
    11281 gcattcatgt gatggagctg gcgcatgaag tctggcagga gaacacccag ccgatgctgt
    11341 cgcacattaa cgtcggcacg ggcgttgact gcactatccg cgagctggcg caaaccatcg
    11401 ccaaagtggt gggttacaaa ggtcgggtgg tttttgatgc cagcaaaccg gatggcacgc
    11461 cgcgcaaact gctggatgtg acgcggctgc atcagcttgg ctggtatcac gaaatctcac
    11521 tggaagcggg gcttgccagc acttaccagt ggttccttga gaatcaagac cgctttcggg
    11581 ggtaatgatg tttttacgtc aggaagactt tgccacggta gtgcgctcca ctccgcttgt
    11641 ctctctcgac tttattgtcg agaacagtcg cggcgagttt ctgcttggca aaagaaccaa
    11701 ccgcccggcg cagggttact ggtttgtgcc gggagggcgc gtgcagaaag acgaaacgct
    11761 ggaagccgca tttgagcggc tgacgatggc ggaactgggg ctgcgtctgc cgataacagc
    11821 aggccagttt tacggtgtct ggcagcactt ttatgacgat aacttctctg gcacggattt
    11881 caccactcac tatgtggtgc tcggttttcg cttcagagta gcggaagaag aactgttact
    11941 gccggatgag cagcatgacg attaccgctg gctgacgtcg gacgcactgc tcgccagtga
    12001 caatgttcac gctaacagcc gcgcctattt tctcgctgag aagcgtgccg gagtacccgg
    12061 attatgaaaa tactggtcta cggcattaac tactcgccgg agttaaccgg catcggcaag
    12121 tacaccggcg agatggtgga atggctggca gcacaaggtc atgaggtgcg ggttattacc
    12181 gcgccgcctt actacccgca gtggcaggtg ggcgagaact attccgcctg gcggtacaaa
    12241 cgggaagagg gggccgccac ggtgtggcgc tgcccgctgt acgtgccaaa acagccgagc
    12301 accctgaaac gcctgttgca tctcggcagt tttgccgtca gcagtttttt cccgttgatg
    12361 gcgcaacgtc gctggaagcc ggatcgcatt attggcgtgg tgccaacgct gttttgcacg
    12421 ccgggaatgc gcctgctggc gaaactctct ggtgcgcgta ccgtgctgca tattcaggat
    12481 tacgaagtgg acgccatgct ggggctgggc cttgccggaa aaggtaaagg cggcaaagtg
    12541 gcacagctgg caacggcgtt cgaacgtagc ggactgcata acgtcgataa cgtctccacg
    12601 atttcgcgtt cgatgatgaa taaagctatc gagaaaggcg tggcggcgga aaacgtcatc
    12661 ttcttcccca actggtcaga aattgcccgt tttcagcatg tcgcagacgc cgatgttgat
    12721 gcccttcgta accagcttgg cctgccggat aacaaaaaaa tcattcttta ctccggcaat
    12781 attggtgaaa agcaggggct ggaaaacgtt attgaagctg ccgatcgcct gcgcgatgaa
    12841 ccgcttattt ttgccattgt cgggcagggc ggcggcaaag cgcggctgga aaaaatggcg
    12901 cagcagcgtg gactgcgcaa catgcaattt tttccgctgc aatcgtatga cgctttaccc
    12961 gcactgctga agatggggga ttgccatctg gtggtgcaaa aacgcggcgc ggcagatgcc
    13021 gtattgccgt cgaaactgac caatattctg gcggtaggcg gtaacgcggt gattaccgca
    13081 gaagcccaca cagaactggg gcagctttgc gaaacctttc cgggcattgc ggtttgcgtg
    13141 gaaccggaat cggtcgaggc gctggtggcg gggattcgtc aggcgttcct gctgcccaaa
    13201 cacaacacgg tggcacgtga atatgccgaa cgcacgctcg ataaagagaa cgtgttacgt
    13261 caatttataa atgatattcg gggataatta tggcgcagtc gaaactctat ccagttgtga
    13321 tggcaggtgg ctccggtagc cgcttatggc cgctttcccg cgtactttac cccaagcagt
    13381 ttttatgcct gaaaggcgat ctcaccatgc tgcaaaccac catctgccgc ctgaacggcg
    13441 tggagtgcga aagcccggtg gtgatttgca atgagcagca ccgctttatt gtcgcggaac
    13501 agctgcgtca actgaacaaa ctcaccgaga acattattct cgaaccggca gggcgtaaca
    13561 ctgcaccggc cattgcgctg gcggcgctgg cggcaaaacg tcatagcccg gagaacgacc
    13621 cattaatgct ggtgctggcg gcggatcatg tgattgccga tgaagacgcg ttccgtgccg
    13681 ccgtgcgtaa tgccatgccg tatgccgaag cgggcaagct ggtgaccttc ggcattgtgc
    13741 cggatctacc tgaaaccgga tatggctata ttcgtcgcgg tgaagtgtcg gcgggtgagc
    13801 aggatacggt ggcctttgaa gtggcgcagt ttgtcgaaaa accgaatctg gaaaccgcgc
    13861 aggcctatgt ggcaagtggc gaatattact ggaacagcgg tatgttcctg ttccgtgccg
    13921 gacgttatct cgaagaactg aaaaagtatc gtccggatat tctcgatgcc tgtgaaaaag
    13981 cgatgagcgc cgtcgatccg gatctcgatt ttattcgtgt ggatgaagag gcgtttctcg
    14041 cctgtccgga agagtcggta gattacgcgg tcatggaacg cacggcagat gccgttgtgg
    14101 tgccgatgga tgcgggctgg agcgatgtcg gttcctggtc ctctttatgg gagatcagtg
    14161 cccacaccgc cgagggcaac gtttgccatg gcgatgtgat taatcacaaa actgaaaaca
    14221 gctatgtgta tgccgaatct ggcctggtca ccaccgtcgg ggtgaaagat ttggtggtag
    14281 tgcagaccaa agatgcagtg ctgattgccg accgtaacgc ggtgcaggat gtgaaaaaag
    14341 tggtcgagca gatcaaagct gatggtcgcc atgagcatcg ggtgcatcgc gaagtgtatc
    14401 gtccgtgggg caaatatgac tctatcgacg cgggcgaccg ctaccaggtg aaacgcatca
    14461 ccgtgaagcc gggcgaaggg ttgtcggtac agatgcatca tcaccgcgcg gaacactggg
    14521 tggttgtcgc gggaacggca aaagtcacta tcgacggtga tatcaaactg cttggtgaaa
    14581 acgagtccat ttatattccg ctgggggcga cgcattgcct ggaaaacccg gggaaaattc
    14641 cgctcgattt aattgaagtg cgctccggct cttatctcga agaggatgat gtggtgcgct
    14701 tcgcggatcg ctacggacgg gtgtaaacgt cgcatcaggc gctgtttgtc ggatgcggcg
    14761 tgaacgcctt atccgaccta cggttcgggt ttgtaggcct gataagacgc ggcagcgtcg
    14821 catcaggcaa tgaatgcgaa accgcgatgt aaataacgac aaaaacaaaa ttggccgttt
    14881 cggtcagggc caactattgc ctgaaaaagg gtaacgatat gaaaaaatta acctgcttta
    14941 aagcctatga tattcgcgga aaattaggcg aagaactgaa tgaagatatc gcctggcgca
    15001 ttggtcgcgc ctatggcgaa tttctcaaac cgaaaaccat tgtgttaggc ggtgatgtcc
    15061 gcctcaccag cgaaacctta aaactggcgc tggcgaaagg tttacaggat gcgggcgtcg
    15121 acgtgctaga tattggtatg tccggcaccg aagaaatcta tttcgccacg ttccatctcg
    15181 gcgtggatgg cggcattgaa gttaccgcca gccataaccc gatggattac aacggcatga
    15241 aactggtgcg cgaaggggct cgcccgatca gcggcgatac cggactgcgc gacgtccagc
    15301 gtctggcaga aggcaacgac tttcctcccg tcgatgaaac caaacgcggt cgctatcagc
    15361 aaatcaatct gcgtgacgct tacgttgatc acctgttcgg ttatatcaac gtcaaaaacc
    15421 ttacgccgct caagctggtg attaactcag ggaacggcgc ggcgggtccg gtggtggacg
    15481 ccattgaagc ccgctttaaa gccctcggcg caccggtgga attgatcaaa gtgcataaca
    15541 cgccggacgg caatttcccc aacggtattc ctaacccgtt gctgccggaa tgtcgcgacg
    15601 atacccgcaa tgcggtcatt aaacacggcg cggatatggg cattgccttt gatggcgatt
    15661 ttgaccgctg tttcctgttt gacgaaaaag ggcagtttat tgagggctac tacattgtcg
    15721 gcctgctggc agaagcgttc ctcgaaaaaa atcccggcgc gaagatcatc cacgatccac
    15781 gtctctcctg gaacaccatt gatgtggtga cggccgcagg cggcaccccg gtaatgtcga
    15841 aaaccggaca cgcctttatt aaagaacgta tgcgcaagga agacgctatc tacggtggcg
    15901 aaatgagcgc ccaccattac ttccgtgatt tcgcttactg cgacagcggc atgatcccgt
    15961 ggctgctggt cgccgaactg gtgtgcctga aaggaaaaac gctgggcgaa ctggtgcgcg
    16021 accggatgtc agcgtttccg gcaagcggtg agatcaacag caaactggca caacctgttg
    16081 aggcgattaa ccgcgtggaa cagcacttta gccgcgaggc gctggcggtg gatcgtaccg
    16141 atggcatcag catgaccttt gccgactggc gctttaatct gcgcacctcc aacaccgaac
    16201 cggtggtgcg gttgaatgtg gaatcacgcg gtgatgtgcc gctgatggaa gcgcgaacgc
    16261 gaactctgct gacgttgctg aacgagtaat gtcggatctt cccttacccc actgcgggta
    16321 aggagctaaa aacaggaaca acgatgacaa atctaaaaaa gcgcgagcga gcgaaaacca
    16381 atgcatcgtt aatctctatg gtgcaacgct tttcagatat caccatcatg tttgccgggc
    16441 tatggctggt ttgcgaagtg agcggactgt cattcctcta catgcacctg ttggtggcgc
    16501 tgattacgct ggtggtgttc cagatgctgg gcggcatcac cgatttttat cgctcatggc
    16561 gcggtgttcg ggcagcgaca gaatttgccc tgttgctaca aaactggacg ttaagcgtga
    16621 ttttcagcgc cggactggtg gcgttcaaca atgatttcga cacgcaattg aaaatctggc
    16681 tggcgtggta tgggctgacc agcatcggac tggtggtttg ccgttcatgt attcgtattg
    16741 gggcgggctg gctgcgtaat catggctata acaagcgcat ggtcgcggtg gcgggggatt
    16801 tagccgccgg acaaatgctg atggagagct tccgtaatca gccgtggtta gggtttgaag
    16861 tggtgggcgt ttaccacgac ccgaaaccgg gcggcgtttc taacgactgg gcgggtaacc
    16921 tgcaacagct ggtcgaggat gctaaagcgg gcaagattca taacgtctat atcgcgatgc
    16981 aaatgtgcga cggcgcgcga gtgaaaaaac tggtccatca attggcggac accacctgtt
    17041 cggtgctgct gatccccgat gtctttacct tcaacattct ccattcacgt ctcgaagaga
    17101 tgaacggcgt tccggtggtg ccgctttacg acacgccgct ttccggggtt aaccgcctgc
    17161 tcaaacgtgc ggaagacatt gtgctggcga cgcttattct gttgctgatc tccccggtgc
    17221 tgtgctgtat tgcgttggcg gtgaaactca gttcaccagg gccggttatt ttccgccaga
    17281 ctcgctatgg catggatggc aaaccgatca aagtgtggaa gttccgttcg atgaaagtga
    17341 tggagaacga caaagtggtg acccaggcga cgcagaacga tccgcgcgtc accaaagtgg
    17401 ggaactttct gcgccgcacc tcgctggatg aactgccgca gtttatcaat gtgctgaccg
    17461 gggggatgtc gattgtcggt ccacgtccgc acgcagtagc gcataacgaa cagtatcgcc
    17521 agctcattga aggctacatg ctgcgccata aggtgaaacc gggcattacc ggctgggcgc
    17581 agattaatgg cgggcgcggc gaaaccgaca cgctggagaa aatggaaaaa cgcgtcgagt
    17641 tcgaccttga gtacatccgc gaatggagcg tctggttcga tatcaaaatc gttttcctga
    17701 cggtattcaa aggcttcgtt aacaaagcgg catattgata tgagcttacg tgaaaaaacc
    17761 atcagcggcg cgaagtggtc ggcgattgcc acggtgatca tcatcggcct cgggctggtg
    17821 cagatgaccg tgctggcgcg gattatcgac aaccaccagt tcggtttgct taccgtgtcg
    17881 ctggtgatca tcgcgctggc agatacgctt tctgacttcg gtattgctaa ctcgattatt
    17941 cagcgaaaag aaatcagcca ccttgaactc accacgctgt actggctgaa cgttgggctg
    18001 gggcttgtgg tgtgtgtggt ggtgtttttg ttgagtgatc tcatcggcga cgtgctgaat
    18061 aacccggacc tggcaccgtt gattaaaaca ttgtcgctgg cgtttgtggt gatcccccac
    18121 gggcaacagt tccgcgcgtt gatgcaaaaa gagctggagt tcaacaaaat cggcatgatc
    18181 gaaaccagcg cggtgctggc gggcttcact tttacagtgg ttagcgccca tttctggccg
    18241 ctggcgatga ccgcgatcct cggttatctg gtcaatagtg cggtgagaac gctgctgttt
    18301 ggctactttg gccggaaaat ttatcgcccc ggtctgcatt tctcgctggc gtctgttgcg
    18361 ccgaacttac gctttggtgc ctggctgacg gcggacagca tcatcaacta tctcaacacc
    18421 aacctttcaa cgctggtgct ggcgcgtatt ctcggcgcgg gcgtggcagg gggatacaac
    18481 ctggcgtaca acgtggccgt tgtgccgccg atgaagctga acccgatcat cacccgcgtg
    18541 ttgtttccgg cattcgccaa aattcaggac gataccgaaa agctgcgcgt taacttctac
    18601 aagctgttgt cggtagtggg gattatcaac tttccggcgc tactggggct gatggtggtg
    18661 tcgaataact ttgtaccgct ggtctttggt gagaagtgga acagcattat tccggtgctg
    18721 caattgctgt gtgtggtggg gctactgcgc tccgtgggta acccgattgg ttcgctgctg
    18781 atggcgaaag cacgggtgga catcagcttt aaattcaacg tattcaaaac atttctgttt
    18841 attccggcga ttgttattgg tgggcagatg gcgggcgcaa tcggcgtcac gcttggcttc
    18901 ctgctggtgc agattatcaa caccattctg agctatttcg tgatgattaa accggtgctc
    18961 ggttccagtt atcgccagta catcctgagt ttgtggctgc cgttttatct ctcgctgccg
    19021 acgctggtgg tcagttctgc gctgggcatt gtgctgaaag ggcaactggc gctggggatg
    19081 ctgctggcgg tgcaaatagc cgcgggtgtg ctggcgtttg tggtgatgat tgtgctgtcg
    19141 cgccatccgc tggtggtgga agtgaagcgt cagttttgtc gcagcgaaaa aatgaaaatg
    19201 cttttacggg tggggtgagt tgttgttttc ggttccgaca acggttgccg ggtgcagcgt
    19261 aaaacgcctt atccatccta cggttctgtg ctcgggacct gtaggcctga taagacgcgt
    19321 cagcgtcgca tcaggcactg aatgccggat gcggcgtgaa cgccttatcc ggcctacggt
    19381 tctgtgctcg ggtcttgtag gcctgataag acgcgtcagc gtcgcatcag gcaaaaagcg
    19441 gcaacgaaca aaacaaacac agcctcacct ggcatcaaat ttatgaggaa aaaatgaaat
    19501 tacttattct gggcaaccac acttgcggca atcgtggcga cagcgccatc ttgcgcggct
    19561 tacttgatgc catcaacatc ctcaatccac atgccgaagt ggacgtgatg agccgctatc
    19621 cggtcagttc ttcctggctg ctcaaccgcc cggtaatggg cgatccgctg ttcctgcaaa
    19681 tgaaacaaca caacagcgcg gcgggcgttg ttgggcgcgt taaaaaagtc ctccgtcgcc
    19741 gctaccagca tcaggtattg ctctcacgcg tcaccgacac tggcaagctg cgtaatatcg
    19801 ccatcgccca gggattcacc gacttcgtgc gcctgctgtc aggttacgac gccattattc
    19861 aggtcggcgg atcgtttttt gtcgatctct acggcgtgcc gcagtttgaa catgcgcttt
    19921 gcacgtttat ggcgaaaaag ccgctattta tgattggtca cagtgttggc ccgttccagg
    19981 atgagcaatt taaccaacta gcgaactatg tctttggtca ctgcgacgcg ctgatcctgc
    20041 gcgaatcggt cagccttgat ttgatgaaac gcagcaatat cactaccgca aaagtggaac
    20101 atggcgtcga taccgcgtgg ctggtcgatc accacacaga agacttcacc gccagctatg
    20161 ccgtccaaca ctggctggac gttgccgcac aacagaaaac ggtagcaatt accctgcgcg
    20221 aactggcacc gttcgacaaa cgtctcggca ccactcaaca agcgtatgaa aaagcctttg
    20281 ccggggtggt caatcgcatt ctcgacgaag ggtatcaggt gattgcgctt tccacctgta
    20341 cgggcatcga cagctataac aaagacgatc gcatggtggc gctcaatcta cgccagcaca
    20401 tcagcgatcc tgcccgttac cacgtagtga tggatgaact taacgatctg gaaatgggca
    20461 aaattctcgg agcctgtaaa ctcaccgtcg gtacgcgcct gcactccgcc attatctcaa
    20521 tgaactttgc cactccggcg attgccatca actatgaaca taaatccgcc gggattatgc
    20581 agcagctggg actgccagag atggcgattg atatccgtca tttactggac ggcagcctgc
    20641 aagcgatggt tgcggatacc ttaggccagc ttccggcgct gaacgcacga cttaacgaag
    20701 ctgtcagtcg tgagcgtcag acgggaatgc agatggtgca gtctgtgctt gagcgcatcg
    20761 gggaggtgaa atgaaggtcg gcttcttttt actgaaattt ccgttgtcgt cagaaacctt
    20821 cgtcctcaat caaattaccg cgtttattga tatgggattt gaggtggaga ttgtcgcgct
    20881 gcaaaaaggc gacacacaaa acacccacgc ggcatggacg aaatacaacc ttgccgccag
    20941 aacccgctgg ttacaggacg aacctacggg caaagtggcg aaactgcacc accgcgccag
    21001 ccagacctta cgcggcattc atcgtaaaaa tacctggcag gcgctcaacc tcaaacgcta
    21061 tggcgcagag tcgcggaacc tgattttgtc tgccatttgc ggtcaggtcg caacaccatt
    21121 tcatgccgat gtctttatcg ctcattttgg tccagcgggg gtaacagcag caaaactccg
    21181 cgaactgggt gtcattcgcg gcaaaattgc tactatcttc cacggcattg atatctcaag
    21241 tcgggaagtg ctcaaccact acactcccga atatcagcaa ctgttccgcc gtggcgacct
    21301 gatgctaccg ataagcgatc tgtgggccgg aaggctgcaa aaaatgggct gcccgaggga
    21361 aaaaatcgcc gtatcgcgca tgggcgtgga catgacgcgc tttagcccgc gtccggtgaa
    21421 agcgcctgca acgccgctgg aaatcatttc cgtcgcacgc ttaaccgaga aaaaaggcct
    21481 gcatgtggcg attgaagcct gtcggcagtt gaaagagcag ggcgtggcat ttcgctatcg
    21541 catcctcggc attggcccgt gggaacgacg cctgcgcacc ctcatcgaac aatatcaact
    21601 ggaagatgtg gtggagatgc cgggctttaa accgagccat gaagtgaaag cgatgctcga
    21661 cgacgcggat gtcttcctgt tgccatcggt aacgggtgcg gatggcgata tggaaggcat
    21721 tccggtagcg ctgatggaag cgatggcggt cggcattccg gtggtttcta ctctgcatag
    21781 cggaataccg gaattggtgg aggccgataa atccggctgg ctggtgcctg agaacgatgc
    21841 tcgcgcactg gcgcaacgac tggctgcgtt tagccaactg gacaccgacg aactggctcc
    21901 ggttgtcaaa cgtgcgcgcg aaaaagtcga acacgatttt aaccagcagg tgattaatcg
    21961 agaactcgcc agcttgctgc aggctttata gaggagaacg catgccattt aaaaaactct
    22021 cccgacgcac cttcctggcg gcaagctcgg cgcttgcctt cctccatacc cctttcgctc
    22081 gcgcacttcc cgcccgacaa agcgttaaca ttaacgacta caacccacac gactggatcg
    22141 cctcatttaa acaagccttc agcgaagggc aaacggtcgt cgtgcctgcc ggattggttt
    22201 gtgacaatat caacaccggc atcttcattc ctgcaggtaa aacgttacac atcttgggaa
    22261 gcctgcgcgg caacggcaga gggcgattta tcttacagga cggcagccag gtgacagggg
    22321 aggagggcgg cagtatgcat aacatcaccc tggatgtgcg tggctctgac tgcaccatca
    22381 aagggctggc gatgagcggc tttggcccgg taacgcagat ttatatcggc ggcaaaaaca
    22441 aacgggtcat gcgcaacctg accatcgata atctcaccgt taaccacgct aattacgcta
    22501 ttttgcgcca gggatttcat aaccagataa tcggtgccaa catcaccaat tgtaagttca
    22561 gtgacttaca aggcgacgcc attgaatgga acgtggcaat taacgacagt gatattttga
    22621 tatctgacca tgtcatcgag cgcatcaact gtactaacgg caaaatcaac tggggcattg
    22681 gcataggtct tgcgggaagt acctacgata acaactaccc ggaagaccag gcagtgaaaa
    22741 actttgtcgt ggcgaatatc acgggatcgg attgtcggca gttgatccat gttgaaaatg
    22801 gcaaacattt tgttattagt aatatcaaag cccgcaatat cacgccggat ttcagtaaga
    22861 aagcgggcat tgataacgcc gcggtcgcta tttacggttg tgacaatttc gtgattgata
    22921 atattgaaat gattaatagc gccgggatgt taatcggcta tggggtaatt aaaggcaaat
    22981 atctctcgat accgcaaaat ttccgagtga ataatattca actggataac acccatcttg
    23041 cttataaatt gcgcggcatc caaatctccg ccgggaatgc tgtctccttt gtggcgctga
    23101 ctaacattga gatgaagcgt gcgtcgctgg agttacacaa caaaccgcaa catcttttta
    23161 tgcgtaatat caaggtgatg caggaatcct cagttggacc agcattgagc atgaacttcg
    23221 acatgcgcaa agacgttcgc ggcgtcttta tggcgaaaaa agaaacactc ctgtctcttg
    23281 caaatgttca tgcggtgaat gaaagagggc aaagctccgt cgatatcgac aggataaatc
    23341 accatattgt taatgtggaa aagattaact ttagattgcc ggaacggagg gagtagattt
    23401 gcgaccattc ataaacttac tggatataat gttctaaaag attataatcc tcacactaac
    23461 tgttaaagct ggggctgggc agcattgact actcagggaa ggtactcatc tttatcttgt
    23521 gtttcttacg taacaaccag actaaagtga tttatgaacg ataacgtttt gctcatagga
    23581 gcttccggat tcgtaggaac ccgactactt gaaacggcaa ttgctgactt taatatcaag
    23641 aacctggaca aacagcagag ccacttttat ccagaaatca cacagattgg cgatgttcgt
    23701 gatcaacagg cactcgacca ggcgttagcc ggttttgaca ctgttgtgct actggcagcg
    23761 gaacaccgcg atgacgtcag ccctacttct ctctattatg atgtcaacgt tcagggtacc
    23821 cgcaatgtgc tggcggccat ggaaaaaaat ggcgttaaaa atatcatctt taccagttcc
    23881 gttgctgttt atggtttgaa caaacacaac cctgacgaaa accatccaca cgatcctttc
    23941 aaccactacg gcaaaagtaa gtggcaggca gaggaagtgc tgcgtgaatg gtataacaaa
    24001 gcaccaacag aacgttcatt aactatcatc cgtcctaccg ttatcttcgg tgaacgcaac
    24061 cgcggtaacg tctataactt gctgaaacag atcgctggcg gcaagtttat gatggtgggt
    24121 gcaggaacta actataagtc catggcttat gttggaaaca ttgttgagtt tatcaagtac
    24181 aaactgaaga atgttgccgc aggttatgag gtttataact acgttgataa gccagacctg
    24241 aacatgaacc agttggttgc tgaagttgaa caaagcctga acaaaaagat cccttctatg
    24301 cacttgcctt acccactagg aatgctgggt ggatattgtt ttgatatcct gagcaaaatt
    24361 acgggcaaaa aatacgctgt cagctcagtg cgcgtgaaaa aattctgcgc aacaacacag
    24421 tttgacgcaa cgaaagtgca ttcttcaggt tttgtggcac cgtatacgct gtcgcaaggt
    24481 ctggatcgaa ctctgcagta tgaattcgtt catgccaaaa aagacgacat aacgtttgtt
    24541 tctgagtaat gcagacgagt cagagaaaaa aggaccaggt tggactacga ttattcggaa
    24601 agaggtactg attaaattat cagtatgcgg aattccgagt aatccgagta acccagatgc
    24661 tgatggtgtg tagtgttacc ctatggttta agcaaagtta tgcggaacta agctttggta
    24721 tatagccata acgtttggct aacctatggc aataatttac gcactatcag tcacttagag
    24781 gtaggaaaga atgacgaatt taaaagcagt tataccggta gcaggtcttg ggatgcatat
    24841 gttgcctgcc actaaggcga ttcccaaaga gatgctaccg atcgtcgaca agccaatgat
    24901 tcagtacatt gttgacgaga ttgtggctgc agggatcaaa gaaatcctcc tggtaactca
    24961 tgcgtccaag aacgcggtcg aaaaccactt cgacacctct tatgaattag aatctcttct
    25021 tgaacaacgt gtgaagcgtc aactgttggc ggaagtacaa tctatctgtc cgccgggcgt
    25081 gactattatg aacgtgcgtc agggcgaacc tttaggttta ggccactcca ttttgtgtgc
    25141 gcgacctgcc attggtgaca acccatttgt cgtggtactg ccagacgtag tgatcgacga
    25201 cgccagcgcc gacccgctgc gctacaacct tgctgccatg attgcgcgct tcaatgaaac
    25261 gggccgtagc caggtgctgg caaaacgtat gccaggtgac ctctctgaat actccgttat
    25321 tcagaccaaa gagccgctgg atcgtgaagg taaaatcagc cgcattgttg aattcatcga
    25381 aaagccagat cagccgcaga cgctggactc agacatcatg gccgttggtc gctatgtgct
    25441 ttctgccgat atttggccgg aacttgaacg tactcagcct ggtgcatggg gacgtattca
    25501 gctgactgat gctattgccg aactggcgaa aaaacaatct gttgatgcaa tgctgatgac
    25561 aggtgacagc tacgactgcg gtaaaaaaat gggttatatg caggcgtttg tgaagtatgg
    25621 actacgcaac ctgaaagaag gggcgaagtt ccgtaaaggg attgagaagc tgttaagcga
    25681 ataatgaaaa tctgaccgga tgtaacggtt gataagaaaa ttataacggc ggtgaagatt
    25741 cgtggcgaaa gtaatttgtt gcgaatattc ctgccgttgt tttatataaa caatcagaat
    25801 aacaacgagt tagcaatagg attttagtca aagttttcca ggattttcct tgttcccaga
    25861 gcggattggt aagacaatta gcgtttgaat ttttcgagtt aagcgcgagt gggtaacgct
    25921 cgtcacatcg taggcatgca tgcagtgctc tggtagctgt aaagccaggg gcggtagcgt
    25981 gcattaatac ctctattaat caaactaaga gccgctaatt tcacagcatg ctctgaagta
    26041 atatggaata aaaaagtgaa gatacttgtt actggtggcg caggatttat tggttctgct
    26101 gtagttcgtc acattataaa taatacgcag gatagtgttg ttaatgtcga taaattaacg
    26161 tacgccggaa acctggaatc acttgctgat gtttctgatt ctgaacgcta tgtttttgaa
    26221 catgcggata tttgcgatgc tgctgcaatg gcgcggattt ttgctcagca tcagccggat
    26281 gcagtgatgc acctggctgc tgaaagccat gttgaccgtt caattacagg tcctgcggca
    26341 tttattgaaa ccaatattgt tggtacttat gtccttttgg aagccgctcg caattactgg
    26401 tctgctcttg atagcgacaa gaaaaatagc ttccattttc atcatatttc tactgacgaa
    26461 gtctatggcg atctgcctca tcctgacgaa gtaaataata aagaagaatt acccttattt
    26521 actgagacga cagcttacgc gccaagcagt ccttattccg catcaaaagc atccagcgat
    26581 catttagttc gcgcgtggaa acgtacctat ggtttaccga ccattgtgac taactgttcg
    26641 aataactatg gtccttatca ctttccggaa aaattgattc cactagtaat tcttaatgct
    26701 ctggaaggta aggcattacc tatttatggc aaaggggatc aaattcgcga ttggctgtat
    26761 gttgaagatc atgcgcgtgc gttatatacc gtcgtaaccg aaggtaaagc gggtgaaact
    26821 tataacattg gcggacacaa cgaaaataaa aacatcgatg ttgtgctgac tatttgtgat
    26881 ttgttggatg agattgtacc gaaagagcaa tcttatcgtg agcaaattac ttatgttgcc
    26941 gatcgtccgg gacacgatcg ccgttatgcg attgatgctg agaagattag ccgcgaattg
    27001 ggctggaaac cgcaggaaac gtttgagagc gggattcgga agacagtgga atggtacctg
    27061 tccaatacaa aatgggttga taatgtgaaa agtggtgcct atcaatcgtg gattgaacag
    27121 aactatgagg gccgcaagta atgaatatcc tccttttcgg caaaacaggg caggtaggtt
    27181 gggaactaca gcgttctctt gctcctctgg gtaacttgat tgctcttgat gttcactcca
    27241 ctgattattg tggcgatttc agcaacccag aaggtgtggc tgaaaccgtc aaaaaaattc
    27301 gcccagatgt tattgttaat gctgcggctc ataccgcagt agataaagct gagtcagaac
    27361 ccgaatttgc acaattactc aatgcgacca gcgttgaatc aattgcaaaa gcggctaatg
    27421 aagttggggc ctgggtaatt catttctcaa ctgactacgt attccctgga aatggcgaca
    27481 ctccatggct ggagactgat gcaaccgcac cactaaatgt ttacggtgaa accaagttag
    27541 ctggagaaaa agcattacaa gagcattgtg cgaagcacct tatttttcgt accagctggg
    27601 tatatgcagg taaaggaaac aattttgcca agacaatgtt acgtctggca aaagagcgcg
    27661 aagaattagc tgtcattaac gatcagtttg gtgcaccaac aggtgctgaa ctgctggctg
    27721 attgtacggc acatgcaatt cgtatggcac tgaataaacc agaagtcgca ggcttgtacc
    27781 atctggtagc cagtggtacc acaacctggc acgattatgc tgcgctggtt tttgaagagg
    27841 cacgaaaagc aggtattccc cttgcactca acaagctcaa ggcagtacca acaacagcct
    27901 atcctacacc agctcgtcgt ccacataact ctcgccttaa tacagaaaaa tttcagcaga
    27961 attttgcgct tgtcttgcct gactggcagg ttggcgtgaa acgaatgctc aacgaattat
    28021 ttacgactac agcaatttaa tagtttttgc atcttgttcg tgatggtgga gcaagatgaa
    28081 ttaaaaggaa tgatgaaatg aaaacgcgta aaggtattat tttagcgggt ggttctggta
    28141 ctcgtcttta tcctgtgact atggctgtca gtaaacagct attacctatt tatgataagc
    28201 cgatgatcta ttacccgctc tctacactga tgttggcggg tattcgcgat attctgatta
    28261 ttagtacgcc acaggatact cctcgttttc aacaactgct gggtgacggt agccagtggg
    28321 ggctaaatct tcagtacaaa gtgcaaccga ctccagatgg gcttgcgcag gcgtttatta
    28381 tcggtgaaga gtttatcggt ggtgatgatt gtgctctggt tctcggtgat aatatctttt
    28441 acggtcatga tctgccgaag ttaatggatg tcgctgttaa caaagaaagt ggtgcaacag
    28501 tatttgccta tcacgttaat gatcctgaac gctacggtgt cgttgagttt gataaaaacg
    28561 gtacggcaat aagcctggaa gaaaaaccgt tacaaccaaa aagtaattat gcggtaaccg
    28621 ggctttattt ctatgataac gacgttgtcg aaatggcgaa aaatcttaag ccttctgctc
    28681 gtggtgaact ggaaattacc gatattaacc gtatctatat ggaacagggg cgtttatctg
    28741 ttgccatgat gggccgtggt tatgcatggc tggacacggg ggcacatcaa agcctgattg
    28801 aggcaagcaa cttcattgca acaattgaag agcgtcaagg cttgaaagtt tcctgcccgg
    28861 aagaaattgc tttccggaaa gggtttattg atgctgagca ggtgaaagtg ttagccgaac
    28921 cgctgaagaa aaatgcttat ggtcagtatc ttctcaaaat gattaaaggt gattaataaa
    28981 atg
//
LOCUS       DAZGJT010000056        28421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZGJT010000056 DAZGJT010000000
VERSION     DAZGJT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.056"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..469
                     /locus_tag="ACHKFK_002708"
     CDS             <1..469
                     /locus_tag="ACHKFK_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053008.1"
                     /translation="MLFLAVLFTGFNFNAEAATVKQALSCNPEAWAEQPGACPSTYEL
                     YEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNINGTVWFQGDGCKANTC
                     GWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYLVKNYVAAVN"
     gene            485..895
                     /locus_tag="ACHKFK_002709"
     CDS             485..895
                     /locus_tag="ACHKFK_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053009.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            1116..1937
                     /locus_tag="ACHKFK_002710"
     CDS             1116..1937
                     /locus_tag="ACHKFK_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9053010.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     QLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            2019..2498
                     /locus_tag="ACHKFK_002711"
     CDS             2019..2498
                     /locus_tag="ACHKFK_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9053011.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPQWVALEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2514..2990
                     /gene="yeeS"
                     /locus_tag="ACHKFK_002712"
     CDS             2514..2990
                     /gene="yeeS"
                     /locus_tag="ACHKFK_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO9053012.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     KWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            3053..3274
                     /gene="yeeT"
                     /locus_tag="ACHKFK_002713"
     CDS             3053..3274
                     /gene="yeeT"
                     /locus_tag="ACHKFK_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9053013.1"
                     /translation="MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            3348..3716
                     /gene="cbeA"
                     /locus_tag="ACHKFK_002714"
     CDS             3348..3716
                     /gene="cbeA"
                     /locus_tag="ACHKFK_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO9053014.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            3805..4178
                     /gene="cbtA"
                     /locus_tag="ACHKFK_002715"
                     /pseudo
     CDS             3805..4178
                     /gene="cbtA"
                     /locus_tag="ACHKFK_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            4175..4369
                     /gene="yeeW"
                     /locus_tag="ACHKFK_002716"
     CDS             4175..4369
                     /gene="yeeW"
                     /locus_tag="ACHKFK_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9053015.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            4415..4495
                     /locus_tag="ACHKFK_002717"
                     /pseudo
     CDS             4415..4495
                     /locus_tag="ACHKFK_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4784..4930)
                     /locus_tag="ACHKFK_002718"
                     /pseudo
     CDS             complement(4784..4930)
                     /locus_tag="ACHKFK_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            4984..5166
                     /locus_tag="ACHKFK_002719"
     CDS             4984..5166
                     /locus_tag="ACHKFK_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO9053016.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(5267..5596)
                     /gene="tmaR"
                     /locus_tag="ACHKFK_002720"
     CDS             complement(5267..5596)
                     /gene="tmaR"
                     /locus_tag="ACHKFK_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO9053017.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(5768..6826)
                     /gene="yeeA"
                     /locus_tag="ACHKFK_002721"
     CDS             complement(5768..6826)
                     /gene="yeeA"
                     /locus_tag="ACHKFK_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO9053018.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(7025..7498)
                     /gene="sbmC"
                     /locus_tag="ACHKFK_002722"
     CDS             complement(7025..7498)
                     /gene="sbmC"
                     /locus_tag="ACHKFK_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO9053019.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(7617..8783)
                     /gene="dacD"
                     /locus_tag="ACHKFK_002723"
     CDS             complement(7617..8783)
                     /gene="dacD"
                     /locus_tag="ACHKFK_002723"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO9053020.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            8972..10399
                     /gene="sbcB"
                     /locus_tag="ACHKFK_002724"
     CDS             8972..10399
                     /gene="sbcB"
                     /locus_tag="ACHKFK_002724"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO9053021.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     RILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            complement(10442..10669)
                     /gene="tsuB"
                     /locus_tag="ACHKFK_002725"
     CDS             complement(10442..10669)
                     /gene="tsuB"
                     /locus_tag="ACHKFK_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO9053022.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(10683..11741)
                     /gene="tsuA"
                     /locus_tag="ACHKFK_002726"
     CDS             complement(10683..11741)
                     /gene="tsuA"
                     /locus_tag="ACHKFK_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO9053023.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVTVL
                     LLITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYARPQRKARLATAAAN"
     gene            complement(11920..13278)
                     /gene="plaP"
                     /locus_tag="ACHKFK_002727"
     CDS             complement(11920..13278)
                     /gene="plaP"
                     /locus_tag="ACHKFK_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO9053024.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(13268..13330)
                     /gene="yoeI"
                     /locus_tag="ACHKFK_002728"
     CDS             complement(13268..13330)
                     /gene="yoeI"
                     /locus_tag="ACHKFK_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO9053025.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(13545..14474)
                     /gene="yeeY"
                     /locus_tag="ACHKFK_002729"
     CDS             complement(13545..14474)
                     /gene="yeeY"
                     /locus_tag="ACHKFK_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9053026.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAMEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(14520..15344)
                     /gene="yeeZ"
                     /locus_tag="ACHKFK_002730"
     CDS             complement(14520..15344)
                     /gene="yeeZ"
                     /locus_tag="ACHKFK_002730"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9053027.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(15427..15681)
                     /gene="yoeB"
                     /locus_tag="ACHKFK_002731"
     CDS             complement(15427..15681)
                     /gene="yoeB"
                     /locus_tag="ACHKFK_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO9053028.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(15678..15929)
                     /gene="yefM"
                     /locus_tag="ACHKFK_002732"
     CDS             complement(15678..15929)
                     /gene="yefM"
                     /locus_tag="ACHKFK_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO9053029.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            16212..16262
                     /gene="hisL"
                     /locus_tag="ACHKFK_002733"
     CDS             16212..16262
                     /gene="hisL"
                     /locus_tag="ACHKFK_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO9053030.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    16238..16361
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            16408..17307
                     /gene="hisG"
                     /locus_tag="ACHKFK_002734"
     CDS             16408..17307
                     /gene="hisG"
                     /locus_tag="ACHKFK_002734"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO9053031.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLISMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            17313..18617
                     /gene="hisD"
                     /locus_tag="ACHKFK_002735"
     CDS             17313..18617
                     /gene="hisD"
                     /locus_tag="ACHKFK_002735"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO9053032.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASGRLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDTD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            18614..19684
                     /gene="hisC"
                     /locus_tag="ACHKFK_002736"
     CDS             18614..19684
                     /gene="hisC"
                     /locus_tag="ACHKFK_002736"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO9053033.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPIVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            19684..20751
                     /gene="hisB"
                     /locus_tag="ACHKFK_002737"
     CDS             19684..20751
                     /gene="hisB"
                     /locus_tag="ACHKFK_002737"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO9053034.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQVDFDGPHNLMMQIFTSQGVQFDEVLICPHLPTDECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            20751..21341
                     /gene="hisH"
                     /locus_tag="ACHKFK_002738"
     CDS             20751..21341
                     /gene="hisH"
                     /locus_tag="ACHKFK_002738"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO9053035.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESTGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            21341..22078
                     /gene="hisA"
                     /locus_tag="ACHKFK_002739"
     CDS             21341..22078
                     /gene="hisA"
                     /locus_tag="ACHKFK_002739"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO9053036.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            22060..22836
                     /gene="hisF"
                     /locus_tag="ACHKFK_002740"
     CDS             22060..22836
                     /gene="hisF"
                     /locus_tag="ACHKFK_002740"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO9053037.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            22830..23441
                     /gene="hisIE"
                     /locus_tag="ACHKFK_002741"
     CDS             22830..23441
                     /gene="hisIE"
                     /locus_tag="ACHKFK_002741"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO9053038.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(23538..24515)
                     /gene="wzzB"
                     /locus_tag="ACHKFK_002742"
     CDS             complement(23538..24515)
                     /gene="wzzB"
                     /locus_tag="ACHKFK_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO9053039.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDEKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSEALESMIKHEATRPLVFSSNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(24661..25827)
                     /gene="ugd"
                     /locus_tag="ACHKFK_002743"
     CDS             complement(24661..25827)
                     /gene="ugd"
                     /locus_tag="ACHKFK_002743"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO9053040.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVLK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELRDVADKVYTRDLFGSD"
     gene            complement(26072..27478)
                     /gene="gndA"
                     /locus_tag="ACHKFK_002744"
     CDS             complement(26072..27478)
                     /gene="gndA"
                     /locus_tag="ACHKFK_002744"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO9053041.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSVFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDKGEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(27597..>28421)
                     /gene="galE"
                     /locus_tag="ACHKFK_002745"
     CDS             complement(27597..>28421)
                     /gene="galE"
                     /locus_tag="ACHKFK_002745"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO9053042.1"
                     /translation="NLIFSNHDIHTVIHFAGSKSVGESISKPIHYYNNNVVASLVLIN
                     EMMKRGIYNLIFSSSATVYGNSNTMPVTENAPIGMTTNPYGTSKLMIEKILDDVTRAN
                     SSFRVTVLRYFNPVGAHFSGEIGEDPNGIPNNLMPYVCQVAIGKYKQVSVYGRDYPTK
                     DGTGVRDYIHVMDLAEGHVAALEHRNEGPNHKVYNLGTGKGYSVLDLLTTFERVTFRS
                     VPYVLSERRPGDIAECWSDPSKAYRELGWKAKRGLEEMVRDAWNWQQKNPNGYIKA"
BASE COUNT         7381 a         7287 c         6912 g         6841 t
ORIGIN      
        1 tatgttattt ctggcggttc tgttcacggg ctttaacttt aacgcagaag cggctaccgt
       61 aaaacaagct cttagttgta acccggaagc ctgggctgaa caacctggag cgtgtccatc
      121 aacgtacgag ttgtacgaag gtgacgctac ctacaaagcc gctatcgaca aagcattaaa
      181 accggtcgga ctgagcggca tgttcggtaa aggcggctat atggatggcc ctggtggagg
      241 tatcacgcca gtaaacatta acggtacagt ctggttccag ggcgacggtt gcaaagccaa
      301 tacctgcggc tgggacttta tcgtaacact ctataaccca aaaacccatg aagttgttgg
      361 ctaccgttac tttggtttag atgacccggc ctacctggtt tggttcggtg aaattggcgt
      421 gcatgaattc gcgtatctgg tgaaaaacta cgtagctgcg gttaactaat ttaaggagtc
      481 tatcatgaaa acttttatca aaactttact cgttgctgta actattctgt tctctgtctt
      541 cgctactgcg aaacaagtaa aactgccaaa taacatcaaa tacgttaata ctacagaagc
      601 gttttcctgt accgaaattg acggtatgaa ttgccagaca aagaatcagt tcaactataa
      661 agataacagc tatgttttcg tgcttgaacg tggtggtgcc tggtgctacg actacactgt
      721 ctcggtagtt aacctgaaaa ccgggaaagc acaaatgatt gaatacgggg acaatcaact
      781 gtgctcaggt agcaacaaac cgttcttcga aatcaaaaat ggcgtaccga cggtaggagt
      841 catcgacaca tccggaaaac ctgtcgttgt agcccaggac aaacttaaaa tctaaggagt
      901 aaaccatgcg tttctctgca cattattaat atcaaaggta aatacagccc cagccattgt
      961 taccggaaag ccgcttaatg cgtggctcgc cagtgaaagg cctctcagac aataaacctt
     1021 attcatttca cccgtcaggc cgcgtcttct ccgggagacg cggctttttt catttattgc
     1081 accagtaaat cttaaccacc gataaggagc aaagtatgcg attagcaagt cgttttggtt
     1141 atgctgcaaa ccagatacgc cgtgaccgtc cgctgacaca tgaagaactg atacgccatg
     1201 tacccagtat ttttggggaa gaccggcaca cctcccgcag tgaacggtat gcgtacattc
     1261 ccaccattac cgtcctggaa aatctgcagc gggaaggctt tcagccgttc ttcgcctgcc
     1321 agacccgtgt gcgcgacccg ggccgccggg gatacacaaa acacatgctg cgtctgcggc
     1381 gggccggaga gataaacgga gaacatgtcc ctgaaattat tcagctcaac tctcatgacg
     1441 gtacctccag ctaccagatg ctgccgggtt acttcaggtt cgtctgccag aacgggtgcg
     1501 tctgtggcca gtctctgggg gaagtgcgtg ttccacaccg gggaaatgta gtggagaaag
     1561 ttatcgaagg ggcttacgag gtggtgggcg tgtttgaccg gatagaggag aagcgtgatg
     1621 ccatgcagtc gctggtcctg ccgccaccgg cacgccaggc gctggcacag gcggcactga
     1681 cttaccgtta tggtgacgaa catcagcccg tcaccaccgc cgacattctg acgccgcgac
     1741 gccgggagga ttacggtaag gacctgtgga gcgcatatca gaccatccag gagaatatgc
     1801 tgaaaggcgg gatttccggt cgcagtgcaa aaggaaaacg tatccatacc cgggccattc
     1861 acagcattga taccgacatt aagctcaatc gcgcattgtg ggtgatggca gaaacgatgc
     1921 tggagagcct gcgctgatgc cgtttccctg agtgagggaa tcattcagct ttcgttcctg
     1981 aaaaacacac atttatttgt tatttaagga gttatctcat gaaaaccgtt tctcagaata
     2041 cccccacaat ttattcagct acaacaccag agaataatcc gcctcagttg gttgccagcc
     2101 tcgtccctga tgaacagcgc atcagcttct ggccgcagca ttttggcctc attccacagt
     2161 gggtggccct ggagccccgt gtcttcggct ggatggaccg tctgtgcgaa gactactgcg
     2221 gtggtatctg gaatctgtac accctgaaca acggcggggc atttatggca cccgaaccgg
     2281 atgacgatga tgacgaaaca tgggtactgt tcaatgccat gaacggtaac cgcgcagaaa
     2341 tgagcccgga agccgccggt attgccgcct gtctgatgac gtacagtcat catgcctgtc
     2401 gtacggagaa ttatgccatg acggtccatt attaccggtt gcgggattac gccctgcagc
     2461 atccggaatg cagcgccatt atgcgcatca tcgactgaat ggagagaagc acaatgcaac
     2521 aactttcctt tctgcccgga gagatgacgc ccggcgagcg cagcctcatt caacgggccc
     2581 tgaaaaccct ggaccgccat cttcatgaac ccggcgtggc cttcacctcc acccgtgcag
     2641 cacggaaatg gctgattctg aacatggcgg gactggagcg tgaagaattc cgggtgctgt
     2701 atctgaataa ccagaatcaa ctgattgccg gtgaaaccct ctttaccggc accatcaacc
     2761 gcacggaagt ccatccccgg gaagtgatta aacgcgccct gtaccacaat gccgctgccg
     2821 tggtgctggc acacaatcac ccctccggtg aagtcacacc cagtaaggca gaccgcctta
     2881 tcacggaacg tctggtacag gcactgggcc tggtggatat ccgggtgccg gaccatctga
     2941 tagtcggtgg caaccaggtt ttctcctttg ccgaacacgg tctgctttaa cccgtcacaa
     3001 ccacatcaca cctgttttca cttttatctt ctgtcttcag aggtatccca ttatgaaaat
     3061 tatcacccgt ggtgaagcca tgcgtattca ccaacaacat ccgacatccc gtctttttcc
     3121 gttctgtacc ggtaaatacc gctggcacgg cagtgctgaa gcgtataccg gtcgtgaagt
     3181 gcaggatatt cccggtgtgc tggccgtgtt tgctgaacgc cgtaaggaca gttttggtcc
     3241 gtatgtccgg ctgatgagcg tcaccctgaa ctgagtggga attctgatga gcagaattat
     3301 cgccactacc ggaccattct tagccgattt tctgtaagga ttttatcgtg tcagacacac
     3361 tccccgggac aacacttccc gacgacaatc acgaccgccc ctggtggggg ctgccctgca
     3421 ccgtgacgcc ctgtttcggg gcacgtctgg tgcaggaggg taaccggttg cattaccttg
     3481 ccgaccgcgc cggtatcaga ggcctgttca gcgatgcaga tgcgtaccac ctggaccagg
     3541 cctttccgct gctgatgaaa caactggaac tcatgctcac cagcggtgaa ctgaatcccc
     3601 gccatcagca taccgtcacg ctgtatgcaa aagggctgac ctgcaaagcc gataccctca
     3661 gcagttgtgg ttacgtttat ctggctgttt atccgacgcc cgaaatgaaa aattaactct
     3721 ccagaatagc cttctgctac ggcctggtgt tttcaccacg ccacttttcc atttttatat
     3781 ctgcatatca ggaaaatctt cagtatgaaa acattacctg tattacccgg gcaggcgacc
     3841 agttctcgcc cgtctcctgt tgaaatctgg cagatactgc tgtcccgact gctggaccag
     3901 cactatggcc tcacactgaa tgacacacct tttgccgatg aacgtgtgat tgagcagcat
     3961 attgaggcag gcatttcact gtgtgatgcg gtgaactttc tcgtggaaaa atacgcgctg
     4021 gtgctaccga ccagccggga ttcagcgact gtacccgctc tcagttaata aacagcatcg
     4081 atatcctccg ggctcgcagg gcgaccggcc tgatgacccg cgacaattac agaacggtaa
     4141 ataacattac cctgggtaag tatccggagg cgaaatgatg acactggaag ccgacagcgt
     4201 taacgtacag gcgctggata tggggcacat tgtcgttgac attgatggtg ttaatatcac
     4261 tgaactgatt aataaggccg ctgaaaaagg ttattcactc cgcgtggtgg atgaccgtga
     4321 ctctaccgaa acaccggcaa cttatgccag ccctcaccag ttgctgtaag acaatgcagt
     4381 gatgcagggt atagtgccat taaccacaga aacagcactg ttgtcctgta ttaagcaggc
     4441 aagagaagat gtccttaccc tgcgccatct gcagcttctg caccagaacc ggtgaatctc
     4501 actcaacagg cttcatttcc cctgacgcca gcctgaatac agctggcgtt ttcatttata
     4561 aacagaaagg aaaaccgata ataatggaat ggacgccccg accatgaagc aacggtagga
     4621 tattactgat aaaccttcca gtggaggtgc gttatggccg ttttaacgat tagcattgac
     4681 ctgacaaaaa atgaattcca aatccacggt ctgggtagga acagaaaaat ctaagctcag
     4741 aaaacggatt aaagtaagcg tcaacggagc accgtattga cgcttattta ttggtgagaa
     4801 ctacgttcca tggcaggagt tcgtcaacac ggttggaggg ccattccggc agtatgctca
     4861 gaatatggcg cagatacgct tccggatcga taccgttcag acggcaggtg cctgcttttc
     4921 cggcaacaag caggaatctc agcgctgaag caggaattag aggtaaaaac gccctaccgg
     4981 gccatgaatc acccggtcat tggagtagta accaaagcag atttagccag catggaacaa
     5041 atatctttgg tgaaaagttg gttacgggaa gctggagcgc acaacgtgtt agtaaccagt
     5101 gcagttaata acaatggagt tacagaactt tttgccttgc tgcatacaga agaaggctgt
     5161 tgttaatttc gattggttct cagatgagaa tgatgacggt gatacacaga taagaatcaa
     5221 tgagcaatga gggttgccgg gcaaccctca ttgaataaaa cgggaattac ttcgcttcgc
     5281 cgtttttcat ttcgcccata gctttcagct ttttggagat atcgcggcgt tctttggaga
     5341 gctcggcatt tttgatgatg taatcgtcaa cgcgatcttc atagtcacct ttcatgctgg
     5401 cgatgatgcc ctggattgct tcaacgctca tccctggctt gatgtaatcg ctcaggttgt
     5461 ccagcaacag gacgcgcttc tggttgtcac ggatcttttt ctcaacgtcc tgaatttcac
     5521 gttgcagttt gttcttacga cggaacagac gaacaaattc cagtacgtcc tggaatgaag
     5581 gcttggtagt ttccattttt atacccctga taatgtgaga gtcggattcg tttaagcaac
     5641 cgctattcgt tagggccaac attactcata gctgccgcag atgacaatgc ttttatccct
     5701 tttcactatc atacccttta tccttgctga atcgaagcag cagcaagatg attctgaagt
     5761 tcaggaatta tttgcgcaag gcccggcaaa tcagattcga tagcaactca agctgatgtg
     5821 ccgtctccat gttcagccac acataaccat agattggtgt ttccacaacc ttcaggtcat
     5881 ggtggttatt gagcaactga cgcagctctt ccacagcatc gttcaatttt tccgtattgg
     5941 caaaaaccgg ctgcggatta ccttcgtaca gcgcatgaac aaggctcagc agtatttgct
     6001 gcatcatatg ctgggtgtca cgaagttttt gcgcgttcaa taacacgaaa tggctggggc
     6061 gcgtggccca gtacgcattg atttgcaact ccagcataca aaccagattg cgattaatgg
     6121 tctggatacc ttcatatatc gattttggaa tacgggtttc tttgctggcg ggcgcaatca
     6181 gcccacgcat tttcacggca tcggtcagga gtttttgtag atggctttcc agacgtgggc
     6241 gttcgagtaa gttcggtgag aatgcagatt gataaacccg attatactcg gtcagacttt
     6301 tcgccagttg aatgcgccag tggatgaacg cccgttgtgg ccagataccg gtaaacaaca
     6361 ttgcgagtaa agagccgagg atcacatcgc cgcttcgcca taacgccgta tcaatttcac
     6421 ctgtcgggga accaacaaca attgccagcg tcaccccaat caataatcct tgatacggtt
     6481 tcttgcccag tgccagccaa ccgcaaagaa acatagccgc cgcgcaccag actaacatca
     6541 gcggtaacga aattaactcc agttgcagag cgataagacc taatatcgaa cccaacaccg
     6601 taccgccaat acgttcgaag gctcgaggga caacgttacc ccagaacgag atgggcccca
     6661 taatcaccac catggtgacc agcggccagg tactttccgg gatagtaaac aggcggataa
     6721 tgagaaaagt gagcaggaat gccagcgcga cccgagtacc atgcacaatg cggtaatggc
     6781 ggtatacccg gatttcaaac gggcttaatg acttatcggc acgcacgcag aaactccgta
     6841 atgaagatta aaagcgctca taactcatct gtcggccaga aggcgcgcct ctgaattatc
     6901 cgacggacaa accgggaaca gtgtaatttc ccggcaggag cgaaaaaatt gaaaggcgca
     6961 tcttactctt ttcgctttca aaaaaaagag tggtcatcgc gttaacacac cgccctgaga
     7021 tgaattagtg atgttttggc tgcaccgcaa catacatttc gatatcccag tacccatctt
     7081 ccgcgccatt gttcaaataa acctcgaagc agggctttgg taacatttca taagcactgt
     7141 cctgcaagag gctattaaag aactgatacc agggtttagc aaaatcatca ccgactacac
     7201 gagctaccgc caccgcatac tgaccacctg aaatttctgt cagaatgacg ccctcactgt
     7261 tttcggggag cgtaaagtaa cccggcaccg tcacgacggt gtcgcagcgt aatttttcgg
     7321 cgggtgtttc atctggattg tcgtaataga cagcaaccca ttccttcggc acaatatttt
     7381 tgctatctac ccacatcatc aactgctcaa aacctttctt taccgtctgt tcccacgggc
     7441 caacgagatg gaaacctgca acggtacgtt tatcttcctg cttaatctcg tagttcatga
     7501 cgcctccatt aatactgttt ttatatacag tatagttgca atattaaaac cacaaggaat
     7561 gagtgttgat tatgcgagca gactcgcact cctgccagtc tgctgcaaaa gaaaggtcag
     7621 gccttatggt ggaaataatc actaaggcga gaaaacatgc tgccttcccc gacagattcc
     7681 agggtaacca gcggccagtg cgccacctgt ttatcacggt cgtaaagttc aatttcccct
     7741 acccgctgat gggcgctaat tggcgcggtg agttctttac catcaagggt atatttggct
     7801 ttgatatgtg gaatttcggc tttcggtagt accatccaga actcttgttc tgttcccagg
     7861 gcgatatttt ctttatcgcc ataccatatg cgttccgtac cgaccttttt cccacggtgc
     7921 aaaatttgca ccgtagtaaa gttttgttgc ccccaacgca gtaattttct tgcctcttcc
     7981 tcacgacctt ttgcgctgtc agcacccatt accactgcaa tgagacgacg ctgcccatct
     8041 acagccgaag caatgagatt aaacccggca ccagaagtat gacccgtttt caggccgtca
     8101 acattcatgg ttttatccca caataacccg ttacggtttt gctgggtgat accgttccag
     8161 gtgagacttt tctcactgta catatgataa aactcgggct cgccgtggat gatagcacga
     8221 gaaagcacag ctaaatcata agccgagcta tgctggccag gtgcatccag accatgcact
     8281 gtttcaaaat gcgtatcctt gagatgcagc ttctcggcat agttgttcat catttcaaca
     8341 aactgccgtt gcccaccggc aatatagtca gccagagcaa cacaagcgtc atttccggaa
     8401 tccacaatta aaccacggct taaatcacgt accgatacgc gatcgccctc tttcaaaaac
     8461 atcagtgaag aaccgacaaa caccggatta tctttcgccc acgcatcgcg ccccacggtg
     8521 acaatatcgt ctggcgtaat gcgatgacta tcgatagcgc gatccacgac ataacccgtc
     8581 atcagctttg tcaggctggc gggattgcgc tgttgatgct cattacccgc ggtgaggatc
     8641 tgaccggtgg tgtaatccat caatacccag gacccggcat gaatctctgg aggctgaggt
     8701 gaaaaaggaa tgttttccgc cgcaaaacca gacgataagt taaaaacgaa caaagaagca
     8761 gcaataataa gacggcgttt caacagcaaa ccctcaggag tttcaaatag ctgttctttt
     8821 tacggaaata cttatgaact ggctggaata aagtgcaaga aaatgtgact accctctcat
     8881 ttttatctga catgatcttg tggcgctaaa gctgattagt acggtgatat ttgatactct
     8941 ggcagacagc agaaataacg gatttaacct aatgatgaat gacggtaagc aacaatctac
     9001 ctttttgttt cacgattacg aaacctttgg cacgcacccc gcgttagatc gccctgcaca
     9061 gttcgcagcc attcgcaccg atagcgaatt caatgtcatc ggcgaacccg aagtctttta
     9121 ctgcaagccc gcggatgact atttgcccca gcctggagca gtattaatta ccggtattac
     9181 cccgcaggaa gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc gtattcactc
     9241 gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt tcgacgacga
     9301 agtcacacgc aacgtttttt atcgtaattt ctacgatcct tacgcctgga gctggcagca
     9361 tgataactcg cgctgggatt tgctggatgt tatgcgtgcc tgttatgccc tgcgcccgga
     9421 aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg agcatttaac
     9481 caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg tgtacgccac
     9541 tattgcgatg gcaaagctgg taaaaacgcg tcagccacgc ctgtttgatt atctctttac
     9601 ccatcgcaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga aacccctggt
     9661 tcacgtttcc ggaatgtttg gggcatggcg cggcaatacc agctgggtgg caccgctggc
     9721 gtggcatcct gaaaatcgca atgccgtaat tatggtggat ttggcaggag acatttcgcc
     9781 attactggaa ctggatagcg acacattgcg cgagcgttta tataccgcaa aaaccgatct
     9841 tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc cggtgctggc
     9901 ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc gtcagcattg
     9961 cctcgataac ctgagaattc tgcgtgaaaa tccgcaagtg cgcgaaaaag tggtggcgat
    10021 attcgcggaa gccgaaccgt ttacgccttc agataacgtg gatgcacagc tttataacgg
    10081 ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg agccacgtaa
    10141 tttaccggca ctggatatca cttttgttga taaacgaatt gaaaaactgt tgttcaatta
    10201 tcgggcgcgc aacttcccgg ggacgctgga ttatgccgag cagcaacgct ggctggagca
    10261 ccgccgccag gtcttcacgc cagagttttt gcagggctat gctgatgaat tgcagatgct
    10321 ggcacaacaa tatgccgatg acaaagagaa agtggcgctg ttaaaagcgc tttggcagta
    10381 cgcggaagag attgtctaat ttgaagcctc cgccgctggt acggcggagg tcaatgttga
    10441 gtcaggcttt ttgaacggtg atgctccacg ctgcatcgcc aatctgctga taatcggtga
    10501 tggcatgccc ttcttctgcc gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa
    10561 actcaattac caactcgtcg ccgctggcca tttctgccag tgctgctttt gcttctatga
    10621 gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc tttacacctt
    10681 acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgagc atacacaagc
    10741 caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc ctgccaggtc
    10801 atcattgcag tcataactag tccattaccg atagagcaac cacctgcaat actggcaccg
    10861 aagcccatca gtacaccacc taacccgcta cgtaatgttg tttgtgcatc agctgcgcga
    10921 acgcggaact cacggctcgc tttggctgca ataaatgacc ccacgaagat ccctaacacc
    10981 agaaaaacgc cccagttaat gtatttcacg tcacccgcga ccagaaattg cagaatattg
    11041 gccgttgggg aagtgattcc taacccaaac atgcgtccgg ttgcttcact cagtggccag
    11101 gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa agggatgcca gcgtttttca
    11161 aacagaatat gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc
    11221 ttcttcaact ctttcatcac cacccagagc gttatcaaca gcaaaacggt aaccaacggc
    11281 cacacagaca aattaaaagt ctcagcaata gagttatgtt cagtactgta gtgctgcaag
    11341 gtttgattta aaccactggc atgtggagaa cgcatcactg cactcatcac catataagtg
    11401 aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt cccggtggca
    11461 catccacccg ccagaacaat tcccagcccg aagatatacc cacctataac agtaccaagc
    11521 cacgggaacg ccccggcttc gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc
    11581 acgctttgta ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt tttcgcgata
    11641 tacatatcgc gaaaaccacc tgtcaggcaa aaacgcccac gctgcatgac aaatcccagc
    11701 agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt aaccagatga
    11761 atgttaatga ggaaattatt catgactggt ggaatgcaga ccaataacca aattctctaa
    11821 attagaacaa atggttatta atgaggattt cgataaaaaa gaataaaaaa accggagcca
    11881 aatattcatt cgactccggt tgattattag aaaagatgat tatgctacgt cttcgtactg
    11941 cggaaccggg ttacggaagc tcttggtgac gcaagccagg taaatcagac cgatagcagc
    12001 ccagatcagc cccagaacca ttgagctttc ttccaggtta acccacagcg caccaacggt
    12061 cagcgcacca cacatcggca ggaacagata ctggaagtga tctttcagcg tcttattacg
    12121 cttctcacgg atccagaact gcgagatgac cgacaggtta acgaaggtga acgccaccaa
    12181 cgcaccaaag ttgatcagcg ccgtagccat caccaggtcg aagttgattg ccagcagggc
    12241 aatcgcgcca accaggatga tgttcatcgc cggagtacgc catttcgggt gtacataacc
    12301 gaagaagctt ttcgggaata cgccgtcacg acccattacg tacatcagac gcgctacgcc
    12361 tgcgtgcgcc gccataccgg acgccagtac ggtaatggtg gagaagatca gcgcgccaac
    12421 ctggaacgct ttacctgcaa cgtacagcat gatttcaggc tgtgacgcat ccggatcttt
    12481 aaagcgagag atatccggga agtacagctg caggaagtaa gttgcaaaga tgaagatcat
    12541 gccgccgatc agcgcggtca ggaaaatcgc acgcgggatc acgcgctctg catctttggt
    12601 ttcttccgac aggttgctga tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc
    12661 cccggtaatc atcgggataa catgcgcatc gccagaccag aatggacgag tgctcgccag
    12721 cgtaccagcg ccttcacctt caaatacgcc ataaacaacc atgcccagaa tcaccgcgat
    12781 cagcactacc tgcaacacga cgattacagt gttgaagttc gctacggatt tcagactacg
    12841 caggttaaag gcggtcataa aggccaccag cgccaccaca aacatccacg atgggatgga
    12901 aggcaccaga gcttcaaaat agattttcgc cagcagaatg ttgatcatcg gcgcgaacag
    12961 atagtcgagc agagaagacc aacccaccat aaagccgaca gtcgggctaa tggatttctg
    13021 ggcgtaagta tatgcagagc cagcagaagg atagcggcga accagcttcc cgtagctcag
    13081 agccgtaaac aggatcgcaa tcaatgcgaa cgcataggct gtcggcacat gaccatccgt
    13141 gaggcctgaa acgataccaa aagtatcaaa cagcgtcatc ggctgcatat aggcaagacc
    13201 catcattaca accggaacta acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg
    13261 agtaacgtta tgcgacatgg tcattctcct ttacggtgat aaccgtcgcg taagcaaaaa
    13321 attgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
    13381 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
    13441 gctgatgaaa cgtcggtttt tagtcatttt ttgacagcgg cgcattgtgc ctaaatgggg
    13501 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttaaat ggctgatttt
    13561 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatcccgc
    13621 caccagccgc tggcaagccc tgcggtctgt tcgttccagc caatccagac gggttcaaag
    13681 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgtgcc
    13741 agataacggg gtaaatagcc gcagccgagg ccgctaattt gcagctccag cttggtttta
    13801 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
    13861 gcctgcgcgg tatcccccac cacaatcgcc cgatatcgct taataatacg acgatttaac
    13921 ggctcttctt ccatggctaa cggatgatga ggcgcgaccg caaagacttg ttccagatcg
    13981 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacaata
    14041 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
    14101 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tcagtggcgc aagcagagaa
    14161 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
    14221 atcgcctgct tttccagttc ccgcacggta tgcagaactt cccgcccttt ctctaataac
    14281 atttttccgg tgcgggtgaa tttagcgcgg tgaccgctac gatcaagcaa ttgaatatta
    14341 aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg
    14401 gccgacgccg ccgcaaaact gccttctttt tctaaggcat cgaggatcat caacacatcc
    14461 agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt
    14521 tactccagcg gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa
    14581 atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga
    14641 ggttccagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct
    14701 ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg
    14761 ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg cgcggttttt
    14821 ccggcaaaga accgtccggg atgacgtccc ggtccgacca ggcccgcaag acgcagaata
    14881 tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca
    14941 ctgttggtca ccggattacg cggggtggtt tctttcaccg tgccttgcgc gtcgccatag
    15001 acagacgttg agctggtaaa aataatgcgg ggaatacgat gggccagcgc gctatccact
    15061 aactcttgta ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc
    15121 gtaatgacca gcgcatcggc atccatcagg gcatccagat cgtcagaatc gcaaactaac
    15181 tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca
    15241 tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc
    15301 ggcatgccta accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata
    15361 caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga
    15421 atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcgtcgg taaccgcgta
    15481 taccagacgg tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag
    15541 gggttctggc ttccccttac cttcaaatgg cgttctgcgg gtatctttga taagttcatt
    15601 gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc
    15661 ctcagaccag attagtttca ctcaataatg tccttttctg ttcctttgcc tgatttcagg
    15721 ctatcgattg agtccatcaa tctccgggcg ttagcggggg aacgcagtag ataagccgtc
    15781 tcttccagcg agttgtattc ttcgagtgac atcagaacac aagcctctcc attctgacga
    15841 gtaataagga tcggggcatg atcttcaacg gctttcatca ttgttgccga caaattctga
    15901 cgcgcttcgc tgtagctaat tgtacgcatg tcaatctcct cttttgtaca gttcattgta
    15961 caatgatggg cgttaattaa ctatttatta attagtttgt agatcaaggt attgtcagtg
    16021 agacgaaaat ccaggcttcg ctatttttgg tgccgtcagc ttagaggaca gtcctcttag
    16081 ccccctcctt tccccgctca ttcattaaac aaatccatcg ccataaaata tataaaaaag
    16141 cccttgcttt ctaacgtgaa agtggtttag gttaaaagac atcagttgaa taaacattca
    16201 cagagacttt tatgacacgc gttcaattta aacaccacca tcatcaccat catcctgact
    16261 agtctttcag gcgatgtgtg ctggaagaca ttcagatctt ccagtggtgc atgaacgcat
    16321 gagaaagccc ccggaagatc accttccggg ggctttttta ttgcgcggtt gataacggtt
    16381 cagacaggtt taaagaggaa taagaaaatg acagacaaca ctcgtttacg catagctatg
    16441 cagaaatccg gccgtttaag tgatgactca cgcgaattgc tggcgcgctg tggcattaaa
    16501 atcaatcttc acacccagcg cctgatctcg atggcagaaa acatgccgat tgatattctg
    16561 cgcgtgcgtg acgacgacat tcccggtctg gtaatggacg gcgtggttga cctcgggatt
    16621 atcggcgaaa acgtgctgga agaagagctg cttaaccgcc gcgcccaggg tgaagatcca
    16681 cgctacttta ccctgcgtcg tctggatttc ggcggctgcc gactttcact ggcaacgccg
    16741 gttgatgaag cctgggatgg cccgctctcc ttaaacggta aacgtatcgc cacctcttat
    16801 cctcacctgc tcaagcgtta tctcgaccag aaaggtatct cttttaaatc ctgcttactg
    16861 aacggttctg ttgaagtcgc cccgcgtgcc ggactggcgg atgcgatttg cgatctggtt
    16921 tccaccggtg ccacgctgga agctaacggc ctgcgcgaag tcgaagttat ctatcgctcg
    16981 aaagcctgcc tgatacaacg cgatggcgaa atggaagaat ccaaacagca actgatcgac
    17041 aaactgctga cccgtattca gggtgtgatc caggcgcgcg aatcaaaata catcatgatg
    17101 cacgcaccga ccgaacgtct ggatgaagtc atcgccctgc tgccaggtgc cgaacgccca
    17161 actattctgc cgctggcggg tgaccaacaa cgcgtagcga tgcacatggt cagcagcgaa
    17221 accctgttct gggaaaccat ggaaaaactg aaagcgctgg gtgccagttc aatcctggtc
    17281 ctgccgattg agaagatgat ggagtgatcg ccatgagctt taacacaatc attgactgga
    17341 atagctgtac tgcggagcaa caacgccagc tgttaatgcg cccggcgatt tccgcctctg
    17401 aaagcattac ccgcactgtt aacgatattc tcgataacgt gaaagcacgc ggcgatgagg
    17461 ccctgcggga atacagcgcg aagtttgata aaaccacggt taccgcactg aaggtgtctg
    17521 ctgaggaaat tgccgccgcc agcggacgcc tgagcgacga gctaaaacag gcgatggcgg
    17581 tggcagtaaa gaatattgaa accttccaca ctgcgcaaaa actgccgccg gtagatgttg
    17641 aaactcagcc aggcgtacgt tgccagcagg tcacgcgccc agtcgcttca gttgggttgt
    17701 atattcctgg cggctccgcc ccgctcttct caacggtatt aatgctggca actccggcgc
    17761 gtattgcagg ttgcaataaa gtggtgctgt gctcaccgcc gccgattgcc gatgagatcc
    17821 tttatgcggc gcagctgtgc ggtgtacagg acgtgtttaa cgtcggcggc gcacaggcca
    17881 ttgccgcact ggcgtttggt actgaatctg tgccgaaagt ggacaaaatc ttcgggccgg
    17941 gtaacgcctt tgtcaccgaa gcgaaacgtc aggtgagcca gcgtctggac ggtgcggcga
    18001 tcgatatgcc cgcaggcccg tcggaagtgc tggtgattgc tgacagcggc gctacgccag
    18061 atttcgtggc ttctgatttg ctttctcagg ctgaacacgg cccggactca caggtgattt
    18121 tactgacgcc cgatactgat atggcgcatc aagttgccga ggccgtcgaa cgccaactgg
    18181 cagaactgcc gcgtgccgaa accgcccgcc aggcactgag cgccagccgc ctgatcgtga
    18241 ctaaagattt agcgcagtgc gtagagattt ctaatcagta cggcccggag cacctgatca
    18301 ttcagacccg caacgcccgc gagctggtcg atggcatcac cagcgccggt tcggtatttc
    18361 ttggtgactg gtcaccggaa tcggcaggtg attacgcctc cggcaccaac cacgttctac
    18421 cgacttacgg ctacaccgcc acctgttcca gcctcggcct ggctgatttc cagaagcgta
    18481 tgaccgtgca ggagctgtcg aaagaaggtt tctccgcgct ggcttcgacc attgaaacac
    18541 tggccgccgc cgagcgcctg actgcccaca aaaatgccgt tactttgcgt gttaacgccc
    18601 ttaaggagca agcatgaaca ccgtgactat taccgattta gcgcgtgaaa acgtccgcaa
    18661 cctgacgccg tatcagtcgg cgcgtcgtct gggcggtaac ggcgacgtct ggctgaacgc
    18721 caacgaatac cccacagccg tggagtttca gctcactcag caaacgctca accgctaccc
    18781 ggaatgtcag ccgaaagcgg tgattgaaaa ttacgcgcaa tatgcaggcg taaaaccgga
    18841 acaggtgctg gtcagccgtg gcgcggacga aggtattgaa ctactgattc gcgctttttg
    18901 cgaaccgggt aaagacgcca tcctctactg cccgccaacg tacggcatgt acagcgtcag
    18961 cgctgaaacc attggcgtcg agtgccgcac tgtgccgacg ctggaaaact ggcaactgga
    19021 cttacagggc atttccgaca agctggacgg cgtaaaagtg gtctatgttt gcagccccaa
    19081 caacccgacc gggcaactga tcaacccgca ggattttcgc actctgctgg agttaacgcg
    19141 cggtaaggcg attgtggttg ccgatgaagc ctatatcgag ttttgcccac aggcatcgct
    19201 ggctggctgg ctggcggaat atccgcacct ggctattttg cgcacactgt cgaaagcttt
    19261 tgctctggcg gggcttcgtt gcggatttac gctggcaaac gaagaagtca tcaacctgct
    19321 gatgaaagtg atcgccccct acccgctctc gacgccggtt gccgacattg cggcccaggc
    19381 gttaagcccg caggggatcg tcgccatgcg cgaacgggta gcgcaaatta ttgcagaacg
    19441 cgaatacctg attgccgcac tgaaagagat ccccatcgtg gagcaggttt tcgactccga
    19501 aaccaactac attctggcac gctttaaagc ctccagcgca gtgtttaaat ctttgtggga
    19561 tcagggcatt atcttacgtg atcagaataa acaaccctct ttaagcggct gcctgcgaat
    19621 taccgtcgga acccgtgaag aaagccagcg cgtcattgac gccttacgtg cggagcaagt
    19681 ttgatgagtc agaagtatct ttttatcgat cgcgatggaa ccctgattag cgaaccgccg
    19741 agtgattttc aggtggaccg ttttgacaaa ctcgcctttg aaccgggcgt gatcccgcag
    19801 ctgctgaagc tgcaaaaagc gggctacaag ctggtgatga tcaccaatca ggatggtctg
    19861 ggaacacaaa gtttcccgca ggtggatttc gatggcccgc acaacctgat gatgcagatc
    19921 ttcacctcgc aaggcgtgca gtttgatgaa gtgctgattt gtccgcacct gcccaccgat
    19981 gagtgcgact gccgtaagcc gaaagtaaaa ctggtggagc gttatctggc tgagcaagcg
    20041 atggatcgtg ccaacagtta tgtgattggc gatcgcgcga ccgatattca actggcggaa
    20101 aacatgggta ttaatggttt acgctacgac cgcgaaaccc tgaactggcc gatgattggc
    20161 gagcaactca ctaaacgaga ccgttacgcc catgtagtgc gcaacaccaa agagacgcaa
    20221 attgacgtcc aggtgtggct ggatcgcgaa ggtggcagca agattaatac cggcgttggc
    20281 ttctttgatc acatgctgga tcagatcgcc acccacggcg gtttccgtat ggaaatcaac
    20341 gtcaaaggcg acctctatat cgacgatcac cacaccgtcg aagataccgg cctggcgctg
    20401 ggtgaagcgt taaaaattgc ccttggcgac aaacgcggta tttgccgctt tggttttgta
    20461 ctgccgatgg acgaatgcct tgcccgctgc gcgctggata tctctggtcg cccgcacctg
    20521 gaatataaag ctgaatttac ctaccagcgt gtgggcgatc tcagcaccga gatgatcgag
    20581 cacttcttcc gttcactttc atacaccatg ggcgtgacgc tacacctgaa aaccaaaggt
    20641 aaaaacgatc accaccgtgt agagagcctg ttcaaagcct ttggtcgcac cctgcgccag
    20701 gccatccgcg tggaaggcga caccctgccc tcgtcgaaag gagtgctgta atgaacgtgg
    20761 tgatccttga taccggctgc gccaatttga actcggtgaa gtctgccatt gcgcgtcacg
    20821 gttatgaacc caaagtcagc cgtgacccgg acgtagtttt gctggccgat aaactatttt
    20881 tacccggcgt aggcaccgca caagcagcga tggatcaggt gcgtgagcga gaactgtttg
    20941 atctcatcaa agcctgtacc caaccggtgc tgggcatctg cttagggatg caactgctgg
    21001 ggcggcgcag cgaagagagc accggcgtcg acttgctggg catcatcgac gaagacgtgc
    21061 cgaaaatgac cgactttggt ctgccactgc cacatatggg ctggaaccgc gtttacccgc
    21121 aggcaggcaa ccgcctgttt caggggattg aagacggcgc gtacttttac tttgttcaca
    21181 gttacgcaat gccggtcaat ccgtggacca tcgcccagtg taattacggc gaaccgttca
    21241 ccgcggcggt acaaaaagat aacttctacg gcgtgcagtt tcacccggag cgttctggtg
    21301 ccgctggcgc taagttgctg aaaaacttcc tggagatgta atgattattc cggcattaga
    21361 tttaatcgac ggcactgtgg tgcgtctcca tcagggcgat tacggcaaac agcgcgatta
    21421 cggtaacgac ccgctgccgc gcttacagga ttacgccgca cagggtgccg aagtgttgca
    21481 cctggtggat ctgaccgggg caaaagatcc ggcaaaacgt caaatcccgc tgattaaaac
    21541 cctggtggcg ggcgttaacg tccctgtaca ggttggcggc ggcgtgcgta gcgaagaaga
    21601 cgtggcggcg ttactggaag cgggcgttgc gcgcgtggtg gtcggctcca ccgcggtgaa
    21661 atcaccagaa atggtgaaag gctggtttga acgtttcggt gccgatgcct tagtgctggc
    21721 gctggatgtc cgtattgacg agcaaggcaa caagcaggtg gcagtcagcg gctggcaaga
    21781 gaactcgggc gtttcactgg aacaactggt ggaaacctat ttgcccgtcg gcctgaaaca
    21841 tgtgctgtgt accgatatct cgcgcgacgg cacgctggca ggctctaacg tctctttata
    21901 tgaagaagtg tgcgccagat acccgcaggt ggcatttcag tcatccggcg gcattggcga
    21961 cattaacgat gttgctgccc tgcgtggtac tggcgttcgc ggcgtaatag ttggtcgtgc
    22021 attactggaa ggtaaattca ccgtgaagga ggccatcgca tgctggcaaa acgcataatc
    22081 ccatgtctcg acgttcgtga tggtcaggtg gtgaaaggcg tacagtttcg caaccatgaa
    22141 atcattggcg atattgtacc gctggcaaaa cgctacgctg aagaaggtgc agacgaactg
    22201 gtgttctacg atatcacagc ttccagcgat ggccgtgtgg tagataaaag ctgggtatct
    22261 cgcgtggcgg aagtgatcga cattccgttc tgcgtggcgg gtgggattaa gtctctggaa
    22321 gatgccgcga aaattctttc ctttggcgcg gataaaattt ccataaactc gcctgcgctg
    22381 gcggatccga cgttaattac tcgcctggcg gatcgttttg gcgtgcagtg tattgtggtg
    22441 ggtattgata cctggtacga cgctgaaacc ggtaaatatc atgtgaatca atataccggc
    22501 gatgaaagcc gtacccgcgt cactcagtgg gaaacgctcg actgggtaca ggaagtgcaa
    22561 aaacgcggtg ccggagaaat cgtcctcaac atgatgaatc aggacggcgt gcgtaacggt
    22621 tacgacctcg aacagctgaa aaaagtgcgt gaagtttgcc acgtcccgct gattgcctcc
    22681 ggtggcgcgg gcaccatgga acacttcctc gaagccttcc gcgatgccga cgttgacggc
    22741 gcgctggcag cttccgtatt ccacaaacaa ataatcaata ttggtgaatt aaaagcgtac
    22801 ctggcaacac agggcgtgga gatcaggata tgttaacaga acaacaacgc cgcgaactgg
    22861 actgggaaaa aaccgacgga ctgatgccgg tgattgtgca acatgcggta tctggcgaag
    22921 tgctaatgct gggctatatg aacccggaag ccttagacaa aaccatcgaa agcggcaaag
    22981 tcaccttctt ctcgcgcact aaacagcgac tgtggaccaa aggcgaaaca tcgggcaatt
    23041 ttctcaatgt ggtgagtatt gccccggact gcgacaacga cacgttactg gtgctggcga
    23101 atcccatcgg gccaacctgc cacaaaggca ccagcagctg cttcggcgac accgctcacc
    23161 agtggctgtt cctgtatcaa ctggaacaac tgctcgccga acgcaaatct gccgacccgg
    23221 aaacctccta caccgccaaa ctgtatgcca gcggcaccaa acgcattgcg cagaaagtgg
    23281 gcgaagaagg cgtggaaacc gcactggccg cgactgtaca tgaccgcttt gagctgacca
    23341 acgaggcgtc tgatttgatg tatcacctgc tggtgctgtt gcaagatcag gatctggatt
    23401 taactacggt aattgagaac ctgcgtaaac ggcatcagtg agttgcgggg taagcggatg
    23461 cgatattgtt gtcgcatccg gcaaaaaaaa cgggcaaggt gtcaccaccc tgcccttttt
    23521 ctttaaaacc gaaaagatta cttcgcgttg taattacgca gagcgttacg ccccagcaca
    23581 atccccgcgc caaccatgcc gcccagcagc accgccagaa tcaaggtaat tgcctttttc
    23641 gggctatcgc gacgaatagg taacgtcggt ttcattacat agcggtaagc atgaatatca
    23701 agtttatcaa cgtcaagatt gtcgatatcc agcaggtttt gacgagtctg atagtagttt
    23761 gatgagaaca ccaacggacg agtcgcctcg tgcttaatca tcgactccag cgcttcgctc
    23821 cccaaaagga acatagtatc ttgggttacg tcctgtgtct gctgaatctg cggctttgtc
    23881 acctgcgcct gattcgcata ctgcaacgct tcctgaatct gacggatacg cagatctttc
    23941 tgctcctgcg cgactacttc ctgagttctt aaagagtcct gcaagttttt ccgtcccaga
    24001 gcaatgttgt ctttgagatc cttttctagc tcttgattca ctttttcatc aacttgctga
    24061 atgtattggg ccaacttcat ttgtgcgccc tctgctgttt gcccaacata agaaacagtc
    24121 aatggtaatt gctggttctt aacagaaggt tcgatggtaa gtttctctgg ctcttcctga
    24181 ttatccagcg tttctgctaa tgcagagaag gcagaactga agcgaccaat taacgtctcc
    24241 tgcaaatccg atactttcgg tgcagcctga ccatatataa cattcatggc attgttatag
    24301 ccagcaattt gccccacgtc gggctgagtg ataattgctg ttgacgtcca tttctccttc
    24361 gctactgcca aataaccaat agccagggca atagctacaa tgacggaaat gatgattgtc
    24421 atcttgccac gccacaactg cactagtaaa tcaatcaaat caatctgttc cgggtcatgg
    24481 ttttgcccag aaacattatt attttctact ctcatcatta ccctaactga cggaaatttc
    24541 caagtccgca tagtttagcg ataatccccg tgaatgctat aggataagtg ataaaaataa
    24601 tgaattgttg cgtaagtaac aaaagacaat cagggcgtaa atagccctga taacaagatg
    24661 ttagtcgctg ccaaagagat cgcgggtgta gaccttatct gccacatccc taagctcttc
    24721 tgccatacgg ttggaaataa tgacgtcggc ttgttgtttg aaggtggcga gatcacgttc
    24781 caagcgagag ttgaagaatg agtcttcttt catcactggc tcgtagatga tcacttcaac
    24841 gcctttcgcc ttgatacgct tcataatccc ctgaatagaa gacgcacgga agttatctga
    24901 accgctcttc ataatcagac gataaatacc caccacttgc ggtttgcgtg acaaaatggc
    24961 atcggcaata aaatccttac gcgtgcggtt tgcatcgaca attgccgaga tcaggttatt
    25021 cggcacagac tgatagttcg ccagtaactg cttggtatct ttcggcaagc aataaccacc
    25081 ataaccaaac gacgggttgt tgtaatggtt gccaatacgc ggatcgagac aaacgccttc
    25141 gataatctgg cgagtattca gacctaaact ttctgcgtag ctatccagtt cgttaaagta
    25201 cgccacgcgc atcgccaggt aggtgtttgc aaaaagttta atcgcttctg cttcagtgga
    25261 gtcggtaaac agggtcggga tattttgctt aatcgcgcct tcctgtaaca acgcagcaaa
    25321 acgttctgcg cgttctgaac gctcacctat gacaatacgt gaaggatgga gattatcgta
    25381 aagggcttta ccttcacgga gaaattccgg agagaaaata atattttcag tacgatattt
    25441 cttatgcatc gcttcggtaa aaccaacggg aaccgttgat ttgatgacca taaccgcata
    25501 aggatttatc tcaactacgt ctttaagtac tgattctaca ctggatgtat tgaaataatt
    25561 agttttagga tcatagtcgg ttggagtggc gatgatgaca taatcagcat cccggtaggc
    25621 ttcattttta tctaatgtgg cattaaagtg tattttatct gattgcaaaa actgctgaat
    25681 ttccttatca acaataggag atatccgatc attcagcata gcaacgcgtg acggtaaaat
    25741 atctaatgcc acaacctcat gattttgtgc gattagaagc ccgtttgaca agcctacata
    25801 gccagtaccg gaaatggtga ttttcatttc atgctctcag aattaactta actgtgaatc
    25861 atgatgtttt tagcatcctg ataagagcta aaagttttaa cgctacgttg tcagatggtc
    25921 aacgcaacga aaaacaattc agattttctt tataagaata gctgatattt attaagttaa
    25981 tattaagcag tgaaatttag tctataagat tattggcaaa aaaagcccag caatatatgc
    26041 cgggcctcaa tttattgttg tttaagtcag attaatccag ccattcagta tggaacacac
    26101 cttctttatc aatgcgctta taagtatgtg caccgaaata gtcacgctgt gcctggatca
    26161 ggttcgcagg cagaacagcg gcacggtagc tgtcataata ggcaaccgca gcggcgaagg
    26221 tgggaaccgg gataccgttc tgtactgcat aagcaacaac atcacgcagc gcctgctgat
    26281 agtcatcggc aatttgttta aagtacggag ccagcagcag gttagcgatc tgcggatttt
    26341 cggcatatgc atcagtgatt ttctgcagga actgcgcacg gatgatgcag ccagcacgga
    26401 aaattttcgc gatttcgccg tagttcagat cccagttgta ctcttcagac gccgcacgca
    26461 gctgagagaa gccctgagcg taagaaacga ttttgcccag atacagagca cgacgaactt
    26521 tttcgatgaa ctcacctttg tcgccagctg gctgcgcttg cgggccagag agaactttag
    26581 atgcggcaac acgctgctct ttcagagaag agatataacg tgcaaacaca gactcggtaa
    26641 tcagcgacag cggttcgccg agatccagcg cgctctggct ggtccattta ccggtacctt
    26701 tgttagccgc ttcatccagg atcacatcaa ccaggtagtt accgtcttca tcttttttag
    26761 tgaagatatc tttggtgatg tcgatcaggt agctgctcag ttcaccgtta ttccactcgg
    26821 taaaggtctg cgccagttct tcgttagaga gattcaggcc gcctttcagg agagaatagg
    26881 cttcagcaat cagctgcata tcaccgtatt caataccgtt gtgaaccatt tttacatagt
    26941 gacccgcgcc atcggcacca atataggtaa cgcacggttc gccatcttca gcaacggcgg
    27001 cgattttggt caggatcgga gcaaccagtt cataggcttc tttctggcca ccaggcataa
    27061 tggaaggacc tttcagcgcg ccctcttcac cgccggaaac accggtaccg atgaagttaa
    27121 agccttcggc agaaagctca cggttacggc gaatggtgtc ctggaagaag gtattaccac
    27181 catcaatgat gatgtcacct ttatcgaggt atggcttgag ggaatcaata gcagcatccg
    27241 tgccagcacc tgctttcacc attaacagga tgcgacgagg cgtttccaga gattcaacaa
    27301 actctttcac cgtatagtaa ggaaccagtt tcttgcctgg attttcggca atcacttctt
    27361 ccgtcttctc acgggagcgg ttgaaaacag agacggtata accacggctt tcgatgttga
    27421 gcgctaggtt gcgccccatc actgccatac cgacgacgcc gatctgttgc tttgacattg
    27481 tttactcctg tcagggtgat actgcgaaac ataactcagg tcaatgtaga tgctatatta
    27541 actcaggata cataaaatcg ataattttta ccgttgacaa aaataccata gagcattcat
    27601 gcttttatat aaccgttcgg attcttttgt tgccaattcc aggcatctcg aaccatttct
    27661 tccaacccac gcttcgcttt ccatccaagt tccctataag ccttagaagg atcagaccaa
    27721 cattcggcga tatctccagg gcgtctttca ctcaaaacgt aaggtacgga tcgaaaggtt
    27781 actcgttcaa aagtggtcaa aagatccaaa acagaatacc cctttcctgt tccgaggttg
    27841 taaactttat gattgggacc ttcgtttcta tgttccaacg cagcaacatg accttcagcg
    27901 agatccatta catggatata atcacggaca ccagtgccat cctttgttgg gtaatctctt
    27961 ccatatactg aaacttgttt atatttacca attgccacct gacaaacata tggcataaga
    28021 ttatttggaa taccatttgg atcctcacca atctctccag agaaatgagc acccacaggg
    28081 ttaaaatacc tcaaaaccgt cactctaaag ctcgaatttg cccttgtaac atcatctaat
    28141 attttttcaa tcataagttt agaagtgcca taaggatttg tagtcatccc aataggtgca
    28201 ttttcagtaa ctggcatggt attcgagtta ccgtatactg ttgcagatga gctaaaaatt
    28261 aaattataaa tacctctttt catcatttca ttaataagaa caaggctagc tacaacattg
    28321 ttattatagt aatgaattgg ttttgatata gactccccaa cagatttaga accagcaaaa
    28381 tgaataaccg tatgaatatc atgattagaa aaaataagat t
//
LOCUS       DAZGJT010000057        28287 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZGJT010000057 DAZGJT010000000
VERSION     DAZGJT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.057"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(133..1038)
                     /locus_tag="ACHKFK_002746"
     CDS             complement(133..1038)
                     /locus_tag="ACHKFK_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9053043.1"
                     /translation="MFISDETLRVIHLVARHQSITTAAEQLNKVPSAISYTIKKAEES
                     LGVELFIRKGRYIELSPAGSYFIEHSKTILGDLEALKRNTVLIHDGIERELTIAVNNI
                     IPGDLLVAFIRDFERQFTSTTLTVDLEVYNGCWDALYSKRADLVYGAPHAVPSSEGII
                     SEPVGQMEWDFVVSPLHPLAAKRHPLENSELRHYPAVCIRDTSVNFPPMQAWLLEGQK
                     PIFVPDFATAIALIEQNVGIGYIPHHLALPLLNSGKLLKKPMREHKHATKLFLAARSD
                     GMGKACQWCIEYLRNPQLMTRFVFN"
     gene            complement(1116..2339)
                     /locus_tag="ACHKFK_002747"
     CDS             complement(1116..2339)
                     /locus_tag="ACHKFK_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO9053044.1"
                     /translation="MRKKEENLNTASGLRIAMILLGIAVTPVLLSSSSLGNQLSSSSL
                     ISVVLLGGVILTLLSAITISVGEKARLPTYGIVKYSFGEKGAIAINILMAISLFGWIA
                     VTANMFGHSVHDLLAQHGLEVPLALLVAAGCVIFVASTAFGFAVLGKIAQVAVPVIAL
                     VLCYILYVATHTEVAVPAAIVEMNTGVAVSTVVGTIIVLVATLPDFGSFVHNRKHALI
                     AAGVTFLVAYPLLYWAGATPSAISGQGSLLGAMAVFGAVLPAALLLIFACVTGNAGNM
                     FQGTLVVSTLLTRFPKWQITVALGILSAIVGSMDIMAWFIPFLLFLGIATPPVAGIYI
                     ADFFLYRRNGYQESVLAQESQIKVLTFAAWIIGAAVGFMTVKGLFTLTTIPSVDSILV
                     ACIAYAILSRASQHR"
     gene            complement(2365..2754)
                     /locus_tag="ACHKFK_002748"
     CDS             complement(2365..2754)
                     /locus_tag="ACHKFK_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA amidotransferase"
                     /protein_id="HFO9053045.1"
                     /translation="MTTTLLAVNGTLMRGLELNPNMQKAGGIFVREDRTDAHYRLWSI
                     NDRHPGMIRVNEGGTHVDVEIWQLPLASFAALLMSEPAGLAIGKIKLADGSEVLGVLA
                     ENWLTEGQREITELGSWRKYTGHFHTV"
     gene            complement(2771..3727)
                     /gene="arcC"
                     /locus_tag="ACHKFK_002749"
                     /pseudo
     CDS             complement(2771..3727)
                     /gene="arcC"
                     /locus_tag="ACHKFK_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606533.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(3720..5144)
                     /locus_tag="ACHKFK_002750"
     CDS             complement(3720..5144)
                     /locus_tag="ACHKFK_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9053046.1"
                     /translation="MNTLFNQPLKVVNAGLHSFADNIQHAGGSAIALNWQPPAQGDID
                     AGLDLASLLRHPLVENANQIAMTRYLEAQPVLVDVMLAKEAIPAMAEQKRILHSGPPI
                     AWEEMCGPVKGAIIGAMLYEGWATSQQDAENQINAGEIDLAPCHHYHAVGPMAGIISP
                     SMPLWVVENKTNGHRTFSNFNEGLGKVLRFGANNDEVLNRLAWMRDELAPAMKAAIAQ
                     HGELELKPLMAQALHMGDEVHNRNAAATGLLIKRLLPALLACSLPQEHIQRVVAFITG
                     NDHFFLNLSMAACKAMMDAAANVPFSSMVTVMARNGVNFGIRLSSTGDRWFQAPANAV
                     EGLFFPGFGVDDAAADLGDSAITETAGVGGFAMASSPAIVKFVGGTPADATNNSRRMQ
                     AITLGGNPAFTLPALNFAPTAAGIDARKVTDRGILPVINTGIAHKQAGVGQIGAGITT
                     APMACFVAAVRALAEIVVKENHHG"
     gene            complement(5141..6700)
                     /gene="fdrA"
                     /locus_tag="ACHKFK_002751"
     CDS             complement(5141..6700)
                     /gene="fdrA"
                     /locus_tag="ACHKFK_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9053047.1"
                     /translation="MKVKYKVFSNLYQDSVSLMQISAQISKLPGIQQASVVMGTPNNL
                     EQLRDAGLGNEINASPNDLVIAVMGEEDICNEALALAQQRLTSKPDDETDSGIKTPEK
                     VSLEMALEAEPEANLALISVPGDYAAAEAIKALNLGMNVMMFSDNVSIGQEKSIKTLA
                     RERQRIVMGPDCGTAIVNGIPLGFANVVKRGAIGVIGASGTGLQEVTCRIDQLGAGIS
                     QALGTGGHDLSEEIGGISMLFALDALAQDDETRVIVLISKPPSPIVAQTILERAEACG
                     KPVVVNFLGANPHDLARPNITAATTLASAADIAVALLNVQPLPAVETDVPCDDLTMLQ
                     NACQRLPAHRQAIRGVFAGGTFCYEAQLICQQKGFSAASNTPVAGNRALANIWQSEDH
                     TLIDMGDDDFTRGKPHPMIDPTLRNQRLLNELNDSHTAVVLFDLVLGYGASTTPASEL
                     LDQLSHIDMNNVPLLIAHVCGTEADPQIRSQQISALQNAGVIIASSNAQAALWASTVA
                     QTQLQKKGLNA"
     gene            complement(6795..7655)
                     /locus_tag="ACHKFK_002752"
     CDS             complement(6795..7655)
                     /locus_tag="ACHKFK_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9053048.1"
                     /translation="MVQVTLCASSLGYLFPQNETQDLRLHSAFKHAVNLYSDAGTFIT
                     LLCAQTYLNLPDAARVWLPECWDWRREIAHSDPIQLTPGLLRTPRFCVALDNATLWQS
                     PFVGRMLTFEAFPLVFQHYPTMASQRLLFCLEHNVQSTLHLPDSLTHQGLAIMEHPDA
                     LERQVPQLIGFGKGLTPDGDDYLLGYLAALWLWQLPAPLADHQYRLQQAIDQHAHNTT
                     DISRHYLERALQGYFSEPICQLLAQLVGSASAMTIASCAEQVMQFGATSGVDCLAGML
                     HGFRTLNTMN"
     gene            complement(7661..8329)
                     /locus_tag="ACHKFK_002753"
     CDS             complement(7661..8329)
                     /locus_tag="ACHKFK_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HFO9053049.1"
                     /translation="MTQSIFQAQPFELPFDPRTTALVMIDMQRDFVEAGGFGEALGND
                     VSLVRTAIAPCTEVLAAARQKGIMVIHTREGHRADLSDCPPAKLTRGGKTFIGEPGPM
                     GRILVRGEAGHDIIPELYPVAGEPVIDKPGKGAFYQTDLHLILQNHGIKTLIVCGVTT
                     EVCVTTTVREANDRGYECIIPEDCVGSYFPEFQKYALEMIKAQGAIFGWVTDSKAIIA
                     GLEG"
     gene            complement(8586..9395)
                     /locus_tag="ACHKFK_002754"
     CDS             complement(8586..9395)
                     /locus_tag="ACHKFK_002754"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO9053050.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGLLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRRA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFTQYGVKK"
     gene            9657..10418
                     /gene="udp"
                     /locus_tag="ACHKFK_002755"
     CDS             9657..10418
                     /gene="udp"
                     /locus_tag="ACHKFK_002755"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO9053051.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            10559..11986
                     /gene="rmuC"
                     /locus_tag="ACHKFK_002756"
     CDS             10559..11986
                     /gene="rmuC"
                     /locus_tag="ACHKFK_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO9053052.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFVDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            12081..12836
                     /gene="ubiE"
                     /locus_tag="ACHKFK_002757"
     CDS             12081..12836
                     /gene="ubiE"
                     /locus_tag="ACHKFK_002757"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO9053053.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKTMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            12850..13455
                     /gene="ubiJ"
                     /locus_tag="ACHKFK_002758"
     CDS             12850..13455
                     /gene="ubiJ"
                     /locus_tag="ACHKFK_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO9053054.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLAALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKALRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            13452..15092
                     /gene="ubiB"
                     /locus_tag="ACHKFK_002759"
     CDS             13452..15092
                     /gene="ubiB"
                     /locus_tag="ACHKFK_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO9053055.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKPLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            15171..15440
                     /gene="tatA"
                     /locus_tag="ACHKFK_002760"
     CDS             15171..15440
                     /gene="tatA"
                     /locus_tag="ACHKFK_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO9053056.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            15444..15959
                     /gene="tatB"
                     /locus_tag="ACHKFK_002761"
     CDS             15444..15959
                     /gene="tatB"
                     /locus_tag="ACHKFK_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO9053057.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNETAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            15962..16738
                     /gene="tatC"
                     /locus_tag="ACHKFK_002762"
     CDS             15962..16738
                     /gene="tatC"
                     /locus_tag="ACHKFK_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO9053058.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIISVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            16780..17562
                     /gene="tatD"
                     /locus_tag="ACHKFK_002763"
     CDS             16780..17562
                     /gene="tatD"
                     /locus_tag="ACHKFK_002763"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO9053059.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAVTEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQELAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(17559..18047)
                     /gene="rfaH"
                     /locus_tag="ACHKFK_002764"
     CDS             complement(17559..18047)
                     /gene="rfaH"
                     /locus_tag="ACHKFK_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO9053060.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPSTPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            18214..19707
                     /gene="ubiD"
                     /locus_tag="ACHKFK_002765"
     CDS             18214..19707
                     /gene="ubiD"
                     /locus_tag="ACHKFK_002765"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO9053061.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            19753..20454
                     /gene="fre"
                     /locus_tag="ACHKFK_002766"
     CDS             19753..20454
                     /gene="fre"
                     /locus_tag="ACHKFK_002766"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO9053062.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(20727..21890)
                     /gene="fadA"
                     /locus_tag="ACHKFK_002767"
     CDS             complement(20727..21890)
                     /gene="fadA"
                     /locus_tag="ACHKFK_002767"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO9053063.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRHVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(21900..24089)
                     /gene="fadB"
                     /locus_tag="ACHKFK_002768"
     CDS             complement(21900..24089)
                     /gene="fadB"
                     /locus_tag="ACHKFK_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO9053064.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDTPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAILRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVIMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSHAKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            24279..25610
                     /gene="pepQ"
                     /locus_tag="ACHKFK_002769"
     CDS             24279..25610
                     /gene="pepQ"
                     /locus_tag="ACHKFK_002769"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO9053065.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPNSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            25610..26224
                     /locus_tag="ACHKFK_002770"
     CDS             25610..26224
                     /locus_tag="ACHKFK_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO9053066.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITLLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            26263..27714
                     /gene="trkH"
                     /locus_tag="ACHKFK_002771"
     CDS             26263..27714
                     /gene="trkH"
                     /locus_tag="ACHKFK_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO9053067.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFSLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            27726..28271
                     /gene="hemG"
                     /locus_tag="ACHKFK_002772"
     CDS             27726..28271
                     /gene="hemG"
                     /locus_tag="ACHKFK_002772"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO9053068.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQADVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         6639 a         7200 c         7582 g         6866 t
ORIGIN      
        1 aaaaaatgaa ggtgtgcagt gaacgtaatg attaacagac cggttaattt tcatgaatta
       61 cgtcaactgc acaaaaataa tttaaacggg tgctctaatt aaagcaagac ttatattttt
      121 atcattgtat tgtcaattaa atacaaatcg tgtcattaat tgtgggtttc ttaaatattc
      181 tatgcaccac tgacaggctt tgcccatacc atcggaacga gcggcaagaa ataacttggt
      241 ggcgtgttta tgttcgcgca ttggcttttt aagcaattta ccgctgttca ataacggcaa
      301 tgccagatga tgtggaatat aaccaatacc gacgttttgt tctattaagg cgattgccgt
      361 agcgaagtca ggaacaaaaa tgggtttttg tccttccaac aaccaggctt gcatcggggg
      421 gaagttaacg gaggtatcgc ggatacaaac cgctggataa tgacgcaatt cactgttttc
      481 taatggatgc cgcttcgctg ccagtgggtg gagtgggctg acgacaaaat cccattccat
      541 ttgtccgacc ggttcgctaa tgatgccttc actgcttggt actgcatgag gagcaccgta
      601 gaccagatca gcacgcttac tgtacagcgc atcccagcag ccgttgtata cctcaagatc
      661 taccgtcagt gttgttgatg tgaattgccg ttcaaaatct cgaataaatg ccaccagcaa
      721 atcaccagga ataatattgt ttaccgcaat tgtcagttca cgctcaatac catcgtgaat
      781 cagcactgtg ttgcgcttta atgcttccag gtcaccaaga atagttttgc tatgttcgat
      841 gaagtaactt cccgctggcg ataattcaat atagcgtccc ttacgaatga acagttcaac
      901 gcccaggctc tcttccgctt tcttgatagt gtaactgatg gcggaaggga ctttatttaa
      961 ctgctcggct gcggtggtaa tgctctggtg tctggcgacc agatgaataa cacgaagcgt
     1021 ttcgtctgag atgaacataa ggctccctgg tcagtggtaa agtggctgaa tctctccaga
     1081 ttcagccgta atcattgcct gagtataccc aggaattagc ggtgttgact tgcccgactg
     1141 agaatcgcat acgcgataca tgccaccaga atcgagtcta ccgaagggat cgtcgtcagg
     1201 gtgaataagc cttttacggt cataaagcca accgctgcgc ctatgatcca tgctgcgaat
     1261 gtcagcactt taatctgtga ctcctgggct aacactgact cttgatagcc attacggcga
     1321 taaaggaaaa agtcagcgat ataaattccg gcaacgggtg gcgtggcgat acccaggaac
     1381 agcagaaacg gaataaacca cgccataata tccatactgc ctacgatggc ggaaaggata
     1441 cccagcgcca cggtaatctg ccatttggga aagcgggtaa gcagtgtgga aaccaccagc
     1501 gtgccctgga acatattgcc cgcgttaccg gtgacgcagg cgaaaatcaa caacagcgcc
     1561 gcaggcagaa ccgcaccgaa taccgccatc gcacccagta aagatccctg accactaatg
     1621 gcgctcggcg ttgcacccgc ccagtagagc agagggtagg caaccagaaa cgtcacgcct
     1681 gcggcaatca gcgcatgttt gcggttatgc acaaaactac cgaaatcagg cagtgtggca
     1741 accagcacaa taatggtgcc aacaacggtg gaaacggcga cacctgtatt catctccaca
     1801 atcgccgctg gtactgccac ttcggtatgg gttgccacat agaggatgta acacagcacc
     1861 agcgcgataa ccggcacggc aacctgggca atttttccca gaacggcaaa gccaaatgcc
     1921 gtagaggcga caaaaatgac acagccagcc gctactaaca gtgccagtgg aacttccagt
     1981 ccatgttgag ccagtaagtc atgtaccgaa tgaccaaaca tattggcggt aacggcaatc
     2041 cagccgaaca gacttatcgc catcaaaatg ttaatggcga tggccccttt ttcgccaaac
     2101 gaatatttca caatgccata cgttggcagg cgggcttttt ctcccacgct aatggtgatg
     2161 gctgaaagta aggtcagaat gacgccgcct aacaatacga cgctaattaa actgctgctg
     2221 gaaagttgat tgccgaggct tgaggatgac aacagtacag gtgtgacggc aatacccagc
     2281 aaaatcatgg caatacgcaa tcctgatgcc gtattcagat tctcttcttt tttacgcata
     2341 gtatgttccc tttataaatg cgcgttacac agtatgaaaa tgcccggtgt atttgcgcca
     2401 gctacccagt tctgtaattt cccgttggcc ttcggtcagc cagttttctg ccagtacgcc
     2461 cagcacttcg ctaccatcgg caagtttgat tttgccaatt gccagcccag cgggttcgct
     2521 cattaacaga gcggcaaaac tggccagcgg taattgccaa atttctacgt cgacgtgtgt
     2581 tcctccttca ttcacgcgaa tcattcccgg atggcgatca ttaatactcc acaaccggta
     2641 atgggcgtca gttcggtctt cacggacaaa aataccgccc gctttttgca tatttgggtt
     2701 gagttccagg ccgcgcatca gcgttccgtt aacggctaaa agcgttgtag tcattgtgtt
     2761 ctccagagaa ttaaagggtt atccatgtac cgctctgatg cgccagggct gcttttatgg
     2821 tttgcggtga agtaatcagg ccgctggcct gtttgccttg ttgctggctg gcattgatga
     2881 aatcaacaat ggcttccact ttgggttgca tactgccgac accaaactga ccttcccgca
     2941 acagcgtttg cgcttcggca acgctgatag cgtcgagcca ctgttgttgt ggtgtaccaa
     3001 agttaatcgc tactttttct acaccggtgg ggatcaccag cagatctgcg cctaactgac
     3061 tggcgagcag ggcggaggcc agatctttat cgatcacggc ttccacacct ttcagttgtt
     3121 gcccgtctcg caccaccgga ataccgccgc cgccgcaggc aataaccaga tatccctggc
     3181 gcaccaggtg agcgatggtg tcgtgctcaa taatttccag tggtgcagga gagggaactg
     3241 tacgccgcca accacgcccg gcgtcctcca tcagcgtcca gccgagttgt tgttggcgtt
     3301 gttgggccgt tgcttcatcg agaaacgcgc caattggttt gctggggctg gcgaaagcat
     3361 cgtcatgtgg gctgactcgc gtttatgtca ccagggcaat taccggtttg tttatgccac
     3421 ggcgagccaa ttcgttatgc agcgctttct ggaacatgta gccaattgca ccttgtgtat
     3481 cacccacggc gtaatcaagt ggaaccggag aaacttcgtt actggcgagt tcagagcggc
     3541 gcagaataaa gcccacctgc gggccattac cgtgggttag caccaggtcc catccggctt
     3601 caaccatatc aacgatatgt tgcacgcttt ccatcactgc aacatattga tcgggaatac
     3661 tattgcgttg ctcgtcctga atgagcgcat tgccgccaac cgcgacgaca gccagtggct
     3721 taaccatgat ggttctcctt tacgacgatc tccgccagtg cacgtacagc ggcgacaaaa
     3781 caggccatcg gcgcagtggt gatcccggca ccaatttgcc cgacccccgc ttgtttgtgg
     3841 gcgatacccg tattgatgac cggtaaaatg ccgcgatcgg tcactttacg cgcatcaatc
     3901 cctgctgcgg tgggggcaaa attgagggcc ggaagtgtaa atgcggggtt accgccgagg
     3961 gtgatggctt gcatccgacg gctgttattg gtggcatcgg caggggtgcc tcccacaaat
     4021 ttgacgatcg ccggagacga cgccatcgcg aagccaccca cacctgcggt ttcggtaatt
     4081 gcgctatcac ccaggtcagc agcagcgtcg tcaacgccaa atccggggaa gaacagacct
     4141 tcaacggcat tggcaggagc ctggaaccag cgatcgccag tactggaaag ccgaatacca
     4201 aaattcaccc cgttacgcgc catcaccgta accattgaac tgaacggtac attggcggcg
     4261 gcatccatca ttgctttaca ggcagccatc gacagattga ggaagaagtg atcgttaccg
     4321 gtgataaatg ccacgacgcg ctggatatgt tcctgtggaa gagagcaggc cagcaatgcc
     4381 gggagcaggc gcttaatcag caaaccggta gcggcagcat tgcggttatg aacttcatcc
     4441 cccatatgca gcgcctgcgc cattaatggc ttgagttcca gctcgccgtg ttgtgcgatt
     4501 gctgctttca ttgccggcgc cagttcatcg cgcatccagg ccagacgatt gagaacttca
     4561 tcgttattgg cgccaaaacg gagaacctta cccagacctt cattgaaatt gctaaaagta
     4621 cggtggccgt tggttttgtt ttctaccacc cataacggca tcgacgggct gatgatgcct
     4681 gccattgggc caaccgcgtg ataatgatga caaggggcaa ggtcgatttc gcctgcgttt
     4741 atttggtttt cagcgtcttg ctggctggtg gcccaacctt cgtacagcat ggcaccaata
     4801 atcgcccctt ttaccggacc acacatctct tcccaggcaa tcggcgggcc agagtgcaaa
     4861 atacgttttt gttcagccat tgctggaatg gcctctttag cgagcatgac atccaccagc
     4921 acgggctgtg cttcgagata acgtgtcatg gcgatttggt tggcattttc taccagggga
     4981 tgacgtagca acgatgccag atccaatcca gcgtcaatat cgccttgtgc tggtggctgc
     5041 cagttgagtg caattgcgct tcccccggca tgttgaatat tgtcagcaaa actgtgtagt
     5101 cccgcgttca caaccttcaa aggctgatta aataaggtat tcatgcgttt agcccctttt
     5161 tctgcaactg agtctgtgca acggtgctgg cccatagcgc cgcctgtgca ttgctgctgg
     5221 cgatgatgac ccctgcattt tgtagtgcgc ttatctgctg actgcgtatt tgcggatcag
     5281 cttccgttcc gcaaacgtgc gcaatcagca acggtacgtt attcatgtcg atgtgtgaaa
     5341 gttggtccag taattcgctg gcaggcgtcg tggaagcgcc ataaccaagt accaggtcga
     5401 ataacacgac ggcagtgtgt gagtcgttga gttcgttgag cagacgttga ttgcgcagag
     5461 tcgggtcgat cattggatgc ggtttgccac gggtgaaatc atcgtcgccc atatcgatca
     5521 acgtgtgatc ttcgctttgc cagatatttg ccagagcacg attacccgca actggcgtat
     5581 tggaagctgc gctgaatccc ttttgttggc agataagttg cgcttcgtag cagaaggttc
     5641 ctccggcgaa aacaccgcgt atcgcctggc gatgggctgg caggcgctgg caggcgtttt
     5701 gtaacattgt gagatcgtcg cagggtacat cggtttcgac agccggaagt ggctgaacat
     5761 tcagtaacgc cacagcgatg tcggcggcgc tggcaagtgt ggtggccgcg gtgatattgg
     5821 gacgtgccag atcatgcgga ttcgcaccga ggaaattcac caccacgggt tttccacaag
     5881 cctcggcgcg ctccagaata gtctgggcaa cgatcggtga aggcggtttt gaaatcagca
     5941 caatcacgcg agtttcatcg tcttgcgcca gcgcatctaa agcgaagagc atcgatattc
     6001 caccaatctc ttcactgaga tcatgtccac cggttcccaa cgcctgagaa atacccgctc
     6061 cgagctggtc gattcggcaa gtcacttcct gtagcccggt gcctgacgcg ccaataacac
     6121 caattgctcc gcgtttcacc acattagcga agcctaacgg aatgccgtta acaatggcgg
     6181 ttccacagtc aggtcccatt acgatgcgct gacgttctcg ggcaagcgtt ttgatggatt
     6241 tttcctgccc gatactgacg ttatcgctga acatcatcac gttcattccg aggtttaacg
     6301 ctttaattgc ttcggcggcg gcgtagtctc ccggtacaga aatgagtgcc aggttggctt
     6361 cgggttcggc ttccagtgcc atttccaggc tgaccttttc cggtgtttta atgccactat
     6421 ccgtttcatc atcgggtttg ctggtcagac gttgctgggc cagtgccagt gcttcattgc
     6481 aaatatcttc ttcacccatg actgcaatca ctaaatcatt gggactggcg ttgatttcgt
     6541 tacccagacc cgcatcgcgc aattgttcga gattattggg cgttcccatg acgaccgagg
     6601 cctgttgaat accgggcaac ttgctgattt gcgcggaaat ctgcatcaga gaaactgagt
     6661 cctgataaag attactgaaa actttatatt taactttcat gctgttacgc ctgtttcatt
     6721 ccgcccgata gcaaaattcg ggcaaagcat ccccgtgtag ccccataaac gggctacggg
     6781 agaaaaggtt gaaatcaatt catggtgttc agggttcgaa agccatgcag cattcctgca
     6841 aggcaatcta ctcctgacgt tgcgccaaat tgcatgacct gttctgcaca ggatgcgatt
     6901 gtcattgccg atgcgctccc aaccagttgt gcgagtaact ggcaaatcgg ttctgaaaaa
     6961 tatccctgaa gcgcacgctc cagataatga cgactgatat ccgtggtgtt gtgcgcatgt
     7021 tgatcaattg cctgctgcaa ccggtattga tgatcggcaa gtggtgcagg gagttgccat
     7081 aaccagagcg ctgccagata gcccaacaaa tagtcatctc catcgggcgt taagccttta
     7141 ccaaagccga tcagttgtgg cacctggcgt tccagcgcat ctggatgttc cataattgct
     7201 aatccctggt gcgtgaggct atccggcagg tgcaatgtgc tttgaacgtt atgttccaga
     7261 caaaataaaa gccgttgcga tgccatcgtt gggtagtgct ggaaaaccaa agggaatgcc
     7321 tcaaatgtca gcattctccc cacaaatggc gactgccaga gcgttgcgtt atcgagcgcc
     7381 acacaaaatc gaggtgtccg cagcaagcct ggggttaact gaatgggatc tgaatgggca
     7441 atctctcttc gccagtccca acattcaggt agccagactc tggcagcatc agggagattc
     7501 agatacgttt gcgcacacaa cagagtgatg aatgttcccg cgtctgaata cagatttacc
     7561 gcatgtttaa aagcgctatg cagacgtaaa tcttgcgttt cattctgcgg gaaaagatat
     7621 cccaacgacg atgcgcaaag cgtaacctgt accatcggcg ttagccttcc agacctgcaa
     7681 taatggcttt ggaatcagta acccaaccga aaatggcacc ttgtgctttg atcatctcga
     7741 gggcgtattt ctggaattcc gggaaatagg agccgacaca atcttccggg ataatgcatt
     7801 catagccacg atcatttgct tcgcgcacgg tggtggttac gcaaacttcg gttgttacac
     7861 cacaaactat cagcgtcttg ataccgtggt tttgcaaaat cagatgtaag tcggtttgat
     7921 aaaacgcgcc tttgccaggc ttatcaatca ccggttcgcc tgccaccggg tacagttcag
     7981 gaatgatgtc atgacctgct tcacctctga ccagaatgcg tcccatcggt cctggttcac
     8041 caataaaggt ttttccgccg cgcgtcagtt tggctggcgg gcagtcgctt aagtctgcac
     8101 gatggccttc acgagtatga ataaccataa ttcctttttg acgtgcggct gccagcacct
     8161 ctgtacatgg tgcgatagcc gtgcgtacca gtgaaacatc attgcccagt gcctcaccga
     8221 agccccctgc ttcgacaaag tcacgctgca tgtcgatcat gaccagtgca gttgtgcgcg
     8281 gatcaaaagg taattcaaag ggttgagcct gaaaaatgga ttgcgtcatg gtaaatctcc
     8341 tgtctgatgt ttaaacgatc cagttgttct gagaacgagt attcaggaga aaagcggcac
     8401 aaaacaggaa aaaagtttgc cgcacagcaa cgaaattttt gcattcagca ggtggtgtga
     8461 tgacgaatgt gaagggagtc aaagagtggc atacagcaac aaattttttg atgctgtatg
     8521 gttttgagat taaaaaaatc gttgaaattg tgtgagttta atcatcagat ttagcgacta
     8581 tggcgttact ttttaacacc atactgtgta aaccattcca acattcgctg ccagccgtct
     8641 ttcgcagatt cggcatgata gctcgggcga taatcggcgt tgaacgcatg ccctgcatcc
     8701 gggtacacga taatctctgc tttcgcgttt gctgcccgca gcgcccggcg catcgtttcc
     8761 acgctctctt gcggaatgct gttatcctgg ccgccatata agccgagaac cggcgcgtta
     8821 agatcggttg cgatatcaac aggttgtttc ggtgaattca gcgacttatc gcccgtcagt
     8881 ttgccgtacc acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaggtg
     8941 atacgtccac cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg
     9001 gaagcccaac tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct
     9061 accagaccgc taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag
     9121 taaagttcag gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg
     9181 atatgttcat gcacgccaaa aatttcctgc actacaatga ccactggcag taggccatcg
     9241 ctttgctttg gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg
     9301 aagcccgcca caatcgcgtc gtccggagtc tgaacgatgg tcgaagcgag aggcgatgca
     9361 gcaggtgcaa atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa
     9421 tgttgcgcaa tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat
     9481 cttttatgca gtcctgcaga atgaagagtg atttatgtga tttacatcac ttttggtggg
     9541 taaatttatg caacgcattt tcgtcatggt gatgagtatc acgaaaaaat gttaaaccct
     9601 tcggtaaagt gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt
     9661 ccaagtctga tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg
     9721 ccatcgtccc tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg
     9781 ttaagctggc atctcaccgc gaattcacca cctggcgtgc agagctggat ggtaaacctg
     9841 ttatcgtctg ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg
     9901 cacagctggg cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata
     9961 ttaatgtggg tgatgtcctg gttaccacgg cgtctgttcg tctggatggc gcgagcctgc
    10021 acttcgcacc gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg
    10081 aagctgcgaa atccattggc gcgacgactc acgttggcgt gacagcttct tctgatacct
    10141 tctacccagg tcaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag
    10201 gttctatgga agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaaccc
    10261 tgctgaccat gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggg gttatcgtta
    10321 accgcaccca gcaagagatc ccgaatgctg agacgatgaa acaaaccgaa agccatgcgg
    10381 tgaaaatcgt ggtagaagcg gcgcgtcgtc tgctgtaatt atcttctctt gtctgaaggc
    10441 cgacgcgttc ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg
    10501 gacgtttgta cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt
    10561 ggatttctca atcatggttt acgcagttat tgcattggtg ggtgtggcaa ttggctggct
    10621 gtttgccagt tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat
    10681 ggtcgcggag ttaagcgcgg caaaacaaca aattactcaa agcgagcact ggcgcgctga
    10741 gtgcgagtta ctcaataacg aagtgcgcag cctgcaaagt attaacacct ctctggaggc
    10801 cgatctgcgt gaagtgacca cgcggatgga agctgcacag caacatgctg acgataaaat
    10861 tcgccagatg attaacagcg agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg
    10921 tatttttgag cacagcaatc gccgggttga tgagcaaaac cgtcagagtc tgaacagcct
    10981 gttgtcgccg ctacgtgaac aactggacgg tttccgccgt caggttcagg acagcttcgg
    11041 taaagaagca caggaacgtc ataccctgac ccacgaaatt cgcaatctcc agcagctcaa
    11101 cgcgcaaatg gctcaggagg cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac
    11161 ccagggcaac tggggcgagg tggtattgac gcgggtgctg gaggcttccg gtctgcgtga
    11221 agggtatgaa tatgaaactc aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc
    11281 ggatgtcatc gtgcggttgc cgcagggaaa agatgtggtg atcgacgcca aaatgacgct
    11341 ggtcgcctat gaacgctatt tcaacgccga agatgactac acccgcgaaa gcgcgctaca
    11401 ggaacatatc gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca
    11461 gctgccgggg ctaagaaccc tggattacgt gctgatgttt attcccgttg aacccgcttt
    11521 tttactggcg cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa acaatatcat
    11581 gctggttagc ccgactacgc tgctggtggc gctgcgcact atcgccaacc tgtggcgtta
    11641 tgagcatcaa agccgcaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa
    11701 gatgcgtttg ttcgtcgatg acatgtcagc gattggtcaa agtctcgaca aagcgcagga
    11761 taactaccgc caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc
    11821 agaagcgttt cgcggtttag gggtagaaat taaacgcgag attaatcctg atttggctga
    11881 acaggcggtg agccaggatg aagagtatcg actgcggtcg gttccggagc agccgaatga
    11941 tgaagcttat caacgcgatg atgaatataa tcagcagtcg cgctagccca tttggggtag
    12001 ttaagccggg tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt
    12061 tttgatgagc aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc
    12121 agaccgtcgc gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat
    12181 caaaatacga tgtcatgaat gatttgatgt cattcggtat tcatcgtttg tggaagcgat
    12241 tcacgattga ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca
    12301 ccggcgacct gacagcgaaa ttctctcgcc tggtcggaga aactggcaaa gtggtccttg
    12361 ctgatatcaa tgaatccatg ctcaaaatgg gccgcgagaa gctgcgtaat atcggtgtga
    12421 ttggcaacgt tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct
    12481 ttgattgcat caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc
    12541 gttcaatgta tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc
    12601 caattatcga gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta
    12661 ttggctcact ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta
    12721 tgcatcccga tcaggatacc ctgaaaacca tgatgcagga tgccggattc gaaagtgtcg
    12781 actactacaa tctgacggca ggggttgtgg cgctgcatcg tggttataag ttctgacagg
    12841 agaccggaaa tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc
    12901 ttcctgtatc gctcacccgc gctgaaaacg gcccgctcgc gtctgttggg caaagtgctg
    12961 cgcgtggagg taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtc
    13021 gatgtactgg gcgaatgggc aggcgatgct gactgcaccg ttatcgctta cgccagtgtg
    13081 ttgccgaaac ttcgcgatcg ccagcagctt gccgcactga ttcgcagtgg tgagctggaa
    13141 gtgcagggtg atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc
    13201 gaccctgcgg aactgctggc cccttatacc ggtgatatcg ccgctgaagg tatcagcaaa
    13261 gccctgcgcg gaggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg
    13321 gcggaagcca ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt
    13381 gcggaagaga cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa
    13441 ctggaggcta aatgacgcca ggtgaagtac ggcgcctata tttcatcatt cgcacttttt
    13501 taagctacgg acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat
    13561 ggcgatactc attattctgg atgccaaatc ggcataaaga caaaccttta ggtgagcgac
    13621 tacgactggc cctgcaagaa ctgggaccgg tatggatcaa gttcgggcag atgttatcaa
    13681 cccgccgcga tctttttccg ccgcatattg ccgatcagct ggcgttattg caggacaaag
    13741 tcgctccgtt tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc
    13801 cggtagaagc gtggtttgac gattttgaaa tcaagccgct ggcttctgct tctatcgccc
    13861 aggttcatac cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gttatccgcc
    13921 cggatatttt gccggtcatt aaagcggatc tgaaacttat ttaccgtctg gctcgctggg
    13981 tgccgcgttt gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg
    14041 aaaagacctt gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc
    14101 gacgcaattt tgaagacagc ccgatgctct acatcccgga agtttaccct gactattgta
    14161 gtgaagggat gatggtgatg gagcgtattt acggcattcc ggtgtctgat gttgcgacgc
    14221 tggagaaaaa cggcaccaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca
    14281 ctcaggtctt tcgcgacagc tttttccatg ctgatatgca ccctggcaac atcttcgtaa
    14341 gctatgaaca cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc
    14401 taaacaaaga agataaacgc tatctggcgg aaaactttat cgccttcttt aatcgcgact
    14461 atcgcaaagt ggcagagcta cacgtcgatt ctggttgggt gccaccagat accaacgttg
    14521 aagagttcga atttgccatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg
    14581 aaatttcgtt tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg
    14641 aagtgcagcc gcaactggtg ttactccaga aaaccctgct ctacgtcgaa ggggtaggac
    14701 gccagcttta tccgcaactc gatttatgga aaacggcgaa gcctttcctg gagtcgtgga
    14761 ttaaagatca ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct
    14821 gggtcgaaaa aatgccagaa ctgcccgaac tggtttacga cagtttgcgc cagggcaagt
    14881 atttacagca tagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg
    14941 gacaatcgcg ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt
    15001 tggtcagccg acctgaatgg gggttgatgc ccggctggtt aatggcaggt ggtctgatcg
    15061 cctggtttgt cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt
    15121 aacgtataat gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta
    15181 tcagtatttg gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa
    15241 agctcggctc catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga
    15301 gcgatgatga accaaagcag gataaaacca gccaggatgc tgattttact gcgaaaacta
    15361 tcgccgataa gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc
    15421 acgataaaga gcaggtgtaa tctgtgtttg atatcggttt tagcgaactg ctattggtgt
    15481 tcatcatcgg cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag
    15541 cgggctggat tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg
    15601 agttaaaact ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta
    15661 acctgacgcc cgaactgaaa gcgtcgatgg atgaattacg ccaggctgcg gagtcgatga
    15721 agcgttccta cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata
    15781 acccggtggt gaaagacaat gaaactgcgc atgaaggcgt aacgcctgct gctgcacaaa
    15841 cgcaggccag ttcgccggaa cagaagccag aaactacgcc agagccggtg gtaaaacctg
    15901 ctgcggacgc tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa
    15961 catgtctgta gaagatactc aaccgcttat cacgcatctg attgagctgc gtaagcgtct
    16021 gctgaactgc attatctcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga
    16081 catctatcac ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat
    16141 cgccaccgac gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct
    16201 gattctgtca gcgccggtga ttctctatca ggtgtgggcg tttatcgccc cagcgctgta
    16261 taagcatgaa cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tgttttatat
    16321 cggcatggcg ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac
    16381 cgcgccggaa ggggtacagg tatccaccga catcgcgagc tatttaagct tcgttatggc
    16441 gctgtttatg gcgtttggtg tctcctttga agtgccggtg gcaattgtgc tgctgtgctg
    16501 gatggggatt acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc
    16561 attcgttgtc gggatgttgc tgacgccgcc ggatgtcttc tcgcaaacgc tgttggcgat
    16621 ccctatgtac tgcctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg
    16681 gcgaaaccgg gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat
    16741 tcaaccgccc gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat
    16801 ttgaccagct cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg
    16861 ggagttaatg ggctactcat caccggtacc aatctgcgtg aaagccagca ggcgcaaaag
    16921 ctggcgcgtc agtattcgtc ctgttggtca acggcgggcg tacatcctca cgacagcagc
    16981 cagtggcaag ctgtgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg
    17041 gcgattggtg aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa
    17101 ctcgcttttg ttgcccagct acgcattgcc gcagaattaa acatgccggt atttatgcac
    17161 tgtcgcgatg cccacgagcg gtttatgaca ttgctggagc cgtggctgga taaactgcct
    17221 ggtgcggttc ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt
    17281 ggaatttata tcggcattac cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg
    17341 gaattgttgc cgttgattcc ggcggagaaa ttgctgatcg aaactgatgc gccgtatctg
    17401 ctccctcgcg atctcacgcc aaagccatca tcccggcgca acgagccagc ccatctgccc
    17461 catattttgc aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc
    17521 acggatgcta atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg
    17581 ttcttcacac tgtgcttaat ctctttatta ataagattaa gcaatagcat ggagcgagcc
    17641 tcgccgtctg gctcggtgaa aatggcctga aaaccttcga atgcgccttc agtaataatc
    17701 actttatcac caggatacgg cgtcgatgga tcgacaatat ctttcggttt atacactgat
    17761 aactgatgaa tcaccgccga tgggactatc gctggtgacg cgccaaagcg cacgaagtgg
    17821 ctgacaccgc gggtcgcgtt gatagtcgtg gtatgaatca cttccggatc gaactccaca
    17881 aacaagtagt tggggaacaa tggctcactg actgcagtac gtttgccacg cacgattttt
    17941 tccagggtga tcatcggtgc caggcaattc acagcctgtc tttcgaggtg ttcctgggca
    18001 cgttgaagtt gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc
    18061 cgtttaatcg gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt
    18121 ttgcgttata ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg caaagatgaa
    18181 cgccgtataa tgggcgcaga ttaagaggct acaatggacg ccatgaaata taacgattta
    18241 cgcgacttct tgacgctgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg
    18301 gatccgcacc tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg tgggcctgcg
    18361 ctgttgttcg aaaaccctaa aggctactca atgccggtgc tgtgcaacct gttcggtacg
    18421 ccaaagcgcg tggcgatggg catggggcag gaagatgttt cggcgctgcg tgaagttggt
    18481 aaattattgg cgtttctgaa agagccggag ccgccaaaag gtttccgtga cctgtttgat
    18541 aaactgccgc agtttaagca agtattgaac atgccgacaa agcgactgcg tggtgcaccc
    18601 tgccaacaaa aaatcgtctc tggcgatgac gtcgatctca atcgcattcc cattatgacc
    18661 tgctggccgg aagatgccgc gccgctgatt acctgggggc tcaccgtaac gcgcggcccg
    18721 cataaagagc ggcagaatct gggcatttat cgccagcagc taattggtaa aaacaaactg
    18781 attatgcgct ggctgtcgca tcgcggcggc gcgttggatt atcaggagtg gtgtgcggcg
    18841 catccgggcg aacgtttccc ggtttctgtg gcgctgggtg ccgatcctgc cacgattctc
    18901 ggtgcagtca cccccgttcc ggatacgctt tcagagtatg cgtttgccgg attgctgcgc
    18961 ggcaccaaga ccgaagtggt gaagtgtatc tccaatgacc ttgaagtgcc cgccagtgcg
    19021 gagattgtgc tggaagggta tatcgaacaa ggcgaaactg cgccggaagg gccgtatggc
    19081 gaccacaccg gttactataa cgaagtcgat agtttcccgg tatttaccgt gacgcatatt
    19141 acccagcgtg aagatgcgat ttatcattcc acctataccg ggcgtccgcc agatgaacct
    19201 gcggtactgg gtgtagcact gaacgaagtg ttcgtgccga ttctgcaaaa acagttcccg
    19261 gaaattgtcg atttttacct gccgccggaa ggctgttctt atcgtctggc ggtagtgacg
    19321 atcaaaaaac agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta
    19381 cgccagttta tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac
    19441 tggaacgatg tgatttgggc gattaccacc cgtatggacc cggcgcggga tactgttctg
    19501 gtagaaaata cgcctattga ttatctggat tttgcctcgc ctgtctccgg gctgggttca
    19561 aaaatggggc tggatgccac gaataaatgg ccgggggaaa cccagcgtga atggggacgt
    19621 cccatcaaaa aagatccaga tgttgtcgcg catattgacg ccatttggga tgaactggct
    19681 atttttaaca acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga
    19741 cagagaaagc gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat
    19801 accgtatatc gtgtccgcat cgtgccagac gcggcctttt cttttcgtgc tggtcagtat
    19861 ttgatggtag tgatggatga gcgcgacaaa cgtccgttct caatggcttc gacgccagat
    19921 gaaaaagggt ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca
    19981 gtcatggacc gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg
    20041 tggctgcgcg atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct
    20101 tatgcccgct cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt
    20161 tactggggcg ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg
    20221 ttgaagcatc ctggtctaca agtggtgccg gtggttgaac aaccggaagc gggctggcgt
    20281 gggcgtactg gcaccgtgtt aacggcggta ttgcaggatc atggtacgct ggcagagcat
    20341 gatatctata ttgccggacg ttttgagatg gcgaaaattg cccgtgacct gttttgcagc
    20401 gagcgtaatg cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata
    20461 aaaaaacccg cccctgacag gcgggaagaa cggcaactaa aactttattc agtggcatta
    20521 ctctacgttg catccggcaa acagtgcccg atgcaacgct gccgcgtctt atcgggccta
    20581 caaaagttct gaaccgtggg ccggataagg cgttcacgcc gtatccggca tacagtgccc
    20641 gatgcgacgc tgccgcgtct tatcgggcct acaaaagttc tgaaccgtag accggataag
    20701 gcgttcacgc cgcatccggc aagtggttaa acccgctcaa acaccgttgc aatgccctga
    20761 cccagaccga tacacatcgt cgccagacca aactggacgt ctttgtgttc catcagattc
    20821 agcagcgtgg tgctgatacg cgcaccggaa caacccagcg gatgacccag cgcgatcgcg
    20881 ccaccgttga ggttgatctt ctcgtcaatc tgctccatta gcccgagatc tttaatacac
    20941 ggcaggatct gcgcggcaaa ggcttcgttc atctcgaata cgccgatatc gctggcagaa
    21001 agccccgctt ttttcagcgc cagcttcgag gccggaaccg gaccataacc cataatagat
    21061 gggtcacaac ccacaaccgc catcgaacgc acgcgggcgc gcggcttaag acctaattca
    21121 cgggcgcggc tttcactcat caccagcatg gcagctgcgc catcggaaag cgcggaagat
    21181 gtacccgccg ttaccgtacc gctgacggga tcaaacgccg gacgcagtgt ggcgagggct
    21241 tccacggtgg tttccgggcg aatcacttcg tcgtaattaa actgcttcaa tacgccgtcg
    21301 gcatcgtgac caccggtcgg gatgatttca tttttaaatg cgcctgactg cgtggcggcc
    21361 cacgcgcggg cgtgtgaccg cgcggcaaag gcatcctgca tttcacggct gataccatgc
    21421 atacgcgcca gcatttctgc cgttaagccc atcatgcccg ccgctttggc gacattacgg
    21481 ctaaggccgg ggtgaaaatc gacgccgtga ctcattggca catggcccat atgctccacg
    21541 ccgccaacca gacatgcctg cgcatcgccg gtcatgatca ttcgtgctgc gtcatgcagt
    21601 gcctgcatgg atgaaccaca caagcgatta acggtaaccg ccgggacaga gtgcggcact
    21661 tcagccaaca gtgccgcgtt acgggcgatg ttaaaaccct gctccagtgt ttgctgcaca
    21721 caaccccagt aaatatcgtc gagggccgct gcttccagcg ccgggttacg cgccagcagg
    21781 ctacgcatta aatgagcgga gagatcttcc gcacgcacgt gacgaaaagc accgcccttc
    21841 gaacggccca tcggggtgcg aattgcatcg acaatgacaa cctgttccat tgtgactcct
    21901 taagccgttt tcaggtcgcc aaccggacgg gctggctcaa ccgggggata gtacggttcg
    21961 ttatgacgtg ctttattacg cagaccttcc ggtacttcat acagcgggcc gaggtgctga
    22021 tattgctgtg ccatatcgag gtattttgcg ctgccgaggg tgtccagcca gcggaacgcg
    22081 ccgccgtgga acggagggaa gcccaggcca tagaccagcg ccatatccgc ttctgccgga
    22141 gtggcgataa tgccctcttc cagacagcgg accacttcgt tgaccatcgg gatcatcatg
    22201 cgggcgataa tctcttcttc gctgaaatcg cgcttcgcgt ggctgacctc tgccagcagg
    22261 tcgtcaacga cggcgtcttc ttctttcttc ggcttgcctt tactgtcttc tttataacgc
    22321 cagaaaccga ggccgttctt ctgaccaaaa cggttggcat caaacagcgc atcgatggca
    22381 tcacggtaat ctttctgcat ccgctgcggg aagcctgctg ccatcacggc ctgggcgtgg
    22441 tgcgcggtat caatccccac gacatccagc agatatgccg gacccatcgg ccagccaaac
    22501 tgtttttcca tcactttgtc gatcttgcgg aaatccgcgc cgtcgcgcag caactggctg
    22561 aatccggcga agtaggggaa cagtacacgg ttaacgaaga agccggggca gtcgttgacc
    22621 acaatcggcg tcttgcccat cttgctcgcc catgcgacga ctttcgcgat ggtttcgtcg
    22681 gagcttttct cgccgcgaat aatttccacc aacggcatcc ggtgcaccgg gttaaagaag
    22741 tgcatcccgc agaagttttc cggacgttcc agcgcgttgg ccagttcgct gataggaatg
    22801 gttgaagtgt tagacgccag cacggtatcc gggcgtactt tctgctcggt ttctgccagc
    22861 acggcttttt tcactttcgg gttttcaaca accgcttcta ccacaacatc cacgcggtca
    22921 aatccggcgt agtcgagcgt tgggtggatt gtggagatca cgccagccag tttcagacca
    22981 tcgatcttgc cgcgttcaag ctgcttgttc agcagtttcg cggcttcggt catgccgagg
    23041 gttaacgact tgtcgttgat atctttcatg ataaccggca cacctttcca cgcagactgg
    23101 taagcgatgc cgccgcccat aatgcctgcg cccagcacag cggcctgttt cggcgtttca
    23161 acgtctttgg tgagtttctt cgctttgcct tttacatatt gatcgttaag gaatatgccg
    23221 accagtgcgc gagcttcgtt ggtatgcgcc agcgggacaa aacttttgtt ttccaggttt
    23281 agggcttctt cacgaccaaa acgggccgca gcttcaatgg tttttactgc agtgatgggg
    23341 gccggataat gtttccccgc cgtttgcgcg accatccctt tagcgatggt gaagctcatg
    23401 gtggcttcaa tcttgctcag ttttagcggt tccagcttcg gctgacgttt tgctttccag
    23461 tcgaggtcgc cgttaatggc ctgacgtaaa atcgccttcg cgccttcaac cagtttttct
    23521 gctttgacta cgccatccac cagaccgatt ttcagcgcct gatccgcgcc gacatcttta
    23581 ccggcggcaa tgatttccag agcactgtca gcgcccagca tacgtggcat acgtacagaa
    23641 ccgccaaagc ccggcatgat gcccagtttg gtttccggca gaccgatgcg cagatccggc
    23701 gtcgccagac gataatcggt cgccagcacg cattcgcagc caccgcccag cgcatagccg
    23761 ttgacggcag caatggtcgg caccggcaga tcttccaggc gattaaacac gctattggca
    23821 aagtgcagcc actgacttaa ctgttcttca ggaacgagga acagggacaa aaattcggtg
    23881 atatcagcac cgacgataaa ggctgctttg ttcgaacgta gcagcagtcc ttttagatct
    23941 gattgctgtt ccagcacgcc gatggcctcg ccgaggctgg cgacggtcgc agtgtcgagt
    24001 ttattaactg aacctggggt atcaaatacc agttcggcaa tgccatcttc cagccagtca
    24061 aggtaaaggg tgtcgccttt gtaaagcatg tcagtctcct gaatccgcaa ggtgatctgg
    24121 tcgtaccaga tgcatcgaag tgtgtatttt gtgttaaaaa tatgcaaata aaatattaag
    24181 gaaatgccga tctgatcacg ctcggcagaa atcacgctct ggatgaacga tgtgctaaga
    24241 tgcggagact taaggtcaaa aaaacagaag ggtaaaaaat ggaatcactg gcctcgctct
    24301 ataaaaatca tatagctacc ttacaggaac ggactcgcga tgcgctggcg cgcttcaagc
    24361 tggatgcgtt acttattcac tccggcgaac tgttcaacgt ttttctcgac gatcatccct
    24421 atccgtttaa agtgaacccg caattcaaag cgtgggtgcc ggtaactcag gtgccaaact
    24481 gctggctgct ggtggatggc gtgaataagc cgaaactgtg gttttatctg ccggttgatt
    24541 actggcacaa cgtcgaaccg ctgccgaact ccttctggac tgaagatgta gaagtgatcg
    24601 cgctgccaaa agccgatggc attggtagtc tgttgcctgc tgcgcgcggc aatatcggtt
    24661 atatcggtcc ggtgccagaa cgtgcgctgc aactgggtat tgaggccagc aatatcaacc
    24721 cgaaaggggt gatcgactac ctgcattact atcgctcctt caaaaccgag tacgaactgg
    24781 cctgtatgcg tgaagcgcag aaaatggcgg tcaacggtca tcgcgcggca gaagaagcgt
    24841 tccgttctgg catgagcgag ttcgatatca acattgccta tctgaccgcg accggtcatc
    24901 gtgataccga cgtaccttat agcaacattg tggcgcttaa cgaacacgct gcggtgttgc
    24961 attacaccaa actggaccac caggcaccgg aagagatgcg cagctttctg ctggatgccg
    25021 gggccgaata taacggctat gcggctgatc taacccgtac ctggtcggca aaaagcgaca
    25081 acgactacgc acagctggtg aaagacgtaa atgatgagca actggcgctg atcgccacca
    25141 tgaaagcagg cgtcagctat gtggattacc atatccagtt ccatcagcgc atcgccaaac
    25201 tgctgcgtaa acatcaaatc atcaccgata tgagtgaaga agcgatggtc gaaaacgatc
    25261 tcaccggacc gtttatgccg cacggtatcg gccatccgct gggcctgcag gtgcatgacg
    25321 tagccggttt tatgcaggat gatagcggta cgcacctcgc ggcaccggca aaatatccgt
    25381 acctgcgctg cacccgtatt ctccagccgg gcatggtgtt aaccatcgaa ccgggtatct
    25441 acttcatcga atcgcttctg gcaccgtggc gtgaagggca gttcagcaaa cacttcaact
    25501 ggcagaaaat tgaagcactg aaaccgttcg gcggcattcg tatcgaagac aacgtggtga
    25561 ttcacgaaaa caacgtggaa aacatgaccc gggatctgaa actggcgtga tggaaagctg
    25621 gttaattcct gcggcaccgg tcacggtcgt tgaagagatc aaaaagagcc gcttcattac
    25681 gctgttggcg cataccgatg gcgttgaggc ggcaaaagcg tttgttgaat cggtgcgggc
    25741 agaacacccc gatgcccggc accattgcgt ggcgtgggtg gcgggtgcgc cggatgattc
    25801 tcaacagctg ggtttctctg acgacgggga gccggcggga acggcaggta aaccgatgct
    25861 cgcccaacta atgggcagcg gcgtcgggga aattaccgct gtggtggtgc gctactacgg
    25921 cggcatattg cttggcaccg gtgggttagt gaaagcgtat ggcggcggcg tgaatcaggc
    25981 gctgcgccag ctaacgaccc aacgcaagac accattaacc gaatatactt tgcaatgtga
    26041 atatagtcag ttaaccggca ttgaagcgtt gctggggcag tgtgacggca aaattattaa
    26101 cagtgattat caggcattcg ttctgctgcg ggtggcgctt ccggcggcga aagtggctga
    26161 attttccgca aagctggcgg attttagccg tggttcattg caattgttag cgattgaaga
    26221 ataatcccca cttcgttttg cagaactaag gaagcggcag agatgcattt tcgcgccatt
    26281 acccgaatcg ttggactact ggtcatctta ttttcaggga ccatgattat ccctgggctg
    26341 gtagcactca tctaccggga tggagcgggc cgcgctttta cccagacctt ttttgtcgcc
    26401 ctcgccattg gctctatgct ttggtggccg aaccgcaaag agaagggcga actgaaatcc
    26461 cgtgaggggt ttctgatagt ggtgctgttc tggaccgtgc tgggtagcgt cggtgcgctc
    26521 ccttttatct tctcggaaag cccgaacctc acgattaccg atgcgttttt tgaatctttc
    26581 tctggcctga ccaccacggg ggccactacg ctggtggggc tggattcgct ccctcatgcc
    26641 atcctctttt atcgccagat gctgcaatgg tttggcggta tggggatcat cgtgctggcg
    26701 gttgcgatac tgcctatcct cggcgtgggt gggatgcagc tctatcgcgc agaaatgccc
    26761 ggcccgctga aagataacaa aatgcgcccg cgaattgcgg aaacagcgaa aaccctgtgg
    26821 ttgatttatg tcttgctgac cgtcgcctgt gcgctggcgt tgtggtttgc tggaatggat
    26881 gcctttgatg ccatcggcca tagctttgcg accatcgcta ttggcggctt ctcgacacat
    26941 gatgccagta tcggttattt cgacagcccg actattaaca ctatcattgc tatcttcctg
    27001 ctgatctctg gctgtaacta cggtctgcac ttttcactgt taagtgggcg tagtctgaag
    27061 gtttattggc gcgatccgga atttcgcatg tttatcggcg tacagttttc actggtggtt
    27121 atttgtacac tcgtactgtg gtttcataat gtctacagtt cggcgctgat gacaattaac
    27181 caggcgtttt tccaggtggt gtcgatggcg acaaccgccg ggtttacaac tgacagcatt
    27241 gcccgctggc cgctcttttt gccggtactg cttttatgtt cagcttttat cggcggttgc
    27301 gccgggtcaa cgggcggtgg cctgaaagtg atccgcatcc tgctgctgtt taagcagggg
    27361 aaccgtgagc tgaaacgact ggtgcatccg aacgccgtct atagcattaa gctggggaat
    27421 cgcgcactgc cggaacgtat cctcgaagcc gtttggggat ttttctccgc ctatgcattg
    27481 gtgtttattg tcagtatgct ggcgattatc gccacgggcg tggatgactt ttctgccttt
    27541 gcctcggttg ttgcaacatt gaataacctg gggccagggc ttggcgtggt tgctgataac
    27601 tttaccagta tgaacccggt ggctaaatgg atcctgattg ccaacatgct gtttggtcgt
    27661 ctcgaggtct ttacattgct ggtgctcttt accccgactt tctggcgtga atgatggagt
    27721 aatacgtgaa aacattaatt cttttctcaa caagggacgg acaaacgcgc gagattgcct
    27781 cctacctggc ttcggaactg aaagaactgg ggatccaggc ggatgtcgcc aatgtgcacc
    27841 gcattgaaga accacaatgg gaaaactatg accgtgtggt tattggcgct tctattcgct
    27901 atggtcacta ccattctgcg ttccaggaat ttgtcaaaaa gcatgcgacg cggttgaatt
    27961 cgatgccgag tgccttttac tccgtaaatc tggtggcgcg taaaccggag aagcgtactc
    28021 cacagaccaa cagctacgcg cggaagtttc tgatgaactc gcaatggcgt cccgatcgct
    28081 gtgccgtcat tgccggggcg ctgcgttacc cacgttatcg ctggtacgac cgttttatga
    28141 tcaagctgat tatgaagatg tcaggcggtg aaacggatac gcgcaaagaa gttgtctata
    28201 ccgattggga gcaggtggcg aatttcgccc gagaaatcgc ccatttaacc gacaaaccga
    28261 cgctgaaata agcataaaga ataaaaa
//
LOCUS       DAZGJT010000058        28229 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZGJT010000058 DAZGJT010000000
VERSION     DAZGJT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28229
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.058"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..206
                     /locus_tag="ACHKFK_002773"
     CDS             <1..206
                     /locus_tag="ACHKFK_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053069.1"
                     /translation="ADESMMQMKDVIWPAAEKEAYDKMKGMNATVVDVDKSAFKERVK
                     PLYDEFKAKDAQSAKNLELVESM"
     gene            248..730
                     /locus_tag="ACHKFK_002774"
     CDS             248..730
                     /locus_tag="ACHKFK_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9053070.1"
                     /translation="MRTLTHILNKILAGCCCIILAIMVFCVTWQVIARFIFNAPSTVL
                     DEFTQILFMWMILLGGVYTAGLKKHLAIDLLAQKLPAASVLTLDSFIQIIITVFAVIF
                     MIYGGNIVVEKAQHVGQISPVLKWPMDKVYWVMPVSGLILVWYSVMNIIDNYRKRNSH
                     "
     gene            741..2045
                     /locus_tag="ACHKFK_002775"
     CDS             741..2045
                     /locus_tag="ACHKFK_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9053071.1"
                     /translation="MEFEYIYPVLILFGSFAVMLAIGAPITFAIGLSSLLSIITALPP
                     DAAISVISQKMTVGLDGFTLLAIPFFVLAGNIMNTGGIARRLVNLAQALVGRLPGSLA
                     HCNILANTLFGAISGSAVASAAAVGGIMSPLQEKEGYDPAFSAAVNIASAPIGLMIPP
                     SNVLIVYSLASGGTSVAALFLAGYLPGILTAVALMFVAALYARRHHYPVAERINYRQF
                     LQVFRESIPSLMLIFIIIGGIIAGVFTPTEASAIAVIYSLALAMIYREITLKKLNDIL
                     LDSVVTSSIVLLLVGCSMGMSWAMTNADVPELINELITRVSDNKWVILFIINIILLIV
                     GTFMDITPAILIFTPIFLPIAQHLGIDPIHFGIIMVFNLTIGLCTPPVGTILFVGCSI
                     GKVSIDRAIKPLLPMFLALFVVMAIICYFPQLSLMLPGLFST"
     gene            complement(2116..3528)
                     /gene="ftsP"
                     /locus_tag="ACHKFK_002776"
     CDS             complement(2116..3528)
                     /gene="ftsP"
                     /locus_tag="ACHKFK_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO9053072.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3603..4340)
                     /gene="plsC"
                     /locus_tag="ACHKFK_002777"
                     /gene_synonym="parF"
     CDS             complement(3603..4340)
                     /gene="plsC"
                     /locus_tag="ACHKFK_002777"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO9053073.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4575..6833)
                     /gene="parC"
                     /locus_tag="ACHKFK_002778"
     CDS             complement(4575..6833)
                     /gene="parC"
                     /locus_tag="ACHKFK_002778"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO9053074.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDERRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLNIHVGKRKIKLRPEELQKVAGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(6971..8578)
                     /gene="ygiS"
                     /locus_tag="ACHKFK_002779"
     CDS             complement(6971..8578)
                     /gene="ygiS"
                     /locus_tag="ACHKFK_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9053075.1"
                     /translation="MYTQNLLWLVSLVSAAPLYAADVPANTPLAPQQVFRYNNHSDPG
                     TLDPQKVEENTAAQIVLDLFEGLVWMDGEGQVQPAQAERWEILDGGKRYIFHLRSGLQ
                     WSDGQPLTAEDFVLGWQRAVDPKTASPFAGYLAQAHINNAAAIVAGKADVTSLGVKAT
                     DDRTLEVTLEQPVPWFTTMLAWPTLFPVPHHVIAKHGDSWSKPENMVYNGAFVLDKWV
                     VNEKITARKNPKYRDAQHTVLQQVEYLALDNSITGYNRYRAGEVDLTWVPAQQIPAIE
                     KSLPGELRIIPRLNSEYYNFNLEKPPFNDVRVRRALYLTVDRQLIAQKVLGLRTPATT
                     LTPPEVKGFSATTFDELQKPMSERVAMAKVLLKQAGYDASHPLRFELFYNKYDLHEKT
                     AIALSSEWKKWLGAQVTLRTMEWKTYLDARRAGDFMLSRQSWDATYNDASSFLNTLKS
                     DSEENVGHWKNAQYDALLNQATQITDATKRNALYQQAEVIINQQAPLIPIYYQPLIKL
                     LKPYVGGFPLHNPQDYVYSKELYIKAH"
     gene            complement(8687..9169)
                     /gene="ygiV"
                     /locus_tag="ACHKFK_002780"
     CDS             complement(8687..9169)
                     /gene="ygiV"
                     /locus_tag="ACHKFK_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO9053076.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTERRLETDIYVPLA
                     "
     gene            complement(9222..9614)
                     /gene="ygiW"
                     /locus_tag="ACHKFK_002781"
     CDS             complement(9222..9614)
                     /gene="ygiW"
                     /locus_tag="ACHKFK_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO9053077.1"
                     /translation="MKKFAAVIAVMALCSTPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            9766..10425
                     /gene="qseB"
                     /locus_tag="ACHKFK_002782"
     CDS             9766..10425
                     /gene="qseB"
                     /locus_tag="ACHKFK_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO9053078.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            10422..11771
                     /gene="qseC"
                     /locus_tag="ACHKFK_002783"
     CDS             10422..11771
                     /gene="qseC"
                     /locus_tag="ACHKFK_002783"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO9053079.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLKANGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            11881..12462
                     /gene="mdaB"
                     /locus_tag="ACHKFK_002784"
     CDS             11881..12462
                     /gene="mdaB"
                     /locus_tag="ACHKFK_002784"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO9053080.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIIRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            12493..12807
                     /gene="ygiN"
                     /locus_tag="ACHKFK_002785"
     CDS             12493..12807
                     /gene="ygiN"
                     /locus_tag="ACHKFK_002785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO9053081.1"
                     /translation="MITVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GL"
     gene            complement(12852..13739)
                     /locus_tag="ACHKFK_002786"
     CDS             complement(12852..13739)
                     /locus_tag="ACHKFK_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO9053082.1"
                     /translation="MIQKDKVRVDIFGERFRTRASQLTPGLRVVASYINEHREVVLEQ
                     TAMEIAATLNTSDATVIRAIQALGFAGLRDLKRTLEQWLGPALSSSEKMSTTVSNLTS
                     DVNTAIDFVLEGHLYTCNVLSEPENRHALAQAVALLVQARQVAIFGIGASGILADYTA
                     RLFNRIGLPATALNRTGIGLAEQLIALQRGDVLIMMAQKSAHREGLTTLREARRLGIP
                     VILLTNALDSRFSKDASIVIHVPRGDEKGKTPLHGTVLLCLEMIVWSVASAVPQRAVK
                     TIKRINDFHRGLKTGRKNG"
     gene            complement(13736..14683)
                     /locus_tag="ACHKFK_002787"
     CDS             complement(13736..14683)
                     /locus_tag="ACHKFK_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-siderophore ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9053083.1"
                     /translation="MRLFFSLLILLSFFSRATEPVQVFTDDLGRKVTVPAHPKRIVSL
                     HDLDITIPLIELGVPPVASHGRTRPDGSHFIRSGALLTGVDFDNSSIAFIGTADIDIE
                     AIVAAKPDLIITEPTRNTPIEQLEKIAPTVSIDHLKGGAPEIYRKLAELTGTQSQLAI
                     LERRYQAQINALKATLDSQKITVSVIQANQGKINVMHSYHSLGRVLRDAGFRFPPLIE
                     SIPEGGRMDVSAERLPELDADFVFATWRGDTGGKPQDELAAMEKVMPGWCQFLAACRS
                     GRYVLISREEAISNSFASLGLMAAQIQSQIAGRPLPEAK"
     gene            complement(14694..15731)
                     /locus_tag="ACHKFK_002788"
     CDS             complement(14694..15731)
                     /locus_tag="ACHKFK_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9053084.1"
                     /translation="MNVGLRPLRVGKFSTLVRPKNLLLLGGLFLLAVGILIFGLMQGS
                     FSVPASEVGRALFAPENVSTDARYIVQDIRLPRVIMALLCGAMLGMAGAAMQSIARNG
                     LADPGLIGVKEGCSVAVLWLIFQFPMLGMFWRPVAGLAGGLLVALIVIFCARDISRPR
                     FVLIGIGVSWFFAAGIGVFMTTADVRDVQTALMWLSGSLHAANWMLVGISACWMLPAA
                     LLLLFTARTADIALLGHQVATGLGVNSSRLALLRVAAPIILTAVCVSCVGNIGFVGLI
                     APHISRFILRGGQTTLLLGSAVSGALLVILADSIGRLAFLPLQLPAGIIISLIGGPFF
                     LLLLWQRRNSF"
     gene            complement(15740..16723)
                     /locus_tag="ACHKFK_002789"
     CDS             complement(15740..16723)
                     /locus_tag="ACHKFK_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9053085.1"
                     /translation="MMRTGLVTVIFLALLLVGCVVYPGIGARFIAPQTVLQAFLHFDP
                     QNFDHNVIVRLRLPRLAAALLTGASLGVAGALLQAVIRNPLGEPHILGLNAGAALAVV
                     AASALGLAFPVGRPLLASTGGALLFLLILLLSSAGRSGVTPMKVTLCGVALSAFVSSI
                     TAAILILDEQTLLAMRTWLAGDLAGQDWATLGTSAWFSLGGFVLALYLAPSLNMLALG
                     DRMAQGLGVSVLRTRTFTLLAIALLCGAAVSIAGPIGFVGLLVPQIVRRLVSADLRVL
                     LPLSACVGALLLLLADIIARTLFTPHELATGVMTALVGAPVFVIMATRMFK"
     gene            complement(16720..17529)
                     /locus_tag="ACHKFK_002790"
     CDS             complement(16720..17529)
                     /locus_tag="ACHKFK_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9053086.1"
                     /translation="MAHNAKGQGLILDNVSAGYHKKIIVDGVSFSVPTEKMTVLVGAN
                     GCGKSTLLSTIARILQPMGGSILLDGKAIHEQPTKALSRRLGILPQSPLLPEGLTVYE
                     LVSRGRFPWQNFIRQWSDADEQAVEEALKLTGTQEFAHLPVEKLSGGQRQRCWIAMVL
                     AQKTPYILLDEPTTWLDLRYQVEILELLHDLTRHHGRTVVVVLHDLNFAVNYGDTLIF
                     LRQGKVVRVLNEGEHCTPELVKAVFDVDVHASINPLTGKPFFMPFRGVEKV"
     gene            17903..20044
                     /locus_tag="ACHKFK_002791"
     CDS             17903..20044
                     /locus_tag="ACHKFK_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFO9053087.1"
                     /translation="MAMFTPSFSGLKGRALFSLLFAAPMIHATDSVTTKDGETITVTA
                     DANTATEATDGYQPLSTSTATLTDMPMLDIPQVVNTVSDQVLENQNATTLDEALYNVS
                     NVVQTNTLGGTQDAFVRRGFGANRDGSIMTNGLRTVLPRSFNAATERVEVLKGPASTL
                     YGILDPGGLINVVTKRPEKTFHGSVSATSSSFGGGTGQLDITGPIEGTQLAYRLTGEV
                     QDEDYWRNFGKERSTFIAPSLTWFGDNATVTMLYSHRDYKTPFDRGTIFDLTTKQPVN
                     VDRKIRFDEPFNITDGQSDLAQLNAEYHLNSQWTARFDYSYSQDKYSDNQARVTAYDA
                     TTGTLTRRVDATQGSTQRMHATRADLQGNVDIAGFYNEILGGVSYEYYGLLRTDMIRC
                     KKAKDFNIYNPVYGNTSKCTTVSASDSDQTIKQESYSAYAQDALYLTDNWIAVAGIRY
                     QYYTQYAGKGRPFNVNTDSRDEQWTPKLGLVYKLTPSVSLFANYSQTFMPQSSIASYI
                     GDLPPESSNAYEVGAKFELFDGITADIALFDIHKRNVLYTESIGDETIAKTAGRVRSR
                     GVEVDLAGALTENINIIASYGYTDAKVLEDPDYAGKPLPNVPRHTGSLFLTYDIHNMP
                     GNNTLTFGGGGHGVSRRSATNGADYYLPGYFVADAFAAYKMKLQYPVTLQLNVKNLFD
                     KTYYTSSIATNNLGNQIGDPREVQFTVKMEF"
     gene            complement(20108..22000)
                     /gene="parE"
                     /locus_tag="ACHKFK_002792"
     CDS             complement(20108..22000)
                     /gene="parE"
                     /locus_tag="ACHKFK_002792"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO9053088.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(22029..22610)
                     /gene="yqiA"
                     /locus_tag="ACHKFK_002793"
     CDS             complement(22029..22610)
                     /gene="yqiA"
                     /locus_tag="ACHKFK_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO9053089.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(22610..23437)
                     /gene="cpdA"
                     /locus_tag="ACHKFK_002794"
     CDS             complement(22610..23437)
                     /gene="cpdA"
                     /locus_tag="ACHKFK_002794"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO9053090.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFTHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADARFQPDTASEGY"
     gene            complement(23462..23884)
                     /gene="yqiB"
                     /locus_tag="ACHKFK_002795"
     CDS             complement(23462..23884)
                     /gene="yqiB"
                     /locus_tag="ACHKFK_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO9053091.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(23885..24514)
                     /gene="nudF"
                     /locus_tag="ACHKFK_002796"
     CDS             complement(23885..24514)
                     /gene="nudF"
                     /locus_tag="ACHKFK_002796"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO9053092.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIIVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            24719..26200
                     /gene="tolC"
                     /locus_tag="ACHKFK_002797"
     CDS             24719..26200
                     /gene="tolC"
                     /locus_tag="ACHKFK_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO9053093.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNTLLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            26348..27019
                     /gene="ygiB"
                     /locus_tag="ACHKFK_002798"
     CDS             26348..27019
                     /gene="ygiB"
                     /locus_tag="ACHKFK_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO9053094.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            27025..28185
                     /gene="ygiC"
                     /locus_tag="ACHKFK_002799"
     CDS             27025..28185
                     /gene="ygiC"
                     /locus_tag="ACHKFK_002799"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9053095.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGIGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIAAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
BASE COUNT         7147 a         7459 c         6915 g         6708 t
ORIGIN      
        1 cagccgatga gtcaatgatg cagatgaagg acgtcatttg gcctgcggct gaaaaagaag
       61 cctacgacaa aatgaaaggg atgaacgcaa cagttgttga tgttgataaa tccgctttca
      121 aagaacgcgt caaaccgtta tacgatgaat tcaaggcgaa agatgcacaa tcagccaaaa
      181 accttgagct agtagaaagt atgtaaccct aatgggcagg taattcctgc cttttcatgg
      241 aagacttatg cgaacactca ctcatattct taataagatc cttgccggat gttgctgcat
      301 tattctcgcc attatggtgt tctgcgtaac ctggcaagtt attgcacgtt ttatttttaa
      361 cgctcccagc actgtgcttg atgagttcac ccagatttta tttatgtgga tgattttgct
      421 tggcggtgta tatactgcgg gtttaaaaaa acatctggcc attgatttgt tggctcaaaa
      481 acttccggct gcatctgttc tgacgcttga cagttttatc caaattatta ttacggtctt
      541 tgcggttatt ttcatgatct atggcgggaa tattgttgtt gaaaaagcac aacatgttgg
      601 acaaatctct ccagtattaa aatggccaat ggataaagta tattgggtta tgcctgtcag
      661 tgggcttatt ctggtctggt attcagtaat gaatattatt gacaactatc gtaaacggaa
      721 ttctcattaa cgagggaaat atggaatttg aatatatcta ccccgtctta attttatttg
      781 gcagttttgc cgtcatgctg gcaataggtg cgccaattac ttttgcgatt ggtctttctt
      841 cgctgttatc tattattact gccttgccac ccgatgccgc catttctgtt atttcacaaa
      901 aaatgactgt agggctggat ggctttacgc tattagccat tcccttcttc gtgttagccg
      961 gaaacattat gaataccggt ggtatagcca gacgactggt taacctggcg caagcattag
     1021 ttgggcgtct tcctggctca ctggctcatt gtaatatcct cgcgaataca ctgtttggtg
     1081 cgatttcagg ttcagccgtt gcgtcagccg cagcggtagg tgggattatg tcaccactgc
     1141 aagaaaaaga gggatatgat ccggcgtttt cagcagcggt taatattgcc tctgccccca
     1201 ttggcctgat gatcccaccg agcaatgtgt tgattgttta ttccctcgcc agtggcggga
     1261 cttctgtcgc tgcgttgttc ttagctggat atctgccggg catcctcacc gctgttgcct
     1321 taatgtttgt agctgcactt tatgcacgac gtcaccatta cccagtggcc gaacgcatca
     1381 attaccgtca gtttttacag gtattccgcg aatcaattcc tagcctgatg cttattttta
     1441 tcattattgg cggtattatc gcaggggtat ttacgcccac agaagcatca gcaatagcgg
     1501 taatttatag tttagccctg gcgatgattt accgggaaat cactcttaag aagctcaatg
     1561 atattctgtt agattcggta gtaaccagtt caattgttct gttactggta ggctgttcaa
     1621 tgggaatgtc atgggccatg acgaatgctg atgttcctga gttgatcaac gaactgatta
     1681 cccgcgtttc ggataacaaa tgggtcattc tgtttattat caatatcatt ctgttgatcg
     1741 tcggtacctt tatggatatc acgccggcga tcctgatatt tacacctatt ttcctgccga
     1801 tcgcccagca tctggggatt gatccgattc acttcggcat aattatggtt ttcaacttaa
     1861 ccattggcct ttgcacacca cctgtcggca ccattctgtt tgtggggtgc agcattggta
     1921 aggtcagtat cgacagggca ataaaaccat tactgccgat gtttctggca ttgtttgtag
     1981 taatggcaat tatttgttat ttcccgcagc ttagtctgat gctgccagga ttattttcga
     2041 cctgatacac aatgaagact gcatactgag taattttcaa tgccccacca ctctgtgggg
     2101 cattgaattg gtccattacg gtaccggatt gaccaacagt tgcccaatcg agccacggtc
     2161 cgccatttcc agcgtctgac tgttgaagta gaacgggaag tgcgcccagg aaggctgacc
     2221 gaaataaaca agcagctcca cttgtccgtc aacccaaacg gtatctttcc agcctctgtc
     2281 ttccgggaac ggcatcgcgc cattcacgtt acggatctgg aacatcacgc cttcaatatg
     2341 gaacgcttgc ggctcgtccg cgcgcaccgt ccagcgttcc cacgttcctt gctgcgcagt
     2401 aacatcaata cggttgacgt cccacaactg cccattaatc cccgggtcat cacccagact
     2461 gatatcacga cttcgaatcg gcgaaccggc catgatttca gttggcagca agcgcatcgg
     2521 aagactgtct gtgaccagcg gcagaaggcc ggttgggcgc agcgttagca ccagcgtaga
     2581 aaccagaatg ctggatggct caaagaagcc acgaatacga tcaacaatgc tcgccgcttc
     2641 gccacaggta atcgacactt catcaccgtt gctcatatcc accagaatct cacgacgctc
     2701 gcccggtgcc agcgaaagtt gcttcaccga tacaggagcg ggcaggaatc cctgatcgcc
     2761 agaaatcaca tgtaacgggc gaccatcgct catctgtagt tgatagcgac gagagttcga
     2821 cgcgttcaac agacgcaagc gcacccagcc acgcgagact tcaacgtacg ggctttgtac
     2881 accgttaacc agcagcgtat caccaacaaa gccgccgctt cccggttcgt tatattctgg
     2941 cgtaccaaag ttatccagcc gtttatcctg aataatgacc ggaaaatcat ccacaccata
     3001 atggttgggg ataggcagcg atttgctgac ttcatcttcc accagccaca ttccggcaag
     3061 gccgttatag acctgctgag ccgtgcggtt gggagtattg gcgtgatacc acagagtagc
     3121 tgcgttctgg cgaatgggca gtacgggtgc ccagtcagcg tttggcgaca tcatccgtgc
     3181 cggaccgccc atcagcgggc caggtacctg tagcccggcc accgtcattg agacattttc
     3241 tgtcaggcgg ttgctgtaaa taagcttaac atcgtcgccc ttccagacgc ggatagtcgg
     3301 ccccaggtaa cgaccattga ttccccaaac cgacgcgcgt gtccctggcg taaatgacca
     3361 gtgcgcacgt tgtacagtca taaacagcgg ttgcccacgg cgagattcaa gcagcggcgg
     3421 aacgggtagc ggttgctgtt gcccggctgc gctggccttc aggggaacag cgcctgcaca
     3481 aagtgcaatc cccgatgcct gaatgaactg acgccgactg agtgacatat ttgctccatg
     3541 taaaactggc taaagtaata ccgtgcagga aagtgttccc cgcaaaaata cagattgccg
     3601 acttaaactt ttccggcggc ttcgcgttct gcgacttctt tatcgagctc ggcgattttt
     3661 tgttccatta tcgaacgaca atgggcagcc aactcgcgaa cctgatcttt gccatactga
     3721 ctgacgtcaa ttggcggcag catttcgaca atcaccagac cgttgtgtag ccgattaaga
     3781 ttaatcttat tcgaagttgt agagacgcac acgggtataa tcgggacgcc cgccgcaatt
     3841 gccgcgtgaa atgccccggt cttgaacggc agcaggccgc gaccacggct gcgggttcct
     3901 tccgggaaca tccagatgga aatacggcgt tttttgaagt gattcactac ttccgcaata
     3961 gtgccgtgag ctttagtgcg attgtttctg tcgatcaata agttgccggt taaccagtac
     4021 aactgcccga agaaggggat ccacagcaag ctttttttac ctaccgtcac tgtcggcggc
     4081 tgcacaatgt tcgatgccgt caccatgtca taattgttct ggtggttagc aatatagata
     4141 gcattgccgt agctttcagc gtctgcaggt ttgcggcact ccactttcag gccaaaaagc
     4201 ggcgcaagac ggccaaacat atgcccaaag gtggccacat gtttcgggtt acgcgggctg
     4261 aaaaggcagt aaatggagcc gaatacacag actaagatgc tgtaaatcac ggtaataata
     4321 agacgaaaga tatatagcat aacaccctct gaaggtctga gagactggca ttgtacgtca
     4381 cctgaaaatc aggttagggc attgttaaaa gccgctctct ggaaaagcgt attgttaatc
     4441 gcaacgcatg aataaacaac ggtttcaagg gaatttttat cgtttccggg ggttgaatac
     4501 gcggccggat gacgacttaa cgtttcatcc ggcgttcctt gcaagcggga ggaaacagcg
     4561 ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc
     4621 tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca
     4681 gcgactttct gtagctcttc cggacgcagt ttaattttgc gtttcccaac atgaatgttc
     4741 agcgtgcttt gcggcggcag aacgtacagt tgcgcaagac catcctcgcc acgcgcggct
     4801 tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatcg
     4861 cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat atcggaagca
     4921 tcttcaatca ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcttta
     4981 cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg
     5041 gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgctcca
     5101 ggcggcaacg ttaatttgcc ggtgagcggc tcgccctgac cacgcgccga cggcagcgta
     5161 atcgggtcga tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc
     5221 ttacctttca ccgccgcttt gaagctatcg cccgctttat aattcaggcc cggcgcgtcg
     5281 atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt
     5341 tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc
     5401 gaacgacgtt cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt cagcaggtta
     5461 ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagctca
     5521 ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg
     5581 aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt
     5641 tcatcttcat tacgaatgat ctcaatcact tcgtcgatat tgagaaacgc caccagcaaa
     5701 ccttcgagga tatgcaggcg cttgaggact ttctccagac gatagttcag tcggcggcgc
     5761 acggtatcgc ggcggaacac cagccattcg gagaggattt ccagcaggtt tttcaccgcc
     5821 ggacgaccat ccagaccgat catattgagg ttaatgcgat agctcttttc cagatcggtg
     5881 gtagcaaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc
     5941 accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc
     6001 tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc
     6061 ggcaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg catacgcact
     6121 gaaccacgtc cgttctcgta gattttacga atctcggcgc gcgaagtgat aatttccgct
     6181 tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc
     6241 tggtcgatta atgcgattgc cgcctgagcc acttcacgca ggttatgcgg tggaatatcg
     6301 gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgggca
     6361 ggcagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc
     6421 tgccccagct cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc
     6481 attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc atcaaccagc
     6541 ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca ggcactatcg
     6601 ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg
     6661 gcgctggcat tcaggcccag ctcagacatc gcatacacaa tgcggcgctg aacaggtttc
     6721 agaccatcac caataaacgg caacgcacgg tccatgatca cgtacatgga gtagtttaag
     6781 taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct
     6841 gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat
     6901 ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccgacggc
     6961 gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacataa tcctggggat
     7021 tatgcagagg aaaaccgcca acgtagggtt taagcagttt gattaacggc tgatagtaga
     7081 tgggaatcag cggtgcctgc tggttgatga tcacttctgc ctgctgatac aacgcattac
     7141 gctttgtcgc atcagtgatc tgcgtggcct ggtttagtaa ggcgtcatac tgcgcatttt
     7201 tccagtgacc gacgttttct tcactatcgc ttttgagcgt gttcaggaag ctggaggcat
     7261 cattgtacgt cgcatcccac gactgccgag acagcataaa atcaccggct cgtcgggcat
     7321 caagataggt tttccactcc attgtgcgca gcgtcacctg tgcacctagc cattttttcc
     7381 attcggaaga caacgctatc gcggtctttt catgcagatc gtacttgttg tagaacagct
     7441 caaagcgtag cggatgagag gcgtcgtatc ccgcctgttt cagcaagact tttgccatcg
     7501 cgacacgctc actcattggc ttttgcagtt catcgaacgt cgtcgcgcta aagcctttta
     7561 cctctggcgg cgtcagcgtg gttgcgggcg ttctcaaccc cagtaccttt tgcgcaataa
     7621 gctgtcgatc aaccgtaaga tatagcgccc gacgcacccg cacatcgtta aatggcggtt
     7681 tctcaaggtt aaagttgtaa tattcgctgt tcagacgcgg gataattcgt agctcgccag
     7741 gcagtgattt ttcaatggcg ggaatttgct gcgccggaac ccaggtgaga tcgacctctc
     7801 ccgcgcgata gcggttatag ccggtgatcg aattatccag cgccagatac tcaacctgtt
     7861 gcaatactgt atgttgcgca tcgcggtact ttggattttt gcgtgcagta atcttttcgt
     7921 taactaccca tttatcaagc acaaaggcac cgttgtaaac catgttctct ggcttactcc
     7981 agctatcgcc atgtttagcg atgacatgat gaggaaccgg gaacagcgtc ggccaggcga
     8041 gcatcgtcgt gaaccaagga actggctgct caagcgtaac ttcaagagta cgatcatccg
     8101 tcgctttgac acccagcgat gtaacatctg ctttacccgc aacaatagcc gcggcattgt
     8161 taatgtgcgc ctgtgccaga tagccagcaa aagggcttgc cgttttcggg tcaaccgcac
     8221 gttgccagcc gaggacaaaa tcctctgccg tcagaggctg accgtctgac cactgcaaac
     8281 cgctacgtag atggaaaata tagcgcttgc cgccgtcaag tatctcccag cgttcagcct
     8341 gagcgggctg cacctggcct tcaccgtcca tccataccag accttcaaac agatccagca
     8401 caatctgcgc ggcagtattc tcctctacct tttgcgggtc gagcgtgcct gggtcgctat
     8461 gattgttgta acgaaagact tgttgcgggg cgagcggtgt gttggcggga acgtcagcag
     8521 cgtagagagg agccgcactt accaggctga ccagccataa taaattttgc gtatacatgg
     8581 tattccttac cggagaaatt ataagcccga caacaatacg cctgtggcat tgtttgctca
     8641 aacttatcgc gagtgatttg gctcacactc cggtaaagat tccccatcac gctaacggta
     8701 cataaatatc cgtttccagt cgccgctctg tcatcccttc ggcaagattg gtgtagtgaa
     8761 acaggatcgg ggctttgcgc attttttcgc cgcttgcagg tagccagtgg cgaatgatgc
     8821 cccatatcgt gtgtgaaata tcgtccagct cgccaacatg gcgggcaaca gcataacgcc
     8881 caccggtaag ttcgccatta ctcacgccat aacgattatc ggggatcggt tcactaacgc
     8941 tgccgcagat atcaaagcga aacgcttccg gtgcggtggt ggctgggtcg tcccaggcga
     9001 cgccaaaagt ctggctttgg ttaacaggag aaagccccgt ctctttacgc cacatgataa
     9061 atttcgccac gctgtagttg agcaactcag ggctacagcg gtgcggcaac atcgccacag
     9121 ggattgatgg gaaatcgata atatttacat ccagtgtcag gtttgtcatt ttgcctccct
     9181 gcgaaattat tcccgggaac gcgtcccggg agcggtaaca attacggatt tactttgcgg
     9241 atctgtttga cgtcaatttc aacagagttc cagtctttat cgacttcccc ctgaatctca
     9301 accgtatctt tcggcgtcac cgtcacgccg ttccagcgtt tgtgatcgat atccacattg
     9361 atagtaccgc tggcatcttt gaacacgtag agatcgtcag agatgcgttc aacgatattg
     9421 ccgcgcaggg ttacccaggt gtcgtcacgc agggattttg cgctttctac ggtcgttacg
     9481 ctgccgttcg gcccctggaa tcctccggcc tgactttgcg ttgccgatgg gccagaaaaa
     9541 cctccctgct ctgctgccat aaccggtgta ctgcacaggg ccattactgc gattactgct
     9601 gcgaattttt tcatgtttat tactcccttt aatgtctgtt tccgagcatt taacaagata
     9661 gtccttaaca acttcttaag ggaaaaaaat aaaatttaat gctgtacaga gcgcgttaca
     9721 acacggttta ctggcagcaa atacggttat cgcagggatg aaaaaatgcg aattttactg
     9781 atagaagatg acatgctgat tggcgacggc atcaaaacgg gcctcagtaa aatgggtttt
     9841 agcgtcgact ggtttacaca aggtcgtcag ggaaaagagg cgctatatag cgcaccttat
     9901 gatgcggtga tcctggattt aaccttaccg ggcatggatg gtcgcgatat tttgcgcgaa
     9961 tggcgagaaa aaggtcagcg tgagccggta ttgatcctga ctgcgcgcga tgcgctggcg
    10021 gaacgtgtag aaggactgcg tctgggagct gacgattatc tgtgtaaacc ttttgcgttg
    10081 atagaagtcg ccgccaggct ggaagctctg atgcgccgaa ccaacggcca ggccagcaac
    10141 gagctgcgcc acggcaacgt catgctcgac cccggcaaac gtatcgccac gctggctggc
    10201 gaacccttaa cgctgaaacc aaaagaattt gccctgctgg aattactgat gcgtaacgct
    10261 ggtcgggtac tgccgcgcaa actgattgaa gagaaactgt atacctggga cgaagaggtc
    10321 accagtaatg ccgttgaagt gcatgtgcat catctgcgac gcaaactcgg cagtgatttt
    10381 attcgtaccg ttcatggtat tggctacaca ttaggtgaga aatgaaattt acccaacgtc
    10441 ttagtctgcg agtcaggctg acgctaatct ttttaattct ggcctcggtg acctggctgc
    10501 tttccagctt tgtcgcctgg aaacaaacaa cggataacgt cgatgagttg ttcgacaccc
    10561 aactgatgct gtttgccaaa cggttaagta cgctcgatct caacgaaatc aacgcggcgg
    10621 atcgcatggc acagacgcca aataaattaa aacacggtca tgttgatgac gatgctctga
    10681 cctttgccat ctttacccac gacggcagaa tggtccttaa cgatggcgat aacggagaag
    10741 atattcctta tagctatcag cgggaaggtt ttgctgacgg gcaactggtc ggtgaagacg
    10801 atccctggcg ttttgtctgg atgacctcac ctgatggcaa atatcgcatc gttgtgggtc
    10861 aggaatggga ataccgtgaa gacatggcgc tggcgattgt tgccggacaa ttgatcccgt
    10921 ggctggtcgc actgccgatt atgttaatca tcatgatggt actactgggt cgtgaactcg
    10981 cgccgctgaa caaactggcg ctggcactac gtatgcgtga cccagactcg gaaaaaccac
    11041 taaacgcgac gggcgtaccc agcgaagtgc gcccactggt tgagtcgcta aatcaactgt
    11101 tcgcccgcac acatgcgatg atggttcgtg aacgacgctt tacctccgac gcagctcacg
    11161 aacttcgtag cccgttaacg gcgctgaaag tgcaaaccga agttgctcag ctctctgacg
    11221 atgatccgca ggcgcggaaa aaagcgctgc tccaattaca ttccgggatc gatcgcgcta
    11281 ctcgtctggt tgatcaactg ctcacgctat cgcggctgga ctcactggat aaccttcagg
    11341 acgtcgcgga gatcccgctt gaagatctcc tgcaatcgtc ggtgatggat atttaccaca
    11401 cggcgcagca ggcgaaaatt gacgtgcgac tgacactcaa agccaacggc atcaaacgca
    11461 ccggacaacc gctattgcta agtttgttgg tgcgaaattt gctggataac gccgtgcgtt
    11521 acagtccaca aggtagcgtg gtagacgtca cgctgaatgc cgataatttc atcgtgaggg
    11581 ataacggccc tggtgtgaca ccagaggcac tggcgcgaat tggcgaacgc ttctatcgcc
    11641 cacccggaca aaccgctacc ggcagtggac tagggctatc gattgtccag cgaatcgcca
    11701 aattgcatga catgaatgtt gaatttggga atgcggaaca aggtggattt gaggcgaagg
    11761 taagctggta agggtaagac ttttgctaaa ttcgcaaaag actttgcaca ttttgctaat
    11821 ttcaccgtac cgctctgtga cgtactatag tcggcaaacg tctcaccttg aggtaaaaaa
    11881 atgagcaaca tcctgattat caacggcgcg aaaaaattcg cccactccaa tggtcaactg
    11941 aacgacaccc tgaccgaagt cgcggatggc acactgcgcg acctcgggca tgatgtccgc
    12001 attattcgcg ccgacagcga ctacgatgtc aaagcggaag tacaaaactt tctctgggct
    12061 gatgtggtga tctggcagat gcctggctgg tggatgggcg cgccgtggac agtgaaaaaa
    12121 tatattgatg atgtattcac cgaaggtcac gggactctgt atgccagcga tggtcgtacc
    12181 cgcaaagatc catcaaaaaa atacggttcc ggcggcctgg tacagggcaa aaaatatatg
    12241 ctttctctga cctggaacgc accaatggaa gccttcaccg aaaaagatca gttcttccac
    12301 ggcgttggtg ttgacggtgt gtacctgccg ttccataaag caaaccaatt ccttggcatg
    12361 gaaccgctgc cgacatttat cgctaatgac gtgataaaaa tgcctgatgt tccccgctat
    12421 actgaagaat atcgcaagca tcttgtggaa atttttggtt aactagagct ctggctttag
    12481 aaggagttaa ccatgattac agtaatcgca gaaatccgta ctcgtcctgg tcaacatcac
    12541 cgtcaggcag tgttggatca gtttgctaaa atcgttccaa ccgtactgaa agaagaaggt
    12601 tgccacggct atgcgccaat ggtggattgc gctgctggcg tgagtttcca gtctatggca
    12661 ccggattcta tcgtgatgat tgagcagtgg gaaagcatcg cgcatcttga agcgcatctg
    12721 caaaccccgc acatgaaagc atatagcgaa gccgtaaaag gtgacgtgct ggagatgaat
    12781 attcgtatcc tgcagccggg gctttgacat ttctaaaaag aggccggtgc tgctaccggt
    12841 ctcagcccag attagccatt ctttcttccc gttttcagtc ctcgatggaa atcgttaatt
    12901 cgcttaatgg ttttaaccgc acgctgcggc actgcggaag ctaccgacca gacaatcatt
    12961 tccagacaca acaataccgt accatgtagc ggcgttttcc ctttttcatc cccgcgcgga
    13021 acatgaatca cgatactggc gtctttgcta aaacgtgaat ccagcgcgtt tgtcagcaaa
    13081 ataacaggga tccccagccg cctcgcctca cgaagcgttg tcagcccttc ccggtgcgct
    13141 gatttttgcg ccatcatgat cagaacatcg ccacgttgaa gcgcgatcag ttgctcggca
    13201 aggccaatac ccgtacggtt tagtgccgtg gcaggcaaac ctatgcggtt gaatagtcgt
    13261 gcggtatagt ccgccagaat acccgaagcg ccaatgccaa aaattgcgac ttgccgtgcc
    13321 tggaccaaca acgctacagc ttgcgcaagc gcgtggcggt tttcaggctc tgacaggaca
    13381 ttacaggtat acaaatgccc ttcaagaaca aagtcgattg cggtgttaac atcgctcgtc
    13441 aagttactca ccgtcgtaga catcttttcg ctggaactaa gggctggacc aagccactgt
    13501 tccagagtgc gtttcagatc acgcagcccg gcaaacccca gggcctgtat agccctgata
    13561 actgtggcat cggaagtgtt caacgtggcc gcaatttcca tagccgtttg ctcgagtacc
    13621 acttcacgat gctcattaat gtagctggcg acaactctta acccaggcgt taactgactc
    13681 gcgcgagtgc gaaaacgctc accaaaaata tcaacccgca ctttatcttt ctggatcatt
    13741 tagcctccgg tagaggtcgc cctgcaattt gtgattgtat ctgcgcagcc atcagcccca
    13801 gagatgcaaa ggagttagaa attgcctctt cacgagaaat cagcacataa cgcccggaac
    13861 gacaggcagc cagaaactga caccatcccg gcatgacttt ttccattgct gccagctcat
    13921 cctgaggttt accaccggtg tcaccgcgcc aggttgcaaa aacaaaatcg gcatccagtt
    13981 caggtaaacg ctccgcgctc acatccattc gcccaccttc aggaatgctc tcaatcagcg
    14041 gcggaaaacg aaaacccgca tcgcgtaata cccgccccag cgaatgatag ctatgcatca
    14101 cattaatttt tccctggttt gcctgaatca ccgataccgt tattttttgg ctgtccagcg
    14161 tcgccttcag cgcattgatt tgtgcctgat agcggcgttc aagtatcgcc agctgcgact
    14221 gcgttcctgt aagttctgcc agcttgcgat agatttctgg cgcaccccct ttgagatgat
    14281 caatgctcac cgtgggcgca attttttcaa gttgttcaat gggcgtatta cgggtcggtt
    14341 ctgtgatgat caggtcaggc tttgccgcca caatcgcttc aatatcaata tcagcggtac
    14401 caataaaggc gatcgatgaa ttatcaaagt cgacacctgt caataacgcg ccagatcgaa
    14461 tgaaatggct accgtcaggc cgagttcgcc cgtggctggc gacaggaggt acaccgagct
    14521 caatcagagg aatagtgata tcaagatcgt gtaaagagac aattctcttt ggatgagcag
    14581 gaacagtgac tttgcgccct aaatcatcag taaatacctg aacaggttct gttgcacggg
    14641 aaaaaaatga caacagtatc agcagagaaa aaaataaacg catataactc ccgttaaaaa
    14701 ctgttcctgc gctgccagag cagtagcaaa aaaaatggtc caccaatcag tgagataatg
    14761 attccggcag gcagttgcaa aggcaaaaaa gccaaacgac caatgctatc cgccagaata
    14821 actaacaacg cccccgatac cgcgctcccc agcagtaatg tcgtctgccc gccacgtaga
    14881 ataaaacgtg atatatgggg tgctatcaat ccgacaaaac cgatgttacc aacacacgaa
    14941 acgcagacgg ctgtcaggat tatgggtgcg gcgacacgta acaacgccag acgagaacta
    15001 ttcaccccta acccggttgc aacctgatga ccaagcagag cgatatccgc agtcctggcg
    15061 gtgaacagca ataaaagagc ggctggcaac atccaacagg ctgaaatacc gactaacatc
    15121 cagttagctg catgcaggct tcccgataac cacatcaatg cagtttgtac atctcgaaca
    15181 tctgcagtgg tcataaatac accgataccg gcggcgaaaa accaggaaac accaatacca
    15241 atcagaacaa atcgcgggcg ggaaatatcg cgcgcacaaa aaatcacgat taaagccacc
    15301 aacagaccac cagcaagacc agccactgga cgccagaaca ttcccagcat tggaaactgg
    15361 aaaatcagcc acagcaccgc cacgctacat ccttctttga cgccgattaa accaggatcg
    15421 gcaaggccgt tacgcgcaat ggattgcatc gcggcccccg ccatacctaa catggccccg
    15481 catagcaatg ccatgatgac tctcggcaag cgaatatcct gcacaatata acgtgcgtct
    15541 gtgcttacgt tttctggagc aaacaacgct cgtcctactt ccgatgccgg aacagagaaa
    15601 gaaccctgca tcagaccgaa tatcagtatt ccaaccgcaa gcaggaacag cccaccaagc
    15661 aacagcagat ttttagggcg caccagtgtc gaaaacttac caacacgtag tgggcgtagc
    15721 ccgacgttca ttatccgact catttgaaca tcctcgtcgc cataatgaca aaaacaggcg
    15781 caccaactaa cgccgtcatg acaccggtcg ctaactcatg gggcgtgaaa agcgtccggg
    15841 caataatatc tgccagtaat aacaaaagcg cgccgacaca agctgaaaga gggagcagaa
    15901 cgcgcagatc cgctgatacc agacgccgga caatctgcgg taccaacagg ccgacaaaac
    15961 caatggggcc agcaatcgat acggctgcgc cacagagcaa cgcgatcgca agcagggtaa
    16021 atgttcgcgt gcggagtaca gagacgccca gcccctgcgc catgcgatcg cccagtgcca
    16081 acatgttaag ggagggtgca aggtaaaggg ctaacacaaa tccccccaga gaaaaccagg
    16141 cggacgtacc cagcgtcgcc caatcctgcc ccgctaaatc acctgctagc catgtccgca
    16201 tcgccagcag tgtttgttcg tcaagaatca ggattgcagc cgttattgag gacacgaacg
    16261 ccgatagcgc cacgccgcat aaagtaactt tcattggcgt tacccctgaa cgccctgcgg
    16321 aggaaagcag caatatcaac aggaacaata acgcgccccc cgtagatgcc agcagtgggc
    16381 gtccaaccgg aaaggccagt ccaagcgcac tggcggcaac gacagcaaga gcggctccgg
    16441 catttaagcc taaaatatgt ggctccccca atgggttacg aatgacagcc tgcaacaaag
    16501 caccagcaac accaagagaa gcaccggtaa gcaatgctgc cgctaaacgc ggcaaacgga
    16561 gtcttacaat tacgttgtga tcgaagtttt gcggatcaaa atgaagaaat gcctgcaaca
    16621 ccgtttgtgg tgcaataaat cgggcaccaa tccctggata taccacgcat cccaccagca
    16681 gtaaggcgag gaaaattaca gtaaccagcc ctgtgcgcat catacttttt ctacaccacg
    16741 aaacggcatg aagaacggct ttcccgtcag aggatttatt gatgcgtgaa catcaacgtc
    16801 aaagacagct tttaccagtt caggcgtgca atgctccccc tcattcaaca cacgcaccac
    16861 tttcccctgg cgtaaaaata tcaacgtatc gccataattg acggcaaaat tcagatcatg
    16921 caacaccacg acgactgtac gcccgtgatg acgcgttaaa tcatgtaata actcaagaat
    16981 ttcaacctga taacgtaaat caagccaggt tgtgggttca tccagcagga tataaggcgt
    17041 cttttgcgcc aacaccatcg caatccagca acgctggcgc tgcccaccag agagtttttc
    17101 aacaggtaaa tgtgcaaact cctgcgtacc ggttaatttc agggcctctt ccaccgcctg
    17161 ctcatcggcg tcgctccatt gacgtatgaa attttgccag ggaaaacgcc cacgcgacac
    17221 cagttcataa acggttaagc cctccggcaa taatggcgac tgcggcaaga tcccaagtct
    17281 acgggataac gctttcgttg gttgctcgtg aatcgctttg ccatcaagca atatcgaacc
    17341 gcccatgggt tgcaagatac gtgcaatggt gctgagcaac gtcgactttc cacagccgtt
    17401 tgccccaacc agaacggtca ttttttctgt gggaacggag aaagaaacac catcaacaat
    17461 gatcttcttg tgatacccgg cagaaacatt gtcgaggatc agcccctgcc cttttgcgtt
    17521 atgtgccacg tgtacgccaa cttaatcaaa gacaaacaaa aataaaactc attctccttt
    17581 gagagtttga cgccacaaaa tgtagtagtc aagcagttaa aagccacaca aaaacaaggt
    17641 aaataacagg taaactattt ttatacataa aaataaaaac attaaatatc aataaattaa
    17701 aagcaatcaa gttgtataaa cttttcataa aaacacatgt agcaattttt tgttatttct
    17761 catttactac tacattactg cgtgattgaa tgattctcaa ttacatatcc gatggcgaaa
    17821 taacaaacgg atagtgctta gtgagcaata agaaacaatt ttcactcgcg acaatccgcg
    17881 agtttttggg ataacagact aaatggctat gttcacacct tcattctcag gactcaaagg
    17941 tcgggcgctc ttttcactgc tttttgcggc accgatgatt catgcaacag actctgtaac
    18001 gaccaaagat ggcgaaacaa tcactgttac agcagatgca aataccgcaa ctgaggcaac
    18061 cgatggttat caacctctga gcacctccac ggcgacatta accgatatgc cgatgctgga
    18121 tatcccgcag gtggtcaata cggttagcga tcaggttctg gaaaaccaga atgcgacaac
    18181 gctggatgag gcgctttata acgtcagtaa cgtggtacag accaatacat taggcgggac
    18241 tcaggatgct tttgtacgcc gtgggtttgg cgcaaaccgg gatggctcca tcatgaccaa
    18301 cggtctgcga accgtacttc ctcgtagttt caacgccgca acagagcgtg tggaagtgct
    18361 aaaaggcccg gcctccacgc tgtatggcat tctcgatcct ggcggactga ttaacgtcgt
    18421 gaccaagcgc ccggaaaaaa cattccatgg ttcggtttca gccacctcct ccagttttgg
    18481 tggcggcact gggcaacttg atatcacagg tcccattgaa ggcactcagc tggcgtatcg
    18541 ccttaccggg gaagtgcagg atgaagatta ctggcgaaac ttcggtaaag agcgcagtac
    18601 atttattgcc ccgtcactca cctggtttgg tgataatgca acagtaacca tgctctattc
    18661 ccatcgggac tataaaactc cgttcgatcg tggaacgatt ttcgacctta cgacgaaaca
    18721 gcccgtaaac gttgatcgaa aaatacgttt tgacgaaccg tttaatatta cagatggtca
    18781 gtccgatctg gcgcaactca acgcagaata tcatctcaat agccagtgga cagcgcgctt
    18841 tgattacagc tacagccagg ataaatacag cgataatcag gcgcgtgtta ccgcgtatga
    18901 tgcaacgaca ggaacactga cacggcgtgt tgatgcaact cagggatcta cccagcgtat
    18961 gcatgctact cgtgcggatc tgcaagggaa tgttgatatt gccggattct ataatgagat
    19021 tctgggtggg gtgtcatatg aatattatgg tcttctgcgt acagatatga ttcgctgtaa
    19081 aaaagctaaa gatttcaata tatacaaccc tgtttatggt aataccagca aatgtacaac
    19141 ggtttcggcg tcggacagtg atcagacgat caaacaggag agctactcag cgtatgcaca
    19201 ggacgcactc tatctgaccg ataactggat tgccgtcgcc gggatccgat atcagtatta
    19261 cacgcagtat gcgggtaaag gccgtccttt taatgtcaat actgacagcc gcgatgaaca
    19321 atggactccc aaactggggt tggtctacaa actgacgcca tcggtatcct tatttgccaa
    19381 ttattcgcaa acatttatgc cgcagtcgtc aattgccagc tacattggcg atcttccacc
    19441 agaatcatct aatgcttacg aagtcggggc aaaattcgag ctattcgatg gtatcaccgc
    19501 agatattgcg ctgtttgata tccataaacg taatgtgttg tataccgaaa gtattggtga
    19561 tgaaaccatc gccaaaacgg caggccgtgt tcgttcaaga ggggtagaag tcgaccttgc
    19621 gggagcatta actgaaaaca ttaatatcat tgccagctac ggctataccg atgctaaggt
    19681 tctggaggat cctgattatg cagggaaacc attgccgaat gttcctcgtc ataccggttc
    19741 gctattcctg acctatgata ttcataacat gccaggcaat aatacgctga catttggcgg
    19801 tggtggacat ggtgtaagcc gtcgttcggc aaccaatggg gctgactatt atctgccagg
    19861 ctattttgtt gccgatgcct tcgccgcata caaaatgaaa ttgcagtatc cggtcacact
    19921 gcaattaaac gtcaaaaacc tgtttgataa aacgtattac acctcttcca tcgccacaaa
    19981 taatctgggc aaccagattg gcgatccgcg tgaagtgcaa ttcacggtga aaatggaatt
    20041 ttgatcaaaa aagcccggta tacacttgta taccgggcta tgttttattc ttctggcggg
    20101 aggattatca gacctcaatc tctgccatgt cgcctttctc ttgcaaccag ttgcggcgat
    20161 cttccgagcg tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt
    20221 catcatcgat agtcaactgc accagacggc gagtgttcgg atcaagcgtg gtttcgcgca
    20281 attgcatcgg gttcatttcc ccgagacctt taaaacgctg gacgtttggc ttgcctttct
    20341 tgcgttttaa ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg taataaacct
    20401 ctttcccgag gtcaatacgg tagagcggtg gcagtgcaac gtaaacgtga ccgtgtttca
    20461 ccaatgcgcg gaagtgtttt acaaacaaag cgcagagcag cgtggcaatg tgcagaccat
    20521 cggagtcagc atccgccagg atacagatct tgccgtaacg aagctggctc aaatcgtcgc
    20581 tgtcaggatc gataccgata gctaccgaaa tatcgtgcac ttcctgcgaa gccagcactt
    20641 cgtcggaaga gacttcccag gtgttaagga tcttaccttt cagtggcatg atcgcctgat
    20701 attcgcgatc gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc acaaggaata
    20761 gttcggtacg gttaagatcc tgcgcggtac agtcagccaa tttaccaggc agcgccgggc
    20821 cgctggttag ctttttacgc accacttttt tagccgcacg catacgacgc tgggcgctgg
    20881 aaatcgccat ctctgccaac agctccgccg cctgaacgtt ctggttcagc cacaggatga
    20941 aggcatcttt caccacgccc gaaacgaatg ccgcacactg acgcgaagag agacgttctt
    21001 tggtctgccc ggcaaactgc ggatcctgca tttttactga cagcacatag gcgcagcgat
    21061 cccagatatc ttccgccgac agctttacac cacgcggcag aatgttgcgg tattcacaga
    21121 actcacgcat cgcgtccagc aggccctgac gcaggccatt aacatgggta ccgccctgca
    21181 tcgttgggat caggttgacg tagctttcgg tcagcagttc accgccttcc ggcagccaca
    21241 gtagcgccca gtccaccgct tcagtatcgc cagcgaaatt accgataaac ggtttttctg
    21301 gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc attcagaccg tcctgatagc
    21361 accagcgctg ttcggtgttg ttgatctcat ctttaaaagt gatctcaacg cccgggcaca
    21421 gtaccgcttt ggctttcagc acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa
    21481 agaaggtttc atccggccag aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc
    21541 cgacaacctg taaatcttgc accttttcgc cattttcaaa ggcgatgtta tagatctgac
    21601 catcgcggcg tacgttaact tctacgcgct tcgacagggc gttaactacc gaaatcccca
    21661 cgccatgcag gccgccagag aactggtaat ttttgttaga gaatttaccg cccgcgtgca
    21721 gacggcaaag aatcagttca accgccggta ccccctcttc cgggtgaata tctaccggca
    21781 tcccgcgccc atcgtcaatc acttctaacg actggtcggc atgtaagatt acatccacgc
    21841 gttttgcgtg gcccgccagt gcttcatcga cactgttatc aataacttct tgccccaaat
    21901 ggttagggcg agtggtatcg gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg
    21961 tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt agcaattcga
    22021 gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa ataatcttcg
    22081 aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga agcgtagtac
    22141 gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag ccagatcaaa
    22201 tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg gcgtgactct
    22261 agcacatatt gctgcccggt gtaggggttc tcgttctgac cgagatagtc cgtcagcagt
    22321 tcaaacggac gcaccgccgg gttgaccacc actgcgggca gcataaaaca ttgcgacaac
    22381 caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt
    22441 tccaggacaa tagattccag cagttctgcc gcgtcagaag gatacggggg caactgcgga
    22501 atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa gctcgctttt
    22561 gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt
    22621 ctgaagcggt atcaggttgg aagcgtgcgt ccgccaggcg atgcacctcg gtggtcagcg
    22681 tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatggtgtcc agcgtaaagt
    22741 tagaacagtg cggcttaaac tgcacacagg tcgacggcgt tgccagcaag cggcgaccgt
    22801 tccagtcgag atccagctcc tgatgaatat gaccgcacag caagtatttg acgtgcgtaa
    22861 acttcgccag cacggtatcc agttcgcccg cgttacgcag gctgtgttga tcaagccaac
    22921 tacaacccgc aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat
    22981 cggccagttt acgttccagc cactcaagct gaaactcgct cagctcaccg tgcggcacgc
    23041 caaacacctg gctatccagc aacaggattt gccattgctc accaataaac acgcgcttcg
    23101 ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc
    23161 ccggcagcca gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg
    23221 ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct
    23281 ggtgtggccg aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt acccctaaca
    23341 gggcttcgtg cttttgtgca aacaggtgag tgtcggtaat ttgtaaaatc ctgactctgg
    23401 cctcaccagc cagaggaagg gttaacaggc tttccaaatg gtgtccttag gtttcacgac
    23461 gctaataaac cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac cagtccgcta
    23521 aaaactgatt aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta ggataatcat
    23581 accgcgcttt gaagcgaaaa atctgctggc ttgaacacac ttcagccacc atcgcgtcat
    23641 gatacagacg caccgtcatt gacggaaggc tccagtaact gatcgcgggc gcagtctgtt
    23701 ctattgtcac cagggtagtg tatcgggtcg attccacaat cgtcagccga tattgtgcgt
    23761 ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca
    23821 attgtgaaaa gttcatctcg cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct
    23881 tcatttatgc ccactcattt tttaacgctt gatgatgcag ctgcagccat tgcaaagcga
    23941 tgaccgacgc tgcgttgtcg attttcccct cttctaccca ctggtatgcc tgttcccggc
    24001 ttaccacatg aacgcgaata tcttcgtttt catcagccag accgtgaata ccgcttgcgg
    24061 tcgtggcgtc cacttcgccc acaataattg acgaacgctc actggtgccc cccgggcttg
    24121 ccaggaaact taacaccggt ttggtccgtt tgactatcag tcccgcctct tcaatcgctt
    24181 cgcgacgggc aacatcttcc acactttcac cctcttcaat catcccggca accatctcca
    24241 gtagccaggg ggtttcgctg gtgtcgtacg cggcaatccg aatctgctca atcagcacaa
    24301 cttcatcacg cactgggtca aagggtagca agactgcggc gtgaccgcgc tcaaaaattt
    24361 cccgccgtac ctcatgactc atttgcccgt tgaatagacg atgacgaaat ctataaagat
    24421 ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc
    24481 caaatgtaac gggcaggttg tctggcttaa gcattgttaa tgtcctggca ctaatagtga
    24541 attaaatgtg aatttcagcg acgtttgact gccgtttgag cagtcatgtg ttaaattgag
    24601 gcacattaac gccctatggc acgtaacgcc aaccttttgc ggtagcggct tctgctagaa
    24661 tccgcaataa ttttacagtt tgatcgcgct aaatactgct tcaccacaag gaatgcaaat
    24721 gaagaaattg ctccccattc ttatcggcct gagcctctct gggttcagtt cgttgagcca
    24781 ggccgagaac ctgatgcaag tttatcagca agcacgcctt agtaacccgg aattgcgtaa
    24841 gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca gtccattact
    24901 gccacagcta ggtttaggtg cagattacac ctatagcaac ggctaccgcg acgcgaacgg
    24961 catcaactct aacgcgacca gtgcgtccct gcagttaact caatccattt ttgatatgtc
    25021 gaaatggcgt gcgttaacgc tgcaggaaaa agcagctggg attcaggacg tcacgtatca
    25081 gaccgatcaa caaaccttga tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc
    25141 tattgacgtt ctttcctata cacaggcgca aaaagaagcg atctaccgtc aattagatca
    25201 aaccacccaa cgttttaacg tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc
    25261 acagtacgat accgtgctgg cgaacgaagt gaccgcacgt aataaccttg ataacgcggt
    25321 agagcagctg cgccagatca ccggtaacta ctatccggaa ctggcggcgc tgaatgtcga
    25381 aaactttaaa accgacaaac cacagccggt taacacgctg ctgaaagaag ccgaaaaacg
    25441 taacctgtcg ttgttacagg cgcgcttgag ccaggacctg gcgcgcgagc aaattcgcca
    25501 ggcgcaggac ggtcacttac cgactctgga tttgacggcc tctaccggaa tttctgacac
    25561 ctcttacagc ggttcaaaaa cccgtggtgc cgctggtacc cagtatgacg atagcaatat
    25621 gggccagaac aaagttggcc tgagcttctc gctgccgatt tatcagggcg gaatggttaa
    25681 ctcgcaggtg aaacaggccc agtacaactt tgttggtgcc agcgagcaac tggaaagcgc
    25741 gcatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg cttctatcag
    25801 tagcattaac gcctacaaac aagccgtagt ttccgctcaa agctcattag acgcgatgga
    25861 agcgggttac tcggttggta cgcgtaccat tgttgatgtg ttggatgcga ccaccacgct
    25921 gtacaacgct aagcaagagc tggcaaatgc gcgttataac tacctgatta accagttgaa
    25981 tattaagtca gctctgggta cgttgaacga gcaggatctg ctggcactga acaatgcgct
    26041 gagcaaaccg gtttccacta atccggaaaa cgttgccccg caaacgccgg aacagaatgc
    26101 tattgctgat ggttatgcgc ctgatagccc ggcaccagtc gttcagcaaa catccgcacg
    26161 cactaccacc agtaacggtc ataacccttt ccgtaactga taacgacaac ggggcttcgg
    26221 ccccgtctga acgtaaggca acgtaaagat acgggttatc tgccgcattc ttcccccttc
    26281 tcgcttcaat ttcgaccagc catcctctat tctgatgggt atttaccact ggtcccggaa
    26341 gacaaaaatg aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa actggagcgc
    26401 acgccatctg acaccagtcg ctctcgcggt tgccactgtt tttatgctgg ctggctgtga
    26461 aaagagtgat gaaacagtgt ctctctatca aaatgctgac gactgttcag ctgcaaaccc
    26521 aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac
    26581 tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa tttggtgaag gtcagtgcca
    26641 gcaggcacca gcccaggctg gcatggcacc agaaaaccag gcgcaggccc agcaatccag
    26701 cgggagtttc tggatgccgc tgatggccgg ttacatgatg gggcgtctga tgggcggcgg
    26761 cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac ccggccagcc cggcttacgg
    26821 taaatatacc gacgcgaccg gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac
    26881 cgtaccgaag acggcaatgg caccaaaacc ggcgaccact actactgtta cccgtggcgg
    26941 ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt agcgcaaccg gcacctcttc
    27001 tcgttcaatg ggtggctgat accgatggaa agagtcagta ttaccgagcg cccggactgg
    27061 cgcgaaaaag cccacgaata cggtttcaat tttcacacca tgtacggcga gccgtactgg
    27121 tgtgaagatg cttactacaa gttgaccctc gctcaggttg aaaagctgga agaagtcacc
    27181 gccgaactgc accagatgtg cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg
    27241 atgaccaaat tccgcatacc aaaacacacc tggagttttg tgcgccagtc atggctgacg
    27301 caccagccat cgctttattc gcgtcttgat ctggcatggg atggtattgg cgaacctaaa
    27361 cttctggaaa ataacgccga tacgccaacg tcactatacg aggcggcgtt ctttcagtgg
    27421 atctggctgg aagatcagct taacgccggt aacttgccgg agggcagcga ccagtttaac
    27481 agtttgcaag aaaagctgat cgatcgcttc gttgagctgc gtgaacagta tggcttccag
    27541 ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc gcggaaccat tcagtatttg
    27601 caggactgcg cgacggaagc tgaaatcgct accgagttcc tctacatcga cgatatcggg
    27661 ttaggtgaaa aaggtcagtt cacggattta caggatcagg tgatttccaa cctgttcaag
    27721 ctgtatccgt gggaatttat gttgcgtgag atgttctcca ccaagctgga ggatgcaggc
    27781 gtacgctggc tggaaccggc gtggaagagc attatctcca acaaagcgct tctaccgcta
    27841 ctgtgggaga tgttcccgaa tcacccgaac ctgctgcccg cttattttgc ggaagatgat
    27901 catccgcaaa tggaaaaata tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg
    27961 tcgatcattg agaacggcaa aaccattgca gcagcggaag gtccgtatgg cgaagaaggg
    28021 atgattgttc agcaattcca cccgttaccg aaattcggcg acagctatat gctgattggt
    28081 agctggctgg tgaacgatca acctgccgga attggcattc gtgaagaccg tgcattgatc
    28141 acccaggata tgtctcggtt ttatccgcat atttttgttg agtaatacag cttaatattg
    28201 ccccgataag cggggcaatc tcttataat
//
LOCUS       DAZGJT010000059        28003 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZGJT010000059 DAZGJT010000000
VERSION     DAZGJT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28003
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.059"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..698
                     /gene="bcsQ"
                     /locus_tag="ACHKFK_002800"
                     /pseudo
     CDS             1..698
                     /gene="bcsQ"
                     /locus_tag="ACHKFK_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            695..3313
                     /gene="bcsA"
                     /locus_tag="ACHKFK_002801"
     CDS             695..3313
                     /gene="bcsA"
                     /locus_tag="ACHKFK_002801"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO9053096.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLFHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIIL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPAQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            3324..5660
                     /gene="bcsB"
                     /locus_tag="ACHKFK_002802"
     CDS             3324..5660
                     /gene="bcsB"
                     /locus_tag="ACHKFK_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO9053097.1"
                     /translation="MKRKIFWICAVALGMSAFPSFMTQATPATQPLINAEPAVAAQAE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVELQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIVGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRLNPDNE"
     gene            5667..6773
                     /gene="bcsZ"
                     /locus_tag="ACHKFK_002803"
                     /pseudo
     CDS             5667..6773
                     /gene="bcsZ"
                     /locus_tag="ACHKFK_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
     gene            6755..10228
                     /gene="bcsC"
                     /locus_tag="ACHKFK_002804"
     CDS             6755..10228
                     /gene="bcsC"
                     /locus_tag="ACHKFK_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO9053098.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVIAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADTPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVQALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVVNLEKALALDP
                     HNSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAYIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRGQWNSNIQE
                     LVNRLQNDQVLETANRLRENGKEAEAEAMLRQQPPSSRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDKAAARSQLAKLPAIDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEALAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDTADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            10310..12298
                     /gene="hmsP"
                     /locus_tag="ACHKFK_002805"
     CDS             10310..12298
                     /gene="hmsP"
                     /locus_tag="ACHKFK_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO9053099.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPPQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            12481..13767
                     /gene="dctA"
                     /locus_tag="ACHKFK_002806"
     CDS             12481..13767
                     /gene="dctA"
                     /locus_tag="ACHKFK_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO9053100.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            13987..15483
                     /locus_tag="ACHKFK_002807"
     CDS             13987..15483
                     /locus_tag="ACHKFK_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9053101.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPVQ"
     gene            complement(15579..16508)
                     /gene="kdgK"
                     /locus_tag="ACHKFK_002808"
     CDS             complement(15579..16508)
                     /gene="kdgK"
                     /locus_tag="ACHKFK_002808"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO9053102.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWRGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAEDAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            16739..17506
                     /gene="pdeH"
                     /locus_tag="ACHKFK_002809"
     CDS             16739..17506
                     /gene="pdeH"
                     /locus_tag="ACHKFK_002809"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO9053103.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVMHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIEDLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            17576..19636
                     /locus_tag="ACHKFK_002810"
     CDS             17576..19636
                     /locus_tag="ACHKFK_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO9053104.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FQLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLIYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGIAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLDIANGVARPQIFAFDTENALINITGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMRK"
     gene            complement(19677..20999)
                     /locus_tag="ACHKFK_002811"
     CDS             complement(19677..20999)
                     /locus_tag="ACHKFK_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053105.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFAVMVPLAGLLADAFGRRKSMVVITTLIILFALF
                     AFNPLLGSGNPALVFVFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(21392..22405)
                     /gene="yhjD"
                     /locus_tag="ACHKFK_002812"
     CDS             complement(21392..22405)
                     /gene="yhjD"
                     /locus_tag="ACHKFK_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO9053106.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(22454..23353)
                     /locus_tag="ACHKFK_002813"
     CDS             complement(22454..23353)
                     /locus_tag="ACHKFK_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9053107.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARSLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            23873..24475
                     /locus_tag="ACHKFK_002814"
     CDS             23873..24475
                     /locus_tag="ACHKFK_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFO9053108.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQHRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTAVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(24526..26175)
                     /locus_tag="ACHKFK_002815"
     CDS             complement(24526..26175)
                     /locus_tag="ACHKFK_002815"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO9053109.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARNWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            26580..27977
                     /gene="ccp"
                     /locus_tag="ACHKFK_002816"
     CDS             26580..27977
                     /gene="ccp"
                     /locus_tag="ACHKFK_002816"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO9053110.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LANKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDRWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT         6624 a         7078 c         7738 g         6563 t
ORIGIN      
        1 ccgccgcatt agcctggtca ttacaaatgt tgggagaaaa tgtcctggtg gtcgatgcct
       61 gcccggacaa cttgttgcgc ctgtcattta acgttgattt tacccaccgt cagggttggg
      121 ccagagctat gctggatggc caggactggc gtgacgccgg gttgcgctat acctcgtagc
      181 tcgatttgct gccttttggt cagttatcca ttgaagaaca agaaaatcca cagcactggc
      241 aaacccggct gagcgatatt tgcaccggct tacagcaact aaaagccagc gggcgttacc
      301 agtggatttt aattgactta ccgcgtgatg cctcgcagat aacccaccag ctgctgagtt
      361 tgtgcgatca ctcgctggca atcgtcaatg tggatgccaa ctgccatatc cgactgcatc
      421 agcaagcgct gccggatggt gcacatattt tgattaatga cttccgtatt ggcagccagg
      481 ttcaggacga tatttaccag ctttggttgc aaagccagcg ccgattgctg ccgatgctca
      541 ttcatcgtga tgaagcgatg gctgaatgtc tggcagctaa gcaaccagtc ggtgaatatc
      601 gcagtgatgc gctggcggct gaagagatat tgacgctggc gaactggtgc ctgttgaatt
      661 attccgggct gaaaacgcca gtcgggagtg catcatgagt atcctgaccc ggtggttgct
      721 tatcccgccg gtcaacgcac ggcttattgg gcgttatcgc gattatcgtc gtcacggtgc
      781 gtcggctttc agcgcgacgc tcggctgttt ctggatgatc ctggcctgga tttttattcc
      841 gctggagcac ccgcgctggc agcgtattcg cgcagaacat aaaaacctgt atccgcatat
      901 caacgcctcg cgtccgcgtc cgctggaccc ggtccgttat cttattcaaa catgctggtt
      961 actgatcggt gcatcgcgca aagaaacgcc gaaaccgcgc aggcgggcat tttcaggtct
     1021 acaaaatatt cgtggacgtt accatcaatg gatgaacgag ctgcctgagc gcgttagcca
     1081 taaaacacag catcttgatg agaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg
     1141 gttgatcctt ggtatcatcg tcaccttctc gctgattctg gcgttaatct gcgttactca
     1201 gccgtttaac ccgctagcgc agtttatctt cctgatgctg ctgtgggggg tagcgctgat
     1261 cgtacggcgg atgccggggc gcttctcggc gctgatgctg attgtgctgt cgctgaccgt
     1321 ttcttgccgt tatatctggt ggcgttacac ctcgacgtta aactgggacg atccggtcag
     1381 cctggtgtgc gggcttattc tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct
     1441 cggctatttc caggtcgtat ggcctctgaa tcgtcagccg gtgccattgc cgaaagatat
     1501 gtcgctgtgg ccgtcggtgg atatctttgt cccgacttac aacgaagatc tcaacgtggt
     1561 gaaaaatacc atttacgctt cgcttggtat cgactggccg aaagacaagc tgaacatctg
     1621 gatcctagat gacggcggca gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa
     1681 atatatcgcc cgtaccactc atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa
     1741 atatgccaaa ggcgagttcg tgtcgatatt cgactgcgac cacgtaccaa cgcgatcgtt
     1801 cctgcaaatg accatgggct ggttcctgaa agagaaacag ctggcgatga tgcagacgcc
     1861 gcaccacttc ttctcgccgg acccgtttga acgcaacctg gggcgttttc gtaaaacgcc
     1921 gaacgaaggc acgctgttct atggtctggt gcaggacggc aatgatatgt gggatgccac
     1981 tttcttctgc ggctcatgtg cggtgattcg ccgtaagccg ctggatgaga ttggcggtat
     2041 tgctgtcgaa acggtaaccg aagatgcgca cacttctctg cgtctgcacc gtcgtggcta
     2101 cacctccgcg tatatgcgta ttccgcaggc agcggggctg gcgaccgaaa gtctctcggc
     2161 gcatatcggt cagcgtattc gctgggcgcg cgggatggtg caaatcttcc gtctcgataa
     2221 cccgctcacc ggtaaagggc tgaagtttgc tcagcggctg tgctatgtca acgccatgtt
     2281 ccacttcttg tcgggcattc cacggctgat cttcctgact gcgccgctgg cgttcctgct
     2341 gtttcatgcc tacatcatct acgcgccagc gttgatgatc gccctgtttg tgctaccgca
     2401 tatgatccat gccagcctga ctaactcgaa gatccagggc aaatatcgcc actctttctg
     2461 gagtgaaatc tacgaaacgg tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct
     2521 gattaacccg cacaaaggca aatttaacgt caccgccaaa ggtggactgg tggaagaaga
     2581 gtacgtcgac tgggtgatct cgcggcccta catcttcctt gttctgctca acctggtggg
     2641 cgtagcggtt gggatctggc gctacttcta tggtccgcca actgagatgc tcaccgtggt
     2701 cgtcagtatg gtgtgggtat tctacaacct gattattctc ggcggcgcag tcgcagtatc
     2761 ggtagaaagc aaacaggtac gccgatcgca ccgcgtggag atgacgatgc ccgcagcaat
     2821 tgcccgcgaa gatggtcacc tcttctcgtg taccgttcag gatttctccg acggtggttt
     2881 ggggatcaag atcaacggtc aggcgcagat tctggaaggg cagaaagtga atctgttgct
     2941 taaacgcggt cagcaggaat acgtcttccc ggctcaggtg gcgcgcgtga tgggtaatga
     3001 agttgggctg aaattaatgc cgctcactac ccagcaacat atcgattttg tgcagtgtac
     3061 gtttgcccgt gcggatacat gggcgctctg gcaggacagc tacccggaag ataagccgct
     3121 ggaaagtctg ctggatattc tgaagctcgg cttccgtggc taccgccatc tggcggaatt
     3181 tgcgccttct tcggtgaagg gcatattccg tgtgctgact tctctggttt cctgggttgt
     3241 atcgtttatt ccgcgccgcc cggagcggag cgaaacggca caaccatcgg atcaggcttt
     3301 ggctcaacaa tgatgataac gcgatgaaaa gaaaaatatt ctggatttgt gcagtggctc
     3361 tggggatgag tgcgttcccc tctttcatga cgcaggcgac gccagcaacg caaccactga
     3421 tcaatgctga gccagctgta gccgcccagg cggagcaaaa tccgcaggtg gggcaagtga
     3481 tgccgggcgt gcagggcgca gatgcgccag tcgtggcgca gaacggtcct tcccgtgatg
     3541 tgaagctgac ctttgcgcaa attgcgccgc cgccgggcag catggtgcta cgtggcatta
     3601 acccaaacgg cagcattgag tttggtatgc gcagcgatga agtggtgacg aaggcgatgc
     3661 tcaacctcga atacacgcca tcgccatcat tactgcctgt ccagtcgcag ttaaaggttt
     3721 atctcaatga tgaactgatg ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa
     3781 cgctggcaca aatgcccatt aacccgctgt ttattactga tttcaaccgt gtgcggctgg
     3841 agtttgtcgg ccattatcag gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg
     3901 atgttgggcg gagcagtgga ctggatctga cctatcagac cctgaatgtg aagaatgacc
     3961 tgtcacactt cccggtgcca ttctttgacc cgcgtgataa ccgcaccaac accttgccga
     4021 tggtctttgc gggtgcgccg gatgttgagc tgcaacaagc atctgccatt gtcgcctcgt
     4081 ggtttggttc gcgttctggc tggcgtgggc agaacttccc ggtgctctat aaccaactgc
     4141 cggatcgcaa tgccattgtc tttgccacca atgacaaacg cccggacttc ctgcgcgatc
     4201 atccggcggt aaaagccccg gtgattgaga tgattaacca tccgcagaat ccttacgtca
     4261 aactgctggt ggtgtttggt cgtgacgaca aagacttgtt gcaggcagcg aaaggtatcg
     4321 ctcagggtaa cattctgttc cgtggtgaaa gtgtggtagt gaatgaagtg aaaccgctgc
     4381 taccacgtaa gccgtacgat gcgccgaact gggtgcgtac cgatcgtccg gtcacctttg
     4441 gtgaactgaa aacctatgaa gaacaattac aatccagcgg tcttgagcca gcagcgatta
     4501 acgtttcgct aaacctgccg ccggatctct acctgatgcg cagtaccggc attgatatgg
     4561 atattaatta ccgctacacc atgccgccgg tgaaagacag ttcgcggatg gatatcagcc
     4621 tgaataacca gttcctgcaa tccttcaacc tgagcagcaa acaggaggcg aaccgcctgc
     4681 tgctgcggat tccggtatta caaggtttgc tggatggcaa aacggatgtc tctattccgg
     4741 cgctgaaact gggcgcgacc aaccagctgc ggttcgactt tgagtatatg aacccgatgc
     4801 cgggcggttc ggtggataac tgcattacct tccagccggt gcagaatcat gtggtgattg
     4861 gtgacgactc caccatcgac ttctcgaagt attaccactt catcccgatg ccggatctac
     4921 gcgcctttgc taacgcgggc ttcccgttca gccggatggc ggatttgtcg caaaccatca
     4981 ccgtgatgcc gaaaacgcca aacgaagcac agatggaaac cttgctgaat accgttggct
     5041 ttatcggtgc gcagacgggc ttcccggcga ttaacctgac ggtgaccgat gatggcagca
     5101 ccattcaggg caaagatgcc gacatcatga tcgtcggtgg tatcccggac aaactgaaag
     5161 acgataagca gatcgacctg ttggtgcagg ctaccgaaag ctgggtgaaa acgccgatgc
     5221 gccagacccc gttccccggt attgtgccgg acgagagcga tcgcgcggca gaaacccagt
     5281 caacgctgac ctcttccggt gcgatggcgg cggtgattgg tttccagtcg ccgtataacg
     5341 accagcgcag cgtgattgcg ctgctggcag atagcccacg cggttatgaa atgcttaacg
     5401 atgcggtgaa cgatagcggc aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg
     5461 agtccggtat caacagccta cgtgttggcg atgtttatta cgtaggtcat ctgccgtggt
     5521 tcgagcgctt gtggtatgcg ctggcaaacc atccgattct gctggcggtg ctggcggcta
     5581 tcagtgtgat attgctggcg tgggtactgt ggcgtctgct gcgtattatt agtcgtcgtc
     5641 ttaacccgga taacgagtaa ttgaagatga atgtgttgcg tagtggactc gtgacgatgc
     5701 tgctgctggc tgcctttagt gttcaggcag cctgtaccta gcctgcctgg gagcagttta
     5761 aaaaggatta catcagtcag gaagggcgcg tcatcgaccc cagcgacgcg cgcaaaatca
     5821 ccacctccga agggcaaagt tacggcatgt tctttgccct ggcggctaac gaccgtgcag
     5881 ctttcgataa tcttctcgac tggacgcaga acaatctcgc tcagggttct ttaaaagaac
     5941 atttgcccgc ctggctgtgg ggcaagaaag agaacagtaa gtgggaagtg ctggacagca
     6001 attcggcctc cgatggtgat gtctggatgg cctggtcgtt gctggaggcg gggcgtttgt
     6061 ggaaagagca gcgttatacc gacatcggca gcgcattgct aaaacgtatc gcgcgggagg
     6121 aagtggtgac ggtgcctggg ctgggctcca tgttgttacc gggcaaagtg ggttttgctg
     6181 aggataacag ctggcgtttt aaccccagct acctgccgcc gacgctggcg cagtatttca
     6241 cccgctttgg cgcgccgtgg accacgctgc gcgaaaccaa tcaacgttta ttgctggaaa
     6301 ccgccccgaa aggcttttcg ccagactggg tgcgctatga gaaagacaaa ggctggcagc
     6361 taaaagccga aaaaacattg atcagcagct acgacgctat ccgcgtttac atgtgggtag
     6421 gcatgatgcc tgacagcgat ccgcaaaaag cgcggatgct caaccggttt aaaccgatgg
     6481 cgacattcac tgagaaaaac ggttatccgc cggaaaaagt ggatgtggct acggggaaag
     6541 cgcagggtaa aggaccggtc ggtttttctg ccgccatgct gcccttttta caaaaccgcg
     6601 atgcgcaggc cgttcagcgc cagcgcgtgg ccgataactt tcccggcagc gatgcctatt
     6661 acaactatgt gctgaccctg tttggacaag gctgggatca acaccgtttc cgcttctcga
     6721 caaaaggtga gttattacct gactggggcc aggaatgcgc aaattcacac taaacatatt
     6781 cacgctttcc ctcggtctgg ccgtcatgcc gatggtcgag gcagcaccaa ccgctcagca
     6841 acagttgctg gagcaagttc ggttaggcga agcgacccat cgtgaagacc tggtgcaaca
     6901 gtcgttgtat cggctggaac ttattgatcc gaataacccg gatgtgattg ctgcccgttt
     6961 tcgttctctg ttacgtcagg gcgatattga tggcgcacaa aaacagctcg atcggctgtc
     7021 gcagttagcg ccgagttcaa atgcgtataa atcgtcgcgg accacaatgt tactgtctac
     7081 gccggatggt cgtcaggcac tgcaacaggc tcgattgcag gcgactaccg gtcatgcgga
     7141 agaggccgtg gcgagttaca acaagctgtt caacggtgcg ccgccggaag gtgacattgc
     7201 tgtcgagtac tggagtacgg tggcgaaaat tccggctcgc cgtggtgaag cgattaatca
     7261 gctaaaacgc attaatgcgg atacgccggg caatacgggc ctgcaaaaca atctggcgct
     7321 attactgttt agtagcgatc gccgtgacga aggttttgcc gtcctggaac agatggcgaa
     7381 atcgaacgcc gggcgcgaag gggcctctaa aatctggtac gggcagatta aagacatgcc
     7441 cgtcagcgat gccagcgttc aggcactgaa aaaatatctc tcgatcttta gcgatggcga
     7501 tagcgtggct gctgcacaat cgcaactggc agaacagcaa aaacagctgg ccgaccctgc
     7561 tttccgcgct cgtgcgcaag gtttagcggc ggtggactct ggcatggcgg gtaaagccat
     7621 tcccgaacta caacaggcgg tgcgggcgaa cccgaaagac agtgaggctc tgggggcgct
     7681 gggccaggcg tattcccaga aaggcgatcg cgccaatgca gtggtgaatc tggaaaaagc
     7741 cctcgcgctg gatccgcaca acagcaataa cgacaaatgg aacagcctgc tgaaagtaaa
     7801 ccgctactgg ctggcgatcc agcagggcga tgccgcgctg aaagccaata atcctgaccg
     7861 ggcagaacgg ctgttccagc aggcacgtaa tgtcgataac accgacagct atgcagtgct
     7921 tgggctgggc gatgtggcga tggcgcgcaa agattatccc gccgccgaac gctattacca
     7981 gcagaccttg cgtatggaca gcggcaacac taacgccgtg cgcgggctgg cgaatattta
     8041 ccgccagcag tcgccagaaa aagccgaagc gtatatcgcc tcgctctctg ccagccagcg
     8101 gcgcagcatt gatgatatcg aacgcagcct gcaaaacgac cgtctggcac agcaggcaga
     8161 ggcactggaa aaccagggga aatgggcgca ggcggcagca cttcagcggc aacgactggc
     8221 gctggacccc ggcagtgtat ggattactta ccgactttcg caggatctct ggcaagccgg
     8281 acaacgcagc caggccgata cgttaatgcg caatctggcg cagcagaagc cgaacgaccc
     8341 ggagcaggtt tacgcttacg ggctgtacct ctccggtcac gaccaggaca gagcggcgct
     8401 ggcgcatatc aacagcctgc cgcgcgggca gtggaacagc aatattcagg agctggttaa
     8461 tcgactgcaa aacgatcagg tgctggaaac cgctaatcgc ctgcgagaaa acggtaaaga
     8521 ggcagaagcg gaagcgatgc tgcgccagca accgccttcc tcgcgtattg acctcacgct
     8581 ggctgactgg gcgcagcagc gacgtgatta caccgccgcc cgcgctgcat atcagaatgt
     8641 cctgacgcgg gagccaacta acgccgatgc cattcttggt ctgacggaag tggatattgc
     8701 tgccggtgac aaagcggcgg cacgtagcca gctggcgaaa ctgcctgcca ttgataacgc
     8761 ctcgctgaac acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc
     8821 ggcgcagcag acatttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga
     8881 aagcgcgatg gtactgcgtg atggtgcgaa gtttgaagcg ctggcgggcg atccaacgca
     8941 ggcgctggaa acctacaaag acgctatggt tgcatccggt gtgaccacga cgcgtccgca
     9001 ggataacgac acctttaccc ggctgacccg taacgacgag aaagatgact ggctgaaacg
     9061 cggcgtgcgc agcgatacgg cggacctcta tcgccagcag gatcttaacg tcactctcga
     9121 gcacgattac tggggttcga gcggtaccgg tggttactcc gatctgaaag cgcacaccac
     9181 tatgttgcag gtggacgcgc cctattctga cgggcggatg ttctttcgca gtgatttcgt
     9241 caatatgaac gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg
     9301 cacctgtaca ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt
     9361 ggcggtcggc tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt
     9421 caacgtggtg gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta
     9481 caccgttaac gcccaccgtc ggcctatctc cagttctttg ctggcctttg gtgggcaaaa
     9541 agactctccg agcaataccg ggaaaaaatg gggcggcgtg cgtgccgacg gcgtggggct
     9601 aagtctgagc tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gcggtgatca
     9661 gttaaccggt aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta
     9721 taaggtcatc aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca
     9781 ttatgacaaa gacctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga
     9841 atacctgtcg tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga
     9901 gctgggggcg tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct
     9961 gatgaatctg atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg
    10021 cagcagtcag ggctttggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa
    10081 ctggtttgtc ggcacggcaa ttgatatcca gcaggcaaaa gattacgccc ccagccattt
    10141 cctgctctac gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca
    10201 gccgctgata ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg
    10261 cgatcgggta tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag
    10321 tcgctcgtta acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt
    10381 tattttttgc accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac
    10441 gcaactggaa agcattgctc gctctgtccg cgaaccctta tcttctgcca ttttgaaagg
    10501 cgatattccc gaagcggaag ctattcttgc cagcattaaa ccggccggcg tggtcagccg
    10561 tgccgatgta gtgctgccca accagttcca ggcgctgcgt aaaagtttta ttccagagcg
    10621 tccggtgccg gtaatggtta ctcgcctgtt tgagctaccg gtgcaaatct cgctgggcgt
    10681 ttactcgctg gaacgtccgg caaacccgca gccaattgcc tatctggtgt tacaggcgga
    10741 ttccttccgc atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt
    10801 acttttgtcg ttaatattga cggtggcgat tagctggtgc attaaccgcc tgattttgca
    10861 tccgttacgc aatattgctc gcgaacttaa cgccatccca ccccaggagc ttgttggtca
    10921 ccaactggca ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta
    10981 caacctcaac cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg
    11041 cttcccggtg tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggtggt
    11101 ggcgcgtaaa caaactaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc
    11161 gggtgtgctg aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa
    11221 atcggtactg tcgccacgca tgatcctcgc gcagattagc ggttatgact ttgctgtcat
    11281 tgccaacggt gtacaggaac cgtggcacgc tatcacatta ggtcagcaag tgctcactat
    11341 catgagcgag cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt
    11401 ggcgatgttc tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc
    11461 atttaccgct cgccataaag gcaaaaatca gattcagttc tttgatccgc agcagatgga
    11521 agccgcccag aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca
    11581 gtttgcgatt tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga
    11641 agtgttactg cgtatccagc aaccggatgg cagttgggac ctgcccgatg gcttaatcga
    11701 tcgcattgag tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg
    11761 tcgattgctt gctgcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc
    11821 agcgctgcaa ctgatgcatc caaatatggt ggcggatatg ctggaactgt taacccgcta
    11881 tcgcattcag ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgatcc
    11941 tcatgctgcg gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga
    12001 tgatttcggc atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat
    12061 cgacgtactg aaaatcgaca aaatgtttgt tgaaggtttg ccggaagata gcagcatgat
    12121 tgctgcaatt atcatgctgg cgcagagcct gaacctacaa atgattgccg aaggcgtgga
    12181 gactgaagca caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct
    12241 ttttgctcgc ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct
    12301 accccaaact gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt
    12361 ttaacctttt tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat
    12421 tttaaggccg caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct
    12481 atgaaaacct ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt
    12541 attctccttg gccatttcta ccctgaaata ggcgagcaaa tgaaaccgct tggcgacggc
    12601 ttcgttaagc tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc
    12661 attgcgggca tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac
    12721 tttgaaattg tcagcaccat cgcactgatt attggtctta tcatcgttaa cgttgtgcag
    12781 cctggtgccg gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac
    12841 gccgatcagg cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc
    12901 gtcattggcg catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt
    12961 ggttttgcgc tccaccgtct gggcagcaaa ggccaactga tttttaacgt gatcgaaagt
    13021 ttctcgcagg tcattttcgg catcatcaat atgatcatgc gtctggcacc tattggtgca
    13081 ttcggggcaa tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg
    13141 cagctgatta tctgtttcta catcacctgt atcctgtttg tggtgctggt attgggttca
    13201 atcgctaaag cgacgggttt cagtatcttc aaatttatcc gttacatccg tgaagaactg
    13261 ctgattgtac tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg
    13321 gagaaactcg gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt
    13381 aaccttgatg gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact
    13441 aacagtcaga tggatatcgt ccaccaaatt acgctattaa ttgtgttgtt gctctcttcc
    13501 aaaggggctg ccggggtaac gggcagtggc tttatcgtgc tggcggcgac gctctctgcg
    13561 gtgggccatt tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca
    13621 gaagctcgtg cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag
    13681 tgggtgaaag aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat
    13741 ggcaaaacgc acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga
    13801 gtgcgggtgt ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag
    13861 gtttatttca cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt
    13921 aacgtcattt agactgaccg acaacgggca gttttttatt accaggatag ttgatcaggg
    13981 gttcacatgc agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact
    14041 gctggctatg tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt
    14101 tccaacggtt tacagtggca agtgctgact acgccccaac gtcccagcga tcgtgttgaa
    14161 attcgcctgc tggttaatac cggttcgctc gccgaaagta cacaacagag cggttacagt
    14221 cacgccatcc ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc gcaggcgcgt
    14281 tcattgtggc agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat
    14341 gacaccacgc tgtttaatct gagtttgccc aataaccgta acgacttgct gaaagaggcg
    14401 ctctcttatc tggcaaatgc cactggcaaa ctgactatca cgccagaaac catcaaccat
    14461 gcgttgcaaa gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg
    14521 cgttatcgtc tgaaaggatc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa
    14581 cccgttgaag cggaaaagat taaagatttc tatcagaaat ggtacacccc ggatgcaatg
    14641 acgctgctgg tggtgggaaa cgtggatgcg cgctcggtcg tcgaccaaat caataaaacg
    14701 tttggcgaac tgaaaggcaa acgtgaaaca ccggctccgg tgccgacgct ttctccgctg
    14761 cgtgcggaag cggtgagtat tatgaccgac gcggtgcgtc aggaccggct atctatcatg
    14821 tgggatacgc cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg
    14881 gacctggccc gtgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagt
    14941 aaagacatcg gtcttggatt tgactgtcgt gtgctgtatc tgcgtgcgca gtgtgccatc
    15001 aacatcgaat caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg
    15061 gcgaaggttc gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag
    15121 aaactggagc tgcagaaact gtttgccgcc tatgcgcgag ctgataccga tattctgatg
    15181 gggcagcgga tgcgttcgtt gcaaaatcag gtcgtcgata tcgcgccgga gcaatatcag
    15241 aaactgcgtc aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt
    15301 cagcagttgt cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt
    15361 aacatgaaag cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgcggctgcc
    15421 gccacatctg tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgtacag
    15481 taatgcctga tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga
    15541 ggggtaacac tctatccagc cttttgcata tgctgctttt acgctggcat cgcttcacgc
    15601 gggataatcg cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgct
    15661 gcgtcttccg cactgccgcc cgtcaaacgt accgccagat aaccggcact gaaagagtcg
    15721 ccagctgcgg tggtatcgat cactttttct ttcggcagtt ttaccgccgg aacatccact
    15781 aacccttcgc cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct
    15841 ttcacgcccg cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc
    15901 gcgtcttcat cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac
    15961 acctgctgtg tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc
    16021 acttttccgc cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc
    16081 gggcttaaga tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct
    16141 tcgcaaatcg ccgcggattg ctcactctcc agccagaatt tggcggcggc ttcgttacgc
    16201 cagtagtaga acgtacgttc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga
    16261 cggttttcca tccgttgggt cagggaagta tcaacgttct cgccgcgcca ggcgtccagc
    16321 atctgctggc taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca
    16381 ggatcgacct gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc
    16441 ttaacgtccg cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt
    16501 ttggacatag tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt
    16561 tgctggtcaa ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg
    16621 aaagattccg ttgtggagtg gggaaattta ccccgccagg cggtaaagtt ctgcccttgc
    16681 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    16741 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    16801 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccaatct atcaaacatg
    16861 cgggcggtta atggccgtgg agctattaac ggtggtcatg catcccttga acccttcgca
    16921 acgcctgccg ccggatcgct attttactga aatcaccgtt agccatcgga tggaggttgt
    16981 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttgtagagc acggcctgct
    17041 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    17101 gcgccagatt gaggatcttc cctggctgcg tttcgaactg gtggaacaca tccgcctgcc
    17161 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg atgattttgg
    17221 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    17281 gcgagaactg tttgtgatgc tgcgtcagtc accggaagga cgcacactct tttctcagct
    17341 tttacatcta atgaatcgct attgtcgcgg ggtgattgtc gagggcgtag aaacaccgga
    17401 agagtggcgt gatgttcaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    17461 cccggcaccg atagaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    17521 cgcctggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    17581 caaggcaggc aaaataaccg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    17641 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    17701 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg
    17761 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg ccccatgtac acgcggaaga
    17821 catcattctt ggcaatccac cggatattcc cgaagtcaca atggtgcatt tgccgcgagt
    17881 agaagcgacg ctggccccgc tggcgctgct gaccaaaacg gtctggttgc cgtggatcaa
    17941 gctcgaaaag cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt
    18001 taatctcgcc aacgatgaca acaaagacgc gaatgcaaag ccgtcggcgt ggtcgtttca
    18061 gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc
    18121 ggatctggaa atttttgtcg atccgttagg caagccgctg ccgttcagcg aagttaccgg
    18181 atcgaaaggt aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca
    18241 gggacgttat aacggtgaac cgctcactgg tacgggaaaa ataggcggta tgctggcgct
    18301 gcgtggcgaa ggaacgccgt ttccggtaca ggctgatttc cgctctggta acacccgtgt
    18361 tgcttttgat ggcgttgtga atgacccaat gaagatgggg ggtgtcgatt tacggcttaa
    18421 attttctggc gattcgctgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac
    18481 accgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt
    18541 ctttgattat cgcggtttta atgggcgcat tggcgatagc gatatccacg gttctctgat
    18601 ctacaccacc ggcaagccac gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg
    18661 gctggcggac ctgggaccgt tgattggcgt tgattccggg aaaggggcag aaaagtcgaa
    18721 acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta
    18781 tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaagggcg
    18841 gcgcattgag catggtagca gcctgccgat tagcgatctt tctactcata tcatcctcaa
    18901 aaatgctgac ctgcgtctgc aaccgctgaa atttggcata gcgggcggca gcattgcggc
    18961 gaatattcat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg
    19021 tcgactgaaa ctgaaagaac tgatgcccga tgtagaactg atgcagaaga cgctggggga
    19081 aatgaacggt gacgcggaac tgcgcggtag cggtaactcg gtggcggcgc ttttaggcaa
    19141 cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga
    19201 gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg atgatgaggt
    19261 gcgggtgaac tgcgcggcgg cgaatctgga tattgccaac ggcgtggcgc gcccgcagat
    19321 ttttgctttc gatactgaga acgcgttgat taacattacc ggcacggcaa gttttgcttc
    19381 ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg
    19441 ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccgggcc
    19501 gttgattgct cgcggagccg ttgcggcggc actggcaacg ctggtaacac cggcggcggc
    19561 gttactggcg ctgatctcac cttccgaagg ggaggctaat cagtgcagga caattttgtc
    19621 gcagatgaga aagtgatgat aattgccgga tgtctgacat ccggcagtta aaacgactac
    19681 aacgactggt gccgcgtctc atgcgttaac agcagggcaa ttaacgtcag tccggccatc
    19741 gccgccagat ataaccccac cgcgcctaac ccatagttag tctgcaacca ggctgcgata
    19801 tacggcgcaa cagacgcccc gagaatcgac gatacgttat aggagaacga tgctccggta
    19861 taacggactt ccgtcgggaa cagctccggc agcagcgccc ccatcggacc aaaggtcagg
    19921 cccatcaaac tcaagcccag cagcaggaaa acaaacacca atgctggatt accagaaccc
    19981 aacagtgggt taaaggcaaa cagggcaaac agaatgataa gcgtagtgat caccaccata
    20041 cttttacggc gaccaaaagc atctgccagc agacccgcca acggaaccat cactgcaaaa
    20101 ccgataaccg ccatcatcaa catccacagt acttcgttac gcggtaaacc aagcccaact
    20161 ggcgcggcgg cggtactaaa ggtcatagag tagaccgtca tgatgtaaaa cagcgtatag
    20221 gttgccagca taatgaacgt acccagtacg gttacgcgaa catgtttggt cagcagcgta
    20281 cccagcggga tcttcacctg ttttttcgct ttagcgactt tctcaaacac cggcgactca
    20341 tgcagcgaca cgcgaacata caggccgata atgaccagca ccgccgagaa gataaatggc
    20401 acacgccagc cccagctcat aaactgctcg tcggtcagta gccaggaaag cagcaagaaa
    20461 gtgccgttgg caaagaagaa gccaatcggt gcgcccagct gcgggaagga gccgtacaat
    20521 gcacgtttgc gcggtggggc gttttcagtc gccagcagcg ccgcgccgcc ccattcacca
    20581 cctaagccca gaccctgacc aaatcgagcc agcgccagca gcagcggggc gaaaatacca
    20641 atcgtggcat agcctggcag cagaccaatc accacggtcg aaatccccat cgttagcaac
    20701 gaggcgacca gcgtcgcttt acgcccaacg cgatcgccaa aatgaccaaa aacggcagag
    20761 ccaatggggc gcgcgacgaa ggcgatggca aaggtggcga gcgactgtag cgttgctgcc
    20821 gttggatcgc cctgcgggaa gaagatatgc ggaaacacaa taacggccgc agtggcgtaa
    20881 atgtaaaagt cgaagaactc aatggccgtg ccaatgagag aagcgacaag gactttatta
    20941 cgcgagttga tcggcgtgta ttcttgctcg tggtcgagtg ttgtggctgt tgcttgcata
    21001 atcttttctt attttttggc taacgaatag ccatattacg cacagcaaaa gtgatatttc
    21061 aatgtgtaac aaatcgcgga aatggtcaaa aaacaggcaa aaaggggata atgtttaggc
    21121 cagtgatttt tcttctatga aatgcttcac ataattttat aaatggtgga ttaaactggt
    21181 tttaggttaa ataaaagtta ccaactggat ttatatgctg gggttatgag ttggaataaa
    21241 attgtttgct cctgaataga tggctaaaga ggctctggga aagagtttca agaggttatg
    21301 tgcgcattat agccggatgc gatgctggcg catcttatcc ggcctacggg gagccaggcc
    21361 gaataaattc gtacgccgca tccggcctat tttaaggctg cgttttcccc ggcattcgcg
    21421 ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca aaacagcgtc aaacgggcga
    21481 agaagtagaa aaatgccatc agccccagca cggaaccaaa tgctgcgcca gagggtgatt
    21541 tcatcaacga tggcagggtg taggtcatca cgattttaat cacttcaaaa ccaatagcag
    21601 ccagaaatgt tccgcggatc agggcttttt tgcgcggacg gtggcgcggc aaccgccaga
    21661 agatccagaa gaaaagcaga tagttggcga agatggaaat cgccaggcca atcaaccgcc
    21721 atgtcggttt cagccactca atgctgttca ggtgcagggc gctaataatc atttgctgcg
    21781 cagaaccggc aaccgaggtg atagaaagcg tcacaatcag cgcaatcaac aaaccaatca
    21841 gcgaaataaa atcacgcaga tattttaccc agaacttctc ctgatcttgc ggcgagcgtt
    21901 cccagacatc gcgtgactgg gcgcgaatcg cttcacgcag gttacccatc cagttgatgc
    21961 cggaataaag cgccactgcc aggccgacaa gccctacagt cgtacgctgc tgaacggcgg
    22021 tgttgatggt gtttttcaac gtggcggcta gcgtcggatc gctaatgttt tgcagaattt
    22081 tgtcgaagat atcctgtagc aacatcggat gggaggccag cacaaagccc cctgcggcaa
    22141 acgacaccat caaaatgggg atcatcgaca aaaatgagaa ataggtgata gccgccccaa
    22201 actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg aatcaggtgc gcaataaccg
    22261 gttggcgttg gactttctcg gcagtggtgg tgacggtttc cagcgcctgg ctaactttgc
    22321 cgcctttctc gctattatcc aactgcttaa tcggctcgtg ctccagatcc tgggtgggac
    22381 gtttgatctc gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg tcattcttaa
    22441 aaagtatagt cggtcagtcc aggtgctctt tcattacgcc agccagccac tgcataaaca
    22501 ggtttacacg ccgggaaagc tcccgacgct gcggataaac cagtgaaagg gacagcggct
    22561 cggcacggta gccaggcaac acttcaataa gccgcccggc acgcagggct tcgcgcacgg
    22621 caatgcgcgg gatctgaata atccccagac cggcaagacc ggcggcgaga tagttttcgc
    22681 tactgtttac cgtcaacatg ccgccagact taaaccactg gacgccatta acgctggcaa
    22741 cctcaaaacc taacggatgt acacccaggt gcggtgtata acgcactatt gcgtgtgaag
    22801 tcagatcatc ggggctttgc ggataaccaa agcgcgtcag atagtgtgga ctggcacagt
    22861 tgaccatggt cagtttgccg agagaacggg cgataacgcc atcttccggt aatgcgccag
    22921 tgcgtatcac gcaatcaaaa ccatcatgaa gaatatctac cggacggtca tggctactca
    22981 gttccagctc aattcccgga tgcagataga gaaattccga caagcgcggc agtaacaggc
    23041 ttttcgcgat tcctgacggg atgtcgatgc gtaatttacc gctgatactg gtggcatcct
    23101 gttgaaacag accgtccagt tcgctgagat tactcaacac atcttttgct cgttgataat
    23161 aggtcatgcc ttctggcgtg agtttgaccc gtcgcgtggt gcgctggaga agctgggtgc
    23221 caagttgatg ttccagtgcc tgtatctggc gcgaaacact tccctttggc aaagcaaaga
    23281 aatccgccgc gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac tgcattgcgt
    23341 gaattttatc catttctttg accgattgtt gttcacaacg aaacagtagc gcgcatatcg
    23401 cattgtttat tgttttttct aacatctaat gccgggtttc acaatgttgt catgatgaat
    23461 gtggcgattt ctcagcagga aaagggagga atagctttat gagaaaggtg aattccctta
    23521 aacagagcgt atttttcagc gattcccttt aaagtgaata gaagatgtat tggctatttg
    23581 tgcttatttt tatctcattg ccgtttgtta tttttgttta tggtcgcgga atttcatttt
    23641 taattgcatg cgtactaact accgatataa ggaaatcctc aaaaatgttt taaatagtgc
    23701 cctcgacatg taatttcagg cgtttagctg taactattta tatcggtaac tattttctac
    23761 gcgttatctc tgtagaatgg ccaccgccaa atggcgaatt cttatttttt ctcgcgggta
    23821 atacccttcc actacttaag tctttttaac gtattcaggt ggagattcag ttatgcaaat
    23881 agtcatgttt gacaggcagt caatatttat tcatggaatg aaaatcagtt tacagcatcg
    23941 tattccagga gtgagtattc agggggccag tcaggcagac gagttatggc aaaagctgga
    24001 aagttaccct gaagccttag tgatgctcga tggcgatcag gatggtgagt tttgctactg
    24061 gttgctgcaa aaaaccgcgg tgcaatttcc tgaggttaag gtgttaatta cggcgacgga
    24121 ttgcaacaaa cggtggttac aggaagttat tcattttaat gtgctggcca ttgtgcctcg
    24181 tgattcaacc gtcgagacat ttgcgttggc ggtcaatagt gcggcgatgg ggatgatgtt
    24241 tctgcctgga gactggcgaa cgacgccgga aaaggacatt aaagatctca aatcgttaag
    24301 cgcccgccag cgggagattt taaccatgtt agcggcgggc gaatcaaata aagagatcgg
    24361 cagggcgctg aatatcagta ccggaacggt aaaagcgcat ctggaatcac tgtatcgccg
    24421 tctggaagtc aaaaatcgca cccaggcggc aatgatgtta aatatctcct cctgaagagg
    24481 ataattttac agccagcagg aagttagcgg ctggctgtaa aaatattatg gttcgccgta
    24541 caaaccaatt aaacggcgta ccacaccgtt agtccagcca aacccatcct gcaacggata
    24601 ctcgccaccg ccgccttcgc gaggaacacc atcggcaata tggtatttct cgatcagttt
    24661 gtgctgttcc agatagaact gattcaccgt cttcagccag ttgcgcgcga tttcatcacc
    24721 cagaaggtca tcgccgtaca tcttaaatcc ctgaattgcc atccattgta acggtgccca
    24781 gccgttgggt ttatcccact gttcaccggt ttcgtactcg cttgccagaa tcccgccagg
    24841 tgtcagtaac cggctgcgca cggcgtttgc cagacgatcg gcctgttcat ggttcgccat
    24901 accgacatag agcggcacaa tggcggcagc ggaaaacagc gccagttgct cgcgtcgcca
    24961 gtcgtaatcg cggtagatgc cgttttcatc atcccagagg taacggttta ccgcatcgcg
    25021 acgggcactg gccttctggc ggaacagtgc ttctgtctct ttctcgcctt tcagcgccga
    25081 gatgttggcg atggcgctct ccagtttaaa caggaaggca ttcagatcga tggggatgaa
    25141 ctgggtggta cgaatgctcg ccagacgacc agtatcacgc agccaacggg aagagtaatc
    25201 ccaaccggag gccgcccccg cacgtaaatc gcggtacacc tcgttaggcg ggcgaccaga
    25261 atgtttcgcg gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt cgcgatcgtc
    25321 ccagtaacgg ttgagcagcg agccgtccgg catccgcaca acatggcgat aggcctgatt
    25381 agggatcaac gattctgcac cgtccatcca gaaggcatat tccattttca ggtggtcgag
    25441 atagcggcgc gcgccgcgta caccatcttc ttcaaacaac tccaccatca gcgcaaaaac
    25501 cggtggttgc gagcggctca aataataggt gcggttgccg ttggggatgt ggccgtagtt
    25561 ttcgatcatc caggcgaagt tatcggccat gcatttcagc aaatcttccc gaccactttc
    25621 cgccagcccc agcatggtga aataggaatc ccaatagtag gtttcgctaa aacggccgcc
    25681 cgggacaata tatgactgcg gcagcgccag cagagaagac cacggaatgt gatcctgtgg
    25741 ttcacgggtt agcaccggcc acagctggtc gatatgctct ttcagggaat tttgcgggtc
    25801 cgatacatac tcgctggagt agacctccgg cagccagaag tggttttcaa caaacttgcg
    25861 caagtcaaaa tcacgatgac ggcgcacttt acggtagcgg attaaaatat ccagcgggtc
    25921 cattttgggt gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg ccgactgaac
    25981 atgctcgaac agttcgagat agcgatcggc aggcgtcagc gcatcagagg cgggcagccc
    26041 ttcaatcatc tcgggttccg gttctgcctc gatcatctca tccagtttta attcatacgg
    26101 gtccagctca tagcagagat cgacctcgat catcagttcg tctggattag ggttttgaat
    26161 tttctgattg agcataagga tccgacctcc gaaattcgtc gctaattatc gggcttttct
    26221 ttaagggtag tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga tcacgtttat
    26281 taacctgcca aaaaatataa cgcactaata tcaatggaat ttataaaaat acggatttcg
    26341 tgttgttttt cattttcaga acattcaatc ctgctctcgc cgcaatcatg atatgccatt
    26401 cctgatctgg gtaataggca ctggctatct tattgatagt ttatattcat gtaattgatt
    26461 gattcctatc aaaaagcata tctccctgtt ttttatgatg gctttgtctc gcagagacgt
    26521 ttgcagcatt ctggaaaatt ttctgggtca caataactca ccaccgtgag ggaagaataa
    26581 tgaaaatggt ctcacgtatt accgcgatcg gcctggctgg cgtcgcgatt tgctatttag
    26641 ggttatctgg ttatgtgtgg taccacgata ataaacgcag taaacaggcc gatgttcagg
    26701 catctgctgt cagtgaaaat aataaggttt taggctttct ccgcgaaaaa ggatgcgatt
    26761 attgccatac gccttcggca gaattacctg cctattatta tattcccggc gcgaaacagt
    26821 tgatggatta cgacataaag cttggatata aatcttttaa cctcgaggcc gtgcgtgcag
    26881 ctctgctggc taataaaccc gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc
    26941 agtatgaaac tatgccgcca acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg
    27001 atgaagagcg ggcggaaata ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca
    27061 gcaatgatac tgctccggag catcgcaatg aaccggtgca gcccatcccg caaaaactgc
    27121 ctaccgatgc gcaaaaagtg gcgttgggtt ttgcgctgta tcacgatccc cgtttatcgg
    27181 ctgatagcac catttcatgc gctcattgcc atgcgttgaa tgcggggggc gtcgatggca
    27241 gaaaaacatc gattggtgtt ggtggcgcag ttgggccgat taacgcgccg acggtattta
    27301 actcagtatt taacgttgag cagttctggg atggtcgtgc ggcaacattg caggatcagg
    27361 ctggtggacc gccgttgaac ccgattgaaa tggcgtcgaa atcctgggac gaaattattg
    27421 ctaagctgga aaaagatccg cagcttaaag cgcagttcct cgaagtctat ccgcaaggtt
    27481 tcagtggcga aaatattact gatgccattg ctgaatttga gaaaacatta attacgccgg
    27541 attccccatt tgatagatgg ttgcgtggag atgagaatgc tctgacggcg caacagaaaa
    27601 aaggctatca attatttaaa gataataaat gtgcaacttg tcatggtggt attattctcg
    27661 gcggacgttc ctttgaaccg ttggggctga aaaaagactt taactttggt gaaattacgg
    27721 cggcggatat tggtcgtatg aatgttacta aagaagagcg tgataaattg cgtcagaaag
    27781 tacccggttt acgtaacgtt gctttaacgg caccgtactt ccatcgcggt gacgtgccga
    27841 cgctggacgg ggcggtgaaa ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg
    27901 aggatgtgga tgatatcgtc gctttcctgc acagtctgaa cggggtttac acgccgtata
    27961 tgcaggataa acaataatta atttgatcgc ccgaacagga gtg
//
LOCUS       DAZGJT010000060        27397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZGJT010000060 DAZGJT010000000
VERSION     DAZGJT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.060"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..210
                     /locus_tag="ACHKFK_002817"
     CDS             <1..210
                     /locus_tag="ACHKFK_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053111.1"
                     /translation="HFRTLSNRSLYFTLTQPPLIATIKDDVFCTLHDYYNHNNETKST
                     IDNYISKIQDNHNTPWILNNESVQK"
     gene            complement(1096..1224)
                     /locus_tag="ACHKFK_002818"
     CDS             complement(1096..1224)
                     /locus_tag="ACHKFK_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9053112.1"
                     /translation="MPQKTIIVSILCLTMLLTVWMLHASPCEFRLNIMWSEFAAFL"
     gene            1294..1593
                     /locus_tag="ACHKFK_002819"
     CDS             1294..1593
                     /locus_tag="ACHKFK_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053113.1"
                     /translation="MKNEAEKAMWSCKTSDTPWTFSRLLKKAICAVLPDSGPHTVHRV
                     NKKENNYLTSVLSDICDSNEKDSNEAMFNPFHPLYRDHHPIDSREWSGSPYKTPE"
     gene            complement(1640..3142)
                     /locus_tag="ACHKFK_002820"
     CDS             complement(1640..3142)
                     /locus_tag="ACHKFK_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006858964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraG N-terminal
                     domain-containing protein"
                     /protein_id="HFO9053114.1"
                     /translation="MTTNNYLEYFLTLLGWLVNNGLWDLLIGTGLFALPLAFKVIGIW
                     LKVREEGADEGNKGMLSLPRIENALYGAFFVMVASCVPLVQVTLDTLKFDRSRAQTCG
                     VWTPKAPGDTGYQGIISSLGDKTAAAPIWWVVVHKLSKGVTQAAVASIPCRPDLRQLR
                     FEVQRTFIANRALADELQDFTNDCYSLAMYQWKQRDQGMTKDRKVLSDISWIGSSTFM
                     QGDYGTLQSRMPRAAFPWNDARDSGRPDTGRGGYPTCREWWNDSSVGLKKRVTEEVDE
                     GLWLRLSAAMKLLGNSQATYQEEIIKRLVSPANLTISQGGEVYRGYGGNADMTTMNAI
                     SRIGATAGSGVASLGAFPAFDAMRQALPMVQSILLMAVYIMIPLVLAFAAYEFSTLIT
                     LTFVVFALNFLTFWWELARWLDSWMITALYGSDTHSSLNMMGLQNTSDDIIMNLVMGT
                     MFLVLPAVWLGALSWAGVNIGQAISEGFNRAGHVTQQAGQHAGSFVKRIK"
     gene            complement(3146..3511)
                     /locus_tag="ACHKFK_002821"
     CDS             complement(3146..3511)
                     /locus_tag="ACHKFK_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053115.1"
                     /translation="MNKTVLMVPGSRAGLLVKGMKLLGKWGLTVIVIMASALLIADTG
                     MRHAQEVDALRLWFHETRFGWLGWRFCIYCGLVWGCRKIWRAPGFRAEYRLRFARICM
                     VSALFFLLCEYAIFSAAGD"
     gene            complement(3504..4934)
                     /locus_tag="ACHKFK_002822"
     CDS             complement(3504..4934)
                     /locus_tag="ACHKFK_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO9053116.1"
                     /translation="MTMKVKTLVMMVMMVMLSCSTPVMADSILSLLKLNDSTFGYGKS
                     VDGAVSDKLFYTLGGGSVISQPATRSNMQKLGLDLGWSSDLMCGNFDLKTTVGNQLNG
                     ITDGFKNLMGDVIQGATGAVSSLPAMVIQRANPGLYDMITNGVLQANVAFDKAQLNCQ
                     NMAKRMMDFSDRSKWTQAAMMEEYKKVVNTSDGDAVKGDDAGRQATGKEGQKWIGGQQ
                     RGGAGQPAIRPVHDMTAAGFNMMNSLPVTSTSGVGAGSCNGSACEKFRNAEEAASAVV
                     KVLGDRSMRTCTDAKECTSGDSDQQPGTAVAGTGFAPMLEEATRINTEQLVRLVNGQD
                     KPTAENLAKLKTGSLAVSAGVIHALRRDPDNMSLTSRLAGELAMADTVETALVMRRML
                     LTGMSEPYAAAQPAALEEGDRRIASLDREIIALKSEMELKRDLARNSVLTIIERDNER
                     VSNNPMIQQTDNADSRVRSLEVPENE"
     gene            complement(4946..5920)
                     /locus_tag="ACHKFK_002823"
     CDS             complement(4946..5920)
                     /locus_tag="ACHKFK_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03756 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053117.1"
                     /translation="MMSRSFRLRAGALLLSVAALPLASASLNTASIVASAAAPDCISW
                     RVSGICYWLYCSGFSCKVRTSVKVTHYIPEVVVSTYTAPGGNPWKEMALVSQTAGGVE
                     NSITKGLAGLSAGGGNPADMKAQGERRSAIRFKYADALGHPATCLIGGMVPGFSCDSP
                     VTPAMPYFLSTLDSLVWRTGMPESLYPEALIPGKREIGSQAEENLWGSVYPRSGFVMQ
                     TDDDKAAAVVAQRVADIISRTGEPHVYKSIQTRKKDGFWPPDPVEENTGTRNHKWQRL
                     TPSVSRSCAVFPDGDHQTAEDGNAAFALWQPYSCCKKRGQRFLGSTNF"
     gene            complement(5917..6315)
                     /locus_tag="ACHKFK_002824"
     CDS             complement(5917..6315)
                     /locus_tag="ACHKFK_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03757 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053118.1"
                     /translation="MHRIIFSSLFFLPTAVSADITLYTDSQHPPVNPPSDVRVILLDG
                     PEQLQARFFGALPADAGEAEERVRARMQSPEWGAMQAELAERYREVAHAWSLGVKKYP
                     AVVFDDRDVVYGTTDVAEAAQLRAQEGVQP"
     gene            complement(6542..8632)
                     /gene="iha"
                     /locus_tag="ACHKFK_002825"
     CDS             complement(6542..8632)
                     /gene="iha"
                     /locus_tag="ACHKFK_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO9053119.1"
                     /translation="MRITTLASVIIPCLGFSASSMAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSRQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWTSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNIGARLDWKATEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHSFTFGTWKSYLNWNETENKGRELVQSVLKRDKWELAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGASTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(9023..9409)
                     /locus_tag="ACHKFK_002826"
     CDS             complement(9023..9409)
                     /locus_tag="ACHKFK_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9053120.1"
                     /translation="MMYLKYPEPELQTAVHPGGVVFFLPPQGKPGVTEITQPHLLRLR
                     SHLNRRLGRLRRVVCQPQRVGMNSSVAVTLEGDTGKVYLLVSVGGHECWPSEEEYTHL
                     RWYIQVVDAADLFYLLLWLNGMEAEK"
     gene            complement(9406..12222)
                     /locus_tag="ACHKFK_002827"
     CDS             complement(9406..12222)
                     /locus_tag="ACHKFK_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer ATPase"
                     /protein_id="HFO9053121.1"
                     /translation="MAFTLFKCRNVTTPSETETAGQGFVPPVPPGAQPLKRPGQMTQE
                     EEVRVYHAGPSVIDFLPWVEYLDEAQCLLLDDGMSVGAVYDVTPAATEGRTEERLEQI
                     RDMVEDALQDSFDEYDTHPWVVQFFCQDENDVDAYLDYLKGYVKPHAQGTAFTEAWLG
                     EMECHLRGIARPEGFFTDTLVTGQPWRGQQRRTRMVIYRRIAKNNSDPMPPVAMLNQV
                     CDRVVSALGSAGVRCHRMNGLQVHSWLLRLFNPAPSWISREDLYRMASRADPRENDAD
                     VMPVMTDFAESLWFNPPHSDPENGVWWIDDLPHCAVVVEKLRTPPEPGAITGEMSRGE
                     KTVNALMDTFPEGTVLCMTIVVQPQDTLEERFTRLSKNAVGENTESIRARQDVKVVKE
                     LLGNRHKLYRAGITFLLRAGDMDSLKRKRVELTTVLLGAGLQPVRPEFDVGPLNSWLR
                     ALPMCFDPDMDRKQWHTRLMWVQHLAGLLPVTGRETGTGHPGFSFFNRGGDVLTFDPL
                     NKLDRTQNAHLLLFGPTGAGKSATLCASLSQVMAVHRPRLFIAEAGNSFGLLADYFES
                     LGLSVNKISVKPGSGVTLPPFADAHKLVEEGQTLQSVDEHSLPDLDGDEEEEKRDILG
                     EMEISARMMITGGDPKEEASLKRADRAMIREALLMATHATYREGRQMLPEDLQVALRE
                     ISCDAGRNEVRRAKAAEMAESLGMFTQKGSFEAELFNREGELWPEADVTLIDLGHLAR
                     EGYEAQMALTMVSMTNMINNIAERDQYLGRDIVFAVDEAHIVTVNPLLSPYMTKVVKM
                     WRKLGAWLWLATQNLKDYPDISEKMLNMAEWWVCLTMPPEEVNNIARFKSLTEEQKAV
                     LMSASKLSGCYTEGVVLAKKIEALFRAVPPSLYLALGMTEKDEKAERRMLMKEHNCSE
                     LEAAFHVARKLDRLRGLSAVESAA"
     gene            complement(12222..12635)
                     /locus_tag="ACHKFK_002828"
     CDS             complement(12222..12635)
                     /locus_tag="ACHKFK_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03751 family conjugal transfer lipoprotein"
                     /protein_id="HFO9053122.1"
                     /translation="MKRLRLVMCIPLAALLLSGCSTSKEELLPAGDQTMLELWQGKTT
                     GGSQRHALAARETLWRPLTSDEKQQVHREGQSYSRTQESEISQQFPRLPNPDMVMYVF
                     PHLAGGTSPVPGYSTVFPFYSRTQYALPGERTEAM"
     gene            complement(12653..14131)
                     /locus_tag="ACHKFK_002829"
     CDS             complement(12653..14131)
                     /locus_tag="ACHKFK_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03752 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053123.1"
                     /translation="MQIKSNALVKFVVPAVVLVGLAVTMKSCKTQENVADRGEQARAL
                     SGLSSEELRALGVEGDTPEDTLRTLVARMNQVRDNQRTLEKQNSQLLKDNEQLRRRST
                     DVSGQVNDAVAGGLKTYEQKQRELQEQQHSLMSQIDAMKDQLSRAETLSRRQESDIPP
                     GLGLDAGGGSGVVISQGADGLMWVSPADQSQDDPLSSSGSKGGPRFPTSFLEDNALSR
                     QKAEYEQQVKGYSNEQQRATTQAVYTLPENSTLTGSRAMTALLGRVPINGTVTDPYPF
                     KILVGRDNLTANGIELPDVEGAIVSGTASGDWTLSCVRGQVNSITFVFADGTVRTLPS
                     PDVNGTTNSNQQAKGNGTGGIGWISDEDGIPCISGTRKSNASSYLPTIFALSAAGAAG
                     DALSEGQYTSQNNVNGITSTLTGSAGQAVLGKALSGGMNETADWVRQRYGQTFDAIYV
                     PPGKALAVHITRQLAIDYEDQGRRVRYDFALPGEANDHGGLD"
     gene            complement(14121..15029)
                     /locus_tag="ACHKFK_002830"
     CDS             complement(14121..15029)
                     /locus_tag="ACHKFK_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03749 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053124.1"
                     /translation="MKADIVCSLLRPAVMMMSLSVLSLTAQADELMKWERIPLNIPLK
                     VGQERVIFADKNVRVGFPPALNGKLRVQSTGGAVYLKADSAFPQTRVQLQDVESGEMI
                     LLDVTAGEKGTTEPVRIVYDGDVSTVSRAAGSDVQTVKSRSSNGKPGTDNSTQARRTE
                     PRYAEPLPVLLTRYAAQSLYAPLRTLEPVAGIRPVVPHLPRQITTVYPSEPLTVSPVA
                     AWAAGGRTVVALKLTSTVSRRIVLDPRALQGRFVAATFQHRWLGAAGTPEDTTMLYVV
                     TAGSPEKAFVEEPVTSRRGGEVTHAD"
     gene            complement(15026..15673)
                     /locus_tag="ACHKFK_002831"
     CDS             complement(15026..15673)
                     /locus_tag="ACHKFK_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4703 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053125.1"
                     /translation="MSRFRHALKDRDQHILTLRVFSGVLCGLLLMVTIGWMTAPDRLT
                     IHNPPDLRSGSTRPWWEVPPSTVYSFAFYIFQQLNAWPKNGEVDYPAKIHALSAYLTP
                     SCREILETDARRRKDAGELLDRVRVVYEIPEHGYGGRYNRDPVTVEGRDNWLVRLDLV
                     ADEYFHAEPVKRALIRYPMKVMRMEGDPERNPFGLVLDCYSSTPQRLEAVKGGGE"
     gene            complement(15670..16035)
                     /locus_tag="ACHKFK_002832"
     CDS             complement(15670..16035)
                     /locus_tag="ACHKFK_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03750 family conjugal transfer protein"
                     /protein_id="HFO9053126.1"
                     /translation="METIRFLPDRLNAEPTVFRGFTTPELGLAALTGFFTGLLWSLPF
                     VPVFGWVIIPTGALLTPLVLVWTGGVWISRLKRGKPDNWLWQRLEEKKAALGVGRSGL
                     IREARGWSLRRTRVRGGKS"
     gene            complement(16055..16393)
                     /locus_tag="ACHKFK_002833"
     CDS             complement(16055..16393)
                     /locus_tag="ACHKFK_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03745 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO9053127.1"
                     /translation="MARLAGMAMVTFLLGSLTPALADLPQIEPPSSGGGGGLFGQIKG
                     YFQDGIVLIGLVIAAAAFVKVAEAAVTTFSEVRDGKTGWGQFGAIIVVGVVLLVAVIW
                     LLGKSASIIL"
     gene            complement(16463..16705)
                     /locus_tag="ACHKFK_002834"
     CDS             complement(16463..16705)
                     /locus_tag="ACHKFK_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03758 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053128.1"
                     /translation="MALTSAQAAAFKVASGDVDLNVLHLSSLGLLIAVLFLWAAWGLS
                     DVWSGWRNGDVRESAFIWFTARTVLLLVASIWMFAG"
     gene            complement(16705..16989)
                     /locus_tag="ACHKFK_002835"
     CDS             complement(16705..16989)
                     /locus_tag="ACHKFK_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016190711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAQPRD family integrative conjugative element
                     protein"
                     /protein_id="HFO9053129.1"
                     /translation="MMLLVTLVLPVQAAEKDELATVMRQLDQVRAALDRARTASLQAT
                     PHDGRYFFDYPRAQADIQTMQEGIDRYLTPSRAQSRNGSAVTGEYRRERP"
     gene            complement(17996..18757)
                     /locus_tag="ACHKFK_002836"
     CDS             complement(17996..18757)
                     /locus_tag="ACHKFK_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03747 family integrating conjugative element
                     membrane protein"
                     /protein_id="HFO9053130.1"
                     /translation="MAEARRDAERSPQSRPPRQHGLLYNLVWGWPWALVGLILSSLLL
                     SLLIEYAGMTFFWPDEGAAHSRAVMNTELGWLSTEFTRSLLLSTPSVTVPGWISVAYQ
                     WLFVESGMQSWLQPQQAVTAVGRSVSQFSGELSQWLAYHLHDYLLATVYVTVVTMVRV
                     IILVLSVPLFVMVILVAVVEGLGRRDLRRYGAAYESSFVYHHAKKSIKPAIYIPCILY
                     LSWPTAVYPNLLIWPAAMLLGMAVTVTMASFKKYL"
     gene            complement(18738..20855)
                     /gene="traD"
                     /locus_tag="ACHKFK_002837"
     CDS             complement(18738..20855)
                     /gene="traD"
                     /locus_tag="ACHKFK_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO9053131.1"
                     /translation="MSNRYVIEALLRPAVEFNTAVVAATAAGVCVTAPWAVALAPSVS
                     YVTAAGFGVLAAVRFRQGMKIIRYRRNLRRLPRYVMSTRQIPVSRQRLFLGRGFRWTQ
                     KHTQRLQDTLRPEVAHYLLPGGFYRAARWLEMKTEHRLPGLGQWLRRDSPLNPVRPLP
                     PVGGNPALHGIEPDEQDVTLALGERVGHTIVYGTTRVGKTRLAELLVTQDIRRGEVTI
                     VFDPKGDADLLLRVWAEAHRAGRGDELYIFHLGWPEISARYNAVGRFGRVSEVASRVA
                     GQLSGEGNSAAFREFAWRFVNIIARALVALGERPDYMLIMRYVNNIADLYIRYAGKVI
                     RERLPGLEQIIANNQSVLSEEDVPRTMQNQPDAVRIWSIEMALSSEEGKKIYDPILDG
                     LRSAVRYDRTYFDKIVASLLPLLEKLTTGRIAELLAPDYLDMSDARPIFDWEQVIRKK
                     AVVYIGLDALSDSEVASAVGNSMFADLVSVAGHIYKHGMHAGLPAGGAGKNVVNLHCD
                     EFNELMGDEFIPLINKGGGAGMQVTAYTQTSSDIEARIGNAAKTAQVQGNFNNMIMLR
                     VRENRTAELLTTQLPQVEIYTKTLVSGHQDTADVSAGQDFTSSTQDRVGTVKVPLLEP
                     ADIVTLPKGQAFALLEGGQLWKIRMPLPAGDTDDVLMPESISRVAEAMRQNYHSGEGW
                     WNAPPSVQAIATEEGGDHGGSTS"
     gene            complement(20848..21636)
                     /locus_tag="ACHKFK_002838"
     CDS             complement(20848..21636)
                     /locus_tag="ACHKFK_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053132.1"
                     /translation="MNVSFTEQVQRNMPQWANEPLRAWDAAEFAAMSDFITEHYWTGQ
                     GSINVFRIVGTDHPQYAGMSWLELLERGKRMDINIPLVEKNPGYYTQAEQLHAGMSFV
                     STDGIHWYVSADGNHRSCLARFLFHLQGEERTQLHNVAQSIYHADRAFWSACREIHNL
                     ADVLSRHGVYLRLQTRRQSVSREDLACWKVDRFRTEAQLTVDDGRAGGHASAEYKALL
                     LNAADAWREVRRLQNRAEALSASPETDIPRSWWLRLLQRGRGHE"
     gene            complement(21650..22248)
                     /locus_tag="ACHKFK_002839"
                     /pseudo
     CDS             complement(21650..22248)
                     /locus_tag="ACHKFK_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946004.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(22248..22754)
                     /locus_tag="ACHKFK_002840"
     CDS             complement(22248..22754)
                     /locus_tag="ACHKFK_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO9053133.1"
                     /translation="MTAVWPLLLCAAQAGAELRVIADLGGSDASVFYEAINRQEGSST
                     PPPVTAPLPAQGETLLLPVTTQEMTPGEVTPRPLQLPGIGALFLVGDDALSRQWLQAN
                     AGALRERQAVGMVVSVADENSLRTLRELAPGVMMVPSSGSALARRLQLNHWPVLITDS
                     GISGVVTP"
     gene            complement(22775..23437)
                     /locus_tag="ACHKFK_002841"
     CDS             complement(22775..23437)
                     /locus_tag="ACHKFK_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase"
                     /protein_id="HFO9053134.1"
                     /translation="MARGSLLTMALLSMAVSSLCRAGAHDIPEGYVRVAAVHGVPAEV
                     LYAVSLTETVMAPSAIAGVMKKRDADFRLPSVARPWPWTINVAGKGYRYASRLEAWQA
                     LQVFLTRYPARRIDVGIAQVNLGWNGHRFSSTWAAFDPYISLNTAATILRECRRRHPV
                     SWLQAAGCYHYPAGGAPAVRYTAVVRQHLERLHRDAVKTAEQHDGLTAMHPRYIWTEP
                     RE"
     gene            complement(23416..24138)
                     /locus_tag="ACHKFK_002842"
     CDS             complement(23416..24138)
                     /locus_tag="ACHKFK_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006785960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03759 family integrating conjugative element
                     protein"
                     /protein_id="HFO9053135.1"
                     /translation="MKMKNLSPVLMLLISLQTVAAVRESAPEGTRTQASSQVSTTTQA
                     LQQQAAQWGVTEEDYRRYRALMEGPRGMQSPGLDPLTVLGIEARTDAERRQLAEKWVK
                     AEYERTEKELAFQREVNAAWKRLYPGVMPVSLGKGGGLLNDNGGRLALFVKAKDCGTC
                     DARLASVLSSGRQVDIYLVDSQGSDNLLRQWALEHDIPVDRVRSRHITLNHDAGRWLR
                     FGEGRMPVVLQQGADGWRVAAF"
     gene            complement(24143..24910)
                     /locus_tag="ACHKFK_002843"
     CDS             complement(24143..24910)
                     /locus_tag="ACHKFK_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053136.1"
                     /translation="MSEKKQKTLPSVHSSPLDGWTCVSPMLSVGFVLTVLVATILAVC
                     GLYRYSGEQDERIAALEGQMKTYSDALALSGSELEPLRSRLAETKKQLKSVTERLTLL
                     EQRPVLTEEEHARTGQMAASLASLQATLAQQGETLRAYDLRLEKLEKAPFTPVSPSSA
                     KGTGTVKSTPAVTPRQKKGSSGISVISRSAPFVLTGTEQRGGRTYAAVAPRGFTRLSD
                     VTLLGEGESTAGWMLERAGRGEASFRVNGRQVRVMVE"
     gene            complement(24907..25485)
                     /locus_tag="ACHKFK_002844"
     CDS             complement(24907..25485)
                     /locus_tag="ACHKFK_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilL N-terminal domain-containing protein"
                     /protein_id="HFO9053137.1"
                     /translation="MKTSLLLSVLSACVLLTGCQGQQKLLQRDAAVPVPSVTVSNNVR
                     PASPDMYAGTTVPEVVRYDRYLLVNTHPDAVQRDPLSQVIDIRIPASLKPTVADAMRY
                     ALKQSGYTLCATGPANGVLYRQALPAVQYQTGPLRLRTALQMMAGPAWQLEVDDVQRV
                     VCHSLREGYQLPPGQLAPVPAGVRGAGAELKK"
     gene            complement(25639..26007)
                     /locus_tag="ACHKFK_002845"
     CDS             complement(25639..26007)
                     /locus_tag="ACHKFK_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9053138.1"
                     /translation="MTDIELTGKSITLTDKYGSCVHQNIVLTYLMELYQMDFAELSEA
                     VSTHYPSHKGVIMTIAEQLEEKGLEKGRAEERSRLVLMMRQKGKSLEEIKDFLDLTDE
                     QLLQALDYVPVQRNGALNQD"
     gene            complement(26081..27004)
                     /locus_tag="ACHKFK_002846"
     CDS             complement(26081..27004)
                     /locus_tag="ACHKFK_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9053139.1"
                     /translation="MSEKVNKTSLIHDAAFRKTLKDPAAARDFLEQVLTPYQKSRCNL
                     DTIELESTTFVAESLRQSACDVLLSMKTNDGKDGYIYTLIEHQSSPDKFIPLRMMRYI
                     LAVMEQHIEEHKCAPVVIPVLFYHGAKRPYPYPMNWVDCLDDPVYGREIYGEQKPFSL
                     VDVSTLTDDEIEHYHRMAALMFTMKSGTSGDVIELIGKSITLTDKYGSSVHQNIVLTY
                     LMELYQMDFAELSEAVSTHYPIHKGVIMTIAEQLEEKGRAEERSRLVLMMRQKGKSLE
                     EIKDFLDLTDEQLLQALDYVPVQRNGTLNQD"
     gene            complement(27051..>27397)
                     /gene="ssb"
                     /locus_tag="ACHKFK_002847"
     CDS             complement(27051..>27397)
                     /gene="ssb"
                     /locus_tag="ACHKFK_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9053140.1"
                     /translation="YLRKGSKVYIEGQLKTRKWFDQQNGVDRYSTEVVVGVNGVMQML
                     GSLRQQGTAGNGASAPAQGGWGQPPKTAGQAGRAPQTTSTGNGAPPMDFEDDIPFMGL
                     GYGMERRIIHAM"
BASE COUNT         6395 a         8574 c         6434 g         5994 t
ORIGIN      
        1 catttcagga cactaagcaa tcgttcttta tatttcaccc tgacacaacc cccattaatc
       61 gccacaataa aagatgatgt attttgcact cttcatgatt actataacca caataatgaa
      121 accaaatcca ccattgacaa ttacatatca aaaatacagg ataatcacaa tacgccatgg
      181 atattaaata acgaaagcgt tcagaaataa aaaacacaga gacaacgatc aggataacac
      241 aaaacagcat aatcacatga accggaaatt ctatttcaaa aatatcctgt ggggaatgtg
      301 ccccattata acatccatat tacatcttat atataacaca ccaacagaaa cgtcgataaa
      361 agttgactta tttctgatac taactggcct ggcccttcct tgttcaatat acctgacaaa
      421 aacaatcaca ctaaaataca catccatatc attctggaaa aaaccttttt tctggtctta
      481 tggtatatat cattttcatc ttgtttatat aatgatatgt atggttttag caataccttc
      541 tgcaattatt gttttactct ttcactttta tcaaaacatc accagaaaca ggaaatgaca
      601 gtcgcaataa tgaacataaa aagacaaacc aggatggcag taaatcaata aagtaccatt
      661 tcatatgaat catcagaaaa acggtggacg cgatatccgg cacggggcaa aaacacagca
      721 acaaaaaata aaccggatat cgcgcccggt acttttctaa ctcaacttca ggcattgtgc
      781 aagttttgat cgtcggtgga ttcagagcca tgccccattg attcaggctg aagactcaca
      841 tatcattgaa gagactagaa tctcattgct gtgaaattcc ttacagaatc ttcagcccca
      901 ttttatgggg ctttttttgg ctaaacccac gctgtcacca ggccttcagt atgcgaataa
      961 aaaagcccgc agtttttaca ctgcaggctt caggttttct ttgtgatttg catgcaaggg
     1021 tgcgactttc gaccgaacct tctgcaaccg gatagcgggg gctttcaccc ccgcttcggt
     1081 ttcttacggt ttacactaca agaacgccgc aaattctgac cacatgatgt taagtctgaa
     1141 ttcacacggt gacgcgtgta acatccagac ggtcaacaac atggttaagc ataaaatgct
     1201 cacgatgatc gttttttgcg gcatatattg aacatttatc atacagcacg tatgatatca
     1261 ctgtgataac tacctgctct ggggagctgc gctatgaaaa acgaagcaga aaaagctatg
     1321 tggagttgta aaacctctga cacaccatgg actttcagcc gcctgctgaa aaaggctatc
     1381 tgtgcggttt taccggattc tggtccacac acggttcatc gtgtcaataa gaaagaaaac
     1441 aattatttaa catctgtttt atctgatata tgcgattcaa atgaaaaaga ttcgaatgaa
     1501 gcgatgttca atccctttca tcctctttat cgagatcatc atcctattga tagtagagaa
     1561 tggagtggta gcccttacaa aacgccagaa taaaaatatg gcgatattca tgacaaatat
     1621 cgccataaaa aacaggtagt tattttattc ttttaacaaa acttccagca tgctggcctg
     1681 cttgttgtgt aacatgccct gctcgattga atccctcaga aatagcctgt ccgatattaa
     1741 ctccagccca ggataatgcc cccagccaca ccgccggtaa tacgagaaac atcgtgccca
     1801 tcaccagatt catgatgata tcgtccgacg tgttctgcag ccccatcata ttcagactcg
     1861 aatgtgtatc tgaaccatac aacgccgtta tcatccagct gtccagccac cgcgccagtt
     1921 cccaccagaa cgtcagaaag ttcagcgcaa acacgacaaa tgtcagcgtg atcagcgtac
     1981 tgaactcata cgccgcaaat gccagtacca acgggatcat gatatacacc gccatcaaca
     2041 gaatactctg caccatcggc agtgcctgcc gcatggcatc aaacgccgga aacgccccca
     2101 gactggcgac accggaacct gccgttgcgc caatgcgtga aatggcattc atcgtggtca
     2161 tgtctgcatt accaccatat cccctataca cttctccccc ctgagaaatc gtcaggttag
     2221 ccggactgac cagccgttta ataatctcct cctgataagt cgcctggctg ttccccagaa
     2281 gcttcatcgc tgccgaaaga cgcagccaca gcccttcatc aacttcctcc gtgacccgct
     2341 ttttcagccc cacactgctg tcgttccacc actcccggca cgttgggtag cctccgcgcc
     2401 ccgtatccgg acgaccgctg tcacgggcgt cattccacgg aaacgccgcc cttggcattc
     2461 tggactgcag cgtgccgtaa tccccctgca tgaacgtgct gctcccaatc cagctgatat
     2521 ccgacagcac ctttctgtcc ttcgtcatac cctggtcacg ctgtttccac tggtacatcg
     2581 ccagcgagta gcagtcattg gtgaaatcct gcaactcatc ggccagcgcc ctgttcgcaa
     2641 tgaacgtcct ctgcacttca aaacgcagtt gccgcagatc aggcctgcag ggaatggacg
     2701 ccacggccgc ctgtgtcacc cctttggaaa gcttgtgcac aacaacccac cagatcgggg
     2761 ccgccgccgt cttatctccc aggctgctga taatcccctg ataaccggtg tccccggggg
     2821 ctttcggcgt ccacacacca caggtctggg cccggctgcg atcaaacttc agggtatcca
     2881 gcgtcacctg aaccagcggt acacaggaag ccaccatcac aaaaaacgca ccatacaatg
     2941 cattctcgat gcggggtaac gagagcattc ccttgttgcc ttcgtccgcc ccctcttccc
     3001 tcaccttcag ccagataccg atcactttga aagccagagg aagcgcaaac agtcccgtgc
     3061 caatcagcaa atcccacaga ccgttattga ccagccagcc cagcagggtc agaaaatatt
     3121 ccagatagtt attggtcgtc actgctcagt cccccgcggc ggaaaaaatc gcgtattcgc
     3181 acagcaggaa aaacagcgca ctgaccatac aaatccgagc aaaacgcaga cggtactctg
     3241 cccggaatcc cggtgcacgc cagattttcc ggcatcccca tacaagcccg cagtaaatac
     3301 aaaaccgcca gcccagccat ccgaaacggg tttcgtggaa ccagagcctc agcgcatcca
     3361 cctcctgcgc atgacgcata cctgtatccg caatcaggag cgctgacgcc atgatgacaa
     3421 tcactgtcag cccccatttc cccagcagtt tcatcccttt cacaagcagc ccggcccggc
     3481 tcccgggcac catcagcacg gttttattca ttttcaggga cctccagtga acggacacgg
     3541 ctgtcagcgt tgtcggtctg ctgaatcatc gggttattac tcacgcgttc attgtcacgc
     3601 tcaatgatgg tcagcacact gttacgcgcc agatcccgtt tcagctccat ctcacttttc
     3661 agggcgatga tctccctgtc cagagaggca atacgacgat cgccttcttc cagcgcagca
     3721 ggctgtgcag ccgcatacgg ttcagacatt cctgtgagca gcattctgcg catcaccagt
     3781 gccgtttcca ccgtatcagc catcgccagt tcaccggcca gacgggaggt cagggacata
     3841 ttgtccggat cgcgacgcag cgcatgtatc accccggcag ataccgccag actgccggtc
     3901 ttcagttttg ccagattctc tgccgtgggt ttatcctgcc cgttcaccag gcgaaccagc
     3961 tgctccgtat ttatgcgtgt cgcctcctca agcatcggcg caaaccctgt tccggccacc
     4021 gccgttcccg gctgctggtc ggagtcacca ctggtacatt ccttcgcatc cgtacaggtg
     4081 cgcattgaac gatcacccag taccttcacc acagcgctgg ccgcctcctc tgcattcctg
     4141 aatttctcac aggcactgcc attacagctt cccgcaccaa caccggatgt actggtcacc
     4201 ggcagactgt tcatcatgtt aaaccccgcg gccgtcatgt catgaaccgg acggatggcg
     4261 ggctgaccgg caccaccacg ttgctgacca ccaatccatt tctgcccctc tttccccgtt
     4321 gcctggcgtc cggcgtcatc acctttaacg gcatcaccgt cactggtatt aacaaccttt
     4381 ttgtactcct ccatcatcgc ggcctgcgtc cacttgctgc gatcggaaaa atccatcatc
     4441 cgtttcgcca tgttctggca gttcagttgt gccttgtcga acgccacgtt tgcctgtaac
     4501 acgccattcg tgatcatgtc gtagaggccg ggattcgcac gctgtatcac cattgcagga
     4561 aggctggaga ccgcaccggt cgccccctga atcacatccc ccatcaggtt tttaaagccg
     4621 tcagtgatcc cgttgagctg gtttcctact gtggttttca gatcgaagtt cccgcacatc
     4681 agatcgctgc tccagcccag gtccagcccg agcttctgca tgttactgcg cgtcgccggc
     4741 tgggaaataa cggagccacc gccaagggtg taaaacagct tgtccgacac cgcaccatcc
     4801 accgattttc cgtaaccaaa cgtgctgtca ttcagtttta gcagcgagag aatgctgtct
     4861 gccatgaccg gcgtactgca ggacagcatc accatcatca ccatcatcac cagagtcttt
     4921 accttcatcg tcatcatccg tctccttaaa aattcgtact gccaaggaac cgctgccccc
     4981 gcttcttgca gcaactgtag ggctgccaga gcgcaaaagc ggcattacca tcctccgcgg
     5041 tctgatggtc tccgtcagga aacacggcac aggagcggga gacggagggc gtcagtctct
     5101 gccacttgtg gttacgggtg cccgtatttt cctcaaccgg atccggcggc cagaaaccat
     5161 cctttttccg ggtctgtatt gatttataaa catgcggctc accggtccgg ctgatgatgt
     5221 cagccacccg ctgggcaacc acggccgctg ccttgtcatc atcggtctgc atcacaaaac
     5281 ctgaacgggg ataaacactg ccccacaggt tttcttcagc ctgactgccg atttcacgtt
     5341 taccgggaat gagggcttcc ggatacaggg attcaggcat tccggtccgc cagacgagac
     5401 tgtccagggt actcaggaaa taaggcattg ccggtgtcac cggcgaatcg cagctgaatc
     5461 ccggcaccat tccgccaata agacacgttg ccggatgccc cagcgcgtcg gcatatttaa
     5521 aacgaatggc cgaacgacgc tctccctgag ctttcatatc cgcaggattt cccccaccgg
     5581 cactcaaccc cgccaggcct ttcgtaatag aattctccac ccctccggca gtctgactga
     5641 ccagcgccat ctctttccag ggattaccac ccggtgcggt gtaggtggat accaccactt
     5701 ccggaatata atgtgtcact ttcaccgatg ttctgacctt acagctgaac ccggagcagt
     5761 acagccagta gcaaattccg ctgacccgcc agctgataca atccggtgct gccgcactgg
     5821 ccacaatact ggctgtattc agtgaggctg acgccagagg cagagctgca acactcagca
     5881 gcagggcccc ggcacgcaga cgaaatgacc ggctcatcat ggctgaactc cttcctgtgc
     5941 tctcagttgt gcggcctctg ccacatccgt ggtaccgtaa accacgtcac gatcatcaaa
     6001 gaccacggcc ggatatttct tcacgcccag tgaccatgca tgcgccacct cccggtaacg
     6061 ctcagccagc tccgcctgca tcgcccccca ctccggggac tgcatacgcg ctctgactct
     6121 ttcttcggcc tcaccggcat cggcgggcaa tgcaccaaaa aaacgagcct gcagttgttc
     6181 aggaccatcc agaaggatca cccgcacgtc tgaaggcggg tttacaggag gatgttgaga
     6241 atcggtataa agggttatgt cggctgacac cgccgtcggc aggaaaaaca gggaactgaa
     6301 tataatccgg tgcatggtac gcctccgtca gacaatatct gaatgacagc gtgcgcagat
     6361 accagactaa tagcagcaat aaattaaaaa cgcgaggata aaaattaata tgaagatgca
     6421 tggtgcacaa aacggtatct gaacagaaat cagacaccgg gaatcatata acgtagccac
     6481 tgaacatcca ggagctacag tggcaacgta tgctaccgtc agtgatacgg ttttgtgatt
     6541 atcagaactg atagttcagt gacatccagt aatttcgctc aggtatcaca tagcccgttg
     6601 tcgatgctcc cgtctggaag taatcaccgg cataaagcgt actcttacca gcactgtaca
     6661 ggctcacgtc actgtaatcc ttgttgagca ggttattcac cgcagcattc agcgtcaggg
     6721 catccgtcat cttccacgac agacctgcat ccaccaccgt ccaggctttc aggtattctc
     6781 ctttctcatc atacactttc ttctgtacag cactcagcga cgaataattc tgggtgaaac
     6841 gtggtgtttt cccgcgataa cgggcaccca gccatgatgc cacctcttcg gtgatctgcc
     6901 agttcagttt cgcattcacc atgtgttcag gggtataact cagcggcgca cctttgttat
     6961 caccatcacg ttgttcactt cgggtccagg tgtaattcag tgacagcgtg acatcctctg
     7021 accacagcgg caatgtgccg gcaaattcca caccgtgcaa ccgggccttt ccgctgttta
     7081 catagctatt ggtgttatca tttatggaat aagagacaat cttgttggag aagtcagtca
     7141 taaaacctgt gacattggca ttcagaccgg cggggttatc gtaatacacc ccagcctcat
     7201 aactgacgct ctcttccggc ttcaggtcgg ggttaccaag tagatttgtt tttccctgcc
     7261 cggacacacc actaatccct ttatgtagct gccccattct gggtgcctta tatcccgtgg
     7321 tcacaccgcc tttcagcgtc caggcatctg ccacatccca gaccagatat gcacgcggac
     7381 tgaagtgtcc cccgaattgc tcatgatgtt catagcggct gcccgcagtc agcgcaagtg
     7441 catccgtgag atgccattca tcctcagcaa ataccgacca gcttttctgc cggaaagttt
     7501 cacctgtgct ggcaaggaca actccgtctt tcatggacga gctctgaaac tcgcccccca
     7561 ccgtaaccag atgagattct cccagagggg taagcagtaa tgaattcagg ataaggttag
     7621 attccttaag ctcccgcggc tgaccggcaa gctcccattt gtcgcgcttc agtacacttt
     7681 gtacaagttc acgaccttta ttttccgtct cgttccagtt cagatacgat ttccaggtac
     7741 cgaaggtgaa agaatgatca tagccagctg aaattttgtt tcgctcatag cgcaaggtac
     7801 ggtcatatcc cccggtcaga ctccccagtt gcccgtcccg gttatcataa cgctgccggg
     7861 tggtatccat atcaaaccag agtacatcct gctccgtagc tttccagtca agacgcgcac
     7921 caatattata attctgtgac tccgtgggat aaggaatacg cgtggctgct gtgtcgctca
     7981 gtgatgtgac cgatgatccc tgacgctgct gtgtgctgcc ccggacctgc agactgacag
     8041 aatcatccac aaggggacca ctggtccaga aattaaactg gctgctgtta ccccatttgt
     8101 tgctttcctg cagattcagc cccgcattga cggaagagag ccatttgtct gcattctttc
     8161 tggtaatgat attcaccaca ccgcccatcg catcagagcc atacagtgtg gacatcggcc
     8221 ccctgataac ctcaatacgc tcaatggcgg ccagaggagg catgaacccg gtattcatgg
     8281 cagaaaaacc gttgggagtc acgtcactgc tcccgccctg acgaacacca tcaatcagta
     8341 tcagcgtgta actggcaggc attccacgga tgctgatttc cagcccaccg gttttacccg
     8401 taccactttc aacatccaca ccctctacag atctcagagc ctccgccaga tcgtggtact
     8461 gcctggactg caattcctcc tggctgatca cagagacact ggcggctgcg ttagtcagtt
     8521 ttttctcata gccggatgcc gagacaatca tcacatcctc tgcagcagcc atgctgctgg
     8581 ctgagaatcc gaggcaggga atgatgacag aagccagagt ggttattcgc atattactaa
     8641 tctccgatca tattaaccgg caagacaata cacaaataca aacaataatg agaataattt
     8701 atatttactt tttacaaaaa ctgaagattc ataataaaaa aaaagatata caacgactgc
     8761 agcaacacgc ctgttaacgg ctatctgtct gacagattcg attctgaatg taaaaccggt
     8821 tactgcagaa acgtatgctc acagccagtc ggccataaat gctctctgga ggggcacgag
     8881 tgaaatgatt cagtcaatga gttcttccgg tgaatacgga taaaaatatt gccgtaatgt
     8941 aataaacgtg ttaattaaaa aacgcgtgta ccggcaggta tgaattgatc ccgtaaccca
     9001 tctgccggtt acacgctggc acttattttt ctgcctccat accattcagc cacaacagta
     9061 gatagaacaa atcggccgca tccacaacct ggatatacca tctcagatgc gtatactcct
     9121 cctctgacgg ccagcattca tgaccaccca cggaaacaag cagatagacc tttccggtat
     9181 caccttccag cgtcaccgcc acgctgctgt tcatgccaac ccgctggggc tggcatacaa
     9241 cacgacgcag cctgcccaga cgacgattaa ggtgtgagcg cagccgcaga agatgcggct
     9301 gtgttatctc ggtcaccccg ggcttaccct gtggcggcaa aaaaaacaca acgccacccg
     9361 gatgtaccgc tgtctgcagc tccggctccg gatatttcag atacatcatg ccgcactctc
     9421 cacggcggaa agtccccgca gtctgtccag tttacgggcc acatggaacg cagcctccag
     9481 ttcgctgcag ttatgttcct tcatcagcat gcgccgctca gccttctcgt ccttctccgt
     9541 catccccagc gccagataaa gactcggtgg caccgcacgg aacagcgcct caatcttctt
     9601 cgccaggaca accccctccg tataacagcc ggacagtttg ctcgccgaca tcagcaccgc
     9661 cttctgctcc tccgtcaggg atttaaaacg ggcaatgttg ttcacctctt ccggcggcat
     9721 cgtcagacac acccaccatt cagccatgtt cagcattttc tctgatatat ccggatagtc
     9781 cttcaggttc tgcgtcgcaa gccacagcca ggctcccagc ttacgccaca ttttcaccac
     9841 cttcgtcata tagggcgaga gcagcggatt caccgtcacg atatgcgcct catccacggc
     9901 aaacacaata tcacgcccca gatactggtc acgttcagcg atgttgttaa tcatattggt
     9961 cattgacacc atcgtcagcg ccatctgcgc ctcatacccc tcacgggcca ggtgccccag
    10021 gtctatcagc gtcacatccg cttccggcca cagttcgcct tcacggttga acagctccgc
    10081 ttcaaaactc cctttctggg tgaacattcc cagtgattcc gccatctctg ccgcctttgc
    10141 ccggcgtacc tcgttacgtc ccgcatcaca ggagatttcc cggagtgcca cctgcaaatc
    10201 ttccggcagc atctgccgcc cttcccggta cgtcgcgtgt gtggccatca gcagcgcttc
    10261 acggatcatc gcccggtccg cacgtttgag tgaggcctct tccttcggat caccgccggt
    10321 gatcatcatc ctggcagaga tttccatttc tcccagaatg tcgcgtttct cctcctcttc
    10381 atcgccatcc agatcgggca gcgagtgctc atccacactc tgcagggtct gcccttcttc
    10441 aaccagctta tgcgcatccg caaagggcgg cagtgtcaca ccgctccccg gtttgacact
    10501 gattttgttc actgaaagcc cgagactttc aaagtaatcg gccagcagac caaacgagtt
    10561 acccgcctcg gcaataaaca accggggacg atgcacggcc atcacctgtg acagagaggc
    10621 acacagcgtg gcagacttcc ctgcccccgt gggcccgaac agcaacagat gggcattctg
    10681 ggtacgatcc agtttgttca ggggatcaaa cgtcaggaca tctcccccgc ggttaaaaaa
    10741 actgaagccg ggatgccctg tacctgtttc ccggccggtc accggcagaa gccctgcgag
    10801 atgctgtacc cacatcagac gtgtgtgcca ctgcttcctg tccatatccg gatcaaagca
    10861 cattggcagc gcacgcagcc agctgttcag cggccccaca tcaaactccg gtcgcaccgg
    10921 ctgcaggcct gcacccagca gaaccgtggt cagctccacc cgtttacgtt tcaggctgtc
    10981 catatcaccg gcccgcagaa ggaaggtgat ccccgcacga tagagtttat gccggttacc
    11041 cagcaactcc ttcaccacct tcacatcctg acgtgcccgg atggactccg tattctcacc
    11101 cacggcattt ttcgacagcc gggtaaaccg ctcctccagg gtgtcctgtg gctgtaccac
    11161 aatggtcatg cacagcaccg ttccttccgg aaacgtatcc atcagcgcat tcaccgtttt
    11221 ttctccccgg gacatctcac cggtaatggc tccgggctcc ggtggcgtac ggagcttctc
    11281 caccaccacg gcacaatgtg gcagatcatc aatccaccac accccgttct ccggatcgga
    11341 atggggcgga ttaaaccaca ggctttctgc aaagtccgtc atcaccggca taacatccgc
    11401 atcgttctca cggggatcag ctcttgaggc catccggtac aaatcctccc ggctaatcca
    11461 ggacggtgcc ggattaaata accggagtaa ccagctgtga acctgcagac cgttcatccg
    11521 gtgacagcgc accccggcgc tccccagtgc cgacacaacg cgatcgcaaa cctgattcag
    11581 catggccacc ggcggcatcg gatcactgtt gttcttcgcg atacgacgat aaatcaccat
    11641 acgggttcgc cgctgctgtc cccgccacgg ctgaccggta accagcgtgt ctgtaaaaaa
    11701 gccttccggg cgggcaattc cccgcaaatg gcattccatt tcgcccagcc aggcttccgt
    11761 aaacgccgtt ccctgcgcgt gaggtttcac ataccctttc agataatcaa gatacgcatc
    11821 cacgtcattt tcatcctggc agaagaactg caccacccag ggatgcgtgt catactcatc
    11881 aaagctgtcc tgcagtgcgt cctccaccat gtccctgatc tgttcaagac gttcctccgt
    11941 acgcccttcg gtcgccgccg gcgtcacgtc ataaacagcc cctaccgaca ttccgtcatc
    12001 cagcagcaga cactgtgcct catccaggta ctccacccag ggaagaaaat cgataactga
    12061 cggcccggca tgataaaccc ggacctcctc ttcctgcgtc atctgcccgg gacgtttcag
    12121 gggctgtgcc ccgggcggga caggcggtac aaaaccctgt cctgccgtct ccgtttctga
    12181 tggcgtggtg acattgcgac atttaaacag tgtgaatgcc attacatcgc ctccgtacgc
    12241 tcaccgggta acgcatactg cgttctgctg taaaaaggaa ataccgtgct gtatcccggc
    12301 accggtgacg tcccgccggc aagatgcggg aatacgtaca tcaccatgtc agggtttggc
    12361 agacggggaa actgctggct gatttcactt tcctgcgtcc tgctgtagct ctgcccctcc
    12421 ctgtgtacct gctgtttctc atctgacgtc aggggccgcc acagtgtctc cctggcggcc
    12481 agcgcatgtc gctggctccc ccccgttgtt ttcccctgcc agagttcaag catggtctga
    12541 tcgcctgccg gcagaagctc ctctttcgag gtggagcacc cggataacag cagggcagcc
    12601 agggggatac acatcactaa ccgtaatcgt ttcacaaacg tattctccct gttcagtcaa
    12661 gccccccgtg gtcgtttgct tcacccggca gggcgaagtc ataacgcaca cggcggcctt
    12721 gatcttcata atcaatagcc agttgtcggg taatatgtac cgccagcgct ttccccgggg
    12781 gaacataaat ggcatcaaac gtctgcccgt aacgctgcct cacccagtct gccgtctcat
    12841 tcattccacc ggacagcgcc ttaccaagta cagcctgccc cgcgctgccg gtcagcgtgg
    12901 aggtgatacc gttgacattg ttctgcgagg tatactgccc ctcactcagg gcatccccgg
    12961 ctgcaccggc ggctgacagt gcaaatatcg ttggcagata actcgacgca ttggatttac
    13021 gggtaccgct gatgcaggga ataccgtctt catcggagat ccagccaatc ccgccagtac
    13081 cattcccctt cgcctgctga ttgctgttgg tggtgccgtt gacatccggt gacggcagcg
    13141 tgcgcaccgt gccatcagca aaaacaaagg tgatactgtt cacctgcccg cgcacacagg
    13201 aaagcgtcca gtcaccactg gccgtgccgg agacaatcgc cccttccaca tccggcagct
    13261 caatgccgtt tgccgtgagg ttgtcacgcc cgaccaggat cttgaacggg taaggatccg
    13321 tcacagtgcc gttgatcggc acccgcccca gcagggccgt catcgcacga ctgcctgtca
    13381 gcgttgaatt ttccggcagc gtgtaaaccg cctgtgtggt ggctcgctgc tgttcattgc
    13441 tgtatccctt cacctgctgc tcatactcag ccttctgacg actcagtgcg ttgtcctcaa
    13501 ggaatgaggt cggaaaacga gggcctccct ttgaaccact gctgctcagg ggatcgtcct
    13561 gagactgatc ggccggactt acccacatca gcccgtcagc cccctgcgaa atcaccacgc
    13621 cactgccgcc gcccgcatca agcccgagcc ccggcgggat atcgctttcc tggcggcgac
    13681 tgagcgtctc tgcccggctc agttgatctt tcatggcgtc aatctgcgac atcagggaat
    13741 gctgctgctc ctgcagttcc cgctgcttct gctcgtaggt cttcagcccc cctgccacag
    13801 cgtcattcac ctgaccggac acatcagtgc tccgcctgcg cagttgctcg ttatccttaa
    13861 gaagttgcga gttctgtttc tccagcgttc tctgattgtc ccgcacctgg ttcatacggg
    13921 cgaccagcgt ccgtaatgtg tcttccggcg tgtcaccttc caccccaaga gcccgtagct
    13981 cttctgacga taatccggat aaggcccggg cctgctcccc gcggtcagca acattttcct
    14041 gcgttttgca gcttttcatc gtgactgcaa gccccaccag aacaacagcg ggcaccacaa
    14101 atttcaccag cgcgttggac ttaatctgca tgcgtcacct ccccgcccct gcgggacgtc
    14161 acaggctctt ccacaaacgc cttctccgga ctgcccgccg tcaccacata cagcatcgtg
    14221 gtatcttccg gcgtaccggc agcacccagc cagcgatgct ggaaagtcgc cgccacaaaa
    14281 cgcccctgaa gggcccgggg atcaagcaca atacgcctgc ttaccgtact ggtgagtttc
    14341 agggccacca ccgtccggcc accggctgcc catgccgcca ccggtgaaac agtcagcggc
    14401 tctgacggat aaaccgtggt gatctgccgg ggaagatgtg gcaccaccgg tctgatcccc
    14461 gccaccggtt caagggtgcg cagtggtgca tacagggact gtgccgcata ccgggtcaac
    14521 agcaccggca ggggctcagc ataacggggc tccgtgcgac gggcctgagt actattatcc
    14581 gtccccggct tcccgttgct gctgcgactt ttcacagtct gtacatcact cccggcagca
    14641 cggctgaccg tactcacgtc tccgtcatac acgatacgca cgggctccgt ggttcccttt
    14701 tcccccgcag tgacatccag caggatcatt tccccgcttt ccacatcctg cagctgaacc
    14761 cgggtctgcg gaaatgcact gtctgccttc aggtagaccg caccaccggt actctggacc
    14821 cgcagttttc cattcagcgc cggagggaag cccacccgca cattcttatc cgcaaaaatc
    14881 acccgctcct gccccacctt cagcgggata ttcagcggta tacgctccca cttcatcagt
    14941 tcatccgcct gtgccgtcag actcagcact gacaacgaca tcatcatgac ggcaggacgc
    15001 agcagactgc acacaatgtc ggctttcatt caccacctcc cttaacggct tccagacgct
    15061 gtggcgtgga gctgtagcag tccagcacaa gaccaaacgg attacgctcc ggatcgccct
    15121 ccatccgcat cactttcatc ggatagcgga tcagtgcccg tttgactggc tctgcatgaa
    15181 aatactcatc cgccaccagg tcgagacgga caagccagtt gtcccgcccc tccaccgtga
    15241 cagggtcacg gttgtaacgc ccgccgtaac cgtgctcagg gatctcatac accacacgaa
    15301 cacgatccag cagttctcct gcatccttac gacgacgggc atccgtctcc aggatctccc
    15361 ggcatgacgg tgtcagatag gccgacagtg catgtatttt cgccggataa tccacctcgc
    15421 catttttcgg ccaggcattc agttgctgaa aaatgtagaa cgcaaaggaa tagaccgttg
    15481 agggaggaac ttcccaccac ggacgcgtac tgccggaacg cagatccggc ggattatgaa
    15541 tggtcagccg gtcaggggcc gtcatccacc cgatagtgac cataagaaga agtccgcaca
    15601 gcacaccgct gaacaccctc agcgtcagaa tatgctgatc acgatccttc agggcatgac
    15661 gaaaacggct catgatttac cccctctgac acgggtacgg cgcagggacc agccccgggc
    15721 ctccctgata agcccggagc gtcccacgcc cagcgccgct tttttctcct caagacgctg
    15781 ccatagccag ttatccggtt tcccccgctt cagacggctg atccataccc ccccggtcca
    15841 gacaagaacc agcggggtca gcagggcgcc ggtgggtata atcacccacc cgaacaccgg
    15901 cacaaaagga agtgaccaca gcagaccggt gaagaacccg gtcagtgcag ccagccccag
    15961 ttccggggtg gtgaaccccc tgaacaccgt cggttcggcg ttcagacggt cgggcagaaa
    16021 acgaatggtc tccataacca gctcccactg ttgcttacag gatgatggac gcggatttac
    16081 caagcagcca gatgaccgcg accagcagta ccacccccac tacaataatg gccccgaact
    16141 gcccccagcc cgtcttgccg tcccgcacct ctgaaaacgt ggtaacggcc gcctccgcca
    16201 ctttcacaaa ggcagccgcg gcgatcacca gaccaatcag cacaatgcca tcctggaaat
    16261 aacctttgat ttgcccgaaa agtccgccac caccaccgga cgacggcggt tcaatctgcg
    16321 gcagatcggc cagtgccggt gtcagactgc ccagcagaaa cgtcaccatg gccattcccg
    16381 ccagacgcgc catcaggcgc agacggctga cagtaagccc ttttttacgc atagatttct
    16441 ccttaaaaag tcacaccatg aatcaacccg caaacatcca gatgctggca accagcagca
    16501 gaacagtgcg ggcagtaaac cagataaacg cagactccct gacgtcaccg tttcgccagc
    16561 cagaccagac atccgacagt ccccaggcag cccagagaaa gagaacggca atcagtaatc
    16621 ccagcgagga cagatgcagc acgttcagat ccacatctcc ggatgccacc ttaaaggccg
    16681 cagcctgtgc cgacgtcagt gccatcaggg acgctcccgc cggtattcac cggtcacggc
    16741 actgccgtta cgtgattgcg cccgggaggg cgtgagataa cggtcgatcc cctcctgcat
    16801 ggtctgaatg tcagcctgag cacgcggata gtcgaaaaaa taacgcccgt cgtggggcgt
    16861 ggcctgaagg gacgcagttc tggcccgatc aagcgccgca cggacctgat caagctggcg
    16921 catgaccgtc gccagctcat ccttttcagc ggcctgaacg ggcagaacca gggtcacgag
    16981 aagcatcatc ccggcggtcc ccgtcagacc ggccgttttc cgggataatg caggaagaga
    17041 aaacatacgc acctccgtca gaatcaatgg gtgcgtacag catgcgtaag gcagggggag
    17101 ggatcagcga taaactctat ttgcaaaact gaaaattttt acgggagaat acacgggtgt
    17161 ggataaaggc aaacaacccg gagctcctgg catgaacgat atcatcagta taattattct
    17221 tctggcagtc tatctcatcg gcctgatatt cattgtcctg attttcaatg acatagacat
    17281 aacatcgaaa aagagaaagt acgaaaaaaa ctgaaagaag cgataataaa tggcagttta
    17341 caaaataatg atatctattt actggccgaa cgctggtctg tcagtcatga acatatcaaa
    17401 agcgcactgc tgtcagtttt caatgatttt ctgactgata acaacttttc acatcacgtc
    17461 ccccgggtca gagaactgat tacatggtac gagaaatacg ccccattcac agatctccct
    17521 gaggatataa aacaacagtt acaaaatata atcaatttac ccgatgccag tgagtcagaa
    17581 gtgcagttat tagcctcctc acttcatgac atttataatt ctcgtcagaa aaaagagaag
    17641 aaggccagaa aaataaacta tgttctgggg gcggtcagca ttgccagttt ctgcatcacg
    17701 ctgttccagg cctgttcaaa taaattcatt ccatcctgaa tgatttattc ctgtttatta
    17761 aaacacggca tcactacact tactgtctta tatcattggc gctgacaacg ttgcccccca
    17821 tggatctatg acgtcgtcaa ggccatcacg atgttcattg ttttcgtcaa cattgttgtt
    17881 attccggcgc tggcattacc cagacactga cgcgcactga ccgtaagggc gtggcttgtc
    17941 tgccgacacc ctgccacaaa acaagttttg catcagggtg tccggcctct gtggactata
    18001 aatatttctt gaaggatgcc attgtcacgg tgacagccat acccagcagc atggccgccg
    18061 gccatatcag cagattcggg tacacggccg tcggccagga aagatagaga atacagggaa
    18121 tatatatcgc cggtttaatg gactttttcg catggtgata aacaaagctg gattcatacg
    18181 cggcaccata ccggcgcaga tcacgtctgc ccagcccctc aaccacggcc actaggatca
    18241 ccatcacgaa cagtggcacg gacagcacga gaataatcac cctgaccatc gtcaccaccg
    18301 tcacataaac cgttgccagc aggtaatcat gaagatgata tgccagccac tggctcagct
    18361 ctccgctgaa ctgcgacaca ctacgcccca ccgccgttac cgcctgctgc ggctgcagcc
    18421 atgactgcat accactttcc acaaacagcc actgatacgc cacggaaatc cagcccggta
    18481 ccgtcactga cggcgtggac agcagcaggc tgcgcgtgaa ctccgtcgac agccagccca
    18541 gctctgtatt catgacggca cgactgtgtg ccgccccctc atcaggccag aaaaacgtca
    18601 tacctgcata ctcaatcagc agactcagta acagtgagga gaggatcagc cccaccagag
    18661 cccatggcca gccccacacc agattgtaca gcaacccatg ctgacgggga gggcgggact
    18721 gcgggctgcg ttctgcatca cgacgtgctt ccgccatggt cacctccctc ctccgtcgct
    18781 atcgcctgta ctgaaggtgg agcattccac catccttcac cggagtgata attctgtcgc
    18841 atcgcctccg ccacccggga aatactctcc ggcatcagca catcatccgt atcacctgcc
    18901 ggcagcggca tccggatctt ccataactgt ccaccttcca gcagggcaaa tgcctgcccc
    18961 ttgggcagcg tgacaatatc tgcgggctcc agcaggggga ctttcaccgt cccgacgcga
    19021 tcctgggtac tggaggtgaa atcctgaccg gctgacacat ctgccgtatc ctgatgaccg
    19081 gagaccagcg tcttcgtgta aatctccacc tggggcagtt gtgtggtcag caattccgcc
    19141 gtccggttct ccctgacgcg cagcatgatc atgttgttaa agttcccctg aacctgcgcc
    19201 gttttggccg cattaccaat acgcgcttca atatccgatg acgtctgggt gtacgcggtc
    19261 acctgcattc cggcgccgcc ccccttgttg atgagcggaa tgaattcatc gcccatcagt
    19321 tcgttaaact catcacagtg caggttcacc acatttttac ccgccccgcc agccggcaga
    19381 ccggcatgca taccgtgttt gtatatatgc cctgccacac tcaccaggtc cgcaaacatc
    19441 gaatttccca cggcactggc cacctcactg tcactgagcg catcgaggcc gatataaacc
    19501 accgcctttt tgcggatcac ctgttcccag tcaaaaatgg ggcgcgcatc gctcatatcc
    19561 agatagtccg gcgccagcag ctctgcaatc ctgccggtgg tgagtttttc cagtaacggc
    19621 agcagggaag ccacgatttt gtcaaaatac gtacggtcat agcggacagc actgcgcagt
    19681 ccatccagaa tcggatcgta tattttttta ccttcctcac tgctcagtgc catttcaatc
    19741 gaccagatcc gcaccgcatc cggctggttc tgcatggtcc tgggcacatc ttcctcgctg
    19801 agaacactct ggttattcgc gataatctgc tccagtccgg gcagacgctc acggatcacc
    19861 ttccccgcat agcgaatgta gaggtccgcg atgttgttca cataccgcat aatcagcata
    19921 tagtccggac gctcaccgag ggccaccagg gcgcgggcga tgatattgac aaagcgccag
    19981 gcaaactccc ggaaagccgc cgaattacct tcaccggaca actgaccggc aacacgggaa
    20041 gccacttccg atacacgacc aaagcgtccc accgcattgt aacgggctga aatttcaggc
    20101 cagccaagat gaaagatata caactcatcc ccccgcccgg cccggtgcgc ttcagcccag
    20161 acccgcagca gcagatctgc atcccccttg ggatcaaaaa cgatggtcac ttctccccgc
    20221 cggatatcct gggtgaccag cagctctgcc agacgggttt tacccacacg cgtggtgccg
    20281 tagacaatgg tgtgccccac acgttcaccc agtgccagtg tcacatcctg ctcatccggt
    20341 tcaataccgt gcagggccgg attcccgccc accggtggca ggggacgaac cgggttcagc
    20401 ggagaatcac gacgaagcca ctgccccagc ccgggcagcc ggtgttccgt tttcatttca
    20461 agccagcgcg cagcccggta aaatcctccc ggcagcagat aatgcgccac ctccggccgc
    20521 agcgtatcct gcagacgctg ggtatgcttc tgtgtccagc gaaatccccg ccccagaaac
    20581 agtcgctgac ggctgacagg aatttgccgc gtgctcatca catagcgggg taaccgacgc
    20641 agattgcgtc ggtagcggat gattttcatg ccctggcgga aacggacagc cgccagaacg
    20701 ccaaatccgg cagctgtgac ataactgacc gaaggcgcaa gggccaccgc ccagggggct
    20761 gtcacacaga caccggccgc cgttgccgca accacggccg tattgaactc cacagcaggc
    20821 ctcagtaacg cctcaataac ataacggtta ctcatgtccg cgtcctcgct gtaaaagacg
    20881 cagccaccat gagcggggga tatctgtctc cggtgacgct gacaacgcct cagctctgtt
    20941 ctgcaatctc ctcacctccc gccatgcgtc tgctgcattc agtaacaatg ccttatactc
    21001 cgcagacgca tgtccgccag cccgtccgtc atccactgtc agctgagcct ctgttctgaa
    21061 acggtccact ttccagcagg ccaggtcttc gcgggaaaca ctctgccgcc gggtctgcag
    21121 acgaagatac acaccatgcc gggagagaac atcagccaga ttgtgaattt cccgacaggc
    21181 agaccagaac gcccggtctg cgtgataaat gctctgggcc acgttgtgaa gctgtgtgcg
    21241 ctcctctccc tgcaggtgaa acagaaagcg ggccagacaa ctgcggtgat taccatcagc
    21301 actgacatac cagtgaatgc catccgtgct gacaaaactc atcccggcat gcagttgttc
    21361 tgcctgcgtg taataccccg ggttcttctc caccaacggg atattgatat ccatacgctt
    21421 accgcgctcc agcaactcca gccagctcat tccggcatac tgtggatggt ctgtccccac
    21481 aatgcggaac acattgatgc tgccctgccc cgtccagtag tgttccgtaa taaaatccga
    21541 cattgccgca aactctgccg catcccatgc ccgtaaaggt tcattcgccc attgcggcat
    21601 attcctctgc acctgttcag taaatgaaac gttcatcccc gctctcctgt catcttcgct
    21661 tttctccggt gagttcaccg gtcagtaatc gcccgttcag cagtttcagc agacgctgtc
    21721 cgctaaccag ttcaatatcc ggccacagca caagccgtgc ccggctggcc ggtcccgtac
    21781 gcccggtgtg aacaaaaaat ccacgacaac cttcacgacg cagtaattca ccaaaatcat
    21841 cgatatgtga cggtcggatg gtgcggccat agcgtttggc ctgaatcagc caccgctcgc
    21901 cgtcaatcag cacctgtccg tccagccctc cgtcgccgct gtagcgggaa ttgcggataa
    21961 cccgcattcc cctgcgttca aacgccagaa gcagcagttc ttcaaacaca tacgggttaa
    22021 tttttcgcag gtatttcacg cctgcaccgc cctccggcaa cgacgacagt tgcgccagca
    22081 cacgctcagc cttgctccgg taatggcggt gacgccactc actggcgcgt tcacgacgcc
    22141 ggcggcgacc acacaggacg gaaaaaacag gaacaacaca gccaggacac acagcagaac
    22201 gccgtacggt atcttatccg gcacaatatc cgtcatttgc cgcagcatca cggtgtcacc
    22261 accccggata ttccgctgtc cgttatcagt accggccagt gattcagctg cagacggcgt
    22321 gccagcgctg accccgatga tggcaccatc atcacgccgg gcgccagctc acgcagcgtt
    22381 ctcaggctgt tttcatccgc cacgctgaca accataccga cagcctgccg ctcacggaga
    22441 gcgcccgcat tcgcctgcag ccactgacgg gaaagcgcat catctcccac cagaaacagc
    22501 gccccgatcc ccggcaactg cagtggtcgc ggcgtcacct cacccggcgt catttcctgc
    22561 gtggtcacgg gcagcaacag cgtctctccc tgcgccggca gaggcgccgt aaccggggga
    22621 ggcgtactgc tcccctcctg gcggttgatt gcctcataaa acacggaggc atcactgcct
    22681 ccaaggtccg caataaccct gagttccgcg ccagcctgag ccgcgcacag cagcaggggc
    22741 cacactgccg tcattacgta tcgtttcaca ggtatcactc ccggggttct gtccagatat
    22801 aacggggatg catggcggtc aggccgtcat gctgctctgc ggtcttcacc gcatcacgat
    22861 gaagacgctc aagatgttgt ctgaccactg cggtgtagcg tactgccgga gcaccacctg
    22921 ccggataatg ataacaaccg gcagcctgca gccagctgac aggatgacga cgacggcatt
    22981 ctctcaggat ggtcgctgcg gtgttcagac tgatgtatgg atcaaatgcc gcccatgtgg
    23041 aggagaaacg gtgcccgttc cagcccagat tgacctgagc aattcccacg tcaatccgcc
    23101 gggccggata ccgtgtcaga aaaacctgaa gtgcctgcca ggcttccagg cgactggcat
    23161 agcggtagcc cttacccgcc acattgatgg tccacggcca gggacgtgca acggacggaa
    23221 ggcggaaatc cgcatcccgt tttttcatca caccggcaat cgcagagggc gccatcaccg
    23281 tctctgtcag agagacggca tagagcactt ctgcaggcac accatgcacg gcagccacgc
    23341 gcacatagcc ctcagggata tcatgcgccc ccgcccggca gagactgctg acagccatgc
    23401 tcaacagcgc cattgtcaga aggctgccac gcgccatccg tccgctccct gctgcagtac
    23461 aaccggcatt ctgccttcac caaaacgcag ccagcgaccg gcatcatgat tcagggtaat
    23521 atggcggctt cgcacccggt ccacagggat atcgtgctcc agtgcccact gccgcagcag
    23581 attgtcactg ccctgactgt caaccagata aatatccacc tgccgccccg aagacagcac
    23641 tgatgccagt cgtgcatcac aggtgccaca gtctttcgct ttcacaaaca acgccagccg
    23701 tccgccgttg tcgttcagca gcccgccccc tttccccagg ctgaccggca taacgcccgg
    23761 ataaaggcgc ttccaggccg cattgacttc ccgctgaaat gccagttcct tctcagtccg
    23821 ctcatattcg gccttcaccc atttctcagc cagctgccgg cgttccgcat ccgtccgtgc
    23881 ttcaatcccc agcaccgtca gcggatccag accaggggac tgcatccccc gcggtccctc
    23941 catcagagcc cggtatcgac ggtaatcctc ctccgtgaca ccccactgag cggcctgttg
    24001 ctgcagcgcc tgtgtggtgg tactgacttg cgaagaggcc tgcgtgcgtg tgccctcggg
    24061 cgcactctcc cgtacagcag ccaccgtctg cagggaaatg agcagcatga gcaccggtga
    24121 cagattcttc attttcattt catcactcca ccatgacccg gacctgacgt ccgttcaccc
    24181 ggaacgaggc ttctccccgt ccggcacgct caagcatcca gccggccgtg ctctcccctt
    24241 cacccagcag cgtcacatct gaaagacggg taaatccacg gggagccacc gcagcatatg
    24301 tgcgtccgcc acgctgctcc gtgcctgtca gcacaaacgg tgcgctgcgg ctgataactg
    24361 aaatgccgga agagcccttt ttctggcgtg gcgtgaccgc cggtgtgctc ttcaccgtgc
    24421 ccgttccttt cgcagatgac ggtgataccg gggtgaatgg cgccttctcc agcttctcca
    24481 gtcgcaggtc atacgcacga agcgtttctc cctgctgcgc cagcgtggcc tgcagtgatg
    24541 ccaggctggc ggccatctga ccagtgcgtg catgctcttc ctccgtcagt acaggtcgtt
    24601 gctccagcaa tgtcagtcgc tccgtcacgc ttttcagctg ttttttggtt tcagccagcc
    24661 gggacctgag cggttcaagc tcacttcccg acaacgcgag agcatctgaa tacgttttca
    24721 tttgcccctc cagtgcggca atgcgttcat cctgctcccc gctgtaacga tagaggccgc
    24781 aaaccgccag aatggtcgca accagaacgg tcagtacaaa tcccacggaa agcatgggtg
    24841 acacacaggt ccacccatca agaggagatg aatgcactga cggtaatgtc ttctgttttt
    24901 tttcactcat tttttaagct ccgctcctgc gcccctgaca ccggccggta ccggcgccag
    24961 ctggcccggc ggcaactgat aaccttcccg caggctgtga cagacaaccc gctgcacatc
    25021 atcaacttca agttgccacg ccggaccggc catcatctgc agggcggtac gcaacctgag
    25081 cggccccgtc tgatactgca ctgccggcaa cgcctgacga taaagcaccc cgttggccgg
    25141 tccggtggca cagagcgtgt agcccgactg tttcagggca taacgcatcg catctgccac
    25201 cgtcggcttc agggatgccg gaatacggat gtcaatcacc tgggaaaggg gatcccgctg
    25261 taccgcatcc ggatgagtgt taaccagcag atagcggtca taacgcacca cctcgggcac
    25321 agtcgtcccc gcgtacatat cagggctggc cggacggaca ttattgctga ccgtcaccga
    25381 cggtaccgga acagccgcat cccgctgcag cagtttttgc tgcccctgac agccagtcag
    25441 aagtacgcag gcggacagca cggaaagaag aagggatgtt ttcatcagga agttacctct
    25501 gcggtgaagt gataaagtca gaggtcaatt tgcagaagaa gtccagaccg gctcaatgaa
    25561 caactgattt ccggttgaca aaaaaaacgc ctgcacaagg caggcgtggt gtaaaaggga
    25621 acagagtctt ccggttattc agtcctgatt tagcgcacca ttacgctgca ccgggacgta
    25681 atccagagcc tgcagcaact gctcatctgt cagatccaga aaatctttga tttcctccag
    25741 agattttccc ttttgtctca tcatgagcac taatctggag cgctcttcag cacgaccttt
    25801 ctccagacct ttctcttcca gttgctctgc tatcgtcatg atcaccccct tgtggctggg
    25861 atagtgtgtg gatactgctt cagaaagttc agcaaaatcc atctggtaaa gttccatcag
    25921 ataagtcagc acgatattct ggtgaacgca actgccatac ttatccgtca gtgtgatgga
    25981 tttaccagtc agctcaatat ctgtcactgc cagaaactac caaaaaaacg cctgcacaag
    26041 gcaggcgtgg tgtaaaaagg gaacagcgta ttccggttat tcagtcctgg ttaagcgtac
    26101 cattacgctg caccgggacg taatccagag cctgcagcaa ctgctcatct gtcagatcca
    26161 gaaaatcttt gatttcctcc agagattttc ccttttgtct catcatgagc actaatctgg
    26221 agcgctcttc agcacgacct ttctcttcca gttgctctgc tatcgtcata atcaccccct
    26281 tgtggatggg atagtgtgtg gatactgctt cagaaagttc agcaaaatcc atctggtaaa
    26341 gttccatcag ataagtcagc acgatattct ggtgaacgct gctgccatac ttatccgtca
    26401 gtgtgattga tttaccaatc agctcaatca catcaccact ggtgccggac ttcatcgtga
    26461 acatcagcgc cgccatccgg tgatagtgtt caatttcatc atcggtaagc gtgctgacat
    26521 ccaccaagct gaatggcttt tgctcgccat aaatctcgcg tccgtacacc gggtcatcca
    26581 gacaatccac ccagttcatc ggatacggat atggccgctt tgcaccatga taaaacagca
    26641 ccgggatcac tactggcgca cacttatgct cttcaatatg ctgctccatc accgccagaa
    26701 tataacgcat catgcgcagt gggataaatt tatccggcga actctggtgt tctattaaag
    26761 tataaatgta cccgtccttc ccgtcatttg tcttcatgct gagcaaaaca tcgcaggcag
    26821 actgacgtaa actttctgcc acaaacgtcg tggattcaag ttctattgta tcaagattac
    26881 agcgggattt ctgatacggt gtcaagacct gttcaagaaa gtccctcgcg gctgcagggt
    26941 ccttcagtgt cttcctgaac gccgcatcat gtatcaggct ggttttatta actttttcac
    27001 tcacggaacc tccggataaa aaagtggggc ataacgcccc atggttgatg ttacatggca
    27061 tgaataattc gtcgttccat gccatacccg agccccataa agggaatatc atcctcaaag
    27121 tccattgggg gtgcaccatt acccgttgat gttgtctggg gtgcacgacc agcctgtccg
    27181 gccgtcttcg gcggctgtcc ccagccccct tgtgcaggtg cagaagcacc attaccggca
    27241 gtgccctgct gacgcagtga acccagcatc tgcatgacac cattcactcc cacaacaacc
    27301 tcagttgaat aacggtccac accgttctgc tggtcaaacc acttacgggt tttcagctgg
    27361 ccttcgatat agaccttaga gcccttacga agatact
//
LOCUS       DAZGJT010000061        27387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZGJT010000061 DAZGJT010000000
VERSION     DAZGJT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.061"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..397
                     /locus_tag="ACHKFK_002848"
     CDS             <1..397
                     /locus_tag="ACHKFK_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFO9053141.1"
                     /translation="LDSHHHVHMFPQIFPIVAKFAAEEGIALRIDRQPLSNDGDLPAN
                     LRSSQGFSSAFYGEEISEALFLQVLDDSSHRGERSLEVMCHPAFVDNTIRQSAYCFPR
                     LTELHVLTSASLKYAIAERGYRLGSYHDV"
     gene            complement(444..2705)
                     /gene="katE"
                     /locus_tag="ACHKFK_002849"
     CDS             complement(444..2705)
                     /gene="katE"
                     /locus_tag="ACHKFK_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO9053142.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDTNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQVAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQHHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIANNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIAEADSADGSFMDELLTLMTAHR
                     VWSRIPKIDKIPA"
     gene            2888..3151
                     /gene="cedA"
                     /locus_tag="ACHKFK_002850"
     CDS             2888..3151
                     /gene="cedA"
                     /locus_tag="ACHKFK_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO9053143.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQENEVEE"
     gene            complement(3198..4589)
                     /gene="tcyP"
                     /locus_tag="ACHKFK_002851"
     CDS             complement(3198..4589)
                     /gene="tcyP"
                     /locus_tag="ACHKFK_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO9053144.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGLLLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSKDDAELAHR"
     gene            complement(4722..5312)
                     /gene="ydjM"
                     /locus_tag="ACHKFK_002852"
     CDS             complement(4722..5312)
                     /gene="ydjM"
                     /locus_tag="ACHKFK_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310461.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9053145.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPESWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVSQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(5476..6144)
                     /gene="hxpB"
                     /locus_tag="ACHKFK_002853"
     CDS             complement(5476..6144)
                     /gene="hxpB"
                     /locus_tag="ACHKFK_002853"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO9053146.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVEQVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFNLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6291..6827
                     /gene="yniB"
                     /locus_tag="ACHKFK_002854"
     CDS             6291..6827
                     /gene="yniB"
                     /locus_tag="ACHKFK_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO9053147.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNVFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PLICIAAGYVFFSLLGFI"
     gene            complement(6868..7728)
                     /gene="yniA"
                     /locus_tag="ACHKFK_002855"
     CDS             complement(6868..7728)
                     /gene="yniA"
                     /locus_tag="ACHKFK_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO9053148.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGRDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(7834..8124)
                     /gene="ghoS"
                     /locus_tag="ACHKFK_002856"
     CDS             complement(7834..8124)
                     /gene="ghoS"
                     /locus_tag="ACHKFK_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9053149.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDDG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(8225..9154)
                     /gene="pfkB"
                     /locus_tag="ACHKFK_002857"
     CDS             complement(8225..9154)
                     /gene="pfkB"
                     /locus_tag="ACHKFK_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO9053150.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHGDTQKIYAYLSR"
     gene            9440..10198
                     /gene="ydiY"
                     /locus_tag="ACHKFK_002858"
     CDS             9440..10198
                     /gene="ydiY"
                     /locus_tag="ACHKFK_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO9053151.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(10251..10358)
                     /gene="yniD"
                     /locus_tag="ACHKFK_002859"
     CDS             complement(10251..10358)
                     /gene="yniD"
                     /locus_tag="ACHKFK_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO9053152.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(10529..11558)
                     /locus_tag="ACHKFK_002860"
                     /pseudo
     CDS             complement(10529..11558)
                     /locus_tag="ACHKFK_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            12081..14009
                     /gene="thrS"
                     /locus_tag="ACHKFK_002861"
     CDS             12081..14009
                     /gene="thrS"
                     /locus_tag="ACHKFK_002861"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO9053153.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSDYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            14013..14555
                     /gene="infC"
                     /locus_tag="ACHKFK_002862"
     CDS             14013..14555
                     /gene="infC"
                     /locus_tag="ACHKFK_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO9053154.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            14652..14849
                     /gene="rpmI"
                     /locus_tag="ACHKFK_002863"
     CDS             14652..14849
                     /gene="rpmI"
                     /locus_tag="ACHKFK_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO9053155.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            14902..15258
                     /gene="rplT"
                     /locus_tag="ACHKFK_002864"
     CDS             14902..15258
                     /gene="rplT"
                     /locus_tag="ACHKFK_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO9053156.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    15375..15499
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            15381..15425
                     /gene="pheM"
                     /locus_tag="ACHKFK_002865"
     CDS             15381..15425
                     /gene="pheM"
                     /locus_tag="ACHKFK_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO9053157.1"
                     /translation="MNAAIFRFFFYFST"
     gene            15709..16692
                     /gene="pheS"
                     /locus_tag="ACHKFK_002866"
     CDS             15709..16692
                     /gene="pheS"
                     /locus_tag="ACHKFK_002866"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO9053158.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            16707..19094
                     /gene="pheT"
                     /locus_tag="ACHKFK_002867"
     CDS             16707..19094
                     /gene="pheT"
                     /locus_tag="ACHKFK_002867"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO9053159.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHNGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPIAVEAPDACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLDADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            19099..19398
                     /gene="ihfA"
                     /locus_tag="ACHKFK_002868"
     CDS             19099..19398
                     /gene="ihfA"
                     /locus_tag="ACHKFK_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO9053160.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            19499..20479
                     /gene="btuC"
                     /locus_tag="ACHKFK_002869"
     CDS             19499..20479
                     /gene="btuC"
                     /locus_tag="ACHKFK_002869"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO9053161.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGELWILPGDWF
                     SPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            20541..21092
                     /gene="btuE"
                     /locus_tag="ACHKFK_002870"
     CDS             20541..21092
                     /gene="btuE"
                     /locus_tag="ACHKFK_002870"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO9053162.1"
                     /translation="MQDSILTTVVKDIDGKVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            21092..21841
                     /gene="btuD"
                     /locus_tag="ACHKFK_002871"
     CDS             21092..21841
                     /gene="btuD"
                     /locus_tag="ACHKFK_002871"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO9053163.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPANLAQAYGMNFRRLDIEGHRMLISTI"
     gene            21919..22383
                     /gene="nlpC"
                     /locus_tag="ACHKFK_002872"
     CDS             21919..22383
                     /gene="nlpC"
                     /locus_tag="ACHKFK_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO9053164.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            22630..23343
                     /gene="ydiV"
                     /locus_tag="ACHKFK_002873"
     CDS             22630..23343
                     /gene="ydiV"
                     /locus_tag="ACHKFK_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO9053165.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQDLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNFAG
                     ELLKYPFIELLINENYPHFNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQIIPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            23406..24842
                     /gene="selO"
                     /locus_tag="ACHKFK_002874"
     CDS             23406..24842
                     /gene="selO"
                     /locus_tag="ACHKFK_002874"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO9053166.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVESGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLADEEDKYRLW
                     FTDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDGLNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEITDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(24846..25037)
                     /gene="hemP"
                     /locus_tag="ACHKFK_002875"
     CDS             complement(24846..25037)
                     /gene="hemP"
                     /locus_tag="ACHKFK_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO9053167.1"
                     /translation="MRYTDSRKLTPETDASHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(25169..26215)
                     /gene="aroH"
                     /locus_tag="ACHKFK_002876"
     CDS             complement(25169..26215)
                     /gene="aroH"
                     /locus_tag="ACHKFK_002876"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO9053168.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQPRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHAEDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(26372..27205)
                     /gene="ppsR"
                     /locus_tag="ACHKFK_002877"
     CDS             complement(26372..27205)
                     /gene="ppsR"
                     /locus_tag="ACHKFK_002877"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO9053169.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         6913 a         6458 c         7069 g         6947 t
ORIGIN      
        1 tcttgatagc catcatcatg tgcatatgtt cccgcagatt ttcccgattg tggcaaagtt
       61 tgcggctgaa gaggggattg cgttgcgcat cgaccgtcaa ccgctatcta acgatggtga
      121 tttaccggct aatctgcgca gttcgcaggg attcagtagc gcgttttatg gtgaagagat
      181 tagcgaggca ttgttcctgc aagtgcttga tgattccagc catcgtgggg aacgctcgtt
      241 ggaagttatg tgccatccgg cgtttgtcga taatacgatt cgccagagtg cttattgctt
      301 ccctcggtta acggaactgc atgtgctgac ttcagcgtcg ttgaaatatg cgattgctga
      361 gcgtggttat cggttgggta gttatcatga tgtgtaagta aggtggtaag gtcactcccc
      421 aggaggcgtg accttatcca tatttacgcc gggattttgt caatcttagg aatgcgtgac
      481 cacacgcggt gtgctgtcat cagcgttaac agttcatcca taaaactacc atcggcgctg
      541 tcagcttccg caatcccttc ttcaccctgg tcagcgacct tgattgttgc tttaaacttg
      601 cgcgcgtctc ctgccagcgc aatcggttta aggtgtttgt aggcttccat cagatagtag
      661 ttggcatcgc cgttgttagc gatatccgca atattgccgc aagggacaat gactgcatcg
      721 accgtcagcg aaggcgcacc ggcaaaggta gcagctatag gcaacaccgt accgtcatcc
      781 gcagtcactt cccccattcg ggagtagagc agtttggcat gaacgccttt ggccttcagt
      841 gccttgagaa tggccagaag gtctgccgat ctcacttcat cattaagcaa aattgctacc
      901 acgcgacctt tcacatcacc gtcaggaatg gcgtacagac ttaaggatgg atcctttttc
      961 agaccgttga cgtccggagg cggggtgata ttcaactgat cgtcagtcag ttcgataccg
     1021 agatttttcg ccaccgcctg ggccagagtg agatcaatat gcgccagctg gtcaacaacg
     1081 cgctcacgaa tatacggacg aactactttg cttaactcaa aactgaaacc atcgacaata
     1141 tggtgctgct cgaatggcgt ctgacttagc cagaacagac gcggatggga ataatattcg
     1201 ccaaacgatg gactgcgctc gcgaacttta ttcccttcca cgcgttcctg gtatgattca
     1261 aaaccgccgc gtttcggccc cggcggtgtt tcgcgcggcc agttatcgtt gatcgagttc
     1321 ggttcgtaat tcgccgggtt agtgtcgatc cccatacgat gcatgccgtc acgctggaaa
     1381 ttatggtaag ggcaggtcgg gcggttaatc ggaatttcat ggaaattcgg cccaccaaga
     1441 cgactgattt gtgtatcggt ataagagaac aaacgcccct gcaataacgg gtcgttggtg
     1501 aaatccagac cggggacaat atgccctgga tggaaagcca cctgttcgtt ttcagcaaag
     1561 aagttatccg gattacgatt gagcaccatt ttgccgacac gctgaacagg caccaattct
     1621 tccgggataa gtttggttgg atcgagaaga tcgaagtcga acttgaattc gtcttcttca
     1681 ggaatcaact ggaagcccag ttcgtattca ggaaaatcgc ctgcttcaat cgcttcccac
     1741 aactcgcggc ggtggaaatc agggtcacgt ccggttaatt tttgtgcttc atcccaaacg
     1801 agtgaggctt tacctgccag tggtttccag tggaaacgta cgaacgttgc cttcccttcg
     1861 gcattaatca ggcggaaggt atgaataccg aagccttcca tggtgcggta actgcgcggg
     1921 ataccgcgat ccgacatcgc ccacatcacg ttgtgcagag tttcaggttg cagagaaaca
     1981 taatcccaga aagtatcgtg ggcgctttgc ccttgtggaa ttgcccagtg cggttctggt
     2041 tttaccgcat gaacaaaatc ggggaattta tgcgcatcct ggataaagaa gattggcgtg
     2101 ttattgccaa cgaggtcaaa aataccctct tcagtataga acttggtggc aaagccacgg
     2161 atatcacgca cggtatcggc agagccagcg ccgccctgaa cggtagagaa cctaacaaat
     2221 actggggtga ttttgttcgt atctgagagg aaatctgctt tggtgatatc gcttaagctt
     2281 ttatatggct ggaaataacc gtgagctgct gatccgcgtg catgaacaat acgttccggg
     2341 atgcgctcat ggtcaaagtg agtgattttc tcgcgcagaa taaaatcttc cagcagagtt
     2401 ggaccacggc taccggcacg tagtgagttt tgatcgtcgg cgatgcgcac gccctgatta
     2461 gtggtcagcg cataattttc actgcctttg cgtacgtctt ccagagaatt aagtttttcg
     2521 ttacgcgtat caggggcttt caggctccct ggggcggtag gttgtgcacc aggcggtgtt
     2581 ggttcagccg ctggacgatg agatccgtct tcaggtgcca gtgagtccat ccccggtttc
     2641 gcttcgctgg aatcgtgtag tggtgactgg tgctgatgtg ggttcttttc gttatgttgc
     2701 gacattgaac tcgtctcctt aaattattac tgaaagggcc gctgttttgg tcaaaaaacc
     2761 ctctaactat agaccaccag ccagatcccg cttcggaaac tattctggaa acggtgttat
     2821 gaaatacacg caaagcggga cgtacagggg ctaaatcggc taaactacag tttttctgat
     2881 ttttaatatg cgtttagtga agccagtaat gaagaaaccg ctccgtcagc aaaaccgcca
     2941 gattattagc tatgtcccac gcacggaacc cgcgccgcca gaacatgcga taaagatgga
     3001 ttcgtttcgt gatgtctgga tgctgcgtgg caaatatgtt gcgtttgtac tgatggggga
     3061 gtcatttctg cgctcaccgg cgtttactgt gcctgaatcc gcccagcgtt gggcaaatca
     3121 gattcgtcag gaaaatgagg tcgaagaata aaaaaacacc tgtcgggatg acaggcgttt
     3181 aacgtaacaa atcaggatta acggtgtgcc agttcggcgt cgtctttgct atccagaatg
     3241 gctttatcgg tttgcttcag ccactggctg gtcagcgtgc cagcggtcat cgagccacta
     3301 acgtttagcg ccgtacgacc catgtcgata agcggttcaa cggagattaa caatgccacc
     3361 agcgttaccg gcaggcccat cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca
     3421 ccgacaccgg caacgcctgc ggaactaacg gtaacaatac cgaccagcgt cgcaatccac
     3481 atcgggtcca gcgggttaat gcccaccgta ggcgcaacca tcaccgccag cattgccgga
     3541 tacaaaccgg cgcagccgtt ctgaccaatg gtcgcaccga aagaggcggc gaaactggcg
     3601 atggattcag gaacacccag acgacgcgtt tgtgcttcca cattcagtgg gatagacgca
     3661 gcactggaac ggctggtaaa ggcaaacgtc agtactggcc ataccttacg gaagtacttc
     3721 agcgggctca cgccattaat gcccagcaga aggccatgca ctgcaaacat aatcagcaga
     3781 ccgaggtagg acgcaacaac gaaacttccc agtttgatga tgtcttgcag gttagaacct
     3841 gcaaccactt tggtcatcag cgccagaaca ccgtaagggg tcaactgcat gaccaggcga
     3901 accagtttca tcacccagct ttgcagggta tcgatagcgg ttaagacgcg ttcacctttc
     3961 ggcgcatcat ccttcagcag tttcagcgca gctacgccga ggaatgcggc aaaaattacc
     4021 acgctgataa ttgacgtcgg attggctccg gtcagatcgg caaacgggtt tttcgggata
     4081 aaggacaaga ccagctgcgg aacgctcagg tcagagactt taccaacata gttactttcg
     4141 atggcgttca gacgtgcagt ttctgcacca ccctgaacca gaccttcagc cgtcaaacca
     4201 aacaggttgg tgaccagcac accgaccagc gctgcaatca gcgtggtaaa caaaagcgta
     4261 ccgatggtca gaaaactgat tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg
     4321 ctcagaatag aggcgaagac tagcggcata acgatcattt gcagcagttg aacatagccg
     4381 ttaccaacaa tgttaaacca ctgtacagaa tctttaagta cctggctgtc agaaccataa
     4441 atggtatgca gggcaaggcc aaaaaccaca cccatcacaa gacccaccag cacttttttc
     4501 gccagactcc actgtttgtg gcgggtttga gccagcgcaa acagcagtac aacgaacacc
     4561 acgatgttcg caattaatgg aaagttcatc cccgttctcc tgatcttatt atttctatca
     4621 ctgcgtaaca gtcatagtgt tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt
     4681 caaatggaat tgcttattcc agaaaggcga tttttgcctt tttagtattc aacctgatgc
     4741 ttaataagcc gatgaaactg gatctgtaag gtattgatca tttgcgatga ccaacgaaca
     4801 gcgctgtttt cgggtaatga atggggcatc cataccgacc agacaaataa tgccatgcag
     4861 ataaaacgtt ccagttggtt gcccttttgg gaaaccagga taggcaagcg gaaacgccag
     4921 cggcatggcc agagcagggg aacaccggcg ggtgtcagca tatcggcaag tatgtggctc
     4981 aaataaccca gcaccatacc ctgcaacgca tcagccggaa tgaaccagct ttcaggaact
     5041 ttaaggtaaa aggttgccag cagcgcaaat accgccagca gactgtgggt aaaaccacga
     5101 tgcccaaaag cgcgggcgat cggttttgat atccacttta atcgctgccc aagaaacgac
     5161 tttgggtgat cgatgtccgg taacaaacac gtcaggattg cggaagggac aatatgccac
     5221 cagtcaccct gtgccagcac gggcgtcagc tcggcatttt tggcaaatac cgcacaagca
     5281 atagaaaaga gaaggtgacc ttccgccgtc atgataaaac tcactaaact gtcgatacgt
     5341 acagtatagg gtttttatac agtgcacgga agtggtgacg gtgtaactct ttctcttaaa
     5401 ttggcgatta atccgtcagt tgaatgcaaa ttcagataaa gaaccagggg cagtgcgatc
     5461 actgccccct ggtcattaac cgagaaggtc ttttgcggtg agttctgtca gcgatgagag
     5521 tttgacgttt gctaatacaa aacgtggatc attttgcgct tctggcgcag gaacgacgat
     5581 ggaacgcatg cgggctgctt tagaggcgat catgccattt accgaatctt ccagtgccac
     5641 gcaggtcaga gggtcaaccc ccagttttgc tgcgcagtcg agatatactt gcggatgcgg
     5701 cttgctgtaa ggcagttttt cagccgaggc gagggcatcg aaactgtcgc gtaagttaaa
     5761 catggtcaac actttttcca gcatatgtag tggtgacgcg gaggccagtc ccaccaataa
     5821 accttgttct ttgcataacg caacggcttc acgcacgcct ggcaataatg ggcgtgtctc
     5881 ttcaaccagt gaaatggcac gggcaataac ttgttctact acttcctgac ggcttggccc
     5941 attccatggt tgccgggcgt accaaagatc gaccaccata tcgatgcgta aacctaaggt
     6001 gtccggcagc tcgttacgac gggagatatc cacccccagg cttgccatca catccagttc
     6061 ggctcgatcc cataaaggtt ctgagtcgat aagtaatcca tccatatcaa aaattgcagc
     6121 aagaatctga cgcggggttg acattacaac ctctccttta gagcattata tgtaaggtat
     6181 taagccgtgc ttatgattaa gcgcctatca tatcgccttt aaagaatggg cgaaaacaga
     6241 tatctacagg taaactaagt gaaacgacgc gtcttaatca aggagaactc atgacgtatc
     6301 aacaagctgg acgcattgct gttttgaaaa ggattttggg ttgggtgatt tttatacctg
     6361 cattgatctc tacattgatt tctttgctga aatttatgaa tacccggcag gaaaaccagg
     6421 aaggcattaa tgcggtcatg ctcgacttta ctcatgtcat gatcgatatg atgcaggcga
     6481 atacgccttt tttgaatgtg ttctggtaca actctccgac gcccaatttt aatggtggcg
     6541 tgaacgttat gttctgggtg attttcatcc tgatttttgt cggactggcg ttgcaggatt
     6601 ccggagcccg gatgagtcgc caggcgcgct tcttgcggga aggtgttgaa gatcaactca
     6661 ttctggaaaa agccaaaggg gaagaggggc taacgcgtga acaaattgag tcacgtattg
     6721 ttgttccgca ccatactatt ttcctgcagt ttttctcgct gtatatcctg ccgcttattt
     6781 gtattgctgc aggttatgtg ttcttttctc tgcttgggtt tatttaatca aaaggccgca
     6841 cggagcggcc attatcctca acccatatca tgctgctaat aatctatcca gtgactgctg
     6901 agcaataacc aaatgctgac cgccaaataa ccttgcacga tttaacagcg tgtagagttg
     6961 gtaaaccggt tgacgttcaa ggaaatcggc aggtagcggg gatactgact gatagccgtc
     7021 atagatttgt ggcggctgtt cagtatgcag cggtaacatc gccaggtcgc actctcggtc
     7081 accccagtaa caggccgggt cgaaaatgta cgggccatcc ggacccagcg cacagttgcc
     7141 ggaccataaa tcgccgtgca acagagaagg ctgcggttga tgcgaggcga gacgctgctg
     7201 aatatgctcg acgatggcat cgatattgcc gaaagcgatc cctttttctg ccgccagttc
     7261 cagctgccag ccaatccgtt gttcagcaaa aaaagttgac cagcgacgtt gccaggtgtt
     7321 gggctgtgga gttgtggaga gcgcgttatc gaaatcgagg ccaaattgtg gttggtcgct
     7381 ccattgatgt aaacgcgcaa tttgctgacc aagaataaat gcactatgcg catccagcgg
     7441 gcgaggtggg agataatcca tcaccagaaa actgtagtca cggtcagcgc caactgccca
     7501 aaccttaggc acggtgacgg ttttactacg cgacagtaac tccagttggt cggcttcggc
     7561 ggtaaaaccg ggaagcagtt ccctttcatc acatttgacg aaaaagtcac gtcctgcata
     7621 gcgcaaatgc catgcggcat ggacttctcc gccaggcagt tcattacgca gttcgatttc
     7681 gccttcacct aactgctcgc tcaaaagacg actaattgcc tgccacatga tgtctctccc
     7741 atgttgtctg ccagctcata aggttagcgc aagaatgcgg tgtaaaaaca cgagctaacg
     7801 cacaccacag actgattatt gcccaaaaac catttatttt ctgttgctat tccattcctc
     7861 ccaggtcgtg atggtgattt caggatcttt gccggttgca ctttgggtaa gccccgaaac
     7921 tagttctcta atttcttctt cactttgggt actgataagt ccaaaagtgt tagtccccag
     7981 ctcatggata ttgccatcat catccgtcat ggtgagcaaa aaaccgtcgc gagtcaggta
     8041 attattcaac tcgttaattt cagtcaatgt atcctcatgc aacatgacgg ttattacata
     8101 acggacaaga tcgccactgg ccatagttca cccctttgtt atcacatgac ttttgagcat
     8161 agtcggagaa acgcgttgcc gacaggttgg tggtgattcc cccaatgctg ggggaatgtt
     8221 tttgttagcg ggaaaggtaa gcgtaaattt tttgcgtatc gccatgggag cacagacgtg
     8281 ttccctgatt gagtgtggct gcactccccg cagctacgcc aaaacggacc atttcttcaa
     8341 gagaggcatt ttctgccagt ttcagtgtca tcgcgccgac catgctgtca ccagcgccaa
     8401 cggtactctg gcttttcacc ggtggtggca cgacctgaat gcagttttca ctatcaacgc
     8461 ccagcgcccc ttgtggaccc agggaaacga caacccgttt ggccttgccg ctattaacga
     8521 tttcctgcgc ggctttgcgg acatcatctg gctgggtgag ttcgcgattt accagcgcac
     8581 tgagttcttt ttggttaggc ttaaccaact cgatgttacc aatcgccagt gctgcactta
     8641 acgcttcgcc agaactgtcg acgatgcagc ggatcccttg tttttgcgca gcggaaatca
     8701 gttgggttaa tttttccagc ttcacacctg gcggcaggct tccgcttatg accagaatgg
     8761 ccccggattc aatttccaga acttgctctt caagctggcg aaactcatct tcatttaatg
     8821 ccgcgcctgg cataacaaaa cgatactgct caccgcttgc ttccacatgt acgtgtaaat
     8881 tctgccgggt ccagtctttg gcttctacag tagcgacggg gacattttca tctgccaaca
     8941 gtgaaaccag atgttcgccg gtcgcaccac ccgccgggaa gatcgctgtg gcactgcctc
     9001 caagatgggc aatggcgcgg gcgacgttga tgccgccgcc tccgggttcg aataccggtg
     9061 cggtacagcg cagttttcct tcgggataaa tttgcggggt aattgttgcg ctatcgagtg
     9121 agggcgcaag tgtcaacgta tagatacgta ccatcatttc ctcctatagg ctgatttcag
     9181 tctggcaccg aatcagcgga aagtgaagaa attaacaata tgatttattg gagctttact
     9241 taaaattttg cagataaata tatataaata aaaatctgtt ttcgccacat gcttattcaa
     9301 aaaatgaaat aagaatacat tgctaagtta ttcgcgcctt tttataattc gttacctttc
     9361 cagggacgct aatctttatc cttaagaaag tttccgggac cgcgatggtc tctttttttg
     9421 ttgtacggac tcttgataaa tgaagctttt gaagacagtt cctgccatcg ttatgttggc
     9481 ggggggcatg tttgcgtcac tgaatgcagc tgccgatgat tccgttttta ctgtcatgga
     9541 cgatcctgcc agcgctaaaa aacctttcga aggtaatctg aacgcaggat atcttgcaca
     9601 atcaggcaat acgaaaagct cctcacttac ggctgacacc accatgacct ggtatggaca
     9661 aaccactgcc tggtcgctgt gggggaacgc cagtaatact tcttctaatg atgagcgttc
     9721 ttcagaaaaa tatgcggcgg gcgggcgtag ccgttttaat ttaactgatt atgactattt
     9781 atttgggcag gcaagctggc tgacagaccg ttataacggc tatcgagagc gcgatgtgtt
     9841 gaccgcgggt tatggtcgcc agtttttgaa tggcccagta cacagtttcc gctttgaatt
     9901 cggcccaggt gtgcgttacg acaaatatac cgacaatgcc agtgaaaccc agccgctggg
     9961 ctatgcctcc ggggcttatg catggcagtt gactgacaac gctaaattta cgcagggtgt
    10021 ttcggtcttc ggcgcggaag acacaacgct gaactccgaa agcgcattaa atgttgctat
    10081 taatgaacat tttggactga aggtggctta caacgtcaca tggaactctg aacctccaga
    10141 gtctgcaccg gaacataccg atcgtcggac aaccttatcg ctgggctaca gcatgtaaaa
    10201 ttatctgccg gaccgtgtta tgcggtctgg ctgttttcgt ttgtcactgc tcagccccaa
    10261 atcattgctg cccaacgtag caacaacata gcgccacaga ttgccagtag caccaccacc
    10321 atcttccagt gttttttatc aaagcgttta gtcggcatac cagtgtcctg catatctctt
    10381 gtgcgtgata acggagtgta acaaaaggga caggcatcaa gggatttttt gttattacag
    10441 tcgtcgggtg gcgttgtaca cctggtcact gtttctgcat tttatgaaaa aaattatgta
    10501 tcgatataca aatatcataa atagcgggtt ataaatacgt ctttagttca tcagcactaa
    10561 aactcaggct ttggctgatt acatttatag cagcataata tgtttctaca gttttatgat
    10621 gattatgatg aatggctata tgaacagctg acatgttgtc atgattttta gcggccaaca
    10681 gtgtaaataa ctgacgttgg ctaaaagaat atttttttca aaagtaccca acgttgatat
    10741 ataagataac acgacatctt tacttactga caaagtatga ttgtctatat ataaaatcgc
    10801 ggacatatga ctgacattgt ttgccatcat tcgacagaag aaaatatcta aagcaaagcc
    10861 aaaatcctga ctcgcgataa cgcgactcct tgcaggagga tggcgaagta atgctctgaa
    10921 ctccgcgtcc cggactaagg agacatgctg ctgtatggat tcaacagagg agtaatgttt
    10981 aatcaggttg actttgcctt ttccatttct caatgaatca agggcgtatt gtaatgacgg
    11041 atggcgacaa aaaatagtgt cagaaggaga ttgcaaaaaa atgcactatc attgagattt
    11101 actgttggct gtatggtttt ttctctatga atttttttgg acagtttcat gcaatggtta
    11161 cgtttttcgg cagttaactg ttcctgttca atataatcca gtatgcttcg caaatggtta
    11221 gctttaatag gcgtgatttc cagaaataag gaaatgattt cttttttata gtgtaatgta
    11281 atgagtgtat tttctgcatg gatgggtgaa gtggcaaagt taatgataaa aagggcagtg
    11341 gaataacaat tttgtgaaat ttttcgacag attgcggtaa attcctgagt aacacagtat
    11401 gacagcatcg aacttaaata agaggtatca agtagggtga tgagctggcg cgcaaattga
    11461 ttaataggct ctgatgaagg cgttagtatg tcgctatcga tgtgcattct aagaggagac
    11521 tcataatgag ttctgataat gatatcgttt tcactcataa tctattcgcc ttatatatgt
    11581 ctggagattt tgaggatgat gattaattat tatcgccaga acgaaatgcg cacgaaaaat
    11641 tgctataaga attgtgtcac ttttatcatt gtgatatgac tacttcgtca ttaatctcat
    11701 gatcgtttaa atactaccgc caaatcacgc aacgaacttt gctggattca gtttatgctg
    11761 ctgtaaatcc gctcgtagta aacctttcag acactctgtg atattatcag tggttcttta
    11821 agcgttttgc tggtgtactc actacaaacg aattgcgaat cgaatcaatg tgaaacggaa
    11881 aggtacaatc tccctttttt tagtgttaac atcgttcaac cgggtggaag gtgagaggtt
    11941 aatgtctttc atttgtgtac ctgattgcga accaatttag catttgttgg ctagatggtt
    12001 tcgcaatgaa ctgttaataa acaaattttt ctttgtatgt gatctttcgt gtgggtcacc
    12061 actgcaaata aggatataaa atgcctgtta taactcttcc tgatggcagc caacgccatt
    12121 acgaccacgc tgtaagcccc atggatgttg cgctggacat tggtccaggt ctggcgaaag
    12181 cctgtattgc agggcgcgtt aatggcgaac tggttgatgc ttgcgatctg attgaaaacg
    12241 acgcacaact gtcgatcatt actgccaaag acgaagaagg tctggagatc attcgtcact
    12301 cctgtgcgca cctgttaggg cacgcgatta aacaactttg gccgcatacc aaaatggcaa
    12361 tcggcccggt tattgacaac ggtttttatt acgacgttga tcttgaccgc acgttaaccc
    12421 aggaagatgt cgaagcactc gagaagcgga tgcatgagct tgctgagaaa aactacgacg
    12481 tcattaagaa gaaagtcagc tggcatgaag cgcgtgaaac tttcgccaac cgtggggaga
    12541 gctacaaagt ctccattctt gacgaaaaca tcgcccatga tgacaagcca ggtctgtact
    12601 tccatgaaga atatgtcgat atgtgccgcg gtccgcacgt accgaacatg cgtttctgcc
    12661 atcatttcaa actaatgaaa acggcagggg cttactggcg tggcgacagc aataacaaaa
    12721 tgttgcaacg tatttacggt acggcgtggg cagacaaaaa agcgcttaac gcttacctgc
    12781 aacgcctgga agaagccgcg aaacgcgacc accgtaaaat cggtaaacag ctcgacctgt
    12841 accatatgca ggaagaagcg ccgggtatgg tattctggca caacgacggc tggaccatct
    12901 tccgtgaact ggaagtgttt gttcgttcta aactgaaaga gtaccagtat caggaagtta
    12961 aaggtccgtt catgatggac cgtgtcctgt gggaaaaaac cggtcactgg gacaactaca
    13021 aagatgcaat gttcaccacg tcttctgaga accgtgaata ctgcattaag ccgatgaact
    13081 gcccggggca cgtacaaatt ttcaaccagg ggctgaagtc ttatcgcgat ctgccgctgc
    13141 gtatggccga gtttggtagc tgccaccgta acgagccgtc aggttcgctg catggcctga
    13201 tgcgcgtgcg tggttttacc caggatgacg cgcatatctt ctgtactgaa gaacaaattc
    13261 gcgatgaagt taacggatgt atccgtttag tctatgatat gtacagcact tttggcttcg
    13321 agaagatcgt cgtcaaactc tccactcgtc ctgaaaaacg tattggcagc gacgaaatgt
    13381 gggatcgtgc tgaggcggac ctggcggttg cgctggaaga aaacaacatc ccgtttgaat
    13441 atcaactggg tgaaggcgct ttctacggtc cgaaaattga atttaccctg tatgactgcc
    13501 tcgatcgtgc atggcagtgc ggtacagtac agctggactt ctccttgccg tctcgtctga
    13561 gcgcttccta tgtgggcgaa gacaacgagc gtaaggtacc ggtaatgatt caccgcgcaa
    13621 ttctggggtc gatggaacgt tttatcggta tcctgaccga agaatttgct ggtttcttcc
    13681 cgacctggct tgcgccggtt caggttgtta tcatgaatat taccgattca cagtctgatt
    13741 acgttaacga attgacgcaa aaactatcaa atgcgggcat tcgtgttaaa gcagacttga
    13801 gaaatgagaa gattggcttt aaaatccgcg agcacacttt gcgtcgcgtc ccatatatgc
    13861 tggtctgtgg tgataaagag gtggaatcag gcaaagttgc cgttcgcacc cgccgtggta
    13921 aagacctggg aagcatggac gtaaatgaag tgatcgagaa gctgcaacaa gagattcgca
    13981 gccgcagtct taaacaattg gaggaataag gtattaaagg cggaaaacga gttcaaacgg
    14041 cgcgccctaa ccgtatcaat ggcgaaattc gcgcccagga agttcgctta acaggtctgg
    14101 aaggcgagca gcttggtatt gtgagtctga gagaagctct ggagaaagca gaagaagccg
    14161 gagtagactt agtcgagatc agccctaacg ccgagccgcc ggtttgtcgt ataatggatt
    14221 acggcaaatt cctctatgaa aagagcaagt cttctaagga acagaagaaa aagcaaaaag
    14281 ttatccaggt taaggaaatt aaattccgtc ctggtacaga tgaaggcgac tatcaggtaa
    14341 aactccgcag cctgattcgc tttctcgaag agggtgataa agccaaaatc acgctgcgtt
    14401 tccgcggtcg tgagatggcg caccagcaaa tcggtatgga agtgcttaat cgcgtgaaag
    14461 acgatttgca agaactggca gtggtcgaat ccttcccaac gaagatcgaa ggccgccaga
    14521 tgatcatggt gctcgctcct aagaagaaac agtaaggcct tcaagtagca ctgtctgtgg
    14581 agcctttggg cttcacagac attgttcgcc tttgtttcgt ttattaacaa tgcgaagtgg
    14641 aagttattaa aatgccaaaa attaagaccg tacgcggtgc tgctaagcgc ttcaaaaaaa
    14701 ccggtaaagg tggttttaag cacaagcacg ctaacctgcg tcacattctg accaaaaaag
    14761 cgaccaaacg taaacgtcac ctgcgtccga aagccatggt ttccaaaggc gatctgggcc
    14821 tggtaatcgc gtgcctgccg tacgcataag ccgttaacgt ttttaacttt ttaattagaa
    14881 tatagataca ggagagcaca tatggctcgc gtaaaacgtg gtgttattgc acgcgcacgt
    14941 cacaagaaaa ttttgaaaca agctaaaggc tactacggtg cgcgttctcg cgtataccgc
    15001 gttgccttcc aggctgttat caaagctggt cagtatgctt accgcgaccg tcgtcaacgt
    15061 aagcgtcagt tccgtcaact gtggattgcg cgtatcaacg cagcagcacg tcagaacggt
    15121 atttcttaca gcaaattcat caatggcctg aaaaaagcct ctgttgaaat cgaccgtaag
    15181 atcctggctg atatcgcagt attcgacaaa gtagcgttca ccgctctggt tgaaaaagcg
    15241 aaagcagctc tggcataagc cagttgaaag agggagctag tctccctctt ttcgtttcaa
    15301 cgccatcaaa acattgactt ttatcgccgt agccttttca ataaaggtct tttgaagagt
    15361 accaaaaggt aacgcaagca atgaatgctg ctattttccg cttctttttt tactttagca
    15421 cctgactcca ggaggctagc gcgtgagaag agaaacggaa aacagcgcct gaaagcctcc
    15481 cagtggaggc tttttttgta tgcgcgtttt gaaaaataaa aagcctgtct catcaggcgt
    15541 aattgtcgca gccagtctgg acacggccag cgcggagaaa ccggagcgta cttaagtacg
    15601 tgaggatttc gagcactgcc cgggaccaaa atggcaagta aaatagcctg atgggatggg
    15661 ctctaagtcc aacgaaccag tgtcaccact gacacaatga ggaaaaacat gtcacatctc
    15721 gcagaactgg ttgccagtgc gaaggcggcc attagccagg cgtcagatgt tgccgcgtta
    15781 gataatgtgc gcgtcgaata tttgggtaaa aaagggcact taacccttca gatgacgacc
    15841 ctgcgtgagc tgccgccaga agagcgtccg gcagctggtg cggttatcaa cgaagcgaaa
    15901 gagcaggttc agcaggcgct gaatgcgcgt aaagcggaac tggaaagcgc cgcactgaat
    15961 gcgcgtctgg cggcggaaac gattgatgtc tccctgccgg gtcgtcgcat tgaaaacggt
    16021 gggctgcatc cggtgacccg taccatcgac cgtatcgaaa gtttcttcgg tgagcttggc
    16081 tttaccgtgg caaccgggcc ggaaatcgaa gacgattatc ataacttcga tgctctgaac
    16141 attcctggtc accacccggc gcgcgctgac cacgacactt tctggtttga cgctacccgc
    16201 ctgctgcgta cccagacctc tggcgtacag atccgcacca tgaaagccca gcagccaccg
    16261 attcgtatca tcgcgcctgg ccgcgtttat cgtaacgact acgaccagac tcacacgccg
    16321 atgttccatc agatggaagg tctgattgtt gataccaaca tcagctttac caacctgaaa
    16381 ggcacgctgc acgacttcct gcgtaacttc tttgaggaag atttgcagat ccgcttccgt
    16441 ccttcctact tcccgtttac cgaaccttct gcagaagtgg acgtcatggg gaaaaacggt
    16501 aaatggctgg aagtactggg ctgcgggatg gtgcatccga acgtgctgcg taacgttggc
    16561 atcgacccgg aagtttactc tggttttgcc ttcgggatgg ggatggagcg tctgaccatg
    16621 ttgcgttacg gcgtcaccga cctgcgttca ttcttcgaaa acgatctgcg tttcctcaaa
    16681 cagtttaaat aaggcaggaa tagattatga aattcagtga actgtggtta cgcgaatggg
    16741 tgaacccggc gattgatagc gacgcgctgg caaatcaaat cacgatggcg ggcctggaag
    16801 ttgatggtgt agaaccggtt gccggtagct tccacggcgt ggtcgttggt gaagtggttg
    16861 agtgtgcgca gcatccgaac gctgacaaac tgcgtgtgac aaaagtgaat gtcggcggcg
    16921 atcgcctgct ggacatcgtc tgcggtgcgc caaactgccg tcagggcctg cgtgtggcgg
    16981 tagcgaccat tggtgctgtt ctgccgggtg atttcaaaat taaagccgcg aaactgcgtg
    17041 gcgaaccgtc tgaagggatg ctgtgctcct tctctgagct gggaatttct gacgatcata
    17101 acggcattat cgagctgcct gcggatgcgc cgattggcac cgacatccgc gaatacctga
    17161 aactcgatga caacaccatc gaaattagcg tgacgccaaa ccgtgccgac tgcttaggta
    17221 tcattggtgt tgcgcgcgac gttgccgtgt tgaaccagct gccgctggtt gaaccggaaa
    17281 tcgttccggt tggtgcgacc atcgacgaca cgctgccgat tgcagtagaa gcgccggatg
    17341 cctgcccgcg ctatcttggc cgtgtggtaa aaggcattaa cgttaaagcg ccaactccgc
    17401 tgtggatgaa agaaaaactg cgtcgttgcg ggatccgttc tatcgatgca gttgttgacg
    17461 tcaccaacta tgtgctgctc gaactgggcc agccaatgca cgctttcgat aaagatcgca
    17521 ttgaaggcgg cattgtggtg cggatggcga aagagggcga aacgctggtg ttgctcgacg
    17581 gcactgaagc gaagctggat gctgacaccc tggtcatcgc cgaccacaac aaggcgctgg
    17641 cgatgggcgg catatttggt ggcgaacact ctggcgtgaa tgacgaaacg caaaacgttc
    17701 tgctggaatg cgctttcttc agcccgctgt ctatcaccgg tcgtgctcgt cgtcatggcc
    17761 tgcatactga tgcgtctcac cgttatgagc gtggcgttga tccggcactg cagcacaaag
    17821 cgatggaacg tgcgacccgt ctgctgatcg acatctgcgg tggtgaagcg ggtccggtaa
    17881 ttgatatcac taacgaagca acgctgccga agcgtgcaac catcacttta cgtcgtagca
    17941 aactggatcg cctgatcggc catcatattg ctgatgagca ggtaacagac attctgcgtc
    18001 gtctcggctg cgaagtgacc gaaggcaaag acgagtggca ggcagttgcg ccgagctggc
    18061 gtttcgacat ggagattgaa gaagatctgg tcgaagaagt cgcgcgtgtt tacggctaca
    18121 acaacatccc ggatgagccg gtacaggcaa gcctgattat ggggactcac cgtgaagctg
    18181 acctgtcgct caagcgcgta aaaacgctgc tcaacgacaa aggctatcag gaagtgatca
    18241 cctacagctt cgttgacccg aaagtgcagc agatgatcca tccaggcgtt gaagccttac
    18301 tgctgccaag cccgatctct gttgaaatgt cagcaatgcg tctttccctg tggaccggcc
    18361 tgctggcaac cgtggtgtac aaccagaacc gtcagcagaa tcgtgtacgc attttcgaaa
    18421 gcggtctgcg tttcgtgcca gatactcagg caccgttggg tattcgtcag gatctgatgt
    18481 tagccggtgt gatttgcggt aaccgttacg aagagcactg gaacctggca aaagaaaccg
    18541 ttgatttcta tgatttgaaa ggcgatcttg aatccgttct cgacctgacc ggtaaactga
    18601 atgaggttga gttccgtgca gaagcgaatc cggcactgca tccggggcaa tccgcagcga
    18661 tttatctgaa aggtgaacgt attggttttg ttggggttgt tcatcctgaa ctggaacgta
    18721 aactggatct taacggtcgc actctggtgt tcgaactgga gtggaacaag ctcgcagacc
    18781 gcgtggtgcc tcaggcgcgc gagatttctc gcttcccggc gaaccgtcgt gacatcgcgg
    18841 tggtggtcgc agaaaacgtt cccgcagcgg atattttatc cgaatgtaag aaagttggcg
    18901 taaatcaggt agttggcgta aacttatttg acgtgtaccg cggtaagggt gttgcggagg
    18961 ggtataagag cctcgccata agcctgatcc tgcaagatac cagccgtaca ctcgaagaag
    19021 aggagattgc cgctaccgtc gccaaatgtg tagaggcatt aaaagagcga ttccaggcat
    19081 cattgaggga ttgaacctat ggcgcttaca aaagctgaaa tgtcagaata tctgtttgat
    19141 aagcttgggc ttagcaagcg ggatgccaaa gaactggttg aactgttttt cgaagagatc
    19201 cgtcgcgctc tggaaaacgg cgaacaggtg aaactctctg gttttggtaa cttcgatctg
    19261 cgtgataaga atcaacgccc gggacgtaac ccgaaaacgg gcgaggatat tcccattaca
    19321 gcacggcgcg tggtgacctt cagacccggg cagaagttaa aaagccgggt cgaaaacgct
    19381 tcgcccaaag acgagtaatc tgatctaact aaaaaggccg ctctgcggcc ttttttcttt
    19441 tcactgtcga agagtcaccg taaaatcaac gccatgacac ttcagcagaa cggataccat
    19501 gctgacactt gcccgccaac aacagcgaca aaatattcgc tggttattat gcttgtcagt
    19561 tttgatgcta ctggcgcttc tcttaagcct ttgcgccggt gaactatgga ttttgccagg
    19621 tgactggttt tctcctcgtg gcgaactgtt cgtctggcaa attcgcctgc cacgtacgct
    19681 ggctgtattg ctggttggtg cggcgctggc tatatccggc gctgtaatgc aggcgttgtt
    19741 tgaaaatcct ctggcagaac ctggactact tggcgtctct aacggcgcag gcgtggggct
    19801 tatcgccgcg gtattacttg ggcaagggca actccccaac tgggcgctag ggctgtgtgc
    19861 gattgctggc gcgcttatca tcactttaat actcttacgt ttcgcccgtc gtcatctttc
    19921 gaccagtcgg ttattgctgg ctggcgttgc attagggatt atctgtagcg cactaatgac
    19981 gtgggctatc tacttttcca cctctgttga tttacgtcag ctgatgtact ggatgatggg
    20041 cggttttggc ggcgtagact ggcggcaaag ctggctgatg ctggcattga tcccggtgtt
    20101 gttgtggatc tgttgtcagt ccaggccgat gaatatgtta gcacttggcg agatctcggc
    20161 gcggcaactg ggtttacccc tgtggttctg gcgcaatgtg ctggtggcag cgaccggctg
    20221 gatggttggc gtcagtgtgg cgctggcggg tgctatcggc tttattggtc tggtgatccc
    20281 acatattctc cggctgtgtg gtttaaccga tcatcgcgta ttacttcccg gctgcgcgct
    20341 ggcaggggcg agcgcattgc tgctggccga tattgtagcg cgcctggcat tagctgccgc
    20401 agagctgcca attggcgtgg tcaccgcaac gttgggtgcg ccggtgttta tctggttatt
    20461 gttaaaagca ggacgttagc cgcaaaacac ggtctatgat taaaagctaa gatttttact
    20521 gaccataccc aggagaaacg atgcaagatt ccattctgac gaccgtagtg aaagatattg
    20581 acggtaaagt gaccacgctg gagaagtacg ccggtaatgt gctgttgatt gtcaatgtcg
    20641 cctcaaagtg tggcttaacg ccgcaatatg agcagttgga gaatattcag aaagcctggg
    20701 ctgatcgtgg ttttgtggtg ctgggattcc cgtgtaacca gtttctggag caagaaccgg
    20761 gtagcgatga agagattaaa acttattgca ccaccacatg gggggtgacg ttcccgatgt
    20821 tcagtaagat tgaagttaat ggcgaaggac gtcatccgct gtatcaaaaa ttgattgccg
    20881 cagcgccgac tgcagtcgcg ccggaagaga gcggattcta tgcccgaatg gtcagcaaag
    20941 gtcgtgcacc gctgtaccct gatgatattt tatggaattt tgagaaattt ttagttggca
    21001 gggacggatt ggtcatccag cgtttttccc cggatatgac gccggaagat cccattgtga
    21061 tggaaagcat taaactggcg ttggcaaaat aatgtctatt gtgatgcagt tacaggatgt
    21121 tgcggaatct acccgcctgg ggccgctttc tggcgaggtt cgggctgggg agatcctgca
    21181 cctggtgggg ccgaatggcg cgggtaagag taccttactg gcgcgaatgg ccggaatgac
    21241 cagcggtaag ggaagcattc agttcgcggg gcaaccactg gaagcatggt ctgcaacaaa
    21301 gctcgcgctg catcgcgcct atctttcaca acagcagacg ccgccgtttg caatgccggt
    21361 ctggcactac ctgacactgc atcagcacga taaaacgcgt accgaactac tgaatgatgt
    21421 cgcaggggcg ctggctcttg atgacaaact cggacgtagc accaatcaac tttccggcgg
    21481 tgaatggcaa cgcgtacgtc ttgctgcggt ggtgctgcaa atcacaccac aagccaatcc
    21541 cgcaggccaa ttgctgcttc ttgatgagcc gatgaacagt cttgatgttg cgcaacaaag
    21601 tgccttagac aaaattctga gcgcgctgtg tcagcaagga ctggcgattg tgatgagcag
    21661 tcacgatctc aaccacacat tgcgtcatgc gcatcgggcg tggttgctaa aaggtggaaa
    21721 aatgctggcc agtggacgca gagaagaggt gctcacgccg gcaaatctgg cgcaggccta
    21781 tgggatgaat tttcgccgtc tggatatcga aggtcacaga atgctgattt cgaccatctg
    21841 atttcattag tatcttgaaa atcgggtaat tacgacgcta aattaatgca agaataaaaa
    21901 acagaggaac cgtacggaat gcgtttctgc cttattttga tcacagcact gtttctggcc
    21961 gggtgtagcc accataaagc accgccgcca aatgccagac tttctgattc gattaccgtt
    22021 attgccggtt tgaacgacca gctacaaagc tggcatggca cgccgtatcg ttatggtggc
    22081 atgacgcggc gcggtgtgga ctgttcggga tttgtggttg tgacgatgcg cgatcgtttc
    22141 gatttgcagc tgccccgtga aaccaaacaa caagcctcta ttggcacgca aattgataaa
    22201 gacgagttgc tgccaggtga cctggtcttt ttcaaaacgg gttccggaca aaatggtttg
    22261 catgtaggta tttacgatac caacaaccaa tttatccacg cctctaccag caagggagtg
    22321 atgcgttcct cacttgataa tgtctattgg cagaaaaatt tctggcaggc gagacgaatc
    22381 tagtatgtcg aaaaatggac ggcgatgata catcgccgtc catttactcc tgaatacagt
    22441 agtattttgc agacaaaaat tccgtaaatg gattcttcgt ttataatcag gataatctaa
    22501 aaaaaggacc cctgaaaaag tggttaacat ggaacaaatg aaagttactc cttctgtaat
    22561 tgttatgtct tatggcctca tcccttttag ccggatactg aaaaacatcc ttcgagaggg
    22621 acggttacca tgaagatttt tttggagaat ctttatcatt ctgattgcta tttcctgccg
    22681 ataagagata accagcaaga ccttgtcggc gttgagttaa ttacccattt ctccagtgaa
    22741 gatggtacgg ttcgtatccc gacgagtcgg gttatagcgc aacttaccga ggagcaacat
    22801 tggcaattat tttctgaaca gttggaatta ctaaaatcat gccaacactt ttttattcaa
    22861 cataaactct ttgcctggct taatttaacg ccacaagtgg ctacattatt attagacagg
    22921 gataatttcg caggcgaact attaaaatat ccgtttattg aactgttgat taatgagaat
    22981 tacccgcatt tcaatgaagg aaaagataat cgcgatcttt tatccttatc gcaaatgtat
    23041 ccccttgtgt taggtaattt gggagcaggt aatagcacaa tgaaagcggt gtttgatggt
    23101 cttttcaccc gcgtcatgct ggataaaagt tttattcagc aacaaatcac gcatcgctcc
    23161 ttcgaaccgt ttattcgtgc gatccaggcg caaatttccc cttgttgcaa ttgcatcatc
    23221 gcggggggca ttgatacgcc agaaatattg gcacagatta tcccctttga tttccatgct
    23281 ctccagggat gcctgtggcc tgcggttcct ataaatcaga taacgacgtt ggttcagcga
    23341 taaccctcct gtttgctggt gtttaagacg agagtaaccg tctacactat caaacaggag
    23401 gatctatgac cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag
    23461 cactttcccc tacgccttta aataatgccc ggctgatttg gcataatacc gaactggcta
    23521 acacgctgag tattccatcg tcgctgttta aaaatggcgc aggtgtctgg ggcggcgaaa
    23581 ccctactgcc tggtatgtca ccacttgcgc aggtttacag tggtcatcag tttggcgtct
    23641 gggcgggcca actgggtgat gggcgcggca ttttactcgg cgaacaactg cttgctgatg
    23701 gcactacaat ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg
    23761 atggacgggc ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt
    23821 atctgggcat tccgacgacc cgcgctttaa gtatcgtcac cagcgattcg ccagtgtatc
    23881 gggaaacggt ggagtcaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg
    23941 gtcatttcga acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttagctgact
    24001 ttgccattcg tcattactgg tcacatcttg cagatgagga ggacaaatac cgtctctggt
    24061 ttaccgatgt tgtcgcacgt accgcctcgt taattgccca atggcagacg gtcggctttg
    24121 ctcatggggt gatgaatacc gacaacatgt cgctgctggg gctgacgctt gattacgggc
    24181 cgtttggttt tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag
    24241 ggcgttacag ctttgataat caacctgccg tcgcgttgtg gaatttacag cgtctggcgc
    24301 agacattgtc gccatttgtt gccgtagatg gcctgaatga ggccctggac agctatcagc
    24361 aggttttgtt gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc
    24421 aaaaagagga taacgcgcta cttaatgaat tattcagtct gatggcgcga gagcgcagcg
    24481 attatacccg cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc
    24541 tacgtgatga gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc
    24601 gtttgcaaca agacgagatt accgatagtg agcgtcagca actgatgcaa agcgttaacc
    24661 cggctctggt gttgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg
    24721 atatgacgga attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg
    24781 atgactatgt cagtcgtcca cccgactggg gtaaacggct ggaagtcagc tgctcgagtt
    24841 aaaggttact tggtcaacag cagcttgcca gcctgagttt tacggagcag atattcttgc
    24901 ccgtcatgat cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc
    24961 cggatgggtt gcggggaagc ggtcttgtga ctggcatccg tttcaggcgt gagttttctg
    25021 ctatccgtat aacgcataaa aattaataat aatttatata acaatgattc ttattatcaa
    25081 tattatggcg ggccagagca agcagtaatt tgttgagaag acaacaagtt gcgcagaaat
    25141 gcccatcccg tcaggaatgg gcacgcattc agaagcgagt atctaccgca gaggcgagtt
    25201 tttcgaccag gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt
    25261 aagtgagcgg ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca
    25321 taattccagc aatcgccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt
    25381 ctaactggcg acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt
    25441 caggtaaatc aaactcgtgc agcgtatcgc aggctgcggc gatatcctcg gcatgataat
    25501 tcggtttttt gccaccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga
    25561 tggtcatctg accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga
    25621 tagcatccac ggcaatccgc gtgttgccat cggtaccatt tttaaaaccc accggacagg
    25681 agagtgccga agccatttcg cggtggatct gactttcggt agtacgtgcg ccaatcgcgc
    25741 cccaactgat taaatcagca ataaactgac cggtcaccat atcgaggaac tcagtcgcgg
    25801 tagggacgcc cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat
    25861 ttacccgata gctaccgttt aaatctggat cggagatgag tcctttccag ccgacaaccg
    25921 ttcgtggttt ttcaaaatag gtgcgcatta cgatttccag ccgtggctgg tactggttgc
    25981 gtagcgactg cagacgggtg gcgtactcca tcgcagcggt gagatcgtgg atcgagcagg
    26041 ggccaataat gaccaacagt cgcttatctt caccattcag tattttttca attctacggc
    26101 gggagtcggt gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg
    26161 cgggcgttac caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa
    26221 gtctcagaaa tttgttgctt ctacggcagg actaccggga agtgatgcgc atcacgataa
    26281 accaatcgca gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga
    26341 taacaaaaaa gtaagccagt acactagttc tctagtacat tcggcggcta aggcccatga
    26401 tatcgaggat cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga
    26461 tctgattttt acggtacaac gcttccactt ccgcgacttc catcctgcac tgacgaagcg
    26521 aggcatagcg gctgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga
    26581 tagtcaggcc gaacaatttg tgctgaagtg gtttgagcga cgcgggtagc accagattat
    26641 ccatatcgtc ggcaataaag gggtaatttg ccgcgcggat accaaattgc attgccagat
    26701 acagactggt gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctggt
    26761 ccagattgcg caacgaaata ccgtcatcgt gggcaagggt gtaatcaatc gccgcaatgc
    26821 gcgcatcata tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg
    26881 ttggatccag tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc
    26941 aaaagccttc actttgcaag atgatagcgc gaatctccgg caacacgatg gagtagaaga
    27001 ccagcgggcg cacacctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg
    27061 cacggctctc attttcgaca aacggcagcg taatgctgct gatagtgacg ggaaattgtg
    27121 acattactgc gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa
    27181 cgtggcgatc aacagcatta tccattttgt tcttcccgtg atgcagacat aattctatga
    27241 aagcataaat taaaaacgca cagaagcgta gaacgttatg tctggtttat aaaatgaaac
    27301 ttaaatttta ttttttatga aaacagcatt tcatttttat ggtttcgttt ataccgatgg
    27361 tttatgtgga aattgtcgaa gagagca
//
LOCUS       DAZGJT010000062        27207 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZGJT010000062 DAZGJT010000000
VERSION     DAZGJT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.062"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHKFK_002878"
     CDS             complement(46..1905)
                     /gene="gss"
                     /locus_tag="ACHKFK_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO9053170.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHTPLPQGQQ
                     WTRELEMVVENGGYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALQQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDQTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKE"
     gene            2110..2976
                     /gene="yghU"
                     /locus_tag="ACHKFK_002879"
     CDS             2110..2976
                     /gene="yghU"
                     /locus_tag="ACHKFK_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO9053171.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFASINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTEAMSWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERLAVKRGRIVNRINGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3109..3357)
                     /gene="hybG"
                     /locus_tag="ACHKFK_002880"
     CDS             complement(3109..3357)
                     /gene="hybG"
                     /locus_tag="ACHKFK_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO9053172.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3370..3711)
                     /gene="hypA"
                     /locus_tag="ACHKFK_002881"
     CDS             complement(3370..3711)
                     /gene="hypA"
                     /locus_tag="ACHKFK_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO9053173.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3704..4192)
                     /gene="hybE"
                     /locus_tag="ACHKFK_002882"
     CDS             complement(3704..4192)
                     /gene="hybE"
                     /locus_tag="ACHKFK_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO9053174.1"
                     /translation="MTEEIAGFQTSPKAQVQVAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4185..4679)
                     /gene="hybD"
                     /locus_tag="ACHKFK_002883"
     CDS             complement(4185..4679)
                     /gene="hybD"
                     /locus_tag="ACHKFK_002883"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO9053175.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     ATHD"
     gene            complement(4679..6382)
                     /gene="hybC"
                     /locus_tag="ACHKFK_002884"
     CDS             complement(4679..6382)
                     /gene="hybC"
                     /locus_tag="ACHKFK_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO9053176.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPAKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6379..7557)
                     /gene="hybB"
                     /locus_tag="ACHKFK_002885"
     CDS             complement(6379..7557)
                     /gene="hybB"
                     /locus_tag="ACHKFK_002885"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO9053177.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7547..8533)
                     /gene="hybA"
                     /locus_tag="ACHKFK_002886"
     CDS             complement(7547..8533)
                     /gene="hybA"
                     /locus_tag="ACHKFK_002886"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO9053178.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSEHVQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8536..9654)
                     /gene="hybO"
                     /locus_tag="ACHKFK_002887"
     CDS             complement(8536..9654)
                     /gene="hybO"
                     /locus_tag="ACHKFK_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO9053179.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMVESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9843..10130)
                     /gene="yghW"
                     /locus_tag="ACHKFK_002888"
     CDS             complement(9843..10130)
                     /gene="yghW"
                     /locus_tag="ACHKFK_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO9053180.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10249..11136)
                     /gene="yghX"
                     /locus_tag="ACHKFK_002889"
     CDS             complement(10249..11136)
                     /gene="yghX"
                     /locus_tag="ACHKFK_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO9053181.1"
                     /translation="MPRLTAKAFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVTRRVAKEGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGISGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHYAELDSRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11293..12333
                     /gene="gpr"
                     /locus_tag="ACHKFK_002890"
     CDS             11293..12333
                     /gene="gpr"
                     /locus_tag="ACHKFK_002890"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO9053182.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLREWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLHEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12373..12867)
                     /gene="yqhA"
                     /locus_tag="ACHKFK_002891"
     CDS             complement(12373..12867)
                     /gene="yqhA"
                     /locus_tag="ACHKFK_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO9053183.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13058..13942
                     /gene="yghA"
                     /locus_tag="ACHKFK_002892"
     CDS             13058..13942
                     /gene="yghA"
                     /locus_tag="ACHKFK_002892"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO9053184.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(13981..14406)
                     /gene="exbD"
                     /locus_tag="ACHKFK_002893"
     CDS             complement(13981..14406)
                     /gene="exbD"
                     /locus_tag="ACHKFK_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO9053185.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14413..15147)
                     /gene="exbB"
                     /locus_tag="ACHKFK_002894"
     CDS             complement(14413..15147)
                     /gene="exbB"
                     /locus_tag="ACHKFK_002894"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO9053186.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15399..16586
                     /gene="metC"
                     /locus_tag="ACHKFK_002895"
     CDS             15399..16586
                     /gene="metC"
                     /locus_tag="ACHKFK_002895"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO9053187.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALGFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16726..17385
                     /gene="yghB"
                     /locus_tag="ACHKFK_002896"
     CDS             16726..17385
                     /gene="yghB"
                     /locus_tag="ACHKFK_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO9053188.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYSLSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17425..18324)
                     /gene="yqhC"
                     /locus_tag="ACHKFK_002897"
     CDS             complement(17425..18324)
                     /gene="yqhC"
                     /locus_tag="ACHKFK_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO9053189.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18518..19681
                     /gene="yqhD"
                     /locus_tag="ACHKFK_002898"
     CDS             18518..19681
                     /gene="yqhD"
                     /locus_tag="ACHKFK_002898"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO9053190.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPSRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19786..20613
                     /gene="dkgA"
                     /locus_tag="ACHKFK_002899"
     CDS             19786..20613
                     /gene="dkgA"
                     /locus_tag="ACHKFK_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO9053191.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20813..21739
                     /gene="yqhG"
                     /locus_tag="ACHKFK_002900"
     CDS             20813..21739
                     /gene="yqhG"
                     /locus_tag="ACHKFK_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO9053192.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTSQTLKMVAENGRWVIGDIVSNHGSVLQAVNSENEKTLA
                     AIASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYYNPICFGEESLFSRVDEIRVLEKTTDSARIHVRFTLTNGNNEEQELILQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21790..22047
                     /gene="yqhH"
                     /locus_tag="ACHKFK_002901"
     CDS             21790..22047
                     /gene="yqhH"
                     /locus_tag="ACHKFK_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO9053193.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKINQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22089..24308)
                     /gene="ygiQ"
                     /locus_tag="ACHKFK_002902"
                     /pseudo
     CDS             complement(22089..24308)
                     /gene="ygiQ"
                     /locus_tag="ACHKFK_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
     gene            24560..25309
                     /locus_tag="ACHKFK_002903"
     CDS             24560..25309
                     /locus_tag="ACHKFK_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO9053194.1"
                     /translation="MEQVITKRRYYDIGLQIEELLYSGVFKAGERLPSERELSERFNT
                     SRTTIREAIIMLELKGVLNVKQGSGIFFVDSTDKLNQKSLMPYSEIGPFELLQARQVI
                     ESNITGFAASQISFNELQELKKIIGLQEKAIAAESDKFEDLDHRFHSIIAEATQNRVL
                     IKQAAELWRAVRTENPRWKKLNYKYLHEKHLRLQWLEDHRAIFLALQQKDSELAREAS
                     WRHLENSKNELIKIFKQDASISDFDDFFFAR"
     gene            25634..27106
                     /locus_tag="ACHKFK_002904"
     CDS             25634..27106
                     /locus_tag="ACHKFK_002904"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9053195.1"
                     /translation="MSGNESIDAAHYDQVTTWPRDELQADIVHIGFGAFHRGHQAVYT
                     DLTNQLSDTHWGIFEINLFGDAQLIENLNAQNGLFSVVETSASQSTSRLVRSVTGGIH
                     TPVDGIAAAIQKLTEPQVKIVSLTITEKGYCLDSQTRSLDLTNGLIKHDLQNPDAPQS
                     AIGLIVCALQQRKAAGLAAFSVLSCDNLPDNGHLTRNAVLGFARQLDQPLAQWIEENV
                     SFPGTMVDRIVPAMTESQFALLETKTGYADPCGIVCESFRQWVIEDNFVRGRPEWDKA
                     SAMFVSNVQPYEEMKLRMLNGSHSFLAYNGSLAGYEFIWQCMEDANFRSITRQLMINE
                     QARTLNPDLNINIQEYADLLIERFSNRNVAHRTGQIAMDGSQKLPQRALTPWLKLHQQ
                     KQNNAVLSLLVAGWLHYVIDAVEKSQSVADPMNDQLQALIKEQQDAWQQALALLHLSA
                     IFGDLSNHQPFINEIKIAFANIKNKGIKATISQLLSDEQK"
     gene            27103..>27207
                     /locus_tag="ACHKFK_002905"
     CDS             27103..>27207
                     /locus_tag="ACHKFK_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate oxidoreductase"
                     /protein_id="HFO9053196.1"
                     /translation="MKTLICQQPGVMEYVEKDIPTPADNEVLLKIKAVG"
BASE COUNT         6855 a         7151 c         6741 g         6460 t
ORIGIN      
        1 tactgattat tcttcataat cagtacgggg ttgctacaac atgtattact ctttcaccac
       61 aattaacggt tcaatatcac tctctttttt gatgaccagt gattcatcac cgcgcaaaca
      121 cgtcccaccg tagttgccgc caacggtgaa agtacatacc tgaatgtatt taccgtccac
      181 tttcggcaaa caccacagtt gctggtaaat atttttctgt tcggcaaatt taccactggt
      241 ctgatcaagc acctcttcat gatggctgac caggtcaatg ttgctaccac agcgcccggc
      301 aatcggtttc acggcgtaac cggttttcac cagttcatca ttaacagtga aatcggtatc
      361 cagcagataa cgatgatgcg ggaacagcga ccagagaatt ggcagaatcg ctttgttgcc
      421 agggatcacc gtccaaagcg gttcaaagac cagcacttcc ggacgtagca atacgtcgat
      481 aagacgcact tcattttgcg ggtgaccagt gcgaatcggg accgcagcaa attcacggtc
      541 gctaacttca cgaatctgat caaacgccgt ttcccacgcc caggtcttcc acacgcagtt
      601 aaccagtcgc ccttccccat caatcagttg cccggcagca tcccagccca gttcatctag
      661 cccgcgcaag atacgcgttt caaagcccgc ctgttgcagc gcctgctcca taaactgagc
      721 gtgatagttc tcttcaatat ctttgtcctg catgatatgg acaaacggac gcgcgcgact
      781 gtgtttccag gcaccagcca gttcgttaat cagcccttcc gcaggattga agccgttgcc
      841 tttatagccc tgctccgccc aacgttcgag gatcaagccc gcttcggtat gacaggaggc
      901 agaatcggcg ttgtattcgt agactttcag gccacgttca tccatgcaga aatccatacg
      961 accggtgatc atatggtgac ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat
     1021 tttcggaatg tcgaacaacg ccagcaggtt gtcatctttc agcaccttgt cggtggcgtg
     1081 aagatacatc aggtgcagct cgttggtggc tttaatcagc tcctgctcgg cgctctcggt
     1141 aatggtgtag taatgatacg gatcctgatt gatcacctga ccgttggcct gtacataggc
     1201 gttttgcagc ggatcttttt catccagcca tttaccgtca aactggcctt tgttttccag
     1261 acgcgccccg ctgattttca gcagttcgcc tgcaatttcc ggctgtggca agctgtattc
     1321 ggtatcttcc gtctggatca tccagcccag aatagtggtg tcatcgaagg tgtctttcag
     1381 ggtatagccg ccgttttcga ccaccatctc cagctcgcgt gtccactgtt gcccttgcgg
     1441 caacggggta tgaatcacgt tctgttccgc aatacggact ttgttgccat gcaattgggt
     1501 aatgatggcg acatggccag tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc
     1561 ccccgctacc ggtgcacgcg gcgaaccgtt aggaaatgcc tgcaatggca ggatgttgtc
     1621 attgaccact tcacgcagga agcgcagtga gaaaatctcc catgccatac ccacgtcagt
     1681 gaagactaca ccgtagttca gaaagagaaa acggcgagca aattcaacgc attgccattt
     1741 gtggcccata tattcatcgt cgatatagct acggaatacg gcgtcatctt cgtattcctg
     1801 cggatcgaga gaactgtaat ctgaagagta gattgctacc ccacctgggg cgtagcccaa
     1861 taatgtcccg aacggggcat cctggctggt cgttcctttg ctcatcactt tacctttaac
     1921 aatacaacct aagcagttgg cgtaattgtt gtgctctgat tacctgctca gcgacattaa
     1981 ccggacagag gtcagactaa tcatacacct catcgcagcg gctggcgcaa agggttaaaa
     2041 aattcacatt ctgtacagca agtgacctgc tacactgctt caacactacc actcagaagg
     2101 caactcacta tgacagacaa tacttatcag cccgcgaaag tctggacgtg ggataaatcc
     2161 gctggcggcg cgttcgccag tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg
     2221 ctgccggtgg gcaaacatcc attgcaactt tattcgctgg gaacgccgaa cggtcagaaa
     2281 gtaacgatta tgcttgagga gctgctggcg ctgggcgtta ctggtgcaga gtatgacgcc
     2341 tggctgatac gtattggcga tggcgatcaa ttctccagcg gctttgtcga agtgaaccca
     2401 aattcgaaga tcccggcatt gcgcgatcat acccataatc cgccgatccg cgtgtttgag
     2461 tctggatcga ttctgcttta tctggcagag aaatttggct acttcttgcc gcaggatctg
     2521 gcgaaacgta ctgaagcaat gagctggctg ttctggttac agggcgcggc accgttcctc
     2581 ggcggtggtt ttggtcactt ttaccattac gcaccggtga aaattgagta cgccatcaac
     2641 cgctttacta tggaagccaa gcgccttctc gacgtgctgg ataagcaact ggcgcagcat
     2701 aagtttgttg cgggcgatga gtacaccatt gcggatatgg cgatttggcc atggtttggc
     2761 aatgtggtgt taggcggcgt atatgatgcc gcagagtttc ttgatgcggg cagttataag
     2821 catgtacaac gctgggcgaa agaagtgggc gaacgtctgg cagtgaagcg tgggcgtatt
     2881 gttaaccgca tcaacggacc gctgaacgag cagttgcatg aacgccatga cgccagtgat
     2941 ttcgagacga atacggaaga taagcgtcag gggtaatggt tagcgttcgt cgcagcaagc
     3001 catccagacc aatgtaggcc ggataaggcg ttcacgccgc atccggcaat cgagcacaat
     3061 gcctgatgcg acgctgccgc gtcttatcag gcctacaaaa gctaatcatc acgcgctggt
     3121 aatgtcgtaa tccatttggc gcagtgcgtc taatgtggct ttggcttcgt cttcatcgat
     3181 aatgctcatg gcaaatccga cgtgtaccag tacccactgg cccagcagat cggcagggtt
     3241 accttcacaa atcagggcga tattcacgtc gcgcttgata ccacatactt caacctgcgc
     3301 aagctggtga atatcttcac cgacagccag cacctggcct ggaacgccaa tacacattgt
     3361 taactccggt tattcaactt caatactttt gacgattagc gaatcgccgg tatcgacacg
     3421 caaccgctcg ccatgacaga tcggacactg cgcatcgtgc tgatgaatct ccaccacctg
     3481 gctgcaatcc caacaccagg cctgggcggg tttatagacg atatgtaaat cgcacccttg
     3541 cgctaccgtt ccgtggcaga caatttcaaa actaaaacgg acggcgctct cctcaacgca
     3601 ggagagtgca ccaatttcca gccacacggc ggtgacgcgc ttaacatcgt gctgctccgc
     3661 ctgccgttgg ataatttcaa cggcgctctg gcaaagcgac aactcatgca ttttcgccac
     3721 tcctgcggcc aaatagcagg gcacgacgcc ctgcgtgtgg tacatccgga ttggtgactg
     3781 gcagcgaaag gatcattcgg gcgcagtcat ccgtcagccg ttggccctct tcaatcgaca
     3841 tgctgtgcga aagcggcgac atcagcgagc aggagaggta ttgcgaaaca ccgtccagtt
     3901 caccaacagt aaaggtcata gtgccatacg gcagttgcag accaattttt tcactcactt
     3961 tgcgcagcgg ccagagttga tccgggccgg ggaagataac tgcactcagc atccacgggg
     4021 tgatcacaca ccccgtccac tgaccttcga acagcgtaaa gtcagaaaca tacaccggca
     4081 ttgaaggatg cagaaaagag agatcatgca tcgaacgccg ggcaatttct tcaaaagcta
     4141 cctgtacttg cgccttcggg gaggtctgga aacctgctat ctcttcagtc atgagtcgcc
     4201 tcccgtggga tagcttccac gcctgattcg cgcagcgcag ccagaacctg ctcaagcgca
     4261 ggttcaatca tcgcttcgac cgtcggtgtc aaaccgatgt gtggctccag tgattccggg
     4321 atcacgccga ccagggtcag ctttttcgga aactcgccgg tgaagcgcag ggccgacaat
     4381 acgtcggcca ggccaagctg atgcggagag attttgttgg taaacaacgc cggaacttct
     4441 tcatcccgca ggatcatcat cgttcccggt gcgttctttt tcgacacaat ggcatccgca
     4501 ataatcagat gatcgcgatt tgccatgtcg ccaagcagct ccattcccgc cgtgccgcca
     4561 tcgaggatct caacataatc cggcagaatg tatcgttgct ctaacgcttc aacaatccgc
     4621 acaccgatgg cttcatcggt cagcaaaata ttgccgaccc ctaagactaa aatacgcatt
     4681 acagaacctt cactgaaacc acttcgttgc cgtcggcatc cactacgtgt accgcacagg
     4741 ccatgcacgg gtcgaaggag tgaatggtac gcaccacttc cagcggttta ttcggatcgg
     4801 caaccggtgt acccaccagc gactgctcgt aaggaccgac gtcatcattg aagttacgcg
     4861 ggccagagtt ccaggttgat ggaacaactg cctggtagtt gctgatgata ccgtctttga
     4921 tcaccatcca gtgagagagc ataccgcgcg gcgcttcaag gaagccaacg cctttgaact
     4981 cgcccgttgc cggaatgttc ggcttcacaa aggtggtgtg atcgcctttg ccgatattgg
     5041 tgatcagtgc actgtattga ttttgcagga tatcctgcaa ttcgcagcag tgaacggtac
     5101 gaccaataat acggcccagc gtggagtgca gttgcgctac ttccagcgtg ttgccggtca
     5161 gtttctgata aatcgcaacg atttcattca gtttgttctg ggtagattcg cgacctgccg
     5221 ccagtttcac cagcatatta gccagtggac ccacttctac cgttttgccg tagaaagtcg
     5281 gtgatttcac ccaggaatat ttaccgtcgt cagaccaacc gtcgtaagcc ggaatggtgg
     5341 taccttccca cggtgcctgc ggcgcttcgt ctttatacca ggagtgcttc gcgctttcct
     5401 gaattccttt gatcaggtat tcatcggaat gagaagtgat cggacgatac gaggacagat
     5461 ccgcattctc aatgtagcca cccgggaaca ggaagctgcc atttttactg tcggtcggga
     5521 attccggcac gctcaggtag ttcaccgcac ctttaccgcg cgtcagccat tccgggtaga
     5581 acgcagcgat aactgcagta tcaaccttgt aaacctgctc aacaaagtcg ctcagcttgt
     5641 cgatgaagga cttgatgtac atcaggcgct caaggttcag cacacccaga ccgtcgaggt
     5701 tgatcgggtt cgcgacacca cctaccgcca ggttctgaat atgcggcgtt ttaccgccca
     5761 gcagcgccac aacgcggtta gcgtcacgct ggcactccag cgcctgcagg tagtgcgcta
     5821 ccgcaatcag gttcacttcc ggcggcagtt tcattgccgg gtgaccccag tagccattgg
     5881 cgaaaatacc caactgaccg ctggcaacca gatctttgat cttgttctga actttggtga
     5941 actcttccgg gctgttcaga tgccaggtcg aaacgccttt cagcatttcg gaggctttgg
     6001 ctggatcagc ttgcagtgca gaagtgatgt ccacccagtc cagcgccgaa agctgataga
     6061 aatgaacaat atggtcatgc gtggtgtgcg cagccagaat gatgttacgg atgtattgcg
     6121 cgttaaccgg aacgtcgata ttcagcgcac tttctgccgc acgaacggaa gacagcgcgt
     6181 gagtggtagt acatacgcca cagatacgtt gcacaatcat ccatgcatcg cgcggatcgc
     6241 ggtttttcac gatctcttcc atgccgcgcc acatggtacc ggaagcccat gctttcgaaa
     6301 cgacgccatt ttcgatttcg caatcgatgc gtaaatgccc ttcaatacgg gttaccggat
     6361 caatagtaat tctctggctc atgctttgct cgcctcatga cgattatgat cgttttgttt
     6421 taaaggagga agtatcggca gtagacgaat gagtacgata taagcgcaaa tctcaatagc
     6481 cacaaaacca atagaaatca acagttcttc ccaggtcggg aagtaggcgt aaccgccacc
     6541 cgggttaaat gccaccagcg aataggtcag acgccaggtt gcacaaccta acagtgcact
     6601 cagtgctgac aggaacagca tgcgggaatc attacgcaac ttcgccacac gcagaacgac
     6661 cagcgggaag agcatcagca ggacttcaat ccagaacatc acggagtaga agtcaccggc
     6721 aaacgctaac gacagcttgt cgcgatagat aagctcgcca aagcgcagca cgatgaaaat
     6781 cgccagcaac acactgatgg tgttggtcag cttaacgaac agactctttt catccggacc
     6841 gttgccacgc agacccgcct gcaccaacga accttcaaag atgacaatcg agaagcccat
     6901 gatgaacgcc gtcagcagcg agaacagcgg caacatttca tagctttgcc acagcggatg
     6961 caccttgtag cccgccgaga tcatcagcga ccccattgaa gactggtgca tagttggcag
     7021 cagcgcacca agcgcgatga tgaagaacat caccttgttc agacgtttga gtgacacctt
     7081 ccagcccaga cgttcaaaca atgccggagc aaactccagt gccatcacac cgatgtagat
     7141 ggtcatacag accgccgtct cgaacagtac cgagttcacg ttgaagtgac ccggaatgta
     7201 gaagtacggc aggttccagt agcgacccac gtcgatagtg atcgacaagc cacccagtga
     7261 ataaccaaac agactcgcca acagcgccgg acgcaccagc ggatggtatt gcccacggtt
     7321 aaagacgtat actgcccacg ccagcgccca gccgccacag gcaaagccgg taccgatcag
     7381 caggtcaaac gcgatccaca cgccccacgg gaagccgccg ttcaggtcgg agacagagcc
     7441 cagaccgaac accagacgct tcacaatcag gagcatacag atgacgatta acggtccaaa
     7501 aatcatgacc ggtttactga tgattttgcc gcccagcggt tgtggatcat gactcatgat
     7561 cgtctcctcc gtcgtgatgg tcgtttttag tgttgcgacg aaccagcacg gttaagcccg
     7621 ccagcacagc cagtggtagc atcatgcctt tatacagggt gtgttggaca tgttcggaac
     7681 gcgcaccagt agaaagatcg tccagtttcg gcaggtcgag attttcataa ggcacacccg
     7741 tcagtaccag aacctgagta ccgccgccct ctttctcgcc gtacagatgc ggataatatt
     7801 tcggcaccgt atgcaggtaa gtgtcgccag atttcagcgt ctgacgcgga tagtggtatt
     7861 cgctgcccgg cttcagcgcc agacgttttt tcgcctccgc catcagctct tcacgcgtac
     7921 caaaaatcac cgcgcccgcc gggcacactt ctacacagcc aggcagaccg cctttatcga
     7981 gacgttccac acctttctgg ttgcacaact cgcacttatg cagcgcacca aacgggttgt
     8041 tgtagtcgta cttcggcacg ttgtatgggc aggcgaccat gcagtaacgg caaccggtgc
     8101 acacatcttt gtcgtaatgg acaataccgg ttttcggatc ttttttcagt gcagagaccg
     8161 gacacacaga gacacagttc ggatcgacgc agtgcataca ctgtttctta atgtacgcgt
     8221 agccattctc ctcctggtct ttgttgaccc ctgtgccgct ggtccacacc tgaatgatgt
     8281 tattggtata cggcgacagt ttgtcgttgt tcgaccaggt ctgttcccct tgcgggttac
     8341 gttcagggaa gttgatatcc tgacacttgg tgacgcaagc ctgacagcct acgcacaagg
     8401 tcgagtcgta caacatcccc agcgatcccg gaattggcgg gcggttttca gcagccgcat
     8461 gactgacaga cggcagcgcg cccgtcagca atgccccgca ggaggctgct ttaataaaat
     8521 tacgtctgtt cacggttatt ctccccgtga gtcagcgtta tctttctttt gctgacgacc
     8581 cagttcacgc accgccatca cgctgacacc ggcaaccagc ccaaccacac cgccgagcaa
     8641 accaatagcg cctgccgaga cgttgccgcc ttctttagcg ttaacgtccg gtttctgtga
     8701 acgcggagtc tggttttcga cgttggcaag ctgatggatg cctttatgga agccgatacc
     8761 ttcttcgtta cagccatagc acgggtgacc aatcgctacc ggccacacgc cgccaacatc
     8821 gcagaattgc agcgttgagc agttgccgta agtttctggc cctttacagc cgaggtggta
     8881 caggcaccag ccttcgcggt ggccttcatc accgaactct ttggcaaaac gaccagcatc
     8941 gaagtgcggg cgacgttcac agtgttcgtg aatcagacga ccataggcga aagtcggacg
     9001 gtttttgtca tccagtttcg gcggtttgcc gtaagtgatg atgtgcgcaa cggtcgccag
     9061 gaagttgtgc gggttcggcg ggcagccagg aatattgata acggttttgc ccggtagaac
     9121 ttcttgcagg ctgactgcgc cagttgggtt aactccagct gcggcaacac cgccccacgc
     9181 agagcaggaa ccgatagcga taatggctgc tgcgccttcc gccgctttgc ggatgtgatc
     9241 cacaatcggc tcaccggcaa ccatgcaata aataccgtta tcttttaatg ggatggaacc
     9301 atccaccact aacacatact gccctttgta cttctcgaga gcgttatgtt tgttctcttc
     9361 gacctgatga ccgaaggcgg cggaaagcac ttcgtgatac tccagagaga tagtctccag
     9421 cacgaggttt tctaccgttg gatgcgttgc acgaagcaga gattccgtac aaccggtgca
     9481 ctcctgcgcg ccaatccaga taactggcgg acgctgcggg ttagtaaccg attcgaccat
     9541 ctctgcagcg gctttgctac ttaaccccat ggtggcggct aatgctgcac aaagcttcat
     9601 gaaatcacga cggttaatgc cgtgagaatg gatgagggtg ttatctccag tcatttatag
     9661 ttattccgtt gcgaagacct ggcatatatt ttgcctcaat cgcaaaatca ataatgcgat
     9721 cgatgcgcca tttaccacac atttattatg gttatcgata tcatgatact gcggcggggc
     9781 actaaaacga agggaagaag cattagtgta attaattaaa gcaagataat acgtatgttt
     9841 gatcaatttt ctaggcgata accggccata aaaaagcgca acgtagaaca ggaattattc
     9901 tcacgaaaga aatccattac catctcttta tccagtccat aacggcgcgt cagaataccc
     9961 gcttcccagg cgctaagctg gcgatctccg gtcttttcgt acatccggtg tgagtagcct
    10021 aatacaaaac cgcgtttata atcggcacag aattttgtca cattaaccgc actgtcggca
    10081 tgcgttgctt ttaatcccgc cattaagcct ttaccaaaat ggttattcat cgttcacctc
    10141 attcgctata tattgtcata tatagcgata ttttaggcaa agaacgacat ttgcgcacgc
    10201 tattttttca tgcttgttta agacgtaatc ctgtaagaga taaacccatc aggagagata
    10261 tttatcgaac catttcagcg tcctttgcca ggcaagatcg gcggcagatt tgtcataacg
    10321 gggcgtggaa tcattatgga atccgtgatt aacccccgga tatatatacg cctcatagac
    10381 cttattattg gctttcaacg ccgcctcgta agcgggccag ccctcgttga ttcggctatc
    10441 cagttcagcg tagtggagca gtaaaggcgc ttcaatcttc gccacatcgg cagtgggtgc
    10501 ctgacgccca taaaacggca ccgcgcaggc cagttccgga tacgcgactg ctgccgcgtt
    10561 cgatacgccc ccgccatagc aaaatccgct aatacccact ttgcctgtcg cttgcggata
    10621 gcgttgcata aactcaattg cggcaaagaa atcattcatc agtttggttg gatcaacctg
    10681 ctgttgcagt tcgcggcctt tatcatcgtt accaggatag ccacctactg agcttaaacc
    10741 gtctggcgcc agggcgatgt acccttcctt cgccactcgc cgcgtcacat cttcgatata
    10801 cggattcagc cctcgattct catgcaccac taccacggct ggcgttttac cgctcatttt
    10861 tgcgggcttc accagataac cccgtacctc gccgtgacca tttggcgagg gatacgtgat
    10921 gtactctgca acaatttccg ggtcggtaaa ctctacctga gtcgccagcg cataatttgg
    10981 cttgagcaaa tcaaacagcg ctaatgccgt catcccgccc accgcatact tcgccgcaag
    11041 gttgaggaac tcacgtttcg agattttccc gtgagcgtaa tagtcgtagt aatccagcaa
    11101 ctcttgtggg aaagctttgg cagttaaacg cggcatcgtt gcactcctca actggtgttt
    11161 ttttaagcaa agcataagcg cgtgtttttg cccagttttt cgtcactctg tgagccagac
    11221 tacgggatac gcgctggcga atcgctaaac tagaaacatt gtttcgaaat tgaacggtgg
    11281 aaaggagagg tcatggtctg gttagcgaat cccgaacgtt acgggcagat gcagtaccgc
    11341 tattgcggaa aaagtggttt acgcctaccc gcgttatcgc tcggtttatg gcacaatttc
    11401 ggtcacgtta acgcgctgga atcacagcgt gcgatcctgc gtaaagcgtt tgatttaggc
    11461 attacgcact ttgatttagc caacaattac gggccgcctc caggaagcgc agaagagaac
    11521 tttggtcgcc tgctgcgcga ggattttgcc gcttatcgcg atgaactgat tatctctacc
    11581 aaagccggat acgatatgtg gcccggccca tacggctctg gcggctcacg taaatacctg
    11641 ctcgccagcc tcgaccaaag cctgaagcgt atggggcttg agtatgtcga tatcttttac
    11701 tctcatcgcg tcgatgaaaa tacgccgatg gaagaaaccg cctctgcgct ggctcatgcg
    11761 gtacaaagcg gtaaggcgct gtatgtcggg atctcctctt actcgccaga gcggacgcaa
    11821 aaaatggtcg agttgctgcg cgagtggaaa attccgctgt taattcatca accttcgtac
    11881 aatttactga accgctgggt ggataaaagc ggcctgctgg ataccctgca aaataacggc
    11941 gtgggctgca ttgcctttac tccactggct cagggattgc tgaccggaaa atatctcaac
    12001 ggtattccgc aagattcacg gatgcatcgt gaagggaata aagttcgtgg tctgacgccg
    12061 aaaatgctca ccgaagccaa cctcaacagc ctgcgcttat tgcatgaaat ggcacagcag
    12121 cgtggacaat caatggcgca aatggcgtta agctggttgc tgaaagatga gcgagtgacg
    12181 tcggtattga ttggtgccag ccgcgcggag caacttgagg agaacgtgca ggcgctgaat
    12241 aatctgacat ttagcaccga ggagctggcg cagattgatc agcatatcgc cgatggagag
    12301 ctgaatctgt ggcaggcgtc ttccgataaa tgacctgtta ataacgggcg cgagaaccgc
    12361 gcccgaatca gatcagtgat tatgacgagt cagtcggtca agatagccca tcacaaatgc
    12421 agagagcaca aacgtcagat ggataatgac gtaccacatc agtttgttat cagggacatt
    12481 tttcgcatcc ataaagacgc gcagtaagtg aatggaagaa attgccacaa tcgacgctgc
    12541 tactttgttt ttcagcgacg ttgcgtccat tttccccagc cagttcagct tctctttgtt
    12601 ctcagagata tcgagttgtg agacgaaatt ctcgtaaccg gaaaacatca ccatcaccag
    12661 caaaccgcca accagcgtca tatccaccag cgacagcaac acgaggatca aatctgattc
    12721 cgccatcgag aagatattcg gcagtacgtg aataatctcc tggaagaact tcagcgccag
    12781 ggcaactaac gccagcgaaa ggccaaagta cacgggggca agcagccagc gagaagcata
    12841 cattgcattt tcaagaaaac gttccataca ttcctgtcgt gtagttaaaa cgggcaacag
    12901 tatatcgcaa ttaagtaaac agcacgcaac agcaaatcaa catatacgtt aaacctttgt
    12961 cgcataattc tgagggttag ggatcttcct ggttccctta tcaacgcatc gagagcgtct
    13021 acccttaaag gacgttgaac ctgaagggag aaaaacgatg tctcatttaa aagacccgac
    13081 cacgcagtat tacactggtg aatatcccaa acagaaacaa ccgacgccag gcatccaggc
    13141 gaagatgaca ccggtaccgg attgcggcga gaaaacctat gttggtagcg gtcgcctgaa
    13201 agatcgtaaa gcactggtga cagggggcga ttccggaatc ggtcgcgctg ccgccattgc
    13261 ttacgcgcgt gaaggggctg acgtggcgat cagttatctt cccgtggaag aagaagacgc
    13321 tcaggatgtg aaaaagatca ttgaagaatg cggacgcaaa gccgttctgc tgccaggcga
    13381 tttaagcgat gagaaattcg cccgttcgct ggttcacgaa gcacacaagg cgttaggcgg
    13441 gctggatatt atggcgctgg tagccgggaa acaggttgcc attcctgata ttgcagacct
    13501 caccagcgaa cagtttcaaa agacctttgc cattaacgtt ttcgcgttgt tctggctaac
    13561 ccaggaagcg atccccctgc taccgaaagg tgcaagtatc atcaccactt cctcaatcca
    13621 ggcataccag ccaagcccgc atttactgga ctatgcggct acgaaggcgg caattctgaa
    13681 ctacagccgt ggcctggcaa aacaggtcgc ggagaaaggt attcgggtga atattgtcgc
    13741 gccaggcccg atctggacag cactgcaaat ttctggcgga caaacgcagg ataagatccc
    13801 gcagtttggt cagaaaacgc cgatgaagcg tgcggggcaa ccggcggagc tggcccctgt
    13861 atatgtttac ctggcaagtc aggagtcgag ctacgtcacc gcagaagtgc acggtgtctg
    13921 cggcggcgag catttaggtt aaaaaaatgc ccggttgtga aaagcaaccg ggcatcattt
    13981 ttatttcgct ttcgcagttt cttcgccgac cagacctatt ttcaggtaac ccgcctgatg
    14041 cagcgtatcc attaccttca tcaacgtctc gtaatcgaca gttttatccg cacggaagaa
    14101 gatggtggtg tctttcttgc cttcggttaa cgcattcaac gccgtaatca ttgtttcatc
    14161 ggtgaccgga tcgttaccaa taaacatcga gttgtctgct ttcaccgaca gataaaccgg
    14221 tttttccggg cgcggctgcg gcgtgctggt agaagcaggc aaattcacct tcacatctac
    14281 cgtcgctaac ggtgccgcca ccataaagat aatcagcaaa accaacatca cgtcgataaa
    14341 cggtgtcacg ttgatatcat gcatttcgcc gttatcgtcg aggttttcgt taagatgcat
    14401 cgccatcgga cattatcctg cacgtaattt ttgtgcgaca cgaaccggat gcgcagcggc
    14461 gctggcttcc agatccaggt cacggctttg cagcaacaat acctgcgctg caacatcacc
    14521 cagcatcgct ttaaagccgc caatctggcg tgcaaatacg ttatagataa cgaccgcagg
    14581 aatcgctgcc acgaggccga ttgccgttgc taacagagct tctgcaatac ccggcgcaac
    14641 gactgccagg ttagtggtct gcgtttgcgc gataccaata aagctgttca tgatgcccca
    14701 gaccgtacca aacagaccaa cgaacggcga aatcgcgccg atggttgcca gatagccgtt
    14761 accacggccc atttgacgac ctactgcggc gacccgacgc tccagacgga agctggtacg
    14821 ttctttaata ccttcgttat cgtcgctgcc ttctgacagt tccagctcat tctgcgcttc
    14881 attgagcaaa tgcaggctca agcttttgct accaaaatca gcggcgatat cgttggcctg
    14941 gtttaaggaa cgcgcttcag ccagcagttg ctgctcgcgc ttaagacgac gcttctgatt
    15001 gaagaactcc acgctcttac tgaagaagat tgcccaggtg actacggagg ccaaaataag
    15061 cccaatcatc acgcacttaa cgacaatatc ggcgtgctga tacatacccc agacggagag
    15121 gtccgtctgc attaaattat tacccactgt gtatctccag gacgcaagtc acaaaatctg
    15181 tgcataataa tatcaaaacg acgtcgaatt gatagtcgtt ctcattacta tttgcatact
    15241 gccgtacctt tgctttcttt tccttgcgtt tacgcagtaa aaaagtaacc agcacgccat
    15301 ttgcgaaaat tttctgcttt atgccaattc ttcaggatgc gcccgcgaat attcatgcta
    15361 gtttagacat ccagacgtat aaaaacagga atcccgacat ggcggacaaa aagcttgata
    15421 ctcaactggt gaatgcagga cgcagcaaaa aatacacgct cggcgcggta aatagcgtga
    15481 ttcagcgcgc gtcttcgctg gtctttgact gtgtggaagc caaaaaacac gcgacacgta
    15541 atcgcgctaa tggcgagttg ttctatggac ggcgtggaac gttaacccat ttctccttac
    15601 aacaagcgat gtgtgaactg gaaggtggcg caggctgtgt gctatttccc tgcggggcgg
    15661 cagcggttgc taattccatt cttgcttttg tcgaacaggg cgatcatgtg ttgatgacca
    15721 acaccgccta tgaaccgagt caggatttct gtagcaaaat cctcagcaaa ctgggcgtaa
    15781 cgacatcgtg gtttgatccg ctgattggtg ccgatatcgt taagcatctg cagccaaaca
    15841 ctaaaatcgt gtttctggaa tcgccaggtt ccatcaccat ggaagtccac gatgttccgg
    15901 cgattgttgc cgccgtacgc agtgtggtcc cggatgccat cattatgatc gacaacacct
    15961 gggcagccgg tgtgctgttt aaggcgctgg gttttggcat tgatgtttcc attcaggccg
    16021 ctaccaaata tctggtgggg cattcagatg cgatgattgg cactgccgtg tgcaatgccc
    16081 gttgctggga gcagctgcgg gagaatgcct atctgatggg ccagatggtc gatgccgata
    16141 ccgcctatat aaccagccgt ggcctgcgca cattaggtgt gcgtttgcgc caacatcatg
    16201 aaagcagtct gaaagtggct gaatggctgg cagaacatcc ccaagtagcg cgagttaacc
    16261 accctgctct gcctggcagt aaaggccacg aattctggaa acgagacttt acaggcagca
    16321 gcgggctatt ttcctttgtg cttaagaaaa aactcaatga tgaagagctg gcgaactatc
    16381 tggataactt cagtttattc agcatggcct actcgtgggg cgggtatgaa tcgttgatcc
    16441 tggcaaatca accagaacat atcgccgcca ttcgcccaca aggcgagatc gattttagcg
    16501 ggaccttgat tcgcctgcat attggtctgg aagatgtcga cgatctgatt gccgatctgg
    16561 acgccggttt tgcgcgaatt gtataacatt gccacttttg gacaattttg cagacatttt
    16621 attgtgaaaa gtcttaaatt gttgcgtccg ggatcaaggc gtcccggacg attcaggagt
    16681 acaataggca gataaaggct taaacgctgt tccacaggaa agtccatggc tgttattcaa
    16741 gatatcatcg ctgcgctctg gcaacacgac tttgctgcgc tggcggatcc tcatattgtt
    16801 agcgttgttt actttgtcat gtttgccacg ctgtttctag aaaacggctt gctgcccgcc
    16861 tcatttttgc caggcgacag cttgttgata ttggctggcg cattgattgc ccagggggtt
    16921 atggattttc tgcctacgat tgcgattctg accgccgcag caagtctggg ctgctggcta
    16981 agttatattc aggggcgctg gttagggaat accaaaacgg tgaaaggctg gctggcacag
    17041 cttcctgcta aatatcacca gcgcgccacc tgcatgtttg accgccacgg tctgctggcg
    17101 ctgctggctg gacgttttct tgcatttgtc cgtacgctgc tgccaaccat ggcgggaatt
    17161 tccggtctgc caaaccgccg cttccagttt ttcaactggt taagcggatt gctgtgggtc
    17221 agcgtggtaa ccagtttcgg ctattcctta agtatgattc cgttcgttaa acgacatgaa
    17281 gatcaggtaa tgacgttcct gatgatcctg ccaattgcct tgttaaccgc tggcttgttg
    17341 ggcacgctgt ttgtggtgat taaaaaaaaa tactgtaacg cctgacgatt ttccccgttc
    17401 ccggttgctg taccgggaac gtatttaatt cccctgcatc gcccgcattc ttgccgcatc
    17461 ttcccccggc gtcacaccga agtaacgttt aaactcacgg ctaaattgcg atgcgctttc
    17521 atagccgacg cgcatcgccg ccgcgctggc cttcatgccg tcgtgaatga tcatcatccg
    17581 cgccttatgc agacggtaat tcttcaaata ctgcaacggc gaggtgctgg tgacagactt
    17641 aaaattatgg tggaacgccg atacgctcat gttggcttct gccgccagtt gctcgacgct
    17701 caggttttcg gtgtatttat tctcaatccg tttcagcacg cgactaatca ggctgaagtg
    17761 agtctggcgg ctgaccagtg ccagtaacgc gccgccgcaa ggtccggtca gcacgtagta
    17821 cagaatttcg cggataatct gtttgccgag aatgcgcgca tccagcggtc gctccatcac
    17881 atccagtaac cgctctgccg cgcataaaat ctcttctgat aacgtggcgg agttaatacc
    17941 gctggctgcc attgacggct gaaaatgttc atcttcgcca atgtccatca acagttcctg
    18001 taactgcaaa atatcgacat tgagacgcaa ccctgccaac ggcacctctg acgtggcata
    18061 ggtttcgcac tcaaacggca acggcaccgt cagcaggaga tattcattag cgtcataacg
    18121 aaacacgcgt tcattgatat aaccgatttt atgtccggaa aagagaatta tgatgccagg
    18181 ctcgtacatc accggtgtgc gtgcgaaagg cgtctcgcca tacaacaaac gcacatcggg
    18241 caacagctct gacaaactat tttctttatt tttcagttta ttaactttat ccgccagcaa
    18301 gcggcaaatc tcttcacgtt tcatatcgcg taatttctta ggaataatgc ggcaatttga
    18361 ttgtgcgcaa ttttgtagca tttctccagc actctggaga aataggcaag acattggcag
    18421 aaatgagcat tgagaggtac ggcgctggcg accacaatga aaaacatcag gcagaccgat
    18481 ctctgccctc atattgaccc agcaaaggga gcaagtaatg aacaacttta atctgcacac
    18541 cccaacccgc attctgtttg gtaaaggcgc aatcgctggt ttacgcgaac aaattcctca
    18601 cgatgctcgc gtattgatta cctacggcgg cggcagcgtg aaaaaaaccg gcgttctcga
    18661 tcaagttctg gatgccctga aaggcatgga cgtgctggaa tttggcggta ttgaaccaaa
    18721 cccggcttat gaaacgctga tgaacgccgt gaaactggtt cgcgaacaaa aagtgacttt
    18781 tctgctggcg gttggcggcg gttctgtact ggacggcacc aaatttatcg ccgcagcggc
    18841 taactatccg gaaaatatcg atccgtggca cattctgcaa acgggcggca aagagattaa
    18901 aagcgccatc ccgatgggct gtgtgctgac gctgccagca accggttcag aatccaacgc
    18961 aggcgcggtg atctcccgta aaaccacagg cgacaagcag gcgttccatt ctgcccatgt
    19021 tcagcccgta tttgccgtgc tcgatccggt ttatacctac accctgccgt cgcgtcaggt
    19081 ggctaacggc gtagtggacg cctttgtaca caccgtggaa cagtatgtta ccaaaccggt
    19141 tgatgccaaa attcaggacc gtttcgcaga aggcattttg ctgacgctga tcgaagatgg
    19201 tccgaaagcc ctgaaagagc cagaaaacta cgatgtgcgc gccaacgtca tgtgggcggc
    19261 gactcaggcg ctgaacggtt tgatcggcgc tggcgtaccg caggactggg caacgcatat
    19321 gctgggccac gaactgactg cgatgcacgg tctggatcac gcgcaaacac tggctatcgt
    19381 cctgcctgca ctgtggaatg aaaaacgcga taccaagcgc gctaagctgc tgcaatatgc
    19441 tgaacgcgtc tggaacatca ctgaaggttc agacgatgag cgtattgacg ccgcgattgc
    19501 cgcaacccgc aatttctttg agcaattagg cgtgccgacc cacctctccg actacggtct
    19561 ggacggcagc tccatcccgg ctttgctgaa aaaactggaa gagcacggca tgacccaact
    19621 gggcgaaaat catgacatta cgctggatgt cagccgccgt atatacgaag ccgcccgcta
    19681 agctttttac gcctcaaact ttcgttttcg ggcatttcgt ccagacttaa gttcacaaca
    19741 cctcaccgga gcctgctccg gtgagttcat ataaaggagg aacgtatggc taatccaacc
    19801 gttattaagc tacaggatgg caatgtcatg ccccagctgg gactgggcgt ctggcaagca
    19861 agtaatgagg aagtaatcac cgccattcaa aaagcgttag aagtgggtta tcgctcgatt
    19921 gataccgccg cagcctacaa gaacgaagaa ggtgtcggca aagccctgaa aaatgcctca
    19981 gtcaacagag aagaactgtt catcaccact aagctgtgga acgacgacca caagcgcccc
    20041 cgcgaagccc tgctcgacag cctgaaaaaa ctccagcttg attatatcga cctctattta
    20101 atgcactggc ccgttcccgc tatcgaccat tatgtcgaag catggaaagg catgatcgaa
    20161 ttgcaaaaag agggattaat caaaagcatc ggcgtgtgca acttccagat ccatcacctg
    20221 caacgcctga ttgatgaaac tggcgtgacg cctgtgataa accagatcga acttcacccg
    20281 ctgatgcaac aacgccagct acatgcctgg aacgcgacac acaaaatcca gaccgaatcc
    20341 tggagcccat tagcgcaagg agggaaaggc gttttcgatc agaaagtcat tcgcgatctg
    20401 gcagataaat acggcaaaac ccctgcgcag attgttatcc gctggcatct ggatagcggc
    20461 ctggtggtga tcccaaaatc ggtcacacct tcacgcattg tcgaaaactt tgatgtctgg
    20521 gatttccgtc tcgacaaaga cgaacttggc gaaattgcaa aactcgatca gggcaagcgt
    20581 ctcggtcccg atcctgacca gttcggcggc taacgtgcaa attctcccgg tggcggtaat
    20641 gttccgctac cggacttttc agaaatcatt tattcccctc gcgtcccgcc cgttgctact
    20701 cttccttgtt caggaatgcc aaatataagg acatcatcat gcagagccgg aagctcttaa
    20761 aagaacaact catctatatc cgggataaac gcaacggaga ggtgaaaaac agatgaaaat
    20821 aatacttctg tttttagcag ccctggcaag ttttaccgta cacgcacagc ccccctcaca
    20881 gaccgtagaa caaacagtcc ggcatattta tcagaactat aaatcagatg ccactgcccc
    20941 ttattttggt gaaaccggag agcgagcgat aacttctgcg cgtattcaac aggcgcttac
    21001 cctgaacgac aatcttacgc tgccgggcaa tattggctgg ctggattatg atccggtttg
    21061 tgattgtcag gattttggcg atctggtgtt agaaagcgta gcgataaccc aaactgacgc
    21121 cgatcatgcc gatgccgttg tgcgctttcg tatctttaaa gacgataaag aaaagacctc
    21181 gcagacactg aaaatggtgg cggaaaatgg tcgttgggtc attggcgata ttgtcagcaa
    21241 tcatggcagc gtcttacaag cggttaatag cgagaatgaa aaaacgctgg ccgctatagc
    21301 ttcgttgcaa aaagaacagc cggaagcctt tgttgccgaa ctctttgaac atattgctga
    21361 ttatagctgg ccgtggacgt gggtggtttc cgactcttac cgccaggcgg tcaatgcctt
    21421 ctataaaacc accttcaaga cggccaacaa tcccgatgaa gatatgcaaa tagaacggca
    21481 atttatttac tacaatccga tctgttttgg cgaagagtcg ttattttcac gcgttgatga
    21541 aattcgagtc ctggagaaaa ccaccgattc cgcccgcatt catgttcgct ttacgctgac
    21601 caatggcaac aacgaagagc aagaactgat tttacagcgg cgcgaaggta agtgggaaat
    21661 cgctgatttt atccgcccga acagcggcag cctacttaag cagattgagg caaaaaccgc
    21721 cgccagatta aaacaatgag ctgaattaaa taacaattag ccggaacaat aaataaaagg
    21781 gaacactata tgaaaacgat tttcaccgtg ggagctgttg ttctggcaac ctgcttgctc
    21841 agtggctgcg tcaatgagca aaagatcaat cagctggcga gcaatgtgca aacattaaat
    21901 gccaaaatcg cccggcttga gcaggatatg aaagcattac gcccacaaat ctatgctgcc
    21961 aaatccgaag ctaacagagc caatacgcgt cttgatgcac aggactattt cgactgcctg
    22021 cgctgcttgc gtatgtacgc agaatgataa aaaaatcccc ggcagcattc tgttaccggg
    22081 gatatttttt aacgtccaac cgccgcttta gggcgtttct tcgcaccagc attctgcagg
    22141 cgagattgcg tagacgacgc tttttttgcc gtagcaggcg tctgacgctg ggtcgccatc
    22201 ggcgtatgtt tagtcaacgc cggacgggta ttgcggttct gacggcgagc ttcacgcatc
    22261 tcttcaatgg ttggcgcagg cactaagcaa tcgcgacggc tgccaatcag atgctttttg
    22321 cccatcgctt ccagcgcctg gcggattaac ggccagtttg ccggatcgtg gtaacgcaac
    22381 aacgctttat gcaggcgacg ctgcttgttg cccttcggta cgaagacgtc ttcactctta
    22441 taaccaatct tcgccagcgg gtttttgccg gtgtaataca tggtggtcga gttagccagc
    22501 ggcgacggat agaagttctg cacctggtcg aggcggaagc gatgcttttt cagccatagc
    22561 gccagattca ccatatcttc atcacgcgta cccgggtgcg cggagatgaa atacgggatc
    22621 agatactgtt ctttacctgc ctgtttcgag taagtatcga acagctcttt aaagcggtca
    22681 tagctgccca tgcccggctt catcatcttc gataacggcc cttcttcggt atgttccggg
    22741 gcaatcttca gataaccgcc aacgtgatgg gtcgccagct ctttgatata gcgcggatct
    22801 tctacggcga tgtcataacg cacaccagag gcaatcagga tctttttaat gcctttcaga
    22861 tcgcgcgcac ggcgatagag gttaatcgtc ggttcgtggt tagtgtccat atgcggacaa
    22921 atatccggat aaacgcacga caaacgacgg caggtttgct cagcgcgtgg cgatttgcaa
    22981 cgcaacatat acatgttggc agtaggccca ccgagatcgg aaatcacgcc cgtaaaacct
    23041 ggaacagtgt cgcggatcgc ttcgatctca ttaataatcg aatcttcgga acggctctga
    23101 ataatgcgcc cttcgtgctc ggtaatagaa cagaaagagc agccgccaaa gcagccacgc
    23161 ataatgttga ccgagaaacg aatcatttcg taagccggaa ttcgggcatt accgtaagcc
    23221 ggatgcggca cgcgcttgta cggcagcgca aagacgctat ccatctcttc ggtagaaagc
    23281 ggaatagcag gcgggttgat ccatacatag cggtcgccat gtttttgcat caatgcgcgg
    23341 gcgcagcctg ggttagtttc gtggtgcaga atgcgcgaag catgggcgta cagcacttta
    23401 tcgcccttca ctttctcgaa agaaggcagc aacacgtagg ttttttccca cggtttcggg
    23461 cgtggtggct gcacggttac agctttggct tcctgctttt tcggagctac cggtttgtta
    23521 tccgcgcacg gcaaatcttc gccatacgga tgcgggattg ggtcgatttt ccctggggta
    23581 tcaagacggg tggaatccac accgctccag ccaggcaacg cctcttttac gataatcgcg
    23641 gtattacgca catcgcggat ttcactaatc ggctcgccca tcgccagacg gtgcgccact
    23701 tccaccagcg gacgctcgcc gttaccgaac atcagcatgt cagctttgga atccaccaac
    23761 accgaacggc gcacggtatc cgaccagtaa tcataatgcg cggtacggcg caggctggcc
    23821 tcaataccgc cgaggatcac cggcacatct ttccacgcct ctttacaacg ctgggtataa
    23881 accagtgtgg cgcgatccgg acgcttaccc gcgacgttat ccggtgtgta ggcatcgtca
    23941 tgacgtaaac ggcgatcggc ggtatagcgg ttgatcatcg agtccatgtt gccagcagta
    24001 acaccgaaaa acagattcgg tttacccaga cgcataaagt cgtctttact gctccagtct
    24061 ggctgggcga tgatcccgac gcgaaagccc tgcgcttcca gcatacgacc gcaaatcgcc
    24121 atcccgaagc ttgggtgatc gacatacgcg tcgccagtaa ccaaaatgat gtcgcagcta
    24181 tcctagccaa gttgatccat ctcttcacgt gacatcggca aaaacggtgc cggtccaaaa
    24241 caggcggccc agtactgcgg ccaggagaac aggtcgcgat ccggttggat cagggagata
    24301 gagctcattt tgcttccaga aatggtaaaa aaataatcaa aggccgggga ttatacgctg
    24361 tatcacgcgc ggaaatgaag tgatgttgat gaacggtggc gaaataccct ggtgaggttt
    24421 ttcactgccc gacttgggca agcacattta aggctggcgg tatcgtggca actgccgcta
    24481 ctatttatgc cgatcagcaa cgttcaaatc atattgcacg acgatcggct atccgaggac
    24541 acccacagta aggcaaggta tggaacaggt tattacaaag cgccgctatt acgatattgg
    24601 tctacagatt gaagagctgc tgtattcagg cgtattcaaa gccggagaac gcttaccttc
    24661 agaacgtgaa ctgagcgagc ggtttaatac cagccgaact accattcgtg aagccattat
    24721 tatgctggaa ctgaaaggcg tattgaatgt gaagcaaggg tcagggattt tctttgtcga
    24781 ctccaccgat aagctcaacc agaaatcatt aatgccctat tcagaaattg gcccgtttga
    24841 actgttgcag gcgcgtcagg tgattgagag caatattact ggttttgcag ccagccagat
    24901 tagctttaac gagttacaag agttaaagaa aatcattgga ttacaggaaa aagccatcgc
    24961 tgcggaaagt gataaatttg aagacctgga tcaccgattt catagcatta ttgccgaagc
    25021 gacgcaaaat cgggtactca tcaaacaagc cgctgaatta tggcgggcag tgcgcacaga
    25081 aaacccccgc tggaagaaac ttaactacaa gtacctgcat gaaaagcatt tgcgtctgca
    25141 atggctggaa gatcaccggg ctattttcct ggctctgcaa cagaaagatt cagagctcgc
    25201 cagggaagcg tcctggagac atctggaaaa cagcaaaaat gaactgatca aaatcttcaa
    25261 acaagacgct tccatcagcg attttgatga ctttttcttc gctcgttaac cttcattagt
    25321 ccagagctga cgtgaaaaat gacgctggct ctgcgttgca ccctccactg gtatacgctc
    25381 ccttctcaat tggtctaata aatcgattat catcagatat cttagattag cggcaattat
    25441 cccaaaaact gacctctaaa aaactttcct ttcattttat tcatataact cattgataca
    25501 gatataaata acgccttgtt gttctgtttc gtacttttgc ctttctctgc aagcgtgatg
    25561 catctctctg tttttggtct agtgaattat gtaaattggt ctatacacac cagctaccag
    25621 gagtaagagc ataatgtcag ggaacgaaag tatcgatgcc gcccactatg atcaggtaac
    25681 cacctggcct cgggacgagt tacaggcaga tattgtccat attggttttg gcgcttttca
    25741 tcgcggacac caggctgtct acacggatct cactaatcag ctttcggaca cccactgggg
    25801 gatctttgag atcaacctgt ttggtgatgc tcaactgatc gaaaacttaa atgcgcaaaa
    25861 tgggctgttt tcggttgtgg aaacatctgc atcgcaatct acctcacgcc ttgtgcgttc
    25921 ggtaactggc ggtattcata cccccgtgga tggcattgcc gcagcaatcc agaaattgac
    25981 tgaacctcag gtaaaaatcg tttcattaac catcaccgag aaaggctatt gtctcgattc
    26041 gcaaacgcga tcgcttgatc tcaccaatgg attaatcaaa cacgatctac aaaacccgga
    26101 tgcgcctcag tcggctattg gcttgatcgt ctgtgctttg caacaacgta aagcggcagg
    26161 gctcgccgct tttagcgtgc tctcctgtga caacttgcca gacaatgggc atctgacgcg
    26221 caatgccgtt ctcggttttg cccgacaact ggaccagccc ctggctcagt ggatcgaaga
    26281 aaatgtctca tttccaggca cgatggtcga tcgcattgtt ccggcaatga ctgaatcgca
    26341 attcgcctta ctggaaacta aaaccggtta tgccgatccc tgcgggatcg tctgcgaatc
    26401 atttcgtcag tgggtgatcg aagataattt tgtgcgcgga cgaccggaat gggataaagc
    26461 cagcgcgatg tttgtcagca atgttcagcc ttatgaagag atgaagttac gcatgttaaa
    26521 tggtagccat tcatttctgg cttataacgg ctcactggct ggctatgagt ttatctggca
    26581 atgcatggaa gacgctaatt ttcgttccat tacccgccaa ctgatgatta atgaacaagc
    26641 ccgaacactt aatccagact taaatatcaa catccaggaa tacgccgacc tgttaattga
    26701 acgctttagc aaccgtaatg ttgcacaccg taccgggcaa atagctatgg acggttcgca
    26761 aaagcttccc cagcgagcgc tgacgccctg gctgaaattg catcagcaaa aacaaaacaa
    26821 tgctgttctg tcactgctcg ttgctggttg gttgcattat gtcattgatg ctgttgagaa
    26881 aagccagtct gtcgctgatc caatgaatga ccaacttcag gcgctaataa aggaacaaca
    26941 agacgcatgg caacaggcgc tcgcattact gcaccttagc gccatatttg gtgatttaag
    27001 caaccatcag ccatttataa atgaaataaa aatcgccttt gcaaatataa aaaacaaagg
    27061 catcaaggcc accatcagcc aattattatc ggatgagcag aaatgaaaac attaatttgt
    27121 cagcagcctg gcgttatgga atatgtggaa aaggatattc ccacaccagc agataatgaa
    27181 gtgctgttaa aaatcaaagc tgtgggt
//
LOCUS       DAZGJT010000063        27050 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZGJT010000063 DAZGJT010000000
VERSION     DAZGJT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.063"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(235..1608)
                     /gene="argH"
                     /locus_tag="ACHKFK_002906"
     CDS             complement(235..1608)
                     /gene="argH"
                     /locus_tag="ACHKFK_002906"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO9053197.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKALEDLPLSELQKFSQVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(1669..2445)
                     /gene="argB"
                     /locus_tag="ACHKFK_002907"
     CDS             complement(1669..2445)
                     /gene="argB"
                     /locus_tag="ACHKFK_002907"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO9053198.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(2453..3457)
                     /gene="argC"
                     /locus_tag="ACHKFK_002908"
     CDS             complement(2453..3457)
                     /gene="argC"
                     /locus_tag="ACHKFK_002908"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO9053199.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDVAFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKPGVSQVQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3611..4762
                     /gene="argE"
                     /locus_tag="ACHKFK_002909"
     CDS             3611..4762
                     /gene="argE"
                     /locus_tag="ACHKFK_002909"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO9053200.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLAKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYDAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            5064..7715
                     /gene="ppc"
                     /locus_tag="ACHKFK_002910"
     CDS             5064..7715
                     /gene="ppc"
                     /locus_tag="ACHKFK_002910"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO9053201.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKSGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7898..9631
                     /locus_tag="ACHKFK_002911"
     CDS             7898..9631
                     /locus_tag="ACHKFK_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO9053202.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPEDQGKFDGNTEHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAT
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9857..10708
                     /locus_tag="ACHKFK_002912"
     CDS             9857..10708
                     /locus_tag="ACHKFK_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO9053203.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFIDTGAGAALVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQSQEQQTARLIVTSLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10695..11036)
                     /locus_tag="ACHKFK_002913"
     CDS             complement(10695..11036)
                     /locus_tag="ACHKFK_002913"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9053204.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(11038..11916)
                     /locus_tag="ACHKFK_002914"
     CDS             complement(11038..11916)
                     /locus_tag="ACHKFK_002914"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO9053205.1"
                     /translation="MTSSAGQRISCNVVEMRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDAAQARDVVKMNLQRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(11882..14179)
                     /locus_tag="ACHKFK_002915"
     CDS             complement(11882..14179)
                     /locus_tag="ACHKFK_002915"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO9053206.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVIMRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQIHILRYAELAETMAASCT
                     DGQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMIFDQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14230..14550)
                     /locus_tag="ACHKFK_002916"
     CDS             complement(14230..14550)
                     /locus_tag="ACHKFK_002916"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO9053207.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14565..15644)
                     /locus_tag="ACHKFK_002917"
     CDS             complement(14565..15644)
                     /locus_tag="ACHKFK_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO9053208.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGVMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTEEKEDDLDLDFEIN"
     gene            15953..18454
                     /gene="ptsP"
                     /locus_tag="ACHKFK_002918"
     CDS             15953..18454
                     /gene="ptsP"
                     /locus_tag="ACHKFK_002918"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO9053209.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPISLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIAALTPWQHQTIYIDG
                     NAGAIVVEPGESVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIPLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRQLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            18466..19128
                     /gene="fsa"
                     /locus_tag="ACHKFK_002919"
     CDS             18466..19128
                     /gene="fsa"
                     /locus_tag="ACHKFK_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9053210.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVKEAKHLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            19139..20242
                     /gene="gldA"
                     /locus_tag="ACHKFK_002920"
     CDS             19139..20242
                     /gene="gldA"
                     /locus_tag="ACHKFK_002920"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO9053211.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            20518..21135
                     /locus_tag="ACHKFK_002921"
     CDS             20518..21135
                     /locus_tag="ACHKFK_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO9053212.1"
                     /translation="MKASLALFSLLTLFTSYSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYVQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     AYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPTSKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFAK"
     gene            complement(21162..22067)
                     /gene="yijE"
                     /locus_tag="ACHKFK_002922"
     CDS             complement(21162..22067)
                     /gene="yijE"
                     /locus_tag="ACHKFK_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO9053213.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLTILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKRI"
     gene            complement(22161..24341)
                     /gene="katG"
                     /locus_tag="ACHKFK_002923"
     CDS             complement(22161..24341)
                     /gene="katG"
                     /locus_tag="ACHKFK_002923"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO9053214.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGGGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24670..25560)
                     /gene="metF"
                     /locus_tag="ACHKFK_002924"
     CDS             complement(24670..25560)
                     /gene="metF"
                     /locus_tag="ACHKFK_002924"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO9053215.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPAELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLRRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25942..>27050)
                     /locus_tag="ACHKFK_002925"
     CDS             complement(25942..>27050)
                     /locus_tag="ACHKFK_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9053216.1"
                     /translation="TIIEKDGVKIGVIGLHGVFAFNDTVSAATRVGIEARDEIKWLQR
                     YIDELKGKVDLTVALIHEGVPARQSSMGGTDVRRALDKDIQTASQVKGLDILITGHAH
                     VGTPEPIKVGNTLILSTDSGGIDVGKLVLDYKEKPHNFTVKNFELKTIYADEWKPDQQ
                     TKQVIDGWNKKLDEVVQQTVAQSPVELKRAYGESASLGNLAADALLAAAGKNTQLALT
                     NSGGIRNEIPAGAITMGGVISTFPFPNELVTMELTGKQLRSLMEHGASLSNGVLQVSK
                     GLEMKYDSSKPVGQRVITLTLNGKPIEDATVYHIATQSFLADGGDGFTAFTEGKARNT
                     TGGYYVYHAVVDYFKAGNTITDEQLNGMRVKDIK"
BASE COUNT         6502 a         7501 c         6824 g         6223 t
ORIGIN      
        1 tgtattggct gatgtattga aaatataatt tttatgggga gagtgaaggt aaagaagacg
       61 caagaataaa aaggaatgtg acaaataatg gttctggttg ggcgttaaat ttaaggtcac
      121 cagaatactg acaaatttct gcctgatgcg gcgtaaacgc cttatccgcc tacgaagtgt
      181 cacactttgt aggcgtggta agctccccat tttcagggga gcaatataaa caaattaccc
      241 caaccgcgcc tgcgcaaaag caatcgcctg cgccacctgt tgcggtgaga caccgccttt
      301 tgctgcacgc ttgtcgaggc acgattgcag cgacagaatc ggatagacat cttcgccaat
      361 cacctggctg aatttctgca actcactgag cggcagatct tccagcgctt tgccctgacg
      421 aatggcttcc accaccgctt cacccacaat atggtgcgcc tcgcggaacg gtacgccttt
      481 cgccaccaga taatccgcca gttcagtagc gttggcgtaa ccctgctgcg ccgcttcctg
      541 gcaacgcgga cgtttcacct gaatgccgtc cagcaccagc gccgccatat gcaggcagtc
      601 cagccaggta tcgagcgcgt cgaacagacc ttctttgtct tcctgcatat ctttgttgta
      661 agccagcggc aaacctttca gcgtcatcat catgccggtt aacgccccct gcacccgacc
      721 gcatttaccg cgaatcagct ccagcgcatc cgggtttttc ttctgcggca ttaatgatga
      781 accggaagtc acacggtcag aaagctccac aaaccccgct tcgccggtgt taaagaaaat
      841 cagatcttca gcaaaacgcg acagatgcac catgccgata gcggcagcag aaagcagttc
      901 caacacatgg tcacggtcag aaacgctgtc gagactgtta cgggtcgccg aagcaaagcc
      961 cagccagcct gctaactgtt cacggtcgat ttcataggcc gttcctgcca gtgcgccaca
     1021 gcctagcggg ctgacatcca gacgcttaag cgcatcctgc aaacggcttt catcacgcgc
     1081 cagcatctca acgtaggcca ggcaccagtg cgcgaacgtc accggctggg cgcgttgcag
     1141 gtgggtgtaa cccggcatca ccgcgtcctg attgttctgc gcggtctcga ccagagcgga
     1201 ttgtaactgc cggttagccg tcagcaactc gctaacggta tctttgcacc acagtttcag
     1261 gtcagttgct acctgatcat tacggctacg cccggtatgc agttttttgc ctaactgacc
     1321 cactttgtcg atcagtttgc cttccaccca gctatggata tcttcggcgt cgctttcaag
     1381 gatttgttgt ggcctggcgc gaacatcttc cagcagcacg ttcagcgcct cttccagttg
     1441 cgcctgctct tctgcggtta acacgccgac cgttaccagg gctttggacc aggccacaga
     1501 gccaacaata tcctgctccg ccagacggta atcaaagcgc agtgagtcgt tgaattgttt
     1561 gaaccgttga tctgctgcct gggtaaaacg cccgccccaa agtgccataa ctctgtttcc
     1621 ttattttgaa attcaatgcc ggaaagctgc gcctccggcc aacaaaactt aagctaaaat
     1681 ccgcgtaccc atcggcatac cgttaaacag tgccggaagc tgttccgcat gacgccagga
     1741 ggcgatatct accggacggc ccaacgtgcg cgcagcatcc agcgccgcgt tcactttcac
     1801 aatcatgccg tcagtaataa tgccctgctc aatcagttgt tctgctttcg cggcggtcat
     1861 ttcggcaata cgttgccctt tgccgtcgag aatgccgctg acgtcagaga gcaaaatcag
     1921 atccgcgccc agcgttgccg ccagcgccgt cgccgcctgg tcggcattga cattcatcag
     1981 ttgcccttcg tctgttacgc caatggagct gaccaccggc agataaccgt tctccagcaa
     2041 actgttgata agcttaggcg aacctggctg cgccagtcca acatggccta actcttcatc
     2101 aagctgggtc actttgacgc tgtcgccgtc accgagaaac aaacctacgg ccgcaatctg
     2161 atgtttcttc gcccatgcca gcagggtttt atttgccgtt cccgccagtg ctccggtgat
     2221 aatgtctatc tgatccgcag gcgtcacccg caggccattt ttctttttca ccggcagatt
     2281 cagccctttc atcagctcat ccaccacgca accgccgccg tgcacaatca ccagcggacg
     2341 ctgatgtgac tcgcgataat tcaccagtgc gctaaacaga cgttccagcg cctcttcact
     2401 atccagcagt acgccaccca gtttgataat taatggattc atcattgcac ccttaaataa
     2461 gagactgcgt ttccgcatag ccaaaacgaa tattggcgca ctgcaccgct tgtgccgccg
     2521 cgcctttcag taagttgtct tcggtcgcca caataatcag atgctcgccc tgaacggcaa
     2581 aaccgatatc gcagaatggc agtccaacga catttttcag cgccggaacg cctttgtcat
     2641 acagccgcac cagtggttta tgggcatacg cctgctgtaa cgcttgcgcc acttgcacct
     2701 ggctcacacc cggtttcagg cggcaggtaa tggtttcgag aatgccgcgc gggaaattgc
     2761 ccagatgcgg ggtgaagata acgtcagcac cgaggtgtgt ggcgatctct ggttgatggc
     2821 gatgggtaaa gacgccatac ggttgcaggc taacttcaca aaaactattt gaaatcgctg
     2881 ccttacgtcc tgcaccgctc acgccgctgg tggcgttgat caccggccac tgattgaggt
     2941 caagaagatc ggcatcaatc aacggtttca gcgccagctg tgctgccgtc ggataacagc
     3001 ctggcacggc aatcaagttc gcttctttta atttattacc gcaccactcc gccagaccgt
     3061 aggctgcctg ttccagcagt tccgggtatt gatgggtaaa gccgtaatat ttttcataga
     3121 aggcgacatc gttaacacga aacgcgccgg aaaggtcgaa caccacacag cctgcttcaa
     3181 gaaattgcgg cgctaaatcg tggctgactt catgggcggt ggcaagaaac accacgtcca
     3241 cccctgggct aaactcgctg atatccgaca tcggctgcaa cggcagatca acgatgcctt
     3301 ttagctgcgg atgcaaatcg gagattaact ttcccgcatc attgctttgc gctgaaacag
     3361 tcaaagcggt tatgttcata tgcggatggc gatttacata ggtcactagc tctgcgccag
     3421 cgtagccgct ggcacccaca atcagcgtat tcaacatcgg ggctattcac cttcttatgt
     3481 ctggttgcca ggttaaacgt aaaacattca ccttacggct ggtgggtttt attacgctca
     3541 acgttagtgt atttttattc ataaatactg catgaatatt gatactatca tgaccagagg
     3601 tgtgtcaaca atgaaaaaca aattaccgcc atttatcgag atttaccgtg ctctgattgc
     3661 cacaccttca ataagcgcca cggaagaggc actcgatcaa agcaatgcag atttaatcac
     3721 tctgctggcg gactggttta aggatttagg cttcaatgtg gaagtacagc ctgtgccagg
     3781 aactcgcaac aaatttaata tgctggccag ttgcggacag ggggctggcg gtttgttgct
     3841 ggcggggcat accgatacgg tgccatttga tgacggacgc tggacgcgcg atccatttac
     3901 gttgacggag catgacggca agctttacgg cttaggcacc gccgacatga aaggcttttt
     3961 tgcgtttatc cttgatgcgc tacgcgatgt cgacgtcacg aagcttgcaa aaccgctcta
     4021 cattctggcg actgccgatg aagaaaccag tatggccgga gcgcgttatt ttgccgaaac
     4081 taccgccctg cgcccggatt gcgccatcat tggcgagcca acgtcgctgc aaccggtacg
     4141 cgcgcataaa ggtcatatct ctaacgccat ccgtattcag ggccagtcgg gccactccag
     4201 cgatccagca cgcggagtta acgctatcga actgatgcac gacgccatag ggcatatttt
     4261 gcaattgcgc gataacctga aagaacgtta tcactacgac gcgtttaccg tgccataccc
     4321 tacgctcaac ctcgggcata tccacggtgg cgacgcttct aaccgtattt gtgcttgctg
     4381 tgagttgcat atggatattc gtccgctgcc aggcatgaca ctcaatgaac ttaatggttt
     4441 gctcaacgat gcattggctc cagtgagcga gcgctggccg ggtcgtctga cggtcgacga
     4501 gctgcatccg ccgatccctg gctatgaatg cccaccgaat catcaactgg ttgaagtggt
     4561 tgagaaattg ctcggagcaa agaccgaagt ggtgaattac tgtaccgaag cgccgtttat
     4621 tcaaacgtta tgcccgacgc tggttctggg gcctggctca attaatcagg ctcatcagcc
     4681 tgatgaatat ctggagacga ggtttatcaa gcccacccgc gaactgatca cccaggtgat
     4741 tcaccatttt tgctggcatt aaaacgtagg ccaaacaatg tcggatgcga tgcttgcgca
     4801 tcttatccga cctacacctt tggtgttact cgaggcgatt ttttaacatt cccataagtt
     4861 acgcttattt aaagcgtcat gaatttaatg gcgtaaattc ctgctattta ttcgtttgct
     4921 gaagcaattt cgcagcattt gacgtcaccg cttttacgtg gctttataaa agacgacgaa
     4981 aagcaaagcc cgagcatatt cgcgccaatg cgacgtgaag gatacagggc tatcaaacga
     5041 taagatgggg tgtctggggt aatatgaacg aacaatattc cgcattgcgt agtaatgtca
     5101 gtatgctcgg caaagtgctg ggagaaacca tcaaggatgc gttgggagaa cacattcttg
     5161 aacgcgtaga aactatccgt aagttgtcca aatcttcacg cgctggcaat gatgctaacc
     5221 gccaggagtt gctcaccacc ttacaaaatt tgtcgaacga cgagctgctg cccgttgcgc
     5281 gtgcgtttag tcagtttctg aacctggcca acaccgccga gcaataccac agcatttcgc
     5341 cgaaaggcga agctgccagc aacccggaag tgatcgcccg caccctgcgc aaactgaaaa
     5401 accagccgga actgagcgaa gacaccataa aaaaagcagt ggaatcgctg tcgctggaac
     5461 tggtcctcac ggctcaccca accgaaatta cccgtcgtac actgatccac aaaatggtgg
     5521 aagtgaacgc ctgcttaaaa cagctcgata acaaagatat cgccgactac gaacataacc
     5581 agctgatgcg ccgcctgcgc cagttgatcg cccagtcatg gcataccgat gaaatccgta
     5641 aactgcgtcc aagcccggta gatgaagcca aatggggctt tgccgtagta gaaaacagcc
     5701 tgtggcaagg cgtaccgaat tacctgcgcg aactgaacga acaactggaa gagaatctcg
     5761 gctacaaact gcccgtcgaa tttgttccgg tccgttttac ctcgtggatg ggcggtgacc
     5821 gcgacggcaa cccgaacgtc actgccgata tcacccgcca cgtcctgctg ctcagtcgct
     5881 ggaaagccac cgatctgttc ctgaaagata ttcaggtgct ggtttctgaa ctgtcgatgg
     5941 ttgaagcgac ccctgaactg ctggcgctgg ttggcgaaga aggtgccgca gaaccgtatc
     6001 gctatctgat gaaaaacctg cgttctcgcc tgatggcgac acaggcatgg ctggaagcgc
     6061 gcctgaaagg cgaagaactg ccaaaaccag aaggcctgct gacacaaaac gaagaactgt
     6121 gggaaccgct ctacgcttgc taccagtcac ttcaggcgtg tggcatgggt attatcgcca
     6181 acggcgatct gctcgacacc ctgcgccgcg tgaaatgttt cggcgtaccg ctggtccgta
     6241 ttgatatccg tcaggagagc acccgccata ccgaagcgct gggcgagctg acccgctacc
     6301 tcggtatcgg cgactacgag agctggtcag aagccgacaa acaggcgttc ctgatccgcg
     6361 aactgaactc caaacgtccg cttctaccgc gcaactggca accaagcgcc gaaacgcgcg
     6421 aagtgctcga tacctgccag gtgattgccg aagcgccgca aggctccatt gccgcctacg
     6481 tgatctcgat ggcgaaaacg ccgtccgacg tgctggctgt ccacctgctg ctgaaagaag
     6541 cgggtatcgg gtttgcgatg ccggttgctc cgctgtttga aaccctcgat gacctgaaca
     6601 acgccaacga tgtcatgacc cagctgctga atatcgactg gtatcgcggc ctgattcagg
     6661 gcaaacagat ggtgatgatt ggctattccg actcagcaaa agatgcgggc gtgatggcag
     6721 cttcctgggc gcaataccag gcacaggatg cattaatcaa aacctgcgaa aaagcgggta
     6781 ttgagctaac gttgttccac ggtcgcggcg gttccattgg tcgcggcggc gcacctgccc
     6841 atgcggcgct gctgtcacaa ccaccaggaa gcctgaaaag cggcctgcgc gtgaccgaac
     6901 agggcgagat gatccgcttt aaatatggtc tgccagaaat caccgtcagc agcctgtcgc
     6961 tgtacaccgg ggcgattctg gaagccaacc tgctgccacc gcctgagccg aaagagagct
     7021 ggcgtcgcat tatggatgaa ctgtcagtca tctcctgcga tctctaccgt ggctacgtac
     7081 gtgaaaacaa agattttgtg ccttacttcc gctccgctac gccggaacaa gaattgggta
     7141 aactgccgtt gggttcacgt ccggcgaaac gtcgcccaac cggcggcgtc gagtcactgc
     7201 gcgctattcc gtggattttt gcctggacac aaaaccgcct gatgctcccc gcctggctgg
     7261 gcgcgggtac ggcgctacaa aaagtggtgg aggatggtaa acagagcgag ctggaagcca
     7321 tgtgccgcga ttggccattc ttctcgacgc gtctcggcat gctggagatg gtcttcgcca
     7381 aagcagacct gtggctggcg gaatactatg accaacgcct ggtagacaaa gcactgtggc
     7441 cgttaggtaa agagttacgc aatctgcaag aagaagacat caaagtggtg ctggcgattg
     7501 ccaacgattc tcacctgatg gccgatctgc cgtggattgc agagtctatt cagctacgga
     7561 atatttacac cgacccgctg aacgtattgc aggccgagtt gctgcaccgc tcccgccagg
     7621 cagaaaaaga aggccaggaa ccggatcctc gcgtcgagca ggcgttaatg gtcactattg
     7681 ccgggattgc ggcaggtatg cgtaataccg gctaatcttc ctttcctgca aaccctcgtg
     7741 cttttgcgcg agggttttct gaaatacttc tgttctaaca ccctcgtttt caatatattt
     7801 ctgtctgcat tttattcaaa atctggatat cccttcagat atccttaagg aattgtcgtt
     7861 acattcggcg atattttttt caagacaggt tcttactatg cattccacag aagtccaggc
     7921 taaacccctt tttagctgga aagccctggg ttgggcactg ctctactttt ggtttttctc
     7981 tactctgcta caggccatta tttacatcag tggttatagt ggcactaacg gcattcgcga
     8041 ctcgctgtta ttcagttcgc tgtggttgat cccggtattc ctctttccga agcggattaa
     8101 aattattgcc gcagtgatcg gcgtggtgct atgggcggcc tctctggcgg cgctgtgcta
     8161 ttacgtcatc tacggtcagg agttctcgca gagcgttctg tttgtgatgt tcgaaaccaa
     8221 caccaacgaa gccagcgagt atttaagcca gtatttcagc ctgaaaattg tgcttatcgc
     8281 gctggcctat acggcggtgg cagttctgct gtggacacgc ctgcgcccgg tctatattcc
     8341 aaagccgtgg cgttatgttg tctcttttgc cctgctttat ggcttgattc tgcatccgat
     8401 cgccatgaat acgtttatca agaacaagcc gtttgagaaa acgttggata acctggcatc
     8461 gcgtatggag cctgccgcac cgtggcaatt cctgaccggc tattatcagt atcgtcagca
     8521 actaaactcg ctaacaaagc tactgaatga aaataatgcc ttgccgccgc tggctaattt
     8581 caaagatgaa tcgggtaacg aaccgcgcac cttagtgctg gtaattggcg aatcgaccca
     8641 gcgtggacgc atgagtctgt acggttatcc gcgtgaaacc acgccggagc tggatgcgct
     8701 gcataaaacc gatccgaatc tgaccgtgtt taataacgtg gttacgtctc gtccgtacac
     8761 cattgaaatc ctgcaacagg cgctgacctt tgccaatgaa aagaacccgg atctgtatct
     8821 gacgcagccg tcgctgatga acatgatgaa acaggcgggt tataaaacct tctggatcac
     8881 caaccagcag acgatgaccg cccgcaatac catgctgacg gtattttcgc gccagaccga
     8941 taagcagtac tacatgaacc agcaacgtac gcagagtgcg cgtgaatacg acaccaacgt
     9001 gctgaagccg ttccaggaag tgctgaagga ccctgcgccg aagaaactga tcatcgttca
     9061 tctgctgggt acgcatatca agtacaagta ccgttatccg gaagatcagg gcaaatttga
     9121 cggtaataca gagcatgttc caccgggatt aaacgcagaa gagctggagt catataatga
     9181 ttatgacaat gctaacctgt acaacgatca cgtggttgcc agcctgatta aagactttaa
     9241 agcgaccgat ccgaacggtt tcctggttta tttctctgac cacggtgaag aggtttacga
     9301 cacgccgccg cacaaaactc aggggcgtaa tgaagacaac ccgacgcgtc atatgtacac
     9361 cattccgttc ctgctgtgga cgtcagaaaa atggcaagcg actcatccgc gtgatttctc
     9421 acaggatgtt gatcgtaaat acagcctggc ggaactgatc cacacctggt cagatttggc
     9481 gggcttatct tacgacggtt acgatccaac ccgttcagtg gtgaatccgc agttcaaaga
     9541 aactacccgc tggattggta acccgtataa gaaaaacgca ctgatcgatt acgacactct
     9601 gccgtatggc gatcaggtgg gtaatcagta attatcagcc agatgttatt tgccggatgc
     9661 gacgcttgac gcgtcttatc cggcctacag atcgcggcgg taggccggat aaggcgttta
     9721 cgccgcatcc ggcaatacaa gtcgactgaa actgccgcaa tgtttacatc tcactcccat
     9781 caggccacac ttttcaggtg gccttttttc ataatgccag ttactgtttt cccgtctaca
     9841 tacctaaggg aataacatgt atcacgacgt cagctatctg ctctcccgcc tgatcaacgg
     9901 cccgctgtcc ctgcgccaga tttactttgc cagcagcaac ggcccggcgc ccgatcttgc
     9961 gtatcaggtc gattttccac ggctggaaat tgtgctggaa ggtgagttta ttgataccgg
    10021 cgctggagca gcgttagttc ccggcgatgt gctgtacgtc cctgctggtg gctggaattt
    10081 tccacaatgg caagcccccg ccaccacctt tagcgtgctg tttggcaaac agcaactcgg
    10141 cttcagcgtt gtgcaatggg atggcaaaca atatcaaaat ctggcgaagc aacacgtcgc
    10201 ccgacgcggc ccgcgcattg gttcttttct gctacaaacg ctcaatgaaa tgcaaatgca
    10261 gtcgcaggag cagcaaacgg ccagacttat cgtcaccagc ctgcttagcc actgccgcga
    10321 tttgctcggc agccaaatac agaccgcctc acgcagccag gctctattcg aagctattcg
    10381 tgattatatc gacgaacgct atgcctccgc acttacccgc gaatctgtcg cacaggcgtt
    10441 ctatatttcg ccaaattacc tctcgcacct gtttcaaaaa acgggggcca ttggttttaa
    10501 cgaatacctg aatcacacgc gactggagca cgctaagaca ttattgaaag gctatgacct
    10561 gaaggtaaaa gaagtggcgc acgcctgcgg ctttgtcgac agcaactact tctgccgtct
    10621 gtttcgtaaa aataccgaac gctcgccgtc ggagtatcgt cggcagtacc acagccaact
    10681 gacagaaaaa ccgactactc cagaataaga tgggtttgct gcggcgcaga aagcactttg
    10741 cgcactgcgc tcatcaccct ctgcggctca cgcaggaaag cgtagatgct gcattgcacg
    10801 tagcgacaat gttcaaatcg ctcagcaccg gcaagctcga tatccgtaat taacagcgca
    10861 acatccgccc ggcggatatc ctcgtccgct aaacgattct ccgttcccaa cgcgccctga
    10921 gtttcaatgc tgacgcccca cttctctaac tggcacaact tttccagccg ttccgccgcc
    10981 atataagtat gcgccacgcc gcttacgcag gcggttactg ccaccagata tgccatttta
    11041 acctcccacg gtaacctgaa atccggcccg ttctgccatt tcacgcatcg ttgccacatc
    11101 ggctgacgac ggcgcagcaa cctctttcat cgaccatgtt ttccccagca ggcggtattt
    11161 cggttcgccg tactgatgaa acggtaacag atggatctgc ctgatattca gcgggatcag
    11221 cacgtccagc gcctgctgca tattctcccg gctgagcgtg aaaccaggga tcaacggtaa
    11281 acgcgggatc acgttgacac cctcactcac cagcaaacgc agattctcca gcacgcgttg
    11341 caggttcatc ttcaccacat cccgagcctg agccgcatcc ataattttta aatcgaacaa
    11401 cacttcatcg cacaatttcg ccagcggtaa cagcttgctg gctggcgcgt ctccggcagt
    11461 ttcgatggcg catgacacac cccatagccg cagtcgctgt aaaaaacggg tcgcaaactc
    11521 cgcctgcatt aacacttcgc cgccagaaag cgtgacgcca ccgccggacg tgcgaaaaaa
    11581 aatgtcatct ttcatcactt cccgttccag cgcgtcaagg ctgatatcgc ggccaatccg
    11641 ttcaaatgcc ccggaggggc attcatccgc atcacgcaaa catttcgcgc agtgcagaca
    11701 tttcgcctct ctgcgcaccg tctggatttt gccggagatc gactccggat tagcacacca
    11761 tgggcaaaga tgcggacagc ctttaaaaaa gaccaccgta cgaatgccct cgccgtcatt
    11821 cagtgaataa cgctgaatgt tgaaaatgcg cgccacatca tcgcggcgca tttccacaac
    11881 gttacagctg atgcgctgtc cggcggatga tgtcatcctg gatctccttc gacaattcga
    11941 caaagaaggc gctgtatccg gcaacgcgca ccaccagccc ggcataatct tgtgggcgct
    12001 gttgcgcttc ccgtaacgtg tcggcgttca ccacgttaaa ctgaatatgc tgcaacttaa
    12061 gctgggtaaa tgcccgtaag aagtcagcca gtttgcgtaa tcccgcttca ccttccaggg
    12121 tcgccggagt gaatttcacg ttcagcaacg taccgttaga cagcagcgta ttatcgagct
    12181 tactgactga tttcagcacc gccgttggcc cttgtgcatc ctgacccagc atgggtgaca
    12241 agccaccgtc tgccagttgt tccccggcaa aacgaccgtc tggcgtcgcg ccaaccaccg
    12301 atcccaacgg gacgtgagca gaaacggtat acgatcctgg cgtgaagtag ccgccgcgcg
    12361 ggttctggta tttttccact tctttgcagt agtggcgtaa cagttcggcg ctaatgttat
    12421 ccacctcgtc gatatcgtta ccgtatttct cgaagcggtt aattaagcga gcgcggactt
    12481 tttcgccttc tggcgtggcg aagttggctt ttaataccga cagcaattcg tcaaaactta
    12541 aacgctgttg atcaaaaatc atccctttca acgcatggag agaatcgctc aggttggcga
    12601 taccgatccc ctgtacgccg gagaagttat aacgcgcgcc gccatcggta atatcgcgac
    12661 ctttttccag acaatcgctg ataaaagacg agagtagcgg tacaggagcc cagtcgcgat
    12721 ggccgatatc acaaatattg ctgccctcaa ccatcagggt gatgtagtgg ctgatcttgg
    12781 cgcggatctg ttccagtaaa ccttcataag tcagcgcagc gttgccttca ttttcatgca
    12841 ggcagatttc catcactttc agcaggttaa acatcgcgat gtcatgcaag ccgtaggttc
    12901 tgccgggaat agataattcc acacagccca ctacggaata gtcgcgcgca tcttccagcg
    12961 aaacgccacg gttgaggaac gctggcacca ccacttcatc gttaaagatt tgcggaatac
    13021 cggtgccgag gcgaatggtt tctgcggttt tcatcaggaa cggcgtgtcg atcaaggcgt
    13081 tagtgcgcac gccgaggttc ggttgcggta attgcacgct ttgataagcg tcaaggcaaa
    13141 ggaacgaaag cacgttcacc gcgctacgtc cgttctcggt taacccgccg agcagtgcgg
    13201 tatagccggt agggaaacct gcgaaataac gcgcgctact ggtggagcgc aacagcacga
    13261 tgtcgttgca tttcacccat aaagattcga gcagttcttt caggaatgcc ggatcttcgc
    13321 cctgggttaa tgatgcctga tagaacggca acatatactg gtcgaagcgc cccaacgata
    13381 gcgaactggc gttggattcg tattgcagaa tgatgttcat gtaccagaat aactggcacg
    13441 cctgccagaa cgtctgcggc ttatgttcgg cgttatggcg ggagatctcc gcaatggtca
    13501 gcagttcttc gcgacgctgg ccatcagtgc agcttgccgc catcgtttcc gccagttcgg
    13561 cgtaacgcaa aatatgtatc tgcgaggctt ccagcagtaa cagcgctgcc tgataaaagt
    13621 gattctccgg ctgttgctga caatgttgct gcatctgtgc caccagctcc cccagcccgt
    13681 gattcagcag gcgcgggtaa tcaataataa tgtgcccctg gcctttatcc gtctggttga
    13741 tgctgaaaat ctgcgtgctg gtcgcggctt ttacttcatc cgtcatctgc ccgttgatga
    13801 aatctttcat cgaacgtttt tcccagtacg ggaacaactc ttcgcggtag atacgtttat
    13861 cttcttcgct gatagcaaag cggtcctgcg gacgcgtcgg gaattgatcc agctctttca
    13921 gcagccagta agggtccatt tccggcgaca taatcccggc gcgcggtttt acggtgcggt
    13981 taccggcaat cagttcttca tcacgaatcg aaatttcaac atgttcaagg atatacgctg
    14041 ttgctttcgc ccgacgcatt atcaccggct ccccttcggt ttgccgatgg ctggcggtat
    14101 aaagcaacgc ccgctccagc gagatttcac gggtattggc aaacagtgca gttttgaggc
    14161 gagagatacg attcgtcatg aggaactcct gtgggaaagc gagcccgccc ttgcgggcgg
    14221 gataagaact taagcggttt gtgccaggtg agcttcaatt ttgctcataa tggcgtcagc
    14281 acgctttacc gcgtcgctga tgttgacacg cacgatagtc ttgccagcaa aacgctcttc
    14341 aaatttgatg ccgatatctt tggtcaaaat caccatatcg gcgcttgcca cgtcttccgc
    14401 agtcagttca ttttccagac caatcgaccc ctgggtttct actttcactt cccagccttt
    14461 cgctttcgcg gcgctttcca gcgcctctgc cgccatataa gtatgggcaa cacctgaagg
    14521 acatgcggtt actgcgataa ttttcgtcat ggctcgttcc tcaattaatt aatttcaaaa
    14581 tccagatcca ggtcgtcttc cttctcttca gttgaagacc catttttacg cgccagactt
    14641 ttcagcacgt tcacgcagac tgccgtcaca accgccccga cagccactgc cgcgatgtaa
    14701 cccaacttac cttcaaccac cggcagcaca atcagaccac cccagcctgc atagcactgc
    14761 gcaccgacca gcgccgcagt taccgcacca caaacagaac cgaccatgat ggaaggaatg
    14821 acacgcaacg gatcggctgc ggcgaaagga atcgcccctt cagtaacgcc aacacagccc
    14881 atcaccagtg ccgccttgcc ggtttcgcgc tcttctgcgg agaaattttt acgaccaatc
    14941 agcgtcgcca gccccatccc cagcggcggg atacaaatac ccacggcagc aatggccacc
    15001 acggtataaa caccctgagc aacgcaaatc agcatgaagg cataggccac tttgttaacc
    15061 gggccgccca tatcgaacgc cagcatcaga cccatgatca ccgccagcat aacaatgctg
    15121 ccctgctgca tcccctgaag ccactgggtc aggctgttgg tcaacgcccc caccggctcg
    15181 cccagccccc acatcatgac gccagcggta atcagtgtgc cgacgattgg aatgatgaag
    15241 attggcatca ccgagcgcag aactttatgc accggaattt tcttcaggta atgcaccacg
    15301 atgccgccga taatcccggc gatcagcgca ccaaagaagc ccgcgccaaa gctgttacca
    15361 acccaggcac cgatagcgca tggagccagc gcagaacgtt ctgcaatgga ataaccgatg
    15421 taagcggcga ggaaaggcac catcagcgtc aaacctgcaa cgccgatatc aaacagtttt
    15481 ttcagattcg gatcggctac ggcatccggc actgcgcctt tgccatacaa catgacggaa
    15541 accgccagca aaataccgcc cgataccacg aagggaatca tgtgtgaaac gcccgtcatt
    15601 aaatgctgac gggtattttt caggatctgc accaactcat tcataagctg ctcccgggct
    15661 tgcgaaagcc catgtccata tttttgatgg aagaaacaat aggcaatcgc aggcggagca
    15721 aacagtggat aaaaaggcca tttactggac ttttgtaata tcagtacaaa aatgcgatcc
    15781 gcctcataac ttgcgataat ccggaagtac gccgcaaaac ggcgtcttgt gagacgcgtc
    15841 gcaaattgac ggttacatta catttgtcca gcttttggca gatttgtcac atggcaggcc
    15901 gttgccgtgc gctcttatcc tgtaaacaga aaaccgtacc gggagaaagg caatggccct
    15961 gattgtggaa tttatttgtg agctacctaa cggcgtgcat gcgcgtccgg caagccacgt
    16021 tgaaacgctg tgtaatactt tttcatcaca aattgagtgg cataacctgc gcactgaccg
    16081 caagggcaac gccaaaagcg cccttgcgct gattggcacc gatacgctgg tgggcgataa
    16141 ctgccagtta ctgatttccg gggccgacga acaggaagcg caccagcgtt taagccaatg
    16201 gctgcgcgat gaattccccc actgcgacgc accgctggcg gaagtaaaat ctgacgaact
    16261 ggaaccactg ccgatttcac tgaccaatct gaatccgcaa attatccgtg ctcgcaccgt
    16321 gtgcagcggt agcgcaggcg gcattctgac gccgatctct tctttagatc tcaatgcgct
    16381 gggtaatctt cccgcagcca aaggcgttga cgccgaacaa tccgcactgg aaaacggtct
    16441 gacgctggta ctgaaaaaca tagagtttcg tctgctggat agcgacggtg ctaccagcgc
    16501 gattctggaa gcgcaccgat ccctggctgg cgatacttcc ctgcgcgaac atttactggc
    16561 aggtgtcagc gccggattaa gctgcgccga agcgattgtt gccagtgcga atcacttttg
    16621 cgaagagttc gcccgttcca gcagcagcta cctgcaagaa cgtgccctgg acgtacgcga
    16681 cgtctgcttc cagttactcc agcaaatcta cggtgagcaa cgcttcccgg caccgggcaa
    16741 actgacgcag cccgccattt gtatggctga tgaactgacc cccagccagt tcctcgaact
    16801 ggataaaaat catctcaaag gtctgttact gaaaagtggc ggcaccacat cacatacggt
    16861 gatccttgcc cgttcgttca acattccgac gctggtaggt gtggatattg ctgcccttac
    16921 tccgtggcag catcagacga tttatatcga cggcaacgca ggggcgattg tggttgagcc
    16981 aggggaatcc gtggcccgtt attatcagca agaagcccgc gtacaggacg ctctgcgtga
    17041 gcaacagcgt gtctggctga cccagcaagc gcgtaccgca gacggtatcc gcattgaaat
    17101 tgccgctaac atcgctcact ccgtggaagc gcaggccgca ttcggcaatg gtgcggaagg
    17161 cgttggtttg ttccgcactg aaatgctcta tatggatcgc accagtgcac cgggtgaaag
    17221 cgagctgtac aacatttttt gtcaggcgct ggagtctgcc aacggacgca gcattattgt
    17281 gcgcactatg gacattggcg gtgataaacc cgttgattat ctgaacattc cggcagaagc
    17341 taacccgttc ctcggttatc gcgcggtgcg tatttatgaa gaatacgcgt cgttgtttac
    17401 cacacaacta cggtcgatcc tgcgcgcctc tgctcacggc agcctgaaaa tcatgatccc
    17461 gatgatctcc tcaatggaag agatcttatg ggtgaaagaa aaactggcgg aagccaaaca
    17521 gcaactgcgc aatgaacaca ttccgtttga tgagaagatt ccactcggca ttatgctgga
    17581 agtaccatcg gtgatgttca ttatcgatca gtgttgtgaa gagattgatt tcttcagtat
    17641 cggcagtaat gacctgacgc aatatctact ggcagtggat cgcgataacg ctaaggtgac
    17701 tcgtcactac aacagcctga atccggcctt cttgcgggcg ctcgattacg ccgtgcaggc
    17761 ggtgcatcgc cagggcaaat ggattggtct gtgcggtgaa ctgggagcga aaggttccgt
    17821 gctgccgttg ctggtcggtt tagggctgga tgaactcagc atgagcgcac catcaattcc
    17881 ggcggcgaaa gcgcggatgg cgcaacttga tagccgtgag tgccgccagt tgctcaacca
    17941 ggcaatggcc tgccgtactt cgctggaagt ggaacacctg ctggcgcaat tccgcatgac
    18001 ccaacaggac gcaccgctgg tcaccgccga gtgcatcaca ctggaaagcg actggcgcag
    18061 caaagaagaa gtgctcaaag gcatgaccga taacctgctg ctggcgggcc gctgccgcta
    18121 tccgcgtaaa ctggaagccg acttgtgggc gcgcgaggcc gttttctcta ccggactggg
    18181 ctttagtttc gccattcccc acagtaaatc ggaacacatt gagcaatcca ccatcagcgt
    18241 agcgcgtctg caagcgccag tgcgctgggg cgatgatgaa gcgcaattca tcattatgtt
    18301 aaccctgaac aaacacgccg cgggcgatca acatatgcgc attttctcgc gcctcgctcg
    18361 tcgcatcatg cacgaagaat tccgtaatgc gctggttaac gccgcctctg ccgacgctat
    18421 cgccagcctg ctgcaacatg aactggaact gtaaaaggaa acatcatgga actgtatctg
    18481 gacaccgcta acgtcgcaga agtcgaacgt ctggcacgca tattcccgat tgccggggtg
    18541 acaactaacc cgagcattat cgctgccagc aaggagtcca tctgggaagt gctgccgcgc
    18601 cttcaaaaag cgatcggtga tgagggcatt ctgtttgctc agaccatgag ccgcgacgcg
    18661 cagggtatgg tgaaagaagc gaaacacctg cgcgacgcta ttccgggcat tgtggttaaa
    18721 attccggtaa cctctgaagg tctggctgca attaaaatgc tgaagaaaga aggcattact
    18781 acgctgggaa ccgcagtgta cagcgccgcg caaggattac tggcggcgct ggctggagcc
    18841 aaatacgttg ctccatacgt taaccgcgta gatgcccagg gcggtgacgg cattcgtact
    18901 gtacaggagc tgcaagcgct gttggaaatg catgcgccag aaagcatggt gctggctgcc
    18961 agctttaaaa caccacgtca ggcgctggat tgtttgctgg ctggatgtga atccatcaca
    19021 ctgcccttag atgtagcgca acaaatgctt aacacccctg cggtagagtc agctatagag
    19081 aagttcgagc acgactggaa tgccgcattt ggcactactc atctctaaag gagtaattat
    19141 ggaccgcatt attcaatcac caggtaaata catccagggc gcagatgtaa ttaatcgtct
    19201 gggcgagtac ctgaagccac tggcggaacg ctggttagtg gtgggtgaca aatttgtttt
    19261 aggttttgct caatccactg tcgagaaaag ctttaaagat gctggactgg tagtagaaat
    19321 tgcgccgttt ggcggtgaat gttcgcaaaa tgagatcgac cgtctgcgtg gcatcgcgga
    19381 gactgcgcag tgtggcgcaa ttctcggtat cggtggcgga aaaaccctcg atactgccaa
    19441 agcactggca catttcatgg gtgttccggt agcgatcgca ccgactatcg cttctaccga
    19501 cgcaccgtgc agcgcattgt ctgttatcta caccgatgag ggggagtttg accgctatct
    19561 gctgttgcca aataacccga atatggtcat tgtcgacacc aaaatcgtcg ctggcgcacc
    19621 tgcacgtctg ttagcggcgg gtatcggcga tgcgctggca acctggtttg aagcgcgtgc
    19681 ctgttcccgt agcggcgcga ccaccatggc gggtggcaag tgcactcagg cagcgctggc
    19741 actggctgaa ctgtgctaca acaccctgct ggaagaaggc gaaaaagcga tgcttgccgc
    19801 cgaacagcat gtagtgactc cggcgctgga gcgcgtgatt gaagcgaaca cctatttgag
    19861 tggcgttggt tttgaaagcg gcgggctggc ggcggcacac gcggtacata acggcctgac
    19921 cgctatcccg gacgcgcatc actattatca tggtgaaaaa gtggcattcg gtacgctgac
    19981 gcagctggtt ctggaaaacg cgccggtgga ggaaatcgaa accgtagctg cgcttagcca
    20041 tgcggtaggt ttgccaataa ctctcgctca actggatatt aaagaagatg tcccggcgaa
    20101 aatgcgaatt gtggcagaag cggcatgtgc agaaggtgaa accatccaca acatgcctgg
    20161 cggcgcgacg ccagatcagg tttacgccgc actgctggta gccgaccagt atggtcagcg
    20221 tttcctgcaa gagtgggaat aacctactcc aaactcccgg cttgtccggg agtttgaacg
    20281 caaaattgcc tgatgcgcta cgcttatcag gcctacgcaa tctctgcaat atagtaaatt
    20341 tacgtacttt tgtaggccgg ataaggcgtt cacgccgcat ccggcacgct gtgccagtaa
    20401 tctgaaactt ccaccttgtt cgggtgtttt tttatccgct tctggacatt ttctccacag
    20461 aacaatcgtt agcccggaag gtcgaatctg tgactcttat tttcacggac gaaacagatg
    20521 aaagcttcac tggcgttatt cagtcttctg acgttattta ccagttactc cttaaaatct
    20581 cccgccgttc caccaaccgt ggtacaaatc caggctaaca ccaatcttgc catcgcagac
    20641 ggcgccagac agcaaatcgg cagcacgcta ttttacgatc cggcgtatgt gcagcttacc
    20701 tatcctggcg gtgatgtccc gcaagaacgc ggcgtatgtt ccgatgtggt gatccgtgca
    20761 ttgcgcagcc agaaggtcga tttgcagaaa ctggtgcacg aagatatggc gaagaatttt
    20821 gctgcgtacc cgcaaaaatg gcagttaaag cgcccggaca gcaacatcga tcaccgtcgg
    20881 gtgcctaatc tggaaacctg gtttacccgc cacgataaaa cacgccccac cagcaagaac
    20941 cccagtgact atcaagcggg cgatattgtc tcctggcgat tagataacgg gctggcacat
    21001 attggggtgg tgtcagatgg cttcgcccgc gacggtacgc cgctggtgat acataacatt
    21061 ggcgcaggcg cgcaggaaga agatgtactg tttagctggc ggatggtggg gcactatcgg
    21121 tattttgcta aataagagat ctccccacat gaagaaaaaa ttcagatcct ttttacactg
    21181 acggcttctt ttttcttacg gctcaccagc gccagtgcca gtacaatcag cacaataccg
    21241 ctaccttcaa ctgcccccgg attctcaccg agcagccacc aggaaaagag tacgccgcaa
    21301 acgggaacgg ccagtgtgct taagctggca atactggcag gcaagttttt caatacaaac
    21361 aaccataagc tccacgccag cgctgtcgcc agaatcgcac tgtaggccag cgcccagaac
    21421 acggtgggtt gccagtcaat ttcacgttgc ggcaccagta aagcgaccac gctcatcacc
    21481 agcgccgcat acagcatctg ccaggatgtt agcgacaata aatccacgcg cggatggcgg
    21541 gcatacagac gtttagcaac aatcgcgctc gccccccaac tcacaccgga gaggattgtc
    21601 agcatggcac ttttcatcga agagaaatcg agttgccacg gctgcaacac caaaaataag
    21661 ccgaaagcgg caatcagtat cgcgaaatat tgcccacgtc gcaggcgttc accgagaaac
    21721 aacgcggcga aaatcaccac ccagaacggc atggtatagc tcaggattgc caccttcccc
    21781 gctccgccgc tgaccagcgc ccactgtgcc aggccaacca tcccgcaggt ttgtaacagg
    21841 gcaatggcta aggtgtattt aaacggcgtc gggcgcattc cgcgaccacg taataaaaga
    21901 acgatgaata aaacgagagc gccgaaaatg cagcgtaagg cggtgaagtc gaaggcaccg
    21961 atgtagctgg tgacttgctt catgaaaatc cagctataac tccagataag tgtgagcaca
    22021 accaggccac tgattgccag tgggttgctc tttcctgcgg cagacatgag agatccagtg
    22081 tgtagttttc ctgtggacac tatacgccgg taaagaaact tcagcgactg catgaaaggc
    22141 agtcgctgaa aaaggtcaga ttacagcagg tcgaaacggt cgaggttcat cactttcacc
    22201 cacgctgcca cgaagtcttt aacaaacttc tcgtgggcat cgctgctggc gtaaacttcc
    22261 gccaccgcac gcaggacaga gttagaacca aagaccagat ccgcacggct ggctgtgtat
    22321 ttcacttcgc cagtttcgcg gtcacggcct tcgaacagct ctttcgattc gtcggtcgct
    22381 ttccactcat aacgcatatc cagcaagttc acgaagaagt cattgctcaa tacgccaacg
    22441 cggtcagtga agacgccgtt tttgctgcca tcgaagttgg cacccagtac gcgcatcccg
    22501 cccaccagcg cagtcatttc cggcgcggtc agcgtcagtt gctgggcttt atcaatcaac
    22561 agtgactcgg tggtggaaac gtccagacga gcgcgatagt tacggaaacc gtcagcaatt
    22621 ggctccagca gttcgaacat ctcaatgtca gtctgatcct gacgcgcatc aacgcggccc
    22681 ggcgcaaacg gtacatgaat gctcaaacct gcggcgcttg cggctttctc aacgccaacc
    22741 acacctgcca gcacgatgat atccgccagc gaagctttgc cagactcttt ctgaattttc
    22801 tccagaactg gcagagcacg aacggccgcg gcgttcacat cccagtcacg ctgcggcatt
    22861 aatgccagac gcgcaccgtt ggcaccacca cgtttgtcgc caccacggaa ggtagaagca
    22921 gacgcccagg ccaccgatac cagctcacta acagacagac cagaatccgc aatcgcgaat
    22981 ttcagatcga taatgtcctg ctcggtcggg ttgtagatcg gctgcggcag cggatcttgc
    23041 cagatcagat cttctttcgg tacttccggt ccgatgtaac gagatttcgg ccccatatcc
    23101 ctgtgcgtca gtttgaacca ggcacgagca aaggcttcgt tgaacgcctg cggatcgttg
    23161 aggaaacgac gagagatctt ctcgaactca ggatcaaaac gcagcgtcag gtcggtcacc
    23221 agcatcgtcg gtttacgttt cttcgacgga tcaaacggat ccgggataat ttccggtgcg
    23281 tctaccgctt caaactggat tgcgccagcc gggctgcggg tctgtaccca ctcatacttg
    23341 aacaagttct cgaagaaata gttgctccac tgggtcggcg tctgggtcca gaccacttcc
    23401 agaccagagg taatggcatc tgcgccaacg ccgctgccgt aagtgctcgc ccagcctaaa
    23461 ccttgttctt caatcggtgc tgcttctgga tcaggaccta cgtttgatgt cggaccggca
    23521 ccgtgggttt tacccagcgt atgaccaccc gcaatcagcg ccacggtttc ttcgtcattc
    23581 atgcccatgt tgccgaaggt cgcgcggata gctgctgccg ccgaaagtgg ttcgccgctg
    23641 tgatccgggc cttccgggtt aacgtaaatc aggcccatct cggttgcacc cagcggtgct
    23701 ttcgccagcg cttccggatg acggtgagtc agccaggctt tttcatcacc ccagttaaca
    23761 tccagatccg gttcccagac gtcttcacga ccggcaccaa aaccgaaggt acggaagccg
    23821 gagttttcca gcgccacgtt acccgcgagg ataaacaggt cggcccagga gattttctga
    23881 ccatatttct gtttgattgg ccacaacagg cgacgcgctt tatcgaggct tacgttatcc
    23941 ggccaggagt tcagcggtgc aaaacgttgc tgaccacgac ccgcgccgcc gcgaccgtcg
    24001 attgaacggt aagtacccgc gccgtgccag gccatacgaa taaacagacc ggcgtagctg
    24061 ccccagtcgg ctggccacca cggttgagat tctgtcagca gggctttcag atcttttttc
    24121 aggccgtagt aatctaattt gctgaattct ttgcggtagt caaagtcctc acccagcggg
    24181 ttagaacgat tagaatgttg gtttaacagg tcaacacgga gttgatttgg ccaccagtcg
    24241 cgcgtggttg tgcccccacc tgcgctctgg tcgtgaccgc cctgatggaa cgggcatttg
    24301 ccggtggctg tcgtgttatg gatatcgtct gacgtgctca tcaatgtgct cccctctaca
    24361 gtgttaccgt tacgatacac agcgttagag agaagttata attgaattaa tccacggatg
    24421 cgatagttga gatcttacat attgttggtt aaagagatgt agatcaaatt gatcgtaatt
    24481 aagacttttt gattatgtcc gatttaggat aaatgcttta taaaaaaggg ttttagattt
    24541 gtatccttca agtaatgagg gagaaaacaa ggctgataac cttattttcc cgccctcatt
    24601 tcgaggcagc attttgtgct ctgtttaaaa tttgtgatca ctgtgtgatt ttcacaaaag
    24661 gcacactatt tataaaccag gtcgaactcc cagtgtatgg caaatcgcgt aactcatttc
    24721 agcgcggtta agcgtataga agtggaaatc tttcactcct tcacggctta aaatcttcac
    24781 catatccatg gcaatattcg cgcccaccag cttgcgggtt tcagcatcgt catccaggcc
    24841 gtcgaacatt tgtgccatcc acgccggaat acgcacgttg gtcatgtcgg caaatttctt
    24901 cgcctgttta aagttcgaaa ccggcaaaat tcccggaatg atttccacat caatgcctgc
    24961 cgagacgcag cgatcgcgaa aacgcaggta gctttcgaca tcgaagaaga actgagtaat
    25021 ggcgcggttg gctccggcat ccacttttcg tctcaggttg agtaaatcgg cctgggcact
    25081 tttcgcttct ggatgtactt ccggatacgc cgccacggag atatcgaaat ctgccacttc
    25141 ttttaacagt gtcaccaggt cagaagcata catttctggc ttaccacttc ccggcggcag
    25201 gtcgccacgc agcgccacga tatggcgaat accgttattc cagtagtcgc gtgcaatggt
    25261 ccgcagctcg gcaggcgtcg catcaataca ggtaagatgc ggtgccgctt ccaggccggt
    25321 acgatcttta atgcctttaa taatgctgtg cgtacggtcg cgctcgccgg agtttgcgcc
    25381 ataggtcacc gatacaaact tcggtttcag gctgctaagg cgatcgatgg agttccacag
    25441 ggtctgctcc atttcactgg tacgcggcgg gaaaaattcg aacgaaacgt taatctgccc
    25501 ctggacttct gccagactct gattcagggc atcccgctgg ctggcgtgaa aaaagctcat
    25561 accttacctc atcaatcgct tgtcgttata tgttgtgttg tgcacatcta tccgtttaga
    25621 cgtccagatg taaagatgaa ggaaaagccg aagggcgtca accgaaaaat aaccaatgat
    25681 ggtgaggaaa aatcagggaa gatgaaaaaa actcatctta attagaggcg ataaaagatt
    25741 tctctatagc gcaatccacc tcgcacgttt tgactcatat tctgacaaca cagcgacacc
    25801 tgggctaatt cgaaaaacaa cgtcctgcca ccggatggtg atgttaaggc atctgattcg
    25861 gcctgcgccc tcgcgtaggc cgaatacagg atctgcgcaa tcaacgggta acaaacagtt
    25921 gagtcaattc agtgcgcact tttatttgat atccttcacg cgcataccgt tgagctgttc
    25981 atccgtaatg gtgttgcccg ctttgaagta atcaaccacc gcgtgataaa cgtaataacc
    26041 gcccgttgtg ttacgcgctt tcccttcggt aaaggcggta aaaccatctc caccatcagc
    26101 aaggaaactc tgagtagcaa tgtgataaac cgtcgcatct tcaatgggtt tgccattcag
    26161 agtcagtgtg attacccgct gaccaaccgg cttgctgctg tcgtacttca tttccaggcc
    26221 tttcgatacc tgtaaaacgc cattgcttaa acttgcgccg tgttccatca gactgcgtaa
    26281 ttgtttaccc gtgagttcca tcgtcaccag ttcgttgggg aacgggaacg tactgatgac
    26341 gccgcccatc gtaatcgccc ctgcggggat ctcattgcgg ataccaccag agtttgttaa
    26401 cgccagttgg gtatttttac ccgctgctgc cagtaaagcg tcagccgcca ggttcccgag
    26461 ggaagccgat tcaccatagg cacgttttaa ttcaaccggc gattgcgcca ccgtttgttg
    26521 caccacttca tcaagctttt tattccagcc gtcgatcacc tgtttcgttt gctgatcggg
    26581 cttccactcg tcagcgtaaa tggttttaag ctcgaagttt ttcaccgtaa aattatgcgg
    26641 cttctctttg tagtcgagaa ccagtttgcc cacatcaatc ccgccgctgt cagtggagag
    26701 gatgagcgta ttgccgactt taatcggttc cggcgtaccc acatgtgcat gtccggtgat
    26761 caaaatatcc agccctttca cctgacttgc cgtctgaata tctttatcca acgcgcgacg
    26821 cacatctgtg cctcccatac tggactggcg ggccggaaca ccttcgtgga tcagggcgac
    26881 ggtcaaatca accttgcctt tgagttcatc gatataacgt tgtagccatt taatttcatc
    26941 gcgcgcttca atacccactc gcgtggcagc agataccgta tcattgaagg caaatactcc
    27001 gtgcaaacct ataacgccaa ttttcacgcc gtctttttcg atgatggtat
//
LOCUS       DAZGJT010000064        26473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZGJT010000064 DAZGJT010000000
VERSION     DAZGJT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.064"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(234..1634)
                     /gene="asnS"
                     /locus_tag="ACHKFK_002926"
     CDS             complement(234..1634)
                     /gene="asnS"
                     /locus_tag="ACHKFK_002926"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO9053217.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(1803..3005)
                     /gene="pncB"
                     /locus_tag="ACHKFK_002927"
     CDS             complement(1803..3005)
                     /gene="pncB"
                     /locus_tag="ACHKFK_002927"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO9053218.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQVQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLENKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQFGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            3271..5883
                     /gene="pepN"
                     /locus_tag="ACHKFK_002928"
     CDS             3271..5883
                     /gene="pepN"
                     /locus_tag="ACHKFK_002928"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO9053219.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDVPLRLNGEDLKLVSVYINDEPWTAWKEEEGALVISNLPERFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAVELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(6078..6845)
                     /gene="ssuB"
                     /locus_tag="ACHKFK_002929"
     CDS             complement(6078..6845)
                     /gene="ssuB"
                     /locus_tag="ACHKFK_002929"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9053220.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVA
                     LGLKGQWRDAARQALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWLKHGFTVLLVTHDVSEEVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMQRGHSEQPIRRHG"
     gene            complement(6842..7633)
                     /gene="ssuC"
                     /locus_tag="ACHKFK_002930"
     CDS             complement(6842..7633)
                     /gene="ssuC"
                     /locus_tag="ACHKFK_002930"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO9053221.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVMAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVLFALGLMWLTLIVAETISANSGIGYLAMNALEF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            complement(7645..8790)
                     /gene="ssuD"
                     /locus_tag="ACHKFK_002931"
                     /pseudo
     CDS             complement(7645..8790)
                     /gene="ssuD"
                     /locus_tag="ACHKFK_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(8787..9746)
                     /gene="ssuA"
                     /locus_tag="ACHKFK_002932"
                     /pseudo
     CDS             complement(8787..9746)
                     /gene="ssuA"
                     /locus_tag="ACHKFK_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
     gene            complement(9739..10314)
                     /gene="ssuE"
                     /locus_tag="ACHKFK_002933"
     CDS             complement(9739..10314)
                     /gene="ssuE"
                     /locus_tag="ACHKFK_002933"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO9053222.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSTLKAQEILHGVFADDSQVIDYQHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            10551..11561
                     /gene="pyrD"
                     /locus_tag="ACHKFK_002934"
     CDS             10551..11561
                     /gene="pyrD"
                     /locus_tag="ACHKFK_002934"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO9053223.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KTYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQVMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSQELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            11735..12277
                     /gene="zapC"
                     /locus_tag="ACHKFK_002935"
     CDS             11735..12277
                     /gene="zapC"
                     /locus_tag="ACHKFK_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO9053224.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWMPMPGDAAYVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(12274..13383)
                     /gene="ycbX"
                     /locus_tag="ACHKFK_002936"
     CDS             complement(12274..13383)
                     /gene="ycbX"
                     /locus_tag="ACHKFK_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO9053225.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHTLADVSGLAFDRIFMITEPDGT
                     FITARQFPLMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAGA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGICRVQ
                     LLEGEVTPLKKSAIGDDGTILCCSCVPKTALKLAR"
     gene            13481..15589
                     /gene="rlmKL"
                     /locus_tag="ACHKFK_002937"
     CDS             13481..15589
                     /gene="rlmKL"
                     /locus_tag="ACHKFK_002937"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO9053226.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWTQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVNDVAQLANPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMAA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRDFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            15601..17508
                     /gene="uup"
                     /locus_tag="ACHKFK_002938"
     CDS             15601..17508
                     /gene="uup"
                     /locus_tag="ACHKFK_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9053227.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELHNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            17638..18891
                     /gene="pqiA"
                     /locus_tag="ACHKFK_002939"
     CDS             17638..18891
                     /gene="pqiA"
                     /locus_tag="ACHKFK_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO9053228.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            18896..20536
                     /gene="pqiB"
                     /locus_tag="ACHKFK_002940"
     CDS             18896..20536
                     /gene="pqiB"
                     /locus_tag="ACHKFK_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO9053229.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     LLDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKGKKDPEPKRAKQ"
     gene            20533..21096
                     /gene="pqiC"
                     /locus_tag="ACHKFK_002941"
     CDS             20533..21096
                     /gene="pqiC"
                     /locus_tag="ACHKFK_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO9053230.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVAVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGMQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            21351..21518
                     /gene="rmf"
                     /locus_tag="ACHKFK_002942"
     CDS             21351..21518
                     /gene="rmf"
                     /locus_tag="ACHKFK_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO9053231.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(21588..22106)
                     /gene="fabA"
                     /locus_tag="ACHKFK_002943"
     CDS             complement(21588..22106)
                     /gene="fabA"
                     /locus_tag="ACHKFK_002943"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO9053232.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTAND
                     LKVGLFQDTSAF"
     gene            complement(22175..23935)
                     /gene="ycbZ"
                     /locus_tag="ACHKFK_002944"
     CDS             complement(22175..23935)
                     /gene="ycbZ"
                     /locus_tag="ACHKFK_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9053233.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGGHYEVSGHTIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQTIYSEFEDTLQIVDAESVSQWCRWVTFTAKHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCQEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEDKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            24121..24573
                     /gene="matP"
                     /locus_tag="ACHKFK_002945"
     CDS             24121..24573
                     /gene="matP"
                     /locus_tag="ACHKFK_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO9053234.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDEL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(24651..25691)
                     /gene="ompA"
                     /locus_tag="ACHKFK_002946"
     CDS             complement(24651..25691)
                     /gene="ompA"
                     /locus_tag="ACHKFK_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO9053235.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IDNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDVYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEVAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFTFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQALSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(26047..26373)
                     /gene="sulA"
                     /locus_tag="ACHKFK_002947"
                     /pseudo
     CDS             complement(26047..26373)
                     /gene="sulA"
                     /locus_tag="ACHKFK_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
     gene            complement(26391..>26473)
                     /locus_tag="ACHKFK_002948"
     CDS             complement(26391..>26473)
                     /locus_tag="ACHKFK_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9053236.1"
                     /translation="KSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         6631 a         6651 c         6917 g         6274 t
ORIGIN      
        1 agacatccag aatgccaaaa atttgtatga aaattgtgct atcagctaag aaaaaactaa
       61 tccgcaataa gaatgcagat tattttttgt gcgtaaatgt tgcagcgtaa tattccgtaa
      121 caggatgatc gttatggcgg ggttgcaaat agattgcttg acttaacaaa cagataactt
      181 gacagaaagg ataaaaaaag agccagcggg cgctggctct tgaagacgct aaattagaag
      241 ctggcgttac gcggagtacg tgggaacgga ataacatcac gtacgttttg cacgccagtc
      301 acgtaagcaa tcaggcgttc aaaaccaagg ccgaaacctg aatgcggaac agtaccgtag
      361 cgacgcagat cgcgatacca ccagtaatct tctttgttca ggcccatttc aagcatacgc
      421 tcgtccagca cgtccagacg ttcttcacgc tgggagccac caatgatctc accgatgccc
      481 ggagccagaa cgtccatagc cgcaacggtt ttaccgtctt cgttaaggcg catatagaac
      541 gctttaatat ctttcggata gtttttaacc actaccggtg ctttaaagtg ttcttccgcc
      601 agataacgct catgctcaga agagagatcg acgccccagt aaaccgggtt ttcaaacttc
      661 ctgccgcagt tttcgagaat ggtcactgcg tcggtgtaat ccacctgcgc gaaatcggcc
      721 tcaatgaagc gttccagacg tgaaacggca tctttatcta cgcgttcagc gaagaatttc
      781 atgtcgtcag cgcgttcttc gagaaccgct ttgaagacat atttcagcat ggcttcagcc
      841 agacccgcga tatcgttcag gttagcaaac gccacttctg gctccagcat ccagaattcc
      901 gccaggtgac ggctggtgtt ggagttttca gcacggaaag tcgggccgaa ggtataaatt
      961 ttggataatg cgcaagcgta ggtttcgccg ttcaactggc cagatacggt caggaaagac
     1021 tctttaccaa agaagtcttt gtcgaaatct actttgccct gatcgttacg cggcaggttt
     1081 tccagatcca gcgttgaaac acggaacatt tcgccagcac cttcggtatc agatgcggta
     1141 atcagtggcg ttgaaaccca gaagaatccc tgctcgttaa agaagcgatg cagcgcctgc
     1201 gccagcgtat ggcgaacgcg cgcgacggca ccaatcaggt ttgtgcgcgg acgcaggtga
     1261 gcgacttcac gcagatactc aatgctgtgg cgttttgccg ccatcgggta agtgtctgga
     1321 tcttcaaccc aaccagcaac ttcaaccttg ctggcctgaa tttcaaattg ttgcccctgg
     1381 cccggcgacg ccacgacttt acccgtcaca atgaccgagc agccggtggt cagacgcaag
     1441 acgtcttcat tgtaattggg cagagaatta ttgatgacag cctgtacagg atcaaagcag
     1501 gaaccgtcat aaacggcgag gaaggagatg ccagcttttg aatctcggcg ggtacgtacc
     1561 catccgcgca cggtgacttc gctgtcaacg gctacgcggc cctggagtac gtcggctaca
     1621 ggcacaacgc tcataatatt ctctctgtta atagtcggaa aaaataaaca cttgtccacc
     1681 cgtaaatggg gggataccta tgttacctgg catctgcaat cagacaagca gaattcgcaa
     1741 atgcagcgaa agatttcgga attaaaggta aaaaaaggga gccgattagc tccctgatga
     1801 tattaactgg cttttttaat atgcggaagg tcgaacgctt tgcgcagcgc ccgaacaaac
     1861 gctttatcgt ggcagatagt tttgccaggg ctgtcagaaa gtttcgccac cggtttaccg
     1921 ttacactcta ccaacttaat gacaatattc agaggtttta cctgggggat atcgcaggtc
     1981 aggcgagtcc caataccaaa acttaattgc acgcgggaag agaagtggcg gtatagctca
     2041 accgctttgc gtaaatccag attgtcagag aaaaccagcg ttttactctg tggatcaatt
     2101 cccagctttt cataatgtgc aatggctttt tcaccccatt caaccgggtc accagagtca
     2161 tggcgcaggc cctgatagcg actggcgaac tcgacaccga aatcacgcag gaaagcatcc
     2221 atagtgatgc agtcggttaa tgcaatgcca aattggtcgg gatactcttc cagccaggca
     2281 gcaagtgcag ctcgctggct gttggctaga tccgggctga tttgctgatg cgcctggaac
     2341 cattcgtgtg cctgtgttcc cattggcgtg agggaaagcc gacgcgccag atcgtagttg
     2401 ctggtgccca cgaaccagga ttcctgttgc agacgtttca cgatggtttc ttgtacttcg
     2461 cgagaaaaac ggcggcgggt gccaaaatcc atcagatgga agcgcgacat atcaagaccg
     2521 gcggttaatg ccgagaagtc gactaatttg ttttccagcg tgtcgagggc ttgcgcgacg
     2581 tcggcctgcg gtgagcgata gcgatgtacc atttcactga taaccgccag caaaggaacc
     2641 tcccagagga tgacttcacg ccacgggccg cttaaacgaa tatccagctt gccattatcg
     2701 ttggacacgg tgacttgttc cgggttaaag cggaactcgc gtagccagtt aagatagtcg
     2761 gccttaaaga aaggcagggc agagagccac tgatattcat catcctgcag gcgcaggtgc
     2821 tgcatcgcct gaacctgttc acgaatagca tcggcataaa tacccagcag atcgtcacct
     2881 cggcaacgaa actccgccgc gacatgcaca tcgtaatagt gatgaaacac ggcttgctgc
     2941 atatgcaact tataagcatc tgtatccagc aacgagtgca gaacaggaga agcgaattgt
     3001 gtcataggtg cgcagtagcg tcctcgtaca ggagcgttta gtacaataaa catcttcagg
     3061 aactgctgga gtataccttg tttcgcaatt tattgaaccc cgatcacacc atatgccacc
     3121 tttctggtcg atggcataac gcggtgtatg ttataaaaat gtagcaataa aggcgtttgt
     3181 acctgaaaag atgaagattc tgcatagcgc gatttacgca acaggaatag actgaacacc
     3241 agactctata aaagatgcta aaggttattt atgactcaac agccacaagc caaataccgt
     3301 cacgattatc gtgcgccgga ttaccagatt actgatattg acttgacctt tgacctggac
     3361 gcgcaaaaga cggtcgttac cgcggtcagc caggctgtcc gtcatggtgc atcagatgtt
     3421 ccgcttcgtc tcaacggcga agacctcaaa ctggtttctg tttatattaa tgatgagccg
     3481 tggaccgcct ggaaagaaga agagggcgca ctggtcatca gtaatttgcc ggagcgtttt
     3541 acgcttaaga tcgttaatga aattagcccg gcggcgaata ctgcgctgga agggctttat
     3601 cagtcaggcg atgcgctttg cacccagtgt gaagccgaag gtttccgcca tattacgtat
     3661 tatctcgacc gcccggatgt gctggcgcgt tttaccacca aaattattgc cgataaaacc
     3721 aaatatccct tcctgctttc caatggtaac cgcgttgcgc aaggtgaact ggaaaatgga
     3781 cgtcattggg tacagtggca ggacccgttc ccgaaaccgt gttacctgtt tgcgttggtg
     3841 gctggtgatt ttgatgtact gcgcgatacc ttcactaccc gctctggccg tgaagtagcg
     3901 ctggagctgt acgtcgatcg tggtaacctt gatcgcgcgc cgtgggcgat gacctcgctg
     3961 aaaaactcca tgaaatggga tgaagaacgc ttcggcctgg agtatgacct cgacatctat
     4021 atgatcgtcg cggtggattt cttcaatatg ggcgcaatgg agaataaggg gctgaatatc
     4081 tttaactcca aatatgtgct ggcccgcacc gacaccgcca ccgacaaaga ttacctcgat
     4141 attgaacgtg ttatcggcca tgaatatttc cataactgga ccggcaaccg tgtcacctgt
     4201 cgcgactggt tccagctcag tctgaaagaa ggtttaaccg tcttccgcga tcaggaattc
     4261 agctctgacc ttggttcccg cgcggtaaac cgtatcaata atgtgcgcac catgcgcgga
     4321 ttgcagtttg cagaagacgc cagcccgatg gcgcacccaa ttcgcccgga tatggtcatt
     4381 gaaatgaaca acttctacac cctgaccgtt tacgagaagg gcgcggaagt gattcgtatg
     4441 atccacactc tgcttggcga agaaaacttc cagaaaggga tgcagctcta tttcgagcgt
     4501 catgatggta gcgcagcgac ctgtgacgac tttgtgcagg cgatggaaga tgcgtcgaat
     4561 gtcgatcttt cccatttccg ccgttggtac agccagtccg gcacgccggt tgtgactgtc
     4621 aaagacgact acaatccgga aactgagcag tacaccctga ccatcagcca gcgcacgcca
     4681 gctacgccgg atcaggcaga aaaacaaccg ctgcatattc cgtttgccgt cgaactgtat
     4741 gacaacgaag gcaaagtgat cccgttgcag aaaggcggtc atccggtgaa ttccgtgctg
     4801 aacgtcaccc aggcggaaca aacctttgtt tttgataatg tctacttcca gccggtgcct
     4861 gcgctgctgt gcgaattctc tgcgccagtg aaactggaat ataagtggag cgatcagcaa
     4921 ctgaccttcc tgatgcgtca tgcgcgtaat gatttctctc gctgggacgc ggcgcaaagt
     4981 ttgctggcaa cctacatcaa gctgaacgtc gcccgtcatc agcaagggca gccgttgtct
     5041 ctgccggtgc atgtggctga cgctttccgc gcggtgctgc tcgatgagaa gattgatccg
     5101 gcgctggcgg cagaaatcct gacgctgcct tccgtcaatg aaatggcaga actgttcgat
     5161 atcatcgacc cgattgctat tgccgaagta cgcgaagcac tcactcgtac tctggcgacc
     5221 gaactggcag atgagctgct ggctatttac aacgcgaatt accagagcga gtaccgtgtt
     5281 gagcatgaag atattgcaaa acgcactctg cgtaatgcct gcctgcgctt cctcgctttt
     5341 ggtgaaacgc atctggctga cgtgctggtg agcaagcagt tccacgaagc gaacaatatg
     5401 accgatgcgc tggcggcgct ttctgcggcg gttgctgcac aactgccatg ccgtgacgcg
     5461 ctgatgcagg agtacgacga caagtggcat caggatggtc tggtgatgga taaatggttt
     5521 atcctgcaag ccaccagccc ggcggcgaat gtgctggaga cggtacgtgg tctgttgcag
     5581 catcgctcat ttaccatgag caacccgaac cgcattcgtt cattgattgg cgcatttgcg
     5641 ggcagcaacc cggcagcgtt ccatgccgaa gatggcagcg gttatcagtt cctggtggaa
     5701 atgcttaccg acctcaacag ccgtaacccg caggtggctt cacgcctgat tgagccgctg
     5761 attcgcctga aacgttacga tgccaaacgt caggagaaaa tgcgcgcggc gctggaacag
     5821 ttgaaagggc tggaaaatct ctctggcgat ctgtacgaga agatcaccaa agcactggct
     5881 tgatactttt gagccaaaat gcttgacgcg tcttatcagg cctacaagtc cgttgaaatt
     5941 gctggttttg taggctggat aaggcgttca cgccgcatca ggcagtcgtg cccaactgcc
     6001 tgatgcgacg cttgacgcgt cttatcaggc ctacaatccc gttgaaaatg ctggttttgt
     6061 gcgccacatc cggcatatta accatgacgt cgaatcggtt gttcagagtg cccacgttgc
     6121 atcacccgct gcaacacttc cgcttccagc tctgctaatc gtaccgaccc taaccggcgc
     6181 ggacggggga tatccaccgt caaatccaga ccaatttttc cctcttcaat taacaacacc
     6241 cggtcagcca tcgccacctc ttcgctcaca tcatgcgtca ccagcaaaac ggtaaaaccg
     6301 tgcttcagcc aaagcgacac aatcaaatcc tgcatctcga gtcgcgttaa agcgtccagc
     6361 gcaccgagtg gttcatcaag caacaataaa cctggtcgat gaatcagcgc ccttgccagc
     6421 gccacacgtt gtttctgccc gccagaaagt gcggcaggcc attcccccgc gcgattctcc
     6481 agtcctaccg cagccagagc ctgacgcgcc gcatcgcgcc actggccttt aaggcctaac
     6541 gcaacgttat caatcaccga tttccacggc agcagacgcg cgtcctgaaa catcattcgc
     6601 gtatcatcct gaatctcagc cagcggtgtg gtgcccgcta acacgtcgcc tgcggttggc
     6661 gtttccagac ctgccagcag gcgcagcaga gtacttttgc caccgccgct gcggcccacc
     6721 accgccacaa actgacctgc cggaatatgt aaatccagtt ggttcaggac gatattttcc
     6781 gcgtaatgtt tgcttactgc attgagcaac aatggtgtgc cctggttcag acgagccgta
     6841 ttcataccgt ggcctccttc aaatgataag ccgggttcca gcgcaaccag agacgttcta
     6901 acagctgcgc gctgacgtca gcaagtttgc cgagcagggc gtaaagaata atagcgacta
     6961 ccaccacgtc cgtttgcaga aactccagtg cattcatcgc cagataacca atgccggaat
     7021 tggcagaaat agtttcggca acgatcagcg tcagccacat caggcccaac gcaaaaagca
     7081 caccgaccat aattgagggc agggcaccag gcaggatcac atggataaac agcggtatgc
     7141 cggataatcc atagctacgt gccatctcca ccagcccacg gtcgatatta cggatgccat
     7201 gccaggtgtt gatataaatg gggaatagcg taccgagcgc caccagaaat atttttgcgg
     7261 attcatcaat gccgaaccac aaaatcacca gcgggatcag cgccagatgc ggcacgttgc
     7321 gcaacatctg aatggaggta tccagcagcc gttctcccca gcgcgacagc ccgctaatca
     7381 gccccagaat taatcccagc gatccgccaa ttgaaaaacc aatcagcgca cgccaggagc
     7441 tgatcgccag atgctgccac agttcgccgc tggcagagag cgtccagaac gccatcacca
     7501 ctccttccgg tgagggcaaa atacgcgtcg atagccagcc aaccgaggag gccagttgcc
     7561 acaccgccac gatgcccacc ggtaaaaacc agggagcaac gcgcaataac cacttcttca
     7621 ctggcgttgc catcatcgtg ttccttagct ttgcgcgacg ttacggggga taaaatcatt
     7681 cgccaccgct tcgccttgtg gattcagcgg ttgtggctgg ggaatttccg ggatggcgac
     7741 atccagatgt gggaacagta actcgccgac ccgatacgct tcttccagat gcgggtagcc
     7801 cgaaagcaca aaactgtcga tgccaagtgc agcgtattcg ttgattcgcg cgacgaccgt
     7861 aggaccatcg cccaccagcg ccgtcccggc accgccgcgc actaagccaa cacccgccca
     7921 taaattgggg ctgatctcca gattgtcgcg cttgccgtta tgtagcgccg ccattcgctg
     7981 ttgtcctacg gaatccgtgc gggcgaatgc ggcctgtgct ttggcgatag tttcatcatc
     8041 aagatgcgag attaaccgct cggcggcctg ccacgcttcg tcgttggttt cacgaacaat
     8101 cacatgcaga cgaataccga aacgaatttt gcgtccatgc gcggcagctt tcgcccgcac
     8161 gtgttcgatt ttctctttta ccagttccgg tggttcgccc caggtaaggt agagatcaac
     8221 ttgttctgcc gccagctcct aggcgacatc tgacgatccg ccaaagtaaa gcggaggata
     8281 aggctgttga atcggcggga agagcagttt ggctccgcgt acatgaatat gtttgccgtt
     8341 gaaatccaca gtctcgccaa gcaacaaccg ccgccagacc tgggtaaatt ctgccgaggc
     8401 ttcgtaacgc tcgctatgat caaggaacac gccgtcgcct gccagttctt gtggatcgct
     8461 gcctgtgacc aggttaaaca acgcacgtcc gttagagaga cggtcaagcg tggcggcctg
     8521 acgggcggca acggtaggtg aggttacgct ggggcgcagg gcgacaagaa acttcagccg
     8581 ttgtgtcacc gggatcatcg acgccgccac cagccacgca tcttcacacg atcgccccgt
     8641 cgggatcagc acaccggtat agcccagacg atccgccgct tgcgcaattt gttgcagata
     8701 accgtgatca accggacgtg aaccttcttc cgttcccaga taatgcccgt caccgtgggt
     8761 cggtaaaaac cagaacatat tcagactcat aattgttttc cttccagttg agtgggctgc
     8821 cagatgcgct ggcgaatatc gacttttttc ggcaccagac ggttttcata aaacagatct
     8881 gccgtttgct gctgtaaggc ggcaacttca gcgctcaacg gtttgatggt ggtgggagga
     8941 cgatgatcca gatatgaggc aataaccggt gccggtaagc ccatcgtttt tgccagtaaa
     9001 gcgatgcttt gttcgcgctg gctgcgggtt aacgcatcgg cctcactaaa ggttgtcagc
     9061 acaccctgaa taaaagcgcc gtttttttct gcatacggac gagctgccag ataaaacgat
     9121 ccagtttgat tgagatcggt gccgtctttc agcacccgca cgccgccctg taataatgca
     9181 gcggagtagt agggatccca gatagcctag gcgtcaacgt taccttgctg gaacgcggcg
     9241 cgggcatcgg caggcgtcag gtaagtgggc tgaatatccg tgaatttaag cccggcctgg
     9301 cgaagggctc gcagtaaaag attgtgcgaa ctggaacctt tctgaaaggc aactttgtga
     9361 cctttaagat cggctacggt tttgatcggg ctgttttctg ccaccagaat cacttcggct
     9421 ttgggcttcg gtggctcgac gcccacgtat accaaatcag ccccggcagc ctgggcaaag
     9481 attggcggaa tatccccggt actgccgaga tcgatactgc caacgtttaa cgcctccagc
     9541 atctgtgggc cagcggggaa ctccacccag gagatttttg tttgcggata gcgtttttcc
     9601 agtaactggt ggctttttgc cagcaccata ccaatactgc ctttttgata gcctatacgt
     9661 aacgcctctg gcgaagactc cgcggcaaca gcaagtgtag agacgctaag taaacccgcc
     9721 agcgccagtt taatgatctt acgcatgggc attacctcgc agagacagaa ggtcaggaac
     9781 ctgaacatcg cggcggtgca acgcctgcca gaaagtttct agcgcggtat caagacgagt
     9841 ttgcagattt ggcgtgaact ggggtttgtg ttggtaatca atgacttgcg agtcatcggc
     9901 aaacacgccg tgcaggatct cctgggcttt cagcgtactt aaaactggct taagggcata
     9961 atcgaccgcc agcagatggg ccacggtgcc gcctgtcgcc agcggcagca ccactttgcc
    10021 ttgcaacgca cgttctggca gcaggtcgag cagggttttc agtgcaccgg aataggcggc
    10081 tttatacaca ggcgtggcga caatcagccc atcggcctgt tgcagctgtt cggtgaaggt
    10141 cttgagtgcc ggactatcga aacgagcata aagcagatct tccgggacga agttttgcaa
    10201 attccagtga taaacctcta catccaggcc atttagtttt tcccgcgcat attccagcaa
    10261 ggagctggag cgagaaggaa agcgaggact acccgccagg gtgatgacac gcatactctc
    10321 tccttataac caattgttct tgttttgtta acattgataa caattcggtc agtctgacag
    10381 agggcgtccg cgagtcgaaa tgatttatct gaggaagaaa agccaaaaaa agcataaaaa
    10441 aagcgcaata agaaaacgtt tgctatttat tgccgcaggt caattccctt ttggtccgaa
    10501 ctcgcacata atacgccccc ggtttgcaca ccgggaatcc aggagagttc atgtactacc
    10561 ccttcgttcg taaagccctt ttccagctcg atccagagcg cgctcatgag tttacttttc
    10621 agcaattacg ccgtattacc ggaacgccgt ttgaagcact ggtgcggcag aaagtgcctg
    10681 cgaaacctgt taactgcatg ggcctgacgt ttaaaaatcc gcttggtctg gcagccggtc
    10741 ttgataaaga cggggagtgc attgacgcgt taggcgcgat gggatttgga tcgatcgaga
    10801 tcggtaccgt cacgccacgt ccccagccag gtaatgacaa gccgcgtctc tttcgtctgg
    10861 tagatgccga aggtttgatc aaccgtatgg gctttaataa tcttggcgtt gataacctcg
    10921 tagagaacgt aaaaaaagcc cattatgacg gcgttctggg tattaacatc ggcaaaaata
    10981 aagatacgcc ggtggagcag ggtaaagatg actatctgat ttgtatggaa aaaacctatg
    11041 cctatgcggg atatatcgcc atcaatattt catcgccaaa taccccagga ttacgcacac
    11101 tgcaatatgg tgaagcgctg gatgatctct taactgcgat taaaaataaa caaaatgatt
    11161 tgcaagtgat gcaccataaa tatgtgccga tcgcagtgaa gatcgcgccg gatctttctg
    11221 aagaagaatt gatccaggtt gccgatagtt tagttcgcca taatattgat ggcgttattg
    11281 caaccaatac cacactcgat cgttctctgg ttcagggaat gaaaaattgt gatcaaaccg
    11341 gtggcttaag tggtcgtccg cttcagttaa aaagcaccga aattattcgc cgcttgtcac
    11401 aggaattaaa cggacgcttg ccgattattg gtgttggcgg cattgactcg gttatcgctg
    11461 cgcgtgaaaa gattgctgcg ggtgcctcac tggtgcaaat ttattctggt tttattttta
    11521 aaggcccgcc gctgattaaa gaaatcgtta cccatatcta attatttctt caaattcctc
    11581 tttataagat aatcagggct ttattttcag ccctggttgt tttatattca tcgctgttgc
    11641 ttatttagac attttgtact tttattgttg aggttattaa gcgaagcgac aatggattgt
    11701 gtcgctgcgg ttttataagg ttggggagtg gcagatgcga attaaaccag acgataactg
    11761 gcgttggtat tacgatgaag agcacgatcg aatgatgctc gatttagcca atggtatgct
    11821 atttcgctca cgttttgcgc gcaaaatgtt gactccagat gccttttctc ccgcaggttt
    11881 ttgcgttgat gacgccgcgc tctatttctc ttttgaagaa aagtgccgcg attttaattt
    11941 atctaaagaa caaaaagccg aactggtgct gaatgcactg gtagcaattc gttatctcaa
    12001 accgcaaatg ccgaaaagct ggcattttgt atctcatggt gaaatgtgga tgcccatgcc
    12061 gggcgatgcg gcctacgttt ggttgagcga cactcatgag caagtgaatt tactggtcgt
    12121 tgaatctggc gagaatgccg cgctatgtct actggcacaa ccctgcgttg ttattgcggg
    12181 tcgcgcgatg cagttgggcg acgccattaa aatcatgaac gacaggctga aaccgcaggt
    12241 taatgttgac agcttcagcc tcgaacaggc agtctaacgc gccagcttaa gcgcagtctt
    12301 cggtacacaa ctacagcaaa gaatagtgcc atcatcgcct attgctgatt tttttagcgg
    12361 cgtgacttcg ccttctaaaa gctgaacacg gcaaattcca caaatgcccg cgcggcaaga
    12421 ataagggata cgaattccct gattttctaa ttgctccagc agcacctgtt ggttatttcc
    12481 acgaaatgcc tgtccctgcc agtcaatatc tacgtttgcg tccggttgtt gcgtgatgtt
    12541 ggcagtatca tcagcggcac ctgcgccgta aattttagcc ggagccgttg ccagaatttc
    12601 cacctcgtcg ccaacgcgaa tcacgccgct attacgggca attaaattct gaccaaaatc
    12661 gacatcgccg ttatcctggg cagtacggaa agattgcagc gtttttaatg gttcgcctgc
    12721 cggatgtttt tgcccttttt ctgggctgac ggtggtgaaa atacagcggc tacaaggctt
    12781 aaccacatca aacacgacat caccaatgcg aatcactttc cagctatctt cttcccaggc
    12841 cgaagcaccg gaaaccacca gattggggcg gaattgctcc atttttacac tggccggaca
    12901 acgttgttgg agatcacgta acgaggcctc gttagcaaga aggtaaggat agccatcagc
    12961 aaatgacagg ggtacagtgt tgtggcgttt cacgcgccgg gtcatttgtg gccccaccca
    13021 gcgtaattgc acttcgcggg agaaaaatcc acttagccat ttgttgatcg cgtctggcgc
    13081 aattcgcgcg gtaaaatgtg tgccccaaac ttcggttggt gcgtcttgtg tggcgaaatc
    13141 agcaaaacga acatatgcac tactgccatc tggtgcggtg agatgcaagc catcatgcac
    13201 gggtgaagga gtaaaccgta ccattagcgg aaactggcga gcggtaataa acgtaccgtc
    13261 aggttccgtg atcataaaga tgcgatcgaa ggccagacca ctgacatctg ccagggtatg
    13321 tgtaagacca atgccgcgca tcgatttaac aggatgaata aaaagccggg ttaatgtcgc
    13381 cacggtgcgg tcctcaaatg aaaataagcc ctcaacttta tgacatgacg cgcttattag
    13441 ctataatgcg caacaatttt cttagagtaa aagtgacaat atgaattctc tgtttgccag
    13501 tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg gggccgttga
    13561 atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg tttaccagag
    13621 cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt gtaaggttta
    13681 cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga tgtttaatcc
    13741 tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatacgca acagtcagta
    13801 cggtgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa atctgccgcg
    13861 tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc ataaagaaac
    13921 cgccagtatc gcccttgatc tcagtggtga tggtttacat ctgcgtggct atcgcgatcg
    13981 tgctggcatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc gatccggctg
    14041 gcagccagga acaccgctgc tcgatccaat gtgtggttca ggtacgttgc tgattgaagc
    14101 ggctatgttg gcgaccgacc gcgcaccggg cttgcaccgt gggcgttggg gctttagcgg
    14161 ctggacgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc aaactcgtgc
    14221 ccgtaaaggc ctggcggagt atagctccca tttctacggt tcggacagtg acgcacgggt
    14281 gattcaacgt gcgcgcacta acgcccgtct tgcggggatt ggtgaactga tcacctttga
    14341 ggtgaacgat gtcgcgcaac tggccaatcc gctgccgaaa gggccgtacg gtacagtgtt
    14401 gagcaacccg ccatacggtg aacgtctgga cagcgaaccg gcgctgattg cgctgcatag
    14461 tctgctgggc cgaatcatga aaaaccagtt cggtggctgg aatctctctt tgtttagtgc
    14521 ctcgccggat ctgctaagct gcctgcagct gcgtgcagac aaacagtaca aggcgaaaaa
    14581 cggcccgctg gactgcgtac agaaaaacta ccatgttgcc gaaagcacac cagacagcaa
    14641 accggcaatg gcagcggagg actacgccaa ccgtctgcgt aagaacctca aaaaattcga
    14701 gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg atctgccgga
    14761 atataacgtt gctgttgacc gttatgccga ctgggtggtg gtgcaggagt atgcgccgcc
    14821 aaaaactatt gatgcgcaca aagcgcgtca gcgtctgttc gatattatcg ctgcaaccat
    14881 ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac gccagaaggg
    14941 caaaaaccag taccagaaac tgggcgagaa gggtgagttt ctggaagtta ccgaatataa
    15001 cgctcacttg tgggtgaacc tgacggatta cctcgatacc ggtctgttcc tcgatcaccg
    15061 tatcgcccgt cgtatgcttg gtcagatgag caaaggcaaa gatttcctca acctgttctc
    15121 ttataccggc agcgccaccg tgcatgcggg attaggcggt gcacgcagca ccaccaccgt
    15181 ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga atggcttaac
    15241 tggccgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg aggcaaatga
    15301 acagttcgat ctgatcttta tcgatccgcc aaccttttct aactcaaaac gaatggaaga
    15361 tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac gtctgctgcg
    15421 tgcaggtggg acgatcatgt tctcgaacaa caaacgcgat ttccgtatgg atctcgacgg
    15481 tctggcgaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct cccaggattt
    15541 cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa aggaatagta
    15601 atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc gcttctcgat
    15661 aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtggggcg taacggcgca
    15721 ggcaaatcga cgttaatgaa gatcctcaac cgtgaacagg ggctggatga cggtcgcatt
    15781 atttatgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg taacgttgag
    15841 ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata cctgaaacgc
    15901 tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct caacgaactg
    15961 gcgaaggttc aggaacagct ggatcaccac aacctgtggc agctggaaaa ccgcatcaac
    16021 gaagtgctgg cgcaactagg attagatcct aatgttgcgc tgtcgtcgct ttccggcggc
    16081 tggttgcgta aagcggcatt aggacgcgcg ctggtgagca atccgcgcgt attgctgctc
    16141 gacgaaccga ctaaccacct ggatattgaa accatcgact ggctggaagg gttcctcaaa
    16201 accttcaatg ggacaattat tttcatttcc cacgaccgtt cgtttatccg caatatggcg
    16261 acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa ttacgaccag
    16321 tacctgctgg aaaaagaaga agccctgcgc gtggaagaat tacacaatgc cgagttcgat
    16381 cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg tcgtacccgt
    16441 aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgtg agcgtggtga acgtcgcgaa
    16501 gtaatgggta ccgcaaagat gcaggtggaa gaagccagcc gctccggtaa gatcgttttc
    16561 gaaatggagg acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga tttttctgcc
    16621 caggttctac gcggcgacaa aattgccctg attggtccga atgggtgcgg caaaaccacg
    16681 ctgctgaaac taatgctcgg tcagcttcaa gcagacagcg ggcgtattca cgttggcacc
    16741 aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga taaaacggtg
    16801 atggataacc ttgccgaagg taagcaagag gtgatggtta acggcaagcc acgccacgta
    16861 ttgggctatt tgcaggactt cctgttccat ccaaaacggg cgatgacgcc ggtacgtgcg
    16921 ctttctggcg gtgagcgtaa ccgcttgttg ttggcgcgtt tgttcctcaa accaagcaac
    16981 ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga actgctggaa
    17041 gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg tcagtttgtc
    17101 gataacaccg ttacagaatg ctggatcttc gaaggcggcg gtaaaattgg tcgttatgtc
    17161 ggcggttatc atgatgcccg tggtcagcaa gaacagtatg tggcgctcaa acagcctgcg
    17221 gtgaaaaaaa gcgaagaagc cgctgcgcca aaagcagaaa ctgtaaaacg cagcagtagc
    17281 aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct tgaagatctg
    17341 gaggcgaagc tggaagcttt acagacgcaa gtggcggatg cttccttctt cagtcagccc
    17401 catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga gctggagcaa
    17461 gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc gcaaaatagt
    17521 cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg tttatcaggc
    17581 atgaaaacca acactttgtt agtcatctgc aaaatagcgc atcattaagg agtaccaatg
    17641 tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat gctggtggcg
    17701 ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac aacgttaacc
    17761 gtggcgtggg atgcccctcg gcagcgtccc accgcctatg cgttggctgc actgttcatg
    17821 ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt taccagtgaa
    17881 attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag cctcggcacc
    17941 tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat tctgttactg
    18001 gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt gctttttcaa
    18061 ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag tttcgttaaa
    18121 ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg tttattttgt
    18181 gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga cgacatcgcc
    18241 ccgatgccag aactgcgcca gccgctaaaa ccaggtgtca cggggatacg tcaggggctg
    18301 cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg cccgcgttgt
    18361 agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc gctgcttgta
    18421 acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac ggatttatta
    18481 ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag cgaaggctct
    18541 tatcccgtcg ctgcggtgat ttttctggcc agtattatgg tgccaacgtt aaagatgatc
    18601 gccattgcgt ggctgtgttg ggatgccaaa ggacacggca agcgcgacag tgaaagaatg
    18661 catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga cgttttcgtt
    18721 atcgcggtgc tctcggcgct ggtacgtatg ggaggtttaa tgagtattta tccggcaatg
    18781 ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat gacgtttgac
    18841 ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg acagtatgga
    18901 atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg tgtggatatt
    18961 tcctatcgtc acggcgctca ttggggcctg ggttcttttt tatcattaca gccatcaggg
    19021 accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca aaaccaccat
    19081 taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg atgatttgac
    19141 acacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc ataaagatac
    19201 cgtcttttgg gtggtgaaac cgcagattgg tcgagaaggg attagcggcc tgggaacgct
    19261 gctgtctggg gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa tggataaata
    19321 cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc gtgtgcttct
    19381 cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc gtggctatcg
    19441 ggtaggttcg gttgaaacca gcactttcga tacgcaaaaa cgcaatatca gctatcaact
    19501 gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgtttct ggaaagatag
    19561 tggcattgcg gttgatctga cgtcagcggg aatgcgtgtg gagatgggct cattgacaac
    19621 gctgcttagt ggcggtgtca gctttgatgt gccggaaggt ctggatttag ggcagccagt
    19681 ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg attcgttgta
    19741 caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc tgcaaccggg
    19801 agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc cattctttgc
    19861 gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga ttcgtatcga
    19921 gccagagcgg ctgaaaatgc agcttggcga aaatgcggat gttgttgagc accttggcga
    19981 attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca ctggcgcact
    20041 gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc gtgaatttaa
    20101 tggttatcag attatcccga ccgtcagtgg cggcctggcg caaatccagc aacgactgat
    20161 ggaagcgttg gataagatca acaaactgcc actgaatccg atgattgaac aggcaaccag
    20221 tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg atagcatgaa
    20281 caagatcctt gccagccagt cgatgcagca gttaccgacg gatatgcagt caacgttgcg
    20341 tgaattgaat cgcagcatgc agggcttcca gcctggttcc gcagcctaca acaagatggt
    20401 ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc tgaaaacgct
    20461 caacgagaag agtaacgcgc tggtatttga agcgaagggc aaaaaagatc cagagccgaa
    20521 gagggcgaaa caatgaaaaa gtggctagtg acgattgcgg cactgtggct ggccggatgc
    20581 agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag cggtacacaa
    20641 agcaccgcca gccagggtaa tcgcctgtta tgggttgagc aggtcgctgt tcctgactat
    20701 ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat tgccaacaac
    20761 aacctgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc caacctgagt
    20821 acgcaactac ccggctgggt ggttgcctcc cagcctctgg gaagcgccca ggacacgctc
    20881 aatgttaccg tcacggagtt taacggtcgc tatgatggca aggtcattgt cagtggtgag
    20941 tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga aggaatgcaa
    21001 actcaggatg gttacgatga gatggttaaa gtgctggcca gtgtctggag tcaggaagcc
    21061 gcttctattg cgcaagagat aaagcgtcta ccttaatcat aaagatttgt aaatataacc
    21121 gcctccggta tgttgcctga ggcgtttttt tgtttctaac gtgcggaaaa agttgttcct
    21181 cttcacattt tttgtacaac cgacatgccc gtgtagctca caaatatgac agtggcgtga
    21241 attttgcgca ttgacggcag ttatgattcg cggtattgct taactgtgat tgcatattta
    21301 gtaatcactg ttttcttttc caccagaaac cagtatgagg gaaacgaggc atgaagagac
    21361 aaaaacgaga tcgcctggaa cgggcacatc aacgtggtta tcaggccggc atcgccggac
    21421 gctcaaaaga aatgtgtccc tatcagacgc tgaatcaaag gtcacaatgg ctgggaggct
    21481 ggcgagaagc catggcggac agggtagtaa tggcctgatt ctgtctcttt aaaaagaaac
    21541 ctccgcattg cggaggtttc gcctttatat actctgtctg attaaaatca gaatgcagac
    21601 gtatcctgga acagaccgac tttcaggtcg ttggcggtat agatcaaacg accatcaacc
    21661 agcacttcgc catcagccag gcccataatc agacgacggt taactatgcg tttaaagtga
    21721 atacgatagg tcactttttt cgccgtcggc agcacctggc ctgtgaattt cacttcacct
    21781 acgcccagcg cgcgaccttt accttcgccg cccagccagc cgaggtagaa ccctaccagc
    21841 tgccacattg cgtccaggcc caggcatccc ggcataaccg gatcgccgat aaagtggcat
    21901 ccgaagaacc acagatccgg attgatatcc agctctgctt caacataccc tttgtcgaag
    21961 ttaccacccg tttcggtcat tttgaccaca cggtccatca tcagcatgtt tggtgctggc
    22021 aattgcgggc ctttagcgcc aaacagttca ccgcgaccag aggcaagaag gtcttctttt
    22081 gtataggatt cgcgtttatc taccatgttc tctgtaagcc ttattttatt gaagcacgca
    22141 ggatagctaa cacgtgtacg ctgaacaagt ccgatcagtt cggaataaac cagttcagcc
    22201 aacgtaatgg ccatggaaaa cggtgacgtc cttcctgttg cgatgcttgt gcgatacgtt
    22261 cctggatggt ttgcatcagc gtcgtttggc cttcgccatc ccacaccaga tttaataata
    22321 acggcagtgc gtcagtcaca tcgtctactg cccagatggt gaatttgtct tcttctaccg
    22381 ctttcaccag ttcactgtga agacttaaat ggcgaacgtt agctgtgggg ataatgacac
    22441 cttgtttccc cgttaactca cgttgctggc aaatagcaaa gaagccttcg attttctcat
    22501 ttaaaccacc gactggctgg gcgcgaccga actgatcgac tgaacctgtg atagcgatac
    22561 tctgattcac cggcacatcg gcgagggcgc ttatcagggc gcagagttca gccatcgagg
    22621 cactatcgcc atccacttca ctgtatgact gctcaaatgt cagcgatgct gagaagggga
    22681 tctgttgctc aagctgtagt tccgacatca ggaacgcttg catgatcatc atccctttcg
    22741 catggatatt gccgccaagc tccgctttgc gttcgatgtc ggtgaattca ccatcgccaa
    22801 tatgcacaac gcagctaatg cgagaaggtt cgccaaaagc gcgtggatga cccggaaatt
    22861 caatgaccga aagggcgtta atttgcccga tgcgttcgcc ttcggtttca atcaggattt
    22921 gctcctgaag gatctcatcc tgcatgcgtt cagcgaggaa accttcgcgc cattcacgct
    22981 gctgtagcat taagtttagc tgctcgccgg agaaggtgtc gccatcacac agggaggcga
    23041 cctcttgaca ctggcggagg atccactgcg ggctaagcgg aagtgtttct tgttcaccag
    23101 tgtagcgtgc tgcttcgcgg ataagtaccg gccaggcatc cgctcccggt gctggtaagt
    23161 gattatgttt ggcggtaaat gtcacccagc gacaccactg gcttactgac tccgcatcga
    23221 caatctgcag agtatcttca aattcgctat aaatagtctg ctctgaaagc tctggctcca
    23281 tctcctggaa atcagccaat gattcgcgtt cgcctaccag aatgactttc agcttcaatg
    23341 gcatcgaagg cacagagacg gggagagggc gcgactcatc aaacgcaacc cagtcaaaac
    23401 gctcgcggtt aacgatattt ttcagccgca tccacagcag aggttgcgcc agcagtgtac
    23461 gcaaagagat gatgagaata ccgccatttg cctgatgcac cagaccaggc tgcagggtaa
    23521 tgtcgccatt aaactgacgc aggcagccaa agagttgctc tgcttctacc cagtcggcag
    23581 cgacaacttg cgttaaagtc gcaaaattat catctgcact cactgcgtga cgtaagcgga
    23641 tggtgtggcc ggaaacctca tagtgaccgc ccaccagttg gcctgcatcg ctttgtagcg
    23701 tacgcgcggc atcagcaata agattcagat actcagactc ttccggggcc ttcgccagca
    23761 taaaagagga ggatgctcgc gtatgcagca actgctccag cgcaaattgc agtcgcggtt
    23821 gagtatcact gaataaagga tcgttttcgt caatcaaatg tggctgagca aatatttcct
    23881 gatagctatc ggtatcagga accaggtcac gccatgcaag tttcgtaatg gtcaaagttg
    23941 atgtttttta gtctgttgtc aaagccgcga ttataccgta accggcactg cagcacacgt
    24001 agaaagcaac gacaatactc ttggcatggg cgttaaagct cacaggatgg agattctttt
    24061 cttcactggc ctaaaaagct gatattctgt aaagagttac acggtaacat tgagatcgct
    24121 atgaaatatc aacaacttga aaatcttgaa agcggttgga aatggaagta tctggtgaag
    24181 aagcaccgcg aaggggagtt aatcacccgt tacatagaag ccagtgccgc ccaggaagcc
    24241 gtcgatgagt tgctctcgct ggaaaatgaa cccgtgctgg taaatggttg gattgacaag
    24301 catatgaatc cggaactggt caatcgcatg aagcagacca ttcgggccag acggaagcgc
    24361 cattttaatg cagaacacca gcatacgcgc aaaaaatcga tcgatctgga atttatcgtc
    24421 tggcaacgtc tggctggcct tgcgcagcgt cgcggtaaaa cgctttctga aacgattgtt
    24481 cagctgattg aagatgcgga aaacaaagag aaatacgcga ataaaatgtc ttctttgaag
    24541 caggatctgc aggcattgct gggtaaggaa taactgtcaa aagtcagttc aatttactaa
    24601 aggcgaaaaa aaaaccccgc agctgcgggg tttttctacc agacgagaac ttaagcctgc
    24661 ggctgagtta caacgtcttt gatgccttta acttcgatct ctacgcgacg atccggagcc
    24721 aggcaatcga tcagtgcagc acgctgtttc acgttgtcac aggtgttgcc agtaaccggg
    24781 ttggattcgc ccataccacg tgcggagatt ttgtctgccg ggataccttt agagatcagg
    24841 taatcaacaa cggactgagc acgacgctcg gacagagcct ggttataagc gtcagaaccg
    24901 atgcggtcag tgtaacccag aacaactacg gaaccgtctt tcggatccag gttgctcagc
    24961 tggctgtaca gctgatccag agcagcctga ccttccggtt tcagggttgc tttgttgaag
    25021 gtgaacagaa cgtcagactt cagagtgaag tgcttggtct gtacttccgg tgccggagct
    25081 ggagccggag caactactgg agctacttcg ccctgaccga aacggtagga aacacccagg
    25141 ctcagcatgc cgttgtccgg acgagtgccg atggtgtgtg cgtcaccgat gttgttggtc
    25201 cactggtatt ccagacgggt agcgatttca ggagtgatcg catactcaac accgccagcg
    25261 aagaccggag aaacgccggt gtcgtggttt ttaccataaa cgttggattt agtgtctgca
    25321 cgccatacca taccacccag acgagtgtat acgtccagat cgtcagtgat tgggtaaccc
    25381 agtttagcgg tcagctgaac gccctgagct ttatatgcac cgttttcaac gctgcctttg
    25441 tatggcatac gacctaacca gtcataaccc atttcaaagc caacatacgg gttaacctgg
    25501 taaccaccaa aagcaccagc gcccagttgg ttttcatggg tcggaccatt gttgtcgatg
    25561 aaaccagtgt catggtactg ggaccagccc agtttagcac cagtgtacca ggtgttatct
    25621 ttcggagcgg cctgcgctac ggtagcgaaa ccagccagtg ccactgcaat cgcgatagct
    25681 gtctttttca ttttttgcgc ctcgttatca tccaaaatac gccatgaata tctccaacga
    25741 gataacacgg ttaaatcctt caccggggat ctgctcaata ttaactctac cgatatcttc
    25801 ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa acttacaagt
    25861 gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtacgttta atcttttttg
    25921 tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct ccgcaaggaa
    25981 aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga ttaatcctaa
    26041 atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc tcgttgcgtg
    26101 agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat ttgccgcatc
    26161 aacaagttca gcatgctctt ctgcagtcaa atcatctgcc aaccaaccga tcaccacgct
    26221 gtaattgccc gtgcgtaaag cgcgaaccat tgactccacg gtgtggcaag gggagagctg
    26281 gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt cgcgactcag
    26341 tttttgttgc ggtgttaacc agagttgcca gcgcgggtaa tgactccaac ttattgatag
    26401 tgttttatgt tcagataatg cccgatgact ttgtcatgca gctccaccga ttttgagaac
    26461 gacagcgact tcc
//
LOCUS       DAZGJT010000065        26379 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZGJT010000065 DAZGJT010000000
VERSION     DAZGJT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26379
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.065"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..200)
                     /locus_tag="ACHKFK_002949"
     CDS             complement(<1..200)
                     /locus_tag="ACHKFK_002949"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO9053237.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIV"
     gene            complement(232..501)
                     /locus_tag="ACHKFK_002950"
     CDS             complement(232..501)
                     /locus_tag="ACHKFK_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO9053238.1"
                     /translation="MLTKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTALFRDRFGEAE"
     gene            complement(491..1999)
                     /locus_tag="ACHKFK_002951"
     CDS             complement(491..1999)
                     /locus_tag="ACHKFK_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO9053239.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCSAFAPLVQPGEHAGWV
                     SESLCKTLGFSQSVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAVSLNRGYADYLA
                     GNWSLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLNIDMLISSVSLGLTMEFANC
                     FACFNVPGTSVLKVIGPATHNPLWLQLKADILQRPVEAIAFSEAVSVGALLTAAPDIP
                     PPQVTIAQRLLPNRARYHQLQRYQHKWESWYQLKLQQEGVMPLHHREEHYVD"
     gene            complement(1992..3350)
                     /locus_tag="ACHKFK_002952"
     CDS             complement(1992..3350)
                     /locus_tag="ACHKFK_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO9053240.1"
                     /translation="MNDIAHTLYNIVQYILGFGPTVMLPLVLFILALCFKVKPAKALR
                     SSLTVGIGFVGIYAIFDILTSNVGPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIA
                     PFVIPLTILINVAMLALNKTRTVDVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAA
                     VVVLKLADWSAPLVQKYFGLEGISLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPE
                     TVQKKFGIFGEPMMVGTILGILLGVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEG
                     LLPLSEAIKKYLNAKYPDRDDLYIGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGN
                     EVLPLGDLANLAVMASMIALASRGNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDV
                     NFKFAEGSSGQVSSFLDGGNPFRFWLLEIFNGNIIAIGLVPVIALVLYGIFRMTRSTV
                     YA"
     gene            complement(3424..3705)
                     /locus_tag="ACHKFK_002953"
     CDS             complement(3424..3705)
                     /locus_tag="ACHKFK_002953"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9053241.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKK"
     gene            complement(3702..4175)
                     /locus_tag="ACHKFK_002954"
     CDS             complement(3702..4175)
                     /locus_tag="ACHKFK_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO9053242.1"
                     /translation="MQGIQFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLTMETTEIHTVILNKMNERGEI"
     gene            complement(4200..4946)
                     /locus_tag="ACHKFK_002955"
     CDS             complement(4200..4946)
                     /locus_tag="ACHKFK_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9053243.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIEPANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIV
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRELPVEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(5145..5546)
                     /gene="nikR"
                     /locus_tag="ACHKFK_002956"
     CDS             complement(5145..5546)
                     /gene="nikR"
                     /locus_tag="ACHKFK_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO9053244.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(5552..6358)
                     /gene="nikE"
                     /locus_tag="ACHKFK_002957"
     CDS             complement(5552..6358)
                     /gene="nikE"
                     /locus_tag="ACHKFK_002957"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO9053245.1"
                     /translation="MTLLNVSDLSHHYAHGGFSSKHQHQAVLNNVSLALKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKAEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLMLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(6355..7119)
                     /gene="nikD"
                     /locus_tag="ACHKFK_002958"
     CDS             complement(6355..7119)
                     /gene="nikD"
                     /locus_tag="ACHKFK_002958"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO9053246.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(7119..7952)
                     /gene="nikC"
                     /locus_tag="ACHKFK_002959"
     CDS             complement(7119..7952)
                     /gene="nikC"
                     /locus_tag="ACHKFK_002959"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO9053247.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLIIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTTEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(7949..8893)
                     /gene="nikB"
                     /locus_tag="ACHKFK_002960"
     CDS             complement(7949..8893)
                     /gene="nikB"
                     /locus_tag="ACHKFK_002960"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO9053248.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLSD
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(8893..10467)
                     /gene="nikA"
                     /locus_tag="ACHKFK_002961"
     CDS             complement(8893..10467)
                     /gene="nikA"
                     /locus_tag="ACHKFK_002961"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9053249.1"
                     /translation="MFSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALGKNELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWVLQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(10578..11165)
                     /gene="acpT"
                     /locus_tag="ACHKFK_002962"
     CDS             complement(10578..11165)
                     /gene="acpT"
                     /locus_tag="ACHKFK_002962"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO9053250.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYNSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(11220..12269)
                     /locus_tag="ACHKFK_002963"
     CDS             complement(11220..12269)
                     /locus_tag="ACHKFK_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO9053251.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPRFNKELT
                     RKLFKLQEMLPFLNLHMSPERILQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGIIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            12368..13618
                     /locus_tag="ACHKFK_002964"
     CDS             12368..13618
                     /locus_tag="ACHKFK_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312349.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053252.1"
                     /translation="MKHCCKNVVILMPEPVAEPALNGLRLNLRIVSIVMFNFASYLTI
                     GLPLAVLPGYVHDVMGFSAFWAGVVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLC
                     GCFLSGLGYLTAGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIG
                     RVISWNGIVTYGAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASK
                     GKPLPFRAVLGRVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFV
                     GTRLLFPNGINRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFP
                     ALGVVAVKAVPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVA
                     IALLLTWRLKKRPPVEIPEAASSS"
     gene            complement(13622..14179)
                     /gene="dcrB"
                     /locus_tag="ACHKFK_002965"
     CDS             complement(13622..14179)
                     /gene="dcrB"
                     /locus_tag="ACHKFK_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO9053253.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADNQQKAQTTAENIINTLVIQ"
     gene            complement(14252..14917)
                     /locus_tag="ACHKFK_002966"
     CDS             complement(14252..14917)
                     /locus_tag="ACHKFK_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO9053254.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRHWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            15138..15383
                     /gene="tusA"
                     /locus_tag="ACHKFK_002967"
     CDS             15138..15383
                     /gene="tusA"
                     /locus_tag="ACHKFK_002967"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO9053255.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(15652..17850)
                     /gene="zntA"
                     /locus_tag="ACHKFK_002968"
     CDS             complement(15652..17850)
                     /gene="zntA"
                     /locus_tag="ACHKFK_002968"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO9053256.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNTNDCCCGGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQASDEPQESRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGTTSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAQSQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVCNDDVLGVIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRK"
     gene            complement(17924..18550)
                     /locus_tag="ACHKFK_002969"
     CDS             complement(17924..18550)
                     /locus_tag="ACHKFK_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO9053257.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            18691..19050
                     /locus_tag="ACHKFK_002970"
     CDS             18691..19050
                     /locus_tag="ACHKFK_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO9053258.1"
                     /translation="MSKPPLFFIVIIGLIIVSASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(19053..19322)
                     /locus_tag="ACHKFK_002971"
     CDS             complement(19053..19322)
                     /locus_tag="ACHKFK_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO9053259.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(19312..19908)
                     /gene="rsmD"
                     /locus_tag="ACHKFK_002972"
     CDS             complement(19312..19908)
                     /gene="rsmD"
                     /locus_tag="ACHKFK_002972"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO9053260.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            20058..21554
                     /gene="ftsY"
                     /locus_tag="ACHKFK_002973"
     CDS             20058..21554
                     /gene="ftsY"
                     /locus_tag="ACHKFK_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO9053261.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQIPEKETEVQNEQPVVEEIVQAQEP
                     VKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
                     AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            21557..22225
                     /gene="ftsE"
                     /locus_tag="ACHKFK_002974"
     CDS             21557..22225
                     /gene="ftsE"
                     /locus_tag="ACHKFK_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO9053262.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            22218..23276
                     /gene="ftsX"
                     /locus_tag="ACHKFK_002975"
     CDS             22218..23276
                     /gene="ftsX"
                     /locus_tag="ACHKFK_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO9053263.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEYRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            23521..24375
                     /gene="rpoH"
                     /locus_tag="ACHKFK_002976"
     CDS             23521..24375
                     /gene="rpoH"
                     /locus_tag="ACHKFK_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO9053264.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            24646..25749
                     /gene="livJ"
                     /locus_tag="ACHKFK_002977"
     CDS             24646..25749
                     /gene="livJ"
                     /locus_tag="ACHKFK_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO9053265.1"
                     /translation="MNMKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(25937..26320)
                     /gene="panM"
                     /locus_tag="ACHKFK_002978"
     CDS             complement(25937..26320)
                     /gene="panM"
                     /locus_tag="ACHKFK_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO9053266.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNYRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPDVSCWWMADAGV
                     EDRGVMTAFMRALGFTAQQGGWEKH"
BASE COUNT         6395 a         6898 c         7107 g         5979 t
ORIGIN      
        1 ttcacgattt catatatgta atcgtatccc gacagttgtg cattggtggg cgcaatctga
       61 atgaagttgg gtaatcccga acgctcaata gcgtttaaaa ttgaaattgt cgtctccaga
      121 ttggtggtat taaacgcgcc cgctaatacc cgtttttcct gaatgcggtc gagcagtatc
      181 cgtccattca ctaatggcat attgaaaacc tcatctttta atatcagtta attattcagc
      241 ttcaccgaag cggtcgcgaa ataaggcggt gagagtctgt atggcctcgt tttcatcatc
      301 accgtcgcag cgtatttcca ccgtttttcc gccttttatt ccggcgcgca taatattcat
      361 catactggtg gcattaatat cattaccctc ataatgcagc gtcactttac agctatactt
      421 tttggcggct ttcgccagac aggccgccgg acgtgcatgc aggccagtac tgtttctgac
      481 ctccacggtt ttagtcaaca tagtgctcct cccgatgatg taatggcatc acgccttctt
      541 gctgtaattt caactgatac cagctttccc atttgtgctg ataacgctgt aattgatgat
      601 atcttgcccg attcggtaac aaacgttggg ctatggtaac ttgcggcggc ggaatatccg
      661 gtgccgcggt taataaggct ccgacagaaa ccgcctcgct aaatgcaatt gcttcaaccg
      721 gacgctgtaa aatatctgcc tttaattgca gccaaagggg gttatgagtc gccggaccga
      781 tcacttttaa cacgctggtg ccaggcacat taaaacaggc aaagcagttg gcaaattcca
      841 tggttaatcc cagtgagacg ctggaaatta acatgtcgat atttaacgta tcgccaagcc
      901 cataaaataa tccgcgggtg tggcgatttt tatagggtga acccgaaccg cgaagatgcg
      961 gaataaagac aggaatgtca tcaagcgacc agttccccgc caggtaatcc gcatacccac
     1021 gattgagcga cacggcgatc tcctcatcgg ttagccggaa cgtgttacgc agccactcca
     1081 gcgcgaaacc gcccacgggc agagaagcaa acagggtgaa ctggttggca agcgagtaac
     1141 agccgtttgc cagcttgtca cgtttggcct gttcatccag cgtcggttgt gtatccagtt
     1201 gcagaatgcc ttccgtggtg cccgtcgagt taaggatatc gcccggcatc atctgcaacg
     1261 cccgcgcacc caccatatgg tcatgtccgg ccagcgtcac gctgaccgat tgcgagaacc
     1321 caagcgtttt acagagcgac tcactgaccc atccggcgtg ttcgcctggc tgcaccaatg
     1381 gcgcaaatgc tgaacaggga acgtgtaaca acgccgccgc tttcgctgac cattcgcctt
     1441 tgcgcacgtc caggcacagt gtacggctgg ctaaggtgat atcagttcgc tgttcgccag
     1501 tcagccgcca gagcagcact tccggtgcat gtagccagcg caggccgcga cgattgcgca
     1561 gcggataatg ctccagcaac caacgcattt tgaaggcaga gtaattgctg tgcagtggta
     1621 acccgcaaat gtcataaagt gccgcactgt ctgcctcgct aagcgttgcc agatactctt
     1681 caccgcgacg gtcataccat gccagcattg gcgtcaggat ctcgccatgt tcgtcaagaa
     1741 acacccctga ctcgccaaaa ctggcaatgc ttatccgcct gaccggcagc ggcgaagcgg
     1801 cgttcagttg cgctatcgcc tggcggacct cctgccagag ggcgtcgata tcgaaatcga
     1861 catcgccctg tggggagatc tgctttgccg tcacgaattt atgcgcgccc aacgtcgttg
     1921 cgtccaggca ggaaaagcag gtgactttgc aattggtggt gccaatatcg ataacgatag
     1981 ctgcgctgtt atcaggcata aaccgtgctc cgcgtcattc ggaaaatgcc atacagtacc
     2041 agggcgataa ccggcaccag accaatggcg atgatattgc cgttgaagat ttccagcagc
     2101 cagaagcgga acgggttacc gccatcaagg aaactggaaa cctggccgct ggagccttcg
     2161 gcaaatttga agttaacgtc tttcgccatg ccggtaataa acggtgcgat tttagtcgca
     2221 atccacaggt cggcaataat caccgggatc gccgccagaa cggtgcggaa aatattgcca
     2281 cggctggcta aagcaatcat cgacgccatt accgccaggt tggcaaggtc accaagcggc
     2341 aggacttcat tacctgggag gacaaacgcg ataaagaccg agattggcgt cagcagcagg
     2401 gcggtggaga taatcgccgg gttacctacg gcaacggcga tatccaggcc gatatagaga
     2461 tcgtcacggt cagggtattt ggcattgaga tactttttaa tggcttcaga cagcggtaat
     2521 aaaccttcca tcaggatgcg taccatgcgt ggcaggatga acatcacacc gccaatgctg
     2581 atgccaagca gcaagacttt tttgaaatcg tatccggcaa ttacgccgag cagaatgccc
     2641 agaatagtgc caaccatcat cggttcgccg aagatgccaa actttttctg tacggtttcc
     2701 gggtcgatat gaatacgatt gaggccaggg atatggtcga tgattttgtc gaccaacaga
     2761 ccgaccggaa agaacaccac cgaagagagc gtcggcaaag agatcccttc caggccaaag
     2821 tatttttgta ccagtggcgc agaccagtcg gcgagtttaa gtactaccac cgcagcaatc
     2881 gccgctgcca gcaaaccaaa gaacaggctg ccggtgctgt aataaaccag cgtaccggca
     2941 agggcaaaat gccagtagtt ccacatatct acgtcaacgg tgcgggtttt atttaacgcc
     3001 agcatcgcca cgttaatcag aatagtcagg ggaataacaa acggggcaat tggcgaaccc
     3061 catgtaatag cggaaagcgg cggccagcct aaatccacca ccggtaaatt aattccggtg
     3121 cgttcaacca tcgcctgggc cgctggcccg acattgctgg taagaatatc gaaaatggca
     3181 taaataccga caaaaccaat gccgaccgtt aatgacgagc gtaaggcttt agcgggtttt
     3241 actttaaaac agagagcaag aataaataac accaacggca acattaccgt ggggccaaat
     3301 cccaatatat attgcacaat attatagagg gtatgcgcga tatcgttcat aataaacctc
     3361 tttttcaggc gagagcgcac ctcgcgttag cgcctgatgg agtgggttgg aattaattat
     3421 taattacttt ttaagaatcg aaacaatttg cgccagtact ttctcttcgc caataccggt
     3481 aataatcggc aagccgttaa ctaccgggac gtcgagttca taaggcactt tagtcgttga
     3541 gactaccagt gctacgccat cagtatttcc aggtaaagag gcaacgttgg tttgggaata
     3601 attgacgtta agaccgtgct ctttgcaata ctccatgact ttttctgtta ccgccgtgga
     3661 tgtggcaata cctgtggcgc agacaaataa aatggtttga ctcatatttc acctcgttca
     3721 ttcattttgt ttaagattac tgtgtgtatt tctgttgttt ccatcgtaag caaggcttgc
     3781 ataaagggcg tatcctgaat catctccata atccagccca gggcatttaa ttgtttatta
     3841 ctttcactta aggcgagcaa aaaaatcacc ctgacaggta cggggtcagg atcgccgccc
     3901 atatcggcaa actctaccgg ttccggcagg atgccgacgg caattgcatt gtgccggaca
     3961 tgtttatggt ccgtgtgcgg aatggcgacg cacggtggtt cggtaggcag ccccgttggg
     4021 tacgttgcct cacgttttag cacgtgatcg gcaaaatcag ggaccaccag ctcagcagca
     4081 accaacggtt gcgctaactg acggatgatc tgttcaacgc ttaaccctgc cggaagtcgt
     4141 tgaatataat tttcctgaaa ctgtatgcct tgcattttat tcctcataaa aaagtgtatt
     4201 taactcactt cccgccgttg cagcacggtg cccagataat atttatttcc ttttaaccag
     4261 acgttgccaa actcaacggg taactctcgg gagaaaaaga ctaactgctc cagatgcaaa
     4321 acgggtgcat cttcactgat atctaaaaaa tgaccgcgtt cattaccaat taatcgcgcg
     4381 gcatagcgac tttccgagta acgaattttt ctttgcgaca aactttctat tgttggaaat
     4441 aaattgtgtt gattaaaatc gatttccacg atgccggggc atagctcaat attgatacgg
     4501 ttctctatca gcatcgcttt ttcatcacca attgaacgta aacgctcaag gtaaagaata
     4561 tcctgaccgg gcgttattct taatttctct gccacataac gattagccgg ttcaatccgt
     4621 gacgtaataa cttcagtggt aaaatgtatt ttctggcttt ccagcgattc cgcgaatgac
     4681 aataatcctt cacctaacgg atatgccacg ttctcttttt tgacaaaagt tcccttcccc
     4741 tggacctgaa tcaacacgcc ttcttttacc agttgggcga ccgctttttt aacggttccc
     4801 cggctcactt ccagcatatc cataatctgg tattccgaag gaatgcgatc gtcttccacc
     4861 agatccccgg tataaatact ttctcttatc cactccacca cttgttgata gagcggtatt
     4921 tgtgacgact tactcaatga tttcatggcg cgcacctgaa catttacagt tgtctatgga
     4981 caatcatgga caacttcaag cgtatagtag gtgaacaggc gaaaaagagg atcgaagcgt
     5041 ctcacatttt aggggaccaa atcgtgatca aaaaaatgtg acgtgagtcg atatttggca
     5101 gaagtctact gcgccgtcag gaaataacgg cgcgttggga ggtgttaatc ttccttcggc
     5161 aggcactgca aatgcccgtg ccgcacgccg cgctgggcga tgacgtcatc ggcaaaatgc
     5221 tgcacgtcgc ccatgtcacc tttcaacacg gcgatttcca gacagtcgtc gtgattaatg
     5281 tgcacatgca gagtagcaac cgagagatcg tggtgatgat gctgggtgga gacaatacgg
     5341 ctggctaagt cgcgtttttc gtgttcatac acatacgaca gcaccgcgaa accttgtgtg
     5401 ccgtgttgtt gagtggcttc ctgcgccagg gcgctacgca gaatatcgcg gatcgcctcg
     5461 gaacggttgt tataaccacg acgctggctc aggctgtcca gcgtctccag taaatcgtca
     5521 tcaagcgtga tggtgactcg ttgcatttgc gttaaacctt ttctgtggtg cgacggcgca
     5581 cggggaatgc gggtaatacc gcgttttgta gcacacgtcc ggcgtcagag gaaaaggtta
     5641 atttatctcc caccacctgg gtttcgacga tttgtccgtt gtccatcacc atcacccgct
     5701 gacagaagcg ttccaccagg cgtaagtcgt gggtgatgaa caggcaggcg gtgccaaact
     5761 gttgttgcag ctttttcagc aggcgaatga cgcccgcttg taacatgaga tcaaggttcg
     5821 aaaccgcttc atccagaatc agcagtttcg gttcgaccgc cagcgcgcgt gccaggcaga
     5881 cacgctggag ctggccgccg cttaactgcg gtgggcgttt gtcgagcacg ctgtcgtcga
     5941 gatcgaccgc cttcagcatt tcactggcgc gcgccagttg ttcggctttt ttcagcgata
     6001 gcaggtggcg catcggttca cgcaggatct cgcgcacggt tttgcgcgga ttcacggcgc
     6061 tgatggagtc ctgaaacacc atttgaatat cacggcggaa cgctttacgt tgcgcgcgat
     6121 tgagcttcgc caacggttcg ccgcgccagc taatatttcc ctgtgagggc gactctaacc
     6181 ctaccagcag ccgcgcgagg gtactttttc cgcagccgct gcgccccagc agggcgacgg
     6241 tttcaccgct tttcagggcc agggaaacgt tattcagcac cgcctggtgt tgatgtttgc
     6301 tgctaaatcc accgtgcgcg tagtgatggg aaaggtcgga gacgttaagt aaagtcatga
     6361 tgccagctcc ataccgtaga gggcgagatg agcggaaacc aggctgcgcg ttaccgcatg
     6421 tttgggggcg ttaaacagcg tttctacatc gccctgttca acaatcttac cgtgagacat
     6481 taccgccaca tcgtctgcca gacgcgctac cacgcccata tcatgggtaa ccagcagcat
     6541 tcccggtgct tgtttttgca taatgctttc cagcagatcg aggatgcgcg cctgtgctac
     6601 cacgtcgaga tcggtggtcg gttcatcggc gatgataaat ggtgattcgc acagcaccgc
     6661 catcgcaatc atcatgcgct gcaacatgcc gccgctcatc tcgaacgggt agagcttcag
     6721 cacgcgcgcg gcgttttcca gccccaccgc ttctatggca gcggtaagcg tagcgtcatc
     6781 ggcgggtttc cccagcgcca ggcaggtttc acgcgcgtgg gtgtgcatgg tgtgcagcgg
     6841 attaaaggca ctgcgcgggt tctgcatgat ggtggcaatt ttgattccgc gcagggcgca
     6901 gggagaaacc ggttttccat cggccaaaat ttcccccgcc gtctggcgaa cgccagcagg
     6961 cagaatgccc agcgtcgcgg cgcaggtcag cgacttcccg ctgccactac cgccgactaa
     7021 cgccagcaca cgcccacgtt gcagggttaa cgatacgccg tgcaccagcg gctgcgcggc
     7081 ctgtagcgcg atattacgga gttcaatctg ttgtggcatt agtgtgcgtg ctccgtcacc
     7141 agatgagggt ccagatgatc gcgcagtgcg tcacccacca ggttaaaggc catcacgctg
     7201 ataaacagcg ccagccccgg ccagaacatt tgcagcggct gggtccagat atactgacgt
     7261 gcatcgttaa tcatcacgcc ccattcggtg gtcggcgcgg tcacgccgag gccgaggaag
     7321 gacatccccg cgacgtgtag catcatatga ccgatatcca gcgtcgccag caccagcagc
     7381 gaggggatca ccgcacctgc cagatgatca acaaacaccc gcacatggcc cgcgccagaa
     7441 agccgtgatg ccagcacaaa ctcgcgctgg cgcagtgaaa tcaccagact gcgcaccatg
     7501 cgcgcgtacc atgcccagtg cgaaagggcg atagcgataa ttacgttggt caacccggtg
     7561 ccaagcacgc cgaccataaa gaacgacaga atcgaggttg ggaaggtcat aaacatatcc
     7621 gcgacgcgca tggtggcctg atcaacgcgc ccgccaatca gcccggcgct gccgccaata
     7681 atcagcccta atgtcagcac caacagcagg caggccatta ccgagccgag cgacacgcgg
     7741 gtcgctgcca tcagccgcga gaaaatatcg cgccctaaat gatcggtgcc cagccagtgc
     7801 tgcgcatccg gcgagagcag gcgcgacggc aaatcaatag cctgagggtc atacggcagc
     7861 caccactggc tggtgagcgc aatcagcgcc agcagggcga taatgatcag cgccaggcga
     7921 accgaccagc gggaggagag gaaaaagttc acgcgtgcgc tccttcatga cgacgaatac
     7981 gcgggtccag cgcggcgttg agcaaatcga caatcaaatt acagaccaca aaaaccacca
     8041 ccatcatcag cgtaaagcac tggatcaccg gatagtcacg gttaaaaatc gccgacaccg
     8101 catagcgccc gacgcccggc caggcaaaga tgttttcgat aatcatcgtc ccgccaatca
     8161 gttcgccaat gtgcatcccc acggcagtga tcatcggcag cgaggcattg cgcagaatat
     8221 ggcggcgttc ggtctgtttg tcgctcagac cacgcagtcg tgcccaggtg acgtgacgct
     8281 gaccggcgac gtccagcata ctggcgcgca gcaaacgcgc gttaatcgcc agcgacataa
     8341 aggcgatgga taccgcaggc aaaatgatgt gctgccagcc gccgtaaccc atcgcgggta
     8401 gccattgcag atacaccgaa aacgccatca ccagtaaaaa cgccagccag aagttaggca
     8461 tcgacacgcc gagaaacgcg atgaaacgga cggcgaaatc cggcagacga tcgcgatggc
     8521 gcgctgccca gataccgagc ggtacggagg tgagcagaat taataccagc gccgcacctg
     8581 caagttccag cgtggcgggc aggaaattca gcatatcgtc cagcaccggg cgttggctgg
     8641 cgaatgagat accaaagtca agatgcagcg ccttccacaa ccaggtgccg tactggacat
     8701 acagcggctg atccagcccc agcatggtgc gggtagaggc cagcatctcc ggcgtcggcg
     8761 gcaggttaga taaacgcaaa taatcaagcg ccgggtcgcc ggtgccgagg cgcagcatca
     8821 aaaaaatgat caccgaggcg gcgagcacca tcgggatcag cagcagaaag cggcgtaata
     8881 cataacgcaa cattaaggtt tcaccggttt aatctgttcg aacgggattt cggtggcgat
     8941 cggcgcgtag gggatgttac ccagttccgg ttttgaaacc accatcattg agatgtaact
     9001 gataggcaga taaaccgcct cgtcatgcag acgggtcaga atgtcgcgat acagcgcctg
     9061 acgttgcgtt tcgtcatggg tcgccagcac ttcgccgatc tctttatcaa tcagcggctt
     9121 gtcggctaaa ccttgctgtg cctggaagtc agcgtgcgac ggtacgcgca ttgaactcag
     9181 gaaggcgtgt ggatcgtatg gcgcgcccca ggtgcggtgg aaaatcatgc caaaacgacc
     9241 gtcgcgctga cgagcataga tactgctctc ttcttcgcca atcagcgaga catctgcgcc
     9301 aatctggcgc atatcagcct gaatgatttc cgccatcgat ttgcttaacg catcggtgcc
     9361 gatgaacgac agttcaatgc gcagcggctg accatttttc tcgcggatgt ctttgcctgc
     9421 aggcagcgtc caaccggctt tttccagcaa cgctttcgct ttctgcggat cgtactggcg
     9481 cggtttcagg ccgaggttgg cgtagggcac agaaggggca aacagggtgt cggcgacctg
     9541 ctgggtacca tacaacgcgt tatcaatcag cgattttttg ttcaccgcgt aattaagagc
     9601 ttcacgtact gccagctcgt tggtgggggc tttggcggta ttgagcgcca gcatcacggt
     9661 ttcgatcggc tgtgacagtt gagtgtggta agccgggttc tggctaaagc gggcgaaggt
     9721 atcgagcggt aataaccctt cgtttccgta cagcaggtcg atatcgccag tttcaaacgc
     9781 caccgcgcgg gtagtcgggt ccgggatcac gttaaaggtg atctttttaa tcgccggctt
     9841 ttcgccccag tagttttcgt tacggacgaa gacatcgtac tgattcagtt tcgattcctg
     9901 taaaacccac ggcccggtgc caatcggcgc tttaattccg ttcatggttt cgtggttttt
     9961 aaactgcgag ggtgcgataa aacggaaagg acggggcagg gccagttctt gcaggaaagg
    10021 atagtaggcg cttttcaggg tgatttgcag ctcgtttttg ccgagtgctt tgacatcaac
    10081 aatctggttt gccagctcca gccaggcgtg acgttgacgg ttatcgagca ctgcacggaa
    10141 gttttctgcc gccgcctctg catcaaacgg ttcgccgttg gagaacttca cgtcatcacg
    10201 cagggtgaag gtccaggttt taccatcttc tgaatgagtc cagctttttg ccagccacgg
    10261 gatcaccgaa ccgtctgcct gatatttcac cagcggttca taaaccatgc tctgggcgaa
    10321 catttggtta ggcgtgtaaa ggtgcgggtt tagtggcccg acattcaccg gccaggcggt
    10381 ggtgatttca tctggtgcag cggcatggac gataaaagac gcacaagcca gcagcgcaaa
    10441 tagagtgcgg cggagtgtag agaacatggt aaccccaatg gattaaaata gatggcggaa
    10501 ataagtatga cgattttaag tattcgtcat actgattacc tgttctggat caatagatgg
    10561 gcagtcgggt gggcggatca gttaactgaa tcgatccatt gcacactgtc ggcggtgagc
    10621 gtaaaggggg taggggtgca gatcgccagg cttaaatttt ctagctgaca atggctgact
    10681 gacagcgagg agttataggt gctgtctacg ctgacgattt gccaggcgct gccgccacgc
    10741 tgtttaacga tggcttcttt gcgcgtccag atgcgccaga acatttccag ttgctgatca
    10801 ggatgcacgg cgtccatctc ggcatgttcc ccaaggctga atacggcgtt cgccagccag
    10861 cgccagttgg cgcgcgggcg aatcacttcg atatcgcagc cgacttcgcc ttcatcgctc
    10921 aacagcaggg cgatatcgtc accactatgg cttaagttga accatagcgg cgtttccggc
    10981 gcgaatgcag gtttgccttg ttcgccatag atgatctccg gtagcgggga aagcgtgtgc
    11041 gaaagcaatg cacgccccgc cagccagcgt tcacgtcgtg gaccttgcgg tgcttgctcg
    11101 cgtaaacccg gtggcagagg agctgcgctt aaggtcgaaa ctttccccag aactatccga
    11161 tacatatcag ggccaacgtt taatggaaaa tgaaagtgcg tatcgtaaca cttgtcgcct
    11221 catcccggta accgactttt cggtctgcct ggccccagta aaatcgccag tttgctgccg
    11281 cctttggtgg tttccatcca gattttacac acgctggtta atggcaccga aagtagcatc
    11341 cctaccgggc cgagcagcca tccccaaatt aacaatgaaa gaaataccac catggtggac
    11401 atccccaggc gatggcccat catccgtggt tctaaaatat tgccgatgac catatggacc
    11461 actaaaaaca atgcgccgac cagaatacat tcgtaaatac cattaaacag cagcacctga
    11521 atcattggcg gtacggcgga aattaccgca ccgatattgg gcacgtagtt gagcaaaaac
    11581 gccagtactg cccacatcag cgcaaactgc acccccatca gctccagccc cagccagacg
    11641 attatgcctg tccataaact gagtagcgtc tttaacgcca gataatgcga aacgccttta
    11701 agtgcgcggt gtagtccggc gatgtgaatc tgtggattat tcaacgcgaa acgcattttg
    11761 taagggacgt ggcgcacttc aaacagcata aaaactacgg tcatcaccag caaaagcacg
    11821 ctcgccattg ccccggaaag cccggtcatt agcgccgtgg tgaaggtaac cactttttcc
    11881 gagtccatcc gctgtagtat tcgctccggc gacatatgca aattaagaaa aggcaacatc
    11941 tcctgcaatt taaaaagttt gcgcgtcagc tccttattaa acctcggcag catagagata
    12001 aattcgttaa acgatgccgc cagtacgccg accagcgcgg tgagtgcgat cagcatcacc
    12061 acaaccacaa tcgtaatggc aacggggcgt tgtacccccc gacgaataaa ccaggtgacg
    12121 agcgggttga gaacgatggc aaaaaacagc gccagtaata gctgcacaat gatatctgcc
    12181 gctgcgtgaa tgcccgcgag gatcactacc agcgaggcca acttgagcag aatgtgcatg
    12241 cccgttttat cgggttgagg ggtttccatt ggggcttcct tgtgactttt tgtattaagt
    12301 gtagcgggag tcacgccagc aatattctga ttcttactgc tgattttcac gtcagcacat
    12361 ggtaaaaatg aaacactgtt gtaaaaatgt ggtgatcctc atgcccgaac ccgtagccga
    12421 acccgcgcta aacggattgc gcctgaattt gcgcattgtc tccattgtca tgtttaactt
    12481 cgccagctac cttaccatcg ggttgccgct cgctgtatta ccgggctatg tccatgatgt
    12541 aatgggattt agtgcattct gggcaggggt ggttatcagc ctgcaatatt ttgccacctt
    12601 gctgagccgt cctcatgccg gacgttacgc cgatttactg ggacccaaaa agattgtcgt
    12661 cttcggttta tgcggctgct ttttgagcgg tttgggatat ctgacggcag gattaaccgc
    12721 cagtctgccc gtcatcagcc tgttattact gtgcctgggg cgcgtaatcc ttgggattgg
    12781 acaaagtttt gccggaacgg gatcgaccct gtggggtgtt ggcgtggttg gttcgctaca
    12841 tatcgggcgg gtgatttcgt ggaacggtat tgtcacttac ggggcgatgg cgatgggtgc
    12901 gccgttaggt gtcgtgtttt atcactgggg cggattgcag gcgttagcgt taatcattat
    12961 gggcgtggcg ctggtggcca ttttgttggc gatcccgcgt ccgacggtaa aagccagtaa
    13021 aggcaaaccg ctgccgtttc gtgcggtgct tgggcgcgtc tggctgtacg gtatggcgct
    13081 ggcactggct tccgccggat ttggcgttat cgccaccttt atcacgctgt tttatgacgc
    13141 taaaggttgg gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac
    13201 gcgtttgtta ttccctaacg gcattaaccg tatcggcggc ttaaacgtgg cgatgatttg
    13261 ctttagcgtt gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc
    13321 gaaaatcggc gtcttactgg cgggggcggg gttttcgctg gtgttccctg cattgggcgt
    13381 agtggcggta aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt
    13441 atttatggat ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagttgggc
    13501 gggcgttccg gtgatttatc tggcggcggc gggactggtt gcaatcgcgt tattactgac
    13561 gtggcgatta aaaaaacggc ccccggtgga aatacctgag gccgcctcat catcttaaaa
    13621 cttactgaat aaccagcgta ttaatgatgt tttctgcggt ggtctgtgct ttttgctgat
    13681 tgtcagcggg cagcgtaatt tgcatggtta gcagttgatt acccacgtta cccagaataa
    13741 cggaagagta cgccgtctgg cctttcgcgg agataatgct gtctaactgc tgcattttgt
    13801 gacctttcag ctcaatggct ttattggtta ccacttgcag ctgcggatcg cggctacgtt
    13861 gctgatcttc caggcgcttc gccagcaccg ccagatcttc tttcggatca tcgcccatga
    13921 tgacgatgac tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg
    13981 ttcccagctt accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat
    14041 caagcaggtt gacgggattc ccggtcgcgt tactttctgc gaccgaaccc tgcgccgtag
    14101 cgttagtgtc tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat
    14161 atttaaccag attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca
    14221 tccatcttat cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg
    14281 atttatctgc cagtcttttc aacagcatat tgagtaatac gccatacatt ggcaggaaga
    14341 aaacgatact gattaacact ttgaaacagt aatcgactag cgcgatttcc atccagtgtt
    14401 cagccataaa ggcatccggg ctacgccaga aggcaatgaa gaagaaggcc agcgtgtcgc
    14461 tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca ccagtgacga ctctgacgca
    14521 ggcggttaaa aacgtgcacg tcgaggatct gccccagcgc gtaggccatg aaactggcgg
    14581 tggcgatacg ggcgacaaac aggttgaagt gggcgagcgc gccgaatcct tgccatgacc
    14641 ccatataaaa cagcgatgag atgacgtagg agatcagcaa tgcggggatc attaccgcaa
    14701 agatgatgcg acgagccagc ggcgcgccaa aaatacgcac ggtcaggtca gtagcgagaa
    14761 agataaacgg aaagctaaac gcgccccagg tggtatgaaa accaaaaatg gagaccggaa
    14821 gctgaaccag atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca
    14881 acgccttata gcgttgcgtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat
    14941 tggggtgagg gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctga
    15001 cccttttttc gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc
    15061 acgcaatcgt taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt
    15121 tatgtgatga gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac
    15181 ttggcctgcg ctgcccagaa ccagtaatga tggtgcgcaa aaccgtgcgc aatatgcagc
    15241 ctggcgaaac gttgttgatt attgccgacg atccggcgac gacccgcgat attcctgggt
    15301 tttgtacctt tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt
    15361 atttgattcg taaaggcggt tgatggggga agatgggctt cgatactgcc ttttgccctc
    15421 accctaaccc tctccccaga gaggcgaggg gaccgatcgc actcaatgtt gctgcttgaa
    15481 accggtttgt aggccggata aggcgttcaa gctgcatctg gcgatacggt gcacaatgcc
    15541 tgatgcgacg ctgtcgcgtc ttatcatgcc tacgagttcg atgctctgtg taggccggat
    15601 aaggtgttca cgccgcatcc ggcattgttg gaccgtagac tgatagcctc tttatttcct
    15661 gcgcaataac cgtaacgcat tcgccgtcac cagcaccgtc gcacccgtat ctgccagcac
    15721 tgccagccat agtccggtca tccctaacag cgtggtgacg aggaagatcc ctttcagccc
    15781 cagcgcaatg gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca gttcaatcat
    15841 ttgcaccagg ccgcgcaggt ggttatgggt taatgctgcg tcggcggttt ccagggccac
    15901 gtctgtgcca ctgcccattg caatcccaat agcggcggct ttcatcgctg gcgcgtcgtt
    15961 aataccgtcg ccgaccatcg ccagcggcgc atgttgattc agctcggtca ccgctttaac
    16021 tttatcttcc ggcaagaggc ccgctttaaa ttccagcccc agttccccgg cgattgccgc
    16081 tgctgcgcgt ggattatcgc ctgtaaggat cacccctttg acgcccagcg cgttcagttc
    16141 actgatagca gttgcagcat cggcgcgcag ggtatcctgc aacgcaatga cacccagcac
    16201 gtcatcatta catactacca gcactaccgt ttgcccggcg ctttccagtt cattaatcaa
    16261 accagcaaat gcatcagcag gatgttttcc tgcagcgcat atcaacacgc gctcaccgtt
    16321 aacctgcgct tcaatgccag acccgaccag cgcccgctgt gattgggcgg tgggaatggc
    16381 gagttcagca acctgtgctt cgcgcacaat ggcctgtgcc agtggatgcg tcgcgccttg
    16441 ctcgaccgcc gccgccagtg tcagcagttc agattcacta atacccgttg ccggatgaat
    16501 cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg ccacctgggt
    16561 aacacgaccc agctgctcca gcgccgcgcc gcctttaatc aacgccccac gacgcgctgc
    16621 tgccgccaac ccggaggtaa tcgccgcggg cgtggagata actaaagcac acgggcaacc
    16681 aatcagcagc agcgtcagcc ctttataaat ccactcctgc cagctggcgg caaacagcag
    16741 cggcggcacc agcgttacca gcagggcgac ggccataatc gctggcgtgt aaatacggct
    16801 gaaacggtcg ataaaccgct caataggtgc gcggcgctct tcggcttctt caatcagttt
    16861 cagaatccgg tcaatggcgc tggcacccgg ttctgacagc acttccagcg ttaccagacg
    16921 gtctacgctg gtggtgcctg caggaacttt atcgcccgtc gcgcgctcca caggaatgga
    16981 ttctccggtc agggcacttt catcaaaact ggcaaacggt gagagcagtt taccgtcggc
    17041 aggcaaacgc ccacctgccg caacttcaat cacatcgcca ggccgcaggc tgtttattgc
    17101 cacctcttca cgctcaccgt tacgcagacg cgtggcggtt tctggcttca gcgccattaa
    17161 cgcgctgacc ccttgacgcg cgcgactggc ggcccagcct tccagtcgtt caccaatcaa
    17221 aaacagcagc aacaccatcg cagcttcagc cgttgcgcca ataaacagcg caccaatagc
    17281 ggctacgctc attaaggttt caatggcgaa gtagctgcca gatttgatca gccgtaatgc
    17341 ctgacgagca attgggtaca gcccaaccag cgtggtcgcg ataaacgcca gttgaccaaa
    17401 cggatgattg aactgctcca gcccccaact gattgccatc atcacgataa gcgtaatcag
    17461 cggcagattc tctttcaggc gtgattcttg tggttcgtcg gaggcctgtt catcgcgcag
    17521 ggaatagccc gctttttgca ccgcagattc aacttgtgcc cggatgtcat tgtcggcatc
    17581 caccaccagt ttttcggtgg cgaacaacac ctgtacctga ttcacgcctg caagctggcg
    17641 cacggcattt tccactttgc gcgcacaggc ggcgcagtcc ataccgctga ctttccagct
    17701 atagcgggtg ccggagacgt tttcagagag agttggcgtg ctggaacatg cgccgccgca
    17761 gcaacagtcg ttggtgttct gtaccgtagt tagcggtttg aacgcagcaa attgaggggc
    17821 tttcttgccg tgattgtcag gagtcgacat ggcatcctcc ggttaagttt tttctcatta
    17881 accgaaggat acactctgga gtcgactcca gagtcaagtt ttatcagaga tacagcgagc
    17941 ggacgatgag gaagtgacca gcaaagtagc aggccgcggc aatggcgtta tccgcacgga
    18001 agcgtcggcg atagtgacta cccagccaga caaagttact gataaacagc aacgaagcgc
    18061 cgacaaacgc agagagcgcc ggagcggtcg gacggaagaa ccacagttca cctgccagcc
    18121 acaccatcac cagcgtcatg ccgataaacg tgcagatagg ccaacgatac tcttccagac
    18181 gcgtccagat aatcgccagt aacagcgcgc ccagaaccag cagcaccaac ggcagaggcc
    18241 agaagaaaga gagcgtcatc tgactggcga aatagatggt gtacagcagg tgcgagagga
    18301 aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa cagggttagc gcatcgccca
    18361 gcagtgaggc acacagcccc gccagcacca gatagctaat ggcgtcgaac atcggcgctt
    18421 gccaggccag caacagcagg agaagaaggg ttaacggttt aaacacccag cgttgccagg
    18481 ttggcccacg atacgatgca tccacagata gccatgcgga aagacagaca gcgataaacg
    18541 accaaagcat cttagttcct tgaattcgtt attttttcgt aagcggaatg cctacggtct
    18601 tgcttccagt ttagtggcga gggtggacgg atgacaacac cataactggc agggtatgct
    18661 tatttccgca ttttccgatg agggataaag atgagtaaac cacctctttt ctttattgtt
    18721 atcattggct taatcatcgt ctccgcatcg tttcgtttta tgcaacaacg acgggaaaaa
    18781 gctgataatg atatggcacc gctccagcaa aagctggtgg tggtgagcaa caagcgggaa
    18841 aaaccgatta acgatcgccg ttcgcgccag caggaagtga ccccggcagg caccagtatg
    18901 cgctatgagg caagcttcaa accgcaaagc ggaggaatgg agcagacgtt tcgcctcgac
    18961 gcccagcagt accacgccct gacagtgggt gataaaggta cgctgagcta taaaggaacg
    19021 cgctttgtca gctttgtagg cgaacaataa ggttattttt taactttaaa tttcttctgc
    19081 cagaccagca gttcaaacac gccgaaaagg aaaatccgca ccttttcggc ggtggtcatc
    19141 tgcgggccat ctttcggtaa agtggatttc aatagcgcca gctgcatacc atgcatcagc
    19201 acggtaaaaa tcagcgccac gttaacgaaa atattcagcg ggcgtgggaa gggatgcacc
    19261 aggttgagga ttaaaaatcc ccaaacacag agcattaaca aacgaccaat attaatcagc
    19321 atcactttct ccttgtgctt cgcgttgata cagccgataa gccacctgac ccgccacttt
    19381 ttcccgatgt aatgaccagt ttgccggaac agtgggcaga ccgttttcga cttcgctttc
    19441 gacataaatc agggcttcgt cagccagcca gccgttatct tccagtaaat ttatcgtctc
    19501 ttctaacaag ccacggcgga acggtggatc gacaaacacg atattatgcg gtgtgccttt
    19561 ttgcgccagg aatgacatcg cgttgctgtt caccacgcgt gcattgcctg cttttagtgt
    19621 cgccagattc ttaattaact gctgagaaac cgcacgatcc atctcaatca acgtggcccc
    19681 cgcagcgtag cgcgataacg cttccagccc cagcgcgccg ctcccggcga agcaatccag
    19741 acattgggcg tcaacaatga ccggagccag ccagttaaac aacgtttcgc gtacgcggtc
    19801 ggtggtgggg cgcagacctg gactatcagg aaccgggagt ttacggcctc gccactgccc
    19861 gccaataatg cggatttggc cgctgccgga atgattcggt tttttcatga taaattgctc
    19921 aatccgccag ataacacata cttgcaggcg gtgatgtgaa ttaagttaag tgatagacta
    19981 tttcatcatt tttttagctg ctatgtacat agcgttaacg ctgtgccatg aagcaacagc
    20041 gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc tggctgggct
    20101 ttggtcaaaa agagcagatc ccggaaaaag agacagaagt tcagaatgaa caaccagttg
    20161 tagaagaaat cgttcaggcg caagagcctg tgaaggcttc tgaacacgcc gttgaagagc
    20221 agccgcaggc gcatactgaa gccgaggcgg aaacttttgc tgccgacgtt gtggaagtta
    20281 ctgaacaggt tgctgaaagt gaaaaagcgc agcctgaagc ggaagtcgtt gcacagccgg
    20341 aaccggtcgt agaagaaacg ccggagccag tggctatcga acgtgaagaa ctgccgttgc
    20401 cggaagacgt caacgccgaa gcggtttcgc cagaagagtg gcaggccgaa gcggaaaccg
    20461 tagagattgt cgaagcggcg gaagaagaag cggctaaaga agaaatcacc gacgaagagc
    20521 cggaggcaca ggcgctggct gccgaagcgg cagaagaggc ggtaatggtg gtttctccgg
    20581 cagaagaaga gcagccggtg gaagaaatcg ctcaggagca ggaaaaaccg accaaagaag
    20641 gtttcttcgc gcgcctgaaa cgcagcctgt taaaaaccaa agaaaatctc ggttccggat
    20701 ttatcagtct gttccgcggt aaaaaaatcg acgatgatct gtttgaagag ctggaagaac
    20761 agctgttgat cgccgatgtg ggcgtggaaa ccacacgtaa aattatcacc aatctgacgg
    20821 aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct ctatggcctg ctgaaagaag
    20881 agatgggcga gattctggcg aaagtcgatg agccgctgaa tgttgaaggt aaaacgccgt
    20941 tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac cacgacgatt ggtaagctgg
    21001 cgcgtcagtt tgagcagcag ggtaaatcgg tgatgctggc ggcaggcgat actttccgtg
    21061 cggcagcggt tgaacagctt caggtctggg gtcagcgcaa caatattccg gtgattgccc
    21121 agcataccgg tgctgattcc gcttctgtta tcttcgacgc cattcaggcc gctaaagcgc
    21181 gtaacatcga cgtcctgatt gccgataccg ccggacgcct gcaaaacaaa tcgcacctga
    21241 tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa gctccgcatg
    21301 aagttatgct gactattgat gccagcaccg ggcagaatgc ggtaagccag gccaaactgt
    21361 tccacgaagc cgttggctta accggcatca cgctaacgaa actggacggc acggcgaaag
    21421 gcggggtaat tttctcggtg gctgaccagt ttggtatccc tatccgctac attggtgtcg
    21481 gcgaacgtat tgaggatttg cgtccgttta aggcggacga ctttatagag gcactttttg
    21541 cccgagagga ttaacaatga ttcgctttga acatgtcagc aaggcttatc tcggtgggag
    21601 acaggcgctg cagggcgtta cgttccatat gcagccgggt gagatggcgt ttctgaccgg
    21661 tcattccggc gcagggaaaa gtaccctcct gaagctgatc tgtgggattg agcggcccag
    21721 cgccgggaaa atctggttta gcggccatga catcacgcgt ctgaaaaacc gtgaagttcc
    21781 gtttctgcgc cgccagattg gcatgatttt ccaggatcac catctactga tggaccgtac
    21841 tgtctatgat aacgtggcga tcccgctgat tatcgccggt gccagcggtg atgacattcg
    21901 tcgccgggtg tcggcggcgc tggataaagt cgggctactg gacaaagcga agaacttccc
    21961 tattcagctt tccggcggtg aacaacagcg tgttggcatt gcccgcgcgg tggtgaacaa
    22021 gcccgcggta ctgctggcgg acgaaccgac tggtaacctg gacgacgcgc tgtcggaagg
    22081 cattttacgt ctgtttgaag agtttaaccg cgttggagta accgtattga tggcaacgca
    22141 cgacatcaac ctgatttcgc gacgttccta tcgcatgctc accctgagcg atggtcactt
    22201 gcatggaggc gtgggccatg aataagcgcg atgcaatcaa tcacattcgg cagtttggcg
    22261 ggcgtctgga tcgcttccgt aaatcggtcg gcggctctgg cgacggcggt cgtaacgcgc
    22321 cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt ttcaacgaac
    22381 aggtacgcta tgccttccac ggcgcattgc aggatctgaa aagcaaaccg ttcgccacgt
    22441 ttttaacggt gatggttatc gccatctccc tgacgctgcc tagcgtctgt tatatggtgt
    22501 acaaaaacgt taaccaggcg gcgacgcaat attatccgtc accgcaaatc actgtttatc
    22561 tgcaaaaaac gctggatgat gacgccgctg cgggcgtggt ggcacagttg caggccgagc
    22621 aaggcgtgga gaaagtgaat tatctttctc gtgaagacgc actgggcgag taccgtaact
    22681 ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa cccgcttccg gcagtagcgg
    22741 tggtgatccc gaaactcgat tttcagggga cggagtcact gaatacgctg cgtgatcgta
    22801 tcacgcagat taacggcatt gacgaagtgc ggatggatga cagctggttt gcccgcctgg
    22861 cggcgttaac cgggctggtc gggcgcgttt ccgcgatgat cggcgtgttg atggtggcag
    22921 cagtgtttct cgtcatcggc aacagtgtgc gtctgagcat ctttgctcgc cgtgactcta
    22981 ttaacgtgca gaaactgatt ggtgcgacgg atggtttcat cctgcgcccg ttcctgtacg
    23041 gtggcgcact gctgggattt tctggcgcat tgttgtcatt aattttgtca gaaattctgg
    23101 tgctgcgatt gtcatcggcg gttgcggaag tggcacaggt tttcggaacg aagtttgata
    23161 tcaatggttt atcgttcgat gaatgcctgc tattgctgct ggtatgctcg atgattggct
    23221 gggtggcagc gtggcttgcc acggtacaac atttacgcca ctttacgcct gaataataaa
    23281 agcgtgttat actctttccc tgcaatgggt tccgtagcag ggaaagagac cccgttgtct
    23341 cttcccggta tttcatctct atgtcacatt ttgtgcgtaa tttattcaca gcgttgcatt
    23401 gaacttgtgg ataaaatcac ggtctgataa aacagtgaat gataacctcg ttgctcttaa
    23461 gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga gaggatttga
    23521 atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga ttcctacatc
    23581 cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct ggctgaaaag
    23641 ctgcattacc atggcgatct ggaagcagct aaaacgctga tcctgtctca cctgcggttt
    23701 gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga tttgattcag
    23761 gaaggtaaca tcggcctgat gaaagcagtg cgccgtttta acccggaagt gggtgtgcgc
    23821 ctggtctcct tcgccgttca ctggatcaaa gcagagatcc acgaatatgt cctgcgtaac
    23881 tggcgtatcg tcaaagttgc gaccaccaaa gcgcaacgca aactgttctt caacctgcgt
    23941 aaaactaagc agcgtctggg ctggtttaac caggatgaag tcgaaatggt ggcccgtgaa
    24001 ctgggcgtaa ccagcaaaga cgtacgtgag atggaatcgc gtatggcggc acaggacatg
    24061 acctttgacc tgtcttccga cgacgattcc gacagccagc cgatggctcc ggtgctctat
    24121 ctgcaggata aatcatctaa ctttgccgac ggcatcgaag atgataactg ggaagagcag
    24181 gcggcaaacc gtctgaccga cgcgatgcaa ggtctggacg agcgtagcca ggatatcatc
    24241 cgcgcgcgct ggctggacga agacaacaag tccacgttgc aggagctggc tgaccgttac
    24301 ggcgtttccg ctgagcgtgt gcgtcagctg gaaaagaacg cgatgaaaaa attgcgtgct
    24361 gccattgaag cgtaatttcc gctattaagc agagaacctt ggatgagagt ccggggtttt
    24421 tgttttttgg gcctctgtaa taatcaattt cccctccggc aaaacgccaa tccccactca
    24481 gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatcggg tatgttttag
    24541 cagagtatgc tgctaaagca cgggtagtca tgcataaaac gaaataaagt gctgaacaac
    24601 aacatcacaa cacacgtaat aaccagaaga atggggattc tcaggatgaa catgaagggt
    24661 aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct ggcagaagat
    24721 attaaagtcg cggtcgtggg cgcaatgtcc ggtccggtgg cccagtacgg tgaccaggag
    24781 ttcactggcg cagagcaggc agtagcggat atcaatgcta aaggtggtat caaaggcaac
    24841 aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt tgcggtggcg
    24901 aacaaagtcg ttaacgacgg cattaaatac gtgattggtc acctctgctc ttcatcaacg
    24961 cagccagcgt ctgacatcta cgaagacgaa ggcattttaa tgatcacccc agcggcaacc
    25021 gcaccagagc tgaccgcccg tggctatcag ctgatcctgc gcaccaccgg cctggactcc
    25081 gaccaggggc cgacggcggc gaaatatata cttgagaaag tgaaaccgca acgcattgct
    25141 atcgttcacg acaaacagca gtacggcgaa ggtctggcgc gtgcagtgca ggacggcctg
    25201 aagaaaggca atgctaacgt ggtgttcttt gatggcatca ccgccgggga aaaagatttc
    25261 tctaccctgg tggcgcgttt gaagaaagag aatatcgatt tcgtttacta cggcggttat
    25321 cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg cagggctgaa aactcagttt
    25381 atggggccag aaggcgtggc taacgtctcg ctgtctaaca ttgcgggcga atcagcggaa
    25441 gggctgctgg tgaccaagcc gaagaactac gatcaggttc cggcgaacaa acccattgtt
    25501 gacgcgatca aagcgaaaaa acaggaccca agcggcgcgt tcgtctggac cacctacgcc
    25561 gcgctgcaat ctttgcaagc ggggctgaat cagtctgacg atccggcgga gatcgctaaa
    25621 tacctgaaag cgaactcggt tgatacggtg atggggccgc tgacctggga tgagaaaggc
    25681 gatctgaaag gctttgagtt cggtgtgttt gactggcacg ccaacggcac ggcgactgat
    25741 gcgaagtaat cattaatcgg caactttggg ttgccgccaa attgctaata tcgagtacgt
    25801 tgcttcatgc cggatgcggc gtaaacgcct tatccggcct acaagattca aagaaatcag
    25861 taaattgcag cacacattgt aggcctgata agcgtagcgc atcaggcaat atcacttttg
    25921 aaatcaaact tgacgattaa tgcttctccc agccaccctg ttgtgccgta aatcccagtg
    25981 ctcgcataaa cgccgtcatc acgccgcgat cttccacgcc agcatccgcc atccaccaac
    26041 atgaaacgtc aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac
    26101 gtcgggtgac ttcccgcacg cgtagagaat ccagtgcccc ctcggtgccg cttaaggtta
    26161 cccgcacggc agcgagcagg cgctcgttaa aacgcgcggc gtagatacgg tagttatcgt
    26221 caacctgtaa agaggaaggg gaatattccg gccagatctt ttgcaggtca atccggtcct
    26281 ggtcgctaaa gttttctaat cgaatgatgg tcagcttcat ggggaacccg tgtaaatcac
    26341 aaaagtggag ccagtgtagc gaaataattt aatcggagg
//
LOCUS       DAZGJT010000066        26312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZGJT010000066 DAZGJT010000000
VERSION     DAZGJT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.066"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..342
                     /gene="yhcN"
                     /locus_tag="ACHKFK_002979"
     CDS             79..342
                     /gene="yhcN"
                     /locus_tag="ACHKFK_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO9053267.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(397..669)
                     /gene="yhcO"
                     /locus_tag="ACHKFK_002980"
     CDS             complement(397..669)
                     /gene="yhcO"
                     /locus_tag="ACHKFK_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO9053268.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(761..2728)
                     /gene="aaeB"
                     /locus_tag="ACHKFK_002981"
     CDS             complement(761..2728)
                     /gene="aaeB"
                     /locus_tag="ACHKFK_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO9053269.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATCYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFLGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLTGMLHKYQHALTDS"
     gene            complement(2734..3666)
                     /gene="aaeA"
                     /locus_tag="ACHKFK_002982"
     CDS             complement(2734..3666)
                     /gene="aaeA"
                     /locus_tag="ACHKFK_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO9053270.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            complement(3674..3877)
                     /gene="aaeX"
                     /locus_tag="ACHKFK_002983"
     CDS             complement(3674..3877)
                     /gene="aaeX"
                     /locus_tag="ACHKFK_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO9053271.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            4060..4989
                     /gene="aaeR"
                     /locus_tag="ACHKFK_002984"
     CDS             4060..4989
                     /gene="aaeR"
                     /locus_tag="ACHKFK_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO9053272.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(5117..6562)
                     /gene="tldD"
                     /locus_tag="ACHKFK_002985"
     CDS             complement(5117..6562)
                     /gene="tldD"
                     /locus_tag="ACHKFK_002985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO9053273.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTIVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(6718..10518)
                     /gene="yhdP"
                     /locus_tag="ACHKFK_002986"
     CDS             complement(6718..10518)
                     /gene="yhdP"
                     /locus_tag="ACHKFK_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO9053274.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGVRPLVAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPVAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWPALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTQSSPFPTTERINFRGWPDAQIRCAECWFWGQKFGHIDSDITIFGDTLTLTNGLI
                     DTGFARLTAEGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(10586..12055)
                     /gene="rng"
                     /locus_tag="ACHKFK_002987"
     CDS             complement(10586..12055)
                     /gene="rng"
                     /locus_tag="ACHKFK_002987"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO9053275.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(12045..12638)
                     /gene="yhdE"
                     /locus_tag="ACHKFK_002988"
     CDS             complement(12045..12638)
                     /gene="yhdE"
                     /locus_tag="ACHKFK_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO9053276.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADRQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSFHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(12647..13135)
                     /gene="mreD"
                     /locus_tag="ACHKFK_002989"
     CDS             complement(12647..13135)
                     /gene="mreD"
                     /locus_tag="ACHKFK_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO9053277.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(13135..14238)
                     /gene="mreC"
                     /locus_tag="ACHKFK_002990"
     CDS             complement(13135..14238)
                     /gene="mreC"
                     /locus_tag="ACHKFK_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO9053278.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGITQPTPQQPATGNAVTA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(14304..15347)
                     /gene="mreB"
                     /locus_tag="ACHKFK_002991"
     CDS             complement(14304..15347)
                     /gene="mreB"
                     /locus_tag="ACHKFK_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO9053279.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(15652..17592)
                     /gene="csrD"
                     /locus_tag="ACHKFK_002992"
     CDS             complement(15652..17592)
                     /gene="csrD"
                     /locus_tag="ACHKFK_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO9053280.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTND
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLCEIQNAREQHSRLDT
                     LIRSYAAQDMKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFSLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMLHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGRVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLLISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRRRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            17744..18718
                     /gene="yhdH"
                     /locus_tag="ACHKFK_002993"
     CDS             17744..18718
                     /gene="yhdH"
                     /locus_tag="ACHKFK_002993"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO9053281.1"
                     /translation="MQALLLEQQDDKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPARRA
                     QAWQRLVADLPESFYTQAAKEISLAEAPKFAEAIINNQIQGRTLVKVN"
     gene            18906..19293
                     /locus_tag="ACHKFK_002994"
                     /pseudo
     CDS             18906..19293
                     /locus_tag="ACHKFK_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240072.1"
                     /note="in Escherichia coli this inner membrane protein was
                     found to anchor the periplasmic catalytic oxidoreductase
                     YedY; sulfite oxidase activity not demonstrated; contains
                     heme; frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoxide reductase heme-binding subunit YedZ"
     gene            19698..20168
                     /gene="accB"
                     /locus_tag="ACHKFK_002995"
     CDS             19698..20168
                     /gene="accB"
                     /locus_tag="ACHKFK_002995"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO9053282.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            20179..21528
                     /gene="accC"
                     /locus_tag="ACHKFK_002996"
     CDS             20179..21528
                     /gene="accC"
                     /locus_tag="ACHKFK_002996"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO9053283.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRSDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(21620..22666)
                     /locus_tag="ACHKFK_002997"
     CDS             complement(21620..22666)
                     /locus_tag="ACHKFK_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO9053284.1"
                     /translation="MKNEIWSWIGDLSQVAGIKHYELRSGRAKGTEAFDVRTGAGLAF
                     TVVKDRALDIAWASYKDTALSFITPNGVVAPAFFESQGNGFLRSFYAGLLTTCGLSYI
                     GTPCEDEGETLGLHGRLAATPAEEVGYRTERTDDSIEFVINGKVRETRLFGENLTLER
                     TIRCRYGENVMRIEDKVTNHGFTRQPLQILYHFNYGWPLLSPQAEILLSAKSVTPRTL
                     HAAEGLASHLEICTPQPGFDEQVYYLTLNSDSQGMSKVALVNAELGWGIYEKFDTMQL
                     PNFIQWKNLGAGEYVMGLEVSNSFPDGRDKERAQGRLPFIEPGETKKYCFELGIVDGD
                     AEISALKAEIAGYR"
     gene            complement(22663..23625)
                     /locus_tag="ACHKFK_002998"
     CDS             complement(22663..23625)
                     /locus_tag="ACHKFK_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9053285.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKENGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDAHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIETFIQRHDMSIREAAQ"
     gene            complement(23647..24636)
                     /locus_tag="ACHKFK_002999"
     CDS             complement(23647..24636)
                     /locus_tag="ACHKFK_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9053286.1"
                     /translation="MTEKTLASPKPSEIPIANNFKKVFRQYGGILSGMVVLIILFSFI
                     NDSFFTANNITNIILQVSIIAITAYGMTYVLLLGDIDLSVGSTIALIGTFAALGASWG
                     IPFVLLVPLSIIAALALGMINGGLTAIAGIPSFIVTVATMGIFRGIAYIVTDGMPIMI
                     KDDAFLALGNGEFLYIPIPIWILIILLLINHFILTKTTFGRKIYITGGNKEAAIYSGI
                     NVTRLKIKVFMITAVLAGINGMILASRLYSGQPNSALSYELDAIAAAVLGGTSLNGGY
                     GTVVGTVIGALTIGVINNGMNLMNVPYFYQMVVKGLVILVAVYFDVRNKRKRS"
     gene            complement(24637..26136)
                     /locus_tag="ACHKFK_003000"
     CDS             complement(24637..26136)
                     /locus_tag="ACHKFK_003000"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO9053287.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAELGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEKPDENVPAKLEVKNLSHPSLFKEV
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYHGTVLIDGKEVRIANPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
BASE COUNT         6578 a         7092 c         6302 g         6340 t
ORIGIN      
        1 taattagtgt atacttgatt ttgtgatatg ggtcacgaaa caaaggccca gctaaaagat
       61 tatgtcgagg taaaaatcat gaaaatcaaa accactgttg ctgcattaag cgtactttct
      121 gttctctctt tcggtgcatt cgctgccgac tccattgatg ctgcacaagc acaaaatcgt
      181 gaagcaatcg ggaccgtatc cgtaagtggt gtggcgtctt cgccaatgga tatgcgtgaa
      241 atgctgaaca aaaaagcgga agagaaaggc gcaacggcct accagattac tgaagctcgt
      301 agtggtgaca cctggcacgc tacggctgaa ctgtacaaat aaaccctcat cgtcttgtcc
      361 gacgatattg cccccggttc gggggctttt tttgcgctaa tgacgaacat taaaacgcaa
      421 atgcccttcc agctcttcct ctgcctcatc aaacagcaat attaacgcgc caaaacggcg
      481 acgcgttttc tctcccagat gaacaaattc aatctcaagt ggtagcggca ggacatcgtt
      541 cattaacaca tcccatagtg agtcgagatc gcgtacctta tctttcgcca gaccaaaggt
      601 ttggctaaag tcacgataaa aatcctcctg actctcaatc tcatcaaaat caaaggtata
      661 aatattcatc tgttgccacc gtcacgtttc gcggcgagtt ctgccgttca tcgctaagta
      721 tagccatgaa aaaaccgacg cttcgggcgt cggttttgac ttaactatcg gtcaacgcat
      781 gttgatactt atggagcata cccgtaagcc gatgtactgg ttccgtcacc tgcggtggcg
      841 cttgccagat gcgtagcttt tcctggtaga tttccagttc ttccagcaac tggccaaagt
      901 accgacgacg tttatcatcg ctacgggcag atatcacatg gtctgctgtg cggcgcattt
      961 gccggtgaaa cgccgataaa tcctcgttaa ccggaatcgg cgcatcacgc aggcgctggt
     1021 gcgcgataat catcgtcagc gccagacgaa atttcggcaa atcccctagg aacttattca
     1081 tcagcaaaaa cagctgctga taaagtgccg gaaggtgatt ctctttacga cgtgccacat
     1141 tggtagtcat cgcggaaaca gcggcagaaa caaactgatt aagcagtaca cgtccagtcc
     1201 tgtcgcgcga tttatcccgc accagcaaaa taacggtgaa cgcgagcaca cagccgacga
     1261 tttgccctaa tgcgctgtcg agaaattgac tgaaatggaa agtcatcggg ttatccagca
     1321 cgataatgtt tatggtgctg gccaacgccc ccatcgagcc caatctccgt ttctgtactt
     1381 ctataccgag gaaaaatccc agcactgcca ggctaataca cagcagcaac atgctctgtt
     1441 gggtattagg gataatcacc aaaaagtaga gcagccctaa cggtagcgct gccagcgtcc
     1501 cgtagataaa gtcgatcgcc accatgcgtg gattcggcaa acgcattgcc agtgacgtca
     1561 ctaccgcaat catcaccatt gcaccactgc cggaagtcca gcccgtccac agccagaaaa
     1621 gcgtgcccag aatgcaagaa agtgtggttc gccagaagtt aaccattgca tgatgacgtt
     1681 cggctgactc tacttttact tccggttcgc cttgcaggat ctcttcttcg gtggcgttga
     1741 tttttgtgtt actgataacg ccgcgcttga gaagctgata acacgttgcc gccgcgaccc
     1801 agctataaat ggtgacaggc gtttcccgtt ccccggtcca ggcgatcact ctccgcaggc
     1861 gtttgagctg cttgtggacg tcctgcgcag tttctaccgg tgtgtcaaaa aattcgcgga
     1921 aagtatcagt gatcaattcc gggcgcgtat tctgaataag ataagtttcg caggattggg
     1981 taatcagcgt cagcgatagc gtattgatcg cttttaaacg tcgattggcc cgcgcccagc
     2041 gggaagattc catattcagg ttgctgcgca taccttgtaa cgccgtagta cgtcgcacca
     2101 gatcgcccca ggctttatcg acaacttcac catcgccatg cttgatacag agttgcatta
     2161 attgatattg cgcaaccagc aaactttcca gctctcgatc cacttcttgt ttgatcgatc
     2221 gcggagaaaa gagcaaatcc gccataattg cacacacaat accgataacg atctcgctac
     2281 aacgttcgac ggcaaactgc ggcgtaagca atggttccgg ctgaatggtg atcacaatga
     2341 tcagcgcggt ataaccggcc agcccccacg catacgagtt ttctattcgt accagcgagg
     2401 atatccaggt acaaaaaccg gcccagatac agcacaccag aatcatcaat aatggtgctc
     2461 ggatcatcgc aatgatgatt accagtccgg caatacagcc aataaatgtg ccgatgatgc
     2521 gcaaaaagcc acgatagcga atagcgccag aatacggttc acctcccgca gcaaaggccg
     2581 gaccggcggc aacaatcgcc gctgtcagta ccgcccagcg tggcgtttcc agctggaagt
     2641 gaaagccaac aaacagcgcc agtacaatgg cggtcgccag ttttaccgca aagcgaatat
     2701 gttgattagc aatggagaaa atacccatcg tgattaacca aactcacgca ggcgatgagc
     2761 cattttacgg aagaacgaat cctgactttc gtcgcgatct tgtttgccag tgactaccac
     2821 cgtagcagtg gtgcccgcag gccagatgtt ctcttgctgg ttgtcgagac gaatacgtac
     2881 cggaacacgt tgcgccaaac gtacccattc aaggttagag tcgatggtcg ccatcccttt
     2941 gtcgtcacgc gtgctgctgg cattggtgac ccctgcggca acactatcaa cagtcccttt
     3001 cagcactttg ttactgccaa gcggcgtgat ctccgcacga tacccagggc gcaccccttc
     3061 cagcttagtt tcttccatat aggccagtac atagaaggag ttctgtttca ccagcgcaac
     3121 cgccgttgat cctcgagtaa taaactcacc ggtatagacg ttgaggttgg tcacccagcc
     3181 atctgctggc gcgcggatga ctgtacgttc aagatccagt tttgccagat cgcgggtcgc
     3241 ctgcgctttc gctaactgat gcagaactgt ttgtagtacg ttgttcgcct ggtcgatctc
     3301 ttcgcgagac atcgcctgca caccgagacg gttacgacgt ccggcctcct ggcgtttctc
     3361 ctgtgccagt acctgataat aagcaacatc ggcttgcgct tcctcaagcg ccttttgata
     3421 gcgcggctgg tcgatggtga acagtacctg tccttttttc accagctggt tatcatgaac
     3481 attcacctgg gtaatgagtc cagaaacgtc cggcgcgatc gcaacgacgt cagcgctaaa
     3541 gcgcgcgtca cgcgtccacg gggattcggt gtaatagacc caggcattaa aaattgcgat
     3601 gaaggccaga atgactaata cgaccgtgat ggccgtacgg gagaattttc ttattagtgt
     3661 tttcacttca acctcaaacg aacagtcgcg atatcaaata aaacaagcag caatagagcg
     3721 cggtgttgaa caacgccgga tgccagacaa agtcgtagat acctgttggc acaagtaccc
     3781 ggcgcaccag ccagaaaatc gccagtgata aaagcaattc gaaaaatatc ggtgggaagg
     3841 acagcccaaa caccacgata acgggaaaca gactcatgtt gaccttggtt gtatagagag
     3901 agcaggcgtt attattttca gcatctgtcg ccgcagagaa gggcatggaa agccgggcga
     3961 gagcaacatt gctgtagatt gatatttaat atattaacgt aactgttatg ctgttatcta
     4021 tattatgtga tctaaatcac ttttaagtca gagtgaataa tggaacgact aaaacgcatg
     4081 tcggtgtttg ccaaagtagt tgaatttggc tcttttaccg ccgccgccag acagctacag
     4141 atgagcgttt cgtccatcag tcagacggtg tcaaaactgg aagatgagtt gcaggtaaag
     4201 ctgttaaacc gtagcacacg cagcattggc ctgaccgaag ccggtagaat ttactaccag
     4261 ggctgccgtc ggatgctcca tgaagtgcag gatgttcatg agcaactgta tgccttcaat
     4321 aataccccta tcgggacgct acgcattggc tgttcttcaa ctatggcaca aaatgttctc
     4381 gccgggctga cagcaaaaat gctgaaagaa tacccaggtt tgagcgtcaa tctggttacc
     4441 ggaattcctg cccccgacct gattgccgac ggtctggatg tggtgatccg cgtcggcgcg
     4501 ttgcaagatt ccagcctgtt ttcccgccgt ctgggcgcga tgccaatggt ggtgtgcgcc
     4561 gcaaaaagct atctcacaca atacggcata ccagaaaaac ccgccgattt aagtagtcac
     4621 tcatggcttg aatacagcgt gcgacccgac aatgaatttg aactgatcgc accggaaggg
     4681 atctcgactc gcctgatccc gcaagggaga tttgtgacta atgatccgat gacgttggtg
     4741 cgctggctga ctgcaggcgc tgggatcgcc tacgtgccgt tgatgtgggt gatcaacgag
     4801 atcaatcgcg gggaactgga gatcctgcta ccacgttacc agtcagatcc acgcccggtt
     4861 tatgcgttat ataccgaaaa agataagctg ccgctgaagg tacaagtcgt gatcaactcg
     4921 ctaacggatt attttgttga ggtcggtaaa ttgtttcagg agatgcacgg gcgcgggaaa
     4981 gagaagtaat taatattgta ctgttacgtt gtacaaacct gtgccaacgg ggtcccctca
     5041 ccctaacccc agaggggcga ggggaccgtt cgtacacgga gaaggattaa ttatccttct
     5101 gaaaatactg aaattattac gcggtaccgc caactgtcag gttatcaact ttcagcgttg
     5161 gctggcccac gccaaccggc aaactttgcc cttctttacc gcagacaccc acgccgttat
     5221 ccagtttcag gtcgttgcca accatcgaaa tctgctgcat ggtttcgata ccggaaccaa
     5281 tcaacgttgc gcctttcacc ggcttagtta ctttaccgtt ttcaataaga tacgcttctg
     5341 aggtggagaa aacgaatttg ccggaggtga tatccacctg gccgccacca aagttcggtg
     5401 catagatacc gtactcaacg gattcaataa tttcctgcgg ggtcgattta cccggcagca
     5461 tataggtgtt ggtcatacgc ggcatgggca gatgagcgta ggattcacgg cgaccgttgc
     5521 cagtcggcgt catccccatc aaacgcgcgt tgagtttatc ctgcatgtag cctttcagaa
     5581 tgccgttctc aatcagcacg ttgtactggc ctggcgtacc ttcgtcatca atcgccaccg
     5641 aaccacggcg atcgactatc gtgccatcat caaccacggt acacagttct gaagccacca
     5701 actccccgac ctgtccacta aatactgaag tgccacggcg gttgaagtcg ccttccagac
     5761 cgtgacccac cgcttcatgc aacagcacgc ccggccaacc tgcgccaagt actaccggca
     5821 tggtgcccgc tggtgcagca acggcagaaa gattgaccag cgccatacgc actgcttctt
     5881 ttgcccacgc atccgcacgg acttcgccgt cgagatcagc aaggaagaac tcataaccaa
     5941 aacgaccgcc gccaccactg gcaccgcgtt cgcgtttgcc atcttcttcg accaaaacgc
     6001 tcacggaaag acgcaccagc ggacgaacat ccgccgctag tgtgccgtcg gtggccgcaa
     6061 ccagaattaa ttcatagaca ccactgaggc tggcagtcac ttcctgtacg cgcttgtccg
     6121 cttcgcgggc aaccttatcg acgcgacgca gaatatccaa cttctcttca cggctcatgc
     6181 tttgcagcgg atctaccgag gtatataacg ggctatgctc taccgcgccc agcgtctgta
     6241 ctttgccatc accactatca cggacgatgg tgcgcgccgc ttgtgcactc tgttccagcg
     6301 ccagcaggct gatttggtca gcataagcaa atccggtttt ttcaccactg attgcacgca
     6361 cgccaacgcc ctgatcgatg ttgtaagaac catctttaat aatacggtct tctaaaaccc
     6421 aggattcgtg atagctcgac tgaaaataga gatcgccata atcaaggcga cgttcggcca
     6481 gttgaccgag gatcgcgaac aagtcctgat gtttcaggcc gttcgccgct agcaattgtt
     6541 cacttaccag gttcagactc atcgtttttg ctactcgtta gttactgcag tagaggattt
     6601 tttacggctg ccggatgcgg cgtgaacgcc ctgtccggcc tacggttatg ttctggctcg
     6661 ttggcctttg gcaacgatta tcatatgaga ttggggcaat tacgcgccct cgtcaaatca
     6721 ttgcgctttt tctttgcgcg gttgtcgcaa cacttcgttg atttgcggat cgtccagcgg
     6781 acccgaaatg tgatagcgca aaatggagac tttgctccac agcggtccca gcactttact
     6841 ggcggcaaac actgccgcgc caacaatggg gttaaccgca aaggccgcag ccacgcccac
     6901 cgtcgcagaa atctctggtg cgacaaccgc ttccatattc aggtcgcgac gtaccagatt
     6961 taccgaccct ttcatagcga tgtccgcttc caggccatcc accagcgtgt cgtcggtgtg
     7021 cataacgccg tctttaatcc acgcggtgct gcgaatggag tcaaaataga acccttcgcc
     7081 aaaagtgtct ctgaaatcaa aacgcagctt acgcatcagg gcatctacgc tcaataagcg
     7141 cagcaattgc ccggcatgtc cggtattgat ttcggtaatt tcgcctttac ccagttgagt
     7201 atgaatgatg ccattcaacg tcgcctcatc tggctgccag ggtgctttgc gccagtgtaa
     7261 atcgtaatcc acattaaatg acgactggcg tatgggcgtc gtgacaccaa aaaattctgc
     7321 ggcggcatca attttctgcc cgcgcagttt tcctttcagc gaggttcgtt cattccccgg
     7381 attgttaacc cattcaccct cggcagtgag ccgcgcaaaa ccagtatcaa tcagtccatt
     7441 agtcagcgtt aacgtatcgc caaaaatggt gatatcactg tcaatgtgac cgaatttttg
     7501 cccccagaac cagcactctg cgcatcgtat ttgtgcgtct ggccagccac ggaagttaat
     7561 gcgttccgtt gtcgggaaag gtgatgactg tgttgaatca ccacgagttt tcgccacgct
     7621 tgggttgtaa taaagatatt tgatattcgc cagccacggt gcgttattac gcatcgccag
     7681 cgtggcgttg atttcacgcc cttgcgcctc aaccagggtg ccatttgccg tcggttgcga
     7741 aacaatactc aggttattcc attgctgatt tcccagcgac aacataggcg tacgtaacgt
     7801 tatgtgttgt gggaaactcg ctgcaccacc gacactctcc gccgcgcctt tctggaacag
     7861 ggccggccac tcggcaccat tcatcggcgg catattgagt tcaacgccac tttgttccgg
     7921 caacggcggg agcgttttac tgtctgccgc ccaaatagca cgatcgagcg tcagcttttg
     7981 accgagcaac cagcggctat tgaaatggtt atccgcacca gcctgtccgg ttaattcaaa
     8041 gctgttgaga ttgccatcaa ccttaacgtt taccgctagt ggttcacccg caggtttggc
     8101 taacggtgaa ggtaagtgac tgctcacatt cttcaaatcg ccgttcagct ctacgttata
     8161 ggtcgcgcca gcatgataag gcagatcaat gcccacttta ccatcccacg ccacgctgcc
     8221 actcaatgct tcgttcaccg ctacaggcag aacgccggtt ttcgccggtt gccagttacc
     8281 gttgaggttt accgctacct ggtaggcttt tgccccttcc ttggtggaaa aatccacgtt
     8341 caacggctga ttaaaccagc ttgctgtcag tggttcactt tgcagatcgc cattgataaa
     8401 gctgaattta ccgctcaaat ttttcagggt gctgtcgagt ggtttgataa acagactgtt
     8461 attacgcagc gtcacttcac ctttcgcggt taccaactcg ccgttcagcg ggatatcaag
     8521 atgtaagcga gcattcacat cgccgtcgag ctggagttct tgcagggtcg cacccaggga
     8581 atctttcagc ggtgtctcat caaagtaagg gccaacggct ttacccggac ctttaatgtc
     8641 agcgtcaatc agcagttttt cttttgagta gtcagggatc actgcggtga gattactcgc
     8701 gcgcacgccg cccagattaa cgccatcggt tttcatccat aaaccgtcgt taataaagtc
     8761 cagttcaata tcaaggttag ttaatgcagg ccagtccggc tggaaggcaa acttcgcgtt
     8821 gcgcagcggc accagcactt caaactgacc ttcgttgtgt ttatagggga agagttgcgg
     8881 attgccacca taaaccagcg tcgcgttatc cgcttcaccg ccctgaatcg cgccacttaa
     8941 gtaatcaacc aggtctttac ccatcaagtt ttccgggaaa tagcgccagg cttgtgaacc
     9001 atcatcggta ctgatgccag ccagaatacc cagccagggt tcatcgttag caggttgcag
     9061 gtaacgaaaa ccgccgcgcg catggacggc tttggcttta acgtcaatat tacgcccatc
     9121 cagctggaaa cctttgtcat tgttcagcca gcttatcgtt gcttggccgt cggcgatttc
     9181 cagtggcgca cggaataccg tttcgtaagg catctttgcc tgcttcatcg acgcagtaag
     9241 caaaccattt tctacgctgc cggaaagcgt cccggagaag tgttccgcac ccggtaataa
     9301 tttccattgc ttccaggcca gatcgctcca cgatgcctga aaacgggtct tgtctgccgc
     9361 ctgaagcggg atatccagcg ccagagtgtt aatcttgccg ctcggttgtg tggagcgcca
     9421 aacatcaccc agtgcaggtg aaagtttcgc gaccagcggg cgtacgccct ccaggcctgc
     9481 cagctccaga ttactggcgc gaatccggag ttcgtcactg cgtttattgt ctttgccgcc
     9541 aacgtcctgt tccggtatcc aggccagcgt caatgctccg ctcggccagg gtttgccgtc
     9601 catcgtgatc cgtgtatcgg gaatagagaa ctgccatccc ggattttcac gcgtaatatg
     9661 cgcagtcaga ttatccaccg acagcgtatg tgtttccttc tcgcccaacc agctggcacc
     9721 gccctgtttc agccagacgt caccgccggt tacatcgcct ttgtcgatcg tcatccagcc
     9781 ttcaagggag aactgtgccg tttccagcgc aatattgtcc tgcatccatt taccgagcca
     9841 cggcttcagg tcgatgtcat ccgcctggag ccagacgcga ccattgctta acaacccctc
     9901 atcatcgcgc aaatccatac gcacctgcat cacgccgtgc tgtccggtaa ggctggagag
     9961 gcttaccagg ccttccgcac ggtgtcgacg tggatcgttc agccaggtga gctgtgggat
    10021 cgccagctcg gcgcgctgac cggatggcgt caggaaactg acttcactgt cgcggagatc
    10081 gaaatggtca aattgacgaa gaaacagatc gctgatgtga ctggcttcca ggctatcatt
    10141 accaccgccg ctggtgatag gagtgttggt gcgaaagcgc agctgccaga aagtgaggtc
    10201 gcgaaactgc cagcgcatat gtaacaggct ctgccagaca tccagcgcaa gagtaacgcg
    10261 tttaaccgaa aattcgccgc catcttttag ttctgcacgg atgtcgtgcg cttcaagcgt
    10321 cggaccaaaa ttctgccagc tggctgagag ctgactggct tctaccggca tgccagtcgc
    10381 ggattctatt ttgttgagga tttccggacg ccaggcgtca agatgcggta aagcaatacg
    10441 caggccgcta accagcaggg cagcgatcac aacgagcgcg gctccagtaa gcagtaaaat
    10501 ccccggcaat cgcctcaccc gtcactcctt gtctgctaaa aatgtgactc aaaaaccctt
    10561 tgccggatgg cggcccagca tctgtttaca tcattacgac gtcaaactgc tcctggttat
    10621 agagcggttc aatttgtact ttaacctgtt tgccaacgaa aatttccact tccgccagcg
    10681 agtgtgactc ttcgcctttc aaggcttcgg ctactgccgg agaagcatag accaggaaac
    10741 ggtcggagtc gtaagcatgg tggacacgaa caatctcgcg catgatttca tagcataccg
    10801 tttccacggt tttcaccgtt ccgcgaccgt ggcaggttgg gcattcgtta cacagtacgt
    10861 gctcaatgct ttcgcgggtg cgtttacgcg tcatctccac cagccccagc gccgaaaaac
    10921 cattaacgct ggttttcacc cgatctttgc tcagcgcctg ctccagcgag tgcagcacgc
    10981 ggcgacggtg atcttcatta ttcatatcga tgaaatcaat aatgataatc ccgcccagat
    11041 tgcgcaaccg taactggcga gcgatagcct gcgtcgcttc aatattggta ttgaaaatgg
    11101 tgtcgtccag attgcgatga ccgacaaacg ctccggtatt gatgtccacg gtggtcatcg
    11161 cttcggtctg gtcgataatc agataaccac cggatttcag ttctactttg cgttccagcg
    11221 ctcgctgaat ttcgttttcg acatcaaaga gatcgaaaat cggctggcgt ccggtgtaat
    11281 gctccagctt gctggtcatc tcgggaatgt actccgaggt aaactccagc aatgcttcgt
    11341 aagtcaggcg tgagtcaacg cgaatgcggt cgagttcggc gtcggcgaaa tcacgcagaa
    11401 cacgctgcgc cagcgccagt tctccgtaca gctgataacg ggtctgcggg cgttttttac
    11461 gctccataac tttggtccag acgcgtttca gataagcggc atcggaggcc agttcagcct
    11521 cgccaacccc ttccgctgca gtacggatga taaacccgcc ctgctcgtcg caatactctg
    11581 cgaccacttt tttcaggcgt tcacgttctg attcgctttc aattcgttgg gataccccaa
    11641 cgtgagaagc ccctggcata aacaccagat agcgagaagg cagcgtgata tcggtggtca
    11701 ggcgcgcacc tttagtgcca agcggatctt tcaccacctg caccatcaga tcttgcccct
    11761 gacggaccag ttccgagatg tcgcgcaccg tgaattgctt ttgttcttca cccgccacac
    11821 attcagtgtg cggcatgatg tcagatgcat gaagaaacgc ggctttatcc agcccaatat
    11881 ctacaaaagc cgcctgcata cccggaagta cacgacttac acgacccttg tagatattgc
    11941 ctactattcc gcgtcgcgcc tcacgttcaa tatgaatttc ctgcagaata ccgccatcaa
    12001 tatacgccac tcgagtttcc gaaggcgtta cgtttaccaa caattcagcc gtcatgttta
    12061 tcccttttct cacgcagtgc gttaaaatta cttaataatt catacgtttc aaccagcggt
    12121 aagccgacta cggcgtgaaa gctgccattt atcttcctga caaaacagcc acccagcccc
    12181 tgaataccgt atgcacctgc tttatctaac ggttcgccgc tggcgacata gcccgcgatg
    12241 tcttcgtctg ttaacgttct gaaagtcaca tcggtgacca ccaggcaatc gagaatgtgc
    12301 tggcggtcag ccaacgccac tgccgtcatc acctgatggg tctgacccga taatttgcgc
    12361 aacatctgcg ccgcatgctc tgcgtcgcgc ggtttctcga gcacttctcc gttcaggata
    12421 acgatagtat ccgcacccag caccgggaga tcctgcgccg tttgcgcgac acccgcctgt
    12481 gctttctcgc gcgccagacg cacaacatac tgctgcgcgc tctcctgcgg ctgacgctgc
    12541 tcctcaatgc ccgtaacaat acgttcaaag gtcacgccaa gttgcgcaag taactcctga
    12601 cgacgcggag aaccggaagc taaatacaga gaagtcatag aaacctttat tgcactgcaa
    12661 actgctgacg gactttacgc atcagcaaga aaatccacgg ccagagcacc ccattgacta
    12721 cactactcca gaacacttcc ggtctgaaag agacgttaat cactaaaaac tctgcccaga
    12781 aaacaataat atccaccacc agcgaaagca acatgacgac cagcgcctgc tgccataatg
    12841 cgaggttgcg gaaaagctgg tatttcagcg ccaccaggta agcaatgatg ctcatcgcca
    12901 atacgcgtac gccaagcgtc gagccgctga tcagatccag tatggcaccc atcacaaaac
    12961 ctgtgcccac atttacgcga tggggcaagg ccaggatcca atacaacaag atgagtaaca
    13021 cccagtttgg ccggaaaaca atcaggttat ccggccaggg catgatttgc agcaacagcg
    13081 caatgaggaa agagagccag attacccagc gtccctggct acgatagctc gccactattg
    13141 tcctcccggc gcacgcgcag gcggttgagc accactttgc ggcggcgtag ccctttgtgg
    13201 agagcgatta gcagcgggct gtgtgggcgc agcaggcgca gtaactgcat ttcctgtcgc
    13261 cggttgctgc ggagtcggct gagtgatccc cgttgccggt tcaggtaact ttggccccat
    13321 cgcgtctggc gaaggcaata cctgcggcat catctgcatc aaacgttcat tagcaacacg
    13381 atgcacctct tccggcgtca tcgggttagc gccgttacga tccgcgcccc acagcagcag
    13441 cagataacgc aaacgttgca gtcctgcagt cggacgcgcc tgaatcacag tataagcgcg
    13501 ctgggtatcg agttttacgg aagagacaac cgcgaccgga tagccttccg ggaaacgacc
    13561 gcccagaccg gaagtcacca gcacatcacc aacacgaata tccgtattcg ctggcagatg
    13621 ctcaagctgc aaatcatccg tacaaccgtt accggctgca attacgcgga tatcgttgcg
    13681 cagcacctgg attggcagcg cgtgggtcgc atcacaaatc agcagcacgc ggctggtcag
    13741 tttagcgacg gccaccacct gaccaacaac acctttgtcg ctgataaccg gctggccttc
    13801 ataaacgcca ttaacgctac ctttatcgat aacaacttga tcgctataag gatcgttaac
    13861 cgtggagata acctgagtca ccattttctg ctcatcctga cgcagcgggg aacccagtag
    13921 ctcgcgcaga cgcgcgttct cctgtttgta ttgtccaagc atcagcagtt cactgttttt
    13981 cagcaacagt tcctgacgta acgcccggtt ttcaagttct agttggtcac gcgaggccag
    14041 cgtctgcgat acgccatcca gcaattcacg aggagcattg gaaacaaagt agaaaggact
    14101 gacggcggta tccatataag tacggatttg actgaacgtc cccaggcggc tgtcggcaat
    14161 aataatgccg agcgccacca gcaccgccag aataaggcga atctgtagcg acgggccacg
    14221 gctaaaaatt ggcttcataa gttatgcgta ttctcgtatc agaccaggca gggtaaacag
    14281 acacttcccc tgcctgcatc cgattactct tcgctgaaca ggtcgccgcc gtgcatgtcg
    14341 atcatttcca gcgctttgcc gccaccgcgc gccacacagg tcagcgggtc ttcagcaaca
    14401 acgactggaa tgccggtttc ttccattaac aaacggtcaa ggttacgcag cagtgcgcca
    14461 ccaccggtga gcaccatgcc gcgctcggag atatcggaag ccagttccgg cgggcactgt
    14521 tccagtgcaa ccattaccgc gctcacaata ccggtcagcg gttcctgcag tgcttcgagg
    14581 atttcgttgg agttcagggt aaaaccacgt ggaacacctt ctgcaaggtt acggccacga
    14641 acttcgattt cacggacttc atcgcccggg taagccgaac cgatttcgtg cttgatacgt
    14701 tctgcggtgg cttcaccgat cagagaaccg taattacgac gcacatagtt gatgatagct
    14761 tcgtcaaaac ggtcaccacc aatgcgcacg gaagaggagt aaaccacgcc gttcaaggag
    14821 ataacagcaa cttcagtggt accaccaccg atatcaacca ccatagaacc ggtcgcttca
    14881 gaaaccggga ggccagcacc aattgcggca gccatcggtt cttcaatcag gaagacttca
    14941 cgggcaccag cgccctgcgc ggattcacga attgcgcggc gttcaacctg ggtcgcgcca
    15001 accggcacac aaaccagaac gcgcgggctt ggacgcatga agctgttgct gtgcacttgt
    15061 ttgatgaagt gctggagcat tttttcagtc acgaagaagt cggcgataac gccgtctttc
    15121 attgggcgaa tggcagcaat attgcccggc gtacggccca gcatctgctt cgcgtcatga
    15181 cctactgcag ctacgctttt cggtgaaccg gcacgatcct gacgaatggc caccacggaa
    15241 ggctcattca atacgatgcc ttgtcctttt acataaatga gggtattcgc agtacccagg
    15301 tcaatggaca agtcattgga aaacatgcca cgaaattttt tcaacatact aagggataat
    15361 cctgaaagct ggggcggaaa agaaaatccg cttactttac caaccacacg cagcagcgac
    15421 aaggcgcaaa aatcatctgc tacggtgaaa attagtgcag ttcgtttcct ttgttacaaa
    15481 tctctgcctg agtccagaaa ggcttaatgc atcagcagca ttcctcgcct gtttgcaacc
    15541 gcgtaaggtc attcatctgc atatgtgctg caacaatctg gcgagcagac aagcacactc
    15601 ccatgagacg cagcgcgcgt tattctacgt gaaaacggat taaacggcag gttaaaccga
    15661 gtatctttgt gaatattttt tcacgttagt atcaagtggc tgtgaggacg cgaaaaaatc
    15721 cccttgccca cctgtaacac cgcgctgaat cagggtctgc cactcgcttc gcgaacgtac
    15781 gccggtggcg taaacctggg tgctggtccc ggagcaggct tccaccaggc tttgaaccag
    15841 cagctggttc tccgttcgct tctcaatgtt tctgaccagc cccggatgga gcttgagtaa
    15901 ctcaacatta agttctttga tccaactggt gcttaccagc gtcaaaccag cctggttgac
    15961 ggctacccgt acccctaaag cattcactaa acgaataaca ggttgtaagc gactgatatg
    16021 ttgaccaaca tcggcctctg caagttcaat aattatgcgt cttcgttgcg atttttcaca
    16081 ttgcattaac gtatcgcgca gccaacgctg aaaacgcggg cgaatcagcg actcaacggt
    16141 aacctgaatc gccagatttt cctctggcca gtaacgcaat agtggaataa gacggctgat
    16201 tagcaggcgg tcatactctt ccgataaacc aaactgcaag accatcggca tatactctgc
    16261 cgagctaacc tcttcattac catcgaagat gcggcacatg agttcacgat gatgaacccg
    16321 gccttcgcga gtaaccgccg gtttttgata aaggcgcggg ccgccgcgac tgagcatttg
    16381 ctcgataagc gtacgccagc gaacattacc gcgtcctttt tcaggcaacg agtcatcgta
    16441 aatagcccag ctattgccac cctgcaatcc cgcattacgc gtggcagact ctgcatgttc
    16501 cattacctgc tcggtatcct gaccactacg ccaggcacag ataccaatgt ggagcatatc
    16561 gtcgcgatcg agcattttat tgttcggcaa ggtatcgacg gctttgatta actgactggc
    16621 gatgctctct gcctctttta acgtccggtg cggtaacagc gcagcaaaat cactgcggtg
    16681 gtaacgcgcc agcagtgcgc cagggtagcg catcataaat gtcgatagca gattcgtcag
    16741 agagaagaac tgttcttcaa cctggctgtg cccccaggta tcactcaaca tattgaaatc
    16801 cggcagacga atcatcatca cgatcccgtg ggtacctact ttctcttgat cttccagtaa
    16861 cgttgctaac tgattatcga aaaagagtcg gttattgagg ccggttttca tgtcctgggc
    16921 ggcataagag cggatcagcg tatcaagacg gctgtgttgt tcgcgtgcgt tctgaatttc
    16981 acaaagcagc gtatccagcg cactgctggt tctgggcggc cattcataga tggttcccaa
    17041 cacattagag ccacgctcac cgtttaagat acgagtagcc cgggtttcca gcaattcttg
    17101 cccggcaagt tgccgttgta accagcgtac cgccaggaag agcataagaa tgataaagcc
    17161 aatcgccccc gtgagcggcg cggtagtcat taaagaatgg aaatagttgc ccatcggatc
    17221 ctgataaacc agacgcagcg acatccccgg atgctttatc aacggaacgc tcagttcacg
    17281 aaacagatcg ttggtgccaa ccggacgata actaccattt ctggccaggg tataaacctg
    17341 cttatcacca tggagcaggt ctacacgaac gatatctgcc gacatcatta attcggtaat
    17401 ttgtggcctt aatgtgctga agtcattcga cactaggtgg gtatcaatcg ccgtcgccac
    17461 cgcctgaaca cgatgactaa acttatactg aatggcgttg tagaaactta gcgaacagcc
    17521 cagcaaagtc acaaaaattg ttaacccggt gagcagcgtg acaaaggccg aaaatttcgt
    17581 cgttaatctc atccttgtgt taactccgat agtgaggaag cgggcatact agcaaatcag
    17641 atttatctcg caatttattg cgcttcatcg gctttgcttt tccattagcg agtatagtct
    17701 tcagaaatta ttttccaatc catcatgcac atgaggacca cttatgcagg cgttactttt
    17761 agaacagcag gacgacaaaa ctctcgcatc agtacagact ctggacgaaa gtcgcctgcc
    17821 ggaaggcgat gtcacggttg atgttcactg gtcgagcctg aactacaaag atgcgctggc
    17881 gattaccggt aagggaaaaa tcatccgtaa ttttccgatg attcctggga tcgattttgc
    17941 cggaactgta cgcaccagcg aagatccgcg ttttcatgcc ggtcaggagg tgttactcac
    18001 tggctggggc gttggtgaaa accactgggg tgggctggcg gaacaggcgc gagtgaaagg
    18061 tgactggctg gttgccatgc cgcaagggct ggacgcgcgt aaagcaatga ttatcggtac
    18121 tgccggtttt accgccatgc tgtgtgtgat ggcgctggaa gatgccggtg ttcgcccgca
    18181 ggatggggag attgtcgtga cgggtgccag tggtggcgtc ggcagtaccg ccgtggcgct
    18241 gctgcataag ttgggttatc aggtcgttgc tgtttccggt cgcgaaagta cccatgaata
    18301 tctgaaaagt ttaggtgcca gccgtattct tcctcgcgat gagtttgctg aatcccgtcc
    18361 tctggaaaaa caagtctggg ctggggcaat tgacaccgtt ggcgacaaag tgctggcaaa
    18421 agtgctggcg caaatgaatt acggcggctg cgtggcggcc tgtggtctgg cgggtggttt
    18481 tactctgcca accacggtca tgccatttat tctgcgtaat gtccgtttgc aaggggtgga
    18541 ttcagtaatg acgccaccag cacgccgcgc acaagcctgg cagcgactgg tcgccgattt
    18601 accggaatca ttctataccc aggcggcaaa agagatatct ctggcagagg caccgaagtt
    18661 tgccgaggcc atcattaata accagatcca gggtcgcacg ctggtgaagg ttaactaacc
    18721 atttagcaag gattaataag agagggaact cattttgaaa tcattattca atcggttaac
    18781 ggaaaaagcg gttagtcgga cagctttcgt cgaacacctt ggtcaggaag ttgtacaaca
    18841 tcatccaaac tggaaagtca tgatttcgac tgaccataaa ttgatgcgca ttgatacggg
    18901 gcaccaaaca gccattattg atacgcctcc gtcgcctgtt aggtttatgg tgctttgcct
    18961 tggcgacgct acgcttaatc tgttactgat ttctggtacc atggggggaa taatctgatt
    19021 ttatttgatt acaaattaac cacttaaacc tattgaatgc ggaacattgt gagtcggtta
    19081 attttgcgtg ctttagcatt cacatctttc cggacgatgc agtgaaaatt gggtaatccc
    19141 cagcaaccgc tgcgtaatgt cgtctatctt gtcgcgatcc tggcatccct acattatttg
    19201 tggtctgtga agattatctc attgcagccc cccatcttcg cagggctggc tttacagctt
    19261 ttcaccttac gttataagaa gttccgtcga tgatggcgct aatttcgtga attgtgcggc
    19321 ttgttgcaaa ttacacggtg ttgaaggtta tttacatgtt agctgttgat tatcttccct
    19381 gataagacca gtatttagct gccaattgct acgaaatcgt tataatgtgc gacctcgtcc
    19441 tccctgacgc agtttttgcg ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt
    19501 gttttttgcc ggaggatagc agcagatcgc tgcacaatgt ccgtcaagtc taacattgac
    19561 actctggggc aaaatagacc ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc
    19621 tgtcggggca tacgctttac agacggcggt gaaacgcctg tcacaatcac actaaacaaa
    19681 gagtacggaa cccactcatg gatattcgta agattaaaaa actgatcgag ctggttgaag
    19741 aatcaggcat ctccgaactg gaaatttctg aaggcgaaga gtcagtacgc attagccgtg
    19801 cagctcctgc cgcaagtttc cctgtgatgc aacaagctta cgctgcacct atgatgcagc
    19861 aaccagctca atctaacgca gccgctccgg cgaccgttcc ttccatggaa gcgccagcag
    19921 cagcggaaat cagtggtcac atcgtacgtt ccccgatggt tggtactttc taccgcaccc
    19981 caagcccgga cgcaaaagcg ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc
    20041 tgtgcatcgt tgaagccatg aaaatgatga accagatcga agcggacaaa tccggtaccg
    20101 tgaaagcaat tctggtcgaa agtggacaac cggtagaatt tgacgagccg ctggtcgtca
    20161 tcgagtaacg aggcgaacat gctggataaa attgttattg ccaaccgcgg cgagattgca
    20221 ttgcgtattc ttcgtgcctg taaagaactg ggcatcaaga ctgtcgctgt gcactccagt
    20281 gcggatcgcg atctaaaaca cgtattactg gcagatgaaa cggtctgtat cggccctgct
    20341 ccgtcagtaa aaagttatct gaacatcccg gcaatcatca gcgccgctga aatcaccggc
    20401 gcagtagcaa tccatccggg ttacggcttc ctctccgaga acgccaattt tgccgagcag
    20461 gttgaacgct ccggctttat cttcatcggc ccgaaagcag aaaccattcg cctgatgggc
    20521 gacaaagtat ccgcaatcgc ggcgatgaaa aaagcaggcg ttccttgcgt accgggttct
    20581 gacggcccgc tgggcgacga tatggataaa aaccgcgcca ttgctaaacg cattggttat
    20641 ccggtgatta tcaaagcctc cggcggcggc ggtggtcgcg gtatgcgcgt agtgcgcagc
    20701 gacgctgaac tggcacaatc catctccatg acccgtgcgg aagcgaaagc agctttcagc
    20761 aacgatatgg tttacatgga gaaatacctg gaaaatcctc gccacgtcga gattcaggta
    20821 ctggctgacg gtcagggcaa cgctatctat ctggcggaac gtgactgctc catgcagcgc
    20881 cgccaccaga aagtggtcga agaagcaccg gctccgggca ttaccccgga actgcgtcgc
    20941 tacatcggcg aacgttgcgc taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact
    21001 ttcgagttcc tgttcgaaaa cggcgagttc tatttcatcg aaatgaacac ccgtattcag
    21061 gtagaacacc cggttactga aatgatcacc ggcgttgacc tgatcaaaga acaactgcgt
    21121 atcgctgccg gtcaaccgct gtcgatcaag caagaagaag ttcacgttcg cggccatgcg
    21181 gtggaatgcc gtatcaacgc cgaagatccg aacaccttcc tgccaagtcc gggcaaaatc
    21241 acccgtttcc acgcacctgg tggttttggc gtacgttggg agtctcatat ctacgcgggc
    21301 tacaccgtac cgccgtacta tgactcaatg atcggtaagc tgatttgcta cggtgaaaac
    21361 cgtgacgtgg cgattgctcg catgaagaat gcgctgcagg agctgatcat cgacggtatc
    21421 aaaaccaacg ttgatctgca gatccgcatc atgaatgacg aaaacttcca gcatggtggc
    21481 actaatatcc actatctgga gaaaaaactc ggtcttcagg aaaaataatt tgtgacgcgc
    21541 ggtgtttcca tcgcgcttag caaagaacgt aatcaacgcc gggcctgtta atgctacccg
    21601 gcgtttttat tgccgttaac taacggtagc ctgcaatttc cgctttcaac gcgctaattt
    21661 cagcatcgcc atcaacaatt cccagctcaa agcagtattt ctttgtttcc cctggctcaa
    21721 taaagggcag acgcccctgc gctcgttctt tatcacgacc gtcaggaaaa ctattactga
    21781 cctccagccc cataacatat tcacccgcgc ctaaattttt ccactggata aagttgggta
    21841 gctgcatggt atcgaatttc tcgtaaattc cccaccccag ttcggcgttc accagcgcaa
    21901 ctttactcat cccctgactg tcgctattca acgtgagata gtaaacctgc tcgtcaaaac
    21961 cgggctgtgg ggtacaaatc tccagatgac tggctaaccc ttccgccgca tgcagagttc
    22021 gtggcgttac gcttttagcc gacagcaaaa tttctgcctg tggcgataat aaaggccagc
    22081 cgtaattaaa gtgataaaga atttgcagcg gctggcgggt gaaaccatga ttagtcacct
    22141 tgtcttcaat gcgcattaca ttttcgccat aacggcagcg aatggtcctt tccagcgtca
    22201 gattttcacc gaacaatcgt gtttcccgta ctttgccatt gatgacaaat tcaatgctgt
    22261 cgtcggtccg ttctgtgcga taccccacct cttctgctgg agtcgcagcc aaacgcccgt
    22321 gtaaccccag cgtttctcct tcatcctcac agggcgtgcc aatgtagcta agtccgcagg
    22381 tagtcagtaa ccccgcgtaa aagctacgta aaaacccgtt gccctgagac tcaaaaaaag
    22441 cgggggcgac aacaccgttg ggggtgataa aagagagcgc agtgtccttg tagctggccc
    22501 atgcaatatc cagcgcgcga tcttttacca ccgtaaatgc cagtcccgca ccagtacgga
    22561 catcaaacgc ttctgtaccc tttgctctgc cactacgtaa ctcatagtgt ttaattccgg
    22621 caacctgcga gagatcgcca atccacgacc aaatttcgtt tttcattgtg ctgcttcccg
    22681 aatggacatg tcatggcgct ggatgaacgt ttcgatttcc gcaaggcgag acgttccctc
    22741 cattgggcca cgtcgggtta ccgccagtgc accgcaggcg ttggcatatt gtaatgcccg
    22801 atgggcgtca aaccctagct ggcggcaggc aatccatgcg ccaccaaaac agtctcccgc
    22861 accggtcggg tcaacttctt ccaccggata actctcaaca tgaaactgct cattcgcaga
    22921 gtagtaactg gctccttgat tgccgcgctt cacaatcacc tctttaacac cttcttcaag
    22981 aaaaccagcg attgcacgct ccggcgtcga gtgtggcgac aagagcaaca cttctccctc
    23041 actgggcata tagatatccg ttaactcaag aacaaagtgg agggcgtcac gcatttctgg
    23101 aatatcgagc atctctttcc gaatattagg gtcaaaagaa ataacgccac cattctcttt
    23161 tacaatggtt accgctttct ttactgcgtc taccatatgg aatgaaaaca gggatgaacc
    23221 cataatatga aaatgggtac agtccttcag aatattttca tcaacatgct gcgccgataa
    23281 tttaccgcaa gcggcattct tgatattaaa aataaaatca cgatcgccag agttatgata
    23341 agtaacaaag gcactcccgg tagcttccag aggtaagact gaaatccccc tgatatccac
    23401 accgtcagca gccagacgat gtatattaat atcaccaaag ccatcattcc ccacacaact
    23461 aatgatgccg cacggaacgc caagacgtgt cacctggtcg ataaaaatag cgggcgcacc
    23521 actgggataa gggccgttcc agatacctgg ttgatcaaat ggctggccga ttttactcgc
    23581 cataatttca accagaattt ctccaattgt gaagacctta ctcatatgat taattccttg
    23641 aaatgattag ctgcgttttc ttttatttct tacgtcaaaa taaacggcta caagaatgac
    23701 taatccttta accaccatct ggtaaaaata aggaacattc attaagttca tgccattatt
    23761 aataacgccg atggttaatg cgccgatcac cgtccccacc acggttccat agccgccgtt
    23821 caggctggtg cctcccagta cagctgcggc aatagcatcc agttcgtagc tcaacgcgga
    23881 attcggttgc ccggaataga ggcgtgaggc aaggatcatc ccgttgatac ctgccagcac
    23941 ggctgtaatc ataaagactt taattttcaa gcgggtgaca tttatccctg aataaattgc
    24001 tgcttcttta ttaccaccag tgatataaat ttttcgtcca aaggttgttt tagtaagaat
    24061 gaagtggttt atcagcaata agatgattaa tatccatata ggaataggta tatagagaaa
    24121 ttctccattc cccagcgcca gaaaagcatc atctttaatc atgatgggca taccatcggt
    24181 cactatataa gcgataccac ggaagatccc catagtagca acggtgacaa taaacgaagg
    24241 gatccccgct atagccgtca agccaccatt tatcatcccc agagccagtg ctgcaataat
    24301 ggataatggg accaataaaa caaatggaat tccccatgaa gccccaagcg cagcaaatgt
    24361 gccaatcaat gcaatggttg aaccgaccga taaatcaata tcgccaagca aaagcacata
    24421 agtcattcca taggcggtaa ttgcaattat cgaaacctga agaataatat tagtaatgtt
    24481 attggcggta aaaaaactgt cattaataaa actgaacaga atgattaaca ccaccattcc
    24541 actgaggata ccaccatatt gcctgaagac ttttttaaaa ttatttgcaa tgggaatttc
    24601 cgaaggtttc ggactggcaa gagttttctc tgtcatttaa cgtactcctg ttgcagcggt
    24661 cattacctgt gtttgcgtta aattttcaca tgaataaata tcggcaatcg ttttattacg
    24721 cattaccacc agacgatcgc taagtgccat gacttctggc agttcagagg acaccagaat
    24781 aattgctgtc cctgccgcag ccagttgacg tatcacgtta tagatttcaa attttgcgcc
    24841 gacatccact ccgcgcgttg gctcatcgag caataaaata cgcggagttt tctccagcca
    24901 cttcgataac acgattttct gttgattacc gccgctcagt gctcccactg cctgatgcac
    24961 agaactcact ttagtgttca ttctggcaat tgattgcagc gtcttcgcct cttcttgtcg
    25021 gtgattgatg agtcccatcc gcgaagcgtt atattgaaac gccacatggt gcgtgttcat
    25081 attgacgtcg tgcatcagca ctaacccttc ttccttgcgg ttttcggtca caaaagcaat
    25141 gccatgatca atagcctgtt tgggattagc gatcctgact tccttaccat caatcaacac
    25201 agtcccgtga tacgaaacga ggccaaatag cgccttcatc acatcggaac gcccggcccc
    25261 caccaggcca aaaaatccga ggacttcccc cggacgcaca gcgaaactga cctctttaaa
    25321 aagcgaagga tgggaaagat tttttacttc cagttttgca ggaacatttt catccggttt
    25381 ttccccaaga cgtgctggcc aaatattttt catttcccga ccaaccattt gggcaatcaa
    25441 gtgctggata tcactgtttt tcttttcatg cgtagcgata tactcaccgt cgcggatcac
    25501 cgaaatatca tctgaaatcc gcataatttc atccatgcga tgagagatat aaataaccgc
    25561 acagcctcgc tgctttaatt tttcaataat gttaaacaga atctccgctt cattatcact
    25621 tagcgaggat gtcggttcat ccataatcac gacatccgct gcatagctta aggctttggc
    25681 aatttctacc agttgcctat gtgccatcga catattgccg accatttcca gagggtctag
    25741 tgggatgccc agttccgcga ggaggttctg gcaatctgcg agcatttttt tatcgttaat
    25801 gatgccaaaa cgtcgcggtt cattattggc ataaatattt tccgccacgg tcatattatt
    25861 actcaggctc agttcctgaa aaataatcgc aatccctaac tggcgagaat gctgcggatt
    25921 attaatggtg atggtttcac cacgtagtgt tatcgttccc tgggttggcg gataaacacc
    25981 ggccagtatt ttcattaatg tcgatttacc ggcaccattc tcgcctaata aagtatgcac
    26041 ccgcccttta cgtaaagaga gtgtaatatc ttttaatgcc tgaacatgac caaatgtctt
    26101 cgcaatatgc gaaacatcaa gaataacatc cgtcataatg acctcggtgt cacaacaaaa
    26161 cgccaataac gttttgttgt gaggtgaaga tatttactat ttacctgtat aaacgccggg
    26221 ttgtaccggg atcatttttt ctaccggttt accattgagg tgatcaacag cggcatcaat
    26281 ggctttagcc cccatttgat cgggatattg cc
//
LOCUS       DAZGJT010000067        25828 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZGJT010000067 DAZGJT010000000
VERSION     DAZGJT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.067"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(281..2854)
                     /gene="clpB"
                     /locus_tag="ACHKFK_003001"
     CDS             complement(281..2854)
                     /gene="clpB"
                     /locus_tag="ACHKFK_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO9053288.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HYRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2984..3715)
                     /gene="yfiH"
                     /locus_tag="ACHKFK_003002"
     CDS             complement(2984..3715)
                     /gene="yfiH"
                     /locus_tag="ACHKFK_003002"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO9053289.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRTGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASTAFIQHGDKYLADIYQLARQRLASVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3712..4692)
                     /gene="rluD"
                     /locus_tag="ACHKFK_003003"
     CDS             complement(3712..4692)
                     /gene="rluD"
                     /locus_tag="ACHKFK_003003"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO9053290.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEEMRADFEEHKDEVDWL"
     gene            4827..5564
                     /gene="bamD"
                     /locus_tag="ACHKFK_003004"
     CDS             4827..5564
                     /gene="bamD"
                     /locus_tag="ACHKFK_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO9053291.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5834..6175
                     /gene="raiA"
                     /locus_tag="ACHKFK_003005"
     CDS             5834..6175
                     /gene="raiA"
                     /locus_tag="ACHKFK_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO9053292.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6278..6403
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6279..6326
                     /gene="pheL"
                     /locus_tag="ACHKFK_003006"
     CDS             6279..6326
                     /gene="pheL"
                     /locus_tag="ACHKFK_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO9053293.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6425..7585
                     /gene="pheA"
                     /locus_tag="ACHKFK_003007"
     CDS             6425..7585
                     /gene="pheA"
                     /locus_tag="ACHKFK_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO9053294.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSAINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHSLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7628..8749)
                     /gene="tyrA"
                     /locus_tag="ACHKFK_003008"
     CDS             complement(7628..8749)
                     /gene="tyrA"
                     /locus_tag="ACHKFK_003008"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO9053295.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVSDAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYSQRFQSESRVLLRQANDNRQ"
     gene            complement(8760..9830)
                     /gene="aroF"
                     /locus_tag="ACHKFK_003009"
     CDS             complement(8760..9830)
                     /gene="aroF"
                     /locus_tag="ACHKFK_003009"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO9053296.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            10040..10405
                     /gene="yfiL"
                     /locus_tag="ACHKFK_003010"
     CDS             10040..10405
                     /gene="yfiL"
                     /locus_tag="ACHKFK_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO9053297.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDTFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWRKGADENASTIRLN"
     gene            10553..11071
                     /gene="yfiR"
                     /locus_tag="ACHKFK_003011"
     CDS             10553..11071
                     /gene="yfiR"
                     /locus_tag="ACHKFK_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO9053298.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPGLSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAIISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            11061..12287
                     /gene="dgcN"
                     /locus_tag="ACHKFK_003012"
                     /pseudo
     CDS             11061..12287
                     /gene="dgcN"
                     /locus_tag="ACHKFK_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            12303..12785
                     /gene="yfiB"
                     /locus_tag="ACHKFK_003013"
     CDS             12303..12785
                     /gene="yfiB"
                     /locus_tag="ACHKFK_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9053299.1"
                     /translation="MIKHLLAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     VLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12862..13209)
                     /gene="rplS"
                     /locus_tag="ACHKFK_003014"
     CDS             complement(12862..13209)
                     /gene="rplS"
                     /locus_tag="ACHKFK_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO9053300.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13251..14018)
                     /gene="trmD"
                     /locus_tag="ACHKFK_003015"
     CDS             complement(13251..14018)
                     /gene="trmD"
                     /locus_tag="ACHKFK_003015"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO9053301.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(14049..14597)
                     /gene="rimM"
                     /locus_tag="ACHKFK_003016"
     CDS             complement(14049..14597)
                     /gene="rimM"
                     /locus_tag="ACHKFK_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO9053302.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14616..14864)
                     /gene="rpsP"
                     /locus_tag="ACHKFK_003017"
     CDS             complement(14616..14864)
                     /gene="rpsP"
                     /locus_tag="ACHKFK_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO9053303.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15001..16362)
                     /gene="ffh"
                     /locus_tag="ACHKFK_003018"
     CDS             complement(15001..16362)
                     /gene="ffh"
                     /locus_tag="ACHKFK_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO9053304.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16529..17320
                     /gene="ypjD"
                     /locus_tag="ACHKFK_003019"
     CDS             16529..17320
                     /gene="ypjD"
                     /locus_tag="ACHKFK_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO9053305.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIFQQLIS"
     gene            17341..18627
                     /gene="yfjD"
                     /locus_tag="ACHKFK_003020"
     CDS             17341..18627
                     /gene="yfjD"
                     /locus_tag="ACHKFK_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO9053306.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18682..19275)
                     /gene="grpE"
                     /locus_tag="ACHKFK_003021"
     CDS             complement(18682..19275)
                     /gene="grpE"
                     /locus_tag="ACHKFK_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO9053307.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19398..20276
                     /gene="nadK"
                     /locus_tag="ACHKFK_003022"
     CDS             19398..20276
                     /gene="nadK"
                     /locus_tag="ACHKFK_003022"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO9053308.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQQADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20362..22023
                     /gene="recN"
                     /locus_tag="ACHKFK_003023"
     CDS             20362..22023
                     /gene="recN"
                     /locus_tag="ACHKFK_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO9053309.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSQLQEMT
                     ARYHLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQHYANELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22172..22513
                     /gene="bamE"
                     /locus_tag="ACHKFK_003024"
     CDS             22172..22513
                     /gene="bamE"
                     /locus_tag="ACHKFK_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO9053310.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22575..22865)
                     /gene="yfjF"
                     /locus_tag="ACHKFK_003025"
     CDS             complement(22575..22865)
                     /gene="yfjF"
                     /locus_tag="ACHKFK_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO9053311.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDIVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22855..23292)
                     /gene="ratA"
                     /locus_tag="ACHKFK_003026"
     CDS             complement(22855..23292)
                     /gene="ratA"
                     /locus_tag="ACHKFK_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO9053312.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMSLVDGPFKKLIGGWKFTPLSQDACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            23463..23945
                     /gene="smpB"
                     /locus_tag="ACHKFK_003027"
     CDS             23463..23945
                     /gene="smpB"
                     /locus_tag="ACHKFK_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO9053313.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24159..24521
                     /gene="ssrA"
                     /locus_tag="ACHKFK_003028"
     tmRNA           24159..24521
                     /gene="ssrA"
                     /locus_tag="ACHKFK_003028"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            24687..>25828
                     /locus_tag="ACHKFK_003029"
     CDS             24687..>25828
                     /locus_tag="ACHKFK_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HFO9053314.1"
                     /translation="MAKIAKKLTDTEIKNTKPAEKEINLFDGDGLMLRIAPLSVGGKK
                     KWYFRYAVPVTKKRTKMSLGTYPHLTLAKARALRDEYLSLLANGIDPQIHNNDKANAL
                     KDATEHTLQAVAKKWLDEKVKTSGISQDHAEDIWRSLERNIFPGLGNLPIKEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPDDLPRFLIALSSASIRLETRLLIEWQLLTWVRPGEAVRARWSDIDQDHRFWNIPG
                     EFMKMKRPHKIPLSKEAMRILELIRPISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRTEVLESALAHTKNNEIIAAYNR"
BASE COUNT         6617 a         6768 c         6046 g         6397 t
ORIGIN      
        1 ctcagcggcg cgggctgtgc ataatacgct ttcccgctac agagtcaagc atttattttt
       61 gctttctctg ccgaagttct caggagaacc ccgctgaccc agcggcgtgt ttgccgttgt
      121 tccgtgtcag tggtggcgca ttatagggag ttattccggc ctgacaagca aaaaatataa
      181 aaactttatt gttcgctcat ttttcaggca aaacatcttt aatatagtct tttccgtcta
      241 acttatagac aaaaacgagc cccgaaggct cgttttatca ttactggacg gcgacgatcc
      301 ggtcttcatt aacttccagg cgaataactt tacccggaac caattcacca gacagtattt
      361 gctgtgccag cgggttttcg atctgctgct ggatagcacg cttcagagga cgtgcaccat
      421 agaccggatc gtaaccgttc tcgctcagca gtttcagcgc ctcgtcagaa atgtggattt
      481 cataaccacg ttcttccaga cgtttgtaca gacgtttcaa ctgaatctgc gcaatcgacg
      541 caatgtgttg ttcacccagc ggatggaaga cgacgacttc atcgatacgg ttaatgaatt
      601 ccggacggaa gttatggctt accacaccga gcaccagctc tttcatgtgc gcataatcca
      661 gttcaccaaa acgttcctga atcagatcag aaccgaggtt agaggtcata atgacgaccg
      721 tattacggaa gtcgaccgtt ctcccctgcc cgtcagtcag acgcccatca tccagtacct
      781 gcaacagaat gttgaagaca tccggatgcg ctttttccac ttcatccagc aggatgacgg
      841 aatacggacg acgacgcacc gcttcggtca gatagccacc ttcttcataa ccaacatatc
      901 ccggaggcgc accaaccaga cgagacaccg agtgtttctc cataaactcg gacatatcga
      961 tacggaccat cgcctcgtcg ctatcaaaca taaagttcgc cagcgcctta caaagctctg
     1021 ttttccccac acccgttggg ccgaggaaca ggaatgaacc aatcgggcga tttggatccg
     1081 ccagccccgc acggctacga cgaatagcgt tagataccgc atcaaccgct tcgttctgac
     1141 caataacgcg atagtgcagt tcttgctcca tacgcagcag tttttcgcgc tcgctttcca
     1201 tcatgcgaga aaccggaatt cccgtccaac gcgccagcac ttcagcaatt tcggcatcgg
     1261 tcactttatt acgcaacaga cgcatagttt tgccttcgag ctgcgttgcg gcttccagtt
     1321 gcttttccag ttccgggatt ttgccgtatt gcagttcaga catccgcgcc aggtcaccca
     1381 cacggcgagc ctgttcaata gcgattttcg cctgttccag ttccgcttta atggtctgcg
     1441 taccagaaag cgaggctttc tctgctttcc actcttcttc taactcggag tactgacgtt
     1501 ctttgtcgct cagttcttcg ttgagcatat ccaggcgttt tttactggct tcatcagact
     1561 ctttcattaa cgcctgttgt tccagtttga gctggatgat acgacgatcg agtcggtcga
     1621 gttcttctgg ttttgagtca atctgcatac gaatgctgga tgctgcttca tcgatcaggt
     1681 cgatggcttt atccggcagc tgacggtcag caatgtagcg atgagacaac gtcgctgctg
     1741 caacaattgc cgggtcagta atttgcacat ggtggtgcag ctcgtaacgt tctttcaggc
     1801 cacgcagaat agcaatggta tcttcaacag aaggctcggc aacaaacact ttctggaaac
     1861 gacgttccag cgcagcatct ttttcgatgt actggcgata ttcgtcaagc gtcgtggcac
     1921 ctacgcagtg caattcacca cgcgccagtg caggtttcag catgtttccg gcgtccattg
     1981 cgccatcggc tttacccgcg ccgaccatgg tatgtaattc gtcgataaac aggatgacgt
     2041 tgccttcctg tttagcaaga tcgttaagta cgccttttaa acgttcttca aactcaccgc
     2101 gatatttcgc cccagccacc agcgcgccca tatccagcgc cagtacgcgg cggcctttca
     2161 acccttcggg cacttcgccg ttgataatac gctgcgccag accttcaacg atggcagttt
     2221 taccgacgcc gggttcacca atcaataccg ggttattttt agtacgacgt tgcagcacct
     2281 gaatggtacg gcgaatttct tcatcacgac caatcaccgg atcgagtttg ccttgttcgg
     2341 ctcgttcggt aaggtcgatg gtatattttt tcaaagcctg acgttggtct tcagcacctt
     2401 gatcgttcac gctttcacct ccacgcattt gttcaatcgc ttgagtaatg ttggcggtgg
     2461 tcgcccctgc tgctttcagg atgtcagcca gcgtgccgcg agactcaagt gccgccagaa
     2521 cgaacagttc tgacgagata aagttatcac cacgtttttg cgccagcttg tcgcaaagat
     2581 taagaacgcg caccagatcc tgtgatggct ggacatcacc accagtacct tcaacctgcg
     2641 gtaaacgatt taatgcctga ttgatatctg tgcgcaactg gccagcattt atgccagcgg
     2701 atgttaataa aggactaacc gaaccccctt cctgattcag cagggcgctc attaaatgaa
     2761 gtggttcgat gaattggttg tcgtgcccga gtgcaagtga ttgggcatcg gcaagagcaa
     2821 gctggaattt attagtaaga cgatccagac gcataactcc tcccataacg gatcaaagtt
     2881 gctactggag attaaatgag gtcatccctc aattattcaa ggttattgac cagattaatg
     2941 tgaaaagaaa atcacgcgta ccggatcgtc ttgattcttt aggttatatc agccaaatga
     3001 aacttgccat acgaccggtg gtcttgtcgc gacgataaga gaagaaagtc tcattttccg
     3061 tatatgtaca acggtcgccg ccgaaaattt gttcaacacc aacgctcgcc agacgctgcc
     3121 gggcaagctg ataaatatcc gccagatact tatcaccatg ctgaatgaaa gctgtactcg
     3181 ctttggcgtc tactgccata aacgcctcgc gaacctccgc ccccacttca aacgcgcgtg
     3241 gaccaattgc cggccctaac caggcgagaa tattttccgg attatcagca aaacaggaaa
     3301 ccgtctcttc cagcacgcct gcgcacagtc cacgccagcc agcatgagcg gcggcgactt
     3361 ccgttcccgt tcgattgcaa aacagcacag ggaggcaatc ggcagtcatc actgcgcaaa
     3421 ccgtgccggg cgtattgcta taagaggcat ctgctcgttt tgaggcataa ggttcaccag
     3481 taagcttaag cacatctttg ccgtgtacct gctcaagcca gaccggttta gaaggcaaat
     3541 tgcccgcagc aaaaagtcgc ttgcgattct cctcaacgtg atccgggtta tcaccacaat
     3601 gagcgccaag gttgagagag tcgtaaggcg gcaagctcac gccgccgata cgggtagagc
     3661 tacaggccgc aacacctttt ggcagcggcc actgcgggac aatcagctta ctcataacca
     3721 gtccacttca tccttatgct cttcgaaatc ggcgcgcatc tcctcaatca gctccaccat
     3781 atcttgtgga ataggcgcat gccattccat ttcgataccg gagatcgggt gataaagacg
     3841 cagcatggtt gcatgaagcg cctggcggtc aaacttacgc agcgtggaga taaacgcttc
     3901 cgaagcacct tttggcggac gcggacggcc accataaacc ggatcgccca ccagcggatg
     3961 agtgatatgg gccatatgca cgcggatctg gtgcgtacgt ccagtttcca gacgcaaccg
     4021 cagacgcgtg tgcacacgga agtgttccat gatgcgatag tgagtgaccg ccggtttacc
     4081 catcggatgc accgccatgt gagtacgttt ggtcgggtgg cggctgattg gttcatctac
     4141 tgtaccgcca gcggtcatat gaccaattgc caccgcttca tactcacgag taatttcacg
     4201 ccgttgcaaa gattcgacta aacgcgtctg agccggaacg gtttttgcca caaccatcag
     4261 gccggtggtg tctttatcca gacgatggac gatgcccgca cgcggtacat cggcaatggg
     4321 cggatagtaa tgaagcaacg cattcagtac cgtaccatcc gggttacccg cgccaggatg
     4381 taccaccagg tcgcgtggtt tattaatgac gatgatatct tcatcttcat agacgatatc
     4441 cagcgggata tcctgcggtt caaaacgcgc ttcttcttca atctcagcgt tgatggccac
     4501 ctgctcgcca cccaatactt tttctttcgg cttatcacaa actttgccgt taaccagcac
     4561 gcgctggtcg aggatccatt cttttattcg cgaacgtgaa taatccggga acatttcggc
     4621 caaagcctga tctaagcgtt gaccgagttg gttttcggac accgttgcag tgagctgtac
     4681 tcgttgtgcc atatatactg cttcttcgtt tatcgttggg gttttacggc tttgccgttt
     4741 aatatagtgt gctattgtag ctggtcttaa ccgggagcag gaacagagaa tctcccgtat
     4801 tacattttga ggaaagtcaa aacgtcatga cgcgcatgaa atatctggtg gcagccgcca
     4861 cactaagcct gtttttggcg ggttgctcgg ggtcaaagga agaagtacct gataatccgc
     4921 caaatgaaat ttacgcgact gcacaacaaa agctgcagga cggtaactgg agacaggcaa
     4981 taacgcaact ggaagcgtta gataatcgct atccgtttgg tccgtattcg cagcaggtgc
     5041 agctggatct catctacgcc tactataaaa acgccgattt gccgttagca caggctgcca
     5101 tcgatcgttt tattcgcctt aacccgaccc atccgaatat cgattatgtc atgtacatgc
     5161 gtggcctgac caatatggcg ctggatgaca gtgcgctgca agggttcttt ggcgttgacc
     5221 gtagcgatcg cgatcctcaa catgcacgag ctgcgtttag tgacttttcc aaactggtgc
     5281 gcggctatcc gaacagtcag tacaccaccg atgccaccaa acgtctggta ttcctgaaag
     5341 atcgtctggc gaaatatgaa tactccgtgg ccgagtacta tacagaacgt ggcgcatggg
     5401 ttgccgtcgt taaccgcgta gaaggcatgt tgcgcgacta cccggatacc caagctacgc
     5461 gtgatgcgct gccgctgatg gaaaatgcat accgtcagat gcagatgaat gcgcaagctg
     5521 aaaaagtagc gaaaatcatc gccgcaaaca gcagcaatac ataacagaaa cctgaaacac
     5581 aaaacggcag ccttgagctg ccgttttttt attctgttag ttgtgaaact gaagcgattt
     5641 agtcactatc gatctcatca aatatggctc gctttgagat attcctcaag taaaaaaaca
     5701 cctcttcctg cgatttctca caaaaaagat tcgttgacaa aaagtgacaa aattatgaga
     5761 tttccatcac acattttgac atcaggaacg ttatgctgaa ttcaccaaga cgggaagaca
     5821 agaggtaaaa tttatgacaa tgaacattac cagcaaacaa atggaaatta ctccggccat
     5881 ccgccaacat gtcgcagacc gtctcgccaa actggaaaaa tggcaaacac atctgattaa
     5941 tccacatatc attctgtcca aagagccaca agggtttgtt gctgacgcca caatcaatac
     6001 acctaacggc gttctggttg ccagtggtaa acatgaagat atgtacaccg caattaacga
     6061 attgatcaac aagctggaac ggcagctcaa taaactgcag cacaaaggcg aagcacgtcg
     6121 tgccgcaaca tcggtgaaag acgccaactt cgtcgaagaa gttgaagaag agtagtcctt
     6181 tatattgagt gtatcgccaa cgcgccttcg ggcgcgtttt ttattgacag cgtgaaaaca
     6241 gtacgggtac tgtactaaag tcacttaagg aaacaaacat gaaacacaca ccgtttttct
     6301 tcgcattctt ttttaccttc ccctgaatgg gaggcgtttc gtcgtgtgaa acagaatgcg
     6361 aagacgaaca ataaggcctc ccaaatcggg gggccttttt tattgataac aaaaaggcaa
     6421 cactatgaca tcggaaaacc cgttactggc gctgcgagag aaaatcagcg cgctggatga
     6481 aaaattatta gcattactgg cagagcgacg cgaactggcc gtcgaggtgg gaaaagccaa
     6541 actgctctcg catcgcccgg tacgtgatat tgatcgtgaa cgcgatttgc tggaaagatt
     6601 aattacgctc ggtaaagcgc accatctgga cgcccattac attactcgcc tgttccagct
     6661 catcattgaa gattccgtat taactcagca ggctttgctc cagcaacatc tcaataaaat
     6721 taatccgcac tcagcacgca tcgcttttct cggccccaaa ggttcttatt cccatcttgc
     6781 ggcgcgccag tatgctgccc gtcactttga gcaattcatt gaaagtggct gcgccaaatt
     6841 tgccgatatt tttaatcagg tggaaaccgg ccaggccgac tatgccgtcg taccgattga
     6901 aaataccagc tccggtgcca taaacgacgt ttacgatctg ctgcaacata ccagtttgtc
     6961 gattgttggc gagatgacgt taactatcga ccattgtttg ttggtctccg gcactactga
     7021 tttatccgcc atcaatacgg tctacagcca tccgcagcca ttccagcaat gcagcaaatt
     7081 ccttaatcgt tatccgcact ggaagattga atataccgaa agtacgtctg cggcaatgga
     7141 aaaggttgca caggcaaaat caccgcatgt tgctgcgttg ggaagcgaag ctggcggcac
     7201 tttgtacggt ttgcaggtac tggagcgtat tgaagcgaat cagcgacaaa acttcacccg
     7261 atttgtggtg ttggcacgta aagccattaa cgtttctgac caggttccgg cgaaaacgac
     7321 gttgttaatg gcgaccggac aacaagctgg tgcactggtt gaagcgttgc tggtactgcg
     7381 caaccacagt ctgattatga cccgtctgga atcacgtccg attcacggta atccgtggga
     7441 agagatgttt tatctggata ttcaggccaa tcttgaatca gcggaaatgc aaaaagcatt
     7501 gaaagagtta ggggaaatca cccgttcgat gaaggtattg ggctgttacc caagtgagaa
     7561 cgtagtgcct gttgatccaa cctgatgaaa aagtgtcgga tgatgtgaat catccggcac
     7621 tggattatta ctggcgattg tcattcgcct gacgcaataa cacgcggctt tcactctgaa
     7681 aacgctgtga gtaatcgccg aaccagtgct ccaccttgcg gaaactgtca ataaacgcct
     7741 gcttatcgcc ctgctccagc aactcaatcg cctcgccgaa acgcttatag taacgtttga
     7801 ttaacgccag attacgctct gacgacataa taatgtcggc ataaagctgc ggatcctgag
     7861 caaacagtcg cccgaccatc gccagctcaa ggcggtaaat cggcgaagag agcgccagta
     7921 gttgctcaag ctgaacattt tcttctgcca ggtgcagccc ataagcaaaa gtagcaaagt
     7981 ggcgcagagc ctgaataaac gccatattct gatcgtgctc gacagcgcta atacgatgca
     8041 accgagcgcc ccagacctga atttgctcca gaaaccattg gtatgcttcc ggcttacgtc
     8101 catcacacca gaccacaact tgctttgcca ggctaccgct gtccgggccg aacatcgggt
     8161 gtaaccccag caccgggcca tcgtgcgccg ccagcatggc ctgtaatggt ccatttttca
     8221 ccgatgccag atcaaccaga atacaatctt tcggtaaagg cggtaatttg ccgataactt
     8281 gctcagtaac gtggatgggc acactaacaa tcaccattcc ggcatcggaa acaatatcag
     8341 ccgctcgatc ccagtcatgt tgctccagaa tccgcacctg ataacccgag agtgtcagca
     8401 tcttctcgaa cagacgtccc atctgaccgc caccaccgac gataaccacc gggcgcagtg
     8461 acggacaaag cgttttaaat cctttgtcgt tttcactgga gtaagattca cgcatcaccc
     8521 gacgcaaaac atcctcaatc agatctggcg gtacacccag agcttccgcc tctgcacgac
     8581 gcgaggccaa catagatgcc tcgcgctccg gaacataaat aggcagtcca aagcggcttt
     8641 tcacctcgcc cacttcagca accagttcca gacgcttcgc taataaattc agcagcgctt
     8701 tatcgacttc atcaatttga tcgcgtaatg cggtcaattc agcaaccata ataaacctct
     8761 taagccacgc gagccgtcag ctgcccgttc agatcctgat gaatttcacg cagcaaggca
     8821 tcggtcattt cccagctaat gcaggcatcg gttacggata caccgtattt catttcactg
     8881 cgcggttgct cggaagactg attgccctcg tggatattac tttcgatcat cagaccaata
     8941 attgagcgat tgccatcttt gatttgagca accacggatt ctgccaccgc aggctgacgg
     9001 cgataatctt tattggaatt accgtggctg caatctacca tcagagacgg gcgcagtccc
     9061 gcctgttcca tctctttttc acattgcgca acatccgcag ggctatagtt tggcgcttta
     9121 ccaccgcgca ggatcacatg cccgtccgga ttcccctgag tttgtagcaa cgcaacctgc
     9181 cctgcctggt taatgccaac aaaacggtgc ggctgggcgg cggcacgcat agcgttaatt
     9241 gccgttgcca gactgccatc ggtgccgttt ttaaaaccaa ccggcatgga aagcccggag
     9301 gccatttcac ggtgagtttg cgattccgtt gtacgagcac caattgctga ccagctaaac
     9361 agatcgccca agtattgcgg gctattcgga tctaacgctt ccgtcgccag tggcagtccc
     9421 atattcacca gctcaagcag caatttacgc gcgatctgca gcccggcttc tacatcaaaa
     9481 gagccatcca tatggggatc gttaattaac cctttccagc cgacagtggt acggggtttt
     9541 tcaaaataga cgcgcattac cagatagagg ctatcgctga cctctgcggc aagggcttta
     9601 aatcgacgag catattccag agcagtttcc ggatcatgaa tggaacaagg accacatact
     9661 accagcagac gaggatcgcg cccggcgata atatctgaaa tggttttacg cgagtcagca
     9721 atctgggctt cttgttgcag gcttaatgga aaagcggcct tcagttgttc cggagtcatt
     9781 aaaacctgtt cgtcggtaat atgtacgtta ttcagcgcgt ctttctgcat gatggcgatc
     9841 ctgtttatgc tcgtttgcga tagttgatcc tcagcgagga tgacgtaacg ataacacata
     9901 aagtaaagtt ttcaatccat atttcgtaca tatttcttta cataagcaat ttattcgcgc
     9961 tttttaccct tattcatgta tatataaatt tacaccatct ataaaaacaa tccgctatgc
    10021 tttgaaaaag gagaaagaaa tgatgaaaaa atttatcgcc cccttgttgg ctttactggt
    10081 tagcggatgt cagattgatc cttatactca cgcgccaacc ttaaccagca ccgactggta
    10141 tgatgttggt atggaagatg cgatatcggg cagcgccatt aaagatgacg atacatttag
    10201 cgattcacag gcggatcgcg gtctatacct taaaggatat gccgaaggac aaaagaaaac
    10261 ttgccagacc gattttactt atgcccgggg actttctggt aaaagctttc ctgccagctg
    10321 caataatgtt gaaaatgcca gccaactgca tgaagtatgg cgaaaagggg ctgatgaaaa
    10381 cgccagcaca atacgattaa attaattacc cctcaaaatc aattaagcat gagattttac
    10441 tcattatgtt ttatttctca cattgatgac gaccgcatga aaggatgata ttttaatgag
    10501 cattctgaaa aaatatcgat gcatttcgag cgaagatggt gaggatccct gaatgcgttt
    10561 ttctcaccga ctgttcttac tgctgattct cctgctgacg ggagcaccaa tactcgcaca
    10621 ggaaccttct gacgttgcga aaaacgtgcg tatgatggtt tccggtattg tcagttacac
    10681 ccgctggcct gggctatcag ggccaccaaa gttatgtatc ttttcatctt cacgttttag
    10741 taccgcctta caagaaaatg ctgctacatc tttaccttat ctgcctgtca tcattcatac
    10801 tcaacaagag gcgataattt caggctgcaa tggtttttat tttggcaacg agtcaccgac
    10861 attccagatg gaattaaccg aacaatatcc gtcaaaggcg ttattattaa ttgccgaaca
    10921 aaataccgag tgcattattg gtagcgcctt ttgtctgatc atccacaata atgacgtcag
    10981 atttgccgta aacctggatg ccttatcgcg tagcggtgta aaggtcaacc cggatgtctt
    11041 aatgctcgca cggaagaaaa atgatggata acgataattc tcttcataag cgccccacgt
    11101 ttaaaagagc attacgcaac atcagcatga ccagcatatt tatcactatg acgctgatct
    11161 ggctgctgct ttccgtgacg tcagtgctaa ccctgaaaca atacgcgcaa aaaaacctgg
    11221 cactgacagc agcaacaatg acttacagtc tggaagcagc tgtcgttttt gccgatggcc
    11281 ctgcagcaac tgaaacactg gcagcgctgg gccagcaagg ccaattttca actgcagaag
    11341 tgcgtgataa gcagcaaaat attctggcat cctggcatta cacccataag gaaccaggcg
    11401 atactttcag caatttcata agccactggc tcttccctgc ccccatcatt cagccgattc
    11461 gccacaatgg tgaaaccatt ggcgaagtac gcttaactgc tcgcgacagt tcaatcagcc
    11521 attttatctg gttttcgctc gctgtcctga ccggttgtat tctgctggca tcaggcatcg
    11581 caattaccct cacccgccat ttgcacaatg gcctggtgga agcgctgaaa aatatcaccg
    11641 atgtcgtaca tgatgtgcgt tctaaccgca atttttcccg acgagtttcg gaagaacgta
    11701 tcgctgagtt tcaccgcttc gctctcgact tcaacagtct gctggatgaa atggaagagt
    11761 ggcagcttcg tttacaggcc aaaaatgcgc agcttctacg taccgcgcta catgacccat
    11821 taaccgggct ggctaaccgc gcagcgtttc gtagcggcat caacacgttg atgaacaatt
    11881 ccgatgcccg aaaaacgtcg gcgttactat ttcttgatgg cgataatttc aaatatatca
    11941 atgataccta gggtcatgcg acgggcgata gagtcttgat tgaaatagca aaacggttag
    12001 ctgaatttgg cgggctgcga cataaagcat accgcctggg cggcgatgaa ttcgctatgg
    12061 tgctctatga tgtacagtcg gaatctgaag tgcagcagat atgctcagca ctgacacaaa
    12121 tttttaatct cccgtttgat cttcataatg gccatcagac taccatgaca ttaagcattg
    12181 gttacgcgat gaccattgag cacgcctctg cggaaaattt acaagagctt gccgatcaca
    12241 atatgtatca ggccaaacac cagcgtgccg aaaagctggt gagataacaa ggatatatcg
    12301 atatgataaa gcacctgcta gcacccctga ttttcacctc gctaatactt actggctgcc
    12361 agtcccctca gggaaagttt actcctgagc aagtcgccgc tatgcaatct tatggattta
    12421 ctgaatccgc cggcgactgg tcgctgggct tatcagatgc cattctgttt gcaaaaaatg
    12481 actacaaatt gctcccggaa agccagcaac agatccaaac catggcagcc aaattggcct
    12541 cgacagggct aacacatgcc cgtatggatg gacacaccga caactatggt gaagacagtt
    12601 acaacgaagt cttatcattg aaacgggcga atgtcgtggc cgatgcatgg gctatgggtg
    12661 gacaaattcc gcgcagcaat ctcaccactc agggtttagg aaaaaaatat cccatagcca
    12721 gtaacaagac cgcccagggc cgcgccgaga accgccgcgt cgcagtggtg attacaaccc
    12781 cttaaaacac gagcaaacag acaaaaaaag ggccagccaa ttggccagcc cttcttaaca
    12841 ggatgtcgct taagcgaaat cttagttaag acgctctttg atacgagcag ccttaccagt
    12901 acgctcacgc aggtagtaca gtttagcttt acgaacagca ccacgacgtt tgacagaaat
    12961 gctgtcaact accggagagt gagtctggaa gacacgctca acgccttcgc cgttggaaat
    13021 tttacgaaca gtgaatgcag agtgcagacc gcggttacga atagcgataa ccacgccctc
    13081 gaatgcctgc agacgttttt tggaaccttc aacaacccat actttcactt ccacggtatc
    13141 acccggacgg aaggaaggta cgtcctgctt catctgctct tgttcaagtt gcttaataat
    13201 gttgctcata atttaatctc ttatcctggg taaactgata tctcgggggc ttacgccatc
    13261 ccatcatgtt tatgttgctg ttgtgcgtgt tccgttttga actccgccag caaccttgct
    13321 tgctcttcag tcagagccag gttttccaga agttcaggtc ttctaagcca ggtacggccc
    13381 agcgactgtt tcaaacgcca gcgacgtatc tcggcatggt tgcccgacag taacactggc
    13441 ggaacttcca tcccttctaa cacctcaggc cgcgtatagt gcgggcaatc cagcaatcct
    13501 tcagcaaagg aatcttccgt tgccgaggct tcatgtccca gtactcccgg aataaaccgg
    13561 gaaacggagt caatcagcgt cattgctggt aactcaccac cactgagaac gtaatcgccg
    13621 attgaccatt cttcgtcaat ttcggtttgg atcacgcgct catctatacc ttcgtagcga
    13681 ccacacacca gaatcaattt ttgattcgtt gccagttcgc tgacgcccgc ttgatcaagc
    13741 ttgcgtccct gtggtgacag ataaatcacc tttgcgcctt cacccgccgc ggcttttgct
    13801 gcatgaatgg cgtcccgcaa gggttgcacc atcattaaca tccccggtcc gccgccgtaa
    13861 ggacgatcgt ccacggtacg gtgccggtca tgcgtgaagt cacgaggact ccagctctgg
    13921 atgctcagca ggccattttt aactgcccgg ccagttaccc cgtaatcggt aattgcgcgg
    13981 aacatttcag gaaacaggct aattatgcca atccacatag cgccgtcttt taccgtttat
    14041 ccggtggttt aaaaaccagg atcccaatct acttcgattg aacgagtagt gagatcgact
    14101 ttcttgataa cctgcccatc gaggaacggt acgagacgtt ccttgatacc aaacgcatct
    14161 ttcaggtttg ccttaatgac gagaacgtca ttagatccgg tttccatcat atctacgact
    14221 ttaccgagat cgtagccttc agtggttact acctggcagc ccatcaggtc tttccagtag
    14281 tagtccccct cttcaagctg aggcaactgc gatgaatcca cgacaatttc acaattcgtc
    14341 agcaggttcg ccgcatcacg atcgtcaacg cctttcagct tgatgatcat gtcctgattg
    14401 tggtgcttcc agctttccag ctggacttgc tgccactgac ccgccttctg gataaaccag
    14461 ggctgatagt caaaaatgct ttcggcgtct tcggtggaag aaaacactct gagccaccca
    14521 cgaataccgt aagacgaacc catttttccc aaaacgatgg gatcaacagg tgcttgcgcg
    14581 gtgagttgtt tgctcatcat gaccaccgtg acagattaag ctgctttgtt tacttctttg
    14641 atcagcgcag caacgcgatc agaaatagtt gcgccctggc caacccagtg agcgatgcga
    14701 tccagatcca ggcgagtgcc ttcttctttt tcgctagcga ttgggttgaa gaaaccaacg
    14761 cgctcaatga agcgaccgtt gcgtgcatta cggctgtcag cgacaacaac ctggtagaac
    14821 ggacgctttt tagcgccgtg acgtgctaaa cgaatagtta ccataacatc ctcttgtgtg
    14881 aataaaacaa ccggacccca tcgaggaacg gagtccggtg tcatattaaa agcccgaaaa
    14941 ttttactgat ttctggagaa aatgcaatca gcagttaata actctgtcgc ataaggtcat
    15001 ttagcgacca ggaaatcccg gcggcatcat ccctttcatg cttctcatca tcttcgccat
    15061 cccgcccttc ttcattttct tcatcatgcg ctgcatgtcg tcgaactgtt tcagaagacg
    15121 gttaacgtcc tgcacctgca taccgcaacc ggcagcgata cgacgcttac gcgaaccttt
    15181 gatgatttct ggcttagcgc gctctttcat cgtcatcgag ttgatgatgg cttccatacg
    15241 caccagcact ttatcgtcca tctgtgactt gacgttatcc gggatctgcc ccatgcccgg
    15301 cagcttgccc atcaggctag ccatgccgcc catatttttc atctggcgca gctgctcaag
    15361 aaagtcgttg agatcgaagc cgtcgccttt tttcagcttg ctggctaatt tctctgcctg
    15421 cgcgcggtca accttgcttt cgatatcttc gatcagtgac agtacgtcgc ccatgccgag
    15481 aatacgggag gcaatacggt ccggatggaa tggctccagc gcctcggttt tctcgccaac
    15541 accgaggaac ttgatcggtt tgccagtgat gtgacgaata gagagcgccg caccgccacg
    15601 agcatcgccg tcgactttag tcagtaccac accggtcagc ggtaacgctt cattgaatgc
    15661 ttttgccgta ttggccgcat cctgaccggt catggcgtca accacaaaca gggtttcaac
    15721 cggattaatc gacgcatgga cttgtttgat ctcgtccatc atcgcttcgt caacgtgcag
    15781 acgaccggcg gtatccacca gcagtacgtc gtagaatttc agtttggctt ctttcagcgc
    15841 cgcgttaacg atatctaccg gcttctgacc aacatcagaa gggaagaaat caacgcccac
    15901 ctgctctgcc agcgtctcaa gctgtttgat tgccgccggg cgatagacgt cggcagaaac
    15961 caccagcact ttcttcttgt gcttctcgcg caggaactta ccgagtttac caacgctggt
    16021 tgttttaccg gcaccttgca ggcccgccat cagcacgacc gcaggcggtt gcgcagccag
    16081 gttcagggtc tggttctctt cgcccatcgc cgcaaccagt tcgttacgga caattttgac
    16141 gaactcctgc cccggcgtca ggctcttatt aacttcatga ccaaccgctt tctcttttac
    16201 gcgattgata aactcacgca ctaccggcag agcgacgtcc gcctccagca gcgccatgcg
    16261 cacttcgcgc agcgtatctt ttacgttgtc ttcagtgagg cgtccacggc cactgatatt
    16321 gcgcagcgtg cgcgacaaac gatcggttaa attatcaaac attgtctctc gcctggggtg
    16381 gaaacggttg gccgcaatcg cgacacatca tcagtatttt gccgcagtat aacatgaagg
    16441 cgtctttgtt gttatgcaac ggttggagca gcgttcacct gacgctatac tgcttctctt
    16501 tcttattgct caaactgtcg acattactat gcccgttttt gctctgctcg cgcttgtcgc
    16561 ctactccgtc agtcttgcgc tgattgttcc cggtctgctg caaaaaaacg gcggctggcg
    16621 gcgcatggct attatttctg cggtcatcgc gctggtctgc cacgcaatcg ctctggaagc
    16681 ccgcatcctg cccgacggtg atagcggaca aaacctcagc ctgctgaacg ttggttcatt
    16741 ggtcagtttg atgatctgta cggtaatgac cattgtggct tctcgcaatc gtggctggtt
    16801 gttgctaccc attgtctatg cctttgcgct tatcaacctg gcgctggcga ctttcatgcc
    16861 caatgaatac atcacccatc tggaagcaac gccggggatg ctggtgcaca ttggcttatc
    16921 gcttttttcc tatgccacgc taattatcgc tgccctgtac gcgctgcaac tggcgtggat
    16981 tgattaccaa ctgaagaaca agaagctggc gtttaaccag gaaatgcctc cattgatgag
    17041 tatcgagcgt aaaatgttcc atatcacgca gattggcgtg gtgctgctaa cgctcactct
    17101 ttgcactggc ctgttctaca tgcacaactt atttagcatg gaaaatatcg acaaggccgt
    17161 gctctctatc gtggcgtggt ttgtctatat tgtgctgctg tggggacatt atcatgaagg
    17221 atggcgtgga cgccgcgtcg tctggtttaa cgttgcgggt gcagtcattc tgacactggc
    17281 ctacttcggc agccgaattt ttcagcagtt aatcagctaa acccagaaaa ggagtttccc
    17341 ctggaacaca tttctactac tacgttgatc attattctga tcatcatggt ggtcatttca
    17401 gcctattttt ccgggtccga aaccggaatg atgactctca accgctatcg tcttcgacat
    17461 atggcgaaac agggtaatcg ctcggccaaa cgcgtcgaaa aattactgcg caagccagac
    17521 cgcctgataa gcctggtgtt aatcggcaat aacctggtca atattcttgc ctccgcgctc
    17581 ggcactattg ttgggatgcg tttgtacggc gatgcgggcg tggcaattgc aactggtgtg
    17641 ctgacttttg tcgtgctggt ttttgctgag gtattgccga aaaccattgc cgcgctgtac
    17701 ccggaaaaag tcgcttatcc gagtagtttt ctactggctc cgctgcaaat tttgatgatg
    17761 ccgctggtct ggttgctgaa tgctattacc cgcatgttaa tgcgcatgat gggcatcaaa
    17821 accgatatcg tggttagcgg ctctttaagc aaagaagagt tgcgcaccat cgtgcacgaa
    17881 tcacgctcac aaatttcccg tcgcaatcag gatatgctgc tgtcggtact cgatctggaa
    17941 aagatgaccg tcgatgacat catggtgccg cgcagtgaaa ttatcggtat tgatatcaac
    18001 gatgactgga aatcgattct gcgccaactc tcccactcac ctcacgggcg catcgtgctc
    18061 taccgtgatt cgctggacga cgccatcagt atgctgcgtg tgcgtgaagc ctggcgactc
    18121 atgtcggaga aaaaagagtt caccaaagaa accatgctac gcgccgcgga cgagatctat
    18181 tttgtcccgg agggtacgcc gctcagcaca cagttggtga agtttcagcg caacaaaaag
    18241 aaagtcggcc tggtcgtcaa cgagtatgga gacattcagg ggctggtgac ggtcgaagat
    18301 attctcgaag agattgtcgg cgatttcacc acgtcgatgt cgccaacact tgccgaagag
    18361 gtcacgccgc aaaacgacgg gtcggtgatt atcgatggca ccgccaacgt gcgagaaatt
    18421 aacaaagcct ttaactggca tctaccggaa gatgatgctc gtactgttaa cggcgtcatt
    18481 ctggaagcgc tggaggagat cccggtagca ggcacccgcg tgcgtatagg cgagtacgat
    18541 atcgatattc tcgacgtaca ggacaatatg attaagcagg taaaagtttt tcctgtaaaa
    18601 ccgctgcgcg agagtgtggc ggagtaacga aaacggcccg gcattcgcat gctgggccgt
    18661 gatttattac gaaagcagaa attaagcttt tgctttcgct acagtcacca tcgccgcacg
    18721 aatcgtacga ccgttcagcg tataaccttt ctgcataatg cccagtacgt tacctggcgc
    18781 aacgtcatca gattccacca ttgcgatggc ctgatgcaca tttgggtcca gtgggacgtt
    18841 ggtttcggcg atcacttcaa cgccaaactt acgcacaaca tccagcatcg acttcagcgt
    18901 cagctcaata ccttcaacca tcgcggacat atccgggtta gctttatcag ccacttccag
    18961 cgcacgatcc aggctatcaa tcaccggcag caattcgttg atgaatttct ccagcgcgaa
    19021 tttgtgagct ttttcaatat ccagttcagt acgacgacgc aggttttcca tttcggcttt
    19081 tacgcgcaaa atgccgtcac gttcacgggt ctgggcttca gccagctgag cttcgagatt
    19141 cgcaattttt tcatcgcgcg gatccacctg ctcagcagaa gcttctggct caactgcctc
    19201 aatctcttcg tgctgatcca tgataatttc ttccggggct tgcccctcag gcgttttctg
    19261 ttctttacta ctcatgaatt tctccgcgtt tttttcgcat tcatctcgct aacttcgctt
    19321 attatgggga tcagtttcag ggtttcaagg gaagcactca cattgtcatc aatcttcgca
    19381 acaaggacct cggaaaaatg aataatcatt tcaagtgtat tggcattgtg ggacacccac
    19441 ggcaccccac tgcactgaca acacatgaaa tgctctaccg ctggctgtgc acaaaaggtt
    19501 acgaggtcat cgttgagcaa caaatcgctc acgaactgca actgaagaat gtgaaaactg
    19561 gcacgctcgc ggagattggg caacaggctg atctcgcggt tgtcgttggc ggcgacggta
    19621 atatgctggg cgcggcgcgc acgctcgccc gctacgatat taaagttatt ggaatcaacc
    19681 gtggcaacct gggtttcctg actgaccttg atcccgataa cgcccagcaa caattagccg
    19741 atgtgctgga aggccactac atcagcgaga aacgtttttt gctggaagcg caagtctgtc
    19801 agcaagattg ccagaaacgc atcagcacgg cgattaacga agtggtactt catcctggca
    19861 aagtagcgca tatgattgag ttcgaagtgt atatcgacga gatctttgcg ttttcgcagc
    19921 gttccgatgg actgattatt tcgacgccaa caggctccac cgcatattcc ctctctgcgg
    19981 gcggtcctat tctgacaccc tctctggatg cgattaccct ggtgcccatg ttcccgcata
    20041 cgttgtcagc acgaccactg gtcataaaca gcagcagcac gatccgtctg cgtttttcgc
    20101 atcgccgtaa cgacctggaa atcagttgcg acagccagat agcactgccg attcaggaag
    20161 gtgaagatgt cctgattcgt cgctgtgatt accatctgaa tctgattcat ccgaaagatt
    20221 acagttattt caatacatta agcaccaagc tcggctggtc aaaaaagtta ttctaatttt
    20281 acgccagcct ctttactgta tataaaacca gtttatactg tacacaataa cagtaatggt
    20341 ttttcataca ggaaaacaac tatgttggca caactgacca tcagcaactt tgctatcgtt
    20401 cgtgagcttg agattgattt tcatagcggc atgaccgtaa taactggcga gaccggcgcg
    20461 ggtaaatcta ttgcaataga tgccctcggc ctttgtctcg gtggtcgcgc tgaagccgac
    20521 atggtgcgta ccggcgctgc tcgtgccgac ctgtgcgccc gtttttctct gaaagatacg
    20581 ccagcggccc tgcgttggct ggaagaaaac cagcttgaag acgggcatga atgtttgctt
    20641 cgtcgcgtaa tcagcagcga tggtcgctcc cgtggtttca tcaacggtac agctgttcct
    20701 ctgtcacaac tgcgcgaact gggtcagttg ctgattcaga tccatggtca gcacgctcat
    20761 cagttactca ccaaacccga gcaccaaaaa ttcctgcttg atggctatgc caatgaaacc
    20821 tctcagctcc aggaaatgac cgcacgttat catttgtggc atcaaagctg ccgtgacctc
    20881 gcgcatcatc aacagttaag tcaggaacgc gccgctcgtg cggagctgct gcaataccaa
    20941 ttaaaagaac ttaacgaatt taatccgcag cctggagagt ttgagcaaat cgacgaagag
    21001 tacaaacgtc tggcgaacag cggtcaattg ctgaccacca gccagaatgc attggcatta
    21061 atggctgacg gtgaagacgc aaacctgcaa agtcaacttt acacggctaa acaactggtg
    21121 agcgaattga ttggcatgga cagcaaactg tccggcgtac ttgatatgct ggaagaggct
    21181 accatccaga ttgctgaagc cagtgatgaa ctgcgccact actgcgatcg tctggatctc
    21241 gatcccaacc gactgtttga acttgaacag cgcatctcaa aacagatttc gctggcacgt
    21301 aaacatcacg tcagccctga ggcattgcca cagtattacc agtcgctact ggaagaacag
    21361 cagcaactgg acgatcaggc cgactcacaa gaaacgctcg cgctggcggt aacgaaacat
    21421 catcagcagg cactggaaac ggcgcgcgcg ttacaccagc aacgccagca ttatgcaaat
    21481 gagttggcac agctgatcac cgatagtatg catgcactct ctatgccaca tgggcagttt
    21541 acgatcgatg ttaaatttga cgagcatcac ctgggcgctg acggcgctga tcgtattgag
    21601 tttcgggtaa ccaccaaccc aggtcagcca atgcagccta ttgccaaagt cgcatccggt
    21661 ggtgagttgt cccgcatcgc attggcaatc caggtcatta ccgcgcgtaa aatggaaacc
    21721 ccggcactga tttttgatga agtggatgta gggattagcg gcccaacagc ggcagttgtc
    21781 ggcaaactgc tacgtcaact tggcgaatca actcaggtga tgtgtgttac ccacctgcca
    21841 caagtcgcgg gatgtggtca tcaacactat tttgtcagca aagaaaccga tggtgcgatg
    21901 acagaaacgc atatgcaatc cctggataaa aaagcgcggt tacaagagtt ggcgcgcctg
    21961 cttggtggca gtgaagtcac acgtaacaca ctggcgaatg cgaaagaact gcttgcggcg
    22021 taaacttttt tcctgcttca cggtcagagt aaacagcaaa acgccgtaag accggaaagc
    22081 aaaaggtttt aaagtgatga aaggtctatt atcatcggca tattacagat gagccacgta
    22141 ctgctcgggc ccgaaaagga atcaaatcac tatgcgctgt aaaacgctga ctgctgcagc
    22201 agcagtacta ttgatgttga ccgcaggctg ttccactctg gagcgagtgg tttaccgtcc
    22261 tgacatcaac caggggaact atctgaccgc taacgacgta tccaaaatac gtgttggcat
    22321 gacgcaacaa caagttgcgt acgcattggg tacaccgctg atgtccgatc catttggtac
    22381 aaatacctgg ttctatgtct tccgccagca acctggtcat gaaggtgtaa cgcagcaaac
    22441 gctgacgctg acctttaaca gtagcggtgt gttgaccaac attgataaca aacctgcgct
    22501 gagtggtaac taataataaa gttgctctca aagacgttaa aaaaggtgct caatgagcac
    22561 cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg aagctctttc
    22621 ggatcggcaa tgagaggacg ataaatctcc acccgatcgc catcatgcac aatatcgctg
    22681 agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat atcggtacgc
    22741 aattccagca aaccactggc gcgaatagct tcttcaaccg tcgcgccctc ctgcaacgtc
    22801 acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc aattttacct
    22861 ggcactgtag acctcttttg cacgaacagt aaaagcctgc accatattag ctgccagctc
    22921 tttaaacaca cgaccaaagg cgagttcaat caacttattg gtaaactcaa agtcgagatg
    22981 aaactcaata cggcacgcgt cctggctcaa cggcgtaaac ttccaaccgc cgatcaattt
    23041 cttaaacggt ccatcaacca gactcatgag aatactttga ttactggtca actggttgcg
    23101 ggttgtaaac gttttgctga tcccagcctt agacacatct accgcagcag tcatctgccc
    23161 aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag gataagactg
    23221 aacgtcattc actaactgat acatttgctc cgcgctgtaa ggcaccagtg cggtccggct
    23281 aatctgaggc ataacaattt ccatcaacaa aaatccaaca aataatatca tttatcctgc
    23341 tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag atagagcctt
    23401 gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga ttcacgatgc
    23461 ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt aacaagcgcg
    23521 cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg caaggctggg
    23581 aggttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctatgtc cttctgcgcg
    23641 acggagaggc atttctgttt ggcgctaaca tcacgccaat ggctgtggcc tccacgcatg
    23701 tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa ctggactcat
    23761 tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg tactggaaaa
    23821 atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa gaaacagcac gataaacgtt
    23881 cagatatcaa agaacgcgaa tggcaggtgg ataaagcgcg tatcatgaaa aacgcccacc
    23941 gttaaaccta tataagcgat actaatcaaa tcctcaccgc gcctccctat ccggtggcgc
    24001 gaatgaacat cttatcgact gtcaaatctg acacagatgt agccatccca ttgcttaatc
    24061 gaaaaaaatc atactatgca ggcgctaatt ccaggatgat aacgcctttt gggctagtca
    24121 tgctgctcat aaatctggta tacttaccgt tacacattgg ggctgattct ggattcgacg
    24181 ggatttgcga aacccaaggt gcatgccgag gggcggttgg cctcgtaaaa agccgcaaaa
    24241 aatagtcgca aacgacgaaa actacgcttt agcagcttaa taacctgctt agagccctct
    24301 ctccctagcc tccgctctta ggacggggat caagagaggt caaacccaaa agagatcgcg
    24361 tggaagccct gcctggggtt gaagcgttaa aacttaatca ggttagtttg ttagtggcgt
    24421 gtccgtccgc agctggcaag cgaatgtaaa ggctgactaa gcatgtagta ccgaggatgt
    24481 aggaatttcg gacgcgggtt caactcccgc cagctccacc actttttagt tgtttgaagt
    24541 atacttaagt ctactaagcc cgcatggaac cagccttgcg ggctttttta cgtctacaga
    24601 agtctactga gatttgctag aagctactcg ttatggcacc ctttttgaga cccaatgtaa
    24661 agggtcccaa aaccgagggt cctaaaatgg caaagatcgc taagaaactt actgacactg
    24721 aaatcaaaaa taccaagcca gcagaaaagg agatcaacct ttttgatggt gacggcctga
    24781 tgctacgaat cgcccctctc tctgtaggcg gcaagaaaaa atggtatttc aggtatgcag
    24841 tgccagtaac caaaaagcgg accaagatga gcttaggaac ctatcctcat ctcacacttg
    24901 ccaaggctcg ggctttacgt gatgaatact tgtcgttgct tgcaaatggt atcgatccgc
    24961 agattcacaa caacgataaa gccaacgccc ttaaggatgc cactgagcac acgctacagg
    25021 cagttgctaa gaaatggtta gatgagaagg ttaagacctc tggtatctct caagaccacg
    25081 cagaagacat ctggcggagc cttgaacgaa atatcttccc cggtttaggc aaccttccta
    25141 tcaaagagat ccgtcccaag ctcttaaaac aacatcttga ccccatagaa caacgaggcg
    25201 tattggaaac cctgcgccgc atcatttcac gtctaaatga aattttccgt tgggctgcga
    25261 cagaagaact tattgagttt aatcctgctg acaatctcgg ccagagattc agcaaaccca
    25321 agaagcaaaa tatgccagct ttaccaccag atgatttacc tcggttcttg attgctctat
    25381 ctagcgcttc tatacggctc gaaacacgtc ttctaattga gtggcaactg cttacgtggg
    25441 ttcgtccggg agaagctgtc cgcgctcgtt ggagtgatat agaccaagat catcgattct
    25501 ggaacatccc cggtgagttt atgaagatga agcgtcctca caagattccg ctgagtaaag
    25561 aggctatgcg tatacttgag ttaatcagac caatcagtgg tcatagagag tgggttttcc
    25621 caagcattaa ggccccgctt aaccacatgc atgagcaaac agctaatgct gcaatcatcc
    25681 gtatgggatt cggtggtgaa ctggtagccc acggaatgcg ttctatagca agaacggctg
    25741 ctgaagaaag tggtaaattt agaacagagg tgcttgaatc agccttagct catactaaaa
    25801 acaatgaaat catagccgct tacaatcg
//
LOCUS       DAZGJT010000068        25679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZGJT010000068 DAZGJT010000000
VERSION     DAZGJT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.068"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..648)
                     /gene="queE"
                     /locus_tag="ACHKFK_003030"
     CDS             complement(<1..648)
                     /gene="queE"
                     /locus_tag="ACHKFK_003030"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO9053315.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKEDATRLCIDTCIARNWRLSMQT"
     gene            821..1423
                     /locus_tag="ACHKFK_003031"
     CDS             821..1423
                     /locus_tag="ACHKFK_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HFO9053316.1"
                     /translation="MFRIFIAFIFIFNLSGCGYNDIQTYDEQVNASWSEVLNQYQRRT
                     DLIPNLVASIKGYSSHEQEVLEAVTLARSQANRASSDLQKTPGDEQKLQAWQQAQAQV
                     TRTLGQLTIISERYPQLKAQELYQNLMVQLEGSENRIAVARGRYIKAIEQYNVTIRKF
                     PAVLTAKVMDYTPKKNYLPDDVTAVSKAPTIDFSQNANAH"
     gene            1437..2108
                     /locus_tag="ACHKFK_003032"
                     /pseudo
     CDS             1437..2108
                     /locus_tag="ACHKFK_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311662.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            2345..3499
                     /locus_tag="ACHKFK_003033"
     CDS             2345..3499
                     /locus_tag="ACHKFK_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9053317.1"
                     /translation="MRIFLLLLMLCGFQVFAERLPSVTDLTGTLTTEEQSALNQQLQT
                     LEQQNHFQVAVLVTPTTGGKNIKLAASQRYGVYHWKPVGEKRYGEGILILVVWPEGLA
                     SMKIGHGLEQMLPPEQAAQIVRYHMQPEFEKNNLFAGLTGGIESIAQFTHIKANLSPL
                     DALANHLFANPQLSLPCLAWTVLMIVAIIVLWRFTSRPGPGIWMISMITPTVWFFCFQ
                     DDVIIRRVSVVCFSLFFAAICWQRLAVVFNMCRVFPMMLAPKNKNAKVKKQKKTPQFR
                     ESGRNSLFVVMLIIIVGWCLVFASNKDIAFISTIIGLIDHFIGPITIAGIVLLIIVAK
                     FTGNLKLGSGEKSNRKTGNRNAHAHSRSSSRNSFRGGGGSSGGGGSSDRW"
     gene            3505..4377
                     /locus_tag="ACHKFK_003034"
     CDS             3505..4377
                     /locus_tag="ACHKFK_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO9053318.1"
                     /translation="MRLIFILFTLWCLPGLAQQIAVPELRQQVTDITGTLSTSEQQSL
                     TQQLQDITHKTRAQVAVLVVPSTGDDSIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAKGLELAIIALNNRLTSQHQY
                     SVNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSSVCISAIYLLDL
                     FLFSDKTFSIAVFFFFFTFTTIMVFTCLCVLQKKASGRSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(4437..5735)
                     /gene="eno"
                     /locus_tag="ACHKFK_003035"
     CDS             complement(4437..5735)
                     /gene="eno"
                     /locus_tag="ACHKFK_003035"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO9053319.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(5823..7460)
                     /gene="pyrG"
                     /locus_tag="ACHKFK_003036"
     CDS             complement(5823..7460)
                     /gene="pyrG"
                     /locus_tag="ACHKFK_003036"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO9053320.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(7688..8479)
                     /gene="mazG"
                     /locus_tag="ACHKFK_003037"
     CDS             complement(7688..8479)
                     /gene="mazG"
                     /locus_tag="ACHKFK_003037"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO9053321.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(8551..8886)
                     /gene="mazF"
                     /locus_tag="ACHKFK_003038"
     CDS             complement(8551..8886)
                     /gene="mazF"
                     /locus_tag="ACHKFK_003038"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO9053322.1"
                     /translation="MVSRYVPDTGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(8886..9134)
                     /gene="mazE"
                     /locus_tag="ACHKFK_003039"
     CDS             complement(8886..9134)
                     /gene="mazE"
                     /locus_tag="ACHKFK_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO9053323.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(9212..11446)
                     /gene="relA"
                     /locus_tag="ACHKFK_003040"
     CDS             complement(9212..11446)
                     /gene="relA"
                     /locus_tag="ACHKFK_003040"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO9053324.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(11494..12795)
                     /gene="rlmD"
                     /locus_tag="ACHKFK_003041"
     CDS             complement(11494..12795)
                     /gene="rlmD"
                     /locus_tag="ACHKFK_003041"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO9053325.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LECFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVAPEDCVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            12852..15608
                     /gene="barA"
                     /locus_tag="ACHKFK_003042"
     CDS             12852..15608
                     /gene="barA"
                     /locus_tag="ACHKFK_003042"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO9053326.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPNMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNICQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(15848..17188)
                     /gene="gudD"
                     /locus_tag="ACHKFK_003043"
     CDS             complement(15848..17188)
                     /gene="gudD"
                     /locus_tag="ACHKFK_003043"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO9053327.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGTYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPLEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(17209..18513)
                     /gene="gudX"
                     /locus_tag="ACHKFK_003044"
     CDS             complement(17209..18513)
                     /gene="gudX"
                     /locus_tag="ACHKFK_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO9053328.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVKAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALNVPVCELLGPGKQRDAITVLGYLFYIGDRTKTDLPYLENTPGNHE
                     WYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDTVRALKKRFPDAR
                     ITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAEFRRATGLPVATN
                     MIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLTWGCHSNNHFDIS
                     LAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVPDAPGLGVELDWE
                     QVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(18515..19867)
                     /gene="gudP"
                     /locus_tag="ACHKFK_003045"
     CDS             complement(18515..19867)
                     /gene="gudP"
                     /locus_tag="ACHKFK_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO9053329.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(20301..20750)
                     /gene="yqcA"
                     /locus_tag="ACHKFK_003046"
     CDS             complement(20301..20750)
                     /gene="yqcA"
                     /locus_tag="ACHKFK_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO9053330.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEHWGTMLS"
     gene            complement(20768..21550)
                     /gene="truC"
                     /locus_tag="ACHKFK_003047"
     CDS             complement(20768..21550)
                     /gene="truC"
                     /locus_tag="ACHKFK_003047"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO9053331.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGELLA
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(21550..21879)
                     /gene="yqcC"
                     /locus_tag="ACHKFK_003048"
     CDS             complement(21550..21879)
                     /gene="yqcC"
                     /locus_tag="ACHKFK_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO9053332.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPLPHQFASTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(22501..23046)
                     /gene="syd"
                     /locus_tag="ACHKFK_003049"
     CDS             complement(22501..23046)
                     /gene="syd"
                     /locus_tag="ACHKFK_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO9053333.1"
                     /translation="MDDLTARALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     IWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23114..23962
                     /gene="queF"
                     /locus_tag="ACHKFK_003050"
     CDS             23114..23962
                     /gene="queF"
                     /locus_tag="ACHKFK_003050"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO9053334.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAIGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNNDFVPST
                     TRLVRQ"
     gene            24074..25438
                     /gene="ppnN"
                     /locus_tag="ACHKFK_003051"
     CDS             24074..25438
                     /gene="ppnN"
                     /locus_tag="ACHKFK_003051"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO9053335.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         6376 a         6743 c         6096 g         6464 t
ORIGIN      
        1 tgtctgcatc gacaaacgcc agttgcgcgc aatgcaggta tcgatacata aacgcgtagc
       61 atcttccttc tggctaattg gctgaagtgc aatgactcgc ggtttatcat cggtcagcgt
      121 cgccagcaat tcatccagtg cttcaatatc acgtacgcgt cctactgggt gtttgatttc
      181 gttggctcgc tccagggctt gtgacaacac ttcatagcca ccgcgcatat ttagctttgg
      241 tgatacggta acccaggtat tcggtgtgca gcgtacttca tgagtaccac tagtttcgat
      301 ctggcagcta aaaccgttct tttcgagcag gtcagtcagt ggcagcaaat catgaatgca
      361 aggctcacca cccgtaatca ccacatgacg cgcggtgtat ccctgacggc caatgacagc
      421 cagcaaatct tcactgctcg ccgcccccca cttatcactc tctttggtct tcgccagaat
      481 gctgaaaagg gagacttccc gatcctcaag cttttcccag gtgtgtttgg tgtcgcacca
      541 ggcacagcca accggacatc cctgtaaacg aataaaaatg gcggggacgc cggtaaagta
      601 accctcacct tgcagggtct ggaacatctc gttaatcggg tactgcatag cattctctgt
      661 gaagtggata attgttaatt attgcagatc ctgccacaac aatcatgtct tattaacatt
      721 ctgttacagg caggtttgat gcaaaaattg gctcagatca ttaagaataa aatgaagcgg
      781 cgtattatgt tttcaaggat taaatgaaag ggatttaaac atgtttagaa tattcatcgc
      841 gtttatattt attttcaatt tatcaggttg cggttataac gatattcaaa cctatgatga
      901 acaggttaat gcctcctggt cagaagtatt aaatcaatat caacgtcgca ccgatcttat
      961 tcctaatctt gtcgctagta ttaaaggtta ctccagccat gaacaagagg tactagaagc
     1021 cgtaacattg gcacgtagtc aggctaatcg cgccagtagc gatcttcaga aaactccggg
     1081 agatgaacaa aaattacaag cctggcaaca ggcgcaggcg caggtgactc gcactctcgg
     1141 gcagttaacg attattagtg agcgttaccc tcagttaaag gctcaagagc tctatcaaaa
     1201 cttaatggta cagctcgaag gaagtgaaaa tagaattgcc gttgcgcgag gaagatatat
     1261 taaagccatt gaacaatata atgtcactat ccgcaaattc cccgctgtat taaccgcaaa
     1321 ggtcatggat tacacaccaa agaaaaatta tttgccggat gatgtcaccg cagtaagtaa
     1381 agccccaaca atagatttta gccagaatgc taatgctcat taagcgggga cgttctatgc
     1441 gaaattttat cttccttatg gctttcttct gttcatctgt gtttgctaca caaattccag
     1501 tacctgaatc acccaggtat gtgaatgact taaccggcac attaacaaac agcgaagtaa
     1561 acaccctgac caaccagatt aaagccctga cgcaaaaaaa ccacgcacag ttggtcgtgt
     1621 tagtggttga tacaacaggc gatgaaacca tcgagcagta cgcaacgcgg gtattcgata
     1681 gctggaaacc gggtgataaa gaccgtgatg atggcgttct actgttagtc gcctggcagg
     1741 atcataccgt gcgtattgaa atcggttacg ggctggaagg tattattacg gatgcacagt
     1801 ccgggaaaat tattcgcaac agtattattc cggcatttaa aaaaggcgac ctggctggtg
     1861 gtttacaaaa ggggatcaac gatattgaaa gtcgcctgac ggggaataat tcagcaacga
     1921 taacaccgac cgatcaccct ttgcccttta gcggttggtg ggctttatta gtctgggcga
     1981 taatactgac gtttatttct gccagaggat atatcaaaac gttgggagta atttgcttcg
     2041 cagccattgt actggccttt gttttaccta tagcaggttt cagcggaagc tggggagtac
     2101 tcacaacatt actctgcttc gccacaccgt ttctggcagt tgccatcgct tttaccccct
     2161 tcggcaaaaa ggttcgggat tcgatgcgtg atgccaacca gccatcacgc catacaagaa
     2221 gcaattcttc ctcccactat tcatcatcgt catcctcttc attcgacaat gacaatttta
     2281 gcggtggtgg cggttcatct ggtggcggtg gcgcttccgg gcgctggtaa caaaaggaat
     2341 tgatatgcgt atttttctct tacttctgat gctatgcggt tttcaggtat tcgctgaacg
     2401 attgccttca gtgactgact taaccgggac attaacgaca gaagaacaaa gtgctttaaa
     2461 tcaacaatta cagactctcg agcagcaaaa ccacttccag gtggccgtgt tggttacgcc
     2521 gacaacaggg ggcaaaaaca ttaaacttgc agcctcgcag agatacgggg tatatcactg
     2581 gaaacccgtt ggagagaagc gatatggcga aggtatctta attctcgtcg tttggccgga
     2641 agggctggcg tcaatgaaga ttggacatgg actggaacag atgttgccgc cggaacaagc
     2701 tgcgcaaatt gttcgttacc atatgcagcc agaattcgaa aaaaacaatc tctttgctgg
     2761 attaacgggc ggaattgaaa gcatcgcaca gtttacccat atcaaagcga accttagccc
     2821 tttggatgct ttagccaatc atctgtttgc taatccgcaa ctttcgctgc catgccttgc
     2881 ctggacagtg ctgatgatcg ttgccatcat tgtcctctgg cgatttacca gccgtccagg
     2941 acctggcata tggatgatat caatgattac acctacggtc tggtttttct gctttcagga
     3001 cgatgtcatc attcggcggg tgtcggtcgt ttgttttagt ttatttttcg cagcaatctg
     3061 ctggcagcgt ctcgcagtag tttttaatat gtgccgcgta tttcctatga tgctggcccc
     3121 aaaaaataaa aacgctaaag tgaaaaaaca aaagaagact ccgcaatttc gtgaatcagg
     3181 acgaaattca ttgtttgtcg tgatgctgat aattattgtg ggatggtgcc tggtgttcgc
     3241 cagtaataaa gatattgcat tcatctcaac aattattggg ttaattgatc attttatcgg
     3301 cccaataacc attgctggca tcgttctgct gatcattgtg gccaaattta ctggcaatct
     3361 taagctgggg tcaggagaaa aaagcaacag gaaaacgggg aatcgtaatg ctcatgctca
     3421 ttctcgctct tcgtcgagaa attctttccg cggtggtggc ggttcttcag gaggcggcgg
     3481 ttcatccgac cgttggtgat cgtaatgcgc ttaatcttta ttctattcac tttgtggtgc
     3541 cttcctgggc ttgcccagca aattgctgtg ccggaactgc gccaacaggt gaccgatatt
     3601 actggtacgt taagcacttc tgaacaacaa tcactgacac agcaactgca ggatattaca
     3661 cataaaacac gggcgcaggt cgctgtatta gtagtgccgt ccaccggaga cgattccatt
     3721 gaacaatatg ctacaagggt ttttgacaat tggcgtttag gagatgccaa acgtaatgat
     3781 gggatactga tcattgttgc ctggtcggat cgcactgtcc gcatccaggt aggttatggg
     3841 ctggaagaaa aggtaaccga cgctctggct ggagatatca tccgtagcaa catgataccc
     3901 gcatttaaac aacaaaaatt agctaaggga ttagagttag ctataatcgc tttgaataac
     3961 cgactcactt cacaacacca atattcggtt aatccttcag aaagtgaatc agcatcttcc
     4021 agcgatcatt actactttgc tattttttgg gtatttgcag tgatgttctt ccctttctgg
     4081 ttttttcatc aaggcagtaa tttttgtcgc gcatgtaaaa gcagtgtctg tatttcagca
     4141 atctatcttt tagatttatt cctgttctcc gataaaactt tttccattgc tgtatttttc
     4201 ttctttttta ctttcaccac cattatggtc tttacctgtt tatgtgtact tcagaaaaaa
     4261 gcatctggta gaagttatca ttcagacaac agcggttccg gtggtggctc aagtggcggc
     4321 ggatttagcg gcgggggcgg ttcttctggc ggtggcggcg cgtccggtcg ctggtaactc
     4381 aaccataaaa aatgccagcc cgaaggctgg catttttaaa tcagataaag tcagacttat
     4441 gcctggcctt tgatctcttt acgaccgttg tacggtgctt tttcgcccag agcttcttcg
     4501 atacgaatca gctggttgta tttagcaaca cggtcagaac ggctcataga accagttttg
     4561 atctggcctg cagcagtacc aacagccagg tcagcgatgg tagcgtcttc agtttcgcca
     4621 gaacggtgag agataactgc agtgtagcca gcgtctttcg ccatcttgat tgcagccaga
     4681 gtttcagtca gagaaccgat ctggttgaat ttgatcagga tggagttagc gatacctttt
     4741 tcgatacctt ctttcaggat cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg
     4801 attttgtcgc ccagaacttt ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga
     4861 ccgtcttcga tggaaacgat cggatactgt ttggtcaatt cttccaggaa gtgagtgaat
     4921 tcttcagagg tgaacgcttt gttgccttcg ccagccagaa cgtatttacc gtctttgtag
     4981 aattcagaag ctgcgcagtc catcgccaga gtgatgtctt tgcccagttc ataaccagca
     5041 gctttaacag cttcagcgat aacagccaga gcttcagcgt tggaacccag gttcggcgca
     5101 tagccacctt cgtcaccaac agcagtgttc atgcctttcg ctttcagaac ttttgccagg
     5161 tgatggaaaa cttcagaacc catgcggatg gcttctttca cagttttcgc gccaaccggc
     5221 tgaatcatga attcctggat atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg
     5281 ttcatcatcg gaaccggcat agagtatttg cccggagtgc cgttcagttc agcgatgtgc
     5341 tcgtacagcg gcataccttt agcagctgca gcagctttgg cgttagccag agatacagcc
     5401 aggattgcgt tcgcgccgaa tttggatttg ttttcggtgc cgtccaggtc gatcatgatc
     5461 ttgtcaatgc cagcctgatc tttagcatct ttgccaatca gcgcctgagc gatcgggccg
     5521 tttaccgcag caacagcttt ggttacgcct ttacccagga aacgggattt gtcgccatcg
     5581 cgcagttcca gagcttcacg ggaaccagta gaagcacctg acggagcagc tgccataccg
     5641 acgaaaccac cctccagatg tacttcggct tcaacagtcg ggttaccacg ggagtcgatg
     5701 atttcacgac cgatgatttt tacgattttg gacattaggt tttcctcaag tcactagtta
     5761 aactgaaact ccagacaaac aacgcgtacc cagggtacgc gttgccgttc taactttttt
     5821 acttacttcg cctgacgttt ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc
     5881 gggtgaccat cacgtggagt agaagtaaac tccggatgga actggcaagc cacgaaccac
     5941 ggatgattcg gaacttcgat gatctcgacc aactgatcat ccccggaacg gcccgcaacg
     6001 cgcagacctg catcttcaat ctgtttcaac agcatgttgt tgacttcgta acggtgacga
     6061 tgacgctcaa caattgtcgg cgcattgtac agttggcgaa ccaggctatc gtcaaccaac
     6121 tggcactgct gtgcgccgag acgcatggta ccgccgagat cgctcttctc gctacgaact
     6181 tcaacgttgc cgttttcatc gcgccactcg gtaatcagcg ccacaaccgg gtacttacag
     6241 tctggcacaa attccgtaga gttggcgttc tccatgttgg caacatggcg agcgtaatca
     6301 attaacgcca cctgcatacc caggcaaatg cccagataag gaatattgtt ctcacgcgca
     6361 aaacgcgcgg tcgtaatcat gccttctacg ccacgatagc cgaaaccgcc aggtacgagg
     6421 attgcgtcca gacctttgag gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc
     6481 agtttgatgt tgacgctgac acgattcttc agcccaccgt gtttcagtgc ttcgatcacg
     6541 gatttgtatg catccggcag ctcaatgtat ttgccgacca taccaatggt gacttcactt
     6601 accggattcg cttcttcgaa gataacctgt tcccattcgg acagattcgc ttccgggcag
     6661 tttaagctga atcgtttaca aatataatcg tccagcccct gagatttcaa caggcccgga
     6721 attttataga tggaatcgac gtctttcaga gaaataaccg ctttttccgg aacattacag
     6781 aacaatgcaa tcttcgcacg ttcgttcgcc ggaacggcgc gatctgaacg acaaatcagg
     6841 atatcaggct ggataccgat tgaaagcagc tcttttacag agtgctgagt cggtttggtt
     6901 ttgacttcac ctgacgctgc catgtacggc accagcgtca ggtgcataaa cagggtgtgc
     6961 tcacggccaa tttcaacagc catctggcga atcgcttcga ggaacggcag ggattcgata
     7021 tcaccgacag taccgccgat ttcgaccagt actacgtcat gaccttcgcc gccttccagc
     7081 acgcgctctt tgatagcgtt agtgatgtgc ggaataacct gtacggttgc gccgagatag
     7141 tcaccgcggc gttctttacg cagaacgtca gagtagatac gacccgtggt gaagttgttg
     7201 cggcggctca ttttggtacg aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca
     7261 gcgccgtctt cagtaacgaa cacttccccg tgttggattg ggctcatagt acctggatcg
     7321 acgttgatgt acggatccag tttcatgatg gtcacattga ggccacgggc ttcaagaatg
     7381 gctgcgaggg aggctgcggc aatgccttta cccagagagg atacgacccc gccggtcaca
     7441 aaaatatagt tcgttgtcat gctgaacctg agaagttagg ttgaaaagac gatggaataa
     7501 ccaggacggg aaagcagtat acccgaacat gacctgtgcc acaaactttc attatccctc
     7561 ctcttcgcca gcgcactatt gaaatcagga gtgagaaaat agcccctttg gggtaaatgt
     7621 ttttgacgca aatcaagcgc ttgtcattta aaaaatgaca caaatcgcgc ttgaccgcgt
     7681 aattccctta gagatcaatt tcctgccgtt ttacctgttg ccagacttct tccattgttt
     7741 cgaggtcaac acctgtcatt tccagtccac gcgcggcaac aatacgctcc acttcgcgaa
     7801 aacgacgctc gaatttttcg ttcgcttttt gcaatgcgat ttccgctttt gtccctaaat
     7861 gacgagccag attgaccgtg gcaaacagca ggtcacccat ttcctcctcc agtttagcct
     7921 ggtcgacaac agcctgccgc gcctcgtaca tcacctcgtc gatctcttcg tagactttat
     7981 cgactaccgg accaagcgtc gtccagtcga aaccaacgtt ggcgcaacgt ttctggattt
     8041 tttgcgcacg cattaaagcc ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg
     8101 ctttctgcgc gcgttcttcg gttttgattt gctcccaacg ggcaagcact tcactactat
     8161 tttcggcaga actgtcagca aaaacatgcg gatggcgacg ctctaattta tcgctaatag
     8221 cagcgcaaat atcattaaag tcaaagcgcc cttcttcctg agccatttgt gcgtaaaaca
     8281 ccacctggaa cagcagatcg cccagttcac cgcgcagatc gtcaaaatct tcacgggcga
     8341 tggcgtccag cacctcgtag gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct
     8401 gctctttatc ccacgggcag ccgttttccg ggtcgcgcag gcgctgcatg atggtgagca
     8461 aacggtcgat ttgattcatt gaattatcct gaaaattgcg ggtctgtcag gtggaaacct
     8521 gtgaccagaa tagaagtgag ttagtaacac ctacccaatc agcacgttaa ttttggcttt
     8581 aatgagttgt aattcctctg gggcaactgt tcctttcttc gttgctcctc ttgcccgcca
     8641 ggcgatactt tttacctgat cagctaacgc tacgccatca cgttcctgac cggatagaac
     8701 aacttcgaac ggatatcctt ttgattgcgt tgtacaagga acacacagac acatacctgt
     8761 tttgttgttg tacatgaacg gactcaggac aacagccgga cgatgtccgg cttgctcgct
     8821 accttttgtc gggtcaaaat caacccaaat cagatcgccc gtatcgggta cgtatcggct
     8881 taccattacc agacttcctt atctttcggc tccccccagt cgatattctc gtggaggttt
     8941 tccggcgtga tgtcgttgac cagttcagca agcgtaaata cgggctcttt acgcactggc
     9001 tcaataatta atttgccatc caccaggtca atcttcactt catcatcaat attcagattg
     9061 agcgcctgca ttaacgtagc cgggatccgc accgccggtg aatttcccca acgctttacg
     9121 ctactgtgga tcataaccct ttcctcaaac cgcgatcata tgtagataca gtatatatca
     9181 atctacattg tagatacgag caagtttcgg cctaactccc gtgcaaccga cgcgcgtcga
     9241 taacatccgg tacctggttg agtttaccca gcacgcgacc cagcacttgc aggttgtaaa
     9301 tctcaatggt catgtcgatg gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc
     9361 caagtacgtt caccttctcg ttggcgagaa tggtcgtgat atcacgtaac aacccactac
     9421 gatcattagc caccacgcgg accaccagcg aatatccggc ggagtagctc tcgccccata
     9481 ccgcgtcaac aatgcgttct ggcgcatggg agcgcagttc cgccaattgt tcgcaatcgg
     9541 cgcggtgtac tgaaataccg cgcccctggg taatgaagcc gacaatctca tctccaggaa
     9601 tcggctggca gcagcgcgcg atgtggtgca tcaggttacc gaccccttct accaccacac
     9661 gaccgttatc tttactgcgg ttttgcggcg tgtagctttt ttgctgaagc tgtttcagcg
     9721 cggcggcgtc ctgctcttcg gcactcggct tattaaattg cgattgcagg aagttcacca
     9781 tctgattgag acggatatcc ccgccaccaa tcgccgccag caactcgtcg acatcattga
     9841 agttgtaacg cggcagcaga tgtttttctg cttctttcag gctgatcccc agatgttcca
     9901 gctcgtcgtc aaggatttgc cgcccagcca gaatgttttt gtcacggtcc tgtttacgga
     9961 accaggcgtg aattttcgaa cgcccacggc tggttgtgac gtaaccgagg tttgggttta
    10021 accagtcacg gctggggttc ggctgtttct gggtgataat ttcaatctgg tcgcccatct
    10081 gcagctggta ggtgaacggc acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc
    10141 cgacatcact gtggatgtgg taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa
    10201 cgacatcacc tttcggcgta aagacgtaca ctcggtcgtc aaagacctga ctacgtactt
    10261 cgtcgagcat ttcgccggaa tcagccatct cttcctgcca cgcaatcagt ttacgcagcc
    10321 aggcaatccg gtcttcatgt cccgaacgtg cgccgccagc agccgcgccc tctttatatt
    10381 tccagtgcgc agcaacaccc aactctgcat cttcatgcat ctgtttggtg cggatttgga
    10441 tctcaacggt tttgccaccc ggccccagaa ccacggtatg aatggactga taaccgtttg
    10501 gtttcgggtt agcgacgtaa tcgtcaaact catccggcag gtggcgatag tgagtgtgca
    10561 ctatccccag tgcggcataa caatcctgta aacgctcggc gacaatacgt accgcacgca
    10621 catcaaacag ctcatcgaag gcgaggttct ttttctgcat tttgcgccag atactgtaga
    10681 tgtgtttcgg acggccatac acttcagctt taacgccttc agctttcatc tcagcgcgca
    10741 gatggccgac aaactcttcg atatagtgtt cgcggtcgag acggcgttca tgcagcagtt
    10801 ttgcgatgcg tttgtattcg gtcgggtgga gataacggaa gcagtaatct tccagctccc
    10861 atttcagttg cccaatccca agacggttcg ccaacggcgc gtagatattg gtgcactctt
    10921 ttgccgccag tacgcgttca tcttccggcg catcttttac ttcgcgcagg tgagcaatac
    10981 gctccgccag tttgatgacc acgcagcgaa aatcatcgac catcgccaat aacatccggc
    11041 gaacgttatc gacctgttcg gaggaaacag aatcagtgtg cgtcgctttc agctggcgga
    11101 tcgccgccat atcacgcacg ccgtgaataa ggttaacgac cgacttaccg acgctctcac
    11161 gcagcacatc ttcgctgact acgttggcat cagccagagg gaacagcagc gccgcccgca
    11221 gcgtgtcaat gtccatactt aatgtcgaga ggatctccac catctcaaca ccacgccaca
    11281 ataacagact ggcatccgga tgcccctgcg tctgttgcag acaatacgcc caggtttcgg
    11341 ctaagcactc acacgacttc tggctggtaa tacccagact tgcgatccat ttttccggat
    11401 caaattcacc agccttattg atatgtgcac ttcttaccgc aaccatcgtc ctctccttta
    11461 gggaccagac ctgccgaaat cggcaaatcg taactattta acgcgcgaga aaagtaccat
    11521 cgattccaga tgtcccgtgt gtgggaacat atccagcatc gccagtcgcg caatggtata
    11581 tcctgctttt aataacgctt cgctatcccg agccagcgtt gcagggttac aggatacata
    11641 aactatacga ataggttcca gttttataat ttgctgcata acacctgcgg cacctgctcg
    11701 cgccgggtcc aacaacactt tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc
    11761 ttcttcaaga ttttcgtgat aaaacgtcac attctgtaag ccattaagac gcgcattctg
    11821 ctggcctttt tccaccagcg ccggaacacc ttctacaccc accacactgg cagcttgtgt
    11881 cgccaatggc agtgtaaagt tgcccatacc gcagaacaga tccagtacgc aatcttcagg
    11941 tgccacatcc agccattcca acgcacgcgc taccattttc tggttcacac ccgcattgac
    12001 ctgaataaaa tcgcgcgggc taaaagttaa gcgcaaccca tttgagtcat accagggcat
    12061 ctcaccagag acggtttcga gtatctcact atcgggggcg agatacagat ccaggccttc
    12121 agaatgcgaa aagcattcca gtttttcgcg atctgccgaa cttagcggtg cggtatggcg
    12181 caaaatcatt agcgtgccgc tggttgcctg taccagttca acatgaccaa gatggcgcat
    12241 agcttgtaag ctgcccaggc atgccctgac tttgggcagc aatgcttcaa gttggggcac
    12301 taaaatgggg cattgtttca cgtcgacaat gtcactggag cccgctttgc gaaaccccat
    12361 ctgaagttgc tgtgttttcg gtaagtagtt cagacttaaa cgcgcgcgac ggcgatagcc
    12421 ccagggaaca tcggcgatga cttcagagac ttcgtgtttc attaatcggg cgagtgccgc
    12481 acttttgctt cgctgctgta aatccacact ggcgtgttgt tgctgacagc cgccgcatac
    12541 gccaaaatga ggacagcgtg gcgtttcgcg ttccgggcta tcgcttaacc gacgtacgac
    12601 tttagcgcgg gcatactgtt ttttatcttc agtaacagta acttccgcgt tttcctgcgg
    12661 caataatccg gggataaaca acgttttgcc gttatgtcgc gccacgccct gaccaaaaga
    12721 gtcgaggtcg ttgactgaaa cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga
    12781 gtagaattgc gccattggcg agactttctc aatttaacag tgtgacctta attgtcccat
    12841 aacggaactc catgaccaac tacagcctgc gcgcacgcat gatgattctg atcctggcac
    12901 cgaccgtcct tattggttta ttgctgagta tctttttcgt cgtgcatcgc tataacgact
    12961 tgcagcgtca actggaagat gccggtgcca gcattattga gccgcttgca gtttctactg
    13021 aatatggcat gagtctgcaa aatcgcgaat ctatcggtca gttaataagc gtactgcatc
    13081 gtcgccattc tgatattgtt cgcgcgattt cggtttatga tgaaaataac cgactctttg
    13141 tcacctccaa ttttcatctt gatccctcat caatgcagct cggcagcaac gtgccgtttc
    13201 ctcgccagct cactgtcact cgtgacggcg atataatgat cctccgcacg ccgattattt
    13261 ctgaaagtta ctcccccgac gaatcgccca gtagcgatgc caaaaatagt caaaatatgc
    13321 tgggatatat cgcgctggag ctggatctta aatcggttcg cttgcagcaa tataaagaga
    13381 tctttatttc cagcgtgatg atgctgtttt gtatcggtat tgcgcttatt tttggctggc
    13441 gcttaatgcg cgatgtaacc ggtccgattc gcaacatggt gaataccgtc gaccgcatcc
    13501 gtcgcgggca actcgacagc cgggtggaag gatttatgct cggcgagctg gatatgctga
    13561 aaaacggtat caactcgatg gcaatgtcgc tggcggctta tcacgaagag atgcaacaca
    13621 atatcgacca ggcgacgtcc gatctgcgtg agacgctgga gcaaatggag attcagaacg
    13681 ttgagttgga tctggcgaaa aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc
    13741 tggcaaatat gtcacacgag ctgcgtacac cactgaatgg tgttattggc tttacccgcc
    13801 tgacgctgaa aacagaatta acaccaacgc agcgcgatca cctgaatacg attgaacgtt
    13861 cggcaaataa tttgctggca attattaatg atgttctcga cttctcgaaa ctggaagccg
    13921 gtaagctgat tctggaaagt attccattcc cactacgcag cacgctggat gaagtcgtta
    13981 ccctgctggc acattcttct cacgataaag ggctggaact gacgctcaat attaaaagcg
    14041 acgtgcctga taacgtgatc ggcgacccac tacgattaca gcaaatcatc actaacctgg
    14101 tagggaatgc aattaaattc accgagaatg gcaacatcga tattctggta gaaaaacgtg
    14161 cgctgagtaa taccaaagtg cagattgaag tgcagattcg ggataccggc attggtattc
    14221 ctgaacgcga tcaatcgcgc ttattccagg ccttcagaca ggctgatgcc agtatttccc
    14281 gccgtcatgg tggcaccggg ctggggctgg tgattacaca aaaactggtt aatgaaatgg
    14341 gcggcgatat ttcgttccat agccagccga atcgcggttc aactttctgg ttccacatta
    14401 atctcgatct gaacccgaac attattatcg aagggccatc cacccagtgc ctcgctggta
    14461 agcgcctggc ctatgtcgaa ccaaactccg cagcagcaca atgcacgctg gatattttaa
    14521 gtgaaacgcc gctggaagtg gtttatagcc caacgttctc cgcgctgcct cccgcgcatt
    14581 acgacatgat gttattaggc atagcggtga ccttccgcga gccgctaaca atgcaacatg
    14641 agcgattagc gaaagcagta tcgatgaccg atttcctgat gctggcactt ccttgccatg
    14701 cacaagtcaa tgctgaaaaa cttaagcaag atggtatcgg cgcgtgtctg ctgaaaccat
    14761 taacacctac gcgcctgttg cctgccctga cggaattttg tcatcacaaa caaaacacgc
    14821 ttttgcctgt aaccgatgaa agtaagctgg caatgacagt catggcggtt gatgacaacc
    14881 ccgctaacct gaaacttatc ggcgcattgc tggaagatat ggtgcaacat gtggaacttt
    14941 gcgatagcgg gcatcaggcg gttgaacggg cgaaacagat gccgttcgat ttaatcttaa
    15001 tggatattca aatgccgaac atggacggca ttcgggcatg tgagctcatc caccagctcc
    15061 cgcatcagca gcaaacgccg gttatcgcgg taacggcgca tgcgatggcc gggcaaaaag
    15121 agaaactgct tggcgcgggg atgagtgatt atctggccaa accgatcgaa gaagagcgat
    15181 tgcataattt gctgttgcgc tacaagcctg gcagcggtat ttcctctcgc gtcgtgacgc
    15241 ccgaagtcaa cgaaattgtg gtcaacccga atgcgaccct cgactggcaa ctggcactac
    15301 gccaggcagc aggaaaaacc gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc
    15361 tgcctgaagt tcgcaacaaa gttgaggaac agctggttgg agaaaacccg gaaggcctgg
    15421 ttgatttgat tcataaactg catggcagtt gcggctatag cggtgtgccg cgtatgaaga
    15481 atatctgcca actgatcgaa caacagctac gtagcggtac taaagaagaa gatttggagc
    15541 cggagctgct ggaactgtta gacgagatgg ataatgtagc gcgcgaagcc agcaaaattc
    15601 tcgggtaacg gacgtcttgt cgagtcaaaa taggttccgt ccaatgccaa tgctgggagc
    15661 agcacagaca gtcccctcgc ccctccgggg agagggttag ggtgagggga acaggccaac
    15721 gcaagtgcga atgattaccc tcaccccggt cctctccttg aaagggcgag gggaaaaccg
    15781 tccccgtaag ccacttcgtc agtaatctca aaccggctcc tgaaagagcc ggttttggtt
    15841 ttctgtctta acgcaccatg cacggacgct tgttatcgaa cgtccagccg gggatcaggt
    15901 actgcattcc catcgcatcg tcacgcgcgc caagcccgtg tttctgatac agctcgtgcg
    15961 ctttcatcac ttgatccata tcgatttcta cacccagccc cggtttttct ggcacctgaa
    16021 ccagaccgcc tttgatctct aacggttctt tggtcaggcg ctgattacct tcctgccaga
    16081 tccagtgcgt atcaatcgcg gtgattttac ccggtgcagc agcggctaca tgggtaaaca
    16141 tcgccaggga aatatcgaag tggttgttag agtgtgaccc ccaggtcagg ccaaattcat
    16201 ggcacatttg cgccacacgt accgaacctt gcatcgtcca gaaatgcgga tccgccagcg
    16261 ggatatcaac ggattgcagg gagagcgtat ggcccatctg ccgccagtcg gtagcgatca
    16321 tattggttgc ggtcggcagg ccagtcgcgc gacggaactc tgccatcact tcacgcccgg
    16381 agaaaccttg ctccgcacca cacggatctt ctgcataagc cagcgaacct ttcaggtatg
    16441 taccaatttt aatcgcttcg ttcagcgacc aggcaccgtt aggatcgagg gtaatacgcg
    16501 cctgtgggaa gcgttgcgcc agtgccacaa tagattcggc ttcttcttcc ccggccagta
    16561 caccgccttt cagtttgaaa tcgttgaagc catatttttc atacgccgct tccgccaggc
    16621 gcaccaccgc atccggcgtc atcgcttctt catgacgcag gcgataccag tcgcatgagt
    16681 catccggctg gctttgatac ggcagcggtg tggctttgcg attaccgacg aagaacagat
    16741 aaccgagcat ttcgacttcg ctacgctgtt gaccatcgcc cagcagcgat gccacgttta
    16801 cccccagatg ctgccccaac agatccagca ttgccgcttc tatcccggta actacatgaa
    16861 tagtggtacg caaatcaaat gtctgcaaac cgcgcccacc ggcatcacga tcggcaaaag
    16921 tattacgcac cagcgtcaga acgtttttgt attcacccag cgttttaccc accaccagcg
    16981 gaatcgcatc ttccagcgtt ttacggattt tctcgccgcc gggaatttcc cctacgccag
    17041 tgtgaccgga attatctttg ataatcacaa tattacgcgt gaagaacggt gcatgtgcac
    17101 cactcagatt catcagcata ctgtcatgac ccgccaccgg gatgacctgc atttcagtaa
    17161 caacaggcgt cgtaaattga gaactcatat ttatgtcctt tttcagaatc aatgacggcc
    17221 gaaaacggga cgtttacggt caaaggtcca gccggggatc aggtactgca tcgggcctgc
    17281 gtcgttacgc gcaccgccag gcagacgttt ataggcctca tgtgcctttt gtacctgttc
    17341 ccagtccagt tccacgccca gaccgggcgc atcaggaacg gcaatttttc cgtttttaat
    17401 ctccagcgga tttttggtca gacgacaatc gccctcctgc caaatccagt gggtatcgat
    17461 agcggtagga ttacccggtg ccgccgcgcc cacatgggta aacatcgcca gagagatatc
    17521 aaagtggtta ttagaatggc agccccaggt cagcccccag tcgtcgcaaa gttgcgccac
    17581 gcggactgca ccggaaagcg tccagaagtg cggatcggca agtggaatat ctaccgcatt
    17641 gagcatcacc gcatgaccca tttcgcgcca gttggtggcg atcatgttgg tcgcgacggg
    17701 caagccggtc gcccgtcgaa actccgccat cacttcacga ccggagaaac cctgctcagc
    17761 gccgcacggg tcttccgcat aggtaaggac atcattcagc cctttgcaca gagaaatggc
    17821 ttcatcaagc agccaggcac cattgggatc aacggtaatc cgcgcatcag ggaagcgttt
    17881 cttcaatgca cgaacagtat cgatttcttg ctcgccaggt aacacgccac ccttaagttt
    17941 gaaatcttta aagccatagc gatcctgtga ggcttccgcc agacgcacaa cggcttcgct
    18001 gttcatcgct ttctgatgcc gcaactgata ccactcatgg ttgcccggcg tattttccag
    18061 ataaggaaga tcggttttgg tccgatcacc gatataaaac agataaccga gaacggtaat
    18121 agcatcacgt tgcttgcctg gccctaacag ttcgcagacc ggaacattca gcgccgccac
    18181 ggcattaacg cgcaattcaa aagtccaggc acctttgccg aaggtatcaa aatcggctgc
    18241 ctgattacct ttatgcacct gctgaaccac tttattcagg cgcgcaactt cctggcccag
    18301 caccatcgga atagccttga ccagtgtttg ataaatcacc tctccgcccg gcgcttcacc
    18361 aatgccggta tgcccggcat tatcggtgag taccacaata ttgcgggtga aatatgcgtt
    18421 atgtgcacca ccaatattaa gcaacatgct gtcatgtccg gcgaccggaa tgactttcat
    18481 atcagtaata acaggactgg attgtgtcgt catcttattg ccccgcgaca ggtttcagct
    18541 caatacgctt gatatcgccc accagcacca ggtagctgag taccgcgatt aaggcatgaa
    18601 caccaacata aatcagcgcc ccattaaagg agccagtcgt gccaacgata taaccaattg
    18661 cgattggcgt gacgatgcca gaaatgttac caaacatgtt aaacaggcca ccggaaagac
    18721 cgctgatctc ttttggcgca gtatcagcca ttacagccca acccagcgca ccgatcccct
    18781 taccgaagaa ggccagcgcc ataaagccga tgatcatcca ctcaacgttg acgtagttgc
    18841 agaacaccat caccatcgac aacaacatgc ccatcacgat cggtgtttta cgcgcaatgt
    18901 tcagtgaacc cgtgcgacgc atcagccagt cggaaataat cccacccagc acaccgccga
    18961 taaaaccgca aaccgccgga acggaagcca caaagcccgc tttcagaatc gacatcccgc
    19021 gcgcctgcac cagataaacc gggaaccagg taataaaaaa gtaagtcagg gcgttgatac
    19081 agtactgacc gatataaacg ccgatcatca tccgtgaccc gagcagctgt ttgatctgcc
    19141 cccacttcac gctaaacggc actttaactt tggtgttttg ctgatccata ttgatcagcg
    19201 cgccacccgc ggcgatgtac tccagctctt tctgatttac gcccggatgt tgatttggct
    19261 cgtggatgac tttcaaccag ataaagctga tgacaatgcc cagaccgccc ataaagaaaa
    19321 agacgtgtga ccagcccact tcatgcgtca gccagcccat aatcggcgcg aagataaccg
    19381 ttgcgaagta ttgagcggag ttaaaaatcg acaccgccgt tcccctttcc tgcgccggaa
    19441 accaggccgc aacaatgcga ctgttgccgg ggaaagaggg cgcttcagca agcccgacca
    19501 ggaagcgcag cgtaaacagg gcaacgataa tgccgaatcc actaaagata tcgacgaagc
    19561 cttgcagcaa ggtaaacatc gaccagataa agatcgacca gaagtagacg cgttttgaac
    19621 caaaacgatc cagcaaccag ccaccaggga tctgcccgat aacatacgcc catgagaagg
    19681 cagagaacac atagcccatt cccacgggat caaggccgat atctttggcc atttccgaac
    19741 cggcaataga aagcgtagcg cggtcgccgt agttgaagga tgtgacgata aacaacatca
    19801 ccactatcca gtaacgagca ttcgtgcgtt tttccacact gctcgcagcc tgacttaaag
    19861 aactcattgt tgcactcctg aaaattcgcg ttagccacgc tcactctgga ctgcgacatc
    19921 gccaggaaat cagaggtgac gtagggtgtt tttgccgttt ttataggtcg ttcgccgaat
    19981 acggcgcgtg tttatatctg gcaatagcag tataaaaagc gcgccatagc ggctcaccgt
    20041 gcaacaacac aacattaatg cgttcaatga ggcccgattt tggcattagc cctggacggt
    20101 ggaatccact tcacggaaat gaaaacaaga acaagaaagg aagggttaaa acgaagaaat
    20161 aaagaagagt atgaaatgga tcgcttgact ccaggcaaac gccagtaaaa atccgcgcta
    20221 tgaagcagtt tttactggca tttgcctgaa aagattcgat tcagcaccgc taaaacggca
    20281 tttaccgctc gctgaacata tcaggacaac atcgtgcccc aatgttcaac ccacggattt
    20341 gattccgttt ccggttccgg gttctcactg gcgtcaatca acagcatttc gccaacccgc
    20401 tgagcgctct gttcctgcaa caaggcatcg aactgtttgc cgccattgca gaaattcaca
    20461 taactactgt cgccgagcgc aatcacgcca taacgcagat tcggctggaa gcccagacta
    20521 tctttgattc cctgaaagag cggcacaatg ctatcaggaa ggtcgccctg cccggtcgtg
    20581 gacgtaacca ccagaacata cttatcctga taaggcagcc agtcgcttaa ttcaggatct
    20641 tcaaataccg ttgctttgtg gccctgcgcg gtcagaatcg cttccgcttc ttcggccact
    20701 aacagtgaat tcccgtacat ggtgccgaca aaaataccaa tttccgccat agcgttttct
    20761 ccctggatta ggaacttatc tctccatcct gacccgatgg tgcactgaac tcaacccttt
    20821 cattttcagg aagcagaccg cgccagccaa attgtgataa tgcctgcatc caggtgtcgt
    20881 ccaggcccgc gtgaatagcc agtagctcgc cagtaaaagg atgcgtcaat gacaactgac
    20941 tggcatgcag cattaaccgc tgaaggccaa aatgctcagc accgctgcga ttctggcgta
    21001 aatcaccatg tttgctgtcg ccaataatcg ggtgccgcaa atgggcaaga tggcgacgga
    21061 gctggtgctt gcgtccggtt ttcggctcca gttccaccag gccgtagcgc gtggtcgggt
    21121 aacgtccggt cgctaccggc atttctacgg tcgccagacc gcgataatgc gtcactgctg
    21181 gctgcgggcc tttatcttcg cgggcaaatt tatcagcgat tttgtccagt tcttccacca
    21241 gtggataatc cagcaccgct tcttccatca accagccacg cacaatcgca tggtaacgtt
    21301 tctggatttg gtgctgttca aactgttgtg ccagcagccg tccggcttcg ctggataatc
    21361 ccatcaacaa cacaccagaa gttggtcggt ccagacgatg agcagtaaaa acatgctggc
    21421 ctatctggtc acgcacggtt tgcatgacca ctactttctc gtcgcgatcc agccagctgc
    21481 ggtgaaccag ccagccggag ggtttattta ccgcaaccag ccattcatcc tgatagagta
    21541 tttccagcat tagctcgcat catccgcaaa aagagcatcc agtttctcca gctcagccag
    21601 aataagcgcg cgttgcggat gatcagtcgc cagcgccatt tcataatagg gcgcaacagc
    21661 aaaagcgccc ggtaacggct ggttgttatc taacaaatcg tgcattcgcg ggatcagcac
    21721 ccactgcaac cactccagtg gttccatggt gtccataaag aacggctggg tactcgcaaa
    21781 ttgatgcggc aggggttcat cgtttcgcca gtgctgatgt tcacgcagta acgcttcaag
    21841 cgcctgcaac tggagacgaa cgcggtcatg agtggtcata aagcaacctc aataagaaaa
    21901 actgccgcga agaatagcag gaataaaaaa agggagcact gtattcacag tgctcccggt
    21961 tcgtttcgca gcattccggc tacttttatt gctccctgct catccttgac aacttttcct
    22021 ctggccttgc ggccaatcgt tcatcctgaa ctattgcttc ctgctcacac caccccgatg
    22081 tgatacttca tcctgaagtg ttcctggcct tcctgaccca ccgaatcatc ctgaccggtt
    22141 ctcattctcc atcctggagg tgtcctttaa cgcgtcctgc gtcatcctct tcgcttcatc
    22201 cagaagcctt tccctgaaac accatcctgg tgtgtccttc agaagtgtca tcatcctgat
    22261 gttcacttcg ttgtctgact ccctgtcgac gaagatagaa tcgtcttttt ctcgaagtct
    22321 tacaaggcgc ttacagacaa tgccttaagg aaaatttcat atgaaagtta agaattaaca
    22381 agcaacatgt tgtttttaaa tgagaataac gattcttatc tttttaagta ttcgcttatt
    22441 tcctggcgat ctcctacaga gtctgtaaga gatctctcac aaccagatgg ggtatttgga
    22501 ttacagaaga ggcttaagtt gattaaggaa ttccgcaaga tttgaggcta aatgcgtccg
    22561 tttacgtgtt cccagcgtct ctttgcacac ttcaccggag agattacaca ccgagatgac
    22621 ctctaactct tcttccagcg tcgcgataaa tagcgtgggc ggcagtttta gccttttctg
    22681 agtcaccaga tgaccaatca gattttcctg cacccgacgg aagtcgtctt cactccagat
    22741 ttgcagcaat gtcagtttga tatcgccaaa ctgcgcgtgc atatccccgg caaactgagt
    22801 cgtataaaag gtatgaatcg tgggttgtat cacaatatca aaagcgcgtt caaccgcgtt
    22861 tacattttgt tccccggtaa acggctgggg ttgccagtat acggcatctt cggtagttga
    22921 aataatgcac ggtgaaggaa cgccgtacag ttcctcactt aacggccagc ttttatgctc
    22981 ttcatgccac gcatcgcagt agcgtgcagt aaaatctttc agggctcgtg cggtcaaatc
    23041 gtccactaat ttctctcttc acttaaacca gatacacttg tcgtttagtt tatctggttt
    23101 atgacggtga aacatgtctt cttatgcaaa ccatcaggca cttgcgggcc tgacacttgg
    23161 aaaatcaacc gattaccggg atacctatga cgccagccta ctgcaaggcg ttccacgcag
    23221 cctgaatcgc gacccgctgg gtctgaaagc ggataacctg ccttttcacg gtacggatat
    23281 ctggacgctg tatgaacttt cctggctgaa tgcgaaaggt ttgccgcagg tcgctatcgg
    23341 tcatgttgaa cttgattaca ccagcgtaaa tctgattgag tcgaagagtt ttaagctcta
    23401 tctcaacagt tttaaccaga cgcgttttaa taactgggat gaggtgcgcc agacgctgga
    23461 gcgcgactta agcacttgcg cccagggtaa ggttagcgtg gcgttatatc gtcttgatga
    23521 actggaaggc cagccgatag gtcatttcaa tggcacctgc attgatgacc aggatatcac
    23581 tatcgataac tatgaattca ctactgacta tctggagaat gccaccagcg gtgaaaaagt
    23641 agtggaagag acgcttgtca gccacctgct gaaatcaaac tgcctgatca cccatcaacc
    23701 agattggggt tcgatccaaa ttcagtatcg tggacgccaa attgacagag aaaaactgct
    23761 gcgttacctg gtctctttcc gtcatcacaa cgagttccac gaacagtgcg tggaacgcat
    23821 ctttaatgac ctgttacgct tctgccagcc agaaaaattg agcgtttacg cacgttatac
    23881 ccgtcgtggc ggtctggaca ttaacccgtg gcgcagtaat aacgattttg tcccatcgac
    23941 cacaagactg gttcggcaat aaattttttc tcaattttgc gtgctggatt cacgcagaag
    24001 gttgtgaaag gtcagcaggc agggctattg taatcaaagg gaatgacgat attcgtccca
    24061 taaggagttt ttcttgatta cacatattag cccgcttggc tccatggata tgttgtcgca
    24121 gctggaagtg gatatgctta aacgcaccgc cagcagcgac ctctatcaac tgtttcgcaa
    24181 ctgttcactt gccgtactga actccggtag tttgaccgat aacagcaaag aattgctgtc
    24241 tcgttttgaa aatttcgata ttaacgtcct gcgccgtgaa cgcggcgtaa agctggaact
    24301 gataaatccc ccggaagagg cttttgtcga tgggcgaatt attcgcgctt tgcaggccaa
    24361 cttgttcgcg gttctgcgtg acattctctt cgtttatggg caaattcata acaccgttcg
    24421 ttttcccaac ctgaatctcg acaactccgt ccacatcact aacctggtct tttccatctt
    24481 gcgtaacgct cgcgcgctgc atgtcggtga agcgccaaat atggtggtct gctggggcgg
    24541 tcactcaatt aacgaaaacg agtatttgta tgcccgtcgc gtcgggaacc agctgggcct
    24601 gcgtgagctg aatatctgca ccggctgtgg tccgggagcg atggaagcgc cgatgaaagg
    24661 tgctgcggtc ggacacgcgc agcagcgtta caaagacagt cgctttattg gtatgacaga
    24721 gccgtcgatt atcgccgctg aaccgcctaa cccgctggtc aacgaattga tcatcatgcc
    24781 agatatcgaa aaacgtctgg aagcgtttgt ccgtatcgct cacggtatca ttatcttccc
    24841 tggcggtgtg ggtacagcag aagagttgct gtatttgctg ggaattttaa tgaacccggc
    24901 caacaaagat caggttttac cattgatcct caccggcccg aaagagagcg ccgactactt
    24961 ccgcgtactg gacgagtttg tcgtgcatac gctgggtgaa aacgcgcgcc gccattaccg
    25021 catcatcatt gatgacgccg ctgaagtcgc tcgtcagatg aaaaaatcga tgccgctggt
    25081 gaaagaaaat cgccgcgaca ctggtgacgc ttacagcttt aactggtcaa tgcgcattgc
    25141 gccagatttg caaatgccgt ttgagccgtc tcacgagaat atggctaatc tgaagcttta
    25201 cccggatcaa cccgttgaag tgctggctgc cgacctgcgc cgtgcgttct ccggtattgt
    25261 ggcgggtaat gtaaaagaag tcggtattcg cgccattgaa gagtttggtc cttacaaaat
    25321 caacggcgat aaagagatta tgcgtcgtat ggacgacctg ctacagggtt ttgttgccca
    25381 gcatcgtatg aagttgccag gctcagccta catcccttgc tacgaaatct gcacgtaatc
    25441 tcccctcccc cggtgacgtt tgttgccggg ttttctcgtt tttggtcgct tactcatcaa
    25501 ctcatttcat ttgttatatg aatgtttctt accaccctca cgcgacaaat atcaccacag
    25561 ttaatatgtc atacaattta tgttgaaacg caaacgtttc cctattttca taaaccacat
    25621 tttatcgcca tgaaaaagaa attatcgcca tgattaacta aaagtattga ttttttcag
//
LOCUS       DAZGJT010000069        24639 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZGJT010000069 DAZGJT010000000
VERSION     DAZGJT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24639
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.069"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..361)
                     /gene="yehE"
                     /locus_tag="ACHKFK_003052"
     CDS             complement(80..361)
                     /gene="yehE"
                     /locus_tag="ACHKFK_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO9053336.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVIAADSDSKRRIVLNRYD"
     gene            complement(624..1733)
                     /gene="apbC"
                     /locus_tag="ACHKFK_003053"
     CDS             complement(624..1733)
                     /gene="apbC"
                     /locus_tag="ACHKFK_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO9053337.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1865..3898
                     /gene="metG"
                     /locus_tag="ACHKFK_003054"
     CDS             1865..3898
                     /gene="metG"
                     /locus_tag="ACHKFK_003054"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO9053338.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAIREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4039..7827
                     /locus_tag="ACHKFK_003055"
                     /pseudo
     CDS             4039..7827
                     /locus_tag="ACHKFK_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            7837..11469
                     /gene="yehI"
                     /locus_tag="ACHKFK_003056"
     CDS             7837..11469
                     /gene="yehI"
                     /locus_tag="ACHKFK_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO9053339.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSITARTMAALACIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFIVRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCVDKLIAALPGITKVRRPFIALNLPEKPEIANELVSL
                     ECSQTHFRSKEWLKVVADDPKAVKELARYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAMYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKAPPARPGYPIIEDKKLPAQQKARDEYWHTLLQTLMASQPQLAEE
                     VMPCLSTQAQAVVNGYLSASPKLAFESTHSNDLPEILVSPPWRGKKKTALLRLDLVPL
                     ELAPKARWQPGERERLAATESARYFSTGSFTERMERKSGRVVLQELGFGDDVWLFRNY
                     ILPGKLDAARKSLVGQWHYSPRRVEEINNGWHSTEAKSAEQALRSGDVEALINTWEND
                     SYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDTLPGLL
                     LAFSHHPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTATALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWPALEQLLKLGPIEIYPA
                     RIPKAPDFWHPAMWSRPRLIKNNQPVTDDALEIIGEMLRFTQGGRFYGGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRSLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALIFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLETALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNEQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESASNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGAYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11530..11846
                     /gene="yehK"
                     /locus_tag="ACHKFK_003057"
                     /pseudo
     CDS             11530..11846
                     /gene="yehK"
                     /locus_tag="ACHKFK_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
     gene            12551..13639
                     /gene="yehL"
                     /locus_tag="ACHKFK_003058"
     CDS             12551..13639
                     /gene="yehL"
                     /locus_tag="ACHKFK_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFO9053340.1"
                     /translation="MSPQNNHLQRPPATVLYAVELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            13650..15929
                     /gene="yehM"
                     /locus_tag="ACHKFK_003059"
     CDS             13650..15929
                     /gene="yehM"
                     /locus_tag="ACHKFK_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFO9053341.1"
                     /translation="MSEPLIVGIRHHSPACARLVKLLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALARYFA
                     QLRGDYPGDALNRQREAFMARWITWAMQQNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQCGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVINPDTDPILITLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTGISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGVVRQQGSTLTLAGNGEECWKLTRPLSQHAAL
                     IEAACFGATLQEAARNKLEADMLDAGGIGCITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHTHLHSWQALCHILRDLHSGVNLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPTQSGEALHGLLALARHQLACQPVFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLVQLPVSALQMPLHCPPQDIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            15922..17058
                     /gene="yehP"
                     /locus_tag="ACHKFK_003060"
     CDS             15922..17058
                     /gene="yehP"
                     /locus_tag="ACHKFK_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO9053342.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERYGGLGRSNLTTPEWINSIHTLFPQRVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVHQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFISLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRHSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSSATPCYDHDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            17055..19058
                     /gene="yehQ"
                     /locus_tag="ACHKFK_003061"
     CDS             17055..19058
                     /gene="yehQ"
                     /locus_tag="ACHKFK_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO9053343.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCTCGASGMCRHRVMLVLSYQRLCATAQPTEKKE
                     EEWDPAIWLKELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRS
                     SIRFARCDCIEGTLCEHVVLAVQAFVEAKTQQAEFTHLIWQMRSEHATSSDAPFASEE
                     GKTCRQYVQQLSQALWLGGISQPPIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVD
                     AFHARASHYHAGECLRQLAALNSRLNCVQEMARRDSIGEVPPMPWRTVVGAGIAGEAK
                     LDHLRLVSLGMRCWQDIEQYGLRIWFTDPDTGSILHLSRSWQRSEQENSPAATRRLFS
                     FQAGALAGGQIVSQAAKRSADCELLFATRNRLSGVVPLSPDAWLMLSAPLRQPGIVAL
                     REYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNVLTLS
                     LPASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAE
                     TAPVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAIGQAELLANDLTA
                     VGFYRLAHVLGQFRNTESEARVEAMNNGVLPCEQLFPMLQQQG"
     gene            19183..19644
                     /gene="yehR"
                     /locus_tag="ACHKFK_003062"
     CDS             19183..19644
                     /gene="yehR"
                     /locus_tag="ACHKFK_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFO9053344.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAARTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQAISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(19685..20155)
                     /gene="yehS"
                     /locus_tag="ACHKFK_003063"
     CDS             complement(19685..20155)
                     /gene="yehS"
                     /locus_tag="ACHKFK_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO9053345.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(20202..20921)
                     /gene="btsR"
                     /locus_tag="ACHKFK_003064"
                     /gene_synonym="yehT"
     CDS             complement(20202..20921)
                     /gene="btsR"
                     /locus_tag="ACHKFK_003064"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO9053346.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(20918..22603)
                     /gene="btsS"
                     /locus_tag="ACHKFK_003065"
     CDS             complement(20918..22603)
                     /gene="btsS"
                     /locus_tag="ACHKFK_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO9053347.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            22825..22890
                     /locus_tag="ACHKFK_003066"
                     /pseudo
     CDS             22825..22890
                     /locus_tag="ACHKFK_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23118..23366
                     /locus_tag="ACHKFK_003067"
     CDS             23118..23366
                     /locus_tag="ACHKFK_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9053348.1"
                     /translation="MRVEICIAKEKISKMPKGSVSALQQEMLRRVSKRYDDVDVIVRA
                     ANNDSLTILRAVDKESAHEFVQETLQEAWETADDWFIR"
     gene            complement(23403..23576)
                     /locus_tag="ACHKFK_003068"
     CDS             complement(23403..23576)
                     /locus_tag="ACHKFK_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053349.1"
                     /translation="MLPDLQRLQLIQGQMMNKDKCRMYGRKRYTAGAMCFMLFVWLGG
                     VVTLCAVAAIIIQ"
     gene            complement(23581..24054)
                     /locus_tag="ACHKFK_003069"
     CDS             complement(23581..24054)
                     /locus_tag="ACHKFK_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053350.1"
                     /translation="MKVILATRNRYLEYGLQALLKEHSVIVAREFFLPENRRYIPDFD
                     ESWLIICDVLLGRLMRCMFQGRHFLQLGAELLRDGEQISDAIHNGVWTYNSAARPLTM
                     SEMVVMFGYVYRQSRPCRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVKHLACAE"
     gene            complement(24144..>24639)
                     /locus_tag="ACHKFK_003070"
     CDS             complement(24144..>24639)
                     /locus_tag="ACHKFK_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA C-terminal domain-containing protein"
                     /protein_id="HFO9053351.1"
                     /translation="LDENQGSGERYSVKQTVADIKADTTVYQNKDGSYTLDQSAPGNV
                     RVNDAVVSLDNRTRSNTQAIQNHSRQLQEHNARLNSQQRQIRENHEEMKRAAAQSAAL
                     SGLFQPYSVGKFNATAALGGYSDKQAVAVGVGYRFNEQTAAKAGIAASDGDVSYNVGV
                     NFEF"
BASE COUNT         6243 a         6397 c         6104 g         5895 t
ORIGIN      
        1 taatatcaat aaaaaaagct taaatcattc atataaatga tttaagcttt ctaaatccga
       61 ttaaaaccag ggtggcaaat taatcgtagc gatttaacac gattcgacgt ttgctatcgc
      121 tatctgcagc aatcacttcc gtcgttacac ctttaactgg cgaagaaacc tgatgacggg
      181 tatcaacatt atacgttaac tgaccacaat gctgttgagg ttgaccatta accatagcca
      241 tactgcatgt tgggggactg atcctaccat taatcgttaa tgtagcagtt tctgaagaga
      301 acgcgggaga agccaatcca taggcaagaa aaataattcc tgacagccag tacttattca
      361 ttgaacgtta tccctgtagt aaaggttatg cctggaaaga atgagtgcat aacaaactat
      421 agctgtacat ccactacaca gccacgaagg atgataatga agcatcgccg gtacgatcaa
      481 tcgactttgt agagtttcgg acgaaggtcc gcagaatatt cgcagcatta aataagtgtt
      541 cattaatgcg gcaaaaacaa atacaccgca tcaatgtaac atctctgcgg tgtatgaaga
      601 ataatcagaa tggttaatac gcgttagacc gcgcggaagg aaatctcgcc tggaatgact
      661 tcaccctgcc agtagagctg agctgcaacg cggtcagcca gctggcggta gatagcggta
      721 aattcgctct caggacggct gataaccgtt ggcgtgcctt tatcgagatc ctcacgtaag
      781 gagatgtgta acggcatctg ccccagcagc tgcgtgtggt atttctcggc cagtttctct
      841 gcaccaccgg taccgaaaat cggttcatgg tgaccacagt tactgcaaat atgcacactc
      901 atgttctcga caataccgag caccggaact tcgactttct cgaacatcac aatgcctttt
      961 ttcgcatcga tcaacgcgat gtcctgcggg gtggtgacca cgactgcacc ggttactgga
     1021 atgttttgtg ccaatgtcaa ttgaatgtca ccggtacctg gcggcatatc aagcaccaga
     1081 tagtcgagat ccggccacaa ggtttcctgc aacatttgca ttaacgcctt gctcgccatt
     1141 gggccacgcc acaccatcgc attgtcgtct gtcaccagat aaccaattga attagttgcc
     1201 aggccatgag acataatcgg tgccatatgg gtaccgtccg gcgaagttgg acgctgattt
     1261 tctgcgccca gcatggtagg gattgatggg ccgtagatat cggcgtccag aatgccgact
     1321 ttcgcccctt ccgccgccag tgccaacgcc aggtttaccg ccgtggagga tttacccacg
     1381 ccacctttac cggaactgac cgcgataatg ttcttcacgc cgttaattcc cggctgattc
     1441 ttcacgcgtt tcagagtggc tatgttatgc gacagtttcc agtcgatagc tttcgctccg
     1501 gtgatgcgca gcagttcggc gctacattgc tcttttaact cttcgaacgc gctatgccag
     1561 acgaatggca taaccagttc cacatgcagc gtgtcgtcca tccaggcgac atggtgcaac
     1621 gctttcagcg ttgtgaggtt atgcttcagg gttgggtgct gaaaattggc cagtgtcccg
     1681 gcgaccattg cgcgcaaggc ttctggtgat ttggcctggg attgttcgtt catcccgact
     1741 ccttttttgt atagataaac catcagttga tagtttacct gaagaatata gagaagtact
     1801 tacttaacat tttcccattt ggtactatct aagccctttt cactattaag aagtaatgcc
     1861 tactatgact caagtcgcga agaaaattct ggtgacgtgc gcactgccgt acgctaacgg
     1921 ctcaatccac ctcggccata tgctggagca catccaggct gatgtctggg tccgttacca
     1981 gcgaatgcgc ggccacgagg tcaacttcat ctgcgccgac gatgcccacg gcacgccgat
     2041 catgcttaaa gctcagcagc ttggtatcac tccggagcag atgattggcg aaatgagtca
     2101 ggaacatcag actgatttcg caggctttaa catcagctat gacaactatc actcgacgca
     2161 cagcgaagag aatcgccagt tgtcagaact tatctactct cgtctgaaag aaaacggttt
     2221 tattaaaaac cgcaccatct ctcagctgta cgatccggaa aaaggcatgt tcctgccgga
     2281 ccgttttgta aaaggcacct gcccgaaatg taaatccccg gatcaatacg gcgataactg
     2341 cgaagtctgc ggcgcgacct acagcccgac tgaactgatc gagccgaaat cggtggtttc
     2401 tggtgctacc ccagtaatgc gtgattctga acacttcttc tttgatctgc cctctttcag
     2461 cgaaatgctg caggcatgga cccgcagcgg tgcgttgcag gagcaggtgg caaataaaat
     2521 gcaggagtgg tttgaatctg gcctgcaaca gtgggatatc tcccgcgacg ccccttattt
     2581 cggttttgaa attccgaacg cgccgggcaa atatttctat gtctggctgg acgcaccgat
     2641 tggctatatg ggctctttca agaatctgtg tgacaagcgc ggcgacagcg taagcttcga
     2701 tgaatactgg aagaaagatt ccactgccga gctgtatcac ttcatcggta aagatatcgt
     2761 ttacttccac agcctgttct ggcctgccat gctggaaggc agcaacttcc gcaagccgac
     2821 caacctgttt gttcacggct atgtgacggt gaacggcgcg aaaatgtcca agtctcgcgg
     2881 cacctttatt aaagccagca cctggctgaa tcattttgac gccgacagcc tgcgctacta
     2941 ctacaccgcc aaactgtctt cgcgcattga tgatatcgat ctcaacctgg aagatttcgt
     3001 tcagcgcgtg aatgccgata tcgtgaacaa agtggttaac ctggcgtccc gtaacgctgg
     3061 ctttatcaat aagcgttttg acggtgtgct ggcaagcgag ctggctgacc cgcagttgta
     3121 caaaaccttc actgatgccg ctgaagtgat tggtgaagcg tgggaaagcc gcgaatttgg
     3181 taaagctatc cgtgaaataa tggcactggc tgacctggct aaccgctatg tcgatgaaca
     3241 ggctccgtgg gtggtggcga aacaggaagg ccgcgatgcc gacctgcagg caatttgctc
     3301 gatgggcatc aacctgttcc gcgtgctgat gacttacctg aaaccggtac tgccgaaact
     3361 gaccgagcgc gcagaagcat tcctcaatac agaactgacc tgggatggta tccagcaacc
     3421 gctgttgggc cataaagtga atccattcaa ggctctgtat aaccgcatcg atatgaaaca
     3481 ggttgaagca ctcgtggaag catcaaaaga ggaagtaaaa gccgctgccg cgccagtaac
     3541 tggcccactg gcagatgatc cgattcagga aaccatcacc tttgacgact ttgctaaagt
     3601 tgacctgcgc gtggcgctga ttgaaaacgc agagtttgtt gaaggttctg acaaactgct
     3661 gcgcctgacg ctggatctcg gcggtgaaaa acgcaatgtc ttctccggca ttcgttctgc
     3721 ttacccggat ccgcaagcac taattggtcg tcacaccatt atggtggcta acctggcacc
     3781 acgtaaaatg cgcttcggta tctccgaagg catggtaatg gctgctggtc ctggtgggaa
     3841 agatatcttc ctgctaagcc cggatgccgg tgctaaaccg ggtcatcagg tgaaataatc
     3901 ccccttcaag gcgctgcatc gacagcgcct tttctttata aattcctaaa gttgttctct
     3961 tgcgattttg tctctctcta acccgcataa atactggtag catctgcatt caactggata
     4021 aaattacagg gatgcagaat gagacacttt atctatcagg acgaaaaatc acataaattc
     4081 tgggcggtgg agtagcaagg caacgagtta catatcagtt ggggcaaagt cggcactaac
     4141 gggcaaaacc aggtaaaaag ctttgctgat gctgcggcgg cggaaaaagc ggaactgaag
     4201 ctgattgcgg agaaggtgaa gaagggatat gtggagcaag cgaaggataa ttctttgcaa
     4261 ccttcccaaa cggtaacgga ctctctcaag gtagcggact tatccaccat tattcaggaa
     4321 caaccctctt tcgtagcaga aacccgtgcg gctgacaaaa atacagatgc tgttttacca
     4381 tggctggcga aagatattgc tgtcgttttt ccgcccgaag ttgtacacac cacgttaagt
     4441 catcgccgct ttcccggagc tcctgttcag caagcagaca aattgaccca attacgtcgc
     4501 ttagcctgta gtgtatcgca acgggataat aaaacagcca catttgactt cagcgcctgt
     4561 tctttagaat ggcaaaacac cgtcgcacag gcgatcagtc agatcgacgg cctgaaaaca
     4621 acacagctac catcacccgt aatggctgta ctcacggcac ttgaaatgaa atgcacaaga
     4681 tataaagagc gtgaggatgt tatggatcag attattcagg aaggcggtct ggaatatgct
     4741 actgatgtaa taatacatct tcaacaaata tctattgaat gggattatgt caacaataat
     4801 attgttttcc tgtcgtctgg catttcacct gattacttgc agcaatattc cagctttgaa
     4861 ttacgcctac gtaaacattt atcactggcg gaagagtctc tctggcaaaa atgtgcacaa
     4921 aaacttattg ccgcaattcc acatattcca gaatggcggc aaccattaat tgctttgtta
     4981 ttacccgaaa aaccagaaat tgcacatgaa atttcccagc gtctactggg gcaaaagaaa
     5041 ttaccctcgc ttgagtggct aaaaatagtg gcgactgatg agcacattct tgcctcatta
     5101 gaaaaatatc acgagccata tgccattttt gatgattact attgtggtgc gatatggtca
     5161 gccaccgtat tacaggagca aggtgttaca gccctgcccc gatttgctcc ctatgccgca
     5221 agtgactact gcgccgatgt gttgcgtcat atcaatcatc cgttcgcatt aacactgctt
     5281 atacgtgtag ccgggcatac taaacgctgt cacgatcgaa tgacgaaagc ctgcgctgcg
     5341 ttcccacacg cagcactggc agcactggcg gaacttctag tgcaaaaaga agagaatagt
     5401 tggcgcatta tgctaatgac gatgcttatc tcgcatccga cactagcaga acaggtcatt
     5461 ccctggcttt cgacacccgc agttgcagtg ctgaaatcat gtcagcaaca actgacacag
     5521 ccctcaaacc atgccagcgc cgatctactg ccggccatag tagtctcccc accatggctt
     5581 tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag caccgctcaa cctcgaatcc
     5641 atatgcacaa taaccgacac agaagctaaa gagtttcaaa ctcattggga ctgggaaccg
     5701 cataagccaa gtgaaggtgc taaaaatttc ctttactcgc tggggtatcg ccgctgggat
     5761 tttgacacat ataaatatat cggcgcttct gactcggcaa ttgacgcatg ggaacgtgag
     5821 gacttcgcta cactcatcca aatgtttaaa gcccaccatg caccgtatca gggggagtgg
     5881 catctcaact cacttccctt tttacctatg caaaaagcga ttaagctatg ggaatttctc
     5941 agcaaagagc cgcataccgc gataaaacca gtcatgcttt atttgcgact ggcaggtatg
     6001 agtggttttc tacactcgtt ttcacgctac cctcaagaag gttttgcggt tgctaattat
     6061 tttgccgcaa cggaactggc tcctgccgtc gcccgcgcct tcaacaaact caaaacatta
     6121 cgacaagacg ccagtagctg gctgctgaaa tacccggaac acgccattac cggcctgctg
     6181 cctgcagcgc tcggcaaagc cagtgaagca caggacaacg cccgtgctgc cttgcgtatg
     6241 cttaccgaaa acggtcatca gccattgctg caagaaatcg cccggcgtta taaccagccg
     6301 gaagtaaccg atgcggtgaa cgctctactt gcgctcgatc ccttagataa tcacccgaca
     6361 aaaatcccca ctcttccgac cttttatcag ccatcgctct ggacgcgccc gttattaaaa
     6421 gcaaacgccc aatcactgcc tgatagcgca ctcctccacc tcggtgaaat gctccgcttc
     6481 cctcaggaag aggcactgta tccgggatta ttgcaggtga aagatgcctg taccaccgac
     6541 tcactggctg aatttgcctg ggatctgttt accgcctggc agaccgctgg cgcgccgtcg
     6601 aaagagagtt gggcgttcac tgcgttaggc gttctcggta acgatgacac cgcccgcaaa
     6661 ctgacgcctt tgattcgcgc ctggcctggt gaatcccagc ataaacgcgc caccgtcggg
     6721 ttggatattc tcgctgctat cggtagtgat atcgccctga tgcagcttaa cggcatcgcc
     6781 cagaaactga aattcaaagc attacaggag cgggcaaaag aaaaaattgc cgacattgcc
     6841 gagagccgcg aactcacggt ggcggagctt gaagatcggt tagcaccgga tctcggtctg
     6901 gatgataatg gttcgttgtt gctggatttc ggcccacgcc agttcaccgt cagctttgat
     6961 gaaaccttaa aaccgtttgt gcgtgacgct tccggcagtc gtctgaaaga cctgcccaaa
     7021 ccaaacaaaa gcgatgatga atcacaggcg aacgatgctg ttaaccgcta caaattgctg
     7081 aaaaaagatg cgcgtaccgt cgccgcccag caggtagcaa ggctggaatc cgccatgtgc
     7141 ctgcgtcgcc gctggtcacc ggaaaacttt cagctcttcc tggttgagca tccgctggtc
     7201 cgccacttaa cccgccgtct gatttggggc gtttatagcg ccgaaaacca gctactggct
     7261 tgctttcgcg tagcggaaga taacagctac agcaccgctg acgatgatct tttcaccctg
     7321 ccggaaggcg atatctctat tggtattcct cacgttctgg aaatatcacc gacggatgct
     7381 gccgccttcg ggcagctttt tgccgactac gaactgttac caccgttccg ccagcttgat
     7441 cgtaacagtt acgcgctgac agaagccgag cgcaacgcca gcgaactgac acgctggaca
     7501 ggcagaaaat gcccgagtgg tcgggtaatg gggctggcga ataaaggctg gataaagggc
     7561 accccgcagg atgctggctg gatcggctgg atgatcaccc ccctgggccg ctggtcgtta
     7621 atcatggaaa tcgatgaagg ttttgcggta ggcatgtcgc cagccgaact gagtgccgag
     7681 caactcttaa gcaagctgtg gctatgggaa ggcaaagcgg aaagctatgg ctgggggagt
     7741 aattcaacac aggaagcgca gttctccgta ctcgatgcca tcaccgccag cgagctaatt
     7801 aacgatattg aagccctgtt tgaataagga aagagcatgg acaaggaatt accgtggctg
     7861 gcggataacg cccaactgga acttaaatat aaaaaaggca aaacaccact cagccatcgc
     7921 aattggccag gcgaaccggt gcctgttatc actgaaagca tcattcagac actgggtgat
     7981 gaattgctac aaaaagcaga gaagaaaaaa aacattgtct ggcgttatga aaacttttca
     8041 ctggagtggc agtccgccat cacgcaggcc atcaacttga tcggcgaaca caaaccctca
     8101 atcacggccc ggacaatggc ggcgctagcc tgtatcgcgc aaaatgatag ccaacagttg
     8161 ctcgacgaaa tcgtccaaca agaggggctg gaatatgcaa ctgaggtggt gattgcacgc
     8221 cagtttattg tacggtgtta tgagagtgat cctctggtag taacattgca atatcagaac
     8281 gaggactatg gctatggtta tcgctcagaa acctataacg aattcgatct ccgattgcgt
     8341 aagcatctct ctctggcaga ggaaagctgc tggcagcgtt gcgtcgacaa actcattgcc
     8401 gcactgccag gaataaccaa agttcgccgc ccttttattg cgctgaacct cccggaaaaa
     8461 cccgaaatcg ccaacgagct ggtaagcctt gaatgttcgc aaactcattt tcgctcaaaa
     8521 gagtggttaa aagttgttgc tgatgaccct aaggcggtga aagaactcgc acgctactgg
     8581 agccaggata tatttagcga tcgagaagcc agctacatgt cgcatgaaaa ccacttcggt
     8641 tacgcggcct gcgctgccct tttgcgcgaa caaggactgg cagccgttcc gcgcctcgcg
     8701 atgtatgcac ataaagaaga ttgcggcagt ctgctggtac aaattaacca tccgcaagtc
     8761 atccgcacct tgctactggt ggctgataaa aacaaaccca gcctgcaacg tgtggctaaa
     8821 tacagtaaaa acttccccca tgcgacgctc gccgcactgg cagaactgct ggcgttaaaa
     8881 gccccccctg cgcgccctgg ttatcccatc atcgaagaca aaaagctgcc tgcacagcaa
     8941 aaagcacgtg atgaatactg gcatacgctg ttacaaacgc tgatggcatc gcagccacaa
     9001 ctggcagaag aggtgatgcc gtgcttaagt acacaagccc aggcagtggt gaatggttat
     9061 ttatcggcat cgccaaaact tgctttcgag agtactcata gcaacgacct gccagaaata
     9121 cttgtttctc caccgtggcg aggtaagaaa aaaacggcat tgctgcgcct tgatttagtg
     9181 cctcttgaat tagcgcccaa agcccgctgg caaccaggcg aacgagagag acttgccgcc
     9241 acagagtctg cccgttattt cagcacggga tcttttacgg aacgcatgga acgaaaaagt
     9301 ggtcgagttg tactacagga gctaggtttt ggggatgatg tatggctgtt ccggaattat
     9361 atactgcccg gcaaactgga tgcggcgcgc aaatcattag tgggtcagtg gcattactcc
     9421 ccgcgcagag tagaggaaat caacaatggc tggcactcca cggaagcaaa atcagccgaa
     9481 caggcgcttc gttccggcga cgttgaagcg ttaattaaca cctgggaaaa tgatagctac
     9541 tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt tattggcaca gttgccgcgt
     9601 gagatggctt tgactttctg gctgcgtatc aatgagaaaa agcatctgtt cgcgggtgag
     9661 gactattttc tcagtatcct cgggttggat acattacctg gcctgttatt ggctttttca
     9721 catcatccaa aagaaacatt tccgttaatt ttaaatttcg gcgcaacaga actggccctg
     9781 cccgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg atctggctcg ccagtggatt
     9841 ttacaatggc cggaacatac ggctactgca cttatccctc ttgtctttac aaaacccagc
     9901 gataatagcg aagctgcatt acttgccctg cgtttactgt atgaacaagg gcatggcgaa
     9961 ttactgcaaa ccgtggcaaa ccgctggcag cgtacagatg tatggcctgc cctggagcaa
    10021 ttacttaaac tgggtccaat agaaatttac ccggcacgta ttccaaaagc tcctgatttc
    10081 tggcatccgg caatgtggtc caggccgcgc cttatcaaga ataatcagcc tgttaccgat
    10141 gacgctctgg aaattatcgg cgaaatgctg cgctttaccc agggcggacg tttttatggc
    10201 ggactggaac agctgaaaac gttttgccag ccgcaaacgc tggcagcttt tgcctgggat
    10261 cttttcaccg catggcaaca agctggagcc cccgcaaaag acaactgggc atttctggcg
    10321 ttaagtctct ttggtgacga aagcacggct cgggatctga cgacacagat cctcgcctgg
    10381 ccacaagaag gcaaatctgc ccgtgctgtc agtggcctga acatccttac cctgatgaat
    10441 aatgatatgg cgctgataca gctgcaccat atatcccaac gggcgaaatc ccgctcctta
    10501 cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa atcgcgggct aagccaggaa
    10561 gagttagcgg acagattagt cccaacactg ggtcttgatg atccgcaggc gttgattttt
    10621 gattttggtc cacggcaatt taccgttcgc ttcgatgaaa acctcaaccc ggttatcttt
    10681 gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc gcgccgatga cgatcaactg
    10741 aaagcccccg aggcactggc ccgactcaaa gggctaaaaa aagatgcgac tcaggtgagc
    10801 aaaaacctgc tcccgcgtct tgaaactgcc ctgcgcacca cccgacgctg gtcgctggca
    10861 gattttcatt ctctgtttgt taatcatccc tttacccgtc tggttactca gcgattaata
    10921 tggggggttt atccggcaaa tgaaccgcgt cgtttactta acgcctttcg tgtggccgct
    10981 gagggggagt tctgcaatga gcaagatgag ccaattgacc tgcctgccga tgctctgatt
    11041 ggcattgccc acccgttaga aatgacagca gaaatgcgca gtgaatttgc acagcttttt
    11101 gccgattacg aaattatgcc gccttttcgc cagttgtcgc gccgcacggt gctgctcaca
    11161 cctgacgagt cagccagtaa cagcctgact cgctgggaag gtaaatccgc taccgttggt
    11221 caacttatgg gaatgcgata caaaggctgg gagtcaggct atgaggacgc atttgtctat
    11281 gacttaggtg cgtaccggct ggtccttaag ttttcacccg gttttaacca ctacaatgtt
    11341 gatagcaaag cgctaatgag cttccgttct cttcgggttt accgtgacaa taaatccgtc
    11401 acttttgccg aacttgatgt gtttgatttg agtgaggcat taagcgcacc tgacgtcatt
    11461 ttccattaaa acacagcggg cagtgatgca actgcccgtt atcaacacga cctaccagcg
    11521 ggggaagcga tgatcgtgca aaaagagctg gttgctattt acgattatga gatccctgta
    11581 cctgaagagc cgttttcctt cagacttgaa atccataaat gctctgaatt atttacaggt
    11641 tccgtctatc gactggagcg attccggcta catccaacat ttcatcaacg tgatcgagaa
    11701 gatgccgacc actaataaat gatgcgttta tttatataag agatgagttt attgatgagc
    11761 ggaaattacg aggtgaatca catgaaacag taatagcaat ttttaattgt gaactacaga
    11821 atatattcaa ccaagaaata gaataatata ctctagataa ttcaagtcgc aggaaggcga
    11881 taagcgagtg aatccccagg agcttactaa agtaagtgac aggggtgaac gagcgcagtc
    11941 gcagcacatg caacttgaag tatggcgagt aaagctttat taaaggggga aacggtattc
    12001 ccccttagat gcgtttcaat ttgcccgtta cttcaacaaa tcaatcaacc gtaacagagc
    12061 cactaatagc tccagtacag caatgagcac ggttaacttt tgtgtcagcg tgtccatccg
    12121 ctctccttat taaggagcgc aacaccagca ccttaccctt acactgcctg tcgatactgg
    12181 tttacgtatg ttggaccacg atgttgcact ccggatcagg gcaccacctg cagcaccacc
    12241 cgtccggtag ccaggacttg aaattggttc tatccggcgc aacctcccaa cagtcaggat
    12301 tataaatctc attatcttaa ttgcaacggg gtccagcggt ggttataatt cgttcgttgg
    12361 aacacgatgt tgcacaggct gcggtgttag gcctgaaagt agtaaataaa agaacctgcc
    12421 tcaccagcag gtttttttat tttttgcgat ctgctttcca gatattttcc gcatcaaaca
    12481 actaatgcgc caaacagtta ttgcgcgtaa aatatcgttt atttcattaa tacatttcag
    12541 ggatgaatat atgtcaccgc agaataatca cctgcagcgt ccgcctgcta ctgtgttata
    12601 cgccgttgaa ctggcaaaat taaaacaaaa tgataacgca ccttgcccgc caggttggca
    12661 gttaagtttg cctgcggccc gtgcttttat ccttggcgat agcgcgcaaa atattagccg
    12721 taaagtggtg attagtccct ccgctgtcga acgtatgtta gtcactcttg ccaccgggcg
    12781 cggtttgatg ttggtggggg aacctggcac cgcaaaatct ctcctgtctg aattactggc
    12841 aaccgctatt agcggcgatg ccgggttaac catccagggc ggcgcatcta ctactgaaga
    12901 tcaaatcaag tacggctgga actacgccct gcttatcaac catggtccgt caacggaagc
    12961 actggtccct gccccgcttt accagggaat gcgcgacggc aaaatcgtcc gttttgaaga
    13021 gataacgcgt acaccgctgg aagtacaaga ctgtctgtta ggtatgcttt ctgacagggt
    13081 gatgacggtg ccggaactca ccggtgaagc cagtcagctc tatgcgcggg aaggctttaa
    13141 tattattgcc actgccaata cccgcgatcg cggcgtcaat gaaatgagtg ccgcgctaaa
    13201 gcgtcgcttc gattttgaaa ctgtctttcc gattatggat ttcgcccagg agctggagct
    13261 tgtcgccagt gcttcggcgc gtttgctggc ccatagcggc attccgcata aagtgcctga
    13321 cgcggtactg gagttactgg tccgcacctt tcgcgatctg cgtgccaacg gcgaaaagaa
    13381 aacgtcaatg gatacactga cagcgattat gtccaccgct gaagccgtga acgtggccca
    13441 cgcggtgggc gtccgcgcct ggtttttagc gaatcgcgca ggtgagccag ccgatctggt
    13501 ggactgtatt gccggaacca tcgtcaaaga taacgaagaa gatcgcgcgc gcctgcgccg
    13561 ttacttcgaa caacgtgttg ctacacataa agaagctcac tggcaggctt attatcaagc
    13621 tcgccaccgc ctgccgtgag gaaagatgta tgagcgagcc gttaattgtc ggcatccggc
    13681 atcatagccc ggcctgcgcc cggctggtga aattgttaat cgaaagccag cggccacgat
    13741 acgtattgat tgaaggcccg gctgatttta atgaccgggt ggacgaactg tttttagccc
    13801 accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgcg gcccccgggc
    13861 gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta caagccgcac
    13921 gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag agtgaagaag
    13981 aggacgattc gcctgataca caagatgaaa gccagacctt actgctgcgt gccacccgca
    14041 tggataacag cgataccctg tgggatcact tattcgaaga tgaaagccag caaactgcat
    14101 taccctctgc actggcgcgc tattttgccc aactgcgggg cgattacccc ggagatgctc
    14161 tcaatcgtca gcgcgaagcc tttatggctc gctggattac atgggcgatg cagcaaaaca
    14221 atggcgacgt gttagtcgtc tgtggcggct ggcacgctcc tgcactggca aaaatgtggc
    14281 gcgaatgccc gcaggaaatt aacaagccag aattgccctc gctggcagat gccgttactg
    14341 gttgttatct cacaccctac agtgaaaagc gccttgatgt gctggcagga tacctttccg
    14401 gaatgcctgc cccggtctgg caaaactggt gctggcagtg tggcttacag caggcaggtg
    14461 aacagctgct gaaaacggtt cttacccgtt tgcgccagca caaattgccc gcttctaccg
    14521 ctgatatggc tgctgctcat ctgcatgcta tggcactggc acagttgcgc ggccatacac
    14581 tacctctacg cactgactgg ctggatgcca tagctggttc gctgattaaa gaagccttga
    14641 acgcgccgtt gccgtggagc taccgcggag tcattaatcc cgataccgat ccgattctga
    14701 taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct tctacgccac
    14761 aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccggaatc tctcttccgg
    14821 cggagcttac cttaaatcgc tttacccccg atggactggc gcaaagtcag gtgttacatc
    14881 ggttggcaat actggagatc ccaggggttg tgcgccagca gggaagtaca ctgacacttg
    14941 caggcaacgg tgaagaatgc tggaaattaa cccgcccgct tagccaacat gcggcattaa
    15001 ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgcaataaa ttagaagccg
    15061 atatgctgga cgcgggtgga atcggctgta tcaccacatg tcttagccag gcagcgttag
    15121 cgggtctggc gtctttcagt cagcaattac tggaacaact cacattatta atcgcccagg
    15181 aaaatcaatt cgccgaaatg ggccaggcgc tggaagtgct atatgcctta tggcggctgg
    15241 atgaaattag cggtatgcaa ggcgcgcaga tattacagac gacgttatgc gcggctatcg
    15301 atcgcacact ctggctatgt gaatccaacg gcagaccgga tgaaaaggag tttcacactc
    15361 acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc ggcgttaatt
    15421 tacccggcgt ttcgctttct gcggcggtag ccttactgga gcgacacagt caggcgattc
    15481 atgtcctggc actggatcgc ggcgcgactc ttggtgcgct gatgcgtctg gaacatccca
    15541 acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccgaca caatccggcg
    15601 aggcgctgca cggtttgctg gcattagccc gtcatcaact ggcctgtcag ccggtattta
    15661 tcgccggttt cagcagtcat ttaaatcaac tgagtgatgc cgattttatc aatgccctgc
    15721 ccgatttacg cgcggcgatg gcctggctac caccacgaga acgcgggacg ctggcgcatc
    15781 aggtgcttga gcattatcaa ctggtgcaac ttcccgtttc ggcactgcaa atgccgttgc
    15841 attgtccacc acaagacatt gcacatcatc aacaactcga acagcaggca ctggcatcgc
    15901 tgcaacactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac ccgtgagcta
    15961 caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg gctggatgac
    16021 aacgcccggc agatagacca cgcgctggag tggctgtatg ggcgcgatcc tgaacggctc
    16081 cagcgtggtg aacgttacgg tggattaggt cgttcaaatc tcaccacccc tgagtggatc
    16141 aacagcattc acacgctgtt tccgcaacgg gtgatcgagc ggctggaaag cgatgccgtg
    16201 ctgcgctacg gcattgaaga tgtggtgacg aatctcgacg tgctggaacg tatgcagcct
    16261 tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc cgaagtactg
    16321 gctgccgccc gccggatagt gcaccaggtt gttgaagaga ttatggcgcg actggcaaag
    16381 gaagttcgtc aggctttttc tggagtacgc gatcgccgtc gccgctcatt tatttcactg
    16441 gcgcgaaact ttgatttcaa aagtactctg cgcgccaacc tgcaacactg gcacccgcaa
    16501 cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg ccatagcgaa
    16561 caatggcaac tggtcctact ggttgatcaa agcggatcga tggttgattc ggtgatccac
    16621 tccgcagtga tggcggcttg tttatggcag ttacccggca ttcgtaccca tctggtcgct
    16681 tttgacacca gcgtggttga tctcacggca gacgttgccg atccggtaga gctattaatg
    16741 aaagtgcaat taggcggcgg gaccaatatc gccagtgctg tggagtatgg tcggcaactg
    16801 attgaacaac cagcgaaaag cgtcattatc ctcgtgagcg atttttacga aggggggtca
    16861 tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa agtgctggga
    16921 ctggcggcgc tcgatagcag cgcaacacct tgctatgacc acgatacggc ccaggcgctg
    16981 gttaatgtcg gcgcacaaat agccgcaatg acaccgggcg agctggcttc ctggcttgcg
    17041 gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca ccacaggcac
    17101 tgacggcgtt aagcaatgcc ggttttgtta agcgcagcct taaggaactg gaaaatggca
    17161 acgtcccgga aatcagtcat gagaacggcg ctttaatcgc caccttcagt gacggtgtcc
    17221 gtacccagct ggcgaacggc caggcactga aagaggctca gtgcacctgc ggggccagcg
    17281 gcatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt gccaccgccc
    17341 agcctacgga aaaaaaagaa gaagagtggg atccggcaat ctggctgaaa gaactggcta
    17401 ccttgcccga tgccacccgc aaacgcgcgc aggcgctggt cgctaaaggc atcaccattg
    17461 agttgttctg tgcgcctggc gaaattccct ctgcccgatt accgatgagc gatgtgcgtt
    17521 tttattcccg cagcagcatt cgcttcgcac gctgtgattg cattgaaggc acactttgcg
    17581 aacatgtcgt actggctgtg caggccttcg tcgaggccaa aactcagcaa gcggaattta
    17641 ctcatttaat ctggcagatg cgcagtgaac acgccacatc atctgatgct ccgtttgcca
    17701 gcgaagaagg caaaacgtgt cgtcaatatg ttcagcaatt aagccaggca ttatggctgg
    17761 gcgggataag tcaaccgcca atccattacg aggccgcttt cagtcgcgcg cagcaagcgg
    17821 cggaacgctg caactggcga tgggtgagtg aatcgctacg gcaattgcgc gccagtgttg
    17881 atgccttcca cgcccgtgcc agccactacc atgccggaga atgcttacgt cagcttgcgg
    17941 cattaaacag tcgattaaat tgcgtacaag agatggcgcg acgcgacagt atcggtgaag
    18001 ttcctcccat gccgtggcgc acggtcgttg gcgctggtat tgccggagaa gcaaaacttg
    18061 atcatctgcg gctggtgtct ttaggtatgc gttgctggca ggatattgag cagtatggtt
    18121 tacgcatctg gtttaccgat cccgacacag gaagtatttt gcatctttcg cgtagttggc
    18181 agagaagcga acaggaaaac tcaccggcag cgacgcgtcg gctgtttagt tttcaggctg
    18241 gcgcactggc aggtgggcaa attgtttcac aagctgcaaa acgcagtgcc gattgcgagc
    18301 tgctgttcgc gacccgtaac cgcttgagtg gcgttgttcc actgtcgcca gacgcctggc
    18361 taatgttgag cgcgccgttg cgccagccgg gcattgtggc tttgcgggaa tatttacgcc
    18421 agcgtccccc cgcctgcata cgaccactca accaggtcga taacttattt attctgccgg
    18481 tagcggagtg tatttcgctc ggttgggata gcagccgcca gacactggat gcgcaggtaa
    18541 tcagcggcga aggggaagat aatgtgctga cgttatccct tccggcatct gcctgttctc
    18601 cttttgccgt tgaacgcatg gcggcgcttt tgcaacaaac agacgacccc gtgagcctgg
    18661 tttctggctt tgtcagtttt gtagaggggc aattaacact ggaaccacgg gtgatgatga
    18721 caaaaacccg cgcctgggcg ctggacgcag aaactgcgcc tgtggcaccg ctaccttctg
    18781 ccagcgttct gcctgtgccg tctaccgctc atcagttgct gatgcgctgc caggcgttac
    18841 ttattcaact gctccataac ggctggcgct atcaggaaca gagtgctatt ggtcaggcag
    18901 agttgctggc gaatgacctc accgcggtgg gtttttatcg gctggcacat gtgttgggac
    18961 aatttcgtaa tacagaaagc gaggcacggg tagaagcaat gaataacggt gttttgcctt
    19021 gcgaacaatt attccccatg cttcagcaac aaggatgaaa tagtgctttt tattaagagt
    19081 tctactcctg ttccggactg ctcacgccac agtattaggc atatcctata tagcccctgg
    19141 tgagagtcac cagttccttg attaaataaa atggagtttt acatgaaggc tttcaataag
    19201 ctgttttccc ttgttgttgc atctgttctg gttttctctc ttgctggctg cggtgacaaa
    19261 gaagaatcaa agaaattcag cgccaatctg aacggcactg aaattgccat tacctatgtc
    19321 tacaaaggtg acaaggtgct taagcaatct tctgaaacca aaattcaatt tgcctccatt
    19381 ggtgcaacca ccaaagaaga tgccgccagg acacttgagc cgttaagcgc taaatacaaa
    19441 aacatcgcgg gtgttgaaga aaaattaacc tataccgata cctacgcgca ggaaaacgtg
    19501 actattgata tggaaaaagt ggattttaaa gccctgcagg ctatttcagg aatcaacgtt
    19561 tctgctgaag atgctaaaaa aggtatcact atggcgcaaa tggaactggt gatgaaagcc
    19621 gctggtttta aagaagtgaa ataatctgtc ggcggccatg tttcgcatgg ccgccatacc
    19681 cgtcttagct tttcttcaca tgctggcgcg ctgccagtcc acgcagaaaa taacgtaaaa
    19741 attgatcgcc gcattcgcgg aagtttttat gatccggtgc acgcatcatc gccgtaattt
    19801 ccggcatcga aacgcggaac tgctgttcgg tgaggattgc cagaatgtca tcggttttaa
    19861 gcgaaaacgc gatgcgtaat tttttcagca cgatgttgtt attaatgcga cgttccggct
    19921 ccagtgccgg agcagactca tccttgccgc gtttttcata aatcaggcca ttgaggaatg
    19981 aagacaaaac aatgtccgga caacgctgaa aaccctcttc gtcttcttta cgtagccaga
    20041 cggcgatctg ttccgcggtg gcttcgacat tacccagcgc cagaatacgc accaggtcat
    20101 tattattggc tttcaaaatg tagcgcacgc tgcgcagaat atcgttactt agcatgaggc
    20161 cttcaggtgt tgatgaggca aaaagccatt ttagcagtct tttacaggcc aatcgcctct
    20221 tttaagcttt tcagataacg gcggctgacc ggcacggtta agccattacg caaaattaac
    20281 tcggcctggc cgttatcttc cagacggatc tcctgtaaat gcgcgaggtt aaccagatac
    20341 tgacgatggc agcgcagtaa tggtgtacga ctttccaggg tacgtaatgt cagttcggta
    20401 aagccctctt tcccttcgtg actggtaacg tagacaccgc tcatccgact gctgacaaat
    20461 gccacatctt tcatttgcag caaataaatc cgactatgcc ccgtacaagg gataaatttc
    20521 agcgcctgtt gattttccgg taacagcgaa acatcctgct tgctgcgctc ctgacgcaat
    20581 cgcgccagcg ttttctccag tcgcgcttca tcaattggct tcagcagata atcaaaggca
    20641 tgttcttcaa aggctttaat ggcgtattcg tcaaacgcgg tgagaaaaac aatatacggg
    20701 cgatgttccg gatcaagcat ccccaccatt tccagaccac tgatgcgcgg catctggata
    20761 tcgagaaaca gcacatccgg gcgcagttta tgcaccgcgc cgatcccttc tacggcgttt
    20821 gaacactctc caacgatttc aatatcgctc tgctcctgca aaaatacgcg caggttctcc
    20881 cgtgctaacg gttcatcatc gacaattaag actttaatca tgcctcgtcc ctccatggta
    20941 gtcgtaacgt tattcgggtg taactatcag gctcacaggc gacgcttatc ccatagtcat
    21001 cgccaaaccg ttcacgtaaa cgcttatcca ccagattcat ccccagccca ctggcattgg
    21061 ttaccggttg atacaaaccg gcattgtctt cgatctccag catcaaatgt tgcccctcac
    21121 gtcgggcgct gattgccact cgccctgtat ccaacagttg tgatgtccca tgtttaatgg
    21181 cgttttccac tatcggttgc agggtaaacg cgggcaattg ctgctgggat aattcttgcg
    21241 gaatagcaat gttgacctgc aaccgcgact ggaagcgcgc cttttcaatt tgcagataag
    21301 cgttcacatg ttcaatttcg tcggcgagag taacaaactc cgaaggccgc tttaagtttt
    21361 tgcggaaaaa agtggaaaga tactgcacca gctggctggc ctgttcgctg tcgcggcgga
    21421 tcaccgcttt aatggtgtta agcgcattaa acaaaaaatg gggattcacc tgggcgtgaa
    21481 gcagtttgat ctctgactgg gtgagcatcg ctttttgccg ctcatattgc ccggcgagga
    21541 tctgcgccga aagcagttgc gcaatcccct cgcccaacgt gcggttgatt gaactgaata
    21601 aacggttttt ggcttcatac aatttgatgg tgcccatcac ccgctgattt tcgccacgca
    21661 acggaatgac cagcgtcgac cccagtttgc attgcggatg caaagagcaa cgataaggta
    21721 cttcgttgcc atcagcgtag accacttcac cggtttcaat cgcttttaag gtataagtcg
    21781 aagaaatcgg tttgccgggt aaatggtggt cgtcaccaat tccggtaaag gccagcaatt
    21841 tctctcgatc ggtaatcgcg actgcaccaa tatccagctc ctgatacagc acctgtgcca
    21901 ctttcatgct gttcacttcg ttaaacccct ggcgcaaaat gccttccgtc gaggccgcca
    21961 ctttcagcgc agtggcagaa aaagccgaag tgtatttttc aaacatcgcg cgtttatcga
    22021 gcaatatacg cataaacaac gccgcgccga cggtattggt gaccatcatt ggcgcagcaa
    22081 tattactcac cagacgcacc gcatcttcat aaggtcgggc gatcgccagg ataatcaaca
    22141 tttgcaccat ttcagcgacg aacgtgacgg caccggcggt aatggggtta aagactttat
    22201 cagtgcgccc gcgacggatc aggatgctgt gtaccaggcc gccgagcaat ccttcaacga
    22261 tggtcgagat catgcaactt agcgcggtca tgccccccat cgaatatcga tgtaacccgc
    22321 cggtcagacc aaccagccca ccgacgaccg gaccgccgag taagccgccc attaccgcac
    22381 ctatcgcacg ggtattggca atagaatcgt caatgtgcaa cccaaaccag gtgcccatga
    22441 tgcagaagat ggaaaagacg atgtagcaga gaaatttatg cggcagacga accgtgacct
    22501 gcattaacgg tatgaataat ggcgttttac tcattaacca cgcaatgact aaaaaaacgc
    22561 acatctgctg aagcagcagc aacaccagat taaaatcgta catacccgca aaccacactt
    22621 ccctttaaaa cgcgtaacat acattgcttg cgtttaactt tctttgaact cttgcagaaa
    22681 aatgagaatt cgtgagtacg atcactcaaa atcgcctggc aaaaataaaa tcaccctata
    22741 gatgcacaaa aaacgggcaa aactacctgg ttcgcaaaac tgcgtctaaa gttaaaccgg
    22801 gacctcgcga gcaagggtga gacgatggcg ctttacacaa ttggtgaagt ggcgttgctt
    22861 tgtgatatta accctgtcac gttacgcgcg tgacgcaaca tttttaaaaa tcgacgaaga
    22921 tcgtccagga gcgccgtttt tcaagggttg gatagatgat tcgtttacca tgtcgacgaa
    22981 cttcgacgaa tttcgccgat aaaaagtgtc ccatacatgc cccattgatg ccccataaac
    23041 aatatcagga attgacactg gttatccata cagacaaaaa taacacctgt acataaccac
    23101 agttacagga gggtgttatg cgtgttgaga tctgtatcgc taaagaaaaa atcagcaaaa
    23161 tgccaaaagg ttctgtttct gccctgcagc aagaaatgct tcgacgtgtc agtaaacgtt
    23221 atgacgatgt ggacgtgatt gtcagagccg ccaacaatga cagtctcacc attttaagag
    23281 ctgtagataa agaatccgcg cacgaatttg ttcaggaaac actgcaggaa gcctgggaaa
    23341 ccgctgacga ctggtttatt cggtaaatct tctttacggg gagttaactt actccccatt
    23401 ttttattgta tgataattgc agcaacggca cagagtgtca ccacaccacc cagccacaca
    23461 aacagcatga agcacattgc tcccgctgta taacgttttc tcccgtacat acggcatttg
    23521 tctttattca tcatctgccc ctgaatcagc tgaagacgct gcaggtccgg taacactgaa
    23581 tcactccgca cacgcaagat gtttcacgct cacaccatac agcccgtttt tcgccattcc
    23641 cgtatacaga aaggtattca ccgtcttcgt gtggataccc atttcactgg caaggcgaca
    23701 gggtctcgac tggcggtaga cataaccgaa catcaccacc atctctgaca tcgtcagcgg
    23761 gcgggcagca ctgttatacg tccagacgcc gttgtgtatg gcatcactta tctgctcacc
    23821 atcacggagc aactccgcac ccagttgcag aaaatgtcgt ccctgaaaca tacagcgcat
    23881 cagcctgccc agtaaaacat cacagattat cagccaggat tcatcaaagt ccgggatata
    23941 acggcggttc tccggcagga aaaactccct cgccactatc acactgtgtt ccttcagcaa
    24001 cgcctgcagg ccatattcca gatagcggtt ccgggttgcc agtatcacct tcatcgcagc
    24061 accttcctgt ttttatgcct catgaaaaaa acggcaggca aaactcctgt gcctgccgtc
    24121 atgcttcatg tcactgtcag caattaaaac tcgaagttca ctcctacgtt gtaggaaaca
    24181 tcaccgtcgc tggccgcaat acctgctttg gctgcggtct gctcgttgaa gcggtaaccc
    24241 acaccaacag ccacagcctg tttgtcactg taaccaccca gggctgccgt ggcgttgaat
    24301 ttacccacac tgtacggctg gaacagaccg gacagcgccg cactctgtgc tgccgcacgt
    24361 ttcatttctt cgtggttttc acgaatctgg cgctgctggc tgttcaggcg tgcgttatgc
    24421 tcctgcagct ggcgagagtg attctggata gcctgggtgt tactgcgggt tctgttgtcg
    24481 aggctgacaa cagcatcatt cacacgaacg ttgcccggtg cgctctggtc aagtgtatag
    24541 ctgccatctt tattctggta gacagtagtg tcagccttaa tatctgcgac tgtttgtttt
    24601 acagaataac gctcaccgct tccctgattt tcatccagc
//
LOCUS       DAZGJT010000070        24636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZGJT010000070 DAZGJT010000000
VERSION     DAZGJT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.070"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..1154
                     /locus_tag="ACHKFK_003071"
     CDS             72..1154
                     /locus_tag="ACHKFK_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HFO9053352.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(1656..2453)
                     /gene="ybcM"
                     /locus_tag="ACHKFK_003072"
     CDS             complement(1656..2453)
                     /gene="ybcM"
                     /locus_tag="ACHKFK_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9053353.1"
                     /translation="MLAKDKSNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQKFYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSIFRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGISFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(2463..3014)
                     /gene="ybcL"
                     /locus_tag="ACHKFK_003073"
     CDS             complement(2463..3014)
                     /gene="ybcL"
                     /locus_tag="ACHKFK_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFO9053354.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKRDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(3183..3515)
                     /gene="emrE"
                     /locus_tag="ACHKFK_003074"
     CDS             complement(3183..3515)
                     /gene="emrE"
                     /locus_tag="ACHKFK_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFO9053355.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGVFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(3860..4174)
                     /gene="fliE"
                     /locus_tag="ACHKFK_003075"
     CDS             complement(3860..4174)
                     /gene="fliE"
                     /locus_tag="ACHKFK_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO9053356.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            4389..6047
                     /gene="fliF"
                     /locus_tag="ACHKFK_003076"
     CDS             4389..6047
                     /gene="fliF"
                     /locus_tag="ACHKFK_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO9053357.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPTNQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEALTREAMGFSEKRGDSLNVVNSPFNSSDESGGALPFWQQQVFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAVKTVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            6040..7035
                     /gene="fliG"
                     /locus_tag="ACHKFK_003077"
     CDS             6040..7035
                     /gene="fliG"
                     /locus_tag="ACHKFK_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO9053358.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            7028..7714
                     /gene="fliH"
                     /locus_tag="ACHKFK_003078"
     CDS             7028..7714
                     /gene="fliH"
                     /locus_tag="ACHKFK_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO9053359.1"
                     /translation="MSDNLPWKTWMPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            7714..9087
                     /gene="fliI"
                     /locus_tag="ACHKFK_003079"
     CDS             7714..9087
                     /gene="fliI"
                     /locus_tag="ACHKFK_003079"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO9053360.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            9106..9549
                     /gene="fliJ"
                     /locus_tag="ACHKFK_003080"
     CDS             9106..9549
                     /gene="fliJ"
                     /locus_tag="ACHKFK_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO9053361.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            9546..10673
                     /gene="fliK"
                     /locus_tag="ACHKFK_003081"
     CDS             9546..10673
                     /gene="fliK"
                     /locus_tag="ACHKFK_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO9053362.1"
                     /translation="MIRIAPLITADVDNATLPGGKASDAAQDFLALLSEALTGEATTD
                     KAAPQLLVATDKPTAKGEPLVSDIVSDAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TITLAAAADNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TVFTKLTSAQLTTAQPDDAPGTPAKPLTPLVAEAQSKAEVISTPSSVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            10778..11242
                     /gene="fliL"
                     /locus_tag="ACHKFK_003082"
     CDS             10778..11242
                     /gene="fliL"
                     /locus_tag="ACHKFK_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO9053363.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            11247..12251
                     /gene="fliM"
                     /locus_tag="ACHKFK_003083"
     CDS             11247..12251
                     /gene="fliM"
                     /locus_tag="ACHKFK_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO9053364.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            12248..12661
                     /gene="fliN"
                     /locus_tag="ACHKFK_003084"
     CDS             12248..12661
                     /gene="fliN"
                     /locus_tag="ACHKFK_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO9053365.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            12664..13029
                     /gene="fliO"
                     /locus_tag="ACHKFK_003085"
     CDS             12664..13029
                     /gene="fliO"
                     /locus_tag="ACHKFK_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO9053366.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            13029..13766
                     /gene="fliP"
                     /locus_tag="ACHKFK_003086"
     CDS             13029..13766
                     /gene="fliP"
                     /locus_tag="ACHKFK_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO9053367.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGINSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            13776..14045
                     /gene="fliQ"
                     /locus_tag="ACHKFK_003087"
     CDS             13776..14045
                     /gene="fliQ"
                     /locus_tag="ACHKFK_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO9053368.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            14054..14839
                     /gene="fliR"
                     /locus_tag="ACHKFK_003088"
     CDS             14054..14839
                     /gene="fliR"
                     /locus_tag="ACHKFK_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO9053369.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            15129..15752
                     /gene="rcsA"
                     /locus_tag="ACHKFK_003089"
     CDS             15129..15752
                     /gene="rcsA"
                     /locus_tag="ACHKFK_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO9053370.1"
                     /translation="MSTIIMNLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKLIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(15796..15984)
                     /gene="dsrB"
                     /locus_tag="ACHKFK_003090"
     CDS             complement(15796..15984)
                     /gene="dsrB"
                     /locus_tag="ACHKFK_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO9053371.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            16147..16374
                     /gene="yodD"
                     /locus_tag="ACHKFK_003091"
     CDS             16147..16374
                     /gene="yodD"
                     /locus_tag="ACHKFK_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO9053372.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            16672..17487
                     /gene="yedP"
                     /locus_tag="ACHKFK_003092"
     CDS             16672..17487
                     /gene="yedP"
                     /locus_tag="ACHKFK_003092"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO9053373.1"
                     /translation="MLSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAN"
     gene            complement(17484..19178)
                     /gene="dgcQ"
                     /locus_tag="ACHKFK_003093"
     CDS             complement(17484..19178)
                     /gene="dgcQ"
                     /locus_tag="ACHKFK_003093"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO9053374.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSGALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(19349..19531)
                     /gene="yodC"
                     /locus_tag="ACHKFK_003094"
     CDS             complement(19349..19531)
                     /gene="yodC"
                     /locus_tag="ACHKFK_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO9053375.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(19610..20527)
                     /gene="yedI"
                     /locus_tag="ACHKFK_003095"
     CDS             complement(19610..20527)
                     /gene="yedI"
                     /locus_tag="ACHKFK_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO9053376.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            20700..21620
                     /gene="yedA"
                     /locus_tag="ACHKFK_003096"
     CDS             20700..21620
                     /gene="yedA"
                     /locus_tag="ACHKFK_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO9053377.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFFAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(21609..22079)
                     /gene="vsr"
                     /locus_tag="ACHKFK_003097"
     CDS             complement(21609..22079)
                     /gene="vsr"
                     /locus_tag="ACHKFK_003097"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO9053378.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     DLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(22060..23478)
                     /gene="dcm"
                     /locus_tag="ACHKFK_003098"
     CDS             complement(22060..23478)
                     /gene="dcm"
                     /locus_tag="ACHKFK_003098"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO9053379.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSVWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISKCFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(23545..24240)
                     /gene="yedJ"
                     /locus_tag="ACHKFK_003099"
     CDS             complement(23545..24240)
                     /gene="yedJ"
                     /locus_tag="ACHKFK_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO9053380.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKDHPQRQRSSILAAEETRRLLREEFVQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(24280..24561)
                     /gene="drpB"
                     /locus_tag="ACHKFK_003100"
     CDS             complement(24280..24561)
                     /gene="drpB"
                     /locus_tag="ACHKFK_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO9053381.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         6138 a         6363 c         6146 g         5989 t
ORIGIN      
        1 cattgattag atgaatattt atcgcgcagt gacatcattt tttaataata gttcaaaaaa
       61 aagggcgtac aatgaaaaaa ttaacagtgg caatttctgc tgtagctgca tcagtactga
      121 tggcgatgtc tgctcaggca gcagagattt acaataaaga cagtaacaag ctggatctgt
      181 acggaaaagt taatgctaag cactacttct cttctaacga tgcagatgat ggcgacacaa
      241 cttatgttcg tctgggcttc aaaggcgaaa ctcaaatcaa cgatcaactg actggtttcg
      301 gtcagtggga atatgaattc aaaggcaacc gtgctgaatc tcaaggttct tccaaagata
      361 aaacccgtct tgccttcgct ggcctgaaat tcggtgacta cggctccatc gattatggcc
      421 gtaactacgg tgtagcatac gatatcggtg catggaccga cgttctgcca gaattcggtg
      481 gcgatacctg gacccaaaca gatgtgttca tgactggtcg caccacaggt gttgcaacct
      541 atcgtaacaa cgacttcttt ggtctggttg atggtctgaa ctttgctgct cagtatcagg
      601 gtaaaaatga ccgcactgac gtaactgaag ctaacggtga tggtttcggt ttctccacta
      661 cttatgagta tgaaggattc ggtgtaggtg caacctatgc taaatctgac cgcactaatg
      721 atcaggttat ctacggtaac aacagcctga atgcatctgg tcaaaatgct gaagtatggg
      781 cagctggtct gaaatatgat gcgaacaaca tctatctggc taccacctat tctgaaaccc
      841 agaacatgac tgtttttggt aataaccata ttgccaacaa agcacaaaac ttcgaagtag
      901 ttgcacaata tcagttcgac ttcggtctgc gtccgtccgt tgcttacctg caatctaaag
      961 gaaaagactt gggtgcgtgg ggtgatcagg acctggttga atatattgat gtaggtgcaa
     1021 cctattactt caacaaaaat atgtccactt ttgttgatta caaaatcaac ctgattgata
     1081 agagcgattt cacgaaagca tctggcgttg ctaccgatga tatcgttgct gtaggattgg
     1141 tttaccagtt ctaatctgat tactcaaaga tatgttgtgg gaggctttgc ctccccaaca
     1201 tataagtggc cccctcaagc cactttcctt agaagcacta tcttgcttct gactatataa
     1261 accttctgtt atatattacc ctttattttc tggggcgttt caacgcccca tttttaataa
     1321 tacccagtga acaattaacg tatcaactag atttaataac aacgatatcc gtacctaacc
     1381 tgatatctaa tgtcataagt atccctttaa ttatcccctc aacctcttgt aactttccca
     1441 ctcttgagct ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa
     1501 attgtgatgg aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca
     1561 gaagtcaccg gattgttcag gctccaatag gttgagtatg tcgtatttat tttccaaaat
     1621 aaaaatcgat gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc
     1681 tctcttggtg ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg
     1741 taaccatttt ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac
     1801 gcattattta acctcacgtc gtccaatata ctttgaaaac ttattccctc gcttgccaga
     1861 cggcgatgca atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat
     1921 tttgttccag gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta
     1981 tctgataaaa gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct
     2041 aagtgttcct gaatttgcgt tgagaagccg cggaaaatag atgtttttag ttgctcccaa
     2101 caatatatta actcggggtt ctgaggtaag cagaaacctg tcgtgttacg aatttgatca
     2161 gttatcgcat aaaatttttg aaacttttca attaaatcaa tgggataata aagcatttct
     2221 gcaagataaa gacctgcttc tggataattc gcaatataaa attcatatcc acagggaaat
     2281 aatattatct gattgttatc aacagttaga ctatgcgtct cccaattgat aacttttttt
     2341 ccctgacgga tacgacataa agctggcata agaggcttaa ccctgtgaat ctcatgatgt
     2401 ttatgcatcc gtatttcttc aatctttagg ttagacttat cttttgccag catactctta
     2461 ccctacttta tctcataaac tggtgttatc tcagcggttg caattttatt ggcattaagc
     2521 atataaccaa ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga
     2581 gcccatactt taaactggta atgatggggt ttatctcttt tgggaggaca tgctccacca
     2641 aatcctgcat aaccaaaatc atttcggcct tgaacagcac cagtcggaag ttttgtacca
     2701 tcacgtcttc ctgcatcagt gggcaaatat gttacagttg ctggaatatt agcaacagtc
     2761 cagtgccacc atccactgcc tgtaggagca tctggatcat acacagttac ggcaaagctt
     2821 ttggtgcctt caggagcacc agaccaggtt aatgagggcg atgtattacc cccttcacac
     2881 ccaaatccag aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta
     2941 gtgacctgaa atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg
     3001 ataagttttt tcataaaaac ctcattgttg tgacctatcg ttattttatt tgatgtgcct
     3061 tgatctcatt atgcataaag gcgcaatgat cgcgcaaatg caatcataat tataaaggtg
     3121 ccccgaacat tagccataat atgcacaaat cagattcctg ttatctcaag gataattatt
     3181 ttttaatgcg gtgcacttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac
     3241 atcatgccta tgatagctgg caggtccagt cgttgaccaa aaacccccca ggaaagcaaa
     3301 ctaatcagaa cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa
     3361 gccagcgtct gagctaataa ccagaatgat gcacaatagc aaataattgt gccaacagag
     3421 ggccataacc gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca
     3481 agtattgcgc caccaagata aatataaggg ttcatagcat attctttcct gttcatactg
     3541 gagagaattg tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc
     3601 cagggggctc tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc
     3661 aggctaacga atgagttcac tcttcctggg agcaaagcga agacaaaaca tgttatcgcg
     3721 tttacggatc gtggatggca gaaaacaacc aggatcaggt atccatttta aaccagaaat
     3781 tgagtgagtt tgccccatcc atgccccacg ctgtgggatc ggatataatt aaacaagcat
     3841 agtcaacagg ttacacaacc tacacctgca tggacatcac ttcctgatac gccgccacca
     3901 gcttattacg cacctgaatc cccatttgca tagaaactga ggctttttgc atatcggtca
     3961 tcacatcgtt tagcgccacg ccgggttcac cgagggtgaa tttttctgcc tgcgtgcggg
     4021 cgactgtttg tgtatcgctt atgcgatcga gcgcggcgtg cagctgcccg gcaaaactaa
     4081 tggtcggttg cggcagtgat tcctgcgcac gcgcactcat cgccgtagcc tgtaactggc
     4141 tgataacccc ttcaatcccc tgtatcgctg acattttcgt ctcccggata atttctggta
     4201 gcaaagccta ccagtaagtc aataagacaa aggcgctaaa tagcaacaaa aaaacgggtt
     4261 tattggcgga tagaaaaaaa cgaaagcaca aataatggga gcgtcaattt ttcgagtttg
     4321 ctgacccggg agtgagtctt gttccacttt gccaataacg ccgtccataa tcagccacga
     4381 ggtgcgcgat gaatgcgact gcagcccaga caaaatctct tgagtggctt aatcgcctgc
     4441 gtgcgaatcc gaaaattcca ttgattgttg ccggttccgc agcagtggcg gtcatggtcg
     4501 cactgatcct gtgggcgaaa gcccccgact accgcacatt attcagcaat ctttccgatc
     4561 aggatggtgg cgcaattgtc agccaactga cgcaaatgaa tattccttac cgcttcagcg
     4621 aagccagcgg cgctattgag gttccggcag ataaagttca cgaactgcgc ctgcgcctgg
     4681 cgcaacaagg tttgcccaaa ggcggcgcgg tcggtttcga actgctcgat caggaaaagt
     4741 ttggtatcag ccagttcagc gaacaggtga attatcagcg ggcgctggaa ggcgagcttt
     4801 ctcgtaccat cgaaactatc ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga
     4861 aaccgtcttt attcgtccgt gaacaaaaat ccccttctgc atcggtgacg gtaaacctgt
     4921 tacccggccg cgcactcgat gaagggcaaa ttagcgctat tgtacatctg gtttccagcg
     4981 cggttgctgg tctgccgcca ggaaacgtca cgctggtgga tcagggcggg catctgttaa
     5041 cccagtccaa taccagcggg cgcgatctta atgacgctca gttgaaatat gccagcgatg
     5101 tcgaaggccg tattcagcgg cgtattgaag cgatcctgtc gcctattgtt ggtaacggta
     5161 atattcacgc ccaggtcacg gcgcaactgg acttcgccag taaagaacaa acggaagaac
     5221 agtatcgccc taacggtgat gaatctcatg cggcgcttcg ttcacgccag cttaatgaga
     5281 gcgagcaaag cggttccggt tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg
     5341 cacctgcgaa taacgcgcca atcagcacgc ctccgacaaa tcaaaataac cgccagcagc
     5401 aggcgagcac caccagcaat agtgggccgc gtagcacaca gcggaatgaa accagtaact
     5461 acgaagtcga tcgcaccatt cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt
     5521 cagtcgcggt agtggtgaat tacaaaacct tgccagacgg taaaccattg cctctcagca
     5581 acgaacagat gaagcaaatt gaagctctga cccgcgaggc gatgggcttt tctgaaaaac
     5641 gcggtgattc gctcaatgtt gttaactcgc cgttcaatag cagtgacgaa agcggcggag
     5701 cactgccgtt ctggcaacag caagtgttta tcgatcagtt gctggctgcc ggtcgctggt
     5761 tgctggtact gctggttgcg tggttgctgt ggcggaaagc ggtacgcccg cagctaacac
     5821 gtcgcgccga ggcggtgaag actgtgcagc aacaggcgca ggcccgcgag gaagtggaag
     5881 atgcggtgga agtccgtctg agcaaagacg aacaactcca acaacggcgc gctaaccaac
     5941 gactgggggc agaagtcatg agccagcgta tccgtgaaat gtctgataac gatccgcgcg
     6001 tggtggcgct ggtcattcgc cagtggataa ataacgatca tgagtaacct gacaggcacc
     6061 gataaaagcg tcatcctgct gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag
     6121 cacctctccc agcgcgaagt gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc
     6181 tccaacaagc agctaaccga tgtgctggca gagtttgagc aagaagctga acagtttgcc
     6241 gcactgaata tcaacgccaa cgattatctg cgttcggtat tggtcaaagc tctgggtgaa
     6301 gaacgtgccg ccagcctgct ggaagatatt ctcgaaaccc gcgataccgc cagcggtatt
     6361 gaaacgctca actttatgga gccgcagagc gccgccgatc tgattcgcga tgagcatccg
     6421 caaattatcg ccaccattct ggtgcatctg aagcgcgccc aggccgccga tattctggcg
     6481 ttgttcgatg aacgtctgcg ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg
     6541 cagccagccg cgctggccga actgaccgaa gtactgaatg gcttgctcga cggtcagaat
     6601 ctcaagcgca gcaaaatggg cggcgtgaga acggcagccg aaattatcaa cctgatgaaa
     6661 acgcagcagg aagaagccgt tattaccgcc gtgcgtgaat tcgacggcga gctggcgcag
     6721 aaaatcatcg acgagatgtt cctgttcgag aatctggtgg atgtcgacga tcgcagcatt
     6781 cagcgactgt tgcaggaagt ggattccgaa tcgctgttga tcgcgctgaa aggggccgag
     6841 cagccactgc gcgagaaatt cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc
     6901 gacgatctcg ccaaccgtgg tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg
     6961 attctgctga ttgtgcgccg ccttgccgaa actggcgaga tggtgattgg cagcggcgag
     7021 gatacctatg tctgataatc tgccgtggaa aacctggatg ccggacgatc tcgcgccacc
     7081 acaggcggag tttgtgccaa tggtcgagcc ggaagaaacc atcattgaag aggccgaacc
     7141 cagccttgag cagcaactgg cgcaactgca aatgcaggcc cacgagcaag gttatcaggc
     7201 ggggattgcc gaaggtcgcc agcaaggtca tgagcagggc tatcaggaag gcctggccca
     7261 ggggctggaa caaggtctgg cagaggcgaa ggctcaacaa gcgccaattc atgcccggat
     7321 gcagcaactg gtcagcgaat ttcaaactac ccttgatgcg cttgatagtg tgatagcttc
     7381 gcgcctgatg cagatggcgc tggaggcagc acgtcaggtg atcggtcaga caccaacggt
     7441 ggataactcg gcactgatca aacagatcca acagttgttg cagcaagaac cgttattcag
     7501 cgggaaacca cagctgcgcg tacacccgga tgatctgcaa cgtgtggatg atatgctcgg
     7561 cgcaacttta agtttgcatg gctggcgctt gcggggcgat cccaccctcc atcctggcgg
     7621 ctgtaaagtc tccgccgatg aaggcgatct cgacgccagt gtcgccactc gctggcaaga
     7681 actctgccgt ctggcagcac caggagtggt gtaatgacca cgcgcctgac tcgctggcta
     7741 accacgctgg ataactttga agccaaaatg gcgcagttgc ctgcggtacg tcgctacggg
     7801 cgattaaccc gcgctaccgg gctggtgctg gaagccaccg gattacaatt gccgctcggc
     7861 gcaacctgtg tcattgagcg ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc
     7921 gttggcttta acggtcaacg gctgttttta atgccgctgg aggaagtcga aggtgtcctg
     7981 cccggcgcgc gtgtttatgc caaaaacatt tcggcagaag ggctgcaaag cggcaagcag
     8041 ttgccgctcg gtccggcgtt attaggtcgc gttctggacg gcagcggtaa accgcttgat
     8101 ggcctcccct cccccgatac gacggaaacc ggtgcgctga ttaccccgcc atttaacccg
     8161 ttgcaacgta caccgattga acatgtgctg gacaccggcg tgcgcccaat caatgccctg
     8221 cttaccgtcg ggcgcgggca gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt
     8281 gtgctgctgg ggatgatggc acgttacacc cgcgccgatg tcattgtcgt gggtttgatt
     8341 ggtgaacgag ggcgcgaagt aaaagatttt attgagaata tcctcggtgc cgaaggacgt
     8401 gcacgctccg tggtgattgc cgctccggcg gatgtttctc cgctcctgcg aatgcagggt
     8461 gccgcctatg ccacgcgcat tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg
     8521 attatggact ccctcacccg ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc
     8581 gaaccacctg ccactaaagg ttatccaccg tcggtgtttg ccaaattacc ggcactggtc
     8641 gagcgtgccg gaaatggcat tagcggcggc ggctcgatta ccgcgtttta taccgtgctc
     8701 accgaaggcg atgaccagca ggatcccatt gctgactccg cgcgggcaat cctcgacggt
     8761 cacattgtgc tgtctcgccg actggcggaa gccgggcact atccggctat cgatattgaa
     8821 gcgtcgatca gtcgcgcaat gacggcgttg atcagtgagc aacattacgc gcgagtgcgc
     8881 accttcaaac agctgttgtc gagttttcag cgtaaccgcg atctggttag cgtcggcgcg
     8941 tatgccaaag gcagcgatcc gatgctcgat aaagccatcg ccctgtggcc gcagctggag
     9001 ggctatttgc aacaaggcat ttttgaacgc gcggactggg aagcgtctct ccaggggctg
     9061 gagcgtattt tcccgacagt gtcataaccc aggagataac ggcagatggc ggaacatggt
     9121 gcgctggcga ccctgaaaga tctggcagaa aaagaggtag aggatgccgc gcgcctgctg
     9181 ggtgaaatgc gtcggggatg tcagcaggcg gaagaacagc tcaaaatgct gatcgattat
     9241 cagaatgaat atcgcaataa cctcaatagc gatatgagtg ccgggatgac cagcaaccgc
     9301 tggatcaact atcagcagtt tatccagacg ctggaaaaag ccattactca gcatcgccag
     9361 caacttaatc agtggacgca gaaagttgac attgccctga acagctggcg agaaaagaaa
     9421 caacgtttgc aggcctggca gacactgcag gaacggcaat ccacggcggc actgcttgca
     9481 gaaaaccgcc tcgatcagaa aaagatggat gagttcgccc agcgcgccgc catgaggaaa
     9541 cctgaatgat tcgcatagcg cccttgatta ccgctgacgt tgataacgcc acattgcctg
     9601 gcggcaaagc cagcgatgct gcacaagatt ttctcgcgtt gttgagcgaa gcattaacag
     9661 gcgaggcaac aaccgacaaa gcggcccctc agttgctggt ggcaacagat aagcccacgg
     9721 caaaaggcga gccgctggtc agcgatattg tttccgacgc gcaacaagca gatttactga
     9781 tccctgtgga tgaaacaccg cctgtcatca acgacgaaca atccacatca acaccgttaa
     9841 ccaccgctca gacgataacg ttggctgcgg cggctgacaa caatacggca aaagacgaaa
     9901 aagcggatga tctgaatgaa gacgtcaccg caagcctgag cgcccttttt gcgatgttgc
     9961 cgggttttga caatacgccc aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga
    10021 aaccaacggt cttcacaaaa ctgacttctg cgcaactcac tacagcacag cctgatgacg
    10081 cccccggcac accagctaag ccattaacac cgctggtagc agaagcccag agtaaagcgg
    10141 aagtcatcag cacgccttcg tcggtgaccg ctgccgccag cccgctaatc actccacacc
    10201 agacacagcc actgcccacc gtcgccgcgc ctgttttgag tgcaccgctg ggttctcacg
    10261 aatggcaaca atcattaagc cagcatattt cgctgttcac ccgccagggg caacaaagtg
    10321 cagagttgcg tctgcaccca caagatttag gtgaagtgca aatttccctc aaagtggatg
    10381 ataaccaggc gcaaatccag atggtttcac cgcatcagca cgtacgcgcc gccctggaag
    10441 cagcgctgcc ggtactgcgt acgcagctgg ccgaaagtgg cattcagtta gggcaaagca
    10501 acatcagtgg cgaaagcttt agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc
    10561 aacgcacagc aaaccatgaa cctctggcgg gggaagacga cgatacgctt ccggttcccg
    10621 tctctttaca agggcgtgta acaggcaaca gcggcgttga tattttcgcc taacgtcaga
    10681 ggtagcaccg taatccgcgt cttttccccg ctttgttgcg ctcaagacgc aggataatta
    10741 gccgataagc agtagcgaca caggaagacc gcaacacatg actgattacg cgataagcaa
    10801 gaaaagcaag cgatcgcttt ggatcccgat tctggtattc attaccctcg cggcctgtgc
    10861 cagcgcaggt tacagctact ggcattcgca tcaggttgcc gctgacgaca aagcgcagca
    10921 acgcgtcgtg ccctccccgg tcttctatgc gctggatacc tttacggtca atctgggcga
    10981 tgcggatcgc gtgctttata tcggcataac cctgcgactg aaagatgaag caacccgctc
    11041 gcggctgagt gagtatttgc cggaagtccg tagtcgcttg ctgttactgt tttcgcgtca
    11101 ggatgctgcc gtactggcga cagaagaagg caagaaaaac ctgattgccg agattaaaac
    11161 cacactttcc accccgcttg ttgccgggca accgaaacag gatgtcaccg acgtgctgta
    11221 taccgctttt attctgcgat aacgacatgg gcgatagtat tctttctcaa gctgaaattg
    11281 atgcgctgtt gaatggtgac agcgaagtca aagacgaacc gacagccagt gttagcggcg
    11341 aaagtgacat tcgtccgtac gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc
    11401 aggcgctgga aatcattaat gagcgctttg cccgccattt tcgtatgggg ctgttcaacc
    11461 tgctgcgccg tagcccggat attaccgtcg gggccatccg cattcagccg taccatgaat
    11521 ttgcccgcaa cctgccggtg ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg
    11581 gcacagggct ggtggtgttc tcaccgagtc tggtgtttat cgctgtggat aacctgtttg
    11641 gcggcgatgg acgcttcccg accaaagtgg aaggccgcga gtttacccat acagaacagc
    11701 gcgtcatcaa tcgcatgtta aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga
    11761 ttaatccgct ggaagttgag tacgtgcgtt cggaaatgca ggtgaaattt accaatatca
    11821 ccacctcgcc gaacgacatt gtggttaaca cgccgttcca tgtggagatt ggcaacctga
    11881 ccggcgaatt taatatctgc ctgccattca gcatgatcga gccgctacgg gaattgttgg
    11941 ttaacccgcc gctggaaaac tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc
    12001 gccaggtgca gcattcacag ctggaactgg tggccaactt tgccgatatc tcgctacgcc
    12061 tgtcgcagat tttaaaactg aaacccggcg acgtcctgcc gatagaaaaa cccgatcgca
    12121 tcatcgccca tgttgacggc gtcccggtgc tgaccagtca gtacggcacc ctcaacggtc
    12181 agtatgcgtt acggatagaa catttgatta acccgatttt aaattctctg aacgaggaac
    12241 agcccaaatg agtgacatga ataatccggc cgatgacaac aacggcgcaa tggacgatct
    12301 gtgggctgaa gcgttgagcg aacaaaaatc aaccagtagc aaaagcgctg ccgacgcggt
    12361 gttccagcaa tttggcggtg gtgatgtcag cggaacgttg caggatatcg acctgattat
    12421 ggatattccg gtcaagctga ccgtcgagct gggccgcaca cgaatgacca tcaaagagtt
    12481 attgcgtctg acgcaaggat cagtcgtggc gctggacggt ctggcgggcg aaccgctgga
    12541 tattctgatc aacggttatt taattgctca gggcgaagtt gtcgtagtcg ctgataaata
    12601 tggcgtgcgg atcaccgata tcattactcc gtctgagcgg atgcgccgcc tgagccgtta
    12661 gtgatgaata accacgctac tgtgcaatct tccgcgccgg tttctgctgc gccactgctg
    12721 caggtgagcg gcgcactcat cgccattatt gccctgatcc tcgctgctgc ctggctggta
    12781 aaacggctgg gatttgcccc taaacgcact ggcgttaacg gtctgaaaat tagcgccagt
    12841 gcttcactgg gcgcgcgtga acgggttgtg gtggtcgatg tggaagatgc acggctggtg
    12901 ctcggcgtta ccgcagggca aatcaatctg ctgcataaac ttcccccttc agcaccaacg
    12961 gaagagatac cgcagaccga ttttcagtcg gtcatgaaaa acttgcttaa gcgtagcggg
    13021 agatcctgat gcgtcgtttg ttgtctgtcg cacctgtcct tctctggctg gttacgcccc
    13081 tcgccttcgc gcaactgccg ggtatcaaca gccagccgct gcctggcggt ggacaaagct
    13141 ggtcgctccc ggtgcaaacg ctggtgttca tcacctcgtt gacgtttatt ccggcaattt
    13201 tactgatgat gaccagtttc acccgcatca tcattgtttt tggtttattg cgtaacgcgt
    13261 tgggaacacc ctccgcgcca cctaaccagg tattgctggg actggcgctg tttttgacct
    13321 tttttattat gtcgccggtg atcgataaaa tttatgtcga tgcgtaccag ccattcagcg
    13381 aagagaaaat atcaatgcag gaggcgctgg aaaaaggggc gcagccgctg cgtgagttta
    13441 tgctgcgtca gacccgtgag gcggatttag ggttgtttgc cagactggcg aatactggcc
    13501 cgttgcaggg acctgaagct gtgccgatgc gcattttgct cccggcctac gtgaccagcg
    13561 agttgaaaac cgcatttcag ataggcttca cgattttcat tccttttttg attatcgacc
    13621 tggtgattgc cagcgtgttg atggcattgg ggatgatgat ggttccccca gccacaattg
    13681 ctctgccctt taaactgatg ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt
    13741 cgctggcgca gagcttttac agctagtgag gcaaaatgac acctgaatcg gtcatgatga
    13801 tggggactga agcgatgaaa gtcgcgctgg cactggctgc cccactgttg ctggtggcgc
    13861 tggtcacggg tcttatcatc agtattttgc aggccgccac ccagattaac gaaatgacgc
    13921 tgtcgtttat tccgaaaatc atcgccgtat ttatcgccat tattattgcc ggaccgtgga
    13981 tgctcaatct gttgctggat tacgtccgca ccttgttcac taacctgccg tatatcatcg
    14041 ggtaggccgt acaatgatgc aggtaacaag cgatcaatgg ctttcctggt taagcctgta
    14101 cttctggccg ttactgcgcg tgctggcgct catttctacc gcaccaattc tgagcgaacg
    14161 cagcgtgccg aaacgggtaa aactaggtct ggcaatgatg atcactttcg ctattgcccc
    14221 atcattacct gccaacgatg ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca
    14281 gcagatcctg atcggcattg cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg
    14341 aaccgctggt gaaattatcg gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc
    14401 ggccagccat ctcaatatgc ccgttttagc gcgtatcatg gatatgctgg cgttactgct
    14461 gttcctgaca tttaacggtc atttatggtt gatttcactg ctggtcgata cctttcacac
    14521 cctgccaatt ggtggcgaac cgttgaacag caatgcgttt ctggcactca ccaaagcagg
    14581 gagtttgatt ttccttaacg ggctgatgct ggcattaccg ctcattactc tgctgctgac
    14641 cctgaatctg gcattaggct tacttaatcg tatggccccg caattatcca tttttgttat
    14701 tggatttcca ttaactctga ctgtcggtat ctctttaatg gcggcattaa tgccgttaat
    14761 tgcgcctttt tgcgaacatt tattcagtga gatgtttaat ttgctggctg atattattag
    14821 tgaattgcca ttaatataat tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa
    14881 aataatacct acgaacatct tccaggatac tcctgcggcg aaatatttgt tttaagctca
    14941 ctcacatatc gcaacattta ctttacttta agacaattcc aggcaaatta tacaacactt
    15001 tacgggatag taagtccgcc tgaaaaatcg cgagagtggc gcattaggtg acccatgttg
    15061 ttccgtttag tcatgatgaa atattcaggt aaggggaatt atcgttacgc attgagtgag
    15121 ggtatgccat gtcaacgatt attatgaatt tatgtagtta cacccgacta ggtttaaccg
    15181 ggtatctgtt gagtagaggg gttaaaaaaa gagaaatcaa cgacattgaa accgttgatg
    15241 accttgccat agcttgtgat tcacagcgcc cttcagtggt gtttattaat gaggactgtt
    15301 tcatccacga tgcttctaac agtcagcgta tcaagctcat cattaatcaa catcccaata
    15361 cgttatttat cgtttttatg gcaattgcca atgttcattt tgatgaatat ctattggtca
    15421 gaaaaaattt attgatcagt tctaaatcga ttaaaccgga atctctcgac gatatccttg
    15481 gcgatattct gaaaaaagag acaacgataa cctcgttttt aaatatgccg acgttatcat
    15541 tgagccgaac cgaatcgagt atgttgcgaa tgtggatggc aggtcaggga accattcaaa
    15601 tctctgacca aatgaatatc aaagccaaga ccgtttcatc gcataaaggt aatattaaac
    15661 gtaagatcaa aacgcataat aaacaggtta tctaccatgt cgtccgactg acggataatg
    15721 tgactaatgg tatttttgtc aacatgcgct aacacattct gactggtggt ttcccaccag
    15781 tcaggctgaa ttagattact ctgctttctc cacaaagata ccgtcctgat gccctgcttc
    15841 attaaagaac cagatgccga gcgggtagtc ttccagcgaa accaggtaca ttgtgccttc
    15901 actaaactcc tcaactgcca gtaccacgcc aggacgacgc ggaccgccat ccgttttgac
    15961 tgttacccga tcattcacct tcatcgtttt cctcctgtgg ctttgtgcca gtgtagaaca
    16021 atttcgtttt tctggcagcg ccaggcgcgc gtgagtgctg attttctcga cggtctatac
    16081 ttaagagatg ccaggcggac ttaacgactg gcggcaacaa cagagtaacg gttgcgagga
    16141 aagatgatga aaaccgcaaa agagtacagc gataccgcaa aacgtgaggt cagcgtcgat
    16201 gtcgatgccc tgctggcggc gatcaatgaa attagcgaaa gcgaagttca tcgcagccag
    16261 aacgattctg aacacgttag cgtcgatgga cgtgaatatc atacatggcg tgaattggcg
    16321 gatgccttcg aactggatat tcatgacttc agcgtctctg aagtgaatcg ttgaatgcac
    16381 aataaaaaaa tcccgaccct gagggggtcg ggatgaaact tgcttaagca agaagcactt
    16441 aaaaaattcg ttacaccagg aaatctgatg tgttcatcac cttatccgca atttttttcg
    16501 ctgacaagaa aatattcgtg atatgaatga ttaacgctac ttatgaatag aaatgtgacg
    16561 cgcgtcaccc cttggtggct ctggcgtcaa cgggatcgct cccgaaaaaa acatctttga
    16621 taatttgtgc tgttttgaga atcatcctgg taaggggagt actgcgcaac catgctttca
    16681 attcaacaac cactactggt ttttagcgat cttgatggca ccctgctgga cagtcatagt
    16741 tatgactggc aaccagctgc cccctggctc agccgtttac gcgaagcgaa tgttcccgtc
    16801 attctctgta gcagtaagac atcagcggaa atgctgtact tgcaaaaaac gttggggcta
    16861 caaggtttac cgctgattgc cgaaaatggc gcagtgatcc agcttgctga gcaatggcag
    16921 gatatagacg gttttccacg catcatctca ggtattagcc atggcgaaat cagccaggtt
    16981 ttaaatacgc tacgcgagaa agaacatttt aaattcacaa cttttgatga tgtcgacgat
    17041 gcaaccatcg ccgaatggac gggattaagc cgtagccagg cggcgctgac gcagctgcat
    17101 gaggcgtcgg taacgctaat ctggcgcgac agtgacgagc gtatggcaca atttaccgcg
    17161 cgtctgaacg aactgggctt gcagtttatg cagggtgcgc gcttctggca cgtcctggat
    17221 gcctctgccg gaaaagatca ggctgccaac tggattatcg cgacctatca acaattgtca
    17281 ggcaaacgcc caaccacact tggcctgggc gatgggccaa acgatgcgcc cttactggag
    17341 gtaatggatt acgcggtgat tgtgaaaggg ttaaaccgtg aaggggtgca tctgcatgat
    17401 gaggatccgg cccgcgtctg gcgaacgcag cgtgaaggac cggaaggctg gcgtgaaggg
    17461 ctggaccatt ttttctccgc caattaagcg ttatcgctcg cgaatacccg attgcgcccg
    17521 gcctgtttag cgagataaag tcgacggtcg gccagtgact ggagttgttc aaaatcataa
    17581 tcaccggttt cctcgctgct gcttaccccc agcgaggcac tgatgcgtat cgtcgtactc
    17641 ttggcgatca acatctcttt ttcatttagc ttcaggcgaa tacgttctgc aacctccgca
    17701 gcctgcgtca ggctcgcgcc aggcagaatc acacaaaact cctcaccacc gacccgcccg
    17761 gcaacgtcct gcgcacgcaa ggaactgcta attaatccgg cagcatgaga aagaacacgg
    17821 tcgcccgcct gatgaccaaa gcggtcatta atcgctttaa aatggtcaag gtcgacctga
    17881 atgacagaaa aaggatgttg gtgtgtctga cacaatttgg cgagcggacg ggctttttca
    17941 aacagtgcgc cacgattata taagcgggtt aaggtgtcgt gccacgcctg ccactgcaac
    18001 gaactttgca gaacatacat gttgctgacc atccggcgaa tcacatacca ggagatgagt
    18061 aacatggtgg taaagagcgc ccacagcagg gttaatgcaa tgctgatact gccgaaatcg
    18121 ccccgcacgc cttcgcttag cgtatggacg cgcaccagca caccgtcgaa atggtccaga
    18181 cgttcccagc taacatagcg actgttcata cgaatgccgc cccgcgtgtc atgttccatt
    18241 gcctgcgcca gcaaggccag ttcacgagga tcaaaaatat tccctgttgg atggtcagga
    18301 ttggaagagg tcaaaaatct cagcttactg tcatagagct gatactcacc atcgaggttt
    18361 ttatcgatgg cgtttcttaa aaattgctgc atggtacgca cgggaatact catccccagc
    18421 acgccatacc agtagttatt actgtctacc ggaacactga cggtaacctg cggttcagta
    18481 ttgctggcgt gttccggttg cgaagtgaac cagcgtaccg cgcggtgacg attttctcgt
    18541 tgtgaatggc cgataaacca gggttgggtg acatagccgt aataacgcgt tggtacattg
    18601 cgcgtaaata aggtcggctg cgtcgaaacg taaaatccgg cacgcgagac atacatcgcc
    18661 tgttcaacca tcgacgagga gttgtgcgcc agtcgcagca agtaaccaac ttccagtgca
    18721 gcggtaattt cattgtcgag gctttcattt tcgcgagaca ggagattccc ctcgctgact
    18781 aatgcacccg acacgccatt gactggcagg gtgcgtcgtc ggttgagttc gatttgccag
    18841 gcgtgctcgt cgcgatgctg ttcgaactcg gttacggcat tacgcagaga ggtgaaatcc
    18901 agtggcgcta cgagagcttc gcgcatgcca ttacgcaaaa agatcagttt gtcgacgtta
    18961 tactgcaaat gcttatcgag cgcgttggct acgttttcca gatgattacg ctggctggag
    19021 atataggcat cttccagcac taccacttcg cgccaggtca gcaaggtgga aaaaagcaat
    19081 accacgataa agcagagatt aaccacatga ccaggcccca ggcggcgtgc gagttttttc
    19141 aaccagctct ggttttccat ttttgtctcg tgctgcaccc tgacgactcc caacctgctt
    19201 ttttatgatt ctggcataac ccggcgataa cgtaccgcaa aacagcgata attttataat
    19261 ggtggcatga taagacgcga ctatcattca ttgcaggcat tggcacacca ggccggatgt
    19321 gatccggcct ggtgttaatg ataccctttc aaacttcttc cgcagaacga ctcccctccc
    19381 ccggaacaag ctcggtttca tgaaaagctt cccgcttgac cccgtaaccg tcataccaac
    19441 gacactcaac cataccgctg gagtatccag tgacaatcat ccgcgggccg ccctctttaa
    19501 ccgtaacttc ctcactaacc ataaagctca tactcgcctc cttttttcga gtgaaacttg
    19561 ttcaccttag ttgaagatgg cgaattttgc ataatagcct gtgcgagtat taatgtgcct
    19621 gaccgcgcat tttcgctacg gctttcactc ccagcaccac gatgccgccg atgataaatc
    19681 caagaatcag atttaaaaca gtcggtaata tcatcgccac cactgcactt tgctgcccgg
    19741 cgaaatgttc aatggcgtga tgcaacggcg caataccatg taccacaatc ccgccgccga
    19801 cgaggaacat cgccagcgtg ccgacaatcg ataatgcttt catcagccag ggcgcgataa
    19861 tcaataaccc tttacctaat gcctgcatca gcgcgctgga tttttccgcc agccaatacc
    19921 ccaggtcatc aatcttaacg ataaccccta ccagaccgta gacgcccacg gtcactacca
    19981 gcgcgatgcc tgaaagcacc agcacctgat taagcaacgg tgcctctgcc acaatcccca
    20041 gcgtgatggc gacgatttcc gcagacaata taaaatcggt acgaatcgcc ccttttattt
    20101 tgtctttttc aaacttcagc ggatcctgcg ccgccagctt ctccagacgc tgctgactct
    20161 gcgccggatc ttctttatgt ttacgcgcct ccagcatatg cagcactttc tctacccctt
    20221 caaagcagag aaacgcgcca ccaatcatca acagtggcgt aatcgcccac gggataaacg
    20281 cactgatgat cagcgccagc ggcaccagaa tcactttatt gatcagcgat cctttcgcca
    20341 cgccccagac cacgggaagt tcccggttgg cccgcacgcc tgaaacttgt tgcgcattga
    20401 gcgataagtc atcccctaat acaccggcgg ttttcttcgc cgccagtttg cccatcacgg
    20461 agatatcgtc cagcagtgtg gcgatatcat cgagcaacgt cagtaaacta ctcccggcca
    20521 aaagcattcc ctctcatttt tattgttgaa gggtgtaagt atgaagcaaa aacggcagct
    20581 ctgatacttg cttcacaggt caacatttat ttaacaatca agtgcaatta tatctctcgc
    20641 cagcagaatg attttcacgt ttactatgaa ccaccttttt atttccaccg tgagggatta
    20701 tgcgtttccg ccagttgtta ccgctttttg gcgcgctgtt tgcgttgtat atcatttggg
    20761 gctcaaccta ttttgtcatt cggattggcg tggaaagctg gcctccatta atgatggcgg
    20821 gcgttcgatt cctggcagcc ggtattttat tgctggcatt tttgctactg cgcggacaca
    20881 agctcccccc gctacgtccg ctgctcaatg ccgcgctgat tggcctgtta ttgctggctg
    20941 tcggtaatgg catggtgacg gttgccgaac atcaaaatgt tccttccggc atcgccgccg
    21001 ttgtggttgc aaccgtgccc ctctttaccc tgtgcttcag ccgcctgttt ggcattaaaa
    21061 cgcgcaaact ggaatgggtg ggtatcgcca ttgggcttgc cggaatcatc atgctcaata
    21121 gcggtggaaa tttaagcggc aatccgtggg gcgcgattct gattttaatc ggttcgatta
    21181 gctgggcgtt tggctcggtt tatggctcgc gcattacctt acctgtaggg atgatggcgg
    21241 gtgcgattga gatgctggca gcaggcgtgg tgttaatgat tgcgtcgatg attgcgggtg
    21301 aaaaactgac ggcgctccct tccctttcag gcttctttgc ggtcggctat ctggcgctgt
    21361 ttggttcgat tatcgctatt aacgcttata tgtatttaat ccgcaacgtc agtccggctc
    21421 tcgccaccag ctacgcctac gttaacccag tggtcgcggt cttgctgggt acgggactgg
    21481 gcggcgaaac gctgtcgaag attgaatggc tggcgctcgg cgtaattgtt ttcgcggtgg
    21541 tactggtcac gttgggaaaa tatctcttcc cggccaaacc cgttgtcgcg ccagttattc
    21601 aggatgcatc aagcgagtaa atgaatcccc tgcgtgtcga tctgcgcgct ggcaccttcg
    21661 ccgcagatcc actcttccag acgctcggta agcgccgcat ccgtcagctt ctcgcgccca
    21721 cgtaacgcac actcccagac aatcagtacg cgccagccga gttcctgcaa gcgactgata
    21781 tcgcggcgat cgcgttcaac atttttacct atcttctcca gccagaactc ggttcgagtc
    21841 gcaggcactt taaacagata gcagtgatga tgatgccaga agcagccgtg ggtaaatatc
    21901 acgcagcgat attcatcaac gacaaaatcc gggcgtccgg ggagatccgc gtcctgaacg
    21961 cgaaacgcca ggccctgccc ggttaacaaa ctggcgaggc gcttctcaat cgccgtatca
    22021 cgcgtagcaa tcgcgcgcat atttttgctg cgagtggcct tatcgtgaac gtcgaccatg
    22081 ttgtgcctct tgctgacgca acgccaccgc ctgtttgatt tttggctcaa gcagttttgc
    22141 caccgcggca aagaccggca cgaccaccga gttaccaaac tggcgataag cctgagtgtc
    22201 cgaaaccgga atgcggaatt tcgcttctcc cggcgcttca aaacccatta agcgcgcgca
    22261 ttcacgaggc gttaaccgac gtggacgatg ttgctgattc agcggatcgt caaagtcttt
    22321 ttcacccgtg gccatatccc agccgcgatc gattaaaatt tccgcgccat ctttgtaata
    22381 acgcgcagac aacgtacgcg tgacgctttg cggattgttc ggataaacca ttccataacc
    22441 gaagccatta ccacgcgcct gatgtttttt cgcataacga tagaggtact tccacagcac
    22501 cggcgtcagg atatatttcg cctcgaccat cgggtccagc agctgggcca gcgtcactcg
    22561 ctgcgcaggg aaacatttgc tgatatcacg cagggtaaaa tcggctttca gattcagatc
    22621 gcgacgaaaa cccaccagca cgatgcgttc acggtgctgc ggcaggaaat gtttgccatc
    22681 gatgattttc ggatcgtccg gcccgttatc ttctgcatca gccacgtcat agcccagttc
    22741 gtccagcgtc tgcatgatga tgcggaacgt tttaccctgg tcgtgacttt tcaggttttt
    22801 gacgttttcg agcacaaaca tcgccggacg acgcgcgtcg ataatgcgca ccacatcaaa
    22861 gaacagcgtg ccctgggtat cgcaggcaaa accgtgcgcc cgcccgagcg agttcttttt
    22921 cgatacgcca gccagcgaaa atggctgaca aggaaaaccc gccagtaata catcgtgttc
    22981 aggaatgtgt tgacgaatat gttccgccgc cgcctcatca ctcacgcctt ctttatggct
    23041 gagggtgatg tcgcggatat cttcattaaa atgatgcgtc gccgggtcgc aataatgatt
    23101 ggctttataa gtgcgtaccg catgtttgtt ccattcgctg gtaaacacgc actgtccgcc
    23161 aatcgattca aaaccgcgac ggatgccgcc aattccggca aatagatcga taaagcgaaa
    23221 cgcataatgc ggatgatgtg ccggtggttt gggtaacaac gtttgcagat gggcgaactc
    23281 tttctcactt aaacggtgcc ataccgagtc attcgccagc gcacgtttta aaattgccgc
    23341 gctccagtga ttctcaccca caccattaag ctgcgccacc agcgttttaa catcataaat
    23401 ttgcagcagt ttctccagca ttgcctgtgc ggcattcccg gtgctgtatg aatcggttac
    23461 tgatatattt tcctgcatag atttaaccgg ccatctgaga tggccgacag gttaacataa
    23521 ttacagccat ttagggccac gcgctcagcc cgcgggtgaa aacgcatcta tcaccttgtg
    23581 atcgacacct tcattctccc ccgccagttc ggcactgagc ttcgccataa actcgactaa
    23641 aaattgcgcg ttgtgctgcg ccagctgctt gcctcttgcg gtttgcatgg tttgcggtag
    23701 cttcagcaac ttagtctgga aatgatccag cgcgtagcgt ttatcatcaa gcggacgatg
    23761 ctgtgcgaac ggatcttcac catcaaacag cgccacgccc aatgcccctg aaacggcaaa
    23821 cacgcgcgcc aggccaatcg cccccagggc ttccagccga tccgcatcct ggacgatttt
    23881 agcttccgtt gttaaggggg cgatttgcgc gctgaaactg tgagcggcaa tggcatgaca
    23941 aacggcctcg attttctccg ccggaaattg cacgaactct tcacggagca ggcgacgcgt
    24001 ctcttctgcc gccaggattg aagaacgctg ccgttgtgga tgatctttcg ccaggctcac
    24061 aatatcgtga aaataacagg cggttaaaat caccagcata tcgacatcgt catctgccgc
    24121 cagcttctgt gccgtagccc agacgcggcg aaaatgacat acgtcatggg ccgcgtcctg
    24181 gtgctgatga tggtttttca accagttttc gaactgtgcc tgccagtgtt gtaagtccat
    24241 acgcgctccg ttgccaaaga gtcgcaacct tagcagtttt tattcatagc gtctgctacg
    24301 tgcgggacga ggtcgggtgt accaggcaat gctgcctaac aatgccccaa ccagagccat
    24361 aaataaaaag ccgaccaatg ctcctaacca cttccccgca gtagatccta aatcactttc
    24421 aaatcccgac acgggggcat cggggatccg gctcatcacc cagatgtaac gcgccattgc
    24481 ccagacgaaa tagccacacc atgtatagaa agcccacaaa gccagtttgc caccaggact
    24541 gcgagagagt ctctcttcca tctttgtcga accgtattcc attttgagat aacccggaaa
    24601 attcagactt agtactatta tgtgataaaa gtcaca
//
LOCUS       DAZGJT010000071        24001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZGJT010000071 DAZGJT010000000
VERSION     DAZGJT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.071"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..1472
                     /gene="galP"
                     /locus_tag="ACHKFK_003101"
     CDS             78..1472
                     /gene="galP"
                     /locus_tag="ACHKFK_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO9053382.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            1549..2046
                     /gene="yggI"
                     /locus_tag="ACHKFK_003102"
     CDS             1549..2046
                     /gene="yggI"
                     /locus_tag="ACHKFK_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO9053383.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            2141..2848
                     /gene="endA"
                     /locus_tag="ACHKFK_003103"
     CDS             2141..2848
                     /gene="endA"
                     /locus_tag="ACHKFK_003103"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO9053384.1"
                     /translation="MYRYLSIAAVVLSTAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     VAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWDKMYPVTDRECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            2928..3659
                     /gene="rsmE"
                     /locus_tag="ACHKFK_003104"
     CDS             2928..3659
                     /gene="rsmE"
                     /locus_tag="ACHKFK_003104"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO9053385.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            3672..4622
                     /gene="gshB"
                     /locus_tag="ACHKFK_003105"
     CDS             3672..4622
                     /gene="gshB"
                     /locus_tag="ACHKFK_003105"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO9053386.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMADLY
                     LINGEARARTRTLSVEQNYDKWYEFTGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEDKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            4731..5294
                     /gene="yqgE"
                     /locus_tag="ACHKFK_003106"
     CDS             4731..5294
                     /gene="yqgE"
                     /locus_tag="ACHKFK_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO9053387.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            5294..5710
                     /gene="ruvX"
                     /locus_tag="ACHKFK_003107"
     CDS             5294..5710
                     /gene="ruvX"
                     /locus_tag="ACHKFK_003107"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO9053388.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNI
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRVHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(5884..6864)
                     /gene="yggR"
                     /locus_tag="ACHKFK_003108"
     CDS             complement(5884..6864)
                     /gene="yggR"
                     /locus_tag="ACHKFK_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO9053389.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYTSQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQIGQQVGMITFQQSYQQRVKEGRL"
     gene            6882..7586
                     /gene="yggS"
                     /locus_tag="ACHKFK_003109"
     CDS             6882..7586
                     /gene="yggS"
                     /locus_tag="ACHKFK_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO9053390.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGITGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            7604..8170
                     /gene="yggT"
                     /locus_tag="ACHKFK_003110"
     CDS             7604..8170
                     /gene="yggT"
                     /locus_tag="ACHKFK_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO9053391.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFVKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            8167..8457
                     /gene="yggU"
                     /locus_tag="ACHKFK_003111"
     CDS             8167..8457
                     /gene="yggU"
                     /locus_tag="ACHKFK_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO9053392.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            8465..9058
                     /gene="rdgB"
                     /locus_tag="ACHKFK_003112"
     CDS             8465..9058
                     /gene="rdgB"
                     /locus_tag="ACHKFK_003112"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO9053393.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETMKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            9051..10187
                     /gene="hemW"
                     /locus_tag="ACHKFK_003113"
     CDS             9051..10187
                     /gene="hemW"
                     /locus_tag="ACHKFK_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO9053394.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAANLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEPAPRVEFSQYTGLS
                     EEVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10252..11259)
                     /gene="yggM"
                     /locus_tag="ACHKFK_003114"
     CDS             complement(10252..11259)
                     /gene="yggM"
                     /locus_tag="ACHKFK_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO9053395.1"
                     /translation="MKKQWIVGTALLMLMTGNVRADGEPPTENILKDQFKKQYHGILK
                     LDVITLKNLDAKGNQATWSVEGDVSSSDDLYNWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     NQNNSIPALKKDVKALDKQMVAAQKAADAYWGKDANGKQRTREDAFKKIHQQRDDFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWITLEKGQYPLTMKVSEINSKKVTILMKIDDINQVNERWKKDTEQLRRNGVI
                     K"
     gene            complement(11376..12422)
                     /gene="ansB"
                     /locus_tag="ACHKFK_003115"
     CDS             complement(11376..12422)
                     /gene="ansB"
                     /locus_tag="ACHKFK_003115"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO9053396.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDDVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKTVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(12598..13317)
                     /gene="yggN"
                     /locus_tag="ACHKFK_003116"
     CDS             complement(12598..13317)
                     /gene="yggN"
                     /locus_tag="ACHKFK_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO9053397.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(13501..13827)
                     /gene="yggL"
                     /locus_tag="ACHKFK_003117"
     CDS             complement(13501..13827)
                     /gene="yggL"
                     /locus_tag="ACHKFK_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO9053398.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLEEVRTSEL
                     FDVWWD"
     gene            complement(13827..14546)
                     /gene="trmB"
                     /locus_tag="ACHKFK_003118"
     CDS             complement(13827..14546)
                     /gene="trmB"
                     /locus_tag="ACHKFK_003118"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO9053399.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGIFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            14707..15759
                     /gene="mutY"
                     /locus_tag="ACHKFK_003119"
     CDS             14707..15759
                     /gene="mutY"
                     /locus_tag="ACHKFK_003119"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO9053400.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMVRFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVAALHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEDNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            15787..16062
                     /gene="yggX"
                     /locus_tag="ACHKFK_003120"
     CDS             15787..16062
                     /gene="yggX"
                     /locus_tag="ACHKFK_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO9053401.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            16127..17206
                     /gene="mltC"
                     /locus_tag="ACHKFK_003121"
     CDS             16127..17206
                     /gene="mltC"
                     /locus_tag="ACHKFK_003121"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO9053402.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQIES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            17408..18664
                     /gene="nupG"
                     /locus_tag="ACHKFK_003122"
     CDS             17408..18664
                     /gene="nupG"
                     /locus_tag="ACHKFK_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO9053403.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAEVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(18713..20848)
                     /gene="speC"
                     /locus_tag="ACHKFK_003123"
     CDS             complement(18713..20848)
                     /gene="speC"
                     /locus_tag="ACHKFK_003123"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO9053404.1"
                     /translation="MKSMNIAASSELVSRLSTHRCVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENIFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGANWHGFEGYAADQYFVDPCKLLLTTPGIDAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(21011..21094)
                     /locus_tag="ACHKFK_003124"
     CDS             complement(21011..21094)
                     /locus_tag="ACHKFK_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053405.1"
                     /translation="MHATSLHSLAYRRFARNSSLFMLLLMR"
     gene            21247..21954
                     /gene="yqgA"
                     /locus_tag="ACHKFK_003125"
     CDS             21247..21954
                     /gene="yqgA"
                     /locus_tag="ACHKFK_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO9053406.1"
                     /translation="MVIGPFINASAVLLGGVFGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTVVAKAHNLFHHSRKKPAHESF
                     IQNFVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            22060..22135
                     /locus_tag="ACHKFK_003126"
     tRNA            22060..22135
                     /locus_tag="ACHKFK_003126"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22093..22095,aa:Phe,seq:gaa)
     gene            complement(22334..23329)
                     /locus_tag="ACHKFK_003127"
     CDS             complement(22334..23329)
                     /locus_tag="ACHKFK_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9053407.1"
                     /translation="MKLSHADMRWNELMDEYFFCRSVRVATEWSYRKVLNGFRKFVGE
                     TLLPEDIRQQHVREWRREVLKKQNRSTHTWNNKVRHMRAIFNFACSSTLLNLSENPFD
                     GMNVRQEKKRKKTLTKKQVKRILLILEQFHEEEKRSDGVHWRSCAIRPAWYWMIVVNT
                     LRFTGMRLNQLLHLRLRDVNLDERWIDLCSEGSKTHHEWRIPMVSHLVPGMRHLIDEA
                     MKRGAGPEDYLFHYHRYVALMSEAPFVTGLPELQPVKGFFRRLTKECGFPVSPHRFRH
                     TLASTLMSAPERNLYMVKTMLGHRNVSTTLEYVEIDLKGTARVLEKELAELTDVF"
     gene            complement(23360..>24001)
                     /locus_tag="ACHKFK_003128"
     CDS             complement(23360..>24001)
                     /locus_tag="ACHKFK_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HFO9053408.1"
                     /translation="EDDAGCETESIPTDEDTAMLVSMFAEEARAPEKGEDDSPSGEVV
                     APVSSTGEISAAGHGEICQSYQDAEQQEYCEAESDAVNPDLTGERFLEWLKDGLKNGS
                     LSFNNKGDKIHVVAGFVFIPVPGIFYDYLKARQLPGEKREVLQTAFERLNIHKRKDNQ
                     RFYHARIAEAPGKTHGYKILKGYLIKGRGLMDTGDLNDSPNFFFSERRRSVFW"
BASE COUNT         5743 a         6328 c         5940 g         5990 t
ORIGIN      
        1 tgatttgctt cacatctttt tacgtcgtac tcacctatct taattcacaa taaaaaataa
       61 ccatattgga gggcatcatg cctgacgcta aaaaacaggg gcggtcaaac aaggcaatga
      121 cgtttttcgt ctgcttcctt gccgctctgg cgggattact ctttggcctg gatatcggtg
      181 taattgctgg cgcactgccg tttattgcag atgaattcca gattacttcg cacacgcaag
      241 aatgggtcgt aagctccatg atgttcggtg cggcagtcgg tgcggtgggc agcggctggc
      301 tctcctttaa actcgggcgc aaaaagagcc tgatgatcgg cgcaatcctg tttgttgccg
      361 gttcactgtt ctctgcggct gcgccaaacg ttgaagtact gattctttcc cgcgttctgc
      421 tggggctggc ggtgggtgtg gcctcttata ccgcaccact gtacctgtca gaaattgcgc
      481 cggaaaaaat tcgcggcagt atgatctcga tgtatcagtt gatgatcact atcgggatcc
      541 tcggcgctta tctttctgat accgccttca gctacaccgg tgcatggcgc tggatgctgg
      601 gtgtgattat catcccggca attttgctgc tgattggtgt cttcttcctg ccagacagcc
      661 cacgttggtt tgccgccaaa cgccgttttg ttgatgccga acgcgtgctg ctacgcctgc
      721 gtgacaccag cgcggaagcg aaacgcgaac tggatgaaat ccgtgaaagt ttgcaggtta
      781 aacagagtgg ctgggcgctg tttaaagaga acagcaactt ccgccgcgcg gtgttccttg
      841 gcgtactatt gcaggtaatg cagcaattca ccgggatgaa cgtcatcatg tattacgcgc
      901 cgaaaatctt cgaactggcg ggttatacca acaccaccga gcaaatgtgg gggacagtga
      961 ttgtcggcct gaccaacgta cttgccacct ttatcgcaat cggccttgtt gaccgctggg
     1021 gacgtaaacc aacgctaacg ctgggcttcc tggtgatggc tgctggtatg ggcgtactcg
     1081 gtacaatgat gcatatcggt atccactctc cgtcggcgca gtatttcgcc atcgccatgc
     1141 tgctgatgtt tattgtcggt tttgccatga gcgcgggtcc gctgatttgg gtactgtgct
     1201 ctgaaattca gccgctgaaa ggccgtgatt ttggcatcac ctgttccacc gccaccaact
     1261 ggattgccaa catgattgtc ggcgcaacgt tcctgaccat gctcaacacg ctgggtaacg
     1321 ccaacacctt ctgggtgtat gctgctctga atgtactgtt tatcctgctg acattgtggc
     1381 tggtaccgga aaccaaacat gtttcgctgg aacatattga acgtaatctg atgaaaggtc
     1441 gtaaactgcg cgaaatcggc gctcacgatt aatctcctca ggcttcctcc cgtcgcggag
     1501 gaagccacct cttgcagtca tcttttcttc gctctatcct ctgccgctat gaaaacatcc
     1561 cgtctcccta tcgccatcca acaggccgtt atgcgtcgcc tgcgggaaaa actcgcccag
     1621 gccaacctga agctagggcg taactacccg gagccaaaac tctcttacac ccagcgcgga
     1681 acctctgccg gaacagcctg gctggaaagc tatgaaattc gcctcaatcc cgttttgctg
     1741 ttggaaaaca gtgaagcctt tattgaagag gtagtgccac acgaactggc acatttgctg
     1801 gtatggaaac atttcgggcg cgtagcgcca catggcaaag agtggaagtg gatgatggaa
     1861 agcgtgctgg gcgttcccgc ccgtcgtacg catcagttcg aactgcaatc cgtgcgccgc
     1921 aacaccttcc cctaccgctg caagtgccag gagcatcagc ttaccgtacg ccgtcataat
     1981 cgcgtagttc gtggcgaagc cgtctatcgc tgtgttcact gcggcgaaca gctggttgcg
     2041 aaataaccac ctgaactatc aggaactttc ctgatctggc tgattgcata ccaaaacaac
     2101 tttcgctacg ttgctggctc gttttaacac ggagtaagtg atgtaccgtt atttgtctat
     2161 tgctgcggtg gtactgagca cagcattttc cggcccggca ttggccgaag gtatcaatag
     2221 tttttctcaa gcgaaagccg cggcggtaaa agtccacgct gacgcgcccg gtacgtttta
     2281 ttgcggatgt aaaattaact ggcagggcaa aaaaggcgtt gttgatctgc aatcgtgcgg
     2341 ctatcaggtg cgaaaaaatg aaaaccgtgc cagccgcgta gagtgggaac acgtcgttcc
     2401 tgcctggcaa ttcggtcacc agcgccagtg ctggcaggac ggtggacgta aaaattgcgc
     2461 caaagatccg gtctatcgca agatggaaag cgatatgcat aacctgcagc cgtcagtcgg
     2521 tgaggtgaat ggcgatcgcg gcaactttat gtacagccag tggaatggcg gtgaaggcca
     2581 gtacggtcaa tgcgccatga aggtcgattt caaagaaaaa gttgccgaac caccagcgcg
     2641 tgcacgcggt gccattgcgc gcacctactt ctatatgcgt gaccaataca acctgacact
     2701 ctctcgtcag caaacgcagc tgttcaacgc atgggacaag atgtatccag ttaccgacag
     2761 ggagtgcgag cgtgatgaac gcatcgcgaa ggtgcagggc aatcataacc cgtatgtgca
     2821 acgcgcttgc caggcgcgaa agagctaacc tacactagcg ggattctttt tgttaacccc
     2881 taccccacgc gtacaaccgc gtggggagac gacgcggatt tttaactatg cgtatccccc
     2941 gcatttatca tcctgaacca ctgaccagcc attctcacat cgcgctttgc gaagatgccg
     3001 ccaaccatat cgggcgcgta ctgcgtatgg ggccgggaca ggcgctgcaa ttgtttgacg
     3061 gcagcaacca agtctttgac gccgaaatta ccagcgccag caaaaaaagc gtggaagtga
     3121 aggtgctgga aggccagatc gacgatcgcg aatctccgct gcatattcac ctcggtcagg
     3181 tgatgtcgcg tggtgaaaaa atggaattta ctatccagaa atcgatcgaa ctcggtgtaa
     3241 gcctcattac gccacttttt tctgagcgct gcggcgttaa actggatagt gaacgtctga
     3301 acaagaagct ccagcagtgg cagaagattg caattgctgc ctgtgagcag tgtggccgta
     3361 accgggtgcc ggaaatccgt ccagcgatgg atctggaagc ctggtgtgca gagcaggatg
     3421 aaggactgaa actgaatctt cacccgcgcg ccagtaacag catcaatacg ttgccgttac
     3481 cggtcgaacg cgtccgcctg ctgattggcc cggaaggcgg tttatcggca gatgaaattg
     3541 ccatgactgc ccgctatcaa tttactgata tcctgttggg acctcgcgtt ttgcgtactg
     3601 agacaactgc gctcaccgcc attaccgcgc tacaagtgcg ttttggcgat ttgggctaac
     3661 ggagaagaat aatgatcaag ctcggcatcg tgatggaccc catcgcaaac atcaacatca
     3721 agaaagattc cagtttcgct atgttgctgg aagcacagcg tcgtggttac gaacttcact
     3781 atatggagat ggccgatctc tatttgatca atggtgaggc tcgcgctcgt acccgtactt
     3841 tgagcgtcga acagaactac gataaatggt acgagttcac gggtgagcag gatctgccgc
     3901 tggccgatct cgatgtgatc ctgatgcgta aagacccgcc gtttgatacc gagtttatct
     3961 acgcgaccta tattctggaa cgtgccgaag ataaagggac gctgatcgtt aacaagccgc
     4021 agagcctgcg cgattgtaac gaaaaactgt tcactgcctg gttctctgac ttaacaccag
     4081 aaacgctggt cacacgcaat aaagcgcagc taaaagcttt ctgggaaaaa cacagcgaca
     4141 tcattcttaa gccgctggac ggtatgggcg gcgcgtcgat tttccgcgtg aaagaaggcg
     4201 atccaaacct cggcgtgatt gccgaaaccc tgactgagca tggcactcgc tactgcatgg
     4261 cgcaaaatta cctgccagcc attaaagatg gcgacaaacg cgtgctggta gtggatggcg
     4321 agccggtgcc gtattgcctg gcgcgtattc cgcagggggg cgaaacccgt ggcaatctgg
     4381 ctgccggtgg tcgcggtgaa cctcgtccgc tgacggaaag tgactggaaa atcgcccgtc
     4441 agattgggcc gacgctgaaa gaaaaagggc tgatttttgt tggtctggat atcatcggcg
     4501 accgtctgac tgaaattaac gtcaccagcc caacctgtat tcgtgagatt gaagcagagt
     4561 ttccggtgtc gattaccgga atgttaatgg atgccatcga agcacgttta cagcagcagt
     4621 aacccacctt agcgagaagg atctcgttga gactctgagt gacagcgccc ttctttccac
     4681 gcatactggg cgctgttgct tttttgaacc aggaaacaga acctctgaca atgaatttac
     4741 agcatcactt tcttattgcc atgcctgctc tccaggatcc gattttccgt cgttccgtgg
     4801 tctacatttg cgaacataat accaatggtg caatggggat catcgtcaac aagccgctgg
     4861 aaaatctcaa aattgaaggg attctggaaa agctgaagat cacgccggag ccgcgtgatg
     4921 aatcaatccg tctggataaa ccggttatgc tcggcggtcc gctggctgaa gatcgcgggt
     4981 ttattttgca tactccgccc tccaattttg cttccagcat tcgcatttca gacaacacag
     5041 tgatgaccac ttcccgcgat gtgctggaaa cgctcggcac cgataaacaa ccgtctgacg
     5101 tattggtggc tctgggttat gcctcctggg agaaaggtca actggaacaa gaaattctcg
     5161 ataacgcgtg gctaacggcc ccggcagatc tgaatattct gttcaaaacg ccgattgcgg
     5221 accgctggcg cgaggcggca aaactgattg gtgtggatat tctcaccatg cctggtgtgg
     5281 caggacacgc ctgatgagtg gaaccttact cgccttcgac ttcggcacca aaagcattgg
     5341 cgtagcggtc ggccaacgca ttaccggcac cgctcgccct ttgcctgcaa ttaaagcgca
     5401 ggacggtacg ccggactgga acattatcga gcgtttactg aaagagtggc agccggacga
     5461 aatcatcgtc ggtttgccgc tgaatatgga cggcaccgaa cagccgttaa ccgccagagc
     5521 gcgtaaattt gccaaccgtg ttcatggtcg tttcggtgtt gaagtaaagc tccatgacga
     5581 gcgtcttagc accgtggagg cacgttccgg tctgtttgaa cagggcggct atcgggcgct
     5641 caacaaaggc aaagtcgact ctgcctctgc ggttattatt ctcgaaagct atttcgagca
     5701 gggatattaa ggcgatttac tagcgcacag atttctgaca atgtgctcgt tgtttcatgt
     5761 tggatgcggc gtaaacgcct tatccaacct acagtgagtg cgaattcaaa gttacgcttc
     5821 ccttgtaggc ccgataagct tgcgcatcgg gcatatttac aattcgtatc agacacagca
     5881 acgtcacaag cgcccttctt tcacccgctg ctgataactc tgctgaaacg ttatcatccc
     5941 cacctgctgc ccgatttgaa taacatgcgg taactggtgg gttttccctt cacgaatcaa
     6001 attccccacc gcgggtgtgt taatcagtaa ttcaaacagc gccacgcgtc cttcctgttt
     6061 atccacttcc agtttttgtg acaacactgc ccgtaaacta cctgccagtt gattacgcac
     6121 gggatctttt tcctgcgcag gaaatgaatc caccagtcgc tcaactgcct gcgctgcgcc
     6181 gcgcgtgtgt aatgtcgcca gcaccagatg cccggtttct gccgccgtca gcgccagacg
     6241 gattgtctcg ctatcacgca gctctccgag caaaatcaca tcaggatctt cccgcaatgc
     6301 agcccgcaat cccgatgcga aagtcataca gtgcaaacca atctcccgct gctggatcaa
     6361 acatcgctga ctggtataga gatattccac aggatcttcc agcgtcagaa tatgcgcatc
     6421 ggcatgttga ttgagatagc caaccatcgc cgccagcgtg gtagatttgc cgctccccgt
     6481 cgcccccgtc accagaatca ggccattctc gctcttgagt aattccggca ataccggtgg
     6541 tgcgccaagc tgttcgagct gcgggcagtg cgaaggtaac agccgtaacg ccagagaaat
     6601 accttgccgt tgtgcgaacg cactgccgcg caatcgctgg ttttccgcca gcgacacagc
     6661 aaaatccagc tgaccattct ccagcaatat tgcccgctga tcgtcatcca gccactcccg
     6721 cagtagctct tcgacgtccg gcgcgtcaaa cggcgcagct tccattctcc cgcgaatacg
     6781 ccatcgtgcg ggccaggcgc tgcacaggtg tagatccgag acgttatgct ttacactaag
     6841 ggccacaatt tcttccatat tcatactaag atcctcggaa aatgaacgat attgcgcata
     6901 acctggcaca ggtccgggac aaaatctcag cggctgcaac gcgttgcggc cgttctccag
     6961 aagaaattac gctgcttgca gtcagtaaaa caaaacctgc gagcgccatc gcagaagcca
     7021 ttgatgccgg gcagcgtcaa tttggtgaaa actacgttca ggaaggggta gataaaattc
     7081 gccactttca ggaactgggc ataacaggat tagaatggca ttttattggc ccgttgcagt
     7141 ctaataaaag ccgcctggtg gcagagcatt tcgactggtg tcataccatc gaccgtttgc
     7201 gcatcgctac ccgtctgaac gaccagcgcc cggcagaact tccccctctt aacgtactga
     7261 ttcaaattaa cattagtgat gaaaacagta agtccgggat tcaactggct gaactggacg
     7321 agctggcagc tgcggtcgct gaactaccgc gtttacgtct gcgcgggctg atggcaatcc
     7381 ctgcgcctga gtcagaatat gtaaggcagt ttgaagttgc acgccaaatg gctgtagcat
     7441 ttgccggact gaaaacgcgc tacccgcata tcgacacgct ctctctggga atgtcggacg
     7501 atatggaagc cgccattgcg gcaggtagca caatggttcg tatcggcact gcaatttttg
     7561 gtgcgcgtga ttactctaaa aaataaggaa ttaaaggaac gccatgaata cgttgacttt
     7621 cctgctttca acggtcattg agctgtatac catggtgctg ttattacgca tctggatgca
     7681 gtgggctcat tgtgattttt acaacccctt ctcacagttt gtagtgaagg taacgcagcc
     7741 gattatcggg ccactgcgcc gcgttattcc ggcaatggga cccattgata gcgcctcgct
     7801 gctggttgcc tatattctca gtttcgttaa agccatcgtg ctgtttaaag tggtgacctt
     7861 cctgccaatc atttggattg ccggtttatt gattctgctg aaaaccatcg gcctgctgat
     7921 attctgggtc ctgctggtga tggcgattat gagctgggta agccagggtc gtagcccgat
     7981 tgaatacgtg ctgattcagc tggccgatcc gctgctgcgc ccgattcgcc gcttgctacc
     8041 ggcaatgggt gggattgatt tctcgccgat gatcctcgtt ctgctgctgt atgtcatcaa
     8101 tatgggtgtc gcagaagtct tgcaggcgac cggaaatatg ctgctgccgg ggctgtggat
     8161 ggcgttatga gtgccgtaac agttaatgat gacggtctgg ttttacggct ctatattcag
     8221 ccgaaagcca gccgtgattc tattgtcggt ttacatggcg acgaggtaaa agtcgccatt
     8281 accgcgccgc cggttgacgg ccaggccaac agccatctgg tgaagtttct cggtaagcaa
     8341 ttccgggttg ccaaaagcca ggtggtgatt gaaaaaggcg aacttggccg ccacaaacaa
     8401 attaaaatca ttaatccgca acaaatcccg ccagaaatcg cggcgttaat taattaggta
     8461 tcctatgcaa aaagttgtcc tcgcaaccgg caatgccggt aaagtgcgtg agctggcgtc
     8521 gctgcttagc gacttcggtc ttgatatcgt ggcccaaaca gacctcggcg tagattccgc
     8581 tgaagaaacc ggcctgacct ttatcgaaaa cgcgattctg aaagcgcgcc atgcggcaaa
     8641 agtgaccggt ttaccggcaa ttgccgacga ctccggtctg gcggtagatg cgctgggtgg
     8701 cgcgccgggt atttactccg cgcgttattc cggtgaagac gcgaccgatc aaaagaatct
     8761 gcaaaaactg ctggaaacaa tgaaagacgt accggacgac caacgtcagg cgcgtttcca
     8821 ctgcgtgctg gtatatctgc gtcacgcgga agatcccact ccgctggtat gccacggtag
     8881 ctggccaggc gtgattactc gtgaaccagc gggcactggt ggctttggtt atgatccaat
     8941 cttcttcgta ccttccgaag ggaaaaccgc tgccgaactg acccgcgaag aaaagagcgc
     9001 catttcccac cgtggtcagg cgttgaaact gctgctggac gctttacgta atggttaaat
     9061 taccgccgct gagtctctac attcacatcc cgtggtgcgt gcagaaatgc ccgtactgcg
     9121 atttcaactc tcacgcgttg aaaggagaag tgccgcacga cgattatgtt cagcatctgc
     9181 ttaacgatct ggacaacgat gtggcttacg ctcagggccg tgaagtaaag acaatcttta
     9241 ttggcggtgg tacgccgagc ctgctttccg gcccggcgat gcaaacgctg ctggacggcg
     9301 tacgtgcgcg tttgccgctg gcagcggatg cagaaattac gatggaagcg aatcctggta
     9361 cggttgaagc cgatcgcttt gttgattatc agcgtgctgg tgtgaaccgc atctccattg
     9421 gcgtacagag ttttagcgaa gaaaagctga aacgacttgg gcgcattcat ggcccgcaag
     9481 aagcgaaacg agcggcaaat ctggcgagcg gtttagggtt acgtagcttt aacctcgatt
     9541 tgatgcatgg gctgccggat caatcactgg aagaggcgct aggcgatctg cgccaggcta
     9601 ttgaactgaa tccgccgcat ctttcctggt atcaactgac catcgaaccc aatacgctgt
     9661 ttggctcgcg ccctcctgta ctgccggacg atgacgcgct gtgggacatt ttcgaacagg
     9721 ggcatcagtt attaaccgca gcgggttatc agcaatatga aacttccgct tacgccaaac
     9781 ccggttatca gtgccagcac aatctcaact actggcgctt tggtgactac atcggtattg
     9841 gctgcggcgc gcatggcaaa gtgaccttcc cggatgggcg cattctgcgt accaccaaaa
     9901 cgcgtcatcc acgtggtttt atgcaggggc ggtatctgga aagccagcgt gatgtcgaag
     9961 ccgcagataa accatttgag ttctttatga atcgcttccg tttgctggaa cctgcgccgc
    10021 gcgtggagtt tagccagtat actggccttt cagaagaggt tattcgccca cagttagacg
    10081 aggctatcgc tcagggttat ctcacagaat gtgcggatta ctggcagata acggaacatg
    10141 ggaagttgtt tttaaattcg ctgctggagc tttttctggc tgagtaaatt tgtattgccg
    10201 gatgcggcgt gtacgcaaca tccggctaca actcttgcgt catcgcaaaa attacttaat
    10261 caccccattg cgccgcaatt gttcggtatc ttttttccag cgttcattaa cctggttgat
    10321 atcgtcaatt ttcatcaaaa tcgtgacttt tttactgttt atttctgaaa ctttcatcgt
    10381 taacggatat tgcccttttt cgagcgttat ccagtcatcc tgcaatttac ggctgagttt
    10441 ttgtgattgt agaaaagtct gtcgccgttg ttcgttgata tcaaaatctc gtttctgctg
    10501 aattggcact tcataacact cttcactctg tttatgacat gccgcaatcg ccggttgata
    10561 aacctctttg tcatatttaa ccgcgaacgc ctcgctatcg ttctgtttat taaaatcatc
    10621 acgctgttgg tgaatttttt taaaggcgtc ttcgcgggtc ctttgcttac cgtttgcatc
    10681 ttttccccaa tatgcatccg ctgctttctg cgccgcaacc atttgcttat caagcgcttt
    10741 tacatccttt ttcagtgcag gaatcgagtt attctggtta ttcaacgcag actcaagctg
    10801 actaaagcga taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat
    10861 atcgtcaacc acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga
    10921 ccagccagac gcgggcgttc ctttactggt taacatcgcc gagaatttca ccggtttatc
    10981 tttcgtccag gtctgttcga gcagctcgta atctgccaac tgaccgaccc agttatagag
    11041 atcgtcactg gaagagacat cgccttccac tgaccaggtg gcctgattac ccttagcatc
    11101 aagatttttt aaggtgatga catcaagctt gagaatgccg tgatactgct ttttgaattg
    11161 atcttttaag atattttcag ttggcggttc accatctgcc cggacattac cagtcatcaa
    11221 cataagcagc gccgtaccaa caatccattg ctttttcacc cgaaaaccct cgcgcggaat
    11281 aatttattcc ggtgattatc attagagtaa taaaaattgg caatggacgt gagtctgaag
    11341 tgaataagcc ccggcacgat accggggcga agtgattagt actgattgaa gatctgctgg
    11401 atctgctgcg gatctttagt ttgcgtcaga gccagttgca gcagaacgcg cgctttttgc
    11461 gggttcaacg tgccagaggc gacgaagccg tatttcgcat catccacttc ggcatcctga
    11521 gtggtagcgc ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcagcg
    11581 gttgcaaggg tgtcaaatac ggttttatac aggttgccgt tacccacgcc agcgctaacg
    11641 atgccatcat agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag
    11701 ttataaacaa tgccgacttt cggcagttca ttcagcttag agacatcgaa cggcgtgtcg
    11761 ctggtgtgct tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtaaccc
    11821 agaggaccgt agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca
    11881 tcacggccat caagtacggt gtcgttcatc actaccagta cgccacgatt agcggaggct
    11941 ttatcagctg cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatagac
    12001 gtggacgggc gcattgcacc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg
    12061 aggaagtaag cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcagtt
    12121 ttatcgcagt cggtgttaat ttttttcgcc agtgtcagcc agacatcatc gttcatgtcc
    12181 tgggagccaa tattcactac ctgctcgcct ttaacgttcg caatgtcctt cagttgcggc
    12241 accgcattaa ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg
    12301 gagtcaccac caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc
    12361 aatgctgcac cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc
    12421 atttcattcc tccagttacg tgaacgctac gcattatccc ttagctttgt atgggaaact
    12481 tgacgttaaa caatttacaa catgaatata ttttggagat ctacaaagtt agaggcaggt
    12541 aacaaaacga agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta
    12601 ttttaaattc ccgaccaggg ctttgcggct atcctccaga gtcacaacgc ggctacaaac
    12661 atctttgcca aactgctgga aatctttttc ctgctttttc cactcggttt ggattgagga
    12721 ttgcagaccg cccaggcttc ccagcacgtt ctgtaatggg ttaccgccgc ttttcagcac
    12781 cgctttcgcg cccatttcat taatgctgtc ctgtaaaatt ccgcccattg cctgattcac
    12841 taattgctgg ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc
    12901 gctgcgcgtt tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag
    12961 acggctgcgc attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat
    13021 acgggctttc tcgacgcggc ttttcgcgcc ttcatcaatc cacggcaggg tgctacgtag
    13081 ttcagcctga taatccttcg cctgctcgcg ctgggcagca ttcagggaat attgcttacc
    13141 gttatacatc acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg
    13201 cacggtttgc gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc
    13261 gtgagcggtc attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt
    13321 cttccctcaa gaacaaatca ggccagcatt tgctggcccc agattgataa caaagtgcgc
    13381 gttttccatg ccggatgcgg cgtaaacgcc ttatccagcc tactcgatcg tgcatcttta
    13441 taggcctgat aagcgtagca catcaggcag ttttgcattt gtcatcgccc gtatgctttc
    13501 ttagtcccac caaacgtcga aaagttcgct ggtgcgtacc tcttccagtt tgcgttcttc
    13561 cagccactta cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca
    13621 gatcagacct tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat
    13681 aacctcgtta ataaagtcat caacggtttt atcaatctgt tcttccgatg taccttccgg
    13741 gaatcgccat gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg
    13801 cagacgacgg ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact
    13861 ccgtgaccaa gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc
    13921 ggtacgtaat cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca
    13981 agcatatgtt ccgcataagg ttcccagtcg gtcgccatat ggaatatgcc ccccagctgc
    14041 agtttgcttt ttaccagctc ggcaaacggc acctgaacga tacggcgttt attatggcgc
    14101 gctttgtgcc acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc
    14161 attttatgca gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taagccctct
    14221 tcatgcgcag aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag
    14281 tcctgctcgg ggcgatcttt agccattgcc accaacgacg cgcccatgcc aaaaccaatc
    14341 tcaagcgtca ccggcgcttc acggccaaaa agcgcaggga aatccagcat atcttcgctg
    14401 aactcaacgc ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt
    14461 cgcccctggc ggcgcacaaa actacggata cggcgcagcg ggcggccgtt ttcatcaaat
    14521 tccggtgaga tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa
    14581 cgggcattat ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag
    14641 ttccggttta caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg
    14701 gtgaccatgc aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg
    14761 cggaaaacgc tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg
    14821 atgttgcaac aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggtgcgc
    14881 ttcccaacgg tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc
    14941 gggcttggct attacgcccg agcgcgcaat ctgcataaag cggcacaaca agtggcggcc
    15001 ttacacggcg gtaaattccc ggaaaccttt gaggaagttg cagcactgcc gggcgtcggg
    15061 cgttccaccg caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac
    15121 ggtaacgtca aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc cgggaaaaaa
    15181 gaggtcgaga ataaactatg gagtttgagc gagcaggtga cgcccgcggt tggcgtggaa
    15241 cggtttaatc aggcgatgat ggatttgggc gcgatgattt gtacgcgctc taagccgaaa
    15301 tgttcgctct gtccgctaca aaacggatgt attgccgccg ccaacaatag ctggtcgctt
    15361 tatccgggca aaaaaccgaa acagacgctg ccagagcgca ccggctactt tttgctgtta
    15421 cagcacgaag atgaagtatt gctggcgcag cgtccgccga gtggattgtg gggcggttta
    15481 tactgtttcc cgcagtttgc cgatgaagaa agtttgcggc agtggctggc gcaacgacag
    15541 attgctgccg ataacctgac gcagctgacc gcgtttcggc ataccttcag ccatttccac
    15601 ttagatattg tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaagac
    15661 aatgcgctct ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag
    15721 cgtttgttac agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga
    15781 tgatttatga gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat
    15841 tttcagctgt accccggcga actgggaaaa cgcatctata acgagatctc caaagaagcc
    15901 tgggcgcagt ggcagcacaa acaaaccatg ctgattaatg aaaagaaact caacatgatg
    15961 aatgccgagc accgcaagct gctggagcag gagatggtca acttcctgtt cgagggtaaa
    16021 gaggtgcata tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg
    16081 cctccggcaa cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct
    16141 cgcgctggct ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac
    16201 ctataacgaa gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc
    16261 ccacaatatt gagaacatct ggggcttcaa agaggtggtg atcgccggtc ctaaggacta
    16321 cgtgaaatac accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat
    16381 tactatcgaa accatcgccg ggacagaacc tgccgcgcat ttgcgccggg caataatcaa
    16441 aacgttgctg atgggggacg atccgagttc ggtcgatctc tattccgacg ttgatgatat
    16501 tacgatttcg aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat
    16561 tcgctgggaa ggtcgcgcga gcaacttcgc ggattatctg ctgaaaaacc gtctgaagag
    16621 ccgcagcaac gggctgcgta tcatctacag cgtcaccatt aacatggtgc cgaaccatct
    16681 tgataaacgt gcgcacaaat atctcggtat ggtccgccag gcgtcacgca aatatggcgt
    16741 tgatgagtcg ctgattctgg caattatgca gattgaatct tcctttaacc cgtatgcggt
    16801 cagccgttcc gatgcgctgg gattaatgca ggtggtacaa cataccgccg ggaaagatgt
    16861 gttccgctcg caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag
    16921 caatattgat accggcaccg cgtatctggc gatgctgaac aacgtttatc tcggcggaat
    16981 tgataaccca acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag
    17041 cgtgctgcga gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac
    17101 gccgggcgat gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta
    17161 tctttataaa gtgaataccg cgcaaaaatc ttaccgccgc cgataattcc actaaccgcc
    17221 cctgacgatg ctcaggggta aaaatgttat ccacatcaca atttcgtttt gcaaattggg
    17281 aatgtttgca attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcg
    17341 catcatctcg cacggtcaaa tgtgcttttt caaacactca tccgcatcac gatgtgagga
    17401 aattaacatg aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg
    17461 gggaagttgg ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc
    17521 ttctatcggc gcagtttata gctcactggg tatcgccgcg gtctttatgc ctgcgctgct
    17581 ggggattgtg gccgacaaat ggttaagtgc gaaatgggtc tatgccattt gccacaccat
    17641 tggcgctatc acgctgttca tggcggcaga ggtcactacg ccggaggcga tgttccttgt
    17701 gatattgatt aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctcata
    17761 ctatcgcctg caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg
    17821 gggcaccatc ggctttatca tggcaatgtg ggtggtgagc ctgtccggct tcgaattaag
    17881 ccacatgcag ctgtatattg gcgcagcgct ttccgccatt ctggttctgt ttaccctgac
    17941 tctgccgcat attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg
    18001 cctcgatgca ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat
    18061 gctgctgggc gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt
    18121 tgacaaagat ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc
    18181 gatttcgcag atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta
    18241 cggtattaag aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgctctgtt
    18301 tgcttacggc gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta
    18361 cggttgcgca ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag
    18421 cccggcaatt cgcgccagcg cgcaggggat gttcctgatg atgaccaacg gcttcggctg
    18481 tatcctcggc ggcatcgtga gcggtaaagt ggttgagatg tacacccaaa acggcattac
    18541 cgactggcag accgtatggc tgattttcgc aggttactcc gtggttctgg ccttcgcgtt
    18601 catggcgatg ttcaaatata aacacgttcg tgtcccgaca ggtacacaga cggttagcca
    18661 ctaattacgc aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca
    18721 acacataacc gtacaaccgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca
    18781 actccggcga aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt
    18841 gaaccgcccc accccagact tccccgggta ccacgcaaag cacgccaggt ggataaggca
    18901 acgccccttc tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac
    18961 cgcgaatata agcgctatgc gcatcctggg gattcatcac cactgacggg aaactctgct
    19021 ggcggaacat cgctttttgt aggtctttga cgtcgaaact gacatacaaa tcgtgcatct
    19081 cctgacacaa ctggcgcagg gtgtagtcgc gatagcgcac cggatatttg ttataaacgc
    19141 tcggcaacac ctcagccagc ggcgaatcat cctcaatatg ctgttcaaac tgcgccagca
    19201 tcgccaccag ttgtgccagc ttctcgtggc tttccgccgg agttaataag aacagaatgg
    19261 agttgagatc gcacttctcc ggcacaatgc cgttctcacg caggtagtgc gccagaatcg
    19321 tcgccggaac gccaaagtcg ctatattcgc cggtttcggc atcgataccc ggcgtggtga
    19381 gtaacagctt gcacggatca acaaaatact gatccgcggc atatccttca aagccgtgcc
    19441 aattcgcccc cggctcaaaa ctgaaaaaac ggcggtcgct ggctaacact gacgtcggat
    19501 aatcctgcca caatttgcca tcaacaacgg gcgggataaa cgggcggaac agcttacagc
    19561 gcgcaagaat agccttgcgc gactcaatac ccaactcaac gcactcagcc cacagccgac
    19621 gcccactctc cccttcatga attttggcgt taacgtccag ggcggcgaac agcggataga
    19681 acgggctggt agaagcatgg agcataaagg cattattcaa ccgcttatgc gggcaaaaac
    19741 gcgcctgtcc acggatatgg ttatcttttt tatggatctg cgacgtctgt gagaatcccg
    19801 cctgctgttt gtgcaccgac tgagtcacaa agatccccgg atcgttttcg ttaagttcta
    19861 acagcagcgg cgagctatcc gccatcatcg ggataaactg ttcgtaaccg acccacgcgg
    19921 aatcaaacag aatgtaatca cacagatgcc caacggtatc gatcacctga cgggcgttat
    19981 aaacagtgcc gtcataggtt cccagctgaa taatcgccag gcgaaacggg cgcggcaggt
    20041 cggctttttc tggcgcaacg tcgcgaattt gctggcgcag atactcttca ttaaaacagt
    20101 gcgcatcaat accgccaatg aaaccaaacg ggttacgtga agcttccaga tagaccggtg
    20161 tcgcccccgc ctgaatcaac gcgccatgat gattcgactt atggttgtta cggtcgaaga
    20221 gcaccagatc gccacgcgtt aacagcgcat tcgtcaccac tttattcgct gccgatgtgc
    20281 cgttcagcac aaaataggtt ttatcggcat gaaagacttt ggctgcaaat ttctgcgcat
    20341 ctttcgccga tccttcatga ataagcagat cgcccaattt tacgtcagcg ttacacatat
    20401 cggcgcgaaa gatgttctcg ccaaagaaat cgtaaaaatg gcgtccggca ggatgctttt
    20461 taaagaacgc accatgttga tgtccagggc aagcaaaggt gctattaccc atctcaacgt
    20521 actgcgtcag cgtgtcataa aacggtggca gcaaattctc ttcatactga caggctgcgg
    20581 attccagctc caaccactgc tgctcgttgc cgttgattac cgccgtaacg cccgcaggta
    20641 attcaacagc atgttcggaa tacaaaaaca ccggtagatg aaaaccggtg cgcttaagca
    20701 atgcaagaat gccactgcga ctatccgcag cggtaatgac gactgccgcg acgtccgtaa
    20761 aatcagtatc tcccaacgct accacgcagc gatgagtaga aagtcgggat accagttcac
    20821 tactggcggc aatattcatt gatttcataa gcgcaaaacc cgtttagggg aagtaagaat
    20881 accggacaag gtggaaaacc ctgccccatg agatatgggt caaactggtc accagctccg
    20941 accgccagac atcagtaaaa gcagaaacgc tctgatttta ctgttgtcct gcagtgagcg
    21001 tgcgttaact tcaccgcatg agcagtagca taaaaagaga agagtttcgc gcgaaacggc
    21061 gataagcgag agaatgtaag gaggtggcgt gcatcgggca aactccgtac agagaggaga
    21121 aaattcgcgc aatcatggca cctttcgcta aagcgtgcaa gccaggactt tgcgaacaaa
    21181 caagccatcg acggcgccaa aagttcataa taagaaaatc aaacaataca gctaacagga
    21241 actcttgtgg tcatcggccc ttttatcaac gcaagtgcag tcttactggg tggcgttttc
    21301 ggcgcactcc ttagccaacg cttaccggaa cgtatccgcg tctccatgac atcaattttt
    21361 ggtctggcat cgctggggat tggtatttta ctggtggtga aatgcgccaa ccttccggcg
    21421 atggttttag caaccttact cggagcatta atcggcgaaa tttgtctgct ggaaaaaggc
    21481 gtcaatacag tggtcgccaa agcgcataat ctgtttcacc actcgcgtaa gaagccagcg
    21541 catgaatctt ttattcagaa ttttgtcgcg attattgttc tgttttgcgc cagcggcacc
    21601 ggaatcttcg gggcgatgaa cgaagggatg accggcgatc cgagtatttt aatcgccaag
    21661 tcatttcttg atttctttac ggcgatgatc ttcgcctgct cgctgggtat tgcggtgtcg
    21721 gtgattagca ttccattact gatcatccag ttaacactgg cgtgggctgc cgcgctgata
    21781 ttaccgctga ccacaccgtc gatgatggca gacttcagcg ccgtaggcgg tttattgctg
    21841 ctggcaaccg gattacgcat ctgtggtatt aaaatgttcc cggtggtcaa catgcttcca
    21901 gcactccttc tggcaatgcc gctttccgcc gcctggaccg cctggtttgc ctgacaatgt
    21961 gtgcaatagc ggcaaagtga tgatagattg tgcagtctgc actaaattga agaaatttga
    22021 ttgacgagac gaggcgaatc aggtttaatg cgccccgttg cccggatagc tcagtcggta
    22081 gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc cgagtccggg caccacctta
    22141 catcaaagcc actggtcagg agtgagaacc ctgacgggtg tcatcgggaa ggtgtaagat
    22201 gtcgtgaatg cgctcatgcc atctcatttt ctcgatttaa ggagcatgtt ggtcgcatga
    22261 tccttctctc gatcattcgt tatgcttgca aagctttttc aggttgtcaa caacggcaaa
    22321 acaaagtcat gatctaaaat acatcagtaa gttctgccag ttctttttca agcactcttg
    22381 ccgtcccctt caaatcgatt tccacatact ccagagtggt ggaaacattc ctgtgcccga
    22441 gcatggtttt caccatataa agattacgtt ccggcgcact catcagcgta ctggccagcg
    22501 tatgccgaaa gcgatgaggt gatacaggga aaccgcactc tttcgttaga cgtctgaaaa
    22561 atccttttac cggttgcagt tccggaagtc cggtaacgaa cggagcttct gacatcaatg
    22621 ccacataacg atgataatgg aaaagataat cctcaggtcc tgccccccgt ttcatcgcct
    22681 catcaatcaa gtgcctcatt cccgggacaa gatgactgac catagggata cgccactcat
    22741 ggtgtgtctt acttccctcc gaacacagat caatccatct ttcatccaga ttaacatccc
    22801 gcagacgcag gtgaagtaac tgatttagcc tcatcccggt aaaacgcagc gtatttacca
    22861 caatcatcca gtaccatgcc ggacgaatcg cacaactacg ccagtgcaca ccatcagaac
    22921 gcttctcctc ttcatgaaac tgttcaagaa tcagcagaat tcgttttacc tgtttcttcg
    22981 tcagtgtttt cttacgtttc ttttcctgtc tgacattcat accatcaaac ggattctcac
    23041 ttaaattaag aagtgtagag gaacatgcaa aattgaaaat ggcacgcata tggcgaacct
    23101 tgttattcca tgtgtgcgtg gagcgattct gttttttcag aacctctcgt ctccattccc
    23161 tcacatgctg ctgccggata tcctccggta aaagggtctc gccaacaaac ttccggaatc
    23221 cattcagaac ctttcggtaa ctccactccg tagccactct cactgaacgg cagaaaaaat
    23281 attcatccat aagctcgttc cagcgcatgt cagcatgact taatttcata attaaaccct
    23341 ttacaaatag gttacatttt taccaaaata ctgacctcct gcgctcactg aaaaaaaagt
    23401 tcggactgtc attgagatcc cctgtatcca tcagaccacg tccttttatc agataacctt
    23461 tgagtatttt ataaccgtgt gttttccctg gtgcctcagc aattcgcgca tgataaaaac
    23521 gctgattatc ttttcgctta tggatattca gtcgttcaaa tgcagtctgt agcacctctc
    23581 tcttctcccc agggagttgc cgggctttca ggtaatcata aaaaatccct ggcaccggaa
    23641 tgaagacaaa gccggcaacc acatgaattt tatcgccttt attgttaaac gacagtgaac
    23701 cattcttcaa tccgtctttc agccattcaa gaaaccgctc tcctgttaaa tcaggattaa
    23761 cagcatcaga ttctgcttca cagtattcct gttgctcagc gtcttgatac gactgacaga
    23821 tttccccatg gcccgctgct gatatctctc ctgtcgatga aacaggcgca accacctcgc
    23881 cagatggcga gtcatcctcc cccttttccg gcgccctcgc ctcctccgca aacatgctta
    23941 ccagcatagc tgtatcttca tctgtcggta tactctccgt ttcacatcct gcatcgtcct
    24001 c
//
LOCUS       DAZGJT010000072        23969 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZGJT010000072 DAZGJT010000000
VERSION     DAZGJT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.072"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            180..818
                     /gene="leuE"
                     /locus_tag="ACHKFK_003129"
     CDS             180..818
                     /gene="leuE"
                     /locus_tag="ACHKFK_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO9053409.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NSETKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            991..1350
                     /gene="yeaR"
                     /locus_tag="ACHKFK_003130"
     CDS             991..1350
                     /gene="yeaR"
                     /locus_tag="ACHKFK_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO9053410.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            1354..1536
                     /gene="yoaG"
                     /locus_tag="ACHKFK_003131"
     CDS             1354..1536
                     /gene="yoaG"
                     /locus_tag="ACHKFK_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO9053411.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            1684..1932
                     /gene="yeaQ"
                     /locus_tag="ACHKFK_003132"
     CDS             1684..1932
                     /gene="yeaQ"
                     /locus_tag="ACHKFK_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO9053412.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(1992..2066)
                     /gene="yoaJ"
                     /locus_tag="ACHKFK_003133"
     CDS             complement(1992..2066)
                     /gene="yoaJ"
                     /locus_tag="ACHKFK_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO9053413.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(2069..2167)
                     /gene="yoaK"
                     /locus_tag="ACHKFK_003134"
     CDS             complement(2069..2167)
                     /gene="yoaK"
                     /locus_tag="ACHKFK_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO9053414.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            complement(2200..3225)
                     /gene="dgcP"
                     /locus_tag="ACHKFK_003135"
     CDS             complement(2200..3225)
                     /gene="dgcP"
                     /locus_tag="ACHKFK_003135"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO9053415.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCVAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     THSALHS"
     gene            3408..3662
                     /gene="yoaF"
                     /locus_tag="ACHKFK_003136"
     CDS             3408..3662
                     /gene="yoaF"
                     /locus_tag="ACHKFK_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO9053416.1"
                     /translation="MKIISFVLPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            complement(3684..4031)
                     /gene="yeaO"
                     /locus_tag="ACHKFK_003137"
     CDS             complement(3684..4031)
                     /gene="yeaO"
                     /locus_tag="ACHKFK_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO9053417.1"
                     /translation="MNIQCKRVYDPAEQSDGYRILVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYSAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4086..5267)
                     /gene="nimT"
                     /locus_tag="ACHKFK_003138"
     CDS             complement(4086..5267)
                     /gene="nimT"
                     /locus_tag="ACHKFK_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO9053418.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRYAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISQGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            5364..6185
                     /gene="nimR"
                     /locus_tag="ACHKFK_003139"
     CDS             5364..6185
                     /gene="nimR"
                     /locus_tag="ACHKFK_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO9053419.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVKHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLRQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            complement(6142..6588)
                     /gene="yeaL"
                     /locus_tag="ACHKFK_003140"
     CDS             complement(6142..6588)
                     /gene="yeaL"
                     /locus_tag="ACHKFK_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO9053420.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            6758..6862
                     /gene="yoaI"
                     /locus_tag="ACHKFK_003141"
     CDS             6758..6862
                     /gene="yoaI"
                     /locus_tag="ACHKFK_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO9053421.1"
                     /translation="MNDQMFVETLIITSSFFAIAAVLVLSVLLIERTG"
     gene            complement(6863..7366)
                     /gene="yeaK"
                     /locus_tag="ACHKFK_003142"
     CDS             complement(6863..7366)
                     /gene="yeaK"
                     /locus_tag="ACHKFK_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO9053422.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(7409..8899)
                     /gene="dgcJ"
                     /locus_tag="ACHKFK_003143"
                     /pseudo
     CDS             complement(7409..8899)
                     /gene="dgcJ"
                     /locus_tag="ACHKFK_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
     gene            complement(9080..10555)
                     /gene="cdgI"
                     /locus_tag="ACHKFK_003144"
     CDS             complement(9080..10555)
                     /gene="cdgI"
                     /locus_tag="ACHKFK_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO9053423.1"
                     /translation="MIRSTRISLGLFFNYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQGFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTHNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            complement(10702..11985)
                     /gene="yeaH"
                     /locus_tag="ACHKFK_003145"
     CDS             complement(10702..11985)
                     /gene="yeaH"
                     /locus_tag="ACHKFK_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO9053424.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKILPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            complement(12098..14032)
                     /gene="yeaG"
                     /locus_tag="ACHKFK_003146"
     CDS             complement(12098..14032)
                     /gene="yeaG"
                     /locus_tag="ACHKFK_003146"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO9053425.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAEHYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            14468..15214
                     /gene="mipA"
                     /locus_tag="ACHKFK_003147"
     CDS             14468..15214
                     /gene="mipA"
                     /locus_tag="ACHKFK_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO9053426.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDTSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            15304..16158
                     /gene="yeaE"
                     /locus_tag="ACHKFK_003148"
     CDS             15304..16158
                     /gene="yeaE"
                     /locus_tag="ACHKFK_003148"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO9053427.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALIGLRENVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFALEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPRCQKQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     HNISAAQVLLAWVISHQGVMAIPKAATVAHVQQNAAVLEVELSSAELVMLDKAYPAPK
                     GKTALDMV"
     gene            complement(16209..17093)
                     /gene="yeaD"
                     /locus_tag="ACHKFK_003149"
     CDS             complement(16209..17093)
                     /gene="yeaD"
                     /locus_tag="ACHKFK_003149"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO9053428.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGIPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFHVGKTCEIDLEAHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(17177..18172)
                     /gene="gapA"
                     /locus_tag="ACHKFK_003150"
     CDS             complement(17177..18172)
                     /gene="gapA"
                     /locus_tag="ACHKFK_003150"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO9053429.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            18514..18927
                     /gene="msrB"
                     /locus_tag="ACHKFK_003151"
     CDS             18514..18927
                     /gene="msrB"
                     /locus_tag="ACHKFK_003151"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO9053430.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            18969..19241
                     /gene="yeaC"
                     /locus_tag="ACHKFK_003152"
     CDS             18969..19241
                     /gene="yeaC"
                     /locus_tag="ACHKFK_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO9053431.1"
                     /translation="MNFDDIINSMTPEVYQRLSTAVELGKWPDGVTLTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            19611..20687
                     /gene="ydjL"
                     /locus_tag="ACHKFK_003153"
     CDS             19611..20687
                     /gene="ydjL"
                     /locus_tag="ACHKFK_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO9053432.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCFVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFYVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            20714..22093
                     /gene="ydjK"
                     /locus_tag="ACHKFK_003154"
     CDS             20714..22093
                     /gene="ydjK"
                     /locus_tag="ACHKFK_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053433.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMGVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLIAM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSIIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            22110..23153
                     /gene="ydjJ"
                     /locus_tag="ACHKFK_003155"
     CDS             22110..23153
                     /gene="ydjJ"
                     /locus_tag="ACHKFK_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFO9053434.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLTMAEQLGATVVI
                     NGAKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            23174..23862
                     /locus_tag="ACHKFK_003156"
                     /pseudo
     CDS             23174..23862
                     /locus_tag="ACHKFK_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
     gene            complement(23880..>23969)
                     /locus_tag="ACHKFK_003157"
     CDS             complement(23880..>23969)
                     /locus_tag="ACHKFK_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9053435.1"
                     /translation="LGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         6309 a         5514 c         5868 g         6278 t
ORIGIN      
        1 tacgccagta ttttgccgag cactacccga atttttcact ggagcatgcc tgattaatga
       61 ttcaattatc gggttgatat cgagttaaaa cctgattttc tcctttctaa gccgctacag
      121 attggttagc atattcacct ttaatcgcgc atgatcgaaa gataattaaa gaggttaatg
      181 tgttcgctga atacggggtt ctgaattact ggacctatct ggttggggcc atttttattg
      241 tgttggtgcc agggccaaat accctgtttg tactcaaaaa tagcgtcagt agcggtatga
      301 aaggcggtta tcttgcggcc tgtggtgtat ttattggcga tgcggtattg atgtttctgg
      361 catgggctgg agtggcgaca ttaattaaga ccaccccgat attattcaac attgtacgtt
      421 atcttggtgc gttttatttg ctctatctgg ggagtaaaat tctctacgcg accctgaaag
      481 gtaaaaatag cgagaccaaa tccgatgagc cccaatacgg tgccattttt aaacgcgcgt
      541 taattttgag cctgactaat ccgaaagcca ttttgttcta tgtgtcgttt ttcgtacagt
      601 ttatcgatgt taatgcccca catacgggaa tttcattctt tattctggcg acgacgctgg
      661 aactggtgag tttctgctat ttgagctttc tgattatttc tggggctttt gtcacgcagt
      721 acatacgtac caaaaagaaa ctggcgaaag tgggcaactc actgattggt ttgatgttcg
      781 tgggtttcgc cgcccgactg gcgacgctgc aatcctgatg ctttcagccc gcgttgccgc
      841 gggcttccca cctgtaattc tccctgattc ttctctgata cggtgctaaa aagtaaccaa
      901 taaatggtat ttaaaatgca aattatcagg cgtaccctga aacggctgga ataaaccgtt
      961 ttcagcgcat tcaccgaagg agggaaaagg atgctgcaaa tcccacagaa ttatattcat
     1021 acgcgctcaa cgcctttctg gaataaacaa actgcgcctg ccggaatatt cgaacgtcat
     1081 cttgataaag gaacgcgccc aggggtttac ccacgccttt ccgttatgca tggggcggtc
     1141 aaatatctcg gctacgctga tgaacacagt gcagagcctg atcaggtgat ccttatcgaa
     1201 gcggggcagt ttgcggtgtt ccctccagaa aagtggcaca acattgaagc catgactgac
     1261 gatacttatt tcaacattga cttcttcgtt gctcctgaag tcctgatgga aggtgcgcaa
     1321 caacggaaag tcattcataa cgggaaatga atcatgggca aagcaacgta taccgtgacc
     1381 gtcaccaata acagcaatgg cgtttctgtc gattatgaaa cagagacgcc gatgactttg
     1441 ctggtgccag aagtggcggc tgaagtgata aaagatctgg tgaataccgt gcgttcttat
     1501 gacacggaaa acgaacatga tgtttgtggt tggtaattat cactgatcgt tgatggcgca
     1561 atttctgcgc catcgcaaaa aattcctccg tgatgctcgt cacaaatctt accctcccga
     1621 tgttgtgcta tgctttttat cagcgactaa cccgctaatg caaaacagat aagtgaggaa
     1681 acaatgggca ttctgtcatg gattattttt gggcttattg ccggtattct ggcgaagtgg
     1741 atcatgccag gtaaagatgg tggtggattc tttatgacta tcctgctggg gatagtcggt
     1801 gccgtagtcg gcggatggat cagcacgctg tttggctttg gtaaagtcga tggcttcaat
     1861 tttggcagct tcgtggttgc cgttattggt gcgattgttg tgctatttat ctacaggaag
     1921 attaaaagtt aacgcgtaaa ttgcacaaag gctgcacaca ggcagccttt gctgtttttt
     1981 tagatgtgac gttaccacca tcccagctca gtgccgagta cgacaataat cgccacaccc
     2041 atcataataa tcgttgtttt tttcatcttt atgctcccgg ggcagcatag ccgccaataa
     2101 aaaaccatgc taaaaatacg accgctgtaa taaagattac aaccggaaaa ataataccga
     2161 ttcgcatgtt atcaccaatc aaaaggatgt gaatacgcct caggaatgta gcgctgaatg
     2221 cgtgacaaaa ggtgttttct gtttatgttt tttctcctga tacatcgcga tatcggcagc
     2281 atgcagggca ctgtctgcat cggtcgtttc aggatcgact tctactatgc caagactggc
     2341 accaggataa tacaaatcaa catcacctaa gtgatattct ccgcgtattt gttgttgaat
     2401 acgttctcgc agcgacgaaa tatccgcatt ctcgttgttc agtgaaacaa ccaaaaactc
     2461 atcaccgccg agacgaccaa taacttcgcc attttgctgg agcgtattaa ggcgctcgcc
     2521 aacctgaatg agaaacagat cgccactatt atgaccaaaa cgatcattga ttaatttgaa
     2581 gttatccaga tcgataaacg cgatcattat cttatggtta agatgccggg cgagagaaaa
     2641 cagcgtcgtc agattttcaa aaatcgcccg ccgattcggt agcccggtta acgagtcggt
     2701 atacgattgc gcaatcagcg cagcattagc ttcgcgcagc tgttcaacca gtgcctcttt
     2761 ttgaatatat tgtgcaatca gtccggcgaa caactgtaag acctgttccg cgcgttcact
     2821 ccactggcgc ttctcactgc tggcggcgca aagcgtgcca tagaatgatc catccggtaa
     2881 gtgaattggc gtgctcagaa atgtggtgat gccaagattg cgtgccgcaa cacagtcgcc
     2941 ccaacggtcg gggacttcat cgctgaaaaa acagttttca tcaatggcgc gtttgcataa
     3001 cgagtaatcc cacgagacgg taaaattctc cgggatgtgc attttctggc tattacgggc
     3061 aaacattata tgctgcaggc gcgcttcgac atccactttg gtcaggtagg ttgactccat
     3121 atcagtgacc atttccagca tctccagaag ctgtcggacc aggctttcca gtgactgttc
     3181 tttggcaagg gattgcgaga cgcgggcgat aatctgatct gacacgaaac aactccagga
     3241 tacggcgggt aaatgctgat tatttaatct ttgcaggcat gataaaacat catcaacaaa
     3301 gatgaaacaa ggcgtaatat gatttgagtg aatgtaataa aaatagtgct gcaaaacaaa
     3361 acgataccgc gatactctaa gcctggataa atatgtgaga tcaataaatg aaaattatca
     3421 gttttgttct gccttgcttg ctggttctgg ctggttgttc aacctcttct cagccagaag
     3481 cacctaaacc gccgcagatt ggtatggcaa atccggcgtc ggtctattgc cagcagaagg
     3541 gcgggacgct cattcctgtg cagacagcgc aaggggtcag caacaattgc aaattaccgg
     3601 gcggtgaaac gattgatgaa tgggcattgt ggcgacggga ccatccggct ggtgaaaaat
     3661 aaccgccgga tgctgtacta aaatcacaag ctacgtagcc agtctgccag caccagcgca
     3721 tggttctgcg tggtgttttt tgctgagtag agcagggtca gcggctgttt tttggcgatg
     3781 tctgccagcc gctttccttc ttgctcgtgt tgcgccagtt ctgcaagata ttgctcgcga
     3841 aaggttgcaa aatcgacgac ttcgccgtga aaggctttgc gcagctccgt tgacggcgtg
     3901 atttctttat cccactcatc aagggctaaa tcggtttttt tgataccgcg cggccagagg
     3961 cggtcgacca gtatgcgata accatcgctc tgttcagccg gatcataaac gcgtttgcac
     4021 tgaatattca tcgtgtttgt tctccctttc acgcatgatt tgtcacgtca ctatagcacc
     4081 ggatattagc gaatttcttt gtctctcccg gcgcaaagtc cgaaaatcgc catcagtagt
     4141 gaaagtatgg caacacccat cagtggtaca gaccagttac cgttagcatc gtgtatttta
     4201 cccatcagcg gcggcccaca ggctgccagc aaatacccga cggattgtgc catccccgag
     4261 agtgccgccg cctgatgcgc agaacttgcc cgcagaccaa tgaacgtcaa ccccagtatc
     4321 attgttgcgc cggaaccaaa accgaaaagc agagtccagg tgatcgcgtg tgctggcata
     4381 aagcagagcc caaccgcgcc aactgcgcac atcaaggcaa cgaacgctgc aataccacgc
     4441 tgatctttca catgatgtaa gaaaagtggg atcagcaaac cgggagctgc tgtggctagt
     4501 tgcagcaaac catgcagtga acccgcctgt gcttcgctat agccttgact gatgaggatc
     4561 gccggaagcc agccaataat cacgtaatag acgagtgagt tgatcccaag aaacaatgtt
     4621 acctgccagg caagcggcga acgccagata ccccgagtat gtaaggcgcg tgaggtactc
     4681 aaatttgcat gttgttgact tcgccactgt ggcagccata aaaaaagagc cagcagagga
     4741 aaacacatca gcatgagcaa cgcgccttgc cagccaaaac cgttcaaagc cagcggcact
     4801 accatagccg atcccagtgc cgccgcagct cccattgtca gggaatatgc gccggtaagt
     4861 ctggcgacgg aatgagggaa atcgcgttta attaatcctg gcagtaagac attgcctaat
     4921 gcaatcccac cgccaatgac cgctgtaccg ccaaataata agtaaggcga agggagagag
     4981 cgaattgcga tacctgcaca gatcaaaagt aacgcggcaa acaggctacg ttccatacca
     5041 aaacgtcgcg ccaccggggc ggccagcggt gagattagcg caaaggctaa taatggcagg
     5101 gtggtcaata agccggtttg cgccgtcgtt agcgagtaag cgtaacgaat cgtatccagt
     5161 aacggtgctg cgccggtaaa ggtgacgcgt aatgttgtgg caatcatcag aatgccagcg
     5221 ataaggacaa tcctgttttt gccgcttaat gaagttgaac aggtcatatg cttctcattg
     5281 ttacaaacga ggctgtacct taccgttttt attgttatgt tgaatcaagc taaaatgaca
     5341 aaatatcgct aaattcggac aaaatgatgc acaggttgaa tctcaatggc tatgaacccg
     5401 accgacacca tgaagccgcg gtcgcatttt gtattcacgc cggaacggat gagttaacca
     5461 gtcctgtaca tcagcaccgc aaagggcagt tgatactggc actacatggt gcaattactt
     5521 gtacggtgga aaatgctttg tggatggtgc cgccacaata tgcggtttgg ataccgggcg
     5581 gggtgaaaca tagtaatcaa gtgacagcta acgctgaact ctgttttctg tttatcgagc
     5641 ccagcgcagt gacaatgccg acaacatgtt gcacattgaa aatatcgccc ttatgccggg
     5701 aattaatttt aaccttggca aacaggacga cgactcagcg tgctgagccg atgacccgca
     5761 gattaattca ggtcctgttt gatgaactcc ctcagcaacc acaacaacag ctgcacttgc
     5821 ccgtttcttc tcatcctaaa atccgcacga tggtggagat gatggcgaaa gagcccgtcg
     5881 agtggggctc attgaggcaa tgggctggct tttttgcgat gagtgaacgc aatcttgccc
     5941 ggctgatagt gaaagagacg gggttgagtt ttcgccagtg gcgtcagcag ttacagctga
     6001 ttatggcgct tcagggatta gtgaaaggcg atacagtaca gaaagttgct catacactgg
     6061 ggtatgattc aacaacggcc tttattacta tgtttaagaa aggcctgggg caaacgccag
     6121 gccgatatat tgcgggatta actactgttt ccccacaatc agcgaaacca gacccgcggc
     6181 aataagcggg ccaaccggta cgccgcgaaa cagcgccacg cctaaaacag tgcccaccag
     6241 cagcccggcg accagttgcg gctggctgcc cattaacgtc acgccgcgcc cccccagcca
     6301 ggagacaata acgccgactg caattgccac cagcgatttc cagttcagaa acgagtgaat
     6361 cagggttgag ggcggtagcg tcccgctggc gatgggggcc ataacgccaa tcgtcagaat
     6421 gattatcccg atacttagcc cctgtttttc aatccacggg aaaaaggtgc ttaacggtgt
     6481 gacgcggaca ataatcaaca ccagaattga aacggcgaca gtggtgttat gactgataaa
     6541 gcccagcgca gctaacccga gcaggatcag caaagtgaca tcaaacatcg tggtttcctt
     6601 gccaaaaaat aataagcctg cttactttac gcttgctgca ggcaggaagt agaaaatctt
     6661 tacgtttttt gcataaaaaa tcagacgaac tgtcatcaaa ctgccatttg tgtacgttaa
     6721 caataacgtg aaaacagcta tctcccgggg gcatactatg aacgatcaaa tgttcgtcga
     6781 gacactgatt atcacgtcat cgttttttgc cattgctgct gtgctggttt tgtccgttct
     6841 gcttattgaa cggacaggct agttacgcag tgcggcggaa attgaccagt tctggttgcg
     6901 caatacgcaa ataatccgcg gttttcaaaa taacggattt atccagcatg cctgcattga
     6961 aggcgatttc atcaaatcgt tcgaagagta gggggtcggc aaccagtttg agttttggat
     7021 ggaagctgaa aggggggatc gcgccgaaga cacagcctgt gagttcatcg acttccgccg
     7081 ggctggccaa cgaagcgcgt aatccgccta tatggctggc gagttggctc agatcggcct
     7141 gttgatcggc agcgaggatc gccagaacat gttgatttac gccattgcct ttgactttgc
     7201 ataccagtgc cttagccccc tggcctaaag cggtgccacg aatttctgac accgcttcac
     7261 atttacctac cgcttcatgg gtaacgacgc ggaagttagc accttcttgt gataataaag
     7321 cgattaaccg ctgatgcgtc acgcttcctt tagccatttc agtcatttca tgctcctgag
     7381 attacaagca aacaaccaca gaagattatc atgaacggct gtttttgttc tgcttattga
     7441 catataaacg ctcatcggaa gctttatacg catcatgtaa cgtatcgttt tctttcatcg
     7501 cgtaaatacc ggaagagaag ccgatctctt tctgcggcgc gatatgttgc agacgttttt
     7561 cgatacgttc aggcagttgt gctgcaattt gcggcgtcga atcgacaaga atgatgcaga
     7621 attcatcgcc accgagtcgg atggcataat cacttttacg aatcgactgt ttaatcgcct
     7681 gagctaataa cgtaatcgct aaatccccct cctgatgacc gagggtgtca tttatttgct
     7741 ttaacttgtc catgtcaata gcaataaaca tcaccgaaga accggattgc accagtttct
     7801 gcaaccgctg ctccagttca ggggttaaaa ttttgcgatt atagagtcca gtcatcgcat
     7861 cactaatatt ttctcgcgac acattttggt ataagcggaa gtgcatccgc accatattca
     7921 gcaaaattaa cgccgtcaaa atccagaaca gaacgctttt ccacgaagac gtgataaagt
     7981 aaagaatatc aatggataac gagacacgaa tgccgcccgg taagtcatgg acgtaactga
     8041 catactggaa cagattatct tcgctctggt tgatgataat gtcgcgcccc gaatctgtat
     8101 cggttagcgt gacattgaga aaacgccaga ggagagggcg gtcatgagtt taaaagatat
     8161 tccgcagatt gtttttgtta atatccagca gcacaatccc ttttagctgc cctgcgacat
     8221 ataccggcgt cagcaaactc atcacgttct gtttagtacg ctgatcctgg taaatgctcg
     8281 ataatacggt acgcccggta aatacttttt caatatcttt gcgatcgata ccaatacttc
     8341 ctgactgcag gaaggaccag ttattcatcg caaagtattc aacattaacc ggctcataaa
     8401 aatagacata gcggtcattg agatcgagat aatagtgaaa tttctcttcg gccatcccaa
     8461 gacctgaacc gtaatcatcc tggtggcggt tgccttccac tgcctgatca aaagcgggta
     8521 atagtgctgc agcttccaca attgtgtcgc agtcagtgga tgcggtttgt agtgtgccgc
     8581 gtaagccctg gtatttatga gcagtcaggt ttaagccatg ggtattattg gcagactcaa
     8641 aagcctcgca gatagcgcgc cgttgttctg gcggtacttc cgactgctca gcagcgagag
     8701 cgcgcatcac atgcgcggca atactctgat tctgatactt atcataaaga aaggaagagt
     8761 ctgctctctg cacgatatag cgcagatagg cactcattgc tctgtcgctg gcgacaagtt
     8821 caaaaataag gaaggaagat gtcagcacaa tgacacttgc ggcgataaaa tgccggagca
     8881 ttctatggtg caatttcatg ataaatggca ctccttcggt tagtgaaacg agaaaaccag
     8941 ataaagttcg attgcggcct ggcacagggg cgctaattat actcatctct gcgccgctgg
     9001 ggaggggaaa tttaggggta atagtaataa attgtatgca aaagccacgc aaaaagcatg
     9061 gctgtgaata tttttgcact taaatatgat gaataatcac tttgttgccc ccctcgcttt
     9121 tcgcttcgcg taaggcttta tctgcttccg tcatgacaag cgagatatta tgcgtgtcac
     9181 ccgttgaaaa aaccgtgcca atactgatgg tcatttgttc aggaacatca tatctgttac
     9241 taaagccggt caggagttcg acatttttac gcatacgttc cgcaatgatt tttgcctggg
     9301 cactattgag ttcggtaaat agcaagccaa acacctcacc ctccagtcgc gctaaaatat
     9361 catcggggcg gatgctttgc tggataatat cgacaattga taccagcact ttatcaccca
     9421 cacgatgtcc ccattgggca ttgatgcgtt tgaactgatt aatatcaacc agcatgacac
     9481 aaaagtcctt aacaacaggt cgcgacaata atgactctac gctgttgaaa aagtagcgcc
     9541 gattataaat attagtcagc acatcatgaa ctgccagttt gctggagagt tgcagttcct
     9601 gaaaaatgtt ataaatcaga aaagacacaa caaacagttt actggaaact tcaatcccgc
     9661 gactgatata ccagatggtg taattatact catccagagt cagtagaagt aagttgcagc
     9721 atagcgatgc cagacataat aatgctatta acggccatag ttcgctggct aatcgtgtac
     9781 gcatgataat aaagaacagt aaaaatgccc acaaacaaac caatattttt gtatagttga
     9841 ttccccaggt cgcagtatgg ttgtccggac agtaatcgac gacatacaaa gaatagtcag
     9901 cgttataact actcagattg tgtgccagaa ggggaaaaac taaaaaagga atcagcgcca
     9961 gcaacagaat gccagttttt ttcttattat tgtcaaggat gttgtctttc ccataacaaa
    10021 agagcgccag cgaggttaaa caaatgaaac tgagttggcg gaaaatatag aaaatagaaa
    10081 catcattcga ctttgtctgg atcagtgtac tgccgttgat tggtttatgg ataattacaa
    10141 tggtttcaac taaatagata aggccactta aaaatgcaag gcttaaaatg agtgttggta
    10201 aaatttgttt gttagtgaaa tacttcatta acatgaaaag aaaaatgacg gcgttaagga
    10261 aaaacaaagc cacaacaacc atcaggtaat tcatctgtgg gataagtttt acatttgtgt
    10321 tttcgttata taaggtagta atgcaaaata aaattaacgt cagcaataag ctgaaaataa
    10381 aaaagccttg agcttttaat ttttgtgtac ccatagaaac cataaaaagg agagcaatgt
    10441 gagtgctgga ttttattgat ggcagagata tatagttttg accaggatca attttcgtta
    10501 acgataaaaa ataattaaag aataacccca gggaaatacg tgttgaccga atcatttttc
    10561 cgaatgattg ttttctggaa ctaaattgtg taaaggttat gctgtcatat ttaaagcgat
    10621 tgtaagctaa tgtatgtaat aaatgagata atttacagtg agctaaaaat cagccaggcg
    10681 ataatgaccc ggctgacagt tttagtcttt agctgttgca ttttgtttat gaaacagttc
    10741 acggaacacc ggataaatat catcctggtc gcggatatgc tgcatcgcaa agttgtcgaa
    10801 agtagattgc agatgctcat attctcgcca cagtgtctga tgcgcgcgac gtgtaatttc
    10861 gatatagctg taataacgaa caacgggtaa tatttttttc gccagaattt catggcaaag
    10921 cggagagtca tcggcccagt tatcgccgtc tgatgcttgg gctgcgtaaa tattccactg
    10981 tgccgggtta taacgctctt tcactacctc atccatcagt ttcagggcgc tggaaacaat
    11041 ggtgccacca gtttcctgcg agtagaaaaa ctcatgttca tcgacttctt tcgcctgggt
    11101 atgatggcgg atgtatacga cttccacgtt cttatacgtt ctgctgagga acagatacag
    11161 cagaatataa aaacgtttag ccatatcttt agtggactga tccattgaac cggaaacgtc
    11221 catcaggcaa aacatcactg cctggctgga gggatcgggc cgtttttcgt agttcttgta
    11281 acgtaaatcg aaggtgtcaa tgaaagggac gcgttcaatt ttggcacgta attctgcaat
    11341 ttctttgcgc agacgttcct cttccagcag ttgcgcaggt tcactgttgc tgatgatggc
    11401 caaattctct tccagcgcat gaagttcccg ccgcttgccc gccgtcatag ctgtgcgccg
    11461 cgccagtgag ttctgcaatg aacgtacaac gctgatattg gccggaacgc cgttagcggt
    11521 ataacccgcc cgatgagttt tatattcggt cagctggcgt tgttggtttt gtttcagatt
    11581 cggtaaggcc aaatcttcaa agagcagatc aagatactca tctttcgaaa tctgaaagac
    11641 aaattcatcc tgaccttcac catcctggct ggcctggccc tgaccactgc cggaacctcc
    11701 gccaccaccc tggggacgtt caattcggtc gttctggacg aaatggtcat tgcccggatg
    11761 cacacggtgg cgcaggccac cacgcccctg atgaaacatc ggttcgctaa tatcttccgt
    11821 gggaatggat atggactcgc cgctgtcgac gtcagtcacc gaacgcttat taatggcctc
    11881 ggagatcgac tgtttaattt gcgctttata acggcgtaag aaacgctggc gattcaccat
    11941 gcttttattt ttgccgttca gacgccggtc aataaaccag gtcatatgcc ccccgtacta
    12001 catttgccaa cggtatgcta cgcttgtgtt gccgcttctc tggcccacgg ttcgctgtcg
    12061 ttgtaggccc ggtagcatgt gtaccgggcc agggttgtta agacgattta cgtacgcgca
    12121 aataccattc gcacagtaaa cgcacctgtt tacgggtgta gcctttttcc atcatacggt
    12181 cgacaaagtc gtcgtgtttc ttctgctcgt cggttgacgt tttggcgtta aacgaaataa
    12241 ccggcaacag ctcttcggta ttggagaaca ttttcttctc gataaccgtg cgcagttttt
    12301 catagctggt ccagttcgga ttgcgtccgc tgttattcgc tctggctcgc agtacgaagt
    12361 taacaatctc gttgcggaaa tcttttggat tactgatccc cgccggtttc tcgattttct
    12421 ccagctcggc gttaagagac tcgcggtcaa acaactgccc ggtatccgga tcgcgatact
    12481 cctgatcctg aatccagaaa tccgcgtagg taacataacg gtcgaaaatg ttttgcccat
    12541 attctgaata ggattcaagg taggccgtct ggatctcttt gccgataaac tcggcatatt
    12601 tcgggatcag ataacctttc aggaactcca gatagtgttc agcctgctct tgcgggaact
    12661 gctcgcgctc gatctgctgt tccaggacgt agaacagatg gaccgggttt gccgccactt
    12721 ctacatgatc gaagttgaac acgcgggaga ggatcttaaa cgcaaaacgc gtcgacagac
    12781 cgttcatccc ttcatcgaca ccggcgtagt cacgatattc ctgatacgac ttggctttgg
    12841 gatcagtgtc tttcagactt tcaccatcat aaacgcgcat ctttgaataa atgctggagt
    12901 tttctggctc tttcagacgc gaaagaatag aaaaacgtga cagcgtttcc agcgtgccag
    12961 gggcgcaggg ggcgtgagtc aattcactgt gattaagcaa tttctcgtag attttgatct
    13021 cttcggaaat gcgcaagcaa tacggcacct tcacgatgta aacacggtcg aggaaggctt
    13081 cgttgttttt gttattacgg aaagtgaccc attcggattc gttcgagtgg gcgagaataa
    13141 tcccgttgaa cggcagggcg gagatacctt ccgtcccgtt gtagttacct tcctgggtgg
    13201 cggttaacaa gggatgcagc acttttatcg gtgctttaaa catctcgaca aattccatga
    13261 tcccctgatt ggcgcggcac agcgcaccgg aatagccgta ggcgtccggg tcattctgcg
    13321 cgtagtgttc gagtttacga atatcgactt tccctaccag ggcggagatg tcctggttgt
    13381 tctcatctcc gggttccgtt ttggcgatgg caatttgttg cagaattgac ggccagacct
    13441 tcactacccg gaacttagtg atatcgccac caaattcatg caggcgcttt gccgcccacg
    13501 gcgacatgat ggtgccgaga taacggcgag gaatgccgta ctctttttcc agaatctgcg
    13561 catcttcctg cggattgaaa agacagaacg gatgatcgtt gaccgggcta cgctcaccgt
    13621 tggcgctcaa tacataaatc ggtacgagct gcattaatga tttcagtcgc tcagcaagcg
    13681 atgatttacc cccacccaca ggccccagca gatacaggat ttgtttcttc tcttccagcc
    13741 cctgagccgc gtgtttcaga taagaaacaa tctgttcaat cgcgtcttcc atgccgtaaa
    13801 actcttcaaa cgccggataa cgtgcaatga cccggttaga aaagagtcga gaaagtctgg
    13861 gttcctgggc tgtatcgacc atgacaggct caccaatagc catcaatagc cgctcagccg
    13921 cgttggcata agcactgcga tcttgccgac aagtggtaag aaactcctgc agtgtgaact
    13981 cttcgtcctt ggcagcttca tatcgctggc gatagtgatc gaatatattc atggtatgcc
    14041 gtcctttcgt tttttagcac aggttaagag ccgttcatat gaatagtaga ggctcccgga
    14101 agaggtaaac tgaacgacgt ctcttgctaa aacacagcaa ctctcatgcc attttattga
    14161 cgatgagaac aacgtgcccg gtgatacacc ttcaaaatta agcgtagatg gcaaatggaa
    14221 aacttgcctg cacggctttc ttaatttcaa tggcatatca acaactcatc cataaatttt
    14281 gcataattaa tgtaaataat agggtcgtcg gtaacgctaa attcatttgg ctgtaagcgt
    14341 ggtgtcatct gcgtcaggaa aattaaacag ttactataaa aaatgaaaac gtaaaaaggt
    14401 tgggtttcga tgtattgacg ggtaaacttt gtcgcccgct aaacatttgt ttaaggaatg
    14461 attaattgtg accaaactca aacttctggc acttggagtg cttatcgcaa cgtctgcagg
    14521 cgtagcgcac gctgaaggta aattttccct gggcgcaggc gtaggtgtcg ttgaacaccc
    14581 atataaagat tacgataccg atgtttaccc agtaccggta atcaactatg aaggcgataa
    14641 cttctggttc cgtggcttag gtggtggtta ctacctgtgg aatgacgcga cggacaaact
    14701 ttcaattacc gcttactggt cgccgcttta cttcaaagct aaagacagtg gcgatcacca
    14761 aatgcgtcac ctcgatgacc gtaagagcac catgatggct ggtctgtctt atgctcactt
    14821 tacccagtac ggttacctgc gtaccaccct ggctggcgat accctggata ccagcaacgg
    14881 catcgtctgg gatatggcct ggttgtatcg ttacaccaac ggtggcctga ccgtgactcc
    14941 gggtattggt gtgcagtgga acagcgaaaa ccagaacgaa tactattatg gcgtatcgcg
    15001 caaagagtcc gctcgcagcg gtctgcgtgg ctataacccg aacgacagct ggagccctta
    15061 cctggagctg agcgccagct acaacttcct cggtgactgg agtgtttacg gtaccgcgcg
    15121 ctacacccgt ctgtctgatg aagttactga cagcccaatg gtggataaat cctggactgg
    15181 cctgatttct accgggatca cctacaaatt ctgataatgc atttttccag ggcattttgt
    15241 gcataaaaac agggcgtaat gccctgtttt gcattacggt ggtgcaatac ctgggaggaa
    15301 acaatgcaac aaaaaatgat tcaatttagt ggcgatgtct cactgccagc catagggcag
    15361 ggaacatggt atatgggcga agatgccagt cagcgtaaaa cagaagttgc tgcactacgc
    15421 gcgggcattg aactcggttt aaccctcatt gataccgccg aaatgtatgc cgatggcggt
    15481 gccgaaaaag tggttgggga agcattaatc ggtctgcgtg agaacgtctt tctcgtctct
    15541 aaagtctatc cgtggaatgc tggcgggcaa aaagcgataa atgcatgcga agccagttta
    15601 cgccgtctca atactgatta tctcgatctc tacttattac actggtctgg cagtttcgct
    15661 ctcgaagaga ctgtcgcagc gatggaaaaa ttgatcgccc agggaaaaat ccgccgctgg
    15721 ggcgtttcta accttgatta tgccgatatg caggaactct ggcagctgcc ggggggaaat
    15781 cagtgtgcca ctaatcaggt gctttaccat ctcggttcac gagggattga gtacgatcta
    15841 cttcccaggt gccagaaaca gcagatgccg gtgatggctt acagtccatt agcccaggcc
    15901 gggcggttgc gcaatggact gttaaaaaac gcggtagtca acgaaattgc acatgttcac
    15961 aatatcagtg cggcacaagt attgttggcg tgggtaatca gccatcaggg tgtgatggcg
    16021 attccaaaag cggcaactgt cgcacatgtc caacaaaatg cggctgtgct tgaggttgaa
    16081 ctttcgtcag cggaattagt tatgctggat aaagcgtatc cggcaccaaa aggaaaaact
    16141 gcgctggata tggtgtgatg ttgattcgtg ccggatgcat cgcacccggc acacaatatt
    16201 aacgcaaatt aacgtttagc aacgcgaatg gattgcgcca gatgtgcagg tttctctttg
    16261 gtgacttttt gcgtttctga agcgtaagcc gtttctacac aaacaaaggt cttgtagcca
    16321 tcatccggca tatcgcccat gctaactgaa agcgccggtc ctgggttcca gcctacaacg
    16381 ttcagatgat gctggtggcc tacggcgata atacgattca gcgcttcgtc attaattacg
    16441 ctgcaatctt gcggattcag atacacgcga tcggtacggt cagggaaggt ctgaataccg
    16501 tcggtcagta catcttcttt cgcgtcattt actttatcaa tgaagcgatc gcctagtcca
    16561 ctgacgctta ccttagcgat atcacccacg ttaaagtagg tatgcagagc agaggtagtt
    16621 tcaaattcgc cgtgtgcttc aagatcgatt tcacaggttt tacccacgtg gaaatgcgct
    16681 aacagcgtaa agtcgtgcgg ccagaatttt ttcgtctctt cgctttgtgt caattcaaaa
    16741 gtcagcgcta caccatcagc atcttcacga tgtgatttca gcgtccacgg caggttgcgg
    16801 gcaaaaccgt gcgcaggcag accttgttgt gcagccggac caaaccacgg ccagcaaacc
    16861 ggtatgccac cgcgaatggc gacgccattt ttgaacggtg tgttgttact caaccacaga
    16921 acttcttctt cacccgcagg tttccacgag agaaggtgtg cgccctgtaa tgcaaaagag
    16981 gcttttacct ggggatgatc gaccacaatg aggtccagtt catccagttt acgacgggag
    17041 aggacagggg agatttgttc gatgaccgga agggcaaaaa ttttcttaat catgacgcag
    17101 tcctttaact tcattttatc aggtaaaaaa aagagcgacc gaagtcgctc tttttagatc
    17161 acagtgtcat ctcaacttat ttggagatgt gagcgatcag gtccagaact ttgttggagt
    17221 aaccggtttc gttgtcgtac caggatacca gtttcacgaa gttgtcgttc agagcgatac
    17281 ccgctttagc atcgaacacg gaagtgcaaa cttcgccgtt gaaatcggta gatactacgt
    17341 catcttcggt gtagcccaga acgcctttca tttcgccttc agcagcagct ttaacggcag
    17401 ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac ggtcaggtca actacggata
    17461 cgttcggggt cggaacgcgg aacgccatac cagtcagttt gccattcagt tctggcagta
    17521 ctttacctac agctttagca gcaccggtag aagacgggat gatgttctgg gaagcgccgc
    17581 ggccgccgcg ccagtctttg tgagacgggc catcaacggt tttctgagta gcggtagtag
    17641 cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt atcgttgata actttagcca
    17701 gcggagccag gcagttggtg gtgcaggatg cgttggaaac gatgtcctgg ccagcatatt
    17761 tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc tttagacgga ccagtcataa
    17821 ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt ttcgtcagtc aggaacagac
    17881 cagttgcttc agcgacaacg tcaacaccaa cttcgtccca tttcaggtta gccggatcac
    17941 gttcagcggt aacacggatt tttttaccgt taacgatcag atgaccgtct ttcacttcaa
    18001 cggtaccgtc gaaacggccg tgagtggagt catatttcag catgtatgcc atgtaatcag
    18061 cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga acgtttctga gcagcacgga
    18121 aaacaatgcg accgatacgg ccaaaaccgt tgatacctac tttgatagtc atatattcca
    18181 ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt acaaaaacct cacgcagcgt
    18241 caagcggaat cgtgtcaatc attgcgacaa atcaatcctg tgcctaagca ttacgcgact
    18301 gactcgcctc actcttcctt tgggcttgag accacatggg gtcggcgccc cgaattttaa
    18361 agggcaatta cgataaaaat gtgattttca tcacgattta ggtgcagcca tttcgtcgag
    18421 atcaagttta ggacaaaatt ttgccagttg gtgttaatgt tttgttagaa tcagtcacgt
    18481 aatgtgagca cgattaaagt gagatgtgag caaatggcta ataaaccttc ggcagaagaa
    18541 ctgaaaaaaa atttgtccga gatgcagttc tacgtgacgc agaatcatgg gacagaaccg
    18601 ccatttacgg ggcgtttact gcataacaag cgtgacggcg tatatcactg tttgatctgc
    18661 gatgccccgc tgtttcattc ccaaaccaag tatgattccg gctgtggctg gcccagtttc
    18721 tacgaaccgg tgagtgaaga atctattcgt tatatcaaag acttgtcaca tggaatgcag
    18781 cgcatagaaa ttcgttgcgg taactgcgat gcccatctgg gccatgtctt tcctgacggg
    18841 ccgcagccaa cgggcgaacg ttattgtgtt aactctgctt ctttacgctt cactgatggc
    18901 gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag caacttattc cacaggagcg
    18961 gaaaaattat gaattttgat gacattatca acagcatgac gcctgaggta taccagcgtt
    19021 tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt tacgttgacc gaggaacaaa
    19081 aagagaactg cctgcaactg gtgatgctgt ggcaagcccg ccataatacc gaagcacagc
    19141 atatgacaat tgacacaaat ggtcagatgg tcatgaagag caaacagcag ttaaaagaag
    19201 attttggtat ctcagcaaag cctattgcca tgtttaagta agtggtttat cccctttttg
    19261 ccgcggttat aaaggggata gccaacaatg accccacgac tcatctcaat tatctcgccc
    19321 cgacctggct aagtcccagc ccgatccctt gcagtcgcgt tcacacctga atgacttctc
    19381 ctgaacttaa gtctcaggcc tatttggccg ggaatccctc tcgaatttgc tctttttgcg
    19441 tcccgccttc catcaacctg taacaacccc agccattttc tgcgtaattg tgatccaagt
    19501 tgtaaaacgc agatcattat ctgtgtttta cagatttaat gttgacctca aaaatcatgt
    19561 gggtaagtat gatgaccatc taagcaaaac aaaacacaga ggatgacgca atgaaagcac
    19621 tggctcggtt tggcaaggcc tttggtggct acaagatgat tgatgtccca caacccattt
    19681 gtggcccgga agatgtcgtg attgaaatta aagccgcggc aatctgcggc gcagacatga
    19741 agcactacaa tgtcgatagc ggttcggatg agtttaactc tatccgcggc catgagttcg
    19801 caggttgtat tgcgcaggtt ggtgaaaaag tcaaagactg gaaagtgggg caacgcgtcg
    19861 tgtcggataa cagcggtcac gtttgcggcg tttgcccggc ctgtgaacaa ggtgattttc
    19921 tgtgttgtac agaaaaagta aaccttggtc tggataacaa tacctggggc ggtggttttt
    19981 ccaaatattg ttttgttcct ggtgaaattc tcaaaattca tcgtcatgcg ttgtgggaaa
    20041 tccctgatgg tgttgattat gaggacgcag ccgtacttga ccctatctgt aatgcctata
    20101 aatccatcgc gcagcaatcg aaattccttc caggtcagga tgtggtcgtc atcggcactg
    20161 gcccactcgg gctgttctac gtacaaatgg cgcggattat gggggcggta aatatcgtcg
    20221 tcgttggtct gcaagaagat gtggcggtcc gcttcccggt tgcaaaagaa ctgggtgcga
    20281 cggcagtagt aaatggttct accgaagatg tggtggcgcg ctgccagcaa atttgtggca
    20341 aagacaatct ggggctggtg attgaatgct ccggtgccaa tatcgcattg aaacaagcca
    20401 tcgaaatgct ccgcccgaat ggggaagtag tacgcgttgg aatgggcttc aaacctcttg
    20461 atttctcgat taatgacatt accgcctgga acaaaagcat cattgggcat atggcctatg
    20521 actccacttc atggcgtaac gctatcaggc tattagccag cggcgctatc aaagtcaaac
    20581 cgatgatcac gcatcgtatc ggcctgtcgc aatggcgcga agggtttgat gcgatggtcg
    20641 ataaaactgc aatcaaagtg atcatgactt acgactttga tgaataacta ctctcctgaa
    20701 ggaacgttat cgcatggaac agataacaaa accgcattgt ggtgcacggc tggatcgctt
    20761 accggattgc cgctggcatt cgtcgatgtt tgccatcgtc gcgtttggct tgcttgtctg
    20821 ctggagtaat gccgttggtg gcttgatcct cgcgcagctg aaagcgttgg gctggacaga
    20881 taattccacc actgccacat tctcagcaat cacgaccgcc ggaatgtttc tcggtgcgct
    20941 ggtgggcggc atcatcggtg acaaaaccgg tcgcagaaat gcgtttatcc tctatgaggc
    21001 cattcatatt gcctcgatgg ttgtcggtgc tttctcaccg aatatggatt tccttattgc
    21061 ctgccgtttt gtgatgggcg ttgggctggg agctttactg gttacactgt ttgctggttt
    21121 caccgaatat atgcccggaa gaaatcgcgg aacgtggtcc agtcgggttt cctttattgg
    21181 taactggtca tatccgctct gttcattgat tgcgatggga ctcacgccgc tgattagtgc
    21241 agagtggaac tggcgggtac aactgcttat ccctgcaata ttgtcactta tcgctacggc
    21301 gctggcctgg cgctactttc ctgaatcccc gcgctggctg gaatcgcgcg gacggtatca
    21361 ggaagccgag aaagtcatgc ggagtataga agaaggcgtc atacgccaga cgggtaaacc
    21421 tttgccgccc gtggttattg ctgatgacgg taaagcgcca caagcggtgc cgtattcagc
    21481 cttactgaca ggagtattac tgaaacgcgt gatattaggc tcttgtgtgc tgattgccat
    21541 gaacgttgtg cagtacacac taattaactg gttgccaacg atattcatga cccagggaat
    21601 taatttaaaa gactcgattg ttttaaatac catgagtatg tttggcgcgc catttggtat
    21661 ttttattgcc atgctggtga tggataaaat tccgcgtaaa acaatgggtg tggggctatt
    21721 aatcctgatt gcggtgctcg gatatatcta ttcactgcaa accagtatgt tgctcataac
    21781 gctgattggt ttcttcctga ttactttcgt ctatatgtac gtttgctatg cctcggcagt
    21841 gtatgtccct gagatctggc cgacagaggc caaactccgt ggctccggtc tggcgaatgc
    21901 ggtagggcga atcagtggta ttgccgcacc ttatgccgtt gcagtgctgc tcagtagtta
    21961 tggcgtaacg ggagtcttta ttcttctggg ggctgtttca attattgtcg caattgctat
    22021 cgccaccatt ggaattgaaa ccaaaggtgt ctccgttgaa agtttaagta ttgatgcagt
    22081 agccaataaa taatactggt ggattcaaaa tgaaaaattc aaaagcaata ttgcaggtgc
    22141 cgggcacaat gaaaattatt tcagcagaaa tacctgtgcc taaagaagat gaagttttga
    22201 ttaaagtgga atatgtcggt atttgtggtt cagatgtaca tggttttgaa tcaggcccgt
    22261 ttattccgcc taaagaccca aatcaagaaa ttggcctggg tcatgaatgc gccgggacgg
    22321 ttgtggctgt gggaagccgc gtgcgcaaat ttaaaccggg ggatcgggta aatatcgaac
    22381 ctggcgttcc ttgcggtcac tgtcgttact gtctggaagg caaatataac atctgcccgg
    22441 acgttgattt tatggcgaca caacccaact accgcggcgc attaacgcac tatctgtgtc
    22501 atccggagag ctttacttac aaactgccag acaatatgga cacgatggaa ggggcgctgg
    22561 tggagcctgc cgcagtcggg atgcatgccg cgatgctggc agatgttaaa ccgggtaaga
    22621 agataattat tctgggagcg ggttgtattg gtttgatgac gttgcaagcg tgcaaatgcc
    22681 tgggagcaac ggaaattgcc gtcgttgatg tgctggaaaa acgtctgaca atggcggaac
    22741 agctcggcgc gacagtggtt attaacggcg caaaagaaga cactattgca cgctgtcagc
    22801 aatttaccga agacatgggc gcagatattg tttttgaaac agcgggttct gcggtcaccg
    22861 ttaaacaggc accttatctg gtcatgcgcg gcggtaaaat tatgattgtt ggtactgtac
    22921 ccggcgattc ggcaatcaat ttcctcaaaa tcaatcgcga agtcactatc cagacggtat
    22981 tccgctatgc caatcgttat ccggtcacga ttgaagctat ttcttcaggg cgattcgatg
    23041 tgaaatcgat ggtgacgcat atttacgatt atcgggatgt acaacaggca tttgaagagt
    23101 cagttaacaa caaacgcgac attattaaag gcgttattaa aattagcgat taattaatac
    23161 gggagaacag aacatgctcg cagatatcag atattgggaa aacgatgcca caaataaaca
    23221 ttatgtgcaa ttgcccattt taacgtatgg aatgcagaaa tgttgatggg cgttatcgac
    23281 gcagccgaag aagcgaaatc accggttatt atttcttttg gctcaggttt tgtcggcaac
    23341 acctcatttg aagatttctc tcacatgatg gtatcaatgg cacaaaaagc aacggtgccg
    23401 gtaataactc attgggatca tggtcggagt atggagatta ttcataacgc ctggactcat
    23461 ggtatgaatt cattaatgcg tgatgcttct gcatttgatt tcgaagaaaa tattcgttta
    23521 accaaagagg ctgttgattt cttccatccg ctgggtattc cggtagaggc ggaattaggg
    23581 catgtcggta atgaaaccgt ttatgaggaa gcgctggcgg gctatcacta taccgatcct
    23641 gaccaggcgg ctgaatttgt tgaacgtacg ggctgtgact cattggctgt cgccatcggc
    23701 aaccagcatg gggtttatac gtcagagcca caattgaact ttgaggtcgt caaacgcgta
    23761 cgcgatgctg tttctgttcc gctggttttg catggcgcat cggggatcag tgatgccgac
    23821 attaaaactg caatttcgtt gggtattgcg aaaatcaaca ttcaggtaat gactccaact
    23881 tattgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag ctccaccgat
    23941 tttgagaacg acagcgactt ccgtcccag
//
LOCUS       DAZGJT010000073        23738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZGJT010000073 DAZGJT010000000
VERSION     DAZGJT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.073"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..72
                     /locus_tag="ACHKFK_003158"
     CDS             <1..72
                     /locus_tag="ACHKFK_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9053436.1"
                     /translation="SFSKSVELHDKVIGHYLNIKHYQ"
     gene            complement(586..987)
                     /gene="cadC"
                     /locus_tag="ACHKFK_003159"
                     /pseudo
     CDS             complement(586..987)
                     /gene="cadC"
                     /locus_tag="ACHKFK_003159"
                     /inference="COORDINATES: protein motif:HMM:NF037258.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            1130..1255
                     /gene="laoB"
                     /locus_tag="ACHKFK_003160"
     CDS             1130..1255
                     /gene="laoB"
                     /locus_tag="ACHKFK_003160"
                     /inference="COORDINATES: protein motif:HMM:NF038068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arginine responsive protein LaoB"
                     /protein_id="HFO9053437.1"
                     /translation="MLRLWEGESVAGTASGIGGGEDSIISSPSSSLYQITGTINL"
     gene            complement(1258..1545)
                     /locus_tag="ACHKFK_003161"
                     /pseudo
     CDS             complement(1258..1545)
                     /locus_tag="ACHKFK_003161"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            complement(1805..1916)
                     /locus_tag="ACHKFK_003162"
                     /pseudo
     CDS             complement(1805..1916)
                     /locus_tag="ACHKFK_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2162..2237)
                     /locus_tag="ACHKFK_003163"
     tRNA            complement(2162..2237)
                     /locus_tag="ACHKFK_003163"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2202..2204),aa:Phe,seq:gaa)
     gene            complement(2344..2919)
                     /gene="yjdC"
                     /locus_tag="ACHKFK_003164"
     CDS             complement(2344..2919)
                     /gene="yjdC"
                     /locus_tag="ACHKFK_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9053438.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(2956..4653)
                     /gene="dsbD"
                     /locus_tag="ACHKFK_003165"
     CDS             complement(2956..4653)
                     /gene="dsbD"
                     /locus_tag="ACHKFK_003165"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO9053439.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNEASQPVSVPQQEQPT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFIMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGETHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTVNDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(4629..4967)
                     /gene="cutA"
                     /locus_tag="ACHKFK_003166"
     CDS             complement(4629..4967)
                     /gene="cutA"
                     /locus_tag="ACHKFK_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO9053440.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(5083..6384)
                     /gene="dcuA"
                     /locus_tag="ACHKFK_003167"
     CDS             complement(5083..6384)
                     /gene="dcuA"
                     /locus_tag="ACHKFK_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO9053441.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(6502..7938)
                     /gene="aspA"
                     /locus_tag="ACHKFK_003168"
     CDS             complement(6502..7938)
                     /gene="aspA"
                     /locus_tag="ACHKFK_003168"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO9053442.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            8275..8751
                     /gene="fxsA"
                     /locus_tag="ACHKFK_003169"
     CDS             8275..8751
                     /gene="fxsA"
                     /locus_tag="ACHKFK_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO9053443.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            complement(8767..10023)
                     /gene="yjeH"
                     /locus_tag="ACHKFK_003170"
     CDS             complement(8767..10023)
                     /gene="yjeH"
                     /locus_tag="ACHKFK_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO9053444.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSGQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPGNGITT"
     gene            10299..10592
                     /gene="groS"
                     /locus_tag="ACHKFK_003171"
     CDS             10299..10592
                     /gene="groS"
                     /locus_tag="ACHKFK_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO9053445.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            10636..12282
                     /gene="groL"
                     /locus_tag="ACHKFK_003172"
     CDS             10636..12282
                     /gene="groL"
                     /locus_tag="ACHKFK_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO9053446.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            12420..12773
                     /gene="yjeI"
                     /locus_tag="ACHKFK_003173"
     CDS             12420..12773
                     /gene="yjeI"
                     /locus_tag="ACHKFK_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO9053447.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(12823..13692)
                     /gene="yjeJ"
                     /locus_tag="ACHKFK_003174"
     CDS             complement(12823..13692)
                     /gene="yjeJ"
                     /locus_tag="ACHKFK_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO9053448.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDDQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFNHYLAVLYRFKDESGNEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(13927..14955)
                     /gene="epmB"
                     /locus_tag="ACHKFK_003175"
     CDS             complement(13927..14955)
                     /gene="epmB"
                     /locus_tag="ACHKFK_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO9053449.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            14997..15563
                     /gene="efp"
                     /locus_tag="ACHKFK_003176"
     CDS             14997..15563
                     /gene="efp"
                     /locus_tag="ACHKFK_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO9053450.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            15615..15740
                     /gene="ecnA"
                     /locus_tag="ACHKFK_003177"
     CDS             15615..15740
                     /gene="ecnA"
                     /locus_tag="ACHKFK_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO9053451.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            15851..15997
                     /gene="ecnB"
                     /locus_tag="ACHKFK_003178"
     CDS             15851..15997
                     /gene="ecnB"
                     /locus_tag="ACHKFK_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9053452.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            16173..16490
                     /gene="sugE"
                     /locus_tag="ACHKFK_003179"
     CDS             16173..16490
                     /gene="sugE"
                     /locus_tag="ACHKFK_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9053453.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(16487..17020)
                     /gene="blc"
                     /locus_tag="ACHKFK_003180"
     CDS             complement(16487..17020)
                     /gene="blc"
                     /locus_tag="ACHKFK_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9053454.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(17109..18242)
                     /gene="ampC"
                     /locus_tag="ACHKFK_003181"
     CDS             complement(17109..18242)
                     /gene="ampC"
                     /locus_tag="ACHKFK_003181"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9053455.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(18305..18664)
                     /gene="frdD"
                     /locus_tag="ACHKFK_003182"
     CDS             complement(18305..18664)
                     /gene="frdD"
                     /locus_tag="ACHKFK_003182"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9053456.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            complement(18675..19070)
                     /gene="frdC"
                     /locus_tag="ACHKFK_003183"
     CDS             complement(18675..19070)
                     /gene="frdC"
                     /locus_tag="ACHKFK_003183"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9053457.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIMIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(19081..19815)
                     /gene="frdB"
                     /locus_tag="ACHKFK_003184"
     CDS             complement(19081..19815)
                     /gene="frdB"
                     /locus_tag="ACHKFK_003184"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9053458.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(19808..21616)
                     /gene="frdA"
                     /locus_tag="ACHKFK_003185"
     CDS             complement(19808..21616)
                     /gene="frdA"
                     /locus_tag="ACHKFK_003185"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9053459.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            21941..22918
                     /gene="epmA"
                     /locus_tag="ACHKFK_003186"
     CDS             21941..22918
                     /gene="epmA"
                     /locus_tag="ACHKFK_003186"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9053460.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            23137..>23738
                     /locus_tag="ACHKFK_003187"
     CDS             23137..>23738
                     /locus_tag="ACHKFK_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9053461.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNP"
BASE COUNT         5896 a         5821 c         6038 g         5983 t
ORIGIN      
        1 tcgttctcaa aatcggtgga gctgcatgac aaagtcatcg ggcattatct gaacataaaa
       61 cactatcaat aagttggagt cattaccaag ggtgtttgcc cctgggcgta aattaaaggc
      121 ggtgagatat gcatcagctg cttcccggtt catccccttc atttcataaa ccttgccaag
      181 caacacataa tttagccagg acatttcaag atcaatgccg gtatttatcg cctgataaga
      241 ctcatctgtt ttacctttta ccagggcact gaccgctttt atttgatata taatggacag
      301 gttgttcaat tccggcagtg taacaatgtt atctatttct gtgttcagtg ctgctaattg
      361 tttttcgtct aaaggatgtt gagaatggcg cacgatatca actaatgctt tttctgctct
      421 cgcgtaggta aattctgggg atgattgaac aatctcacct aataattcac tggcacgatc
      481 caatgattta tcatcaccat gcagtaaata atcatgtgcc tgataaaaat tagttaataa
      541 cgcaccacga tgcggcaaaa tttgctggag catctcctgc attcgttgtg gccacggttg
      601 gtttaacgct tttgataaac tctggagcaa atcattttga atcgccagct gattaccgtt
      661 agtgatgaca taacgtttat ccagcatggt tgaaccatct gcattgtcta ccaattttac
      721 cgacataaag cattgttgag cacggtattg gcgctgatta acaaacgcaa tagataatgt
      781 tttaccggaa ctgctcggtt catcaatgtt gtagttgatt ttgtcatgca ccataaaggt
      841 ggagaaggtg ttaagtgatg tcgccaccaa atcacccacg cctatcgcgt aagagagctg
      901 atacggagaa ctccagctgt tacaactctt attaaccata ttaatgtcaa tatcgcgtgg
      961 attgagcaaa atgcgcgatt tactcatagg aagacgtgtt tcaagatttg aaaacgctac
     1021 cagtgcgaca cagataccta acgacaacag gaaaaaaaac catacccaaa aggtagtgaa
     1081 tcgtttgctt ttaactgggg attgttcagg tggcgttgtg gtgttttgaa tgttaagact
     1141 gtgggaggga gaatctgtgg caggaaccgc ctctggtata gggggaggcg aagatagcat
     1201 tatttcctct ccctcttctt cgctgtacca gataaccggc accattaatt tatagccgcg
     1261 ctttggtaca gtagcgatat agacaggact atcttcatca ttatctttta atgacttacg
     1321 tagttctgag atactctgcg tcacaacgtg attggtgaca atacttctct tccagacatt
     1381 atcgataagt tcatccctgc taagtacttc gccactgtgt tgagcaaaga aaaccagaag
     1441 atcgattaat ctcggctcaa gggtaagttg acgcccattg cggctaattt ggtttatgga
     1501 cggagtaaca agccattcgc caacgcgaac tacaggttgt tgcataatag aaactcattc
     1561 gaaaagggaa tgatgcaatg ataattgcca taacctattt tttccatcta tagatgggtt
     1621 tatttacata ttattggtga atgtaggacg ttatttttac ccgccataaa cttcttgatt
     1681 acatagtatt acgaaaggat tttactgaga accagaagta attttcctta ccatcaaaat
     1741 tcatcatctt tgccaaagaa aaatgttcag aaaataatcc atgaaaaatt gtccggagcg
     1801 cttactattt taatggattg ttagtctttg catgagcaaa cggactgata catttctctt
     1861 tgttctcatt cagaaaatct catcagttgg cgttctgccc gatgttgtgc tttatgggtg
     1921 cgttacgctc ctgatgacgt catttgacgt tcaacagcat cacggggccg cacgacattt
     1981 cacgtcagtc agcgctatag ctcagaaaca aattttcccg aattgggata tgcccgcaaa
     2041 ttgctggtga tgtggggaga atctggttga gttcggtaga attgatttgg cggcaggacg
     2101 cttaaatcgt ggcgtcctga aacgaaaaac ggacctccgt ggaggtccgt ttatatgaat
     2161 ttggtgcccg gactcggaat cgaaccaagg acacggggat tttcaatccc ctgctctacc
     2221 gactgagcta tccgggcaac ggggcgcatt aaaccgtaat ctgcacatct cgtcaaccta
     2281 atttcagtaa aagcgattca actgcttaag attgcggcaa atcgcttctt ttctgtgttt
     2341 ctgtcaggtc agtgcaccac cctgacggca gcgggcgaag cgcaggatat cttccgccag
     2401 ccgatgtgcg gtgtcgacat ccgcctggct acgattcacc agcattcggc ttaaacagcc
     2461 ttccagcacc agttccatct gctttgctac catcgccgga tcgtcaactt ccagcgtggt
     2521 taacagttcg tgggtgaaat cgtaggccgc gcttttttgc tgatcggcca gttgatgaat
     2581 agggtggcca ggatcggggt aaaacgtaca ggcagcgata aacagacagc ccggatagcg
     2641 gttgttttta acgcactccg ataacgcctg ataacgtgcc agcaactttt gttcggcggt
     2701 ttgcgtttcg tccagcatca gctgacgacg ccagacatct atctgttggc taagataacg
     2761 cagcgcatcg tagaggattg cctctttgtc tggccagaag cggcgtagct cgtccagtgg
     2821 ataatccaca cgttcagcaa ccatctccag cgtggtgttg gcaatccctt gtaattctaa
     2881 taatttcagg gcttctccca gtacatcttc acgttgcacg ctattttcct ccgtctttcc
     2941 cactgcaagt gtcgttcacg gttggcgatc gcgcaaatgt gcgctgaagg tttcagcatc
     3001 cataaagccc gtgacgcgtg cttgtggatg ctcctggcct tgtccgtcaa aaaagagaat
     3061 tgtcggtagg ccaaggacat taagatgctt taacagcgcc acatcttgtg cgtcgttgac
     3121 agtgacgttg gcctgaagta agaccgtatc tgctaacgct ttttgcacct gcgggtcgct
     3181 gaaggtgtat ttctcaaact ctttacaggc gacgcaccag tcggcataga gatctaacat
     3241 cactggtttg cctttggctt caacgagcgc ctgatttaac tcatctactg ttttgatttg
     3301 tgtaaagttg agatgcgtct gagtttgcgc ggtatgtgtt tcaccaaatg cccaatcctg
     3361 aagtgggcgc acgctaacca atgccgctgc cagcaggatt atttgcacca cgcgcatcca
     3421 gccgcgtttg gcctgtaggc tggtgataaa ggcccagcca aagaatgcga caccaagcgc
     3481 cgaccacaag cgtaatcccc atatatcacc aatcactcgc tccagcagga agaccggcag
     3541 tgcgaggatc acaaaaccaa acgcggtttt gacttgttcc atccacgggc cgcttttcgg
     3601 cagcaagcgg ttaccaaaga cggtaattag catcagcggc aggcccatgc ccagcgcata
     3661 aagataaagc gtgccgccgc ccagccacat gttcccgctt tgggcgatat acagcagaat
     3721 cgcgctaagc ggtgcagtgg tgcatggtga acagatcagt ccggcaatcg cccccataat
     3781 aaacacaccg ccaggtgagc cgccctgttg gcgattgctc atcagcgtga gacgtgtttg
     3841 cagcgaagag gggagttgca gagtaaacaa gccaaacatt gacatcgcca gcaaggtaaa
     3901 gacgatggcg aggccaatga gcacgtatgg gtgctgtagc gccgcctgga actgtaaccc
     3961 tgcggcggca accaccagac ccagcgccgt gtaagtcagc gccatcccct gcacataaat
     4021 aaaggtcagc aacaatgctc tggcagtgga aagccgctgt ttaccgccca gcacgatgcc
     4081 agaaatcagt gggtacattg gcagcacgca tggcgtaaag gcgataccaa taccgatcaa
     4141 caacgcccag agcgcggaaa agggcaattg cgcggtgggc tgctcttgct gcggaacaga
     4201 cacaggctgt gacgcttcgt tgttagcgac cacttcgctt aacggaacgg ttttggtttc
     4261 tggcggataa cagaaaccgg catcagcaca gccctggtag gtgacagtta acgttgctcc
     4321 cgcactcgcc tggttgatgg ttacgggaag cgtcagccga tcgcggtaaa tctcgctttt
     4381 gccgtaaaac tcatcttcat gccagacgcc ttgcggcagc tgcacgtcag caattttcgc
     4441 gtgttccggc gtaatgcgga tctgtttacg gtagaggtag taaccgtctt tgatctgcca
     4501 ggtcagattc agatcatgtt gattttgctg aaaatcaaaa gcaaaggctt gatccgcggg
     4561 gacaaattgt gaacgtcccg gcgcgtcgaa taatccggca aaaacggaag tgctgcaaag
     4621 tagcaggatc agcgtaaaga tgcgttgagc catgagaggt aatctgtgtc tccgtgtgta
     4681 acaggtaaaa ccagaagttc cggggtttga tatggatgat gagacttcag gcattccagc
     4741 agtgcctgct ggtgagatac ggtagttttt aaaatcatct gcacttcgta ttcttgctcc
     4801 agcttacctt cccagtaata gagagaggta gcgccgggga tcaaggtcgc gcaggccgcc
     4861 agtttttccg ccagcacttt ggcggctaaa tcctgggctg tcgcttcatc cggtgccgta
     4921 catagcacca cgacagacgt ggtattcgaa cttttttcat caagcataaa cacctcgcaa
     4981 gaacagatgg aaccgcaaga gagaggtcac tatacaacgg acggggaaag gatgttagtc
     5041 ggcgggaaag aaagcggggc gtgaacgccc cgcgatgggt cattacagca tgaagctacc
     5101 cagcacgaag ccgaagcaaa cggccagggc aacacccaga gtacccggga tgaagaacgg
     5161 atggttgaag acgaatttac cgatacgggt agtacccgta tcatccatct gtaccgcagc
     5221 aaccagcgtc gggtaggtcg gcagaataaa cagaccagac accgcagcaa aagaagcaac
     5281 agcggtcagc ggagaaacgt tcagtgccag agccatcggc atcagtgctt ttgcggttgc
     5341 agcctgagag tacagcagag cagaagcaaa gaagaagatg acggccagca gccacggatg
     5401 accctgaatc acttcaccag cggtatcttt gatccagtcg atgttgttgg aaacgaaagt
     5461 atcgcccagc cacgcaacac ccaggataca aatacaggcg ctcatacctg ctttgaaggt
     5521 gctggagttg agaatgttgt cggtatcgac tttacagata acggtggtca gagttgcaac
     5581 gctgagcatg atgatcagga ttgcgttggt ggtgttcatc agtggttttt caaccagacc
     5641 catgcttggg ctgttgatga ttgcatagat aaccacgcca actacgccca gcaggaacag
     5701 ccagacggac gtttttgcac cggatttgat ttcaatctgc ttttcaccgc gcagttcaac
     5761 caggccctct tccagacgct tgcgataaat cggatcgtca gagagtttgg agttgaacag
     5821 catagtgacc aggaaggaca tcaccagaac cgccagcagg gtggacggga tgaccacgga
     5881 gagcagatgg aggtagctga tgccatgacc ttccatcacg gaagacatgt aaaccactgc
     5941 cgctgagatt ggcgatgcgg tgatcgcaat ctgcgcggat accactgcag tagacagcgg
     6001 acggcaaggt ttaacgcctt gttccttcgc aacttcagcg ataactggca gtgtcgccag
     6061 agagatgttg ccagtaccag caaagatagt caggaaatag gtcacgatcg gtgcgaggat
     6121 cgtgatgtat ttcgggttac ggcgcagcag cttttctgtc tgatgaacca gatagtccag
     6181 accgccagca acctgcatgg cagaaatagc ggcgataacc gccatgataa tggaaatgac
     6241 atcgaatggg atgttaccgg gtttaacgcc aatagcggca agaaccagca cccccaatcc
     6301 gcctgcaaaa ccaataccta ttccccccaa tctggcgccc aagaagatcg ccagcaaaac
     6361 tatgatgagt tctacaacta gcatattagc cttccttgtt ttttaacaag ttgatattaa
     6421 attgttattt ttaagttact gctcacaaga aaaaaggcac gccatctgac gtgccttctt
     6481 tatttgtact accctgtacg attactgttc gctttcatca gtatagcgtt ttgctttgta
     6541 agccgggtgc atcagattct gtacggagaa aatatcgtca agttccgctt cagtcaacag
     6601 accgcgttcc agaacgactt cacgtacact cttaccggtt tcggcacaga ttttacccac
     6661 gatgtcaccg ttgtggtgac cgatgaacgg gttcaggtaa gtaacgatac cgatagagtt
     6721 gtaaacgtaa ccttcgcaca cttctttgtt agcagtgatg ccgttaatgc atttttccag
     6781 caggttgtag caagcgttgg tcagaatgtg aacggattcg aacatggcct ggccaatgac
     6841 tggctccata acgttcaact gcagctgacc tgcttctgct gccatggtaa cagtggtgtc
     6901 gttaccgatg actttgaagc atacctggtt aaccacttcc ggaacaaccg ggtttacttt
     6961 agctggcatg atggaagagc ctgcctgcag ttccggcagg ttgatctcgt tcaggccggc
     7021 acgtggacca gaagagagca agcgcaggtc gttacagatt ttggacatct tcacagccag
     7081 gcgtttcagc gcgccgtgaa ccataacata agcgccgcag tcggaggtcg cttcgatcag
     7141 gtcttcagcc ggtacgcatg ggaagccagt gacttcagcc agttttttca ctgccagcgg
     7201 agagtactct ttcggcgtgt tcagaccagt accgattgct gttgcgccaa ggttaacttc
     7261 cagcagcagt tcagcggtac gttggatatt tttcacttct tctttcagca agatgctgaa
     7321 ggcgcggaat tcctgaccga gggtcatcgg tactgcgtcc tgcagctggg tacgacccat
     7381 tttcaggatg tcctggaatt cgacagcttt acgttcaaag ccttcacgca gttggttaat
     7441 cgcatctacc agtttaatca gggaagagta aactgcgata cggaaaccgg tcgggtaggc
     7501 gtcgttagtg gactgacatt tgttaacatg gtcgttcggg ttcaggtact gatattcacc
     7561 tttctggtga cccatcagtt ccagaccgat attggccagc acttcgttgg tgttcatgtt
     7621 tacggaagta cctgcgccgc cctggtagac gtctaccggg aactgatcca tgcattttcc
     7681 gttgttcagg acttcatcac atgcggcaat gatggcattc gctacacttt taggaatggt
     7741 ttgcagctct ttgtttgcca tagctgcggc ttttttaacc attaccatac cgcgaacaaa
     7801 ttcaggaata tcactgattt tgttgttgct gatatagaag ttttcaatcg ctctcagagt
     7861 gtgaacacca tagtaggcat cagctggaac ttccctggta cccaacagat cttcttcgat
     7921 acgaatgttg tttgacatgt gaaccttctt tttcaagctg ccaatgattt gctttaaaca
     7981 cacagaatat atgtggtttc gaatgttttt cgaccgacga ttatcccctg catcgaccga
     8041 atacccgaga tcatatgctg cttgaggatt tctaccgtaa cctggatcac tttaagtgtc
     8101 ggtttttacc ccttaattat taatttgtga aatggatcac cgctttggga ttacgaccaa
     8161 aaatagttgc gcaaacatct tgaaattttg ctaatgacca caatataagc taaacgcgat
     8221 tcgcaaccca ttcaggtagc cgggtttaac cggctgctat tacaggagaa acctttgcgc
     8281 tggttacctt ttattgccat tttcctttat gtctatattg agatttcaat ctttattcag
     8341 gttgctcatg tattgggggt attgctgacc ctcgtgctgg ttatattcac gtcagttatc
     8401 ggtatgtcac tggtacgtaa ccagggtttt aagaatttcg tgctgatgca gcaaaaaatg
     8461 gcggcgggtg aaaacccagc ggcggagatg attaaaagtg tttcgctgat cattgccggt
     8521 ttgctgcttt tattaccggg ctttttcacc gacttcctcg gtcttctgct tttattgccg
     8581 ccggtgcaaa agcatctgac agtgaagttg atgcctcatc tgcgtttttc tcgcatgcca
     8641 ggcggcggtt ttagcgccgg gaccggtggc ggtaatactt ttgatggtga gtaccagcga
     8701 aaggatgatg accgcgaccg ccttgatcat aaagacgatc gccaggatta atgtcgaaac
     8761 gccggattat gtggttatgc catttcccgg cgtttttcgt tttggcagaa acagccataa
     8821 ccccgccagc atgatcagcg cgtagagact tttccagccg accattgcca gtaacagaac
     8881 gcataatagc ccgccaacca ctgccagtag tcgataacgt ccttgcaata atttacagcc
     8941 tgccagcatg cataacagat aaatcataat aaagatgcca ttggcataaa taataagagc
     9001 gtccagattg atctctaaag catgaatcac caacgtgctc accacgcagc agccgagcac
     9061 cgcattgagg gcattattcg gaatatggcg agaagagagg cgtgccaggt agtggtcagg
     9121 attatgttgc gcctgcgacc agaccaggcg ggcgaagctc tgtatataaa tgttgagact
     9181 ggcaaagcag gccagatagc caattacgca ggcaatccat aacgctccta caccgaacaa
     9241 ctgcactaca attttgggaa gcgatgctgc cgccgccatt tgttcaccat aggcgtcgaa
     9301 gtgtaagacg actaccgtac agccccaata gactaatcct gccagcagca ggccaatcat
     9361 caaagcacga ggaaaatcac gctctggatt tttaaattcc gaggcaagat gggcaaatgc
     9421 ttccagaccg acaaaacacc agaacatcac tgataacgca gcgaataacc cggtaagttc
     9481 gatatttcct ggcgcaggga aggggatatt cgcaggtttg atatcgcccg cccaccagat
     9541 agcgacaatc agtgcgacga taagcccggc aataactgtt tgtagattag cactggaact
     9601 ggcacctcga gtaccgatat accacaccag cgccagcgtg ccgagttctg ccaacaacag
     9661 ttgcccgcta tgccagccaa acattgcctg gccgaatccg gcagcaattt gtagcgcggc
     9721 aggcaaaccc acgggaatga ccgataaaaa caaccagccg gtgactcgct caagccgcga
     9781 accaaacgcc ataccgacga agtgcgcgac gccgcctgcg ctgggatagt ggcgacccag
     9841 aatcgcaaac acaatcgcaa tcgggaacac taagataatc aaaacgggcc acgcccacag
     9901 gctgttattg cctgctacta gcgcagctaa cgcaggaacg gcaaacacgc cagtgcctaa
     9961 taatgacgtc gatagcaggc cgatgccctg ggccagcccc agttcttgtt tgagtccact
    10021 catgggttga tgtccgattg cgcccaaatt ttgggcaact gcgtagattt tcgatggtag
    10081 caaaatcaga ttcgcttatg acggtgatga agaaatagcg atgaaatgtg aggtgaatca
    10141 gggttttcac ccgattttgt gctgatcaga attttttttc tttttccccc ttgaaggggc
    10201 gaagcctcat ccccatttct ctggtcacca gccgggaaac cacgtaagct ccggcgtcac
    10261 ccataacaga tacggacttt ctcaaaggag agttatcaat gaatattcgt ccattgcatg
    10321 atcgcgtgat cgtcaagcgt aaagaagttg aaactaaatc tgctggcggc atcgttctga
    10381 ccggctctgc agcggctaaa tccacccgcg gcgaagtgct ggctgtcggc aatggccgta
    10441 tccttgaaaa tggcgaagtg aagccgctgg atgtgaaagt tggcgacatc gttattttca
    10501 acgatggcta cggtgtgaaa tctgagaaga tcgacaatga agaagtgttg atcatgtccg
    10561 aaagcgacat tctggcaatt gttgaagcgt aatccgcgca cgacactgaa catacgaatt
    10621 taaggaataa agataatggc agctaaagac gtaaaattcg gtaacgacgc tcgtgtgaaa
    10681 atgctgcgcg gcgtaaacgt actggcagat gcagtgaaag ttaccctcgg tccgaaaggc
    10741 cgtaacgtag ttctggataa atctttcggt gcaccgacca tcaccaaaga tggtgtttcc
    10801 gttgctcgtg aaatcgaact ggaagacaag ttcgaaaaca tgggtgcgca gatggtgaaa
    10861 gaagttgcct ctaaagcgaa cgacgctgca ggcgacggta ccaccactgc aaccgtactg
    10921 gctcaggcta tcatcactga gggtctgaaa gctgttgctg cgggcatgaa cccgatggac
    10981 ctgaaacgtg gtatcgacaa agcagttacc gctgcagttg aagaactgaa agcgctgtcc
    11041 gtaccgtgct ctgactctaa agcgattgct caggttggta ccatctctgc taactccgac
    11101 gaaaccgtag gtaaactgat cgctgaagcg atggacaaag tcggtaaaga aggcgttatc
    11161 accgttgaag acggtaccgg tctgcaggac gaactggacg tggttgaagg tatgcagttc
    11221 gaccgtggct acctgtctcc ttacttcatc aacaagccgg aaactggcgc agtagaactg
    11281 gaaagcccgt tcatcctgtt ggctgacaag aaaatctcca acatccgcga aatgctgccg
    11341 gttctggaag ctgttgccaa agcaggtaaa ccgctgctga tcatcgctga agatgttgaa
    11401 ggcgaagcac tggcaactct ggttgttaac accatgcgtg gcatcgtgaa agttgctgcg
    11461 gttaaagctc cgggcttcgg cgatcgtcgt aaagctatgc tgcaggatat cgcaaccctg
    11521 actggcggta ccgtaatctc tgaagagatc ggtatggagc tggaaaaagc aaccctggaa
    11581 gacctgggtc aggctaaacg tgttgtgatc aacaaagaca ccaccaccat catcgatggc
    11641 gtgggtgaag aagctgcaat ccagggccgt gttgctcaga tccgtcagca gattgaagaa
    11701 gcaacttctg actacgaccg tgaaaaactg caggagcgcg tagcgaaact ggcaggcggc
    11761 gttgcagtta tcaaagttgg tgctgctacc gaagttgaaa tgaaagagaa aaaagcacgc
    11821 gttgaagacg ccctgcacgc gacccgtgct gcggtagaag aaggcgtggt tgctggtggt
    11881 ggtgttgcgc tgatccgcgt agcgtctaaa ctggctgacc tgcgtggtca gaacgaagac
    11941 cagaacgtgg gtatcaaagt tgcactgcgt gcaatggaag ctccgctgcg tcagatcgtc
    12001 ctgaactgcg gcgaagaacc gtctgttgtt gctaacaccg ttaaaggcgg cgacggcaac
    12061 tacggttaca acgcagcaac tgaagaatac ggcaacatga tcgatatggg tatcctggat
    12121 ccaaccaaag taacccgttc tgctctgcag tacgcggctt ctgtggctgg cctgatgatc
    12181 accaccgagt gcatggttac cgacctgccg aaaaacgatg cagctgactt aggcgctgct
    12241 ggcggtatgg gcggcatggg tggcatgggc ggcatgatgt aattgccctg cacctcgcag
    12301 aaataaacaa acccccgggc agaaatgtct gggggttttt cttttggtca tctttctagt
    12361 ataagattca gacacggacg acgcgagtgg cgtccagctc attgattatg gggaataaca
    12421 tgcacgtaaa atacttagca gggattgtcg gtgccgcgct actgatggcg ggttgtagct
    12481 ccagcaacga attgagtgct gccggtcaga gtgtacgcat tgtggacgag caaccaggtg
    12541 cagagtgcca gctgattggt actgcgacag gtaagcaaag caactggctt tccgggcaac
    12601 acggagaaga aggcggttct atgcgcggcg cagcaaacga tctgcgcaac caggcggctg
    12661 caatgggcgg taacgtgatt tatggcatca gcagcccgtc gcagggaatg ttgtccagtt
    12721 ttgtcccgac ggatagccag attatcggtc aggtttataa gtgcccgaac tgatgtgatc
    12781 cagaggctgg atacttccag cctcgtagta aaacagatga gattatttcg cctgtggtgc
    12841 agttttgctg gtggattgaa cacgtaagtg atgcagcgcg cgtaagcatt gatcctgggt
    12901 tagcggttgg gcgttatttg ccgctacggt gcgaacatag acctgacatg gcacacccgc
    12961 gagttttttc agtcgggggc tgaaatcaag cattcggcga gtgatcgcct ccacttcttt
    13021 acccggcgtt gcttcacgcg ttttcaccac tgcgccgcta aattgttcat tgccactttc
    13081 atctttaaag cggtaaagca ccgccagata atggttgaac aagttatgtt tccattccgg
    13141 ttgggaatag gtgtcatatt ccagattgcc attttcctgg cagtcaacgt cgtataacgg
    13201 caggaaatct aacagtgagg taatacgtaa tgccagttca cgcatttccg cattattaat
    13261 tgcatgaatg attgctcgcg ccagcatttc aatctgcaac tcattgacga ctaactcaca
    13321 tttgctaccg tcatgtagcg ttaatacgaa ggtaagattt tcgccctgat catcggtaag
    13381 ttccagcgtg ttaacccgac gattaatatc ggcattttta agctcatcaa ccagaatttc
    13441 tgggatattt tctgccattt tcttaataac tttatcgcga gcttgttcat actgaaggcg
    13501 tgcagcagca tccagtttat gcttttgatg cagacgactt tccagcgcaa tcagaaggtc
    13561 gcgcaattcc atggcagaca taaagaacaa cgattcttta ttgcgaggtt ctttaatttt
    13621 cagcgccagt gcaatgaaat tattactctt gcgaataaca ccggtattaa cacccttgat
    13681 gcttatggcc atgtaggttc tccctaacca tttctcaatt aaataattaa ttttagttta
    13741 taagccagat aaatgggctt ggtagtaata gttgttaaaa taacataaat agccgtgctc
    13801 actctatata aacagaaaag aaaggttaat tattggtgtt ggttatataa agagtaacaa
    13861 cagcaatgta tatgaataat taaaggttat gcaacgggca aagattaaac ttccctgttt
    13921 aatcagttac tgctggcgta gctggagatc cagcggcgtt ttgctgggtt cgccgccaat
    13981 ttctcgcgcc agtttaggca ccagatatcc cgacaccaat gtcagcaact cacgcataat
    14041 ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg ccctgtactt tatcgagcac
    14101 atgcaggtaa tacggcatca cgcccgcatc gaacaacgca ttactcaggt ttgccagcgt
    14161 ttgcgcgtta tcgttcacac cacgtaacag aacgctctgg ttcagcaaag taacgcccac
    14221 ccggcgcaac ttagccatcg cctgacggaa tgtttcatct acctcattgg catggttgat
    14281 gtgattcacc agcaagattt gcagcgtaga atgagaaaag cgttcaacca gcgcatctgt
    14341 gatgcgtgcc gggatcacaa tcggcagacg gctgtgaatc cgcagacgtt tgatatgcgg
    14401 gatggcttcc agttgtgtga gcaaccagtc cagctcgtga tctttcgcca tcagcggatc
    14461 gccgccggag aaaatcatct cgtccagttc cggatgcgca gcaacatact caagtgcagt
    14521 ctgccagtta cgcttgttgc cctgattttc ggcatagggg aagtggcgac ggaagcaata
    14581 gcggcaattt accgcgcagc cgcctttgac cagcaaaagc gcccggttgt ggtatttatg
    14641 caacaaacca ggcactacgc tatgctgttc ttccagcggg tcggtggaga atccgggcgc
    14701 gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa agaggatcgt ccggattgcc
    14761 tttctccatg cgatcgataa atgagcgggg cacacgcagg gcaaacagct ttttggcgct
    14821 gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa agacgcagaa gttcatcagg
    14881 atcggtcaca acatcggcaa gttgcgttaa ccaatcttct ctggatgggg tatttagggt
    14941 tacaatatgc gccattttgt ggcttagcta ccaattaaca aatttcagag ggccttatgg
    15001 caacgtacta tagcaacgat tttcgtgctg gtcttaaaat catgttagac ggcgaacctt
    15061 acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg ccaggcattt gctcgcgtta
    15121 aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac cttcaaatct actgattctg
    15181 ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct gtacaacgac ggtgagttct
    15241 ggcacttcat gaacaacgaa actttcgagc agctgtctgc tgacgcaaaa gcaattggcg
    15301 acaacgctaa atggttgctg gatcaggcag agtgtatcgt aactctgtgg aatggtcagc
    15361 cgatctccgt tactccgccg aacttcgttg aactggaaat cgttgatacc gatccgggcc
    15421 tgaaaggtga caccgcaggt actggcggca aaccggctac cctgtctact ggcgctgtgg
    15481 ttaaagttcc gctgttcgtg cagatcggcg aagtcatcaa agtggatacc cgctctggtg
    15541 aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc ctgcaggctg caccatcact
    15601 ttttcaggtc agagatgatg aaacgcctta tcgttcttgt tttgcttgcc agcgcgctgc
    15661 tcacgggctg taacaccgct cgcggtttcg gcgaagatat caaacatctc ggcaactcca
    15721 tctcccgcgc tgccagctaa tttttcttct cctccgaaaa atcatcagat ttccatcatt
    15781 tttggcgatg ttgtctatta ttaatttgct ataggcaaac ataaataaca ttacctaaaa
    15841 ggaagacgtt atggtgaaga agacaattgc agcgatcttt tctgttctgg tgctttcaac
    15901 agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa gacatttctg atggcggtaa
    15961 cgcgatttct ggcgcagcaa cgaaagcgca gcaataagca ataacggtac gacagctgtg
    16021 tcgtgccgtt tgttttttct gcgatagtta cataggtaat agttgaaatt cccctgccac
    16081 ctggcaaaat atccgttcaa ccatcagctt tgcaggacga cctgcaaacg cgtcttttca
    16141 ccggggacgg ccccaatttt ctggagcctg atatgtcctg gattatctta gttattgctg
    16201 gtctgctgga agtggtatgg gccgttggcc tgaaatatac tcacggcttt agtcgtttga
    16261 cgccaagtgt tattaccgtg acggcgatga ttgtcagtat ggcgctactt gcctgggcga
    16321 tgaaatcgtt accagtaggg acggcttatg ccgtgtggac gggtatcggc gcagtcggcg
    16381 cggccatcac cggcattgtg ctgctcggtg agtccgctaa cccgatgcgc ctggcgagtc
    16441 tggcgttaat cgtattgggg attattggtc tgaaactcag cactcattaa ctaccgggct
    16501 gctgtaccca aataaactta ctgacatcaa acccttcccg ggtcgcgact gccagcatct
    16561 cctgtttcac ttcgtcagaa atggttggcg tgcgggagag tatccacagg tagtcgcggt
    16621 ccgggccgca aaccagcgca tggcggtatt cccgatcgag tgcaataacg ttataaccgc
    16681 catagaaagg accaaagaat gacactttca gcgcagcgcg agttggtgcg ccggtaaagt
    16741 acgctttccc ttcactctgc tgccacattt ctctgtcagg gttatagcct ttattaatga
    16801 cattcaggcc gccgtcatca cgcaggctgt atgttgcggt gactttttcc agtccacgtt
    16861 caaagcggtg atcaaaacgg gcaatctcat accaggtacc gagataacgt ttagcgtcga
    16921 aattattcac tacggtcacg ccacgcggcg gcgtagggga actgcaggca acgaccagaa
    16981 atgcagctgt cgctgcggca acgagaggga gcaggcgcat aaatgtttcc ttactggttt
    17041 ttttctaagt gtagatgaca gcaaggaaaa gcggagaaaa ggtccgaaaa ttcggacccg
    17101 atgaaatttt actgtagagc gttgagaatc tgccaggcgg cggcgactct cgctggattg
    17161 gggtagtttt tgttagccag catcacgata cccagctctt tttctggaat aaacgcgaca
    17221 tagctaccaa atccgccggt cgcccccgtt ttatgtaccc atgatgcgcg tactgcagga
    17281 gttgggggcg taatcggttt tacggggcgt gctgccagtg caattttatt gtcactgccg
    17341 ttaatgatga tgtcaggatt taccggccag tccagcattt cccagcccag accctgatac
    17401 atatcgccgg tttgccagta gcgagattgt gccagttgta tcccttgttg aagtgttttc
    17461 tcattgatat caaggggttt taaattgctt tgcacccagc gggccatatc ttcaatggtc
    17521 gacttcacac cataagcttc agcatctaac gcccctggcg aaacatgtac tgccttacct
    17581 tcgcgatatc cccaggcgta gttcttttct tctgcggacg gtacattaat ccacgtatga
    17641 ttgagtttga gtggctggaa gacacgagtt ttcatcgcct gctcaaaact caaaccagac
    17701 ggcttcacag ccagtgcgcc gaacaaaccg atactggagt tggcatacag acgttgtgtt
    17761 cctggagccc atgcaggctg ccagttttga tagaagcgca gcaagtcgct tgaggatttc
    17821 acctcatccg gcacctgcaa tggcaggccg ccagcggtgt aggttgcgag atgtaatagt
    17881 gtgatcccat tccactgttt agcggtaagt tcaggccagt attttgttgt gggatcgctt
    17941 aacttgattt cccctcgagc aatagcgtcg ccaccaagca cgcccgtaaa tgttttgctg
    18001 accgaaccta actcaaacaa cgtttgctgt gtgacgggct gctttttggc gatgtccgca
    18061 tagccccagg taaagtaata aggtttaccc tgataaatta ccgccaccgc cataccgggg
    18121 atcttttgtt gctctataag cggggtaatt gtgcgatgca caatatcgtt gatttgttga
    18181 ggggcggcaa atgtggagca agaggcggta attaataagg tgcagagtgt cgttttgagc
    18241 ataaggtctg gtttccatac aaaacggccc gccataggcg ggccggattt acattggcga
    18301 tgcgttagat tgtaacgaca ccaatcagcg tgacaactgt caggatagca gccagaccgt
    18361 agaaaaccca tttacccgca ggtacgtgga ttttcagatc gtgcatcgcg tggtgcatac
    18421 ggtgtaaacc acaccacagc ggcagaacga tcatcaggaa caggaatacg cgaccaatga
    18481 agctctgcgc gaacgccaga acgcgctcgt agctcagtgc atcacccgga aacagcccta
    18541 gcggcagcag aataccaacc agcaggatca tcaccggcgc aatgatggcg ctccacatac
    18601 caccggcccc gaagaggccc cagaataccg gttcgtcaga acgctttgga tttggattaa
    18661 tcatctcagg ctccttacca gtacagggca acaaacagga ttacgatggt ggcaaccaca
    18721 gttaccgccc agagactttt gataattggc tctggtccca ttttttcgtc ttttacaatg
    18781 atattggccg cttttggtgc cagctcaaac caggttttgg tgtgcagcag ggctgccgcc
    18841 agagtgatca ggttaatgat catgataacc gggttttgta aaaagtcgac gaatcccgcc
    18901 caggcttccg ggccattttt cagggcaaac agcccgaaaa tcagttcaat gctgaaccac
    18961 acagccggaa ccgccgtgcc ttcgcgcagc atgtaaaagc gataaaacgg caattttttc
    19021 caccaggtgg acgtcattgg ccgtacatac ggtttacgtt tagtcgtcat gttgcactcc
    19081 ttagcgtggt ttcagggtcg cgataagaaa gtctttcgaa ctttctactt tgccctgctg
    19141 aatggccgca gccggatcga cgtgtttcgg gcagacttcg gagcagtagc ccacgaaagt
    19201 acagctccat acgccgttct ggctgttcaa ctgcgccata cgctccttct taccgtggtc
    19261 gcggctatct tcgttataac gatgcgccag cgtaatggca gccggaccga tgaactctgg
    19321 gttcaggcca aactgcgggc acgcggcgta gcacagacca cagttgatgc aaccggagaa
    19381 ctggtgatac ttcgccatct gcgccggggt ctggatgtta gtaccctgat ccgcggtgcg
    19441 ggagttgccg atgatgtacg gtttgatcgc ttccagactt tcgatgaagt gagtcatatc
    19501 gaccaccaga tcgcgttcaa tcgggaagtt agctaacgct tcaaccttca taccgtcggt
    19561 gtaatcacgc aggaaggttt tacatgccag ttttggcacg ttgttaacca tcatgccgca
    19621 ggagccacaa atcgccatac ggcaggacca gcggtagctc aggtccggtg ccaggttgtc
    19681 tttgatgtag cccagcgcat ccagtaatga ggtagttgcg tcataaggca cttcatagaa
    19741 tgcgctatgc ggtgcggtat cgacttccgg gttatagcgc accacctcaa ttttcaggtt
    19801 tttcatctca gccattcgcc ttctccttct tattggctgc ttccgcctta tcggctgcat
    19861 ccgcttcgcc accgtaaaca cgtttagctg gcggcagcgt agtaatcttc acgtcgctgt
    19921 actccaggcg agtcgtgcca tcagcatcgc ggaaggcgag ggtgtgtttg aggaagttga
    19981 cgtcgtcacg ctcggtgcaa ccttcgtcca gacgctggtg cgcgccgcgg gactctttac
    20041 gtgccattgc ggagtgcgcc atacattcag caacgttcag accgtggccc agctcaatgg
    20101 tgtagagcag gtcggtgttg aacacgctgg aagtgtcggt gatgcgcacg cgcttgaagc
    20161 gttcctgcag ctctgccagc ttgtcgatgg ttttctgcat cagttccggc gtacggtaga
    20221 taccgcagcc ttcttccatg gccatgccca tttcgtcgcg gatcttagcc cagttttcgc
    20281 cgccatcctg gttaaccaga tctttcagac gttgttcaac gccagctgcc tgcgcttcaa
    20341 ttgccgcttc gttgccatta ccggcagttg ctgcacgctc tgtcgcttgt tcaccggcca
    20401 gacgaccgaa gaccaccagt tccgccagcg agttggagcc cagacggttc gcaccgtgca
    20461 gaccaacaga ggaacattca cccacggcga acagaccttt aatgcgggtt tcacagttct
    20521 gatcggtttc gataccgccc atggtgtagt gtgcggtcgg acgtaccgga atcggttctt
    20581 taaccggatc gacgccaacg tacgctttcg ccagttcgca gatgaacggc agacgttcat
    20641 gcagtttttt ctcgccgagg tgacgcaggt cgaggtaaac cacatcgcca cgtggcgtgg
    20701 agatggtgtt gcctttacgc cattcgtgcc agaaggcctg agaaactttg tcgcgtggac
    20761 ccagttccat atatttgttt ttcggctcgc ccagcggagt ttccgggccc atgccgtaat
    20821 cttgcagata acggtagcca tttttgttga ccagaatacc accttcaccg cggcagcctt
    20881 cggtcatcag gataccggaa cctggtaggc cggttgggtg atactgaacg aattccatgt
    20941 cacgcagcgg aacgccgtgg cttagcgcca tacccatacc gtcaccggta acgatgccgc
    21001 cgttggtgtt gtaacgataa acgcgacccg caccgccggt agccataacg accgcgttag
    21061 cacggatctg caccagcgtg ccttccatca tgttcattgc taccaggccg cgaacatgac
    21121 catcatcaac cagaatatcc agcacgaaat gttcgtcaaa acgctggatc tgcgggaatt
    21181 gcagagaggt ctggaacagc gtgtgcagca tatggaagcc ggtcttatcg gcggcgaacc
    21241 aggtgcgctc gattttcatg ccgccgaagc gacgtacgtt gacgctacca tccgggcgac
    21301 ggctccatgg gcacccccac agttccagtt gggtcatttc ggttgggcag tggtggacga
    21361 aataatccac gacatcctgc tcacacaacc agtcgccacc cgctactgta tcgtgaaagt
    21421 gatattcgaa gctgtcatga tcctgcgcga cagcggcgga gcccccttct gcagcaacgg
    21481 tatggctacg catcgggtat acttttgaga ttagtgcgat ttttgcattt ggatttgcct
    21541 gcgcggcagc aattgcagca cgtaatcccg cgccaccggc gcctacaatg gcaagatcgg
    21601 cttgaaaggt ttgcacgaca ttcctccaga ttgtttttat cccacagccc cgtacttcag
    21661 ggtaagtgcc tgaaagttac ggtctgcgaa cgctattcca ctgctccttt ataggtacaa
    21721 cagtatagtc tgatggataa gtctgaaatt tgacgagatc gattttttta gtgcgcaaga
    21781 gggctaaatt atcactgaag atgattaatt taattactaa accatcagat cgtgcttttt
    21841 ttagtcactg cccaccgttg tttgattcct gcgtaaaatt tgtctcgccg tcgcgttgcg
    21901 agtagacttc gtgcccttgt caaaaactgg agatttaact atgagcgaaa cggcatcctg
    21961 gcagccgagc gcatccattc ctaacttatt aaaacgcgcg gcgattatgg cggagatccg
    22021 tcgtttcttt gccgatcgtg gagtgctgga ggtggagacg ccttgtatga gccaggcgac
    22081 ggtaaccgat attcatttgg tcccgtttga gacacgtttc gttggccccg ggcattcgca
    22141 ggggatgaat ctctggttaa tgaccagccc ggaataccat atgaagcgcc tgctggttgc
    22201 cggttgtggg ccggtattcc agctgtgccg cagcttccgt aatgaagaga tggggcgtta
    22261 tcacaaccct gagttcacta tgcttgagtg gtatcgaccg cactatgata tgtaccggtt
    22321 gatgaacgag gtggacgatc tcttgcaaca ggtgctggac tgtccggcag cagaaagcct
    22381 ttcttatcaa caagctttct tacgttatct ggaaattgac ccactctctg ccgacaaaac
    22441 gcaactgcgg gaagtggcag cgaaactgga tttgagcaac gttgccgata ctgaagaaga
    22501 ccgcgacacg cttctacaat tgctgtttac ctttggcgta gagccaaata ttggcaaaga
    22561 aaaaccgacc tttgtgtacc actttccagc cagccaggca tcactggcgc aaatcagtac
    22621 cgaagatcat cgggtcgctg aacgctttga ggtttattat aaaggtattg agctggcgaa
    22681 tggtttccat gaattgacgg atgcccgtga gcagcaacaa cgctttgaac aagataaccg
    22741 taagcgcgcg gcgcgcggtt tgccgcagca ccccattgac cagaatctga ttgaagcctt
    22801 gaaagtcggt atgcctgact gttccggcgt ggcattaggc gttgatcgtc tggtgatgtt
    22861 ggcgctgggc gcggagacac tggctgaagt catcgccttt agcgttgacc gggcataatt
    22921 ctgaaaaatt actgaattaa ccgcgaagaa tggcgtgata agcgccattc tgtagcatat
    22981 gttttcattt ttccgctaaa ggccacaatt taagactgga tatttgatat catccaggta
    23041 tcaattctgt attggttttt acgctgcccg ctcaatgttg ggcagtgcag ttcagtgggc
    23101 gcgttttata cacgcgctga aatgaaggat ggtttcatgc ctcacacgat aaaaaagatg
    23161 agtctgatag gactcatatt gatgatcttt acttctgtat ttggatttgc caatagccca
    23221 tcggcttatt acttaatggg ttatagtgcg attccctttt atatattttc tgcattgtta
    23281 ttctttattc cattcgcctt aatgatggct gaaatgggag ctgcttatcg caaagaagaa
    23341 ggcggtatct attcctggat gaataatagt gtcggaccac gttttgcctt cattggtacg
    23401 tttatgtggt tttcctctta tatcatctgg atggtgagta cctccgcgaa agtttgggta
    23461 ccgttctcaa cattcctcta tggtagcgac atgacccagc actggcgtat tgccggactg
    23521 gagcctacgc aggtggttgg tctgctggca gtggcatgga tgattctggt caccgtcgtt
    23581 gcttctaagg ggattaataa aattgcccgc attactgcgg tgggcggtat tgcagtaatg
    23641 tgtctgaatt tagtattgct gttagtaagc attactattt tgttattaaa tggtgggcat
    23701 ttcgcgcagg atattaattt ccttgcatca ccgaaccc
//
LOCUS       DAZGJT010000074        23624 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZGJT010000074 DAZGJT010000000
VERSION     DAZGJT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.074"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..154
                     /locus_tag="ACHKFK_003188"
     CDS             <1..154
                     /locus_tag="ACHKFK_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFO9053462.1"
                     /translation="IHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALDKIKLFG
                     SDGKAE"
     gene            159..1106
                     /gene="ydjH"
                     /locus_tag="ACHKFK_003189"
     CDS             159..1106
                     /gene="ydjH"
                     /locus_tag="ACHKFK_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9053463.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGRALTEIFTQA
                     KARQMIICADMIKPRLNETLNDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSIGATTGVKNRKLVEQLLEEYEG"
     gene            1116..2096
                     /gene="ydjG"
                     /locus_tag="ACHKFK_003190"
     CDS             1116..2096
                     /gene="ydjG"
                     /locus_tag="ACHKFK_003190"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFO9053464.1"
                     /translation="MKKIPLGTADITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPKSIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNALKAE
                     GKIRAIGAANVDADHIREYLQHGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            2233..2991
                     /gene="ydjF"
                     /locus_tag="ACHKFK_003191"
     CDS             2233..2991
                     /gene="ydjF"
                     /locus_tag="ACHKFK_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9053465.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            3109..4467
                     /gene="ydjE"
                     /locus_tag="ACHKFK_003192"
     CDS             3109..4467
                     /gene="ydjE"
                     /locus_tag="ACHKFK_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053466.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGRIAGAESQLR
                     EVEQQIEREKSIRLPPLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(4560..5201)
                     /gene="pncA"
                     /locus_tag="ACHKFK_003193"
     CDS             complement(4560..5201)
                     /gene="pncA"
                     /locus_tag="ACHKFK_003193"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001587135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO9053467.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLKQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRAHVINELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQE"
     gene            complement(5212..6228)
                     /gene="ansA"
                     /locus_tag="ACHKFK_003194"
     CDS             complement(5212..6228)
                     /gene="ansA"
                     /locus_tag="ACHKFK_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO9053468.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(6395..8251)
                     /gene="sppA"
                     /locus_tag="ACHKFK_003195"
     CDS             complement(6395..8251)
                     /gene="sppA"
                     /locus_tag="ACHKFK_003195"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO9053469.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSSAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKTLTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITKALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            8412..8963
                     /gene="ydjA"
                     /locus_tag="ACHKFK_003196"
     CDS             8412..8963
                     /gene="ydjA"
                     /locus_tag="ACHKFK_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO9053470.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDNKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            9080..10123
                     /gene="selD"
                     /locus_tag="ACHKFK_003197"
     CDS             9080..10123
                     /gene="selD"
                     /locus_tag="ACHKFK_003197"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO9053471.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            10128..12083
                     /gene="topB"
                     /locus_tag="ACHKFK_003198"
     CDS             10128..12083
                     /gene="topB"
                     /locus_tag="ACHKFK_003198"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO9053472.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTSVRQFRGIMAPGGRE
                     GKKKDSPRKRAPKKSPPSEEAGNGVIT"
     gene            complement(12160..13503)
                     /gene="gdhA"
                     /locus_tag="ACHKFK_003199"
     CDS             complement(12160..13503)
                     /gene="gdhA"
                     /locus_tag="ACHKFK_003199"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO9053473.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVTDYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLSQGVI"
     gene            13739..14011
                     /gene="ynjH"
                     /locus_tag="ACHKFK_003200"
     CDS             13739..14011
                     /gene="ynjH"
                     /locus_tag="ACHKFK_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO9053474.1"
                     /translation="MSRALIAVVLAFPLITLANPHYHPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            complement(13977..14384)
                     /gene="nudG"
                     /locus_tag="ACHKFK_003201"
     CDS             complement(13977..14384)
                     /gene="nudG"
                     /locus_tag="ACHKFK_003201"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO9053475.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAHSDQAGLWEFAGGKVEPDES
                     QRQALVRELNEELGIEATVGDYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALRYPLAPADIPLLEAFMASRAARPAD"
     gene            14471..15091
                     /gene="ynjF"
                     /locus_tag="ACHKFK_003202"
     CDS             14471..15091
                     /gene="ynjF"
                     /locus_tag="ACHKFK_003202"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO9053476.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVAILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALVGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGSET
                     ILLFVLSCLFPACFPWFAWVFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(15100..16407)
                     /gene="ynjE"
                     /locus_tag="ACHKFK_003203"
     CDS             complement(15100..16407)
                     /gene="ynjE"
                     /locus_tag="ACHKFK_003203"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO9053477.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNEWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKFYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAAARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPDQQVSFYCGTGWRAS
                     ETFMYARAMGWNNVSVYDGGWYEWSSDPKNPIATGERGPDSSK"
     gene            complement(16474..17127)
                     /gene="ynjD"
                     /locus_tag="ACHKFK_003204"
     CDS             complement(16474..17127)
                     /gene="ynjD"
                     /locus_tag="ACHKFK_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9053478.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFSVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRHWVFSEIRAMAIPVVQVTHDLQDVPPDSPILDMAQWSENYNKLR"
     gene            complement(17127..18662)
                     /gene="ynjC"
                     /locus_tag="ACHKFK_003205"
     CDS             complement(17127..18662)
                     /gene="ynjC"
                     /locus_tag="ACHKFK_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO9053479.1"
                     /translation="MATPLRYALIFLLWAMMAVIYAPLIPAALTLISLALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQLIVLDSLGYSRWQCLNWLLLPSVASALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLILLLAAYVLLGYLLWRGWRRTIPRVDGVRKP
                     ATPLLPGNTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVAAFIALLLLL
                     LWQEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPALIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLSLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            complement(18635..19801)
                     /gene="ynjB"
                     /locus_tag="ACHKFK_003206"
     CDS             complement(18635..19801)
                     /gene="ynjB"
                     /locus_tag="ACHKFK_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9053480.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPTALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKANTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFITIPANANASAAAKVVANFLLSPDAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            complement(19811..20359)
                     /gene="ynjA"
                     /locus_tag="ACHKFK_003207"
     CDS             complement(19811..20359)
                     /gene="ynjA"
                     /locus_tag="ACHKFK_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO9053481.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRARLATHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(20359..21066)
                     /gene="ydjZ"
                     /locus_tag="ACHKFK_003208"
     CDS             complement(20359..21066)
                     /gene="ydjZ"
                     /locus_tag="ACHKFK_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9053482.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAVLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            complement(21080..21757)
                     /gene="ydjY"
                     /locus_tag="ACHKFK_003209"
     CDS             complement(21080..21757)
                     /gene="ydjY"
                     /locus_tag="ACHKFK_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO9053483.1"
                     /translation="MLQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMGYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKASSFDEVIVDSNGKKLNMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            complement(21762..22472)
                     /gene="ydjX"
                     /locus_tag="ACHKFK_003210"
     CDS             complement(21762..22472)
                     /gene="ydjX"
                     /locus_tag="ACHKFK_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO9053484.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLSHPKNEG"
     gene            complement(22639..23445)
                     /gene="xthA"
                     /locus_tag="ACHKFK_003211"
     CDS             complement(22639..23445)
                     /gene="xthA"
                     /locus_tag="ACHKFK_003211"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO9053485.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSPLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRENPVLIMGDMNISPGDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMERLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
BASE COUNT         5859 a         5894 c         6186 g         5685 t
ORIGIN      
        1 cattcatacg gagctctgtc aggccgcaat ggtagcagtg aaagaaaacc aggatcagcc
       61 tttcctgcat ttagaacgtg aagtgcgtaa agctgtaaaa gaacgggcat tggataaaat
      121 taaactgttc ggttctgatg gcaaagcgga ataatgatat ggataatctc gacgttattt
      181 gtataggtgc cgctattgtt gatattccat tgcaaccggt cagtaaaaat atctttgatg
      241 tggattctta tcctcttgaa agaatcgcaa tgaccaccgg gggtgatgca ataaatgaag
      301 caacaattat ttctcgtctg ggccatcgca cagcattaat gagtcgtatt ggtaaagatg
      361 ccgcagggca atttattctc gaccattgcc gcaaagagaa tattgatatt caaagcctga
      421 agcaggacgt cagcatagat acctctatta acgttggact ggtgacggag gatggcgagc
      481 ggacctttgt cacaaaccgt aacggcagtc tgtggaaatt gaatattgac gatgttgatt
      541 ttgcgcggtt ttctcaggcg aaattattat cactggccag tattttcaac agtcctctat
      601 tggatggtag agcgctaaca gaaattttta cacaagctaa agcccggcag atgattatct
      661 gtgccgatat gatcaaaccg cggttgaatg aaacgctgaa tgatatttgc gaagcattga
      721 gttatgtcga ttatctgttt cctaattttg ccgaggcaaa attactcacc gggaaagaga
      781 cactggatga aattgctgac tgctttcttg cgtgcggcgt aaaaacggtg gtgattaaaa
      841 cgggtaaaga cggctgcttt atcaagcgtg gtgacatgac gatgaaggtg ccggcggtcg
      901 caggaataac cgctatcgac accattggtg cgggcgataa ctttgcttca ggttttattg
      961 cagcactgtt agaaggcaaa aatctgcgag aatgcgcacg ctttgccaat gcaacggcgg
     1021 ccatctcggt tctaagcatt ggcgccacca ccggtgtaaa aaacagaaag ctggtcgaac
     1081 aattgctgga agaatacgaa ggataatgaa ggcaaatgaa aaagatacct ttaggcacag
     1141 cggatataac gctttcgcga atggggttgg ggacatgggc tattggcggc ggtcctgcat
     1201 ggaatggcga tctcgatcga caaatatgca ttgatacgat tcttgaagcc catcgttgcg
     1261 gcattaacct gattgatact gcgccaggat ataactttgg caatagtgaa gttatcgttg
     1321 gtcaggcgtt aaaaaaactg ccccgtgaac aggttgtagt agaaaccaaa tgcggcattg
     1381 tctgggaacg aaaagggagt ttattcaaca aagttggcga tcggcagttg tataaaaacc
     1441 tttctccgaa atctatccgc gaagaggtag aagccagctt gcaacgtctg ggtattgatt
     1501 acatcgatat ctacatgacg cactggcagt cggtgccgcc attttttacg ccgatagctg
     1561 aaactgtcgc agtgcttaat gcgttaaagg ccgaagggaa aattcgcgct ataggcgctg
     1621 ctaacgtcga tgctgaccat atccgcgagt atctgcaaca tggtgaactg gatattattc
     1681 aggcgaaata cagtatcctc gaccgggcaa tggaaaacga actgctgccg ctatgtcgtg
     1741 ataatggcat tgtggtacag gtctactctc cgctagagca gggattgttg accgggacca
     1801 tcacgcgtga ttacgttccg ggcggcgctc gggcaaataa agtctggttc cagcgtgaaa
     1861 acatgctgaa agtgattgat atgcttgaac agtggcagcc actctgtgct cgttatcagt
     1921 gcacaattcc cactctggca ctggcgtgga tattaaaaca gagtgattta atctccattc
     1981 ttagtggggc tactgcgccg gaacaggtac gtgaaaatgt cgcggcactg aatatcaact
     2041 tatcggatgc agacgcaaca ttgatgaggg aaatggcaga ggccctggag cgttaaatac
     2101 tcaacacgat tcacctctgt ttctgtgaaa actgtgatga aaatctgtga ttcaaacagg
     2161 ttattttgaa agtaaacatc ggttatgcga taattgcgct aatgtgatgt aaaaagatac
     2221 aggggtgtaa tcgtggcggc aaaagacagg attcaggcaa ttaagcaaat ggttgccaac
     2281 gataaaaagg tgacagtctc aaatttgagt gggatttttc aggtaaccga agaaaccatt
     2341 cgccgcgatc ttgagaagtt ggaagatgaa ggctttttga ccagaaccta tggtggtgct
     2401 gttttaaata cagcgatgtt gacggagaat atccattttt ataagcgcgc ttcatcgttt
     2461 tatgaagaga agcagcttat tgcacgcaag gcactaccct ttatcgacaa taaaaccacc
     2521 atggcagccg attccagtag taccgtaatg gaattgctca aattgttaca ggaccgtagt
     2581 gacctgacgt tgctaaccaa ctcggcggaa gcgattcatg ttctggctca gtcagaaatt
     2641 aaagtcgttt caacgggtgg ggaactaaac aaaaatacac tttcactgca aggaagaatt
     2701 actaaagaga tcatcagccg ctaccatgtc gatatcatgg taatgagttg taaaggtctt
     2761 gatattaaca gcggcgcgct ggactctaat gaagcggaag ctgaaatcaa aaagacaatg
     2821 atccgtcagg cgacagaagt tgcgttattg gttgatcact ctaagtttga tcgcaaagct
     2881 tttgtccagt tagctgactt tagtcatatt aattacataa taactgataa atcaccgggt
     2941 gcagagtgga tagcattttg caaagacaat aatatccagc tggtgtggta atttaagcac
     3001 acattgtcaa tattatttgt tcctgcgcct ggaaataaaa tgatgataaa gatgcggcaa
     3061 gtgaatttgc cgcaattgct acctttttta gcaaatccga ggcacccaat ggaacaatat
     3121 gatcaaattg gcgcaagact ggaccgcttg cctttggccc ggtttcatta tcgtatattt
     3181 ggtataataa gctttagtct gttattaacg gggtttttga gttactccgg taatgtcgtc
     3241 ttagcaaagc tggtaagcaa tggatggtca aataatttcc tcaatgccgc ctttacctcg
     3301 gcattaatgt ttggttattt catcggctct cttaccggtg ggtttattgg tgactacttt
     3361 gggcggcgca gggcgtttcg cataaatctt ctcatcgtcg gtattgcggc aacaggggcc
     3421 gcttttgtcc ctgatatgta ctggctcatc ttctttcgct tcctgatggg aacaggaatg
     3481 ggggcgctga ttatggttgg ctatgcctca tttacggagt ttatccccgc gacggtgcgt
     3541 ggaaaatggt ccgcgcggct ctcatttgtt ggaaactggt cgcccatgct gtctgcggcg
     3601 ataggcgtgg tggttatcgc tttttttagt tggcggataa tgtttctgct gggcggtatt
     3661 ggcatactgt tagcctggtt tctctcaggt aaatacttta ttgagtcgcc acgatggttg
     3721 gcagggaaag ggcgaatcgc aggtgcagaa agccaacttc gtgaagtaga gcagcaaatt
     3781 gaaagagaga agagtattcg tttacccccg cttactttga accagagcaa cagcaaggtt
     3841 aaggtaatca agggtacctt ctggctcctg tttaaagggg aaatgttacg acgtacatta
     3901 gtcgcgatta ctgttttaat tgcaatgaac atttcgcttt ataccatcac cgtatggata
     3961 ccgaccatat ttgttaactc cggcattgat gtcgataaat caatattaat gaccgctgtt
     4021 attatgattg gcgctccggt agggatattt attgcggcat taattattga tcatttccct
     4081 cgtcgattat ttggttccgc cttacttatt attattgccg tgttaggcta tatctattca
     4141 attcagacaa cagagtgggc gattttaatc tatggcctgg tgatgatctt ctttttatac
     4201 atgtatgttt gcttcgcgtc ggcggtttat atcccggaac tttggccaac gcatttacgc
     4261 ctgcgtggtt cgggtttcgt taatgccgta ggacggatcg tcgcagtctt cacgccctat
     4321 ggcgttgcgg cattattaac acattatggg tcgatcacgg tgtttatggt gcttggtgtc
     4381 atgttagtgc tctgtgcgct ggttctctcc atttttggca tcgaaacgcg gaaggtgtcg
     4441 ctggaagaga tttctgaggt gaattaaatc tttcgttgat ggcgtcaatt accagatgta
     4501 tcactgatgt gttttatctg gcctacaatg ccagtacaga attgtaggcc agcgtaaaat
     4561 tactcctgcg tttcttccca gtctgccagc gtatatagcg ttgccccagc tgctgacatt
     4621 tccataaacg cgtgcgcact gtcctgtggc tggatattta cgccacgaca accatcggta
     4681 atcacgttca ccttatagcc taactgtaac gcgtccagca cggtaaactt aacgcaatag
     4741 tcagtagcca gacccataac gatcaattca ttgattacat gagcgcgcaa ccagtcatcg
     4801 agcgcagttt tctgccgacg gccgttatca aaaaaggcac tgtaactgtc aactaaagga
     4861 ttttcgcctt tatggaacac cgctgcgatc gctttttgct tcagtaacgg atgtaattgt
     4921 gcgccttcac tgttctgcac acagtgatcg ggccagaagg tttgtggcaa accgtcgagt
     4981 tggcccggcg tataaggctc tacaccatgc tgactggcaa aactgccgtg attcgccgga
     5041 tgccagtcct gactggcgat aaccgcttca ccgcgcgact ggcaccagtc aatcaggcgg
     5101 ttagcgacat ccaccgtact gtcaccttcc ggcacggcga gcgcgccacc agcacagaaa
     5161 tcattttgta aatcgaccag taacagggcg cgagggggca ttgcagtctc cttaatcatc
     5221 cggcgtcagt tcgccacgca ggttttggct catggccttg cgaatggttt cagtatcaag
     5281 ttcctggcta agcaggtaat gcagtttggt tagcgtggct tctacagtca tatctgcacc
     5341 gccaataacg ccggcatggg cgagggcgtt accagtggca taaccaccca tgttcacttt
     5401 accggacata cattgtgtca ggttgaccac cacaataccg cgatcgctgg cttcttgtaa
     5461 ttcctgcagg aaggctttgt tttgtggcgc attacccacg ccatatgagc gcagaatcaa
     5521 tgctttcaca ggttggcgca gaaaattgcg cacgacgtca gcagaaatcc ccggataaat
     5581 cgtcactacg ccaattggtt gcggcgtgat tggatgaacg atcaattccc cttcaccgtg
     5641 cggggcgggt ggcgtattca aacgacgaat atggatacct gcttccagta acggaggaag
     5701 gtttggagag gcaaacgcat caaaaccatc ggcatgggct ttggtggttc ggttgccacg
     5761 atacagtcga ttattaaaaa agagagttac ttcgttgata ggataattcg ccgcaacgta
     5821 caacgcattc agtagattaa tttgtccgtc agagcgtaac tcagccagcg ggatttgtga
     5881 ccctgtcaca atgaccggtt taccgagatt ctcgagcatg aacgacagtg cagaggcggt
     5941 atacgccatc gtgtctgtgc cgtgcagaat gacaaaacca tcatagtcgt catagtgcgc
     6001 tttaatatct tcagcaatat gctgccagtc ttctggcgtc atatcagaag aatccatcaa
     6061 tggcgtatat tcatgaatgg tgaaatctgg catctccggg cgatggaatt ccggcatcag
     6121 cgccagttgg cgttgtagat gacctgacac cggaatataa ccctgctcgg aacgctgcat
     6181 cccgatggtc ccgcccgtgt aggcgacgta aattgatttc ttttgcatga tattgatatg
     6241 aatgatatcg aaagagcaaa agtatatacg tgaggcaggg ggagcgcacc tgatactatg
     6301 aggataagga aatatgtggc gagacagcgg gaagaggatt cgtcgacgta aaaagtaatg
     6361 tggccgacga tctgtcggcc acttagtaca agacttaacg cacgttggcg caggtcaggc
     6421 aaaacgcata acggttttgt gggtcgttaa acgcggccag cttgtcgctt tcacttttaa
     6481 cggtagaggc taccgaggcc agcggtgcag gtaacatggc ctggaacgca tctggcaaca
     6541 ttgcccggac agaaccagac atgttgtcca tcactttgtc gaagaaggtc ggttcatcaa
     6601 cgtagtattc cagatgccac tgtttcactt ttgccagctc tgctgctttg gcgaccgcat
     6661 catcgaaatc cccgagacta tcgaccagcc cgttagcttt tgcatcctga ccggtccaga
     6721 cgtgaccctg ggcgatttta tcaatctgct ccggcgttga atgacgcgca tcagcaacca
     6781 gcgtgataaa gcgtttatag ccattctcaa tgcttaactg catcatctgc tgcgcttccg
     6841 gcggcagtgc cttggtgata gaaacatctg ccagcggtga agttgagaca ccatcagtat
     6901 gaacaccaat cgaatccaga ctattttcta cggtggtgat cacgccgaag ataccgatag
     6961 aaccggtcag ggtgctgggg ttagccacaa tgtaattagc tggtgtggaa atccagtaac
     7021 caccagatgc cgccatgccg cccatcgata caaccacagg cttacccgct gcccgggctg
     7081 ctgccagttc agcgcgaatc acttcagacg cggtaacgct gccgccggga ctattaacac
     7141 gcagaacaat cgctttcact ttcgggtcaa ggcgagcgtc gcggatttgt gccgcagtgg
     7201 tatcaccgcc aacattcccc tgagtttcct cgccatccat aattgcgcca ttagcgaaga
     7261 cgacaccgat actgtcaccg gtatctgccg gcgttttcaa tgcgtaatcg taataactga
     7321 tggcgcgata atttttatca gttttactcc agccgaactc tttggtcaga gttttttcga
     7381 tttcggcact cgatgccagt gcatcgacca gcttgttttc cagcgcatat ttcgcggtat
     7441 cgccaccggt tttggttaaa ccctcaagca acccttgcgc gccagggaat acctgctgag
     7501 cagggatctg gcggttagca gcaacagtat tcagatagtt ttgccacagc tcaccaatcc
     7561 agcggctgtc agcttcgcgg gctgccgacg acatatcatc acgaataaac ggttcaacgg
     7621 cagatttata cgtacccacg cggaacacat gggtggaaac tttcagctta tccagtaacg
     7681 atttgtagta cagaccgttg gtggcaaagc cgtgcagatc aaccacgcct tgcggagaca
     7741 gccaaatttt attggcgaaa ctggcgagat aatattgccc ctggctgtag ttctcgccaa
     7801 cggcataaac cggtttcccg ctgtcacgaa actctttcag agctttgccg atgtactgca
     7861 tagacggctg gtcgccgcct gcgaagtttt tcagatccat cacaataccg gtgatattgc
     7921 ggtcgtcctt cgcctggcga atggtgttga cgatatcaaa cagtgagttt tcctgcagac
     7981 gatcggaact ggcaccaagc agctggcggc ttaatttact aaaccgctga gaactgtcgg
     8041 gtttatcgac gatcacacca gaaatgtcca gcagcagtgc gccacgactg gccgtttctt
     8101 tcgaatcacc accactgacc tgcatccaga tccccacacc aaccagcacg agaaaaataa
     8161 agaacaggtt aagtaccatt tcacggacga aattcagcag acgccacgtc catttaaaaa
     8221 atccggcaat aaatcgccaa agggttcgca tgtattctcc caacttaagg tcacacctgt
     8281 ttcaggcgca atagatgtgg ttatcgtaat gacccaaccg gcacttgtca gcaggaattg
     8341 cggactgggc tgtaacaaaa tccactctcg tgttaatttt gtgaataaat atcatgacag
     8401 gagttaatca aatggatgca ctcgaactat tgatcaatcg ccgtagcgcc tcccgcctgg
     8461 ctgaacccgc gccaacgggt gaacaactgc aaaacatcct gcgtgcgggt atgcgtgcgc
     8521 cggaccataa gtccatgcaa ccgtggcatt tctttgtgat tgaaggggaa gggcgcgagc
     8581 gtttcagcgc tgtactggag cagggggcga ttgctgccgg tagtgataac aaagcaatcg
     8641 acaaagcgcg taatgcgccg ttccgcgcac cgctcatcat aacggtggtg gcgaaatgcg
     8701 aagaaaatca taaagtcccg cgctgggaac aggaaatgtc tgcgggatgc gcggtcatgg
     8761 cgatgcaaat ggcagcagtt gcccaggggt ttggcggcat ctggcgcagt ggtgcattaa
     8821 ctgaaagtcc ggtagtgcgt gaagcattcg gttgccgtga gcaggataaa attgtcggtt
     8881 ttctctacct cggtacgccg cagttgaaag catctacgtc gataaacgtc ccggacccga
     8941 cgccgtttgt gacttatttc tgataattgt cgcgaaactg tctggattgt gagcaaaggc
     9001 gcaggaattc agacactctc acttatcact tcatggaatg agggttacca tacctggatt
     9061 gcattgacag gagatgtcca tgagcgagaa ctcgattcgt ttgacccaat acagccacgg
     9121 cgctggttgc ggctgtaaaa tttccccaaa agtgttggaa accatcctgc acagtgagca
     9181 ggcgaagttt gttgatccga atttgcttgt gggtaatgaa acccgcgacg atgcggcggt
     9241 atacgatctg ggcaatggca ccagcgttat cagtaccacc gacttcttta tgccgatcgt
     9301 tgataatcct ttcgattttg gccgcattgc ggcgactaac gccatcagcg atatctttgc
     9361 tatgggcggc aaaccgatta tggcgattgc gatcctcggc tggccgatta acaaactttc
     9421 cccggaaatt gcccgcgaag tgaccgaagg tggacgctat gcatgccgtc aggcgggtat
     9481 tgcgctggct ggcggtcact ccatcgatgc gccggagccg atttttggtc tggcggtaac
     9541 ggggatcgta ccgaccgagc gggtgaagaa aaacagtacc gcacaagccg gatgcaaact
     9601 gttcctgacg aaaccgctgg ggattggcgt tcttaccacg gctgagaaaa aatcactgct
     9661 gaaaccagaa catcagggac tggcgacgga agtgatgtgc cggatgaaca tcgcaggcgc
     9721 gtcctttgcc aacatcgaag gcgtaaaagc gatgaccgac gttacgggtt ttggtttact
     9781 ggggcattta agcgaaatgt gtcagggcgc aggtgtgcag gcgcgcgtcg actatgacgc
     9841 gatcccgaaa ttgccggggg ttgaagagta cattaagttg ggcgcagtgc ctggcggcac
     9901 cgaacgtaac tttgccagct acggtcatct gatgggtgaa atgccgcgtg aagtgcgcga
     9961 tctgctgtgc gatccgcaaa cttctggcgg tttgctgctg gcggtcatgc cggaagcaga
    10021 aaatgaggtc aaagctacag ccgccgagtt tggcattgaa ctgacggcga ttggcgaact
    10081 ggtgccagcg cgcggcggtc gtgccatggt tgagatccgt taattcaatg cggttgttta
    10141 ttgccgaaaa accgagtctg gcgcgcgcca ttgctgatgt cctgcctaaa ccgcaccgga
    10201 aaggcgatgg ctttatcgag tgcggtaatg gtcaggtggt gacctggtgt atcggtcacc
    10261 tgcttgagca ggcgcagcca gacgcctacg acagccgcta tgcgcgctgg aatcttgcgg
    10321 atttgccaat tgtcccggaa aagtggcaat tacagccccg accctccgtg actaaacaac
    10381 ttaatgtcat caaacgcttc ctgcatgaag ccagcgaaat cgttcacgcc ggagacccgg
    10441 atcgcgaagg gcaactgctg gtggatgaag tgctggacta cctgcaactg gcaccggaaa
    10501 agcgccagca ggtacagcgt tgtttgataa acgacctgaa cccgcaggcg gttgagcggg
    10561 cgatcgatcg tcttcgctcc aatagcgagt ttgtaccgct gtgcgtttct gcactggcgc
    10621 gggcgcgggc tgactggctg tacggcatta atatgacccg tgcgtatacc attctcggcc
    10681 gcaatgccgg ttatcagggc gtgctttccg tgggacgcgt gcagacgccc gtactcggac
    10741 tggtggtgcg ccgcgacgaa gagattgaaa acttcgtggc gaaagacttc tttgaagtca
    10801 aagcacatat cgtgacacca gcagatgagc ggtttaccgc tatctggcaa ccgagcgaag
    10861 cgtgtgaacc gtaccaggat gaagaagggc gcttgttaca tcgtccactg gcggagcatg
    10921 tggttaaccg cattagtggt caaccagcta ttgtcaccag ctataacgat aaacgggaat
    10981 cagaatccgc gccgctgcct ttttcgcttt cggcgttgca gattgaagcg gctaaacgtt
    11041 ttggtctgag cgcgcagaac gtgcttgata tctgccagaa gctgtacgaa acgcacaagc
    11101 taatcactta tccgcgttcc gattgccgct atttgccaga agaacatttt gccggacgcc
    11161 acgcggtgat gaatgctatc agcgttcatg caccggatct gttgccgcag ccagtggtag
    11221 atccagatat acgcaaccgc tgttgggatg acaaaaaggt cgatgcgcac cacgccatca
    11281 ttccgaccgc acggagttct gcgatcaacc tgacggagaa cgaagcgaag gtctataacc
    11341 tgattgcccg tcagtatttg atgcagttct gcccggatgc ggtgttccgc aagtgtgtta
    11401 tcgaactgga cattgccaaa ggcaaatttg tcgctaaagc ccgttttctt gctgaagcag
    11461 gctggcgcac gctgttaggc agcaaagagc gcgatgaaga aaacgacggc acgccgctgc
    11521 ctgtggtggc gaaaggcgat gaattgttgt gcgaaaaagg tgaagtggta gagcggcaaa
    11581 cccagccgcc acgccatttt accgatgcaa cactgctttc ggcgatgacc gggatcgcac
    11641 gctttgtgca ggacaaagat ctgaaaaaga tccttcgtgc gaccgatggt ctggggacag
    11701 aagcaacgcg tgccgggatt attgaactgt tgttcaagcg gggtttcctg accaaaaaag
    11761 ggcgctatat ccactccacc gacgccggaa aagcgctatt ccattcgctg ccagaaatgg
    11821 cgacgcgacc ggacatgacc gcgcactggg aatcggtgct gacgcaaatc agcgaaaagc
    11881 agtgtcgcta tcaggacttt atgcagccgt tggtggggac attgtaccag ttgattgatc
    11941 aggcaaagcg cacgtcggta aggcagtttc ggggaataat ggctcccggc ggtagagaag
    12001 ggaagaaaaa ggattcgcca cgcaagagag cgccgaaaaa aagcccgcca tcagaagagg
    12061 cgggcaatgg agtgataaca taaaggttct ttcaaccatc tgatactgaa gatttttatg
    12121 gccggataag gcgtttgcgc tgtatccggc acaattgtct taaatcacgc cctggcttaa
    12181 catcgcatcc gcaaccttca caaaaccggc aatgttcgcg ccctgcacgt agttggtttg
    12241 ctcaccttca ccaccatgct caacacaggc atggtggata tccagcatga tgtgatgcaa
    12301 acgtgcgtca actttctcgg ctttccagcc caggcgcgca gcgttttgcg ccatttccag
    12361 gcccgatgtt gctacaccgc cagcattagc cgctttaccc ggtgcaaaca gtacgcctgc
    12421 ctgctggaac agttcagtcg cttcgatggt ggtcggcata tttgcccctt cggcgacggc
    12481 tttaacgcca ttggcgataa gctgatgcgc ggcgtcaaca tccagttcgt tctgggtggc
    12541 gcaaggcagg gcgatatcaa ccggtacaga ccacggctgt tggccttcga gatagaccag
    12601 accaaattct ttggcgtaat ctgtcactcg accatcgcgg ctggctttga tttcgataag
    12661 acgcgccagt ttttctttcg tgaatccgct ttcatcaact acagtgccgc tggagtctga
    12721 cgcagtgatc actcgagcac caaattccat cgctttttcg atagcgtact gggcgacgtt
    12781 gccggagcca gaaacggaaa cgcgcattcc ttcaaaaccc ataccgtggc gttttagcat
    12841 tgcttctgtg aaataaacca gaccgtagcc ggtagcttcc gggcgaataa gactgccgcc
    12901 aaatgaaagg cccttaccgg tgaagacgca ggcggtattg ttggagagct ttttcatcat
    12961 ccccgccata aagccgactt cacgaccacc aacgccgata tcacctgccg gaacgtcagt
    13021 atccgcgccc aggtggcgat acagttcagt catcagcgcc tggcagaaac gcataacttc
    13081 gccttcgctt ttccctttcg gatcaaagtc gctgccgcct ttaccaccgc ccatcggcag
    13141 ggttgtcagg gcatttttaa aggtttgctc gaagccgagg aatttgagaa tggaaaggtt
    13201 tactgaaggg tggaagcgca taccgccttt gtacgggccg atggcagagc tgaactgcac
    13261 acgccatgca cggttgacct gtacctggtt gcgatcgtca acccatacca cgcgaaactg
    13321 gatcacgcgc tccggttcaa ccagacgctc cagtaatgac atctgacgat atttgggatt
    13381 ttgttcaaga aaaggccaga gtgtggtcat tacttcacga acggcttgcg cgaactcggt
    13441 ttgattcggg tcgcgctttt ggacatggtt gaggaatgac tccagagaat atgtctgatc
    13501 catagatata aaacccttat atattaatac gattatgctt atgttgttta tgtcatgtgc
    13561 ttttgcagtt ttcgactata ccatcaagaa tgtgacggta agcaagaaaa aaatgaccca
    13621 ggaaagcaaa ttaataacga gagtaatctc ataaccaaat gcgataactc agacgctaaa
    13681 gttttgccgc ccggctaccg ttatctgtag aagagacaat aacgttaagg agttccctgt
    13741 gagtcgagca ttgatcgccg tagtattagc atttccatta attacactgg ctaacccgca
    13801 ttatcatccg gacgtggagg taaacgtacc gccagaagtg tttagttcag gcgggcagtc
    13861 agcgcagccc tgtactcagt gctgcgttta tcaggatcaa aattattctg aaggggcagt
    13921 aattaaagcc gaaggaatac tgctgcaatg ccaacgggat gacaaaaccc taagcactaa
    13981 tccgctggtc tggcggcgcg tgaagccata aacgcctcta ataatggaat gtcagcaggg
    14041 gccagcggat atcgcagcgc ctcttcaggt gagcaccaga ccagcgcctg atgttcatgt
    14101 gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat gaagatggat aatccgcccc
    14161 gaaacttctc gctgatggct ggcaacatag tcaccaacag ttgcttcgat gcccagttct
    14221 tcgtttaact cacgcaccag cgcctgccgc tggctttcat ccggctcgac tttaccaccg
    14281 gcaaactccc ataatcccgc ctgatcgcta tgggcagggc gttgcgcgag taaaattttg
    14341 ccatcacgtt cgatgatggc ggcaacaact tcaatcattt tcataaatta cacacgggca
    14401 gataagcgaa aaatgccata atatccgcac tgttgctcaa tcagaaaggc tttatattta
    14461 aggagtgcct gtgctagacc gccatcttca tccccggatt aaaccgttgt tgcatcagtg
    14521 cgtgcgggtt cttgataaac cgggcattac gccagatggt ttaacattag tgggatttgc
    14581 catcggcgtg ctggcactgc cttttctggc gctgggctgg tatctggcgg cactagtcgc
    14641 cattttgttg aacaggctgc ttgatggtct ggacggtgcg ctggcgcggc gcagagggct
    14701 taccgacgcg ggcggctttc tcgatatttc tctcgatttt cttttttacg cgctggtgcc
    14761 ttttgggttt atcctcgccg caccggagca aaacgccctg gtaggcggct ggctgctgtt
    14821 tgcgtttatc ggcacgggca gcagttttct cgcttttgct gcgctggcgg cgaaacatca
    14881 gattgataat cctggttatg cgcataaatc gttttattat ctgggcgggt taaccgaagg
    14941 cagcgagacg attttgctgt ttgttctgag ttgcctcttt ccggcctgtt ttccgtggtt
    15001 tgcgtgggta tttggtgcgt tgtgctggat gacgaccttt acgcgggtgt ggagtgggta
    15061 tctgacgctg aagtcgctcc agcgtcagta attgcaatgt tatttactgc tgtccgggcc
    15121 gcgttcaccg gttgctatcg gattttttgg atcgctgctc cattcgtacc agccaccgtc
    15181 gtaaacggag acgttattcc agcccattgc gcgtgcgtac ataaaggttt ctgacgcgcg
    15241 ccagccagtg ccgcagtaga atgaaacttg ctgatctggt ttgatattcc atgctttcca
    15301 catagcggtt atatcatcgg cgctgcgcat ggtgccatcc gggttatgga aatcttccat
    15361 atgcgtcgag tcgctacctg cgtgtcccca acgtgctccg gctatttcac cttttggttt
    15421 aatatagctg taaccgctgg tcgtaccgat aaattctggc cacgaacgaa tgctcaccag
    15481 cgatgcatcc tggcgatgca gcagtccacg cgcttgttcc atatcaagca tcagttgcgg
    15541 ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc actttcggtg gcgttccgcg
    15601 ctcaacgggc agacccgcgt cggaccaggt ttgccagcct ccatccagca ggcgcacatc
    15661 tttcacgccg gcataaagca taatttgcgc cacacgcgct gcagcgtata cgtcacgccc
    15721 gtacagaatg accgtggtgt catggcgaat gccgtgtttt gccagcatcg ctttcagttg
    15781 ttcatcagaa actttgttcc acagcggttc gctttccact tcgttggtgt cgatgtagtc
    15841 agcgccggga atatggctga taaggtaaaa ttttggagcg ccccaggccg cttcaatgac
    15901 tttccagtca ccggcaggtt tcgccgtaac ctctttacct tgttgcaggt catgcagcca
    15961 ctgcggatat accagctgtt caaagtgcgg cagtttttgc agacgggaag gttcgcttag
    16021 cgcgtcacta aggatggaga tatgcgtaaa acctgctttt tgcagtcgcg ttttgacggc
    16081 gtcgacatct ttgtcattac cgtacagcgc caccggggca tcggctttca ggttatgttg
    16141 cttgatccac tcgttgagct gttcggtgct cattttgtcg agccagctag cagagaggtt
    16201 taaggcggca ggttcatgac cggaagggcc atttaaggtt tgtggccagc cgttataaaa
    16261 cgcgctgggg cgagtatcaa tcgctttgcc attttgttgt tgaagctggt caagtgtaag
    16321 aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc agccctaaag ccattgccag
    16381 cgcggtcatt tgagaaacac gtttcatcga aaatcccgac attgtagttt tgagagcgcc
    16441 attctgaact gacgcatcgg gaaaaacttt gcgttatcgc agtttgttgt aattttctga
    16501 ccactgcgcc atatccagaa tggggctatc aggaggaaca tcctgaagat cgtgcgtcac
    16561 ctgcacaaca gggatcgcca tggcgcgaat ttcgctgaac acccaatggc gaaaattatc
    16621 gcgcagggcc acatcaagac ggctgaaggg ctcatccagt agcaacgctt ttggctgagc
    16681 gagaagggcg cgcagcagag caacgcgcgc tcgctgaccg ccagacaaag tggcaggatc
    16741 ctgatggaac gctccgtcaa ggcctgaacg ctcaagtgca tcattcacgg cattacgtcg
    16801 ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa ttttgcccga cgctgaactg
    16861 gtcaaataac agtgcatcct ggaaaagaat gccaatctga cgctgtgcgg tgggtagcat
    16921 gtcaatccgt tgctcattaa gccatagctc ccctgtacaa gaaaactgtc cggccagcgc
    16981 accaatcatc cacgaaaaca gagtggattt cccgcagccg gatggtccca ttaacgtgac
    17041 aatgtcacct ttatctactg aaaagttaac gtttgtcagc aagcggcttt ctggtaaacg
    17101 tagcgaaaca tttttcacgc agagcattag cggagtcctt gtctgacata acctacccat
    17161 tttgcgacta acgcggtcag ggcaaaaata ataagcgata acagcagttg ccataaagcc
    17221 tgggcggcga gaataccgtt gctgccgccg ctgcttaatg ccaccgcttc agtggtgagc
    17281 gtcggaaaac gccccgcgcc cagccacagc gtcggcatat actgcgcaat actgactgaa
    17341 aatcccaccg cgaaggcaat cagcgcaggg cgcaacatga gtgggcattt tacgtaaaag
    17401 aagattttgg cccgcgacca gcccagtgtt tgcgcaatca aaattaaccg tgaatcaatg
    17461 cgctgccagg cgggttgcag gataaacagc atccacggca ttacccacag cagatgcccc
    17521 cagaccaccg ccgtccagct tccatccagg ttcagccata gcgccagcgt gtactggccc
    17581 gccaccagag gcagagcggg taataaaatg ggcaaccata gccacaactg gcgacgctgt
    17641 ggcccccatt cctgccacag cagtaacagg agcaaagcga tgaatgccgc caccagcccc
    17701 attgtcaggc tgttgatgag cgcttcacta ttgatcgtcg actgatccgc gaggatcgcc
    17761 agcagaacca cacacagcac accggttaag ggtaaaaaac tcgccagcgt attgcctggc
    17821 aataaaggcg tggcaggctt acgaacgcca tctacgcggg gaatagtacg ccgccagcca
    17881 cgccatagca gatagcccag caaaacgtag gcggcgagta acagtatcag cagcaggctg
    17941 gcgagtgcgc ctttcgtttg ttgatcggcg tcgccctgag ttaaccactg ccagctaatt
    18001 accgccagcg tcgggggatt acctggccca agaataatcg ccacatccac gaccgacagc
    18061 gaccaggcga caatcgccag catcgccatt gccagcgcag acgcgacgga gggcaacagc
    18121 agccagttca ggcattgcca gcggctgtag cccagcgaat ccagcacaat gagttgctgc
    18181 aacagccgtt tttcgctcaa tactgccgct aagatccaca gcagaaatgc gctttctttc
    18241 accgccaggg tgaggccgag cccaataccc aatttgtcca ttggcggagt gaaatacggg
    18301 aaatagtcat aaagcagccc tccgtcagca aagagcagaa gggcgctggt ggcaaaagcc
    18361 acatggggaa tggcgagcag ccacggcagc cgggcgcaca tacgctgcca tttcggccct
    18421 ggccacagcg ccacaatcac cagcagggca atcaacaacg ccccgaccgc cgcaatggtt
    18481 gtcgacacca gcgtcgccag taatgcctgc ggtaactgtg gatcggcaaa taacgcctgc
    18541 cagtgcgtca acgacaaagc aagcgatatc agcgtgagag ctgccgggat cagcggtgca
    18601 taaatcaccg ccatcatcgc ccacagcaaa aagattaatg cgtaccgtaa cggtgtagcc
    18661 attcttgttc cagcgcattt acccaacctg cgtgcggttc agccagtacc ggcggcagat
    18721 cctgcggcat tctcgattgc aatgtttcgc gctgcccgtc aggcagtttt tgcggatcga
    18781 gaacagaagg atcgccccag acagcgggat ctgctttacg cagttgcgca tcgggtgaga
    18841 gcaggaaatt ggcaactacc ttcgccgcag cactggcatt cgcgttggca ggaatggtga
    18901 tgaaatggac gttgccaatc atcccctcgc gaaaaccaaa actgtaactg cttgcaggca
    18961 aatcgccgct ggcgattttt tgctgcgcat gtgcagggtt aaaggtcagc gacaggcgca
    19021 atgtgttagc tttcagcaga gcatccatcc gcgcgggcga aggcgggaaa tcctttcctt
    19081 cgcgccacaa atacggatgc agtgcatcaa gatattgcca caagggcgca gtgacgcggg
    19141 cgaaagtcgc atcgtccggc gcttctttta atgctgtggg atctggcgtt agcataatca
    19201 gcaactgttc aagaaacgcc gtaccggtaa agtccggtgg gcgcggatag gtaaccgtgc
    19261 cgggattggc tttagcaaac tccagtaagg cttgcggcgt ttgtggtggc tgtggcgtaa
    19321 catcgcggcg ggcgataaac gtcagttgtg cgccgcccca gggcgattca gccccttctg
    19381 taggcactga aaaatcttcc cgcaccggaa gctgtgtgtc gacatagcgc cagttgggca
    19441 gagtctccgc ccagcccgtt tgtaataaat tggcctcttt taaggtgcgg aagttttcgc
    19501 cgttcaccca gagcagatcc accgaaccgc ctgttttgcg tccggcagca gcttcggtct
    19561 gaatgcgctt caccgcgtct gcggcatccg cgagacgaac aatcttcagg tttatagcgt
    19621 aatgggtttt catctcgccg ctaacccagt cgagatagcg gttaattgcg gtatcgccgc
    19681 cccaggcgtt aaaccagacg gtttgtcctt tggcctcatt ttttatttcc tgccagtcac
    19741 tggcatgtgt tgccaaagaa aacagcgata acaatcccag caaccaccca caatggcgca
    19801 tcttctctcc ttaagaacgt ttttcaggaa tacggcacag cccctgagcg ggaatgtcca
    19861 tggcagaatt aaaacgggct gacagatttt gcaggcctat caatgccgtc agttcggtga
    19921 gcgcctgagc gtcaaaatgc gtagccagtc gggcgcgaag ggcatcatcg accgttggcg
    19981 gcgttacgct tgcggcttcg gcgtactcca gcgccagccg ttcttcatcg ctaaagagcg
    20041 ggctttgccg ccagtcagcc actgccagca gtttatcggt gctgccggta cgctcggcga
    20101 ctttcatact ggtgatatcc acgcaaaact cacacaggca catttgcgca atgcgcgcgc
    20161 tgacaagcga tcgtactacc ggatcgagcg gtgagcgttt gcgctccagc cagccgacaa
    20221 acatcgacac cagataaaag ataaacggga tccgccccca ccagcgaatg gggcttagca
    20281 cctcgccgta atggcgacga tgcagccacg cctgcggacg taaaatgaaa ggaattttgc
    20341 taagcggcgg taaacccatc aggcattcct cttctggcgc tcaagccata ttttcttcgc
    20401 cataaaaatc accacggtca gggcaaacag aataaacagt ccggtgacaa accagaaagt
    20461 accgcctgtt aacatgctgc ccgcccagga ataaacaata gtcgccggta attgaccaag
    20521 cccggtggcg ataaaaaacg agcgaaaacg tattgaagtc aaaccggcag catagctgat
    20581 tggatcgaaa gggacaaaag gcaataaccg acataccagg atggtgtgtt tgccgtaacg
    20641 agtgaaaaag ccgtccatac tgtcaagtac ggttttgccg gttaattttt ccaccacttc
    20701 gcggcccatc actctggcga taaaaaagca cagtgccgcg ccggccatcg aactggtcca
    20761 cgacagtaag ccgccccaga acgcaccaaa cagcgacgca ttggcaaagg tgatcaaaaa
    20821 cgcaggtagc ggtgcagcaa tggcctgtaa aatcatcaac aagaacgaga caactgccgc
    20881 tagtgcgccg taagactgaa taaagcgttc tataccctgt tggtccacgg cggcaaacgc
    20941 cgcaacgctg cgattgataa actcatggac gccgggaagc agcgcccatg ccagcagcac
    21001 agcgaacaac aggatgatga gggttatacg gtagtaccag attttacgcg attgcatctt
    21061 catcatccgt tatcctgctt tattcggtga ttttaaaggt aaccgttgcc agcgtgttat
    21121 ccgccgggag aactgaggca ttgcctttga atttcacttc gccgcgtttt tccaccgcac
    21181 cataagtgta tgttgcattg ctgacgatgc cgaccgggca gctatccaga cacaccaggc
    21241 aacccgtttt cttctcttct gctgccgtta aattaccgcc aaagcgcatg ttcagtttct
    21301 tgccattact gtcaacaatc acttcatcga aggaagacgc ttttgccgcc ccttgccagt
    21361 tgaccgaaat atccagtttg ctgcctgtga catgagtcgt ttctttatta tccatcgtca
    21421 tgttttcgcc cggcgtacca cctgcctctt tcagggcttc ataaaatgct ttcggggtcg
    21481 cataacccat aaacagcgat ttatgtccgt tggagccatc tttaaacaca ataccgtgac
    21541 gagtgtcgtc ggtgaggaaa cgaccattaa tttgcaccag catggttacc gtatggttat
    21601 tggcatcgac acgcagcggt tggctgttcg aaagtccgtc gtattccact tttgcagccg
    21661 cattctcttt ttgatcgcag ccgctaagcc cagcaacagc cagtaagcag agtgcagcca
    21721 gtcctttttt ccatgacact gaataatgtt gcaacatcgt tctatccttc attttttggg
    21781 tgagaaagtg ggctgcggcg agaagcagac agatccacat gtttgtgtcg ggcgtagagt
    21841 tttgcgagct ggacgagaat aaacagcgcc aggcccgcca gacagagttg taaaataaag
    21901 cgcagcgtaa tgccttcatt ggcgagatcg cttgccatca cggtataaat aacaataccg
    21961 ggcagggtcg tgagtgccga aataagggta taaggccaga aggcgattgt ggttaatccg
    22021 taagcgtaat tttgaatatt gtaaggaaac aacgggatta agcgggtcag aataagaaaa
    22081 tcaataccgt tacgcgcaat gcctttttca atggcctgga aggtatggct atggccaacg
    22141 tatttcagca gtaaatcacg tcccagccag cgcgccagca ggaatgagca cgaggaggcc
    22201 agcgtggcgg caattaatga tagtagtgtc cctaagagcg ggccaaaaac tattcctccc
    22261 gcgatcacca gtatgcttcc tggtaacaga aagagggtgg caatgatgaa taacagaata
    22321 tagaggctat agccgaacaa tccgctctgg cgaatgagtg tctgtaagtg gggtaaatcg
    22381 gtgagcagat cgaataaacc gaaagcgtgg atagcgtaaa tgaccagcgc aaaaataaga
    22441 caggcaaaaa gaaatttacg ctcagcgttc atcgtggagt ttcctatatc aaacttgata
    22501 atagagaagt ggtcagacaa ctaaaaacgc agtgacggta taacaaagga cggcaggcaa
    22561 caaatcatta ctgacagttt gttgattttt ctcaaggtta attctcctga cccagtttga
    22621 gccaggagat cagctaaatt agcggcggaa ggtcgcccag acgggggcgt gatcggacgg
    22681 tttttccatg ctgcgaattt catagtcgat gccggtttct acgcaacatt ctgccagcgg
    22741 ttggctggcg agcagcaggt cgatgcgcag accgcgatta tcgtcaaaac cttttgagcg
    22801 gtaatcaaac catgagaaac gatctgctgt ttgcggattc gcatggcgga aggtatcgac
    22861 caacccccag ctcatcagcc tctccatcca ttcgcgttcc tccggaagga aagaacattt
    22921 tccggtacgc agccagcgct tacggttctc ttcgccaatg ccaatatcca ggtcgccagg
    22981 actaatattc atatcgccca taatcagcac cggattttca cgcttgagtt cggtttccag
    23041 gtagttttgc agattctgat aaaactgcgc ctttgccggg aattttatcg gatggtcgcg
    23101 gctttcaccc tgcgggaagt aaccgttgat cacggtgaca ttgcccagcg gcgaggggat
    23161 ttccgccata ataatccgcc gttgcgcctc ttcgtcgtca ccaggaaagc cgcgacgcac
    23221 ggcaatcggc gtctctttgg tcagcagcgc cacgccataa tggcctttct gcccgtgata
    23281 aaacacgttg tagccgagct tcgccacctc ttcgagcgga aacatatcgt catgaacttt
    23341 tgtctcctgc aggccaatca catccggttg gtgcttttcg acgatggctt caagctggtg
    23401 aggtctggcg cgcaggccgt tgatattaaa agagacaaat ttcatagtcg ctgccattta
    23461 ttcagataaa gagtgcgtgg atggtagcag aatttcgctt tgcttaccgc ctgttgttaa
    23521 gtgatttgat cgatggttgt taatgatgtc aacgatggcg caaaaaatgc ccgcttttgg
    23581 tgcgcgttgc gtcagaatgg cgcagtaatt tccagtaaat ttcg
//
LOCUS       DAZGJT010000075        23437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZGJT010000075 DAZGJT010000000
VERSION     DAZGJT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.075"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..646)
                     /locus_tag="ACHKFK_003212"
     CDS             complement(<1..646)
                     /locus_tag="ACHKFK_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO9053486.1"
                     /translation="MSQLDSNAIKEIVKLTTTAFSGENLPLTECPVALLPDNVNIESL
                     ERFMTERFRFRGVMTTTSIDDFVEYSKGYADECSRCFINAETMKAVTVFNIGTLEQPG
                     HADNKALLELKATSPYRALRDVDGKKQLQKSLAEWLEDWADFLTAYDSDGNVLDIKQA
                     ISAVRRLTIDAKRSAEYEEQNFSGSRSVMESVEAKSKEIMPATFRFECIPYEGLG"
     gene            complement(710..1057)
                     /locus_tag="ACHKFK_003213"
     CDS             complement(710..1057)
                     /locus_tag="ACHKFK_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053487.1"
                     /translation="MNQHQTDVNVFINDLDGGVFVNKLGAVLSEVAFGVNSTNKKGKV
                     CVEFELSSLDENRVSVSHKLKFTRPTMRGSKSEEDTTNTPMFVNKGGELTLFQKDQGQ
                     LFDKQGQHDAVLR"
     gene            complement(1132..1719)
                     /locus_tag="ACHKFK_003214"
     CDS             complement(1132..1719)
                     /locus_tag="ACHKFK_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF669 domain-containing protein"
                     /protein_id="HFO9053488.1"
                     /translation="MDDVIFTYNEESALTAGQGGFITETGAHIINITEAELKQSEKGA
                     RFIEFSGESDDGRKIQYLSVCVQKNDGTENKFGASIIHAMMGCTGIGQLTQHMVSVSK
                     YVAPEFHGKKIGLVLQKVLTTNRKTGADSYQMEIRIPFIAETGQTLKEKAEGKKPETV
                     ANMVSTLKDKDNRSKNANPNHTDDPGYWQYGGDNF"
     gene            complement(1719..2411)
                     /locus_tag="ACHKFK_003215"
     CDS             complement(1719..2411)
                     /locus_tag="ACHKFK_003215"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9053489.1"
                     /translation="MTPEIILQRTGVDITSLDQGDDGWHKLRLGVITASEVHNVIAKP
                     RSGSKWPDTKMSYFHTLLAEVCTGVAPEVNAKSLAWGKQYEDDARALFEFIADVTVAE
                     TPIIFRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFNAIKPAYMAQVQF
                     SMWVTDKDAWYFANYDPRMKREGLHYVVVERDEKYMASFDEMVPEFIDKMDEALAEIG
                     FVFGEQWGGVNN"
     gene            complement(2408..3358)
                     /locus_tag="ACHKFK_003216"
     CDS             complement(2408..3358)
                     /locus_tag="ACHKFK_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000459722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO9053490.1"
                     /translation="MFDIVEFVKQQERFFCEALTEPTLTWAKESQFAIQQFQKNAFLA
                     DTARANLPSAQNAIINVAAIGITLNPASKLAYLVPRKKAVCLDISYMGLLHLAQVTGA
                     IQWGQCKLVYEKDIYESNGIDCAPTHKYNPFVDRGARIGGYCVVKTSEGDYLTEEMSN
                     REIEVIRACSKAGNNGGSSPWDSFPDEMARKAIVKRASKYWPRRDRLDTAIDYLNTQA
                     GEGIILNADHIPERDVTPASDEIINEITQAITEINKKWDDLLPVCSKTFRRTIASHED
                     LTQEEAVKTLDFVKKKAARNKATAEAKVHATTENNSEAVS"
     gene            complement(3375..3656)
                     /gene="gamL"
                     /locus_tag="ACHKFK_003217"
     CDS             complement(3375..3656)
                     /gene="gamL"
                     /locus_tag="ACHKFK_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host nuclease inhibitor GamL"
                     /protein_id="HFO9053491.1"
                     /translation="MNAYLTCDRIEERRWINQQIQDEKDKWIDDRSQELISMFPDKPL
                     LMSSLFLPKEAQLALTGEKAEEAYNDYISAIAYARAEEEWERKFSPCPF"
     gene            complement(3677..3958)
                     /locus_tag="ACHKFK_003218"
     CDS             complement(3677..3958)
                     /locus_tag="ACHKFK_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO9053492.1"
                     /translation="MLNQKININVKSVVTPAGTIMGEVFMDDKIIAYFVVLSDEAISV
                     IDTEGNVMFIAEHPEDIALQAAAYFFAKEQEEECNCPVCQLSRQINLMH"
     gene            complement(3970..4182)
                     /locus_tag="ACHKFK_003219"
     CDS             complement(3970..4182)
                     /locus_tag="ACHKFK_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9053493.1"
                     /translation="MLKPHYGTALVSREDVKPGTAILYNGRYYMASANVNNALYAHSL
                     IEKIRIISDAIEVYLNNKGQPLISPA"
     gene            complement(4253..4927)
                     /locus_tag="ACHKFK_003220"
     CDS             complement(4253..4927)
                     /locus_tag="ACHKFK_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO9053494.1"
                     /translation="MKTISVESLSIISFSNIPVVTTELLASLYGTEPDYIRKNFNRNS
                     GRFVIGKHYFLLENEELREFKHSMSLRPSVKIARNVRSLILWTERGAARHAKMLETDR
                     AWEVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSDNHPYATMNYVDRKVISLDA
                     SVLFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRKLLDISRTSEKSSRF
                     GFNVNL"
     gene            complement(5183..5956)
                     /locus_tag="ACHKFK_003221"
     CDS             complement(5183..5956)
                     /locus_tag="ACHKFK_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9053495.1"
                     /translation="MGPTSVGLVPLIASSPNPVQSTASELRTSCGGSQSPIKEAAIMA
                     TIPALSQPEITITNGRVVTTSIAIAEFFDKRHDDVLKKIRLMDCSTEFNAHNFADVTY
                     TDAKGEQRPMYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEKQLWEQKQLALSS
                     PAMNINFPLSWFSEHHPYATMNYVDRKVISLDASVLFDMPSPTMRILNELHSKGYNVD
                     AAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGFSLSL"
     gene            complement(6585..7538)
                     /locus_tag="ACHKFK_003222"
     CDS             complement(6585..7538)
                     /locus_tag="ACHKFK_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BsuBI/PstI family type II restriction
                     endonuclease"
                     /protein_id="HFO9053496.1"
                     /translation="MNNQNDYIEAAQQIIASLGLPRAQQNERSALCLLALLNLTPGKA
                     WADAENPLVGITPIMNWVREHYGKVYAPNTRETFRRQSMHQFCAAGVALYNPDKPDRP
                     VNSPKAVYQIEPAALSMLRTFGSPAWHDSLATYLAERETLVTRYAKEREQNRIPVEIA
                     AGQQITLSPGEHSELIRAIIEDFAPRFAPGSVLVYAGDTGEKWGYFDAPLLAGLGVDV
                     DSHGKMPDVVLHFTAKNWLLLVESVTSHGPVDGKRHAELARLFAGSTAGLVYVTAFPN
                     RSIMGRYLGEIAWETEVWVADAPSHLIHFNGVRFLGPYSTE"
     gene            complement(7535..9004)
                     /locus_tag="ACHKFK_003223"
     CDS             complement(7535..9004)
                     /locus_tag="ACHKFK_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HFO9053497.1"
                     /translation="MLQKLDVADSVRRELSPRTARKHKAEFGQFMTPSGVARFMASLF
                     PPSTMKTCRLLDAGAGVGALSCAFLDRWLVGEFGFESVEATAYEIDKKLCGHLAKHLT
                     GYRRVTPRIIEGDYIELATAEGLQDRGYTHAILNPPYKKINSQSAHRQALRTVGIETV
                     NLYSAFVALAVGEVAPGGQIVAIIPRSFCNGPYYRPFRNFILERAAIRHIHLFESRSK
                     AFRDDDVLQENIIIRLERGGRQEAVTVTTSTDDSFFDLVTYEHPFDQIVYPDDSERFI
                     HVPTTLEKSTIELSSAVQCSLADIGVKVSTGPIVDFRLKAHLRSMPEEGTVPLIYPSH
                     LSMSSTVWPVEGLKKPNAIMRNDETEKWLYPNGFYCVVRRFSSKEEKRRVVASVVDPA
                     TFSEYSVLGFENHMNVFHENKHGLPEALARGLAVFLNTTAVDKYFRRFNGHTQVNATD
                     LKMIKYPSRDTLIELGKWAMQQETLTQEHIDAKLGALTA"
     gene            complement(9099..9812)
                     /locus_tag="ACHKFK_003224"
     CDS             complement(9099..9812)
                     /locus_tag="ACHKFK_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9053498.1"
                     /translation="MNMKKKPLTPEQLEDAKRLKSIFNAKKKELGLSQESLAYELGVT
                     QSAVNQLMAGINAINASHAAQLAKILNVKVGDFSPSLAKSIAEMALAIEEPLTRVPAY
                     EYPLLSCVQAGAFSMDDISYTAKDAIKWISTTTKASDRSFWLEVKGHSMTAPQGGKPS
                     FPEGMLILVDPEREIEDGDFCVARMNGDEFTFKRFIRESGKAYLEPLNPRFDMIECNE
                     NCQFVGKVIKSQWNDETFD"
     gene            9908..10111
                     /locus_tag="ACHKFK_003225"
     CDS             9908..10111
                     /locus_tag="ACHKFK_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9053499.1"
                     /translation="MAMKKETLADYVYRFGQKKAAKDFGVAQSAISKALLVGREIYVK
                     TFDDGTVEAEEVRPFPAFVRGDD"
     gene            10166..10435
                     /locus_tag="ACHKFK_003226"
     CDS             10166..10435
                     /locus_tag="ACHKFK_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053500.1"
                     /translation="MPEQYCDADRDWIQEQLLQLDPTTRVKIAAEYARVYQEEWDKEP
                     VSFRKGNRARRSANTRLRVYVQKYARASRGWMLSPVAVRKFPPTP"
     gene            10643..11020
                     /locus_tag="ACHKFK_003227"
     CDS             10643..11020
                     /locus_tag="ACHKFK_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053501.1"
                     /translation="MSAATKSALIRTLSTVPLRTEERYSFLADVVTILESQGMHVASN
                     VTVRIDGRNFRVDILATAKTGGSVAIEIDRSSPRPRSVMKLRELARRGTEGFVLLRMP
                     KKLTSYSDAGIDIIPANGKGASC"
     gene            11014..12534
                     /locus_tag="ACHKFK_003228"
     CDS             11014..12534
                     /locus_tag="ACHKFK_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO9053502.1"
                     /translation="MLKITPNFAQERGLNQLRHQWKQHRTYLMYAPTGSGKTGLAAFV
                     TAGMVERGMRVMFVCPYTILLNQTAERFTEYGLPWEEISFVWRDHPNYDPSRLIQIAS
                     ADTLIRREFPDNIDLLIIDEAHMKRRALLEVIRDKDIRVLGLSGTPFAAWMGKYYECL
                     IKPTTIRELIQRGDLSDYEFFAPSMPDLAGVKTSNTVFGRDYNEEQLASIMGSSDLVG
                     DIVSNWLENGEDLPTICFCVNVAHANFVTREFLQSGIGAEVMTADTPHDERQDIIRRF
                     EEGATKIIVNVGVLVAGFDSDVRCLIYARPTKSEIRWLQCIGRALRTASGKKRALIFD
                     HSGTVHRLGFPEDIEYDELPGKNDGMKASAGGSEVKAEKLPRECPKCHFMKPAGVHMC
                     PKCGFRPLGGDDVATDRDRKLSRVNKGKREYTREEKQRWWSEIKGYQNYRNATGKPLS
                     DGWCAHTYKEKFGVWPKGFSNAPLQTSVEVYNFIKSKTIAYAKGRKKAMTGGQHAN"
     gene            12524..13495
                     /locus_tag="ACHKFK_003229"
     CDS             12524..13495
                     /locus_tag="ACHKFK_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9053503.1"
                     /translation="MRTRDAAVGHWSRIFEYYGMPPVTGLKHYSGPCPICGARGKFRC
                     DDKDGSGSWICVCGHGDGMNLLQLATGKPWVTLCDEIDRLIGNTWKREKVSQPVTEIS
                     RKRKLVMDKFAGLPCLRGTTGEAYLQGRGILQLPIESVRFCDRQIASGREYQAIYAIA
                     TDDKGSLCYLHRTLLDGDRKANVEAAKKMTALQELPGLQHAKSVAIRLYPVSSTLGIA
                     EGIETALSCRQIFRCNVWSTMNSGFMEKFIAPPGVNHLIIFADNDAHGAGLAAAFKCG
                     HKNLMSCNDVEKVSIRWPDLPDFNDMLIQGCEAREHVLTRKFKAEAA"
     gene            13495..13944
                     /locus_tag="ACHKFK_003230"
     CDS             13495..13944
                     /locus_tag="ACHKFK_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFO9053504.1"
                     /translation="MEIEMIKAANGVFVPAYERDLPRLAKFKNGELYTLEAKLTRNPS
                     FHRKMFAFLNFCFQYWCAEHAGYEFSDEATQFDEFRKNLTILAGFYDVVTTIRGEVRY
                     RAKSLSYANMDQDEFERCYNAMINAALKHVFGRSNNPELNNRLLSFF"
     gene            13952..14515
                     /locus_tag="ACHKFK_003231"
     CDS             13952..14515
                     /locus_tag="ACHKFK_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9053505.1"
                     /translation="MKQRKPKKCKVCGSSFVPFRSYLKVCCGQCALELVRKEKAIASA
                     KEQADKLKARRRDLQPRSYWIKQAQQAVNAYIRERERHLPCVSCGTFDSAQWDAGHYR
                     TTAAAPQLRFDERNIHKQCVVCNQYKSGNLVPYRVELINRIGQEAVDEIESNHSRHRW
                     TVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            14512..14706
                     /locus_tag="ACHKFK_003232"
     CDS             14512..14706
                     /locus_tag="ACHKFK_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO9053506.1"
                     /translation="MTFTVKTIPDMLVEAYGNQTEVARILNCNRATVRKYIGDKEGKR
                     HAIVNGVLMVHRGWGKDTDA"
     gene            14699..15133
                     /locus_tag="ACHKFK_003233"
     CDS             14699..15133
                     /locus_tag="ACHKFK_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9053507.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKRLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            15640..16588
                     /gene="stxA1a"
                     /locus_tag="ACHKFK_003234"
                     /pseudo
     CDS             15640..16588
                     /gene="stxA1a"
                     /locus_tag="ACHKFK_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
     gene            16598..16867
                     /gene="stxB1a"
                     /locus_tag="ACHKFK_003235"
     CDS             16598..16867
                     /gene="stxB1a"
                     /locus_tag="ACHKFK_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO9053508.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            17372..19312
                     /locus_tag="ACHKFK_003236"
     CDS             17372..19312
                     /locus_tag="ACHKFK_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9053509.1"
                     /translation="MAFKHYDVVRAASPSDLAERITQKLKEGWQPYGSALISTAGYGA
                     EFIQPVVSEGELPSLAESGNHPHVSAKPEAAPEYYYVIALAGQSNSMSYGEGLPLPET
                     YDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKAELSKGQYG
                     TVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWGVD
                     KPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLA
                     DMAGQCVGGSAGGVPWICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDEN
                     GVNVPTNKPEEDPDIPGIGYYGSKWRDSSATWTSQDRASHFSSWARRGIISDRLATAI
                     LRHAGRVALNVGAPSTVSEVRPSSPSGAEVTGVTTLLSYLASESEGSLKVQGWSASGG
                     RAEVVSDAEGTGGKAVKVTKEAGKSSWVLEYAAGNGAALLQKGGQIRCRFKASGALAA
                     NQYVMAFYWPVSSLPQGVVLTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLG
                     SFGAFDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFSADKLHVTDITK
                     SATYPVLIDSIAVEVNNADAAA"
     gene            19448..19627
                     /locus_tag="ACHKFK_003237"
     CDS             19448..19627
                     /locus_tag="ACHKFK_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9053510.1"
                     /translation="MTFLNQLMLYFCTVVCVLYLLSGGYRAMHDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLV"
     gene            19668..19913
                     /locus_tag="ACHKFK_003238"
     CDS             19668..19913
                     /locus_tag="ACHKFK_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9053511.1"
                     /translation="MSEITSLVTAEAVKDVLRSEEVLSALKQKLRHNLEARLDAEVDA
                     ILDELLGAPAAPEPEGIAGEGSASDSGDPTPDSDMMM"
     gene            19991..20206
                     /locus_tag="ACHKFK_003239"
     CDS             19991..20206
                     /locus_tag="ACHKFK_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9053512.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            20211..20744
                     /locus_tag="ACHKFK_003240"
     CDS             20211..20744
                     /locus_tag="ACHKFK_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9053513.1"
                     /translation="MSRKFRYGLSAVVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAIERDKALAWVAKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRNRSNNCYGQ
                     VSRRDQESALACWSIDR"
     gene            20965..21078
                     /locus_tag="ACHKFK_003241"
     CDS             20965..21078
                     /locus_tag="ACHKFK_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053514.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            21080..21538
                     /locus_tag="ACHKFK_003242"
     CDS             21080..21538
                     /locus_tag="ACHKFK_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9053515.1"
                     /translation="MNRVLYGVLIVLLLGCGSLWLATDHYRDNAITYKEQRDDAKTKL
                     SQANATIADIQQRQRDVAALDARYTRELADANATIESLRADVSAGRKRLQIAATCAKS
                     TTGASGMGDGESSGLTADAELNYYRLRSGIDKITAQVNYLQEYIRMQCLD"
     gene            complement(21619..22200)
                     /locus_tag="ACHKFK_003243"
     CDS             complement(21619..22200)
                     /locus_tag="ACHKFK_003243"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000934362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase"
                     /protein_id="HFO9053516.1"
                     /translation="MLNQTLTKSLLIFLLVIIVSVSIIYIARTPVRLDVNDVTEINQK
                     HPIIFLIRHGERCDRSQRICLSAHEGITVNGANKAQQYGDKFRRMFPYYSLYSTDTLR
                     TMQTATFFSGGKTATIPDISTCDDNAVNNILKISKSDHVTVIFTHNHCLSRIAKKMNG
                     WRFKPDYMGTLVLHRENHNLILDGHLKPNELTQ"
     gene            22803..>23437
                     /locus_tag="ACHKFK_003244"
     CDS             22803..>23437
                     /locus_tag="ACHKFK_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001086069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9053517.1"
                     /translation="MAKLDWKKLEQAFRREHVKTGIKLQDWCRQNNISYDTARRYIKL
                     RKHSPRNTEKSAQKNAQIDDQKSAQKSDGNSSHDEPSGDDGCDEKCANLAETKRTRGS
                     RLLPPSNAFSQRNTHAVRHRGYAKYLEADNLIDDAVGMELADELVFTRARALSVTGTL
                     KKMFADLKEAADVETRVALYDKILKAEQALDRNIARIESIERSLLTLDVLA"
BASE COUNT         5917 a         5240 c         6160 g         6120 t
ORIGIN      
        1 tgcccaggcc ctcgtatggg atgcattcaa agcggaaagt ggcaggcatg atttctttgc
       61 ttttagcctc aacggactcc atgactgagc ggctaccgct gaagttttgc tcttcatatt
      121 cagcactgcg ttttgcgtcg atggtaagac gacggacagc agagatcgcc tgtttgatat
      181 ccagcacatt accgtcgctg tcataggctg tgaggaagtc ggcccagtct tccagccatt
      241 cagcgagaga tttctggagt tgttttttgc cgtccacgtc acgtaatgcg cgatatggtg
      301 atgtggcttt cagttccagc agtgccttgt tgtcagcgtg tccaggttgc tccagagtgc
      361 cgatattgaa cacagtgact gctttcatcg tttccgcgtt aatgaagcag cgggagcact
      421 catcggcata ccctttgctg tattcaacaa agtcatcaat actggttgtg gtcatgactc
      481 cacggaaacg aaaacgttct gtcatgaatc gttccagact ttcgatattt acattgtccg
      541 gcaataacgc aacagggcat tccgtaagcg gcaaattttc accagaaaaa gcagtagtgg
      601 tgagttttac aatttcttta atggcattgc tatctaactg agacatgtat atttaccttt
      661 aatttatgta tttagggata atgcgcgagc gttttaaggc ggggattatt cagcgtaaaa
      721 cagcgtcatg ttggccctgt ttatcaaaaa gctgtccctg gtctttctgg aacaaagtaa
      781 gctcaccacc tttatttaca aacattggtg tgttagtcgt atcttcttct gatttactac
      841 cacgcattgt cgggcgtgtg aattttagtt tatgggaaac tgatacgcga ttttcatcaa
      901 gtgaagataa ttcgaattca acacatacct ttcctttttt gtttgtgctg tttacgccaa
      961 aggcaacttc acttaatacc gcaccaagtt tgttaacaaa taccccgccg tcgaggtcgt
     1021 taatgaaaac attaacatca gtttgatgct ggttcatatt aatatctccg tgtagaggtg
     1081 tgttaaggct tcagttgttc actgcattta aagcctggtt ttgtttgtga attaaaaatt
     1141 atcgccaccg tactgccagt aacccgggtc gtccgtgtga ttcgggtttg cgtttttgct
     1201 gcgattgtct ttgtctttga gagtggaaac catatttgct accgtttctg gtttctttcc
     1261 ttcagctttt tctttcagtg tctgccctgt ttctgcaatg aatggaatgc ggatttccat
     1321 ctggtaactg tctgcgccag tctttctgtt tgtggttaat actttctgga gcactaaccc
     1381 gattttcttt ccgtgaaact caggtgcaac atatttactg acggaaacca tatgttgcgt
     1441 taactgccca attcctgtgc atcccatcat ggcgtgaata atgctcgccc caaatttatt
     1501 ttcagtgccg tcattcttct gaacacaaac actgagatat tggattttcc gtccgtcgtc
     1561 ggattctcca gaaaactcaa tgaatcgggc acctttttct gattgcttga gttctgcttc
     1621 ggtgatgtta atgatatgcg cacccgtttc ggtaataaaa ccaccttgtc cggcggttag
     1681 tgctgattct tcattgtaag taaaaattac atcatccatt agttattaac ccccccccat
     1741 tgttctccaa atacaaaacc aatttccgcc agtgcttcgt ccattttgtc gatgaactcc
     1801 ggcaccatct catcaaaact cgccatgtac ttttcatccc gctcgaccac gacataatgc
     1861 aggccttcac gcttcatacg tgggtcgtag ttggcgaagt accaggcgtc cttgtctgta
     1921 acccacatgc tgaattgcac ctgggccatg taagcaggct tgatagcgtt aaagccgcca
     1981 agccggaact tcatgaagtc gcgggaagtg aaggggcatt taagctcaag accgttaccg
     2041 tcgctgcata aaccgtcggg agagcaggcg gtgcgcatgc tttcgtcacg aaaaattatt
     2101 ggcgtttccg cgacggtaac atccgcgata aattcgaaga gggcacgggc atcatcttcg
     2161 tattgctttc cccatgcgag cgacttagcg ttaacttccg gtgccacgcc agtacaaact
     2221 tcagccagca gggtgtggaa gtatgacatt tttgtatcag gccatttgct gcctgaacgt
     2281 ggtttggcta tgacgttgtg aacttctgaa gcagtgataa caccgagtct cagcttgtgc
     2341 catccatcat cgccctggtc gaggctggta atatccacgc cagttcgctg gagaataatt
     2401 tctggtgtca tgacacggcc tcgctgttat tttccgtggt ggcgtgaact ttcgcttccg
     2461 ccgtggcctt gtttctggca gctttctttt tgacaaaatc cagcgttttg acggcttctt
     2521 cctgcgtgag atcttcatgt gatgcaatcg tgcgacggaa tgttttggaa catacgggga
     2581 gaaggtcatc ccatttcttg ttgatttcgg tgattgcctg agtgatctca ttgataatct
     2641 catctgatgc gggagtgacg tcgcgctcag ggatgtgatc agcattcagg ataatacctt
     2701 caccggcctg agtgttcagg tagtcgatag ctgtatccag gcgatcgcga cggggccagt
     2761 atttgctggc gcgtttaaca atggctttgc gggccatttc atccgggaag ctatcccacg
     2821 ggctacttcc tccgttattt ccggctttgc tgcacgccct gatgacctcg atttccctgt
     2881 tgctcatctc ttcggtcaga tagtcgcctt cggatgtttt tacgacacaa taaccgccaa
     2941 tgcgtgcgcc cctgtctacg aatgggttgt atttgtgcgt gggggcgcag tcaataccgt
     3001 tggactcgta aatgtccttc tcgtaaacaa gtttgcattg cccccactga atggctcctg
     3061 tgacctgtgc cagatgcaga agccccatat aactgatatc caggcataca gccttttttc
     3121 gtgggaccag atacgccagc ttgctggccg ggttcagggt tatgccgatg gcggcaacat
     3181 tgatgatagc gttctgtgcg ctgggcagat ttgcccgtgc tgtgtcagcc agaaaggcat
     3241 ttttctggaa ttgctgaatt gcaaactgac tttccttcgc ccatgtcagc gtcggttcag
     3301 ttaatgcctc gcagaaaaag cgctcctgct gcttaacaaa ttcaacgata tcgaacattt
     3361 tttggtcctg aaaatcagaa aggacagggg gagaattttc tctcccattc ttcttccgcc
     3421 cgagcatagg cgatcgctga gatataatcg ttgtacgcct cttcagcttt ttcgccagtg
     3481 agtgccagtt gggcttcttt gggtaaaaaa aggctgctca taagcaatgg tttatcgggg
     3541 aacatgctga taagctcctg cgaccgatca tcaatccatt tatccttttc atcctgaatt
     3601 tgctgattaa tccagcgacg ctcctctatg cggtcgcagg tgaggtatgc gttcatggcg
     3661 gaactcctga ttccggttaa tgcattaaat taatttgtcg ggaaagctga catacagggc
     3721 agttacattc ttcctcctgc tccttagcga agaaatatgc agcggcctgt aatgcgatgt
     3781 cttctggatg ttctgcgata aacataacat tgccttccgt atcaataaca gaaatagcct
     3841 catcagacag gacgacaaaa taggcgatga ttttatcatc cataaaaact tctcccatta
     3901 tcgttcctgc tggagttacg acgcttttta cattgatatt tattttttga ttgagcatga
     3961 tatttccttt caggctggtg agattaacgg ttggccttta ttgttcaggt aaacttctat
     4021 tgcatctgag ataatgcgaa ttttttcaat cagtgaatga gcgtaaagtg cattattaac
     4081 gttcgctgac gccatgtaat aacgcccgtt gtaaagaatt gctgtgccgg gtttaacgtc
     4141 ctcgcgagaa actaatgcgg ttccgtagtg aggtttgagc atgacaaatc ctccggttaa
     4201 ttaatccaga tatttaattt aatccccgat atgtggtcgg ggatggggtt aattaaagat
     4261 taacgttgaa accaaagcgg gaagactttt ccgatgtgcg ggaaatatcc agcaatttac
     4321 ggcgcatttc ttccgtcaga tgtttgaagg cgttaaattc agcgacagct gcgtcgacgt
     4381 tatagccctt gctgtgtagc tcgttgagga tgcgcatagt tgggcttggc atatcgaaga
     4441 gcacggaagc atcaaggctg ataaccttgc gatcaacata gttcatcgtg gcgtagggat
     4501 ggttatctga gaaccacgag agagggtagt tgatagatag cgttgattct ggaagctgaa
     4561 gttgttctgg gattttttga ctgaaatagc agtcttccag tttttcgaac acttcccacg
     4621 cccgatcggt ttcgagcatt tttgcgtgac gggctgcgcc gcgttctgtc cagaggatga
     4681 gggagcgaac gttacgggcg attttcacag aaggtcttaa agacatgctg tgcttaaatt
     4741 cccgcaactc ctcattctca agtaaaaagt agtgcttacc gataacaaat cgtccagaat
     4801 tccgattgaa attttttcgg atgtaatctg gttctgtgcc atataagctg gcaagaagct
     4861 ctgtagtcac cacaggtata ttactaaatg aaattatgga gagggactcc acggagatgg
     4921 ttttcatgct ttgcgtccta cttgatattt tgatttaccc cttttgagag ggtgaccggg
     4981 cgctcaaaac cgcaagtagt cggcgggcat attcccattg ctggtattgt attagccgca
     5041 cgcccggtca taaaccaaga agactggaca caaaaaaacc gcatgtctgt cgggcgcggt
     5101 aaccgctact tgctaaggtg ttttgagcac catgaggcag aatatatccc cgttaatgca
     5161 gcattgtcaa acgatctgtt ctttaaagac ttaacgagaa gccttttcgg gaatttcgat
     5221 ctgacagtct tgaaatatcc tgtagcgtgc ggcgcatttc ttccgtcaga tgcttgaagg
     5281 cgttaaattc ggcgacagct gcatcgacgt tatagccctt gctgtgtagc tcgttgagga
     5341 tgcgcatagt tgggcttggc atatcgaaga gcacggaagc atcaaggctg ataaccttgc
     5401 gatcaacata gttcatcgtg gcgtaggggt gatgctctga aaaccacgac aaagggaaat
     5461 tgatattcat cgcaggagaa gacagggcca gttgtttctg ttcccatagt tgtttttcca
     5521 tgcgatcgaa ttcagcaatg taggcttcct tgaatgcagc tgcttttttg ccggtgaagc
     5581 ccatcaccag aaaaacgaag ccgtttttgg tgatttggta cattgggcgt tgttcgcctt
     5641 tggcgtcggt gtaggtaacg tccgcaaaat tgtgggcgtt aaattccgta gaacaatcca
     5701 tcaatcggat tttttttaaa acgtcgtcgt gccgcttgtc aaaaaattca gcaatagcaa
     5761 tagacgttgt gacaacgcga ccattggtaa tggtgatttc aggttgagaa agggcaggga
     5821 tagtagccat aatggcagcc tccttgattg gtgattgaga accaccgcag gaggtcctaa
     5881 gctcgctggc ggtggactgt acagggttag gactactggc aatcaaggga accagcccga
     5941 ccgaagtcgg ccccatacag cccaccattg ataagatgtg cgtgtatgtc gatacaaaaa
     6001 aagacgctgg cgcgtctgta tcgcctcgat tgtcagcggg gtcctaatcc cgacacccgt
     6061 tttataaggt gttcgccaaa tatagccccg acatcacacg cagtcaatac cgtcctttct
     6121 cggaaatgct ttggcggtgc cgcctgcacc ccaaaacatt ccctgtattg gtcagcgcca
     6181 acttcctgcc agtgttgccc gttctcacgc cgttctcgct ctcgcgcggg gatactctct
     6241 catcgaccgg atcgcacccg atgatacagc acgtttacgt gtaggggtct aaacaggtca
     6301 ttgacgctgt aaagctccag attgttaaag agcattttgc ggcgggttaa gtcgcgccgt
     6361 acgactgatt tatgtagccc tgtgtaaggg cgcgatgttt ctggcttgaa ataaatataa
     6421 cttgcggtga ttttcatgta aataccgttg gtgcttttta agggcgagaa attattactt
     6481 aactgatttt taaagtgatt tattttttaa gggggcagat aacaggaggg gatatgcaaa
     6541 gaaaaacccg gcgcatgggc cgggctattt atctggtcct gttctcattc agtagagtag
     6601 gggccaagaa atcgcactcc gttgaagtga ataagatgag aaggtgcatc agccacccat
     6661 acctctgttt cccatgcgat ttcaccaaga tatcgcccca tgatggagcg atttggaaaa
     6721 gcggtcacat agacaagtcc ggctgttgat ccggcaaaca gcctggcaag ctcagcatgc
     6781 cgcttcccat caaccggtcc atgactggtg acagactcaa ccagtagcag ccagtttttc
     6841 gcagtaaaat gtagcaccac atctggcatt ttaccgtgtg aatccacatc aacacctagc
     6901 ccggccagca gtggagcgtc gaagtagccc cacttttcgc cagtgtcgcc agcatagacc
     6961 agcacgctac ccggagcaaa gcgtggggcg aagtcctcta ttatggcacg gataagttcg
     7021 ctgtgctcgc cgggactgag ggttatttgc tggcccgcag caatttcaac agggatacga
     7081 ttctgttcgc gctccttagc atagcgggta accagcgttt cacgctcggc taaataggtt
     7141 gcaaggctat cgtgccatgc cgggctgccg aaagtgcgta gcatggacag agcagcaggt
     7201 tcgatctgat aaacagcctt cgggctgttc actgggcgat cgggcttgtc cggattgtag
     7261 agggctactc cagcagcgca gaactgatgc atagactggc ggcggaatgt ctcacgagta
     7321 ttgggtgcgt agaccttgcc gtagtgctcc cgaacccaat tcatgattgg tgtaatgccc
     7381 acaagcggat tttccgcgtc ggcccatgct tttcccggtg tgaggttcag gagtgccagc
     7441 aaacacaaag cagaacgctc attctgctgc gctctgggca ggcctaagga tgctataatt
     7501 tgctgagcgg cctcaatata atcattttgg ttgttcatgc agtcagagct cctagcttag
     7561 cgtcaatatg ttcttgcgtg agagtttctt gttgcatagc ccacttgcca agctctatca
     7621 gagtgtcacg gcttgggtac ttaatcatct tgaggtcggt tgcattgacc tgtgtatgcc
     7681 cgttgaagcg acggaaatac ttatccactg cggtagtgtt caggaacacg gccaagccac
     7741 gggccagcgc ctcgggtaac ccatgtttgt tctcatgaaa cacgttcatg tgattttcaa
     7801 atcccaacac tgaatactca ctgaaggtag caggatcaac gacgcttgct accactcggc
     7861 gcttttcctc cttcgacgag aaacgacgca ccacacaata aaaaccgttc gggtaaagcc
     7921 acttttctgt ctcgtcgttg cgcatgattg cgttgggttt cttcaaacct tccacaggcc
     7981 agacagtgct actcatactc aaatggctcg ggtagatcag gggaacagtt ccttcttctg
     8041 gcatgctgcg caaatgagct ttcagtcgga aatcaactat cggtccggtc gataccttca
     8101 cgccaatatc agccagcgaa cattgtacgg cagatgataa ctcaatggtg cttttctcga
     8161 gcgtggtcgg cacatgaata aaccgctccg aatcgtccgg gtacacaatc tggtcaaatg
     8221 ggtgttcgta ggtgactagg tcaaaaaagc tatcatcggt cgaagttgtg actgtaacag
     8281 cctcctgacg gcctccacgc tccaggcgga tgataatatt ttcctgcaac acatcgtcat
     8341 ccctgaaagc tttgctacgt gattcgaaca aatgaatatg gcggattgct gcccgctcaa
     8401 gtatgaaatt acggaacgga cggtaatacg gtccgttgca gaaactacgt gggattatgg
     8461 ccactatctg cccgccaggt gcaacctcac ctacagccag tgcaacaaag gcggaataca
     8521 gatttaccgt ctcgataccg acagtgcgaa gagcctgccg gtgggctgac tggctattga
     8581 tcttcttgta aggcggatta agtattgcat gggtataacc tcgatcctgt agaccttcgg
     8641 cggttgccag ctcaatataa tccccttcga tgatgcgggg cgtcactctc ctgtacccgg
     8701 ttaagtgctt cgcaaggtga ccacacagct ttttgtcgat ttcgtaggct gttgcctcga
     8761 cagactcaaa gccaaactcg ccaaccagcc aacggtcgag gaaggcgcaa gacagtgcgc
     8821 ctacaccagc gcctgcgtcc agtagacggc aagttttcat tgtgctcggt ggaaacaagg
     8881 aggccataaa acgggccacg ccagaaggcg tcatgaactg gccgaactct gctttgtgtt
     8941 tacgcgcggt acggggagac agctcgcgac gcacgctgtc tgcgacgtct agcttttgaa
     9001 gcataactaa tcctctttac gtggcatgat gatgccagcg tgcttttgac atcctccaca
     9061 ttcttcaggc gtgaaggatg tcaaatcact ctgtgagctc aatcaaaagt ctcatcattc
     9121 cactgcgatt tgatgacctt tccgacaaac tggcagtttt cgttacactc aatcatgtcg
     9181 aatcgtgggt tgagcggctc caggtacgct ttaccgctct cacgaatgaa tcgtttgaag
     9241 gtaaattcat cgccgttcat tcgggccacg caaaaatcgc catcctcgat ttcccgttca
     9301 gggtcaacga gtatcagcat accttcaggg aagctgggtt taccaccctg cggtgcggtc
     9361 attgaatgcc ccttgacctc cagccagaac gacctgtcac tggcttttgt ggtggtggag
     9421 atccatttaa tcgcatcttt tgcggtgtac gaaatatcat ccattgagaa tgcgccagct
     9481 tgtacgcagg agagtaacgg atattcgtaa gcggggacac gagttaatgg ctcttcaatt
     9541 gcgagcgcca tttctgcaat agattttgcc aggctgggac tgaaatctcc gacctttaca
     9601 ttcagtatct tagcaagctg tgctgcgtgg ctggcattta tggcgttgat gccagccatt
     9661 agctgattaa cagcgctttg ggtaacacct aattcatagg ctaatgattc ctgcgacagg
     9721 cctaactctt tctttttagc gttaaaaata cttttcagac gcttggcgtc ttccagttgc
     9781 tcaggggtta acggtttctt tttcatgttc ataccatatc acctttggtt atatgtaatg
     9841 aaatatctgc ggtgttgaca ttttaataac tgtgcgtaat gatatgggca ttttgaagga
     9901 gtcagttatg gcgatgaaaa aagagaccct ggctgattac gtatacaggt tcggccaaaa
     9961 gaaagccgct aaggactttg gcgtggcgca gagtgcaatc agtaaagcgc ttctcgtcgg
    10021 acgagaaata tacgtcaaaa cctttgatga cggcacagtt gaggcggaag aggttcgtcc
    10081 gttccctgcg ttcgtaagag gcgatgatta atcctggaca cacagcccat cggaggtgtg
    10141 agtgaacagg caaatcaaaa cggttatgcc cgagcagtac tgtgatgctg atcgggactg
    10201 gatacaggag caattgttac agctagaccc gaccacccgg gtaaaaattg cagcagagta
    10261 cgcgagagtg taccaggaag agtgggacaa ggagcctgta tcgttcagga agggcaacag
    10321 ggcaagacgg agcgccaaca cccggttacg ggtgtatgtc cagaagtacg cgagagccag
    10381 ccgtggctgg atgctttcgc cagtagcggt acggaagttt cccccgacac catgatggcg
    10441 gttagttgcg ctagagataa tttcaagtgc aactaatttc ctgaaaggaa gcgaggaggg
    10501 ggtaaggggg tggagtcgtt ggtaaggcct gtatcccttg gcctaggcca gaaggcaggt
    10561 acataggtta ggtaggtcac tgtataagcg cgcgatagtt tctggtgaaa tgattatcga
    10621 gctaactatt gagtgataaa ctatgtcggc agcaaccaaa tcggcactga tccgtacact
    10681 gagcactgta ccactgagaa cagaggagcg ttacagcttt ctggctgatg ttgtaacgat
    10741 ccttgagtca caggggatgc atgtggcaag caatgtcaca gtaagaatcg atggcagaaa
    10801 tttccgtgtt gatattctcg caacagcaaa aactggtggg agtgtggcta tcgagatcga
    10861 tcgtagctct ccgcgacccc gctcagtcat gaaattgcgt gaattggcac gtcgtggcac
    10921 tgaaggcttt gtgttgcttc gaatgccaaa gaaactgacc agttatagcg atgctgggat
    10981 cgatatcatc ccggcgaatg gcaagggggc gtcatgctga aaatcacccc aaactttgct
    11041 caggagcgcg gtttaaacca gctgcggcac cagtggaaac agcatcgcac gtacctgatg
    11101 tatgcaccca ctggctctgg caaaactggt cttgcagcat tcgtcactgc cggaatggtt
    11161 gaacgtggta tgcgggtgat gtttgtctgt ccgtacacca tcctgctgaa ccagacagcg
    11221 gaacgtttca ccgaatacgg tttgccgtgg gaagaaatca gttttgtatg gcgtgatcac
    11281 cctaactatg acccgtcacg actgattcag attgccagtg ccgacacgct gatccgtcgt
    11341 gagtttcctg acaacatcga tctgctgatc atcgatgaag cacacatgaa gcgtcgtgcg
    11401 ttacttgagg ttatccggga caaagatatc agggttcttg ggctttccgg caccccgttc
    11461 gcagcctgga tggggaagta ctacgaatgc ctgatcaagc ctacaacgat tcgtgaactt
    11521 attcagcgtg gtgatctgag cgattatgag ttttttgccc cctcaatgcc tgatctggct
    11581 ggcgtaaaaa ccagtaacac cgtatttgga cgggattaca acgaggaaca gctcgcatcc
    11641 atcatgggga gttcggatct ggttggcgat atcgtcagca actggcttga gaatggcgag
    11701 gatttaccga cgatctgctt ctgcgtgaat gtggctcatg cgaactttgt tacccgtgag
    11761 tttttacagt ctggcattgg cgcagaggtg atgacggcag atacccccca tgatgaacga
    11821 caggacatca ttcgccgttt tgaagagggt gcgaccaaaa taattgtcaa cgttggcgta
    11881 ctggttgcgg gatttgacag cgatgttcgc tgcctgatct acgcccgtcc gaccaaatca
    11941 gaaatccgct ggttgcagtg catcgggaga gcgttacgta ctgcgtctgg taaaaaacgc
    12001 gcattgattt tcgaccattc cggcacggtt catcggctgg gcttccccga ggatatcgag
    12061 tatgacgaac ttccgggaaa aaatgacgga atgaaagcgt ctgctggcgg gagtgaggtt
    12121 aaggctgaga aacttcccag ggaatgcccc aaatgccact tcatgaagcc tgctggcgtg
    12181 catatgtgtc ccaaatgtgg atttcgtcct cttggtggtg atgacgtggc gacagatcgc
    12241 gatcgtaagc tttcacgcgt caacaaaggg aaacgcgaat acacccgtga ggaaaaacag
    12301 cgttggtgga gcgagatcaa ggggtatcag aattaccgca acgcgacagg taagccactg
    12361 agtgacggat ggtgtgctca tacctacaag gagaagttcg gggtttggcc taaaggcttc
    12421 agtaatgcgc cgctgcaaac ctcagttgaa gtgtacaact tcatcaagtc aaagaccatt
    12481 gcctatgcca aagggcgcaa gaaagccatg acaggaggcc agcatgcgaa ctagagatgc
    12541 agccgtgggg cactggagcc ggatttttga atattacggc atgcctcctg ttactggctt
    12601 gaaacattat agcggcccct gcccgatatg tggtgccaga ggtaaatttc gctgtgatga
    12661 taaggatggt tccggttcat ggatttgtgt ctgtggtcac ggggacggga tgaatctgtt
    12721 gcaacttgcc acgggtaagc cctgggtgac gttgtgtgat gaaatcgatc ggctgatcgg
    12781 aaatacctgg aagagggaga aagtcagcca gcctgtaaca gagataagca gaaagcggaa
    12841 gctggtcatg gataagtttg ccggactccc gtgtctgcgg ggcacaacag gtgaggcgta
    12901 cctgcagggg aggggaatac tccagttacc gatcgaatcc gtgcgtttct gtgaccgtca
    12961 gatcgccagc gggcgcgaat atcaggcaat ttacgccatt gcaacagatg acaaaggttc
    13021 tctttgctat ctgcatcgta cgttgctgga tggtgatcgc aaggcgaatg tagaggcggc
    13081 taaaaaaatg actgcgctac aggagttgcc tggtttgcag catgctaaat cggtggcaat
    13141 acgcctgtat ccggtgtcgt ccactctggg gatagccgag ggtatcgaaa cggcgctttc
    13201 atgccgtcaa atcttccgct gcaatgtgtg gtcaacaatg aactccggtt ttatggagaa
    13261 gttcattgcg ccacctggcg ttaatcacct gattatcttt gctgacaatg atgcgcacgg
    13321 cgcaggtctg gcggctgcct ttaaatgtgg gcataagaat ctcatgagtt gcaatgacgt
    13381 tgagaaagtc agcattcgct ggcctgactt gccggatttt aacgacatgc tcattcaggg
    13441 gtgtgaagcc cgtgaacatg tgttgacgcg caaattcaaa gcggaggctg cctgatggaa
    13501 atagagatga tcaaggcggc taatggcgta tttgtaccgg cgtatgagcg cgatttaccc
    13561 cgactggcaa aatttaaaaa cggtgagctg tatacactgg aagcaaaact tacccgtaac
    13621 ccatcttttc accggaagat gttcgctttt cttaatttct gctttcagta ctggtgcgct
    13681 gaacatgctg gatacgaatt ctctgatgaa gcgacgcagt ttgatgagtt tcgtaaaaat
    13741 ctgacaattc ttgccgggtt ctatgatgtg gtcacaacca taagaggcga ggtgagatat
    13801 cgggcaaaaa gcctgagtta cgcgaatatg gatcaggatg agtttgaacg ttgctacaac
    13861 gcaatgatta atgccgcgtt aaaacatgtg ttcgggcgct caaacaatcc tgaactgaat
    13921 aaccgcctgc tgtcgttttt ctgaggtgat gatgaagcaa cgtaaaccaa aaaaatgcaa
    13981 agtgtgcggc tcctcgtttg tgccgttccg ctcatatctg aaagtttgct gtggtcagtg
    14041 cgcactggaa ctggtcagaa aagaaaaggc gatagcttca gcaaaagagc aggcagacaa
    14101 gctgaaagcg cgcaggaggg acttacagcc ccgcagttac tggattaagc aggcacaaca
    14161 ggctgtgaat gcttatatca gggagcggga gcgtcatttg ccgtgtgttt catgcgggac
    14221 gttcgattca gcccagtggg atgcaggcca ttaccgtaca acagctgcgg cacctcagct
    14281 cagatttgat gaacgcaata tccataagca atgcgtggtg tgtaaccagt acaaaagcgg
    14341 aaatctcgtt ccgtatcgtg tcgaactgat taaccgcatc gggcaggaag cagtagacga
    14401 aatcgaatca aaccatagtc gccaccgctg gactgtcgaa gagtgcaagg cgatcaaggc
    14461 agagtatcaa cagaaactta aagacctgcg aaacagcaga agtgaggccg catgacgttc
    14521 accgtaaaaa ccattcctga catgcttgtt gaggcatatg gaaatcagac cgaagtggcc
    14581 cgaatactga actgcaatcg tgccacagtc agaaaataca ttggcgataa agaagggaaa
    14641 agacacgcta tcgtcaacgg tgttcttatg gttcaccgcg gatggggtaa agatactgat
    14701 gcgtgatatc cggcaggttc ttgagcgctg gggggcatgg gcggcaaata accatgagga
    14761 tgttacatgg tcgcccattg ctgccggatt taagaggctg atccccgaaa aagtaaaatc
    14821 acgtccacag tgttgtgacg atgacgcgat gattatatgc gggtgtatgg ctcgccttaa
    14881 cagaaacaac agcgatctgc atgacttgct ggttgattat tacgtgttgg gggagacgtt
    14941 catggcgctg gcacggaaac atgggtgctc tgacacctgt ataggtaaac gccttcacaa
    15001 agcggagggg attgttgaag gcatgctgat gatgctggga gtgaggcttg agatggatcg
    15061 gtatgttgag cgtgaattgc cgggagggag aacctctgta ttttatcagc gaaaaaatag
    15121 tttacgatcg taaaaatctg catatcatga taagagtggt tacattgcca cgctgcttaa
    15181 cccgccgatg cgcgggtttt tttgtaccca gaatcctgtg agctatacgg aaagtacaca
    15241 gaaaggaagg tgcgaccaca attaataaca aaatcttaaa aatcgcacat ggcactatta
    15301 gttttctaaa tattgtgtat tttttgtatt gcaggatgac cctgtaacga agtttgcgta
    15361 acagcatttt gctctacgag tttgccagcc tcccccagtg gctggctttt ttatgtccgt
    15421 aacatcctgt gtatcaataa atgttgttgt ctacgtacgt caagtagccg catgagatct
    15481 gaccagatat gttaaggttg cagctctctt tgaatataat tatcattttc attacgttat
    15541 tgttacgttt atccggtgcg ccgtaaaacg ccgtccttca gggggtggag gatgtcaaga
    15601 atatagttat cgtatggtgc tcaaggagta ttgtgtaata tgaaaataat tatttttaga
    15661 gtgctaactt ttttctttgt tatcttttca gttaatgtgg ttgcgaagga atttacctta
    15721 gacttctcga ctgcaaagac gtatgtagat tcgctgaatg tcattcgctc tgcaataggt
    15781 actccattac agactatttc atcaggaggt acgtctttac tgatgattga tagtggcaca
    15841 ggggataatt tgtttgcagt tgatgtcaga gggatagatc cagaggaagg gcggtttaat
    15901 aatctacggc ttattgttga acgaaataat ttatatgtga caggatttgt taacaggaca
    15961 aataatgttt ttttatcgct ttgctgattt ttcacatgtt acctttccag gtacaacagc
    16021 ggttacattg tctggtgaca gtagctatac cacgttacag cgtgttgcag ggatcagtcg
    16081 tacggggatg cagataaatc gccattcgtt gactacttct tatctggatt taatgtcgca
    16141 tagtggaacc tcactgacgc agtctgtggc aagagcgatg ttacggtttg ttactgtgac
    16201 agctgaagct ttacgttttc ggcaaataca gaggggattt cgtacaacac tggatgatct
    16261 cagtgggcgt tcttatgtaa tgactgctga agatgttgat cttacattga actggggaag
    16321 gttgagtagt gtcctgcctg actatcatgg acaagactct gttcgtgtag gaagaatttc
    16381 ttttggaagc attaatgcaa ttctgggaag cgtggcatta atactgaatt gtcatcatca
    16441 tgcatcgcga gttgccagaa tggcatctga tgagtttcct tctatgtgtc cggcagatgg
    16501 aagagtccgt gggattacgc acaataaaat attgtgggat tcatccactc tgggggcaat
    16561 tctgatgcgc agaactatta gcagttgagg gggtaaaatg aaaaaaacat tattaatagc
    16621 tgcatcgctt tcattttttt cagcaagtgc gctggcgacg cctgattgtg taactggaaa
    16681 ggtggagtat acaaaatata atgatgacga tacctttaca gttaaagtgg gtgataaaga
    16741 attatttacc aacagatgga atcttcagtc tcttcttctc agtgcgcaaa ttacggggat
    16801 gactgtaacc attaaaacta atgcctgtca taatggaggg ggattcagcg aagttatttt
    16861 tcgttgactc agaatagctc agtgaaaata gcaggcggag attcataaat gttaaataca
    16921 tctcaattca gtcagttgtt gccggtctga taatagatgt gttagaaaat ttctgcatgg
    16981 tgaatccccc tgtgcggagg ggcgactggt gaacggtatg atctctttga tgatcgtaag
    17041 cgagaatacg cgggtttggt ggcaccagac cgaactcacc gggaggcacc cggcaccatg
    17101 cagtattcag agattaggca tatatccagg ctcctcatcg caggagcctt tttacatgca
    17161 aaaaaaaacc gctcctggga agagcggctg gcaagaagaa acaatatgaa caattaatta
    17221 acgatgggaa taatacctta gagtaatcac cttgcgcaac tgtaagggca tatttcatct
    17281 ttgcgggctg tttttctgtg tggcttctgt gtttccggag ggcagcctgt accttttctg
    17341 actcagaaca ttatcccggc cgggaggatt catggcattt aaacactacg atgtggtcag
    17401 ggcggcatcg ccgtcagacc ttgctgaacg aataactcaa aaactgaagg aagggtggca
    17461 gccttatggt agtgcgctga tttcgacagc tggttatggt gcagagttta tccagccagt
    17521 tgtgagtgag ggggagcttc cctcacttgc agagtcaggc aatcacccgc atgtttcagc
    17581 gaagcctgaa gcagcgccgg aatattacta tgtgattgcg cttgccggtc agtccaatag
    17641 catgtcatac ggtgaaggtc ttccgttgcc ggagacatat gaccgcccgg acccgcgtat
    17701 taagcagctg gcgcgccgca gtacggtgac accgggcggt gcagcatgca aatataacga
    17761 catcattccg gcggaccatt gtctgcatga tgtgcaggac atgagccgtc ttaaccatcc
    17821 gaaagcggaa ctgtcaaagg ggcagtacgg aaccgtgggg caggggctgc atattgccaa
    17881 aaaattgctg ccgtttatac cggcgaatgc gggcattctg ctggttccgt gctgtcgtgg
    17941 tggttccgcg ttcaccaccg gagccgatgg cacatacagt gacgctggcg gtgcctcgga
    18001 gaattcaacc cgctggggtg tggacaagcc gctgtataag gaccttatcg gtcgaacaaa
    18061 agcagcactg aagaagaacc cgaaaaatgt gctgtttgcc gtggtgtgga tgcaggggga
    18121 atttgatttt ggcggtacgc cggtaaatca cgcagcacag tttggtgcgc tggttgataa
    18181 attccgtgca gacctggcag atatggcagg tcagtgcgtt ggtggctctg ctggcggtgt
    18241 tccctggata tgcggggaca cgacgtattt ctggaagcag aagaacgaat ccacgtacca
    18301 gacggtgtac ggcagctata aaaacaaaac ggaaaagaat atccatttcg taccgttcat
    18361 gacggatgag aacggggtga atgtgccgac gaacaaaccg gaagaagacc cggacattcc
    18421 gggtatcgga tattacggtt cgaaatggcg tgacagctcc gccacctgga cgtcacagga
    18481 cagggcgagc catttcagtt catgggcacg ccgcgggatt atttccgacc gtctggcaac
    18541 ggcgattctg cgccatgcgg gaagggtggc gctaaacgtg ggggcaccat cgacagtatc
    18601 agaggtgcgt ccgtcatcgc cttccggtgc agaagtcaca ggcgtcacaa cactgctctc
    18661 ttaccttgcc agcgagtcag agggaagcct gaaagtacag ggatggtcag ccagtggcgg
    18721 cagggcagaa gtggtcagcg atgcggaggg aaccggaggt aaggctgtga aggtgaccaa
    18781 ggaagccggt aaaagcagct gggtactgga gtacgctgcg ggcaacggcg cggctctgtt
    18841 acagaaaggg ggacagattc gctgccgctt taaggcttcg ggagcgctgg cggcgaacca
    18901 gtatgttatg gcgttttact ggccggtatc gtcactgcca cagggcgttg tcctgaccgg
    18961 cgatgggggg aataacctgc tggcagcgtt ctacatccag acggatgcaa aagacctgaa
    19021 tgtgatgtac cacaatgcga aagtggcgac aaacaacctg aaactgggaa gctttggcgc
    19081 atttgataac gaatggcata cgctggcttt ccgctttgcc gggaataaca gccttcaggt
    19141 gacgccggtt attgatggtc aggatggcac accgttcacg ctgacgcagt caccggtcag
    19201 tgcattttcg gcggataagt tgcatgtgac agacattacc aaaagtgcga cttacccggt
    19261 actgatagac agcattgcgg tggaagtgaa taacgcggac gccgctgcat gataaaaaaa
    19321 gccgccagtt accggaatgg actggcggtg gtaataccta tggagaaaaa ataaaggaac
    19381 gatactttcg tactctgttt ttttaaatga aaacagttct tattgtcaac aataacggaa
    19441 agaaattatg acatttctga accagttaat gctgtacttc tgtacggtgg tctgtgtgct
    19501 gtatctcctt tcgggtggat accgggccat gcatgacttc tggcgcagac agattgacaa
    19561 aagggccgct gagaaaatca gcgccagtca gtcagccgga agcaaacccg aagagccgct
    19621 cgtttagcgg caactttctt aatcacacct ttcgacgaga aaatcccatg tcagaaatta
    19681 catccctggt cactgctgaa gcagtgaagg acgtcctgcg ctctgaagaa gtcctgagcg
    19741 cactgaaaca gaaacttcgc cataacctgg aagcgcgtct tgatgcagaa gtggatgcca
    19801 ttctggatga actgctgggc gcaccggcag ctccggagcc ggaaggcatc gcgggagagg
    19861 ggagtgcttc agatagcggt gaccccacac ctgacagcga catgatgatg taagcatgcg
    19921 tcagggacca tcggtgtgtg ccggtggtct tttttattgt tgtgagcttc cggattgcgg
    19981 gaggcggggt atgtaccaga tggaaaaaat cacaacaggt gtgtcataca ccacgtcagc
    20041 ggtgggaacg ggctactggt tcctgcagtt gctggacagg gtttccccgt ctcagtgggc
    20101 ggcaataggc gtgctgggga gtctgctgtt tggtctgctg acgtacctga cgaacctgta
    20161 tttcaaaatc agagaggacc ggcgtaaggc ggcgcgggga gagtaagctg atgagccgga
    20221 aattccgcta tggtttatca gctgtcgttc tggcgctgat tgccgcaggg gcttctgcgc
    20281 ctgaaatact ggatcagttt ctggatgaaa aagaaggcaa ccacactacg gcataccgtg
    20341 atggtgcggg catctggacc atctgccgtg gagccacccg ggtggatggc aagcctgtta
    20401 ttcctggcat gaagctgtcg aaggggaaat gcgaccaggt taacgcaatt gagcgtgata
    20461 aggcgctggc atgggtggcg aaaaacatca aagtgccact gactgaaccc cagaaagcgg
    20521 gtattgcgtc attctgtcct tacaacattg gcccgggtaa gtgtttcccg tcgacgtttt
    20581 ataaacgaat taatgcagga gatcgaaaag gtgcctgcga agcgattcgc tggtggatta
    20641 aggacggcgg cagggactgt cggaatcgct caaataactg ttacgggcag gtatcgcggc
    20701 gtgaccagga gagcgcgctg gcgtgctgga gtatcgacag ataagcagaa tgttctgctg
    20761 aaaaatgccg ttgaccaacg cggacggata acacgaaatc ctgcgaactg gcaaaattta
    20821 agtgagtaaa agtaaaaacc ccgtttgttg gcagcaagcg gggttttgtg tttctgacct
    20881 tgaataaggc aagggagaaa ttatgggtag ggaggttctt tccctgtgag gaagtataaa
    20941 agattctttc tgaggttgtc cattatgaaa ggcattgagg tggagacgcc agcaagtctg
    21001 gatttaacaa gagcggcagc ttttgccatt cgtattgtgg caattgctgt tctggtctgg
    21061 gcaatccgtt ggtggtgata tgaatcgtgt gctgtatggc gtgcttattg tcctgctgct
    21121 tggttgtgga tcgctgtggc tggcaacaga ccattaccgt gataacgcca tcacctacaa
    21181 agagcagcga gatgatgcga agacaaaact cagccaggcg aacgccacca ttgctgacat
    21241 acagcagcgc cagcgtgatg ttgctgcact cgatgccaga tacaccaggg agcttgctga
    21301 tgctaacgcg actattgaaa gtctccgtgc tgatgtttct gctgggcgta agcgcctgca
    21361 aatcgccgcc acctgtgcaa agtcaacgac cggagccagc ggcatgggcg atggagaaag
    21421 ctcaggactt acagcagatg ctgaactcaa ttattaccgt ctccgaagtg gaatcgacaa
    21481 gataaccgcg caggttaact acctgcagga gtacatcagg atgcaatgcc tcgattaaca
    21541 gagccagctc aatcgctggc cttttttatg aggagagata taacctgaat caatgccagg
    21601 gcattatcaa caaatggatc actgggtaag ctcgtttggt tttaaatgtc cgtcgagaat
    21661 cagattgtga ttttcccgat gcagaaccag tgtgcccata taatcaggtt taaatctcca
    21721 tccattcatt ttttttgcaa ttctggacag acagtgatta tgtgtgaaaa tcacagtaac
    21781 gtgatctgat tttgatattt taaggatatt atttactgcg ttatcatcac atgtcgatat
    21841 gtccggaata gttgctgtct tacctcccga aaaaaaagtg gctgtctgca ttgttcggag
    21901 tgtgtctgta gaatagagac tgtagtatgg aaacattctt ctgaatttat ctccatattg
    21961 ttgagctttg ttagctccgt ttactgtgat tccctcatgt gctgaaagac atattctttg
    22021 ggagcgatcg catctttccc catgccggat gagaaaaata atgggatgtt tctggttaat
    22081 ctcagtgaca tcattcacat caagtctgac tggagttctt gcaatgtata tgatggatac
    22141 agaaacgatt atgacaagta aaaatataag aagagatttt gttagcgttt gatttaacat
    22201 ataaattccc ttgtctgtgt tgaaaatggg cattattatg cgtaaatctt atgtaatcct
    22261 taaggattgt ctgacgtggt gatatgacgc gaaatacagt gggtacacag aatgctgtga
    22321 acaacgacga aatgggcact ggtagcggta ctggtgcttt ttttattggt tggttgctca
    22381 agtcttgata aagcgcgtta gctttttggt attgcctctt aggtctgtaa aattgccgac
    22441 ggtgtccggc agtgcatgca gggctgatcg tctatgagaa cagaatatat tgctaaaaat
    22501 gaagtgtacg tcaatgctta taaagcatgc aattctgtat ttctaactat tcaataaaat
    22561 aaattctttc tgtcgcagtg aatatacaaa tgttgatcag cgctcgatgt ggcgacgggt
    22621 ttctatatca ggagacttta gcatttatcc gcgaggcagc ctatctggtg ctgtagtgga
    22681 atgaagcgga tataacctaa ataaggttaa acattaatca gacatggctc tgctgtgtga
    22741 aatctgaaaa tttacagcag tcattgtgca tcagttttta acagagaacg tcagaaggtg
    22801 acatggcaaa gctggactgg aaaaagctgg agcaggcatt tcgacgcgaa catgtcaaaa
    22861 ccggcataaa gcttcaggac tggtgccgtc agaacaatat cagttatgac acggctcggc
    22921 ggtacattaa actgcgcaaa cactccccca gaaatacaga gaaaagtgcg cagaaaaatg
    22981 cgcaaattga tgatcaaaaa agtgcgcaga aatctgacgg caactccagt catgacgagc
    23041 cttcaggcga tgatggttgt gatgaaaaat gcgcaaattt ggcagaaacg aaacggactc
    23101 gtggttccag acttttgcca ccttcaaatg ctttttctca gcgtaacacc cacgccgtca
    23161 gacaccgtgg atacgcgaag tatctcgagg cagataacct catagatgat gcagtcggaa
    23221 tggaacttgc cgatgaactg gtgtttaccc gtgcccgcgc actttcagta acaggaacac
    23281 tgaaaaagat gttcgccgac ctgaaagagg cggctgacgt ggaaacacgg gtagctctgt
    23341 acgacaaaat acttaaagcc gaacaggctc ttgaccggaa tattgcccgt atcgagtcaa
    23401 ttgaacgctc attgctgacg ctggacgtcc tggctga
//
LOCUS       DAZGJT010000076        22933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZGJT010000076 DAZGJT010000000
VERSION     DAZGJT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.076"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..114)
                     /locus_tag="ACHKFK_003245"
     CDS             complement(<1..114)
                     /locus_tag="ACHKFK_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein"
                     /protein_id="HFO9053518.1"
                     /translation="MSRHATDWAWETDPGSSSLKLILLSMADRADEYNLCYP"
     gene            complement(130..330)
                     /locus_tag="ACHKFK_003246"
     CDS             complement(130..330)
                     /locus_tag="ACHKFK_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053519.1"
                     /translation="MEIITRIDAAKRGLKRYYTGKTCKHGHDSERWVYNGHCVECTME
                     SNRRIRAEIKQIMINSSPQHSS"
     gene            complement(481..711)
                     /locus_tag="ACHKFK_003247"
     CDS             complement(481..711)
                     /locus_tag="ACHKFK_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053520.1"
                     /translation="MSDNGHFDFKKHWLALTPDEREAFAQEAGTTSHYIQTHLTGKRK
                     MPGKVLMNGLFKACKTRQWLRSKAELAYFFYS"
     gene            866..1450
                     /locus_tag="ACHKFK_003248"
     CDS             866..1450
                     /locus_tag="ACHKFK_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9053521.1"
                     /translation="MKSIQDVRRQNLNDLIDREFNGVQTRMAEKLGTQANLVNRWALG
                     KKVIGDQVARKIEAAANKPRNWLDIDRSLSQEGFQPVGPSDIGQLAAHNLERWMSESR
                     DLSTQGKLHRASGVAQVTISRLLNNEVSVSISTLENVASAFGRHGYELLIHPHDPATI
                     NYDRSRYALLPETEKAKIESYIEFVINQNEKNKQ"
     gene            1759..2058
                     /locus_tag="ACHKFK_003249"
     CDS             1759..2058
                     /locus_tag="ACHKFK_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053522.1"
                     /translation="MNVNQQKNLQKIMLAFDKDYRLSEQLYDRQVELIESIRLHQLAS
                     TFDVVTGKGVRQEVLEAAKDSPEFEELMDAYRREAMAIIARWDLADQLDGQRDAA"
     gene            2055..2876
                     /locus_tag="ACHKFK_003250"
     CDS             2055..2876
                     /locus_tag="ACHKFK_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease-like domain-containing
                     protein"
                     /protein_id="HFO9053523.1"
                     /translation="MKPGIYFDISNEDYHAGDGVSKSQLDMVAKNPALLKWVQAAPED
                     EEKKSALDMGTALHCLLLEPGEFDKRFIVSPKFDRRTKQGKADEEAFLRDVADMGITV
                     LDVEQWRKLELMRDSAMAHPAARWMLEAPGYCEASMYWNDEETGELCRIRPDKWLNEH
                     NVIVDVKKVADMDRFARHIEEFRYHVQDAMYREGAMRVTGQPHGFFFLAVSESIDCGR
                     YPVRVFELDAPDVDAGHALFRRDLNTYHECRINDEWGGVEIIKRPDWARKQDMYV"
     gene            2873..3754
                     /locus_tag="ACHKFK_003251"
     CDS             2873..3754
                     /locus_tag="ACHKFK_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecT family recombinase"
                     /protein_id="HFO9053524.1"
                     /translation="MSNDIAITSQPGATVGTAAAIFSPEGMNQLVRFAELMSQSKATV
                     PKHLEGKPADCLAVTMQAAQWGMNPFAVAQKTHVVNGTLGYEAQLVNAVVSSSSLLAT
                     RLNYRWSGDWSNVNGKTDKSPNLTVTVSAVLKGEAEPRELTISMAQAGVRNSPLWEQD
                     PRQQLAYLCTKRWARLHAPDVLLGVYTPDELQETAPRVERDITPQTTTAAGMNSLINA
                     KTVKKPDEQTRKADSRDPEEMLMAFTSAPMNYSTVSELDKAYKYIAQKLSDDDELLAK
                     ATDVYSVRREELNETSM"
     gene            3804..4052
                     /locus_tag="ACHKFK_003252"
     CDS             3804..4052
                     /locus_tag="ACHKFK_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9053525.1"
                     /translation="MKGALGKKELLAVVPLSWSTIDRMERAGEFPKRWYITDKRCAWN
                     RDEVERWLDERQAASPAEFQGKKPPVQQRVYRPVSNAA"
     gene            4210..4461
                     /locus_tag="ACHKFK_003253"
     CDS             4210..4461
                     /locus_tag="ACHKFK_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO9053526.1"
                     /translation="MRSPSKIVAVTPAAIEIEKRAIEREKKGQFRIAAHLWLQCMDVA
                     SGDVERARIAVRRDQCITKGNGLRRGDYSGIGCCGVVYD"
     gene            4454..5104
                     /locus_tag="ACHKFK_003254"
     CDS             4454..5104
                     /locus_tag="ACHKFK_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO9053527.1"
                     /translation="MTKKYTLIYADPPWVYRDKAADGNRGAGFKYPVMSVLDICRLPV
                     WDLADENCLLAMWWVPTQPLEALKVVEAWGFRLMTMKGFTWIKCGSRQPDKLVMGMGH
                     MTRANSEDCLFAVKGKLPTRINAGIVQSFTAPRLEHSRKPDIVREKLVQLLGDVSRIE
                     LFARQSSHGFDVWGNQCEDPAVQLHPGYALDIGGLTNAFSNAPLSPTDNQGRERAA"
     gene            5101..5295
                     /locus_tag="ACHKFK_003255"
     CDS             5101..5295
                     /locus_tag="ACHKFK_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9053528.1"
                     /translation="MNRASPADLRKCLETANMLAHSGIRFVPIPAVTDAEFATLSAIF
                     ENKIESLAAEAKMEENQQNY"
     gene            complement(5299..6549)
                     /locus_tag="ACHKFK_003256"
     CDS             complement(5299..6549)
                     /locus_tag="ACHKFK_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9053529.1"
                     /translation="MLTDTKLKNLKPQDKLYKVSDRDGLYVAVLTSGTVSFRYDYRIN
                     GRRETLVIGQYGRDGISLAEAREELIAAKKLLKAGQSPAAAKRDGIKKIRGAETFAVH
                     TDSYMKHVILADSTRAMKQAVIDRDILPVLGNKMMAEITTSMVRDLCDRIVERGGRAT
                     AVQAREIISSVYRHANDRGHGLFNPAADIKPSSIAIFKPRERTLTPEEIGLFFRTLDA
                     IGAMGTMKMALKLVLITMVRKGEFTNATWDEIDFKKWTWTIPSDRMKGSRAHVIYLPK
                     QAQDILVGLQMCAGGSEYLVPGRYNFRKPLSNAALNSLIDRTVKIINEDGEHIQGFTV
                     HDMRRTASTLLHEAGYPSDWIEKALAHEQKGVRAVYNKAEYARQRAYMLQQWADMIDS
                     WINGEHTDLIPFSPSKFEKWMAGE"
     gene            complement(6742..8319)
                     /gene="guaA"
                     /locus_tag="ACHKFK_003257"
     CDS             complement(6742..8319)
                     /gene="guaA"
                     /locus_tag="ACHKFK_003257"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO9053530.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(8388..9854)
                     /gene="guaB"
                     /locus_tag="ACHKFK_003258"
     CDS             complement(8388..9854)
                     /gene="guaB"
                     /locus_tag="ACHKFK_003258"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO9053531.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            10016..11392
                     /gene="xseA"
                     /locus_tag="ACHKFK_003259"
     CDS             10016..11392
                     /gene="xseA"
                     /locus_tag="ACHKFK_003259"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO9053532.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQTEGLFDLQYKKSLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANKRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPIVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     THQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTRQLVQQNPQSRIHRAQTRIQQLEYRLAETLRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTSAADGAVLKQVKQVKVGETLITRLGDGVVISEVSAVTKTRK
                     SRKKTSNP"
     gene            complement(11461..19512)
                     /locus_tag="ACHKFK_003260"
                     /pseudo
     CDS             complement(11461..19512)
                     /locus_tag="ACHKFK_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF823 domain-containing adhesin"
     gene            complement(19633..20610)
                     /locus_tag="ACHKFK_003261"
     CDS             complement(19633..20610)
                     /locus_tag="ACHKFK_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family autotransporter-associated protein"
                     /protein_id="HFO9053533.1"
                     /translation="MKQDKRRGLTRIALALALAGYCVAPVALAEDSAWVDSGETNIFQ
                     GTIPWLYSEGGSATTDADRVTLTSDLKGARPQGSETDKRLYSGDKLTVSWEIGDTEGD
                     VDLGGLGDNAKTIDTIRWMSYKDAQGGDPKELATKVTSYTLTDADRGRYIGIEITPTT
                     QTGTPNVGTALHLYDVSTVSGGGSDSDNVAPGPVVNQNLKVAIFVDGTSINLINGSTP
                     IELGKTYVAKLYSDENKNGKFDAGTDADVTANYDFRWVLSGSSQQLGTSGGIVNSSFD
                     NNNLVIPATNDEARTNLNGPARDGKEALSIPTNGDGVQGYKLHIIYKHK"
     gene            complement(20657..22834)
                     /gene="sinH"
                     /locus_tag="ACHKFK_003262"
     CDS             complement(20657..22834)
                     /gene="sinH"
                     /locus_tag="ACHKFK_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like inverse autotransporter SinH"
                     /protein_id="HFO9053534.1"
                     /translation="MLRWKRCIILTFISGAAFAAPEINVKQNESLPDLGSQAAQQDEQ
                     TNKGKSLKERGADYVINSATQGFENLTPEALESQARSYLQSQITSTAQSYIEDTLSPY
                     GKVRSNLSIGQGGDLDGSSIDYFVPWYDNQTTVYFSQFSAQRKEDRTIGNIGLGVRYN
                     FDKYLLGGNIFYDYDFTRGHRRLGLGAEAWTDYLKFSGNYYHPLSDWKDSEDFDFYEE
                     RPARGWDIRAEAWLPAYPQLGGKIVFEQYYGNEVALFGTDSLEKDPFAVTLGVKYQPV
                     PLIVVGTDFKAGTGDNTDLSVNATLNYQFGVPLKDQLDPDKVSAAHSLMGSRHDFVER
                     NNFIVLEYKEKDPLYVTLWLKADVTNEHPECVIKDTPEEAVGLEKCKWTINALINHHY
                     KIVAASWQAKNNAARTLVMPVIKENTLTEGNNNHWNLVLPAWQYSSDQAEQEKLNTWR
                     VRLALEDEKGNRQNSGVVEITVQQDRKIELIVNNIANVPEENNHSHEASAQADGVDGV
                     VMDLDVTDSFGDNTDRNGDALPEDNLTPQLYDAQDKRVTLTNKPCSTDNPCVFIAKQD
                     KEKGTVTLSSTLPGTYRWKAKAAPYDDSNYVDVTFLGAEIGGLNAFIYRVGAAKPSNL
                     IGKDKEPLPLNNIYRFVLWRDNNKDGVFQQVEKLTDEEMAQYDYKWEFTGKSINGEVG
                     AQANTSNEDIVIPATNREAAQTYGAQAGDGLQGYGLRVLYTKK"
BASE COUNT         5536 a         5918 c         5720 g         5759 t
ORIGIN      
        1 ggggtagcag aggttatatt catcggctct gtcagccatc gagagcagta tgagctttaa
       61 tgacgagcta cctggatctg tctcccaggc ccaatctgtt gcatgtctgc tcatgattaa
      121 tctccgctat cagcttgaat gttgtgggga ggaattaatc atgatctgct taatctctgc
      181 cctgatacga cggtttgatt ccatggtgca ctcaacacag tgtccgttgt aaacccagcg
      241 ttcactgtca tgtccgtgct tacatgtttt tccggtgtag tagcgtttaa gtccgcgctt
      301 tgcggcatca atacgtgtaa tgatttccat ggtaagccct gttattagta ttgggattac
      361 ggttattttg tgctgacaca aaaaaaagat caaccatatt tggtttttta ttacctttaa
      421 ggtgcgaata gatatgaaaa gaccgccgga tggcggtcta cagagggttg tggctggata
      481 tcatgagtag aagaagtatg ccagttctgc ttttgagcgc agccattgtc ttgttttaca
      541 ggctttaaaa agcccattca tcaatacctt acctggcatt ttgcgcttac ctgttaagtg
      601 agtctggata tagtgactcg tcgttccggc ttcctgtgcg aaggcttcac gctcatccgg
      661 agtaagtgca agccagtgct ttttgaaatc gaaatgtccg ttatcgctca tagctattgc
      721 ctgatattta tttcagataa taaatattca cccataaggt aacaaaaatc aaggatagtt
      781 acctatgggg tgcatttacc cgttgggtaa tattgcttta aattgaatca tctactgatt
      841 catatatgag gcgattttcc agaaaatgaa aagtatccag gacgtccgca ggcaaaatct
      901 caacgacttg atcgaccgtg aattcaatgg tgttcagacg cggatggcag aaaaacttgg
      961 aactcaggca aatctggtaa accgctgggc tcttggcaag aaggttatcg gcgaccaggt
     1021 tgcgcgaaaa attgaagctg ccgccaataa accccgtaac tggcttgata tcgatcgctc
     1081 gctttctcag gaaggttttc agcctgtcgg cccaagcgac attggacagc tggcggctca
     1141 caacctggaa cgctggatga gcgaaagccg cgacctttca acgcagggaa aactacaccg
     1201 cgcatccggc gtcgcccagg tgacaatcag ccgcctgtta aacaatgagg tcagcgtttc
     1261 catttccacc ctggagaatg ttgcatccgc attcgggcgt cacggctatg aattactgat
     1321 tcacccgcac gaccctgcga ccatcaacta tgatcgctcg cgctacgcat tgttacctga
     1381 aacagagaag gcaaagatcg aaagttacat tgaatttgtc atcaaccaga acgaaaaaaa
     1441 caaacaataa aatcatattt ttcagtaagt aagccgcctt ctggcggctt ttttattgcc
     1501 tgttcgatta cctaacgggt aattttttta actcatatct attgacatca aaccaaatac
     1561 gcataattat tacctcaacg gtaacaaacc gaggtaacaa gttatgcagt ggaaaatcat
     1621 caacggttgg tactgcgtta ctgcatgcgg attcatgagc tggaagttcc gcaccttaca
     1681 ggaaggcatt aagtgggctt tcgtcagcaa agaagctcgc gatgtggcca acgataacga
     1741 gatatgggag ggttgataat gaacgttaat cagcagaaaa atcttcaaaa aatcatgctg
     1801 gcattcgaca aggactaccg cctgtcagaa cagctatatg accgacaagt tgaactgatt
     1861 gagagtatcc ggcttcatca actggcctca actttcgacg ttgtaacagg caaaggcgtt
     1921 cgtcaggaag tactggaggc tgctaaagac agccctgagt tcgaagaact gatggatgcc
     1981 taccggcgag aggcaatggc aattatcgcc cgctgggatc tggctgatca gcttgatggg
     2041 cagagggacg cggcatgaaa ccgggaattt atttcgacat cagcaacgaa gactaccacg
     2101 ccggtgacgg cgtgagtaag tcgcaactgg acatggttgc caagaatccg gcgcttctta
     2161 aatgggttca ggcagcacca gaagacgaag agaaaaagtc tgcactggat atgggaaccg
     2221 cattgcactg tctgcttctg gaacctggag aattcgacaa gcgcttcatc gtttcaccga
     2281 aattcgatcg tcggacaaaa caaggtaaag ctgacgaaga agcatttctt cgtgatgtgg
     2341 cggatatggg tattacggta cttgatgtcg agcagtggcg aaaactggag ctgatgcgtg
     2401 atagcgcaat ggctcacccg gctgcacgct ggatgttgga agcacctggt tactgcgaag
     2461 cgtcaatgta ctggaacgat gaagagactg gtgagttgtg ccgaattcgt ccagacaaat
     2521 ggctgaacga gcacaacgtg atcgtcgacg tgaaaaaggt tgcagatatg gaccgttttg
     2581 cacgtcacat cgaggaattc cgctaccacg tgcaggacgc aatgtaccgc gaaggcgcaa
     2641 tgagggttac tggtcagccg catggttttt tctttcttgc cgtgagcgaa agcattgatt
     2701 gtggtcggta tccggtacgc gtgttcgagc tggatgcgcc ggatgtcgat gccgggcacg
     2761 ctctgttccg ccgggatctg aatacctatc acgaatgccg catcaacgat gaatggggcg
     2821 gagtggaaat tattaaacgc cctgactggg cacgtaaaca ggatatgtat gtatgagcaa
     2881 tgatatcgca atcacatcac aaccaggcgc aactgtaggc actgctgcgg caatcttcag
     2941 ccccgagggc atgaatcaac tggtgcgttt cgcggagttg atgtcacaaa gcaaagcaac
     3001 tgtaccgaaa catcttgaag gcaaacctgc cgattgcctg gcggtgacca tgcaggcggc
     3061 acagtgggga atgaacccgt tcgccgtggc gcagaaaacg catgtggtaa acggaacgtt
     3121 aggctacgaa gcacagttgg taaacgcggt cgtatcctct tccagcctgc tggcgacacg
     3181 cctgaattat cgctggagcg gtgactggtc gaatgttaac ggcaaaacag ataaatcacc
     3241 taatctgacg gtaactgtgt cagcagttct taaaggagaa gcagaaccac gtgagcttac
     3301 catcagtatg gcgcaagccg gagtgcgtaa ctctccattg tgggaacagg atccgcgcca
     3361 gcagcttgcc tatctttgca cgaaacgatg ggctcgcctg cacgctcctg atgtacttct
     3421 cggtgtttac acaccagacg aattacagga aacggcaccg cgcgttgagc gagacattac
     3481 tccgcaaacg accactgctg cgggaatgaa cagtctgatc aacgctaaaa cagtgaaaaa
     3541 gcctgatgag caaacgcgta aagcggatag ccgtgatcca gaagaaatgc tgatggcctt
     3601 taccagcgca ccgatgaatt acagcactgt ctccgaacta gataaggctt acaaatacat
     3661 tgcacaaaaa ctttcagatg atgacgaact gctggcaaaa gccaccgacg tttacagcgt
     3721 tcgccgggaa gaattaaacg aaacatctat gtaaccacca ccgcggcgcc acgcgcgccg
     3781 cactgcaacc aagagaggta tttatgaaag gtgcattagg taagaaggaa ctcctggcgg
     3841 tggtgccact gtcatggagc actatcgacc gtatggagcg cgcaggtgaa tttcctaaac
     3901 gctggtatat cactgacaaa cgctgcgcat ggaaccgtga cgaagttgag cgttggcttg
     3961 atgaacgtca ggcagcaagc ccggcagagt tccagggtaa aaagcctccg gttcagcaac
     4021 gtgtatatcg tcctgtgagc aacgctgcat gagtgtgctg ctaaggcact ggagcaaatg
     4081 gtcaggatgg tacttattcc tggcctctgt ttcagcatgg ctttatctgc tggcattaat
     4141 tttcagagag ggttggatta agtgagaaag ttaagccgac ttgaaaaata tcacatgaac
     4201 aaggtttcaa tgcgcagtcc gtcaaagatt gtcgccgtta ctcctgcggc gatagagatc
     4261 gaaaaacgcg cgattgaaag agagaaaaaa gggcagttcc gcattgccgc tcacctttgg
     4321 cttcagtgta tggatgttgc ttctggtgat gttgagcgtg caaggatcgc ggttcgcagg
     4381 gaccaatgta tcacaaaagg taacggcctt cgccgtggcg actatagcgg cataggatgt
     4441 tgtggggtgg tttatgacta agaaatacac actaatctat gcagatcccc cctgggtata
     4501 ccgggacaaa gccgcagatg gtaatcgcgg tgccggtttt aaatatccgg ttatgagtgt
     4561 gctggatatc tgccgccttc ctgtgtggga tttggccgat gaaaactgtc tgttggccat
     4621 gtggtgggtg ccaacacaac cactagaagc actaaaagtt gttgaagcct ggggattccg
     4681 tctgatgacc atgaagggat tcacgtggat aaaatgtggt agtcgacaac cagataaact
     4741 ggttatgggt atgggacaca tgactcgcgc caatagtgaa gattgcctgt ttgcggtaaa
     4801 gggaaaacta cctacgcgca ttaatgcagg gatcgttcag tcatttaccg caccgcggct
     4861 tgagcattca agaaagccag atatcgttcg tgaaaaactt gtgcaattgt taggcgatgt
     4921 ttctcgcatt gaactgttcg cccgccagtc gtctcatggc ttcgatgttt ggggtaatca
     4981 gtgcgaagac ccggcagtgc aactacaccc tggatacgcg ttggatattg gcggattaac
     5041 aaatgcattc agcaatgctc cgctgtcacc aacagacaac caggggcggg agcgtgcagc
     5101 atgaaccggg catcaccagc agatttaagg aaatgccttg aaactgcaaa catgcttgca
     5161 cacagcggga tcaggtttgt tcccattccc gctgtcactg atgctgaatt tgcaacgctg
     5221 tcagcaatat tcgaaaacaa aattgaatca ctggcagcag aagcaaagat ggaagaaaat
     5281 cagcagaact attaaacgtt attcccccgc catccacttc tcaaacttcg acggggagaa
     5341 cggaatcaga tccgtatgct ccccgttaat ccaggaatca atcatatcgg cccactgctg
     5401 caacatgtag gcgcgctgtc tggcgtattc cgctttgtta tatacggcgc gcacaccttt
     5461 ctgctcatgt gccagagcct tttcaatcca gtctgaagga taaccagcct catgcaacaa
     5521 cgtactggct gtacggcgca tatcgtgtac ggtgaagccc tgaatatgct caccatcttc
     5581 atttattatt ttcaccgttc tgtcgatcag agagttcagc gcggcattag ataatggctt
     5641 ccggaaattg taacgaccag gaaccagata ttcacttcca ccagcgcaca tctgcaaccc
     5701 aaccaatata tcctgtgcct gtttaggcag gtaaataacg tgcgcccggc ttcccttcat
     5761 gcggtctgaa ggaattgtcc atgtccattt tttaaaatct atttcatccc acgttgcatt
     5821 ggtgaattcg cctttacgaa ccatagtgat aagcaccagc tttaaagcca ttttcatagt
     5881 gcccatagca ccaatggcat ccaacgtgcg gaagaacagg ccaatttctt ctggtgtcag
     5941 tgttcgctct cgtggtttaa atatggcgat agacgaaggt ttaatgtcag ccgcaggatt
     6001 aaacaaacca tgaccacggt cattggcgtg acggtatacg ctgctgatga tttccctggc
     6061 ctgtactgct gttgcccgac caccgcgttc gacaatccgg tcacacaaat cacgaaccat
     6121 cgatgtggta atttcagcca tcattttgtt gccaagaacc ggaagtatgt cacggtcgat
     6181 caccgcctgc ttcattgcgc gggtactgtc agccaggatg acgtgtttca tataactgtc
     6241 ggtatgtacc gcaaacgtct cggcaccacg aatctttttg ataccgtcac gtttagccgc
     6301 agccggcgac tggcctgctt taagcagctt ctttgcagca atcagttctt ctcgcgcttc
     6361 tgccaggctg ataccgtcac gcccatactg cccgattacc agtgtttcgc ggcgaccgtt
     6421 gatacggtag tcatagcgaa acgagaccgt gcctgacgta agcacagcta catacagccc
     6481 gtcacgatcg gagaccttgt acagtttgtc ctgcggcttg aggtttttta attttgtatc
     6541 ggtaagcaca attcacccgt atagaaacca ttttcatgac ggtatgagag tataccttta
     6601 aggtaatacc gtcacctgta ccgccgaaaa atatggtgta gagtgaatag aaatgaatac
     6661 ataaaaacaa aaaccctctg caaaaacaga gggttgagtt ggcatctgaa taggaatgat
     6721 ttgctatgag ttagctgtta atcattccca ctcaatggta gctggcggct tgccgctgat
     6781 gtcatacacc acgcgggaaa taccgttcac ttcattgata atacggttgg aaacgcggcc
     6841 gaggaagtcg tacggcagat gtgcccagtg tgcggtcata aagtcgatgg tttcgacagc
     6901 acgcagagag acaacccagt catacttacg accatcgccc attacgccaa cggaacgtac
     6961 tggcaggaag acggtgaacg cctggctgac tttgtcgtac aggtccgctt tacgcagttc
     7021 ttcaatgaag atggcgtcag cacggcgcag caggtcacaa tactctttct tcacttctcc
     7081 cagaacacga acgccaaggc ctggtcccgg gaacgggtga cggtacaaca tgtcatacgg
     7141 taggcccagc tccagaccaa tcttacgcac ttcgtctttg aacagctctt tcagcggttc
     7201 aaccaggccc atcttcatct ctttcggcag gccacccacg ttgtggtgag atttgatgac
     7261 gtgtgcttta ccggttgcag aagcagcaga ttcgataacg tcagggtaga tggtgccctg
     7321 cgccagccac ttcacgtctt ccagtttcag cgcttcttca tcgaatacct caacgaaaac
     7381 gcgaccgatg attttacgtt ttgcttccgg atcgttttcg ccagccagcg ctgacaagaa
     7441 gcgatcttct gccggtacgt gaacaatatt cagaccaaag tgatcgccaa acatatccag
     7501 aacctgctct gcttcgttga gacgcagcag gccgttgtcg acgaatacgc aagtcaggtt
     7561 tttaccgata gcgcggtgca acagcattgc ggtaacggag gaatccacgc caccagagag
     7621 gccgaggatg actttatcgt cgcccacttg ctcgcggatg cgggctacag cgtcgtcgat
     7681 aattttcgct ggcgtccaca gggcttcaca ctggcagata tcacgcacaa aacgctccag
     7741 catgcgcata ccctggcggg tgtgagtcac ttccgggtgg aactgtacgc catagaagcg
     7801 tttttcttcg ttggccatga tggcaaacgg gcagctttcg gtgctggcta cggtgacgaa
     7861 gtcggacggg atagcggtaa ctttatcgcc gtggctcatc cagacgtcca gcagcggttt
     7921 accatctgcg gtcagcgcat cttcgatacc gcgaaccagt gcgctgtcgt ttacgacttc
     7981 aacctgcgcg tagccaaatt cacgttcatt agaagcctca acgtgaccgc ccaactgcat
     8041 cgccatggtc tgcataccat agcaaacgcc gaataccggt acgcctgctt caaagacata
     8101 ctgcggcgca cgcgggctgt tttcttcagt agtactttcc gggccgccgg aaagaataat
     8161 gccgcttgga ttgaagtcac gaatttgtgc ttctgtcaca tcccacgccc acagttcgca
     8221 gtaaacaccc agctcacgca cgcggcgcgc aaccagttga gtgtactgag aaccgaagtc
     8281 cagaatgagg atgcgatgct tatgaatgtt ttccgtcatt gacgcttatt ccgaggcaag
     8341 tgaaacagat aatataaatc gcccgacatg aagtcgggcg aagagaatca ggagcccaga
     8401 cggtagttcg gggactcttt agtaatggtc acgtcgtgaa cgtggctttc ctgaataccc
     8461 gcaccgctga tacgtacaaa ctccgcttta gtacgcagtt cgtcgatagt accacagccg
     8521 gtcaggccca tacaggagcg caggccgccc atctgctggt gaatgatctc tttcaggcga
     8581 cctttatagg ctacgcggcc ttcgatacct tccggcacca gtttgtcggc agcgttgtcg
     8641 ctctggaagt aacggtcaga agaacctttg gacatcgcgc ccagggaacc cataccacgg
     8701 taagatttgt aagaacggcc ctggtagagt tcgatttcac ccggagattc ttcagtacct
     8761 gccagcatgg aacctaccat caccgcgctt gcgccagcgg cgatagcttt agcgatatcg
     8821 cctgagaaac ggataccgcc gtcagcgata accggaatgc cggtaccttc cagggcttct
     8881 actgcatcag caacggcggt gatctgcgga acaccgacac cagtcacgat acgcgtcgta
     8941 cagatagaac cagggccgat acccacttta acggcactgc aaccggcttc tgccagcgcg
     9001 cgggcacctg cagcagttgc cacgttgccg ccgatgattt gcaggtcagg atatttagcg
     9061 cgagtttcac ggatacgctg cagaacacct tcagagtgac cgtgagagga gtcgatcagc
     9121 agaacgtcaa cgcctgcggc aaccagcgca tcgacacgct cttcgttacc agcgccagca
     9181 ccaaccgctg caccaacacg cagacgacct tgttcgtctt tacaggcgtt cggtttacgt
     9241 tctgctttct ggaaatcttt cacggtgatc atgccgatca ggtggaattc atcatcaacc
     9301 accagcgcct tttcaacgcg tttttcgtgc atttttgcca gcaccacttc acgggcttcg
     9361 ccttcacgca cagttaccag acgctctttc ggcgtcatgt aaacgctaac cggttgattc
     9421 agatcggtca caaaacgcac gtcacgaccg gtgatgatgc cgaccagttc gttttcttcg
     9481 gtaacgaccg ggtaacccgc aaagccgtta cgctcggtca gttctttcac ttcacgcagc
     9541 gtcgtggttg gcagtacggt ctgcggatcg gttaccacgc cagattcgtg ttttttcaca
     9601 cggcgaactt cttctgcctg gcgttcaatg gacatgtttt tgtggataaa gccgataccg
     9661 ccttcctgag ccagagcaat agccaggcgc gcttccgtta cggtatccat tgctgcggaa
     9721 agcataggga tattcagacg aatagttttc gtcagctggg tgctgaggtc agcagtattc
     9781 ggcagaacgg tagaatgagc aggaacgagg agaacgtcgt caaacgtcag agcttcttta
     9841 gcgatacgta gcatgggcaa tatctcgacc agagtggtta ataaatattg ccgcggcatt
     9901 atacagagcg taaccgattg catctacccc tttttgcaaa aaatgcttgc tatccccgga
     9961 gggcgggtta ctatcgactg aataacctgc tgatttagaa tttgatctcg ctcacatgtt
    10021 accttctcaa tcccctgcaa tttttaccgt tagccgcctg aatcaaacgg ttcgtctgct
    10081 gcttgagcat gagatggggc aggtgtggat cagcggcgaa atctctaatt ttacacaacc
    10141 agcctccggt cactggtact ttacgcttaa agacgatacc gcgcaggtgc gctgtgcgat
    10201 gttccgcaac agcaaccgcc gggtcacctt ccgcccacag cacgggcaac aggttttagt
    10261 tcgcgccaat attacgctct acgagccgcg cggcgattat caaattatcg tcgaaagtat
    10321 gcagccggcc ggtgaaggat tgctgcaaca gaagtacgaa cagcttaaag cgaagttgca
    10381 gactgaaggt ctgttcgatc tgcaatacaa aaaatcactc ccctcccctg cgcattgcgt
    10441 tggtgtgatc acctcaaaaa ccggtgctgc gctacatgat attttgcatg tgttaaaacg
    10501 tcgcgatcct tctctgccag tgattattta tcccaccgcc gtacaggggg atgatgcgcc
    10561 ggggcaaatc gttcgtgcca ttgaactggc gaataagcgc aacgagtgtg acgtgttgat
    10621 cgttggccgt ggcggtggtt cgctggaaga tttatggagt tttaatgatg agcgtgtggc
    10681 gcgggcgatt tttgccagtc gcattccgat agtcagcgcc gtcgggcatg agacggatgt
    10741 gacgattgcc gactttgttg ctgatctgcg tgcgccaacg ccgtcagccg ccgctgaagt
    10801 ggtgagccga aatcagcaag aattgctacg tcaggtgcaa tcgacccatc aacggctgga
    10861 aatggcgatg gattattatc tcgccaaccg cacgcgtcgt tttacgcaga ttcatcatcg
    10921 cttgcagcaa cagcatccgc agctccggct ggcacgtcag caaactatgc tcgaacgtct
    10981 gcaaaagcgg atgagctttg cgctggagaa tcagcttaaa cgcgcgggtc agcaacagca
    11041 gcgattaaca cggcaactcg tccagcaaaa tccgcagtcg cgtattcatc gcgcgcaaac
    11101 gcgcattcag caactggaat atcgtttagc agaaaccctg cgcgcacagc ttagcgccac
    11161 gcgtgaacgt ttcggtaatg cagtaacaca cctcgaagcc gtaagcccac tgtcaacgct
    11221 ggcgcgcggt tatagcgtca ccagcgccgc tgatggcgcg gtgttaaaac aggttaagca
    11281 agtgaaagtg ggtgagacac tgatcacacg actgggagat ggtgtggtga ttagtgaagt
    11341 cagcgccgta acaaaaaccc gcaaatcacg taaaaaaacg tctaatccgt agaccggata
    11401 agaggcgcca gcatcgcatc cggcaataac tcccaggcta ctccagatcc gccatgctgg
    11461 ttactggaag caggtcacca gacccggttt atttattagc gtgatactac aatcctgtgc
    11521 tccagtacct tcgtccatcc cacactgttg agaggacaaa taaggaaaac tgggtgaaga
    11581 cggccatccc agtgaattaa aaacagtatg caacaactct aattcattgt atgtcggtgc
    11641 attttctatg ctgccacact gccttgcgat atcccattta ctggcatcaa tgatatgtgc
    11701 agaagcccag ttttcgtagt taataatcgg tgaggatacg ggattagccc cggacggcaa
    11761 ttccgtcgtt aacaacggtc gatgtagcga tttactattc accactaccg tgtctgccat
    11821 atgtccccaa aagtgcgccg aggaaacatt tgggctggtt ggtaccgtaa aaatagcgtc
    11881 cagcgtgtct gtcgcggaag cgtaattagc cagagtaacc gtcagtggcg ttttataacc
    11941 aggggtatta tcctggcgga gcgaaaaact tgccgtccca tccgcgcctg tcatgccact
    12001 aatggtatta ccgctttcac tcagcgtaac agccgaacct gataacggct gaacgacgag
    12061 atcgtccatc gccactacat tgccatacaa cgttgcccca gcgttacgcg gcgatgcctc
    12121 accacgtttc aaagtaaatt caacattcgg caccgtattc ccggcgctgt ctttcaccgt
    12181 cacggtaacg gacatggctt cgcctttttt cactttcgcc gcctgagtgg ctgcatcaaa
    12241 cgctgttgag gtaagtgtga cgctggaaac cgttgaacgc gcgttagcca gacaaggctg
    12301 aaatgatttt gttgaacccg tatacagcgt gccatttctc aaatcaaccg actggtcttt
    12361 accatcggcg gatttatcct ctgcccacca ggtgtaatac gcaggccagc cagtttcagt
    12421 gacaaacgtg ttattcggat aacgcgcaga caacgaactt aaatcattga atagtggctg
    12481 ccttgctact tcacaagggc tcacactcgc atctgatttc tgtgttgggg taaagtacgg
    12541 ccagtcttca ttattgttat tgatcttgcc gacgccagaa gtcgatgtca gttcactcca
    12601 caatttcggg cgttcaaata ccgcaccatt cactgccgtc aaggttgctg gcatatgccc
    12661 ccagtagtta gccttcgcag aatccgggct ggtcactacc gtgaatatca ggtcctgatt
    12721 agccgtaacc tgcggatttc gcgccaatga agctgtaaac ggcgttacca gaccaacggt
    12781 gttattttgg gtaacgttga acgttatcgt gccatcactg ccggtgaagc cgctccatgc
    12841 cgacgtattc tggttgaagg agatacgcgc cgatgtcggg gtgacttcat ctaaaatgag
    12901 atcatctacg gtattggagg atacagagat ccctgaacgg cttttcgcct gcacgcgctt
    12961 gagcgtaaca ccttcgccgc caacaggatt accattggca tctttgatcg tcaccacgat
    13021 gggaagcatc tcaccttttt tcacagatga cgtgggggta ccaccattgc tggcagttcg
    13081 gccctcatca taggcagtcg atgacagcgt aaggcttgct ggtgccgaat gtggctgcgt
    13141 cagacagagc attaaagcgt atccattaat agtcgtcgtc gattgtcccg ttttcaaatt
    13201 aatgacgccg taataccacg tcaacgcgtc agtggtgctc atttcataag tccaccaatt
    13261 tccacttccg gcagttatag gcaaccccaa atctgtcagt attgtcccgc ctggatgatc
    13321 attatatagc ccctgtaact cattgagacg cggctgatac tcaaccgggc aatccggcgt
    13381 aactttattg gtataaaaaa ggtatgacca cgactcatta ttattactta ccgaggaccc
    13441 caaggtcgct tctgctgcca gtagcggtcg tttaaaggtc acccccgccc tactggtaaa
    13501 cgtgtcgggc atatgacccc agtattttgc tttcggtgag ttcggactgg ttatcacagt
    13561 gaaaataaca tccatcgtgg cactttttgt ggtgtcgcgc atcaatgttg cagtgagcgg
    13621 agttgccagc ccataagtct cgttttgcgt cacatcaaaa gtcgctttac catcacttcc
    13681 tgtcgtcaga agtgcctgtg ccccattatt atagagtgac atgtttactc cggcaggggt
    13741 gaccggcacg atagtaagat catcatacgt agaatctagc ctgttttttg cacgatccaa
    13801 cgcagtcccg cgtatgagat taaagtttgc gcctggcaac agatttcctc cgctatcccg
    13861 cacaatcacc gtcatccgga tcgccgtacc tttcgccgcc gttgcactag ggcgaccatc
    13921 gttgctggcc gttttctccg catccagtgc cgtggaggtc atctttatag aagctgccac
    13981 tgcatgaggg tttaccagac aggacacaaa tgccacggtg tttgataatg ccctggagct
    14041 tgatccattc gataaattaa tagactgatc gtaccagacg ttcgcgtagg caattttatc
    14101 gtaagcccac caggtgttag tcagcggaag gcctaaatcg gtccctatcg caccattagg
    14161 atgttcactg tataactctt gaaattcgct taataacggt tgataaggtt cgccgcagtc
    14221 tgatttgctg acatcatttt ttgctgccgt attcaaagaa agccatgatt cattattagc
    14281 catggttgac tgccccgctg gatgctcggc agtaagcaat ggccgcttaa aggcaattcc
    14341 cgaccgggta gtaaacgttt caggcatatg cccccagtaa cttgccagtg gcgtatccgg
    14401 gctggttatg acggtaaaca tgaccggtaa tttgttggat tctatcgtca gctgatacaa
    14461 ctgggcgtac atattggtta ccaggcccat agaatcatct tgttttaccg taaaggaggt
    14521 agtcccgtcc gcgccagtca ccccataaag caggcttgaa gaactattca tcctcgcaga
    14581 agcgcctgcc gcgttaatta ccgtaatcga cttatttatc atctcggcat tttgacggtt
    14641 cttcgagcct tcacgtctga gaataaaggg gacattgggc acgctatttc cctgcgcatt
    14701 tttagtcacc acgcgaatca ccgcgtcttc gccttttttc actttcaacg cactcaaggt
    14761 gtcgtcaact ttgacaaatt gcgccgtatc attcgcctca acaatgatct gactggcaac
    14821 ggcgacaggc gtggtccggc agctcagtaa tgcaccacca gatgagggca tctgcgtttc
    14881 atctcctgtc ttcagcgaaa cgacatcata cgtaaaggtt tgagtgaacg tatctgcaac
    14941 ggtattcgag atatagaacc tcgacactgg ccagccaata gcatcatgta atgcgctact
    15001 cccctggtgc gcataaatgg ccctaagttc attgtcagtt ggaacatact ccgcgccaca
    15061 accatttatc gaggtatcgt tagcctggcc cgacgggaac gtagaccagg cctccccctc
    15121 ttccaccagg gtaccgatac tggtggtagc actattctcg tccctcagta aaggccttcg
    15181 ataaataaga ccgtcatcgc ctgtcgccgt ttcagccata tgcccccaga atttggcttt
    15241 tggcgaatcc gggctggtaa gcaccgtaaa gacaacggga agcgtcgatt tagtgctgct
    15301 ggaggaatcc aacgctgccg tgagttctgt ttttagcccg gtagtctggt cttgcttcaa
    15361 tgtgaaagtg gtgatgccat cagcccccgt cacgccataa ataaccaggg ttgttgaaag
    15421 aaaatcatct tgagtatttc cccaggcatc cgttaccgcc agcgaagcaa tcgacgtcgt
    15481 agttgacaca ttggccctgt tcacgctggt gtttcgtttg agggtaaatg cggtatttcc
    15541 cacagggtta ccctgcgcat ctttagtggt cactcgtaat acggcttctt cgcctttttt
    15601 cgctttgaca acattattcg tactgtcaaa ttttgtgggg tctgcagctt caagaacaat
    15661 ttggcttgcc ttaacaatcg gcgtcgtctg gcaggtaagc agttcttttg tggcagatgg
    15721 aattatcgaa ctggtgcctg aactgagatt gacggccttg aagtcacgct gttccaggct
    15781 actactacct gtcgtgctgg aaaggtaact tgaggcgacg ggccagccct gaaccgtttt
    15841 catcgcattg ccgcggttgg cttcatacaa tgactccagc ccggcctggg agggaacata
    15901 ttcactacca caaccattag aggatgtgct tatagcctgg gtttgggtaa attgcgccca
    15961 gtcttcattc tcttctgcaa tcgctgttcg acccgacgta ctggataact ctttcaacaa
    16021 taatgggcgt ttaaagaccg ttccatctgc agccgtcact gtttcaggca tatgccccca
    16081 cattttcgcc ttatcgctgt ccggactggt tacaacggta aaaatggtat cgagggtcgc
    16141 ttttttcgtt gtatctgaat agagcgtagc ggtcagcgac gttttcgtgc catgggtatc
    16201 cgggcgggta atagtgagaa ttttagttcc gtcgctgccg gttatggcgc tgtatgctgc
    16261 agcggtatta ttaagcgaag tttcgtccgc caggccacca ttgaccacaa ccggcgcaac
    16321 cagcgcatca ccgctaccag caacgtgttt ttcctctgag cgggtataac catcgccacg
    16381 tttcagggta aagggtatat cacccaatgg attgccctgc gcatctttta ccgtcacctt
    16441 tacctgtagc gtttcgcctt ttttcagctt cgccgcattt gcagccgcgt tccactgcgc
    16501 cggatctacc acttccagcg taatgctgct ggcaggattg ttagccgtcg tcaggcaact
    16561 gacataaacc gccgtgctac cgccaacggt ccgcgcccca tcgttgaggg ctatggtgta
    16621 ataatgcggt acctgatctg cgggcgaact gctccagtag ggctgacgct gcgttggcca
    16681 gccgtgtact gtctgtacac cgttaccgtt attggcatca tagagcgcgc taagctgaga
    16741 acggcgtggg agcatattgg cgccacagcc acctttactg gtgctggagg cgttggcctg
    16801 gctgactcgc gaccagactt cgttattttc cgttaatgtg ccgtccggac tgaacacttc
    16861 cgcagccagt cgggggcggg caaaggttaa agcatcgacg gtaatcgtct catccatatg
    16921 cccccacatc tgcgcgccaa ccgcatccgg actggtgagc gtagtgaaaa tcacattgta
    16981 attaacagta ttgggaatat aggagttcac aggcacaata tttagcggcg tcgataagcc
    17041 cacaccttgc gattgcttaa tttccacagt cgcaaaaccc tacgcatccg tagtaccggc
    17101 atagactaaa gacggttgcg atgttccgta gagtgtgcca ttcatctcga ttgcgccgcg
    17161 ggttggatca tcaaaacctg tagtctgccc ctgacgattt ttccccatat ctttagtgat
    17221 ggtgaacgcg gtatacggca cgggtgcatt attcaaggca ttaaaggtgc gtaccgtcat
    17281 ggtgatcgtg tcacccactt tggcctgcgc tctgctgcct gcggaaacat cacgatcggt
    17341 ggtcgcggca atcttggcga ccatctcatt gcccgaacaa gagagatagt tttgcttaaa
    17401 gccggaatag gtgtcgatgt tgcccgtggc caggtttacc gacgcgtgcg acgtctcttc
    17461 caccgcacta agatacgctt tttgcaacgt tggccagcca tattcggtgc cgatggcatt
    17521 ggctggatga gcggcaaaca aactgtgcaa tgaactttgg ctcggaatat gacctaaacc
    17581 acactcagcc tgcatactgc tattctgatc aaacagcgcc cagtcttcgt tattttcccg
    17641 caccgatccc agttcgttgt cggtttcatc cgccagccgt gggcgtttaa agatattgtt
    17701 cgcttcaata atgcccaaca tatggcccca catccgcgct ttgtcggtat cggggctggt
    17761 gatcaccgta aaaataacgt ctttctcatc cgaggcagta aaatcactgc gcattctcgc
    17821 ggtgatgtga gttttcaccc ccacgccgcc tggctgggtc agcgtcagag tggcctctcc
    17881 gttagcatca gtgatccctt cataggtatg ggcgtcaact ttgcgaacgt tactgccgcc
    17941 atcaatttgt accggatgcg tctcccacgc ggcgtcagtt tgattcttac ggttaatgcc
    18001 gtcatccaga tgcagcgaaa agtagtagta agccagcggc tgatcgttgt tcttgctatc
    18061 ggtaatcgtc agacgtaaag aagcctcttc ccccaccttt actttcatcg cttttatcgt
    18121 cgaatcatag ctgcccggcg tcagtaccag cttcggttca acatcgggcg cttctttatc
    18181 gacacagctc acaataaacg tcgtatcgtc cggtttttgc gcttcgctgc ggttcgaaac
    18241 atccgccgca tggtgctggc cgctcatccc cgccagtgat gaccagtaga aattaccctg
    18301 catcggccaa ccagccacat cctgcactga cgttggcacc accgtcgcca gcgcaccgaa
    18361 ctgacgcatt ccgggcaaaa tgtcgcaatg tttatccgcg ccgctccagg taaaggtgga
    18421 ccaggtttca ttgtgatcgt taaccgtacc gccttcatta tcgacttccg ccgccagttt
    18481 tggtcgacta aaagtgtaat cacgggcctt cagcgtatcg ggcatatgac cccacattgt
    18541 agcctgcgga acatccgggc tggtgaggac ggtaaagatc actgccgttt cactggcctg
    18601 agcaataccc gcgaggctcg cgaccaacgg cgtttttaca ccagggccgt tggcctgggt
    18661 gaccgtaacc gtggcaacac cattcgcatc ggtagtacca cgatattccg tcgcggtggt
    18721 ggttaattcg gtcgtgccca gttttacagg cgcagtattg ttcaccacac cctggcggtt
    18781 ttcagcatct ttgcgtttga taacaaaagg aatattcccc acaacattac cgacgcaatc
    18841 tttggtggtg accgttaacg tgatactgcc cccaaccgtc attttcgccg cacctgagga
    18901 ttcgtcatag cccgcagcgc tgacggaaat aaggtcaccc tgcgcgggcg caatcttcag
    18961 cgtcactttc tgatcggcga gcgtcagaac actttcatag ggatagcccg tcgaggtgag
    19021 taaatataac agcggcatgg tctgctgggg atcaggctgc gcccaggcga ccagcgaacg
    19081 cccggcaagt ccctgtgcac aaaacgagcg atctgcgact ggctgtgggt ccagtaaggt
    19141 gtccggcgtg gcggcatccg cccaaactaa agatacagcc ggtggcgttt ggttttccca
    19201 acgtggggtg gcaatgatat cgccttctct gtcttgcggg ttggtgacga gcaatttaga
    19261 gacgctaaca tccgcgttga attcactcgg tttagcggta aacgctacct catgcgtctt
    19321 tgtaggatcc agaaagacac tgccctggta gacggatatg ctggcgctgt cggcggtggg
    19381 cacggtaccg ttaaattcgc cgaccgtatc tgaagagtct tgccatgcag attctgccca
    19441 tgaccaccca ggcagcattg cgcttatcag caatgcgcat aacacaagga ttttattcag
    19501 ggtcagggac atgacagaaa ctccaccggt tgttaaacgg acgcttgtcg catcaatata
    19561 ttttgcttaa tccgggatta agcaaaatat gcggaaacag cattcccttg cgggaatgct
    19621 gctgagtacg cgttatttgt gtttgtaaat aatgtgaagt ttgtaaccct gtaccccgtc
    19681 gccgttggtc gggatggaaa gtgcctcttt tccatcgcgc gcagggccgt taaggttggt
    19741 tctggcttcg tcgttggtcg cagggatgac caaattgtta ttatcgaagc ttgagttaac
    19801 gatgccaccc gaagtgccaa gctgttggct gctgccagaa agtacccaac ggaagtcata
    19861 attggcggtg acgtcagcat cggtacccgc atcaaacttg ccatttttgt tctcatccga
    19921 gtacagtttg gccacgtagg ttttgccaag ttcgattggt gtgctaccgt tgataaggtt
    19981 gatactggta ccatcaacaa agatggcgac tttcaggttc tggttaacca ccggccccgg
    20041 tgcaacgtta tcgctgtcgc ttccgccgcc gctgacagta gaaacgtcat aaagatgcag
    20101 cgcagtcccg acgtttggcg taccggtctg cgtggttggc gtaatttcaa taccgatata
    20161 gcgaccacgg tcggcatcgg taagagtgta actggtcacc ttcgttgcca gctcttttgg
    20221 atcccccccc tgcgcatcct tgtagctcat ccagcggata gtatcgatag ttttcgcgtt
    20281 atcgcccaat cctccaagat ccacgtcgcc ttcggtatca ccaatttccc aacttacggt
    20341 taatttatca cccgagtaaa gacgcttgtc cgtttcactg ccttgcgggc gagcgccttt
    20401 tagatcagaa gtcaacgtta cacggtcggc atctgtcgta gcacttcccc cttccgaata
    20461 gagccacgga atggtcccct ggaaaatatt ggtttcaccg ctgtcgaccc aggcgctgtc
    20521 ttcagccagc gccacaggtg ccacacaata acctgccagt gccagcgcta atgcgatccg
    20581 ggtcagaccg cgtcttttat cctgcttcat tgatgctctc cttaatgaac cttcagcgac
    20641 tggccttacg ccaatcttat tttttggtat acagcacgcg taaaccgtac ccctgcaagc
    20701 catctcccgc ttgtgcgcca taggtttgcg ccgcttcacg gttagtggcc ggaatcacaa
    20761 tgtcctcatt ggaagtgttc gcctgtgcac ccacttcacc attgatgctc ttgccggtaa
    20821 attcccactt gtagtcgtac tgcgccatct cttcgtcagt cagtttttcg acctgctgga
    20881 agacaccgtc tttattgttg tcgcgccata aaacaaatcg ataaatgttg ttaagcggca
    20941 acggttcttt atctttacct atcaggttgc tgggtttagc cgcccccact cgatagataa
    21001 aagcatttag cccaccaatt tctgccccga ggaaagtgac atccacatag ttactgtcat
    21061 cgtagggcgc ggcttttgct ttccagcgat aggtgccggg taaggtactg gagagggtga
    21121 cagtgccctt ttctttatct tgtttagcga taaaaacgca ggggttgtcg gtcgagcaag
    21181 gcttgttggt taacgtcact cgtttgtcct gcgcgtcgta aagctgcggc gtgaggttat
    21241 cttccggcag cgcatcgcca ttgcggtcgg tgttatcgcc aaagctgtcg gttacatcga
    21301 gatccatcac cacaccatca acgccatctg cctgagcgct ggcttcgtgg ctgtggttgt
    21361 tctcttctgg cacgttcgcg atgttattaa caatcaactc tattttacgg tcctgctgaa
    21421 cggtgatttc caccacgcca gagttctgtc ggttgccctt ttcatcttcc agcgccagac
    21481 gtactcgcca ggtattgagt ttttcttgtt cggcttgatc ggaactgtac tgccaggcag
    21541 gcagcaccag gttccagtgg ttattgttac cctctgtcag agtattctct ttgataaccg
    21601 gcatcaccag cgtgcgggca gcattgtttt tcgcctgcca agaggcagca acgattttgt
    21661 aatgatgatt aatgagtgcg ttaatggtcc acttacattt ttccagaccg acggcttctt
    21721 cgggcgtatc tttaatcacg cactcgggat gttcattcgt gacgtcggct ttcagccaca
    21781 aagtgacata gaggggatct ttctctttgt attccagaac aataaagttg ttacgttcaa
    21841 caaagtcatg acggctaccc atgagcgagt gagccgcgct cactttgtca ggatcaagct
    21901 ggtctttgag cgggacgcca aactggtagt tcagcgttgc gttaacagaa agatcggtat
    21961 tatcgccagt gccagctttg aaatctgtac cgacaacaat gagcggaacg ggctggtatt
    22021 tcacacccag cgtgacagcg aagggatctt tctccagact atcggtacca aaaagcgcga
    22081 cttcattccc ataatattgt tcgaagacaa tttttccgcc aagctgcggg taagcgggta
    22141 gccaggcttc agcgcgaata tcccagccgc gcgcagggcg ttcttcataa aagtcgaaat
    22201 cttcagagtc tttccagtca gaaagtgggt gataatagtt gcctgagaat tttaaataat
    22261 ccgtccaggc ttcggcgcct aaacctaaac ggcgatgtcc acgggtaaag tcataatcat
    22321 aaaatatatt tccacccaat agatatttat caaaattata ccttacccca aggccaatat
    22381 tcccgatcgt acgatcttct tttcgttgcg cagaaaattg gctgaaataa acagtggttt
    22441 gattatcgta ccagggaaca aaataatcga tggaactgcc atccagatcg ccgccctgac
    22501 caatggagag gttcgaacgg accttaccgt agggagagag tgtgtcttca atataagatt
    22561 gtgcggttga ggtgatttga ctttgcagat agcttctggc ctgagattcc agcgcctcag
    22621 gggtcaagtt ttcaaaccct tgcgtggcgg agttgatgac gtaatcggct ccgcgctctt
    22681 tcagcgattt acccttgttg gtttgttcat cctgttgtgc tgcctggcta cctaaatcag
    22741 gtaacgattc gttttgctta acatttatct ctggcgccgc gaaagcagca ccagagataa
    22801 atgttagaat aatacagcgt ttccatcgca gcatgcactt tccctgtgac aatgatacag
    22861 tcaaaaataa gctttaatat tgtatccctg attattcaga aaggcatgtc ataaagagta
    22921 aatatattct taa
//
LOCUS       DAZGJT010000077        22755 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZGJT010000077 DAZGJT010000000
VERSION     DAZGJT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.077"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            555..1004
                     /gene="alaE"
                     /locus_tag="ACHKFK_003263"
     CDS             555..1004
                     /gene="alaE"
                     /locus_tag="ACHKFK_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO9053535.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILVYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(1041..1385)
                     /locus_tag="ACHKFK_003264"
     CDS             complement(1041..1385)
                     /locus_tag="ACHKFK_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO9053536.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1537..1866
                     /locus_tag="ACHKFK_003265"
     CDS             1537..1866
                     /locus_tag="ACHKFK_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO9053537.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            complement(1923..2666)
                     /locus_tag="ACHKFK_003266"
                     /pseudo
     CDS             complement(1923..2666)
                     /locus_tag="ACHKFK_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
     gene            2756..3187
                     /locus_tag="ACHKFK_003267"
     CDS             2756..3187
                     /locus_tag="ACHKFK_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO9053538.1"
                     /translation="MTTLRQPYYELSPAVYNALVQAKTALENSTLDTTLMELVYLRVS
                     QINGCAFCLEMHSKALRKSGVPQHKLDALAGWRVSHHFDERERAALAWAESVTEIART
                     HAEDEVYQPLLEHFSAAEISDLTFAIGLMNCFNRLAVSMRM"
     gene            3396..3641
                     /gene="nrdH"
                     /locus_tag="ACHKFK_003268"
     CDS             3396..3641
                     /gene="nrdH"
                     /locus_tag="ACHKFK_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO9053539.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3638..4048
                     /gene="nrdI"
                     /locus_tag="ACHKFK_003269"
     CDS             3638..4048
                     /gene="nrdI"
                     /locus_tag="ACHKFK_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO9053540.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4021..6165
                     /gene="nrdE"
                     /locus_tag="ACHKFK_003270"
     CDS             4021..6165
                     /gene="nrdE"
                     /locus_tag="ACHKFK_003270"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9053541.1"
                     /translation="MATTTAERLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MTTHVRPNSVAFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHASGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLATQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     VQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6175..7134
                     /gene="nrdF"
                     /locus_tag="ACHKFK_003271"
     CDS             6175..7134
                     /gene="nrdF"
                     /locus_tag="ACHKFK_003271"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9053542.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLSVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFASRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNQEKISQAQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWSDDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7490..8692
                     /gene="proV"
                     /locus_tag="ACHKFK_003272"
     CDS             7490..8692
                     /gene="proV"
                     /locus_tag="ACHKFK_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO9053543.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKAALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8685..9749
                     /gene="proW"
                     /locus_tag="ACHKFK_003273"
     CDS             8685..9749
                     /gene="proW"
                     /locus_tag="ACHKFK_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO9053544.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            9806..10798
                     /gene="proX"
                     /locus_tag="ACHKFK_003274"
     CDS             9806..10798
                     /gene="proX"
                     /locus_tag="ACHKFK_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO9053545.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYGLTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            10990..12177
                     /locus_tag="ACHKFK_003275"
     CDS             10990..12177
                     /locus_tag="ACHKFK_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053546.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASMLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISNKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDA
                     RNRLTAGYMTSYFIGGAAGSLISASAWQHGSWAGVCLAGATIALVNLLVWWRGFHRQE
                     AAN"
     gene            12301..13038
                     /gene="ygaZ"
                     /locus_tag="ACHKFK_003276"
     CDS             12301..13038
                     /gene="ygaZ"
                     /locus_tag="ACHKFK_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO9053547.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13028..13363
                     /gene="ygaH"
                     /locus_tag="ACHKFK_003277"
     CDS             13028..13363
                     /gene="ygaH"
                     /locus_tag="ACHKFK_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO9053548.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13454..13984
                     /gene="emrR"
                     /locus_tag="ACHKFK_003278"
     CDS             13454..13984
                     /gene="emrR"
                     /locus_tag="ACHKFK_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO9053549.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14111..15283
                     /gene="emrA"
                     /locus_tag="ACHKFK_003279"
     CDS             14111..15283
                     /gene="emrA"
                     /locus_tag="ACHKFK_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO9053550.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHTRDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            15300..16838
                     /gene="emrB"
                     /locus_tag="ACHKFK_003280"
     CDS             15300..16838
                     /gene="emrB"
                     /locus_tag="ACHKFK_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO9053551.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESLHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            17096..17803
                     /locus_tag="ACHKFK_003281"
     CDS             17096..17803
                     /locus_tag="ACHKFK_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HFO9053552.1"
                     /translation="MNTQRKYGRTWHYPFSPGTTSDDRINTDYWQDLKAITQLVHTEK
                     LDGENNCLNRYGVFARSHATPTQSAWTYKIRQRWQLLKNDLGDLELFGENLYAVHSIE
                     YRALEQDFYLFAVRCQDMWLSWEEVQFYAALFDFPCVPEISGPQPGNDEKSWQRDFLA
                     LTNARGTFDPWDTQTCQPCTLEGIVSRNHDAFSVADFSHNVFKYVRKNHVKTTVHWKR
                     HWQRARMAHEFVYGEQS"
     gene            17800..18912
                     /locus_tag="ACHKFK_003282"
     CDS             17800..18912
                     /locus_tag="ACHKFK_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9053553.1"
                     /translation="MIWQLTDDKRWSALRQRFSWVEEMHHTPQDPEHHGEGDVGVHTE
                     MVLNALITLPEFQQLPAQQQEVLWAAALLHDVEKRSTTVQENGRIQSPGHARRGELTA
                     RQILWRDIPTPFVLREQIVALVRLHRLPLWLLERPEPERLLLAAAMRIDTRLLALLAR
                     ADLLGRQSPDQQSMLERIDLFELFCHEQQCWGKMRPFVSDSARWHYLTHEQSSPDFVP
                     WEAEPFEVILLCGLPGMGKDRYINEQCQGMDVISLDDMRRRINASPDDKTATGRIVQQ
                     AKEEARVFLRQKKPFIWNATNITRQLRSQLISLFTAYGARVKIVYLEVPWAQWKQQNA
                     RREYAVPEAVVMRMASRLEVPQPDEAHSVEYRMTDR"
     gene            complement(18963..19478)
                     /gene="luxS"
                     /locus_tag="ACHKFK_003283"
     CDS             complement(18963..19478)
                     /gene="luxS"
                     /locus_tag="ACHKFK_003283"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO9053554.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARNILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(19628..21184)
                     /gene="gshA"
                     /locus_tag="ACHKFK_003284"
     CDS             complement(19628..21184)
                     /gene="gshA"
                     /locus_tag="ACHKFK_003284"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO9053555.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(21257..21685)
                     /locus_tag="ACHKFK_003285"
     CDS             complement(21257..21685)
                     /locus_tag="ACHKFK_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO9053556.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGVSHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(21682..22248)
                     /gene="yqaB"
                     /locus_tag="ACHKFK_003286"
     CDS             complement(21682..22248)
                     /gene="yqaB"
                     /locus_tag="ACHKFK_003286"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO9053557.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGYYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(22529..22605)
                     /locus_tag="ACHKFK_003287"
     tRNA            complement(22529..22605)
                     /locus_tag="ACHKFK_003287"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22569..22571),aa:Arg,seq:acg)
     gene            complement(22669..22745)
                     /locus_tag="ACHKFK_003288"
     tRNA            complement(22669..22745)
                     /locus_tag="ACHKFK_003288"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22709..22711),aa:Arg,seq:acg)
BASE COUNT         5476 a         5896 c         5724 g         5659 t
ORIGIN      
        1 tttcagctgt tattttctga aactctcaga ataatttcaa gattatatat tagccatttt
       61 gatgagtaat ttcggttatt ggtgaacaga gcggctctct ctgttcgata attccgcaag
      121 cctgacaaaa ccaaagtgaa taaaaatgca atttaaccga ataaaagaaa acatctatca
      181 ctggtgcttt ctgctgtcaa tgtacattat ctggcataaa aaatgctgaa aatgacccgg
      241 tcactacata caccgaattc tctacgctta tagcgttacc tcacacccaa accttcatga
      301 gtgaaaacaa aggcaaacac atatccaatt gatttaatta aaaataaatc ataccggcat
      361 gtaaaaatcc acaatgtaca aaaaacgcgc cgttacggaa tattttatct gcaaaaacag
      421 attaaataaa aatttttcac taattgatta gtcatagcca gcgatatacg ctatgcgaaa
      481 atgcagatgg caatgagatc cgctgctttc atctccatta acatcccatt acgcttttat
      541 taaggagcat tagcatgttc tcaccgcagt cacgcttgcg tcatgcagtt gcagatacgt
      601 tcgcgatggt tgtttactgt tctgtcgtga acatgtgtat tgaagttttc ctctccggaa
      661 tgagcttcga acagtctttt tattccagat tggtagcgat tccggtgaac atcttaattg
      721 catggccata cggtatgtac cgcgatctgt ttatgcgcgc ggcacgcaaa gttagcccgt
      781 cgggctggat aaaaaatctg gcggatatcc tggtttatgt gacgttccag tcaccggtgt
      841 atgtggcgat cttgttagtg gtgggcgcag actggcatca gattatggcg gcagtcagtt
      901 caaacatcgt tgtttcgatg ttgatggggg cggtttatgg ctacttcctc gattattgcc
      961 gccgactgtt taaagtcagc cgttaccagc aggtaaaagc ctgacccttc ttttcgcgac
     1021 tggcatcgcc agtcgcgtct ttaactggct tcgccaaata aaccattcaa ataacgttca
     1081 agcgcaacac gcgaactaaa gccatgcggg ataccgtaat gctcatgacg ttcacccgct
     1141 aaatagagcg gaaattgctg gtaatgatcg caagttttaa tatcggaagc gggttcagcc
     1201 agtgttgaac tacgttcttt taatgttgga tgaatcacca gggcggtacg tcccattcgc
     1261 gcttcgcgat taacatagac ataattttcc ccacggcggt aaccatacgc tttctgggtt
     1321 accacatcga cagtaaaacc gactttttca agtacgcgcg ccacctcgtc tggtcgtaaa
     1381 tacatatttg atcctcgtta ttatcaaccg cgggcctacc ttacctgatt gcgcattcac
     1441 aacgctttca gaaaagtcca taaagccgcg atgaacctca gttaagaaaa ttatggtcta
     1501 cactgaaaat tacatcgaat tctaatgggg gatcatatgt ttaaccgacc gaaccgcaac
     1561 gacgttgatg atggcgtgca ggatatccag aatgatgtca atcaattagc tgacagtctc
     1621 gaatctgtat tgaagtcctg gggcagcgac gccaaagggg aagccgaagc cgctcgtagt
     1681 aaagcgcagg ctctactgaa agaaaccagg gcgcgaatgc atggtcgtac tcgcgtccag
     1741 caagccgcgc gcgatgccgt tggctgcgcg gattcttttg ttcgtgaaag accctggtgt
     1801 agtgtgggta cagcggcagc ggtgggtatt tttatcggcg cactgttaag catgcgcaaa
     1861 tcgtagtgca aaaatgataa taaatacgcg tctttgaccc cgaagcctgg cttcggggtt
     1921 ttttagctta aggctttacg taattgttgt gctacctgtc gcgccatacc ttcgttaacg
     1981 atattagtaa acgaaagcag taatccacct tccccacttg aacggatacg ccagtcgcta
     2041 agtgcctgca ccgcaaggcc tgcagcattg gctttacgcg catgggcaat atcatcgcct
     2101 atcattctga tcaccatctg gataccacct ttctgcggca caacctgaaa tccttgctgc
     2161 gtcaacgctt gctcaatcca ttgccgacgt tgagcataat gctgacgcat tttcttcaga
     2221 tgccgccaga aatgcccctc gcgaagaaag tctgccagtg tgttctgcca tagaacaggt
     2281 accgcacatg gagccagtga cgcctggtgg cggaattgtg caatttgctt caccggcacc
     2341 accagccacg cacagcgcaa tgcaggaaat agcgctttgc tgaatgttcc ggcataaatt
     2401 acccgctgcg gtgcgtcgag actttttaac gccggtaacg gcttaccgtg atagcgaaac
     2461 tcactgtcat aatcatcttc aataatccat gcctgactac gatctgccca ttccagtatc
     2521 tgatgcctgc gcgctaaaga gagcgccaca cccagcggac tttggtgtgc tggtgttatc
     2581 agggcaaaac gcgcatcagg ataattttgt attccgcttg tgatatccag tccattgtca
     2641 tcaaccggca caggcaaaat ttccacatct ggtctgcttt tttaggtaat ttatggctct
     2701 ctttaacagt ccattatagt gctacattgc gggcgttaat aagaggagaa actgtatgac
     2761 tacattacgt cagccttact acgaacttag cccggcagtg tataacgcac tagtgcaggc
     2821 caaaacggca ctggaaaata gcacgctgga taccacgttg atggagttag tttatttgcg
     2881 cgtctcgcaa atcaacggct gcgcattttg tctggagatg cacagcaaag cattgcgcaa
     2941 atccggcgtg ccacagcaca aactggacgc cctggcaggt tggcgcgtaa gccatcattt
     3001 tgatgaacgc gagcgggcgg cgctggcatg ggcagaatcg gtaaccgaaa ttgccagaac
     3061 ccatgcggaa gacgaggttt atcagccttt gcttgagcat ttcagcgcag cagagatcag
     3121 cgacttaacg tttgccatcg ggctgatgaa ttgttttaac cgtctggccg tttccatgcg
     3181 gatgtaactt cttgatgccg ggtattcgcc cggcaaaaaa acacaaaatc ccctaccccg
     3241 ttgagccatt catcggtggc gtaatcaatc taaaattccc atatcttgtg tggttgaatc
     3301 ttttttcaac tacatctagt atctctgtat caacagagag acaacccgac gcgtatcatc
     3361 gcgccgtatc ttcattttaa acggaaatac gaatcatgcg cattactatt tacactcgta
     3421 acgattgcgt tcagtgccac gccaccaaac gggcgatgga aaaccggggc tttgattttg
     3481 aaatgattaa tgtcgatcgc gttcctgaag cggcagaagc gctgcgtgct cagggctttc
     3541 gtcagttgcc ggttgtgatt gcaggcgatc ttagctggtc tggtttccgc ccggacatga
     3601 tcaatcgtct gcatccagcg ccacacgcgg ccagtgcatg agccagctcg tctacttttc
     3661 cagcagctcc gaaaatacgc agcgttttat cgaacgttta gggctgcccg cggtgcgcat
     3721 cccgctcaat gagcgggaac ggattcaggt agacgagcct tacatcctga tcgtgccctc
     3781 ttacggcggc ggcggtacgg ctggtgcggt gccacgacag gtcattcgct ttttaaacga
     3841 cgagcataac cgggcgttgc tgcgcggcgt tattgcttcg ggtaatcgca actttggtga
     3901 ggcatatggc cgcgccggag atgtgattgc ccggaaatgc ggcgtgccgt ggctgtaccg
     3961 ctttgaactc atgggtacgc aaagcgatat cgaaaacgtt cgtaaaggag taaccgaatt
     4021 ttggcaacga caaccgcaga acgcctgacg caggaaacga tggattatca cgctctgaat
     4081 gcgatgctta acctttacga tagcgcaggt cgcattcagt tcgataaaga tcgccaggca
     4141 gtagacgcct ttatgacgac gcatgtgcgt ccgaacagtg tggccttcag tagccagcag
     4201 cagcgcctga actggctggt taacgaaggt tactatgatg aaagcgtcct taatcgctac
     4261 tctcgcgatt ttgtcattac gctgtttgcc cacgcgcacg ccagcggttt tcgtttccag
     4321 acattcctcg gagcatggaa gttttacacc agctatacgt tgaagacatt cgacggtaag
     4381 cgttatctgg aagattttgc cgatcgcgtc acgatggtgg cgctgacgct ggcacaaggc
     4441 gatgaaacac tggcgacgca gctcactgat gagatgctgt caggacgctt tcagccagcc
     4501 acgccaacat tcctcaactg cggtaagcag cagcgcggcg aactggtttc ctgttttctg
     4561 ctgcgtattg aagacaatat ggagtcaatt ggccgggcag tgaattccgc actacaactg
     4621 tcgaaacgcg gcggcggcgt agcatttttg ctgtcgaatc tgcgggaagc gggcgcgcca
     4681 attaaacgta ttgaaaatca atcttctggc gtgattccgg tgatgaaaat gctggaagac
     4741 gcattttcct atgccaacca actcggcgcg cgtcaggggg ctggggcagt ctatttacat
     4801 gctcatcatc ccgatattct gcgttttctc gatacaaaac gggaaaatgc cgacgaaaaa
     4861 atccgcatta aaacactgtc gctgggcgtg gtgatcccgg atatcacttt ccatctggca
     4921 aaagagaatg cgcagatggc gctgttttcg ccctatgacg tagagcgagt ttatggcaag
     4981 ccgtttgccg atgtcgccat cagtgaacac tatgacgaac tggttgccga tgaacgcatc
     5041 cgcaaaaaat acctcaacgc ccgtgatttc ttccagcgac tggcagaaat ccagtttgag
     5101 tctggctatc cgtacatcat gtatgaagac acggttaacc gtgctaaccc tatcgccggg
     5161 cgcataaata tgagcaatct ctgctcagaa attttgcagg ttaacagcgc ctcagaatat
     5221 gacgagaatc tcgactatgc tcgtacagga catgatattt cctgcaatct agggtcgttg
     5281 aatattgccc acaccatgga ttcccccgac tttgcccgca cggtagagac tgccgtacgc
     5341 ggtttaacgg cggtgtcaga tatgagtcat atccgcagcg tgccgtccat cgaagccgga
     5401 aatgccgcct cacacgccat cggtctgggg cagatgaatt tacacggtta tctggcacga
     5461 gaaggcatcg cctatgggtc gccggaagca ctggatttca ccaatctcta tttctatacc
     5521 atcacctggc acgcactgcg tacctcgatg ttgctggcgc gcgaacgcgg tgaaaccttc
     5581 gccgggttca aacagtcacg ctatgccagt ggtgaatatt tcagccaata tctgcaaggt
     5641 aactggcagc cgaaaacggc gaaagttggc gaactgtttg cccgtagcgg tattacgtta
     5701 cctacccgtg agatgtgggt gcagctgcgc gacgacgtga tgcgctatgg catatacaac
     5761 cagaatctcc aggcggtgcc gccaaccggt tctatctctt atatcaacca tgccacgtcg
     5821 agtattcatc cgattgtggc gaaagtagag atacgcaaag agggcaaaac aggacgcgtt
     5881 tactaccctg ccccgtttat gactaacgaa aatctggcgc tgtatcagga cgcttacgaa
     5941 atcggcgcag aaaagatcat cgacacctac gcggaagcga ctcgccatgt cgatcagggg
     6001 ctgtcgctga cgctgttttt ccccgatacc gccaccactc gcgatatcaa caaagcgcag
     6061 atttacgcct ggcgcaaggg tatcaaaacg ctctattaca ttcgcctgcg tcagatggcg
     6121 ctggaaggca ctgaaattga aggctgcgtc tcctgtgcac tttaaggaat atctatgaaa
     6181 ctctcacgta tcagcgccat caactggaac aagatatctg acgataaaga tctggaggtg
     6241 tggaatcgcc tgaccagcaa tttctggcta ccagaaaagg tgccactgtc gaacgatatt
     6301 cctgcatggc agacattaag tgtcgtagaa caacaactaa caatgcgcgt ttttactggc
     6361 ctgacgctgc tcgacacgct gcaaaatgtt atcggcgcgc cctcgctgat gcccgatgca
     6421 ctcacccccc atgaagaggc ggtattatca aatatcagct ttatggaagc ggttcatgcc
     6481 cgctcttaca gttcaatttt ctcgacgctg tgccagacca aagatgtcga tgccgcctac
     6541 gcctggagcg aagaaaacgc gccgttgcag cgaaaagcac agattattca gcaacattat
     6601 cgcggtgatg atccgctgaa aaagaaaatc gccagtgtgt ttcttgaatc ttttttgttc
     6661 tattccggtt tctggctgcc gatgtatttt gccagccgcg gaaagctaac caataccgca
     6721 gacctgatcc gtctgattat ccgggatgaa gcagtccacg gttactacat cggctataaa
     6781 tatcagaaaa accaggaaaa gatctcccag gcacagcgtg aagagttgaa gagtttcgcc
     6841 ttcgatttgt tgctggaact ctatgacaac gagttgcaat ataccgatga gctgtacgcc
     6901 gaaaccccgt ggtctgacga tgtaaaagcg tttctctgtt acaacgccaa taaggcgttg
     6961 atgaatctgg gctacgaacc gctatttccc gcagaaatgg cggaagtgaa tccggcaatc
     7021 ctcgccgcgc tttcgccgaa tgccgatgaa aatcacgatt tcttttccgg ttcaggctct
     7081 tcatatgtga tgggaaaagc ggtcgaaaca gaagatgaag actggaattt ctgagggggt
     7141 tattttcaaa aatatcacta cccgctgcag ggaaataatt cccgccaaat agctttatat
     7201 cacgtaaata atttgtggtg atctacactg atactctgtt gcattattcg cctgaaacca
     7261 caatattcag gcgttttttt gctatctttg acaaaaaata tcaacttttc tcgatttgct
     7321 ctcagccctt atatcatggg aaattccggc gatttgctca catcaatatt catgccacat
     7381 ttgccctcag gggttgcctc agattctcag tatgttaggg tagaaaaaag tgactatttc
     7441 cattgggtaa tgtatcgaca taaacaaata ataggaatct ttctattgca tggcaattaa
     7501 attagaaatt aaaaatcttt ataaaatatt tggtgagcat ccacagcgag ctttcaaata
     7561 tatcgaacaa ggactttcaa aagaacaaat tctggaaaaa actgggctat cgcttggcgt
     7621 aaaagacgcc agtctggcca ttgaagaagg cgagatattt gtcatcatgg gattatccgg
     7681 ttcgggtaaa tccacaatgg tacgccttct caatcgcctg attgaaccca cccgcgggca
     7741 agtgctgatt gatggtgtgg atattgccaa aatatccgac gccgaactcc gtgaggtgcg
     7801 cagaaaaaag attgcgatgg tcttccagtc ctttgcctta atgccgcata tgaccgtgct
     7861 ggacaatact gcgttcggta tggaactggc cggaattaat gccgaagaac gccgggaaaa
     7921 ggcccttgat gcactgcgtc aggtcgggct ggaaaattat gcccacagct acccggatga
     7981 actctctggc gggatgcgtc aacgagtggg attagcccgc gcgttagcga ttaatccgga
     8041 tatattatta atggacgaag ccttctcggc gctcgatcca ttaattcgca ccgagatgca
     8101 ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc attgtcttta tttcccacga
     8161 tctcgatgaa gctatgcgta ttggtgaccg aattgccatt atgcaaaatg gtgaagtggt
     8221 gcaggttggt acaccggatg aaattcttaa taatccggcg aatgattatg tccgaacctt
     8281 cttccgtggc gtcgatatta gccaggtgtt cagtgcgaaa gatattgccc gtcgtacacc
     8341 gaacggctta attcgtaaaa cccccggctt cggcccgcgt tcggcactga aattattgca
     8401 ggatgaagat cgcgaatatg gctacgtcat cgaacgcggt aataagtttg tcggcgctgt
     8461 gtctatcgat tcgcttaaag ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat
     8521 tgatgcgccg ttagcggtcg atgcacaaac gcctcttagc gaattgctct ctcatgtcgg
     8581 acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc
     8641 gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta aataatggct gatcaaaata
     8701 atccgtggga taccacgcca gcggcggaca gtgctgcaca atccgcagac gcctggggta
     8761 caccggcgac tgcaccgact gacggcggtg gcgctgactg gctgaccagt acgcctgcgc
     8821 caaacgtcga gcattttaat attctcgatc cgttccataa aacgctgatc ccgctcgaca
     8881 gttgggtcac tgaagggatc gactgggtcg ttacccattt tcgtcccgtc tttcagggcg
     8941 tgcgcgttcc ggttgattac atcctcaacg gtttccagca attgctgctg ggtatgcccg
     9001 cgccggtggc gattatcgtt ttcgctctca tcgcctggca gatttccggg gtcggaatgg
     9061 gcgtggcgac gctggtttcg ctgattgcca tcggcgcaat cggtgcctgg tcacaggcaa
     9121 tggttaccct ggcgctggtg ttaacagccc tgctgttctg tatcgtcata ggtttgccgt
     9181 tggggatctg gctggcgaga agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg
     9241 ccatgcagac cacgcccgcg tttgtttatc tggtgccaat cgtcatgctg tttggtatcg
     9301 gcaacgtgcc aggcgtggtg gtgacgatca tctttgcgct gccgccgatt atccgtctga
     9361 cgattctggg aattaaccag gttccggcgg atctgattga agcctcgcgc tcattcggtg
     9421 ccagcccgcg ccagatgctg ttcaaagttc agttaccgct ggcgatgcca accattatgg
     9481 cgggcgttaa ccagacgctg atgctggccc tttctatggt ggtcatcgcc tcgatgattg
     9541 ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg tcgtctggat atggggcttg
     9601 ccaccgttgg cggcgtcggg attgtgatcc tcgccattat cctcgaccgc ctgacgcagg
     9661 ccgttgggcg cgactcacgc agtcgcggca atcgtcgctg gtacaccact ggccctgtcg
     9721 gtctgctgac ccgcccattc attaagtaac tctgcacttg cccggtgacg ccgggcatat
     9781 caccctgcca aaaaaaggaa taacaatgcg acatagcgta ctttttgcga cagcgtttgc
     9841 cacgcttatc tctacacaaa cttttgctgc cgacctgccg ggcaaaggca ttactgttaa
     9901 tccagttcaa agcaccatca ccgaagaaac cttccagaca ctgctggtaa gtcgtgcgct
     9961 ggagaaatta ggttataccg tcaataaacc cagcgaagtg gattacaacg ttggctacac
    10021 ctcgcttgct tccggcgatg caaccttcac cgcagtgaac tggacgccac tgcatgacaa
    10081 catgtacgaa gctgccggtg gcgataagaa attttatcgt gaaggagtat ttgttaacgg
    10141 cgcggcacag ggttacctga tcgataagaa aaccgccgac cagtataaaa ttaccaacat
    10201 cgcgcaactt aaagatccga agatcgccaa actgttcgat accaacggcg acggtaaagc
    10261 ggatttaacc ggctgtaacc caggctgggg ctgcgaaggt gcgatcaacc accagcttgc
    10321 cgcgtatgga ctgaccaata ccgtgacgca taatcagggg aactacgcag cgatgatggc
    10381 tgacaccatc agtcgttaca aagagggtaa gccggtgttt tactacacct ggacgccgta
    10441 ctgggtgagt aacgaactga aaccggggaa agatgtggtc tggttgcagg tgccgttctc
    10501 cgcactgccg ggcgataaaa atgccgatac caaactgccg aatggcgcga attacggctt
    10561 cccggtcagt accatgcata tcgttgccaa caaagcctgg gccgagaaaa acccggcagc
    10621 agcgaaactg tttgccatta tgcagttacc agtggcagat attaacgccc agaacgccat
    10681 tatgcatgac ggcaaagcct cagaaggcga tattcagggc cacgttgatg gttggatcaa
    10741 agcccaccag cagcagttcg atggctgggt gaatgaagcg ctggcagcgc agaagtaatt
    10801 tttattcggg cggataaggc gtttttgcct catccgccgc tctgtacaca atgcctgatg
    10861 cgacgctggc gcgtcttatc atacctacaa accacaccgc acatttcaac aatctatctt
    10921 tcatcccata ttcatcaaca tccgctatta ttgatttcca gcttaatcat cacctgatga
    10981 acaaaaataa tgactaaacc taatcatgag cttagcccgg cgctgatcgt gctgatgtct
    11041 atcgccaccg gtctggcggt agccagtaac tattacgccc agccattgct cgacaccatc
    11101 gcgcgtaact tttccctttc cgccagttcg gcaggcttta ttgttaccgc cgcgcagttg
    11161 ggctatgccg caggtctgct gtttcttgtt cccctcggtg atatgtttga acgccgccgc
    11221 ctgattgtct cgatgacctt actggcagca ggcggcatgt taatcactgc cagcagtcag
    11281 tcgctggcga tgatgatcct cggtacagca ttaaccggtt tattttcagt cgtggcacaa
    11341 attctggttc cactggcagc gacgctggct tcaccggaca aacgcggcaa agtggttggc
    11401 accattatga gcggtctgct attggggatc ttactcgcac ggaccgttgc cggattgctg
    11461 gcgaatctcg gcggctggcg caccgtcttt tgggttgctt cgatgttaat ggcactgatg
    11521 gcgctggcat tatggcgtgg tctgccacaa atgaaatcag aaacccacct caactaccca
    11581 cagttgttgg gttccgtttt cagcatgttt atcagcaata agatcctgcg cacccgcgcg
    11641 ttgctgggct gcctgacctt tgccaacttc agcattctct ggacctcaat ggcctttttg
    11701 cttgccgctc caccttttaa ctacagcgat ggtgtaattg gtctgtttgg gcttgcggga
    11761 gctgccggag cgttgggcgc tcgtccggcg ggcggttttg ccgataaggg caaatcgcac
    11821 cacaccacaa ctttcggtct gctgctgctg ctgttacttt catggctggc aatctggttt
    11881 ggtcacactt ccgtactggc gctgattatc ggcatcctgg tgctggacct caccgtgcag
    11941 ggcgtgcata tcactaacca gacggtaatt tatcgaatac accctgatgc gcgtaatcgc
    12001 ctgaccgcag gttacatgac cagctacttt attggcggtg ccgctggttc gctaatttca
    12061 gcctcagcct ggcaacatgg cagttgggct ggcgtgtgtc tggctggcgc gacgattgcc
    12121 ctggttaact tactggtctg gtggcgaggt tttcatcgtc aggaagccgc aaattaagca
    12181 atcagcaacc gttttcgtgg ctttacactt ataagggtgt taagaagccc atcagtctga
    12241 taaggttaag atattcattc agtctattta taatattaac aatcgttaag cgtacactct
    12301 atggaaagcc ctactccaca gcctgctcct ggttcggcga ccttcatgga aggatgcaaa
    12361 gacagtttac cgattgttat tagttatatt ccggtggcct ttgcgttcgg tctgaatgcg
    12421 acccgtctgg gattctctcc tctcgaaagc gtttttttct cctgcatcat ttatgcaggc
    12481 gcgagccagt tcgtcattac cgcgatgctg gcagccggga gtagtttgtg ggttgctgca
    12541 ctgaccgtca tggcaatgga tgttcgccat gttttgtatg gcccgtcact gcgtagccgt
    12601 attattcagc gtctgcaaaa atcgaaaacc gccctgtggg cgtttggcct gacggatgag
    12661 gtttttgccg ccgcaaccgc aaaactggta cgcaataatc gccgctggag cgaaaactgg
    12721 atgatcggca ttgccttcag ttcatggtca tcgtgggtat ttggtacggt aataggggca
    12781 ttctccggca gcggcttgct gcaaggttat cccgccgttg aagcagcatt aggttttatg
    12841 cttccggcac tctttatgag tttcctgctc gcctctttcc agcgcaaaca atctctttgc
    12901 gttaccgcag cgttagttgg tgcccttgca ggcgtaacgc tattttctat tcccgtcgcc
    12961 attctggcag gcattgtctg tggctgcctc actgcgttaa tccaggcatt ctggcaagga
    13021 gcgcccgatg agctatgagg ttctgctgct tgggttactg gttggcgcgg cgaattattg
    13081 cttccgctat ctgccgctgc gcctgcgtgt gggtaatgcc cgcccaacca aacgtggcgc
    13141 ggtaggtatt ttgctcgaca ccattggcat cgcctcgata tgcgctctgc tggttgtctc
    13201 taccgcacca gaagtgatgc acgatacacg ccgtttcgtg cccacactgg tcggcttcgc
    13261 ggtactgggt gccagtttct ataaaacacg cagcattatc atcccaacac tgcttagtgc
    13321 gctggcctat gggctagcct ggaaagtgat ggcgattata taacccacaa gaatcatttt
    13381 tctaaaacaa tacatttact ttatttgtca ctgtcgttac tatatcggct gaaattaatg
    13441 aggtcatacc caaatggata gttcgtttac gcccattgaa caaatgctaa aatttcgcgc
    13501 cagccgccac gaagattttc cttatcagga gatccttctg acccgtcttt gcatgcatat
    13561 gcaaagcaag ctgctggaaa accgcaataa aatgctgaag gctcagggga ttaacgagac
    13621 gttgtttatg gcgttgatta cgctggagtc tcaggaaaac cacagtattc agccttctga
    13681 attgagttgt gctcttggat catcccgtac caacgcgacg cgtattgccg atgaactgga
    13741 aaaacgcggt tggatcgaac gtcgtgaaag cgataacgat cgccgctgcc tgcatctgca
    13801 attaacggaa aaaggtcacg agtttttgcg cgaggttcta ccgccgcagc ataactgcct
    13861 gcatcaactc tggtccgcgc tcagcacaac agaaaaagac cagctcgagc aaatcacccg
    13921 caaattgctc tcccgtctcg accagatgga acaggacggt gtggttctcg aagcgatgag
    13981 ctaacgcgtc atctcgctca aaaatccaga tttataaaag aaaaaataac cggccagcat
    14041 cgtaacatgc tggccttttt ggcaagcagg tcggctcagc cgatgagtta agaagatcgt
    14101 ggagaacaat atgagcgcaa atgcggagac tcaaaccccg cagcaaccgg taaagaagag
    14161 cggcaaacgt aagcgtctgc tcctccttct caccttgctc tttataatta ttgccgtagc
    14221 gatagggatt tattggtttt tggtactgcg tcatttcgaa gaaaccgatg acgcatacgt
    14281 ggcagggaat cagattcaaa ttatgtctca ggtgtctggc agcgtgacga aagtctgggc
    14341 cgataacacc gattttgtaa aagaaggcga cgtgctggtc actctcgacc cgacagatgc
    14401 tcgccaggca tttgaaaaag ccaaaactgc actggcttcc agcgttcgcc aaacccacca
    14461 gctgatgatt aacagcaaac agttgcaggc gaatattgag gtgcaaaaaa tcgccctcgc
    14521 gaaagcacaa agcgactaca accgccgtgt gccgctgggc aatgccaacc tgattggtcg
    14581 cgaagagctg caacacaccc gcgacgccgt caccagtgcc caggcgcaac tggacgtcgc
    14641 tattcaacaa tacaatgcca atcaggcgat gattctgggg acgaaactgg aagatcagcc
    14701 tgccgtacaa caggctgcca ccgaagtacg taacgcctgg ctggcgctgg agcgtacccg
    14761 tattgtcagt ccgatgaccg gttatgtctc ccgccgcgcg gtacagcctg gggcgcaaat
    14821 tagcccaacg acgccgctga tggcggtcgt tccagccacc aatatgtggg tggatgccaa
    14881 ctttaaagag acgcagattg ccaatatgcg tatcggtcag ccggtcacca tcaccaccga
    14941 tatttacggc gatgatgtga aatacaccgg taaagtggtt ggtctggata tgggcacagg
    15001 tagcgcgttc tcactgcttc cggcgcaaaa cgccaccggt aactggatca aagtcgttca
    15061 gcgtctgcct gtgcgtattg aactggacca gaaacagctg gagcaatacc cgctgcgtat
    15121 cggtttatcc acgctggtga gtgtcaatac cactaaccgt gacggtcagg tactggcaaa
    15181 taaagtacgt tccacaccgg tcgcggtaag caccgcgcgt gaaatcagcc tggcacctgt
    15241 caataaactg atcgacgata tcgtaaaagc taacgctggc taatccagag gtgcgtgtga
    15301 tgcaacagca aaaaccgctg gaaggcgcac aactggtcat tatgacgatt gcgctgtcac
    15361 tggcgacatt catgcaggtg ctggactcca ccattgctaa cgtggcgatc cccactatcg
    15421 ccgggaatct gggctcatca ctcagtcagg gaacgtgggt gatcacttct ttcggggtgg
    15481 cgaatgccat ctcgatcccg cttaccggct ggctggcaaa gcgcgtcggg gaagtgaaat
    15541 tgttcctctg gtcaaccatc gcctttgcta ttgcgtcgtg ggcgtgtggt gtctccagca
    15601 gcctgaatat gctgatcttc ttccgcgtga ttcaggggat tgtcgccggg ccgctgatcc
    15661 cgctttcaca aagtctattg ctgaataact acccgccagc caaacgctcg atcgcgctgg
    15721 cgttgtggtc gatgacggtg attgttgcgc caatttgcgg cccgatcctc ggcggttata
    15781 tcagtgataa ttaccactgg ggctggatat tcttcatcaa cgtgccgatt ggcgtggcgg
    15841 tggtgttgat gacactgcaa actctgcgcg gacgtgaaac ccgcaccgaa cggcggcgga
    15901 ttgatgccgt ggggctggca ctgctggtta ttggtatcgg cagcctgcag attatgctcg
    15961 accgcggtaa agagctggac tggttctcat cacaggaaat tatcatcctc accgtggtgg
    16021 cggtggtggc tatctgcttc ctgattgtct gggagctgac cgacgataac ccgatagtcg
    16081 atctgtcgtt gtttaagtcg cgcaacttca ccatcggctg cttgtgtatc agcctcgcgt
    16141 atatgctcta cttcggcgcg attgttctgc tgccgcagtt gttgcaggag gtctacggtt
    16201 acacggcgac gtgggcaggg ctggcctctg cgccggtagg gattattccg gtgatcctgt
    16261 cgccgattat cggccgcttt gcgcataaac tggatatgcg ccgactggta accttcagct
    16321 ttattatgta tgccgtctgc ttctactggc gtgcctatac ctttgaaccg ggtatggatt
    16381 ttggcgcgtc ggcctggcca cagtttatcc agggttttgc ggtggcctgc ttctttatgc
    16441 cgctgaccac cattacgctg tccggtttgc caccggaacg actggcggcg gcatcgagcc
    16501 tctccaactt tacgcgaacg ctggcggggt ctatcggcac gtcgataacc acgaccatgt
    16561 ggaccaaccg cgagtcactg caccatgcac agttgactga gtcggtaaac ccgttcaacc
    16621 cgaatgctca ggcgatgtac agtcaactgg aagggcttgg tatgacgcaa cagcaggcat
    16681 caggctggat tgcccagcag ataaccaatc aggggctgat tatttccgcc aatgagatct
    16741 tctggatgtc agccgggata ttcctcgtcc tgctggggct ggtgtggttt gctaaaccgc
    16801 catttggcgc aggtggcggc ggaggtggtg cgcactaagg ttgccaaagg gagctgcggc
    16861 tcccttttta tttgctcatt ctgcaaacgg ttgttttata cactcattcg gtgtatcttt
    16921 tcgccgcccc aaactcttcc attcaccatt aaccggttct gagcattcgc tcaggttgtt
    16981 aattctcttt ttccttcgcc cttgctttcg cgggtgatac tcacattttt attttaaccc
    17041 cataactcgc accctcgcgg gcgtacattt attacacaca aaataatgga ttattatgaa
    17101 tacccaacga aaatacggga gaacctggca ttaccccttc tctccaggca ccaccagcga
    17161 tgaccgcatt aacaccgatt actggcagga tttaaaggcc atcacgcaat tggtgcacac
    17221 agaaaaactc gatggcgaaa ataactgcct taaccgctac ggcgtgtttg cccgctccca
    17281 tgccacgcct acgcagtcgg catggaccta taaaattcgc caacgctggc aactgctgaa
    17341 aaacgatctc ggcgacctgg agttgtttgg tgaaaatctc tacgccgttc attccattga
    17401 ataccgcgcg ctggaacagg acttttacct gtttgccgtt cgctgccagg atatgtggct
    17461 gagctgggaa gaggtgcagt tttacgccgc tctgtttgat ttcccctgcg ttcccgaaat
    17521 ctccggcccg cagccgggga atgatgaaaa aagctggcaa cgtgattttc tggcgctgac
    17581 caatgcgcgc ggcacattcg atccctggga tacgcaaacc tgtcaaccct gtacgctgga
    17641 aggcattgtc agccgaaatc atgatgcgtt ttctgtcgct gacttttcgc ataacgtctt
    17701 caagtatgtg cgcaaaaatc atgtcaaaac cactgtgcac tggaaacgcc actggcaacg
    17761 ggcgcggatg gcccatgagt tcgtttatgg agaacagtca tgatttggca acttaccgac
    17821 gataaacgct ggtcggcgct acgtcagcgt tttagctggg ttgaggagat gcatcacacg
    17881 ccacaagacc ctgagcatca tggtgaaggc gacgtcggcg tacataccga aatggtgctt
    17941 aacgccctca ttactctgcc agaattccag caactccccg ctcaacagca ggaagtttta
    18001 tgggcggcag cgttattgca tgatgttgaa aagcgcagca ctaccgtgca ggaaaacgga
    18061 cgtattcagt ctcctggtca tgctcgtcgg ggcgaactga cggcccgaca aattttatgg
    18121 cgcgatatcc caacgccgtt cgtgctgcgc gagcaaattg ttgcactggt gcgtctgcat
    18181 aggttaccgc tatggttact ggagcgcccg gaaccggagc gtttgttgct cgcggctgca
    18241 atgcgcatcg acacgcgtct gctcgctttg cttgctcgtg ccgatctgct tggtcgccaa
    18301 agcccggatc agcaatcaat gctggaacgc atcgatctgt tcgagctgtt ttgccatgag
    18361 caacaatgct ggggaaaaat gcgcccgttc gtttctgatt ccgcacgctg gcattacctg
    18421 acccatgagc aaagttctcc tgattttgtt ccctgggagg ctgaaccttt tgaggttatt
    18481 ttgctgtgtg gcttaccggg tatgggcaaa gatcgctata tcaatgaaca gtgccaggga
    18541 atggacgtta tcagccttga tgatatgcgg cggcgaatca atgccagtcc ggacgacaaa
    18601 accgccaccg ggcgtattgt gcaacaggca aaagaagagg cacgcgtttt tttacgccag
    18661 aaaaagccgt tcatctggaa tgcaacgaac attacccgcc agctgcgtag ccagctcatc
    18721 agcctgttta cggcttacgg cgcacgagta aaaatcgttt atcttgaagt gccatgggcg
    18781 caatggaagc agcaaaatgc cagacgcgag tatgcagttc ctgaagcggt cgtgatgcgg
    18841 atggcctcaa ggctggaagt tcctcaacct gatgaagcac atagcgtgga atatcggatg
    18901 actgacaggt aacaaaagcc gggcggtgtt ttcaccagcc cggcctcttc tgcttaaact
    18961 gactaaatgt gcagttcctg caacttctct ttcggcagcg ccagttcttc gttgctgttg
    19021 atgcgcacgt cacgttccag aatgttacgc gcaatatcct gcgcttcctg caacgagtgc
    19081 atctggtaag tgccacactg gtagacgttc agttccggga tctgattctg atcctgcact
    19141 ttcagcacgt cttccattgc cgctttccag gcatcagcga cacgctgctc atctggcgta
    19201 ccaatcagac tcatataaaa accggtgcgg cagcccattg gcgagatatc gataatctct
    19261 acgccattac cgttaagatg gttacgcata aaaccagcaa acaggtgctc cagggtatgg
    19321 atccctcttt ctggcatcac ttctttgttc ggcacgcaga agcgcagatc gaacacggtg
    19381 attgcgtcgc catgcggggt gttcattgtt tttgccaccc gaactgcagg cgcttccatc
    19441 cgagtatgat cgactgtgaa gctatctaac aacggcattt agccacctcc ggtaattttt
    19501 ttaaaaattt tctgaactct ttgttcccag gcgagtctga gtatatgaaa gacgcgcatt
    19561 tgttatcatc atccctgaat tcagagatga aattttggcc actcacgagt ggcctttttc
    19621 ttttctgtca ggcgtgcttt tccagccaca ccgcaaacgg ttcggtatca gcggcttcca
    19681 tttcctgctg acggcgttca gacgcctcgc gctcggctac aaaatcctct tcgcgcagaa
    19741 tttccagcgg ctcttcacgc agcagattac ggtacgcttc agcaaacgct ttgcctgttc
    19801 cgccaatacc agtatcaatc atagacctta agatacgggc agagaaagtc agatcaggat
    19861 tatcgaagca ggcgaccagt tcatcacaca ctttctgata cgcttcgccg ccgttaatgc
    19921 tatccagcgt ttgcgcgacg cgtttcagat cgcggaacag atctttaccc acctgcggta
    19981 acgggaactg cgcagtttcg cagccgatac ccagcgtcag gcccggtttg cgaccttcga
    20041 ggatcacccg gttccagtta acgcgtgtac aggcaagttc gctactgctc atttccggtg
    20101 cgtcagccag cgcacaccag accataaaca ggtcgaggaa tcgcacctgt tgttcatcta
    20161 caccaatcgg cgagaacggg ttgatgtcca gcgagcggac ttcaatatat tcaatgccgc
    20221 cacgtaacag cgcatcagaa ggcgactcgc cgctgcgggt aacgcgtttt ggacgaatcg
    20281 gcgcgtacag ttcgttttca atctgcagca cgttgctgtt gatttgcagt cgcttaccgt
    20341 ctttctcaat accaatcttc gcgtactctt ccgatggcgt tttgattgcc tgtttaaggc
    20401 ccgccacgta ctcgtagaga tcattgaagg tgataccaag attgctttgc gatttattgg
    20461 tatagccgag atcgctcaaa cgaagagagg tcgcatacgg caggtaatac ataccgcact
    20521 cggttttctc aaacggcagc gacgttggtt ttccttgcaa gaaagaagaa caaatcgctg
    20581 gagacgcacc aaacagataa ggaatgaccc aaccgaagcg atagtagtta cggataacac
    20641 ggaaataacc cgcagatatt ttctctttgg catcagcgcc cgagatatca ccgcacttcg
    20701 cttgccagaa tgccattggc aaagagaaat tatagtgcac gccggaaatg gtttgcatca
    20761 gcgcgccgta gcgatttttc agcccttcac ggtacagcgt tttaaagcga ccggtgttag
    20821 aagtgccgta ctgtgccagt tcgatgtcct gaccttctgc gatatagcat ggcatactta
    20881 acggccacat ccgctcatcg cccatattgc gcgccgtata gcgatgcaga tcgcgcataa
    20941 aggtcagcat atgttcaata tcaccatcca ctggtgtaat aaattccagc aatgcttccg
    21001 caaaatcggt agtaatccat ttatgggtca gtgcagaacc taatgcttca ggatgacctg
    21061 ttgttgccag tgtgccatca gcattaacac gcaaagtttc gcgctccagc ccacgctgta
    21121 tcccctttaa cgcctgagga tgtttttcca gccaggccag cgcctgtgat acgtccggga
    21181 tcaaattgac ctcccgcctg tcaaaatcgt tttaattagc ataactgtaa tggtgaccat
    21241 atgtgcaggc ctacaattag tgccaccaca tcatgccctg aacggtagct gctgcaactg
    21301 caacatagcg taacgcttta ccaaggcaca aaaaaaagat taccggtccc cacgagatgc
    21361 gcatccatcc cgctaacaga cacagtaaat cgccaaccac gggcatccag cttaataata
    21421 gcgtgactgc accatagcgt ttcagccagc cggtagcttt ctcttgccag cgcgatgttt
    21481 tacgcaatgg aaagaaacgc ccaaggataa cgttagttaa ccctccaagg ctattaccca
    21541 ttgttgctgt taaaactaaa acccagggat gactcactcc ggaaagcaac attgccacca
    21601 gcacgacttc ggagttgccg ggtaatagtg tagcgctgag aaaactactg gcaaacaacg
    21661 agaaaagcga taacgcttca ctcacagcaa gcgaacatcc acggcgtcca tgcctgctgc
    21721 acgggccgcc tgaataccga aatcggcatc ttcaaagacc acacactgcg tcggttgcac
    21781 gcccatacgc tgcgcgcaca acaaaaatgt gtctggcgcg ggtttatggt gtttgacgtg
    21841 atcggcagcg acgacggcgt caaaataacg gcgtaatccc aggtgtgcca gcaatgcctc
    21901 agcgatggcg ctttcactcc ccgttcctac agccattggg cgacgaccat gccagctttt
    21961 caccacttca acaagaggaa gcggctcgac actatccagc agcatacttc ttactgcttc
    22021 tgttttttca cgcgctaacg catgcgggtc gagatcggcc tgattcagct caataatcgc
    22081 ctgagcaata cgccaggtgg gcgagccatt aagcgcaatc atcgcctgaa catcgtactg
    22141 aagaccgtag taccctaata cttcgcgcca cgctttacgg tgcgtaggct cggtatccag
    22201 gatggtgcca tccatatcaa aaattaaacc tgcataacgc tcgtacatgg tcttctcgca
    22261 agtagaaaaa tatgacgcta ctttagcgta attgcttgat tttgtcgctg atggagaagg
    22321 agaagaaaat tcaggaaatg gataaagtag ttatttagaa atgagatatt tttgaaggaa
    22381 attttttgga gaatatggtg catccgggag gatgactcac tggcgttcgc ccttcgggcc
    22441 gctgctggcg caacgttatc ctcccaggtg cctgcggtta ataccgctac ctcttcgata
    22501 ccttcattgc tgaaggttac ggagaatatg gtgcatccgg gaggattcga acctccgacc
    22561 gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcatcgg aaaaatttat
    22621 tttactgctg atattgatat cgctactaaa gccatatcaa gtaagagatg gtgcatccgg
    22681 gaggattcga acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg
    22741 gatgcatcgg gaaat
//
LOCUS       DAZGJT010000078        22063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZGJT010000078 DAZGJT010000000
VERSION     DAZGJT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.078"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(32..820)
                     /gene="phoH"
                     /locus_tag="ACHKFK_003289"
     CDS             complement(32..820)
                     /gene="phoH"
                     /locus_tag="ACHKFK_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFO9053558.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVRSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1451..2722)
                     /gene="efeB"
                     /locus_tag="ACHKFK_003290"
     CDS             complement(1451..2722)
                     /gene="efeB"
                     /locus_tag="ACHKFK_003290"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO9053559.1"
                     /translation="MQYEDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGVAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNVKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMLHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMELLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(2728..3855)
                     /gene="efeO"
                     /locus_tag="ACHKFK_003291"
     CDS             complement(2728..3855)
                     /gene="efeO"
                     /locus_tag="ACHKFK_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO9053560.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADIPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDKYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFENYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3913..4743)
                     /gene="efeU"
                     /locus_tag="ACHKFK_003292"
     CDS             complement(3913..4743)
                     /gene="efeU"
                     /locus_tag="ACHKFK_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO9053561.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFVLPPRAGATASRSV
                     "
     gene            complement(4963..6471)
                     /gene="putP"
                     /locus_tag="ACHKFK_003293"
     CDS             complement(4963..6471)
                     /gene="putP"
                     /locus_tag="ACHKFK_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO9053562.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEG"
     gene            complement(6630..6839)
                     /locus_tag="ACHKFK_003294"
     CDS             complement(6630..6839)
                     /locus_tag="ACHKFK_003294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053563.1"
                     /translation="MMLQLCATVLNVTCVASLKMNEMLINEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            6894..10856
                     /gene="putA"
                     /locus_tag="ACHKFK_003295"
     CDS             6894..10856
                     /gene="putA"
                     /locus_tag="ACHKFK_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9053564.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQSLPMLEQSVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGAQLTGDDRVRGMMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRQVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPALIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(10896..11534)
                     /gene="rutR"
                     /locus_tag="ACHKFK_003296"
     CDS             complement(10896..11534)
                     /gene="rutR"
                     /locus_tag="ACHKFK_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO9053565.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            11822..12913
                     /gene="rutA"
                     /locus_tag="ACHKFK_003297"
     CDS             11822..12913
                     /gene="rutA"
                     /locus_tag="ACHKFK_003297"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO9053566.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGSGKS
                     DFKGDFFTMDDCRVSPQPSVPMKVICAGQSDAGMAFSARYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVITDETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVANVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMLCRAHLPALTQEVA"
     gene            12913..13605
                     /gene="rutB"
                     /locus_tag="ACHKFK_003298"
     CDS             12913..13605
                     /gene="rutB"
                     /locus_tag="ACHKFK_003298"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO9053567.1"
                     /translation="MTTLTARPEAITFDPQQTALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARTAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSSTSFARIA"
     gene            13617..14003
                     /gene="rutC"
                     /locus_tag="ACHKFK_003299"
     CDS             13617..14003
                     /gene="rutC"
                     /locus_tag="ACHKFK_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO9053568.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            14011..14811
                     /gene="rutD"
                     /locus_tag="ACHKFK_003300"
     CDS             14011..14811
                     /gene="rutD"
                     /locus_tag="ACHKFK_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO9053569.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLDQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTVLVSVNGWLRINAHTRRCFQVREQLLHSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSRHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            14821..15411
                     /gene="rutE"
                     /locus_tag="ACHKFK_003301"
     CDS             14821..15411
                     /gene="rutE"
                     /locus_tag="ACHKFK_003301"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO9053570.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            15422..15916
                     /gene="rutF"
                     /locus_tag="ACHKFK_003302"
     CDS             15422..15916
                     /gene="rutF"
                     /locus_tag="ACHKFK_003302"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO9053571.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPVC"
     gene            15937..17265
                     /gene="rutG"
                     /locus_tag="ACHKFK_003303"
     CDS             15937..17265
                     /gene="rutG"
                     /locus_tag="ACHKFK_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO9053572.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKNVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNSVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(17348..17521)
                     /gene="ymdF"
                     /locus_tag="ACHKFK_003304"
     CDS             complement(17348..17521)
                     /gene="ymdF"
                     /locus_tag="ACHKFK_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9053573.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            18337..18774
                     /locus_tag="ACHKFK_003305"
     CDS             18337..18774
                     /locus_tag="ACHKFK_003305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO9053574.1"
                     /translation="MKNLLNNFPDNCIENILPKDDFGYGRLLIGSKGKRIKTGAHRLI
                     YRIFIGEIPAGMVVRHSCDNPACCNPKHLLIGTILDNNADRQKRKRQARHEKHGRAKL
                     TKEQVLFIRANEMSIPNSVLADKYRVSNSLIWQIKNYRIWNEL"
     gene            19055..19684
                     /locus_tag="ACHKFK_003306"
     CDS             19055..19684
                     /locus_tag="ACHKFK_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFO9053575.1"
                     /translation="MTTQLIPVFEGTISNEPTLLVNARDLHGFLEVDKDFSNWIRARL
                     NEYGFVENLDYILFSPNLAKTLGRRRKDYHLTLDTAKELAMVERNEKGRQIRRYFIEC
                     EKKLRNIQPVQAESQPQFTAEEIILLCYMQLWMEKAQDLSKQLYPIMKELNSSYTNKL
                     YDIAFETIYMVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLRARQFEF"
     gene            19881..21107
                     /locus_tag="ACHKFK_003307"
     CDS             19881..21107
                     /locus_tag="ACHKFK_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9053576.1"
                     /translation="MPVTITKIRTGEDVLSAAIHRIEWLFETFSSVCLSFSGGKDSTV
                     LFHLVAEVAYRKKRRFSVLFIDWEAQYQCTIDHIQKMRMMYQDVTDTFYWVALPLTTV
                     NGVSQFQPEWICWEPGVEWVRQPPEDAITDMAYFPFYRYAMTFEEFVPAFSSWFASNQ
                     CSVAILTGVRADESLNRFMGLVSQRKLRYADDKPWTTASPEGFYYTMYPLYDWKTNDI
                     WVYNAKTCAIYNPLYDLMYRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMCE
                     RVSGAASGALYANESGAYFALRKRMSKPSHHTWRSYAFFLLDVMPERTAEHYRNKIAV
                     YLRWYQNRGFPDDIPDEQENDLGCRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHYE
                     RYLQRMNERRKEWGIL"
     gene            21092..21736
                     /locus_tag="ACHKFK_003308"
     CDS             21092..21736
                     /locus_tag="ACHKFK_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000528463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO9053577.1"
                     /translation="MGNSVTPEVEVLSKMIRQYFSQEQSEEKTIQALNHLRCVLHEIS
                     PFAQEPVDCVLWVKADEIVANDYNPNVMAPSEKKLLKQSLEKDGFTQPIVVSEETSHY
                     LVVDGFHRQLLGRRTVTGKRLKGWLPVTCINPDRKGQTSRIAATIRHNRARGKHQITS
                     MSDIVRDLSRLGWTDERIGTELGMDQDEVLRLKQISGLTELFQQADFSPAWTVT"
     gene            complement(21825..>22063)
                     /locus_tag="ACHKFK_003309"
     CDS             complement(21825..>22063)
                     /locus_tag="ACHKFK_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053578.1"
                     /translation="EIESMDIIFGQYGGWDAFRLCDETHRICGEWRDPHGSSIPISLK
                     DIFIALGKLPEAATVMANSINAQNNLDILLGDLR"
BASE COUNT         5382 a         5813 c         5579 g         5289 t
ORIGIN      
        1 ggttcgcccg ggctctgcac cgataataca cttaactgta tgcatgtagc gtacgttggc
       61 aaagtgccga acgtacgcaa tcctctttac cgaatcggac gatcccgacc atttcatcct
      121 cttcgaaacg ttccagcgcg tcacttaatc cggagcgcac gccgcgaggc aaatcgcatt
      181 gcgtgatatc accgttgacg ataaccgtca cgttctcccc gaggcgggtt aaaaacattt
      241 tcatttgcgc ggcagtcaca ttctgcgcct cgtcaagaat gacgactgca ttttcaaagg
      301 tacgtccacg catataggcg aacggcgcaa tttccacctt ccctatttcc ggtcgcaggc
      361 agtactgcat aaaggaagcc cctaagcgac ggaccagcac gtcgtagacc gggcgaaaat
      421 agggagcaaa cttttctgcg atatcaccag gtaagaaacc aagatcttca tcggcttgca
      481 gaactggacg ggtaacgata atcctgtcga catccttatg tatcagggcc tctgccgctt
      541 ttgctgcgct gatccaggtt tttccgcacc cggcttcgcc cgtggcaaat atcagctgct
      601 tactctcaat agccttcaga tagtgcaatt gcgcttcatt tcgcgcgagg atgggcgagg
      661 tatcgcgact gtcgcgggcc ataccaatgg cttctacgcc gctcatctgc acaagcgagg
      721 tgaccgattc ttcttcacgc tgcttatggc tgcgcgaatc ccgtctcagc acacgttttg
      781 cctcgcgacg agctttgatc actgcttttt gtcttcccat ggagagcacc ttgagttgtt
      841 tgtattcatc acacgcgccg ttggcagcgc gattatgcgc acgaacatca gagggttggc
      901 ttccttgtaa gccatagttt gcttttggat aaaatgccga aaaacggcta cgcacaccgt
      961 ttgcggcgtc ggtaacacga gtaaagagag gaagtgagaa aaatacagag tgacgaagtt
     1021 gctaccggag gagaaatctc cgcgagtggt ggggccttgc ttaacaaaac attgtccaga
     1081 acaggacgtt accatccgcg atctccatag tgactgacta tcactgccgg gaacttccgc
     1141 tgctacttaa taagtacaac agatctcgca ttcattgcaa caatatattc acttatattt
     1201 aactataaaa caccatttca gtgacattag tttctactgg aaagatgaca gagtgatgac
     1261 agtgatgaaa aaagctgtgt gctttcagca ggattcgcat caggcatcag cgcacgaacg
     1321 ccggatgcgg cgtgaacgcc tgatccagcc taccaaaccc gcaccgattg taggcatgat
     1381 aagacgcgca agcgtcgcat cagcgcacga acgcctggtg cggcgtaaac gccttatccg
     1441 cctaaaaaca ttaaacccgc aataatgcgc ttccgagata atcgttcgcg tccttcacac
     1501 ccggcagcgc aaagaaatag ccgccaccga taggtttgac atattcctcc agcgcctcgc
     1561 cattcaacct tttttgtact gtcaggaagc ccttttccag atcgtgttgg tagcagacaa
     1621 acagtagctc catatcgagt tgcccggaat tggtgacgcc caacgaataa ctgtagccac
     1681 gacgcagcat caggctggac tcgctctccg ccgtgcgggg attcgccagc cggatatggc
     1741 tgtccagcgc aatcaccttc ccttccggat cgctagcgta atcgggaaca tcatgctcat
     1801 gcagcatgcc cagcggcgca ccggtttgct tatcacgacc aaatatcgtc tgctgctctt
     1861 tcagcggcgt tctgtcccaa aattccactc gaaactgaat caagcgcact gcctgatagc
     1921 tgccaccgat tgtccacgca ggctcctgct gatctgccgt cacccacacc actttttgca
     1981 tcaacttcac attctggcta tcgggattgg cggtgccgtc tttaaagccc agcaaattta
     2041 tcggcgtctc tttgccttta ctacgcgctg cgtggtcgga aataaaccct tcccgcttcc
     2101 agcgcacgct tagcaaatcc ggcgtgtgtt tgatgatatc gcgcagcgca tggatcaccg
     2161 tgtcctgggt gttggcgcaa atttgtagca acacatcacc atgacataac gccgcatcca
     2221 gcgagtcgtt agggaaacgc gtcatcttct gcaacttttt tggcatctgt ggcgcaaggc
     2281 caaagcgctc atcaaacaat gagtgaccca ccgataacgt gatggtgaga ttatcgggcg
     2341 caatgtagcc accaagaatg ccggaatcga gtggtggcag gcgcggattt ggcgtttctg
     2401 gtgcaactcc gccctgagtc agaaaagcaa aacgctgagt caacaagcga aacaaccgtt
     2461 caagatcggc tttatcgctg gcaagcacat caaacgccac cagcatcatt gcagcctgtt
     2521 gtggtgtcag gatccctgcc tgatgctcac cataaaacgg ctgtttctca ttgcgtgcat
     2581 ccggtgaaag cgtacccggc gcactttgcg ttttttgtgc atgagcgacc ggacaacttc
     2641 ccgccagcgc cagcgcacca atccctttca gtaaacgtcg gcgtgacggt tcattcacgc
     2701 cgttttcatc ttcatactgc ataacgctta gtccagcccc agcacaccgc gaagttgcgc
     2761 cagatcttcc gccagcgcag taatcggtcc tttcagtgca ttccggtcag catcggtcag
     2821 tttgtcgtaa ttttcaaaac cgtctttagt acggtatttc gccagaatgg tatcgacctt
     2881 tttgaagttg gcatcgactt ttgccaacag ttccggattc gctttttgta gttgtggacg
     2941 cagcagatcg acaattttct gcgagccttc aacgttagcc tggaaatccc acagatcggt
     3001 gtggctgtag cgatcttctt caccgctgat tttgctggct gccacttcct caatcagtcc
     3061 ggctgcaccg ccgaccactt ttgaaggagg gaaagccagt tcactgatgc gtttttgcaa
     3121 atcgaccaca tcggtataaa gctggtcagc atacttatcc attcctttgg tggtgttatc
     3181 gccaaacaat gctttttcca gacggtggaa accggtgaat ttcggatcgg cggctttttg
     3241 ctcgtaatca tcttcacggg cgtcaatgct gccatccaga tcggagaaca gttcagcaat
     3301 cggttcaatg cgctcatagt gctggcgcgt cggcgcatac agtgctttcg ctttttcgat
     3361 atcgcctgct ttaatagcgt cggtaaaggc tttggtgtcg gtcaccagtt gcgtggtttc
     3421 cgccatgaca tacgctttat atgcagtaat tgcaccacca agacttaaca gcgcatcact
     3481 ttgcgccgca tccgccgttg cttcaccttt gacgatcaac ttccctttcg ggttagtcag
     3541 cagaccgcag gtcatatcgt attcgccagg ctgtaaattc gccgtcattt tctggctaaa
     3601 accaggggcg atattttccc gctcttccac caccatcacg cctttgagga tctcccactc
     3661 cagcgccttc tggctgtggt tctgaataat gaactgtgtt ttcccggcgt taaccgtaat
     3721 ggtcatcggc tcgcactgct tatccgtcac ggtcactttg acttgcggaa tatcagcggc
     3781 gttagccata aaagcagaag agaacagcgc agccacgctc aactgcaatg cgttacggcg
     3841 gaagttaatg gtcatgacga gactatccct ttaaagtatg ttgtaactaa gcaagagttt
     3901 gcgtcgtatt tgctacacgg agcgggacgc agtcgcccct gcgcgtggtg gcagaacaaa
     3961 tgccaccagc gccgggatga gataaataaa ccagacggcg acttcgctga cgctcggtgc
     4021 ttcctgataa ccaaaaatcc cttccatcag tgtgccaaac agcgagtgag ttgagagcac
     4081 cgcgctcata tcgaaggcga tttcctgaaa gtggttccac aatccggctt catgaaatgc
     4141 gcgaatggca ccagctgcca gccctgcggc gacgaagaga ataaacagac tggtccattt
     4201 aaaaaatgca ccaagattga ggcgaatacc gccccagtag agcaggaagc caagcaccac
     4261 ggctgtagca agaccaagca ttgcacccag cggcggccag atcccgacat cttgttgaaa
     4321 tgccgccagc aggaaaaaga ccgactccag cccttccctt gcaacggcaa aaaagaccat
     4381 catcaccagc gcccagccat gatgatttcc acgctgcaat gcgctatcga ccgcctgctc
     4441 cagttgtact ttgacgttgc gcgacacttt gcgcatccag aaaaccatcc aggtaaggat
     4501 cactacggcg atcaccgcca caatgccttc aaacagttcc tgttcttttt gcggaaattc
     4561 gccggtggtt tcgttaatga agatacccaa gcccaggcac aacgcagcgg caagcaacac
     4621 gccaatccac attacaccaa tccatcggcc tcgctgggta cgcttaagat agctggcaat
     4681 caaactgaca atcagcgcgg cttcaagtcc ttcgcgcaac ataatgagaa acggaacaaa
     4741 catgccaccc acccttaaac gtttttctga gtcaatctat gcaaagaaaa gtaaatcgca
     4801 gtgatagcga ttatcattac gatgaagaaa aatacaagtg gaatgtagtg ataatgatga
     4861 gaagatgtaa aaatattttg agaaaaaacg cagagggcaa tcactgattg ccctctgcct
     4921 gcttcctgtt atgacgtgaa atttccggta aaacgccttc ccttaccctt cctgcaaccg
     4981 tgacggcgga gccgaatgat agtgcgcatc ggcctcggca aagcgttttt gcatcgccgc
     5041 tgacggcgct ttacccagca aactaaacac tacaatcccg atactgccga agataaagcc
     5101 cggaataatt tcgtacagac ccagccagcc gaactgtttc cagacgataa ccgtcagcgc
     5161 accgatgatc atccccgcca gcgcaccgtt gcgcgtcatg cgtgaccaca tcaccgagaa
     5221 cagcaccacc ggaccaaacg ctgcgccaaa gcctgcccac gcgtagctca ctaagcccag
     5281 cacgcggttt tccgggtttg ccgccagcgc aatcgccacc agcgccacca ccagcaccat
     5341 cactcgccct acccacacca gctctttctg gctggcctgt ttacgcagaa acgctttgta
     5401 taaatcttcg gtaatcgcac tggaacatac cagcagctgg caacttaacg ttgacattac
     5461 cgccgccaga atcgccgaca gcaaaatccc ggcaatccac gggttaaaca gaatttgtgc
     5521 cagttcaata aacacgcgct cggcgttctg gtttaccgca cctgctaccg ccgggtgctc
     5581 gttaaagtaa gcgataccaa agaagccgac agccaccgcc cctgccaggc agaggatcat
     5641 ccaggtcata ctaatacgac gcgcatggac aatgctgtgg tgagaatctg ccgccataaa
     5701 acgcgccagg atgtgcggct gcccgaagta acccagcccc caacccatca gcgagataat
     5761 ggcgacaaaa ttcaaccctt tgagcatatc cacgttttca atgctctttt gtttgattac
     5821 ttccagcgag tcaccaaagc caccgacact gataatgacg ataactggcg tcaggatcag
     5881 ggcaaaaatc atcaggctgg cctgtacagt gtcagtccag ctcaccgcga ggaaaccgcc
     5941 aataaaggtg taaaggatcg tcgccgcagc ccccgcccac agagccgttt cgtagctcat
     6001 gccaaaggta ctttcaaaca gacgcgcgcc tgccacaatg cctgaagcgc aataaatggt
     6061 gaagaacagc aaaataacca gcgcggagat aatgcgcaaa atgcggcttt tatcttcaaa
     6121 gcgcccggtg aaataatccg gcaatgttaa ggcgttattg ttatattcgg tatgcacacg
     6181 caaccgcccg gccaccagtt tccagttgat ccacgcgcct aatgtcagac caatggcgat
     6241 ccagctttcg gaaatcccgg aaagaaaaac agcgcctggc aaccccatta acagccagcc
     6301 gctcatatcc gacgcacccg ccgataatgc cgtcacgaat ggcccaagac tacgaccgcc
     6361 cagaatatag tcgtcgaagt ttttcgttga tcgccaggcg ataaacccaa tcaatatcat
     6421 gccaaagata tagacacaaa atgtcaccaa catcggtgtg ctaatagcca tctaaagtct
     6481 ccaaaaaatt attatcggca atgtcgaaac ttgccgttat atctgccacc ggaacggggt
     6541 aacagagttt atgttttacc agggcgaccg tatcctgccg gaagcgctgg ttattcacaa
     6601 tcgatttaac acaccattta cattaaattt tagtgctcag cgacactatt tttcatcagg
     6661 ttgcactctc tcacattttt tgcggttgca cctttcaaaa atgttaactg ccgcagagaa
     6721 aaagtctgag ttattttttt aatccctgtc atatcgattt cgtttattaa catttcattc
     6781 atttttaagc ttgctacgca ggtcacattt aacacggttg cacaaagttg caacatcatg
     6841 gatatttcac gataacgtta agttgcacct ttcagaacaa caggagtaat ggcatgggaa
     6901 ccaccaccat gggggttaag ctggacgacg cgacgcgtga gcgtattaag tctgccgcga
     6961 cacgtatcga tcgcacacca cactggttaa ttaaacaggc gattttttct tatcttgaac
     7021 aactggaaaa cagcgatact ctcccggagc taccagcgct gctttctggc gcggccaatg
     7081 agagcgatga agcaccgact ccggcagagg aaccacacca gcctttcctc gactttgccg
     7141 agcaaatatt gccccagtcg gtttcccgcg ccgccatcac cgccgcctat cgccgcccgg
     7201 aaaccgaagc ggtttctatg ctgctggaac aagctcgcct gccgcagcca gtagctgaac
     7261 aggcgcacaa actggcgtat caactggccg ataaactgcg taatcaaaaa aacgccagtg
     7321 gtcgcgcagg tatggttcag gggttattgc aggagttttc gttgtcatcg caggaaggcg
     7381 tggcgctgat gtgtctggcg gaagcgttgt tgcgtattcc cgacaaagcc acccgtgacg
     7441 cgttaattcg cgacaaaatc agtaacggta actggcagtc acacattggt cgtagcccgt
     7501 ccctgtttgt taatgccgct acctgggggc tgctgtttac cggcaaactg gtttccaccc
     7561 ataacgaagc cagcctctcc cgctcgctga accgcattat cggtaagagc ggtgaaccgc
     7621 tgatccgcaa aggtgtggat atggcgatgc gcctgatggg cgagcagttc gtcacgggcg
     7681 aaaccatcgc ggaagcgtta gccaatgccc gcaaactgga agagaaaggt ttccgttact
     7741 cttacgatat gctgggcgaa gctgcactga ctgccgcaga cgcacaggcg tatatggttt
     7801 cctatcagca ggcgattcac gccattggta aagcgtctaa cggtcgtggt atctatgaag
     7861 gtccgggcat ttcaatcaag ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg
     7921 accgggtaat ggaagagctt tacccgcgtc tgaaatcact caccctgctg gcgcgtcagt
     7981 acgatattgg tattaacatc gatgccgaag aggccgatcg tctggagatc tccctcgatc
     8041 tgctggaaaa actctgtttc gagccggaac tggcaggctg gaacggcatc ggttttgtta
     8101 ttcaggctta tcaaaaacgc tgcccgttgg tgatcgatta cctgattgat ctcgccaccc
     8161 gcagccgtcg ccgtctgatg attcgcctgg tgaaaggcgc gtactgggat agtgaaatca
     8221 agcgtgcaca gatggacggc cttgaaggtt atccggttta tacccgcaag gtgtataccg
     8281 acgtttctta tcttgcctgt gcgaaaaaac tgctggcggt gccgaattta atctacccgc
     8341 agttcgcgac gcacaacgcc catacgctgg cggcaattta tcaactggcg ggtcaaaact
     8401 actacccggg tcagtacgag ttccagtgcc tgcatggtat gggcgaacca ctgtatgagc
     8461 aggtcaccgg gaaagttgcc gacggcaaac ttaaccgtcc gtgtcgtatt tatgctccgg
     8521 tcggtacaca tgaaacgctg ctggcgtatc tggtgcgccg cctgctggaa aacggtgcta
     8581 acacctcgtt tgttaaccgt attgccgaca cctctttgcc actggatgaa ctggtcgccg
     8641 atccagtcac tgctgtagaa aaactggcgc aacaggaagg gcaaactgga ttaccgcatc
     8701 cgaaaattcc cctgccgcgc gatctttacg gtcacgggcg cgacaactcg gcagggctgg
     8761 atctcgctaa cgaacaccgt ctggcctcgc tctcttcagc cctgctcaat agtgcactgc
     8821 aaaaatggca gtccttgcca atgctggaac aatcggtcgc ggctggtgag atgtcgcccg
     8881 ttattaaccc tgcggaaccg aaagatattg tgggctatgt gcgtgaagcc acgccgcgtg
     8941 aagtagaaca ggcgctggaa agtgcggtta ataacgcgcc aatctggttt gccacgcctc
     9001 cggctgaacg cgcggcaatt ttgcatcgcg ctgccgtgct aatggaaagc cagatgcagc
     9061 aactgattgg tattctggtg cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag
     9121 tgcgcgaagc ggtcgatttt ctccactact atgccggaca ggtgcgggat gatttcgcta
     9181 acgaaaccca ccgtccatta gggcctgtgg tatgtatcag tccgtggaac ttcccgctgg
     9241 cgattttcac cggacagatc gctgccgcac tggcggcagg taacagcgtg ctggcaaaac
     9301 cggcagaaca aacgccgctg attgccgcgc aagggatcgc cattttgctg gaagcgggtg
     9361 taccgccagg cgtggtgcaa ttgctgcccg gtcggggtga aaccgtgggc gcgcaactga
     9421 cgggtgatga tcgcgtgcgc gggatgatgt ttaccggttc aaccgaagtc gctacgttac
     9481 tgcagcgcaa tatcgccagc cgcctggacg ctcagggtcg ccctattccg ctcatcgctg
     9541 aaaccggcgg catgaacgcg atgattgtcg attcttcagc actgaccgaa caagtcgtaa
     9601 tagatgtgct ggcctcggcg ttcgacagtg caggtcagcg ttgttcggca ctgcgcgtgc
     9661 tgtgcctgca agatgagatt gccgaccaca cgctgaaaat gctgcgcggc gcaatggccg
     9721 aatgccggat gggtaatccg ggtcgcctga ccaccgatat cggtccggtg attgatagcg
     9781 aagcgaaagc caatatcgag cgtcatattc agaccatgcg tagtaaaggc cgtcaggtgt
     9841 tccaggcggt gcgggaaaac agcgaagatg cccgtgaatg gcaaagcggc acctttgtcg
     9901 ctccgacgct gatcgaactg gatgactttg ccgaattaca aaaagaggtc tttggtccgg
     9961 tgttgcatgt ggtgcgttac aaccgtaacc agctgccggc gctgatcgag cagattaacg
    10021 cttccggtta tggtctgacg cttggcgtcc atacgcgcat tgatgaaacc atcgcccagg
    10081 tcactggctc ggcgcatgtc ggtaatctgt acgttaaccg taatatggtg ggcgcagtgg
    10141 ttggggtgca accgttcggc ggcgaagggt tgtccggtac cgggccgaaa gcaggcggtc
    10201 cgctctatct ctaccgtctg ctggcgaatc gcccggaaag tgcgctggcg gtgacgctcg
    10261 cgcgtcagga tgcggagtat ccggtcgatg cgcagttgaa agccgcattg actcagccgc
    10321 taaatgcact gcgggaatgg gcggcaaacc gtccagaatt gcaggcgtta tgtacgcaat
    10381 atggcgagtt ggcgcaggca ggaacacaac gattgttgcc agggccgacg ggtgaacgca
    10441 acacctggac gctgctgccg cgtgagcgcg tgttgtgtat tgcggatgat gaacaggatg
    10501 cgctgactca gctcgccgcc gtgctggcgg tgggtagcca ggtgctgtgg ccggatgatg
    10561 cgctgcatcg tcagttagtg aaggcattgc catcggcagt cagcgaacgt attcaactgg
    10621 cgaaagcgga aaatataacc gctcaaccat ttgatgcggt gatcttccac ggtgattcgg
    10681 atcagcttcg cgcattgtgt gaagccgttg ccgcacggga tggtgcaatt gtttcggtgc
    10741 agggttttgc ccgtggcgaa agcaatatcc ttctggaacg gctgtatatc gagcgttcgc
    10801 tgagtgtgaa taccgctgct gctggcggta acgccagttt aatgacgatc ggttaaacag
    10861 gtagccggag gatgttacaa cctcctccgg catctttaac gtggtcgaat cccctcaata
    10921 ataatccgct gcacgttttc aaccgtttga ttgaaaaaca cctcatcgcg caacgtcgcg
    10981 cctgtcaccg cctccacctg aggggcaaaa tcggcgtaat gttgagtgga agcccaaatc
    11041 ataaaaatca aatgctgcgg gtcaatcggc gcgagtttgc cgcttttaac ccaaccggca
    11101 atgagcgccg atttctcatc aattaatgcc ttcaaatcgc ccgtcagttc atccattaac
    11161 agcggtgcac ctgccagcat ctccatacag aacaggcgcg aagcctgcgg ataatcgcgg
    11221 gaaacttcca gcttcagacg gatgtattct ttgatcgccg ccagcggggc gaaatcttct
    11281 cgaaacgctt ttaacggtgc cagccagata tcgagaatct gccgcagtac ggctatatac
    11341 agcgcctctt ttgacggaaa gtaatacagc agattggttt ttgaaacacc cgccaactcc
    11401 gcgatctgct ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg
    11461 ctaagaatcg cttttttctt cgcgcttact gcgcgcgaac gcttacccgt tgttttcact
    11521 gcgccttgcg tcatgcgctc tcccctcttg ttgattggca acaggatagc aaaacccccg
    11581 cccggcctcg accttctgca ctctcatcgc gcacgaatgc atgttttatg tgcaactgtt
    11641 ttgaccgttt agtccacttt tttttaggca ttttagacaa cttaacatta acacattaat
    11701 aacattcact ttttaaaaac tggcatccgc tttgcaaaca agccattacg cggctgaaaa
    11761 ggatatgcag gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat
    11821 gatgaaaatt ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc
    11881 gccgcagtac atgccgacat ttgaactgaa taaagccatt gtgcaaaaag cggagcacta
    11941 ccatttcgat ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt
    12001 ctgggatcac aaccttgagt cgttcacctt gatggcgggg ctggcagccg tcacctcacg
    12061 cattcagata tacgcgacag ctgccacctt aacgttacct ccggcaatcg tcgcccgtat
    12121 ggccgcaacc atcgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg
    12181 gcagaagccc gagtatgagc agatggggat ctggcctggc gatgactatt tctcccgtcg
    12241 ttacgactat ctcaccgaat atgttcaggt gctgcgcgac ctgtggggct cggggaaaag
    12301 cgattttaaa ggcgattttt tcactatgga tgattgtcgc gtcagtccgc aaccgagtgt
    12361 ccccatgaaa gtgatatgcg ccgggcaaag cgacgctggc atggcgttct ccgcccggta
    12421 tgccgatttt aacttctgtt tcggcaaagg cgtaaataca cccacggctt tcgccccgac
    12481 cgctgcgcgg atgaaacagg ccgcagagca aaccggacgc gacgttggct cttatgtgtt
    12541 gtttatggtg attaccgacg aaaccgacga tgccgctcgt gcaaaatggg aacactacaa
    12601 agcgggcgcg gatgaagagg cgttaagctg gctaactgaa caaagtcaga aagatacccg
    12661 ctcaggtact gacaccaacg tccgtcagat ggccgatccc acttcggcgg taaacatcaa
    12721 tatggggacg ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg aagtcgcaaa
    12781 cgtgcctggt gccgaaggcg tgctgttgac cttcgatgat tttctgtcgg gaatcgaaac
    12841 cttcggcgag cgcattcaac cactgatgct gtgccgcgcc catctccctg cgctgactca
    12901 ggaggtggca tgatgacaac cttaaccgca cgaccggaag ccattacctt cgatccgcag
    12961 caaactgcgc tgatcgtggt ggatatgcaa aatgcctatg ccacgccagg cggctactta
    13021 gatctcgccg ggtttgatgt ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc
    13081 gtgaccgcag cgcgaacggc agggatgctg atcatctggt ttcaaaatgg ctgggatgaa
    13141 cagtatgtcg aggctggcgg ccccggctca ccgaattttc ataaatcgaa cgccctgaaa
    13201 accatgcgta atcagccgca gctgcagggg aaattgctgg cgaaaggctc ctgggattat
    13261 caactggtgg atgaactggt gccgcagcct ggcgatattg tgctgccgaa gccgcgctac
    13321 agcggtttct tcaatacgcc gctggacagc attttgcgca gccgtggaat acgccatctg
    13381 gttttcaccg gtatcgctac caacgtctgc gtcgaatcga cgctacgcga cggctttttt
    13441 ctggagtatt tcggcgtggt gctggaagac gcaacacacc aggcggggcc ggaatttgca
    13501 cagaaagccg cgttgttcaa tatcgaaacc ttttttggct gggtcagcga cgtcgaaacg
    13561 ttctgcgacg cgctttcttc cacgtccttt gctcgtatcg cttaaggagt ttaacgatgc
    13621 caaaatccgt aattattcct gctggcagca gcgcaccgct ggcccccttc gttcccggca
    13681 cgctggctga tggcgtggtg tatgtctccg gtacgctggc ttttgatcaa cataacaacg
    13741 tgctgtttgc cgatgaccca aaggcgcaaa cccgccacgt tctggaaacc atccgcaagg
    13801 tcatcgagac ggcgggtggc acgatggcgg atgtgacctt caacagcatc tttattaccg
    13861 actggaaaaa ttacgccgcg attaacgaaa tctacgccga gtttttcccg ggtgacaaac
    13921 cggcgcgatt ctgcattcag tgcggactgg taaaacctga cgcgctggtg gaaatcgcca
    13981 caattgcgca tatcgccaag tgaggccgcg atgaaacttt cactctcacc tcccccttat
    14041 gctgatgcgc ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag ttactggtta
    14101 ccgcaactgg cggtgctgga tcaggagtat caggtagtct gttacgacca gcgcggcacc
    14161 ggcaataatc ccgacacgct ggcagaagat tacagtatcg cccagatggc agcggaactg
    14221 catcaggcgc tggtagccgc agggattgag cgttacgcgg tggtcggcca tgcgctcggt
    14281 gcgctggtgg gaatgcagct ggcgctggat tatcccgcgt cggtaactgt gctggtcagc
    14341 gttaacggct ggctacgaat aaacgcccat acgcgccgct gttttcaggt tcgcgaacag
    14401 ttactacata gcggcggcgc gcaggcatgg gtggaagcgc agccgttgtt cctctatccc
    14461 gccgactgga tggcggcccg cgcacctcgc cttgaggcag aagacgcgct ggcactggcg
    14521 cattttcagg gcaaaaataa tttactgcgt cgacttaacg ccctcaaacg cgctgacttt
    14581 agtcgccatg cggatcgtat ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg
    14641 ctggtgccat cagcatgttc cagtgaactt catgccgctc tgcccgatag ccagaaaatg
    14701 gtgatgcgct atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg
    14761 ttactcaacg ggcttgccag cctgttacat caccgtgaag ccgccctgta aggaattgct
    14821 atgaacgaag ccgttagccc aggtgcgctt agcaccctgt tcaccgatgc ccgcactcac
    14881 aacggctggc gggagacacc cgtcagcgat gagacgttac gggagattta tgccctgatg
    14941 aaatgggggc caacatctgc taactgttct ccggcacgga tcgtgtttat ccgcactgca
    15001 gaaggaaaag aacgtcttcg cccggcgctt tccagcggca atctgcaaaa aaccctgacc
    15061 gcgcccgtca ccgctatcgt cgcctgggac agtgaatttt atgaacggtt accacagctg
    15121 tttcctcacg gcgatgcccg cagttggttt acctccagtc cacaacttgc cgaagaaaca
    15181 gcgtttcgca acagttccat gcaggcggcc tatctgatcg tcgcctgccg tgcgctgggg
    15241 ctggataccg gcccgatgtc gggctttgac cgtcaatacg ttgacgacgc cttttttgcg
    15301 ggcagcacgc tgaagagcaa tctgctgatt aatatcggct atggcgattc cagcaaactt
    15361 tttgcgcgcc tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac
    15421 aatgaacatt gtcgatcaac aaacctttcg cgatgcgatg tcctgtatgg gcgcggcggt
    15481 caatatcatc actacggacg gtccagccgg gcgcgccggg ttcaccgcca gcgccgtctg
    15541 cagcgtgacc gacacgccgc ccacattact ggtgtgcctg aatcgtgggg cgtccgtctg
    15601 gccggtattc aatgaaaatc gaacgctgtg tgtaaataca cttagcgccg ggcaggagcc
    15661 gctttcaaac ctttttggcg gcaaaacgcc catggaacac cgcttcgccg ccgcccgctg
    15721 gcagactggt gtgaccggat gcccgcaact ggaagaggca ctggtttcgt ttgactgccg
    15781 tatcagccag gtggtgagcg tcggcaccca cgacattttg ttttgcgcca tcgaagcgat
    15841 tcatcgtcac gccacaccct acgggctggt gtggttcgat cgcagttatc acgcgctgat
    15901 gcgccctgtt tgttaacctc cttaaggaga cagctgatgg caatgttcgg ttttcctcac
    15961 tggcagttaa aatcgacctc tacagagagc ggcgtggtcg cgccggatga acgattgccg
    16021 tttgctcaga cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg
    16081 ttaatgccga ttctgatggg gctggatccc aatctttcca ttttaatgtc ggggatcggc
    16141 acgttactgt ttttctttat taccggcggg cgcgtgccca gttatctcgg ttccagcgcg
    16201 gcttttgtcg gcgtggtgat tgccgcaacg ggctttaatg gtcagggaat taatccgaat
    16261 atcagcatcg cgctgggcgg gattatcgcc tgtgggctgg tttataccgt cattggcctg
    16321 gtggtgatga aaatcggtac gcgctggatt gaacgactaa tgccgccggt tgtgacgggt
    16381 gcggtagtga tggcgattgg cctgaactta gcgcctatcg cagtgaaaaa tgtatcggct
    16441 tcggcctttg atagctggat ggcggtaatg acggtgctgt gtattgggct ggtggccgta
    16501 tttacccgtg gcatgatcca gcgattattg attctcgtag gattaattgt tgcctgtctg
    16561 ctgtatggcg tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc
    16621 agccatgctg cctggttcgg tctgccacat ttttccactc ctgcttttaa tggtcaggca
    16681 atgatgctga ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag
    16741 gcggtcgccg ggatgaccgg acgcaatatg gacccgtata tggggcgggc attcgtaggc
    16801 gatgggctgg cgacgatgct ttctggctct gtcggcggta gcggcgtaac cacctacgcc
    16861 gagaatattg gtgtgatggc cgtgacaaaa gtctactcga cgctggtgtt tgttgccgct
    16921 gccgtcatcg ccatgctgtt gggcttttcg ccgaaatttg gcgcgctgat ccatactatt
    16981 ccggccgcgg tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc
    17041 gcacggatct gggtacaaaa cagtgtggat ttaagccaga acggtaattt gattatggtc
    17101 gcagtaacct tagtgctggg tgcgggtgat tttgctctca cactgggcgg ttttacgttg
    17161 ggagggatcg gtacagccac ctttggcgcg attttactca atgcgttgtt aagcagaagg
    17221 ttggttgacg ttccaccgcc agaagttgtt catcaggagc cataaacaga aaaccgccgg
    17281 ggtaaccggc ggtgatggcg ttttgacgag ctacagacaa aaatgcatgc gtcattgatt
    17341 agcccggcta gttgtcgctt ttaccgtgac tgctcttgcc acctttttta cctgcttcag
    17401 atgcgcgctg cgggtcattt ttgaaattac ccccgctgtg ctgtccacct tttttacctg
    17461 cttctgatgc tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca
    17521 ttactgacct ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg
    17581 gcagccatat tttcgctacg tgggactaag cttagtaaac tgacgcgggc agacccgcgt
    17641 ttggtaatat cctgcaacag cttcttagcg agaatagata tcatctccaa taattatcca
    17701 taagccgctc ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa
    17761 attacgtgct tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga
    17821 aaaggacgtc ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga
    17881 ggagtggtta gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga
    17941 aaccatgatg cattaaaaac acagaattac cgaagatcgt ccaagagcac atatttttca
    18001 atctgttaca cctcattttg accatgaaga aatcgatttt acaccgttat ttatacgttc
    18061 ttttttgccc caaatatgcc ccaaacaacc acattttgcc ccaatagtgc cccaaaaatt
    18121 taataggatt taagtagata tttcagacaa ttaacgcaag tttacgatgt gaatttacgt
    18181 taattgtccg ggttgggtat ataccgtcac tcgttcatca atatttccgc agattgacaa
    18241 atccgcctga gctatcgcat actgaccgca ctgaacaaat aagcggtcat ccgcacccga
    18301 tagctttgcg gcttttttat ttatatcggg cttctcatga aaaacctttt aaataatttt
    18361 cctgacaatt gcattgaaaa tattttacca aaagatgatt ttggttatgg gcgtctttta
    18421 atcggctcaa agggtaaacg tattaaaact ggcgcgcata ggttaattta ccgaattttt
    18481 attggagaaa ttcctgcggg catggttgtc aggcattctt gcgataatcc tgcatgttgt
    18541 aatccaaagc atttactaat aggaacaatc cttgataata atgctgatcg acagaaacga
    18601 aaacgccaag cacgtcatga aaaacatggg cgagcaaaac taactaaaga gcaagtgcta
    18661 tttataagag ctaatgaaat gagtatacca aattcagttc ttgcagataa atacagggtc
    18721 tccaatagct taatatggca aataaaaaac tatcgcattt ggaatgaatt gtaaccacgc
    18781 tgttgacgac agcatccaat agtgactata ctccgcgccg agacctcgaa aacctcccaa
    18841 aagcggatta agccaacccc gtcagtgttg gattttttat gcctgtcatt cagtgatggc
    18901 acaatgtgcg gtcacatccc cgatccatgt cgggagggcg acgaatacaa cacccgcaag
    18961 gggaataagt ccgcggtatc ttttgggccg tttcgagcct cccggcacca cattttgtgg
    19021 tggcatttct cgaaaaaagc caaaggaggt catcatgacc actcaactca tccctgtgtt
    19081 tgaaggtaca atctcaaatg aacctacgct tctggtgaac gcgcgtgatt tgcatgggtt
    19141 tttagaggta gataaagatt tttctaactg gatacgggcc aggctaaacg agtatggatt
    19201 cgtagaaaat ttagactaca ttctattttc gccaaatttg gcgaaaactc taggccgccg
    19261 ccgcaaggac taccatctca ccctggacac agccaaagag cttgcgatgg ttgaacgaaa
    19321 cgaaaaaggc cgccagatcc gccgctactt catcgagtgc gaaaagaaac ttcgcaacat
    19381 tcagccagta caagcagaat cacagccaca atttaccgcc gaggaaatca tcctcctttg
    19441 ctacatgcaa ctctggatgg aaaaagccca ggaccttagc aagcagctgt accccataat
    19501 gaaagagctg aactcctcat acacgaacaa gctgtatgac attgcgtttg agactatcta
    19561 catggtgacg aagaacagag acgcgctact aagggaggta acacgtctcg acatgtcaag
    19621 ttccgttatc cagcgggcca tgccaatgct gaaaagcctg cgggcaagac aatttgaatt
    19681 ctgaaaccaa aggagcttcg gctccttttt tcatgcctga acgacaacgc cgctgaaatc
    19741 gaagagttaa tacaataaag agaagagctc agtacattag ataaacattt tttatcatta
    19801 tctcattgcc tttattcaaa aaaatagtta cataacgtta ctaggtaaaa tgcaaaccct
    19861 cgctattttt atcagattgt atgccggtaa ctataacaaa aatcagaact ggagaagatg
    19921 ttttgtcagc agccattcac cgcattgaat ggttgtttga aacattctct tctgtatgtt
    19981 tatctttttc tggcggtaaa gattccactg tacttttcca tcttgtagcc gaagtggcct
    20041 acaggaagaa acgtcgtttt tctgtattgt tcattgactg ggaagcccag tatcaatgca
    20101 ccattgatca tatacagaag atgcggatga tgtaccaaga tgtaacagat accttttact
    20161 gggttgcact tcccctgacg acagtaaatg gtgtctcgca gtttcagcct gagtggatat
    20221 gctgggagcc cggtgtagag tgggttcgac agccaccgga agatgctatt actgatatgg
    20281 cgtatttccc cttttaccgc tacgccatga cttttgaaga atttgtccca gctttttctt
    20341 cctggttcgc aagtaaccaa tgtagcgtgg caatactgac tggagtccgc gcggatgaat
    20401 ctcttaacag atttatggga ctggtttccc aacgaaaact gagatatgca gatgataaac
    20461 cctggaccac ggcgtcacct gaaggttttt actacaccat gtatccgctg tatgactgga
    20521 aaaccaacga tatctgggta tataacgcta aaacctgtgc aatctacaat cctctgtatg
    20581 atttgatgta ccgtgcaggc attcctctac gtaatatgcg tgtctgtgag ccttttggcc
    20641 cggaacagcg taagggacta tggctttatc atgttctgga gccagaaact tgggcacgga
    20701 tgtgtgaacg agtatctggt gctgccagtg gggctcttta tgccaatgaa agcggggcct
    20761 attttgccct gcgtaaacgg atgtcaaaac catcccatca tacctggcgc agctatgcgt
    20821 tcttcctgct ggacgtaatg ccggaaagaa cggctgaaca ctaccgcaac aagatagcag
    20881 tctacctgcg ctggtatcag aaccggggat tcccggatga tatcccggat gaacaggaaa
    20941 atgatctggg ctgccgggat gttccctcct ggcgacgtat ttgtaagact ctcataaaga
    21001 atgatttctg gtgccgaacg ctctccttca gccccaacaa accccggcac tatgaacgtt
    21061 acctgcagcg tatgaatgaa aggaggaagg aatggggaat tctgtgacac cagaggtgga
    21121 ggtgctgagc aagatgattc gacagtactt cagtcaggaa caatctgaag agaaaaccat
    21181 acaggccctg aaccatctcc gctgcgtact ccatgagatc agcccctttg ctcaagaacc
    21241 agtggactgt gtgctgtggg taaaagcgga tgaaattgtc gctaacgatt ataatcccaa
    21301 tgtcatggcc ccaagcgaaa aaaaattact gaaacaatct ctggaaaaag acggttttac
    21361 tcaaccaatt gttgtttcgg aagaaacaag ccattacctg gtggtggatg gttttcatag
    21421 gcagttacta ggtagaagaa cagttactgg aaaacgcctg aaaggctggc taccagtaac
    21481 ctgtattaat ccagaccgaa agggacaaac ctcacgtatt gccgcgacta tccgacataa
    21541 cagagccagg gggaaacacc agataacctc aatgtctgat attgtcagag atctttcacg
    21601 tcttggctgg actgatgaac gtatcggaac agaactgggg atggaccagg atgaagttct
    21661 gaggttaaag cagattagcg ggctaacgga gttatttcag caggcagatt tcagccctgc
    21721 atggactgtt acataaagtt aggttaggcc tgtggagccg gggaatcgga attaaacatc
    21781 ttccctcccc ggtaaataca aataagggct gaacatcatc ccgactacct cagatcacct
    21841 aataatatat caagattatt ttgcgcatta atgctattcg ccattactgt tgctgcttct
    21901 ggcaactttc caagagcaat aaatatatcc ttaaggctga ttggaattga tgaaccatgc
    21961 ggatcgcgcc actcaccgca aattctgtga gtttcatcac aaagcctgaa cgcatcccaa
    22021 cctccatatt gaccaaaaat tatgtccatt gactctattt ctg
//
LOCUS       DAZGJT010000079        21715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZGJT010000079 DAZGJT010000000
VERSION     DAZGJT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.079"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            208..543
                     /gene="yjdM"
                     /locus_tag="ACHKFK_003310"
     CDS             208..543
                     /gene="yjdM"
                     /locus_tag="ACHKFK_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO9053579.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            703..1146
                     /gene="yjdN"
                     /locus_tag="ACHKFK_003311"
     CDS             703..1146
                     /gene="yjdN"
                     /locus_tag="ACHKFK_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO9053580.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTH"
     gene            1279..2067
                     /gene="phnC"
                     /locus_tag="ACHKFK_003312"
     CDS             1279..2067
                     /gene="phnC"
                     /locus_tag="ACHKFK_003312"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO9053581.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSVGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSYFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGCSQQFDNERFDHLYRSINRVEENAKAA"
     gene            2092..3108
                     /gene="phnD"
                     /locus_tag="ACHKFK_003313"
     CDS             2092..3108
                     /gene="phnD"
                     /locus_tag="ACHKFK_003313"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9053582.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLNPAYAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQSVKDNKGLNEQDKLAKTTAIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3214..3993
                     /gene="phnE"
                     /locus_tag="ACHKFK_003314"
     CDS             3214..3993
                     /gene="phnE"
                     /locus_tag="ACHKFK_003314"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO9053583.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVIAVLIVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4014..4739
                     /gene="phnF"
                     /locus_tag="ACHKFK_003315"
     CDS             4014..4739
                     /gene="phnF"
                     /locus_tag="ACHKFK_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO9053584.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     CFDSGSLHDFLREQTGITLRRSQTRISARRAQAKECQHLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4740..5192
                     /gene="phnG"
                     /locus_tag="ACHKFK_003316"
     CDS             4740..5192
                     /gene="phnG"
                     /locus_tag="ACHKFK_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO9053585.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNINADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVQGRDKQHAERCALIDALM
                     QQNRHFQNLSETLITPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5189..5773
                     /gene="phnH"
                     /locus_tag="ACHKFK_003317"
     CDS             5189..5773
                     /gene="phnH"
                     /locus_tag="ACHKFK_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO9053586.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVNQSLRFHTNAPLVNQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVTSLSGGRMLRLTGAGIAEERMIAPQLPKCILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5773..6837
                     /gene="phnI"
                     /locus_tag="ACHKFK_003318"
     CDS             5773..6837
                     /gene="phnI"
                     /locus_tag="ACHKFK_003318"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO9053587.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTGMRLE
                     RRISAIYKDIPGGQLLGPTYDYTHRLLDFTLLANGETPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDIFIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6830..7675
                     /gene="phnJ"
                     /locus_tag="ACHKFK_003319"
     CDS             6830..7675
                     /gene="phnJ"
                     /locus_tag="ACHKFK_003319"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO9053588.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQN
                     EAKSQ"
     gene            7672..8430
                     /gene="phnK"
                     /locus_tag="ACHKFK_003320"
     CDS             7672..8430
                     /gene="phnK"
                     /locus_tag="ACHKFK_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO9053589.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIRYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVMELNLAVVIVTHDLGVARLLADRLLV
                     MKQGKVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8541..9221
                     /gene="phnL"
                     /locus_tag="ACHKFK_003321"
     CDS             8541..9221
                     /gene="phnL"
                     /locus_tag="ACHKFK_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO9053590.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQGASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKARGTAIVGIFHDETVREQVADR
                     LHPMGVSS"
     gene            9218..10354
                     /gene="phnM"
                     /locus_tag="ACHKFK_003322"
     CDS             9218..10354
                     /gene="phnM"
                     /locus_tag="ACHKFK_003322"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO9053591.1"
                     /translation="MIINNVKLVLENEVVHGSLEMQDGEIRAFAESQSHLSEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAM
                     CRARHIALASHDDATHAHVAQSHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDQSNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10354..10911
                     /gene="phnN"
                     /locus_tag="ACHKFK_003323"
     CDS             10354..10911
                     /gene="phnN"
                     /locus_tag="ACHKFK_003323"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO9053592.1"
                     /translation="MTGKLIWLMGPSGSGKDSLLAELRQREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10898..11332
                     /gene="phnO"
                     /locus_tag="ACHKFK_003324"
     CDS             10898..11332
                     /gene="phnO"
                     /locus_tag="ACHKFK_003324"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO9053593.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHQAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11334..12092
                     /gene="phnP"
                     /locus_tag="ACHKFK_003325"
     CDS             11334..12092
                     /gene="phnP"
                     /locus_tag="ACHKFK_003325"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO9053594.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGNQIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIEVE"
     gene            complement(12120..12449)
                     /gene="yjdP"
                     /locus_tag="ACHKFK_003326"
     CDS             complement(12120..12449)
                     /gene="yjdP"
                     /locus_tag="ACHKFK_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO9053595.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12518..12967)
                     /gene="rpiB"
                     /locus_tag="ACHKFK_003327"
                     /gene_synonym="alsI"
     CDS             complement(12518..12967)
                     /gene="rpiB"
                     /locus_tag="ACHKFK_003327"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO9053596.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERDVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEADGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQQRN"
     gene            13326..14216
                     /gene="alsR"
                     /locus_tag="ACHKFK_003328"
     CDS             13326..14216
                     /gene="alsR"
                     /locus_tag="ACHKFK_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO9053597.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALAVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14275..15210
                     /gene="alsB"
                     /locus_tag="ACHKFK_003329"
     CDS             14275..15210
                     /gene="alsB"
                     /locus_tag="ACHKFK_003329"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO9053598.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEGFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKVLVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVDAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15337..16869
                     /gene="alsA"
                     /locus_tag="ACHKFK_003330"
     CDS             15337..16869
                     /gene="alsA"
                     /locus_tag="ACHKFK_003330"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
                     /protein_id="HFO9053599.1"
                     /translation="MATPYISMAGIGKSFGPVHALKSVNLTVYPGEIHALLGENGAGK
                     STLMKVLSGIHEPTKGTITINNINYNKLDHKLAAQLGIGIIYQELSVIDELTVLENLY
                     IGRHLTKKICGVNIIDWREMRVRAAMMLLRVGLKVDLDEKVANLSISHKQMLEIAKTL
                     MLDAKVIIMDEPTSSLTNKEVDYLFLIMNQLRKEGTAIVYISHKLAEIRRICDRYTVM
                     KDGSSVCSGMVSDVSNDDIVRLMVGRELQNRFNAMKENVSNLAHDTVFEVRNVTSRDR
                     KKVRDISFSVCRGEILGFAGLVGSGRTELMNCLFGVDKRAGGEIRLNGKDISPRSPLD
                     AVKKGMAYITESRRDNGFFPNFSIAQNIAISRSLKDGGYKGAMGLFHEVDEQRTAENQ
                     RELLALKCHSVNQNITELSGGNQQKVLISKWLCCRPEVIIFDEPTRGIDVGAKAEIYK
                     VMRQLADDGKVILMVSSELPEIITVCDRIAVFCEGRLTQILTNRDDMSEEEIMAWALP
                     QE"
     gene            16848..17828
                     /gene="alsC"
                     /locus_tag="ACHKFK_003331"
     CDS             16848..17828
                     /gene="alsC"
                     /locus_tag="ACHKFK_003331"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO9053600.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSS
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGI
                     DPFLAALIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            17839..18534
                     /gene="alsE"
                     /locus_tag="ACHKFK_003332"
     CDS             17839..18534
                     /gene="alsE"
                     /locus_tag="ACHKFK_003332"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO9053601.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLASKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RRHGMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKVAELKAW
                     REREGLEYEIEVDGSCNQATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTAQIL
                     AAKSEVQPHAKTA"
     gene            18518..19447
                     /gene="alsK"
                     /locus_tag="ACHKFK_003333"
     CDS             18518..19447
                     /gene="alsK"
                     /locus_tag="ACHKFK_003333"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO9053602.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKQTAEVIAPD
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDGVILGGGVMDMPAFPRETLIAMTQKYLRRPLPYQVV
                     RFIAASSSDFNGAQGAAILAHQRFLPQSCAKVP"
     gene            19719..21704
                     /gene="yjcS"
                     /locus_tag="ACHKFK_003334"
     CDS             19719..21704
                     /gene="yjcS"
                     /locus_tag="ACHKFK_003334"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO9053603.1"
                     /translation="MNNSRLFRLSRIVIAFTAASGMMINTAYATDEAKAATQYTQQVN
                     QNYAKSLPFGDRQDFDDAQRGFIAPLLDEGILRDANGKIYYRANDYKFDINAAAPETV
                     NPSLWRQSQINGISGLFKVTDKMYQVRGQDISNITFVEGEKGIIVIDPLVTPPAAKAA
                     LDLYFQHRPQKPIVAVIYTHSHADHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLPHNAQGNVGNGLGVTLATGDPSIIAPTKTIVRTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMAVRLDSTKA
                     AGKNISLNFNMSNGDNLNLMLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         5207 a         5969 c         5781 g         4758 t
ORIGIN      
        1 acaagtagaa cattaacctt atcttaaata ataaatggag gcaaaaagat tattttcttt
       61 ttgcgtttcc tttcaaatga aaacgatcgt cgtctaaaat cagcagtacc cccgacaaac
      121 tcagggattt tgtgtataat tgcggccttt ttcggcaatc tgccgttttt tggcgctttt
      181 gccctgctga cttttgagga aatccacatg tcattaccac actgcccaaa atgcaactcc
      241 gaatacacct acgaagataa cggcatgtac atctgcccgg aatgtgccta cgaatggaac
      301 gacgcagaac ctgcacagga aagcgacgag ctgatcgtta aagatgctaa cggcaatctg
      361 ctggctgacg gcgacagcgt gaccatcatt aaagatctga aggtgaaagg tagctcttcg
      421 atgctgaaaa ttggcaccaa agtgaaaaac atccgcctgg ttgaaggcga ccataacatc
      481 gattgcaaaa tcgacggttt tggtccgatg aaactgaaat ctgagtttgt gaaaaagaac
      541 tgattgtatt gtgatcggta agccggataa ggcgctcgcg ccgcatccgg caacgatgcc
      601 gactgcctga tgcgacgctg accgcgtctt atcaggcttc cctctcatct gtataaattt
      661 cgaactacac ttaactggct tctcttaact gaggtcacca tcatgccgtt aagtccctac
      721 ctctcttttg ccggtaactg ttccgacgcg attgcctatt atcaacgtac gttgggcgcg
      781 gaactgctct ataaaatcag cttcggcgaa atgccgaaat cagcgcagga cagcgccgag
      841 aactgccctt ccggaatgca atttcccgat accgccatcg ctcatgccaa cgtgcgcatt
      901 gcaggaagcg acatcatgat gagcgatgct ataccgtcag gaaaagccag ctactccggc
      961 tttacgctgg tgctcgattc gcaacaggtc gaagaaggaa aacgctggtt tgacaacctt
     1021 gccgctaacg gaaaaatcga aatggcctgg caggaaactt tctgggcgca tggcttcggc
     1081 aaagtcaccg ataaatttgg cgtaccgtgg atgattaatg tcgtcaaaca acaaccaacg
     1141 cactaacccg ccgggagggc cgccctcccg cactgtcatc gaaatctcgt taactcttca
     1201 tccgttagtc acttttagtt aacaaaatcg tcacaataat ccgccacgat ggagccactt
     1261 ttttagggag gctgcatcat gcaaacgatt atccgtgtcg agaagctcgc caaaaccttc
     1321 aatcagcatc aggcgctgca cgcggttgat ctgaacattc atcatggtga aatggtggct
     1381 ctgcttgggc cgtcgggttc cggaaaatcc actcttttac gtcacttaag cggtttgatt
     1441 accggcgata aatctgtcgg tagccatatc gagctgctgg gccgcactgt ccagcgcgaa
     1501 ggccgtctgg cccgcgatat ccgcaaaagc cgcgcccata ccggctacat ctttcaacag
     1561 ttcaacctgg tgaatcgcct gagcgtactg gagaacgtgc tgattggcgc gctcggcagc
     1621 acgccattct ggcgaacctg ttttagctac ttcacccgcg agcagaaaca gcgcgcgtta
     1681 caggcgctga cccgcgttgg catggtgcac tttgcccatc aacgtgtttc cacactctcc
     1741 ggcggccagc aacagcgtgt ggcgattgcc cgcgcgctga tgcagcaggc gaaggtcatt
     1801 ctcgctgacg aacccatcgc ctcgctggac ccggaatcgg cacgcatcgt gatggacacc
     1861 ctgcgcgaca tcaaccagaa cgacggcatt accgtggtcg tcacgctgca tcaggtggat
     1921 tacgccctgc gctactgcga acgcatcgtc gccctgcgcc aggggcacgt cttctacgac
     1981 ggctgcagcc aacagtttga taacgaacgt tttgaccatc tctaccgcag cattaaccgc
     2041 gtcgaagaga acgcgaaagc tgcctgacat ccccatcatt gaggaaaacg aatgaacgct
     2101 aagataattg cctcgctggc cttcaccagc atgttcagcc tcagcaccct gttaaatccg
     2161 gcatacgccg aagagcagga aaaggcgctg aatttcggca ttatttcaac ggaatcacag
     2221 caaaacctga aaccgcaatg gacgccgttc ttacaggata tggagaagaa gctgggcgta
     2281 aaggtcaacg ccttctttgc cccggactac gcgggcatta tccaggggat gcgcttcaat
     2341 aaagtggata tcgcctggta cggcaacctg tcggcgatgg aagcggtgga tcgcgccaac
     2401 ggtcaggtct tcgcccagac ggtcgcggcg gatggatcgc cgggttactg gagcgtgttg
     2461 atcgtcaaca aagatagccc gatcaacaac ctgaacgatc tgctggcgaa gcggaaagat
     2521 ctcaccttcg gcaatggcga tcctaactct acctccggct tcctcgtccc cggttactac
     2581 gtcttcgcta aaaacaatat ctccgccagc gactttaagc gcaccgtcaa cgccgggcat
     2641 gaaaccaacg ccctcgccgt cgctaacaag caagtggatg tcgccaccaa caacaccgaa
     2701 aacctcgaca agctgaaaac ctccgcgccg gagaagctga aagaactgaa ggtgatctgg
     2761 aaatcaccgc tgatccctgg cgatccgatc gtctggcgca aaaatctttc cgaaaccact
     2821 aaagacaaga tctacgactt ctttatgaat tacggcaaaa cgccggaaga gaaagcggtg
     2881 ctggagcgcc tgggctgggc gccgttccgc gcctccagcg acttgcaact ggtgccgatt
     2941 cgccagctcg cactgtttaa agagatgcaa agcgtgaagg acaacaaagg gctgaacgaa
     3001 caggacaagc tggcaaaaac caccgccatt caggcgcaac tggatgattt ggaccgcctg
     3061 aacaacgcgt taagcgcaat gagttcggtg agtaaagcgg tgcagtaaat cgtaggtcgg
     3121 ataagacgcc ccggcgtcgc atccgacaat gtgcagacgt tgatgccgga tgcggtgcaa
     3181 gcaccttatc cggcctacag accggagcca aacatgcaaa ccatcaccat cgccccaccc
     3241 aagcgcagct ggttctcgct tctgagctgg gccgttgtta tcgccgtgct gatcgtctcg
     3301 tggcagggcg cggaaatggc cccgcttacg ctgattaaag atggcggcaa catggcgacg
     3361 ttcgccgccg acttcttccc gcccgatttc agccagtggc aggattacct caccgaaatg
     3421 gccgtcacgc tgcaaatcgc cgtctggggc accgcgctgg cggtggttct ctccatcccc
     3481 ttcggcctga tgagcgctga aaacctggta ccgtggtggg tctaccaacc tgtacgccgc
     3541 ctgatggacg cctgccgcgc cattaacgaa atggtcttcg ccatgctgtt cgtggtcgcc
     3601 gtcggcctcg gcccgttcgc tggcgtgctg gcgctgttta tccacaccac cggcgtactt
     3661 tccaaactgc tttccgaagc ggtagaagcg attgaacccg gcccggtgga gggcattcgc
     3721 gctaccggag ccaacaagct ggaagagatc ctctacggcg tactaccgca ggtcatgccg
     3781 ctgctgattt cctactccct ctatcgcttc gaatccaacg tccgctcggc gaccgtcgtc
     3841 ggtatggttg gcgcgggcgg gatcggcgtc acgctgtggg aagcgattcg cggtttccag
     3901 ttccagcaaa cctgcgccct gatggtgctc atcatcgtca cggtcagcct gctggatttc
     3961 ctctctcaac ggttgcgtaa gcactttatc tgataagcga ggcattgata tctatgcact
     4021 tgtctacaca tccgaccagc tacccaacac gctatcagga gatagccgca aaacttgagc
     4081 aggagcttcg tcaacactac cgctgcggcg actatcttcc cgccgagcag caactggcgg
     4141 cgcgctttga ggtgaaccgc cacaccctgc gccgcgccat cgaccaactg gtggaaaaag
     4201 gctgggtaca gcgccgtcag ggcgtcggcg tactggtgct gatgcgcccg ttcgattacc
     4261 cgctcaacgc ccaggcgcgt tttagccaga acctgctgga ccagggcagc catcccacca
     4321 gcgaaaaatt gctatcggtg ctgcgccccg cctccggtca cgtcgctgac gcgctgggga
     4381 taaccgaggg ggagaacgtc atccacctgc gcaccctgcg tcgggtcaac ggcgttgcgc
     4441 tctgtttaat cgaccactac ttcgcagatc tcacgctctg gccaacgctt caatgctttg
     4501 acagcggttc gctgcacgat tttctgcgcg agcaaacggg gattacgcta cgccgcagcc
     4561 agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg ccagcatctt gaaatcccga
     4621 atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg tgacggtgaa agcagcccgg
     4681 cggagtactc cgtcagcctg acgcgcgccg acatgattga attcactatg gagcactgaa
     4741 tgcacgcaga taccgcgacc cgccagcact ggatgtccgt gctggcgcac agccaaccgg
     4801 ctgaactggc agcccgcctg aacgcgctga acatcaacgc cgattatgag gtgatccgcg
     4861 ccgctgaaac tggcctggtg cagattcagg cgcgaatggg cggcaccggc gaacgctttt
     4921 ttgctggcga cgccacgctc acccgcgccg ccgtgcgtct gactgacggc acgctcggct
     4981 atagctgggt gcaggggcgc gacaaacagc acgccgaacg ttgtgcgctg attgatgcgc
     5041 tgatgcagca aaaccgccac tttcaaaact tatcagaaac ccttattacc ccgctggacg
     5101 ctgaccgtat ggcacgcatt gccgcgcgtc aggccgaagt gaacgccagc cgggtcgact
     5161 tctttacgat ggttcgcgga gacaacgcat gaccctggaa accgctttta tgcttcccgt
     5221 gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc atgagcgagc cgggcgtgat
     5281 tgtcgccctg catcagctca agcgcggctg gcagccgctg aatatcgcca ccaccagcgt
     5341 gctgctgacg ctggccgata acgacacgcc ggtgtggctt gccgcaccat tgagcaacga
     5401 tatcgtcaat cagagcctgc gttttcatac caacgcgccg ctggttaatc agccggaaca
     5461 ggcgaccttc gcggtaacag atgaggcgat ctccagcgag cagctcaacg ccctttccac
     5521 cggcactgcc gttgcaccag aagcgggcgc aacgctgatt ttacaggtca ccagcctgag
     5581 cggtgggcgt atgttgcgcc tcaccggcgc aggtattgcc gaagaacgga tgatcgcccc
     5641 gcaactgcca aagtgcattc ttcatgaact taccgagcgc ccgcacccgt tcccgctcgg
     5701 catcgacctg atcctgacct gcggcgagcg cctgctggct attccgcgaa ccactcatgt
     5761 ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaaa aggcgatcga cgccgcccac
     5821 gccctgcaag agagccgacg ccggggcgat actgatttgc ctgaactgag cgtcgcccag
     5881 attgaacagc agcttaacct cgcggtagat cgcgtgatga ccgaaggcgg tattgccgac
     5941 cgcgagctgg ccgcgctggc gctgaaacag gccagcggcg ataacgtcga agcgattttt
     6001 ctgttgcgcg cctaccgcac cacgctggcg aagctggcgg tcagtgagcc gctcgacacc
     6061 accgggatgc gtctcgaacg gcgtatttca gcaatttata aagacattcc cggcggccaa
     6121 ctgcttggcc ccacctacga ctacacccat cgcctgctcg atttcaccct gctggcaaac
     6181 ggcgaaacgc caacgctgac caccgctgac agcgaacagc aaccgtcgcc acacgtcttc
     6241 agcctgctgg cgcgtcaggg gctggcgaag tttgaagaag atagcggcgc acaaccggac
     6301 gacatcaccc gcacgccgcc ggtttatccc tgttcgcgtt cttcccgtct acaacaatta
     6361 atgcgcggtg acgaaggcta tttgttggcg ctggcctact ccacccaacg cggttacggg
     6421 cgcaatcacc cgttcgcagg cgaaattcgc agtggctata tcgatatatt tattgtgccg
     6481 gaagagctgg gatttgcggt gaacgtcggc gaattgttga tgaccgagtg tgaaatggtc
     6541 aacggtttta tcgacccgcc ggatgaacca ccgcatttta cacgcggcta tgggctggtg
     6601 ttcggcatga gcgagcgcaa agcaatggcg atggcgctgg ttgaccgtgc gttgcaggca
     6661 ccggaatacg gcgagcacgc aacaggtccg gcacaggatg aagagttcgt gctggcccat
     6721 gccgacaacg tcgaagccgc aggctttgtt tcgcatctca aactcccgca ctacgtcgat
     6781 ttccaggccg aactggagct actcaaacgt ctgcaacagg agcagaacca tggctaatct
     6841 gagcggctac aactttgctt acctcgacga gcagaccaaa cgcatgatcc gccgcgctat
     6901 cttaaaagcg gtggcaatcc ccggttatca ggtgccgttt ggcggacgcg agatgccgat
     6961 gccctacggc tggggaaccg gcggcattca gctcaccgcc agcgtgattg gcgaaagcga
     7021 cgtgttgaag gtgattgacc agggcgcaga cgacaccacc aacgccgtgt cgattcgtaa
     7081 cttctttaag cgcgtgaccg gggtgaacac caccgagcgc acggacgatg cgacacttat
     7141 ccagacgcgt caccgcatac ctgaaacgcc gctgaccgaa gatcagatag ttatcttcca
     7201 ggtgccgatc cccgaaccgc tgcgctttat tgagccgcgt gagacggaaa cgcgcaccat
     7261 gcacgcgctg gaggagtacg gcgtgatgca ggtgaaactg tatgaagata tcgcccgctt
     7321 cggtcatatc gccaccacct acgcctatcc ggtgaaggtg aacgggcgct acgtaatgga
     7381 cccgtcgccg atcccgaaat tcgataaccc gaaaatggac atgatgcccg ccctgcaact
     7441 gtttggcgcg gggcgcgaga agcgcatcta tgccgtgcca ccgtttaccc gcgtggaaag
     7501 cctcgatttc gatgatcatc cgttcaccgt ccagcagtgg gatgaaccat gcgccatctg
     7561 cggatcgacc cacagttacc tcgacgaagt ggtgctggat gacgccggaa accgcatgtt
     7621 tgtctgctcc gataccgatt attgccgcca acagaacgag gccaaaagcc aatgaatcaa
     7681 ccgttacttt cggtcaataa cctgacccac ctttatgccc cgggcaaagg cttcagcgat
     7741 gtctcttttg atttatggcc gggagaagtt ctgggcattg ttggggagtc cggttccggg
     7801 aagaccacgc tactgaaatc aatttccgcc cgcctgacac cacagcaagg ggaaattcgc
     7861 tacgagaacc gttcgctgta cgcgatgagc gaggccgacc gccgtcgcct gctgcgcacc
     7921 gaatggggtg tggtgcatca gcatccgctt gacggcctgc gccgccaggt gtcggcaggc
     7981 ggcaatatcg gcgaacggtt gatggcaacc ggagcgcgtc attacggcga tattcgcgcc
     8041 accgcgcaga aatggctgga agaggtggag attcccgcca accggatcga cgacctgccg
     8101 accacctttt ccggcggcat gcagcaacgt ttgcagattg cccgcaacct ggtgacgcat
     8161 ccgaagctgg tgtttatgga tgaacccact ggcgggctgg atgtgtcggt acaggcgcgc
     8221 ctgctggacc tgctccgcgg tctggtgatg gagcttaacc tggcggtggt gattgtcacc
     8281 cacgatttag gcgtcgcccg tctgctggcg gaccgtttgc tggtaatgaa gcaagggaaa
     8341 gtggtggaga gtgggttaac cgaccgggtg ctcgacgacc cgcaccatcc gtatacccag
     8401 ttgctggtgt catcggtttt gcagaattga gctggtgccg gatgcggcgt aaacgcctta
     8461 tccggcctac aaatgcgctc cccgtaggtc ggataagacg catcagcgtc gcatccgaca
     8521 cccgaaccac gaggcgaaaa atgattaacg tacaaaacgt cagtaaaacc ttcatcctgc
     8581 accagcaaaa cggcgtgcgc ctgcctgtat tacaaggcgc ctcactcacc gtcaacgccg
     8641 gcgaatgcgt ggtactccac ggccattccg gcagtggaaa atcaactctg ctacgctcgc
     8701 tgtatgccaa ctatctaccc gacgaagggc aaatccagat caaacacggt gatgaatggg
     8761 tcgacctggt caccgcgcca gcgcgcaaag tggtggagat ccgcaaaacc actgtcggct
     8821 gggtaagcca gtttctgcgc gtcatcccac gtatttcggc actggaagtg gtgatgcagc
     8881 cgctgctcga taccggcgta ccgcgtgaag cctgcgccgc taaagccgcg cgtctcctca
     8941 cccgcctgaa cgtgcctgaa cgcctgtggc accttgcgcc atcgaccttc tccggtggcg
     9001 aacagcagcg tgtcaacatc gcccgtggct ttatcgtcga ctaccccatt ctgctgctgg
     9061 atgaacccac cgcctcgctg gacgctaaaa acagcgccgc cgtagtggaa ctgatccgcg
     9121 aagccaaagc ccgcggcacg gccatcgtcg gtatcttcca tgacgaaacc gtgcgcgagc
     9181 aagtcgccga ccgcctgcac ccaatgggag tctcttcatg attatcaata acgtcaaact
     9241 ggtgctggaa aacgaggtgg tacacggttc tctggagatg caggacggcg aaatccgcgc
     9301 ctttgccgaa agccagagtc acctgtcaga agcaatggac ggcgaaggcg gctggctgct
     9361 accggggctg attgagctgc ataccgataa tctggataaa ttcttcaccc cgcgcccgaa
     9421 agttgactgg cctgcccact cggcgatgag cagccacgac gcgctgatgg tggcgagcgg
     9481 catcaccacc gtgctggacg ccgtggcgat tggtgatgta cgcgacggcg gcgatcggct
     9541 ggagaatctg gagaagatga tcaacgccat cgaagagacg cagaaacgcg gcgtcaaccg
     9601 tgccgagcac cgtctgcacc tacgctgcga actgccgcat cacaccacgc tgccgctgtt
     9661 tgaaaaactg gtacagcgcg aaccggtaac gctggtgtcg ctgatggatc actcaccggg
     9721 ccagcgccag ttcgccaacc gcgaaaaata tcgtgaatat tatcagggca aatactcgct
     9781 caccgatgcg cagatgcaac agtacgaaga ggagcaactg gcgctcgccg cacgctggtc
     9841 acagccgaat cgcgaatcca tcgccgcaat gtgccgcgcg cgacacattg cgctcgccag
     9901 ccacgatgac gccacgcacg cccacgtagc ccaatcccat cagcttggca gcgtgatcgc
     9961 cgaatttcct accacattcg aagcggcaga agcctcgcgc aagcacggca tgaacgtact
    10021 gatgggcgcg ccgaatatcg tgcgcggcgg ttcgcactcc ggcaacgtgg cggccagtga
    10081 actggcgcag cttggcctgc tggatatcct ctcttccgac tactaccccg ccagcctgct
    10141 ggatgcggcg tttcgcgtgg ccgatgacca gagcaaccgc tttacgctgc cgcaggcggt
    10201 gaggctggtg accaaaaacc cggcccaggc actgaatctc caggatcgtg gggtgattgg
    10261 cgagggcaaa cgcgccgacc tggtgctggc gcatcgcaag ggcaatcaca ttcatattga
    10321 ccacgtctgg cgtcagggta aaagggtgtt ctgatgacgg gaaaactgat ttggttaatg
    10381 ggtccgtccg gctccgggaa agacagcctg ctggcggaac tccgccagcg ggaacaaacc
    10441 cagctactgg tagcgcatcg ctacatcacg cgtgatgcca gcgccggaag tgaaaatcat
    10501 atcgccttaa gcgagcggga gttttttacc cgcgcggggc aaaatctgtt ggccttgagc
    10561 tggcatgcta atggtttgta ttacggtgtc ggcattgaga tcgacctctg gctgcacgcc
    10621 ggattcgacg tcgtggttaa cggctcacgc gcccacctgc cgcaggcgcg ggcgcgttat
    10681 caatcggcgc tgctgcccat ttgcttacag gtttcgccgg agatcctgcg ccagcgcctg
    10741 gaaaaccgtg gccgtgaaaa cgccagtgaa attaacgccc ggctggcgcg cgccgcccgc
    10801 tatactcctc aggattgcca tacgctcaat aatgacggca gcctgcgcca gtcggtcgac
    10861 acgctgctga cgctgattca tcagaaggag aaacaccatg cctgcttgtg aacttcgccc
    10921 ggcaacgcag tacgacaccg acgcggttta cgcgctgatt tgtgagctaa aacaagcgga
    10981 gtttgaccac caggcgtttc gcgtgggctt taacgccaac ctgcgtgacc cgaatatgcg
    11041 ctaccatctg gcgctgttgg atggtgaggt tgtcggcatg atcggcctgc atttgcagtt
    11101 tcatctgcat catgtcaact ggatcggcga aattcaggaa ctggtggtga tgccgcaggc
    11161 gcgcggtctg aacgtcggca gtaaattact ggcatgggca gaagaagaag cccgccaggc
    11221 cggggccgaa atgaccgagc tttccaccaa cgtgaagcgc cacgacgcac accgttttta
    11281 cctgcgtgaa ggctacgagc agagccactt ccgcttcacc aaggcgctgt aatatgagcc
    11341 tgaccctcac gctcaccggc accggcggcg cacagggcgt tccggcatgg ggctgcgagt
    11401 gcgtagcctg cgccagagcg cggcgctcac cgcagtatcg ccgccagccg tgcagcggcg
    11461 tagtgaagtt taacgaggcg atcaccctga tcgacgccgg gctgcacgat ctcgccgatc
    11521 gctggtcgcc cggatcgttc cagcagtttt tgctgacgca ctaccatatg gatcacgtcc
    11581 aggggctgtt tccgctgcgc tggggcgtgg gcaatcagat cccggtctac ggcccgccgg
    11641 atgaacaggg ctgcgacgat ctgtttaaac atccgggcct gcttgatttc agccacacgg
    11701 tggagccgtt tgtggtgttt gatttgcagg ggttgcaggt cacgccgctg ccgcttaacc
    11761 attcgaaact cactttcggc tatttactgg aaacggcgca cagccgggtg gcgtggctat
    11821 ctgacaccgc tggcctgccg gaaaaaacgc tgaaattttt acgcaataat cagccgcagg
    11881 taatggtgat tgattgcagc cacccgccac gcgaggatgc accgcgtaat cactgtgatt
    11941 taaataccgt gcttgcgctg aatcaggtta tccgctcgcc acgggtgatt ctgacccata
    12001 tcagccacca gtttgatgcg tggctgatgg aaaacgcact gccgtcgggg tttgaggcgg
    12061 gatttgatgg gatggagatt gaggtggagt gagggaaaat acccgcgccg gcccctacct
    12121 caacgcccat actcatcttc cattctccgc tcttcatctt ccagttgccg acgctcctga
    12181 tcaagctggc gctggcgatc gtccagctgc ctgcggcgat cttcaaactg gcggcggcgg
    12241 tcatcatatt gtctgcgccg atcgtcgctc acttcacgct gccagccgtc gtcgcgcgaa
    12301 tcttcatagt ctcgcccacg gtcagggtta taagcgtcgt taatcgcctg ctgaatattg
    12361 ccaatggtgt cgtcgataat atcggcctgg gccggaacgg tggacagcgt gagcagggtg
    12421 aataaaagaa atagcggaaa gcgtttcatt agccaacctc aaaaagaaac tctatccaca
    12481 ttagtcatta ctcatccatg caagtagtgg atgaatctca attcctttgc tgctctattg
    12541 ccgtaatcgc ctccacgcgt tgttgatgac gaccgccttc gtactgtgcg cccagccacg
    12601 catcgacaat catttttgcc agttcgaggc caaccactcg tgaaccaaaa gccagcacgt
    12661 tggtgtcgtt atgctgccgc gaaagttgcg cggaataagg ttcgctacaa acgaccgcgc
    12721 gaattccggc aaacttgttc gccgctatcg aaataccgac gccagtgcca caaatcaaaa
    12781 tcccgccatc agcctctccg ccagcaacgg ccagtgcgac ttggctggcg taatgcggat
    12841 aatcagtacg ctctgacgac caggttcctt tatcaatcac ttcaacgtca cgctcaacta
    12901 aatgtgccac tatttcatgt tttaaaatga aaccgacatg atcacagcca aatgcaatct
    12961 ttttcattca tcttccccgc ccgtgcaaaa cttcacaatc aaaaaacatt tgtgatgtaa
    13021 atgaattaaa aacacccaaa atcgatgaaa tccgccttcc cgatctgttt tacagagtta
    13081 catctggcag cgcaatagcg ttaaagacac tcactatcca gcaacatcta tcatctaata
    13141 aaccaggaaa acaaataaca tcatgttttt aaagtgatta aatgaaacga aattttaagc
    13201 tactctccat tattcacaac aaaacaaact ttataaattt tatttttttg tgaagttacc
    13261 agcatctttt ctgttcttgc tgtggtgata tagtggcgtc ttcaattcaa ggacaagaga
    13321 acgtgatgag ccagtcagag tttgattcag cgcttccgaa cggtatcggg ttagcgcctt
    13381 acctgcgaat gaagcaggaa ggaatgacag aaaacgaaag ccgcatcgtg gagtggttac
    13441 tcaaacccgg taacctgagt tgtgcacccg caattaaaga tgtcgcagaa gctctggcgg
    13501 tatctgaagc gatgatcgtt aaggtatcaa agctgctggg gtttagcggc tttcgtaact
    13561 tacgcagtgc gctggaagat tatttttctc agtcagaaca agtattgcct tccgagttgg
    13621 cttttgatga agcgccgcag gatgtggtga ataaggtatt taacatcact ttacgcacca
    13681 ttatggaagg tcagtcgatc gtcaacgttg atgagatcca ccgtgccgcc cgctttttct
    13741 atcaggccag acagcgggat ttgtacggtg ccggaggatc aaatgctatc tgtgctgatg
    13801 tacagcacaa gtttttgcgc attggcgtac gctgccaggc ctatccggac gctcacatca
    13861 tgatgatgtc tgcttcgttg ttacaggaag gagatgtggt gctggtggtg acccattccg
    13921 ggcgaaccag tgatgtaaaa gcggctgtag aactggcaaa aaagaacggg gcaaagatta
    13981 tttgtataac gcatagctat cattcaccaa tagcgaagct ggccgattat attatttgct
    14041 caccggcccc ggaaacaccg ttattaggtc gtaatgcctc ggcaagaata ttacaactga
    14101 ctttgctgga cgcgtttttt gtctctgtcg cccagctcaa cattgaacaa gctaatatta
    14161 atatgcaaaa aaccggcgca attgttgatt tcttctcacc aggcgcgctg aaataagaaa
    14221 aaggtaattc cgccatcgcg gagttacctc tccccttacc gtgagaacat gattatgaat
    14281 aaatatctga aatatttcag cggcacactc gtgggcttaa tgttgtcaac cagcgctttt
    14341 gctgccgccg aatatgcagt cgtattgaaa acactctcca atccattttg ggtagatatg
    14401 aaaaaaggca ttgaagatga agcaaaaacg ctgggcgtca gcgttgatat ttttgcctct
    14461 ccttcagaag gcgattttca atctcaattg cagttatttg aagatctcag taataaaaat
    14521 tacaaaggta tcgccttcgc accattatcc tcagtgaacc tggtcatgcc tgtcgcccgc
    14581 gcatggaaaa aaggcattta tctggtcaat ctcgatgaaa aaatcgacat ggataatctg
    14641 aaaaaagcag gcggtaatgt ggaaggtttt gtcaccaccg ataatgttgc cgtcggggcg
    14701 aaaggcgcgt cattcattat tgacaagctg ggtgccgaag ggggtgaagt cgcaatcatt
    14761 gagggtaaag ccggtaacgc ctccggtgaa gcgcgtcgta atggtgccac cgaagccttc
    14821 aaaaaagcaa gccagatcaa gcttgtcgcc agccagcctg ccgactggga ccgtattaaa
    14881 gcactggatg tcgccactaa cgtgttgcaa cgtaatccga atattaaagc gatctattgc
    14941 gcgaatgaca cgatggcaat gggtgttgct caggcagtcg caaacgctgg aaaaacgggc
    15001 aaagtgttgg tcgtcggtac agatggcatt ccggaagccc gcaaaatggt ggaagccgga
    15061 caaatgaccg cgacggttgc ccagaacccg gcggatatcg gtgcaacggg tctgaagctg
    15121 atggttgacg ctgagaaatc cggcaaggtt atcccgctgg ataaagcacc ggaatttaaa
    15181 ctggtcgatt caatcctggt cactcaataa aactgaatga tgccggatga tgatgctaat
    15241 gaagtgtctt atccggccaa caaaataacc tgaacgtaga cctgataaac gtagccatca
    15301 ggcaataata cgatatttcg tgcagagagg ttatttatgg ccacgccata tatatcgatg
    15361 gcggggatcg gcaagtcctt tggtccggtt cacgcattaa agtcggttaa tttaacggtt
    15421 tatcctggtg aaatacatgc attactagga gaaaatggtg cgggtaaatc tacgctaatg
    15481 aaagtgttat ccgggataca tgagccgacc aaaggcacca ttaccattaa taacattaac
    15541 tataacaaac tggatcataa attagcggca caactcggta tcgggattat ttatcaggaa
    15601 ctcagcgtta ttgatgaatt aaccgtactg gaaaatttat atattggtcg tcatctgacg
    15661 aaaaaaatct gcggtgtcaa tattatcgac tggcgagaaa tgcgtgtccg cgccgcaatg
    15721 atgttattac gcgtgggctt gaaagttgat ttagatgaga aagtggcgaa tttatctatc
    15781 agccacaagc agatgctgga aattgccaaa acgctgatgc tcgacgccaa agtcatcatc
    15841 atggatgaac ccacctcctc actcaccaat aaagaggtgg actatctgtt tctgatcatg
    15901 aatcagttgc gtaaggaggg tacggccatc gtctatatct cgcataaatt ggcggaaatt
    15961 cgccgtattt gcgaccgcta tacggtgatg aaagacggca gcagcgtttg cagcggcatg
    16021 gtgagcgatg tgtcaaatga cgatatcgtc cgtctgatgg taggccgcga attgcaaaac
    16081 cgttttaacg cgatgaaaga gaatgtcagc aaccttgcgc acgatacggt ttttgaggtg
    16141 cgcaacgtca ccagccgtga cagaaaaaag gtccgggata tctcatttag cgtctgccgg
    16201 ggagaaatat taggctttgc cggactggtc ggttccggac gtactgaact gatgaactgc
    16261 ctgtttggcg ttgataaacg cgctggcgga gaaatccgtc ttaatggcaa agatatctct
    16321 ccacgttctc ccctggatgc cgtgaaaaaa gggatggctt acatcactga aagccgccgg
    16381 gataacggct ttttccccaa cttttctatc gctcagaaca tagctatcag ccgcagtctg
    16441 aaagacggcg gctataaagg cgcgatgggg ttgtttcatg aagttgacga gcaacgtacc
    16501 gctgaaaatc aacgcgaact gctggcgctg aaatgtcatt cggtaaacca gaacatcact
    16561 gaactctccg gcggtaacca gcagaaagtc ctgatctcca aatggctgtg ctgtcgcccg
    16621 gaagtgatca ttttcgatga acctacccgt ggcatcgacg ttggcgcgaa agccgaaatt
    16681 tacaaagtga tgcgccaact ggcggacgac ggaaaagtca tcctgatggt gtcatctgaa
    16741 ctacctgaaa ttatcaccgt ctgcgaccgc atcgccgtgt tctgcgaagg acgactgacg
    16801 caaatcctga cgaatcgcga tgacatgagc gaagaggaga ttatggcatg ggctttacca
    16861 caagagtaaa aagcgaagcg agcgagaaga aaccgttcaa ctttgcgctg ttctgggata
    16921 aatacggcac cttttttatc ctggcgatca tcgtcgccat ttttggttcg ctgtcgtcag
    16981 aatattttct gaccaccaat aatattaccc agatttttgt gcaaagctcc gtgacggtac
    17041 tgatcggcat gggggaattc ttcgctatcc tggtcgccgg tatcgacctt tcggttggcg
    17101 cgattctggc gctttccggt atggtgaccg ctaaactgat gttggcaggt attgatccgt
    17161 ttctcgcggc gctaattggc ggtgtactgg ttggcggcgc actgggggcg atcaacggct
    17221 gcctggtcaa ctggacggga ctacacccgt tcattatcac ccttggcacc aacgctattt
    17281 tccgtgggat cacgctggtg atctccgatg ccaactcggt atacggcttc tcatttgact
    17341 tcgtgaactt ctttgccgcc agcgtaattg ggatacctgt ccccgttatc ttctcgctaa
    17401 ttgtcgcgct catcctttgg tttctgacaa cgcgtatgcg gctcgggcgc aacatctacg
    17461 cactgggcgg caacaaaaac tcggcgttct attccgggat tgacgtgaaa ttccacatcc
    17521 tggtggtgtt tatcatctcc ggtgtttgtg caggtctggc aggcgtcgtc tcaactgcac
    17581 gactcggtgc cgcagaaccg cttgccggta tgggttttga aacctatgcc attgccagcg
    17641 ccatcattgg cggcaccagt ttcttcggcg gcaaggggcg cattttctct gtggtgattg
    17701 gcgggttgat catcggcacc atcaacaacg gtctgaatat tttgcaggta caaacctatt
    17761 accaactggt ggtgatgggc ggattaatta tcgcggctgt cgcccttgac cgtcttatca
    17821 gtaagtaagg aattgaacat gaaaatctcc ccctcgttaa tgtgtatgga tctgctgaaa
    17881 tttaaagaac agatcgaatt tatcgacagc catgccgatt actttcacat cgatatcatg
    17941 gacggccact ttgtgccgaa tctgacgctc tcaccgttct tcgtaagcca ggtaaaaaaa
    18001 ctggcgagta aaccgctcga ctgccatctg atggtaacgc gtccgcagga ttacattgct
    18061 caactggcac gtgcgggagc tgatttcatc actttgcatc cggaaactat caacggtcag
    18121 gcattccgcc tgattgatga aatccgccgt catggcatga aagtggggct gattcttaat
    18181 ccggaaacgc cggttgaggc catgaaatac tatatccata aggctgataa aattacggtc
    18241 atgactgtcg atcccggttt tgccggacaa cccttcattc ctgaaatgct ggataaagtt
    18301 gccgaactga aggcatggcg tgaacgagaa ggtctggagt acgaaattga ggtggacggt
    18361 tcctgcaacc aggcaactta cgaaaaacta atggcggcag gggcggatgt ctttatcgtc
    18421 ggcacttccg gcctgtttaa tcatgcggaa aatatcgacg aagcatggag aattatgacc
    18481 gcgcagattc tggctgcaaa aagcgaggta cagcctcatg caaaaacagc ataacgtcgt
    18541 agcgggcgtg gatatggggg caacgcatat ccgcttttgt ctgcggacag cagaaggtga
    18601 aacgctacac tgcgaaaaaa agcagaccgc agaagttatt gctcccgacc tggtgtcggg
    18661 tatcggcgaa atgattgacg agcaactcag gcgctttaac gctcgctgtc atggtctggt
    18721 gatgggattt ccggcgctgg tcagtaaaga taaacgcacc attatttcta cgcctaacct
    18781 gccgttaaca gcggcagatt tatatgatct cgccgataag ctcgaaaata cgctgaattg
    18841 tccggttgag ttttcccgcg acgttaacct gcaactctcc tgggacgtag tagaaaatcg
    18901 ccttacgcaa caactggttc tggcagccta tctcggtacg gggatggggt tcgcagtgtg
    18961 gatgaacggt gcgccgtgga cgggtgcaca cggtgtggca ggcgaactgg gccatatccc
    19021 cctaggagat atgacccaac actgcgcgtg tggtaatcct gggtgcctgg aaaccaattg
    19081 ctctggaatg gcgctaagac gctggtacga acaacagccc cgaaattacc cattgagcga
    19141 tcttttcgtc catgcggaaa acgccccttt cgtccagagt ctgcttgaaa acgcggcacg
    19201 agccattgcc accagcatta atctgttcga tcccgatggg gtgattctgg gtggtggcgt
    19261 gatggatatg cccgccttcc cacgcgagac tctcattgcc atgacccaaa agtacctgcg
    19321 ccgtccactg ccgtatcagg tcgtgcgctt tattgccgcc tcatcttctg actttaatgg
    19381 cgctcagggt gcagcaatat tggcgcatca acgttttttg ccacagtcct gtgctaaagt
    19441 cccatgagta aagcgttggc acagtagcgc aatgtcccgc tgtgccaacg acaggtacaa
    19501 tatatataat aattacataa aacagccata ttattcccag caatgatagt tctactgtaa
    19561 ttttccctct tcagcacaaa tgcctaataa aatggctcgt tccgctcaca cttccactta
    19621 ttaaagtcgc aaattcccaa ttatgataac gccagcttta aatgacgtat tttgcatgga
    19681 ttacatattt acgacataaa tgataaatgg agatttatat gaataactct cggttattcc
    19741 gtttgagcag gattgttatt gcgttcactg ccgccagcgg catgatgata aataccgctt
    19801 acgcaacaga tgaggcgaaa gccgccactc aatataccca acaggttaat cagaattacg
    19861 ccaaatcatt accgtttggc gatcgccagg attttgacga tgctcagcgt ggatttatcg
    19921 ctccgctgct ggatgaaggt attctgcgtg atgcgaacgg taaaatttac taccgcgcga
    19981 atgattacaa atttgatatt aatgccgctg cgccggaaac cgtaaacccc agcctgtggc
    20041 gtcagtcgca gatcaacggc atttctggcc tgttcaaagt caccgataaa atgtatcagg
    20101 tgcgcggcca ggatatctct aacattacgt tcgttgaggg cgagaaaggc attattgtta
    20161 ttgacccact ggtgacgcca cctgccgcaa aagccgcact tgacctttac ttccagcatc
    20221 gtccgcaaaa accgattgtt gccgttatct acactcacag ccacgccgac cactacggcg
    20281 gcgtgaaagg tattatctct gaagccgatg ttaaatccgg caaagttcag gtgattgctc
    20341 cagctggctt tatggacgaa gccatcagcg aaaacgtgct ggcgggtaac atcatgagcc
    20401 gccgtgcgct ctactcttac ggtctgttac tgccgcacaa cgcgcaaggc aatgtgggta
    20461 atggccttgg cgtgacgctg gcaacgggcg acccgagcat tattgcaccg actaaaacta
    20521 tcgtcagaac tggcgagaag atgattatcg acggcctgga gtttgacttc ctgatgaccc
    20581 caggtagcga agcgccagcg gaaatgcact tctatattcc ggccctgaaa gccctgtgta
    20641 ccgccgagaa tgccacgcat accctgcaca acttctacac cctgcgcggc gcgaaaaccc
    20701 gcgacaccag caagtggacc gagtatctga acgaaacatt ggatatgtgg ggtaacgacg
    20761 cggaagtact gtttatgccg cacacctggc cggtctgggg caataagcat atcaatgatt
    20821 atattggtaa atatcgcgat actatcaagt acattcacga ccagaccctg cacctggcga
    20881 accagggcta caccatgaat gaaatcggcg acatgattaa gctgccgcct gcgcttgcca
    20941 ataactgggc cagccgcggc tattacggtt ctgtcagcca caacgcccgc gcggtatata
    21001 acttctatct tggctattac gacggtaacc cggctaacct gcatccgtat ggtcaggtgg
    21061 agatgggtaa acgttacgtg caggcgctgg gcggttctgc ccgcgtcatc aacctggcgc
    21121 aagaagcgaa caagcaaggt gattatcgct ggtcggcaga actgctgaaa caggtgattg
    21181 ccgccaaccc gggtgaccag gtcgcgaaga atctgcaagc gaataacttt gaacagctgg
    21241 gctatcaggc cgagtccgcc acatggcgcg gtttctacct gaccggcgcg aaagagctgc
    21301 gcgaaggggt gcataagttc agccacggca ccaccggttc cccggacacc attcgtggga
    21361 tgtcggtcga aatgctgttc gactttatgg ccgttcgcct cgatagcacg aaagccgcag
    21421 gtaaaaatat cagcctgaac ttcaatatga gcaacggcga taacctcaac ctgatgctga
    21481 acgatagcgt gcttaactac cgtaaaacgc tgcaatcgca agccgacgcc tctttctaca
    21541 ttagccgcga agatctgcac gccgtgctga ccggacaggc taaaatggcc gatctggtga
    21601 aagcgaagaa agccaaaatt attggcaatg gcgcgaaact ggaagaaatt atcgcctgtc
    21661 tggataattt cgatttgtgg gtgaatatcg taaccccaaa ttaatgtcac tcgct
//
LOCUS       DAZGJT010000080        21059 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZGJT010000080 DAZGJT010000000
VERSION     DAZGJT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21059
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.080"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(160..822)
                     /gene="fsa"
                     /locus_tag="ACHKFK_003335"
     CDS             complement(160..822)
                     /gene="fsa"
                     /locus_tag="ACHKFK_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO9053604.1"
                     /translation="MELYLDTSDVVAVTALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            952..1851
                     /gene="ybiY"
                     /locus_tag="ACHKFK_003336"
     CDS             952..1851
                     /gene="ybiY"
                     /locus_tag="ACHKFK_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO9053605.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDPRLCLEGCKLCAKTAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVSEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            1857..4289
                     /gene="ybiW"
                     /locus_tag="ACHKFK_003337"
     CDS             1857..4289
                     /gene="ybiW"
                     /locus_tag="ACHKFK_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO9053606.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSHINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPIGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            4435..5250
                     /gene="ybiV"
                     /locus_tag="ACHKFK_003338"
     CDS             4435..5250
                     /gene="ybiV"
                     /locus_tag="ACHKFK_003338"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO9053607.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMSNAAENIKQIARYATDDNNHEGALNVIQTVLENKPPFNL"
     gene            complement(5450..7042)
                     /gene="ybiT"
                     /locus_tag="ACHKFK_003339"
     CDS             complement(5450..7042)
                     /gene="ybiT"
                     /locus_tag="ACHKFK_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO9053608.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            7261..8181
                     /gene="ldtB"
                     /locus_tag="ACHKFK_003340"
     CDS             7261..8181
                     /gene="ldtB"
                     /locus_tag="ACHKFK_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9053609.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(8239..9357)
                     /gene="ybiR"
                     /locus_tag="ACHKFK_003341"
     CDS             complement(8239..9357)
                     /gene="ybiR"
                     /locus_tag="ACHKFK_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO9053610.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     LKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPVLQGVLSNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAG"
     gene            complement(9354..9821)
                     /gene="mntR"
                     /locus_tag="ACHKFK_003342"
     CDS             complement(9354..9821)
                     /gene="mntR"
                     /locus_tag="ACHKFK_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO9053611.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            10007..10135
                     /gene="mntS"
                     /locus_tag="ACHKFK_003343"
     CDS             10007..10135
                     /gene="mntS"
                     /locus_tag="ACHKFK_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO9053612.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            10407..11990
                     /gene="opgE"
                     /locus_tag="ACHKFK_003344"
     CDS             10407..11990
                     /gene="opgE"
                     /locus_tag="ACHKFK_003344"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO9053613.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGIFIFALGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(12040..12555)
                     /gene="ompX"
                     /locus_tag="ACHKFK_003345"
     CDS             complement(12040..12555)
                     /gene="ompX"
                     /locus_tag="ACHKFK_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO9053614.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(12608..12673)
                     /locus_tag="ACHKFK_003346"
     CDS             complement(12608..12673)
                     /locus_tag="ACHKFK_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053615.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            12908..13795
                     /gene="rhtA"
                     /locus_tag="ACHKFK_003347"
     CDS             12908..13795
                     /gene="rhtA"
                     /locus_tag="ACHKFK_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO9053616.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGTLQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            14095..14598
                     /gene="dps"
                     /locus_tag="ACHKFK_003348"
                     /gene_synonym="pexB"
     CDS             14095..14598
                     /gene="dps"
                     /locus_tag="ACHKFK_003348"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO9053617.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            15002..15748
                     /gene="glnH"
                     /locus_tag="ACHKFK_003349"
     CDS             15002..15748
                     /gene="glnH"
                     /locus_tag="ACHKFK_003349"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO9053618.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            15887..16546
                     /gene="glnP"
                     /locus_tag="ACHKFK_003350"
     CDS             15887..16546
                     /gene="glnP"
                     /locus_tag="ACHKFK_003350"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO9053619.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            16543..17265
                     /gene="glnQ"
                     /locus_tag="ACHKFK_003351"
     CDS             16543..17265
                     /gene="glnQ"
                     /locus_tag="ACHKFK_003351"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO9053620.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            17382..19607
                     /gene="ybiO"
                     /locus_tag="ACHKFK_003352"
     CDS             17382..19607
                     /gene="ybiO"
                     /locus_tag="ACHKFK_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO9053621.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGITTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(19604..20530)
                     /gene="rlmF"
                     /locus_tag="ACHKFK_003353"
     CDS             complement(19604..20530)
                     /gene="rlmF"
                     /locus_tag="ACHKFK_003353"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO9053622.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            20806..20985
                     /locus_tag="ACHKFK_003354"
                     /pseudo
     CDS             20806..20985
                     /locus_tag="ACHKFK_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            complement(21002..21058)
                     /locus_tag="ACHKFK_003355"
     CDS             complement(21002..21058)
                     /locus_tag="ACHKFK_003355"
                     /inference="COORDINATES: protein motif:HMM:NF015365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9053623.1"
                     /translation="MELHDKVIGHYLNIKHYQ"
BASE COUNT         5162 a         5523 c         5183 g         5191 t
ORIGIN      
        1 ttaagcgtcg catccggcat ttgctccaga cgccagatgc ggcgtgaacg ccttatctgg
       61 cctacggttc tgacgcagat ttccaggcct gatagacgcg gcaggcatcg gtgcactccg
      121 ccacgtcatc agcaatttat gaggatgcag aacgtccggt taaatcgacg ttctgccaaa
      181 cgctcccagc cagtcctgtt caaacttcgc cacagcggca tcaaccgccg gatagctaat
      241 catctgttgt gccacatcca gcggcagagt aattgattca catcctgcca gtaagcagtc
      301 cagcgcctga cgcggggttt tgaaactcgc ggctaacact ttcgcctgcg gcgcatgcat
      361 tttcaataac tggtgtaagt cggtcacagt ctgaataccg ctaccgccct gagcatcaat
      421 acgattaacg taaggcgcaa catattccgc acctgccagc gccgacagta gcccttgtgc
      481 tgcgccatat accgcggttc ccagcgtcgg aatcccttcc gcttttaaca tcttaatagc
      541 tgccagcccc tcagcggtca ccggaacttt caccacgata tccgcaataa tagaacgcaa
      601 tttacgtgcg tcattaacca tcccttcagc agtggtagcc attacctggg caaacagacg
      661 cccctgaccg cccatcgcct cgtgaagttg cggaagcaca acctccagcg gttttttacc
      721 cgctgcgata atgcttgggt tagtggtcac acccgccagc ggaaaaatac gtgacagcgc
      781 cgtcaccgca acaacgtctg aagtatccag atacagttcc ataaccatcc tcaaaatgta
      841 ctttttgtct gaatgtagac tatcacggga aaactctctg aacagttgac gcacatcaat
      901 ctaacttcat tcgaaagtat tttaatgttt gaacgaaaca gagaggcaac tatgattttc
      961 aatattcaac gctactcgac ccatgatggc cccggtatcc gcacggtcgt atttcttaaa
     1021 ggctgttcgc tgggctgccg ctggtgtcag aacccggaaa gccgcgcccg cacgcaggat
     1081 ctgctgtatg acccacgact ctgtctggaa ggctgtaagc tgtgcgctaa gaccgcaccg
     1141 gaagtgattg agcgcgcgtt gaatggtttg cttattcatc gggaaaagtt aaccccggag
     1201 cattttacgg cgctaaccga ctgctgcccg acacaggcgt taaccgtgtg tggtgaagtg
     1261 aaaagcgttg aggagatcat ggcaaccgtt ttgcgcgata aaccgtttta cgatcgcagc
     1321 ggcggcggct taacgctttc tggcggtgaa ccgtttatgc agccggaaat ggcgacggcg
     1381 ctgctgcaag ccagccacga ggccggcatt catactgcgg tagaaacctg tctgcatgtg
     1441 ccgtggaaat atatcgcccc ttctctgccc tatatcgatc tgtttcttgc cgatttaaaa
     1501 cacgttgccg acgcgccgtt taaacagtgg accgacggta acgccgccag ggtgctggat
     1561 aacctgaaaa aactcgccgc agccggcaaa aaaatcatta tccgcgtgcc actgattcag
     1621 ggctttaatg ccgacgaaac ttctgtgaaa gccattaccg attttgccgc cgacgagctg
     1681 cacgttagcg aaattcattt tctgccctac cacacactgg gcatcaacaa atatcactta
     1741 cttaatctgc cctatgacgc cccggaaaaa ccgcttgatg cgccagaact gctcgacttt
     1801 gcccagcagt atgcctgcca gaaagggtta accgcgacct tacgaggata acaatcatga
     1861 caacactaaa actggatacg ctcagcgacc gcattaaagc gcacaaaaat gcgctggtgc
     1921 atattgtgaa accgccggtc tgtaccgaac gagcacaaca ctataccgag atgtatcaac
     1981 aacatctcga taagccaatc ccggtacgtc gcgcgctggc actggcgcat cacctggcga
     2041 atcgcaccat ctggatcaag cacgatgagt tgatcattgg caaccaggca agcgaagttc
     2101 gcgccgcgcc gatcttcccg gaatataccg tctcgtggat cgaaaaagag attgatgatc
     2161 tggcagatcg tcccggtgct ggttttgcgg tgagcgaaga gaacaaacgc gttctgcatg
     2221 aagtgtgccc gtggtggcgc ggtcagaccg tacaggatcg ctgctacggc atgtttaccg
     2281 atgagcaaaa aggtctgctg gcgaccggaa tcattaaagc ggagggcaat atgacctccg
     2341 gcgatgcgca cctggcggtc aatttcccgc tactgctgga aaaaggtctt gatggtctgc
     2401 gtgagaaagt ggcggaacgt cgctcgcaca tcaatctgac ggttctggaa gatctgcacg
     2461 gcgagcaatt cctgaaagcg attgatatcg tgctggtggc agtcagtgaa cacattgaac
     2521 gtttcgctgc cctggcgcgt gaaatggccg cgaccgaaac ccgcgaaagc cgtcgcgatg
     2581 aactgctgac gatagcagaa aactgcgatc ttatcgccca ccagccgccg cagactttct
     2641 ggcaggcgct gcaactatgt tacttcatcc agttgatttt gcagatcgaa tctaacggtc
     2701 actcggtatc gtttggtcgt atggaccagt atctctatcc atactatcgc cgtgacgttg
     2761 aactgaacca gacactggat cgcgaacacg ccatcgagat gctgcatagc tgctggttga
     2821 aattgctgga agtgaacaaa atccgctccg gctcacactc aaaagcctct gcgggaagtc
     2881 cgctgtatca gaacgtcacc attggcgggc aaaatctggt tgatggtcaa ccgatggacg
     2941 cggtaaatcc actctcttat gcgatcctcg aatcttgcgg tcgcctgcgt tcgactcagc
     3001 ctaacctcag cgtgcgttac catgcgggta tgagtaacga tttcctcgac gcctgtgttc
     3061 aggtgatccg ctgcggcttc gggatgccgg cgttcaacaa cgacgaaatc gtcatcccgg
     3121 aatttattaa actcggtatt gaaccgcagg acgcttacga ctatgcagcg attggttgta
     3181 tcgaaaccgc cgtcggtggc aaatggggct atcgctgtac cggcatgagc tttatcaact
     3241 tcgcccgcgt gatgctggcg gcgctggaag gcggtcgtga tgccaccagc ggcaaagtgt
     3301 tcctgccaca agaaaaagcg ttgtcagcgg gtaacttcaa caacttcgat gaagtaatgg
     3361 acgcatggga tacgcaaatc cgttactaca cgcgtaaatc aatcgaaatc gaatatgtcg
     3421 tcgataccat gctggaagag aacgtacacg atattctctg ctcggcgctg gtcgatgact
     3481 gtattgagcg agcgaaaagt atcaagcaag gcggcgcgaa atatgactgg gtttctggcc
     3541 tgcaggtcgg cattgctaac ctcggcaaca gcctggcggc agtgaagaaa ctggtatttg
     3601 aacagggcgc gattggtcag caacagctgg ctgccgcact ggcggatgac ttcgacggcc
     3661 tgactcacga gcagctgcgt cagcggctga ttaacggtgc gccgaagtac ggcaacgacg
     3721 atgatactgt cgatacgctg ctggctcgcg cttatcagac ctatattgac gaactgaagc
     3781 agtaccataa cccgcgctac ggtcgtggtc cgattggcgg caactattat gcgggcacat
     3841 cgtctatctc cgctaacgta ccgtttggcg cgcagactat ggcaacgccg gacggacgta
     3901 aagcacatac cccgctggca gaaggcgcta gtccggcctc cggtactgac catctcggcc
     3961 ctactgcggt cattggctcg gtgggtaaac tgcctacggc cgcgattctc ggcggcgtgc
     4021 tgctcaacca gaaactgaat ccagcaacgc tggagaacga atctgacaag cagaaactga
     4081 tgatcctgct gcgcaccttc ttcgaggtgc ataaaggctg gcatattcag tacaacatcg
     4141 tttcccgcga aacgctgctg gaagcgaaaa aacatccgga tcagtaccgc gatctggtag
     4201 tgcgtgtcgc gggctattcc gccttcttca ccgcgctctc tccagacgct caggacgata
     4261 tcatcgcccg tactgaacat atgctgtaat ctcctcagcc cggcggcgtc gtcgccgggt
     4321 caaatcattc acatcatcaa ttccgctctc actttcattc gaaatataat ttgtgctctg
     4381 cgtcacattg ttattacact gttacggttt cattttcgag ccaggagcac acctatgagc
     4441 gtaaaagtta tcgtcacaga catggacggt acttttctta acgacgccaa aacgtacaac
     4501 caaccacgtt ttatggcgca gtatcaggaa cttaaaaagc gcggcattga gttcgttgtc
     4561 gccagcggta atcagtatta ccagcttatt tcgttctttc ctgagctaaa ggatgagatc
     4621 tcttttgtcg cggaaaacgg cgcactggtt tacgaacatg gcaagcagtt gttccacggc
     4681 gaactgaccc gacatgaatc gcggattgtt attggcgagt tgctaaaaga taagcaactc
     4741 aattttgtcg cctgcggtct gcaaagtgca tatgtcagcg aaaatgcccc cgaagcattc
     4801 gtcgcactga tggcaaaaca ctaccatcgc ctgaaacctg taaaagatta tcaggagatt
     4861 gacgacgtac tgttcaagtt ttcgctcaac ctgccggatg aacaaatccc gttagtgatc
     4921 gacaaactgc acatagcgct ggatggcatt atgaaacccg ttaccagtgg ttttggcttt
     4981 atcgacctga ttattcccgg tctacataaa gcaaacggta tttcgcggtt actgaaacgc
     5041 tgggatctgt cgccgcaaaa tgtggtagcg attggcgaca gcggtaacga tgcggagatg
     5101 ctgaaaatgg cgcgttattc ctttgcgatg agcaatgctg cggaaaacat taaacaaatc
     5161 gcccgttacg ctaccgatga taataatcat gaaggcgcgc tgaatgtgat tcagacggtg
     5221 ctggaaaata aaccgccgtt taatctttga ttatcgccaa ttcagtttat tgagaaagtg
     5281 catgtcatta tgtcggatac ggcgtaaccg ccttattcgg catacaaaat catgaaaatt
     5341 caatatattg cgatacacta ccaggcctga taagcgtagc gcaccaggca atcttgcgtt
     5401 tgtcatcagt ttcagtagcc ctgaccatat cagggctact gataataact tactcgatcc
     5461 ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt tccggggtga
     5521 tttccagaat acgggtcgcc agggagctta caaactcacg gtcgtgagaa acaaagatca
     5581 gcgtgccctg atacagctcc agcgccatgt tcagcgactc aatggattcc atatccaggt
     5641 ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac ttaccaaaca
     5701 gcatccgccc tttttcccca ccggaaagca ctttcgctgg ctttttgatg tcgtcctggc
     5761 tgaacagtaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct tcctgcttcc
     5821 actggctcat ccattcgaac acggtcagat cattttcgaa ctcatattca tggtcctggg
     5881 cgtagtaacc aatgcgcgcg ttctcagacc attttacggt gccgctgtcc ggttgcagat
     5941 cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc agtacagcca
     6001 gtttttcacc cacttccagc agcaggttga gatttttaaa cagtggaccg ttatcaaacc
     6061 ctttagtcag accttccact tccagcgcgt tacggaacag tttcttatcc tgttcaaaac
     6121 ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt ttatcaatct
     6181 ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag cggctaacga
     6241 aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcagccagc agacgttcac
     6301 gcgcctgagt cgccgccgtc atgtactcat cgtagttgcc cggataaacg cgcagctcgc
     6361 cgtaatccag atccgccatg tgggtacaaa ccatgttaag gaagtgacgg tcgtgcgaga
     6421 tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga atggtgtcga
     6481 tgtcgaggtt gttggtcggt tcgtcgagca ggagaatgtc cggatcagca aacagcgcct
     6541 gcgccagcag cacacgcagc ttccagccag gagcaacttc actcatcggg ccgtagtgct
     6601 gttccactgg aattcccacg ccaagcaaca gttcaccagc gcgagcttcc gcagagtaac
     6661 cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct tcttcactca
     6721 tttccggcaa agcatagatg cggtcacgtt cctgcttcac ttcccacaac tctttatgcc
     6781 ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc tgacgcagct
     6841 taccaatgcg ctcattggga tcgagggaaa cgttacccaa cgtcggctct aagtcgccgc
     6901 cgaggatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg ccgtaacggt
     6961 tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg aactgcatgg
     7021 tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa accgcgcatt
     7081 atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc aagccaaagg
     7141 caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa cttataatgc
     7201 gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat ctcgcttaac
     7261 atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg cttttgcagt
     7321 accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt tggtcagaat
     7381 caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc cgccgagtac
     7441 cagatgggac tttccaatat gatggaagcg aacccgggtg tggatacctt cctgccgaaa
     7501 ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt tcatgaaggc
     7561 atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac caacaccgtt
     7621 atcgtgctgc cgatcggcat tggtcagtta ggcaaagaca cgcctatcaa ctggaccacc
     7681 aaagttgagc gtaaaaaagc aggcccgacc tggacgccga ccgctaaaat gcacgcagag
     7741 taccgcgctg cgggcgaacc gcttccggcg gtcgttccgg ctggtccgga taacccgatg
     7801 gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa cgccaacttc
     7861 ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga catcaaattc
     7921 ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc ggtaaaagcg
     7981 accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc taccactgaa
     8041 gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt gcagacagtg
     8101 accggtcagc cagatgttga ccaggttgtt ctggatgaag cgatcaaaaa tcgttccggg
     8161 atgccggttc gtctgaatta atcttcaaaa cgtaaagcaa aggcggactc ataatccgcc
     8221 tttttaattt gacagacctt atcccgccgg gatcataacc aacaaaacat atcccaccag
     8281 cgccgcccac aacagcatcg gtatcgaata aagatgaaaa cgccaccaga tacggcgatc
     8341 gttcgccatc cgtaacgcaa tcaaatttgc cagcgatccg ggtaataaac caaagccccc
     8401 cacgtttacc gcccatgcca gtaataaaga cggtggcaca taattgagca gcaaaatggt
     8461 actcggcacg ttactgatca cctgcgataa accgatcgcc gttaaccata atccgggttc
     8521 ggataaatga ctcacgttac ttaacacccc ctgcaaaact ggcagttggg ttagtaaatg
     8581 gacgtcgata aacatcgcca taaacaccag cagcagcgtc cagtccacac tgagcaccac
     8641 acgacgcgcc agcagcgcaa agcccgccgc cacaatcacc agtccccaca gctcttgttt
     8701 taactccagc gccgtcagaa agacgatata cagccccaga caactccaca ccagccgcgg
     8761 tttccactcc ggcgtttgca cccccgtatg gtattgcagc gcctttccag ggaaacaaca
     8821 ccagcacaac agcaggagtg tcagcatcat tgcgccagcc agcggtgcca tttgggcaat
     8881 aaatccggca aacgaaagac cagaacgtcc ccagataaga atattttgcg ggttgccaat
     8941 tggcgtcagt agcgaaccag cgttgactgc cagcgcctca aaaataatca gccgattaac
     9001 cggaatctca cacagtcttt ttagcgtgat agtcagcgga acaactataa acagtgcgac
     9061 atcgttggtc agaaaggtag aaagcagcgc tgccgccagt accataaaca tcgccagccg
     9121 acgctccgta gcaaaacggc gcaccatttt gcgccccagc acatcaaaat aaccgcttaa
     9181 ctcgacacct ttggtcagca gcatcaggcc gcttaaggtg atgatggtgt gccagtcgat
     9241 agcagcaggc caggatttcg gtgcaaaggg aacgaaaaaa cttaatccga tgccaacaag
     9301 aattaataac tgaaaaaaac gatcgccttg cagcgtgcgt aaaaaaggca ggctcatttg
     9361 gcaccgtgtt tctgggtaaa caaacgaaag gcatccagcg tctcttcact aacatggtgc
     9421 tccatacctt ccgcgtcgcg acgggcgatt tccggactga cgcccaacac caacaagaaa
     9481 ttttcgacta tctgatgtcg ctcgcggctt tcctgcgcca gcttctctcc ttctgccgtt
     9541 aaaaacacgc cacgccaggg gatcatttca atcagcccca tggttgccag tcgcttaagc
     9601 attttagcca ccgtcggttg cgaaaccccc agacgagcag ccatgtccac ctgacgagct
     9661 tcccccactt ccctgatcaa gtcagaaatc agctcaacgt aatcatcaat cagctcgcgc
     9721 cgatgcgcct ctctgacctg gcggaaccct tcaacgtgct cttccacgtt cactaactgc
     9781 gtcacttttt ttgctgttgg cgtacctgcg cgacgactca ttgtgcttcc tcattatggt
     9841 gacgcataca tagcgttctc atttcgtgcg cacattgtaa accagagttg cgaaggtaca
     9901 aaaaattaac gttttagcaa tagctatata atatagcctg tgctatatct gtatgtaatg
     9961 caatcatccc tcaaggatcg atgggattag caagtcagga ggtcttatga atgagttcaa
    10021 gaggtgtatg cgcgtgttta gtcattctcc ctttaaagta cggttaatgc tgctctctat
    10081 gttgtgcgat atggtcaaca acaaaccgca gcaggataaa ccttccgata aatagcggcg
    10141 tcgcggtacg ccgcttcact cctgctttta tgcaggcaca acgcgttttg gtctgaaaaa
    10201 ccccactttt tgtcggattt gcaatcccct tcgcaaaaga tttgttcgtc ggtagttgac
    10261 ctgaacagca gctcgctcta tcttcttgca gccctgcgta tattgcggct cgcggatgcg
    10321 gacccctttc ccctcttcac gcactcttgc aggtattgac ccttgacgcc agggtaagca
    10381 catggcgttt gttacgatag tggcatatga atttaaccct caaagaatcg cttgttaccc
    10441 gtagccgggt atttagcccg tggactgcgt tctacttttt acagtcgcta ttaattaacc
    10501 tcggcttagg ttaccccttc agtttgctct acaccgctgc gtttacggct attttgcttt
    10561 tgctatggcg aacattgcct cgcgtacaaa aagttctggt cggtatcagt tcgctggtgg
    10621 cggcttgtta tttccctttt gctcaggcct acggcgcgcc taacttcaat acattgctgg
    10681 cattgcactc caccaatatg gaagagtcga ccgaaatcct gacgattttt ccgtggtaca
    10741 gctacctggt cggcatattt atttttgcgc tcggcgtaat agcaatcagg cgaaaaaaag
    10801 agagtgaaaa agcgcgctgg aataccttcg acagcctgtg cctggtattc agtgtggcga
    10861 cattttttgt tgctcccgtg caaaacctgg cctggggcgg cgtatttaaa ctgaaagata
    10921 ctggctatcc ggtatttcgt tttgctaagg atgtcatcgt caataataac gaggtgattg
    10981 aagagcaaga acggatggca aaactttccg gaatgaaaga tacctggacg gtcactgccg
    11041 ttaagccgaa gtatcagacc tatgtggtgg tgatcggaga aagcgcgcgt cgcgatgccc
    11101 tcggtgcctt tggcggtcac tgggacaata ccccgtttgc cagcagcgtt aacggtttga
    11161 tatttgctga ctacattgcc gccagtggct ccacgcagaa atcgcttggc ttaacgctca
    11221 atcgcgttgt cgatggcaaa ccacagtttc aggataactt tgtcaccctg gcaaatcgcg
    11281 cgggcttcca gacctggtgg ttttccaacc agggccaaat cggcgaatac gataccgcca
    11341 tcgccagcat cgccaaacga gcagatgaag tgtacttcct gaaagaaggt aattttgaag
    11401 cagataaaaa cacgaaagac gaagcgttac tggatatgac cgctcaagtg ctggcgcaag
    11461 agcactcgca acctcagctg attgttctac atctgatggg ctcgcatccg caggcctgcg
    11521 accggacaca aggaaaatac gaaacctttg tgcaatcgaa agaaacgtcg tgctatctct
    11581 ataccatgac gcaaacagac gatttactgc gcaagctgta cgatcagtta cgcaacagcg
    11641 gcagcagctt ctcgctggtt tacttttctg accacggtct ggcctttaaa gagcgcggca
    11701 aagacgtgca ataccttgcc catgatgata agtatcagca aaatttccag gtgcctttta
    11761 tggtcatttc cagcgacgat aaagcgcatc gggtgattaa agcccgccgc tcggccaatg
    11821 acttcttagg ctttttctcg cagtggacgg gaattaaagc gaaggaaata aatatcaaat
    11881 acccgtttat atctgagaag aaagccgggc cgatatacat caccaacttc cagttacaga
    11941 aggtggatta caaccatctc ggaaccgata ttttcgaccc gaaaccttaa aacaaaaaaa
    12001 tccgccccga gaggcggatt ttttatatca ccgaagtgat tagaagcggt aaccaacacc
    12061 ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag agaagtccag
    12121 agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac cgtagtcgct
    12181 ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac ccacacccac
    12241 tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag tgatgccgta
    12301 gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg tgtaagtgaa
    12361 agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca ggttgaaacc
    12421 gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag ttacagtaga
    12481 agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg ctgaaagaca
    12541 tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt tttaaatata
    12601 tcaaaaatta atgggaaact cttcgcgatt tatgatgtct aacgggccat ttcatgtaac
    12661 agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag agaactgcgt
    12721 aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac cagactaatg
    12781 attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca acaaatgctc
    12841 aaccagcatt gggtatatcc agtacactcc acgctttact taggtttaga tatttgtggg
    12901 agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaatag tcatattgct
    12961 cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt ttcctctggt
    13021 gggcgcaccg ggggtcactg cgctgcgtct ggcattaggc acgctgatcc tcatcgcgtt
    13081 ctttaagcca tggcgactgc gctttgccaa agagcaacga ttaccgctgt tgttttacgg
    13141 cgtttcgctg ggtggaatga attatctttt ttatctttct attcagacgg taccgctggg
    13201 tattgcggtg gcgctggagt tcaccggacc actggcggtg gcactgttct cttctcgtcg
    13261 cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc tgctaccgct
    13321 ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg gggccggggc
    13381 ttgttgggct atttacattt taagtgggca acgtgctgga gcggaacatg gccctgcgac
    13441 ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggaa cgcttcaggc
    13501 tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg ctattctctc
    13561 gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa cacgcacatt
    13621 tggtacgcta atgagcatgg aaccggcgct ggctgccgtt tccgggatga ttttcctcgg
    13681 agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg cttcaatggg
    13741 ttctacgctg acagtacgca aagagagcaa aataaaagaa ttagacataa attaaattta
    13801 catttcagca tggttatgca taaccatgca gattttctcg ctacttttcc tctacaccgt
    13861 ctttatatat cgaattatgc aaaagcatat ttattccgaa aattcctggc gagcaaataa
    13921 ataagaattg ttcttattaa tatatctaac tcattgagtt tttattggtt ttgttttttc
    13981 acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca catagctggt
    14041 gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga aattatgagt
    14101 accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa cgatgtctct
    14161 gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca gtttattgat
    14221 ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt cattgccgta
    14281 catgaaatgc tggatggctt ccgcactgca ctgatcgatc atctggatac catggcagaa
    14341 cgtgcagtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa cagcaaaact
    14401 ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa agagctggct
    14461 gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc gaaagatgac
    14521 gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct gtggtttatc
    14581 gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtaca cgataaaagc
    14641 ggcttcctga caggaggccg ttttgttttg tagcccgcct caacgcactt atttagtgca
    14701 tccatctgct atcaccagct ggttaagtaa attttttgta tccacatcat cacacaatcg
    14761 ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg ttgagtgcac
    14821 aattttagcg caccagtttg gtgccccaga atggtgcatc ttcagggtat tgccctataa
    14881 atcgtgcatc acgtttttgc cgcatctcga aaaatcaatg agttgcaaaa ctggcacgat
    14941 tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa aaggaaatgc
    15001 tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggcttttg cggtttcttc
    15061 tcatgccgcg gataaaaaat tagttgtcgc gacggatact gccttcgttc cgtttgaatt
    15121 taaacagggc gataaatacg tgggctttga cgttgatctg tgggctgcca tcgctaaaga
    15181 actgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc cggcactgca
    15241 aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc gtaaaaaagc
    15301 gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga aagctaacaa
    15361 taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga agagcggcac
    15421 tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc agttcccgaa
    15481 catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc tgcacgatac
    15541 gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag cggtaggtga
    15601 ctctctggaa gcacagcaat acggtattgc gttcccgaaa ggtagcgacg agctgcgtga
    15661 caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg aaatctacaa
    15721 aaaatggttc ggtactgaac cgaaataata acgcggcgcc tgtaaagcgc actggcagtt
    15781 ccctctcccc gccggggaga ggattagggt gaagggcgca aacccgctcc ggggccatta
    15841 attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc agtttgactg
    15901 gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga ccctgtggat
    15961 ttcggtcctc ggtctggctg gcggtctggt gatcggattg ttggcaggtt ttgcacgcac
    16021 cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga tccgcggcac
    16081 acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt ttaacgattt
    16141 acgcatcgac ccgtttactg cggcggtggt caccatcatg atcaactccg gcgcgtacat
    16201 tgcggaaatc acgcgtggtg cggtgctgtc tattcacaaa ggttttcgtg aagcaggact
    16261 ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc tggcactgcg
    16321 tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca cttcgctgtt
    16381 tattgttatc ggcgtggctg aactgacccg tcaggggcaa gaaattattg ccggtaactt
    16441 ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta ccctggtgct
    16501 gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga atttaaaaac
    16561 gtctccaagc attttggtcc aacccaggtg ctgcacaata tcgatttgaa cattgctcag
    16621 ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct gctgcgctgc
    16681 atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct gaaggttaac
    16741 gatccgaaag ttgatgagcg cctgattcgc caggaagcgg gtatggtgtt ccagcagttt
    16801 tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt ttggcccgct acgcgtgcgt
    16861 ggcgcgaata aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa agtcggtctg
    16921 gcagagcgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca gcgtgtggcg
    16981 attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc gacttccgct
    17041 cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc tgaagaaggg
    17101 atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc ttcgcgcctg
    17161 atctttatcg acaaaggccg gattgcagaa gatggcgatc cgcaggtgtt gatcaagaac
    17221 ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaacttct ctgcctccct
    17281 ttcccccgaa agggaggcac accagattcc tctcatttaa aatcgcccag cctccagcat
    17341 ctatacttat ctttttgctc tgttttctca ctggaggagt catgcggtgg atcctgttca
    17401 tcctcttctg cctgttgggc gcacctgccc acgcggtatc catacccggc attacaacca
    17461 caacgacaac ggactcaacg actgaaccgg ccccggaacc ggatatcgaa caaaaaaaag
    17521 cggcctatgg cgcactggcg gatgtgctgg ataatgacac ctcgcgtaaa gagttgatcg
    17581 accagttgcg caccgtcgcc gcgacgcccc ctgcggaacc cgtaccgaag atcgtgccgc
    17641 cgacgctggt tgaagagcaa accgtgctgc aaaaagtcac cgaagtcagc cgccattatg
    17701 gtgaagccct ttccgcccgc ttcggacaac tttatcgcaa tatcaccggc tccccgcata
    17761 agccgtttaa tccacaaacc ttcagcaatg cgttgaccca tttttcaatg ttagcggtat
    17821 tagtgtttgg tttttactgg ctgattcgcc tgtgcgcact tccgctgtat cgcaaaatgg
    17881 gccagtgggc gcggcaaaaa aatcgtgagc gcagtaactg gttgcagctt ccggcgatga
    17941 ttatcggggc gtttattatc gacctgctgt tactggcact gacattattt gtcggtcagg
    18001 ttttaagcga caacctgaat gcgggcagtc gcaccatcgc tttccaacaa agtttgtttc
    18061 tcaacgcctt tgccctgatt gaatttttca aagccgtact acgcctgatt ttttgcccaa
    18121 acgtggctga actgcgcccg ttcacgattc atgacgagac cgcccgttac tggagtcgtc
    18181 gcctgagctg gttaagcagc ctgattggct atggtctgat tgtggctgtg ccgattatct
    18241 ctaatcaggt gaatgtacag ataggtgcgc tggcgaacgt catcattatg ctgtgcatga
    18301 ccgtctgggc gttgtacctg atttttcgta ataaaaaaga gatcacccag catttgctca
    18361 actttgcgga gcattcgctg gcctttttca gcctgtttat ccgcgccttt gcgctggtgt
    18421 ggcactggct ggcaagcgcc tattttatcg tgctgttttt cttttcgttg ttcgatccgg
    18481 gcaacagcct gaaatttatg atgggtgcaa cggtgcgcag cctggcgatt attggtatcg
    18541 cagcatttgt ttccggtatg ttttcccgct ggttggcgaa aaccatcacc ctctcgccac
    18601 atactcagcg taactatccc gagctgcaaa aacggctgaa tggatggctt tcggcggcac
    18661 tgaaaacagc gcgtattctg acagtctgcg tggcggtaat gctgctgttg agcgcatggg
    18721 gattgttcga tttctggaac tggctgcaaa acggagcggg gcagaaaacc gtagatatcc
    18781 tgatccgtat cgcactcatt cttttcttct cggcggttgg ctggacagtg ctcgccagtt
    18841 tgatagaaaa ccggctggct tcggatattc atggccgccc gctacccagc gcccgtacgc
    18901 gaaccctgct gacgctgttc cgtaacgcgc tggcggtgat tatcagtacc atcaccatca
    18961 tgattgtgtt gtcggaaatc ggcgtcaata tcgcgccatt gctggcaggt gccggggcat
    19021 taggtctggc tatctcgttt ggttcgcaaa cgctggtgaa agatattatt accggagtat
    19081 ttattcagtt tgaaaacggc atgaacactg gagatttggt gactatcggg ccgttgaccg
    19141 gcactgtgga acggatgtcg attcgctccg tgggcgtgcg ccaggatacc ggggcgtatc
    19201 acatcattcc gtggtcttcg ataaccacct ttgctaactt cgtccgcggt attggttcgg
    19261 tagtggcaaa ctacgatgtt gatcgccatg aagatgctga taaagccaat caggcactga
    19321 aagatgcggt agcagaattg atggaaaacg aagaaattcg cgggctggtt attggtgaac
    19381 cgaactttgc cgggattgtc ggcttaagca ataccgcgtt tacactgcgt gtttcgttca
    19441 ccacgctgcc actcaaacag tggacggtcc gctttgccct cgacagccag gtgaaaaaac
    19501 atttcgacct ggcgggtgtt cgcgcgccgg tgcagactta tcaggtgctg ccagctccgg
    19561 gcgcggcccc ggctgaaccg ttgccgccgg gggagccaac gctttaacgc tggcgattga
    19621 caaaacggcg gcgctgctcg tcgttcataa aggtccaggc tataaagcgg ctctgctttt
    19681 gcccctgggc catctctttt tttaccacct tcaccgcacc tacgtccgtc agagcacgat
    19741 acaacggcgg taagttttca ccacgagata ccagcgaggt aaaccacatc acctgcttcg
    19801 cgaagccttt gctctcttca atcatctttt tgataaaagc gacctcaccg ccttcacacc
    19861 acaactcctg ttgctgacca ccaaagttca gtgcatcgtc tttgttcagc cccaggttac
    19921 ggcgtttacg ttcactacct gcccgtgccg cagcggcgga atcgtggaat ggcgggttac
    19981 acaaggtcgc atcgtattgc tcgtttttat ggatgatgcc gttaaaaatc gccccactct
    20041 ctttttgccg acgcagacga atagcgcggt taagccccgg attagcactg atgatcgcct
    20101 gcgcactgct taacgcctgg ctgctggttt cactaccggt aaaacgccag ccatattcat
    20161 gtacgccaat cagcggataa atacagttcg caccaacgcc gatatccaga atgctggcat
    20221 tagctggaat tgttccgctt gcttcagcca gtaaatcggc aaggtgatga atataatccg
    20281 cccggcccgg tactggcggg cagagaaaac cgtccgggat atcccagttc gctacggcgt
    20341 aaaaatgggc cagcaacgcc ttattgagcg ccttcaccgc cagcggattg gcaaagtcta
    20401 cgctttgctc cccggcgggt gtaagcgtga ggaattgcct gagttcagga ttgacctgac
    20461 aaagcgtggc gagatcgtag cggctgtgat gacggttgcg cggatgcaac cccggtttct
    20521 gggcggacat agcattctcc tttaagcagc ggcgtaagat acccgcataa cgcgacaggg
    20581 taaatatctg gctgcgttta tcatttctaa aaaccttcac atggctatgg tttatggcaa
    20641 ttcaccataa aaatcatcat catgcatgtc attgatttgt cttattttgt aaaaatatat
    20701 cgttcaaaaa ataagcgtat ctctgtcaat tttttgcgtc attttcatcg cgcaggcgac
    20761 ctacacttat ctggcactac ccgttgtatt tatgaggatg tcattatgaa aaagtgcctc
    20821 acactactga ttgccaccgt cctgagcgga atctctctca cggcttatgc cgcgcaaccg
    20881 atgagtaacc tggacagtgg tcaactacgg cccgccggta ccgtttcggc aactggcgca
    20941 tcaaacctaa gcgatctgga ggataaactg gcagaaaaag cgcgcgggta atgactccaa
    21001 cttattgata gtgttttatg ttcagataat gcccgatgac tttgtcatgc agctccacc
//
LOCUS       DAZGJT010000081        20602 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZGJT010000081 DAZGJT010000000
VERSION     DAZGJT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.081"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..458)
                     /locus_tag="ACHKFK_003356"
     CDS             complement(<1..458)
                     /locus_tag="ACHKFK_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO9053624.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVE"
     gene            complement(536..1528)
                     /gene="xylF"
                     /locus_tag="ACHKFK_003357"
     CDS             complement(536..1528)
                     /gene="xylF"
                     /locus_tag="ACHKFK_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9053625.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENTLKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            1894..3216
                     /gene="xylA"
                     /locus_tag="ACHKFK_003358"
     CDS             1894..3216
                     /gene="xylA"
                     /locus_tag="ACHKFK_003358"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO9053626.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            3288..4742
                     /gene="xylB"
                     /locus_tag="ACHKFK_003359"
     CDS             3288..4742
                     /gene="xylB"
                     /locus_tag="ACHKFK_003359"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO9053627.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVAAQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMQALGVQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDLMLQACHLSRDQMPALYEGSEITGALLPEVAK
                     AWGMAAVPVIAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGVLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLSNVPALITAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            4911..5252
                     /gene="yiaB"
                     /locus_tag="ACHKFK_003360"
     CDS             4911..5252
                     /gene="yiaB"
                     /locus_tag="ACHKFK_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO9053628.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDDFFIHVCQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSA"
     gene            5298..5735
                     /gene="yiaA"
                     /locus_tag="ACHKFK_003361"
     CDS             5298..5735
                     /gene="yiaA"
                     /locus_tag="ACHKFK_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO9053629.1"
                     /translation="MDNKISTYSPAFSIVSWVALIGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTPIYYMTCLTVFIISVALLLVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5776..6771)
                     /gene="wecH"
                     /locus_tag="ACHKFK_003362"
     CDS             complement(5776..6771)
                     /gene="wecH"
                     /locus_tag="ACHKFK_003362"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO9053630.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPITWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQSRHFLRIGLCLFFYSAIALLYIALFTSINV
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLYTRTICGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            6946..7245
                     /gene="ysaB"
                     /locus_tag="ACHKFK_003363"
     CDS             6946..7245
                     /gene="ysaB"
                     /locus_tag="ACHKFK_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO9053631.1"
                     /translation="MMMNAFFPAMALIVLVGCSTPPPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            7340..8251
                     /gene="glyQ"
                     /locus_tag="ACHKFK_003364"
     CDS             7340..8251
                     /gene="glyQ"
                     /locus_tag="ACHKFK_003364"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO9053632.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            8261..10330
                     /gene="glyS"
                     /locus_tag="ACHKFK_003365"
     CDS             8261..10330
                     /gene="glyS"
                     /locus_tag="ACHKFK_003365"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO9053633.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPSEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRINRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            10653..10805
                     /gene="hokA"
                     /locus_tag="ACHKFK_003366"
     CDS             10653..10805
                     /gene="hokA"
                     /locus_tag="ACHKFK_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO9053634.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACKLKE"
     gene            complement(10782..10853)
                     /locus_tag="ACHKFK_003367"
     CDS             complement(10782..10853)
                     /locus_tag="ACHKFK_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053635.1"
                     /translation="MRNTVRKAGIISRRRRLLFQFAG"
     gene            complement(10993..11205)
                     /gene="cspA"
                     /locus_tag="ACHKFK_003368"
     CDS             complement(10993..11205)
                     /gene="cspA"
                     /locus_tag="ACHKFK_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO9053636.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(11486..11776)
                     /gene="yiaG"
                     /locus_tag="ACHKFK_003369"
     CDS             complement(11486..11776)
                     /gene="yiaG"
                     /locus_tag="ACHKFK_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO9053637.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSDELKLMRLIQANPALSKQLME"
     gene            12210..12920
                     /gene="yiaF"
                     /locus_tag="ACHKFK_003370"
     CDS             12210..12920
                     /gene="yiaF"
                     /locus_tag="ACHKFK_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO9053638.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(12970..13944)
                     /gene="ghrB"
                     /locus_tag="ACHKFK_003371"
     CDS             complement(12970..13944)
                     /gene="ghrB"
                     /locus_tag="ACHKFK_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO9053639.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETYHLF
                     GAEQFGKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(14048..14707)
                     /gene="yiaD"
                     /locus_tag="ACHKFK_003372"
     CDS             complement(14048..14707)
                     /gene="yiaD"
                     /locus_tag="ACHKFK_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO9053640.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGSHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTSEGKAQNRRVEITLSP
                     L"
     gene            14860..17193
                     /gene="bisC"
                     /locus_tag="ACHKFK_003373"
     CDS             14860..17193
                     /gene="bisC"
                     /locus_tag="ACHKFK_003373"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO9053641.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALELIHHQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETIDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMLVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRAAVLSSMQGSLPGGTDAVDK
                     IPVARIVEALENPGGAYQHNGMDRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRDEARNDF
                     DVFADLSERWEKGGYTRFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFRRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLAP
                     DEWQGSAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQTRGIQD
                     GDTVRLWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTAGGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(17162..17602)
                     /gene="yiaC"
                     /locus_tag="ACHKFK_003374"
     CDS             complement(17162..17602)
                     /gene="yiaC"
                     /locus_tag="ACHKFK_003374"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9053642.1"
                     /translation="MIREVQRSELPAILQLWLESTTWGHPFIKSSYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAIDFYHAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(17599..18162)
                     /gene="tag"
                     /locus_tag="ACHKFK_003375"
     CDS             complement(17599..18162)
                     /gene="tag"
                     /locus_tag="ACHKFK_003375"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO9053643.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCHPGNKP"
     gene            18320..19018
                     /gene="yhjY"
                     /locus_tag="ACHKFK_003376"
     CDS             18320..19018
                     /gene="yhjY"
                     /locus_tag="ACHKFK_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO9053644.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHF
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLADPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALSQAENTTN
                     NSDYLYTMGVSARF"
     gene            19247..20449
                     /gene="yhjX"
                     /locus_tag="ACHKFK_003377"
     CDS             19247..20449
                     /gene="yhjX"
                     /locus_tag="ACHKFK_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053645.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLGAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTHLLE
                     TVGLEKTFVIWGAIVLVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDAISAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVIALVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFDGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         5057 a         4902 c         5258 g         5385 t
ORIGIN      
        1 atttcaacca gttgttgttg cccaagtcct aaatcgccaa cgcgggtatc aggtgaaatg
       61 gataaactga cctgtgcgag cagcttctga cagcgtagcg tcatcaggtc ataatccata
      121 atgccattat gggttatttc gttacccagg aagatatttt ccagcacggt cagttctttc
      181 accagcgcca attcctggtg aataatggcg atacctttgc gttcggtatc gcggatgtga
      241 ctcgcctgaa tctcttctcc cgcaaaaata atttcgcctt cgtaggaacc atggggataa
      301 ataccacaca gcactttcat cagcgttgat ttacctgacc cattttcccc acagagtgag
      361 acgatttcgc cagcattcaa ccgcaagctg acgttatcaa tcgccttcac actgccgaag
      421 gttttggtaa tgttcttcat ttcaagtaga taaggcataa cgactccacc taagccaatc
      481 aattcacgcg gcatggagag aaatcacgcc cccgctccgc gccggggcgt aacgcttaca
      541 gctcgctctc tttgtggaat ccgtctttaa ctaccgtatc tttgatgttg tttttattca
      601 catcgatcgg tgtcagcagg cgggagggaa catctttcag gccattattc agtgtggtat
      661 ccgcttttgg ctcctgacca ttgcccaact caacggcaat ttctgcggca gtatttgcca
      721 acaatgtaat aggtttatac accgtcatag tttgcgtacc ggcagcaata cgtttaatac
      781 ctgcaagatc cgcatcctgg ccggaaattg ctactttccc tgataaacct tgcgcgctta
      841 atgcctgaat tgcccctcct gcggtggcat cgtttgaggc aactacagca tcaattttgt
      901 tattattggc ggttagcgcg ttttccataa ttttcaatgt gttttccggt aaccagccat
      961 caacccattg gtcaccaacg actttaattt ttccggaatc aacataaggt tttaatactt
     1021 tcatttgccc ggcgcggaac agcttggcgt tgttatctac cggcgagccg cccatcagga
     1081 agtaattacc ttgcggaaca atatcgacca gggcttttgc ctgcagttca ccgacttttt
     1141 cgttatcgaa agaaatataa aaatcgatat ccgcatcgtt aatcatacgg tcgtaagcta
     1201 atactttaat accttcttgt ttggcttctt ttacaacgtt acttaatacc tgaccgttat
     1261 aaggaataat gacaagaaca tcgacacccc ggttaatcat gttttcaatc tgcgacattt
     1321 gtgtttcttc attgccattt gcagactgta caaatacttt cgcgccgaga gattctgctt
     1381 ttttcacaaa aatatctcga tctttttgcc agcgttcaag acggagatca tcaatcgcca
     1441 tacctatttt gacttctttg gcgtgtgccg caacgttggt aagcagaagt gaggtgcaaa
     1501 gggtgagtag aatgttcttt attttcatgg tgtagggcct tctgtagtta gaggacagct
     1561 ttaataagta acaatcaccg cgataaacgt aaccaatttt tagcaactaa acaggggaaa
     1621 acaattacag atttttatct ttcgattacg atttttggtt tatttcttga tttatgaccg
     1681 agatcttact tttgttgcgc aattgtactt attgcatttt tctcttcgag gaattaccca
     1741 gtttcatcat cccattttat tttgcgagcg agcgcacact tgtgaattat ctcaatagca
     1801 gtgtgaaata acataattga gcaactgaaa gggagtgccc aatattacga catcatccat
     1861 cacccgcggc attacctgat tatggagttc aatatgcaag cctattttga ccagctcgat
     1921 cgcgttcgtt atgaaggctc aaaatcctca aacccgttag cattccgtca ctacaatccc
     1981 gacgaactgg tgttgggcaa gcgtatggaa gagcacttgc gttttgccgc ctgctactgg
     2041 cacaccttct gctggaacgg ggcggatatg tttggtgtgg gggcgtttaa tcgtccgtgg
     2101 cagcagcctg gtgaggcact ggcgttggcg aagcgtaaag cagatgtcgc atttgagttt
     2161 ttccacaagt tacatgtgcc attttattgc ttccacgatg tggatgtttc ccctgagggc
     2221 gcgtcgttaa aagagtacat caataatttt gcgcaaatgg ttgatgtcct ggcaggcaag
     2281 caagaagaga gcggcgtgaa gctgctgtgg ggaaccgcta actgctttac aaaccctcgt
     2341 tatggcgcgg gtgcggcgac gaacccagat cctgaagtct tcagttgggc ggcaacgcaa
     2401 gttgttacag cgatggaagc aacccataaa ttgggcggtg aaaactatgt cctgtggggc
     2461 ggtcgtgaag gttacgaaac gctgttaaat accgacttgc gtcaggagcg tgaacagttg
     2521 ggccgcttta tgcagatggt ggttgagcat aaacataaaa tcggtttcca gggcacgttg
     2581 cttatcgaac cgaaaccgca agaaccgaca aaacatcaat atgattacga tgctgcgacg
     2641 gtctatggct tcctgaaaca gtttggtctg gaaaaagaga ttaaactgaa cattgaagct
     2701 aaccacgcga cgttggcagg tcactctttc catcatgaaa tagccaccgc cattgcgctt
     2761 ggcctgttcg gttctgtcga cgccaaccgt ggcgatgcgc aactgggctg ggacaccgac
     2821 cagttcccga acagtgtgga agagaatgcg ctggtgatgt atgaaattct caaagcaggc
     2881 ggtttcacca ccggtggtct gaacttcgat gccaaagtac gtcgtcaaag tactgataaa
     2941 tatgatctgt tttacggtca tatcggcgcg atggatacga tggcgttggc gctgaaaatt
     3001 gcagcgcgca tgattgaaga tggcgagctg gataaacgca tcgcgcagcg ttattccggc
     3061 tggaatagtg aattaggcca gcaaatcctg aaaggccaaa tgtcactggc agatttagcc
     3121 aaatatgctc aggaacataa tttgtctccg gtgcatcaga gtggtcgcca ggagcaactg
     3181 gaaaatctgg taaatcatta tctgttcgac aaataacggc taactgtgca gtccgttggc
     3241 ccggttatca gtagcgatac cgggcatttt tttaaggaac gatcgatatg tatatcggga
     3301 tagatcttgg cacctcgggc gtaaaagtta ttttgctcaa cgagcagggt gaggtggttg
     3361 ctgcgcaaac ggaaaagctg accgtttcgc gcccgcatcc actctggtcg gaacaagacc
     3421 cggaacagtg gtggcaggca actgatcgcg caatgcaagc tctgggcgtt cagcattctc
     3481 tgcaagacgt taaagcatta ggtattgccg gacagatgca cggtgcaacc ttactggatg
     3541 ctcagcaacg ggtgttacgc cctgccattt tgtggaacga cgggcgctgt gcgcaagagt
     3601 gcactttgct ggaagcgcga gttccgcaat cgcgggtgat taccggcaac ctgatgatgc
     3661 ccggatttac tgcgcctaaa ttgctatggg ttcagcggca tgagccggag atattccgtc
     3721 aaatcgataa agtattatta ccgaaagatt acttgcgtct gcgtatgacg ggggagtttg
     3781 ccagcgatat gtctgacgca gctggcacta tgtggctgga tgtcgcaaag cgtgactgga
     3841 gcgatctcat gctgcaggct tgccacttat ctcgtgacca gatgcccgca ttatacgaag
     3901 gcagcgaaat tactggtgct ttgttacctg aagttgcgaa agcgtggggt atggcggcgg
     3961 tgccagttat cgcaggcggt ggcgacaatg cagctggtgc ggttggtgtg ggaatggttg
     4021 atgctaatca ggcaatgtta tcgctgggga cgtcgggggt ctattttgct gtcagcgaag
     4081 gggtcttaag caagccagaa agcgccgtac atagcttttg ccatgcgtta ccgcaacgtt
     4141 ggcatttaat gtctgtaatg ctgagtgcgg cttcgtgtct ggattgggcc gcgaaattaa
     4201 ccggcctgag caatgtccct gctttaatca ctgcagctca acaggctgat gaaagtgccg
     4261 agccagtttg gtttctgcct tatctttccg gcgagcgtac accacacaat aatccccagg
     4321 cgaaaggggt tttctttggt ttgactcatc aacatggccc caatgaactg gcgcgagcag
     4381 tgctggaagg cgtgggttat gcgctggcag atggcatgga tgtcgtgcat gcctgcggca
     4441 ttaaaccgca aagtgttacg ttgattggtg gcggggcgcg tagtgagtac tggcgtcaaa
     4501 tgctggcgga catcagcggt cagcaactgg attaccgcac gggtggcgat gtggggccag
     4561 cactgggcgc agcaaggctg gcgcagattg cggcgaatcc agagaaatcg ctcattgagt
     4621 tgttgccgca actaccgtta gaacagtcgc atctaccaga tgcgcagcgt tatgccgctt
     4681 atcagccacg acgagaaacg ttccgtcgcc tctatcagca acttctgcca ttaatggcgt
     4741 aaacgttatc ccctgcctga ccgggtgggg gataattcac atctatatat ctcagtaatt
     4801 aattaatatt tagtatgaat ttattctgaa aatcatttgt taatggcatt tttcagtttt
     4861 gtctttcgtt ggttactcgt aatgtatcgc tggtagatat ggagatcgtt atgaaaacct
     4921 caaagactgt ggcaaaacta ttatttgttg tcggggcgct ggtttatctg gttgggctat
     4981 ggatctcatg cccattgtta agtggaaaag gttattttct tggcgtgtta atgacggcaa
     5041 cttttggcaa ctatgcatat cttcgcgcag aaaaacttgg gcaactggat gattttttta
     5101 tccacgtctg tcagttggta gcgttaatca ctatcggtct cttgtttatc ggtgttttaa
     5161 acgcacctat caatgcttat gaaatggtga tctaccccat cgcctttttt gtctgcttgt
     5221 ttggtcaaat gcgtttgttt cgctcggcat gagcaacata aagttcttac acattcagga
     5281 atgaaaggaa tactgtgatg gacaacaaaa tatcaaccta ttctccggca tttagtattg
     5341 tttcatgggt agcccttatt ggcggtattg ttacctatct gttaggacta tggaatgcag
     5401 agatgcagtt aaatgaaaaa gggtattatt ttgccgtact ggtattaggt ttgttttccg
     5461 cggcgtctta tcaaaagacc gttcgggaca agtatgaagg catacctacc acccccattt
     5521 attatatgac ttgcctgacg gtctttatta tctctgttgc gttgttgctg gtaggtctat
     5581 ggaatgcaac gttattactc agcgaaaaag gcttttatgg gctggctttc ttcttaagct
     5641 tgtttggtgc agtagcggtg cagaagaata ttcgtgatgc cggaataaac ccaccaaaag
     5701 aaacacaggt tacccaggaa gaatacagcg aataattcac ataagcccgg ttagtccaat
     5761 gtaaccgggc ttttactaac tcactaatct gtttctgtcg attcgttgta ccagcataga
     5821 aagtaacaaa ctcgctgcca acgtcgcgca aaagatccaa ataatatcga gtattggcca
     5881 gtttttaagc tcaatccccc gggtgcgcag tgcatggata atcaatgcat ggaacccgta
     5941 tatacctaac gaatggcggg agattaagcc aagtccgcaa atggtacgcg tatacagcgt
     6001 gtttttaacc agagtcaata acgcgattgc gcagataaaa accatcggcc cacagtaaag
     6061 ataccaggta tcggcaaaat ttccgcgcca ctgtaattca tataatgtcc cgcgagagat
     6121 aataaaaacc cccgtcgcaa acagtgcggc gctcacccac gacagtgctt tatgctgtgt
     6181 gtccatcatc cctatagcgc ggcccaacat gccataaagg atgtagtaaa aagtatcgcc
     6241 attgatatat aagttaattg gcagccattc aaaaccgtcg attttctgcg gcacagtatt
     6301 tgggttagcg atgataccaa tcaccaccat tagcaccagc aacatttttc cgccgacgtt
     6361 cttcacctga atcagcggtg aaaccagata aatcactgca atcgcgaaga aaaaccataa
     6421 atggtaaaac actggctttt gcagcaggtt tttcagcgct aactccacat tgatggaggt
     6481 aaacagcgca atgtagagca gcgcgattgc gctataaaaa aacaggcata agccgatacg
     6541 caagaaatgg cgcgactggg cgctgcgttc gccaaaaaag agatagccgg aaatcatgaa
     6601 aaatagtggc acgctgacac gagaggcaga attcagaaca ttggcgatat cccaggtgat
     6661 ggggctaaca ctatgagcat tggtcacata ccaggtagtg gtgtgaatca tcaccaccat
     6721 taaacacgct atccctcgca ggttatcaat ccagtaaatt ttgggctgca tctgtgtctc
     6781 tgtatctggt taaaaaaagt ctgaccgata aatcattcag ttggcgcact ggaataatct
     6841 gagttttatc actacagctt atagaggctt aaggaaattc gtaagatatc agccactata
     6901 ccgatataaa taataagact cacctgcaaa ccagacggta atttaatgat gatgaacgct
     6961 ttctttccgg caatggcgct tattgtgcta gtgggttgtt ctacaccgcc acccgtgcag
     7021 aaagcacaac gggtaaaggt tgatcctctg cgttcgttga atatggaagc gttatgcaag
     7081 gatcaggcgg caaaacgtta taacaccggc gagcaaaaaa tcgacgtcac cgccttcgaa
     7141 cagttccagg gaagctatga aatgcgcggc tataccttcc ggaaagagca gtttgtctgt
     7201 tcttttgacg cggatggcca ttttttgcat ctttccatgc gttaagccct gctttttccc
     7261 gtttcgtact gtatatcttc catccagcgg gtatactgat cccttccttt aaatccacac
     7321 gtatccagca cgaaataata tgcaaaagtt tgataccagg accttccagg gcttgatcct
     7381 gaccttacag gattactggg ctcgccaggg ctgcaccatt gttcaaccat tggacatgga
     7441 agtcggcgcg ggaacctctc acccaatgac ctgtctgcgc gcgctggggc cagaaccgat
     7501 ggcggctgct tatgttcagc cttctcgtcg cccgaccgat ggtcgctacg gcgaaaaccc
     7561 caaccgttta cagcactact atcagttcca ggtggtcatt aagccatcgc cggacaatat
     7621 tcaggagctg tacctcggtt ctctgaaaga gctgggcatg gacccgacca tccacgacat
     7681 ccgtttcgtg gaagataact gggaaaaccc gacgctgggt gcctgggggc tgggctggga
     7741 agtgtggcta aacggcatgg aagtgacgca gttcacttac ttccagcagg ttggtggtct
     7801 ggaatgtaaa ccggttaccg gtgagatcac ctacggtctg gaacgtctgg ccatgtacat
     7861 tcagggcgta gacagcgttt acgacctggt ctggagcgac ggcccgctgg gtaaaaccac
     7921 ctacggcgac gtgttccatc agaacgaagt ggagcagtcc acttacaact tcgaatacgc
     7981 ggatgtggac ttcttgttca cctgcttcga gcagtacgag aaagaagcgc agcagttgct
     8041 ggcgctggaa aatccgctgc cgctgccagc ctacgagcgt attctgaaag ccgcccacag
     8101 cttcaacctg ctggatgcgc gtaaagccat ctccgtcacc gagcgtcagc gctacattct
     8161 gcgcattcgc accctgacca aagcagtggc agaagcatac tacgcttccc gtgaagccct
     8221 cggcttcccg atgtgcaaca aagataagta agaggcggct atgtctgaga aaacttttct
     8281 ggtggaaatc ggcactgaag agctgccacc aaaagcacta cgcagcctgg ctgagtcctt
     8341 tgctgcgaac tttactgcgg agctggataa cgctggcctc gcacacggca ccgttcaatg
     8401 gtttgctgct ccgcgtcgtc tggcgctgaa agtagctaac ctggcggagg cgcaaccgga
     8461 tcgtgaaata gaaaaacgcg gcccggcaat tgcccaggcg ttcgacgctg aaggcaaacc
     8521 gagcaaagcg gcagaaggtt gggcgcgtgg ttgcggtatt accgttgacc aggctgagcg
     8581 tctgactacc gataaaggcg aatggctgct gtatcgcgcc catgtgaagg gtgaaagcac
     8641 cgaagcactg ctgccgaata tggttgcgac ttcgctggcg aaactgccga tcccgaaact
     8701 gatgcgttgg ggcgcaagcg acgtgcactt cgtgcgtccg gttcacaccg tgaccctgct
     8761 gctgggcgac aaagttattc cggctaccat tctgggtatt cagtccgatc gcgtgattcg
     8821 cggccaccgc tttatgggcg agccggaatt cactatcgac aatgccgatc agtatccgga
     8881 aattctgcgt gagcgcggga aagtcatcgc cgattacgaa gaacgtaagg cgaagattaa
     8941 agccgatgct gaagaagcag cacgtaagat tggcggtaac gctgatttaa gcgaaagcct
     9001 gctggaagaa gtggcttcgc tggtggagtg gccggtcgtt ctgaccgcga aattcgaaga
     9061 gaaattcctc gcggtgccgt ctgaagcact ggtttacacc atgaaaggtg accagaagta
     9121 cttcccggtg tatgcgaacg acggcaaact gctgccgaac tttatcttcg ttgccaacat
     9181 cgaatcgaaa gatccgcagc agattatctc cggtaacgag aaagtcgttc gtccacgtct
     9241 ggcggatgcc gagttcttct tcaataccga ccgtaaaaaa cgtcttgaag ataacctgcc
     9301 gcgcctgcaa accgtgttgt tccagcaaca gttggggacg ctgcgcgaca aaactgaccg
     9361 cattcaggcg ctggctggct ggattgctga acagattggc gctgacgtta accacgctac
     9421 ccgtgcgggc ctgctgtcta agtgtgacct gatgaccaac atggtcttcg agttcaccga
     9481 cacccagggc gttatgggga tgcactacgc gcgtcacgat ggcgaagcgg aagacgtagc
     9541 tgtcgcgctg aacgaacagt atcagccgcg ctttgccggt gatgacctgc cgtctaaccc
     9601 ggtagcctgt gcgctggcga ttgctgacaa gatggatacc ctggcgggta tcttcggtat
     9661 cggccagcat ccgaaaggcg acaaagaccc gtttgcgctg cgtcgtgccg cacttggcgt
     9721 gctgcgtatt atcgttgaga agaacctcaa ccttgatctg caaacgctga ccgaagaagc
     9781 agtgcgtctg tatggcgata agctgactaa cgccaacgtg gttgatgatg ttatcgactt
     9841 tatgctcggt cgcttccgcg cctggtatca ggacgaaggt tacaccgttg acaccatcca
     9901 ggcggtactg gcgcgtcgtc cgactcgtcc ggctgatttc gatgcccgta tgaaagcggt
     9961 atcgcacttc cgtaccctgg aagcagctgc agcactggcg gcggcgaaca agcgtgtatc
    10021 taacatcctg gcgaaatctg atgaagtgct gagcgatcgc gtgaatgcct ctactctgaa
    10081 agagccggaa gaaattaaac tggcgatgca ggttgtggtg ctacgtgaca agctggagcc
    10141 gtactttgcg gaaggtcgtt accaggatgc gctggtcgaa ctggctgagc tgcgtgaacc
    10201 ggtcgatgct ttcttcgata aagtgatggt catggttgat gataaagaat tgcgtatcaa
    10261 ccgtctgacc atgctggaaa aactgcgcga actgttcctg cgcgttgcgg atatttcgct
    10321 gttgcagtaa taacgccgtt attaaaaagc ctgccatctg gcaggctttt tttatcgcta
    10381 aataatacag caacctttaa taatcttctg ctgaataaag attgtctcat atattaattt
    10441 catgagattt ttttaggatt atatcaagga gaagaaacga acttattaag ctagaatagc
    10501 tgcgggtgct tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc
    10561 cacctgacta taaatcaaag tgaggcccct ctatgcatag acaccataag gttagcctct
    10621 tactcgttgg aaatcaacac agggggctgg gaattccgca gaaatataga ttactttctt
    10681 taatagtgat ttgtttcacg cttttatttt tcacctggat gataagagat tcactgtgtg
    10741 aattgcatat taaacaggag agttatgagc tggcggcgtt tttagcctgc aaattgaaag
    10801 agtaagcgtc ttcggcggga aattattccc gcctttctta cggtgttgcg cattctcatt
    10861 gcacccaaat ttattcttca caaaaataac actagattct attacgcgat cgattattta
    10921 tttcctgaaa aacaaataaa aaaatccccg ccaaatggca gggatcttag attctgtgct
    10981 tttaagcaga gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc
    11041 gatggtgaag gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc
    11101 agagaagtgt acgaacacgt ctttagagcc atcgtcagga gtgatgaagc cgaagccttt
    11161 gtcagcgttg aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta
    11221 ataattaagt gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga
    11281 atgtgcacta cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct
    11341 ttaatggtct gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca
    11401 acaattattt ttcatattta tgattaatcg gttggaaaac ggtcctgtta tcaggaccgt
    11461 aaacagcaat aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg
    11521 cttgaatcaa acgcatcaat tttagttcgt cacttgaagg cttcacgcgt ctggattccc
    11581 attcctttac catggcgact gatacgccca aaacccgggc aaaatcatcg atttttaatc
    11641 ctgtcccttt tcgtaactgc tcaatttcgg tacaggatgt tgttctgtgc gtcagggtaa
    11701 ttttctgcgt ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg
    11761 gatctttata ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga
    11821 agctcattaa gaatagtcgg gaaaaacaga cacagcgggg tgtagtcagg gcatactctg
    11881 actatgattc gattcagcca gtcatttagc gtgacttata gccacctaac gatctgataa
    11941 atgccgatat atctcttacc actcccggtt tgtaaagatt tcgaaaatct aacgttctgc
    12001 gaaggcgtaa atctctggct acaaaaaagg caacggcaat atcatttttt aacaatgaaa
    12061 caacaaatta accttgtttc attttctcca tgattttacc aggaagatta cgtagaaagg
    12121 gtatcctgca agcctgtcct ggactatcct tgtcacgtca gacactcgtg tgtcgttgtg
    12181 cgcttttttt gggtgaaagg agtaagaaaa tggcgacagg aaagtcctgc tctcgctggt
    12241 ttgcgcctct tgcggcgtta ttaatggtag ttagcctgag tgggtgtttt gataaagaag
    12301 gcgatcagcg taaagcgttt atcgacttcc tgcagaatac agtgatgcgt agcggtgaac
    12361 gtctaccaac cctgactgcc gatcagaaaa aacagtttgg tccttttgtc tctgattacg
    12421 cgattctgta tggttattct cagcaggtga atcaggcgat ggattccggt ctgcgcccgg
    12481 ttgtagacag cgttaacgca attcgcgtac cgcaggatta cgttacgcaa agcggtccgc
    12541 tgcgtgaaat gaacggttcg ctgggtgtgc tggcgcaaca gctgcaaaat gcgaagttgc
    12601 aggccgatgc tgcgcactct gcgttaaaac agagtgatga cctgaaaccg gtctttgatc
    12661 aggcattcac caaagtggta acgacgccag ctgatgcatt gcaaccgttg attccggcgg
    12721 cacaaacttt cacgcaacaa ctagtgatgg ttggagacta cattgctcag caaggtactc
    12781 aggtgagctt tgtcgcaaat ggcatccagt tcccgacttc acagcaggca agtgaatata
    12841 acaaactgat tgcgccatta ccggcacaac atcaggcgtt taatcaggcc tggactacag
    12901 cagttactgc aacccaataa agagtaaaag cccgagcgat tatcgggctt tactcctcgc
    12961 agtcgcggct tagtccgcga cgtgcggatt cacacagttc ttctcaacct ttccttgtaa
    13021 cgcatcaatc aaattatcca cggcacaggc ggccatgcca taacgcgtct catgagtggc
    13081 agatccaata tgcggtactg cgacgacgtt ggccattgag agcaacggcg aatctacgga
    13141 cagtggctct tgttcgaaga catccagccc ggcggcgtga atttctcctt tctgcaatgc
    13201 agcaatcagt gcattttcat caaccaccgg gccacgtccg gcattaatga aaatggcgga
    13261 ggatttcatt ttgccgaatt gttctgcgcc aaatagatga tacgtctcat cagttaacgg
    13321 caggatcagg caaacgaaat ctgactcttg taacagtgta tccaaatcgc agtagcgggc
    13381 gttgaagcgt tcttctgctt ctttatggtg gcggcgcgcg ttatagagga tgggcatgtt
    13441 gaaaccaaag tgcgcacgtt gtgccagcgc catgccgatc cgtcccatcc cgacaatgcc
    13501 cagtgtttta tggtgaacgt cagtgccgta ccagtccggg cctatgctcg cggtccattc
    13561 gcctgctttt acccgttctg ctacctccac aacccgacga gcggtagaca acaccagcgc
    13621 catcagcgta tcggcgacgg tttctgttaa tacggttggt gtgtgcatca gcagaatttt
    13681 tcgggcggta agcgcatcga catcaaaatt gtcatagccg acggagatcg ttgatgtggc
    13741 acgcagtttc ggcatttttt ccagcaatgc ggcatcaaca ttctcgtttg aacccagcaa
    13801 gccttcagct tcggcaaaaa ttgctgcatt ttgttcgacg gtttgtgggc tgaggtttgc
    13861 cacctggtga acggtgaaat gttcttgcag gcgttgcagt aaatcatcag gtaaggcttt
    13921 gtagaggata acggacggct tcatgcttct ctccattcac tgataaggcg atcaccttag
    13981 cctgaaatat gatgaaggaa aactgaagaa tgccaggtga tttctgcatc acctgacatg
    14041 aaagggatta cagtgggctt aaggtaattt ctacacggcg gttttgcgcc ttaccttctg
    14101 aggtgctgtt gctggcgatt gggttagccg ggccaaggcc ttgagtacgg atgcggctgg
    14161 cgtccacgcc ctgggtgatc aacgcgctgg caacggaatc cgcacgctgc tgggagagac
    14221 gcatgttcag gtcgtgactc cccgtgctgt cggtataacc aatcacgtta accgccgttt
    14281 tcggatactc tttcagtacc attgccacgc cggtcagggt gttagcgccc gccggtttca
    14341 gggttgcgct gctgctgtcg aaggtcacat tgttcggcat attgaggata atgttatccc
    14401 cgctgcgggt tacgctaaca ccagtgccgc gcattttgtc acgcagcttc gcttcctgca
    14461 catccatgta ataaccaacg ccgccgccca gagctgcgcc tgctgctgcg ccaatgagcg
    14521 cgcctttacc gcgatctttc ttcgaagaag agagcgcacc aatacccgcg ccaacgagag
    14581 agcccagacc tgcgccgata gcagatttac ctgcttcgcg ttcgccggtg taagggttag
    14641 ttgtacagcc agataccgcc agagcgccac tcactacggc ggcaataaga taaacacgtt
    14701 tcttcattgt taatccttaa taaccttttt attctttgcc acgggttccg tggcgggaga
    14761 ttatgccgcg tgagcatgaa gattattcct gggaatactc ggaaatttgt aagtaatatt
    14821 taactgctca atacatctaa cctttcagga gtcttcggtt tggccaactc atcctcacga
    14881 tattccgttc tgactgccgc ccactggggg cctatgctgg ttgaaaccga cggcgaaacc
    14941 gtgtttagct cgcgtggcgc gttagccaca ggaatggaaa actccttgca gagcgcggtt
    15001 cgcgaccagg ttcacagcaa tacgcgggtg cgatttccaa tggtgcgcaa aggctttctt
    15061 gcgtcaccgg aaaatccgca aggcattcgt gggcaggatg aatttgttcg cgtgagttgg
    15121 gatgaggcgc tggagcttat tcaccatcaa cataaacgca ttcgtgaggc ttatggtccg
    15181 gcatcgattt ttgctggttc ctacggctgg cgttcaaacg gcgtgctgca taaggcctcg
    15241 acattattac aacgctatat ggcgctggca ggcggttata ccgggcatct gggggattat
    15301 tcgaccggcg cggcacaggc gatcatgccg tatgtcgtgg gtggcagcga agtttatcaa
    15361 cagcaaacca gttggccact ggtgctggaa catagcgatg tcgtggtgct gtggagtgct
    15421 aacccactca atacgctgaa aattgcgtgg aatgcatccg atgagcaggg gctttcttac
    15481 ttttccgcac tgcgtgacag cgggaaaaag ctgatctgca ttgatccaat gagatcggaa
    15541 accatcgatt tctttggcga taaaatggag tgggtggcac cacacatggg caccgatgtt
    15601 gcgttgatgc tggggatcgc ccatacgctg gtggaaaatg gttggcacga cgaagcgttt
    15661 ctggcgcgtt gcaccacagg ttatgccgtc ttcgcctctt atttgctggg cgagagtgac
    15721 ggtatagcga aaaacgccga atgggcggca gagatttgtg gtgttggcgc agcgaaaatc
    15781 cgcgagctgg cggctatttt ccaccaaaat acaaccatgc tgatggcagg ctggggaatg
    15841 cagcgccaac agtttggtga gcaaaaacac tggatgctcg tcacgctggc ggcaatgttg
    15901 gggcaaatcg gcacacccgg cggcggtttt ggtctttctt accattttgc caatggtggt
    15961 aaccccacgc gccgcgctgc ggtgctctct tccatgcagg gcagcttgcc gggtggcacc
    16021 gatgcggtag ataaaatccc tgtcgcccgc attgttgaag cactggaaaa ccctggtggc
    16081 gcatatcaac ataatggtat ggaccgacat ttcccggata ttcgttttat ctggtgggcg
    16141 ggtggtgcca actttactca tcatcaggat accaatcgcc tgatccgtgc ctggcaaaaa
    16201 ccggagctgg tggtgatctc cgaatgcttc tggacggctg cggcgaaaca cgcggatatc
    16261 gttctgcctg cgacaacctc ttttgagcgt aatgatctca ccatgaccgg tgattacagt
    16321 aatcagcatc tggtgccgat gaagcaagtg gtgccgccac gcgatgaagc gcgtaatgat
    16381 tttgatgttt ttgccgactt gagtgaacgt tgggagaagg gcggttatac acgttttacg
    16441 gaaggaaaaa gtgagctgca atggctggaa acgttttata acgttgcccg gcagcgcggg
    16501 gcaagccagc aggttgaatt gccaccattt gctgagttct ggcaagccaa ccagttaatt
    16561 gagatgccgg aaaacccgga cagtgagcgg tttattcgct tcgccgattt tcgccgcgat
    16621 ccgcaggcgc atccattaaa aaccgccagc ggtaagattg aaatcttctc gcagcgtatt
    16681 gccgattacg cttatccgga ttgcccgggg catccaatgt ggctggcgcc ggacgaatgg
    16741 cagggcagtg ccgaaccgga acagttgcag gtactttctg ctcatccggc gcatcgtctg
    16801 cacagccagc tgaattacag ttctctgcgc gaattgtacg cggtggcaaa tcgtgagcct
    16861 gtcaccattc atcctgacga tgcccagacg cgcggcatac aagatggcga tactgttcgg
    16921 ctgtggaact cacgcgggca aattcttgcc ggagcggtca ttagcgaggg aattaaacct
    16981 ggcgtgattt gtattcacga aggcgcatgg ccggatttgg atttaaccgc tggcggcatt
    17041 tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc ccagctcgcg gctggggaat
    17101 ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat acaacggtcc ggaactgaca
    17161 cttacagcgt ttgaaccacc ggccagctca taatccaggt gggtagttgg gtttcatcct
    17221 gccatgcgca atcgacaatg tgaaaaccct gcgcatggta gaaatctatc gccggttgat
    17281 ttttttgata aacctccagc atcagatggg gatggcgctg ctgcacatac tgcatcagcg
    17341 ccttaccaat accgcgcctg acggccttcg gtgcgacaaa cattgctgcc agaaatcggc
    17401 cttccataat actgacaaaa ccgagaagct tgccgtcttc ttcccagacc cagttttgcg
    17461 cgttggcaag atacgcatcg cgcaccagcg gaatgcagtc acgccagtaa ctcgatttta
    17521 taaagggatg cccccaggtt gtactttcca gccacagttg gaggatcgcg gggagttctg
    17581 aacgttgcac ttcccgaatc atggtttatt tcccggatgg caacagcagc caaccacatg
    17641 atcattcacc agcccacatg cctgcataaa ggagtaacag attgtggtac cgacaaactt
    17701 aaaaccacgt ttttttagtg ccttagatag ggcgtcggag gcgggtgtag atgtggggat
    17761 ttcgctcaac gttgtggctt gtgtcacctg tggttgatga ttgacaaacg accagacaaa
    17821 gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac gcccgcgcat taccaataat
    17881 tgcctgaatt ttcccacgat ggcggataat cccggcgtcc tgtaccagtc tttcgacatc
    17941 ctcttcctgc attgcggcaa ctttcaccgg atcgaactga tgaaagcatg cgcgatagtt
    18001 ttcgcgtttt ttgaggacgg tgatccacga taacccagcc tgctgccctt ccaggcagat
    18061 catttcgaac agttttttac tgtcagtttc aggcacgccc cattcattat catggtaggc
    18121 aatataaagc gggtcctgac tcacccagcc gcaacgttcc atactttccc tcgctatgta
    18181 cccgatcgta aaaatttcag ccgcttttct tgacgcatcg gtgaaaaagt caatatttaa
    18241 tacagggcta ctatgcccga tatccttcat acaatgacaa taatattgcc gaattcggct
    18301 cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt ggtcgctggc agctaagcct
    18361 gcttgcgagc gtggtaatca gtgccttttt tctcaacaca gcttacgcct ggcaacaaga
    18421 atatatcgtt gatacgcaac ccggacattt cacagagcgt tacacctggg atagcgatca
    18481 tcaacctgat tacaacgata ttttgtcgca acgtattcaa agtagccaaa gggcgctggg
    18541 actggaagtc aatctggcgg aagaaacccc tgtggatgtg accagcagta tgagtatggg
    18601 ctggaatttt cctttatatg agcaggttac aaccggcccg gtcgcggcat tacattacga
    18661 tggcacaact acctcgatgt ataacgagtt tggcgacagt actaccacgc tggccgatcc
    18721 gttatggcat gccagcgtga gtaccttagg ctggcgtgtt gactcccggc ttggcgatct
    18781 tcgaccctgg gcgcaaatca gctataacca gcaatttggc gagaatatct ggaaggcgca
    18841 atcaggcctg agccggatga cggcgacaaa ccagaacggc aactggctgg atgtcaccgt
    18901 aggcgctgat atgttgctca atcaaaatat tgctgcctat gctgcgctat ctcaggcaga
    18961 aaataccact aacaatagcg actatctgta tacgatgggg gttagtgcca gattttaacg
    19021 ttatagatac aattgaaacc cataaataac aactaatgtg gtgatagtgg ggactaactt
    19081 actaaataat aaatttggcg aataattgtc gagtcattca ttcctgaact aatgcatttc
    19141 attccgttct gatggcattt catgccgttt ttccccaggc ataaagcgca cttcgttatg
    19201 gttgtcagca gagatttttc ctttttatta ctgcaggaat actgccatga caccttcaaa
    19261 ttatcagcgt actcgctggc tgacactcat cggtactatc attacccagt ttgctctggg
    19321 gtcggtttat acctggagtc tgtttaacgg tgcgctttct gccaagctgg gggctcctgt
    19381 aagccaggtt gctttctctt tcggcttgtt aagtctgggg ctggcaattt cgtcttctgt
    19441 tgcgggcaaa ttgcaggaac gttttggtgt taaacgcgtc actatggctt ccggcatttt
    19501 gctgggatta ggtttcttcc tgacagcaca ttccaacaac ctgatgatgc tgtggttaag
    19561 cgccggtgtg ttggttggtc tggcggatgg tgcgggttac ctgttgacgc tctctaactg
    19621 cgtgaagtgg tttccggagc gtaagggact tatctctgct tttgctatcg gttcttatgg
    19681 tctgggcagc ctgggtttta aatttatcga cacgcacctg ctcgaaacgg tgggtctgga
    19741 aaaaaccttt gttatttggg gcgcgattgt actggtgatg attgtctttg gcgcaacgtt
    19801 aatgaaagat gcgccgaagc aggaagtgaa aaccagcaat ggtgtggtgg agaaggacta
    19861 caccctggca gagtcgatgc gtaaaccgca gtactggatg ttagcggtta tgttcctgac
    19921 tgcgtgcatg agtggtctgt atgtgattgg tgtagcgaaa gatatcgctc aaagcctggc
    19981 gcatcttgat gcaatttccg cagccaatgc tgtgacggtt atttccatcg ccaacctttc
    20041 tggtcgtctg gtgctgggca ttctgtctga caaaatcgcc cgtatccgtg ttatcaccat
    20101 tggtcaggtg attgcgctgg tgggtatggc ggccctgctg tttgcaccat tgaatgcagt
    20161 gacgttcttt gcagcgattg cctgtgtggc atttaacttt ggtggcacga ttacggtgtt
    20221 cccgtcactg gtcagtgagt tcttcggcct caataacctg gcgaaaaact acggcgtcat
    20281 ttacctcggt ttcggtatcg gcagcatttg tggttcgatt atcgcttcac tgtttgacgg
    20341 attctatgtg actttctacg tgattttcgc cctgctgatt ctgtcactgg cgctttctac
    20401 gaccattcgc cagccagagc agaaaatgtt gcgtgaagca catatttaga aatatgttgc
    20461 tgctgaaata aatacgatga gggagacttc gaagtctccc ttttttatgg ttgcatgtta
    20521 aaagaacaat ggcatccaaa aaatgaatat ttttcgttta tacattcttt atgtaaatat
    20581 aaatattttc ttaaaaaaac gg
//
LOCUS       DAZGJT010000082        20593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZGJT010000082 DAZGJT010000000
VERSION     DAZGJT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.082"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            188..403
                     /locus_tag="ACHKFK_003378"
     CDS             188..403
                     /locus_tag="ACHKFK_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9053646.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            408..743
                     /locus_tag="ACHKFK_003379"
                     /pseudo
     CDS             408..743
                     /locus_tag="ACHKFK_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
     gene            911..1396
                     /locus_tag="ACHKFK_003380"
     CDS             911..1396
                     /locus_tag="ACHKFK_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053647.1"
                     /translation="MKEHLQRPEEKRRDEQFAAFDDYCWKVMSRNLAECFSIHNDNFS
                     ELEWECNRPSFVVSGDAGKITISENGKVTPPSHQHSEELIEFAIDYLKNNKKQGLMKR
                     VGRCMGYLQVAAEIEALASGADKDAIVREALLRDFNTPPFKKVPAYWLHPGLTYLKVR
                     I"
     gene            1433..1966
                     /locus_tag="ACHKFK_003381"
     CDS             1433..1966
                     /locus_tag="ACHKFK_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9053648.1"
                     /translation="MNTKIKYGLSAAVLALISAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFSSTFYRKLNAGDRKGACAEIRRWIYDGGRDCRNRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            2240..2809
                     /locus_tag="ACHKFK_003382"
     CDS             2240..2809
                     /locus_tag="ACHKFK_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9053649.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKHEIRDKVIRYQEEC
                     DDVLYEYWTKGFAVNPRKMSAMEELNRACADMKRDKNIASLFGTGLNEWKLVKAAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2809..2955
                     /locus_tag="ACHKFK_003383"
     CDS             2809..2955
                     /locus_tag="ACHKFK_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053650.1"
                     /translation="MGRLMISDKLITLVKNLCVIVGISFSLMLVAIFFSTAWRVLTLS
                     GLVG"
     gene            2963..3457
                     /locus_tag="ACHKFK_003384"
     CDS             2963..3457
                     /locus_tag="ACHKFK_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9053651.1"
                     /translation="MSRVLCVVIIVLAGGCGALWLATDHYRDNAITYKAQRDKKASEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRVNATCPGT
                     VREATGTSGVDNATGPRLADTAERDYFILRERLMTMQKQLEGAQEYIRTQCTKLAFYY
                     PEDT"
     gene            3454..3678
                     /locus_tag="ACHKFK_003385"
     CDS             3454..3678
                     /locus_tag="ACHKFK_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053652.1"
                     /translation="MKKLRVTVEPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCHMVSAALQPVC"
     gene            4023..4349
                     /locus_tag="ACHKFK_003386"
     CDS             4023..4349
                     /locus_tag="ACHKFK_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO9053653.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4481..4681)
                     /locus_tag="ACHKFK_003387"
     CDS             complement(4481..4681)
                     /locus_tag="ACHKFK_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO9053654.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
     gene            4723..5088
                     /locus_tag="ACHKFK_003388"
     CDS             4723..5088
                     /locus_tag="ACHKFK_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9053655.1"
                     /translation="MAQLRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCICGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            5380..5943
                     /locus_tag="ACHKFK_003389"
     CDS             5380..5943
                     /locus_tag="ACHKFK_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9053656.1"
                     /translation="MLTTQKRKFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDP
                     EVLAFIARKQGETVEVDEVPVYRQKKTQTEECHQEAPPAEKNTPVSLPPDDRQSLTPL
                     PGIDYMEDGLPDPVKAMGRILVENLSIDPKLALDAAWRLAQFTHHKKGDAGKKSAKGD
                     AAKKAANRFAVPPPPRLVVNNDNEGNG"
     gene            5940..7601
                     /locus_tag="ACHKFK_003390"
     CDS             5940..7601
                     /locus_tag="ACHKFK_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO9053657.1"
                     /translation="MIPGWSTACPDWAVRLKKGLSIIPAPIYPEQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGEHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEQ
                     QGRRISLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVA
                     RRKSEAARFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            7665..9602
                     /locus_tag="ACHKFK_003391"
     CDS             7665..9602
                     /locus_tag="ACHKFK_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO9053658.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFMRWELMEVSAVTVPANAECTIRTIKSYDRPFS
                     AASGNRKPVVKIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMT
                     KAAEEGRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAA
                     VASAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAV
                     GAGTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQV
                     SGGAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALA
                     EAVVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQP
                     TGAIWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAP
                     DIYLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWR
                     RRRTAAVAVITGVNYGTASGG"
     gene            9647..9868
                     /locus_tag="ACHKFK_003392"
     CDS             9647..9868
                     /locus_tag="ACHKFK_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053659.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            9814..12075
                     /locus_tag="ACHKFK_003393"
     CDS             9814..12075
                     /locus_tag="ACHKFK_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9053660.1"
                     /translation="MWNLLRRTRKNQKSGRDVREAGWTSLFQAVAEPFSGAWQQGVKA
                     DPESVLSFHAVFSCISLISQDIAKMRLRLMQTDTQGIRREKRQGDIARLCRRPNAQQN
                     RIQFFELWLNAKLRHGNTVVLKIRNSRGQIKELRILDWSRVEPLVADDGEVFYRITPD
                     RNCGITEAVTVPAREVIHDRFNCFFHPLVGLPPVYAAGLAATQGHHIQANSTSFFRNG
                     GRPSGVIEIPGSITEENAKKLKSNWDSGYTGENAGKTAILSNGAKYNPTTFSPVDAQT
                     VEQLKMTAEIVCSVFRVPAYKIGVGQPPSSDNVEALEQQYYSQCLQTLIESIELLLDE
                     ALETGENESTEFDVTTLLRMDSERRMKTLGESVKNTLLTPNEARKRENLPPLAGGDAL
                     YLQQQNYSLEALSRRDAREDPFASSGKTSSGTRQEGASDGNKAISETEHDAVKAMFRG
                     DTEKMTERELSIIRALGEEFAAVLADLQRTFEGKIASQAQMFEEKLASLSVVLQKCVT
                     GDDVRPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVANIPQPADGKSLTPDDV
                     RPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVNDAVSALPAPQDGRDATALEILPAI
                     DDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVSMTGERLFSVVVRQS
                     SGQRTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSMTEDKPGEAHSSAWT
                     LAAKRGRDAGGGK"
     gene            12072..12398
                     /locus_tag="ACHKFK_003394"
     CDS             12072..12398
                     /locus_tag="ACHKFK_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9053661.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            12408..12758
                     /locus_tag="ACHKFK_003395"
     CDS             12408..12758
                     /locus_tag="ACHKFK_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9053662.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGLSGREITAASRLHVLSGPWRDCILNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            12755..13201
                     /locus_tag="ACHKFK_003396"
     CDS             12755..13201
                     /locus_tag="ACHKFK_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO9053663.1"
                     /translation="MIETLLDFSGLEEISRDLQLLSGAENNRVLREATRAGANELKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            13198..13542
                     /locus_tag="ACHKFK_003397"
     CDS             13198..13542
                     /locus_tag="ACHKFK_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO9053664.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            13609..14325
                     /locus_tag="ACHKFK_003398"
     CDS             13609..14325
                     /locus_tag="ACHKFK_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO9053665.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDKTLTVN
                     TGALLTMSVSANGGTPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCVVTDSA
                     EKAQSVTSVECTVTVSAAAG"
     gene            14340..14714
                     /locus_tag="ACHKFK_003399"
     CDS             14340..14714
                     /locus_tag="ACHKFK_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO9053666.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            14810..15019
                     /locus_tag="ACHKFK_003400"
     CDS             14810..15019
                     /locus_tag="ACHKFK_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO9053667.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            15067..18309
                     /locus_tag="ACHKFK_003401"
     CDS             15067..18309
                     /locus_tag="ACHKFK_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9053668.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AVAREQRRALAELNSQLTEIRASAAGTAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYTADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGVLRDEYAAMPETVSDGITKVENAFMAWVGGANEASG
                     VTKTLSGVLNGVAGNIDNVATAAGALVAVGVARYFGNMASGAVSATAGLVTAARNEVA
                     LAEAQLRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAART
                     AAQNALNSTTAVGSRLMTGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALAEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLSQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERARLGYAADDLGFVGDSYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGANGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            18302..18643
                     /locus_tag="ACHKFK_003402"
     CDS             18302..18643
                     /locus_tag="ACHKFK_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9053669.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLDEFLSWHGGYRAFLWKPPKHNRTVRVVCREWSITDNARYS
                     DFSCMMEQVIR"
     gene            18850..20013
                     /gene="ompC"
                     /locus_tag="ACHKFK_003403"
     CDS             18850..20013
                     /gene="ompC"
                     /locus_tag="ACHKFK_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9053670.1"
                     /translation="MKKMTVALSAVAAAVIFAAGAQAAEVYNKDGNKLDVYGRATALH
                     YFSSDKGDDGDKTYARLGFKGETQINDQLTGFGQWEYQFSGNKAESEGAAGNKTRLAF
                     AGLKFGEFGTIDYGRNYGIAYDVGSYTDVLPEFGGDGWTQTDNFMTARTSGVLTYRNT
                     DFFGLVDGLNFAAQYQGKNDRGDLRKANGDGHGFSASYEYEGFGIVAAYINADRTNNQ
                     EKGLNGTLTIEQTDPLTGKVTEKQIVVDSGSVAKGKHAEMWGVGLKYDADNLYLATTY
                     SETQNLTTFGDKGVADKAQNFEAVLQYQFDFGLRPSLAYLQSRGQDVMVGGVNHGDQD
                     LVKYIDVGATYYFNKNMSTYVDYKINLIDESEFTRKAGVATDNIVAVGMTYQF"
     gene            20223..>20593
                     /locus_tag="ACHKFK_003404"
     CDS             20223..>20593
                     /locus_tag="ACHKFK_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9053671.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMSGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNP"
BASE COUNT         4865 a         4749 c         6410 g         4569 t
ORIGIN      
        1 gcttccggaa ccacaggcgg aagatgtcac cacggaaaat ggtgatattc agccggagcc
       61 accggtgacg gatatgacag acacacagcc agaatcgggc acaatgctgt aacggtgagt
      121 cagggtcatc agtaaagagc tggtggccct tttgttgttg tgagcttccg agtacgggag
      181 acggggtatg taccagatgg aaaaaatcac aacaggtgtg tcatacacca cgtcagcggt
      241 gggaacgggc tactggttcc tgcagttgct ggacagggtt tccccgtctc agtgggcggc
      301 aataggcgtg ctggggagtc tgttgtttgg gctgctgaca tatctgacta acctgtattt
      361 caaaatcaga gaggaccgtc gtaaggcggc gcggggagag taaagcgatg aagaaaaaat
      421 acgaactggt tgttaaaggg ataaataatt acccgaataa gattactgtt actgtggcac
      481 tggaaattgg tgggtatccg tcactgttgt tgccagatgt ggcgattagt cttgaccgta
      541 ctgaagatgc cacgctggag ttttacgaag ctgaggcgaa aaagcaggcg aagcagtttt
      601 tcatggatgt tgctgccggg ttatgtgaag ggaacgaacc gttgccggaa aagcgccccg
      661 taattttaga ggcgcaggat gtgttgataa cctacaaagg aaagctaccg ggaagaatta
      721 ctgatgctct gaagatgccg ccgtcaacac tgcggtcaga aaaagatgat gttgaatcac
      781 gtattgaaaa actggagcgc tatatcgctg aattgaaaaa aagcgcccca acaaaaaatg
      841 aggtgcttgc agcagatgaa atgaaagaag ctattcttga tcgcgcgggc atctaagctg
      901 cgcttcacgg ttgaaagagc atcttcagcg gcctgaagaa aaacgccgcg atgagcagtt
      961 tgctgcgttt gacgattatt gttggaaagt gatgagcaga aatctcgcag agtgtttcag
     1021 tattcataat gataatttca gtgagctgga atgggagtgt aaccggccat cctttgttgt
     1081 atccggtgat gccgggaaaa taaccatctc agaaaatggg aaagtaacac ctccatcgca
     1141 ccagcacagt gaggagctca ttgaatttgc cattgattac ctgaagaaca ataaaaagca
     1201 ggggctgatg aagcgcgttg gccgttgcat gggatatctt caggtagccg ctgagattga
     1261 agcgctggcc agtggtgctg ataaggatgc aattgtgcgg gaggctcttc ttcgtgattt
     1321 taatactcca ccctttaaaa aagtgccggc ttactggctt catccggggc tgacttatct
     1381 taaagtgcgt atttagtggg ccagggacag cggctgaata tttaatatat ccatgaacac
     1441 caaaatcaaa tacggcctgt cggctgccgt tctggcgctg atttccgctg gtgcgcctgc
     1501 gcctgacatt ctcgaccagt ttctggatga aaaggaaggc aaccacacca cggcataccg
     1561 tgatggtgca ggtatctgga ccatctgccg tggtgccatc atggtggatg gcaaacctgt
     1621 cgttccgggc atgaagttgt cgaaggaaaa atgcgaccag gttaacgcca ttgagcgtga
     1681 taaagcgctg gcgtgggtgg agaaaaacat cagagtgccg ctgaccgaac cccagaaagc
     1741 ggggatcgcg tcattctgtc cgtacaacat tggtcccggt aagtgtttca gctcaacgtt
     1801 ttaccggaaa ctgaacgccg gagaccggaa aggtgcatgt gctgaaattc gtcgctggat
     1861 atatgacggc ggcagggact gtcggaaccg ctcaaataac tgttacgggc aggtatcgcg
     1921 gcgtgaccag gagagcgcgc tggcgtgctg gggtatcgac aggtagcaga atattttgct
     1981 gaaaaatgac gcttgctcac gcgggcggat aacacgaaat cctgcgaact ggcaaaatgc
     2041 aagtgaataa agtcaggaag attgtttcac gcagaggcac cgtaatggtg tctttgtcat
     2101 ttctgcgctt cgcacaagcg taaataaacc aaagaacctt tcaggatgag ccctggtgga
     2161 taaccggcag tggtctggtt aaccctcttt gggctggtta ttcctgtgcg cagggttcat
     2221 cactaaaagg aaataaccga tgaatatgat gaccgtgccg tttcacggcg attctcttta
     2281 tgtggttaac cataacggcg aaccatacgt tcccatgaaa cctgtcgttg cggggatggg
     2341 gctggcctgg caatcacagt tggctaagtt aagacagcgt tttgcgtcaa ctataacgga
     2401 aatcgttatg gttgctgagg atgggaaacg acgcaatatg gtgtccctgc cacttcgaaa
     2461 acttgccggc tggctacaaa ccattaatcc caacaaagta aaacacgaaa tccgcgataa
     2521 ggtcatccgg tatcaggaag agtgcgacga tgttctttac gagtactgga cgaagggttt
     2581 tgccgttaat ccccgtaaaa tgagcgcgat ggaagaactc aaccgggcat gcgctgacat
     2641 gaaacgggat aaaaacattg ccagtctgtt tggtacaggg ttaaacgaat ggaaactggt
     2701 taaagccgcg catgtatcaa aaattcgcac gttgataaac gaagcgaatc tgctgattga
     2761 ttttgtcctg gctgatacag gcaaagggaa aataacaaag gcggattgat ggggcggcta
     2821 atgatatcag ataaactcat aacactggtg aagaacctct gtgtaattgt cggcatttca
     2881 ttttcactca tgctggttgc cattttcttt tccaccgcct ggcgagtcct gacgttatcg
     2941 ggactggtgg ggtgaaagag agatgagccg tgttctgtgt gtggtgatta ttgtcctggc
     3001 gggtggctgt ggtgcgctgt ggctggcaac agaccattac cgtgataacg ccatcaccta
     3061 caaagcgcag cgcgataaaa aagccagtga gctggagctg gcaaacgcaa ccattactga
     3121 catgcagcag cgccagcgtg atgttgctgc gcttgatgcc agatactcaa gggaattagc
     3181 cgatgcgaga gctgaaaatg aaactctgcg cgctgacgtt gccgctggtc gtaagcgcct
     3241 gcgggtcaac gccacctgcc ccggtaccgt gcgtgaagcc accggcacct ccggcgtgga
     3301 taatgcaacc ggcccccgac tggcagacac cgctgaacgg gattatttca tcctcagaga
     3361 acggttgatg acaatgcaga agcagctgga aggggcgcag gaatatatcc gcactcagtg
     3421 cactaagctg gctttttatt atccggagga tacatgaaga aattacgggt aaccgtagaa
     3481 ccttttcagg gaacaattcc gttccgtatt ttgcagcgtg gtcgtgttct tgttgaaggt
     3541 tcgttcagtg gtaaatgtac gcaattacac tcccggacct ttcaggtgaa tgccacgaat
     3601 gaagagctaa ccgtggagtg tacgatgaat gccgctaaat gccacatggt atccgctgca
     3661 ttacagccag tgtgttgagc gaccttatta tccatgcgcg gtattgtcgc cgtattcctg
     3721 cattaacaga gaccgcagcc cgaccgggag actcctctgc gcgagtgtgc ggggataatc
     3781 aaaaacgata cacaccgggg tttaccgcgt taacggagcg cggcgttgtc ccctcatggt
     3841 cgctggtccg gtgcgatggt ggaagaaacc ggacgatgtg ttacctcgca agctctgtta
     3901 tgtcatgtgt ctgatttgtg atttaagtcg gataattgtc gttgccatta aacagaggat
     3961 tgatgaccga cagggtggca ttgttagaat aagacttatt cttatctgtg ccgggaatga
     4021 aaatgaaaag aaatcttccg ttaattattt tgttgtcttc tctggttatg ggctgtacgc
     4081 aacataaaac agatatgccc cgacagttgg ttaaggcatt accacaatat ccggcctatg
     4141 cagcggcaaa ttatataaag ggacgggttg atgtgaagtt tgatattggt gctgatggta
     4201 ctgtcacccg aattgagttt atccgttcag agccgcacca tttgtttgat gagcaggttg
     4261 taaaggcgat ggcaaaatgg cgatttgaga aggacaggcc gtgtaaaggc gtgaagaaaa
     4321 cgtttatctt tagtccttct gcaccctgat tatttcatca gaaattaatt atcactctgt
     4381 tgttattctg tacatcccgg cagggtaagt cttgttccgt cggatatgaa gatgaaatat
     4441 tgttggagga cagtgggtac ctgctcctgt aaccgaacgt tcatttctcg ttatttttca
     4501 tgctggccgg gcgcagatgc gttgcatctg ttgccagcct tctcctgcag gcttcaataa
     4561 cccacgctga aaagttaccg gatcctttat gctcaagggc gatgttgatc tgttcaatca
     4621 tgtgattggg gaaacggata ttgcgggttg tggttctgcg ggtccggttt ttcgatgaca
     4681 tatttatttc ctttactgat tgccatatga cggggatttt acatggctca gcttcgtaca
     4741 ctccagagca gaatcaaaac actgaatacc cgacgggtga atattctgaa gggggagcag
     4801 cgtcgtgtca gtggcagtgc acgtgtttcc ctcaagcgtc atatctggct gagggacgcc
     4861 gggcagtgct gtatctgtgg tcgtgtggtt gacctctgtg acagtgaact cgatcaccga
     4921 attgcacttc agttcggtgg tggtaatgag gagacgaatc tctggacgct ctgtaccgaa
     4981 tgccatcgac aaaagtctgc tcgtgaagcg gcgggtggta tgccggaccc gacgctgccg
     5041 gaggtgtccg gaggtagtgg cagagcggac gacatcatcg gactgtaacc cgacccgggg
     5101 ggggtatcat ccggcataaa aaaacgatcg ctttggacac cgcgccccgt ctcacgcaga
     5161 gaaaaaattc ccgtttcagg ccagttaaca tgttaactgg ctgtccgggc attttttcgg
     5221 tttttatctt tattattcag tttgttgcat ggaaaaaatg ttaactggct ttttcagcaa
     5281 atgttaacca ggcagcagtt aacatttgcg gcatgaggcg ccgggaaaaa tgggctgaac
     5341 catacccggc tgagtgcgtt ctggacccgg gaggaggctg tgctgacaac gcaaaaacga
     5401 aaatttgcgc tggcgctcat gtccgggaaa aacaaaacag cgtcagccat tgccgccggt
     5461 tattcggcga agaccgccag ggttaaaggc tcgcagctgg caaaagatcc ggaggtgctc
     5521 gcgtttattg cccgtaagca gggcgagacg gtggaggtgg atgaggttcc tgtttaccgg
     5581 cagaaaaaga cgcagacgga ggagtgccat caggaagccc ctccggcgga aaagaacacc
     5641 ccggtttcac tgccgcctga tgaccggcaa agtctcacac cactaccagg tattgattac
     5701 atggaagacg gtcttcctga tccggtaaaa gcgatgggga ggatcctggt ggaaaacctg
     5761 agcattgacc cgaaactggc actggatgcg gcctggcgac tggcacagtt cacgcaccat
     5821 aaaaaagggg atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac
     5881 cgttttgcgg tgccaccacc accccgcctg gtggtgaata acgataatga gggcaacgga
     5941 tgataccagg gtggagcacg gcctgcccgg actgggcagt acgcctgaaa aaggggctgt
     6001 cgattattcc ggctccgatt tacccggagc aggccgcaca tgccctggcg atttttaaac
     6061 aactgcggat tgtggatgca ccgggcagcc cgacgttcgg ggagtcctgt gcacagtggg
     6121 tgtttgacct ggtggcggcc ctgtttggct cctacgatgc gcagaccggt gtgcgccata
     6181 tcaaggaagt gttcattctg atacctaaaa aaaacagcaa gtccacgctg gccgccggga
     6241 tcatgatgac ggcgctgtta ctgaactggc ggcaggcggc gggctacacc attctggccc
     6301 cgaccgtgga ggtggcagcc aacgccttca accctgcccg ggatatggta cgacgcgacg
     6361 atgatctgga tgacctctgc caggtacaga cgcatatccg gaccatcacc caccgggtga
     6421 cggacaccac cctgaaggtg gtggcagccg atccgaatac ggtgtccggt atcaagtccg
     6481 tgggtacgct gattgatgag ttatggctgt ttggcaaaca gtacaaggcg gaggacatgt
     6541 tacgtgaagc cataggcggc cttgcctccc gcccggaagg gtttgtggtg tatacgacca
     6601 cccagtcgaa tgagccgcct gccggggtgt tcaggcagaa actgcagtac gcccgggatg
     6661 tgcgtgacgg caaaattcat gatccgcact ttctgccggt gatttttgag catccgccgg
     6721 agatggtgga gagcggtgag cacctgctga tggaaaacct cgccatggtc aatccgaatc
     6781 tcggttattc ggtggatgag gcgtttctgt accgggagta ccgtaaagcc cgagaagccg
     6841 gtgaagagac ttttcgcggt ttcatgtcaa aacacgccaa tgtggaaatt ggtcttgccc
     6901 tgcgttctga ccgctgggcg ggggcggatt tctgggagca gcagggcagg cgcatcagtc
     6961 tggacgatat tctgcagcgt gctgatgtgg tgacggtggg gattgacggc ggagggctgg
     7021 atgatctgct ggggatgtat gtgattgggc gtgaccggga gacccgcgaa tggctgggct
     7081 ggggccatgc ctgggcacat gaaaccgcgg tggccagacg aaaaagtgag gcagcccgtt
     7141 ttcaggattt tgttgcctgt ggggatatga ccattgtccg tcgggtcggg gatgacacgg
     7201 cggaagtggc ggagtatgtg cgtcgcattc atgaggctga gttactggac catatcggta
     7261 ttgacccgtc aggggtgggg cagattctgg attcactggc ggaagccggg atccccgacg
     7321 gaattgtggt ggggataagc cagggctgga aactgggcgg ggccattaaa accaccgagc
     7381 gcaaactggc tgaaggggtg ctggtgcatg gtgaccagcc cctgatggcc tggtgtgtcg
     7441 gcaatgcccg ggtggagcct aaaggtaacg ccattcttat caccaaacag gccagtggac
     7501 ggggaaaaat tgacccgctg atggcgctgt tcaatgcggt ctccctgatg tcccttaacc
     7561 cggaaccgaa aaagaaagaa tatgcggttt ttttcatata accctgttca cactgtaacc
     7621 atcacgaacc gctccggcgg tttttttatt ttcaggaggc tgatgtgact cttaaacggg
     7681 cctgttccct gctgacggtg aaatccttca gtgaggatga acgggtgatc accgggattg
     7741 cgtcaacgcc ttctccggat cgggatggtg acatcctgga gccggagggc gcggagtttg
     7801 gcagtgcgat cccgtttctc tggcagcatg accattcccg cccggtgggg cagtgtacgg
     7861 tgcgccgggt cagcgaaggg ctggaaatca cggcaacact ggtgaagccc gtaccggata
     7921 tgccgtcgca actggctgcc cggctggatg aggtctgggc ggccattaag accgggctgg
     7981 tcagggggct gtccgtgggc ttccgtcccc atgaatacac ctttctggac ggaggcggac
     8041 tgcattttat gcgctgggaa ctgatggagg tgtctgccgt caccgtgccc gcgaatgcgg
     8101 aatgcaccat ccggaccatt aaatcttacg accgcccgtt ttctgccgcg tccggcaacc
     8161 ggaaaccggt ggtgaaaatc gcatcttctg ccggcgctgc ggcacagtca acaaccgttt
     8221 ttcataagga aaagaccata atgaatattg gcgaacagat taaaagtttt gaaaacaagc
     8281 gtgcagcgct ggcagcctcc cttgaggagg tcatgaccaa agccgcagag gaagggcgca
     8341 cgctggatgt ggaggaggaa gagcattacg acaacaccgc agcggaaatc cgtcaggtgg
     8401 atgcgcacct gaagcgcctg cgtgaactgg aagccggtaa ggccgccacg gcgcagccgg
     8461 tgaaacaggc cggtaacggg aatgtggccg cggtggcttc tgcgccggtg atccgtgtgg
     8521 agcagaaact ggataagggg attggcttcg cccgctttgc caaatcgctg gctgcggcta
     8581 aaggcgtccg atctgaagcc ctggaagtgg cccgtcgtca gtatccggat gacagtcgtc
     8641 tgcatcatgt cctgaaatcg gcagtgggcg cggggaccac cacggatccg cagtgggcag
     8701 gcagcctgtc tgaatatcag gaatacgcac aggactttat tgattacctg cgtccgcaga
     8761 ccattatcgg gcgatttggt cagggcggga tccctgcact tcgtcaggtg ccgttcaata
     8821 tccgtgtgca cgcccaggtg tccggcggtg ctgccggctg ggtgggtgag ggtaaggcaa
     8881 aacccctgac gaagtttgat tttgaatcca tcaccttcag tcatgcgaaa gtgtcggcca
     8941 ttgcggtact gacggaagaa ttgatccgtt tttccagtcc ggctgctgat gcactggtcc
     9001 gtaatgcgct ggcggaagcg gtggtggcgc gtctggatac agactttgtg gacccgaaaa
     9061 aagccgcagt ggcagatgtc tccccggcgt ccatcaccca tgatgtgaag ggcacggcat
     9121 caaccggtaa cccggatgcg gatgcagagg ctgcgtttgg acagtttgtg gcagcaaacc
     9181 tgcagcccac cggtgcgatc tggctgatgt ccagcaccaa tgccctggca ctgtccatgc
     9241 gtaaaaatgc gctgggtcag aaggaatacc cggacatgac cctgctgggt ggctccttcc
     9301 aggggctgcc ggtgattgtc tcccagtacg tgggtgacca gctggtgctg gtgaatgccc
     9361 cggatattta tctggcggat gacggcggcg tggcagtgga tatgtcccgc gaggcatcac
     9421 tggaaatgca gtctgagccg accggcgaca gtaccacgcc gtccccggtg gagctggttt
     9481 ccatgttcca gacaggcagc gtggccatcc gtgcggagcg ctggatcaac tggcgtcgtc
     9541 gccgtaccgc ggcagtggcg gtgatcaccg gtgtgaacta cggaactgcg tccggcggct
     9601 gagtctgatg aggagggcgg gaggcgcgag cttcccgcag taactgatgg caaaaatcca
     9661 gtatctgcag ggcacgcatg atgcccgggc cggggatatc cgtgatgtgg cacagccgtg
     9721 tgcggaggtg ctggttcgcc tggggaaggc ggagtacatc acggtgcgac gtccggcagg
     9781 tcagaaaaag aaacgtgatg cggagcatgg cgaatgtgga accttttacg gcgaacccga
     9841 aaaaaccaga aatcaggacg tgacgtaaga gaggcgggct ggaccagcct gtttcaggcg
     9901 gtggctgagc ccttttccgg cgcctggcag cagggtgtga aagccgatcc ggaaagtgtc
     9961 ctctcctttc atgcggtgtt ttcttgcatt tcgctgatat cccaggatat cgccaaaatg
    10021 cggctgcgcc tgatgcagac ggatacacag gggatccgcc gtgaaaaacg gcagggggat
    10081 attgcccgtc tctgtcgtcg tcccaatgca cagcagaatc gtatccagtt ttttgaactg
    10141 tggctgaacg ccaaactgcg tcacggcaat acggtggtgc tgaaaatccg taactcccgg
    10201 gggcagatca aagaactgcg tattctggac tggagccggg ttgaacctct ggtggcggat
    10261 gacggcgagg tgttctatcg cattacaccg gaccggaact gcgggatcac ggaggcggtg
    10321 acggtgcctg ccagggaagt gatccacgac cggtttaact gtttttttca tccgcttgtg
    10381 gggctgccgc cggtgtatgc tgccgggctg gcggccacgc aggggcatca tattcaggca
    10441 aattcaacgt cttttttcag aaatggcggc aggccgtccg gggtgattga gatccccggc
    10501 agtataacgg aagaaaatgc gaaaaaactg aagagcaact gggacagcgg gtataccggc
    10561 gaaaatgcgg ggaaaacggc gatcctgagc aacggggcga aatataaccc cacgacgttt
    10621 tcaccggtgg atgcgcagac ggtggaacag ctgaaaatga cggctgaaat tgtctgttcg
    10681 gtgttccgtg tcccggccta caagattggc gttggccagc cgccttccag tgacaacgtg
    10741 gaggcgctgg agcagcagta ttattcccag tgtctgcaga cgctgattga gtccattgag
    10801 ctgttactgg atgaggcgct ggaaacaggg gaaaacgaga gcacggagtt tgacgtcacc
    10861 acgctgctga gaatggacag cgaacgtcgc atgaaaacgc tgggtgaatc ggtgaaaaat
    10921 acgcttctca cgcccaatga ggcccgtaaa cgggagaatc tgccgcccct ggcaggaggc
    10981 gatgcactgt atcttcagca gcagaactac agtctggagg cgctgtcccg tcgtgatgcc
    11041 cgtgaggatc cgttcgcgtc ttccgggaaa acgtcgtcag gcacacgcca ggagggcgcg
    11101 tctgacggta ataaggccat aagcgaaaca gagcatgatg cagtgaaagc gatgttcagg
    11161 ggggatactg agaaaatgac ggaacgggaa ctgtccatta ttcgtgcact gggagaagaa
    11221 ttcgctgcgg tgctggcgga tttacagcgc acatttgagg ggaagattgc ctcgcaggca
    11281 caaatgtttg aagaaaaact ggcttccctg tctgtggtat tacagaagtg cgtgacgggc
    11341 gatgatgtgc gtccgatgct tgaacagatg gtgaaggagg cggtaagcca tattcctgtt
    11401 ccgcgtgatg gtcgtgacta cgatccggat gttctgcaga aggcggtgaa tgatgcggtc
    11461 gcaaatattc cgcagccagc ggacggtaaa agtctcaccc cggatgatgt gcgtccgatg
    11521 cttgaacaga tggtgaagga ggcggtaagc catattcctg ttccgcgtga tggtcgtgac
    11581 tacgatccgg atgttctgca gaaggcggtg aatgatgcgg tgagtgccct gccggctccg
    11641 caggacgggc gtgatgccac ggcactggaa atactccccg ccattgacga tcaaaaatcc
    11701 tttccccggg gcacgtatgc cacacaccag ggcggactct ggcgggcgta tgaaaaaacg
    11761 cacgggatgc ggggatggga atgcctggtt gacggggtgg cggatattga cgtcagcatg
    11821 acgggtgaac ggttgttctc tgtggtggtc cggcagagca gtggccagcg tacggaaaaa
    11881 acattttccc tgccggtgat gctctaccgc ggtgtgttca gagccggtga aacctaccac
    11941 cccggcgata cggtgacgtg ggggggctcg ctgtggcact gcaacagtat gaccgaagat
    12001 aaacccggag aagctcattc atcagcctgg accctggctg caaaacgtgg gcgggatgca
    12061 ggaggcggaa aatgacggca ttactgacac tggaggagat caaggcacat ctgcgtgttg
    12121 accatgacgc ggatgatgag atgctgatgg acaaggttcg tcaggctacc gccgtgctgc
    12181 tggcctacat tcagggcagc cgggataagg tgattcgtga ggacggtgaa ctgatcccgg
    12241 gtgaggcatt aacccggatg aagggggctg ccatgcgact gaccgggatg ctgtaccgga
    12301 atccggatct tgcggagcgg gaagacctcg tgcaggggga gttgccgttt tctgtttccg
    12361 tgctgattta cgatttgcgt tgtccgacgg tgttatgagg agggggaatg gcaatatctg
    12421 caggtcgtct gacacagatg ataagtgttc tgaacccggt gttaacccgt aatgctgccg
    12481 gagaaatgac ggaagaatgg gtgtcatgcg ggaaaattca tgcggatatc cggggcagga
    12541 gcagccggga gcggatgcag tccggtgcgg aaatggcgca ggcggaaatc cgcatctggg
    12601 tgcgcggtct gtctggtcgt gaaatcacgg cggcgtcacg acttcatgtg ctgagtggtc
    12661 cctggcgtga ctgtatcctg aatgttgtcg gggtgcccgt gccggatgcg accggcgggc
    12721 gcctggaaat tctctgtcgg ctgggagggg aaaaatgatc gaaaccctgc tggatttttc
    12781 gggacttgaa gaaataagcc gtgatttgca gcttctgagt ggtgcagaaa acaaccgggt
    12841 actgcgtgag gcaacccgcg cgggggcgaa tgaactgaaa gaagaagtgg tgtcacgggc
    12901 accggtgcgc aggggaaaac tgcgccgcaa tgtggtggtc ctttcccggc gctcccgcga
    12961 tggcgggatg gaatccggtg tgcatatccg tggtgttaat ccggacaccg gcaacagtga
    13021 taacaccatg aaggcggata atccccgcaa tgccttctac tggcggtttg tggaaatggg
    13081 gaccgtgaat atgccaccgc acccgtttgt gcgcccggcg tttgatgtgc gcagtgaaca
    13141 ggcagctcag gtggcgattg cgcggatgaa ccgggccatt gatgaggtac tgagacgatg
    13201 acggaggcgg atttgtatcc tcatctggcg catcttgccg gcgggcaggt gtacccgtat
    13261 gtggtccccc tgctggatgg caggccgtcg gtggcgcttc cgtgggtggt tttcagcctg
    13321 atttcatcgg tgtcagcgga cgtgatgggc gggcaggcgg agtcctctgt gtcggtgcag
    13381 atagacgttt atgccgggac tgtgacgcag gcgcgtcaga tacgtcagga cgcccgtgaa
    13441 gccataatgc tgctggcccc ggggtccgtc agtgaaatgc aggactatat tccggaaaac
    13501 cgctgttacc gtgcaaccct ggagtttcag gtcacggtgt gactttttct ttttttctac
    13561 aaaacccata ccccgccgcg tgcgggtttt ttattatcag gaggcagaat gtctgctttg
    13621 tatgaacgct cacagctgac gcaggtgatg atttcatctg ccccggcgac tgctgaaact
    13681 atggataagg cggaatatct gcgcctggac tgcaccatca aggaagtcca gttcaccgcc
    13741 ggtcagaaac aggatattga tgtgaccacg ctctgctcca cagagcagga gaacatcaac
    13801 ggtctggggg cgtcgtccga gatttccatg tcgggtaatt tttatctgaa tcaggcccag
    13861 aacgccctgc gtgatgccta tgacaatgac gcgttgtatg cgtttaaggt gctgtttccg
    13921 tccggtaagg gctttaagtt cctggcggaa gtgcgtcagc acacctggtc atccggtacc
    13981 aacggcgtgg tggcagcaac gttttcactg cgtctgaaag gcaaaccggt gtcctttgtg
    14041 gtaccgctgg cgtttgtgaa aaatctggat aaaacactta ccgtgaatac cggtgcgctg
    14101 ctgacaatgt cagtcagtgc caacggggga acgccgccgt ataaatacgc ctggaagaag
    14161 gatggtcagc cggttgacgg gcagacgaca gacaccttca gtaagccagg tgcgcagtcc
    14221 gctgatgcgg ggaaatatac ctgcgtggtg accgattcgg cagagaaagc acagagtgtg
    14281 acgtctgttg aatgcaccgt gacagtgagc gcagccgccg gataagggga tgggtcatca
    14341 tgaaaaagga tctgaaaacg ctggcgctgg ccagactgtc agggtttcgt cataaaacgg
    14401 tgaaggtgcc ggaatggggt aatgtcagcg tggtgctgcg ggagccttcg gcagaggcct
    14461 ggtatctgtg gcaggaagtg ctcaatggtg atggagagga tgacgatacc ctgtcggtgg
    14521 tggcgaaaac ccgccgtaac ctggaagcgg atgtgacgct gttctgcgat gtcctgtgtg
    14581 atacggatct gcagcgggtg ttcactccgg acgaccgtga gcaggtgctg gccgtctatg
    14641 gtccggtaca tgcccggttg ctgcgtcagg cactggaact gatcgctgat gcagagtcgg
    14701 ccagaaaaaa gtagcccgcc cggaaattcg ctttctgatg cgacttgcgc tccgtctggg
    14761 gcgcacctta tccgaactgc ggcacagcct gagtgcgagc gaggcgatga tgtggatgga
    14821 gttcgacagg gtatccccgc tgggtgatga gcgcggggat atccgtaatg cacagatcgt
    14881 gaaagcggtt tttggggcac aggggatgaa tgttgcactg aaggacgcca tgctctgctg
    14941 gggcgaggat gaggataagc cggaggtgga tccgtttgcg gcgctggaag acgcgctgag
    15001 ctttgcagca cagtcatgaa tgatgagaac cgctgaggcg gtttttttac gcccggagaa
    15061 aggtgaatgg cgacgttacg tgaactgatt atcaaaattt cggcaaattc gcagtcattc
    15121 cagtcggaga tccagcgggc ttcccgcatg ggcagtgaat attaccggac cctgcagaat
    15181 ggcgggcgtc aggctgccgc agtcgcccgg gagcagcgac gcgccctggc tgagctgaac
    15241 agccagttga cggaaatccg cgcttcagct gccggaacgg cgggggcatt tgcaggtgcc
    15301 tttgccaccg gacacctgat ttctctggcc gatgaatgga gttccgtgaa tgcccgactg
    15361 aaacaggcgt cgcagtcatc cgatgaattt tcgtcatcac agaaagtgct gatggatatc
    15421 agccagcgga cgggcacggc attttcagat aatgcggccc tgtttgcccg ctcggcagcc
    15481 tcaatgcgtg aatatggtta cactgctgat gatgtgctga aggtgacgga ggccatttcc
    15541 acggggctga aaatctccgg tgccagtacg gcagaggcgg gctcggtgat cacccagttc
    15601 agccaggcgc tggcacaggg tgtgttacgc ggtgaggaat ttaattcggt caatgaaagc
    15661 ggagaccgga tcgtacgtgc actggctgcg ggtatgggcg tggcccgtaa agaccttaag
    15721 gcgatggcgg acgacggcaa actgacggcg gataaagtcg ttcctgcgtt aatcagccag
    15781 ctgggggtat tgcgtgatga atatgccgcc atgccggaaa cggtctctga cgggatcaca
    15841 aaggtggaaa acgcctttat ggcctgggtg ggtggcgcga atgaggccag tggggtgaca
    15901 aaaacgctct ccggcgtgct gaacggtgtt gccggtaata ttgataatgt ggcaacagca
    15961 gcgggggcgc tggttgccgt cggggttgcc cggtactttg gcaatatggc ctccggagcg
    16021 gtgtctgcca cggcaggact tgtgacggca gcacgtaatg aagtggcact ggcggaagca
    16081 cagctcaggg ggacgcagat tgccacggcg cgggcaaggg cagccgtgta ccgtgcacag
    16141 caggctgtgg cggcagcccg cgggacggag atgcagattg ctgcagaagc ccgtctggcg
    16201 gccacacagg aacgcctgaa cagaaatatt gctgccagaa ccgcagccca gaatgcgctg
    16261 aacagtacaa cggcggtggg ctcacgtctg atgactggtg cgttgggact ggttggtggc
    16321 gtacccggac tggtgatgct gggggcagca gcatggtata cgctgtacca gaatcaggag
    16381 caggccaggg agtcagcgcg ccagtatgca ctgacgatag atgaaatcgc gcataaaacg
    16441 ccgtcaatgt ctttgcctga agcctcagat aatgaaggac gaacacgggc ggcgctggca
    16501 gagcagaacc ggctgattga tgaacaggcc agccgggtga aatccctgca ggaaaaaatc
    16561 gctggatatc agtatgttct ggctaaccct ggctggacaa ccggtgacgg attcatgata
    16621 aaccatctga catcggtgaa gaccgtaacg gaagggcttt ctcaggcaac agagcagctt
    16681 gccgttgagc agtcccgtct ggcacagatg caggaaaaag cgcagtccat tcaggatgtg
    16741 cttgccgggc tggaagaccg tcgtgtggcg ttaattcgtc agcaggcggc agagcagaat
    16801 aaggtgtacc agtccatgct ggttatgaac ggtcagcata cggaattcaa ccgtctgctg
    16861 gggctgggga atgaactgct tcagcagcgg cagggactgg tgaatgtgcc gttacggctg
    16921 ccacaggcca ccctggatga taaacagcag agtgccctga caaaaacaga gcgtgagctg
    16981 gccctgtccc gactgaaggg ggaggaaaaa gagcgtgccc ggctggggta tgcggcggat
    17041 gaccttggtt ttgtggggga ttcgtatcag gaggcgagac agcgttatat cagtaatgct
    17101 ctggaagcct ggcgtaataa cgaggcgaac aaacctaaat cccggggtgg aaaatcagag
    17161 acggaaaaag cggaagacag tttttcccgg ctactgaagc agcagaaaga gcaactggca
    17221 ctggcgggtc agaatacaga gctggcgaag ctgaagtacc agactgcgca gggcgaactg
    17281 aaaaccctga cggagatgca gaagcaggaa ctgctgcgta acgcggccct gattgaccag
    17341 caaaaaatcc gggaacagtt gcgatcccgg gaagagaccc tgaagaatga taatgtggct
    17401 gcgcgtgcat caaatgaagc cgaactgctg gggtacgggc agggagaacg agccagggaa
    17461 cgcatgcggg agttgcagca gatccgcgac agcttccgcc agaaggatgc ggaccttcag
    17521 tctcagtatc agaccgggga tatcagtgag gatttttaca gacaggcacg ggcacagaac
    17581 gcgcagtatc tgagcgaacg ccttaaggac caggcagcct tttatgccga atcggatgcg
    17641 cagcgtgcgg actggcagaa agggctgcag gaggggttca gtaactgggt ggacaatgcg
    17701 tccgattacg cctcacaggc agcacagctg gcgacggagg gtatctcagg gatggtgaat
    17761 aacatcacgg agatgctgaa cgggaataaa gtggaatggc gcaactgggc ctcatccgta
    17821 ctgcaggaaa tatcaaaagt tcttatgaat gccgcgattg tcaacggaat taagacggcg
    17881 gcaaacggta tgtccggtgc gggaggattt attggcagca ttggtgactg gctgggcggt
    17941 gcggtggcca atgcaaaagg cggcgtgtat acctcggcaa acctgagcgc gtacagcaac
    18001 agtattgtgg atacgcccac gtactttgcc tttgcaaaag gggccgggct gatgggggaa
    18061 gcaggacctg aagccataat gcccctgacc cgggcggcgg atggctcgct gggtgtacgc
    18121 gcggtgggca gtatgaacgg cagtgccggt ctggtgtatt ccccggtcta ccatatcgcc
    18181 attcagaatg acggggccaa cggtcagata gggccggagg cggcaggcag ccttgttcag
    18241 ctgattgacc agcgggtgca ggcggtgatg ctgtccatgc gacgagacgg aggaatgctg
    18301 agtggctgag ataaaaacgc tgcatctggt cccgcgtgaa gggatgcagg tgagtgagaa
    18361 accgtcggtg gtgagggtcc ggtttggtga cggttatgaa cagcgccgac cgacgggact
    18421 gaatgcccga ctgaagacat ttcaggcagt gttccgggtg acggatgagg cgacccggcg
    18481 ctggctggat gaatttttat cgtggcatgg tggttaccgt gcctttttgt ggaaaccccc
    18541 gaaacataac cggacggtca gggtggtgtg ccgggagtgg agtattacgg ataacgccag
    18601 gtacagcgat ttcagttgta tgatggaaca ggtaataaga taagtttttg agaaaaaacc
    18661 taaaagcatc gtttattaat ttgcttgctt gttatttttg ttttgtcttg acataaagtg
    18721 ctatcaaagt tgttttttta cattttgaaa caaaataaaa attgaacccg taatttaatt
    18781 tacaatattt tgagttttgt tcatgacatc tctatgcatg aatagttcag tttaaataag
    18841 gattaaatca tgaaaaaaat gacagtggca ctttcagctg tggcagcagc tgtaattttt
    18901 gcagcggggg cgcaggctgc agaagtatac aataaagatg gcaataaact tgatgtgtat
    18961 gggcgtgcaa ctgcactcca ttatttctcc agtgataagg gggatgatgg agataagaca
    19021 tacgcacgtc tgggctttaa aggtgaaact cagattaatg accagttgac tggttttggt
    19081 cagtgggaat atcagttctc tggtaataaa gctgaatcag agggtgctgc gggtaataaa
    19141 acccgtcttg catttgcagg tctgaagttt ggtgagtttg gcaccattga ttatggtcgt
    19201 aactatggta ttgcgtacga tgttggttct tatactgacg tattaccaga gtttggcggt
    19261 gatggctgga cccagaccga taacttcatg acagcccgta cttccggggt attaacctat
    19321 cgtaatacag atttctttgg gcttgtcgat ggcctgaatt ttgcggcaca gtaccagggt
    19381 aaaaatgatc ggggtgatct ccggaaagct aatggtgatg gtcacggttt ctcagccagt
    19441 tatgaatatg aaggttttgg tatcgttgcc gcatatatta atgcggaccg aaccaataat
    19501 caggaaaaag gtctgaatgg tactctgacg attgaacaaa ctgatccttt aaccggtaaa
    19561 gtgactgaaa agcagatcgt tgttgattcc ggtagtgttg caaaaggtaa acacgctgag
    19621 atgtggggag tcggactaaa atacgatgct gacaaccttt acctggctac aacatattcc
    19681 gaaactcaga atctgacgac ctttggtgat aaaggagttg cagataaagc gcaaaacttt
    19741 gaagcggtac tgcagtatca gtttgatttt ggtctgcgcc cgtccctggc ctaccttcag
    19801 tcccgcggac aggatgttat ggttggtggt gtgaaccatg gcgatcagga tctggttaaa
    19861 tatattgatg tcggagcgac ttattacttt aacaagaata tgtccaccta tgttgattat
    19921 aaaattaacc tgattgatga aagtgaattt acccggaaag ccggtgttgc gacagataat
    19981 atcgttgctg tgggtatgac ttatcagttc tgattattgc tgataagtta ataaatacgg
    20041 gccgtctgcc cttacaggcg gcctgtatca atgaaaacac agttttcatt ggtcactgcg
    20101 atcagcaatt gccatctggc atgtgctgat ttaactttct gttattacct ttattggttt
    20161 tattttaaat tggactttta ttgttcgggg cgcgtctgcg cccctttttt atgggcggat
    20221 atatgcagga tattcacgaa gaaagtctga acgagtcggt taaatcagag cagtcaccgc
    20281 gggtggtact ctgggaaatc gacctgacgg tacagggcgg tgagcggtat tttttctgta
    20341 atgagctgaa tgaaaaaggg gagccggtca cctggcaggg gcgtaagtat gaggcatacc
    20401 cgattgacgg cagcggtttt gagatgagcg ggaagggcag cagtgccaga ccgtcgctga
    20461 cggtgtccaa tctgtttggt cttgtcaccg gaatggcgga ggatttgcag agcctggtgg
    20521 gggccacggt ggtccgccgc cgggtgtatg cccgttttct ggatgcggtg aatttcgttg
    20581 cgggcaatcc gga
//
LOCUS       DAZGJT010000083        20477 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZGJT010000083 DAZGJT010000000
VERSION     DAZGJT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20477
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.083"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..577
                     /gene="lpfA"
                     /locus_tag="ACHKFK_003405"
     CDS             53..577
                     /gene="lpfA"
                     /locus_tag="ACHKFK_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFO9053672.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGSVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTTTGDKSAATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     ISVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVATKNAVTI
                     GYGNAEVDFNLTYN"
     gene            661..1347
                     /gene="lpfB"
                     /locus_tag="ACHKFK_003406"
     CDS             661..1347
                     /gene="lpfB"
                     /locus_tag="ACHKFK_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO9053673.1"
                     /translation="MKRISLAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQMSINGQKIERAGMVLAKGTLNINLPAGTGNANEVKYSIINDFGV
                     AGEMLTKSIN"
     gene            1370..3901
                     /gene="lpfC"
                     /locus_tag="ACHKFK_003407"
     CDS             1370..3901
                     /gene="lpfC"
                     /locus_tag="ACHKFK_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO9053674.1"
                     /translation="MHWMHLPLDHYRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHVDLSVYDSPVGQQIPGKYRVFVFVNEEKMASRTLDFSTASEAQRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKILPPEACVAFDEIIPQATSRFDFNTQTLHLTFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGSGSSPDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKESDGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSGKPRFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKDEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSIAYTWTRSPEWDED
                     DRLWSFSLSIPLGGAWGSYRMTTDQNGKTSQQASVSGTLLEDRNLNYNVQQGYTSNGV
                     GNSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAIVRSRFDTRVGYRVLMSLKRANGNAVPFGATAALSDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGHEQAQRCRVPFRLPEKKDNSGIVMVNAVCDK"
     gene            3912..4964
                     /gene="lpfD"
                     /locus_tag="ACHKFK_003408"
     CDS             3912..4964
                     /gene="lpfD"
                     /locus_tag="ACHKFK_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO9053675.1"
                     /translation="MKIKNLLAGMVLLGTSTFTGNIWAADWGPCRTASGDPFIFVTSF
                     TKNIQNPTDNVTGQTYPDFYQWALGDKYSGVCECPSPNPTEARPTLYKTESTLAAGHN
                     STYFKITNNLEVSTRVYIANVGNVQVPFINKSNSQPGRECDQPTFGWTTGSKGQLSLY
                     IAKPFVGEQNIPQTIIVSVFGTKKENVYSSVPISQVLLSGKVTVTQGCELAAGTSLDI
                     DFGEYQAHDFKGRTGQPPQNVQKIQKELTFNCTNISDGVHIYLSLEGTPNAAYPSAIS
                     LGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEYVKRKVTTTITAYPVSTTGKLP
                     AAGDYSGVATMHVELD"
     gene            5250..5429
                     /locus_tag="ACHKFK_003409"
     CDS             5250..5429
                     /locus_tag="ACHKFK_003409"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053676.1"
                     /translation="MRTIQIQQFPKNTTPTPIEFAINGDGAHQLNYIASYVPLRADAT
                     IGRADATVDFTVQYE"
     gene            5682..7373
                     /gene="eptB"
                     /locus_tag="ACHKFK_003410"
     CDS             5682..7373
                     /gene="eptB"
                     /locus_tag="ACHKFK_003410"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO9053677.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GISAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPVNAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAREAAAN"
     gene            7465..7541
                     /locus_tag="ACHKFK_003411"
     tRNA            7465..7541
                     /locus_tag="ACHKFK_003411"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7499..7501,aa:Pro,seq:cgg)
     gene            8275..9882
                     /gene="dppA"
                     /locus_tag="ACHKFK_003412"
     CDS             8275..9882
                     /gene="dppA"
                     /locus_tag="ACHKFK_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO9053678.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPKIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(9944..10171)
                     /locus_tag="ACHKFK_003413"
     CDS             complement(9944..10171)
                     /locus_tag="ACHKFK_003413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053679.1"
                     /translation="MTRSLELRVCIAHKSIPALVEQGMKYCCRMALHAEAQRHRVCRL
                     LTKSALFMPDAAQTPYPTYKIVQTQYIAEFS"
     gene            10189..11208
                     /gene="dppB"
                     /locus_tag="ACHKFK_003414"
     CDS             10189..11208
                     /gene="dppB"
                     /locus_tag="ACHKFK_003414"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO9053680.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11218..12120
                     /gene="dppC"
                     /locus_tag="ACHKFK_003415"
     CDS             11218..12120
                     /gene="dppC"
                     /locus_tag="ACHKFK_003415"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO9053681.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12131..13114
                     /gene="dppD"
                     /locus_tag="ACHKFK_003416"
     CDS             12131..13114
                     /gene="dppD"
                     /locus_tag="ACHKFK_003416"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO9053682.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNLVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDKCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13111..14115
                     /gene="dppF"
                     /locus_tag="ACHKFK_003417"
     CDS             13111..14115
                     /gene="dppF"
                     /locus_tag="ACHKFK_003417"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO9053683.1"
                     /translation="MSTHEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSSLNPPPGCAFNARCRRRFGPCIQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14145..15416)
                     /locus_tag="ACHKFK_003418"
     CDS             complement(14145..15416)
                     /locus_tag="ACHKFK_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO9053684.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLFYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASDFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTRKINSLVLTLAICTFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     SQLEKLRGFKAWMILLYGILLCLSPLLKLIE"
     gene            15892..15999
                     /locus_tag="ACHKFK_003419"
     CDS             15892..15999
                     /locus_tag="ACHKFK_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9053685.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16375..16482
                     /locus_tag="ACHKFK_003420"
     CDS             16375..16482
                     /locus_tag="ACHKFK_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO9053686.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(16568..18247)
                     /gene="bcsG"
                     /locus_tag="ACHKFK_003421"
     CDS             complement(16568..18247)
                     /gene="bcsG"
                     /locus_tag="ACHKFK_003421"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO9053687.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLFVAWLFLSQWTRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDIPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSIFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQSELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQISGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQNKPYVRLNGGDWVPYPQ"
     gene            complement(18244..18435)
                     /gene="bcsF"
                     /locus_tag="ACHKFK_003422"
     CDS             complement(18244..18435)
                     /gene="bcsF"
                     /locus_tag="ACHKFK_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO9053688.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18432..20003)
                     /gene="bcsE"
                     /locus_tag="ACHKFK_003423"
     CDS             complement(18432..20003)
                     /gene="bcsE"
                     /locus_tag="ACHKFK_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO9053689.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPGKITLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINSGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGCWTLVQHQEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLAQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20276..20464
                     /gene="bcsR"
                     /locus_tag="ACHKFK_003424"
     CDS             20276..20464
                     /gene="bcsR"
                     /locus_tag="ACHKFK_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO9053690.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDILALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
BASE COUNT         5232 a         4912 c         5328 g         5005 t
ORIGIN      
        1 cccgttgtat gaacaacatc aagcatattt atctatataa atggagttat aaatgaaaaa
       61 ggttctgttt gccttatctg cgcttgcgct gacctctact tccgtattcg cagctgatgc
      121 aggcgacggt tctgttaaat tcaccggaga gattgttgac gccgcttgtg ttgtgtctcc
      181 agacactcaa aaacaagaag ttgttctggg ccaggtgaac aagtctgtgt ttaccactac
      241 tggtgataaa tccgcagcaa ctccgttcaa aatcaaactg gaaaactgcg atatctccac
      301 tttcaaaaat gtggagatca gctttaacgg tgttggcgac gcagacaaca gcaaactgat
      361 ttctgtaagc actgaaccag gtgccgcaac tggcgtgggt attggtattt atgataatac
      421 caatacgctg gttgatctga ataccggtaa atctgctact gtcctgaaag aaggccagac
      481 tgttctgtat tttaccgcta actatgtcgc taccaaaaat gcagtaacca tcggttacgg
      541 taatgccgaa gtcgacttca acctgactta caactaatcg atccagacgg aacgattatc
      601 tctcaatggc aacagattat ctgttgccat tattcacacc aatgactcag ggaccgaatc
      661 atgaaacgaa ttagccttgc cgttctggcg cttttaatga ccgcacaaca gtgttttgct
      721 ggcggcgtag ccctaagcac gacgcgcgtt atttatgatg gtggcaagaa agaagcgtcg
      781 ctgaccgttg aaaaccataa taaaaatgaa gagtttttga tccagtcatg gattgatgat
      841 gtgaatggaa ataaaaaaac gccgtttatt attacaccgc cattattcaa acttgatcct
      901 gataaaaata acgtcttacg tattgttaat attgaagata ccttacccaa agatcgtgaa
      961 tccgtttact ggattaatgt aaaagcgatt cctgcgaaaa gtgctgaaaa tgaaaataaa
     1021 aacgtcttac agatagccgt tcgcacccgt ttgaagttgt tctaccgtcc gaaaggcttg
     1081 ccgggaagca accttgaagc aatgaaacag ttacgatttc agcgtcaaaa taaccaaatt
     1141 gtggttaata acgactcagc ttataacctg acctttaatc agatgtccat taatggacaa
     1201 aagattgaac gggcgggaat ggtcctcgct aaaggaacgt taaacattaa ccttccagcc
     1261 ggtacaggca atgcgaacga agtgaaatac agcattatta atgatttcgg tgtcgccggt
     1321 gaaatgctga cgaagtcgat taactgatct ttttacaagg cgctttacta tgcattggat
     1381 gcatttacct cttgatcact acagagcgca tttttctttc tcactgttgg cattaactat
     1441 tgccagcgcg ttacctgcgt acggcggaaa attcaaccct aaatttcttg aaaatgttca
     1501 gggtattgat caacacgtcg atttgtcagt ttacgactct cctgtcggcc agcaaatacc
     1561 cggtaaatat cgggtatttg tgtttgtaaa cgaagaaaaa atggcttccc ggacgctgga
     1621 ttttagtact gccagcgaag cacaacgcaa agccagcggc gagtcgctta tgccttgcct
     1681 tagccgtgtt caactggaag aaatgggcgt gcgcgtcgac agctttccgg cactgaagat
     1741 attgccaccg gaagcctgcg tagcgtttga tgaaatcatt ccccaggcca ccagtcgttt
     1801 tgattttaat actcagacgt tgcatcttac ctttccacag gcagcaatga tgatgaccgc
     1861 tcgtggcacg gttgatcctt ctcgctggga cgaaggaata cctgcgctgt tactggacta
     1921 cagctttagt ggcagcaatg gtcgcaacga aggttccggt tcatctccag attcgaccag
     1981 tgacagttat tacctgaacc tgcgcagcgg tttgaacgtg gggccgtggc gtttacgtaa
     2041 taacagtatc tggaaccgta ccgatggcaa aaatcaatgg gataacgttg gtacatcgtt
     2101 aaaccgggcg attattccac tcaagtcaca gataacattg ggtgataccg ccacgcctgg
     2161 cgagattttt gatagcgtac aaatgcgcgg ggcattattg gcatccgacg atgaaatgtt
     2221 gccggatagc cagcgtggct ttgctccggt ggtgcgtggc atcgccaaaa gtaacgctga
     2281 agtgagtatc gaacagaacg gatatgttat ttatcgtacc tttgttcagc cgggcgcatt
     2341 tgagattaac gatctctacg caacctccgg cagcggtgac ttgacggtga tcattaaaga
     2401 atctgacgga agtgaacaac gttttattca accattttcg gcagtggcga ttttccagcg
     2461 tgaagggtat ctgaaataca gccttgctgc cggtgagtat cgtgccggta attacgacag
     2521 cggtaaaccg cgattcgggc aatttactgc catgtacggc ttgccgtggg gaatgaccgc
     2581 ctatggtggt gcgttgctat cggcagacta caacgcttta gctctggggc tggggaaaaa
     2641 cttcggcacc attggtgcgg tatcggtgga tgtcacccag gcgaaaagtc agttacgcaa
     2701 caatgaaaaa gatgaagggc agtcttatcg tttcctctat tcgaaaagtt ttgagggcgg
     2761 taccgacctg cgtctgttgg gctacaagta ctcaaccagc ggttattaca ccttccagga
     2821 agcgaccgat gtccgtagtg acgccgacag tgactatcgg cgttatcata agcgcagcca
     2881 gatacaaggt aacatcactc agcaactggg agactacggt tctgtttact tcaatatgac
     2941 gcagcaggat tactggaacg ttgatggtaa ggagaactcc ttaagcgctg gttatcacgg
     3001 tcatattggt cgtgtgaatt acagtatcgc ctatacctgg acgagaagcc ctgaatggga
     3061 tgaagacgat cgactgtggt catttagcct ctcaataccg ctgggtggcg catggggcag
     3121 ttatcgtatg accaccgacc agaacggcaa gacgtcacaa caggcctctg tcagcggcac
     3181 attactggaa gatcgtaact tgaactataa cgttcagcag ggttatacca gtaatggggt
     3241 tggcaacagt ggtagtgtca acatgggtta tatgggaggt tctggcaaca ttgacgtggg
     3301 ttataactat tccaaagata accaacaagt taactatggt gtgcgtggtg gagtcattgt
     3361 tcacagtgaa ggtatcactc tctcgcaacc cttgggtgaa agtctggcaa ttgtttctgc
     3421 gccgggtgcg cgtggtggac atgtagtaaa tagcagcggc gttgaagttg actggatggg
     3481 caacgcagtg gtgccgtatc tcacgccata tcgtgaaacc attgtggaat tgcgcagcga
     3541 cacgttaggg caaaacgttg aattacagga agcgtttcag aaagtcgttc caacgcgtgg
     3601 ggcgatagtc cgttcccgct ttgatacgcg tgtcggttat cgtgtgctga tgagtttgaa
     3661 acgggcgaat ggcaacgcag taccgtttgg tgctaccgca gcgttaagtg atgagagcaa
     3721 acctgccagc agcattgtgg gtgaagaagg acagctttat atcagcggga tgccagaaga
     3781 gggcgaattg caggtgagct gggggcacga gcaggcgcaa cgttgccgcg tgccattccg
     3841 cttgccagag aaaaaggata attcgggtat cgtgatggtt aatgcggtat gcgataaata
     3901 aggatatgca gatgaaaatt aaaaacttgt tggcaggtat ggtgctttta gggacgagca
     3961 cctttaccgg gaatatatgg gctgcggatt gggggccgtg caggacggca agtggtgacc
     4021 cctttatttt tgttacatca ttcaccaaaa atattcaaaa tccgacggat aatgttaccg
     4081 ggcagaccta ccctgatttt taccaatggg cactgggtga taaatattct ggtgtttgtg
     4141 aatgtccatc accgaacccg acagaagcac gaccaacgct atataaaact gagtcaacat
     4201 tggcggcggg gcataactca acctatttta aaattaccaa taatcttgag gtttcaaccc
     4261 gggtatatat tgcaaacgtg gggaatgtac aggttccatt tatcaataaa tcaaatagcc
     4321 agccaggaag agagtgcgat caaccaactt ttggctggac tacagggagt aaaggacagc
     4381 tctcacttta tatcgctaaa ccttttgttg gggaacaaaa cattccgcaa actattatag
     4441 tttctgtgtt tggtacaaaa aaggagaatg tttatagttc agttccaata tcacaggtgc
     4501 ttctttcagg aaaggtcacc gtaactcaag gctgtgaact tgccgcaggg acatcattgg
     4561 atatcgactt tggtgagtat caggcccatg atttcaaagg tcgaaccggg cagccgccac
     4621 aaaatgtcca gaaaatacaa aaagaattaa cgtttaactg tactaatatt tctgatggtg
     4681 tccatatcta tctgagtctt gaaggtacgc caaacgcggc ttaccccagc gcaatcagtt
     4741 tgggcaacgc ggatgttggt gcggtaattg aggatggtaa aggtaatatt cttaagccga
     4801 atgatagtaa tagcttatta gaaatgaatc ccggctcact gtacgaatat gtcaaacgta
     4861 aagtaacaac caccattact gcttatccgg ttagtacaac cgggaaatta cccgcagccg
     4921 gtgattatag cggtgtggca acaatgcatg ttgaattgga ttagcactga tgaaacgaac
     4981 aatacttgca ttatgcctgc tacctttttc cttttcgtca ctgtcggcaa ccacagactt
     5041 aggggcgaaa ggaacgttga aattctctct taaaatcagc cagggagcct gtgaactggt
     5101 aaaagacagt gttgaagttg acatgggatt agcggttctc aaaaagccag taagaatcgg
     5161 aacagaactt aatcccacta ctttttccat cggtctgaaa aactgtagcg aagttgtcag
     5221 ggcttacgtc accatggatg gaacacccaa tgcggacgat ccaaatacaa cagttcccga
     5281 aaaatacgac accgacgccg attgagttcg cgattaacgg tgatggcgcg catcaactga
     5341 actatatcgc cagctacgtg ccgctgcgtg ctgatgcaac cattggccgc gcagatgcga
     5401 cggtcgattt tacggttcag tacgaataat ttcgcttcac cggggctagc tcactggccc
     5461 tttttactgc tttttgtaac gctaaagtct cttttcaaac ttgcattttt gtaaatttgt
     5521 gcttcgtgca cactctttcc ccacactttt tccctttgct gtggtctact tattcgcgcg
     5581 tgtagatttt acttatctga ctacctccgc acttttcccc tgcctggcct gaaaagccac
     5641 taagcagggt gttatcacct gtttgtccag ggcttgtttg catgagatac atcaaatcga
     5701 ttacacagca gaagctgagc ttcttgcttg caatctatat tggccttttt atgaatggcg
     5761 cggtttttta ccgccgcttc ggcagctatg cgcacgattt taccgtctgg aaaggtattt
     5821 ctgctgttgt tgaactggcc gccaccgtac tggtgacctt ctttttactg cgtcttcttt
     5881 cgctgtttgg ccgccgcagc tggcgtattc tggcatcgct ggtggtgctc ttttccgcag
     5941 gtgccagcta ttacatgacc ttccttaatg tagtcattgg ttatggcatc atcgcatccg
     6001 tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg tctgaacttt attctctggt
     6061 taatcgccgt aagcgcgttg cctcttatcc ttatctggaa taaccgctgt cgttacacct
     6121 tgctccgaca actgcgaacc ccagggcaac gtattcgcag cctggcggtc gtcgtactgg
     6181 caggtattat ggtttgggca ccgattcgtt tgctggatat ccagcagaag aaagtggaga
     6241 gggcgacagg tgttgatttg ccgagttatg gcggcgtcgt cgcgaactct tatctgccat
     6301 caaactggct ttcggcatta ggtctttatg cctgggcgcg ggtcgatgaa tcttccgata
     6361 acaattctct ccttaaccct gcgaagaagt ttacgtacca ggctccacaa aacgttgatg
     6421 atacatatgt cgttttcatt attggtgaaa ccacgcgctg ggatcatatg ggcattttcg
     6481 gctatgaacg caataccaca ccgaagctgg ctcaggagaa aaatctggcg gcgttccgtg
     6541 gttactcctg tgatactgca accaaactct cactgcgttg catgtttgta cgtcagggtg
     6601 gagcagaaga taatccgcag cgcacattaa aagaacagaa cattttcgcg gtattgaagc
     6661 agttaggatt cagttctgat ctctacgcta tgcaaagcga aatgtggttc tacagcaata
     6721 cgatggcgga caacattgct tatcgtgagc agattggtgc ggagccacgt aaccgtggca
     6781 agccggtaga tgatatgttg ctggtagacg aaatgcagca atcgctgggg cgcaacccgg
     6841 atggtaagca tctgatcatt ctgcatacca aaggctcaca ctttaactac acccagcgtt
     6901 acccgcgcag ctttgcgcag tggaagccgg aatgtattgg tgttgatagc ggctgtacca
     6961 aagcgcagat gatcaactca tacgacaact cggtgaccta tgtggatcac tttatttcca
     7021 gcgtgattga tcaggttcgc gataagaaag cgattgtgtt ctatgcagct gaccacggtg
     7081 agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg tgaactggca ccgccggaac
     7141 agttccgtgt gccgatgatg gtctggatgt cggataaata tctggaaaat ccggtcaatg
     7201 cgcaggcatt tgcgcagctg aaaaaagaag ccgacatgaa agtgccacgc cgtcatgtag
     7261 agctgtacga caccatcatg ggttgtcttg gctatacttc accggatggt ggaattaacg
     7321 aaaacaacaa ctggtgccac atcccgcagg caagagaggc ggcggctaac taagaggcct
     7381 gctgactttc tcgccgatca aaaggcattt tgctattaag ggattgacga gggcgtatct
     7441 gcgcagtaag atgcgccccg cattcggtga ttggcgcagc ctggtagcgc acttcgttcg
     7501 ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc aaattcgaaa agcctgctca
     7561 acgagcaggc ttttttgcat ctgtaactca tgaggaagag aacctccggg ggcggggagg
     7621 ttcgactcga cgtaggcctg ataagacgcg ccagcgtcgc atcaggcaaa tgtaccggcc
     7681 cattgtcgga tgcggcgcga ccgccttatc cgatctacac accacacatt tccctgccaa
     7741 atttgcgccc catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt
     7801 ttatatttgc ttaaatcttt ttttgcgtta cttatggctg gcgctggcat tttccgctgt
     7861 gcgttttagc gttcatggta ttaatcgctc agatatccac ctttacctca gatttttttc
     7921 attaaatgca taaaacttaa tcaccaaatg ttgctaaata ttaagctgat tgttctggtt
     7981 gtgttggcgt agcacaaatt tctctgctgc aaatagctgt tttaatcttt gtttgtggat
     8041 tctcaccgtt ggtgtaaatc ccggttggct gtattgacgt tttcacattc tgttgacaga
     8101 ttgtaggtca cgaggggcat tttatggagg atccgcactg ttacactgat gttaattagt
     8161 acggcatccc cacctcataa cgttgacccg accgggcaaa aaacaaaaaa ggtcaggcag
     8221 cgacaaccca ctgcaaaggg ttaaaacaac aaacatcaca attggagcag aataatgcgt
     8281 atttccttga aaaagtcagg gatgctgaag cttggtctca gcttggtggc tatgaccgtc
     8341 gcagcaagtg ttcaggctaa aactctggtt tattgctcag aaggatctcc ggaagggttt
     8401 aacccgcagc tgtttacctc cggcaccacc tatgacgcct cttccgtacc gctttataac
     8461 cgtctggttg aatttaaaat cggcaccact gaagtgatcc cgggcctcgc tgaaaagtgg
     8521 gaagtcagcg aagacggtaa aacctatacc ttccatctgc gtaaaggtgt gaagtggcac
     8581 gacaataaag aattcaaacc gacgcgcgaa ctgaatgccg atgacgtagt gttctcgttc
     8641 gatcgtcaga aaaacgcgca aaacccgtac cataaagtct ctggcggcag ctacgaatac
     8701 ttcgaaggca tgggcttacc agagctgatc agcgaagtga aaaaggtgga cgataacacc
     8761 gttcagtttg tgctgactcg cccggaagcg ccgttcctcg ctgacctggc aatggacttc
     8821 gcctctattc tgtcaaaaga atatgctgac gcgatgatga aagccggtac accggaaaaa
     8881 ctggacctca acccaatcgg taccggcccg ttccagttac agcagtacca gaaagattcc
     8941 cgtattcgct ataaagcgtt tgatggctac tggggcacca aaccgaagat cgatacgctg
     9001 gtcttctcta ttactcctga cgcttccgtg cgttacgcga aattgcagaa gaacgaatgc
     9061 caggtgatgc cgtacccgaa cccggcagat atcgcccgca tgaagcagga taaatccatc
     9121 aacctgatgg aaatgccggg gctgaacgtc ggttatctct cgtataacgt gcagaaaaaa
     9181 ccactggatg acgtgaaagt tcgccaggct ctgacctacg cggtgaacaa agacgccatc
     9241 atcaaagcgg tttatcaggg cgcgggcgta tcagcgaaaa acctgatccc gccaaccatg
     9301 tggggctata acgacgacgt tcaggactac acttacgatc ctgaaaaagc gaaagccttg
     9361 ctgaaagaag cgggtctgga aaaaggtttc tccatcgacc tgtgggcaat gccggtacaa
     9421 cgtccgtata acccgaatgc tcgccgcatg gcggagatga ttcaggcaga ctgggcgaaa
     9481 gtgggcgtgc aggccaaaat cgtcacctac gaatggggtg aatacctcaa gcgtgcgaaa
     9541 gatggcgagc atcagacggt aatgatgggc tggactggcg ataacgggga tccggataac
     9601 ttcttcgcca ccctgttcag ctgcgccgcc tctgaacaag gctccaacta ctcaaaatgg
     9661 tgctacaaac cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga ccacaataaa
     9721 cgtgttgaac tgtacaaaca agcacaggtc gtgatgcacg atcaggctcc ggcactgatc
     9781 atcgctcact ccaccgtgtt tgaaccggta cgcaaagaag ttaaaggcta tgtggttgat
     9841 ccattaggca aacatcactt cgaaaatgtc tctatcgaat aattaaagcc atgcaagact
     9901 gatggcaaag gcaaaactgc ctgatgcgct ccgcttatca ggcctacgaa aactctgcaa
     9961 tgtattgagt ttgcacgatt ttgtaggtcg gataaggcgt ttgcgccgca tccggcataa
    10021 acaaagcgct ctttgtcaac aatctgcata cccggtggcg ctgtgcctct gcgtgcagcg
    10081 ccatccggca gcaatacttc attccctgtt ccacaagggc gggaattgat ttgtgagcaa
    10141 tacagacacg cagttccagg ctgcgggtca ttatagagaa tccgggttat gttgcagttt
    10201 attctccgac gtttgggact cgtcatcccc acgtttatcg gtattaccct tctcacattt
    10261 gcctttgtcc acatgatccc gggcgatccg gtgatgatca tggcgggcga acgtgggatc
    10321 tccccagagc gtcacgcgca attgctggct gaactcggct tagataaacc gatgtggcag
    10381 cagtatctcc attacatttg gggcgtaatg cacggtgatt taggcatttc gatgaaaagc
    10441 cgaattccgg tatgggaaga gttcgtgccg cgctttcagg ccacgctgga acttggcgtc
    10501 tgcgcgatga tttttgcgac ggcagtcggt attccggttg gtgtgctggc tgcggttaaa
    10561 cgtggttcca ttttcgatca cacagcggtt ggcctggcgc tgaccggcta ctcgatgcca
    10621 attttctggt ggggcatgat gctgatcatg ctggtttcgg tgcactggaa cctgacgcct
    10681 gtctccggtc gcgtgagcga catggtgttc ctcgatgatt ccaatccgtt aaccggtttt
    10741 atgctgatcg acaccgccat ctggggtgaa gacggtaact ttatcgatgc cgtcgcccat
    10801 atgatcctgc ccgccattgt gctgggtact attccgctgg cggtcattgt gcgtatgacg
    10861 cgctcctcga tgctggaagt gctgggcgaa gattatatcc gcaccgcgcg tgccaaaggt
    10921 cttacccgga tgcgggtgat tatcgtccat gcgctgcgta acgcgatgct gccggtggtg
    10981 accgttatcg gcctgcaggt gggaacattg ctggcggggg cgattctgac tgaaaccatc
    11041 ttctcgtggc ccggtctggg gcgctggttg attgacgcac tgcaacgccg cgattatccg
    11101 gtagtgcagg gcggcgtatt gctggtggcg acgatgatta tcctcgtcaa cctgctggtc
    11161 gatctgctgt acggcgtggt gaacccgcgt attcgtcata agaagtaagg ggacatcatg
    11221 tcacaggtta ctgaaaataa agtgattagc gcaccggtgc cgatgacccc gttacaggag
    11281 ttctggcact attttaaacg caacaaaggc gcggttgtcg ggctggttta cgtcgtcatc
    11341 gtgctgttta tcgcgatctt tgccaactgg attgcaccct ataacccagc ggaacagttc
    11401 cgcgatgcac tgctcgcccc gccagcctgg caggaaggcg gcagcatggc gcacttgctg
    11461 ggcactgatg acgtggggcg tgatgtgctg tcgcggctga tgtacggcgc gcgcttgtct
    11521 ctgctggttg gctgtctggt agtggtgtta tcgctgatta tgggcgttat tctcggcctg
    11581 atcgctggtt actttggcgg tctggtcgat aacatcatca tgcgcgtggt cgatatcatg
    11641 ctggcgctgc caagtctact gctggcgctg gtgctggtgg cgattttcgg cccgtcgatt
    11701 ggtaacgccg cgctggcgct aaccttcgtt gcgctgccgc attatgtgcg cttaacccgc
    11761 gccgccgtgc tggtggaagt taaccgcgat tacgtcaccg cgtctcgcgt ggcgggtgcc
    11821 ggggcaatgc gccagatgtt tattaacatc ttcccgaact gccttgcgcc gctgattgtt
    11881 caggcgtcgc tcggtttctc taacgccatt ctcgatatgg ctgctcttgg tttcctcggc
    11941 atgggcgcgc agccgccaac gccggagtgg ggcaccatgc tctctgatgt gttgcagttc
    12001 gcgcaaagcg cctggtgggt cgtgaccttc ccgggtctgg cgatcctgct gacggtgctg
    12061 gcatttaacc tgatgggtga cggtctgcgt gacgcgctcg atcccaaact gaagcagtaa
    12121 gaggttcgag atggcgttat taaatgtaga taaattatcg gtgcatttcg gcgacgaaag
    12181 cgcaccgttc cgcgccgtag accgcatcag ctacagcgta aaacagggtg aagtggtcgg
    12241 gattgtgggt gagtccggct ctggtaaatc ggtcagttcg ctggcaatta tggggctgat
    12301 tgattatccg ggccgcgtaa tggcggaaaa gctggagttt aacggtcagg atttgcagcg
    12361 tatctcagaa aaagagcgcc gcaacctggt gggtgccgaa gtggcaatga tcttccagga
    12421 cccgatgacc agccttaacc cgtgctacac cgtgggtttc cagattatgg aagcgattaa
    12481 ggtgcatcag ggcggcaaca aaagtacccg ccgtcagcga gcgattgacc tgctgaatct
    12541 ggtcggtatt cccgatccgg catcgcgtct ggatgtttac ccgcatcagc tttccggcgg
    12601 catgagccag cgcgtgatga tcgccatggc gattgcctgt cgaccaaaac tgctgattgc
    12661 cgatgaaccg accactgcgc tggacgtgac catccaggcg caaatcatcg aactgctgct
    12721 ggagttgcag cagaaagaga acatggcgct ggtgttgatc actcatgacc tggcgctggt
    12781 ggcggaagcg gcacataaaa tcatcgtgat gtatgcaggc caggtagtag aaacgggcga
    12841 tgcgcacgct atcttccatg cgccgcgtca cccgtatact caggcattgc tgcgtgcgct
    12901 gccggaattt gctcaggaca aagaacgtct ggcgtcgttg ccaggtgtcg ttcccggcaa
    12961 gtacgaccgc ccgaacggct gcctgcttaa cccgcgctgc ccctacgcca ctgacaaatg
    13021 ccgtgctgaa gaaccggcgc tgaatatgct cgctgacggg cgtcagtcca aatgccatta
    13081 cccacttgat gatgccggga ggccgacact atgagtacgc atgaggccac ctcgcaacaa
    13141 ccgctgttgc aggctatcga cctgaaaaaa cattatccgg tgaagaaagg catgttcgcg
    13201 ccggaacgtc tggtgaaggc gctggatggc gtttcgttta accttgaacg tggcaaaacg
    13261 ctggcagtag taggtgaatc tggctgcggt aaatcgaccc tcggtcggtt gctgacgatg
    13321 attgaaacgc ccaccggcgg cgaactgtat taccaggggc aggatctgct caagcacgat
    13381 ccgcaggcgc agaagctacg tcggcagaaa atccagattg tcttccagaa cccttacggt
    13441 tcgctgaatc cgcgtaaaaa agttgggcag attctcgaag agccgctgct gatcaacacc
    13501 agcttaagca aagaacagcg tcgggaaaaa gccctgtcga tgatggcgaa agtcggcctg
    13561 aaaaccgagc attacgaccg ctatccgcat atgttctccg gcggtcagcg tcagcgtatc
    13621 gccattgccc gaggtctgat gctcgacccg gatgtggtga ttgccgatga gccggtttct
    13681 gcgctggacg tctcagtgcg tgcgcaggtg ctgaatctga tgatggattt gcagcaggaa
    13741 ctggggctat cttatgtctt tatctcgcac gatctgtcgg tggtggagca cattgctgat
    13801 gaagtgatgg tgatgtacct gggccgctgc gtggagaagg ggacgaagga ccaaatcttc
    13861 aataacccgc gccacccgta cactcaggca ttgctctccg cgacaccacg cctgaacccg
    13921 gacgatcgtc gcgaacgcat caaacttacc ggtgaactgc caagctcgct gaatccaccg
    13981 ccaggctgcg ccttcaacgc ccgctgtcgt cggcgcttcg gcccctgcat ccagttgcag
    14041 ccgcagctaa aagactacgg cggtcaactg gtagcctgtt ttgccgttga tcaggatgaa
    14101 aatccgcagc gttaactcac aaaaccgggg ccatgcgctc cggtttactc aatcaacttc
    14161 aacagtggcg acaagcataa caaaatgccg tacagcagaa tcatccaggc tttaaacccg
    14221 cgaagttttt ccagttgtga gactttatag atcaggaaaa acggaatgag gcacgacaca
    14281 atgccatata acgggcttcc caactgaaag aacaccagta ccgatacacg aaacgaaacc
    14341 caaatcgtca acgtaatgac gataaaagtg cagatcgcca gagtcagcac gagtgagtta
    14401 attttcctgg tatcaataat tcggcttaac agattgagaa taatgccttt aatggcctcg
    14461 tggaaaccga gataaatgcc aaagaatgca gtcagtacgg caaagatatt cagcaccgta
    14521 gaggtgatat gaatgatatg cccagggatc acctgcgcgg ccagtgccag tgccgagata
    14581 ttttgttcaa aggcagaaac ggcttcttcg tggctaattg agaaggtaaa cgaaaaggca
    14641 aaaaacagga tcaccgcgat gagcgtgaca taactaattc ggtgggtacg cagcgcgagc
    14701 cgggttgcca gtactttatc cgcttcccgt ttacgatagg caatattcat tgggttaagt
    14761 acctgaataa ataccgcgga aaagaagcaa aatggaatag taagcaggac atcgcggaaa
    14821 aagtcggagg cttgcgggaa ggcggttata ttggcgaaat tccagtgcgg gatcatcgca
    14881 aaaccgaaca cgacaataat ccctactttg accaccacca ttggtccgga aatcttaaac
    14941 agtaatcgtt caccaccaga cgcaatcgcc accagtacgg cgaaaatggc gactttataa
    15001 aatagagatt gagaaagatc ggcatcggtt aaaccgaagg ttttcaggta cgaggcgctg
    15061 tcgaaaacca cggagagaga gtagataaaa atcccgtgga taatcatcaa aaagtagata
    15121 accccgagga aaattcccca gttcttcccc aggtaatggc tgataatatc ggtgtagtca
    15181 ttacaggaat cgctttcaga aagggttttt aaataaatgt cctgtactac ccaggtggca
    15241 ggataagcaa tgatcgctgc ggtaataaat acccaaattc ccttcaagcc aatttgtact
    15301 ggcatcagca cggttccggc accaatcgcc atgcctatgc ataataaaac ccagccgaag
    15361 tcgtagcgtg taaacggcaa tttctgatcg tgtttcggta gtgtgttgtg ctgcataaag
    15421 tcctgtccag aaagaagtcg gcctcgcaaa aatgaataaa cccagtttct cttgcgcaac
    15481 aggtggagaa gtcgaatacg cagtatgtaa aaaatgaggc aacagattca ctggggaggt
    15541 cgcgaggagg cgcaattcta caaaattgcg catttcacta ctagtctgga gtcggcccgg
    15601 atatgagctg caactattgc aaataaagaa attaagctgg aaggcatcac gcaatgagga
    15661 agattgccgg gtgctgacgg caaaaagcaa tcgtaaaatc agcggcaagc cggaagggtt
    15721 ccggtgaggc gcaatgttgc gggggcttta tccctggtgg cattggttgc tggagagaga
    15781 aaacccccgc acgttgcagg tatgcacctg acaacatcac gggggccatt cttgcatcta
    15841 gaccattcaa gaatagccgc gaaacgttgt cattacaaca caggcggcta tatgacgctc
    15901 gcagagctgg gcatggcctt ctggcatgat ttagcggctc cggtcattgc tggcattctt
    15961 gccagtatga tcgtgaactg gctgaacaag cggaagtaac gtgtcatgcg ggcgtcaggc
    16021 tgccgtaatg gcaatttgcg cccggaccag gccgcagagg tgaaactctg cggccttttt
    16081 cgtaagaaaa atgcaacggt tgcaagcgga taatattctg cggaatgcgc agcacagagt
    16141 gaagaagtga cggattccgg agcagaaaaa cagtcgtaga atcagcggca agccggaaag
    16201 gttccggtga ggcgcaatgt tgcgggggct taatccctgg tggcattggt tgctggagag
    16261 agaaaacccc cgcacgttgc aggtatgcac ctgacaacat cacgggggct aatcttgaac
    16321 ctagaccact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg ctatatgacg
    16381 ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggcatt
    16441 cttgccagta tgatcgtgaa ctggctgaat aagcggaagt aacgtgtcat gcgggcgtca
    16501 ggctgccgta atggcaattt gcgcccggat caggccgcag ggggaaactc tgcggccttt
    16561 ttcgttctta ctgcgggtaa ggcacccagt cgccgccgtt caggcgaacg tacggtttat
    16621 tctggtattg aataactact gcatttgagt tctcggagac cggtgctgtt tgtggtaacc
    16681 cactggtgag ttttttccag tcaacattgt cttcggtgaa aatcttgcca tcaagaacgc
    16741 gaaccaccag atcggagata gccaggaagc tgctcggttg gtcgatgaca atcggagccc
    16801 cctgatgtgg tgccttcatg ccgaagaatt tcaccccaac ggggacgtct gtgatagacg
    16861 ggctagggat atcacgtaga ccagatatct gcattctgtc gcccttcagc gcgccgccgt
    16921 gttccggcac cactaccacc atcactttac gacccgattt ctccagttca gtaaagaagg
    16981 cgtccagttc atcaaagaat ttttgcgccc gcgctttgta atccgctgtt ttgctgacgc
    17041 ccggataatg gttgccgtca tgcagtggaa gcgtgttgta gaacgtggca ctacggctgt
    17101 ttttatcttt ttcggtaacg tccagccaac ggttaagcac ggcggtatcg tcataaaccg
    17161 gcgaaccatc aaagcccagc aaaataaccg gcagatttgt ttgatccatc aattcgctct
    17221 gcatgccgcc attttcgcga acttccttca aaaaaccacc gaactggccg ttatgtccca
    17281 tcatcaggtg ctgggtaaag cccagtttcg acaggttatc aaacagatag cagtcgttat
    17341 ttgccggttg atacagatta gtgtgcgaag tctgcccgca gctggcgcgc agtaaacgga
    17401 tcgccgccgg gccactgtag gaggtggcgg agttaaagtt cttgaactca atatcgaaat
    17461 gcgaccacag tggatgcgac atcaatccgg cggcttcaat atccgaccag gaaagcgaac
    17521 agatattaat taccagtagt tcaaatggct gagcatcagc gggcagcgaa gacgggaaga
    17581 tcgatttacg tttcgcctcc gcgttataga aattattcag ccaggcgtta aggtttgctg
    17641 ttgttggcgg tgcagtttgt gcgggtatat cacccactac cggtgcgcca cccgtcgccg
    17701 caacggttgc cgctgcgtta ccacccgtcg ttgttacagt ggtcgtcggt tgaccggctg
    17761 gccacaagga gaaacttggt cccgccaggg taagtacgtt cagccatagc agaatggcaa
    17821 ccacaaaaac ggtaatgcga gtccattgtg acaggaataa ccaggccacg aataaaacaa
    17881 aaatggcccc aatcatctgc cagttaataa agcgtgtgac aagatcgatt aaataatcgg
    17941 tactgaaccc cgccacctgc gaaccctggc tcattatgct ttccgggcca ggcaaccagg
    18001 tgtcatgcca gaacaaagca aagccgatcg gcagggcaat ccagtggcgc aagcgatgca
    18061 ggctgtagcg cggaatgggc atcagcagaa acgcggcaaa caccagattg aggagcggat
    18121 ggaagttaag atatcccgcc cacaacaggc cgaacttaac cagaaaatag aagttccagc
    18181 cggaaaggcc gcgccagtat tgccagaggg aagaaggcat ggcggtattt tgcgtaaatt
    18241 gagtcatttt ttggttgccc tggctttttc cgtgcggcgt aacgtcccgg ccggtttaac
    18301 ataacgaggt ttagcaaaga acaaacgtaa ggtgtcgcga atgcgtcgca aagagtgccg
    18361 cgccagatag cccagcggga aaaatatcag tgcgcaaacg acaataattt caatgatatc
    18421 gctgatggtc atcatgatga gcgctccaca gcatcatcta acagtcgcat tggttctggt
    18481 attcgtcgcc agtggcgacc atcgtgctcg gcattgatga ccggtttaga actttgcgct
    18541 aaaggcagcg gcatgcccca ttgttctggg gcaagcaggc gcatctgcac cagctcggca
    18601 ctgatttgat catcttcaaa ccagaccatt cggtttgaga aaatgtcgcc agtaggcaat
    18661 gggaaaatat gattcaacgc ggtatccaga tcgttaatcc gacagaatga gagaaacagc
    18721 accagccgat taccgccaat ggtcatgata tcgccggtac ggttagggcg acatagcgtc
    18781 agggcttgct caacgcggat acccggtacc ggacgtaggg caaccagaac gcctttaccg
    18841 tgggcaggta atagagggtt attcatcatg ttgttgacgg cattacagaa cacatcccac
    18901 ttctggaaac cacgcagttt gagcggctgg gtcattgaca gcaaggtagt gatatcttcc
    18961 ggcacatagc gactaaactt ctgcccttgc acgctttcga tcatcgtcag acagcgggag
    19021 agtggcgcat tccacgggat aaccatattt gcaccgcagg ccaataataa acgttcatcg
    19081 gtggcgcgca ggctggcggt attttcccgt acgaggattt tcatcgcact accgcgctgg
    19141 cgacgcagag tatgaatgct gcgggccagt ggctcgattt gcgcattttg ctgtaaagaa
    19201 aagaccaccg tcgccgcctg agcggtacgg gcttcattga acaggacttc gttattgttg
    19261 aacagttgcc agtgttccga tagcggcggc gcaccttcca gtacagcaac attactcaga
    19321 atgcgttttt cgtcgctgcg tggttggatt tccgcctcct ggtgttgaac taatgtccag
    19381 caaccatttt gttgctgaac gctaagctgc tgacgggcgc tgaccccttt ttcgttgcac
    19441 cagaaagcaa tatccagcaa atgttggtcg ccctgaaaac gcaaactggc aagaccaaaa
    19501 agtgaccggt attcctcaag taacaatgaa aattgtttat cgttattatt tccggaatta
    19561 attaccaaaa gcgaacaatg gtttagccgg ctccatttat tcattttatc caaccatgag
    19621 cgaagccgct cggcaggaat gttttgccat gcgttatttg cgcaaacaag aataaaaagg
    19681 taattatggg gatcaataga acactgcaaa tcacgggcca aatagtatag acctttttca
    19741 tgatttagca ttgaaaataa tgttattttt cccggccctt gagaatcatc taattgaaag
    19801 attttcgctg gatcgctgtc catgctaatg accgcgacgt gtgcggtttc agcctgggat
    19861 gcaattgttt gattcgccag actgatagca tcttcatggc gatcgacgtt aaaccaccag
    19921 acgccgcctg ctggcatatg tcgcagctca tcccataatg atgagatacc gatagagaat
    19981 acagggtcca caatgtccct catgtcaaca ttttgtctga ttatcagttt actagcgaaa
    20041 gctaagaaat aaacctacca ttgaaattaa aacttatcag atttagcatg tgaatcaatt
    20101 tagcaggtca caagacatgt gattttagtc ttttcgtttt taactttttc tgttgcgtaa
    20161 cggaaagtca aaaagtgagc acattcccgt ctcgccgtaa acaataaacc gacgaaatgc
    20221 tcacagttag aattgtctca tgaacggtac ggttatttca tagggatcaa gaaaaatgaa
    20281 taacaatgaa ccagatactc tgcctgatcc cgcgataggc tatatcttcc agaatgatat
    20341 tttggcgtta aagcaggcat tttcactgcc tgatattgat tatgccgata tttcccaacg
    20401 cgaacagttg gccgcggcat taaaacgctg gccgttgctg gcagagtttg cgcaacaaaa
    20461 gtaggggatt ggtgaat
//
LOCUS       DAZGJT010000084        20285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZGJT010000084 DAZGJT010000000
VERSION     DAZGJT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.084"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(419..1615)
                     /gene="hemY"
                     /locus_tag="ACHKFK_003425"
     CDS             complement(419..1615)
                     /gene="hemY"
                     /locus_tag="ACHKFK_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO9053691.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1618..2841)
                     /gene="hemX"
                     /locus_tag="ACHKFK_003426"
     CDS             complement(1618..2841)
                     /gene="hemX"
                     /locus_tag="ACHKFK_003426"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO9053692.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSTVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAVQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPQADAPAAAPQGE"
     gene            complement(2863..3603)
                     /gene="hemD"
                     /locus_tag="ACHKFK_003427"
     CDS             complement(2863..3603)
                     /gene="hemD"
                     /locus_tag="ACHKFK_003427"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO9053693.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDSLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSRKVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3600..4541)
                     /gene="hemC"
                     /locus_tag="ACHKFK_003428"
     CDS             complement(3600..4541)
                     /gene="hemC"
                     /locus_tag="ACHKFK_003428"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO9053694.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICKRE
                     DPRDAFVSNTYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4928..7474
                     /gene="cyaA"
                     /locus_tag="ACHKFK_003429"
     CDS             4928..7474
                     /gene="cyaA"
                     /locus_tag="ACHKFK_003429"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO9053695.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRREVLSQLVKEWEWDDSRLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQEHDAPLLQQYFS"
     gene            complement(7514..7834)
                     /gene="cyaY"
                     /locus_tag="ACHKFK_003430"
     CDS             complement(7514..7834)
                     /gene="cyaY"
                     /locus_tag="ACHKFK_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO9053696.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7882..9198)
                     /locus_tag="ACHKFK_003431"
     CDS             complement(7882..9198)
                     /locus_tag="ACHKFK_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9053697.1"
                     /translation="MNGQIITVALILLGNTFLTPPVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMNRADLRLVKNISGKTGYIPSDEISIENEEYHWVTDGHVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGAH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPEQFHLITDNIAQYKDRLYVTKLSTFGEDQLEIIPLDTP
                     DLVIDDSFNSGKQHAYFIRQLRSRKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVR
                     ITPSHCPAKTRVREENVREIHNSDIIIPVTDESCRNAAADGQTLKP"
     gene            complement(9195..10664)
                     /locus_tag="ACHKFK_003432"
     CDS             complement(9195..10664)
                     /locus_tag="ACHKFK_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO9053698.1"
                     /translation="MHYRNVVCLLSCSLFLSSAWGCRLDEPEHNIYQKQGKGVVYLRP
                     YEKTNLSLPQINYKRLRLLPNLLIDPTKLEDWETVPPATNLMTDVVYSGANATLPHYS
                     YYSDGRAILYAGEIVQNPPGTPPVDISSFQAWGDFAADKYSLYYEGKRTDSNQQLNRR
                     TLRQVEFNPQWKPDWLGLILRDKHYLYANGQRLDDPDTFTVLAQKSWDQRGKFSTAFN
                     PCLPAPFGPWDTLARTQTKIMINGEQLDADPDTFSVVRWMPGSLFTWRDKNGLQRKVL
                     DKENLAWDEDLTKHCLDFSLQEKKVFWRKGPACKQEELSGLDPEQFHPISDAVAQYQD
                     SLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAEGWVNHSSLHVFES
                     DGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYVYSFDGAQLWRYRTADPKQVRLI
                     WKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            10853..11056
                     /gene="yifL"
                     /locus_tag="ACHKFK_003433"
     CDS             10853..11056
                     /gene="yifL"
                     /locus_tag="ACHKFK_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO9053699.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDSATGDGPSQVNY"
     gene            11093..11917
                     /gene="dapF"
                     /locus_tag="ACHKFK_003434"
     CDS             11093..11917
                     /gene="dapF"
                     /locus_tag="ACHKFK_003434"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO9053700.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            11914..12621
                     /gene="yigA"
                     /locus_tag="ACHKFK_003435"
     CDS             11914..12621
                     /gene="yigA"
                     /locus_tag="ACHKFK_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HFO9053701.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLARWIERV"
     gene            12618..13514
                     /gene="xerC"
                     /locus_tag="ACHKFK_003436"
     CDS             12618..13514
                     /gene="xerC"
                     /locus_tag="ACHKFK_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO9053702.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIIHFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMSRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            13514..14230
                     /gene="yigB"
                     /locus_tag="ACHKFK_003437"
     CDS             13514..14230
                     /gene="yigB"
                     /locus_tag="ACHKFK_003437"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO9053703.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRVDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            14314..16476
                     /gene="uvrD"
                     /locus_tag="ACHKFK_003438"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             14314..16476
                     /gene="uvrD"
                     /locus_tag="ACHKFK_003438"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO9053704.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(16521..17423)
                     /locus_tag="ACHKFK_003439"
     CDS             complement(16521..17423)
                     /locus_tag="ACHKFK_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HFO9053705.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTERKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(17506..18270)
                     /gene="yigE"
                     /locus_tag="ACHKFK_003440"
     CDS             complement(17506..18270)
                     /gene="yigE"
                     /locus_tag="ACHKFK_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO9053706.1"
                     /translation="MAHRLFIGKGMITLNLKRIFLTLTLLPLFAVAADDCAPSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     LLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGEAIPWQRYPFVTMISVEQKG"
     gene            18432..18488
                     /gene="ysgD"
                     /locus_tag="ACHKFK_003441"
     CDS             18432..18488
                     /gene="ysgD"
                     /locus_tag="ACHKFK_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO9053707.1"
                     /translation="MDTPSRYWLIILSSRINS"
     gene            18640..19590
                     /gene="corA"
                     /locus_tag="ACHKFK_003442"
     CDS             18640..19590
                     /gene="corA"
                     /locus_tag="ACHKFK_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO9053708.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(19626..20123)
                     /locus_tag="ACHKFK_003443"
     CDS             complement(19626..20123)
                     /locus_tag="ACHKFK_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO9053709.1"
                     /translation="MKRKLQKIILYLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQDEIKSQNPLPEDSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHFFIADNNEYQLTQENKIERVFNPHDFEK
                     DNFQQ"
     gene            complement(20120..>20285)
                     /locus_tag="ACHKFK_003444"
     CDS             complement(20120..>20285)
                     /locus_tag="ACHKFK_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HFO9053710.1"
                     /translation="AQDHFGLDVTDIKNKIYNKYRFFRIWFFLQRHKDFAFKPFFTNF
                     NTIERIENYL"
BASE COUNT         4708 a         5185 c         5322 g         5070 t
ORIGIN      
        1 aattgaatga cttttattat tcattatata tatgtgtaga attgtgcgca ggagaaatat
       61 tcactcagga agttattact caggaagcaa agaggattac agaattatct cataacaagt
      121 gttaagggat gttatttccc ggttctctgt ggcataataa acgagtagat gctcattcca
      181 tctcttatgt tcgccttagt gcctcataaa ctccggaatg acgcagagcc gtttacggtg
      241 cttatcgtcc actgacagat gtcgcttatg cctcatcaga caccatggac acaacgttga
      301 gtgaagcacc cacttgttgt catacagacc tgttttaacg cctgctccgt aataagagca
      361 ggcgtttttt tatatatcag aaaggccccg gaggtgcttg cctccgggtg agaaagagct
      421 actgtggcgg gttgttctgc aacgttaaca tcaaaccgtc gcgacgcatc gctgcggctt
      481 cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg taggcgtccg
      541 gacgttgttt cagcgctgcg cggaaggcga gcgatgcttc ctgccattct ccgtgtttca
      601 tcagcgactg acccagtgtg ctccacaaca gcgggcgatc gcccacgttt ttgatctgtt
      661 ggcgcagcac tttttccagc tgttccgggt tattggtttt cagacgcggg atcggcagca
      721 gcaggcgatc gtcatactgg cgtttcaggc cgtcgatgat aatctgctgg gcagtatcat
      781 gatcgtcaca ctcaataaga tgttccgcca ttgccacctg caaggccacc tgatgacgcg
      841 ttttccggct ttggtttttc caccagttac gcaaaccttc actaccgtta tcggcacgcg
      901 cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt tcttcatcac
      961 caacatgggc tttcgccatt gatgggatga tatccagcag cgaactccat gcaccagtgc
     1021 ggatatacgc ctgttccgcc agacgtaata cttccggatg gcgtggcgta acttccagca
     1081 gcttatccac gccgtgacgt gctgcatggt tttcattacg ggccagttgc agacgtacac
     1141 gggtgatttc taccggaatg gtgtcattgc cggccagctc cgctgcgcgt tccagatgtt
     1201 ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggctgccagt agatagttca
     1261 ccaccggttg ttcagcgtga tcggcatttt tcgccattag cttttcaact tgctgataat
     1321 cgccttccgc cagtttcagc agcgcctgtt cggtctgctt acgtgcacgg cgacgcttac
     1381 gtccgacaaa ccacccacgg gtgtgcgcgc ctgtgcggaa gattcgccgc agtagccact
     1441 caatggcaaa cagcactacc atcgccagaa tcaaaataat cgcgaggccc gtgacgctag
     1501 tttcaatatt gtagttgtcg gtctggatca gcacgtaacc ctgatggccg gcaatcatcg
     1561 ggccaaccac gatccccgca atcagcaaca caaagagcaa taacactttt agcatggtta
     1621 ttctccttgc ggcgcggctg ccggagcatc agcttgcggt gcaggtgcag gtgcaggtgc
     1681 aggtgcaggt gcaggtgcag gtgcaggtgc aggtttagct tccgttgtcc ctgctgccgg
     1741 ttgtgccagc aggttacgca cgcgagtctg catcagtttt tccagcatcg cctggctttg
     1801 cagggtttcc gggagatcca tcgagatatt ttgctggctt aactggtcca cctcgtcgag
     1861 gaacgctttg gtggtggcat catcagtatc gtagtaagca cgtacccagg tggagacatt
     1921 ctccagcgcc tggcgataag tctcttcctg atgacgcggt acagcttgtg cagcgaccag
     1981 caggcgagag cgaatatttt cgcgcagata gatatcctga tttggcgcta acagcggtac
     2041 ggcggtgtca tcacgacggc gaatcgtaat gaagttgtcc ataaagttct gccagctttt
     2101 ttgcagattg atacgccatt cgctgatgga actggaaagc tcttcaccgt cggaatccat
     2161 cggcgaacca tcgctgtcat tatcagccag acgcaggtta tctacctgat ttgaaagctg
     2221 attaagctta aggataatgc cgtcataatc cacctgcgat accgcagaaa ggctggcgat
     2281 atcatcggta attgcccggc gaacggtaat cagactcggg tcattcatat ccgccaggct
     2341 ggcgtctgca cttttcagca acgctgcagc ggtcgtgacg tcctgatcgc tccacagctt
     2401 ccgtccggcg agtttcacca gaaaatcggc ctgggccagc agccaggttt tagcatcgct
     2461 gccggaaatg gtggcgacct tttgttggac ttcatccagc tgttttgcca gcgtttcttg
     2521 ctgacgattc gcctgctcaa gctgtacagc ttgttgcttg ataatgcctt ccagctcggc
     2581 tttttggctc tcctgggctt tttgcagtgc cgtaagttgg ttagccaggg catcgctggt
     2641 ggcagtctga ttgacggcct gttgtttacc ccagccatac aaaccgacgc ccgccgccag
     2701 agcaatagcg atggccaccg tgctgagaat caatgcggta ttgttcttac tctttttttc
     2761 tgttgcgaca ggttgtgacg tggtgtccac ggcctccctg gtctcttcaa ccacggcgga
     2821 ggttttttct tgttccgtca ttatggcttc ctgttatgag agttattgta atgcccgtaa
     2881 aagcgcatcg ttgtcagcgt tatcggcgac cttaatgtct tgccagccca gttcccgggc
     2941 gagtttcgcc aaacgctcac tgacgactaa tagccgacag cgtagtaacc agtgctcacg
     3001 ataccattgt gggatcagcg accagagttg ctgcaacatt tcaccgctgg taacaacgac
     3061 cgtcgtcacc ttgcgggatt gccagcgcat cgcttcttct gcaccatcgt aatggattgc
     3121 gcatcgttga taacattcac aaaaagtgac ctcagcaccg cgcgccgtca gggaatcccc
     3181 aattagctca cgaccgccat tgccacgtaa tatcagcgca cgtttgcccg caatattttg
     3241 taattcaggt aattgtagca agacttcgct gatttcccga tcctgcgggt agagaatctt
     3301 ctgtccgctt acggtatgta gtgccagcgc ggtggtgcgt ccaatggcga aataatcagg
     3361 tagtcggggc catttacgat cttgctgatg cagctgtgat tgggcaaaag caaccgcgtg
     3421 ttgcgagagg gcaaacaaca gatcgctctc tcccagcgct gccagttgat cagcaagttg
     3481 cggtaattgt cgacccggag aaaactcaat cagcggaaaa tgccaggcca cctgccccag
     3541 tgtgcgcaga cggctcacta actcttctcc tgcgggagac gggcgggtga ccaggatact
     3601 catgccgggg cgtctccgtt atagacttca gcgaggatct cgcgcgcgcc gttattcagt
     3661 agctcttctg ccagcgaaat ccccatttgt tcggcatctt gcggcgcacc gcggcgttca
     3721 ccgcgaataa tctgcgaacc gtccggcgcg ccgaccaacg cacgcagcca gatttcgcca
     3781 tcaataagct cggcgtagct accaattggc acctgacatc cgccttcgag acgggtattc
     3841 atggcgcgtt cggcggtaac gcgcagtgca gtttcgtggt gattcagcgc ggcgagcagc
     3901 tcgcgagtgc gtgcatcatc aaggcggcat tcaatgccca ccgcaccttg ccctaccgcc
     3961 ggaagagaaa tctcgggtgg caacgcggcg cgaatgcggg actccagacc taaacgtttt
     4021 agtccggcta cggcaagaat gatggcatca tattcgccgt tatccagttt gctcagacga
     4081 gtgccgacgt tgccgcgcag ggagcggata atcagatccg ggcggcgttc agccagttgg
     4141 cactggcgac gtaaactgga cgtcccgacg atactgcctg ccggtaacgc atccagactg
     4201 tcataggtgt tggatacaaa ggcatcgcgg ggatcttcgc gcttgcaaat ggtgaccagc
     4261 cccagacctt gcgggaattc aaccggcaca tctttcattg aatgtacggc gatatcggcg
     4321 cgattttcga ggagcgcgac ttccagctct ttcacaaata agccttttcc gcctactttc
     4381 gccagcggcg tatcaagaat cacatcgccg cgcgtcacca tcggtaccag ttcaacgacc
     4441 aggcccggat ggctcgccat caacttgtct ttgacatagt gtgcctgcca gagtgcaagt
     4501 gggctttggc gtgtggcaat tcttaaaaca ttgtctaaca tgcttgttac cgtcattatc
     4561 atccgtggtc catcctaaca tccttgccag agcgatgtca gtgttgtggt gaaacgtaga
     4621 cgcctgcgca aaccgtaaaa tgaggtctgg cagtggatcc tgacaggcgc tttgagcctt
     4681 cgtaataagg aatttacaga gaataaacgg tgctacactt gtatgtagcg catctttctt
     4741 tacggtcaat cagcaaggtg ttaaattgat cacgttttag accatttttt cgtcgtgaaa
     4801 ctaaaaaaac caggcgcgaa aagtggtaac ggttaccttt gacatacgaa atatcccgaa
     4861 tgccgcgtgt taccgttgat gttggcggaa tcacagtcat gacgggtagc aaatcaggcg
     4921 atacgtcttg tacctctata ttgagactct gaaacagaga ctggatgcca taaatcaatt
     4981 gcgtgtggat cgcgcgcttg ctgctatggg gcctgcattc caacaggtct acagtctact
     5041 gccgacattg ttgcactatc accatccgct aatgccgggt taccttgatg gtaacgttcc
     5101 caaaggcatt tgcctttaca cgcctgatga aactcaacgc cactacctga acgagcttga
     5161 actgtatcgt ggaatgtcag tacaggaccc accgaaaggt gagcttccaa ttactggtgt
     5221 atacaccatg ggcagcacct cgtccgtagg gcaaagttgt tcctctgacc tggatatctg
     5281 ggtctgtcat caatcctggc tcgatagcga agagcgccag ttgctacaac gtaaatgtag
     5341 tctgctggaa agctgggccg cctcgctggg tgtggaagtc agcttcttcc tgattgatga
     5401 aaaccgcttc cgtcataatg aaagcggcag cctggggggc gaagattgtg gctccaccca
     5461 gcatatactg ctgcttgacg aattttatcg taccgccgtg cgtctcgccg gtaagcgtat
     5521 tctgtggaat atggtgccgt gcgacgaaga agagcattac gacgactatg tgatgacgct
     5581 ttacgcgcag ggcgtgctga cgccaaatga atggctggat cttggtggct taagctcgct
     5641 ttctgctgaa gagtactttg gtgccagcct ttggcagctc tacaagagta tcgattcccc
     5701 atacaaagcg gtgctgaaaa cactgctgct ggaagcctat tcctgggaat acccgaaccc
     5761 acgtctgctg gcgaaagata tcaaacagcg tttgcacgac ggcgaaatag tgtcgtttgg
     5821 tctcgacccg tattgcatga tgctggagcg cgtcactgaa tatctgacgg cgatcgaaga
     5881 ttttactcgt ctggatttag ttcgccgatg cttctattta aaagtttgcg aaaaactcag
     5941 ccgtgaacgc gcctgcgtag gctggcgtcg tgaagtgtta agccagttgg tgaaagagtg
     6001 ggaatgggac gattctcgtc tggcaatgct tgataaccgc gctaactgga agatagatca
     6061 ggtgcgtgag gcgcacaacg agttgctcga cgcgatgatg cagagctacc gcaatctgat
     6121 ccgctttgcg cgtcgcaata accttagcgt ttccgccagc ccgcaggata tcggtgttct
     6181 gacacgtaaa ctgtacgccg cgtttgaagc gttaccgggt aaagtgacgc tggtaaaccc
     6241 gcagatttca cccgatctct cggaaccgaa tctgaccttt atttatgtgc cgccgggccg
     6301 ggctaaccgt tcaggttggt atctgtataa ccgcgcgcca aatattgagt cgatcatcag
     6361 ccatcagccg ctggaatata accgttacct gaataaactg gtggcgtggg catggtttaa
     6421 cggcctgctg acctcgcgca cccgcttgta tattaaaggt aacggcattg tcgatttgcc
     6481 taagttgcag gagatggtcg ccgacgtgtc gcaccatttc ccgctgcgct tacctgcacc
     6541 gacaccgaag gcgctctaca gcccgtgtga gatccgccat ctggcgatta tcgttaacct
     6601 ggaatatgac ccgacagctg cgttccgcaa tcaggtggtg cattttgatt tccgtaagct
     6661 ggatgtcttc agctttggcg agaatcaaaa ttgcctggta ggtagcgttg acctgctgta
     6721 tcgcaactcg tggaacgaag tgcgtacgct gcacttcaac ggcgagcaat cgatgatcga
     6781 agccctgaaa actattctcg gcaaaatgca tcaggacgcc gcaccgccag atagcgtgga
     6841 agtcttctgt tatagccagc atctgcgcgg cttaatccgt actcgcgtgc agcaactggt
     6901 ttctgagtgt attgaactgc gtctttccag cacccgccag gaaaccgggc gcttcaaggc
     6961 gctgcgcgtc tccggtcaaa cctggggctt gttcttcgaa cggctgaatg tctcggtaca
     7021 gaaactggaa aacgccatcg agttttatgg cgctatttcg cataacaaac tgcacggcct
     7081 gtcagtgcag gttgaaacca atcacgtcaa attaccggcg gtggtggacg gctttgccag
     7141 cgaagggatc atccagtttt tcttcgaaga aacgcaagac gagaatggct ttaatatcta
     7201 cattctcgac gaaagcaacc gggttgaggt gtatcaccac tgcgaaggca gcaaagagga
     7261 actggtgcgt gacgtcagtc gcttctactc gtcttcgcac gaccgtttca cctacggctc
     7321 aagcttcatc aacttcaacc tgccgcagtt ctatcagatt gtgaaggttg atggtcgtga
     7381 acaggtgatt ccgtttcgca ccaaatctat cggtaacatg ccacctgcca atcaggagca
     7441 tgacgcgccg ctgttgcagc agtatttttc gtgatgaacg tgccggaaag cgaggcttat
     7501 ccggcatgca atcttagcgg aaactaactg tttcacccgc ctgctgcgtc gccgcctgtt
     7561 ccagcaaatc ccagaaggtt tcaccgctac gatcgcaaat ccactcgtcg cctttcagat
     7621 caaaatggta gccaccctgt ttggttgcca gccacacctg gtgcagtggt tcctggcggt
     7681 tgataatgat tttgctgccg ttctcaaagg taatggttag tacaccgccg ttgatttcac
     7741 agtcgatatc gctgtcgcca tcccagtcgt ccagatgttc ttcaatggtc agccacagtt
     7801 gatcagccag gcgatgaaat tcactgtcgt tcattgttgt atcctgtttt taagtgatgg
     7861 cggcagtata gcggcatggg gtcagggctt caaagtttgc ccgtctgctg ctgcgttccg
     7921 gcatgattca tccgtcaccg gaataataat gtcgctgtta tgaatttcac ggacattctc
     7981 ttctctgacg cgtgttttcg ccgggcagtg ggagggcgta atccttacct ctgtatcagt
     8041 ccaggtataa acattgcgat cgtcataagc gaagcgatcg tttttagtta gcggaccgtt
     8101 aactggaata atttgcacgc tcttgcgtga ccgtaattgg cggataaagt aagcatgttg
     8161 tttgccgcta ttaaatgagt catcaatgac caggtctggc gtatccagcg ggattatctc
     8221 aagctggtct tcaccaaaag tcgataattt tgttacatac agtcgatctt tatactgcgc
     8281 aatattgtca gtaatcagat gaaattgttc aggttcaata cccggtaggt ctatccactt
     8341 gctccactga cggtcgggcg tgagctgttt acgccagcga acgcgacttt cccctacttc
     8401 aaaagagacg tcatcatcta cgggtatcgc tttgccagat aattgaccaa agggataacg
     8461 atgcagaccc ttattgtcgc gaaaaaccag cagtgaatga ggtatccagc gaattatctc
     8521 aaatgagtct gcattcgccg taaggtgagc gccatttaaa taaatattct gcccactgcg
     8581 gataagcaga tcattggaat acaaatttct gtttacactc ataagacgtg gattaatacc
     8641 ccaccatctt ttttctaata cagtaacatt actgctgctc ccctgacgtc gaccggacaa
     8701 gtagagattt ttactatcca ccagcgtggt agagttacct tcgatagctt ttagtgtcgc
     8761 cagatcaaca cgtgacgcgc cgctattact ttcggtacgc tgtccgtcaa aatagaggct
     8821 atatttatcg accgcgaagc ggcccatagc ctgaaagctg gcaatatcga cagtgggtgt
     8881 tcccggtgga ttgctaacga ttttgccacg ccagagaata acgtggccgt ctgtcaccca
     8941 gtgatattcc tcattttcta tgcttatttc atcggaaggt atataacccg tcttcccaga
     9001 gatatttttc acaaggcgca aatcggccct gttcatctcc ggaaattggg caatgatttt
     9061 cttatccccc caataacctt taccgtaaga catgacaccg tgatcattgt aatagtcgaa
     9121 ccctatggcc tgtacaggag gtgtcagaaa tgtgttcccc aacaggatga gtgcaactgt
     9181 gattatttga ccattcattt tgttgcctca ttgcgtgggg caggaataaa ttcgccgtca
     9241 tcggtaattt ttccgtccag atatcccgtt ttgtaattta cgccatagcc atatcccgaa
     9301 tgttgttcct tccagattaa gcgaacttgt ttcggatcag cggtgcggta tcgccagagc
     9361 tgcgcgccat caaagctgta aacgtagcgg ttatcgcggg cataccatct gcgcaaaaag
     9421 ggatggtcat taagatgggc ttcgcgttca tccggagagc ggttatccag taagatcagc
     9481 ggtccgtcag attcaaacac gtgtaaactg gaatggttta cccacccttc ggcacgcgta
     9541 agtaaatagc cgtggcgttt cccggcgttg aaacgtttgt tgataataag attggggtca
     9601 tcgagtttta cgatctccaa cttgcggtca ccagactctg tttcgatgat ggtataaagc
     9661 gaatcctgat actgggcgac agcatcactg atggggtgga actgttcagg atcgagtccg
     9721 gataattctt cctgtttaca agcaggccct ttacgccaga acactttctt ttcctgcaga
     9781 gaaaaatcca gacagtgctt tgttaaatct tcatcccacg ccagattttc cttgtcgagg
     9841 actttacgct gcagcccgtt tttatcacgc caggtgaaga gtgagccggg catccagcgc
     9901 acgacggaaa aggtgtccgg gtcggcatcc agttgttctc cgttgatcat gattttcgtc
     9961 tgtgtgcggg ctagcgtatc ccaggggcca aatggggcag gaaggcaggg attaaatgct
    10021 gtagagaatt taccgcgctg atcccatgac ttttgagcca gtaccgtaaa ggtgtcggga
    10081 tcatcaaggc gctgaccatt tgcgtaaaga tagtgcttgt cacggagaat caaacctagc
    10141 cagtctggtt tccattgcgg gttaaattct acctgacgca acgttcggcg gtttagttgc
    10201 tggttgctat cggtgcgttt gccttcgtaa tagaggctgt acttatctgc ggcaaaatca
    10261 ccccatgcct gaaatgacga gatatcgact ggcggtgtgc ctggagggtt ttgcacaatc
    10321 tcgccagcgt agagaatagc gcgaccatcg ctatagtaag agtaatgggg aagagttgcg
    10381 tttgcgccgc tgtagacaac gtctgtcatg agattggtag ccgggggcac cgtttcccaa
    10441 tcctccagtt tggttgggtc aattagcaaa ttcggtaaca gacgcagacg cttgtagtta
    10501 atctgcggta gcgaaagatt ggtcttttca taagggcgca aatacaccac gccttttccc
    10561 tgtttctggt aaatattatg ttccggctca tcaagccgac atccccatgc cgacgataaa
    10621 aacaacgaac aagacaataa acaaaccaca ttgcgatagt gcataaagcc atcctggcgc
    10681 gaggtgccga tcacgaaact accagcaaaa cataaatccc cacgtgtaag cgttatactc
    10741 gcagcatttc ctcacttttc agacttcata aagagtcgct aaacgcttgc ttttacgcct
    10801 tctcctgcga tgatagaaag cagaaagcga tgaactttac aggcaatcca taatgaaaaa
    10861 cgtgtttaag gcactcactg tattacttac tctcttcagc ctgacgggct gcggtctgaa
    10921 aggtccgctc tatttcccgc ctgcagataa aaacgcaccg ccgccgacca aaccggtaga
    10981 gacgcaaacg caatccacgg tgccggataa aaacgacagc gccactggcg atggtccatc
    11041 ccaggtgaat tactaaaagt cagtttctgt acccgcgtga ttggagtaaa tgatgcagtt
    11101 ctcgaaaatg catggccttg gcaacgattt tatggtcgtc gacgcggtaa cgcagaatgt
    11161 ctttttttca ccggagctga ttcgtcgcct ggcggatcgg cacctggggg tagggtttga
    11221 ccaattgctg gtggttgagc cgccgtatga tcctgaactg gattttcact atcgcatttt
    11281 caatgctgat ggcagtgaag tggcgcagtg cggcaacggc gcgcgctgct ttgcccgttt
    11341 tgtgcgcctg aaagggctga ccaataagcg cgatatccgc gtcagcaccg ccaacgggcg
    11401 gatggttctg accgtcaccg atgatgatct ggtccgcgta aatatgggcg aacccaactt
    11461 cgaaccttcc gccgtgccgt ttcgcgctaa caaagcggaa aagacctata ttatgcgcgc
    11521 cgccgagcag acaatcttat gcggcgtggt gtcgatggga aatccgcatt gcgtgattca
    11581 ggtcgatgat gtcgataccg cggcggtaga aacgcttggt cctgttctgg aaagccacga
    11641 gcgttttccg gagcgcgcca atatcggttt tatgcaagtg gttaagcgcg agcatattcg
    11701 tttacgcgtt tatgagcgtg gggcaggaga aacccaggcc tgcggcagcg gcgcgtgtgc
    11761 ggcagtggcg gtagggattc agcaaggttt gctggccgaa gaagtacgcg tggaactccc
    11821 tggcggtcgt cttgatatcg cctggaaagg tccgggtcac ccgttatata tgactggccc
    11881 ggcggtacat gtctacgacg gatttattca tctatgaagc aaccagggga agaactgcag
    11941 gaaacactca cggagcttga tgaccgggcg gttgtcgatt atctgattaa aaatcctgag
    12001 ttttttatcc gcaatgcgcg cgcagtagaa gcgatacgtg tgccgcatcc ggtacgcggc
    12061 accgtttcgt tggtcgagtg gcacatggcc cgcgcacgta atcatattca tgttctggaa
    12121 gagaacatgg cgttgttgat ggaacaggct atcgccaacg aaggcctgtt ttatcgtcta
    12181 ctctacctgc agcgcagtct caccgccgcc agcagtctcg atgatatgct gatgcgcttt
    12241 caccgctggg cgcgcgatct cggcttggca ggtgcgagtc tgcgcctgtt tccagatcgc
    12301 tggcgcttag gtgcgccgtc gaaccacact catctggcat taagccgtca gtctttcgaa
    12361 ccgctgcgta ttcagcgtct ggggcaggaa cagcactatc tgggaccgct taacggaccg
    12421 gagctgctgg tggtgctacc ggaagcgaaa gcggtgggat cggtggcgat gtcgatgctg
    12481 ggaagcgatg ctgatttggg ggttgtgctg tttaccagtc gcgatgccag tcactatcaa
    12541 caagggcagg gaacgcagtt acttcatgaa attgcgctga tgttgccgga gcttctggcg
    12601 cgttggattg aacgcgtatg accgatttac acaccgatgt agaacgctac ctacgttatc
    12661 tgagcgtgga gcgccagctt agcccgataa ccctgcttaa ctaccagcga cagcttgagg
    12721 cgatcatcca ttttgccagc gaaaacggcc tgcaaagctg gcagcaatgt gatgtgacga
    12781 tggtgcgtaa ttttgctgta cgcagtcgcc gtaaagggct gggagctgca agtctggcgt
    12841 tacggctttc tgcgctacgt agcttttttg actggctggt cagccagaat gaactcaaag
    12901 ctaacccggc gaaaggtgtt tcggcaccga aagcgccgcg tcatctgccg aaaaacatcg
    12961 acgtcgatga tatgagtcgg ctgctggata ttgatatcaa cgatcccctc gccgtgcgcg
    13021 atcgggcaat gctggaagtg atgtacggcg cgggtctgcg tctttccgag ctggtggggc
    13081 tcgatatcaa acacctcgac ctggagtctg gcgaagtgtg ggtgatggga aaaggcagca
    13141 aagagcgccg cctgccgatt ggtcgcaacg ccgtggcgtg gattgagcac tggcttgatt
    13201 tgcgcgacct gtttggtagt gaagacgacg cgcttttcct gtcgaaactg ggcaaacgca
    13261 tctccgcgcg taatgtgcag aaacgctttg ccgaatgggg cataaaacaa gggctgaata
    13321 atcacgttca cccgcataaa ttacgtcact cgttcgctac ccatatgctg gagtcgagcg
    13381 gcgatcttcg tggtgtgcag gagctgctgg gtcatgccaa cctctccacc acgcaaatct
    13441 atactcatct tgattttcaa caccttgcct cggtgtacga tgcggcgcat ccacgcgcca
    13501 aacgggggaa ataatgcgtt tttaccggcc tttggggcgc atctcggcgc tcacctttga
    13561 cctggatgat accctttacg ataaccgtcc ggtgattttg cgcaccgagc gagaggcgct
    13621 tacctttgtg caaaattatc atccggcgct gcgcagcttc cagaatgaag atctgcaacg
    13681 cctgcgccag gcggtacggg aagcggaacc tgagatttat cacgacgtga cgcgctggcg
    13741 ttttcgttcg attgaacaag cgatgctcga cgccgggctg agtgccgaag aagccagtgc
    13801 aggcgcacat gcagcaatga tcaactttgc caaatggcgc agccgggtcg acgtcccgca
    13861 gcaaacccac gacaccttaa aacagctggc gaagaaatgg ccgctggtgg cgatcaccaa
    13921 cggtaacgcc cagccggaac tgtttggttt gggggattat tttgagtttg tgctgcgcgc
    13981 tggcccgcac gggcgctcaa aaccgttcag cgatatgtac ttcctggcgg cggaaaaact
    14041 caacgtgccg atcggcgaga tcttacatgt cggggacgat ctcaccactg acgtgggtgg
    14101 ggcaattcgc agcggaatgc aggcctgttg gatcagaccg gaaaatggcg atctgatgca
    14161 aacctgggac agccgtttac tgccgcatct ggaaatttcc cggttggcat ctctgacctc
    14221 gctgatataa tcagcaaatc tgtatatata cccagctttt tggcggaggg cgttgcgctt
    14281 ctccgcccaa cctattttta cgcggcggtg ccaatggacg tttcttacct gctcgacagc
    14341 cttaatgaca aacagcgcga agcggtggcc gcgccacgca gcaaccttct ggtgctggcg
    14401 ggcgcgggca gtggtaagac gcgcgtactg gtgcatcgta tcgcctggtt gatgagcgtg
    14461 gaaaactgct cgccgtattc gattatggcg gtgacgttta ccaacaaagc ggcggcggag
    14521 atgcgtcatc gtatcgggca actgatgggc actagccagg gcggtatgtg ggtcggcacc
    14581 ttccacgggt tggcgcaccg cctgctgcgt gcgcaccata tggacgccaa cctgccgcag
    14641 gatttccaga tcctcgacag tgaagaccag ctacgtctgc ttaagcgttt gatcaaggcg
    14701 atgaatctcg acgagaagca gtggccgccg cgtcaggcaa tgtggtacat caacagccag
    14761 aaagatgagg ggctgcgtcc gcatcatatt cagagctacg gtaatccggt ggagcagacc
    14821 tggcagaagg tgtatcaggc gtatcaggaa gcgtgcgacc gtgcgggcct agtggatttc
    14881 gccgagctgt tgctgcgcgc tcacgagttg tggcttaaca agccgcatat cctgcaacac
    14941 taccgcgaac gttttaccaa tatcctggtg gatgaattcc aggacactaa caacattcag
    15001 tacgcgtgga tccgcctgct ggcgggtgac accggcaaag tgatgattgt tggcgatgac
    15061 gaccagtcaa tctacggctg gcgcggggcg caggtcgaga atattcagcg tttcctcaat
    15121 gattttcccg gtgctgaaac tatccgtctg gagcagaact accgctctac cagcaatatt
    15181 ctgagcgccg ctaacgccct gattgaaaac aataacgggc gtctgggtaa aaaactgtgg
    15241 accgatggcg cggacggtga gcctatttcc ctctattgcg cttttaacga actcgacgaa
    15301 gcgcgttttg tggttaaccg catcaaaacc tggcaggaca acggcggggc gcttgccgag
    15361 tgcgccattc tctaccgcag caacgcccag tcgcgtgtgc tggaagaggc gttattgcag
    15421 gcgagtatgc cgtatcgtat ttacggcggg atgcgcttct tcgaacgcca ggaaatcaaa
    15481 gatgcgctct cgtatctgcg cctgattgcc aaccgcaacg acgacgcggc ctttgagcgt
    15541 gtggtgaata cgccaacgcg gggtattggt gaccggacgc tggacgtggt acgtcagaca
    15601 tcgcgcgatc gccagttaac actctggcag gcatgtcgtg aactgttgca ggaaaaagcc
    15661 ctcgccggac gtgctgccag cgccttacag cggtttatgg agctgatcga cgccttagcg
    15721 caggaaactg ccgatatgcc gctgcatgta cagactgacc gggtaattaa agactccggc
    15781 ctgcgcacca tgtacgagca ggagaagggc gaaaaaggtc agacgcgtat cgaaaactta
    15841 gaggaactgg tgacggcaac gcgccagttc agctacaacg aagaagacga agatttaatg
    15901 ccgctgcagg cattcctctc ccatgcggcg ctggaagcgg gcgaggggca ggcggatacc
    15961 tggcaggacg cggtgcagtt gatgacgcta cactcggcga aaggtctgga attcccgcag
    16021 gtgtttatcg ttggtatgga agagggcatg ttcccaagcc agatgtcgct ggatgaaggc
    16081 gggcgtctgg aagaagaacg ccgtctggcc tacgttggcg taacccgcgc gatgcagaaa
    16141 ctgacgctga cctacgcgga aacccgccgt ctgtatggca aagaggttta ccatcgcccg
    16201 tcgcgcttta tcggtgagtt gccggaagag tgtgtggaag aggtgcgcct gcgcgccacg
    16261 gtaagccgcc cggtcagcca tcaacggatg gggacgccga tggtcgagaa cgacagcggc
    16321 tacaagctcg gccagcgcgt acgccacgct aagtttggtg aaggcaccat cgtcaatatg
    16381 gaaggcagcg gtgagcatag ccgtttgcag gtggcgttcc agggccaggg tattaaatgg
    16441 ctggtggcgg cttacgcccg gttggagacg gtgtgacgtt gcggaatgcg gtgcgccata
    16501 ccgcattccg catcagcacc ttacttagcg cacttcttca catcaatatt ccagtcagca
    16561 ccagacgcaa cctgatgttt ctgcgcgaaa ttatccatat tcagcgccac ggaaacattc
    16621 aataagctgt tcagatacac catcggctgg ccttccggca catcgccaaa gcttgcgaca
    16681 tacggcattt tccctgcgta tttttgctgg gaaccttcgg aaatggtcac acacaacgtg
    16741 tcgttgagtt tgattcctgc ctgattcagc aactcgtcgc tgatattgct ccagacgttg
    16801 ccgtactgaa tatcgagaat cgggatattg cccttcactt caccttttgt tgctttggct
    16861 ttctgataag aaagttcgac aacttttgca ggaagctctg gccccacctg ctcgaacgtg
    16921 atcgccccgg aagccagacg tgcaccggtg taggcataca cgtcacgacc gtggaaggta
    16981 tatgactttt cggaaccttt cagacggttg gttttttcat cgatttgccg gacgctctca
    17041 atacccagcg attccgccac caacgtaagc gtaccgttat ctggtgaaac gaagtactga
    17101 ccgtttttgg ttttcagcac taccgattta cgctctgtac caacacctgg atcgaccacg
    17161 ctaacaaaca ccgaaccttg cggccagtaa ctggcagtct gatacaggcg gtaagcgcct
    17221 tcccagatgt tatacggtgg aatttcgtgt gttaaatcaa atattttaag attatggtca
    17281 acgccgaaag cgaccccttt catcgccgag acggcgccat ctttcaggct aaaatccgtt
    17341 tgtaaaatca aagcgttatc cgcataagcg gatgatgacc ccagacaggt taataaaaat
    17401 aatgttgcga gcgttttttt catttttgtt tcccttcatt tatctgatga ttttccagct
    17461 gtgtagccag ccccagtttg tctggcctcc tcgagtgtac aatgtttaac ctttctgctc
    17521 gacggaaatc atggtgacaa aaggatagcg ttgccacggg attgcctcgc ctttcatata
    17581 catatgtgaa attgtgccat caagataaag cagctgctca acgttcagtt tcgctttggc
    17641 ataacaggca aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcgttccc
    17701 atgtttatta atcccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg
    17761 cggattaatt acaccgtttt ccatcaacaa tggccctgac tgcaccgcaa actgaatctc
    17821 tttactggtt ttaaaggcat ccagacgaac gatgccgact ttatctcccg cgacataaaa
    17881 cactccgcca ggacgaataa agaaattccc ttcacctgaa gcgagattta acgccacctt
    17941 ctgctgaccg ttttcgatgt acaaaccgag cggcgcatag ctttcatcat agatgccgcc
    18001 gttcatcgcc atctgcacct gaccctgact attaatatcc gccagcagag catgtaacgt
    18061 tccccacgct tcgccattgg ctttttgcca gtacattttc acccgctctg tttgaggatt
    18121 aacggtatac gcctgtacgg tcagcgtcgg atctgagggt gcgcaatcat cagcggcaac
    18181 agcaaacaag ggaagtaagg tgagggtgag aaaaatccgt ttgagattca aggtgatcat
    18241 tcctttacca atgaatagcc gatgcgccat tataggccct gaatgtggga tttttttatc
    18301 ctgttagcga ccttgacgag taccaaaaag cgcgaagttc aactattgtt ctgtggtgtt
    18361 ctgttgcgtg ttgacggcaa aattttgctg gcgtaacatg cgcgcacgat cactctaaga
    18421 ggacattcgc cttggacaca cccagtagat actggctcat tatcctgtca tccaggatca
    18481 actcctaagg ctatcccttt ttgctgatag ccttagcggt tgtcagcgac ctcaattttt
    18541 cccgtcgcgc tgagtcaggc tgtttaatgg tctgaaaccc aatttgtttc tgtgtgccca
    18601 ccgaactgtc cgatatttta agcattggga gtcccggtca tgctgagcgc atttcaactg
    18661 gaaaataacc gactgacccg gctggaagtc gaagagtcac aaccccttgt aaatgcagta
    18721 tggattgatc ttgtcgaacc ggacgacgac gagcgactgc gcgtacaatc tgaacttggc
    18781 cagagcctgg caacccgccc ggaactggaa gacatcgaag catcggcccg tttctttgaa
    18841 gacgatgacg gcctgcatat tcactccttc ttcttctttg aagatgcgga agatcacgcc
    18901 ggtaactcca ctgtggcatt taccatccgt gatggtcgtc tgtttactct gcgtgagcgt
    18961 gaactgcccg cttttcgtct gtatcgtatg cgtgcccgta gccagtcaat ggtggacggt
    19021 aacgcctacg agctgctgct ggatctgttc gaaaccaaaa tcgaacagtt ggcagatgaa
    19081 attgaaaata tctacagcga cctggagcag ttgagccggg taattatgga agggcatcag
    19141 ggcgatgagt acgacgaggc gctctccacg ctggcggaac tggaagatat cggctggaaa
    19201 gttcgcctgt gtctgatgga tacccagcgc gcgctcaact tcctggtacg taaagcacgc
    19261 ttaccgggtg gacaactgga gcaggcgcgt gaaatcctgc gagatatcga atccctgctg
    19321 ccgcataacg aatccctgtt ccagaaggtg aacttcctga tgcaggcggc gatgggcttt
    19381 atcaatatcg agcagaaccg catcatcaaa atcttctcgg tggtatccgt ggtgttcctg
    19441 ccaccgacgc tggtcgcttc cagctacggg atgaactttg agtttatgcc ggaactgaag
    19501 tggagcttcg gctacccagg cgcgattatc tttatgatcc tcgcgggcct ggcaccgtat
    19561 ctgtacttta agcggaagaa ctggttgtaa tataaatgcc gggtaaagat tgttctttat
    19621 ccggcttatt gctgaaaatt gtctttttca aagtcatgcg ggttgaacac tcgctctatc
    19681 ttattttcct gggttagttg atactcattg ttatcagcaa taaaaaaatg agtattacgg
    19741 taaggatatt cattaataac cattaaatag ttttttacga tgcaattatc attgctctta
    19801 atcgtcggga aacagtagag atcttcatac ggcccttcga tatatttttt atagtcagtg
    19861 aagccattac cgatatcaat aacataccag gtatgccaac tatcttctgg taggggattt
    19921 tgacttttaa tttcatcctg atgcttcaga taccaattta ttttatcgct atcagtccat
    19981 ggcagatgat caacgaccag aaagactgaa ttgccagcat cagagtatgt atacaaaatc
    20041 tgtacaggac gcaatgtcca taaattatag atgcagcata gaagaaataa atataatata
    20101 attttctgga gttttctttt catagataat tttcgattct ttcgatagtg ttaaaattgg
    20161 tgaaaaaagg tttaaaagca aagtctttat gccgttgtaa aaagaaccat atgcgaaaaa
    20221 atctatactt gttgtaaatc ttgtttttta tatcagtcac atccaaacca aagtggtctt
    20281 gtgct
//
LOCUS       DAZGJT010000085        20278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZGJT010000085 DAZGJT010000000
VERSION     DAZGJT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.085"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1172
                     /locus_tag="ACHKFK_003445"
     CDS             <1..1172
                     /locus_tag="ACHKFK_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO9053711.1"
                     /translation="NFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSI
                     MIAFLIARNVAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVA
                     VIVALITSEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTL
                     ISPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFS
                     EANLENLSWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYR
                     DLAKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVA
                     YAVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATIIYLPFVIAANKVNNMTTNG"
     gene            1200..1511
                     /locus_tag="ACHKFK_003446"
     CDS             1200..1511
                     /locus_tag="ACHKFK_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO9053712.1"
                     /translation="MFADEELVMELLINAGQARSDAMEAIRCAGQKDWQGATKLMASS
                     ESACLQAHKIQTALISQDEGCGKIEVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1566..3239
                     /locus_tag="ACHKFK_003447"
     CDS             1566..3239
                     /locus_tag="ACHKFK_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO9053713.1"
                     /translation="MNIKTLNVSLLSISIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKFRLSTLQKSITENKYQSASLAEISATSPVADNIKNENGEQNS
                     SAAAHTINGSQQIAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGRTANAVVTYDGNVGQQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAREAEFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFTRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDDSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3263..4702
                     /locus_tag="ACHKFK_003448"
     CDS             3263..4702
                     /locus_tag="ACHKFK_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO9053714.1"
                     /translation="MSGFKEDFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAGMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIHFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDLNEREQIIYQAVHYEL
                     VASALAVQTGKLINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPDDNAILARGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFSSHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4759..4977)
                     /gene="marB"
                     /locus_tag="ACHKFK_003449"
     CDS             complement(4759..4977)
                     /gene="marB"
                     /locus_tag="ACHKFK_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO9053715.1"
                     /translation="MKPLLSAIATALILFSAQGVAEQTTQPVVTSCGNVVVVPTSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(5009..5392)
                     /gene="marA"
                     /locus_tag="ACHKFK_003450"
     CDS             complement(5009..5392)
                     /gene="marA"
                     /locus_tag="ACHKFK_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO9053716.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNN"
     gene            complement(5413..5847)
                     /gene="marR"
                     /locus_tag="ACHKFK_003451"
     CDS             complement(5413..5847)
                     /gene="marR"
                     /locus_tag="ACHKFK_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO9053717.1"
                     /translation="MKNTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6059..6724
                     /gene="marC"
                     /locus_tag="ACHKFK_003452"
     CDS             6059..6724
                     /gene="marC"
                     /locus_tag="ACHKFK_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO9053718.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMSSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6749..7939)
                     /gene="ydeA"
                     /locus_tag="ACHKFK_003453"
     CDS             complement(6749..7939)
                     /gene="ydeA"
                     /locus_tag="ACHKFK_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO9053719.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAHSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGMLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWSVTLE
                     EQTQ"
     gene            complement(8081..8200)
                     /gene="yneK"
                     /locus_tag="ACHKFK_003454"
     CDS             complement(8081..8200)
                     /gene="yneK"
                     /locus_tag="ACHKFK_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HFO9053720.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEEQAPFLIVIKM"
     gene            complement(8278..9159)
                     /locus_tag="ACHKFK_003455"
     CDS             complement(8278..9159)
                     /locus_tag="ACHKFK_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO9053721.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILTLVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNRRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVTRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDAPDSAKQGYQ"
     gene            9260..10648
                     /gene="sad"
                     /locus_tag="ACHKFK_003456"
     CDS             9260..10648
                     /gene="sad"
                     /locus_tag="ACHKFK_003456"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO9053722.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGVDDIENALQLAAAGFRD
                     WREANIDYRAEKLRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSLMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITIAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRV"
     gene            10712..11638
                     /gene="glsB"
                     /locus_tag="ACHKFK_003457"
     CDS             10712..11638
                     /gene="glsB"
                     /locus_tag="ACHKFK_003457"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO9053723.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            11638..11997
                     /locus_tag="ACHKFK_003458"
     CDS             11638..11997
                     /locus_tag="ACHKFK_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO9053724.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGVKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGEALSEEQQR
                     YIVTVIYHWLVIQMNQP"
     gene            12136..13554
                     /locus_tag="ACHKFK_003459"
     CDS             12136..13554
                     /locus_tag="ACHKFK_003459"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO9053725.1"
                     /translation="MHVQPISTFRLFQEGHLLRNSIAIFVLTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLAINFSNMMFI
                     VTVALLVARDKRLGKNKYEPVSALRLFNYCLLAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEVVLVANSVIDISVGSPFSIPQMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFAQHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGCASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            13781..15232
                     /gene="uxaB"
                     /locus_tag="ACHKFK_003460"
     CDS             13781..15232
                     /gene="uxaB"
                     /locus_tag="ACHKFK_003460"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO9053726.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQYRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            15429..16343
                     /locus_tag="ACHKFK_003461"
     CDS             15429..16343
                     /locus_tag="ACHKFK_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO9053727.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANHIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(16347..17105)
                     /gene="tam"
                     /locus_tag="ACHKFK_003462"
     CDS             complement(16347..17105)
                     /gene="tam"
                     /locus_tag="ACHKFK_003462"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO9053728.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYIADLGCGPGN
                     STALLDQRWPAARITGIDSSPAMIAEARSALPDCLFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLSPLGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQHFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            17413..17679
                     /gene="hipB"
                     /locus_tag="ACHKFK_003463"
     CDS             17413..17679
                     /gene="hipB"
                     /locus_tag="ACHKFK_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO9053729.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEAAEQQDLEW"
     gene            17679..18693
                     /locus_tag="ACHKFK_003464"
                     /pseudo
     CDS             17679..18693
                     /locus_tag="ACHKFK_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
     gene            complement(19068..19529)
                     /locus_tag="ACHKFK_003465"
     CDS             complement(19068..19529)
                     /locus_tag="ACHKFK_003465"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9053730.1"
                     /translation="MAVDKECAERIRLSIIQNFTYDDIENETGISVSTLKRLASGERE
                     PKLIEIRKIAKATGKNPVWLAFGESTLPLPENLGEDMTAFGYGIDFKLQGHETGEAAA
                     KIRLMAAIRDIEKDDIVFLEKFIDLLNLDRTINNVKTKRYGHTEEEARFKK"
     gene            19732..20202
                     /locus_tag="ACHKFK_003466"
     CDS             19732..20202
                     /locus_tag="ACHKFK_003466"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053731.1"
                     /translation="MTESAILTAYNAIDAASVALGRPCLTLPEREHFRTERDVATRNL
                     ATLSRQYRDEVGTLSAQLALPRNTQVDRVIYGINCTDLSLADRGRAAGLVSQFGSFSF
                     TVAHAFCRLVYQLGVKNSLMALESAHRFAFFNQDGDPYEFSLFCTDEQIAVNDG"
BASE COUNT         5162 a         4639 c         5021 g         5456 t
ORIGIN      
        1 ttaactttgc catattatcg cccgacggtt ttattgccgg attactgcat ctgaacagca
       61 ttttccccaa ccttgaaaaa gcacaagcta tttttactcc ggtaatgaat ggttctgtaa
      121 atatcatgtc aattatgatt gctttcctga tcgccaggaa tgtggcgatt agctatgagc
      181 aagatgatct tttatgcgga ttaacggcaa taggagcatt ttttattgtt tataccccat
      241 accagatgat agatgggcag gcattcctga cgaccaaata tctcggcgcg caggggttgt
      301 ttgttgctgt tatcgttgca ttgatcacca gtgaaatatt ttgtcgctta gctcgaaacc
      361 ccaaaatcac cataacgatg ccggcagctg tacctcctgc ggtagcgcgt tcatttaaag
      421 ttttattgcc aatatttttt gttatggtgt tcttttccgc acttaattat tgcctgacat
      481 tgatatcccc ggcaggatta aatgatctca tttacacatt aatccagacg ccgctcaaac
      541 atatgggaac gaatatcttt gcggtaatta tcctgggggc tgtgggtaat ttcctctggg
      601 tgctggggat ccacggacct aatacaacgt cggcaattcg agaaaccgtt ttttctgagg
      661 ctaatctgga gaatctctcc tgggccgctc aacacggcac tacctggggc gcgccatatc
      721 cgattacctg gacttctatt aatgatgcat tcgccaactg cggcggttca ggtatgacgt
      781 tggggttatt gttggctatt tttatcgctt ctaagcgtgc ggaataccgt gatctggcaa
      841 aaatgtcatt tatccccggt attttcaata tcaatgaacc gataatgttc ggccttccta
      901 ttgtacttaa ccccatcatg atggtgccgt ttattatggt tcccattgtt aactgtgcca
      961 ttggttactt ctttgtttcg atggaaatta ttccaccggt tgcttatgcc gtgccctgga
     1021 ctacgcccgg acctttaatt gctttcctcg gaaccggggg aaactggctg gcattactgg
     1081 ttggtttttt atgtttaggt gtggcgacaa tcatctattt accttttgtt atcgccgcca
     1141 acaaagtcaa taacatgaca actaacggat aattccattt acccaggaag gagattgtga
     1201 tgtttgcaga tgaagaactt gttatggagt tactgatcaa cgcgggacag gctcgttctg
     1261 atgcgatgga agccatacgt tgtgccgggc aaaaagactg gcagggcgca actaaactaa
     1321 tggccagctc tgaatctgcc tgtcttcagg cgcataagat tcagaccgcg ttaattagtc
     1381 aggatgaagg ctgtggcaaa atagaagtta atttaattct gatacatgca caggatcatt
     1441 tgatgaatgc aatattatgc caggatttag ccagggaaat tatttcatta agaaaagagc
     1501 tccatgcctg atttttcctc cagtgcgata tctggaggaa attactacac tcttaaggat
     1561 aagtcatgaa tataaagacg cttaacgtca gccttttgtc tatttctatt attacagcat
     1621 tgtttccgtt gaacgcgatg gcaacaaaat taaccataga gcagcgcctt gaattgcttg
     1681 aaaatgaatt gtcgcaaaat aaacaagagc tgaaagcaac acagaatgaa ctgggagtat
     1741 ataaattccg actttcgaca ttacaaaaaa gcatcacaga aaataaatat caatcggcct
     1801 cgcttgccga aatatcagcg acatctcccg ttgctgataa catcaaaaat gaaaacggtg
     1861 aacagaactc gtctgccgca gcacatacta taaatggatc gcagcaaatt gccgttattg
     1921 aaagtaaagg cgataaaacc actatcgaaa gcgtgaccct gaaagatatc agtaaatata
     1981 taaaggatga tattggattc agctatcagg ggtacttccg ctcaggctgg ggcaccggta
     2041 atcacggctc accacaaact tatgcagcgg gttctctggg acgttttggt aacgagatga
     2101 gtggttggtt tgacctgacc ttaaatcagc gtgtttataa tcaggacggt agaacggcaa
     2161 atgcggtcgt tacctatgat ggaaacgtag gtcagcagta taacgatgcc tggtttggtg
     2221 acagtgccaa tgaaaatatc atgcagttca gtgatattta tctgacaacg cgaggttttt
     2281 tacccttcgc acgagaggca gaattctggg tcggcaaaca taaactcccg caatatgaga
     2341 tccaaatgct ggactggaaa accttaacca cggatgttgc cgcgggtgtg gggattgaaa
     2401 actgggcgct tggtgtaggg ctgtttgata tgtccttaag ccgagatgat gtcgatgttt
     2461 actctcgtga ttttacgcgt accagtcaga tgaataccaa ttctgtggat gttcgttatc
     2521 gcaatatccc gttatgggat gatgcgacgt tgtcattaat ggctaaatat tccgcaccta
     2581 ataaaacgga tcaacaacaa gataatgaaa atgacgacag ttattttgaa atgaaagata
     2641 gctggatgct gacttctgtt ttacggcaaa acttgcaacg cgatacgttt aatgaattta
     2701 cgttacaggt tgccaataat tcctatgcca gcagttttgc cagtttctca gatgccagta
     2761 atacgatggc gcatggtcgc tattactatg gtgaccatac caatgggatc gcctggcgtt
     2821 taatctctca gggcgagatg tatctgactg acaatattat tatggctaac gcgcttgtct
     2881 attctcatgg cgaagatgtt tatagttatg aaagtggcgc tcatagtgat tttgacagta
     2941 ttcgcaccgt aataagaccg gcctggatct ggaatacatg gaatcagacg gggcttgaat
     3001 taggctggtt taagcaacag aacaaaactc agcagggggt aacgctaaat gaatcggctt
     3061 ataaaacgac actctggcat gcattgaaag tgggtgaaag tattttaggt tcacgaccag
     3121 aaattcgctt ctacgggacg tatatcaata ttctggataa cgaattatcg aattttaagt
     3181 ttaatgagaa cagcaaagac gagtttatgg ccggcatcca ggcggaagtc tggtggtaat
     3241 ctatttagtt aaggagttgt gtatgtcagg atttaaagaa gattttttat ggggcggtgc
     3301 ggtagccgca catcaattgg aaggtggctg gaatgaagga ggaaaaggca tcagtatcgc
     3361 tgatgtaatg actgctggcg ctcacggggt gccgcgtgaa gtgacagaag gcgttatcga
     3421 cggacttaat tatcccaatc atgaagcaat tgatttttat catcgctata aaacagatat
     3481 tcagttattt gccgggatgg gattcaaatg ctttcgaact tccattgcct ggacacgaat
     3541 ctttccgcaa ggtgacgaac aggagccgaa tgaagagggt ttacaatttt atgatgatct
     3601 cttcgatgaa tgcctgaagc agggaatgga acctgtggtg acgctttcgc attttgagat
     3661 gccttatcat ctggtgacaa aatatggtgg ctggcgaaac cgtaaactga tcgacttttt
     3721 tatccacttc gcatcaacgg tcttcacgcg ctataaagaa aaagtaaagt actggatgac
     3781 gtttaacgaa atcaataatc aggtgaattt cagcgaaagc ctgtgtccat ttactaattc
     3841 cggtatcttg tattcgccag aggaagatct caatgagcgc gaacaaataa tataccaggc
     3901 ggtacattat gagttagttg ccagtgccct agcggtacag actggaaaac tgatcaatcc
     3961 tgaatttaat atcggctgta tgattgctat gtgccccatc tatcctctga cgtgtgcacc
     4021 caacgatatg atgatggcca cgaaagcgat gcatcgtcgt tactggttta ctgatgttca
     4081 tgctcgtgga tattatccgc aacatatgct gaattacttt gcaaggaaag gattcaacct
     4141 cgatatcaca ccagatgata acgcgattct tgccagaggt tgtgtcgact ttatcggctt
     4201 tagctactac atgtctttta cgacgcaatt ttcgccagat aatccgcaac tggattatgt
     4261 tgaaccacga gatttggtca gcaaccctta tatcgataca tccgaatggg gatggcaaat
     4321 tgatccggca gggctacgtt attcactcaa ctggttctgg gatcatttcc agttgccgct
     4381 gtttattgtc gaaaatggat ttggtgcggt tgaccagaga caagctgacg gcacggtgaa
     4441 cgatcactat cgcattgatt acttttcctc ccatattcgg gaaatgaaaa aagccgtagt
     4501 tgaagatggt gttgacttaa ttggctacac cccgtggggt tgcattgacc tggtttctgc
     4561 cggaacagga gaaatgaaaa aacgctacgg aatgatttat gtcgataaag acaacgaagg
     4621 gaagggaacg ctggaacgga tacgtaaagc atcgttttac tggtatcggg atctcatcgc
     4681 caacaatggc gaaaatatat gagacttcag gaagatatta cggaaatgtc aggctctgtg
     4741 atcagagcct gacaataact acatagcgtg ttgattataa tagggcacgc cgagcgcatc
     4801 cgacttatca ctgccagtac ccatgtgatt taaatcaaac ggtggttgtt cctgcgatgt
     4861 gggaacaacc acgacattgc cacaggaagt aacaaccggc tgcgtggttt gttccgcaac
     4921 gccctgcgca gaaaagagaa taagcgcagt tgctattgcg gataaaagtg gtttcatgat
     4981 tgcctcagtg acgttgtcac gttatcaact agttgttgta atgatttaat ggatgtaaaa
     5041 agcgcgattc accctgcata ttggtcatcc ggtatttatg cggcggaaca tcaaagtaat
     5101 ttttgaaggt tcgggtcaga gtttgttggg actcgaagcc atatcgttcc gccagataga
     5161 gtatcggctc gttactttcc ttcagctttt gcgcgatttc cgtcatctta cggctacgga
     5221 tgtattggcc taatgaatga ccggtttctt ttttaaacat ccgttgcagg tgccatttgg
     5281 agtaacccga acgctctgac actttctcca gtgacagtgg cgattccagg ttgtcctcga
     5341 tccagtccaa aatgctatga atggtaatag cgtcagtatt gcgtctggac atcgtcatac
     5401 ctcttttttc gtttacggca ggactttctt aagcaaatgc tcaagtgttg ccacttcgtc
     5461 cgccgtcagg ttttttgtta attcttgatg caggtcctgg ccaactaatt gatggcattg
     5521 ttcacatatt gccgcgccgc tggtggtaag ttttaccagt acgccgcgct tatcattcgg
     5581 gttcggcaac ctttccaccc agcctttaca gaccaggcga tccagcatac gggtcagtgc
     5641 ccccaggtcg accgacaaca cttttttcag ttcaaccgga gtaatacacg ccgcgcagcg
     5701 gatagagcag agcaccttaa actgtgccgc ggtaatatcc agcggagaca gatactcgtt
     5761 aagcaggcga tctttcttct gattaaccat atggattaag cgacccaatg gaataatttc
     5821 attgaacaga tcgctggtat ttttcacatt agttgccctg gcaagtaatt agttgcaggg
     5881 gataatattg cccaggcaag tataagtcaa atgaatgaat tgaccgatgc cacgttttgc
     5941 taaatcggtt caagttttgg tcaatgcgat gcaaggttag ctaaagagtg acactttatc
     6001 cttgtttacc ccttatactt ttcgctgata aaccagatac acaggataac aaccaccaat
     6061 gttagatttg tttaaagcaa taggcctggg gctggtggtg ttactgccgt tagctaaccc
     6121 attgacaact gtcgcgttat ttcttggcct ggcaggcaac atgagcagtg ccgaacgtaa
     6181 tcgtcagtcg ttgatggcct cggtgtacgt atttgccatc atgatggtgg cgtattacgc
     6241 cgggcaactg gtgatggata catttggcat ttcgattccc ggtctgcgaa ttgcaggcgg
     6301 cttaatcgtc gcgtttatcg gttttcggat gctctttccg caacagaaag cgattgattc
     6361 accggaagcg aaaagcaagt cggaagagct ggaagatgaa cccagtgcca atattgcttt
     6421 tgtaccactg gcaatgccaa gtaccgccgg tccgggaacc attgcgatga ttattagctc
     6481 ggcctccaca gtgcgtcaga gttcaacctt cgctgattgg gtactgatgg ttgcgccgcc
     6541 gctgatcttt ttcctggtgg cggttatttt gtggggaagt ctacgcagct ctggcgcaat
     6601 tatgcggctg gtgggcaagg ggggtattga agcgatctcc cgtctgatgg gattcctgct
     6661 ggtatgtatg ggcgtgcagt ttattattaa cggcatcctg gaaatcatta aaacgtatca
     6721 ttaaaaaagg cccgaatggg cctttcgact attgcgtctg ttcttcgagt gtcactgacc
     6781 agcggcgaaa aatgatgatt gaccatatta acgcagcaaa agcaggcacc gcgcctacat
     6841 aaccaatcat cgacattgac cagtgcagac tcacctgatt acctaccaac gcacccgcgc
     6901 cgattccaat attaaatatc ccggagaata gcgccatcgc gacgtcggtg gcatctggtg
     6961 ccagcgccag cactttaacc tgcataccaa gcccgatgat catcatcgcg atcccccaga
     7021 aaatactcag catcccgagg tgtatttcac tgttcgccgc aggtagcagc agtgccaggc
     7081 acaccagcaa tagcgcaatc gctgtactca ccaacgcaga cgcatactga ttacccagtt
     7141 taccgaaaat cacgctgcca ataatgcccg caccaccgag taataacagt aatgccgtgg
     7201 caaagttggc gctgaatccc gcaatgtttt gtacaaaagg ctcgatatag ctgtatgccg
     7261 tgtaatgggc ggtgacaacc accacagtta acaaataaat gctcatcaat gccgggcggc
     7321 ggaacaatag cgggaggctt ttcaatgaac cggaatgctc actgggcagc aagggaagta
     7381 acttaatcag gcacaaaagg gtaataagcg ccccgatacc aatcgcgaag aaggtcattc
     7441 gccagccaaa atactgcccc acaatgcgcc cgagaggtaa acctaacacc attgccagtg
     7501 ctgtaccggt ggcaattaaa ctcaatgcct gtgctcgctt cccggccgga gccatacgga
     7561 tcgccagaga cgccgtaatc gaccagaaaa tcgcatgtgc aaaagccaca ccaatgcgac
     7621 tgatcaccag aacggtaaaa ctccacgata aaaacgacag tacgtggctg gcaataaaca
     7681 ccacaaacag gcagatcagt aatttgcggc gttcaacctg gctggtcatt aacataaaag
     7741 gcaatgacat tagcgccact acccatgcat aaattgtcaa catgatgccg acctgagcgg
     7801 tttgcatgtg aaaactatgc gcaatgtcag agagcaggcc aacagggaca aattcggtgg
     7861 tattgaagat aaaggcggcg actgccagcg taacgacccg tagccacgct actttgcggg
     7921 aaacagtgtt tgttgtcata acagattatc gggtgagttg cgaaaaagtg atgaaatgat
     7981 cttaaaacgg ttaatgatgt gaatacaatg catttgtgat ctggatcgca tttccatctc
     8041 tctgacggtt gttctgtcac gcatcagaga gttgttaaca tcacatttta atgacgatca
     8101 ggaagggggc ctgttcttct tttacttgag gtgcaatatt acgaacggga aaatcatcag
     8161 gaagagatat gtaattgctg atgcttactg gcgtaaccat atttgcccca tatagtcata
     8221 agtcatcgga acatcactat atagagcata ttagccgcat gaactatcaa aaatagctca
     8281 ttggtaaccc tgttttgccg agtcaggcgc atccagcagt tgaataaatg cctcaagcgg
     8341 acgcgttttc gcaccacgac gccagaccag ccaggttgtt aaccaacgcc attgctcagc
     8401 taacggccac gcttcaacct ggtgatgccc cggcatactt tccagcattg agcgtggaat
     8461 aagcgcaatg cctgctcctg cgaccacaca ggccaacatc ccgtgataag actccatctc
     8521 gtgaatagtt cccggagcgg caccgtcagc atgaaaccag ctttcgaagt ggcgacgata
     8581 cgaacaattg gcgcggaagg cataaatgtt actgccattc acctgactgg cacgggttac
     8641 tggcgcatgt ccttgtggcg tgacgatcat tagttcctcg cggtataccg gtatcccgtc
     8701 gatggcagta tggttaatgg gtccatcaat aaacgccgca ttcagttttc cctccagcac
     8761 accttccagc atagtgccgg aagggccggt ggaaagtgaa aactgaattt tgggataacg
     8821 acggttaaat tccgccagcg tggccggaat gcgcactgca gcggtgcttt ccagcgatcc
     8881 cagagaaaat aaaccttgcg gctcatcgcc agcgacaacg ctccgcgctt catccactaa
     8941 cgtgagaatt tgttggctat agcgtaaaaa gttatgcccg gcaggcgaga gacgtaaacg
     9001 ctgattctcg cgaataaaca gatcaacccc cagttctgtt tccagctggc gtagacgggt
     9061 agtgagattc gacggcacgc gatgcacttt tgctgcagcc tgggtaatgc tgccagcttc
     9121 ggcaaccgcg ttgaacatct ccagttgggt cagatccata gcattctcga ttcgtgaata
     9181 agtggcttaa tattattcat tttaaagcaa gagtaaatct gcgtatcttc ataccatgac
     9241 tcataaagga gataccccga tgaccattac tccggcaact catgcaattt cgataaatcc
     9301 tgccaccggt gaacaacttt ctgtgctgcc gtgggctggt gttgacgata tcgaaaacgc
     9361 gcttcagctg gcagcagcag gctttcgcga ctggcgcgag gcaaatatag attatcgtgc
     9421 tgaaaaactg cgtgatatcg gtaaggctct gcgcgcccgt agcgaagaaa tggcgcaaat
     9481 gatcacccgt gaaatgggca aaccaatcaa ccaggcgcgc gctgaagtgg cgaaatcggc
     9541 gaatttgtgt gactggtatg cagaacatgg tccagcaatg ctgaaggcag aacctacgct
     9601 ggtggaaaat cagcaggcgg ttattgagta tcgaccgttg gggacgattc tggcgattat
     9661 gccgtggaat tttccgttat ggcaggtgat gcgtggcgct gttcccatca ttcttgctgg
     9721 taacggctac ttacttaaac atgcgccgaa tgtgatgggc tgtgcacagc tcattgccca
     9781 ggtgtttaaa gatgcgggta tcccacaagg cgtatatggc tggctgaatg ccgacaacga
     9841 cggtgtcagt ctgatgatta aagactcgcg cattgctgct gtcacagtga ccggaagtgt
     9901 tcgtgctgga gcggctattg gcgcacaggc tggagcggca ctgaaaaaat gcgtactgga
     9961 actgggtggt tcggatccgt ttattgtgct taacgatgcc gatctggaac tggcggtgaa
    10021 agcggcggta gccggacgtt atcagaatac cggacaggtt tgtgcagcgg caaaacgctt
    10081 tatcatcgaa gagggaattg cttcggcatt taccgaacgt tttgtggcag ctgcggcagc
    10141 cttgaaaatg ggcgatcccc gtgatgaaga gaacgctctc ggaccaatgg ctcgttttga
    10201 tttacgtgat gagctgcatc atcaggtgga aaaaacactg gcgcagggtg cgcgtttgtt
    10261 actgggaggg gaaaagatgg ctggagcagg taactactat ccgccaacgg ttctggcgaa
    10321 tgttacccca gaaatgaccg cgtttcggga agaaatgttt ggccccgttg cggcaatcac
    10381 cattgcgaaa gatgcagaac atgcgctgga actggctaat gatagtgagt tcggcctttc
    10441 agcgaccatt ttcaccaccg acgaaacaca ggccagacag atggcggcac gtctggaatg
    10501 cggtggggta tttatcaatg gttattgtgc cagcgatgcg cgagtcgcct ttggtggcgt
    10561 gaagaagagt ggctttggtc gtgagctttc ccatttcggc ttacacgaat tctgtaatat
    10621 tcagacggta tggaaagacc gggtctgacg ctatatattc agctctgata tactcgcagg
    10681 tcttttcaga cctgcggtcc aggagtagaa agtggcagtc gccatggata atgcaatttt
    10741 agaaaacatc ttgcggcaag tgcggccgct cattggtcag ggtaaagtcg cggattatat
    10801 tccggcgctg gctacggtag acggttcccg attggggatt gctatctgta ccgttgacgg
    10861 acagcttttt caggccggag acgcgcaaga acgcttttcc attcagtcta tttccaaagt
    10921 gctgagtctc gttgtcgcca tgcgtcatta ctccgaagag gaaatctggc aacgcgtcgg
    10981 caaagatccg tctggatcac cgttcaattc tttagtgcaa ctggaaatgg agcagggtat
    11041 tccgcgtaat ccgtttatta atgccggtgc gctggtggtc tgcgatatgt tgcaagggcg
    11101 attaagcgct cctcggcaac gtatgctgga agtcgtgcgc ggcttaagtg gtgtgtctga
    11161 tatttcctac gatacggtgg tagcgcgttc cgaatttgaa cattccgcgc gaaatgcggc
    11221 tatcgcctgg ctgatgaagt cgtttggcaa tttccatcat gacgtgacaa ccgttctgca
    11281 aaactacttt cattactgcg ctctgaaaat gagctgtgta gagctggccc ggacgtttgt
    11341 ctttctggcg aatcagggga aagctattca tattgatgaa cctgtggtga cgccaatgca
    11401 ggcgcggcaa attaacgcgc tgatggcgac cagcggtatg taccagaacg cgggggagtt
    11461 tgcctggcgg gtgggactac cggcaaaatc tggcgttggt ggcggtattg tggcgattgt
    11521 tccgcatgaa atggccatcg cagtctggag tccggaactg gatgatgccg gtaactcgct
    11581 tgcgggtatt gccgtccttg aacaattgac gaaacaatta gggcgttcag tttattaatg
    11641 cagtctctcg atccactctt cgcgcgttta tcccgttcaa aatttcgctc tcgctttcgt
    11701 ttgggggtga aagagcgtca gtattgtctg gagaaaggcg cgccagtcat tgaacaacat
    11761 gcggcggatt ttgtcgctaa acgtcttgct ccggcattac cggctaacga cggtaagcaa
    11821 acccccatgc gcgggcatcc ggtttttatc gctcaacacg ccaccgcgac ctgttgtcgc
    11881 ggttgtctgg ctaagtggca taacattcct cagggggaag cgttaagtga ggagcaacaa
    11941 cgttatattg tgacggtaat ctaccactgg ctggttatac agatgaatca gccatgataa
    12001 ttacggaaat cgataaaatg gcattcatta ggtatcttta gttgccgccg ttgtcagcga
    12061 acatgctatc ctttctaaga atttacttat tgcgttgaga taattgccac ccgcaattgt
    12121 atggacagat aattaatgca tgtacagccc atttcaactt tccgtttgtt ccaggaaggt
    12181 catctgctac gtaatagcat cgctattttt gtgctaacca cgctgttcta ttttattggt
    12241 gcagagttac ggctggttca cgaactttct cttttctggc cgctgaatgg cgtaatggcg
    12301 ggggtgtttg cccgctatgt ctggcttaat cgactgcatt actatgcgat cagttatgtg
    12361 gcgatgctgg tttatgatgc cataaccacc gaatgggggc tggtttcact ggctatcaat
    12421 ttctccaata tgatgtttat tgttaccgtc gccttactgg tcgcgcggga taagcgtctg
    12481 ggaaaaaata agtatgagcc agtaagtgcc ttacggctat ttaattactg tctgcttgcc
    12541 gcattattat gcgctattgt cggggcgatt ggttcggtca gtattgatag tctggatttc
    12601 tggcctttgc ttgccgactg gtttagtgag caattctcaa cgggcgtgtt gatcgtgcct
    12661 tgtatgctga cgttggcaat tcctggagtg ctgccgcgct ttaaagcaga gcagatgatg
    12721 cctgctatcg cgcttattgt gtcggttatt gcctcggtag tcattggcgg agcggggagt
    12781 ctggcgtttc cgctccctgc attaatctgg tgtgcagtgc gctatacgcc gcaggtaaca
    12841 tgtctgttga cctttgtcac cggtgcggtg gaagtcgtac tggtggcaaa ttcggtgatt
    12901 gatatctcgg tcggttcgcc gttctccatt ccgcaaatgt tctccgcacg tctcggtatt
    12961 gccacgatgg cgatatgccc aattatggtt tcctttagcg tggcagcgat caattcgcta
    13021 atgaagcaag ttgcgctgcg agccgacttt gattttctga ctcaggttta ctcacggtcc
    13081 ggtctttatg aggcgctgaa aagtccatcg ctgaaacaga cacaacatct gacggtcatg
    13141 ctgcttgata tcgactattt caaaagcatt aacgataact atggacatga atgtggcgat
    13201 aaagtgttaa gcgtgtttgc ccagcatatt cagaagattg tcggtgataa ggggctggtg
    13261 gcgcgaatgg gcggcgaaga atttgctgtt gcagtgccgt cggtgaatcc tgtcgatggc
    13321 ctgctaatgg cggaaaaaat ccgtaaaggc gtagaacttc aaccgttcac ctggcaacaa
    13381 aaaacgcttt atctcacggt aagtattggc gtcggtagtg gctgcgcatc gtaccgaacg
    13441 ctgaccgatg actttaataa attgatggtc gaagccgata cgtgtctgta tcgctcgaag
    13501 aaagatgggc gcaaccgtac cagcactatg cgttacggtg aggaagttgt ctgacatctt
    13561 tatttactca gagcgagtta aatgcaatgt gttctaccac tttatgatcc aagaaaaggc
    13621 ctgtttcctc gattaaacca tgatccgcgc cacacttttc tcatacaact gctcattaat
    13681 catcaggagt tgatcaaaac aggggttcgg cgtattaaag tttgctaacc tgtcgtacca
    13741 attaaaacag gtagtatgac taatcagaag ggaacccatt gtgaaaacac taaatcgtcg
    13801 cgattttccc ggtgcacagt atccagaacg tatcattcag tttggtgaag gtaacttcct
    13861 gcgcgccttt gttgactggc aaatcgatct cctgaatgag cacaccgatc tgaattctgg
    13921 cgtggtcgtt gttcgtccga ttgaaacttc attcccgccg tcactgagca cgcaggatgg
    13981 tctgtatacc accattatcc gtggcctgaa tgagaagggt gaagcggtca gcgacgcgcg
    14041 tctgattcgc tcggttaatc gtgaaatcag cgtctacagt gaatacgatg aattcctgaa
    14101 actggctcac aacccggaaa tgcgctttgt tttctctaac accaccgaag cgggtatcag
    14161 ctaccatgcg ggcgataaat tcgatgacgc gccagcggta agttatccgg caaaactgac
    14221 ccgtctgctg ttcgaacgtt ttagccattt caacggtgcg ctggataaag gttggatcat
    14281 catcccgtgt gagttgattg actataatgg cgacgcgttg cgtgagctgg tgctgcgcta
    14341 tgcgcaagag tgggcactgc cagaagcatt tattcaatgg ctggatcagg ctaacagctt
    14401 ctgttctacg ctggtggacc gtatcgttac cggttatccg cgtgatgaag tggctaaact
    14461 ggaagaagaa ctgggctacc acgatggttt cctcgacacc gctgaacact tttacctgtt
    14521 tgtgattcag gggccgaaat ccttagcgac tgaacttcgt ctggacaaat atccgctcaa
    14581 cgtgctgatc gttgacgata ttaaaccgta taaagaacgc aaagtggcaa tcctcaacgg
    14641 tgcgcacacc gcgctggtgc cggtggcgtt ccaggcaggg ctggataccg taggtgaagc
    14701 gatgaacgat gctgaaattt gcgcattcgt tgaaaaagcc atctacgaag aaattattcc
    14761 ggtactggat ttgccacgcg atgagctgga gtctttcgcc agtgcagtca ccggacgttt
    14821 ccgtaacccg tacatcaagc atcaactgct gtctatcgcg ctcaacggta tgaccaagtt
    14881 ccgcacacgc atcctgccgc agctgctggc agggcagaag gcaaacggca cacttccggc
    14941 gcgccttact ttcgcattag cggcattgat tgcgttctat cgcggtgagc gtaatggaga
    15001 aacatatccg gtacaggacg atgcacactg gctggaacgt tatcagcaac tctggagcca
    15061 gtatcgtgac cgtgttatcg ggacccagga actggtagcg attgtgttgg cagaaaaaga
    15121 ccactgggag caggacctga cgcaagtgcc tggtctggtc gagcaggttg ctaatgacct
    15181 ggatgcgatt ctcgaaaaag ggatgcgcga agctgtacgc ccgttgtgct aatactctga
    15241 acccggtttc gaccgggttt cttatttcac tatattagtt acaacatcct atgcgtcttt
    15301 atcattaaca ggtgccacat gcagcacctg ttaatgttgt aagcttgtag gtaatttgta
    15361 agtagtgtct tgagaatgtg atcgtaatca cgtttaatag ggcgactttt ccacgcctga
    15421 agataaccat gattgttcgt ccacaacaac actggctgcg ccgtattttt gtctggcacg
    15481 gttcagtatt atccaagata tcctcgcgct tactactcaa ttttctcttt tctatcgctg
    15541 ttattttcat gctgccctgg tacacgcatt tgggcattaa attcaccctc gcaccgttca
    15601 gcattctcgg tgtcgccatc gccatttttc ttggttttcg taataatgcc ggttacgccc
    15661 gttacgttga agcgcgaaaa ctttgggggc agttgatgat tgcctcgcgg tcgttactgc
    15721 gcgaggtaaa aacgacattg ccggattcgg caagtgtaag ggagtttgcc cggctgcaaa
    15781 tcgccttcgc ccactgttta cgcatgacat tacgcaaaca gccacaggcg gaagtgctgg
    15841 ctcattatct caagactgaa gatcttcagc gtgtactggc ttctaactct ccagctaacc
    15901 acatcttgtt aataatgggg gagtggttgg cggttcagcg acgcaatgga caactttcag
    15961 atatcctgtt tattagcctc aacgatcggc ttaatgatat ttcagcggtc ctggcaggat
    16021 gcgagcgcat tgcctatacg ccaattccct ttgcctacac cctgattttg catcgcaccg
    16081 tgtatctgtt ttgtatcatg ctgccgtttg cgctggtcgt ggacctgcat tacatgacgc
    16141 cttttatctc tgtgctgatt tcctacactt ttatttcgct ggattgtctg gcggaagaac
    16201 tggaagatcc cttcggtact gaaaacaatg atttaccgct ggatgccatc tgcaacgcta
    16261 ttgaaattga cctgttacag atgaacgatg aagcagaaat tccagcgaaa attcttcccg
    16321 atcgccatta ccagctgacg tgataattac tccgtacggc gggcaacaat aaacagacgc
    16381 ggaaatgcca gcaatatctg tccgttctcc tggaggggat attgctcttc cagcatctga
    16441 tggtagcgcg taagaaaatg ctgctgttcg ctctcggtca gatcctgtaa ccacggacgt
    16501 aatccagtgg cggtcaccca atcgataatc gcctggtgcg acggcatctg gtgatagtaa
    16561 gtggttcgcc agatatcgac ctcacatccg gcttcactca gaatatcgta gtaagcatga
    16621 acgccagcca gcggctcacg cccgcgatcc gggtagtttt gttcccaggc aacttcgcgc
    16681 atgagcacat gggtcggctc cagccagtta tctggcatct gtactgccag cacgcccagc
    16741 ggactgagta acgaaaccaa atgaggaaac aattcgtagt ggtcgggcag ccattgcagc
    16801 gaggcattag caaaaatcag atcgagtgcc tgttccggtt gccagttgcg gatatctgct
    16861 tccacaaaca ggcagtctgg caaagcactg cgcgcttcag caatcatcgc cggagatgag
    16921 tcgatgcccg ttatcctggc agcaggccaa cgttggtcta gaagggcggt gctgttacct
    16981 gggccacagc caagatcggc aatgtattcg atattctcca gcggcaccct ggcaagcagc
    17041 tccaccgccg gacgcgatcg ttcagcggca aagtgcagat ataaagaggg gttccagtca
    17101 gacatttttt tactcccgtc agcgcgttac cccaattgta gataaaactg ataaattcgc
    17161 ctcacggcat caccccattg aacagacgtt ttttacgcgg cccggtcatt aagattacag
    17221 attccggtag tcaaagaagc caccgcatta ggatgtgctt ttttaaatcc tcctttttat
    17281 ccgcgatcgc ggatatcgca gcgtttatcc cgtagagcgg ataagatgtg tttccagatt
    17341 gactttatcc tcactaaagg ataaaactga taatatcccc ttaagaggat aaatttgctg
    17401 tggacgtatg acatgatgag ctttcagaag atctatagcc caacgcaatt ggcgaatgca
    17461 atgaaactgg ttcgccagca aaacggctgg acgcagagcg aattggcgaa aaaaatcggc
    17521 attaagcagg cgacaatttc caatttcgaa aacaatcctg acaatacctc gctcacgaca
    17581 ttttttaaga ttttacagtc gcttgaactc tcaatgacgc tatgcgacgc gaaaaatgcc
    17641 tcgccagaag cagcagaaca gcaagatctg gaatggtaat gcctaaacta gtcacttgga
    17701 tgaacaacca gcgggtaggc gagttaacga agttagccaa cggcgcgcac acctttaagt
    17761 atgcacctga gtggttagcc agccgttatg ccagaccgtt gtcactttcg ctgccattgc
    17821 agagggggaa tatcacctct gatgccgtat ttaacttctt cgataacctg ttacccgata
    17881 gcccgattgt acgtgaccgg atcgttaaac gttatcatgc taaatccaga caacatcatg
    17941 ctaaatccag acaaccgttt gatttattgt cagaaatagg gcgagatagc gttggtgccg
    18001 tgacgttaat ccccgaagat gaaaccgtaa cgtgtccgat aatggcatgg gaaaagctta
    18061 ctgaagccag acttgaagaa gtattaacgg cttataaagc agatatcccg ttaggcatga
    18121 ttagagaaga aaatgacttt cgcatctcgg ttgctggcgc acaggagaag actgcgctgc
    18181 tcagaatagg caatgactgg tgctatggct tttttaatgg ggtccagcga ggcgctgaaa
    18241 gatcgctatg attttatgaa gtttcaggtc ttccagtggt tgattggcgc aacagatggc
    18301 catgcaaaaa acttctccgt atttattcag gctggcggca gttatcggct cacaccattt
    18361 tatgacatca tttcggcatt tccggtcctt ggcggtacgg gaatacacat cagcgatctc
    18421 aaactggcaa tggggcttaa cgcatccaaa ggcaaaaaaa cggcaatcga taaaatttat
    18481 ccgcgacatt ttctggcgac agcaaaggtg ctgaaattcc cggaagtgca gatgcatgaa
    18541 atcttgagtg actttgccag aatgattccg gcagcactgg ataacgtgaa gacttcatta
    18601 ccgacagatt ttccggagaa cttggtgacg gcagttgaaa ccaatgtgtt gaggttgcac
    18661 gatcggttaa gccgagaata cggtattaag taagatatgg ggtttttgtc gttgttaatg
    18721 agcaggacaa tcatgaaccg ccaacgactg gattcgccct tggtttatat tggtgcgcat
    18781 aatgtgacat gcgttatgtt gaaaaggccg ctgcgatact ttccaccgca gcggcctttt
    18841 tatacataac gccattgtgc gcactgttgt gcattcagtc gttgcttctg ttgattttat
    18901 tgtgtgccct caggacgatt ggcaacacta ttgttatgac gctgaacaaa aacatgccga
    18961 acagactacc ggcaatcagg tatttcatta gtaaatccag aatcatgtcg tgcatggttt
    19021 ttctgagctg atgttgttaa tcctgtcggg actctacatt cctttagcta ttttttaaat
    19081 cgtgcttcct cttccgtgtg gccgtaacgt tttgttttta cattgtttat tgtgcgatcc
    19141 agattcagca agtctataaa tttttctaga aaaacaatat catctttctc tatgtcccgg
    19201 atcgcggcca ttaggcgtat tttcgccgcc gcttcacctg tttcatgacc ttgtaactta
    19261 aaatctatac cgtaaccaaa ggcagtcata tcttcaccaa ggttctcagg cagaggcagg
    19321 gttgactcac cgaatgctaa ccataccgga ttttttcctg tcgctttagc tatcttcctg
    19381 atttctatta attttggttc cctttctcca gaagccagcc gtttaagggt actgacgctg
    19441 ataccagttt cgttttcgat atcgtcatat gtaaaatttt ggatgataga cagacgtata
    19501 cgttctgcac actctttgtc tactgccata tctgctgcct tctcatgctg attgcaaagt
    19561 cttaatttta acttccattt ttcatagctg aatccaaggg gtcagttttg atatttattg
    19621 gtatcaacat tattgacgtt tgttaagttt gataatagca taactgaaac cgtaaagtat
    19681 cagtgctgag ccttgcggtt catcttgaat gattgagaga ttctatattt tatgaccgaa
    19741 tcagccatcc ttacagcgta taacgccatc gacgcggcat ccgttgccct tggtcgtccc
    19801 tgcctgacgc tgcccgaacg cgagcatttt cggactgagc gcgatgttgc cacccgcaac
    19861 cttgctacgc tttcccgcca gtatcgtgat gaggtcggga ccttatctgc acagctcgca
    19921 ttgccgcgta acacgcaggt tgaccgtgtg atttacggaa ttaactgcac ggatttatct
    19981 ctcgctgaca ggggcagggc tgccggactg gtatcacagt ttggctcgtt ttcgttcacc
    20041 gtggcgcatg ctttctgccg tctggtttac cagctcggtg tgaaaaattc gctgatggcg
    20101 cttgagtccg cgcaccgttt tgctttcttc aatcaggacg gagaccctta tgaattctcg
    20161 cttttctgca ctgatgagca gattgctgtc aacgacggat gaaaagtgat ccacttatat
    20221 ctccaccaac ggcccaatat tgatccaccg ttttactcag gattagcttc tgctataa
//
LOCUS       DAZGJT010000086        19795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZGJT010000086 DAZGJT010000000
VERSION     DAZGJT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.086"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            116..619
                     /gene="tssB"
                     /locus_tag="ACHKFK_003467"
     CDS             116..619
                     /gene="tssB"
                     /locus_tag="ACHKFK_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO9053732.1"
                     /translation="MSKMNNNGGSVAPKERISVRYTPKVDGVAADIELPLNLLITGNL
                     KGKPDNTPLDERTAIAINRYNLNAVISEADIEREFVVPAELSDAPNEQMYVNLKVKSM
                     DDLSPDHIASQVPEIKRLLELREALVALKSPLGNIPAFRAQLQALLENEDTREQLIQE
                     PGIAVQK"
     gene            642..2126
                     /gene="tssC"
                     /locus_tag="ACHKFK_003468"
     CDS             642..2126
                     /gene="tssC"
                     /locus_tag="ACHKFK_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9053733.1"
                     /translation="MSVKEEIAPVSASQTAAPPSLLDEIMAQTRVQPKSESYDITRQG
                     VSAFIAAMLQGDSSAEPINILAVDAMIADIDARTSRQMDAIIHAPEFQELESLLRSLK
                     LLVERADTRENIKVHFLNVTQEELLDDFEFAPEITQSAYYKHVYSSGYGQFGGEPVAA
                     VIGNFAFKNTTPDMKLLKYISQVSAMAHSPFLSSVSSEFFGLDSWTELPGIKEPGAIF
                     EGPAYSRWRALRESEDSRYLGLTAPRFLLRHPYSPDENPVKTFRYHEDVSQSHESYLW
                     GNTSFLLAANLAESFAKYRWCPNIIGPSSGGAVKDLPVHLYESMGQMQAKIPTEVLIT
                     DRREYELAEEGFITLTMRKGSDNACFFSANSVQKPKTFPKTPEGKAAETNYKLGTQLP
                     YLFVISRLAHYIKVIQREQLGSWKERSDLERELNTWIRQYVADQENPPAEVRSHRPLR
                     QAKIEVLDVDGEPGWYQVAISVRPHFKYMGASFDLSLVGRLDKE"
     gene            2131..2556
                     /gene="tssE"
                     /locus_tag="ACHKFK_003469"
     CDS             2131..2556
                     /gene="tssE"
                     /locus_tag="ACHKFK_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9053734.1"
                     /translation="MLRRDSGPTGSLFERIREAANPPSYQNPKEALIRSIRRNLRQVL
                     NTRSGSCYGSPELGITDLNDESLASSDFRREIRKSISQCILHYEPRITDVVVTAAAPD
                     EYAPVELRFHIVATIDVSETRGVFEFDILLDNHQRYCVE"
     gene            2562..4403
                     /gene="tssF"
                     /locus_tag="ACHKFK_003470"
     CDS             2562..4403
                     /gene="tssF"
                     /locus_tag="ACHKFK_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9053735.1"
                     /translation="MAFEERYYREELDYLRQLGKLLAQEKPHLAHFLAEKEGDPDVER
                     LMEAFAFMSGSLRQKLEDEFPEFTHGLIRMLWANYLRPVPAMTVIAYEPKTDQLKVPV
                     QVCRNELIRSRSENSSLTAQKVLPDNHDKIVSSVACHFTLARDIWLQPLRILDTRNAS
                     SLKEGIIDISFTADNNVSPAMLDLNKITFWLGNEDDYTRHQLYLWFCECLMDAELIAG
                     EYRLPLPDLWLDAAGFDNRDALLPWPKNVHSGYRVLQEYFCYPEAFFFFHLRDVAPLP
                     DDFPVNAFTLRLHFNRPLPADIKLRQDSLRLHCTPAINLFTHYAEPVRPDGRMAEYPL
                     RASHKHPDAYDIFQVSTVTSKVKVSGTEISPGSQARVWPEFESFQHQMEYSRQREVVY
                     WHHRTKTSLFHYGLEHSIAFVHADGSIPDSSRFNDDVITTSLICTNRMIPARLHTGDI
                     CVAVNKNPAVASFRNVTRPTLPLWPVTDGDMHWSLISAMNLNYLSLLDRETLIQILRT
                     FDLPGAHHPQRARLSRQKLDAIEKLETKPVDRLFKGVPVRGLATTLWIRPDPFICEGE
                     IYLLGTVLSHFFALYASINSYHCLKIINTESQESWEWQEKMGQHALI"
     gene            4367..5416
                     /gene="tssG"
                     /locus_tag="ACHKFK_003471"
     CDS             4367..5416
                     /gene="tssG"
                     /locus_tag="ACHKFK_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9053736.1"
                     /translation="MAGENGPARPDIATKPPLPADVRNCNFYVLMEALYRRHGAPGQD
                     ISLRTEPAREIVRFSSDASISFPGTDLSALSRSQNGQYVLQTRFLGFSGSQSPLPGYY
                     LDQMAQESAQNEDGLKEFRDLFSHRWTQFAYHAWRKYRYYICFRSGGTDTFSQRMYAL
                     VGLGNQSVRDRLAINHSKMLAYAGILATPGRAPEVICNLVSHCFDLPDVTIERWQLRK
                     VAIDPAHQNRLGVRNPKGKTAGHIPGRSILGVNFTLGARVPDRSGKFLLQIGGLSRER
                     YLSFLPDGENHLPLTMFLSFILRDQFAWDLRLCLAPQQAKGMRLGDPASSRLGRTSFI
                     GQPKVPPAITIRIRE"
     gene            5433..6713
                     /gene="tagH"
                     /locus_tag="ACHKFK_003472"
     CDS             5433..6713
                     /gene="tagH"
                     /locus_tag="ACHKFK_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001511117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO9053737.1"
                     /translation="MAEEKQQSRQASLTLQVMNGNELESGRAAKCLFSENGGDIGHTP
                     ECHWQVQDRAGSIAARACTVIRHDGAYCLRCLTPGLMINLAPASSDALIRLRQGDEIQ
                     LGALALKVFLHDGAAVTYDERMATPETIVMNRDSLADTLLTTEGQPAYPGMPFQHQLA
                     PTVAHGFSSDPLQALQTESLMVADDPIAPRVSRPVAPASDLTGANGINTPFMDLPPGN
                     ARHEDGDEFSAMAQYHLAVTPLLRGMECPLTLHNSQDADEFLEEAGRALQAAIKGLLS
                     LQQQQNSLSDKHLRPLEDNPLRLDMDYATALNVMFAEGKSPVHLAAPAAIAESLRNIR
                     HHEEANRAAIVEALRVMLDAFSPGNLMRRFAQYRRSHELRQKMDNAWAWQMYSNYYDE
                     LASSRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            6710..7234
                     /gene="tssJ"
                     /locus_tag="ACHKFK_003473"
     CDS             6710..7234
                     /gene="tssJ"
                     /locus_tag="ACHKFK_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9053738.1"
                     /translation="MNGKAFLACVLMSVVLTGCETAKKISQVIRNPDIQVGKLMEQST
                     ELTVTLLTEPDSNLTADGEAAPVDVQLVYLSDDSKFQAADYDQIATTALPDVLGKNYI
                     DHQDFNLLPDTVKTLPPIKLDEKTGYIGVIAYFSDDQATEWKQIEPVESIGHHYRLLV
                     HIRASAIEMKKEEN"
     gene            7237..8583
                     /gene="tssK"
                     /locus_tag="ACHKFK_003474"
     CDS             7237..8583
                     /gene="tssK"
                     /locus_tag="ACHKFK_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9053739.1"
                     /translation="MATMKNKVIWQEGLFALPQHFQQQQRHCEYLLTRRLDAQGDFFW
                     GFTELSINTELLAQGKIMIDRAAGCMPDGTVFSIPDQDLLPEPFQPGTLSSKESHNIY
                     LALPVISDVINEIQGLHSAGQGTERYRLTHTRVRDFHTDEGDEQPVGLGQLIPRIVSG
                     ADDLSAMVTLPLCRILNKNATGALVLDNTFIPTIQAVHVSGLLGAFSGEVQGLLATRA
                     ADLAGRIGSPEQSGIADVAEFMMLQMLNRYQMQFTHRSQLHTLHPEAFYRDLVGLLGE
                     LMTFTEGNRLPCTVEPYNHRDLTATFTRTVIPELRRALNTVLVPRAQNLPLFFSEGTW
                     IATINDPTLLQSCKLVLAVRARMPYDQVQRQFIQQSKVAATDKIRSVVSVQVPGIPLH
                     TLTAAPRQLPYHEGYVYFELEKGTPAWQDVIKAGALALHISGSFPDLNLQLWAIRG"
     gene            8588..9352
                     /gene="icmH"
                     /locus_tag="ACHKFK_003475"
     CDS             8588..9352
                     /gene="icmH"
                     /locus_tag="ACHKFK_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO9053740.1"
                     /translation="MSDMSEASVFPVEPESGTPGRQYRLALRGNSLNPMIDAATPLLG
                     MVMRLSTMNSQAMPEHLFDQVVTDVQAVEQLLQEQGYEPGVIVSFRYILCTFIDEAAL
                     GNGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPVRYQDLLEFLYLCFSLGF
                     RGRYKVAIQQQDEFERIYQRLHHALHKLRGDAPFPLLHQKNNIQGGRYQLIRRLTIKH
                     VLCGGLVVLTVFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9362..12034
                     /gene="tssH"
                     /locus_tag="ACHKFK_003476"
                     /gene_synonym="clpV"
     CDS             9362..12034
                     /gene="tssH"
                     /locus_tag="ACHKFK_003476"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9053741.1"
                     /translation="MIQIDLATLVKRLNPFAKQALEMAASECMSQQASEITVAHVLLQ
                     MLAIPRNDVRVIAERTGISAEDLRQALTVESYPGGRSAEGYPSFSPMLIEWLKESWLL
                     ASAQMQHSELRSGVLLLTLLHSPLRYIPPAAARLLTAINRDQLQQDFAAWTKESAESV
                     NQVGGQAPGTPETGDSGLARYAKNMTEDARNGKLDPVLCRDHEIDLMIDILCRRRKNN
                     PVVVGEAGVGKSALIEGLALRIVSGQVPDKLKNTDIMTLDLGALQAGASVKGEFEKRF
                     KGLMAEVISSPVPVILFIDEAHTLIGAGNQAGGLDVSGLLKPALARGELKTIAATTWS
                     EYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAAAT
                     LSERYLSGRQLPDKAIDVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLEREL
                     RIGLRTDTSRMTEVLEQYDETLSALDELEVAWQQQQQTLVQEIIALRQQLLGAAEDTD
                     AVVEEVTETVSPVQRLAQLTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLS
                     QNEMSVITDLPKWLGDTIKGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVG
                     KTETVLQLAELLYGGRQYLTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQ
                     KPYSVVLLDEVEKAHPDVLNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIV
                     EHADDPETMQEALYPVLADFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLR
                     SRFGADVIIGPEVTDEIMSRVTRAENGARMLESVIDGDMLPPLSLLLLQKMAANTAIA
                     RIRLSAADGAFTADVEDALPDDAVTPQTEDETVL"
     gene            12031..12774
                     /gene="vasI"
                     /locus_tag="ACHKFK_003477"
     CDS             12031..12774
                     /gene="vasI"
                     /locus_tag="ACHKFK_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO9053742.1"
                     /translation="MSRLCVFRQTGWFIAGLMAGLPASAAPTETSSVAGVAVAVATTT
                     PPDATATLQAMQSCRQETAALERLDCYDRILAPEQAGFGGAALVKARYQGEAWARATE
                     QEKRRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADSRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGTD
                     NAAGKLTFNIDGLARVIAPLRDACHWAGE"
     gene            12779..14221
                     /gene="tssA"
                     /locus_tag="ACHKFK_003478"
     CDS             12779..14221
                     /gene="tssA"
                     /locus_tag="ACHKFK_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO9053743.1"
                     /translation="MAMDLRNPDVWLAHLLENLPEDKLSAALDDGNADWEFVDSEIVK
                     LGSLAHSQLDIPELQRRGLMLLASETKDFRLLAHLLRTLQHAGDILLASRMLAQYTEH
                     YWTCAAPQNMAHKKRFAAQVIKRFESAVQDFAGNAATIQRDALLGELAKLAQCWQAHN
                     ASELAKATDDLFSLFQRAFRDVAPEMTSSARSAAIAPQATTGYVPDMSPHTVAAQPPV
                     AATPIPQVTVESHDDKAWRDTLLKVAAILCERQPESPQGYRLRRHALWQTITSTPQAE
                     SDGRTPLAAFPVDMMDDYLARLNNADMALWQQVEKSLLLAPYWLDGHYLSAQTALRLG
                     YKQVADAIRDEVTDFLARLPALINLLFNDRTPFVSEQTKQWLASSGSVNQTVPVVQTD
                     EELQAAKACFDENGLEAALRYLENLPEGDPRHQFHRQFFGAQLLEEAGMVQLAQQQYR
                     MLFRTGLHMMLSEWEPSLLKALEQKLTAEQ"
     gene            14301..17765
                     /gene="tssM"
                     /locus_tag="ACHKFK_003479"
     CDS             14301..17765
                     /gene="tssM"
                     /locus_tag="ACHKFK_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO9053744.1"
                     /translation="MPRFKVSATWLLTLAWIFLLVWIWWQGPKWTLYEQHWLAPLANR
                     WLATAVWGLIALVWLTWRVMKRLQKLEKQQKQQREEEKDPLTVELHRQQQYLDHWLLR
                     LRRHLDNRRYLWQLPWYMVIGPAGSGKSTLLREGFPSDIVYTPESIRGVEYHPLITPR
                     VGNQAVIFDVDGVLTTPGGDDLLRRLREHWLGWLMQTRARQPLNGLILTLDLPDLLTA
                     DKSRRETLVQNLRQQLQEIRQSLHCRLPVYVVLTRLDLLNGFAALFHSLDKKDRDAIL
                     GVTFTRRAHESDGWRSELGAFWQTWVQQVNLALSDLVLAQTGAAPRSAVFSFSRQMQG
                     TGEIVTALLAALLDGENMDVMLRGVWLTSSLQRGQVDDIFTQSAARQYGLGNSSLATW
                     PLVETTPYFTRRLFPEVLLAEPNLAGENSVWLNSSRRRLTAFSTCGAALAALMVGSWH
                     HYYNQNWQSGVNVLAQAKAFMDVPPPQGTDEFGNLQLPLLNPVRDATLAYGDYRDHGF
                     LADMGLYQGARVGPYVEQTYIQLLEQRYLPSLMNGLIRDLNIAPPESEEKLAVLRVVR
                     MMEDKSGRNNEAVKQYMARRWSNEFHGQRDIQAQLMVHLDYALEHTDWHAQRQSSDSD
                     AVSRWTPYNKPVINAQHELSKLPIYQRVYQTLRTKALSVLPADLNLRDQVGPTFDNVF
                     VAGNDEKLVIPQFLTRYGLQSYFIKQHDGLVELTALDSWVLNLTQSVAYSEADREEIQ
                     RHITEQYLSDYTATWRAGMDNLNVRDYETMPALTDALEQIISGDQPFLRALTALHDNT
                     HALTLSGKLDDKAKEAAINEMDYRLLSRLGHEFAPENSALEEQKDKASTLQAVYQQLT
                     ELHRYLLAIQNSPVSGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGKLAD
                     QAWHVVMVEAVRYMEVDWRDNVVKPFNEQLADNYPFNPRATQDASLDSFERFFKPDGI
                     LDNFYKNNLRLFLENDLTFGDDGRMLIREDIRQQLDTAQKIRNIFFSQQNGLGAQFAV
                     ETVSLSGNKRRSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESQLTLIGTSGRAPR
                     SIAFSGPWAQFRLFGAGQLTNVTSDTFNVRFNVDGGAMVYRVHVDTEDNPFTGGLFSQ
                     FRLPDTLY"
     gene            17776..19134
                     /locus_tag="ACHKFK_003480"
     CDS             17776..19134
                     /locus_tag="ACHKFK_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO9053745.1"
                     /translation="MSNNALTQTIVTGSDPRALPEFSAIREEINKANHPSQPELNWKL
                     VESLALSIFKANGVDLHTATYYTLARTRNQGLAGFCEGAELLAAMIIHEWDKFWPQSG
                     PARTEMLDWFNTRTGNILRQQVSFSENDLSLLYRTERALQLICDKLQQVELKRQPRVE
                     NLLYFVQNTRKRFEPQPRNRTDTAAQTMVRTLVYAPEGTASATAETMPPLPDLPEMRV
                     GVRGVADNADKAKQGDTIKGFVAGAACTAVIASALWWWQAYPMQQQLTQVRDTTQGAA
                     TVWLASPVLKEYEQYLQQLLNVPPLQPLETGMQMMRTADTLWPESLQQQEASRMWSNT
                     LRNRAQASPQMKGWQQARQNLRDFADLMMKKETEKQGFTLSYIKTVTWQAERLLNQET
                     PLEYLLTQYQETRTQKQDTQALEKEINERLDGLLSRWLLLKNTGQDMATDNRTEPVHP
                     TH"
     gene            19152..19631
                     /locus_tag="ACHKFK_003481"
     CDS             19152..19631
                     /locus_tag="ACHKFK_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9053746.1"
                     /translation="MANPVYLTLNGELQGLISSGCSSMPSIGNKAQIAHQDQIMVTSL
                     SHGLSRAQNVNHQELTITKPVDKSSPLLGKAISENECLTCDFVFYRTNRFGINEPYYK
                     LKLANARLSNIGLTVPHTINDSPGQAEESVSFTYESINWEHSVAGTSAYSLWSERIF"
BASE COUNT         4619 a         5545 c         5486 g         4145 t
ORIGIN      
        1 ataccgatca caacagaagg aaggttacat tatctaactg ccactgaaac ggtattgtcg
       61 gtatttgtat gcagtacgaa aatgctgtgc tcatggcctg aacgggaaca tttttatgag
      121 caaaatgaac aacaatggcg gcagtgtcgc gccgaaagaa agaatcagcg ttcggtatac
      181 accaaaagtt gatggggtgg ctgctgatat cgaattgccg ctgaatttat tgatcaccgg
      241 caatctgaaa gggaaaccag ataacacgcc gttggatgag cgaactgcca ttgctatcaa
      301 ccgctataac ctcaacgcag tcatttcaga agctgacatt gaacgggagt ttgttgttcc
      361 ggctgaactc agtgacgctc ccaatgagca aatgtacgtc aacctgaaag tcaaatctat
      421 ggatgacctt tctcctgacc atattgccag ccaggtacca gaaataaaac gtctgctcga
      481 attgcgtgaa gccctggtcg cacttaagtc tccactaggc aatattccag ccttccgcgc
      541 acaacttcag gcgctactgg aaaacgaaga cacccgcgag cagctgatcc aggaaccggg
      601 tatcgccgtt cagaaataat tttcacagaa ggatattcag tatgtcagta aaggaagaaa
      661 ttgctcccgt cagcgcgtct caaaccgccg cgcccccatc tctgcttgat gaaatcatgg
      721 cgcaaacccg cgtccagcct aaatcagaaa gctacgatat cacccgccag ggcgtgagcg
      781 cctttattgc cgccatgtta cagggcgaca gtagcgctga acctatcaat atcctggccg
      841 ttgacgccat gattgctgat attgatgccc gaaccagcag gcagatggat gccattattc
      901 atgccccgga gtttcaggaa ctggaatccc tgctgcgctc gctgaagctg ctggttgaac
      961 gcgcggatac acgggaaaac atcaaagtcc atttcctgaa tgtcactcag gaagaactgc
     1021 tggatgattt cgaatttgca cctgaaatca cgcagtctgc gtattacaag catgtatatt
     1081 caagcggtta cggacaattt ggcggtgagc cagtggcggc ggtaatcggc aactttgcct
     1141 ttaagaacac cacgccggat atgaagctgc tgaaatatat cagccaggtc agcgcgatgg
     1201 cgcacagccc gttcctgtcg tcagtatcat ccgaattttt cggtctggat tcatggacag
     1261 agttaccggg gatcaaagaa ccgggtgcca tctttgaagg cccggcctac tcccgctggc
     1321 gcgccctgcg tgaatcggaa gattcccgtt acctgggcct gaccgccccc cggttcctgc
     1381 tgcgccaccc ttactcaccg gatgaaaacc ctgtcaaaac cttccgttac catgaagatg
     1441 tcagccagag tcatgagtct tatctgtggg ggaatacctc gtttttactg gccgccaacc
     1501 tggcggaaag ttttgctaag tatcgctggt gcccaaatat tattggccct tccagcggcg
     1561 gcgcagtcaa agacctcccg gtgcatctgt atgaatccat ggggcaaatg caggcaaaaa
     1621 ttccgactga agtgttgatc actgaccgga gagagtatga gctggcggaa gagggcttta
     1681 tcacactgac catgcgtaaa gggtctgata atgcgtgctt tttctcggcc aactcggtgc
     1741 agaaacccaa aacattcccc aaaacccccg aaggcaaggc tgcggagacc aactataaac
     1801 tcggcaccca gctgccatac ttgtttgtta tcagcaggtt agcgcattac atcaaggtaa
     1861 ttcagcgcga acagcttggc tcatggaaag aacgtagtga cctggagcgt gagctgaaca
     1921 cctggattcg ccagtatgtt gctgaccagg aaaatccgcc tgccgaagtc cgcagccacc
     1981 ggcctttgcg tcaggctaag atcgaggtac tggatgtgga tggtgagccg ggctggtacc
     2041 aggtcgcgat ttctgtcaga ccgcatttta aatacatggg tgccagtttc gacctgagcc
     2101 tggtcggacg actggataag gaataatccg atgctaagac gggattcagg accaaccggc
     2161 agtctttttg aacgtatccg tgaggcggca aatccgcctt catatcagaa tccaaaagaa
     2221 gcactgatac gttccatcag acgtaacctg cgacaggtcc taaatacccg ctccggcagt
     2281 tgttatggct caccggagct ggggattact gatttaaatg atgaatcgct ggcctccagc
     2341 gatttcagac gagaaatccg taagtccatt agccagtgca ttttacatta cgaacctcgt
     2401 attaccgatg ttgttgttac ggctgcggca ccggatgaat acgcccctgt ggaactgcgt
     2461 tttcatattg tggctacgat cgatgtcagc gagaccaggg gagtgtttga atttgacatt
     2521 ttgctggaca accatcaacg ctattgtgtg gagtgatctc tgtggctttt gaagaacgtt
     2581 actaccgcga agagttggat tatctgcgcc agcttggcaa actgttggcg caggaaaaac
     2641 cgcatctggc ccattttctg gccgagaaag agggtgatcc ggatgtggag cgactgatgg
     2701 aagcctttgc ctttatgtcc ggtagcctgc gccagaaact tgaagacgaa tttcccgaat
     2761 ttacccatgg gctgatccgt atgctgtggg caaactattt acgcccggtt ccggccatga
     2821 cggttattgc ctatgaaccc aaaaccgatc agttaaaagt ccctgttcag gtctgtcgta
     2881 atgaactgat cagaagccgc tcagaaaact cctccctgac tgctcagaag gtgctgcctg
     2941 acaatcatga taaaatcgtt tcttccgtag cctgccattt cacgctggcg cgcgatatct
     3001 ggctgcaacc actgcgcatt ctcgataccc gcaacgccag cagcctgaaa gaaggcatca
     3061 tagatatcag ttttaccgca gataacaatg tctcccccgc catgcttgac ctgaacaaaa
     3121 tcacgttctg gctgggtaat gaagacgatt atacccgaca ccagctttat ctgtggtttt
     3181 gcgaatgcct gatggatgcg gaactgattg ccggagagta ccgcctgccg ctgccggatc
     3241 tgtggctgga tgctgcgggt tttgacaacc gggatgctct gctgccgtgg ccgaaaaatg
     3301 tccacagcgg ctaccgcgtg cttcaggagt atttctgcta tcccgaagcc ttcttctttt
     3361 tccatctgcg cgatgttgcg ccgctaccag acgatttccc ggtcaatgcg tttacgctgc
     3421 gcctgcactt taaccgcccg ttacctgccg acatcaagct gcgccaggat tcgctgcgtc
     3481 tgcactgtac gccagccatt aacctgttta cacattatgc ggaacccgtc aggccggatg
     3541 ggcggatggc ggaatacccg ctgcgcgcca gccataagca tccggacgcc tatgacattt
     3601 ttcaggtcag cacagtcacc agtaaagtca aagtttcagg tactgaaatc agcccaggta
     3661 gccaggcgcg tgtctggccc gaatttgaaa gcttccagca ccagatggag tacagccgcc
     3721 agcgggaagt cgtctactgg catcaccgga ctaaaacgtc cctgtttcac tacgggcttg
     3781 aacatagtat cgcctttgtc catgcagacg gtagcattcc ggacagctca cggtttaacg
     3841 atgacgtgat caccacgtca ttaatctgta ccaaccgtat gatccctgcc cggctgcata
     3901 ccggtgatat ctgcgtggcc gtcaacaaaa atccggcagt ggcctcattt cgtaatgtca
     3961 cgcgcccgac actgccgctc tggccagtca cggacggcga catgcactgg tcgctgattt
     4021 ccgcgatgaa cctgaactac ctctccctgc tggacaggga gacactgatc cagatcctgc
     4081 ggaccttcga cctgccaggt gctcaccatc cgcagcgggc cagactgtca cgccagaaac
     4141 tggatgccat agaaaaactc gaaaccaaac ctgttgacag gttgtttaaa ggcgtaccgg
     4201 ttcgcgggct ggcaaccacg ctgtggatac gtcccgatcc gtttatctgc gaaggtgaaa
     4261 tttatcttct gggtaccgtg ctctcgcact tctttgccct ctacgccagc atcaattcgt
     4321 accactgtct gaaaatcatt aacacggaaa gtcaggagtc ctgggaatgg caggagaaaa
     4381 tgggccagca cgccctgata tagcgacaaa accgccgtta ccggcggatg tgcgcaactg
     4441 caatttctat gtgctgatgg aggcgctgta ccgtcggcat ggcgcgccgg gccaggacat
     4501 ttccctgcgt accgagcctg cccgggagat cgtgcgtttc agctctgacg ccagtatcag
     4561 ttttccgggt acggacctga gtgcgctttc ccgtagccag aacggacagt atgtactgca
     4621 aacccggttc ctgggttttt ccggcagcca gtcgccgcta ccgggctatt atctggatca
     4681 gatggcgcag gagtccgcac agaacgaaga cggcctgaaa gaatttcgcg atctattcag
     4741 ccaccgctgg acacagtttg cctatcatgc ctggcgcaaa taccgctact acatctgctt
     4801 tcgtagcggc ggtactgaca ccttttcaca gcgcatgtac gcactggtcg ggctgggtaa
     4861 tcagagcgtg cgtgacaggc ttgccattaa ccacagcaaa atgctggcct acgccggaat
     4921 tctggcaaca ccaggccgcg cgccggaggt tatctgtaat ctggtgtcgc actgttttga
     4981 tttgccggat gtcaccattg aacgctggca actgcgtaaa gtcgccattg atccggcaca
     5041 ccagaaccgg ctgggtgtac gtaatccgaa aggaaaaacg gctggacata ttccgggacg
     5101 ctccatactc ggcgttaatt ttaccctcgg cgcacgggtg ccggatcgca gcggaaaatt
     5161 cctgttgcag attggcggcc tgtcgcggga gcgttatctc tcatttttac ccgatggcga
     5221 aaatcacctg ccgctgacca tgttcctttc ctttattctg cgcgaccagt ttgcctggga
     5281 tttacggctg tgcctggcgc cacagcaggc taaagggatg cgccttggcg atccggccag
     5341 ctcccgcctc ggcagaacca gctttattgg tcagccgaaa gtaccgcccg ccatcacaat
     5401 ccgaatcagg gaataattct atggaaccac ttatggctga ggaaaaacag caatcccgcc
     5461 aggcctcact gactttacag gtgatgaatg gcaacgaact ggaaagcgga cgcgccgcaa
     5521 aatgcctgtt cagcgaaaac ggcggtgata ttggccatac gccggaatgt cactggcagg
     5581 tacaggaccg ggcgggaagc attgctgcgc gtgcctgtac cgttatccgg cacgacggag
     5641 cctattgcct gcggtgcctg acgccgggac tgatgattaa tctggccccg gcctcttccg
     5701 atgcgctcat ccgcctgcgc cagggggatg agatccagct cggcgcactg gcgctgaaag
     5761 tgtttctgca tgacggtgca gctgttacct atgacgagcg gatggcgacc ccggaaacga
     5821 ttgtaatgaa ccgcgacagt ctggcggaca ccctgctgac caccgaggga caaccggcgt
     5881 atccgggaat gccgttccag catcagcttg cccccacggt ggcgcacggt ttttccagcg
     5941 atccgctcca ggcactccag acggaaagcc tgatggtggc agatgatccg atcgccccac
     6001 gcgtttcccg gcccgtagcc cccgcatccg atctgacagg tgcaaacggg atcaacacgc
     6061 cgtttatgga tctgccgccc ggcaatgccc gtcatgagga cggcgatgaa ttttctgcca
     6121 tggcgcagta ccacctggcc gtaaccccgc tgctgcgcgg tatggagtgt ccgctgacgc
     6181 tgcacaattc tcaggatgcg gatgagtttc tcgaagaggc cggacgggcg ttgcaggccg
     6241 ccattaaggg attgctgagt ctgcaacaac aacagaatag cctgtcggat aaacacctgc
     6301 gtccactgga agacaacccg ttgcgccttg atatggacta tgccaccgcg ctgaacgtca
     6361 tgttcgccga gggtaaaagc ccggtacatc tcgcagcacc tgcggccatc gccgaaagcc
     6421 tgcgcaacat ccggcatcat gaagaggcca accgggcggc gattgtggaa gcgttgcgtg
     6481 tcatgctgga cgccttctcg ccgggcaacc tgatgcgccg ctttgcgcaa taccgccgca
     6541 gccatgagct gcgccagaaa atggacaacg cctgggcatg gcagatgtac agcaactatt
     6601 acgatgaact ggcctccagc cgacagcagg gatttgaaat gctgttcaat gaagtttatg
     6661 cccaggtcta tgaccgggtg ctgcgcgaaa aacagcggga gccggaagca tgaacggcaa
     6721 agcgtttctg gcctgcgttc tgatgagcgt tgtgttaact ggctgtgaaa cagcgaaaaa
     6781 aatcagccag gtgatccgca atccggatat tcaggtcgga aagctgatgg agcagtcaac
     6841 cgaactgacc gtcacgctgc tgaccgagcc ggacagcaac ctgacggcgg acggcgaagc
     6901 cgcgccggtg gatgtccagt tggtgtatct gagcgacgac tccaaattcc aggccgccga
     6961 ttacgatcag attgccacca ccgcgctgcc cgacgtgctg gggaagaact atatcgatca
     7021 ccaggacttc aacctgttgc cggacaccgt aaaaacactg ccgccgatca agctggatga
     7081 gaaaaccggt tatatcggtg tcattgccta tttttcagac gaccaggcca cagaatggaa
     7141 acaaattgag ccggtagaaa gtatcggcca ccactatcgc ctgctggtgc atatccgcgc
     7201 cagtgcgatt gagatgaaaa aagaggaaaa ctgacgatgg caacaatgaa aaacaaggtg
     7261 atctggcagg aagggctgtt tgccctgccg cagcattttc agcaacagca gcgtcactgc
     7321 gagtacctgc tgacccgtcg tctggatgct cagggtgatt ttttctgggg ctttacggaa
     7381 ctctccatca ataccgaact gcttgcccag ggcaaaatca tgattgaccg tgcggcgggc
     7441 tgtatgcctg acggcaccgt attcagcatt cccgaccagg atctgctgcc ggaacctttc
     7501 cagccgggaa ccctttcatc gaaagagagc cacaatattt atctggcgct gcctgtaatc
     7561 agcgacgtga tcaatgaaat ccaggggttg cacagcgccg gtcagggtac ggagcgttac
     7621 cgcctcaccc atacccgcgt ccgtgatttt cacactgacg aaggcgacga acagccggtc
     7681 ggtctggggc agttgatccc caggatagtc agcggcgcag atgatctcag cgcgatggtg
     7741 accctccccc tgtgccgtat cctcaataaa aatgctaccg gcgcactggt gctggacaac
     7801 acctttattc ccaccattca ggccgtccat gtcagcgggt tgctcggggc attctccggc
     7861 gaggtgcagg gactgcttgc cacccgcgcc gctgacctgg ccgggcgcat tggttcgcct
     7921 gaacagagcg ggatcgccga tgtggccgaa tttatgatgt tgcagatgct taaccgctat
     7981 caaatgcagt tcacgcatcg ttcccaactg cacacgctgc atccggaagc cttctaccgg
     8041 gatctggtgg ggctgctggg agaactgatg acctttaccg aaggtaatcg cctgccctgc
     8101 accgtggagc cgtataatca ccgggatctt accgcgacct tcaccagaac cgtgatcccg
     8161 gaactgcggc gcgcgctcaa taccgtactg gtgccgcgtg cgcagaatct gccgttgttc
     8221 ttttcagagg ggacctggat cgccaccatc aacgatccca cattgttgca gtcctgcaag
     8281 ctggtactgg cagtgcgcgc caggatgccc tacgaccagg tacaacgcca gttcatccag
     8341 cagtcgaaag tggccgctac cgacaaaatc cgcagcgtgg tcagcgtgca ggtgccgggt
     8401 attccgctgc acacgcttac cgctgcgcca cgtcagttgc cataccacga aggttatgtt
     8461 tacttcgaac tggaaaaagg caccccggcc tggcaggacg tgataaaagc gggcgcactg
     8521 gcgctgcata tctccggcag cttccccgat ctgaacctcc agctctgggc tataagggga
     8581 taagtgaatg agcgatatga gtgaagcgtc ggtttttccc gtggagccgg agagcggaac
     8641 accaggacgc cagtaccgtc tcgcccttcg cggcaacagc ctgaatccaa tgattgacgc
     8701 cgccacgccg ttgctcggca tggtgatgcg tctgtccact atgaacagtc aggccatgcc
     8761 ggaacatctg tttgaccagg tagtaaccga tgtccaggcc gtagagcaac tactccagga
     8821 acagggatac gaaccgggcg tcattgtttc gttccgttac attctctgca cgtttattga
     8881 tgaagcggcg ctgggcaacg gctggtccaa caaaaacgaa tggatcaaac agtcgctgct
     8941 ggttcatttt cataacgaag cctggggcgg cgagaaagtg tttatcctgc tcgaacgcct
     9001 gatccgggag ccggtacgtt accaggatct gctggaattt ttatacctct gtttttcgct
     9061 cggctttcgc gggcgctaca aggttgccat tcagcaacag gatgaatttg agcggatcta
     9121 ccagcgcctg caccatgccc tgcacaaact gcgcggtgac gcgccatttc cgctacttca
     9181 tcagaagaac aacatacagg gtgggcgtta tcagttaatc cgacgactga ccatcaagca
     9241 tgtactgtgc ggcggtcttg ttgtgctgac cgtattttat ctgttttatc tgctgcgtct
     9301 ggacagccag acacaggata ttctgcatca gctcaataaa ttactccgat aaggacatct
     9361 tatgatccag attgatttag ccacgctcgt aaagcggctt aacccctttg caaaacaggc
     9421 gctggaaatg gctgcctcgg agtgtatgag ccagcaggcc agtgagatca ccgttgccca
     9481 tgtgctgtta cagatgctgg caatcccgcg caacgacgta agggtgattg ccgaacgcac
     9541 aggcatcagt gcggaggatt tacgccaggc gctgaccgtg gagagttatc ccggtggacg
     9601 ttccgccgaa ggctatccca gcttctcccc gatgctgatc gaatggctca aggagtcatg
     9661 gctgctggcc tcagcccaga tgcagcacag cgaactgcgc agcggcgtcc tgctgcttac
     9721 tctgctgcat tcaccgctgc gctatattcc acccgctgct gcccgtctgc tgacggccat
     9781 aaaccgcgac cagttacagc aggatttcgc agcatggaca aaagaatccg ccgaatccgt
     9841 gaaccaggtt ggcggacagg cacccggcac gcctgaaacc ggcgacagcg ggcttgcccg
     9901 ctacgccaaa aacatgaccg aagatgcccg taacggcaaa cttgaccctg tactgtgccg
     9961 cgaccatgaa atcgacctga tgatcgacat tctctgccgc cgtcgtaaaa acaatccggt
    10021 ggtggtgggt gaagcgggtg tgggtaaaag tgccttaatc gaagggctgg cgctgcgtat
    10081 tgtctcaggc caggtgccgg acaagctgaa aaacaccgat attatgaccc ttgacctcgg
    10141 cgcattgcag gccggggcgt cagtgaaggg cgagtttgaa aaacgcttta agggactgat
    10201 ggcggaagtt atctcgtctc ctgtgccggt cattctgttt atcgacgaag cgcataccct
    10261 gattggcgcg ggaaatcagg ccggaggact cgatgtttcc gggttgctga aaccggcgct
    10321 ggcgcggggt gaactgaaaa ccatcgccgc caccacctgg agcgaataca aaaaatactt
    10381 cgagaaagat gcagctttgt cgcgccgctt ccagttagtg aaggtcagcg aacccaacgc
    10441 tgccgaagcc accattattc tgcgcggttt gtcggcggtc tatgaacagt ctcacggcgt
    10501 gctgattgat gatgacgcct tgcaggccgc tgcgacactg agcgagcgtt atctctccgg
    10561 gcggcagttg ccggacaaag ccattgatgt gctggacacc gcctgcgccc gtgtggccat
    10621 caacctgtca tcgccgccaa aacaaatctc ggcactgacc accctgagcc accagcagga
    10681 ggcagaaatt cgccagcttg agcgcgagct tcgcatcggg ctgcgtaccg acacctcacg
    10741 aatgactgag gtgttggagc agtatgatga aacgctgtcg gcgctggacg aactggaagt
    10801 cgcctggcag cagcagcagc agacgctggt acaggaaatt atcgcgctgc gccagcagtt
    10861 gctgggcgcg gcagaagaca ctgatgctgt ggtggaagaa gtcaccgaaa cagtttcccc
    10921 ggtgcagcgg ctggcacagc ttactgccga actggacgcc ctgcataacg accggttgct
    10981 ggtttccccg cacgtcgata aaaaacagat tgcggcggtg attgccgaat ggaccggcgt
    11041 accgcttaac cgcctgtcgc agaatgagat gtcggtcatc accgacctgc cgaagtggct
    11101 tggtgacacc atcaaaggcc aggacctggc aattgccagc ctgcataaac acctgctgac
    11161 cgcacgcgcc gacctgcgcc gtccgggacg cccgctcggc gcgttcctgc tggctggccc
    11221 cagcggcgtg ggtaaaaccg aaaccgtcct gcaactggca gagctgctct acggcggtcg
    11281 ccagtacctg accaccatca atatgtctga gttccaggag aaacacaccg tttcgcggct
    11341 gattggctct cctccgggtt atgtcggcta cggtgaaggc ggcgtactga ccgaagcgat
    11401 tcgccagaaa ccgtactcgg tggtgctgct cgatgaagta gaaaaagcgc acccggatgt
    11461 actcaacctg ttctaccagg cgtttgacaa gggcgagatg gcagacggtg aaggccgcct
    11521 gattgactgt aaaaatatcg ttttcttcct gacctccaac cttggctacc aggtgattgt
    11581 cgaacacgcc gatgacccgg aaaccatgca ggaagccctc tatccggtgc tggcggactt
    11641 ctttaaacct gctctgctgg cgcgcatgga agtggtgcct tacctaccgc tgtcgaaaga
    11701 gacgctcgct accattattg ccgggaaact ggcccgcctg gataacgtgc tgcgcagccg
    11761 ctttggcgcg gacgtgatta ttgggccgga agtgacggac gaaatcatga gccgcgtcac
    11821 ccgcgcggaa aacggcgcga ggatgctgga atcggttatc gacggcgaca tgctgccgcc
    11881 gctctcgctg ctgctgttgc agaaaatggc ggccaacacg gcgattgccc ggattcgcct
    11941 gtcggcggca gacggtgcgt tcacggcaga tgtggaagat gcgctgcctg atgatgccgt
    12001 gacaccgcaa acagaggacg aaacggtttt atgagcaggt tgtgcgtttt ccggcagaca
    12061 ggatggttta tcgcgggcct gatggcgggc ttacccgcca gtgccgcgcc cacagaaaca
    12121 tcgtcagtgg ccggtgtggc ggtagctgtc gccacaacga caccgccgga tgcgacagcc
    12181 acgttacagg ccatgcagtc ctgccgccag gagacggcgg cgctggagcg gcttgactgt
    12241 tatgaccgca ttcttgcgcc ggaacaggct ggctttggtg gtgccgcact ggtgaaagct
    12301 cgttaccagg gtgaagcctg ggcccgcgcg acagaacagg aaaaacgccg ccagggcaac
    12361 accacggagc tgctggtaac gcaagtaccg ggtgaacgcc cgacggtggt gataaccacc
    12421 ccggctatcg gccatgttcc gccacgcccg gtgctgatgt tcagttgcgt ggacaatatc
    12481 acccggatgc aggtggcgct gatgcaccct ctggatgtcc atgatatcgc cgtcaccctg
    12541 aatgccgaca gccgggcatt gcgcagccac tggtttgtgc gcgagaacgg caccctgctg
    12601 gagtccagcc gggggttatc cggtatcgat gaaattaagc aactgttcgg cgcaaaaacg
    12661 ctgacggtgg ataccggcac agacaacgcc gccggaaagc tgacctttaa cattgacggg
    12721 ctggcacggg ttattgcgcc gctacgcgac gcctgccact gggcgggaga ataagaatat
    12781 ggcaatggac ttacgtaatc ccgatgtatg gcttgcacac ctgctggaaa acctgccaga
    12841 agataagctg tccgccgcgc ttgatgatgg caatgcagac tgggagtttg tcgacagcga
    12901 aatcgtcaag ctcggttcac tggcgcacag ccagcttgat atcccggaac ttcagcgccg
    12961 gggcctgatg cttctggcct cagaaaccaa agattttcgc ctgctggccc acttgctgcg
    13021 taccctgcaa catgccggtg atattctgct ggcctccagg atgctggcac aatatacaga
    13081 gcactactgg acatgcgccg caccgcagaa catggcccac aaaaaacgct ttgccgccca
    13141 ggtgataaaa cgctttgagt ctgccgttca ggactttgcc gggaatgctg cgacaataca
    13201 gcgcgatgct ttgctgggag agctggcaaa actggcacag tgctggcagg cgcacaacgc
    13261 gtctgagctg gcaaaggcga cagacgattt attttccctg tttcagcgcg cgtttcgtga
    13321 tgtagcgcct gaaatgacat catctgcacg aagcgccgcc attgcgccgc aggccacgac
    13381 cggatatgta ccggatatgt caccgcacac ggttgccgct cagcctcctg ttgcagccac
    13441 tccaatcccg caggtcaccg ttgaaagcca cgacgataaa gcatggcgcg acaccctgct
    13501 aaaggtggcc gccattctct gtgagcgcca gccggagtca ccgcagggtt accgactgcg
    13561 ccgccatgcg ctgtggcaaa ccatcaccag taccccgcag gcagaaagcg atggccggac
    13621 cccactggca gccttccctg tcgatatgat ggatgattac ctggcgcggc tgaacaatgc
    13681 cgacatggcg ctgtggcagc aggtggaaaa aagcctgctg ctggccccgt actggctgga
    13741 cggtcattac ctctcagcac agaccgcgct gcgcctcggt tataaacagg tggcggatgc
    13801 tatccgtgat gaagttaccg attttctggc ccgcctgccc gcactgatca acctgttgtt
    13861 taacgaccgc actccgtttg tgtccgaaca gacgaaacag tggctggcat catccggctc
    13921 agtcaaccag accgtcccgg ttgtacagac cgatgaagaa ttacaggcgg ccaaggcgtg
    13981 cttcgatgaa aacgggctgg aagccgcgct gcggtacctg gaaaacctgc ccgaaggtga
    14041 tccccgccac cagtttcacc gccagttttt cggcgcgcag ttactggaag aggccggtat
    14101 ggttcagctt gcacaacagc agtaccggat gctcttcaga acaggattac acatgatgct
    14161 atccgaatgg gagccttccc tgctgaaagc actggaacaa aaactcacgg cagaacagta
    14221 aaagataaca aggagttact gtgttcaaat ttcccacatc ccgactgttc agcacgttga
    14281 aatctgcgct caggccagcg atgccgcggt ttaaagtttc cgccacctgg ctactgacgc
    14341 tggcatggat ttttctgctg gtgtggatct ggtggcaggg tccaaaatgg acgctctatg
    14401 agcagcactg gctggctccg ctggcaaacc gctggctggc gaccgccgtc tggggactta
    14461 tcgctctggt ctggctcacc tggcgggtga tgaagcgtct gcaaaagctg gaaaaacagc
    14521 agaaacagca gcgggaggaa gaaaaagatc cgttgaccgt ggaactccac cgccagcagc
    14581 aatatctgga tcactggctg ctgcgcctgc gccgccatct ggataaccgc cgttatctgt
    14641 ggcagttgcc gtggtatatg gtcattggtc ctgcgggtag cggcaaaagc acgctgctgc
    14701 gcgagggctt tccgtctgac attgtttaca cgccggaaag catccggggt gtggaatacc
    14761 acccgctgat cacaccgcga gtgggcaacc aggcggtaat tttcgatgtt gacggcgtac
    14821 tgaccactcc cggcggggat gatctgctcc gccgcctgcg cgaacactgg ctgggctggc
    14881 tgatgcaaac gcgcgctcgc cagccgctca acggtcttat cctgacgctc gatcttcccg
    14941 atctgctgac ggcggataaa tcccgccgtg agacactggt acaaaatttg cgccagcaac
    15001 ttcaggagat ccgtcagagc ctgcactgcc gtctgcccgt ttacgtggtg ctgacacggc
    15061 tggatctgct gaacggcttt gccgcgctgt tccattcact ggataaaaaa gaccgcgatg
    15121 cgatcctcgg cgtcacattt acccgccgcg cccatgaaag tgacggctgg cgcagcgaac
    15181 tgggggcttt ctggcagacg tgggtacaac aggtgaacct ggcgctgtcg gatctggtgc
    15241 tcgcacaaac cggtgctgct ccccgcagcg cagtgttcag cttctcccgt cagatgcagg
    15301 gaacaggaga aatcgtcacc gcactgctcg ccgcattgct ggacggtgag aacatggatg
    15361 taatgctgcg tggcgtctgg ctcacatcct cgctacagcg tggccaggtg gatgatattt
    15421 tcacgcagtc cgccgcccgc cagtacggac tgggtaacag ctcgctggca acctggcctc
    15481 tggtggagac gacgccgtat tttactcgcc gcctcttccc ggaagtcctg ctggctgagc
    15541 cgaacctggc gggtgaaaac agcgtctggc tgaacagctc ccggcgcagg ctgaccgcct
    15601 tttccacctg tggcgcggca ctggcggcat tgatggtcgg aagctggcac cattattaca
    15661 atcagaactg gcagtctggc gttaacgtac tggcacaagc taaagccttt atggacgtac
    15721 caccaccgca gggaacggat gaattcggca atctgcaatt gccattgctt aacccggtac
    15781 gcgatgccac cctggcctat ggtgattatc gcgatcacgg ttttctggcg gatatgggat
    15841 tgtaccaggg cgcccgcgta gggccgtatg tggagcaaac ctacattcag cttcttgagc
    15901 agcgttatct cccctcgtta atgaacggcc tgatccggga tctaaacatt gccccgccag
    15961 agagcgaaga aaagctcgct gtgctgcgcg tagtgcgcat gatggaagac aaaagtgggc
    16021 gcaacaacga ggcggtaaaa cagtacatgg cacggcgttg gagcaatgaa tttcacggcc
    16081 agcgcgatat tcaggcgcaa ctgatggtgc atctggacta tgcgctggag cacaccgact
    16141 ggcacgcgca gcgccaaagc agcgacagcg atgctgtaag ccgctggacc ccctataata
    16201 aaccggtcat taatgcgcaa catgaactga gcaagctacc catataccag cgtgtctacc
    16261 agacccttcg caccaaagca ttaagcgtgt tacccgccga tttgaatttg cgcgaccagg
    16321 ttggtcccac cttcgacaac gtgttcgtcg ccggtaatga tgaaaaactg gtgatcccgc
    16381 agtttctcac ccgctatggc ctgcaaagct attttatcaa acaacacgac ggtctcgttg
    16441 agttgaccgc gctggattcg tgggtgctga acctgacgca aagcgtcgcc tacagcgagg
    16501 ccgaccgtga agagatccag cgccatatca ctgaacagta cctcagtgac tataccgcta
    16561 catggcgtgc cggaatggat aatctcaacg tccgtgacta tgagaccatg ccggcgctga
    16621 ccgacgcgct ggagcagatt atcagcggtg atcagccatt cctgcgtgct ctgacggcgc
    16681 tgcacgataa tacacacgcg ctgacgctct ccggcaaact ggacgataag gcgaaggaag
    16741 cggcgataaa tgagatggat taccgcctgt tatcccggct ggggcatgag ttcgcgccgg
    16801 aaaatagcgc actggaggag caaaaggaca aggcgagtac tctacaggcc gtgtatcagc
    16861 aactgaccga gctgcaccgt tacctgctgg cgatccagaa ctcgccagtg tctgggaaat
    16921 cggcgctgaa agcagtacag ctacggctgg atcaaaacag cagcgatcca atcttcgcca
    16981 cccgtcagat ggcaaaaacc ctgcctgcgc ctcttaaccg ctgggtaggt aagctggcgg
    17041 atcaggcctg gcatgtagtg atggtggaag ccgttcgtta catggaagtg gactggcgcg
    17101 acaatgtagt gaaacccttc aacgagcagc ttgccgataa ctatccgttt aatccgcgcg
    17161 ccacacagga tgcctcactg gattcgtttg aacgtttctt taaaccggat ggcattctgg
    17221 acaatttcta caagaacaac ctgcgcctgt tccttgaaaa cgatctgacc tttggcgacg
    17281 acggcagaat gttaatccgt gaagatatcc ggcagcaact ggatactgcg cagaaaatcc
    17341 gcaacatctt cttcagccag cagaacgggc tgggcgcaca gtttgccgtg gaaaccgtat
    17401 cgctttccgg caataagcgg cgcagcgtac ttaacctgga cggccagtta gtggactaca
    17461 gccagggacg caactacacc gcccatctgg tctggccgaa caacatgcgt gaaggcaatg
    17521 aaagccagct gacgctgatt ggcaccagcg gcagagcacc gcgcagtatc gcgttcagtg
    17581 gaccgtgggc gcagttccgc ctgttcggcg cgggccagtt gaccaatgtg accagtgaca
    17641 cctttaacgt gcgctttaac gtggacggcg gcgcgatggt ttaccgggtg catgtggata
    17701 ccgaagataa cccgttcacc ggcggtctgt tcagccagtt ccgtttacca gatacgttgt
    17761 attaagggga tagtgatgag caataacgca ctgacacaaa ctatcgttac cggcagcgat
    17821 ccgcgcgccc tgccggagtt cagcgccatc cgcgaagaaa ttaacaaagc taaccacccg
    17881 tcacagccgg agctgaactg gaaactggtg gaatcgctgg cgctgtcgat ttttaaggcc
    17941 aacggggtgg atttgcatac cgccacctac tatacgctgg cacgtacacg taatcaggga
    18001 ctggcagggt tctgtgaagg cgcggaactg ctggctgcga tgatcatcca cgaatgggat
    18061 aaattctggc cgcagagtgg cccggcgcgg actgagatgc tggactggtt caacacccgc
    18121 accgggaata tcctgcgcca gcaggtttcc ttttccgaaa atgatctttc gttgctctac
    18181 cgcacggaac gggctttgca gcttatctgc gacaaactcc agcaagtgga actgaagcgc
    18241 cagccgcgcg tggaaaatct gctttatttt gtgcagaaca cacgcaagcg gtttgagccg
    18301 cagcccagaa accgtactga tacggcggca cagaccatgg tcagaacgct ggtctatgcg
    18361 cctgaaggca cggcttccgc cacagcggaa accatgcccc ccctacccga cctgccggaa
    18421 atgcgggtgg gggtgcgcgg tgtggcagat aacgccgaca aagcaaaaca gggcgacacg
    18481 ataaaaggtt ttgttgccgg tgccgcctgt actgccgtta tcgcatctgc actctggtgg
    18541 tggcaggcgt atccgatgca gcagcagctt acacaggtca gggataccac acagggagcc
    18601 gccaccgtct ggttggcctc cccggtactg aaagaatatg aacagtacct gcaacagctt
    18661 ctgaatgtcc cgccactaca gccgctggaa accggaatgc agatgatgcg caccgcagac
    18721 accctctggc cggaaagcct gcaacagcag gaagccagca ggatgtggag caacacgctg
    18781 agaaaccggg cgcaagccag tccgcagatg aagggctggc aacaagcgcg gcagaatttg
    18841 cgggattttg cagatttgat gatgaagaag gagacggaga aacaaggttt caccctatcg
    18901 tatatcaaaa cggtgacctg gcaggccgaa cggttactga atcaagaaac accactggag
    18961 tacctgctga cgcagtacca ggagacccgt acacagaagc aggacacaca ggcgttggaa
    19021 aaagagatca acgaacggct ggacgggctg ttaagccgct ggctgctgct gaaaaatacc
    19081 ggacaggata tggcaacgga taacagaacc gaacctgtcc atccaactca ttgagattaa
    19141 ggagaataac aatggcaaat cccgtctatc tgacactgaa cggagaactt caggggctga
    19201 tttcatccgg atgctcgtca atgccgtcca tcggtaacaa agcccagatt gcacatcagg
    19261 atcagatcat ggtgacgtca ctgtcccatg gtctgagccg tgcgcagaat gtcaaccatc
    19321 aggagttgac catcacgaaa ccggtggaca aatcctcccc tctgctgggc aaggccattt
    19381 cagagaatga atgtctgacc tgtgattttg tattctaccg caccaaccgc tttggaatta
    19441 atgaaccgta ctacaagctg aaactggcta acgcccggct ctcaaacatc ggccttaccg
    19501 ttccccatac cattaacgac agtcctgggc aggcggaaga gtctgtgtca tttacctatg
    19561 agagcatcaa ttgggagcac agcgtcgccg gaaccagtgc atacagcctt tggagtgaac
    19621 ggattttcta agttaccagg gagtctttca tctgcattat tgttatattt taaaaaggca
    19681 atttaacatt ataggataaa atgacaaagt acggttaatc acgaataaaa attcttaatt
    19741 aaccgtacct gctgttttat agatgtatat gatgtatttt tgacagttca accga
//
LOCUS       DAZGJT010000087        19751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZGJT010000087 DAZGJT010000000
VERSION     DAZGJT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.087"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..671
                     /gene="yfaZ"
                     /locus_tag="ACHKFK_003482"
     CDS             129..671
                     /gene="yfaZ"
                     /locus_tag="ACHKFK_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO9053747.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDAAGVGLGLNLPLGPLMATVGGKGVYTNPNDGDEGYAAAVGGG
                     LQWQIGDSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMRPVSIEAGYRYLNLAGK
                     NGNRDNAIADGPYVGVNASF"
     gene            771..1973
                     /gene="yfaY"
                     /locus_tag="ACHKFK_003483"
     CDS             771..1973
                     /gene="yfaY"
                     /locus_tag="ACHKFK_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO9053748.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAIQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEEQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQSRQFSLTLSEQF
                     TGGLLALQLSRADAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGLEDEH
                     LNFALATPDGTFALRVHFSATRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            2193..2975
                     /gene="yfaX"
                     /locus_tag="ACHKFK_003484"
     CDS             2193..2975
                     /gene="yfaX"
                     /locus_tag="ACHKFK_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HFO9053749.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPEAVQKSIIEEL
                     VWEQMTPTTITHPQRLYEELARIRRQGWSYDNGEDYADVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            2990..4195
                     /gene="rhmD"
                     /locus_tag="ACHKFK_003485"
     CDS             2990..4195
                     /gene="rhmD"
                     /locus_tag="ACHKFK_003485"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050032 - L-rhamnonate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HFO9053750.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLIVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYAIKLAHACAPYNLKWIEECLPPQQYEGYRELKHNAPAGMMVTSGE
                     HHGTLQSFRTLSENGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4252..5541
                     /gene="yfaV"
                     /locus_tag="ACHKFK_003486"
     CDS             4252..5541
                     /gene="yfaV"
                     /locus_tag="ACHKFK_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9053751.1"
                     /translation="MSTALLDAVVKKNRARLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVLAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFINLFGAVGGFIAPILRVKAETLFS
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            5559..6362
                     /gene="yfaU"
                     /locus_tag="ACHKFK_003487"
     CDS             5559..6362
                     /gene="yfaU"
                     /locus_tag="ACHKFK_003487"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HFO9053752.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTAYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASHPVIRPVEGSKPLIKQVLDIGAQTLLIPMVDTA
                     DQARQVVSATRYPPYGERGVGASVARAARWGRIENYMVQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRVKGSY"
     gene            complement(6403..6600)
                     /locus_tag="ACHKFK_003488"
                     /pseudo
     CDS             complement(6403..6600)
                     /locus_tag="ACHKFK_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6601..7560)
                     /gene="rpnE"
                     /locus_tag="ACHKFK_003489"
     CDS             complement(6601..7560)
                     /gene="rpnE"
                     /locus_tag="ACHKFK_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO9053753.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKIEGWQEGKLEGLQ
                     KGKVEGMHEQAIKIALRMLEQGFEREIVLATTQLTDADIPNCY"
     gene            complement(7753..8943)
                     /gene="glpC"
                     /locus_tag="ACHKFK_003490"
     CDS             complement(7753..8943)
                     /gene="glpC"
                     /locus_tag="ACHKFK_003490"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO9053754.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSVAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSLCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(8940..10199)
                     /gene="glpB"
                     /locus_tag="ACHKFK_003491"
     CDS             complement(8940..10199)
                     /gene="glpB"
                     /locus_tag="ACHKFK_003491"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO9053755.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTEIHSGLESLRQQAPAHPYTLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKTCVVGISGLMDFQAHLAAASLR
                     ELDLKVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWHWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERDGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQLFGVTTDEALRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHVAQQIAQRAGGQQ"
     gene            complement(10189..11817)
                     /gene="glpA"
                     /locus_tag="ACHKFK_003492"
     CDS             complement(10189..11817)
                     /gene="glpA"
                     /locus_tag="ACHKFK_003492"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO9053756.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEVIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGEIQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEATLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12090..13448
                     /gene="glpT"
                     /locus_tag="ACHKFK_003493"
     CDS             12090..13448
                     /gene="glpT"
                     /locus_tag="ACHKFK_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO9053757.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13453..14529
                     /gene="glpQ"
                     /locus_tag="ACHKFK_003494"
     CDS             13453..14529
                     /gene="glpQ"
                     /locus_tag="ACHKFK_003494"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9053758.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNVLEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            14732..15382
                     /gene="inaA"
                     /locus_tag="ACHKFK_003495"
     CDS             14732..15382
                     /gene="inaA"
                     /locus_tag="ACHKFK_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO9053759.1"
                     /translation="MAVSAKHDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(15436..15690)
                     /gene="yfaE"
                     /locus_tag="ACHKFK_003496"
     CDS             complement(15436..15690)
                     /gene="yfaE"
                     /locus_tag="ACHKFK_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO9053760.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(15690..16820)
                     /gene="nrdB"
                     /locus_tag="ACHKFK_003497"
     CDS             complement(15690..16820)
                     /gene="nrdB"
                     /locus_tag="ACHKFK_003497"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO9053761.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(17010..19295)
                     /gene="nrdA"
                     /locus_tag="ACHKFK_003498"
     CDS             complement(17010..19295)
                     /gene="nrdA"
                     /locus_tag="ACHKFK_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO9053762.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4554 a         4879 c         5360 g         4958 t
ORIGIN      
        1 atcaccaaga agtatttagt tacaataagt gactagatcc cttttttcgc tcattgaatc
       61 ctctgcgcca ggcagtacaa tcccgcagct ttgggaattt tgattaattg cttgtgggaa
      121 agaggataat gaaaaaaatt gcgctagcag gtctggccgg aatgctgttg gtttctgcat
      181 cggtcaatgc aatgagcatc agcggccagg cgggtaaaga atacaccaat attggtgtcg
      241 gttttgggac tgaatcgacg ggcctggcgt taagcggtaa ctggacacat aacgacgacg
      301 acggtgatgc cgcgggcgtg gggctggggt tgaatctgcc tctcgggcca ttaatggcga
      361 ccgttggcgg aaaaggcgtt tacaccaacc cgaacgatgg tgatgaaggt tatgcagcgg
      421 cagttggcgg tggtttgcag tggcaaattg gtgacagctt ccgcttgttt ggtgagtatt
      481 actactctcc ggattcgctc tccagcggaa ttaaaagtta tgaagaagcg aatgctggcg
      541 cgcgttacac cattatgcgt ccagtcagca ttgaggctgg ctaccgttac ttgaacctgg
      601 caggtaaaaa tggcaatcgt gataacgcca ttgctgatgg tccgtatgtt ggggtaaatg
      661 ccagcttctg acgccttccg gcgcggcgtt ctgtcgcgcc attcagtttc ttactacgcc
      721 ctgtctccct ttgcgttata gtgattcgat aacaaaagca ggagataacc atgttaaaag
      781 tggaaatgtt atccaccggg gatgaagtgt tacacgggca aatcgttgac actaacgctg
      841 cctggctggc cgattttttc tttcatcagg ggctgccatt atctcgccgc aatacggtgg
      901 gggataacct tgatgactta gtcaccattc ttcgcgaacg tagtcagcac gccgatgtgt
      961 tgatcgttaa cggcgggctg ggaccgacca gtgatgattt aagcgcactc gccgctgcga
     1021 cagcaaaagg tgaaggcctg gtgctgcatg aagcctggct caaagagatg gaacgctatt
     1081 tccatgaacg gggacgagta atggcaccga gcaaccgtaa acaagcggag ctgcctgcca
     1141 gtgctgaatt tatcaataac ccggtaggca ctgcctgtgg ttttgccata cagcttaatc
     1201 gttgcctgat gttctttacc cccggcgtac cgtcagaatt taaggtgatg gtcgagcacg
     1261 aaatcctgcc gcgcctgcgc gagcgttttt ctttaccaca gccgccggtt tgcctgcgtt
     1321 tgactacctt tggtcgttcg gaaagcgatc tggcacaaag cctggacact ctacaactgc
     1381 cgccgggcgt aacaatgggc tatcgctcct caatgccgat tatcgaactg aaactcaccg
     1441 gaccggcaag cgaggaacag gcgatggaaa aactgtggct ggacgttaaa cgtgttgccg
     1501 gacagagcgt gattttcgaa ggcaccgaag gactgcccgc gcagatcagt cgcgaattgc
     1561 agagccgcca gttcagcctg acgctgagcg agcaattcac cggtggttta ttggctttgc
     1621 aactttctcg cgcagatgct ccattgctgg cgtgtgaagt ggttccttca caggaggaaa
     1681 ccctggcgca aactgcgcac tggattacag aacggcgggc gaaccatttt gctgggttgg
     1741 cactggctgt ttctggtctg gaagatgagc atctcaactt tgcactagcc acgccagacg
     1801 gcactttcgc tttgcgtgta catttcagtg ccacacgcta cagtctggct attcgccagg
     1861 aagtctgcgc aatgatggcg cttaatatgc tacgccgttg gttaaacggc caggacatcg
     1921 ccagcgagca tggctggatt gaggttgttg agtccatgac cttatctgtc tgattccgcc
     1981 actctgtgcc ggtttactgc accggcacat tccccctccg gcaaaatttg tccaacctgt
     2041 tgtactcaat aagaaaaaat ccgtgcgctg attcacaaat ttgtgcgtaa tttctccagt
     2101 ccgctggacg agaaacggga tttgatgcgc ttcacaattt tactccccct gagcaacgag
     2161 actgattcca gtatctaaga ctggagtcca gtatgttgga atcaagcaaa gttcccgccc
     2221 tgacacgggc tattgatatt ctcaatttga ttgcccggat tggcccctgt agtgctgcga
     2281 cgatcattga cacattgggg atccctaaaa gcacagccta tttgctgctt aatgaactca
     2341 ggcgtcagcg ttttcttagc cttgatcacc aggaaaattt ttgtttgtgg accaggctgg
     2401 tggagctttc cggtcatgcg ttaagcaaga tggatctccg ggaactggct cgaccgcgtt
     2461 tgacgcaact gatggatacc accgggctgt tatgtcacct ggggattatt gataacggca
     2521 gcgcttatta cattttgaaa gtggagtcat cagccactat cagcgtgcgt tcccatgaag
     2581 gaaaaagcct ttcgctttat cgctccggaa taggtaaatg cctgcttgcc tggcaacctg
     2641 aagctgtaca gaaaagtatt atcgaagagc tggtctggga gcagatgaca cccaccacca
     2701 ttacccatcc acaacggctc tacgaggaac tggcgcgtat tcgccgtcag ggctggagct
     2761 atgacaacgg cgaagattat gccgatgtcc gttgtgtcgc tgcgccggtg tttaacgcca
     2821 ataacgagct taccgctgcg atttcggtgg tcggtacccg cttacaaatc aatgaagaat
     2881 accgtgatta tctggcgggc aaggccattg cctgcgccag ggatatttca cgtttgttgg
     2941 gatggaaaag tccctttgac ttacaggcct cataacaatg gagaacatca tgaccctacc
     3001 aaaaatcaaa caggttcgcg cctggtttac tggcggtgcg acagcagaaa aaggcgctgg
     3061 cggcggtgat tatcacgacc agggggcgaa tcactggatt gatgatcata ttgccacccc
     3121 gatgagtaaa taccgcgatt acgagcagtc acgccagtca tttggcatta acgttcttgg
     3181 cacgttgatt gttgaagttg aagcagaaaa cggccagacc ggattcgctg tttcgacagc
     3241 cggtgaaatg ggctgtttta ttgtcgaaaa acatcttaac cgtttcattg agggtaaatg
     3301 cgtcagtgat atcaaactga tccacgatca aatgctcaat gccaccctgt attactccgg
     3361 ctctggtggc ctggtgatga atacgatttc ctgtgtcgat ctggctctgt gggatctgtt
     3421 cggcaaagtg gtcgggcttc cggtttataa acttttaggt ggtgcagttc gtgatgaaat
     3481 tcagttctac gccacaggtg cgcgtccgga tctggcaaaa gagatgggct ttatcggtgg
     3541 caaaatgccg acgcactggg ggccacatga tggtgatgcg gggatccgca aagatgccgc
     3601 tatggtcgcg gatatgcgtg aaaaatgcgg tgaggatttc tggttaatgc tcgactgctg
     3661 gatgagtcag gacgtgaact atgccatcaa actggcccac gcttgcgcgc cctataacct
     3721 gaaatggatc gaagagtgcc tgccgccaca gcagtatgaa ggttatcgcg aactgaaaca
     3781 caacgcacca gcggggatga tggtcaccag cggtgagcac cacggcacac tgcaatcttt
     3841 ccgtacgctt tcagaaaacg gtatcgacat tatgcagccg gatgttggct ggtgcggtgg
     3901 cttaaccacg ctggtggaaa ttgccgcaat cgccaaatcc agggggcaac tggtggtgcc
     3961 acacggttcg tctgtttact cccaccatgc ggtgatcacc ttcaccaata cgccattcag
     4021 cgaattcctg atgaccagcc cggattgttc aacgatgcgt ccgcaatttg atccgattct
     4081 gcttaatgag ccagtaccgg tgaatggtcg tattcataaa tcagtgcttg ataaacccgg
     4141 tttcggcgtc gaactcaatc gtgactgcaa tttgaaacgc ccctacagcc actaatcact
     4201 ccgggcgttg ctgtgacaac ggtaacgccc acatattcta aggagctttt tatgagcacc
     4261 gctttgcttg acgccgtggt gaagaaaaac cgcgcgcgtt taattccgtt tatgttggcg
     4321 ctgtatgtgc tggcgtttct cgaccgttcg aatattggtt ttgccaaaca gacctaccag
     4381 attgataccg ggctgagtaa tgaagcttat gcgctgggag caggcatttt ctttgtggta
     4441 tacgcgtttc tgggggttcc ggcgaatctt ttgatgcgca aactgggggc cagaacctgg
     4501 attggtacga caacactgct gtggggattt ctttcggctg ccatggcatg ggccgatact
     4561 gaagcgaaat ttctgattgt tcgcactctg cttggtgctg cggaggccgg gtttttccct
     4621 ggtatgattt atctcacctc gcaatggttt ccgcagcgta atcgcgccag cattatgggg
     4681 ctgttctata tgggcgcacc gctggcgtta acactgggat caccgctttc cggcgcgctg
     4741 ctggagatgc atggatttat ggggcatccc ggctggttct ggatgtttgt tattgaagga
     4801 ttgttggcag tcggcgctgg ggtattcaca ttcttttggc ttgatgacac accggagcag
     4861 gcacgttttc tgagtaaaca agaaaaaacg ttgcttatca atcaactggc aagtgaagaa
     4921 caacagaaag tgacttctcg attgagcgat gcgctgcgta atggccgagt ctggcaactg
     4981 gcgattatct acctgaccat tcaggtagcg gtttacggat taattttctt cctgccgacc
     5041 caggttgcgg cattgctggg aacaaaagtg ggctttacgg cgtcggtggt caccgccatt
     5101 ccgtgggttg cggccttgtt tgggacctgg cttattccgc gctactccga taaaaccggc
     5161 gaacggcgta atgtcgcagc gctgacatta ctggcggcgg gcattggtat tggtctgtcc
     5221 gggctgcttt ctccagtact ggcgatcgta gcactgtgtg ttgcagccat cgggtttatt
     5281 gccgtgcaac cggtgttctg gacgatgccg acacagcttc tttccggtac ggcgctggct
     5341 gcggggattg gttttataaa cctgtttggt gcagtgggcg ggtttattgc cccgatcctg
     5401 cgcgtgaaag cagaaacgtt attttccagc gatgcggcgg gattactgac gctggcagcg
     5461 gtggcggtca tcggttcgct gattattttc actctgcgtg taaatcgcac tgttgcgcag
     5521 accgacgtgg cacatcatta aatagtttaa ggaacacgat gaacgcatta ttaaccaatc
     5581 cctttaaaga acgtttacgc aagggcgaag tgcaaattgg tctgtggtta agctcaacga
     5641 ctgcctatat ggcagaaatt gccgccactt ccggttatga ctggttgctg attgacgggg
     5701 agcatgcgcc aaacaccatt caggatcttt atcaccagct acaggcggta gcgccctatg
     5761 ccagccaccc cgtgatccgt ccggtggaag gcagtaagcc gctgattaaa caagtcctgg
     5821 atattggcgc gcaaactcta ctgatcccga tggtcgatac tgccgatcag gcacgtcagg
     5881 tggtgtctgc cacgcgctat cctccctacg gtgagcgtgg tgttggggcc agtgtggcac
     5941 gggctgcacg ctggggacgc attgagaatt acatggtgca agtcaacgat tcgctttgtc
     6001 tgttggtgca ggtggaaagt aaaacggcac tggataacct ggacgaaatc ctcgatgttg
     6061 aagggattga tggcgtgttt attggacctg cggatctttc tgcgtcgttg ggctacccgg
     6121 ataacgccgg gcacccggaa gtgcagcgaa ttattgaaac cagcattcgg cggatccgtg
     6181 ctgcgggtaa agctgctggt tttctggctg tggctcctga tatggcgcag caatgcctgg
     6241 cgtggggagc gaactttgtc gctgttggcg ttgacacgat gctctacagc gatgccctgg
     6301 atcaacgact ggcgatgttt aaatcaggca aaaatgggcc acgcgtaaaa ggtagttatt
     6361 gatatcaaag gcccatgggg atcggctgtg ggcctgtgtt aattagtggt tatttgctgc
     6421 cagatcggct tcgcttagct gagtggccgc gagcacctgg tcacgatcaa tgccctgttc
     6481 cagcatgcgc aaggcaattt taatggcttg ttcatgcatg ccttctaatt taccctcctg
     6541 ccagccaatt tgatgtcctt cctgccgtaa ccgttcagca atagtcatta atctctcctt
     6601 ttaataacag ttcggaatat cagcatcagt gagttgggtt gtcgccagca caatctcacg
     6661 ttcaaagccc tgttccagca tgcgcaaggc aattttaatg gcttgttcat gcataccttc
     6721 tactttgcct ttctgcaaac cctccaattt cccctcctgc caaccttcaa ttttaccttc
     6781 ctgccagcca atttgatgcc cttcctgccg cagccgttca gcaatagtca ttaatctctc
     6841 cttttgtagt ggtgaacgtt cggcaatctc ctgaataaaa cgggtgaaac gggaggtatc
     6901 gccgcattgc agcagataat taaacagtgt ttgtagctga ctgtcattag tgaagcctct
     6961 aaccaaaagc gtggtgatcc tgtcgaccat accgattaaa tcgcggtcgc gaatatgctt
     7021 ttgaatcagt tccagcagag ctatacgccg atgttgcatg atctcatcgt caggtacgat
     7081 ggtgatatcc accaacggaa aagcttcggt gtacaactgc cgggcaagtt gcggatcatc
     7141 aaactcatcc agccagttaa gtgagtatgg gtagggcgag gtttcgccat gataaaacag
     7201 caacggcacc accagcggga ctctgtcata gcctttgtcc agatggcgct gcatggcggc
     7261 ggtggcatag cgcattagtc gaaaagccat attcttttct gcagaacttt gatgttcaat
     7321 cacgcagtag atatagccgt caccgtcgct ggtttccacc gaccacaaaa catccgagta
     7381 gtaagcgcgt aaactttttt caataaaact ggtgggttcc aggcgtaagg tttgcaggtt
     7441 gcaaagcttg cgcagtggtt ctggtaaatg tatttcgaga aaatcccgtg cggtttcggg
     7501 tgtgaacata aaggttttaa ataccgcatc atgcggggag gaggttgttg attcggtcat
     7561 ggttgtccgt cacctgatat ttatgatgac gcgacattac ggttatccca ggcctttgac
     7621 atctgccagt ttcagccttt gcgagacgtt ttcagaggaa ttttcactgc gttgcaacgt
     7681 atacattgag cgcggaatcg tgtacgcaaa aatgcccggt aaccacatgc ttaccgggca
     7741 tcagaaagga gtttaagcca gcgcctgggc cagcagcgta atcggatgtt cgcagcgaag
     7801 gcttgtcgac atctcaatct gccatttaca ggtttcgcag tcggtgacca ccagatctgc
     7861 gccgctttct tctatctggc ggaacagtgg tgcgccgatg gcttgtgagg tggggtagtt
     7921 ctcttttttg aaaccgtaag ttcccgcaat accgcagcac agggaatcca gcaccgttaa
     7981 ctcaagcccc gggattttac gcaacagctc cagggtgtag agcgtccagc ccattttttc
     8041 catatggcac ggagtgtgat aaaccacttt tagcggcagc ggtttcagcg gtaacgtttt
     8101 gccttcgtcc agcttgcgcc acagccagcg ggtcgccagt tcgatgtgat cgcgtaagcc
     8161 tttgttgtcg acattcagca cttccgggta ttcgtcgcgc agggcaaatg tacacgttga
     8221 ggaggtggca atcaccggaa tgccttttac tcccacagct tcgcggatcg actctacatt
     8281 cgtaattgcc tgtttgcgtg ctttatcggt aaaaccgttg gcgatcagcg gtacgccgca
     8341 gcatttttct ttgctgagca gttgtacgcc ggtacccatc gcgttgagca ctttaattaa
     8401 atctttgcct agctgcggat ggttgtagtt aacgaagcag ccgtgaaaga aagcgacctg
     8461 gtctttatac tgcgcttgct gagccgccac gctgcgatac cagcgacgga acgtaccgaa
     8521 ggagtatttc ggtagcgtgc gacggtggtc tatttttaac gccgcatcaa gcaactgccg
     8581 caccggtttc agcgaggtag cggtgttgac gatgggtgcg aacggcgtgg aaacgctacc
     8641 catcaggtcg gtatgactca acacaaaatt acgcagcgac gggcgtgtgg tgtcatattt
     8701 cgcccgcgcg cgctggataa tatcgccaat cttcacatcg gacgggcagg cgacttcaca
     8761 acgtttgcag ttgatgcaat atttcagcgc ctcgtcatac agtgcgccat cttttaaacg
     8821 cagacgctcg ccatccggcc cagcttgttt tggccccgga taacccggat tcacccggct
     8881 caccgggcag gcggtggtgc agacggtgca cttaatgcag ttttcgaagc tggtgtcatt
     8941 cattgttggc ctcctgcgcg ttgggcaatc tgttgagcga catgtaaagc actgacggca
     9001 caaacaccgc cgccgcatcc ctgggcgatg ggatcaaatc cgcccagcac cgaaccgatg
     9061 gcaaacaggt tctcaatggt ttgccccgcc tgcgacgggc gtagcgcctc atcagtggtt
     9121 acaccgaaca gctgccacgg ctgcggcgca aaaaaatcgc ccttatacca ttcaccccgc
     9181 gtagcggttt gtagcacatc aaggccgaga atcggctcgc gaatgccgtc acgttctgcc
     9241 accagtcctc cgctaaagaa actgccgctc gcaagcaccg caaatcgtgg gcgcagcggg
     9301 atatcggcgt gattgcgcgt ccagatttca ttcactacac catttttaca ggtcactttt
     9361 ttcacttcat cgcccggcat ccacacgcca ccctggcgca caaactggcg ctgtaactgg
     9421 ttttgcagac gaatgcccag cacggaaggc ggcagcgttg gcaaaagcat caatgaacaa
     9481 ggtagttttt cattcaacca atgccacagt ttgtcatcgg ccaggccgaa gcaggcgggc
     9541 atcaggatca tttcgcaggt attggcgacc ggaataagtg catcaagtaa caacggccag
     9601 ttttcttcat tatcaaggaa gcgggcaata ttcaccgccc ggaactcggt ggcgttattg
     9661 cgcagcacat ccagttccgg cagttcaatt tcagcggttt caactttaag atccaattcg
     9721 cgcaatgaag ctgccgcaag atgcgcctga aaatccatca ggccgctaat tcccactaca
     9781 catgttttct tcgcgggcag cggccagaca gggacttctg gcgaacttag ccaggttgag
     9841 cgcagagtgc ctagcggtgt aatccgctgg tgagccagct ctacgctgcc ctgcaattgc
     9901 gcaccgcttt cagcgattaa tgcctgcgcc tggcaagcga gatcgagcac gcgttgtggc
     9961 ccgagaagag tgtaaggatg ggcaggtgcc tgctgacgca aagattccag tccactgtga
    10021 atttctgtca ccggttgacc atccggcaga tggctcagca aatccagcga tccagatgag
    10081 aaatgcagcg cgctttgacc acgagtgaca atggcacagc gcaggccgtg tttttgcagt
    10141 tgcaggccac agagtaatcc ggcgaggccg ccgcccataa tgacagtatc aaagcgcatc
    10201 tttctgctcc ttctccagac cacacaatcc ctgataaacc cagcgggtaa attcgctttc
    10261 gcgcagtgca tctccccagg cgatgggttg cacgcctttc cagcgttcgt ttaggaaggt
    10321 ggaaagttgc tcgatagatt gcgcggacgt cgtgacgtta aaacgttgca gcagtccggc
    10381 agcgcggcag gcgcagagtt cgccctggca ggtgcccatc cccacacggg tacgacggcg
    10441 taaatccagc aggctattaa cgtttaaatt ttctaccgcg tactgcactt cacccgcagt
    10501 taccgcttcg cactcacata ccaggctacg gtgcagacgg ccttcgctca gccaggcagg
    10561 cgtgcgatcg ccatgacgat aaaccgcaga accgcgcagc ggggcaggca gggagatgac
    10621 tttacgcaag gtagcttcag cgggttcctg tgaaccagga agtgccagat cggcggtcgt
    10681 acaggggcgc gtgttgccca gtttgcggca tacggcgtcg gtagcccatt ccgccattag
    10741 ccgataggtc atcagtttgc caccggtgat ggtgataaac ccgtccagac catcgcgttc
    10801 agcatggtcg agcagcacga tgccacggct gacgttacgt ccgctcggat cgtcatcgct
    10861 ggcaaccagc gggcgtacgc cagagtaggc ccgcaaaatg cgcgttttcg ccatcacggg
    10921 ggccagtttt tccccttcac gcagcagaat atcaacttct tctgccgtca ctcgattatc
    10981 gtcaatctcg ttgtaatcaa tacgtaaaga ggtggtgcca atcagcgaaa tggtatcgcc
    11041 aggcaccaga atatcggcgt cggaaggttt gcggcagcgg ttgatcacat gctgattaat
    11101 gcggtgatcc attatcagca gcgatccttt cgccgggaac atgcgaatgc gcagatcggc
    11161 atattcggca atgtgttgcc cccagatccc ggcggcatta actacgacag gcgcatgaag
    11221 tgcctgaatt tcgccggtga gatggttacg tacacgaaca ccgcacaccg tcgcgccttc
    11281 acgaatcagc cccgtgactt catgagcggt aaggataacg gcaccgtgtt ctttggcatc
    11341 cagcatgttt gctgctgtca gacgaaatgg atcaacggtg ccatccggaa ctttcaccgc
    11401 gccaatcagt gccgggttaa cggcaggttc gataatgcgc gcttgctgcg gatcaatgac
    11461 ttctgcgctg atccctgctt cttcgcaggc gcggataaaa gtggcctgga aggagaggtc
    11521 atcttccggc agggtgataa acaggccgtt ggttggttct acgcagtgac gtgcaatgcg
    11581 tttcaggatc tggttttcac taatacattc gcgggccgat tccgcatcgg ttaccgcata
    11641 gcgcgcaccg ctgtgcagca ggccgtggtt acgcccggtg gcaccggttg cgatgtcgtg
    11701 gcgctcaacc aaaatcacgc gcagcccgcg cagggcacag tcgcgggcaa tcccggctcc
    11761 cgttgcgcca ccgccaatga taatcacgtc acttgattgc gagtcgcgag ttttcattgt
    11821 tatccctctg aagttcgttt tttaccattt agccatagta aaaacatgaa ttgtttgatt
    11881 tcgcgcatat tcgctcataa ttcgaaagtg aaacgtgatt tcatgcgtca ttctgaacat
    11941 ttcaaccatc gtatttaata atgtgtgcgg taattcacat ttaatttatg aatgttttct
    12001 taacatcgcg gcaactcaag aaacggcagg ttctcccact gaatcaggct gttaatcata
    12061 aataagacca cgggccacgg aggctatcaa tgttgagtat ttttaaacca gcgccacaca
    12121 aagcgcgctt acctgccgcg gagatcgatc cgacttatcg tcgattgcgc tggcaaattt
    12181 tcctggggat attctttggc tatgctgctt actatttggt tcgtaagaac tttgcgctcg
    12241 ctatgcctta tctggttgag cagggattct cacgcggtga tttaggtttt gccctttcgg
    12301 ggatctcgat tgcttatgga ttttcgaaat tcatcatggg ttcggtatcg gatcgctcga
    12361 atccgcgcgt tttcctgccc gcaggtttga ttctggcggc ggcagtgatg ttgtttatgg
    12421 gctttgtgcc atgggcgacg tcgagcattg cggtgatgtt tgtactgttg ttcctctgcg
    12481 gttggttcca ggggatgggg tggccgccgt gtggtcgtac tatggtgcac tggtggtcgc
    12541 agaaagaacg tggcggtatt gtgtcagtgt ggaactgtgc gcacaacgtc ggtggtggta
    12601 ttccgccgct gctgttcctg ctggggatgg cctggttcaa tgactggcac gcggcgctct
    12661 atatgccagc tttctgtgcc attctggtgg cactgttcgc ctttgcgatg atgcgcgata
    12721 ccccgcaatc ctgtggcctg ccgccgatcg aagagtacaa aaatgattat ccggacgact
    12781 ataacgaaaa agcggaacag gagctgaccg cgaagcagat cttcatgcag tacgtgctgc
    12841 cgaacaaact gctgtggtac atcgccattg ccaacgtgtt cgtttatctg ctgcgttacg
    12901 gcatcctcga ctggtcaccg acttatctga aagaggttaa gcatttcgcg ctggataaat
    12961 cctcctgggc ctacttcctt tatgaatatg caggtattcc gggcaccctg ctgtgcggct
    13021 ggatgtcgga taaagtcttt cgtggtaacc gtggggcaac cggcgtcttc tttatgacac
    13081 tggtgaccat cgcgactatc gtttactgga tgaacccggc aggtaaccca accgtcgata
    13141 tgatttgtat gattgttatc ggcttcctga tctatggccc tgtgatgctg atcggtctac
    13201 atgcgctgga actggcaccg aaaaaagcgg caggtacggc agcgggtttt accgggctgt
    13261 ttggttacct cggcggttcg gtggcggcga gcgcgattgt tggctacacc gtggacttct
    13321 tcggctggga tggcggcttt atggtaatga ttggcggcag cattctggcg gttatcttgt
    13381 tgattgttgt gatgattggc gaaaaacgtc gccatgaaca attactgcaa aaacgcaacg
    13441 gaggctaatg gcatgaaatt gacgctgaaa aaccttagca tggcgatcat gatgagcact
    13501 atagtcatgg gaagcagtgc aatggcggcg gacagcaacg aaaaaatagt catcgcccat
    13561 cgcggtgcca gtggatattt gccggagcat acgctgccag caaaagcgat ggcgtatgcg
    13621 cagggagcgg attatctgga acaggatttg gtgatgacca aagacgacca tctggttgtt
    13681 ctgcatgacc attatctcga tcgtgttact gatgttgccg atcgtttccc ggatcgggcg
    13741 cgcaaagacg gtcgttacta cgcgatagat ttcacgctgg atgaaattaa gtcgctgaaa
    13801 tttaccgaag gtttcgatat tgaaaacggt aaaaaagtgc agacttatcc ggggcgtttc
    13861 ccaatgggta aatctgactt ccgggtgcac acctttgaag aagagattga atttgttcag
    13921 gggttaaacc actctaccgg gaaaaatatc ggtatctatc cagaaattaa agcgccgtgg
    13981 ttccatcatc aggaagggaa ggatattgcg gcaaaaacgc tggaagtgct gaagaaatat
    14041 ggttacaccg gtaaggacga caaagtttat ttgcaatgtt ttgatgctga tgagctgaag
    14101 cgtattaaga atgtgctgga acccaaaatg ggtatggatc tcaatctggt acagttgatt
    14161 gcctataccg actggaatga aacgcagcag aaacagccgg acggaagctg ggttaattac
    14221 aattacgact ggatgtttaa gccgggtgcc atgaaacagg tggcggaata tgctgatggt
    14281 attggtccgg attaccatat gttgattgag gagacatcgc agccgggtaa tatcaaactc
    14341 actggcatgg tgcaagatgc tcagcagaat aaactggtag tgcatcctta taccgtgcgg
    14401 tcagataaac tgcctgaata cactactgat gtgaatcagt tatatgatgc tctgtataac
    14461 aaagcgggtg taaatgggct atttactgat ttccctgata aggcagtaaa attccttaat
    14521 aaagagtaaa ttaactaccg gatggcactt atgtgctatc cgataccact cttcattaaa
    14581 gtctgtgggt tatccttaat tgcaattatt tagagcgaga tgacttgttc ccggcataag
    14641 atactcgtca atacaacacg tattattaag attttcctca gctaaacatc tatagtcatt
    14701 gccgttaata aaaaacgaca ggcgataggg tatggcagtt tcagcaaagc acgacgaatt
    14761 taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc gccgtaatgg
    14821 tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa agcgcatgac
    14881 gcatcatctg tttcattccg tacgttatcc gttcggccga ccaacgattg tccgtgaggt
    14941 tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg tttttggtga
    15001 agcggtgaaa attgagggtg aatggcgcgc gttgctggtg actgaagata tggcggggtt
    15061 catcagcatt gctgactggt atgcccggca tgcagtatcg ccttattctg acgaagtacg
    15121 gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagcg ttaatcgtca
    15181 gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg cagaagctgg
    15241 atttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgt gataaagcga ttaatcatga
    15301 cttccgccag ctggaaaaat atctggagcc gatccccaaa gcggactggg agcaggtgaa
    15361 agcatattac tacgctatgt aatcgttgta ggtcagataa gacgtataag cgtcgtatct
    15421 ggcgttaaaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc aacaacaggg
    15481 caaaatttcc cccggctgaa taaaagctaa cggttcggca atccagtcca cctggcctgc
    15541 gaccagtcgg gtgcgacagg agccgcagta accttcgcgg cactggtact caaccgccac
    15601 attgtgggat tccagcgccg ccagaagaga agggtgttca tcctggcaca gcagttgtgt
    15661 gccagtaatg cgcagggtaa cgcgggccat cagagctgga agttactcaa atcgtcggtg
    15721 tccacttccg cgtcaatctg cccgaccaga taagaactga cttccacttc ctgcggagca
    15781 acctgcacgt tatcagacac cagccaggtg ttgatccacg ggatcgggtt ggagcgcgtc
    15841 tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta ttcaacgtac
    15901 tggcagagga tgtctttatt cagaccaatc atcgaaccat cacggaacag ataatccgcc
    15961 cagtctttct cttgctgtgc cgcctggaca aacaggtcat agcactcctg cttacattct
    16021 tcggcaattt ccgccatctc cggatcatcc gcgccgctgc gcagcagatt cagcatatgc
    16081 tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat tttggcgtta
    16141 ccttccatca gctcacgttc agcgaatgcg aaagagcagg caaagctgac gtagaaacgg
    16201 atcgcttcca gcgcgttaac gctcatcagg cagagataca gttttttctt cagctcgcgc
    16261 aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag cagatgccag
    16321 tagctggtca tttcgatcag ttcatcgtaa tagctggaga tcccttccgc acgtttctgg
    16381 atctgctcgt tggtaacgat atcgtcaaag acaactgacg gatcgttaac gatattacgg
    16441 atgatatggg tataggagcg ggaatgaatc gtttctgaga acgcccaggt ttctacccag
    16501 gtttccagtt ccgggatcga gatcagcggc agcagtgcca cgttcggact gcgaccctga
    16561 atggaatcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt ttcgtgttca
    16621 ggcagtgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg acgccagaag
    16681 aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg atcgtagcga
    16741 gccacgttga ccggctgacc aaagaacatc ggttctttga gctgatcatt tttcgtctgt
    16801 gaaaaggtgg tatatgccat gaatgtgtcc tgttaggagt tttatgccgg atgcgcttcg
    16861 cttatccggc ctacaaaccg catcgtaggc tgggtagggc gttcatgccg catctgggca
    16921 tccagtgccg gatgcgcttc gcttatccgg cctacaaatt ggacccgtag gccgggtagg
    16981 gcgctcacgc cgcatccggc atcacaatat cagatcttac atgcgccgct ttcgcagcca
    17041 tcgtcctgga ttgacggcac cagatcgtcc tgcgcgtctt ctgcgccgtc acgggtgttc
    17101 tgataataca gcgttttgac cccgaatttg taggcggtga gcaggtcttt cagcaactgt
    17161 tgcatcggca cttttcctga cgggaaacgt gacggatcgt agttggtgtt agcagagatc
    17221 gactgatcga taaatttctg catgataccc accagttgca ggtagccatc gttacccggc
    17281 atttcccaca gcagctcata ggcgtcgtgc agatgctcgt agtccggcac cacctggcgc
    17341 agaataccgt ctttcgacgc tttgatgctg acgtaaccgc gcggcggttc aataccgtta
    17401 gtggcattag agatctgcga agaagtctcg gacggcatca gggcagaaag cgtggagtta
    17461 cgcaggccgt gcgttttgat tgactcacgc agtgcttccc agtcgtaatg cagcggctca
    17521 ttagcgatgg catccagatc tttcttatag gtatcgatcg gcaggatccc tttcgcgtaa
    17581 gtggtttcgt taaaccacgg gcacgcgcct tgctctttcg ccagctcatt agacgctttc
    17641 agcaggtaat actgaatggc ttcgaaggtt ttatgcgtca ggttgttggc gctgccgtcg
    17701 gagtagcgtt taccgtgctt cgccaggtag taagcgaagt tgatcacacc aatacccagc
    17761 gtacgacgac ccatcgctcc acgtttggcg gccgggatcg ggtaatcctg ataatccagc
    17821 agcgcgtcga gtgcgcgtac tgccagaatt gccagctctt ccagttcatc caggctgtta
    17881 attgcgccca ggttgaaagc agacagcgta cacagcgcga tttcaccgtt ctcgtcgttg
    17941 acgtcgttca gcggtttggt cggcagggcg atttccaggc acaggttaga ctggcgcact
    18001 ggcgcgatgg ccggatcaaa cgggctatgg gtattgcagt ggtcaacgtt ctgaatatag
    18061 atacgaccgg tagacgcacg ttcctgcatc atcagcgaga acagctcaac ggctttcaca
    18121 cgctgcttgc ggatgctgtc gtctttctca tatttggtat acagacgttc aaactcttcc
    18181 tgatcggcga agaacgcgtc gtacagcccc ggtacgtcgg acgggctgaa cagagtgata
    18241 tcttcacctt tcagcagacg ggtatacatc agtttgttga tttgaacccc gtagtccata
    18301 tgacgcacgc ggttgccttc aaccccacgg ttgtttttca acaccagcag gctttccact
    18361 tccagatgcc acatcgggta gaacagcgtt gccgcaccgc cgcgcacacc gccctgagag
    18421 caggatttca ccgcggtctg gaaatgtttg tagaacggaa tacaaccggt atggaacgct
    18481 tcgccaccgc gaatcgggct acccagcgca cgaatacgcc cggcgttgat gccgatcccg
    18541 gcacgctggg aaacgtattt aacaatcgcg ctggaggtgg cgttaatgga atccaggctg
    18601 tcgccgcact cgatcagcac gcaggagctg aactgacgag ttggggtacg cacgccggac
    18661 atgattggcg tcggcagcga aattttaaat gtggaaaccg cgtcgtaaaa acgcttcacg
    18721 tattgcaggc gcgtttcacg cgggtagttc gagaacaggc acgcggcaac cagaatataa
    18781 aggaactggg cgctttcgta gatttcgccg gtcacgcggt tctgtaccag atatttgcct
    18841 tccagctgct taacggcagc ataagagaag gtcatatcac ggtcgtgatc gataaacgtg
    18901 tccatctgct tgaactcttc ttccgtgtag tcttccagca gatgattatc gtatttgccc
    18961 atctcgacca ttttcaccac atggtcgtac agcgcaggcg gctcaaactg accgtaggct
    19021 tttttacgca ggtggaagat cgccagacgt gcggcgagat actgataatc cggcgcatca
    19081 cgggagatca ggtctgcggc agccttgata atagtttcgt ggatgtcaga ggtcttgata
    19141 ccgtcataaa actgaatgtg ggagcgcagc tcgacctggg aaatcgaaac gttatgcagt
    19201 ccttctgccg cccaatccag aacgcgatgg attttgtcga gattgatgcg ctctgtgcta
    19261 ccgtcgcgct ttgtcaccag cagattctga ttcatgtatg tcgtacctgt ttttcaatag
    19321 cttcatgcag actacctgta gtgagggaag ctgctaagaa cactatatat agggggtatg
    19381 tttgggattc actgcaagat agtgtgaaac tggccctctt gcaagtgcat aactttgtgg
    19441 ataactcagg aaggaaaaag tggcttccgc gcaccttagg tcagacaagg tgtccgggaa
    19501 agtcaatggg aagagaaaaa tttgttaaaa ataaccgttc ggtgaattct tgaacggtaa
    19561 aataggatag gtaaattgat atagatggtt tattaatctt gttgctaatg attgccaaaa
    19621 ttgatctagg cacaagtcgt cacataatct gatgtatcag ttgtggtcaa tatataagcg
    19681 agtgtaagcg attgttgagt cggaaatggt tgttttatat tcggttattg ttaaatacta
    19741 agccaatttg t
//
LOCUS       DAZGJT010000088        19725 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZGJT010000088 DAZGJT010000000
VERSION     DAZGJT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19725
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.088"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(341..417)
                     /locus_tag="ACHKFK_003499"
     tRNA            complement(341..417)
                     /locus_tag="ACHKFK_003499"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(381..383),aa:Met,seq:cat)
     gene            626..1723
                     /gene="mltA"
                     /locus_tag="ACHKFK_003500"
     CDS             626..1723
                     /gene="mltA"
                     /locus_tag="ACHKFK_003500"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO9053763.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGVDNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1800..2606
                     /gene="tcdA"
                     /locus_tag="ACHKFK_003501"
     CDS             1800..2606
                     /gene="tcdA"
                     /locus_tag="ACHKFK_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO9053764.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVVQYMNAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2657..3100)
                     /gene="csdE"
                     /locus_tag="ACHKFK_003502"
     CDS             complement(2657..3100)
                     /gene="csdE"
                     /locus_tag="ACHKFK_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO9053765.1"
                     /translation="MTNLQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPEELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(3100..4305)
                     /gene="csdA"
                     /locus_tag="ACHKFK_003503"
     CDS             complement(3100..4305)
                     /gene="csdA"
                     /locus_tag="ACHKFK_003503"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO9053766.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNASDDKNIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKPELLEAMSPWLGGGKMVHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLTDYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4497..4724
                     /gene="ygdI"
                     /locus_tag="ACHKFK_003504"
     CDS             4497..4724
                     /gene="ygdI"
                     /locus_tag="ACHKFK_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO9053767.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            5075..5992
                     /gene="gcvA"
                     /locus_tag="ACHKFK_003505"
     CDS             5075..5992
                     /gene="gcvA"
                     /locus_tag="ACHKFK_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO9053768.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            6011..6406
                     /gene="ygdD"
                     /locus_tag="ACHKFK_003506"
     CDS             6011..6406
                     /gene="ygdD"
                     /locus_tag="ACHKFK_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO9053769.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6399..7499
                     /gene="rlmM"
                     /locus_tag="ACHKFK_003507"
     CDS             6399..7499
                     /gene="rlmM"
                     /locus_tag="ACHKFK_003507"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO9053770.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSSNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7543..8274)
                     /gene="fucR"
                     /locus_tag="ACHKFK_003508"
     CDS             complement(7543..8274)
                     /gene="fucR"
                     /locus_tag="ACHKFK_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO9053771.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKREHIKLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVTTSLVTA"
     gene            complement(8332..8754)
                     /gene="fucU"
                     /locus_tag="ACHKFK_003509"
     CDS             complement(8332..8754)
                     /gene="fucU"
                     /locus_tag="ACHKFK_003509"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO9053772.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8756..10174)
                     /gene="fucK"
                     /locus_tag="ACHKFK_003510"
     CDS             complement(8756..10174)
                     /gene="fucK"
                     /locus_tag="ACHKFK_003510"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO9053773.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTDCHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISEQQLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQILIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTTQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(10283..12058)
                     /gene="fucI"
                     /locus_tag="ACHKFK_003511"
     CDS             complement(10283..12058)
                     /gene="fucI"
                     /locus_tag="ACHKFK_003511"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO9053774.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVRKPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGKPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(12091..13407)
                     /gene="fucP"
                     /locus_tag="ACHKFK_003512"
     CDS             complement(12091..13407)
                     /gene="fucP"
                     /locus_tag="ACHKFK_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO9053775.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     FGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSTYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLAHIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13954..14601
                     /gene="fucA"
                     /locus_tag="ACHKFK_003513"
     CDS             13954..14601
                     /gene="fucA"
                     /locus_tag="ACHKFK_003513"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO9053776.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EANLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14629..15777
                     /gene="fucO"
                     /locus_tag="ACHKFK_003514"
     CDS             14629..15777
                     /gene="fucO"
                     /locus_tag="ACHKFK_003514"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO9053777.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVIDKMDAAGLAWAIYDGVVPNPTITVVKEGLDVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15882..16637)
                     /gene="xni"
                     /locus_tag="ACHKFK_003515"
     CDS             complement(15882..16637)
                     /gene="xni"
                     /locus_tag="ACHKFK_003515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO9053778.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHNEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16749..18116)
                     /gene="sdaB"
                     /locus_tag="ACHKFK_003516"
     CDS             complement(16749..18116)
                     /gene="sdaB"
                     /locus_tag="ACHKFK_003516"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO9053779.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLFLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(18174..19463)
                     /gene="sdaC"
                     /locus_tag="ACHKFK_003517"
     CDS             complement(18174..19463)
                     /gene="sdaC"
                     /locus_tag="ACHKFK_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO9053780.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPIIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4869 a         4827 c         5183 g         4846 t
ORIGIN      
        1 ttaagatatt cattaaactt attgtccggg ctttcacaaa aaattattaa tttttctact
       61 aattaaaaag ttgtgtgctt tttgtacaat gattatataa gcgtagcgcc ctgttgtgat
      121 attgaaatgt taacataacc aaaataaatt gatttataga atatataaaa ttcattaatg
      181 gggtatatgt ttaatatata aataagatgg atggctttga tggagggata ttaacggttt
      241 actcattgtc tcaggcttca ctgaaacggg taaggaagcg taaaaacgaa aaaaccgcaa
      301 gccaatagta gctttgcggt ttgtcagcat tctaataaat tggttgcggg ggccggattt
      361 gaaccgacga ccttcgggtt atgagcccga cgagctacca ggctgctcca ccccgcgtcc
      421 gtggatgcgc actatactct gctcgcgttt tcatgcaacc ttttttttac cccgatcacg
      481 gattcggcat gattttgaac aaaaacaaat cactaaagcc tattttttgt gcaaaatttg
      541 cgtcagggag aggctgctca tttcattaaa cgcggtggtt tgttatcttc gttgcgcctt
      601 attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc ttcttatggg
      661 cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg gacagcaata
      721 taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg ccgttggcgc
      781 gccgattaat gccggtgatt ttgccgagca aattaaccat atccgtaatt cgtcaccgcg
      841 tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc gcgcaggcgg
      901 tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag gtgtcgacaa
      961 ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc gccatacccg
     1021 tcagggcgag ttccagtatc cgatttaccg tatgccgcca aagcgtggtc gtctgccgtc
     1081 tcgtgcggag atctacgcag gagctctgag tgataaatat attctcgctt acagtaactc
     1141 cctgatggat aacttcatta tggatgtgca gggtagcggg tatatcgact ttggtgatgg
     1201 cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc gcagcattgg
     1261 taaggtgttg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc aggcgattcg
     1321 tcactggggc gaaacacata gtgaagctga ggttcgcgag ctgctggaac agaacccgtc
     1381 tttcgtcttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg cggtgccgct
     1441 ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta ctaccttgct
     1501 ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg aattgcgtct
     1561 gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata tctatcaagg
     1621 gatcgggccg gaagccggac accgcgcagg ttggtacaac cactatggac gtgtctgggt
     1681 gctgaaaacc gctccgggcg caggtaacgt ctttagcggc tgatatgata ttctgtatgc
     1741 cgtttttacg gatatgacag ggtgaggggt aacctcaccc tttttaattc tgaggtgcta
     1801 tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg cgtctgtatg
     1861 gtgaaaaagc attgcaactg tttgctgacg cgcatatttg tgtggttggt atcggtggtg
     1921 tcggttcctg ggcggcggaa gcgctagcgc gcacggggat tggcgcaatc acgcttatcg
     1981 atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg cgcgataacg
     2041 tcgggctggc aaaagcggaa gtaatggcgg agcgtattcg ccagattaac ccggagtgcc
     2101 gtgtaacggt ggtggatgac ttcgtgacgc cggataacgt agtgcaatat atgaatgccg
     2161 gttattcgta cgtgattgat gctattgata gtgtacggcc caaagcggcg ctgattgctt
     2221 attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgctggtggg cagattgacc
     2281 cgacgcagat tcaggttacc gatctggcga aaacgattca ggacccgttg gcggcgaagt
     2341 tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt aagctcggcg
     2401 tggattgcgt gttttctacc gaagcgctgg tgtacccgca gtcagacggc accgtctgtg
     2461 cgatgaaagc cacggcggaa gggccaaagc ggatggattg tgcctcagga tttggcgcgg
     2521 cgacgatggt gaccgccact tttggctttg ttgcggtttc tcatgcgctg aagaagatga
     2581 tggcgaaagc ggcgcgtcag gggtaaacaa tcaggtcgga tgcgacgcga acgtcttatc
     2641 cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aatcgcctcg cttaacgcat
     2701 tcaacccctg gctgcgtgag gcgctaagtt gcgcacgtaa tcccagctca tcaaacaatg
     2761 ccagcggcga ctgtgcctgc aattcggcgg cggttttccc ctcaacggca gtcaacaaca
     2821 ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaaatgc atcttgccgt
     2881 tttcagccac ggtatatccc agccagacgc ggttttcgca tccggcaatc tctttagcct
     2941 gcgcttttaa ctcctctggc aaagccggaa gctgtttccc cagcatgatc aactggcgat
     3001 atttatcttc ccattgcgtc aacggtgcga aggtattgcg taacgtttct gcggttacgg
     3061 ttgtgccgaa cggatgtccg gcgaattgca ggtttgtcat taatccacca ataattccag
     3121 cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat atggcgcaaa
     3181 tgaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag cgcaatgctg
     3241 cccggcgcgc agggcaatac cgtactccgc cagcagtgtc accatatcgc tgtgatgaac
     3301 tccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac gaaagcctgg
     3361 acgtttcgcc agcgcttctt ctgcaagcgt tgctaagcta cggctccagc tttcggcctg
     3421 gttgatatcg taatctgtca gccattccag cgccgcgctt aatcctatga caccagcgac
     3481 atttggcgtt cctgcttcca gtttccacgg cgcagattga gtcgtgaagc cgtcaaaact
     3541 cacttcgtga accattttgc cgccgcccag ccagggcgac atcgcttcca gcagttctgg
     3601 tttgccatac agcacgccga tgcctgtcgg accatacagt ttgtgacctg aaaaagcata
     3661 gaaatcaata tccagttgct gaacatccgc ggggaaatgc accgccccct gagcaccatc
     3721 aaccataacc accatcccgg ctgaatgagc aaaagtaatc gctctcgcca gatccgggca
     3781 accgccagtg acgttcgaca tctggcccaa cgccagaatc cgactacggg gagtaatcag
     3841 ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt tcaccacttt
     3901 ggcccccgtt tgttgggcga ccatcagcca ggggacgagg ttggcgtggt gttctgccac
     3961 actgacaata atttcatcgc ccggttgcag acgcggacgg gcatagcatt gcgccaccat
     4021 gttgatggat tcagtggtgc cgcgcgtcca gacgatattt ttgtcatccg atgcattcag
     4081 taattgtgcc actttttccc gcgcagcttc gtaacgcgcg gtcaggcgtt gggcttcggc
     4141 aaactggctg cgatggacgt ttccggcgct caggctataa aactgtcggg tagcttcaac
     4201 cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg catcctgtag
     4261 cgcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac tcctcggctt
     4321 gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa ccatcagaat
     4381 tatctggata tcctggcgaa gccgcgtcag taacatatgc tgaatagtgt taggcgttaa
     4441 ttttagcctg ttaatcaata ctattaagat ttattaatcg ctttaaggag aagatgatga
     4501 aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc gcctgttccg
     4561 gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac ggcaaaccac
     4621 agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac aaacagcaga
     4681 tcaaccgtac tgacgtgaaa gagatggtcg aactggatca gtaattcgcg atcgcaaggt
     4741 aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga cagggtaaat
     4801 gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga ccaattgcaa
     4861 acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg cattccgata
     4921 aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg tagaagctca
     4981 acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt gtgatctaat
     5041 tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac caccgctaaa
     5101 tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg cagcagaaga
     5161 gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg attttctggg
     5221 gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag ggcaaagcta
     5281 tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta aactccaggc
     5341 ccgtagcgct aagggggcgt tgacggtcag tttactcccc agtttcgcca ttcattggtt
     5401 ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc gaatccaggc
     5461 ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat tttatggtcg
     5521 gggcaactgg ccggggctac gggtggaaaa actgtacgct gaatatttat tgccggtgtg
     5581 ctcgccgcta ctgctgactg gcgaaaaacc cttgaagacc ccggaagatc tggctaaaca
     5641 tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac agttggggtt
     5701 aaatcatatc aacgttcagc aagggccaat ttttagccat agcgccatgg tgctgcaagc
     5761 ggctatccac gggcagggag tggcgctggc aaataacgtg atggcgcaat ctgaaatcga
     5821 ggccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg ctttttatct
     5881 ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc aatggatcct
     5941 ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat aatttacgta
     6001 gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg gcttcatttt
     6061 tgtagctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg ccgttgagat
     6121 gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga tcttaggtct
     6181 ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg ttttcctcgc
     6241 gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcgctgtccc atctgcgttt
     6301 gtgggcgttt gtcacaccgg ttggcggcgt gagcttcctt gcgggctggg cgttaatgtt
     6361 agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt gtattgctgt
     6421 gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc ggccagcggg
     6481 aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat gaatgttatc
     6541 aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta atttttgccc
     6601 gccagtggtt tgtagtgggg gaactcctgc agcatttgcc gccagaagat cgtattaccc
     6661 ccattgtcgg catgttacag ggcgtagtag agaagggcgg tgaactgcgt gttgaagttg
     6721 ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaattcacc gtgccgctac
     6781 gcgctgcctt gcgtgatgcg ggggtgctgg cgaactatga aacgccgaag cgtccggttg
     6841 tgcatgtatt ctttattgca ccaggctgtt gctatactgg ttactcatac agcagcaata
     6901 attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagatgcg ccgagtcgtt
     6961 ccacgctcaa actggaagag gcatttcatg tgtttattcc cgcggatgag tgggatgaac
     7021 gcctggcgaa cgggatgtgg gcggtggatt taggtgcttg cccaggcggc tggacctacc
     7081 aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg gcgcaaagtc
     7141 tgatggatac cgggcaggta acgtggctgc gggaagacgg tttcaaattc cgtccgacac
     7201 gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggcgaaa gttgcggcat
     7261 taatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaac ctcaaactgc
     7321 cgatgaaaaa acgctacgaa gaagtgtcac acaatctggc gtatattcag gcacagcttg
     7381 atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat cgcgaagaag
     7441 tgacggtgca cgtccgccgt atctgggctg cggtgggtgg tcgtcgcgac gagcgataac
     7501 aggtaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg ttaccaaaga
     7561 agttgtaacc tggcgctcat cagaaataat gtgcgtgacc tcatccagat gcccgatgcg
     7621 ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt gcgcggcacg
     7681 ttttaataac attgatttgt aatcagcgtt gatggcattg gagtcccaca gtgcgccgct
     7741 gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt taagttggga
     7801 aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc cggaactgat
     7861 cagtttaatg tgttcgcgtt taccgagttc atggcaaatc ggatggctat tggtgaagac
     7921 ctggatgttg atgtcaggca actggcgtgc cagataccag caggttgaac tggcgtctaa
     7981 ggctatcacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat ctgctttatg
     8041 cgcataatgg cttttcagcc ggatgtgaaa gggatcgcca ctgtcttgat tttgacggtg
     8101 gatatattta gcgcgcccat gattgcgcag aattttaccc tgcgtctgta attcattgag
     8161 atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt ccgtggtcag
     8221 gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt tcatattttt
     8281 caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag attacggtgt
     8341 tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga taacgatcgc
     8401 aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga tgtcaggaca
     8461 gggggcttgt agtgaaagcg cattacgata acgtcgttct acttcaggat cgagagtgtc
     8521 accttctacc gccgccatca tcaccagcgg tggtgcataa ctgtccagct caaataacgg
     8581 gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca cctgcggtcc
     8641 catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat gccccatctc
     8701 tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca gcatttcaca
     8761 cctcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga taatgaatct
     8821 gtgcacgggc ttcttccggg ctgttaaatt cccctacgcc ataccaacca aacagcgcag
     8881 ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca agcatattgg
     8941 ctttaatctg attccacaat gtgttgcgac ttcctccgcc gactaacaat aattcagagg
     9001 ctttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc gtggttaacc
     9061 cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgttacg ccttgccagc
     9121 cagcgttctg gcacgacaat aaatcacact gcattttcac tccatctgcg ccaggcgcga
     9181 tcaggcgagc ttcttcaatc aatatttgcc agggagtttc agccgtccag aacagttttc
     9241 tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac aatcctgcct
     9301 gactatccag ttcgcaggtc gaaccggcgt actgacttaa cagcgaagta tcaacctggg
     9361 cgctgcggac cattaaaatt tcccatgtac cggaagagag cacgggttca ttttgttcgg
     9421 caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc ggtatgccaa
     9481 cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt tcacccgctt
     9541 ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt tgcggactga
     9601 aatcgcgttg ctggatatcc agcatctggc tggttccggc catcgtgata tcagtagtga
     9661 attcgccggt taaacggtgg ttaatcagcg acgaaataaa gagccaggcg tgcgcgcgtt
     9721 ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta ttgaaactaa
     9781 aggctccgac tccagaaata gcctgcaact gctgttcgga gatcaaccgt tcaatattgt
     9841 ccattaccgc ggctgttcgc ggacatttcc agctaatgat cggatagagc agattgcctt
     9901 gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg cggatgtggc
     9961 aatcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc aaaatggcgt
    10021 ctaaagacca ctggtgccag gtgttgtttt ccatcgcgat atcgctggca ttaggcgttg
    10081 aggcgcgggc aacaattttt ccctgccgat tgaccgcgat ggccctgaca ttggtcgcgc
    10141 cacagtcgag taccaggata acttcttgtt tcataatcgc tcctgcaata tagccggata
    10201 acattgctta tccggctaac cactcttgct tgttcaggcc cggtagccgg agcgaccggg
    10261 catcacatca gggagtaatg tattaacgct tgtacaacgg gccgtagttc tggcaagcgc
    10321 ggtaatcctg gccttcaata tccatgccgt gcgcggccca ggcagaagga cgatacactt
    10381 tggtctcttc aacgttgtgc atacataccg ggatacgcag catggaggca agagtgataa
    10441 agtctgcgcc aacgtggccg atggtcagaa ccccgtggtt agcgccccag ttcgccatta
    10501 ccgagtacac atccgtaaac ggccctttac cggtgaggcg cggtgcaaac caggtggttg
    10561 gccaggttga gttggtgcgt ttgttgagga tgtcatgcac atccttcggc aattccacgc
    10621 tccagccttc cgcgatttgc agtaccggtc ccaagccttt gatgatgttg actcgagtca
    10681 tagtgaacgg gacgccgcct tcggtaagga agcgagaaga gtaaccgccg ccacggaagt
    10741 attcgtggat cgccgggcac cattcggtag cggcgaggca agcgtcagcc tcttgctgag
    10801 agatttccca gtgcggcttc atcgtcggtt taccttcgct gtcgcgttgt ttacaggagc
    10861 cgtccagcgc agcagaaccg gagttgatca aatggatgat gccgtgttct gccagcccgt
    10921 ccagtttatg ccccgttaca cgctcaattg cttctggtga ccagtaggta cggacatcgg
    10981 caaatacctg agcggtgccg gtgagctggt ggcccattaa cattgccacg ccgttaagac
    11041 tgtcgttttc ggtcgccacg acaaacggtt tgcgcacgcc attccagtca aatgaactgt
    11101 tgaggatcgc ttcggcggtg tcgccattgg gatattgatc ggtccagtga cgttgcccct
    11161 ggaagcccgc agcgatggcg ttgtagccaa gtgattcttc cacgcggcca atatcggcca
    11221 gtttgctgtt gccttgcatc atgtcgcgga tacacatcgc catcagtaaa ctttcgcgca
    11281 gaactgcgcg gctttgctcg gcatttcgtt gatactgttt gttattttca tcttcgccat
    11341 agcggaagtt tttatcagcc caggccagcg ccatttccag ttcggcttcg tcgtaaatct
    11401 tctgatcgat acggcgacgc agttcggtca tatccaccgc ctggactttc attcccagcc
    11461 aggattcaaa gaagttgtga tcaacaatag aaccggcgat acccatcgaa acgccgccta
    11521 gcgacagata gcttttacct ttcatgctgg cgacggccaa accggcgcgg gcaaaacgca
    11581 gcagtttttc ttcaacatcg gcaggaatcg atgtgtcatc ggcatcctga acgtcatgac
    11641 cgtaaatgga gaatgccggg atgcctttct ggctgtgagc tgccagagcc gctgccaggt
    11701 aaacagcgcc gggacgttca gtgccgttaa agccccaaat ggctttcggg cgggttggat
    11761 ccatgtcgat ggtttcactg ccatagcacc agcaaggggt taccgtaatg gtgaggccta
    11821 cattctgact gctgaatttt tcttcgcaag cagcggcttc agccataccc gcgatacagg
    11881 tatcggaaat gacacactcg acggcagctc cacaggcatg gcgcagtttc tcggtcagca
    11941 gtgcggccgt ggctttcgcc atattcattg tttgttcttc aagcgactca cgaacaccca
    12001 tgcgacgacc gtcaataacc gggcggatac caattttcgg taagctgatt tttttcatta
    12061 cagattcctc actttattcg gaaaatatgt tcagttagtt gccgtttgag aacggaaacg
    12121 ggcaaagata aagatgactg cgaagcagag tgccgggatc agttcggctg tggggatgtt
    12181 gcccgcagcg tcactgacaa accccatgac cggagtgaca ataccgccgc caataatggt
    12241 cataacgatg aaggacgaac cgtatttggt gtcctggccg agattcttaa tgcccagcga
    12301 gaagattgtt gggtactgaa tcgacataaa ggcactgcat aaagtcaggg ctattaaacc
    12361 cacatgaccg ccagcgaagg ctgagatcag gcacagtgcc atagcaatta atgcgtaggc
    12421 tgccaggact ttgtgtggtg cgaagcgact gatgagccag gtaccggtga aacgaccaat
    12481 aaagaagcac accatggttc cggttaaata gttagcggca aagcctgctg tcatacctgg
    12541 aatttcttct acagcgtagc gaatcaaata gctccagcag gccgtttgtg cgccgacgta
    12601 gcagaattgc gctaatactg cccagcgcca gtggcgaata tgcgccaggc gagaaagcga
    12661 tgcggagaac gatccttgtt tggcgtcact gtgattatca ctctgcaatg ccgggaattt
    12721 cgtcagcatg atcagcaggg cgaccagtaa cacgatagcc acgatgatca tataaggtgt
    12781 ctgtaccgat aatactaggc tgtgtttata cgtactcaat tgctctggag acattttatc
    12841 gagaacgtct tgcgattgat gtggcacgtt agacaaaata agactttgcc caaagacaac
    12901 cgcgataatt gcgccaaatg agttaaatgt ttgcgcaaga tttaagcgga agtgaccact
    12961 gctttccggc cctaataccg taacaaaagg gtttgcggca gtttccagac aacctaatcc
    13021 ggctgcaata ataaataggc caactaaaaa caaggtgtag ttcattattt ctgcggcggg
    13081 ccagaataat gcagcgccga aagcatataa aaacaatccg gtaataatcc ctgctttata
    13141 actgagtttt ttcatcaata tcccggcagg gattgggata atgaaataac caaagtaaaa
    13201 ggccgattgg atcaggccag cctggaaatt tgtcagcgta aaagcctgct ggaattgagg
    13261 caataaaatg tcgttaaggt tattggctac cgcccaaaga aaaaacagtg agcacagcag
    13321 cgcgaatgga ataatgtaac ttctgctttg ccctgcatct ttatctaccg cacggtaact
    13381 ctgcgtttgt attgatgtgt ttcccatagc atcctcttag gttcagaagc ttaagcacgt
    13441 aatcggttat gttttcaaaa gtgcaaaacc agcgagttcg ggcttcatat cagtgaatat
    13501 ttgcctgtct gaaatgaact catggattca ttttcaaata aaaaacgctc tgtaatatga
    13561 cggcggtcac acttattagc cggaaggtga ctttatgtga ctaccatcac tttaataatc
    13621 gaaatttcat gcccgtttca gaaaataaat cataaaaaac acaaaccaga tgaccgtatt
    13681 aattgtttgt gccgaaaacg ggcattcctg gcagggatga gtagaaaaat gaccgttttg
    13741 ataaatattt ttaagagcgg tcatttttaa tggggttata aaaagagaac ttgcagttat
    13801 ttactgtgat tagttgaacc aggtcacaaa atataagctt gtgaatttct ttctggttaa
    13861 tcaggaagaa gatggtgggt aattaaacgg ctaattcaat agtgtgaaag gaacaacatt
    13921 attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact tgctcgtcag
    13981 attattgaca cttgcctgga aatgactcgc ctggggctga accaggggac agcgggtaac
    14041 gttagtgtac gttatcagga tgggatgctg attacgccca caggtattcc atatgaaaaa
    14101 ctgacggagt cgcatattgt ctttattgat ggcaacggca aacatgagga aggaaagctc
    14161 ccctcaagcg agtggcgttt ccatatggcg gcctatcaaa gcagaccgga tgccaacgcg
    14221 gttgttcaca atcatgccgt tcattgtacg gcagtttcca ttcttaaccg accgatcccc
    14281 gctattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc gccttatgcg
    14341 acctttggaa cacgcgaact ttctgaacat gttgcgctgg ctctcaaaaa tcgtaaggca
    14401 actttgttac aacatcatgg gcttatcgct tgtgaggcga atctggagaa agcgttatgg
    14461 ctggcgcatg aagttgaagt gctggcacaa ctttacctga cgaccctggc gattacggac
    14521 ccggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt caaaacctac
    14581 gggttacgga ttgaagagta atttcgtaaa gcaacaagga gaaggatgat ggctaacaga
    14641 atgattctga acgaaacggc atggtttggt cgaggtgctg ttggagcttt aaccgatgaa
    14701 gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct ggtgcaatgc
    14761 ggcgtggtgg cgaaagtgat cgataagatg gatgccgcag gactggcatg ggcgatttac
    14821 gacggcgtag tacctaaccc aacaattact gtcgtcaaag aagggctcga tgtattccag
    14881 aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga tacgtgtaaa
    14941 gcgattggca ttatcagcaa caacccggag tttgccgatg tgcgtagcct ggaagggctt
    15001 tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc aggcactgcg
    15061 gcagaagtga ccattaacta cgtgatcact gacgaagaga aacggcgcaa gtttgtttgc
    15121 gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat ggatggtatg
    15181 cctccagcgc tgaaagccgc gacgggtgtc gatgcgctca ctcatgctat tgaggggtat
    15241 attacccgtg gcgcgtgggc gctaaccgat gcgctgcaca ttaaagcgat tgaaatcatt
    15301 gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga aatggcgctc
    15361 gggcagtatg ttgcgggtat gggattctcg aatgttgggt tagggttggt gcatggtatg
    15421 gcgcatccac tgggcgcgtt ttacaacacg ccacacggtg ttgcaaatgc catcctgtta
    15481 ccgcatgtca tgcgctataa cgctgacttt accggtgaga agtaccgcga tatcgcgcgc
    15541 gttatgggcg tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc cgctgttgaa
    15601 gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttacgtga tgttggtgta
    15661 cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg taccggtggc
    15721 aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc ctggtaaata
    15781 cgctgatgtg ataatgccgg atgcgacgct agccgcgtct tatccggcat ataagtgcat
    15841 gaaattcaat gtgttgataa tcgtttgcat ccggcatgcc gttaccttac caaccgcaat
    15901 tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc gggcaatatc gcggcacaga
    15961 aacgccatct ctttatgggt ttctaatttt ttgcgccact tttcggcaac cgcatccaga
    16021 ttctcatata tcccttccag actctgaaac tcgaccagta gctgtgtggc gctttttggt
    16081 ccgatccctg caacacccgg tacctttgaa ctgctgatcc ccgcaagccc ccagtaatcg
    16141 ggcaactgct gcggttgaac gccaaattct ttatcgataa atggcgcatc cagccaacgt
    16201 ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta actgacagta gcctttatct
    16261 gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt tgaccgccag cgtggcggct
    16321 aagtcatcgg cttcgttgcc gctggcggac cagcacggga cgccgcgttg ctcaaaggcg
    16381 gcgcgtaatg caggcatctc gttgtgcaac tcttccggca ttggcggtcg acccgctttg
    16441 taatctggta aacgctgatg acgccagccg ctactgcggt tttcatcatc aaaaacggcg
    16501 accgcgtggg ttggctggct gtgcataatg agctgatcga gcgcgtgctg gcaggtttcg
    16561 acacagggcg aaccctgaac ggcatgaatg cgacgaataa gattcagtgc atcgacaata
    16621 agcaaatgaa cagccacgat aactccctta aagatcaggc gataagggta acattcgatg
    16681 ccagaacagg ctatggctgg atgagaaatc gggaagaggc ctcgcaaaaa gaggcctctg
    16741 gagagcgatt aatcgcaggc aacgattttc attgccaggc cgccgcgaga cgtttcacgg
    16801 tacttggcgt tcatatcttt acctgtttcg tacatggttt cgataacttt gtcgaggcag
    16861 acgcgtggct cgctggtacg gcgcagcgcc atacgtgcag cgtttactgc tttcactgcc
    16921 gcaatggcgt tacgctcgat gcacggcacc tgtacctgcc cggcgaccgg gtcacacgtc
    16981 agaccgaggt tgtgctccat ggcgatttcc gccgcgatgc atacctgcgc cgggcttgcg
    17041 cctaacagtt ctgccagacc agctgccgcc attgagcacg ccacgccgac ttcaccctgg
    17101 cagccgactt cggcaccaga aatcgaggcg ttcatcttat aaagagaacc aatggcgctg
    17161 gctaccagca ggtaacgagc cagtgagtta gcgttcactt cgcggataaa cttgtcgtag
    17221 tacgccagca cggccggaat aatcccgcac gcaccgttag tcggcgcagt caccacgcga
    17281 ccgccagcag cattctcttc gttcactgcc agtgcaaaca tgttgatcca gtcaacaacc
    17341 gccatcgggt cagtggtggt tttatcctgg ctgaccagca tccggcgtag tgccgcagca
    17401 cggcgtggaa cgcgcagttt gccaggtaac acgccttcgg tagaaatacc gcgctcaata
    17461 ccgccacgca tgacttccca gacgttcgcc aggtgctgtt ccagctcttc tttgctgtgc
    17521 agcgccagct cgtttttcat catcaggcca gagagtgaca gcccggtttc ctgacaatgt
    17581 ttttgcagat cggctgctga actgtacgga taaggaactt caaccggtgc gctatcctgc
    17641 tgaccaaaat gttcttcatc aacaataaag ccaccgccga tagagtaata agtctggctg
    17701 taaacgactt tatcgcccgc cagcgcggta atgcgcatac cgttttcatg cagaaaaagg
    17761 ttgtcggcat ggaagttcat gcactgatca accgggaact ccacttcatg ctgaccgttt
    17821 gccagcatca ggcgaccatg agtattcaca tcctgaataa aaccagggat ggaatcgata
    17881 tccacggtat ccggcaggtt acccgccaga cccataatta tggcgatatc tgtgtggtgg
    17941 cctttaccgg tcagagagag cgagccgtac acgtcaacca ccacgcgggt cacgtcttta
    18001 agcaggttac gggcaatcag atcgtcggta aattgtttac ccgctttcat tggtccaacg
    18061 gtatgagaac tggaagggcc aatgccgatt ttgaaaatat cgaatacgct aatcatagga
    18121 aatacatcgc gttaaaacgg aggaagcgcc gcccgaaagc ggcgcgaaag gacttagctg
    18181 aacagagagt agaagattgc ggagattgca atcagaccca tcacgacaac gaatacgttg
    18241 ctgatgtgac cgctgtattt acgcattgcc ggtactttct gaattgcgta catcggcatc
    18301 aggaacagga tcatcgcgat gattgggccg cccagggttt caatcatacc caggatgctc
    18361 gggttcaggg tggcaacaat ccaggtcgtt accagcatga acagcgcagt gatacggttc
    18421 agcttgttga tttcgataga cttaccttta ccacgcagag atttaatcac cataccgttg
    18481 aagccttcac gtgcgcccag gtagtggccg aggaaggatt tggtgatagc gataatcgcg
    18541 ataatcggag ccatccacgc gatgatcggc gcgttaaagt ggttagccag gtaagacaga
    18601 atcgagatgt tctgctcttt agccgcagcc aggtctgccg gagtcaggct cagtacgcag
    18661 ctgaagacga agaacattac ggtcagcacc atcatgatgt gtgcgaatgc caggatcttc
    18721 gagcatttct gttctgccat atcgccgtac tcttcacgct tcgcaacggc gaaagaagag
    18781 atgatcggag agtggttgaa cgagaacacc attaccggaa ttgccagcca cagtgtcatc
    18841 cacagaccgt ttccggttgc agatgcagtg tccagagaca gcgtttccag tgctgcgccg
    18901 ttccactgcg ggatcaggta cagagccagc agcatcagta cgccaacaaa cgggaatacc
    18961 agaatactca tcgctttaac gatcatctgc tcaccgaagc gaacgatggt catcataccc
    19021 acgatcagga tcagcgacag aatcgcacgc ggcggtggcg tcatacccag ctggtgagac
    19081 atgaagcttt caactgtatt ggtgattgcc acgctataaa ccagcaggat cgggtagata
    19141 gcgaagaagt agagcagagt aatcagttta cctgcgccaa taccaaagtg ttcttctaca
    19201 acctcggtga tgtcttcgcc cgggttttta ccagacagta cgaagcgagt caggccgcgg
    19261 tgagcaaaaa acgtcatcgg gaacgcaagg atagccatga tgatcagcgg gatcataccg
    19321 ccaacaccag cgttgattgg caggaacagc acgcccgcgc cgattgccgt gccgtaaagg
    19381 cccagcatcc acatggtgtc tgtcttgcgc caggcactac gagagtcttt cgacgcaatc
    19441 gtgctggttt gagtcgtttc catctatttc tcctggagga atgcaaataa ttcagctttt
    19501 agccagttca gcccgggaag gtgcctggcc gtaaaaatga aatctgtcta tcggcgatgg
    19561 cttaatcatt ttccaccgta ataatttgag ggcggaaaga tacacacaag ttatgtatct
    19621 atcagtgatc ttgatcccaa atacaataat ctacttggaa gatagataat cacaacgctt
    19681 tactctgtaa atcaaaagta aaagagaatt taagggcaat attta
//
LOCUS       DAZGJT010000089        19298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZGJT010000089 DAZGJT010000000
VERSION     DAZGJT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.089"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(390..521)
                     /locus_tag="ACHKFK_003518"
                     /pseudo
     CDS             complement(390..521)
                     /locus_tag="ACHKFK_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            803..2161
                     /locus_tag="ACHKFK_003519"
     CDS             803..2161
                     /locus_tag="ACHKFK_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HFO9053781.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNHG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYQVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2400..3785)
                     /locus_tag="ACHKFK_003520"
                     /pseudo
     CDS             complement(2400..3785)
                     /locus_tag="ACHKFK_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3835..4182)
                     /gene="tnpB"
                     /locus_tag="ACHKFK_003521"
     CDS             complement(3835..4182)
                     /gene="tnpB"
                     /locus_tag="ACHKFK_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9053782.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(4179..4559)
                     /locus_tag="ACHKFK_003522"
     CDS             complement(4179..4559)
                     /locus_tag="ACHKFK_003522"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9053783.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4914..5348)
                     /locus_tag="ACHKFK_003523"
     CDS             complement(4914..5348)
                     /locus_tag="ACHKFK_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO9053784.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDENYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(5336..5737)
                     /locus_tag="ACHKFK_003524"
     CDS             complement(5336..5737)
                     /locus_tag="ACHKFK_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO9053785.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5997..6566)
                     /locus_tag="ACHKFK_003525"
     CDS             complement(5997..6566)
                     /locus_tag="ACHKFK_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO9053786.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            6765..6965
                     /locus_tag="ACHKFK_003526"
     CDS             6765..6965
                     /locus_tag="ACHKFK_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053787.1"
                     /translation="MVIRKKKCRDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVCILCSW"
     gene            complement(7313..7522)
                     /gene="hha"
                     /locus_tag="ACHKFK_003527"
     CDS             complement(7313..7522)
                     /gene="hha"
                     /locus_tag="ACHKFK_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO9053788.1"
                     /translation="MEVKTKEDWLYQFRRCSSRETLEKVISHTRYKLTPAELAFNSAV
                     DHRLAELTMNKLYDRVPASVWKYVT"
     gene            8221..8427
                     /locus_tag="ACHKFK_003528"
     CDS             8221..8427
                     /locus_tag="ACHKFK_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9053789.1"
                     /translation="MNTPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            8513..9085
                     /locus_tag="ACHKFK_003529"
     CDS             8513..9085
                     /locus_tag="ACHKFK_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053790.1"
                     /translation="MQKLENRCDLLLTQHQKWMASVTRFIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGTIISVAPQGLYQVVNPATPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGESRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELESWAVARKREKRELELMIDEYLLYAC"
     gene            9294..9401
                     /locus_tag="ACHKFK_003530"
                     /pseudo
     CDS             9294..9401
                     /locus_tag="ACHKFK_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612303.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            10578..11597
                     /locus_tag="ACHKFK_003531"
     CDS             10578..11597
                     /locus_tag="ACHKFK_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9053791.1"
                     /translation="MNNEQMLIKCKDPRKCRFYAEYLNHKYPNEFPQFAPDNPQNCIR
                     NVNSFLKEKYYKASNNSFGVFGSTAEEALSKEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            11707..12579
                     /locus_tag="ACHKFK_003532"
     CDS             11707..12579
                     /locus_tag="ACHKFK_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO9053792.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRAQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
     gene            12912..16034
                     /gene="agn43"
                     /locus_tag="ACHKFK_003533"
                     /gene_synonym="flu"
     CDS             12912..16034
                     /gene="agn43"
                     /locus_tag="ACHKFK_003533"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HFO9053793.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARTRGKRGGVAVALSLAA
                     VTSLPVLSADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSTGQMVGGTAESTTIN
                     NNGRQVIWSSGVSRDTLIYTGGDQTVHGEAHNTRLEGGNQYVHKYGLALNTVINEGGW
                     QVVKAGGTAGNTTINQNGELKVHAGGEASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VAGKADNVVLENGGRLDVLSGHTATNTRVDDGGTLDVRNGGAATTVSMGNGGVLLADS
                     GAAVSGTRSDGTAFRIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGSLA
                     GTTTLNNGATLTLSGKTVNNDTLTIREGDALLQGGALTGNGRVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNNSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLTSGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSARTEKFVPTTLQVKNLNGQNGTISLRVRPEMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSGTGLATTGKGIQVVEAINGATTEEGAFVQGNML
                     QAGAFNYTLNRDSDESWYLRSEERYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVNG
                     ENNSVRLSIQGGHLGHDNNGDIARGATPESSGSYGFVRLEGDLLRTEVAGMSLTTGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYMNLTHTSSGLWADIVAQGTRHSMKASS
                     GNNDFRARGWGWLGSLETGLPFSITDNLMLEPRLQYTWQGLSLDDGKDNAGYVKFGHG
                     SAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITLGVQAGYAHSINGSSAEGY
                     SSQATLNVTF"
     gene            16155..18671
                     /locus_tag="ACHKFK_003534"
     CDS             16155..18671
                     /locus_tag="ACHKFK_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053794.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFVLLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVALLWAASQIQALKRLTSAIRQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTECIWLTLISQRISRGELREFELADGNWLLDNAWYERNMAGFNEKLRES
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEAFPPELPA"
     gene            18747..19202
                     /locus_tag="ACHKFK_003535"
     CDS             18747..19202
                     /locus_tag="ACHKFK_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO9053795.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHHVSLPLSVFDPQDYHPLLITVSGKNVN"
BASE COUNT         4860 a         4976 c         4892 g         4570 t
ORIGIN      
        1 tgttcagagc gcacttaata ttatcattga cattacatca ggtgatattt gagattttac
       61 ataaaagata atctctcaag gagtcttatc tgaaaacgaa atatatagtc cctctcgttt
      121 tgtaatatcg attggctttg ttgaatcggt tacgtacccc ggagccggtc atattttcag
      181 gtaatacaca cgctttcaga catttcggct ttcgtcattt tggtatcatg ggcatagctt
      241 ctgctacctg agcatcataa tcacgcagct tcagtgaagc agtgaccacc tgtttaaccc
      301 ggtgtatcgt tatgatctta tccagcaata atggacacgc ggctaagtga accgccccgg
      361 gtttcctgga gagtgtttta tctgtgaact caggctgcca gatcatcgtt tccgatggaa
      421 gcataataag ctttttctgc ttctgccggt gggatatggc cctgcctttc cagcaatcgt
      481 cgattgttat accagtccac ccacgtgagt gtggtcagct catcatactg acacgtgcat
      541 tacccctacc gcgataatgc aaccgtacaa tatagggcgt ttcctaaacg cagccctcat
      601 cactccaatc caacaagaca cagcattttt gatcacgaag aaagacaaat aatttccgta
      661 aatgtttccg ttgttacaaa taacaacagt gttttacatt tctttggcct caggcccgcg
      721 catgttgcca ttcgatgtta ttttcctgac tcgtttcttc tttatggtgt agtgtgatct
      781 tcactttatg gatgctaaca atatgaaaag aaaaattatt cccgtgctaa tcggttgtac
      841 gctctctttc tctgccctgg cggcacaacc tacggctgag cgctatgtcg tcagttttcc
      901 agaaggcacg cacgtgaact acgccggcgc atttgccagt gcattcccga acggccttcc
      961 ggttgggata ggttctggcc tgctgttcac cggtaaacag ggcgatgccc tgacattcgc
     1021 aaccattacc gatcgcggtc cgaacgcgga ttcaccgaaa gaggggaaaa acgaaactaa
     1081 aattttcgtt acgccagatt tcgcgccgct gctgatgacg attcgtgtac aaaacggcaa
     1141 agcggaggcc atcgatcccc gtcctctgca tgacgataaa ggtgcgatca acggtctgcc
     1201 gctggcaagc gacgtgattg gctccaccaa tgaagtggcc ttcagcgaca cgttacatag
     1261 actgaagggg gataatcacg ggctggatac ggaagggatc actccggatg gtaaaggagg
     1321 ttactggctg tgcgatgagt atggcccgtt cctgattaac atcgacagta aagggaaaat
     1381 cctggcaatc cacggtccgc aggcggcgga aggggaaaaa gcgattgcgg gcggtttgcc
     1441 aaatatcctc aaatggcgtc aggcgaaccg cggctttgaa ggcctcaccc gtatgccgga
     1501 cgggcgtatt attgtcgccg tgcaaagtac gctggatatc gacgctaaga gcaagaaaaa
     1561 ggcgctgttt acgcgtctgg tgagcttcga tccggcaagc ggaaaaaccg cgatgtacgg
     1621 ttatcctatc gacagcgcgg cctacagcaa aaacagcgac gctaaaatcg gagacatcgt
     1681 ggcgctcgat aaccagcaca tcctgctgat cgaacaggga agagataaaa acaacagaat
     1741 gcgtaacctg atttacgagg tcgatctcaa taaggcgagc gacctgtcag gcttcgacaa
     1801 gccgggtgag tatccggagt ttgatgatga gaaaaccctg tcacagcgcg gcattaccct
     1861 tgcgcagaaa acgcaggttg ttgatctgcg ctccttgggc tggcagcagg agaaggctga
     1921 agggttggcg ctgatcgaca gcaaaacgct ggcggtggcg aacgataacg actttggcgt
     1981 gaaggtggcg atgcaacatc cggtcgaggg caagacattc aaggattatc aggtgaacgc
     2041 ggaaggaaag ctgacgcttg acgacaagca ggttgaaacg accctcagag tgaagccact
     2101 ggagaaaccg gagtctgaca gtgaactgtg gattgtgacg ctgccggaag cgttgaaata
     2161 agtttataag gtacggacag cagtatcgct tagtttttca aattcatgta tgtgaacaat
     2221 attggggttc cggcgtagac cctcaatgaa agcgtcacca cgtgaaaaca tgcctgcacg
     2281 gtttacacgt catcccatgg tggcagccgg ttaactttcc tgctacacca gtggaaaaag
     2341 gccataagca gtccgaacaa aagtaagcgt acagcgaggg ccgtattgac ggggatgtgt
     2401 tattcagctg gcagtgctat gcgccacgga agcagttcgc tgacccggtt gaccggccag
     2461 tctgctatga cgccaagcac atggcgaagg tagctttctg gatccacgtc attcagtttg
     2521 cacgtcccga tcaggctgta cagtagcgct ccccgctcac caccatggcc agagccgaag
     2581 aacagggtct tcatcttttc acgcaaccag ctttccaggg atttcaacag cggtttcgtt
     2641 tttcgctgac gttcagcaag ccgctgctct gccggcattc cccttatatc cgcctctatg
     2701 gcgtacaact gaccgatctg ctccagggct tcttccgtca gtgctgacgg gatgcggacg
     2761 tgcacatcgt ggatctttca gcgggcatga gcccagcagg cagcttccgt tatcccacca
     2821 ttgcgataca gctcgttgaa cccggcgtac gcatccgctt gcagcacacc gctgaagcaa
     2881 gcaagatgag tctgcggatg gatgcctttt ctgtccgggc tgtaagcgaa cctcactgca
     2941 ggtgccaacg ctgacccggc attgcggtca tcacgaacat acgcccacaa ccgcccggtc
     3001 ttcgtcttct tattacccgg cagcagtacc tggaccgggg tatcatcggc atggagtttg
     3061 ccgtcagtca tgacatagcc atgaagcgcc tcttccagcg gagacagcag ccggcagcat
     3121 gcatccaccc agcccgacag cagtgaacgg ctcagctcca caccttgccg gccgtatatt
     3181 tctgactggc gatacagcgg ggtgtgctct gcatacttcg aggtcagcac gcgggccagc
     3241 agccccggtc cggcgatacc ccgctcgatg ggccgcgaag gtgcaggtgc ctgcacgatg
     3301 gtatcgcact gagtacaggc atgtttttcc cgtaccgtcc ggataacccg gaaggcgcta
     3361 cgcatcaact ccagctgttc ggcggtatcc tcgcccagat agctcagtga accgccgcag
     3421 ttcgggcagc acggcgccgc aggcaacagt cgcttttcgt cacggggtag tgattcaggg
     3481 aacggcttac gggtgcgggt ctgacgcaac ggacgctgta ctgccgggtc atacacccta
     3541 ccagtcagcg tatcgctctc tttctgaagc cggttcagat cggcttccat ttgtgcgata
     3601 cggcgggaga ctttttcgga acgactgccg aagttcatcc ggcggagttt atccagctgc
     3661 gcctgcagat ggtctatttc gcgctcccgg ttgctcagct tttccagcag ggcgtggatc
     3721 agcgcttcct gttcggccag gcgctgtttc agcaggaaga tgtcgtcaga agagatgtcg
     3781 ttcataagcc cgtattttac cgggcttatt ctgtggcaac caggataaag agatttacag
     3841 catggtcagg gaggtcagca gccgcttggg ctgtcgccag tcgatacctt ccagcagcat
     3901 cgccagctgc gcctgcgtaa ggaacacttt gccatcacgg gctgacggcc aggcgaagcg
     3961 cccacgctcc agccgtttgg tcaggaggca cagtccgtca ccggtggacc acagcagttt
     4021 aacctgactg ccgctgcggc cccggaaaat gaaaatatgg ccggacatgg gatcgtcttt
     4081 cagcgccgtt tgtactttcg cagccaggcc gttgaagcta tttctcatat cggtgatacc
     4141 ggcaaccagc caaattttgg tcccggaagg taacgggatc atcgcttcag ttcctgtatc
     4201 agcagagtca ggagcttttc gctgacattg ccattgaagc ggagcgtccc gtgccggaac
     4261 gttacctcac agctgatact gagggtttcc gggtcctctg cgagcgattc tggctgttcg
     4321 gcagctgcat cgagagtcac aggaagtagc tgggggctct ctgaagaagg taatagcagc
     4381 tttccctcgc gccattgttg tcgccatttg aacaacagat tggcgttaat gccattttca
     4441 agagcaagtt ttgagatgga tatcccgggt tcacaggagg cagcaacgag cagctgttta
     4501 aattcgggag gataattagg gcagcctttt cgcctgccgg gagtcacatt tttctgcata
     4561 tctgacactt tggttcccac tacttatttg gtggacacca ctttgtctaa ttcgtcagat
     4621 tctgaccaga cggttcaggc tgtacgctta ccactatttt atccctgttg aatttggtta
     4681 agccggtgca gacgcgccgg gaaacgcaca cctgaaccgg cacaataaaa tacaatacaa
     4741 ccccctgtta ctggagatac gaatggaatg acgaggcagc ctgtcagtca cagggggctg
     4801 ttggcataaa atcaacggaa aggccaccaa caccggaatt aagctccaca gtccccgcac
     4861 tggcaatcca ttcccgggaa aggcatcacc gcaaactgct ccggaggcaa ccattacagc
     4921 ctctgtaacg gattatcagc cctggcatct tccgacggca cctcctgccg cccccacgcc
     4981 ctgctgtata ccccggtacc gcagcgtggg cagcactttt ccccctcata attttcgtca
     5041 cacatcacac aaaaccactt tctcaccttc accgtataat ccggaaccgt catgccctgc
     5101 agatgcaaac gacgggcctc tttctctgcc gggtcaagcc aggtacatcg cagcaggatt
     5161 ttttcagcga cagtcatctg gtcatgttca aaccacggtg gttgtccgtc attgccccgc
     5221 tgaagtgtta acgggcatcc acaggacaca cagcacaaaa cgtcctgtcg gttgtgcttc
     5281 gcagcttctt tcgccgtaac aaaatggcct ttgctgtcat tcgccatata acagttcaga
     5341 attcgcatat ttcctcctga gttgtccggg gaatactgaa tcctccggtc tggcagtgtg
     5401 tgcagtactg ctccccataa taatcgtggt gacattgtct gcagtgccag ctggctttac
     5461 gcaccacggg taaggcatcc ggtacgaatt gctgcagacg tttaatcaac tgtatttctc
     5521 tgcgctccgg cctgacataa gggcactgtt gaccgtgctc tgtcagcctg tcgtcagtgt
     5581 gttcaaacca gggaagttca gtgtcgtatt gcggatggta tctgagtgca ctgccacaca
     5641 agtggcaggt gtagttagca taaggtgcgg cctgtgcagt acgggcgccc gtcagacgtc
     5701 cgttgccatc aagagcgata aaggattttg cgtacatagt catatacctc agccgccaga
     5761 cgacacgcag gcaacagccg tcccggtacg ggcagcgtgg tcagggtgtg aatggcggtt
     5821 cagttaaaat ctggactgga gtttgggtaa agcaggctgg ttgctgttac agcactgtgg
     5881 ttgtcacaca atggaacact gacgtaaaaa acagtcagtg ggcgagtgga tacatcgttg
     5941 ccgccagaca acaacccata cttttggttt gccggatatt ggcgcgccag tcgtgctcaa
     6001 ctccggctac agccaaaatt gcgctcaccg gtgcagtgag ctttggtatg ctccttcgcc
     6061 agatagtcag cacgttccag cacttgctga aagccggtgt catcattacg cgccagccac
     6121 accgccggcc aggccggaaa atgcaccagc gtggcatggc acccgacatc cactcccagg
     6181 gcactgcacc acgcctgttt aatcatcccg gcgagagact ccggagcgcg gtaatcaccg
     6241 gcacgacacc aggtatcccg gttgaccagc agcatcaggt gatagtgttt tttacctttg
     6301 cactccccaa actcccgggc ccagacgtaa tgcagggttg tgggatgcac gcgtttacct
     6361 tcccgatgct tacgtttctg gtaagcgtcg attcgggctt tcagggcatt gatgaagcgg
     6421 gatatcacag ctgcgtccgt tgctgccggc acatccggga gacgcagatc aacccgaagt
     6481 gccgtcaggc ggggatgaac atccagtgcg tgccgcaccg tctcacgaat acgttgctgc
     6541 cagaaggggt tgtatttgta ggtcatggtt aaatctccgt atggttcata cggaatagcc
     6601 acgtcgtaaa aaatacgcag agtccctgaa gtgacctccg gcaggacact gtctcaggca
     6661 ctctgtgata acccgaacgg tcgtctccgg actgacggtt gaattttcag cgttgttttt
     6721 taatgtaaaa aaccggttac gggtaaggct gtgaggaggg agtgatggtg atacgcaaaa
     6781 agaagtgcag ggactgtgga aacgcgatta ctcataatac agtgtgttgc ccgtattgtg
     6841 gttctgtcga ccccttcggc tactaccgga atacagaccg tatcgttacc atactgctgg
     6901 cgctgattat tgtggtcctg ctgactactg tcagcgtcag tgtgtgtata ctgtgtagct
     6961 ggtgagataa cggttcatgt atcccctgag ctgacaatgt accgtgtata ttaagcgtaa
     7021 ccgacaggtt ctccttcata ccatattaaa ttttccaggt attgccgctg ccttatttcg
     7081 tcccatacag gaaaactcat ctgggtttct gacaaatacg cgaaactcac agcccctcaa
     7141 tatcaaccac acgttgtaag ccggtaatac ctgaaaatca gcctattacc gcccacgcct
     7201 ccggcaccat tcccttttga tttcgaattc tttgttgccg ttatactgct taatgttccc
     7261 gatcttccgg tgcacagata acaccggagt catcgtagcg cggattcaag attcaggtga
     7321 catatttcca gacggaagcc ggcacgcgat cgtaaagttt gttcattgtc agttctgcca
     7381 gccggtgatc gaccgcagag ttaaaggcca gttccgcagg ggtaagttta taacgtgtgt
     7441 gggaaatcac tttttccagc gtctcccggg atgaacaacg acggaactga tacagccagt
     7501 cttctttggt tttaacttcc attagcctct catcacttta tgctgcaatt aacgtgatac
     7561 tgccagcata ccgcacctga ctggcattct ggtacccacc ccaaactcat tactcccgca
     7621 ccaccactta accggtctct cagactgtca ctcaccaggt cggatacgtt taccgctcag
     7681 acggatgctt aatgcactct gttcacgtgt gttgcaggta cgttttatat acgcaggtta
     7741 tacgccagct atcagggata gccctgtgag tacaacgtaa tctgttgcta ctgcgttctc
     7801 atacccactg atcacactca cagtgtttat ttcacagata tgaactcatg tgtttgatct
     7861 ctcttatata tttataatac caatcagaca gtcttatccc atcggtgata cctgtctctg
     7921 cccacaaggc acgagctgcc ccaccgtaac cgccccgtaa ataactctgc aattataaga
     7981 acgacggttc ttcccctcat cctcccgaaa atcatccctt ctctgtcgtg cgtacgcgcg
     8041 agaaacatcg tttcgcggta gccaccgggt atcccagcat catgctccct gtttatctcc
     8101 cgacaggaat attcccctgc tcatttttta cgtttctccc cggttgttat gccagtgaag
     8161 gtggtgcgtt tttcatcacc aacgccggtt gagattaaca acatccggag gaacattttc
     8221 atgaataccc cagtttcgct gatggatgac cagatggtcg acatggcgtt tatcactcaa
     8281 ctgaccggct taaccgataa gtggttttac aagctcatca aggatggtgc ctttccggcc
     8341 cccatcaaac tcggccgcag ctcccgatgg ctgaaaagtg aagtggaagc ctggttgcag
     8401 gcgcgtattg cacaatcccg tccgtaattc ctgaccatta cccgttcaac cgcagcgaat
     8461 acactatccg gcctccggag gcattttgct gcgtgctaaa ggagagaata ttatgcaaaa
     8521 acttgaaaac cggtgtgatc tgctgctcac ccagcatcag aaatggatgg caagtgtaac
     8581 ccggtttatt gtcgctcacg gaatgggctc tccgcatctt catggttatc atcgcctgac
     8641 cctggcccac ttttttctgc cggaaaaagg caccatcatc tcggtggcac cacagggact
     8701 ctatcaggta gtcaacccgg caacgccacc gttcattcct gccattcagg aagggttaat
     8761 gaccagcatt cagactcatg aaatcatgtt acttacacat tttaacctgg gtggtgtttt
     8821 actcagtgaa ctacatcgtc tgggagaaag ccgactggca aacaggctga acagtctgct
     8881 ccggcgtttt gacgatcgcg atctctacca caccctgata tggctgtgct ggtatgatct
     8941 gatgtgcgcc cactccatgc agccctggac tgaagagtta aagcataaat cacacgcaga
     9001 acttgaaagc tgggctgtgg cgcgcaaacg ggaaaagcgc gagcttgaac tgatgattga
     9061 cgagtacctg ttatacgcct gttgattcat aatcactccc gggcggtacg tagtcagtga
     9121 taccggaatt tccggagagg gtcgcaggta tattgtttct gtgcaattca tggttattca
     9181 cgttggatgt agtgattgct gcatccaatg ctatcggaaa gagattaacc agcaaatggt
     9241 tcctcaaatc gtattgatat ttacccggcg cattgagcac caccatctgc ccccgttccc
     9301 acatcaaacg gctggaccgg aaaacgattt gcttttcatg ctccgtggaa attcacgaaa
     9361 aagttattgg gtcattcatc gaaaaaaaca tattctgctg actggagtca tcacccagaa
     9421 ataaacgtca actgaacaat aacgattttt tctttgtggg attccataca cttttccatc
     9481 cccctctgaa aattagtcat caactcacca acacatcttc tgaaatttgt attaaactta
     9541 ttgcttaaat agatgatata atatctcttc cattgcacgg aatcagcaaa aactgtcatt
     9601 ctggcaatct taacaagaac atttatcggt taattttatc agtaaacaat ggaatatcat
     9661 actgataaaa tgaacattca gggtaagatg atgaaattca catgaaaaat gctgaagatt
     9721 atttatttca tgcagataaa cgataaaaaa caagagagta tatttttcag tgtaagagtc
     9781 aactgttcat ctccactgac aatcacggtc agcatacaaa caaagtgagt cctcctgctt
     9841 agtcattgcg tatcagaaac taaaaaacaa aagattctgc aaacaacgtg acatttttat
     9901 gacatttccc gaatacatgt atatcaataa acggtagtca tcgttgaatt aataagtctt
     9961 acagagctat acgtagctta taaaaccact caacgaccat catcacctga ctatttagcg
    10021 ggagtatcgt ctgtataaat ccataaataa taacacctgt tattaaaata agaatgctgt
    10081 cagccgtaaa ctattaacac aacacctgtc cactctatct gttcttttaa ctcaaccggc
    10141 gtcgaaaata tttcctgatt agtgcatcat agtcttcttt tttctttctc aaccatcaaa
    10201 ccaaacacaa cattcacaac cacacaataa aacaaaacca aaacataaga ttaacaatgc
    10261 cagtcaacag gaaaacactg aatattgagg tgcgctgtaa cctggacagc gcatacggcg
    10321 catgtgatca acacagccca ttcttttctg tcatccccca aatgcagaac aaacaatcat
    10381 aaccaaatga atataaaaga aaatatgatt tttcaccatc ataaaaacat caatgtatct
    10441 ttatataaaa acactgataa catgacatag acatttaaaa acataaacaa atcacaacaa
    10501 aaacatttga taatcactta gtgataaaat aaaactgaga cgattttgtt ataagagagg
    10561 cgacatggag tgagataatg aataacgaac agatgcttat caaatgcaaa gatccccgta
    10621 aatgtcggtt ttatgccgaa tatctgaatc acaaataccc caatgaattc cctcagtttg
    10681 cgcctgacaa cccacaaaac tgtatcagaa atgttaattc gtttttaaaa gaaaagtatt
    10741 ataaagcttc aaacaattct tttggtgttt ttggttcgac tgcagaagaa gctctcagca
    10801 aagaaattaa tgaacttgaa attattgcag ggcaggcaat actgcctgaa gagagttttc
    10861 tctggttgaa aactgacgaa cttgcgactt tctttacctg gactactatt tatctgtcga
    10921 aggaagacca tacaaaattc ccgaaaataa accataattc atcaggaata aaaaacggaa
    10981 ttgaagtcag gaaaatacga acagttaccg atgaaacgtt aaatacttac aaaaaaagta
    11041 accttccgga taatccatca tcccataacg aacgaataaa agtaatcatt tcatattttg
    11101 actcctgcca ttctttaatt aataaaaatg caaaaagcat atacctcgag gaactcagac
    11161 aggcgtggct taaaaagaaa agcgcttgtc aaaagtttaa gtggctcctt cctgacgatg
    11221 atgaaatgtg tcagtggttc tggacaagac tgaaacaaaa acaggatgaa aaacatccag
    11281 tacataaccc agtgacccgg tggttcatac catcttcaac gaaagaatat tatcttgcct
    11341 ctcttgtcgc ctttgcttta tggaaagaag cgcctgatac cattgaactg ttcagaacac
    11401 gaataaatca tgcctggcat cagaaaaaac aaaaggacaa atatcggagt gatggtaaaa
    11461 aggccattaa tatacatata agaagcaatg tgaaagaaat gcttgatgag ctcagaactg
    11521 tttacggtct gagaacagga gagttttttg aaatgctgat tacagagaaa tatcttgaac
    11581 acaagcgaag gaagtaattc attacctgtt acattctaaa taatctcaga cagatattat
    11641 ctccgtgaat cccccgccac ctttccggtg cgcggggttt tgtctttttt caccgggaat
    11701 acatgtatga atccgtctga tgccattgag gcaattgaaa aaccgctctc ctccctgcct
    11761 tactcacttt cccgtcacat cctggaacat ctgcgcaaac tcaccagtca cgaacccgtg
    11821 attggcatta tgggtaaaag cggggccggt aaatcttcac tctgtaatgc actgtttcag
    11881 ggggaggtca caccggtcag tgatgttcac gccggcaccc gggaagtgca gcgcttccgt
    11941 ctgagtggtc acggtcacag catggttatc actgacctgc ccggggtggg cgagagccgg
    12001 gacagggatg cagagtatga agccctgtac cgtgacattc tgcctgaact ggacctggtg
    12061 ctgtggctga ttaaagccga tgaccgggcc ctgtctgtgg atgagtattt ctggcgacac
    12121 atcctgcagt gcggacatca gcaggtgctg tttgtggtga cacaggccga caaaacggag
    12181 ccctgccatg aatgggatat ggccggcatt cagccctctc ccgcacaggc acagaacatt
    12241 cgcgaaaaaa cggaggcggt attccgtctg ttccggcctg tacatccggt tgtggccgta
    12301 tcggcccgca ccggctggga actggatacg ctggtcagtg cgctcatgac agcgcttccc
    12361 gaccatgccg ccagtcccct gatgacccgc ctgcaggacg agctgcgcac ggaatctgtc
    12421 cgcgctcagg cccgtgagca gtttaccggt gcggtggacc ggatatttga cacagcggag
    12481 agcgtctgtg ttgcctctgt tgcacgcacg gttctgcgtg ccgtccgtga cacagtggtc
    12541 tctgttgccc gcgcggtatg gaactggatc ttcttctgaa cctgttgtgg atgatgtcct
    12601 ccctgcctct gagtctgctc acaaaagcgc tgttttcgtt acggtctctc ttgtccgtgc
    12661 aatagctcaa taatagaata aaacgatcga tatctatttt atcgatcgtt tatatcgatc
    12721 gataagctaa taataacctt tgtcagtaac atgcacagat acgtacagaa agacattcag
    12781 ggaacaacag agccacacgt cagaaacttc cgtcagccgg acctccggca ctgtaaccct
    12841 ttacctgccg gtatccacat ctgtggatac cggctttttt attcaccctc actctgatta
    12901 aggaaatgct gatgaaacga catctgaata cctgctacag gctggtatgg aatcacatta
    12961 cgggcgcttt cgtggttgcc tccgaactgg cccgcacacg gggtaaacgt ggcggtgtgg
    13021 cggttgcact gtctcttgcc gcggtcacgt cactcccggt gctgtctgct gacatcgttg
    13081 tgcacccggg tgaaacagtg aatggcggaa cactggtaaa ccatgacaac cagtttgtat
    13141 ccggaacagc tgatggcgtg actgtcagta ccgggcttga gctggggccg gacagtgacg
    13201 aaaacaccgg cgggcaatgg ataaaagcgg gtggcacagg cagaaacacc actgtcaccg
    13261 caaatggtcg tcagattgtg caggcaggag gaactgccag tgatacggtt attcgtgatg
    13321 gcggagggca gagccttaac ggactggcgg tgaacaccac gctggataac agaggtgagc
    13381 agtgggtaca cgggggaggg aaagctgccg gtacaattat taaccaggat ggttaccaga
    13441 ccataaaaca tggcggactg gcaaccggaa ccatcgtcaa caccggtgca gaaggtggtc
    13501 cggagtctga aaatgtgtcc actggtcaga tggtcggggg gactgctgaa tccaccacca
    13561 tcaacaacaa tggccgacag gttatctggt cttcgggggt atcccgggac actctcattt
    13621 acacaggcgg tgaccagacg gtacacggag aggcacataa tacccggctg gagggaggaa
    13681 accagtatgt gcacaaatac ggacttgcgc tgaacacggt aattaacgag gggggctggc
    13741 aggttgttaa ggcaggtggt actgccggta acaccaccat aaatcagaac ggcgagctga
    13801 aggttcatgc cggcggagaa gcgtctgatg tcacccagaa cacgggcggt gcactggtta
    13861 ccagtacagc tgcaaccgtc accggcacaa accgtctggg agcattctcc gttgtggcgg
    13921 gtaaggctga taatgtcgta ctggaaaatg gcggacgtct ggatgtgctg agcggacaca
    13981 cagccactaa tacccgtgtg gatgatggcg gaacgctgga tgtccgcaac ggtggtgccg
    14041 ccaccaccgt atccatgggg aatggcggtg tactgctggc cgattccggg gccgctgtca
    14101 gtggtacccg gagcgacgga acggcattcc gtatcggagg cggtcaggcg gatgccctga
    14161 tgctggaaaa aggcagttca ttcacgctga acgccggtga tacggccacg gataccacgg
    14221 taaatggcgg actgttcact gccagagggg gctcgctggc gggcaccacc acgctgaata
    14281 acggtgccac gcttaccctt tccgggaaaa cggtgaataa cgataccctg accatccgtg
    14341 aaggtgatgc actcctgcag ggaggcgctc ttaccggtaa cggcagggtg gaaaaatcag
    14401 gaagtggcac actcactgtc agcaacacca cactcaccca gaaagccgtc aacctgaatg
    14461 aaggcacgct gacgctgaac aacagtaccg tcaccacgga tgtcatcgct cagcgcggca
    14521 cggccctgaa gctgaccggc agcacagtgc tgaacggtgc cattgacccc acgaatgtca
    14581 ccctgacatc cggtgccacc tggaatatcc ccgataacgc cacggttcag tcggtggtgg
    14641 atgacctcag ccatgccgga cagattcatt tcacctccgc ccgcacagag aagttcgttc
    14701 cgacaactct gcaggtgaaa aacctgaacg gacagaatgg caccatcagc ctgcgtgtac
    14761 gcccggagat ggcgcagaac aatgctgaca gactggtcat tgacggtggc agggcaaccg
    14821 gaaaaaccat cctgaatctg gtgaacgccg gcaacagtgg tacggggctg gcgaccaccg
    14881 gtaagggtat tcaggtggtt gaggccatta acggtgccac cacggaggaa ggggcttttg
    14941 tccaggggaa tatgctgcag gccggggcct ttaactacac cctcaaccgg gacagtgatg
    15001 agagctggta tctgcgcagt gaagaacgtt atcgtgctga agtccccctg tacgcctcca
    15061 tgctgacaca ggcaatggac tatgaccgga ttctggcagg ctcacgcagc catcagaccg
    15121 gtgtaaacgg tgaaaataac agcgtccgtc tcagcattca gggcggccat ctcggtcacg
    15181 ataacaacgg cgatattgcc cgtggggcca cgccggaaag cagcggcagc tatggcttcg
    15241 tccgcctgga gggtgacctg ctcagaacag aggtcgccgg tatgtcactg acgacaggag
    15301 tgtatggtgc tgcaggccat tcttccgttg atgttaagga tgatgacggc tcccgcgccg
    15361 gcacggtccg ggatgatgcc ggcagcctgg gcggatatat gaacctgaca cacacctcct
    15421 ccggcctgtg ggctgacatt gtggcacagg gaacccgcca cagtatgaaa gcgtcatcgg
    15481 gcaataacga cttccgcgca cggggctggg gctggctggg ctcactggaa accggtctgc
    15541 ccttcagtat cactgacaac ctgatgctgg agccacggct gcagtacacc tggcaggggc
    15601 tttcgctgga tgacggtaag gacaacgccg gttatgtgaa gttcgggcat ggcagtgcac
    15661 aacatgtgcg tgccggtttc cgtctgggca gccacaacga tatgaccttt ggtgaaggca
    15721 cctcatcccg tgcccccctg cgtgacagtg caaaacacag tgtgcgtgaa ctgccggtga
    15781 actggtgggt acagccttcc gttatccgca ccttcagctc ccggggtgat atgcgtgtgg
    15841 ggacctccac cgcaggcagc gggatgacgt tctctccctc acagaatggc acttcactgg
    15901 atctgcaggc cggactggaa gcccgtgtcc gggaaaatat caccctgggc gttcaggccg
    15961 gttatgccca cagcatcaac ggcagcagcg ctgaaggtta tagcagtcag gccacgctga
    16021 atgtgacctt ctgataattc tgcattgcct ctctgtggtc ccggtcatca tgaccgggac
    16081 ccggacgggg gaaaacgttt cagtgccaca ttcactggca ttcacaataa catgatattc
    16141 atcacggagt gactatgtta cagatagtcg gcgcgctgat tctgctgatc gcaggatttg
    16201 ccattcttcg ccttttgttc agagcattaa ccagcacagc gtctgcgctg gcagggttcg
    16261 tattgctgtg tctgttcggc ccggctttac tggctggcta tatcactgaa cgcataaccc
    16321 ggttattcca tattcgctgg ctggcaggcg tatttctgac gattgccgga atgattatca
    16381 gcttcatgtg gggacttgat ggtaaacata tcgcactgga ggctcatact tttgactctg
    16441 taaaatttat tctgaccacc gctctcgccg ctggtctgct ggctcttccc gtgcagataa
    16501 gaaccattca gcagaacggg ctcacacctg aagatatcag caaggaaatt aacgggtatt
    16561 actgctgttt ttatactgct tttttcctta tggcgtgttc tgcatacgca ccattgatcg
    16621 cattgcagtt cgatatttca ccctcactga tgtggtgggg cgggttgttg tactggctgg
    16681 ctgcattagt ggcgctgcta tgggcggcca gccagatcca ggcgctgaaa agactgacca
    16741 gtgccatccg ccagacactg gaagaacaac cggtgctcaa cagtaaatcg tggctgagca
    16801 gtttgcaaaa cgattacagc cttcctgaaa cgctgacgga gtgcatctgg ctgacactca
    16861 tttcacaacg gatttcccgg ggagaactga gggaatttga actggcagac ggaaactggc
    16921 tactggacaa tgcctggtat gaaagaaaca tggcgggttt caacgaaaag ctgagagaga
    16981 gcctgtcatt tacccctgat gaactgaaaa ccctcttccg gaaccgcctg aatttatcac
    17041 cggaagcgaa tgacgatttt ctcgatcgtt gcctggacgg cggtgactgg tacccctttt
    17101 cagaaggccg ccgttttgta tcattccacc acgtggatga gcttcgtatc tgtgcctcct
    17161 gcgggctgac agaagtacat catgccccgg aaaatcataa gccggatccg gaatggtact
    17221 gctcctctct ttgtcgcgaa acagaaacac tgtgtcagga catttatgaa cgttcttaca
    17281 ccggttttat ttccgatgca acggcgaatg gtctgattct catgaaactg ccggaaacct
    17341 ggagtacaaa tgagaaaatg tttgcttccg gagggcaggg acatgggttt gccgctgaac
    17401 ggggaaacca tattgtcgac agagtccgtc tgaaaaacgc acggatcctc ggtgataata
    17461 atgccagaaa tggagcagac agactggtca gcggaacaga aatccagacg aaatattgtt
    17521 caactgcagc ccgtagcgtc ggtgcggcat tcgacggaca aaacggacag tatcgttaca
    17581 tgggaaatca tggtcccatg caactggaag tcccccgtga tcagtatgcc ggcgctgtgg
    17641 aaaccatgaa gaataagatc cgcgaaggta aagtacccgg tgtaaccgat cccgcagaag
    17701 cgtcccggct gattcgtcgg ggacatctga cttataccca ggcccgtaat atcacccggt
    17761 tcgggaccat cgaatcggtc acttatgata ttgccgaggg gtcggttgtc agtctggcgg
    17821 ccggagggat cagttttgcc ctgacggcat cggtcttctg gctcagcacc ggcgatcgcg
    17881 atgctgccct gcagacagct gctgtccagg caggaaaaac cttcacccgc acactggctg
    17941 tctacgtcac aacccagcaa cttcaccggc tcagtgttgt tcagggtatg ctgaagcata
    18001 ttgatttttc gacggccagc ccgactgtcc ggcaggcgct tcagaagggg accggtgcag
    18061 gaaatatcag tgccctgaac aaagtgatga agggtacgct ggtgacatct ctggcactgg
    18121 tagctgtcac aaccggccct gacatgatca aaatgttgcg gggacggatc tccggtgcgc
    18181 agttcatcag gaatcttgcc gtggcatctt ccggtgtggc aggtggtgct gtcgggtcag
    18241 tggcgggcgg gatattgttc agtccactgg gaccatttgg tgcactgaca gggcgtgtgg
    18301 ttggcggtgt tctgggggga atgattgcct ccgctgtatc aggaaaaatt gccggagcgc
    18361 tggttgaaga agatcgcgtc aaaattctgg caatgattca ggagcaggtg acatggcttg
    18421 ccggcagttt cctgctgacc ggacatgaga ttgaaaatct gaacgcgaat ctggcccgtg
    18481 ttatcgatca gaatgctctg gagatcattt tcgccgccgg tatacaacaa cgggccgcga
    18541 ccaatatgtt aatcaaacca ctggtggtca gtatcatcag gcaacgcccc gtcatggaat
    18601 atgatgcatc ccatctcggc aatatggtta accgactgga agaagcattc cccccggagt
    18661 taccggcata acggctattc tgcgcctttt acgttttatc acctgatttg tcagtgtcat
    18721 cgtcatgact cagagaggta aataccatga ttcacctgtt caaaacctgc atgattaccg
    18781 ccttcattct ggggttaacg tggtctgccc cactccgggc acaggatcaa cgttacatca
    18841 gtatacgcaa tacagatacg atatggctcc cgggaaatat ttgtgcctac cagttccggc
    18901 tggataatgg cggaaacgat gaaggatttg gccccctcac catcactctg caactcaaag
    18961 acaaatatgg tcagacgctg gtgaccagaa aaatggaaac ggaagccttt ggtgacagta
    19021 atgccacgcg aaccacagac gcatttctgg aaacggagtg cgtggaaaat gtcgccacaa
    19081 ccgaaatcat taaagcaact gaagaaagta acggccatca tgtcagtctg ccgttatcgg
    19141 ttttcgatcc ccaggactac catccactgc tgattaccgt ttccggaaaa aacgttaact
    19201 gatccctgaa gtcagtttca tgcttcagcc tctgtcacaa cgcgtggcag aggcttttta
    19261 tttatcagga gaaaacagat atgccgggtt acacagag
//
LOCUS       DAZGJT010000090        19169 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZGJT010000090 DAZGJT010000000
VERSION     DAZGJT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19169
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.090"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..536)
                     /locus_tag="ACHKFK_003536"
     CDS             complement(<1..536)
                     /locus_tag="ACHKFK_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO9053796.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDAL
                     LIELPGEEYTVAAVSCIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKAMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFSMGYQRQVTG"
     gene            complement(586..1284)
                     /gene="yjfJ"
                     /locus_tag="ACHKFK_003537"
     CDS             complement(586..1284)
                     /gene="yjfJ"
                     /locus_tag="ACHKFK_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO9053797.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAATQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(1303..1704)
                     /gene="yjfI"
                     /locus_tag="ACHKFK_003538"
     CDS             complement(1303..1704)
                     /gene="yjfI"
                     /locus_tag="ACHKFK_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFO9053798.1"
                     /translation="MTWNPLALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLRNQKLMPLSSVGISSVQQEEYYIVFGALS
                     LKSSLEDILLEITSLVDNALDLAEITEEYSH"
     gene            complement(1831..2562)
                     /gene="rlmB"
                     /locus_tag="ACHKFK_003539"
     CDS             complement(1831..2562)
                     /gene="rlmB"
                     /locus_tag="ACHKFK_003539"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO9053799.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(2653..5094)
                     /gene="rnr"
                     /locus_tag="ACHKFK_003540"
     CDS             complement(2653..5094)
                     /gene="rnr"
                     /locus_tag="ACHKFK_003540"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO9053800.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAAEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGIVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKVGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVSDWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKPKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(5133..5558)
                     /gene="nsrR"
                     /locus_tag="ACHKFK_003541"
     CDS             complement(5133..5558)
                     /gene="nsrR"
                     /locus_tag="ACHKFK_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO9053801.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(5763..7061)
                     /gene="purA"
                     /locus_tag="ACHKFK_003542"
     CDS             complement(5763..7061)
                     /gene="purA"
                     /locus_tag="ACHKFK_003542"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO9053802.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(7165..7362)
                     /gene="yjeT"
                     /locus_tag="ACHKFK_003543"
     CDS             complement(7165..7362)
                     /gene="yjeT"
                     /locus_tag="ACHKFK_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO9053803.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(7444..8448)
                     /gene="hflC"
                     /locus_tag="ACHKFK_003544"
     CDS             complement(7444..8448)
                     /gene="hflC"
                     /locus_tag="ACHKFK_003544"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO9053804.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTSAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(8451..9710)
                     /gene="hflK"
                     /locus_tag="ACHKFK_003545"
     CDS             complement(8451..9710)
                     /gene="hflK"
                     /locus_tag="ACHKFK_003545"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO9053805.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPSGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTDPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(9796..11076)
                     /gene="hflX"
                     /locus_tag="ACHKFK_003546"
     CDS             complement(9796..11076)
                     /gene="hflX"
                     /locus_tag="ACHKFK_003546"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO9053806.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLDDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(11152..11460)
                     /gene="hfq"
                     /locus_tag="ACHKFK_003547"
     CDS             complement(11152..11460)
                     /gene="hfq"
                     /locus_tag="ACHKFK_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO9053807.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(11546..12496)
                     /gene="miaA"
                     /locus_tag="ACHKFK_003548"
     CDS             complement(11546..12496)
                     /gene="miaA"
                     /locus_tag="ACHKFK_003548"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO9053808.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHLLLNIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEIDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(12489..14336)
                     /gene="mutL"
                     /locus_tag="ACHKFK_003549"
     CDS             complement(12489..14336)
                     /gene="mutL"
                     /locus_tag="ACHKFK_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO9053809.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGANQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAVREPVAPRYSPAPASGRRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEVPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(14346..15674)
                     /gene="amiB"
                     /locus_tag="ACHKFK_003550"
     CDS             complement(14346..15674)
                     /gene="amiB"
                     /locus_tag="ACHKFK_003550"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO9053810.1"
                     /translation="MMYRIINWLVATLLLLCAPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPVVAKRVETPAVVAPRVSEPARNPFKT
                     ESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNVTIA
                     IARKLRTLLNNDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNRSAT
                     GASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQFGHS
                     QRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEERLL
                     ASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(15693..16154)
                     /gene="tsaE"
                     /locus_tag="ACHKFK_003551"
     CDS             complement(15693..16154)
                     /gene="tsaE"
                     /locus_tag="ACHKFK_003551"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO9053811.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(16126..17673)
                     /gene="nnr"
                     /locus_tag="ACHKFK_003552"
     CDS             complement(16126..17673)
                     /gene="nnr"
                     /locus_tag="ACHKFK_003552"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO9053812.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLVKAIGIDVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANTH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSQWLIPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCTQRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKMSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            17672..18811
                     /gene="queG"
                     /locus_tag="ACHKFK_003553"
     CDS             17672..18811
                     /gene="queG"
                     /locus_tag="ACHKFK_003553"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO9053813.1"
                     /translation="MSEPLDLNQLAQNIKQWGLELGFQQVGITDTDLSASEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPCKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHITWAIAQQIERRNACVVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(18794..18847)
                     /locus_tag="ACHKFK_003554"
     CDS             complement(18794..18847)
                     /locus_tag="ACHKFK_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053814.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(19081..19156)
                     /locus_tag="ACHKFK_003555"
     tRNA            complement(19081..19156)
                     /locus_tag="ACHKFK_003555"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19121..19123),aa:Gly,seq:gcc)
BASE COUNT         4411 a         5403 c         4716 g         4639 t
ORIGIN      
        1 tctccggtca cctgccgttg gtagcccatg ctaaaattat gcacttccca tttggcatgg
       61 ttttgatttt ctactttttc cagcatgtag acgggttcga tatttcccgg ttcttcgctg
      121 ttaaaaaatc gctgccagcg tttttcctgc cagtttaagg tcattgcccc catcgctttg
      181 gcgttgatcg cctcgcgcca gtgactctct ttactgatac cgtagctctc ttcatagaca
      241 aagagcttga tatcatcaat gtcatcaata tcttcgccgc cagtggtatt gatttgcagg
      301 aactcatcgc ccgaagtgta gtaacggaaa atctggctac ccccgcccag atcgatgcaa
      361 ctgacggcgg ccacggtata ttcttcaccc ggtagctcga tcagcaatgc atcttccagc
      421 aaacgaaacg ctaacgtatc gagcgtaaaa ccactattga gatgcagacc caacggaccg
      481 cgagcgatag caggcttatt atccttgcca aacagacgct ggaaaaaacc agacatattt
      541 tatccttaaa atagttgcca gccttgcagt gaggctggca aaaaattact cgccctgttg
      601 gcgttgcagt cttgctaata catcctgggc gctactttta ttgctaccgc caattccggc
      661 ttccgccagc ttttcatcga gatcgcgacc gtctgcgact ttttccaact gtgtggcagc
      721 atccaggcga gcctgacgtt cggcctgacg cgtttgcagg cgttttaagg attctgccgc
      781 tgtcgaaacg ctggaagatg cgccaacagt agaggtcgtt acagcctgtt gtgcacgctg
      841 catggcttca gtggctttaa cgacttccat ttgctgctca aactgagcga tacgctgcgc
      901 tgtcgctgta actgcttttt ccacgccatc acgagagact tccagattcg acaacacttg
      961 ctcttcagcg gtaatgagat tctcaaggcg cgcgatttct tcagcaactt cgttaatcaa
     1021 cgacggattc acgttcttgc ttaacgcttc cagcgcacga gcttccagac tggctttgcg
     1081 ctcacgtaaa tctttcagct tatcgtgact taatttcact cgcgccagca gatcaacgcg
     1141 agattttccg gctttatcga gttcagcttt agcgtcacga atatgctgtt ccagcatgcg
     1201 cacgccctgg gtttcttcaa tggactcttc cgcctgggag ataaacgatt tccccagcgt
     1261 aaataaactt tttaaaattc ccataaatac ccgtccttgt aattagtgtg aatattcttc
     1321 tgtaatttca gccagatcca atgcgttatc taccagcgag gttatctcaa gcaggatatc
     1381 ttcaagagaa gatttaagcg ataacgcacc gaaaacaatg taatactctt cctgttgcac
     1441 gctggagatc cctaccgatg acaatggcat cagcttctga tttcttaata agaaggtatt
     1501 aaattcatct ggattgctga tactacttac cggacaaata aaggtttcga tgatcatttg
     1561 gcgggaagta aaaagaatat tgatttgcaa atcgccataa tcattcattt taataattaa
     1621 tgcgctttcg ctatttgtta catcaatatt ttgttcaggt acagtttgca gcgccgtcgc
     1681 cagcgccaac ggattccatg tcatatatta ttccatatag attaagttta aatattaata
     1741 aaattaatat ttgcagtacg taattatctt accagctata gacaaaaaaa aaccatccaa
     1801 atctggatgg cttttcataa ttcagaggaa ctagctgcgc tgacgaaccg cttcaaataa
     1861 gcaaattccg gttgcaaccg aaacgttcag ggaagaaaca cttcctgcca ttgggatgct
     1921 gatcaactca tcgcaatgtt cacgggtcag gcgacgcata ccttcacctt ccgcgcccat
     1981 caccagcgcc aggcgtccgg tcattttgct ctgatagagt gtatgatccg cctcgcctgc
     2041 cgtaccgacg atccagatat tctcttcctg caacatacgc atggtgcgcg ccagattagt
     2101 cacccgaatc agcggaacgc tttctgccgc accacaggcc acttttttcg ccgtggcgtt
     2161 gagctgtgcg gagcgatctt tcggcacaat caccgcatga acaccagcgg cgtccgcgct
     2221 acgcagacac gcgccgaggt tgtgcggatc ggttacgcca tcgaggatca gcaggaacgg
     2281 ttgatcgagc gaagcgatca gatccggcag atcgttttcc tgatactgcc gtcctggctt
     2341 cacacgagcg ataatgccct gatgcacggc accgtcgctt ttctcatcga gatattggcg
     2401 gtttgccaac tggataacca cgccctgaga ctcaagggcg tgaatcagcg gtaacagacg
     2461 tttatcttca cggcctttta aaataaagac ttcctgaaaa cgttcagggg cgcgctccag
     2521 cagggcctgc actgcatgga tgccgtaaat catttcgctc attaatgtac tcgttgttta
     2581 cgttttgccg ggagcgccac gcttgcccgg cctacggata attcaccctc ttctgtttga
     2641 agagggtaat gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc
     2701 ggctattttc tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc
     2761 tttcggcttc ggttttttct cgccgcgaaa ggcgctgtct ggctcaaagt tgaccttttt
     2821 gccaacctga cgccgcttgc cgcctttttt gcctgcatcg ccttttttcg ctttctcgcg
     2881 cgccgtttta ccgacattgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt
     2941 gcgctcgtcc atattaaccg cttcaacgcg aacttccacg cgatcgccca ggcgataagt
     3001 ctggccgctg gattcaccca tcaggcgttg ccctacctga tcaaagcgat agtagtcatt
     3061 gtccagcgaa gagacatgga ccagaccatc aatgaacaag tcgtccagac ggacgaagaa
     3121 gccaaagcca gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat
     3181 gaagtcacac ttcagccagt cagacacatc acgcgttgct tcatcggcac gacgttccgc
     3241 catcgaacag tgctgaccca gttgcagcat ctcttccatc gaataatggt agccgccggt
     3301 ttcagtggtg ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg
     3361 caacgtcagg tcaggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc
     3421 cagaccgaag tgaccacggt tttccggatc gtaaatcgcc tgtttcatcg agcgcaaaag
     3481 catggtttgc agcatttctg catcaggacg atcggcaacc gattccagta actctgcata
     3541 gtcgcgcggt tccggcttgt taccgcccgg cagttccagc cccagctccg ccagcactga
     3601 acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca gtgccggttc
     3661 tttggctttc tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat
     3721 taacttgtgg gcgtcgttac gctgagtctg ttcgatacgt tcaatacggc gttcagcgtt
     3781 gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca ccgcgttctt cacgggcttt
     3841 atccagcact ttatagaggt tatgtaactc ttcgagatgc ttaaccagcg gcgcatactg
     3901 ctcgcgcaga tcctgatcgc cttgcagtat atgccagact ttggtgtagg tcagacgcgc
     3961 gtgagaactc atcaccgctt cgtagaattt gtagccagtc aggcggcctt tcgacgaaac
     4021 cgtcatctcg cacaccatac acaggcggtc tacctgcggg ttgagcgagc acaggccgtt
     4081 agagagcact tccggcagca tcgggataac ctgcgaaggg aagtacaccg acgtaccacg
     4141 attacgcgct tctctgtcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat
     4201 cgcgacccat aaacgccagc cgccgccgcg ttttttctca cagtaaactg catcgtcaaa
     4261 gtcacgggcg tcttcgccat caatggtgac cagcggtaaa tcgcgcagat cgacacggcc
     4321 cacttttgct tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg
     4381 cggccagatg tacggaattt catgggtacg cagagcaata tcaaccgcca tgccggtgcc
     4441 catattatcg cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg
     4501 ttgagtcagt tcaaccacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg
     4561 gattaagata tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc
     4621 ggtgaagtag cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc
     4681 ttcacgacga cctttacggt cagcgcccag cggctgagcc agcacctgat cgccatgaat
     4741 gcaggttttc atctgctcgc tggagagata caaatcatct ttacgccctt caactcgcag
     4801 aaagccgtag ccatcacggt ggccaataac gatacctttc accaggtcga ggcgttccgg
     4861 cagcgcatag cactgacggc gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag
     4921 gcggcgacgc aggccttcaa gctgctcttc gccttcaatg tgcagttctg ccgccagctc
     4981 atcacggctg gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg
     5041 gatgggattc gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt
     5101 tcctccgttg tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc
     5161 ggttgattct cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt
     5221 tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca
     5281 ctgctgcaat tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatt
     5341 gcactcgccg gtttacccaa gcgaatgccg ccattttttc cacgaacagc agtcacgtag
     5401 ccggcacgac taagttgatt gattattttg accatatgat tacgggagac gccgtagacg
     5461 tcagtcactt cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc
     5521 gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg
     5581 gggaaaaata catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc
     5641 agttcgggga aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac
     5701 cgacacagca atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag
     5761 aattacgcgt cgaacgggtc gcgcagaatc atggtttcag tacgatccgg accggtagag
     5821 atgatatcga tcggcacacc ggtcagctct tcaatgcgct tgatgtagtt cagtgccgcc
     5881 tgcggcaggc cactacgatc tttcacgccg aaggtggatt cagaccagcc cggcatggtt
     5941 tcgtaaatcg gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttca
     6001 cgaccatccg gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg
     6061 tccagtttag tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca
     6121 acggtgtcca gccagccggt acgacgacga cgacccgtag ttgcgccgaa ttcgttaccc
     6181 tgcttgcaga ggaactcgcc cgtttcatca aacagttcgg tcgggaacgg acctgcaccc
     6241 acacgagtgg agtaagcttt gaggataccc aaaacgtaat caacataacg cgggcccagg
     6301 ccggaaccgg tcgccacgcc accagcagta gtgttggaag aagttacgta cggataagtg
     6361 ccgtggtcga tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc
     6421 tgacgcgcct ggtcgagcag atcagaaacg tcaacaacca tagaagtcag gatgtcggca
     6481 acagccatcg tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta
     6541 accaactgga agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg
     6601 tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg
     6661 ataccacgac cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc
     6721 agcgcaacgt gataatcaag gatcagcgga catgcttcgg acagcagcag acgctcacga
     6781 acggggatgc cacggtcttc cagttctttc atctctttca tcagcgcagc cggagacagc
     6841 acaacaccgt taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga
     6901 tggagaacgg ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg
     6961 tagcgtacaa catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg
     7021 tcaccccatt gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg
     7081 cttaaaaatg gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat
     7141 gacctcgatc agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac
     7201 aacgcccgca accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt
     7261 catcgcagag atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag
     7321 taccaaaacc agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa
     7381 aagaaaacca ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt
     7441 atattaacgc gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tgtccgggct
     7501 catgaccatc acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat
     7561 gaatgcgtag aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttctgc
     7621 atcgccttca ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac
     7681 ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg
     7741 acgcgctacc gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt
     7801 cggcaggttg atctgcttaa ttcgcacatc gacaacttca atacccagcg ccgccatact
     7861 gttcggattg atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc
     7921 ggcaatggcg ttatctgctg cagaggtagt aacttcatct tctgtacccg cagaaccgga
     7981 gttcagcgcg tcacgtactt cgagggtcag acgaccacga gaatcggtga cgatgtcttt
     8041 cacgtccagg cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac
     8101 ttccgcttgc gaaatgtcgc caccacccgt tgccagatag taacggctga aatcgctgat
     8161 gcgccatttg atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc
     8221 ggcctggttg tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg
     8281 tatcttgaaa tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac
     8341 cttaccaaaa cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta
     8401 aagcactacc agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc
     8461 tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga
     8521 ctggtggacg acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcagacgc
     8581 agcagattgc tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc
     8641 tggtctaacg gcagaaccat caggttgcca cctttgtcgt taaccagcac tttgcgggtg
     8701 ttacccaaca ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgca
     8761 gctttatatt ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaagatg
     8821 gtctgggcct tgtacgcacg tgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc
     8881 ggctgaactt cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc
     8941 gcggcaatcg catcgtcaaa cgccgctttt acttcctccg gcggacgagc agcctggaag
     9001 ttgacgtcca gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc
     9061 tgggtatcgc tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta
     9121 ccgataaccc cgcgcagggc gctgtcggta gcctgacgca gactgtcatc cgggctggtc
     9181 acgctataca gatatttttc cggatcggtg acgcggtact gcacgttcat ctcaacgcgc
     9241 actacgttct cgtccgacgt cagcatcaca ccagaagctg ccagttcacg cacggcttcc
     9301 acgttcaccg gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc
     9361 agatggctga atttaccaaa gcgtgttacc acgccacgtt cggcttcttt aatggtatag
     9421 aaaccactgg ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg
     9481 ccaagctgcg ggcgtgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg
     9541 ccgcccagac caccgagctt tttgctcagc ttgcggaaga tatcatctaa atcaggtggc
     9601 ccttgatcgc gaccgccttt gtttccattt ccctcagagt tgccgctagg tttgctgctt
     9661 ccccacgggt cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc
     9721 catatttgtt atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct
     9781 tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg
     9841 acgccagtca acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca
     9901 ctctttttct attgcctgaa gctgataaaa acgacttctc aggcgccctt cctgcggcgg
     9961 cagacgcaat gtatgctgcg ccacctcgcc ggaaagacgc tccgtcaaag cctgaaaaag
    10021 ctgtggtatc cccgctccgg tctgcgcaga aagccagaca cggatcggtt tgttctcttc
    10081 atctctgtca atacgcggtt cgaaatcgtc cagcatatcg atcttgttca tcaccagcag
    10141 ggttgggatc tcgtgagcat caatctcttc aagaaccgta ttcaccgctt cgatgttttc
    10201 ttgtacacgc acatccgccg cgtcaatgac gtgcagcagc aatgtggctt gccgcgtctc
    10261 ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc aggtggcgaa taaaccctac
    10321 ggtatctgcc aacacggttt caccgacatc cgcaacgtca atacgccgca acgtcgggtc
    10381 gagggtggca aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag
    10441 agtagattta ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat
    10501 acgcgattgc cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg
    10561 cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg
    10621 caaaccaatc ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc
    10681 cagatggcgc aactgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg
    10741 ggcgaaaata tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgttc
    10801 caggttacgc tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt
    10861 cgctttgaca gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg
    10921 cggcgcttta cggctaccgg taatcacctg caatgcttcg acaccggcgg aagagaccag
    10981 agattcaaac tcctggaggt cttccatatc tttgtcttgc gtaaaataga tgtgtaccag
    11041 taccgcctgc tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca
    11101 gcggggaacg caggatcgct ggctccccgt gtaaaaaaac agcccgaaac cttattcggt
    11161 ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg ctaccatgat gatagttact
    11221 gctggtaccg ccaccggcgt tgttactgtg atgagaaacc gggcgagacg ggacaacagt
    11281 agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt ttcaacagga tcacgaactg
    11341 atcaaaagac tcgatttgtc cttgcagctt aataccattc accaaataaa tagaaactgg
    11401 aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt aaagattgcc ccttagccat
    11461 tctctctttt ccttatatgc ttatttgtac tttgaacctt tcgattctga aaaaattgcg
    11521 cacgatacgt ctcaattgta cacattcagc ctgcgatagc accaacaacc tgtaatactt
    11581 cgtcacgcgc ctgttctggt ttttcactgt caagccagtg aaccccttcc caaccacgca
    11641 gccaggttat ctgccgcttc gccaactgtc tcgtggcgca aacacctcga taaaccattt
    11701 catcgtatga gatttcgcct tcaaggtaag accacatctg gcgataaccc acgcaacgaa
    11761 tggaaggcaa atccgtatgc aaatctcctc gggcaaaaag cgcccggact tctgcttcaa
    11821 aacctgaagc caacatctga tgaaaacgct gctcaattcg ttgatggagc agttcacggc
    11881 tcgccggggc gatggcgaac tgatgcacct gatacggtag agcgtctcct gacgtttgcg
    11941 tcagttccgt taaagtttta cccgaaatga aaaaaacttc cagtgcccgg gaaagccttt
    12001 gtggatcatt tggatgaatc cttgctgccg caaccggatc gatctcctga agttgacgat
    12061 gcaatgactc ccaaccttgc tctgccgcct gttgctcaat tctggcccgt acttccgggt
    12121 ctgccgacgg tagcggcgac aacccttcca gcaatgcctt gaaatacaac atcgtaccgc
    12181 ccactaacag tgggatccgc cccgccgcgg tgatatcggc catttccgcc agcgcatcgc
    12241 ggcgaaaatc agcagccgag taagcctgcg acggatcgcg aatattcagc aataggtgcg
    12301 gcgcggcgag taactcttca gcgttcggct tcgccgtccc gatatccatc cctttgtaaa
    12361 taagggcaga atcaacgctt atcaactcta ctggtaaaat tttacgcagc tcaatggcta
    12421 acgccgtttt accggaggcc gtcggcccca tcaaaaaaat cgccttaggc aggctcgcct
    12481 tactgatatc actcatcttt cagggctttt atcgccggat gtaaatcaac agattgtaac
    12541 agaccacccg gcggcgtttt cacaagttgc ggacataacc gttccacgtc cgccagcagg
    12601 gttatggcct gtgccattga ccactgcgca tgttcgctca tcagatttcg tgcaatccac
    12661 tgcgcaatat tgccaggttc gaatacggac tgcttcgcca ggtagcctat cagttcagga
    12721 atcaagattt gtaaattttg ttggcgtaag ggtaaaggca cggccctgat ggtcacatgc
    12781 tgcgcatcgg actggaaatc aatacccagt tccgccaggg cagactgcgc tttttctaat
    12841 gccgattttt cttcgccaga aacttttagc cgcaacggaa taagcagcgg ttgggcgcaa
    12901 actggcactt cacccggcgt caattgtgcc tgacgcagcc aacgttctgc cactggcaag
    12961 gataaaagtg aaatgttgcc atcgcgctca agcaacgcac agtcggaatg gacgatagtc
    13021 agtacccgac caaaactctg actgttcgcc gcaagtgcag gttcctgcgg ttctggcgct
    13081 tttggttttt gcatcggcgc gggcgtttgc aaaagctggc gatacacttc accttgctgt
    13141 ttctggtaac ctggctgcgc attcggccag ggggcagccg gacgacggcc tgaggcgggc
    13201 gcaggagagt agcgcggagc taccggctca cgaactgccg gttcagcaaa gtgattgcgc
    13261 cccgccgcca cgcgattttc cggaatggca cgcggtgcag gttgtggttc atcgtccagc
    13321 ggtagcggtg tttccagttg ctgttgtagc acgctcagca cgccctgata gataaagtca
    13381 tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg cagggtgcac gttgacgtcc
    13441 acctgatgcg ggtcgatctc cagatacaac acaaatgccg gttgctgatt ggcccccagt
    13501 ttgtcttcgc aggcctggcg gatcgcgtga ttgatcaggc gatcgcgcat catgcgaccg
    13561 ttcacgtagc aatactgaat ttcggccagt gcgggcgtag tgtgatttgg atcggccacc
    13621 cagccgcgca gcgtgagatc gccgtgttgc cattcaatcg ccagcgcttg ttcgagaaaa
    13681 gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc cgccttccgg cactgcgcgg
    13741 tactggcgta caattttgcc gttatgcgac aggttgatag tgacgtcgaa acgcgccagc
    13801 gcaatgcggc ggatgatttc gtcgatatgg ttaaattcgg ttttctcggt gcgcaggaat
    13861 ttgcgccgcg ccggggtgtt gtagaacagg tccagcacct ccagcgtcgt tcccaccgga
    13921 tgcgccgccg gtttaaccgt cacgtccata tcgcgccctt cggcataggc ctgccaggct
    13981 tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg aaaccgaact gatactcgcc
    14041 agcgcctcac cgcgaaagcc cagactgata atggcttcga gatcgtccag agaggcgatt
    14101 ttactggtgg catgacgcgc cagcgccagc gccagctcgt cttttttgat accgcagccg
    14161 ttatcacgaa tgcgaataag tttcgcccca ccgcgttcga tatcaatatc gatacgcgtc
    14221 gcacctgcat cgaggctgtt ttccaccagt tctttgacta ccgacgcagg tcgctcgacc
    14281 acctcacctg cggcaatctg gttcgccagt tgtggcggta agacctgaat tggcatcaat
    14341 cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg tactggccgt ttgtgccgtt
    14401 gcaccctgcg gcgcagattg catcggatgc gcaaggaagt aattgcgcag gcctttgtaa
    14461 atggcttctg ccagctgttg ttggtaatcg tcgctcgcca gcaaacgttc ttcgctgttg
    14521 ttactgataa aaccggtttc gaccagtact gacggaatat ccggcgaacg cagaacgccg
    14581 agactcgcgt gttctggtcg gcgtttatgt atttcgccaa tgcgttgcaa ctgactgatc
    14641 atactggtcg ctacatcata ccctacccgc tgggaatgac cgaactgtaa atccagcacc
    14701 gcctggctta aataggggtc agactgactg ttcgccagca catcgcccgc tccgcccagc
    14761 agctccgact gtttctcgtg ctgctccagc cagctggcca tttcgctgtt ggcgcgacgg
    14821 ttagagagca cccatacgga agcgccagtc gcactgcggt tcggtgctgc atcggcatga
    14881 atcgacacaa ggaaattggc gttttgctta cgtgccacat cactgcgccc catcaccgag
    14941 ataaagtaat ccccgtcacg ggttaaaacg cccttaaaca tcggatcgtt attgagcaaa
    15001 gtacgcaatt tacgcgcgat ggcgatggtg acatttttct cccgcgtacc accggggccg
    15061 atagcgccag ggtcctgacc gccgtgtccg gcatcaatag cgatgataat tttatcgcca
    15121 gtgttagccg tcgcgcgggc tgccggacgc gttaccgtat tactgctgat aacacccgta
    15181 gtgcggttac tttccgtttt aaacggattg cgcgccggtt cgctgacgcg cggtgcgaca
    15241 accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg gcggtggcac atctgcgtta
    15301 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    15361 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    15421 atcgccttca ccagattatt gccgctaaac aacaacggca gcccctgaat tacccccgtt
    15481 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    15541 ccaataaaac tcaacgttat ccgtgcctgt tgattaccgt tagaaacctg aatatcagag
    15601 agcgtcgcgg cccccaccgg cgcgcacagc agcagcaacg tcgctaccaa ccaatttatg
    15661 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    15721 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    15781 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    15841 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    15901 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    15961 agcgtataag tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg
    16021 ctaaaggtgg ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    16081 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    16141 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    16201 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    16261 cgcaccgtgc gcgacacagc ccgcacaggc tgcatcataa gggctcattt tttgcccaag
    16321 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    16381 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    16441 cagcaccgct acgccgccat aacgttgtac cagacgttgg gtgcaatgta agcggtcact
    16501 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    16561 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    16621 caacatcggt ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg
    16681 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    16741 catagtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttctc
    16801 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    16861 acgaatagcc cccgccgtgc cgtgatcgcc accaataatc accagccgcc cgtgatcgcc
    16921 tttatgcgaa gtcgggcgac gcggtatcag ccactgagaa agttgttctg ccgaaaaccg
    16981 ctgaattttc gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag
    17041 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    17101 gatggtgtga tcggcgttga ttaccgcgcc tggcgtagcg ccggtttcag ccagaaggcc
    17161 ggaagggata tcaaccgcca caatcggcgc agggtgagta ttagcgtggt cgattaactg
    17221 gctaatggat tcgcggggcg cttgctgcaa gccggtgccg agcagcgcat caacaatcag
    17281 atctaccgat tcgggccaga caatattcga agcatggatc tcgcctcccg cgtttaacca
    17341 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcactttcct gggccagcag
    17401 cgtgacatct ataccgatcg ctttgaccag ccgcgcgacc acgtagccat cgccgccgtt
    17461 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggataagccg aacgacacac
    17521 ctggaatgcg gcttcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcgcatc
    17581 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    17641 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcaatt
    17701 agcgcaaaat attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    17761 taccgatctc agcgcgtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    17821 cgaaatggac tggatggcgc gtcacggtat gctgcgtgct cgtcctcacg aattattgcc
    17881 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    17941 cagcacgctg aaaaatccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    18001 tcacaagctt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    18061 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    18121 aaaagcaggt cttggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    18181 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    18241 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    18301 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac tggaaggggc
    18361 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    18421 gcttatctgc ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgtg
    18481 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagtgaag agaagttttt
    18541 aaaagtcacg gaaggttcgg cgattcgccg tataggtcac ctgcgttggc tgcgtaatat
    18601 cgccgtagca ttaggcaatg ccccctggga tgaaacgatt ttgacagcgc tggaaagtcg
    18661 taaaggtgag cacccacttc ttgatgagca cataacgtgg gcgattgcgc agcaaatcga
    18721 gagacgaaat gcgtgcgtgg tcgaagtgca attaccgaaa aaacagcgtc tggttcgggt
    18781 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    18841 aacgcattgc aattcaagcc ggaaaaaatc gtcaagtgat cgatacgaca aaacgaaatc
    18901 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    18961 ttgaaattaa gattacggcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    19021 acaacaaaaa ccagcaataa cgcatcgcgt cgctgtggat attttattga gagaagaatt
    19081 tggagcggga aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca
    19141 actgagctat tcccgcttgg gtggtctgt
//
LOCUS       DAZGJT010000091        19064 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZGJT010000091 DAZGJT010000000
VERSION     DAZGJT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19064
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.091"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..183)
                     /locus_tag="ACHKFK_003556"
                     /pseudo
     CDS             complement(1..183)
                     /locus_tag="ACHKFK_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(483..4340)
                     /gene="sigA"
                     /locus_tag="ACHKFK_003557"
     CDS             complement(483..4340)
                     /gene="sigA"
                     /locus_tag="ACHKFK_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HFO9053815.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYALFNNDRVNNLKNNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVSGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKTAQGNNLKTGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQKHNNNTNLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGS
                     NNCLIPLLCQKDYSAAIRDQESTVNKRYNTEYKSNNQVASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQVHSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQDKEDNLDKTTHIPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNMSPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLIGKYVHHDNEYSATFAGLGTKDYSSHSLYAGAEAGYRYHV
                     TEDAWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPVIGRAGVDVGKSFSGKDW
                     KVTARAGVGYQFDLLNNGETVLRDASGEKRIKGEKDGRMLMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAVNASFRYSF"
     gene            complement(4702..5904)
                     /locus_tag="ACHKFK_003558"
     CDS             complement(4702..5904)
                     /locus_tag="ACHKFK_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO9053816.1"
                     /translation="MMMKKHITRTLVASAVLFSFNSAAATSYSEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPDDDFSLLLPSVGAQLSDPDNITDNADRISDDWKAFDRAID
                     SNHGVPEAAARLKERLRDFRHTHAAAQLGVSAVAALPGDRLSAALMVKSHGTVSVDGK
                     VSDADLTYLEEVANSTGQEVDKSRLTSQAFARAALITDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVRNYDSSAFKGDRYHNTKNGINADIGASTDLDDNWTLGLVAQN
                     LIPRSIETKEVNGITETFRIRPQVTAGVSWHNAMFTTAFDVDLTPASGFTSDSNRQFA
                     AIGAEFNAWKWAQLRAGYRQNLAGNDGSAFTAGVGISPFDVVHLDVAGLIGTDNTYGA
                     VAQFQFTF"
     gene            complement(5994..6815)
                     /locus_tag="ACHKFK_003559"
     CDS             complement(5994..6815)
                     /locus_tag="ACHKFK_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO9053817.1"
                     /translation="MKPNSTGIAARMILSLDRARICEALLNNRQLQPTPLQVRYPQGV
                     REALSIMSEQLSLSVSDLTRILVEEALSEMFLPADNSVRRLHDRMEYLMQVHELSPVT
                     MATLLAPWNIRPAVFREPDRLTDYLSGEILASLADWFYLSPEWLNGRVHYPLCHPGDW
                     PETQDAFCRLISEDDNIDIILWHGFPFAGEHAQEYCGVLLRQKKKINNAIIYPVLSLY
                     PTGMNKKKEGWFQIARKMSPDIPVRAVTLSPAQAEYLVTGKILPAELFRIPLSPW"
     gene            complement(7376..8500)
                     /locus_tag="ACHKFK_003560"
     CDS             complement(7376..8500)
                     /locus_tag="ACHKFK_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9053818.1"
                     /translation="MFRKIKIRKMTLNRALDKYLKTVSIHKKGHLQEFYRVNVIKRHP
                     MAERYMDEITTVDIATYRDQRLAQINPRTGRQITGNTVRLELALLSSLFNIASVEWGT
                     CRMNPVELVRKPKISSGRDRRLTSGEERRLSRYFRDKNQQLYVIFHLALETAMRQGEI
                     LTLRWEHLDLQHGVAHLPETKNGLPRDVPLSRKARNYLQILPQQINGNVFSYTSSGFK
                     SAWRTALLDLKIENLHFHDLRHEAISRFFELGTLNVMEVAAISGHRSLNMLKRYTHLR
                     AYQLVSKLDAKRKQTCKIAPYFVPYPATVGNRNGLFIVTLHDFDLETRAETRELAISH
                     ASVLLLRTLAQAAQRGERVPTPGELPANIDARVMICPLTS"
     gene            complement(8786..10004)
                     /gene="pilV"
                     /locus_tag="ACHKFK_003561"
                     /pseudo
     CDS             complement(8786..10004)
                     /gene="pilV"
                     /locus_tag="ACHKFK_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237204.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
     gene            complement(10001..10651)
                     /locus_tag="ACHKFK_003562"
     CDS             complement(10001..10651)
                     /locus_tag="ACHKFK_003562"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO9053819.1"
                     /translation="MSELLPICIILIVLAAILYLAYSAATVYLSQVHNTRPHNAAVCI
                     CCLLAVLSICLNGAYGVDATGLLFLSILTGLLTVMTLTDIAVCRLPRIFTLSLIILGA
                     AFRYSQDSLAHALLNASLWFGMIYLLRRIFLAVKGTEALGLGDVFLIAGIAMWTQPQH
                     TPLIITAAASGAFLFILIFCRRRRQQALPFAPFLCASLYVLTLFPDSVIHTSEIFT"
     gene            complement(10664..11140)
                     /locus_tag="ACHKFK_003563"
     CDS             complement(10664..11140)
                     /locus_tag="ACHKFK_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO9053820.1"
                     /translation="MKWLYFSVLILFSHTSIASVMNNNHLDSCFIQAGKRYLIAPELL
                     KTIAQQESGLVATAINHNKNKSGKIISTDYGIMQINSSHIPELKKLGVIKDKDELLKN
                     PCLNIHTGAWILARHFQRCGVNWECLGSYNAGFSKNNTARRMIYARIIHNRYFRKD"
     gene            complement(11199..11732)
                     /locus_tag="ACHKFK_003564"
     CDS             complement(11199..11732)
                     /locus_tag="ACHKFK_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO9053821.1"
                     /translation="MNFRARLFYYFSLKKEEEDKGLTLMEIAGALIIIGIVVGLAISQ
                     MSSTMSSSDNQRELKNLQTIATKMKQYKFQGQYTSSDYIKTLTQSGSLPADMIAGGNK
                     AKNAWGGDVTIKATTDKYGYTITSNNVPKENCVELINSLRSSSMFTKIMNTTPATVDP
                     VTVCSKDKNNITLETNS"
     gene            complement(11784..12884)
                     /locus_tag="ACHKFK_003565"
     CDS             complement(11784..12884)
                     /locus_tag="ACHKFK_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO9053822.1"
                     /translation="MKYLEKLHSTDIELTALTVWIARKTFALNRVEFYEALGGMIRDG
                     TPSLRALEFLCEIETDFGEKKGTSGMYFLAKECIASIKASGMLSGALFTWVPPEEATL
                     IRNGEERGNIADALFQVARTVKAQREMTSSLIAVCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFATEDKWSFQMKVLAGMSEFIIGYGLWLCGAIIALTVLIRFSFSRYTGPGRRML
                     DRVPPWSLYQTLHGVNYLFSISSMLQINIPLSQALLRMEKGAEKNKWLKERIQAIRKH
                     VLSGQNLATAMRNSGYDFPSRVCINKLLLNSEREDNVAAMAVYADQWLEEAKKRVKRS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            complement(12886..14406)
                     /locus_tag="ACHKFK_003566"
     CDS             complement(12886..14406)
                     /locus_tag="ACHKFK_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO9053823.1"
                     /translation="MPETIVLPPEDIRDFVAIQHDADGKIRVKVVEAKKHITSVQRYL
                     TLLRSRYPGKISKFDFTTLDVIATLNSNNTERAPGVLSDGGINTVQRKVLDYIRKAAQ
                     LDASDLHITPGRDNTDFTYVEARVHGELEVLDILRKEEGLELLGATYSGMTDVIKGTQ
                     FDPGVPQDARLSEQYLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSLQIPTFSMLGYH
                     PDQELTLRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQYGFNNTGGILLPRRRL
                     FTIESPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKA
                     AMTGHLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRRP
                     WHEVATDRTDDERRLVESVCNPDQVYLRNHEGCPHCWRGVTGRTVIAEVISPDARFFQ
                     LYREKGRIEARTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            complement(14416..14877)
                     /gene="pilP"
                     /locus_tag="ACHKFK_003567"
     CDS             complement(14416..14877)
                     /gene="pilP"
                     /locus_tag="ACHKFK_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO9053824.1"
                     /translation="MHDKTKALLIAALLLPAMAVAGNENDASQNKQLQIEQIQEETAL
                     YEAMLAREKIRHELRTLEQGISPVSGSVTGHQPENAAGTPSGSQALSSPRLKEIFGIQ
                     PQLSARVILPGGTETDVRAGQALPGTLFMVEQITPDSVILEYNGNRQTLRP"
     gene            complement(14867..16162)
                     /gene="pilO2"
                     /locus_tag="ACHKFK_003568"
     CDS             complement(14867..16162)
                     /gene="pilO2"
                     /locus_tag="ACHKFK_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO9053825.1"
                     /translation="MQKKIRPVSLISGKQAFLAGLDWKNIPPGYRNARRFARSQGAEL
                     YLSCHNHDGENGDEMMIALLSRQDADIPRNIRQCLSLAMLIRPLLKSGGYAIFIIGND
                     ENDDAELTCAFVSVINGILVNDVTGTPTEITEARNTFLMLNAEPENGWVRYEPDGFSP
                     DSRCIPLPLSNLISTRKHPAEARLLPVSRGPQLMTALLIITLLGASWYGWQRYEIWQA
                     EKAEREESARKSAEARITPPWTGQPETGEFIRQCSTTWNQTQLSAAGWRYTLAECSSD
                     GNSGNLRSSYTNTAGTTVTAFIRRITELTDTRPFIVMPEGNSGGVALPVTFRLPDNPV
                     PVDSLPETRDLQERLTTLAQSVQLQIDWQEVNNSFTDENGNIIQPPWKEYDLQIQTLL
                     PANQLAERFSEPSVRFLSVTRQLENGRFRYQFTGKYYAR"
     gene            complement(16186..17787)
                     /locus_tag="ACHKFK_003569"
     CDS             complement(16186..17787)
                     /locus_tag="ACHKFK_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO9053826.1"
                     /translation="MKKFFCAVTALSVVLSGCSSFERISATEKKASDEITRAERIAGE
                     LRKQRAVVRETNQQWINTTPLPEKTVTRAIPDCPVVINTREAITLHQIAQRISETCHI
                     PVQIMSDVWTLLGTGENRTQRISGDIPPPDTSGMQPLSTVGLSSAAPSTASLPELNVQ
                     GLPALLDTVSSRFGITWRYVKGKIEFHWLDTRTFPVTYMDSQVAYNARVVSGTMSSTG
                     SSGGSSGSSLSGDASNTQTTAVDMKSTLYEDTKNAVNSMLTPGIGRMVLSTGFLTVTD
                     TPQVLDTVKNFMEERNREMRRQVVLNIEILSIKKTSKEQAGIDWNAVFSDGHLGIDLT
                     SAFTNAADEVVTSGVSIIDGKLTGSKAFLKALSSQGKVSVVTQNSAVTKNLTPVPMQV
                     ASQQGYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFIQPGSHRLQLLFSMSLSD
                     KPVFEVFESGGSRAQTPSVELKTINQTIDLKSDQTVILSGFQQFSRKSLRQGVGTPSF
                     FGLGGGVDSADDDTILVVLITPHVI"
     gene            complement(17996..18406)
                     /gene="pilM"
                     /locus_tag="ACHKFK_003570"
     CDS             complement(17996..18406)
                     /gene="pilM"
                     /locus_tag="ACHKFK_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO9053827.1"
                     /translation="MALFIIGNWFHAGNTERAIKNTDEQQRQQQVYEILRLTNAINDW
                     KYHHPGDIREGKMLTVNETGFAPVYGTKHIISGQRTFVWLPFQPGLISAPNEVSRNSA
                     LIAQVRDRKLLDNQGNNIGVPPSVIPENAIVLIN"
     gene            complement(18431..>19064)
                     /locus_tag="ACHKFK_003571"
     CDS             complement(18431..>19064)
                     /locus_tag="ACHKFK_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001023520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO9053828.1"
                     /translation="FSFSYTATASTQISSSHSHAEKRAIGTLKFIRADGQRPFGQAMK
                     QLLPTGWRTEIARDVRLPARISWSGNDQWNHVIEKTLPRYSLKATFDTSGKRVLIQQA
                     EKTVKSGLFSAPVTGVKKTETRHIEQGMTLRTGLEKWAAAEPCATGKWKVAWKTPVNY
                     SIDARLVFSGNFMDALNSTFALYQQAEKPLYASVNKNQCVLIITDIPGDS"
BASE COUNT         4474 a         4446 c         4775 g         5369 t
ORIGIN      
        1 ggtcatgaaa gttatcttta tggatttact gatttgttcg aagagtttct gtgcgtgttt
       61 tattttatca tgctcttcat gtctgagttc gcgtttgcca tcaacatttt tggtttcaat
      121 aataaggttc agataatcac cctttgacgt gtttacaaca taagcgaagt cagttatggg
      181 gcttgccggc gttctccggg gggaaagctc cgtgctactg tcacctggaa agccgttgcc
      241 gcaagcttgc tcctcgtaag tgttaacgga gcgccgtatt gacgcttatt cactggtgag
      301 tactacgttc catggggggg cgtatgcgcc gccgatgacg cgcccgaaaa acgcgtcaat
      361 atgagtccgc cggaacgata cagagtttct gcaagtaaaa cacggtgttc tccggagcac
      421 gctattttat atttgtttta ttgaatataa gagggggcca taaagcggcc cccttgtttt
      481 tatcagaaag agtaacggaa gctggcgttc accgcgttat ccacgttgta cttaccaaat
      541 gccgatttct caaactccag accaaagcgg agattatcgc gaatctcagc attcagacca
      601 acattcatga gcatgcgtcc gtctttctcg cctttgattc gtttctcacc agaggcgtcc
      661 cgcagaacgg tttcaccgtt attcagcagg tcaaactgat aaccgacacc ggcacgggca
      721 gtcactttcc agtccttacc ggagaacgat ttacccacat ccacaccggc acgaccaatc
      781 actggattga agtccttgtc tttcatggtc acgctcattc cgcgatcctg ccagtcgaac
      841 cgtttacctg atacggcccc ataaaccagt tccgcctgcg gctcaatcca ggcgtcttct
      901 gttacatgat agcggtagcc tgcttcagca cctgcataca aggaatgaga actgtagtct
      961 tttgttccga gtccggcaaa ggtcgccgag tactcattat catggtgaac atacttacca
     1021 atcaggtcga tataggcacc agagtcaaat atagcggaag catacagacc tgccccgacg
     1081 gacttcgtct tgccactgaa tgcattactg ctggcatgac tgtcggtata cgtcatagtc
     1141 agaccagtga aaaggtcaag tccatccagc tcatgttttt tatccacacc aatctgcaca
     1201 tgggtgtagt tgtcactgta tccaccagaa gctgaacctg ctccgctcat gatgcgggcc
     1261 catgcaccgg cctcgccatt ggtgtcacgc agatccccca ttcgtttatt caggttgttg
     1321 acttctgtca ggaaggcttt gtaattaccg gacatatacg attttgctgc accggaagat
     1381 gctgtatttt cagccacctt atatccggtc agggtccacg tggtatgttc ctgatcctct
     1441 ttcgtgctga tcacaggaga catgttgctg aaacctatgg cctgagtact ggctttaaat
     1501 atctctttat tgcttccttt tggggctgtt atcaggtcaa cgtttaatcc tgagttatta
     1561 tttcccactt tctgtatatc gaccgacaga ctgttattgc ttccggttaa ctgattttta
     1621 acaatgagtt tatccgattg atttacagac tggctcagat cagcccgcat aatcacagca
     1681 gtattagtgg catccagtgt gtttactgtc acagtatgga aacccgaatt ctctatgtta
     1741 ctaaacaaca aaagactgtc gcgggttttt aatgattgca gggctgactg tccgtttact
     1801 ctccataagg tgttatacat gctggctgag gcagcaggag catcaatacc tccggtataa
     1861 gaagtatcaa agccacccag caaacctgta gccagcggta tgtgtgtggt tttgtccagg
     1921 ttatcttctt tatcctggtc aacaccaaac agaatagatg cttttttgtc tgattttata
     1981 ttaccattga cccaggcata gttttgagcc tttaacgtgg caccatcttc tgtcatatta
     2041 tatccatctg acatatagat tgccgggctg tatttattat tttctgtagg ggtgcctgtt
     2101 aatgtcaggg ttccggccag ttcaatggct ttatctacat acatttttcc ctgggcggta
     2161 acagtagctc ctttgttgat aatcaggtct gtattaatta aggataatgc accagtcata
     2221 gtaacagttg atttatcacc aacagttatt ttgctgtttt gagcactcag actgccgttg
     2281 aaactacttt cgcctgctga atccccggag tgaacctgtt gtctgaatcc aaaaccttcg
     2341 ccagtaatgt ttcttcctga gaacatgtca atgtatgcag ttttatttcc cagaacaatt
     2401 tgagagtttt tagcctctat gtgtcctttt acattagcat cccggcctat actcagggtt
     2461 gcgttttcta aatttaattt ccggaaatta aatttacgac tttcccagtc gggctgggaa
     2521 aaagaggcta cctgattgtt ggacttatat tccgtattgt aacgtttatt tacagtgctt
     2581 tcctggtccc ttatggcagc agaatagtct ttttgacata aaagaggaat tagacagttg
     2641 ttactgcctt ctttaaatat ggcatgttct gtcgcatgtc cttgtaatgt taactgtgca
     2701 ttccggacag agatatcgtt tttgatatcg actgagccat caaagatcag attggtatta
     2761 ttgttatgtt tctgggtagt attgataata tgatttatgc ttatattacc tgatacattc
     2821 ccatggtaca tatatgcatc agtattagta agtgataatg ttgattgctt cacgtttgag
     2881 ttcgttacgg ttgttcccgc atctgttgct gcgattttct gaaatgactg gtcatagccg
     2941 tttagatcca gtgttccgcc atttctggta aaatagattt cgccattacc gctttcgctt
     3001 aaagcgtctt tggcatttat ttttaccgta cctttacctc cggccatgta aattttattg
     3061 aaggcttttt cagcactaag gatgacagtc ccattacctg ttttaaggtt atttccctgt
     3121 gctgttttta catccagagt accactacct attttatgca gattatctcc ggcaacgccc
     3181 ttaacattcc attcaacggt ggtattatta tcaatatcta tcccggcacc tttataagac
     3241 ttatcttttc cggacacttt atatgtctgg ttcttatcga atataagccc gccaatgcca
     3301 agatcggtat cctgcttgag cgttaattcc cctcctcctg agacagacag gtccttatct
     3361 tttataatcg atgataactc ttgtttttta ccatccagct gcatagtgtt tgtgaaatta
     3421 ttttttaaat tgtttacccg gtcattatta aataacgcgt agtttgtcca ggctatattg
     3481 gtactactga taaaaccaac tgtactggta acaccatgga aaacccattt ttgttgttct
     3541 ttatcatata gataagaacc agaaccacta tcgccaaaaa ttggtgagtt tttaaaaggg
     3601 gttctgttgg tataaatgta actcttgttt ttccagtcca gattaaaaag acttgcgctt
     3661 aacaagtcgg gattatagtt ttgtccaaac agatattgtt tgccgtttaa tatcgttgaa
     3721 gttgttccgg cacctgctct gaagccaatt atttgttcct taccgttgta gtttacgcca
     3781 tatcttttca tcgcatcttc gggagaaagt gagaagtcgg cactctccgt aacccctgtt
     3841 gtttccacga cgaatttatt cagacgatga accgaaaagt cgccagatga tactctgtta
     3901 cttgctttat atgctgtctg atcccatgac tgtgttgtta ttgcatgatg ctgtgtaccg
     3961 ttatgagtcg cagtaacact gtacgcacct ccaattgatg ttgttgcacc tttgttggaa
     4021 acagcagaaa agtcaggtac aggtagttct ggaaaatgaa atatctttcc atctttcatc
     4081 attaattgaa catttgttgc acccggtctg aactctcctc tgttttgagc aaggtcaaga
     4141 taatcgcggg tccatatatt atttatatcc agcattgctg caaatgctgt ttcgccataa
     4201 cttccatata ttgaggataa aataataccg aggataactt tctttcttga tgtaccgaca
     4261 ctaactttcc gggtcagttc agaaacagca actaatccac ctgtaatatg actatatttc
     4321 agtgaataaa ttttattcat acgtgaactc cgatatttct gagttgtggt tatgggataa
     4381 catttaatta caacgggtaa ctatcagtca cggcacgtaa ctgatagggg tataatgata
     4441 atcactgtca gttatatttg tatgacagat gtaacaaaat aaaaaaaaaa gataaacaca
     4501 agtgattgat tgatcagata ttgatcactt ggtgtttgtg ttttgaactc tgtgatcaaa
     4561 atttgatctt tgttgtttcc ggtcaatcct ttacaggttt tctgtattat ccgggggagt
     4621 taagtctgtc gatgtccgac agacttttga tgatggaaac aacatgcagc gataatgtgc
     4681 atgctgtttt gcaggtggaa ctcagaaggt gaactggaac tgtgcgaccg caccgtaagt
     4741 gttgtccgtg ccgatcaggc ctgcaacatc aaggtgaacc acatcaaacg gtgagatccc
     4801 caccccggcc gtgaatgcac tgccgtcgtt accggccaga ttctgacggt aaccggcgcg
     4861 cagctgtgcc catttccagg cattaaattc tgcgccaatg gcggcaaact gacggttgct
     4921 gtcggaggtg aaaccgctgg ccggggtcag atccacatca aatgcggtgg tgaacatcgc
     4981 gttgtgccag gagacaccgg ccgtcacctg cgggcggatc ctgaaggttt ccgtgatacc
     5041 gttcacttct tttgtctcaa tactacgggg gatcaggttc tgtgcgacca gccccagcgt
     5101 ccagttgtca tccaggtccg tactggcacc gatgtcggcg ttgatgccat ttttcgtgtt
     5161 gtggtagcgg tcgcctttga aggcgctgct gtcataattt ctgaccagca cgttgtagtt
     5221 gaacaggtcg acgcgctgga atttgggggt gaagcccaga gaccatttct gcccggccgt
     5281 ttccagttcc gtagccagag caatacccac atcagtgatc agtgccgcgc gggcaaaggc
     5341 ctgtgatgtc aggcggcttt tgtccacctc ctgtccggtg ctgtttgcca cctcttccag
     5401 gtaagtgagg tcagcatcag agactttacc gtcgacactg acggtgccgt gtgatttaac
     5461 catcagtgcg gcagagaggc ggtcaccggg aagggccgcc acagcagaaa cgcccaactg
     5521 tgcggcggca tgtgtgtgac ggaagtcccg gaggcgttct ttcaggcgtg cggcagcctc
     5581 aggaacaccg tggttactgt cgatggcgcg gtcgaaggcc ttccagtcat cactgatccg
     5641 gtcggcgttg tcggtgatat tatccgggtc ggagagttgc gcaccgacgg acggaagcag
     5701 aaggctgaaa tcatcgtcag gaccggcttt tgtcagcagc gccgggtttg ccagcggtgc
     5761 cacgccatag tgagaggagg cgacgccggt tccgcccatt gcatcattac gggcttcaga
     5821 atatgaggtg gcggccgctg aattaaagga aaataaaacg gcagacgcga caagtgtacg
     5881 ggtaatatgc tttttcatca tcatttcagt ttcctgtatt aaggtctttc gggtatagca
     5941 aaggcgtggc acgcctgtgc cattaaaaac agtcagtgtt taataacgtg ttattaccag
     6001 ggcgataagg gaattcggaa taactcagcc ggtaatattt ttcctgtgac cagatattcc
     6061 gcctgtgccg gactgagcgt gacggccctg acggggatat ccggtgacat ttttctggct
     6121 atctgaaacc agccctcttt ctttttgttc attcctgtcg gatataaaga aagtaccggg
     6181 taaataatgg cattgttaat tttctttttc tggcgcagaa gaacgccaca atattcctga
     6241 gcgtgttcac cggcaaaagg aaaaccatgc cagagaataa tatcgatatt atcgtcttct
     6301 gaaataagac ggcagaacgc gtcctgtgtt tcgggccagt ctcccggatg acacagcgga
     6361 taatgcaccc tgccgttcag ccattcagga ctgagataaa accagtcagc cagtgaggca
     6421 aggatttccc cgcttaggta gtcagtgagt ctgtccggct cccggaagac ggcagggcgg
     6481 atattccagg gggccagtaa tgtggccatg gtgaccgggc tgagctcatg cacctgcatg
     6541 agatattcca tacggtcgtg aagacggcgg acgctgttat ccgcggggag aaacatctca
     6601 cttaatgcct cttcgaccag gatacgcgtc aggtcagaga cggagaggga aagctgttcg
     6661 ctcatgatgc tgagggcttc cctgacgccc tgtggataac gaacctgcag tggcgtgggc
     6721 tgcagctggc ggttgttgag cagggcttca caaatccggg ccctgtccag ggagagtatc
     6781 atgcgggcag caatgccagt gctgtttggt ttcattctgt tctccgtgcg gctcagcgcg
     6841 ttgtgttcat aagttgagtc taaagagagt gttttttaaa cggaaatgag ttatttcgat
     6901 cgttatgatc gagtgtttta aaagagtgtt aaccactctt tgtaggggcc cggggctggg
     6961 tgtggccaga tatgttgttg gtctgcattc tgcggcagaa gtggttattt gtattgcgtg
     7021 gggagtattg caggcgccct tgtttataag gtcataaggt tgtttccaga actgttgttt
     7081 attacatgaa gtcgtataat attaaaaaag tgaatccggc agtatataac actaacagaa
     7141 taatttgctt tggcagtgat agtttcttaa atctgtcact gtcatatgcc agccagaatg
     7201 cagtacagat ggccaggcct gataagaata tggatataag taatataatg gggagtggaa
     7261 tatgtccgga taccggaata ttatgccaca aatatatctg tatcaggacc tgagacagaa
     7321 tgactgtgat taagttaatc cggaatgtat agtttatttt cattctgata tgccttcagc
     7381 ttgttaacgg acaaatcatg acccgtgcat caatgtttgc gggtagctcg cctggcgttg
     7441 gaacgcgttc accccgctga gccgcctgtg ccagtgtgcg cagcaacaat acgctggcat
     7501 gagatattgc cagttcccgg gtttcagcac gggtttcaag gtcgaaatcg tgcagtgtca
     7561 caatgaacag tccgtttctg ttcccgactg ttgcgggata gggaacaaaa tagggggcga
     7621 ttttgcaggt ctgtttacgc tttgcatcga gcttgctgac cagctgatac gcccgcagat
     7681 gagtatatcg cttcagcatg ttgagagagc gatggccgga aatggccgca acctccatga
     7741 cgttgagtgt gccgagttca aaaaaacggc tgatggcttc atggcgaagg tcgtgaaaat
     7801 gcagattttc tattttcagg tccagaagtg cggtacgcca ggcgcttttg aaaccggatg
     7861 aggtgtaact gaacacattg ccgtttatct gttgtggcag aatttgcagg tagttgcgag
     7921 ctttccggga gagtgggaca tcgcgtggta aaccattttt cgtttcagga agatgcgcca
     7981 ctccgtgctg caaatcgagg tgttcccagc gtaatgtgag gatttccccc tgtcgcattg
     8041 ctgtttccag ggccagatgg aagataacgt agagctgctg atttttatct ctgaaatagc
     8101 gggagagtcg gcgttcttcg ccagaagtca ggcgacgatc gcgtccacta ctgattttgg
     8161 gttttcggac cagttcaaca ggattcatac ggcatgtccc ccattccaca ctggcgatat
     8221 tgaataatga tgataaaaga gcaagttcaa gacggacggt gtttccggtg atttgacgcc
     8281 ctgttcgtgg gttaatttga gccaaacgct gatcccggta agtggcgata tcaacggtgg
     8341 tgatttcatc catatagcgc tcggccatag ggtgacgttt aatcacattg acgcgataaa
     8401 actcctgcag atgacctttt ttgtggatgg atactgtctt caggtatttg tccagcgccc
     8461 ggttgagagt cattttgcga atttttattt ttcgaaacat atctgccttc cacgataaat
     8521 cccaggaagg taggaatact gaatcagaaa tgcaatgtct ctgtgccaaa tccggtttgt
     8581 ggcttggagg caataaagtg aactattctg cctgtaaatg gtatgaagcg acctccgcac
     8641 attttatcgg tggcagaagc ggaggttcta tttattacaa gccgattcag tgccctgccg
     8701 ggtacgtcat gacaggtaca agaatgtacg gcatcggaga tggtgttgat gaagagcacg
     8761 tcgatgctta ctgttgccct tttggttaga ttccggcacc aggaaggttg aggcatgtca
     8821 ctgtaattgt gccaccggac tcataatttc cccacgcata attattcaca taccaaacct
     8881 tgtttttgtc ggcgtatatc tgctgaaaac taccaaaagg cgagccatta aggtatgtcc
     8941 acgagcagta cgcatgtacg ccaatgctca gattacgagt atttttcccg atattgtatg
     9001 tactggtagt gaagttcatt tttgtcgttg tccagatacc ggatttggca ctgaagtatg
     9061 gccccgctgt tgtcgcggcc tacaagcccg tctggtgagc aatgagtgcc tgcgttggcg
     9121 attccgttta gctgtaaata ctcgccggca gtaagacgac catcagagcg gaccgttcct
     9181 ccagtgactg tctggccacg gacagttcca cctttgatct ctccttcggt tgtgatccct
     9241 ttgttgttaa cagcacgtat ccactggctg tccgtcatgg tgaagccgcc accatttgga
     9301 tgtaaccaac gatttgatgt gacatcgcct gaaaatctgg cgtttttacc actggcttca
     9361 ttgatattgg tgatgctgtt attattcata tcaatgccag tatgcattct gtttaaatcg
     9421 ggtcttcccg gaacgctgta gcggtaaagc cgatcgcttt cctccagatc ttttccagcc
     9481 attcgcatgg cgatatggcc cttgttgcag gtaatctgat aattacttcc cagatcggtc
     9541 cagccgccaa aggcacctgt aacggaatta ttctggagca taaaaccacc gagtccatta
     9601 atctggcccg ctatgcgacg taacccatct tctttaatat cctgtcctcc ggttgtgcag
     9661 gtaagtgctt ccagacgacc ggataaatta ttttttctga taccggtaat atagctgtgt
     9721 ccaaaggggg agcgggaaaa tccgttgttc agatatccct gctgaattaa ttcatttacc
     9781 gtgatggttt tactggaaag tgagctggtt ttatcagcta cataattttt tattgcatta
     9841 ttatattcat cagcatgtaa agccgcactt tgataaagtt gttcattcag ccagtcttca
     9901 atttctgaat aaatcattgg tgtggcgata ccaattacta tcaggccagc aagaatttca
     9961 agcagaataa aacctttatc gtcatcgagt ctgaattttt tcacgtgaat atctccgatg
    10021 tgtggataac ggaatcaggg aagagggtca gcacatacag ggacgcgcag agaaacggag
    10081 caaatggcag tgcctgctgg cgtctgcgac ggcagaaaat gaggataaac agaaatgctc
    10141 cggaggctgc ggcggtgatt atcagtggag tgtgttgcgg ttgtgtccac attgcgatcc
    10201 ccgcaatcag gaacacatca ccaaggccga gcgcttccgt ccctttcact gccagaaaaa
    10261 tgcgtcgtaa caggtatatc atgccgaacc acagtgacgc gttgagcagg gcatgagcca
    10321 gtgagtcctg gctgtatctg aacgctgcgc ccagaataat aagagaaagc gtgaatatcc
    10381 ggggaagccg acagacagca atatccgtca gcgtcattac agttaataat cccgtcagaa
    10441 tggaaaggaa taacagtcct gtcgcgtcaa caccatatgc accgttgagg caaatgctca
    10501 gtacagccag aagacaacag atgcacaccg cagcgttgtg tgggcgggta ttgtgtacct
    10561 gcgacaaata taccgtggcg gcgctgtatg caagatacag aattgccgca aggacaatca
    10621 ggataatgca tatcgggagt aattcagaca cagtcacctc agcttaatct tttctgaaat
    10681 accggttgtg gattattcgg gcatatatca tgcgccgggc tgtgttattt ttgctgaatc
    10741 ctgcgttgta actgccaagg cattcccagt taacgccgca gcgttgaaaa tggcgggcca
    10801 gtatccaggc gccggtatga atattcaggc agggattttt aagcagttca tccttgtctt
    10861 taatgacgcc cagttttttc agttcaggaa tatgcgatga attaatctgc attattccgt
    10921 aatccgtact gatgattttt ccggatttgt ttttattgtg atttatcgca gtggcgacca
    10981 ggccgctttc ctgctgtgca atggttttca gaagctcagg ggcaatcagg taacgttttc
    11041 cggcctgaat aaaacaggaa tcgagatgat tattattcat cacagatgcg attgatgtat
    11101 gactgaataa aatcaggaca gagaaatata accatttcat tatgataatc ccggcagtga
    11161 cagcatcctc ttgtatttaa ggatgctgtc cggaaacatt agctgtttgt ttcaagggtg
    11221 atgttatttt tgtccttact acatactgtt acaggatcca cagtagccgg ggtggtattc
    11281 atgattttgg taaacatgga tgaactgcgc aggctgttaa tgagttccac gcaattttct
    11341 ttcggtacat tattgctggt aatcgtataa ccatacttat ctgttgttgc cttaatggtg
    11401 acatcaccgc cccaggcatt tttggccttg ttaccaccgg cgatcatatc tgccggcagg
    11461 gaaccactct gtgtcagcgt tttgatataa tcactgctgg tatattgtcc ctggaacttg
    11521 tactgcttca ttttggtggc aatggtctgc aggtttttca gttcgcgctg gttatcactg
    11581 gatgacatgg tactggacat ctgacttatt gcgagaccaa ccacaatacc gataataatc
    11641 agtgcgccgg caatttccat taaggttaaa cctttatcct cctcttcttt ttttaaagaa
    11701 aaataataaa aaagacgtgc tctgaagttc atatatcact cctggtttat gggatgtttc
    11761 agaaatattc tgtatggtgg gggttattgt ttcagcatgt cgctgatact gtagatggca
    11821 gatatcatgt taagaataaa aaagaagaca aaacctcctg atataccggt aataattaat
    11881 ccgctgcgtt ttacccgttt ttttgcctct tccagccact ggtcggcata gaccgccatg
    11941 gctgccacat tatcctccct ttcggaattc aggagcagtt tattgatgca tactctggac
    12001 ggaaaatcgt aaccggaatt tcgcattgcc gtggccagat tctgaccgga taacacatgt
    12061 tttcgtattg cctgaattct ctcctttaac catttattct tttctgcccc tttttccatt
    12121 ctgagcagtg cctgcgaaag cggaatatta atctgcagca tggatgaaat actgaacaga
    12181 taattcactc cgtgtaaggt ctgatacagt gaccagggtg gtaccctgtc cagcatcctc
    12241 cggccggggc cggtgtacct gctgaaggaa aaacggatca gaaccgtcag ggcgataatg
    12301 gccccgcaga gccagagtcc gtacccgatg ataaattccg acatgccggc cagcactttc
    12361 atctggaatg accatttatc ttccgtggca aacgcactga cgatgggaat gatacgggta
    12421 tgggcgttgt acatgttcac cacacagagt gaaagcagca gaaggggata gaggcagacg
    12481 gcaatgaggc tggatgtcat ttcccgctgg gctttaacgg ttctggccac ctggaaaagg
    12541 gcgtcggcaa tattcccgcg ctcttccccg tttctgatta acgtggcttc ctccggtggc
    12601 acccaggtga acagggcacc ggataacatg ccgctggctt tgatggacgc gatgcattct
    12661 tttgccagaa aatacatgcc ggatgtgcct ttcttttcac caaaatcggt ttcgatctcg
    12721 cagagaaatt cgagggcacg cagggacggc gttccgtcac gtatcatgcc gcccagggct
    12781 tcgtagaact ccacccggtt cagggcaaag gtttttctgg ctatccagac ggtcagtgct
    12841 gtcagctcga tgtcggtact gtggagtttt tccagatatt tcatatcagt gcagcagcgt
    12901 atagctgtcc tcatcgacgg gagagatgta atgggccgcc agcggatcaa cctgtccgct
    12961 gttgattttt cccagcagat gctgattacg ggtcattccg cccagttccc ggtgccagta
    13021 ggttctggcc tcgatacgcc ccttttcccg gtacagctgg aaaaaacgcg catccgggga
    13081 gataacttct gcgatcaccg ttcgccccgt cacgccacgc cagcagtgcg ggcagccttc
    13141 atggtttctc agatatacct ggtccgggtt acagacgctt tccaccaggc gccgttcatc
    13201 atccgtgcgg tcggtggcca cctcatgcca ggggcgacgg cagtgcggac atatcagctg
    13261 aaccagacgc tggctcagca ggccaatgaa cagctgcgga tcggcaatca tccttgcctg
    13321 taccccctcc atctcaagac gttcgagaat gttaatcggg tcgttcgcat ggatggtggt
    13381 cagcatcaga tgcccggtca tggcggcttt tattgccgta atggcggaat cgtggtcacg
    13441 gatttcgccg ttcaggatcc cgtcagggtc aagacgcagt gctgatttaa tggagtccac
    13501 ccagccctgt gcggaatcca tgacggcagt ctgaatggca ccgggtatac ggccctctgg
    13561 cggggattca atggtgaaca gcctgcgacg gggcagaaga ataccgccgg tattgttgaa
    13621 gccgtactgt tcaaggtatg ccgcagaggc ggttctcagc gtggtggatt ttcctgaacc
    13681 tgttggtcct gagagcgtga taataccttc agggcgctgc agaatacggc gcagggtgag
    13741 ctcctggtca gggtgatagc ccagcatgct gaaggtcggg atttgcagac tgtcatcctt
    13801 gatcagacgg agcacagcat agaggccgcc cacgcaggga tagtgggagt agcgtgcacc
    13861 aaacagaccg gccagtttca ggtactgctc tgaaagacgc gcatcctgag gcaccccggg
    13921 atcaaactgt gtgcccttga tgacgtcggt catgccggaa taggtggccc ccagtaattc
    13981 gagaccttct tctttccgga ggatatcgag cacttccagt tcgccgtgaa cccgggcttc
    14041 cacataggtg aagtccgtgt tgtcgcgtcc tggtgtgata tgcagatctg aggcatccag
    14101 ttgagcggct tttctgatgt aatcaagaac ttttctttgt actgtgttga tccctccgtc
    14161 ggataaaacg cctggtgcac gttccgtatt attgctgttc agggttgcga taacgtcaag
    14221 agtggtaaaa tcgaatttgc tgatttttcc cggatagcgg gagcgtaaga gcgtcaggta
    14281 acgttgtaca gaggtgatgt gcttcttcgc ctcgacgact ttcaccctga tttttccgtc
    14341 agcgtcatgc tgaattgcga cgaaatcacg aatatcttca ggaggcagta cgatagtctc
    14401 tggcatgatg gcacctcacg ggcgtagcgt ctgcctgttg ccgttgtatt ccagaatcac
    14461 actgtccggt gtgatttgtt ccaccatgaa cagtgtaccg ggtaatgcct gtccggccct
    14521 gacatcagtt tcagttcctc cggggagtat gacgcgtgct gaaagctgtg gctgaatacc
    14581 aaagatttct ttcagacggg gggatgataa tgcctgtgaa ccggaggggg tgccagctgc
    14641 attttccggc tgatgtcccg tgacagaacc ggaaaccggg gagataccct gttccagtgt
    14701 ccgcaattca tgacgaattt tttctcttgc cagcatcgct tcatacaggg cggtttcttc
    14761 ctgaatctgc tcaatctgca gttgtttgtt ctgtgacgcg tcattttcgt tgccggccac
    14821 tgccattgcc ggtaacagca gggctgcaat cagcagcgct tttgttttat cgtgcataat
    14881 atttacctgt gaactgataa cggaaacgac cattttccag ttgtctagtg acggagagaa
    14941 aacggacaga tggctcgctg aagcgctctg ccagttggtt agccgggagt agcgtctgta
    15001 tctgcaggtc atactctttc cacggtggct ggatgatgtt tccgttttca tcagtgaagc
    15061 tgttattgac ttcctgccag tcaatctgca gttgaactga ctgtgcgagc gtggtcagtc
    15121 gttcctgcag gtctcgggtt tccggaaggg aatcaaccgg gacaggatta tccgggagcc
    15181 ggaatgtgac cggcagtgca acgccgccag agttcccttc aggcataaca ataaacggcc
    15241 gggtgtctgt gagttcagtg atgcgccgga tgaaggctgt cactgtcgtt cctgctgtat
    15301 tggtgtatga agaccgcaga ttaccgctgt taccgtcaga agaacattca gccagggtat
    15361 aacgccatcc ggcagcagac agttgcgtct ggttccatgt tgtgctgcat tgccggataa
    15421 attctccggt ctcaggctgc cccgtccatg gtggcgttat acgtgcttca gctgatttcc
    15481 gggcgctttc ttcccgttcc gctttttccg cctgccagat ttcataacgt tgccagccgt
    15541 accacgatgc gccaagcagc gtgatgatga gtaaggctgt cattaactgt gggcctctgg
    15601 acacggggag cagacgcgct tctgccggat gctttctggt gctgatcagg tttgacagcg
    15661 gtagcgggat acaccgactg tctggtgaaa aaccgtcagg ttcgtagcgt acccagccgt
    15721 tttccggttc cgcattgagc atcaggaacg tgttgcgggc ttccgtgatt tcagtcgggg
    15781 taccggtaac gtcgttaacc agaatgccat taatcaccga aacaaacgca caggtgagtt
    15841 cagcgtcgtc gttctcgtcg ttcccgatga tgaaaatggc atacccacct gatttgagta
    15901 gcgggcgtat taacattgcc agtgacaggc actggcgaat gttacggggg atatctgcat
    15961 cctgacgact gagcagggca atcatcattt catcaccgtt ttcaccgtcg tggttgtggc
    16021 aggaaagata cagttctgcg ccctgtgacc gggcaaaccg ccgggcattc cggtaaccgg
    16081 gaggtatgtt cttccagtca aggcccgcaa ggaatgcctg tttacccgat atcagtgaaa
    16141 ccggacgaat ttttttctgc attgacccgt tcaagggaac ctccgtcaga ttacgtgcgg
    16201 tgtgatcagc acaaccagaa tggtgtcgtc atccgcactg tccacgccac caccgagtcc
    16261 gaagaatgac ggggtgccca cgccctgtct gagcgatttc cggctgaact gctggaatcc
    16321 gctcaggatc accgtctgat ctgatttcag gtcgatggtc tggttgatgg ttttcagctc
    16381 aacactcggt gtctgggccc gactgccacc gctttcaaac acttcaaaaa caggtttgtc
    16441 ggacaggctc atgctgaaca gtaactgcaa ccggtgtgag ccgggctgga taaacggtaa
    16501 cagtgtcatg ttgaagccgg tggtgattgt tgccgcattc agtgaggtgg aggagcccac
    16561 attggccgtc gtatccgtgg tgacactttc tatgtacccc tgctggctgg cgacctgcat
    16621 aggaaccggc gtcaggtttt tggtgacagc cgagttttgg gtgaccacac tgactttacc
    16681 ctgtgatgaa agcgctttca gaaaggcttt tgaaccggtg agcttaccgt caataatcga
    16741 taccccactg gtcacgactt catcggcggc attagtaaat gcactggtca ggtcgatacc
    16801 gagatgtccg tcactgaaaa cggcgttcca gtcgattccg gcctgctctt tgctggtttt
    16861 tttgatgctc agaatttcaa tattcagcac aacctgacgg cgcatttccc ggttacgttc
    16921 ttccataaaa ttcttaacag tatccagaac ctgtggggtg tcggttacgg tcagaaagcc
    16981 ggtggacaac accatccttc cgataccggg cgtcagcatg gaattcacgg cattttttgt
    17041 gtcttcatag agtgtggatt tcatatcaac ggcggttgtc tgcgtattgc tggcatcccc
    17101 actcagggaa ctgccggatg aaccaccgga actgcccgtg gatgacatgg tgccactgac
    17161 gactctggca ttgtaggcga cctggctgtc catgtaggtg accgggaatg tacgggtgtc
    17221 aagccagtgg aattcaattt ttcccttcac atagcgccag gtgatcccaa agcgtgaaga
    17281 tacggtgtca agcaaagcgg gcagcccctg aacattgagt tccggcagtg aggccgtgga
    17341 gggggcggct gatgacagcc ccacggtgct cagtggctgc atgccactgg tatcaggcgg
    17401 gggaatgtcg ccgctgatcc tctgtgtgcg attttctccg gtacccagca gtgtccagac
    17461 atcagacatg atttgaaccg gaatatgaca ggtttcagaa atccgctgtg caatctggtg
    17521 aagtgtgatg gcttcccggg tgtttatgac gaccgggcaa tccggtattg cccgggtaac
    17581 ggttttttcc ggaaggggcg tggtattgat ccactgctgg tttgtttcgc gcacaacggc
    17641 gcgttgtttg cgaagttcac cggctatacg ttcagcccgt gttatctcat cggatgcttt
    17701 tttttctgtg gcactgattc gttcaaaact gctgcagcct gataaaacaa cggataaggc
    17761 ggtgacagcg cagaaaaatt ttttcatatt ctgtatacct gcttgttgaa tatttattct
    17821 ctgtctgtgg agggacgaaa taacaattca gtgagacaga gcgacgggta tttcatgtgt
    17881 taatgaacac tgagaaagat tttccggact gaagtctgcg ccgtagcgat atatcatgtt
    17941 gctgagagaa gacatgatat gcagacctca gtgcagaatc cccttttggg gatgcttaat
    18001 tgatcaggac gatcgcattt tccggtataa cggaaggcgg aacaccaata ttatttccct
    18061 ggttatccag taactttctg tccctgacct gcgcaattaa tgctgagttt cggctcactt
    18121 cattcggggc tgatatcagc cctggctgaa aaggaagcca gacaaaagtt ctttgccctg
    18181 aaataatatg ttttgttcca tataccggag cgaatccggt ttcgttaacc gtaagcatct
    18241 ttccttcacg gatatcaccg ggatgatgat atttccagtc attaatggca tttgtcaggc
    18301 gtaatatttc atatacctgt tgttgtcttt gctgctcatc agtatttttt attgcccgct
    18361 ccgtattgcc ggcatgaaac cagttcccga tgataaacag tgccattatg aggggcatga
    18421 gccatcccat ttagctgtcc cccgggatgt cagtaattat cagcacgcac tggtttttat
    18481 tcaccgaggc atataagggt ttttcagcct gctgataaag tgcgaatgta ctgttcagtg
    18541 catccataaa attacctgaa aaaactaacc gggcatcgat actgtaattt accggcgttt
    18601 tccatgccac tttccatttc ccggtagcgc agggttcggc ggctgcccat ttttccagcc
    18661 ctgtacgcag ggtcattccc tgctcaatat gacgtgtttc tgtttttttg acaccggtta
    18721 cgggggcaga aaacaggcca gattttaccg ttttttccgc ctgctggatc agtacgcgtt
    18781 tccctgaggt atcaaatgtg gctttcaggc tgtagcgggg cagcgttttt tcgataacat
    18841 ggttccactg gtcatttccg gaccatgata ttctggcggg gagccggacg tcacgggcaa
    18901 tctcagtacg ccagcctgtg ggtaataatt gtttcatagc ctgaccaaac ggtcgttgtc
    18961 cgtcggcccg tataaatttc agcgtaccga ttgcacgttt ttcggcgtgg ctgtgcgatg
    19021 atgatatctg agtggaggct gtggcggtat atgaaaatga gaac
//
LOCUS       DAZGJT010000092        18936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZGJT010000092 DAZGJT010000000
VERSION     DAZGJT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.092"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..339
                     /locus_tag="ACHKFK_003572"
     CDS             <1..339
                     /locus_tag="ACHKFK_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO9053829.1"
                     /translation="ACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQV
                     QYSMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDEALAE
                     IGFVYGEQWQ"
     gene            336..494
                     /locus_tag="ACHKFK_003573"
     CDS             336..494
                     /locus_tag="ACHKFK_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001598316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFO9053830.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRSWVFPGLSVIRNPLKAQRLAEE
                     INNKRESV"
     gene            491..1243
                     /locus_tag="ACHKFK_003574"
                     /pseudo
     CDS             491..1243
                     /locus_tag="ACHKFK_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1251..1466
                     /locus_tag="ACHKFK_003575"
     CDS             1251..1466
                     /locus_tag="ACHKFK_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO9053831.1"
                     /translation="MTIEHNNALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1565..1786
                     /locus_tag="ACHKFK_003576"
     CDS             1565..1786
                     /locus_tag="ACHKFK_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO9053832.1"
                     /translation="MADLIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            1783..2568
                     /locus_tag="ACHKFK_003577"
     CDS             1783..2568
                     /locus_tag="ACHKFK_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9053833.1"
                     /translation="MSEINYQELREKAEKATKGSYIVGHTSVNQHGNLTGVFVCQKWK
                     GEPGGVIAECHVNCLIESDAQAYANAEFIADANPATVLALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEETKSKLNEQREYYEGVISDGSKRIAELEKIATDYALKFQKAQD
                     ALKYAVLLRKSGQEAREIKPAKGEVLVVVSGFTGCGKSAIAGEIEIAMKSIGVPVKWT
                     NGDAEKRMTGADWLTAIEMYKPTVRIVEVNVPRAAGIRIKGGA"
     gene            2568..2744
                     /locus_tag="ACHKFK_003578"
     CDS             2568..2744
                     /locus_tag="ACHKFK_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053834.1"
                     /translation="MRVAFIGLLPYPTRFWASALIAKPHVLMADNIIPAPKRRHTGIA
                     AARREAKKRRRAKR"
     gene            2920..3111
                     /locus_tag="ACHKFK_003579"
     CDS             2920..3111
                     /locus_tag="ACHKFK_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001420769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053835.1"
                     /translation="MKNRKAKMLISRVYRRCYPSQWLRVSNRRVVLYLYSGIAREGIK
                     DKRSAAQNRWKKHLRTKGA"
     gene            3113..3322
                     /locus_tag="ACHKFK_003580"
     CDS             3113..3322
                     /locus_tag="ACHKFK_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053836.1"
                     /translation="MSIAASYTMHLYCDCRQCTEGVYPVPDFGEYIGTSWSGCAKEDR
                     KDGWRISKDKTRAFAPGHKVLRINT"
     gene            3319..3444
                     /locus_tag="ACHKFK_003581"
                     /pseudo
     CDS             3319..3444
                     /locus_tag="ACHKFK_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            3442..3714
                     /locus_tag="ACHKFK_003582"
                     /pseudo
     CDS             3442..3714
                     /locus_tag="ACHKFK_003582"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
     gene            3898..4128
                     /locus_tag="ACHKFK_003583"
                     /pseudo
     CDS             3898..4128
                     /locus_tag="ACHKFK_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            4213..4455
                     /locus_tag="ACHKFK_003584"
     CDS             4213..4455
                     /locus_tag="ACHKFK_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9053837.1"
                     /translation="MFALIQRGQIYTDSAGYPIKIVRCINNTVLYRRMDGRTQSVKIN
                     DFNELFERIDHQEYRQILAETEQEAHLKKLRAMKRK"
     gene            4459..4593
                     /locus_tag="ACHKFK_003585"
     CDS             4459..4593
                     /locus_tag="ACHKFK_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9053838.1"
                     /translation="MNKAFERWVHQRYSNRYDLTRDVDGFYCREVVKRMFDVWCHCRG
                     "
     gene            4612..4866
                     /gene="xisR"
                     /locus_tag="ACHKFK_003586"
     CDS             4612..4866
                     /gene="xisR"
                     /locus_tag="ACHKFK_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9053839.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            4900..6186
                     /locus_tag="ACHKFK_003587"
     CDS             4900..6186
                     /locus_tag="ACHKFK_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9053840.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            6207..6908
                     /gene="mlrA"
                     /locus_tag="ACHKFK_003588"
     CDS             6207..6908
                     /gene="mlrA"
                     /locus_tag="ACHKFK_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO9053841.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYISICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGDNRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            6968..7075
                     /gene="yohO"
                     /locus_tag="ACHKFK_003589"
     CDS             6968..7075
                     /gene="yohO"
                     /locus_tag="ACHKFK_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO9053842.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(7056..7787)
                     /gene="yehW"
                     /locus_tag="ACHKFK_003590"
     CDS             complement(7056..7787)
                     /gene="yehW"
                     /locus_tag="ACHKFK_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO9053843.1"
                     /translation="MKMLRDQLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLKPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGANTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQAFSQHAK"
     gene            complement(7792..8718)
                     /gene="yehX"
                     /locus_tag="ACHKFK_003591"
     CDS             complement(7792..8718)
                     /gene="yehX"
                     /locus_tag="ACHKFK_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO9053844.1"
                     /translation="MIEFSHVSKLFGTQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLEPNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLQGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLAMLTHPANDFVRQFFGHSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGEPCGTLHFQDLLEEA"
     gene            complement(8711..9868)
                     /gene="yehY"
                     /locus_tag="ACHKFK_003592"
     CDS             complement(8711..9868)
                     /gene="yehY"
                     /locus_tag="ACHKFK_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO9053845.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTLWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIVPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(9875..10792)
                     /gene="osmF"
                     /locus_tag="ACHKFK_003593"
     CDS             complement(9875..10792)
                     /gene="osmF"
                     /locus_tag="ACHKFK_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO9053846.1"
                     /translation="MPLSKLWAGSLVLLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDTVWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLAELSRYLKEGGNF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLRPVFASLDEKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(10982..13279)
                     /gene="bglX"
                     /locus_tag="ACHKFK_003594"
     CDS             complement(10982..13279)
                     /gene="bglX"
                     /locus_tag="ACHKFK_003594"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO9053847.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKAEFELL"
     gene            13475..15190
                     /gene="dld"
                     /locus_tag="ACHKFK_003595"
     CDS             13475..15190
                     /gene="dld"
                     /locus_tag="ACHKFK_003595"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9053848.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHGHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(15229..16161)
                     /gene="pbpG"
                     /locus_tag="ACHKFK_003596"
     CDS             complement(15229..16161)
                     /gene="pbpG"
                     /locus_tag="ACHKFK_003596"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO9053849.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(16335..16922)
                     /gene="yohC"
                     /locus_tag="ACHKFK_003597"
     CDS             complement(16335..16922)
                     /gene="yohC"
                     /locus_tag="ACHKFK_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO9053850.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            17092..17670
                     /gene="yohD"
                     /locus_tag="ACHKFK_003598"
     CDS             17092..17670
                     /gene="yohD"
                     /locus_tag="ACHKFK_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9053851.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWSLIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(17790..18551)
                     /gene="yohF"
                     /locus_tag="ACHKFK_003599"
     CDS             complement(17790..18551)
                     /gene="yohF"
                     /locus_tag="ACHKFK_003599"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO9053852.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGNLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDGGDVKPDAEPSIPLRRFGTTHEIA
                     SLVVWLCSEGANYTTGQSLIVDGGFMLVNPQFNPE"
     gene            complement(18604..>18936)
                     /locus_tag="ACHKFK_003600"
     CDS             complement(18604..>18936)
                     /locus_tag="ACHKFK_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="may be involved in resistance to puromycin,
                     acriflavine and tetraphenylarsonium chloride; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO9053853.1"
                     /translation="NLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQ
                     AQIERDALRVVGLAQARFNAGLIAGSRVSEARIPALRERAHGLLLQGQWLDASIQLTG
                     ALGGGYKR"
BASE COUNT         4872 a         4913 c         4797 g         4354 t
ORIGIN      
        1 gcctgctctc ccgatggttt atgcagtgac ggcaatggcc ttgagctgaa atgcccgttt
       61 acctccaggg atttcatgaa gttccggctc ggtggtttcg aggccataaa gtcggcttac
      121 atggcccagg tgcagtacag catgtgggtg acgcgaaaag atgcctggta ctttgccaac
      181 tatgacccgc gtatgaagcg tgaaggcctg cattatgtcg tgattgagcg gaatgaaaag
      241 tacatggcga attttgacga gatggtgccg gagttcatcg aaaaaatgga cgaggcactg
      301 gctgaaattg gttttgtata tggggagcaa tggcaatgaa gcatcctcac gataatatcc
      361 gggtaggcgc gatcactttc gtctactccg ttacaaagcg aagctgggta tttcccggcc
      421 tttctgttat ccgaaatccg ctgaaagcac agcggctggc tgaggagata aataataaac
      481 gggagagtgt atgattcatt ttcacggtgg tccaataact cccgatacct gtgcgttgaa
      541 agcctggaaa ggcagacacg cattcatcag ttttgctaat ccagctcaga ttgatctggc
      601 ttccgaagtc acccaatcat ttgctcttga taatggtgca ttcacattct ggacaaaaaa
      661 caaggccata gactggaatg aatattacag atttgttgaa cgctggggta atcaccctcg
      721 tttctcattc gcggttatcc cggatgttat cggcggaacc agtgaagaga atgacgccct
      781 gattgcggca tggcctcacg gtaaatttat tggtgctccg gtgtggcaca tgaacgaacc
      841 agatgagcga tttattcgtc tgtgccatga gtttccccgc gtctgcatcg gctcgatggg
      901 ggaatacgat gcaaaacgac cgagagcgtg tcgggctaaa ctacgcgatc ttatacgtca
      961 tgttgtagat cagtacggct acccaatcac caagattcat gggttacgga tgttaaataa
     1021 agacattttt actcatgtac cgctttcgtc tgcagacagc acgaacgtcg caagaaatat
     1081 aggcatcgac aaatcctagg taggttcgcc atatgctccc gcaagtaaag aaacccgtac
     1141 gcaagttctt gtggaacgca ttgaatcatt caacagtgcc agttcactta attacaacgc
     1201 tgaacgggac gtctttaccc ctcaactggc attcgaagtg tgaggccaat atgacaatag
     1261 aacataataa cgcccttcgc agcattgccc gtcaggctaa ttctgaaatc aaaaaagcca
     1321 gacagcagtt tccggataaa aacgtcgatg acatttgccg tagcgtactg aagaagcacc
     1381 gcgaaacggt aacgctgatg ggattcacac cgactcactt aagtctggca atcggtatgt
     1441 taaacggcgt ctttaaggaa cggtgaacat gaaaagcaaa atcatcaggg agctacaggc
     1501 tcctttttta ttattcgcat tcaccctcaa gcgtattaac caacaattca gggattaatg
     1561 gaagatggca gacctcattg attcagcatc agaaattgaa gaattacagc gcaacacagc
     1621 aataaaaatg cgccgcctga accaccaggc tatatctgcc actcattgtt gtgagtgtgg
     1681 cgatcccata gatgagcgaa gacgcctggc cgttcagggt tgtcggactt gtgcaagttg
     1741 ccaggaggat ctggagctta tcagtaaaca gagaggttcg aagtgagcga aattaactat
     1801 caggaactgc gtgaaaaggc agagaaagca actaaaggaa gctacatcgt agggcataca
     1861 tctgttaacc agcacggcaa tttaacagga gtttttgttt gtcaaaaatg gaaaggagaa
     1921 cccggtggcg tgattgccga atgtcatgtt aactgcctga ttgaatcaga tgctcaggct
     1981 tatgcaaacg ctgaatttat agcagatgct aacccagcta ccgtactggc gctactggat
     2041 gaacgggaaa gaaaccagca atacatcaaa cgccgcgacc aggagaacga ggatattgcg
     2101 cttacggttg ggaagctgag agttgagctt gaggagacaa aatcaaaact caacgagcag
     2161 cgtgagtatt acgaaggtgt tatctcggat gggagtaagc gtattgctga actggagaaa
     2221 atcgccactg actatgcact gaaatttcag aaagcccagg acgcattaaa gtacgctgtt
     2281 ttgctgcgca aatcagggca ggaagcacgg gaaataaaac cagccaaagg cgaagttctt
     2341 gtcgttgtat ctggttttac tggttgcgga aaaagcgcca tcgccgggga aatagaaatc
     2401 gcgatgaagt ctattggtgt accggttaag tggactaatg gcgatgcgga aaagcgcatg
     2461 acaggagctg actggctgac agcgattgag atgtacaaac caacagtgcg catcgtggaa
     2521 gttaatgtgc cacgcgccgc aggcattcgc atcaaaggag gtgcgtaatg cgtgtcgcgt
     2581 ttatcggttt attaccatac ccgactcgtt tttgggcttc tgcgctaatt gcaaagccgc
     2641 atgtactgat ggctgacaac atcatcccgg caccaaagcg ccgccatacc ggtattgcgg
     2701 ctgcacgacg cgaagcaaag aaacgcagga gagcaaaacg atgaaaaacc gtaaggcaaa
     2761 aattctgtta gctcgcagaa acggtgttgg ggtctggcgc tggttgagga ttagtaacag
     2821 acgagtgagg ttgacggggt gttgcggggt gatggggcac agctgttgca aaaagcccag
     2881 cgcgtcacag aacaggcgga agaaagggta cgcagtaaga tgaaaaaccg taaagcaaag
     2941 atgcttattt cccgtgtata cagacgttgc tatcccagcc agtggttgag agttagcaat
     3001 cgccgtgtgg tgttgtactt atattctgga attgccagag agggaatcaa agataagcgc
     3061 agcgcggcgc aaaaccgctg gaaaaaacac ttgcgcacta aaggagcctg atatgtctat
     3121 tgccgcaagt tacaccatgc atctctattg tgactgccgc cagtgtacgg aaggtgtata
     3181 tccagtgcca gacttcggtg agtatatcgg tacgtcatgg tctggttgtg caaaagagga
     3241 ccgtaaagac gggtggcgaa taagcaaaga caaaacacgt gcttttgcgc cagggcataa
     3301 agttttaagg attaacacat gaccactatt accaaagaac gtattgaatt gttcattaaa
     3361 aatccgcttg aaaacgggct tactcgtggc gaacaaatgg aactggcacg aattgcactg
     3421 gcatcactgg aacgcgaact gattcgccac gagcatgcca aatggtctga ctccacattt
     3481 ggctgcgttg gccccattgg tccactgaaa cacctctcaa aagaggctct ggaagccgca
     3541 gccgaaccag acgatctcag cgagtgggct gatatgcatt tcctgttgtg ggatgcacag
     3601 cgccgttctg gcatcagcga tgctgaaatt accgctgcta tggaagataa attgaagatc
     3661 aacatggagc gccagtggcc tgaaccaaaa gatggtgagc ctcgcttgca cattaaagaa
     3721 cccggcaact ctccggtaat tccggatggt ttatccacgg tacgagctga ggcttatcag
     3781 gttgtaggag ttatggcaga tgcgcttggt gtattcggtg atgcagcagt acagaaagtt
     3841 ctggataacc tgtcacagca aaaacttgtt cacagagatg tgctgccgtt ctcgcttccg
     3901 gtaactccgg atggttggat aagctgtagt gagcgaatgc cgaacgataa acagtatgtt
     3961 tggtgttggg gtaagtctta cggctggact gagtgcgata ccttcgaagg gtattacgat
     4021 tgttcgagaa acaaatggtg ggcagttact gacaatgggg aagaaccggc atcgaaagta
     4081 acccactgga tgccgctgcc agaaccgccg caggaggtga atcaatgagc tggcctgatg
     4141 caatcgtaac tctggggatg gtattcgcag tagcgtttgt tgtgtactcg atttgtcgat
     4201 ggggataacc acatgttcgc tttgattcaa cgcgggcaga tatacaccga tagtgctggc
     4261 tacccgataa aaattgttcg ctgcataaac aacactgtgt tgtacagaag aatggatggg
     4321 cgaacacagt cagtaaaaat aaacgatttc aatgaactgt ttgaacggat tgatcaccaa
     4381 gaataccgac aaattctggc agaaacagag caggaagctc atctgaaaaa attacgagcc
     4441 atgaaaagga agtaaacaat gaataaagca tttgaacgat gggtccacca gcgttacagc
     4501 aatcgctatg atctgacgcg agatgttgac ggtttctact gtcgtgaagt tgtgaagcga
     4561 atgtttgacg tgtggtgcca ctgccgtgga tgaaaatttt atgaggttgg catgcagaca
     4621 atcatctatc agataacccc cagcaaatgg tgtacggaga gagtcctcat tgcatcaaca
     4681 gggctaaagc ctggcaccat tgagcgggca agaagaaagt catggatgca gggaaaagaa
     4741 taccgccatt acgctgtaga aggtgatccg gggcactaca gtgaatgcct gtacaacatc
     4801 gaagaaatta tgcgatggat cgaaaaccag aaacaaccag gtgccaaaaa tgcaagttcc
     4861 ggttaacctg ttaatgctcc tggacgtctg ggaggtttaa tgagtaacgc atcatacccg
     4921 acaggcgttg aaaaccatgg aggatcactc cgtatatggt ttcactataa tggcaaacgt
     4981 gtcagagaaa acctcggtgt tcctgacaca gccaaaaacc ggaagatcgc tggtgaactt
     5041 cgcacttccg tttgttttgc aatcagaatg gggagtttcg actacgccgc gcagttccct
     5101 aattccccta acctgaaaca ctttggtctg ggaaaaagag agataaccgt taaggcactt
     5161 tcggaaaaat ggttggacct taagaaaatt gagatttgtg cgaatgcact taaccgttac
     5221 cagtcagtaa ttaaaaacat gttaccaatg ttaggtgaaa aaaaactggt ttcatccata
     5281 acaaaagagg atttactttt cgtaaggaga gatttgttga ccggttacca aaagctttct
     5341 aatggaaaga cttcttccat aaaagggcgc tcagtggtca cggtaaacta ctatatgaca
     5401 accatagctg gaatgtttca atttgcaaca gataatggtt atacctcagg aaacccattt
     5461 aacggtctgg cccccttaaa aaagtccaag gtaaaaccag atcctctcac ccgtgacgaa
     5521 tttattcgtt ttattgaagc ttgccgtcat caacaaacaa aaaacctgtg gattctcgct
     5581 gtatacacgg gtattcgtca cggggagctg gtatcgctgg catgggaaga tatagatctt
     5641 aaagcaagga ctataaccat ccgtaggaat tatacaaaac ttggcgaatt cactccacca
     5701 aaaaccgatg ctggcaccgg aaggacaatt catctggttc aaccagctat tgatgctctt
     5761 aaaagtcagg cggaaatgac catgcttgga aagcaacatt ctgtagaggt aaagcagagg
     5821 gaatatggga gaagtactgt gcataaatgc acttttgttt ttagtcctca ggtaataaaa
     5881 cagcggcagt tttccggacc gcactataag gttgactcca tcagggagtc atggacaagt
     5941 atcttaaaac gcgcaggtct gagacacaga aaatcgtacc aatccaggca tacttatgca
     6001 tgctggtcac ttgccgctgg agctaatcct agttttatcg caagccagat gggccacaca
     6061 aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag
     6121 atagaactcc ttaacaaaag actatctgaa agtgtcccat gtatgcccca taagaaagct
     6181 gggtaaaata aaaacttgca aaatcaatta gtttaccctt aatccctgtc acgttacgcg
     6241 cgtggcagag gcgttacgga ttgctgaaac cgcaacggac agacggcggt catcggctat
     6301 tcaacgatgc cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc
     6361 aggtcagcaa agtaaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc
     6421 gcgatcagca agaaacatta ctgacttacc tgcaaagcgg caatctgcat agcctgcgaa
     6481 cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta
     6541 ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc
     6601 tcgacggcgt actgatcaac tacatctcta tttgcctggc ttcggcgcgc aaaaaacagg
     6661 gtaaagatgc actggtggtt ggctggaata ttcaggatac cactcgtctg tggctggagg
     6721 gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac
     6781 tacgccctga actgttcgaa ggccgtacat tgctggtatg gtgcggtgat aatcgaacct
     6841 ccgcccaaca gcagcaactc accagttggc aggaacaagg ccatgatatt ttcccactcg
     6901 gcatttaatg attcgttaac aaatacgctt tactgtacaa tcctttcgtt aacataagga
     6961 gtgcattatg cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg
     7021 cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctgaacgcc
     7081 tgcaccagcc tttcaaacag gcggtctgcg atgatcgccg ccagtgccac cagtaacgct
     7141 ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatcgg cgtacccagc
     7201 gtgttggccc ctaccgttga ggcgatcgtc gccgtgccaa tgttgataat caccgaagtt
     7261 cgtacacccg ccagaatcac cggagctgcc agcggtagct cgaccttacg cagtcgctga
     7321 ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc
     7381 ccggcaagtg tcgcctgcag gacgggcagc acgccgtaaa ggatcaaggc gataatcgct
     7441 ggtttcagac caaagccgat caccggaacg gcaattgcca gcaccgcgac gggcggaaaa
     7501 gtctgcccaa cggcggcaat agtttccacc agtgggcgaa attccgcacc ccacgggcga
     7561 gtgacagcaa ttccggcacc agtaccaatg atcaccgcaa acaaactcga aattcccacc
     7621 agccagaaat gagccagtgc cagagctgca aagctttcct gctgataaac gggtcgtggc
     7681 agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagcgcc
     7741 acaaacagag caatcagcca gaacagctga tcgcgcaaca tcttcatacg attacgcctc
     7801 ctccagcaga tcctgaaaat gcagcgtgcc gcaaggctcg ccctgcgtgt tcaccaccgg
     7861 cagcacctcg catccccgcg cgacaaacag agaaagcgca tcgcgtagcg tcatctcttc
     7921 tgccagcgcc tcaccttctg cccgttcttc gcgacgcacg taatccgcca cgctacgtaa
     7981 cgaaagcagg cggacaccca gttcactatg cccaaaaaac tggcggacaa aatcattcgc
     8041 cggatgagtc agcatcgcca gaggattgcc ctgctgtacg acttcaccgt gatccatcaa
     8101 taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatagt
     8161 gcgcccctgc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccac gcgttaccgg
     8221 gtccagcgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg
     8281 cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgcaa
     8341 atttggctcc agccccagta gcgccattaa ttcgtcgata cgatcgtcga tccgcgcccg
     8401 tgaccatttt tgtaattgcg gcacggtggc aatattttgc gccacgctcc agtggggaaa
     8461 caggccaata gattgaatgg catagcccat ccggcggcgc aactccagca ctggcagcga
     8521 gcgaatttct tctccggcaa agcggatcac gccactgtca tgctccacca ggcggttaat
     8581 cattttcaga gtggtggatt taccggagcc tgatgtacca atcagcaccg aaaaactccc
     8641 ctcctgaaaa ttgagattga gatcgttaac ggctttttgt gtgccgaaca gtttgctgac
     8701 atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg
     8761 cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg
     8821 cactgcttaa cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgctg
     8881 caatcaccgc catgccaacg gtttgcacca tcaccacccg caggctgcgc agaaataccg
     8941 gtaacgccag cggcaactga acatgcagga aacgctgcgc cccgctcatc cccatcgctc
     9001 tggcgctctc cagcacatcg cgcgggatct ggttcaagcc gactaccacg ccgcgcacca
     9061 gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta
     9121 tgccgagcgt tcccagccac ggaaaggccg taaccagcgc ggcaagcggc gcaatcaaca
     9181 ggccaaagag cgctaccgaa ggcacggtct gaatgacatt cagcagagaa aaaatcgccc
     9241 cttgccgcgc ggtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag
     9301 gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct
     9361 gacggttggc gtattctttc attaacgaga gatcgttaag cgtgccggag tacagcaacc
     9421 acagcggaac aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga
     9481 ttcggcggat ggcatcgcta caggccagca gtgccagcgc cgcagccagc cagaaaccac
     9541 tgccgaggct ggtacgcgcc agcgcactac cattttgcgc cagttgtgtc gccgcctttc
     9601 cagctcccca caccagcaat acgaagacga attgcgccag aatgagtgca aaaatgctgc
     9661 cttttttagc gggaataaaa caggctgtca gccaggcgca accaacgccc accagcatcc
     9721 agagagtttg cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag
     9781 gtgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca
     9841 gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca
     9901 ggtagtcggc tgccactttt ttggcatcca gtccttccac tgcaatgctg gcattcagct
     9961 gctgcaatgt tttttcatcg aggctggcga agactggccg tagccactgt gccatttgcg
    10021 gatactcctt caacaccgat tcacgcacca ctggtgcagg cgcgtagata ggttgcacgc
    10081 cttgcggatc gcttaaggtt tgcagcccca gcgccgcaac cgggccgtcg gtgccgtaag
    10141 ccattgcggc attaacgcca gaagtttgct gggcagcggc tttgatcgtc accgccgtgt
    10201 caccaccggc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
    10261 acgcgggtaa ggcatcggcg cgttcgataa actctgccga ggctgccagt ttgaagttgc
    10321 caccctcttt cagataacga ctcagctcgg caagcgaagt gagtttgttt ttctctgcca
    10381 catcctgacg cacggcgatg gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
    10441 acttgttttg ctctgcatcg agttttttga ctttctcata gccttgctgc gcgtttttcc
    10501 ataccgtatc gttttcatct ttaaagaaaa acgcgccatt gccagtatat tccggataga
    10561 tatccagctc gccagaagtg atcgccccgc gcactacggg agtcgttccc agctgcactt
    10621 tatttaccgt aggaacgccg tgactttcca gcacctgcaa aatgatattg ccgagcagcg
    10681 cgccttccgt atcgattttt gaaccaacct taacggggga agccgcctgt agcggcaggc
    10741 tcacggctgc caacaaaacc agtgaacctg cccagagctt tgagagtggc atgatgcttt
    10801 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
    10861 ggttagcctg tctggacgga ttcagttgca gaatcagata aatacttaaa gggcatattt
    10921 tgcccggcgt ttcttgccgg atgcggcgta aacgccttat ccggcctaca aacatcgaaa
    10981 attacaacaa ctcaaactcg gctttcttaa cgcgtgcgga atcagtgccg atgaagacat
    11041 tgaacttgcc aggctcggcg tcatatttca tctgttgatt ccagaacttc agcgcctcaa
    11101 tatcgattgg gaagctgacg gtctgagttt cgcccggttt cagggtgatt ttctcaaagc
    11161 ctttaagctg cttcaccggg cgactcatgg aagccgtcac atcctgcaag tacatctgta
    11221 ctaccgtggc cccttcgcgt ttaccggtgt tcgtcacctg cacgctggcc gtcactttgc
    11281 cgtcacgctt catggtcggc gcagaaagtt tcacatcaga gacggtgaaa gtggtgtagc
    11341 tcagaccata gccgaacgga taaagcgccc cgttagcttc atcaaaataa cgcgaagtgt
    11401 atttgttcgg cttgtcggca ttatacggac gcccggtatt cagatggctg tagtacaccg
    11461 ggatctgccc gacagaacgc gggaaggaca tcggcagctt gccggacggg ttataatcgc
    11521 caaacaatac atcggcaatt gcattaccgc cttcagtccc cgcaaaccag gtttccagaa
    11581 tcgcatcagc ctgctgatct tctttcacca gcgccagcgg acgcccgttc atcagcacca
    11641 gcaccagcgg tttaccggtg gctttcagcg ccgcaatcaa gtcacgttgg ctttgcggaa
    11701 tagtgatatc ggtacgactg gaagcttcgt gcgccatccc ctgagcttca ccgaccacag
    11761 ccaccaccac atcagattgt ttcgcggttt gcactgcttc atcaatcatc tcttgcggcg
    11821 aacgcggatc aactttgacc gcctcttcat attgattcag gaaatcgata atgcctttgt
    11881 cactggtaac gttcgcccct ttggcataca gcactttacc gttttcaccg acggcgtttt
    11941 taatcccggt tagcacagta acagattgat cggcgacacc tgccgccgac cagcttccca
    12001 tcacgtcacg cttgctgtct gccagcgggc caaccaccgc aatggttgca gattttttca
    12061 gcggcagcgt ttcgagacga tttttcagca acaccaggct ttcgcgcgcc acttcacgcg
    12121 cttctttgcg gtgcaggcgg ctttcggcat tggtatccac cgggtcagac tctttcggcc
    12181 ccaaatggct gtatgggtcg ttaaacaatc ccatatcata tttaacgttc agtacatggc
    12241 gggcagcatc gtccagctct tccatcgtca ctttgccgga tttgatcaac ccaggcagat
    12301 acttcgagta atactcgtcg ctcatgctca tgttgattcc ggacttcagc gccacgcgta
    12361 ccgcatcttc cgggtctgcc gccgtgccat gtttaatcag ctctttgatt gcgccgtgat
    12421 cggaaacggt gatgccttta aagccccatt ggtcgcgcag aacatctttc agcagccagg
    12481 agtcggaggt ggctggcgtg ccgttcagcg agttcagcgc caccatcacc gcaccgctgc
    12541 ctgcatccag ccccgctttg tacggcggca tatagtcgtt aaacaggcgc tgcggactca
    12601 tatcgacggt gttgtactct ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga
    12661 cgctggtcat caccgagtag cgatctgccg ggcttttacc ctgcatcgct tccaccatgg
    12721 ttttgcccat tattgaggtg agatacgtat cttcgccaaa accttcggaa gcacgtcccc
    12781 agcgcggatc gcgcgagaca tcgaccatcg gtgcccaggt catattcagg ccatcatctg
    12841 ccgcttcata agcagagaca cgcccgactg ttttcaccgc atcgaggtta aaagacgaag
    12901 ccagaccgag actaatcggg aacaccgtgc gctgaccgtg cagcacatcg taagcaaaga
    12961 aaagaggaat tttcaggcgg cttaattcca tcacctgatc ctgcatggcg cggatatcct
    13021 gacgggttac ggtgttgaaa atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg
    13081 cctctttcgg gttatccggg ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa
    13141 ctgtcatttt cttaagcagt tcggtgacga acgcatcccg cgcttcgggc gttaatggat
    13201 ggttgccgaa taaatcatcc gccagtgctg gctgcagggc cagactcacc gcgattccta
    13261 ctgaacatag ccatttcata tggatttttc ttcctttgtt gcccgacgtg gcagcgaaaa
    13321 tggtgcaaaa accgtagttt gccataagca tgatggagag agaaaaagaa tgctcagttt
    13381 attgtctgaa ttttcaaaat attcactcgc tgaattgtca tacaaggcgc tattctagtt
    13441 tgtgatattt tttcgccacc acaaggagtg gaaaatgtct tccatgacaa caactgataa
    13501 taaagccttt ttgaatgaac ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc
    13561 cgcaaaaacg gcccgctatc gcaagggctt ccgttctggt cagggcgacg cgctggctgt
    13621 cgttttccct ggctcactac tggaattgtg gcgggtgctg aaagcctgcg tcaccgctga
    13681 taaaattatt ctgatgcagg ctgccaatac aggcctgacc gaaggatcga cgccaaacgg
    13741 taacgattat gatcgcgata tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt
    13801 actcggtaag ggcgaacagg tgctggccta tccgggcacc acgctctatt cactggaaaa
    13861 agccctcaaa ccgctgggac gcgaaccgca ctcagtgatt ggatcatcgt gtataggcgc
    13921 atcggtcatc ggcggtattt gtaacaactc gggcggctcg ctggtgcaac gtggcccggc
    13981 gtataccgaa atgtcattat tcgcgcgtat aaatgaagat ggcaaactga cgctggtgaa
    14041 ccatctgggg attgatctgg gcgaaacgcc ggagcagatc cttagcaagc tggatgacga
    14101 tcgcatcaaa gatgacgatg tgcgccacga tggtcgtcac ggccacgatt atgactatgt
    14161 ccaccgcgtt cgtgatattg aagccgacac gcccgcacgt tataacgctg atccggatcg
    14221 gttatttgaa tcttctggtt gcgcagggaa gctggccgtc tttgcggtac gtcttgatac
    14281 cttcgaagcg gaaaaaaatc agcaggtgtt ttatatcggc accaaccagc cggaagtgct
    14341 gaccgaaatc cgccgtcata ttctggctaa tttcgaaaat ctgccggttg ccggggaata
    14401 tatgcaccgg gatatctacg atattgcgga aaaatacggc aaagacacct tcctgatgat
    14461 tgataagtta ggcaccgaca agatgccgtt cttctttaat ctcaagggac gcaccgatgc
    14521 gatgctggag aaagtgaaat tcttccgtcc gcattttacc gaccgtgcaa tgcaaaaatt
    14581 cggtcacctg ttccccagcc atttaccgcc gcgcatgaaa aactggcgcg ataaatacga
    14641 gcatcatctg ctgttaaaaa tggcgggcga tggcgtcggt gaagccaaat cgtggctggt
    14701 ggattatttc aaacaggccg agggcgattt ctttgtctgt acgccggagg aaggcagcaa
    14761 agcgttttta caccgtttcg ccgctgcggg cgcagcaatt cgttatcagg ctgtgcattc
    14821 cgatgaagtc gaagacattc tggcgctgga tatcgctctg cggcgtaacg acaccgaatg
    14881 gtatgagcat ttaccgccgg agatcgacag ccagctggtg cacaagctct attacggcca
    14941 ttttatgtgc tatgtcttcc atcaggatta catcgtgaaa aaaggcgtgg atgtgcatgc
    15001 gctgaaagaa cagatgctgg aactgcttca gcagcgtggc gcgcaatacc ctgccgaaca
    15061 taacgtcggt catttgtata aagcaccgga gacgttgcag aagttttatc gcgagaacga
    15121 tccgaccaac agtatgaatc cggggatcgg taaaaccagt aagcggaaaa actggcagga
    15181 agtggagtaa taataacgga tggcagagta tcgccatccg tcattcattt aatcgttctg
    15241 tgccgtctgc cccgccgctg ccatttgggc ggctttttgt tttttatagc tcaacgctgc
    15301 tgccggaaca ggcatcactt taccggtttc aatccaggta cgcaggcggc tggcatcggc
    15361 aaaatgggta tatttgccaa acgcgtccat cactaccagc gccaccggtt tattattgat
    15421 aaccgtgcgc atcaccagac aatggcccgc cgcattggta aaaccggttt tggttaactg
    15481 aatattccag ttatcacgat acaccagatg gttagtattg cggaacggca gcgtatacgt
    15541 cgggttagag aaggttgcca tatcttcccg ggtagtactt aactgcccga tcaacggata
    15601 ttgtttgctg gcaataagta gtttggttaa gtcacgggcg gttgaaacgt tatgcaccga
    15661 caatccggtt ggttcaacaa agcgcgtgtt gttcattccg agcgatttcg ctttcgcatt
    15721 catcgcctta ataaaggctt tgtaaccacc gggataatgg tgcgcaaggc ttgccgccgc
    15781 gcggttttct gaagacatca gcgccagcaa cagcatatct ttacggctga tttcgctgtt
    15841 cagtcgtacg cgcgaataga cccctttcat ctccggcgtc tggctgatat ccacttttag
    15901 tttttcatcc agcggcagtc gtgcatccag cacaaccatc gcggtcatta atttgctgat
    15961 agacgcaatc ggacgcacca gatccgggtg gttcgaatag atcactttgt tggtattcag
    16021 atcaacaatc atcgcgctac cggaggcaat ttccggttgt gacgcggtgg tagcggctgc
    16081 cgttttcgca acggcctgcg gtgcaaaagg cacagccagc atcagggcca ggctaaataa
    16141 agaaactcga aatttcggca tgatgagcat tcagatagtg gttcacgcgc acgggttgcg
    16201 caccgccgga gtaaggattt actgaggcta gcgacgccat cataacgagc aaaaagtgcg
    16261 atcgtcaaag aagaatcgtg aggaaatgct gcattgctga catttacgcc agcaatgcag
    16321 cgcaaaagaa ctttctagaa caaacgataa ccgtagcccc atagtataac ggtaagggcg
    16381 agcagcactt ccagcaccag cacgcctatc gccagtgtcg aactggagaa gctaagtcct
    16441 tcctctttgt tgatattcag gaagctcgga ataccgagat acagcaggta gccggtgtaa
    16501 aacagcgcca cagtgccgac cagcgcacac aaccagacca gcgggtaaag cgccaccaga
    16561 ccgcttaaaa acagcggagt tgcaacgtag cccgcgaaga ccatgcagtg cgcaagtgac
    16621 ggacgctgcg gataattacg cgccatccac cagatgaccc gccccatcac cgcaacccca
    16681 gccagcataa cgccgtaaaa caagacagcc agcgccagtc cggtaaacca ggataacttc
    16741 aggatagtgc catcgccaaa attccagcca atctgtgtag tgccaatgaa ggcgcaaatc
    16801 accggaatcg ccgccatcag caaaacgtgg tgggtgtagt gatgagaaat cgtttcgttt
    16861 tcgcgattaa tcacctgcat ttcacgatcg ggatgggaaa acagtcccca gacatggctc
    16921 ataccgcccc cttgttgtga gttcatgaac ctgacagttc aagtataagt cagcttgtga
    16981 ttattttttg ttcgctcatg taatttgcac cgtctgatgt acgtcctttt tcagcgtgaa
    17041 tgattcacag ggtgtatgct tacagtcaag caaagggaga cagactgtcc tatggatctc
    17101 aatacactca tctcacaata tggttatgcc gcgctggtga tcggtagcct ggcggaaggt
    17161 gaaaccgtga ctttgctggg aggcgttgcg gcacatcagg ggctattaaa gttcccgctg
    17221 gtggtacttt ctgtggcgct tggcggcatg attggcgacc aggtgcttta tctttgcggg
    17281 cggcggtttg gcggcaagct gttacgccgt ttctcgaaac atcaggataa aattgagcgg
    17341 gcgcagaaac ttatccaacg ccatccgtat ctgtttgtca ttggtacgcg ttttatgtat
    17401 ggctttcggg tcattggccc gacgctgatt ggtgccagcc agctgccgcc gaaaatcttt
    17461 ctgccgctga atattctcgg cgcatttgcc tggtcgctga tttttaccac tatcggttac
    17521 gctggtgggc aggtgattgc gccgtggttg cacaatctcg accagcattt gaagcactgg
    17581 gtctggttga ttctggtcgt ggttctggtg gtaggcgtgc gctggtggct gaaacggcgc
    17641 gggaagaaaa agccggatca tcaggcgtaa aactattgcc ctggataagg tgttcgcgcc
    17701 gcatccgaca tccggtgcac catgcctgat gcgacgctgg cgtgtctttt ctggcctaac
    17761 ccaatgaaat aaccacgcac ctcactgcgc tattctgggt tgaactgcgg attcaccaac
    17821 ataaagccgc catccactat cagcgactgc ccggtggtgt aatttgcgcc ctccgaacaa
    17881 agccatacca ccaggctggc aatctcatgc gttgtgccaa aacgtcgcaa gggaatcgaa
    17941 ggctccgcgt cgggcttcac gtcgccgcca tccatgccgt tcattggcgt ggcgatcgcc
    18001 ccaggtgcga ctgcgttcac caaaatttta tgcctgacca actccagcgc cattgctttg
    18061 gttaacccgc cgagcgcatg tttagcggct gtgtaggcgc tggcatcagg cagcggcgta
    18121 tgttcatgta ccgatgtaat gttgatgatg cgaccgccct gcccttgctt caccatctgg
    18181 cgggcagcaa tttgcgagca taaaaatgca ccatcgacat caacggtaaa aatcttgcgc
    18241 cactcatcga aagccatatc aagaaacggc gctttggtca ttgcgcccgc attattcacc
    18301 agcacatcaa tacgccccag ccgttgaata agtttctcca gcgcctgcgc accttctggt
    18361 agattgccga gatccagttg cacgatctcc gcacgtacgc cgtggctaac tacctcacgc
    18421 gcggtatctt ttgccccttc ttcatctgag tgccaggtaa tgccaatatc aaacccctgc
    18481 tgcgccagta ataacgcgca ctctttgccg atccccgaat cggaggcggt aattatcgcg
    18541 acctgtgcca tcgagttctc cacttaacgc tcaacaaacg ttaagtatag aaggcgcata
    18601 tcatcagcgt ttgtaccccc cacccagcgc accagtgagt tgaatggagg catccagcca
    18661 ctgcccttgc aataacaggc catgggcccg ctcacgcagc gccgggattc tggcttcgct
    18721 gacccgggaa ccagcaatga ggcccgcgtt aaagcgcgcc tgcgcaagac ctactacacg
    18781 caaggcatcg cgctcaattt gcgcctgatg ctggtttttc tccgccagtg tctgaacctg
    18841 actggctgcc cgcgccacgt cattcaccgc ttcaaccacc gctttgttgt agctggcgat
    18901 agacaagttg ctttcggctt ttgcgatatc gaggtt
//
LOCUS       DAZGJT010000093        18797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZGJT010000093 DAZGJT010000000
VERSION     DAZGJT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.093"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..113)
                     /locus_tag="ACHKFK_003601"
                     /pseudo
     CDS             complement(1..113)
                     /locus_tag="ACHKFK_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            114..270
                     /locus_tag="ACHKFK_003602"
                     /pseudo
     CDS             114..270
                     /locus_tag="ACHKFK_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tartrate dehydrogenase"
     gene            complement(363..1478)
                     /gene="rnd"
                     /locus_tag="ACHKFK_003603"
     CDS             complement(363..1478)
                     /gene="rnd"
                     /locus_tag="ACHKFK_003603"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO9053854.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQALPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(1560..3245)
                     /gene="fadD"
                     /locus_tag="ACHKFK_003604"
     CDS             complement(1560..3245)
                     /gene="fadD"
                     /locus_tag="ACHKFK_003604"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO9053855.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(3450..4031)
                     /gene="yeaY"
                     /locus_tag="ACHKFK_003605"
     CDS             complement(3450..4031)
                     /gene="yeaY"
                     /locus_tag="ACHKFK_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO9053856.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQNLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(4071..4766)
                     /gene="tsaB"
                     /locus_tag="ACHKFK_003606"
     CDS             complement(4071..4766)
                     /gene="tsaB"
                     /locus_tag="ACHKFK_003606"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO9053857.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLLDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAELVYL
                     RNNVAWKKLPGKE"
     gene            complement(4824..6734)
                     /gene="yoaA"
                     /locus_tag="ACHKFK_003607"
     CDS             complement(4824..6734)
                     /gene="yoaA"
                     /locus_tag="ACHKFK_003607"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO9053858.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIELAESLLLPSPFDYSRQALLYVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            6866..7210
                     /gene="yoaB"
                     /locus_tag="ACHKFK_003608"
     CDS             6866..7210
                     /gene="yoaB"
                     /locus_tag="ACHKFK_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO9053859.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFDQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            7632..7931
                     /gene="yoaC"
                     /locus_tag="ACHKFK_003609"
     CDS             7632..7931
                     /gene="yoaC"
                     /locus_tag="ACHKFK_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO9053860.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITTRADLEGWNPGFTEKMVGWAKKMESGERIVIKNPEYFSTYMQEELKALV"
     gene            complement(8051..8230)
                     /gene="yoaH"
                     /locus_tag="ACHKFK_003610"
     CDS             complement(8051..8230)
                     /gene="yoaH"
                     /locus_tag="ACHKFK_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO9053861.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIMARFEDEDE"
     gene            8304..9665
                     /gene="pabB"
                     /locus_tag="ACHKFK_003611"
     CDS             8304..9665
                     /gene="pabB"
                     /locus_tag="ACHKFK_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO9053862.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPAHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALVVDHQRKTVSLLSHNDV
                     NARRTWLENQQFSPQEDFALTSDWQSNMTRELYGEKFRKVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQAAILSLSPERFILCDNGEIQTRPIKGT
                     LPRLPDLQEDSKQAEKLANSTKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPHRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            9669..10247
                     /gene="nudL"
                     /locus_tag="ACHKFK_003612"
     CDS             9669..10247
                     /gene="nudL"
                     /locus_tag="ACHKFK_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO9053863.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            10431..11795
                     /gene="sdaA"
                     /locus_tag="ACHKFK_003613"
     CDS             10431..11795
                     /gene="sdaA"
                     /locus_tag="ACHKFK_003613"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9053864.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGP
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            11926..13524
                     /gene="pdeD"
                     /locus_tag="ACHKFK_003614"
     CDS             11926..13524
                     /gene="pdeD"
                     /locus_tag="ACHKFK_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO9053865.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTTDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDSRHLLYGIGLVDSLPQPEHNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRSHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(13528..15084)
                     /gene="yoaE"
                     /locus_tag="ACHKFK_003615"
     CDS             complement(13528..15084)
                     /gene="yoaE"
                     /locus_tag="ACHKFK_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO9053866.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(15072..15230)
                     /gene="yoaL"
                     /locus_tag="ACHKFK_003616"
     CDS             complement(15072..15230)
                     /gene="yoaL"
                     /locus_tag="ACHKFK_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO9053867.1"
                     /translation="MDRHRRHFSIRPFNACLSGTLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            15547..16518
                     /gene="manX"
                     /locus_tag="ACHKFK_003617"
     CDS             15547..16518
                     /gene="manX"
                     /locus_tag="ACHKFK_003617"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO9053868.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVITGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            16581..17381
                     /gene="manY"
                     /locus_tag="ACHKFK_003618"
     CDS             16581..17381
                     /gene="manY"
                     /locus_tag="ACHKFK_003618"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO9053869.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            17394..18245
                     /gene="manZ"
                     /locus_tag="ACHKFK_003619"
     CDS             17394..18245
                     /gene="manZ"
                     /locus_tag="ACHKFK_003619"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO9053870.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            18300..18758
                     /gene="yobD"
                     /locus_tag="ACHKFK_003620"
     CDS             18300..18758
                     /gene="yobD"
                     /locus_tag="ACHKFK_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO9053871.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMHRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         4579 a         4888 c         4683 g         4647 t
ORIGIN      
        1 aattctaccg ttatccgcac tttcgctgac gtcgacagct ttgaaacata aacggcccag
       61 acattcactg gctggtaata ttccggtagc acttgtacta aatgaccact ggcgggaaac
      121 agcctggcga catcgatttt tacgtggtcc ggaaaaacac cgaaggcgaa tattcctcgc
      181 caggtggtag agtaaatgga aggaacagag aatgaagtcg tcattcagga atcggtattt
      241 actcgtcgtg gtgtcgagcg cattttgcgt taagctcaaa taagttcatt tgaaccacct
      301 gctgttggat ttgtagtttg ccgaagccgg actgagcgcg cccagtccgg cttcggaaga
      361 ttttactgtg gatattcctg caataaattg tgtaatgctt ccgccatcaa ctcaccacgc
      421 cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg ccagttcagc
      481 agttggttga tttgccgacg cgacgccagc aattcggcgc tgatcttgtg cgtttcgctg
      541 acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataacccgg catgtccatc
      601 aggttaagca tcggctgcgg taaggcctct tctggcaatg cctgcgcttt ttccaccagc
      661 gccagcaacg ttttaccgtg gaagcggatt tcgctaccgg ataagcccag gctgtccagt
      721 tcgcccagac ttcccggcat ataacgcgct accgaccaca aatgctcttc acgcacgaca
      781 aagttcaccg ccagatcgcg ctctcgcgcc ttgcgcaatc gccagtcggc taacagttgc
      841 agacaggcca gttggcgtgt gcgtaattgc caggcattgg tgatatcacg ccaggcgtct
      901 tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc cagcgccgca
      961 ggtagccagc cggaggcctc cgtttctacc ataagctttg cggtgatcgg taacagatac
     1021 cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc cagccagtcg
     1081 gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat ggaagcgaaa
     1141 ccccatgaca tcgggcgtcc gcagaaggca gcaaggattt gcgtgtcaat caagggttgt
     1201 ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc atgaagaaat
     1261 tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt gatcccgaga
     1321 ggatcaatta gcgccagatg ctcaccatca aaaagttgaa ttaaacccag ctgcgggtaa
     1381 taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc acggacggct
     1441 tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa attgtattct
     1501 ctttagtggg cgtcaaaaaa aacgccggat taaccggcgt ctgacgactg acttaacgct
     1561 caggctttat tgtccacttt gccgcgcgct tcgtcacgta attctcgtcg caaaattttt
     1621 ccgacgttag atttcggtaa ctcatcacga aactccacca gcttcggtac tttgtagccc
     1681 gtgagctgac ggcggcaaaa agtcaccagt gactcttcgg taagcgatgg atcttttttc
     1741 actacgaaga ttttcaccgc ttcaccactg gagccagaag gtacgccaac agccgcgact
     1801 tcctgtacgc caggatgctg catgacgaca tcttcaatct cgttgggata gacgttaaaa
     1861 ccggaaacca gaatcatgtc ttttttgcga tcgacaatgc gcaggaatcc ttcttcatcc
     1921 atcaccgcga tgtcgccggt gtgtaaccag ccatttttga tgatttcatc ggtagcatcc
     1981 ggacgctgcc agtaacccag catcacctgc ggtcctttga cacaaagctc acctggttga
     2041 cccggtggta cttcattatc atcatcatcc accagtttgg cttccgtcga cggcaccggt
     2101 aaaccgatgc taccactatg ataatcaata tcatacgggt taacgctgac cagcggcgca
     2161 cactcggtca ggccataacc ttccagcaga tactgtccag tcagtttcac ccaacgttct
     2221 gccaccactt gctgaacagg catccctccg cctgcggaaa gatgcagact ggagaaatcc
     2281 agctgctgga actctttatt gttcagcaac gcattgaaca aggtgttaac gccagtgata
     2341 gcggtaaacg gatatttcgc taactctttt accaaccctg gaatatcgcg cgggttagtg
     2401 ataagcaggt tctgcccacc cagttcgata aacagcaggc agttaatggt cagggcaaaa
     2461 atgtgataca gcggcagcgc cgtcaccacc agctctttgc ccggatgcaa cagcggacca
     2521 taggtcgcgt taacctgttc caggttcgcc agcatattgc ggtgagtcag catcgcgcct
     2581 ttcgccacac cagtggtgcc gccggtgtat tgcaggaaag ctaaatcttc cggcaccagt
     2641 tctggtttga catactgcat ccggtagccg ttgtgcaggg cgctacgaaa tgaaatggca
     2701 tctggcagat ggtatttcgg caccagacgc ttgatgtatt taacaacgaa attgactacc
     2761 gtgccttttg ccgtagatag ctgatcgccc atacgggtca gaataacgtg ctgaacggcg
     2821 gttttatcaa ccactttttc cagagtgtgg gcaaagttag acacgataac aatcgccgat
     2881 gcgccgctat cgttaagctg atgctcaagc tcacgcgggg tatacaacgg gttaacgttt
     2941 acgacgatca tcccggcacg caaaatgcca aacagcgcca ccggatattg caataagtta
     3001 ggcatcatca acgcaacgcg atcgcctttc ttcagcccca acccttgttg caaataagcg
     3061 gcaaacgcgc gactgcgttc ttccagtttg cggaaggtca tcacctcccc catattcaca
     3121 aacgcgggtt gatcggcgta gcgcgcgacc gactgctcaa acatatctac cagagattga
     3181 taacggtcag ggttgatctc cgtcggaacg tccgcgggat aacggttaag ccaaaccttc
     3241 ttcaattctt cacctctaaa atgcgtgttc gtcgtcatcg caaccccaag taataaacat
     3301 atcgttaaca taatattaac tcatcatacc agcttgataa ttacccaacg aaaaggttgc
     3361 gaagcgcgtc actatttatt tttatcttta ccgtaagaat gcagaaacag cggaccggcc
     3421 gttgtttctt tttctttaaa aacaagcaat tattcagtta caactgtttg tactctcgcg
     3481 gggccgggat tataccagcc ccatccgccg tagccatagg gccagccacg accgccataa
     3541 aaccatggat caatcggctg aggcggcata atcacctgct gggttaaatg ccaacgtttg
     3601 taacccgtta cttgcatcac cataaattta tagggcgtat taccgatttt gccgtcaacc
     3661 gcaccggtga ttggcccgac taccgtcacc agttgtccgc ggaaatccac cgggtccaga
     3721 aaaccgttca catcggcata aatgcgaccg cgagaaggtt ctcccagcgt cggtctggct
     3781 ccgctgtcca gtggtacggt ggcaatttcc aggcgggttt tcccttgctg gttttgtacc
     3841 gcaaccactt tgccgccaaa gcgtgcctcc tgaccaacgt acagctgcgg cgcactcatc
     3901 acccgaacta aattttgttg cggcgtggga ctgctgcctt taatggcgtc cggcacagtg
     3961 acacaaccgc tcagcattag cgcaaacgta cctgccagta tgcctttgat aacattcttt
     4021 tgaaccgcca tgatgcgact cctttttttc tcagggcata ctcttaagat tcattctttg
     4081 cccggaagtt tcttccatgc gacgttgtta cgtaaataaa ctagttcagc atgttccacc
     4141 gccaccgttt taccctcggc aaacatctgg cacgcaatcg gcagcatatc ttctgcagcg
     4201 ggcagtaaca cttcgccatc gagcaaaacc agcccgctct ctttaccgag atccggccag
     4261 gcttgccagc ccgtccctac tgtcacccat tcgccggaaa gctgttgcat tcgttcatga
     4321 acgagttcgg gtttgagtac ggcttcggtt tcttcaccgt gccagatacc gttttcatca
     4381 cgctgatatt cggcccagta aacttcgccc attcgcgcgt caatagctgc cagcacgcgg
     4441 gttgcgccgt ttttgcgcca cgccccttgc gccatcgtca ttagcgtgga gacgccaatc
     4501 attggtaact ccgcgccaag tgccagtcct tgcgcaatgc caataccaat gcgcacgccg
     4561 gtgaagctac cggggccgcg cccgtaagcc agagcgttta tgtcagtcag ggaagttccg
     4621 ctggtggtca ggatatcctg caccatcggt aagattcgtt gagtatgttc acgagggcaa
     4681 agctcaaaat gagcgttgac agtaccgtcg ttccacaggg cgacagagca ggcctctgtc
     4741 gcggtatcga tagccagaat tcgcatgggt cttcgtgctt agatcaataa aaaggcgcgc
     4801 atcataccat actccgtaac aaattacctg gaggatggta tcgcaaggaa acgaaccgca
     4861 cgggcaatgt cacgggtgcg cggcgctggc ggcagactgg cgagaaacgt cgcgccgtaa
     4921 gggcgcatca ccagccgatt gtcgcaaatc actaacacgc cgcgatcgtc ggcgtcgcga
     4981 atcaatcgcc ctaccccctg cttgagagta atgaccgcat ccggcagttg tacttcatcg
     5041 aacggatcac caccgcgcaa acggcaatct tccatgcgtg cttttaatag tggatcatcc
     5101 ggcgaggtaa acggcaattt gtcgataatc accaatgaca atgtatcgcc acgcacgtcc
     5161 accccttccc agaaactgct ggttgccaca agaagcgcgt taccggcgct gacaaattgc
     5221 tgcaacagtt gccctttgct ggtttccccc tgcaacaaaa cgggaagcgt catggtagcg
     5281 cggaactgct cggccagatc gcgcatcatg gcgtgcgagg tacaaagcat aaaacaacga
     5341 ccgttgttag cttcgatgat cggtcgcagc attgccgcca gttgccgtgc ggaacccggt
     5401 tgattagttt gcggcaggtt gcgcggcaca tagagtaacg cctggcggct gtaatcaaag
     5461 gggctgggta acagcaacga ctctgccagt tcaatgccaa gccgcgaggt gaaatgatgc
     5521 agatcgtcgt tcaccgacag cgttgctgag gtaaagatcc agctaccggg tttttgcgcc
     5581 attaactctt tgaatttatc cgccacgctg agcggcgtga gagccagagt aaaatggcgc
     5641 gaagtgcatt cgtaccagta gctgtagccc ggctgattga tctcttttag ccgtttcagc
     5701 cgtgtgcgat ataacgtggc gcgttcaaat gccgcatcca gcaaggcgga acgccccagc
     5761 gacagtttcg ccacgtcata acaaagttcc agagtgtcat cgagcagtaa aaatgcccgc
     5821 tgaatttgcg gattagctaa cagctcacgc aggttaccgc gataacctgg ctcaccgagt
     5881 tgcaggcgaa aatcctgcgc actctgggca agacggtcag cgcacttttg taactgctgg
     5941 gtgtctttta attcggtgcg gtaggcgatg gtgatgtctt ttgccaggtc gagcagttgt
     6001 cgactggaga gtgactgacc aaaatactgg ctggcaatgt cgggcagctg gtgggcttcg
     6061 tcgaagatca tgacgtcagc ttccgggatc agttcgccaa atccactctc tttcaccacc
     6121 atatccgcca gaaagagatg atggtttacc accaccacat cggcgtccat cgcttttttg
     6181 cgtgctttga ctacaaagca atctttatac atcgggcagt cgctgccaag gcagttatcg
     6241 ttggtgctgg tgaccagcgg ccacgcctgt gaatcttccg ccacgctgac gcaggtgctg
     6301 atatcaccat cgactgtttg attagaccag gagcgcagca ggatcacatc gcttaatatt
     6361 tgtaccggca gatcgccgcc cgctagcgcc tgttgttcga gacgttcgag gcagaggtag
     6421 tttgagcgcc ctttcagcaa cgccacgttg cccgtatatt tcaacgcctt tgaaaccgtt
     6481 ggcaaatcgc ggctgtagag ctgatcctgc aacgcttttg aaccggtcga gataatgact
     6541 ttctttttcg cccgcagcgc aggggccagg taagcgtagg ttttgcccgt accggttccg
     6601 gcttccacca ccagcggctg gcctttttct atcgcctggg tgacggctac cgccatctgt
     6661 cgctgtggtt ctcgcggctt aaagcctggt atcgctttcg ccaactgacc gtctggtgca
     6721 aaatcgtccg tcacactacc ccctgttgat ttgaacaggg attatgtcag gatgagggcg
     6781 ctttcgccag ttgaagtggt gacggcgacc tcacattgtg gcagtctttg cagcacgaaa
     6841 atggaaggat aatgaggaca aaatgatgac tatcgttcgt atcgatgctg aagcccgctg
     6901 gtctgatgtg gtgatccaca acaacacgct ctactacact ggtgtaccgg aaaacctcga
     6961 cgccgatgct tttgaccaaa ccgccaacac gctggcgcag attgacgccg tgctggaaaa
     7021 acagggcagc aataaatcga gcattctgga tgccaccatt ttcctggctg ataaaaacga
     7081 cttcgcggcg atgaataaag cgtgggatgc ctgggttgtc gcgggtcatg cgccggtgcg
     7141 ctgcacggta caagcgggtt tgatgaaccc gaagtataaa gttgaaatta agattgtggc
     7201 tgcggtgtaa gctttatcga agcaaaataa gtcagacgat aatttatcga taatactggt
     7261 cggttttaca taaatcgacc agagattaag atattactgt tgatgctcct gctaattcat
     7321 tatttatttt tataatttca attttagcta taaaactaac tatataaata aacataacaa
     7381 tgccgttgtg ttcgcccttc gattagcgtc tctggttttc tttcttagct gaacaatttg
     7441 gttatagcgt gagaactgac cgttaatttt ctccctctta ctggataaca caccatgagc
     7501 tgctaccttt aaaacacctg aatgtcaaca ggttaactcg cgccatatcg ttataatccc
     7561 tttgatatta gatgcaaatt aaggtcatat atagccttat cgataacatg gttaatttta
     7621 aggataagaa tatgcctgct gtaatagata aagccctgga tttcattggt gccatggatg
     7681 tatcagcacc aacaccaagt tcgatgaatg aaagtacggc gaagggaatc tttaaatatt
     7741 taaaagaact gggagtaccc gcgagtgccg ctgatattac gacgcgagct gacctggaag
     7801 gctggaaccc ggggttcacg gaaaaaatgg ttggatgggc aaaaaaaatg gagtctggtg
     7861 aacgcattgt gattaaaaat cctgaatact tttcaacata tatgcaggaa gaactaaaag
     7921 cactggtcta agttaaattt acatcagcat aaatgggtca ggacgctttt aatcacatat
     7981 gaaaagcgtc ctttttctca caatcaaata accatcggct tctataacga aagcgatacg
     8041 gataagtgtg ttactcgtct tcatcctcaa aacgcgccat gattcgctcg ccggaatggt
     8101 tggcacgcag ctcttctgcc accagcgcga ttgcctggcc gctgctcatc ccttgagcca
     8161 tcagttcctg gatccgctcg acggcttttt gctgctgttc atgggtgagt gaaggtaaac
     8221 ctgcaaacat tgttaactcc tgctaaattg ttggcgctaa ttatttcatg ctacccggca
     8281 catagccagt agagtcagga ctgatgaaga cgttatctcc cgctgtgatt actttaccct
     8341 ggcgtcagga cgccgctgaa ttttatttct cacgcttaag ccacctgccg tgggcgatgc
     8401 ttttacactc cggctatgcc gatcatccat atagccgctt tgatattgtg gtcgccgagc
     8461 cgatttgcac tttaaccact ttcggtaaag aaaccgttgt tagtgaaagc gaaaaacgca
     8521 caacgaccac tgatgacccg ctacaggtgc tccagcaggt gctggatcgc gcagacattc
     8581 gcccagcgca taacgaagat ttgccatttc agggcggcgc gctggggttg tttggctacg
     8641 atctgggccg ccgttttgag tcactgccag aaattgcgga acaagatatc gttctgccgg
     8701 atatggcagt gggtatctac gactgggcgc tggttgtcga ccaccagcgt aaaacagttt
     8761 ctttgctgag tcataatgat gtcaatgccc gtcggacctg gctggaaaac cagcaattct
     8821 ccccgcagga agatttcgcg cttacttccg actggcaatc caatatgacc cgcgagctgt
     8881 acggcgaaaa atttcgcaaa gtacaggaat atctgcacag cggtgattgc tatcaggtga
     8941 atctcgccca gcgttttcat gcgacctatt ctggcgatga atggcaggca ttccttcagc
     9001 ttaatcaggc caatcgcgcg ccgtttagcg cttttttacg tcttgaacag gctgctattt
     9061 taagcctttc accagagcgg tttattcttt gtgataatgg tgaaatccag acccgcccga
     9121 ttaaaggcac gctaccacgc ctgcccgatc ttcaggaaga tagcaaacaa gcagaaaaac
     9181 tggcgaactc aacgaaagat cgtgccgaaa atctgatgat tgtcgattta atgcgtaatg
     9241 atatcggtcg tgttgccgta gcaggttcgg taaaagtacc agagctgttc gtggtggaac
     9301 ccttccctgc cgtgcatcat ctggtcagca ccataacggc gcaactacca gaacagttac
     9361 acgccagcga tctgttgcgc gcggcttttc ctggtggctc aataaccggg gcaccgaaag
     9421 tacgggctat ggaaattatc gacgaactgg aaccgcaccg acgcaatgcc tggtgcggca
     9481 gcattggcta tttgagcttt tgcggcaaca tggacaccag cattactatc cgcacgctga
     9541 ctgccattaa cggacaaatt tactgctctg cgggcggtgg aattgtcgcc gatagccagg
     9601 aagaagcgga atatcaggaa acttttgata aagttaataa gatattacgc caactggaga
     9661 agtaagacgt ggaataccgt agcctgacgc ttgatgattt tttatcgcgc tttcaacttt
     9721 tgcgcccgca aattaaccgg gaaaccctaa atcatcgtca ggctgccgtg ttaatcccca
     9781 tcgtccgtcg accgcaaccg gggttgttgc tgactcagcg ttcgattcat ctgcgtaaac
     9841 acgctggaca agtggcattc cctggaggcg cagtcgatga cacggacgca tccgttatcg
     9901 ccgccgcgct gcgcgaagct gaagaagagg tcgctatacc gccttccgcc gttgaagtta
     9961 tcggcgtgct gccgcccgtc gatagcgtaa ctggctacca ggtaaccccg gtggtcggca
    10021 ttatcccgcc cgatctgccg tatcgcgcca gtgaagatga agtctcggca gtgtttgaaa
    10081 tgccgctcgc ccaggcatta catctgggtc gttatcaccc tttagatatc taccgccgtg
    10141 gcgattcaca tcgggtatgg ctatcctggt acgaacagta ttttgtatgg ggaatgaccg
    10201 caggcataat tcgtgaactg gcgctgcaaa ttggtgtgaa accctgacta tacttatctt
    10261 tacatctaca aaacactact tgagacaatc atcgcaatat tagttaaatc gcggtttttg
    10321 attagtttaa ttcatgtgaa tagttaagcc agtcgccgcg ttccctctta cactatgcgc
    10381 tgttattagt tcgttactgg aagtccagtc accttgtcag gagtattatc gtgattagtc
    10441 tattcgacat gtttaaggtg gggattggtc cctcatcttc ccataccgta gggcctatga
    10501 aggcgggtaa acagttcgtc gatgatctgg tcgaaaaagg cttactggat agcgttactc
    10561 gcgttgccgt ggacgtttat ggttcactgt cgctgacggg taaaggccac cacaccgata
    10621 tcgccattat tatgggtctt gcaggtaacg aacctgccac cgtggatatc gacagtattc
    10681 ccggttttat tcgcgacgta gaagagcgcg aacgtctgct gctggcacag ggaccgcatg
    10741 aagtggattt cccgcgcgac aacgggatgc gttttcataa cggtaacctg ccgctgcatg
    10801 aaaacggtat gcaaatccac gcctataacg gcgatgaagt cgtctacagc aaaacttatt
    10861 attccatcgg cggcggtttt atcgtcgatg aagaacactt tggtcaggat gctgccaacg
    10921 aagtgagcgt gccgtatccg ttcaaatctg ccaccgaact tctcgcgtac tgtaatgaaa
    10981 ccggctattc gctgtctggt ctcgctatgc aaaacgaact ggcactgcac agcaagaaag
    11041 agatcgacga gtatttcgcg catgtctggc aaaccatgca ggcatgtatc gatcgcggga
    11101 tgaataccga aggcgtactg ccaggcccgc tgcgcgtgcc acgtcgtgcg tcggccctgc
    11161 gccggatgct ggtttccagc gataaactgt ctaacgatcc gatgaatgtc attgactggg
    11221 taaacatgtt tgcgctggca gttaacgaag aaaacgccgc cggtggccgt gtggtaactg
    11281 cgccaactaa cggtgcctgc ggtatcgttc cggcagtgct ggcatactat gaccacttta
    11341 ttgaatcagt tagcccagac atctataccc gttactttat ggcagcgggc gcgattggtg
    11401 cactgtataa aatgaacgcg tctatttccg gtgcggaagt cggttgccag ggcgaagtag
    11461 gtgttgcctg ttcaatggct gctgcaggcc ttgccgagct gttgggcggt agcccggaac
    11521 aggtttgtgt ggcagcggaa attggcatgg agcacaacct tggtttgacc tgcgaccccg
    11581 ttgcagggca agttcaggtg ccgtgcattg aacgtaatgc cattgcctct gtgaaggcga
    11641 ttaacgccgc acggatggct ctgcgccgca ccagtgcacc gcgcgtctcg ctggataagg
    11701 tcatcgaaac gatgtacgaa accggtaagg acatgaacgc taaataccgc gaaacctcac
    11761 gcggtggtct ggcaatcaaa gtccagtgtg actaatactt tatcctcgcc catctgcaac
    11821 ggatgggcga atttatcccc gctttctcgt ctgctgtaat tttccccact acacttccac
    11881 tgttgcgtca ggcgtttgtc gccatacgct tacagggtgg cccgcatgca aaaagcacaa
    11941 cggatcatta aaacctatcg ccgtaatcga atgattgttt gtacgatttg cgcactcgtt
    12001 acgctcgctt cgaccctgag cgtgcgattt atttcacagc gtaacttaaa tcaacaacgg
    12061 gtagtacaat tcgccaatca cgctgtagag gaattagata aagtactgct tcccctacag
    12121 gcaggtagcg aagtcttact tccgctaatt ggtctgccct gctctgtcgc ccatttgcca
    12181 ttacgtaaac aggctgcaaa actccaaact gtgcgatcca ttggcctggt gcaagacggc
    12241 acactttatt gctccagcat ttttggttat cgcaatgtgc ccgtcgtgga cattctggct
    12301 gaacttcctg caccgcaacc acttttacga ctgacgaccg accgtgccct gataaaaggc
    12361 agtccggttt tgattcaatg gacgcctgca gcgggcagta gcaatgctgg ggtcatggag
    12421 atgattaaca tcgacttact gacggcaatg ctccttgaac cacaactgcc gcaaatcagt
    12481 agcgccagcc tgacggtgga cagtcggcat ttgctctatg gtattgggct ggtagattcc
    12541 cttccgcaac ctgaacacaa tgaaaactat caggtttctt cgcaacgctt tccttttacc
    12601 attaacgtta atggcccggg ggctacggcg ctggcatggc actatcttcc aacacaatta
    12661 ccgctggcgg tgctgctaag tttactggtg ggctacatcg cctggctggc gaccgcttac
    12721 cgaatgagct tttcccgcga gatcaatctg ggcctggcgc aacatgagtt cgaattgttc
    12781 tgtcagcctt tgcttaatgc gcgcagccag caatgtattg gtgtagagat tttgctgcgc
    12841 tggaacaatc cgcgtcaggg ctggatttca ccggatgtgt ttattcctat cgcggaagaa
    12901 catcatttaa ttgtgccact aacccgctat gtgatggcag aaaccattcg tcagcgccat
    12961 gttttcccaa tgagtagtca gtttcatgtt ggcattaacg tcgcacccag ccattttcgc
    13021 cgtggtgtgc tgataaaaga tctcaatcag tactggttta gcgctcaccc gattcagcaa
    13081 ctgatcctcg aaatcaccga acgcgatgcc ttactggatg ttgattaccg gattgcccgc
    13141 gagctgcatc gtaaaaacgt caaactggcg attgatgact tcggcaccgg caacagttca
    13201 ttttcctggc ttgaaacatt acgtcctgac gttctgaaaa ttgataagtc atttaccgca
    13261 gctataggtt ctgatgcggt taactcgacg gtgaccgata tcatcatcgc actggggcaa
    13321 agactgaata ttgaactggt ggcggagggc gtagaaacgc aagaacaggc gaagtatttg
    13381 cgcagtcatg gcgtgcatat tttgcaaggg tatttgtacg cacagccgat gccgctacgt
    13441 gattttccca aatggctggc gggcagccaa ccgccgcccg cccggcataa tggacatatc
    13501 acacccatta tgccgttacg ttaacgctta ctcatcttca tcgtgcgccg gttgctcttt
    13561 aacaatgcga accagatcaa cacgataatc attggcttca atgatggtga tatgcagtgg
    13621 ccctacatca atcacatcgc ccacacgggg aatgtgacca tttgccgaga tcacgaggcc
    13681 cgcgaccgtc gcgatatcat cgtcatcggc aaggtgctca acatcaagcg cctgctgcaa
    13741 ggcatgcaaa tctgtaccgc cttttaccag ccagccgtca ccgtcggtaa tgatttccgg
    13801 cgtttcgtca gcgtccggga attcacccgc aatggcttcc agcacatcca gcggcgtgac
    13861 cagaccttgt accacaccaa actcgttggt cacaataaca aagctcccgc gagcacgacg
    13921 cagcacgccc agcaggttga tcggatcgag ggtttccggg acgataatcg ccggagacgc
    13981 cgaagcaatc gccgccacat caacgccctc ttccagcgcc accagcagtt ctttagcacg
    14041 tacgatgccg ataatttcat ccagttcacc gcgacatacc gggaacaaac tgtgcggtga
    14101 ggagagcagc tgctcgcgga tttcatcgac cccgagatta gcatcaaccc agcttatttc
    14161 accgcgcggc gtcatgatcc cgcgcagaga acgcgacgcc agcgtcagta cgccgttaat
    14221 catgtaacgt tcttcttcgg caaacgcacc ttccgggatc ggcatcggca tcgggttatc
    14281 ggcatcgtgc tgaacattgg cctgacgttt cccgcccatc aaacgcagga tggcatcggc
    14341 agtacgcgct cgcagcggca aagtcgactg gtgacgaata aagttgcgac gcgcaatctg
    14401 gttaaacact tcgatgatga tcgagaagcc aatcgcggca tacaggtaac ctttcggaat
    14461 gtggaaaccg aaaccttctg ccaccagact cagaccaatc attaacagga agctcagaca
    14521 gagcaccacc accgtggggt gctggttaac gaatcgcgtc agcggttttg atgccagcag
    14581 cataaccgcc atcgcaatca ctaccgccgc catcatcacc ggcaaatggt taaccatccc
    14641 tactgcagta attaccgcat ccaacgagaa gacggcgtca aggatgacaa tctgtgtgac
    14701 gaccacccag aaactggcgt agcctttacc gtggccggaa tcatgatcgc ggttttccag
    14761 ccgttcatgc agttcggttg ttgctttgaa cagcaagaat atgcccccga acaacataat
    14821 caggtcgcgt ccggagaagg agaaatccat gacggtaaat agcggtttgg tcagcgtgac
    14881 catccatgaa atcagcgaca gcagccccag acgcataatc agcgccagtg ataatcccag
    14941 caaacgcgct ttatcgcgtt gttttggcgg cagtttgtca gcaagaatgg cgatgaagac
    15001 caggttatcg atacccagca caatttcgag aacaacaagc gtgagtagcc ccgcccaaat
    15061 cgaggggtcc attaagaatt ccatgacaag ctcctgctta aggaatagct attcgacgcc
    15121 agaaataatg caggcgtaac gacaaaatgc aaacgaaagg tgcggcataa agtgccagaa
    15181 aggcaggcgt taaaaggcct gatgctgaaa tgacgtcggt gacgatccat actgcgggct
    15241 actgccctat actccatggt tgttaaacag gagttaaaca tatcagagac gcctctgatt
    15301 tggcaaagat ttaccttcct ttgcaaacga atgtgacaag gatattttac ctttcgaaat
    15361 ttctgctaat cgaaagttaa attacggatc ttcatcacat aaaataattt tttcgatatc
    15421 taaaataaat cgcgaaacgc aggggttttt ggttgtagcc cttatctgaa tcgattcgat
    15481 tgtggacgac gattcaaaaa tacatctggc acgttgaggt gttaacgata ataaaggagg
    15541 tagcaagtga ccattgctat tgttataggc acacatggtt gggctgcaga gcagttgctt
    15601 aaaacggcag aaatgctgtt aggcgagcag gaaaacgtcg gctggatcga tttcgttcca
    15661 ggtgaaaatg ccgaaacgct gattgaaaag tacaacgctc agttggcaaa actcgacacc
    15721 actaaaggcg tgctgtttct cgttgataca tggggaggca gcccattcaa tgctgccagc
    15781 cgcattgtcg tcgacaaaga gcattatgaa gtcattacag gcgttaacat tccaatgctc
    15841 gtggaaacgt taatggcccg tgatgatgac ccaagctttg atgaactggt tgcgctggca
    15901 gtagaaacag gccgtgaagg cgtgaaagct ctgaaagcca aaccggttga aaaagccgcg
    15961 ccagcacccg ctgccgcagc accaaaagcg gctccaactc cggcaaaacc aatgggacca
    16021 aacgactaca tggttattgg ccttgcgcgt atcgacgacc gtctgattca cggtcaggtc
    16081 gccacccgct ggaccaaaga aaccaatgtc tcccgtatta ttgttgttag tgatgaagtg
    16141 gctgcggata ccgttcgtaa gacactgctc acccaggttg cacctccggg cgtaacagca
    16201 cacgtagttg atgttgccaa aatgattcgc gtctacaaca acccgaaata tgctggcgaa
    16261 cgcgtaatgc tgttatttac caacccaaca gatgtagagc gtctcgttga aggcggcgtg
    16321 aaaatcacct ctgttaacgt cggtggtatg gcattccgtc agggtaaaac ccaggtgaat
    16381 aacgcggttt cggttgatga aaaagatatc gaggcgttca agaaactgaa tgcgcgcggt
    16441 attgagctgg aagtccgtaa ggtttccacc gatccgaaac tgaaaatgat ggatctgatc
    16501 agcaaaatcg ataagtaacg tattgtgttg attatcactc agttttcaca cttaagtctt
    16561 acgtaaacag gagaagtaca atggagatta ccactcttca aattgtgctg gtatttatcg
    16621 tagcctgtat cgcaggtatg ggatcaatcc tcgatgaatt tcagtttcac cgtccgctaa
    16681 tcgcgtgtac cctggtgggt atcgttcttg gagatatgaa aaccggtatt attatcggtg
    16741 gtacgctgga aatgatcgcg ctgggctgga tgaacatcgg tgctgccgtt gcgcctgacg
    16801 ccgccctggc ttctatcatt tctaccattc tggttatcgc aggtcatcag agcattggtg
    16861 caggtatcgc actggcaatc cctctggccg ctgcaggcca ggtactgacc atcatcgttc
    16921 gtactattac cgttgctttc cagcacgctg cggataaggc tgctgataac ggcaacctga
    16981 cagcaatttc atggatccac gtttcttctc tgttcctgca agcaatgcgt gtggctattc
    17041 cggccgtcat cgttgcgctg tctgttggta ccagcgaagt acagaacatg ctgaatgcga
    17101 ttccggaagt ggtgaccaat ggtctgaata tcgccggtgg catgatcgtg gtggttggtt
    17161 atgcgatggt tatcaacatg atgcgtgctg gctacctgat gccgttcttc tacctcggct
    17221 tcgtaaccgc agcattcacc aactttaacc tggttgctct gggtgtgatt ggtactgtta
    17281 tggcagtgct ctacatccaa cttagcccga aatacaaccg cgtagccggt gcgcctgctc
    17341 aggctgctgg taacaacgat ctcgataacg aactggacta acaggtgagc gaaatggttg
    17401 atacaactca aactaccacc gagaaaaaac tcactcaaag tgatattcgt ggcgtcttcc
    17461 tgcgttctaa cctctttcag ggttcatgga acttcgaacg tatgcaggca ctgggtttct
    17521 gcttctctat ggtaccggca attcgtcgcc tctaccctga gaacaacgaa gctcgtaaac
    17581 aagctattcg ccgtcacctg gagttcttta acacccagcc attcgtggct gcgccgattc
    17641 tcggcgtaac cctggcactg gaagaacagc gtgctaatgg cgcagagatc gatgacggtg
    17701 ctatcaacgg tatcaaagtc ggtttgatgg ggccgctggc tggtgtaggc gacccgatct
    17761 tctggggaac ggtacgtccg gtatttgcag cgctgggggc tggtatcgcg atgagcggca
    17821 gcctgttagg tccgctgctg ttcttcatcc tgtttaacct ggtgcgtctg gcaacccgtt
    17881 actacggcgt agcgtatggt tactccaaag gtatcgatat cgttaaagat atgggtggtg
    17941 gcttcctgca aaaactgacg gaaggggcgt ctatcctcgg cctgtttgtc atgggggcat
    18001 tggttaataa gtggacacat gtcaacatcc cgctggtggt ctctcgcatt actgaccaga
    18061 cgggcaaaga acacgttacc actgtccaga ctattctgga ccagttaatg ccaggccttg
    18121 tgccactgct gctgaccttt gcttgtatgt ggctgttgcg caaaaaagtt aacccgctgt
    18181 ggatcatcgt tggcttcttc gtcatcggta tcgctggtta cgcttgcggc ctgctgggac
    18241 tgtaagactg ttgtacacta ccggggcctt ttggccccgt ttttttatct ggaggattaa
    18301 tgacaatcac ggacctggta ctgattcttt tcatcgccgc acttctggcc ttcgcgatct
    18361 acgatcagtt catcatgcac cgccgtaacg gccccaccct gctggcaatt cctttgctcc
    18421 ggcgtggtcg catcgatagc gttatcttcg tcggattgat tgtcattctt atctataaca
    18481 acgtcacgaa tcatggcgcg cttataacaa catggttatt aagtgcactg gctctgatgg
    18541 gtttttatat attctggatt cgcgttccga agatcatctt taaacaaaaa ggttttttct
    18601 tcgccaatgt ctggattgaa tatagccgaa tcaaagcgat gaacttgtcg gaagatggcg
    18661 tgttggtgat gcaattagaa cagcgtcggc tgttaatccg cgttcgaaat atcgacgatt
    18721 tggaaaaaat ttataagctt ctcgtttcaa ctcaataagt tatgaattta gccaaagcta
    18781 tgtttagtgt attttta
//
LOCUS       DAZGJT010000094        18542 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZGJT010000094 DAZGJT010000000
VERSION     DAZGJT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18542
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.094"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..1190
                     /gene="arnB"
                     /locus_tag="ACHKFK_003621"
     CDS             51..1190
                     /gene="arnB"
                     /locus_tag="ACHKFK_003621"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO9053872.1"
                     /translation="MSGFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIEAAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     HTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALKQLAGQ"
     gene            1194..2162
                     /gene="arnC"
                     /locus_tag="ACHKFK_003622"
     CDS             1194..2162
                     /gene="arnC"
                     /locus_tag="ACHKFK_003622"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO9053873.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTAACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAEGSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2162..4144
                     /gene="arnA"
                     /locus_tag="ACHKFK_003623"
     CDS             2162..4144
                     /gene="arnA"
                     /locus_tag="ACHKFK_003623"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO9053874.1"
                     /translation="MKTVVFAYHDMGCLGIEALLSAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPRGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACAARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFMNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPPRHYFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4141..5031
                     /gene="arnD"
                     /locus_tag="ACHKFK_003624"
     CDS             4141..5031
                     /gene="arnD"
                     /locus_tag="ACHKFK_003624"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO9053875.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQAHSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGTPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRIQRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSH"
     gene            5031..6683
                     /gene="arnT"
                     /locus_tag="ACHKFK_003625"
     CDS             5031..6683
                     /gene="arnT"
                     /locus_tag="ACHKFK_003625"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO9053876.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYLPVIIAGSLPWLGLLP
                     GALYAGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADVIDRQERLVLIQYRPK"
     gene            6680..7015
                     /gene="arnE"
                     /locus_tag="ACHKFK_003626"
     CDS             6680..7015
                     /gene="arnE"
                     /locus_tag="ACHKFK_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO9053877.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFAAVNKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSLRHWCGLAFIIG
                     GIVILGSTV"
     gene            7015..7401
                     /gene="arnF"
                     /locus_tag="ACHKFK_003627"
     CDS             7015..7401
                     /gene="arnF"
                     /locus_tag="ACHKFK_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO9053878.1"
                     /translation="MGLMWGLFSVIIASAAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7395..7661)
                     /gene="pmrD"
                     /locus_tag="ACHKFK_003628"
     CDS             complement(7395..7661)
                     /gene="pmrD"
                     /locus_tag="ACHKFK_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO9053879.1"
                     /translation="MEWLVKKTCCNKQDNRHVIMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASCYSPDEWERQCKAAGKTQ"
     gene            complement(7771..9126)
                     /gene="menE"
                     /locus_tag="ACHKFK_003629"
     CDS             complement(7771..9126)
                     /gene="menE"
                     /locus_tag="ACHKFK_003629"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO9053880.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASSF
                     AVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPEGENTFPALASLHIQLVEGAHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     VSAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNDEVWLRAASMAEGYWRNGQRVPLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQSVDLDEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9123..10085)
                     /gene="menC"
                     /locus_tag="ACHKFK_003630"
     CDS             complement(9123..10085)
                     /gene="menC"
                     /locus_tag="ACHKFK_003630"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO9053881.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEDAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWIPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLDKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDVDALERLL"
     gene            complement(10085..10942)
                     /gene="menB"
                     /locus_tag="ACHKFK_003631"
     CDS             complement(10085..10942)
                     /gene="menB"
                     /locus_tag="ACHKFK_003631"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO9053882.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10957..11715)
                     /gene="menH"
                     /locus_tag="ACHKFK_003632"
     CDS             complement(10957..11715)
                     /gene="menH"
                     /locus_tag="ACHKFK_003632"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO9053883.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEVFADYSRLY
                     VDLPGHGGSATISVDGFDDVTGLLCKTLVSYNILNFWLVGYSLGGRVAMMAACQELAG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11712..13382)
                     /gene="menD"
                     /locus_tag="ACHKFK_003633"
     CDS             complement(11712..13382)
                     /gene="menD"
                     /locus_tag="ACHKFK_003633"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO9053884.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGISWQQRLGDWWQDDKPWLREAPRRESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPKNERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13471..14766)
                     /gene="menF"
                     /locus_tag="ACHKFK_003634"
     CDS             complement(13471..14766)
                     /gene="menF"
                     /locus_tag="ACHKFK_003634"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO9053885.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQVYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNIVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14845..15150)
                     /gene="elaB"
                     /locus_tag="ACHKFK_003635"
     CDS             complement(14845..15150)
                     /gene="elaB"
                     /locus_tag="ACHKFK_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO9053886.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15205..15666)
                     /locus_tag="ACHKFK_003636"
     CDS             complement(15205..15666)
                     /locus_tag="ACHKFK_003636"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9053887.1"
                     /translation="MIDWQDLHHSDLSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     EGENRHILGWHNGTLVAYARILKSDDELQPVVIGRVIVSEALRGEKIGQQLMSKALES
                     CTRRWPEKPIYLGAQAHLQNFYGQFGFIPVTDIYEEDGIPHIGMAREVIQA"
     gene            15731..16648
                     /gene="rbn"
                     /locus_tag="ACHKFK_003637"
     CDS             15731..16648
                     /gene="rbn"
                     /locus_tag="ACHKFK_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO9053888.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPHGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEIFDDGLRKVTAYPMEHPLECYGYRIEEHDKLGALN
                     AQALKAAGVPPGPLFQELKAGKTIMLDDGRQINGADYLAVPVPGKALAIFGDTGPCDA
                     ALELAKGVDVMVHEATLDMAMEAKANSRGHSSTRQAAALAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFAVFSI"
     gene            complement(16769..18514)
                     /locus_tag="ACHKFK_003638"
     CDS             complement(16769..18514)
                     /locus_tag="ACHKFK_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HFO9053889.1"
                     /translation="MRNKNIIMLLISSLILSGCGPEPEDKESQQQQPSTPSDQQVLVA
                     QQAAIKEVEQSAAAAKAAADAKALAQQEVQQYSDKQTLQGRLQEAPTFARAAKANATH
                     IANPGTARYQQFDDNPVKQVAQNPLVTFSLDVDTGSYANVRRFLNQGLLPPPDAVRVE
                     EVVNYFPSDWDIKDKQSIPASKPIPFAMRYELAPAPWNEQRTLLKVDILAKDRKSKEL
                     PASNLVFLIDTSGSMISDERLPLIQSSLKLLVKELREQDNIAIVTYAGDSRIALPSIS
                     GSHKAEINAAIDSLDAEGSTNGGAGLEMAYQQAAKGFIKGGINRILLATDGDFNVGID
                     DPKSIESMVKKQRESGVTLSTLGVGDSNYNEAMMVRIADVGNGNYSYIDTLSEAQKVL
                     NSEMRQTLITVAKDVKAQIEFNPAWVTEYRQIGYEKRQLRAEDFNNDNVDAGDIGAGK
                     HITLLFELTLKGQKASIDKLRYAPDNKSAKSDKTKELAWLKIRWKSPQGKESQLVEFP
                     LAFAIKAPSEDMRFRAAVAAYGQKLRGSEYLNNTSWQQIKQWAQKAKGEDPQGYRAEF
                     IRLIGLAKDLDNSQN"
BASE COUNT         4286 a         4978 c         4688 g         4590 t
ORIGIN      
        1 ctttatagca aatataagtg gacaggtatt caatgacgga tggaaaaaaa atgtcaggat
       61 ttttgccttt ttcgcgacca gcaatgggcg tagaggaact cgctgcagtt aaagaggttc
      121 tcgaatccgg ttggatcaca accggtccga agaatcaggc gcttgaacaa gctttttgcc
      181 agttgacggg aaatcagcat gccatcgcgg tcagttcagc caccgccgga atgcatatca
      241 cgctaatggc gttggaaatt ggcaagggcg atgaagtgat tacgccttcc ctgacctggg
      301 tttcaaccct caatatgatt tccttgctgg gtgcaacgcc ggtaatggtg gatgtcgacc
      361 gcgatacgct gatggtcacg cctgaagcga tcgaagcagc cattacgcca cgcactaaag
      421 ccatcattcc ggtgcattat gccggtgcgc cagcagatat tgacgccatt cgcgccattg
      481 gcgaacgtta cggcatcgca gttatcgaag atgctgccca tgccgtcggt acgtattaca
      541 aagggcgaca tattggcgca aaaggtactg ctattttttc gtttcacgcc atcaaaaata
      601 ttacctgtgc ggaaggtggc ctgattgtta ctgataatga aaatcttgcc cgccagctgc
      661 ggatgctgaa atttcacggt ctgggtgtcg atgcctatga cagacatacc tggggccgtg
      721 caccgcaggc tgaagtctta acgccgggct ataagtacaa tctgaccgat attaacgccg
      781 cgattgccct gacacagtta gtcaaattag agcacctcaa cacccgtcgg cgcgaaattg
      841 cccagcaata tcagcaagca ctggcagctc tcccctttca gccattaagc cttcccgcct
      901 ggccgcacgt tcacgcctgg catctgttta ttattcgtgt cgatgaacaa cgttgtggta
      961 tcagtcgcga tgcgttgatg gaagcgttaa aagaaagagg cattggtacc gggttacatt
     1021 tccgcgccgc tcacacacaa aaatattatc gcgagcgttt tcccacgctg tcgttaccga
     1081 ataccgaatg gaatagtgaa cgcatctgtt cgttgccgct gttcccggat atgaccaccg
     1141 ccgatgccga ccgcgtcatc acagccctta agcaactcgc aggacaataa gccatgtttg
     1201 aaatccaccc tgttaagaaa gtctcggtgg ttattcccgt ttataacgag caggaaagct
     1261 taccggaatt aatcaggcgc accaccgcag cctgtgaatc actggggaaa gagtatgaaa
     1321 tcctgctgat tgatgacggc agtagcgata attccgcgca tatgctggtc gaagcctcac
     1381 aagcggaggg cagccatatt gtgtctatct tgcttaaccg caattacggg caacattcag
     1441 cgattatggc gggattcagt cacgtcaccg gcgacttaat tattaccctc gatgccgatc
     1501 tccagaatcc gccagaggaa atcccccgtc tggtggcaaa agccgatgaa ggttacgacg
     1561 tggtagggac tgtacgccag aaccgccagg acagctggtt tcgtaaaacc gcttcaaaga
     1621 tgattaaccg gcttattcag cgcaccacag gcaaagcgat gggtgactat ggttgtatgc
     1681 tgcgcgccta tcgccgtcat attgtcgatg cgatgttgca ctgccatgaa cgcagcacct
     1741 ttatcccgat tctggcgaat atcttcgccc gccgtgccat tgaaattcca gtgcaccatg
     1801 ccgagcgtga gtttggtgaa tccaaataca gctttatgcg cctgattaat ttgatgtacg
     1861 acctggtgac ctgtcttacc acaacgccgc tacgtatgct gagtctgctc ggcagcatta
     1921 ttgcgattgg cggttttagc attgcggtgc tgctggtgat tttacgcctg accttcggac
     1981 cccaatgggc ggcagaaggc gtctttatgc tatttgccgt gctgtttact tttattggcg
     2041 ctcagtttat cggcatggga ttgctcggtg aatatatcgg caggatctac accgatgtcc
     2101 gcgcccgccc ccgctatttt gttcagcaag ttatccgtcc atccagcaag gaaaatgaat
     2161 aatgaaaacc gtcgtttttg cctaccacga tatgggatgc ctcggtattg aagccctgct
     2221 gtctgccggt tacgaaatta gcgccatttt tacccatact gataaccccg gtgaaaaagc
     2281 cttttatggt tcggttgccc gactggcagc ggaaagaggc attccagttt atgcgccgga
     2341 taacgttaat catccgctgt gggtggaacg cattgcccaa ctatcaccag aggtgatttt
     2401 ctctttttat tatcgccatc ttatttgcga tgagattttg cagctcgctc cgagaggtgc
     2461 atttaatctg catggttcgc tgttaccaaa atatcgtggt cgcgcgccgc tgaactgggt
     2521 gctggtgaac ggtgaaacgg aaactggcgt tacattgcac cgaatggtga aacgtgccga
     2581 tgccggggcc attgtggcac aactgcgcgt tgccattgcg ccagacgata tcgccattac
     2641 gctgcatcat aaattgtgcc atgccgcgcg ccagctactg gaacagacat tacccgccat
     2701 taaacacggt aatattctgg aaatcgccca gcgcgaaaac gaagccacct gttttggtcg
     2761 cagaacgccg gatgacagtt tcctcgaatg gcataagccg gcatccgtac tgcacaacat
     2821 ggtgcgtgcc gttgccgatc cgtggccggg tgccttcagc tatgttggca atcagaaatt
     2881 caccgtctgg tcgtcgcgtg ttcatcctca tgccagcaaa gcacagccgg ggagcgtgat
     2941 ttctgttgca ccactgctga ttgcctgtgg cgatggcgcg ctggaaatcg tcactggaca
     3001 ggcgggcgac ggcattacta tgcagggctc gcaattagcg cagacgctgg gcctggtgca
     3061 aggttcacgc ttgaatagcc agcctgcctg tgccgcccga cgccgtaccc gggtactcat
     3121 cctcggggtg aatggcttta ttggcaacca tctgacagaa cgcctgctgc gcgaagatca
     3181 ttatgaagtt tacggtctgg atattggcag cgatgcgata agccgtttta tgaatcatcc
     3241 gcattttcac tttgtcgaag gcgatatcag tattcattcc gaatggattg agtatcacgt
     3301 caaaaaatgt gatgtcgtct tgcctttggt ggcgatagcc acgccgattg aatatacccg
     3361 caacccgctg cgcgtatttg aactcgattt cgaagagaat ctgcgcatta tccgctactg
     3421 cgtgaagtac cgtaagcgaa tcatcttccc gtcgacttca gaagtttatg ggatgtgtag
     3481 cgataaatac ttcgatgagg accattctaa tttaatcgtc ggcccggtga ataaaccacg
     3541 ctggatttat tcggtgtcta aacaattact tgatcgagtg atctgggcct atggcgaaaa
     3601 agaaggttta cagttcaccc tcttccgccc ctttaactgg atggggccac gactggataa
     3661 ccttaatgca gcgcgaattg gcagctcccg cgctattacg caactcattc tcaatctggt
     3721 agaaggttca ccgattaagc tgattgatgg cggaaaacaa aaacgctgct ttactgatat
     3781 tcgggatggt atcgaggcgt tataccgcat tatcgaaaat gcgggaaatc gctgcgatgg
     3841 cgaaattatc aacattggca atcctgagaa cgaagcgagc attgaagaac tgggtgagat
     3901 gctgctggcg agcttcgaaa aacatccgcc gcgccattac ttcccaccgt ttgcgggctt
     3961 tcgtgttgtc gaaagtagca gctactatgg caaaggatat caggacgtag agcatcgtaa
     4021 accgagcatc cgcaatgccc gccgctgcct ggactgggaa ccgaaaattg atatgcagga
     4081 aaccatcgac gaaacgctgg atttcttcct gcgcaccgtt gatcttacgg ataaaccatc
     4141 atgaccaaag tcggcttacg cattgatgtc gatacctttc gtggcacccg tgaaggcgtg
     4201 ccgcgtctgc tggaaatctt gagtaagcat aatattcagg ccagcatttt tttcagcgtc
     4261 ggcccggaca atatgggccg ccatctctgg cgattggtga agccacagtt tttgtggaag
     4321 atgctgcgct caaacgcggc atcgctttat ggctgggata ttttactggc aggtacggcc
     4381 tggccaggta aagagattgg tcatgccaat gctgatatca ttcgtgaagc ggctaaacat
     4441 cacgaagtcg gcctgcacgc ctgggatcac catgcctggc aagcccatag cggtaattgg
     4501 gatcggcaaa caatgatcga cgatattgca cgcggccttc gcaccctgga agagattatc
     4561 ggtcaaccgg taacctgttc tgccgctgct ggctggcgtg ctgaccaaca ggtgatcgaa
     4621 gcaaaagaag cgttccattt gcgctacaac agcgattgtc gtggggcgat gccgtttcgt
     4681 ccattgctcg aatcaggaac ccctggcact gcgcaaattc cggtgacctt acccacctgg
     4741 gatgaagtga ttggtcggga tgtgaaagca gaagatttta acggttggtt actcaaccgc
     4801 atccagcgag ataaaggcac gccggtttat accattcatg ccgaagttga aggctgcgct
     4861 tatcagcata attttgtgga tctcctgaaa cgcgcagctc aggaaggcgt gacattttgc
     4921 cctttaagcg aactgttatc agggacgttg ccgctcggac aagttgttcg cggaaatatt
     4981 gccggacgtg aaggctggct gggttgccaa caaattgcgg gtagtcactg atgaaatcgg
     5041 tacgttacct tatcggcctc ttcgcattta ttgcctgcta ttacctgtta ccgatcagca
     5101 cgcgtctgct ctggcaacca gatgaaacgc gttatgcgga aatcagtcgg gaaatgctgg
     5161 catccggcga ctggattgtt ccccatctgt taggactacg ttatttcgaa aaacccattg
     5221 ccggatactg gattaacagc attgggcaat ggctatttgg cgcgaataac tttggtgtgc
     5281 gggcaggcgt tatctttgcg accctgttaa ctgccgcgct ggtgacctgg tttactctgc
     5341 gcttatggcg cgataaacgt ctggctttac tcgccacagt aatttatctc tcattgttta
     5401 ttgtctatgc catcggcact tatgccgtgc tcgatccgtt tattgccttc tggctggtgg
     5461 cgggaatgtg cagcttctgg ctggcaatgc aggcacagac gtggaaaggc aaaagcgcag
     5521 gatttttact gctgggaatc acctgcggca tgggggtgat gaccaaaggt tttctcgccc
     5581 ttgccgtgcc ggtattaagc gtgctgccat gggtagcaac gcaaaaacgc tggaaagatc
     5641 tctttattta cggctggctg gcggttatca gttgcgtact gacggttctc ccctggggac
     5701 tggcgatagc gcagcgggag cctgacttct ggcactattt tttctgggtt gagcatattc
     5761 aacgctttgc actggatgat gcccaacata gagcgccgtt ctggtactac ttgccagtca
     5821 tcattgccgg tagcctgccg tggctgggat tactccccgg tgcactgtac gctggctgga
     5881 aaaaccgcaa gcattccgca accgtctatt tgttgagctg gacgataatg ccgctgctgt
     5941 ttttctccgt cgctaaaggc aaattgccca cctatattct ttcctgcttt gcacctctgg
     6001 caatgctgat ggcgcattac gcgttgctgg cagcaaaaaa taatcctctg gcgttgcgga
     6061 ttaatggctg gattaacatc gcttttggcg tcactggcat tattgccaca tttgtggtct
     6121 ccccctgggg accaatgaac acaccggtgt ggcaaacctt cgagagctat aaagtctttt
     6181 gtgcctggtc tattttttcg ctatgggcat ttttcggctg gtacacctta acaaacgtcg
     6241 aaaagacctg gccttttgcc gcgctttgcc cgctggggct ggcgttgctg gtaggatttt
     6301 caattcctga cagagtcatg gaaggaaaac atccgcaatt ttttgtcgag atgacacaag
     6361 aatcacttca gccaagccgc tatattctta ctgatagcgt cggtgttgcc gcaggtctgg
     6421 catggagcct gcaacgcgat gacatcatca tgtatcgcca gacaggtgag ttgaaatatg
     6481 gccttaatta tccggatgca aaagggagat ttgtcagcgg tgatgagttc gcaaactggc
     6541 ttaatcaaca tcgtcaggag gggattatta cactcgtgct ttcggttgac cgcgatgaag
     6601 atatcaatag tctcgccatt ccgcccgcag atgtcatcga tcgtcaggag cgtctggtgc
     6661 taattcagta tcggcccaaa tgatctggct aacattagtc tttgccagct tgcttagcgt
     6721 tgccgggcag ttgtgtcaga aacaggcaac ctgctttgcg gcggtaaaca aacggcgcaa
     6781 acatatcgtg ctgtggctgg gactggcgct ggcttgtctt ggccttgcca tggtgctctg
     6841 gctgctggtc ttgcagaacg taccggtagg cattgcctac ccgatgttaa gtctgaattt
     6901 tgtctgggta acgctggctg cagtaaaact gtggcacgag ccggtatcgc tgcgtcactg
     6961 gtgtgggttg gcgttcatta ttggcggcat tgtgatcctc gggagtacgg tgtaatgggc
     7021 ctgatgtggg gattattcag cgtcattatc gcctccgctg cgcaattaag cctgggtttt
     7081 gcggcgagtc atctgccgcc aatgacgcac ttatgggatt ttatcgcggc gctgttagct
     7141 tttggcctgg atgcccggat actgttactc gggctgctgg gttatctgct ttccgttttt
     7201 tgttggtata aaacactaca taaacttgcg ctaagcaaag cctatgcctt gctcagtatg
     7261 agttatgtgc tggtatggat tgcttcaatg gtacttcccg gctgggaagg gactttttcg
     7321 ctgaaagcac tactgggagt agcctgtatt atgagcgggt tgatgctgat tttcctgccc
     7381 acaacaaaac aacgttactg agttttccct gccgctttac actgccgttc ccactcatcg
     7441 gggctataac agcttgcaga aagaactttc accgtgtgca gcttttcacg attaatacag
     7501 taaagcgcat tctgcaaagg cgagagtaaa tcccccactt tcacggcgaa gtcactcttc
     7561 acttcggcga tcatttttat cgcgccgcca gcatcgcaga gcataattac atgtctgtta
     7621 tcttgtttat tgcagcacgt ttttttgacc agccattcca ttgcattatc ctgtttgcta
     7681 agagttttca catcaattta cagattaaca gataaactct ttatcaacgg caatggaatg
     7741 gcagaaatga gaagtgaaac ggagggttaa ttattgctgg cgttgcaccc actcttttag
     7801 cgcctgacgt gaaattttaa taccgccgtt tttcagctcc ggcggcagag tcagccagcg
     7861 caccggttgt tgaaaacgag ccagcttatc tttcacccat tcgtcaagat caacgctttg
     7921 ctggtcatac tccaccaccg ctaccggccg atgaccaaac tccttgtcgg caacgggaac
     7981 gataaacacc tgcaaaaccg caggatgtgc agcaataaca cgctcgactt cttccggctg
     8041 aataccctca ccgccactga agaacagatt atctaaacgt ccgacaatgg tcagcttgcc
     8101 attatgcatc tcaccgcgat cgcgcgtggc gtaccagcct tcgtcattaa ccagtggaac
     8161 ccgttgcccg ttacgccagt aaccttctgc catactggca gcccgcagcc acacttcatc
     8221 attaacgatt ttcacttccc gacccggcag cggcgaacca acgtctgcca gaccgtcggc
     8281 ttctttcgca cacactgtgg aggcaaactc ggtcagacca tagccgcaaa agcaccgaat
     8341 cccctgctcg cgcgcctgtt ccgtcaactc gaccgggata gccgcgccgc caagtaaaac
     8401 cgctttcagg gaaacggaac tacggttaac cagcaaacgc cagagttgtg ttggcaccag
     8461 tgaagcgtga gtacagcctg ccagcatctg ctccaatggc tgtttatcac gcaccgtcat
     8521 ccgcgcacca gcgtataacc agcgccacat aattccctga ccggagacgt gaaacagcgg
     8581 taaagagagc aaccagtcat cgtgatcgcc aaacggaatc agcgataaca ccccttcggc
     8641 gctgacaaga tgagcctgat aagtatgaac agcggctttc ggcaagccag tggaaccaga
     8701 ggttaacgtc attgagcaca gacgcgtcgg ctgccacgcc gcggcatgtg cgccttcaac
     8761 cagctgaatg tgcagcgacg ctaacgccgg aaacgtgttt tccccttccg gcaccagcgc
     8821 aaattgcagc gtcagattgg gcagcaattc ctcaagcagc ggttgcggca gctgagggtt
     8881 cacgggcaac acccgcgccc cgcattgcag taacgccagc caggccagca gcgtttgcgg
     8941 cgtattccac gcccgcaaca tcacgccgct gccctcaacc acaccctgca ccgcaaagct
     9001 ggaggctaat tcatcaacgc gagcacaaag ctcgcgccag ttgagttgct cgtcattgag
     9061 acgtaaggcg atggcttctc cccgcacttg ccgccagtga cgccacggcc agtcagagaa
     9121 gatcataaca gccgctccag tgcatcaacg tccactaacg gcagtgggct ccccggccag
     9181 cgacgaacct gctgcgcctg catcagatcc agcgtgtcca gtcccggaat ggtgtctggc
     9241 gttaaccagg cagcaatccg cgccagttgc gttagaccta agctcgactc aatggaagaa
     9301 ctgatcaccg ccgtcagccc cagcgcatgc gccgcctgca cttgctcgcg tactttatcc
     9361 aggctgcccg tgagcgtggg cttgataacc accgcgcgca cgccctcttc agctacaaag
     9421 gcaaaatctg gctcgcgcag gctttcatcc caggcaatgg caatgccggt ttcacgggca
     9481 aacgcacgcg aatcatcgcg ggttttgcac ggctcttcga gaaacgcgat gcggtggcga
     9541 taatccgggt taacgtattt ggcaaactgc tggcctttca gcggtatcca ggcgcgattc
     9601 gcgtcaagac gcaaatgcag atcaggaatt gcctccagca acagattcac caccatgccg
     9661 tcgcgcacgg cttcgtacaa tcccactttg acctttgcca ctttctcgcc aggcatatcg
     9721 gcaagtttga ggatcagatc gtcaggatcg ccattacaca gcggtgccgc acggtaattg
     9781 gccgcctgcg ggagcgtatc tgccagctcc gccaatgcac aacttacgcc aaaggcaacg
     9841 gaaggcatct gcggtaattc gcaatcgcct gccagccagt tatttaccca ggcaagcagc
     9901 acactttgcg catcttccca ggtttcctga ctgaagcccg gcagtgggga gatctccccc
     9961 cacccttcgc gctcgccctc acgcaggcaa acatacagcc cgtcgcgggt ttttaaccgc
    10021 ctgtcgcgca gaaccacccc cgcgtccatg gggatctgcc agcggtatac ctgcgcgcta
    10081 cgcattacgg attccgttta aatttgctga aatcaggctg acgtttctgg ttgaaagcgt
    10141 tgcgaccttc ctgaccttct tccgtcatat agaacagcat ggtggcgttg cccgccagct
    10201 cctgcagacc tgcctgaccg tcacagtcgg cattcagcgc agctttcagg cagcgcagtg
    10261 ccatcgggct gttttgcaac atttcacggc accagcgcac ggtctctttt tccagatccg
    10321 ccagcggtac aacggtgttc accaggccca tatccagcgc ctgttttgcg tcgtactgac
    10381 ggcacaggaa ccagatttca cgtgcttttt tctgcccaac aatgcgcgcc atataggaag
    10441 cgccccagcc gccgtcgaag gaaccgactt tcgggccagt ctgaccgaag atggcattat
    10501 cagccgcgat agtcaggtcg cacatcatgt gcaggacgtg accgccaccg atggagtagc
    10561 cagccaccat cgcgacaacc ggttttggac aggtacggat ctgacgctgg aagtccagca
    10621 cgttcaggtg atgtacgccg gaatcatctt tatagccgcc gtaatcacca cgtactttct
    10681 ggtcaccacc ggagcagaac gctttatcgc ctgcaccagt cagaatgatc acgccgatgt
    10741 tgtcgtcata acgcgcatcc gccagcgcct ggatcatctc ttttaccgtc agaggacgga
    10801 aggcattgcg cacctgcgga cgattaatgg tgatttttgc gataccgtcg gtggattttt
    10861 cataacgaat gtcctcaaaa ccttcggagc agtcgtgcca ttcaaccggt gcgtaaagca
    10921 ttgcttcatc aggataaatc atattgtgtc ctttagtcag aaacgcaaga tctgcgccag
    10981 acttgcgatt acgccagcgg gattttcccg gtgcgcgtta tgtccggcgc gaggaatgac
    11041 atggcagtcg gcagccagtt ccgccgccag agcgcggaat ttgctgtcac gttcaccaca
    11101 taaataataa aacgcaaatg tgcgggcgct aaggttagca cgtaaatcgg gctggacggc
    11161 gagagaagtc gcctccagca tggcggcaag ggttgcgcca ttattgttgc tgcgcagcgc
    11221 caccagctcc cggcgttgat cgtcattgag cgaggcaaaa acaggctgtt gataccaatc
    11281 ggcaaatacc gccgttaacg gttctgtacg aaaacgctgc gcccattggc gatcggaacg
    11341 ctgacgttcc gcgcgttgtt cagcattttg caatccagga tgcccgcctt cgacaacaac
    11401 tccacaaagc cccgccagct cctggcaagc cgccatcatc gccacccgtc cgccaagcga
    11461 gtaccccacc agccagaagt taaggatgtt gtaactaacc agcgttttac acaataagcc
    11521 ggtgacatca tcaaatccat cgacgctgat cgtcgctgaa ccaccgtgac ctgggagatc
    11581 aacatacaac cgtgagtagt cggcaaacac ctcgcccact tcttgccatt catggcaatc
    11641 gccggaaaaa ccgtgcaaaa acaccagcca gggtaaacct ggtttaccgt gttttgcctg
    11701 cgcgtgcagg atcataaatg gcttacctgc gccagcagtt gctggagggt ttgcgcacca
    11761 tcggtgtcgt taaccaccat ttcaattacc gtggtggtcg gcgtgcgcca ggcgtcggca
    11821 agtgccgttt caagttcctg ccagttttgc ggacgatgat atttcagctc gaacatcgcg
    11881 gcggcgtgct caaaatggac gttttgcggc atcagataga aacgctcgcg ctcgtttttc
    11941 ggcgttggca acagcgagaa aatttgcccg ccgttgttgt tcaccacaat taataccagc
    12001 ggcgcggaaa cctgacgtaa taacgccagc gcgttgagat cgtaaagtgc ggagagatcg
    12061 cccacaatcg ccagcgtcgg tttgccgctt gcccgctgaa cgccggcggc agtcgagagc
    12121 agcccgtcga taccgctggc accacggttg ctgtacaccg ggtaacctgc cggaagttgc
    12181 gaaagcgcat caatcagacg caccaccagg ctgttaccga caaacaattg cccctgttca
    12241 ggcaagtagt cgctgatgcg atgcgccagt tgcgcttcgc caaacgcatc gcgacgggca
    12301 ataaccgcct gcattgcctg ttccgccagg cgcgggattt caacgcacca gggctggcgt
    12361 ttttctgccg gatgcagctc cagccagtcg gcaatattgg caattaagcg acgtccgcga
    12421 tggtgtgccg gatcaagtcg cccttcaatg tcatcaacaa tccagtactc ttctggttca
    12481 cagcttgcct gccattggag gagtcgtttc cccgtcaggc tgcttcccag ttgcaccact
    12541 atttgtgcct gttgcagttc gctggtcgct ttggcattgc ctaaccagag atcggcacac
    12601 ggcagcggct gtccggtttg cgacagcacg tcgccaatca gcggccagcc aagagtttgc
    12661 gcccacagtg caactttttt gccctcttcc gcactcatgc gcccggcaac caccacgccg
    12721 cgcttttgtc gccagaagaa ccagtcacgc tgtttttcac tttcccgacg aggcgcttca
    12781 cgcagccacg gtttgtcgtc ctgccaccag tcgcccagcc gctgttgcca gctaatcccg
    12841 gtgtcgtcca tttcgccata cagcggttca gcaaacgggc agttgatatg gactccacca
    12901 gcatgaagcg taccgagagc gtggtcgatg gtagaaacca gccaacgtgc ggggatatcc
    12961 tgggtcgggc gcggcagtga aatactgtgc gtggggtgag aggcgaacat tcccggctga
    13021 cgaatcgcct gattcgcgcc gcagtcaatc agctccggcg ggcgatcggc ggttaacagg
    13081 atcagttttt ctccggttaa cccagcttca atcagtgccg gatagagatt tgctaccgcc
    13141 gtgccggagg tcacaatcac cgccaccggc tgcttgctga ctttcgccag ccccagtgcc
    13201 agatgcccca gtccacgctc atcgaaatgg gtatgatgaa tgaatgcgga attctccgcc
    13261 gccgctaacg ttaacggtgt agaacgcgag ccaggggcga tacagatgtg tctgacgccg
    13321 tgacgcgtta atgcttccag aatgaccgcc gcccagcgtc ggttaaatgc gcttactgac
    13381 atgagtttgt ccggtatcaa tattgcggct aagtataagg agctacaaaa atagattatt
    13441 gatatgaatc ggtaatgatg cgactcatta ctattccatt tgtaataaag tacgcagccc
    13501 tgccgctttg ttgtcgattt cctgccactc ttgctcgggg tcggaaccac ggacaatgcc
    13561 tgcgccagca tatagccgca cgatattgcc gctgattttt gctgagcgca gggaaacgca
    13621 gaattcactt tgctgtaatg agagatagcc cgcagaaccg gcgtaccatt ctcgagtgaa
    13681 cggttcgtga cgggcgataa actgtcgcgc cagatcgcgc ggtaagccag caactgctgc
    13741 cgttggctgc aactgatgta aacagatcac atcatccgct ttgttgagtg aagtccagat
    13801 acagcggcga agatgctgta ctttacgtag acgcagtacc tgcggcggta aaacatccag
    13861 cgtttgggta tcggcctgca atcgttggca gatatcttcc accaccaaca tgttctcgcg
    13921 ctggttttta tcatccgcca tcagccactc acctaactgc tgcgcctgct tatcatcagg
    13981 atgatttgct accgttcccg ccagcgcttc ggtacgcagc gctttgtcac gccgccgcca
    14041 taaccgttcc ggtgaagaac caagaaaagc attttcgcca tcaaaggcca tgtaaaaatg
    14101 gtagcaattc agattgagcc gacgactagc agccatcatc gctgccgcat tgaccggact
    14161 tgcgaaatgc aggtcagttg cccgagcaag cacaactttg tcgagctcgc cttcggcgat
    14221 ggttttcgtt gccagctcga ttaattgcgt ccagcccgtt ttgtccggcc agtgttgttc
    14281 tcgcgtggtg gttaaatgta atccaggcag gggcttgata ctcaccagtg tggcgataaa
    14341 ttcttttgcc ttgattgcat cgtgctgaag ggagctttcg ctgaatagcg tcagccgcag
    14401 cgtggcttta ccgccacaac gtcgccattc caggcgtggt aaaagtaaat tgccctgcga
    14461 cgggtcaaac gcattcaacc cccagatgcg taagtcggcg tgttccgggt gctggcgaag
    14521 aaaacgttgt gcctggtcca acgacgtaaa acgggtaatc gcacccagga cggcagcttc
    14581 ttcatcacca ttacgttgtt gccagtagaa ttgcggatac acctgctgac tggccagcca
    14641 gctcaaggca tcaaaagcgt ctttgagagg gaaaggaata tcgataaccc gaatgccggg
    14701 tgtcgccgga atctcttgcg acaaatggcg cagtagattt tccagcgccg tagtaagtga
    14761 ttgcacgcgg acctctccct gttaaaagcc tcacattata cggggtacga caaaaaaaat
    14821 gcagtacccc ggtgtaggga ggttttaacg gcgtgccagc aacagtccta gaaccagccc
    14881 aacggccgca cccacaccaa ttccttgcca gggtttttcg tggacgtagt catcagcacg
    14941 ataaacagcc tgcttcgccc gatagtaata actgtctgaa gcctgactaa cccgtttttt
    15001 cacatcatcc agcgcttttt ctgcacgcgc tttcagctca acatatttct gatcggcggg
    15061 atcgccagag gagcggagca cctcttccag tgtttcacta agcagcgtca ggtcgtcatc
    15121 aatacgtgta tcaccaaact gattagacat tctcgttctc catttgtgta aaacctgtca
    15181 ctaactatag acaagggtta ttgattacgc ctgaattacc tcgcgtgcca tcccaatgtg
    15241 tgggatacca tcttcttcat aaatatcggt cacagggatg aaaccaaatt gcccataaaa
    15301 gttttgtaaa tgcgcctgcg cccccagata aatcggcttc tccggccagc ggcgagtaca
    15361 cgattccagt gccttactca tcaattgctg accgattttt tcaccgcgta aggcttcgct
    15421 gacaatcacc cgaccaatca caacgggttg cagttcgtcg tcacttttca gaatcctcgc
    15481 atacgccacc agtgtcccgt tatgccaacc gagaatgtgg cgattttctc cctccagatc
    15541 gtcaccatcg atatcctggt aaggacagtt ttgttccact acaaataccg cacaacgcag
    15601 ttgtaataag gcataaagct gagaaacgga taaatcagaa tgatgcagat cttgccagtc
    15661 gatcatggaa ggctcctttg tgctgtttgc tttatactag ccggaatatt ttcagcagcg
    15721 tgatgaactc atggaattaa tttttttagg tacttcagcc ggtgtgccaa cccgtacgcg
    15781 taatgtcacg gcaatattgc ttaacctgca acatccgacc cagagcggac tttggttatt
    15841 tgactgcggt gaaggcaccc agcatcagct actgcatacc gcctttaacc ctggaaaact
    15901 ggacaagatt tttatcagtc accttcatgg cgatcatctc tttggtttac ccggcttgct
    15961 gtgcagtcgt tctatgtcag gcattatcca acccttaacg atttatggtc cgcacggtat
    16021 ccgtgaattt gtggagaccg cgctgcggat tagcggctca tggaccgatt atccgctgga
    16081 aattgtcgaa attggcgctg gcgaaatttt cgatgatggc ctgcgcaaag tgaccgctta
    16141 tccgatggaa cacccactgg aatgttatgg ttatcgtatt gaagaacatg ataaactagg
    16201 tgcattaaat gcccaggcat taaaagctgc tggcgtgccg cctggcccgc tgtttcagga
    16261 attaaaagcg ggcaaaacaa tcatgctgga tgatggaagg cagattaatg gcgcagatta
    16321 cttagccgtt ccagtgccgg gtaaagcgct cgctattttc ggcgataccg gcccctgcga
    16381 tgccgcgctg gagctggcta aaggcgtcga cgtaatggtt cacgaagcga ctctggatat
    16441 ggcgatggag gccaaagcca acagtcgcgg ccatagttct acacgccagg cggcggcact
    16501 agcccgtgag gctggagtcg gtaagctaat cattacccac gtcagctcgc gctatgatga
    16561 caaaggttgt cagcacctgt tacgtgaatg tcggtcaatt ttcccggcga ctgaactggc
    16621 gaatgatttc gcggtgttta gcatttaagt cgaaagcggt ggttagttag cagattaatt
    16681 taacgatacc aacgcattta tttgacattt tttcatctgt ttgccggata cgtttgatgt
    16741 cgatatcagg caaacagaat tatcattttt agttttgtga attgtccaga tctttcgcca
    16801 gtccaatcag gcgaataaat tccgctctgt aaccctgtgg atcttcccct tttgccttct
    16861 gagcccactg tttgatctgc tgccaggagg tattattcag gtattcagaa ccgcgtaact
    16921 tttgcccata tgcagccact gctgcgcgaa aacgcatatc ttctgaaggt gcctttattg
    16981 cgaatgccaa cgggaattca actaactgac tttcttttcc ctgtggggat ttccaacgaa
    17041 tctttaacca ggcaagttct tttgttttgt ccgatttcgc tgatttgtta tccggggcat
    17101 agcgtaactt atcaattgat gctttttgcc ctttcagcgt taattcaaat aacaatgtta
    17161 tatgtttacc tgcgccaata tcacccgcat caacgttgtc gttattaaaa tcctctgccc
    17221 taagttggcg cttttcataa cctatctgac ggtattccgt tacccacgcg gggttaaact
    17281 caatttgcgc tttgacatct tttgctacgg taatcaacgt ctgccgcatt tcactgttca
    17341 acactttctg cgcctctgag agggtatcaa tgtagctata gttgccgtta ccaacatcgg
    17401 caattcttac catcattgcc tcgttataat tgctatcccc cacgccaagc gtcgacagag
    17461 taacaccgga ctcacgctgt tttttgacca ttgattcaat cgattttgga tcgtcaatgc
    17521 caacgttaaa gtcaccgtca gtggccaata aaatgcgatt gataccacct ttaataaacc
    17581 ccttcgctgc ctgctgataa gccatctcca gcccggcacc gccattggta ctgccttctg
    17641 catccagcga atcaattgca gcattaattt ccgccttgtg actaccggag atagaaggca
    17701 acgcaatacg agagtcgcca gcgtaggtca cgatggcaat gttatcctgc tcacgaagtt
    17761 ctttgaccaa taatttcaac gaagactgga taagtggcaa acgttcatca gaaatcattg
    17821 aaccagaagt gtcgataaga aagaccagat tagaagctgg taactcttta cttttgcgat
    17881 ctttcgccag gatatcaact ttcagcaatg ttcgctgttc attccagggt gcaggtgcca
    17941 attcgtagcg catagcgaaa ggtattggct tagaggccgg aatagattgt ttgtctttga
    18001 tatcccagtc agacgggaaa taattgacta cctcctccac ccgcacagcg tctggcggag
    18061 gtaacagccc ttgattaagg aaacgccgca cattcgcata actgccggtg tcgacgtcaa
    18121 ggctaaacgt cacgagtggg ttttgcgcta cctgcttaac cggattatca tcgaactgct
    18181 ggtagcgagc ggttcctgga tttgcgatat gtgtagcgtt tgctttagcc gctcgtgcaa
    18241 atgttggcgc ttcctgcaat ctcccctgca aagtctgttt gtctgaatat tgttgcactt
    18301 cttgctgggc taaggctttc gcgtctgcag cagctttagc ggcggcggcg ctttgctcta
    18361 cctcttttat tgcagcttgt tgcgcgacaa gcacttgttg gtctgagggg gtactgggtt
    18421 gttgttgctg actttcctta tcctcaggtt ccggcccaca tcctgacaaa atcaaactac
    18481 ttataagtaa catgattata tttttatttc gcatataaaa tccttttaaa taaactcagg
    18541 gc
//
LOCUS       DAZGJT010000095        18101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZGJT010000095 DAZGJT010000000
VERSION     DAZGJT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.095"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(215..961)
                     /gene="cutC"
                     /locus_tag="ACHKFK_003639"
     CDS             complement(215..961)
                     /gene="cutC"
                     /locus_tag="ACHKFK_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO9053890.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     FVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLNNLAELGITRVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIECHQAK"
     gene            complement(975..1541)
                     /gene="yecM"
                     /locus_tag="ACHKFK_003640"
     CDS             complement(975..1541)
                     /gene="yecM"
                     /locus_tag="ACHKFK_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO9053891.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1757..3490
                     /gene="argS"
                     /locus_tag="ACHKFK_003641"
     CDS             1757..3490
                     /gene="argS"
                     /locus_tag="ACHKFK_003641"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO9053892.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3543..3935)
                     /gene="flhe"
                     /locus_tag="ACHKFK_003642"
     CDS             complement(3543..3935)
                     /gene="flhe"
                     /locus_tag="ACHKFK_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO9053893.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGVTLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIPAAEPL
                     RFIWEVPGGGRLIPPLKIQRNEVIVNYR"
     gene            complement(3935..6013)
                     /gene="flhA"
                     /locus_tag="ACHKFK_003643"
     CDS             complement(3935..6013)
                     /gene="flhA"
                     /locus_tag="ACHKFK_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO9053894.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEAKPVNMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6006..7154)
                     /gene="flhB"
                     /locus_tag="ACHKFK_003644"
     CDS             complement(6006..7154)
                     /gene="flhB"
                     /locus_tag="ACHKFK_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO9053895.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     AIISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(7363..8007)
                     /gene="cheZ"
                     /locus_tag="ACHKFK_003645"
     CDS             complement(7363..8007)
                     /gene="cheZ"
                     /locus_tag="ACHKFK_003645"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO9053896.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSIAGVVASQDQVDDLLDSLGF"
     gene            complement(8018..8407)
                     /gene="cheY"
                     /locus_tag="ACHKFK_003646"
     CDS             complement(8018..8407)
                     /gene="cheY"
                     /locus_tag="ACHKFK_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO9053897.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(8422..9471)
                     /gene="cheB"
                     /locus_tag="ACHKFK_003647"
     CDS             complement(8422..9471)
                     /gene="cheB"
                     /locus_tag="ACHKFK_003647"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO9053898.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSVPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(9474..10334)
                     /gene="cheR"
                     /locus_tag="ACHKFK_003648"
     CDS             complement(9474..10334)
                     /gene="cheR"
                     /locus_tag="ACHKFK_003648"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO9053899.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLAETLSTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10449..10579)
                     /locus_tag="ACHKFK_003649"
                     /pseudo
     CDS             complement(10449..10579)
                     /locus_tag="ACHKFK_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
     gene            complement(10625..12286)
                     /gene="tar"
                     /locus_tag="ACHKFK_003650"
     CDS             complement(10625..12286)
                     /gene="tar"
                     /locus_tag="ACHKFK_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO9053900.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(12431..12934)
                     /gene="cheW"
                     /locus_tag="ACHKFK_003651"
     CDS             complement(12431..12934)
                     /gene="cheW"
                     /locus_tag="ACHKFK_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO9053901.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(12955..14919)
                     /gene="cheA"
                     /locus_tag="ACHKFK_003652"
     CDS             complement(12955..14919)
                     /gene="cheA"
                     /locus_tag="ACHKFK_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO9053902.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQETDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPDDIAEDDITAVLCFVIEA
                     DQITFETVDVSPKISTPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(14924..15850)
                     /gene="motB"
                     /locus_tag="ACHKFK_003653"
     CDS             complement(14924..15850)
                     /gene="motB"
                     /locus_tag="ACHKFK_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO9053903.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIALVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLNGGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKHAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(15847..16734)
                     /gene="motA"
                     /locus_tag="ACHKFK_003654"
     CDS             complement(15847..16734)
                     /gene="motA"
                     /locus_tag="ACHKFK_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO9053904.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLEFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(16861..17439)
                     /gene="flhC"
                     /locus_tag="ACHKFK_003655"
     CDS             complement(16861..17439)
                     /gene="flhC"
                     /locus_tag="ACHKFK_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO9053905.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(17442..17792)
                     /gene="flhD"
                     /locus_tag="ACHKFK_003656"
     CDS             complement(17442..17792)
                     /gene="flhD"
                     /locus_tag="ACHKFK_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO9053906.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4341 a         5025 c         4433 g         4302 t
ORIGIN      
        1 tatatttgtc agttttggca atttaaatca cataatgaat gtgaatatat aaaatatttt
       61 tagcacaacg ctatgcgcac tgtcacccgc tcaacagatc acggcaatcg ttcgtttttt
      121 atactgctca gggataaaaa aaggcccctg ttgaaattgc aggggcctgg tacgagcaag
      181 catcatattg ggcgacatga tgcaacggta aaaatcattt ggcctgatgg cattcaatga
      241 ttcctttcat ttcagcaacc gccgccccgt ctacgacata acgcgaatac tcgtccgcgt
      301 gtgcatctga tgacatggac aatccttgat tacgataacg catcggtgag gcttgccacg
      361 ctcccgcgga gctatggact tccagcactc cggcatcgag gaagtggtgc aggttttccg
      421 cacggactcc tgctccggcc ataatgattg gagcatcacg atgggcaata agttccataa
      481 tttttgataa accttgcagt gcgtctgatt tttgccctga tgtcagtacc cgggtaatgc
      541 ccaattccgc aagattattg agtgtattta aagggttagc gcacatatcg aaggcgcgat
      601 gaaaagtcac tgccagcgga ccggcagcag ccattatttt ttccattcgt ggcatatcga
      661 cattcccgtc aacatcgaga acgcccgtca ccagtccagg aaaacctaat tcgcggactg
      721 tgcgcacatc ttcaagaatg gcggcaaact caccgtcgct gtagcaaaaa tcaccaccgc
      781 gtgggcggat aatcggatgc actgggatcg tcacccgctg gcgcacgaat ttcagtaccc
      841 ccaacgacgg cgttaagccc ccctcttttg gagctgcgca taattcaact ctgtcagcgc
      901 cgttttgctg cgccgttagc gcgcattcca tgctgtaaca gcaaatttcc agtaatgcca
      961 tttttgctcc ttagttacgc cgactgctcg ctggcaacga tctcttcaat ggaccacgga
     1021 tgaaacttaa tggttgtttt accatcggtg accgccagcg tcggattcgg taatcgttca
     1081 tgctcacctt ttggcgaact ggttttaact gagatccccg gcaggctgag tccttcatca
     1141 gaaagcaatg ccagcgcacg ggcattcagc gtttctggat cccccggcag aacgatttca
     1201 atatgttccc aaccttcgtg cgggtaacgt ttttccccgg gccacggtag ctccacaatg
     1261 gaaaactgcc agtgcgcaac ctgtaccggt tcatgcaatt taaacagaca aatcggtctg
     1321 ccattgatca tattttctga caaaagttcg ccgcactgtt caaacccgcg acgccagcgt
     1381 tcagcggtga cgttttgatg gcaacgcaaa gaaatgtgat cggcagtcag cggagtgata
     1441 tccaacccca gacggcggga aagttcatct aatgcgtggg taaatcgcgg taaatccgat
     1501 gcaatatcct gcagctcgtc gatagattgc cagttcgcca taatcactct tcgtctttca
     1561 ttaaaagcgc taatttaccc tgttgccctg cgccaaccaa ccgctgattt cacgccgctt
     1621 ctgatgcaat agtgaaaacg gcaatacgcc acgcgcacgt tgctgacgaa aacagccatt
     1681 tgcagtatac tcccgcccta atttctttaa ctggtgcggg caatttttgc tcgcttcatc
     1741 aatgtaaggt attccggtga atattcaggc tcttctctca gaaaaagtcc gtcaggccat
     1801 gattgcggca ggcgcgcctg cggattgcga accgcaggtt cgtcagtcag caaaagttca
     1861 gttcggcgac tatcaggcta acggcatgat ggcagttgct aaaaaactgg gtatggcacc
     1921 gcgacaatta gcagagcagg tgctgactca tctggatctt aacggtatcg ccagcaaagt
     1981 tgagatcgcc ggtccaggct ttatcaacat tttccttgat ccggcattcc tggctgaaca
     2041 tgttcagcag gcgctggcgt ccgatcgtct cggtgttgct acgccagaaa aacagaccat
     2101 tgtggttgac tactctgcgc caaacgtggc gaaagagatg catgtcggtc acctgcgctc
     2161 taccattatt ggtgacgcag cagtgcgtac tctggagttc ctcggtcaca aagtgattcg
     2221 cgcaaaccac gtcggcgact ggggcactca gttcggtatg ctgattgcat ggctggaaaa
     2281 gcagcagcag gaaaacgccg gtgaaatgga gctggctgac cttgaaggtt tctaccgcga
     2341 tgcgaaaaag cattacgacg aggatgaaga gtttgccgag cgtgcacgta actacgtggt
     2401 aaaactgcaa agcggtgacg aatatttccg cgagatgtgg cgcaaactgg tcgacatcac
     2461 catgacgcag aaccagatca cctacgatcg tctgaacgtg acgttgaccc gcgatgacgt
     2521 catgggtgaa agtctctaca acccaatgct gccgggtatc gtggcggacc tgaaagccaa
     2581 aggtctggca gtagaaagtg aaggcgcgac cgttgtattc cttgatgagt tcaaaaacaa
     2641 ggaaggcgaa ccgatgggcg ttatcattca gaagaaagat ggcggctatc tctacaccac
     2701 cactgatatc gcctgtgcga aatatcgtta tgaaacactg catgccgatc gcgtgctgta
     2761 ttacatcgat tcccgtcagc atcaacacct gatgcaggca tgggcgatcg tccgtaaagc
     2821 gggctatgta ccggaatccg taccgctgga acaccacatg ttcggcatga tgctgggcaa
     2881 agatggtaaa ccgttcaaaa cccgcgctgg cggtacggtg aaactggccg atctgctgga
     2941 tgaagcccta gagcgtgcac gtcgtctggt ggcagaaaag aacccggata tgccagccga
     3001 cgagctggaa aaactggcta acgcggttgg tattggtgcg gtgaaatatg cggatctctc
     3061 caaaaaccgc accacggact acatcttcga ctgggacaac atgctggcgt ttgagggtaa
     3121 taccgcgcca tacatgcagt atgcttacac gcgtgtattg tccgtgttcc gtaaagcaga
     3181 aattaacgaa gagcaactgg ctgcagctcc ggtaatcatc cgtgaagatc gtgaagcgca
     3241 actggcagct cgcctgctgc agtttgaaga aaccctcact gtggttgccc gtgaaggcac
     3301 gccgcatgtg atgtgtgctt acctgtacga tctagccggt ctgttctctg gcttctacga
     3361 gcactgcccg atcctcagcg cagaaaacga agaagtgcgt aacagccgtc tgaaactggc
     3421 acaactgacg gcgaagacgc tgaagctggg tctggatacg ctgggtattg agacagtaga
     3481 gcgtatgtaa tcgcgttata cggcaacagc cgatgtcatt gcagacatcg gcttttttat
     3541 cattaacgat aatttacaat cacttcattc cgctgaatct ttagcggcgg aattaaccgc
     3601 ccaccgccag gcacttccca gataaagcgc aacggttctg ccgccggtat accggaaaag
     3661 gccacggtgg ttccactctg cccctctaat tcgacacaac gagattgtga acacaagcga
     3721 acccgcagtc ctgcaggtgt cggaccgata agctgataac gccacgccac cagcgtcatc
     3781 aatcctgaag caggttgtcg cgtagaaaga ggcgctgacg acatcgattc accgcgatga
     3841 tttagcgtaa cgcccacgct actcgcctgc cacatcccct ccccggcggc ttgcaccagc
     3901 agcggaaaca ataatattgc taataaggct ctcattattt gccgccaatc gtcgctgtca
     3961 tacggatatg tcggttatct gatagttcca gattcgacag caccactaac tgcggcaagc
     4021 tgcggcgcag gaagcgagac aataatggtc gcagcgcgtg gttcaccaac agtaccggcg
     4081 gcgcacccaa catctcctga cgggatagcg cttcctgagt ttgcgccaac aagcgatccg
     4141 ccagccctgg ctccagtccc cccccgccct gcaatgcctg tagcaacaaa cgttccagcg
     4201 gtgtatcgag gccaataaca tggacttcat ctttgccagg aaaccactgc tgggtaatcg
     4261 cacgtcccaa cgccacgcgc acgacagcgg ttaattcatg cggatcgctc tggatgggcg
     4321 catgttccgc cagcgtttcg agaatggtgc gcatatcgcg aatcggcact ttttcatcga
     4381 gcagattttg cagcacttta tgcagtgtgg taagcgtgac gacgccagga acgagatctt
     4441 ccgtcagctt tggcatctcc tgggcgacgc gatccagcag ctgttgcgcc tcctgacgac
     4501 caaacagctc tgcggcatgc tggctaatga ggtggttaag atgcgttgcc accaccgtgc
     4561 tggcctcaac cacggtgtac ccctgaatct gcgcctgctc tttaagcgca ctttcaatcc
     4621 agatagcatt caggccaaat gccggatcga cggtcgcctc accaggtaac gtcccggcag
     4681 cggttccagg gttaatcgcc aaccaacgcc ccggataagc atcaccactg cctatctcca
     4741 cgcctttcat caaaatgcga tagcgggcag gttgcagatc catattgtcg cgaatgtgta
     4801 ccactggcgg cagaaacccc atctcctggg caaatttctt gcggatactg cgtatacggc
     4861 ccaacaactc accatcctgc tggaaatcga ccatcgggat cagtcgataa cccacttcca
     4921 ttcccagaga atcttccagt tgtacatcgt tccacgtcgc ttcgacaacg gtattattct
     4981 ctgccatgtt taccggtttt gcttcggcag gcgctttttg ttcacgtccg cggatccacc
     5041 acgccagccc cagcaatccg gcagtgaaca gcaaaaatac caggttcggc attccaggca
     5101 ccaggccgag taaaccgagc acggcggcgc ttaataacat aacgctcgga ttactgaaaa
     5161 gctgattcac catctgctcg ccaacatcct gatcggtgct gacacgtgta acgatgacgc
     5221 ccgcggcggt agaaatcacc agtgccggga tttgtgccac cagaccgtcg ccaatggtca
     5281 atagcgtata actttccgcc gcgtgcccca tgctcatgcc atgttgcagc acaccgacca
     5341 gcaatccgcc gacaacgtta atgaccatga tgaggatccc ggcgatggca tcgccgcgaa
     5401 caaacttact tgccccgtcc atcgagccgt aaaaatcggc ttcctgagtc acttcggagc
     5461 ggcgtttttt cgcttcatct tcaccaatca atccggcgtt aaggtcggcg tcaatcgcca
     5521 tctgcttacc cggcatacca tcgagaacaa agcgcgcgcc cacttccgca atacgcccgg
     5581 cacctttggt gataaccata aagttgatga tcacgagaat gacgaacacc acgataccga
     5641 tagcgaaatt gccaccaacg aggaagtgac cgaacgcttc gaccaccttt ccagccgccg
     5701 ccgcgccggt atgcccttcc atcaaaatga tacgggttga agccacgtta agtgccagac
     5761 gcaacagcgt ggtaaacaac aggatggtcg gaaacgcagc aaactcgagc gtgcgctggg
     5821 taaacatcgc caccagcaac accatgatcg acaaggcaat attgaaggta aacagcaggt
     5881 cgagtatgaa tgcgggcagt ggcagtacca tcatcgacaa gatcaacagg atcaaaatcg
     5941 gtccggcaag gatctgccat tgtgtcgatt tcaggtttgc aggcaggcgc agcatcgcgg
     6001 ccagattact catgggtcgg tttctcgtta ataaaatcca gggcttccgg caccggaaga
     6061 tgagtaggtt gtacagggcg ctgtccaccg gccagacgcc agcgtttcag ttgccagacc
     6121 caggccagca cttccgccac cgcggcgtac agttgacccg ggatttgttg accgatctcc
     6181 gcatgtcgat acagcgcacg cgccagcggc ggcgcttcaa gcgtcgggac gttattttca
     6241 gcgccaattt cacgaatgcg cagcgcgacc aaccctgcac ctttagcgac cactttcggt
     6301 gcgctcattt tgttttcgtc atactgcaac gctaccgaat agtgggtcgg gttattgaca
     6361 atgacatccg ctttcggcac atcggccatc atccgacgcc gtgcggccgc tcgctgcatc
     6421 tgacggatcc gccctttaac atgggggtcg ccttcgcttt gtttgaactc atcacgaata
     6481 tcctgccgtg acattcgcag ctttttcagg tggctgaaga tttggaaaaa gacgtcaaat
     6541 cccaccattg gaatgacacc aagcaccacc agcagcgcgc atagccctac cagatccatc
     6601 gcattaccca tggcggtaat cggagactcg gccattaagc gcatcatctg cggccagtga
     6661 tgccagagaa aaaaccccgt cacgctgcca accaggatgg ttttcaaaat tgccttaagc
     6721 aactccgcgc cagtctgtgc cgagaacatc cgtttaatgc ccggtagcgg gttgagtttg
     6781 gaaaacttcg gctgcaagga ttttccgcta aataccagcc cccccagcat aaccggagaa
     6841 ataatcgcca ccagcaccac gccgctaatc agcggtagca gcgccagcat ggcttctctg
     6901 atcagcagaa taatctgccc gaggatcaga ttcgggtcat tgataatact gtgatcaaaa
     6961 tgcagccctg cggagagcat gcccgacaat cgacgggcca gcgacacacc gccaaaccag
     7021 ataacactaa cgcccaccag caaaatcagc agtgaagtca gttcgcggga acgcgggatt
     7081 tgcccctctt cccgcgcttt ttctagtcgg tgaggtgtgg gggcttctgt tttgtcgtcg
     7141 ctctcgtcag acacgtcgcc aatcctggaa agagtattaa gcgtaaatga tgccagagcg
     7201 caaagtgttc aatggtttga gtaaggggca aaacaggcgg gatttagggc ttttgccaca
     7261 gcacatcagg catggtgaca tgtttgtcgg gtacggcgta aacgccttat ccaaccttga
     7321 agatctgatg tgacgcggcc acgtcacatc aggcaataca aatcaaaatc caagactatc
     7381 caacaaatcg tccacctgat cctgactggc taccacaccg gctatgctgg tatcgacctg
     7441 aggtccatta agcaaactct ggttttcacg ttttggacgc gactcctgtt ccgggatgtt
     7501 ttccaacagc accatcagca actgacgttc gatctcctga atgacatcca tcatccgctt
     7561 aatgacctgt ccggtgagat cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc
     7621 gttagtaaag ctggtatgtg cggggacatc tgccagaaat tgccgcgtgt ctgttaccag
     7681 ttcacgggcg tcggcaaggt caatcggatc ggcaaaccag tcatcccaac gttgggttaa
     7741 cgcttttgct gatttctcca tttgatcctg atgcggttgt gacgcctcaa cactgttcag
     7801 cgcccgctcc gcagcctggg cggtcatctg cacaacatag tacaaacgat cgcgcgcatc
     7861 ggggatagct tccgctgctt cggcaatggc ctgatccagc cccagttccc gcaaactgtc
     7921 gcgcagcata cgcgtcaggc tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc
     7981 agcaggtttg attgatggtt gcatcatagt cgcatcctca catgcccagt ttctcaaaga
     8041 ttttgttgag tttttcctcc agcgtcgcgg cggtaaatgg tttcaccaca tagccactgg
     8101 cccccgcttg cgccgcagca atgatgttct ctttcttcgc ttctgcagtc accattaaca
     8161 ctggcaatgc cgacatcgcg ccatccgcac gaatcgtttt cagcaactcc aggccatcca
     8221 tattgggcat gttccagtcg gagataacaa atccataacc gcctgcctgc aacttattga
     8281 gagcgtcgac gccatcttcc gcttcctcaa cattattgaa tcccagctct ttcagcaggt
     8341 tacgcactat gcgtcgcatg gtggaaaagt catccacaac caaaaattta agttctttat
     8401 ccgccatttc acactcctga tttaaatacg tatcgcctgt ccggcactaa tttttgccag
     8461 catttgctga cttacctggc taagatcgac cacttcgcag acaccaccca tattgatggc
     8521 ctcgcgcggc atgccgaaca ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc
     8581 cgcctgacgc atcgccaaca ttcccgccgc gccgtcgttg cccataccgg tcaggatcac
     8641 cccaaccgca ttacgccccg cctgtttggc cacagaatgg aacaacacat ctaccgatgg
     8701 ccgatggcga ttaaccgccg ggccatcgtg aattttgatt tgataatttg cgccactacg
     8761 cgctagctcc atatgccgat cgcccggcgc gatataggca tgtcccggca agacgcgctc
     8821 tccgtcttcg gcttctttaa ccccgatctg gcaaagctta ttaagtctgt cggcaaaaga
     8881 gcgggtgaaa ccgggcggca tatgctgggt aattaacagt gccggactgg aaagcggcaa
     8941 cggttgcagt acgtgacgaa ttgcctcagt tccacccgtt gaagcaccaa tcgcaatcag
     9001 tttttcagaa ctcaacaacg gccctgcctt cagcgttgtc ggtaccgaca atggcttatg
     9061 tgctgcaagg ctcgcctttg ctgctgtacg caccttttca gcaatcattt cgctatacgc
     9121 cagcattcct tcgcgaatac ccagttgcgg tttggtgaca aaatctatcg cccccagctc
     9181 cagcgcgcgc agcgtgactt ctgacccttt gccggtcagg gaagaaacca tcactacggg
     9241 cattggacgc aaacgcatta atttttcgag gaaatccagt ccgtccatcc gcggcatttc
     9301 aacatccagc gtcaatacat cgggattgaa tttcttaatc aaatcacgcg caaccagcgg
     9361 atcaggggcg gtcgccacca tttccatgtc gctatggctg ttgatgattt ctgtcatgat
     9421 ttggcgcatc agtgccgaat catcgacaga taacaccctg attttgctca tcgttaatcc
     9481 ttacttagcg catacaccgt ctgaccacgc agcgtgaagc ggcgctcaag gtggctaaag
     9541 ttttcagagt gacccgcaaa cagcaatccg tcgggtttaa ggagcggaac aaagcgacgc
     9601 aaaatctcct gctgggtagt ttgatcgaag tagatcatga cgttacgaca gaagatcgca
     9661 tcaaacggcc ccggcacggt gtactgtttc gccagtaaat tcagcggggc aaaatcaaca
     9721 tagttcgcca gctcctgacg cacgcgtacc agcccttcat gcggtcccgt ccctcgcatg
     9781 aaataccgtt gcagttgctg cggcgtcagg tttttcaact cttcatggcg ataaataccg
     9841 cttctggctt tttccagcac ttcggtgtcg atatcgctgg caaacacttt ccagcgtccg
     9901 ggcacggtgc tcaatgtttc agccagcgtc atcgcaatgc tgtacggctc ttcgccggtc
     9961 gaagccgctg cgctccatac gcgatactcg ccagaacggc gacgtgcgtg atccgcgagc
    10021 agaggaaaat gatgcgcctc acggaaaaat gccgtcagat tcgtggtcag cgaattgata
    10081 aacgcctgcc actcaccgct gtgctgatta gattccagca agttcaggta atggccgaaa
    10141 tccgtcagtc ccagcgaacg caaacgacga accagtcggt tgtaaaccat gtcgcgttta
    10201 tggtcggcca ggacgatccc ggctcgttga tagatcaatt gacttatccg ccgaaaatgc
    10261 gcgtcggaaa gcgccaggcg ctcggtcatc tgtaacaata aagacgtttg cccacagggc
    10321 agagatgaag tcatagcgcc ttctcaatca cttcaggata ccactggcgc aatttgtaac
    10381 tgcgccgact catgtcgtgc tacttcatgt tcttcaaggg taaataccgc cacacgcgac
    10441 gaaagatggt cggcctgatt cagtcgttgc aaatcgtcgc gaaatgccca aaagacatgc
    10501 cgttactgcc aatctgcaag atcccgcaga gaatcaaaat taaaaacagc gtggtcgaaa
    10561 ttcgaatacg attaaacatc aacgctcccc atcaggcggc aatgaccgct ttagtaaata
    10621 ctcgtcaaaa tgtttcccag tttggatctt gttcagcaat tcgcagtcgt ggctgtgccg
    10681 gtggttgctc actggcagga cgggatggtg tttgcggttt attggtgagt gggctggctg
    10741 ccagacggaa cgcggaaacc gcttgcgtta aacgactcgc ctgttcttcc agcgcagctg
    10801 cggcggcagc tgattcctgc accagcgatg cgttctgttg cgtgacgcga tccatttccg
    10861 aaaccgccaa tgcgacttga tcgatgccac ggctctgttc atccgatgct gatgcaatct
    10921 cgcccataat gtcagtcacg cgagtgacag cattgacgat attgttcatt gtttccccag
    10981 cgctttcgac cagcaccgaa ccggtatcaa cgcgcgagac ggagtcttca atgagggctt
    11041 tgatctcttt tgccgcctgt gcactgcgac tggcaagatt acgcacttca cccgccacca
    11101 cggcaaaacc acggccctgt tcacccgctc gcgcggcttc aacagcggca ttcagcgcga
    11161 ggatattagt ctggaaggca ataccatcaa taacgctgat aatgtcggca attttcttcg
    11221 aactatcggc gatctcatgc atcgttttca ctacgccatc caccactttg ccgccgtgct
    11281 gggcggtgtc ggaggcactt tgcgccagtt gcgaggcctg gcgggcgtta tcggcgtttt
    11341 gcttcactgt cgcggtgagc tgctccatgc tggcggcagt ttcttccagc gcggatgcct
    11401 gctgttcagt acgggaggaa agatcggtgt tgcccgccgc aatttcacgg gtaccggcat
    11461 agatggcatc tgaaccttcg cgcacatgag tgacggtgtc agtcaaagag cgttgcatat
    11521 gtgaaacgct ctgcgccagg tcgcccattt cactgcgccc gtcaatggtc agggtattcg
    11581 ccaggttacc accggcaatt tcgcgaatat gcgcaataat ttttgccagc ggtgtaagca
    11641 acatacggcg aatgccgtac cacgccacca gcagaatcaa taccaccacc agcgcgataa
    11701 ccgccagttg ccactgggca aatcggtaat catctgcgtt gtcagtgacg atatcgcgat
    11761 acagtttttc actgctgagg gcgtactgag caaacgcttc gcccattgca ttttgcattc
    11821 cctgggttgg ctgagcgaaa taagctccag tattgccata atcaagataa tcaatcagtt
    11881 cagttaacgc tgtgtgatag tttttatatt tttcatcaat attacgactg gtagcgacca
    11941 tttcaggtaa cggtgccatg cttttgaatt ttttataatg agtcgcggcc tgggccaatg
    12001 ttttcctggc gctatcgagc aattcaactt tggcattact ttgctgattg gatgaatcca
    12061 tcatcatccg caccgctgaa cgactcaggt taatgcgcgt ttgtagcatt aaatcccatg
    12121 ttgacgtcag ctcgccctgc tgttcccgta attgattgga aaccacaaag ctcttctggc
    12181 tatggtgaag ggaagaaaaa aacagactgc cggaaataag ctgtaacagt gcgaataccc
    12241 ccagcaccat taccaacagc gtgactacgc ggatacggtt aatcataagg caccttcctg
    12301 aaaacatgtt gatctcgtta tcggcaagga ggggggaaac tttattgctg atgccacccg
    12361 ccgcgaaatt gaaataaaaa acccgatgcg cagatcatcg ggttcatttc aattgaggaa
    12421 atcgggagaa ttacgccact tctgacgccg cgctatctaa cagcgccatc tcttcgctgt
    12481 tcagcagttt ttcgatgttc accagaatca acatgcggtc gcccagtgcg cccagtcctg
    12541 tgagatattc ggttgaaagc gtcacggcaa attccggtgc cggacgaatt tgctccgccg
    12601 tcaatgaaag cacgtctgag acgccgtcaa ccacgatgcc gaccacccgc tgtccgagat
    12661 tcaggacgat aactaccgtg ttgtcgttat agtccacatc cacctggctg aacttaattc
    12721 gtaagtcaac aatcggcaca ataacgccgc gcagattcgt gacgcctttg ataaacgctg
    12781 gcgtgttcgc aatccgtgtc acctgatcgt agccacggat ctcctgcact ttcagaatat
    12841 caataccgta ctcttcatca ccaagggtaa ataccagaaa ttcctggcct gacggctcgc
    12901 tggccagctt tgttacattc gtcataccgg tcatattgtt acctttttac tcattcaggc
    12961 ggcggtgttc gccatacgtt gttcgcggtt tatcgcctgc aaggcggaga catcaacaat
    13021 cagtgccacg ctgccgtcgc caagaatggt cgcagcagaa atgccgggga ctttgcgata
    13081 gttactttca aggtttttaa ccacaacctg gtgttgacca attaattgat ccaccagcaa
    13141 ggcgtagcgg cgaccgccac tttgtaagat caccacaatt ccctgggtgg cttcggtttt
    13201 cgcgcccgcg acgttgaaca ctttccacag ttcgacgata ggcagatatt caccccgcac
    13261 ttccagcacc cgctcgccgc cagccagtgg atgcagatcg gcttcacggg gttgcagtga
    13321 ttccatgaca gcattcagcg gcagaatgaa aacttcatcc gcaacgcgta cggacatgcc
    13381 gtcaagaatt gccagcgtca gcggcagtaa aatgcggatc gtagtgccag taccctgctt
    13441 cgactggatc tcgacatgac cgcccatctc ctggatatta cgtttaacga cgtccatgcc
    13501 gacgccacgc ccggagacgt cggtgacctg ctccgccgtg gagaagccag gtgcaaatat
    13561 cagcattgca acttcgtcgt cgctcatgtt ttcgctgacg gtcagacctt gcgaggccgc
    13621 ttttgccaga attcgttcac ggtttagccc cgccccatcg tcggtcactt caatgcaaat
    13681 gttgccgccc tgatgttcgg cagacagaat taaatttccg acgctgtttt tacctgcagc
    13741 gagccgtttt tctggcagtt caataccgtg atcgaggcta ttgcgtacca ggtgggtcag
    13801 cgggtcgata atgcgttcta tcaggctctt gtcgagttca gtggaactgc ccaccagcgt
    13861 cagttctacc tgcttgccga gttttcccgc cagatcacgc accagccggg gatagcgact
    13921 aaagacatat tccatcggca tcatgcgaat cgacatcact gattcctgca aatcacgagc
    13981 gttacgttgt aactgcccca tgctggtaat caaatcacca tgattaaccg ggtccagttc
    14041 actggagcgc tgagcaagca tggactgggt gatgaccagc tcgccgacga ggttaattaa
    14101 ttgatcaacc ttttctaccg ctacacggat gctggtggat tcactgctgc gcgtcgtttt
    14161 ttcccgctcc acgcgaccgg ttggcgcttg ttcggctgcc agtttaagca ctggtggggt
    14221 ggatattttt ggcgagacgt ctactgtttc aaaggtaatc tgatcggctt caatcacaaa
    14281 acagagtacc gcagtgatgt catcttcggc gatgtcgtcc ggtaatattg ccgagagaga
    14341 atcagcccct ttcaccacgt cagttaacgt tgtcagatgc cccaactctt cttccagcag
    14401 gtcgacttcg ccggccttca ggcgcgaaag gataattcgt cgcggtgact gactacgact
    14461 ctgctcatct tgcggttcac ttttggcaac cacacttaat cgggtcactg cagatggcgt
    14521 ttcgcctttc gcttctaatg ccagttgacg caaggcctgg cagatataat cgaagctggc
    14581 ggcatccgtc tcttgcgact gtttataagc gtcgagctgt tcttgcatga tgtccttcgt
    14641 ttccaaaaac agattgataa tgtcggtgtt gagttgcatc tcacctcgtc tggcttcatc
    14701 gagcaggttt tccatcagat gcgtggtttc ctgcaaaacg ctgaagccaa aggttcctgc
    14761 ccctcctttg atcgagtggg cagcccgaaa gatggcattc aactgttcgg catctggcgc
    14821 ttccggctgc aaaaccagca gatgctgctc catatcagcc aacagttcgt ccgcttcatc
    14881 aaaaaatgtc tgataaaaat cgcttatatc catgctcacg ctgtcacctc ggttcggctg
    14941 atggcattgt gggaacactg acctgtggtg caacctcagg tttttccagg gcgcttactg
    15001 gctcattctg gctttcggcg ttttcatgca aaatggcctg ttcggcgtgt ttgttcagta
    15061 ccagcaggct gatgcgacgg ttgacggcat catcaggtcc gcgatcgctt aagcgcatcg
    15121 ttgccgccat gccgacgaca cgtaacactt tgccgccatt caacccacca accatcagtt
    15181 cgcggcggga tgcattggca cgatcggcag aaagctccca gttgctgtaa cctttctcac
    15241 cgctggcgta gggaaaatca tcggtatgac ccgagaggct aattcggttg ggaataccgt
    15301 tcagtacggg cgcgatggcg cgcagaatgt cgcgcatata gggttcgacg tcggcactgc
    15361 cagttctaaa catcgggcga ttctggctat cgatgatctg aatacgtaga ccttcctgga
    15421 ccagagcgat tttgagatgt ggacgtaacg cccgcagttt cggatcggac tcaatcaact
    15481 ggtcgagatc gccacgcaat ttccgcaatc gactttgctc catgcgtttt ttcagctctt
    15541 cgatgttcgg ctgcttattc acttccccct ggctttgggt gtaatcatca cccccaccgg
    15601 gaattgggct ttcactatta gaaatgcgat cgccgcccgt aaccgctgtc gccagcggag
    15661 tccggaagta ctccgcaatc tgaatcagct cttttggact ggagatagag atcagccaca
    15721 tcaccagaaa aaaggccatc attgcggtca taaagtcggc ataagcaatc ttccacgatc
    15781 catgtgctgc cccgtggctt ttggctttgc gtcgtttgac gacaataatc ggatgcgctt
    15841 gattcttcat gcttcctcgg ttgtcgtctg ttgttgcgga tttttcaccg cacgcacatg
    15901 ctcttccagc tcaataaacg acggacgttc gctggaatag agcgttttgc gaccaaactc
    15961 aacggctata ggcggtgcgt aaccgttcag atttgaaagc agagtgactt tgacgcactg
    16021 catcattttg ctggtttcgg cgcttttctg acgtaaaaca ctcgctaatg gggaaataaa
    16081 tccgtaagcc aataaaatac cgaggaaagt ccccaccatc gcatgagcga taagcgcccc
    16141 cagctcggcg gcaggacgat cggctgaacc taacgcgtga actaccccca ttacagccgc
    16201 aacaatacca aacgccggaa gtgagtcccc gaccagcgcc agactgttcg ccgggacttc
    16261 tgcctcgctt tcgtgcgtct caatctcttc atccatcaga gcttcgattt cgaaggtgtt
    16321 catgtgacca ctgataatca ggcgcagata atcgacgata aattcaagca tgacgctatc
    16381 cgcaaggatg cgtgggtagc tggcgaagat ctcactctca cggggatttt caatatcgcg
    16441 ttccagcgaa aacatcccca tctgccgcga tttcgccatc aaccgataaa gcagagccag
    16501 cagatccata tacattgctt tggtgtattt ggagcgacga aacagcaacg gcagcgcctt
    16561 cagcgtgcct ttaatcgctt tgccattatt gccgacgata aacgacccaa tccctgcacc
    16621 ggcaataatc accagttcag cgggttgata gagtgctcca aggcttcctc cggtcatcaa
    16681 ataaccgccg aaaactgtac cgagaacaac caggtaacct aataagataa gcacgacatc
    16741 atccttccac tgttgaccat gacaggatgt tcagtcgtca ggcgttaaca cgcgataggg
    16801 acaaaaaaaa gcagcggtac atcgttaccg ctgctggaat gtttcgccac accgtatcag
    16861 ttaaacagcc tgtactctct gttcatccag cagttgtggg ataatatcgg caggattctg
    16921 ggaaagttta cgtcttttta ctgcccggga tggcggctga cataagctgc aggcaaagct
    16981 gccaacaggc tggtgagcat gggtaataaa attgccgccg cagcagttgc agctggaaag
    17041 ttgcagtaat ccactttcaa caaaccgcac caatgtccag gcacgggtta atgccagcag
    17101 cggtccttct tctgcttgcg ggcactgttc aaggtataaa cggtaggctt tgatcaccgc
    17161 atcgacgcca ttacacaagc cagttttcag taaaaactgc catgcattac agaacatcga
    17221 agcatgaacg ttttgttccc aggtcataaa ccagtcggtt gagaatggca gcatgccttt
    17281 cggcggtggg cttccgcgca gttctttata gagttttatc agacgtccgc gacttaactg
    17341 tgtttcgctt tccagcatct gcaaacgagc gcccagggtg atcaattcca ttgccagctg
    17401 aatatcccgc gcttcctgaa caatgctttt ttcactcatg atcaggccct tttcttacgc
    17461 agcgcttctt caggctgatt aacatcattc agcaagcgtg ttgagagcat gatgccggta
    17521 tgaatttgct ggagatcgtc aacgcgggaa tcttgcgtca actgagtaat cgtctggtgg
    17581 ctgtcaaaac ggaagtgaca aaccagttga ttggtttctg ccagcttaac catttgcgga
    17641 agagtcagtg ccgctaacgt tgtcgccatt tcttcattta tgccgagacg aaacatagcg
    17701 gacgctttgt cctgaacaat cagacgctgt gcaagtagta aatatgacaa gttgatgtca
    17761 taaatgtgtt tcagcaactc ggaggtatgc attattccca cccagaataa ccaactttat
    17821 ttttatgcgg tctcaccgca ccccgtgatg tcgccgggaa gccccggtaa aaaataatta
    17881 gcattagaat agttgcgata agctgcaata agcagaccca cctttttggt ttaataagtc
    17941 cttacaaata gaagggggtc tttacactta tctaagattt ttcctaaatc gacgcaactg
    18001 tactcgtcac tacacgcaca tacaacggag gggggctgcg attttcaata atgtgtgatg
    18061 cagatcacac aaaacgctca attacttaac ataaatgtat a
//
LOCUS       DAZGJT010000096        17898 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZGJT010000096 DAZGJT010000000
VERSION     DAZGJT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17898
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.096"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(8..1990)
                     /locus_tag="ACHKFK_003657"
     CDS             complement(8..1990)
                     /locus_tag="ACHKFK_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9053907.1"
                     /translation="MSIQTESLMTDALCGMLAADISLSRPVSSSDEYDKLSRSYHMNR
                     RNLAMLARPESGPLLSSGTRPPRDTPVDLLVWSLAVPDFTYEQRSRVVSLISEFGSFS
                     YTVAYAFSRLVYQCGVSNAVAVLESVHRYAFINADGDRYSVGLFCTDSELAVAADSIV
                     HDCLCSSAIVSEDEGVWLANVLHYFYAVSGFDFLPCLKAYMTRYGISGLLKRMTSLKF
                     VTRFLRVVRDQRVNEVCRMLGILNRTTPYISDWHLALFESRKDKVTRWLKNNGVFDFS
                     GELLCTLEDAHNSTVSNPVNRVAELCVRGKAVCELAEDFGLQGFFVVLTTPSRFHPTT
                     SYRAGGRWCSRQNPNWLAAGCPTVKDSHQWLNRVWQNIQRKLNKAGIQLPGIRTVEPH
                     ADGTVHWNFLFYCQPYDAEAVLNIFRDEALADNPGEPGAQDHRIKIKEIDPGKGGFRY
                     IVKYITKMAGHADAKGTGHLDDIHSSRSFSDAVNRVACWQKTTRLRLFQFFGLPSVTA
                     YRQLRRFRIPFSPDDIAMKKITPEQVEQLEKIRLSCDAGDFRTYILLNGGFFCSERLI
                     RPYYFQQVQDGMPRLNVYGEICAPVISGFLFNKVPFITRVFGLFISAIKELSVPVFNS
                     CVRTGDTLHTRPRRGGGGAAGRPWTWTCDNNFPVDT"
     gene            complement(2119..3072)
                     /locus_tag="ACHKFK_003658"
     CDS             complement(2119..3072)
                     /locus_tag="ACHKFK_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9053908.1"
                     /translation="MTESVKVNVGIDVSKDFLDVHVGNVALRRVPNRSSGLSVLKRIL
                     SRYSVSLVLCESTGGYENLAVTYFQSLGYDVCVINPRQARDFARSMGRLAKTDRIDAQ
                     ILCRLADVIDASPERTRFIRPLTDERRQHLVSMVRRRRQLCDLMTAERNRRAKTDDYG
                     SESISTVLDFLTDEVARIDRDIAAHVGSCFSDISSLLQTFTGIGPVTASTLLGELPEL
                     GKLNRRQITALVGVAPFNRDSGYMRGRRRISGGRSSVRNVLFMAALSSVRFNPVLKAF
                     FTRLVAAGKPKKVALVACMRRMVCILNAMLRDGSRFEVAVG"
     gene            3271..3642
                     /locus_tag="ACHKFK_003659"
     CDS             3271..3642
                     /locus_tag="ACHKFK_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO9053909.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            3748..4146
                     /gene="fadM"
                     /locus_tag="ACHKFK_003660"
     CDS             3748..4146
                     /gene="fadM"
                     /locus_tag="ACHKFK_003660"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO9053910.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWHGLENSDSFHWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(4198..4893)
                     /gene="queC"
                     /locus_tag="ACHKFK_003661"
     CDS             complement(4198..4893)
                     /gene="queC"
                     /locus_tag="ACHKFK_003661"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO9053911.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLK"
     gene            complement(4958..6658)
                     /locus_tag="ACHKFK_003662"
     CDS             complement(4958..6658)
                     /locus_tag="ACHKFK_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFO9053912.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDMGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSLRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLTGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            6758..7576
                     /gene="cof"
                     /locus_tag="ACHKFK_003663"
     CDS             6758..7576
                     /gene="cof"
                     /locus_tag="ACHKFK_003663"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO9053913.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            7729..8187
                     /gene="decR"
                     /locus_tag="ACHKFK_003664"
     CDS             7729..8187
                     /gene="decR"
                     /locus_tag="ACHKFK_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO9053914.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            8217..9989
                     /locus_tag="ACHKFK_003665"
     CDS             8217..9989
                     /locus_tag="ACHKFK_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9053915.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRTMLAEAPVVIDGSDKVPEGRGE
                     LDVNIRQFTYPQTDHPALENVNFALKPGQMVGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAIDA"
     gene            9982..11763
                     /locus_tag="ACHKFK_003666"
     CDS             9982..11763
                     /locus_tag="ACHKFK_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO9053916.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSTLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     EIVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDANTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLVGEELAASVREEESLSA"
     gene            11944..12282
                     /gene="glnK"
                     /locus_tag="ACHKFK_003667"
     CDS             11944..12282
                     /gene="glnK"
                     /locus_tag="ACHKFK_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO9053917.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            12312..13598
                     /gene="amtB"
                     /locus_tag="ACHKFK_003668"
     CDS             12312..13598
                     /gene="amtB"
                     /locus_tag="ACHKFK_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO9053918.1"
                     /translation="MKIATIKTGLTSLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTVPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNING
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(13647..14507)
                     /gene="tesB"
                     /locus_tag="ACHKFK_003669"
     CDS             complement(13647..14507)
                     /gene="tesB"
                     /locus_tag="ACHKFK_003669"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO9053919.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPDERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            14725..15297
                     /locus_tag="ACHKFK_003670"
     CDS             14725..15297
                     /locus_tag="ACHKFK_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO9053920.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(15328..15639)
                     /locus_tag="ACHKFK_003671"
     CDS             complement(15328..15639)
                     /locus_tag="ACHKFK_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO9053921.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQLYRWN
                     Y"
     gene            15802..15898
                     /gene="ffs"
                     /locus_tag="ACHKFK_003672"
     ncRNA           15802..15898
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKFK_003672"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            16018..16371
                     /locus_tag="ACHKFK_003673"
     CDS             16018..16371
                     /locus_tag="ACHKFK_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFO9053922.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(16413..>17898)
                     /gene="pdeB"
                     /locus_tag="ACHKFK_003674"
     CDS             complement(16413..>17898)
                     /gene="pdeB"
                     /locus_tag="ACHKFK_003674"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO9053923.1"
                     /translation="GLSIWLAHQQVETSFIEELNTYSSRVAIRANKVATQGKDALQEL
                     ERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPGKIS
                     KDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGNAHN
                     VVITSSDELAQGIITRLQKTPGEHIENNGIIYDILPLPEMNISIITWASTKMLQKGWH
                     RQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDVIENRDICVHHQPIVSLANGK
                     IVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQHPQQ
                     HISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISRYRE
                     AGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIKMAKTLKLKM
                     VAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT         4414 a         4464 c         4755 g         4265 t
ORIGIN      
        1 ttaatcctca tgtatcaaca ggaaagttat tgtcacaagt ccaagtccaa gggcggccag
       61 ccgccccacc gcccccgcgc cgggggcgcg tgtgcagcgt gtcacctgtc cgcacacagg
      121 aattaaaaac aggcacagac agttctttaa tcgcagaaat aaacaaacca aaaacacggg
      181 taataaacgg caccttatta aataaaaaac cggatattac aggggcgcag atttcaccat
      241 agacattcaa acggggcatg ccgtcctgaa cctgctgaaa ataatatggc ctgattaaac
      301 gttcagagca gaaaaaacca ccgttcagaa gtatataggt acgaaaatca cctgcatcac
      361 aggaaagacg aattttctca agctgttcaa cctgttctgg tgtgattttt ttcattgcaa
      421 tatcatcagg cgaaaacggt atcctgaaac gtcgcaactg gcggtaagca gtcacagaag
      481 gcagcccgaa aaactgaaaa agacgaaggc gggttgtttt ctgccagcag gcaacacgat
      541 tgaccgcatc actgaatgaa cgggatgagt gtatatcatc aagatgacca gtgccttttg
      601 catcagcatg acccgccatt ttggtgatgt acttgacgat atagcgaaaa ccgccttttc
      661 ccgggtcaat ctctttaatt ttaatgcggt ggtcctgagc acccggttca cccggattat
      721 ctgcaagtgc ctcatcgcgg aatatgttca gaacagcctc agcatcataa ggctggcagt
      781 aaaacaaaaa attccagtga acagtaccgt ctgcatgagg ctcaaccgta cggatacccg
      841 gcaactgaat ccccgccttg tttaatttac gctgaatgtt ctgccagacg cgattaagcc
      901 actgatgcga atccttaacc gtagggcacc cggcagccag ccagttcgga ttctggcggg
      961 agcaccaacg gcccccggct ctgtaactgg ttgtcggatg aaaccgtgac ggtgtggtaa
     1021 gcacaacaaa aaacccctga agaccaaaat cttcagcaag ctcacagaca gccttacccc
     1081 gaacgcaaag ttcagccaca cggttaaccg gatttgaaac cgttgaatta tgagcatctt
     1141 ccagtgtgca gagcagctca ccactaaaat caaacacacc gttatttttc agccatcggg
     1201 taactttatc tttgcgactt tcaaataaag ccagatgcca gtctgaaata taaggggtgg
     1261 tgcggttaag tattcccagc atgcgacaga cctcattcac ccgctggtca cgaacaacac
     1321 gcaggaaacg agtcacgaac ttaagcgagg tcatacgttt aagaagaccg gaaatgccgt
     1381 aacgtgtcat gtatgctttc aggcacggca ggaaatcaaa accggaaacg gcataaaaat
     1441 aatgcagcac gtttgccagc cagactccct catcctctga gacaatggca gaagaacaca
     1501 gacaatcatg cacaatactg tcagccgcaa ccgccagttc agaatcagta cagaacagac
     1561 cgacgctgta acggtcacca tcagcattaa taaaggcgta acgatgtaca gattcaagaa
     1621 cggcaacagc gttactcact ccgcactgat aaaccagacg cgaaaaagca taagcaacgg
     1681 tataactgaa tgaaccaaac tcactgatta gcgacaccac acgggagcgc tgctcataag
     1741 taaaatcagg tacagccagc gaccagacaa gaaggtctac aggtgtgtca cggggcggac
     1801 gtgtgccgga agaaagcaaa ggaccggatt cagggcgcgc cagcatggca agattgcgac
     1861 gattcatgtg atacgagcga gacaatttat cgtactcatc agaagacgaa accggacggg
     1921 ataatgagat atccgccgca agcatgccac aaagggcatc agtcatgagg gattcagttt
     1981 gtatactcat aatcagttac cggatttcag gaggcaaaaa atcgagcgcc caagaaacac
     2041 aatacccaca cccaaaaaga ccaacataac caaacagaac agttccaagg tttccatcgt
     2101 ttacgaatct ccgcacactc accccacagc cacctcaaaa cgggaaccgt cacgaagcat
     2161 ggcgttaagg atacagacca tgcggcgcat acaggcaacc agtgccacct ttttcggttt
     2221 cccggcagca acaagacggg taaagaacgc cttaagtacc ggattaaaac ggacggatga
     2281 cagcgccgcc ataaacaaca cattccgcac gctagaacgc ccgccagaga ttcgacgacg
     2341 accacgcata taacctgaat cacggttaaa cggagccacg ccaacaagag ccgtaatctg
     2401 acggcggtta agctttccca gttcaggcaa ttccccgagc agggtgctgg ctgttactgg
     2461 accaatacca gtgaatgtct gaagaagtga ggaaatatca gagaagcagg aacccacatg
     2521 cgccgcaata tcacggtcga tacgggccac ttcatcggtc agaaaatcaa gcactgtact
     2581 gatactttct gaaccgtaat cgtccgtttt ggcccggcgg ttacgctcag ccgtcatcag
     2641 gtcacagagc tgacgacgac ggcgaaccat cgacaccaga tgctgacgac gctcatcagt
     2701 cagcggtctg atgaaacggg tacgctcagg cgatgcatca ataacatcag ccagacgaca
     2761 aagaatctga gcatcaatcc ggtcagtttt ggcaaggcga cccatggaac gggcaaaatc
     2821 acgcgcctgt cggggattaa tcacacagac atcatagcca agcgactgaa aatacgtcac
     2881 cgctaaattt tcataccccc cggtggactc acacaggaca agagaaacac tgtaacggga
     2941 caaaatacgc ttcagaacag acaaaccgct ggagcggtta ggaacacgac gcagggccac
     3001 attacccaca tgtacatcca gaaaatcctt actgacatca ataccaacgt taactttgac
     3061 agattcagtc atgtttaacc cttcctagtg tgcgaacttg attcggtaaa cgtaattcga
     3121 tcaacaaata cgggcttgaa caaaaataag aaccatgcgc tataggcttg tttacgggct
     3181 aaaaccccag gagaaatcag gctgcatggt tcttttctgt acagcaggaa ggagtataaa
     3241 cacagatcaa acacactagg agaaaacagt atgaaacacg gaattaaagc actgctcatt
     3301 accctgtccc tggcctgtgc cggaatgtct catagcgcgc tggcggcagc ttctgtggcg
     3361 aaaccgacaa cagtagaaac caaagcggaa gctcctgcag cacaaagtaa agcagcagta
     3421 ccggcgaaag ccagtgacga agaaggcacc cgggtcagca ttaataatgc cagcgcggaa
     3481 gagttagccc gcgcgatgaa tggcgttggc ctgaagaaag cgcaggcgat tgtcagttat
     3541 cgcgaagagt acggtccgtt taaaacggtg gaggatctaa agcaggtgcc ggggatgggc
     3601 aattcgctgg tggaacgtaa tctggcggta ttaactctgt aattaatttg catagtggca
     3661 attttttgcc agactgaaga ggtcatacca gttatgacct ctgtacttat aacagttctt
     3721 ataacaacaa cgtaaggtta ttgcgctatg caaacacaaa tcaaagttcg tggatatcat
     3781 ctcgacgttt accagcacgt aaacaacgcc cgctaccttg aatttctcga agaagcccgc
     3841 tggcatgggt tggaaaatag cgacagtttt cactggatga cggcccataa catcgccttc
     3901 gtcgtggtca atatcaatat taactatcgc cgcccggcgg tattaagcga cctgttaacc
     3961 attaccagtc agttgcagca attaaacggt aaaagtggca tcttaagcca ggtcattaca
     4021 ctggagccgg aaggacaggt ggtagcggat gcgcttatta cgtttgtttg tattgatctt
     4081 aaaacgcaga aagcattagc tctggaaggg gaattgcgcg aaaagctgga gcagatggtt
     4141 aagtaaacgt tttgtggtgc cggatgctca agccgcatcc ggcgacaccc ggaataatta
     4201 cttcaacccg gttttctgct tcatcgctgc catcaccgtc ggtttatcgg ccagataatg
     4261 attcaaaccg ttggcacgta aattacacgc cgcacaatga ccgcaaccgt cgcctttaat
     4321 gccgttataa caggtcagcg tttcgttacg gactaaatcc agcttgccgt aataatctgc
     4381 cagcgcccag gtttccgctt tatcaatcca catcagcggc gtttcaaaac gaatatcttt
     4441 cgccatgccc aaactgacgg catggtttag tgctttcaca aactcatcgc ggcaatccgg
     4501 gtagccggag aaatccgttt cgcagacgcc ggtaattacg gcttctgctt ttacctgata
     4561 cgcatatatt gccgccagtg tcaggaacaa aatattacgc cctgggacaa acgtattcgg
     4621 gataccatcg gcttcaggtt cataatcagg caccggaatg ctgtcacgcg tcaggctgct
     4681 gaccgccagc tcgttgagca gcgttacatc aagcacctta tgcgcgcgtg cccccagttt
     4741 cagcgccagt tcgcgtgcca cgtcgatttc tgcgcgatgg cgttgaccgt aatcgaacgt
     4801 cacgcaatgg acctcatcat attgttgtaa tgcctgcacc agacaggtgg tggaatcctg
     4861 gcctccactg aacacaacga cagcacgttt catagataat cctgcttgaa caataaaagc
     4921 gttatggtaa cgcctgcgat taacccggac cagctattca ttgcgacggc gcgggaagcc
     4981 aggcttcggt aaattcaaac cagccgcgcg gtgtcagtcg cacaccgttc acgccgggag
     5041 gggcgctaat gcgatagtga tagttgaaca gcggcgtcag cgtcgcatct gccattaact
     5101 ggctaaaaac cgctttcagg gcattaaatc ggttttcctc atcaggcatt acttgcactg
     5161 cgtccagtgt cgattgcaaa tgtgcatatg ctggagcgtc gaaaacatgt ggccacagtg
     5221 gatcgcaacg cagccattgc tccagggtat attccggtgc ttcgccaatt aatctgtcgc
     5281 ccatcatgag gtctgcgtgt gccagtaggg tcgtgtcgtc ccagtttttt gcgttatgaa
     5341 aaataattgt gagttcacag ccttccgctg ccagtgtcgc ctgtaggcgt tctgccatgg
     5401 tatgaagttc tatcggtagg tgataaacca gcgtcaaggt tttcggtagt ttaacttcat
     5461 ccggtacttg ccaatgcgga atagtccagc ctgtcagtaa tgcatgactg gcagtgatca
     5521 ggttttctcc gacttctaac gtttgtaata aaccggattg atgaataatg gagattactt
     5581 ttcgcgcctg ccagagggac agtcggagac ttttgcgcaa tgttaaatag caaaaaccta
     5641 aactgatgcc gctactgacc tggctgaccc gttgcagctc ctccggtttg ccaatggtga
     5701 tttgcacggg atgccgacaa ctggttccca tatctttttc gaaaagcggc ggagttatcc
     5761 agtactcaac cgctttaagc agcggatgac gtaaatggta ataatcatgg ctctccaggc
     5821 gcaccagttc cgctgtgaat tgtgttaagc gaaaaggacc tgtgccgatc agtgggaatt
     5881 gcggatgcgc cagatggctg caatagctcg ccagtcggtg cgcaagccag taatcggggc
     5941 gatgtaaaaa gaaggtcaga cactgcggat gggtgacttc aatacgcttc acgctaataa
     6001 ataattgatc cagtgctggc agttgtaaca gcatcaataa ccgctggtgt aagtgggagg
     6061 cttttactgc atcgccgtta tgccagtgta aggttgagcg aagataaaaa tcccagcgta
     6121 acccgtcagt agaggtttcc cagtgatgcg ctaaatcgcc aatcgggcgc tgggtattat
     6181 tatcgaagcg ggtcaggccg gaaaatatct gcccggccag atgctgctcg gcacggccgg
     6241 gcaaaaagcc cggttgtagc ggttcgagcg ggcgatagta gggaatacgc aacgtgggcg
     6301 tatcgttttg ccattgtccg cccataaacg gctgtaacag agtgcgcagc tcacctgggg
     6361 ccagttgcgc cagctccagc acatcttgct gctttccggt ttccagcgcc tgttccatca
     6421 tcgcattgcg tagcgattct ggcgtgacca gaaagcgtaa ttgtccgcgc tttccgcgtc
     6481 ctgactgcgc ctgccactcc agccatcccg cctcctgtgc ctggcgtaac agcgtacgaa
     6541 catggcgttc gctgcaaaaa cagcgttcgg ccagttcgct gacggtgacg gtttgcggct
     6601 ttccggcgga aggttgccac agacgttgat actggttaag acggttgagc agtcgcatat
     6661 aaacccggaa caatattatt taactattca ctattacttc cgtatatatc aggtgatact
     6721 caatcaccat taaccgtgtc acagagtgga gaaagaaatg gctcgtctgg cagcatttga
     6781 tatggatggc actttattga tgcctgacca tcatttaggt gagaaaaccc tctctacttt
     6841 ggcgcggctg cgtgaacgcg acattaccct cacttttgcc acggggcgtc atgcgctgga
     6901 gatgcagcat attctcgggg cgctatcgct ggatgcatat ttgattactg gcaacggaac
     6961 gcgcgtgcat tctctggaag gtgaactttt acatcgtgat gatttacctg cggatgtcgc
     7021 ggagctggtg ctgtatcagc aatgggatac ccgagcaagc atgcatatct tcaatgacga
     7081 tggttggttt accgggaaag agatccctgc gttgttgcag gcatttgtct atagcggttt
     7141 tcgttatcag ataatcgatg tcaaaaaaat gccactcggc agcgtcacca agatctgctt
     7201 ctgtggcgat cacgacgatc ttacacgctt gcagatccag ctatacgaag cattaggcga
     7261 gcgtgcacat ttatgttttt ccgccacgga ttgcctcgaa gtgctgccgg tgggttgcaa
     7321 taaaggcgct gcattgacgg tgctgaccca acatttaggt ttatcgttac gcgattgcat
     7381 ggcctttggt gatgcgatga acgatcgcga aatgttaggc agcgtcggta gcggatttat
     7441 tatgggcaat gcgatgccgc aactgcgcgc ggagctcccg catttaccgg tgattggaca
     7501 ttgccgaaat caggctgtct ctcactattt gacgcactgg ctggactatc cacatctacc
     7561 ttattccccc gaataacgag atcccttcca gcaccgggca attgcccggt tttttttgcg
     7621 ttgaatttgt cattttgtgc cgtgatgctt aaaccgcaca gaataaattg tcgcgatttc
     7681 acctttaaaa tagaattaaa agagaaaaaa attctctgtg gaagggctat gttagataaa
     7741 attgaccgta agctgctggc cttgctacag caggattgca ccctctcttt gcaggcactg
     7801 gctgaagccg ttaatctgac aaccaccccc tgctggaagc gtctgaaacg gctggaggac
     7861 gacggtatcc ttatcggcaa agtcgccctg ctggatccgg aaaaaatagg cctcggcctg
     7921 accgcctttg tgctgataaa aacgcaacat cacagcagcg aatggtattg ccgctttgtc
     7981 acggtggtta ccgaaatgcc agaagtgctg gggttctggc gcatggctgg tgaatacgat
     8041 tatctgatgc gcgtccaggt tgccgacatg aaacgctacg acgagtttta taagcgtctg
     8101 gtaaacagtg taccggggct gtcggacgtc acttccagct tcgcgatgga acagattaaa
     8161 tacaccactt ctttacccat cgaataaata tccagaatca agtcaggaca caacgcgtgc
     8221 gattatttgc tcaattaagc tggtatttcc gtcgggaatg gcgtcgctat ctcggggctg
     8281 tcgccttgct tgtcattatc gcgatgctgc aactggttcc gccaaaagtg gttggtattg
     8341 ttgttgatgg tgtgacagaa caacacttta ctaccgggca gatcctgatg tggatcgcca
     8401 ccatggtgct gattgccgtt gtggtttatc tcctgcgtta cgtctggcgc gtattgctgt
     8461 ttggtgcgtc ttatcaactg gctgttgaac tgcgtgaaga ttattaccgc cagctaagcc
     8521 ggcagcatcc tgagttttac ctgcgtcatc gcacgggtga cctcatggct cgtgcgacca
     8581 atgacgtcga tcgcgtcgtg tttgccgccg gagaaggggt gctgacgctg gtggattcac
     8641 tggtaatggg ctgcgctgtg ttgattatga tgtctacgca aattagctgg cagttgacct
     8701 tatttgccct gttgccgatg ccagtgatgg cgatcatgat caagcgcaac ggcgatgcct
     8761 tgcatgaacg ctttaagctg gcacaggcgg cgttttccag tcttaatgac cgcacccagg
     8821 aaagcctcac cagtattcgc atgatcaaag cctttggtct ggaagatcgt cagtcggcgt
     8881 tatttgccgc ggatgccgaa gataccggca aaaaaaacat gcgggtggcg cgtattgatg
     8941 ctcgtttcga cccgaccatc tatatcgcga ttggtatggc gaacttgctg gcgattggcg
     9001 gcggtagttg gatggtggta cagggcagtt taacgctggg ccagctcacc agttttatga
     9061 tgtatttagg tctgatgatt tggccaatgc tggcgctggc atggatgttt aacattgtgg
     9121 aacgtggcag cgcggcgtat agccgaattc gtaccatgct ggccgaagcg ccagtcgtta
     9181 ttgatggtag tgataaggtg ccagaaggtc gtggtgaact ggatgtgaat attcgccagt
     9241 ttacgtatcc gcagactgac catcctgcgc tggaaaacgt caatttcgcc ctgaaacccg
     9301 gtcagatggt aggtatttgc gggccgactg gttccggcaa aagtaccctg ttgtcgctca
     9361 ttcagcgtca tttcgacgtc agcgaggggg atatccgctt tcatgatatt cctctgacga
     9421 agttacaact cgatagctgg cgtagccgcc tggcggtggt cagccagaca ccgttccttt
     9481 tttccgacac cgtagcgaac aacatcgcgt tgggttgccc gaatgccacc cagcaagaga
     9541 ttgagcatgt cgcgcggtta gccagcgtgc atgacgatat tttgcgttta ccgcaaggtt
     9601 acgatacaga agtgggcgag cgcggagtga tgctttccgg tgggcaaaaa cagcgtatct
     9661 ccattgctcg tgcgttatta gtcaacgcgg aaatcctcat ccttgatgat gcgctttcgg
     9721 cagtagacgg acgcacagag caccagatcc tgcataacct gcgtcagtgg gggcagggaa
     9781 gaacggtaat catcagtgcc catcgccttt ctgcactgac ggaagccagt gaaattattg
     9841 tgatgcagca cggacatatc gcccagcgtg gcaatcatga tgagctggcg caacaaagtg
     9901 gctggtatcg cgatatgtat cgctatcaac aactggaggc ggcgctcgac gacgctccgg
     9961 aaattcgcga ggaggccatc gatgcgtagt tttagccaac tgtggccgac tctcaagcgc
    10021 ctgttagcgt acggttcacc gtggcgtaaa ccgttgggga ttgcggtcct gatgatgtgg
    10081 gttgcggcgg cggcagaagt cagtgggccg ctgcttatca gctattttat cgacaatatg
    10141 gtagcgaaaa ataacctgcc gttgaaagtg gttgcagggc tggcggcggc gtatgttggg
    10201 ctgcaactgt ttgcagccgg gctacattac gcgcagtcgc tgctgtttaa tcgggcggca
    10261 gtaggcgtag tgcaacagtt gcgtaccgac gtgatggatg ctgcgttacg ccagccatta
    10321 agcgagtttg atacccaacc cgtcgggcag gtgatttccc gcgtcactaa tgacactgaa
    10381 gtgatccgcg atctctacgt taccgtagtg gcaactgtcc tgcgtagcgc cgcactggtg
    10441 ggcgcgatgc tggtggcgat gttcagcctc gactggcgaa tggcgctggt ggcgataatg
    10501 attttcccgg tggtgctggt ggtaatggtg atatatcagc gttacagcac gccgattgtc
    10561 cgtcgtgtgc gcgcttatct ggcggatatc aacgacggct ttaacgaaat catcaatggc
    10621 atgagcgtta tccagcagtt ccgtcagcag gcgcgatttg gcgaacgtat gggtgaggcc
    10681 agtcgttcac actatatggc gaggatgcaa accctgcgcc tcgacggttt tctgctgcgt
    10741 ccgctgctga gtctgttttc atcgctcatt ctttgtggct tgttgatgct gtttggcttc
    10801 tccgccagcg gcaccattga agtgggcgtg ttgtatgcgt tcatcagtta tttggggcga
    10861 cttaacgaac ccttaatcga actgaccacg cagcaggcga tgctgcaaca ggctgttgtt
    10921 gctggtgagc gcgtttttga gctgatggac ggaccgcgcc agcaatatgg caatgatgat
    10981 cgcccgttgc agagcggcac catcgaagtc gataacgtgt catttgctta tcgcgatgac
    11041 aatctggtgc taaagaacat taatctctct gtgccttcgc gcaactttgt ggcgctggtc
    11101 gggcataccg gcagtggcaa aagcaccctc gccagtttat tgatgggcta ttacccgcta
    11161 acggaaggtg agattcgcct tgatggtcgt ccattaagta cgctaagtca cagcgcgctg
    11221 cgccagggcg tggcaatggt gcagcaagat ccggtggtgc tggcggatac cttcctcgcc
    11281 aacgtgacgc tgggacgtga tatctctgaa gaacgcgtct ggcaggcgct ggaaatcgtg
    11341 cagctggcgg agctggcgcg tagcatgagt gatggtattt acacgccgct tggcgagcag
    11401 gggaataatc tctcagtcgg gcaaaagcaa ctgctggcac tggcgcgcgt gctggtggag
    11461 acgccgcaaa tcctgatcct tgatgaggca accgccagca ttgactccgg gactgaacag
    11521 gcgattcaac atgctctggc ggcggtgcgt gaacatacta cgcttgtggt gattgctcac
    11581 cgcttatcga ctattgttga tgccaacacc attctggtgc ttcatcgtgg gcaagccgtg
    11641 gagcagggca ctcaccagca actgctggcg gcccagggac gatactggca gatgtatcaa
    11701 ctgcaacttg tgggcgaaga gctggcagcc agcgtgcgtg aagaggaatc attgagcgcc
    11761 tgaatagcgc aatatttcat cgttggtgca aaaatgtaac gcactgtgca ctgtcatagt
    11821 gcgttttcat tttcaatctt cttaacttcc tgctctcttt ctcgtttttc atttttggca
    11881 caccgcttgc aataccttct tcgtgtagca gaaccattac cgaattctga ccggagggga
    11941 tctatgaagc tggtgaccgt gattatcaaa ccattcaaac ttgaagacgt tcgtgaagcg
    12001 ttatcttcca ttggtattca gggcctgacc gtcaccgaag tgaaaggttt cgggcgtcag
    12061 aaagggcatg ccgagctgta ccggggggcg gaatacagcg tcaacttcct gccaaaagta
    12121 aaaattgatg tggcgattgc tgatgaccaa ctcgatgaag tgatcgatat cgtcagcaaa
    12181 gcggcttaca ccggaaaaat tggcgacggc aaaatcttcg tcgctgaatt gcaacgcgtc
    12241 attcgtattc gtaccggcga agccgacgaa gcggcgctgt aatctctggc acacagcaac
    12301 aggaacgaaa aatgaagata gcaacgataa aaactgggct tacttcactg gcgatgctgc
    12361 cgggactggt aatggctgca cctgcggtgg ccgataaagc cgacaatgcg tttatgatga
    12421 tttgtactgc gctggtgctg tttatgaccg ttccggggat tgccctgttt tatggagggc
    12481 tgattcgtgg caaaaacgtg ctgtcgatgc tgacgcaggt gaccgtgacg tttgcactgg
    12541 tctgtattct ctgggtggtt tacggctact cgctggcgtt tggcgaaggc aacaacttct
    12601 tcggcaacat taacgggttg atgctgaaaa acattgaact gacggcggtg atgggcagca
    12661 tttatcagta tatccacgtg gcgtttcagg gatcgtttgc ctgcattacc gtaggcttga
    12721 tagttggggc gctggcggaa cgaatccgct tctcagctgt gttgattttt gtggtggtat
    12781 ggctgacgct ctcctacatt ccgattgcgc atatggtctg gggcggcggt ttgctggctt
    12841 ctcacggtgc actggatttc gcgggtggca cggtggtgca cattaacgcc gcaatcgccg
    12901 gtctggtggg cgcgtatctg ataggcaaac gcgtgggctt cggcaaagag gcgtttaaac
    12961 cgcacaacct gccgatggtc ttcaccggga ctgccattct ctatatcggt tggtttggct
    13021 ttaacgccgg gtcagcgggc acggcgaatg aaatcgcagc actggcattt gtgaatactg
    13081 tggtcgcaac ggcggcggca attcttggct ggatcttcgg tgaatgggcg ctgcgtggta
    13141 agccttcact gctgggagcg tgttcaggcg cgattgccgg tctggtcggc gtgacgccag
    13201 cgtgtggcta tattggtgtt ggcggcgcgt tgattatcgg cgtggtagct ggtctggcgg
    13261 gcttgtgggg cgttaccatg ctcaaacgct tgttgcgggt ggatgatccc tgcgatgtct
    13321 tcggtgtgca cggcgtttgt ggcattgtcg gctgtatcat gaccgggatt tttgctgcca
    13381 gctcgctggg cggcgtgggc ttcgctgaag gtgtgacgat gggccatcag ttgctggtac
    13441 agctggaaag catcgccatt acgattgtct ggtctggtgt ggtggcattt atcggctaca
    13501 aactggcgga tctgacggtt ggtctgcgtg taccggaaga gcaggagcga gaagggctgg
    13561 acgtcaacag ccacggcgag aatgcctata acgcgtaaca agcactgcaa aaaacagccg
    13621 gacggttttc acctccggct atttttttaa ttgtgattac gcatcacccc ttcctgaacg
    13681 gtcgaggcaa ccagcacgcc gtcctgggta taaaactcac cgcgcacaaa gccgcgtgcg
    13741 ctggacgccg aggtgctttc cacgctatac agcagccatt cattcaaatt aaacgggcga
    13801 tggaaccaca tggaatggtc aatggtggca atctgaatcc ccggttcgag aaaaccgatg
    13861 ccgtgcggct gtagagctac cggcaggaaa ttaagatcag aagcataacc gagcagatac
    13921 tgatgaacgc gcagatcatc cggcacgcta ccatttgcgc ggatccacac ctgacgatgc
    13981 ggttctgcga cgtgaccttt cagtgggtta tgaaactcca ccggacggac ttccagcgga
    14041 cgatcgcaga tgaatttatc tttcagcact ggcggcagca ggtgcgccag cgattgggcg
    14101 atttgcgttt ctgaaggcag gccatcaggc gctggcgcgg acggcattgt tttttgatgt
    14161 tcgaaacccg cttctggtgc ctggaaagag gcagtcatat aaaaaatcgg tttgccgttt
    14221 tgaatagcag caacccggcg ggcgctgaag ctgttaccgt cacgcagcgt ttcgacatca
    14281 taaataatcg gcttcttact atcgccaggg cgaagaaagt agctgtgaaa agaatgtacc
    14341 agccgctcgt cagggacggt ctcttttgca gcatacaagg cctgacccac gacctggccg
    14401 ccaaatacct ggcgtaaacc taaatcttca ctctggccgc gaaagagtcc ttcctcaatt
    14461 ttttccagat ttaacaatgt cagtaaattt tttagcgcct gactcatata actctccagt
    14521 aacaaagctg ccgcagcaag ccaaagtgag ttgagtataa cgcaaatttg ccactggtcc
    14581 gatgggtgca atggtctgaa ttacgggcta attacaggca gaaatgcgtg atgtgtgcca
    14641 cacttgttga cgttactatt ttgttaacca ctcttccggc gaggaaagtt agcccgctgg
    14701 tgcattgata ataaggagaa gtgaatgaaa ctcgtgcaca tggccagtgg tttagcggtt
    14761 gcgattgcgt tggcggcttg cgcagataaa agcgcggata ttcagacgcc agcaccggct
    14821 gcaaatacgt ctatttcagc aacacaacaa cctgctatcc agcaaccgaa tgtctccggt
    14881 accgtttgga tccgtcagaa agtcgcactg ccgcctgatg ccgtgctgac cgtgacactt
    14941 tctgacgcgt cgttagccga tgcgccgtcg aaagtgctgg cgcagaaagc ggtgcgtacc
    15001 gaaggtaaac agtcaccatt cagctttgtt ctgccattta acccggcaga tgttcagccg
    15061 aacgcgcgta ttctgttgag tgcggcgatt accgtgaatg acaaactggt atttattacc
    15121 gataccgttc agccggtgat caaccagggc ggaactaaag ccgacctgac attggtgccg
    15181 gtgcagcaaa ccgccgtgcc ggttcaggcc agcggtggcg caacgactac cgtaccttca
    15241 acttcaccaa cccaggtgaa tccgtcttcg gcagttccag ctccaacgca atattaagcg
    15301 aagttaaccc tctccgcccg gagagggtta gtagttccag cgataaagct gcaagtcgat
    15361 ttgcccgctt cccgacacca tcacaccttc tgccagtaat gcctgtcgct gacgctgtaa
    15421 atccggtccg gtcagtgaaa tggtgccgtg gcgattaacc acccggtgcc atggcaaggt
    15481 gctgccttcg gggagacgct ttaacacacc gcctacctgg cgcgcggcgc gcggcgatcc
    15541 cgccagtttc gccacatcac cgtaagtggt gacatagcct tcgggaatag cggctacgat
    15601 ttgccagacg cgctggggaa atgaatcttc tttttccatc ttttcttcct gaggtaattt
    15661 ttcagcataa tctggaaaaa cgcccgagtg aagccgcatt gcgcaagaaa ccagcatctg
    15721 gcacgcaatg ggttgcaatt agccggggca gcagtgataa tgcgcctgcg cgttggttct
    15781 caacgctctc aatgggggct ctgttggttc tcccgcaacg ctactctgtt taccaggtca
    15841 ggtccggaag gaagcagcca aggcagatga cgcgtgtgcc gggatgtagc tggcagggcc
    15901 cccacccatt tctgcctccc accgtttcgt caaaaaatcc caacatggct aaactttaac
    15961 caccactgac gtcgcaagaa ttgtctggct gcgcagtacg cttcggaggt atgtctgatg
    16021 aagtatgttg atggttttgt tgttgccgtt cctgccgata aaaaggatgc ctatcgggaa
    16081 atggccgcta aggccgcgcc attatttaaa gagttcggtg cgcttcgtat tgtcgaatgc
    16141 tgggccagcg atgtaccgga tggtaaagtg accgattttc gtatggcggt gaaagcggaa
    16201 gagaatgaag aggttgtctt tagctggatt gaataccctt caaaagaggt ccgcgacgct
    16261 gctaatcaaa agatgatgtc ggacccacgg atgaaagagt tcggcgagtc catgccgttt
    16321 gacggcaagc gaatgatcta tggtggattc gaatcaatca tcgacgagta ggtagcgtga
    16381 cgggctgtgc ggcgtgccgt cagccctaaa attcacaaat gttgctcggc ccagcgtaag
    16441 aaatcctctt tcggtaatgc cttgctgtag agccagccct gaccgtagtg cacgccatgc
    16501 tggcgtagcc actcttcttg tttactggtt tcgattccct ccgcaaccat tttcagtttc
    16561 agagtttttg ccattttgat gatatgcggc gtgacatttt tatattccag cgcatcaacg
    16621 aaagatttat cgatcttcag aatgtcgaca tccagatcct gtaaatagct taaacttgaa
    16681 taccctgtac caaaatcatc gagataaatt tcatggcccg cctcccggta gcgagaaatt
    16741 atcggggcgc tggttttcgg atcggcaaac tcgcgttcag tgagttcaag cgcgatctgt
    16801 ctgggattaa cctgatagtg attgatcatt tcacgcagca attgcgggat tttttccgag
    16861 gtgagcacgg tggattcaag attgatcgaa atatgctgct gaggatgctg acgcagccag
    16921 tcgcccatat cttcaaagac gcttcttata atcagtagcg tcaatggctc agaaaggccc
    16981 gtttgctgtg ccagcggaat aaaactatct ggtgacaacc aactaccgtc tgtctgcggc
    17041 cagcgcgcca gtgcctcagc accgacaatt ttgccattgg ctaaggagac aatcggctga
    17101 tggtgcacgc aaatatcacg attttcgata acatcctgca gccgatgatg cggtgactgg
    17161 atacggcgca aaatacgcag cacaaacatc gctgccagca ggccaatcac caacccgagc
    17221 ggtaaccaaa taaagacttg ccgatgccag cctttctgca acattttcgt tgaagcccag
    17281 gtgatgatcg aaatattcat ctccggtaag ggcaggatat cgtaaatgat tccattattt
    17341 tcgatatgct caccgggtgt tttttgtagc ctggtaataa ttccctgagc aagttcatcg
    17401 ctgctggtta tgacaacgtt atgggcattg ccaataatgg cggcatcaat ttgccatgag
    17461 ctatagggaa tgacatcaat aaaggaagcg gggtcgatca tgacgacata atgtgccgtt
    17521 cccatggcga ccatgtaacg aataatgcct aaatcgttat gcgatgttaa ccagacacga
    17581 taaccatctt tcgaaatttt acctggctcg gggaaggtat cgggcgggct ttcatgctcc
    17641 agagacgaac actggggaac gttgttatcg atataaacca cttcctgaat atagcgataa
    17701 ctgtaagata cccgacgcat ttccatgaga tgggcttcgc tacaggcagc gccttgccat
    17761 ctttccagct cttgcagcgc atctttccct tgtgtcgcca ccttattggc ccgaatagcg
    17821 acgcgggagg aatacgtatt cagctcttca ataaatgatg tttctacctg ctgatgggcc
    17881 agccagatgc ttaagccg
//
LOCUS       DAZGJT010000097        17718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZGJT010000097 DAZGJT010000000
VERSION     DAZGJT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.097"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHKFK_003675"
     CDS             <1..480
                     /locus_tag="ACHKFK_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9053924.1"
                     /translation="GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAA
                     YLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAF
                     TLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            636..2171
                     /gene="gadC"
                     /locus_tag="ACHKFK_003676"
     CDS             636..2171
                     /gene="gadC"
                     /locus_tag="ACHKFK_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO9053925.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDAKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKSKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2302..3621
                     /gene="digH"
                     /locus_tag="ACHKFK_003677"
     CDS             2302..3621
                     /gene="digH"
                     /locus_tag="ACHKFK_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO9053926.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTPQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPEVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3999..4163
                     /locus_tag="ACHKFK_003678"
                     /pseudo
     CDS             3999..4163
                     /locus_tag="ACHKFK_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            4188..6587
                     /gene="dosP"
                     /locus_tag="ACHKFK_003679"
                     /gene_synonym="pdeO"
     CDS             4188..6587
                     /gene="dosP"
                     /locus_tag="ACHKFK_003679"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO9053927.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPQYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCINEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTSFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDEGKNRDYL
                     LSTAHNAMDFIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWYDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGIGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(6777..7214)
                     /gene="osmC"
                     /locus_tag="ACHKFK_003680"
     CDS             complement(6777..7214)
                     /gene="osmC"
                     /locus_tag="ACHKFK_003680"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO9053928.1"
                     /translation="MTIHKKGQAHWEGDIKCGKGTVSTESAVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSISIDTTADVSLDKVDAGFAITKIAL
                     KSEVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            7559..7774
                     /gene="bdm"
                     /locus_tag="ACHKFK_003681"
     CDS             7559..7774
                     /gene="bdm"
                     /locus_tag="ACHKFK_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO9053929.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            7876..8013
                     /gene="sra"
                     /locus_tag="ACHKFK_003682"
     CDS             7876..8013
                     /gene="sra"
                     /locus_tag="ACHKFK_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO9053930.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            8170..9867
                     /gene="maeA"
                     /locus_tag="ACHKFK_003683"
     CDS             8170..9867
                     /gene="maeA"
                     /locus_tag="ACHKFK_003683"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO9053931.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            10001..11011
                     /gene="adhP"
                     /locus_tag="ACHKFK_003684"
     CDS             10001..11011
                     /gene="adhP"
                     /locus_tag="ACHKFK_003684"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO9053932.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPSVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     ARIVQEKAGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            11197..11475
                     /locus_tag="ACHKFK_003685"
     CDS             11197..11475
                     /locus_tag="ACHKFK_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9053933.1"
                     /translation="MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLA
                     DIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVNGDAYILDYEDYH"
     gene            11475..11759
                     /gene="yddM"
                     /locus_tag="ACHKFK_003686"
     CDS             11475..11759
                     /gene="yddM"
                     /locus_tag="ACHKFK_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9053934.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(11988..12641)
                     /gene="fdnI"
                     /locus_tag="ACHKFK_003687"
     CDS             complement(11988..12641)
                     /gene="fdnI"
                     /locus_tag="ACHKFK_003687"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO9053935.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPIQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGL"
     gene            complement(12634..13518)
                     /gene="fdnH"
                     /locus_tag="ACHKFK_003688"
     CDS             complement(12634..13518)
                     /gene="fdnH"
                     /locus_tag="ACHKFK_003688"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO9053936.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEGNHE"
     gene            complement(13531..16578)
                     /gene="fdnG"
                     /locus_tag="ACHKFK_003689"
     CDS             complement(13531..16578)
                     /gene="fdnG"
                     /locus_tag="ACHKFK_003689"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15991..15993),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO9053937.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDDTLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGIANGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            16810..17691
                     /gene="yddG"
                     /locus_tag="ACHKFK_003690"
     CDS             16810..17691
                     /gene="yddG"
                     /locus_tag="ACHKFK_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO9053938.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDQIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYAHGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         4361 a         4539 c         4401 g         4417 t
ORIGIN      
        1 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
       61 tactatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa cgcttcctac
      121 caggttgctg cttatctggc ggatgaaatc gccaaactgg ggccgtatga gttcatctgt
      181 acgggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaagat
      241 ccgggataca ccctgtatga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg
      301 gccttcactc tcggcggtga agccactgac atcgtggtga tgcgcattat gtgtcgtcgc
      361 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
      421 ctcagcgacc acccgaaact gcagggtatt gcccagcaga acagctttaa acacacctga
      481 taacgtttaa cggtaacggt gtcccgaaac gaagccgttt cgggacaatt tccaaagtct
      541 gttcactggc attagcaacg gaaaatattg ttctgaatac gcttcagaac aaaacaggtg
      601 cggttccgac aggaataccg ttttaggggg ataatatggc tacatcagta cagacaggta
      661 aagctaagca gctcacatta ctcggatttt ttgccataac ggcatcgatg gtaatggctg
      721 tttatgaata ccctaccttc gcaacatcgg gcttttcatt agtcttcttc ctgctattag
      781 gcgggatttt atggtttatt cccgtgggac tttgtgctgc ggaaatggct accgtcgacg
      841 gctgggaaga aggtggcgtc ttcgcctggg tatcaaatac tctggggccg agatggggat
      901 ttgcagcgat ctcatttggc tatctgcaaa tcgccattgg ttttattccg atgctctatt
      961 tcgtgttagg ggcactctcc tacatcctga aatggccagc tctgaatgaa gaccccatta
     1021 ccaaaactat tgcagcactc atcattcttt gggcgctggc attaacgcag tttggaggca
     1081 cgaaatacac ggcgcgaatt gctaaagttg gcttcttcgc cggtatcctg ttacctgcat
     1141 ttattttgat cgcattagcg gctatttatc tgcactccgg tgcccccgtt gctatcgaaa
     1201 tggatgcgaa gaccttcttc cctgacttct ctaaagtggg caccctggtt gtatttgttg
     1261 ccttcatttt gagttatatg ggcgttgaag cttccgcaac ccacgtcaat gaaatgagca
     1321 accccgggcg cgactatcca ctggctatgt tactgctgat ggtggcggca atctgcttaa
     1381 gctctgttgg cggtttgtct attgcgatgg tcattccggg taatgaaatc aacctctccg
     1441 caggggtaat gcaaaccttt accgttctga tgtcacatgt ggcaccggaa attgagtgga
     1501 cggttcgcgt gatctccgca ctgctgttgc tgggtgttct ggcggaaatc gcttcctgga
     1561 ttgttggtcc ttctcgcggg atgtatgtca cagcgcagaa aaacctgctg ccagcggcat
     1621 tcgctaaaat gaacaaaaat ggcgtaccgg taacgctggt catttcgcag ctggtgatta
     1681 cttctatcgc gttgatcatc ctcaccaata ccggtggcgg taacaacatg tccttcctga
     1741 tcgcactggc gctgacggtg gtgatttatc tgtgtgctta tttcatgctg tttattggct
     1801 acattgtgtt ggttcttaaa catcctgact taaaacgcac atttaatatc cctggtggta
     1861 aaggggtgaa actggtcgtg gctattgtcg gtctgctgac ttcaattatg gcgtttattg
     1921 tttccttcct gccgccggat aacatccagg gtgactctac cgatatgtat gttgaattac
     1981 tggtcgttag tttcctggtg gtacttgccc tgccctttat tctctatgct gttcatgatc
     2041 gtaaaagcaa agcgaatacc ggcgtcactc tggagccaat caacagtcag aacgcaccaa
     2101 aaggtcactt cttcctgcac ccgcgtgcac gttcaccaca ctatattgtg atgaatgaca
     2161 agaaacacta accatgtctg aatggttata agacaaggga gcgataattc atcgctccct
     2221 ttttcgtgct tgttgtcgta ttgacttaac cgggataagt acgagaatga gcgcacatct
     2281 gtttaccgga aaccagcaca tatggatatc tgctcccgaa acgagaaatt agcgattaga
     2341 agaccagcga tactggttgc actggcactt ttactgtgta gttgtaaaag cacgcctcca
     2401 gagtccatgg tgacaccacc agcgggctca aagccaccag ccacgacgcc acaatcgtca
     2461 caaccgatgc gtggcatctg gctggccacg gtgtctcgcc tcgactggcc accggtttcc
     2521 tcggttaaca ttagtaaccc caccagccgg gcacgtgtac aacaacaggc gatgatcgac
     2581 aaactggatc atctgcaacg tctcggcata aacacggtct ttttccaggt caagccggat
     2641 ggtactgccc tgtggccatc gaaaattttg ccgtggtccg atcttatgac cggtaagatt
     2701 ggtgaaaatc cgggttacga tccgctgcaa tttatgctcg acgaagccca caagcgtggg
     2761 atgaaagtac acgcctggtt taacccctat cgcgtatcgg ttaatacgaa gcccggtact
     2821 atcagggaac tgaatagcac tctgtctcaa caaccggcga gcgtctatgt gcaacaccgc
     2881 gactggatca gaacgtccgg cgatcgcttt gtcctcgacc cgggcatccc tgaggttcag
     2941 gactggatca catcaatagt cgcggaagtg gtttcccgct atccagtaga tggcgtgcag
     3001 tttgatgact atttctatac tgagtcacca ggttcacggc taaatgataa cgaaacgtac
     3061 cgcaaatacg gcggcgcatt tgcgtcaaaa gcagactggc ggcgcaacaa tactcagcag
     3121 ttaattgcga aggtatcgca caccattaaa agcattaagc cggaagtcga atttggcgtt
     3181 agcccggcag gcgtgtggcg taaccgatca cacgatccgc tcggttccga tacccgaggc
     3241 gcggcagcct atgacgaatc ctacgctgat acccgtcgat gggtggaaca agggttgctg
     3301 gattacattg ctccccaaat ttactggcca ttctcacgga gtgccgcgcg ttatgacgtg
     3361 ttggcaaaat ggtgggcgga tgtcgttaaa ccgaccagga cccgcctgta tatcggtatc
     3421 gccttctata aagtgggtga accttcaaag atagagccag actggatgat taacggcggc
     3481 gtaccggaac tgaaaaagca gctcgatctt aacgatgctg tgccggaaat tagcggcaca
     3541 atcttgttcc gtgaggacta tctgaataaa ccgcagacgc aacaagcggt cagctatctg
     3601 caaagtcgtt ggggcagtta aactcccatt cttactttgt aacaagccgg tgctttaccc
     3661 actggcttgt taccctcttg aactatccca ctagcaatca tgcttaaata cgtcaaatta
     3721 accacgcaat taaccacact tagttaaaca gccataatcc ccctaataaa aatgtatgaa
     3781 tatgaaaaaa catacgatat tcgctgtcac cgaaaaataa ttacgatggg ctatactctt
     3841 ctgaagaaca gtagcgtaga ttgaaactgt cctaagtcgc ttatttcttt tcagtatatc
     3901 ttcatatttc aggagaatat ggtgcagtta gtcgatctcg cacgttgtgt ttggttgatt
     3961 ttggcctgcg gtttaaccat aaaggtcgac attattttaa agccgcaaac ggcgaggata
     4021 ttgccctctc actttccatc ggtgccgcca tgtttaatgg tcatcctgac tatgagcgcc
     4081 tcattcaaat agccgatgaa gctctgtata tcgccaaaag acgaggtaga aaccgtgttg
     4141 aactctgtaa agccagtctt tagatgcgcc aggatgcaga ggtaatcatg aagctaaccg
     4201 atgcggataa tgccgccgat ggcatttttt tccccgccct tgagcaaaat atgatgggcg
     4261 cggtgttaat taacgaaaat gatgaagtga tgtttttcaa ccccgccgca gagaagctct
     4321 gggggtacaa acgtgaagaa gtcattggca ataacattga tatgctgatt ccgcgggatt
     4381 tgcgtcctgc gcatcctcaa tacattcgtc acaaccgtga aggtggtaaa gcgcgcgtcg
     4441 aggggatgag tcgggagctg cagctggaga aaaaagacgg cagtaaaatc tggacccgtt
     4501 ttgcgctatc gaaagtgagc gccgagggga aagtttatta cctggcgctg gtacgggatg
     4561 ccagcgtaga aatggcgcaa aaagaacaga cccgacaatt gattattgcc gttgaccatc
     4621 tcgaccgacc ggtgattgtc ctcgatccgg aacgccatat tgtgcagtgc aatcgcgcat
     4681 ttaccgaaat gtttggttac tgcattaacg aagccagcgg tatgcagccc gatacactcc
     4741 tgaacattcc agaattccct gctgataacc gtattcgttt acaacagttg ctatggaaaa
     4801 ccgcccgcga tcaggacgaa tttctgctgt tgacgcgcac cggtgaaaaa atctggatta
     4861 aagcctctat cagcccggtt tatgacgtgc tcgcgcatct gcagaacctg gtaatgacat
     4921 tctcggatat cacagaagaa aggcaaatcc gccagcttga aggcaatatt ctcgccgcca
     4981 tgtgcagcag cccgccattt catgaaatgg gggaaatcat ttgtcgtaac atcgaatctg
     5041 ttctcaacga atcgcatgtt tcgctgttcg cgctgcgcaa cgggatgccg atacactggg
     5101 cgtcatcttc gcacggtgca gaagttcaaa atgcgcaaag ctggtcagcg accattcgtc
     5161 agcgcgatgg cgcgcctgcg gggatcctgc aaattaaaac ctcgtcagga gcagaaacca
     5221 gcgcctttat cgaacgcgtg gcagatatca gccagcatat ggccgcgctg gcgctggaac
     5281 aggaaaaaag ccgtcagcat attgaacaac tcatccaatt tgatccgatg accggtctgc
     5341 caaatcgcaa taacctgcac aattacctcg atgacctggt cgacaaagcc gtctctccgg
     5401 tggtgtatct catcggtgtt gaccatattc aggatgtgat tgatagcctt ggctatgcgt
     5461 gggccgatca ggcattgctg gaagtggtca atcgctttcg tgaaaaactc aaaccggatc
     5521 agtatctctg tcgtattgaa ggtacgtctt ttgtcctcgt gagcctggaa aacgatgtca
     5581 gtaacattac ccagatcgcc gatgagctac ggaatgtggt cagcaagccg ataatgattg
     5641 acgataaacc attcccgctc accttgagca ttggcatcag ctacgacgag ggtaaaaacc
     5701 gcgattactt gctctccact gctcacaatg caatggattt tattcgcaaa aatggcggta
     5761 acggctggca gttcttcagc ccggcgatga acgaaatggt aaaagagcgt ttggttttag
     5821 gcgcagcgct aaaagaagcg attagcaata accaacttaa actggtttat cagccgcaaa
     5881 tcttcgcaga aacgggtgaa ctgtacggca tcgaagccct tgctcgctgg tacgatcccc
     5941 tgcatggtca tgtgccccct tcacggttta ttcctctcgc agaagagatt ggtgaaatcg
     6001 aaaatattgg gcgctgggtc atcgcggaag cttgccgtca gttagcagaa tggcgtagcc
     6061 agaatattca tatcccggcg ttatccgtta acttgtctgc gctgcacttt cgcagtaatc
     6121 agctgcctaa tcaggtatct gatgcaatgc acgcctgggg tattgacggc caccagctga
     6181 cggtggaaat cacggaaagc atgatgatgg aacacgatac cgaaatcttt aagcgcattc
     6241 agatcctgcg cgatatgggc atcggtttat cggtagatga ttttggtaca ggcttttccg
     6301 gattatcccg cttagtcagt cttccggtaa cggaaatcaa aattgataaa agttttgtcg
     6361 atcgttgtct gaccgaaaaa cgcatccttg ccttgctcga agccattacc agcattgggc
     6421 aaagcctcaa tttaaccgtc gtggcggaag gcgtcgaaac caaagagcaa tttgagatgc
     6481 tacgcaagat ccactgtcgc gttattcagg gatatttctt ttcccgcccc ctgcccgccg
     6541 aagaaattcc aggttggatg agcagcgtgt taccgctgaa aatctgataa attcctctcg
     6601 cccgcactcg cgggttttta tttaacgtga cactgtcact taaatactgt gatttccgct
     6661 acgtttagtt ggacaacgac aacgcatagc catgtcagaa caatcccttc tcagcctgat
     6721 gtgattgtgc tgacgcatca ggcattcacg cgcctgacgc gtcatccggc aacgctttac
     6781 gatttcaact ggtaatccag cgtaatttcc gctttcagta cctgagaaac cgggcatcct
     6841 gcttttgctt tctggataat gccgtcaaag gtggaggcat caatccccgg caccgcaact
     6901 tcgctcttca gtgcgatttt agtaatcgca aaaccggcat ccactttatc cagcgagaca
     6961 tcggcggtgg tatcaatcga tatcgatgtt ggcgtgaatc ccgcttcccc cagcattaat
     7021 gaaagcgcca ttgagaaaca tgcggcatgc gctgcgccaa tcagttcttc agggttggtt
     7081 cctttttcgc cttcaaaacg cgtgttaaat ccatacggtt gttggttcag cacggcgctc
     7141 tcagtagata ctgttccctt cccgcatttg atatcgcctt cccagtgtgc ctgacctttc
     7201 ttatggattg tcattgttgc tctcctgtgg gcttaaaaag gaaattatag actacgtgag
     7261 aaacagcacg aggataagca ggataatccg aataaaatcc ggcgaaaata taattcctgc
     7321 acttaaatta aattcccata atagataacc ggaaatatat aactcaagag atattgcatc
     7381 aatatcaatc aactaatcac aatgataata cctatcaata tcatggagtt aaaatataaa
     7441 aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat gctgaatgtg
     7501 cgttataaat ctgcgatccg tagcagacac cataaataca cagacacgga gaatcactat
     7561 gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta atgccattga
     7621 gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca tggagctgac
     7681 caaatgggtg gaagcatctg ataacgacat cctcagtgat atctaccagc aaacgatcaa
     7741 ttatgtggtc agtggtcagc accctacgct ttaaggtgct atgcttgatc ggcaacctaa
     7801 tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa ctagcaacag
     7861 gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg actggaccat
     7921 aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt ggtaaataat
     7981 cccaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc tcaatgacga
     8041 ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata cttggtactt
     8101 gacgccctgg cggtaatgca aagacgataa aagcccccag ggatggatat tcaaaaaaga
     8161 gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc
     8221 cctgtattgc tggaatttcc gttgttgaat aaaggcagcg ccttcagcat ggaagaacgc
     8281 cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg
     8341 gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg
     8401 cgtaacatcc aggacactaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat
     8461 gagatgatgc cagtcattta caccccaacc gtcggcgcag cctgtgagcg tttttctgag
     8521 atctaccgcc gttcacgcgg tgtgtttatc tcttaccaga accgccacaa tatggacgat
     8581 attctgcaaa acgtaccaaa ccataatatc aaagtgattg tggtgactga cggcgaacgt
     8641 attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg
     8701 ctctataccg cctgtggcgg catcagtccg gcctataccc ttccggtggt gctggatgtc
     8761 ggcaccaaca accaacagct gcttaacgat ccgctgtata tgggctggcg taatccgcgt
     8821 atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc
     8881 tggccggacg tactgttgca gtttgaagac ttcgcacaaa aaaatgcgat gccgttactt
     8941 aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac cgcggcggta
     9001 acagtcggca cactgatcgc agccagccgc gcggcaggcg gtcagttaag cgagaaaaaa
     9061 atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag
     9121 acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat ggtcgatcgc
     9181 tttggcctgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa actggtgcag
     9241 aagcgcgaaa acctcagcga ctgggatacc gacagcgatg tgctgtcact gctggatgtg
     9301 gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg actgtttacg
     9361 gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct
     9421 aacccgacgt cacgcgtgga agccacaccg caggacatta tcgcctggac cgaaggtaac
     9481 gcgctggtcg ccactggcag tccatttaat ccagtggtat ggaaagataa aatctaccct
     9541 atcgcccagt gtaataacgc ctttattttc ccgggcatcg ggctgggtgt tattgcttcc
     9601 ggcgcgtcac gtatcaccga tgaaatgctg atgtcggcaa gtgaaacgct tgctcagtat
     9661 tcgccgctgg tcctgaacgg cgaaggtctg gtactgccgg aactgaaaga tattcagaaa
     9721 gtatcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtg
     9781 aaaacctccg ccgaagctct gcaacaggcc attgatgata atttctggca agccgaatac
     9841 cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct accgggcaat
     9901 ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc
     9961 ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg cagttgttac
    10021 gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc
    10081 cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga
    10141 ttttggtgac aaaaccggcg tgattctggg acacgaaggt attggtgtgg tggcagaagt
    10201 tggccctagt gttacttcat taaaaccagg cgatcgtgcc agcgtggcgt ggttctatga
    10261 aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa
    10321 aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg tcgccgatta
    10381 cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta cctgtgcggg
    10441 ggtcactacc tacaaagccg ttaagctgtc aaaaattcgt ccaggacagt ggattgctat
    10501 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg tctttaacgc
    10561 caaagtgatc gccattgatg tcaatgatga acagttaaaa ctggcaaccg aaatgggtgc
    10621 agatttagcg attaactcac gcaccgaaga cgccgccaga attgtgcagg agaaagccgg
    10681 tggcgctcac gcagcggtgg tgacagcggt agctaaagct gcgtttaact cggcggtgga
    10741 tgctgtccgt gcaggtggtc gtgtcgtggc tgtcggtctg ccgccggagt ctatgagcct
    10801 ggacatccca cggctggtgc tggatggtat tgaagtggtc ggttcgctgg tcggcacgcg
    10861 tcaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
    10921 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
    10981 tggccgtatg gtgattgatt tccgtcgcta agaggtcttt gctacgactg ccatgttcgg
    11041 gtcgcagcct gttttgcgag acgctttccg caatttcact cttcttcttt tcctccccca
    11101 tctttcccct cacaattcgc gaaaacgtag cgaaatttgt tgcgctgtac ggaacaatag
    11161 tgatagtgtt cctttcgagg gaacaaggat gaagagatga taatgaattt cagacacaag
    11221 ggattgcgtg acttgtttct tctcggcaaa acctccggcg ttataccgac gcaagtcaaa
    11281 cgattacgcc accgactcgc tgtgattgat gcagcatgct gtcttgctga tatcgatatg
    11341 cccggttacc gactacatcc gttaagcggc gatcgcgatg gaatttgggc gatatctgtc
    11401 tcgggcaact ggcgaatcac atttgaattc gtcaatggcg atgcatacat actggattac
    11461 gaggactatc actgatgaaa atggccaatc atccccgccc gggggacatt attcaggaat
    11521 cactggacga acttaatgtc agcctgcgcg agtttgccag agcaatggaa attgcgccct
    11581 caacggcaag ccgattgctg accggaaaag cagctttgac gccagaaatg gcaataaaac
    11641 tttccgtggt gatcggcagt tcgccgcaaa tgtggctgaa tctgcaaaat gcctggagtc
    11701 tggcagaggc agaaaaaaca gtggatgtgt cgcgactgcg ccgtctggtt acgcaataaa
    11761 aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat tgcccgatgc
    11821 gctacgctta tcaggcctac acgttcactg caatctattg gatttgaacg gtttgtaggc
    11881 cggataaggc gttcacgccg catccggcat gaacaaagtg tacgttgtct acaataaaaa
    11941 aatggcgcct tttacagcgc cattttcagc ttaaagctca aaagagatta taacccttct
    12001 tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg atggtgtttc
    12061 ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgatcc tttcacccag
    12121 aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc agccgcatgg
    12181 atcagcaggc tataacggac aacctgtatc ggaaagtact gcgcaaagta cggacgccag
    12241 ataatcactc cggtcaccag cagcacgaaa atcatgctca tgattgacca gaacatcatc
    12301 ttttgcccgg cgttgtactt accgacatcc gccactttgt gctcattgcc ttttaatact
    12361 tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg atgcacaaaa
    12421 cgcacaaaca taaacatcag cgcgacgaaa atcgcaatgc cgaagaacgg gtgcaaaatg
    12481 cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg gaagaagaac
    12541 gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg acaggcgcga
    12601 tcaataaatt tggtgcgcac aatcattttc gacttactca tgatttccct cctcgtcatc
    12661 gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg caaaagtggc
    12721 aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta ccgaggtatc
    12781 gatcttcgga tctttcggca gaccatgata cagctccggc tgatcggcat gatgcagcac
    12841 gtacataacg tgcgtaccac cgaccccttc cgggttgtag acgccagcat gttcgtaacc
    12901 acgcgctttc agtttcgcta cgcgctgttc cgccagctcc agcatctcct tcttggtgcc
    12961 gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc cgacgctgac
    13021 gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga ggcgcggaat
    13081 attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg actggaaatc
    13141 gacaatcccg ttagcgtact gaatgattgc accagcagac gggcatgcct tcaggcagcc
    13201 cggatcttca cagtgcatac agccatcttt acggatcagc cactccagct tgccgttctg
    13261 ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg cggggttatc
    13321 gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg aacacgccac
    13381 ctgacaggct ttacagccga tacaggtgga aacgtcgata agtttcgcga cttctgcttt
    13441 gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt tgataatgtc
    13501 ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttta ctaagaacgc
    13561 tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag tgttagcgat
    13621 ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca ccgtttcaac
    13681 ctgctgaccg tttacattca gcggtttcag acggcgcgtt accacagcca ccgcgcggat
    13741 aaagccacgc ttgctggaga cagtgacacg atcgccattg gcaatgcctt tcgccgccgc
    13801 cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga gcaatgcgtg
    13861 cttggtccag gtgtggaagt gctccgtcag acgataggtc gtacccacat acgggaactg
    13921 ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg ggttagacac
    13981 cacgttcgga tgcaacgggt tagtgcccag cggcgtttca attggctcgt agtgttccgg
    14041 gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt ccggctgcat
    14101 gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa tatcgttacc
    14161 cgtccacttg ctaccgttcc actggatcag catccgtttc ggatcccacg gtttaccgtt
    14221 gatatccgcc gaagcacggt tgtagagtac gcgacggttg agcggccacg cccaggccca
    14281 tcccagcgta ttccccagac cggacgggtc ggagttatcg cggttagcca tctggttgcc
    14341 ctgctctgtc cagctaccgg tgtagatcca gcaagacgat gcggttgtac cgtcatcacg
    14401 cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc cattggcatc
    14461 atagagatct tccagcgcat agccgttgtt ctctttagct acttcgtcag attgcggttc
    14521 gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt taccaccttc
    14581 ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc cgtcgttacg
    14641 cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac cggagttagc
    14701 gatagaacca tcttcttcag caaagcaggt cgaaggcaga cggaatactt cagtctgaat
    14761 agatgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag aggtttcagt
    14821 caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca ccactttgtt
    14881 tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aaataaccgg tgactttacc
    14941 ttcatccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact tcggcagcca
    15001 gtcgtagccc cagttgttct ctttctgcgc ggcatcgccg tagaaagatt tcatcaggct
    15061 aacgaaaaac ttcggatagt tgctccagta gttcacctga tcagccagcg tcgctttcgg
    15121 cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggca gcgtcaggta
    15181 acctggcagg ctggtagaga gcaggcctaa gtcagtcagg ccctgaatgt tggagtgacc
    15241 acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagta actggatcat
    15301 cgccatcgta cggatgttct gcgcacccac ggtgtgctgc gtccagccca gcgcgtacag
    15361 gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca ctttcaggaa
    15421 gtcggctttt ggcgtaccgc aaatgttttc tactacgtcc ggcgtgtagc gggaaacgtg
    15481 cgctttcagc agattccata cacagcgtgg atgagtcagc gtgtcatcgc gtttcgcata
    15541 gccgttttca tcgaactgat agttccagga cgacttatcg tattgacgtt tttcagcgtc
    15601 gtagccgctg aacagaccgt cttcgaaagc aaaatcatca cgcaccagca ggctggcgtt
    15661 ggtgtaatgc ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt agcgcaaaac
    15721 gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat ccgccacaga
    15781 agcggtacgc gtaaaacggg gatcgacaac gatcaaggtt gcatcgttgt tgtttttcgc
    15841 ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc ccatcaccat
    15901 tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc gaccaaatgt
    15961 tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt ctaccgccag
    16021 catcccgagg gagcgggcaa atttttgagt cagcatcccg gtttcgttgc tggcaccgga
    16081 agcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct cgttcttttc
    16141 aataaagtta gcgtcacggt cggctttcat cagcttcgca atacgggaga atgcttcttc
    16201 ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt agcgcagacg
    16261 gttttcgctg ttgacgtaat ccagcaaacc ggcccctttc gggcacagcg caccacggct
    16321 taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt ttttcgcgcc
    16381 atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc aggtgttacg
    16441 gatctctttc gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt gcttcggggc
    16501 aaagcccaat gctgctactg ttgttcccgc cataccgccc gcgcagattt taaaaaattg
    16561 tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg cgaggaaact
    16621 aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgtt tgccacaaaa
    16681 taatccgcct gcgcaaattg acctacctca atagcggtag aaaaacgccc cactgcctga
    16741 cagggcagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa gagagacaaa
    16801 acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat cgtcctgtgg
    16861 agcacgatgg taggattgat tcgcggtgtc agtgagggac tcggcccggt cggcggcgca
    16921 gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt tccgcgtatt
    16981 cgacagatcc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag ctatgaaatc
    17041 tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga agtggggatg
    17101 gtgaactatc tatggcccag cctgacaatt ctctttgcca ttctgtttaa tggtcagaaa
    17161 accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg ctgggtgtta
    17221 ggcggtgaca atgggttaca ttatgatcaa attattaata atatcaccac cagcccattg
    17281 agttatttcc tggcgttcat tggggcgttt atctgggcag cctactgcac agtaacgaat
    17341 aaatacgcac acggatttaa cggaattacc gtttttgtcc tgctaacggg agcaagtctg
    17401 tgggtttact attttcttac gccacaacca gaaatggtat ttagcacgcc cgtcatgatt
    17461 aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa tgtcggtata
    17521 ttacatggca acgtcaccat aatggcggta ggttcgtatt ttacgcccgt cctttcctca
    17581 gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca aggcgcgcta
    17641 atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggcta aaataattgt
    17701 cgggtctacg gcccggca
//
LOCUS       DAZGJT010000098        17204 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZGJT010000098 DAZGJT010000000
VERSION     DAZGJT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.098"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..1326
                     /gene="ybdN"
                     /locus_tag="ACHKFK_003691"
     CDS             106..1326
                     /gene="ybdN"
                     /locus_tag="ACHKFK_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO9053939.1"
                     /translation="MSIYKIPLPLNILEAAKEHITWTLNTLPRICVSFSGGKDSGLML
                     HLTAEIARQMGKKICVLFIDWEAQFSCTINYVQSLREFYADVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQQLCNPLYNIMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1299..1928
                     /gene="ybdM"
                     /locus_tag="ACHKFK_003692"
     CDS             1299..1928
                     /gene="ybdM"
                     /locus_tag="ACHKFK_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO9053940.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQFSRAWTVK"
     gene            complement(1929..3089)
                     /gene="ybdL"
                     /locus_tag="ACHKFK_003693"
     CDS             complement(1929..3089)
                     /gene="ybdL"
                     /locus_tag="ACHKFK_003693"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO9053941.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTRMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDVDSNITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRIALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQTDFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     IADMLHAEPEHYLALPEFYRQKRDILVNALSESRLEILACEGTYFLLVDYSAVSTLDD
                     VEFCQWLTREHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3198..4286
                     /gene="hcxA"
                     /locus_tag="ACHKFK_003694"
     CDS             3198..4286
                     /gene="hcxA"
                     /locus_tag="ACHKFK_003694"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO9053942.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGER
                     AIAAAQTKLPPAFELPGVKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQEYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQVEIDKMIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4296..4493)
                     /gene="ybdD"
                     /locus_tag="ACHKFK_003695"
     CDS             complement(4296..4493)
                     /gene="ybdD"
                     /locus_tag="ACHKFK_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9053943.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4606..6711)
                     /gene="cstA"
                     /locus_tag="ACHKFK_003696"
     CDS             complement(4606..6711)
                     /gene="cstA"
                     /locus_tag="ACHKFK_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO9053944.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWQLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6892..7305)
                     /gene="entH"
                     /locus_tag="ACHKFK_003697"
     CDS             complement(6892..7305)
                     /gene="entH"
                     /locus_tag="ACHKFK_003697"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO9053945.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7308..8054)
                     /gene="entA"
                     /locus_tag="ACHKFK_003698"
     CDS             complement(7308..8054)
                     /gene="entA"
                     /locus_tag="ACHKFK_003698"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO9053946.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRHQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8054..8911)
                     /gene="entB"
                     /locus_tag="ACHKFK_003699"
     CDS             complement(8054..8911)
                     /gene="entB"
                     /locus_tag="ACHKFK_003699"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO9053947.1"
                     /translation="MAIPKLQAYALPEPHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAEL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8925..10535)
                     /gene="entE"
                     /locus_tag="ACHKFK_003700"
     CDS             complement(8925..10535)
                     /gene="entE"
                     /locus_tag="ACHKFK_003700"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO9053948.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GEWQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLLNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRTSA"
     gene            complement(10545..11720)
                     /gene="entC"
                     /locus_tag="ACHKFK_003701"
     CDS             complement(10545..11720)
                     /gene="entC"
                     /locus_tag="ACHKFK_003701"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO9053949.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHLAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            11909..12865
                     /gene="fepB"
                     /locus_tag="ACHKFK_003702"
     CDS             11909..12865
                     /gene="fepB"
                     /locus_tag="ACHKFK_003702"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO9053950.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTALVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQIYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12869..14119)
                     /gene="entS"
                     /locus_tag="ACHKFK_003703"
     CDS             complement(12869..14119)
                     /gene="entS"
                     /locus_tag="ACHKFK_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO9053951.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMIGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSVRPGLLMLLSTLGAFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDN"
     gene            14230..15234
                     /gene="fepD"
                     /locus_tag="ACHKFK_003704"
     CDS             14230..15234
                     /gene="fepD"
                     /locus_tag="ACHKFK_003704"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO9053952.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLMMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLTAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLSTPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15231..16223
                     /gene="fepG"
                     /locus_tag="ACHKFK_003705"
     CDS             15231..16223
                     /gene="fepG"
                     /locus_tag="ACHKFK_003705"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO9053953.1"
                     /translation="MIYVSRRLIITCLLLLIACVMAGVWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVASHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16220..17035
                     /gene="fepC"
                     /locus_tag="ACHKFK_003706"
     CDS             16220..17035
                     /gene="fepC"
                     /locus_tag="ACHKFK_003706"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO9053954.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEDAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTTPSTAKI"
     gene            complement(17032..>17204)
                     /locus_tag="ACHKFK_003707"
     CDS             complement(17032..>17204)
                     /locus_tag="ACHKFK_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="in Salmonella is responsible for long O-antigen
                     polymerization; may be part of the ferric enterobactin
                     transport system; necessary for enetrobactin uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HFO9053955.1"
                     /translation="KYQLSPSLPVKKDGPGKAIIVILSALIGGMVACGGVLLRHAMAS
                     RKQDAIMADHLV"
BASE COUNT         4064 a         4923 c         4443 g         3774 t
ORIGIN      
        1 tcattgtgtg aatataaaat aatgtgctct atcttgcttc gtttatccta taattagcgg
       61 attcatctca cgaatcaccc cgtaacgttc agagagcgtt tttagatgtc tatttataaa
      121 atcccgcttc cactgaatat tctggaagct gcaaaagaac atattacctg gacacttaat
      181 accctacccc gtatctgtgt ttctttttcg ggtggtaaag attccggatt aatgctgcat
      241 ctgacagctg aaattgcccg acaaatgggc aaaaaaatct gcgttttgtt tatcgactgg
      301 gaagcgcagt tctcatgcac tattaactat gttcagtcat tacgtgagtt ttacgccgat
      361 gtcatcgaag agttttactg ggttgcgctc ccgcttacga cgcaaaattc cctttctcaa
      421 taccaacccg aatggcagtg ctgggaacct gatgtcgaat gggtgcgtca gcccccgcaa
      481 gatgcgataa ccgatcctga ctttttctcc ttttaccagc ctggcatgac cttcgaacaa
      541 tttgtgcgtg agtttgctga atggttttca caaaaacgtc cggcggcgat gatgatcggc
      601 atccgtgcag atgagtccta caaccgtttt gtcgccatcg ccagtttaaa taaacaacgt
      661 tttgccgatg ataagccctg gaccaccgcc gcaccaggcg gtcatagctg gtacatttac
      721 cccatctacg actggaaagt ggctgatatc tggacctggt atgctaacca tcaacagctc
      781 tgtaacccac tgtataatat aatgtatcag gcaggcgtcc ctctgcgtca tatgcgaatt
      841 tgcgaacctt ttggcccgga gcaacggcaa ggattatggc tctatcacgt tatcgaaccg
      901 gatcgctggg ctgctatgtg cgcacgagtc agcggggtaa aaagtggcgg catttacgcc
      961 ggacatgata atcattttta tggtcatcgg aaaatcctca agccagaaca tttagactgg
     1021 caagaatatg cattattgct gctcaatagc atgccggaaa aaacggctga gcattaccgc
     1081 aataaaatcg ctatttattt gcactggtat cagaaaaaag gcatcgaagt tccacaaacc
     1141 cagcaagggg acattggcgc gaaagatatc ccctcctggc ggcgaatatg caaagtttta
     1201 ctcaataacg attactggtg ccgggcatta tcatttagtc ctacgaaagc gaagaactat
     1261 cagcgttata acgaacggat aaaaggaaaa cgtcaggaat gggggatact atgcaacaac
     1321 gattaacgca ggatttaacc caatttctcg ccagcttgcc ggaagatgat cgcatcaaag
     1381 cgattaatga aatccgcatg gcgatccatc aggttagccc ctttcgtgaa gagccagtgg
     1441 attgcgttct gtgggttaaa aacagccaac ttatgcctaa tgattacaac ccaaacaacg
     1501 tggcaccacc cgaaaaaaaa ctgctgaaaa aatccattga aatagatggt tttacccaac
     1561 ccattgtggt cacgcatacg gataaaaatg ccctggaaat cgtggatggt tttcaccgcc
     1621 atgaaattgg taaaggctca tcatcgttaa aattacgctt aaaaggttat ttgccggtga
     1681 cctgtctgga aggtacgcgt aatcaacgca tcgccgcaac tattcgtcat aatcgcgctc
     1741 gtggacgtca ccaaatcact gccatgtcag aaatcgtccg agaactcagc cagttgggat
     1801 gggacgataa taaaataggc aaagagctgg gaatggacag cgacgaagtg ttacgcctga
     1861 agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca attttcccgc gcctggacgg
     1921 ttaaataact acagctggcg caggcgttct gctgctgcca gcaacgtcga ttccttcttg
     1981 gcaaaacaga gacgaatcag tttatgtggg aagggatcgg cgcaaaacac cgacagcgga
     2041 atcgccgcta cgccgtgctc ccgcgtcagc cactggcaaa actcaacatc atccagcgta
     2101 gatacggcgc tgtaatccac cagcaaaaag taagtacctt cgcacgctaa gatctccagc
     2161 cgactctcac ttaaggcatt caccagaata tcgcgcttct ggcgataaaa ttccggtaac
     2221 gcaagatagt gctcaggttc tgcatgtagc atatcggcaa tcgccagttg cgccggggta
     2281 ttcaccgaaa aggtcagata ctgatgcact ttgcggatct cggcgctgat gggcgcaggt
     2341 gcaacgcaat agcccacttt ccagccggtc atatgatagg tcttgccaaa cgatgacacc
     2401 gccaccgctc gctcacgcaa ttgcggatgc gccagcacgc tggcatggcc ctgttgtgaa
     2461 aaattgatgt gctcgtagac ttcatcgcta atgacaaaaa tctcgtgccc ggcgatcgcc
     2521 tgccacagag cggcgaaatc agtctgctgc cagacagttg cactggggtt atgcggggtg
     2581 ttgaggatca ccagtcgggt gcgctcgctt aacaatgcgg caaattcctg ccagtcaacg
     2641 cgaaaatgcg gcggttgcag tgcaatacgt ttcactattc cccccgaaag tgcgatggcg
     2701 ggggcgtaac tgtcatagct gggatcaaaa caaatcactt cgtcgccatt gcgcaccaat
     2761 gcggttatcg ccgcgtataa cgcctccgtc gcccctgccg tcacggtgat attgctgtcg
     2821 acatccggtt gatagccata cagacgttcc gttttctgag caatcgcctc gcgcaaggcc
     2881 tgcacgccgg tcatgggcgc gtattggttt gccccctggg caacgtggta cgccagccgc
     2941 tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt gcgaaaggtt aatcgcctgg
     3001 tgttgctgcg ccagcgcgct catccgggtg aaaatagtgg tgccaagttg tggaagtttg
     3061 ctttgtggaa tcagagggtt atttgtcatt tttattgtac ctgacgaagt tgcgtgttga
     3121 aggcactata acacgatgct agtatttggc aatcaagacg tttagatgtc taaataaaac
     3181 aataaggaca acacaacatg cctcacaatc ctatccgcgt ggtcgtcggc ccggctaact
     3241 acttttcaca tcccggaagt ttcaatcacc tgcacgattt tttcactgat gaacaacttt
     3301 ctcgcgcggt gtggatctac ggcgaacgcg ccattgctgc ggcacaaacc aaactgccgc
     3361 ctgcgtttga actgccaggg gtaaagcata ttttgtttcg tggtcattgc agcgaaagcg
     3421 atgtgcaaca actggcggcg gagtccggtg acgatcgcag cgtggtgatt ggcgtcggcg
     3481 gcggcgcact actcgacacc gcgaaagccc tcgcccgccg tctcggtctg ccgtttgttg
     3541 ccgttccgac gatcgccgct acctgcgccg cctggacacc gctctccgtc tggtataacg
     3601 atgccggaca agcgctgcat tatgagattt tcgacgacgc caattttatg gtgctggtgg
     3661 aaccggagat tatcctcaat gcgccgcaag aatatctgct ggcggggatc ggtgacacgc
     3721 tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc agaaacgttg ccgctaaccg
     3781 tgcggctggg aatcaataat gcgcaggcca ttcgcgacgt cttgttaaac agtagcgaac
     3841 aggcgcttgc cgatcagcaa aatcagcaat taacgcaatc attttgcgat gtggtggatg
     3901 ccattattgc tggtggtggg atggtcggtg gtctgggcga tcgttttacg cgtgtggcgg
     3961 cggcccatgc cgtgcataac ggtctgaccg ttttgccaca aaccgagaag tttctccacg
     4021 gcaccaaagt cgcctacgga attctggtgc aaagcgcctt gctgggtcag gatgatgtgc
     4081 tggcgcaatt aactggggcg tatcagcgtt ttcatctgcc gactacgctg gcggagctgg
     4141 aagtggatat caataatcag gtggagatcg acaaaatgat tgcccacacc ctacgtccgg
     4201 tggaatccat tcattacctg ccagtcacgc tgacaccaga taccttgcgt gcagcgttcg
     4261 aaaaagtgga atcgtttaaa gcctgacgta caaatttagc agcagcgcgc gccgcctttt
     4321 ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct cgtaggtcat cggcgtttga
     4381 tcgggatggt taacccgcat atgttcgaca tagttgtcgt aatcaggcat accaatcatc
     4441 agtttcgccg cctgacctaa atattttccg gcttttgcca gtgaatcaaa catctaaacc
     4501 tccggtaata caaatgccct cctgaattaa gagggcttaa ttgctatcaa cacgctcggg
     4561 ctgagagtaa ggggagcgct cagcacattc cccctcactc tgactttagt gcgcgccttt
     4621 cgcctgcgcc acgatctcct cgacattttc cggcatcggc tcatatggcg tttctttcgc
     4681 cgttggcttc ggctctttca atgccgccag tgccgtctta atcgagaaca gcgccagaac
     4741 caccacaacc accataaaga agatggttaa tccggcatcc agacggttgt tgaacaccag
     4801 ttgcgccagt tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg
     4861 gaacttgtta gcaatcgcca ggaatccgac tttcgcatcc gggctaaacg ctttctgcca
     4921 gcctgcggtc agggtacaaa tcagcagcca ggccgttggt accagcgcca cccaggcgta
     4981 acgttgacgt ttcattttga acagcaccac agcacagagc atcagcgcca tccctgccag
     5041 catctggttg gcaataccaa acagctgcca caaggtgtta ataccgccca gcggatcgac
     5101 cacgccctga tgcaggaagt agccccacgc cagcacgcac agcgccgttg ccagcaggtt
     5161 agcaggcagt gaatcggtcc gtttcaggcc cggagacacc acgcccagca gatcctgcaa
     5221 cataaagcgc gcagcacgcg tacccgcatc caccgccgta agaataaaca gtgcttcaaa
     5281 cagaatggcg aagtgatacc agaacgccac atccatcatg ccgcccagcg caccgtgcag
     5341 aatgtaggcc atccccaccg ccagggtcgg cgcaccgccc gcacgggaaa tgatcgactg
     5401 ttcacccact tcgctggcaa tctggtttag cgtatccggg gtaatcgcaa agccccagct
     5461 actcaccacc tgcgcggcag aagcaaccac atccgccgtc cctgccggag ccagcacagc
     5521 catcgggctg ttcatggcga agtacacacc cggatcgatg atacaggcgg aaaccagcgc
     5581 cataatcgcc acgaaggatt ccattaacat cccaccgtaa ccgataaagc acgcctgccc
     5641 ttcgttcgcc agcatcttcg gtgtagtgcc ggaagagatc agtgcatgga agccagacac
     5701 cgcgccacag gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc
     5761 agtgccatcg acaaatttag ttagcgcagg catggtcagc gtcgggcgca taatcaaaat
     5821 gcccaccgcc agaccgacga tagtcccgat tttcaggaag gttgagaggt agtcacgcgg
     5881 agccagcagc aaccacaccg gcagcaccgc cgccacaaaa ccgtaaccca ccagcatcca
     5941 ggtcagctgc acgccggtaa agtcaaagta cggtgcccac gtcgggcttt ctgccaccca
     6001 gccgccagag ataatggcga aaataaggaa caccagacca atcaccgaca cttcgccaat
     6061 acgccccgga cgcaaatagc gcaggtagat ccccataaac agcgccagcg gaatggtgaa
     6121 cgcaacggta tacgttcccc acgggctatg agtcagggct ttaaccacga tcatcgccag
     6181 cacggcaagg ataatgacca tgatcataaa gcaggccacc agcgctatca ccccggcggt
     6241 tggccccatc tcttctttga ccagctcacc cagcgagcga ccgtcacggc gcgtggaaac
     6301 aaacagcacc atgaaatcct gcaccgcacc ggcgagaacc acccctgcga gcagccagat
     6361 catccctggc aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc
     6421 ggcaatggcc gcaaaatggt gaccgaacag cacttttttg tccgtcggca catagtccag
     6481 cccgtcgtta tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt
     6541 ggcgatatac agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac
     6601 aatccacagc gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc
     6661 tcccattaca gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat
     6721 ccgtgtgcgt gtccatagag atgttacatt ttgtttctat aacatctttg tacaatttaa
     6781 caacacagca accatgcaaa tcggagttga aaccaatatt taacttaaca atgttaactc
     6841 gatcactccg ttaaccgaat tttcctgcaa tctcatttta ttctgtcggg ttcatcccaa
     6901 aaccgctgtg cccagccgac aggtgcagca acgccgcccc tgttcatcga aaacgacgat
     6961 ttcccagctc tgattttgtc gtccaagatg cagcggctgg cagacaccgc gcacctttcc
     7021 ctcagacacc gggcgatgat gtgtggcgtt aagttccgtg cccaccacac actgtccgtc
     7081 gcgggtcatc ataaatccgg ccatcgatcc cagcgtttcc gccagcgccg ccgacgcgcc
     7141 gccatgcagc aggccaaacg gctgatgagt acgggtatca accggcattt cggcttcgag
     7201 tacatcatcg cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct
     7261 ggtggcgttc agctcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc
     7321 gttgagccgc catcgaccac aatatcctgt agggtaatat ggctggcgag gtcagaggcg
     7381 aggaacaaaa tcgtgttggc gatctcctgc ggacgggcga ttttccctag cggaatgccg
     7441 agtttaaact gctcgccaaa gccgcgaata cgctgctctt cggcgtcatc gctcacccac
     7501 agcgtgcgtt gcatatcagt gtcggtggag cccggcgaaa ccacattaca gcgcacgccg
     7561 ctacccgcca gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg
     7621 ccataagcac tcatgccaat gcgcggcgtg tgtgcggcgt cggacgcaac agtaacaatc
     7681 gccccgcccc gctgatggcg aaactggttc atggtctgct gaaacaggtt aaacgcaccg
     7741 ccgacgttga ccgcaaaagt ctgctgccag tcctctttac tgagctgatc ggtcgcaccc
     7801 atgcgcaaaa ttcccgccgc attgaccagc acgtccagtc gctccgtttc agctaacagt
     7861 cgctgacata cctgcgccac ctgcgcagca tcggcaacat ccattacttc ggtcgcaaaa
     7921 ggatattgct cctgagtaaa cgcttgatca aaacctgtaa ctttcgcccc cgcctcaaca
     7981 aatgccagcg ccgtggcgta gcctatacct ttccccgcgc cagttaccca gacattttta
     8041 ccgctgaaat ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg
     8101 ggtttttcgc cagcatgaca aagtcgatat cgccatgcac tttgcgccag cgcgccgcca
     8161 gcgccatcat gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaagggtt
     8221 catcggactc gtccagcaac ggcaggatca cctcacgcag ctccgctttg ctggcgggga
     8281 taggtgctgg cagcaattct tcggtcatca ccacccgacc agaacgtccg gccacatatt
     8341 tcagcgacat caaatgctcg tcacggctga aatcggccag cgcatccgcc accataaacg
     8401 gtttaatatc gcgcataaat gcgtccgttg cggtggtcat acagccaatg tgggcatata
     8461 ccccggtaat aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac
     8521 gatgaaatgc gctgtagcgc catttcacca gcaccgtgtc gtcggcatct ggcgtcaggc
     8581 gatccaccac cttttgctgt tccggcgaac gggtcaggcc aggcccccac atatcattca
     8641 acagcgcccg atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accgggatat
     8701 tgtgctgttt gcagtagtcg cgtagtgccg caatattcgc gatcacctgc tccatcatcg
     8761 ggcagttctc accccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg
     8821 cacgttgcgg ttcaaaggcc cagtcgactt tattctgcgg aatatcatga ggctccggca
     8881 gtgcgtaagc ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgtgcgt
     8941 gacgccagcc actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt
     9001 gaatctacac actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg
     9061 aaacgacgca cctgcaccgc gcgcagcggc tcttttacca ccagataagc acagcttttt
     9121 tcgcccatca gctcatcttc catgctcacc agtgcggcgt agatcaccgc cggatggcgc
     9181 agcagcaggt tttcgatctc ttcggcagcg atcttctcgc cgccacggtt gatctgatct
     9241 ttctcgcgcc cctgcacagt gatgtaaccc tctggatcaa tagagatcag atcgccggaa
     9301 cagtaaaaac cgttggcatc aaaggcgctg gcattgtgct gcgggctttt gtaatagcca
     9361 cggaaggtgt acggcccgcg cgtcatcagg cgtccaactt ccccttgcgg cagtggattt
     9421 ccttcggcat cggcaaccca tacttcatcg tccgggcaca ttgggtaacc ctgggtatgg
     9481 ataattttct ccgcgctatc atcaagtcga gtgtagttca ccagcccttc cgccatgcca
     9541 aacacctgct gcaactggca gccaatctca gcgggaatac gcgccgcaag ggtggcagaa
     9601 agacgtgcgc cgccgacctg caacagtttc agcgaggcaa gctgcgcccg gctttcacct
     9661 tcggtcagcg cctgcaacca caggctgact gccggtggca ccagcgcggt gacgttcacc
     9721 tgatgttttt caatcaatgg gaagcaaagc gtggcgctag gatcggcagc cagaacgaca
     9781 gtgccgccag cgagaaatac ccccaacgat cccggtgaac tcatggcgta gttatgagcc
     9841 gccgggatcg cgcacaggta gcgcgtctgt tgtgtgaact gacaaatctc gacgctacga
     9901 cgcacgctgt agtagtagtc gttatgagtg cgcgggatca gtttcggagt gccggtggtg
     9961 ccgccggaaa gctggaaata ggccacttca tcagcaggag atggcgtggc agtaaaatcc
    10021 tcagccggat ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gagcagctgc
    10081 accacgcgaa tggaagaatg ctctgcaaca aatgtgttga ggaaatcatc cccgctaaat
    10141 agcgcatgtt ggcgatcggc aatcagtaac gccggttcaa tctggctggc ataggcgttc
    10201 agttcactac gctgatggct gaacagcgcc agcaccggcg caacgcccag tttcagcagc
    10261 gcgaaaaagg taatgtaaag ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca
    10321 ggtttaatgc cctgacggcg caaactacag gcgaggttat ccgccgcctg attcagctcc
    10381 cggtaactca actgccactc gccgtcgatc accgcgatgc tgtcactcgc agcgtggcga
    10441 gtcagaatgt cggtcagtgg caaatcctgc cagtagcctt tttcccgata gcgacgggca
    10501 aactcttccg gccatcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa
    10561 acgttcaaca tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgaggac
    10621 gcaggcacaa tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag
    10681 cggatggtca ccacccattc accgttacct tcgctgtcac accaacccac aatgccgcca
    10741 aacagttcgc ggtcgaacgg ctccagttca gcaataacct gagtcgcggc cagatgcggg
    10801 aaaccgctca gcgcaggagt tggatgcagc agacaggcca gagtcagtgc gttttcttgc
    10861 gaattcgctt taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat
    10921 tgtggagagg agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc
    10981 tgagtcacca gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct
    11041 tcgcgatcga gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta
    11101 aaacgctcgc cgtctttacg tagcagcagt tccgggctgg cccccagcag aacgccacca
    11161 tcagccaacg gaacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc
    11221 aatacgccac tatcaatggc ggcgtcagtg gtgatatcaa tcaaccgtga caacaccact
    11281 ttgtcgacct gcggcgtggc ggtaagtgcg gcagcacggg caaccatctg ttcaaacgtg
    11341 gtttgttccg gaatggcctg acgttccacc acgttcagcg actggctgcg agtgaaacgg
    11401 cgtgcggaag cttgtttttc ctgacgggag aacgactgcc aggattcggg tatatacagc
    11461 gacgaaggct gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc
    11521 tgcgctttgg catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa
    11581 tccccgttca cagccggttc atcgaagcgg gcgaaacatc ctgacgtcgt aaaactgcgg
    11641 tacggcgaca taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc
    11701 tcagccagtg acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta
    11761 tcatttatat tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt
    11821 aacgcaatct gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt
    11881 aacttcatta ataacaggaa gctgacttgt gagactcgcc ccgctctacc gcaacgccct
    11941 tctattaaca ggacttttgc tttcaggaat agccgcagtt caggccgccg actggccgcg
    12001 tcagattact gacagccgtg gcactcatac cctggaaagc cagccgcagc gtattgtttc
    12061 caccagcgtc accctgaccg gctcactgct ggcgattgat gctccggtga tcgccagcgg
    12121 cgcgaccacg ccaaataacc gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa
    12181 ggtggcgaaa gaacgcaaac tgcaacggct ctatatcggc gaaccgagtg cagaagccgt
    12241 tgccgcgcaa atgccggatc tgattttaat tagcgcaacc ggcggcgatt cggcgctggc
    12301 gctgtacgat cagctttcta ccatcgcccc aacattaatc atcaattacg acgacaaaag
    12361 ctggcagtcg ctgttaacgc agcttgggga aattaccggg catgagaaac aagcggcaga
    12421 gcggattgca cagtttgata agcaactggc agcggcgaaa gagcaaatca aattaccgcc
    12481 gcagccggtc actgcccttg tctataccgc cgctgcacac agtgccaatc tctggacgcc
    12541 ggaatcagca caagggcaga tgctggaaca actcggcttt acgctggcga agttgcccgc
    12601 aggcttaaac gccagccaaa gccagggcaa acgccatgac atcattcagc ttggtggaga
    12661 aaatctggct gcagggttaa atggcgagtc actattcctg ttcgctggtg atcagaaaga
    12721 cgccgatgct atttatgcta atccgctgct cgcgcacctg cctgcagtac aaaacaagca
    12781 gatttatgcg ctgggaaccg agacattccg cctggattac tacagcgcca tgcaagtgct
    12841 ggaacgactt aaggcgctgt tttaagcatt aattgtcgga cgctgtcacc tgcggcggcg
    12901 tctggcgaaa acgccgcaac tccaccagca ccagcaacaa caacacgccg ataatcaaca
    12961 aaccaaaacc gctcgcactt gcggaagcaa ccggtgtcat catcgctccc aaaccgccca
    13021 gcagcgccgc gcctatggca tcgcccgtca cgttttgcgc cgtccacaaa ccgttaatcc
    13081 gccctaacat cgcttccggg gtttgcgttt gcagcattgt atattgcagc aacgaactaa
    13141 ccgcgctcag ccagccgaac agtgccagac aaaccacgcc taaaatccac atcggcatca
    13201 ggccaaacag accaatggcg aggaacgccc ccaatgtgga gagcaacatc aacagacctg
    13261 gccgtacgct atgcgccagt tttccactgg ttaacgcgcc aatagccgca ccgagcggga
    13321 ttgccgcata gagaaagcca atttgcgccg ccgacatctg ccagttgtca gccagcgccg
    13381 gatacaggac ccgcaccgcg ctcgccatcg tcaataaacc acccagcaac gcaatccctc
    13441 ccaccagcgg gctggcgagt agaaaacgaa atcctgccag taatgatttc aacggatgct
    13501 cacgcggctg cggcggtggt ggcaacgccg gaaggcttaa caacggtagc aaggtaataa
    13561 acgtgcccgc cgccgccagc ccgtagttcc aggctacgcc tccggtcgcc agcagtaaac
    13621 caccaatcat gggcgaaatc accgacccca gacgcacagt caacatggtg atcgccccag
    13681 cctgcattag gttttcacgt cctaccagtg caggtgtcgc cgccagtagc gccgtaacgc
    13741 caagtgatgc gaaaaaacca tcccataaac caagtaaata gattgccagc aaagacggct
    13801 ccggcagcag cgcattcagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca
    13861 gcaaaatcac ttttttgcgc tcatagcgat ccgccagcac accaccgatc atcagaccga
    13921 caaacatagc accgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgtgtca
    13981 tcatctggat ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa
    14041 aacgagcgag gaatactgcg cgaaacgccg ggtgcgtttt taacaggctg aggttaagca
    14101 gccaggattg tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta
    14161 ggctgcacat gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg
    14221 gaagttcgta tgtctggttc tgttgccgtg acacgcgcta ttgccgtgcc cggattgctg
    14281 ttattactga ttatcgcgac ggcattaagc ctgctcattg gagcaaaatc actccccgct
    14341 tccgtagtgc tggaagccct ctccggcacc tgccagagcg ccgactgcac catcgtgctc
    14401 gacgcacgac tgccgcgcac ccttgccggt ttactggcag gcggtgcgct tggccttgcc
    14461 ggggcgttaa tgcaaaccct cacccgaaac ccacttgccg accccggctt acttggcgtg
    14521 aatgccggag ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcttccgcg
    14581 caggaacaac tgatgatggc cttcgccggc gcgctggtgg cgtcattgat tgttgccttt
    14641 accggcagcc agggcggcgg gcagttaagt ccggtgcgtt taaccctggc aggcgtggcg
    14701 ctggcggcgg tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgacgtctac
    14761 gatcaactgc gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa
    14821 gtggtgctga tcccggtgct taccgccgga gcaaccgcgc tattactgag ccgcgcgctg
    14881 aacagtttaa gccttggcag tgacaccgcg acggcgctgg gcagtcgcgt agcgcgcaca
    14941 cagttgattg gtctgctggc aattaccgtg ctttgtggta gtgcgacggc ggtagttggc
    15001 ccgattgcct ttattggcct gatgatgccg cacatggcgc gctggctggt aggtgccgat
    15061 catcgctggt cgctgcccgt cacgctactc tctacccctg ccctgctgct gtttgccgat
    15121 atcattgggc gggtgattgt tcccggcgaa ctgcgcgttt ctgtagtcag tgcctttatt
    15181 ggtgcaccag tgctgatctt cctcgtacga cgtaaaacgc gaggtggcgc atgatttacg
    15241 tctctcgccg attaattatc acctgtctac tgttgctaat agcttgtgta atggcaggcg
    15301 tgtggggatt acgcagcggt gccgtcacgc tggaaacctc gcaggtattc gccgcgctga
    15361 tgggcgatgc gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc
    15421 tgatggcgct gttgattggc gcggcgctgg gcgtcagcgg cgcgattttt cagtcgctga
    15481 tgcgtaaccc gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg
    15541 tgctggtggc gatggtgctg tttggtcagg acctgacggc tatcgccctg gcagcaatgg
    15601 tgggcggcat tgtcacttcg ctgttggtct ggctgctcgc ctggcgtaac ggtatcgaca
    15661 cctttcggtt gattattatc ggtatcggcg ttcgcgccat gctggtggcc tttaatacct
    15721 ggctgttgct gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgccggat
    15781 cgctcaatgg cctgacgtgg gcaaaaacct caccttccgc acccatcatt atattaatgt
    15841 taattgccgc tgccttgctg gtgcggcgaa tgcgcttgct ggaaatgggc gatgataccg
    15901 cgtgtgcgct gggcgtcagc gtcgaacgtt cgcggctgtt aatgatgctg gtcgccgtag
    15961 tgcttaccgc agctgccact gccctggcgg ggccaatttc ctttattgct ttagtcgcgt
    16021 cacacattgc ccgccgcatc agtggcaccg ctcgctgggg gctgacccag gcggcgctgt
    16081 gcggggcact tttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc
    16141 aacttccggt tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa
    16201 ttcaggagtc tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgtggcg aacagttaac
    16261 cctcggatat ggcaaatata ccgttgcgga aaatctgact gtagaaatac ctgatggtca
    16321 cttcacggca attatcgggc caaatggctg cggtaaatcc acgttactgc gtaccttaag
    16381 ccgcctgatg acgcctgctc acgggcatgt ctggctggat ggcgagcaca ttcaacatta
    16441 cgccagtaaa gaggttgcac gccggattgg tctgttggcg caaaatgcta ccacaccggg
    16501 cgatatcacc gtgcaggagc tggtggcgcg tgggcgttac ccgcatcaac cgctgtttac
    16561 ccgctggcgc aaagaagatg aagacgcggt aacaaaagcg atgcaggcca cgggaataac
    16621 tcatctggcg gatcaaagcg tggacaccct ttctggcgga caacgccagc gagcgtggat
    16681 cgcgatggtg ctggcccagg aaacggcaat tatgctgctc gacgaaccca cgacctggct
    16741 ggatatcagt catcagattg atttgctgga actgttaagc gaactgaacc gcgagaaagg
    16801 ctataccctg gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt
    16861 gattgcattg cgggaaggaa aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc
    16921 tgaactgatc gagcgcattt atggtctgcg ctgcatgatt attgacgatc cggttgccgg
    16981 aacgccgctc gtggtgccgc tcggacgaac gacaccttca accgcgaaga tttaaactaa
    17041 gtggtctgcc attatggcat cctgttttct ggatgccatc gcatgacgca acagcacacc
    17101 accacaagcc accatcccgc cgatcaacgc ggaaaggatc acgataatag ccttacccgg
    17161 accgtctttt ttcactggca atgacgggct taactgatat ttaa
//
LOCUS       DAZGJT010000099        16209 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZGJT010000099 DAZGJT010000000
VERSION     DAZGJT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16209
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.099"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(125..442)
                     /locus_tag="ACHKFK_003708"
                     /pseudo
     CDS             complement(125..442)
                     /locus_tag="ACHKFK_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535034.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
     gene            complement(597..672)
                     /locus_tag="ACHKFK_003709"
     tRNA            complement(597..672)
                     /locus_tag="ACHKFK_003709"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(637..639),aa:Thr,seq:cgt)
     gene            complement(787..2040)
                     /gene="proA"
                     /locus_tag="ACHKFK_003710"
     CDS             complement(787..2040)
                     /gene="proA"
                     /locus_tag="ACHKFK_003710"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO9053956.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSESILN
                     ANAQDVADACANGLSEAMLDRLALTPARLKGIADDVRQVCNLADLVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(2052..3155)
                     /gene="proB"
                     /locus_tag="ACHKFK_003711"
     CDS             complement(2052..3155)
                     /gene="proB"
                     /locus_tag="ACHKFK_003711"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO9053957.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            3444..4499
                     /gene="phoE"
                     /locus_tag="ACHKFK_003712"
     CDS             3444..4499
                     /gene="phoE"
                     /locus_tag="ACHKFK_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO9053958.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASSLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPISGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYVDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(4538..4939)
                     /gene="crl"
                     /locus_tag="ACHKFK_003713"
     CDS             complement(4538..4939)
                     /gene="crl"
                     /locus_tag="ACHKFK_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9053959.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWTSVQIKDAKVVERLEHTLRE
                     FHEKLRELLATLNLKLEPADDFRDEPVKLTA"
     gene            complement(4997..6241)
                     /gene="frsA"
                     /locus_tag="ACHKFK_003714"
     CDS             complement(4997..6241)
                     /gene="frsA"
                     /locus_tag="ACHKFK_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9053960.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDGLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            complement(6333..6791)
                     /gene="gpt"
                     /locus_tag="ACHKFK_003715"
     CDS             complement(6333..6791)
                     /gene="gpt"
                     /locus_tag="ACHKFK_003715"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9053961.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            7052..8509
                     /gene="pepD"
                     /locus_tag="ACHKFK_003716"
     CDS             7052..8509
                     /gene="pepD"
                     /locus_tag="ACHKFK_003716"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9053962.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(8566..9180)
                     /gene="prfH"
                     /locus_tag="ACHKFK_003717"
     CDS             complement(8566..9180)
                     /gene="prfH"
                     /locus_tag="ACHKFK_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9053963.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(9177..10316)
                     /locus_tag="ACHKFK_003718"
     CDS             complement(9177..10316)
                     /locus_tag="ACHKFK_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO9053964.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESSAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(10507..10959)
                     /gene="yafP"
                     /locus_tag="ACHKFK_003719"
     CDS             complement(10507..10959)
                     /gene="yafP"
                     /locus_tag="ACHKFK_003719"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9053965.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            complement(10969..11367)
                     /gene="yafO"
                     /locus_tag="ACHKFK_003720"
     CDS             complement(10969..11367)
                     /gene="yafO"
                     /locus_tag="ACHKFK_003720"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO9053966.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            complement(11370..11663)
                     /gene="yafN"
                     /locus_tag="ACHKFK_003721"
     CDS             complement(11370..11663)
                     /gene="yafN"
                     /locus_tag="ACHKFK_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO9053967.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            complement(11715..12770)
                     /gene="dinB"
                     /locus_tag="ACHKFK_003722"
     CDS             complement(11715..12770)
                     /gene="dinB"
                     /locus_tag="ACHKFK_003722"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO9053968.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERAVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(12841..13626)
                     /gene="lafU"
                     /locus_tag="ACHKFK_003723"
     CDS             complement(12841..13626)
                     /gene="lafU"
                     /locus_tag="ACHKFK_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO9053969.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     LPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            13598..15310
                     /locus_tag="ACHKFK_003724"
                     /pseudo
     CDS             13598..15310
                     /locus_tag="ACHKFK_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(15530..16027)
                     /gene="rayT"
                     /locus_tag="ACHKFK_003725"
     CDS             complement(15530..16027)
                     /gene="rayT"
                     /locus_tag="ACHKFK_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HFO9053970.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLQNRRNQLLTTQFQTLRNVIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVAKGLYPIDWAGDVTDFSAG
                     ERIIL"
BASE COUNT         3979 a         4226 c         4015 g         3989 t
ORIGIN      
        1 tgtccaggag tttaaggaca aatctcatac tgattatcgg aagcttttat cattatatga
       61 ttaccaagca tacaatgtat aattatatac actattattg tgattataat ttgttactgt
      121 gtcactcaag ggcaatatct ttgaaggcgt tgtttaactg cgtttcccgg gcaatctttt
      181 cctctcgctt cacttccatc agatcgattc ccccagagat acctctttta gcttcttcaa
      241 gttttgcagg agcatcagct aaggtgacct caggatacac acctagtgct aacagcttct
      301 ctttaccagc tacacgatac ttgagacgcc aatattctcc tccaccaggt tttaccagga
      361 ggtacagacc accaccatca gccaacttgt aagccttctc ttttggcttg gcagtgtcta
      421 tttgacgggc attgagtttc acttgggggt atctccacta aactgaacag caaatcccct
      481 caattgcttg tagagtttgg ggtacttaaa tagacgtcaa aagactaaaa ggggcattaa
      541 tatactgatt ataagaggtt tttaaatact tgagtagact tggggagacg ttagaatggt
      601 gccgataaga ggagtcgaac ctccgacctt cgcattacga atgcgctgct ctaccaactg
      661 agctatatcg gccctgaaag gacatgttca cgaacgtgaa tcacggtgga caaggttaaa
      721 actaaccggg cgatgcgtca atggccttgt gaatcaaatg gctacttttg catcacccgg
      781 ttttatttac gcacgaatgg tgtaatcacc aatgccgatc cacttataag tggtcagtgc
      841 ttccagcccc attgggccgc gcgcgtggag tttttgtgtg cttaccgcaa cttccgcccc
      901 cagtccaaac tggccgccgt cggtaaaacg cgtagaggcg ttaacgtaaa cagcggagga
      961 atccacttcg ttaacaaaac gctgggcgtt gcgcatatcg cgggtcagga tcgcatcgga
     1021 gtgttgcgtg ccgtgttcac gaatatgggc gatggcatcg tcaagatcgc taacgatttt
     1081 gacgttcaaa tctaatgaca gaaactcatc gtcatactct tcggctttaa cagccaccac
     1141 cttcgcgggg cctgcctgca actgcgccag cgcggctgca tcagcgtgta acgtcacgcc
     1201 gctttccgcc atttgtttgc ttaatgcggg caggaagcta tcagcgatgt ttttattcac
     1261 cagcaacgtt tctaccgtat tacacgtgct cggacgctga gttttcgcgt tgacaattac
     1321 tttcagggct tcagcgatct ctgcactttc atcaacgtaa atatggcata cgcctatacc
     1381 gcctgtgatc accgggattg tcgactgctc gcggcacagt ttatgcaaac cagccccacc
     1441 gcgcgggatc agcatgtcga tgtatttatc catacgcagc atttcactga ccagcgcacg
     1501 gtcaggatta tcaattgcct gcacggcacc cgccggtaag ccgcaggatt tcagggcgtc
     1561 ctgaatcacc gctaccgttg cagcgttagt gcgacaggtt tctttgccgc cgcgcaggat
     1621 caccgcgtta ccggttttca ggcacaggga agcaacatca accgtcacgt tcgggcgcgc
     1681 ttcataaatc acgccaatca cccccagcgg tacgcgacga cgctcaagac gcaggccgct
     1741 gtccagtacg ccgccatcga ttacctgccc caccagatcg gcgagattgc acacctggcg
     1801 cacatcgtcg gcaatgcctt tcagccgtgc gggcgtcagt gccagacggt caagcatcgc
     1861 ttcgctaagg ccattggcac acgcgtctgc aacatcctgc gcgttagcgt tgaggatact
     1921 ttcgctttgt gcttccagtt catcggcgat tttttccagc acacgatttt tttcgcggct
     1981 ggagagttgc gctaatttat acgaggcttg cttcgcggca atgcccattt gttccagcat
     2041 cggccagctc cttaacgggt aatcatgtca tcacggtgaa cggcaaccgg gccgtattca
     2101 tatcccagta ttgcatcaat ttcttgcgag tggtgtccgg caatacggcg taatgcatcg
     2161 ctgttgtaac gactgacgcc gtgggcgata tcgcgacctt cgaggttgca aatacggatg
     2221 acttcaccac gcgagaaatt gccagtcacg cttttaatgc ctttcggcaa cagggagctg
     2281 ccgcgttcca gaatggcggc agttgcccct tcatctaccg tgatttcacc cgcaggcggc
     2341 gcaccgaaaa tccagcgttt acggttttca agcggagtcg cctgggcatg gaacagcgta
     2401 ccgacggaaa tgccttccat cacatcacca ataacgcccg gcttgctgcc cgcggcaata
     2461 atggtgtcga tacccgcacg gcaagccacg tcagcggcct gcaatttggt gctcataccg
     2521 ccagttccga ggcctgaaac gctatcaccg gcaatcgcgc gcagtgcgtc atcaatgccg
     2581 tacacatctt taatcagttc tgcctgcgga ttgctgcgcg ggtcagcggt atacaaacct
     2641 ttttgatcgg tcagcagcaa cagtttatcg gcacccgcca gaatcgccgc cagcgcagaa
     2701 aggttatcgt tatcaccgac cttaatctct gcagtagcga cagcatcgtt ctcattaatt
     2761 accggaacga tattgttatc gagcaacgca cgcagggtat cacgggcgtt caggaagcgt
     2821 tcacgatctt ccatatcagc acgggtcagc aacatctgcc cgacatgaat gccgtaaatt
     2881 gaaaacagct gttcccacag ttgaatcagt cgactctgcc ctaccgccgc cagcagctgt
     2941 tttgaagcga tagtcgctgg caattccggg taccccaggt gctcacgtcc ggcggcaatc
     3001 gcacccgacg tcacaataac aatccgatgc ccggcggcat gtaactgcgc gcactggcga
     3061 acaagttcaa cgatatgggc acggttcagg cggcgcgatc cgcctgttag cacactggtg
     3121 ccgagtttta ccaccagcgt ctggctgtca ctcatgattc tctgccattc aattttagga
     3181 aaaaatgata tcaaacgaac gttttagcag gactgtcgtc ggttgccaac catctgcgag
     3241 caaagcatgg cgttttgttg cgcgggatca gcaagcgtag cggcagttgt ttacgctttt
     3301 attacagatt taataaatta ccacatttta agaatattat taatctgtaa tatatcttta
     3361 acaatctcag gttaaaaact ttcctgtttt caacgggact ctcccgctga atattcgcgc
     3421 gttaattaaa atcaggaatg aaaatgaaaa agagcactct ggcattagtg gtgatgggca
     3481 ttgtggcatc tgcatccgta caggccgcag aaatatataa caaagacggt aataaactgg
     3541 atgtctatgg caaagttaaa gccatgcatt atatgagtga taatgacagt aaagatggcg
     3601 accagagtta tatccgtttt ggttttaaag gcgaaacaca aattaacgat caactgactg
     3661 gttatggtcg ttgggaagct gagtttgccg gtaataaagc ggagagtgat actgcacagc
     3721 aaaaaacgcg tctcgctttt gccgggttga aatataaaga tttgggttct ttcgattatg
     3781 gtcgtaacct gggcgcgttg tatgacgtgg aagcctggac cgatatgttc ccggaatttg
     3841 gtggcgactc ctcggcgcag accgacaact ttatgaccaa acgcgccagc agtctggcga
     3901 cgtatcggaa caccgacttc ttcggcgtta tcgatggtct gaacttaacc ctgcaatatc
     3961 aagggaaaaa cgaaaaccgc gacgttaaaa agcaaaacgg cgatggcttc ggcacgtcat
     4021 tgacatatga ctttggtggc agcgatttcg ccatcagtgg agcctatacc aactctgatc
     4081 gcaccaacga gcagaacctg caaagccgtg gcacaggcaa gcgtgcagaa gcatgggcaa
     4141 caggtctgaa atacgatgcc aataatattt atctggcaac tttctattct gaaacacgta
     4201 aaatgacgcc aatctctggc ggctttgcca ataagacgca gaactttgaa gcggtcgctc
     4261 aataccagtt tgactttggt ctgcgtccat cgctgggtta tgtcttatcg aaagggaaag
     4321 atattgaggg catcggtgat gaagatctgg tcaattatgt cgatgttggt gctacgtatt
     4381 atttcaacaa aaatatgtca gcgtttgttg attataaaat caaccaactg gatagcgata
     4441 acaaattgaa tattaataat gatgatattg tcgcggttgg catgacctat cagttttaat
     4501 ggatattgcc ggatgtgatg catccggcac atttcattca cgccgttaac ttcaccggtt
     4561 catcacgaaa atcatctgcc ggttccagct tcagattcag cgtcgccagc agctcacgca
     4621 gcttctcgtg aaattcacgc agggtgtgct ccagtcgttc aaccactttg gcatctttta
     4681 tctgaacact cgtccagtcg cctgctttat cgaacagacc aaactggtaa ctgtaggtaa
     4741 aacgggattc ctgcgcttcc agttccatcc accagcccca gaactcacgc acttccggtg
     4801 ccggtttcac gttgacgcat acagccagac aatcgaaaaa gaatcgatta tcttcgcact
     4861 taccttcacg aatatacggg cctagtgcgg taaatttttt gatcaatctg ctcttcgggt
     4921 gtccactcgg taacgtcatt gcaatctcct gttgtgatgc aactgtttta ccaaattggt
     4981 aaaatttagc aaatttttaa cacaaacgtt tttcgatcca gtctgtgatt tcctgaaggc
     5041 ctttgtcaaa attccgatac accgggttaa atgggatctc taacaattta ccgtcagcag
     5101 atgatgaggt gattaagcgt gagtcctctt ccgggctgaa cggatcgttc ttccagtagc
     5161 ctgataacat tggcgttggg cagcgacgtc ccagcaatcc ttgcactttt aatgaatagc
     5221 gattcagctc cacgcgcaac gcttcgtcgg aagcatcatg catccccaaa cggctcgcca
     5281 gaacgtcaag atacatttcc ggcacctgtt gctggcactt aaaatcactc aacagggtat
     5341 gaactaccgg accaagacag gcaaccgctt tcagacgcgg cgattcaagg tatgccagac
     5401 gcacggcaac gttagcgccg aaacggaaac caaaggccgc gacgcgagtg tgatctaccc
     5461 acggtacgtt aggcagcgcc tttaagacgt gctgatgcaa caggctggag tcctgggtga
     5521 gcttccattt tgaagaaaag cccaccgacg gcatatcaat agtcagcatc gcaatgccgc
     5581 gcggcgcaaa ataacgttca tacaggctgt aatagtccgt ctgcatcgca tccagaccac
     5641 cacacattaa tactgtcggg aacgggccat cgcctttcgg catatgcaaa aagccggtga
     5701 tgggcgcacc gccgggtacg gtaaactcca tctgccgcat cgtgcccggc agacgctgag
     5761 cggcctcttc ataggcgcgg tttgacaaag cctgcgcttg ctcggccagg ccatctcctt
     5821 tcagatgagg ataggcagca atgttgtaca acgtagccgc atgcagccag tgtcgaccgc
     5881 tgagttgcgg atcttcttcc gcacaggctt tttgttgcca caccatcgcc tgggtagccc
     5941 actcataaat ccagttgccg ccgcggtagc caatcaccgt atcgtataaa tcatcgtcgg
     6001 tacgttcggc atcgctcatc acaatacgcg cctggacatc gaggatttca cgtgggtcaa
     6061 tgccgcgcca gatccacatc agacggttaa tcatgcgata ccagtgaggg atggttttac
     6121 catcaagggc cgactgcaca ggcagttgtg cgccgtgact aaagcggcgg actagcgtcg
     6181 aggtttcagg atgtttaaag cggggtttga acagggtttc gctcaggttt gcctgtgtca
     6241 tggatgcagc ctccagaata cttactggaa actattgtaa cccgcctgaa gttaaaaaga
     6301 acaacgcccg gcagtgccag gcgttgaaaa gattagcgac cggagattgg cgggacgaat
     6361 acgacgccca tatcccacgg ctgttcgatc caggtatttt gcggaatatc aacaacataa
     6421 tcatcaacca gcggacgacc ggccggtttt gcgaagatgg tgacaaagtg cgcttttgga
     6481 tacatttcac gaatcgcaac cgcagtacca ccggtatcta ccaggtcatc aataacgatg
     6541 aagccttcgc catcgccttc tgcgcgtttc agcactttaa gctcgcgctg gttgtcgtga
     6601 tcgtagctgg aaatacaaac ggtatcgaca tgacgaatac ccagttcacg cgccagtaac
     6661 gcacccggta ccagaccgcc acggcttacg gcaataatgc ctttccattg ttcagaaggc
     6721 atcagtcggc ttgcgagttt acgtgcatgg atctgcaaca tgtcccaggt gacgatgtat
     6781 ttttcgctca tgtgaagtgt cccagcctgt ttatctacgg cttaaaaagt gttcgagggg
     6841 aaaataggtt gcgcgagatt atagagatct ggcgcactaa aaaccagtat ttcacatgag
     6901 tccgcgtctt tttacgcact gcctctccct gacgcgggat aaagtggtat tctcaaacat
     6961 atctcgcaag cctgtcttgt gttgacaaca ttttctgcta accctgtgac ctgcaatact
     7021 gttttgcggg tgatcgacaa ggagacttaa cgtgtctgaa ctgtctcaat tatctccaca
     7081 gccgctgtgg gatatttttg ccaaaatctg ttctattcct cacccgtcct atcatgaaga
     7141 gcaactcgct gaatacattg ttggttgggc aaaagagaaa ggtttccatg tcgaacgcga
     7201 tcaggtaggt aatatcctga ttcgtaaacc tgctaccgca ggtatggaaa atcgtaaacc
     7261 ggtcgtcttg caggcccacc tcgatatggt gccgcaaaaa aataacgaca ccgtgcatga
     7321 cttcactaaa gatcctatcc agccttatat tgatggcgaa tgggttaaag cacgcggcac
     7381 cacgctgggt gcggataacg gcattggtat ggcctctgca ctggcggttc tggctgacga
     7441 aaacgtggtt cacggcccgc tggaagtgct gctgaccatg accgaagaag ccggtatgga
     7501 cggtgctttt ggcttacaga gcaactggtt gcaggctgat attctgatta acaccgactc
     7561 cgaagaagaa ggtgaaatct acatgggttg tgcgggaggt atcgacttca cctccaacct
     7621 gcatttagat cgtgaagcgg ttccagctgg ttttgaaacc ttcaagttaa ccttaaaagg
     7681 tctgaaaggc ggtcactccg gcggtgaaat ccacgttggc ctgggtaatg ccaacaaact
     7741 gttggtgcgc ttcctggcgg gtcatgcaga agaactggac ctgcgtctta ttgatttcaa
     7801 cggcggcaca ctgcgtaacg ccatcccgcg tgaagccttt gcgaccattg ctgtcgcagc
     7861 tgataaagtc gacgccctga aatctctggt ggatacctat caggagatcc taaaaaacga
     7921 actggcagag aaagagaaaa atctggcatt gctgctggac tctgttgcga acgataaagc
     7981 tgccctgatt gcgaaatctc gcgatacctt tattcgtctg ctgaacgcca ccccgaacgg
     8041 tgtgatccgc aactccgacg tggcaaaagg tgtggtcgaa acctccctga acgtcggtgt
     8101 ggtgaccatg accgacaata acgtagaaat tcactgcctg atccgttctc tgatcgacag
     8161 cggtaaagac tacgtagtga gcatgctgga ttcgctgggt aagctggcag gtgcgaaaac
     8221 cgaagcaaaa ggcgcatatc ctggctggca gccggacgct aactctccgg taatgcatct
     8281 ggtacgtgaa acctatcagc gtctgttcaa caaaacgccg aacatccaga ttatccacgc
     8341 gggcctcgaa tgtggcctgt tcaaaaaacc gtatccggaa atggacatgg tgtctatcgg
     8401 gccaaccatc accggtccac actccccgga tgagcaagtt catatcgaaa gcgttggcca
     8461 ttactggaca ctgctgactg aactgctgaa agaaattccg gcgaagtaat tatttgattt
     8521 gctgccggat ggcgtttaat cgccttccgg cagtttcatc cttcattacc cttcgataaa
     8581 agccatccct gtaaacgttc gtcgcgggtt gccacgttca atctggtgat ggaacattcg
     8641 ccgctgcgat ttcagcgccg cgctattttc ctgttgctgt tgttccagct tccaggcaat
     8701 cagtaatcgc gccagccgtt tgttagcatg ctgactgcgc tctgactgaa ccttcacgct
     8761 aataccggat gccagatgcg tggcgcgtac cgccgagtcg gttttattga catgttgacc
     8821 gcccggcccc gacgaacgca gcgtctcata acggattgca tccgattgtt cctgctcatc
     8881 agcggtaaaa cgcccaatgc ccagaaacca gtttttgcgc ccatgatgag gccgatacgg
     8941 actcggacaa atccactgaa tagtgccgca ccagctttcg cttagagccc atgcgttatc
     9001 gccatccaga gaaatcagcg ccgaacgcag tgtgtcagag tagcgacccg tttctgtttc
     9061 cagcaccgtt accgcgacgt cctgtcgggt agcttcttta atcagcctgt ccagtgcttt
     9121 tctcactgcg agacaacatt cttccggccc ctgagcagag gagagttgta gcaagatcat
     9181 ccttttttcc ctccactgtt tttgagcgtc agcaccgggc gcagtcgcgc gacaggaata
     9241 attaacccag ccagcaccag acattgcacc acgctttcag ccgatttata ggcttgtggc
     9301 gcttcttcaa agatgagttg tttatcgcga caaattaccc gactgccaag ttcagtccgc
     9361 gagagttgcg tcgccgtgta tttcgctgcc agacgccctt tacactcggt gcgcccccat
     9421 ttacgccccg ccccatgcgc cagcgagtgg agcgtttttt cgttcgcaac aggtttaacc
     9481 agccaggagt aatcgccgcg tgaaccggga ataatcacca gaccgttgtc atccggcgtg
     9541 gcgcctttac gatgcaacca gccctgttga tcgccgattt ggcacgcgct aacaaagtta
     9601 tgcgccacat ccagaaccgg acttccggtg gccttaactt gttgcattat gcgcaaagcg
     9661 atcagctggc gattaatacg cgcaaacgcc agcgcatcgt catgttccgc aatataccgt
     9721 agcgcgtcgt cactgccttc aggcaaacca tgatgcgaaa acgaggcaat atgccgctgt
     9781 aaaatagact gccccaatcc cctcgagccg ctatgaacca gcagttgcag atgctgcgca
     9841 tccagaccag ccagtgcaaa cagttccgcg tcgataattt gatcaatctg ttgcagttct
     9901 gcgaagtggt taccgccacc gatggagcca agcgagttgc gccacggatg ctgtgcaaat
     9961 gctgacggca ggttttcctc cagccagctt tcttcagcaa cgtcatccag atctgataat
    10021 cgcttttcaa acttatcggc gttatatttg cgagcgagaa tatctgtttg ccatagcgcc
    10081 atgccgcagc cgatatcatt gccgaccagt gccgggtaaa aacgaccaac agagaagaac
    10141 gctgcgccaa tcgggtagcc gcgtccggga tgtaaatctg gcatccccac aacgcgctgc
    10201 atgttgggta aatttgccgt ggtgtgtaat tgttggatcg ctgaactttc gatccacagg
    10261 tcatcggatg cgatggtaaa taccgcatcg gataagggac gaatatattt gcccatgtac
    10321 cattccattt atgaataaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga
    10381 attaatcctg gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc
    10441 ttacagtttg tggaataaga aaacgcgcac atactagacg ggcgttattt tcattgcaag
    10501 ctggatttaa tgttgcggtt tatatcgcat ataaaaatta gtaaaccacg ttccccggca
    10561 ttcaacgcgc tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt
    10621 tatgctggcg tccaccgtaa gttcggattc agacttaatc aatggtttta acaaagcgct
    10681 ggcaactccg cgtcgggtgt attccggttc aacaaataac atatcgatat aatgtccaat
    10741 gcgggtaata aaaccaaccg gttgtgcatt aataactgca acccgcactt gtgatttcgc
    10801 gagtttctcc ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gctgtggaga
    10861 atagtgctga ctcgccgtca tcgtaacagc tcgaaggaaa atagcgcata gttgctgaaa
    10921 atcaccaggc tgatagtttc ttatttgtat gttattcata atataaattc aaaaacgcat
    10981 gcgaaacgct tctgccattt tcccaatttt atgcatttgg ttgttatctc gagccagttt
    11041 atgaggttca ggtttcagaa tggcaatgag caaccatgct tgctcatcaa acgcgccctg
    11101 acaatatacc aaatgcgctg cgtcattcgt tctgctgaat tggcgcaact gtggcggaaa
    11161 tggattattc acatttgcca gatgaatatg agcaactcgc tcaaatttga ttaatggcca
    11221 ggtaaacgag tcgtcgtaga gtgcatcacg accaaatata tctggcaaaa caccgtcacg
    11281 cttataggaa ataaaatccg ccgttaacgc ttcaagttcc tctgctgtaa gttgcaggcg
    11341 aataagtttt gttttgaata cccgcatcct tattccttaa agtcattgaa atcatcatcc
    11401 gtcatatcat taagatattg ttcagcgcgg cgtgtaattt cctctaatct tgcagcactc
    11461 ggacggaagc gcgcctttat gggctttttc tcctcttgtt ctgcaagcat gtccattaat
    11521 gcttcaaatg cgctggcact taagagatat cctgcggggc gattattaga aagaaccgca
    11581 acaggttgat caataaagta tttagctggg tttttacgta actcagtgat attgaccgat
    11641 ttttcagcga gaattcgatg catacagtga tctcctcata ataatgcaca ccagaatata
    11701 caaaatagta tacatcataa tcccagcacc agttgtcttt ccatttgcgg gtcaagcaac
    11761 gtcacatgca gccccaccag tcgcacaccg cgcccgccgc ggcgttcatc ccaggtttta
    11821 cgcgcggtgg cgattagatc agatttattc agccgcggcc agacgtgttc ctgggtggtt
    11881 tgctgaaaat cgtcgaactt taatttcacc ccctggcggg caatcagcaa atcaggtttt
    11941 acctttgcca aacgacgctc cagttcaggg taaagccgct cgatgatcgc ttcacattca
    12001 gaccagtgat gaatatcttc cgccatcgtg cgttccacgc cgacggattt tcgcaaccgt
    12061 tcgctgttaa cagcgcgttc gtcaatgccc tgactacgct cccacaaaat gcggccaaat
    12121 ttgccaaagc gtttaagcag catcaccaga tcacactttt gcacatcacc gcaggtccgc
    12181 agccccatcg cttccagttt tgccgccgag actttgccga cgccgggaat ttttgccagc
    12241 ggtaaggttt gcagaaatgc cggaacttct gccggcgtaa tcacaaactg gccgttgggt
    12301 ttattcatgt cggaggcgat tttggcgaga aacttgaccg gcgcaacgcc cgcagacgcc
    12361 gtcagttgca gctcgttgaa gatcgtctgg cggatttcct gggcgatgag ggtcgcagaa
    12421 ccgtggcaat ggacgctatc ggtgacatcg agataggctt catccagtga taacggttca
    12481 atgcgcgagg tatagcgcga gaaaatttcg cggatatgat ttgaggcttc tttgtaggcg
    12541 tcaaagcgcc ctggaagcaa ggtgagatgc gggcataatt tgagcgccat ccctgtcggc
    12601 atagcgctac gtacgccaaa tttacgcgcg ggataattgg cggtgctgat caccccccgt
    12661 cgttcgcggc tgccgccaat agcaataggg atatcgcgca gggcgggatt atcgcgcatc
    12721 tccaccgctg cgaaaaagca gtccatatcc acatgaatga ttttacgcat tgctcacctc
    12781 tcaatactgg taaagtatac agtgatttca gggtttgaga aatgcgtaaa gattcagcat
    12841 ttaacgcgct cgctgcgaaa gcacctgctg cttatccagc ttttgcacca gcggctgcac
    12901 cactttatcc ccatgctgac caaagtattg atacagcgta tcggacgcac ttttggtcag
    12961 caccataatc tcaatgcgcc ggttgcccgc gctttgcgga tttttggcat ccagcagcat
    13021 ctggtccgcc attgcgctta cctgcatcac tttatcttcc ggcattccag cctcttccag
    13081 cacccgacga gccgaaagcg cgcgatcgcc cgaaaggttc cagttgttgt agatattgtt
    13141 tttataggcc atcgcatcag tatgcccggt aataataatt ttgttatcga gcgagtcgaa
    13201 caccggcgca agctccacca gcagcgtttt aaagaacggc ataatcttcg cgctgccgcg
    13261 ttcaaacata ttgcggttct ggtcgtcttt aatcagcacg cgtaatccct ggggaacaat
    13321 ctccatctcc agattcgctt ccatatgcgc atcacgggcg atggtattga tactggtcgc
    13381 cagctcgcca agctcggtgg cggatttctt cttcaacagc gccgttttct cgttgacgtc
    13441 gcgcgctttt ttctccgtct cttccggcac cgccacggtg gcaggttttg gcagatgaga
    13501 cgggctgatt ttatttaacg gcgacaaccc accaccgtta aaaatcgact gcccgtgcag
    13561 cgcggcgata atgctttcac gctcggattt actgacgctg ttgacaatca actttatcgt
    13621 cgtcaccaaa ggggccgagc gtatttccga ggtttctgcc cgctttacct tagacgcgat
    13681 gcccggtaag cagatggcga ttgacgccga tcttaacgcc gggttgatca atcaggcgca
    13741 ggcgcagacc cggcgtaaag atgttgccag cgaggccgat ttctacggtg cgatggacgg
    13801 ggcatcaaaa tttgtgcgcg gggacgccat cgccgggatg atgattctgg cgatcaacct
    13861 gatcggcggc gtctgtatcg gcatcttcaa atacaacctg agcgccgacg ccgccttcca
    13921 gcaatatgtg ctgatgacta tcggcgatgg gctggtggcg caaatccctt ccctgctgct
    13981 ctccaccgcg gcggcgatta tcgtcacccg cgtcagcgat aacggtgata tcgcccatga
    14041 cgtgcgtaat cagttgctgg caagcccgtc ggttctctac accgccactg gaattatgtt
    14101 cgtgctggcg gtggtgccgg gaatgccgca tttcccgttc ctgatgttca gcgccctgct
    14161 tggttttacc ggctggcgga tgagcaagcg cccgcaagcg gcggaggcgg aagagaaaag
    14221 cctcgaaacg ctaacccgta cgatgacaga aaccagcgag caacaggtca gttgggaaac
    14281 tattccgctg atcgaaccta tcagtttaag cctcggctac aaactggtgg cgctggtgga
    14341 taaagcgcag ggcaacccgc tcacccagcg tattcgcggc gtacgtcagg tgatttccga
    14401 cggcaacggc gtgctgctgc cggagatccg tattcgggaa aacttccgcc tcaagcccag
    14461 ccagtacgcc attttcatca acggcattaa ggctgatgaa gcggatattc cggcagataa
    14521 actgatggcc ctgccctcca gcgaaaccta cggcgagata gacggcgtgc tggggaacga
    14581 cccggcgtac gggatgccgg tcacctggat tcagcccgcg cagaaagcga aggcgctgaa
    14641 tatggggtat caggtgatcg acagcgccag cgtaattgcc acccatgtga ataagattgt
    14701 gcgcagctat attcctgatt tgtttaacta tgacgatatt actcagctgc ataaccgttt
    14761 gtcgtcgatg gcaccgcgac tggcggaaga tttgagcgcg gcgctcaatt acagccagtt
    14821 gctgaaagtg taccgggcgc tactgactga aggcgtttcc ctgcgcgata tcgtcaccat
    14881 cgccaccgtg ttggtcgcca gtagtgcggt gactaaagat catattctgc tggcggccga
    14941 tgtgcgcctg gcgctgcggc gcagcataac ccatccgttc gttcgcaagc aggagctgac
    15001 ggtgtatacg ctgaataatg agctggaaaa tctgctgacc aacgtggtga atcaggcgca
    15061 gcagggcggc aaagtgatgc ttgatagcgt gccagtggat ccgaatatgc tcaaccagtt
    15121 ccagagcacg atgcctcagg tgaaggagca gatgaaagcg gcggggaaag atccggtgct
    15181 gctggtgccg ccgcagctgc gccctttgct ggcgcgctac gcaaggttgt ttgcaccagg
    15241 gctgcatgtg ctgtcgtata acgaagtgcc ggatgagctg gagttgaaga taatgggggc
    15301 gttgatgtag gcatccggca tcggtgcata ttgccggatg cggcgtgaac gccttatccg
    15361 gcctacgggt gagcgcggtt ttgtcggcat gataagacgc gtagcggcgc atctggcacc
    15421 ggtgcacaac cgccggatgc ggcgtgaacg ccttatccgg cctacgggtg agcgcggttt
    15481 tgtaggcatg ataagacgcg tctgcgtcgc atcaggcatc ggcgcacagc tataaaatga
    15541 tacgctcccc agcactaaaa tccgttacgt ctcccgccca atcgatggga tataaccctt
    15601 tcgcaacatc gcggtgaaac gttgagaatg gccaatcact cacttgtttt acccaaccat
    15661 gttttactgg atttatataa atataatcaa catgatggcg atagtctttg gtattgcgga
    15721 tagcatgttc ccaaaaacgt ggttgccaga tatttttcaa tccacaagca tgggtaaatt
    15781 gctttttaat ttcccgccag cgcgaggaaa aatcatcatc gccttcaggt aatgtccata
    15841 tgcaatgcat atgctccggc aaaactaccc aagcgttaat ttcaaaaggt ctgtctcgct
    15901 taactttaat aataacgtta cggagcgtct gaaactgggt ggtcaaaagt tggtttcgac
    15961 gattctgtaa atttaccgtg aaaaaccagg ttcccccctt gatgtaataa cgacgatatt
    16021 cagacataac gactcctttt tctcaacggt aagaagaaaa ggaaaagtcg gcaatgatgt
    16081 tgtgttaaat cgggaaagcg tctcgcaaaa aatgcacaga tttgtaggaa tgataagact
    16141 cgtagcgtcg catcagacac cggcgcacaa ccgccggatg cggcgtgaac gccttatccg
    16201 gcctacaaa
//
LOCUS       DAZGJT010000100        15834 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZGJT010000100 DAZGJT010000000
VERSION     DAZGJT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.100"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..251
                     /locus_tag="ACHKFK_003726"
     CDS             <1..251
                     /locus_tag="ACHKFK_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO9053971.1"
                     /translation="GEPGFAADDSVGVLEYVNDEGVTVREEMKPEMGDYGRVYDALYQ
                     TITNGAPNYVKESEVLTNLEILERGFEQASPSAVTLAR"
     gene            374..1069
                     /gene="yhhW"
                     /locus_tag="ACHKFK_003727"
     CDS             374..1069
                     /gene="yhhW"
                     /locus_tag="ACHKFK_003727"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO9053972.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALVKDEQSVHQIAAERRVWILVVKGNVTINGVKASTSDGLAIWDEQAISIHAN
                     SDSEILLFDLPPV"
     gene            1162..2157
                     /gene="gntR"
                     /locus_tag="ACHKFK_003728"
     CDS             1162..2157
                     /gene="gntR"
                     /locus_tag="ACHKFK_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO9053973.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDIAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            2296..2823
                     /gene="gntK"
                     /locus_tag="ACHKFK_003729"
     CDS             2296..2823
                     /gene="gntK"
                     /locus_tag="ACHKFK_003729"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO9053974.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RCNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            2827..4167
                     /gene="gntU"
                     /locus_tag="ACHKFK_003730"
     CDS             2827..4167
                     /gene="gntU"
                     /locus_tag="ACHKFK_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO9053975.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRVH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(4224..4817)
                     /gene="yhgN"
                     /locus_tag="ACHKFK_003731"
     CDS             complement(4224..4817)
                     /gene="yhgN"
                     /locus_tag="ACHKFK_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO9053976.1"
                     /translation="MNEIISATVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            5009..6112
                     /gene="asd"
                     /locus_tag="ACHKFK_003732"
     CDS             5009..6112
                     /gene="asd"
                     /locus_tag="ACHKFK_003732"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO9053977.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELANPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            6385..8571
                     /gene="glgB"
                     /locus_tag="ACHKFK_003733"
     CDS             6385..8571
                     /gene="glgB"
                     /locus_tag="ACHKFK_003733"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO9053978.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANLFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPSVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNHTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGAVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            8568..10541
                     /gene="glgX"
                     /locus_tag="ACHKFK_003734"
     CDS             8568..10541
                     /gene="glgX"
                     /locus_tag="ACHKFK_003734"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO9053979.1"
                     /translation="MTQLAIGKPTPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGVFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALME
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            10559..11854
                     /gene="glgC"
                     /locus_tag="ACHKFK_003735"
     CDS             10559..11854
                     /gene="glgC"
                     /locus_tag="ACHKFK_003735"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO9053980.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            11854..13287
                     /gene="glgA"
                     /locus_tag="ACHKFK_003736"
     CDS             11854..13287
                     /gene="glgA"
                     /locus_tag="ACHKFK_003736"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO9053981.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            13306..15753
                     /gene="glgP"
                     /locus_tag="ACHKFK_003737"
     CDS             13306..15753
                     /gene="glgP"
                     /locus_tag="ACHKFK_003737"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO9053982.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNDLQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYERQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3657 a         3944 c         4377 g         3856 t
ORIGIN      
        1 cgggcgaacc aggattcgca gcggatgatt cggtcggtgt gctggagtat gtcaatgacg
       61 aaggcgtgac ggtaagagaa gagatgaagc cagagatggg cgattatggg cgcgtttatg
      121 atgcgttgta tcaaaccatc accaacggtg cgccaaatta cgtcaaggaa tctgaagttc
      181 ttacgaatct ggaaatcctt gaacgcggtt ttgagcaagc ttctccctcc gcagtgactc
      241 tcgcgaggta agtttgatgg cccctcgaat agttcaattt ttttgaacag aggggtcaat
      301 tttcaccctc tatcatccca ggcggatcgg gtccacacta agcccatcga aatcattcag
      361 ggggcgaata caaatgatct acttacgcaa agcaaatgaa cgcggtcatg caaatcatgg
      421 ctggctggac tcctggcata ctttctcttt tgccaactat tacgatccga actttatggg
      481 cttctctgcg ctgcgcgtga ttaacgacga cgtgattgaa gcagggcagg gcttcggcac
      541 tcacccgcat aaagatatgg aaattttgac ctacgtgctg gaaggtactg ttgagcacca
      601 ggacagcatg ggcaataaag agcaggttcc ggcgggcgag ttccagatta tgagtgcggg
      661 tacgggtatt cgtcactcag agtacaaccc aagcagcacc gagcgtctgc atctgtatca
      721 gatctggatc atgcctgaag aaaacggtat tacgccgcgt tatgaacagc gtcgcttcga
      781 tgccgtgcag ggcaaacaac tggtgctttc gccggatgcg cgagatggtt cattgaaagt
      841 gcatcaggat atggaactgt atcgttgggc gctggtgaaa gatgagcagt cggtgcatca
      901 gattgccgct gaacgccgcg tctggatcct ggtggtgaaa ggtaatgtca ccattaacgg
      961 cgtaaaagct tcgaccagcg atggtctggc aatctgggat gagcaggcga tttccatcca
     1021 tgctaatagc gacagcgaaa tcctgctgtt cgatttaccg ccagtctaaa tttatctcac
     1081 aaaccttcct gaggaatcag gaaggttgtg atgcgtccgt gttaaactaa gagaatctat
     1141 ctcttttgta ccttcaggac gatgaaaaag aaaagacccg tacttcagga tgtggctgac
     1201 cgtgtaggcg tgaccaaaat gacggtcagc cgttttttac gcaacccgga gcaggtttcc
     1261 gtcgctctac gcggcaagat tgccgccgct cttgatgaac tgggttatat tcccaatcgc
     1321 gcgcccgata tactctctaa cgccaccagc cgggcgattg gcgtcctgtt accttctctc
     1381 accaaccagg ttttcgcgga agtattacgc ggaatcgaaa gcgtcaccga cgcgcacggt
     1441 tatcaaacca tgctggcgca ctacggttat aaaccggaaa tggagcaaga acgcctcgaa
     1501 tcaatgctct catggaatat cgacggcctg atcctcaccg aacgtaccca cacgccgcgt
     1561 accttaaaga tgattgaagt ggcggggatt ccggtggtgg agttgatgga cagtaagtcg
     1621 ccgtgcctcg atatcgccgt cggttttgat aactttgaag cggcacgcca gatgaccacc
     1681 gccattattg ctcgcgggca tcgtcacatt gcctatctcg gcgcacgcct cgacgaacgt
     1741 actatcatca aacagaaggg atacgaacag gcgatgctgg atgcaggcct ggtgccgtat
     1801 agcgtgatgg ttgagcaatc ttcttcttac tcttccggta ttgaactgat tcgccaggcg
     1861 cggcgggaat atccgcagct ggatggcgtg ttctgtacta atgatgacct ggcggtcggc
     1921 gcggcgtttg aatgtcagcg tctggggttg aaagttcctg acgatatagc gattgccggt
     1981 ttccatggtc atgacattgg tcaggtgatg gagccacggc tggcgagtgt gctgacgccg
     2041 cgtgagcgga tgggcagcat tggcgctgag cgcctgctgg cgcgtattcg tggcgaatct
     2101 gtgacaccga aaatgttaga tttaggtttc accttgtcac cgggcggatc tatttaagcc
     2161 tacaaatttg aagtagctca cacttataca cttaaggcac ggatggatat tgcttctgat
     2221 attgtccggc tggacaatgt taccgataac agttacccgt aacattttta attcttgtat
     2281 tgtgggggca ccactttgag cacgactaac catgatcacc acatttacgt cttgatgggc
     2341 gtatcgggca gcggcaaatc tgcggtcgcc agtgaagtgg cgcatcaact tcatgccgcg
     2401 tttcttgatg gcgatttcct ccatccgcgc tgcaatatcg aaaaaatggc gtctggcgaa
     2461 ccgctgaatg acgacgatcg caaaccgtgg ttgcaggcgc tgaacgacgc cgcgtttgct
     2521 atgcagcgca ccaataaagt atcgctgatc gtctgttctg cactgaaaaa acactatcgc
     2581 gacttgctgc gtgaaggtaa tcctaatctc tctttcatct acctgaaagg cgattttgat
     2641 gtgattgaaa gccgcctgaa agcgcgcaaa ggccatttct ttaaaaccca aatgctggtg
     2701 acgcagtttg aaacgctgca ggagccgggt gcggacgaaa ccgatgtact ggtggtggat
     2761 attgatcaac cgctggaagg tgttgtggca agcaccattg aggttattaa aaaaggcaaa
     2821 taagtagtga ctacattaac gcttgtttta acagcagtag ggtctgtttt actgctgctg
     2881 tttttagtca tgaaggcgcg tatgcacgct ttcctggctt taatggtggt gtccatgggg
     2941 gctggccttt tttccggtat gccgctcgat aaaatcgcag cgacgatgga aaaagggatg
     3001 ggaggcaccc tcggcttcct ggcggtggtt gtcgccctgg gagccatgtt tggtaagatc
     3061 ttacatgaaa ccggcgcggt cgatcagatt gccgtcaaaa tgctcaaatc cttcggtcac
     3121 agccgcgtgc attatgccat cggccttgcg gggctggttt gtgcgctacc gctgttcttt
     3181 gaagtggcga ttgttctgct gattagcgtt gctttctcaa tggcgcgcca caccggcacg
     3241 aacctggtga agctggtaat cccattattt gcaggcgtgg cggcagcggc tgcgttcctg
     3301 gtgcctgggc cagcgccaat gctgctggca tcgcagatga acgccgactt tggctggatg
     3361 atcctgattg gcctgtgcgc ggcaattccg ggaatgatta ttgccgggcc gctgtggggt
     3421 aatttcatca gccgctacgt ggagttgcat attcctgacg acatcagcga accgcatctc
     3481 ggcgaaggca aaatgccgtc cttcggattc agcctgtcgc tgatcctgtt gccactggtg
     3541 ctggtggggc tgaaaaccat tgccgcgcgt tttgtgccag aaggctctac tgcttacgaa
     3601 tggttcgagt ttattggtca tccgtttacc gcgattctgg ttgcttgcct ggtggcgatt
     3661 tacggtctgg caatgcgtca gggtatgcca aaagacaaag tgatggagat ttgcggtcac
     3721 gcgctgcaac cggcggggat cattctgctg gtgattggtg cgggcggcgt gttcaaacag
     3781 gtgctggttg actctggtgt cggtccggca ctgggcgaag cgttaaccgg catgggcctg
     3841 ccgattgcca tcacctgctt tgtgctggct gcggcggtgc gtatcattca gggttctgcc
     3901 accgttgcct gtttaacggc ggtgggactg gtgatgccgg ttattgaaca actgaactac
     3961 tccggtgcgc aaatggcggc gctgtcgatt tgtatcgccg gtggttcgat tgttgtcagc
     4021 cacgttaacg acgctggttt ctggttgttc ggtaaattta ccggcgcgac cgaagccgaa
     4081 acgctgaaaa cctggaccat gatggaaacc atactcggca ctgttggtgc catcgttggg
     4141 atgattgctt tccagctgtt gagttaagtt tgttcgcccg gtagttgtga cgctaccggg
     4201 ctcttttcga aaaactctcc tcgttacccc ttcatccaca ttcgaatgcc gtcgaggaac
     4261 atctgagttg ccatcatcac cagaatcaat cccatcaggc gttcaagtgc gttcacccct
     4321 ttctcgccca gcagacgtaa aaatagcgaa gactgtagca ggatgacaaa ggtgccgccc
     4381 caggccagca gcagagcaat caccagatgt cccatctgat tcgggtactg atgagacaac
     4441 aacatcagcg tggcgagaat agtcggcccg gcgaccagcg gaattgccaa cggcacgata
     4501 aatggctctt cacctgccgg aagcccgctg ctatttcctg aagcgctggg gaaaatcatc
     4561 ttaatggcga tcagaaacag aatgatgccg ccagaaatgg agacggtttc tgcccgcagg
     4621 ctaagaaatg ccagaatttt ctcgcccgca aacaggaaca ccagcatcac aaggagagca
     4681 ataagcaact ctcgcaccat gattgccctc cgtcttttcg gttcagtatg tttcagtacg
     4741 gacatgaaaa taggtaggtt tccgagcgga tccataatca ggatcaataa aactgttgca
     4801 gaaatgattt cattcataac tcaaattccc tgataattgc cgcggacttt ctgcgtgcta
     4861 acaaagcagg ataagtcgca ttactgatgg cttcgctatc attgattaat ttcacttgcg
     4921 actttggctg ctttttgtat ggtgaaagat gtgccaggag gagaccggca catttataca
     4981 gcacacatct ttgcaggaaa aaacgcttat gaaaaatgtt ggttttatcg gctggcgcgg
     5041 tatggtcggc tccgttctca tgcaacgcat ggttgaagag cgcgacttcg acgccattcg
     5101 ccctgtcttc ttttctactt ctcagcttgg tcaggctgcg ccgtcttttg gcggaaccac
     5161 tggcacactt caggatgcct ttgatctgga ggcgctaaag gccctcgata tcattgtgac
     5221 ctgtcagggc ggcgattata ccaacgaaat ctatccaaag cttcgtgaaa gcggatggca
     5281 aggttactgg attgacgcag catcatctct gcgcatgaaa gatgacgcca tcatcattct
     5341 tgaccccgtc aatcaggacg tcattaccga cggattaaat aatggcatca ggacttttgt
     5401 tggcggtaac tgtaccgtaa gcctgatgtt gatgtcgctg ggtggtttat tcgccaatga
     5461 tcttgttgat tgggtgtccg ttgcaaccta ccaggccgct tccggcggtg gtgcgcgaca
     5521 tatgcgtgag ttattaaccc aaatgggcca tctgtatggc catgtggcag atgaactcgc
     5581 gaacccgtct tctgctattc tcgatatcga acgcaaagtc acaaccttaa cccgtagcgg
     5641 tgagctgccg gtagataact ttggcgtgcc gctggcgggt agcctgattc cgtggatcga
     5701 caaacagctt gataacggtc agagccgcga agagtggaaa gggcaggcgg aaaccaacaa
     5761 gatcctcaac acatcttccg taattccggt agatggttta tgtgtgcgtg tcggggcatt
     5821 gcgctgccac agccaggcat tcactattaa attgaaaaaa gatgtgtcta ttccgaccgt
     5881 ggaagaactg ctggctgcgc acaatccgtg ggcgaaagtt gttccgaacg atcgggaaat
     5941 cactatgcgt gagctaaccc cagctgccgt taccggcacg ctgaccacgc cggtaggccg
     6001 cctgcgtaag ctgaatatgg gaccggagtt cctgtcagcc tttaccgtgg gcgaccagct
     6061 gctgtggggg gccgcggagc cgctgcgtcg gatgcttcgt caactggcgt aatctttatt
     6121 cattaaatct ggggcgcgat gccgcccctg ctagtgcgta atacaggagt aagcgcagat
     6181 gtttcatgat ttaccgggag ttaaatagag cattggctat tctttaaggg tggcggaata
     6241 cacgagtatt cacagcctta cctgaagtga ggacgacgca gagaggatgc acagagtgct
     6301 gcgccgttca ggtcaaaaaa atgtcacaac cagaagtcaa aaattcagtt ggatggggtg
     6361 acacaataaa acaggaagac aagcatgtcc gatcgtatcg atagagacgt gattaacgcg
     6421 ctaattgcag gccattttgc ggatcctttt tccgtactgg ggatgcataa aaccaccgcg
     6481 ggactggaag tccgtgccct tttacccgac gctaccgatg tgtgggtgat tgaaccgaaa
     6541 accgggcgca aactcgcaaa actggagtgt ctcgactcac ggggattctt tagcggtgtc
     6601 attccgcgac gtaagaattt tttccgctat cagttggctg ttgtctggca tggtcagcaa
     6661 aacctgattg atgatcctta ccgttttggc ccgctaatcc aggaaatgga tgcctggcta
     6721 ttatctgaag gtactcacct acgtccgtat gaaaccttag gcgcgcatgc agatactatg
     6781 gatggcgtca caggtacgcg tttttctgtc tgggctccaa acgcccgtcg ggtctcggtg
     6841 gttgggcaat tcaactactg ggacggtcgc cgtcatccga tgcgactgcg taaagagagc
     6901 ggaatctggg aactgtttat ccccggggcg cataacggtc agctctataa atacgagatg
     6961 attgatgcca atggcaactt gcgcctgaag tccgaccctt atgccttcga agcgcaaatg
     7021 cgcccggaaa ccgcgtctct tatttgcggg ctgccggaaa aggttgtgca gactgaagag
     7081 cgcaaaaaag cgaatctgtt tgatgcgcca atctctattt atgaagttca ccttggctcc
     7141 tggcgtcgcc acaccgacaa caatttctgg ttgagctacc gtgagctggc cgatcaactg
     7201 gtgccttatg ctaaatggat gggctttacc catctcgagc tactgcccat taacgagcat
     7261 cccttcgatg gcagttgggg ttatcagcca accggcctgt atgcaccgac ccgccgtttt
     7321 ggtactcgcg acgacttccg ttatttcatt gatgccgcac acgcagctgg tctgaacgtg
     7381 attctcgact gggtgccagg ccacttcccg accgatgact ttgcgcttgc cgaatttgat
     7441 ggcacgaact tgtatgaaca cagcgatccg cgcgaaggct atcatcagga ctggaacacg
     7501 ctgatctaca actatggtcg ccgtgaagtc agtaacttcc ttgtcggtaa cgcactttac
     7561 tggattgaac gttttggtat tgatgcgctg cgcgtcgatg cggtggcgtc aatgatttat
     7621 cgcgactaca gccgtaaaga gggggagtgg atcccgaacg aatttggcgg tcgcgagaat
     7681 cttgaagcga ttgaattctt gcgtaatacc aaccgtattc ttggtgagca ggtttccggt
     7741 gcagtgacaa tggcggagga gtctaccgat ttccctagcg tttctcgtcc gcaggatatg
     7801 ggcggtctgg gcttctggta caagtggaac ctcggctgga tgcatgacac cctggactac
     7861 atgaagctcg acccgattta tcgtcagtat catcacgata aactgacctt cgggatgctc
     7921 tacaactaca ctgaaaactt cgtcctgccg ttgtcgcatg atgaagtggt ccacggtaaa
     7981 aaatcgattc tcgaccggat gccgggcgac gcatggcaga aattcgctaa cctgcgcgcc
     8041 tactacggct ggatgtgggc attcccgggc aagaaactac tgttcatggg gaacgaattt
     8101 gcccagggcc gcgagtggaa ccatgacgcc agcctcgact ggcatctgtt ggaaggcggc
     8161 gataactggc accacggtgt ccagcgtctg gtgcgcgatc tgaaccacac ctaccgccac
     8221 cataaagcaa tgcatgaact ggattttgac ccgtacggct ttgaatggct ggtggtggat
     8281 gacaaagaac gctcggtgct gatctttgtg cgtcgcgata aagagggtaa cgaaatcatc
     8341 gttgccagta actttacacc ggtgccgcgt catgattatc gcttcggtat taaccagccg
     8401 ggtaaatggc gtgaaatcct caataccgat tccatgcact atcacggcag taatgcaggc
     8461 aatggcggcg cagtacacag cgatgagatt gccagccacg gtcgtcagca ttcactgagc
     8521 ctgacgctac cacctctggc cactatctgg ctggttcggg aggcagaatg acacaactcg
     8581 ccattggcaa acccactccc ctcggcgcgc attacgacgg tcagggcgtc aacttcacac
     8641 ttttctccgc tcatgccgag cgggtagagc tgtgtgtctt tgatgctaat ggccaggaac
     8701 atcgctatga cttgccaggg cacagtggcg acatttggca cggttatctg ccggatgcgc
     8761 gcccgggttt gcgttatggt tatcgcgttc atggcccctg gcaacctgcc gaggggcatc
     8821 gctttaaccc ggcgaagttg ttgattgatc cttgcgcgcg gcaaattgac ggggagttta
     8881 aagataaccc gctgctgcac gccggtcata atgaacctga ctatcgcgac aacgccgcca
     8941 ttgcgccgaa atgcgtagtg gtggttgatc actatgactg ggaagatgat gccccgccgc
     9001 gcacgccgtg gggcagcacc atcatttatg aagcccatgt caaaggatta acgtatctgc
     9061 acccggagat cccggtcgag atccgcggca cttataaagc cctggggcat ccggtgatga
     9121 tcaactattt gaaacagttg ggcattactg cgctggagtt gctgccggtg gcacaatttg
     9181 ccagtgaacc acgtctgcaa cgcatggggc ttagtaacta ctgggggtac aacccggtgg
     9241 cgatgtttgc gctgcatccg gcgtatgcct gctcgccaga aacggcgctg gatgagtttc
     9301 gcgatgcaat caaagcactg cataaagcgg gtatcgaagt cattcttgat atcgtgctca
     9361 accatagtgc ggaactggac ctcgacggcc cgttattctc gctgcgtggg atcgacaacc
     9421 gtagctatta ttggataaga gaagacggcg attatcacaa ctggaccggt tgtggtaaca
     9481 cgctcaattt gagtcatccg gcggtggtag attatgccag cgcctgcctg cgttattggg
     9541 tagaaacctg ccacgtcgat ggtttccgct ttgatctggc ggcagtcatg ggccgtacgc
     9601 cagagtttcg tcaggatgcg ccgttgttta ccgctatcca aaactgcccg gtgctctcgc
     9661 aggtgaagtt aattgctgaa ccgtgggata tcgctcctgg tggttatcag gtgggaaatt
     9721 tcccgccgct gtttgccgag tggaacgatc atttccgcga tgcagcccgt cgtttctggc
     9781 tgcattatga tttacctctg ggagtgtttg ccgggcgttt tgctgcctcc agcgatgttt
     9841 ttaaacgtaa tggtcgtctg ccgagtgccg cgattaatct cgtcaccgcg catgacggtt
     9901 ttacgcttcg cgactgcgtt tgcttcaacc ataaacacaa tgaagcaaac ggagaagaaa
     9961 atcgcgacgg gaccaacaac aattacagta acaatcatgg taaagaaggg ttaggcggta
    10021 ctcttgatct ggttgaacgg cggcgcgaca gcattcacgc cctgttaaca acgttgttgc
    10081 tctcccaggg tacgccgatg ttactggccg gtgacgaaca tggtcacagc cagcatggca
    10141 ataacaatgc ctactgtcag gataaccaat taacctggtt ggactggtcg caggcaagca
    10201 gtggtttaac cgcatttacc gccgcgttaa tccatctgcg caagcgcatt cccgctttga
    10261 tggagaatcg ctggtgggaa gaaggcgacg gcaatgtccg ttggctaaat cgatatgctc
    10321 aacctttaag cacggatgag tggcaaaacg ggccgaaaca gctgcaaatt ctgctctcgg
    10381 atcgcttttt gatcgcaatt aacgccacgc ttgaggtaac agagattgtt ttacctgctg
    10441 gggagtggca cgccattccc ccattcgctg gagaggataa cccagtgatt acggctgtct
    10501 ggcagggacc tgcacacgga ttgtgtgtgt tccagagatg ataaaaaagg agttagtcat
    10561 ggttagttta gagaagaacg atcacttaat gttggcgcgc cagctgccat tgaaatctgt
    10621 tgccctgata ctggcgggag gacgtggtac ccgcctgaag gatttaacca ataagcgagc
    10681 aaaaccggcc gtacacttcg gcggtaagtt ccgcattatc gactttgcgc tgtctaactg
    10741 catcaactcc gggatccgtc gtatgggcgt gatcacccag taccagtccc acactttggt
    10801 gcagcacatc cagcgcggct ggtcattctt caatgaagaa atgaacgagt ttgtcgatct
    10861 gctgccagca cagcagagaa tgaaagggga aaactggtat cgcggcaccg cagatgcggt
    10921 cacccaaaac ctcgacatta tccgccgtta taaagcggaa tacgtggtga tcctggcggg
    10981 cgaccatatc tacaagcaag actactcgcg tatgcttatc gaccacgtcg aaaaaggcgc
    11041 acgttgcacc gttgcttgta tgccagtacc gattgaagaa gcctccgcgt ttggcgttat
    11101 ggcggttgat gagaacgata aaattatcga attcgttgaa aaaccagcta acccgccgtc
    11161 aatgccgaac gatccgagca aatctctggc gagtatgggt atttacgtct ttgacgccga
    11221 ctatctgtat gaactgctgg aagaagacga tcgcgatgag aactccagcc acgactttgg
    11281 caaagatttg attcccaaga tcaccgaagc cggtctggcc tatgcgcacc cgttcccgct
    11341 ctcttgcgta caatccgacc cggatgccga gccgtactgg cgcgatgtgg gtacgctgga
    11401 agcttactgg aaagcgaacc tcgatctggc ctctgtggtg ccggaactgg atatgtacga
    11461 tcgcaattgg ccaattcgca cctacaatga atcattaccg ccagcgaaat tcgtgcagga
    11521 tcgctccggt agccacggga tgacccttaa ctcactggta tccggcggtt gtgtgatctc
    11581 cggttcggtg gtggtgcagt ccgttctgtt ctcgcgcgtt cgcgtgaatt cattctgcaa
    11641 cattgattcc gccgtattgt taccggaagt atgggtaggt cgctcgtgcc gtctgcgccg
    11701 ctgcgtcatc gatcgtgctt gtgttattcc ggaaggcatg gtgattggtg aaaacgcaga
    11761 ggaagatgca cgtcgtttct atcgttcaga agaaggcatc gtgctggtaa cgcgcgaaat
    11821 gctacggaag ttagggcata aacaggagcg ataatgcagg ttttacatgt atgttcagag
    11881 atgttcccgc tgcttaaaac cggcggtctg gctgatgtta ttggggcatt acccgcagca
    11941 caaatcgcag acggcgttga cgctcgcgta ctgttgcctg catttcccga tattcgccgt
    12001 ggcgtgaccg atgcgcaggt agtatcccgt cgtgatacct tcgccggaca tatcacgctg
    12061 ttgttcggtc attacaacgg ggttggcata tacctgattg acgcgccgca tctctatgat
    12121 cgtccgggaa gcccgtatca cgataccaac ttatttgcct ataccgacaa cgtattgcgt
    12181 tttgcgctgc tggggtgggt tggggcagaa atggccagcg ggcttgaccc attctggcgt
    12241 cctgatgtgg tgcatgcgca cgactggcat gcaggccttg cgcctgcgta tctggcggcg
    12301 cgcgggcgtc cggcgaagtc ggtgtttact gtgcacaacc tggcctatca aggcatgttt
    12361 tatgcacatc acatgaatga catccaattg ccatggtcat tctttaatat tcatgggctg
    12421 gaattcaacg gacaaatctc tttcctgaag gccggtctgt actatgccga tcacattacg
    12481 gcagtcagcc caacctacgc tcgcgagatc accgaaccgc agtttgccta cggtatggaa
    12541 ggtctgttgc aacagcgtca ccgtgaaggg cgtctttccg gcgtactgaa cggcgtggac
    12601 gagaaaatct ggagtccaga gacggactta ctgttggcct cgcgttacac ccgcgatacg
    12661 ttggaagata aagcggaaaa taagcgccag ttacaaatcg caatggggct taaggttgac
    12721 gataaagtgc cgctttttgc agtggtgagc cgtctgacca gccagaaagg tctcgacctg
    12781 gtgctggaag ccttacctgg tcttctggag cagggcgggc agctggcgct actcggcgcg
    12841 ggcgatccgg tgctgcagga aggtttcctt gcggcggcag cggaataccc cggccaggtg
    12901 ggcgttcaga ttggctatca cgaagcattt tcgcatcgca ttatgggcgg cgcggacgtc
    12961 attctggtgc ccagccgttt tgaaccgtgc ggcttaacgc aactttatgg attgaagtac
    13021 ggtacgctgc cgttagtgcg gcgcaccggt gggcttgctg atacggtttc tgactgttct
    13081 cttgagaacc ttgcagatgg cgtcgccagt gggtttgtct ttgaagatag taatgcctgg
    13141 tcgctgttac gggctattcg acgtgctttt gtactgtggt cccgtccttc actgtggcgg
    13201 tttgtgcaac gtcaggctat ggcaatggat tttagctggc aggtcgcggc gaagtcgtac
    13261 cgtgagcttt actatcgctt gaaatagttt tcaggaaacg cctatatgaa tgctccgttt
    13321 acatattcat cgcccacgct tagcgtagaa gctcttaagc actctatcgc ttacaagctg
    13381 atgtttacga ttggcaagga tccggtcgtc gccaataaac atgaatggct gaacgcaacg
    13441 ttatttgctg tgcgcgatcg tctcgtggag cgctggttac gctcaaaccg tgcccagttg
    13501 tcgcaagaaa ctcgtcaggt ttactacctg tcgatggagt ttttgattgg ccgtacgctt
    13561 tccaacgcca tgttgtcgct aggaatttac gaagatgtac agggcgcact ggaagcgatg
    13621 ggattaaatc tcgaagagct gattgatgaa gaaaatgacc cgggcctcgg taacggtggc
    13681 ctgggccgtc tggcggcttg cttcctcgat tctctggcga cgttagggtt gccggggcgc
    13741 ggttacggca tccgctatga ctacggtatg ttcaagcaga acatcgttaa cggtagccag
    13801 aaagagtcgc ctgactactg gctggaatac ggtaacccgt gggaattcaa acgccacaac
    13861 acgcgctata aagtccgttt tggcggtcgc attcagcagg aaggtaaaaa aacgcgctgg
    13921 attgaaaccg aagagattct gggagtagct tacgatcaga taatcccagg ttacgacacc
    13981 gacgcgacca acacgttgcg tttgtggagt gcccaagcca gtagcgaaat taacctcggt
    14041 aaattcaacc agggcgacta cttcgcggca gtggaagata aaaaccactc cgagaacgta
    14101 tctcgcgtac tgtatccgga cgactccacc tactccgggc gtgagctacg actgcgtcag
    14161 gaatacttcc tggtttcctc gaccattcag gacattttaa gccgccatta tcagttgcat
    14221 aaaacctacg ataacctggc ggataaaatt gcgattcacc tcaacgatac tcatccggtg
    14281 ctgtcgattc ctgagatgat gcgtctgctg atcgatgagc accaatttag ctgggacgac
    14341 gcgtttgaag tgtgttgtca ggtcttctcc tacaccaacc acacgctaat gagcgaggcg
    14401 ctggaaacct ggccagtcga tatgctgggt aaaattctgc cgcgtcacct gcagatcatc
    14461 tttgaaatca acgactattt cctgaaaacc ttgcaggaac agtatccgaa cgataccgat
    14521 ctgctgggac gggcgtcgat cattgatgaa tccaacggtc gtcgtgtgcg tatggcctgg
    14581 ctggcggttg tggtgagcca caaagttaac ggtgtatcgg agctgcactc taacctgatg
    14641 gtgcaatcgc tatttgccga ctttgcgaaa atcttcccgg gtcgtttcac caacgtcacc
    14701 aacggtgtga cgccgcgtcg ctggctggcg gtagcgaacc catcgctttc agccgtgttg
    14761 gacgaacacc tgggccgcaa ctggcgcacc gaccttagcc tgcttaatga tctgcaacaa
    14821 cactgtgatt tcccaatggt taatcacgcg gtgcatcagg cgaagctgga gaacaaaaag
    14881 cgtctggcgg agtatatcgc ccagcagctg aatgtggtgg taaatccgaa agcgctgttc
    14941 gatgtgcaaa tcaaacgtat tcacgaatac aaacgtcaat tgatgaatgt gttgcacgtg
    15001 atcacccgct ataaccgcat caaggccgac ccggatgcga agtgggtacc gcgcgtgaat
    15061 atttttggcg gtaaggcggc ttcggcctat tacatggcga agcacattat tcatttgatc
    15121 aatgacgtag cgaaagtgat caacaacgat ccgcagattg gcgacaagct gaaagtcgtg
    15181 ttcatcccga actacagtgt tagcctggcg cagttgatca ttccggcggc cgatctgtct
    15241 gaacagattt cgctggcggg gacggaagct tccggcacca gtaacatgaa gtttgcgctt
    15301 aacggcgcgc tgactatcgg tacgctggac ggtgcgaatg tcgagatgct ggatcatgtc
    15361 ggtgctgaca atatctttat ctttggtaac acagcggaag aagtggaaga actgcgtcgt
    15421 cagggctaca aaccgcgtga atactacgag aaagatgagg agctgcatca ggtgctgacg
    15481 caaatcggca gcggtgtatt cagtccggaa gatccgggtc gctatcgcga tctggtcgat
    15541 tcgctgatca acttcggcga tcactaccag gtactggcgg attatcgcag ctatgtcgat
    15601 tgtcaggata aagtcgacga actctacgag cgtcaggaag agtggaccgc aaaagcgatg
    15661 ctgaacattg ccaatatggg ctacttctct tctgaccgta cgatcaaaga gtacgccgat
    15721 catatctggc atattgatcc ggtgagattg taagttcaaa tacggctaaa taccgtgtgt
    15781 tccgctgaag taatacagcg tggaactcac ggtatttcta tacacaataa acaa
//
LOCUS       DAZGJT010000101        15536 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZGJT010000101 DAZGJT010000000
VERSION     DAZGJT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.101"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            382..837
                     /gene="csgB"
                     /locus_tag="ACHKFK_003738"
     CDS             382..837
                     /gene="csgB"
                     /locus_tag="ACHKFK_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO9053983.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            878..1336
                     /gene="csgA"
                     /locus_tag="ACHKFK_003739"
     CDS             878..1336
                     /gene="csgA"
                     /locus_tag="ACHKFK_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO9053984.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGGNHGGGGNNSGPNS
                     ELNIYQYGGGNSALALQADARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSA
                     TLDQWNGKDSTMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            1394..1726
                     /gene="csgC"
                     /locus_tag="ACHKFK_003740"
     CDS             1394..1726
                     /gene="csgC"
                     /locus_tag="ACHKFK_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO9053985.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQLIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPSSSEKS"
     gene            1847..2158
                     /gene="ymdA"
                     /locus_tag="ACHKFK_003741"
     CDS             1847..2158
                     /gene="ymdA"
                     /locus_tag="ACHKFK_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO9053986.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFNGQIVEPACDV
                     STQSSPVEMNCPQNGSVPRKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            2253..2786
                     /gene="ymdB"
                     /locus_tag="ACHKFK_003742"
     CDS             2253..2786
                     /gene="ymdB"
                     /locus_tag="ACHKFK_003742"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO9053987.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            2728..4209
                     /gene="clsC"
                     /locus_tag="ACHKFK_003743"
     CDS             2728..4209
                     /gene="clsC"
                     /locus_tag="ACHKFK_003743"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO9053988.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPQLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDNILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHDDAMTHRYLRKMESSPFINHLVDGTLSLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHSKKEIILKKEPATSFWKRVMVKLASILPVEWLL"
     gene            complement(4217..5374)
                     /gene="mdoC"
                     /locus_tag="ACHKFK_003744"
     CDS             complement(4217..5374)
                     /gene="mdoC"
                     /locus_tag="ACHKFK_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO9053989.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            5768..7303
                     /gene="mdoG"
                     /locus_tag="ACHKFK_003745"
     CDS             5768..7303
                     /gene="mdoG"
                     /locus_tag="ACHKFK_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO9053990.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            7296..9839
                     /gene="mdoH"
                     /locus_tag="ACHKFK_003746"
     CDS             7296..9839
                     /gene="mdoH"
                     /locus_tag="ACHKFK_003746"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO9053991.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDVWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            10011..10238
                     /gene="yceK"
                     /locus_tag="ACHKFK_003747"
     CDS             10011..10238
                     /gene="yceK"
                     /locus_tag="ACHKFK_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO9053992.1"
                     /translation="MRLIVMSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDVHHGPYE"
     gene            complement(10239..10613)
                     /gene="msyB"
                     /locus_tag="ACHKFK_003748"
     CDS             complement(10239..10613)
                     /gene="msyB"
                     /locus_tag="ACHKFK_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MysB family protein"
                     /protein_id="HFO9053993.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAENANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQDENTLHEWDEGE
                     FQLEPPLDTEEGRTAADEWDER"
     gene            complement(10696..11922)
                     /gene="mdtG"
                     /locus_tag="ACHKFK_003749"
     CDS             complement(10696..11922)
                     /gene="mdtG"
                     /locus_tag="ACHKFK_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO9053994.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQISN"
     gene            complement(12094..13014)
                     /gene="lpxL"
                     /locus_tag="ACHKFK_003750"
     CDS             complement(12094..13014)
                     /gene="lpxL"
                     /locus_tag="ACHKFK_003750"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO9053995.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIILPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            13239..14291
                     /gene="trhO"
                     /locus_tag="ACHKFK_003751"
     CDS             13239..14291
                     /gene="trhO"
                     /locus_tag="ACHKFK_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO9053996.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKVTRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPLFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPEPTE"
     gene            complement(14333..14908)
                     /gene="yceI"
                     /locus_tag="ACHKFK_003752"
     CDS             complement(14333..14908)
                     /gene="yceI"
                     /locus_tag="ACHKFK_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO9053997.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IELKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(14912..15478)
                     /gene="yceJ"
                     /locus_tag="ACHKFK_003753"
     CDS             complement(14912..15478)
                     /gene="yceJ"
                     /locus_tag="ACHKFK_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO9053998.1"
                     /translation="MSFTNTPERYGVISAALHWISAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
BASE COUNT         4015 a         3668 c         3981 g         3872 t
ORIGIN      
        1 gaaaaacgtt cttgtttttt ctccatacca ccgtggacaa ttttttactg caaaaagacg
       61 aggtttgtca cggcttgtgc gcaagacata tcgcagcaat cagcgacggg caagaagaat
      121 gactgtctgg tgatttttga tagcggaaaa cggagattta aaagaaaaca aaatattttt
      181 gcgtagataa cagcgtattt acgtgggttt taatactttg gtatgaacaa aaaaaaatac
      241 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      301 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      361 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      421 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttt
      481 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      541 actaataata gtgctcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      601 gaaggtagta gcaaccgggc aaagattgat cagacaggag attataacct tgcatatatt
      661 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      721 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      781 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      841 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
      901 aattgcagca atcgtattct ctggtagcgc tctggcaggt gttgttcctc agtacggcgg
      961 cggcggtggc aaccacggtg gtggcggtaa taacagcggc ccgaattcag agctgaatat
     1021 ttaccagtac ggtggcggta actctgctct tgctctgcaa gctgacgccc gtaactctga
     1081 tctgaccatt acccagcacg gcggcggtaa tggcgcagat gtgggccaag gttctgatga
     1141 cagctcaatc gatctgactc agcgtggttt cggcaacagc gctactcttg atcagtggaa
     1201 tggtaaagat tctactatga ctgttaaaca gttcggtggc ggtaacggtg ctgctgttga
     1261 ccagactgca tctaactcca gcgttaacgt cactcaggtt ggctttggta acaacgcgac
     1321 cgctcatcag tactaataca tcatcagtat gaaagaaaca gggcgcaagc cctgtttttt
     1381 tcgggagaag aatatgaatg cgttattact ccttgcggca ctttccagtc agataacctt
     1441 taatacgacc cagcaagggg atatgtacac cattattcct gaagtcactc ttactcaatc
     1501 ttgtctgtgc agagtacaaa tattgtccct gcgcgaaggc agttcagggc aaagtcagac
     1561 gaagcaagaa aagacccttt cattgcctgc taatcaactc attgctttga cgaagttgag
     1621 tttaaatatt tccccggacg atcgggtgaa aatagttgtt actgtttctg atggacagtc
     1681 acttcattta tcacaacaat ggccgtcctc ttcagaaaag tcttaatttg ttgaaatatc
     1741 gagcataaga tgaacctgga gagaatggtc tgctgcgaat cagccaacct gaaagtatgg
     1801 ataacacaac cctcaaggat gactaatcat tgaggaaata gaataaatgt tcagaccttt
     1861 tttaaactct cttatgctcg gcagtttgtt ttttcctttt attgccattg caggaagcac
     1921 cgcgcaaggg ggcgtgatcc attttaatgg ccaaattgtg gaaccggcat gtgacgtcag
     1981 cacccagtca tcacccgtag aaatgaactg cccacagaat ggttctgttc cgcgtaaaac
     2041 ctattccagc aaagcgttaa tgagcggcaa tgttaaaaac gcgcaaatag cctcagtaaa
     2101 agtgcagtat cttgataaac agaaaaagct ggctgtaatg aacatcgaat ataactaagt
     2161 ttctgaacaa ctcacgttgc tgagcagaaa aatgcgattt aaccaaaaag cctgctgtac
     2221 acttaggaaa caagaaggtg taaggaggcg ttatgaaaac gcgtattcat gttgtgcagg
     2281 gtgatattac caaactggcc gttgatgtga ttgtgaatgc ggctaatccg tcattaatgg
     2341 gaggcggcgg cgtcgatggg gccattcatc gcgcagcggg tccggccctg ctggatgcct
     2401 gtttaaaagt caggcagcag cagggcgatt gccctacggg gcatgccgtt attacgcttg
     2461 caggcgatct tcccgctaaa gccgtagtgc acaccgtcgg gccagtctgg cgtggtggtg
     2521 aacaaaacga agaccagctt ttgcaggatg cctatctcaa tagcctacga ctggtggcgg
     2581 caaacagcta tacgtcagtg gcttttcctg caatcagtac tggggtttat ggttaccccc
     2641 gtgcggcagc ggctgaaatc gcagtaaaaa ccgtttcaga atttattacc cgtcacgctt
     2701 tacccgaaca ggtatacttt gtctgttatg atgaagaaaa cgcccacctc tacgaaagac
     2761 tccttaccca acaaggagat gaatgatttg cctcagctgg cgagcgcggt gctgccactg
     2821 tgttcgcaac atcccggtca gtgtggactt tttcctctgg agaaaagtct ggatgcgttt
     2881 gccgcccggt atcgtctggc tgaaatggca gagcatacac tggatgttca gtattacatc
     2941 tggcaggacg atatgtcggg tcggttactg ttttccgccc tgttagccgc agcaaagcgt
     3001 ggcgttcgcg tacgtttgtt gctggacgac aacaatacgc ccggacttga taacatttta
     3061 cgcttgcttg acagccatcc acgcattgaa gtccggcttt ttaatccttt ctcgtttcgc
     3121 ttgctacgtc cgcttggtta tatcaccgac ttttcccgtc tcaatcgccg tatgcacaat
     3181 aaaagtttca ctgtcgatgg cgtggtgacg ctggtgggag gacgaaatat tggtgatgcc
     3241 tattttggtg caggggaaga gccacttttt tcggatttag atgtcatggc aataggaccc
     3301 gtggtagagg acgttgccga tgatttcgcc cgctattggt attgcaaatc ggtttccccc
     3361 ttacagcagg tgctggatgt cccggaaggt gaaatggcgg atcgcatcga gttaccggca
     3421 tcctggcatg acgatgccat gacgcatcgt tatttacgca aaatggaatc cagtccgttt
     3481 ataaatcatc tggttgatgg aacgttgtcg cttatctggg cgaaaacacg tttattaagt
     3541 gatgatccgg cgaaagggga gggcaaggca aaacggcatt cactgttacc gcagcgcctg
     3601 ttcgatatca tgggctcacc cagtgaacgc atcgatatta tctcttccta ttttgtaccg
     3661 acacgtgcag gtgtggcgca actcttacgg atggtgagaa aaggggtaaa gattgcgatc
     3721 ctaaccaatt ctcttgccgc taacgatgtt gctgtcgtcc atgcaggata cgcgcgctgg
     3781 cgcaaaaaat tgctccgcta tggggtggaa ttatatgaac tcaagccgac gcgtgaacaa
     3841 agtagtacgt tacacgatcg cggcataacc ggtaattcag gggccagcct gcatgctaaa
     3901 acctttagta tcgatggtaa aacggtgttt atcggctctt tcaatttcga tccgcgttca
     3961 acgttgctca atactgaaat gggcttcgtg atagagagcg aaacgctggc acagttaatt
     4021 gataaacgct ttattcagag ccagtatgat gcggcctggc agctccgtct ggacaggtgg
     4081 ggacggatca actgggttga tcgtcattca aagaaagaga ttattctcaa aaaagaaccc
     4141 gccaccagtt tctggaagcg ggttatggtc aaactggcgt cgatattgcc cgtggaatgg
     4201 ttattgtaaa cgcggcttaa cgggctggtg ctttatcgtt ctcacgctta acaaccggtt
     4261 taccagagaa caaaaacttc aataatggga tgcgtaaatg aatttcatac agaattatcg
     4321 caatccctac tacgaatatc aggccacaga gaaaaccaag ccagttggag gtgatgtgtg
     4381 gtgtaatgta tgcgccgaaa aacagcgtta acgggtggtg aaccagatag ataaacagcg
     4441 atgcgttaac aaaataagtc acccgcgctg actggaagtt aagcaaacgg tggccaaagg
     4501 agaagaccac attcaccatc cacagaccga ggaccatagt gatcaccgac tcggtttcat
     4561 acatccaggc atcgccactg ccatagcgct ggttgagtaa ataagcgaca aacgccaatg
     4621 ctgctgcaag ggtacagcca cgagacggcg tggtaaacaa ggctttaaga tgagggaaaa
     4681 tgaaagccag tgcgccgagg ataaagaacg gcaaataaaa taacgtttgc atgacaataa
     4741 aattgaacat gccgttactc agaatgggtg gatacacaat aaaaatcgtt cttcttatta
     4801 ccgcataacc gatgccgagg cacaaaaaaa tcaccgataa ttttaccatc gagaattttt
     4861 tattcgtttt atcagaattt tctaaattat ttctgatgcg tttaaatatc catacgcaca
     4921 gcgtcgtcat gaccactaac accagtaaaa accacaggtg tgatattaat tcccaggcca
     4981 acgtattata tttgtcatac aatgacagcc caggccaact ttccgctttc cctttgacgt
     5041 attgcagcat aataaattgc ggcaatgtca gtaaggggat ggctgttaac atcgggatcc
     5101 ctacacgttc gacacgtact ttccaccatt ttttcaaggg atagcgtaaa aaaagcatgt
     5161 aggaaaagta cccggatata acgaaaaaca cctgcatgcg gaacgagtgg atgaagtcat
     5221 taaaaagggt cagccacaat gacggttcgg cgctattcac atgccatgta tggctcgaat
     5281 agattaaaga aatatgaaaa gggatcccta acaacatcag ccaggcgcgg atggagtcga
     5341 ggaaatattc acgttgcgcg ggtactggat tcatatatgg ttaactaatc tcggattttt
     5401 cgtcttatcc ctgtcgggtt atgcctttag gcttgttgcc atagcgacac cgacctgacc
     5461 gcgccaggcg caggcttcaa ggtttttatg catagcatca tcgctaccac taaccagaat
     5521 ggaagcgtca gtaagacggt tgataaataa atttgctggc aaaccctaca cgaagtcgat
     5581 gcttctgtct ttaggagaag cacggaaagt gaaaacggtt gcaatcaggt gcttaatcca
     5641 tgagccagtg tgctgaacga taccgggatt ctgttgtcgg aatggctggt tatccattaa
     5701 aatagatcgg atcgatataa gcacacaaag ggggaagtgc ttactaatta tgaaacataa
     5761 actacaaatg atgaaaatgc gttggttgag tgctgcagta atgttaaccc tgtatacatc
     5821 ttcaagctgg gctttcagta ttgatgatgt cgcaaagcaa gctcaatcct tagccgggaa
     5881 aggctatgag gcgcccaaaa gcaacttgcc ctccgttttc cgcgatatga aatacgcgga
     5941 ctatcagcag atccagttta atcatgacaa agcgtactgg aacaatctga agaccccatt
     6001 caaactcgag ttctaccatc agggtatgta cttcgatacc ccggtcaaaa taaatgaagt
     6061 gactgccacc gcagtcaaac gaatcaaata cagcccggat tatttcactt tcggcgatgt
     6121 tcagcatgac aaagacacgg taaaagacct tggttttgcc ggtttcaaag tgctttaccc
     6181 gatcaacagc aaagataaaa acgatgaaat cgtcagcatg ctcggggcca gctatttccg
     6241 cgtgattggt gcaggtcagg tttatggcct ttctgcacgc ggcctggcaa ttgataccgc
     6301 cttgccatcg ggtgaagaat ttccacgctt caaagagttc tggatcgagc gtccaaaacc
     6361 gactgataaa cgtttaacca tttatgcatt gcttgactcg ccgcgcgcga caggtgctta
     6421 caaattcgta gttatgccag gacgtgacac ggttgtggat gtgcagtcga aaatctatct
     6481 gcgcgataaa gtcggcaaac tgggggttgc accgttaacc agtatgttcc tgtttgggcc
     6541 gaaccaaccg tcgcctgcaa ataactatcg tccggagttg cacgactcta acggtctgtc
     6601 tatccatgct ggtaatggcg aatggatctg gcgtccgttg aataacccga aacatttagc
     6661 ggtcagcagc ttctcgatgg aaaacccgca aggcttcggt ctattgcagc gtggtcgtga
     6721 tttctcccgc tttgaagatc tcgatgatcg ttacgatctt cgtccaagcg catgggtgac
     6781 tccgaaaggg gagtggggca aaggcagcgt tgagctggtg gaaattccaa ccaacgatga
     6841 aaccaacgat aacatcgtcg cttactggac gccggatcag ttgccggagc cgggtaaaga
     6901 gatgaacttt aaatacacca tcaccttcag ccgtgatgaa gacaaactgc atgcgccaga
     6961 taacgcatgg gtgcaacaaa cgcgtcgttc aacgggggat gtgaagcagt cgaacctgat
     7021 tcgccagcct gacggtacta tcgcctttgt ggtcgatttt accggcgctg agatgaaaaa
     7081 actgccagag gataccccgg tcacagcgca aaccagcatt ggtgataatg gtgagatagt
     7141 tgaaagcacg gtgcgctata acccggttac caaaggttgg cgtctggtga tgcgtgtgaa
     7201 agtgaaagat gccaagaaaa ccactgaaat gcgtgctgcg ctggtgaatg ccgatcagac
     7261 gttgagtgaa acctggagct accagttacc tgccaatgaa taagacaact gagtacattg
     7321 acgcaatgcc catcgccgca agcgagaaag cggcattgcc gaagactgat atccgcgccg
     7381 ttcatcaggc gctggatgcc gaacaccgca cctgggcgcg ggaggatgac tccccgcaag
     7441 gctcggtaaa ggcgcgtctg gaacaagcct ggccagattc acttgctgat ggacagttaa
     7501 tcaaagacga cgaagggcgc gatcagctaa aggcgatgcc agaagcaaaa cgctcctcga
     7561 tgtttcccga cccgtggcgt accaacccgg taggccgttt ctgggatcgc ctgcgtggac
     7621 gcgatgtgac gccgcgctat ctggctcgtt tgaccaaaga agagcaggag agcgagcaaa
     7681 agtggcgtac tgtcggtacc atccgccgtt acattctgtt gatcctgacg ctcgcgcaaa
     7741 ctgttgtcgc gacctggtat atgaagacca ttcttcctta tcaggggtgg gcgctgatta
     7801 atcctatgga tatggttggt caggatgtgt gggtttcctt tatgcagctt ctgccttata
     7861 tgctgcaaac cggtatcctg atcctctttg cggtactgtt ctgttgggtg tccgccggat
     7921 tctggacggc gttgatgggc ttcctgcaac tgcttattgg tcgcgataaa tacagtatat
     7981 ctgcgtcaac agttggcgat gaaccattaa acccggagca tcgcacggcg ttgatcatgc
     8041 ctatctgtaa cgaagacgtg aaccgtgttt ttgctggctt gcgtgcaacg tgggaatcag
     8101 taaaagccac cgggaatgcc aaacactttg atgtctacat tctgagtgac agttataacc
     8161 cggatatctg cgtcgcagag caaaaagcct ggatggagct tatcgctgaa gtcggtggcg
     8221 aaggtcagat tttctatcgc cgccgccgcc gtcgcgtgaa gcgtaaaagc ggtaatatcg
     8281 atgacttctg ccgtcgttgg ggcagccagt acagctacat ggtggtgctg gatgctgact
     8341 cggtaatgac cggtgattgt ttgtgcggcc tggtgcgcct gatggaagcc aacccgaacg
     8401 ccgggatcat tcagtcgtca ccgaaagcgt ccggcatgga tacgctgtat gcgcgctgcc
     8461 agcagttcgc gacccgcgtg tatgggccac tgtttacagc cggtttgcac ttctggcaac
     8521 ttggcgagtc gcactactgg gggcataacg cgattatccg cgtgaaaccg tttatcgagc
     8581 actgtgcact ggctccgctg ccgggcgaag gttcttttgc cggttcaatc ctgtcacatg
     8641 acttcgtgga agcggcgttg atgcgccgtg caggttgggg ggtctggatt gcttacgatc
     8701 tcccgggttc ttatgaagaa ttgccgccta acttgcttga tgagctaaaa cgtgaccgcc
     8761 gctggtgcca cggtaacctg atgaacttcc gtctgttcct ggtgaagggt atgcacccgg
     8821 ttcaccgtgc ggtgttcctg acgggcgtga tgtcttatct ctccgctccg ctgtggttta
     8881 tgttcctcgc gctctctact gcattgcagg tagtacatgc gttgaccgaa ccgcaatact
     8941 tcctgcaacc acggcagttg ttcccggtat ggccgcagtg gcgtcctgag ctggcgattg
     9001 cactttttgc ttcgaccatg gtgctgttgt tcctgccgaa gctattgagc attttgctta
     9061 tctggtgcaa aggaacgaaa gaatacggcg gcttctggcg cgttacatta tcgttgctgc
     9121 tggaagtgct gttttccgtg ttgctggctc cggtacgcat gttgttccat acagtcttcg
     9181 tcgtcagcgc gttccttggc tgggaagtgg tgtggaattc accgcagcgt gatgatgact
     9241 ccacttcctg gggcgaagcg ttcaaacgcc acggctcaca gctgctgtta gggttagtgt
     9301 gggcagttgg tatggcgtgg ttggatctgc gttttctgtt ctggctggca ccgattgtct
     9361 tctcgttgat cctgtcaccg tttgtttcgg tgatttccag ccgtgccacc gttggtctgc
     9421 gaaccaaacg ctggaaactg ttcctgatcc cggaagagta ttcaccgccg caggtgctgg
     9481 ttgataccga tcggttcctt gagatgaatc gtcaacgctc ccttgatgat ggttttatgc
     9541 acgcggtgtt taacccgtca tttaacgctc tggcaaccgc aatggcgacc gcgcgtcacc
     9601 gcgccagtaa ggtgctggaa atcgcccgtg accgccacgt tgaacaggcg ctgaacgaga
     9661 cgccagagaa gctgaatcgc gatcgtcgcc tggtgctgct aagcgatccg gtgacgatgg
     9721 cccgtctgca tttccgcgtc tggaattccc cggagagata ttcttcatgg gtgagttatt
     9781 acgaagggat aaagctcaat ccactggcat tgcgtaaacc ggatgcggct tcgcaataaa
     9841 aacgtagttg cctgatgcgc tacgcttatc aggcctacat cgttcctgca atttattgat
     9901 tttgcaagat tttgtaggtc gggtaaagcg ttcaccccgc atccggcaaa aacaacgagc
     9961 cgataaaaat accggcgtta tgccggtatt ttttacggaa gaggtatcaa atgcgtttaa
    10021 ttgtgatgag cataatggtg accttgctga gtggctgtgg cagcatcatt agtcgcacta
    10081 taccggggca ggggcatggc aaccaatatt atcccggtgt gcaatgggac gtgcgtgact
    10141 ccgcctggcg ttacgtcacg atccttgatc tgccattctc cctggttttc gatactttac
    10201 tgctgccgat cgacgtacat catggcccgt atgagtgatt aacgttcatc ccactcatca
    10261 gctgcagtgc gtccttcctc ggtatccagc ggtggctcaa gctgaaattc cccctcgtcc
    10321 cattcatgta atgtattctc atcttgccac tcctggcgga tctctatctc atcatagtcg
    10381 ccgtcaaaga cactttgcgc agcttcaccg ctaagcatgg gtaaacattc accttcttcc
    10441 ccttcgtcgg caaaaaactc aacttgccac atgatgtcgc cgtcctgcaa aacgtatttt
    10501 tgggcattga actgttgcac attcgcgttt tcggcgtcga tgccggggtt gtctgcaaga
    10561 aattcttcgc gggcagcgtc aatggcttct tcaagcgttg cgtacatggt catcacgatt
    10621 ctccctttga gttgatgagg tttcagggaa aaggatagct gattctccgc ttttgcaagt
    10681 atgaaaggcg aaaaatcagt tcgatatctg ggggattcga cgacgacgta gactgttcca
    10741 tgaatagact gcgttgaata acactacgcc agcggtgacg agaaataccg ccctgaaacc
    10801 gtagttcgct gaaatcgctg ctcccatcaa tggtccggta acgttgccaa tatcacgaaa
    10861 cgattggtta tagctgaaga tacgcccggc gatctggttg ctcgagttgt aaaccaacag
    10921 tgtctgtacg gcggggagta atgcaccatc ggcggcaccg agcaaaaaac gtaaaatccc
    10981 aagttgtaat ggcgtctgaa cgtaagacat tgggatcaac agcagtacag aaaagatcag
    11041 cgccgtaatc aggatctttt cggggccgat tcgatcgcca agtttgccga gtcgtggtgc
    11101 acttagcaga gccgccacgc ctggcaccga ggcgatcatg ccactgataa aagcgacgtt
    11161 actgacgtta cccgccagtt cgcggacata cagcgtcaga atgggggcaa ttgagcccgt
    11221 cgccacctgg atgattaacg tggtgacaaa caggctgagt accagtttcg ggtttttaag
    11281 tgatgtcacc acttcccgca tatgcagcat ctcttttttg ctgaccggct ggaatttttc
    11341 tctgatgcaa aacagggtga cgaaaaagca gagtatgagc acactggcgg taataaagaa
    11401 taccggacgt aagccgtagc tatcggcgag caaaccgcca gccattgggc cgagcaacgc
    11461 gccgctaacg ccgcctgtgg agagcgtacc cagcgcccag ccgcttttat tacgcggtac
    11521 ttgtgtggcg ataagagcat tagcgttggg gacaaatccg ccaagtaaac caagaagcgc
    11581 ccgcaggatc aaaaactgcc agatattttg tgccagtccc atcaacacca tcacgatgcc
    11641 catgccgaga gcagagcgta atagcatgag ttttcggcct ttacggtcgg cgagtccacc
    11701 ccaaaacggt gaggcgatgg ccgaaaataa aaatgtaatg ctgaagacaa taccggacca
    11761 catattcagg gcggagtggc cggtaacgcc aagctgctca acgtagaggg gtaagaaggg
    11821 cattaccagg ctgaaggcgg caccggtaag aaaacagcct agccaggcga cgatcaggtt
    11881 tcgtttccag tttatagggg tgtcattttc acagggtgac atagcaatcc gctgttggtg
    11941 cgccaggcgc ggtgaacata agaagaaaag ataagcacac taattatgcg cccgacttcc
    12001 agggggcgca atccagagag cttttatcgc taaatcaggg ggattttctg tggtaatgcc
    12061 ggatgccatt ctgaagcatc cagcatggaa gatttaatag cgtgaaggaa cgccttccgg
    12121 gcgtgtttta aagcgacggt gtaaccacat atactgctct ggtgccatca tgatgcattt
    12181 ttcgaccact ttgttcatcc acgcggcggt agtttcggca tcatccagcg gcggagaaca
    12241 ctctggcggc agtataatca actgataccc tttgccatct ggcttacggc gtggaacgaa
    12301 gggcaccaga catgcgccgg acatccgtgc cagcatccag gttccggtcg tggtcgcagc
    12361 ctgctcaacg gcaaacaacg ggacgaaaac gcttgagcgc gggccgtaat catgatccgg
    12421 tgcgtaccag accacttcgc cttttttcag ggctttaatc atgcctttta aatctttgcg
    12481 gtcgagcatc gatttatttg agcgcaaacg gccccaggtt tgtagccagt caatcagtgg
    12541 attatcgttc gggcggtaaa cgccaatccc cggttcctgc ataccaaact gccgcgcacc
    12601 cagctccagt gtcagaaaat ggatgccaac taacaggatg ccgcgttttt gcgcctgcac
    12661 gtcacgaatg tgttccatgc cgatcacttc cgtccagcgg gcgattcggc ggtccggcca
    12721 gaaccacgcc atgccggttt ccatcaggcc catgccaacg gattcgaaat tcttcaccac
    12781 cattttacgg cgttcttgtt cgctcatttc cgggaagcac agttccaggt tgcgatgcac
    12841 aatttttgcg cgtcgtttca taaaacgtaa cgccagtttt cctaatccac aaccgaggcg
    12901 gtagataacc gggtagggca attgcacgac caaccaaagt acgccaatac ccaaccaggt
    12961 taaccaataa cgcggatgaa gcagtgcggt ggagaacttg ggtagattcg tcatatcaat
    13021 cctgtttttc aacctatacg ggcaattgta tgtattgtcg cattttttcg tccgcaacca
    13081 aaatttgtga ctgaagactg ggcgaaattg ccgcgcttgt aaataacaaa taatttttaa
    13141 tgctaaaatg tagcgtaaaa tgtgtggatg ttaattatcg ataattgcta tatcatgccg
    13201 cggattttta ctttcccatc tcgcaggaac cgtacaccat gccagtgtta cacaaccgca
    13261 tttccaacga cgcgttaaaa gccaaaatgt tggctgagag cgaaccgcga accaccattt
    13321 cgttttataa gtatttccac attgccgatc ctaaggtgac ccgtgacgct ttatatcaac
    13381 tgtttaccgc gctgaatgtt tttgggcgag tgtatctggc gcatgagggc attaacgcgc
    13441 aaatcagcgt acctgcgagc aatgttgaaa catttcgcgc gcagctctat gccttcgacc
    13501 cggctttaga gggtttacgc ctgaatatcg cgttggatga tgacgggaaa tccttctggg
    13561 tactgcgtat gaaggtacgc gatcgcattg ttgccgacgg tattgacgat cctctctttg
    13621 atgccagcaa tgttggtgag tatctgcaag cggcggaagt gaacgccatg cttgacgatc
    13681 ccgatgcatt gtttatcgac atgcgtaacc actatgagta tgaagtgggg cactttgaaa
    13741 acgcgctgga aataccggca gataccttcc gtgagcagct gccaaaagca gttgagatga
    13801 tgcaggcaca taaagataaa aaaatcgtca tgtactgcac cggcggcatt cgttgtgaaa
    13861 aggccagtgc ctggatgaaa cataacggat tcaacaaagt ctggcatatc gagggcggaa
    13921 ttattgaata cgcccgtaag gcgcgcgagc agggcttgcc ggtgcgtttt attggcaaaa
    13981 attttgtttt tgacgagcgg atgggcgaac gtatatctga tgagattatc gcgcactgcc
    14041 accagtgcgg tgcgccgtgc gacagccata ccaactgtaa aaatgatggc tgccatctgt
    14101 tgtttattca gtgtccagta tgtgcggaaa aatacaaagg ttgttgtagt gagatttgct
    14161 gcgaagaaag cgcgttaccg ccagaggaac agcgacgccg tcgggcagga cgtgaaaatg
    14221 gcaataagat ctttaataag tctcgtggac gtctgaatac aacactgggc attcctgaac
    14281 caacagaata aatatcattg ccggatgcgt gccatccggc aacatttcac tcttacttct
    14341 gctgtacgcc ttccactgaa ataatcagat ccacttcctg agacgctgga cctaaatctg
    14401 tcttgatatt gaagtctttg agctcgattt tgccttcggc ctcaaagcct gcacgtttac
    14461 cgccccacgg gtcgtcaccc tgaccaatta atttcgcttc cagtgtgacg ggcttggtta
    14521 caccattcag cgtcagatcg ccggtaatat ccagttcgtc accgtctttc ttcacgctgg
    14581 tggaggtgaa tgttgcctgt ggatattttg cggtattgag gaaatccgca ctgcgaagat
    14641 gtttatcgcg ttcggcgtga ttagtatcga cgctggtggt gttaattgtc acattcactt
    14701 tatcggcagc tggatttttt tcatcaaagg taaaagtacc gtcgaaatct ttgaaggtgc
    14761 catataacca gctatagcca agatgctgga tgcggaagtt aacaaaggcg tgctgacctt
    14821 ctttgtcaat tttgtaatcg gcggcaaccg ctgaaccggc agagaacatc aaggaagcga
    14881 aggttaaacc aagcaggctt tttttcattt tttatactcc atagtcagat gacgactttc
    14941 ccagcatgcg cttcagagtg tcgtctttat cgatgaaatg atgcttcagg gccataaatc
    15001 cgtgcataac ggacagtact acgacgctcc acgcgagcca aaaatgcagg gcaccggcaa
    15061 agtcagcctg tgcgccagcg tcggcgaggg tcgcggggac gtcaaaccag ccaaaaacgc
    15121 tgatcggttt accatcggca gttgagatca gatagccgct gatgccgatg gcgaacagca
    15181 ataggtacaa tgcgagatgt ccagcttttg ctgcaagacg agtcatgggc gaataactcg
    15241 gcagcggacc cggcggtgga gatatgacac gccacagaac gcgaataacc agccccatca
    15301 ttaacaaaat accgatgctt ttatgcagtt cgggtgcttt gtgataccag ccatcgtaat
    15361 aactgagcgt gaccatccac agacctaaag caaacatgcc gtagacaata attgcgctta
    15421 tccaatgtaa ggccgcagat ataacaccat agcgttcagg ggtatttgtg aatgacataa
    15481 gtacatccaa aacgaatttg cgagagaaag aaaatggcgt gtcggagaat gaattg
//
LOCUS       DAZGJT010000102        15267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZGJT010000102 DAZGJT010000000
VERSION     DAZGJT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.102"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..121
                     /gene="yecU"
                     /locus_tag="ACHKFK_003754"
     CDS             <1..121
                     /gene="yecU"
                     /locus_tag="ACHKFK_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO9053999.1"
                     /translation="TLKKQPLLFLAKLMVYSAALTFFTANFHCNMTRKINEYA"
     gene            108..332
                     /gene="yecF"
                     /locus_tag="ACHKFK_003755"
     CDS             108..332
                     /gene="yecF"
                     /locus_tag="ACHKFK_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO9054000.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(400..1122)
                     /gene="sdiA"
                     /locus_tag="ACHKFK_003756"
     CDS             complement(400..1122)
                     /gene="sdiA"
                     /locus_tag="ACHKFK_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO9054001.1"
                     /translation="MQDTDFFSWLRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(1352..2104)
                     /gene="tcyN"
                     /locus_tag="ACHKFK_003757"
     CDS             complement(1352..2104)
                     /gene="tcyN"
                     /locus_tag="ACHKFK_003757"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO9054002.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGVAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(2101..2769)
                     /gene="tcyL"
                     /locus_tag="ACHKFK_003758"
     CDS             complement(2101..2769)
                     /gene="tcyL"
                     /locus_tag="ACHKFK_003758"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO9054003.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(2784..3770)
                     /gene="dcyD"
                     /locus_tag="ACHKFK_003759"
     CDS             complement(2784..3770)
                     /gene="dcyD"
                     /locus_tag="ACHKFK_003759"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO9054004.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNTQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(3875..4675)
                     /gene="tcyJ"
                     /locus_tag="ACHKFK_003760"
     CDS             complement(3875..4675)
                     /gene="tcyJ"
                     /locus_tag="ACHKFK_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9054005.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(4763..5314)
                     /gene="fliZ"
                     /locus_tag="ACHKFK_003761"
     CDS             complement(4763..5314)
                     /gene="fliZ"
                     /locus_tag="ACHKFK_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO9054006.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPSTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSQPASDIY"
     gene            complement(5360..6079)
                     /gene="fliA"
                     /locus_tag="ACHKFK_003762"
     CDS             complement(5360..6079)
                     /gene="fliA"
                     /locus_tag="ACHKFK_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO9054007.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(6399..8156)
                     /locus_tag="ACHKFK_003763"
     CDS             complement(6399..8156)
                     /locus_tag="ACHKFK_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFO9054008.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGETITIDL
                     KKIDSDTLGLNGFNVNGKGTITNKAATLSDLTSAGAKLNTTTGLYDLKTENTLLTTDA
                     AFDKLGNGDKVTVGGVDYTYNAKSGDFTTTKSTAGTGVDAAAQATDSAKKRDALAATL
                     HADVGKSVNGSYTTKDGTVSFETDSAGNITIGGSQAYVDDAGNLTTNNAGSAAKADMK
                     ALLKAASEGSDGASLTFNGTEYTIAKATPATTSPVAPLIPGGITYQATVSKDVVLSET
                     KAAAATSSITFNSGVLSKTIGFTAGESSDAAKSYVDDKGGITNVADYTVSYSVNKDNG
                     SVTVAGYASATDTNKDYAPAIGTAVNVNSAGKITTETTSAGSATTNPLAALDDAISSI
                     DKFRSSLGAIQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGN
                     SVLAKANQVPQQVLSLLQG"
     gene            8422..9828
                     /gene="fliD"
                     /locus_tag="ACHKFK_003764"
     CDS             8422..9828
                     /gene="fliD"
                     /locus_tag="ACHKFK_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO9054009.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQNSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSNYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            9853..10263
                     /gene="fliS"
                     /locus_tag="ACHKFK_003765"
     CDS             9853..10263
                     /gene="fliS"
                     /locus_tag="ACHKFK_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO9054010.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEKSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPI"
     gene            10263..10628
                     /gene="fliT"
                     /locus_tag="ACHKFK_003766"
     CDS             10263..10628
                     /gene="fliT"
                     /locus_tag="ACHKFK_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO9054011.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            10706..12193
                     /gene="amyA"
                     /locus_tag="ACHKFK_003767"
     CDS             10706..12193
                     /gene="amyA"
                     /locus_tag="ACHKFK_003767"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO9054012.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(12227..12640)
                     /gene="yedD"
                     /locus_tag="ACHKFK_003768"
     CDS             complement(12227..12640)
                     /gene="yedD"
                     /locus_tag="ACHKFK_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO9054013.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLTGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            12827..14032
                     /gene="yedE"
                     /locus_tag="ACHKFK_003769"
     CDS             12827..14032
                     /gene="yedE"
                     /locus_tag="ACHKFK_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO9054014.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQIAKEIA"
     gene            14029..14262
                     /gene="yedF"
                     /locus_tag="ACHKFK_003770"
     CDS             14029..14262
                     /gene="yedF"
                     /locus_tag="ACHKFK_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO9054015.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            14371..15038
                     /gene="yedK"
                     /locus_tag="ACHKFK_003771"
                     /pseudo
     CDS             14371..15038
                     /gene="yedK"
                     /locus_tag="ACHKFK_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
BASE COUNT         3830 a         3914 c         3712 g         3811 t
ORIGIN      
        1 cacgctcaaa aaacaaccac tgctattttt agcaaagctt atggtatact ccgccgcctt
       61 aacatttttc accgcaaatt ttcattgcaa catgacgagg aaaataaatg agtacgcctg
      121 atttttctac tgccgagaat aatcaagaac tggcaaatga agtctcctgc ctgaaagcga
      181 tgctgacgct gatgctgcag gcgatgggac aagctgacgc gggccgcgtg atgttaaaga
      241 tggaaaaaca gcttgcgctg atcgaagacg aaacccaggc tgcagtattt tccaaaacgg
      301 ttaagcaaat taaacaagcc taccgtcagt aatataaaac cggctgagag cgtgcctttc
      361 agccggtttt tgcatctggc acgcagaaca gaaaagagat caaattaagc cagtagcggc
      421 cgcgtaacag gcaacctgcg tcttattcgg tgcattgatt tttttctgca tgtttttctg
      481 atggaaattg accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt
      541 accttccgcc gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt
      601 catcactatt tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtagctg
      661 catttttaat tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa
      721 ggacaaaaag cccaacgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa
      781 accatgtgcg cgcgcagctt cccataacgg ctgtgcttcg ctgaataagt catcattcca
      841 cattaaatgg ccctgactaa agttttcagg gttcagcacc ggatcaattg cgagaaaatt
      901 ttttgcctga taataactaa cccacgactc agggtaattg gtatagaaag ccactttagg
      961 tcgcgtgaat ggtaccgggt ggcgaacaca taacgaatag taatcgtact ccagctgctg
     1021 agcctgaagc tctatttcat ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa
     1081 caacatcgtg cgaagccagc tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc
     1141 tgagagtgag tatcatttat aatgataatt caaactatca cataaataag atttatatat
     1201 aatttatatt attcaggcaa tgaattactt ttgcaagcca tcgcattctc ttatgttatt
     1261 aatgagttat gctgatttgt taagcagttt tatcacgttt gaaatggcgt cccgccccga
     1321 ctggtgaatc gtcggggctg attttttctt attattgcag cagaaacttc tccaggaact
     1381 ggcgggtgcg aggctgctga ggatcagcaa ataacgcttt tgctaccccc tgctcgacta
     1441 tccgcccctg gtccataaaa atcgcccggt ccgcaacatc ccgggcaaag ctcatttcgt
     1501 gcgtcacaat caccatcgtg cgcttttcct gcgccagctg acgaatggtg ttcaggactt
     1561 cgcccaccag ctctggatcc agcgccgacg ttggctcatc aaacaaaatc acttcaggac
     1621 gcattgccag agcacgcgca atcgcaacgc gctgctgttg accgccagac aaacgacgtg
     1681 gataactggt ttctttaccg gccagcccaa ctttcgccag cagctcgcga gcgcgcgccg
     1741 tggcctcttc tttcggttcg cctttgacga tcaccggccc ttcaataatg ttttccagca
     1801 ccgtacgatg cggaaacaga ttaaagttct ggaagacaaa cccgacgtgc tgacgcaact
     1861 ggcgaatcag cgatttttgc tgacttaatg aacgtgcagt atcaatagtg atatcgccaa
     1921 cggtgatggt tcccgcttcc ggttgttcca gcagatttat gctgcgtagc aacgtggttt
     1981 tgccggaacc actcggaccg ataatagcca ctacttcgcc aggcttcacc tcaaggtcga
     2041 taccatgcag caccgtctga ccgtggaatt ttttcaccag gttcttaact tcaatggcac
     2101 tcatttgggt tccctctcct ggcgattaag ttgattctca aaatggttct gcaacgtcga
     2161 taacacagtc gccataatcc agtagatgag cgaagccgcc aggtacatgg tgaacacctc
     2221 cagcgtgcgc gaggtaatca actgcgcctg acggaacagc tccggcacct ggattgtcgc
     2281 ggccagcgac gtatctttta ccaggctgat aaagctgttc gacagcggcg gcaacgccac
     2341 ccgcgccgcc tgtggcaaaa tcgcgcgacg catggtctgc catggcgtca taccaatact
     2401 ggctgcagct tcccactgac ctttatcaat agaagaaatg gcggcgcgca gcgtttcggc
     2461 agcataggcg gcagtgttca gcgacagacc aatcattgct gacggaatgg gatctaattc
     2521 gataccaaac tgtggcagtc cgtaatagat cataaacagt tgtgcgatga gcggcgtacc
     2581 acgaaaaata gagatataaa aacgcgccaa ccagcgcacg ggccagattg gcgacaggcg
     2641 catcagcgcg agaataaacc cgagcagtaa gccaaaaaac atgccgccga tactgagttg
     2701 cagcgtatac ccagcccctt tcagcaggaa cggcaaggaa tcaataacca gttgtatact
     2761 ttcttgcatg agcgttcccg gagctaaacg tggggatgat aggcgaacag cgcaggcgca
     2821 ccgccggtat gaataaacaa aatcggccct tcatctttga agcgtttctg actgataccg
     2881 tcaatcagcc ccgccatcgc ttttccggta tacacaggat caagcagaat gccttccagc
     2941 cgcgccagca atttcactgc ttccatgcct tcgtcgttcg gcacgccgta gccaggtgca
     3001 aaatagtcat cccagagtaa aatttccgct gatgcggtca gctccagttc tttcgcaatc
     3061 gcctgttgga ggttaaccac tttcggcaat tgatcggcaa cagaacgcga cacagtcacg
     3121 ccaatcagtt cgctttcagg catcaggtgt tccagcccaa cagccagtcc ggcgtgagtt
     3181 ccggcactgc ccgatgcgac caccaccgac gaaatattaa ccgccccttc acactgttgc
     3241 gcgatttcca gcgcactctc cacataaccc agcgcgccca aagcattaga accgccaacc
     3301 ggaatgacat acgggcgaaa gccttgtgct tcgactcgcg tcgccagctc ttccagttgg
     3361 gtattgggat cggtcagtgc gtcgcacatt tcaatctggg tattgaacag atccagcaac
     3421 aaacgattgc cgttggttaa atagttttcc gcggttgtac caataggatt ttccagcagc
     3481 gccacgcagt gcagaccgag tttcgcagcg actgcggcag tctggcgcac atggttagac
     3541 tggatcgccc cggcagtaat cagcgtatcg gcaccttcac gcagagcatc tgccgcgaga
     3601 aattccagct tacgtaattt attgccgccc attgccatgg gagtgacgtc atcccgtttg
     3661 atgaaaattt cccgtccaag ataatcagaa aagcgcggca gatattcgag cggcgttggc
     3721 gcgccgataa actccagccg tggaaaacgg gttaaattat gcagtggcat aacagcctcc
     3781 gatgtgtgtt gttgtgattt tcttattatg cacgctgaaa acgcgtaaat aaaaaaggcg
     3841 ctagtgaaag cgcccttttt tgtcattatg ctgattattt ggtcacatca gcaccaaacc
     3901 atttttcgga aagcgcttgc agagtgccgt ctttttgcat ttccgcaatt gcatcattca
     3961 ctgctttcag caggtcttca tttcctttac gcaacgccac gccagactcc tgacgggaga
     4021 atgcttcacc ggttactgcc agcgtatcgt tggttttctt caccagatcc agcgccgcca
     4081 gacgatcaac gaggatcgca tcgatacggc ctacgcgcag atcctgatat ttggtcgggt
     4141 catcatcata ggtacgcaca tccacgccct gaacattctg acgcagccac tcttcatagt
     4201 tggtgcccag accgacaccc acttttttgc ctttcagatc atcagccgtt ttaatggtgc
     4261 cttcgttacc ttttttcacc agcgcctgaa taccggaaat ggtgtacggg gtagagaaat
     4321 cgtatttttt cttgcgctcg tcagaaatgg tgacctggtt aatcaccaca tcaatgcgtt
     4381 tagagtccag cgacgccagc ataccgtccc atttagtcgg ttttagtgac gcctcaacgc
     4441 caagatgttt tgccagctgt tgggcaaatt ccacttcaaa accggttaat ttgccgtcat
     4501 ccccctgaaa actgaacggc ggataagttc cttccagccc taccagcagc gtgccgcgct
     4561 ctttgacttt attaagcaga ccttcatctg caaaactttt aacgctcata cccgcaacca
     4621 gcgccacggc catcacaccc atcaatgcct gacgtcccag atgtgctaat ttcatattca
     4681 ccccgaatgt tgttatgtct gtttgcagtg tagagccatt gtttgtaaac acaaaaacaa
     4741 ctccgctaca tcttattctt atttaatata tatcagaagc aggttggctg gaggatttct
     4801 gcacaagtcc ggtacacgtt tgacgctgat agtgttgata cttccgtaac gcgatgcggt
     4861 aattgttggt gctggtggag ggcagccacg gggcgaggat ttcatcaagg aaaccgtcct
     4921 gcagctggtc gagggaaata ttttgctcgt gtagatgatt ccccaaacgg cgcagacgaa
     4981 cgacatattc acgcaccgta ccagggctca tttcagtttg ctcaaaaaga aattgcttaa
     5041 agccgataat atcgaaaaaa tcactctgcg ttttgcaatg taaatcaccg caaaatcggc
     5101 acaatgccgc ccatgattgt tgttccactt gccagccatt ttcatcaagc agcgtgtcca
     5161 ggcgggaaat ctcgatttta ttcactattt tgccgtcgcg aactaaggta atgcgatcga
     5221 gtaatttacg gcaatgcgcg caatgggtct ggctgtgttt aaagtcttta agatagcggc
     5281 ttaatggccg tcttttcagg tgctgcacca tcattaagaa ctcctggtag tcaaaattaa
     5341 agtgcggcat ttactgacgt tataacttac ccagtttagt gcgtaaccgt ttaatagcct
     5401 ggctgtgtaa ctgactgacc cgcgattccc cgacctccag caccgcacca atctctttga
     5461 gattcagctc ttcctgataa tagagggtta ataccagttt ttcgcgctcc ggcaacgttt
     5521 cgatggcttc catcacccgc tggcgcagat tactgtccag cagttgttgt agcgggtttt
     5581 ctcgctgatg atcatcagta accagttcga tgctatcgcc gtgctcttcg cgccactcat
     5641 cgtaggagaa gagctggctg ttattggtgt cgagcaacat ttggcgataa tcggcaatat
     5701 cgatccctaa acgttccgct acctcagttt ccgtggcgtt gcggccaagt tcctgctcca
     5761 gttgccctat tgcctgtgcc acttcacgcg cgttgcgtcg cacgctgcgc ggcacccagt
     5821 cacggctgcg aagttcatcc agcatagcgc cacgtatacg ctgcactgcg taagttgtaa
     5881 atgccgttcc ttgtagggcg tcatagcgtt cgacggcatt aagtaaccca atgccgcccg
     5941 cctgtagcag atcgtcaagt tccacgctcg cgggcagtcg aacctgcagg cgcaatgctt
     6001 cgtgacgcac cagcgggaca taacgctgcc acagcgagtg tttatccatt acaccttcag
     6061 cggtatagag tgaattcacg ataaacagcc ctgcgttata tgagttatcg gcatgattat
     6121 ccgtttctgc agggttttta atcgggcgat tagtgggtga aatgaggggt tatttggggg
     6181 ttaccggtaa attgcgggca gaaaaaaacc ccgccgttgg cggggaagca cgttgctgac
     6241 aaattacgct tcatgttgcc ggatgcggcg taacgcctta tccggcctac aaaaatctgc
     6301 aaattaaata aattgcaatt caccttgtag gcctgataag cacagcgcac caggcaattt
     6361 ggcgttgccg tcagtctcag ttaatcaggt tacggcgatt aaccctgcag cagagacaga
     6421 acctgctgcg gtacctggtt ggcttttgcc agcacggagt taccggcctg ctggataatc
     6481 tgcgctttcg acatgttgga cacttcggtc gcatagtcgg cgtcctgaat acgggactgc
     6541 gcttcagaca ggttggtagt ggtgttgttc aggttggtga ctgcggaatc cagacggttc
     6601 tggatagcac ccagggaaga acggaatttg tcgatggagc tgatagcgtc gtccagggca
     6661 gcaagcgggt tggtcgttgc agaaccagca ctggtagtct cagtagtgat tttacccgcg
     6721 gagttcacat ttacagcagt accaattgct ggagcataat ctttattggt atcagtcgct
     6781 gaagcatacc cggcaacagt cacagagccg ttatccttgt taacgctgta agagactgta
     6841 tagtcggcaa cgttagtaat accaccttta tcatccacat aagacttcgc agcatcactg
     6901 gattcacccg cggtaaaccc aatagttttg ctcagtacac cggaattaaa ggtaattgaa
     6961 gatgtcgcgg cagccgcttt ggtttcgctc aatactacat ctttactcac tgtagcctga
     7021 taagtaatcc caccagggat taacggagct actggagagg ttgtcgcagg agttgctttt
     7081 gcgatagtat attcagtgcc attgaatgtc agagaagcac cgtcactacc ttcgctcgcg
     7141 gctttaagca gcgctttcat atcagcttta gctgcgctac cagcgttgtt agtcgtcaag
     7201 ttgcctgcat cgtctacgta tgcctggctt ccaccgatgg tgatattacc tgctgaatcc
     7261 gtttcgaaag aaacagtacc atcttttgtg gtgtaagaac cattaacaga tttacccaca
     7321 tcagcatgaa gggtggcagc taacgcatca cgttttttag ctgaatcagt agcctgcgcc
     7381 gcggcgtcta cacccgtacc agcagtagat ttggtggtag taaaatcacc agatttagcg
     7441 ttgtaagtat aatctacgcc gccaacggtg actttatcgc cattccctaa tttatcgaat
     7501 gcagcatcgg tagttaacaa ggtattttcg gttttcagat cataaagacc tgtcgtggtg
     7561 tttaacttcg cgccagcaga agttaaatca cttaacgttg cagctttgtt ggtaatagta
     7621 cctttaccat ttacgttaaa accattcaga cccagagtat cagaatcgat tttcttcagg
     7681 tcgatagtga tagtttcacc gtcattcgca ccaacctgaa ttttcatcga accgtctttt
     7741 gccagtacgt tcacgccgtt gaactgggtc tggccggata cgcggtcaat ttcatcaaga
     7801 cgggatttga tttcgtcctg aatggagtcc agatccgaat cagagttagt tccggtagaa
     7861 gcctgaaccg tcagctcacg gatacgctgt aagttgttgt tgatttcaga cagcgcgcct
     7921 tcggtggtct gtgcaacaga aataccgtcg ttggcgttac gtgcagcctg agtcaggcct
     7981 ttaatgttag aagtaaaacg gttagcaatc gcctgacccg cggcgtcatc cttcgcgctg
     8041 ttaatacgca agccagaaga cagacgctcg atagaactcg acagcgcaga ctggttcttg
     8101 ttgatattat tttgagtgat cagcgagagg ctgttggtat taatgacttg tgccatgatt
     8161 cgttatccta tattgcaagt cgttgattac gttttgggtt tccacccgtc ggctcaatcg
     8221 ccgtcaaccc tgttatcgtc tgtcgtaaaa caacctttag aatttttttc acaaacagcc
     8281 attttttgtt agtcgacgaa atactctttt ctctgcccct tattcccgct attaaaaaaa
     8341 acaattaaac gtaaactttg cgcaattcag gccgataacc ccggtattcg ttttacgtgt
     8401 cgaaagataa aaggaaatcg catggcaagt atttcatcgc tgggagtcgg gtcaggtctg
     8461 gatttaagtt caatccttga tagcctcacc gccgcgcaaa aagcgacgct aacccctatt
     8521 tcaaatcagc aatcgtcgtt taccgctaaa cttagcgcct acggtacgct gaaaagcgcg
     8581 ctgacaactt tccagaccgc caatactgca ttgtctaaag ccgatctttt ttccgctacc
     8641 agcaccacca gcagcaccac cgcgttcagt gccaccaccg cgggtaacgc catcgccggg
     8701 aaatacacca tcagcgtcac ccatctggcg caggcgcaaa ccctgacaac gcgcaccacc
     8761 agagacgata cgaaaacggc gatcgccacc agcgacagta aactcaccat tcaacaaggc
     8821 ggcgacaaag atccgattac cattgatatc agcgcggcta actcgtcgtt aagcgggatc
     8881 cgtgatgcca tcaacaacgc aaaagcgggc gtaagcgcaa gcatcattaa cgtgggtaac
     8941 ggtgaatatc gtctgtcagt cacatcaaat gacaccggcc ttgacaatgc gatgacgctc
     9001 tcggttagcg gtgatgatgc gctacaaagt tttatgggct atgacgccag tgccagcagc
     9061 aacggcatgg aggtctcggt cgccgcccag aatgcacagc tgacggtcaa taacgtcgcc
     9121 attgagaaca gcagcaacac catcagcgac gcgctggaaa acatcaccct gaacctgaac
     9181 gatgtcacca caggcaacca gacgctgacc atcactcagg acacctccaa agcgcaaacg
     9241 gcgattaaag actgggtgaa tgcctataac tcgctaatag atacctttag cagcctgacc
     9301 aaatacaccg ccgtagatgc gggagctgat agccagaatt ctagcaatgg cgcactactc
     9361 ggcgactcca cgctgcgaac gattcagacg cagttgaaat cgatgctgag taataccgtc
     9421 agttcttcca actataaaac gctggcgcag attggtatca cgaccgatcc cagcgatggc
     9481 aaactggaac tggatgccga caaactcacc gctgcattga aaaaagatgc cagcggcgta
     9541 ggtgcattga ttgttggcga tggtaaaaaa accggcatca cgaccaccat cggcagcaac
     9601 ctgaccagtt ggctttcgac aacgggcatt attaaagccg ctaccgatgg cgttagtaag
     9661 accctgaata agttaactaa agactacaac gccgccagcg atcgcattga tgcgcaggtc
     9721 gcgcgctata aagaacaatt tacccaactg gacgttttaa tgacctcgtt aaacagcacc
     9781 agcagctact taacgcagca gttcgaaaac aacagtaatt ccaagtaatc aatattcatc
     9841 gggagacagg tcatgtacgc ggcaaaaggc acccaggcct atgcacaaat tggcgtcgaa
     9901 agcgccgtaa tgagcgccag ccaacagcag ttggtcacca tgctatttga tggagtgttg
     9961 agcgcactgg ttagagcgcg cctgtttatg caggacaaca atcagcaagg caaaggcgtc
    10021 tctttgtcaa aagcgatcaa catcattgaa aacggactgc gggtgagtct tgatgaaaag
    10081 agcaaagacg aattaaccca aaacttaatt gctctttata gctatatggt caggcgtttg
    10141 ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag aggtggaagc attaatgcgt
    10201 aatattgccg atgcctggaa agagtcttta ctctcccctt ctttgattca ggacccaatc
    10261 tgatgaacaa tgcaccgcat ttgtatttcg cctggcaaca actcgtcgaa aaaagccagc
    10321 tcatgttacg cctggcaacg gaagaacaat gggacgaact catcgccagc gaaatggcgt
    10381 atgtgaatgc ggtgcaggag atcgcacacc tgactgaaga ggttgccccg tccactacga
    10441 tgcaggagca gctccgcccg atgctgcacc tgattctcga caacgaaagc aaggtaaagc
    10501 agttattaca gattcggatg gatgaactgg cgaaactggt cggtcagtca tcggtgcaaa
    10561 aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt gctggctccg caggataacc
    10621 tcttttgaat cttaatgagc cgatggctcg cgaataatcc gattacggct acgcttctaa
    10681 tattcccctt gaatggagtc gaagaatgcg taatcccacg ctgttacaat gttttcactg
    10741 gtattacccg gaaggcggta agctctggcc tgaactggcc gagcgcgcag acggttttaa
    10801 tgatattggt atcaatatgg tctggttgcc gcccgcctat aaaggcgcat cgggcggcta
    10861 ttcggtcggc tacgactcct atgatttatt tgatctaggt gagtttgatc agaaaggcag
    10921 catccctact aaatatggtg ataaagcaca actgctggcc gccattgacg ctctgaaacg
    10981 taatgacatt gcggtgctgt tggacgtggt agtcaaccac aaaatgggcg cggatgaaaa
    11041 agaagctatt cgcgtgcagc gtgtaaatgc tgacgaccgt acgcaaattg atgaagaaat
    11101 cattgagtgc gaaggctgga cgcgttacac cttccccgcc cgtgccgggc aatactcgca
    11161 gtttatctgg gatttcaaat gttttagcgg tatcgaccat atcgaaaatc ctgatgaaga
    11221 tggcatattt aaaattgtta acgactacac cggcgaaggc tggaacgatc aggttgatga
    11281 tgaactcggt aatttcgatt atctgatggg cgagaatatc gattttcgca atcatgccgt
    11341 gacggaagag attaaatact gggcgcgctg ggtgatggaa caaacgcaat gcgacggttt
    11401 tcgtcttgat gcggtcaaac atattccggc gtggttttat aaagagtgga tcgaacacgt
    11461 acaagaagtt gcaccaaagc cgctatttat tgtggctgaa tactggtcgc atgaagtcga
    11521 taaactgcaa acgtatattg atcaggtgga aggcaaaacc atgctgttcg atgcaccgct
    11581 gcagatgaaa ttccatgaag catcgcgcat ggggcgcgac tacgacatga cgcagatttt
    11641 cacgggtaca ttagtggaag ccgatccttt ccacgccgtg acgctcgttg ccaatcacga
    11701 cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg tggtttaaac cgctggcgta
    11761 tgccttaatt ttgttgcggg aaaatggcgt tccttcggtg ttctatccgg acctctacgg
    11821 tgcgcattac gaagatgtcg gtggtgacgg gcaaacctat ccgatagata tgccaataat
    11881 cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc gcccacggtg tacagacgtt
    11941 atttttcgac catccgaact gcattgcctt tagccgcagt ggcaccgacg aatatcccgg
    12001 ctgcgtggtg gtcatgtcga acggagatga tggcgaaaaa accattcatc tgggagagaa
    12061 ttacggcaat aaaacctggc gtgattttct ggggaaccgg caagagagcg tagtgacaga
    12121 cgaaaacggc gaagcgacct tcttttgcaa cggcggcagc gtcagcgtgt gggttatcga
    12181 agaggtgatt taaatccatc cccggcggca agccggggag atttcattac ggcagtggcg
    12241 tcggtaacgg cgctttatcc agcgcggcgg cacactctgc cgtcgggcga tccacacgct
    12301 ccatcgtcat accatcgtac tcaatggtgt taccgtcacg ctcaacctcg tacagctcac
    12361 gttttacggt aacgttagtg agatcatcag acatcaatgt caacttaccc ggcacggcaa
    12421 tcacccgctg ccactgacgg caatccaggg tatcgccctc tttagtgaca atgaggctgc
    12481 ctatcgcttc cggactgacc aacgcacgtt gcggcccttt ggtctgccag taacccgtca
    12541 gccagtccgg cgcgggggtt ttcacgacat tgttatagtt ttcgacttcg gcacagcccg
    12601 ccagcaacat gagcgcacct gcaattgcga gttttttcat cattcgtcct gcatgagaag
    12661 aaaagaggat tgtggcatta aagccctgat gtcgccagcc tttccaggct cagagagaaa
    12721 tttgatctaa ccattatttt tgtgaattat taccccgaaa ataaatttgc atggtgtatg
    12781 attcgcgact cttgtttcaa accttctgag ttcagaggct acattcatgt catggcagca
    12841 attcaaacac gcctggttga ttaaattctg ggcacccatc cctgcggtca tcgcggcggg
    12901 tattctctcc acttactatt ttggcatcac tggcaccttt tgggcagtca cgggtgaatt
    12961 tacccgttgg ggcggtcagc ttttgcagct gtttggcgtc catgctgaag agtggggtta
    13021 ttttaaaatt atccatctgg aaggatcgcc attaacccgc atcgacggaa tgatgatcct
    13081 cggtatgttt ggcggctgtt ttgccgcagc gctgtgggcc aacaatgtca aactgcgcat
    13141 gccgcgcagc cgtatccgca ttatgcaggc catcattggc ggtattatcg ccggttttgg
    13201 cgcacgtctg gcaatgggct gtaatctggc ggcgttcttt accggaattc cacagttctc
    13261 gctgcacgcc tggttctttg ccatcgccac tgccattggt tcatggtttg gcgcgcgctt
    13321 taccctgctg cccatcttcc gtattcccgt gaaaatgcag aaagtttctg ccgcctcacc
    13381 gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt ctcgggatgc tggtcttttt
    13441 cggcatgctg ggctgggcgc tgctgacggc gatgaaccag cccaaactgg ggctggcaat
    13501 gctgtttggc gtcggctttg gtttgctgat tgaacgtgcg caaatctgct ttacttcggc
    13561 gttccgcgat atgtggatca ccggacgtac ccatatggcg aaagcaatca ttatcggtat
    13621 ggcggtaagt gccatcggga tcttcagtta cgtacagtta ggcgttgaac ccaaaatcat
    13681 gtgggcggga ccaaacgcgg taattggtgg tttactgttt ggttttggca tcgtgctggc
    13741 aggcggctgc gaaaccggct ggatgtaccg cgcggtagaa ggccaggtgc actactggtg
    13801 ggtcggtctg ggcaacgtga tcggctcaac gattctggcg tattactggg atgatttcgc
    13861 tccggcgctg gccaccgact gggacaaaat caacctgctg aaaacctttg gcccgatggg
    13921 cggcctgctg gtgacatatt tgctgttgtt tgcagctcta atgttgatta tcggctggga
    13981 aaaacgcttc ttccgccgtg cggcaccgca gattgctaag gagatcgcat gaaaaatatc
    14041 gttcctgatt accgtcttga tatggtgggt gaaccctgcc cttatccggc tgtcgcaacc
    14101 cttgaggcga tgccgcagtt aaaaaaaggg gagatcctgg aagtggtgag cgactgtccg
    14161 cagtcgatca ataatattcc actggatgcg cgtaatcacg gctatacggt gctggatatt
    14221 cagcaagacg ggccgaccat tcgttattta attcagaagt aatccctcat tcccgctgtg
    14281 gcccacagcg ggaacatcgt tacctctcta tcacatagat tttacccgcc ccctctccta
    14341 ttcttttcag tgtgctctca ggaggtttct atgtgtggac gctttgccca atcccaaacg
    14401 cgtgaagatt acctcgcgtt tctcgcggaa gatattgaac gcgatattcc ctacgatccc
    14461 gaacctattg gcagatacaa cgtcgcgccg ggaactaaag tccttctgct cagtgaacgt
    14521 gatgaacacc ttcatctgga tccggttttc tggggatatg ctcccggatg gtgggataaa
    14581 ccgccgctga ttaacgcccg cgtagaaact gccgcaacca gtcgcatgtt taaaccgctc
    14641 tggcaacatg gtcgggtaat ctgctttgcc gatggctggt ttgagtggaa aaaagaaggc
    14701 gacaaaaaca gccttatttt atctatcgcg ctgatggaca acctgttttt atagccgcga
    14761 taggtagcac accgtttgag cgtggtgacg aagccgaagg atttttaatt gtcactgcag
    14821 cagcagacca gggtctggtg gacattcatg accgccgccc acgagtactg tcgccagaaa
    14881 ctgcgcggga atggatgcgg caagacattg gtggtaaaga agcctcagaa atcgctacca
    14941 gaagctgtgt gccagcaaac cagttcacct ggcaccctgt gtcgcgcgcg gtgggtaatg
    15001 ttaaaaacca gggcgcggag ttaattcaac ctgtttgata agcctggaga ttatctacac
    15061 ctggatggac atcattgtac tatgccccgt acatgcccca tcacatcctc aacatcaaag
    15121 tattagagcc ttgcaaacca ttatgcgtct cagtttgtct catctatctt acgcaaaaaa
    15181 aaatagaact aatatcaagc ggcgctatat tgcgtattaa ataaaaccac ctcaattaca
    15241 ttaataaatc taaacaaata tcatgca
//
LOCUS       DAZGJT010000103        15083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZGJT010000103 DAZGJT010000000
VERSION     DAZGJT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.103"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..419)
                     /locus_tag="ACHKFK_003772"
     CDS             complement(39..419)
                     /locus_tag="ACHKFK_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054016.1"
                     /translation="MRISLASLFFLCCTVSDSVYADTDQTYDIFWGMLVKDKKDTIFV
                     RCDSPLLKMKIVRMADANTENIDKAYQIFNQKTKKSIYFAFIGNVTDAGSGKYIFNML
                     DVMETREGYCNLSDVLNAIDEQFR"
     gene            complement(492..1559)
                     /locus_tag="ACHKFK_003773"
     CDS             complement(492..1559)
                     /locus_tag="ACHKFK_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054017.1"
                     /translation="MAILHPQECWLLERIMSPEYYRRRFEGWQAFVELCERQVAEWSK
                     TIPLDVRRRPLYEQIDAVWGGRVLPNIRSTLKSVQYDFIQLQQGDLRVLQSGGNISSD
                     MKGLIDYPPDWMSPAAQKQYDRLKWRGAHYNNLIRRTSGGYWYDGELTYYYEESLHGP
                     LALPMQLPLYELDSSVYLREDDPVTVAGLYLPDIPDASAQLLYRSEHIPEAWQGRVRT
                     KYVNEAGIQEYYWESGAWAKCNWKRIRRVANRFINVPPEGFFPQGMPEELYNWPQREA
                     QYVTDRQRIAAYSGEACPHSGEWSIFVEGRQATETLEQGEQMPEWTDRKMEGEYKRGE
                     KFHVLWSLMNRHDGGSVWVEA"
     gene            complement(1601..3064)
                     /locus_tag="ACHKFK_003774"
     CDS             complement(1601..3064)
                     /locus_tag="ACHKFK_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054018.1"
                     /translation="MDDNMEMVTELQRALAFYQPPPESWYSGIQRKIEATGQWLWETL
                     QGDFNDNQSTAQVVTGTVISMIPFVDQICDVRDLIANCKKIKEGNDKNEDTTWAWVAM
                     ILTLVGLIPVAGSLIKGIFKVLFNSIRKAALSSRDIVRAIDASVSLFNKFIDLPAVQA
                     TMKWMKIYNPYTYAEKQVRELMAQLNVSVLLSKLDELMDVTGSLLEKAKSWGPESIRQ
                     PIEVIWELLVSIRSQANTMLAKALAPLNDVLEKLAARLHREGDDYYKAHTGANPHRPS
                     RLKDAEEVELLATKKPDWADIGGKEKYPQLEKASAEQKRLMDPENKEGGYPNIPDDKV
                     QTFHQMAPVEFKEGEKLYRVLDPGSSDNSFCWMREAEFKALKSKSQWRRRFAVWKSWN
                     ENGEYVVYTVPPGTTMKVWEGPAASQVREVTGKNGENIKVVLEGGSLQIVIDPTVLNL
                     DYLGKRQSTGWGYRDFSDEVDMYIGVPQLQTNIYVPK"
     gene            complement(3064..5622)
                     /locus_tag="ACHKFK_003775"
     CDS             complement(3064..5622)
                     /locus_tag="ACHKFK_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001606040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO9054019.1"
                     /translation="MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
                     YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADN
                     FLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFR
                     KGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGALPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVLDMQA
                     AIAQLENALSIAKNLSQAAEVAKAHSADLSEHESLNKALSELAKAGIVLSAPKGVGVV
                     SPRAVRLGSGESSVGLMAGTNVDVSAMKKITLTAGDSVGVFARKDGMKLYANQGKVEV
                     EAQNGGMRLTSRKDMEINSTENRVEITAKEELVLSCGGAYIRLKGGKIELGSPGNILL
                     KSVNVQKMSKARLSPAMPVLPGEGNYDLSLDLRDWEGAPISGAKYKITFESGSVLSGI
                     LDEQGYTLHTNVPPESASVEYDLPDPLPDAPWDPYASLLAAVDAEFSTNGQGAK"
     gene            complement(5634..8270)
                     /gene="tssH"
                     /locus_tag="ACHKFK_003776"
                     /gene_synonym="clpV"
     CDS             complement(5634..8270)
                     /gene="tssH"
                     /locus_tag="ACHKFK_003776"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001520117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO9054020.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLHARLAALDIEREAIEQDSVFYPEASPERLAELTDLRDELQAEAGHLEAQYQQEKAL
                     AQQIMTLRQEGTDSSELQQQLRTHQSFAPLLALDVDARAVATVVADWTGIPLSSLLKD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVAR
                     RLHQHYGLETEISNSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHH
                     KPARLRLDWDDEDGIVLEFDEK"
     gene            complement(8462..8953)
                     /locus_tag="ACHKFK_003777"
     CDS             complement(8462..8953)
                     /locus_tag="ACHKFK_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9054021.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFTFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKDPSREKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            complement(9011..10663)
                     /locus_tag="ACHKFK_003778"
     CDS             complement(9011..10663)
                     /locus_tag="ACHKFK_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO9054022.1"
                     /translation="MRNTLKQAIVLWGMVLLLVLWSVFISPSGVLRWAGAAAIVLAVA
                     ALLIYRRRQAWTEMTGDAGLSSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVPDEEQLVAQVERLLTLRPAWASQLAVAYSIMPGIHRDVAVLAGRLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERASSPWFICTGGEVQVATSTENAMPAQWIAQSGVQE
                     RSQRLSYLLKAESLMQWLDLNVLAELNGPEAKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDDALPFPDALLRQLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATANRQLLRQVGDDLHQFYAVPAEEFITKARHLSVLKDDAVMLDGYYREGEPLRLG
                     LGLYPGERIRQPVLRAIRDWRPPEQKMEVTASLQVQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            complement(10676..11341)
                     /gene="tssL"
                     /locus_tag="ACHKFK_003779"
     CDS             complement(10676..11341)
                     /gene="tssL"
                     /locus_tag="ACHKFK_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO9054023.1"
                     /translation="MKKETDINIDVLLRDTFLTVVELRQGTTVRHGIELYRHCQRQVE
                     LVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKNPLQSHFFNTLL
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
                     PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            complement(11338..12675)
                     /gene="tssK"
                     /locus_tag="ACHKFK_003780"
     CDS             complement(11338..12675)
                     /gene="tssK"
                     /locus_tag="ACHKFK_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO9054024.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALTSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALTNDSAIVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRS"
     gene            complement(12693..14231)
                     /gene="tssC"
                     /locus_tag="ACHKFK_003781"
     CDS             complement(12693..14231)
                     /gene="tssC"
                     /locus_tag="ACHKFK_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO9054025.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGEDVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVPAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFANLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWIRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            complement(14290..14790)
                     /gene="tssB"
                     /locus_tag="ACHKFK_003782"
     CDS             complement(14290..14790)
                     /gene="tssB"
                     /locus_tag="ACHKFK_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO9054026.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLNLLAVGDYSNG
                     KEQRPLSERGKVDINKNNFNSVMAEFSPAVNLTVEDTLNGNGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKI
                     APQENV"
BASE COUNT         3216 a         4402 c         3842 g         3623 t
ORIGIN      
        1 tcaaaaaaga aagcataaaa taacaatttt atccgaaact atcgaaactg ttcatctatt
       61 gcattaagca catcggacag attacaatag ccttctctcg tttccatcac gtccagcata
      121 ttgaaaatat actttccact cccggcatca gtcacattac cgataaaagc aaaataaatt
      181 gattttttcg ttttctgatt aaatatctgg tatgccttat caatgttttc tgtatttgca
      241 tccgccattc tcacaatttt cattttcaat aacggactgt cacagcgaac aaatatagta
      301 tcctttttat cctttaccag cattccccaa aaaatatcat atgtttgatc tgtatccgca
      361 taaacactgt ctgatactgt acagcaaaga aaaaacaagc ttgccagtga tatcctcatt
      421 attcatctct cctgtataaa ccagaataaa atcacccatt ccccggtttt tacgtgtaca
      481 tccagctacc gctacgcttc cacccacacc gacccgccat cgtggcggtt catcagcgac
      541 cacagcacat ggaatttctc acctctttta tactccccct ccattttcct gtccgtccat
      601 tccggcatct gctctccctg ctccagcgtc tccgtcgctt gtctaccttc tacaaaaatc
      661 gaccactccc cgctatgagg acaagcttca ccgctatagg ccgctatccg ctgccggtct
      721 gttacatact gcgcttcgcg ctgtggccag ttgtacaact cctccggcat gccctgtggg
      781 aaaaatccct ccggcggcac attgataaat cggtttgcca cccgccgtat acgtttccag
      841 ttgcattttg cccacgcccc gctttcccag taatattctt gtatcccagc ctcgttgaca
      901 tacttcgttc gtacccgccc ctgccaagcc tcgggaatat gttcactccg gtacaacagt
      961 tgggcgctgg catctgggat atccggcaga tacaggccag ccaccgttac cgggtcatcc
     1021 tcacgcaggt atacgctgct atccagctca tacaacggta attgcatcgg cagggccagc
     1081 gggccgtgca gactttcctc ataataataa gtcagttcgc cgtcatacca gtatccgccc
     1141 gaggtacgtc tgattagatt attataatgt gcacctcgcc atttcagcct gtcatactgt
     1201 ttttgcgcgg cgggtgacat ccagtccggc ggataatcaa tcagtccttt catatcactg
     1261 ctaatattgc ccccggattg cagtacccgc aggtccccct gctgtagctg aataaagtca
     1321 tactgtaccg actttaaagt gctgcggata ttgggcaaca cccgcccgcc ccagacggcg
     1381 tcaatctgct cgtacagcgg acgacggcgc acatccagcg gaatggtctt gctccactcc
     1441 gccacttgcc gttcgcacag ctcgacaaac gcctgccagc cctcaaaacg gcgccgataa
     1501 tattccggcg acattattct ttccagcaac caacactcct gtggatgtaa tatagccatt
     1561 ttatctcctg atttatcaat taattgtctg ttgatacttt ttatttaggt acataaatat
     1621 ttgtttgcaa ctgaggcaca cctatataca tatccacttc atcagaaaaa tctctatacc
     1681 cccatcctgt agactgacgt ttccccaaat agtccaaatt cagaacggtc ggatctatca
     1741 ctatctgaag tgatccgcct tccagcacga ctttaatgtt ctctccattc ttacctgtga
     1801 cttcacgcac ctgagaggca gctggacctt cccaaacctt cattgttgta ccgggtggca
     1861 cggtataaac tacatactca ccattttcat tccagctttt ccagaccgca aagcgtcgtc
     1921 gccactgaga cttactcttc agtgctttaa actctgcttc acgcatccag caaaaactat
     1981 tatcgctact gccagggtca agcacccggt agagtttctc gccttcttta aattcaacgg
     2041 gagccatctg atgaaaagtc tgcactttat catcaggaat attcgggtag ccgccctcct
     2101 tattctccgg gtccattaat cttttctgtt ccgcactggc tttttccagt tgtggatatt
     2161 tttctttacc cccaatatcc gcccagtctg gctttttggt agccagcaat tccacctctt
     2221 cagcatcttt caggcgagaa ggccgatgcg gattagcacc agtatgcgct ttataataat
     2281 cgtccccttc ccggtgcaga cgggcagcca gcttttccag cacatcattt aacggtgcca
     2341 gcgcttttgc aagcatggtg ttggcttggc tgcggataga caccagcagt tcccatatca
     2401 cctctatcgg ctggcgaatg gactccggcc cccaagattt agccttttcg agtagcgaac
     2461 cagtgacgtc cattaactca tccagcttgc ttaacagcac cgatacgtta agttgcgcca
     2521 tcagttccct gacctgcttc tcagcatacg tatacgggtt ataaatcttc atccacttca
     2581 ttgtcgcctg cacggctggc aaatcgataa atttattaaa cagggaaacg gaagcatcaa
     2641 tcgctcgtac aatatcccta ctggaaagcg ccgcctttct tatcgaatta aacagcactt
     2701 tgaagatccc tttaattaaa gaacctgcca ctggaataag accgaccagt gtcaggatca
     2761 tcgctaccca tgcccaggtg gtgtcctcgt ttttatcgtt gccttccttt atcttcttac
     2821 aattagctat cagatcgcga acatcgcaga tctgatcgac aaagggaatc atggaaatga
     2881 ctgttcccgt cacaacctga gcggtactct gattgtcgtt aaaatctcct tgcaaggttt
     2941 cccagagcca ctgcccggtc gcctcaattt ttcgctgaat acctgaatac caactttccg
     3001 gtggcggttg gtaaaaagcc agtgctcgtt gcagttccgt caccatctcc atattgtcgt
     3061 ccattacttt gccccttgtc cgttcgttga aaattcagca tccacagcgg caagcaacga
     3121 cgcataagga tcccaggggg catccggtaa cgggtcagga agatcgtatt ccacagatgc
     3181 ggactcagga ggaacattgg tatgcagtgt atatccctgc tcatcaagta ttcccgatag
     3241 aacggaacct gattcaaagg tgattttgta cttcgcaccg ctgatgggcg caccttccca
     3301 gtcccgaaga tccagcgaca aatcgtaatt gccttccccc ggtaaaacag gcatcgctgg
     3361 tgacagtctg gctttactca tcttctgcac attcactgac ttcagcagga tattcccagg
     3421 actccccagc tctatcttcc ccccctttag ccggatatac gcaccgccgc acgacaacac
     3481 cagctcttct tttgccgtta tctccacccg gttttccgta ctgtttatct ccatatcttt
     3541 acgcgatgtc aggcgcatcc ctccattctg tgcttccact tccacttttc cctgattcgc
     3601 atacagcttc atcccgtcct tacgggcaaa cactcccact gaatctcccg ccgtcagtgt
     3661 tatcttcttc atcgccgaca cgtccacatt tgttcccgcc atcagtccca cactgctttc
     3721 cccggaaccc aaacgcaccg ctctaggact cactaccccc acgcccttcg gggcagacaa
     3781 cacgattccc gcctttgcca gttctgataa tgctttattc agagactcat gctccgacag
     3841 atcagcgctg tgcgcctttg ccacttccgc cgcctgtgac agattttttg ctatcgacag
     3901 cgcattctcc agttgcgcta tcgctgcctg catatccagc acctgaccct gcgccttatc
     3961 ctgcgcatcg gcgctgataa atatcccttt cttagcccgc accgcgcccc acagatcggt
     4021 acgcagttca aagccttcac cgcgctgctg tttcccggca tccaccagat ggccgaggtt
     4081 tagctggctc ttgccgccgt actcggtact gaccttgata tgctctttgc cgcgctcatc
     4141 gtcgaggcgg attttgttgt tcgccggggt gcgtagcacg ttgcgcttgt agttctgaat
     4201 ggtcacatgg tcagtgtgtg ccgagtcgtg ttttacgcct gcaatataag gtcggtccgg
     4261 gttgccttcc tcaaaggcga tactgacttc cgttccagcc agcagcggca ggtgcaggcc
     4321 gtaggtgtct cccgcatacg ggcgcgactg gcgcacccac aggctctcat agcccggttt
     4381 ccacgtatcg cggtcaaagt cgaggttgac ccggtagcgc ccgtccttgt cgatatgggc
     4441 gtagatgtcg ttcttcaccg tactggtgac ccgcgccggg agcgctccgg ccattacggg
     4501 gcgggggata agcgccggac ggtagccgta gcgctctgag tacggtatcg ccgtgaacgt
     4561 cagctcatag ctgcggtcac gcgccgcgct ggtcgtcacg ccggtaatca gcacgccctt
     4621 gcggaacacc gcaggcgcgt cgtcgccctg aacttttatc tccagccccg gcatcagcag
     4681 tgaactggtg ctctggcctt tgagaatggc ctgttcattc aggtagcgtt cgtgacggat
     4741 acgggcataa aacgcgccgc tttccgctgc ctctttatcg ccttgttgca ggaagttgtc
     4801 ggcatagtgg taggcttcgc cgtaagtggt gttatcgccc ccggtggcgt catgctggcc
     4861 tgtggtcatt tccgccgtgg cttcacggta gttgtagtcc cgcgtggaga cacttttttc
     4921 caccacgctg tacgcggtgt tcagccccca tacggcttcc gtttcaccat caaacagccc
     4981 agaaggatgg cgaagcggca gcgtcagccc gcgctcgtag ccgctctggt cgtcgtaaaa
     5041 ttcgataact tcgattttca accgggcgtc ggtggcaaag cggaaccaga tacccacctc
     5101 tgacagcagg cgtgaaacaa acgtcaggtc gtcctcaccg tactgcatca cctgttcgcg
     5161 ggccgggtat tcgcttttga ggttaaacac gaagtcctga ccgcgcatct ggtgccgctc
     5221 gcgcaggatt ttttcgacga tttgcggcac cgtctggttc tggtagatgg cgttctggcg
     5281 gctgcgggta agcagcgcca tacggggttc aaggcgcact tcgtaacggg cttcgtcctg
     5341 tgacgatgag agatgtttaa agccggtaat gacgccgtgc agggtgcgca gggcagtctg
     5401 taccggcatt ccctgtaccg gcggtgccgt caggctgaat gcgccatcct gcatcagcac
     5461 cgactccggc gcgatggctt tatcgctgct ggtaaactga atgtcgtagc ggaacggggt
     5521 actcagttgc tcgcggcctt caaaggtcag cacatccacc ggactttgca ggccgcgaat
     5581 tttgagcttg tggtgtgcgt ggtcgaagaa ctgtacgttc ataagagcct ccattatttc
     5641 tcatcaaatt ccagcacaat cccgtcttca tcatcccagt caagccgcag tcgggcaggc
     5701 ttatgatgca cggcctgctg cgccagcaac tgctggctta agaccggcag gatttgctgg
     5761 ttcagcaggc tgtcgatatt acgcgcaccg gtgtccggca gcaggcaggc ggcggtcagg
     5821 gcgtcgtaca ggctgttact gatttccgtt tccagcccat agtgctggtg aagacggcgg
     5881 gccacctgcg ccagtttcat ttccacaatg gcgcgcatcg cctcctgtcc cagcgggcgg
     5941 taaatcaccg tctgaaaacg cgccatcagt gctggctgga agtgctcgcg cagcagggga
     6001 tacagcagtt catgcaggtc tgcgtccgtg gcgtccggtt tttcctccag cagttgcatg
     6061 atgtggtcgc tgcccagatt agccgtcatc agaatcacgg tgttgcggaa gtcaatttcc
     6121 cggccttcgc cgtcgcgcat aaagccccgg tcaaatacct gatagaacag gttcattacg
     6181 tcgcggtgcg ccttttccac ttcgtcgagc agcaccacgc tgtaagggcg tttgcgtacc
     6241 gcttcggtca gcacgccgcc ctgaccgtag ccgacatagc ccggaggcga acctttcaac
     6301 tgggaaacgg tatgcggttc ctgatattcc gaaagattaa tggtgataag ggatttttca
     6361 ccgccaaaca gagtgtcggc cagtgtgagc gcagtttcgg ttttaccggt gccgctgggg
     6421 ccggtcagca ggaatacccc ctgcgggccg ttctccggcg tgaggccggt cttagccgcc
     6481 cgcagccgct gtgcgatggc gcagagcgcc gggctttgcc cgacaacgcg gttttcaaga
     6541 ctctgttcca tactgagcag gtcgctctgc tcgtccttga gcagggatga gagtgggatg
     6601 ccggtccagt ccgccaccac cgtggcgacg gcgcgggcgt ccacatccag cgccagcagc
     6661 ggtgcaaagc tctgatgcgt ccgcagttgc tgttgcagtt cagagctgtc tgttccttcc
     6721 tgacgcaacg tcataatctg ctgcgccagt gccttttcct gctgatactg cgcttccaga
     6781 tgcccggctt ctgcctgtag ctcatcacgc aaatcggtca gttccgccag ccgctccggg
     6841 ctggcttcgg gataaaatac gctgtcctgc tcaatcgctt cccgctcaat atccagtgcc
     6901 gccagtcgcg catgaagctg cgtcaacggt tccggcaggg tgtcaaggct catgcggact
     6961 cttgccccgg cggtatccag caaatcaacc gccttgtccg gcagctggcg tccggtcaga
     7021 tagcggcgcg acaggcgcac ggcggtctga atggcgctgt ccagcatatg cacgccgtgg
     7081 tgctgtgcat agcgagcctt cagtccccgc agcatcagac aggcggtgtc atcatccggc
     7141 tcgtcaacct taaccatctg gaagcggcgc tccagcgcgg cgtcgcgctc aaaatactgt
     7201 ttgtattcgc tccacgtggt cgccgcgatg gtgcgcagtt cgccccttgc cagtgccggt
     7261 ttcagcaggt tcgccgcatc cgcgccgcct gcctgattac ccgcaccgat aatggtatgg
     7321 gcttcatcaa taaacagcag taccggctcc ggtgatttct gcactgcatc gatgacattt
     7381 ttcagccgct gttcaaattc acctttaacg cccgcgccag cctgtaacag accgaggtcc
     7441 agtgtgcgga tatgcaccgt tttcaggctg tccggcacgt tgccttcggt gatacgcagc
     7501 gccagccctt ccaccagcgc ggttttgccc acccccggtt ctcccaccag aatcgggttg
     7561 tttttgcggc gacgggagag gatatcgacc atctggcgaa tttccgtgtc gcgcccgaat
     7621 accgggtcga ttcgcccgct gcgggctttt tccgtcacgt cttcggtaaa gcggttaagc
     7681 accgccagca gggcgtcatc ctgtttctcc gtcagcgttt ttgccggtgc gtccgccgtt
     7741 gccgtctccg gcagtgcaga ggtcagcggt tcggtgacct gtggctgacg tgccggacat
     7801 tcatccgact gcgcatccag caggggacgc agccgctcaa gctgcacgcg gctcagactc
     7861 agcaataccc acaggtcatt acaggcgggc agcatcggtt tttctgtcag cgccatcagc
     7921 agatgctggc tgcggatttg tggatcgtcg ccttccagtg acgctatcag ccacgcctgt
     7981 cggataagcg ctgccagcgg ttcagaaagt tgaggacgtt cgcggaccgg gcggggtaag
     8041 gtgtccagat gtgccagcag agactgccag agcgtgtcga tatcccattc atagcggcgg
     8101 gcaatcaccg tgatatcgcc ttctccctgc tccagcagtt tcagcagcca gtgttcaatg
     8161 gttatttccg catgggcgcg ggtctgacac agcgaggcgg cggcgtccag cgcccgtgca
     8221 cagtatgggt taaggcgacg cagcagcgcg gcggggtgat ttcctgtcat agaagggttt
     8281 ccttatcgtg ttctgtattc ctgcgatccg cgttcaggca cgctaccgcc catagccgtt
     8341 acctcactca ggcccatcag gtatcaccag attgtgggta tgctttgctc agtaccggtg
     8401 cctgcggcac tggacaaagc acacaacaaa aaggcagaca gtttcttgtc tgccgcagtt
     8461 atcaggcgga aggacgctca ttccacgagt cggaatgaat gatgttgccg tctttgtaag
     8521 tccaggtgat tttctcgtag cggaactcaa tacgttccag gtggttatgc ttctctctgg
     8581 aaggatcctt gatgtcgtgc ataagcggag cgactctgac cagcttgacg ttatcaagcg
     8641 tgatgttgaa gtactccact tcctgaccgg catcgttgat gcggtaaaac ttaaattctg
     8701 ccgttttcag ggtctgtccg gtggtcacag ctttgtagag atacgggctg gaggcatcga
     8761 tttctttggt aaacgtaaag ggcttgtgag tacgggtacc ggtcagtttg ccggtgttat
     8821 tgtcggtcgg gatgtacaca tcgtgatcca gcgccaccac ttcgatgcta ccttcgcgcc
     8881 cctgaacgtc cacggaacct ttgatatccg cgccgccgtc atctttcagc cagagataag
     8941 caggaatagc catgagaatt actctccttg ttagtgtgat tgaagcaccg ccttcttccg
     9001 gcggtatgtt ttatttcacg tcctgacagg cgtcagaacg cggaacaaga ctgatttcga
     9061 cacgccggtt gactgcccgg ccctgtggcg tgtcgttggt cgccgcaggc tggctctcgc
     9121 ccagcccctg tacggcaaaa caggtcgccg ggatgtcgct ggtctgcagc atccagttgc
     9181 gcaccgcttc ggcacgccgc agcgataact gctgattgct tttttcatcg ccggtggcgt
     9241 cggtatatcc ggccacgagg atcagccagc ccggttttgc ccggatgttc accagcgcat
     9301 ccaccagcac ttttgtcgag ccgtctttca ggcgggcctg tccgacgtca aacagcgaca
     9361 tactgtcaag acgcacggtc tgaacctgaa gcgaagccgt cacttccatt ttttgctcag
     9421 gcggacgcca gtcgcgaatg gcgcgtaata ccggctggcg gatgcgttca ccggggtata
     9481 accccagacc gagacgcagc ggttctcctt cccggtaata cccatcgagc atgaccgcat
     9541 cgtctttcag caccgacagg tgacgggctt tggtgataaa ttcttccgcc gggacggcat
     9601 aaaactggtg cagatcgtca ccgacctgcc gtaataactg gcgattcgcc gtggctgaca
     9661 ggcacagcgc ggcgataccc gccacggtag taatacccag catggtcacg caggcccgtc
     9721 gcagcggggt aaagcccgac tgacgcggca actgccgtaa cagggcatcc gggaacggca
     9781 gcgcatcgtc tgtgccggtg tccgcgatat ccgtcgtcag gccggttctg gcggtgatcc
     9841 acaactgcca caggttgtta tccaccgcag gcaacgaggg gaccagcccc acggccatcg
     9901 ccagtggtgg acatttcgcc tccgggccgt tcagttccgc cagcacattc agatccagcc
     9961 attgcatcag gctttcggct ttcagcagat aagagagtcg ctgactgcgt tcctgtacgc
    10021 cggattgtgc aatccactgc gcgggcatcg cgttctctgt ggatgttgct acctgaactt
    10081 cgccgccggt acagataaac cacggtgaac tcgctctttc cggcaacggc gagccggaca
    10141 gcccgctcca gagaagccag gggacgttta cgcccgcccg gcgacgcacc gtcgccatac
    10201 tgtgggcaaa ccgtcgcagc cgtccggcca gaaccgccac atcccggtgt atgccgggca
    10261 tgatgctata cgccacggca agctgcgatg cccacgccgg gcgaagggtc agcaatcgct
    10321 ccacctgcgc cacaagctgt tcttcatcag gaacatgcag atacagccct tctgaaacct
    10381 ggcggaccgg gctgtcagtg aacaggtgcg ccgacatatc accacagacc agcactaccg
    10441 gctgtcggta ggtttccggc ggcagcgatg acaacccggc atcgccggtc atctccgtcc
    10501 acgcctgcct gcgccggtaa atcaacaacg cagcaaccgc cagaacgata gccgccgcac
    10561 cggcccatct cagcacgcca gacggactga taaacaccga ccacagcacc agcagtaaca
    10621 ccattcccca cagcacgatg gcctgtttca gcgtgttcct cactcacgac tccctttaac
    10681 gcaggctttg cggcagcagt tcatccacca gtacattcag ccagtggtgt agcccccacc
    10741 agacgcccgc cagtagcagc gccagtgtta ccagccagaa aaacggcgaa tgcagcaaag
    10801 ggtgctgacg ggtggataac ggcacgttca gtaccgccgt ttccggcagc acaccaaatg
    10861 gcgcgacctg cccgttgagc gtcgaaataa gctggtcgcg ctccggggct gccggatcct
    10921 gataacgccc gcgaaatccc agcagcagaa cgcggtgaaa gcaggtcagc acggcctgcg
    10981 cgggggcggg ctcctgtagc acctgcttca tccgctcgta cagcagctca ccggccagca
    11041 gggtgttgaa gaaatgcgac tggagtgggt ttttcatcca gatggcctga ccgtcgtcca
    11101 tgccactacg gctgagtacc gtttcgtcca gcagggcgca ctgcgcgtaa gttatgtgct
    11161 ccacgttctc ccgactgaat ccggcatctt tcagacgttc gcggaccagt tccacctgcc
    11221 gctgacagtg gcgatacagc tcgataccgt gacgcacggt ggttccctga cgcagttcca
    11281 ccaccgtcag aaacgtatcg cgcagcagca cgtcgatatt gatatccgtc tcttttttca
    11341 tgagcgcagt accgcaaaca gttcaggttt caggtcgccc attgttcccg gcgcataaat
    11401 catgcagcac ccggactcca gcatcgattt agccgcgtca ctgtgcatat ccagcgcaaa
    11461 atactggttc tccagccgca gcggcagcgc cgccggaacc gaggtgagtg gcaccagtgg
    11521 aacaccgttc agcgccacgt tgataagcag cgtgacgtca tcaggcgcac cgattttgca
    11581 gaccagcggt agctggtgca gtacctgatg cgcaggcagt gaggaacgaa cggacagata
    11641 aaaatcggcc tcttcccgca ggcgcggatc gtgcaggtcg gctttccagc ggttgcccgg
    11701 cagggacgcc agatccagcg cgatcacccg cgacggcagg ctggcttcca gcagctcgct
    11761 gagcagggag aacaggggcg gaaagacggt ggacggcgat tcgtggacat agggcggaac
    11821 ggcgctgacg ttatgttcaa gggaaaacgt cagcagcgca ccggcaagcc tcgccagctc
    11881 ccgccagacc agttcgggat gaaccgccgg gtagcgcagg aaatcactca gtaccggctc
    11941 ggcggagttc agcgcgttaa gcagccagaa cagggagaca tcggcgacgg cgaagtccgc
    12001 catacgctgg ttgctttccc ggcgcatcgc catcaggcgc tgacatttgg cccgtagctg
    12061 caacagcagc gtgtccaggc gctgtaccag cccgtcatgg gcgttaaacg ccagtaacgg
    12121 cggaatatag ctctcatcct gtgtccagtt gccctgaaca tcccgcacca gacgcgccag
    12181 tgggcaggtg atgtaatcct gattgttatc atgagcaaag cgcagggaca gcgcatgacg
    12241 ctcaacggcc atatcttcac tgtcactgcc gtagatatcc tgcacctttt gccattcctg
    12301 acgatagcgc agcggtcttt cagtctgctc accctgaccg agattaccgc cgttaccatg
    12361 cgcaagcggc agcgccagca gaacaatcgc gctgtcgttc gtcagcgcgc gcagatcgca
    12421 ggcgagcggc agaacatcgc tcacgtcaga atcgatcagc gtgccatcag caaaacgcag
    12481 gcgtacagct tgcgttttca gctttccgga cgtcagggca tcgcggtcaa agagcacatt
    12541 cgcgatcccc cacggatgac gaatacaaag ttgcgcaatg ctgtcattgc tgaacgcttc
    12601 ccagcgggcc tgctgctgaa actgctgaga ggacaggaat gtcccctcag cccacagtgg
    12661 tcgatgaatt ttcatatcac accactgccc gatcaggctt tcgctttcgg catctgggaa
    12721 accagtgaca ggttgatatc catcccttca atctggaaat gcggcacggc aaagagtctg
    12781 acgcggaaga agcccggatt gtcctcaata tcttccacga taaccttgcc gtcgcgcagc
    12841 gggtgagacg cctgcagttc atcacccgga tcggtcatct ccgtcaccag tgtgcggatc
    12901 caggtgttca gttccagttc cagtacgcgg cggtctttgg tggtaccgat attctcgcgc
    12961 tggatgattt tcaggtaatg cgcaatgcgg gacagcagga aaatataggg caaacgggca
    13021 ttaatacggc tgttggcggt ggcgtcagcg gtatcataca acgccggctt ctgggcggag
    13081 tttgccgaga agaagcaggc gtaatcgcga ttcttataat aagagagcgg aataaagcca
    13141 aggttggcaa attcaaattc gcgggtttcc gggatcatca cttcggacgg aattttgacc
    13201 tgattgccgg tgccgagatc gtaaagatgg atgggcagat cggccactgc gccacctgcc
    13261 tgtggaccac ggatctgcac acaccagcca ttattcacaa agctcttcac catattggcg
    13321 gcaaaggcga acgaggcgtt tgtccacagg tacttttcgt gatccgggcc tttgacttct
    13381 tccacatagt taaagctgcg taccggcacc gtgtccggcc catagggcag acggcccagc
    13441 acgcgcggca tcactaatcc cacatagcgg ctgtcatccg tatcgcggaa cgacttccat
    13501 ttaatatatt ctgcgcggtc aaagtagttg ccgatatctt taatcgcggc gacttcttcc
    13561 atcgaatttt taaggaagaa tttcggtccg acagaaccga taaaaggcat atgggaagcg
    13621 gcaggcactc tggaaatatt acgcagcagg gcaatatcct gcgggctgtt atcaaattca
    13681 tagctggata ttaatgaggc tacaggttcg ccacccggcg tatcatattc ctgaatgtat
    13741 gtatggcgat ataaaccact ctgaatgatt tccggtgaat catcaaaatc ctgacgcaga
    13801 tgctctttgc tgatatccag caattcaatt ctgacatttt tgcgaaaatc agtgcgctgt
    13861 acgacgaacc atgtaccacg ccacagcgac tcgactttct ggaattcagg gtggtgcatg
    13921 accgcatcaa gctgtttact gattttctga tccagatcgt cgatatgaaa atccagcagg
    13981 ctcttgtcga gcttgaccac gtcctcgcct gatttcgcca gacaggaaag cagaacgtga
    14041 atcgctgctg tcacgcgctc atcgggtgag gcatcagcca gcgtttcgct gttttgccat
    14101 aaatctattt cctgaatgga agagaccggg gttaaattaa ttttatcgaa cagggactga
    14161 taaacgccgc cggactcagt ggtggtgagt gttgcagttt cagaggtggc atgttcttgt
    14221 tgtacagaca tcagcatttc cttttcagtg atcctttctt ctccggttta acctgaatac
    14281 gcgttcgggt taaacgttct cctgcggcgc aattttcgcc agttcatcac gcagttctga
    14341 actgagcgtc gggtctttca ggatattttc cagttcacgg cgaaacgttg cgttatccag
    14401 cagattggat ttcaggtcac gtagtaaatt gcgcatagcg agtaatacac gaagttgcgg
    14461 aatatttttt gctacctgtt cgggttcaaa atccttcagg cttttaaatt caagcgcgat
    14521 attttgttca ttaccgtttc cgtttagcgt atcttctact gttaaattaa ccgcaggcga
    14581 aaactcagcc atgacgctgt tgaagttgtt tttattgata tcaaccttgc cccgttcgga
    14641 cagcggacgt tgctcttttc cgttactgta atcgcctacg gcaagaaggt tgagggggag
    14701 ttcaactttc tttttagcat tgcctgtatg cagatcaagc ttgatattta cacgagcggc
    14761 gggaacttca ttctggaaac tgtcggccat atccttttcc tcatcatcca ctttttctga
    14821 tggttattga cataattata ttcaggggat tatcggatga tttaaataaa aagcaatgat
    14881 gaggaaaata tacaaatgaa taatgtattt ttcaggggaa tagatacagt tgtatcattt
    14941 tagatattgt ttggcctctt gattattttt cggaagaggg cggcattatc tggtgagaga
    15001 atttggcatg aaacttaaaa taataacagg ataagcttca caaatttgca cttttaagaa
    15061 attgggtagt atgagagtct att
//
LOCUS       DAZGJT010000104        15066 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZGJT010000104 DAZGJT010000000
VERSION     DAZGJT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.104"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..110)
                     /gene="shoB"
                     /locus_tag="ACHKFK_003783"
     CDS             complement(30..110)
                     /gene="shoB"
                     /locus_tag="ACHKFK_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO9054027.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLLLLFL"
     gene            complement(531..911)
                     /gene="acpS"
                     /locus_tag="ACHKFK_003784"
     CDS             complement(531..911)
                     /gene="acpS"
                     /locus_tag="ACHKFK_003784"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO9054028.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWEIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(911..1642)
                     /gene="pdxJ"
                     /locus_tag="ACHKFK_003785"
     CDS             complement(911..1642)
                     /gene="pdxJ"
                     /locus_tag="ACHKFK_003785"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO9054029.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLAEAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFATSLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(1654..2382)
                     /gene="recO"
                     /locus_tag="ACHKFK_003786"
     CDS             complement(1654..2382)
                     /gene="recO"
                     /locus_tag="ACHKFK_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO9054030.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLLFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(2394..3299)
                     /gene="era"
                     /locus_tag="ACHKFK_003787"
     CDS             complement(2394..3299)
                     /gene="era"
                     /locus_tag="ACHKFK_003787"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO9054031.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(3296..3976)
                     /gene="rnc"
                     /locus_tag="ACHKFK_003788"
     CDS             complement(3296..3976)
                     /gene="rnc"
                     /locus_tag="ACHKFK_003788"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO9054032.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(4248..5222)
                     /gene="lepB"
                     /locus_tag="ACHKFK_003789"
     CDS             complement(4248..5222)
                     /gene="lepB"
                     /locus_tag="ACHKFK_003789"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO9054033.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(5238..7037)
                     /gene="lepA"
                     /locus_tag="ACHKFK_003790"
     CDS             complement(5238..7037)
                     /gene="lepA"
                     /locus_tag="ACHKFK_003790"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO9054034.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRGNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(7235..7714)
                     /gene="rseC"
                     /locus_tag="ACHKFK_003791"
     CDS             complement(7235..7714)
                     /gene="rseC"
                     /locus_tag="ACHKFK_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO9054035.1"
                     /translation="MIKEWATVVSWENGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVSCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(7711..8667)
                     /gene="rseB"
                     /locus_tag="ACHKFK_003792"
     CDS             complement(7711..8667)
                     /gene="rseB"
                     /locus_tag="ACHKFK_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO9054036.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASEALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDIGSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGTAQ"
     gene            complement(8667..9317)
                     /gene="rseA"
                     /locus_tag="ACHKFK_003793"
     CDS             complement(8667..9317)
                     /gene="rseA"
                     /locus_tag="ACHKFK_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO9054037.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(9350..9925)
                     /gene="rpoE"
                     /locus_tag="ACHKFK_003794"
     CDS             complement(9350..9925)
                     /gene="rpoE"
                     /locus_tag="ACHKFK_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO9054038.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(9922..10077)
                     /gene="rseD"
                     /locus_tag="ACHKFK_003795"
     CDS             complement(9922..10077)
                     /gene="rseD"
                     /locus_tag="ACHKFK_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO9054039.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            10333..11955
                     /gene="nadB"
                     /locus_tag="ACHKFK_003796"
     CDS             10333..11955
                     /gene="nadB"
                     /locus_tag="ACHKFK_003796"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO9054040.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHIQPNGAESYHLTREGGHSHRRILHAADATGREVETTLVSKALNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTKGPVPIVPAAHYTCGGVMVDDYGRTDVDGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRCMPYAHDVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(11940..12677)
                     /gene="trmN"
                     /locus_tag="ACHKFK_003797"
     CDS             complement(11940..12677)
                     /gene="trmN"
                     /locus_tag="ACHKFK_003797"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO9054041.1"
                     /translation="MSQSTSVFRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVMIDAVELESEAAAQAQENIYQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            12809..14143
                     /gene="srmB"
                     /locus_tag="ACHKFK_003798"
     CDS             12809..14143
                     /gene="srmB"
                     /locus_tag="ACHKFK_003798"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO9054042.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLKDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(14176..15057)
                     /gene="yfiE"
                     /locus_tag="ACHKFK_003799"
     CDS             complement(14176..15057)
                     /gene="yfiE"
                     /locus_tag="ACHKFK_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9054043.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQLLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFTVANELASGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHIFIQCVEECFLPG"
BASE COUNT         3661 a         4043 c         3710 g         3652 t
ORIGIN      
        1 cggacgcacc ttaagtacgt ttccttgtgt tataagaaca aaaggagcag ctgtaaaaca
       61 gcaatgatga ttttgatgac ccgtttaatc aggtatcggc aatcagtcat tcgtttttcc
      121 ttaaacaagg aatgcagccg ttcagattag cccttacatc tccccaaaac tgaacgtgcg
      181 agttattgga atcgagtgct gcactccaca ccagagcttt gacgacacca ctcgtttcaa
      241 tgggggaatt cagtggcatg gtgtaaagca cagcaaaatt gccaataacg aaaccgattt
      301 taatgtactt atcaaaaagc ggtcacgtta atcttttcat caatgtcgat ttcttcgtaa
      361 aggctcgcgt tttttatgtc agagattgca agttgcttgt acaggtcaac gaaattgatc
      421 cataatcatt ttcgttggaa tcgtgtgctg cacaaaatta aagttaaaaa gtaaaacccc
      481 cgttccttac cagttcgggg gttttacttt ataaagagaa cggtattatt ttaactttca
      541 ataattaccg tggcacaagc atagtggcgc tcatcggcaa gcgttacatg catatttaca
      601 acgccgagct tttccgccag ttttaatgcc tcaccccata gccgtagccg tggtttgccg
      661 agctcatcat tgaatacttc aaactgatta aacgccagac cattgcggat ccctgtgcca
      721 aatgcttttg ccgccgcttc tttcacagca aaacgcttcg ccagaaaacg cactggctgg
      781 tggtgcgttt tccagatttc ccattcgtta tcgcttaata cgcggcgtgc caggcgctca
      841 ccggatcggg cgatcaccgc ttctatgcga gcgatctcta caatatccgt acctaaacct
      901 aatattgcca ttagccacgc gcttccagca tcagacgttt catttctgcc accgcatctt
      961 tcagtccggt catcactgca cgaccaataa tggcatgacc gatattcagt tcatgcatct
     1021 cagggatggc ggcaatggct ttcacgttgt gataggtcag accgtgtccg gcgttaactt
     1081 tcagaccgag actagtggca aaggtcgcgg ctttggcgat acgcgccaac tcttgcgcct
     1141 gttcggcgtc agttttggca tcagcatagc aacctgtgtg gatctcgata aacggtgcgc
     1201 caacctctgc cgcagctttg atctgctctt catcggcgtc gataaacaga gaaacctgga
     1261 tcccggcctc cgccagacgt ttacaggcat cgcgcatttt gtcacgctgc cctgcgacgt
     1321 ccaggccgcc ttcggttgtt acttcctgac gcttttccgg taccagacag caaaaatgtg
     1381 gcttcgtctc aacggcgatc gccagcatct cttcggtcac cgccatctcc agattcatgc
     1441 gggtatccag cgtctgacgc aggatgcgca catcgcggtc ggtaatgtgg cggcgatctt
     1501 cacgtaaatg cacggtaatg ccgtctgctc ccgcctgctc ggcaataaac gccgcctgca
     1561 ccggatccgg gtaagcagta ccgcgtgcgt tacgcagcgt tgcaatatgg tcaatgttga
     1621 cgcctaacag taattcagcc atgacaatcc tcatcattca taatgtgttt tcaccgttcg
     1681 cttaggcata aactgccgga acagttccct gctctttaat ggtttaccgc caagatacgg
     1741 cttaagcgcc atgcgggtaa agcgtttcgc ggcgcgcagt gtgtctgcgt caggaaattc
     1801 ccgcgcgttt aacgctttta actgccttcc ggtgaacgtt ttattgtcga taacgacgct
     1861 tgcgataaac cctttttctt cgcgataacg atacgtcatg gtgtcatcta ccggctcgcc
     1921 gctacccgca caatgggtaa aattgacgcc atatcccaga tgcccgagca gtgccagttc
     1981 aaagcggcgc agcgcgggtt ctggcgtacc agtggcacct gcaagtgact gaatgcagtg
     2041 caagtaatcg aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc gggagagaag
     2101 ttcgttgatg tacagaccgc tgtaaagcgt gataccgctt aatggcagcg ccagcgagac
     2161 agcttcagca ctgcgcagtg ttttgacttc accacgccca ccgaagagaa gcaagagagg
     2221 ggtgaaaggt tgtaatgcac ctttcagagt agagcgttta gagcgtgcgc ctttggcaac
     2281 cagacgcacg cgacccgatt cctccgtgaa gacgtccagc atcaggctgg tttcgctcca
     2341 cgggcgacta tgcaggacaa atgcgcgctg ccagccttcc atcggagtta ctcttaaaga
     2401 tcgtcaacat aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc agatttcact
     2461 tttacccaca gctcaaggtg aacaggcgct tcaaacattt cctgcatgtc tttacgcgct
     2521 tcaatcccga tggttttgat cttggcccct ttgttgccaa taaccatctt cttctgccct
     2581 tcacgctcaa cgagaatcaa accgttgatg tcataaccgc cgcgttcgtt agagacgaaa
     2641 cgttcgatct ccacggtcac ggagtacggc agttcagcgc cgaggaaacg catcagtttt
     2701 tcgcggatga tttcagacgc cataaaacgc tgtgagcgat cggtgatgta atcttccggg
     2761 aagtgatggg tcgcttcagg cagatgctta cgcacgattg ctgcgatagt gtcgacattc
     2821 agcccggttt cggcagagat cggcacgata tcgaggaagt tcatctggct tgccaggaac
     2881 tgtaagtgtg gcagcagatc ggctttttcc tgcacgttat ccactttgtt caccgcgagg
     2941 attaccggcg ctttgccatc gcgcagtttg ttgagcacca tttcatcgtc cggcgtccag
     3001 cgggtgcctt ctacgacaaa aatcaccagc tcaacgtcgc cgatagagct gctcgccgct
     3061 ttgttcatca ggcggttaat ggcgcgtttt tcttccatat gcaggccagg ggtatcaacg
     3121 tagatcgcct gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg agttgtttgc
     3181 gccttgcggg aggtgatgga gatcttctgc cccagcagtt tgttcaacaa tgtggatttg
     3241 ccaacgttcg gacgtccgac gatagcaata aatccgcagt aacttttatc gatgctcatt
     3301 ccagctccag ttttttcaac gcctgttcgg cggcagcctg ctcagcctta cgacggcttg
     3361 aacctgtgcc aaccaccggt tcactcaggc cgctgacctg gcagtggata gtaaattcct
     3421 gatcgtgtgc ttcgccacgt acctggacga ccagataagt cggcagcggc agatggcgac
     3481 cctgcaaata ttcctgcaga cgcgttttcg gatctttttg tttatcgcct gggctaattt
     3541 cgtccagacg agtttgatac cagttgagga ttaatttctc gacggtttga atatcactgt
     3601 cgaggaatac gccaccaatt aatgcctcga cggtgtcagc gagaattgac tcacgacgaa
     3661 atccaccgct tttaagttca cctggcccta aacgtaagca ctcacctaac tcaaattcgc
     3721 gcgccagttc agccagcgta ttaccacgga ccagcgtggc gcgcatccgg ctcatatcgc
     3781 cttcatcgac acgagggaaa cggtgataaa gcgcattggc gataacgtag ctcagaatag
     3841 agtcacctaa aaattctaaa cgctcgttat gtttactgct ggcactacga tgagttaatg
     3901 cctgctgcaa cagttcctga tgattaaaag tgtagcccag cttccgttga agccgattaa
     3961 ttacgatggg gttcatgcga taccaataaa taaatgcgtc aacaattcag cacacgaaac
     4021 agacctgata tacatggctc ggctaactgc ttcactgcag tttcagttta ccggtatatg
     4081 gggaccaacg ctgtttcgtg tgccgtggca acctggaggt gccaacctta aacttcgggg
     4141 gaatattcta tacacaacga cgggggatgt cgttagccac gggagattta tctcataaat
     4201 aattcacgtt gtcgccataa cggcgacaac gtgaacgaag atggctatta atggataccg
     4261 ccaatgcgac ttaagcgcac accagtcggc cattcgcctt cttgcttatc gaagctcatc
     4321 cagatagccg ttgcccgacc gaccagattc gcttcaggca caaaacccca gtaacggctg
     4381 tccgcgctgt tgtcgcggtt gtcgcccatc atgaagtatt gtcccggcgg aacaatccag
     4441 gttgccagtt gttgccctgg ctgctggtaa tacatcccca cctggtcctg cgcaatcggc
     4501 actgtcagaa tgcggtgcgt cacatcaccc aatgtctctt tacgctcgga aagacgaatt
     4561 ccattttctt tggtttcgtt tttcggcact tcaaagaatc cgctggtcgc ttccccacca
     4621 ttacggcgtg agaaggtctg aacgaaatcg ctcggttcca cgttggagta agtgaccggt
     4681 agcgcatttt cacacgcctg gccggaactg catcccggtt gaatcgtcag ctcttttgag
     4741 accggatcat aagtgacttt atcgcccggc aaacccaccg cgcgcttgat gtaatcaagc
     4801 tttggatctt ccggatattt aaagaccacg atatcgccgc gtttcggatg accggtttcg
     4861 atcagcgttt tctggtagat aggatcttta atgccataag caaacttctc taccagaata
     4921 aaatcaccga ttaacagagt cggcatcatc gaacctgacg ggatctggaa cggttcataa
     4981 ataaacgaac gcacaatcaa tacgatagcc agtaccggaa aaacagaagc accggtttcc
     5041 agccagccag gcttcggcgc aacctttttc agcgttgctt tatccagtga gtcaccggca
     5101 gccgcctgcg ccgctgcctg acgttcccgc cgtttaggtg cgaaaaagaa tttatccacg
     5161 caccataaaa tgcccgtcac cagtgtggca atcaccagaa tcagggcaaa catattcgcc
     5221 atgccaactc ctaagggtta tttgttgtct ttgccgacgt gcagaatggc gaggaacgct
     5281 tcctgcggca gctcgacgtt accgatctgc ttcatgcgtt tcttaccttc tttctgcttc
     5341 tgcagcagct ttttcttacg gctgatatcg ccgccataac atttagccag tacgttttta
     5401 cgcagctgtt tcacggtgga tcgcgcaata atgtgcgtac caatcgctgc ctgaatggcg
     5461 atatcaaact gctggcgtgg gatcagatct ttcatcttct ccaccagctc gcgaccgcgg
     5521 ttttgcgaat taccacggtg ggtgatcagc gccaacgcat caacacgttc accgttgatt
     5581 aatacgtcta cacgtaccat gtcggacgcc tggaagcgtt tgaagttgta atccagagac
     5641 gcataaccac gcgaggtaga tttcaggcga tcgaagaagt cgagcaccac ttccgccatc
     5701 gggatctcgt acgtcagcgc cacctgatta ccgtggtaaa ccatattggt ctgcacgccg
     5761 cgtttttcta cgcacagcgt aataacgttg ccgagatatg cctgcggtag cagcatgtga
     5821 cactctgcaa tcggctcgcg cagttcgtaa atgttattta ccgctggcag cttggatggg
     5881 ctgtcgacgt agataacttc tcttgacgtg gtttcaactt catacactac agtcggcgca
     5941 gtggtgatca gatccagatc gtattcacgt tccagacgtt cctggatgat ctccatgtgc
     6001 agcaggccaa ggaagccgca gcggaaacca aagcccagcg cgcttgagct ttccggctca
     6061 tagaacagtg aggcatcgtt caggctgagt ttacccagcg cgtcacggaa ggcttcatag
     6121 tcgtcggaac ttaccgggaa cagaccagca tatacctgag gtttgacttt cttaaagcca
     6181 ggcagcgcct tttctgccgg attacgcgcc agtgttaagg tatcgcctac tggagcgccg
     6241 tggatatctt taatcgcaca taccagccag cctacttcgc cacatttcag ttcagtacgg
     6301 tcaacctgct tcggcgtgaa gatgcccaga cgatcggcgt tataggtctg cccggtgctc
     6361 atgactttca ctttgtcgcc cttacgcagg gtgccgtttt taatacggat aagtgaaaca
     6421 acgcccagat agttgtcgaa ccaggagtcg ataattagtg cctgcaacgg gccttccgga
     6481 tcgccttccg gcggcggaat gtcgcgcaca agacgttcga gaacgtcctg cacgccaacg
     6541 ccggttttcg ctgaacagcg caccgcgtcg gtggcgtcga tgccgacgat atcttcaatt
     6601 tcttccgcca cgcgttcagg atcggctgcc ggcaggtcaa tcttgttcag taccggaacg
     6661 acttcgagat ccatttccat ggcggtgtag cagtttgcca gggtttgcgc ttcaacgccc
     6721 tgcccggcat cgaccaccag cagtgcgcct tcacaggcag ccagcgaacg ggaaacctca
     6781 taggagaagt ctacgtggcc cggggtgtcg ataaagttaa gctgataggt ttcgccatca
     6841 gacgctttgt agtccagcgt cacgctttgc gctttgatgg taatgccacg ctcacgctca
     6901 agatccatgg aatcgagaac ctgcgcctcc atttcacggt cagacaggcc accgcagatc
     6961 tggataatac ggtcagacag cgtcgattta ccgtggtcaa tgtgagctat gatcgaaaag
     7021 ttacgtatat tcttcataaa gtatgattat tgtgccttaa tgcccggtta accaggcttt
     7081 tagaagtcgc tgttctgagc ttaacgtctg tattaataga aacgccgcat tctacactac
     7141 aacattgagg cgaggaaatg ttcataccgt atggattgtg gtatctggaa acgtcctcgc
     7201 atttgttatg caaaatgcaa caaagccagt gaaatcactg gctcgcgtct tccgaagatg
     7261 tttcaaatcg cacaaggcca ggcggcaatg ccacgcttaa gatgatcggt tgccattctg
     7321 cccgggcagc aaacttacgc gaatagccgc gcgcaatcag gaacccgcca ataccgccga
     7381 ggatcgcacc acataatgct gcgacgtcag aagcaaagag tagctgaaat agcgaagcga
     7441 tgaggaataa tcccaccagc ggcgacatat aaaccagtaa tgcggagcta agcaggctgc
     7501 cttcggcgat ccccaattcc actttttgcc ccggcactaa cggctcatca caggatacga
     7561 caatggtatg cgtggtttgc gggccaagtt tattcaacac acggctaccg caaccggcgc
     7621 gtgaagcgca gctgctgcat gaggctttaa catcacaact gaccagcgcc tgcccgtttt
     7681 cccaggagac gacggtagcc cactctttga tcattgcgct gtcccgaact taatattctc
     7741 ggcaatgcgt ttcgccgttt gcggcggcag ttcaccaaca atggtgattt cggcgttatc
     7801 acgtacgctt gtactgacgg ttctgcgtcc ggtgcgcaac atctgatcgg tgctcgatgg
     7861 cgtagcgcgg ttaacgttta ccgagaagct gaataatccg tcggaataga gacgtgattc
     7921 gataggcatg ttgtccatcg tcggtagcgg acgtcgacta ctggaaactt cgctaaaacc
     7981 ctgtggcagc caggttggcg tccagctgaa tttagctttt tcaccgacag gaacagaaag
     8041 caacggcggc aaatttgcct tcgccagcgt ctgcatactg ctgccgatat cctgattgac
     8101 gttaaaagca atcacgcgaa attgttccag cgtttcacca tcgcgatcaa ggagatcaac
     8161 ccgcatcggt aatttcgatt cggtgtccat ccacacgatg tagctgtagc gtgtaccatc
     8221 tcgggcaacc acgcgaatga cttcgcaaag acgatcagca atacgcgtgc gcccgacgga
     8281 gataaagtcg tagtaaggag aaaggcgttt gaaatcggta tagatgagcg atggcagaga
     8341 atcaacaatg taatcgccat taagcgtgaa cggttcaagt cccggttcaa aatagctgat
     8401 ttcattgccg cgctgtacca cttcccggcg cgggccatcc atttgcaaca attgtgcaag
     8461 aggacggtta tcgaggcgtg catgtcgata acgcagagac tcaacaccct gtttattgat
     8521 gctgatgaat gacagctcgt aattcagtga ctgactggcc aggttcatct gctgtaataa
     8581 cgcctcggac gcgggagtgg ccgaggcgtt agcagagaat aacaggctac ctgtcactaa
     8641 tgacatggca aaccaaagtt gcttcattac tgcgattgcg ttcctaaagt ttgaattcct
     8701 ggcacctgta cagcggcttg ctgagtttgt gcctgctcaa actgaagctg ttcagagtgg
     8761 agtcggcgtt gcagttcgta atcctgcaac attgcattaa tgcgacgacg ctgctcctgt
     8821 acctgctgct gttgaccatt atttgcggtc gcttcagaag gtactcccag actaaccggg
     8881 ctggctttac ccatcatcgg cagtgtatta aatactggcg tttcgggctg ctgggacgtt
     8941 tcagattgcc cattatagtg ctggacgcca acgataactg caagcgatac gcatgctgct
     9001 acgcccattt gggtaagctg tgccgcccac ggacgtactt tctgccagaa tggcattttc
     9061 tgccattgat gcggcgcagg ctgggcttcc gggatcaatg tcgccggttg acgtactggc
     9121 tcttcttcaa tggcggccat cacacgtgaa gagatatcga aatggagcac ctcgggagta
     9181 tcaccccgca ttgagtcacg gatcaagtga tagctttccc aggttttctg catttctggg
     9241 ttatgagcca gttcgttaag cagctcacta tccagcgttt cgccatccat taaagcggaa
     9301 agttgttctt tctgcatgcc taataccctt atccagtatc ccgctatcgt caacgcctga
     9361 taagcggttg aactttgtta tcaatagctt ccctcgctcg gaagatacgt gaacgcaccg
     9421 tacctaccgg acaatccatg atagcggcta tctcttcata gctcaggcca tccagctccc
     9481 gcaaggttat tgccatgcgt aaatcttccg ggagggactc aatagttcgg aaaactatct
     9541 gtctcagttc ttctgacaac attaaattct cagggttcga aatttctttc aacgcgccgc
     9601 cactttcgaa gttttcagct tcaatggcat ccacatcact ggaaggtgga cgacgcccct
     9661 gagcaaccag gtaatttttc gctgtattta cagcaatccg atacagccat gtataaaaag
     9721 cgctatctcc ccggaacgaa tccagcgcac gataggcttt aataaaagct tcttgtacca
     9781 catcgggaac atcacccgac ggcacatagc gggaaaccag actcgccact ttatgctgat
     9841 agcgcactac cagtaagtta aaggctttct gatctccctt ctggacccgt tcaaccagga
     9901 cctggtccgt taactgctcg ctcatccgag gtaaagtctc cccaaaccaa atttccacgc
     9961 gctatcgaaa cgccactcca ttagctgcaa tttgagcaag caaagggtta gagtgtctcg
    10021 tttttgtaaa gttccgtaac gcatctgttt ttgtttgtca tgctgtagac ggatcattat
    10081 ctatcattat aagtctacag aatctgaaca tcgcattatc tgtgtagaaa tgcccattta
    10141 actgcctgaa gagtaaccca acggcctttt tatttcacca cctaatcctc caccagccag
    10201 taacttctcg ttttctcgcc gccctgaggc agcgtgttta gtcactgtaa caaatattaa
    10261 aatagcagat gtttattcgc acaacatgat gttatgctga ccaaaccgtg tttagtaaat
    10321 taaacaaaga aaatgaatac tctccctgaa cattcatgtg acgtgttgat tatcggtagc
    10381 ggcgcagccg gactttcact ggcgctgcgc ctggctgacc agcatcaggt catcgttcta
    10441 agtaaaggcc cggtaacgga aggttcaaca ttttatgccc agggcggtat tgccgccgtg
    10501 tttgatgaaa ctgacagcat tgactcgcat gtggaagaca cattgattgc cggtgctggt
    10561 atttgcgatc gccatgcagt tgaatttgtc gccagtaatg cacgatcctg tgtgcaatgg
    10621 ctaatcgacc agggggtgtt gtttgatacc cacattcaac cgaatggcgc agaaagctac
    10681 catctgaccc gtgaaggtgg acatagtcac cgtcgaattc ttcatgccgc cgacgccacc
    10741 ggtagagaag tagaaaccac gctggtgagc aaggcgctga accatccgaa tattcgcgtg
    10801 ctggagcgca gcaacgcggt tgatctgatt gtttctgaca aaattggcct gccgggcacg
    10861 cgacgggttg ttggcgcgtg ggtatggaac cgaaataaag aaacggtgga aacctgccac
    10921 gcaaaagcag tggtactggc aaccggcggt gcgtcgaagg tttatcagta caccaccaat
    10981 ccggatattt cttctggcga tggcattgct atggcgtggc gcgcaggctg ccgggttgcc
    11041 aatctcgaat ttaatcagtt ccaccctacc gcgctgtatc acccacaggc acgcaatttc
    11101 ctgttaacag aagcactgcg cggcgaaggc gcttatctca agcgcccgga tggcacgcgt
    11161 tttatgcccg attttgatga gcgcggcgaa ctggccccgc gcgatattgt cgcccgcgcc
    11221 attgaccatg aaatgaaacg cctcggcgcg gattgtatgt tcctcgacat cagccataag
    11281 cccgccgatt ttattcgcca gcatttcccg atgatttatg aaaagttatt gggactgggg
    11341 attgatctca cgaaaggccc tgtgccgatt gtgcctgctg cacactatac ctgcggtggt
    11401 gtaatggttg atgactatgg gcgtaccgac gttgatggtc tgtacgccat cggcgaagtg
    11461 agctacacgg gattacacgg cgcgaaccgt atggcctcta actccttgct ggagtgtctg
    11521 gtctatggct ggtcggcggc agaagatatc accagatgta tgccttatgc ccacgacgtc
    11581 agtacgttac cgccgtggga tgaaagccgt gttgagaacc ctgacgaacg ggtggtgatt
    11641 caacataact ggcacgagct acgtctgttt atgtgggatt acgttggcat tgtgcgcaca
    11701 acgaagcgcc tggaacgcgc cctgcggcgg ataaccatgc tccaacaaga aatagacgaa
    11761 tattacgccc atttccgcgt ctcaaataat ttgctggagc tgcgtaatct ggtacaggtt
    11821 gccgagttga ttgttcgctg tgcgatgatg cgcaaggaga gtcgggggtt gcatttcact
    11881 ctggattatc cggaactgct cacccattcc ggtccgtcga tcctttctcc cggcaatcat
    11941 tacataaaca gataaaaagc ctgggtcagc gccgtatacg cttcggaata gttctggtct
    12001 ggcccacgaa tgactaagcg atcgctaaag cattctcccg cctgcgggga gaatgccagc
    12061 agcacccgat gcggcagtcg cgcttcgttt tccgccacat ccgtccgcaa acgtaaatgc
    12121 cagcccatgc ttaatgccag ttcagtaaaa ccattaccaa tctgctctgg cagcactacg
    12181 cagaaaaatc cctcttcggt aatgcactcc gccgcacagg tcagcagcga tgggtgatca
    12241 agcgtagtgg tatagcgagc ctgttcccgt tgaggtgtcg cgcactctac tccctgctga
    12301 tagtaaggtg ggttactgat gattaaatcg aagcgtactg tctgctgtgt gatccactgc
    12361 tgaatatccg ccgtatggac gttaatccgc tctgcccacg gggactggta gatattttcc
    12421 tgcgcctgcg cggcagcttc actttccagt tcaactgcat caatcattac gctgtcatcg
    12481 gttcgctgcg ccagcattaa tgccagcaac ccgctacccg cgccgatatc aaggcaacgt
    12541 tttaccccag ccaccggtgc ccatgcgccc agcaaaatgc catccgttcc cactttcatc
    12601 gcacaacgat cgtgagcaac aaaaaactgt ttaaaagtaa atccattacg acgaaacacg
    12661 gatgtagact gtgacatgaa aataaaacct tgcaggaaaa acggcgatag caccgggtga
    12721 gaacaatacc cgagaagcga tatccataca aacagatgaa gattgcagcc gtaacgtcta
    12781 taatcagcgc cccacacaga ggtagaacat gactgtaacg actttttccg aacttgaact
    12841 cgacgaaagc ctgctggaag ccctccagga taaaggtttc actcgcccga ccgccattca
    12901 ggctgccgcc attccgcctg cgctcgatgg ccgtgatgta ctcggttctg cgccgacagg
    12961 caccggtaaa acggcggcgt atctgctgcc agcgttgcag cacctgctcg atttcccacg
    13021 taagaaatcc ggtccgccgc gtattttgat cctcacccca actcgcgagc tggcgatgca
    13081 ggtagccgat catgcccgcg aactggcgaa acatacgcat ctggatatcg ccaccatcac
    13141 cggcggcgta gcctatatga accacgcgga agtgttcagc gaaaatcagg acatcgtggt
    13201 cgccacgacc ggacgtctgc tgcaatacat aaaagaagag aacttcgatt gccgcgcggt
    13261 tgaaacgctg atcctcgacg aagcagaccg tatgctggat atgggcttcg ctcaggatat
    13321 cgaacatatt gctggcgaaa cgcgctggcg taaacagacc ctgctctttt cggcaacgct
    13381 ggaaggcgat gcgattcagg actttgccga gcgtctgctg aaagatccgg tggaggtttc
    13441 tgccaatccg tccacccgtg agcgcaaaaa aattcatcag tggtattacc gcgccgatga
    13501 cctggaacat aaaaccgcat tgctggtgca cctgttaaaa cagccggaag cgacccgctc
    13561 aattgtgttt gtgcgtaagc gtgagcgtgt gcatgagctg gcaaactggc tgcgcgaagc
    13621 gggcatcaac aactgctatc tcgaaggtga gatggtacaa ggcaagcgta acgaagcgat
    13681 caagcgtttg accgaaggtc gcgtaaacgt actggttgca accgatgttg ccgcgcgcgg
    13741 tatcgacatt cctgacgtca gccacgtctt taacttcgat atgccgcgca gtggcgatac
    13801 ttatttgcac cgtatcggac gtaccgcgcg cgccggtcgt aaaggcaccg caatttcgct
    13861 ggtagaagcc catgaccatc tgctgctggg caaagttggt cgctatattg aagagccaat
    13921 taaagctcgt gtaattgatg agttacgccc gaaaacgcgt gcgccaagcg agaagcagac
    13981 cggcaagcca tcgaagaaag tactggcaaa acgcgccgag aagaaaaaag ctaaagagaa
    14041 agagaagccg cgggtgaaaa aacgccatcg cgacaccaaa aatattggta agcgccgtaa
    14101 accgagcgga acgggcgtgc caccgcaaac gacagaagag taatctcaat gccgggttta
    14161 agcccggcat tatttttatc ccggcaaaaa acactcttca acacactgaa taaaaatgtg
    14221 catcgccggg ctaaccgctt ttccggcatg gtgagcgcac attgccgtaa tggtctgcga
    14281 ctgttcgcca aaaggcaatt cgattaattc gccactcgca agttcatttg ccactgtaaa
    14341 gcgcggcagg taactgaccc cgatatttgc cgcgacacaa cgcttgatgc tttcgatact
    14401 tataagctca atggtgtttt ccaccgtgat ccgtcgctgg cgcagcgtgc tctcaaatat
    14461 ctggcggaag acacattgcg gttcattgat aataaaacta caggcgttat gtcttcccgg
    14521 ctcagtaaaa tcgacatctg caatttgcgg tgaagctacc agcaccagca attgttcacc
    14581 caactctcgt cggttcagag catcatcatt ccctacgcgg taaaagacgc cgacatcagc
    14641 ctcatcattc agtagcgcat cacggatcac gtaacagttc agagactgca acgataaacg
    14701 cacttttggg gcgcgttgcc gaaaacgctg caacacctgc ggcattcggt aagagagcaa
    14761 cgtttcgcct gaaacaacgc gtagttcccc gtccggatcc gactcttttt tcgcagcctc
    14821 gcgtagcgta tccattactc tggtgagttc atagatgtgc ggcagcaact ttttgccttc
    14881 gcgagtgagg cacatccgtc ggccaatttt ctcaaacaat tgtaccgaga actcctgctc
    14941 aagctgctga atatgaaaag tcaccgtcga ttgtgtacag cacaattttt gcgaagctcg
    15001 caaaaaggaa ccctcttcca ccacggtttt aagtgtaata aaacggcgca gatccataac
    15061 cccaaa
//
LOCUS       DAZGJT010000105        14976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZGJT010000105 DAZGJT010000000
VERSION     DAZGJT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.105"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..126
                     /locus_tag="ACHKFK_003800"
     CDS             <1..126
                     /locus_tag="ACHKFK_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054044.1"
                     /translation="DTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHIIG"
     gene            144..542
                     /gene="yjfL"
                     /locus_tag="ACHKFK_003801"
     CDS             144..542
                     /gene="yjfL"
                     /locus_tag="ACHKFK_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO9054045.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAAALAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            552..1190
                     /gene="yjfM"
                     /locus_tag="ACHKFK_003802"
     CDS             552..1190
                     /gene="yjfM"
                     /locus_tag="ACHKFK_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO9054046.1"
                     /translation="MARKRKSRNNKNIGHGAISRIGRPHNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYATVQDCIDDGNNSDICAHGWNNAKAAFYADV
                     PKNMTQHNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            1193..2356
                     /gene="yjfC"
                     /locus_tag="ACHKFK_003803"
     CDS             1193..2356
                     /gene="yjfC"
                     /locus_tag="ACHKFK_003803"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO9054047.1"
                     /translation="MLRHNVPVRRDLDRIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGVIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFDGEKPQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2440..4065
                     /gene="aidB"
                     /locus_tag="ACHKFK_003804"
     CDS             2440..4065
                     /gene="aidB"
                     /locus_tag="ACHKFK_003804"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO9054048.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGASWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTSAERLDDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGIPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCKVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(4182..4457)
                     /gene="yjfN"
                     /locus_tag="ACHKFK_003805"
     CDS             complement(4182..4457)
                     /gene="yjfN"
                     /locus_tag="ACHKFK_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO9054049.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(4606..4935)
                     /gene="bsmA"
                     /locus_tag="ACHKFK_003806"
     CDS             complement(4606..4935)
                     /gene="bsmA"
                     /locus_tag="ACHKFK_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO9054050.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            5117..5866
                     /gene="yjfP"
                     /locus_tag="ACHKFK_003807"
     CDS             5117..5866
                     /gene="yjfP"
                     /locus_tag="ACHKFK_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO9054051.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFSVALAQAGLRVIMPDAPDHGSRFSGDAAWRLKQFWQILLQSMQEFTTLRAAIAEEN
                     WLRDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFHQHL"
     gene            complement(5863..6618)
                     /gene="ulaR"
                     /locus_tag="ACHKFK_003808"
     CDS             complement(5863..6618)
                     /gene="ulaR"
                     /locus_tag="ACHKFK_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO9054052.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6726..7790)
                     /gene="ulaG"
                     /locus_tag="ACHKFK_003809"
     CDS             complement(6726..7790)
                     /gene="ulaG"
                     /locus_tag="ACHKFK_003809"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO9054053.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGANVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GTLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADILRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            8145..9542
                     /gene="ulaA"
                     /locus_tag="ACHKFK_003810"
     CDS             8145..9542
                     /gene="ulaA"
                     /locus_tag="ACHKFK_003810"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO9054054.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIERMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            9558..9863
                     /gene="ulaB"
                     /locus_tag="ACHKFK_003811"
     CDS             9558..9863
                     /gene="ulaB"
                     /locus_tag="ACHKFK_003811"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO9054055.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            9873..10337
                     /gene="ulaC"
                     /locus_tag="ACHKFK_003812"
     CDS             9873..10337
                     /gene="ulaC"
                     /locus_tag="ACHKFK_003812"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO9054056.1"
                     /translation="MKLRDSLAENKSIRLQTEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            10351..11001
                     /gene="ulaD"
                     /locus_tag="ACHKFK_003813"
     CDS             10351..11001
                     /gene="ulaD"
                     /locus_tag="ACHKFK_003813"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO9054057.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIGQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            11011..11865
                     /gene="ulaE"
                     /locus_tag="ACHKFK_003814"
     CDS             11011..11865
                     /gene="ulaE"
                     /locus_tag="ACHKFK_003814"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO9054058.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     DRLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            11865..12551
                     /gene="ulaF"
                     /locus_tag="ACHKFK_003815"
     CDS             11865..12551
                     /gene="ulaF"
                     /locus_tag="ACHKFK_003815"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO9054059.1"
                     /translation="MQKLKQQVFEANMDLPHYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(12681..12956)
                     /gene="yjfY"
                     /locus_tag="ACHKFK_003816"
     CDS             complement(12681..12956)
                     /gene="yjfY"
                     /locus_tag="ACHKFK_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO9054060.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            13284..13679
                     /gene="rpsF"
                     /locus_tag="ACHKFK_003817"
     CDS             13284..13679
                     /gene="rpsF"
                     /locus_tag="ACHKFK_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO9054061.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13686..14000
                     /gene="priB"
                     /locus_tag="ACHKFK_003818"
     CDS             13686..14000
                     /gene="priB"
                     /locus_tag="ACHKFK_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO9054062.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14005..14232
                     /gene="rpsR"
                     /locus_tag="ACHKFK_003819"
     CDS             14005..14232
                     /gene="rpsR"
                     /locus_tag="ACHKFK_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO9054063.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14274..14723
                     /gene="rplI"
                     /locus_tag="ACHKFK_003820"
     CDS             14274..14723
                     /gene="rplI"
                     /locus_tag="ACHKFK_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO9054064.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
BASE COUNT         3515 a         3645 c         4019 g         3797 t
ORIGIN      
        1 gatacttacg aatatttgct gttaaatggt gaggaatctt ttaacgatct cggcgagccg
       61 gagtggttat tttcgcgcgc gctgggggtc gatatcccgc tgacatcact tcatattatt
      121 ggttaattac aaggacgttt acaatgcata tactggattc acttcttgcc tttagtgcct
      181 atttttttat tggcgtggcg atggtgatta ttttcctgtt tatctactcc aaaattacac
      241 cgcacaacga atggcagtta atcaaaaaca ataacaccgc ggcggcactg gcgttcagcg
      301 gaacattgtt ggggtacgtg atccccttat ccagtgcggc aatcaatgcg gtgagtattc
      361 cagactattt cgcatggggc gggattgctc tggtgattca attactcgtt tttgctggcg
      421 tcaggcttta tatgcccgca ttaagcgaaa aaattattaa tcacaatacc gcagcaggaa
      481 tgtttatggg aaccgccgcg ctggctggcg gtatttttaa tgcagcttgt atgacatggt
      541 aatggacgat catggccaga aaacgcaaat ccagaaataa caaaaatatc ggacatgggg
      601 caatcagtcg cattggtaga ccgcataatc cttttgagcc gcgccgcaat cgctacgcac
      661 aaaaatactt aacgttggcg ctaatgggcg gtgccgcttt tttcgtattg aaaggttgtg
      721 gcgatagtgg cgatgtcgat aacgacggcg acggaacgtt ttacgcgact gtgcaggatt
      781 gtattgatga tggtaataat tcagatatct gtgctcatgg ctggaacaac gccaaagcgg
      841 cattttatgc cgatgttccg aagaatatga ctcagcataa ttgtcagtct aagtacgaaa
      901 attgctacta cgacaatgtt gaacagagtt ggatcccggt cgtttctgga tttttgttaa
      961 gccgggttat tcgtaaagat cgcgatgagc cgttcgtcta taacagcggc ggatcctcat
     1021 ttgcttcccg ccccgtctgg cgcaacactt ccggtgatta ctcctggcgc tccggttctg
     1081 gcaaaaaaga gtcttactct tcgggcggct ttaccaccaa aaaagcgtca accgtttctc
     1141 gcggcggcta tggtcgttct tccagcgccc gtgggcattg gggaggctaa tcatgctgag
     1201 acacaacgtt cctgtgcggc gggatctgga ccggattgcc gccgataacg gatttgactt
     1261 tcatatcatc gacaatgaaa tctattggga tgagagtcgg gcttatcgtt ttactctgcg
     1321 ccagattgaa gagcagatcg aaaaaccgac tgcggaactg catcagatgt gcctggaggt
     1381 ggtggatcgc gcggttaaag atgaagagat cctgacgcaa ctggcgatcc cgccgttgta
     1441 ctgggatgtg atcgccgaaa gctggcgcgc acgcgatcct tcgttgtatg gccggatgga
     1501 ttttgcctgg tgtggtaatg caccggttaa gttgctggag tacaacgccg atacgccaac
     1561 ttcattgtac gagtcggctt atttccagtg gttgtggctg gaagatgccc ggcgcagcgg
     1621 cgttattccg cgtgatgccg atcagtacaa tgccattcag gaacgcctga tttcacgttt
     1681 tagcgagctt tacagtcggg aaccgtttta tttttgctgc tgtcaggaca ccgacgaaga
     1741 caggagtacc gtgctgtact tgcaggactg cgcccagcag gcagggcagg agtcgcggtt
     1801 tatctacatt gaagatctcg gtttgggcgt cggcggcgta ctgaccgatc ttgatgataa
     1861 tgtcatccag cgtgcattta agctgtatcc gctggagtgg atgatgcgtg acgataacgg
     1921 cccgctgctg cgcaagcgtc gtgagcaatg ggtggagccg ttatggaaaa gtatcttgag
     1981 taataaaggg ctaatgccgc tgctttggcg cttcttccct ggtcatccta accttcttgc
     2041 atcctggttc gatggtgaaa aaccgcagat tgccgctggc gaaagctatg tgcgtaaacc
     2101 gatttactcg cgcgaaggcg gaaacgtcac cattttcgac ggtcagaata acgtcgttga
     2161 ccacgctgat ggtgattacg ccgatgaacc gatgatctac caggcgtttc aacctctgcc
     2221 gcgatttggc gatagctaca cactcatcgg tagctggatt gtcgatgatg aagcgtgcgg
     2281 aatggggatc cgtgaagata acacgctgat caccaaagac acctcacgtt tcgttccgca
     2341 ttacattgct ggataagaat gttttagcaa tctctttctg tcatgaatcc atggcagtga
     2401 ccatactaat ggtgactgcc attgatggag ggagacacag tgcactggca aactcacacc
     2461 gtttttaatc aacctatacc attaaataac agcaacttat acctgtctga tggcgcgctt
     2521 tgcgaagcgg taacgcgtga aggtgctagc tgggatagcg attttctcgc cagtattggt
     2581 cagcagttag gaacggctga atcccttgaa ctggggcggc tggcgaatgt gaatccgcct
     2641 gaattattgc gctacgatgc gcaaggacgc cgtctggacg atgtgcgttt tcaccccgcc
     2701 tggcacctgc tgatgcaggc gctatgtacc aatcgggtgc acaatcttgc ctgggaagaa
     2761 gacgctcgct ccggcgcatt tgtggcgcgc gcggcgcgtt ttatgttaca tgcgcaggtt
     2821 gaggctgggt cgttatgtcc gataacgatg acctttgccg ccacgccatt gttgttacag
     2881 atgttacccg cgccgtttca ggactggacc acgccgctgc tgagcgatcg ctacgattct
     2941 cacttattgc caggtgggca aaaacgtggc ttgttgatcg gcatgggaat gacggaaaag
     3001 cagggcggtt ccgatgtcat gagcaacacc accagcgcag agcgtctgga cgatggttct
     3061 tatcggctgg tggggcataa atggtttttc tcggtgccgc aaagcgatgc gcatctggtg
     3121 ctggcacaga ccgcgggcgg tttgtcctgc ttttttgtgc cgcgcttttt gcctgacggg
     3181 caacgcaacg cgattcgcct cgagcggctg aaagataagc tgggtaatcg ctctaacgcc
     3241 agctgcgaag tggagtttca ggatgccatt ggctggttgt tggggcagga aggggaagga
     3301 attcgtctga tcctgaaaat gggtgggatg acgcgttttg attgcgccct gggtagccat
     3361 gccatgatgc gccgtgcatt ttcgctggcg atttaccatg cacatcaacg ccatgttttt
     3421 ggtaatccat tgatccaaca gcctcttatg cgtcatgtct taagtcgcat ggcacttcag
     3481 cttgaagggc aaacggcgtt gctgtttcgt cttgcgcgag cgtgggaccg gcgcgccgat
     3541 gccaaagaag ctctgtgggc gcgtttattt acgcctgcgg cgaaatttgt gatctgcaaa
     3601 cgaggcattc cgtttgtggc cgaagcgatg gaggtgctgg gcggcattgg ttattgcgag
     3661 gagagcgagc tgccgcggct ttaccgggag atgccggtaa acagtatttg ggaaggttcc
     3721 ggcaatatta tgtgcctgga tgtgctgcgc gttctcaata agcaagcggg cgtatacgac
     3781 ttattgtcgg aagcatttgt ggaagtgaaa gggcaggatc gctattttga tcgcgcggtt
     3841 cgtcgtttac agcagcagct gcgtaagcca gctgaagaat tggggcgaga gattactcat
     3901 cagctattcc tgctgggctg cggtgcgcaa atgttgaaat atgcctctcc gccaatggcg
     3961 caggcgtggt gtaaggtgat gttagatacg cgcggcggcg tacggttgtc agagcagatc
     4021 cagaatgatt tattgctgcg ggcgacggga ggagtgtgtt tgtaagcgta tacgactgat
     4081 gcgacgctgg tttcgattaa ctaaatgaaa tattttaaaa ttgtaggccg gacaaggcgc
     4141 tcgcgccgca tccggcattg ttcatttgct gtagatgaca ctcacgcata cagtatcgcc
     4201 tgtacgcgcc agttatcagg ctgctggtct tcgtacatgg taataatgcg gtaccatgac
     4261 gcaccttgtt catcggcctt taacgccacg atacgttcca cgtcctgcgg atcccctgaa
     4321 atattgctaa ctgagatctg acctatttca ttcaggcccg tcacgcagtc agcactggcg
     4381 aattctgcag actgtgccgt ggcgctcgca ggataagtca ctaattgcag cgagggtgag
     4441 gcaagtaatt gtttcatcgt cagctccatt tttcgtttcc ccacatgttg cagggcattc
     4501 catcacggta ataaacgccc gagcttccag ccggtaattt attgtgcaca ggtaaacgca
     4561 tagggacgca aagcaatgta aagctgacta tgtaagttgt ctgatttatt tacggtacaa
     4621 aatggcttgt gaataccact gtcctgtcac gatggtttcg tccaccatga cgacaacgta
     4681 ataatcagct tttgcagcga cagctttcgc tctgatttct gctaatgcgt catccggaga
     4741 accccgaacc atcgtgctta cgctacctat tcgctgtaac ccttgcgtct ggtcgcggcg
     4801 aatctcttgc ggatggtccg ttactggcgg tgctggctgt ggcgtacctt gcagtgcgct
     4861 acaggcactt aacatcaaca ccaataataa actggcaaac cggtaaataa cgctattacg
     4921 tttcctgcta accatagtgt agtgccttat taatttaact ttgggggaat gttcccgaat
     4981 tgttacctgg tacgcgattt tcgaatgaaa acgtgacgaa agcgtaaccc acatctcaac
     5041 attatgaact aataccgcca gcagaaactt gtgctaagga tttcttcatg tcataccaga
     5101 caaaaaggag agacagatga ttgaaataga atcacgcgag ctggcagata ttcccgttct
     5161 tcatgcttat cctgtcgggc aaaaagatac cccgttaccg tgtgtgattt tttatcacgg
     5221 ctttacttcg tccagtctgg tgtatagcta tttttccgtt gcgctggcgc aggctggttt
     5281 gcgggtgatc atgccggatg cgcccgatca cggtagccgt tttagtggcg acgcagcgtg
     5341 gcggttaaag caattctggc aaatcttgct acaaagtatg caggaattca ctacattacg
     5401 tgcggcaata gccgaagaaa actggctgcg tgatgaccgt ctggcagtcg gtggcgcgtc
     5461 gatgggcgcg atgacggcac tgggaattac cgctcgccac cccacggtaa aatgtaccgc
     5521 cagcatgatg ggatcgggct attttacatc gctcgcccgt tcactgtttc caccgctgat
     5581 acctgaaacg gcagcacagc agaatgaatt caataacatt gtcgcgccgc tggcagagtg
     5641 ggaagcgaca aaccacctgg aacaacttgg tgacagacct ctactgctgt ggcatggcct
     5701 tgacgatgac gtcgtgcctg ccgacgaatc actacgtttg cagcaggcct taagcgagac
     5761 ggggcgggat aaactgctaa cctgttcatg gcagccaggc gtgcgtcacc gcattacgcc
     5821 tgaggcgtta gatgctgccg tgacattttt ccaccagcat ctttaaacac gcagaatgct
     5881 gaccccttgc gcttccagtt gttgcaggat ttccgggtta gcatttttgc cggtgatcag
     5941 catatcgatt tgatcggcac ggctaaaaag cattcccgcg cgttcgccaa tcttactgct
     6001 atcaaccagt accaccagtt tccctaccac gctcagcatc ttctgctctg ccattgccgt
     6061 cagcatatcg gttttataca acccttctgc tgtcagtcct ttgccgctgg taaacatcca
     6121 gtgcccggca tagagactgt tttcgctgcc ttgcgggctt aaagtgatgg actgactttt
     6181 gttgtactgt ccgcccataa tgatcacgct gtcatgctcc tgatcgatca ggtaatttgc
     6241 cagcggtaga taattagtga tgatttgcac tggcttgcca cacatttccc gcccaagcag
     6301 aaacgcggtg gagccgcagt tgatgactac gctttcgccc ggattaacca gctgcgaggc
     6361 cgctttagca atacgtactt tttcatcgtg attctgcgct tgatgcagat tcatcggcgt
     6421 ccagcgcggg cgctgttggg taatagcttc tgcgccattg cgcacttttt tcagtttgcc
     6481 gctttcgtca agtttattga tatcgcgtcg tgcagtggca ggcgaaattc ccagacgctc
     6541 aacgactttc tcaacggtca caaagcccaa ttgtgcgagc atttccagga ggatttgatg
     6601 tctttgtgct tcagtcatga gctattccga taaaatttga tttttttaga tgatatttga
     6661 aatagccagg aaatactatg ccggatagcg cgagcatctc cacgctatcc ggtacaagcg
     6721 acagattaca ggaacgactt gaacggcaga tctggttcaa tagtgaagca atcatcgaaa
     6781 ccacgcggat agtggtactc gaagttgtct ttatccagcg gccaggtaaa tttgccgccg
     6841 acctgccaga taaacggctt gaagccatac ttcaggcgat cttttttcat ctcccacagc
     6901 acgcggatct cttgcggatc ggcctggaag tttgaccaga tatcgtggtg gaacgggatc
     6961 actactttcg cattcagcgc ttcgcccata cgcaagatat cagcactggt cattttgtcg
     7021 gtaataccgc gtgggttttc gccgtatgaa cctaaagcca cgtcgatctg atgctcatta
     7081 ccatgttttg catagtagtt agagtagtgg gaatcgccgc tgtgatacag ggtaccgcct
     7141 ggggttttga acagatagtt caccgcgcga tcgtccatgc cgtccggcag tacgccagct
     7201 gctttttgat cggcaggcag ggtgatcagt gccgtgcggt cgaaagcgtc aagtgcatga
     7261 atttcaatgt cttttacctt cactacgtcg cccggtttaa cgacgatgca acgctctttc
     7321 ggtacgcccc agccaatcca caaatcaacg caggttttcg gtccaatgaa cggtacgtca
     7381 tcagcacagt tctgcatcac ggcagcagcg acgttaacgt cgatatgatc gttgtgatcg
     7441 tgagtcgcca gtaccgcgtc gatctggcga atcgcaaacg gatcaagaac aaacggggtg
     7501 gtgcgcaggt ttggctgcag ttttttcacg ccagccatgc gctgcatctg gtgaccctgt
     7561 ttcattaacg ggttaccgtg gctttgtttg ccagtaccgc accaaaaatc aacacaaacg
     7621 ttggcaccgc cttctgattt caaccagatc ccggtacagc cgagccacca cattgcgaaa
     7681 gtgccaggtg cgacctgctc ttgctcaatt tcttcattca gccagctacc ccactccggg
     7741 aaagtgctca ggatccagga ctcacgggtg atacttttca ctttactcat cgccatttac
     7801 cttcgtgata gttcaattcg aatcaatatg tgattggttt tgattaatcc tgacactatt
     7861 ttttcaggaa ggcaatgacc attttttgac tttttagcag gagagttatt gtggattttg
     7921 agtgtggaaa gattaaattg aatgtgtaat gcaattaact aaatgaattt aaatgaataa
     7981 atatttcatt atgtgaatcc cattctgttt atgtggaatt atttgcgtgc gacgtcacat
     8041 ttaatcataa ataatcttgt tgtgattact tttgaaaatt agagtgagag cacaacattc
     8101 cgggtgtgcg aaatacccgg ttacctcttc ttcaggagat cgttatggag atcctctaca
     8161 acatctttac cgtgtttttt aaccaggtca tgaccaatgc cccgttgttg ctgggtattg
     8221 tgacctgtct gggttacatc ctactgcgca aaagtgtcag cgttattatt aaaggtacga
     8281 ttaaaaccat aattggtttc atgttgttgc aggcagggtc cggcatcctg accagcacct
     8341 tcaaaccggt ggtggcgaaa atgtccgaag tctacggcat taacggcgca atttccgata
     8401 cttacgcgtc gatgatggca accattgaac gcatgggcga tgcctatagc tgggtgggtt
     8461 acgcggtgtt gttagcgctg gcgctgaaca tctgttacgt gctgctgcgc cgaattaccg
     8521 gtattcgcac catcatgctg actggtcaca tcatgttcca gcaggcgggg ctgattgccg
     8581 ttacgctgtt tatcttcggc tactccatgt ggacgaccat tatctgcacc gcgattctgg
     8641 tttcgctcta ctggggcatt acctccaaca tgatgtacaa gccgacacag gaagtcaccg
     8701 acggctgtgg tttctccatt ggtcaccagc aacagtttgc atcatggatt gcctataaag
     8761 ttgcgccgtt cctcggcaaa aaagaggaaa gcgttgaaga cctcaaactg ccaggctggc
     8821 tgaatatttt ccacgacaac atcgtctcca cagcgattgt catgactatc ttctttggtg
     8881 ccattctgct ctcctttggt atcgacaccg tgcaggcgat ggcaggcaaa gtgaactgga
     8941 cggtgtacat cctgcaaacc ggtttctcct ttgcggtggc gattttcatc atcactcagg
     9001 gcgtgcgcat gtttgtggcg gagctctctg aagcattcaa cggtatctcc cagcgcctga
     9061 tcccgggtgc ggttctggcg attgactgtg cggctattta tagcttcgcg ccgaacgccg
     9121 tggtctgggg ttttatgtgg ggcaccatcg ggcaactgat tgcggttggc atcctggtcg
     9181 cctgtggctc ttcaatcctg attattccag gctttatccc gatgttcttc tccaacgcca
     9241 ctatcggcgt gttcgctaac cacttcggcg gctggcgcgc ggcgctgaag atttgcctgg
     9301 tgatggggat gatcgaaatc tttggttgcg tctgggcggt gaaactcacc ggtatgagcg
     9361 cctggatggg catggcggac tggtcgattc tggcaccgcc gatgatgcag ggcttcttct
     9421 ccatcggcat cgcctttatg gccgtcatca ttgttattgc actggcttat atgttcttcg
     9481 ctggccgcgc actgcgtgca gaagaagatg cagaaaaaca actggcagaa caatctgctt
     9541 aataaggagt tttgattatg accgtacgta ttctggctgt gtgtggtaac ggacaaggca
     9601 gttccatgat catgaaaatg aaagtggacc agtttttaac ccagtcgaac atcgaccaca
     9661 cggtaaacag ctgcgcggtt ggcgaataca aaagcgagtt gagcggtgcg gatatcatca
     9721 tcgcttctac ccatattgcg ggcgaaatca ccgtgaccgg caacaaatat gtggttggcg
     9781 tgcgcaacat gctctctcct gctgattttg gcccgaaact gctggaagtg atcaaagagc
     9841 atttcccgca ggacgtgaag taaggacgcg ccatgaaatt acgtgattcg ctggctgaaa
     9901 ataaatctat ccgcctgcaa acggaagcag agacatggca ggacgcagtg aaaatcggcg
     9961 ttgatctgct ggtagcggca gatgtggtag agccgcgtta ctaccaggcg attctggatg
    10021 cggttgaaca gcacggcccg tactttgtgc tcgcacccgg cctggcaatg ccgcacggac
    10081 gcccggaaga gggggttaag aaaactggtt ttgcgctggt gacgctgaaa aagccgctgg
    10141 agttcaacca cgaagataac gacccggtgg atatcctcat caccatggcg gcggttgatg
    10201 ccaacactca ccaggaagtg ggcatcatgc agatcgtcaa cctgtttgaa gatgaagaga
    10261 atttcgaccg tttacgtgcc tgccgtaccg agcaggaagt actggatctc attgaccgca
    10321 ccaacgcggc agcttaagaa ggaattgaac atgtcattac cgatgttaca agtcgcgctg
    10381 gacaaccaga ctatggatag cgcctacgaa accactcgcc tgattgccga agaagtcgac
    10441 attatcgaag tgggcaccat tctttgcgta ggcgaaggcg tgcgtgcggt tcgtgacctg
    10501 aaagcgctgt acccgcacaa aatcgtactg gctgacgcca aaattgccga tgcaggcaaa
    10561 atcctttctc gcatgtgctt cgaagccaac gctgactggg tgaccgtaat ttgctgtgcg
    10621 gatatcaaca ccgccaaagg cgcgctggac gtggcaaaag agtttaatgg cgacgtgcag
    10681 atcgaactga ccggctactg gacctgggaa caggcgcagc agtggcgcga cgcaggcatt
    10741 gggcaggtgg tttatcaccg cagccgtgac gcgcaggccg caggcgtggc atggggcgaa
    10801 gcggacatta ccgcgatcaa acgtctttcc gatatgggct tcaaagtcac cgtcaccggt
    10861 ggtctggcgc tggaagatct gccgttgttt aaaggcattc cgattcatgt gtttatcgct
    10921 ggtcgcagca ttcgtgatgc cgcttctccg gtggaggccg cacgtcagtt caaacgttcc
    10981 atcgctgaac tgtggggcta aggagcggat atgttgtcca aacaaatccc gcttggcatc
    11041 tacgaaaaag cgctccccgc tggggagtgc tggctggaac gactgcaact ggcaaaaacg
    11101 ttaggcttcg attttgtcga gatgtcagta gatgaaacgg acgatcgcct ttcgcgcctc
    11161 gactggagcc gcgagcagcg tctggcactg gtgaatgcga ttgttgaaac cggcgtgcgc
    11221 gtaccgtcca tgtgcctttc tgctcatcgt cgtttcccgc tgggaagcga agatgacgcg
    11281 gtgcgggcgc aggggctgga gattatgcgt aaagctatcc agttcgccca ggacgtcggt
    11341 attcgcgtga tccaactggc gggctatgac gtttactatc aggaagccaa taacgaaacg
    11401 cgtcgtcgtt tccgtgacgg cctgaaagag agcgttgaga tggcgagccg cgcgcaggtc
    11461 acactggcga tggagatcat ggattaccca ttgatgaact ccatcagcaa ggcgctgggc
    11521 tacgcacact atctcaacaa tccgtggttc cagctctacc cggatatcgg caacctgtcg
    11581 gcgtgggata atgacgtgca gatggagttg caggctggaa tcgggcacat cgtcgcggtg
    11641 catgtgaaag acaccaaacc aggcgtcttc aaaaacgtgc cgtttggcga aggcgtagtg
    11701 gatttcgaac gttgcttcga aacgctcaaa cagagtggct attgcgggcc gtacctgatt
    11761 gagatgtgga gcgaaacggc ggaagacccg gcggcagaag tagtgaaagc gcgtgattgg
    11821 gtgaaagcgc gcatggccaa agcaggcatg gtggaggcgg cataatgcaa aagctgaaac
    11881 agcaggtgtt tgaagccaac atggatttac cgcattacgg gttggtgacc tttacctggg
    11941 gcaacgtcag cgctattgac cgtgaacgcg ggctggtggt gatcaagccc agcggtgtcg
    12001 cctacgaaac catgaaagcg gacgatatgg tggtggtcga tatgagcggt aaggtggtgg
    12061 aaggggagta tcgtccgtct tccgacactg cgacgcatct cgaactctac cgtcgttatc
    12121 cgtcgcttgg tggtattgtc catacccact ccactcatgc caccgcatgg gcgcaggcgg
    12181 ggctggcgat cccggcgtta ggcaccacgc acgccgatta cttctttggc gatatcccgt
    12241 gtacgcgtgg gttaagtgaa gaagaagtgc agggcgaata cgagctaaac actggcaaag
    12301 tgattatcga aacgctgggt gacgccgagc cgctgcatac gccgggaatt gtggtgtatc
    12361 agcacggacc gttcgcctgg gggaaagatg cccacgatgc ggtgcataac gcggtggtga
    12421 tggaagaagt ggcgaaaatg gcgtggattg cgcgcagtat taacccacaa ctgaatcaca
    12481 tcgacagctt tctgatgaat aaacacttca tgcgtaagca cggtcctaac gcttattacg
    12541 ggcagaagta aaacacgcgc tgcggaaatt tccttcttcg ggagataact ggtctaattc
    12601 cgctgccgtt tttcaaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
    12661 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
    12721 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtctct
    12781 tcatttaatg cctgacgtgc ttcactctca gtggcgaagt tatgattgat ataaatcacg
    12841 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
    12901 catgtatttg cacttagcaa aagcacagcc agaagggcta aaacgcgact gaacatagat
    12961 acctcctcga cggctgactt tgtgtgctct cctcttgttg tgatcttctg atttaatttt
    13021 aatcaatgat aaagaagttg atggtgacca tttctgatgc agttgttcaa aaaaacacca
    13081 tgatgaagcg tgatgaactc caaatcagcg atttagaggt tatttgcgaa aggggcgatt
    13141 tatttctgct cagcccttga gtttagcggg gtatgtcagt attataacgc gtcatttttc
    13201 agccgacctt taacacgttc cttgcctccc cgggattcgg ctgacccaga caggaggctg
    13261 aataatccgt aaggagcaat tcgatgcgtc attacgaaat cgtttttatg gtccatcctg
    13321 accagagcga acaggttccg ggcatgatcg agcgctacac tgctgccatc actggtgcag
    13381 aaggcaagat ccaccgtctg gaagactggg gccgccgtca gctggcttac ccgatcaaca
    13441 aactgcacaa agcacactac gttctgatga acgttgaagc tccgcaggaa gtgatcgatg
    13501 agctggaaac taccttccgc ttcaacgatg ccgttatccg cagcatggtt atgcgtacca
    13561 agcacgctgt taccgaagca tctccgatgg ttaaagcgaa agacgagcgc cgtgagcgtc
    13621 gcgatgattt cgcaaacgaa accgctgatg atgctgaagc tggggattct gaagagtaat
    13681 ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg caggactccc cttcgaaagg
    13741 tcagtccatc aggaattcct cactgccagt tcgtgcttga gcatcgttct gtgcaggagg
    13801 aagccggctt tcaccggcag gcatggtgcc aaatgcccgt tattgttagc ggacacgaaa
    13861 accaggccat tactcacagt ataacggtcg gcagtcgcat aaccgttcag gggttcattt
    13921 catgccataa ggcaaagaac ggactgagca aaatggtttt gcatgccgag cagattgaat
    13981 tgatagattc tggagactag ccatatggca cgttatttcc gtcgtcgcaa gttctgccgt
    14041 ttcaccgcgg aaggcgttca agagatcgac tataaagata tcgctacgct gaaaaactac
    14101 atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg gtacccgtgc aaaataccag
    14161 cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt ccctgctgcc gtacactgat
    14221 cgccatcagt aatcggtcac ggtccattaa tacgactttg agaggataag gtaatgcaag
    14281 ttattctgct tgataaagta gcaaacctgg gtagcctggg tgatcaggta aacgttaaag
    14341 cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc tgttccagct accaagaaaa
    14401 acattgaatt cttcgaagca cgtcgcgctg aactggaagc taaactggct gaagttctgg
    14461 cagcagctaa tgctcgcgct gagaaaatca atgcactgga aactgttacc atcgcgtcta
    14521 aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac tcgcgacatc gctgacgctg
    14581 taactgcagc tggcgttgaa gtggctaaga gcgaagttcg tctgccgaac ggcgttctgc
    14641 gtaccactgg cgaacacgaa gtgagcttcc aggttcacag cgaagtattc gcgaaagtga
    14701 tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga cgtaaaaacg ccgaccagtg
    14761 gtcggcgttt tgcttttaag tgataaaaac cgcagaaaca gacgttccct ccataaatct
    14821 cttgctattc cttttataaa aagttcgcag tacccatacc tggcccccag cctcgccgct
    14881 ggtagttcgt gtcatgtttt ttacgcgtaa aacacgacaa aagcatcaat acagcgatcg
    14941 tctcacttgt atgataataa aaacaatatg ttatat
//
LOCUS       DAZGJT010000106        14962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZGJT010000106 DAZGJT010000000
VERSION     DAZGJT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.106"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(18..171)
                     /gene="hokE"
                     /locus_tag="ACHKFK_003821"
                     /pseudo
     CDS             complement(18..171)
                     /gene="hokE"
                     /locus_tag="ACHKFK_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
     gene            616..1734
                     /locus_tag="ACHKFK_003822"
     CDS             616..1734
                     /locus_tag="ACHKFK_003822"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO9054065.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSTLIDVVKNQ
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRSLTEATLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGE"
     gene            1800..2048
                     /locus_tag="ACHKFK_003823"
     CDS             1800..2048
                     /locus_tag="ACHKFK_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO9054066.1"
                     /translation="MKHSLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTTFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFFLRFIWRRWFSLAD"
     gene            2113..2496
                     /locus_tag="ACHKFK_003824"
     CDS             2113..2496
                     /locus_tag="ACHKFK_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO9054067.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRLRPDSTVNQ"
     gene            2575..3228
                     /gene="nfsB"
                     /locus_tag="ACHKFK_003825"
     CDS             2575..3228
                     /gene="nfsB"
                     /locus_tag="ACHKFK_003825"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO9054068.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAADNYVFNERKMLDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDEEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            3336..4583
                     /gene="ybdG"
                     /locus_tag="ACHKFK_003826"
     CDS             3336..4583
                     /gene="ybdG"
                     /locus_tag="ACHKFK_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO9054069.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4740..6122)
                     /gene="pheP"
                     /locus_tag="ACHKFK_003827"
     CDS             complement(4740..6122)
                     /gene="pheP"
                     /locus_tag="ACHKFK_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO9054070.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRKVN"
     gene            complement(6224..9367)
                     /gene="cusA"
                     /locus_tag="ACHKFK_003828"
     CDS             complement(6224..9367)
                     /gene="cusA"
                     /locus_tag="ACHKFK_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO9054071.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQVGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDTMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVSEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(9379..10602)
                     /gene="cusB"
                     /locus_tag="ACHKFK_003829"
     CDS             complement(9379..10602)
                     /gene="cusB"
                     /locus_tag="ACHKFK_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO9054072.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKEEPPAEKTSTAERKVLF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFSQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGAPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(10618..10950)
                     /gene="cusF"
                     /locus_tag="ACHKFK_003830"
     CDS             complement(10618..10950)
                     /gene="cusF"
                     /locus_tag="ACHKFK_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO9054073.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KDVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10974..12356)
                     /gene="cusC"
                     /locus_tag="ACHKFK_003831"
     CDS             complement(10974..12356)
                     /gene="cusC"
                     /locus_tag="ACHKFK_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO9054074.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMAALKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTRELSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLPSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12513..13196
                     /gene="cusR"
                     /locus_tag="ACHKFK_003832"
     CDS             12513..13196
                     /gene="cusR"
                     /locus_tag="ACHKFK_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO9054075.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            13186..14628
                     /gene="cusS"
                     /locus_tag="ACHKFK_003833"
     CDS             13186..14628
                     /gene="cusS"
                     /locus_tag="ACHKFK_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO9054076.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLANSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQAGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLGDEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     TGETIVVRCQTVDHLVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIILPA"
     gene            14778..>14962
                     /locus_tag="ACHKFK_003834"
     CDS             14778..>14962
                     /locus_tag="ACHKFK_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054077.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYR"
BASE COUNT         3618 a         4064 c         3663 g         3617 t
ORIGIN      
        1 tactccccgc acaacggcta cttcttcggt tcgtaagcga gaacagcctt aaactcaata
       61 ttacgttcct ttacggtaaa ctcacacagc gagtctccga cctagaagcg taaatcccag
      121 caccgttaaa cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt
      181 gcctccatgc gaagaaggga ctaccatcca aattgttcag gtttgggtag gtagccccag
      241 ggttgataga aatatcgtcc tggggcttca tctttccaga cctcagagta aacctgaaac
      301 cggaaagctt caggcacccg cagttatctt acccttcctt agcaaaatag ctgtttattt
      361 atacagtgag tcctgtttcg gacttctcat ttaatcaact gaagctgaac ccgttttttc
      421 tgcatgcctc aatcgtcaaa attcttcgcc gccagccagc tctgccataa cttgtcacat
      481 tgcaaaagta gccaaattgc ttcagtcaca atcagttctc catcaccaaa aaaagaaaca
      541 ttccagaact tccgcatctc acccagccgc tcccacttct atactgaata gaaaacgcca
      601 acataagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt taccctcggt
      661 attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca ggactcttca
      721 acgctgattg acgtggttaa aaatcagatc acggccggag aggtaaagca cgatatcacc
      781 gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc tgccgggcaa
      841 ttttcagcga tgcagaaagt cgtattgcag gcagcggcag accatcatct ggaaatttgc
      901 ggcggtggca cgcatccgtt tcagaaatgg cagcgtcagg aggtatgcga taacgaacgc
      961 tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt ttttggtcag
     1021 catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca cggcttgtca
     1081 cggtttgtgc cgcactttat cgccctttcc gccgcgtcgc catatatgca gggaacggat
     1141 acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa tggcccaatg
     1201 ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgcctgag ttacaccacg
     1261 atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagccccca ttttggtacg
     1321 gtggaagttc gggtgatgga taccccgtta acccttagcc acgcggtaaa tatggcggga
     1381 ttaattcagg ctaccgccca ctggttactg acggaacgcc cgtttaaaca tcaggagaaa
     1441 gattacctgc tgtataaatt caaccgtttc caggcctgtc gctatgggct ggaaggcgtc
     1501 attaccgatc cgcatactgg cgatcgtcgg tcactaacgg aagccacctt gcgtttactg
     1561 gaaaaaatcg ccccttctgc acataaaatt ggcgcatcga gcgcaattga agcactgcac
     1621 cgtcaggtcg tcagtggtct gaatgaagcg cagctaatgc gtgatttcgt cgccgatggt
     1681 ggctcgctaa ttgggctggt gaaaaagcat tgtgagatct gggctggaga ataagtcggg
     1741 aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt aacaatgtta
     1801 tgaaacactc tttagaaacc ttgaccaccg cagcaggcat tttgctgatg gctttcctct
     1861 cttgcctgct gctgcccgcc cccgcactgg ggctaacgct ggcacaaaaa ctggtgacca
     1921 cgttccatct gatggatctt agccagcttt acaccttatt gttttgtctg tggtttttag
     1981 tgctgggcgc tattgagtat ttttttctac gctttatctg gcgacgctgg ttctcgctgg
     2041 cggattaaac gtcgattatc gccgaacttc gcggcatact ttgcttattc tccttcgcct
     2101 aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct ggctcttgag
     2161 ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt taaaattggc
     2221 ggcaagattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa tctgaagtcc
     2281 gatccacaaa aatccctgct aaatcagcaa atatacccaa gcatcaagcc agggtatcac
     2341 atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga agcgttactt
     2401 cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa acgcgatcaa
     2461 aaacgactgc gtcctgactc aaccgtaaat caatagcgca tttttctcgc ttaccatttc
     2521 atctcgatcc ttgtaatctg ctggcacgca aaattacttt cacatggagt ctttatggat
     2581 atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag caaaaaactt
     2641 accccggaac aggccgagca gatcaaaact ctactgcaat acagcccatc cagcaccaac
     2701 tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg tgttgccaaa
     2761 tccgctgccg ataattacgt gttcaacgag cgtaaaatgc ttgatgcctc gcacgtcgtg
     2821 gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt tgaccaggaa
     2881 gatgccgatg gccgctttgc cacgccggaa gcgaaagccg cgaacgataa aggtcgcaag
     2941 ttcttcgccg atatgcaccg taaagatctg catgatgatg cagagtggat ggcaaaacag
     3001 gtttatctca acgtcggtaa cttcctgctg ggcgtggcgg cgctgggtct ggacgcggtg
     3061 cccatcgaag gttttgacgc cgccatcctc gatgaagaat ttggtctgaa agagaaaggc
     3121 tacaccagtc tggtggttgt tccggtgggt catcacagcg ttgaagattt taacgctacg
     3181 ctgccgaaat ctcgtctgcc gcaaaacatt accttaaccg aagtgtaatt ctcccttgcc
     3241 gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc tgtttcagcc
     3301 gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata tcccaggttg
     3361 aagatttagc gggtattgag attgatcaca ccacctcgat ggtgatgatt ttcggtatta
     3421 tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg cggaccttcg
     3481 aaaaacgcgc catcgccagt tcacggctat ggttgcaaat cattacccag aataaactct
     3541 tccatcgttt agcttttacc ctgcagggga ttatcgtcaa tatccaggcg gtattctggc
     3601 tacaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg tggatcatga
     3661 tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg gcgcagaaat
     3721 tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa ctgatcggcg
     3781 cgattctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca gcgattctga
     3841 tcagcggtct tggtgcaatg gctgccgtgc taatgttggt atttaaagat ccgattcttg
     3901 gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc gactggctgg
     3961 agatgccgaa atacggcgcg gatggcgcgg tgatcgatat tgggttaacc accgtcaaag
     4021 tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt tctgactcct
     4081 ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc agtatcagta
     4141 ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg aataaagcgc
     4201 atttgttaaa gccttattta accagccgcc atcaggaaat taatgagtgg aatcgccagc
     4261 aaggttctac ggagtcggta ttaaacctgc gccgaatgac caatattgga acctttcgtg
     4321 cctatctgaa cgaatatctg cggaatcatc cgcggattcg caaagatatg accttaatgg
     4381 tacgccaact ggctcctggt gataacggtt taccgcttga gatctatgcg tttaccaaca
     4441 ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac atatttgcga
     4501 ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat attcgctctc
     4561 tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga tgagagcggt
     4621 ttcggatggt tgacatcgtt ttgtcggatg cggcgtgaac gccttatccg acctacagaa
     4681 tcgttacaaa ttgtaggccc gataagcttg cgcatcgggc aaatcagcct cacatcagct
     4741 tagttcactt tcctacgcaa tgttttaaac gccacaaaca ggaatacaat ccacaccggc
     4801 agcagaatcg ctgacaagcg catatcatcc atcgtgcaca tcagcagcaa aatcatgccg
     4861 aggaaggcga tgcaaagata gttgccgaac ggatagagca gcgccttaaa ttgtgtttca
     4921 cgcccctgac gtcgcatcgc cgcgcgaaaa cgcagatgcg ccaggcagat cataatccag
     4981 ttcaacagca gcgttgctac caccagcgcc atcagcagac caaacgcttt ttgcggcagc
     5041 agatagttga ttaacaccac cagcgaagtg atcgctccgg aaagcatcag cgagttaatc
     5101 ggcacgccgc gacggctgac gcgagtcaaa aactttggcg cattaccctg cacagaaagg
     5161 ccaaacagca tgcggctgtt agagtaaacc ccgctgttat acaccgacag cgatgccacc
     5221 agaatgacga agttcagcgc agaagccacc acattgctgt cgagattatg gaaaatcatc
     5281 acaaacgggc tactgttgga tttcacttcc acccacgggt agagcgccag taaaaccacc
     5341 agtgaaccga tataaaacag caggatgcga tacaccacct gattcactgc tttagggatg
     5401 cttttttccg gatcgcgcgc ttcagccgca gtaatcccaa tcagctccag accgccgaag
     5461 gagaacataa ttaccgccag cgacaaaatc agtccattcc agccggtggc aaagaaaccg
     5521 ccgtagcgcc agaggttatc gatactggct ttctcaccgc cgtgcccaga aaacagcagc
     5581 cacaggccaa agccgatcat accgatgatc gccagcactt taatcagcgc aaaccagaac
     5641 tcggtttcgc catataagcg cacgttcacc aggttaacgg cgttgatgat aataaagaag
     5701 gcggcagccc aaatccacgt tggaacatcc gggaaccagt actgcatata gatgcccgca
     5761 gcggtcagct ctgccattcc caccagcacg aacattaccc agtagttcca gccagagagg
     5821 aagcccgcaa acggtcccca gtatttatag gcaaagtggg caaatgaacc ggataccggc
     5881 tcttcgacca ccatctcgcc gagctggcgc ataatcagga aagcgatgat cccggcgacg
     5941 ccgtagccca gcaatacagc cggacccgcc atctgaatcg ccgggccaat gccaagaaac
     6001 agaccagtac caattgcgcc acccagcgca atcagttgaa tatgacggtt atgtaatccg
     6061 cgatgaagcg tcggctcttg attcgacgca gtatcttccg atacggttga cgcgtttttc
     6121 acgcctttcc cctgtgtgtc ttttttgttg aggggcacct tttaacattt agtgcccatc
     6181 gtcgcaagac acaatccaca cggttaaacg gggtatcctg cttttatttc cgaacccgat
     6241 gtcggtgcag ccacatcagc ttatacgccg ccgggataat aaacagcgat agcaaaggcg
     6301 cggtgatcat gccgccaatc atcggcgcgg caatccggct catcacctct gaacctgctc
     6361 ccgttcccca cagaatcggc agcagaccag cgataatcac cgccaccgtc atcgctttcg
     6421 ggcgcacgcg caggaccgcg ccgtgataca acgcctcatc cagcttctgc tcgctgaatg
     6481 tttgcggatt attcaacgac ggctcggcct ctatggcgtg acgtaaatac atcagcatca
     6541 ccacgccaaa ttcggcggcg accccggcaa gggcaataaa accggtgccc gtcgccacgg
     6601 aaagatgaaa gcccatccac cacagcaacc agatgccgcc caccagcgca aacggtacgc
     6661 tgctgataat cagcaacgct tcgctcaccc gacggaacgc cagatacaac agcacgaaga
     6721 tgatcatcag cgtcatcggc accatcagtt tcagcttatg gttggcgcgc tccagtagct
     6781 cgaactgccc ggagaatgcc acgctggtgc ccggttttaa ctgcactttt tcagctatcg
     6841 ctttttgcag atcgtgaacc accgacacca tgtcacgatc gcgggcatcg atataaatcc
     6901 agctcgtcgg gcgcgcattc tcggttttca gcatcgacgg tccggtagag actttaacgt
     6961 cggccacgtc cgccagagtg atttgctgct tcatcggcgt gaggatcggc aactggcgca
     7021 gtgcctgcgg gctatcgcgc cagctttgcg gataacgcag attaattgga taacgagcaa
     7081 tcccttccac cgtttcgcca accatcgccc cgcccaccgc agaagtcaca aacaactgca
     7141 catccgccac cgtcataccg taacgcgcgg ctttttcacg gttaatctca acgttgatat
     7201 agcgcccacc ttccagacgc tcagcaagcg cagaagccac gcctggcacc gttcgcgcca
     7261 cttcttcaat ttgctccgcc atcgtgtcga tatccgccag cacagtgccg gaaactttaa
     7321 tgccgatagg gcttttaatg ccagtcgaga gcatatcgat acggttacga attggcggca
     7381 cccacagatt cgccagcccc ggcagacgca cggtgttatc cagttcctca atgattttgt
     7441 ccatcgtcat gccaggccgc cactgatcct gcggcttaag ctggatagtc gtttctacca
     7501 tttccagcgg agcagaatcg gtggcggttt ccgctttccc ggttttgcca aatacccgcg
     7561 ccacttcagg tacgctcata atcagcttgt cggttttttg cagcatactc gccgcctccg
     7621 ctgcggaaat ccccggcagc gtcgatggca tatacaacaa gtcgccttca ttgatctgcg
     7681 gtaaaaattc cccgccaact ttattgagcg gccagagaac cgtcagcacc gaaagcgctg
     7741 ccaccaacag cgtggttttc ggccagtgca gtactttcag caacagcgga tgataaacac
     7801 gaatcaaaaa gcgattgagc gggttactgc tttctggcgg aattttgcca cggatccagt
     7861 agcccatcag gatcgggatc actacaatcg ccagtagcgc cgcgcccgcc atcgcatagg
     7921 ttttggtgaa cgccagcggg ccaaacagac gaccttcctg cccttccagg gtgaagatcg
     7981 ggataaacga cagcgtgata atcagcagac tgataaacag cgccggcccc acttcaacag
     8041 atgcatcggt gatcacctgc cagcgcgttt tattatccag cgtggcgtca ggatgctggt
     8101 gctgccactc ttccagccgt ttatgcgcat tctcgatcat gacgatagcg gcatcgacca
     8161 tcgccccgac ggcaatcgca atgccaccca acgacataat attggcgttc agtccctgaa
     8221 agtgcatgac aataaaagca atacacaacc ccagcggcaa cgaaataatc gccaccagcg
     8281 ccgagcgcac atgccagaga aacagcgcac agactaccgc caccacaata aactcttcca
     8341 gcaacttgcc gctgaggttg tcaatggcgc ggtcaatgag ctggctgcga tcgtatgttg
     8401 taactatctc cacgccttcc ggcagactac ttttcagcgt ttccagtttg tccttcacgg
     8461 cggcgatcac ttctcgggcg tttttgccgg aacgcaggat caccaccccg cccgccactt
     8521 cgccttcgcc gttcagttcg gcaatgcccc ggcgcatctc cgggccaacc tggaccttcg
     8581 caacatcacg cagataaacg ggcacgccat tttcactggc ttttaaaacg atgtgattaa
     8641 agtcgtcgag cgtttgcaga tagccgctgg cgcgcaccat atattccgct tccgccagtt
     8701 cgatcgacga accgcccgct tcctggtttg aagcatccag cgcgcttttt acttcggcga
     8761 gactgatgcc atactgcgcc aggcgctgag gatcgataac cacctgatac tctttcacca
     8821 caccgcccac cgacgccact tccgcaacgt cagggatggt ttttagctca tatttgagaa
     8881 accagtcctg caatgagcgt aaatcggcca gatcgtgctt accgctgcga tccaccagtg
     8941 catattcata gatccagcca acacccgtgg catctggccc cagctcggcg ctgactcccg
     9001 caggcagctt accctgtacc tggttgaggt actccagcac ccgcgagcgc gcccagtacg
     9061 gatcggtgcc atcttcgaaa atgacataca cataagagtc gccaaactgc gagaaaccgc
     9121 gcacagtctt cgcgccaggc accgacaaca tggtggtggt tagcggataa gtcacctgat
     9181 tttcaacgat ttgcggtgcc tgaccgggat agctggtttt aataatcacc tgcacatcgg
     9241 aaagatccgg cagtgcatcc actggcgtgt taatgatagt ccaggtgccc cagatactca
     9301 gaaacaacgc gcccatcagc accagaaaac ggttcgccac cgagcgacga ataatccatt
     9361 caatcattgg ctattccctc aatgcgcatg ggtagcactt tcagagcgca tccgctccag
     9421 tgcgccagaa atattggctt cagaatcaat caggaacagg ccgctggaaa ccaccttttc
     9481 accttccgcc agaccagagc gtaacgcggt gacgccttgc gatgcctgga aaacggcaac
     9541 gcgtttcggt acaaagcgcc cgtcagcatc aacggtaatc acccgctgtt cgttaccggt
     9601 atcaatcagc gcctgtgacg gaatgagcag catcggttcg ctggcggtat tgagttgcaa
     9661 ccaggcgttc atgcccggtt ttagcgcctc gtcggcgttg tcgacttcca gacgcagttg
     9721 cagcgtgcgg gtcgcagcat ccacgccagg tagcagcgtc catttgcgga tggtgagtgt
     9781 tttatcaggt cgcgccggaa cggtgagcgt aaactgcgag gcatctttca ccagccaggc
     9841 gatggactcc gggatcgcag cagtgaccca caccgggtcc ataccctgaa ttttcgcgac
     9901 cacgttatct ttggcgatat tcattcccgc gcgcagatca aacgcggtga tcacgccatc
     9961 aatgggcgct ttgagcgtaa agcgagtctg gattttttgc gtggcgatca ggcggcgaat
    10021 atcagcctcc ggcattcccg ccagtcgcag tcgctcaaga atgccttcag tctgggtcgc
    10081 cgtaccgccg gtttcgcgca gcagtaaata ctcactctgc gcttccaccc agtcgggaat
    10141 ggtcaggtcg agaagcggcg cgcccttttg cactttatcg cccacggtaa gcggatacac
    10201 cttgtcgata aacccggcgg cgcgggcctg cacaatggca tactgatact cgttgtaact
    10261 gacattcgcc gggaaactct gggaaaaagt cagcggcccg cgcgtgacgg tcgccgtttt
    10321 cacccccaga ttctgcgtct gagtcgggtc aatgcgcaca ccagacgcag aactctcttc
    10381 atcggcatat ttcggcacca gatccatatc cataaacggc gatttccctg gtttatcgaa
    10441 ccgcgtattg ggatacatcg ggtcgtacca gaataagact ttacgttctg cggtcgacgt
    10501 tttttctgcg ggcggttcct cctttgcaaa ccaggtaaaa cctgccgcag aaataatacc
    10561 gcccgcgatc atgctgccga taataagcgc gatttttttc atctcattaa acccggatta
    10621 ctggctgact ttaatatcct gtaataaaga aaggttgccc tgttggacaa aattaaacgc
    10681 tactttatcg ccggttttaa tttcgctcat tttcgtctgc ggggtgatgg taaagcgcat
    10741 agtcatctcc ggccagttca cggcagcaat cggatcgtga tggatggtga tttttttgct
    10801 ttccagatca acatctttta ccacgccagt ggcgctaata acctgtggtt gtgcttcgct
    10861 catggtttca tgatgatgtt cgttagcctg ggcattaaag ccaataacgg taaacagact
    10921 aaacattgcg acttgcagtg cttttttcat ttattctctc ctggagttaa aaattattgc
    10981 tgccaaccgc cccctaaggc ggtatataaa gaaatttcgt taacctgacg ggcataattc
    11041 aggtcaagta aagtttgtcg ggttgcaaat aaagaacgct cggcatccag cacttccaga
    11101 taacttaccg cgccgtgctg atataatgcc cgcgcccgtt gcaaagtaat ttgcagcgac
    11161 gcaagataac gctgctgggc gctgatttga tcgttcaggc tttgacgtaa tgccagcgca
    11221 tctgccactt ctttaaaggc gttctgaatt ttctgttcat aattcaccac cgactgctgc
    11281 tggcgaattt cggcgatatc cagattggcc tggttgcgtc cagcattaaa aatggggatc
    11341 tcaattttag gaataaaatt ccacatcccg ctgctggcat taaataatga cgacagatcg
    11401 ctgctggcgg ttgatattcc gctggtcagg ctgatagacg ggaaaaatgc cgcacgcgca
    11461 gcaccaatat tggcattagc cgccattaac gcgtgttcag cttccataat atcagggcgc
    11521 tgcaataaga tttgcgacgg caagcccgcc ggtaatttaa cgctttgcag gctgtcgctg
    11581 tttactgtct gcgcttgcgg cagcttgccg tagcttccca ataacagttg caatgcatta
    11641 tttgcctgcg ccagttcccc ctgacgttta gcgatgtcgc tgcgggtact ttctatcacc
    11701 ccgcgagcct gttccagcgc cagaacattg ctgctaccgg tcaacagttg tttttcgaca
    11761 aacgcatatg actgctgata attacgcagc gtttcttcgg ctatttgcaa ttgcgcatac
    11821 gccagttgct gattgaaata gctttgcgcg acattagaaa ccaacagaat atgcaccgcg
    11881 cgctgagctt cctcagtggc taaataattt tggcgctcgg cttcgctcat gttctttaag
    11941 cgaccgaaaa aatcgagatc aaagctggcg ttaaggccag tcgataactc ccgcgtcgtg
    12001 gctgaatcgc ctttaagatt gccgctccag ctgccgctgc cctcgccatt gagctgtggg
    12061 tagcggtcgg catcggtcag acgatattgc gcccgcgctt cctgcacttt cagcgccgcc
    12121 atgcgcaaat cccggttatt caccaacgcc tcgctaatca gcgtcttcac ctgattatca
    12181 acaaaaaagg tgcgccagcc cgcgttctgg tagttatctg ctgcgttaac caggccgttc
    12241 tggctgagtg agaactgctg cggcacgggc attgccggac gctgataatc cggtgccagt
    12301 gaacaaccgg ttagcgcaag ggccacacaa aatggcagaa gtttacaagg agacataggc
    12361 tcataatttc tggtgatttt atgctgccaa ctttactcgc caggctctga ttttccggtg
    12421 acaggaaaat gacaaaattg tcattttgcc aataagcgat tgccatctga tcccgctact
    12481 ctagaattgc ccgggcaaca tgcggaggaa atatgaaact gttgattgtc gaagatgaaa
    12541 agaaaaccgg agaatacttg accaaagggt taaccgaagc cggttttgtg gtcgatttgg
    12601 ccgacaacgg gctgaatggc taccatctgg cgatgaccgg tgattatgat ctgataatcc
    12661 tcgatattat gctgccggac gtgaacggct gggatatcgt gcgcatgttg cgttccgcca
    12721 ataaggggat gccgattctg ttgcttaccg cgcttggcac cattgaacat cgcgtcaagg
    12781 ggctggagtt gggggcggat gactatctgg tgaagccgtt cgcttttgct gaactgctgg
    12841 cgcgggtgcg cactctactg cggcgcggag cggcggtaat tatcgaaagt cagtttcagg
    12901 ttgccgatct gatggtcgat ctcgtcagcc gcaaagtcac ccgcagcggc acgcgcatca
    12961 ctctgaccag taaagaattt actctgctgg agtttttcct ccgccatcag ggcgaagtgc
    13021 tgccccgctc gcttatcgcc tcgcaggtat gggacatgaa ttttgacagc gataccaacg
    13081 ccattgatgt ggcggtgaag cggctgcgcg gcaaaatcga caacgacttt gagccaaagt
    13141 tgattcagac cgtgcgcggc gtgggttaca tgctagaggt gccggatggt cagtaagcca
    13201 tttcagcgcc cgttttcgct ggcaacccgc ctgacctttt ttatcagcct cgccaccatc
    13261 gcggcgtttt tcgcctttgc atggatcatg atccactcgg taaaagtgca ttttgccgag
    13321 caggatatta atgatttaaa ggagattagc gccacccttg aacgggtact taatcaccct
    13381 gacgaaacgc aagcccgacg cttaatgacg ctggaagata tcgtcagtgg ttattccaat
    13441 gtgttgatct ccctggcaaa tagccacggt aaaacggtgt atcactcccc cggtgcgccg
    13501 gatatccgcg agtttacgcg tgacgccata cccgataaag acgcgcaggc tggcgaggtg
    13561 tatctccttt ccggcccgac gatgatgatg ccagggcacg gtcacgggca tatggaacac
    13621 agcaactggc ggatgattaa cttgccggtt ggcccgctgg ttgacggcaa accgatttat
    13681 acgctctaca tcgcgctttc gatcgatttt catcttcatt acatcaatga tttgatgaat
    13741 aaacttatta tgaccgcatc ggtaatcagc atcctgatcg tctttatcgt gctgttggcg
    13801 gtacataaag gtcacgcgcc gatccgcagc gtcagccgtc aaatccagaa tatcacctcg
    13861 aaagatctcg acgttcgcct tgacccgcag accgtgccca ttgagctgga acagctggta
    13921 ctgtcgttca accatatgat cgagcgtatt gaggatgtct ttacccgcca gtccaatttc
    13981 tcagcggata tcgcccacga aattcgcaca cctattacta atctcattac gcaaacggaa
    14041 atcgccctca gccagtcgcg cagccagaag gagctggaag atgtgctcta ctctaatctc
    14101 gaagagctga cgcgaatggc gaaaatggtc agcgatatgc tgtttctcgc tcaggccgat
    14161 aacaaccagc taatccccga aaagaaaatg ctcaacctgg gggatgaagt cggcaaagtg
    14221 ttcgattttt tcgaggcgtt agcagaagat cgcggcgtgg agctgcggtt tgttggcgac
    14281 gagtgtcagg ttgcgggcga tccgctgatg ctgcgtcggg cgttaagtaa cctgctttct
    14341 aatgccctgc gttatacgcc aaccggagag acaattgttg tgcgctgcca gacggtcgat
    14401 cacctggtgc aagttaccgt cgaaaacccc ggtacgccca ttgcgcccga acacttaccg
    14461 cgattgtttg accgtttcta ccgcgttgat ccttcccgcc agcgaaaagg cgaaggtagc
    14521 ggcattggtc tggcgatagt gaaatcgatt gttgtcgcgc ataaaggcac tgttgcagta
    14581 acgtcagatg cacgggggac aaggtttgtt atcatattac ccgcttaatc cttcagcaaa
    14641 cggcaacttt tataaccagt gtaaaaataa catgccgcaa tagtaatacc tattaatgat
    14701 aaaaagctgt cacaattcat aaaaaacctt aatatacgcc accctaaaca taaccagcgt
    14761 taatgtaagg tttttgtgtg gactggcttc ttgatgtttt tgctacctgg ctctacggct
    14821 taaaagtaat cgcgataacg ttagcggtca ttatgttcat cagcgggctg gacgattttt
    14881 ttattgatgt cgtctactgg gtacgccgca ttaaacgcaa gttgagtgtt tatcgccgct
    14941 acccgcgaat gagttaccgc ga
//
LOCUS       DAZGJT010000107        14907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZGJT010000107 DAZGJT010000000
VERSION     DAZGJT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.107"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            10..834
                     /gene="gadX"
                     /locus_tag="ACHKFK_003835"
     CDS             10..834
                     /gene="gadX"
                     /locus_tag="ACHKFK_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO9054078.1"
                     /translation="MQPLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIQV
                     DKCVENFVLVSRDTLSLFLPMLKEEALKLHVHKKVPSLLVHHCTRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDTQFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEGTSYSQLLTECRMQRALQLIVIYGVSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQELPNCGSAASMAAQGIFYGTDRSAEGIRL"
     gene            1203..1931
                     /gene="gadW"
                     /locus_tag="ACHKFK_003836"
     CDS             1203..1931
                     /gene="gadW"
                     /locus_tag="ACHKFK_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO9054079.1"
                     /translation="MAHVCSVILVRRSFDIHHEQQKISLHNESILLLDKNLADDFAFC
                     SLDTRRLDIEELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPIQLVTAIFDSFNDPR
                     VNSPILSKMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFSQHSPGTFS"
     gene            complement(2294..5407)
                     /gene="mdtF"
                     /locus_tag="ACHKFK_003837"
     CDS             complement(2294..5407)
                     /gene="mdtF"
                     /locus_tag="ACHKFK_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO9054080.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     ISATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIK
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGAFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKNNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPNQVIGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPVEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5432..6541)
                     /gene="mdtE"
                     /locus_tag="ACHKFK_003838"
     CDS             complement(5432..6541)
                     /gene="mdtE"
                     /locus_tag="ACHKFK_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO9054081.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAEAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYGQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6649..6978
                     /locus_tag="ACHKFK_003839"
     CDS             6649..6978
                     /locus_tag="ACHKFK_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054082.1"
                     /translation="MRDLQTSGIVGLSASKMGCKHSDRHGEVNDKKNVAVLPTVSASI
                     RATKGSTGGYTYQGNKVVFVSPSIITGGIEISICIPRHASHQFQLLMSLLKIRCDTQG
                     DDVAHTS"
     gene            complement(6928..7455)
                     /gene="gadE"
                     /locus_tag="ACHKFK_003840"
     CDS             complement(6928..7455)
                     /gene="gadE"
                     /locus_tag="ACHKFK_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO9054083.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8254..8826)
                     /gene="hdeD"
                     /locus_tag="ACHKFK_003841"
     CDS             complement(8254..8826)
                     /gene="hdeD"
                     /locus_tag="ACHKFK_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO9054084.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKKQ"
     gene            9081..9413
                     /gene="hdeA"
                     /locus_tag="ACHKFK_003842"
     CDS             9081..9413
                     /gene="hdeA"
                     /locus_tag="ACHKFK_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO9054085.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKKASFKDKVKGE
                     WDKIKKDM"
     gene            9529..9855
                     /gene="hdeB"
                     /locus_tag="ACHKFK_003843"
     CDS             9529..9855
                     /gene="hdeB"
                     /locus_tag="ACHKFK_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO9054086.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            9919..10566
                     /gene="yhiD"
                     /locus_tag="ACHKFK_003844"
     CDS             9919..10566
                     /gene="yhiD"
                     /locus_tag="ACHKFK_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO9054087.1"
                     /translation="MTAEFIIRLILAVIVCGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLGHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10618..11388)
                     /locus_tag="ACHKFK_003845"
     CDS             complement(10618..11388)
                     /locus_tag="ACHKFK_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HFO9054088.1"
                     /translation="MISAQNLVYSLQGRRLTDNVSLTFPGGEIVAILGPNGAGKSTLL
                     RQLTGYLQPDSGECRLFNKPLNEWSITELAKHRAVMRQNSHMAFPFSVQEVIQMGRHP
                     HRTGNQDNETAQIMALCDCQALANRDYRQLSGGEQQRVQLARLLVQLWEPTPSPKWLF
                     LDEPTSALDIHHQQHLFRLLRQLVHERQFNVCCVLHDLNLAARYADRVVLMQKGKVIA
                     NGKPQDVLTQQALTMLYGADITVLKDPANHSPLIVLDH"
     gene            complement(11385..12377)
                     /gene="shuU"
                     /locus_tag="ACHKFK_003846"
                     /gene_synonym="chuU"
     CDS             complement(11385..12377)
                     /gene="shuU"
                     /locus_tag="ACHKFK_003846"
                     /gene_synonym="chuU"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000910402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease ShuU/ChuU"
                     /protein_id="HFO9054089.1"
                     /translation="MLKDSLSSARVFMGLSLLLLALVLFGASQGALKISFDALFDEEY
                     RDIWLNIRLPRVLLAVLVGAALATAGVIMQGLFRNPMADPGLLGVSSGSALMVGVAIV
                     LPFSFPVVLVLYEQMVFAIAGSLVVCTIIFLITQRHRDGSMMQLLLAGIAINALCGAA
                     IGILSYIGDEQQLRQLTLWMMGNLGQAQWPTLLVASSFILPAIIATTCLAGTLNLLQL
                     GDEEAHYLGVNVKRKRQQLLLVSSLLVGAAVSVSGIIGFIGLVIPHLIRMTTGANHRW
                     LIPCSALAGACLLLMADTLARTLVQPAEMPVGLLTSLLGGPYFMWLILRNRRIT"
     gene            complement(12426..13049)
                     /gene="chuY"
                     /locus_tag="ACHKFK_003847"
     CDS             complement(12426..13049)
                     /gene="chuY"
                     /locus_tag="ACHKFK_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobilin reductase"
                     /protein_id="HFO9054090.1"
                     /translation="MTPWLLFGAGGKGVGARTLELALAEQRPVVAVIRHADAATKLAQ
                     QGVQVFTGDACDASVVAAACRAAGPDALIISTMGGAQDYLAHRTVIDEAEKAGISRMI
                     LVTSLGCGDSWPFLSERAKAAFGQAVREKTLAESWLQTSQLDYAILRPGGLLDGAATG
                     KAQRIQNQECHGFVNRADVAAHIHELANAPALNQQVYSLIEPDLKPA"
     gene            complement(13049..13543)
                     /gene="hutX"
                     /locus_tag="ACHKFK_003848"
     CDS             complement(13049..13543)
                     /gene="hutX"
                     /locus_tag="ACHKFK_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme utilization cystosolic carrier protein
                     HutX"
                     /protein_id="HFO9054091.1"
                     /translation="MSHVSLQEFLKTEPDGTLEAIAEQYNTTLLEVVKNLPSPTVVSG
                     DKFDTVWDTVCEWGNVTTLVHTTDVILEFSGELPSGFHRHGYFNLRGKHGMSGHIKAE
                     NCTHIALIERKFMGMDTASILFFNKEGSAMLKIFLGRDDHRQLLSEQVSAFHALAVSL
                     KEHA"
     gene            complement(13556..14893)
                     /gene="hutW"
                     /locus_tag="ACHKFK_003849"
     CDS             complement(13556..14893)
                     /gene="hutW"
                     /locus_tag="ACHKFK_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme anaerobic degradation radical SAM
                     methyltransferase ChuW/HutW"
                     /protein_id="HFO9054092.1"
                     /translation="MNTNNTLDLTPHFALDGDQPFKDRRAMMPFRGAIPVAKEQLAQT
                     WQEMINQTVSPRKRLVYLHIPFCATHCTFCGFYQNRFNDDACAHYTDALIREIEMEAD
                     SVLHQSAPIHAVYFGGGTPSALSAHDLARIITTLREKLPLAPDCEITIEGRVLNFDAE
                     RIDACLDAGANRFSIGIQSFNSKIRKKMARTSDGPTAIAFMESLVKRDRAAMVCDLLF
                     GLPGQDAQTWGEDLAIARDIGLDGVDLYALNVLPNTPLGKAVENGRTTVPSPAERRDL
                     YLQGCDFMDDAGWRCISNSHWGRTTRERNLYNLLIKQGADCLAFGSGAGGSINGYSWM
                     NERNLQTWHESVAAGKKPLMMIMRNAERNAQWRHTLQSGIETARVPLDELTPHAEKLA
                     PLLAQWHQKGLSRDASTCLRLTNEGRFWASNILQSLNELIQVLNAPAIALEKP"
BASE COUNT         3949 a         3524 c         3559 g         3875 t
ORIGIN      
        1 aagtcaacta tgcaaccact acatggtaat tgtctaattg cgtacgcaag acataaatat
       61 attctcacca tggttaatgg tgaatatcgc tattttaatg gcggtgatct ggtttttgcg
      121 gatgcaagcc aaattcaagt ggataagtgt gttgagaatt tcgtattagt ttcaagagac
      181 acgctttcat tatttctccc gatgctcaag gaggaggcat taaaacttca tgtacataaa
      241 aaagttcctt cattactcgt tcatcactgt accagagata ttcctgtttt tcaggaagtt
      301 gcgcaactat cgcagaataa gaatcttcgc tatgcagaaa tgctacgtaa aagggcatta
      361 atctttgcgt tgttatctgt ttttcttgag gatacgcagt ttataccgtt acttctgaac
      421 gtgttgcaac cgaatatgcg aacacgagtt tgtacagtca tcaataataa tatcgcccat
      481 gagtggacat tagcccgaat cgctagcgaa ttgttgatga gtccaagcct gttaaagaaa
      541 aaattgcgcg aagaaggtac gtcatattca cagttgctta ctgagtgtag gatgcagcgt
      601 gctttacaac ttattgttat atatggggtt tcaattaagc gagttgcagt atcctgtgga
      661 tatcacagcg tgtcgtattt catttacgtt tttcgaaatt attatgggat gacgcccaca
      721 gagtatcagg agcgatcggc gcaggaatta ccgaattgtg gctcggcggc aagtatggct
      781 gcgcaaggga ttttttacgg cactgaccgt tctgcggaag gaataagatt atagagtttt
      841 actcagacat aaaaaaaacc cggcataggg gaccgggaag aggatagtct gccgtctcca
      901 gactaataaa ccgttataac actccctgtt ggcacgggaa actttgtgct ctcagtaagt
      961 taaatataac ttttactgga aataagatca gccatttttt tataaacata agctatacgc
     1021 tgtgtgaaaa tataatcgtg ttgcatttac ttattatcaa ttaactgtta tgcaaaacta
     1081 ctttgtggat aaaatttggt cctaccaaat atggcagttt ttgcacacag aaacagtctg
     1141 gcgtcatttc attagtatac tgacattgaa ataatcgcag taatgaaata taagggatag
     1201 tcatggctca tgtctgctcg gtgatcctcg ttcgtcgttc attcgatatt catcatgaac
     1261 agcaaaaaat atcgttgcat aacgagagta tcctgctgct ggataaaaat ttggcagacg
     1321 attttgcgtt ttgttcactg gatacgcgac ggctggatat cgaagagctg acagtttgcc
     1381 attacttaca aaatattcgt cagttgccac gcaatttagg attgcatagc aaagatcgtt
     1441 tgttaattaa ccagtcaccc cccatacagc tggtgacggc gatttttgat agtttcaatg
     1501 acccccgggt caattcgccg atactgagca aaatgctcta tctttcctgt ttatcaatgt
     1561 tttctcataa gaaagaactg atccccttac ttttcaatag tatcagtact gtttcaggaa
     1621 aagttgaacg ccttattagc tttgatatcg ctaaacgttg gtatctacgc gatatcgcag
     1681 aaagaatgta caccagcgag agtctcatca aaaaaaagtt gcaggatgaa aatacctgtt
     1741 tcagtaaaat attactcgcc tccaggatgt cgatggccag acgattactc gagttacgtc
     1801 aaatacctct gcatactatt gcggaaaaat gtggctatag cagtacgtca tactttataa
     1861 atacatttag acaatattat ggtgtaacgc cacatcagtt ttcgcaacat tcgccgggta
     1921 ccttttcctg acatatttcg catttgaata taggtcagga tctcacgtct gcttcatgtg
     1981 aaactctttc ctgatgattt ctgccgggct accggctagt tctctttcgc agtttgccag
     2041 gcggtatagg tattttcagg aggaaaagac tcggcatgtt tgggattatt aagctaacaa
     2101 ttcatacgat tacgggaatg tgggtcagta ttgtgctgtt caaattgatg acgaatggct
     2161 ggagtggttt ctactttcag tgctgtgtac taagcctggt attcctgacg gttagctggt
     2221 tgttgagtgg tgaatggtta gcaggaaaga gtaaggctga accctcacgt tcaaccttac
     2281 tctcatttac acgttacgct tttttaaagc gggcaaagag atgttccact acaacgaaaa
     2341 agaccggaac gaagtaaatt gccagcactg ttgcggcaaa catcccgccc attacgccgg
     2401 tacctaccgc gttttgcgca ccagagccgg caccatgact gataaccagc ggcagcacgc
     2461 cgagaataaa ggccagagaa gtcatcagga ttgggcgtaa acgcatacgc gctgcttcga
     2521 tgattgcctc taccggcgtt ttcccttctt tctgcatcat ctcaacggca aattcgacaa
     2581 tcaggatggc gttcttggcg gaaagcccga tggtggtcag caaaccaacc tggaagtaaa
     2641 cgtcattact taagccgcgt agatcggtgg ccagtaatgc gccaacgacg cctaacggaa
     2701 caaccaacat caccgagaac ggaattgacc agctttcata aagtgcggcg agggcgagga
     2761 acaccacgac cagtgaaatg gcatatagcg cgggagcctg gtttgaggat aacgcttcct
     2821 gatacgacag tccggtccat gagtagccga cgcctgccgg aagttttgcg accaggtctg
     2881 ccataaattt catggcgtca ccggtacttt tcccggcagc cgcttcacct aaaatctcca
     2941 ttgacgggat gccgttgtag cgttccagtc gcggtgaacc ataggtccat tcggtagacg
     3001 aataggcaga aagcggtgcc atcgtgccag aggcgttgcg tacataccat tggttgatgt
     3061 tatccggcaa catacggaac ggcgtgcctg cctggacata cacttttttc acccgcccct
     3121 ggttgaggaa gtcgttcacg tagctgctgc cgaaggcggt ggaaattgtc tggttgatat
     3181 cagacagcgc cacacccata gcctcggctt tcgcggcgtt gacgttcact ttgaacatcg
     3241 gcgtatcttc caggccgttc gggcgtaccc cgatgacctg attcggtgat tgcgctgcca
     3301 gtgataacag ctcgtttcgc gcctgggtca gtttttcgtg ccccagattg ccgttgtcca
     3361 gcagttccat atcaaaacct gacgcggtac ccagttcagc caccgcgggt aagttgaacg
     3421 ggaagacgac ggctttattg atactgctta gcgcaatcat tgcccgctga ataatcgcgg
     3481 taaccgagtt ttcctcgccg acacgttcag accacggctt gaggctgata aacgccagac
     3541 cgttgttttg cccctgacca ctgaagccaa agccaccaac ggtaaacacc gactgaacat
     3601 tatttttctc ttttgtcaga taataatccg tcacctgttg tagcactttc gtggtgttaa
     3661 ccatggttgc accggaaggt aactgcgcgg tggtcataaa tgccccctga tcctcttctg
     3721 gtaagaaaga ggtcggcgta cgcaggaaca gcaccgccat cccggcgcaa atcagcaggt
     3781 agaccaccat gtagcgaccg gtacaacgca acagcgagcg ggtgctgtcg gtatagtgct
     3841 gagttgattt ttcaaacagc gtgttgaagc gtgcgaacag ggcgttaggt ttgtgaccgc
     3901 cctccggcgc ggctttcaga atggtggcgc acagggcagg ggtcaggctc attgctacaa
     3961 atactgaaag cagcatggag gagatcagcg tgatggagaa ctggcggtag atctccccgg
     4021 tcgcaccgct cataaaggcc atcggcataa acactgcgga aagaacaacg gcaatcccga
     4081 ccagcgcacg ttggatctgc cccatcgatt tatgcgtcgc ttccttcggc ggtagcttat
     4141 cttccgcaat gacacgctcg acgttctcca ccaccacgat ggcgtcatcc accagtaacc
     4201 ctatcgccag caccatcccg aacatagtta acgtgttgat ggtgaaaccg accgccgaca
     4261 agatcgcaaa cgtcccgaga ataaccaccg gtacggcaat cgtcgggatg attgtggcgc
     4321 ggaaattctg caaaaacaga tacatgacca ggaagacgag gatgatagcc tcaaccagcg
     4381 ttttgaaaac ttcctgaata gaaattttga taaacggtgt ggtgtcgtaa ggataaaccg
     4441 tcttcaggct tgcagggaaa taagccgata aacggttcag ctcctctttc accgctcgcg
     4501 aggtatccag ggcgtttgct ccggtagcca gtttgatggc gatcccggca gcaggtttgc
     4561 cgttatagcg cgccacggtg gaataatctt ccgccccaag ttcgacgcgg gcgacatcac
     4621 gcagcagcac ttgcgaacca tcttgctgaa ctttcaacag gattttgcca aattcttccg
     4681 gcgtttgcag acgcgtctgc acaatgatcg aggcgttcag ttgctggtct gccgcctgtg
     4741 gcatgccacc cagttgacca ccggaaatct ggttgttttg caccttaatc tgggaaataa
     4801 catcggaagg taccaggtta tatttattga gtttttgcgg gtccagccag atacgcatgg
     4861 cgtactcgga accaaagagc tgtacgctac caacgcccgc ggtacggctt agcgggtctt
     4921 tgatattaga cgctacatag tccgcgatat cgtactggtt gaggctgccg ttatcagaaa
     4981 taaatgccgc taccatcagg atattactgc tcgacttatc gacgctaatt ccctgctgct
     5041 gcaccgcttc aggtaatgaa ggcatagcga gttgcagttt attttgcacc tgaacctgtg
     5101 cgatatcagg tgatgtcccc gtctcgaagg tcagagtgat ggaggcatta cctgccgcat
     5161 cgctggttga agacatgtac atcaggccat caagcccatt catattttgc tcaatcacct
     5221 gagtgaccga gtcttctacc gtttgcgcat cggcacccgg ataggtagcg ctgatggtaa
     5281 tggtcggtgg cgcaatctgc ggatactgcg caaccggtaa gttcatgatc gccagaccgc
     5341 ctgcaagcat cataataatg gcaagtaccc aggcaaaaac cgggcgatca ataaaatagt
     5401 tagccatgaa agtcccctta agcctgcaac gttattgttt cgattcggtg ctggcgtttt
     5461 cctggctgga ggaaattgca cgtgctttga tacccggacg aatgcgttgc aaaccggaaa
     5521 cgatcacccg atcgccagcc tgcaagccag aggtgacaac ccactggtcg ccgatggctt
     5581 tgctggcttc gatttcgcgt agctgcacga catcgtcttt atccagaatg agtgccgttg
     5641 ctttaccctg ggcgttgtgg gtgacgcctt cctgcggcac cagtaataca ttctggcggc
     5701 taccttcatc cactaatgcc gtgacataca tgccaggcag caagtcacca tttgggttgg
     5761 ggaaaatcgc ccgtaacgtc acggagcccg tggtttcatc caccgtcggg tcggagaatt
     5821 tcagcgtgcc ggtctggccg taacgtttac cattttccag attgagctgt actggcgtac
     5881 tgccctgaac ctgtttgatt tgcccactgg cgacctcttc tttcatgcgc aagaaatcct
     5941 gcaccgactg cgtgagatcg acataaatcg ggtccagacg ttgtacggta accagcgaat
     6001 ctgcctgatt agcggtaacg agtgcgccga cggtcaccga cgatttcccg ctgacgcccg
     6061 taatcggcga ggtgacattc gcgtattgca gattgatcgt cgcctgttca acagccgctt
     6121 cggcgacggt gacattggct tctgcttcat tcaactgggt gcgtgcggtg tcgtaatcct
     6181 gacggctaac atagttggtc ttcagcaacg atgcctggcg gttaaaggtg atgcgggcat
     6241 tgctggcggt agagagcgct ttcgccagcg aacctttggc ggagttcagc tctgcctgta
     6301 aaggcgcagg atcaatctga tacagcgaat cgccctggtt cactttatcg ccttcgataa
     6361 agttgcgttt aatgataata ccgcccacct ggggacgtat ctcggcaact tcataaggaa
     6421 cggttctacc gggcaattcg ctcaacacat tgaccgaacc gggggagagt gtgacgacac
     6481 cgacctcagg cgtcatggcg gcggcgtttt ccgccgattt gtcatcgcag gcggtgagca
     6541 tcgcgccgca gaataacaac ggtattaaca gttttcttct tctgttcatt ttattccctg
     6601 aaaattcttg agaaataacg gttctttaaa ttgcgcgcga ggagagatat gagggattta
     6661 cagaccagcg gcatagtggg gttgtcagcc agtaagatgg gatgcaagca cagtgatcga
     6721 catggtgagg tcaacgacaa aaaaaatgtt gccgttctgc caacagtttc tgccagcatt
     6781 cgggcaacga agggttctac tggtggatac acataccagg ggaataaagt cgttttcgtc
     6841 tctccttcaa taatcaccgg gggtattgaa atcagcatct gcatccctcg tcatgccagc
     6901 catcaatttc agctgcttat gtccttacta aaaataagat gtgataccca gggtgacgat
     6961 gtcgctcata cgtcttaatt tcagcttgta cataatgttg aatttctgaa tctttaatgt
     7021 ttgttgcgaa atgttgagcg ttgacgtgat attgctttca tttttgtttt tcagaatcag
     7081 ttgcataatt ttgaactgat tatgtgaaaa acataattgt gaattcttat ggggcaagtg
     7141 tttaccataa gtaatctcaa gaagattttt gatactttct ttacggctga caaaataaat
     7201 aggcgcttta gcttttagtt tactgatgtg ataaggtgcc aaaacaataa ttctctcggc
     7261 atctaatttc tccaggaatt taatcgcttc ttcatcaagg atatggttgt gataggtgtc
     7321 aatgatcacc ttgaagggtt tattgcctac ttttttgatc tctgacaggg aattgatttt
     7381 tatcatttcg tgattatctt tcaactgcca aaagccctgt aaaagaaaag aatctttcgt
     7441 catgagaaaa atcataactt gctccttagc cgttatcgtt tcgaacatgt catccttttc
     7501 attatttaca tccttgtccg aatcgttgtt caatatagta aacgcctata agaactttgc
     7561 ccttcgtgaa ctaatacgtc tattaacact aatcaatact tcagatttat ccatcacgca
     7621 ctccatatat tgatcctaag taagattcct ggttgttatc agtttgtaac cagtaaacaa
     7681 aaggttaact tttgcggatt actgcccaag aataagttat caataaccag tttgtaatct
     7741 ctgaagaata ttactaaagt taaaatgtaa tccgatttaa atatcgagtc tccttgtttc
     7801 gacttaggct ggcaattgga ttgccagctt tcttttaacg cattgaaatt acctgattaa
     7861 ataactcatt catgtcttca ggtttttata tagcgtctgg agacacggat atttatgcaa
     7921 tgtaatcaac gccagttgca aaaaaatgaa cccggattac ttatttaacc gcccatagca
     7981 tcaacgctta cggaactctt tgttcacttt cccacagcac ttttaaaaca ttaaacctga
     8041 ccacggaaaa tatcagccat gataatgttt gaaatggcta atttgccatg gaacgaaaaa
     8101 aatcagatta aattcagtag gttgaaataa tcaacagcag gcaattgttt caatgatagt
     8161 gcgtaattga actgaaacac tgtatagcta agagagtatt aactggatca tagtcactta
     8221 atgtgactat gatccaggtg gaaataggga taattattgc tttttaacga acaaactggc
     8281 gaagctgaac agactggcgg cgctaaatat cagttcaatt cccaccagtg tggaaaccag
     8341 cgttaccgac accatcggcg tagcaccaag gaatatccag gcaatgacga tatccagcac
     8401 accaataacg agctgtagcc aactgccttt cattgaacgc tgacgatacc aactcatcag
     8461 gcgaataacc cctgccacac agaacaaacc ggcaataaat gccgcaatgg caaaaatgcc
     8521 cagctctggt gcgcggatga agaaatagcc gatcaataaa taggcgactg cgacgaggaa
     8581 accggataat accggccaga aattatgact gcggttgctg aatagcccga caataagcgc
     8641 aatgcccgag cagattaata atgcacccac tactgtgctt aaaatgtcgc cagagacgaa
     8701 cggaaaactg atacacagca acccgacgat aaacagcagt acggcaataa actggattgc
     8761 tctgcgatgt tttttaagca tctccagatc aaacttcaaa attgttgcct tatctatata
     8821 taacatagaa ccaccctata aaattaagaa gaaaatcccc tgctatcaat ctatgccaaa
     8881 aacgcgtgta agaatgcagt cgatttaata aaaatttcct aattacagta tctgatgcgt
     8941 ctgtaactca ttgtattgaa ataaaaatat ctgattttga tattttccat caacatgaca
     9001 tatacagaaa accaggttat aacctcagtg tcgaaattga ttcgtgacgg ctctttcact
     9061 ttatagttga ggatattacg atgaaaaaag tattaggcgt tattcttggt ggtctgcttc
     9121 ttctgccagt agtgagcaat gcagcggatg cacaaaaagc agctgataac aaaaaaccgg
     9181 tcaactcctg gacctgtgaa gatttcctgg ctgtggacga atccttccag ccaactgcag
     9241 ttggttttgc tgaagcactg aacaacaaag ataaaccaga agatgcggtt ttagatgttc
     9301 agggtattgc aaccgtaacc ccagctatcg ttcaggcttg tactcaggat aaaaaagcca
     9361 gctttaaaga taaagttaaa ggcgaatggg acaaaattaa gaaagatatg taattccggg
     9421 aatgcgttac atcgtatttc cttgcatatt gaacaggccg gaatatcttc tttaaaagca
     9481 gctattcctc ctgtttgtat ataatctcta tattgaatgg gttacaaaat gaacatttca
     9541 tctctccgta aagcgtttat ttttatgggc gctgtagcgg ctttgtcact ggtgaacgca
     9601 caatctgcgc tggcagccaa tgaatccgct aaagatatga cctgccagga atttattgat
     9661 ctgaatccaa aagcaatgac accagttgcg tggtggatgc tgcatgaaga aacagtatat
     9721 aaaggtggcg ataccgttac tttaaatgaa accgatctca ctcaaattcc taaagtgatc
     9781 gaatactgca agaaaaaccc gcagaaaaat ttgtatacct tcaaaaatca agcatctaat
     9841 gacttgccga attaataagg tacaagtaaa aaggagtagc aagttgagcc atcttgctgc
     9901 tcctttttgc atttttatat gacagcagaa tttattatac gacttatact tgcggtaatt
     9961 gtctgtggcg ctattggcat ggaaaggcaa atgcgcggca aaggagcagg gttacgcaca
    10021 catgtattaa ttggcatggg aagcgccctg tttatgattg tttcgaaata tggttttgct
    10081 gacgtgctgt ctttaggtca cgtcggactg gatcccagcc gtattgctgc tcaggttgtg
    10141 acgggcgtcg ggtttatcgg tgcaggtaac attctcgttc gtaaccaaaa tattgtaggt
    10201 ctgacgacgg cagcggatat ctgggtgacc gccgccattg gtatggttat tggcagcggt
    10261 atgtacgaac tgggtattta tggttcagtg atgaccttgc tggtgctgga agtcttccat
    10321 caattaacct tccgcctgat gaacaaaaat taccatttac agctgacatt agtgaacggt
    10381 aatacggtat caatgctcga ctggtttaag cagcaaaaaa taaaaacgga tttagtttcg
    10441 ttgcaggaaa atgaagatca tgaagtggtc gccattgata tccagcttca tgccacgacg
    10501 tcgatagaag atctgttgcg attattaaaa ggtatggcag gggtgaaagg cgtttcaatt
    10561 agctaatctg tatagttatt tattcgcagg agtaccagtg cggtactcct gttggtttta
    10621 atgatcgagg acgatcagtg gtgaatggtt ggcaggatct ttaagtacgg taatatccgc
    10681 gccgtagagc attgtcagcg cctgctgtgt caatacgtcc tgcggcttac catttgcgat
    10741 aacctttcct ttttgcatta atacaacacg gtctgcataa cgcgcggcaa gatttagatc
    10801 gtgcaatacg cagcaaacat tgaactgccg ttcgtgaact aactggcgta ataaacgaaa
    10861 caagtgctgc tgatggtgaa tatccagcgc cgaagttggt tcatcaagaa ataaccattt
    10921 cggtgaggga gtaggttccc agagttgcac cagcaggcgc gctaattgca cgcgctgttg
    10981 ttcaccaccg gataattgcc gatagtcgcg gtttgctaac gcctgacagt cacataacgc
    11041 cattatttgt gctgtttcat tatcctgatt accagtacga tgcggatgcc gccccatctg
    11101 gataacttct tgtacgctga atggaaacgc catgtggcta ttttgacgca tcacggcccg
    11161 gtgtttcgcc agttcagtaa tcgaccattc atttaatggc ttattaaaca accgacattc
    11221 gccgctatca ggttgaagat agccggttag ctggcgaagc agcgtagatt ttcccgcacc
    11281 attaggcccg aggatggcaa caatttcccc accgggaaaa gtcagcgaga cattatcagt
    11341 cagacgccgc ccctgaaggc tataaaccag attctgggcg ctgatcatgt gatcctccgg
    11401 ttgcgcagaa tcaaccacat aaaataaggg ccaccaagca ggctggttaa taatcccacg
    11461 ggcatttctg ctggctgtac cagcgtgcgg gcaagcgtgt ctgccatcag caataaacag
    11521 gctccggcga gggcggaaca agggattagc cagcggtgat ttgccccggt agtcatgcga
    11581 atcagatgcg ggatcaccag gccaataaag ccgataatgc cgcttaccga tacggcagca
    11641 ccaacgagca gtgagctcac taacagtaat tgctggcgtt tacgcttcac gttcacgccg
    11701 aggtagtggg cttcttcatc accgagctgc agtaaattca gcgttccggc gagacaagtt
    11761 gttgcgataa tggccggtag gatgaatgaa ctggcaacca ataacgtcgg ccattgcgcc
    11821 tgtccaagat tgcccatcat ccacaatgtg agttgtcgta gctgctgctc atcgccgata
    11881 tagctcagga tgccgatcgc tgcgccgcac agggcattga tggcgatacc ggcgagtaat
    11941 aattgcatca tgctgccatc gcgatggcgc tgcgtgatga gaaaaatgat ggtgcagacc
    12001 actaaacttc cggcaatggc aaacaccatt tgctcataaa gcaccagcac cactgggaag
    12061 gagaagggca gcacaatagc aacgccaacc attaatgcag aaccgctact gacgccaagt
    12121 aatccagggt cagccattgg gttgcgaaaa agcccttgca taattacccc tgcggttgcc
    12181 aacgctgcac ctaccagcac cgccagcaaa acccgtggta gacgaatatt gagccagata
    12241 tcgcggtatt cctcatcaaa aagggcatca aaactgatct ttaacgctcc ctgactggca
    12301 ccaaacagca ccagagctag caataatagt gatagcccca taaagaccct ggcggaagag
    12361 agtgaatcct tgagcacgca aaactccgtt acgacattac ggcgcggaca taccgcgccg
    12421 ggggatcacg ccggtttcag gtccggttca atcagactgt agacctgctg gttaagtgct
    12481 ggcgcatttg ccagttcatg gatatgtgcg gcgacatcag cacggttaac aaaaccatga
    12541 cactcctgat tttgtatccg ttgtgctttg cccgttgccg caccatcaag cagaccgccg
    12601 gggcggagaa tggcataatc aagctggctg gtttgcaacc agctttccgc cagcgttttt
    12661 tcccgtaccg cctgaccaaa cgcagccttt gcccgctctg ataaaaacgg ccagctatcg
    12721 ccgcatccca aagaggtcac cagaatcatg cgactgatgc cagccttctc ggcctcatca
    12781 atcactgttc tgtgcgccag ataatcctga gctccgccca ttgttgagat gataagcgca
    12841 tccgggccag ctgcgcggca agcagccgcg actacgctgg catcacaggc gtcgcctgta
    12901 aaaacctgta cgccttgttg cgccagtttc gtggcggcat cggcatgacg aatgacagca
    12961 acgaccggac gttgttccgc cagcgccagt tcaagcgttc tggcacccac accttttccg
    13021 cctgcgccaa agagtagcca cggtgtcatc aggcgtgttc ctttaacgat actgccagag
    13081 catggaaggc gctgacttgt tcgctcagga gctgacgatg atcatcccta ccgaggaaga
    13141 ttttcagcat tgcgctgcct tccttattga aaaagagaat agaggcggtg tccatgccca
    13201 taaatttacg ttcgatcaag gcaatatgcg tgcagttttc tgctttgata tgcccggaca
    13261 tgccgtgctt tccgcgtagg ttgaaatagc cgtggcgatg gaaacccgaa ggcagttcgc
    13321 cgctaaattc gagtattaca tcggtagtat gtactaacgt ggtgacgttg ccccattcgc
    13381 agacggtatc ccagacagta tcgaatttat cacctgacac aacagtcgga gagggcagat
    13441 ttttcactac ctccagcagc gtggtgttgt attgttcggc gatcgcctcc agcgtaccgt
    13501 ctggctccgt tttcaaaaat tcctgtaacg agacatggct cataaatcac tcctgttatg
    13561 gtttttcaag cgcaattgct ggcgcattaa gtacctgaat cagttcatta agagactgca
    13621 aaatgttgct tgcccagaaa cgaccttcat tggtcaaccg cagacacgtt gacgcatcgc
    13681 ggcttaagcc tttttggtgc cactgggcca gtaacggcgc gagtttttcg gcatgtggcg
    13741 taagttcgtc cagcggtaca cgcgccgttt caatgcccga ctgcaaggta tgacgccact
    13801 gcgcgttacg ttcggcgtta cgcatgatca tcatcagcgg ttttttgcct gccgcgacgg
    13861 attcatgcca ggtctgaaga ttgcgttcgt tcatccagga gtacccatta atcgaaccgc
    13921 cggctccgga accaaaggcc agacaatcgg caccttgttt aatcagcagg ttatagagat
    13981 tgcgttcgcg tgtggtacgg ccccagtggc tgttactgat gcagcgccaa ccggcatcgt
    14041 ccataaaatc acatccttgc aggtaaagat cgcgacgttc tgccggagag ggcacggtag
    14101 tacgcccatt ttccacggct ttgcccagcg gtgtattggg caggacattg agcgcataga
    14161 gatcgacgcc gtcgagaccg atatcgcggg caatagccag atcttctccc caggtttgcg
    14221 catcctgacc cggcagacca aacagcaggt cacagaccat cgcggcacgg tcgcgtttga
    14281 ccaggctttc cataaacgca atagcggttg ggccatccga ggtgcgggcc attttcttgc
    14341 ggattttgct gttaaacgac tgaataccaa ttgagaagcg gtttgctcca gcatcaaggc
    14401 aagcatcgat tcgctcagcg tcaaaattca gtacccggcc ttcaatagtg atttcacaat
    14461 caggggccag tggcagcttt tctcgtaatg tggtgatgat tctggccaaa tcgtgtgctg
    14521 aaagcgcgga aggcgtaccg ccaccgaaat agaccgcgtg gatgggggca gactgatgca
    14581 atacgctgtc tgcttccatt tctatttcac gaatcagggc gtcggtataa tgagcacacg
    14641 catcgtcatt aaaacggttc tgataaaaac cacaaaacgt gcagtgtgtc gcacaaaaag
    14701 gaatgtgtag gtaaaccagt cgcttacgtg gtgataccgt ttgattgatc atctcttgcc
    14761 aggtctgtgc cagttgctct ttggcaaccg gaatggcccc acgaaacggc atcatggcgc
    14821 gcctgtcttt aaagggctga tcaccgtcta aagcaaaatg tggcgtgaga tccagggtgt
    14881 tatttgtgtt cattcgtctg atttaaa
//
LOCUS       DAZGJT010000108        14654 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZGJT010000108 DAZGJT010000000
VERSION     DAZGJT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.108"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..371
                     /locus_tag="ACHKFK_003850"
     CDS             <1..371
                     /locus_tag="ACHKFK_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9054093.1"
                     /translation="ITYKVQRDKKARELELANATITDMQVRQRDVAALDAKYSRELAD
                     ARAENETLRADVAAGRKRLQVAATCAKSTTGASSMGDGESPRLTADAELNYYRLRSGI
                     DKITAQVNYLQEYIRTQCLK"
     gene            573..1094
                     /locus_tag="ACHKFK_003851"
     CDS             573..1094
                     /locus_tag="ACHKFK_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO9054094.1"
                     /translation="MNIVPLNYKGEIVSFNTDGWINVTGVAERFGKRIDNWMRLAETL
                     EYVRALDEALTGKESQILHPSQSRYVKTSKARKDRGGGTWLHPKLSVAFARWCDARFA
                     VWCDLHIDSLLRGELTEQQKYEQACRIRDDRKSKASNGAREMARWRWDKPVIEANVEY
                     WREQLQLTLDIAC"
     gene            1900..2409
                     /locus_tag="ACHKFK_003852"
     CDS             1900..2409
                     /locus_tag="ACHKFK_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO9054095.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            2381..4309
                     /locus_tag="ACHKFK_003853"
     CDS             2381..4309
                     /locus_tag="ACHKFK_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO9054096.1"
                     /translation="MNISEQQLNNMMSAVTTALQPLIRALPVTPVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNSMGNDQIRTVNLIKSARVGYTKMLLGVAGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTITLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSAADNEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPSR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDVAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            4293..4499
                     /locus_tag="ACHKFK_003854"
     CDS             4293..4499
                     /locus_tag="ACHKFK_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO9054097.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAELVLGTTRRRRRPLGVRL"
     gene            4496..6088
                     /locus_tag="ACHKFK_003855"
     CDS             4496..6088
                     /locus_tag="ACHKFK_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO9054098.1"
                     /translation="MKRKPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPSQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMFGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     LFRTRFKAVSPKRVDTPGHGIGNRFLRAGVEVDRYGRAVAYHICEDDFPRSGSGRWER
                     IPRELLTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQGQKDNPLINILDKFSTWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIASRLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIDSGLSTYEKELALMGEDYQDIFRQQVRESAEREKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            6078..7583
                     /locus_tag="ACHKFK_003856"
     CDS             6078..7583
                     /locus_tag="ACHKFK_003856"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO9054099.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPEEVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVTGTEAAVFEGQSGIEAGLA
                     DELINASDAISVMAAALNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREHLA
                     TMLAGQPGMSVEQARAILTAAAPQQPVASTQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAAAPLADAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AGSGKAEPVSASTTALFEHFMANHSPAAVRGGVSQTSADGDADVKMLMAMP"
     gene            7620..7967
                     /locus_tag="ACHKFK_003857"
     CDS             7620..7967
                     /locus_tag="ACHKFK_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO9054100.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISQATPALTPLMLD
                     EASGKLVVWDGQKAGSAAGILVLPLEGTETVLTYYKSGTFATEAIRWPESVDEHKKAN
                     AFVGTALSHAALP"
     gene            8025..9053
                     /locus_tag="ACHKFK_003858"
     CDS             8025..9053
                     /locus_tag="ACHKFK_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO9054101.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVVSSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            9105..9488
                     /gene="gpFI"
                     /locus_tag="ACHKFK_003859"
     CDS             9105..9488
                     /gene="gpFI"
                     /locus_tag="ACHKFK_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO9054102.1"
                     /translation="MATKEENLNRLRELSGLLGREADMSGSAADIAQRVSEWEEELAV
                     SPEGIMHSDESGADQNHTDDGEQLNNTDAPDDVKAVRVRKCLQVMGYCPETGRPVELA
                     LRGMRVLVPSSLATAMIQHGTAEYA"
     gene            9481..9834
                     /locus_tag="ACHKFK_003860"
     CDS             9481..9834
                     /locus_tag="ACHKFK_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO9054103.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGIRAQFTSGAQRGGEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEMFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            9850..10425
                     /locus_tag="ACHKFK_003861"
     CDS             9850..10425
                     /locus_tag="ACHKFK_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9054104.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMTINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNAKIIVNRGDLPVIKLGIRMLGRRPDSILKAGQ
                     HRYQRAFIQRLNNGRWHVMQRLPQARYEEGNDDKGRKKRNRLPIQVVKIPMAAPLKQA
                     FDENVDRIRRERLPKELAYALKQQLRIAIKR"
     gene            10422..10817
                     /locus_tag="ACHKFK_003862"
     CDS             10422..10817
                     /locus_tag="ACHKFK_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO9054105.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTAIPALAGLITTM
                     VTQGYEYRRDDDMALWSSADLTYSITYEM"
     gene            10825..11571
                     /locus_tag="ACHKFK_003863"
     CDS             10825..11571
                     /locus_tag="ACHKFK_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9054106.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKADAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEKGQKGLIAWFES
                     GDVRAYKIRFPNGTVDVFSGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAENLTKITP
                     VSGVTIKPETLTLEKGKAGTLTVAVLPDNASDPSLRVSTVHRAVATATLKENTVTVNA
                     LEKGTTSVVVMSDNGNFVALATVTVNVPAA"
     gene            11593..12024
                     /locus_tag="ACHKFK_003864"
     CDS             11593..12024
                     /locus_tag="ACHKFK_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO9054107.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEEAEATGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNALTDDDFSAGKPSTAS"
     gene            12051..12464
                     /locus_tag="ACHKFK_003865"
     CDS             12051..12464
                     /locus_tag="ACHKFK_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO9054108.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGARFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            12445..>14654
                     /locus_tag="ACHKFK_003866"
     CDS             12445..>14654
                     /locus_tag="ACHKFK_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9054109.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGVTSLAVATGALVYAWYQGDSTLSAFNKTLVLSGNQS
                     GLTADRMLTLSRAGQAAGLTFNQARESLAALVNAGVRGGEQFDAINQSVARFASASGV
                     EVDKVAEAFGKLTTDPTSGLMAMARQFRNVTAEQIAYVAQLQRSGDEAGALQAANDIA
                     TKGFDEQTRRLKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDE
                     ADKKWQWYQSRSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELAARD
                     RAERESSQLKYTGEAQKAYERLQTPLDKYTARQKELNKALKDGKILQADYNTLMASAK
                     KDYESTLKKPSGVKVSAGERQEDRAHAAMLALETELRTLEKHSGVNEKISQQRRDLWE
                     AENQYVVLKEAATKRQLSEQEKSLLAHEKETLEYKRQLADLGDKVEHQKRLNELAQQA
                     ARFEQQQSAKQAAISAKARGLTDRQAQRESEEQRLREVYGDNPAALAKATSALKNTWS
                     AEEQLRGSWMAGMKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMAAMLTGSEQNWR
                     GFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAANFH"
BASE COUNT         3517 a         3458 c         4501 g         3178 t
ORIGIN      
        1 ccatcaccta caaagtgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcca
       61 ccattactga tatgcaggtg cgccagcgcg atgttgctgc gctcgatgca aaatactcga
      121 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gctgctggtc
      181 gtaagcgcct gcaagtcgcc gccacctgtg caaagtcaac gaccggagcc agcagcatgg
      241 gcgatggaga aagcccaaga cttacagcag atgctgaact caattattac cgtctccgaa
      301 gtggaatcga caagataacc gcgcaggtta actacctgca ggagtacatc aggacgcaat
      361 gcctgaaata atttttttgc aaatcacaaa gtcaatttaa tgagcctcgc gatgcggggc
      421 tttttgcaat aaatgcgtac cgcaacgcat gttttttaca ccgaacctgc ccctttggaa
      481 tgggcctttg aggataccag ttagtgctgg cgagcctcgg tgggctggtt tcctatgcgg
      541 caaaggttca tttcaaatgg taggtaaacg ttatgaatat cgtgccactt aattacaaag
      601 gtgaaattgt cagtttcaac actgatggtt ggatcaacgt cacaggtgtt gctgagagat
      661 ttggaaaacg cattgataac tggatgcgtt tggcagaaac gcttgaatac gttcgtgctt
      721 tagacgaagc gttgaccggg aaagaatctc aaattttaca tccctcacaa tcgaggtatg
      781 taaaaaccag caaggcacga aaggacaggg gtggtggtac gtggctacat ccaaaacttt
      841 cagttgcatt tgcccgttgg tgtgatgctc gttttgctgt gtggtgcgac ctgcacattg
      901 atagtctgct tcgcggtgaa ctgactgagc agcagaaata tgagcaagca tgtcgcattc
      961 gcgatgaccg gaaatcaaaa gccagcaatg gggcaagaga gatggctcgc tggcgatggg
     1021 ataagccggt tattgaagca aatgttgagt actggcgcga gcaactgcag ttgactctcg
     1081 atatcgcgtg ctgatggcaa acgcaaaact gcgttatcgg aaaaatcaaa gcattacgag
     1141 aactgagcaa cggctatcca ttacaaagcc catctacggg tgggcttgat aatgaaaccg
     1201 gaatttattc ttggcaacca gttacggcag taccacgaaa caacccaagc cagtaagtgg
     1261 ggaaataaca ctggcagcca ctgaaagatg aagctcctgc cttaaggcaa aaaagattct
     1321 ttgtggtggc ggactgatgg aaagacatcg gttattgcag aggccattca atgagtggtc
     1381 tcgacaatgg cttataccct acacgggata acttaactga tatccctttt aaaggataaa
     1441 ggtattcaag cctgacacat catgcgctgt atcgtcgccg tattcctgca ttaacagaga
     1501 ccgcagcccg acggggaact cctctgcgcg agtgtgcgga aataatcaaa aacgatacac
     1561 accggggttt accgcgttaa cggagcgcgg cgttgtcccc tcatagtcgc ctgtccggtg
     1621 cgatggtgga agaaatcgga ctacattgaa aatgataacc attatcattt ttgcgggtcc
     1681 tttccggcga tccgggccgt tacggggcgg cgacctcgcg gattttcact atttatgaaa
     1741 attttccggg atccatgtcc ggtttctctg caagttaacc atatgaaaaa tataaaaaca
     1801 tgctttccat gaaccggaca tgcgcaaaaa atagacacta aaaccggaca tgaccggttt
     1861 tgttgtgatt gtgaggtgag agtttttgcg aggtgaggag tggctacgca gactgaagtt
     1921 gccaggcatt taagtctgac cgatcgccag cttcgcagat tgcagaaatt gccgggtgcc
     1981 ccgatatcga ataagcgagg gcaactggat ctggatgcct ggcgcgattt ttacatatcg
     2041 tatctgagaa gaagtaaaaa cgatgtgcct gatggcgata gcgaagacga ctatgaggag
     2101 aaattgctta ttgccagatg ggaactgaca gcagaacagg ctgttacaca gcagttaaaa
     2161 aatgaggtgt caaaaggaaa acttattgac accgggttct gtatttttgc cctcagcaag
     2221 ctggcaatgg cgttatccag tacgcttgat tccatccctt tatccatgca gcgacagttt
     2281 cctgatttaa caccgcgcca tcttgaccat ctgaaaaccc ttattgcgaa gggggcaaat
     2341 cagtgtgcgc gggcggggga taaattaccg gatttactcg atgaatatat cagagcaaca
     2401 actgaataat atgatgagtg ctgtcacaac agcattacag cccctgataa gggcattgcc
     2461 ggtgacgcca gttgaatggg ctgatcaaaa ttattatctg cctaaagaat cttcatatgg
     2521 tgagggagaa tggaaaacgc tgccgttcca gatcgccatc atgaacagca tggggaatga
     2581 tcagatccgg actgttaatc tgattaaatc tgcccgtgtt ggctatacaa agatgttgct
     2641 gggagtcgcc gggtatttta ttgagcataa atcccgaaac agtctgcttt ttcagcccac
     2701 ggattctgcc gctgaagatt ttatgaagtc tcacgtggag gcgacgattc gcgatgttcc
     2761 ctgcctgaaa aaactttctc cctggctggg acgtaaacat cgtgataata ccatcacgct
     2821 gaaacgcttt tcatcgggtg tgggcttctg gtgcctgggc ggcgctgccg ccaaaaacta
     2881 ccgtgaaaaa tccgtggacg tggtctgcta tgacgaactt tcctcgttcg agccggatgt
     2941 cgaaaaagag ggctcgccaa ccctgctggg ggataagcgt attgagggct cggtatggcc
     3001 aaaatccatt cgcggctcga cgcctaaaat aaaaggctcc tgccagatcg aaaaagcggc
     3061 caacgagtcg gcgcatttca tgcgttttta tgtgccctgc ccgcactgtg gggaggcgca
     3121 gtatctgaaa tttggcgatg agtccacgcc ttttggcctt aaatgggaga aggacagccc
     3181 cgaaagcgtt ttctacctct gtgaacatca tggctgcgtg atccatcagt ctgagcttga
     3241 ccagagcaac gggcggtgga tctgtgaaaa cacggggatg tggacccgcg acggtctgac
     3301 gtttttcagc gccgcggata atgaaattcc gccgccgcgc tccatcacat tccatatctg
     3361 gacggcgtac agtccgttca ccacctgggt acagattgtc tatgactggc tggatgcact
     3421 gaaagatccc aacggcctga aaacctttgt gaacaccacg ctgggcgaga cctgggaaga
     3481 ggccgtgggc gaaaaactcg atcaccaggt gctgatggat aaggttgtgc gttacacggc
     3541 tgcggtgcct tcccgggtgg tttatctgac ggcgggcatt gactcgcagc gaaaccgttt
     3601 tgagatgtat gtctggggat gggctccggg agaggaagcc tttctggtgg ataaaatcat
     3661 cattatgggg cgtcccgatg aggaagagac gctgttacgt gtggatgtgg cgatcaacaa
     3721 aaaataccgc catgcagacg gaaccgaaat gaccatttcc cgtgtctgct gggacaccgg
     3781 ggggatcgat ggcgaaattg tctatcagag gtcaaaaaaa cacggtgttt tccgggtgct
     3841 gccggtaaaa ggtgcatctg tttatggcaa gccggtgatc accatgccaa aaacccgcaa
     3901 tcagcggggc gtgtatctgt gcgaagtggg gacggacacc gcaaaagaaa ttctctatgc
     3961 ccgtatgaaa gccgatccca cgcctgcgga tgaagccacg tcgtatgcca tccgttttcc
     4021 tgatgatccg gagatttttt cgcagacaga ggcgcagcaa ctggtggcgg aagagcttgt
     4081 ggagaagtgg gaaaaaggaa agatgcgtct gctgtgggat aacaaaaagc ggcgtaacga
     4141 agcgctggac tgcctggtgt atgcctacgc ggcattacgt gtgtccgtgc aacgctggca
     4201 acttgatctg gctgtactgg caaaatcccg ggaagaagag acgacccggc caacccttaa
     4261 agaactggca gcgaagctgt ccggaggagt gaatggttac agtcgctgaa ctgcaggcgc
     4321 tgcgtcaggc gcgccttgat ttattaaccg gtaaacgggt ggtgtctgtc cagaaagatg
     4381 gtcgcagaat tgaatatacg gcggcctctc tggatgagct taaccgggcg atcaatgatg
     4441 ctgagttggt actggggaca acccgccgtc gccgtcgtcc gctgggagtg aggttatgaa
     4501 acgaaagcct gtcctgattg atgtgaacgg cgttccgctt cgggagagtc tcagctacaa
     4561 cgggggcggt gcaggatttg gcgggcaaat ggcagagtgg ttgccaccct cgcagagtgc
     4621 cgatgcggcc ctgctgcccg cgttgcgtct ggggaatgcc cgtgcagatg atctggtgcg
     4681 caataacgga atagcggcca atgcggtggc cctgcataag gatcacattg tcgggcatat
     4741 gtttctgatt agctaccgtc cgaactggcg ctggctgggg atgcgggaga ccgcggcaaa
     4801 aagttttgtc gatgaggtgg aggcggcctg gtcagaatac gcagaaggga tgtttggtga
     4861 gatcgacgtg gaagggaaac gcacgtttac ggaatttatc cgtgaaggtg tgggcgttca
     4921 tgcgtttaac ggcgaaatct ttgtgcagcc ggtctgggat acggagagca cgcaactgtt
     4981 tcgtacgcgt tttaaagccg tgagtccgaa acgggtggac acgccaggac acggtatcgg
     5041 gaaccgtttt ctgcgggccg gtgtggaggt tgatcgatat ggtcgtgccg ttgcgtacca
     5101 tatctgtgag gatgattttc ctcgctccgg gagtggacga tgggaacgga tcccgcgtga
     5161 acttctcacc gggcgtccgg ccatgctgca tattttcgag ccggtggagg acgggcagac
     5221 ccgtggggcc aatcagtttt acagcgtcat ggaacggctg aagatgctcg attccctgca
     5281 ggcaacacag cttcagtcgg ccattgttaa agccatgtat gcagcgacga ttgaaagtga
     5341 acttgatacc gaaaaggcct ttgaatatat cgccggtgcg ccgcaggggc agaaggataa
     5401 tccgcttatt aatattctgg ataagttctc cacctggtat gacacgaata gcgtgacgct
     5461 gggcggtgtc aaaattccgc accttttccc cggtgatgat ctgaaacttc agaccgcgca
     5521 ggattcagac aatggatttt cggcgcttga acaggcgctg ctgcggtata tcgctgccgg
     5581 tcttggcgtt tcctacgaac agttgtcccg tgattactcg aaggtcagtt attcaagtgc
     5641 ccgcgcatcc gccaatgagt cgtggcgcta ttttatgggg cggcgaaaat ttattgcgtc
     5701 ccggctggcc acgcagatgt tttcctgctg gctggaagag gcacttcttc gggggattat
     5761 tcgtccgcca cgggcacgtt ttgattttta tcaggcgcgt tcagcctggt cacgggctga
     5821 gtggattggt gccggaagaa tggccattga cgggctcaag gaggttcagg aatcggtgat
     5881 gcgtattgat tccggactga gcacgtatga gaaagagctg gcgctgatgg gcgaggatta
     5941 tcaggacatt ttccgccagc aggtcaggga atctgcagag cgggaaaaag ccggactctc
     6001 acgtccggtg tggatagcgc aggcgtatca gcagcagata gcggagagtc gcaggccgga
     6061 agaggagaca acaccacgtg agacgtaatc tttcacacat tattgcagca gcattcaatg
     6121 aaccgctgct tctggagccc gcctatgcgc gggttttctt ttgcgcgctc gggcgcgaga
     6181 tgggggcagc aagtctttcg gtaccacaac agcaggtaca gcttgatgca cccggaatgc
     6241 tggctgaaac ggacgagtac atggccggag gtaaacgacc ggcccgtgtt taccgggtgg
     6301 tgaacggtat tgctgtactg ccggtcacag gcacgctggt gcaccggctg gggggtatgc
     6361 ggccattttc cggaatgaca ggctatgacg gcattgtcgc ctgtcttcag caggcaatgg
     6421 cggatagcca ggtgcggggt gtactgctgg acattgacag tccgggcggg caggccgccg
     6481 gtgcgtttga ctgtgctgac atgatttacc gcctccgtca gcagaagccg gtctgggcac
     6541 tgtgcaatga cacggcctgt tctgcagcca tgctgctggc gtcggcctgc tcccgacggc
     6601 tggttaccca gacatcccgt atcggctcca ttggcgtgat gatgagccat gtcagctatg
     6661 ccggtcatct ggcgcaggcc ggtgtggata tcacgctgat ttactcaggg gcgcacaagg
     6721 tggatggcaa tcagtttgaa gcgttgccgg aagaggttcg ccaggacatg cagcagcgga
     6781 ttgatgcggc gcgccggatg tttgccgaaa aagtggcgat gtataccggt ctgtctgttg
     6841 atgcagtcac gggaacagag gccgccgttt ttgaaggtca gtccggcatt gaggccgggc
     6901 tggcggatga attaatcaat gcgtcggatg ccatcagcgt gatggctgcg gcgctgaaca
     6961 cacatgatac aggaggcact atgccgcaat taactgcaac ggaagctgcc gcgcaggaga
     7021 accagcgagt gatggggatc ctgacgtgtc aggaagcgaa aggacgtgaa caccttgcca
     7081 cgatgctggc aggacaaccg ggtatgagcg ttgaacaggc ccgggcgatt ctgaccgcgg
     7141 cagcaccaca gcagccggtg gcatccacgc agagtgaagc cgatcgcatt atggcgtgtg
     7201 aagaagctaa aggtcgtgaa caactggcgg caacgctggc ggcgatgccg gagatgacgg
     7261 tggaaaaagc ccgcccgatc ctggcggctg caccactggc ggatgccggg ccctcactcc
     7321 gtgatcagat catggccctg gatgaggcaa aaggggcaga agcgcaggct gaaaaactgg
     7381 cggcctgccc gggaatgacc gtggagaacg cccgggctgt gctggctgcg ggatcaggta
     7441 aggccgaacc ggtatctgca tccacaaccg ccctgtttga acatttcatg gcgaatcatt
     7501 caccggcagc ggtgcggggt ggcgtgtcac agacgtcagc agacggtgat gcggacgtga
     7561 aaatgctcat ggccatgcca tgaagtcagt gctgaccatc aatatgaggt tttaacaaaa
     7621 tggtgacgaa aaccatcact gaacaacgtg cggaagtacg tatttttgcc ggtaatgatc
     7681 cggctcatac cgccacaggc agcagcggga tttctcaggc aacaccggca ctgacgcccc
     7741 tgatgctgga tgaagccagc gggaaactgg tggtctggga cggacagaaa gccggtagtg
     7801 cggctggcat actggtactg ccgcttgaag gcacagagac ggtgctgacg tattacaagt
     7861 cggggacctt tgcgacggag gcaatccgct ggcctgaaag tgtggatgaa cacaaaaagg
     7921 ccaacgcctt tgtcggcaca gccctgagtc acgcggcgct gccgtaacac gttatcaggc
     7981 caccgcgttg gcctgactga tttctgaatg aaaggaactg atttatggga ttgtttacga
     8041 cccgccagtt gctcggttat accgaacaaa aagttaaatt tcgtgcgctg tttctggaac
     8101 tatttttccg ccgtacggtg aatttccaca ccgaagaggt gatgctggac aaaattaccg
     8161 gaaaaacgcc ggtggcggcc tatgtctccc cggtcgttga aggtaaagtg cttcgtcatc
     8221 gtggtggtga aacccgcgtg ttgcgtccgg gctacgtcaa gccgaaacac gaatttaatt
     8281 accagcaggc ggttgagcgc cttcccggtg aagatccggc tcagctgaac gacccggcct
     8341 accgtcgtct gcgtatcatc actgataacc tcaaacagga agagcacgct attgtgcagg
     8401 tggaagaaat gcaggcggtg aatgccgtgc tgtatggcaa atacaccatg gaaggggaac
     8461 agtttgacac ggttgaggtg gattttggac gctctgaagg aaataacatt gagcaggccg
     8521 acggtaaaaa atggtctgag caggaccgtg atacgtttga tccgacgcat gatattgacc
     8581 tttactgcga tcaggccagc ggtcttgtga atattgccat tatggacggt actgtctggc
     8641 gtctgctgaa tggctttaag cttttccgcg aaaaactgga tacccgtcgc ggctcaaatt
     8701 cacaactcga aacggcagtg aaagacctgg gggcggtggt gtctttcaaa gggtattacg
     8761 gtgatctggc cattgtggtg gcgaaaacgt cttatgtggc agaagacggt accgaaaaac
     8821 gttatctgcc ggagggtaca ctggtcctgg ggaatacggc agcagagggc attcgttgct
     8881 atggtgccat tcaggatgcg caggcgttgt ccgaaggtgt ggtggtctct tcccgttatc
     8941 cgaaacactg gctgacggtg ggggatcctg cccgtgaatt taccatgacg cagtccgcac
     9001 cgctgatggt gttgccggac ccggatgagt ttgtggtggt gcaggtgaaa taatccgtga
     9061 gcgggggcga aatgcccccg tgtctttttt cacaggagac cgagatggca acaaaagaag
     9121 aaaatctgaa tcgccttcgt gaactgtctg gcctgctggg gcgagaggcg gatatgtcgg
     9181 ggagtgctgc ggatattgct caacgtgtgt ctgagtggga agaggagctt gctgtttccc
     9241 cggagggcat tatgcactct gatgagagcg gggctgatca aaatcacaca gacgatggtg
     9301 agcagttgaa caacacggat gctccggatg atgttaaagc cgtccgggta cggaagtgcc
     9361 tgcaagtaat ggggtattgc ccggagacag gtcgtcccgt tgagctggcg ttacggggta
     9421 tgcgtgttct ggtgccatca tcactggcaa cggccatgat acagcacgga acggctgaat
     9481 atgcgtgatt ttcagaatgc ctttgatgct gccctcgccg gggtggacag tacgatcgtt
     9541 gaagtgatgg ggatccgtgc gcagttcacc tccggagcac aacgtggcgg cgaagttcag
     9601 ggggtttttg acgatccgga gtcgctgggt tttgccggtg gcggggtccg tattgaagga
     9661 agcagcccgt cattatttgt gcggacggat acggtgcgtg ccgtgcggcg tggtgacacg
     9721 ctgaccatta acggcgagat gttctgggtg gatcgtgttt ctccggatga cgggggaagc
     9781 tgttatctct ggctcaaccg tgggcaacca cccgctgtta accggcgacg ataaacgcag
     9841 ggtgaaatta tggcgataaa agggcttgat caggcgattg ataatctgag ccgggttcgt
     9901 aaaaacgcca ttccggcggc ttcagcaatg acgattaacc gcgtggccac aacggcgatt
     9961 aatcagtctt catcacaggt tgcccgggag acaaaggtac gccggaaact ggttaaggaa
    10021 cggtccagac tgaaacgggc cacggtcaga aatccgaatg caaaaattat cgttaaccgc
    10081 ggtgatctcc ctgtgattaa gctggggatc agaatgctgg ggcgtcgtcc ggacagcata
    10141 ctcaaagccg gtcagcatcg ttatcagcgg gcatttatcc agcgattaaa taatgggcgc
    10201 tggcatgtta tgcaacgtct tcctcaggcc agatatgagg agggcaatga cgacaaggga
    10261 aggaaaaagc gtaatcgcct tcccattcag gtggtgaaaa tcccgatggc ggccccactg
    10321 aaacaggcat ttgatgagaa tgttgaccgt atccggcgtg aacgcctgcc taaagaactg
    10381 gcatacgcgc tgaaacaaca actgaggatt gcgataaaac gatgaaacac actgacattc
    10441 gtgccgcagt gctggatgca ctcgagcagc atgaacacgg ggcgacgctg tttgatggtc
    10501 gccccgttgt ttttgacgaa gaggattttc ctgcgatcgc ggtttatctg acggatgcag
    10561 agtataccgg tgaagagctg gatgcagata cctggcgggc cacactgcat attgaggtgt
    10621 ttttaccggc acaggtacct gattcggagc tcgatcagtg gatggaaagc cggatttatc
    10681 cggcgatgac tgcgatccct gcactggcag gactgattac cacgatggtt acgcagggct
    10741 atgagtatcg tcgtgatgac gatatggcgt tatggagctc tgcggatctg acttattcca
    10801 ttacatacga gatgtgagga cgatatggca acaccaaatc ccctggagcc ggtaaaaggt
    10861 gccggtacca ctctgtgggt ttacaacggc aaggctgatg cttatgcaaa cccgttgtca
    10921 gacgatgact ggcagcgact ggctaaggtg aaggatctga cgccgggcga gatgacggct
    10981 gaatcctacg atgataacta cctggatgat gaagacgcgg actggaccgc gaccgggcag
    11041 ggacagaaat ctgcaggtga taccagtttt acgctggcct ggaaaccggg tgagaaaggt
    11101 cagaaagggc ttatcgcatg gtttgaaagc ggggatgtca gggcctataa aatccggttt
    11161 ccgaatggta cggtggatgt gttcagtggc tgggtcagca gtatcggtaa ggccgtgacg
    11221 gcgaaggaag tgatcaccag aacggtgaaa gttactaatg tgggacgccc gtcgatggct
    11281 gaaaatctca ctaaaattac gccggtttcc ggtgtgacca ttaaaccgga aaccctgacc
    11341 ctagaaaaag ggaaggcagg cacacttact gtggctgtgt tgccggataa tgcgtctgat
    11401 ccttcattaa gggtgtcgac cgtgcatcgt gcggtggcca cagccacgtt gaaggagaac
    11461 acagtgacgg taaacgcact tgagaaagga acaacgtcgg tcgtggtcat gagtgataac
    11521 ggtaattttg tcgctctggc gacagtaacc gttaatgtcc ctgccgcgta acgggtgttt
    11581 aggagaacac gcatgtttct taaaacagaa caatttgaat ataacggtgt gtctgtcacg
    11641 ctttcggagc tgtctgcgct gcagcgtata gagcatcttg ccctcctgaa acggcgggca
    11701 gaagaggctg aagccaccgg caacctgcag gtgagtgtgg aagatcttgt cagaaccggc
    11761 gcgtttctgg tggcgatgtc cctgtggcat aaccatccac agaaaacgca gtcaccatca
    11821 atgaatgagg ccgtgatgaa gatagagcag gaagtgctca ccacctggcc tgccgatgcc
    11881 attgcccggg cggaagacgt ggtgttgtgc ctgtccggga tgatcgaagc tgttcgtccg
    11941 gatactgata ttactgaagt ggcgaaaaat aacgcgctta ctgatgatga tttttctgcg
    12001 ggaaagcctt cgacggcgag ctgaattttg ccctcagact ggcgcgtgag atggggagac
    12061 ccgactggcg cgccatgctt gccgggatga catccaccga atatgccgac tggcaccgtt
    12121 tttaccgcac gcattatttt caggataccc agctggatat gcatttttcc gggctgacgt
    12181 acgctgtact cagcctgttt ttttgcgatc cggatatgca tccctctgat ttcagtctgc
    12241 ttgtcccccg gcatgaggaa gagcaggtgg agaggccgga tgaggacaaa atgctgatgc
    12301 agaaagcggc aggacttgcc ggaggcgccc ggttcggtgg ggacggaggg cgcgatattt
    12361 tatcgtctgc ggatgtggcg gatgtcatgg tggatgatgc cgcattaatg atggcttcag
    12421 cggggattcc gggaggtgtg agatatgtcc cagccggttg gtgatcttgt tattgacctg
    12481 agtctggatg ctgtccgttt cgatgagcag atgagccggg taaggcgtca tttttcaggt
    12541 ctggataccg acgccagaaa aaccgccagt gctgttgaac agggcctgag ccgccaggcg
    12601 ctggctgcac aaaaagccgg gatttccgtc gggcagtata aagcggccat gcgaaccctg
    12661 ccagcacagt ttacggatat cgccacgcag cttgccggtg gtcagaatcc ctggctgatc
    12721 ctgctgcaac agggcggtca ggtgaaggac tccttcggcg ggatgatccc catgttcagg
    12781 gggctcgccg gtgcgatcac cctgccgatg gtcggggtca cctcgctggc ggtggcgaca
    12841 ggtgcgctgg tgtacgcctg gtaccaggga gattccacgc tttcagcgtt taataaaacc
    12901 ctggttcttt ccggtaatca gtccggactg actgccgatc gtatgctgac gctctcaaga
    12961 gccgggcagg cagcagggct gacgtttaac caggcgagag agtcactggc agccctggtg
    13021 aatgccggtg tgcgtggtgg tgaacagttt gatgccatca accagagtgt cgcgcgtttt
    13081 gcgtctgcat ccggtgtgga ggtggataaa gtcgctgaag ccttcgggaa gctgaccact
    13141 gacccgacgt cgggactgat ggcgatggcg cgccagttcc gtaacgtgac ggcagagcag
    13201 attgcgtatg ttgcacagct gcagcgttcc ggagacgagg ccggggcatt gcaggcggcg
    13261 aacgatatcg ccacgaaagg ctttgatgag cagacccgtc gcctgaaaga aaacatggga
    13321 acactggaga cctgggcgga taaaacaggg aaggcattca aatcgatgtg ggatgccatt
    13381 ctggatatcg gtcgtccgga atcctcagcg gatatgctcg ccagtgcgca gaaggcattt
    13441 gatgaggcgg ataaaaaatg gcagtggtac cagagccgga gccagcgccg cggtaaaacc
    13501 tcctctttcc gggccaacct tcagggcgca tgggatgacc gggaaaatgc ccgtctgggt
    13561 ctggcagcgg caacgctgca gtcggatatg gaaaaagccg gtgaactggc ggcaagggac
    13621 cgggctgagc gtgagtcgtc acagctgaag tataccggag aggcgcagaa ggcgtatgag
    13681 cgcctgcaga cgccgctgga taaatatacc gcccgtcaga aagagctgaa taaggccctg
    13741 aaagacggaa aaatcctgca ggcggattac aacacgctga tggcgtcggc aaaaaaggat
    13801 tatgaatcga cgctgaaaaa gccgtcaggt gtgaaggtgt ctgccggtga gcgccaggaa
    13861 gaccgggcgc atgcagccat gctggcgctt gaaaccgagc tcaggacgct ggaaaaacac
    13921 agcggtgtga atgagaaaat cagccagcag cgccgggatt tatgggaagc ggaaaatcag
    13981 tatgtggtcc tgaaagaggc cgccacgaaa cggcagttat ctgagcagga aaaatccctg
    14041 ctggcccatg agaaagagac gctggagtac aaacgccagc tggctgacct gggcgacaag
    14101 gttgaacacc agaaacggct gaatgagctg gcacagcagg cggcgcggtt tgaacagcag
    14161 cagagcgcga agcaggcggc aatcagcgca aaagcccgcg gactcaccga ccgtcaggcg
    14221 cagcgggagt cggaagagca gcgccttcgt gaggtgtacg gtgataatcc ggctgcgctg
    14281 gcgaaggcca catctgcact gaagaacacc tggtctgcgg aggagcagct tcgtggaagc
    14341 tggatggccg ggatgaagtc cggctggggc gagtgggcgg aaagtgcgac ggacagtttt
    14401 tcgcaggtta aaagcgtggc cacgcagacc tttgacggta ttgcacagaa tatggcagcg
    14461 atgctgaccg gcagcgaaca gaactggcgt ggtttcaccc gttccgtgct ctccatgctg
    14521 acagagattt ttctgaagca ggcgatggtg gggattgtcg ggagtatcgg cagcgccatt
    14581 ggcggggctg ttggtggcgg cgcatccgcg tcaggcggta cagccattca ggctgcggcg
    14641 gcgaacttcc attt
//
LOCUS       DAZGJT010000109        14188 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZGJT010000109 DAZGJT010000000
VERSION     DAZGJT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.109"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(563..988)
                     /gene="arsC"
                     /locus_tag="ACHKFK_003867"
     CDS             complement(563..988)
                     /gene="arsC"
                     /locus_tag="ACHKFK_003867"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO9054110.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGITVRALLRKNVEPYEELGLAEDKFTHDQLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDTQKGAFTKEDGEKVVDEAGNRLK"
     gene            1522..1605
                     /gene="dinQ"
                     /locus_tag="ACHKFK_003868"
     CDS             1522..1605
                     /gene="dinQ"
                     /locus_tag="ACHKFK_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO9054111.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(1659..3011)
                     /gene="gorA"
                     /locus_tag="ACHKFK_003869"
     CDS             complement(1659..3011)
                     /gene="gorA"
                     /locus_tag="ACHKFK_003869"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO9054112.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTIEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNVEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(3083..3925)
                     /gene="rlmJ"
                     /locus_tag="ACHKFK_003870"
     CDS             complement(3083..3925)
                     /gene="rlmJ"
                     /locus_tag="ACHKFK_003870"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO9054113.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            4128..6170
                     /gene="prlC"
                     /locus_tag="ACHKFK_003871"
     CDS             4128..6170
                     /gene="prlC"
                     /locus_tag="ACHKFK_003871"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO9054114.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERIVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERNDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMELFKRFRGREPQLDAMLEHYGIKG"
     gene            6178..6930
                     /gene="rsmJ"
                     /locus_tag="ACHKFK_003872"
     CDS             6178..6930
                     /gene="rsmJ"
                     /locus_tag="ACHKFK_003872"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO9054115.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALSD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6979..8448)
                     /gene="dtpB"
                     /locus_tag="ACHKFK_003873"
     CDS             complement(6979..8448)
                     /gene="dtpB"
                     /locus_tag="ACHKFK_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO9054116.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADKFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            8614..8675
                     /locus_tag="ACHKFK_003874"
                     /pseudo
     CDS             8614..8675
                     /locus_tag="ACHKFK_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8765..9199)
                     /gene="uspA"
                     /locus_tag="ACHKFK_003875"
     CDS             complement(8765..9199)
                     /gene="uspA"
                     /locus_tag="ACHKFK_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO9054117.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            9590..9925
                     /gene="uspB"
                     /locus_tag="ACHKFK_003876"
     CDS             9590..9925
                     /gene="uspB"
                     /locus_tag="ACHKFK_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO9054118.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9996..11495)
                     /gene="pitA"
                     /locus_tag="ACHKFK_003877"
     CDS             complement(9996..11495)
                     /gene="pitA"
                     /locus_tag="ACHKFK_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO9054119.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     VGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            11727..12920
                     /gene="yhiN"
                     /locus_tag="ACHKFK_003878"
     CDS             11727..12920
                     /gene="yhiN"
                     /locus_tag="ACHKFK_003878"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO9054120.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVAFRLRSEVLSVAKDDTGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQTLISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLVAE"
     gene            complement(13007..14059)
                     /gene="yhiM"
                     /locus_tag="ACHKFK_003879"
     CDS             complement(13007..14059)
                     /gene="yhiM"
                     /locus_tag="ACHKFK_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HFO9054121.1"
                     /translation="MNIYIGWLFKLIPLLMGIICIALGEFVLTGSGQSEYFVAGHVLI
                     SLSAICLALFTTAFIIISQLTHGMNKFYNRLFPVIGYAGSATTMIWGWSLLASNNVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNASGSKSDGTPLQAYSSTIGNCLI
                     AVPVLLTLFGFIWSVILLRSADITPHYVAGHVLMGLTAICACLIGLVATIVHQTRNTF
                     SVKEHWLWCYWVILLGSLTIIFGIYVLISSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLAAFLAEIAQTDMAYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         3679 a         3547 c         3665 g         3297 t
ORIGIN      
        1 agaacaggaa tggattttct tctacataca gtaaatataa tgcatcatat cttcctgata
       61 taattacctt tgacttccct tttatttcca tctggtaata tttgagtgca aaaaaggcga
      121 taataatatc gcaaaagaaa acgttaacaa ctatcgaaaa tgctcgtttt aaaaagttat
      181 gatgttctct gatagtgtgt gaagtgatat ttttgtgatg ttttagatat gtaataataa
      241 aataggttaa cgtatgttga aatcatttat tgtgtaaacc actgatagat cataatccag
      301 tacatcttta aacagctgca ctgtatcgct gataactaag tagctcactg atacaccatg
      361 ctggacgcga taattggaat ctcacgcagg gagtactgtg taaatagcaa aatagcctga
      421 tgtgctacga ctgtcaggcc tacaaataac atacaattca tcgaattatc ataactttgt
      481 aggtcggata aaacgcgcca gcgtcgcatc cgacataaat aaagcgcatt tttctaacaa
      541 cctgtggggg aatatagccg ctttatttca ggcgattacc cgcctcatca accactttct
      601 cgccatcttc cttggtgaac gcgccttttt gcgtatctgg cagaatttcc agcacgactt
      661 ctgaagggcg gcacaggcga gttcccagcg gcgtcaccac aatcgggcga ttaatcagaa
      721 tcgggtgctg aagcataaag tcgattaact gatcgtgagt aaatttatct tctgcaaggc
      781 ccagttcctc atacggctcg acgtttttac gcagcaacgc gcgtacggta atccccatat
      841 cggcaatgag ttttaccagc tcatcgcgcg tcggtggtgt ttccagataa tagataatgg
      901 tcggttctgt accgctgttg cggatcatct ccagcgtatt acgcgacgta ccgcaggccg
      961 ggttgtgata aatggtaatg ttgctcatat cagtatctca ttacgatgtg aaagagaggt
     1021 gtagcaccag cgccaccaga gttacaaacc aaagcggaca agtcaaaaac acatatggtt
     1081 taacgaatat gtaagtgtag agaaaggtgt aggtgtgaat taagcgttgg gcattcattt
     1141 gaaaggaggt ctgaatcagg acgtaaaaaa accgacttcg cgtcggtttt tttactgttc
     1201 agccctgagt tggtggctct ggctggagtg agatagccat catctaacgc atcaatgcta
     1261 ataccatact gaaactattg caaggacatg ctggttttat aacccgcaag tactgtatga
     1321 ttatccagtt agttctgaga cattttcact ctggcaatgc gcataaacgc tttcaaagtc
     1381 ctggtctgaa gtacgggtgg tgccgttaac tgatgctctg gccggagtga gatagtcgtc
     1441 atctaacaat gagacatgcg ccgtgacagg caatggatga gtaagcggat gcattctcac
     1501 tccatcgcat ggagaaaacg ggtgattgat aaagcaatca tcgttcttgg ggcgttaatt
     1561 gcgctgctgg aactgatccg ctttctgctt cagcttctga actgatagcg gaaacgtagc
     1621 taagggctaa gagcacacta ctcttagccc tttaacattt aacgcattgt cacgaactct
     1681 tctgccgctg tcgggtgaat ggcaaccgtg ttgtcgaagt cttttttggt tgcacccatc
     1741 ttcagcgcca ccgcgaagcc ctgcaacatt tcgtccatac caaaaccaat gccgtgaata
     1801 ccgacaatct tctcttccgg tccaacgcat accagtttca tgcggcacgg ctggcggtga
     1861 gtggtgacgg cggtatacat cgcggtgaaa gaggatttat acactttcac ctgatcgtcg
     1921 ccgtactgct cgcgcgcctg cggctccgtt aaaccaacag taccaatcgg cggatggctg
     1981 aagaccacgg tcggaatgtt gctgtaatcc agatgctcat ccggcttgtt attaaacagg
     2041 cgttcagaga gacggcgacc tgctgcaaca gccaccggtg tcagttccac tgcacccgtg
     2101 ttatcgccca ccgcgtaaat accttcaacg ttggtgtttt gatatttatc gacgacgata
     2161 taaccttttt cgttagtttt aacgccagcg gcttccaggt tgatgttgtc attggcaggc
     2221 tcgcgaccaa tcgcccaaat caggcaatcc accgtttcac tgcgaccatc ttccagctcc
     2281 agcgtcagac taccatcggc atttttcact accgctttcg ggatggcgtt ggtgtgcaac
     2341 tgcgggcctt cggcgttcat cacttcaacc agcgtttcag agatcatcgg gtcgaagctg
     2401 cgcagcggcg catgtttacg cacaaacaga tgcgtcttcg caccgaggcc gttaatcacg
     2461 cccgccagtt caacggcgat gtaacccgcg ccaacaaccg ctacacgctc tggcaaagcc
     2521 ggaagggcga agaagccatc agaatcaata ccgtattcca cgcctggaat atccgggtgg
     2581 ctcggacgac caccggtggc gatcagaata tgatcggccg tgatggtttc gccgttgact
     2641 tcaatggttt tggcatcaac gaagcgggca aagcctttga ttacatcaac gttattttta
     2701 ccgagcacgt tctcatagga agtatgaata cggtcgatat aggcggtacg gctggcgatc
     2761 aacgtttccc agttgaattt attgatagtg gtatcaaaac cataatccgg gccgtacata
     2821 tggatcgctt cacggatttg cgccgcgtgc cacatcactt ttttcggcac acagccaaca
     2881 tttacgcagg tgccgcccag ctctttggct tcaatcagtg cgcatttctg gccgtacata
     2941 gccgcgcggt tgatggaggc gataccgccg ctgccgccgc cgatggcgat gtaatcatag
     3001 tgtttagtca tgacatattg tccttatcgt tgattaccgc gattgtagcg cgagacgtaa
     3061 taggtgccag caatggctgc aattactctg gcacgatcca gcttacggtg gcgtgcccgg
     3121 tgcctgccgg aaccagtttg ctgtgcagcc acggcagcac gttattcatc tgctgttcca
     3181 gcttccacgg cgggttaatc acaatcatgc cagaagcggt catgccacgg cgatcgctgt
     3241 ctggcagcac cgccagttca atttgcagaa ttttgcgaat gccggtcgct tccaggtcgt
     3301 ggatcatgcg cttaatttgc tggcgcagca ccactggata ccacaatgcg taagtgccag
     3361 tggcgaaacg tttgtaacct tctgctatcc cgctgaccac cgcctgatag tcggttttca
     3421 tttcatacgg cgggtcgata aggattaaac cacggcggga aaccggcggc agcttggcct
     3481 taagctgctg gaaaccgtcg gctttttcga cgcgcgcacg gctgtctttc tgaaattcag
     3541 aacgcagcaa cgggtaatcg ctcgggtgca gttcggtcag ttgcaggctg tcctgttcac
     3601 gcagtagctg gcgagcaatc aacggcgaac cggggtaata acgcaactgc ccgctacggt
     3661 tgaagtgttt taccacattg atgtacgcct ccagctccgc aggcaaatcg tcctgctgcc
     3721 agatacgggc gatgccttcg agatattcgc cggtacgctc ggcatgttcg ctgcctaact
     3781 gataacgccc ggcccctgcg tgggtgtcga gatagagaaa cggtttatct ttctctttca
     3841 gcgactcaat gatcaggctc tgaacggtat gtttaaggac gtcggcgtgg ttgccagcgt
     3901 gaaagctgtg gcgataactg agcatgggta aaggtgttcc ggtaagtaaa aatcggcccg
     3961 tattcggggc gcacaaaagc ctatcaggac agtataccga aaagaggccg ccgccgcgaa
     4021 agcgtaacgt ttctcattga aattcactac acttaacccc atgctacaaa cattatgtaa
     4081 agcgcctgtt gagcgcttcc ttaacctctt taaccaggac tgcgctaatg acgaatccgt
     4141 tactgactcc ctttgaattg cctccgtttt ctaaaattct cccggaacat gtcgttccag
     4201 ccgtgactaa ggcgctgaac gactgccgcg aaaatgtgga gcgcatagta gcgcaagggg
     4261 caccgtacac ctgggaaaat ctctgccagc cgttggcgga agtggacgat gtgctggggc
     4321 gtatcttctc cccggtcagc catctgaact cggtgaaaaa tagcccggaa ctgcgtgaag
     4381 cgtacgaaca aaccctgccg ctgctgtcag aatacagcac ctgggtaggg caacatgaag
     4441 ggctgtataa ggcatatcgc gacctgcgtg atggcgatca ttacgccacg ctgaacacgg
     4501 cgcagaaaaa agcggttgat aacgcactgc gcgacttcga actctctggc atcggtctgc
     4561 cgaaagagaa acagcagcgt tacggcgaaa ttgctacccg tctttctgaa ctgggcaatc
     4621 agtacagcaa caacgtcctc gatgcgacga tgggctggac caaactcgtt accgacgaag
     4681 cggagctggc ggggatgcca gaaagcgcgc tggctgcggc aaaagcccag gccgaagcga
     4741 aagagctgga aggttatttg ctgacgctgg atatcccaag ctacctgccg gtaatgacct
     4801 actgcgacaa ccaggccctg cgtgaagaga tgtatcgcgc ttatagcact cgtgcttccg
     4861 atcaaggtcc gaacgccggt aagtgggaca acagcaaggt gatggaagag atcctcgcgc
     4921 tgcgtcacga actggcgcaa ctgctgggct ttgaaaacta cgccttcaaa tcacttgcca
     4981 ctaaaatggc agaaaacccg cagcaggtgc tggatttctt aaccgatctg gcaaaacgcg
     5041 cgcgcccgca aggcgaaaaa gagctggcgc aactgcgcgc cttcgccaaa gccgaatttg
     5101 gcgtcgatga gttacaaccg tgggatatcg cttactacag cgaaaaacag aaacagcacc
     5161 tctacagcat cagcgatgaa caactgcgtc cgtacttccc ggaaaacaaa gcggttaacg
     5221 gcctgtttga agtggtgaaa cgtatttacg gcatcaccgc taaagagcgt aacgatgttg
     5281 atgtctggca tccggatgta cgtttcttcg aactgtatga cgagaacaac gaactgcgcg
     5341 gcagcttcta cctcgacctg tatgcccgtg aaaacaaacg cggcggggcg tggatggatg
     5401 actgcgtagg ccagatgcgt aaagccgacg gttcgctgca aaaaccggtc gcgtatctga
     5461 cctgcaactt caaccgcccg gtaaatggta aaccagcgct gtttacccat gacgaagtga
     5521 tcaccctgtt ccacgagttc ggtcacggcc tgcaccatat gctgacccgc attgaaaccg
     5581 ctggtgtttc cgggatcagt ggggtgccgt gggatgcggt cgaactgccg agccagttta
     5641 tggaaaactg gtgctgggag ccggaggcgc tggcgtttat ctccggtcac tatgaaaccg
     5701 gcgaaccact gccgaaagag ttgctggata aaatgctggc ggcgaagaac taccaggcgg
     5761 cgctgtttat tctgcgccag ctggagttcg gcctgttcga tttccgcctc catgccgagt
     5821 tccgtccgga tcagggggcg aaaatcctcg aaactctggc agaaatcaag aaactggttg
     5881 ccgtggtacc gtctccgtca tggggccgtt tcccgcatgc tttcagccac atttttgccg
     5941 gtggttatgc ggcaggttac tacagctacc tgtgggccga tgtgctggcg gcagatgcct
     6001 tctcgcgttt tgaggaagag ggcattttca accgtgaaac tgggcagtcg ttcctcgaca
     6061 acattctgag ccgtggcggt tcagaagagc cgatggagct gttcaaacgc ttccgtggtc
     6121 gtgaaccaca gctggatgcg atgctggagc attacggcat taagggctga acattcagtg
     6181 aaaatctgct taattgatga aacaggcgcc ggagacggtg ccttatctgt tctggcggct
     6241 cgctgggggc tggaacacga tgaagacaac ctgatggcgc tggtgttaac gccggagcac
     6301 ctggaactgc gcaagcgtga tgagccgaaa cttggcggca tctttgttga ttttgttggc
     6361 ggagcgatgg cgcaccgacg caaattcggc ggcggacgtg gtgaagcggt ggcaaaagca
     6421 gtgggcatta aaggcgatta tttgccggat gtggtggacg ccaccgcagg attagggcgc
     6481 gatgcctttg tgctggcttc ggtcggctgc cgcgtgcgga tgctggagcg taatccagtg
     6541 gttgccgcgt tgctcgacga tggtctggcg cgtggttatg cggatgcgga aatcggcggc
     6601 tggttacagg aacgtttgca gttgattcac gcctccagtc tgacggcgct gagcgatatc
     6661 accccgcgtc cgcaggtagt ttatcttgac ccaatgttcc cgcataagca gaaaagcgcg
     6721 ctggtgaaaa aagagatgcg cgtgtttcag tcgctggtgg ggccggatct tgatgccgat
     6781 ggattgttgg agcctgcgcg gttgctggca accaaacgcg tggtggtgaa gcgtccggac
     6841 tatgcgccgc cgctggcgaa tgtcgccacg ccaaacgcgg tagtcactaa agggcatcgc
     6901 tttgatattt atgccggaac gccggtgtaa aagtaaagcg gggtagcgat aacgctaccc
     6961 cgctttctgc aagaataatt aatggctttc cggcgtcgca atcatgcgtt tcagccacgg
     7021 caccatcaat agcatcacta ctgcaacgcc cagcgtaacc aggccaatct taccaaacac
     7081 gttggtatag acgggcaacg tctcaagtgg atcggtaatg ttgtctggta ctgcggtaaa
     7141 tgttgccaca tagccgccca gcaagaacgc ggcagcctgc gtcaggaacc acatcccgag
     7201 aataaagccc atcaaatgct gcggcaccag ggcagcaatc atcgccaggc caagggcgct
     7261 aataaacagt tcacctaagc tctggaataa gtacaccagc acgataaacc atggcgatgt
     7321 cagcccctgg gcatccgcaa accacattcc agccgctgca gccgtcaaaa agcccagtga
     7381 gcacataaac atgccgagag taaacttcat cggcatcgag agatctttgc ctttgttacc
     7441 cagatgcgtg taaatgcctg ccagtattgg gctggcgagt accacccaga acgggttaag
     7501 cgcctggaag ctgaccgggt tgatggaaaa gccgagaatt tcatgatgca cgttgttgat
     7561 ggcaaagaag ttcagcgacg ttggcatctg ggcgtagaga atgtaaaaca ccaccgcttc
     7621 gagcatcagg acaaaggcga caaacatttt attgcgcccg gttttatcca gcttgaatgc
     7681 ctgacgaaag aagatgatgg tgacgacgat ggagagaaca atcagaacca gattggcgac
     7741 ttctacgttg tgcatcagcc aggcgcagac gaagatcatc accacgctgc caagtaatac
     7801 gtacaacagt ttactgaagc tcatcggccg gaagtcgggt tcagaaccaa tgtctttcac
     7861 cattccacga caggcgatgt aaaccagtaa tgcgataatt aaccctgcac cgcacaggtt
     7921 gtaggtgact gaatagccga atttatcagc gatcacaggg gccagcgata acgctatcaa
     7981 cgagccgata ttgatcgaca tatagaacag ggtgaatgcg ccatcaagcc gcggatcttt
     8041 cggcgggtag cacttcgaaa gcaagctggc tgggttagct ttaaacaggc cgttaccgac
     8101 agcgatagtc cccagggcga tgaaaatcag gtcaggctta agtagcgaca tgccggtcat
     8161 gaagtagcca atcgccagca caagtgctcc gagaacaatg gtgcgtttgg ttcccagcag
     8221 gtggtcgcca acatagccac caatggaaat gaggccatag accagcgcag caaaagcacc
     8281 aaaagtgaca aaagcctgct cttgcgagaa cccaagctgt ttaacgaaga aaaccgccag
     8341 tacgccctgc acgccgtagt agccgaatcg ctcccataac tcgacaaaaa agatcatgaa
     8401 aaatgggcga ggttgctgca gcatccccat gggtgttgtt gtattcatat ttattaacct
     8461 tccaatacca tcctgaaaag tattaacgcg taagggataa cgcgcgaaaa agtttacatc
     8521 tgtcagagat tcttaatatt gatgcggaga atagattaat acactattac aacagaaaat
     8581 aaccagctga tcacactaat gatagaaaat atgatggtgt taaatgttgt attggcaggg
     8641 gttttgattg cgtacgtaga ccgcaggcca gataaggcgt ttacgctgca tcaggtaaca
     8701 aggcgcgcca aaaagcaaaa cgcccgcata tgcgggcgtt caggaacacg tcgtggaggg
     8761 aagattattc ttcttcgtcg cgcagcggaa caatcagcat atcaacgtga acggtgttga
     8821 tcagctgacg tgcggaagac atcagtttgc tccagaagtc ctggtggtga ccacaaacca
     8881 ccagatccat atcgtatttc ttgattgcat cgaccagtac ctggcccaga tcgccgctgc
     8941 cgctcagggt ttcagtgatt gggtagcctg cattggttga aagctcggtc agtgcatgat
     9001 gtgtctcttc agagatgcgt ttctgcatat cacccagatt cacatcgata agcccggtgt
     9061 ataggtcaga gtagtttaca tctacgtgga tcagagaaac tttcgcattg taggggcgag
     9121 ccatagagac tgctttctct accagaactt tgctttccgg ggagaggtcg accgcgatga
     9181 gaatgtgttt ataagccata gtgttactcc ttccataaag ttgtcgatga ctggccagct
     9241 agcgtttctt gtgccgttac gtcacccgcg ccctgcgaac tacgtgcgcg gggctaaccc
     9301 agccgtaaag attcagtgtt aagaccatcc ttaccttata gcgacccgga tgatccgtca
     9361 atccgccttg cttactcgtt agataaacaa attttcgaca tgaatatatt gatagtggtt
     9421 aaccttctgg aaaaaaaaca acctgatctc ctacactatc tatagagccg ctcggatgtt
     9481 ggtcaccaca aaagcggttt ctccaggacc tggtgttaac tgattggcag cgtatcggga
     9541 gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg ggaggaggta tgataagcac
     9601 cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt aacatggcgc gctatttctc
     9661 atcactacgc gcgttgttag tggtactgcg taactgcgat ccattgctct atcaatatgt
     9721 tgatggaggg ggctttttta cctcacatgg ccaacccaac aaacaggtgc gtctcgtttg
     9781 gtatatctat gcccaacgtt atcgcgatca tcacgatgat gagtttattc gccgctgtga
     9841 gcgggtgcgt cggcagttta ttctgaccag cgcattgtgt ggtctggtgg tggtcagcct
     9901 gattgcattg atgatttggc attaagcaat tcagcaataa tgacgcataa aaaaagcggg
     9961 ccagtaaact gacccgctcg ttttggtgcg tacaattaca ggaactgcaa ggagagccag
    10021 tacagcccgc cggaaagcag taccgcagcc ggaagggtaa acacccaggc catcagaata
    10081 ctggttacgg ttttacgctg taagccgccg ccatctacca ccatcgtccc cgcgacagaa
    10141 gaggagagta cgtgagtagt ggaaaccggc atcccggtat aacttgccag gccgatagac
    10201 acagccgccg tcatctgggc agacatcccc tgagcgtagg tcatgccttt cttaccgatt
    10261 ttctcaccga tagtcgttgc cacacgacgc cagccaatca tcgtaccgat acctaacgcc
    10321 agcgcgaccg ccatgatgat ccacaccggc gcatactcga tggtgctaag catgtcggac
    10381 ttcagtttct tcaacaggcg ctgatcgtca gcactcacgc caggcatctt caccacttta
    10441 tcgatagtgt cagaaacgca cagcataatg cggcgcatct ggctacgttg atcaagcgac
    10501 agcttgtcgt agctttccac atcggtagtc aacatgcctt tcaggcggtt gagcgcgtta
    10561 atggtattcg acggatggca atggaactcc gcgggttgcg ttgcgccagc ttccggagcc
    10621 ggtactaact gatcagcacc ggtcgcttgt ttcagcagcg caggatgctg ctcaaagtaa
    10681 gcttcgacgt tgttgatggc atcacgggta cgggtgattt cgtagccagt ggcattcatg
    10741 ttcaccacga agcctgctgg tgcgacacca atcaatacca acataaccag accaatgcct
    10801 ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga cagcggaaag gatcagcgca
    10861 atacgcgtcc agaacggcgg ctttttcttg ccgtctttct tttcacgctc cgctggggtc
    10921 aggtggatac gggcgcgttt cttggtgccg ctccagtagc gacgcagcaa gaaaatcaga
    10981 ccgccagcaa acaccaggcc gacaataggg gaaacgatca gagaaccgaa aatacttaat
    11041 actttcggga tattgagtgc atccaccact gacgtcccgg tcatcaacgc attggttaaa
    11101 ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg atgcaggtaa accaaagtac
    11161 caggtaccca ggttccagat aatcgccgcc agcaacatag agaacaccat ggcaaggcca
    11221 tgagacgatc ccatattaag cagcagatcc gtcggcagca tatgcacaat ggcataggca
    11281 acactcagac cacccagcaa aacacccaaa aagttgaata ccgccgccat aaccacggcg
    11341 agctgagaac gcatcgcgcg ggtatagata acggttgcca cggcgttggc tgtgtcatgg
    11401 aaaccattga tggcttcgta gaacagcaca aaagccagtg caagcaataa taacagcccg
    11461 gtatgcaaat ccaggccagc aaacaaatgt agcataggac gttacgccat tttgaggaca
    11521 tgaacgcggc gcattatcag tgactttcgc ggcgcgggca aagtgaaata tagacttttt
    11581 ttgatttgcc tcctgtatgg attccactca aaaaataatt atcttatata attcaggcaa
    11641 atacttcctt ttagtaatat tgatgctggt gcgaccactg aggaatcttt acaattcacg
    11701 cccgtttttt cttagaggag cgcaacgtgg aaaggtttga tgccattatt ataggcgctg
    11761 gtgcggcagg tatgttctgt tctgcgctgg caggtcaggc aggacgccgg gttctgctga
    11821 tcgataatgg taaaaaacca gggcgcaaaa tcctcatgtc tggcggtggg cgctgcaact
    11881 ttaccaacct ttatgtcgaa ccgggcgctt atctgagcca gaatccgcat ttttgtaagt
    11941 ctgcactcgc gcgttttacc cagtgggatt tcattgatct ggtcaataaa cacggcatcg
    12001 cctggcacga gaaaacgtta gggcagctct tctgcgatga ctccgcgcag cagattgtcg
    12061 acatgctggt ggatgagtgc gagaagggca atgtggcctt cagattgcgt agcgaagtgc
    12121 tgagcgtggc gaaggatgat acaggcttca cgcttgaact gaacggcatg actgtcggtt
    12181 gcgaaaagct ggtcattgcg accggtggac tgtcaatgcc ggggctgggc gcatcgccgt
    12241 ttggttataa gattgccgaa caattcggcc tcaacgtgct gccgacccgt gcgggtctgg
    12301 tgccattcac tctgcataaa ccgttgcttg aagagttaca ggtgctggcg ggcgtggcgg
    12361 tgccttccgt gattaccgct gaaaacggta ccgttttccg tgagaactta ctcttcaccc
    12421 accgcggctt atctggaccg gcggtgttgc agatttccag ctactggcaa ccgggtgaat
    12481 ttgtcagcat caatctgcta ccggatgtgg acctcgaaac cttcctgaat gagcagcgta
    12541 atgcacatcc gaatcaaagc ctgaaaaaca cgctggcggt tcatctgccg aagcggttgg
    12601 ttgaacggtt acaacaactc gggcaaatcc cggatgtctc gctaaaacag ctcaacgtgc
    12661 gtgaccaaca gacactgatt agcacattga ctgactggcg cgtacaaccc aacggcactg
    12721 aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga caccaacgaa ctctcttcac
    12781 ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat cggcgaagtg atggacgtca
    12841 ccggctggct ggggggctat aacttccagt gggcgtggtc gagtgcatgg gcttgtgcgc
    12901 aggatttggt ggcagaataa gaagcaaaat atgctctcga taccagaggg attaacctga
    12961 ataaaaccag ataaggcgtc caacgcctta tctgaagcct tttttattat tttttcgctg
    13021 aacccgcttc taatatagaa acgatggaaa acagtgtaaa acaaactgca cctaagccaa
    13081 ccagaactcg cgaaggaata aagtaagcca tatcggtttg cgcaatttca gccaggaacg
    13141 cagcaaggaa cagacaaaac agacaggtaa ataccggaat catgggtatt ctgttggcta
    13201 acgaacaggt gcgtcgccat accagcgcta acaaccacac tttggagaaa atactgtagc
    13261 agatcatccc caggcaaatg agaataatgc cgggggccag gcgggcgctt gcatctgaac
    13321 tgattaatac gtagatacca aaaataatgg taagtgagcc aagcaaaata acccaatagc
    13381 accacagcca atgttctttc actgagaacg tattccgcgt ttgatggaca atagtggcga
    13441 caaggccaat aagacaagcg caaattgccg ttaaccccat tagtacgtga cctgcaacat
    13501 aatgtggtgt gatatctgca ctacgtaaca gtatgactga ccaaatgaaa ccaaagagag
    13561 taagcagaac gggaacggca attaagcaat taccgattgt tgaagaatag gcctgcaatg
    13621 gcgtaccgtc actcttactg cccgaggcgt ttttggggat caacagaaaa tgtccggaag
    13681 atgccgctac ggttgataca caagcggcaa tcatgccaac gccgaaaata acatgaccgg
    13741 cgacaaattc atctgccatc acattattgc ttgccaacag tgaccatccc caaatcatag
    13801 ttgtagcaga accggcataa ccaataacag gaaataacct gttgtagaat ttattcatgc
    13861 cgtgagttaa ctgagatata ataataaatg cggtagtgaa caatgccaga catatcgcag
    13921 aaagagaaat cagaacatgc cctgcaacga aatattcact ttgcccagaa ccggttaata
    13981 caaactcacc taatgcgatg catattatac ccatcagtaa aggaattaat ttaaacaacc
    14041 atccgatata tatattcatt ttaaacccca tatatatttt ctgctacact tcgaaaaaca
    14101 tatgattcgt cttttcagtt agttaaatat gtctataaga tgccatcaat taaaaaaagt
    14161 aacttcacta taatcggatt tagcgtta
//
LOCUS       DAZGJT010000110        13858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZGJT010000110 DAZGJT010000000
VERSION     DAZGJT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.110"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(82..699)
                     /gene="yncG"
                     /locus_tag="ACHKFK_003880"
                     /pseudo
     CDS             complement(82..699)
                     /gene="yncG"
                     /locus_tag="ACHKFK_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310085.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            966..2465
                     /gene="ansP"
                     /locus_tag="ACHKFK_003881"
     CDS             966..2465
                     /gene="ansP"
                     /locus_tag="ACHKFK_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO9054122.1"
                     /translation="MSKHNTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETSS"
     gene            complement(2578..3639)
                     /gene="yncE"
                     /locus_tag="ACHKFK_003882"
     CDS             complement(2578..3639)
                     /gene="yncE"
                     /locus_tag="ACHKFK_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO9054123.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTNNQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3881..5983
                     /gene="pqqU"
                     /locus_tag="ACHKFK_003883"
     CDS             3881..5983
                     /gene="pqqU"
                     /locus_tag="ACHKFK_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO9054124.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAASQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDDASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNRGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLTDKLSLDAGVRYSSVWFDSNDHYVTPGNSDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVKA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYIPEDGWYAGKEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNFTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PAPGRNYGVGINIAWRFE"
     gene            complement(6066..6215)
                     /locus_tag="ACHKFK_003884"
                     /pseudo
     CDS             complement(6066..6215)
                     /locus_tag="ACHKFK_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            6464..6982
                     /gene="mddA"
                     /locus_tag="ACHKFK_003885"
     CDS             6464..6982
                     /gene="mddA"
                     /locus_tag="ACHKFK_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO9054125.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            6979..7428
                     /gene="ydcZ"
                     /locus_tag="ACHKFK_003886"
     CDS             6979..7428
                     /gene="ydcZ"
                     /locus_tag="ACHKFK_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO9054126.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLISGLVLDIFRSHGVPLRALIGPICGAILLVVGAWLVARRSF"
     gene            complement(7429..7662)
                     /gene="ydcY"
                     /locus_tag="ACHKFK_003887"
     CDS             complement(7429..7662)
                     /gene="ydcY"
                     /locus_tag="ACHKFK_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO9054127.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(7748..7921)
                     /gene="ortT"
                     /locus_tag="ACHKFK_003888"
     CDS             complement(7748..7921)
                     /gene="ortT"
                     /locus_tag="ACHKFK_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO9054128.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            8116..8211
                     /gene="yncL"
                     /locus_tag="ACHKFK_003889"
     CDS             8116..8211
                     /gene="yncL"
                     /locus_tag="ACHKFK_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO9054129.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(8308..9732)
                     /gene="patD"
                     /locus_tag="ACHKFK_003890"
     CDS             complement(8308..9732)
                     /gene="patD"
                     /locus_tag="ACHKFK_003890"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO9054130.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVNAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTAPSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGSPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTLFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(9754..10548)
                     /gene="ydcV"
                     /locus_tag="ACHKFK_003891"
     CDS             complement(9754..10548)
                     /gene="ydcV"
                     /locus_tag="ACHKFK_003891"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9054131.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(10538..11479)
                     /gene="ydcU"
                     /locus_tag="ACHKFK_003892"
     CDS             complement(10538..11479)
                     /gene="ydcU"
                     /locus_tag="ACHKFK_003892"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9054132.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIIVRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            complement(11480..12493)
                     /gene="ydcT"
                     /locus_tag="ACHKFK_003893"
     CDS             complement(11480..12493)
                     /gene="ydcT"
                     /locus_tag="ACHKFK_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9054133.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGTPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGEMQANGT
                     IQAVQYQGAATRFELTLSGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVALV
                     EER"
     gene            complement(12511..13656)
                     /gene="ydcS"
                     /locus_tag="ACHKFK_003894"
     CDS             complement(12511..13656)
                     /gene="ydcS"
                     /locus_tag="ACHKFK_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO9054134.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLENPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMAKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKALDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRDQHNLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPQGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
BASE COUNT         3352 a         3588 c         3456 g         3462 t
ORIGIN      
        1 ttaattgcta ttcatagcaa acaaatggac gtccagttaa ctttgccgct gatttattaa
       61 cattaatctt cggcattatt gttaaataat ggcattcctt tttagtactt cttgtaactt
      121 aggtatctga catacagcat cagcgatcac gctgatattc gcgcattatc ctgaaaccat
      181 ttatgtcccg gtccccatat gcgcataacg cagagatagc agtccaccaa ggttagctgc
      241 tcgccaaacg cataaggttc agcagtgagc tgcgaattca gccagatata aagcgatttg
      301 cgatattcaa ttacattctt ctttaactgt tcaggtgcgt cagatgccca acgcttaggg
      361 taatcggcga aagtgaacgt tggatagaca ttagcaacca gccagaccaa tagccgttga
      421 aactgttgac gttcggcgcg cccaagaggt ggcgcaaggt ccgggcgacg atcgagaacc
      481 atcaatgcaa tcgccgctgt ctccgtcata atctcgttat ttttacagcg ccagtgtcgg
      541 aacctgacac aacggattca gggtttttaa taaatcgcgt gaagttccct catggtcaaa
      601 accactgaca tcaacgaact gataaggaat atcagccagt gttagcatca gctcactgat
      661 tgtagagccc cagccgggta cgctatacac cttaatcata tgcccctcac aatcttttaa
      721 agattaagtg taggacagca cacaggttat ccagcaaaag gatttatcat tttttcgtca
      781 gttgtataaa tccccttcag gattgtaagg ggatgttttg catcgtttat cgataacggg
      841 taggatgctg cgccgatagt tttataccca taaagaataa tggtgataac tatcatcgcc
      901 aggatgaata aacattgttc atggcaactt atatgacttt ttcattaaag caatcaggga
      961 gagcaatgag taaacacaac accgacactt cagatcaaca cgccgcgaaa cgtcgctggc
     1021 ttaatgccca cgaagagggg tatcacaaag cgatgggcaa tcgccaggta cagatgatcg
     1081 ccattggcgg cgcgattggt acgggtttgt ttttaggcgc gggggcgcga ctgcaaatgg
     1141 cggggcccgc actggcactg gtctatttga tctgcggcct gttttcgttt tttattctgc
     1201 gtgcattggg tgagctggtg ttacaccgcc cttccagtgg cagttttgtc tcttatgccc
     1261 gtgagttttt gggtgagaag gccgcttatg ttgctggctg gatgtacttc atcaactggg
     1321 cgatgacggg gattgttgat attaccgccg tcgctctgta tatgcattac tggggcgcgt
     1381 ttggcggcgt gccgcagtgg gtctttgcgc tcgctgcact gaccatcgtt ggcaccatga
     1441 atatgatcgg tgtgaaatgg tttgcggaga tggagttctg gtttgcgctt attaaagtgc
     1501 tcgccattgt aacctttttg gtcgtgggta cagtgttcct cggtagtggt cagccgctgg
     1561 atggcaacac cactggcttt catttaatca ccgataatgg cggcttcttc ccccacggtt
     1621 tgctgcctgc gctggtgttg attcagggcg tagtgtttgc ttttgcctcc attgaaatgg
     1681 tgggtacagc ggccggagaa tgtaaagatc cgcagaccat ggtgcctaaa gcgattaaca
     1741 gtgtgatttg gcgtattggc ctgttttacg tcggctccgt ggtgttgctg gttatgttat
     1801 tgccgtggag cgcgtatcag gcggggcaaa gtccatttgt gacgtttttc tctaaactgg
     1861 gtgtgccgta catcggcagc attatgaaca ttgtggtgct gaccgctgcc ctctccagcc
     1921 tgaattcagg tctgtactgc accgggcgta ttctgcgctc gatggcgatg ggcggttccg
     1981 caccgagttt tatggcgaaa atgagtcgtc agcatgtgcc gtatgccggg attctggcga
     2041 cactggttgt gtatgtcgtc ggcgtattcc tcaactatct ggtgccgtca cgcgtatttg
     2101 agattgtgtt gaacttcgcg tcgctgggaa tcatcgcttc atgggcgttt atcatcgttt
     2161 gccagatgcg cctgcgtaaa gcgattaaag aaggcaaagc agcggatgtc agttttaaac
     2221 tgcctggcgc gcccttcact tcctggctga cattactgtt tttactgagt gtcctggtgc
     2281 tgatggcgtt cgattacccg aacgggactt ataccatcgc ggcactgccg attatcggta
     2341 ttctgctggt tataggctgg tttggtgtgc gcaaacgcgt tgctgaaatt cacagcactg
     2401 cgccagtcgt cgaggaagat gaagaaaaac aggaaattgt gtttaagcct gaaacgtcga
     2461 gttaattgca aaattgcccg gctacgtgcc gggcaaattc agactactga cgaaggtgcc
     2521 ggatgcggcg tgataaaaat caggcaattc tttccgtttt caccctcgcc atatctatta
     2581 cagcgcaata cgaatcacat cgtccggctg ggtagcttcc tgctgtttag tggatttttg
     2641 tttcacactg acatacagcg ttttgccatc ggcagacagc gccaggctgt tcggatgagt
     2701 cggcgtatcg aacgttttca ccactttata gcttttcgcg tcaatcacac tgactttacc
     2761 tgcctgacga tgcgtcacgt aggcttcatt acgcgctggg ttaaacagca cagccagtga
     2821 ttccggtgcc gcaaccttcg ccagaatatt gccattacgg gtatcgacca ctaacacttc
     2881 tgccgcttta gaatcggtga taaatgcacg ctgattgttg gtatcaaggc tgatgttgat
     2941 aaagaagtgc tctttgccgt catccagcag ctttttacgg ctgaggattt tattgtcggc
     3001 ggtgtcgatg gtaatcaatt cgccgtcagc gttagtggtg taaagacgtt tgcctttgct
     3061 atccagcgcc agaccggtac tcattttacc ggtgttctgg atggcggttt tcagtttgat
     3121 attctcgcca tcaacgaccc aaatcacgct ctctttacca ataccactga tgtaaacggt
     3181 gttcgtggca tcatcagcta ccagctcacg tggttgcaac gggcgtacct cttccgtgcg
     3241 cttacgatca tccagcacca gacgaccttt cacttcgccc gttttggcat ctatcgccgt
     3301 gaccgcacta tttacggtgt taccaaacca caacgtctga gtcgtgttat tgatggtggc
     3361 accaaacggc ttgagatcgt tatggatcgc ctgcgtcact tccagagtaa ccggatcaag
     3421 acgataaacc acgccgcctt tatccagttt gcggctttgc gaagtggcga gccacagcgc
     3481 gttttcttgc tggctataag ccatttcgta ggcaccttta cctaccgctt tacgcagcat
     3541 ttcttctgcg gcctgcgtac tgaatgatga agcaacaagc aatgaaccta acagtaatga
     3601 accacgcagg cgcgatgaaa acagatgacg taaatgcatg acgactccct ttgatgaacc
     3661 gatatttttg ctgcttcaca atgcttcgct aagcgtaatc atcgttacgc ccttgttata
     3721 aatgggaatg gtaatcatta ttttctcaaa tgtggagcat ttcttaccgt tacgactaca
     3781 attttctcaa ttacttaacg tattgtgccg tttcgttttc cataaattta caatttcatt
     3841 accatgacgg tgttttccca ttcttaacct ggcttttttc atgaagattt tttccgtccg
     3901 acagaccgtt ttgcccgcac tgcttgtcct ttcccccgtt gtttttgccg ctgatgaaca
     3961 gaccatgatt gtcagtgccg catcgcaggt ggtttcagaa ctggataccc cagcagccgt
     4021 gagcgtggtg gatggcgagg agatgcgcct ggcaacaccg cgcattaact tgtctgaatc
     4081 actgaccgga gtgcctggtt tacaggtaca aaaccggcag aactatgcgc aagatttaca
     4141 gctgtcgatt cgcggatttg gctcccgctc cacttacgga attcgcggta ttcgcctgta
     4201 tgtggacggt attcccgcta ccatgcccga cggacaaggg caaacatcta acatcgattt
     4261 aagcagtgtg caaaatgtgg aagtgctgcg tggccccttc tctgctctgt atggcaacgc
     4321 gtctggcggt gtaatgaatg tcaccaccca gaccggacaa cagccaccaa ccattgaagc
     4381 cagtagttac tacggcagtt ttggcagctg gcgctatggg ctgaaagcta cgggcgcaac
     4441 gggcgacggc acacaacccg gcgatgtcga ttacaccgtc tcaaccacac gttttacgac
     4501 ccacggctat cgtgaccata gcggcgcaca gaaaaattta gccaatgcca aactgggcgt
     4561 acgcattgat gacgccagca aattaagcct gattttcaat agtgtggata tcaaagcaga
     4621 tgacccaggt gggctaacca aagcagaatg gaaggcgaat ccacaacaag cgcctcgtgc
     4681 agaacagtac gacacgcgaa aaaccatcaa gcaaactcag gctgggttgc gctatgagcg
     4741 tagcctgagc gcgcaagatg atatgagtgt gatgatgtat gccggagagc gagaaacgac
     4801 ccagtaccag tcaatcccga tggctcccca acttaacccg tcacatgcgg gcggcgtgat
     4861 tactctgcaa cgccattatc aggggataga cagccgctgg acacaccgtg gtgaactggg
     4921 cgttccagtc acgttcacaa ccggcctgaa ctacgaaaac atgagtgaaa accgcaaggg
     4981 ttacaataac ttccgcctga atcgcggcgt gccggaatac gggcaaaaag gtgagttgcg
     5041 ccgcgacgaa cgcaatttga tgtggaacgt cgatccctat ttacaaaccc agtggcaact
     5101 gaccgacaaa ctttcgctgg atgctggcgt gcgctacagc tccgtgtggt ttgattccaa
     5161 tgatcattac gttactccgg gtaacagcga cgacagcggt gatgccagtt atcacaaatg
     5221 gctacctgcc ggttcgttaa aatatgcaat gaccgatgcc tggaatatct atctggcagc
     5281 cgggcgaggt tttgaaacgc cgacgattaa tgagctgtct tatcgtgctg atggacaaag
     5341 cggtatgaac tttgggttaa aaccctccac caacgataca attgagatcg gcagtaaaac
     5401 gcgtattggt gatgggctgc ttagtctcgc attgttccag accgacaccg atgatgaaat
     5461 tgttgtcgat agcagtagcg gtgggcgtac gacttacaaa aatgccggaa agacccgtcg
     5521 tcaaggcgct gaactggcat gggatcaacg tttcgcggga gattttcgcg taaaagcgtc
     5581 ctggacctgg cttgatgcga cctatcgcag caatgtgtgc aatgaacagg attgtaacgg
     5641 taaccggatg ccagggatcg cccgtaatat gggctttgcg tcgataggtt atataccgga
     5701 agatggttgg tatgcaggca aggaagcgcg ttatatgggc gatattatgg cagatgatga
     5761 aaatacggcc aaagcgcctt cttatacact cgtcggctta ttcaccgggt ataaatacaa
     5821 ttaccacaat tttaccgtgg atttatttgg tcgtgtcgat aatttattcg ataaagaata
     5881 cgttggctct gtcattgtca atgagtcaaa cgggcgatat tacgaacctg cgcccggacg
     5941 aaattatggt gtcggcataa atattgcgtg gcgatttgag taattatctt taagcccggt
     6001 atttgccggg cgtattttat ttatcggctg tattgttggc acaacaaagc aacgtttttt
     6061 tgcatagtga ggttttcgac agccagagca actgccatta attccagttc gtagcggatc
     6121 cgattgattt catttaattg acgttttccc acttccggaa ctgtaaatgc ttgcgctggt
     6181 gcgacaaaga gcgcatttac cgaaaccaga cgtaaggaac cggcgcgcca tgcggacgca
     6241 aggacagaac ccaacatcgg ttacgctgcg tttgttgccc cataatgctc tcctgtgctt
     6301 ttggcatgaa tccatcaata gtagcaggct cacaggattg gttttgtttg cagccgtcac
     6361 atcccctctg caacacgcaa taatttcata gaggaatccg ttgcttcggg ctgttttcga
     6421 tagtattgat aacatcatca tcattatttt atcgaggcca gacatgtcca tccgttttgc
     6481 ccgcaaagcc gactgtgctg caattgcgga aatttataac cacgccgtgt tgtatacggc
     6541 ggctatctgg aatgaccaaa cggtggatgc ggataaccgc attgcctggt ttgaagcgcg
     6601 gaccatagca ggttatccgg tgctggtgag tgaagaagat ggcgtagtga cgggatatgc
     6661 ctcgtttggc gactggcgta gttttgatgg ttttcgccat accgtggaac attcggttta
     6721 tgtccatccc gatcatcagg ggaaaggtct ggggcgtaaa ttgttaagcc gattgattga
     6781 tgaagcgcgg gattgcggga agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc
     6841 ctcgctgcat ctgcaccagt cgctgggatt tgtcgtcacc gcgcaaatgc cgcaggtagg
     6901 tactaaattt gggcgctggc tggatctgac atttatgcag ttgcaactcg acgagcgcac
     6961 tgaaccggac gcgatcggat gaatcagtca cttacccttg cctttttgat tgcagccggg
     7021 attggtctgg tagtccagaa cacgctgatg gtgcgcatca cccagacctc ctctaccatt
     7081 ctcatcgcca tgttgctgaa ctcactggtg gggattgtgc tctttgtttc tattttatgg
     7141 tttaaacaag gcatggcggg gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt
     7201 atacctggct tgctgggatc gttttttgtc ttcgccagta tcagcggata tcagaatgtg
     7261 ggagcggcga caactattgc agtactggtc gccagccagc tgattagcgg tctggtgctg
     7321 gatatcttcc gcagccatgg cgtaccactt cgggcgttga tcggcccaat ttgtggcgca
     7381 attttacttg tggttggcgc ctggctggtg gccagacgct cattttaatc agaggatggt
     7441 accgcctttg gttaactgtt cgtggcgcgc ttccatatct tctttatgtt tgcgaccgtg
     7501 atgagcaata gcggtgcgca aacgctgctg ctgggtgtag cgatcttccc gacttaactc
     7561 cgcgtcatcg ctcagggcga ttaataattc gttcatatgg gcaatgacgc tctcttcaat
     7621 cgcggcatcc acgcgcgcga tgacttcatc cagatgcgac atactttctc cttagccact
     7681 tgtgaaattt atcgattcat ccaaatgata taagtctgcg tggtgccgtc aagccagtgc
     7741 ataagattta aaacagagaa aacaacagcg ccaccgggaa actcatcggc catgttgccc
     7801 ccaccagaaa ggcgcttaag aaacggatgc gtttcttatc gtgagaaaga aaccaggtga
     7861 taagaaatgc gattgctgcc ataaccgcat aaaaaacaag catatgttga tagagagaca
     7921 tgtacgggcg tccagccaga tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg
     7981 catcaagttt tttcaccagt tagagaaaaa atcatcaaac agcaacttgc ccttatctat
     8041 cttcagaatg agtattattg cggattttct taacccgtac tatacacata ggggcaatga
     8101 taaaaggtgg caaaaatgaa tgtttccagt agaactgtag tactgataaa tgtctttgct
     8161 gctgttggtt tgtttactct tatctctatg agatttggct ggtttatttg atgttgtgat
     8221 tttgcacgca gcacgtactg tcagagaaat cagtgctctg acagtacgca agatcatctc
     8281 atactcttac gatctgcaat attatcctta atgtttaacc atgacgtggc ggacgacggt
     8341 gtaatcctcc agcccataaa gtgacatatc cttgccgtaa ccagaaagtt tctgcccacc
     8401 gtgcggcatt tcacttacca gcatgaaatg ggtattgacc caggtacaac catattgcag
     8461 ccgcgcgctg acgcgatgcg ccctgcccac atccttcgtc cataccgaag atgcaagtcc
     8521 gtactgactg tcattcgccc agttcaccac ctgttcttcg ttgtcgaaga gcgtaacact
     8581 cactaccgga ccaaatacct ctttttgcac gatggcatcg tcctgtaatg cgccagccag
     8641 cagcgtcggc gcatagtaat agccattacc cttgcgcttt tcaccgccag tgatcacttt
     8701 gatgtgccct gtcgctttcg cctcttctac cgccttgctg acgcgttcga gatgcgccag
     8761 cgagcttaaa ggtccaagct ccgtagactc gtcatccggc gaacccgatt ttaacgttgc
     8821 caccgcagca ccgagttttt ccaccagcgt atcgtaaatg cctttttgcg cgtagatccg
     8881 acaagccgca gtacaatcct gtccggcatt gtaatagcca aaagtacgta caccttcgac
     8941 cactgcttca atatccgcat catcaaaaac aatcactggc gctttgccac caagttccat
     9001 atgagtacgc ttaatggacg gcgcggtatg gctgatgatg tgttccccag tggcgataga
     9061 acccgtcagc gacaccatcc gcactttggg atgtccggtc agcggatctc ccaccgtttt
     9121 gcctctgcca aacagcacgt taatcacgcc agccgggaag atatctttcg ccagctctgc
     9181 caacttcaac gcggtcagcg gggtaatttc tgatggttta agcactacgc agttccctgc
     9241 cgccagcgcc ggagccagtt tccacgcagc catcatcagc ggataattcc acggtgcgat
     9301 agaagccacg acccccaacg gatcgcgacg gatcatcgaa gtatgacctt caagatattc
     9361 acctgccgcc agaccattca ggcagcgcgc cgcaccggcg aaaaagcgaa agacatcgac
     9421 aatcgccgga atttcatcat tgaacgcact atgcagcggt ttgccacaat tacgggactc
     9481 cagttcggca aaaacctgac cattttcttc gataacatca gccagtttca gcaggcattc
     9541 cgcacgcgct tttggcgtgg tttgccccca ttcggcaaat gctgcatctg ccgcgcgaac
     9601 agcagcattg acctgctctg cggatgcctc ggcaatctcc agtaaaacgt cccccgttgc
     9661 cggattatag acaggctgtt tttccccttc gccgctaacc agttctccgt taatcagtaa
     9721 cttatgttgc atagcatatt cctgtatcag tggttattga ccattgtcgc cttcgcgggt
     9781 tagccaccag gcccccagga tcggcaaggt tgttaccaac ataaccagca gtgccaccac
     9841 gttggttacc ggtacatcac gcggtcgccc aagctgattg agcaaccaca acggtaacgt
     9901 tcgttcatga cctgccgtaa aagtcgtcac gatgatttca tcgaacgaca aggcaaacgc
     9961 cagcattcct ccagccagta acgccgaact gagattcggc aacactacgt agcggaaggt
    10021 ttgccagcca ttggccccaa gatccattga cgcctcaacc agactccacg aggtgcggcg
    10081 aaaacgggcg atgacattgt taaacaccac cactacgcaa aaagtcgcat gaccgaccac
    10141 gatggtgaaa aatcccggct ccagattgat ggttttaaag gcggttaata gcgccagacc
    10201 agtgacaatg cccggcagcg caatgggcag caacagtaac agcgaaatgg cgtttttgcc
    10261 gaaaaagtct cgtcgccaca gcgcggcagc ggccagcgtc cctaacacca gcgcgattaa
    10321 tgtcgccagc gccgccactt taagtgacag tgtcacggca tcaagaatat cactacgctg
    10381 tgctgccacg ctaaaccagc gcagcgtcag gccttgcggt ggaaaactaa acgccgcatc
    10441 ttcagtgtta aacgcatagg cagcgataat caggatggga aaatgtagga aaaccacgcc
    10501 gccccaggcc gccagtttga ggaaaaacgg tgcgcgttca gagtgcatcg aacgctccca
    10561 gacgtttcac gaacgccagg taaagtgcga tgagaacaat cggcaccagg gtgaatgccg
    10621 ccgccatcgg catattgcca atcgccccct gctgggaata aaccatattg ccgataaaat
    10681 atcctggagg gccaaccagc tgcgggacaa taaaatcgcc cagcgtgagt gagaaggtaa
    10741 agatagagcc agcggcaata cccgggattg ccagcggcaa caccacatag cgaaaggttt
    10801 gtcgtggacg tgcgccaaga tcagccgacg cctgcaacaa tgacggcggc aaacgctcaa
    10861 gcgccgcctg cacgggcagg atcatgaacg gcagccagat atagagaaac accagaaagc
    10921 gccccagccc ggaagttgac agcgtattac ctcccaccgc cggtagcgtg aggaacgcag
    10981 tcagcagtgg ttccagccca agatgttgta aaaaccactg agccacgcca tcttttgcca
    11041 gcaataacgt ccaggcatag gctttaacaa tgtagctcgc ccacatcggc aacattaccg
    11101 caatataaaa aaacgctttc atttttccgc tggtatagcg cgccatatac cacgccattg
    11161 gaaaagccag aatggcgctg gcgatagtga ctgccacagc catggtcagc gtgcggacaa
    11221 tgatgtcgta attcgccgga ttaaacagcg cacggatatt cgccagcgtc agttccggcg
    11281 ttaccgacat ggtgaaatcg tcaaaggtat aaaagccctg ccataacagc gtcagcagcg
    11341 agccgaaata gacaatgcca aaccacatta gcgggccaag cagcagcaaa aacagcccca
    11401 gccccggatt acgccagaag aaaccggaca ccttacccag acctggacgt gaaggtgatt
    11461 gcaatacgtt catcgccatt cacctctcct caaccagcgc caccatcaca tcacgcgacc
    11521 aggaaaccat cacctgttgt ccgggcgtga gcgtggcagg cagttcttcg cctgtcatat
    11581 tggcctgact caccagcagt ttttcaccgc cgctcagtgt cagttcaaaa cgggtcgctg
    11641 cgccctgata ttgcaccgcc tggatcgtgc cattagcctg catttcgcca ggggtgttga
    11701 ggcggatatg ttccggtcgt agtgcgaagc ttcccgtcat gccacaaagt ttctctgcca
    11761 tcagtccatc aaaaacattc gatgtcccaa cgaatcccgc aacaaacggc gtgcgcgggc
    11821 gcatatagag atcgcgcgga gaatcgacct gttcaatgcg tccattattg aaaaccgcca
    11881 cccgatcgga catcgataac gcttcgccct gatcgtgggt aacgaagata aaagtgatac
    11941 cgagagactg ttgcagcttt ttcagctcaa gctgcatttg ctctcgcaat ttgagatcca
    12001 gcgcgccgag cggttcatcc agcaacaaca cacgcggttc gttcaccagt gctctggcga
    12061 tggcgactcg ctggcgctga ccaccagaaa gttgtgacgg tttacgttgc tgtacaaacc
    12121 ccaacgccac tttctcaagc gcctcttgcg ccattgcatg ccgctgcttt ttattcacgc
    12181 ctttgaccat cagcccgtag gctacgttgt caagaatcga catatgcgga aatagcgcgt
    12241 agtcctggaa gacagtattc acgtcccgct cccacggtgg cagattgctg gcgggcgtac
    12301 caaaaataga gatagccccg ccggaaagct gttcgaatcc agcaatcagg cgcaggcaag
    12361 tggttttgcc ggagccggac ggccccagca tagagaagaa ctcaccatct tttatcgcaa
    12421 tactgacgcc atctactgcg cgcacgtcac cgtacaaccg tgagacgttg tcaaactcca
    12481 ctgcgtacgt cataaaacac cccagcgaag ttagcgaccg cccataatgg caatgtaatc
    12541 ctgcgtccag cgactgtagg gaacaaactt gcccccttct gctataggcg ttttccagaa
    12601 ggcgatttta tcgaaatagt taaaaccgtt tgtttcacaa cctttctcgc ctaataacgg
    12661 actggcttta cacccttgcg gcactactgg taacgagcca aaccaggccg ccacatcgcc
    12721 ctgcactttt ggggttaatg accagttcat ccatttgtag gcgcaaaccg gatgtttcgc
    12781 ttcgctatgt agcatggtgg tgtcagccca accggtaacg ccctccttcg ggaaaacggt
    12841 agcgacaggc tggccttcgg ctttcagggc gttggcctga tagggccatg cactggaagc
    12901 aaccacaccc tcgtttttga aatcgctcat ttgcacggta gtgtcatgcc agtagcgatg
    12961 gatcaaatta tgttgatcgc gcagcacttt cagcaccgcc tgatactgtt cttcggtgag
    13021 ttgatacgga tcactgatgc ccaactgtgg ctgcgtggct ttgacgaaca acgcggcatc
    13081 cgcaatgtag atagggccat cataagcctg tacccggcct ttattgctct tgccgtccgg
    13141 cagattctgc tcaacaaaaa ccacttgcca gctatccggc ggcgtcggga aggttttagt
    13201 gttgtacatc agcaggttcg gcccccattg gtaaggtgtg ccgtaaactt tgccaccaac
    13261 gttaaaccag tcgcctttaa ccacgcgcgg atcgagcgct ttccagttgg gaatcaaggc
    13321 ggtattaatt ggctgcacgc gtttgcccat aatcaaacgc agcgaggcat cgccggatgc
    13381 cgtaaccaga tcgtaacccc ctttggccat cagactgacc atttcatcgg aagtcgcggc
    13441 ggttttaaca ttcaccgcgc agcctgtctc tttttcgaat tgcgttaccc agtcgtactg
    13501 tttatcagtt tgtccgcgtt cgatgtatcc cggccaggcg ataatatcca gtcgcccttc
    13561 cggattctct aaattggtag gcggttcggc ggcgtgagcg gtcattattg tcatgctgag
    13621 cgcacacagg ctgctgcggg caaatgtctt gctcataagg tcttactcct gtcgtaatta
    13681 attgttcagc cgccgtgccg taaaaatatc tccttgttta accatagacg gcggcttgca
    13741 ggcacacctt cggccctgag aaaatttaat caggttgtga gctaccgcgc attatgccgt
    13801 ctgtaaggtt atcttctgga gtatagacaa ggagttagaa tatatgactg ctattcga
//
LOCUS       DAZGJT010000111        13489 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZGJT010000111 DAZGJT010000000
VERSION     DAZGJT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.111"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            270..1379
                     /gene="livK"
                     /locus_tag="ACHKFK_003895"
     CDS             270..1379
                     /gene="livK"
                     /locus_tag="ACHKFK_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO9054135.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINTKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQYIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVAFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPTNQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1427..2353
                     /gene="livH"
                     /locus_tag="ACHKFK_003896"
     CDS             1427..2353
                     /gene="livH"
                     /locus_tag="ACHKFK_003896"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO9054136.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMISSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2350..3627
                     /gene="livM"
                     /locus_tag="ACHKFK_003897"
     CDS             2350..3627
                     /gene="livM"
                     /locus_tag="ACHKFK_003897"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO9054137.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTAKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWSCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3624..4391
                     /gene="livG"
                     /locus_tag="ACHKFK_003898"
     CDS             3624..4391
                     /gene="livG"
                     /locus_tag="ACHKFK_003898"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO9054138.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4393..5106
                     /gene="livF"
                     /locus_tag="ACHKFK_003899"
     CDS             4393..5106
                     /gene="livF"
                     /locus_tag="ACHKFK_003899"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO9054139.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5503..6819
                     /gene="ugpB"
                     /locus_tag="ACHKFK_003900"
     CDS             5503..6819
                     /gene="ugpB"
                     /locus_tag="ACHKFK_003900"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO9054140.1"
                     /translation="MKPLRYTASALALGLALMANAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFRAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADIATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6917..7804
                     /gene="ugpA"
                     /locus_tag="ACHKFK_003901"
     CDS             6917..7804
                     /gene="ugpA"
                     /locus_tag="ACHKFK_003901"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO9054141.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDAFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7801..8646
                     /gene="ugpE"
                     /locus_tag="ACHKFK_003902"
     CDS             7801..8646
                     /gene="ugpE"
                     /locus_tag="ACHKFK_003902"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO9054142.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQEVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLISPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8648..9718
                     /gene="ugpC"
                     /locus_tag="ACHKFK_003903"
     CDS             8648..9718
                     /gene="ugpC"
                     /locus_tag="ACHKFK_003903"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO9054143.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTTGDIWIDRKRVTEMEPKDRGIAMVFQNYALYPHMSVEENMVWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVREPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLAGRVNNEGTHFELDGGITLLLNGGYRQYAGRKM
                     TLGIRPEHIALSSRAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLPENQLHLFDGETGQRV"
     gene            9715..10458
                     /gene="ugpQ"
                     /locus_tag="ACHKFK_003904"
     CDS             9715..10458
                     /gene="ugpQ"
                     /locus_tag="ACHKFK_003904"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO9054144.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGARYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLCVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKTVALAARELWTGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10445..10885)
                     /gene="yhhA"
                     /locus_tag="ACHKFK_003905"
     CDS             complement(10445..10885)
                     /gene="yhhA"
                     /locus_tag="ACHKFK_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO9054145.1"
                     /translation="MKRLLLLTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKEMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            11005..12738
                     /gene="ggt"
                     /locus_tag="ACHKFK_003906"
     CDS             11005..12738
                     /gene="ggt"
                     /locus_tag="ACHKFK_003906"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO9054146.1"
                     /translation="MIKPTFLRRAAIAALLTGSCFSTVAAPPVSYGVEEDVFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYTLAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLIRKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMQKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQVLTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTIKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(12776..13060)
                     /gene="yrhB"
                     /locus_tag="ACHKFK_003907"
     CDS             complement(12776..13060)
                     /gene="yrhB"
                     /locus_tag="ACHKFK_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO9054147.1"
                     /translation="MITYHDAFAKANNYLDDANLPVVITLHGRFSQGWYFCFEAREFF
                     ETGDEAARLAGNAPFIVDKGSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(13260..13415)
                     /locus_tag="ACHKFK_003908"
                     /pseudo
     CDS             complement(13260..13415)
                     /locus_tag="ACHKFK_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3073 a         3364 c         3784 g         3268 t
ORIGIN      
        1 tcccctgaat ggtagtgaat tattctgcca tttgtaccgt tattttatgc tgacaacggc
       61 gcttttttct gtttatctat caataaattc agaatattat ctgttcttaa tcgactgaaa
      121 aatagggatt ttaatcgcta tcatcacaaa atactgcgct aaacccttaa tcagacaggc
      181 aaaaacagtg cagtataaaa aaagaacagt ctgatttgtt aacacataaa aacaaagcaa
      241 cacaacatca cgaatgggga tttttgacta tgaaacggaa tgcgaaaact atcatcgcag
      301 ggatgattgc actggcaatt tcacacaccg ctatggctga cgatattaaa gtcgccgttg
      361 tcggcgcgat gtccggcccg attgcccagt ggggcgatat ggaatttaac ggcgcgcgtc
      421 aggcaattaa agacattaat accaaagggg gaattaaggg cgataaactg gttggcgtgg
      481 aatatgacga cgcctgcgac ccgaaacaag ccgttgcggt cgccaacaaa atcgttaacg
      541 acggcattaa atatgttatt ggtcatctgt gttcctcctc tacccaacct gcatcagata
      601 tctatgaaga cgaaggtatt ctgatgatct cgccgggagc gaccaatccg gagctgaccc
      661 aacgcggtta tcaatacatc atgcgtactg ccgggctgga ctcttcccag gggccaacgg
      721 cggcaaaata cattcttgag acggtgaagc cccagcgcat cgccatcatt cacgacaaac
      781 aacagtatgg cgaagggctg gcacgttcgg tgcaggacgg gctgaaagcg gctaacgcca
      841 acgtcgcctt cttcgacggc attaccgctg gagagaaaga tttctccgcg ctgatcgccc
      901 gcctgaaaaa agaaaacatc gacttcgttt actacggcgg ttactacccg gaaatggggc
      961 agatgctgcg ccaggcccgt tccgttggcc tgaaaaccca gtttatgggg ccggaaggtg
     1021 tcggtaatgc gtcactgtcg aacattgccg gtgatgccgc tgaaggcatg ttggtcacca
     1081 tgccaaaacg ctatgaccag gatccgacaa accagggcat cgttgatgcg ctgaaagccg
     1141 acaagaaaga tccgtccggg ccctatgtct ggatcaccta cgcggcggtg caatctctgg
     1201 caactgccct tgaacgtacc ggcagcgatg agccgctggc gctggtgaaa gatttaaaag
     1261 ctaacggtgc aaacaccgta attgggccgc tgaactggga tgaaaaaggc gatcttaagg
     1321 gattcgattt tggtgtcttc cagtggcacg ccgacggttc atccacggca gccaagtgat
     1381 catcctaccg tccgtgaaaa acgggcgggt ttagaaaggt taccttatgt ctgagcagtt
     1441 tttgtatttc ttgcagcaga tgtttaacgg cgtcacgctg ggcagtacct acgcgctgat
     1501 agccatcggc tacaccatgg tttacggcat tatcggcatg attaacttcg cccacggcga
     1561 ggtttacatg attagtagct atgtctcatt tatgatcatc gccgcactga tgatgatggg
     1621 tattgatacc ggctggctgc tggtggcggc gggattcgtc ggcgcaatcg tcattgccag
     1681 cgcctacggc tggagtatcg aacgggtagc ctatcgcccg gtgcgtaact ctaagcgcct
     1741 gattgcgctt atttccgcaa tcggcatgtc tatcttcctg caaaactacg tcagcctgac
     1801 cgaaggttcg cgcgacgtgg cgctgcccag cctgtttaac ggtcagtggg tagtgggtca
     1861 tagcgaaaac ttctctgcct ctatcactac catgcaggcg gttatctgga ttgttacttt
     1921 cctcgccatg ctggcactga cgattttcat tcgctattcc cgtatggggc gagcctgccg
     1981 tgcctgcgcg gaagatctga aaatggcgag cctgcttggc atcaacaccg accgggtgat
     2041 tgcgctgacc tttgtgattg gcgcggcgat ggcggcagtg gcgggcgtgc tgctcggtca
     2101 gttctacggc gtaattaacc cctacatcgg ctttatggcc gggatgaaag cctttaccgc
     2161 tgcggtgctc ggtgggattg gcagcattcc gggggcgatg attggcgggc tgatcctggg
     2221 gattgcggag gcgctctctt ctgcctatct gagtacggaa tataaagatg tggtctcatt
     2281 cgctctgctg atcctggtgc tgctggtgat gccgaccggt attctgggtc gcccggaggt
     2341 agagaaagta tgaaaccgat gcatattgca atggcgctgc tctctgccgc tatgttcttt
     2401 gtgctggcgg gcgtctttat gggcgtgcaa ctggagctgg atggcaccaa actggtggtc
     2461 gacacggctt cagacatccg ttggcagtgg gtgtttatcg gcactgcggt ggtctttttc
     2521 ttccagcttt tgcgacctgc tttccagaaa gggctaaaaa gcgtttccgg gccgaagttt
     2581 attctgcccg cgattgatgg ttccacggcg aagcagaaac tgttcctcgt ggcgctgctg
     2641 gtgcttgcgg tggcgtggcc gtttatggtg tcgcgtggga cggtggatat cgccactctg
     2701 accatgatct acattattct cggtctcggg ctgaacgtgg ttgttggtct ttctggtctg
     2761 ctggtgctgg gatacggcgg cttttatgcc atcggcgctt acacttttgc gctgctcaat
     2821 cactattacg gtctgggctt ctggagctgt ctgccgattg ccgggttgat ggcggcggcg
     2881 gcgggcttcc tgctcggctt cccggtgttg cgtctgcgcg gtgactatct ggcgatcgtt
     2941 accctcggtt tcggcgaaat tgtgcgcata ttgctgctca ataacaccga aattaccggc
     3001 ggaccgaacg gtatcagtca gattccgaaa ccgacgctct tcggactcga gttcagccgt
     3061 accgcccgtg aaggcggctg ggacacgttc agtaatttct ttggcctgaa atacgatccc
     3121 tccgatcgcg tcatcttcct ctacctggtg gcgttgctgc tggtggtgtt aagcctgttt
     3181 gtcattaacc gcctgctgcg gatgccgctg gggcgtgcgt gggaagcgtt gcgtgaagat
     3241 gaaatcgctt gtcgttcgct gggcttaagc ccgcgtcgta tcaagctgac tgcctttacc
     3301 atcagcgctg cgtttgccgg ttttgctgga acgttgtttg ctgcgcgcca gggctttgtc
     3361 agccctgaat ccttcacttt tgccgaatcg gcgtttgtgc tggcgatagt ggtgctcggc
     3421 ggtatgggct cgcaatttgc agtgattctg gcggctgttt tgctggtggt atcgcgcgag
     3481 ttgatgcgtg atttcaacga atacagcatg ttaatgctcg gtggtttgat ggtgctgatg
     3541 atgatctggc gtccgcaggg cttgctgccc atgacgcgcc cgcaactcaa gctgaaaaac
     3601 ggcgcagcaa aaggagagca ggcatgagtc agccattatt atctgttaac ggcctgatga
     3661 tgcgcttcgg cggcctgctg gcggtgaaca acgtcaatct tgaactgtac ccgcaggaga
     3721 tcgtctcgtt aatcggccct aacggtgccg gaaaaaccac ggtcttcaac tgcctgaccg
     3781 gattctataa acccaccggc ggcaccattt tactgcgcga tcagcatctg gaaggtttac
     3841 cggggcagca aattgcccgc atgggcgtgg tgcgcacctt ccagcatgta cgtctgttcc
     3901 gtgaaatgac ggtgattgaa aacctgctgg tggcacagca tcagcaactg aaaactgggc
     3961 tgttctccgg cctgttgaaa accccctctt tccgtcgcgc ccagagcgaa gcgctcgacc
     4021 gcgccgctac ctggcttgag cgcattggtt tgctggaaca tgccaaccgt caggcgagta
     4081 acctggccta tggtgaccag cgtcgtctgg agattgcccg ctgtatggta acgcagccgg
     4141 agattttaat gctcgacgaa ccggcagcag gtcttaaccc gaaagagacg aaagagctgg
     4201 atgagctgat tgccgaactg cgtaaccatc acaacaccac tatcttgttg attgaacacg
     4261 atatgaagct ggtgatgggg atttcagaca gaatttacgt ggtcaatcag gggacgccgc
     4321 tggcaaacgg tacaccggag cagatccgta ataacccgga cgtgatccgt gcctatttag
     4381 gtgaggcata agatggaaaa agtcatgttg tcctttgaca aagtcagcgc ccactacggc
     4441 aaaatccagg cgctgcatga ggtgagcctg catatcaatc agggcgagat tgtcaccctc
     4501 attggcgcga acggggcggg gaaaaccacc ttgctcggca cgttatgcgg cgatccgcgc
     4561 gccaccagcg ggcgaattgt gtttgatgat aaagacatta ccgactggca gacggcgaaa
     4621 atcatgcgcg aagcggtagc gattgtcccg gaaggacgtc gcgtcttctc gcggatgacg
     4681 gtggaagaga acctggcgat gggcggcttt tttgccgaac gcgaccagtt ccaggagcgc
     4741 ataaagtggg tctatgaact gtttccacgt ctgcatgagc gccgtgttca gcgagcgggc
     4801 actatgtctg gcggtgaaca gcagatgctg gcgattggtc gcgcgctgat gagcaacccg
     4861 cgtttgttgt tgcttgatga gccatcgctc gggctggcac cgattatcat ccagcaaatt
     4921 ttcgacacca tcgaacaact gcgcgagcag gggatgacca tcttccttgt cgagcagaac
     4981 gccaaccagg cgctaaagct ggcggatcgc ggctacgtgc tggaaaacgg tcatgtagtg
     5041 ctctccgata ccggcgacgc gctgctggcg aatgaagcgg tgagaagtgc gtatttaggc
     5101 gggtaataac acgttgattg ataaggagtc aaaagactcc tttgagacag gtgacaaatg
     5161 taaaattgcc tgatgtgctg cgcttatcag gcctactggg tgagttgcaa tatattgaat
     5221 ttgcacgatc ttgtaggctg gataaggcgt ttacgccgca tccggcatga aacgatgagt
     5281 aatctgtaga gtttgattca gaccttctat ttttccgctt atccgtgccc catctcccat
     5341 tttccctcgc ccgcgccgtc accgccttgt catctttctg acaccttact atcttaaaaa
     5401 tgtaacaaaa tagttatttt tctgtaattc gagcatgtca tgttactccg cgagcataaa
     5461 acgcgtgaat tcgcgcattc ggtacaacaa gagagataat caatgaaacc gttacgttat
     5521 acagcttcag cactggcgct cggactggct ttaatggcaa atgcgcaggc agtgacgacc
     5581 attccgttct ggcattctat ggaaggggaa ctgggtaaag aggtggattc tctggcccaa
     5641 cgttttaacg ccgaaaaccc ggattacaaa attgtaccga cctataaagg caactacgaa
     5701 cagaatttaa gcgcggggat tgccgcattt cgtaccggca acgctccggc tattttgcag
     5761 gtttatgaag ttggcaccgc caccatgatg gcgtcgaaag ccattaaacc ggtgtatgac
     5821 gtatttaaag aggcagggat tcaattcgat gagtcgcagt ttgtgccgac ggtttcaggc
     5881 tattactccg acagcaaaac cggccactta ctctcccagc cgttcaacag ctcgactccc
     5941 gttctctatt acaacaaaga cgccttcaag aaagcaggat tagacccgga acagccgccg
     6001 aaaacctggc aggatctggc ggactatgcc gcaaaactga aagcctccgg tatgaagtgc
     6061 ggctacgcca gcggctggca gggatggatc caactggaaa actttagagc ctggaacggt
     6121 ttgccgtttg ccagcaaaaa caacggcttt gacggcacag acgcggtgct tgagttcaac
     6181 aagccggagc aggtgaaaca catcgccatg ctcgaagaga tgaacaagaa gggcgatttc
     6241 agctacgttg ggcgtaagga tgaatccacc gagaagttct ataacggtga ttgcgcgatg
     6301 acgaccgcct cttccggttc tcttgccaac attcgcgagt acgccaaatt taactatggc
     6361 gtaggcatga tgccttacga cgccgatgcg aaagacgcgc cgcaaaacgc cattatcggc
     6421 ggagccagtc tatgggtaat gcagggtaaa gataaagaaa cctacaccgg cgtggcgaag
     6481 ttcctcgact tcctcgcaaa gccagaaaac gctgccgagt ggcatcagaa aaccggctat
     6541 ctgccaatca ccaaagcggc gtatgacctg acccgtgagc agggctttta cgagaaaaac
     6601 ccaggagcgg atattgccac gcgtcagatg ctgaacaagc caccgttgcc gttcaccaaa
     6661 gggctgcgtc tgggcaacat gccgcagatc cgcgtgattg tggatgaaga gctggagagc
     6721 gtgtggaccg gtaagaagac accacagcag gcgctggata ctgccgttga gcgtgggaac
     6781 cagttactgc gccgctttga gaaatcgacg aagtcttaat cagtgtaatg tcggatgcgt
     6841 ttcgcttatc tgacctggca tcgcgtgtag gccggataag cgaagcgcat ccggcacagt
     6901 tcaggaatta actgtaatgt catcatcccg tccggtgttc cgctcgcgct ggctgcctta
     6961 tttgctggtc gcgccgcagc tcatcatcac cgttatcttt tttatctggc ctgcgggcga
     7021 agcgttgtgg tactcgctac aaagcgtcga tccgtttggt ttctccagcc agtttgtcgg
     7081 cctggataat ttcgtcacgc tgtttcacga cagctactat ctcgacgcct tctggacgac
     7141 gataaaattc agcacctttg tcaccgtcag cggtttgctg gtatcgctgt tcttcgcagc
     7201 gctggtggag tacatcgtgc gcggcagtcg tttctatcaa accttgatgc tgctgccgta
     7261 tgccgtggct cccgccgttg ccgccgtatt gtggatcttt ctgtttaacc ccggtcgcgg
     7321 gctgatcacc cattttctcg ctgagttcgg ctacgactgg aaccacgcgc aaaacagcgg
     7381 tcaggcgatg ttcctggtgg tgtttgcctc agtgtggaag caaatcagct acaactttct
     7441 gttcttctat gccgcgctgc aatccatccc ccgttcgttg atcgaagccg cagccattga
     7501 tggggctggc ccgattcgcc gcttctttaa gattgcgctg ccgcttatcg ctccggtgag
     7561 tttcttcctg ctggtggtga acctggtgta tgccttcttc gacaccttcc cggtgatcga
     7621 cgccgccacg tccggcgggc cagtacaggc caccacgacg ctgatttata agatttaccg
     7681 tgaaggcttt accggactgg atttggcttc gtctgccgcg cagtcggtgg tgttgatgtt
     7741 cctcgtcatc gtgctgacgg tggtgcagtt ccgctatgtt gaaagcaagg tacgttacca
     7801 atgattgaga accgtccgtg gctgacaata ttcagccata ccatgctgat cctcgggatc
     7861 gcggtgatcc tcttcccgct gtacgtggcg tttgtcgcgg cgacgctgga taaacaagaa
     7921 gtctatgccg caccgatgac gctaatcccc ggcacacatc tgctggaaaa catccacaac
     7981 atctgggtaa acggggtagg cacgaatagc gcgccgttct ggcggatgtt gcttaacagc
     8041 tttgtgatgg cgttcagcat tacgctcggc aaaattaccg tctcgatgct ctcggcattt
     8101 gccattgtct ggtttcgttt tccgctacgt aacctcttct tctggatgat ttttatcacc
     8161 ctgatgctgc cggttgaagt gcgtatcttc ccgacggtgg aagtcatcgc caacctgaaa
     8221 atgctcgaca gctacgcagg tttaacgctg ccgctgatgg cctcggcgac cgccacgttt
     8281 ctgtttcgcc agttctttat gaccctaccg gatgaactgg tggaagccgc gcggatcgac
     8341 ggcgcgtcgc caatgcgttt cttttgcgac atcgtttttc cgctgtccaa aaccaatctg
     8401 gcggcgctgt ttgtgattac ctttatttac ggctggaacc agtatttgtg gccgttgttg
     8461 attattaccg acgttgatct cggcaccacc gtggcaggga ttaaagggat gatcgccaca
     8521 ggcgaaggca caacggaatg gaactcggtg atggcggcga tgttgttaac cctgatctca
     8581 ccagtagtga ttgttttagt gatgcagcgt gccttcgtgc gcggcctggt cgatagtgag
     8641 aaataagatg gcaggactga aattacaggc agtaaccaaa agctgggatg gcaaaaccca
     8701 ggtcattaaa ccgctgaccc ttgatgtggc ggatggcgaa tttatcgtga tggtcgggcc
     8761 gtctggctgc gggaaatcga cgttattgcg aatggttgcc ggactggagc gggtaacgac
     8821 gggcgatatc tggatcgacc gcaagcgtgt caccgaaatg gagccgaaag atcgcgggat
     8881 tgcgatggtg ttccagaact acgcgctcta tccgcatatg agcgtagagg agaacatggt
     8941 gtgggggctg aaaattcgcg gcatgggcaa gcaacaaatt gccgaacgtg ttaaagaagc
     9001 ggcgcgcatt ctggagctgg acggcctgct taagcgccgc ccgcgtgagc tatccggcgg
     9061 tcagcgtcag cgtgtggcga tggggcgtgc gattgtgcgt gagccggcag tattcctgtt
     9121 tgatgagccg ctctctaacc tcgatgccaa gttgcgtgtg cagatgcgtc ttgaactgca
     9181 acagctgcac cgtcgcctga aaacgacttc actctacgtt acccacgatc aggttgaggc
     9241 gatgacgctc gcccagcgag taatggtgat gaacggcggc gtcgccgaac agattggcac
     9301 gccagttgaa gtgtacgaaa agcccgccag cctgtttgtg gcgagtttta tcggcagccc
     9361 ggcgatgaac ctactggctg gccgcgtgaa taacgaaggt acgcatttcg aactggatgg
     9421 tggtattacg ctgctgctaa acggtggcta ccgtcagtat gccgggcgta aaatgaccct
     9481 cggcattcgc ccggaacata tagcgctaag ctcgcgggca gaaggtggtg tgccgctggt
     9541 gatggacacg ctggagatcc tcggcgcaga taacctggcg cacggacgct ggggcgaaca
     9601 gaagctggtg gtgcgactgg cgcatcagga gcgcccgacg gcaggcagca cgctgtggct
     9661 gcatctgccg gaaaatcagc tgcatctttt tgatggtgaa acaggacaac gagtatgagt
     9721 aactggcctt atccccgcat cgtcgcccat cgtggtggcg gtaagctggc cccggaaaac
     9781 actctggcgg caatcgacgt cggggcaaga tacggtcata agatgatcga atttgacgca
     9841 aagttatcga aagatggcga gatcttcctg ctccatgacg acaatctcga acgtaccagc
     9901 aacggttggg gcgttgcggg cgagctgaac tggcaggatt tactgtgcgt ggatgcaggc
     9961 agttggtaca gcaaagcgtt taaaggtgag ccgctgccgt tgctttcgca ggtggcggaa
    10021 cgctgccgcg aacacgggat gatggcgaat atcgaaatca aacccaccac tggcactgga
    10081 ccattaacgg gcaaaacggt ggcgttggcg gcacgcgaac tgtggaccgg tatgacgccg
    10141 ccgctgctgt catcgtttga gattgatgct ttagaagccg cgcaacaggc agcaccggaa
    10201 ctgccgcgtg gtttattgct tgatgaatgg cgcgacgact ggcgagaatt gaccgcgcga
    10261 ctgggctgcg tctctattca tctcaatcat aagttgctcg ataaagcgcg agtgatgcag
    10321 ttgaaagatg ccggattacg gattctggtt tataccgtca ataaacccca gcgcgcggca
    10381 gagttgctgc gctggggcgt ggattgcatc tgtaccgatg cgattgacgt gattggtccg
    10441 aactttacgg cccaatagtt ttcaacggaa tgtcaggctg cggcgtgctg ggctgattca
    10501 gcatgtcgcc gtttctcctc tccggcagca tatgctgctg attcaacgaa ctatccggat
    10561 tacggttgct gtttaacatc ccgccgttgg tgttgggcag catttgctgc cgggcgggat
    10621 ttcgctcccc cggctgtgac tgcaacaccc gctgagaatt attgtttatc tggttttcta
    10681 aatgctgctg ttgcagctgc gtttgcgttt tcagttgctg gttcagcatc tctttttgct
    10741 ggatttgctg cgtctgcatt tgagtttgca tccgctgctg gctggggatc tgatatcccg
    10801 gctggttagg gttgttcaga gtattaatgg gctgtgcaaa gccgacaaac ggcaggagtg
    10861 ccgtcaaaag cagaagtcgt ttcatcgcgt atcctcctct gaagatattc tttaagttta
    10921 ctcgcttccc ggcaaaacga tgattaattc agagttatat accaggctta gctggggttg
    10981 ccccttaatc tctggagaat aacgatgata aaaccgacgt ttttacgccg ggcggccatt
    11041 gctgctctgc tcacaggaag ttgttttagt acggtagccg cgccacctgt ttcttatggt
    11101 gtggaggaag atgtctttca tccggtacgc gctaagcagg gaatggtggc gtctgtagat
    11161 gctactgcca ctcaggtggg ggtggatatc ctcaaggagg gcgggaatgc cgttgatgcc
    11221 gccgtggcgg tgggctacac gctggcagta acgcatccgc aggcagggaa tctgggcggc
    11281 ggtggtttta tgctgatccg ctcgaaaaat ggcaatacca cggctatcga tttccgcgaa
    11341 atggcacccg ccaaagcgac ccgcgatatg ttcctcgatg atcagggcaa cccggacagc
    11401 aaaaaatcac tcacttcgca tctggcttcc ggcacacccg gtacggtagc gggtttctcg
    11461 ttggcgctgg ataaatacgg caccatgccg ttgaataaag tggtgcaacc ggcgtttaaa
    11521 ctggcacgcg atggttttat cgttaacgac gcgctggctg acgatctcaa aacctacggc
    11581 agtgaagtgc tgccgaacca cgaaaacagc aaagctatct tctggaaaga gggcgaaccg
    11641 ctgaaaaagg gcgacaagct ggtgcaggcg aacctggcaa agagcctgga gatgattgcc
    11701 gaaaacggcc cggatgaatt ctacaaaggc acgatagcgg aacagattgc ccaggagatg
    11761 cagaaaaacg gtggcttgat cagaaaagaa gatttagcgg catataaagc ggtcgaacgc
    11821 acaccgataa gcggcgatta tcgcgggtat caggtttact ccatgccacc gccatcttcc
    11881 ggcggtattc atatcgtaca aatcctcaat attctggaaa acttcgatat gcagaagtac
    11941 ggctttggca gcgccgacgc gatgcaaatc atggcagaag ctgagaaata cgcctatgcc
    12001 gaccgctcgg aatatcttgg cgacccggat tttgtcaaag taccgtggca ggtgctgacc
    12061 aataaagcct atgccaaatc tattgccgat caaatcgata tcaataaagc gaagccatcc
    12121 agcgaaattc gtcctggcaa gcttgcgcct tatgagagta atcaaactac ccattactca
    12181 gtagtggata aagacggtaa cgccgtggcg gtgacctata cgctgaacac caccttcggt
    12241 acgggcattg tcgcgggcga gagcggtatt ttgctcaata accagatgga tgatttctcc
    12301 gccaaaccag gcgtaccgaa cgtttacggg ctggtgggcg gcgatgccaa cgccgtcggg
    12361 ccgaataaac gccctctgtc gtcgatgtca ccaaccattg tggtcaagga tggtaaaacc
    12421 tggctggtca ccggaagccc aggcggtagc cggattatca ccactgtgct gcaaatggtg
    12481 gtgaatagca tcgattatgg aatgaacgtc gccgaagcga ccaatgcgcc acgtttccac
    12541 catcagtggt tgccggacga gctgcgtgtc gaaaaagggt ttagcccgga tacgatcaag
    12601 ctgctggaag caaaaggtca gaaagtggcg ctgaaagagg cgatgggcag tacacaaagc
    12661 attatggttg ggccggacgg tgagctgtac ggtgcatccg acccgcgctc ggtggatgat
    12721 ttaacggcgg ggtattaagg ttagcggccc tcttcgtggg aagagggccg tattgtcagg
    12781 gcaagccgaa ggtagccttt tttatttcat aatcctgtag atattcttcc agcggctttg
    12841 ccgttccaag ggaatgaatt tcaccactgc ctttatcaac aataaaaggt gcgttaccag
    12901 ctaagcgcgc ggcctcatct ccagtttcga aaaattctcg tgcttcgaaa cagaaatacc
    12961 agccctggct aaagcgtcca tgtagagtaa tgacgaccgg gagatttgca tcatcaaggt
    13021 aattgttcgc tttcgcgaat gcgtcgtgat aagtaatcat aattataata aatattattg
    13081 tttagcgtta tattattatt cctgcgttat acattgagca aagacgcgtt catcttcgtc
    13141 aaagatatta ttaaatgctg gttttgaaaa gagttcatcg gataaggtat agtcacaaaa
    13201 taagtatctg ctaatatttt gtgatttatt ttccagaacg tcgaaccgga tttgctcatt
    13261 taagatatca gaatcttatg attcgggtaa aattctaacc aaatcaacat gtcactaaga
    13321 tcatcatcgc cattagctat ttcagtttaa atagcgacta catgtcttcg gtccatctca
    13381 cttaaggagt gtagttccgt tgtaagtttt tctatagctt gtattgataa atttcgatca
    13441 agaaatttac tgctggtaaa ctctaaaaag atggcatggt ttacaatga
//
LOCUS       DAZGJT010000112        13469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZGJT010000112 DAZGJT010000000
VERSION     DAZGJT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.112"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            218..1432
                     /gene="manD"
                     /locus_tag="ACHKFK_003909"
     CDS             218..1432
                     /gene="manD"
                     /locus_tag="ACHKFK_003909"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO9054148.1"
                     /translation="MKIIAVDVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLADEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGRSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFDEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1499..2782
                     /locus_tag="ACHKFK_003910"
     CDS             1499..2782
                     /locus_tag="ACHKFK_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9054149.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTEEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            3034..3951
                     /gene="oxyR"
                     /locus_tag="ACHKFK_003911"
     CDS             3034..3951
                     /gene="oxyR"
                     /locus_tag="ACHKFK_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO9054150.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3934..5334)
                     /gene="sthA"
                     /locus_tag="ACHKFK_003912"
     CDS             complement(3934..5334)
                     /gene="sthA"
                     /locus_tag="ACHKFK_003912"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO9054151.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEANAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5664..6830
                     /locus_tag="ACHKFK_003913"
     CDS             5664..6830
                     /locus_tag="ACHKFK_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 aminoacylase family protein"
                     /protein_id="HFO9054152.1"
                     /translation="MIEHYIRGFEEDLREIRHQIHENPELGLQEFKTSALVAEKLRQW
                     GYEVEQGLATTGVVATLKVGDGEKSIGLRADMDALPIYENSGKPWASKHPGLMHACGH
                     DGHTTILLGAARYFAETRRFSGTLRLIFQPAEEMINGGEIMVKEGLFDRFPCDVIFGM
                     HNMPGLPVGKFFFQPGALMASMDQFHITVRGCGGHGAIPHKAIDPVLVAAHITTALQS
                     IVSRNVDPLEAAVITVGSIVAGEAANVIPDSAEMKISVRSLSRDTRQLLLTRIPALAQ
                     AQAASFGATAEVTHVNGTPVLVNDEEMARFAWQVACKTFGEDRAEFGIKPLMGSEDFS
                     FMLEAQPKGGFLLFGNGDVGEGACMVHNPGYDFNDASLVPASSYWGALVEAWLQ"
     gene            6873..8189
                     /locus_tag="ACHKFK_003914"
     CDS             6873..8189
                     /locus_tag="ACHKFK_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate-proton symporter"
                     /protein_id="HFO9054153.1"
                     /translation="MTAIVNETVRVKKKPSGRRVIFASAFGNALEFFDFGVYNFFVVY
                     ISTLFFPPSADHNVALLLAFATFGVSFFMRPLGGIIVGAWADRFGRKPAMVFTIALMS
                     LGTLMIGIAPTYETAGYWGTATLVLARLIQGVAAGGEVGASMSLLVESAPANRRGFYS
                     SWSLATQGLATTFGGVVALGLSAWLPFATGSETVMAEWGWRVPFFIGVLLAPIGCWLR
                     LSLENDVPEPVRNKKAATSESAFSLLLQHKATIVNGVLLAIGSTVATYISLFYYGTWA
                     AKYLAMPQHYSHAAMLLAGVVTFVGALLVGMLCDSVGRKKLILISRVMVLLCSWPSFW
                     LLVNYPSPGMLLTVVFVMVSFTTLGGVPVMLLISELLPKRIRALGFALVYSIGVAIFG
                     GFAQYFATQSIVLLDSLTAPAWYLGGGTLLSMLALLYVKEPAKELQ"
     gene            8293..8943
                     /gene="fabR"
                     /locus_tag="ACHKFK_003915"
     CDS             8293..8943
                     /gene="fabR"
                     /locus_tag="ACHKFK_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO9054154.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            8943..9302
                     /gene="yijD"
                     /locus_tag="ACHKFK_003916"
     CDS             8943..9302
                     /gene="yijD"
                     /locus_tag="ACHKFK_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO9054155.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(9342..10442)
                     /gene="trmA"
                     /locus_tag="ACHKFK_003917"
     CDS             complement(9342..10442)
                     /gene="trmA"
                     /locus_tag="ACHKFK_003917"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO9054156.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPILR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQQAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPIAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      10556..10746
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10811..12655
                     /gene="btuB"
                     /locus_tag="ACHKFK_003918"
     CDS             10811..12655
                     /gene="btuB"
                     /locus_tag="ACHKFK_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO9054157.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDHPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQPDNDGFL
                     SKTLYGALEHNFTDVWSGFVRGYGYDNRTNYDAYYSPGLPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNIIIGHGNIGAG
                     VDWQKQSTAPGTAYVEDGYDQRNTGIYLTGLQQVGDFTFEGAGRSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGSYGNPNLNPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            12600..13457
                     /gene="murI"
                     /locus_tag="ACHKFK_003919"
     CDS             12600..13457
                     /gene="murI"
                     /locus_tag="ACHKFK_003919"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9054158.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEVFIVERVVEIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLD"
BASE COUNT         3046 a         3122 c         3659 g         3642 t
ORIGIN      
        1 tttaatgatt aatggagttt gttatgtgat tatttaacgt gttgttttaa cgatgatggc
       61 taattattca ggtggttttt gagggtgttt tttctcctcg gtagaaattt ttatttttaa
      121 tgtgatcacg gttgaataat cactttttct ctcttgtatg gtagtaggcg taaaattaag
      181 tttgaagtgt ctatttacaa tacaggagat tgcaataatg aaaattattg ctgtggatgt
      241 ttttgttact tgtcccgggc gtaatttcgt tacgcttaaa ataaccactg aaagcggact
      301 ttgtggatta ggagatgcca cgttaaatgg tcgcgaattg tcggtcgctt cttatctgaa
      361 agatcatctt tgtcctcaat taattggccg cgatgccagc cgtatcgaag atatctggca
      421 attcttttat aaaggagctt actggcgacg tggtcccgtc acgatgtcgg ccatttctgc
      481 cattgatatg gcgttgtggg atatcaaagc taaagcggca aatatgccat tgtatcaact
      541 gcttggtggc gcttctcgtg aaggcgtgat ggtttattgc cacaccaccg ggcggacgat
      601 tgatgaagtc cttgaagact atgcaaagca ccagcagatg ggctttaagg cgattcgtgt
      661 gcagtgtggt gtaccgggta tgcaaaccac ctatggtctg gcgaagggca aaggactggc
      721 ggatgaacca gcaaccaaag gcctgtggcc agaagagcag ttgtggtctt cagaaaagta
      781 tctcgatttt acgccgaagt tatttgaagc ggtgcgtaat aagttcggtt ttaacgaaca
      841 tctgttgcat gacatgcacc atcgcctgac ccccatcgaa gcggcgcgct ttgggaggag
      901 cattgaggat taccgcctgt tctggatgga agatccgacg cctgccgaga accaggaatg
      961 ttttcgtctg attcgccagc ataccgtgac gccgattgcg gtcggtgaag tgtttaacag
     1021 tatctgggac tgcaaacagt taatcgaaga gcagctgatt gactatattc gcacgacgat
     1081 tacccatgca ggcggcatta ccgggatgcg acggattgcc gattttgcat cgctttatca
     1141 ggtacgtacc ggttcgcatg gcccttcaga tttatcgcct gtttgtcatg cggcggcgct
     1201 gcattttgac ctctgggtgc caaactttgg cgttcaggag tacatgggct attccgaaca
     1261 aatgcttgaa gtcttcccgc acagctggcg cttcgatgaa ggttatatgc acccaggcga
     1321 tgaaccgggg ctgggtattt ctttcgatga gaaactggcg gcgaaatatc cgtatgaccc
     1381 ggcgtatctc cccgtcgcac gactggaaga tggcacattg tggaactggt aatagcggtt
     1441 cgcttaatta ttttctgaat attcatcaaa tcgattaatt ccgctcaagg agctaaaaat
     1501 ggacgttgat gtgtcaacca gtgttgcggg taataaaccg caacggattc gtcgtattca
     1561 gacagtgacc ctggttttat tatttatggc ggggatcgtt aattttctcg accgttcatc
     1621 attgagcgtg gcaggtgaag cgattcgtgg cgagttagga ttatcggcga cagagtttgg
     1681 tgttttgctt tctgcatttt ctctgtctta tggtttttca caacttcctt ccggtatttt
     1741 gctagaccgc tttggcccac gaatagtgtt aggcgcaggc ttaatattct ggtcattaat
     1801 gcaggcatta acaggaatgg ttaattcttt tagccacttt attttaatgc gtatcggtct
     1861 gggtattggc gaagcgccat ttatgcctgc gggagtaaag tcgatcaccg actggtatgc
     1921 gcaaaaagaa cgcggcacgg cgctggggat ttttaactca tctaccgtta tcggtcaggc
     1981 tatcgcgcct cctgctcttg tattgatgca actggcatgg ggctggcgga cgatgtttgt
     2041 catcatcggc gtagcaggga ttctggttgg gatctgttgg tacgcgtggt atcgcaaccg
     2101 ggcgcagttt gtcctgaccg aggaagaacg gacatatctt tccgcgccgg ttaaaccgcg
     2161 tccacagtta caatttagcg agtggctggc gctgtttaag catcggacaa cctgggggat
     2221 gattttaggt ttctctggcg tcaattatac cggttggctc tacatcgcgt ggttacccgg
     2281 ttatttgcag gccgaacaag gtttcagtct ggcgaaaacc ggttgggtag cggcaattcc
     2341 tttcctcgcg gcggcggtcg ggatgtgggt taacggtatt gttgtcgatc ggctggcgaa
     2401 aaaaggctat gacctggcga agacgcgtaa aacggcgatt gtctgcggtt tgatgatgtc
     2461 ggcattaggc acgttgctgg tcgtgcaatc ttcctcgcca gcccaggcgg tagcgtttat
     2521 ctcaatggcg ctgttctgcg tgcatttcgc gggaacatct gcatgggggc tggtgcaggt
     2581 gatggtgtca gaaacaaaag tggcttccat cgccgggatt caaaactttg gcagttttgt
     2641 ttttgcttcc tttgctccga tcgtaaccgg ttgggtcgtg gataccacac actcgtttaa
     2701 tctggcgctg gttatcgcgg cctgtgtgac gttcaccggg gcgctgtgtt acttctttat
     2761 tgtcaaagat cggattgagt aaccgttact atcaggctct cttgctgtgg gcctgtagaa
     2821 taaaaaaaag cggatcctgg agatccgcaa aagttcacgt tggctttagt tattcgagtt
     2881 gagaaactct cgaaacgggc agtgacttca agggttaaaa gaggtgccgc tccgtttctg
     2941 tgagcaatta tcagtcagaa tgcttgatag ggataatcgt tcgttgctat gctacctatc
     3001 gccgcgaact atcgtggcaa tggaggatgg ataatgaata ttcgtgatct tgagtacctg
     3061 gtggcattgg ccgaacaccg ccattttcgg cgtgcggcag attcctgcca cgttagccag
     3121 ccgacgctta gcgggcaaat tcgtaagctg gaagatgagt tgggcgtgat gttgctggag
     3181 cggaccagcc gtaaagtgtt gttcacccag gcgggaatgc tgctggtgga tcaggcgcgt
     3241 accgtactgc gtgaggtgaa agtccttaaa gagatggcaa gccagcaggg cgagacgatg
     3301 tccgggccgc tgcatattgg tttgattccc acggttggac cgtacctgct accgcatatt
     3361 atcccgatgc tgcaccagac ctttccaaag ctggaaatgt atctgcatga agcacagacc
     3421 caccagttac tggcgcaact ggacagcggc aaactcgatt gcgtgatcct cgcgctggtg
     3481 aaagagagcg aagcattcat tgaagtgccg ttgtttgatg agccaatgtt gctggctatc
     3541 tatgaagacc acccgtgggc gaaccgcgaa tgcgtaccga tggccgatct ggcaggggaa
     3601 aaactgctga tgctggaaga tggtcactgt ttgcgcgatc aggcaatggg tttctgtttt
     3661 gaagccgggg cggatgaaga tacacacttc cgcgcgacca gcctggaaac tctgcgcaac
     3721 atggtggcgg caggtagcgg gatcacttta ctgcctgcgc tggctgtgcc gccggaacgc
     3781 aaacgcgatg gggttgttta tctgccgtgc attaagccgg aaccacgccg cactattggc
     3841 ctggtttatc gtcctggctc accgctgcgc agccgctatg agcagctggc agaggccatc
     3901 cgcgcaagaa tggatggcca tttcgataaa gtgttaaaac aggcggttta agccatttag
     3961 cgcagctacc cgataggctt ccgccatcgt cgggtagtta aaggtggtgt tgacgaagta
     4021 ctcaatagtg ttgccgccac ctttctgttc cataatcgcc tgaccgatat gaataatttc
     4081 ggcagcgcgc tcgccaaagc agtgaatacc cagaatctct tttgtttccc gatggaacaa
     4141 aattttcagc gtgcccacgt tcatgccgac gatttgtgca cgtgccagat gtttaaactg
     4201 ggcgcggccc acttcatatg gcactttcat cgcggtcagc tgctgttcgg ttttgcctac
     4261 agagctgatt tccgggatgg tgtaaatgcc ggtagggata tcttcaatca gatgtgcgtt
     4321 ggcttcacct ttcaccagcg cctgcgcggc aatgcgcccc tggtcatagg ctgccgacgc
     4381 caggctcgga taaccaatca cgtcgcccac cgcgtaaacg tgcggctgtg cggtctgata
     4441 catgctgttg accttcagct gtccgcgact gtcagtttcc agcccaatgt tctgtaacgc
     4501 cagcgaatcg gtattaccgg tgcgaccgtt ggcatagagc agacagtcag ctttcagttt
     4561 tttacccgac ttcagatgca tgatcacacc gtcgtcacag ccttcgatct tctcgtactc
     4621 ttcgttgtga cgaatcacta cgccactgtt ccagaagtga taggagagag aatctgacat
     4681 ctcttgatcg agaaacgcca gcagacgatc gcgggtgttg atcagatcca cttttacatc
     4741 cataccgcgg aagatcgacg catattcaca gccgatcact ccagcaccgt aaataagtac
     4801 atggcgcggt tcgtggtgca tgctaagaat tgagtcgctg tcataaatgc gtggatgggt
     4861 gaaatcaaca tctgttggat gatatggacg agaaccgcag gcaataacaa atttttcagc
     4921 agttagtgtt tcgacgctgc cgtccgggca atccagcgcc aacgtatgct catcaacaaa
     4981 gcgagcgttc ccctgcaata tttcacagtg attacgttcg taaaatccct gacgcatgcg
     5041 cgtttgttga ttaatcacgt tatcggcatg gttaaggata tcggcaaaag aagagcggag
     5101 cagtcgggaa tggtcgctgt atagtgggtt ttgattgaat tctataatgc ggctgacggc
     5161 gtgacggaga gctttcgacg ggatggtgcc ccagtgggtg caaccgccgc caacattttg
     5221 ataacgctcg ataactgcga cgcgcgcacc ttgcttaacc aggcccatcg cagcgccttc
     5281 gccgccgggg ccggaaccta ttactatggc atcgtaatcg taggaatgtg gcatggtagg
     5341 gcttacctgt tcttatacat aaaagcaaca gaatggtaac attttatcgc gggtaagcca
     5401 attgatcccc gtcatttatc tggctatatc gtgagcggcc tttgctttgt cttcctcctt
     5461 cttattttat ttattcacta ttaatcgctt tttcttatgg ctgatagttt tatcttgccg
     5521 aagcgtgctt ttttgttatt gtctttatga aatcttctta taaaacaaat atatagatat
     5581 tttatattga tgagttattt ttatttatac catcaataaa atccgttaac tagcctgttt
     5641 ataagtgaat aaaggaatag ccgatgatcg aacactatat tcgtgggttt gaagaagacc
     5701 ttcgcgagat ccgccatcag atccacgaaa acccggagct gggattacaa gaatttaaaa
     5761 ccagtgcttt ggttgcggaa aaactgcgcc agtggggcta tgaggttgag caagggctgg
     5821 cgacaaccgg cgtggtagcc acgttaaaag tgggcgacgg agagaaaagt attggcctgc
     5881 gggcggatat ggatgcgttg ccgatttatg aaaatagtgg caagccctgg gccagtaaac
     5941 atcctggttt aatgcacgcc tgcgggcatg acgggcatac caccatactg ctgggggctg
     6001 cgcgttattt tgccgaaacc cgccgtttta gcggcacgtt acgccttatc ttccagcctg
     6061 cggaagagat gattaacggc ggtgagatca tggttaaaga ggggcttttt gatcgtttcc
     6121 cctgcgatgt cattttcggg atgcacaaca tgccgggttt gccggtcggc aagtttttct
     6181 tccagcctgg ggcgctgatg gcgtcaatgg atcagttcca tattacggtt cgtggttgtg
     6241 gcggacacgg tgcgatcccg cacaaagcca tcgatccagt gctggttgcc gcacatatca
     6301 ccaccgcatt acaaagcatt gtgtcgcgca atgtcgatcc gctggaagcg gcggtaatta
     6361 ccgtcggcag cattgttgcg ggtgaggcgg ctaacgtcat tcctgacagt gctgagatga
     6421 aaatcagtgt ccgctctctt agccgcgata cccgacaact tcttctgacc cgtattcctg
     6481 ctcttgcaca agcccaggct gccagctttg gcgctacggc tgaggttacg catgttaacg
     6541 gtacaccggt attagtaaat gacgaggaga tggcgcgctt cgcctggcag gtggcgtgca
     6601 aaacatttgg tgaagatcgg gctgagtttg gcatcaaacc cctgatgggc agtgaagatt
     6661 tctctttcat gctggaagcc caacccaaag gcggtttcct gttattcggt aatggcgatg
     6721 tcggggaagg tgcctgcatg gtgcataacc ccggttacga ctttaacgat gcaagcctgg
     6781 tcccggcaag cagctactgg ggcgcactgg ttgaagcctg gctgcaataa ataaaacaac
     6841 acacaacaac actttttggc aaggaacatg gaatgacggc gatcgtaaat gaaaccgtga
     6901 gagtgaagaa aaagcccagc ggcaggcggg tgatctttgc ctcggcgttt ggcaatgcgc
     6961 tcgaattttt tgattttggc gtttacaact tttttgtggt ctatatcagt acgcttttct
     7021 tcccacccag tgccgatcat aacgtcgcgc tgttactggc atttgcaacg tttggtgtga
     7081 gcttttttat gcgtccgctc ggtgggatta tcgttggtgc ctgggccgat cgcttcgggc
     7141 gtaaaccggc gatggtgttc actattgctc tgatgagcct cggtacgctg atgatcggca
     7201 tcgcaccgac gtatgaaacg gcgggctatt ggggaactgc aacgctggtg ctggcgcgtc
     7261 taattcaggg cgttgcggca ggtggtgaag tcggggcttc aatgtcgcta ctggtggagt
     7321 cagctccggc aaatcgtcgc ggcttttaca gcagttggtc actggcgacc caagggctgg
     7381 caacgacctt cggtggggtt gttgctttag gtctgagcgc atggctaccg ttcgccacag
     7441 gttcagaaac cgtaatggca gaatggggct ggcgtgtgcc gttttttatt ggcgtgttat
     7501 tagcacctat cggttgctgg ttgcgtctga gcctggaaaa tgacgtacct gagccagtgc
     7561 gcaataaaaa agctgctacc agcgaaagcg cgttttctct gctcctgcaa cataaggcga
     7621 ctatcgttaa tggtgtttta ctcgctattg gtagcacggt cgcaacctat atctccctct
     7681 tttattacgg cacctgggcg gcgaagtatc tggcgatgcc tcaacattat tctcatgcag
     7741 cgatgttact ggcgggcgtt gttacgtttg tgggcgcgct gttggtgggg atgctatgtg
     7801 attcggttgg gcgtaaaaag ctgattttaa tctcccgtgt gatggtgctt ctctgtagct
     7861 ggccgtcatt ctggctgttg gtgaattacc caagccctgg catgttgctg acggtggttt
     7921 tcgtgatggt cagctttacc acgcttggcg gtgtgccagt gatgttgttg atctctgaac
     7981 tgttgccgaa acggattcgg gcattaggtt tcgcgctggt ttatagcatt ggtgtggcaa
     8041 tctttggcgg ttttgctcaa tatttcgcta cgcaatccat cgtgttgctg gatagtctga
     8101 cggctccggc gtggtatctg gggggaggca cgttgctgtc gatgctggcg ttgttgtacg
     8161 taaaagaacc ggccaaagaa ttgcagtaaa tatgttttat tgcgttaccg ttcattcaca
     8221 acactggagc aatccagtat gttcattctc tggtatagtg ccagcagtac ttttggcaag
     8281 gattcagaca tcgtgatggg cgtaagagcg caacaaaaag aaaaaacccg ccgttcgctg
     8341 gtggaagccg catttagcca attaagtgct gagcgcagct tcgccagcct gagtttgcgt
     8401 gaagtggcac gagaagcagg cattgctccc acctcttttt atcggcattt ccgcgacgta
     8461 gacgaactgg gtctgaccat ggttgatgag agcggtttaa tgctacgcca actcatgcgt
     8521 caggcgcgtc agcgtatcgc caaaggcggg agtgtgatcc gcacctcggt ctccacattt
     8581 atggagttca tcggtaataa tcctaacgcc ttccggttat tattgcggga acgctccggc
     8641 acctccgctg cgtttcgtgc cgccgttgcg cgtgaaattc agcacttcat tgcggaactt
     8701 gcggactatc tggaactcga aaaccatatg ccgcgtgcgt ttactgaagc gcaagccgaa
     8761 gcaatggtga cgattgtctt cagtgcgggg gccgaggcgt tggatgtcgg cgtcgagcaa
     8821 cgccggcaat tagaagagcg actggtactg cagctgcgaa tgatttcgaa aggggcttat
     8881 tactggtatc gccgtgaaca agagaaaacc gcaattattc cgggaaatgt gaaggacgag
     8941 taatgaaaca agcaaatcaa gatagaggta cgctgctgct agcgttggtt gctggcttat
     9001 cgatcaatgg tacttttgca gcgctgttta gctccattgt accattttct gtattcccga
     9061 ttatttccct ggtgttgaca gtttactgcc tgcatcaacg ttatcttaat cgcactatgc
     9121 cggtaggctt gccgggtctg gcagctgctt gttttattct cggcgtactg ctgtacagca
     9181 cggtagttcg tgcggaatat ccggatatcg gctctaactt cttcccggca gtactgtccg
     9241 tcattatggt gttctggatt ggcgcgaaga tgcgtaaccg taagcaggaa gttgctgagt
     9301 aatcggtatc atgccggatg caagcgcatc cggcatgggt tttacttcgc ggtcagtaat
     9361 acgccgcact ccatatggtg cgtgtagggg aactgatcaa atagagccag acgttcgacc
     9421 ttgtgcgtct ggcttaatgt ttccagattc ttgcataacg tttccggatt gcaggagata
     9481 tacagaatac gtggatacgc ctgcaccatt ttctcggttt cactgtccag accgctgcgc
     9541 ggtgggtcga caaaaatggt ttcgcactga taactcttta agtcgattcc ttgcaggcgg
     9601 ttaaactcgc gcacaccatt catcgcctga gtaaattctt ctgccgccat acgaataatt
     9661 tgtacgttat caatatggtt agcggcaatg ttgtattgcg cggcggcgac tgacggcttg
     9721 gcgatttcgg tggctaatac ccgatcaaaa ttgcgtgcca gcgctaatga aaagttaccg
     9781 ttgccgcagt atagctccag taaatcgcct tttgagcctt tggttacgtc cagcgcccat
     9841 tccagcatct gaatattcat cgccgcgttc ggttgggtaa agctgttttc tacctgacgg
     9901 tagatcatct ctttccccgc gatcggcaga cgttcgtcga tgtaatcctg atccagcgcg
     9961 attttggttt tcgttgcccg accaatcaga tgcacattca gattctgcgc gcgcagtgca
    10021 tcgcgcaggg cttccgcctg ctgacgccac tcatcatcca gctttttatg gtacagcaag
    10081 gaaacaaccg cttgattgct tagcgtagtg aggtaatcaa tctggaacaa cttgtggcgc
    10141 agaatgggat tattacgcac acccgcaatc atcgccgtca tcaactggtt gataagttca
    10201 ctggcggcgg ggaagctatc cacacggata cgacttttgg tttgttgatc gaaaatgatg
    10261 tggtacaggt catcgccatc gtgccagatg cggaactccg cacgcatccg ataatgactg
    10321 accggcgagc gaaacacttc cggaaccagg tcagaaaacg gtgccatcat actttgcaaa
    10381 cgtaccactt tttcggctaa ctgcgcttca tactgttctg ttggaaggtg ttcgggggtc
    10441 atgatgtatc ctgaaaaatt aaagtacgcg gcgattgtag ggattgctca tcagatgtcc
    10501 agatcttgat gaattcctat ttgtgagcta cgtctggaca gtaacttgtt acaacctgta
    10561 gcatccactt gccggtcctg tgagttaata gggaatccag tgcgaatctg gagctgacgc
    10621 gcagcggtaa ggaaaggtgc gatgattgcg ttatgcggac actgccatcc ggtgggaagt
    10681 catcatctct tagtatctta gatacccctc caagcccgaa gacctgccgg ccaacgtcgc
    10741 atctggttct catcatcgcg taatattgat gaaacctgcg gcatccttct tctattgtgg
    10801 atgctttaca atgattaaaa aagcttcgct gctgacggcg tgttccgtca cggcattttc
    10861 cgcttgggca caggatacca gcccggatac tctcgtcgtt actgctaacc gttttgaaca
    10921 accgcgcagc actgtgcttg cgccaactac tgttgtgacg cgtcaggata tcgaccgctg
    10981 gcagtcgacc tcggtcaatg atgtgctgcg ccgtcttccg ggcgtggata tcacccaaaa
    11041 cggcggttca ggtcagctct catctatttt tattcgcggt acaaatgcca gtcatgtgtt
    11101 ggtgttaatt gatggcgtcc gcctgaattt ggcgggggtg agtggttctg ctgatcttag
    11161 tcagttccct attgcgcttg tccagcgtgt tgaatatatc cgtgggccgc gctccgctgt
    11221 ttatggttca gatgcaatag gcggggtcgt gaatatcatc acgacgcgcg atcaccccgg
    11281 aacggaaatt tcagcagggt ggggaagcaa tagttatcag aactatgatg tctctacaca
    11341 gcaacaactg ggggataaga cacgggtaac gctgttgggc gattatgccc atactcatgg
    11401 ttatgatgtt gtcgcctatg gtaataccgg aacgcaagcg cagccagata acgatggttt
    11461 tttaagtaaa acgctttatg gcgcgctgga gcataatttt actgatgtct ggagcggctt
    11521 tgtgcgcggc tatggctatg ataatcgtac caattatgac gcatattatt ctcccggctt
    11581 gccactggtc gatacccgta aactctatag ccaaagttgg gatgccggac tgcgctataa
    11641 cggcgaactg attaaatcac aactgattac cagctacagc catagcaaag attacaacta
    11701 cgatccgcat tatggtcgtt atgattcgtc cgcgacgctc gatgagatga aacagtacac
    11761 cgtccagtgg gcaaacaaca tcatcattgg ccacggtaat attggtgcgg gtgttgactg
    11821 gcagaagcag agcacggcac cgggcacagc ttatgttgag gatggatatg atcaacgtaa
    11881 taccggcatc tatctgactg ggctgcagca agtcggcgat tttacttttg aaggcgcagg
    11941 acgcagcgac gataattctc agtttggtcg tcatggtacc tggcaaacca gcgccggttg
    12001 ggagttcatc gaaggttatc gctttattgc ttcctacgga acatcttata aggcaccaaa
    12061 tcttgggcaa ctgtacggct cctacggtaa tccgaatctg aatcctgaga aaagcaaaca
    12121 atgggaaggc gcgtttgaag gcttaactgc tggggttaac tggcgtattt ccggatatcg
    12181 taacgatgtc agtgacttga tcgattatga tgatcacacc ctgaaatatt acaatgaagg
    12241 gaaagcgcgg attaagggtg tcgaggcgac cgctaatttt gataccggac cactgacgca
    12301 tactgtgagt tatgattatg ttgatgcgcg caatgcgatt actgacacgc cgttgttacg
    12361 ccgtgctaaa cagcaggtga aataccagct cgactggcag ttgtatgact tcgactgggg
    12421 gattacttat cagtatttag gcactcgcta tgataaggat tactcctctt atccttatca
    12481 aaccgttaaa atgggcggtg tgagcttgtg ggatcttgcg gttgcgtatc cggtcacctc
    12541 tcacctgaca gttcgtggta aaatagccaa cctgttcgac aaagattatg agacagtcta
    12601 tggctaccaa actgcaggac gggaatacac cttgtctggc agctacacct tctgaaccac
    12661 gtcccaccgt gctggtgttt gactccggcg tcggtgggtt gtcggtctat gacgagatcc
    12721 ggcatctctt accggatctc cattacattt atgctttcga taacgtcgct ttcccgtatg
    12781 gcgaaaaaag cgaagtgttt attgttgagc gagtggtgga aattgtcacc gcagtgcaag
    12841 aacgttatcc ccttgcgctg gctgtggtcg cttgcaacac cgccagtacc gtttcacttc
    12901 ctgcattacg cgaaaagttc gacttcccgg ttgttggtgt cgtgccggcg attaaacctg
    12961 ctgcacgtct gacggcaaat ggcattgtcg gattactggc aacccgcgga actgttaaac
    13021 gttcttatac tcatgagctg atcgcgcgtt tcgctaatga atgccagata gaaatgctgg
    13081 gctcggcaga gatggttgag ttggctgaag cgaagctgca tggcgaagat gtttctctgg
    13141 atgcgctaaa acgtatctta cgcccgtggt taagaatgaa agagccgcca gatacagttg
    13201 tattgggttg cacccatttc cctctactac aagaagaact attacaagtg ctgccagagg
    13261 gaacccggct ggtggattct ggcgcagcga ttgctcgccg aacggcctgg ttgttagagc
    13321 atgaagcccc ggatgcaaaa tctgccgatg cgaatattgc attttgtatg gcaatgacgc
    13381 cagaagctga acaattattg cccgttttac agcgttacgg cttcgaaacg ctcgaaaaac
    13441 tggcagtttt agattgattt ggttgaatg
//
LOCUS       DAZGJT010000113        13233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZGJT010000113 DAZGJT010000000
VERSION     DAZGJT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.113"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            153..1652
                     /gene="pitB"
                     /locus_tag="ACHKFK_003920"
                     /pseudo
     CDS             153..1652
                     /gene="pitB"
                     /locus_tag="ACHKFK_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
     gene            complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHKFK_003921"
     CDS             complement(1701..2393)
                     /gene="yghT"
                     /locus_tag="ACHKFK_003921"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9054159.1"
                     /translation="MQSIIPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEKVLVDAEK
                     AITDFMTARGYH"
     gene            2568..3281
                     /gene="yghS"
                     /locus_tag="ACHKFK_003922"
     CDS             2568..3281
                     /gene="yghS"
                     /locus_tag="ACHKFK_003922"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO9054160.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELASRMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKNQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            3313..4071
                     /locus_tag="ACHKFK_003923"
     CDS             3313..4071
                     /locus_tag="ACHKFK_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054161.1"
                     /translation="MDALQTQTVKSTTAPQPNYIPGLIAVVGCDGTGKSTLTNDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISRF
                     LAQRERRLYQKMAQYRPELIIRLGIDIDTAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4117..5466
                     /locus_tag="ACHKFK_003924"
     CDS             4117..5466
                     /locus_tag="ACHKFK_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO9054162.1"
                     /translation="MAVFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNDPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNSTGEKS"
     gene            5466..6302
                     /locus_tag="ACHKFK_003925"
     CDS             5466..6302
                     /locus_tag="ACHKFK_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000984967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054163.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLKFWLDGVNRQELLRLIRKQAKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGHFQDGFRNGKKNIVSFYGNLLRVYLSSPVWWLVNYSLRHSQ
                     LETVNGFIAYRQKQMYILKEIIAKPQLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALLISRFLAAINGLMGDKHDDMVADDMDNRRSYNAPVALDSDIRQRLKLLISRFPLYP
                     EQ"
     gene            6302..6862
                     /locus_tag="ACHKFK_003926"
     CDS             6302..6862
                     /locus_tag="ACHKFK_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO9054164.1"
                     /translation="MPSRWIVALIIALLAPGAAAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLTGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFSQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGLGRVAWQLPEQSSTILVLNKAGRI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6893..7963
                     /gene="lptF"
                     /locus_tag="ACHKFK_003927"
     CDS             6893..7963
                     /gene="lptF"
                     /locus_tag="ACHKFK_003927"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO9054165.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYASVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFVAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFPR"
     gene            7960..9039
                     /gene="lptG"
                     /locus_tag="ACHKFK_003928"
     CDS             7960..9039
                     /gene="lptG"
                     /locus_tag="ACHKFK_003928"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO9054166.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDNDIIGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSTFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWHDRQH"
     gene            complement(9074..10246)
                     /locus_tag="ACHKFK_003929"
     CDS             complement(9074..10246)
                     /locus_tag="ACHKFK_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO9054167.1"
                     /translation="MGLYDKYARLAGERLQFSDYGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAIREGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISTLADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDVN
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLVEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIASQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10246..10494)
                     /locus_tag="ACHKFK_003930"
     CDS             complement(10246..10494)
                     /locus_tag="ACHKFK_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO9054168.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10526..11440)
                     /locus_tag="ACHKFK_003931"
     CDS             complement(10526..11440)
                     /locus_tag="ACHKFK_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO9054169.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGTPETQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVQQLVADVRCGSVRMVGIPQPLLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11437..13161)
                     /locus_tag="ACHKFK_003932"
     CDS             complement(11437..13161)
                     /locus_tag="ACHKFK_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO9054170.1"
                     /translation="MSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLE
                     DQLEYQTLKARAEAGAKRLLSLNLKKGDRVALIAETSSGFVEAFFACQYAGLVAVPLA
                     IPMGVGQRDSWSAKLRGLLASCQPAAIITGDEWLPLVNAATHNNNPELHVLSHAWFKA
                     LPEADVVLQRPVPNDIAYLQYTSGSTRFPRGVIITHHEVMANLRAISHDGIKLRPGDR
                     CVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPP
                     FGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPC
                     YGLAENALAVSFSDEACGVVVNEVDRDILEYQGKAVAPSAETRAVSTFVNCGKALPEH
                     GIEIRNEADIPVAERVIGHICISGPSLMSGYFGDQVSQDEIATTGWLDTGDLGYLLDG
                     YLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRIS
                     DEARRGQLIHALAARIQSEFGVTADIVLLPPHSIPRTSSGKPARAEAKKRYQKAYAAS
                     LHVQESLA"
BASE COUNT         3192 a         3246 c         3421 g         3374 t
ORIGIN      
        1 tatttttaaa ggttttgaat gaaggcttat tgtctgatac acaagaaata acacgctttt
       61 tatcgttaaa aaatgatatt tcactttgcc cacgccgtga aagtcactga taatgcctcc
      121 gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt ggccttgata
      181 tatatacggg gcttttgtta ttacttgctc tggcatttgt gttgttctat gaagcaatca
      241 atggttttca tgacacggcg aatgcggtgg caaccgttat ttatacccgt gccatgcaac
      301 cacagcttgc tgtggtcatg gcggcatttt ttaacttttt tggcgtattg cttggcgggc
      361 ttagcgttgc ctatgccatc gtccatatgt tgccaaccga tttgttgctg aatatggggt
      421 caactcacgg tctggcgatg gtcttttcca tgctgctggc ggcgattatc tggaatctgg
      481 gaacgtggtt ctttggttta ccggcctcca gttcgcacac tttgattggt gcgattatcg
      541 gcattggttt aaccaacgcg ctgttaaatg gttcgtcggt gatggatgcg ttaaacctgc
      601 gcgaagtcac caagattttc tcttcactca ttgtctctcc catcgtgggg ctggtgattg
      661 cgggggggct aatttttctg ctacgccgtt tctggagtgg gacgaaaaag cgtgaccgta
      721 ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaagccg ccattctgga
      781 cgcgtattgc cctcattgtt tccgctgcgg gcgtggcgtt ttcgcacggc gcgaacgacg
      841 ggcagaaagg aattggtctg gtgatgctgg tgctggtggg gattgctccc gccgggtttg
      901 tcgtcaatat gaatgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc gtcaccaact
      961 tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattacg atggaacctc
     1021 cattgcctgc gacatcgact gatggcacgc aagtaacaga gtttcactgt catccggcaa
     1081 atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg gaaagttacg
     1141 agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc atctctgata
     1201 cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac ctgctgaaaa
     1261 aactgcgcag cgatatgtta agcaccattg agtacgcacc ggtatggatc atcatggcgg
     1321 tagcactggc gctcggcatc ggcaccatga ttggctggcg tcgtgtagcg atgaccatcg
     1381 gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca caaatgacgg
     1441 cggcagtgtc tatcggcctt gccagttata ttgggatgcc cgtctccaca acacacgtac
     1501 tctcatctgc cgtagcagga acgatggtgg tggacggcgg tgggttacag cgtaaaacgg
     1561 taaccagcat cctgatggcg tgagtattta ctttaccggc ggcgattttt ctttctggtg
     1621 ggttgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg gtcttggcga
     1681 tcgggcattt ccatttttca ctagtgataa ccacgcgcgg tcataaaatc cgtaatcgct
     1741 ttttctgcat caaccagcac cttttccagt ggctgattgg cgtcgatatc aaccagttgt
     1801 gcaccaccaa aggttaactg tggcgttatg gcaatcttcc tcgccagcga ttcccgttta
     1861 tggtcgggtt tacgcgcgca ggcaacttca aggtcaacat tgagtttgat gaccagatcg
     1921 ggcttgtggc tcgccatcca gtgaaatgct ttacgttcct gacttgccag ccatgagaca
     1981 aaacgaccac cttcaacgtt aggcggaaac accgtaccat cataagcgcc aggaatctgg
     2041 tcctgcggat aacggtcggt taaaacaatt aacccgcgac gacgacaggc aagcatatga
     2101 cgaaagcgca gtaagcgacg ggcgacaaac gctgtaatta ccagcgccgg aactggtcca
     2161 ggcaattttt ttgctgtttt tacctgattt cgttcaattg ttttatgtaa ggattttccc
     2221 atcaacggta attttgtcac tgcacgaccg acatttccgg cctgttttcc taaatgaacc
     2281 ctttcggcag caccatattt ttcgacaacg gtaataagat gttcacacac cgttgacttg
     2341 cctgaaccat cgctaccaat aacggcaatt aatggaggta ttattgactg catgaatata
     2401 tccttaaatt taaatatcca tccaaaatat ttatttggtt aatatgtttt tgatgaaagc
     2461 gtaattcagg tcaatgtcac aattaaccat tgtcacaata aggttgaacg gatattctga
     2521 gcacggacca cgtaatattc aatacattat ttgctgccgg gtttttcgtg aatcaacgta
     2581 atatgtcaat aattaattcc acgccagtgc gtgttattgc cattgtagga tgtgatggtt
     2641 caggtaaatc gaccctcacg gcaagcctgg taaatgaact ggcatcaaga atgccaacag
     2701 aacacattta tctcgggcaa tcgtccgggc gaattggcga atggatttca cagctacctg
     2761 ttattggcgc accttttggg cgttatttgc gaagtaaagc ggcacatgtg cacgaaaagc
     2821 cctcaacacc gcctggcaat attactgcgc tggttatcta tctgctttcc tgctggcggg
     2881 cgtacaagtt tcgcaaaatg ttgtgtaaaa accagcaagg ctatctgctc atcaccgacc
     2941 gctacccgca ggttgaagtg ccgggtttcc gctttgatgg cccgcaattg gcaaaaacca
     3001 cgggcggtaa cggttggata aaaatgttaa ggcagcgtga gctgaagctg taccaatgga
     3061 tggcgtctta tttgcctgta ttgttgattc gtcttggcat tgatgaacaa accgcgtttg
     3121 cgcgtaaacc tgaccaccaa ctggcagcgt tacaggaaaa aatcgccgtt acgccgcaac
     3181 tgacattcaa tggcgccagg atccttgaac tggatggtcg gcaaccagcc gatgaaattc
     3241 tgcaagcgtc actacgcgca attcacgccg cgctttcctg atcaccaaaa tggatagggg
     3301 atgattaaac gaatggatgc actacaaaca caaactgtta agagtacaac cgcgccgcag
     3361 cccaactaca ttccggggct gattgcggtg gtcgggtgtg atggcaccgg taaatccaca
     3421 ctgacaaatg acctggtgaa atcgctgcaa caacattggc aaaccgagcg gcgctatctg
     3481 gggctgctct ccggcgaaga cggcgacaaa atcaaacgat tgccgttggt tggtgtctgg
     3541 ctcgaacggc gactggcggc caaatcctcg aaaacccaaa gcatgaaaac caaatctccg
     3601 gcgctatggg cggcggtgat tatgtactgc ttctcgctgc gaagaatggc gaatctgcgc
     3661 aaggttcagc gactggcgca aagtggcgtt ctggtggtca gcgatcgctt cccgcaggct
     3721 gaaatttcgg gcttttatta tgacggaccg gggattggcg tcgaacgtgc gaccgggaaa
     3781 atcagcaggt ttctggcgca gcgtgaacgg cgtttatacc aaaaaatggc gcaataccgt
     3841 cctgaattaa tcattcgctt ggggattgat attgatactg ccatctcccg caagcctgac
     3901 catgactatg ccgagctgca ggacaaaatc ggtgttatgt cgacgattgg ctataacggc
     3961 acaaaaattc ttgagattga ttccagagcg ccttacagcg aggtgctgga acaggcacag
     4021 aaggcggttt ctctggttgc catcgtttct gaccgccgaa gtttaactta aggctggcgg
     4081 gatacttttt actatcaacg ataaggtcag gctgaattgg cggtttttaa catcaaacat
     4141 tggtttgcag atggcgcgtt tcgcaccatt attcgcaata gcgcctggtt aggctccagt
     4201 aatgttgtga gcgctttgct ggggctgttg gcgctctcgt gtgccggtaa agggatgacg
     4261 cccgccatgt ttggcgtact ggtaattgtg caatcgtacg ccaagtcgat cagcgatttt
     4321 attaagtttc agacatggca gctggtggtt cagtacggaa cgccagcttt gaccaacaat
     4381 gatccgcagc aatttcgcaa tgttgtctca ttttccttct cgctggatat cgtcagcggc
     4441 gcggtggcga ttgtcggcgg cattgcctta ctgccattcc tttcccattc attaggtctg
     4501 gatgaccaaa gcttttggct ggcggcgctc tattgcacgc tcattccttc aatggcttcc
     4561 tccacgccga ccggcattct gcgtgcggta gatcgctttg atttgattgc tgtacagcag
     4621 gcgacgaaac cttttctgcg cgcagcgggg agcgtcgtag cctggtattt tgactttggt
     4681 tttgcgggtt ttgttattgc ctggtacgtg tcgaatctgg ttggcggcac catgtactgg
     4741 tggtttgccg cgcgcgaatt acgccgcaga aatatccata acgccttcaa attgaatctg
     4801 tttgagtctg cccgacacat taaaggcgcg tggagttttg tctggtcaac caacattgcc
     4861 cactccatct ggtcggcgcg taactcgtgc agcacagtgt tggtggggat cgtgttagga
     4921 cccgctgctg ccgggttatt taaaatcgcc atgacattct tcgatgccgc cggaacgcca
     4981 gcgggtttac tgggcaaaag tttttatcca gaggtgatgc gtttagatcc acgcaccacc
     5041 agaccgtggt tgttaggcgt gaagtccggt ttactggcgg gcggaatcgg tattctggtg
     5101 gcgctcgcgg tgttgatcgt cggcaagccg ctcatttcgc tggtgtttgg cgttaagtat
     5161 ctcgaagcgt atgacctgat tcaggtgatg ctgggcgcaa ttgtgatttc gatgctgggc
     5221 ttcccacagg agtcattgct attgatggcg ggaaaacagc gcgcttttct cgtggcgcaa
     5281 accatcgcct caatcggcta catcgtattg ctgttcatgt tctgtcatct gtttggcgtg
     5341 ctgggggctg catttgctta cttcggtggg caatgtctgg atgtggtgct ctcgctaatt
     5401 ccgaccttaa aggcattttt tcagcgccat tccttgcttt ataactcaac cggagagaaa
     5461 tcctgatgat gagaaaatat ttcccgcttg aggcgagcga gcgtctattt gttgcgattg
     5521 aagaagatga tgttgttgat gcacaggtca gtttgccgcc gactatcgca ctaagttgta
     5581 ccaccgaaat catccatgat aattacgcgt tatgcctgaa attctggctg gatggtgtta
     5641 atcgtcagga gttactgcgt ttaatccgca aacaggcgaa aggcgatgag ctgacgacag
     5701 acgagcgcaa acagtttaaa tacatgcgag cgcgctacaa gcatctgcgt tttgcgcaac
     5761 ggctgtattt aaagaagcat caggccggat ttttattcgg caaaacgacc gtttttcttg
     5821 ggcattttca ggatggcttt cgtaacggta agaaaaatat tgtgtcgttt tacggtaacc
     5881 tgttacgtgt ttatttgagc tcccctgtct ggtggctggt gaactattca ttgcgccata
     5941 gccagttaga aaccgtgaac ggttttatcg cctaccgcca gaagcaaatg tacatcctga
     6001 aagagattat tgctaaaccg caactaactg gcagggaatt tcatgatgtg cgaaaaatca
     6061 tcagccagca ggtttcttat tacgacacct taagatcgct tgatcctgaa aataaagagg
     6121 cgttactgat ttcccgtttt ctcgccgcca ttaatggtct gatgggggat aagcacgacg
     6181 acatggtggc ggatgatatg gataaccgtc ggtcatataa tgcaccggtg gcgctggata
     6241 gcgatattcg ccagcgttta aaactgctta tttcgcgttt cccactatat ccggagcaat
     6301 aatgccatct cgttggattg tcgctctgat catcgcactc ttagcgccag gcgcggcggc
     6361 acacaatttc gtcactggca aaacggttac tcctgtttat atccaggaag gcggcgagct
     6421 gcttctgaat agcgacgacg aaattcacta ccaaaagtgg aacagcacac aattgacggg
     6481 aaaagtccgc attattcagt atatcgccgg acgtaagtct gcgaagaaaa agaactcact
     6541 gttaattaaa gcggttgaag cggcaaactt ttcgcaggat cgttttcagc ccaccactat
     6601 tgtgaacacc gatgatgcca tctttggtac tggctatttt gtcgttggca aaatcgaaaa
     6661 aaataaacgc cgctatccgt gggcgcaatt cgttattgac ggcaatggtc tgggacgcgt
     6721 tgcctggcaa ttgccggagc agagttcaac cattctggta ttgaataaag ccgggcgaat
     6781 tcagtgggca aaggacggca gcctgacacc ggaagaggta gatcatgtca ttgcgctggc
     6841 gcagaaactg atcaatgaat aaaaaattca aaggattgag ggcgttagca agatgaagct
     6901 ggttgaacac tacatcatgc gtggtacgcg ccgtctggtg ctgattatcg tcggttttct
     6961 gatctttatt ttcgccagct actccgcaca gcgttatctg accgaagcgg caaatgggac
     7021 attagctctt gatgttgtgc tggatatcgt tttctacaaa gtgctgattg cactggagat
     7081 gctgttacct gttggactat atgcttcagt tggcgtgacg ctagggcaaa tgtataccga
     7141 ctcggaaatt accgctatct ccgcggcggg gggcagtccg ggacgtttgt acaaagccgt
     7201 tctttatctg gcgataccgt taagtatttt tgtcaccctt ctgtcgatgt atggtcgtcc
     7261 ctgggcttat gcgcagattt atcaactgga gcaacaatcg cagtcggagc tggatgttcg
     7321 tcagttgcgg gcaaagaaat ttaacactaa cgataacgga cgaatgatcc tttcgcagac
     7381 ggttgatcag gataataatc gcctgactga cgcgctgatt tatacttcta ctgccaatcg
     7441 aacccgcatt ttccgcgccc gttcggttga tgtggttgac ccatcacccg agaaaccgac
     7501 cgttatgttg cataacggta ccgcctatct tctcgatcat caggggcgtg acgacaacga
     7561 acagatctac cgtaatctgc aattgcatct caatccgctg gatcaaagcc ctaacgtcaa
     7621 acgcaaagca aaatcggtca cggagctggc acgctccgtc tttccagccg atcatgccga
     7681 actgcaatgg cgacaaagcc gtggcctgac agcattgttg atggcgctgc tggccatttc
     7741 attaagtcgg gtaaaaccgc ggcaggggcg attttcaacg ctactgccac tgacgttgct
     7801 gtttgttgcc attttttatg gcggcgacgt ctgtcgtacg ctggtggcta acggcgcgat
     7861 tcccctcatt cctggtttgt ggttagtgcc cggactcatg ctaatgggcc tgttgatgct
     7921 ggtcgcacgc gacttctcct tgctgcagaa atttccccga tgaatgtttt cagtcgctat
     7981 ttaatccgtc acctctttct cggttttgcc gccgccgcag ggctattgct gccgcttttt
     8041 accaccttca acctgattaa cgaactggat gatgtcagtc cgggcggtta tcgctggact
     8101 caggcagtac tggtggtgct gatgacctta ccgcgcacac tggtcgaact ttcgccgttt
     8161 atcgcgttat tgggggggat tgttggtctc gggcagttat cgaaaaacag tgagcttacc
     8221 gccattcgca gcacggggtt ttctatcttc cgcattgcac tggtggcgct ggttgcaggg
     8281 atactgtgga ctgtttcgtt aggcgcgatt gatgagtggg tggcgtcacc attgcagcaa
     8341 caggcgctgc aaatcaaatc gactgccacc gcgttggggg aggacaatga cattatcggc
     8401 aatatgcttt gggccaggcg cggtaatgaa tttgtgacgg tgaaatcgct gaacgagcag
     8461 ggccagccgg tgggcgtgga gatttttcat taccgcgacg atctctcgct cgaatcctac
     8521 atttatgcgc gcagtgccac cattgaagac gataaaacgt ggatcctgca tggtgtgaat
     8581 cataaaaaat ggctgaatgg caaagaaacg ctggaaacat ctgataatct tgcctggcaa
     8641 tcgaccttca ccagtatgaa tcttgaagag ttatcgatgc cggggaatac tttttctgtc
     8701 cgtcagctta atcattacat ccattatttg caggaaaccg ggcaacccag cagcgaatac
     8761 cgccttgcac tgtgggaaaa actggggcaa cccatcttga ctctggcgat gattttgctg
     8821 gctgtgccgt ttacctttag tgccccgcgc tcgccaggga tgggtagccg tctcgctgta
     8881 ggcgtcatcg ttggtttact cacctggatc agctatcaaa tcatggtcaa tctgggattg
     8941 ctatttgcgt tgagcgcacc tgttaccgcg ctcggtttac cggtagcgtt tgtgctggtg
     9001 gcgttgagcc tggtgtattg gcatgacaga caacattaaa cccgccgtgg cgcaacacca
     9061 cggcgggttc acattactgc ttcagagtag caaatgcctg aatgatggca tcgatttgct
     9121 cacgactgtg ggcagcgttt acgctacagc gcagcagcgt gatgccagca ggcgcggcag
     9181 gtggcaggat aagattcaca taaacgccca gcgaaataag ctcgcgccag atgcgtaaac
     9241 cttcctcttt cgaaccgatt attactggca ctaccgggct aatatgtgaa cttaactcgt
     9301 aacccagttt ttgcagaccg tcatacagat ggttggcgtt atccatcagc ttttgccgca
     9361 gctctggttg tgaggcaatg gttcttaacg acgagcgcac ggtcgcaatg caggacggag
     9421 aaggagaggc ggtaaaaata tacgggcggc tgccgtagcg cagcacttcc atcgcttccg
     9481 aaccgacagc aaaaccgcca atcgaggcca gacttttact gaaggtgccg acgataatat
     9541 ccacgtcgtc ttcaacgcca acggcttcaa ccagtccgcg ccccgttgcg cctaacacgc
     9601 caaacgaatg tgcttcatcg acaattaaat atccgccgag acgacgtttg atatcaacaa
     9661 tttctgccag cggcgcaacg tcaccgagca tactgtagat gccttcaacg ataataatcg
     9721 cctctttcgc ccgttcaccg aggcggacca tgcgccgttc cagatcgttg acgtcattat
     9781 ggcggaaacg aataatctcc gcgccgccga gagaacaggc gtcataaata ctggcgtggc
     9841 tgtcggcatc cagtaacacc acggcgttat ggtcggcaag tgtactgatg acgcctaaat
     9901 tagcggtata accggttgaa aaaacgatag cggtagggcg attgaaaaac tcggcaatct
     9961 ctttctctaa cgccagatgc ggcgcataac tgccgtttgc catacgagaa ccggtggtgc
    10021 cggtgccttg ggccgctaac gctgcctgac cttcgcggat agcatcatga ttaaaggtta
    10081 aaccgagata attattggtc ccggccagaa tcactttttt attgccgatc cggccttcgg
    10141 tagcggaata cacttcatca atacaggtgc caaatggtgt taacccgtag tcactaaatt
    10201 gcaggcgctc gccagcaagg cgcgcatatt tatcgtatag ccccattatt tattctccag
    10261 ccatggaagt agagtttcca ttaattgcgc aggggttttg acatccagca gaatattaat
    10321 aggaatggaa atatcgaatc tgtcttccag catcattaag agatccatca ctttgatcga
    10381 ctccaggcca agatcgttga cgagatcgct gtcgggctta atttccacac cgttttcgac
    10441 caaatcctgt aaacaggaaa ggatgtaatc cattactatt tcacgattta ccatagaaac
    10501 attaacacac ctttaaacaa gacaatcaaa atagcccgtt gcgtaatgcg tgttccaggc
    10561 taatcccggg aaaccaatta atatcttcag aaatacggtt attacttgcc gaccagtcgg
    10621 catgggttaa ttcacggatt ttcgaacgag tcagcatcgg ttctttaccc gccagacggc
    10681 tcaacgcggt actgatatcc gcaaggcagg tgagtagcgg ctgaggaata ccgaccatcc
    10741 gcacggaacc acagcggaca tcggcaacaa gctgttggac acgttgccag tcatagcctc
    10801 cggcgacgcc atcgcataat tcataggttt gcgtctgtac agtttcggcg cttaaccact
    10861 gacccactgc ttgcgcaaaa tcggtgacgt gaagaaaaga gagctgtgtc tctggcgtgc
    10921 caagtcgtgg cagcagaccg cgcagcatcc agtcaaacag cggttttaac tctttatcgc
    10981 cgggaccata cacggctgtc gggcgaaaaa cgcccaacgt aatttcgtca gccattgcag
    11041 tcagccgttg ttcggcgacg tgtttggaat ttgcgtacca ggagagttcg ggatggcgcg
    11101 ccgccagcga agagataaac agaaaacgtt ggcaaaagcc gctctctttt gccgcttgca
    11161 tcaggcgcag gctgccgtca acgttgcagc gggtaaaaat ctcttctttg tgcccgcgca
    11221 cttgcccggc gcaatggacc accgcgctgg ctccggcaac cagttcgcta agcgaatgtg
    11281 tatcttccag cgaaccgcgc acccaagtga gattatcatt gacgtgagcg cgggcagtac
    11341 gcgtcaatgc gcgaacgtga aagccgcggg cgagcaggtt atcaataata tatttaccga
    11401 taaacccggt agcgcccgtc accgcgacag tttgattcat gccagggatt cctgcacatg
    11461 aagactggca gcataagcct tctgataacg ttttttcgct tccgcacggg caggcttgcc
    11521 ggaggatgtt cggggaatac tgtggggcgg caacagaacg atatccgcgg taacgccaaa
    11581 ttcgctttgg atccgagctg ccagcgcgtg gataagctgc ccgcgacgtg cttcgtcgct
    11641 gatccgacac tggatctgca aaatgatttt ttcctgggcg gtaacaaaag caatcgcatc
    11701 gccagaatga atttccggct cctgttccgc aatgtattca atatcctgcg gccagatatt
    11761 acggccacga ataataatca gatctttaat gcgtccggtg acatacagat aaccgtccag
    11821 cagataacca aggtctccgg tgtctaacca gcccgtcgtg gcaatctcgt cttgcgaaac
    11881 ctggtcgcca aagtacccgc tcatcaggct ggggccggag atgcaaatat ggcctatcac
    11941 acgttccgcg acaggtatat ctgcttcatt gcggatttca atgccatgtt ccggcaacgc
    12001 tttaccgcag ttgacgaaag tcgatacggc gcgtgtttca gcactcggcg cgacggcttt
    12061 accctggtat tcgaggatgt cgcgatccac ttcgttaacc acaaccccgc aggcttcatc
    12121 agagaagctg acagccagcg cattttctgc cagtccgtag cacggcatga aagttttatc
    12181 gtcaaagtta acctgacgga aacattcagc gaattgatgg agttgttctg cggatatggg
    12241 ttctgcgcca ataccagcga cgcgccagca ggaaagatcc agttcagcga gatctttttc
    12301 attcacgcgg cgctggcaca attcatagcc aaacggcggt gcaacggaaa cggtgccacg
    12361 atttttactg atcaatttaa gccattgcag aggacgcatg gcaaaatcct gagtgcgcaa
    12421 ataatctact gaaagctgcg tggcgacggg ggtcaggaga aagccgacca gtcccatatc
    12481 atggtagaaa ggcagccagg agacgcagcg gtcgccaggg cgtaatttaa tcccatcgtg
    12541 gcttatagca cgtagattag ccatcacttc gtgatgggtg ataatgacgc cacggggaaa
    12601 acgggtgctg ccggaggtgt actggaggta ggcgatatcg tttggaactg gacgctggag
    12661 cacaacatcg gcttccggta atgccttaaa ccaggcgtgg cttaaaacat gtaattcggg
    12721 gttgttgtta tgcgtcgcgg cattgaccag tggcaaccac tcatcaccag tgataatggc
    12781 tgcgggctgg caacttgcca gtaaaccccg cagtttggcg ctccaggaat cccgctgacc
    12841 tacgcccatt ggaatcgcca acgggacggc gactaagccg gcatactggc aggcaaaaaa
    12901 agcctctaca aacccgctac tcgtttcggc aatcagcgcc acgcgatcgc cttttttcag
    12961 gttcagcgat aacaaccttt tcgcaccagc ttcggcacgc gcttttaacg tctgatattc
    13021 cagttgatct tcaagttggc aacgtctgtc ataaaaattc attccggcgc tactcagagc
    13081 ggcgtagtcc aaagcatcaa ccagcgttgg aaaatcggca tagcgcatag gtagggaatg
    13141 cgtaaagatt ttattagaca tatacaccac gaaaaaattg gatatcaaaa ataaaatgac
    13201 aataatgcgg tggattatat aggcataaaa gat
//
LOCUS       DAZGJT010000114        13037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZGJT010000114 DAZGJT010000000
VERSION     DAZGJT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.114"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..1277)
                     /locus_tag="ACHKFK_003933"
     CDS             complement(72..1277)
                     /locus_tag="ACHKFK_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001161829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HFO9054171.1"
                     /translation="MQGNALTVLLSGKKYLLLQGPMGPFFNDVAEWLESLGRNAVNVV
                     FNGGDRFYCRHRQYLAYYQTPKEFPGWLRDLHRQYDFDTILCFGDCRPLHKEAKRWAK
                     SKGIRFLAFEEGYLRPQFITVEEGGVNAYSSLPRDPDFYRKLPDMPTPHVENLTPSTM
                     KRIGHAMWYYLMGWHYRHEFPRYRHHKSFSPWYEARCWVRAYWRKQLYKVTQRKVLPR
                     LMNELDQRYYLAVLQVYNDSQIRNHSNYNDMRDYINEVMYSFSRKAPKESYLVIKHHP
                     MDRGHRLYRPLIKRLSKEYGLGERVIYVHDLPMPELLRHAKAVVTINSTAGISALIHN
                     KPLKVMGNALYDIKGLTYQGHLHQFWQADFKPDMKLFKKFRGYLLVKTQVNWVYYGGN
                     TTNCQHNIY"
     gene            complement(1312..3339)
                     /locus_tag="ACHKFK_003934"
     CDS             complement(1312..3339)
                     /locus_tag="ACHKFK_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFO9054172.1"
                     /translation="MIGIYSPGIWRIPHLEKFLAQPCQKLSLLRPVPQEVDAIAVWGH
                     RPSAAKPVAIAKAAGKPVIRLEDGFVRSLDLGVNGEPPLSLVVDDCGIYYDASKPSAL
                     EKLVQDKAGNAALADQAREAMHTIVTGDLSKYNLAPAFVADESERSDIVLVVDQTFND
                     MSVTYGNAGPHEFAAMLEAAMAENPQAEIWVKVHPDVLEGKKTGYFADLRATQRVRLI
                     AENVSPQSLLRHVSRVYVVTSQYGFEALLAGKPVTCFGQPWYAGWGLTDDRHPQSALL
                     SARRGSATLEELFAAAYLRYCRYIDPQTGEVSNLFTVLQWLQLQRRHLQQRNGYLWVP
                     GLTLWKSAILKPFLQTATNRLSFSRRCTAASACVVWGVKGEQQWRAEAQRKSLPLWRM
                     EDGFLRSSGLGSDLLPPLSLVLDKRGIYYDATRPSDLEVLLNHNQLTLAQKMRAEKLR
                     QRLVESKLSKYNLGADFSLPAEAKDKKVILVPGQVEDDASIKTGTVSIKSNLELLRTV
                     RERNPHAYIIYKPHPDVLVGNRKGDIPAEQIAELADYQALDADIIQCIQRADEVHTMT
                     SLSGFEALLHGKHVHCYGLPFYAGWGLTVDEHRCPRRERKLTLADLLYQTLIVYPTYI
                     HPTRLQPITVEEAAEYLIKTPRKPMFITRKKAGRVIRYYRKLIMFCKVRFG"
     gene            complement(3336..4076)
                     /gene="kdsB"
                     /locus_tag="ACHKFK_003935"
     CDS             complement(3336..4076)
                     /gene="kdsB"
                     /locus_tag="ACHKFK_003935"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001578967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO9054173.1"
                     /translation="MSKAVIVIPARYGSSRLPGKPLLDIVGKPMIQHVYERALQVAGV
                     AEVWVATDDQRVEKAVQAFGGKAIMTRNDHESGTDRLVEVMHKVEADIYINLQGDEPM
                     IRPRDVETLLQGMRDDPALPVATLCHAISAEEATEPSTVKVVVNTHQDALYFSRSPIP
                     YPRNAEKARYLKHVGIYAYRRDVLQNYSQLPESMQEQAESLEQLRLMSAGINIRTFEV
                     AATGPGVDTPACLEKVRALMAQELAENA"
     gene            complement(4086..5762)
                     /locus_tag="ACHKFK_003936"
     CDS             complement(4086..5762)
                     /locus_tag="ACHKFK_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HFO9054174.1"
                     /translation="MKLFKSILLIAACHAAQASAAIDINADPNLTGAAPLTGILNGQQ
                     SDTQNMSGFDNTPPPSPPVVMSRMFGAQLFNGTSADSGATVGFNPDYILNPGDSIQVR
                     LWGAFTFDGALQVDPKGNIFLPNVGPVKVAGVSNSQLNALVTSKVKEVYQSNVNVYAS
                     LLQAQPVKVYVTGFVRNPGLYGGVTSDSLLNYLIKAGGVDPERGSYVDIVVKRGNRVR
                     SNVNLYDFLLNGKLGLSQFADGDTIIVGPRQHTFSVQGDVFNSYDFEFRESSIPVTEA
                     LSWARPKPGATHITIMRKQGLQKRSEYYPISSAPGRMLQNGDTLIVSTDRYAGTIQVR
                     VEGAHSGEHAMVLPYGSTMRAVLEKVRPNSMSQMNAVQLYRPSVAQRQKEMLNLSLQK
                     LEEASLSAQSSTKEEASLRMQEAQLISRFVAKARTVVPKGEVILNESNIDSVLLEDGD
                     VINIPEKTSLVMVHGEVLFPNAVSWQKGMTTEDYIEKCGGLTQKSGNARIIVIRQNGA
                     AVNAEDVDSLKPGDEIMVLPKYESKNIEVTRGISTILYQLAVGAKVILSL"
     gene            complement(5786..6934)
                     /locus_tag="ACHKFK_003937"
     CDS             complement(5786..6934)
                     /locus_tag="ACHKFK_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFO9054175.1"
                     /translation="MLIKVKSAVSWMRARLSAISLADIQKHLAKIIILAPMAVLLIYL
                     AIFSQPRYMSESKVAIKRSDDLNSGSLNFGLLLGASNPSSAEDALYLKEYINSPDMLA
                     ALDKQLNFREAFSHSGLDFLNHLSKDETAEGFLKYYKDRINVSYDDKTGLLNIQTQGF
                     SPEFALKFNQTVLKESERFINEMSHRIARDQLAFAETEMEKARQRLDASKAELLSYQD
                     NNNVLDPQAQAQAASTLVNTLMGQKIQMEADLRNLLTYLREDAPQVVSARNAIQSLQA
                     QIDEEQSKITAPQGDKLNRMAVDFEEIKSKVEFNTELYKLTLTSIEKTRVEAARKLKV
                     LSVISSPQLPQESSFPNIPYLIACWLLVCCLLFGTLKLLLAVIEDHRD"
     gene            complement(7006..7989)
                     /locus_tag="ACHKFK_003938"
     CDS             complement(7006..7989)
                     /locus_tag="ACHKFK_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFO9054176.1"
                     /translation="MSERHLPDDQSSTIDPYLITSVRQTLAEQGAALQNLSKQLDSGQ
                     YQRVLNLIMNCKGHVILSGMGKSGHVGRKMSATLASTGTPSFFIHPAEAFHGDLGMIT
                     PYDLLILISASGETDEILKLVPSLKNFGNRIIAITNNGNSTLAKNADAVLELHMANET
                     CPNNLAPTTSTTLTMAIGDALAIAMIRQRKFMPNDFARYHPGGSLGRRLLTRVADVMQ
                     HDVPAVQLDASFKTVIQRITSGCQGMVMVEDAEGGLAGIITDGDLRRFMEKEDSLTSA
                     TAAQMMTREPLTLPEDTMIIEAEEKMQKHRVSTLLVTNKANKVTGLVRIFD"
     gene            complement(8799..8969)
                     /locus_tag="ACHKFK_003939"
                     /pseudo
     CDS             complement(8799..8969)
                     /locus_tag="ACHKFK_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082154.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            9325..10203
                     /locus_tag="ACHKFK_003940"
     CDS             9325..10203
                     /locus_tag="ACHKFK_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9054177.1"
                     /translation="MKILPVVSTKGGEGKSTQSANLAGFLADAGIRTLLIDGDHAQPT
                     ASNIFPLTYEAPAGLFELLMRTADLTHPDNIISRTAIDGLDLIVSNDPHEQLKTAMLH
                     APDGRLRLRNVLQHPLFLNNYDVIVVDSQGARSVMLEMIVLSATESVVGMVNPVLPDV
                     REFIRGTVNVMENLLPYRELGIPLPKVRTLVNCMDYTALARKTLDELAGIIQTGRYSR
                     QLPEGAVSLLSTQIYDLNIYKQGHAAGQPVHRLEKESTRRSDSALVTMHSLACELFPE
                     WKPAFDGLLENGGIRQ"
     gene            complement(10634..11107)
                     /locus_tag="ACHKFK_003941"
     CDS             complement(10634..11107)
                     /locus_tag="ACHKFK_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054178.1"
                     /translation="MNTEKQLPSGIDNNHDEDGNMAKYYENREAGGMSTDDVERCKTF
                     FDQGTEIFPGADQKLAYRLCWPGHWLINSRRWFFLDDNSISIQQAVKFALIHQHHWLP
                     ATVEQMTPAEMSLALTDYWADYWTQPTEPEKALVHMTDWQYQKLCSNRKKVNGDQ"
     gene            11334..12182
                     /locus_tag="ACHKFK_003942"
     CDS             11334..12182
                     /locus_tag="ACHKFK_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054179.1"
                     /translation="MSSSVKYSPVPAVLTRPELLAELNLLMTRIASGCRVGVPASCRP
                     VIAVHGGYVSYSSPRAGRVFQDGSHPEIVMAVSNRERMCEWFSQVWLPLHLAALEGDE
                     SAFVSAEVNMVRRELQRVPGSSVNCMCWASLAALAFDAEERVELQKGNNENIATYDES
                     IATYAFRAACTVSLCLSRNVSGGIPRSYPEWKAILFPRDGADIFPDGWLENMTRRAER
                     VFTSAKRQCASEQCSGRIQADSHHSGSHPDVSAYEVRCAQEMSLSPVHSTRQCSVQST
                     DSAETE"
     gene            12530..>13037
                     /locus_tag="ACHKFK_003943"
     CDS             12530..>13037
                     /locus_tag="ACHKFK_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HFO9054180.1"
                     /translation="MAERYIPRSVDAEQAVLGGLMLDNDRWDEVVLMLRGEDFSLAVH
                     RVIFRSMSELAVSGQPFDLITLSEHIEKTHNIEALGGFAYLAELSKNTPSAANIMAYV
                     RIVAEKSMLRQLQETGNSLVADVASPDATPRAVLESAERRLFSLSQTGMLHERSEVSL
                     SSAMELALR"
BASE COUNT         3159 a         3222 c         3183 g         3473 t
ORIGIN      
        1 gttacttttc tttgaagagg atggaaatga ttttttggct acttaaaatt caaaagatat
       61 tgacttgaaa tttaatatat attatgttgg cagtttgttg tgttcccccc ataataaacc
      121 caattaacct gcgtcttcac caataaatac ccacgaaact tcttaaacag tttcatatct
      181 ggtttaaaat cagcctgcca gaactggtgc aaatgccctt gatacgtcaa gcctttgatg
      241 tcgtacaggg cattgcccat cactttgagt ggtttgttat gaatcagtgc agagatcccc
      301 gccgtactgt taatcgtcac caccgctttt gcgtggcgta ataattccgg catcgggaga
      361 tcgtgcacat aaatgactcg ctcacctaag ccatattcct tacttagccg cttaattaat
      421 ggtcgataga gtctgtgacc acgatccatc ggatgatgtt tgatcaccaa ataactttct
      481 ttcggcgctt tacgcgaaaa tgagtacatg acttcattaa tatagtcacg catatcgtta
      541 taattgctgt ggttacgaat ctggctatcg ttatatacct gcaaaacggc aagataataa
      601 cgctggtcca actcgttcat taacctcggt aataccttac gctgtgttac cttgtaaagt
      661 tgcttgcgcc agtatgcacg aacccagcaa cgagcctcat accaggggga aaacgattta
      721 tggtggcggt agcgagggaa ctcatggcgg taatgccagc ccatcaggta ataccacatc
      781 gcatgaccta tacgtttcat cgttgaaggt gttaagttct caacgtgcgg cgtaggcata
      841 tctggtaact tacgataaaa atccggatcg cgcggtagcg atgaatatgc gtttactccg
      901 ccttcttcaa cggtaataaa ttgcgggcgt aaatatcctt cctcaaatgc cagaaagcgg
      961 atccctttcg actttgccca acgttttgct tctttgtgca atgggcggca gtcaccaaag
     1021 cagaggatgg tatcaaagtc atattgccgg tggagatccc gtaaccatcc ggggaactct
     1081 ttcggcgttt ggtagtaagc caggtattgt cgatggcggc agtaaaaacg atccccaccg
     1141 ttgaatacaa cattcacagc gttacgtcct aatgactcta accattcggc gacgtcattg
     1201 aaaaaaggtc ccatcggccc ctgcaatagc agatattttt taccggataa taaaacggtt
     1261 agtgcattac cttgcataaa taatttgcgt aatagtcaat aatgtaattg tttagccaaa
     1321 tctgaccttg cagaacataa ttaatttgcg gtaatagcgt attactcgcc ccgctttttt
     1381 tctggtaata aacatcggct tgcgcggcgt cttgattaaa tattccgcag cttcttctac
     1441 cgtaataggt tgtagccgtg ttgggtggat ataggttgga taaacaatca gcgtctgata
     1501 tagcagatcc gctaacgtta attttcgctc gcgacgcggg cagcgatgtt catcgacggt
     1561 taaaccccaa ccggcataga agggcaggcc gtaacaatgt acgtgcttgc catgtaataa
     1621 tgcttcaaac cccgacagcg acgtcatggt atgcacttca tctgcgcgtt ggatgcattg
     1681 aataatatct gcgtccagtg cctgataatc agcgagttca gcaatctgtt ctgccggaat
     1741 atcgcccttg cgattcccca ctaatacatc cgggtgcggt ttataaataa tgtaggcgtg
     1801 cggattgcgc tcgcgtactg tgcgtaataa ctcaaggttg ctcttaatcg acacagtgcc
     1861 tgttttaata gaggcatcgt cctctacctg acccggcacc aggataactt ttttatcttt
     1921 ggcttcggct ggtagagaga aatcggctcc caggttgtac ttactcagtt tgctttcaac
     1981 cagtcgctgg cgtaattttt cagctcgcat cttctgcgcc agcgttagct ggttatgatt
     2041 cagcagcact tccagatcgc tggggcgcgt ggcgtcatag taaataccgc gtttatccag
     2101 caccagcgat agcggcggca gcaggtcaga gccaagtccg gatgaacgca gaaaaccatc
     2161 ttccattcgc cataacggca gtgattttcg ctgcgcttcg gctcgccatt gctgttctcc
     2221 ctttacaccc cataccacgc aggcgctcgc cgcagtacag cgacgtgaaa aactcagccg
     2281 gtttgttgcc gtttgcaaga aaggtttcag gatcgctgac ttccacagcg ttaagcctgg
     2341 cacccataaa taaccattac gctgttgcag atgtcgacgt tgtaattgca gccattgcag
     2401 cacggtaaat agattgctta cttctcccgt ttgcggatcg atatagcgac agtaacgcag
     2461 gtatgcagcg gcaaaaagtt cctccagcgt ggcagaaccg cgtcgggcag ataacaaagc
     2521 ggactgcgga tggcgatcgt cggttaagcc ccagcctgca taccagggct ggccgaaaca
     2581 tgttactggt tttcctgcca gcaaggcttc aaagccgtat tgggatgtca cgacgtaaac
     2641 ccgggaaacg tgtcgcaaca gcgactgcgg gctgacattc tcggcaatca aacgtactcg
     2701 ttgcgtggcg cgcagatcgg cgaaataacc tgttttcttt ccttccagga catccggatg
     2761 caccttcacc caaatttcgg cttgaggatt ttccgccatc gcggcttcca gcatggcagc
     2821 aaactcatgc gggccagcat tgccatacgt cactgacata tcattaaatg tctgatcgac
     2881 aaccagaacg atgtctgaac gttctgactc atcagctaca aacgcaggtg ccaggttata
     2941 tttcgacaaa tccccggtca cgatggtgtg catcgcttct ctggcctgat cagccaatgc
     3001 cgcatttccg gctttatcct gtaccagttt ctccagcgcc gaaggcttgc tggcatcgta
     3061 gtaaatgcca caatcatcca ccaccagaga aagcggcggc tcgccattga cgccaagatc
     3121 cagcgaacgc acaaatccat cttccagacg aatgacgggt tttcccgctg ctttggcgat
     3181 ggcgactggt ttcgccgcgc tgggacgatg tccccacacg gcgatagcat caacttcttg
     3241 cggaacaggg cgcagcagag aaagtttctg gcacggttgc gccagaaatt tctccagatg
     3301 cggaatacgc cagatgccag gcgagtaaat gccaatcatg cgttttcagc cagttcctgt
     3361 gccatcaggg cgcgcacttt ttccaggcat gccggggtgt cgacgcccgg accggttgcg
     3421 gcaacctcaa atgtacggat gttgatcccc gcgctcatca accgcagctg ctccagtgat
     3481 tctgcctgct cctgcatgga ctccggtaac tggctgtagt tttgcagcac atcgcgacga
     3541 taagcgtaaa taccaacgtg tttcaggtag cgcgcttttt cagcattacg cggatacgga
     3601 ataggcgaac ggctgaaata aagcgcatcc tggtgggtat ttaccaccac ttttaccgtg
     3661 cttggctcgg tcgcttcttc ggcagaaatc gcgtggcata gcgttgccac tggcaacgcg
     3721 gggtcatcac gcattccttg taacagcgtt tctacatccc gcgggcgaat cattggttcg
     3781 tcgccctgca ggttaatgta gatatctgcc tcgactttat gcatcacctc gaccagccga
     3841 tcggtgccgg attcatgatc gttgcgcgtc atgatggctt tcccgccaaa tgcctgcacg
     3901 gccttttcaa cacgctgatc gtctgttgcc acccaaactt ccgcaacgcc cgccacctgt
     3961 aacgcacgct cgtaaacatg ctggatcatc ggtttaccaa caatatcgag cagtggctta
     4021 cccggcaggc gcgatgagcc ataacgagcc ggaatgacaa taactgcttt gctcatttca
     4081 actccttaca aagacagaat cacttttgca cccaccgcca gctgatagag gatggtggaa
     4141 ataccacggg taacttcaat gtttttcgat tcatatttcg gcagaaccat aatctcatcg
     4201 cccggtttga gtgaatccac atcttctgcg ttgacggctg caccgttctg acgaatgacg
     4261 ataattctgg cgttacccga tttctgcgtc aggccaccac atttctcgat gtaatcctcg
     4321 gtggtcatac ccttctgcca gctcaccgcg ttcgggaaca gcacttcgcc atgaaccata
     4381 accagcgatg ttttctccgg aatattgatg acgtcgccat cttcaagcag aacagaatca
     4441 atattggatt cgttgaggat cacttcacct ttcggaacaa cggtgcgtgc tttcgccaca
     4501 aagcggctga tcagttgcgc ttcctgcatt cgcaggctgg cttcttcttt ggtggaggac
     4561 tgggcggaaa gtgatgcttc ctccagcttt tgcagcgaga gattcagcat ctctttctga
     4621 cgctgagcca ctgatgggcg ataaagctga actgcgttca tctgcgacat gctgttcggg
     4681 cggacttttt ccagaaccgc acgcatagtg gaaccataag gcagtaccat ggcatgttca
     4741 ccggagtgtg cgccttcaac ccgcacctga atggtgccgg catagcggtc agtgctcacg
     4801 attaaggtat cgccattttg caacatacgg cctggcgcag aactgatcgg atagtattcg
     4861 ctgcgttttt gcagcccctg tttacgcata atcgtaatgt gagtcgcgcc aggcttaggg
     4921 cgcgcccagc tcaacgcttc cgttacggga atgctgcttt cgcggaactc aaagtcgtag
     4981 ctgttaaaga catcgccctg aacgctgaaa gtatgctgac gcggcccgac gatgatggtg
     5041 tcaccatcgg cgaactgcga aagccccagt ttgccgttca gcaggaagtc gtacaggttg
     5101 acgttggagc gcacgcggtt accgcgcttg accacaatat caacgtaact tccgcgctct
     5161 ggatcaacgc cgccagcctt gatcagataa ttgagtaacg aatcagacgt cacgccgcca
     5221 tacagaccag gattacgcac aaatccggtc acgtacactt ttactggctg cgcctgtaat
     5281 aaggaggcgt agacgttgac gttggactgg tatacttcct tcactttgga tgtgaccagg
     5341 gcatttagct gactattact gacgccagca actttcaccg gaccaacgtt cggcaggaaa
     5401 atattacctt tgggatcaac ctgtaacgca ccatcaaagg tgaacgcacc ccacaagcga
     5461 acctgaatgc tatcacccgg attcagaata tagtcagggt tgaatcctac cgtcgcaccg
     5521 ctatccgcgc tggtgccgtt gaaaagttga gcaccaaaca tacggctcat taccaccggc
     5581 ggtgagggcg gcggggtatt gtcgaagccg ctcatgtttt gcgtatccga ctgttgcccg
     5641 ttcagaatac cggtaagcgg cgcggctcct gtaaggtttg ggtcagcgtt aatatcaatg
     5701 gccgcgctgg cctgcgccgc gtgacaggcg gcaatcagta aaattgattt aaataatttc
     5761 atcacaaact cattcagcga cagcgttagt ctcggtgatc ttcaataaca gccagcaaca
     5821 gtttcagggt gccgaacagc aggcagcaca ccagtaacca gcaggcgatc aaataaggga
     5881 tatttggaaa agacgattcc tgcggcaact gtggcgaact gatcactgac agcaccttga
     5941 gcttacgagc cgcttctaca cgagtctttt caatggaggt cagggtcagt ttgtacagct
     6001 cggtgttgaa ctctactttt gatttgattt cttcaaaatc cactgccata cggtttagct
     6061 tgtcaccctg tggcgcagtg attttgcttt gttcttcatc gatttgtgcc tgcaatgact
     6121 gaatcgcatt acgcgcactc actacttgcg gggcgtcctc acgcagatac gtcagcaagt
     6181 tacgcagatc cgcttccatc tggatcttct ggcccatcag cgtattgact aacgtgctcg
     6241 ccgcctgtgc ctgtgcctgt ggatccagaa cgttgttgtt gtcctgataa gagagcaatt
     6301 ccgctttgct ggcgtccaga cgctggcgtg ccttttccat ctccgtttct gcaaaggcaa
     6361 gctggtcacg cgcgatgcga tgtgacatct cattgataaa gcgctctgac tctttcagca
     6421 cggtctggtt aaacttaagc gcaaactccg ggctaaagcc ctgcgtctga atattcagta
     6481 atccggtttt atcgtcatac gagacgttga tacggtcctt gtagtacttc aggaatcctt
     6541 ctgcggtttc atccttgcta agatgattaa gaaaatcgag cccgctgtgg ctaaatgctt
     6601 cacgaaaatt tagttgctta tccagcgccg ccagcatatc cggcgagttg atgtactctt
     6661 tcagatacaa agcatcttct gcggaactgg ggttagaggc acccagaagc agaccaaaat
     6721 tcaggctgcc gctgtttaaa tcatccgagc gtttaatggc gactttcgac tcgctcatat
     6781 agcgaggctg gctgaagata gccagataga tcagcagcac cgccatcggt gccagaatga
     6841 tgattttcgc caggtgtttt tggatatctg ccagtgagat ggcagacaga cgagcacgca
     6901 tccaggatac ggcagacttc acttttatca acatgtaacg attatcaaag tttcagtggg
     6961 gcgcaaactc aatgagagac taacctctcg aaccccgttg cttaattagt cgaaaatgcg
     7021 cacaaggcca gtgactttat ttgccttgtt ggtcaccaat aacgttgaga cacggtgttt
     7081 ttgcattttt tcttccgctt caatgatcat ggtgtcttcc ggtagcgtca gcggttcacg
     7141 tgtcatcatc tgcgcagccg tggcggatgt cagagaatcc tctttttcca taaagcgacg
     7201 caggtcaccg tcggtgataa tgcccgctag cccaccttct gcgtcttcta ccatcaccat
     7261 tccctggcat ccgctggtga tacgttgaat cacggtttta aatgacgcat ccagctgtac
     7321 cgcaggaaca tcatgctgca tgacatcagc aacgcgggtc agcaggcgac gacctaatga
     7381 accgcccggg tgatagcgcg caaaatcatt cggcataaat ttgcgttggc ggatcatggc
     7441 aatcgccagc gcatcgccga tcgccatcgt cagcgtggta gacgttgttg gtgcaagatt
     7501 attcgggcag gtttcattcg ccatatggag ttccagcacg gcatcagcat ttttcgccag
     7561 cgtggaattt ccattattgg taatggcgat aattcggttg ccgaaatttt tcagtgaagg
     7621 aactagcttg aggatttcat ccgtttcacc gctggcagaa ataaggatca gaagatcgta
     7681 aggcgtaatc atgcccagat cgccatggaa agcttctgca ggatgaataa agaaactagg
     7741 cgtacccgta gaggccagcg tcgctgacat tttacgaccg acatgccctg atttacccat
     7801 tcccgaaaga ataacgtgcc ctttacagtt cattatcaaa ttaaggacac gctggtactg
     7861 cccggaatcc agttgtttag acaagttttg taatgctgcg ccttgttctg ccagagtctg
     7921 gcgaacagag gtaattagat atggatcgat agtactgctc tggtcatcag gtaaatgtct
     7981 ttcagacata tcagctcctt tgcacggaaa taatgccaca gtcacaacaa acccgaaccc
     8041 actataattt atttatgcta aatcaatggg ttgtaacaaa atttttaatg aatataaaac
     8101 catggtcagt ccatgcttat atgcaggact tatcaccttc ttattaagtc gcaatgtctc
     8161 atggaggtgc gaagatgctg caatattaca ggcgagaagg ggaaccaaat tttgcccacc
     8221 tatttaacac acccctacat tatgaacaag gaatttagga tttttatcat ttattaaaat
     8281 gtttctcact tgactattaa gaccaaaaac aatttatcaa ttgattattt cctcgtaaaa
     8341 aaagaacgta tgaccatgtt attaataaat aaaacacgta aataatggtg atgttgtttt
     8401 gtgattaact atttaaagcg gcataataaa cagattgaca atttctatgt aaagaagagg
     8461 aaaataataa aattttagcg gtgactattg aatgatatga tagttgtgat gttgattatt
     8521 tctggtaatg aatgcggaat taaatctggc cggattgtat ttttgctata tctgtttttc
     8581 aggagattat tattaaatag aaaaataaca gtggtgatta tctccggttg agctggagtc
     8641 acgtatgcca gcgagtgctt tcttccgggc cttccttcac ttcagataaa gagcctatgg
     8701 aaaagaggca atcatcaggc tctttttgag tcatatgcgc cgataatcaa ctgtcttgaa
     8761 cgtaaaaatt atggagcaac atgatgctac gattcatttc actcttcgtt ctttctgagc
     8821 attgcgttca ggatggtcag caatttacgc atacagtcaa ccagcgccac ttttttggtt
     8881 tttccggctg ctgacagatg catgtaaaat gcctttatca ccggattaaa ccaggttacc
     8941 acaagcgctg ccatgtgcag ggctgctctt gcctccgtac gcgcactggc gtcgttcggc
     9001 cagcagatat gtgtgggatt acagggatgt tatgtgaact gaactgtgtt ttgttaacgg
     9061 gggatgttta ttgatgattc gggagtgttt aagacctgat ggcgttatgc tcccaagttt
     9121 attttatata aagatccaaa agcaaaaacc cgccttgtgg gcgggttctt tagaatagtg
     9181 gtgcccggac tcggaatcga accaaggaca cggggatttt caatcccctt gtcataaaat
     9241 tcagatagtt acgggtatgt caagttgtcg ttgaagtctt tcatgtgcat ccgatcgcaa
     9301 aaaacagtcg atgaggacgc agatatgaaa atacttcccg ttgtttcaac aaaaggtggt
     9361 gaaggtaaat caacacaatc cgcaaatctt gctggttttc tggccgatgc aggtatacgt
     9421 accctgctga ttgacggaga tcatgctcaa cccacagcca gtaatatctt tccgctgaca
     9481 tatgaagcgc cagccggcct ttttgaactc cttatgcgta cggctgacct cactcatccc
     9541 gataatatca tttcccgcac ggctatcgat ggccttgatc tgattgtctc caatgatcct
     9601 catgaacagt taaaaaccgc gatgcttcac gccccggacg ggcgtcttcg cctccggaat
     9661 gttctgcaac atccactttt cctgaataac tacgatgtca ttgtcgttga ttctcagggg
     9721 gcgcgctccg tcatgctgga gatgatagtg ctgtcggcga ctgagtcggt tgtcgggatg
     9781 gtcaatccgg ttcttcccga tgttcgtgaa tttattcgcg gtactgtcaa cgtcatggag
     9841 aacctgctgc cctaccgtga acttgggata cccctgccta aagtacgcac gctggttaac
     9901 tgtatggact ataccgcgct ggccaggaag acgctggatg agctggccgg aattattcag
     9961 accggtcgtt acagcagaca gttacctgaa ggtgccgttt cccttctttc cacacagatt
    10021 tacgacctga atatttacaa gcagggacat gcggctggtc aacccgttca tcgtctcgaa
    10081 aaagaatcca cacgtcgcag tgattctgcg cttgtcacga tgcacagcct ggcctgtgaa
    10141 ctctttcctg agtggaaacc ggcgtttgac gggctgctgg aaaacggagg aattcgtcag
    10201 tgattcaggt taagcgagaa ccagtcagct ctgttgctgt ggcttatgga ggttatcaat
    10261 accggaagtc gttatttcat cagtgtcatt aattttatcc atttaatcaa tatgatgaga
    10321 aggattaacc ccatgaataa cagcagcaat accacaaacc gtaatacgtc ttcacccgtt
    10381 gagtcagcct gtaatgcgtt actggatttt attgcccggg aagtctttga gaataaagca
    10441 tcggaagagg atgtcaggga gcgttgcaaa gtatttacag cgctgcttaa cgaaggattt
    10501 aaagccctgc ttgatgagtc agggctgaat ctgtcgaatc ttcgtaaaca gtgtaaggaa
    10561 ctggaatctc agataaccag actggattct cagtccgacg caatttgtcg cagaagcgct
    10621 caaattgaac gcttcattgg tcaccattta ccttttttcg atttgaacaa agtttctggt
    10681 actgccagtc agtcatgtga accagcgcct tctccggctc agtcggctgt gtccagtagt
    10741 cagcccagta atctgtcaga gcaagggaca tctctgcggg tgtcatctgc tcaaccgttg
    10801 ccgggagcca gtgatgctga tgaataagag caaacttaac ggcttgttga atgctgatac
    10861 tgttatcgtc cagaaaaaac cagcgacggc tgttaatcag ccagtgcccg ggccagcaaa
    10921 gtcggtaagc gagcttctgg tcagccccgg gaaagatttc agttccctgg tcaaagaagg
    10981 ttttacaacg ttccacatcg tcggtgctca tgcctccggc ttcccggttt tcataatatt
    11041 ttgccatgtt gccatcctca tcatggttgt tatcaatgcc ggacggcagt tgtttttctg
    11101 tattcataaa aattctcctt taaaacaatc tgttatgagt atgcgaatgc tgtccgtacc
    11161 ttctgaccgg ggtcgccaaa ctcaccggtc ggaaaatgta caggcagtta tgggcatttt
    11221 atgccctgca actgttcgtc cggcgccctt tttccttttt tacacagcca ggtttccggt
    11281 tatctgcggg gaagtgacgc acctggcggg cagagagata tggaggtgaa agtatgtctt
    11341 cctcagtgaa atattctccg gttccggcgg tgttaacccg ccctgaattg ctggctgaac
    11401 tgaacctgct gatgacccgg attgccagtg ggtgtcgtgt gggggttccg gcgtcatgtc
    11461 gtccggtcat tgcggtgcac ggtggttatg tttcatacag ttctccacgc gctggacgtg
    11521 tttttcagga cggttcccac ccggagatag tgatggccgt cagtaaccgg gaacggatgt
    11581 gcgaatggtt tagtcaggtc tggcttccgc tgcatctggc tgcccttgaa ggcgatgaaa
    11641 gtgcttttgt cagtgcggag gtcaacatgg tgcgccggga gctgcagcgc gttccgggga
    11701 gttcagtgaa ctgtatgtgc tgggcaagcc ttgctgcact ggcatttgat gctgaagagc
    11761 gggtggaact gcagaaaggc aataatgaga acatcgcaac atatgatgag agcattgcaa
    11821 catatgcatt ccgtgctgcc tgtactgtga gtctctgtct gagtcggaat gtttccggag
    11881 gtattccgcg ctcataccct gaatggaaag cgattttgtt tccccgtgat ggtgctgaca
    11941 ttttccccga cgggtggctg gaaaatatga cccgtcgggc tgaacgtgtc tttacgagtg
    12001 cgaaacgtca gtgtgccagt gagcaatgct ccggccgtat tcaggccgac agccatcatt
    12061 cagggagcca ccctgacgtt tcagcttatg aagtacgctg tgcacaagaa atgtcgcttt
    12121 caccggtcca ttcgacccga cagtgttcag ttcaatccac tgattctgct gaaacggagt
    12181 gaaaggatga gccgattcaa tacagttcgg ggggaagggg ttacagagcc cgtctttttc
    12241 cgtcatgacc tttccgttac tcagcagcca gaactgcagg gtgtatttca cttcagatcc
    12301 aatgccggac ggcagttgtt tttctgtatt cataaagttt ttcctttaaa tcagattgtt
    12361 atgaatgaac gaatgctgtc cgtaccttct gaccggggtc ggcaaactta ccggtctgaa
    12421 aacgtacagg cagttatggg cattttatgc cctgcaactg ttcgtccggc accctttttc
    12481 cttttttcac atagccgggt ttccggcagt cagagaggga ggatacacca tggctgaacg
    12541 ctatattccg cgctccgttg atgctgaaca ggctgtgctt ggcggcctga tgcttgataa
    12601 tgatcgctgg gatgaagtgg tgctgatgct caggggggag gatttttctc tggctgtgca
    12661 ccgggtgata ttccgctcta tgtcagagct ggcagtcagc ggtcagccgt tcgacctcat
    12721 cacgctgagt gagcatattg agaaaacgca caatattgag gcactgggcg gcttcgctta
    12781 cctggctgag ctcagtaaga atacaccttc agcagccaat atcatggctt atgtccgtat
    12841 tgtggcggaa aagagtatgc tgcggcagct tcaggaaacc ggtaattctc tggttgctga
    12901 tgttgcatcg ccggatgcca ctccgcgcgc agttcttgaa tccgcggaac ggcgactttt
    12961 ttctctttcc cagacaggca tgctgcatga gcgcagtgaa gtgagccttt cctccgcaat
    13021 ggagctggcc ctgcgtc
//
LOCUS       DAZGJT010000115        13034 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZGJT010000115 DAZGJT010000000
VERSION     DAZGJT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13034
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.115"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(148..582)
                     /gene="merR"
                     /locus_tag="ACHKFK_003944"
     CDS             complement(148..582)
                     /gene="merR"
                     /locus_tag="ACHKFK_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hg(II)-responsive transcriptional regulator"
                     /protein_id="HFO9054181.1"
                     /translation="MENNLENLTIGVFAKAAGVNVETIRFYQRKGLLREPDKPYGSIR
                     RYGEADVVRVKFVKSAQRLGFSLDEIAELLRLDDGTHCEEASSLAEHKLKDVREKMAD
                     LARMETVLSELVCACHARKGNVSCPLIASLQGEAGLARSAMP"
     gene            654..1004
                     /locus_tag="ACHKFK_003945"
     CDS             654..1004
                     /locus_tag="ACHKFK_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011111453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric transport protein MerT"
                     /protein_id="HFO9054182.1"
                     /translation="MSEPQNGRGALFAGGLAAILASTCCLGPLVLVALGFSGAWIGNL
                     TVLEPYRPLFIGAALVALFFAWKRIYRPVQACKPGEVCAIPQVRATYKLIFWIVAVLV
                     LVALGFPYVVPFFY"
     gene            1018..1293
                     /gene="merP"
                     /locus_tag="ACHKFK_003946"
     CDS             1018..1293
                     /gene="merP"
                     /locus_tag="ACHKFK_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance system periplasmic binding
                     protein MerP"
                     /protein_id="HFO9054183.1"
                     /translation="MKKLFASLALAAAVAPVWAATQTVTLAVPGMTCAACPITVKKAL
                     SKVEGVSKVDVGFEKREAVVTFDDTKASVQKLTKATADAGYPSSVKQ"
     gene            1329..1751
                     /gene="merC"
                     /locus_tag="ACHKFK_003947"
     CDS             1329..1751
                     /gene="merC"
                     /locus_tag="ACHKFK_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organomercurial transporter MerC"
                     /protein_id="HFO9054184.1"
                     /translation="MGLMTRIADKTGALGSVVSAMGCAACFPALASFGAAIGLGFLSQ
                     YEGLFISRLLPLFAALAFLANALGWFSHRQWLRSLLGMIGPAIVFAATVWLLGNWWTA
                     NLMYVGLALMIGVSIWDFVSPAHRRCGPDGCELPAKRL"
     gene            1803..3497
                     /gene="merA"
                     /locus_tag="ACHKFK_003948"
     CDS             1803..3497
                     /gene="merA"
                     /locus_tag="ACHKFK_003948"
                     /EC_number="1.16.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016152 - mercury (II) reductase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0045340 - mercury ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA];
                     GO_process: GO:0050787 - detoxification of mercury ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury(II) reductase"
                     /protein_id="HFO9054185.1"
                     /translation="MSTLKITGMTCDSCAVHVKDALEKVPGVQSADVSYAKGSAKLAI
                     EVGTSPDALTAAVAGLGYRATLADAPSVSTPGGLLDKMRDLLGRNDKTGSSGALHIAV
                     IGSGGAAMAAALKAVEQGARVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRESP
                     FDGGIAATTPTIQRTALLAQQQARVDELRHAKYEGILEGNPAITVLHGSARFKDNRNL
                     IVQLNDGGERVVAFDRCLIATGASPAVPPIPGLKDTPYWTSTEALVSETIPKRLAVIG
                     SSVVALELAQAFARLGAKVTILARSTLFFREDPAIGEAVTAAFRMEGIEVREHTQASQ
                     VAYINGEGDGEFVLTTAHGELRADKLLVATGRAPNTRKLALDATGVTLTPQGAIVIDP
                     GMRTSVEHIYAAGDCTDQPQFVYVAAAAGTRAAINMTGGDAALNLTAMPAVVFTDPQV
                     ATVGYSEAEAHHDGIKTDSRTLTLDNVPRALANFDTRGFIKLVVEEGSGRLIGVQAVA
                     PEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFNKDVKQLSCCAG"
     gene            3515..3877
                     /gene="merD"
                     /locus_tag="ACHKFK_003949"
     CDS             3515..3877
                     /gene="merD"
                     /locus_tag="ACHKFK_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0046689 - response to mercury ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mercury resistance co-regulator MerD"
                     /protein_id="HFO9054186.1"
                     /translation="MSAYTVSQLAHNVGVSVHIVRDYLVRGLLRPVACTTGGYGVFDD
                     AALQRLCFVRAAFEAGIGLDALARLCRALDAADGAQAAAQLAVLRQLVERRRAALAHL
                     DAQLASMPAERAHEEALP"
     gene            3874..4110
                     /gene="merE"
                     /locus_tag="ACHKFK_003950"
     CDS             3874..4110
                     /gene="merE"
                     /locus_tag="ACHKFK_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004102820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum mercury transporter MerE"
                     /protein_id="HFO9054187.1"
                     /translation="MNAPDKLPPETRQPVSGYLWGALAVLTCPCHLPILAAVLAGTTA
                     GAFLGEHWGVAALALTGLFVLAVTRLLRAFRGGS"
     gene            4107..4754
                     /locus_tag="ACHKFK_003951"
                     /pseudo
     CDS             4107..4754
                     /locus_tag="ACHKFK_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003132006.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            4889..6568
                     /locus_tag="ACHKFK_003952"
     CDS             4889..6568
                     /locus_tag="ACHKFK_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013124600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9054188.1"
                     /translation="MATDTPRIPEQGVATLPDEAWERARRRAEIISPLAQSETVGHEA
                     ADMAAQALGLSRRQVYVLIRRARQGSGLVTDLVPGQSGGGKGKGRLPEPVERVIHELL
                     QKRFLTKQKRSLAAFHREVTQVCKAQKLRVPARNTVALRIASLDPRKVIRRREGQDAA
                     RDLQGVGGEPPAVTAPLEQVQIDHTVIDLIVVDDRDRQPIGRPYLTLAIDVFTRCVLG
                     MVVTLEAPSAVSVGLCLVHVACDKRPWLEGLNVEMDWQMSGKPLLLYLDNAAEFKSEA
                     LRRGCEQHGIRLDYRPLGQPHYGGIVERIIGTAMQMIHDELPGTTFSNPDQRGDYDSE
                     NKAALTLRELERWLTLAVGTYHGSVHNGLLQPPAARWAEAVARVGVPAVVTRATSFLV
                     DFLPILRRTLTRTGFVIDHIHYYADALKPWIARRERWPSFLIRRDPRDISRIWVLEPE
                     GQHYLEIPYRTLSHPAVTLWEQRQALAKLRQQGREQVDESALFRMIGQMREIVTSAQK
                     ATRKARRDADRRQHLKTSARPDKPVPPDTDIADPQADNLPPAKPFDQIEEW"
     gene            6571..7023
                     /locus_tag="ACHKFK_003953"
                     /pseudo
     CDS             6571..7023
                     /locus_tag="ACHKFK_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003155746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
     gene            complement(7129..7628)
                     /locus_tag="ACHKFK_003954"
                     /pseudo
     CDS             complement(7129..7628)
                     /locus_tag="ACHKFK_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(7756..8595)
                     /gene="sul1"
                     /locus_tag="ACHKFK_003955"
     CDS             complement(7756..8595)
                     /gene="sul1"
                     /locus_tag="ACHKFK_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO9054189.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            complement(8589..8936)
                     /locus_tag="ACHKFK_003956"
     CDS             complement(8589..8936)
                     /locus_tag="ACHKFK_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO9054190.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            complement(9053..9891)
                     /gene="aadA1"
                     /locus_tag="ACHKFK_003957"
                     /pseudo
     CDS             complement(9053..9891)
                     /gene="aadA1"
                     /locus_tag="ACHKFK_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            complement(9984..10457)
                     /gene="dfrA1"
                     /locus_tag="ACHKFK_003958"
     CDS             complement(9984..10457)
                     /gene="dfrA1"
                     /locus_tag="ACHKFK_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO9054191.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            10614..11627
                     /gene="intI1"
                     /locus_tag="ACHKFK_003959"
     CDS             10614..11627
                     /gene="intI1"
                     /locus_tag="ACHKFK_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO9054192.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            11830..12180
                     /locus_tag="ACHKFK_003960"
                     /pseudo
     CDS             11830..12180
                     /locus_tag="ACHKFK_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            12257..12901
                     /locus_tag="ACHKFK_003961"
                     /pseudo
     CDS             12257..12901
                     /locus_tag="ACHKFK_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
BASE COUNT         2573 a         3931 c         3898 g         2632 t
ORIGIN      
        1 atccccccag gggctgcgcc cctcggccgc gaacggcctc accccaaaaa tggcagcgcc
       61 tacggcgctg actctttcgt tgaccaggcg caagcgcctg gaaatgctca attagggggc
      121 acctcagaaa acggaaaata aagcacgcta aggcatagct gaccttgcca ggcctgcttc
      181 gccctgtagt gacgcgatca acgggcagga aacattcccc tttcgtgcat ggcaggcgca
      241 cacgagttca gacagcacgg tttccatgcg cgccaagtcg gccatcttct cgcgcacgtc
      301 cttgagcttg tgttcggcca ggctgctggc ctcctcgcag tgggtgccat cgtcgagccg
      361 caacagctcg gcaatctcgt ccagactgaa ccccagccgc tgtgccgatt tcacgaattt
      421 cacccgaacc acgtccgcct ccccatagcg gcggatgctg ccgtaaggct tgtccggttc
      481 ccgcaacagg cccttgcgct gatagaagcg gattgtctcc acgttgaccc cggccgcctt
      541 ggcaaaaacg ccaatggtca ggttttccaa attattttcc atatcgcttg actccgtaca
      601 tgagtacgga agtaaggtta cgctatccaa tccaaattca aaagggccaa cgtatgtctg
      661 aaccacaaaa cgggcgcggt gcgctcttcg ccggcgggct ggccgccatt cttgcatcga
      721 cctgctgcct ggggccgcta gtactggtcg ccctgggctt ctccggtgct tggatcggca
      781 acctgacggt gctggaaccc tatcgaccgt tgttcatcgg cgcggcgcta gtggcgctgt
      841 tcttcgcctg gaagcggatt taccggcccg tgcaggcatg caagccaggt gaggtctgcg
      901 cgattccgca ggtgcgcgcc acctacaagc tgattttctg gatcgtggcc gtgctggtcc
      961 tggtcgcgct tggatttccc tatgtcgttc catttttcta ttaaccagga gttcatcatg
     1021 aagaaactgt ttgcctccct tgccctcgcc gccgctgttg ccccggtgtg ggccgctacc
     1081 cagaccgtca cgctagcggt tcccggcatg acttgcgccg cctgcccgat cacagtcaag
     1141 aaagcgctct ccaaggtcga aggcgtgagc aaggtcgatg tgggcttcga gaagcgcgag
     1201 gccgtcgtca cttttgacga caccaaggcc agcgtacaga agctgaccaa ggccaccgca
     1261 gacgccggct atccgtccag cgtcaagcag tgagccagca agccaacgac aacagcgaga
     1321 gccgcttcat gggactgatg acacgcattg ccgataaaac cggcgcgctc ggcagcgtcg
     1381 tttccgcgat gggctgcgcc gcctgctttc cagccctcgc cagcttcggc gcggccatcg
     1441 ggctgggctt cttgagccag tacgagggac tgttcatcag ccgcctgctg ccgctgtttg
     1501 ccgcgctggc cttcctggcg aacgcgctgg gttggttcag tcatcggcaa tggctgcgca
     1561 gtctgctcgg catgatcggc ccggccatcg tgtttgcggc cacggtctgg ctgctcggca
     1621 actggtggac ggcgaacctg atgtacgtcg gcctggcctt gatgattggg gtgtcgatct
     1681 gggacttcgt gtcgccggcg catcgccgtt gcggaccgga cggctgcgaa ctccccgcca
     1741 agcgcttgtg aaagacggct gaccgtgcga cacggcggcc cacacgaata aggaacgatg
     1801 gtatgagcac tctcaaaatc accggcatga cttgcgactc gtgcgcagtg catgtcaagg
     1861 acgccctgga gaaagtgccc ggcgtgcaat cagcggatgt ctcctacgcc aagggcagcg
     1921 ccaagctcgc cattgaggtc ggcacgtcac ccgacgcgct gacggccgct gtagctggac
     1981 tcggttatcg ggccacgctg gccgatgccc cctcagtttc gacgccgggc ggattgctcg
     2041 acaagatgcg cgatctgctg ggcagaaacg acaagacggg tagcagcggc gcattgcata
     2101 tcgccgtcat cggcagcggc ggggccgcga tggcagcggc gctgaaggcc gtcgagcaag
     2161 gcgcacgtgt cacgctgatc gagcgcggca ccatcggcgg cacctgcgtc aatgtcggtt
     2221 gtgtgccgtc caagatcatg atccgcgccg cccatatcgc ccatctgcgc cgggaaagcc
     2281 cgttcgatgg cggcatcgcc gctaccacgc cgaccatcca gcgcacggcg ctgctggccc
     2341 agcagcaggc ccgcgtcgat gaactgcgcc acgccaagta cgaaggcatc ttggagggca
     2401 atccggcgat cactgtgctg cacggctccg cccgctttaa ggacaatcgc aacctgatcg
     2461 tgcaactcaa cgacggcggc gagcgcgtgg tggcattcga ccgctgcctg atcgccaccg
     2521 gcgcgagccc ggccgtgccg ccgattcccg gcctgaaaga cactccgtac tggacttcca
     2581 ctgaagcgct ggtcagcgag acgattccta agcgcctggc cgtgattggc tcatcagtgg
     2641 tggcgctgga gctggcgcag gcgttcgccc gactcggagc gaaggtgacg atcctggctc
     2701 gcagcacgct gttcttccgc gaagacccag ctataggcga agccgtcacg gccgcattcc
     2761 gcatggaggg catcgaggtg agggaacaca cccaggccag ccaggtcgcg tatatcaatg
     2821 gtgaagggga cggcgaattc gtgctcacca cggcgcacgg cgaactgcgc gccgacaagc
     2881 tgctggtcgc caccggccgc gcgcccaaca cacgcaagct ggcactggat gcgacgggcg
     2941 tcacgctcac cccgcaaggc gctatcgtca tcgaccccgg catgcgtaca agcgtggaac
     3001 acatctacgc cgcaggcgac tgcaccgacc agccgcagtt cgtctatgtg gcggcagcgg
     3061 ccggcactcg cgccgcgatc aacatgaccg gcggtgacgc ggccctgaac ctgaccgcga
     3121 tgccggccgt ggtgttcacc gacccgcaag tggcgaccgt aggctacagc gaggcggaag
     3181 cgcaccatga cggcatcaaa actgatagtc gcacgctaac gctggacaac gtgccgcgcg
     3241 cgctcgccaa cttcgacacg cgcggcttca tcaaactggt ggttgaagaa ggcagcggac
     3301 gactgatcgg cgtgcaggca gtggccccgg aagcgggcga actgatccag acggccgcac
     3361 tggcgattcg caaccggatg acggtgcagg aactggccga ccagttgttc ccctacctga
     3421 cgatggtcga agggttgaag ctcgcggcgc agaccttcaa caaggatgtg aagcagcttt
     3481 cctgctgcgc cgggtgagga caaggaggtg tgcgatgagc gcctacacgg tatcgcaact
     3541 ggcccataac gttggggtga gcgtacatat cgtgcgcgac tacctggtgc gcggcttgtt
     3601 acggccggtg gcctgcacca cgggcggcta cggcgtgttc gacgatgcgg ccttgcaacg
     3661 gctgtgcttc gtgcgcgcgg ccttcgaggc gggtatcggc ctggatgccc tggcgcggct
     3721 gtgccgtgcg ctcgacgcag cggacggcgc acaagccgca gcgcagcttg ccgtgctgcg
     3781 ccagttggtc gagcggcggc gcgcggcgtt ggcccatctg gacgcgcaac tggcctccat
     3841 gccagccgag cgggcgcacg aggaggcatt gccgtgaacg cccctgacaa actgccgccc
     3901 gagacgcgcc aacccgtttc cggctacctg tggggtgcgc tggccgtgtt gacctgcccc
     3961 tgccatctgc cgattctcgc cgccgtgctg gccgggacga ccgccggtgc cttccttggc
     4021 gagcattggg gtgttgccgc gctcgcgctg accggcttgt tcgttctggc cgtaacgcgg
     4081 ctgctgcgcg ccttccgggg cggatcatga cgagttcgca gcccgccgga tggacggcgg
     4141 ccgagttggc gcaggcggcg gcgcgcggac agcttgacct gcattaccag ccgctggtcg
     4201 atctgcgcga tcaccggatc gctggcgcgg aagcgttgat gcgctggcgg catccgaggc
     4261 ttggcctgtt gccgcccggc cagttcctgc cgctggccga gtcgttcggc ctgatgccgg
     4321 aaataggcgc gtgggtgctg ggcgaggcct gtcgccagat gcacaagtgg caaggaccgg
     4381 catggcaacc gttccgtctt gccatcaatg tgtccgccag ccaggttggg ccaacgttcg
     4441 acgacgaggt aaagcgggtg ctggccgata tggccctgcc cgccgagctt ctggagatcg
     4501 aactgaccga atcggtcgca ttcggcaatc cagccctgtt cgccagtttc gacgccttgc
     4561 gcgccatcgg cgtgcgcttc gccgccgacg acttcggcac cggctattcc tgcctgcaac
     4621 atctgaaatg ctgccccatc accacattga aaatcgacca atcctttgtc gccaggctcc
     4681 cggatgatgc ccgtgaccaa actatcgtgc gggcggtgat ccagctcgcg cacgggctgg
     4741 gcatggatgt cattttcaga agacgactgc accagttgat tgggcgtaat ggctgttgtg
     4801 cagccagctc ctgacagttc aatatcagaa gtgatctgca ccaatctcga ctatgctcaa
     4861 tactcgtgtg caccaaagcg aggtgagcat ggcgacggac accccacgga ttccagaaca
     4921 aggcgtggcc actctgcctg atgaggcttg ggagcgtgcg cgccgtcgtg cggagatcat
     4981 cagtccgttg gcgcagtcgg agacggtcgg gcacgaagcg gccgatatgg cggctcaggc
     5041 gctgggcttg tctcggcgcc aggtatacgt tctgatccgg cgtgcccggc aaggcagcgg
     5101 cctcgtgacg gatctggtgc ccggccagtc cggtggaggt aaaggtaagg ggcgcttgcc
     5161 ggaaccggtc gagcgcgtca tccacgagct actgcaaaag cggttcctga ccaagcagaa
     5221 gcgcagccta gcggcctttc accgcgaagt cactcaggtg tgcaaggctc aaaaactgcg
     5281 agtgccggcg cgcaataccg tggccttacg gatcgctagc cttgacccgc gcaaggtcat
     5341 ccgccggcgg gaaggccagg atgccgctcg tgacctacaa ggtgtgggcg gcgagcctcc
     5401 tgccgtgacc gcgccgctgg agcaggtgca gatagaccat acggtcatcg acctgatcgt
     5461 ggtcgatgac cgcgaccggc aacctattgg ccgcccgtac ctgaccctcg ccatcgacgt
     5521 gttcacccgc tgcgtgctcg gcatggtcgt cacgctggaa gcgccgtctg ccgtttcggt
     5581 tggcctgtgc ctcgtgcatg tcgcctgcga caagcgccct tggctggaag gactgaacgt
     5641 ggaaatggat tggcagatga gcggcaagcc cttgctgctc tacctagaca acgcggccga
     5701 gttcaagagc gaggccctgc gccggggttg cgagcagcat ggcatccggc tggactatcg
     5761 cccgctggga cagccgcact atggcggcat cgtggaacgg atcatcggca cggcgatgca
     5821 gatgattcac gacgaactgc cgggaacgac cttctccaac cctgaccagc gcggcgacta
     5881 cgattccgaa aacaaggccg ccctgacgct gcgcgagcta gagcgctggc tcacattggc
     5941 ggtcggcacc taccacggtt cggtgcacaa cggcctgctc caaccgccgg ccgcgcgctg
     6001 ggccgaggcc gtggcgcgtg tcggcgtacc ggccgtcgtc acacgcgcta cttcgttcct
     6061 ggtcgatttt ctgccgatcc tccggcgcac gctgacccgc accggctttg tcatcgacca
     6121 catccactac tacgccgatg cgctcaagcc gtggattgcg cggcgtgaac gctggccgtc
     6181 ctttctgatc cggcgcgatc cgcgcgacat cagccgtatc tgggtcctgg aaccggaggg
     6241 acagcattac ctggaaattc cctaccgtac cttgtcgcat ccggctgtca ccctctggga
     6301 acaacggcag gcgctggcga aactgcggca gcaagggcgc gaacaggtgg atgagtcggc
     6361 gctgttccgc atgatcggcc agatgcgtga gattgtgacc agcgcgcaga aggccacacg
     6421 caaggcgcgg cgtgacgcgg atcgccgcca gcacctcaag acatcagctc ggccggacaa
     6481 gcccgttccg ccggatacgg atattgccga cccgcaggca gacaacttgc cacccgccaa
     6541 accgttcgac cagattgagg agtggtagcc gtggacgaat atcccatcat cgacctgtcc
     6601 cacctgctgc cggcggccca gggcttggcc cgtcttccgg cggacgagcg catccagcgc
     6661 cttcgcgccg accgctggat cggctatccg cgcgcagtcg aggcgctgaa ccggctggaa
     6721 gccctttatg cgtggccaaa caagcaacgc atgcccaacc tgctgctggt tggcccgacc
     6781 aacaatggca agtcgatgat cgtcgagaag ttccgccgca cccacccggc cagctccgac
     6841 gccgaccagg agcacatccc ggtgttggtc gtgcagatgc cgtccgagcc gtccgtgatc
     6901 cgcttctacg tcgcgctgct cgccgcgatg ggcgcgccgc tgcgcccacg cccacggttg
     6961 ccggaaatgg agcaactggc tctggcactg ctgcgcaagg tcggcgtgcg catgctggtg
     7021 attgtcgaca acctctcgcg caaccaagac atcgcggtcg gactgcaagt gatcttgaag
     7081 ccacgggccc gtcccacccc gacatggacc tcgatgcccg aacggacgtt agatttcgag
     7141 ttctaggcgt tctgcgatga aggttggatc ccagccggga ttgaaagtgt cgacgtgggt
     7201 gaatccgagc cgctcgtata ggccacgcag gttcgggtgg cagtcgagcc gcagcttggc
     7261 gcacccctgc gttcgcgcgg catggcggca agcctcgatc agcgcggagc tgacaccccg
     7321 gcccgcatgt gtccgtcgca ccgcgagctt gtgcagatat gcggcctccc ccttgagggc
     7381 gtcgggccag aactcgggat cctcggccga caaggtgcaa cagccgacga tgccgtcgct
     7441 gcaactcgcg actaggagct cggatctcag gacgaaggtc tccgcgaatg tccggtcgat
     7501 ccgcgcgacg tcccaggcgg cgttcccttg gcggacatcc acgccgcagc gtcgtgcatc
     7561 agccgcacaa cctcgtcgat atcacccgag caggcgaccc gaacgttcgg aggctcctcg
     7621 ctgtccattc gctcccctgg cgcggtatga accgccgcct catagtgcag tttgatcctg
     7681 acgagcccag catgtctgcg cccaccttcg cggaacctga ccagggtccg ctagcgggcg
     7741 gccggaaggt gaatgctagg catgatctaa ccctcggtct ctggcgtcgc gactgcgaaa
     7801 tttcgcgagg gtttccgaga aggtgattgc gcttcgcaga tctccaggcg cgtgggtgcg
     7861 gacgtagtca gcgccattgc cgatcgcgtg aagttccgcc gcaaggctcg ctggacccag
     7921 atcctttaca ggaaggccaa cggtggcgcc caagaaggat ttccgcgaca ccgagaccaa
     7981 tagcggaagc cccaacgccg acttcagctt ttgaaggttc gacagcacgt gcagcgatgt
     8041 ttccggtgcg gggctcaaga aaaatcccat ccccggatcg aggatgagcc ggtcggcagc
     8101 gaccccgctc cgtcgcaagg cggaaacccg cgcctcgaag aaccgcacaa tctcgtcgag
     8161 cgcgtcttcg ggtcgaaggt gaccggtgcg ggtggcgatg ccatcccgct gcgctgagtg
     8221 cataaccacc agcctgcagt ccgcctcagc aatatcggga tagagcgcag ggtcaggaaa
     8281 tccttggata tcgttcaggt agcccacgcc gcgcttgagc gcatagcgct gggtttccgg
     8341 ttggaagctg tcgattgaaa cacggtgcat ctgatcggac agggcgtcta agagcggcgc
     8401 aatacgtctg atctcatcgg ccggcgatac aggcctcgcg tccggatggc tggcggccgg
     8461 tccgacatcc acgacgtctg atccgactcg cagcatttcg atcgccgcgg tgacagcgcc
     8521 ggcggggtct agccgccggc tctcatcgaa gaaggagtcc tcggtgagat tcagaatgcc
     8581 gaacaccgtc accatggcgt cggcctccgc agcgacttcc acgatgggga tcgggcgagc
     8641 aaaaaggcag caattatgag ccccatacct acaaagcccc acgcatcaag cttttgccca
     8701 tgaagcaacc aggcaatggc tgtaattatg acgacgccga gtcccgacca gactgcataa
     8761 gcaacaccga cagggatgga tttcagaacc agagaaagaa aataaaatgc gatgccataa
     8821 ccgattatga caacggcgga aggggcaagc ttagtaaagc cctcgctaga ttttaatgcg
     8881 gatgttgcga ttacttcgcc aactattgcg ataacaagaa aaagccagcc tttcatgata
     8941 tatctcccaa tttgtgtagg gcttattatg cacgcttaaa aataataaaa gcagacttga
     9001 cctgatagtt tggctgtgag caattatgtg cttagtgcat ctaacgcttg agttaagccg
     9061 cgccgcgaag cggcgtcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt
     9121 gatctcgcct ttcacgtagt gaacaaattc ttccaactga tctgcgcgcg aggccaagcg
     9181 atcttcttct tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc
     9241 cggcaggcgc tccattgccc agtcggcagc gacatccttc ggcgcgattc tgccggttac
     9301 tgcgctgtac caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc
     9361 gggcggcgag ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg
     9421 aaccggatca aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc
     9481 ttttgtcagc aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag
     9541 aatgtcattg cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg
     9601 aatgatgtcg tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc
     9661 aggggaagcc gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag
     9721 ccttacggtc accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac
     9781 tgcggagccg tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc
     9841 aactacctct gatagttgag tcgatacttc ggcgatcacc gcttccctca tgatgtttaa
     9901 cgcctggcac agcggatcgc aaacctggcg cggcttttgg tacaaaaggc gtgacaggtt
     9961 tgcgaatccg ttgctgccac ttgttaaccc ttttgccaga tttggtaact ataatttatg
    10021 ttagaggcga agtcttgggt aaaaactggc ctaaaattgc tggggatttc aggaaagtaa
    10081 acatcacctt ccggctcgat gtctattgta gatatatgta gtgtatctac ttgatcgatc
    10141 aggcttttgt atatctcccc accacctgaa acaatgacat gatccgttat tttctttagg
    10201 ttggttaaag catctttaat tgatggaaag atcactacgt tctcattgtc agatgtaaaa
    10261 cttgaacgtg ttacgaccgc atactttcgg ttgggtaatg ctcccattga ttcaaaagtc
    10321 ttgcgtccaa ccaacagcca ttggttatag gtaatagctt taaacaggag ctgttcacct
    10381 ttggcactcc atggaatatc agggccattc ccgataactc cattcttcga tatagctacc
    10441 attagtgata gtttcacaat tcttcctcag aggttaactt tgttttaggg cgactgccct
    10501 gctgcgtaac atcgttgctg ctccataaca tcaaacatcg acccacggcg taacgcgctt
    10561 gctgcttgga tgcccgaggc atagactgta caaaaaaaca gtcataacaa gccatgaaaa
    10621 ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc
    10681 gcatacgcta cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg
    10741 ccttcatccg tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg
    10801 catttctgtc ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat
    10861 tggcggcctt gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg
    10921 agatcggaag acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg
    10981 ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa
    11041 cgggcatgcg gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg
    11101 gcacgatcat cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga
    11161 gcttggcacc cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc
    11221 aggccgaggg ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg
    11281 ccgggcattc ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac
    11341 ggagcggtgt cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac
    11401 gtgccgtaga acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt
    11461 tcgcgacggc cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc
    11521 attccgacgt ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag
    11581 tgcgctcacc gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc
    11641 aatcctggcg gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt
    11701 tgcggaaagt cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga
    11761 tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg
    11821 aaaacgacaa tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg
    11881 cggcaggcgg gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg
    11941 gccttcgtcg gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc
    12001 accagttgct gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gccggaaggg
    12061 gccgagtacg tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc
    12121 agcactgcga ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga
    12181 ggcataccct aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt
    12241 ttgaatttat tgacattcga tcatcaacgt taaatcgggt ggtcgcggac gtttatcatc
    12301 cttcgctgca ggcgtttttt tacttgtgat ggtggtgttt ttaggagagt ggttaaagct
    12361 tattcctatg gcggcactgg ttgccgtgat gatcatggtg gctatcggta cattctcttg
    12421 ggattcaatc cgtaatctta agcatcatcc tatgtcaacc aaccttgtga tggtggcgac
    12481 ggttgtggtc gtggtcgcta cccataactt agcaattggg gtatttgtag gtgtattact
    12541 cgcgtcccta ttttttgcta ataaagtggg acgttttatg gtggttaaga gtacaacgac
    12601 tgctgctgac actggtcgcc attatcaggt tattggacaa gtcttttttg cttcatcaga
    12661 taagttcagt aattcattcg attttaggga agtggttgat aaagtgacta tcgacttatc
    12721 tcaagcacat ttttgggata ttaccgcggt atcggccctc gacaaggtag tgattaaatt
    12781 ccgccgcgag gggactgagg ttgagttgat tggccttaac gaagccagtg ccaccatagt
    12841 cgataaattt ggggtacacg ataagccaga agaagttgaa aaaatgatgt ctggtcacta
    12901 agccattagc aacacaacaa caaaggaata aaacgatgac aaatgtgatt gcttgtattg
    12961 acggttcaaa tgtgacatcc gctgtatgtg atgccagtgg ctgggccgcc tttcaactga
    13021 atgcccctgt aatc
//
LOCUS       DAZGJT010000116        13009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZGJT010000116 DAZGJT010000000
VERSION     DAZGJT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.116"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            272..1330
                     /gene="citC"
                     /locus_tag="ACHKFK_003962"
     CDS             272..1330
                     /gene="citC"
                     /locus_tag="ACHKFK_003962"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO9054193.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1345..1641
                     /gene="citD"
                     /locus_tag="ACHKFK_003963"
     CDS             1345..1641
                     /gene="citD"
                     /locus_tag="ACHKFK_003963"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO9054194.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILEVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1638..2546
                     /gene="citE"
                     /locus_tag="ACHKFK_003964"
     CDS             1638..2546
                     /gene="citE"
                     /locus_tag="ACHKFK_003964"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO9054195.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHTLQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2557..4089
                     /gene="citF"
                     /locus_tag="ACHKFK_003965"
     CDS             2557..4089
                     /gene="citF"
                     /locus_tag="ACHKFK_003965"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO9054196.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYSGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGAAVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4093..4644
                     /gene="citX"
                     /locus_tag="ACHKFK_003966"
     CDS             4093..4644
                     /gene="citX"
                     /locus_tag="ACHKFK_003966"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO9054197.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHAWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4619..5497
                     /gene="citG"
                     /locus_tag="ACHKFK_003967"
     CDS             4619..5497
                     /gene="citG"
                     /locus_tag="ACHKFK_003967"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO9054198.1"
                     /translation="MSMPATSTKNTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQSVTPITICATAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5548..7011
                     /gene="citT"
                     /locus_tag="ACHKFK_003968"
     CDS             5548..7011
                     /gene="citT"
                     /locus_tag="ACHKFK_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO9054199.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7125..7931
                     /gene="rna"
                     /locus_tag="ACHKFK_003969"
     CDS             7125..7931
                     /gene="rna"
                     /locus_tag="ACHKFK_003969"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO9054200.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGNTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8161..8571
                     /gene="rnk"
                     /locus_tag="ACHKFK_003970"
     CDS             8161..8571
                     /gene="rnk"
                     /locus_tag="ACHKFK_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO9054201.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEIPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8714..8803
                     /gene="yldA"
                     /locus_tag="ACHKFK_003971"
     CDS             8714..8803
                     /gene="yldA"
                     /locus_tag="ACHKFK_003971"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO9054202.1"
                     /translation="MSEALGITAFYIFIAAIIVAAVLYLEQRW"
     gene            8927..9355
                     /gene="uspG"
                     /locus_tag="ACHKFK_003972"
     CDS             8927..9355
                     /gene="uspG"
                     /locus_tag="ACHKFK_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO9054203.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSCIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(9477..11042)
                     /gene="ahpF"
                     /locus_tag="ACHKFK_003973"
     CDS             complement(9477..11042)
                     /gene="ahpF"
                     /locus_tag="ACHKFK_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO9054204.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(11213..11776)
                     /gene="ahpC"
                     /locus_tag="ACHKFK_003974"
     CDS             complement(11213..11776)
                     /gene="ahpC"
                     /locus_tag="ACHKFK_003974"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO9054205.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            12148..12894
                     /gene="dsbG"
                     /locus_tag="ACHKFK_003975"
     CDS             12148..12894
                     /gene="dsbG"
                     /locus_tag="ACHKFK_003975"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO9054206.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLSVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
BASE COUNT         3109 a         3421 c         3267 g         3212 t
ORIGIN      
        1 aattaaatca cttaaacctg cgggttattt cgtaattaca ttagttaaaa aacttaaaac
       61 cattaaataa ataaaaaacc actaactcta tgtgaaataa atcaaaattt cacgccgaaa
      121 tactccttag gatgtatagc gaaaggagaa aaagatatac ctcgatcgcc cccctttctc
      181 ccaagtgaaa ataaaaggtt atcaatttgc aacattgaac aacattcgtt gcaaatcgat
      241 aacaacatgc accttcagga tactatttat tatgttcggc aatgatattt tcacccgcgt
      301 aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa ttcctgcatg aaaatgattt
      361 gagcgttgac accacagtcg aagtatttat taccgtaacc cgcgatgaaa agcttatcgc
      421 gtgcggtgga attgccggaa atattattaa atgcgttgct atcagtgaat ccgttcgcgg
      481 tgaaggactg gcgctgacat tagccactga actgataaac ctcgcctatg agcggcatag
      541 cacgcatctg tttatttata ccaaaaccga atacgaggcg ctgttccgcc agtgcggttt
      601 ttccacgctg accagcgtac ccggcgtgat ggtgctgatg gaaaacagcg ccacgcgact
      661 gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca gggaacaaga ttggctgcat
      721 tgtgatgaac gccaatccct ttacgaatgg tcaccgttat ctgattcaac aggctgcggc
      781 acagtgcgac tggttgcatc tgtttttagt caaagaagat tcttcacgct tcccctatga
      841 agaccggctg gatctggtgt taaaaggcac cgccgatatt ccacgcctga ctgtgcatcg
      901 cggctccgaa tacatcatct cccgcgctac gttcccttgc tacttcatta aagaacagag
      961 cgtcattaac cattgttaca ccgaaattga tctgaagatt ttccgtcagt acctcgctcc
     1021 cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc ttttgtcgcg ttactgccca
     1081 gtacaaccag gatatgcgct actggctgga aacaccgact atctccgcac cgcccatcga
     1141 actggttgaa attgagcggc tgcgttacca ggagatgccg atatccgctt cccgggtacg
     1201 tcaactgctg gcaaaaaacg atctcacggc tatcgcgccg ctggtccctg cagtcacgct
     1261 gcattatttg cagaacctgc ttgagcactc ccgccaggac gcggcagctc gtcaaaagac
     1321 ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc ccgccgttgc aggcaccctt
     1381 gagtctgggg atgtgatgat acgcatcgcc ccactcgata cgcaggatat cgacctgcaa
     1441 atcaacagca gcgttgagaa acagtttggc gatgcaattc gcaccaccat tctggaagtt
     1501 ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg atgacaaagg cgcactggac
     1561 tgcattttac gtgcacgact ggaagccctg ctggcacgcg ccagcggtat cccggctctg
     1621 ccatgggagg attgccaatg atttccgctt cgctgcaaca acgtaaaact cgcacccgcc
     1681 gcagtatgtt gtttgtgcct ggtgctaatg ccgcgatggt cagcaactcc ttcatctacc
     1741 cggctgatgc cctgatgttt gacctcgaag actccgttgc attgcgtgaa aaagacaccg
     1801 cccgccgcat ggtttaccac acgctgcaac atccgcttta tcgcgatatt gaaaccattg
     1861 tgcgtgtaaa cgcgctggat tccgaatggg gtgttaacga cctggaagcc gtcgttcgcg
     1921 gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc tcaggatgtt ctggatatcg
     1981 aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga acccggcagt accggcctgc
     2041 tggcggcgat tgaatctccg ctgggcatta cccgcgcggt agaaatcgct cacgcttccg
     2101 agcgtttgat cggtatcgcc ctcggtgcag aagactatgt gcgcaacctg cgtacagaac
     2161 gctccccgga aggaactgaa ctgctgttcg ctcgctgctc cattttgcag gccgcgcgtt
     2221 ctgcgggtat tcaggcgttc gataccgtct attccgacgc taacaacgaa gccggattcc
     2281 tgcaagaagc cgcccacatc aaacagctgg gctttgacgg caaatcgctg atcaacccgc
     2341 gtcagattga tctgctgcac aacctctacg caccgaccca gaaagaagtg gatcacgccc
     2401 gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg cctcggcgtg gtttccctga
     2461 acggcaagat ggtggacggt ccggttatcg atcgcgcccg tctggtgctc tcccgcgcag
     2521 aactttccgg catccgcgaa gaataaggca atcaaaatga cgcagaaaat tgaacaatct
     2581 caacgacaag aacgggtagc ggcctggaat cgtcgcgctg aatgcgatct tgccgctttc
     2641 cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc gcgatcgcaa actgtgcgcc
     2701 aacctggaag aagcgattcg tcgctctggt ttacaggacg gcatgacggt ttccttccat
     2761 cacgctttcc gtggcggtga cctgaccgtc aatatggtga tggacgtcat cgcgaagatg
     2821 ggctttaaaa acctgactct ggcctccagc tccctgagtg attgccatgc gccactggta
     2881 gaacacattc gccagggcgt ggttacccgc atttatacct ctggcctgcg cggtccactg
     2941 gcggaagaaa tctcccgtgg tctgctggcg gaaccggtgc aaatccactc tcacggcgga
     3001 cgtgtgcatc tggtacagag cggcgaactg aatatcgatg tggctttcct cggcgtcccg
     3061 tcctgtgatg aattcggtaa tgccaacggc tactccggta aagcctgctg cggctccctc
     3121 ggctatgcaa tggttgatgc cgacaacgca aaacaggtcg tgatgcttac cgaagaactg
     3181 ctgccttatc cgcataatcc ggcaagcatt gagcaagatc aggttgatct gatcgtcaaa
     3241 gttgatcgcg ttggcgatgc tgcaaaaatc ggcgctggtg ctacccgtat gaccactaac
     3301 ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga ttgttaactc tggctacttc
     3361 aaagaaggtt tctccatgca gaccggcacc ggcggcgctt cgctggcggt aacccgtttc
     3421 ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact tcgccctcgg cggtattacc
     3481 gcaacgatgg ttgatctgca cgaaaaaggt ttgatccgca aactgctgga tgtgcagagc
     3541 tttgacagcc atgctgcgca atcgctggcc cgtaacccca atcacatcga aatcagcgcc
     3601 aaccagtacg ctaactgggg gtcgaaaggc gcagcggttg atcgtctcga cgtggtggta
     3661 ctgagcgcgc tggaaattga cacccagttc aacgttaacg tgctgaccgg ctctgacggc
     3721 gtactgcgtg gtgcttccgg tggtcactgc gataccgcga ttgcttctgc gctttccatc
     3781 atcgtcgcgc cgctggtacg cggtcgtatt ccgacgctgg tggataacgt gctgacctgc
     3841 atcaccccgg gctccagtgt cgatattctg gtcacagacc acggcatcgc tgttaacccg
     3901 gcacgtccgg agctggcaga acgtctgcag gaagcgggga tcaaagtggt ttctattgag
     3961 tggctgcgtg aacgtgcgcg tctgctgacc ggtgaaccac agccgattga attcaccgac
     4021 cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga tcgatgttgt gcatcaggtg
     4081 aaggaataag ccatgcacct gcttcctgaa ctcgccagcc accatgcggt gtcaattccc
     4141 gagctgctcg tcagccggga tgaaaggcaa gcacggcaac acgcctggct caagcgccat
     4201 cctgttccac tggtctcctt taccgtggtt gcgcctgggc cgattaaaga cagcgaggtc
     4261 acacgccgaa tttttaatca tggcgtgaca gccttgcgtg ccttagccgc aaaacagggc
     4321 tggcaaattc aggagcaggc tgcactggtt tccgccagcg ggccggaggg catgttgagc
     4381 attgccgccc cggctcgcga cctcaagctc gccaccattg agcttgaaca tagtcatcct
     4441 ctcgggcggt tatgggatat cgatgtcctg acacccgaag gcgaaattct ctcccgccgc
     4501 gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc aaagcgcagc tgtttgcgcg
     4561 cgtggaaaaa cccatcaact gaccgattta ctcaaccgca tggaggcgct gctgaacgat
     4621 gtcgatgcct gcaacgtcaa ctaaaaacac aaagcttgcg acgtcattaa tcgatgagta
     4681 cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat ctgtcaccga aaccaggcct
     4741 cgtggatcgc attaactgcg gtgcgcataa agatatggcg ctggaagatt tccaccgcag
     4801 cgcgctggcg attcagggct ggttaccccg tttcattgaa tttggtgcct gtagtgctga
     4861 aatggcacca gaagcggtac tccacggatt acgcccaata ggtatggctt gcgaaggtga
     4921 tatgttccgc gccactgcgg gcgtaaacac gcataaaggc agcatttttt ctttagggct
     4981 actgtgtgcg gcaattggcc gtttgcttca actcaaccag tcggtaacgc caataacaat
     5041 ttgtgcgacg gcggcaagtt tctgccgtgg cctgaccgat cgcgaactgc gtaccaataa
     5101 ttcacaactg acggcaggtc aacggttgta ccagcagctt ggccttaccg gcgcacgcgg
     5161 tgaagccgaa gcgggttatc cactggtgat caaccacgcc ctgccgcatt acctcactct
     5221 gctggatcag gggttagatc ctgaactggc attgctcgat accttgctcc tactgatggc
     5281 taccaacggc gataccaacg ttgcatcgcg cggtggcgag gggggcctgc gctggctaca
     5341 gcgcgaggcg caaacattat tgcaaaaagg gggcatccga acccccgccg atctcgatta
     5401 tctccggcag ttcgacaggg agtgtatcga acgaaatctc agtccaggcg gcagtgccga
     5461 cctgctgatc cttacctggt ttttagcaca gatttaatta tttaagcact tgataaattt
     5521 ggaaatatta attttcggag aacccgtatg tctttagcaa aagataatat atggaaacta
     5581 ttggccccac tggtggtgat gggcgtcatg tttcttatcc ctgtccccga cggtatgcca
     5641 ccgcaggcat ggcattactt cgctgtgttt gtggcaatga ttgtcggcat gatccttgag
     5701 ccaattccgg caacagcgat cagttttatt gcggttacta tttgcgttat tggcagtaat
     5761 taccttctct ttgatgccaa agaattagct gacccagcgt ttaatgcgca aaaacaggcg
     5821 ctgaaatggg gtctggctgg tttttccagc accacggtat ggctggtatt tggcgcattt
     5881 atttttgcat tagggtatga agtttccggg ttaggtcgtc gtattgccct tttcctggtg
     5941 aaattcatgg gcaaacgcac gctgacgtta ggttatgcga ttgtcattat cgacattctg
     6001 ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg gtacggtttt tccggtcatt
     6061 aaaaacctgc cgccgttgtt taaatcattc ccgaacgatc cgtccgcgcg tcgtattggc
     6121 ggctatttga tgtggatgat ggtcattagt accagtctga gttcgtccat gtttgtcacc
     6181 ggtgcggcac caaacgtgct gggtctggag ttcgtcagca aaattgccgg tatccagatt
     6241 agctggttgc agtggttcct ctgcttcctg ccggttgggg ttatcttgct tatcattgcg
     6301 ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac acagtgaaga agtggcaacc
     6361 tgggcgggtg atgaactaaa aaccatgggt gcgctgacac gcagagagtg gacgctgatt
     6421 ggccttgtat tgctcagctt aggtttgtgg gtatttggca gtgaagtcat taatgctact
     6481 gcggtaggtc tgctggcagt ttcgctgatg ctggcgctgc acgttgtgcc gtggaaagac
     6541 attactcgct ataacagcgc atggaacacg ctggtcaacc tggcaacgct ggttgttatg
     6601 gcgaatggcc tgacccgttc tggctttatc gactggttcg ccaacacgat gagtacgcac
     6661 ctggaaggct tctcaccaaa cgcaacggtg attgtactgg ttctggtgtt ctactttgcc
     6721 cactacctgt ttgccagcct gtctgcgcac accgcaacca tgctgccggt tattctggcc
     6781 gtcggtaaag ggattccggg cgtaccaatg gaacaactgt gtatcctgct ggtgctgtct
     6841 atcggtatca tgggctgtct gacgccgtat gcaaccggtc ctggggtgat tatttacggc
     6901 tgtggctatg tgaaatcaaa agattactgg cgtcttggcg caatcttcgg ggtgatttac
     6961 atctctatgc tgctgttggt tggctggccg attctcgcca tgtggaacta atacgttcgc
     7021 caaacgctga ataaatcatc acgcccgcca ggtaagccca cctggcgggc ttttttatga
     7081 tttaatagat aatattcagt atatgtatga gttccacacc cattatgaaa gcattctggc
     7141 gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc ttctgccaac gccgtggcgt
     7201 tgcaggcaaa acagtatggc gattttgatc gctatgtcct ggccctctcc tggcaaaccg
     7261 gattttgcca gagtcaacac gatcgaaatc gtaacgaacg agatgaatgt cgcctgcaaa
     7321 ccgaaacgac caacaaagct gattttctga ccgtacatgg tctgtggcca ggattgccta
     7381 aatcggttgc tgcccgtggt gttgatgaac gccgctggat gcgcttcggt tgcgctactc
     7441 gcccaatccc gaatctacca gaagcgcgcg ccagccgaat gtgttcatcg ccggaaaccg
     7501 ggttatcact ggaaacggcc gctaaactaa gtgaagtcat gccaggagct ggcggacgtt
     7561 cctgcctgga acgctacgaa tatgccaaac acggtgcctg ctttggtttt gatccagacg
     7621 catacttcgg cacgatggta cgcctgaatc aagaaattaa agagagcgaa gccggaaaat
     7681 tccttgcgga taactacggt aacacagtga gccgccgtga ctttgatgcc gcctttgcca
     7741 aaagctgggg aaaagagaac gtgaaagcag ttaagctaac gtgccagggt aaccctgcgt
     7801 atttaactga aattcagatc tcgatcaaag ctgacgccat caacgctccg ctttctgcca
     7861 actcattctt gccacaacca cacccaggca actgtggcaa aacctttgtg attgataaag
     7921 caggttatta atcctctgat ttacaaaaca cgctaattcg tcttccataa accatgacga
     7981 ttaagattta tacataatgc gctacattga ggaattccct gaatgtgacg caaatcactt
     8041 caaagagtga gcaacagtca gtatcatcat gtgagttaaa ccctcgccgc ctgacggtga
     8101 gggttttctt ttgggtatgt ttatctgcga cactcgcagt accgacgaca tggagtaaaa
     8161 atgtccagac caactatcat cattaacgac ctggatgccg aacgcatcga tattctgctg
     8221 gagcaacccg cctatgctgg tttgccaatc gccgacgcgt taaacgcaga gttggatcgc
     8281 gcccaaatgt gttcgccaga agagatacca cacgacgtgg tgacaatgaa cagccgggtt
     8341 aaattccgca atcttagcga tggcgaagtg cgtgtgcgca cgctggtgta tccggcaaaa
     8401 atgaccgaca gcaatactca gctttccgtt atggctccgg taggtgccgc actgctgggg
     8461 ctgcgcgttg gcgattccat tcactgggaa cttccgggcg gcgttgcaac ccaccttgaa
     8521 gtgctggaac tcgaatacca gccagaagct gctggcgact acctgcttta atcccatctg
     8581 cacaagcgat aactcgcttt gcccagagga tgcactcgca tcctctttcc gctcaaatat
     8641 caaccagatt tatcattttg cgcttaagcg cagcattcta atctgaactg gttattcatc
     8701 tgcggaacct catatgagtg aagctctcgg tatcactgca ttttatatat tcatagccgc
     8761 aattattgtt gctgcagtgc tataccttga acaacgctgg tagtgcctgg ttttatttat
     8821 tttccagctc aatagcaaat tcagatcaaa tcagcctcgc aaattataga ttcataacca
     8881 ataatgtgct gttatgtata catgcaaaaa caaggagaga atgcttatgt acaaaacaat
     8941 catcatgcca gtggatgttt tcgaaatgga attgagcgac aaagcggttc gccacgctga
     9001 attcctcgcc caggatgacg gagttattca tctacttcac gtactaccgg ggtcagccag
     9061 cctgagcctg caccgttttg ccgctgatgt acgtcgtttt gaagagcatc tgcaacatga
     9121 agcagaagaa cgtttgcaaa cgatggtcag ccacttcacc atcgatcctt cctgcattaa
     9181 acaacatgtc cgttttggta gcgtgcggga tgaagtcaat gagttggcag aagaactggg
     9241 ggctgatgtt gtcgttatcg gttcgcgcaa cccatcgatt tcgacccatc tgttaggctc
     9301 taacgcctca agcgttatcc gccacgccaa tctgccggtg ctggtcgttc gctaatagat
     9361 aaaagtattt atgtcgccct gcccggagcc atccctgcag ggctttttaa tgcaataaaa
     9421 aagccgccag gtttgacccc ggcggctaag caattgcagg tgaatcttac ttcttcttat
     9481 gcagttttgg tgcgaatcag gtagtcaaaa gcactcagag aggctttggc accttcgcca
     9541 gtggcgatga tgatctgctt gtacggaacc gtcgtacagt cacccgctgc gaacacgcct
     9601 ttgacgttgg tttcgcattt cgcatcaatg ataatctcgc ccatgcgatt acgttcgact
     9661 gcgccttcca gccagttggt gttcggcagc agaccaatct ggacgaaaat accggccagt
     9721 tcgatgttgt gaatatcgcc gctgacacga tcgcgatatt ccagacctac gactttgcta
     9781 ccgtcgcctt tcacttccgt ggtttgcgca ttcagaataa tgtcgacgtt tttcaggctg
     9841 cgtagtttgt cctgcagaac ctggtcggct ttcatttctg gcgcaaattc cagcagcgtt
     9901 acgtgctcaa cgatacccgc caggtcaatt gccgcttcca cgccggagtt accgccgccg
     9961 ataaccgcta cgcgtttgcc tttaaacagc gggccgtcgc agtgcgggca gtaggtcacg
    10021 cctttggtgc gatactgatc ttcgccagga acgttcatgt tgcgccattt tgcaccagtc
    10081 gccacgataa tgctgcgtgc tttcagtacc gcgccagaag ctgtttcaat ctgatgcagg
    10141 ccgccttcaa ccgctgccgg gatcagtttg ctcgcgctct ggctgtcgat cacatcaacg
    10201 tcgtattcat caacatgaac tttcagcgca cctgccagtt tctggccttc ggtcttcggt
    10261 acagaaatgt agttttcgat atcaacggta tcgaggatct gaccaccaaa acgttcgccc
    10321 atcagaccgg tacggatgcc tttacgtgcg gagtaaattg ctgccgctgc acccgccggg
    10381 ccggaaccaa cgattaatac gtcataagca tcacgcttgt tcagctcttc tgccgcacgt
    10441 ttttccgcac cagtatcaat tttggcaacg atttcagtca acgtcatgcg gccctgacca
    10501 aactctttcc cgtttacgaa cactgccgga acgcccatca cgttgcgatc ggtgatttcg
    10561 ttctggaagg tgccgccgtc aattgcagtg tgcttgatgc gcgggttcag tacgctcatc
    10621 aggttcagcg cctgcaccac gtccgggcag ttgtggcaag agagcgagta ataggtttcg
    10681 aattcaaaat caccgtcaat atggcgaatc tgctccagca gagactgcgc ttctttcgac
    10741 ggatgaccac cggtccacag caacgctagt accagcgagg tgaactcgtg gcccagcggg
    10801 gagcctgcaa aacgtggccc ctggttagaa cctgggttgg tgatcaggaa agacggctta
    10861 cgcaccggca agctgttatc ttctttaaag gtgactttgt ctgacagttc tgcgatttca
    10921 gccaacagtt ccttgatttc tgccgattta gcgctgtcat ccagcgtggc aattaactca
    10981 acaggcttgg tcaatttctc aaggtaagcc ttgagttgag ttttcatatt tgtgtcgagc
    11041 atgtttatct cctgggcttt aaacatcatc atgcaagcgg cctctgcggc tacctggacg
    11101 cagcttgcat catggtgcgg ggaagaaaat cgggtgccgc agcacccgaa gaattaggtg
    11161 aatttccgaa gtatctcgcg ccggagttga tagcgcgaaa gacgaaggaa atttagattt
    11221 taccaaccag gtccagagac ggagccagag ttgcttcacc ttctttccat ttagccgggc
    11281 aaacttcacc tgggtgagaa gctacgtact gtgctgcttt gattttacgc agcaggtcag
    11341 acgcgtcacg gccaatgcct tcagcggtaa cttcgattgc ctggatgata ccctgcgggt
    11401 caacaacgaa ggtcgcacgg tcagccagac cttcatcttc acgcatgttg tcgaagttac
    11461 gggtcagggc gccagtcggg tcgccgatca tcgcatattt gattttagcg atggtttcag
    11521 agctgctgtg ccatgctttg tgggtgaagt gagtatcggt agatactgcg tatacgtcta
    11581 cgcccagttt ctgcaattct tcgtaatggt cagcaacgtc acccagttcg gtcgggcata
    11641 cgaaagtaaa gtcagccggg tagaagaaga agacgctcca gcggccttcg gtatcttttt
    11701 cggtgatttc gatgaattcg ccgtttttga atgcctggtt tttaaaaggt ttaattttgg
    11761 tgttaatcaa ggacatctat acttcctccg tgttttcgat gagatgtaag gtaaccaatt
    11821 tttgccgatt cggctaatgc gtttccatta tcaaatcgat aagttttacc ttacaacctt
    11881 cgtaagacaa ctttgtgaac tttgcaccgc cagcggcgat aaaaagtaaa atggccccct
    11941 ttccaggtga ccgtgtaatc tgaactaccc tttggtatct tcagcgcctg cctgcaggct
    12001 gaggtgttgc ccttacaaat gcaacaacga catggattac aacaccctca taaacaaagg
    12061 gcaatcacct gatctaagct cttacctatg acagtgatag gttatgcctt ttactcgact
    12121 tttgcactga ctgaaaagga caaattaatg ttaaaaaaga tacttttact ggctctgctt
    12181 cctgcaatcg ccttcgcaga ggaacttcct gccccagtaa aagcgattga aaaacagggt
    12241 attacaatca tcaaaacgtt cgatgcccct ggaggaatga aaggttatct cggtaagtat
    12301 caggatatgg gcgtcaccat ctacctgact ccagatggta agcacgctat ctctggttac
    12361 atgtacaacg aaaaaggtga aaacctgagt aacacgctta tcgaaaaaga aatttacgca
    12421 cccgccggac gtgaaatgtg gcaacggatg gaacaatccc actggctcct cgacggtaaa
    12481 aaagatgcgc cggtcattgt ctacgtcttc gccgatccgt tctgcccata ttgtaaacaa
    12541 ttctggcagc aggctcgtcc gtgggtagat tctggcaaag tgcaattgag aacattgttg
    12601 gttggggtta tcaagccaga aagcccggcg acagcagcgg caattcttgc ctccaaagat
    12661 cccgcaaaaa cctggcaaca atatgaagcc tctggtggca agcttaagct aagcgtgcct
    12721 gcaaacgtaa gcacagagca aatgaaagtg ttaagcgaca acgagaaact catggacgat
    12781 ctgggggcaa atgtcacacc agctatctat tacatgagta aggaaaatac cctgcaacaa
    12841 gccgtcggat tacccgatca gaaaacactt aatatcatta tggggaataa atagaatata
    12901 attcatcgat ggttgaaatc tctctctgct tttcaaccat cctaaaaacg catgaattgc
    12961 tcgtaaccca cctgacagct atgctatttt agaaaatctt aatccaaag
//
LOCUS       DAZGJT010000117        12565 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZGJT010000117 DAZGJT010000000
VERSION     DAZGJT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.117"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            346..1317
                     /locus_tag="ACHKFK_003976"
     CDS             346..1317
                     /locus_tag="ACHKFK_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate TRAP transporter
                     substrate-binding protein"
                     /protein_id="HFO9054207.1"
                     /translation="MSKIIAMLITVSTLFLPSIIQAKPISIKVAYENNPGEPLDVVMR
                     YWADLLNKKSNGEITLALYPSSQLGSKQDVTEQAMMGMNVITLSDVAFLADYEPDLGI
                     LFGPYLTDDPQKLFKIYESDWFKQKNESLKKKGIHVVMNNYLYGTRQIISKKPIRTVD
                     DLAGLKIRVPNNVMQIKAIQAMGATPTPMPLGEVYPALTQGVIDGVENPISVLQGQKL
                     YEQARYLTMVNYLTNTSVWIGGEAFFSTLSPEQLEMIHQTGYEAGVYSQKLTLERDAE
                     MLKTMQAAGVEVIYPDTGPFQKKAREVYSQFPEWTPGLYETIQQQLQ"
     gene            1381..1854
                     /locus_tag="ACHKFK_003977"
     CDS             1381..1854
                     /locus_tag="ACHKFK_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO9054208.1"
                     /translation="MRLFGKVAEFSAAFALFVLVVVTIGAVFMRYFIGQPLQWTEEMS
                     GMLMIWVVMLGGVVAERDRAHLTIPFLMEMLPGKLRRVIAVLVALLSIALLLYMAWLG
                     YRLAEMAQFKVTQILKVSWFWIDLAVPVGALATAIYTLYWLINDTKQSDSGDKMQ"
     gene            1851..3122
                     /locus_tag="ACHKFK_003978"
     CDS             1851..3122
                     /locus_tag="ACHKFK_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000118634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO9054209.1"
                     /translation="MSWLLIIPLMIVCLFLNIRVYLAMFIGIIAYFLFFSSVPIEIAV
                     QRLIAPTQSPSLLAIPFFILLGTLMSYTGIAERILHIANVLVGKMRGGLGVANILVST
                     MMGGVSASNLADAAMLSRMMVPEMERKGYNRAFAAAITAGGSLVTPIIPPGIALIIYG
                     LVADVSIGKMFIAGLVPGLLCAVVLMFTVYLVARKTNAKPSRESWPTGKEVVFSLGQA
                     WPALFLIFAVVGGIRANIFTPTEAGAVAVLIVLAIGFFIYREMRISHVVKALGETARA
                     TASVMLVIMASAALGWIFSMEHAGVAVANFVTSLTENKYMFLLIINILLLTLGMFLEG
                     NAILLILVPLLKPVVEHFGIDPVHFGIIMIFNLSIGAFTPPVGTVMLLVCNITQVSVG
                     NFFKQSLPLLAALLLMLMLVTYIPAISLFLV"
     gene            complement(3165..4847)
                     /gene="tsr"
                     /locus_tag="ACHKFK_003979"
     CDS             complement(3165..4847)
                     /gene="tsr"
                     /locus_tag="ACHKFK_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO9054210.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVILAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQQQQQQQRETSAVVKTVTPATPRKMAMADSEENWETF"
     gene            5224..7374
                     /gene="btsT"
                     /locus_tag="ACHKFK_003980"
     CDS             5224..7374
                     /gene="btsT"
                     /locus_tag="ACHKFK_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO9054211.1"
                     /translation="MDTKKIFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVIPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            7492..7695
                     /locus_tag="ACHKFK_003981"
     CDS             7492..7695
                     /locus_tag="ACHKFK_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO9054212.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            7706..8662
                     /gene="yjiA"
                     /locus_tag="ACHKFK_003982"
     CDS             7706..8662
                     /gene="yjiA"
                     /locus_tag="ACHKFK_003982"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO9054213.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEVLCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(8696..8998)
                     /locus_tag="ACHKFK_003983"
     CDS             complement(8696..8998)
                     /locus_tag="ACHKFK_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFO9054214.1"
                     /translation="MSMVKHKRGTSSTLDVQHEAELKALANKFDEDIDYSDIPSSSDE
                     QWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKGYQTRLNAILREAMLRDQNKK"
     gene            complement(8985..9272)
                     /locus_tag="ACHKFK_003984"
     CDS             complement(8985..9272)
                     /locus_tag="ACHKFK_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFO9054215.1"
                     /translation="MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQDRIEN
                     GEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIISARKADRKERNRYEHG"
     gene            9469..10635
                     /locus_tag="ACHKFK_003985"
     CDS             9469..10635
                     /locus_tag="ACHKFK_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO9054216.1"
                     /translation="MKNYKVITPLFPTYAQVKAMMKAVSGYSLKAVRNMITAIHEQTG
                     TPQKPVDWSEPDLWISERLTGEDADIARRIWDTDNHILNPRHSYGCYLFLNYPQFELM
                     ESTPDDIWQPTSRGQKFLQDDEKTLRSLDDQEGILQLLELLAGREMSRRADLLPEWQA
                     FLHQHSKFASASSVKSTLYSRLYNLIDRDMVNREGMSYRITDTGRAWLGKALPARKSD
                     PRKELLEAVKRYNEQQKELLREQISTMNPYKFEQLVGQLLEAMGYEQVEVTKASGDKG
                     VDVIGQVQVGITTITEVVQVKRMQNTITRPYIDQLRGALPYHKAIRGTLITTGKFAAK
                     CAEAALFPGAAPITLIDGDRLLELLIENNVGIRRSNAVELLDVDLQLFDELEMD"
     gene            10699..12318
                     /locus_tag="ACHKFK_003986"
     CDS             10699..12318
                     /locus_tag="ACHKFK_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFO9054217.1"
                     /translation="MAKAPTKKAKAKKGFEETLWDTANQLRGSVESSEYKHVVLSLVF
                     LKFISDKFEARRKKMIADGQADFLEMEVFYQQDNIFYLPEEARWSFIKQNTKQDDIAV
                     RIDTALSTIEKRNPTLKGALPDNYFSRQNLETKKLASLIDTIDNIETLAHETDVETLS
                     KEDLVGRVYEYFLGKFAATEGKGGGEFYTPKCVVTLLTEMLEPFQGKIYDPCCGSAGM
                     FVQSVKFVESHQGKSRDIALYGQELTATTYKLAKMNLAIRGLSANLGERPADTFFSDQ
                     HPDLKADYILANPPFNLKDWRNEAELTKDPRFAGYRMPPTGNANYGWILHMLSKLSAN
                     GTAGFVLANGSMSSNTSGEGEIRAQMIENDLIDCMIALPGQLFYTTQIPVCLWFMTKS
                     KAADPAKGYRDRQGETLFIDARNLGTMISRTTKELTTEDIATIADTYHAWRSTPEELA
                     ARIARGDSKLEKYEDQAGFCKVATLQDIKDNDYVLTPGRYVGAAEQEEDGVAFETKMR
                     ELSKTLFEQMKQAEELDRAIRKNLEALGYGL"
     gene            12308..>12565
                     /locus_tag="ACHKFK_003987"
     CDS             12308..>12565
                     /locus_tag="ACHKFK_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9054218.1"
                     /translation="MGFDFQLLENAGIQLIDGDRGKNYPKHNDFMENGYCLFLSAKNV
                     TKSGFQFQETLFINETKDRELRAGKLKYGDIVLTTRGTVGNV"
BASE COUNT         3083 a         3230 c         3125 g         3127 t
ORIGIN      
        1 cttagttttt atctgtattg acaattaaaa atgaccgcag ccaaattaat gattttattt
       61 ttaataaatc atcaaccgaa taagagatct tatcagcggt aactaaactc atcagagggt
      121 gttccttgta acgtcatgca ttacataact ttaagatata caatcaaaaa atgcattaaa
      181 aaccgattaa aagcagcaaa gtattacccg gtgcacgaat ttatgataca tctgcagcca
      241 ttctgctatt cgctgactac ttttcagtaa tgactgcttt ttcataaggt ttcactcccg
      301 acaacaatgt tggagtttta tttccttagc atggagtaag taaagatgag caaaataata
      361 gcgatgctaa ttactgtcag tacccttttt ttaccgtcaa taatccaggc aaaacctatt
      421 tcaataaaag ttgcctatga aaataaccct ggggaacccc tggatgtggt catgcgttac
      481 tgggcagacc tattaaataa aaaaagtaat ggcgaaataa cacttgcgct ctatcccagc
      541 tcccaattgg gatcaaaaca ggatgtcacc gagcaagcca tgatgggcat gaatgtcata
      601 accttgagtg atgtggcttt tcttgccgat tatgaacccg atttggggat tttgtttgga
      661 ccgtatctga ctgacgatcc gcaaaaactg ttcaaaatat atgaaagcga ctggtttaaa
      721 caaaaaaatg aaagtctgaa gaaaaaggga attcatgtgg taatgaataa ttatctctat
      781 ggcactcgac agattatttc gaaaaaaccc attcgcaccg ttgacgatct cgcaggttta
      841 aaaattcgcg ttcctaataa cgtcatgcaa ataaaagcca ttcaggcgat gggggcaaca
      901 ccaactccca tgccattagg cgaagtttat cccgcattaa cccagggcgt tatcgacggt
      961 gtagaaaacc caatttcagt gctgcaaggg caaaaactgt atgagcaggc aagatatctg
     1021 acaatggtta actacctgac aaatacctca gtgtggatag gtggtgaagc cttctttagc
     1081 accctttcac cagaacaact ggagatgatc caccagaccg gctatgaagc gggtgtctat
     1141 agccagaaac tcactcttga gcgtgatgcc gaaatgttga aaaccatgca ggcagccggc
     1201 gtcgaagtga tctatcccga tactggacca ttccagaaga aagcacgcga agtttactct
     1261 caattcccgg aatggacacc aggtctgtac gaaaccattc aacagcaact gcaataagca
     1321 ttaatgctca gcgtcctgcc tcatctctct taggcaggac acgcactaac aggaggtttc
     1381 atgcgtcttt ttggcaaagt ggcagaattt agtgctgcct ttgcgctatt cgttctggtt
     1441 gtggtgacaa ttggagcggt atttatgcgt tatttcatcg gccaaccgct tcaatggaca
     1501 gaagaaatgt cgggaatgct gatgatttgg gtggtgatgt taggtggagt ggtggcggaa
     1561 cgtgacaggg cgcacctgac cattcccttc ctgatggaga tgcttccagg caaactcagg
     1621 cgtgttattg cagtactggt cgcccttctc tccatcgcac tgctgcttta tatggcctgg
     1681 ctgggctacc gactagccga aatggcgcag tttaaagtga cacaaattct gaaggtttcc
     1741 tggttctgga tcgatctggc ggtccctgtc ggcgcactgg caacggctat ttataccctc
     1801 tactggttaa taaacgatac aaaacaatcc gatagcggag acaaaatgca atgagttggt
     1861 tattgattat tccgctaatg atcgtctgtt tgtttctcaa cattcgggtc tatctggcga
     1921 tgtttattgg catcatcgct tactttttat tcttctccag cgtaccgatt gaaattgccg
     1981 tgcaaagact aatcgcgcca acgcaaagcc catcattgct ggcaattccg ttttttatct
     2041 tgctaggcac actaatgagc tacaccggca ttgccgaacg catcctgcac attgccaacg
     2101 ttctggtggg gaaaatgcgc ggcggactgg gcgtggcaaa cattctcgtc agcaccatga
     2161 tgggcggcgt cagcgcctct aaccttgctg acgcggcgat gctgtcgcgc atgatggtgc
     2221 cggagatgga gcgtaaaggc tacaatcgcg cgtttgccgc cgccattacc gctggtggct
     2281 cgttagtcac cccgatcata ccgcctggaa tagccctgat tatttacggt ctggttgccg
     2341 acgtctctat cggaaaaatg tttatcgccg gactggtacc tggtttgcta tgcgcagtcg
     2401 tactgatgtt taccgtctac cttgtcgccc gcaaaactaa cgctaaaccc tcgcgtgaaa
     2461 gctggcctac gggcaaagag gtggtgttct cgctgggaca ggcctggcct gcgctgttcc
     2521 tcatttttgc tgtggtgggt ggtattcgcg ctaacatttt caccccaaca gaagcaggtg
     2581 cagtagcggt gctaatcgtg ctggcgattg gcttttttat ctatcgtgaa atgcggattt
     2641 ctcatgtcgt caaagcattg ggcgaaacgg cacgcgcaac cgcatcggtg atgctggtaa
     2701 tcatggccag cgccgcattg ggatggattt tctcaatgga acatgctggt gtcgcagtgg
     2761 cgaattttgt gacttcgctg accgaaaaca aatacatgtt cctgttgatt atcaacatat
     2821 tgctgctgac gctggggatg tttctggaag gaaacgccat tttgctgatc ctggtaccac
     2881 tattaaaacc ggtggtcgaa cactttggca tcgatccggt gcattttggc atcatcatga
     2941 tcttcaacct gtcgattggc gcattcacgc caccggtagg taccgtgatg ctgctggtat
     3001 gtaatatcac gcaagtttcg gtgggaaatt tctttaaaca atccctgccc ctactggcgg
     3061 ctctactgtt aatgctaatg ctggtgacat atatcccggc gatttcactg ttcctggttt
     3121 aataataaaa acgccggatg aaatactcat ccggcatcat tacgttaaaa cgtttcccag
     3181 ttctcctcgc tatctgccat ggccatttta cgcggcgtag ctggtgtcac ggtttttacc
     3241 acagccgatg tttcacgctg ctgttgctgc tgttgctgct gttgctgctg ttgctgaatc
     3301 cggaacactg ccactgcttc ggtcaggcga ctggcctgct cttccagcgc ggcggcggcg
     3361 gcggcagatt cttccaccag cgcggcgttt tgttgggtta cccggtccat ctcagcaacc
     3421 gctaagccaa cctgatcgat accacggctc tgctcatcag aagcagaagc aatttcgccc
     3481 ataatgtccg tcacgcgggt cacggcactg acaatctccg ccattgtttc cccagcgctt
     3541 tcgaccagcg tagagccaac atccactttc cccaccgagt cttcaatcag gcttttaatt
     3601 tcacgagcag cctgagcgct acgctgggcc agattacgca cttctcccgc gaccaccgca
     3661 aaaccgcgtc cttgctcacc cgcacgcgca gcctcaaccg ccgcgttcaa agccagaata
     3721 ttggtctgga aggcaatgcc gtcaattacg ctgataatat cggcgatttt ctgcgaactg
     3781 gtggagatat cgcgcatggt ctgcaccacg ttatccacca ctttgccgcc gcgttgcgcc
     3841 gtttcggaag cacttaacgc cagatggctg gcctggcgcg cattttcggc attctgtttc
     3901 accgttgcgg tcagttgctc catgctggct gccgtctctt ccagcgaagc ggcctgttgc
     3961 tcggtgcgcg aagagagatc gttattgccg gtggcgattt cgctggcacc gctatagatg
     4021 gcattggccc cgttgcgcac atcaccgacg gtacgcatca gctctccctg catatggcgc
     4081 aaactctctg ccagttgccc catctcatta gagccatcca cctcaatcgg tttcaccaga
     4141 tcgccgcctg caatatgacg aatgctgtca atcaggcgat tcattggcgc taccagcgag
     4201 gctttaatac cgaaccagac ggcgaggatg accgccagta cgacgatcat cacgcctacc
     4261 agaatccaca tcgcctggct gtaggaggca ttgttatcgc tgacggcgat atcatagagc
     4321 cgatcgtttt gctccatgta agccacatac tgcttctcga aaccgtcctg atatccctgg
     4381 gtcggctgat caaagaactc gttgattttg cctgcaccta acagttgtat cagctccgcc
     4441 agcgcattgt gataaatatc gtaattacgt ttgatctctg ccgctgcggc ggtgctctga
     4501 cgcgggtcac gcggcaacgc ttcgtaatcc gcccagtttt tttccgcctg tttcagcgaa
     4561 atactggcac tctgcatcag ctcagcaacg gttgaaccgc taccaatatt gttctgatcc
     4621 atcatgtagc ggatacccgc gcggttgagg gtgttacgcg tctgcaacaa cgcgacccag
     4681 ctgccattca gcgtggattg ctgctggcga atggtttgta aaacagtgaa attttctttg
     4741 tcgttcttta aggcattaaa gaacagaccg cctgaagtca gttgtaaaag gccaaaaacg
     4801 gccaaaacca gcagtaagct ggtcacaatt ttgatacgtt ttaacatggt ttctctttcc
     4861 tgtggaccga tgcaagattt tcggcctgga aaggaaaaac tttatgaaaa tcccgcggag
     4921 atgacatcga gtaattttat tcaccccctg aatttaaaaa gccattattt acataaatta
     4981 ttcgcaatat aaatatgtga tataaatcac atatttatgg tcacttaaac gacgcctttg
     5041 ccgctcaccg ccattcctga ccgcttacgt ccctaaaata accactcagt tatttacctt
     5101 actttacgcg cgcgtaactc tggcaacatc actgcaggat agcggtcaat ttacctcctc
     5161 aaacgcaacg caaacctaga acggcttcgg ccaactatta atcaatacat gccaggtttt
     5221 actatggata ctaaaaagat attcaagcac ataccctggg tgattctcgg aatcatcggt
     5281 gcattctgcc tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc
     5341 gtggtcgcct ctgtgtcggt gtatctggtg gcgtatcgct actacagtct gtacatcgcc
     5401 cagaaggtga tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt
     5461 ctgaactacg ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc
     5521 ggtgctggtc cgctggtggg tccggttctc gccgcgcaga tgggctacct gcctggcacg
     5581 ctgtggctgc tggcgggggt cgtgctggcc ggtgcggttc aggactttat ggtgctgttt
     5641 atctcctctc gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga gatgggacca
     5701 gtaccgggga ctatcgcgct gtttggctgt ttcttaatca tgatcatcat cctcgccgtt
     5761 ctggcgctga ttgtggttaa agccctggcc gaaagtccgt ggggggtctt caccgtttgc
     5821 tcaaccgtac cgattgcgct gtttatgggt atctacatgc gctttattcg tccggggcgt
     5881 gtgggtgaag tctctgtcat tggtatcgtg ctgctggttg cctctatcta cttcggtggc
     5941 gtgattgctc acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc
     6001 ttcgcgctga ttggctatgc gtttgtttcc gcactgctgc cagtgtggct gatcctcgca
     6061 ccgcgcgact atctggcaac cttcctgaaa atcggcgtta tcgtcggcct ggcgctgggt
     6121 atcgtggtgc tgaacccgga actgaaaatg cctgccatga cccagtacat tgacggtact
     6181 ggcccgctgt ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg
     6241 gtatctggct tccacgcgct gatctcttcc ggtacgacgc cgaaactgct ggctaatgaa
     6301 accgacgcgc gtttcatcgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg
     6361 gcgctggttg ctgcgtccat catcgaaccg ggtctttact tcgcgatgaa caccccgcct
     6421 gctggccttg gcatcaccat gcctaacctg catgaaatgg gtggcgagaa cgcgccgatc
     6481 atcatggcac agctgaaaga cgttaccgca cacgcggcag cgaccgtcag ctcctggggc
     6541 ttcgtgattt cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc
     6601 ctgaaccgtg caggtggtgc gccaacgctg gcggtaggta tcgctcacgt gttccacaaa
     6661 gtgctgccga tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg
     6721 ttcatcctga ccgcgctgga tgcgggtacc cgttctggcc gctttatgct gcaagacctg
     6781 ctgggtaact tcatcccgtt cctgaaaaaa accgactctc tggttgccgg tatcatcggt
     6841 actgcgggct gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg
     6901 ggcggcgtta agagcctgtg gccgctgttc ggtatctcca accagatgct ggcagccgtg
     6961 gcactggtac tgggcaccgt tgtactgatt aagatgaagc gcacccaata catctgggtt
     7021 accgttatcc cggcagtatg gctgcttatc tgcaccacct gggcgctggg cctgaaactg
     7081 ttcagcacca acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag
     7141 attgctaacg gtactgacct gacggcgcag cagattgcca acatgaacca catcgttgtg
     7201 aacaactaca ccaacgcagg cctgagcatt ctgttcctga ttgtggtgta cagcatcatc
     7261 ttctacggtt tcaaaacctg gctgaaggtg cgtaacagcg acaaacgtac tgacaaagaa
     7321 acaccgtacg ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccggtt
     7381 gccttatccg gcttacaaag tgtctatcgt aggccggata aggcgtttac gccgcatccg
     7441 gcaaatccct agcccggacc ttccgggctt tacgcatatc tggaaggcaa aatgtttggt
     7501 aacttaggac aggcaaaaaa atatctcggc caggcggcga agatgttgat tggcattcca
     7561 gactacgaca actacgtcga gcatatgaag accaaccatc ccgacaagcc gtacatgagc
     7621 tatgaagaat tcttccgcga acgccagaac gcgcgctacg gcggcgatgg taaaggcggt
     7681 atgcgctgtt gttaatggag agagcatgaa tccgattgca gttaccctac tcaccggttt
     7741 tttaggcgca ggaaaaacca ccctgctgcg ccatattctt aacgaacaac acggctacaa
     7801 gattgccgtg attgaaaacg aattcggcga agtctccgtt gatgatcaac tgattggcga
     7861 tcgcgccacg cagatcaaaa cgctgaccaa cggctgcatc tgttgttcgc gctccaacga
     7921 gctggaggac gcgctactcg acctgctgga caatctcgac aagggcaata ttcagttcga
     7981 tcgtctggtt attgaatgca ccggcatggc cgatcccggc ccgattattc agaccttttt
     8041 ctcccatgaa gttttatgcc agcgttacct gctggacgga gtgattgcgc tggtggatgc
     8101 ggtacatgcc gatgagcaga tgaaccagtt caccatcgcc cagtcacagg ttggctacgc
     8161 cgaccgtatt ctgctgacta aaaccgacgt cgcaggcgaa gcagaaaaac tgcgtgaacg
     8221 cctggcgcgc atcaacgccc gcgcaccggt ctacaccgtc acccacggcg acatcgacct
     8281 gggcctgctg ttcaacacca acggttttat gctggaagaa aacgtcgtca gcaccaagcc
     8341 acgttttcac tttatcgcgg acaaacaaaa cgatatttcg tcgattgtgg tggaactgga
     8401 ttacccggta gatatcagcg aagtttcccg cgtgatggaa aacctgctgc tggagtcggc
     8461 ggataaacta ctgcgttaca aagggatgct atggattgac ggcgaaccta accgcctgct
     8521 gttccagggc gtccaacgcc tctacagcgc cgactgggac cgcccgtggg gcgatgaaaa
     8581 accgcatagt acgatggtgt ttatcgggat tcagttgccg gaagatgaga ttcgggcagc
     8641 gtttgcgggg ttaaggaagt aatttgggat gatggccgcc atgtggcggc ctgaattatt
     8701 ttttgttctg atcgcgcagc atcgcttcac gtaaaattgc gttcaggcga gtttgatagc
     8761 ctttaccagg tcgcttcaac cattccatca catcagcatc aatacgcacc gaagcctgag
     8821 ttttcaatgg ccgataaaac ttaccgcgtt cggcgttaga ccattgctca tcgctggaag
     8881 atgggatgtc gctgtaatca atatcctcgt cgaatttgtt cgccagcgcc ttcagttcgg
     8941 cctcatgctg cacatcgagt gtagaggagg tccctcgttt atgtttaacc atgctcatag
     9001 cgattcctct ctttacgatc tgcttttcgt gcactgatga ttcgaataat ttcatttccg
     9061 ctttcgaaac ggatggtatg cgcaaccaga ataaccacga tgccgtaaac caggccgatt
     9121 gtctgccagc gatactcacc gttttctatg cgatcttgct gtgacaaatg ttgtggatca
     9181 tcaaacacta acaccgcatc ttcaaagcgg atgccatgtt ttacccgatt acttttggcc
     9241 ttgtttgcat cccattcaaa ctccattggc atagttgcca tccctaattg tatatacaaa
     9301 aatgtatcta caattattct gcaattatag cgttgatgtt ggaaatagca tcacgttttg
     9361 gataatcaac catgaaaatg acaatgagtg agttggactt gattcatcca atagaatttc
     9421 tcgcctgaca tggcacacta aacacctcgc tgtcgttaag ggattaccgt gaaaaactac
     9481 aaagtcatta ccccgctttt tcctacctat gcgcaagtta aggctatgat gaaagccgtc
     9541 tcaggctatt ccctgaaagc ggtacgcaat atgattaccg ccattcacga gcaaaccggt
     9601 acgccgcaaa aacccgttga ctggtcagaa cccgacctgt ggatcagcga gcgcttaacg
     9661 ggtgaagatg ccgacatcgc ccgtcgtatt tgggataccg ataatcacat cttgaacccg
     9721 cgccacagct acggctgcta cttattcctc aactatcccc agtttgagct gatggaaagc
     9781 acaccagatg atatctggca gcccacctcg cgcgggcaaa aattcctgca agatgacgaa
     9841 aaaacgctgc gttcactgga cgaccaggaa ggaattctgc aactgcttga gctgctggct
     9901 ggccgggaaa tgtcgcgccg cgccgattta ctgccggaat ggcaggcctt tttgcatcag
     9961 cattcgaaat tcgcgtctgc aagttcagtg aaaagcacgc tctactcccg tctctataac
    10021 cttatcgacc gtgacatggt taaccgtgaa ggcatgagct atcgcatcac cgataccggg
    10081 cgcgcctggc ttggaaaagc gttgcctgca cgaaaaagcg atccgcgtaa agagctgctg
    10141 gaagccgtga agcgctacaa cgagcagcaa aaagagctgc tgcgcgaaca aatcagcacg
    10201 atgaacccgt acaaatttga acagctggtc ggtcaattac tggaagcaat gggctacgag
    10261 caggtcgaag tgaccaaagc ctctggcgat aaaggcgtgg acgtgattgg tcaggtgcag
    10321 gtcggcatca ccaccataac cgaggtggtg caggtcaaaa gaatgcagaa caccatcacc
    10381 cgcccttata tcgaccagtt gcgcggggca ttgccttacc acaaagccat tcgcggtaca
    10441 ctcatcacca ccgggaaatt tgcggcgaaa tgtgcagaag cggcgctctt tccgggtgcc
    10501 gcgcccatta cgcttatcga cggcgatcgt ttactggaac tgttaatcga aaataacgtc
    10561 ggcattcgcc gcagcaatgc agttgaattg ctggatgtag atttgcagtt gtttgatgag
    10621 ctggagatgg attaaagggc gaaatccgcc gcctcaacca ttacaatagc gacccaattt
    10681 atctgaacag agttcatcat ggccaaagca ccaactaaaa aagccaaagc aaagaaaggc
    10741 tttgaagaaa cattatggga tacagcaaac cagcttcgcg gtagcgttga gtcctccgaa
    10801 tacaagcacg tggtgttgag cctcgtgttc ctgaaattca tcagcgataa gtttgaagca
    10861 cgccgcaaaa aaatgattgc cgatgggcaa gcagacttcc ttgagatgga agtgttctac
    10921 cagcaggaca acattttcta cctgccggaa gaggcgcgct ggtcatttat caaacaaaat
    10981 accaaacagg acgatattgc ggttcgtatt gacaccgccc tctcgaccat tgagaaacgt
    11041 aacccaaccc tgaaaggcgc gctgccagac aactacttca gccgtcagaa tctggaaacc
    11101 aaaaaactgg cgtcactgat tgataccatc gacaacatcg aaacgctggc acacgaaact
    11161 gacgttgaaa cgttatcgaa agaagacctg gtcggacgcg tttacgaata cttcctcggt
    11221 aagtttgccg caaccgaagg caaaggcggt ggtgagttct acacgcccaa atgtgtggtt
    11281 acgctgttaa ctgaaatgct cgaacccttc cagggcaaaa tttatgatcc gtgctgcggc
    11341 tcggcgggga tgtttgtgca gtcggtgaag tttgttgaga gccatcaggg taaaagtcgt
    11401 gatatcgcgc tgtatggtca ggaactgaca gccacgacgt ataaactggc aaaaatgaac
    11461 ctcgccattc gcggtctttc agctaacctc ggtgaacgac cggcagacac cttctttagc
    11521 gaccagcacc cggacctgaa agccgactac attctggcga atccgccctt caacctgaaa
    11581 gactggcgta atgaagccga attaaccaaa gacccacgtt ttgctgggta tcgtatgccg
    11641 ccaaccggta acgccaacta cggctggatt ttgcatatgc tctccaagct gtcggctaac
    11701 ggcacagcag gttttgtgct ggcaaacggt tcgatgagtt ctaacaccag cggtgaaggc
    11761 gagatccgtg cgcagatgat cgaaaatgat ctgatcgact gcatgatcgc cctacctgga
    11821 cagttgttct acaccacgca gatcccggtg tgtttatggt ttatgaccaa atcgaaggct
    11881 gccgacccag ccaaaggtta tcgtgatcgt cagggcgaga cgctgtttat tgatgcgcgt
    11941 aacctcggca ccatgattag ccgcacaact aaagagttaa cgacggaaga tattgccaca
    12001 atcgccgata cctaccatgc ctggcgcagc acgccggaag aactggctgc acggattgcg
    12061 cgtggtgaca gcaagctgga aaaatatgaa gaccaggcag gcttctgcaa agttgcgacc
    12121 ctgcaagata ttaaagataa cgactacgtg ctgacgccgg gccgctatgt gggtgcagcc
    12181 gagcaggaag aggacggcgt ggcatttgag acaaaaatgc gcgaattgtc gaagacgttg
    12241 tttgagcaga tgaagcaggc ggaagaactg gatcgtgcga ttcgcaagaa tctggaggcg
    12301 ctgggttatg ggctttgatt ttcagttgtt agaaaatgca gggatccagc taattgatgg
    12361 tgacagaggc aaaaattatc ctaaacataa tgattttatg gagaatggct attgtctttt
    12421 cttatccgct aaaaatgtca ctaaatctgg ttttcaattt caagaaacat tatttatcaa
    12481 tgaaactaaa gatcgtgaac taagagcagg aaagcttaag tacggtgaca ttgttcttac
    12541 tacccgtggc acagtaggta atgtt
//
LOCUS       DAZGJT010000118        12396 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZGJT010000118 DAZGJT010000000
VERSION     DAZGJT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.118"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..829
                     /locus_tag="ACHKFK_003988"
     CDS             <1..829
                     /locus_tag="ACHKFK_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HFO9054219.1"
                     /translation="DELDEMTCGLQPGDLILLAARPSMGKTALGLTLCLNALRERTDE
                     TVFLFSIEMSREQLILRTLSMLSRVELTRLRSSMMDDEEWARFTATMGEFMQEGTFAG
                     EGNRLIIDDTSSQTPSSLRASARRYVRLYGRPSLIMVDYLQLIRSPDQENRTQEIAEI
                     SRALKSLGKELGCPVLALSQLNRQVEQRADKRPNNGDLRDSGALEQDADLIMFIYRDE
                     VYNPGTLDKGVAEIIVSKQRQGPTGTIRVSFDGRYTLFSPFERGWNFDRSGGNAYVN"
     gene            819..2444
                     /locus_tag="ACHKFK_003989"
     CDS             819..2444
                     /locus_tag="ACHKFK_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO9054220.1"
                     /translation="MSIKRNHSLDLGAAIMQPGRQASKTGNVVALGETPMVLTLDQLR
                     PNPDNPRTTRNPRYDDIKSSIFSRGLDTVPKVTRIPESEPDIYIFSDGGNTRYQILRE
                     LWEETGEQRFYRIHVLFKPWPGRLQCVIGHLAENEVRGELTFIEKAQGIHKARMIYEE
                     QLSKSVSMRELSSLLTEQGLPVDASSISRMENALKYLYPWIPELLESGMGRPQVNSLL
                     SLRQDAERIWGQFAVAAENDVDFDRVFGECCGKFNSPELWSMEMFRDELIGDLLKALP
                     HPVLDYDRWLLELDPRERNRRHHFGEPEPVLMPADSPPEPGAQVASGGATEAAQVVAA
                     TPADSESVMDTAQSGAEKEDRPVADKRERRRKEVQPDMYGGEDSYSGDCGEDATGAST
                     DIPIMPEDNAMQEGGEFRQASAFTGAEQDDIESLQNEAFHLAWELASHTGCVDEIAMD
                     RESELSAGFGAAEANCSPVMAFLVRLTGEEVLRVNPVSLDALLIGGSTEEDLPLLDDE
                     HAGKLLRLLGVLRRLRGLQRMVEDESEEGRDEC"
     gene            2434..3165
                     /locus_tag="ACHKFK_003990"
     CDS             2434..3165
                     /locus_tag="ACHKFK_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HFO9054221.1"
                     /translation="MNADAARQDQLIKRIRKLLALSRNNSNPHEAGQALSRAQALMRK
                     HGITELDTDISVIKTSSSKGAPSEAEKVPVWMGRLMHVVAEAFGCHTYLDWRITTGRY
                     WRRVVIFYGFGERPVVAAYAFDVLCRQMQKATKAYLSTQSRTLKLSTRRARAAMFRDG
                     WVYGVRDVVDAFPLTEDETRVMKTWLKRQQFEGMAAFRNVKDCNGGDRARWQGYSEGK
                     KATLHHGVGSRNLRPESLISGGGEV"
     gene            3162..3740
                     /locus_tag="ACHKFK_003991"
     CDS             3162..3740
                     /locus_tag="ACHKFK_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2857 domain-containing protein"
                     /protein_id="HFO9054222.1"
                     /translation="MSNNLSQATNGLLMQLVMDLKSGYLRRCEALGLSREEMQMLQGL
                     SIEEIHYLSNSEVSVLRLDINHNNLVRMLQQARTEQKRLQRIDRALALGGSIELMAFY
                     FGLSSVDVAARRRISGIDVRPGRGITLSDDENSELWRLWQKAGINDVESVDGLDVMML
                     CAEQMNIPLTAIWHAVRGWHTTGNPDQVRSAS"
     gene            3737..3985
                     /locus_tag="ACHKFK_003992"
     CDS             3737..3985
                     /locus_tag="ACHKFK_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054223.1"
                     /translation="MKMTELVPGVLTSPGVPPASQRKAVELVNRFRRGERNYHELKER
                     GFGYFKIDVGPDWRLLSRNKGLTWALLSHERYNNMRLK"
     gene            3995..4252
                     /locus_tag="ACHKFK_003993"
     CDS             3995..4252
                     /locus_tag="ACHKFK_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054224.1"
                     /translation="MSNAARLQLGFSPFSKTIVLAKMRNFDDGTSLRVGNDPGRDVTN
                     EAAQLVWHLVMAEGGEIMWEMDDGSRMVLKAESQEATSEQD"
     gene            4287..4847
                     /locus_tag="ACHKFK_003994"
     CDS             4287..4847
                     /locus_tag="ACHKFK_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9054225.1"
                     /translation="MHDDWGFDTDLFHELVTPSIVQALLDEREAQSKHVAELEANLSA
                     MTEDHQKATESIKQADSAVKLAHETFSALAAENAKLKKFCKDAAFDADYEAESGMERG
                     GFSDALNEIKITATDAFLAEVRAQGVDAAIDAAKNLVAQEYEYKDFKAAQSDCCMFPG
                     SDLVGKVEMTEWLVDFAAELRKGGNQ"
     gene            4844..5581
                     /locus_tag="ACHKFK_003995"
     CDS             4844..5581
                     /locus_tag="ACHKFK_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO9054226.1"
                     /translation="MNKIDYQALREAAEAIKIAATPQKLLAFRMKVTPQVVLALLDEL
                     ETKEEQRANWFQMAQKLGEDLDAEEKRNAEQREYYEGVIADGSKRIAELEKSEEQLIN
                     ERDHAESALADMYFAVTRNRPEWSNCFSFSDAVDAVVDRIADLEAKQPSPVVPEEMYW
                     QDAPVEGSSKATAYATGWNACRAATLQGPEGLIKAVRFYEQVKRENPPVETGAWKDAV
                     DWVLQEACQAVHWCRQISALQEQENHS"
     gene            5578..5793
                     /locus_tag="ACHKFK_003996"
     CDS             5578..5793
                     /locus_tag="ACHKFK_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054227.1"
                     /translation="MRKISGMLNLEKARMQLRVLLSGPVPAITPMAHAVAIKVVLDAL
                     DASERRVAELEQVCTAAGIRIKGEQVK"
     gene            5790..6017
                     /locus_tag="ACHKFK_003997"
     CDS             5790..6017
                     /locus_tag="ACHKFK_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054228.1"
                     /translation="MKLKMQTISGPVIIDSNEVTQFYPDHESGGELTAVEYLTDGGRC
                     TTRVRHSFYQVAEALASAWHVDDASKRNRRG"
     gene            6281..7567
                     /locus_tag="ACHKFK_003998"
     CDS             6281..7567
                     /locus_tag="ACHKFK_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4528 family pathogenicity island replication
                     protein"
                     /protein_id="HFO9054229.1"
                     /translation="MALPAESLIAHTLRKMKASMAEKASREEGRIRSGLLFTGNVHDA
                     VPRRLLLDTRLSPLDKTAWMMIRLHALSNEGAVFPTYDELQLQLASPGSGKASRETVS
                     RVLTMLRLTGWLSLCKRVRDEKGRIRGNIYALHDEPLGCCDAEKFDPRYLDMVLDSAC
                     KNRNKTIRYTAREVLSDIKNEPGMRHYHSHITLFESRMGAAQTPSQMAARQRVNPAVN
                     GRAAKSGSESAQPPVCASVTELSAQVTELCTAVTEPGEKTEVTQRSTDSEQPGKSVGY
                     HSVRKPNHYVRNITHSMKKNTYVLPDALKSQLSVEDTGMLTKQLQALPETLAEGVLDS
                     LSQMLSKNTLQNPVGWMLAVMKRAREGNFNSPDMSRSGTIQSQPARPSSQSGHGCDER
                     ERRRREVASPEKARNIVAEFRASIQRANAARLAMRG"
     gene            8046..8765
                     /locus_tag="ACHKFK_003999"
     CDS             8046..8765
                     /locus_tag="ACHKFK_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL_4669 family integrating conjugative element
                     protein"
                     /protein_id="HFO9054230.1"
                     /translation="MPEEIVKEPASHRVGAFTSTLNIELHSRYAIMLWEGRRGSKAKN
                     NEGRKVPRIMSMPEAIKMTGGLYRDSLADNPYADETMWKLENAIIDATEKINAEVAQL
                     DDILRNIPSGITIKDVLSRSPVNIGVFSRSPLGYRCVWLLVGYDQLALKTFQAAHLGL
                     ISRNQRDELLSFGAYLIRKIYGILQIYRSVGVTRDDIENKTSKGEKAIARYGMPEPDI
                     LSGKARSSFSPPLNRKSRNLS"
     gene            8788..10790
                     /locus_tag="ACHKFK_004000"
                     /pseudo
     CDS             8788..10790
                     /locus_tag="ACHKFK_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682052.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_function: GO:0098847 - sequence-specific single
                     stranded DNA binding [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            11415..11879
                     /locus_tag="ACHKFK_004001"
     CDS             11415..11879
                     /locus_tag="ACHKFK_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016151303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STY4534 family ICE replication protein"
                     /protein_id="HFO9054231.1"
                     /translation="MSATNTSSAKAEYFNLTVKGLGYLSNVCQVNHQNGSFLCCVINA
                     LSGPTDNPSYTRFDVTVVGKDATTLISRCQKAVDEDRKVLLGFALSNLATDIFTLNKG
                     EHAGEQRVSLKARLIKVDWIKIGQEQVYKAEKAESLPPQNGTTTRQYAENSF"
     gene            11973..12179
                     /locus_tag="ACHKFK_004002"
     CDS             11973..12179
                     /locus_tag="ACHKFK_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054232.1"
                     /translation="MNRQNGILAAAAGYTLPLEILKSTRGYYIGTQCSEGPVSRESEE
                     YFKKHDQAEQALKNGTWTQRYDWQ"
     gene            12189..>12396
                     /gene="ssb"
                     /locus_tag="ACHKFK_004003"
     CDS             12189..>12396
                     /gene="ssb"
                     /locus_tag="ACHKFK_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO9054233.1"
                     /translation="MASRGVNKVILLGHLGQDPEVRYFPNGGSAVANLSLATSDTWKD
                     KQSGEMKEVTEWHRIVIYGKLAEIA"
BASE COUNT         3153 a         2707 c         3646 g         2890 t
ORIGIN      
        1 tgatgaactg gatgagatga cctgtggcct gcagcccggg gatcttatcc ttctggcagc
       61 acgtccgtcg atggggaaaa cggcccttgg actcactctg tgtcttaatg ccctgcgtga
      121 gcggaccgat gaaacggtat tcctgttcag catagaaatg tccagggagc agctgattct
      181 gcgtaccctg tcgatgctgt cccgggtgga gctgactcgt cttcgcagca gcatgatgga
      241 cgatgaggag tgggcgagat ttacggcaac aatgggtgag ttcatgcagg aggggacatt
      301 tgccggtgaa ggtaaccgcc tgatcattga tgacacgtca tctcagactc cgtcatcact
      361 gcgcgccagt gcccgtcgtt atgtgcgtct gtatggcaga ccttccctga taatggtcga
      421 ttatctccag ttgattcgtt caccggacca ggaaaaccgc actcaggaga ttgcggaaat
      481 atcccgagcc ctcaagtcgc tggggaaaga acttggatgc cctgtgctcg cactgtcgca
      541 gcttaaccga caggtggaac agcgggcaga taaacgacca aacaacggtg acctgcgtga
      601 ttccggggcg ctggagcagg atgctgacct cattatgttt atttaccgtg acgaggttta
      661 caaccccggc actctggata agggcgtggc ggaaatcatt gtgagcaaac agcgacaggg
      721 accgaccggt acgatacggg tgagcttcga tggtcgttac accctgtttt caccgtttga
      781 acgtggctgg aattttgacc gttccggagg caatgcgtat gtcaattaag cgaaatcatt
      841 cccttgacct gggggccgcc atcatgcaac cggggcgcca ggccagtaag accggtaacg
      901 tggttgccct gggggaaacg cccatggttc tgacgctgga tcagttgcgc cctaatcctg
      961 ataacccgag aaccacgcgc aatccccggt acgacgatat taaaagttca attttctccc
     1021 gcggactgga tacggtgcca aaggtcaccc ggataccgga gagtgagccg gatatctaca
     1081 tcttcagcga tggtggtaac actcgttatc agatcctgag agaactgtgg gaggagaccg
     1141 gcgagcagcg tttttaccgt attcatgtgc ttttcaaacc gtggccgggg cggcttcagt
     1201 gtgtcattgg tcatctggct gagaatgaag tcaggggcga actgacgttt attgaaaagg
     1261 ctcagggtat ccataaagca cggatgattt atgaagagca attaagcaaa agtgtgtcga
     1321 tgcgtgaact gtcatctctt ttgactgagc agggattgcc tgtggacgca tccagtatca
     1381 gccggatgga gaacgcactg aaatatctgt atccgtggat cccagaacta ctggaatcag
     1441 gaatggggag accgcaggtc aattctttgt tatctctccg tcaggatgcg gaacgcatct
     1501 gggggcagtt tgcagtcgcg gcggaaaacg atgtggattt tgatcgcgtg tttggtgaat
     1561 gttgcggcaa attcaactcg ccggaactct ggtccatgga aatgttcagg gatgagctga
     1621 tcggtgacct gctgaaggct ctgccacatc cggtactgga ttacgatcgc tggttgctgg
     1681 agctggatcc ccgggagcgt aatcgacgtc accattttgg tgaacccgaa ccggtactca
     1741 tgccggcgga cagccccccg gaaccggggg cgcaggtggc gtcaggcggg gcgacggaag
     1801 ctgctcaggt cgttgccgcc actcccgcag acagtgagag cgtcatggat actgcgcaat
     1861 caggggctga gaaagaggac cgcccggtcg cagataaacg cgaacgacgc agaaaggaag
     1921 ttcaaccgga tatgtatggt ggcgaagact catacagcgg tgattgcgga gaggatgcga
     1981 ccggtgcgtc aactgatata ccgataatgc cggaagataa cgccatgcag gaggggggag
     2041 aatttcgcca ggcatctgca ttcactggtg ctgaacagga tgatatcgaa tctctgcaga
     2101 atgaggcgtt tcacctcgca tgggagctgg cgtcacatac aggctgtgtc gatgagatag
     2161 cgatggaccg ggaaagtgag ctgtctgcag gatttggtgc ggcagaggca aattgctcac
     2221 cggtcatggc atttcttgtc agactgaccg gtgaggaggt tctgcgggtc aatccggttt
     2281 cccttgatgc actgctgata ggcggcagta ctgaagaaga cctgccgttg cttgatgatg
     2341 aacatgcagg aaagttgctt cgcctgctgg gtgtgttgcg tcgtctgcgg gggctgcagc
     2401 gtatggtgga agacgagagt gaggaagggc gcgatgaatg ctgatgccgc acgtcaggat
     2461 caactgatta aacggataag aaaactgctg gcgctgagcc ggaataacag taacccccat
     2521 gaggcaggcc aggcactttc ccgtgctcag gcgctgatgc gcaagcacgg tatcactgaa
     2581 ctggatactg atatttcggt gataaagacc tcgtcatcaa agggggcccc gtcagaagcg
     2641 gaaaaagttc cggtatggat ggggcgcctt atgcatgttg ttgcagaggc attcggatgc
     2701 catacatacc tggactggcg tatcacaacc ggaaggtact ggcggcgggt tgtcatattt
     2761 tatggctttg gggagcgccc ggttgtggcg gcgtatgcct ttgatgttct ctgccggcag
     2821 atgcaaaagg caacaaaggc gtatctttca acccagagcc ggacattaaa attgtcaacc
     2881 cgaagggcaa gagcagccat gtttcgtgac ggctgggttt atggggtgcg tgatgtggtg
     2941 gatgcattcc cgctcacgga agatgagacg cgtgtaatga agacgtggct gaagagacag
     3001 caatttgagg gaatggctgc cttccggaat gttaaggact gtaatggtgg tgatcgtgca
     3061 cgctggcagg gatacagcga gggtaaaaag gcaacactgc atcatggtgt tggttcccgg
     3121 aatctgagac ctgaaagcct gatatcgggc ggaggtgagg tatgagtaac aacttatcac
     3181 aggccaccaa cgggctgttg atgcaactgg tgatggatct caagagtggc tatctgcggc
     3241 gatgtgaagc gctggggctt tcccgggaag aaatgcagat gctgcagggg ctgagcattg
     3301 aagaaattca ctatctgtca aattcagaag tgtcggtgct gcggctggat attaaccaca
     3361 acaatcttgt gcgcatgttg cagcaggcgc ggactgaaca gaaacgtctg cagcgaatcg
     3421 atcgcgctct ggcgctgggt gggtctatcg aactcatggc gttttatttt ggtctttcca
     3481 gcgtggatgt ggctgcgcgg cgtcgtatct ccggtattga tgttcgtccc ggaaggggga
     3541 tcaccctgag tgacgatgag aattctgaac tctggcggtt gtggcagaaa gccgggatca
     3601 atgatgtgga gagtgtggat gggctggatg tcatgatgct ttgtgctgaa cagatgaaca
     3661 ttccactgac ggccatctgg catgccgtca gagggtggca tacaaccgga aatccggatc
     3721 aggtaaggag tgcgtcatga agatgacaga actggttccc ggagtgctga caagtccggg
     3781 cgtccctccc gccagtcagc gtaaggcggt tgaactggtc aatcgcttcc gtcgtggaga
     3841 gcggaattat catgaactga aggaaagggg atttggttat ttcaagattg atgttggtcc
     3901 tgactggcgg ttactgagcc ggaataaagg gctgacctgg gccctgttga gtcatgaacg
     3961 atacaacaac atgagactga agtgaggtgg aaagatgagt aatgcagcaa gactccagct
     4021 gggtttttct ccgttttcaa aaactatcgt gctggcaaaa atgcgtaatt ttgacgacgg
     4081 aaccagtctt cgtgtcggta atgatcccgg tcgcgatgta accaatgagg cggctcagct
     4141 tgtctggcat ctggtcatgg cagaaggtgg tgaaattatg tgggaaatgg acgatggctc
     4201 ccgcatggtg ctgaaggctg aaagtcagga ggcaaccagt gagcaagatt aactaccagg
     4261 cactgcgtga ggcggcagag caggcaatgc atgacgactg gggatttgac acggatcttt
     4321 tccatgagct ggtaacacca tcgattgtgc aggcgctgct ggatgaacgg gaagcccaaa
     4381 gcaaacacgt agctgaactg gaggctaatt tatcggcgat gaccgaagac caccagaaag
     4441 cgactgagtc aattaagcag gctgattcgg ctgttaagtt ggcacacgag acgttttcgg
     4501 cgctggcggc ggagaatgca aagctgaaga agttctgcaa agacgctgca ttcgatgccg
     4561 attacgaagc agagtcaggt atggagcgtg gtggattcag tgatgcgctt aacgaaatca
     4621 aaatcacagc caccgacgct ttcctggctg aagtacgggc gcagggggtg gatgctgcta
     4681 tagacgctgc aaaaaatctg gtggctcagg aatatgagta taaggatttc aaagcggcgc
     4741 agagtgattg ctgtatgttt cctggttcag acctggtagg gaaggttgaa atgaccgagt
     4801 ggttagttga ctttgccgca gagcttcgca aaggaggcaa ccagtgaaca agattgacta
     4861 tcaggcactg cgtgaggcgg cagaagcaat aaaaatagca gccacaccac aaaaattgct
     4921 ggcatttcgt atgaaagtca cacctcaggt tgtgctggcg ctgctggatg agctggaaac
     4981 caaagaagaa cagcgagcta actggttcca gatggcgcag aagttaggcg aggatttgga
     5041 tgcagaagaa aagcggaacg ccgaacagcg tgagtattac gagggagtaa tcgcggatgg
     5101 aagtaagcgc attgcagaac tggagaaaag tgaagagcaa ctcattaatg agcgtgacca
     5161 tgctgagtct gctttagctg atatgtactt tgcagtaacc aggaacaggc ctgagtggag
     5221 taactgtttc agtttttcag atgccgtcga tgccgtggtt gacagaattg ctgatttaga
     5281 agctaaacag ccatcgccag tggtgccgga agaaatgtat tggcaggatg caccagtgga
     5341 aggcagcagt aaagcgactg catacgcgac aggctggaat gcttgtcgtg ccgccacgct
     5401 tcagggacca gaaggattga ttaaagcagt gcgcttctat gaacaggtta agcgtgaaaa
     5461 tccgccagtc gaaaccggag catggaaaga cgctgttgac tgggtattac aggaagcatg
     5521 tcaggctgtt cattggtgcc gacagatatc agcccttcag gaacaggaga atcattcatg
     5581 agaaaaatca gtggaatgtt gaatctggag aaggccagga tgcagttgcg tgttttgtta
     5641 tctggacctg ttcctgcaat cactccgatg gcgcatgccg tggcaataaa agttgtgctt
     5701 gatgctctgg atgcgagtga gcggagggtg gctgaactgg aacaggtctg tactgccgcg
     5761 ggtattcgta tcaaagggga acaagtgaaa tgaaactgaa aatgcagaca atttcgggtc
     5821 ctgtgattat cgacagtaat gaggtgacac agttttaccc ggaccatgaa agtggagggg
     5881 aactcaccgc tgttgaatac cttactgacg ggggaagatg caccacccgg gtacgccatt
     5941 ccttttatca ggttgctgag gcacttgcca gtgcatggca tgtggatgat gcatcaaaaa
     6001 ggaatcgccg tggataacct gaggtgccgg gggtatgtca gttctgaagc tgctgaaccg
     6061 tgacataaat tttttatgtt gcggtgaagc agccaacaaa cgaagtgcgt cagcgtgctg
     6121 gttattaagg caatattttt tcagaggaac cgcaataccg tcggtattaa cggtttacgg
     6181 gtgatgaagt gtttagcctg catctttgcc gggtgcagtt ataagcattt cagtaacaga
     6241 accccggtgt tgttttccgg gattgaagga gaattgaatc atggcgttac cagcagaaag
     6301 ccttattgcg catacgctca gaaaaatgaa ggccagcatg gcagagaaag ccagtcgtga
     6361 ggagggaagg atacgcagtg ggctgctgtt taccggtaat gttcatgatg cggtgccccg
     6421 taggctgctg ctggacacgc gcctttctcc tcttgataaa acggcatgga tgatgattcg
     6481 tctgcatgcg ctgagtaatg agggcgcagt attccccacc tacgatgaac ttcagttgca
     6541 actggcctca cccggtagcg ggaaggcatc ccgtgaaacc gtaagccggg tactgaccat
     6601 gcttcggctg accggctggc tgagtctgtg taaacgggtt cgtgatgaaa aaggccgtat
     6661 ccgtggaaat atctacgccc tgcatgatga acctcttggc tgttgtgatg cagaaaagtt
     6721 tgaccctcgc tacttggata tggtgctgga ttctgcatgc aagaacagga acaagaccat
     6781 ccggtataca gccagagagg ttctcagtga tatcaaaaat gaacctggta tgcggcatta
     6841 ccacagccat atcacgctgt ttgaatcacg gatgggcgca gcgcagacac cgtctcagat
     6901 ggcagcgcgt cagcgggtaa atccggcggt gaatggtcgt gcagcgaaga gcggttcgga
     6961 atccgcacag ccccctgtat gtgcgtcggt aaccgaactc agtgctcagg taaccgaact
     7021 ctgtactgcg gtaaccgaac ccggagaaaa aacagaggta acgcaacgca gtacggattc
     7081 cgaacaacca ggaaaatcag tgggttatca ctcagtacgg aaaccgaacc actacgtacg
     7141 taatattaca cacagtatga aaaaaaatac gtacgtattg ccggatgcac tgaaatcaca
     7201 actcagcgtg gaagacaccg gcatgctgac gaagcaactg caggcgttgc cggaaacgct
     7261 ggcggaaggt gtgctggaca gtctgtcaca gatgctcagc aaaaatacgc ttcagaatcc
     7321 cgttggctgg atgctggctg tgatgaaacg tgcgcgtgaa gggaacttta actcgccgga
     7381 tatgagcaga agcggaacga tccagtctca gcctgcccgt ccatcatctc agtccggaca
     7441 tggatgtgat gaacgtgaac gtcgtcgcag ggaagtggct tcgccagaaa aagcgagaaa
     7501 tattgttgca gaattccgtg ccagcataca gcgtgctaat gctgcccgtc tggctatgcg
     7561 tggttaagtg ctggccggga tgtttcctgt tgtggcgaac actgttggta gtgccatcag
     7621 tggctaaaaa gtgaacggtg ttgccagtgg taacgctgat taaaaaatga gcgatactgc
     7681 cagtggcaat agtgattaaa aagtgaacgg cgttgccagt ggcaacagtg gttaaaaaac
     7741 gagcagtgtt ggcagtgcca acggtgacta aaaagtgaac ggtgttgcca gtggcaacag
     7801 tgattaaaaa gtgaacggtg ttgccagtgg caacgctgat taaaaaatga gcgatgttgc
     7861 cagtgacaac agtgattaaa aagcgagcgg tgttggcagt accaacagtg actaagaaat
     7921 gagcgctttt gccaatgcta ctgtgcgtaa tttcagtcat ctcttttttt attttcgatt
     7981 gtcagatctt tgtcggtgag aggactattt ctgtctgatg tcattttcaa tagtggagat
     8041 acaacatgcc agaagaaata gtgaaggagc cagcctctca tcgtgtggga gcttttacat
     8101 caactctgaa tatagagcta cattcccgct atgccattat gttatgggaa ggtcgacgtg
     8161 gctcaaaagc taaaaataat gaggggcgta aagtcccacg aattatgagt atgccagaag
     8221 ccattaaaat gactggtggt ctttaccggg attctcttgc tgataatcct tatgctgatg
     8281 agacgatgtg gaaactggag aatgcgatta ttgatgcgac tgaaaaaatt aatgccgaag
     8341 tagcccagct tgacgatatt ctcagaaaca taccttccgg gattactatc aaggatgtgc
     8401 tttcgcgttc accagtcaat ataggggtat tcagtcgttc gccgcttggt taccgttgtg
     8461 tctggcttct cgtcggatat gatcagcttg cattaaaaac gtttcaggcg gctcatctgg
     8521 ggttaatttc ccgaaaccag cgtgatgaac tgttgtcgtt tggtgcgtac cttatccgca
     8581 agatttacgg tattctacaa atctatcgtt ctgtaggtgt cacgcgggat gatattgaaa
     8641 ataaaaccag taaaggtgag aaggctattg ctcgttacgg tatgcctgag ccagatattc
     8701 tttctggtaa agctagatct tcattctctc cccctctgaa ccgtaagtca cgtaacttat
     8761 catagcagtg agttgccagg gggaattatg cgtctttttt tgtgtgagaa accttcacag
     8821 gccagtgaca ttgccagagt gcttggggct gccagacgcg gtgaaggcct gattgaaggt
     8881 aacgactttg ttgtgacatg ggcttttggt catttgctgg agcaggctcc accggaggcg
     8941 tacggtgagc agtatggcag tccctggagc attgaggttc tgccggttat tcctgaacag
     9001 tggcagatgg tggtgaagga taaggtcgcc aggcagttta atgttatcag gcgtcttctc
     9061 ggtaaggcag atgaagtagt gattgccacc gatgctgatc gggaaggcga aattattgcc
     9121 agagaactgc ttgattattg cggttataca ggagtagttc gacgcctctg gttatccgca
     9181 ctggtatcag ggaagcgctg ggcagaatac ttccaggaga ggaaactgtg cgtttatatg
     9241 aggccggtaa ggggcgcagc cgggcagact ggctgacggg gatcaacctg acgcgtttgt
     9301 acacgatgaa agccagggag tgcggcgtgt ctggtgttct ctcgattggc agggtgcaga
     9361 ctcccacgct tgcgatggtg gtcagacggg atgcggaaat taaaaatttt atcccggtac
     9421 cttactggca actgaacctc tggcttgaga aagacaacgt gcgttttcgc gcagcctggg
     9481 ttgcggcaga gcagtactgt gatgtggaaa agcggtgcgt gaatgtgcag gctgccacaa
     9541 gcgtccagtc gcagtgtcgt caggccggtg ttgctgtagt cagagaggtc agcagcaaaa
     9601 gagagaagac gccgcctccg ctttgttttt cactcggtac actgcaggag gtctgctcga
     9661 aaaaatttgg tttaggtgcg caggccgttc ttgatatagc ccagtcgttg tacgaaaaac
     9721 acaaggcaac gacgtatcct cgtactgact gtggttatct gccggtatca atgcataaag
     9781 aagtgccgga ggttctggcg gctgtggcaa acaccgatcc ggacattgcc cctgaactgg
     9841 caaaactcag tcctgatatt gtttccagag tctggaatga taagaagatc actgcgcatc
     9901 atgcgattat ccccacccgt caggcgttta atattggtgc cctgagtgat aacgaactca
     9961 gggtttaccg gttaatccgt cagcattatc tggctcagtt tttacccgtg caggaagcgg
    10021 acgtcactga agccactttt gttgttgccg ggcagttgtt tggcacgcga ggacgggtgg
    10081 aagtggtgaa aggatggcgt gcgcttttta ttgcagatgc gccgcatgat gaagacgagg
    10141 ggggcgatac agagcagaca ctgcctctgc ctgttctggc taagggggat gaatgtcgtc
    10201 ttgttgaagc agaactgaaa gagatgaaaa ccacgcctcc gcgtcactat acggagggca
    10261 cgctcattgc tgcgatgaaa aatgcggcgg gaatggtgag cgatccggct ctgaaaaaag
    10321 tgttacgcga gaatgccgga ctgggcacgg aagcaacccg tgctgaaata ctggagacac
    10381 tgttcacccg caaatatatt gagaaacagg gtaagcatat ccgggcaaca caactggggg
    10441 cagagttgat agcaggatta cctgaggtgc tgaccagtcc gggaaccacg gcactttggg
    10501 agcaggccct ggatgatatt gcacagggta aactgtcgct ggcgtcattt atggagaagc
    10561 agaaacagtg gctgaatcac ctcgtctcgg gagcccgacg gcagcaattg cagattacgg
    10621 caccagtgac gccggaatgc ccactttgcc agggcagaat gcggaagctg aaaggtaaga
    10681 acggagagtt ctggagttgc cagcgttacc cggagtgtaa aggcgttatt tcagagcaga
    10741 aaacgtcaca cagtaaacgc ggcggcagaa aaattaaaaa gcgtggatga tgattacttc
    10801 ctttttttta tttgttaaag tgcatacgct ttgatgtata cccccatgat cagtgggaaa
    10861 cagagcagcg attaaacaac ggcgttgttt tgtttctgat ccgcaacggc cagcgtccgg
    10921 ttgagttgca aggcgtatga actccgtgaa aaccgcgccg ggagtgatcc ccggagacaa
    10981 gtgagatgac cgaagggctg gtaaccccat gacggcgctg aagattcagc gcctttttta
    11041 tgctcatttc aaagagtgaa catctcggtg agataattat ttaatgacgt tttgttcttg
    11101 actttgtgtt caggtgtttt aaaaatgagc atgctaaatc gctgccttgc agccaaccaa
    11161 tgtcctggtg atctgagaag gacgccttcg ggtgattgca atatcctcgt agcctgaaag
    11221 aggatgcctt tatggcggta catccgttta acctctggga acggatatca ccacagttat
    11281 ggtgatcgag gtatgtttaa ctgcacagac agccggaaat acagctgatt ccggcccccc
    11341 gacgggaact atcacacccg tcaggggcgg tggtgttctc cgtcatatct ttttttatcc
    11401 atggagaaca catcatgtct gcaacaaata cttcttctgc aaaagctgaa tacttcaatc
    11461 tgaccgtaaa aggtctgggg tacctcagta atgtgtgtca ggttaaccat cagaatggct
    11521 cgttcctttg ctgcgtgatt aacgcactga gtggaccaac ggacaatccg tcctatacgc
    11581 gttttgatgt tacggttgtt ggtaaagatg caaccaccct gatctctcgc tgccagaaag
    11641 cagtggatga ggaccgtaag gttttgcttg gctttgcgtt aagcaacctg gccaccgata
    11701 tcttcaccct gaacaaggga gagcatgccg gtgagcagcg cgtcagtttg aaagcccgcc
    11761 tgatcaaggt tgactggatt aaaattggcc aggagcaggt ctataaggct gaaaaagcgg
    11821 agtccctgcc accgcaaaat ggcacaacta cacgtcaata cgcagaaaac tctttctgat
    11881 cgtatttccc tcagcctccc tgatgatgaa aacagaccac catcagggag gtctgttttc
    11941 ttatcgtttt tgcaaacttt aaggaaacac atatgaaccg tcaaaacggc attcttgcag
    12001 ctgcggctgg ctatacatta cccctggaaa tcctcaaaag tacgcgtgga tattatattg
    12061 gcactcagtg ttctgagggg ccggtgtccc gtgaatctga agagtatttc aaaaaacatg
    12121 atcaggctga gcaggcgctg aaaaatggta cctggacaca gcgttatgac tggcaatgag
    12181 gggggagtat ggcttcacgt ggcgtaaaca aagtcatcct gcttgggcat ctgggacagg
    12241 atccggaggt caggtacttt cctaacggtg gtagtgcagt ggcgaatttg tcactggcga
    12301 ccagtgatac ctggaaggat aaacagagcg gtgaaatgaa ggaagtgacc gaatggcacc
    12361 gcattgttat ttatggaaaa ctggcagaaa tcgccg
//
LOCUS       DAZGJT010000119        12276 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZGJT010000119 DAZGJT010000000
VERSION     DAZGJT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.119"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..1410
                     /gene="allB"
                     /locus_tag="ACHKFK_004004"
     CDS             49..1410
                     /gene="allB"
                     /locus_tag="ACHKFK_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO9054234.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKDGKIAAIGQDLGDAKDVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEMGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHVSSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMKAWGG
                     IAGLQSCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            1466..2767
                     /locus_tag="ACHKFK_004005"
     CDS             1466..2767
                     /locus_tag="ACHKFK_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO9054235.1"
                     /translation="MFNFAVGRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGQGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            2789..3934
                     /gene="glxK"
                     /locus_tag="ACHKFK_004006"
     CDS             2789..3934
                     /gene="glxK"
                     /locus_tag="ACHKFK_004006"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO9054236.1"
                     /translation="MKIVIAPGSFKESLSAEKCCQAIKAGFSTIFPDAHYICLPIADG
                     GEGTVEAMVAATDGNIVKLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQA
                     LAANGGNLARVASIEMNECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQLTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(4063..4848)
                     /gene="allE"
                     /locus_tag="ACHKFK_004007"
     CDS             complement(4063..4848)
                     /gene="allE"
                     /locus_tag="ACHKFK_004007"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO9054237.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFT
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYIPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(4859..6094)
                     /gene="allC"
                     /locus_tag="ACHKFK_004008"
     CDS             complement(4859..6094)
                     /gene="allC"
                     /locus_tag="ACHKFK_004008"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO9054238.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMTASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDATVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(6116..7165)
                     /gene="allD"
                     /locus_tag="ACHKFK_004009"
     CDS             complement(6116..7165)
                     /gene="allD"
                     /locus_tag="ACHKFK_004009"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO9054239.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QKGVAVVGISRMGHSGAISYFVQQAARAGLIGISLCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELLRQHLSQTMRELNTITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            7482..9149
                     /locus_tag="ACHKFK_004010"
     CDS             7482..9149
                     /locus_tag="ACHKFK_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO9054240.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAVMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAETVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHQHGMMLDADGHQILDLGDDFYTVGRPHPMI
                     DPTLRNLLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            9159..10418
                     /locus_tag="ACHKFK_004011"
     CDS             9159..10418
                     /locus_tag="ACHKFK_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO9054241.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNKGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGASLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            10429..11244
                     /locus_tag="ACHKFK_004012"
     CDS             10429..11244
                     /locus_tag="ACHKFK_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO9054242.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYYLLRITPP
                     AHPQQLAVAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAAAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            11241..12134
                     /locus_tag="ACHKFK_004013"
     CDS             11241..12134
                     /locus_tag="ACHKFK_004013"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO9054243.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPHVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEATYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
BASE COUNT         2757 a         3044 c         3460 g         3015 t
ORIGIN      
        1 attgaacatt gagttaaaaa ccaatctgta ttttacaagg agtttgttat gtcttttgat
       61 ttaatcatta aaaacggcac cgttatttta gaaaacgaag ctcgcgttgt agatatcgcc
      121 gttaaagacg gaaaaattgc tgctatcggc caggatctgg gcgatgcaaa agacgttatg
      181 gatgcgtctg gtctggtggt ttcgccgggc atggttgatg cgcacaccca tatttctgaa
      241 ccgggtcgca gccactggga aggttatgaa accggtactc gcgcagcagc aaaaggtggt
      301 atcaccacca tgatcgaaat gccgctcaac cagctgcctg caacggttga ccgcgcctca
      361 attgaactga agttcgatgc cgctaaagga aagctgacta tcgatgcggc acaactcggt
      421 ggcctggtgt cttacaacat tgaccgtctg catgagttgg atgaaatggg tgttgtcggt
      481 ttcaaatgct tcgttgcgac ctgtggcgat cgcggtatcg acaacgactt ccgtgacgtc
      541 aatgactggc aattcttcaa aggcgcgcag aagctgggtg aactgggcca gccagtgctg
      601 gtgcactgcg aaaacgcgct gatctgtgac gcactgggcg aagaagcgaa gcgtgaaggt
      661 cgcgtaactg cccatgacta tgtggcttcg cgtccggtat ttaccgaagt ggaagcgatt
      721 cgccgcgtac tgtacctggc gaaagtcgct ggttgccgtc tgcacgtttg ccacgtcagc
      781 agcccggaag gtgttgagga agtgactcgt gcacgtcagg aaggtcagga tgttacttgt
      841 gaatcctgcc cgcattactt tgtgctggat accgatcagt tcgaagaaat cggtactctg
      901 gcgaagtgtt caccgccgat ccgcgatctg gaaaaccaga aaggcatgtg ggaaaaactg
      961 tttaacggtg aaatagactg cctggtttcc gaccactctc catgcccgcc ggaaatgaaa
     1021 gccggtaaca tcatgaaagc gtggggcggt atcgctggtc tgcaaagctg catggacgtg
     1081 atgttcgatg aagcggtaca gaaacgcgga atgtctctgc caatgttcgg caaattaatg
     1141 gcgactaacg cagcagatat tttcggtctg cagcaaaaag gccgtatcgc cccaggaaaa
     1201 gatgccgact tcgtcttcat tcagccgaat agcagctatg ttcttaccaa tgacgatctg
     1261 gaatatcgcc acaaagtcag cccgtatgtt ggccgtacta ttggcgcgcg tatcacgaaa
     1321 accatcttac gtggtgatgt gatttacgat atcgaacagg gcttccctgt tgcgccgaaa
     1381 ggtcaattta tccttaaaca tcagcagtaa tctggccctg caatgcccgt ccttgtggcg
     1441 ggcattctcc ggttaaggtg tgtttatgtt caattttgca gtgggccgcg aaagcctgtt
     1501 atcaggattt cagtggtttt tctttatttt ttgcaacacg gttgtggttc ctcctacgct
     1561 actttctgct tttcagttgc cgcaaagtag cctgcttacg ctcacgcaat atgcttttct
     1621 tgctaccgca ctggcctgct tcgctcaggc gttctgcggt catcgtcgcg ctattatgga
     1681 ggggcagggc ggcctgtggt ggggaaccat cctgactatc acccttggcg aagcatcgcg
     1741 cgggacaccg atcaacgata tcgctaccag tctggcggtg gggattgcgc tctccggcgt
     1801 gctgacgatg ttgattggtt ttagcggatt aggccatcgc ctggcacggt tatttacgcc
     1861 gtcggtgatg gtcttgttta tgttgatgct gggtgcgcaa ctgaccacta tctttttcaa
     1921 aggtatgctc gggctgccgt ttggcatagc cgacccgaat tttaaaattc aattaccgcc
     1981 gttcgcgctc tcggtggcgg tgatgtgcct ggtactggcg atgattattt tcctgccaca
     2041 acgttttgcc cgttacggcc tgctggtcgg tacgataacc ggctggttgt tgtggtattt
     2101 ttgctttcct tcttcgcatt cgctctccgg tgagttgcac tggcagtggt tcccgctcgg
     2161 cagtggcggt gctttgtcgc cggggattat tctgacggcg gtgattacag gtctggtaaa
     2221 tatcagcaat acctacggtg cgattcgggg cacggatgtt ttttatccgc agcagggcgc
     2281 ggggaatacg cgttatcgtc gcagctttgt ggcgaccgga tttatgacac tgataaccgt
     2341 gccgctggca gtaattccat tttcaccgtt tgtttcatcc attggtttat taacccagac
     2401 aggcgattac acgcggcgtt cgtttattta tggcagcgtt atttgcctgc tggtggcact
     2461 ggttccggca ctcacgcgac tgttttgcag tatcccttta cccgtgagta gtgcagtcat
     2521 gctggtttct tatctgcctt tactcttttc cgcgctggtg tttagccagc aaataacgtt
     2581 taccgctcgc aatatttatc gactcgcatt gccgttattt gtcggcatat ttttaatggc
     2641 attaccgcct gtgtatctgc aagaccttcc attaacgctt cgtcctctgc tcagtaacgg
     2701 cttactggtc ggaattttac tggctgttct tatggataat cttattccgt gggaacgcat
     2761 cgaataattt gttgaaaaag gattgataat gaagattgtc attgcgccag gctcttttaa
     2821 agagagctta agtgcagaaa aatgttgtca ggcaattaaa gccgggtttt cgaccatatt
     2881 tcccgatgcg cactatatct gtttgccgat agcggatggc ggcgaaggga cggtggaggc
     2941 gatggtcgcc gcgacggacg gtaacatcgt gaagcttgaa gtctgcgggc cgatgggcga
     3001 aaaagtgaat gctttttatg gccttaccgg cgacgggaaa acggcggtga ttgagatggc
     3061 agcagcaagt ggcctgatgc tggtcgcgcc tgaaaagcgt aatccgttgc tggcctccag
     3121 ttttggtacg ggggagttaa ttcgccatgc gctggataat ggcattcgcc atattattct
     3181 cggcattggc ggcagtgcga cggtcgacgg cggtatgggc atggcgcagg cgctcggtgt
     3241 gcgtttcctt gatgccgacg gtcaggcgct ggcggctaac ggcggcaatt tagcgcgcgt
     3301 ggcaagcatt gagatgaatg aatgcgatcc gcgtctggcg aactgtcata ttgaagtagc
     3361 atgtgacgtt gataacccgc tggtaggggc acgcggcgcg gcggcagtgt ttggcccgca
     3421 aaaaggggca acgccggaga tggtcgaaga gctggaacag gggctgcaaa attacgcccg
     3481 tgttttacaa cagctaactg aaatcaacgt ctgccagatg gctggtggcg gcgcggcggg
     3541 cggtatgggc attgcggcgg cggtattcct caatgcggat attaaaccgg gtattgagat
     3601 tgtgttgaat gcggtcaatc ttgcgcaggc agtgcagggt gcagcactgg tgatcaccgg
     3661 ggaagggcgc atcgactcgc aaacggcagg cggtaaagcg ccgctgggtg tggcatcggt
     3721 ggcgaagcag tttaatgtcc cggtgattgg gattgctggc gtattgggtg atggcgtgga
     3781 agtggtgcac cagtacggca ttgatgcggt attcagcatt ttgccacgtc tggcaccttt
     3841 agccgaagtg ctcgccagcg gtgaaaccaa tctcttcaac agtgcgcgaa atattgcctg
     3901 cgccattaaa ataggtcagg gaattaaaaa ctgactatta cctttaaagc ggatgcgatt
     3961 tatatcgcat ctttttttac ctgaattgac tacaaaaaaa gccacggtat aaaccgtggc
     4021 aaaatccaac atagctaaaa ataatcaggc gagtggtatg acttaaatct ctacgtcgcg
     4081 gttgcaatct ttcgagtaaa tatagctgaa cgcttcacca cgccctacgc cataaccagc
     4141 ctgtaaagaa taagcgccca taaagatgta atcgcctttt ttcaccggga tccagttatt
     4201 gtcgaggtta taaaccccct gaccggaaag aatataggca ccgtgttcct gaacgtgggt
     4261 ttcgatataa ccgtggctgg cacctggtgc aaaagagagg atatgcatgt tcatatcaaa
     4321 acctaactct ttgggcagaa aatccagcag aataacatcg tccatgcctt cataatgaat
     4381 gcgttccagt tcgctggcat tgccagaaac cagccacggc gcatgacctt ctaccggaat
     4441 atagcgacgc ttatataaaa agatttggct gtcttcggcc tgggcgttaa caaacgtcat
     4501 taaggagcct ggcgggcaat aaagatagcc accttcactt aaggtaaatg ttttcccttc
     4561 ggctttggca gtgatatttc cagagatcac atacaggaac gtttcaatgc cttcgccacc
     4621 gaagccctgt tggttgccgc cgttttgatg cagtgtgacc agataatcaa caaaagaggc
     4681 acccagcttt ggcgtggaga ggattgtcgc gtcacaattt tcaaagcccg gaataatatt
     4741 ttttaccaga ccgtctggtg ttaacagtgc gaaattaccg tgtttcacaa tcgcacggtt
     4801 agccagtaaa tcatcgcggt aaccggtgac gttatttaaa tatcccattt ataactcctt
     4861 atttctgcca ggcaagttga taaagcatga gtgccaacgt tttgacccct tcggcaaggt
     4921 cggtaatatt ggtgcgttcc gccgggttat ggctgatccc gttgatgctg ggaatgaaaa
     4981 tcatgcaggt cggcacgcga ggcgcgaaaa tttgcgcgtc gtgcccggca ccactgtgca
     5041 tcacccggta attcagtttt tcgctttcac acaattctgt cagggtggcg accaactcct
     5101 tattcatcgg cacgggttct tcgtccatcc ataaatcgat atcaatacca atatccattt
     5161 catcgcaaat cgcccgcatg tcgttttcta actgttgggt gaaatcgcgc agcacggtag
     5221 cgtcggtatg acgacaatca atggtgaacg tggttttacc cggcactaca ttcaccgtat
     5281 tcgggcgcgg ctctactttg ccaaaggtca gaaccagcgg gtcgcccatc tttttcgctt
     5341 tttcgacaga ctgatggcaa atgcgactga aagcgtaaac cgtatcacga cgataaccca
     5401 tcggcgtggt gcctgcatgg tttgattcgc cattcagcgt cacggtataa cgacgctgcc
     5461 cgacaattgc attcaccacg ccaattgatt gcccattact ttccagcaca cagccctgtt
     5521 cgatatgcag ttcgacaaag gctttaatat cctgacgcgg agttagtggg gcgttcggca
     5581 gagtaaatcc gcaagccttc atcgcatcga caaaactatt tcctttggca tcacagatat
     5641 tccgcacgtc gtcaggattc gccagcccaa aaatattttt actgccccag aaaacatacg
     5701 ggaagcggct gccttcttct tctgccatcg ccaccacttc gaccgtacgt aacggcgcgc
     5761 cgtattgcgt tttcagccag tcaatagcca gccacgccgc cagcgcaccg aattgcccgt
     5821 cgaggttacc gccgttaacc acggtatcga tatgcgaacc gctcagaacc acttcctgtg
     5881 gatattcggt gccactcagg cgaccgtata aattccccac ttcatcgaaa cgtgtttcca
     5941 gcccgcttgc tgtcattctt tttttaaact gctgctgggt ttccagccat tccggcgaat
     6001 aaagtaaacg ggtcatccca cccgctgggt cagcgccaaa agaggaaagc cagggcagcg
     6061 tttcttctat agcttgacgg aaatgtgtaa tcataagaaa gtcctgtctc aataattatt
     6121 gcgcaaaggg atttttcgtt tcgtatgacg tgttataaag cgcgtcggaa attaaatact
     6181 ggtaaatatc atcaacaatt tcgatgcctt cgacggcggc ttttcgttgt ttaatatcct
     6241 gatcctgtcc gggataataa acctgattaa aaccgggcgc gggggtaatg gtatttaatt
     6301 cgcgcatggt ctggctaaga tgttgacgga gtaattcgct ggaggagaaa aagttcgggt
     6361 taataactat atgtaattgc cccaaattac gcccggcgtg taaatcgtca tacatcgaac
     6421 taacctgtcg cccgaacggt aagccgagta agacgcctga gaggacgtca atcatcatca
     6481 tcaggccata ccctttcggc ccagcggcgg ggagcagagc atgtaccgcg aacggatcgg
     6541 ttgttggtgc accgttttta tcgactgccc aggtatccgg gatagacata ttgcgcgagc
     6601 gggcgtcgag cacttttccc catgcctgta cggtagtcgc catatcgaag gtaaggatct
     6661 catcgccttc tcccggcgcg gcaaaggcca gtgggttagt gccgtagtaa atttccgcgc
     6721 cgccaaacgg caccaccatt ggatcggact ggcacagcga aatgccaatc aatccggcgc
     6781 gggcagcctg ctgcacaaaa taagagattg cgccgctgtg acccatccgg ctgataccga
     6841 ccaccgcaac gcctttttgc tgggcggttt tgatggcatg ttccataccc attttcgccg
     6901 cgacctgtcc ggcggcattg tcggcatgta aaattgccga gcacggtccg gtttcctcaa
     6961 gacgaaactc cggttcgcgg ttggtgccgc cttttgaaat gcgttcggcg tagtattcaa
     7021 cgcgcaccgc gccatgagag tggatccctc tggcatcggc gtaaaccaat acttcagcca
     7081 cggttgcagc gtgctcacgt tttaacccag cctggcagag tttattctcg attagctggt
     7141 ggagtgtttc ccgactgatt ttcatctgtc ttccttttta acgacggtgt gaagcatgac
     7201 tgcaattaac atacagggaa aatatctgga ttatgtgatc cagacaggca aaaaaatata
     7261 gttagaattt atttgataat cttcgtgctt ttaacctggt ttttaaaaca accatgctta
     7321 ttaaaaaata acgcgtaata gtctggcggt tatttgaatt cttttgttaa taatccacgt
     7381 gtgatattca tcaccttatt tactcgttgt catcgatacc gtaatcgcca cattaacact
     7441 gctcgtgcaa ttgccatggg tgcaattttt aaggagttgt tatgatccac gcctttatta
     7501 aaaaagggtg ttttcaggat tcggtcagtt taatgattat ttcacgaaaa ctcagcgaat
     7561 cagaaaatgt tgatgatgtt tccgtaatga tgggtacgcc cgccaataaa gcgttattag
     7621 atactacagg tttctggcat gacgatttta ataatgccac gccgaacgat atttgcgtgg
     7681 caattcgtag cgaagcggcg gatgcgggga tcgcgcaggc ggttatgcag cagcttgaag
     7741 aggcgctaaa acaactggcg cagggttcag gcagcagcca ggcgttgacg caggtgcgtc
     7801 gctgggacag tgcctgtcag aaattacccg atgccaatct ggcgctgatt tcagtggctg
     7861 gtgagtatgc ggcggagctg gcgaaccagg cgctggatcg caacctcaac gtgatgatgt
     7921 tttccgataa cgtcacgctg gaagatgaaa tccaacttaa aacccgcgcg cgggaaaaag
     7981 gcttgctggt gatggggcca gactgcggta cgtcgatgat tgccgccacg ccgctggctt
     8041 ttgctaacgt gatgccggaa ggcaacattg gcgtcatagg cgcttccggt accgggattc
     8101 aggagctgtg ctcacagatt gcgctggcag gggagggaat tactcacgcg attggtcttg
     8161 gcgggcgcga tctcagccgt gaagtgggcg gcatcagtgc gctaacggcg ctggaaatgc
     8221 tcagtgcaga cgagaaaagc gaagtgctgg catttgtttc aaaaccacct gccgaaactg
     8281 tgcgtctgaa aattgttaat gccatgaaag caactggcaa accgacggtg gcgctgtttt
     8341 taggttatac cccggcggtg gcccgcgacg agaatgtctg gtttgcctcc tcgctggatg
     8401 aggccgctcg tctggcttgt ctgctttcac gcgtcactgc gcgacgtaac gcgatagcgc
     8461 ctgtcagcag cggatttatt tgcggtttgt ataccggcgg tacgctggct gctgaagcgg
     8521 cgggattact tgccggacac cttggcgtgg aagccgacga tacccatcaa catggcatga
     8581 tgctggacgc cgacggacat cagatcctcg acctgggcga tgatttttac accgtcgggc
     8641 gtccccatcc gatgatcgac ccgaccttac gcaacctgtt aattgccgat ctcggcgcta
     8701 aaccgcaagt gcgcgtgttg ctgcttgatg tcgtgattgg cttcggtgcg accgccgatc
     8761 ctgccgcctc gctggtgagc gcctggcaaa aagcctgtgc cgcgcgttca gacaatcaac
     8821 cactgtatgc cattgccacg gtgacaggca ctgaacgtga cccgcaatgc cgctcgcagc
     8881 aaatcgccac gctggaagat gcggggatcg cggtagtgag ttcgctaccg gaagccacct
     8941 tgctggctgc ggcgttaatt catccgctct cgtcagccac acagcaacac acaccgtcat
     9001 tactggaaaa cgtcgccgtg attaacatcg gattacgcag ctttgcgctg gagctacaaa
     9061 gcgccagcaa accggttgtg cattaccaat ggtcgccagt cgccggcggc aataaaaaac
     9121 tggctcgttt attagaacgt ttgcaataag gggttcccat gtttacatca gtggcgcaag
     9181 ccaatgctgc ggttatcgaa caaattcgtc gcgctcgtcc acactggctg gatgtgcaac
     9241 cggcttcttc acttatcagc gaactaaaca agggcaaaac actgcttcac gccgggccgc
     9301 caatgcgctg gcaggagatg accggaccca tgaaaggggc gtgcgtgggc gcatgtctgt
     9361 tcgaaggctg ggcgaaagat gaagcgcagg cgctggcaat actggagcag ggggaagtga
     9421 acttcattcc ttgtcaccat gtgaatgccg tcgggccaat gggtggcatt acttctgcca
     9481 gtatgccgat gctggtggtc gagaacgtga ccgacggcaa ccgggcgtac tgcaacctca
     9541 acgaaggtat cggcaaagtg atgcgttttg gcgcttacgg cgaagatgtc ctgactcgcc
     9601 atcgctggat gcgcgatgtg ttaatgccag tattaagcgc ggcgctgggg cgcatggagc
     9661 gcggtatcga tctcacggca atgatggcgc agggcattac catgggcgat gagttccatc
     9721 aacgcaatat tgcttcctct gcactgttaa tgcgtgcgct ggccccacaa attgctcgcc
     9781 tcgatcatga taaacagcac atcgccgaag tgatggattt cctcagcgtg accgatcagt
     9841 tcttcctcaa tctcgcgatg gcctactgca aggcggcgat ggatgcaggc gcgatgatcc
     9901 gcgcaggcag catcgttacg gctatgaccc gcaacggcaa tatgttcggg attcgggtaa
     9961 gcgggctggg tgaacgctgg tttactgcgc ctgtaaacac tccgcaaggt ctgtttttca
    10021 ccggcttctc gcaggagcag gcgaacccgg atatgggcga tagcgcgatt accgaaacct
    10081 ttggtatcgg aggtgcggca atgatcgccg cacctggcgt aacgcgcttt gtcggtgcgg
    10141 gtggcatgga agcggccaga gcggtatctg aagagatggc ggaaatttac cttgaacgca
    10201 atatgcaatt gcagatcccc ggctgggatt tccagggggc gagcctgggg ctggacattc
    10261 gtcgcgtggt agaaaccggc attacgccac tcatcaatac cggtatcgcc cataaagagg
    10321 cggggatcgg gcagattggc gcaggcaccg tgcgggcacc gctggcgtgc tttgaacagg
    10381 cgctggaagc actggctgaa agcatgggga ttggttgagg aacgcacaat gacgatcatc
    10441 catcctctac ttgccagccg tagcgcaccg aattatcgcc agtcctggcg gttagcggga
    10501 gtgtggcggc gggcgattaa cctgatgacg gaaagcggcg aactgttaac gctgcatcgt
    10561 caggggagtg gtttcggccc cggcggatgg gtgcttcgcc gcgcgcaatt cgatgcgtta
    10621 tgcggtggat tatgcggcaa tgaacgacca caggttgtgg cacaagggat tcgcctcggg
    10681 cgtttcacgg ttaaacaacc acagcgttat tatctgctgc gtattacgcc gcctgcgcat
    10741 cctcaacagc ttgccgttgc atggatgcaa cgcgcggagg aaaccggact tttcgggcca
    10801 ctggcattgg cggcaagcga tcctctgcct gctgaattac gccagtttcg tcactgtttt
    10861 caggccgcgc tcaatggcgt taagaccgac tggcggcact ggctgggtaa aggccccgga
    10921 ttaacgccaa gtcatgatga cacgctgagc ggaatgctgc tggcggcctg gtattatggc
    10981 gctttagatg cgcgctccgg tcgcccgttt tttgcctgtt ccgacaatct tcaactcgtt
    11041 accactgcgg tgagcgtcag ttatttacgt tatgccgcgc aaggatattt cgcctcgcca
    11101 ctcctgcact ttgttcatgc tctgagttgc ccgaaacgta ccgcagcggc gatcgattcg
    11161 ctgctggcgc tggggcatac gtcaggggca gatacgctgc tggggttctg gcttggccaa
    11221 caattattac aagggaaacc atgaaaacac tggttgtggc tcttgggggc aacgccttac
    11281 tccagcgcgg tgaggcgctg acggcagaaa atcaatatcg caatattgcc agtgctgtgc
    11341 ccgcgctggc gcgcctggcc cgttcttatc ggctggcgat tgttcacggc aacgggccgc
    11401 aggtggggct gctggcgtta cagaatctgg cgtggaaaga ggtagaaccg tatccgctgg
    11461 atgtactggt ggcggaaagc caggggatga ttggctatat gctggcgcag agcttgagcg
    11521 cacagccgca gatgccgcat gtcacgacgg tgctgacgcg cattgaggtt tcgcctgatg
    11581 atccggcgtt tttgcagcca gagaagttta ttggtccggt ttatcagcca gaagaacaag
    11641 aggcactgga agcgacttac ggctggcaga tgaaacgtga tggtaaatat ttgcgccggg
    11701 tggtggcgtc tccgcaaccg cgtaaaattc tcgacagcga agccatcgag ttgttgctca
    11761 aagaggggca tgtggtgatt tgcagtggcg gcggcggtgt gcctgtgacg gaagacgggg
    11821 cagggagtga agcggtgatt gataaagatc tcgccgctgc gttgctcgcc gagcagatta
    11881 atgccgatgg actggtgatc ctcaccgatg ctgatgcggt atatgaaaac tggggaacgc
    11941 cgcagcaacg tgccattcgc catgccacac cggatgagtt agcgccattt gccaaagccg
    12001 atggttcgat ggggccgaag gtaacggcgg tgagtggcta tgtcagaagc cgtggtaaac
    12061 ccgcgtggat tggggcgtta tcgcggattg aagagacgct ggcgggcgaa gcggggactt
    12121 gtatttcgtt gtagtgatgg gcaccaggca attggcacat ccagcctgat gcgatgctgg
    12181 cgcgtcttat caggtctaca gtcggtgttg gagcggtggg ccggataagg cgtttacgcc
    12241 gcgtccggca atcggcacac catgcctgat gcgatg
//
LOCUS       DAZGJT010000120        12275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZGJT010000120 DAZGJT010000000
VERSION     DAZGJT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.120"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..318
                     /locus_tag="ACHKFK_004014"
     CDS             <1..318
                     /locus_tag="ACHKFK_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9054244.1"
                     /translation="FERVWREYPHRAGSNPKRAAFNSWKARLREGVSPDAVLDGVRRY
                     SRYLEATGKAGTEFVQQASTFFGPNRNFENPWSLPKAGAVSQRCVNHISEPDTEIPPG
                     FRG"
     gene            325..1065
                     /locus_tag="ACHKFK_004015"
     CDS             325..1065
                     /locus_tag="ACHKFK_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9054245.1"
                     /translation="MKNISTGGVLERVRRLAPPHVTAPFRTTDEWREWQLAEGRKRSE
                     EINRLNHQTRVEKILNRSGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVILNQIVDRRTASLRTVGILTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            1091..1861
                     /locus_tag="ACHKFK_004016"
     CDS             1091..1861
                     /locus_tag="ACHKFK_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9054246.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESGVTEGQALQPEAPDAVTEEVAPKVTADMMIEFIAQKGATTCEEL
                     ADKFGVTTRKVASTLAVVTATGRLVRVSIKGRFHYKKPDVENGDLSASSSQDEQSHHE
                     AENSAAGDEMNRFPLASESTAKASTDEFIREVPSFTEKSASSVMLPPLHAASRELRRA
                     KKQVQKWERVCAALRELNKHREIVMQITDSSSSIASEK"
     gene            1876..2319
                     /locus_tag="ACHKFK_004017"
     CDS             1876..2319
                     /locus_tag="ACHKFK_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO9054247.1"
                     /translation="MAKVFTPEQREELKARIVELVRLNGRGTFRQLATETGVSKTAVS
                     RLSRELVVSGAIFDSGYGLFPSEQARKDWQNARKKLSRAKSKKPLVVDPGLIRALPDG
                     EIRRYDRRYNIICRECRKSEVMQRVLTFYRRCVQENNSLRSRTLT"
     gene            2333..2833
                     /locus_tag="ACHKFK_004018"
     CDS             2333..2833
                     /locus_tag="ACHKFK_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054248.1"
                     /translation="MHLNKEHQAMMGKRQTEEAVRDLEKLLGNRETKLLFLVPVIRQK
                     VEEILEENGIPKEYMTDAMEYSLELVRITPDLTWLDEYQQQFEKGAGTLLSVADKTEQ
                     KTEPSLFWNTVEAIARSKMFEFNNINHSGRDTHQSSHLEGVSMLLALLREQGECPQST
                     LNDTEQ"
     gene            3135..3392
                     /locus_tag="ACHKFK_004019"
     CDS             3135..3392
                     /locus_tag="ACHKFK_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054249.1"
                     /translation="MNNKINIPLEIKEINEIIEVTDRPEFTLMRRYETGTDEQKYIMV
                     AALAVMAIERERMEKDVMVIPARNDSPDLRWQEPKWDVAIR"
     gene            complement(3443..3748)
                     /locus_tag="ACHKFK_004020"
     CDS             complement(3443..3748)
                     /locus_tag="ACHKFK_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9054250.1"
                     /translation="MTMFNPPHPGEIIADILEGQNIGSRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3759..4079)
                     /locus_tag="ACHKFK_004021"
     CDS             complement(3759..4079)
                     /locus_tag="ACHKFK_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9054251.1"
                     /translation="MSKNYRSDALASVHEMMESLHDIGAVTKQTMREFDELCLQPAPS
                     MSPERIRALREREHLSQPVFARYMNVSKNLISDWERGVKRPGGPALRLLSVIEKNGIQ
                     AINV"
     gene            complement(4072..4443)
                     /locus_tag="ACHKFK_004022"
     CDS             complement(4072..4443)
                     /locus_tag="ACHKFK_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9054252.1"
                     /translation="MRIFKNAWFERFARKNKISDQSLREIVKQADNGLISANLGNGVI
                     KQRLPRVGGGKSGGYRTIIFYRVGMRAFFVYAYAKNERENITATEESTFRKAAPHVLN
                     LTDEQLAQLILQGQFTEVPNE"
     gene            4785..5459
                     /locus_tag="ACHKFK_004023"
     CDS             4785..5459
                     /locus_tag="ACHKFK_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO9054253.1"
                     /translation="MTVQISVKNLSPVTYNQIPVITTELLACLYGTETIRIQQNHHEN
                     KSRFIEGKHFFKAVGDELRNLRLVLNESQNEVRISPKTRSLILWTERGAARHAKMLET
                     DQAWEVFEKLEDCYFSQGKTTQTEQQPQIQPQFTAEEIILLCYMLVWMEKAQDLSKHL
                     YPIMKELNSSYTNKLYDIAFETIYMVTKNRDALLREVTRLDMSSSVIQRAMPMLKSLR
                     ARQFEF"
     gene            5514..5924
                     /locus_tag="ACHKFK_004024"
     CDS             5514..5924
                     /locus_tag="ACHKFK_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9054254.1"
                     /translation="MNQTIFLRSKQQQQFAINAILATTLDKDKPVTIRITDYKRNLAQ
                     NAKFHAMVADISRQVKWCGRWLKPEQWKVLLISGHAVATKQKADVLPGLEGEYVNIRE
                     SSAQMSVKRMASLIEYTTSWAVDQGVRFTDRRYE"
     gene            5921..6111
                     /locus_tag="ACHKFK_004025"
                     /pseudo
     CDS             5921..6111
                     /locus_tag="ACHKFK_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001254268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
     gene            6135..6425
                     /locus_tag="ACHKFK_004026"
     CDS             6135..6425
                     /locus_tag="ACHKFK_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000002235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9054255.1"
                     /translation="MTNLRKEARGRECQVRIYGVCNGNPETTVLAHYRMAGICGTGTK
                     PDDLLAAWACSDCHNEIDRRTRILDNNDARLYHLEGVIRTLAILLKEGKIKS"
     gene            6422..6784
                     /locus_tag="ACHKFK_004027"
     CDS             6422..6784
                     /locus_tag="ACHKFK_004027"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9054256.1"
                     /translation="MNEYEFVLPWPPTVNTYWRRRGSQYYISDKGQKYRKDVQQIIRQ
                     LRLDIFTKSRLRITIIAEPPDSRRRDLDNILKGLLDSLIHAGFAEDDVQFDDIRVIRG
                     VKVLGGRVGIKITELENI"
     gene            6781..6981
                     /locus_tag="ACHKFK_004028"
     CDS             6781..6981
                     /locus_tag="ACHKFK_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HFO9054257.1"
                     /translation="MNATIQTIPELLIQARGNMTEVSRKLNCNRATVRKYVGDKEGKR
                     HAVVNGVLMVHRGWDKGKATDA"
     gene            6974..7354
                     /locus_tag="ACHKFK_004029"
     CDS             6974..7354
                     /locus_tag="ACHKFK_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9054258.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVSWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHELLVDCYVRGMTFMSLARKHECSDGTIGKRLQKA
                     EGVIEGMLMMLDVRLEMDRYVQRL"
     gene            7489..8082
                     /locus_tag="ACHKFK_004030"
     CDS             7489..8082
                     /locus_tag="ACHKFK_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054259.1"
                     /translation="MDIEDFLEAEDNDLSLWSDDILSIDWFVILKELLKYQNRLKLNY
                     SELLLLANFVSFHQDVDSQTLPSISLFATRMRTSRDAIQGVLVCLEEKDMLQKISYAQ
                     IDHDDDLRNIYDIKPLIRRLIWLLKSGCDKKHTCPLCGKVAISNEEIEKKFGFRICGN
                     KKRPQSWCRSCRSPKQRRLNTLLCGKKRARKSSPEVV"
     gene            complement(9051..9123)
                     /locus_tag="ACHKFK_004031"
                     /pseudo
     CDS             complement(9051..9123)
                     /locus_tag="ACHKFK_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9410..11383
                     /locus_tag="ACHKFK_004032"
     CDS             9410..11383
                     /locus_tag="ACHKFK_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9054260.1"
                     /translation="MAFKHYDVVRASSPSDLAGKLTQKLKEGWQPYGSPIISNAGYGA
                     EFIQAIVAEGDMTSPVVSPGGDGTRTVVVEPEYYFVIALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACAYNDIIPADHCLHDVQDLSRFSHPKASAEQYGCVG
                     QGLHIAKKLLPFMPENAGILLVPCCRGGSAFTAGADGTFSESSGASDKSERWGVDKPL
                     YKDLLTRTQAALKANPKNILLCVVWMQGEFDLKQGAYASQPAMFDAMVEKYRSDLAGV
                     SGQCAGASPSLVPWICGDTTYYWKETYSAQYDAVYGAYKTKAGKNIFFVPFMMDDNGQ
                     KVGTNEPSEDPDIPAIGYYGSGGRTDAKSWTTSDRKTHFGSWARRGIISDRLATAILL
                     HVGRVADFVSGKVTKAAVSSGGGVSQPAQESGDTQPEAVVPTKIQSVLAYDVNAENAA
                     LASQGWTLTDVKNTLVSDRGSGKKALRLEKPENGLAQKKTWKVSHAIDAGKGKELFDN
                     GGEITLRFKIPDDVSLNASANQYSAGIYWRGSGWPGAESEEGYIAAFYLQTDKTNINV
                     MYHANTTSQRLGSYGPFDHDWHTLTFRFPGGGSLNVTPVLDNAAGKPFTLTRWTNAAF
                     EANALAITDITGNAATYPVLIESLTVGVNAVAA"
     gene            11525..11719
                     /locus_tag="ACHKFK_004033"
     CDS             11525..11719
                     /locus_tag="ACHKFK_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9054261.1"
                     /translation="MTFINQLMLYFCTVVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SATQSAGAKTEAPLIPEQPS"
     gene            11745..12035
                     /locus_tag="ACHKFK_004034"
     CDS             11745..12035
                     /locus_tag="ACHKFK_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9054262.1"
                     /translation="MTDIKQLVTAEAVKEVIRSEEVRSVLKQKLRQNLEERLDAEVDS
                     ILDELLGAQPEPSPELLPEPQAEDVTTENGDIQPESPVTDMTDTQPEPGTML"
     gene            12112..>12275
                     /locus_tag="ACHKFK_004035"
     CDS             12112..>12275
                     /locus_tag="ACHKFK_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO9054263.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVTPSQWAAIGVLGS
                     LCLGFLTYLT"
BASE COUNT         3373 a         2768 c         3213 g         2921 t
ORIGIN      
        1 ttcgaacggg tctggcgtga atatcctcat cgggcagggt caaacccgaa gagagccgca
       61 ttcaattcct ggaaggccag attacgcgag ggggtgtcac cggatgccgt gctggatggc
      121 gtgaggcgtt actcaagata cctggaggct accgggaaag cgggaactga atttgttcag
      181 caggcatcga cgttttttgg tccgaacagg aatttcgaaa atccgtggtc gctgcctaag
      241 gctggcgcag tcagccagcg ttgcgtgaat cacatttctg aaccggatac tgaaattccg
      301 ccgggtttca gggggtaatc agccatgaaa aacatttcga ctggaggggt tcttgaacga
      361 gtgcgccgcc tggcaccacc gcacgtgaca gcaccgttcc ggacgaccga cgagtggcgg
      421 gaatggcaac tggctgaggg ccgtaagcgc agcgaggaaa ttaaccgcct gaatcaccag
      481 acgcgggttg aaaaaatcct gaaccgttcg ggtattcagc cgcttcaccg aaagtgttca
      541 ttcgggaact accgggtgca gaacgacgga cagcgccacg ctctgagcca ggcgaaatcc
      601 atcgctgacg aactgatgac cgggtgtaca aacttcgtgt tcagcggtaa acctggtacc
      661 ggtaaaaatc acctggcagc agcgattggc aaccggctga tggcgaaggg gagaagcgtg
      721 attatcgtca ccgtgtccga tgtcatgagc gtgttgcatg acagctacga caacggccag
      781 tccggggaaa aatttttaca ggagctttgt ggagttgacc ttctggtcct tgatgaaatt
      841 ggcatgcagc gggatacgcg caacgagcag gtcatactga accagatcgt tgaccgcaga
      901 acggcatcac tgcgcactgt cgggatcctg acgaacctga atcacgcagc gatgagcaca
      961 ctcctcggcg atcgggtgat ggaccgtatg accatgaatg gtggtcgttg ggtgaatttt
     1021 aactgggaga gctggcggtc aaacgttgga cgtcagggta tgtgagaatt tttgacgagg
     1081 taaattttcg atggaaaccg tattgcatgc actgaaagcg atgggaaaag ccaattctgt
     1141 tgaactggcg gcgcggcttg atatcagccg tgaagaagtt ctcaacgaac tgtgggaact
     1201 caaaaaaaat ggcgttgttg ataaaacggg tcacacctgg tttctggctg tcgaaggtga
     1261 atccggggta accgaagggc aggcactaca acctgaagcg ccggatgcgg taaccgaaga
     1321 ggtcgctcca aaagttaccg ctgacatgat gattgagttt atcgctcaga agggggctac
     1381 aacctgtgag gaactggcgg ataagttcgg agttaccact cgtaaggttg cttccacgtt
     1441 ggcggtggta acagcaacgg ggcgcctggt acgtgtctcc atcaagggaa gattccatta
     1501 caaaaagcct gacgtcgaaa acggcgattt gagcgcgtca tcatcgcagg atgaacaaag
     1561 ccaccatgaa gcagaaaaca gcgcggcagg cgatgaaatg aatcgttttc cgttggcatc
     1621 agagagcacg gcaaaggcca gtacagatga gtttatccgg gaagttccat cattcaccga
     1681 aaaatctgcc agttcggtga tgcttccacc gctgcatgct gccagtcgag aacttcgtcg
     1741 cgccaaaaaa caggtccaga agtgggagcg tgtttgtgcc gcactacgtg agctgaataa
     1801 acaccgggaa attgtcatgc agattaccga ttcatccagc agcattgcat cagaaaagtg
     1861 ataactggag agcttatggc aaaagtattt acacccgaac agcgggaaga actgaaggcg
     1921 cgtattgtgg agctcgtgcg cctgaatggt cggggaacgt tcaggcaact ggctactgaa
     1981 accggagtca gcaagacagc tgtcagccgt ttatccagag aactggttgt cagtggcgct
     2041 atattcgact caggttatgg tttattcccg tctgagcagg cgcgtaagga ctggcaaaac
     2101 gcccgcaaaa aactctcacg agcaaagtcg aagaaaccgc ttgtcgttga tcccggtctt
     2161 atccgggcat tacctgatgg ggaaatccgg cgctacgaca gacgctacaa cataatttgt
     2221 cgagagtgcc ggaagagcga agttatgcag cgtgtactga cattctatag acgttgcgta
     2281 caggaaaata acagtctgcg tagccgcaca ttaacttaac gaggagctaa acatgcattt
     2341 gaataaagag catcaagcta tgatgggcaa gcggcagaca gaagaggctg tgcgcgatct
     2401 ggaaaaacta ctcggtaatc gtgaaacaaa actgcttttc ctggttccgg ttattcgcca
     2461 gaaggttgag gaaattcttg aagaaaatgg catcccgaaa gaatacatga cggatgccat
     2521 ggaatattca ctcgaactgg tacgaattac tcctgatctg acctggcttg atgagtatca
     2581 acaacaattt gagaaaggcg caggtacgtt gctttctgtt gctgataaaa cagagcagaa
     2641 aacagaacca tcgctattct ggaataccgt tgaggctatt gccaggagca agatgtttga
     2701 gtttaacaat atcaatcatt ccgggagaga cactcatcaa tcatcacatc ttgagggggt
     2761 gtccatgctt ctagctctgt tgcgagagca aggcgaatgt cctcagtcga cattgaacga
     2821 cactgagcaa tagtccatcc gtattttccg gcaagataaa gatatattgc gtcaaagcca
     2881 taaatagtcc ttggataccc ctctttaata gccagagagt ccccgaagca ttcaagaaag
     2941 taatttgagc gggctgtttc gtaatgaaaa ttccagcgag cctgattgag tttttttcca
     3001 tttatcctcc attgttgctc tgttttgaga aatggagacc ttcacgctgc aacgtgaggt
     3061 cgtgcgccgg acacgggtaa acatccggta ttcaaaattt aatgttaacg aatttttaat
     3121 attgaggtta tgacgtgaac aacaaaatca atattcctct cgaaattaaa gaaattaacg
     3181 aaatcattga ggttactgat cgccccgagt ttaccctcat gcgtcgttat gaaacaggaa
     3241 ccgacgaaca aaaatacatc atggttgcag cattggctgt gatggcgata gaaagggagc
     3301 ggatggaaaa ggacgtaatg gttattccgg cgagaaatga ttcaccggat ttgagatggc
     3361 aggagccgaa atgggatgtg gcaatccggt gattgatatt tctctggggg agggaaacgc
     3421 cgccagaacg acggcgcggt ggttaagcat gaggcgaaat ttctgccggg cggtattttg
     3481 ttgagagatg agatacgtca acttctcttt ccgctttttc aaggctccat gccgtctgga
     3541 gacgaatcca tgaagacgga gttccaccca ggacagcagc gagacgaaca gccatttcag
     3601 gagatattgt cgcattacct gaaaccagtc gctgaacagt ggaaggcgcg acatcaagcg
     3661 ctcttgccag ttctctgctc ccaatattct gaccttccag aatatcagca ataatttctc
     3721 cgggatgagg gggattaaac atcgtcattt agtggtaatc aaacatttat ggcctgaatc
     3781 ccgttttttt caataacgga aagaagtcgc agggcgggac caccagggcg tttcactccg
     3841 cgttcccagt cagatatcag gtttttactg acattcatat atctggcaaa gacgggttgt
     3901 gacagatgtt cacgctcacg gagtgcccgg attctttcag gagacattga aggtgctggt
     3961 tgcaggcaga gttcatcgaa ttctcgcata gtctgtttcg ttactgcgcc gatatcatga
     4021 agcgattcca tcatttcatg tacggatgca agcgcatcac tgcggtaatt tttactcatt
     4081 tggtacctcc gtaaactgac cctgtagaat cagttgtgcc agttgttcat ctgttagatt
     4141 gagtacgtga ggtgcagcct tccgaaatgt gctttcctca gtagcggtta tattttcacg
     4201 ttcatttttt gcatatgcat atacgaaaaa tgccctcata ccgacacgat agaaaatgat
     4261 tgtgcgataa ccgccagatt tgccgccacc gactcgtggc agacgttgct tgataacgcc
     4321 gttacccagg tttgctgaga taagcccgtt atcagcctgt ttaacaattt cgcgcagtga
     4381 ctggtcggat attttgtttt tgcgtgcaaa tcgctcaaac caagcgtttt taaaaatgcg
     4441 cattttttgt tccgttatta atgtataaca catagtatta cattgtgtag ggatctgcaa
     4501 tgccatcagt ttgacaacgt cgcgtatccg ggattatatt catcgcacgc cagcaaaatc
     4561 tggcgtcggg attggaaccc cggatatcga aacggtgcat aaccgcgctg gcggtttttt
     4621 tatgcgctaa gcacagtcac attcgcgatt tatggtgggc tgtgtggggg caccgaaagg
     4681 tgcgccggat gtttcgaccg gtagttccaa ccctgcacag tccgccaccc gatgattgga
     4741 acctgacggt ggtgaggtaa aaattatcga aacgcgaggt cgttatgact gttcaaatct
     4801 ctgtcaaaaa cctttcccct gttacctata accagattcc tgtaatcaca actgagttac
     4861 tcgcctgcct ttatggaaca gaaacaatcc gtatacagca gaatcaccat gaaaataaat
     4921 ctcggtttat tgagggaaag cacttcttta aagctgttgg tgatgaactc agaaatttgc
     4981 gactagtttt aaacgagtcg caaaatgagg tgagaatctc tcctaaaact cgctccctca
     5041 tcctctggac agaacgcggt gctgcccgtc atgcaaaaat gctcgaaacc gatcaggcgt
     5101 gggaagtgtt cgaaaaactg gaagactgct atttcagcca gggaaaaaca acacaaaccg
     5161 aacagcaacc gcagattcag ccacaattca cagccgaaga aatcatcctc ctctgctaca
     5221 tgctggtatg gatggaaaaa gcccaggacc tcagcaaaca cctgtacccc ataatgaaag
     5281 agctgaactc ctcatacacg aacaagctgt atgacattgc gtttgagact atctacatgg
     5341 tgacgaagaa cagagacgcg ctactaaggg aggtaacacg tctcgacatg tcaagttccg
     5401 ttatccagcg ggccatgcca atgctgaaaa gcctgcgggc aagacaattt gaattctgaa
     5461 actaaaggag cttcggctcc ttttttgttg ggaaaatcca gtgagaggga ataatgaacc
     5521 agactatctt cctccgaagt aagcagcagc aacaattcgc cattaacgcc atccttgcaa
     5581 caactctcga taaagacaaa cccgttacga tccgtatcac cgattacaag cgtaatctcg
     5641 ctcaaaatgc aaaatttcac gcgatggtcg ctgatatatc ccgccaggtt aagtggtgcg
     5701 gcaggtggct aaaaccagaa cagtggaaag ttttgttaat cagcggtcat gccgtggcga
     5761 caaaacagaa agctgatgtt ttgccaggtc ttgaaggtga atacgtcaat atccgcgaaa
     5821 gcagtgcgca aatgagcgtg aagcgtatgg caagcctgat tgagtacaca acatcctggg
     5881 ccgtggatca gggtgtcaga tttaccgaca ggaggtacga atgagacgac agcgacgaag
     5941 catcaccgat atcgtctgtg aaaactgcaa ttaccttcca accaaacgct cccgaaataa
     6001 acccaggcca gtcccaaaga atctgatgtt aaaaccttca actatacggc tcacctgtgg
     6061 gatatccggt ggctaaggcg tcgagcaaga tttaattgtc actccggata gcctactgca
     6121 tgaggaataa gatgatgaca aacctacgca aagaagcgcg cggcagagaa tgccaggtac
     6181 ggatttatgg cgtatgcaat ggcaaccctg aaactacagt tctggcgcat taccgaatgg
     6241 ctggaatttg cggaacggga acaaagccag atgatctgtt ggctgcatgg gcctgtagtg
     6301 actgccataa tgaaatcgat cgccgtactc gcattctcga caacaacgac gccagacttt
     6361 accacctgga aggcgtgatc aggacgctgg cgatattgct gaaggagggg aagattaagt
     6421 catgaatgaa tatgagtttg tgcttccctg gccgccgacg gtgaatacct actggcgaag
     6481 acggggaagc cagtactaca tcagcgataa aggccagaaa taccgaaaag acgtacagca
     6541 aatcatccgg caactcagat tagacatttt cactaaatca cgacttcgca tcacaattat
     6601 tgctgaacca ccagattccc gccgtcgcga cctcgataac atcctgaaag gtttactcga
     6661 ctctcttatc cacgccggat ttgcggaaga cgacgtgcaa ttcgatgaca ttcgcgtaat
     6721 tcgcggcgtg aaagtgcttg gcggtagagt ggggataaaa atcaccgaac tggagaacat
     6781 atgaacgcca caattcaaac aataccggaa ttgcttatcc aggcacgagg caatatgact
     6841 gaagtttcac gaaaactgaa ctgtaatcgt gctacggtca gaaaatatgt cggtgataaa
     6901 gaaggcaaac ggcacgccgt cgtcaacggt gtccttatgg ttcaccgtgg atgggataag
     6961 gggaaagcta ctgatgcgtg atattcagca ggtgcttgaa cgctggggtg catgggtggc
     7021 aaataatcat gaggatgtga gctggtcgcc cattgctgcc ggatttaagg ggctgatccc
     7081 cgaaaaagta aaatcacgtc cacagtgctg tgatgatgac gcgatgatta tatgcgggtg
     7141 tatggcccgc cttaacagaa acaacagtga tttgcatgag ttgctggttg attgctacgt
     7201 gcgggggatg acgtttatgt cactggcgcg aaaacatgaa tgctctgatg ggactatcgg
     7261 aaagcggctg caaaaagctg aaggtgttat tgaaggaatg ctgatgatgc tggatgttcg
     7321 gctggagatg gacagatatg tgcagcgcct gtgatagcag gcgcttgtcg ttcactatgt
     7381 gaagtatgtc aatttgtgaa cgttatgcta tttactttac aggacaagga cgtaagagtt
     7441 atctaccgtt taaccgcaga gatacaacaa caatgaacag ggggcaaaat ggacattgaa
     7501 gattttctgg aggcggaaga caatgatctg tctctgtgga gtgatgatat tttaagcatt
     7561 gactggttcg ttatcctcaa agagcttttg aaataccaga acaggttaaa gttaaactat
     7621 tcagaattac ttctgctggc taattttgtt tcatttcacc aggacgtaga ttcgcaaaca
     7681 ttaccatcaa tatctctttt tgccaccaga atgcgtacat cccgggacgc catacaagga
     7741 gtcctggtgt gtctggagga gaaagatatg ttacagaaga taagttacgc gcaaatcgat
     7801 catgacgatg atttgcgaaa catctatgat atcaagccat taatccgccg gttaatttgg
     7861 ttattgaaaa gtggttgtga taaaaagcat acttgccccc tctgcgggaa agtcgctata
     7921 tctaatgagg aaattgagaa aaaattcgga ttcagaattt gtgggaataa gaaaaggccg
     7981 caaagctggt gtcgttcatg cagaagcccc aaacaacgcc gactgaatac attgttatgt
     8041 ggtaaaaaaa gagctcgaaa atcctcgccc gaggtggtgt aaagcgtcgt agaaccccgg
     8101 tcctcttgtt tgattccata cctgaataaa gtgggatgcc aaaaaagtat tttacgtacg
     8161 taaaaatctg catatcatga taagagtggt tacattgcca cgcagccgaa cccgccgatg
     8221 cgcgggtttt tttgtaccca gaatcctgtg agctatacgg aaagtacaca gaaaggaagg
     8281 tgcgaccgca attaataaca aaatcttaaa aatatcatat ggcactatta gttttctaaa
     8341 tattgtatat tttaagtatt gcaggataac cctgtaacga agtttgcgta acagcatttt
     8401 gctctacgag tttgccagcc tcccctggtg gctggctttt tttgtatccc ttcaacatga
     8461 atgttacata cctcacaatt aagtcagttg aatgttgtct gcccggatga gaatttgtta
     8521 gaaaaaactg catggtgaat ccccctaagc ggcggggcat ctggcaaagt gtatgatcca
     8581 gcgaacatgc aaattcagta gccaggctga atttaccggg aggcacctgg caccatgcga
     8641 cagacagaaa ttaggctata cttcagcccc tctccggagg ggcttttctg tgcaggatgt
     8701 gtcacagttt cctgaattct gagtactgtc ctgttactca gggtgctata ttttctgacg
     8761 tgatgaaagt ctgccggaag gcggaacgta tcggaaatga cccagtagag aaaacgttga
     8821 ctcagatacc gatgctgagt taccgggaaa ccggcatcac atgaccgcta tccttccagg
     8881 ctcgctccgg cgggcctttt tactgcagaa aacagttttc ccgtaaaatg ccacgttact
     8941 cacaattcag gctggcgatt attgtctggc cagcgggaag tttgttaaaa aaatatggca
     9001 tggtgaatcc ccctgtgcgg aggggcatat cagcgacagg tgtttggtca caccccttat
     9061 cctttctggt gcgggttcag gtgctgatac tgaactcacc gggaggcacc cggcaccatg
     9121 catgaacggt acatagcgct actctccagc ccctctccgg aggggctttc ttgttggata
     9181 aaaaaaagcc cgcgccggga agcgcgggtg gcaaggaata aacaacaaaa cgtgaagtga
     9241 tcaattttca gctggcgaat aatacccgac agtaatcact ctgcgcaact gtatggtctt
     9301 tttcgtattg cgggctgtag tcatcctcct gtttctgaac ccttgtccat gactgacgtc
     9361 agcccgcacc ttatcttgat tgcatacact atccctgccg ggaggaataa tggcatttaa
     9421 acactatgac gtggtcaggg catcatcgcc gtcagacctc gcaggaaaac tgacgcaaaa
     9481 actgaaggaa ggctggcaac cctatggtag tccgattatc tcaaacgcgg gttatggtgc
     9541 tgaatttatc caggctatag tcgctgaagg ggatatgacc tctccggtag tttcaccggg
     9601 gggagatggt acccggactg ttgttgttga accagaatat tactttgtca ttgcgctggc
     9661 cggacagtca aacggcatgg cgtatggtga aggcctgccg ctgccggaga catatgaccg
     9721 tccggacccg cgtattaaac agctggcgcg tcgcagtacg gtgacaccgg gtggtgcggc
     9781 ctgtgcatat aacgacatca ttccggcgga ccactgcctg catgatgtgc aggatttgag
     9841 ccgtttttct catccgaaag ccagcgcaga gcagtatggg tgtgtcggac agggattgca
     9901 tattgccaaa aagctgttgc cctttatgcc ggaaaatgcg ggtattttgt tagtgccgtg
     9961 ctgtcgtggt ggctctgcgt ttacagccgg tgctgacgga acgttcagtg aaagttcagg
    10021 agcgtcggat aaatctgaac gatggggcgt tgataagccg ttgtataaag acctgctgac
    10081 acgcactcag gccgcgctga aggcaaaccc caaaaacata ctgctttgtg ttgtctggat
    10141 gcagggcgag tttgatttaa aacagggagc gtacgccagt cagcccgcca tgtttgatgc
    10201 catggtggaa aaataccgtt ctgacctggc gggagtatcc ggtcagtgcg ctggtgcttc
    10261 tccttctctg gtgccctgga tttgtggtga cacgacgtac tactggaagg agacgtattc
    10321 agcgcaatac gatgccgtct atggcgcata caaaaccaaa gccggtaaaa atatcttctt
    10381 tgtgccgttt atgatggatg acaatggtca gaaagtcggg accaatgaac cgtcagaaga
    10441 cccggatatt ccggctatcg ggtattatgg ttccggtgga cgaacggatg cgaagagctg
    10501 gacaacatcc gatcgtaaaa cccactttgg ttcatgggcg cgtcgcggaa ttatttccga
    10561 ccgtctggca acagccattc ttctgcatgt gggcagagtg gcagattttg tttccgggaa
    10621 agtgacaaaa gccgcggttt cttcaggcgg aggcgtatcg caaccagcac aggagagtgg
    10681 cgatactcag cctgaagccg ttgtcccgac aaagatacag tcagtgctgg cttatgatgt
    10741 gaatgcggaa aatgccgctc tggcttcgca gggctggacg ctgacagatg tcaaaaacac
    10801 gctggtcagt gaccgtggtt caggtaaaaa agcccttcgt cttgaaaaac cagaaaatgg
    10861 tcttgcacag aaaaagacct ggaaagtatc gcacgcaatt gatgccggta aggggaagga
    10921 gctgttcgat aacggcggtg aaatcacgct gcgctttaaa atccccgatg atgtatctct
    10981 caatgcttct gcaaatcaat attctgcggg tatttactgg cgcggtagtg gatggccggg
    11041 agcggaatca gaggagggtt atattgcggc cttctatctg cagactgata aaacaaacat
    11101 caatgtgatg tatcatgcaa acaccacatc acagcggctt ggtagttatg gtccgttcga
    11161 tcacgactgg cacacgctga ctttccgctt cccgggtggt ggctcgctga acgtcacgcc
    11221 ggtgcttgat aatgcagcag gaaaaccgtt tacgctgacc agatggacga atgcagcctt
    11281 tgaggccaac gcgctggcaa ttacggatat taccggaaat gcggcaactt accctgtgct
    11341 gattgaaagt cttactgttg gtgtgaatgc cgtagcagca taacagacga aaaaaaaccg
    11401 ccagcaacag gaatggagac tggcggaggt aatcccaatg gagaatctaa cgaaaggatg
    11461 ctttcgacat caatcatttc taaatgaaaa cagttctcat tgtcaacagt aacggtaata
    11521 aaccatgaca ttcatcaacc agttaatgct gtacttctgt acggtggtct gtgtgttgta
    11581 tcttctttca ggtggataca gggcagtacg cgacttctgg cgcagacaga ttgacaaaag
    11641 ggccgctgag aaaatcagcg ccactcagtc agccggagca aaaacagaag ccccactcat
    11701 tccggaacaa ccttcttaat aacccctttc aacgagaaaa tctcatgaca gatataaaac
    11761 aactggtcac tgctgaggca gtgaaggaag tcattcgctc tgaagaagtc agaagcgtcc
    11821 tgaaacaaaa actccgccag aatctggaag agcgtcttga tgcagaagtg gattcaattc
    11881 tggatgaatt gcttggtgca cagccggaac catccccgga actgcttccg gaaccacagg
    11941 cggaagatgt caccacggaa aatggtgata ttcagccgga gtcaccggtg acggatatga
    12001 cagacacaca acccgaaccg ggcacaatgc tgtaacggcg gggcagggcc atcagtaaga
    12061 cactgatggc cttttttatt tccggtagca caggtctgtc ggggcgggga tatgtatcag
    12121 atggaaaaaa tatcaacggg catagcctat ggtacttctg cgggcagtgc gggttactgg
    12181 tttctacagt ggttggatca ggttactccg tcacagtggg ctgctatcgg ggttttgggg
    12241 agcctgtgtc tggggttcct gacatacctg acgaa
//
LOCUS       DAZGJT010000121        12110 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZGJT010000121 DAZGJT010000000
VERSION     DAZGJT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.121"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..527
                     /locus_tag="ACHKFK_004036"
     CDS             105..527
                     /locus_tag="ACHKFK_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000049682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054264.1"
                     /translation="MSKYIYILLSFLVLFFIFFYAYISLMSKEHHYTQHELSPFFLYT
                     PESLRNLPNISNVAEYSYYYNVDDMQTRVIVTWRNIDNIFLQKAKLIDFLKRMGPSLQ
                     NDCIWFFHDKSDYANNFQRYCIIEHRDSLQVEYFETIE"
     gene            682..1446
                     /gene="yabI"
                     /locus_tag="ACHKFK_004037"
     CDS             682..1446
                     /gene="yabI"
                     /locus_tag="ACHKFK_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO9054265.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWYAWLAGIIGCLMGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLFWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(1560..2258)
                     /gene="thiQ"
                     /locus_tag="ACHKFK_004038"
     CDS             complement(1560..2258)
                     /gene="thiQ"
                     /locus_tag="ACHKFK_004038"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO9054266.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQKKMHAIAHQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSSSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGIKG"
     gene            complement(2242..3852)
                     /gene="thiP"
                     /locus_tag="ACHKFK_004039"
     CDS             complement(2242..3852)
                     /gene="thiP"
                     /locus_tag="ACHKFK_004039"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO9054267.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGDWV
                     SVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRQMLLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLWQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTALIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKIPGRNVKTD"
     gene            complement(3828..4811)
                     /gene="thiB"
                     /locus_tag="ACHKFK_004040"
     CDS             complement(3828..4811)
                     /gene="thiB"
                     /locus_tag="ACHKFK_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO9054268.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPVVKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEDKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     AEAVNVPGGWNNDTFVPYDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYEDPRT
                     STPGLGLLLWMQKVYGDNAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAATNFIEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     regulatory      complement(4842..4941)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(4975..6630)
                     /gene="sgrR"
                     /locus_tag="ACHKFK_004041"
     CDS             complement(4975..6630)
                     /gene="sgrR"
                     /locus_tag="ACHKFK_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO9054269.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQETTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     NIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQAGAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     gene            6719..6850
                     /gene="sgrT"
                     /locus_tag="ACHKFK_004042"
     CDS             6719..6850
                     /gene="sgrT"
                     /locus_tag="ACHKFK_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO9054270.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWEGSHSDY"
     gene            complement(6958..7563)
                     /gene="leuD"
                     /locus_tag="ACHKFK_004043"
     CDS             complement(6958..7563)
                     /gene="leuD"
                     /locus_tag="ACHKFK_004043"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO9054271.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEEKQPAFMR"
     gene            complement(7574..8974)
                     /gene="leuC"
                     /locus_tag="ACHKFK_004044"
     CDS             complement(7574..8974)
                     /gene="leuC"
                     /locus_tag="ACHKFK_004044"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO9054272.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            complement(8977..10068)
                     /gene="leuB"
                     /locus_tag="ACHKFK_004045"
     CDS             complement(8977..10068)
                     /gene="leuB"
                     /locus_tag="ACHKFK_004045"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO9054273.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPTATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELTHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            complement(10068..11639)
                     /gene="leuA"
                     /locus_tag="ACHKFK_004046"
     CDS             complement(10068..11639)
                     /gene="leuA"
                     /locus_tag="ACHKFK_004046"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO9054274.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            complement(11731..11817)
                     /gene="leuL"
                     /locus_tag="ACHKFK_004047"
     CDS             complement(11731..11817)
                     /gene="leuL"
                     /locus_tag="ACHKFK_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO9054275.1"
                     /translation="MTHIVRFIGLLLLNAYFLRGRRVSGIQH"
BASE COUNT         2724 a         3207 c         3218 g         2961 t
ORIGIN      
        1 gttcattatc aagttcggga gctataaagt tttcagaatc tccctttgaa agaccgcttt
       61 ttcttttctt aaaccctcat tatccgcaca ggtcgtgagt agagatgtca aaatatattt
      121 atattttatt atcattttta gttttattct ttatattttt ctatgcttat atctcattga
      181 tgtcaaagga acatcactat acacagcatg aactttcccc atttttcctt tatacgccag
      241 agagtttgcg aaatttgcca aacatatcaa acgtagccga gtatagctat tactataatg
      301 ttgatgatat gcaaacaaga gtgattgtta catggcgaaa tatagataac atttttcttc
      361 agaaagcaaa gctaatagat tttttgaaaa gaatgggacc gagtctacaa aacgattgta
      421 tatggttttt tcatgataaa agtgattatg caaataattt ccagcgttat tgcatcattg
      481 agcaccgtga ttctctgcag gtggaatatt ttgagactat cgaatgatag gcagagggtg
      541 tcttttgtgc cggttgtgaa gaaaaagtga atgatgtagc cgccaagttg tcataattgg
      601 taacgaatca gataattgac ggcttgacgg agtagcatag ggtttgcaga atccctgctt
      661 cgtccatttg acaggcacat tatgcaagca ttgctggaac actttattac ccaatccacc
      721 gtgtattcat tgatggcggt ggtgttggtg gcctttctgg agtcgctggc gctggtcggt
      781 ttgattctgc ccggtacggt gctgatggcg gggctgggag cgctgattgg tagcggtgag
      841 ttaagtttct ggtacgcctg gctggcgggg attattggct gcctgatggg cgactggatc
      901 tccttctggc tgggctggcg ttttaaaaag ccgttgcatc gctggtcatt tctgaagaag
      961 aacaaagcat tactcgataa aaccgaacat gcgctgcatc agcacagcat gttcaccatt
     1021 ctggtcggtc gttttgtcgg cccgacgcgt ccgctggtgc caatggtggc gggaatgctg
     1081 gatctcccgg tggcaaaatt tattacgccg aatattatcg gctgcttgct gtggccaccg
     1141 ttttacttcc tgccggggat tctggcgggt gcggcgatcg atattcctgc cgggatgcag
     1201 agcggtgagt ttaaatggtt actgctggca acagcggtgt ttttgtgggt tggtggctgg
     1261 ctgttctggc ggttatggcg cagcggtaaa gcgactgacc gtttgagtca ttatttgtcc
     1321 cgcggtcgtt tgttgtggct gacgccgttg atttctgcca tcggcgtggt ggcgctggtg
     1381 gtgttaattc gccacccgtt gatgccggta tatatcgata ttttgcggaa agtggttggg
     1441 gtttagggga tagccttgtg cgggttgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     1501 tgcaaaacgc actacccgta ggtcggataa ggcgttcacg ccgcatccga cagtgcatat
     1561 taacctttaa ttcccaacag cgccgaagca ctcgccttac cgctcaacaa ctcatcggtc
     1621 ttaccctgcc aggcgatacg cccgtcggcg accaccaccg agcgcgtggc gatccgcgcc
     1681 gcatcttcca cactgtgcga caccatcaat agcgtcattt tttgctgttg gcagctcgaa
     1741 ctcaccagcg tcagcatctc ctgacgtagc gccggatcga gcgcggaaaa cggttcatcg
     1801 agcaataaaa tgggctgttc gcgtaccaga caacgcgcta acgccacgcg ctgtcgttga
     1861 ccgccggaaa gctcgccagg taaccgcgcc attaaattat caatccccat ctggtgggca
     1921 atcgcgtgca ttttcttctg ctgcgccgca tttagtttca accccggatt tagccccagc
     1981 ccgatgttct gcgcgaccgt caggtggctg aacaggttgt tctcctgaaa cagcatcgac
     2041 accggacggc gtgacggcgg cgtcgtggtg tgatctacgc cgtcgatagt cagcgaaccg
     2101 ctggctggcg tcagaaaacc ggcgatcaaa ttaagcaggg tacttttacc cgcgccgctt
     2161 ggcccgagga tcgccacctg ctcgccgcgt tccaccgtta agctaaaacg catcggcaaa
     2221 tggtggtaaa gccaggtgat atcagtcagt tttaacattt cgccccggta ttttttcaat
     2281 cacggtaaac agcagaaaac agagcagcag cagaattaac gcggtgaccg cgccgtcctg
     2341 gctgcgatag gagccaattt gctggtagag ataaaacggc agggtacgga aatcatcgtt
     2401 accgaacaat gccaccacgc caaaatcacc aatcgacagc acacaagcaa aggccagcgc
     2461 ctgcgccagt ggacgtttca gggcgcgcag ctccaccact tttaagcgtg accagccttc
     2521 aatgcccagc gactgacaca acatgctgta gcgggcggtg atatcgcgca tcgggttttc
     2581 cagtactttc agcgcataag ggattgccat taacgcattg gtgaaaatca caatgccgtc
     2641 agcagattgc ggcaggccga tagtgttgtt gagcagtaaa aagaagcccg tagccagcac
     2701 aatccccggc atggcgagga tcaacatgcc gctcatctcc agcgcctgac ccgccagcat
     2761 tttctgccgc gcccgaagtt cgcgactgct ccatagcagc atcatggtca gcactacgca
     2821 caatacgcct gccgccagcg caatacgcaa cgaggtccac agcgcctgcc acagcaccgg
     2881 ttgtgccagc acttccggca actggcgatt taccccatcg acgatcaccg ccagtaacgg
     2941 tggcagcaac agcagcagcg ccagcacaat taacgccgtg tcgcaaatgc gactatgcag
     3001 acgatcgtcc gggtcgcgcc agccttgcag cagtgtggta ccaggcgcaa tggccttact
     3061 caatcgctga ctcaacagca ccagcccgag gcagcacacc atctggatca gcgccagcat
     3121 tgccgcgcgg gcaggatcgt agtcgtaact cagtgcctga tagattgcca gctcgatagt
     3181 ggtcgcctgc ggaccgccgc ccagcgatag cacggtggcg aagctggcga aacagagcat
     3241 aaagattagt gcggcaaccg gcgggatttg tcgccgtaac cacggccatt cgacgaaacg
     3301 gaaaaaatgc cagccgcgca tcccaagttg ggcggcaagc tgacgttgtt cgccgggaat
     3361 gttttccagt gcctggagta ataaacggct agccatcggc agattaaaaa acacatgggc
     3421 cagcaaaatg ccttgcaggc cgtagggcga aaaggtccac tccagaccaa gtgattgcca
     3481 gagcgaggcc agccagccct ggcgaccata gacgctaaga atgccgaaaa cggcaaccag
     3541 caccgggagg atcaaggtca ttgcgcacag acgcaacagc atctgtcgcc caggaaagcg
     3601 cctgcgatag agggcgcggg cgaggaatat tgccggtacg acagagagca gcgccgagag
     3661 aaacgcctgc cagaaggaga agcgcaccac atgccacagg tagctgtcct gccagactga
     3721 cacccagtca ccctgcggcg cgttccacca cagggcgaga aacgccgcca gtgcaaccgc
     3781 taccaccagc gtggcggcgc ttacgcctgg aattaaccag ccgggaatta acggctgacg
     3841 gcgcgttgcc attcgctaat ccatgcctga cgttgtgccg ccacttcggc gggtgtgaac
     3901 tctagtgtgg tggctggttt ggtcaattgt tcaaatcctg caggcagagt gacgtttgct
     3961 accggataca tccagttgcc ggttgggatc gcattctgaa aagccggaga aaccataaac
     4021 tggaggaatt tttgcgccag ctctggctgc ttgctggcag cggtgcgggc ggcaacttcc
     4081 acctgcagat agtgaccttc gataaagttt gtggcggcgt agttatcttt cttctcttcg
     4141 agaatgtgat aagccggaga ggtggtgtaa ctcagtacca gatcgctttc gccttttaaa
     4201 aacaggccgt aggcttcgct ccagcctttg gtgaccgtga cagttttctt cgccagtttc
     4261 tgccaggctt gcggggcgtt atcgccatag actttttgca tccatagcaa cagacccagc
     4321 cccggtgtac tggtacgtgg atcttcataa atcacccgcc agttttgatc gctctcaacc
     4381 agttctttca ggctttgcgg cggatttttc agtttgttct tgtcatagac gaaggcgaaa
     4441 tagccgtaat catacggtac gaaagtgtca ttattccagc cgccaggaac gttaacggct
     4501 tccgctgcca caccgctttt agcaaacagc cctgttttgc tggcggcgtc taacagattg
     4561 ttatccagcc ccagcaccac atcggctttg ctgtttttgt cttccatccg tagacggttg
     4621 agcagcgaaa cgccatcttc cagcgccacc agtttcagtt cgcaattaca gtctgcttca
     4681 aaggcttttt tgactaccgg accaggtccc cagtcagcgg caaaggaatc gtaggtataa
     4741 acagttagaa caggtttagc gaaaacaggc gctgtgcaca gcaacagcag gggaagacat
     4801 ttttttaaca ctttgcacct cacaaaagag tggcaaagga cttgagaagg agcctcaaat
     4861 cccttcgccg gcgttatccg gatcaggttc gacgggtatt ttctcagcgc acgcggctgc
     4921 gtggcacccc gttgagaacg gcgttagtgt agtgattttg ttatcaacca gcaatcatgg
     4981 atccggtggt gcaaaccacg ctgatttaaa atcgaaccag ccgagggtgt tcatgcgcag
     5041 gccgcgcata ctacgttgcc cctgaatgat cagccagtgg tggataagtg gcaccattgc
     5101 tttgctggcg accagttgct ggcaccagtt cgccaggttc atttcgccat tgcgccagcg
     5161 ggcggcgccg gcttgccagt cgatcggaat acagtgttgg agcagcggca cctcgcacaa
     5221 gtgcgcgaac agcgaaaaat ccagcggcag cgtaaagttg gcgctgttaa gccagatatc
     5281 gctctcgatc tctccttcat gccactgatc gtagctgatc tctttgattt ccagcgtgac
     5341 ctggtgactt gccagaatct gctgcatgat cccggcaatc acccgatgct cgctgtgatc
     5401 ctgataaaag gtcagggtga ggctttccag accagccggt ttttcgctct ttatggtgcg
     5461 ggcatggtgc caacggggga gcagtccata agccgggaac cacagttgct ggtactgttc
     5521 ctcagcgaaa tagaccagat tcgtcggaga aagcacatag cttacccagt ctctgacttg
     5581 ctgattcgcc cctcgatggg tgcggctgtc gaacagtaaa tagtagcaac cttcctccag
     5641 gcggctttca atctcttttt cctcgccctg tggacctttt agcatcagcc ctccggctgg
     5701 ctcgtcggca atttccggca gaacccagac gttaacttcg tcgattaatg cccggtaacc
     5761 gaagaagtca tcgaatgcct gaattttcag ttgattggtg ctgttgcgga tcaccgcata
     5821 cggaccggtg ccgatgggat ggctggcaaa gttactgagg gtttcccatt cgcgcggcag
     5881 gatcattgcc ggaacttgcc ccagcagtaa cggcaaccag cggtccggtt gcgtgagatg
     5941 gatatccagc gtccagggcg tcggcgacac aatgttagca atatgcgaat agagcggaag
     6001 cgtattgatt cgttttaaag aggcgatcac atcgtccatt tccagttcac gaccatggtg
     6061 aaaatggact cctggacgca aaaagaaacg ccagtgaagc ggtgatattt gctgccagtg
     6121 gtgtgcgatg tctgcttcca gttccccatt ttcctcattt atgcgcgtta gcgaactgaa
     6181 gatttgccgg gcgatatggg tttcagaacg gcgcaatgcg ctgccaggta gcagattacg
     6241 caacggacga tagtagagca cgcgcaggat gtgccgcccc tggcggaagc tgcggcccag
     6301 atgagaaacc agcatttgcc gcacagtcgc tttgtcgcca accaactgca ctagttggtc
     6361 gatacgatcc tgctccagca ggtcttccgc ccgctgttgc tgaagcgcca gcccggtata
     6421 gaggaatgtc aggcgtgaac gtttaccgcg cccgacttcc gcttcccacg tcagccagcc
     6481 gcgatcctgc atggtgttga gcagggtgcg catatgacga cgcgagcaac tcagtaacgc
     6541 tgccagttcg ttgagcgtgg tctcctgaga tttaccctcg cagcattgcc acaggcggat
     6601 gaactgttgt tgcagacgag cagatggcat aaaaggggaa ctcctgcgca aaagacagca
     6661 attttatttt ccctatatta agtcaataat tcctaacgat gaagcaagga ggtgccccat
     6721 gcgtcagttt tatcagcact attttaccgc gacagcgaag ttgtgctggt tgcgttggtt
     6781 aagcgtccca caacgattaa ccatgcttga agagctgatg cagtgggagg ggagtcattc
     6841 tgactactga tttgcggaca tcatgtgtga ctgagtattg gtgttaatcg ccacgccagc
     6901 agtgattatc tgctggcttt tttctttttg gcgattgagt gccggacggt gtacaaacta
     6961 acgcataaac gccggttgct tctcttcata agacgcaata gcatcgtcgt gctgcagagt
     7021 caggccgatg ctgtccagac cgttcatcat gcagtggcgg cggaaggcat cgatggtaaa
     7081 gcgataagtt ttctctcccg ctttcacctc ttgcgcttcc aggtctacat caaaatgaat
     7141 ccccggattg gctttcacca gcgcaaacag ctcatccact tctgcatcgc ttaatttcac
     7201 cggcagcagc tggttgttaa agctattgcc gtagaagatg tcagcaaaac tcggcgcaat
     7261 caccacctta aaaccgtaat cggtcaatgc ccacggcgcg tgctcacgcg aggaaccgca
     7321 gccgaagttt tctcgtgcca gcaaaatgga agcgccctga tactgcggga agttcagcac
     7381 gaagtccggg tttggctgtt ggcctttttc atccagaaaa cgccagtcgt taaacagatg
     7441 cgcgccaaaa cccgtacggg tcactttctg caaaaactgt ttcgggatga ttgcatcggt
     7501 atcgacattg gcggcatcca acggaaccac caggcctgtg tgtttgataa atttctctgc
     7561 catggtgtgc tccttattta atgttgcgaa tgtcggcgaa atgtccggtc acagccgcgg
     7621 cggcagccat tgccggactg accagatgcg tgcgcccgcc gcgcccctgg cggccttcaa
     7681 agttacggtt gctggtggag gcgcaacgtt cgcccggatt caggcggtcg ttgttcatcg
     7741 ccaggcacat tgaacaacct ggcaagcgcc attcaaaacc agcttcgata aaaattttgt
     7801 ccagaccttc cgcttccgcc tgggctttaa ccggaccgga gccgggaacc accagcgctt
     7861 gtacgcctgg cgcgactttc cggcctttgg cgatttccgc tgccgcgcgt aaatcttcaa
     7921 tacgcgagtt agtacaggaa ccaataaaca ctttgtcgat agccacttct gtcagcggaa
     7981 tacccggttt cagccccata taggccagcg ctttttctgc cgacgcgcgt tcaaccggat
     8041 cggcaaacga agccggatcg ggaatattgt cgttcacaga aatcacctgg cctggattgg
     8101 tgccccaggt gacctgcggc gaaatctctt ctgcttgcag agtgacaacg gtatcgaaag
     8161 ttgctccttc gtcggtttgc agggttttcc agtaggcaac ggcgtcgtcg aaatctttgc
     8221 ctttcggcgc atgcagacgg cctttaacat agttaaaggt ggtttcgtcc ggtgcaacca
     8281 gaccggcttt tgcgcccatt tcgattgcca tattgcacag ggtcatacga ccttccatgc
     8341 ttaaatcacg gattgcttcg ccgcaaaatt ccaccacatg cccggtaccg cctgcgctgc
     8401 cggttttacc aataattgcc agcacgatat cttttgcggt aatgcccggc gcggctttgc
     8461 cctggacttc aattttcatg gtcttcgccc ggccctgttt cagggtttgc gttgccagta
     8521 cgtgttcaac ttcggaagtg ccgataccaa aggccagtgc gccaaacgcg ccgtgggtgg
     8581 cggtatgtga gtcgccgcag acaatggtca tccccggcaa ggtcacgccc tgttccggcc
     8641 ccattacgtg gacgatcccc tgatacgggt gattcaggtc atacagctcg acgccaaatt
     8701 ctttgcagtt tttgatcagc tcctgcatct ggatgcgcgc catttcaccg caggcattaa
     8761 tgtctttcgt ctgagtagag acgttgtgat ccatggtggc gaaggttttg cccggctgac
     8821 gtaccgggcg accgtgggcg cgcagaccat cgaacgcctg cggtgaggtc acttcatgca
     8881 ccaggtggcg gtcgatatat aacagcgggg tttcgttttc ggcttcgtac actacgtgag
     8941 cgtcgaataa tttttcgtat aacgtcttag ccatgattac accccttctg ccacatagcg
     9001 ggcaatgata tcgcccattt catcggtact aacggcggca gcgccacggg ctaaatcccc
     9061 agtgcgaatg ccttcttcta atgcgcggtt aatggcgcgt tcaatggcgg aagccgcatc
     9121 atcggcatcc aggctgtaac gcagcagcag cgccagcgac agaatttgtg caatcgggtt
     9181 ggcgatattt ttacctgcga tatcgggtgc cgagccgccc gccggttcat acagaccaaa
     9241 accttgctcg ttcaggctgg cggaaggcaa catccccatt gagccagtga tcattgcgca
     9301 ctcatcagac agaatgtcgc caaacaggtt ggagcacagc aagacgtcaa actgtgatgg
     9361 atctttaatc agctgcatgg tggcgttgtc gatgtacata tgcgtcagtt cgacgtccgg
     9421 gtattccgtg gcgatctcgt taacgatctc ccgccataaa atagaggatt gcagcacgtt
     9481 ggctttgtcg attgaggtga ctttgtggcg acgcttgcgg gcagattcaa atgcgatgcg
     9541 ggcaatacgc tcgatctcaa aacggtggta cacctcggta tcaaacgctt tttcatattg
     9601 tccgctacct tcgcggcctt ttggctgacc gaaatagatg ccgccggtca gttcgcgcac
     9661 acacaggatg tcaaagccgt ttgcggcaat gtcagcacgc agtgggcaga acgcttccag
     9721 cccctgatac agttttgccg gacgcagatt gctgaataat ttgaagtgtt tacgcaaagg
     9781 cagcagtgcg ccgcgttctg gttgctggtc tggcggcaaa tgttcccact tcggaccacc
     9841 taccgagcca aacagcacgg catcggcttg ctcacaacct tcaaccgtcg cagtcggcag
     9901 cggctgtccg tggttatcaa tggctgcgcc gcctacatcg taatggctgg tggtgatacg
     9961 catcgcaaat cggttgcgca cggcatccag cactttcagc gcctgggtca tcacttccgg
    10021 accaataccg tcccccggca atacggcaat atggtaattc ttcgacatca cacggtttcc
    10081 ttgttgtttt cgttgtgttg agctttgcgt tgcaactctt tttcgacttc tgcggcacgc
    10141 cagatattgt tcagtacgtg caccatggct ttggcggagg actcgacaat atcggtggcc
    10201 aggccgacgc cgtggaagcg gcgaccgttg tagttggcga caatatccac ctgacccagc
    10261 gcatctttac cgtggccttt ggcggtcagg ctgtatttca ccagttcgac gttatagtca
    10321 gtgatgcggt ttatcgcctg gtagacggca tcgaccggac cgttaccgtt ggcggcttct
    10381 gctttgactt cttcgccaca ggccagtttg acggcggcag tggcaatatc gttagaacct
    10441 gactgcacgc tgaagtaatc cagacggaaa tgctccggct cttcttgctg cttaccgatg
    10501 aaggccagcg cctccagatc gtaatcaaac acctggcctt ttttgtccgc cagcttcagg
    10561 aaagcgtcgt acaggttgtc taaattatat tcactttctt tatatcccat ctcatccatg
    10621 cgatgtttca ccgccgcacg ccccgaacga gaggtcagat tcagctggat ttggtttaga
    10681 ccaatagatt ctggtgtcat gatttcgtag ttttcgcggt ttttcagtac accatcctgg
    10741 tggataccgg aggagtgtgc gaatgcgccg ctgccaacaa tggctttgtt tgccgggatc
    10801 ggcatattac aaatctggct aactaactgg ctggtgcgcc atatctcctg gtgattgatg
    10861 gcggtgtgga cgttgagaat atccttacga actttgatcg ccatgatgac ttcttcaagg
    10921 gaacagttac cggcacgctc gccgatcccg ttcattgcgc cttctacctg ccgtgctccg
    10981 gcatgtaccg ccgccagtga gttgccgacc gccaggccca aatcgtcgtg ggtatgtacg
    11041 gaaataatgg ctttgtcgat gttgggtacg cgttcataca ggccgctgat gattccggcg
    11101 aactcaaatg gcatggtgta gcccacggtg tccggaatgt tgatggtggt ggcaccggca
    11161 ttaatcgccg cttcgaccac tcgcgccaga tcggcaattg gggtgcgtcc ggcatcttcg
    11221 caagaaaatt caacatcatc ggtgtaatta cgggcgcgtt tcaccatata gatagcgcgt
    11281 tcgatcactt cgtccagcgt gctgcgcagc ttggtggcga tgtgcattgg cgaagtggca
    11341 ataaaagtat ggatacggaa ggcttcggcg actttcaggg attcggctgc cacgtcaata
    11401 tctttttcca cgcagcgagc taacgcgcat acacggctgt ttttaacctg gcgagcgatg
    11461 gtctgcactg attcaaaatc acccggcgaa gagacgggga aaccgacttc catcacgtca
    11521 acacccatac gctcaagggc cagcgcaatt tgcagttttt ctttcacact caagcttgcc
    11581 tgtaacgcct gttcaccgtc gcgcaatgtg gtatcgaaaa taatgacttg ctggctcatg
    11641 gtttgggtcc ttgtctcttt ttgagcgcct cgcttcgggc ataaaaaaac ccgcgcaatg
    11701 gcgcgggttt ttgtttgact gcgtgctggc ttaatgctgg atgccgctca ctcgtctacc
    11761 gcgcaaaaaa tatgcgttta gtagtagtag accgataaag cgaacgatgt gagtcattaa
    11821 atcagctcca gatgaatgcg atatgctttt agagttactg gatacaaaaa cggatgtcaa
    11881 ccctgaagca ataaaaacgt cacgccattg tgagttcggc atccgtaaaa ttagctaatt
    11941 gtgctgcggt ggttaaaaca agcgatatta atttctgctt aaccgccgat gcttttcatc
    12001 ggttgacata tttcagaata attttttgca tctaatcaac gagggaaagg gggataaaat
    12061 gcacgcgtta caaaacctat cctgatgatt tgtattgaat tatatgtttt
//
LOCUS       DAZGJT010000122        11970 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZGJT010000122 DAZGJT010000000
VERSION     DAZGJT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.122"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..258)
                     /locus_tag="ACHKFK_004048"
     CDS             complement(<1..258)
                     /locus_tag="ACHKFK_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9054276.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVH
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCG"
     gene            415..1344
                     /gene="metA"
                     /locus_tag="ACHKFK_004049"
     CDS             415..1344
                     /gene="metA"
                     /locus_tag="ACHKFK_004049"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO9054277.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            1613..3214
                     /gene="aceB"
                     /locus_tag="ACHKFK_004050"
     CDS             1613..3214
                     /gene="aceB"
                     /locus_tag="ACHKFK_004050"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO9054278.1"
                     /translation="MTEQATTIDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYSNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGETIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLDKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSHGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            3244..4548
                     /gene="aceA"
                     /locus_tag="ACHKFK_004051"
     CDS             3244..4548
                     /gene="aceA"
                     /locus_tag="ACHKFK_004051"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO9054279.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            4764..6488
                     /gene="aceK"
                     /locus_tag="ACHKFK_004052"
     CDS             4764..6488
                     /gene="aceK"
                     /locus_tag="ACHKFK_004052"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO9054280.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKGHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIVRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDRFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPLPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRFV"
     gene            complement(6505..7329)
                     /gene="iclR"
                     /locus_tag="ACHKFK_004053"
     CDS             complement(6505..7329)
                     /gene="iclR"
                     /locus_tag="ACHKFK_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO9054281.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGIR"
     gene            7529..11212
                     /gene="metH"
                     /locus_tag="ACHKFK_004054"
     CDS             7529..11212
                     /gene="metH"
                     /locus_tag="ACHKFK_004054"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO9054282.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPAEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPTELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANTQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKMLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAE"
     gene            11272..11592
                     /locus_tag="ACHKFK_004055"
     CDS             11272..11592
                     /locus_tag="ACHKFK_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054283.1"
                     /translation="MIKIDEIDFFAFEGCSESEVTELLGKKPTGYNDWIINIKTGVEW
                     VFYDGRYWLDFRKSKEGIRCISCSILYHDKSKGRIDVLFSEGIADRGLKNTGGVLIQK
                     RPQL"
     gene            11635..>11970
                     /locus_tag="ACHKFK_004056"
     CDS             11635..>11970
                     /locus_tag="ACHKFK_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054284.1"
                     /translation="MQRQYKKYILLFWGVMDVIAIATYLFYSLGNGRIPFYSDIVHSL
                     SLLKDIGVEGGFYAYAVATVTLQIVLLFSLFFSAQCFLRQKLFSFSFLAIQEFMRFAT
                     VSCSISVIPL"
BASE COUNT         2899 a         3019 c         3206 g         2846 t
ORIGIN      
        1 gccgcagccg cgcacatcag gatcgataaa cagcgcatcc atatgctgcc cacttaacaa
       61 cataaaccca accggctgat cccgctcatt aaccgcgacc cacaacggtg cttccggcag
      121 aaaggaatgc accagctctt ccagctcggc ccgatactct gctgataaaa aatcgtgagt
      181 ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc ccctcctcat gccgtgagcg
      241 tcgaatacta ataaccattt tctctccttt tcgtcattct tatattctaa cgtagccttt
      301 tccttgaaac tttctcacct tcaacatgca ggctcgacat tggtaaattt tctggttatc
      361 ttcagctatc tggatgtcta aacgtataag cgtatgtagt gaggtaatca ggttatgccg
      421 attcgtgtgc cggacgaact acccgccgtc aatttcttgc gtgaagaaaa cgtctttgtg
      481 atgacaactt ctcgcgcgtc tggtcaggaa attcgtccgc taaaggtgct tatccttaac
      541 ctgatgccga agaagatcga aactgaaaat cagtttctgc gcctgctttc aaactcacct
      601 ttgcaggtcg atattcagct gttgcgcatc gattcccgtg aatcgcgcaa cacgcccgca
      661 gagcatctga ataacttcta ctgtaacttt gaagatattc aggaacaaaa ctttgacggt
      721 ctgatagtta ccggcgcgcc gctgggcctg gtggagttta atgatgtcgc ttactggccg
      781 cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca cctcgacgct gtttgtctgc
      841 tgggctgtac aggccgcgct caatatcctc tatggtattc ctaagcaaac ccgcaccgac
      901 aaactctctg gcgtttacga gcatcatatt ctccatcctc atgcgcttct gacgcgtggc
      961 tttgatgatt cattcctggc accgcattcg cgctatgctg actttccggc agcgttgatt
     1021 cgtgattata ccgatctgga aattctggca gagacggaag aaggggatgc atatctgttt
     1081 gccagcaaag ataagcgcat tgcctttgtg acgggccatc ccgaatatga tgcgcaaacg
     1141 ctggcgcagg aatatttccg cgatgtggaa gccgggctag acccggatgt accgtataac
     1201 tatttcccgc acaatgatcc gcaaaataca ccgcgagcga gctggcgtag tcacggtaat
     1261 ttgctgttta ccaactggct caactattac gtctaccaga tcacgccata tgatctacgg
     1321 cacatgaatc caacgctgga ttaatcttct gtgatagtcg atcgttaagc gattcagcac
     1381 cttacctcag gcaccttcgg gtgccttttt tatttccgaa acacacctca gtaagtgaat
     1441 aaattttatt tatattgtta tcaataggtt aacatgtatt tttaattaaa atggaaattg
     1501 tttttgattt tgcattttaa atgagtagtc ttagttgtgc tgaacgaaaa gcgcacaacg
     1561 atccttcgtt cacagtgggg aagttttcgg atccatgacg aggagctgca cgatgactga
     1621 acaggcaaca acaatcgatg aactggcttt tacaaggccg tatggcgagc aggagaagca
     1681 aattcttact gccgaagcgg tagaatttct gactgagctg gtgacgcatt ttacgccaca
     1741 acgcaataaa cttctggcag cgcgcattca gcagcagcag gatatcgata acggaacgtt
     1801 gcctgatttc atttcggaaa cagcttccat tcgtgatgct gactggaaaa ttcgcgggat
     1861 tcctgcggac ttagaagatc gtcgcgtaga gattaccgga cctgtagagc gcaagatggt
     1921 gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc gatttcgaag attcactggc
     1981 accagactgg aacaaagtga tcgatgggca aattaacctg cgcgatgcgg ttaacggcac
     2041 catcagctat agcaatgaag caggcaaaat ttatcagctc aagcccaatc cagcggtttt
     2101 gatttgtcgg gtacgcggtc tgcacttgcc ggaaaaacat gtcacctggc gtggtgagac
     2161 aatccccggt agcctgtttg attttgcgct ctatttcttc cacaactatc aggcactgtt
     2221 ggcaaagggc agcggtccct atttctatct gccgaaaacc cagtcctggc aggaagcggc
     2281 ctggtggagc gaagtcttca gctatgcaga agatcgcttt aatctgccgc gcggcaccat
     2341 caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc cagatggatg aaatccttca
     2401 cgcgctgcgt gaccatattg ttggtctgaa ctgcggtcgc tgggattaca tcttcagtta
     2461 tatcaaaaca ttgaaaaact atcccgatcg cgtcctgcca gacagacagg cagtgacgat
     2521 ggataaaccc ttcctgaatg cttactcacg cctgttgatt aaaacctgcc ataaacgcgg
     2581 tgcttttgcg atgggcggca tggcggcgtt tattccgagc aaagatgaag agcgcaataa
     2641 ccaggtgctc gacaaagtaa aagcggataa atcgctggaa gccaataacg gtcacgatgg
     2701 cacatggatc gctcacccgg gccttgctga tacggcaatg gcggtattca acgatattct
     2761 cggctcccgc aaaaaccagc ttgaggtgat gcgcgaacaa gacgcgccga ttactgccga
     2821 tcagctgctg gcaccttgtg atggtgaacg caccgaagaa ggtatgcgcg ccaacattcg
     2881 cgtggctgtg cagtacatcg aagcgtggat ctctggcaat ggctgtgtgc cgatttatgg
     2941 cctgatggaa gatgcggcga cggctgaaat ttcccgtacc tcaatctggc agtggatcca
     3001 tcatcaaaaa acgttgagca atggcaaacc ggtgactaaa gccttgttcc gccagatgct
     3061 gggcgaagag atgaaagtca ttgccagcga actgggcgaa gaacgtttct cccatgggcg
     3121 ttttgacgat gccgcacgct tgatggaaca gatcaccact tccgatgagt taattgattt
     3181 cctgaccctg ccaggctacc gcctgttagc gtaaaccacc acataactat ggagcatctg
     3241 cacatgaaaa cccgtacaca acaaattgaa gaattacaga aagagtggac tcaaccgcgt
     3301 tgggaaggca ttactcgccc gtacagcgcg gaagatgtgg tgaaattacg cggttcagtc
     3361 aatccggaat gcaccctggc gcaactgggc gcggcgaaaa tgtggcgtct gctgcacggc
     3421 gagtcgaaaa aaggctacat caacagcctc ggtgcactga ctggtggtca ggcgctgcaa
     3481 caggcgaaag cgggtattga agcagtctat ctgtcgggat ggcaggtagc ggcggacgct
     3541 aacctggcgg ccagcatgta tccggatcag tcgctctatc cggcaaactc ggtgccagcg
     3601 gtggtggagc ggatcaacaa tactttccgc cgtgcggatc agatccaatg gtccgcgggc
     3661 attgagccgg gcgatccgcg ctatgtcgat tacttcctgc cgatcgtcgc cgatgcggag
     3721 gccggttttg gcggtgtact gaatgccttt gaactgatga aagcgatgat tgaagccggt
     3781 gcagcggcag ttcacttcga agatcagctg gcatcagtga agaaatgtgg tcacatgggc
     3841 ggcaaagttt tggtgccaac tcaggaagct attcagaaac tggtcgcggc gcgtctggca
     3901 gcagatgtga cgggcgttcc aaccctgctg gttgctcgta ccgatgccga tgcggcggat
     3961 ctgattacct ccgattgcga cccatatgac agcgaattta ttactggcga gcgtaccagt
     4021 gaaggcttct tccgtactca tgcgggtatt gagcaagcga tcagccgtgg cctggcgtat
     4081 gcgccatatg ctgacctggt atggtgtgaa acctccacgc cggatctgga actggcgcgt
     4141 cgctttgcac aagccatcca cgcgaaatat ccgggcaaac tgctggctta taactgctcg
     4201 ccttcgttca actggcagaa aaacctcgac gacaaaacta ttgccagctt ccagcagcag
     4261 ctgtcggata tgggctacaa gttccagttc atcaccctgg caggtatcca cagcatgtgg
     4321 ttcaacatgt ttgacctggc aaacgcctat gcccagggtg agggtatgaa gcactatgtt
     4381 gagaaagtgc agcagccgga atttgccgcc gcgaaagatg gctatacctt cgtatctcac
     4441 cagcaggaag tgggtacagg ttacttcgat aaagtgacga ccattattca gggcggcacg
     4501 tcgtcggtta ccgcgctgac cggctccacc gaagaatcgc agttctaagc aaccgataaa
     4561 cgttgctgac tgtaggccgg ataaggcgtt cacgccgcat ccggcaatca gcgcctgatg
     4621 cgacgcttgc gcgtcttatc atgcctacag ccgttgccga acgtaggccg gataaggcgt
     4681 ctacgccgca tccggcaatc agcgcctgat gtgacgctta cgcgtcttat ccggcaattc
     4741 tctgctcccg acgagggcaa caaatgccgc gtggcctgga attactgatt gcacaaacca
     4801 ttttgcaagg tttcgacgct cagtatggtc gattcctcga agtgacttcc ggggcgcagc
     4861 agcgtttcga gcaagccgac tggcatgctg tccagcaggc gatgaaaaac cgtattcatc
     4921 tttacgatca tcacgtgggt ctggtcgtgg agcaactgcg ctgcattact aacggccaaa
     4981 gcacggacgc ggcattttta ctgcgcgtta aagggcatta cacccggctg ttgccagatt
     5041 acccgcgctt cgagattgcg gagagctttt tcaactccgt gtactgtcgg ttatttgacc
     5101 accgctcgct tactcccgag cggcttttta tttttagctc tcagccagag cgccgctttc
     5161 gtaccattcc acgcccactg gcgaaagact ttcaccccga tcacggctgg gaatctctgc
     5221 tgatgcgcgt tatcagcgat ctgccgctgc gtctgcgttg gcagaataaa agccgtgaca
     5281 ttcattacat cgttcgccat ctgacggaaa cgctggggac agacaacctc gccgaaagtc
     5341 atttacaggt ggcgaacgaa ctgttttacc gcaataaggc agcctggctg gtgggcaaac
     5401 tgatcacgcc ttccggcacg ttgccatttt tattgccgat ccaccagacg gacgacggcg
     5461 agttatttat tgatacctgc ctgacgacga ccgccgaagc gagcattgtt tttggctttg
     5521 cgcgttctta ttttatggtt tatgcaccgc tgcccgcagc gctggtcgag tggctacggg
     5581 aaattctgcc aggtaaaacc accgctgaat tgtatatggc tatcggctgc cagaagcacg
     5641 ccaaaaccga aagctaccgc gaatatctcg tttatctaca gggctgtaat gagcagttca
     5701 ttgaagcgcc gggtattcgt ggaatggtga tgttggtgtt tacgttgccg ggttttgatc
     5761 gggtatttaa agtcatcaaa gacaggttcg cgccgcagaa agagatgtct gccgctcacg
     5821 ttcgtgcctg ctatcaactg gtgaaagagc acgatcgcgt gggccgaatg gcggacaccc
     5881 aggagtttga aaactttgtg ctggagaagc ggcatatttc cccggcatta atggaattac
     5941 tgctccagga agcagcggaa aaaatcaccg atctcggcga acagattgtg attcgccatc
     6001 tttatattga gcggcggatg gtgccgctca atatctggct ggaacaagtg gaaggtcagc
     6061 agctgcgcga cgctattgaa gagtacggca acgccattcg ccagcttgcc gctgccaaca
     6121 tcttccctgg ggatatgtta tttaaaaact ttggcgtcac ccgtcacggg cgcgtggtgt
     6181 tttatgatta cgacgaaatt tgctacatga cggaagtgaa tttccgcgac atcccgctgc
     6241 cgcgctaccc ggaagacgaa cttgccagcg aaccgtggta cagcgtctcg ccgggcgatg
     6301 ttttcccgga agagttccgt cactggttat gtgccgaccc gcgtattggt ccgctatttg
     6361 aagagatgca cgccgacttg ttccgcgctg attactggcg cgcactacaa aaccgcatac
     6421 gtgaagggca tgtggaagat gtttatgcgt atcggcgcag gcagcggttt agcgtccgat
     6481 tcgtataaat cagaggcgaa aaagtcagcg tattccaccg tacgccagcg tcacttcctt
     6541 cgccgcttta atcaccatcg cgccaaactc ggtcacgcgg tcatcggtaa tacgtgaaat
     6601 cggtccggaa attgaaattg cggcaaacgg ttcgcggtgc tcatcgaaaa tacaggctgc
     6661 aaggcaacgt agccccagtg catgttcctc atcgtcaaat gaataacccc gtttgcgcgt
     6721 ttgggcgaga tcttctttta aatgcacagg agacaccagc gttgcgtggg tataggcatg
     6781 taaccctttg cggtgcagca gcttcgtcac ctgttcttcg ctcagttggg ccagaaaggc
     6841 tttacccgca ccggaagcgt gcatcggcaa tttaccgccg ataggcgcgg acattcgcat
     6901 caggtgagta cactgtacct ggtcgataat aatcgcttcg tgatcgcttt gatcaagcac
     6961 cgccatattg accgtttcgc cagactcttc catcagattg cgcagaatag gatggacaat
     7021 cgctaacaaa ttacggctct ggagaaagct gctgccgacc ataaaggcgt gtgcgccgat
     7081 cgcccagtga cccagttcgc cgacctgacg gacaaagccc tgctgttgca tcgtggttag
     7141 caggcggtgg gtcgtggaat tgggtaaccc ggcttgttgc gccagttccg tgagtgccac
     7201 actgccattg gattcggcaa tccactccag taatttcagg ccacgcgtta aagactgaac
     7261 ctgtccagtc gctggtgcgg tggcaacggc gggttttctg ccgcgttttg cgggaatggg
     7321 tgcgaccatg acagtctcct ttttctgtat cgtggaaatc attttcattt ttattatcag
     7381 cgaatgcaat agttgctgaa cagatccgat gagttaatgt tgaacaaatc tcatgttgcg
     7441 tggtggtcgc ttttaccaca gatgcgttta tgccagtatg gtttgttgaa tttttattaa
     7501 atctgggtta agcgtgtcgg gagcaagtgt gagcagcaaa gtggaacaac tgcgtgcgca
     7561 gttaaatgaa cgtattctgg tgctggacgg cggtatgggc accatgatcc agagttatcg
     7621 actgaacgag gccgattttc gcggcgagcg ttttgccgac tggccgtgcg atctcaaagg
     7681 caacaacgac ctgctggtgc tcagcaaacc ggaagtgatc gccgctatcc acaacgccta
     7741 ctttgaagcg ggtgcggata tcatcgaaac caacaccttc aactccacga ccattgcgat
     7801 ggcggattac cagatggagt ccctgtcggc ggaaatcaac tttgcagcgg cgaaactggc
     7861 gcgcgcctgt gccgacgagt ggactgcccg cacgccggag aaaccgcgct acgttgccgg
     7921 tgttctcggc ccgaccaacc gcacggcgtc tatttctccg gacgtcaacg atccggcatt
     7981 ccgcaacatc acttttgacc agctggtggc ggcttatcga gaatccacca aagcgctggt
     8041 ggaaggtggc gcggacctga tcctgattga aaccgttttc gacaccctta acgccaaagc
     8101 ggcggtcttt gcggtgaaaa cggagtttga agcgctgggc gttgaactgc cgattatgat
     8161 ctccggcacc atcaccgacg cctccgggcg cacgctctcc gggcagacta ctgaagcgtt
     8221 ttacaactcc ctgcgccacg ccgaagcgct gacctttggc ctgaactgtg cgctggggcc
     8281 agacgaactg cgccagtacg tacaggaact gtcgcgtatt gcggaatgct acgtcaccgc
     8341 ccacccgaac gccgggctgc ccaacgcctt tggtgaatac gatctcgacg ccgacacgat
     8401 ggcaaaacag atacgtgaat gggcagaagc aggctttctc aatatcgtcg gcggctgctg
     8461 tggcaccacg ccacaacata ttgcagcgat gagtcgtgca gtagaaggat tagcgccgcg
     8521 caaactaccg gaaattcccg tagcctgccg tttgtccggc ctggagccgc tgaacattgg
     8581 cgaagatagc ctgtttgtga acgtgggtga acgcaccaac gtcaccggtt ccgctaagtt
     8641 caagcgactg atcaaagaag agaaatacag cgaggcgctg gatgtggcgc gtcagcaggt
     8701 ggaaaacggt gcgcagatta tcgatatcaa catggatgaa gggatgctcg acgccgaagc
     8761 ggcgatggtg cgttttctca atctgattgc cggtgaaccg gatatcgctc gcgtgccgat
     8821 tatgatcgac tcctcaaaat gggacgttat tgaaaaaggt ctgaagtgta tccagggcaa
     8881 aggcattgtt aactctatct cgatgaaaga gggtgtcgac gcctttatcc atcacgcgaa
     8941 actgttgcgt cgctacggtg cggcagtcgt ggtaatggct tttgacgaac agggccaggc
     9001 cgatacccgc gcacggaaaa tcgagatttg ccgtcgggcg tacaaaatcc tcaccgaaga
     9061 ggttggcttc ccggctgaag atatcatctt cgacccgaac atcttcgcgg tcgcaactgg
     9121 cattgaagag cacaacaatt acgcgcagga ctttatcggc gcgtgtgaag acatcaaacg
     9181 tgaactgccg cacgcactga tttccggcgg cgtttctaac gtttctttct cattccgtgg
     9241 caacgatccg gtgcgcgaag ccattcacgc ggtgttcctc tactacgcta ttcgcaatgg
     9301 catggatatg gggatcgtca acgccgggca actggcgatt tacgacgacc tgcctaccga
     9361 gctacgcgac gcggtggagg atgtgatcct caaccgtcgc gacgatggca ccgagcgttt
     9421 actcgagctt gccgagaaat atcgcggcag caaaaccgac gacaccgcta acacccagca
     9481 ggcggagtgg cgctcgtggg aagtgaataa acgtctggaa tactcgctgg tcaaaggcat
     9541 taccgagttt atcgagcagg ataccgaaga agcccgccag caggctacgc gtccgattga
     9601 agtgattgaa ggcccgttga tggacggcat gaacgtggtc ggcgacctgt ttggcgaggg
     9661 caaaatgttc ctgccgcagg tggtgaaatc ggcgcgcgta atgaagcagg cggtggcgta
     9721 tctcgaaccg tttattgaag ccagcaaaga gcagggtaaa accaacggca agatggtgat
     9781 cgccaccgtg aaaggcgatg tccacgacat cggtaaaaat atcgttggcg tggtgctgca
     9841 atgtaacaac tacgaaattg tcgatctcgg cgtgatggtg cctgcggaaa aaattctccg
     9901 taccgctaaa gaagtgaatg ctgatttgat tggcctttca gggctgatca cgccgtcgct
     9961 ggacgagatg gttaacgtgg cgaaagagat ggagcgtcag ggcttcacca ttccgctatt
    10021 gattggcggc gcgaccacct caaaagcgca cacggcggtg aaaatcgagc agaactacag
    10081 cggcccgacg gtgtatgtgc agaacgcatc gcgcaccgtt ggtgtggtgg cggcgctgct
    10141 ttccgacact cagcgcgatg atttcgttgc tcgtacccgc aaggagtacg aaaccgtgcg
    10201 tattcagcac ggacgaaaga aaccacgcac accaccggtc acgctggaag cggcgcgtga
    10261 taacgacttc gcttttgact ggcaggctta cacgccgccg gtggcgcacc gtctcggtgt
    10321 gcaggaagtc gaagccagca tcgaaacgct gcgtaattac atcgactgga cgccgttctt
    10381 tatgacctgg tcgctggccg ggaagtatcc gcgcattctg gaagatgaag tggtaggtgt
    10441 tgaggcgcag cggctgttta aagacgccaa cgacatgctg gataaattaa gcgccgaaaa
    10501 aatgctgaac ccgcgtggcg tggtgggcct gttcccggca aaccgtgtgg gtgatgacat
    10561 tgaaatctac cgtgacgaaa cgcgtaccca tgtgatcaac gtcagccacc atctacgcca
    10621 acagaccgaa aaaaccggct tcgctaacta ctgtctcgct gacttcgttg cgccgaagct
    10681 ttctggtaaa gcagattaca tcggcgcatt tgccgtgact ggcgggctgg aagaggacgc
    10741 actggctgat gcctttgaag cacagcatga tgattacaac aaaatcatgg tgaaagcgct
    10801 tgccgaccgt ctggcggaag cctttgcgga gtatctccat gagcgtgtgc gtaaagtcta
    10861 ctggggctat gcgccgaacg agaacctcag caacgaagag ctgatccgcg aaaactacca
    10921 gggcatccgt ccggcaccgg gctaccctgc ctgcccggaa catacggaaa aagccacaat
    10981 ctgggagctg ctggaagtgg aaaaacatac cggtatgaaa ctcacggaat ccttcgccat
    11041 gtggccgggt gcatcggttt cgggttggta cttcagtcat ccagacagca agtattacgc
    11101 ggtggcgcaa attcagcgcg atcaggttga agactatgcc cgccgtaaag gaatgagcgt
    11161 taccgaagtt gagcgttggt tggcaccgaa tctggggtat gatgccgagt aagtgataac
    11221 accggggtat ttgccccggt ttttaatgtc ataaataatg gattattgaa catgataaaa
    11281 attgatgaaa tagatttttt tgctttcgaa ggatgttcag agtccgaagt tactgaacta
    11341 ttaggaaaaa aaccaacagg ttataatgat tggataatta atataaaaac aggtgtggag
    11401 tgggtatttt atgatggtcg ttattggtta gattttcgta aatccaaaga gggtataagg
    11461 tgtatctcat gcagtattct gtatcacgat aaaagtaaag ggcgtatcga tgtcttgttt
    11521 agtgaaggta tagctgacag aggtttaaaa aatacgggtg gagtgttaat ccaaaaacgc
    11581 ccgcaattat aataattcct ggtaatattt gttttcgtaa aaaggattta ctttatgcaa
    11641 cgtcaatata aaaaatacat tcttcttttt tggggtgtta tggatgttat cgcgatagcc
    11701 acatatctgt tctactcgct tggtaatggt cgtatccctt tttattccga tatcgttcat
    11761 tcactttctt tacttaagga tatcggtgtt gaaggcggtt tttacgctta tgcagtcgcc
    11821 actgttacgc tacaaatagt attattgttc tctttattct tttctgcgca atgtttttta
    11881 agacaaaaat tatttagttt ctcttttttg gcgattcagg agtttatgcg ttttgctacc
    11941 gtaagctgtt caatctccgt aataccgttg
//
LOCUS       DAZGJT010000123        11617 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZGJT010000123 DAZGJT010000000
VERSION     DAZGJT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.123"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..855
                     /locus_tag="ACHKFK_004057"
                     /pseudo
     CDS             1..855
                     /locus_tag="ACHKFK_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1198..2496
                     /gene="kgtP"
                     /locus_tag="ACHKFK_004058"
     CDS             1198..2496
                     /gene="kgtP"
                     /locus_tag="ACHKFK_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO9054285.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLIEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAVLREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAASLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLATIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLTLHRKGKGMRL"
     gene            complement(2493..2816)
                     /gene="yfiM"
                     /locus_tag="ACHKFK_004059"
     CDS             complement(2493..2816)
                     /gene="yfiM"
                     /locus_tag="ACHKFK_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO9054286.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDFAWDIAGASTGYTVW
                     QLTRH"
     gene            complement(2862..4217)
                     /gene="pssA"
                     /locus_tag="ACHKFK_004060"
     CDS             complement(2862..4217)
                     /gene="pssA"
                     /locus_tag="ACHKFK_004060"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO9054287.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(4331..6991)
                     /gene="pat"
                     /locus_tag="ACHKFK_004061"
     CDS             complement(4331..6991)
                     /gene="pat"
                     /locus_tag="ACHKFK_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO9054288.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHKDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWNAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     VISNPLDLRDDASCEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESALALIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKICARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLKLNLAQREES"
     gene            complement(7023..7721)
                     /gene="tapT"
                     /locus_tag="ACHKFK_004062"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(7023..7721)
                     /gene="tapT"
                     /locus_tag="ACHKFK_004062"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO9054289.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSLDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(7790..8209)
                     /gene="trxC"
                     /locus_tag="ACHKFK_004063"
     CDS             complement(7790..8209)
                     /gene="trxC"
                     /locus_tag="ACHKFK_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO9054290.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            8416..9453
                     /gene="yfiF"
                     /locus_tag="ACHKFK_004064"
     CDS             8416..9453
                     /gene="yfiF"
                     /locus_tag="ACHKFK_004064"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO9054291.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEESPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(9501..10190)
                     /gene="ung"
                     /locus_tag="ACHKFK_004065"
     CDS             complement(9501..10190)
                     /gene="ung"
                     /locus_tag="ACHKFK_004065"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO9054292.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIATPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGEKPI
                     DWMPVLPAESE"
     gene            10495..10878
                     /gene="grcA"
                     /locus_tag="ACHKFK_004066"
     CDS             10495..10878
                     /gene="grcA"
                     /locus_tag="ACHKFK_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO9054293.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGFAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10933..11520)
                     /gene="eamB"
                     /locus_tag="ACHKFK_004067"
     CDS             complement(10933..11520)
                     /gene="eamB"
                     /locus_tag="ACHKFK_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO9054294.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATTHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQT
                     KPISFWASFALQFVNVKIILYGITALSTFVLPQTQALSWVIGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2893 a         2905 c         3026 g         2793 t
ORIGIN      
        1 aagggcacga tgagctggtg agttggctga aatgtcacaa gattgaccat gcgcatatct
       61 gcatcgaagc gaccggcacc tatatggaac ctgtcgcaga gtgcctttac gatgctggct
      121 acatagtgtc agtcattaat cctgcactgg gtaaagcttt cgctcagagt gaaggactgc
      181 gtaacaagac tgataccgtg gatgccgcat gctggcagag ttctgtcgtc agaagcgccc
      241 tgcagcctgg gaagcgcatc acccgcttga acgcgcgttg catgccctgg tagtacgcca
      301 ccaggcgctg acagatatgc acaggcagga actgaatcgc actgaaacgg cgcgggaagt
      361 ccagaggccg agcattgatg ctcaccttct gtggcttgaa gcagagctga agcgtcttga
      421 gaagcagata aaagacctga cagacgatga tccggatatg aaacaccgca ggaaactgct
      481 ggaaagcatc ccgggtatcg gagagaaaac atctgcggta ttgctggctt acatggtctg
      541 aaggaccgtt ttgcccatgc cagacagttc gccgcttttg cgggtctgac accacggcgt
      601 tatgaatcag gcagtagtgt gagaggggca agccggttga gtaaagccgg acatgtgtcg
      661 cttcgcaggc cgttgtatat gcctgcaatg gtagccacca gtaagactga gtggggacgg
      721 gcgttggcag ctaatggcaa gaaaggaaag gtgattctcg gctcgataat gcgcaagctg
      781 gcacaggtgg cgtatggagt gctgaagtca ggcgtgccgt tcgatgcgtc acggcataat
      841 ccggtagctg cgtaaaaatc gcggaaggga tgaaacaaca gcgcctgacg gcgctgtgtc
      901 tggcatgcct gcaatccggg agactggacc aggaaaaaac ttgctggccg taacagtatc
      961 tacgctccca cataagccag attcaacagc gaatacgtct tccccaaatt gcccacttcc
     1021 atacttcctc cttaccagaa atctatcctt aagctcctta ataaccattt tcctgctaac
     1081 taaattcatg gttaaggttg cataatgata cgcaacaaat gtataacatt tttcttacca
     1141 aaaaataaac aaaagcgacc gacaaaagca tcggattacg gcaggagaca taatggcatg
     1201 gctgaaagta ctgtaacggc agacagcaaa ctgacaagta gtgatactcg tcgccgtatt
     1261 tgggcaattg ttggggcctc ttcaggtaat ctgatcgagt ggttcgattt ctatgtctac
     1321 tcgttctgtt cactctactt cgcccacatc ttcttccctt ccgggaacac gacgactcaa
     1381 ctactgcaaa cagcaggtgt ttttgctgcg ggatttctga tgcgcccaat aggcggttgg
     1441 ctatttggcc gcatagccga taaacatggt cgcaaaaaat cgatgctatt atcggtgtgt
     1501 atgatgtgtt tcggctcact ggttattgcc tgtttgccag gctacgaaac aatcggtacc
     1561 tgggctccgg cattattgct tcttgctcgt ttattccagg gattatccgt tggcggagaa
     1621 tatggcacca gcgccaccta tatgagtgaa gttgccgttg aagggcgcaa aggtttttac
     1681 gcatcatttc agtatgtgac gttgatcggc gggcaactgc tagctctact ggttgtcgtg
     1741 gttttacaac acaccatgga agacgctgta ctcagagagt ggggatggcg tattcctttc
     1801 gcgttagggg ctgtgttagc tgttgtggcg ttgtggttac gtcgtcagtt agatgaaact
     1861 tcacaacaag aaacacgcgc tttaaaagaa gctggatctc tgaaaggatt atggcgcaat
     1921 cgccgtgcat tcatcatggt tctcggtttt accgctgcgg cctccctttg tttctatacc
     1981 ttcaccactt atatgcagaa gtatctggta aatactgcgg gaatgcatgc caacgtggcg
     2041 agtggcatta tgactgccgc attgtttgta ttcatgctta ttcaaccact tattggcgcg
     2101 ctgtcggata agattggtcg ccgtacctca atgttatgtt tcggttctct ggcaaccatt
     2161 tttaccgttc ctattctctc agcattgcag aacgtttcct cgccttatgc cgcttttggt
     2221 ctggtgatgt gcgccctgct gatagtgagt ttttatactt caatcagtgg aatactgaag
     2281 gctgagatgt tcccggcaca ggttcgcgca ttaggcgttg gtctgtcata tgcggtcgcc
     2341 aatgctatat ttggtggttc ggcggagtac gtagcgttgt cgctgaaatc aataggaatg
     2401 gaaacagcct tcttctggta tgtgaccttg atggccgtgg tggcgtttct ggtttcacta
     2461 acattgcacc gtaaaggaaa agggatgcgg ctttagtgac gggtcagttg ccagacggta
     2521 tagccggtgc ttgcaccggc gatatcccag gcaaaatcct tccagctcca gccgctcccc
     2581 tcagggcggc tatcccaaag ctcttttgac gcccccagac tgacagagaa catcaatcca
     2641 aacatggcac tgcgatcccg gctcatcccc tgatgctgtg aatattcatt tccggcggcg
     2701 gaaagcatcg ccgaggcgat aaagtgttga gctttatcct gcccgctcca gctatcgtta
     2761 gccatatggc tgcatccaga aagtaacaat agcgaacaga caaaaagaat acgcaaaacg
     2821 tggcctccaa aaaacaaacc ccgtacagga cggggttgtg attacaggat gcggctaatt
     2881 aatcggtcga tgcggatacg gcgcaaacgg cggatgagtt tacgaacctt caccggataa
     2941 tcggcaatac tttgcagatc gcgatagtgc ttaacgatgg tggtatgctc gcggatcagc
     3001 tccagttctt tctctcgctg cggcgcaagc tccagttgtg gatcgtggat caaaatggcg
     3061 ttttccagat ccagacgcca ggcgcgcggg ttcaggttat taccggtgat caacatccac
     3121 ttatcatcaa cccacatccc ttttaggtga taggtgttgt cgtcatcttt ccataaccga
     3181 accactagct ggtcagtatt gacgtaatac tgcaaacggc tcaggaaacg acgcagattg
     3241 atctcataga gataaggcaa tgcgccaatt atcttgaaag gttcatcttc cggaatgtag
     3301 aagtcattcg cggttttatc accaacaata atttcgacct ttttcccttc gcgcagcaac
     3361 tggataatat tgcgcacaag gattgctggc aggttgaagt atggcgtaca gatggttagt
     3421 ttctgctcgg cacaaggcat aagatggaaa atggtcttgt tcaacagact cgatttcccc
     3481 agccccacta gcggcgttac ggaaagctga tcgttgtcgg catcgccctg gaaatgataa
     3541 gcggcatcac gcagctcctg gcggaacaga cgaatatcgt tcttgatttc cgggcttttt
     3601 ggccgattaa catcatccag acgattaacg ccgcggccat tcataatatt ctgtgtaacc
     3661 cattcaaaca taatgtctga catcttacgg ttacggataa gatgataacg gtcgtagcga
     3721 tatttatcgt gctgatgcag gtaaacatcg ttcaggctgg caccgctata aagtacgcta
     3781 tcgtcgatga taaagccttt aaagtgcaga acaccaaggg cttcacgggt attgattggc
     3841 acgccataaa ccggaacatc tacgcccgga ttttcctgcg ccatgcggca gtaccagtca
     3901 gcgttagtgt tagatgccgc agcgccaatg cgtccacgtt gtgcacgatg ccagtcgacc
     3961 agcacccgca catccagttc cggacgctgc cttttagcct catacaacgc gttcagaatg
     4021 cctttgccac cgtcatcctg ttcgagatac agggcgacaa tgcaaatgcg ctgcttcgcg
     4081 ctggctattt tttccagcag cgtctcccgg aagtcggcgg gagcgtaaaa gaaatcgaca
     4141 tcatcaactg attgagaaat cttgggtagt tgggcaaggt gttgttgatg tttattacgc
     4201 ttaaattttg acaacatcac agtgcatttc ttctctgttc attgaagggt cctctgtgca
     4261 atgcagacga cataagcggg caataataac accagtcccg attaagtggt caacatttcc
     4321 agtaccttac tcatgattcc tcgcgctggg caagatttag cttaagccca acgatcccct
     4381 cttcgagctg gatatcaacg ttaaacccga gcttgcgggc cagcgccacc atgccacgat
     4441 tgtttggcat cgtaatacca ttcagacgtt gcagtccgtg atctcgcgta taggtaatca
     4501 atttttccat taagcgtcga cctaagccta accctttgag atccgagcga accagcacgg
     4561 caaattcggc atcgatgtta tcagggtcgg agatcgcacg cgtgacgccg aggatctctt
     4621 ccgtttgatc aatacgtcgt accgctacaa atgccatttc ccgatcgtag tcgatctgcg
     4681 tcatgttggc taaatcttca tgggtaaatt cgttgatctc gctaaagtag cggtaataaa
     4741 gatcttcttt ggtgactcgc gaaatgaact gctgaagttg tggctcatct tctggcaaaa
     4801 tcgggcggaa caagcagcgt tcaccgtttt tcaattctac ccactcttcc agctgatgtg
     4861 gataagggcg cactgccagc cggctctcgt tatcgccttc aaacggcgcg atatccagcg
     4921 tgacatccag cgcggtaaat tcactgccag aagccagcaa aggatgaata tccagccgtt
     4981 gaatttccgg gcaatcgaca atcaagttgg aaacctgcac cagaagctgg ctcaagcctg
     5041 caacatccaa tggacgcagt gcactgcgcg cacaaatctt tttacttttt attccctgaa
     5101 tgaccagata acgggccagg ttcatgttca gcggcggtaa tgcgacgacg gcttgatctt
     5161 caggacgcca ctccacaccg ccttcaccca gcatgatcaa cggcccgaaa accggatcgt
     5221 gctcaaccac aacccgcaac tcctgagcgc cagcacggtt agccatactt tgcactaaca
     5281 ggccgtggac ccgcgcctgc ggccaggcca tttttacgcg atcgaaaata gcgtttgccg
     5341 cttgctggac ttcattggct gtacgtaggt agagcatgac gccctgaact tccgatttat
     5401 gtggaatatc cggcgagcgc aatttcagtg ccaccggata gccaatctgt tcggcaatgt
     5461 gtaccgcttc ggtgctatca ctggcaatcc aggtagggag agtgttcata ccatacgctt
     5521 gcaggatggg ctgaacttca tgggtatcga gcgacgtagc cccttcggca atcgcctgtt
     5581 gcaacagaag atgcgcttct gcggtattgg aagtcagatt gctgggcagt gccggcgttt
     5641 cgcgtagttg cttctggtta cgtcggtact ccaccatatg cataaaagcg gtgatggttc
     5701 cttccggggt acggtaggtc ggcagcccgg cttcgctgaa taaacgtcgc gcctcctgcg
     5761 aagagtgttc gccgcaccag ttcgtcagca gagagacata cttgctgcga gggtgatgct
     5821 ttaccgcttc aattaatgcc agtgcgcttt ctgtggcggg agcagcagca ctgggcgaat
     5881 gaataaccat cagcgcatca aaatcctggc tgtggagtag aatgtccagc gttttgacat
     5941 aatgctcgca actagcgtca tcgcgcagat cgagcgggtt agagattact acatgttctg
     6001 gcagcgcatc gcgtagtttc tggcaggttt cttcacttag cgttgccagc ttgccattgc
     6061 gtgaccataa agcatccagc gccagcgcag ctggcgctgc accgttgctg ataatcatca
     6121 gtcggtcgcc acgcaatggg cgcatatggc taagggtttc tactgccgaa aacagctcgt
     6181 gggtatcctg cactcgcaac aaaccggcgc gctgaatagc tgcattccag gcaggatcca
     6241 ttcctgccgt ggtgttcagt agtcgttgcg ccgccgggct acgtccgctt ttaatcacca
     6301 gaatcggttt attacgtgag gcgctacggg ccgccgaaac aaaacgtcgc gcatcactta
     6361 attgttcgag atagagcagg atggcgctgg ttttactgtc gcgcgccaaa tagtcgagca
     6421 attcatcaac gtcgatatcc aggctgtcgc cgagcgcaat aaagtaggaa aagcccatct
     6481 cacgctgttg cgcccagtcg aggatggtgt tggagacagc agccgattgc gaaataaacg
     6541 ccagcttgcc gcgtttaatc ggcacaggtg aaaagctggc atttagacct tgccagggag
     6601 ccagtaagcc cagactgttt ggtcctagca ggcgcatgtt atggcgcagg gcgcaggcgc
     6661 ggagatcttt gtgttgcgat gccggggcgg aaagaataat gcaggtttta cagcctttct
     6721 cgccgagctc ttccagaaga gcaagattac ggctggcatt ggtacataaa accgcaaggt
     6781 cgggtgtaaa gggcaagctg gcaatatccg gccaggccaa cacacccaac accgctttcc
     6841 aggctggcgt caccgggagt accggtccgt taaagcctcc cgccagcagg ttacgcatca
     6901 tcaggtaacc tgcgcgattg ggtttcatcg atgcgccaat taccgcaatc gattttggtc
     6961 gcaatagtgc ttccagtcct cgctgactca taccggtctc ccgcagaagt gaccgaatga
     7021 ttttaaacgc tttctaactg ttctgctgtg atgctaccca gatgttgcgt ttttcctgcc
     7081 agatagcgtg ttttaaagcg ggtaaaatgc tcgcctaacc ctgccgccgc cccggtatcg
     7141 ccggccatat ctaacagtgc gatggctacc tcggcagtac aatattggcc ttcagcctgg
     7201 gcttcacgca ggcgataggc agaaagtcgg gaaagatcga cggaaatgac gggaagatta
     7261 tccagatacg gacttttacg aaacatcttg cgagcttcag gccaggtacc atcgagcatg
     7321 ataaatagcg gaggcttacc ggcaggtggt gtgaagatca cttcccgttg ctcatcagca
     7381 tacgaggcgg gaaagaccac cattggctga tagtccgggt tttgtaccag atccagcaaa
     7441 tctagcgagg gttcggtacg cgaccattga aacgcaacgg tatcaggcaa aatatcagca
     7501 atcagacgcc cggtattact gggcttcatt ggctcggtgt cgaacatcag caaacagaag
     7561 cgactttttg cttgtgctgg ggtaattgtc gaacagagac ataatttctc tggtaaaagg
     7621 cagcgttggc agcgacgaac gcgattaccg cgggcaagaa aaggacgtgt tgcgcgcgca
     7681 atacgctcgg cgcgtaactg gagaacagcg ttttcggtca taagagagcg tcgaaaaaac
     7741 gccattgtcg cagaggagaa aacggggcac aagatgcgcc ccggtaagat taaagagatt
     7801 cgttcagcca gctatcaaac ggcgctttcg gtactgcgcc attaagcatg tcgacaacct
     7861 gaccgttttt gaaaatcatg atcgtcggta tactacgaat tccaaagcga ctgcttaatt
     7921 cacgttcagc ttcggtattc actttcacaa agcgcacttt accgctacgc tcttgcgcga
     7981 catcttcaaa aattggtgcg aaattacggc aggggccgca ccacggtgcc cagaagtcga
     8041 tcaccacagg tagatcatct ttcagcaatt tgtcgagcgt ttcaccggtc gcattaatca
     8101 cctctccgtc aaacaagtcg tgaccgcagc gtccgcattt tgccgcatct tcgatccgat
     8161 cgtcgggaat gcgattgatg gcctgacaat gggtacaaac ggtattcata actaacctcg
     8221 ggatgagtaa ggagacttat cggcaacgac gccggtatgt ttctaatatg ttacatatta
     8281 tcggggacat tgttccagac aacaatctgt tttattcaat aggtatgata gtcgcaggct
     8341 ttggtacgaa atcatggcga accgcgcgga cttcgggtaa tctgcgcgct tcgcgcagcg
     8401 ctggtggaga aaagcatgaa cgatgaaatg aaaggtaaaa gcggcaaggt caaagtgatg
     8461 tatgtccgca gtgatgatga ttctgataaa cgtacccaca acccgcgtac cgggaaaggg
     8521 ggcgggcgtc caggaaaatc tcgtgctgac ggtggccgtc gccccgcccg cgatgacaaa
     8581 cagagtcagc cccgtgaccg caagtgggaa gagtcgccgt ggcgcacagt ttcccgcgcg
     8641 ccgggtgatg agacgccgga aaaggccgat cacggtggca tcagtggtaa aagttttatt
     8701 gatccggaag ttttgcgtcg tcagcgtgcg gaagaaaccc gcgtctacgg cgaaaatgcc
     8761 tgtcaggcac tgttccagag ccgtccggaa gcgattgttc gcgcctggtt tatccagagt
     8821 gtaacgccgc gttttaaaga agccttgcgc tggatggcag caaaccgcaa agcgtaccat
     8881 gtggtagatg aagcggaatt gacaaaagcg tcaggcacgg aacatcacgg cggcgtttgc
     8941 ttcctgatca aaaagcgtaa cggtacaacc gtgcagcagt gggtaagcca ggcaggcgcg
     9001 caggattgtg tactggcgct ggaaaacgaa tctaacccgc ataacctggg tggcatgatg
     9061 cgcagctgcg cgcacttcgg cgtgaaaggc gttgtggtgc aggatgcggc actgctggag
     9121 tcgggggcgg ctatccgtac cgcagaaggc ggcgcagagc acgttcagcc gattactggc
     9181 gacaacattg ttaacgtgtt ggatgatttc cgtcaggcag gttacaccgt agtgaccact
     9241 tccagcgagc aggggaaacc gctgttcaaa accagtctgc cagcgaaaat ggtactggtg
     9301 ctgggtcagg aatatgaagg gttaccggat gccgcacgcg atccgaacga tttgcgcgtg
     9361 aagattgatg gtactggcaa cgttgccggg ctaaatatct ctgttgcaac cggcgttttg
     9421 cttggtgaat ggtggcgtca gaataaagcc tgataaatca gccgggtggc aacgctgcca
     9481 tccggcgttt tcctgcaaat ttactcactt tctgccggta atactggcat ccagtcaatc
     9541 ggtttctcgc cacgttgttc cagccactga tttgccagca caaaatggtt gcagccaaag
     9601 aacccacgat gcgccgaaag cggtgatgga tgcggcgctt tcagtacgtg atggcgttgt
     9661 ttatcaataa tcgccccttt cttctgtgca tgtgatcccc acaacaaaaa caccacgcct
     9721 tcgcggtgct ggttaatcag gctgatgact ttatcggtga aggtctccca gccgaggctg
     9781 gcgtgagaat gcgcctgacc tgcgcgaacc gtcaacacag tattgagtag cagaacgccc
     9841 tgacgcgccc agctttcaag ataaccatga ttagggcggg tgaaaccggg aatagtattt
     9901 tccagctctt tatacatatt cagcagtgac ggcggtgtcg caatgccggg gcgaacggaa
     9961 aatgccagac catgcgcctg tcccggtccg tgataaggat cctggccgag aatcaccact
    10021 ttaacgtcac ccaactctgt aaagcggaac gcgttaaaga catccttttg tggcggatag
    10081 atagtgacgc cggactgccg ctcgctggcg acggtctgta gggtattaag aaaatagggt
    10141 tgttgcttct cttcagccag cacgtcatgc caggttaatt cgtttgccat ctcgctctcc
    10201 cgcgaatctt caatccgcct agcttaactg cttctttcta cagaacaaaa tcatgctcat
    10261 tttttagact taaacgagtg caaaaaaatg taaattggta aaatttcaca cctgcataag
    10321 ttgatgtaaa acaataaaat ccatttgcag ccatacttga tgtgtggttt ttattgattt
    10381 aaatcaaaga ttcaagggtg tttgaggagt atatatacac tcaagcaaca atggttttac
    10441 caattggccg cgacaggctg aacaaatcaa ataattttgc cggggaggca tcacatgatt
    10501 acaggtatcc agattactaa agccgctaac gacgatctgc tgaactcttt ctggctgctg
    10561 gacagcgaaa aaggcgaagc gcgttgcatc gttgcgaaag caggttttgc agaagacgaa
    10621 gtggttgcag taagcaaact gggtgacatt gagtaccgtg aagttccagt agaagtgaaa
    10681 ccagaagttc gcgttgaagg tggtcaacac ctgaacgtaa acgttctgcg tcgtgaaact
    10741 ctggaagatg cagttaagca tccggaaaaa tatccgcagc tgaccatccg tgtatccggt
    10801 tatgcagttc gctttaactc tctgactccg gaacagcagc gcgacgttat cgctcgtacc
    10861 tttactgaaa gcctgtaagg cttaagtagc aagttactaa gcggaagagg gcgacctctt
    10921 ccgctttttt cattaataga aaatgcgtac cgcgcaatag accagcagca gcgcgagcac
    10981 gatatttaac tggcgaccat actggcgaaa caatcgctga aacaggtgcc ccgccagcgc
    11041 ccagcacaca ttgccaaacg tcccaatcat cgccagcaaa acgctgacgc caattaccca
    11101 gcttagcgcc tgcgtttgcg gcaggacaaa cgtcgacagt gctgtaatgc catacaaaat
    11161 gattttgacg ttcacaaact gcaaagcaaa gctggcccaa aaactgattg gttttgtctg
    11221 aagcccatct tcctttgttg gactggtagc gattttccac gccagccaga caatatatgc
    11281 cgcccccgcc caactcaaca gatgcactgc tgctgggtcg ataacagcca gtgaaaacga
    11341 aattcccgcg catagcaaca tgacgattaa aaatcccaga ctcatccctg ccagcacgcg
    11401 agtactctga cgaaatccgt gcgttgtggc tgagctaagg gcgagaatat tgttcggtcc
    11461 tggcgtcatg gcggtaatca gggtgtaagt ccaaaaagca cttaaaaggg tcggtgtcac
    11521 ttgttatctc ctctgttttg gtagacagta cccgacacgg aatatttaaa aaaaatgaat
    11581 gtttttacat tctaaattcg atattttcga taggttt
//
LOCUS       DAZGJT010000124        11559 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZGJT010000124 DAZGJT010000000
VERSION     DAZGJT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.124"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..310
                     /locus_tag="ACHKFK_004068"
     CDS             122..310
                     /locus_tag="ACHKFK_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO9054295.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPALSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
     gene            431..1171
                     /locus_tag="ACHKFK_004069"
     CDS             431..1171
                     /locus_tag="ACHKFK_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9054296.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWPVTDETMRNWLKQAV
                     KRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(1450..2427)
                     /locus_tag="ACHKFK_004070"
     CDS             complement(1450..2427)
                     /locus_tag="ACHKFK_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO9054297.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(3267..3908)
                     /locus_tag="ACHKFK_004071"
     CDS             complement(3267..3908)
                     /locus_tag="ACHKFK_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054298.1"
                     /translation="MNADTWTRLQAFRHALELTSCEASLTAGYDHLKDFPAGCSELAS
                     QTLTDYLTEDGSNLYSCIVGMQWDNGPGRYGHVIAAPARDYIDLTLDQFPGYHNRIAA
                     EPVESGGQLAADLNREPAISTADGIVASASDKVNYITEQKNRQPMVIITECYKLIRMT
                     ARFIPMCQSSQLFQHQIFPRLFIISDVGSCSLSRNTLDRLATDWKVTLSVLSL"
     gene            6316..6534
                     /gene="ccdA"
                     /locus_tag="ACHKFK_004072"
     CDS             6316..6534
                     /gene="ccdA"
                     /locus_tag="ACHKFK_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HFO9054299.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERW
                     KAENQEGMVEVARFIEMNGSFADENRDW"
     gene            6536..6841
                     /gene="ccdB"
                     /locus_tag="ACHKFK_004073"
     CDS             6536..6841
                     /gene="ccdB"
                     /locus_tag="ACHKFK_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HFO9054300.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            6842..7651
                     /locus_tag="ACHKFK_004074"
     CDS             6842..7651
                     /locus_tag="ACHKFK_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO9054301.1"
                     /translation="MSGSVIHSQSAARVPAVYSAGQSPQLPVVIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLAPPYPF
                     VQLATLKQRTEKAARTAGRTPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPLERRNKR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAATDGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFSMPESDAVTMLKNRHA"
     gene            complement(7789..8064)
                     /locus_tag="ACHKFK_004075"
     CDS             complement(7789..8064)
                     /locus_tag="ACHKFK_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO9054302.1"
                     /translation="MVKKPSQQALNRAAVTVEQAEALAQRLADKPYGAPEKPEPEKQC
                     RTTISLGESMLVTIEDLALRNKRNGKDPKNVSAIVRVALEQYLKTLT"
     gene            complement(8058..8702)
                     /locus_tag="ACHKFK_004076"
     CDS             complement(8058..8702)
                     /locus_tag="ACHKFK_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO9054303.1"
                     /translation="MITVVGGNKGGSGKTTIASNLAIALANKGREVCLLNGDLQRTAA
                     KHHAEREAAGLLPAITLVEKFDNLTQTLQALNEKFDDVIVDVAGRNSKEFITSGVVAH
                     QIIAPLQCSQPDLDTLTELEQQIDAMRNLNPELKVYCLQSMATTNPVLRGNERKEFLE
                     YLEEFPTIQVLDSVICFRKVYRDCMSNGTGVVETNNTAAKAEIEHLMNEVFGPW"
     gene            8931..9902
                     /gene="parM"
                     /locus_tag="ACHKFK_004077"
     CDS             8931..9902
                     /gene="parM"
                     /locus_tag="ACHKFK_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM
                     domain-containing protein"
                     /protein_id="HFO9054304.1"
                     /translation="MNVYCDDGSTTIKLAWNDNGKICKSLSQNSFRHGWKVDGLGIRQ
                     TFNYELDGKKYTYDEVSNQSILTTHIEYQYTDVNLLAVHHALLNSGLAPQPVSLTVTL
                     PISEFYTKECQKNELNIQRKIENLMRPIRLNKGDVFTIEHVDVMPESLPAVFSRLVVD
                     KVGQFEKSLVVDIGGTTLDVGVIVGQFDSVSAIHGNFGIGVSSVTKAAMSALRMASSD
                     TSFLVADELIKRRNDPDFVRQVINDETKTDLVLNTIEGAIASLGEQVVNELGDFHHVN
                     RVYVVGGGAPLIYDSIKTAWHHLGQKVVMMESPQTALVEAIAAFKEE"
     gene            9907..10299
                     /locus_tag="ACHKFK_004078"
     CDS             9907..10299
                     /locus_tag="ACHKFK_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001555635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO9054305.1"
                     /translation="MDDERKRKKYTLYLHPEKAADFQTLEAIESVPRSERGELFRNAF
                     ISGMALHQLDPRLPVLLTAILSEEFSADQVVTLLSQTTGWKPSQADIRAVLTELGASQ
                     SVEKMPPSATDSVQEAMNDVRLKMKKLF"
     gene            complement(10304..11292)
                     /gene="umuC"
                     /locus_tag="ACHKFK_004079"
                     /pseudo
     CDS             complement(10304..11292)
                     /gene="umuC"
                     /locus_tag="ACHKFK_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766293.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V subunit
                     UmuC"
     gene            complement(11370..>11559)
                     /locus_tag="ACHKFK_004080"
     CDS             complement(11370..>11559)
                     /locus_tag="ACHKFK_004080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054306.1"
                     /translation="CENLSILSFFIFSFSLFFSFRFSSFLVLVFSFLFLFFLQGCAKT
                     PKHFSPQKHPIILPDQSP"
BASE COUNT         2933 a         2592 c         2827 g         3207 t
ORIGIN      
        1 aaagtgactg aatagataac agttcatgca tgaacaagac cataacataa taaaatatgc
       61 ttattatgca taaccttatg ttgtgactaa gccgccttaa aactgagaag tagctaagaa
      121 tatggcgtca gacaccttca gttgtcgtag aaggttatct gcaggatttt tctccgtatc
      181 tgcctgttcc tggcattacc ccaattctcc caaaaacggt aaaccagctc tcagcccgca
      241 ccatgactgg cttaagacgg gatccctgcg gcaaaatgcg cctttacgca tttggtttta
      301 cccgcaataa tgcgtgataa tcaaacaaaa ggatccgcgc gctgcgattt attgtcgtgg
      361 aaggatccgg gcgttcaggt cagaatacct gtatccataa acagtgcgtg tgctacgtga
      421 aaaataactc atgaacaatg tcattcccct gcagaattca ccagaacgcg tctccctgtt
      481 acccattgcg ccgggggtgg attttgcaac agcgctctcc ctgagaagaa tggccacttc
      541 cacgggggcc acaccggcct acctgctggc cccggaagtg agtgcccttc ttttctatat
      601 gccggatcag cgtcaccata tgctgttcgc caccctctgg aataccggaa tgcgtattgg
      661 cgaagcccgg atgctgacgc cggaatcatt tgatctggat ggagtaagac cgtttgtgcg
      721 gatcctgtcc gaaaaagtgc gtgcgcgacg cggacgcccg ccaaaagatg aagtgcgcct
      781 ggttccgctg acagatataa gctatgtcag gcagatggaa agctggatga tcaccacccg
      841 gccccgccgg cgggagccgt tatggccggt cacggatgag acaatgcgca actggctgaa
      901 acaggcggtg aagcgggctg aagctgacgg cgtgcatttt tcgattccgg tcacaccaca
      961 taccttccgg cacagctata tcatgcacat gctctatcac cgccagcccc ggaaagtcat
     1021 ccaggcactg gctggtcaca gggatccacg ttcgatggag gtttatacca gagtgtttgc
     1081 actggatatg gctgccacgc tggcagtgcc tttcacaggt gacggtcggg atgctgcgga
     1141 gatcctgcgt acactgcctc ccctgaagta acggcacttc cttatataag cagtggtcag
     1201 cagaacagac ataacataag ctggagcagg tagataagct gtagtgagta aaccatgttt
     1261 tataaaggga gaaatgcctc agcatcaggt tacggggtga ctcacgtaag ggacaggcag
     1321 atggcagctc agccacaggc agcactgcag gaaactgaat ataaactgca gtgagcagac
     1381 cactcactgc acctgataat ataagctgta gtcagtaaag gagcactctt cactgactac
     1441 agtttatgtt cagcgggatt tgaagagttt ttccaggtca tccagcgtga tgcccagttt
     1501 ttcaagcagg gcaagtttct ccgccatctc tggactgact ttttccgcag gtgaaggtgg
     1561 caacggattt tccttactct catcattcgg tgcttttaac cggggacgcc ggtagtgaat
     1621 gcagaagagt tttgtccgcc cccgctggat ctccgtgtaa tcaagatatc caatctcgcg
     1681 cagctgctcc attgcccgtc tgaccgtctg gttctgggaa aatacaggag acttcagatt
     1741 gaggcgtgca cgcagccgcg ccagcgatat cggtgccgga tcccggggca ggctctctat
     1801 aaaggtgtag agtgcctggg cggactcccg tcgcttcagg gcattaatgg ccttaagctg
     1861 aagaaggacc tttctgtcaa actggtacag ttcaaaaagg cggggatcag cctgtaactg
     1921 aacaatatcc cgttcagtat cgtagtaggc tgactgtacc agatgggtga tgtattcccg
     1981 ggtgtgcttc tcatcggtgc gggaaaagga gatcacggta ccggcaatgc gtttcaggga
     2041 agggctgatg cgctcacgca gcctgcgtga tgactggctt gagggtatac cacacagttt
     2101 tgcaaactcg acaaaaggca gttcaacttt gtcaccaatc acgttatggc gggcaaagga
     2161 atgaatgatc cccacccagg tcttgaaatc gttatccata tccagccggg ggccggtgat
     2221 ctcaacctta tcgaatcctt ctgcacgggc cagagaaaga cgcgtcagtt cttccgtggc
     2281 atcggtgcgt gacagtgtat tttttttact gttcttcagt gacttaaggg tcggtacaaa
     2341 gacgcccaga cgcatcagcg ccacaggttg tacggtgttg ttattgtttg gtgtcagtgt
     2401 caccagctca ccggacgact tatccacctc gccgaacagt tttttgatgt ctgaattttc
     2461 gttttccaga aagcctcctg cctgtggaca gtaaaagaat aaacagaaaa tcgtgtgtca
     2521 gaaccccgtc aactgactga agaaaaacct gcagtctgta tttaacaact gagtctggcg
     2581 tactgaccat agtttatgtc attaacttac ttcagtatat gcattactga atacagctta
     2641 tcatgaacct gttacagttt atccataaca tgccacagct tatgtgaacg tggtcccggc
     2701 ccatgagtga caaggtttgc agcgatatgg ggatctttaa tggatccttt gttgatcatt
     2761 catggatctg tttactggat cgggcctctg gatatgtgga taaatcattc cagaaaaaca
     2821 acatacacta ccgattatga aggcttcagg gcgtacctgc tcactgtaac tacagatata
     2881 ttgcgcttcc tcgccggttc gcgggcttcg cccggtcacc ggtctgcggc gagcaccgtt
     2941 ttttctgaag tctgtcttta caggtaattt ttgacggaat ctgccgtact tcctgagcgt
     3001 acagtcatat ccgaaataca cttcatatgt ttgtttcaac gccagaggcg catacagact
     3061 ggctccatga atatcctgat gacaggcact gctgcgcaga caccggtcat ggatgtaacg
     3121 gaaataccag ctgaaccgca ggtcttccag tcggggacac tgagtacagg gctggagctt
     3181 gtggatacgt atctctggat cttcaaacac tttatggaag gaaaggtgtt aaccagatcc
     3241 cttgcctgcc tggtttatat caagccctat agcgacagga cggacagtgt caccttccag
     3301 tcagtagcta aacgatctaa ggtgttcctg gacaaactgc aggagccaac gtctgaaatg
     3361 ataaataagc gagggaatat ctggtgctgg aagagttgag atgactggca cattggtata
     3421 aatcttgccg tcattctgat cagtttgtaa cattctgtaa tgatcaccat tggctggcga
     3481 tttttctgtt cagtaatgta attaacctta tctgatgcgc tggccactat tccatcagct
     3541 gtactgatgg caggctccct gttgaggtct gccgcgagct gtccgccgga ctcaacgggc
     3601 tctgccgcaa tccggttatg atagcctgga aactgatcaa gggtcaggtc gatatagtcc
     3661 ctggcagggg cggcaatgac gtggccgtat ctgccaggac cattatccca ctgcataccg
     3721 acaatacagg agtacaggtt gctgccgtct tccgtcagat aatcagtcag cgtctggctg
     3781 gccagctcgc tgcaaccggc cgggaaatcc ttcaggtgat catacccggc ggtgagagaa
     3841 gcctcacagg aagtcagctc cagagcatgt cggaaggcct gcaggcgggt ccaggtgtcc
     3901 gcattcactt tttcaccttc ccgtcatgac gggccaggga ctgaagttta cggacgtcct
     3961 cccgggatcc tttcagctca gctttcgtat ccagcagctg cgccgccagc ggtctttttc
     4021 ccgggagccg actttcctgg cacatattct tttcgcagtt atccccagaa tctgtggata
     4081 actgttgctc ctcttttgag agagaaggct ttactcaccg cctgaatgcg gcagatatgt
     4141 ctggacgata tatctgcata cgccggtact gcttcgtcag attgcacggc aggtgaattg
     4201 ccgggctggt accataccat atttgcgccg aacccgtccg gggcggtgcc gataacagat
     4261 gttaaatgat tggtcgtttt tacatctgta aaagcaacag ggccccatga ttatatcatg
     4321 gggccctgtt gctctgattc ttcgcaatag attgctctga attagatgcg ccttctccat
     4381 tagactaaca gttctatatt attttaccat ctttaaatat gtaagagttt tctgtttttt
     4441 attggtgttt atctgtattt tttgttttag gaacgttttt gtaagaatta tcttttatat
     4501 ttgaggtgat atcttattgt ggttgttgta atagtttaga gtcgtaataa atatgattga
     4561 tcgccacttt aaatgaagtt aatctactgg agatgaagta acttcattaa tgcgtcataa
     4621 aaataaaaat tcctgttttg ggaaatatga atgtgtctgt cccctccttc gggagggtgt
     4681 tttttatctg gaaactggtt tctggttctg atttttcttt ttgtattata gcgagttacg
     4741 catatttacc aatcgatgtc tggcaaacag agtatggcga tggtgtgatt tgtgtttatt
     4801 ggtaaaaaaa aacacccccg tagtgggggt gttgaaactt caacctatgt actcattatc
     4861 ctgggatgga tgagtagcgt gcgatattga atcatattca gcaggaataa taatattcgc
     4921 aaaaaaatgg atgtgcaagg aaatttttta tatgcttttg tgtgtggtgg ttaggtaatc
     4981 aagaagataa atgatgaagt gtgccagttc aggatcaatg tttccatggg aaagccattt
     5041 attaataata tcaattaagt tcgaaagctc agggaagatt tgttcaatgt tttcctctgt
     5101 ctcccgcaca gtcatatagc gacgtctcct tggggattga aaacaaaaac aataatacaa
     5161 caagtatgaa acagaaggta ggtggcaggg gcggaacagg aaaacgcatg agggcgcttc
     5221 ctgggggaga tgcctggtat ctttagggtt cagttttgag atcgggaatt tttgtacatt
     5281 aagtgcatta tttaatcaat tgaaacgcgt aatggattga ttttatcagc aactatcgcg
     5341 tttaagttgt cgatcatatt tgagttgtct cattaaggtc gaatcccctg aaagcggcgt
     5401 ataacgtagg agttcccctc tatagcaata ggtaaggctg ttcaaaaaag gcttaccgta
     5461 caataattcg ctatatccag acggcccccc ttgtcataca agctcgtaag cggccctgac
     5521 ggcctgtcaa tgcggtactg ccctgtcggg actgctctac ccacataagt ttctacatag
     5581 cttgaagctg gatggggagg tattacggct ttagccgtaa tactttcttc gtcctcgctg
     5641 cgctgacagt tttcagggat caaatttagg tccgccatac actccgtaaa gactctgtca
     5701 gttcacaggt attacctagt gaagacgtat cataaaatga aaactgtagt tgggtagttg
     5761 ctccccctca cggcatgcag gggctgaggg ggaaaatttc cttatcttgt tgttgctatt
     5821 tctggcttag tcagttttga attttgcttc ctaagcaata atcccctcac cgcaacaatc
     5881 gttcttctac actaataaga tccgaaaaaa ggtagtcacg gatcttcctc tctcgccgca
     5941 cagcgccatc tcccgtcagc acaacatgca gtgcttaatt tagctgctaa tgacactata
     6001 tgttgtgtca tctccctgac ctatgatgcg ccgcgcaggg gcggaaaaca gtgatatgat
     6061 gattttctca gcgtggtaca cttccggaaa gttgtgatat tcatgaaagt cggatctgac
     6121 ggaaacggga atgcgataat tacggtttgt ttttataagt cagattttga tttttattgg
     6181 ttaacataac gcaaggtaaa ataaataagg cttactaaaa gtaagataac agtatgtgta
     6241 tttacgcgct gatttttgca tcataataat atatactcat atacataccc gaagtatgtc
     6301 aaaaagaggt gtgctatgaa gcagcgtatt acagtgacag ttgacagcga cagctatcaa
     6361 ttgctcaagg catatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa
     6421 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggttgaggtc
     6481 gcccggttta ttgaaatgaa cggctctttt gccgatgaga acagggactg gtgaaatgca
     6541 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgttcagag
     6601 tgacattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cccgcctgct
     6661 gtcagacaaa gtctcccgtg agctttaccc ggtggtgcat gtcggggatg aaagctggcg
     6721 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga
     6781 tctcagtcac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
     6841 aatgtcaggc tccgttatac acagccagtc tgcagccagg gtaccggcag tgtattctgc
     6901 cggacagtct ccacaacttc ctgttgtcat tgattatccg gcagctctgg cacttcgcca
     6961 gatgtcgatg gttcatgatg aactgccgaa atatctgctg gctccggaag tgagtgccct
     7021 gctccattac gtcccggatc tgcgccgcaa gatgctgctg gccacactgt ggaacaccgg
     7081 tgcgcgcatt aatgaagcac tggcgctgac gcggggagat ttttcgctcg cgcctccgta
     7141 tccgtttgtg cagctggcca ctctgaagca gcggaccgaa aaagccgcca ggacggcagg
     7201 aagaacgcct gccggacagc agactcaccg gctggttccg ctctccgact cctggtacgt
     7261 cagtcagctg cagacgatgg tggcaacact gaaaattcct ctggaacggc gtaataaacg
     7321 aacaggcagg acagagaaag cgcggatctg ggaagtgacg gacagaacgg tcaggacctg
     7381 gattggggag gcggttgccg ctgctgctac tgacggtgtg acgttctctg tcccggtcac
     7441 gccacatacg ttccgccatt cctatgcgat gcacatgctg tatgccggta taccactgaa
     7501 ggtcctgcag agcctgatgg ggcataagtc catcagctca acggaggtct acacgaaggt
     7561 atttgcgctg gatgtggctg cacggcaccg ggtgcagttt tcgatgcctg agtccgatgc
     7621 ggtcacaatg ctgaaaaaca gacacgcata attcataaat cataattatg aattgtgatt
     7681 tattctgtaa aaaaagagac cactgcaata tgtgatctct tgtatgcaag ggtgcttaaa
     7741 cagtatgaat tcacaagtgt gacttcataa gtaataactt attgattatt acgttagtgt
     7801 ttttaagtat tgctcaaggg ctactctgac gatggcactt acattttttg gatctttacc
     7861 attgcgttta ttcctcaatg ccagatcttc tattgtgaca agcatgcttt caccaagaga
     7921 gattgtcgtc cgacattgtt tttctggttc gggtttttcc ggcgcgccgt atggtttatc
     7981 cgcaaggcgc tgagccagag cctctgcttg ttctaccgta actgcagctc tgtttaatgc
     8041 ttgctggctg ggtttcttca ccatgggcca aatacctcat tcattaaatg ttcaatttct
     8101 gctttggctg ctgtattgtt cgtttctact acaccggtgc cgttgctcat gcaatcgcga
     8161 tagacttttc tgaaacaaat aactgaatca aggacttgaa ttgttgggaa ttcttcaaga
     8221 tattcaagga attcttttct ctcatttccc ctcaaaaccg gattggttgt tgccatactt
     8281 tgtaaacaat aaaccttaag ttccgggtta aggttacgca ttgcgtctat ctgttgttct
     8341 agttctgtaa gagtatccag atctggttga gaacactgta gtggcgcaat tatttgatgt
     8401 gcgacaactc cactcgttat gaattccttt gagtttcttc ctgcgacatc cactattaca
     8461 tcatcgaact tttcattcag agcctgtagt gtttgtgtca ggttatcaaa tttctcaaca
     8521 agggttattg ctggtaacag tcctgcagct tctcgttccg catgatgttt tgcggctgtt
     8581 cgttgtagat ctccgtttaa caggcagact tcccttcctt tatttgcgag agcaatagcc
     8641 aggtttgatg caatggtagt tttccctgaa ccacctttgt tcccaccaac tacagtaatc
     8701 attgcaacct cataaatgtg atgtgtgaag tatgatgata ttttgacacg gtaacctgag
     8761 gtgtcaatat gtattcatat ttatgaagta tgaaatatga agtgtgtcat gtgatgtaat
     8821 aaataatact tgtgttttgt gatttcataa ctatgatagt gctcaaattg agtattacct
     8881 tgcaaccaga aacagtgatt tactcagatt gagtatttaa ggggctatag atgaacgtat
     8941 actgcgatga tggttcaaca acaatcaagt tggcatggaa tgataatgga aaaatctgta
     9001 agtcgttgtc gcaaaactca tttcgtcatg gatggaaggt tgacgggtta ggaattcgcc
     9061 agacatttaa ctatgaactg gatggtaaaa aatacacgta tgatgaagtt agcaaccaat
     9121 ctattcttac gactcatatt gagtatcaat atacggatgt taacctgtta gctgtgcatc
     9181 acgctttact gaatagtgga ttagcgcctc agcctgtatc attgacagtg actttgccta
     9241 tcagtgagtt ctatacgaaa gagtgtcaaa aaaatgaact caacattcag cgtaaaattg
     9301 agaatttaat gaggccgatt cgccttaata aaggcgatgt tttcaccatt gaacacgttg
     9361 atgtgatgcc tgaatcattg ccagctgtgt tttctcgtct ggtcgtggat aaagttggtc
     9421 agttcgaaaa atcgctggta gttgatattg gcggcactac gcttgatgtt ggtgttatcg
     9481 ttggtcaatt tgactctgta agtgccattc atggtaattt tggcattggt gtgtcttcag
     9541 ttacgaaagc tgcaatgagt gcattgcgca tggcatccag tgataccagt ttcctcgttg
     9601 cagatgaatt gattaaacgt cgtaatgacc cggactttgt tcggcaggtt ataaatgacg
     9661 aaacaaaaac tgatctggtc cttaatacca ttgagggggc tattgccagt ttgggggagc
     9721 aagtagtcaa tgagctcggt gatttccatc atgtgaatcg tgtttatgtt gtcggtggtg
     9781 gggccccgtt gatctatgat tcgataaaaa cggcatggca ccatcttggg caaaaagtag
     9841 tgatgatgga gtcgccacag acagcactgg ttgaagctat cgcggctttc aaagaggagt
     9901 aatattgtgg acgatgaacg gaaaaggaaa aaatacacac tttatctgca tccggaaaaa
     9961 gctgcagact ttcaaacttt ggaagcgata gagtccgttc cgcgttctga gcgtggtgag
    10021 ctgttccgga atgcatttat ttctggaatg gcgttacacc aacttgatcc tcgcctgcct
    10081 gtattgctga cagccatttt gagcgaggag ttttcagcag atcaggttgt aaccttactc
    10141 agtcagacga ctggatggaa gccttctcag gccgatatca gagctgtgtt gactgaactc
    10201 ggtgcttcgc aatctgttga aaaaatgcct ccttctgcta ctgattcggt gcaggaggca
    10261 atgaatgatg tgcgtcttaa aatgaaaaaa ttattctgat atatcagaat gaagcaatgg
    10321 ggatctcagt ccattgtgtt gtatacgaag gcgatagcat ttcccgtttc atttgccatt
    10381 caggggcggt tcctcgcccc gcaaaccaca ctttcccttt tcccgactgg ttcagttcat
    10441 cgagtgtttt cattaacttt tcactgtttt tacggggctg gatttcatca aataatcccg
    10501 gctgcgctat acccgatggt gcgaaatcag ccagcatgac tcctgccttc atatagcggt
    10561 acccttcacg ccagacatgg tttaaggctc tgcatgcggc ggcaataatg tcccggctgt
    10621 cctgtgtggc aatgaaagct tttccacagc ggcattgctg taacagggtt cttttactgc
    10681 aaagggggat gtccgtacaa atgtcgtcac ctgccgacaa tactgacgct ccccacgtag
    10741 tttctctgcg gcccgctctg catactgaac aacagcctgg tgcatggcat ctttgtctgt
    10801 gattcgttca ccaaaactgc gactacagac aatctgctgt tttgccggtg gtgcttcttc
    10861 cagggatatg ctggactcgc cgttgagttc gcgtaccgta cgctcaagaa tgacgctgaa
    10921 gtttttccgg atgaatgccg tgttagcctg cgccagttgc agtgctgtgt taatacccag
    10981 cgcattcagc ttttccgtca gtctgcgtcc tactccccag acctcaccaa ctggctgcag
    11041 ccccagtagc ttcaagatcc aattacggtt ttctgccgtc agcgcgacca caccggaaaa
    11101 ctgtggccat tgctttgttg cccactgtgc acttttagcc agcgtttttg taggcgcaat
    11161 gcccaccccc atggtgagtc ctgtccagct ctttacctgt tccctgagct gatgaccaaa
    11221 agcctccgga gagatgcaat gatttatccc ccgaaaatca atgaacattt catcaattga
    11281 gtagggctca acttcctgaa tacggtagtt atcaggaatg gtgcggggtg gctgggtgtt
    11341 agtgggatta tatccggcag tgtatccggt taaggtgatt ggtctggcaa gatgattgga
    11401 tgcttttgtg gtgaaaaatg ttttggtgtt ttagcacaac cctgaagaaa gaataaaaat
    11461 aaaaaggaaa aaaccaaaac cagaaaagaa gagaaccgga aagagaagaa aagagaaaat
    11521 gaaaaaataa aaaaagaaag aatacttaaa ttttcgcat
//
LOCUS       DAZGJT010000125        11491 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZGJT010000125 DAZGJT010000000
VERSION     DAZGJT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11491
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.125"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..886)
                     /gene="uxuR"
                     /locus_tag="ACHKFK_004081"
     CDS             complement(113..886)
                     /gene="uxuR"
                     /locus_tag="ACHKFK_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO9054307.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNADSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKKRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(1101..2561)
                     /gene="uxuB"
                     /locus_tag="ACHKFK_004082"
     CDS             complement(1101..2561)
                     /gene="uxuB"
                     /locus_tag="ACHKFK_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO9054308.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGNETIADTMTNPAYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(2642..3826)
                     /gene="uxuA"
                     /locus_tag="ACHKFK_004083"
     CDS             complement(2642..3826)
                     /gene="uxuA"
                     /locus_tag="ACHKFK_004083"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO9054309.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            4275..4382
                     /locus_tag="ACHKFK_004084"
                     /pseudo
     CDS             4275..4382
                     /locus_tag="ACHKFK_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4625..5527)
                     /gene="fimH"
                     /locus_tag="ACHKFK_004085"
     CDS             complement(4625..5527)
                     /gene="fimH"
                     /locus_tag="ACHKFK_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO9054310.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYNGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGVVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(5547..6014)
                     /gene="fimG"
                     /locus_tag="ACHKFK_004086"
     CDS             complement(5547..6014)
                     /gene="fimG"
                     /locus_tag="ACHKFK_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO9054311.1"
                     /translation="MLALASATIQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSF
                     SLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAVDSTGYYKNQGTAQNIQLELQDD
                     SGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(6063..6593)
                     /gene="fimF"
                     /locus_tag="ACHKFK_004087"
     CDS             complement(6063..6593)
                     /gene="fimF"
                     /locus_tag="ACHKFK_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO9054312.1"
                     /translation="MRNKPFYLLCAFLWLAVSRALAADSTITIRGYVRDSGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(6603..9239)
                     /gene="fimD"
                     /locus_tag="ACHKFK_004088"
     CDS             complement(6603..9239)
                     /gene="fimD"
                     /locus_tag="ACHKFK_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO9054313.1"
                     /translation="MSYLNLRLYQRNTQCLHIRRHRLAGFFVRLFVACAFTAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSMIHDATAQLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDSSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(9305..10030)
                     /gene="fimC"
                     /locus_tag="ACHKFK_004089"
     CDS             complement(9305..10030)
                     /gene="fimC"
                     /locus_tag="ACHKFK_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO9054314.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMVVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(10067..10606)
                     /gene="fimI"
                     /locus_tag="ACHKFK_004090"
     CDS             complement(10067..10606)
                     /gene="fimI"
                     /locus_tag="ACHKFK_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9054315.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(10670..11218)
                     /gene="fimA"
                     /locus_tag="ACHKFK_004091"
     CDS             complement(10670..11218)
                     /gene="fimA"
                     /locus_tag="ACHKFK_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO9054316.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         2781 a         3031 c         2755 g         2924 t
ORIGIN      
        1 tatatcgtac gtaatgatta atttacgcat aatatattct gcagaacata attaactatt
       61 cagggaacat acatagtgca aagcggttaa actttgcacc agaaatgagg gatcaggcgt
      121 cgactttatc cagcggccat gaatcaaaca gatagccatc gaaatagatg tcatcaacat
      181 tagagaactc cagcaagcgc ttcttcacat tctccagatg ctgccacatt gccagcttcg
      241 ccgctcgggc atcttttttg attaacgcgg cgaggatctg tttgtgatcg cccaaccact
      301 ctttgcgata caggctgtca tccagatggc tgtgcaactg gatccacatt ggattgtttt
      361 cccgccactg ccaggactga cggaataact ccaccagcat gctgttatgc gttgcttcgg
      421 caatagcgag atggaactgc atgtcaccgc tttcgctgct gcccggcgcg ctggaagcca
      481 gttcacgctc ttccagttgc aatgcctggc gcattttgac gatatcttcg cgggtagcct
      541 gcagggcggc aaactcggcg atgttacttt ccagtaactg ccgcgcctgt aacagctcaa
      601 aaggaccggc atcgttgcag acgttggcat ccggactgtc tgcgttgtgg ctgcctgagc
      661 tatcaagaac atagatacct gcaccccgac gcacttccac cagccctttg atctccagca
      721 tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag catttctgca atttcacgct
      781 ccggtggcag ccgttcgcca ggattgtacg gcgtctttat gatcagatcg cggatcatcg
      841 ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga tttcatggtg ttgtgtccgt
      901 taggtgcggt taatcaaatt ttttccggat gcggcgtgaa cgccttatcc gacctacaac
      961 gggcaatttt acggcacccg ttaagctcaa actataccat tagcactgga tgttggcacg
     1021 tacaggtggg caaaaaaacg aaaactggtc aaccaaatat tagtttgatt gaccagtaag
     1081 tctgtagggt aatcagttag ttacagcgca gccacacact cgcgcgcacc gcgctcacac
     1141 agctgctgat atgccgccgt caccgcgccg acaaagtcgg cgttctgcgg cagatcatcg
     1201 gcaaagatac cgctcaggcc cagcagtgct ttcacgcggt ctgcaccctg atactgcgcg
     1261 ttgatcttct ggaactccgc cagcatcggg tcaaccacgt caatggcatt accctgctca
     1321 tccacgccct gggtgtagcg catccagccc gccacgccca gcgccaggtg gcgccagcta
     1381 ccgccgtttt gcaggtgcag acgcaccggg tccagcagac gctgcggtaa cttctggctg
     1441 ccgtccattg caatctgcca ggtacggtga cgcagagacg ggttgctgaa acgctcgatc
     1501 agcagcgtcg cataggcgtt caggtccgta ccttccggca tcgacagcgt tggcgcttgt
     1561 tcctgcatca tcagggcaaa ggccgctttg cgataagccg ggttagtcat ggtgtcggca
     1621 atagtttcat tgccgccgag gtaaccgagg tacgccagga aagagtggct accgttcagc
     1681 atacgcagct tcatcatttc gaacggtaca acatccgcaa caaactgcgc gccgacttta
     1741 tcccagtccg gacgaccatt aacgaagtta tcttcaatta cccactgacg gaacggttcg
     1801 caggcaatgg cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct
     1861 ggcgtcgccg ccggaacgat gcggtcaacc atggtgcacg ggaaggtgac gttttcttca
     1921 atccatgccg ccagttgcgg gtcacgcgcc tgagccagcc caagaacggc aaccttcgcc
     1981 acatgaccgt tttcacgcac gttgtcgcag gacatcaccg taaacgcttt cagccctttt
     2041 tcacgacgca gacgcagggc ttctacgatg taaccaatcg cagacttcgg cgcagtcggg
     2101 ttttcgagat cgtgcttgat cagcgggtta ttgagatcca gctgaccgct tgccgcatca
     2161 gcgcagtagc ctttttccgt gaccgtcaga gagacaatcg ccgtctgcgg acgcgccatc
     2221 gcgttgagaa taccttcgca accgtcgatt tccggatgca gcgcttcttt catcgaaccg
     2281 ataattttca gctcggtgct ttccgcgcct ttttccgcca cggtgtacag cagttgctgt
     2341 tttttcaggt tttcgatcag cacgcggtcg ttgcctggca tcaggttaac ttcgcagata
     2401 ccccagtcgc tgtcggtact ttccagcaga tgatgggtat acagcgcctg gtgcgcgcgg
     2461 tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca gacgagaatg atcccatgac
     2521 gggcgggcaa ccggcagatt gctgtcaaca atagtagtca tcgtaaactc cagaatccgt
     2581 cttcagagag tgacggcaag gaagaaggaa ccggcacggc gcgcagccat gccggtgggt
     2641 attaacggct aaagaaagcg cgctggatcg ccagttcgac accgcgaact tcggccaggc
     2701 ctttcagacg accaattgcg gagtaaccag gattggtttt cttcttcagg tcgtccagca
     2761 tctgatgacc gtggtccgga cgcatcggga tcaggtcttc tttgccttcc gctttacgac
     2821 ggtgttcttc ttcaacaatc gctttcacca cttcgtacat atcaacgtca ccgttcaggt
     2881 gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc agatgggtga
     2941 agtaaatacg cggaccgaac tgcttgatca tatcaaccag atcgttgtca gcacgcacgc
     3001 cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct gtttacggta tcaaccatcc
     3061 actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc gaggatcggg cgcggcggat
     3121 cgtccgggtg aaccgccata cgcacgccaa cttcttcagc aactggaata atcgctttca
     3181 ggaagacagc aaagttttcg cgcagtttgg ctttgtcgat atctttgtac agctccagat
     3241 gcttacggaa ctggtcgagg gtataccctt cttccgcacc tggcagaccg gcaatgatgt
     3301 tacgggtcag acgcgctttg tcttcgtcgc tcatagtggc gaagcgttca gcggcctgag
     3361 caatttcttc ttcggtgtaa tccgcttccg cgcctggacg cttcaggata tgcatttcga
     3421 atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga gccgtctggc agcacgtatt
     3481 cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc
     3541 cgcactgcgc caggttgcgc agggtctgct gatagttagc aatccactgc tcatagttgc
     3601 cagtgtgggt tttgatatct tcgtgaattg gcacgctttc tacgacagac cataccaggc
     3661 ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc aacggaccat acttcgccat
     3721 tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta
     3781 aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg ttccatgatt tttcctcttt
     3841 cacatagtgg gttcaacagc gaagagacgc ggaaccccgg agttgtttag gtatccggat
     3901 gaacaacccg gccacagccg tagcgtcgca tcggatgaga tggcgtataa gttctacctt
     3961 ttggtaagac atgtcaaccc gaattctaaa attggttaac cacatcacaa gaatttaact
     4021 ctcctggttg ttgctgcaaa ttgcagcaaa gggtaatcga tggttattga tcagaggatg
     4081 tgacattcac cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa aataacaacg
     4141 gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac attctctggg tggtattcgg
     4201 cattggcctg atgctggtac tgacaatgac cgcaggttca gacacattcc ctacattagc
     4261 aatgcctctt agattacttt gacctgtcag taaaagacac gttgaaaacc tgaggactgc
     4321 tggagctggt caactccgtg gttgggctga ttattgtgtt gattattagc atggtagcgt
     4381 aaaagagcac cagagcctgc cattggcagg ctctgactga tgacaaagtc taaaaatgtg
     4441 cccggccagt cccctcgccc ctccggggag agggttaggg tgaggggaac agaccagcaa
     4501 agtgcgcttt gtttatgcca gatgcgacgc tgacgcgtct tatctggcct acaaagggct
     4561 aacgtgcagg tttttagctt caggcaatat tgcgtacctg cattagcaat gccctgtgat
     4621 ttctttattg ataaacaaaa gttacgccaa taatcgattg cacattccct gcagtcacct
     4681 gccctccggt acgtgcgtaa tttgccgtta atcccagact taccgccgaa gtccctacta
     4741 ctcctaacga taccgtgtta ttcgctggaa taatcgtacc gttgcgcgtc aactgtacgc
     4801 cgacgccctg cgctggtgaa aacgacgcgg tattggtgaa aattgagttg cccgcatctg
     4861 cggttgtgcc ggagaggtaa taccccaggt tttggctttt cgcacaataa acggtaagag
     4921 gaatcggcac tgaaccaggg tagtccggca gagtaacggt gacatcacga gcagaaacat
     4981 cgcagccgcc agtgggcacc accacatcat tattggcgta aatattccac acaaactgga
     5041 aatcatcgct gttatagttg ttggtctgtc gcaaaataag cacggcaatt aatgagccag
     5101 ctttaatcgc cactcccccc gcactgctca caggcgtcaa ataaagcgcc accggccacg
     5161 gcttatccgt tctcgaatta taaacaaccc gcggcgtttc gctggtagta gggaaaggat
     5221 agctactgcc attatatttt acggtcccgg aaaaattaga taacacgccg ccataagccg
     5281 agcctcgttg cagtgtgaca taatctgtaa tggtttccgg gtaatcgtta tggcaaaaga
     5341 tttgcgtcga aagatccacg accaggtttt gccccacatt cacggcaggc gcaaggttta
     5401 cataaacatt ggcgctgcca ccgccaatag ggattgcggt accattggcg gttttacagg
     5461 cgaatgacca ggcatttacc gaccagccca tcagcagtac agcaaacagg gtaataactc
     5521 gtttcattac aatcatctct ttgggttcag ctgtaggtat aggtgatgct aatcactgcc
     5581 tgaatggttc cctgagtggc tccgccattt accgtcaatg ctctgacctg taacgggaag
     5641 tgcgctgatt gtgaggaatc atccacctga actgttttgg ttgcgccagt attcaatgtg
     5701 ttgccactgt catcctgtag ctctaactgg atgttttgcg cggtcccctg gtttttataa
     5761 tatccggtac tgtcgactgc cccgctgaag ctggcagtga ccctcgacgt tcccaccgga
     5821 caattagtca actcaagcgc aacatcatgc caggccgatg ccgccccggc agacataaga
     5881 ctgaaagaat aaagatcgcc gagatctacc gtggcattgg tggtggaaac cgtgcacggt
     5941 ttggcgacga ccttaccgtt caccgtgatg gtgacatcgg ctgcctgtat cgtcgcactt
     6001 gcgagcgcca acattgccgc caataaatac ccacgttagc accatttcat gagcatctcc
     6061 agttactgat attcaagagt gaaggtagcc gtggcattga tatgccccgc agtgacaggc
     6121 acctgtgtcg ccattagccg ggcgtaaaaa ttcagcgtat ttggtttacc cggcgtcagg
     6181 gtcgtccacg aaatcgcgga cgatggagca ttaaggggta tttgattttg ctgctcattc
     6241 agaagctgta ttcccagtcc cgcagccgct gacaccgtat tttcaagtgc aagcaggttg
     6301 gcattgtggc tatctgcaac gccagtaaac ccaaccttta cggcagaaac ggcgttacca
     6361 cagggtgaca gcaaaatacg aaatggaacg acaggagtcg tcgcgccaat gttgttaaat
     6421 tgcttcgccg cgttttccat cagatcaaca gtaaaattgg ttgattcagc ggccacacta
     6481 cagccgctat ctctgacata gccgcggata gtaatcgtgc tatccgcagc caaagcgcga
     6541 ctcaccgcca gccacaaaaa agcgcacaga agataaaaag gtttgtttct catcacgccc
     6601 ccttaacgac attcagctga tagctgggtt aataactgct gctgactctc tggtggcagt
     6661 tgataattgg cgacacagtg agcattttcc tcttctcccc atttcacctg aacttttccc
     6721 gctaaaggca ttccgctgag gtaaacctga ccattatccg caacaatgcc actactctgg
     6781 ctactctctg atgtcaccat cgccccaaac ggcagcggct tattattgtg ggttagcgtc
     6841 atgagcagtt ttatcccaac gcgcgcttta aactctgctc gcacgatcgc cccacgggtg
     6901 ggaacaacgt tagcgaccgc attatctaaa tcgacgttat cagccagggt attggtatcc
     6961 agcgccactc tgttttcccg atattcagtg gcatatggca gcacggcata accccgccag
     7021 tcggtacgca ctcctgtctg gttttcgact tttgcatctt ttgcgccagg cgctttaaca
     7081 agcaccaccg tatcgtttaa cggctgcccc agcgttacgc cattggcatg agccagtacc
     7141 ccaccgctga ctccgtaata gagctgctta atatcatcgc tatggctgta accgatattg
     7201 gcattgccgt aaccaccgcg ataattcagc gtggcgtagc ctgtacttcc gctatttcca
     7261 tcgcctcccc cggcataacc ggtttgcacg ctatagctga ggttgttgtc ttccagcaac
     7321 gtaccgtata caccggccag attggtcatc cgaccgttga gatcgtgtga catgctgtag
     7381 ctggcactgg catgtcgcca ctgagattta ctgtcagaac gcagccagtg gctgaaagga
     7441 atattgacgt taagcgctaa catctgatcc cgtccttttt gccaggcgtt tttcgtcagg
     7501 ctatagctga gcgtccagtt aatatcttcg aacgcagtat ttaatccagc ctggaattgc
     7561 tcatcgacat tactcgttcc ccaataagtt tgatggctac cactcaaata cagtgttgat
     7621 gtgcgcccga gttgctgagt aacggtgagt tgtaatttcc cgcgtttgtt ataagcgaga
     7681 ttgtaatagt cggtgaattt cggcttaacc tgaataactc catcctgtgt ttcgatgttg
     7741 tagccattca ttcgactgta tgttgtatca gcgaaattaa aatatccact ggtcgaataa
     7801 cggtaaccca ctaactgaat attcgtgcct gactcattga gcgatttgtt ataaagaaaa
     7861 cgcaccgatt gtccgtcatg ctgactgtca tcgggaagtg tggaattagc ctgcgtcata
     7921 tccacagaca gagcgcccag tgcccccatg tttttcccga taccgaaatt aaacgcacga
     7981 taacgatccg caagttgcgt tccaccatat attgtccagc cagccggaag gccgtggagt
     8041 aatgtactct ggaaaaagcg gggtttttcc tgttgcgcat ttccactacg gtattctcct
     8101 gccgtaatgg aataacgtgt atgcccttca cgttgcaaga gcgggactga cgaatagggt
     8161 acggtaaaaa tctgcgtact gccgtcagcc tctttaatcg ttacctgcaa gtcaccacta
     8221 ttacctgcgg catagatatc gttgatggta aaaggccccg gtggcaccgt actattataa
     8281 atgtcatacc cattttgttt aatagtgacc tgtgcagtac cacgagcaat accgtggatc
     8341 accggggcaa atcctctttg gctatcgggt aacatattgt catctgaggc caattgtgcg
     8401 ccgcgaaagt taataccatc gaaaatatcg ccctgagtat aaccatcacc cagcgtcagc
     8461 cgggaacgta acggtattat gtctcgctca agccaggtat tgatatgctg ccatttattt
     8521 ttgctacctg atgaactgtc gctactgtta taactccagg tggtattgtc gcgtaaacgc
     8581 cacgcaccaa tatttaaccc actctgtagg tttaaatatg cataatggct gttaccccca
     8641 attcgattct gtacactatt tccgctgaaa ttataattga gcaatccggc attaataccg
     8701 ggatcccata actcaggagg aatataacca cgcgcgcgat tactcataaa tgcctgaggg
     8761 atcgtcaggt tgagtcgctg ctgacccaca tccagttgcg cagtagcgtc atggatcatt
     8821 gaggttaatg gcacgcaggc atcatccgcc agcagattca taccggagac agaagccgta
     8881 ttcagcccca tactggcaag ttgcgcgcgt gtcaggcagg gaacaatccc ttgttcgctg
     8941 tcgcccgtat taaatgtgac atcacgcgtt gccatataac cattattcaa atagatatca
     9001 acacgatacg tccctggcgg taattcttgc ccattttcaa aacgcgataa atcagccaca
     9061 gcctggggat catccgctaa aaaacgcggg ttaaaataga gttcggcaga tgacaaaggt
     9121 gcctgtgcgg taaaagcaca ggcaacaaag agtcggacaa aaaaaccagc caaacgatgc
     9181 ctacgaatat gcaagcattg tgtgtttcgc tggtaaagtc ttaaattcag atatgacatt
     9241 attcctccgg tttaaatcac ccggcagtca attcttttta ttcaggcgaa aaaatccccc
     9301 tgcgttattc cattacgccc gtcattttgg gggtaagtgc gccataatca tttattgttc
     9361 ggtaagtaat attgcttcct gcatcagaag gcaatttaac cgtgctttcg cccattggag
     9421 gcaccaatgc attttcaaga acccgagttc cggcattcaa ctctgttacc gtcaggtaat
     9481 agggtgtcgg gttaatcagc gtcagagaat tcgcgctacg acgaaatctt aatttttctg
     9541 cggcctgatc gggtggcaac gctaatttag ccggacgata gtacagttta atgcggctga
     9601 taattgcgag ctgtagcgta ttctcagtca attttgattt atccattgac ggaatcgctt
     9661 taacattcat ccagaataaa ctttcccgat cctgtggtaa ttggttattt gttgcatcaa
     9721 gaatacgcaa ggtattctct ttttttcctt tcatcgcaaa cagaggtggc gtcacgataa
     9781 aacggccatc ctttacacca tcggcatttt ctacccatga ttgaattaaa taggtgctat
     9841 tttcatcatt atttgtcacg gcaagttgca cttgtttttg ccctgccgga taaattacgc
     9901 gagtcgcacc taaggccact cccgcttcag cgcgtccggc aaccaccatt gccatgaaca
     9961 tcaggatacc tgccagcaag cagaatgtta tttcctgcga tttccttaca ttgacgtttt
    10021 tattactcac tgtcctgttc ctgttatcac attatctgct gaataattac tgataggtta
    10081 aagagaacca ggcctgggca ttggcgatgc cgccagtaac ccgacgcccg gtagcacgat
    10141 atttggcgat gaaatgtagc gacgtagagc ctgagtaaag ccgtttccag tttactggag
    10201 gacgattaat cggtacgagg tttccttcat catcaaacaa cgctacgcca atattggtgg
    10261 ctatccctgg cccctctccc acggaaagca catccggatt tttaccatcc gcgacaccgt
    10321 gaaacgccac ccccacacgt tcactcacca ccgtgctaca ttcccgtaaa tgaataacaa
    10381 aaggcactgg tgcgctatct tcccctaccg catgaaaccg gttactgctg atttgcccca
    10441 tattgaccgt catttgttta tcgccggctt caatccggca agtttccgca ataatgacgc
    10501 cctgaaattg catatttccg ccaggcagcg tggtattcca tttatttccg gccagagcaa
    10561 acattggcag caacgacgct aacagaaata atctcttcct tttcatcgtt atttttatcg
    10621 cacaagggtg ggcatccctg cccgtaatga cgtccctgaa cctggtaggt tattgatact
    10681 gaaccttgaa ggtcgcatcc gcattagcag cacccggggt tgcctcgccg attgcataat
    10741 aacgcgcctg gaacggaatg gtgttggtac cgttattcag ggttgtttgt gcactaaatg
    10801 tcgcaccgtc cagcgccagc gcagcacccg ttctgtccag aatctgcaca ccaacttttg
    10861 ttgcactacc cgcagctgaa ctctgcaaag ctaatacgtt aggatgagtt ccatcaattg
    10921 cggtaccaag gaaggcaata gctgctttgt ctgcaacagt agtgtcacaa tcgttcagtt
    10981 gaatgttaaa accgacagca gaactggttg ctccttcctg cgccagcgat gcggtacgaa
    11041 cctgacctaa ctgaacggtt tgatcaacag agcctgcatc aactgcgcaa gcggcgttaa
    11101 caacttcccc tttaaagtga acggtcccac cattaaccgt cgtggcagcg gccagagccg
    11161 ctgtggaact gagggacaga gccgacagaa caacaattgc cagagtttta attttcatac
    11221 tgctttcctt taaaaaaact atttctaaat cgacatgggc agtcgttctg tacactttgt
    11281 tttgtcaacg agtttgactg ccaacactgc acagttttcc cccaaaagat gaaacatttg
    11341 gggccatttt gactcataga ggaaagcatc gcgcacaaac tttttcagtt tatttgttgg
    11401 cttaatgttc tatagttatc tttatttgca gttttttata ttgcatgagg tggtttttgg
    11461 agagaagaat gaggaagttg cgtcgagcta c
//
LOCUS       DAZGJT010000126        11285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZGJT010000126 DAZGJT010000000
VERSION     DAZGJT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.126"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1544
                     /locus_tag="ACHKFK_004092"
     CDS             <1..1544
                     /locus_tag="ACHKFK_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO9054317.1"
                     /translation="VADSVVRDCLVSRAIYSDVPEHHLLNVMAYFQAISGFDFAPVYA
                     TYHDRYGDEGLLKRLTDLAFVTRFLRVFRDQRVNEVCRMLGILNRNAPYISDWHRDLF
                     AVRSKRVKKYLRSSGVFDAFNELICTLEDAHNASVSNPKNRIAELCVRGKAVCELSED
                     MGLSGYFIVLTTPSRFHPTTSFKVAGKWHSRPNKKWWEAGCPTVKDSHAWLNTVWRRV
                     CRRLDKAGIQIPGLRTVEPHADGTTHWNFLIYCNPHESATVLAIFREEAMRDEPDEKG
                     AKEHRIRIEAIDPEKGDGFRYIVKYITKMAGDVSADGITALNDRYSARSFCDAVSRAA
                     CWQKATRLRLFQFFGVPSVTAYRQMRTFRSPLEAHHINMQQFTPQQVAELEAIRMACD
                     AGDFRTYILLNGGFFCSERLLRPFYVQPQEGGKPRINRYGEPCAPVISGFMFGPVPVI
                     TRFMGCVVRRMTPAEKIRAEEVRSGSSYESVFISSASRHRTTRSRAAGGGGEADPWTC
                     DNNCL"
     gene            1575..2000
                     /locus_tag="ACHKFK_004093"
     CDS             1575..2000
                     /locus_tag="ACHKFK_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054318.1"
                     /translation="MNQIYDSFPFPSPEGFTSGMTMAGYFIHVTVINDEELKPTDSHY
                     NPEAKPKYAIVIAYPYEDRKLKVRREEHERFSCTEEDFKFFNSCPDLQGKPVYLTVDV
                     NSWSTGSERHGVWYRFISGSMKRFDGQPLGALPAGKEKG"
     gene            2005..2145
                     /locus_tag="ACHKFK_004094"
     CDS             2005..2145
                     /locus_tag="ACHKFK_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054319.1"
                     /translation="MSDADFLNFVLFAAPAMILFSSGFGIGIKLIRACDSQSITVQRY
                     YD"
     gene            2138..2308
                     /locus_tag="ACHKFK_004095"
     CDS             2138..2308
                     /locus_tag="ACHKFK_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054320.1"
                     /translation="MTEFDFSGVLSALNSGVQDGELYWSVLYVWIVCWLLGFGLGVIF
                     TLVCRMFSDAVN"
     gene            2333..2575
                     /locus_tag="ACHKFK_004096"
     CDS             2333..2575
                     /locus_tag="ACHKFK_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054321.1"
                     /translation="MKLKTLVLSGLATTASVAALPASAAVQVPSFITADTVKEIGDAL
                     TSVIGIAGTAAFAVLGVSLAARLGIGIIKGFLSRAT"
     gene            2636..3991
                     /locus_tag="ACHKFK_004097"
     CDS             2636..3991
                     /locus_tag="ACHKFK_004097"
                     /inference="COORDINATES: protein motif:HMM:NF017195.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054322.1"
                     /translation="MIHFIIPLLFLFFSSFSFAEEKENPDALVQCAGHSSCRTDYSDA
                     GTSIKLARAAGHLFCGSGSYDACSGIIHYCGVPLSTPVNAPSTVGSFVRARWSAYKVW
                     DSDKQKWTACTLAFQGDEGDLTPEYNKPPTDEICLSRPVLDGMTFSDVYKGDDGTRYV
                     NVQGCIYEATGNPLICNPDDDTVCTADWKPVAVDPAFSDGKEDGGSSGGGSSGGGNPG
                     GGSSGGGSGSGSGGSSGSGGSGSGSGGSSGSGGSGSGSGGSSGSGGSGSGSGGSSGSG
                     SSGSGSGGSSGDDNGWLDGILGWLKTIWVSVNDLNNAFSVSQADMNNALNSLDNGIKD
                     ISQNIASSLPDGDFPLGELSHYLPGQGSESHDNNNWLTVNIDKLVNTNNGTALPFTLQ
                     FSFNLPLIGAINFKIDTSHFAKAYDDYVRSVLEYAIYMITMLRVFVISRRTLFEREVK
                     L"
     gene            3991..4350
                     /locus_tag="ACHKFK_004098"
     CDS             3991..4350
                     /locus_tag="ACHKFK_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054323.1"
                     /translation="MWAILGAFFVDVVLKSLRFIAGVIGDKAVAWAKLLSYLGLCYTS
                     YYALVSAVNFIIRQISDVNKLDVIVVAFSYLPLNIQYCFNIILHVQFISYIYLYKDRV
                     YRLVYDLFKSNGLNVGK"
     gene            4367..5578
                     /locus_tag="ACHKFK_004099"
     CDS             4367..5578
                     /locus_tag="ACHKFK_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HFO9054324.1"
                     /translation="MPVITGNLGQGKGIVAAYFASLYYRRGLRVAANFPLNTEYMSSG
                     SDNPVTVIPAMPRIEDFELLGRGCPENEKTRFGALFLDECATWLNTRGFARKDRLPLI
                     DWLIHSRKLGWDVYLISQHEDMIDSQIIKAMGAKIIRCRRLDELRVPVITPLMELFRP
                     GKTGVASGKRGIIPHYVAASTFLYDGTIHAARRPVDKIIIRAADYYNVYDTNFIFSDG
                     MELLNGRFVDMRAVYSVLPGRTLKTMNPLSQQPEKKATPQKKPWGKLIAFLFLVTAMI
                     AVARHYFSGDREASAAVPPVPESTVTPAHPLPASTVVSELRQKKPDVLPVSKEWRLAG
                     YVRGASPYFVLLGPAGQVRRYTAFQPWNGSATELNVDGEKVTFWSGSSGTASKGDVLT
                     DKMLSFDVNGK"
     gene            complement(5910..6134)
                     /locus_tag="ACHKFK_004100"
     CDS             complement(5910..6134)
                     /locus_tag="ACHKFK_004100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054325.1"
                     /translation="MHTTGEHAVSHQFHQQATSSPVRVGFCTASNLVDHVSKEHKCPG
                     PVMVPFRFLDHSLTTFGRWVCGERCHHKRN"
     gene            6338..7189
                     /locus_tag="ACHKFK_004101"
     CDS             6338..7189
                     /locus_tag="ACHKFK_004101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054326.1"
                     /translation="MMRLINLLTAFLFLTSACVHASSPAFFVDRIPLNAAIQLAQEDI
                     FRRQYALPPELASDTRPVTLDLSLTGDQKKQREEYVRWLRQLNISVETRNGVDHYRSF
                     KPVAAPEKLVSWVYTPFNRSPAYLAAVLSGTTTGHSSQVSSENAAASSPVTSGSFLSG
                     EGDSLVFRGTRSELARLKELVPLVDVPAQGVVVTGYIYEVQTGRSEGSGLALAAKLCC
                     WVVWQKTRIRRPTQDYHFCQRAGVRSLTRRAARIWLFCYRSKKFECRPGLSGRGISVY
                     PPQRQCA"
     gene            7601..9880
                     /gene="ydeP"
                     /locus_tag="ACHKFK_004102"
     CDS             7601..9880
                     /gene="ydeP"
                     /locus_tag="ACHKFK_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO9054327.1"
                     /translation="MKKKIEFYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDDVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLDRLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTSSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            10128..10325
                     /gene="safA"
                     /locus_tag="ACHKFK_004103"
     CDS             10128..10325
                     /gene="safA"
                     /locus_tag="ACHKFK_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO9054328.1"
                     /translation="MHATIVKNKITQRDNYKGIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            10401..11162
                     /gene="ydeO"
                     /locus_tag="ACHKFK_004104"
     CDS             10401..11162
                     /gene="ydeO"
                     /locus_tag="ACHKFK_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO9054329.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTDNKMSALAI"
BASE COUNT         2729 a         2530 c         2809 g         3217 t
ORIGIN      
        1 aggtggctga ttcggttgtt cgtgactgcc ttgtttctcg ggccatttat agtgatgtac
       61 cagagcatca ccttctcaat gtgatggcct attttcaggc catctccggc tttgatttcg
      121 cccccgttta tgccacttat catgatcgtt acggcgatga gggattgctt aagcgtctga
      181 ctgatttggc ttttgtcacc cgtttcctgc gggttttccg tgaccagcgg gtcaatgagg
      241 tctgtcgcat gctcggcata ctgaaccgca acgcgcctta tatttctgac tggcatcgtg
      301 acctgtttgc tgtccgcagt aaacgcgtga aaaagtatct ccggtcctcc ggtgtctttg
      361 atgcgtttaa tgagctgatc tgtacgctgg aagatgcgca taacgcctct gtttcgaatc
      421 cgaaaaaccg gattgctgaa ctctgcgttc gtggaaaggc cgtctgtgaa ctgtccgaag
      481 atatggggct gtccggctac ttcatcgtgc tcaccacgcc gtcacgtttt cacccgacga
      541 ccagttttaa ggttgccgga aaatggcatt ctcgtccgaa taaaaaatgg tgggaagcag
      601 gttgcccgac cgttaaggat tcgcacgcct ggctgaatac cgtctggcgt cgggtctgcc
      661 gcagactgga taaagccggt attcagatac ccggtctgcg cacggtcgag cctcatgctg
      721 acggtacaac gcactggaat ttcctgattt actgcaatcc ccatgagagc gcaacggtgc
      781 tggccatttt ccgtgaagag gccatgcgcg atgagccgga tgaaaagggg gcgaaagagc
      841 accgtattcg tattgaggct attgaccctg aaaagggtga tggtttccgt tacatcgtga
      901 agtacatcac caaaatggcg ggtgatgtca gtgcggatgg tattaccgcc ctgaatgacc
      961 gttattctgc ccgttcattc tgtgatgccg tcagccgtgc cgcctgctgg cagaaagcaa
     1021 cgcgccttcg cctgttccag ttctttggcg ttccgtccgt cacggcttac cgccagatgc
     1081 gtacttttcg ttcaccgctt gaagcacatc atatcaatat gcagcagttc acgccgcagc
     1141 aggtcgccga actggaagcc atccgcatgg cctgtgatgc aggcgatttc cggacctata
     1201 tcctgctgaa cggcggtttc ttctgttctg aacgtctgct tcgtccgttt tacgttcagc
     1261 cgcaggaagg tggtaagccc cgtattaacc gttacggtga accctgcgca ccggtgattt
     1321 ccggtttcat gtttggcccc gttccggtca taacccgttt tatgggctgc gttgtccgcc
     1381 gtatgacccc cgcagaaaaa atccgcgcag aggaagtcag aagcggcagc agctatgaat
     1441 ctgtgtttat ttcgtcggct tcacgacacc gcacgacgcg ctcccgcgcg gcgggtgggg
     1501 gcggcgaagc cgacccttgg acttgtgaca ataactgtct ctgactgaat ttttaacatt
     1561 gaggagtctg atttatgaat caaatttacg acagttttcc gtttccttcc cctgagggtt
     1621 ttacctcagg catgacaatg gcgggatatt ttattcacgt cactgtcata aatgatgagg
     1681 agttaaaacc cacggattct cattataacc ctgaggctaa acccaaatat gccattgtta
     1741 ttgcctatcc gtatgaggat cgaaaattaa aagtccgccg tgaggagcat gagcgctttt
     1801 cctgtactga ggaggatttt aaatttttta attcatgtcc tgatttgcag ggcaaacctg
     1861 tttatctcac ggtggatgtt aactcatggt cgacaggctc agaacgtcat ggtgtctggt
     1921 atcgttttat ttccggctcc atgaaacgtt ttgacggtca gccgctgggt gcactgcccg
     1981 caggtaaaga aaaaggctga tgatatgtct gacgctgatt ttctgaactt tgttttattt
     2041 gctgcgcctg caatgatttt attttcttca ggttttggca tcggaataaa actgattcgc
     2101 gcctgtgact cacaaagcat tactgttcag cgttattatg actgaatttg atttcagcgg
     2161 tgttttatcc gcactgaatt ctggcgtaca ggacggggag ctttactggt ccgttcttta
     2221 tgtctggatt gtctgctggc ttcttggctt tggtcttggt gttattttca ctctggtctg
     2281 ccggatgttc agtgatgctg ttaattaatt ttaattgtca aagaggaatt gtatgaaact
     2341 gaaaaccctt gttttatctg gtctggcaac gactgcttct gtcgctgcgc ttcctgcatc
     2401 tgccgcagtt caggttccgt cttttattac tgctgatact gttaaagaaa ttggtgacgc
     2461 gctcaccagt gtgattggca ttgcaggtac ggcggcattt gccgttcttg gtgtttcact
     2521 cgctgcccgt ctgggtatcg gtatcattaa aggcttcctt tcccgcgcaa cctgatagtt
     2581 atttaacgac agcgccctcc ggggcgcttt tttaatcata ccaggagtgt tttgtttgat
     2641 tcactttatt attccgcttc tgtttttgtt cttttcctcg ttttctttcg ctgaagagaa
     2701 agaaaatccg gatgcacttg ttcagtgtgc aggccattca tcctgcagga cagattattc
     2761 ggacgcgggg acgtcgatta aattagccag ggcggccggt catcttttct gtggttcggg
     2821 cagttatgac gcatgttccg gaataattca ttattgtggt gtgccacttt ccacgcctgt
     2881 taatgcacct tcgactgtcg ggtcttttgt ccgtgcccgg tggtcagcat ataaagtctg
     2941 ggattcagac aagcaaaagt ggacagcctg cacccttgct tttcagggtg atgaggggga
     3001 tttgacacct gaatataata agccgccaac tgacgagatt tgtctttctc gtccggttct
     3061 tgatggtatg actttttctg atgtttataa aggtgatgac gggacccgtt atgttaatgt
     3121 gcaggggtgt atttatgagg caaccggcaa tcctcttatt tgtaatcccg atgatgatac
     3181 agtttgtact gccgactgga aaccagttgc tgtagatcct gcattctccg atggtaagga
     3241 ggatggcggt agctcaggtg gtggcagttc cggtggtggt aacccaggcg gcggcagttc
     3301 cggtggtggt tcaggttctg gctccggtgg ttcatccggt agcggtggct ccggctcggg
     3361 ttccggcggc tcatccggca gtggtggttc aggttctggc tccggtggtt catccggtag
     3421 cggtggctcc ggctcgggct ccggcggttc atccggtagc ggtagttcag gttcaggatc
     3481 cggtggctca tccggcgatg acaatggctg gcttgatggt attttgggct ggttaaaaac
     3541 aatatgggtg tcagtgaatg atttaaataa tgctttttct gtttctcagg ctgatatgaa
     3601 taatgcatta aattcactgg acaatggtat taaagatatt agtcagaata tagcttcttc
     3661 tttgcctgat ggggatttcc ctttagggga gctttcgcat tatttgccag gacagggaag
     3721 tgagagtcat gataataaca attggcttac agttaatatt gacaaactgg taaatacaaa
     3781 taatggaact gctcttcctt ttactttaca gtttagcttt aatcttccgt tgattggcgc
     3841 gattaatttt aagattgata caagccattt tgcaaaagct tatgacgatt atgttcgttc
     3901 tgttcttgaa tatgctattt atatgataac catgttacgc gtttttgtta tttctcgccg
     3961 tacattattt gaacgtgagg taaaactctg atgtgggcca ttttaggtgc tttttttgtt
     4021 gatgttgttt taaaatcact acgtttcatt gcaggagtta tcggtgataa agctgttgca
     4081 tgggccaagc tcctttctta tcttggtctt tgctacactt catattatgc tcttgtatct
     4141 gccgtcaatt ttattattcg tcaaatctcc gatgttaata aactcgatgt tattgtagtt
     4201 gcattcagtt acctgccttt aaatattcag tactgcttta atatcatcct ccatgttcaa
     4261 tttatttcat acatatatct ttataaggat agagtgtacc gattggttta tgatttattt
     4321 aaatcaaatg ggcttaatgt tggcaagtaa tttttcgggg taagttatgc cagttatcac
     4381 gggtaatctg gggcagggta aaggtattgt cgcagcttat tttgcctcac tttattatcg
     4441 ccgtggatta cgggttgcgg caaattttcc gcttaacact gaatatatgt cctcaggttc
     4501 ggataacccc gttacggtta ttccggctat gccccgcatt gaagactttg agcttcttgg
     4561 tcgtggatgc cctgaaaatg aaaaaacacg cttcggggcg ttatttcttg atgagtgtgc
     4621 gacatggctg aatacccgtg gttttgcccg taaggacaga ttaccgttaa ttgactggct
     4681 gatacattcc cgtaagctgg gctgggatgt ttatctgatt tcacagcatg aagatatgat
     4741 tgattcccag ataattaagg cgatgggggc taaaattatt cgctgccgcc gtcttgatga
     4801 attacgtgtt cctgtgatta cgccactgat ggaattattc agaccaggaa aaacaggggt
     4861 tgcatcaggc aaaaggggga ttattcctca ttacgtggct gccagtacgt ttctttatga
     4921 cggcacgatt cacgcggcac gtcgtcccgt agataaaatc attatcaggg ctgcggatta
     4981 ttacaacgtt tacgacacta atttcatttt ctctgacgga atggagctgc ttaatggccg
     5041 ttttgtggat atgcgtgctg tttattccgt tttgcccggg cgcacactta aaacaatgaa
     5101 tccgttatct caacaacccg aaaaaaaggc caccccacag aaaaagccgt ggggcaagct
     5161 gattgccttt cttttcctcg tcaccgcgat gattgccgtt gcccgacatt atttctccgg
     5221 cgatcgggag gctagtgccg ctgtccctcc tgtgcctgaa agcaccgtga cacctgcaca
     5281 tcctttgcct gcgtctacgg ttgtatcaga gctgagacag aaaaaaccag atgtgcttcc
     5341 ggtatcaaag gagtggcggc ttgccggata tgtcagggga gcaagtcctt attttgtgct
     5401 gctgggtccg gcgggtcagg tgcgacgtta taccgctttt cagccatgga acggcagcgc
     5461 cacggaactg aatgttgacg gtgaaaaagt gacgttctgg tcaggctcat cgggaacggc
     5521 atcaaagggg gatgtgctga ctgacaaaat gctttcgttt gatgtgaatg gtaaataaga
     5581 cagaggacac tccgctcgcc gcagacccag cgaccgaacg tagtgagcga gtggtcgagg
     5641 aagcggaaag gcaccattac cggccccggg cacttacgct ctgctgcctg cttcgggcac
     5701 ttatgctctt tgctgacatg gtcaaccagc ttactggctg tgcagaacac gacgcgtaca
     5761 ggcgatggtg ttgcctgttg atgaaactga tggctgacag cgtgctcccc tgtagtgtgc
     5821 accgcagctt aatccgtgct ttgtttccat ggactgcacc tgcctgccgg actggcaggt
     5881 ctgacggatg aacagagagg cagaatgctt cagttgcgct tgtgatggca ccgctcgccg
     5941 cagacccagc gaccgaacgt agtgagcgag tggtcgagga agcggaaagg caccattacc
     6001 ggccccgggc acttatgctc tttgctgaca tggtcaacca gattactggc tgtgcagaac
     6061 ccgacgcgta caggcgatga tgttgcctgt tgatgaaact gatggctgac agcgtgctcc
     6121 cctgtagtgt gcaccgcagc ttaatccgcg ctttgttttc atggactgca cctgcctgcc
     6181 ggactggcag gtctgatgga tgaacagaca ggcagaatgc ttcatcttcg cgtgtggtgg
     6241 taccgctcgc cgcagatccg gcgaccgaac gcagtgagcg agtgatcgag gaagcggaat
     6301 atcttcatga ctgaaaaaca gcacttaacg gtataacatg atgagattga ttaacttact
     6361 gacagcattt ctttttttga cttcggcgtg tgtgcatgcc tcgtctccgg ccttttttgt
     6421 tgaccgtatt ccgctcaatg cagcgataca gctggctcag gaagatattt tcaggcgtca
     6481 gtatgcgttg ccgcctgaac ttgccagcga tacccgtccg gtgacgctgg acttaagtct
     6541 gacgggcgat cagaaaaaac agcgtgaaga gtatgtgcgc tggctcagac aactgaatat
     6601 ttctgttgag actcgtaatg gcgtggatca ttatcgctcg tttaaacccg tggctgcgcc
     6661 ggaaaaactg gtttcatggg tatatacacc atttaaccgt tcgccagctt atcttgccgc
     6721 cgtgctttcg ggtacaacga cagggcattc atcacaggta tcttcagaga atgcggctgc
     6781 atcttctccg gtaacttccg gctcattctt atcaggtgag ggtgattcac tggttttcag
     6841 ggggacacgt tcagaactgg cacgccttaa ggagctggtt ccgctggttg atgttccggc
     6901 tcagggggtt gtggtaacgg ggtatattta tgaggtgcag acagggcgct ctgaaggttc
     6961 ggggctggca ctggctgcaa aattgtgttg ctgggtggtc tggcagaaaa caaggattcg
     7021 caggccaaca caggattatc atttctgcca aagagctgga gtcagaagtc tgacgagaag
     7081 agccgcacgg atatggttat tctgttacag gtcaaaaaag tttgagtgcc gaccgggatt
     7141 atcaggacgc ggcatctcag tttacccacc acaacgtcag tgcgcataat gcatattatg
     7201 ttaaatcatg tctgagtgat acgaatacgt tgtgatttct gacttccctc ccgtttcggt
     7261 agaaaaattc aacctctgtt cagtgatggc acaccataga ccatgagttg gatacttgcg
     7321 ttgttttgac attagtctat gcgctccatt cacgaaaatt gcgcggataa ttattttgtg
     7381 aaggctatta tcctacactt gtaagaaaat ccgcgcacaa ttgaagccag agtcttattt
     7441 tttatattga gaattatgtt aatttaaacc ctatgagaga taatagtaag agagtagtcc
     7501 cctactctta tatattcatg ttgacgataa accctttgta tatactttgc atcatcgcta
     7561 ttacaaatcc taataattca tttccacaca ggataagcag atgaagaaaa aaattgaatt
     7621 ctaccaggga gctgccggtg gttggggtgc tgttaaatcc gtagcgaatg cagtacgtaa
     7681 gcagatggat atacgccagg atgttattgc catgtttgac atgaataagc cagagggctt
     7741 tgactgtccg ggatgtgcat ggccagatcc taagcacagt gcgtcattcg acatttgtga
     7801 aaacggcgca aaagcaatcg cctgggaagt cacggataag caggtaaatg cctctttctt
     7861 tgctgagaat acggttcaat cattacttac ctggggagat cacgagcttg aggctgcggg
     7921 acgtctcact cagcctttga aatatgatga cgtcagcgac tgttacaagc cattaagctg
     7981 gcaacaagct tttgacgaaa ttggcgctcg ccttcaaagc tatagtgatc ccaatcaggt
     8041 tgaattctat acttcggggc gcacttccaa tgaagctgcc tttctttatc agctttttgc
     8101 ccgtgaatac gggagcaata actttcccga ctgctccaac atgtgccatg aaccgaccag
     8161 tgtgggattg gcagcgagta tcggtgtggg taaagggacc gtgttgctgg aagactttga
     8221 gaaatgcgat ttagtcattt gcattgggca taaccctggt acaaaccacc ctcgcatgct
     8281 gacttcgttg cgcgctttag tgaaacgggg agcgaaaatg atcgctatca accctctaca
     8341 ggaacgtggc ctggagcgat ttaccgcacc gcaaaacccg tttgaaatgc tgacgaactc
     8401 tgagactcag ttggccagtg cctactataa cgtgcgcatt ggtggcgata tggcattgct
     8461 caaggggatg atgcgcctgt taattgagcg cgatgatgct gcaagcgccg caggtcggcc
     8521 ctcattgctg gatgacgaat ttattcaaac gcataccgtc ggctttgacg agctacgccg
     8581 tgacgtgctc aattccgagt ggaaagatat cgaacgtatt tctggactaa gtcagacaca
     8641 aatcgccgaa cttgctgatg catatgccgc tgccgaacga actattatct gttacggaat
     8701 ggggatcact cagcacgaac atggtaccca gaacgtacag caactggtca atctgctgtt
     8761 gatgaaaggt aacattggca agcctggtgc gggtatctgc ccactacgag gtcactctaa
     8821 tgtacagggc gaccgaaccg tcggtatcac cgagaaaccg tctgcagagt ttctggatcg
     8881 cctgggtgag cgctatggct tcaccccacc tcatgcacct ggacatgctg caatcgccag
     8941 catgcaagct atatgtacgg ggcaagctcg agcattgatc tgcatggggg gcaactttgc
     9001 actggcaatg ccagatcggg aagcgagcgc tgtaccgtta acgcaattag atctggcggt
     9061 acacgtagcc actaagctta accgctctca tctgctgacc gcacggcata gctatattct
     9121 gccggttctg ggacgtagcg agattgacat gcaaaaaagc ggtgcgcagg cggtaaccgt
     9181 tgaagattca atgtcgatga ttcatgcctc gcgtggtgtg ttaaaacccg ccggtgtaat
     9241 gctgaaatca gagtgtgcag tggtcgcggg aatcgcgcaa gcagcactac cccagagcgt
     9301 ggtagcctgg gagtatctgg tggaagatta tgatcgcatt cgcaatgaca ttgaagctgt
     9361 gctgccagag tttgccgact ataaccagcg catccgtcat cctggtggtt ttcacctgat
     9421 aaacgcagca gctgaaaggc gctggatgac gtcgtcaggt aaggctaatt tcattaccag
     9481 taaagggctg ttagaagatc cctcttcagc gtttaacagt aagctggtca tggcgacagt
     9541 acgcagtcac gatcagtaca acacgacgat ttatggtatg gatgatcgct atcgaggggt
     9601 attcggtcaa cgagatgtgg tctttatgag tgctaaacaa gctaaaattt gccgtgtaaa
     9661 aaacggcgaa agagttaatc ttattgcact cacgccagac ggtaaacgta gctcacgccg
     9721 catggataga ttaaaagtgg tcatttaccc tatggctgac cgctcactgg tgacctattt
     9781 tccagaatcg aatcacatgc taacacttga taaccacgat ccattaagtg gcattcctgg
     9841 ctataaaagt attccggttg aattagaacc atcaaattaa ttcgcctcct catttcttct
     9901 gctgtcatcc gcacagcaga agaattcctc attgactatt atcgcgcaat ttgctcacat
     9961 ggattaaatt aaaccacaca ctataagata taaacttctg cctacagctg taagaaactc
    10021 cgctcagtac tgaagtaccc gtcctatttc ctctattctc cagcctgtta tattaagcat
    10081 actgattaac gatgcttaac gtcattcgtt taataaacac atttgaaatg catgcgacca
    10141 tagtgaaaaa taaaatcacg caaagagaca actataaagg gatcatgtct gtaattgtgg
    10201 ttgtcttatt actgaccctt acattgatag ccattttttc ggcaattgat cagctgggta
    10261 tttcagaaat gggtcgcatt gcaagggatc ttacacattt cattatcaat agtttgcaag
    10321 actgaaaaac aaacagcaaa ttataaatat gaaatgttaa aaaagtatcg ataaaaactt
    10381 tattgtttta aggagataaa atgtcgctcg tttgttctgt tatatttatt catcatgcct
    10441 tcaacgctaa cattttagat aaagattacg ccttctctga cggcgagatc ctaatggtag
    10501 ataacgctgt tcgtacgcat tttgaacctt atgagcggca tttcaaagaa atcggattta
    10561 atgaaaatac cattaaaaaa tatctacaat gcactaacat ccagacagtg acgatgcctg
    10621 ttcctgcgaa gtttttacgt gcttcaaatg taccgactgg attgcttaat gaaatgattg
    10681 cttatctcaa ctcggaagaa cgcaatcatc ataatttttc agaacttttg cttttttctt
    10741 gcctgtctat ttttgccgca tgcaaaggtt tcattacact attaactaac ggtgtgctat
    10801 ccgtttccgg gaaagtgaga aatattgtca acatgaagct ggcgcaccca tggaagctga
    10861 aagatatttg tgactgcctg tacatcagtg aaagcctgtt gaagaaaaaa ctaaagcaag
    10921 agcaaacgac attctcacag attcttttag atgccagaat gcagcacgca aaaaatttga
    10981 tacgcgtaga aggttcagtc aataaaattg ccgaacaatg tggttatgcg agtacatctt
    11041 attttattta tgcgttccgc aaacatttcg gcaacagtcc aaagagagtt tctaaggagt
    11101 accgttgtca aaggcacacg ggtatgaaca cggataacaa gatgagtgct ttagctattt
    11161 gattatttgc taacgagtag tcaaccacac acgctgcgta agaattaaat ggggcagcca
    11221 ttccctgccc cgcgttgttt ttaggtgata tatttcttga aataaataag tgacagcctt
    11281 cacat
//
LOCUS       DAZGJT010000127        10990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZGJT010000127 DAZGJT010000000
VERSION     DAZGJT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.127"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..410)
                     /gene="chbB"
                     /locus_tag="ACHKFK_004105"
     CDS             complement(90..410)
                     /gene="chbB"
                     /locus_tag="ACHKFK_004105"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO9054330.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(709..1047)
                     /gene="osmE"
                     /locus_tag="ACHKFK_004106"
     CDS             complement(709..1047)
                     /gene="osmE"
                     /locus_tag="ACHKFK_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO9054331.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQATK"
     gene            1249..2076
                     /gene="nadE"
                     /locus_tag="ACHKFK_004107"
     CDS             1249..2076
                     /gene="nadE"
                     /locus_tag="ACHKFK_004107"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO9054332.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2306..3193
                     /gene="cho"
                     /locus_tag="ACHKFK_004108"
     CDS             2306..3193
                     /gene="cho"
                     /locus_tag="ACHKFK_004108"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO9054333.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSINIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLSEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHDDHALRLRQSLERLRVVCW
                     PWKGAVALKEQHPEMTQYHIIQNWLWLGAVNSLKEATTLIRAPAGFDHDGYKILCKPL
                     LSGNYEITELDPVNDQQAS"
     gene            complement(3153..3728)
                     /gene="ves"
                     /locus_tag="ACHKFK_004109"
     CDS             complement(3153..3728)
                     /gene="ves"
                     /locus_tag="ACHKFK_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO9054334.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASITSIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFSFAADLVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3931..4416)
                     /gene="spy"
                     /locus_tag="ACHKFK_004110"
     CDS             complement(3931..4416)
                     /gene="spy"
                     /locus_tag="ACHKFK_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO9054335.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHEDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4746..5714)
                     /gene="astE"
                     /locus_tag="ACHKFK_004111"
     CDS             complement(4746..5714)
                     /gene="astE"
                     /locus_tag="ACHKFK_004111"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO9054336.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQDPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMANDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5707..7050)
                     /gene="astB"
                     /locus_tag="ACHKFK_004112"
     CDS             complement(5707..7050)
                     /gene="astB"
                     /locus_tag="ACHKFK_004112"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO9054337.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHCFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGIQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPVVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRSRVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWRY
                     LNELLAADNPISSLKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDTLTQLLDLGSVYPFQREGGGNG"
     gene            complement(7047..8525)
                     /gene="astD"
                     /locus_tag="ACHKFK_004113"
     CDS             complement(7047..8525)
                     /gene="astD"
                     /locus_tag="ACHKFK_004113"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO9054338.1"
                     /translation="MTLWINGDWVTGQGALRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSFGDRQVRVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGDAVMRLWQQAGLPPGVLNLVQGGRATGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLFLKSGTQGDAFLARLVAVSQRLTPGTWDDEPQPFIGGLISEQAAQQVVTAW
                     QELEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVTGLPDEEVFGPLLRVWRYDTFDEA
                     IRMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8522..9556)
                     /gene="astA"
                     /locus_tag="ACHKFK_004114"
     CDS             complement(8522..9556)
                     /gene="astA"
                     /locus_tag="ACHKFK_004114"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO9054339.1"
                     /translation="MMVIRPVERSDVSALMLLASKTGGGLTSLPANEATLSVRIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPEWRKEGNGYLLSKSRFMFMAAFRDKFNDKAV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYCNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLVRTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9553..10773)
                     /gene="astC"
                     /locus_tag="ACHKFK_004115"
     CDS             complement(9553..10773)
                     /gene="astC"
                     /locus_tag="ACHKFK_004115"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO9054340.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSDIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASAQIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECASVMTVG
                     THGTTYGGNPLASAVAGKVLDLINTPEMLNGVKQRHDWFVERLNSINHHYSLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAVKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FASACEHFVSRGSS"
BASE COUNT         2705 a         2996 c         2684 g         2605 t
ORIGIN      
        1 ctcatagaaa aatacccttt aaaaccgcaa tttaaatatt gcggtattga tttatgaaat
       61 aactctttga cgggaaaatt taaaataaat taatttgctg cggctttttt aatcgctgca
      121 accgcagcct taagcacgcc taaaccatcg actttgccat aaagcagcga gtcaattact
      181 tcaaccggtt tgttgggtaa caaacgctgg atttcgggca acatataagc aatctgcggc
      241 cctaataaca cgacatcggc attctgacct ttttcaccag ccagtgtttc cggaaatgct
      301 tcaataatga ccggaacttc atatttttct gcctgtgcgc gcatttttga taccagtaaa
      361 gaggtagaca tgcccgcaga acaaaacaga taaatgtgtt tcttttccat aaaactgccc
      421 tcatcgacga ttatctgtca gccagacact ccgcaagcct taacctgctt ccatgctctg
      481 ggtaacttgc gaaaccaaca tgactttttt tgtggctgaa aggagtatac ggtataggcc
      541 tgagcaatag tatttctttg accttataaa ttgtctctcc ttgacctgtg gttatgaccc
      601 tcttcacatt tcgggcaaat aattcgcgaa gataattaag aacacaggcc aaaaaaaacc
      661 ggcgcaatgg ccggtttccg ttgttactca agctttcagg cgaagtgatt acttcgtagc
      721 ctgtggatca gtgtcgtatt cagcacaggt ctgataaccg gagttgatga catgtccggt
      781 atcatctaac gcgacaaagt aggtttctgc tttaccatca cgttgaccca ggatgtaggt
      841 ctggcaagta ccacgagcat ggatcatgct cacttcagac gaaggtttac ccgcaatttg
      901 tgcaacctgc gcccggctca tgcctttttt gacgtctttc accacaggct gtacaaactg
      961 gtctttggta cgatcataag ccgtacaacc cgccagcatg gttaataccg ccgctgcact
     1021 cagaattcct gccatattct tgttcatatt ccgtcctctt gtttatcagc gtgttagata
     1081 agcctggaat acattgggcg ctttttcaag cccgtgaacg aaacggctcc gctttcagag
     1141 gattcctgta tgacgtttta accaccattc agcccgctgt cgcttgtcgt ttcagtagca
     1201 acgggttagc tttaaggaag ttttgtcttt tctgtctgga ggggttcaat gacattgcaa
     1261 caacaaataa taaaggcgct gggcgcaaaa ccgcagatta atgctgaaga ggaaattcgt
     1321 cgtagtatcg attttctgaa aagctacctg caaacttatc cgttcattaa atcactggtg
     1381 ctcgggatca gcggcggtca ggattccacg cttgccggta agctgtgcca gatggcgatt
     1441 aatgagctgc gccaggaaac cggcaacgaa tcactgcaat ttattgccgt acgcctgccc
     1501 tatggtgttc aggccgacga acaagattgc caggatgcca tcgcctttat tcaaccggat
     1561 cgcgtattaa ccgttaatat caagggcgcg gtactcgcca gcgagcaggc gctgcgcgaa
     1621 gcgggcattg aactgagcga ttttgtccgt ggcaatgaaa aagcgcgtga gcggatgaaa
     1681 gcacaatata gcattgcggg tatgaccagc ggtgtcgtgg tgggcaccga tcatgcagca
     1741 gaagccatta ccggattctt cactaaatat ggtgacggcg gtacggacat taatccgctg
     1801 tatcgtctca acaaacgtca gggtaaacag ttactggcgg cattaggttg cccggaacac
     1861 ctttataaga aagcgccaac ggccgatctt gaagatgatc gcccttctct accagatgaa
     1921 gtggcactcg gcgtgaccta tgacaatatc gacgactatc tggaagggaa aaacgtacct
     1981 gaacaggtcg ccagaacaat agagaactgg tatctgaaaa ccgaacataa acgccgtccg
     2041 ccaattaccg ttttcgatga tttctggaaa aagtaataat ttgcacatat tggtttgtgc
     2101 gaaaaagagt actttgttca cgccggatgc ggcgtgaacg ccttacccga cctacaaaac
     2161 tatgcaaatt caatacattg caggagtcga ataggcctga caggcgtagc acgtcagacg
     2221 gtataacctt tgtcatcgcc ccgcctcttt tttaatcgct ccccgcctgt tacactggat
     2281 agataaccag cactcggagt caacagtggt acggcgttta acttctccgc ggctcgaatt
     2341 tgaagctgcg gcaatttatg aatatcccga acatttacgt tcattcctta atgacttacc
     2401 cacccgacca ggggtgtatc tgtttcatgg cgaaagtgac accatgccgc tctatatcgg
     2461 caaaagcatt aacatccgca gccgcgtcct ttctcattta cgtaccccgg atgaagccgc
     2521 catgctacgg caatcccgac gcatcagctg gatatgtacc gccggtgaaa tcggcgctct
     2581 gctccttgaa gcgcgattaa tcaaagaaca acagccactg tttaataaac gcttgcggcg
     2641 caatcgccaa ctctgtgccc tgcaattaag tgaaaagcgc gtcgatgtgg tgtatgccaa
     2701 agaggtggat ttttcacgag cccccaacct gtttggcctg tttgccaata ggcgcgcagc
     2761 tttgcaagca ttgcagacca tcgctgatga acaaaaactt tgttatggcc tgttgggact
     2821 ggagccgtta agtcgcggtc gtgcatgttt tcgttcagcg ctaaaacgtt gcgccggagc
     2881 atgctgcggt aaagagagcc atgacgatca tgcgctacgt ttgcgccagt ctctggaacg
     2941 tttacgagtc gtttgttggc cgtggaaagg tgcggtagcg ctgaaagaac agcacccgga
     3001 aatgactcaa tatcatatta ttcaaaactg gctgtggctg ggggcggtta attcgctgaa
     3061 agaagcgaca acgttaattc gggcacccgc cgggtttgat cacgacggtt ataaaattct
     3121 ttgtaagccg ctgctttccg gtaactatga aattactgaa cttgatccgg tgaatgacca
     3181 gcaagccagt tgatttcact gaacaacagt ttaccttgcg gttgcaaaag ccgcagggtg
     3241 tgcctgccat cgaaccagca cgcgccctgg tcggtagtga gcagtttgtc gcccaattgc
     3301 cacgccccgt ttattacaaa caccaccccg ccgcgtgaac caaaggtagt aaacgtacgt
     3361 tcggcaattc tgactttcgc cttacagaca tccagtcgtg tcatgatgtt gaaatccatc
     3421 gacatctgcc ctgccgtcag tttcgctttt accaccaggt ccgctgcaaa ggaaaaaggt
     3481 tgtaacggtt ttaaggtatg gttaaagcgg tctgcgcttt caaggaacat ctcgccgcct
     3541 tccagcaacg tcactatcct ttccataccg ggaaacaaag aaaactcccc attcgccgcg
     3601 atggatgtaa tgctggcacg ccagtaaaaa tcacgttttg ccggtgggaa agtgcaaatt
     3661 tcgcgcgttt caccggcagc gtttcgccac agattcaccg acattttacg catatcaaag
     3721 tattccatac ccgctcccgg cccgttcttt tcattgttat cgttcaggta accgtggatg
     3781 gttatcggca actcgggtga tttacgttag tggcgatcag gctttttttg catctgtttg
     3841 agaagagaaa agaaaaccgc cgatcctgtc caccgcatta ctgcaaggta gtggacaaga
     3901 ccggcggtct tacgtttttt ggctgaaaga ttattcagca gttgcaggca ttttaccttt
     3961 tgccgctgga cgttctgtca gacgcttctc aaaattagca ttaaattgct ttttctgttc
     4021 cggcgtcagg atgttgtaaa tcttgttctg ggtttccatg tgcgccagca tgttagcttt
     4081 gcgctgttct tccatttttg cgatctgcgc ttcagctttt gctttatcga aggtatcgct
     4141 ggcaatgatg tcatgcattg cgcggcgttc ttccagcggc ggacgtttca tctggtcacg
     4201 ctggcctttc atgatttcgc ggatctgctg tttctgcgcg tcggtcaggt tcaggtcttt
     4261 gaacatcatg tcctcatgcg gaccgaactt gcctttatgg tgcatcatcg gcttcgcgtc
     4321 agccggtgct gcggtagtgg tgtctgcggc atgggccagg ttagccgcgc caagagccag
     4381 ggtagaggca acaaacagtg cagttaattt acgcatattc tatatccttc ctttcagtta
     4441 tttattacgg ctttcttaag tagcgtgccg tgttgacgag attaacttta ctgactttag
     4501 cgtcaattaa tcagagcaac ggtaaaacaa tgaaagtgta aaaaacactt ttgcgccaat
     4561 tatggagaaa aaaagaaaat ttgatggaga gtgatgagag aatattacaa cacgatgatt
     4621 ttgcagagat tatgaagaac tataccggat gactggtgat aaataaagca aataaccagg
     4681 attaatctgt attaatttat aagaaagcaa cttaataccc gcagaatgat ttctgcgggt
     4741 aagtattagc ttattttttc gagcattaat cccgcacgta atcccaacgc taccaacgga
     4801 ttagggaata acacatactc tacatcatgg gttacggtaa aacgttcctc cccgtcctgc
     4861 gccagcaatg ttcctttctc aaacggcata aaattcagcg tgtcatttgc catatgcatt
     4921 tcgaaggacg gcgagtggcg agtaatttgc gaaaccaccc gataacggag cggcggtgtt
     4981 ctcacgatac cgacactctc accagatagc agcgcagcaa ttgcgctggc agttactgca
     5041 aactggcgaa gatcgttttg cccaaagggc aacgctttgc caagttccag cgtacaggcc
     5101 agcgcgccaa aatgtctggc gctgaaatgg gtaaacgtac caccagggtc ctgatggaac
     5161 accagcgcct ccagccccgc cgcacccagc cacgtcagaa atttctcgtc ccagggaatg
     5221 tcgcgttgcg gtaatacacc gaactgcgga tgcaaggagc cacgaattgc ggtatgtaga
     5281 tcaaggtgcc agcgcacaga ttctttgccc tggtcataaa aatcttccag gcactgctcc
     5341 agttcgcgcg cccgacaggt ttccccgctt tcagcaaaca gttgccaacg accgccaaac
     5401 attcgattca tatcgctatg gcaataacgt ttcccttgct tcagcgcagg aggattcccg
     5461 aggatcacca gtaaccgcca acgtaacggg atctcaccgt gagatatcgc gccaagcaac
     5521 gcgtccagca tttccacagg agccgtctca ttaccgtgga tccccgctga aatcaccagc
     5581 gcgccttgcg gtggcgttaa tggcgtcagt tccagcacac catcgcccag ccagcgccag
     5641 cgaacgccgt tgatttctcg ctcggtgata accggttttt tacccgttaa ggttagagca
     5701 agaaaattat ccattgcccc ctccctcgcg ctggaatgga taaaccgaac caagatccaa
     5761 taattgagtc agtgtatcca gcgcttccct tccttcgcgc agcaattgtg gatcggcaag
     5821 atccgccgca gtaagacgat cgcggtagta acgatccacc cagtcattga gcgcattaaa
     5881 cagcgtatcg ttcatcatca ccgccggatt caccgcccgg cgttcttctt ctgtcaacac
     5941 cacgcgcaac cgcaggcacg ccgggccacc gccattcgcc atgctttcac gtaaatcaaa
     6001 aacttttagt gagcttattg ggttatcggc agcaagcaat tcattgagat aacgccatac
     6061 tccagcatgt tcccgacatt cctgaggcag caccaacatc atggaaccat catcgcggct
     6121 cagtaactgg ctgttaaaca gatacgtaga caccgcatcc gacacggaaa cctgagtcgc
     6181 cggaacttct atcgccataa aaccattgac ccgcgaacgc aggtttgcca gtaactgtga
     6241 ctggcgagcg aacgcctgtt gatggcaaaa cagcacctgg cggttactca cggcaatcac
     6301 gtcattatga aaaacgccct ggtcgataac gaccgggttt tgctgggcga aaatcacctg
     6361 ttggggattc acctgattca accttgccac cgcctcgctg gcttcgcgag tctgccgcgc
     6421 cggataacgg gaaggccggg tatcattgcc ctcttctcgc ccgtagacaa aaagttgaat
     6481 acccggttca ccgtaatgac cgccgagacg attgtggttt gccgctccct catcaccgag
     6541 caaggctacc tgcggcaacg ccgaatggac actaaatttc tcttcgtcgt taaaaatcgc
     6601 ttttaacaac gattcggtga cgggggcttc cagcgaacgg tgaaatttat tgttcaggtt
     6661 ggcaacggtg agatgcactt tgccatccag cgtatcggca gatggcgcga tcgttgccgc
     6721 attggctacc cacattggcg aagcggagct gacgctggaa agccagtgcg gtgcctggcg
     6781 ggcaaccttt tccagcactt gttcatcgct accactgaat cccaactgac gtaaaaccgg
     6841 tataaacgga cgctcgtgcg gcgggatcac ggcctggggg aatcccgcat cggcaagggt
     6901 tttcattttc agtaagccct gtttcgccgc cagtcgcggg ttagagacct gaaaacaatg
     6961 acgggtagag gcttcattac caaacgacag gcccgcgtaa tgatgcgtca gccctaccag
     7021 cccgtcgaaa ttgacttccc aggcgttcat cgcaccacct catcggaaaa atccagccca
     7081 gggttaagcg tagcgggcaa tgttaacgag tccgactcca ggctcgccat cggccaggca
     7141 caataatctg cggcatacca ggcgctgggg cgatggttac cggaagcgcc aatgccgccg
     7201 aatggcgcgg tactggcagc gccggtaagc ggtttgttcc agttaacaat ccccgcccgc
     7261 gcctccagca acagttgatc gaatttttcc cgctcggggg aaaccagacc gcaagagagg
     7321 ccaaagcgag tgttattcgc cattcgaatc gcttcatcga aagtatcata acgccagacg
     7381 cgcaataacg gtccgaacac ttcttcatcc ggcaagccag taacgcctgt catttcaatg
     7441 atccccggcg tcagcaacga tgtccctgct tgtaataagc gcggcgcaag cagggtccgt
     7501 ccgcccatcg cctccagttc ctgccaggca gtaaccacct gctgtgcggc ctgctcagaa
     7561 atcagcccac caataaatgg ctgcggttcg tcatcccaag tgcccggcgt taaccgctgg
     7621 ctgacggcaa ccaggcgagc aagaaatgca tcgccctgcg ttccactttt cagaaataaa
     7681 cggcgggcgc aggtgcagcg ttgcccggca gtgacaaacg ccgactgaat ggtcagatgg
     7741 acagcggcat cgatatccgc cacctcatcg ataattagcg ggttattacc gcccatctca
     7801 agggcgagaa ttttctccgg ctgaccggaa agctggcgat gcagctggta gccggtattg
     7861 gcgctgccgg taaacagcaa gccgtcgaga tcctccagcg cactcagcgc ctgacccgtt
     7921 gcacgcccgc cctgcaccag gttcagcacg cccggcggca agccagcctg ctgccataaa
     7981 cgcattaccg cgtcgccact ccacggtgtc agttcgctgg gtttaaagat aatggtgtta
     8041 cctgccagca atgccggaac gatatgtccg ttcggcaaat gaccagggaa attatacggc
     8101 ccaaacaccg ccagcacgcc gtgcgggcga tgtcgcaggc tcgccgcgcc gtccggcatt
     8161 tcgctacgct gctcaccggt acgaacgtga tacgccttaa ttgatatcgc gattttattg
     8221 atcatcgccg tcacttcggt tgccgcttcc cagcgcggct tgccggtttc tctggcaata
     8281 atcgcggtta attcggcttt attgctttcc agcaatccgg caaagcgttc aactcttacc
     8341 tgacgatcgc caaatgagag ccgagcccag cgcggaaacg ccgcacgggc agcccgacaa
     8401 gcctgcccga cctgagcggc atcggcatca ttgccttgcc ataacacctc gcccgatacc
     8461 ggattacgct tcacacgcaa tgcgccctgg cccgttaccc agtcaccgtt gatccataaa
     8521 gtcatgctgt tttctcctct gcgcacaggc gcaccagacg aacgcgatcc cctgcgtggc
     8581 atttgagggc atccagttgt gcggcggtta aaatcaaacg ctcggtcgct ggatcggtac
     8641 gcaccagcac cacgcggaaa tggtgataat tttcattggc gaccaggcag gccggaaaat
     8701 cgccctgcgc aggctgccct tcggccactt ccaccagccg acttttacgg atggcgcgca
     8761 cgcggtcgat atcacattca agtgtcggcc cgccgtcaaa gatgtcgata tagttacagt
     8821 agcgaaaacc ttctttctcc agcaccgcac gggcaggcgc ggtttgcgga tgtacctgac
     8881 cgatgacgtc ctgggcttcc tgggataaaa agtgggtata gatcggatgt ttcggcatca
     8941 gttctgcaat aaatgccttt tgcccggtgc cgcagagaaa atcagcgcgg ctaaaatcca
     9001 tcgaaaagaa gcgtttaccg aggctttgcc agaacggtga atagccgtgt tcgtcaatca
     9061 ccccgcgcat ttcggcgacc gctttgtcat taaacttgtc gcgaaaagcc gccataaaca
     9121 taaagcgcga tttcgacagc aaatagccgt tgccctcttt gcgccactcc gggtcgagaa
     9181 acagcgtgca cagctcgctg ctgccggtgt gatcgttact gagaaacagc gtcggcaatg
     9241 cgttatagac attcagctct ttcgaagcgt gaaccagcgt gccgacacga tagttgtacc
     9301 agggatcgtt cagcccaacc gccacctcaa tggcgcaaat ccccgccaca gtgcctgtct
     9361 cactatcttc cagtacaaac acataaccct gctcactttt gggcagttcg ccttgccagg
     9421 ttttgattgc cctttcaata cgcaccgaaa gcgtggcttc attggcggga agcgacgtca
     9481 ggccgccgcc cgttttgctg gcaagcagca tcagcgccga gacatctgag cgctcaacgg
     9541 gacgaatgac catcatgatg aacctcggct aacaaagtgt tcgcaagcag atgcaaagcg
     9601 atccagtccg gtcgtcacct cttcttcgct gacattgagc gcaggcgcaa agcgcaccac
     9661 gttgccaccc gcaatcagca ccatcacgcc tgctttcacc gcttcctgag agatctgttt
     9721 cgcttgcccg gcgtaatcgg cattcagtac acagccaatc aaaagaccta agccgcgaac
     9781 ttcactgaac aaactgtagt ggtgattaat ggaatttaga cgctcaacaa accagtcgtg
     9841 acgctgttta accccgttaa gcatctctgg cgtgttgatg agatccagca ctttgcctgc
     9901 caccgccgag gccagcggat tgccgccata ggtggtgccg tgagtgccaa cggtcatcac
     9961 gctggcgcac tcttcggttg ccaacaacgc accaaccggg aaaccgccgc ccagcgcttt
    10021 ggcggtagtt aacagatcag gcgtcacgcc atagtgcata taggcataca gttccccggt
    10081 gcgcccgaca ccggtttgta cttcatcaaa gatcagcaac gcattgtggc ggtcacacaa
    10141 ttcacgcaga ccttgtaaaa atgcgttgct ggctggcacc acaccgcctt ccccctggat
    10201 gggttcgaca atcaccgcac aggtggcgtc gtcaatctgc gcactggcag agttaatatc
    10261 gttatatgca gcatgacgaa tatccggcgg cagtggcgca aaatcctgtg aataggctgg
    10321 ctgcccaccc gcactgacag taaacagcgt gcgaccatga aacgcatttt tgaacgccac
    10381 gatgtcgctc ttatggctgc cgtagcggtc gtgagcgaat ttacgcgcca gttttagcgc
    10441 cgcttcgttg gcttccgcac cggagttaca aaagaagacg cgatcggcaa acgtggcgtc
    10501 gatcaatttt ttcgccagtc gcagtaccgg ctcgttggtg taaccgttgc cggtatgcca
    10561 gaacttactc gcctgttcgt tcagcgcctc acgcagttcc ggatgcgcat ggcccagcgc
    10621 gttcaccgca atgccacccg cgaagtcgat atactctttc ccctgctgat cccacaagcg
    10681 cgaaccttcg ccacgtaccg gtataaacgg tgccggagcg taaacaggta tcatccattc
    10741 atcaaagttt tcacgcgtaa ttggctgaga catagcgacc tctacagtaa ataattcgtt
    10801 attcatatgt taataataag taatgtttgc gctgtaaatg tagattgcag ggttcgtgcc
    10861 agccagtgat aaaagtgcat aaacggcgga ggctaacttg aaatcaagca gttataacca
    10921 catcatatgc atttaaagtg catataaagt gaatacgttt gcgatgtggg tgaataaaaa
    10981 gaataaaaaa
//
LOCUS       DAZGJT010000128        10967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZGJT010000128 DAZGJT010000000
VERSION     DAZGJT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.128"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..833
                     /gene="adiY"
                     /locus_tag="ACHKFK_004116"
     CDS             72..833
                     /gene="adiY"
                     /locus_tag="ACHKFK_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO9054341.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            970..2307
                     /gene="adiC"
                     /locus_tag="ACHKFK_004117"
     CDS             970..2307
                     /gene="adiC"
                     /locus_tag="ACHKFK_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO9054342.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2411..4054
                     /gene="eptA"
                     /locus_tag="ACHKFK_004118"
     CDS             2411..4054
                     /gene="eptA"
                     /locus_tag="ACHKFK_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO9054343.1"
                     /translation="MLKRLLKRPSLNLFSWLLLATFYISVCLNIAFFKQVLQVLPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLGSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMITNIIDTTPAESYALMTPRMLLTLGLSGVLAAIIACWIKITPTTSRLRSVLFRGAN
                     ILISVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRSANLPLIR
                     IGEDAHRNPLMQNGKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGVCD
                     RVPHQNITALNLPGQCINGECYDEVLFHGLEDYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAANDILQTCRRVSE"
     gene            4051..4719
                     /gene="basR"
                     /locus_tag="ACHKFK_004119"
     CDS             4051..4719
                     /gene="basR"
                     /locus_tag="ACHKFK_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO9054344.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVSTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELNVGNLTLNMGRRQVWMSGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4720..5820
                     /gene="pmrB"
                     /locus_tag="ACHKFK_004120"
     CDS             4720..5820
                     /gene="pmrB"
                     /locus_tag="ACHKFK_004120"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO9054345.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIDAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMENVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWIRLKKDQYVANQI"
     gene            complement(5796..5885)
                     /gene="pmrR"
                     /locus_tag="ACHKFK_004121"
     CDS             complement(5796..5885)
                     /gene="pmrR"
                     /locus_tag="ACHKFK_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO9054346.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5997..7499)
                     /gene="proP"
                     /locus_tag="ACHKFK_004122"
     CDS             complement(5997..7499)
                     /gene="proP"
                     /locus_tag="ACHKFK_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO9054347.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEENFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVVGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7763..8641)
                     /gene="rdcB"
                     /locus_tag="ACHKFK_004123"
     CDS             complement(7763..8641)
                     /gene="rdcB"
                     /locus_tag="ACHKFK_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO9054348.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKQASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIVDLYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGRPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVSTGGEHADFLSWLGKWSTADKQPVCWSVTQRWRTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLV"
     gene            complement(8638..10866)
                     /gene="crfC"
                     /locus_tag="ACHKFK_004124"
     CDS             complement(8638..10866)
                     /gene="crfC"
                     /locus_tag="ACHKFK_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO9054349.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISQLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDHLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGIAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLYAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LQQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSTNHFLRQQQDAVNAQLAALFQD
                     DSESLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQAATDEQITRFGVVREIFSRWLNQPD
                     WGRGNEKFPTETVDYSVLQRALSAEVDLYCQQMAKVMAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2767 a         2875 c         2662 g         2663 t
ORIGIN      
        1 atataaccat ttcccggtat cgcttcgctt tagcgagaac tggtctttta ttcgcactca
       61 ggagtacatg tatgaggatt tgcagcgacc aaccttgtat tgttttattg actgaaaaag
      121 atgtctggat aagggtgaat gggaaagaac ctattagcct taaagctaac cacatggcgt
      181 tattaaattg tgaaaataat attatcgacg tctcctctct taataacact ttggttgctc
      241 atattagtca cgacatcatc aaagattacc tccggtttct gaataaagat ctctcgcaaa
      301 taccagtatg gcaacgtagc gctacgccca tcctcaccct gccatgcctg acgccagacg
      361 tctttcgcgt tgccgcgcaa catagcatga tgcccgctga aactgagtca gaaaaggaac
      421 gaacacgtgc attattattc actgtgctat cccgttttct cgacagtaaa aaattccttt
      481 cactaatgat gtatatgtta cgtaattgtg taagtgacag cgtttatcaa attattgaaa
      541 gcgatatcca caaagactgg aatcttagta tggtagccag ttgtttatgt cttagcccaa
      601 gtctgttaaa gaaaaagctg aaaagcgaaa acaccagtta tagccaaata atcaccacct
      661 gccgcatgcg ttatgccgta aatgaattaa tgatggacgg taaaaatatc tctcaggtat
      721 cacagtcctg cggctacaac agtacgtcgt actttatttc tgtctttaaa gacttctacg
      781 gtatgacgcc gctgcattat gttagtcagc acagagaacg cactgtcgcc tgatttttaa
      841 ccttaacgaa gagctatatt aataacggca tcagcgataa cccagccgat aataattcaa
      901 ctatcgaatg caggcgtatg atatgacgta atttattgtc acgaagctcg ccttcgcagg
      961 agtttaatta tgtcttcgga tgctgatgct cacaaagtgg gcttaatccc cgtcaccctg
     1021 atggtgtcgg ggaatattat ggggtcaggt gtttttctgt tacctgcaaa cctggcctcc
     1081 actggcggga ttgccattta tggatggttg gtgacgatta tcggtgcgct ggggctctcg
     1141 atggtatacg ccaaaatgtc gttcctcgac ccaagtcctg gtggttctta cgcttacgcc
     1201 cgccgctgct ttggcccgtt tctcggttat caaaccaacg tcctctactg gctggcctgc
     1261 tggatcggca atatcgccat ggtggtcatt ggcgtaggat atttaagtta cttcttcccg
     1321 attctgaaag acccattggt attaaccatc acctgcgtcg tggtgctgtg gatcttcgtc
     1381 ctgctgaaca ttgtcggtcc gaaaatgatc acccgcgtac aggccgttgc taccgtgctg
     1441 gcgctgatcc ccatcgtcgg gattgccgta tttggctggt tctggttccg tggtgaaacc
     1501 tatatggcgg catggaacgt cagcggtctg ggcaccttcg gtgcgattca aagtaccctt
     1561 aacgttacgc tgtggtcgtt catcggtgtg gaaagtgcct ccgttgccgc aggtgtggtg
     1621 aaaaacccga aacgcaacgt ccctatcgcc accattggcg gggtattgat tgctgccgtt
     1681 tgctatgtac tttctaccac cgcgattatg gggatgatcc ctaatgccgc actgcgcgtt
     1741 tctgcttcgc cattcggtga tgccgcacgg atggcgttgg gtgacaccgc cggggccatt
     1801 gtttccttct gcgcagctgc gggttgctta ggttcactgg gcggctggac gttgctggcg
     1861 ggtcaaacgg cgaaagccgc tgccgatgac ggactgttcc caccgatttt tgcccgtgta
     1921 aataaagcgg gcacaccagt ggcggggctg attatcgtcg gtattttgat gaccatcttc
     1981 cagctcagta gcatttcgcc aaacgcgacc aaagaattcg gtctggtttc ttccgtgtcg
     2041 gtcatcttta cactggtgcc atatctttac acctgtgcag cgttactgct gctcggccac
     2101 ggtcactttg gtaaagcgcg cccggcatac ctggcagtta ctaccattgc cttcctctac
     2161 tgcatctggg ccgtagtagg gtccggcgcg aaagaggtta tgtggtcatt tgtcactctg
     2221 atggtaatca ccgccatgta tgcgctgaat tacaaccggc tgcataaaaa cccgtatccc
     2281 ttagatgcac caataagcaa agattaattc cccttaatca agcaaacata aaagccaacc
     2341 ttaagaactt aaggttggct taattttgct ttgcgagcat atgcgcactt tgttcgatgg
     2401 aaacaccgtg atgttgaagc gcctactaaa aagaccctct ttaaatttat tctcctggtt
     2461 attacttgct actttttata tctcagtctg cctgaatatc gcctttttta aacaggtact
     2521 acaggtgtta ccgctggatt cactgcataa cgtgctggtt ttcttgtcga tgccggtcgt
     2581 cgcattcagc gtgattaata ttgtcctgac actaggctct ttcttatggc ttaatcgtcc
     2641 actcgcctgc ctgtttattc tggtcggtgc ggctgcacaa tatttcatca tgacctatgg
     2701 catcgtcatc gaccgttcaa tgatcaccaa tatcattgat actacgccgg cggaaagtta
     2761 tgccctgatg acgccacgaa tgttattaac gctggggtta agtggcgtgt tagccgcgat
     2821 tattgcctgc tggatcaaaa tcacccccac cacctcacgc ctgcgcagcg ttcttttccg
     2881 cggggccaac atcctgattt ctgtactgct gattctgctg gttgccgcac tgttctacaa
     2941 agactatgcc tcgctgtttc gcaacaacaa agagctggtg aaatccttaa gcccgtccaa
     3001 cagcattgtt gctagctggt cgtggtactc tcaccagcgg tcagccaacc tgccactgat
     3061 acgcattggt gaagatgctc accgcaaccc attaatgcaa aatgggaaac gtaaaaacct
     3121 gaccattttg atcgtcggag aaacgtcgcg cgcagagaat ttctcgctga atggttatcc
     3181 acgcgaaacc aatcctcgtc tggcaaaaga taacgtggtc tatttcccta ataccgcctc
     3241 ttgcggcacg gcaacggcag tttcagtacc atgcatgttc tcggatatgc cgcgtgagca
     3301 ctacaaagaa gagctggcac agcaccagga aggcgtgctg gatatcattc agcgagcggg
     3361 catcaacgtg ctgtggaatg acaacgatgg cggctgtaaa ggcgtttgcg atcgcgtacc
     3421 tcaccagaac atcaccgcgc tgaacctgcc tggtcagtgc atcaacggcg aatgctatga
     3481 tgaagtgctg ttccacgggc tggaagatta catcaataac ctgcagggtg atggcgtgat
     3541 tgtcttacac accatcggca gccacggccc gacctattac aaccgctatc cgccgcagtt
     3601 caggaaattt accccaacct gcgacactaa cgagatccag acctgtacca aagagcaact
     3661 ggtgaacact tacgacaaca cgctggttta cgtcgactat attgttgata aagcgattaa
     3721 tctgctgaaa gaacatcagg ataaatttac caccagcctg gtttatcttt ctgaccacgg
     3781 tgaatcgtta ggtgaaaatg gcatctatct gcacggtctg ccttatgcca tcgccccgga
     3841 tagccaaaaa caggtgccga tgctgctgtg gctatcggag gattatcaaa aacggtatca
     3901 ggttgaccag aactgcctgc aaaaacaggc gcaaacgcaa cactattcac aagacaattt
     3961 attctcaacc ttattgggcc tgactggcgt tgagacgaag tattaccagg ctgcgaatga
     4021 tattctgcaa acttgcagga gagtgagtga atgaaaattc tgattgttga agacgatacg
     4081 ctgttattgc agggattgat tctggcggcg caaaccgaag gctacgcgtg cgatggcgtg
     4141 agcaccgcgc ggatggcgga acaaagcctt gaggccggtc attacagcct ggtggtactg
     4201 gatttaggct tacccgatga agatgggctg cattttcttg cccgtatccg gcagaaaaaa
     4261 tacactctgc cggtgttaat tctcaccgcc cgcgataccc ttaccgacaa aatcgccggg
     4321 ctggatgtcg gggccgacga ttatctggtg aagccttttg cgctggaaga gttgcacgcc
     4381 cgtatccgcg ccctgctacg acgccataat aatcagggcg aaagtgagct gaacgttggc
     4441 aacctgacgc tgaatatggg tcgccgccag gtatggatga gcggagaaga actgattctg
     4501 acgcccaaag aatatgctct gctgtcccgc ctgatgctta aagcaggcag cccggtgcat
     4561 cgggaaattc tctacaacga tatctacaac tgggacaacg agccctcgac caacaccctg
     4621 gaagtgcata tccataacct gcgcgacaaa gtgggcaaag cccgtatccg caccgtgcgc
     4681 ggatttggct atatgctggt cgcaaatgag gaaaactaat tgaatctgat gcgttttctg
     4741 cgccgaccaa tatcgctgcg ccaacggctg atattgacca tcggggccat tttgttggta
     4801 tttgagctga tcagcgtctt ctggctatgg catgaaagta ccgagcagat tcagctgttt
     4861 gagcaggcgc tacgcgacaa tcgcaacaac gatcgccaca tcatgcgtga gatccgcgag
     4921 gcggtcgcca gcctgattgt ccccggcgtc tttatggtta gcctgacgct atttatctgc
     4981 tatcaggcgg tacgccgcat cacccgaccg ctggcggagc tgcaaaaaga gctggaagcg
     5041 cgcaccgccg acaacttaac gcccattgcc attcatagcg ccaccctcga aatcgatgcg
     5101 gtggtttcgg cgttaaacga tctggtcagt cgcctgacca gcacgctgga taacgaaagg
     5161 ttgtttaccg ctgacgtcgc gcacgaactg cgaacgccac tggcgggggt gcgtttgcat
     5221 ctggaactgc tggcgaaaac gcatcacatt gatgtagcac cgttagtggc acgacttgat
     5281 cagatgatgg agaacgtctc ccagctgctg caactggcgc gtgccggaca gtcattttct
     5341 tccggtaatt atcaacatgt aaaactgctg gaagatgtca ttctcccctc gtatgacgaa
     5401 ctcagtacca tgctcgacca gcgccagcaa accctgctat tgccagagag cgccgccgac
     5461 atcaccgttc agggtgatgc aaccctgctg cggatgttat tgcgaaacct ggtagaaaac
     5521 gcccatcgtt acagcccaca aggcagcaac attatgatta agctgcaaga agatggcgga
     5581 gcggtcatgg ccgttgaaga tgaagggccg ggtattgatg agagtaaatg cggggagttg
     5641 agtaaagcgt ttgtacgaat ggacagccgt tatggcggaa ttggtctggg gttaagtatt
     5701 gtcagccgca ttacacagtt gcatcacggg cagtttttcc tgcaaaaccg gcaagagact
     5761 tccggcacgc gggcctggat acggctgaaa aaagatcagt acgtggcaaa ccagatataa
     5821 agaaagctgc tgaccaccag cacgctgaac acggtagtta aactttcata aacacggttt
     5881 ttcattgcac tctcctccgt taacctggag gagagtatgc gcgtcagaga ttaagtgaac
     5941 cttaagagtt caattgacct gataagacag cgtcacatca ggccatccgt ttcagcttat
     6001 tcatcaattc gcggatgttg ctgcaccagg cgggtacgtt tcgcctgcaa atcggcaatc
     6061 tcgtggtcaa tatcatcgat tttctgctcg atattatcgt aatgctcgac gagaatttcc
     6121 ttcgcttcct gtatatctga cgccgctggt gtcgcacctt tcaacggtcg attcgccgtc
     6181 tctttcatgg ttacgccggt gattaaacca accaccgcca ctaccatcag gtaataggca
     6241 ggcatcatca gattctgcga gctttcgacc agccaggccg ccagcgttgg cgtcagaccg
     6301 gcaaccagca ccgaaatatt aaatgccgcc gccagcgcgc tgtaacggat atgcgtcggg
     6361 aacatcgcag gcaaggtaga agccataacg cccgtaaagc agttaaggat caccgccagc
     6421 atcaataagc cggcaaaaat caggccgatg acgttactgt taatcagaat aaacgccggg
     6481 atcgccaaca caaacaatgc aacactacca agaagcacaa acggacgacg gccaaaacgg
     6541 tcactcagca agcccatcac cggttgaaca aacagcatac cgatcataat ggcgataata
     6601 atcagcaccc cgtggtcttc ggagtaatgc aggttatgcg acaaataact cggcatatag
     6661 gtcagcaaca tgtagtaagt cacgttggtg gcaattacca gaccaataca tgtcaacagg
     6721 ctgcgccagt gtttggtggc aatctcttta aacgagactt tcgggccatc ctgcaaacct
     6781 tcgcggtcgc cctgttccag tttatcgaca tgctgctgga acgccggagt ctcttccagc
     6841 gcatggcgca ggtaaagccc gataatccct aacggcagag cgataaagaa cggaatacgc
     6901 cagccccaat cgaggaagtt ctcttcgccg acaatggtcg aaattaacac cactacgcca
     6961 gcacccagca caaacccggc aatagaacca aagtccagcc agctgcccat aaagccacgt
     7021 ttacggtcag gggagtattc cgcaacaaat attgacgccc cggtatattc accgccgacc
     7081 gagaaacctt gtgccatctt acagatcaac agcagaatcg gtgcccaaat accaatcgtg
     7141 tcgtaggacg gtattaagcc aatacagaac gtactgatcg acataatcac aatagtgata
     7201 gcgaggatct tctggcgacc atatttatcg cccaacatac caaagaagag cccgccaagc
     7261 ggtcgaatca gaaagggaac ggagaaagtg gcgagtgcag caaccatctg cacgctgggg
     7321 tcagcccccg ggaaaaaaac tttacctaat gcgtaagcaa caaaaccata aacaccaaaa
     7381 tcgaaccatt ccattgcatt acccagtgat gctgcggtaa tggctttacg cagtttacca
     7441 tcatcaataa tggtgacatc acgaagggta atcggtttta cttttttcct tttcagcata
     7501 gctttcctcg cagagttggc ccatttccgc aaacgccaga aaaatcagcg cgacggccgc
     7561 aaaaggggag cgggaattgc aggatgcaat ctctgtaaca agcgtagcgg gtttgcttac
     7621 accctccggt taaggaatgt acaatcaaac cagttgacgc ctgaattggg cagttaatga
     7681 cctttttacc ctaccagtgt ttaaatttgt gatcaacttc acacatgtat ttcgtttagg
     7741 actcattggt gtaactgatt atttacacca aatttacaga gaagatttcc tcgaccattg
     7801 ctcccgccag acgctgcgct gaacagagtc gtggcatccc cagtgcgaca gttcgccagc
     7861 gttgggtcac tgaccagcaa acgggttgtt tatctgccgt ggaccacttt cccagccaac
     7921 ttaaaaaatc agcatgctcc ccgcccgtcg aaacggtcga agaggcaaac cactgatggt
     7981 aatagtgacg cgtggtcggg gccgcattga tactctctgc caaatgttgt gtcgctttca
     8041 cgcgcaaatc acccagcaac aacgccaccg cttgcggtct tcccagacgg caggcatcgc
     8101 caaatgcacc ccagcgcaac tcctccagaa cgataaagca gcgcccaggc aacgaccagg
     8161 cgttatacgc cccagcacgc cagcgggtaa aaatttgctc gctctgttca cccgcctgca
     8221 ccaataggcc tcgttgcgtt aacgcctttt ccttccagcg acaacgggct gaaaaccact
     8281 catacaagtc gacaatttgc tgggggagct ccgggaaagc agagtgctgg cggtgatcca
     8341 ttttttgcag cagcgccaga tgctgcgcga tctgcgtcag cgccagatga ccgggatcca
     8401 gcatcgacgc ctgtttttcc accaatgtca gacgcaggct aagcggcgca tttaacatgc
     8461 cgccgttcgt ccatgtctga agctgcacgc gcgcaagaag ttcctgcatc agtcgttcac
     8521 gaaactgctg ctgatgggct tgtggaaggc gggcatcatc gccctgcgcc agatccacta
     8581 aaaagcgcgg atgaaccgac tccagcacgc gaccggggcc gtccagtaac gtcttggtca
     8641 atatcgttct gccgtgaaaa gtgtttgaat atcatcgcgt aacagctgag cgtcggtgta
     8701 aatccagccg tgagtcatca cagtctgctg caattgctgt tgcatcagcc tgaccacgga
     8761 ttcattttgt tgacgcagag ccagactttc gcgtaaacgc gtctgtaatt ccgtcaaaca
     8821 tgaagcgaac tcagcgaaaa aagtattcat gcctgccgta acagattcat cgacctgctc
     8881 tgccataact ttagccattt gttggcaata aagatcgact tctgcgctta atgctcgttg
     8941 caacacactg taatcaaccg tctctgtcgg gaatttctca tttccccgtc cccagtcggg
     9001 ctgattcaac cagcgcgaaa aaatctcacg cacaacgcca aaacgggtga tctgctcatc
     9061 cgttgctgcc tggcgcgaaa tgacttcact gaacagctgg cgggtgttga agtggggaac
     9121 tacgccgtga aaaacaggaa aatgaaaccc aggacgaaac ccagactcac tcaattccat
     9181 tttgacttgt tgctcaatgg ggcgaataac atcggttaac acccggcaaa gggtggactc
     9241 aagctcggca aagcgcagcg taaagtcgcg actgatggtg ttctgcgccg tctgtaacag
     9301 tgtctcacag cgggtacgca tctcacttaa cgactccgaa tcatcctgaa acaacgcggc
     9361 taactgcgca ttcaccgcat cttgctgttg acgcagaaag tggttggtgg aggtcagggc
     9421 caactcgatt tcatgtttaa tctcgccgct cacctgcgcc tgattgagtt gcaatagctg
     9481 caaactttct tcgacctgat ggatattttg ctgcaattgt tcacaagcga cgtttaaccc
     9541 gtgcgcacga aaatccaggt attcccgcgc ctgctgcgcg taattcaaca gtttatgcgc
     9601 ggcagatcgc agagcataca acgaggcgtt agcgtaagcg gcataaagca acgcctgaat
     9661 tggctgggcg aacagcgaat cttcccacaa ctgatcggca gcatggcgaa tatgttcgag
     9721 gtccgccaga tcggcatgac gccagcgcct gccgagcgcg gcatgggcaa aatcttccac
     9781 ccagcgttgt tgctctggcg cgggtaactt accgttgttg gctaactcat ggcgcgcccg
     9841 attcgccagg tagccccaca tcgacgacac cggaaatatc tgctgtggcg taatacagcc
     9901 tttcatcagc gttccggaaa tcagtgcccg cacctggtcg gcgtcgtcac tgttacgatc
     9961 ctgttgatcg aacttattga ccagcacata caacggcacc gattgcccca ccgccaaaat
    10021 tgcctcacgg acctcttcat cggagatcga tttcagttgc gtataatcca gcaccgccag
    10081 taccgccgag gcgcgtgcca gctgctggtt aagcattttt tgcagatgcg gttgcccggc
    10141 ttcatttggc ccgggggtat ccagtaacgt caactgaccg ggataactct ccagccccgc
    10201 cagatggaca aactccactt caatcacggg aatatgctca atggcggcgt aagcagaaaa
    10261 aggaaaatcg acgtccagcg ccttcgccag tcgcaccaaa tcattcaaac ttttcagaca
    10321 atgaaaaata ggttgagcac ccagataata tttttcgaaa gcgatgccat tttcgatccg
    10381 ctgcataagc gcacgcatat ctttatctat ttccagcaca tcggtcagat gcttaatatc
    10441 gcaatcacgc aggcgctgtt gtaattgttg aattaaatgg tcgattggcg cgacatgtga
    10501 aaaatgcagt accggttctt tttgcccggg cgtatggcga ataagcgttg gcagcgcagt
    10561 cattgggcga ttgcgattag gcagaacctc cgtaccaaca atggcattaa tggtggttga
    10621 tttccctgct ttcatggtac cgacaatcgc cagcaccatt tccagctggg aaattttacg
    10681 caactcatta ttcagcatcg cgtgacgttc ggcgatatta ggctgactcc agggtaaagc
    10741 cagttgtggc gcatcgtctc cgggtacaga gagaggcatt ttttccagta actgcaactg
    10801 ttggcgagaa agctgtaaca ggcgttcagc ctcctgactt aactcataca gggtctgtgt
    10861 gtacatagaa aattcttcct taaagcaaat tttattactt ttatttagcc agattgcttt
    10921 tgagttctgt tttcggcttt tataattact gcaagaaata attttat
//
LOCUS       DAZGJT010000129        10919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZGJT010000129 DAZGJT010000000
VERSION     DAZGJT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.129"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..634)
                     /gene="bet"
                     /locus_tag="ACHKFK_004125"
     CDS             complement(<1..634)
                     /gene="bet"
                     /locus_tag="ACHKFK_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9054350.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIA"
     gene            complement(640..936)
                     /locus_tag="ACHKFK_004126"
     CDS             complement(640..936)
                     /locus_tag="ACHKFK_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9054351.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAIIRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            complement(1010..1153)
                     /gene="kil"
                     /locus_tag="ACHKFK_004127"
     CDS             complement(1010..1153)
                     /gene="kil"
                     /locus_tag="ACHKFK_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO9054352.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
     gene            complement(1146..1286)
                     /locus_tag="ACHKFK_004128"
     CDS             complement(1146..1286)
                     /locus_tag="ACHKFK_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO9054353.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            complement(1511..2134)
                     /locus_tag="ACHKFK_004129"
     CDS             complement(1511..2134)
                     /locus_tag="ACHKFK_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO9054354.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDQLASKLMAASEELQKLGCSFYSCDVSSCSIYLTVSGRRVGWHSYG
                     KDGNGEDILLPTPDKDKCMFDAEHKITKRFDEICALQQKLEARKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASAALPALRVEDLNKMIGLPSEAA"
     gene            complement(2196..2468)
                     /locus_tag="ACHKFK_004130"
     CDS             complement(2196..2468)
                     /locus_tag="ACHKFK_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO9054355.1"
                     /translation="MSRKTEFKGTAASRRRARRANLQSQESISSDKLHRPTPSRVVLQ
                     CKRKPAMRAEVITLTTLTRKYEGSTCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(2446..2671)
                     /locus_tag="ACHKFK_004131"
                     /pseudo
     CDS             complement(2446..2671)
                     /locus_tag="ACHKFK_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502599.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2974..3594)
                     /locus_tag="ACHKFK_004132"
     CDS             complement(2974..3594)
                     /locus_tag="ACHKFK_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054356.1"
                     /translation="MSDDSNLNNHGKQDTNISESRRLKVVGGSDFEAEFDNSPTKVQN
                     NYIKPPQTEEEVGTISREELDARLAANKAEMESIASSIRTDMALSRESVNVQFASLNA
                     AISSLSSKIDGKMDSAAGDIKAINGRFEGIQGQITGVNTAISGVQSGISIRLAIFSVI
                     IAVIVAIPGLVSALKSDHAPLQQPSTLQAPPQNTQPTDNKKITPQH"
     gene            complement(3591..4079)
                     /locus_tag="ACHKFK_004133"
     CDS             complement(3591..4079)
                     /locus_tag="ACHKFK_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054357.1"
                     /translation="MMDGRRDARRAYSDAGEFMALNLERISFITPFDSSEEPNQPTLN
                     FTCNEFPARLSIDFRVGMIGLKPNSRYNLGIMVIPAHLIIKKGEEIQFPDGSSESVSL
                     FIDTKDSHFETGVGGQVIVTLKEIRVPAKGLYSVIGILQDNEDPKNELHKNESFFTVE
                     LL"
     gene            complement(4076..4723)
                     /locus_tag="ACHKFK_004134"
     CDS             complement(4076..4723)
                     /locus_tag="ACHKFK_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO9054358.1"
                     /translation="MKTMHEIIGERIKSLREAKGLSQAQLAKLCGWAAPSRLGNYELG
                     TRKVSADDALVLGAALGVSPAKIMFGEDSDAVFRQYEYPLFSSVQAGPFSEVGSYTAS
                     DAKAWVPTTTKASEKAFWLEVKGHSMTAPQGVRPSFPEGMLILVDPAEPVESGDFCVA
                     SANGDSEATFKKYEKDAGVSYLVPLNPAYRTLDCDHTCRIIGKVVKAQWPEETFG"
     gene            4832..5050
                     /locus_tag="ACHKFK_004135"
     CDS             4832..5050
                     /locus_tag="ACHKFK_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9054359.1"
                     /translation="MNKIAQQRKKIGVSQAVLASAIGWGQSRIANYELNIRTPRLNDC
                     RKIVEGLRKLGCQCSLDDVFPPSSNKAA"
     gene            5190..5489
                     /locus_tag="ACHKFK_004136"
     CDS             5190..5489
                     /locus_tag="ACHKFK_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9054360.1"
                     /translation="MEQTSYSKLSQRDVDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDELTNKKRPVCKTERSEQIQMEF"
     gene            5522..6460
                     /locus_tag="ACHKFK_004137"
     CDS             5522..6460
                     /locus_tag="ACHKFK_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9054361.1"
                     /translation="MTNTAKILNFGRSNFAGQERNVADLDDGYARLSNMLIEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGTPPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAGVTAGKPKLDLTNTDWIYGVDL"
     gene            6457..7158
                     /locus_tag="ACHKFK_004138"
     CDS             6457..7158
                     /locus_tag="ACHKFK_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9054362.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRGQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            7155..7445
                     /locus_tag="ACHKFK_004139"
     CDS             7155..7445
                     /locus_tag="ACHKFK_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO9054363.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKVSTNLIFKECRQSAVMKRVLAVYGVKR"
     gene            7531..7746
                     /locus_tag="ACHKFK_004140"
     CDS             7531..7746
                     /locus_tag="ACHKFK_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054364.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            7757..7993
                     /locus_tag="ACHKFK_004141"
     CDS             7757..7993
                     /locus_tag="ACHKFK_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO9054365.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            7950..8396
                     /locus_tag="ACHKFK_004142"
     CDS             7950..8396
                     /locus_tag="ACHKFK_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO9054366.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSAPGIGGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSWREYEWSQRKGKAA"
     gene            8393..8920
                     /locus_tag="ACHKFK_004143"
     CDS             8393..8920
                     /locus_tag="ACHKFK_004143"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9054367.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            8917..9099
                     /locus_tag="ACHKFK_004144"
     CDS             8917..9099
                     /locus_tag="ACHKFK_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9054368.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKPRPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            9378..10091
                     /locus_tag="ACHKFK_004145"
     CDS             9378..10091
                     /locus_tag="ACHKFK_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22AR C-terminal domain-containing protein"
                     /protein_id="HFO9054369.1"
                     /translation="MATQISVETLSPIAYNQIPVITTELLAQLYGTETVNIKMNFSRN
                     SARFVQGKHFFKLEGNELREFKHRLSLSESVSREVTESYSVKIARNVRSLILWTERGA
                     ARHAKMLETDQAWEVFEKLEDCYFSQCEKNTGKQEKKPNGLSAKETDSLVWLWDYANR
                     SQALFRELYPALKLIQSGYSGICHDYGYEFSYTIGRARGVLINHTRDIDIYEPDGPTN
                     LLAWERLKNKELPPSLHRY"
     gene            10166..10888
                     /locus_tag="ACHKFK_004146"
     CDS             10166..10888
                     /locus_tag="ACHKFK_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO9054370.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNYAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMERGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTGEAA"
BASE COUNT         2915 a         2523 c         2685 g         2796 t
ORIGIN      
        1 gggcgatcag cagagtgtta atctcctgca tggtttcatc gttaaccgga gtgatgtcgc
       61 gttctggctg acgttctgca gtgtatgcag tattttcgac aatgcgctcg gcttcatcct
      121 tgtcatagat accagcaaat ccgaaggcca gacgggcaca ctgaatcatg gctttatgcc
      181 gtaacatccg tttgggatgc gactgccacg gtccggtgat ttctctgcct tcgcgggttt
      241 tgaatggttc gcggcggcat tcatccatcc attcggtaac gcagatcgga tgattacggt
      301 ccttgcggta aatccggcat gtacaggatt cattgtcctg ctcaaagtcc atgccatcaa
      361 actgctggtt ttcattgatg atgcgggacc agccatcaac gcccaccacc ggaacgatgc
      421 cgttctgctt atcagggaag gcgtaaattt ctttcgtcca cggattaagg ccgtactggt
      481 tggcgacgat caacaatgcg atgaactgcg catcgctggc atcgccttta aatgccgtct
      541 ggcgaagagt ggtgatcagt tcctgtgggt cgacagaatc catgccgaca cgttcagcca
      601 gcttccctgc cagcgttgcg agtgctgtac tcatccgttt tatacctctg aatcaatatc
      661 aacctgatgg tgagcaatgg tttcaaccat gtaccggatg tgttctgcca tgcgctcctg
      721 aaactcaaca tcgtcatcaa acgcacggat aatggctttt ttgctggccc cgtggcgttg
      781 caaatgatcg atgcatagcg attcaaacag gtgctggggc aggccttttt ccatgtcgtc
      841 tgccagttct gcctctttct cttcacgggc gatctgctgg tagtgacgcg cccagctctg
      901 agcctcaaga cgatcctgaa tgtaataagc gttcatggct gaactcctga aatagctgtg
      961 aaaatatcgc ccgcgaaatg ccgggctgat taggaaaaca ggaaaggggt tagtgaatgc
     1021 ttttgcttga tctcagtttc agtattaata tccatttttt ataagcgtcg acggcttcac
     1081 gaaacatctt ttcatcacca ataaaagtgg cgatagtgaa tttagtctgg atagccataa
     1141 gtgttttatc catttttggg aactcctggc tgattaagta tgtcgataag gcgtttccat
     1201 ccgtcacgta atttacgggt gattcgttca agtaaagatt cggaagggca gccagcaaca
     1261 ggccaccctg caatggcata ttgcatggtg tgctccttat ttatttacac ataacgaaaa
     1321 cgcctctagt gaagcgttat tggtatgcat ataaaaaagc cctcacattg gagggcaaag
     1381 aagatttcca ataatcagaa caagtcggct cctgtttagt tacgagcgac attgctccgt
     1441 gtattcactc gttggaatga atacacagtg cagtgtttat tctgttgttt atgccaaaaa
     1501 taaaggccga ttatgcggcc tcggaaggaa gtccaatcat cttattcaaa tcttctaccc
     1561 gtaaagcagg aagtgctgca cttgctttat ctgcttcttt tggtagcaac tctttgcttt
     1621 caggccaaac ttcaataagt cgcttaactg ttgtgactga gttcaaagca gcccatacgt
     1681 ttgattctat atcctttttc ctggcttcaa gtttttgttg caatgcgcag atttcatcaa
     1741 acctttttgt tattttgtgt tctgcgtcaa acatgcattt atctttgtcg ggggtaggga
     1801 gcaatatatc ttcaccgttg ccgtcttttc cgtatgaatg ccagccaacc cttcttccag
     1861 atacagtcag ataaatcgaa catgaactga catcgcatga gtaaaatgaa catcccagct
     1921 tttgaagttc ctcacttgca gccattaact ttgatgccag ctggtccact tcttcggttt
     1981 tctttttccc gccaaacgca ataactctgg cgtcaagtgc aagctggttc tttaactttg
     2041 ttacttcttc aagttcagtg aacaccccgg acttaattaa agcgttacga gcgatttcct
     2101 ctttcattct cgtagttaag cggattgatg acatattaat tcctctcaaa taagtggttt
     2161 gctgcctaat ttcattttct ggcgaccaac acaagtcatc ttgctgtcag ttgtttggat
     2221 ttacggtagc ctgcagcgta aatggctacg tttggaagac aagttgaacc ttcatatttt
     2281 ctggtcaacg ttgtcagagt tataacttct gctctcattg ctggtttgcg cttgcattgc
     2341 aagaccactc gtgaaggggt tggcctgtgt agcttgtcgg agctaatcga ctcctgactt
     2401 tgcaggtttg cgcgacgagc tctacggcga gaagctgcgg tgcctttaaa ttctgttttt
     2461 ctggacatag attcctcccg aataaacttt ggcgatgcaa tctcgaagct cctcctgaga
     2521 cggttgcttc ggcattgcat cccacagctt atgtggttgg gtgatctggc ttttcagcca
     2581 cgtagtcgag tgttcgacgt tgtttaaaga gcctgccaat cttttccgtt tggctaccag
     2641 cgtcctgctg atggaaataa tagtcacata ttgtgattta atggtcaatc acaaaatgtg
     2701 taaaataagg gtgtgacacg ttatgtgtat gatttttttg tgtaaatagt tttccccgcg
     2761 atgggtttgc ttacttaagg cgggggaaac agcaggatgg tgtgctgaaa agttcgaaaa
     2821 acgagcgaag taggtggtga cgagatggcg ggatatggga gttgtattaa gaattatagt
     2881 aatttaatca gaggcttggg ctggctggca ggtgctgtcc tgatagttat gtgcaggcaa
     2941 caaaaaaccc ggcgctgagg ccgggttaat gcgttaatgc tggggagtga tttttttgtt
     3001 gtctgttggc tgggtatttt gtggcggcgc ttgcaaagtg gaaggctgtt gcaaaggtgc
     3061 gtggtctgac tttaaagctg ataccagccc gggaatcgca actattacag cgataatgac
     3121 actaaaaatg gctaatcgta tagaaatacc cgactgaaca ccactaattg cggtattaac
     3181 ccctgttatt tgtccttgaa ttccttcgaa tctcccatta attgctttta tatcgccagc
     3241 agcgctatcc atctttccat cgatcttgga cgatagagag cttatggctg cattaagtga
     3301 tgcaaattgg acattaacgc tttcacgaga tagagccatg tcagtcctaa tggaagaagc
     3361 aatagactcc atctctgctt tattagcagc taagcgagca tcaagttcct ctctgctgat
     3421 cgttcccact tcttcctccg tttgtggcgg ttttatgtaa ttattttgca ccttggtggg
     3481 agaattatca aattcagcct caaaatcgga accgccaaca accttcaacc tccggctttc
     3541 agatatgttg gtgtcttgct ttccatgatt atttaaatta ctgtcgtcac tcataatagc
     3601 tctacggtga aaaatgattc atttttatga agttcatttt tagggtcttc attatcttgc
     3661 aatatcccta taacactata cagcccttta gctgggaccc taatttcttt caatgttact
     3721 attacctgtc cgccaacccc tgtttcaaaa tggctatctt tcgtatcgat gaaaagtgaa
     3781 actgattccg aagagccgtc agggaattga atttcctcac ctttttttat aattaggtgc
     3841 gcggggatta ccataatacc caaattatat cttgaatttg gtttcaaccc gatcatacca
     3901 accctgaaat caatcgatag ccgcgcagga aattcattgc atgtaaaatt aagcgtcggt
     3961 tggttgggtt cttcgctgct atcaaatggg gttataaaag atattctttc taaattaagt
     4021 gccataaatt ctcctgcatc gctgtatgct ctacgagcat ctctgcgacc atccatcatc
     4081 caaacgtctc ttcaggccac tgagccttaa ctaccttgcc tatgatgcgg catgtgtgat
     4141 cgcagtccag ggttctgtat gccggattca atggaaccaa gtaactaacc cctgcatcct
     4201 tctcatactt cttgaacgtt gcctctgaat caccatttgc agaagccacg cagaaatccc
     4261 cagactctac cggctcggcc ggatcaacga gtatcagcat accctcagga aaactcggcc
     4321 ttacgccctg tggcgcagtc atagaatggc ctttcacctc aagccagaaa gctttttcgc
     4381 tggcttttgt ggtcgttggg acccatgcct ttgcatcgct tgctgtgtag cttccaacct
     4441 ccgaaaacgg cccggcctgc actgaagaaa ataacgggta ctcatattgg cgaaatacag
     4501 cgtctgaatc ctcgccaaac attatttttg ccggagatac gccgagtgcc gccccaagaa
     4561 ccagcgcgtc atccgcgcta acctttcttg ttcctaactc gtagttcccc aggcgtgaag
     4621 gcgcagccca gccgcaaagc ttggccaatt gagcctggct aagtccttta gcttctctaa
     4681 gggacttgat cctttccccg ataatttcat gcatcgtttt catcctttaa atgtaacacg
     4741 caacgtgatt gaactctgta cacgaattga ggttgactgt taatcacaaa ttgtgtgtaa
     4801 tgggtgtgtg attaatacta gggagaccgc aatgaacaaa attgcccagc agcgaaaaaa
     4861 aatcggagtt tcgcaagctg tactggcttc ggcaattggt tgggggcaat cccgcatcgc
     4921 caactatgag ctgaatatcc gtactcctag acttaacgat tgccgaaaga tcgtagaagg
     4981 cctcaggaag ttagggtgcc aatgttcttt ggatgatgtt ttccctccat ccagtaacaa
     5041 agccgcctaa gcagtatccg ctctttgtaa caacggacat tcgtcctacg tcgctgaaaa
     5101 gcgagtccca agatatctga ccaactaagg ccatatgcgt ttccacgcat acctttcaac
     5161 taactattca ctattggaaa tcttaagaaa tggaacaaac aagttacagc aaactatcac
     5221 agcgcgacgt tgatcgcgca gaaacagatt tacttatcaa cctgtcaacg cttacccagc
     5281 gcggtctggc aaagatgatt ggctgtcatg aatcgaagat aagcagaacg gactggagat
     5341 ttattgcttc ggtcttgtgt gctttcggaa tggcatcaga catcagtccg attagcaggg
     5401 cttttaagta tgcgcttgat gaactcacca ataaaaaacg cccggtgtgc aagaccgagc
     5461 gttctgaaca aatccagatg gaattctgag gtcattactg gatctatcaa caggagtcat
     5521 tatgacaaat acagcaaaaa tactcaactt cggcagaagt aactttgccg gacaggagcg
     5581 taatgtggca gatctcgatg atggttacgc cagactatca aatatgctga ttgaggctta
     5641 ttcaggcgca gatctgacca agcgacagtt taaagtgctg cttgccattc tgcgtaaaac
     5701 ctatgggtgg aataaaccaa tggacagaat caccgattct caacttagcg agattacaaa
     5761 gttacctgtc aaacggtgca atgaagccaa gttagaactc gtcagaatga atattatcaa
     5821 gcagcaaggc ggcatgtttg gaccaaataa aaacatctca gaatggtgca tccctcaaaa
     5881 cgagggaggt tcccctaaaa tgagggacat ccctcaaaac gagggaaccc cccctaaaac
     5941 gagggataaa acatccctca aattagggga ttgctatccc tcaaaacagg gggacacaaa
     6001 agacactatt acaaaagaaa aaagaaaaga ttattcgtcc gagaattctg gcgaatcctc
     6061 tgaccagcca gaaaacgatc tttctgtggt taaaccggat gctgcaattc agagcggcag
     6121 caagtgggga acagcagaag acctgaccgc cgcagagtgg atgtttgaca tggtgaagac
     6181 catcgcacca tcagccagaa aaccgaattt tgcagggtgg gctaacgata tccgcctgat
     6241 gcgtgaacgt gacggacgta accaccgcga catgtgcgtg ctgttccgct gggcatgcca
     6301 ggacaacttc tggtccggta acgtgctaag tccggccaaa ctccgcgaca agtggaccca
     6361 actcgaaatc aaccgtaaca agcaacaggc tggcgtgaca gctggaaaac caaaactcga
     6421 cctgacaaac actgactgga tttacggggt ggatttatga aaaacatcgc cgcacagatg
     6481 gttaactttg accgtgagca gatgcgtcgg atcgccaaca acatgccgga acagtacgac
     6541 gaaaagccgc aggtacaaca ggtagcgcag atcatcaacg gtgtgttcag ccagttactg
     6601 gcaactttcc cggcgagtct ggctaaccgg ggccagaacg aactgaatga aatccgccgc
     6661 cagtgggttc tggctttccg ggaaaacggg atcacctcga tggaacaggt taacgcagga
     6721 atgcgcgtag cccgtcggca gaatcgacca tttcttccat cacccgggca gtttgttgca
     6781 tggtgccggg aagaagcatc cgttatcgcc ggactgccaa acgtcagcga gctggttgat
     6841 atggtttacg agtattgccg gaagcgtggc ctgtatccgg atgcagagtc ttatccgtgg
     6901 aaatcaaacg cgcactactg gctggttacc aacctgtacc agaacatgcg ggccaatgcg
     6961 ctgactgacg cggaattacg gcgcaaggct gccgatgaac tgacctgtat gacagcacga
     7021 attaaccgtg gtgagacgat acctgaacca gtaaaacaac ttcctgtcat gggcggcaga
     7081 cctctaaatc gagcacaggc tctggcgaag atcgcagaaa ttaaagctaa gttcggactg
     7141 aaaggagcaa gtgtatgacg ggcaaagagg caattattca ttacctcggg acgcataaga
     7201 acttctgtgc acaggacgtt tccgcggtaa caggcgcaac cgtaaccagt ataaatcagg
     7261 ctgcggctaa aatggcgcgg gcaggaatcc ttgtcattga tggtaaggtc tggcgaacgg
     7321 tgtattaccg gtttgctacc agggaagaac gggaaggaaa ggtgagcacg aacctgattt
     7381 ttaaggagtg tcgccagagt gcagtgatga aacgggtatt ggcggtatat ggagttaaaa
     7441 gatgaccatc tacatcactg agcttgtaac aggcctgctg gtaatcgcag gcctttttat
     7501 ttggggaaga gtaattggag gctttaagaa atgagtacga tagctgagct tgtcagggct
     7561 aattttcgtg aagagttggt gcgttggtat cggtatcgtt catcgtccag tttgccgctt
     7621 gatgagttgt atgagcattc acctgccgca cgacgctatc cgcgtgaccg tgttcttcga
     7681 cggttgttca aactcaacaa tgagtttcag cgcaacagaa ttatccggag tctggattta
     7741 aagtgaagga gtgagcatga gcgacctatc attaacccag ccaaagctaa aagaatgtcc
     7801 gttttgcggc ggtaatgctc gtctgtgggt tgaggccgga ataaatattg atgtgtgggg
     7861 ctatgcagaa tgtgacctct gtgaagccag gggggcatgg gcaccatcag ttgctgcggc
     7921 ggctgaaaaa tggaaccgga gagcaggaga tgaagcaaac ctttctgctt cgcaacgaag
     7981 caatcagaaa taacgccata gacgccattc tctcactacc catcgacgac aagtcacccc
     8041 acgaagtcca cgttaaagaa cccaagcgta gcaaagcgca gaatgaccgt atgtggccga
     8101 tgctgaacga tgtttcgcgt caggtgctat ggcatggtca acggctggcg ccggaagact
     8161 ggaaagacct gttcactgcc ctgtggctta agaccaaaaa actggagcaa cgaagtgcgc
     8221 ctggtatcgg cggtggcgtt gtcatgcttg gtgtgcgcac cagcaaaatg cggaaggcca
     8281 gcatgactga gcttatcgaa atcatgttct ggttcggctc agagcgcaac gtgcggtgga
     8341 gtgatgactc ctggcgagag tatgaatggt cacaacgaaa agggaaggct gcatgactat
     8401 caaatcaaat acgccagcac acgacaagga ctgctggcaa acgccgcttt ggctttttga
     8461 tgcactggat attgagtttg gattctggct ggattcggca gcgagcgaca aaaatgctct
     8521 gtgtgctcac tggctaactg aggccgacga cgcactcaat tctgagtggg taagccacgg
     8581 tgcaatctgg aataacccac cgtacagcaa tatcaggccg tgggtggaaa aagccgctga
     8641 gcagtgcata caacaacgac agacggtagt gatgcttgtg ccagaggata tgtctgtcgg
     8701 atggttcagt aaggctctgg agagtgttga cgaagttcgc atcatcactg atggacggat
     8761 taattttatc gaaccatcga cagggctgga gaagaaggga aacagcaaag gttccatgct
     8821 gctgatttgg cgaccgttca tcagtcctcg acggatgttt actaccgtat ccaaagcggc
     8881 attgatggcg atcgggcagg gcgtcaggag ggcggcatga ggcgacagcg acgaagtatc
     8941 accgacatca tctgcgaaaa ctgcaaatac cttccaacga aacgctccag aaataaaccc
     9001 aggccaatcc caaaagaatc tgacgttaaa accttcaatt acacggctca cctgtgggat
     9061 atccggtggc taagacatcg tgcgaggaaa acaaggtgat tgacgtgatg atttattcgg
     9121 ggctatattc ctcacgcgcc agcaaaatct ggcgtcggga tttgcacccc ggatattcaa
     9181 ccgcgacaga cacacgccgc gagcgtgttt tttgttgtcg taagcacacg cacatctgaa
     9241 ttatggtggg gcgtatgggg gagccgaaag gctcgccggt tggttgatcc ggtagtgcaa
     9301 accctgtacg tctcaccacc caaagatttg cacctgacgg tggtgatagt taaattcatc
     9361 aaccagaggg cgttgtcatg gcgactcaaa tttccgttga aactctttcc ccaattgcct
     9421 acaaccagat ccccgtaatt actacagaat tattggcgca attgtatggc actgaaacag
     9481 tcaatataaa aatgaatttt tcccgtaact ctgcgcgatt tgtacaggga aaacatttct
     9541 ttaagttgga agggaatgaa ctacgtgagt ttaagcacag actatcttta agcgagtctg
     9601 ttagccgcga ggtaacagaa agttactctg tgaaaatcgc ccgcaatgtt cgctccctca
     9661 tcctctggac agaacgcggc gcagcccgtc acgcaaaaat gctcgaaacc gatcaggcgt
     9721 gggaagtgtt cgaaaaactg gaagactgct atttcagcca gtgcgagaaa aatactggca
     9781 aacaagagaa gaagcccaac gggctttccg caaaagaaac agacagcctt gtatggctgt
     9841 gggattatgc caaccgctca caggcattgt tccgtgagtt gtatcccgca ttaaaactga
     9901 ttcagtctgg ctattccggc atatgccacg actacggcta tgagttctcg tataccatcg
     9961 ggagggcgag gggcgtttta attaatcaca cgcgggatat agatatttat gagcctgacg
    10021 ggccgacgaa ccttctggca tgggaaaggc ttaagaacaa agagttgccg ccttcactgc
    10081 atcgctactg acaattgaca acttaacaaa cccagcttcg gctgggtttt ttattgctga
    10141 attttcaata tgagaggaca tgacaatgaa tgagctgata aatagcaatg ccatcaaaat
    10201 gacaagcatt gaaatcgctg agttggtggg aagtcgtcat gacaaggtga aacaatccat
    10261 tgaacgactg gcggttcgag gtgtgatccg aaatccccca atggtggttt tcgaaaaaat
    10321 caataactta ggattacttc gtggcgtaga ggcttacgtt tttgagggcg aacaaggtaa
    10381 gcgcgacagc attattgtcg ttgcccagtt gtcgccggaa ttcaccgctc gtcttgttga
    10441 ccgttggcga gaactcgaag gggcaaccgc gaaaatacca caaacctttt ctgaggcatt
    10501 gcgccttgcg gccgaccttg aagaccagaa ggctgaactg gagaaacagc ttgctctcgc
    10561 agcacctaaa gttgagtttg ccgatcgcgt tggcgaggcc agcggaattt tgattggaaa
    10621 ctatgcaaag gttgttggaa ttggtccaaa caaactgttt gcgtggatgc gcgatcacaa
    10681 aatccttatc gcttcaggtt cccggcgaaa tgtgccaatg caggaatata tggagcgcgg
    10741 ctatttcaca gtgaaagaaa cagcggtcaa cacaaatcac ggaatacaga tatcgttcac
    10801 cacaaaaatc accgggcgtg gtcaacagtg gctgaccaga aagctgctcg ataacggaat
    10861 gctgaaagta acaggggagg ctgcttaatg gctaaaccag cgcgaaggaa atgcaaaat
//
LOCUS       DAZGJT010000130        10905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZGJT010000130 DAZGJT010000000
VERSION     DAZGJT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.130"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            251..871
                     /locus_tag="ACHKFK_004147"
     CDS             251..871
                     /locus_tag="ACHKFK_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9054371.1"
                     /translation="MVLITTTKNIFIIQAIKSMRWRGFSYVKVIAVDDYSSLIRDFGT
                     SCFRDEQSLLLPVFPDNDSTTCIRSLYFIHQWLKSQPVNIPCLAWGDNGVVSNKGIPF
                     IPWKLTAEQFCFYIDKFINGWRCKKNIRANHKVSFLERQLSPREKSIFIYTKEGKDIA
                     WISNTLGISSKTVWTHRRRAMDKLGVKRLHQFINIPRSLISENIQL"
     gene            896..8809
                     /locus_tag="ACHKFK_004148"
     CDS             896..8809
                     /locus_tag="ACHKFK_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phase-variable autotransporter adhesin UpaE"
                     /protein_id="HFO9054372.1"
                     /translation="MNKIYKVIWNHTTQKWDVVSELTSCRKKCKSTRLGIALSAMVLG
                     GAIAINCNNAMADVILSPDWRPGTNNSGVGAATVSGKTEYITGPNVVQSGGSGLIWMT
                     VEQAILNGYTTGDNLSGLIYVNTGEKTKTITVKDEVTGASQTLQVFDTDSFSQRDAGT
                     GGNETIPGFSGTADFFNATRFVTANNGGTAILDVGSPAIGNFFKNTQLAVADGEGSSV
                     VWNSVNDFYFQPGATMQGGGVTQKIIDSMKYAGTITDWAGKVHHINSLDDLKQYNQYL
                     IKSLEDKTLSYKQYDAEFNKALIVTKHNYNVDMTAGGRIDSTPYKENVGLLAVLHATN
                     NARAILGKTGKLTGVLPAYGNGGGIVATNGGTGVNEGVIDAIGTEMIAYQDSTIVNDG
                     TLFVWDNNDKYALQAEGMVAGSNGSSAINNGVINIRPFKNAFAPEGINTAIVVSNGGM
                     ATNKGTINITADASTNDNNGKTRGVNVGAGGSFINSAFGSINVGIAEDKTATHSAVGS
                     VAIEVQNGANKVVNEGTIFLGRGAQGNYGILAKDAGTVDVVNKGTITIDGHDSDAPAL
                     NVGMLANNSSGMKNSGIINVNGLNSTGLQVINAGQLNSDGTINVGGKGISSGFRNYGA
                     WVEGAGSNVNVSGKISLAGTGAVGVFAKDGGSLTLSGNGAVLFGSSDQIGFYVYGKDS
                     AIHNTGSGVMDVSTENSTLFRIASGATFQGTADASSALTASGKNSYALIATGKSDGGV
                     ASTVTSGGMTINLTGEGATATLIEGGAQGTIESNAIINMDNASAIAGIADGNGYDISG
                     KLINPKDKTTLLTAGAQLSSTQDKVTGYIARNGATLNNTGNIIFTGKNTVGVRVEEGA
                     VGTNSGNITVQDGGVGLIANATQDVTTINNSGNLVLKGGDNANRTTGIKASGTTTTVN
                     MTAGTISLQGQGAIGVEASNKGTVNLDGSAVPNFAADGSGITDQIAFRIIGDGATIKT
                     NIAPGTLLDASGERSVLFRIEDGAKQAGSLLMKTSGTGSRGIWATGKGSNVLADAGSD
                     FQILGAQAQGLYVTGGATATLKQGASVNLVGDGAVVAEVDGNEYALDGSITQTNTGSV
                     ITNEADISSPLNNARGFITRNQGLLINNGNIDFTAGTDNIGVWVDNGRFENTGSRIAV
                     NGVALFVEGAQSQITSTGGDIVAVDGEAAIKLGAGASLNLAGSGLGTIEGQKNAHGIL
                     LDTGAVGLVIDGAKINVNAAGAVGHGIENRAEIEGIQLTNTTEINVADGIGVRTSASL
                     AKTNSGTINVDGSGIALAFQKADGSETDNNLDMSDSAGLVINLKGTDGTGIFANTKDG
                     AVVKSGASVNVTQADGGSALVVNNAASEVVQSGNLISASLSHAVVDASKAQSFTNKGQ
                     IKAASTTGTAMAFDDAVNTTVLNDSGAEIQGVVVLNGGDNTFTNKGSITGTVSAKEGN
                     NTFLFDDGSTLTGEVTAGNGNNNVTLNGKAHVDQVTAGTGKNTFTIKGEGATWNLLDG
                     GQGDSDSLIFDNAIHTLDSVVKLQNFEHVGLKNSSLVTLKEALVLTDGGNGPGSVDIE
                     SGSELAIIPAVAGNFTFDPLLTGKGTLSARLDADTSAFEFSHNVGDQFAGTLKLGTSS
                     FALEGLNTSGLTHAMLMSETGNITTVGSGVQQIGGLGFNGGTLIFGSVMPGDTIASNS
                     IETSAAGTLDIRGKGTIQVTMPDEVINDIPAVDTRKNLLEQDDAQTLVTLVNAAGTVT
                     GTGGQLQLVDENGQAISHSQTFDVTQGGEVVAQGNYDYKLLGSSDGIKGDGLYIGYGL
                     KSLDLQGTGDKALVLTPRANAQGLQTDLGAQLTGAGDLAIEAAGQVVTLSNGGNNYTG
                     DTLVRSGTLQMANDNVLGATGNLNVASNGVFRTNGYSQTVGALQTETGAHIQLDSGSV
                     LTVSGTQRQPGDDNGGIIENNVLSGEGTLAVTGSNLTVHGTNIGFTGNASLTQGALVE
                     MNGAQGLGSQGSISFESLNDRLAIDIADGSGVSSNLSKSLSGEGSVGILNTTDLTLSG
                     DNSNFSGEFRVQKDAALRASDEKHLGTGLIDSDGVTWLTASGNWLLKNDITGSGALVK
                     QGAGNLIINHELTYTGDTTVESGVLIVGDDSVTRAAGATLSGSKNIHVLNGGILSGLG
                     TVSGQVNNQGTLASLNALSGYETAEVGNFTVGSLTNTGVIRLAGGKTGNTLTVNGDYT
                     GGGTLIINTVLGDDTSATDKLIVTGNTSGDTGVVVNNVRGQGAQTADGIEIVHVGGQS
                     DGNFRLQNRAVAGAWEYFLHKGNAGGTDGNWYLRSELPPEPQPQPQPQPQPQPHPTPD
                     KPVQKVYRPEAGSYIANIAAANTLFNIRMHDREGETYYTDVFTGEKKATSMWMRHIGG
                     HNRWKDSSSQLNTQSNRYVVQLGGSIAQWTDGQDRLQLGIMAGYGNEKSSTTSSLSGY
                     KSKGAINGYSTGLYGTWQQNDGNDNGAYVDTWIQYGWFNNTVNGEKLAAESWKSRGFT
                     GSVEAGYTFKAGEFTGSQGSHYDWYIQPQSQITWMNVRASEHTEKNGTKVQLSGDGNI
                     QSRLGVRTYLKGKSASDDNKAHQFEPFVEVNWIHNTRSWGVKMDNTALSQDGATNIAE
                     VKTGVQGKLSDNLNVWGNVGVQAGDKGYSDAQAMLGIKYIF"
     gene            8869..9387
                     /locus_tag="ACHKFK_004149"
     CDS             8869..9387
                     /locus_tag="ACHKFK_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFO9054373.1"
                     /translation="MKFSVVNAVVLLLLIVLTGVFYMKKSKEIAVVNIDAIYNQSAAG
                     DMVRKHLNEVKSILEKGYEDAQKAFADNANKEALLLDARQKLERQYVIEENSAKMVLS
                     RAIDNAIGTWRKDNPKNIAVLSSGVVLAHNESADITKEVMALVEKENLVFNQLPKVEV
                     HNKNEEKPEQSK"
     gene            complement(9825..9899)
                     /locus_tag="ACHKFK_004150"
     tRNA            complement(9825..9899)
                     /locus_tag="ACHKFK_004150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9864..9866),aa:Arg,seq:cct)
     gene            10095..>10905
                     /locus_tag="ACHKFK_004151"
     CDS             10095..>10905
                     /locus_tag="ACHKFK_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9054374.1"
                     /translation="MALNDTKLRGLYGKPYSGPAEITDGDGLSVRITPAGTITFQYRY
                     RWNGKPVRLTVGRYPSTSLKDARVIVGEMRALYMKGVNPKNYFAPSDGELTLKECLDQ
                     WWDKYVTDLKPNTQTLYRSVVYNTMYTQFEGSPVASIPVSAWARFFDKQESLNKKKAR
                     VLLLQLRSVINWCISRQLIPSCELLKLSVKNIGKKPDVGSRVLTYTELAKIWLALENS
                     KVFTSNKVLHQLLLLWGARLSELRLATASEFNMEDLVWTTPKEHSKMGNIIR"
BASE COUNT         3021 a         2138 c         2922 g         2824 t
ORIGIN      
        1 cataaagagg taggtttgtc ttctttttaa tctttggtgt ataaagccaa aatgtaaata
       61 acaatcaaaa ttatattctg aattaaaata aatacgcagg ttttttacct gcgtttttgc
      121 tatttatagt taattaaatt gatgttgtca aaatgggtca ttttgtaaac ctgtgcctca
      181 aggtttgtct ccccccccgg tgtgcaaagc aaaaggaaat tgggaaaggg gataacagga
      241 aattgattgc atggttttga ttacgacgac aaaaaatatt tttataattc aagcaattaa
      301 gagtatgcgt tggcggggtt tttcgtacgt gaaagtcatc gctgtggacg actattcttc
      361 tctgattcgt gattttggca catcttgctt tcgggatgag caatcgttgt tgttacctgt
      421 attccctgat aatgattcta ctacctgtat acgctcactg tatttcattc accaatggct
      481 aaaaagccag cctgtcaata ttccgtgcct ggcatggggg gataatggtg tagttagtaa
      541 taaaggcata cctttcatac cgtggaaatt aacagcagag caattttgtt tctatataga
      601 taaatttatc aatggctgga ggtgcaaaaa aaatattcgc gcgaatcata aagtttcttt
      661 tttagaacga caattatccc cgagggagaa aagtatcttt atatatacca aggaaggaaa
      721 agatattgca tggatctcga acaccttggg tatatccagt aagactgtgt ggacgcatcg
      781 tcgtagagct atggataaac tcggggtaaa acgtttacat caatttataa atattccgcg
      841 ttcccttatt agtgaaaata ttcaattata atgtacgttc aagagtatta gtgttatgaa
      901 taaaatatat aaagtcattt ggaaccatac aacccaaaaa tgggatgttg tttcagaatt
      961 gacgagttgt agaaaaaaat gtaaatcaac tcgcttagga attgctttat cagcaatggt
     1021 tctggggggc gctattgcaa ttaattgcaa taacgcaatg gcagatgtca tattgtcacc
     1081 agactggcgt ccgggtacga ataactcggg tgttggggct gcaacagtaa gtggtaaaac
     1141 cgaatacatc actggtccaa atgtcgtcca gtctggtggg tcaggtctta tctggatgac
     1201 tgtagaacag gcaattttaa atggctacac cactggagat aatttatccg gattgattta
     1261 cgtcaatacc ggagaaaaaa caaaaaccat tacagtgaag gatgaggtta ctggcgcttc
     1321 tcaaacactt caagtgtttg atactgactc tttctcacaa cgcgatgcgg ggactggggg
     1381 aaatgaaacc attcctggtt ttagtggcac tgcggatttt ttcaatgcga cacgttttgt
     1441 aacagccaat aatggcggta cagctatttt ggatgtgggt tcaccagcaa tcggtaattt
     1501 ttttaaaaat acacagcttg ctgtagctga tggagaaggt tcctctgttg tatggaactc
     1561 ggtcaatgat ttttattttc agcctggtgc aaccatgcag gggggcggcg ttactcaaaa
     1621 aatcattgac tcaatgaaat atgctggaac aattactgat tgggcgggaa aagtacacca
     1681 tattaactct cttgatgatt taaagcaata taatcaatat ttgataaagt cactagagga
     1741 caaaacgctt tcttataagc agtatgatgc tgaatttaat aaggccctta ttgtcaccaa
     1801 gcataattac aatgtggata tgaccgctgg gggacgtata gactcaactc cttacaaaga
     1861 aaatgtaggg ctgcttgctg ttctccatgc aaccaataac gcacgagcaa tattaggtaa
     1921 aacgggtaaa ttaactggag ttcttcctgc ctatgggaat ggaggaggga tcgttgcaac
     1981 taatggtggg accggtgtta atgaaggggt cattgatgcc attggtactg aaatgattgc
     2041 ctatcaagac agtaccattg ttaacgatgg tacacttttt gtttgggata ataatgataa
     2101 atatgctctc caggcagagg ggatggttgc cggtagtaat ggttcttcag ccattaataa
     2161 tggtgttatt aatattcgcc catttaaaaa tgctttcgct ccagagggga ttaacaccgc
     2221 aattgttgtt agtaatgggg gcatggcaac aaataaaggc acaataaata ttactgccga
     2281 tgcatcaact aatgataata atggcaaaac acgaggtgta aatgttggag ctggaggctc
     2341 ttttattaac tcggccttcg ggagcatcaa tgtaggtatt gcggaggata aaactgcgac
     2401 tcattcggct gtgggttctg ttgcgattga agtacaaaat ggtgcaaaca aagtcgttaa
     2461 tgaaggtact atttttttgg gcaggggggc tcaggggaac tacggaatcc tggcaaagga
     2521 tgccgggact gttgatgtgg taaataaagg gactatcact attgacggtc atgacagtga
     2581 tgcacctgca ctgaatgttg gcatgctggc aaataatagc tccgggatga aaaactccgg
     2641 gattatcaat gttaatggtc tgaacagcac cgggctacag gtaatcaatg caggacagtt
     2701 gaattctgac ggtacgataa atgttggcgg caagggtatc agtagtggtt tccgtaacta
     2761 tggtgcctgg gtggaaggtg ccggaagcaa tgttaatgta tccggaaaaa tcagtcttgc
     2821 cggtacgggg gctgtggggg tttttgctaa agatggcggc agtctgaccc tgtcaggcaa
     2881 tggtgccgtg ctatttggca gcagcgatca gataggcttt tatgtctatg gaaaggactc
     2941 tgccattcat aataccggaa gcggtgttat ggatgtgtcc actgaaaact caacattatt
     3001 ccgtattgcc agcggtgcga cattccaggg aactgcagat gcttcttctg cacttacggc
     3061 gtctggtaag aactcttatg cacttattgc cacggggaaa tcggatggcg gtgtggcctc
     3121 gacagtaacg tctggaggaa tgaccatcaa cctgacgggt gagggggcta cagcgacttt
     3181 aattgaaggg ggagcgcagg gcacaattga aagtaatgcc attatcaata tggataatgc
     3241 cagtgcgata gccggtattg cggatggcaa tggctatgat atttccggca aactcattaa
     3301 tccgaaggac aagaccacac tattaacggc gggggctcag ttaagttcca cccaggataa
     3361 agtgaccggg tatatcgccc gtaatggggc cactctaaat aataccggta atatcatctt
     3421 tactggaaag aatacagtgg gcgtccgggt tgaggagggg gctgttggta ccaacagcgg
     3481 aaatattaca gttcaggatg gtggtgtggg actaattgct aatgccacac aagatgttac
     3541 aacgattaat aactcgggaa atctcgttct caagggagga gataatgcta accgtacaac
     3601 gggtataaaa gcatctggta caacaacaac ggttaatatg accgcgggta ctatatcttt
     3661 gcagggacag ggggcgattg gtgttgaggc cagcaataaa ggaactgtta accttgatgg
     3721 ttcggcagta ccgaactttg ctgctgacgg ctctggtatt accgatcaga ttgcttttcg
     3781 tattatcgga gatggtgcaa ccattaagac gaatattgca ccgggaactc tgctggatgc
     3841 cagtggagaa cgttctgtac ttttccgtat tgaagatggg gcaaaacagg ccggctctct
     3901 gctgatgaaa acctctggga caggcagccg tggtatctgg gccacaggga aagggagcaa
     3961 tgtcctggct gatgctggca gtgatttcca gatcctgggc gctcaggctc agggattata
     4021 tgtaactggt ggtgcgacag cgacgctgaa acagggggca tcagttaacc ttgtagggga
     4081 tggcgctgtt gtcgcggaag ttgacggaaa tgaatacgct ctggatggca gtattacaca
     4141 aacgaatact ggctcggtca ttaccaatga ggcagatatc tcttcgccgc tgaataatgc
     4201 caggggcttt attacgcgta atcagggact gttgattaac aacggcaaca ttgatttcac
     4261 tgccggtacc gataatatcg gcgtctgggt tgataacggc cgctttgaaa atacaggaag
     4321 ccgtattgcg gtcaatggcg ttgcattatt tgttgaaggt gcacagtctc agattaccag
     4381 cacaggaggg gatatcgtcg ctgtggatgg tgaggctgcc attaagctgg gggcgggcgc
     4441 gtcactgaac ctggcaggga gtggcttggg tacgatcgaa ggtcagaaaa atgcgcatgg
     4501 catcctgctg gatacagggg ctgtggggct ggttattgat ggtgcgaaaa tcaatgttaa
     4561 tgctgcaggt gcggtcggtc acgggattga gaaccgcgca gaaattgaag gcattcagtt
     4621 aaccaatacg actgaaatta atgtggctga tggcattggt gtacgtactt ctgcctccct
     4681 ggccaagacc aacagcggca ctattaatgt agacggcagt ggaattgcac tggcgttcca
     4741 gaaagctgac ggaagtgaaa ccgataataa cctggatatg tctgactccg ctggattggt
     4801 cattaacctg aagggtacgg acggcacggg tattttcgcc aacactaaag atggtgctgt
     4861 cgtgaaaagc ggtgcaagtg tcaatgttac acaggccgat ggcggttccg ctctggtggt
     4921 taacaatgca gccagtgaag tggttcagag cggtaatctc atctctgctt ctctgagtca
     4981 tgccgtagtg gatgcttcaa aagcacaatc ctttaccaat aaaggtcaga ttaaagctgc
     5041 gtccaccacc gggactgcaa tggcgtttga tgacgccgtg aataccaccg tactgaatga
     5101 cagcggtgct gaaattcagg gggttgtggt tctgaacggc ggtgataaca cattcaccaa
     5161 taaaggcagt attaccggaa ccgtcagtgc gaaagagggt aacaatacat ttttatttga
     5221 tgatggcagc acactgacag gagaagtgac tgcaggaaat ggcaataata atgtaacact
     5281 caatggtaag gctcatgttg atcaggttac tgccggtacc gggaagaaca ccttcaccat
     5341 taaaggcgaa ggggcaacct ggaacctgct ggatggcggg cagggagatt ctgattccct
     5401 gatttttgat aacgccattc atacgctgga ttctgttgta aaactacaga attttgaaca
     5461 tgtcgggctg aagaacagtt cacttgtcac tctgaaggaa gctcttgtgc tgaccgatgg
     5521 ggggaacggt ccgggttccg tcgatattga atcgggcagc gaactggcca ttattcccgc
     5581 agttgcaggc aactttacct ttgatccact gttaacaggc aaaggaacac tgtctgcccg
     5641 tcttgatgcc gacacatctg cttttgaatt cagccataac gtcggggatc aatttgccgg
     5701 aactctgaag ctgggtacca gtagctttgc tctggaaggg ctgaatacga gcgggttaac
     5761 ccatgcaatg ctgatgtctg aaaccgggaa tatcacaacg gttggctccg gtgttcagca
     5821 gattggcggt cttgggttca atggcggaac gctgattttt ggttccgtta tgccgggcga
     5881 taccattgcc agcaacagta ttgaaacctc tgctgcaggt acgctggata tccgggggaa
     5941 aggcacaatt caggtcacca tgccagatga agtgattaat gatattccgg ctgttgatac
     6001 ccgtaagaat ttgctggagc aggatgatgc gcagaccctg gtcacgctgg tgaatgcagc
     6061 gggtaccgtc accggtactg gcgggcaact gcaactggtg gatgaaaacg ggcaggctat
     6121 ttctcacagt cagacgtttg atgtcactca gggcggtgaa gttgtagctc agggaaatta
     6181 tgactataag ctgctgggaa gctccgacgg tattaaaggt gacggactgt acataggcta
     6241 tgggctgaag tcgctggatt tacagggaac cggtgataaa gcgctggtgc tgacaccgag
     6301 agcgaatgcc cagggactgc agacagatct tggcgcacag ttaacggggg caggggatct
     6361 ggccatcgaa gctgcggggc aggttgtcac actgtctaac ggcggtaata actacaccgg
     6421 ggatacgctg gtgcgcagcg gcacattaca gatggcaaat gataatgtac ttggcgcaac
     6481 aggtaatctg aacgtcgcca gcaatggcgt cttcagaaca aacggataca gccagactgt
     6541 cggtgcactg cagacagaaa cgggagcgca cattcagctt gatagcggta gtgtactaac
     6601 ggtcagcggc acgcagcgtc agccggggga tgataatggt ggcatcattg aaaacaacgt
     6661 gctgtccggt gagggaacgc tggctgtcac gggcagcaat ctgacggtac atggcaccaa
     6721 tatcgggttt accggtaatg ccagccttac ccagggggcg ctggttgaaa tgaacggcgc
     6781 acaggggctg ggaagccagg gctccatcag ctttgaaagc cttaatgatc gcctggcgat
     6841 tgatatcgcc gacggtagcg gagtcagcag taacctgagt aaatcgcttt ccggagaagg
     6901 ttcagtgggt atactgaaca ccactgatct gacgctttcc ggggataaca gtaatttcag
     6961 cggtgaattc cgtgtgcaga aagatgctgc tctgcgggct tcagatgaaa aacacctggg
     7021 caccgggctg attgacagtg atggtgtcac ctggcttacc gccagtggaa actggttact
     7081 gaagaatgat attactggct ccggcgctct ggtgaaacag ggggcgggta atctgatcat
     7141 taatcatgag ctgacttata ccggtgatac cacggttgag agtggcgttc tgattgtcgg
     7201 tgatgattca gtcacccgtg ccgctggcgc gacgttgtca ggatccaaaa acattcatgt
     7261 tcttaatggc ggcatactga gtgggctggg gaccgtttcg ggtcaggtta ataaccaggg
     7321 aacactggca tcactgaatg ctctctcagg ttatgaaacg gcagaggtgg gcaattttac
     7381 cgttggctca ctgaccaata ccggcgtcat tcgtctggcc ggtggtaaga caggaaatac
     7441 cctgaccgta aatggcgatt acaccggtgg cggtacgtta attatcaata ccgtgctggg
     7501 ggatgatact tctgccactg acaaactgat tgtcacgggt aacacctccg gtgataccgg
     7561 cgtggtggtg aataatgtcc ggggccaggg ggctcagacc gctgacggca ttgagattgt
     7621 gcatgtcggt gggcagtctg acggtaactt cagactgcag aaccgtgcgg tggcaggggc
     7681 gtgggaatat ttcctgcata aaggtaatgc gggtggtact gacggtaact ggtatctgcg
     7741 ttctgaattg ccgccagaac cacaaccgca gccgcagcca caaccgcagc cacagccaca
     7801 ccccacgccg gataaacctg tacagaaggt ctatcgtccg gaagcgggct cttatattgc
     7861 gaacatagcg gcagccaaca cgctgttcaa catcagaatg cacgatcgtg agggggaaac
     7921 ttactatacc gatgtgttta ctggtgagaa gaaagcaaca agtatgtgga tgcgtcatat
     7981 cggcggtcat aatcgctgga aggacagcag ttcacagctg aatacgcaaa gtaaccgtta
     8041 tgttgtgcaa ctgggtggca gcattgcgca atggactgac ggtcaggacc gtctgcaact
     8101 ggggattatg gccggttatg gaaatgagaa gagttccacg acatcctcgc tttcgggata
     8161 taaatctaaa ggtgcgatca atggatacag caccggttta tacggaacgt ggcagcagaa
     8221 tgacggtaat gacaacgggg cttatgtcga tacctggatc cagtacggat ggtttaataa
     8281 taccgttaat ggtgaaaaac tggctgcgga gtcctggaaa tcccgtggat ttaccggttc
     8341 agtggaagcg ggttatacct ttaaagccgg cgaatttacc ggtagccagg gtagtcacta
     8401 tgactggtat attcagccgc agtcccagat cacgtggatg aatgtaagag ccagtgagca
     8461 tacggagaag aatggcacga aagttcagct cagcggagac ggtaatatcc agagtcgtct
     8521 gggcgtcaga acttacctga aaggtaagag tgcgtctgat gataataagg cgcatcagtt
     8581 tgagccattt gtggaagtta actggatcca taatacacgg agctggggtg tcaaaatgga
     8641 taatacggca ctcagtcagg acggagctac aaatattgct gaagtcaaaa cgggtgttca
     8701 gggtaaattg tcagataacc tgaatgtctg gggcaatgtt ggtgttcagg ctggcgataa
     8761 agggtacagt gatgcccagg caatgctggg tattaaatat atattctaat cacacagtgg
     8821 caggagggga cctcctgcca cctcagggtt gtcataatga gttctgtaat gaaattttct
     8881 gttgttaatg cagtagtgct gctgttgctg attgtactta ctggtgtttt ttatatgaaa
     8941 aagtcaaaag aaattgcagt tgttaatatc gatgctattt ataatcagtc tgccgccgga
     9001 gacatggtca gaaagcatct gaatgaggtt aagagtattc ttgaaaaagg ctatgaggat
     9061 gcgcaaaaag cattcgcaga taacgcgaat aaagaggcgc tgcttcttga tgccagacaa
     9121 aaactggagc gacaatatgt tattgaggaa aatagcgcaa aaatggtgtt atcccgtgcg
     9181 atagataatg ccataggtac atggagaaaa gacaatccta aaaatattgc tgtattgtct
     9241 tcaggtgttg tgcttgcgca taacgagtca gctgatatca ccaaagaagt catggctctg
     9301 gttgaaaaag aaaatctggt ttttaatcag ttacccaaag tggaagtgca caataagaat
     9361 gaagagaaac cagaacaaag taagtaatca ctagattctg acgatttcca gaagggagat
     9421 tcagcaggcc agcatctgta atcattcaga tttgctggct tgctgctaaa tatggttgct
     9481 aagaaaaata gcaggccagt aatttattca gattactgaa tataataatt tttcaggaac
     9541 ggttatgctg aaggtagtat gtttaatcga cacaattatt tgctgtgtct aatatgaggt
     9601 gacattttta tgatcactga ccagcaagac tgacccgtat tgattagcct gattattggc
     9661 aacttattgg ggtagtcatt ttgacatcga atgggggagg tttccagtta agttcacgaa
     9721 tgcatgatga tttgcgttca catatcgaag tactggttga ctatactaag gattttaaga
     9781 taactcactg attgtaagtg ggttattgcg agggggatga tagatggtgt cccctgcagg
     9841 aatcgaacct gcaattagcc cttaggaggg gctcgttata tccatttaac taagaggaca
     9901 ttgttgcgta ttgattttgg ttgttcacca tatgactgat aataccgtct ttatggtttt
     9961 aaaatcaagg tgtaagttcg tttttgttca tggttgatta tcttcttttg cgttgaaaag
    10021 tgttttgttc acttgctaat gcgtacatat tgagtacaga atacattata aacttgtgta
    10081 caggatataa agcattggca ctgaacgaca ccaaactacg aggcctctac ggaaaaccct
    10141 actctggccc tgctgaaatc accgatggtg acgggttaag tgtgcggatt actccagcag
    10201 gaactatcac gtttcagtac cgttatcgct ggaatggtaa gccagtacgt cttactgtcg
    10261 ggcgctatcc gtctacttca ctgaaggacg cgcgtgttat cgtcggtgag atgcgcgcat
    10321 tgtacatgaa gggagttaac cctaaaaatt attttgcgcc cagtgacggc gaactgacat
    10381 taaaagaatg tctggatcag tggtgggata agtatgttac tgatctgaaa cccaatacac
    10441 agacgcttta cagatccgtc gtgtacaaca ctatgtacac acagtttgaa ggctcgccag
    10501 ttgccagtat cccagtatcg gcatgggctc gtttctttga caaacaagaa agcctcaaca
    10561 agaaaaaggc tcgtgttttg ttgttacaac tgcgttcagt tattaactgg tgtatcagcc
    10621 gccagttaat cccatcatgc gaattactca agcttagtgt taagaatatt gggaaaaaac
    10681 cagatgtagg gagtcgtgta cttacctata cggaactggc aaaaatttgg ctggcgctgg
    10741 agaactcaaa agtatttact tccaacaagg tgttacatca attgctattg ctatgggggg
    10801 ccagactttc tgagctacgt ctcgcgacag ccagtgagtt caacatggaa gatctggtct
    10861 ggacaacccc aaaagagcat tcaaaaatgg ggaatattat ccggc
//
LOCUS       DAZGJT010000131        10880 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZGJT010000131 DAZGJT010000000
VERSION     DAZGJT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10880
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.131"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            155..1543
                     /gene="narU"
                     /locus_tag="ACHKFK_004152"
     CDS             155..1543
                     /gene="narU"
                     /locus_tag="ACHKFK_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO9054375.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKNIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1638..5378
                     /gene="narZ"
                     /locus_tag="ACHKFK_004153"
     CDS             1638..5378
                     /gene="narZ"
                     /locus_tag="ACHKFK_004153"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO9054376.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKSLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMVRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLADGSTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVT
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLSLKAEADKAGLSPAEF
                     TVQALKSGELRMACEQPDNGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVEHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5375..6919
                     /gene="narH"
                     /locus_tag="ACHKFK_004154"
     CDS             5375..6919
                     /gene="narH"
                     /locus_tag="ACHKFK_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO9054377.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQDEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIDAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6919..7614
                     /gene="narW"
                     /locus_tag="ACHKFK_004155"
     CDS             6919..7614
                     /gene="narW"
                     /locus_tag="ACHKFK_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO9054378.1"
                     /translation="MQILKVIGLLMEYPDELLWECKDDALALIRRDAPMLTDFALDLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLNCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     PLSSDSVTKQIRSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7611..8291
                     /gene="narI"
                     /locus_tag="ACHKFK_004156"
     CDS             7611..8291
                     /gene="narI"
                     /locus_tag="ACHKFK_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO9054379.1"
                     /translation="MIQYLNVFFYDIYPYLCGTFFILGSWLRYDYGQYTWRAASSQML
                     DKRGMVLWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAVKQQMAMILGGICGVLT
                     LIGGAGLLVRRLTNPRIRATSSGADILILAILLIQCILGLTTIPFSAQHPDGSEMMKL
                     VGWAQSIVTFQGGASTHLDGVAFIFRVHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8371..9264
                     /locus_tag="ACHKFK_004157"
     CDS             8371..9264
                     /locus_tag="ACHKFK_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO9054380.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTVWQTSLAGK
                     HRVTIEKQNDDYRISLEQGTPGFEPPLEGETRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLRVKGHQGRALGRDGVIEVTVTIHDNQP
                     EKVTISGAAVILFHAEWAIEL"
     gene            complement(9360..10205)
                     /gene="nhoA"
                     /locus_tag="ACHKFK_004158"
     CDS             complement(9360..10205)
                     /gene="nhoA"
                     /locus_tag="ACHKFK_004158"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO9054381.1"
                     /translation="MTPILNHYFARINWLGAAAANIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEDKLVITRRGGYCFEQNGVFERVLRELGFNDRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLMPDIMQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10380..>10880
                     /locus_tag="ACHKFK_004159"
     CDS             10380..>10880
                     /locus_tag="ACHKFK_004159"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO9054382.1"
                     /translation="MSQFIPVELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVRGPVLGLPLVEEKCLAWMECRLLPATSAQEKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDK"
BASE COUNT         2548 a         2870 c         2875 g         2587 t
ORIGIN      
        1 ttaatccaca ttctgccccc gcagttacgc tttcttatct attcttctaa tcagcacttc
       61 acctgattca tccttaattc taataactgg ctattgcgcc ttacctcctg ttttgtcttt
      121 ttttcattac caatgtgtgc atgtgaggaa caatatggca ctgcaaaatg agaaaaatag
      181 tcgttatctt ttacgcgact ggaaaccaga aaatccggcc ttctgggaaa ataaaggaaa
      241 gaatattgca cgaagaaacc tctggatatc agtcagttgc ctacttcttg ccttctgtgt
      301 ctggatgcta tttagcgcag ttactgttaa tctcaataaa atcggtttta atttcactac
      361 cgatcaactc tttttattaa ccgcattacc ctccgtttct ggcgcattat tgcgtgtccc
      421 ctactccttt atggtgccta tattcggtgg acgccgatgg acggttttta gtactgcaat
      481 cctgattatt ccttgcgtct ggctcggaat tgccgtgcag aatccgaata ctccttttgg
      541 gatatttatc gttatcgctt tgctatgcgg ttttgcaggt gcgaactttg cttcgagcat
      601 gggcaatatc agtttcttct ttccaaaagc caaacaaggg agcgctcttg ggattaatgg
      661 cggattagga aacttaggtg taagtgtgat gcagctggtt gcaccgctgg tcatttttgt
      721 ccccgtattt gcctttctcg gcgtcaatgg cgtaccgcag gccgacggtt cggtgatgtc
      781 gctggcgaat gccgcatgga tttgggtgcc attactggcg attgccacga tcgccgcctg
      841 gtcagggatg aatgatatcg ccagttcacg cgcctcaatt gccgaccagc tccctgtctt
      901 acaacgcctg catctctggc tgctgagcct gctttatctt gccaccttcg gttcgtttat
      961 cggtttttct gcgggttttg ccatgctggc aaaaacccag ttcccggatg tgaatattct
     1021 gcgcctggcg ttctttggcc catttatcgg tgccatcgcg cgatcggttg gtggtgctat
     1081 ttccgataaa ttcggcggcg tgcgggtgac gttgatcaac ttcattttta tggcgatttt
     1141 cagcgccctg ctgttcctta ccttaccggg cacaggctcc ggtaatttca tcgcatttta
     1201 cgccgtattt atggggctgt ttctgaccgc gggtctggga agtggttcta ctttccagat
     1261 gatcgccgtc atctttcgcc agataacaat ttatcgggtg aagatgaaag gcggtagtga
     1321 tgagcaagct caaagagaag ccgtcaccga aacggcagcc gctctgggct ttatctcagc
     1381 cattggcgca gtgggcggct tttttattcc gcaggcgttt ggcatgtcgc tcaatatgac
     1441 cggctctccg gtgggcgcga tgaaagtgtt tttaatcttc tacatcgttt gtgtgctact
     1501 gacctggctg gtttatggtc ggcggaagtt cagccaaaaa taagagatcg atgcctgaca
     1561 cgtcttatca ggctacaacc gtgtctcatc cgacaaggca tcagatccca attcattcct
     1621 gaagcaggag ttatgtcatg agtaaacttt tggatcgctt tcgctacttc aaacaaaagg
     1681 gcgaaacctt tgccgatggt cacggacagg tgatgcatag caaccgcgac tgggaggaca
     1741 gctatcgcca gcgttggcag ttcgacaaaa tcgtgcgttc cacccacggt gttaactgta
     1801 caggctcctg cagctggaaa atctacgtta aaaatggtct ggtgacctgg gaaatccaac
     1861 agaccgacta cccgcgcact cgccctgacc tgccaaatca tgaacctcgc ggctgcccgc
     1921 gtggcgcaag ttactcctgg tatctctaca gcgctaaccg cctgaaatac ccgctcattc
     1981 gtaaacgact gattgaactg tggcgcgaag ccctcaaaca acacagcgat ccggtactgg
     2041 cgtgggcatc gattatgaac gatccgcaaa agagcctgag ctacaaacaa gtgcgtggac
     2101 gcggtggctt tatccgctcc aactggcagg agctaaatca gctgattgcc gccgctaacg
     2161 tctggaccat caaaacttac ggcccggatc gcgttgccgg tttctcgccg attccggcga
     2221 tgtcgatggt ttcttacgcc gccggaacac gctatctgtc gttgttaggt ggcacctgtt
     2281 taagcttcta cgactggtat tgcgacctgc cgcccgcctc gccgatgacc tggggcgagc
     2341 aaaccgacgt accggaatct gccgactggt ataactccag ttacatcatc gcctgggggt
     2401 ctaacgtacc gcagacacgt acgccggacg cccatttctt taccgaagta cgctacaaag
     2461 gcactaaaac catcgccatt acccctgact actctgaagt ggccaaattg tgcgaccagt
     2521 ggctggcacc gaaacaaggc actgatagtg ccctggcaat ggcaatgggc catgtcattt
     2581 taaaagagtt tcatctcgat aatcccagcg attattttat caactactgc cgccgctaca
     2641 gcgacatgcc gatgctggta atgctggaac ctcgcgacga cggcagctac gttcccgggc
     2701 gcatggtccg cgcatctgac ctggtggatg gactgggcga aagcaataat ccgcagtgga
     2761 aaaccgtggc agttaatacc gcaggtgaat tggtagtgcc gaacggttcg attggttttc
     2821 gctggggaga aaaaggcaaa tggaacctgg aatccattgc cgccggtacg gaaaccgaat
     2881 tgtcgttaac cctgctcggt caacatgacg ccgttgcagg cgtggccttc ccctactttg
     2941 gcggcattga aaatccacat tttcgcagcg taaaacacaa tcctgtcctg gtgcgccaat
     3001 tgcccgttaa aaacctgaca ttagccgatg gtagcacctg tccggtggtc agcgtttatg
     3061 atttggtact ggcgaattac ggcctcgatc gcgggctgga agatgaaaac agcgcgaaag
     3121 attacgctga aatcaaacct tatacccctg cctggggcga gcaaattacc ggcgttccgc
     3181 gccagtatat tgaaaccatc gcccgtgaat ttgccgatac cgcccataaa acgcatgggc
     3241 gctcgatgat tattctcggc gcaggtgtta accactggta tcacatggac atgaactacc
     3301 gcgggatgat caatatgctg atcttctgcg gttgtgtcgg gcaaagcggt ggcggctggg
     3361 cacactatgt cggtcaggaa aaactgcgcc cgcaaaccgg ctggctgcct ctggcctttg
     3421 cgctcgactg gaaccgacca ccgcgccaga tgaacagcac ctcgtttttc tacaatcact
     3481 ccagccagtg gcgctatgaa aaagtcaccg cgcaggaatt gctctcaccg ctcgccgatg
     3541 ccagtaaata cagcggtcac ctgattgatt tcaacgttcg cgccgaacgt atgggctggc
     3601 taccttctgc gccgcagctg gggcgtaacc cgctctccct gaaagccgaa gccgacaagg
     3661 ccggattgtc acccgccgaa tttaccgtcc aggcactgaa gtcaggcgaa ttacgcatgg
     3721 cctgcgaaca gccggacaac ggcagcaacc atccgcgtaa cctgtttgtc tggcgttcaa
     3781 acctgcttgg ctcttccggc aagggccacg agtatatgca gaagtatctg ctggggaccg
     3841 aaagcgggat tcagggcgag gaactcggtg ccagcgacgg gatcaaacca gaagaagtcg
     3901 agtggcaaac tgcggcgatt gaaggcaagc tcgacctgct ggtgacgctc gacttccgca
     3961 tgtccagtac ctgtctgttc tccgatatcg ttctgcccac cgccacctgg tacgaaaaag
     4021 acgatatgaa tacctcggat atgcatccat ttattcatcc gctttctgcg gcggtcgatc
     4081 cggcctggga atcacgcagc gactgggaaa tctacaaagg tatcgctaaa gccttttcgc
     4141 aagtgtgcgt ggagcatctc ggcaaagaaa ccgacgtggt attacaacca ctgctgcatg
     4201 actccccggc agagctttca cagccgtgtg aagtgctcga ctggcgcaaa ggtgaatgcg
     4261 atctgattcc aggcaaaacc gcgccgaata ttgtggcggt ggagcgcgac taccctgcta
     4321 cctatgaacg ctttacctca ctcgggccat tgatggacaa acttggcaac ggcggtaaag
     4381 ggatttcgtg gaatacccag gatgaaatcg atttcctcgg taaactcaat tacaccaagc
     4441 gtgatggccc ggcacagggg cgtccgctga ttgataccgc gattgacgca tcagaagtga
     4501 ttctggcgct ggcaccggaa accaacggcc atgttgcagt caaagcgtgg caagcgctgg
     4561 gcgagatcac cggacgcgaa catacccatc tggcgctgca caaagaggac gagaagattc
     4621 gctttcgcga tattcaggcg cagccgcgta aaattatctc cagccccaca tggtctggtc
     4681 tggaaagcga tcacgtttcc tataacgcgg gatacaccaa cgttcatgag ctaattccgt
     4741 ggcgcacact gtcgggacgc cagcaactct atcaggatca tccgtggatg cgtgcttttg
     4801 gtgaaagtct ggtggcttat cgcccgccta tcgacacccg tagcgtcagt gaaatgcgcc
     4861 agataccgcc aaacggcttc ccggaaaaag cccttaactt cctgacgccg caccagaaat
     4921 ggggcattca ctcaacctac agtgaaaacc tgctaatgct gacgctctct cgcggtggac
     4981 cgattgtctg gatcagcgaa accgatgccc gagaactgac cattgtcgat aacgactggg
     5041 tggaagtttt taacgccaac ggcgcgctga cggcccgcgc ggtggtcagc caacgtgtac
     5101 cgccgggcat gaccatgatg tatcacgccc aggaacgcat tatgaatatt cctggttcgg
     5161 aagtaactgg catgcgcggc ggcattcata actcggttac ccgcgtttgc ccgaaaccaa
     5221 cgcatatgat tggcggttac gcgcagctgg cctggggctt taactactac ggcaccgtcg
     5281 gctcgaaccg cgacgaattc attatgatcc gcaaaatgaa gaacgttaac tggctggatg
     5341 atgaaggtcg cgatcaggtt caggaggcga aaaaatgaaa atacgttcgc aagtcggcat
     5401 ggtgcttaac ctcgataaat gtatcggctg ccatacctgt tcggtgacct gtaaaaacgt
     5461 ctggaccgga cgcgaaggca tggagtacgc atggtttaac aacgtcgaaa ccaaaccggg
     5521 cattggttat ccgaaaaact gggaagatca ggatgagtgg caaggtggct gggtgcgtga
     5581 tgtgaatggc aagatacgcc cgcgtctggg cagcaagatg ggcgtaataa ccaaaatttt
     5641 cgccaacccg gtagtgccgc agattgacga ttactacgaa cctttcacct tcgactacga
     5701 acatttgcat agcgcgccgg aaggcaaaca tattcccacc gcccgtccgc gttcactgat
     5761 tgacggcaaa cggatggaca aagtgatctg gggcccaaac tgggaagaac tgctgggcgg
     5821 cgaatttgaa aaacgagccc gcgaccgcaa cttcgaggcc atgcaaaagg agatgtacgg
     5881 gcagtttgaa aacaccttca tgatgtatct gccgcgcttg tgcgaacact gccttaaccc
     5941 cagttgtgta gcgacctgcc ccagcggcgc tatctacaaa cgcgaagaag acggcattgt
     6001 gctgatcgat caggataaat gccgtggctg gcgtttgtgc attagcggtt gcccatacaa
     6061 aaaaatctac ttcaactgga aaagcggcaa gtcagaaaaa tgtatatttt gttatccgcg
     6121 aattgagtcc ggtcaaccga ccgtgtgctc agaaacctgc gtgggtcgca tccggtattt
     6181 gggcgtgcta ctttacgacg ccgaccgcat cgaggaagcg gcgagcaccg agcgcgaagt
     6241 tgacctctat gaacgccagt gcgaagtgtt cctcgatccg cacgatcctt cggtgatcga
     6301 agaagccctg aaacaaggta ttccacaaaa cgtgattgac gctgcccagc gttcgcctgt
     6361 ctacaaaatg gcgatggact ggaaactggc gctaccgctg caccctgaat accgcaccct
     6421 gccgatggtc tggtacgttc cgccgctgtc accgattcag tcctacgctg atgcgggcgg
     6481 cttgccgaaa agcgaaggcg tgctgcccgc catcgaaagc ctgcgtattc cggtgcaata
     6541 tctcgccaat atgttgagtg ccggtgatac cggtccggta ctgcgggcgc tgaaacggat
     6601 gatggcgatg cgccactata tgcgttcaca aaccgtggaa ggcgttactg atactcgtgc
     6661 catcgacgaa gtaggcctga gcgtcgccca ggtcgaagag atgtatcgtt acctcgccat
     6721 tgccaactat gaagatcgtt tcgtcatccc gacaagccat cgggaaatgg cgggcgatgc
     6781 cttcgcagaa cgcaacggct gcggttttac ctttggcgac ggttgccacg gttcggacag
     6841 caaattcaac ctgttcaaca gtagccgtat cgatgccatc aacatcaccg aagtgcgcga
     6901 caaagcggag ggcgaataat gcagatcctc aaagtgatcg gcctgttgat ggagtaccca
     6961 gacgagttgt tgtgggagtg taaggacgac gcgctggcgc ttatccgccg cgacgcgccg
     7021 atgctcaccg attttgcact cgacctgctc aacgccccgc tactggataa acaggccgaa
     7081 tggtgtgaag tgtttgaccg cgggcgcacc acgtcgctgc tgctgtttga acatgttcat
     7141 gccgagtccc gcgatcgcgg ccaggcaatg gtggatctgc tggcggagta tgaaaaggtc
     7201 ggtctgcaac tgaattgtag ggaactgccc gattatctac cgctgtatct ggagtattta
     7261 agcgtacttc cggacgatca ggcgaaagaa ggattactca acgttgcgcc gatcctcgcc
     7321 ctgcttggcg gacgcttaaa acaacgcgag gcaccgtggt acgcgttatt tgacgccctg
     7381 ttgcaactgg caggaagccc tctatcaagt gacagtgtca caaaacagat tcgcagtgaa
     7441 gagcgtgacg acacccgcca ggcgctggat gcggtgtggg aagaagaaca ggtgaagttt
     7501 attgaagata acgccacggc gtgtgacagc tcgccgctta atcaatatca gcgacgcttt
     7561 agccaggatg tcgcgccgca gtatgtcgac atcagtgcgg gaggtgggaa atgattcagt
     7621 atctgaacgt ctttttttac gatatctacc cgtacttgtg tggcacgttt tttatccttg
     7681 gaagctggct gcgctacgat tacgggcagt atacctggcg tgccgcctcc agccagatgc
     7741 tggataaacg cgggatggtg ctatggtcga acttgttcca tatcggcatt ctggggatct
     7801 tcttcggtca tctgttcggc atgttaacgc cgcactggat gtacgcgtgg tttctgccgg
     7861 tggcggtaaa acagcagatg gcgatgatcc tcggcggaat atgcggggta ttgacgctga
     7921 ttggcggtgc ggggctactg gtgcgtcgtt taacaaatcc gcgtatcagg gcgacgtctt
     7981 ctggtgcgga tatcctgatc ctcgccattt tgttaattca gtgcattctt gggttaacca
     8041 ccattccttt ttcagcacaa catccggacg gcagtgaaat gatgaagctg gtgggctggg
     8101 cgcaaagcat cgtcactttc cagggcggtg catctacgca tcttgatggt gtcgccttta
     8161 tattccgcgt tcatctggtg cttggaatga ccatcttcct gctcttcccg ttcacccgac
     8221 tggtgcatgt ctggagcgcg ccgtttgagt actttactcg tcggtatcag attgtgcgtt
     8281 cgcggcgtta attctcacgc gggccggata gcccttcgct ctccggccac ttgcaggcta
     8341 tgatagttct ccagcaacag gagaacatta atgaaaccgc aggtttacca cgtcgatgct
     8401 tttacctctc aaccgtttcg cggcaattct gccggtgttg ttttccccgc cgataatctc
     8461 agtgaagcgc aaatgcagct tatcgcccgc gagttaggtc attcggaaac tgcttttctg
     8521 ctgcacagcg acgacagcga tgtgcgcatc cgctacttta cgccaacggt tgaagttccg
     8581 atttgtggtc acgcgacagt agctgcgcac tatgtacgtg caaaggtgtt aggtttagga
     8641 aattgcacgg tctggcaaac atcgctggca ggaaaacatc gtgtgactat agaaaagcaa
     8701 aatgatgatt atcgtatttc gctggaacaa ggtactccag gctttgagcc accgctggaa
     8761 ggtgaaacac gtgcagcgat tatcaacgcg cttcatctca ctgaggacga tattctgcca
     8821 ggcttgccaa ttcaggtggc aacaacagga cactcaaaag tgatgattcc actgaagccg
     8881 gaagtggata tcgacgccct ttcgcctgac cttgctgcgc tgaccgcaat cagcaagcag
     8941 attggctgca atggtttctt cccgttccag atccgcccag gcaaaaacga aaccgatggt
     9001 cgcatgttct cgcctgcgat tggtattgtg gaagatccgg tgactggcaa tgccaatggc
     9061 ccgatgggtg catggttagt acatcacaac gtattgcccc acgatggcaa cgtgttgcgt
     9121 gttaaaggcc atcaggggcg cgcattaggg cgtgacggcg tgattgaagt gacggtgaca
     9181 attcatgata accaaccgga gaaagtcacc atttctggcg cggcggtgat tttgtttcat
     9241 gccgaatggg caattgaact ctgaaaccgt tgcctgatgc gcttcgctta tcaggtctac
     9301 aaccgggcta atgtaggccg gataaggcgt ttacgccaca tccggcaacc tgacataaat
     9361 tattttcccg cctccgggtg cgtatcaaac gccgccatca ccagcgctaa ctcatccaca
     9421 gtaaagccat gtttcgcatc atctacgccc agaccaaact gttcctgcat cacggcatat
     9481 aacgacgcca catctggtag atttcgctgc tcaactgcgt gcccattttc ataatgggta
     9541 aaatgaaaat tggtcagcgt tagcttgcca ccgtccggca aatggcgaca catcagcaaa
     9601 tgatggcgaa aatgcgactg cggccagtgc gccgaccaga aattgcccat cacataatcg
     9661 ctttgttgct gctcgcagag atcaaaacgg tacatcgact gccagtgctg atgatgatta
     9721 aactgcaata cccaatcatc accctcttgc aacaaccgat actctccgtg tggcgtggtt
     9781 tgcatgatat ccggcattaa acgaatcggc gcggttagcg tctgcccacc gaaaccgaca
     9841 tcagcaatcc atttttcccc ttccagttcc accaacagca aacgatgggt gcgcggcggt
     9901 aatgctggcg gatttgataa cactacgcga cctaacaagc tgcgatcgtt aaaccccagc
     9961 tcgcgtaaca cccgctcaaa cacgccattc tgctcaaaac agtaaccgcc acgacgggtt
    10021 atcaccagtt tatcttccag cgattgatca tcaagctgta tttccctcgg cagcaaaacg
    10081 tcgagatttt caaacggaat ggtgcaattg tgtttcaggt gcaatgcacg cagcgtatca
    10141 atattggccg cagcagctcc caaccagtta atacgggcaa aataatgatt cagaatgggc
    10201 gtcatcaggc gtttccctta gtggttttct caacttataa cctggaatag acgccgcact
    10261 ggctattttg tgcgatcgcg gacatgcatc acgttcgcaa atcgccaatc ccgttaattt
    10321 cgttacttat tgcgttacga gctacgcttt taaaacaccc ttcatagcga ggcgatatcg
    10381 tgagccaatt cattcccgtc gaattacacc atgccagccg tctgttaaat catggcccaa
    10441 ctgtcatgat caccagtttc gatgagcaat cccagcggcg taacattatg gcagcagcct
    10501 ggtcgatgcc cgtagagttt gaaccgccac gcgtggcgat tgtggtagat aaatcgacat
    10561 ggaccaggga gttgattgaa cgtaacggta agtttggcat cgttatcccg ggcgttgcgg
    10621 cgactaactg gacgtgggcg gtgggaagtg tgtcgggtcg tgaagaagat aaatttaatt
    10681 gctacggcat tccggtagtg agaggcccgg tgcttggttt gccgctggtc gaggaaaaat
    10741 gtctggcgtg gatggagtgt cgcctgctac ctgcaacttc cgcgcaagaa aaatacgata
    10801 cgctatttgg cgaagtggta tcagcagcgg cagacgcacg ggtatttgtc gaaggccgct
    10861 ggcagtttga tgatgataag
//
LOCUS       DAZGJT010000132        10870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZGJT010000132 DAZGJT010000000
VERSION     DAZGJT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.132"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..654
                     /gene="evgA"
                     /locus_tag="ACHKFK_004160"
     CDS             40..654
                     /gene="evgA"
                     /locus_tag="ACHKFK_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO9054383.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            659..4252
                     /gene="evgS"
                     /locus_tag="ACHKFK_004161"
     CDS             659..4252
                     /gene="evgS"
                     /locus_tag="ACHKFK_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO9054384.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADGDYIEYRGISSNNRVTLDPLRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRIRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEDGEVDIVLSHLVASPPLNDDIAATNPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFLLTRKDSIVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDETGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPAAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVQNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     ESPFKDVFSNTHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASEHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTLPERYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTIFTITIPVEITQQVAAVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLNCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPTAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQQND"
     gene            complement(4308..5453)
                     /gene="yfdE"
                     /locus_tag="ACHKFK_004162"
     CDS             complement(4308..5453)
                     /gene="yfdE"
                     /locus_tag="ACHKFK_004162"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO9054385.1"
                     /translation="MTNNECKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINSRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLVKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5527..6471)
                     /gene="yfdV"
                     /locus_tag="ACHKFK_004163"
     CDS             complement(5527..6471)
                     /gene="yfdV"
                     /locus_tag="ACHKFK_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO9054386.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6541..8235)
                     /gene="oxc"
                     /locus_tag="ACHKFK_004164"
     CDS             complement(6541..8235)
                     /gene="oxc"
                     /locus_tag="ACHKFK_004164"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO9054387.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQSDEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAAETQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8289..9539)
                     /gene="frc"
                     /locus_tag="ACHKFK_004165"
     CDS             complement(8289..9539)
                     /gene="frc"
                     /locus_tag="ACHKFK_004165"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO9054388.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10051..10683)
                     /locus_tag="ACHKFK_004166"
     CDS             complement(10051..10683)
                     /locus_tag="ACHKFK_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO9054389.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVKQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NVNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3195 a         2467 c         2216 g         2992 t
ORIGIN      
        1 agggagaagg gaaatgcttc attgcaaagg gaataatcta tgaacgcaat aattattgat
       61 gaccatcctc ttgctatcgc agcaattcgt aatttattga tcaaaaacga tattgaaatc
      121 ttagcagagt tgactgaagg cggaagcgcc gttcagcggg tggaaacact taagcctgat
      181 atcgtcatca ttgatgtcga tatccccgga gttaacggta tccaggtgtt agaaacgctg
      241 agaaagcgcc aatatagcgg aattattatt attgtctccg ctaaaaatga ccatttttac
      301 gggaaacatt gtgctgatgc tggcgctaat ggtttcgtga gtaaaaaaga aggcatgaac
      361 aatatcattg cggctattga agctgcaaaa aatggctact gctatttccc cttctctctc
      421 aaccggtttg ttggaagttt aacgtccgac cagcaaaaac tcgactcctt atcgaaacaa
      481 gaaattagtg tcatgcggta tattcttgat ggcaaggata ataatgacat tgctgaaaaa
      541 atgttcatca gcaacaaaac tgtcagcact tataaaagtc gcttgatgga aaaattagaa
      601 tgcaaatcac tgatggatct ttacacattc gcacaacgta acaaaatcgg ctaaccacat
      661 gaagttttta ccctatattt ttcttctctg ttgtggtctt tggtcgacca taagtttcgc
      721 agacggagat tacatcgaat atcgtggcat cagtagtaac aaccgtgtca cacttgatcc
      781 actacgtctg agcaacaagg aattacgttg gctcgcgagc aaaaaaaatc ttgtgattgc
      841 agtacacaag tcccaaacag ctacgttgtt gcataccgat tcgcagcaac ggattcgtgg
      901 tattaatgct gattatttaa atcttttaaa aagagcgtta aatatcaaat taacactccg
      961 ggaatacgca gatcatcaaa aagcaatgga cgcgctggaa gacggtgaag ttgatatagt
     1021 gttatcacat ttagttgctt cgccgcctct taatgatgac attgctgcaa ccaatcctct
     1081 gataattacc tttccggcgc tggtcactac ccttcacgat tcaatgcgac cgcttacctc
     1141 atcaaaacca gtaaatattg ctcgagtagc aaattatccc ccagacgagg taattcatca
     1201 atcatttcca aaagcaacaa ttatctcttt tacaaattta tatcaggcat tagcatccgt
     1261 ctcagccgga cagaatgatt actttattgg tagtaacatc attaccagca gtatgatttc
     1321 ccgttatttt actcactcct taaatgtagt gaagtattat aactcaccgc gtcaatataa
     1381 ctttttatta accagaaaag attcaatcgt tcttaatgaa gtactcaatc gatttgttga
     1441 tgctttaaca aatgaagttc gctatgaagt atcacaaaat tggcttgata caggaaacct
     1501 ggcctttctg aacaaaccat tagaactcac tgaacatgaa aaacagtgga ttaagcagca
     1561 tcccgattta aaggtgctgg aaaatcctta ctcgccaccc tattctatga cagatgaaac
     1621 tggctcagtt cggggcgtga tgggtgatat tcttaatatt attaccttgc aaacaggttt
     1681 aaatttttct ccgatcaccg tttcacacaa tattcatgct gggacacagc ttaaccccgg
     1741 tggatgggat atattgcccg ccgctattta tagtgaagat cgagaaaata atgtttcatt
     1801 tgctgaagcc ttcataacaa cgccttacgt ttttgtcatg caaaaagcgc ctgacagtga
     1861 acaaacatta aaaaaaggaa tgaaagttgc cattccatat tattatgagc ttcattcgca
     1921 attaaaagag atgtatccgg aggtagagtg gataaaagtc gataacgcca gcgctgcatt
     1981 tcacaaggtc aaggaaggtg aacttgatgc tctggtcgcg acacagttaa attcgcgtta
     2041 catgatcgac cattactatc ctaatgaact ttatcatttt cttattcccg gcgttcagaa
     2101 tgcatcactt tcgtttgctt ttcctcgcgg agaaccggaa cttaaggata ttattaataa
     2161 agcactgaat gcaattcccc caagcgaagt tctgcgcctg accgaaaaat ggattaaaat
     2221 gcccaatgtg accattgaca catgggacct ctatagcgag caattttata ttgttacgac
     2281 attatccgtt ttattagttg gcagtagcct tttatgggga ttctacctgt tacgctcagt
     2341 tcgtcgtcgt aaagttattc agggtgattt agaaaaccaa atatcattcc ggaaagcgct
     2401 ctcggattcc ttaccgaatc caacttatgt tgtaaactgg caaggtaatg tcattagtca
     2461 caatagtgca tttgaacatt atttcactgc tgattactat aaaaatgcaa tgttaccatt
     2521 agaaaacagt gaatccccct ttaaagatgt tttttctaat acgcatgaag tcacagcaga
     2581 aacgaaagaa aaccgaacaa tatacacaca ggtatttgaa attgataatg gcatcgagaa
     2641 aagatgcatt aatcactggc ataccttatg taatctgcca gcaagcgaac atgctgttta
     2701 tatttgtggt tggcaagata ttaccgagac gcgtgattta attcatgcac tcgaagtaga
     2761 gagaaataaa gcgatcaatg caactgtcgc aaaaagccag tttctggcaa caatgagtca
     2821 cgaaataaga acaccaataa gctccattat gggcttcctg gaacttctgt cgggttctgg
     2881 tcttagcaag gaacaacggg tggaggcgat ttcacttgcc tacgccaccg gacaatcact
     2941 cctcggctta attggtgaaa tccttgatgt cgacaaaatt gaatcgggta actatcaact
     3001 tcaaccacaa tgggtcgata tccctacttt agtccagaac acttgtcact ctttcggtgc
     3061 gattgctgca agcaaatcga tcgcattaag ttgcagcagt acgcttcctg aacgctacct
     3121 ggttaagatc gaccctcagg catttaagca ggtcttatct aatttactga gtaatgctct
     3181 caaatttact accgaggggg cagtaaaaat tacgacctcc ctgggtcaca ttgatgacaa
     3241 ccacgctgtt atcaaaatga cgattatgga ttctggaagt ggattatcgc aggaagaaca
     3301 acaacaactg tttaaacgct atagccaaac aagtgcaggt cgtcagcaaa caggttctgg
     3361 tttaggctta atgatctgca aagaattaat taaaaacatg cagggcgatt tgtcattaga
     3421 aagtcatcca ggcataggaa cgatatttac gatcacaatc ccggtagaaa ttacccaaca
     3481 agtggcggct gtcgaggcaa aagcagaaca acctataaca ctacctgaaa agttgagcat
     3541 attaatcgcg gatgatcatc cgaccaacag gctattactc aaacgccagc taaatctatt
     3601 aggatatgat gttgatgaag ccactgatgg tgtacaagcg ctacacaaag tcagtatgca
     3661 acattatgat ctgcttatta ctgacgttaa tatgccgaat atggatggtt ttgagttgac
     3721 tcgcaaactc cgtgagcaaa attcttcctt acccatctgg gggcttacag ccaacgcaca
     3781 ggctaacgaa cgtgaaaaag ggttaaattg cggcatgaac ttatgtttgt tcaaaccgtt
     3841 gaccctggat gtactgaaaa cacatttaag tcagttacac caggttgcgc atattgcacc
     3901 tcagtatcgc caccttgata tcgaggccct gaaaaataat acggcgaacg atctacaact
     3961 gatgcaggag attctcatga ctttccagca tgaaacacat aaagatctac ccactgcgtt
     4021 tcatgcacta gaagctggcg acaacagaac tttccatcag tgtattcatc gcatccacgg
     4081 tgcggctaac atcctgaatt tgcaaaagtt gattaatatt agccatcagt tagaaataac
     4141 acctgtttca gatgacagta agcctgaaat tctccagctg ctgaactctg ttaaagaaca
     4201 cattgcagag ctggaccagg agatagctgt tttctgtcaa caaaatgact aaatagcggc
     4261 tcccacaata ttcaaatgtg ggagctattt accagcacat ctcacattta tgatgagaac
     4321 tcctggcgaa tttgttcccc atgctggtcg agcgttgccg ctcccggcat aacatgcgga
     4381 tccgcgcagc cgctgatttt tatcggatta cctggcatca ttattccccc ggcttcaatc
     4441 aacatatttc tcgcctgagt ttgtggcaaa ttaatagcct cagccacact taacaacggc
     4501 gcgacgggca caccaacttc atgtatttta actaaccaaa cttcagctgc ctgcgttttt
     4561 aacgttcgct caatatattg tttaagaata gcctggtttt gtacgcgtaa aatattgctg
     4621 ctaaatcggg gatcattaac cagttccgta agctccagtg cctggcataa cgcagaaaaa
     4681 agcttgtcat taccacaaca aatagtaatc ggcttatcct gagtatcgaa aacatcaaaa
     4741 ggtgccatgt agggatggcg atttcctaaa cgttgtggtg acttcccagt cgcgatatat
     4801 gccatcagtc catgctccag aaaactcaga gtggcatcaa acatcgctat atcgacatgc
     4861 gcacctctct ggctcttttc gcggccataa agtgcactca ctattccact gaataaataa
     4921 acaccaccac agagatccgc aagagaggta ccaacgcgca ctggcggagc atcagggtat
     4981 cctgtttcca tcattatccc gctcattgcc tgaatgatgg tatcgtaggc aggagcatct
     5041 tttagcggac cggtatgtcc gaaacctgac gatgaagcat atataaggcg cgagttgatt
     5101 tcttgtaacc tttcccatga aaacccgagt ttttccattg tacctgggcg aaaattctca
     5161 gctaatacat cagcttgttt aagcatattt ataaatatac ttttatcgtg atcattcttt
     5221 aaatcaagaa ccacactctc tttgccatga ttaataaaac tgtaatagag tgactgtcca
     5281 tccacatagg gaccaaatgt gcgggtatca tcaccatgac ccggcggctc aactttaatt
     5341 acccttgcgc ccatattaca aagaagttga gttccgaaag gcccattaag gacatgcgtc
     5401 atatcgataa ctaataagcc ttcaaacggc cctttgcatt cattatttgt catttgctta
     5461 tgccttgaaa aaagccgaag gtgaaaagac acttcggcat ttatgtaaaa tcaataaaat
     5521 gattaattat gaaaccagtc gactgacata aatccacaaa ggagccgtga cgacaaaacc
     5581 cagtacgctc acagccaatg acgctgtacc agtgcgggtg tagacattaa accggctggc
     5641 aataatgatt ccggagaatg ccggcggtaa tgcgcctgcc agtaccatca tctgcaaatg
     5701 ttcgctattc aaatgacatg ccatacctac gagaagcagc gccagtggca tcagaatcag
     5761 cttcaggaaa gtgttataag caatttcggc actgaactcg aatttatgtg cagccagagt
     5821 caaccccgca gcgaataccg ctacccctga gttagccttc gcaatcagat taaaggttgg
     5881 gtcccatgct gccggaattt ttaccccaac cagcaccagg atcgttgcca gaacaggtgc
     5941 ccatactact ggctcctttg ccgcagaaat taatgcgctc agattactat tcttcttacc
     6001 atccgctcct gaggaaggat tcagcaaata cagaccaata ggaatagtaa ttgcgttaac
     6061 aataatagaa ataattgcta ccactaaacc tgttgatacg gaatcaccat aaataggatc
     6121 gagaactgca aaccccagga atccaatggt aggtgaacct gcaattaatg cacatactgc
     6181 tgcttctgca tgggtacgtt taaaaaattt gtagcaaccg aaccaggaga aaaagaaaca
     6241 cccgacaata accacaagtg atacaagggt caggcgagtg tccgcaaaaa tcatttccct
     6301 gtttgccctg gtaatagaaa caaatagagc cgcaggaaga gcgtagttta ataccagttt
     6361 attaaatgcc cgagcttgat cctctgaaaa agtttctcgt ctgccgctaa aataacccaa
     6421 cagcatgatt acgataatag gcaataaatc gccaataaaa aatgttagca taatagtatt
     6481 tccgataagg gtccccttac catcgcataa taagttggca agaggagacg atgtcagtat
     6541 ttaattacca gcgacttgtt ttgggttaag tttggtaata tggccacttt cagttcctgc
     6601 tgctgggtcg atgaccacat taataatggt cggtttgcgc gactgaattc cggtggttaa
     6661 agcatgacga agttcatctg tcgtggtgac gttatagcca actccacgaa acgcatccat
     6721 taatttgtca taccttgcat ggtgcaacag atccgttggc gatggtgcac cagcgccgct
     6781 gagatcaaca ccgtctcctc tgtagatgcc gccattatta aaaataacga tcgtcaccgg
     6841 caggttatat cgacaaatcg tttcaatctc catcccactg aaaccaaaag cactatcacc
     6901 ttcaatggcg acaaccggag aaccagaggt cacgctagca ccgatggcat agcccatacc
     6961 gatgcccatg acaccccagg tgccacaatc cagacgacga cgtggtttat acatatcaat
     7021 aatatttcgt gcattatcca gggtatttgc cccttcatta actaaataaa tatcctggtt
     7081 ctcgcgcaaa acatcgcgca cagcactcaa tgcattaaag taatttaatg gttgggtatc
     7141 tgtacttaac ttttcatgca ttttttgtgc attttgctgc ttgtggatat ttaaaatatc
     7201 gcgccacacc agtggagtcg tgaatgtgtt ttgtttcaat tctgccaaca taccttgcat
     7261 actggatgca atatcgccaa cgactggcac agcaatgggg cggttgctgt caatttcctg
     7321 cggttcaata tccagttgaa taaactgtgt ttctgccgcc catccttttt taccgtgtgc
     7381 cagtaaccaa ttcagtcgtg caccaacaag catgacaacg tcagcatttg ccagggcaaa
     7441 cgaacgcgca gctgccgcag agagtggatg cgtatcttcg aggatccctt tcgccataga
     7501 cattggcagg aatggaatct gggcactttc aataaattca cgaagctgtt catcagattg
     7561 tgaatacgcc gcgcctttgc caaggataat taatggtcgt tcagcttttg ctaaaagcga
     7621 aattgcgcta gtgactgact tcgggcatgg caataatgct ggcgacggat tttcaacttt
     7681 aacaatcgtg gttaacgctt cgtctttttc catcgtcgcg gccaggacat ttgctggcaa
     7741 atcaagataa acaccgccag gacgacccga tactgagacc cggatagcgc gtgccaatgc
     7801 aatgccaaga tcctgcggct gattaacgcg aaatgctgct ttggcatacg gttttgccgc
     7861 attcatttgg tccagctctt cataatcacc ttgctggaga tcgacgatcg cgcggtcgct
     7921 ggaaccgcta atcatgatca tcggaaaacc atttaccgtt gcattggcca atgcggtcaa
     7981 accattgagg aatcctggcg cagacactgt caggcagatc cctggttttt gggtaagaaa
     8041 accgcttgct gcagcggcat agcctgccga ctgctcatga cgaaaaccaa tataacgaat
     8101 gccttccgcc tgggcatggc gcgccatatc cgtcacagga atacctacaa caccataaat
     8161 agtgtcaata ttattctgtt ttaatgcttc aacgatgata tgcataccat ctgtcatttg
     8221 aagttgatct gacatgatta ataacctcga aatttttata aattgggagc cagaagcccc
     8281 cgttaatatc agatggcgtg gttttgcttc attgcagcaa tttcatcgtc gctataaccc
     8341 agctcctgca atacagcagc ggtatgttca cctaatagcg gcgcagcttt aatatctggc
     8401 gtaaaggcag agaatttcat tggacaacca actgtcagat attttccacg caacggttgt
     8461 tccacttcga caacactgcc actttggcgc aaagagggat caagtgaaat ttctttcata
     8521 cttaaaaccg gtgcacaagg aatatcaaac tgagtcaaat aggccacagc ttcatgttta
     8581 tcaatagtga cagtgtattt ttcgatttca gcaaaaatat cgaaaatatg tggctgtcgg
     8641 gcatgggctg tactgtatgc cggatcggta atccattctg gtttgccgat ggctttacag
     8701 gtgttttccc agttttgctc ctgaatagtg aaataaatat aggcgttagg atcggtttcc
     8761 cagcctttac atttcaggat ccagccaggc tgaccgcccc cacccgcatt accaccgcgg
     8821 ggaactgcat caccaaatgt gccattcgga tactgcgggt attcttccag ataacccaat
     8881 ttatcgagac gctgctggtc gcgtaatttc acgcggcaaa ggttcaacac ggcatcctgc
     8941 attgacatgg tgactcgttg cccacgcccc gttttttcgc gatgcagcaa agcagcaagt
     9001 aaaccgatca gcaaatgcat tccggtgttg ctgtcaccca acgctgcagc gcttaccagc
     9061 ggcggaccgt cccaaaaacc tgtagtagat gccgcgccac ccgctgcctg agcaacgttt
     9121 tcataggctt ttacattcac ataaggcgaa cactcatcaa accctttgat cgaaccaaaa
     9181 atcagacgtg gattgatttc ttgaatatgc tcccaggtga agcccatatg atcaatggcc
     9241 cctggatgaa agttctcgac taagatatca gcttcgcgga tcagcttttc cattacctct
     9301 ttgccttccg ctgttttggt atttaattca atagaacgtt tgttactgtt aagcatggtg
     9361 aagtaaagcg catcgatatc aggaatatct cgcagctggt ggcgcgttac gtcaccaacg
     9421 ccgggacgtt caattttaat gacgtcagcg ccaaaccagg ccagcatttg agtacaagat
     9481 gggccagatt gcacaccggt gaaatcgaga actttaattc cttgaagtgg agttgacata
     9541 gcaataccta ttagttaatt aaatatgtgt taatttagcg tttatttaag gcataacgcc
     9601 tctataaata atctgcaaaa tatcgtcgta gctgcactcg atatttttaa attcctgtat
     9661 caagtgctga aaatcatttc atcgctactt ccaattcctt tctgcatggt aagtagtgag
     9721 gacgaaaaga agaatataat aaatattggt tcgcggtaat cagagagagc cgcgacattt
     9781 gtgatgttat tcttacaact gtaagaagtg tcagtgattt acaattaagg ttatgattac
     9841 actatttaaa gcgtaccata aaatataaca taataaatga tttttgataa tatgggatta
     9901 ttgcggttta ctttgaggaa gataaaatgc gagtttatag aaccgcgagc accaaataat
     9961 tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcgactgga gggagttaaa
    10021 tcctttacct caatatattg cctgtcaggt ttagttcaca aacagtgctt cgctatcaac
    10081 gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt gctttttatc
    10141 aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca tcaaatattg
    10201 gttttccatt acaccaacac cggccaggcg aagctcttcc attgccccct ttttatcccc
    10261 tttcgccatt ttttcgttgg caatttttat cgcggcttct ttttcgggtg tcgctacata
    10321 actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat tgacgttggt
    10381 ctttttacct ggcttagcga actttgccca ttcggtacta tcatctgaca gcaaagcgga
    10441 agcttcattg gtcagttctt ttgccttttc cggatcgcca tgaaacagag ccaggcgcgc
    10501 tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg ctgctgccgt
    10561 tttctgggtt tgcttaactt gctgagtttg ctgttgtgcc gctaccggtg cattatcagc
    10621 agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa ttaaacgttt
    10681 catacttacc tcattcttta acagttttag aaaacgccgc catcatattg ggtgagcatg
    10741 aaatgagaaa tcagagagca acggacattt tgaaagggtt atcttacaac tgtagttatt
    10801 atcgcaaagc cgcttttagt cttacagttg taggaatctg cttcctgtcg cagagaacta
    10861 ttcttatata
//
LOCUS       DAZGJT010000133        10781 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZGJT010000133 DAZGJT010000000
VERSION     DAZGJT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.133"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..359)
                     /locus_tag="ACHKFK_004167"
     CDS             complement(<1..359)
                     /locus_tag="ACHKFK_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO9054390.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFS"
     gene            complement(425..787)
                     /gene="yfdP"
                     /locus_tag="ACHKFK_004168"
     CDS             complement(425..787)
                     /gene="yfdP"
                     /locus_tag="ACHKFK_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO9054391.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            complement(1454..2128)
                     /gene="ymfK"
                     /locus_tag="ACHKFK_004169"
     CDS             complement(1454..2128)
                     /gene="ymfK"
                     /locus_tag="ACHKFK_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HFO9054392.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            2219..2419
                     /gene="ymfT"
                     /locus_tag="ACHKFK_004170"
     CDS             2219..2419
                     /gene="ymfT"
                     /locus_tag="ACHKFK_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO9054393.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            2463..3020
                     /gene="ymfL"
                     /locus_tag="ACHKFK_004171"
                     /pseudo
     CDS             2463..3020
                     /gene="ymfL"
                     /locus_tag="ACHKFK_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
     gene            3166..3345
                     /locus_tag="ACHKFK_004172"
     CDS             3166..3345
                     /locus_tag="ACHKFK_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9054394.1"
                     /translation="MQELNRWFRDGRGLYVHVIRWEPETQRVIYLRKDYQHECFSPLW
                     KFRRDFVECEGPPAH"
     gene            3275..4177
                     /locus_tag="ACHKFK_004173"
     CDS             3275..4177
                     /locus_tag="ACHKFK_004173"
                     /inference="COORDINATES: protein motif:HMM:TIGR02681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO9054395.1"
                     /translation="MSALVLCGNSGVILLSVKDHQHIDSAIPGRYTVQAPYKAGAGIG
                     VLKLSTAMYDAPASFLSSAFAHIQIMVGWAGAPKGAPVSVDAGYANPVQFTTSEIGVS
                     GGGSFSLSTEAAIMATIPALTQPEITIDNGQAVTTSLAVANSFSKRHDDVLKKIRTLD
                     CSPEFCARNFAETSISVNQPNGGTRKLPCYQITRDGFAFLAMGFTGKRAARFKEAYIN
                     AFNQMEKLLSKPSTLSDAADNASVLYSHLSVIHKVWLQQLYPMLAKAESPLAVSLYDR
                     INDAALLASLINLSLNPSEVRGRK"
     gene            4174..4398
                     /locus_tag="ACHKFK_004174"
     CDS             4174..4398
                     /locus_tag="ACHKFK_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054396.1"
                     /translation="MIRNIFKRFTNQTFRCPRPGQWYTTPAGHVLRVSLVDRECQKVI
                     CEPLGRNYRISMPLIAFCSGKMFKRLGGVA"
     gene            4395..4694
                     /locus_tag="ACHKFK_004175"
     CDS             4395..4694
                     /locus_tag="ACHKFK_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000995578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9054397.1"
                     /translation="MNCFQFVCGCAFDNPIQRLIMLRVLMSGSSDGEGERVIDHQVLA
                     DFCCCSKQAIFRETLALERAGYLHIRKIATLTIDAKARLQPARGYTILMPRKEVV"
     gene            4691..5674
                     /locus_tag="ACHKFK_004176"
     CDS             4691..5674
                     /locus_tag="ACHKFK_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9054398.1"
                     /translation="MSRYAPTPEVMAIGQINISGNVTPANWWKYIRLPSGRPDATAIA
                     LLSEIVYWYRPTEVRDEHTGALLGYRKRFQGDKLQRSYQAFAEQFGFGKRETADALKR
                     LRDAGFITLDLRTVEMLDGVKCSNILFVGINPQAIAAITTPSSVSPESNSNNAISDTA
                     ITLKRNTPRRHNGTGDTLNVDTNTEITTEITTETKNTIDASADASAPARSARQEYSPE
                     FEQAWQEYPKRAGGNSKSAAFKAWKARIREGVTPETMLDGVKRYAAWVRVSGNTGTQF
                     VKQASTFFGPDRHFEESWEVPAVSAARREDPYFKASYDNVDYSQIPAGFRG"
     gene            5671..5754
                     /locus_tag="ACHKFK_004177"
                     /pseudo
     CDS             5671..5754
                     /locus_tag="ACHKFK_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5769..6422
                     /locus_tag="ACHKFK_004178"
     CDS             5769..6422
                     /locus_tag="ACHKFK_004178"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO9054399.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHATAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            6419..6814
                     /locus_tag="ACHKFK_004179"
     CDS             6419..6814
                     /locus_tag="ACHKFK_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9054400.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            6977..7792
                     /locus_tag="ACHKFK_004180"
     CDS             6977..7792
                     /locus_tag="ACHKFK_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO9054401.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETTSDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            7800..8789
                     /locus_tag="ACHKFK_004181"
     CDS             7800..8789
                     /locus_tag="ACHKFK_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO9054402.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRSVFRHEAVIRRAGGVECLESWLLREKGCQWPHSGWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKVLRLPKTVVPSVTRESDLVPSVPATSIIRDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MATKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            8804..9199
                     /locus_tag="ACHKFK_004182"
     CDS             8804..9199
                     /locus_tag="ACHKFK_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9054403.1"
                     /translation="MMRDIQMVLERWGAWAASGNAGVDYSPIAAGFKGLLPSTAKPRP
                     ACSDDDGLIIENCLTRLKKKKPDEYSLLVAHYLLRISKRQIARTRKKSEKAIRIEMQI
                     AEGFIDGCLSMLGVRLEMDDWLPKKVKND"
     gene            9404..9601
                     /locus_tag="ACHKFK_004183"
     CDS             9404..9601
                     /locus_tag="ACHKFK_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9054404.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLPQ"
     gene            complement(9784..9915)
                     /locus_tag="ACHKFK_004184"
                     /pseudo
     CDS             complement(9784..9915)
                     /locus_tag="ACHKFK_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311526.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10657..>10781
                     /locus_tag="ACHKFK_004185"
     CDS             10657..>10781
                     /locus_tag="ACHKFK_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO9054405.1"
                     /translation="MTFKHYDVVRAASPSDLAERLTQKLKEGWQPFGSPVAITPYT"
BASE COUNT         2656 a         2553 c         2837 g         2735 t
ORIGIN      
        1 gagaacggtg ctgtcttttt cagtttgaga gtggcggtgt tatctgcgtg acctggttca
       61 tcaatagtac ccaggttaag cacactgacg gcacgcatat tatcagcatc gataaagcag
      121 cgggtgcctt catctgcaag atctttagaa taacgggtaa agtcatcgat gctggcagtg
      181 gaaagcgcac cacggaaacg gaagcgattt aaattaaatt tttccagatc atgaatgcgg
      241 aaattctcag gcaatgccac agcatcggca ccaatcttac tgataatttc attaacaccc
      301 tgcgcagaaa taagggcatg gatttgatta attgcggttg cgtctaagtt ctgagacata
      361 ataagtcctc actatataaa gatattcagt gatgagataa ataatcagtt aattaagaac
      421 gatattaatg acctgctgcg cggagttttc cgtcaggttc accggcaaga gtcagtaatt
      481 gtccctggtc ttcctgcaga atagtcaggc gaccaccgcg attgacatac atcggcgttt
      541 cggtggtgtc ttcttcggaa attttcccgc ggttagtcgg acgaacatat gagagtttgt
      601 gttttatttt cacacggttc tcatcaaacg gttcgatttc caggttgagc gagaccttcc
      661 ctttggtttt cgtgttcatc acacctgaag cgacttcact gagaacagcg ccgattttgg
      721 tttcaaatac gccgccgtcc agctccccga taaatgcctg cacatcagta ctgcgttcgc
      781 tagccatttt gctgctcctc atcatatcga ccctgcaagg tcggttggtt tctccacaaa
      841 acagagaaga acacctgcgg tggcagccgc ccgggtggat tgggttatga gcccgtcgtc
      901 cggtgatgct cttctctgtt ttgtaaaaag agcggtacca gccggaagca agtgtacaaa
      961 ctggtaccgc caaagcagtg gctgttgtgg tgggcttgtc acttaagcgt atggtcaacc
     1021 tgacaacccg gtgtcctcaa cggggaagga ataaccccgc catacttacc gccgcgccat
     1081 ttcgcggatt accacaacgc tgagagcact tagccagtta cggcaccata ctttgtcgcg
     1141 gttccataaa tgccctcatc gttgcaccct ggtctcttcc caggcgtcaa accgaatcgc
     1201 cacgctggtt aggcgtctta tcagcatcct cattgacttg cacattccgg ctacctggtt
     1261 tgtttgcccg agcaaggagt ggattgtccc ctttaacgtc cccagaccgc taacgacgca
     1321 tgtgccatac gccgtgttac aaccaaattt tgtttaatct tgcctgtgat atgtttcttt
     1381 tagatacatt atgtatctca agggtacatt gtcaagtata aaaaaacctg ccgaagcagg
     1441 ttataaatat tgattaggcc tttattttgt atcttcttgg ttttcctgag aaaattactg
     1501 tgccaattat agagcaatta ccgttgatct taatgtaagg ttcaggccag tttgggttta
     1561 atgctttgag gtaacgctgt gttccatctt ctatcaaccg cttaaaggtg gtttcgcctg
     1621 tatcgtgcat caatgcaata acgtcgtcac cgtggcaggc agggacttca ggatctacaa
     1681 aaatcatgtc tcccgggcgg tactcatcaa tcattgaatc accaatcacc cgcaagatat
     1741 aagtcatttc gccacagggt acagggcagg gataagtttc tgctgtgctc aaatcaacct
     1801 cagaatagcc aacttctttc catgctccgg cctgtaccca tgatatgaca gggactaacg
     1861 ttatttgttt gttagtaatt gaaacatcag gtttttttgt gatgtttgtt gtctggtgtt
     1921 cttgatcaag ccatccgaca ggcaggtcga aacatttttc gatgtgccgt gccatgctgt
     1981 caccgatatt tttagtagca ccatctccca taaacctgct ggtctgggtt ggctcgcgat
     2041 caatcatggt ggcaaaggaa gaattcccgc caacaccatc tctcagtttt ctggcgttag
     2101 accgccggat gtcatggact gttttcataa cgaaattaaa acctttgtac cgatagggta
     2161 caagtatctt gaaggttcat ctcaatcatg taatatgtat atcggaggta catgttgtat
     2221 gaaagcgtat tgggactctt taaccaaaga acagcagggc gagttggccg gaaaagttgg
     2281 ctcaacacca ggctacttac ggctggtttt caatggttat aaaaaagcca gttttgtgct
     2341 ggctaaaaaa cttgagcaat gcacgtcagg tgcaattacg aaatctgact taagaccgga
     2401 tatctatccg aaagattaac agaacacctt caatttttaa ccacagaacg atgaggctaa
     2461 ccgtgggtaa gcatcactgg aaagtagaaa aacagcctga gtggtacgtg aaagctgtca
     2521 gaaaaactat cgcggcgttg ccggggggtt acgctgaagc tgctgagtgg ctggatgtaa
     2581 cagagaacgc tttattcaat cgccttcgtg ccgatggcga tcagattttc ccgctgggat
     2641 gggcaatgat tttacaacgt gctggtggaa ctcacttcat tgctgacgct gtggcgcagt
     2701 ctgcaaatgg cgtctttgtg tctcttcctg acgtcgagga tgtggacaac gccgatatta
     2761 accagcgtct gctggaagtc attgaacaga tcggcagtta ttccagacag attcgttcag
     2821 caatcgaaga cggtgtagtg gaaccgcatg agaagacagc aattaacgac gagctgtacc
     2881 tctcaatttc gaagctctag gagcatgcag cactggtcta caaaatcttc tgcgctccag
     2941 aaaatagtaa cgcccgcgag tgtgcagctc cgggcgtcgt ggcgtcgatt gcttctggtt
     3001 gtggagaaac taacgcatga atagtttaac ggcaaataac cgtttgtcgc aacagctggt
     3061 ggtcagcgtc gctgaacacc tgttgttacg gcatgaatgc agattaccaa atcacctggc
     3121 tgtaagtaac cacagagaac tttacctgac tgtggggggc gagttgtgca ggaacttaac
     3181 cgctggtttc gtgacggaag aggactttat gttcatgtta ttcgttggga gccagaaaca
     3241 cagcgcgtta tctatcttcg caaagactac cagcatgagt gctttagtcc tttgtggaaa
     3301 ttcaggcgtg attttgttga gtgtgaagga ccaccagcac attgattctg ccattccggg
     3361 acgttacact gttcaggcac cttataaagc gggtgccggg attggcgtcc tgaaattgtc
     3421 aacggcgatg tatgacgcgc cagcgtcttt tttatcgtcc gcatttgctc acatccagat
     3481 tatggtgggc tgggcggggg caccgaaagg tgcgccggtc tccgttgacg ccggttacgc
     3541 caaccccgtc cagttcacca ccagtgaaat tggcgtttcc ggtggtggaa gtttttcact
     3601 gtcaacggag gctgccatca tggctacgat cccagccctc actcaacctg aaatcaccat
     3661 tgacaacggc caggccgtta ccacttcttt ggctgttgcc aactccttct ccaagcgtca
     3721 cgacgatgtg ctgaaaaaga tccgcactct ggattgttcc ccagagtttt gtgcccgcaa
     3781 ttttgcggag acatcgattt cggtaaatca accgaacggt ggtacacgca agctcccttg
     3841 ctatcaaatc acacgagacg gttttgcgtt tcttgctatg ggtttcacgg gtaaacgtgc
     3901 tgcccggttc aaagaggcat acatcaatgc ctttaaccag atggaaaaac tgctttcaaa
     3961 gccatccacg ctgagcgatg ccgcagataa cgccagcgtg ctttactccc acctgtcggt
     4021 aatccacaag gtctggctgc agcagcttta tcccatgttg gcaaaagccg aatccccgct
     4081 ggctgtaagt ctgtatgacc gcatcaacga cgcggcgcta ctggccagtc tcataaattt
     4141 gtcgctgaac ccttcagagg taagggggcg caaatgatcc ggaatatttt caaacggttt
     4201 accaatcaga ctttccgttg tcctcgtcct ggtcagtggt acaccacgcc tgcagggcat
     4261 gttctacgtg tcagcctggt tgaccgtgaa tgtcagaagg tgatttgtga accgctgggc
     4321 cgtaattacc gcatcagtat gccgcttata gccttttgct ccggaaaaat gtttaagcgt
     4381 ctgggaggtg tggcgtgaac tgttttcagt ttgtgtgcgg atgtgctttc gataacccga
     4441 ttcagcgcct gattatgttg cgtgttttga tgtcgggttc ttcagacggt gaaggcgaga
     4501 gagttattga tcatcaggtg cttgctgatt tctgctgttg ttctaagcaa gcgatattca
     4561 gggaaaccct ggcactggaa agagctggtt atcttcatat ccgaaaaatt gcaacgctta
     4621 ctattgatgc aaaagccaga ctacaacctg cgcgtggcta cacaattctc atgccgcgga
     4681 aggaggttgt atgagccgtt acgcccccac accggaagtt atggctattg gtcaaattaa
     4741 tatttccggc aatgttacac ctgcgaactg gtggaaatat attcgactac ccagtgggcg
     4801 tccggatgcg acggctatcg ctctgctttc agagatcgtt tactggtacc gcccgacaga
     4861 ggtcagggat gagcacaccg gagcgttgct gggatatcgc aagcgttttc agggcgacaa
     4921 actgcaaaga agctaccagg cgtttgctga gcagtttggt ttcgggaaaa gggaaaccgc
     4981 agatgcgctg aagcgtctgc gcgatgcagg gtttattact ctggatttac gcacggtgga
     5041 aatgctcgat ggggtgaaat gcagcaatat tttgtttgtc gggatcaacc cacaggcaat
     5101 tgcggccatc accacacctt cttctgtttc gccagaaagt aacagcaata atgcaatcag
     5161 cgatacagct attacgttaa aacggaacac cccccgacgt cataacggaa caggggatac
     5221 gctgaatgtt gatacaaata cagagattac tacagagatt acaacggaga ctaaaaacac
     5281 tattgatgca tccgctgacg cgtctgcgcc agcgcgttct gcccgacagg aatattcacc
     5341 ggaatttgaa caggcctggc aggaatatcc caaacgtgct ggtggtaatt caaaatccgc
     5401 cgcttttaaa gcctggaaag cccgaatcag ggaaggtgtg acacccgaaa ccatgctcga
     5461 cggtgtgaaa cgctatgccg cctgggtgcg tgtctctgga aataccggta cccagttcgt
     5521 gaagcaggcg tcgacgttct ttggtccgga tcgtcatttc gaagaatcct gggaagttcc
     5581 tgcggtatct gcagccagac gcgaggaccc gtacttcaaa gccagttacg acaacgtgga
     5641 ctacagccag attccggcag gattcagggg gtgatcatga gtcttttgaa tgaagttcag
     5701 aaattcattg aagcccatcc tggctgtact tccggagaca ttgcggatgc tttttacgtg
     5761 ggggcttaat gagtaataaa tattgccagg cgctggtgga actgcggaat aaaccagccc
     5821 atgaactgaa ggaagtggga gatcagtggc gcacgccgga taacattttc tggggaatta
     5881 acaccttgtt tggtccgttt gttctggatc tgttcactga cggtgataac gccaaatgtg
     5941 ccgcgtatta cacggcggaa gacaacgcgc tggcgcatga ctggtcagaa cgtcttgcgg
     6001 agcttaaagg tgctgccttt ggcaatcccc cgtacagccg cgccagtcag catgaggggc
     6061 aatacatcac cggcatgcgt tacatcatga agcatgccac tgccatgcgt gataaaggcg
     6121 ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt gtggtggccg gaagatgcag
     6181 atcatattgc ttttattcgc gggcgtattg gttttgaact gcctgcctgg tttatcccga
     6241 aagacgaaaa gcaggtgcca acaggtgctt tcttcgctgg tgctattgct gttttcgaca
     6301 agacctggaa gggaccggca atcagctaca tcgggcgcga tgaacttgag gcatgtggtg
     6361 aggcatttct ggcgcaggtt cgccagcagg cggaaaaact ggtcagggag atggtggcat
     6421 gaagctaata ctgccttttc cgcccagcgt gaacacgtac tggcgacacc ccaacaaagg
     6481 ggcgtttgct ggtaagagcc tgataagcgc ggcggggcga aaattccaga gcgcggcgtg
     6541 tgcagcaata gttgagcagt tacgtcgtct gccgaaacca acgtcggcac ctgcttcagt
     6601 ggagatcgtg ttgtttcctc cggataaccg gatccgcgat ctggacaact ataacaaggc
     6661 gctgtttgac gccctgaccc acgcgggtgt gtgggaagac gacagccagg tgaaaagaat
     6721 gctggtggag tggggaccgg ttatcccgga agggaaggtc gagatcacta tcagtaagta
     6781 cgaaaaagcg agttgcaaat tagcaactcg gtaacggaat tgagcaacac cctaaatttg
     6841 ggtattacct cgttaaagat actgtattta tgaacagtgt atccttgata actattaaaa
     6901 atcgcagtaa gttcatcctg catcaacgaa aagggagtgc agtcccgctc gtggataaaa
     6961 atttgtggag aaaccaatga atcagttgct tgtaattgat ggcgtttctg tgcgccagta
     7021 cttcgaatct aactactgtc ttaacgacct tcagaaagct gctcttcttg ccgctggtga
     7081 gaatcgctcc tcccgttcgc tggaagttca cgagtttatg cgtcgtcctg aaacgaaggc
     7141 tcttgtggaa ttattggaag aagaaactac gggagattcc cgtagtattc ctgtcatcac
     7201 cattcagggg cgcaatggtg ggacgtatgt ctgtaaagag ctggtctatg catatgcaat
     7261 gtggatcagc ccggcattca gcttaaaagt gatacgtact tttgatgcgc ttcataattc
     7321 atcaccagaa gaaaccacat ccgacaaaat taaatccggg gtcattctgc ttgaatcagc
     7381 agcaaagact ctaaatctgt caaactcctc gaaacttggt gcataccaga aattatcaaa
     7441 ggtagctggt cttcctgaac ttatgccgat ctatgccatt gatgcacctg ctgatgcgcc
     7501 agatggttca agccgcccta cgctgtcgct gagtgcactg ctgaagcagt atggtatccg
     7561 cctgacggct aatcaggcat atcaccagat ggcgaagctg gggatcgttg aacaacgcga
     7621 acgatacagc cgtaccgcga ttaacaacat caaaaaattc tggtcgctga ccgcgaaagg
     7681 ctgcatgttc ggcaagaaca tcaccagtcc tgcaaatccg cgcgagacgc agccgcattt
     7741 cttcgaatcc cgatttcctg agctgttaaa gctgctcgat accgttcatt gaggtgactg
     7801 tgagagcact actgacccct gaaattgccc cgcgtatggg gatcgtattg ttcaggccag
     7861 gttcagagct gatgcccctg tttatgcagg ggcgtgtcct gctggagcct gagccggagc
     7921 gttattcatc tttcgccagt ggtgccgttc cggcggcatc acaaccgctg gcggatgatc
     7981 ctgccgttcg gtccgtgttc cgccatgagg cagtgatccg tcgtgctggt ggcgtggaat
     8041 gtcttgaaag ctggttactt cgtgaaaaag gctgccagtg gcctcattcc ggatggcaca
     8101 gcgagaacat gaccacaatg cgacacgctc cgggcgcaat ccgtctgtgc tggcactgcg
     8161 ataaccagct gcgcgatcag ttcacggaac ggctggaatc aatggcaacg gataactgtg
     8221 cccgctgggt gttgtctgtt gtgcgtcggg atctcggttt tgatgacagt cacgttgtga
     8281 caatgccgga actgtgctgg tggctgattc gtaatgacct ggcggatgcc ttaccggaaa
     8341 gtgcagcccg taaggtactg agattaccga agacggttgt gccgtctgtc acccgggaaa
     8401 gtgaccttgt gccttcggtt cccgccacca gtattatccg ggataaagcg aaaaaggtgc
     8461 tggcgctgaa agtggatccg gagtcgccgg agtcttttat gttacgccca aaacgtcgcc
     8521 gctgggttaa tgaaaagtac acgcgctggg ttaagacgca gccgtgcgca tgttgtggaa
     8581 agcctgctga tgatccccac cacctgatag gtcacggtca gggtggaatg gctacaaaag
     8641 cgcatgacct ctttgtgttg cctttgtgca gaaagcatca cgacgagctg catgcggata
     8701 ccgtggcatt tgaagagaag tatggctccc agctggagct gatatttcgt tttatcgatc
     8761 gcgcgctggc gattggtgtg ctgtcctgat tttgtggaga aagttgatgc gtgatattca
     8821 gatggttctc gaacgctggg gggcatgggc ggcaagtggt aacgccgggg tggactattc
     8881 tccgatagct gctggattta aaggcctttt accatccacc gctaaacctc gcccggcctg
     8941 cagcgatgat gacggcctta tcatcgaaaa ctgccttacg cgcctgaaga agaaaaaacc
     9001 ggacgagtat tcgctgctgg tagctcatta tctgctgcgc atatcaaaaa ggcagattgc
     9061 cagaacaaga aagaagagcg aaaaggcaat acgaattgag atgcagattg ctgaaggatt
     9121 tattgacgga tgtctgtcga tgctgggtgt aaggctggag atggacgact ggctgcccaa
     9181 aaaagtaaaa aatgattagc gcggtccgca aaaagtatgt cagtatgtta agagtggtta
     9241 ctacgccaca caacttaaac ccgccgcctg gcgggttttt tatgactgaa atcgcatcag
     9301 tacagtaaac gtgctggtgg tggtgaatac cggtctttca gcttgctggc tttttcgaca
     9361 agagttattg gtgtgtcacg ttaaccggaa aagggaaaaa gacatgctaa aacagcagga
     9421 tatgacagaa accgccagag tggtgtttaa tgaattaagt gccaccgaac cggcgacagt
     9481 cggggagatt gcgcagaata cttacctttc acgcgaacgc tgccagttaa tactgaccca
     9541 gctggttatg gcgggtctgg cagactatca gttcggttgt tacagacgcc ttccgcagtg
     9601 aaggattttt tatttgtggt aaatgggcgg ctggtgggtg tggtggttgt tgctttcccg
     9661 ttgctgaaaa agaaagcatc aggcgattag cagggtatca gttacccgtt gaaattttta
     9721 aatacctcac aattcaggcg gttgactgtt gtctggtttg cggggagttt gttaaaagaa
     9781 actggcatgg tgaatccccc tgtgcggagg ggcaatcagc aactggtgtt ttgtcaccga
     9841 cccttatcct ttctgtgcgg gttcaggtgc tgatactgaa ctcaccggga ggcacccggc
     9901 accatgcaat ggcacatagc gccactctcc agcccctctc cggaggggct tttctgtgcc
     9961 ggatacatca cagtttctgg aaccttgggt actacagtat cagtcagggt gctatatttt
    10021 cagatgtgat gaaagcctgt cagcaggcag ggcgtatcgg aaatgaccca gtagagaaaa
    10081 cgttgactca gataccggtg ctgagttacc gggaaaccgg catcacatga ccgctatcct
    10141 tccaggccca tccgctccgg tgggcctttt tactgcagaa aacaggttcc ccgttaaatg
    10201 ctatgttgct cacaattcag taagttgaca gttgcctgtc agactgggca tttgttaaaa
    10261 aaatttcgca tggtgaatcc ccctgagcgg aggggcgact ggtgacggta taatttctga
    10321 ttatcaaaac gagaatgacg cgggtttagt ggcaccgggc tgaactcacc gggaggcacc
    10381 cggcaccatg tgcatgatga tacagatacg cggctttagc ccctctccgg aggggctttc
    10441 ttgtgggcaa aaaaaagcct gagtgggttc gggcaacagc atgagatata catttttata
    10501 atcgaatgga ttttaaccag aattcataag gctgcgcaac tgcgtggcct ttttcgtatt
    10561 gcgggctgta gttttcctcc tgccattgtc ctgtaacttc cggacttcag cccgcccctc
    10621 atctgactca caacattatc ccgaccggga ggattcatga catttaaaca ctacgatgtg
    10681 gtcagggcgg cgtcgccgtc agaccttgcg gagcgactga cacaaaaact gaaggagggg
    10741 tggcagccat ttggcagccc tgtcgccatc acgccttata c
//
LOCUS       DAZGJT010000134        10562 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZGJT010000134 DAZGJT010000000
VERSION     DAZGJT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.135"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..1000
                     /locus_tag="ACHKFK_004186"
     CDS             92..1000
                     /locus_tag="ACHKFK_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HFO9054406.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENVFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIEDRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRENPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1219..3210)
                     /gene="tkt"
                     /locus_tag="ACHKFK_004187"
     CDS             complement(1219..3210)
                     /gene="tkt"
                     /locus_tag="ACHKFK_004187"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO9054407.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQIASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAEQLFEEFGFTVDNVVAKAKALL"
     gene            3488..4246
                     /gene="loiP"
                     /locus_tag="ACHKFK_004188"
                     /gene_synonym="yggG"
     CDS             3488..4246
                     /gene="loiP"
                     /locus_tag="ACHKFK_004188"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO9054408.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSSGLLSSGAEAFQAYSLSDAQ
                     VKKLSDQACQEMDSKATIAPANSEYARRLTTISRALGDNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVLGHEMGHVALGHVKKGMQVALGTNAIRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFTQRQESEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(4450..5370)
                     /gene="speB"
                     /locus_tag="ACHKFK_004189"
     CDS             complement(4450..5370)
                     /gene="speB"
                     /locus_tag="ACHKFK_004189"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO9054409.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLTAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDIIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5506..6237)
                     /locus_tag="ACHKFK_004190"
     CDS             complement(5506..6237)
                     /locus_tag="ACHKFK_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054410.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIIISYSTNVPSGYDRVYSIRINELEYAIRDGNFKSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFVAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(6382..8358)
                     /gene="speA"
                     /locus_tag="ACHKFK_004191"
     CDS             complement(6382..8358)
                     /gene="speA"
                     /locus_tag="ACHKFK_004191"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO9054411.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(8367..8498)
                     /gene="yqgB"
                     /locus_tag="ACHKFK_004192"
     CDS             complement(8367..8498)
                     /gene="yqgB"
                     /locus_tag="ACHKFK_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO9054412.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(8592..8735)
                     /gene="yqgG"
                     /locus_tag="ACHKFK_004193"
     CDS             complement(8592..8735)
                     /gene="yqgG"
                     /locus_tag="ACHKFK_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO9054413.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(8846..9097)
                     /gene="yqgD"
                     /locus_tag="ACHKFK_004194"
     CDS             complement(8846..9097)
                     /gene="yqgD"
                     /locus_tag="ACHKFK_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO9054414.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTQFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            9153..10307
                     /gene="metK"
                     /locus_tag="ACHKFK_004195"
     CDS             9153..10307
                     /gene="metK"
                     /locus_tag="ACHKFK_004195"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO9054415.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         2626 a         2827 c         2667 g         2442 t
ORIGIN      
        1 tcccctttat aaattctgaa ttatcggctg ttgtctgttt tctcatcacc ccatgtgaca
       61 tattcctata ctttactcaa ggaagattca tatgaaagca cctgtttcat taacctcatt
      121 tcgcccacaa aaatctcttg ctatcgccat tggcgtgctt gccgtggttg tgctgccatt
      181 tctctcgtat tacaccgtta atgaaggtga acgcggcatt ttgctgcgct acggaaaaat
      241 cgtaaaagtt gccgagccgg ggctgggttt taagatccct tttatggaaa gcgtagagaa
      301 aatttccacg cgtaatcagg ctgtggtcta tcagggatta caggcctaca gccgcgacca
      361 gcaaccggca caaatgacag tatcagtcag cttccacatt aaaccttcag aagcaggcgc
      421 ggtttatacc acctacaaca ccattgaagc attaaaagat cgtctgattg tccgtcagtt
      481 gccaactcaa ctggagaacg ttttcggaca atataccgcc atcagcgccg tacaggatcg
      541 tacaaaactg gttcaggatc tgcaaaatgc catgcgcaaa gccgttgttg ggccagtggt
      601 aattgacggg gtccagattg aaaatattga tttttctgac gcttatgaaa agtcgattga
      661 agaccgaatg aaagcagaag tcgccattgc cacgcgtaag caaaatctgg aaacggagaa
      721 aatccaggcg caaatagcgg taactcaggc ccaagcggaa gcagacagta aactagcggc
      781 agccaaagca gaagcggaaa ctattcgcgt aagaggcgcg gcagaggctg aaactattcg
      841 tttaaaaagt gcggctgaag cagaagctat ccgcctgcgc ggtgaagctc tgcgggagaa
      901 tcccggactt gttgccctca ccactgctga gcgttgggac ggtaaactgc ccgataccat
      961 gatccccggc agcacagttc cgtttatctc tactaagtaa aaaagggccg ctttcgcggc
     1021 ccttgagact gatagcaaag acaaaactgc ctgatgcgct acgcttatca ggcctacata
     1081 cttcctgcaa tatgttgaat ttgcaaaatc ttgtaggacg gataaggcgt tcacgccgca
     1141 tccggcataa acaacgcgca ctttgccaac aatctgaagg gccgcatttg cggcccttct
     1201 cacaaagcat cttaccgatt acagcagcgc ttttgctttc gcaacaacgt tgtcaacggt
     1261 gaagccgaac tcttcaaaca gctgctctgc cggagcagat tcaccgaagg tggtcatgcc
     1321 gacgatagca ccgttcaggc caacatactt gtaccagtag tcagcaatgc cagcttccac
     1381 cgcaacacga gcggtaaccg ctttcggcag tacggattca cggtaagccg cgtcctgctt
     1441 gtcgaatgcg tcggtagacg gcatggaaac cacgcgcgct ttcacgcctt cggcagtcag
     1501 tttttcgtag gcggcaacag ccagttcaac ttcagaaccg gtcgcgatga agatcagttc
     1561 cggctgacca gcacagtctt tcagcacata accaccgcgc gcgatgtttg ccagctgctc
     1621 ttcagtacgt tcctgctgcg ccaggttctg acgggagagg ataagcgcag tcgggccgtc
     1681 ctgacgctca acgccgtatt tccacgctac tgcggattca acctggtcac acggacgcca
     1741 tgtagacatg ttcggggtca cgcgcagaga agcgatctgt tctaccggct ggtgagtcgg
     1801 gccatcttcg cccagaccga tggagtcgtg ggtgtaaacc atcacctgac gctgtttcat
     1861 cagcgcagcc atacgtacgg cattacgtgc gtattccacg aacatcagga aggttgaggt
     1921 atacggcagg aaaccaccgt gcagggagat accgttggca atcgcagtca taccgaattc
     1981 acgtacaccg tagtggatgt agttacccgc agcatcttcg ttgattgctt tagaaccaga
     2041 ccacagggtc aggttagacg gcgccaggtc tgcagaaccg ccgaggaatt ccggcaacag
     2101 cggcccgaac gcttcgatag cattctgaga cgctttacgg ctggcgatct tcgccggatt
     2161 agcctgaagt ttagcgatga actctttcgc tttggcgtcg aagtcagacg gcatttcgcc
     2221 tttcatacgg cgggtaaatt cagcggcttc ctgcggataa gctttcgcgt aagcagcgaa
     2281 tttctcgttc catgcggatt ctttcgcctg gcctgcttct ttcgcatccc actgagcata
     2341 gatttcagac gggatttcga acggcgcgta tttccagccc agctgttcgc gggtcagggc
     2401 aatttcagcg tcgcccagcg gcgcaccgtg ggagtcgtgg gtaccggctt tgttcgggga
     2461 accgaaaccg atgatggttt tgcacatcag cagggacggt ttgtcagtca ctgcgcgcgc
     2521 ttcttctact gcgcgtttga tggatgccgc gtcatgaccg tcgatatcgc gaataacgtg
     2581 ccagccgtaa gcttcgaaac gcattgcggt gtcgtcggta aaccagcctt caacgtgacc
     2641 atcgatagaa ataccgttgt catcgtagaa cgcaatcagt ttacccagct tcagcgtacc
     2701 cgccagagag caaacttcgt gggagatgcc ttccatcatg cagccgtcgc ccatgaaggc
     2761 gtaggtgtag tggtcgacga tgtcgtggcc cggacggtta aactgcgccg ccagcgtttt
     2821 ttctgcaatc gccataccga ctgcgttggc aataccctga cccagcggac cggtggtggt
     2881 ttctacacct gcggtgtaac ccacttccgg gtgacccgga gttttagagt gcagctgacg
     2941 gaagtttttc agttcttcca tcggcagatc gtaaccggtg aggtgcagca ggctgtagat
     3001 cagcatggag ccgtggccgt tggacagcac gaagcggtca cggtcagccc aggacggatt
     3061 ctgcgggttg tgtttcagga aatcacgcca caggacttcg gcaatgtcag ccatacccat
     3121 aggggcaccc gggtgaccgg atttggcttt ctgtactgcg tccatgctca gcgcacgaat
     3181 agcattggca agctctttac gtgaggacat tttgactcca gatcggatga tgaagggcac
     3241 gcccttaacg acttgacgac agcgcgtttt gggctacgcc ggaaaatttg ccaacaattt
     3301 accgcaagcc gctcgtcatg tacatggaac atccttttgc cgcttcagaa atctctggat
     3361 catgctcgca tgttgcgcaa tccactcgcc cgtccgctgc gcttttcctt atactgagac
     3421 tgagcgtcga ttcacctgca aacggcgcat ttttagaata atcctgacct tgtgcggaag
     3481 agaaaacatg aaaattcgcg ccttattggt agcaatgagc gtggctacgg tgctgaccgg
     3541 ttgccagaat atggactcca gtggactgct ctcatcagga gcggaagctt ttcaggctta
     3601 cagtttgagt gatgcgcagg tgaaaaaact gagcgatcag gcatgccagg agatggacag
     3661 caaagcgacg attgcgccag ccaatagcga atatgctaga cgtctgacga caatttctcg
     3721 cgcgttaggc gataacatca acggtcagcc ggtgaattac aaagtgtata tggcgaagga
     3781 tgtgaacgcc tttgcaatgg ctaacggctg tatccgcgtc tatagcgggc tgatggatat
     3841 gatgacggat aacgaagtcg aagcggtgct cggtcacgaa atggggcacg tggcattagg
     3901 ccatgtgaag aaaggaatgc aggtagcact tggtacaaac gcaattcgcg ttgcggcggc
     3961 ctctgcaggc ggaattgtcg gcagtttgtc acaatcacaa ctgggagacc tgggcgaaaa
     4021 gctagtcaat tcgcaattta cccaacgtca ggaatcagag gccgatgatt actcctacga
     4081 tttgctgcgc caacgcggta tcagcccggc aggtcttgcc accagctttg aaaaactggc
     4141 gaaactagaa gaaggccgcc aaagctcaat gtttgacgac catcctgcat ccgccgaacg
     4201 cgcccagcat attcgcgatc gtatgagcgc ggatggagtt aagtaaagcc aggtagtgcc
     4261 gaacgtaggt cggataaggc gttcacgccg catctgacaa gaatggcaca tttgttactc
     4321 cgtgcgcatt gccggatgcg atgctggcgt atcttatccg gcctacgggt gccgagtgta
     4381 ggtcggataa ggcgttcacg ccgcatccga cattaatggc acatcacccg cgcgcctctt
     4441 atctggcgct tactcgccct ttttcgccgc ctggatatac agcatctcca gcgccagcgt
     4501 cgccgcagcc agagcggtga tttccgactg atcgtatgcc ggagccacct ccactacgtc
     4561 catcccaacg atgttgagat ctttcaagcc gcgtaccagt ttaatggcgc gatcggaggt
     4621 caggccgcca attaccggcg taccggtgcc tggtgcaaaa gcaggatcca ggcagtcgat
     4681 atcaaaggtc aggtaaaccg gcatatcacc cacaatctgt ttcacctggg cgataatgtc
     4741 atccacgctg cgatcgttca cctggcaggc gtccagcacg gtaaagccgt tgtctttatc
     4801 aaactcggta cgaataccaa tctgcacaga atgattcggg tcgatcagac cttctttcgg
     4861 cgcggtatag aacatggtgc cgtggtcaaa ttcacaaccg ttagcgtagg tatcggtgtg
     4921 ggcatcaaag tgtaccaacg ccattttgcc gaaatgcttc gcatgagcgc gcagcagcgg
     4981 cagcgtaaca aagtggtcac caccgaagga gagcatacgc ttaccggcag tcagcagctt
     5041 ctcggcgtgc gcctgcagct tctcgctcat ctcacgggca tctccgaagg catataccag
     5101 atcgccgcag tccacgacgt tcaggcgctc acgcatgtcg aaattccacg ggaagcgatt
     5161 gtgttcccag gccagattcg tcgaaacctg acggatcgct gccggaccgt ggcgaccacc
     5221 cgcacgacca gaagtggcca tatcgaacgg cacgccagta atcacccagt cagcatcgct
     5281 gtcatacggc tggaagttca tcggcaggcg taaaaaacca aaggcgttgg aaaccagtga
     5341 gttatcgtat tgatgaccta aggtgctcat ggatggactc ctattacaaa gtcgatataa
     5401 aaaaacccct ccgcgtcgtt aggcccgacg aggaagggtt ggatttgcga aacacaagtt
     5461 tgtgctaatt atcgccctaa atgggcaagg catcaagcaa ggttactatt tccgacacgg
     5521 tggcggcgca tagccattgt tatctgccgg aaacgcttgt ccagagcacg gcggcagcgc
     5581 gttttgcgcc gggccttgta attcaaaggc gacaaagccg attacaccgg tattacgcac
     5641 atcgccttgc gccgaattgg ctgcgtagga atcagcaacg ttggcgaact gaaaagccgc
     5701 ttcggtatgt ttatctttgc ggaatccttt aatcgctaac gaatctccgg cattcacaat
     5761 ataaccgttg ttattcagcg aaccttgttt accattcaga acatccaggc catcaaccgt
     5821 agcaacgatc tcgtagttgg tgttacgact gtaattacgg acatataatt gataattcat
     5881 tccgactctg gcatggacaa tatagcgtgg ttcattattg gatgaatcat aaacccgcgt
     5941 aattggtaga gatttaaaat taccatcgcg aatagcatat tccagttcgt taatacgaat
     6001 gctataaacg cggtcgtagc cggaaggaac attagtcgaa taactaataa tgatcatctc
     6061 tgccggatcg cgccaagctc gttcagcaga tacgccctga accgttgagt gcacatcccc
     6121 accccaggcg gtgccgacat tacccgatgc actgctatta tactgcgcat tactggaaca
     6181 accggccagc agcacgatta atgccaccaa cgcgctacgt actttccatt ttttcataac
     6241 cgttcccctg catttaatta atgcagcgat tgtagggaga ttatttaata agaaaagcag
     6301 ctaaattatt tttaagaata tataaaaaga aagcgggccg atttacgtaa ataaatcgcc
     6361 ccgccagcgg aataatctta attattcatc ttcaagatag gtataaccgt acaaacctgc
     6421 ctcgaactct tcaaggaact gttgttgcag ttcagcatca agatcggttt tcttcacctg
     6481 atcgcggaac tgggttaaca gcgttttcgg atcgagctgc acatattgca gcatgtccgc
     6541 cacggtatcg ccttcgtcag acagttccac ttctacgctg ccgtcaggga agacgaacac
     6601 gtcaaccgct tcggtatcac cgaacaggtt gtgcatgttg ccgaggatct cctgatatgc
     6661 gccgaccata aagaaaccga gcatcggcgg attctccgga tcgtactccg gcataggcat
     6721 tgtcgtggca ataccgtcac catcaatata gtggtcgata gcaccgtctg agtcacaggt
     6781 aatatccagc agcacagcgc ggcgttccgg cacttgatcc agcccttcca gcggcagaac
     6841 cgggaacaac tggtcgatcc cccacgcatc cggcatcgac tggaacagcg agaagttgac
     6901 gtacattttg tccgccatac gttcctgcag ctcgtcgata atcggacgat gagcacggtt
     6961 ttgcggatcc agctgctttt gcacttcatg gcacatgctc aaataaagct gctcagccca
     7021 tgcgcgttct tgcaggctaa aggtgccgga agagtagccg atatgaatgt cgtgcagatc
     7081 catctggctg tcgtgtaacc attcacgcag agaacggcga gttcccggtt cgtgcatttc
     7141 ctgccaggtt tcccacatgc tttgcagggc gcgcggtgcg tcttctgcag gcgcggtcgg
     7201 caccgtgtat tcgttacgtt ccacgccgat gatattcgac accaacacgg tgtgatgcgc
     7261 agtcaccgca cgacccgatt cggtgattac cgtcggatgt ggcagaccat tttcttcaca
     7321 cgcatcacca atcgcccaga tgatgttgtt ggcgtattca ttgaggccgt agtttacgga
     7381 gcagtcagac tgcgaacggg tgccttcata gtccacgccc agaccgccgc cgacgtcgaa
     7441 gcactgaata ttgacgccca gcttgtgcag ttccacatag aaacgcgcgg attcacgaac
     7501 gcctgtcgcg atatcgcgaa tattcgccat ctgcgaaccg aggtggaagt gcagcagttg
     7561 caggctgtcg agacgcccgg cttcacgcag cgtttcaacc agttgcagta cttgagtcgc
     7621 cgccaggcca aacttcgatt tttccccgcc ggaggactgc catttacccg agccttgcga
     7681 cgccagacgt gcacgcacgc ccagacgagg aacgacattc agacgttctg cttcatccag
     7741 cacaatggca atttctgaca tcttctcaat gaccagatag accttgtggc ccatcttctc
     7801 gccaattaat gccaggcgga tatattcgcg gtccttataa ccgttgcaga cgatgacgct
     7861 acgggtcatg ccagcatgtg ccagcaccgc catcaactca gctttggaac cggcttccag
     7921 gcccagcggt tcgcccgaat gaatcaggga ctcaatcacg cggcggtgct ggttaacttt
     7981 gatcggataa acgaggaagt aatcgccgtt atagccgtag gattcccgcg ctcgtttgaa
     8041 cgcggcgtta atggaacgca aacggtgctg caggatctgt gggaaacaga acagtgcagg
     8101 cagacgctga ccctgtgctt cgcgagtttt cactaactgc gcgagatcga cgcgagcttc
     8161 cgggacgtcc gggtccgggc acacgctgat gtggcccagc tcgttaacgt catagtagtt
     8221 attgccccac caggcaatat tgtaagtacg cagcatcttg ctggcttcct gggagctcat
     8281 tgcaacctcc tgcatggagc gtagtacacc gtgttcgccc gctgacgaag gcaaacccat
     8341 agacatgtcg tcagacatag cgaacctcaa attattttat taagtgtaaa acagttaacg
     8401 actatcgcag cctggaactg cgataacaac ccataagcac atggattttc cagcagtgaa
     8461 tgctgacgct ccaactgcgc aaccggtttc tttttcataa cattattaag cacataaccg
     8521 aacgtaagtg tgacagttcg gcgaaaccac gagaaaactc ttgtttttac aagagcgccc
     8581 ttgttcagtc ctcagtagct gtaaccagct cttgaatcct gagaagcgcc gagatgggta
     8641 taacatcggc aggtatgcaa agcagagatg cagagtgcgg ggaacgaatc ttcaccagaa
     8701 cggtgagaca ggttaagcag cagacaacgg ttcattattt cgtatcacct ccacggccgc
     8761 ctgttaagac gaacccacaa gccaaaactc tgttttcaac ccggctggaa gtggcaacac
     8821 gaaagaaacg tcgtgtgctt tttatttaag ccgcgcgccg cgttttatac cccacaatgg
     8881 cagaaaattg caaaagataa atacgcagaa tgccagcatt gtcaggaaaa atttccagcc
     8941 acgtttttaa cagaatgaga cacgattcaa aaaaaagtgg aaattgggtg aagaactgac
     9001 ctaaaatagc catccagatg ttaatccatc cataccgatt aacactcaga ctgccagtgt
     9061 ttttaacctg cagagtcgtg gtaggatccg ctaccacaga aaatccacac aacagtttga
     9121 gctaaccaaa ttctctttag gtgatattaa atatggcaaa acaccttttt acgtccgagt
     9181 ccgtctctga agggcatcct gacaaaattg ctgaccaaat ctctgatgcc gttttagacg
     9241 cgatcctcga acaggatccg aaagcacgcg ttgcttgcga aacctacgta aaaaccggca
     9301 tggttttagt tggcggcgaa atcaccacca gcgcctgggt agacatcgaa gagatcaccc
     9361 gtaacaccgt tcgcgaaatt ggctatgtac attccgacat gggctttgac gctaactcct
     9421 gtgcggttct gagcgccatc ggtaaacagt ctcctgacat caaccagggc gttgaccgtg
     9481 ccgatccgct ggaacagggc gcgggcgacc agggcctgat gtttggctac gcaactaatg
     9541 aaaccgacgt gctgatgcca gcacctatca cttatgcaca tcgtctggta cagcgtcagg
     9601 ctgaagtgcg taaaaacggc actctgccgt ggctgcgccc ggacgcgaaa agccaggtga
     9661 ctttccagta tgacgacggc aaaatcgttg gcattgatgc tgtcgtgctt tccactcagc
     9721 actctgaaga gatcgaccag aaatcgctgc aagaagcggt aatggaagag atcatcaagc
     9781 cgattctgcc cgctgaatgg ctgacttctg ccaccaaatt cttcatcaac ccgaccggtc
     9841 gtttcgttat cggtggcccg atgggtgact gcggtctgac tggtcgtaaa atcatcgttg
     9901 atacctacgg cggcatggcg cgtcatggtg gcggtgcatt ctccggtaaa gatccatcaa
     9961 aagtggaccg ttccgcagcc tacgcagcac gttatgtcgc gaaaaacatt gttgctgctg
    10021 gcctggccga tcgttgtgaa attcaggttt cctacgcaat cggcgtggct gaaccgactt
    10081 ccatcatggt agaaactttc ggtactgaga aagtgccttc tgaacaactg actctgctgg
    10141 tacgtgaatt cttcgacctg cgcccatacg gtctgattca gatgctggat ctgctgcacc
    10201 cgatctacaa agaaaccgca gcatacggtc actttggtcg tgaacatttc ccgtgggaaa
    10261 aaaccgacaa agcgcagctg ctgcgtgatg ctgccggtct gaagtaatct ttcttcacct
    10321 gctttcaaag gccagcctcg cgctggcctt tttcttttac ctcaccgcgg tggacaggca
    10381 ttcacgccgc atccggcaaa aaaaccgacc gcacaataac atcattcttc ctcaacacgt
    10441 tttaccgcaa attatcatca ctactgaaac cgattacacc aaccacgcca gccaaatatt
    10501 tgtaatattt tcatattatt attcagcctt cacagttgtt acatttcttt tcagtaaagt
    10561 ct
//
LOCUS       DAZGJT010000135        10409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZGJT010000135 DAZGJT010000000
VERSION     DAZGJT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.136"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..437)
                     /locus_tag="ACHKFK_004196"
     CDS             complement(<1..437)
                     /locus_tag="ACHKFK_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054416.1"
                     /translation="MTTYNTGNPLGSSAAKDLYDNAQNFDHLSNDQSNELWPDRFGNP
                     RLTWHGMESKYKESLSKMGWILIKSFQVGAEITLTNQALHDETSGEYYRWDGVLPKTV
                     PAGSTPETTGGIGLGAWVSVGDASLRGDLLSLDGEKYIGICPD"
     gene            complement(490..3207)
                     /locus_tag="ACHKFK_004197"
     CDS             complement(490..3207)
                     /locus_tag="ACHKFK_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity factor TipJ family phage tail
                     protein"
                     /protein_id="HFO9054417.1"
                     /translation="MTIRIYPSRLPGEPLETHEHGAVTIHQWLAKNVQGYKPDMKHPI
                     TVDVDGENIPPQAWFEFAIKSDSDVRIYPVPYGAVALAWIAVAVSVASVAYALFFAPG
                     VGDLGGYSSGTGNPLDVNPAKANNAKLGDPIRELFGRSRIYPDYAVQPVTRFSVDDPT
                     VMTVEMFVVMGRGRFSFGDGDIRVGSTPIASLGDGFNYTVYQPGQSVSGDQRSENWFN
                     STEVGGTASGSGLDMAQTAPDTEDVVANSLTVSGPTITFNGLSTDDGDETTNDLPDTW
                     TVGAIVELIVPDSYVVTNDGAYSRITSDTLEEIAPYVGMPVTLWYNSIDYQLFIADFI
                     PHSEPVGEDVITASITLAYDSATGTPFTGIPEGYVRLSVSHSGSEYKILDIDGSSVTL
                     ERVIDGSVDPSWPGFSPRTVLDFEANGLNENDSWMGPFLACPENEVVDMFEVNFFFPN
                     GICGYNKKGNKQYREVKWEIQYRPYGSGVGWISKTGSYYLQNINGLGFTERITLDTPA
                     LVEVRARRTNEQGQNNSRDNMYWQSLRGRLLSRPASYTGVTTIAVTVETGGKLAAQSD
                     RRVNVVATRVYDSGVSRSISGALYHIGNELGLAMDREAIDSLETTYWTPGSEFFDYAT
                     TDSVSALEMLQKVTNAGKSYFLLTDGLASVAREGVKTWTGIISPQEMTDPLQTAFVAP
                     SADDYDGVDVTYINGTTWAEETVQCRTSDNPTPVKIEDYTLDGVLDQDRAYQIGMRRL
                     MKYLQQRLTHTTTTEMDALCYNVGDRVVFTDDIPGSKTISTLIEDMSTSDGVTTITVS
                     EPLDWTFNNPRALIRYQDGSASDLLAATRVNEYVLSVIEQAKFSNIMLNAPSIESPRL
                     IFCESSRVGYSALISEIAPQSDGTCQVTAKEYRDTFYQYDNATYPGNVA"
     gene            complement(3204..3584)
                     /locus_tag="ACHKFK_004198"
     CDS             complement(3204..3584)
                     /locus_tag="ACHKFK_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016160832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter"
                     /protein_id="HFO9054418.1"
                     /translation="MFNPDKYLSVILQKGGRTYPELDCFGIVNEVRKDLGLPLWPDFS
                     GITKDGGGLNREARKLMLSLEKCAPCIGAGAACYSGSTVTHVGVVVEINGQLHVAECN
                     PGVNVTFLPVSRFKRRFVKVEFWK"
     gene            complement(3594..4076)
                     /locus_tag="ACHKFK_004199"
     CDS             complement(3594..4076)
                     /locus_tag="ACHKFK_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1833 family protein"
                     /protein_id="HFO9054419.1"
                     /translation="MTILNRLYASSGEEVIIETLQINIGTGVYYLCKGFDDITATTES
                     GVVVTFQAAAIDIALPARNSDGTQDLQFAIDNIDGITSTAIRNALDNLSEASLTYRNY
                     VSTDLSAPASVPYTLAIKSGSWTSMQAQITAGYMNVLDTAWPRHRYTLPYYPGLRYMS
                     "
     gene            complement(4073..4546)
                     /locus_tag="ACHKFK_004200"
     CDS             complement(4073..4546)
                     /locus_tag="ACHKFK_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054420.1"
                     /translation="MAKTTSINYPNDYLPVPLQEGFGLKPVSPLLRTELTSGRARQRR
                     LYTSTPTQASVAWLLTDPEAQLFEVWFRDTIKDGADWFNMPLRSPLGVVDVYVCRFVD
                     IYEGPTIEGGNYWRYTATLELWERPVLAPGWADFPDYIINSSIIDIALNREWPEP"
     gene            complement(4546..7071)
                     /locus_tag="ACHKFK_004201"
     CDS             complement(4546..7071)
                     /locus_tag="ACHKFK_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9054421.1"
                     /translation="MAGKSLGTLTIDLIAKVGGFVSGLSQAERASQKWRKQVQADAKA
                     AAVAFTGFATAASAAAIGVGVAGYNLLKTTSKQITETDRWAKSLNMSTQSLLAWQYAA
                     EKAGVSGDQMADIFKDIGDKIGDAVLNKSGEAVDALNALGLSAKKLSSESPDKQLLAI
                     SNALGKIKTNAEKTTILESLGNDLSKLLPLLDQGGEKLRQYMDAAKQFGVAPDDADIE
                     KLVKVNSLFEDMETQVNGVKIEIATGLANVDLSGLQNAITDMGDVFKDPQVIQGLTDL
                     VGGVVDLATWLVKVGAEAGKLIDLYKGGKAVGDNASVTDIERRLNNLKTDVEDQGFLA
                     SFNRIGMDVDGKKAEIAQLERRLSIMKAGNNLPLSQATVGAPSSSSKNYSLGSGETNG
                     KASPDAGARKLESAFKSLEMSYQRQIALIDTTGKKNQQVTELEKLRFDFTSGKLTGIN
                     AAQKERLEQLATEIDRLNSLKKANEENLKLVEFTANLRKQNQNDQAANDSDFIGAGMG
                     DKTRQRMKELLDIHRSFLDRQQDLQKQYQSGDISKSLYDQETAALQQALDDRLDIQED
                     YYKKSDAQMGDWQSGIMDSLNDYADKSADYYQTAADAMTSILGGATSVISDNLNDLVH
                     GAEDLGDFFSNIFSGLGETIIKTLSDMAAQWLVYQAVQLLVGKSTQASAAASMLANAQ
                     ASSLQAQIAAYASTAAIPIVGPALAPAAMATAAAVTAPLVAAVGTSALAGMAHDGIDS
                     VPETGTWLLQKGERVVTSQTSAKLDETLDRVNQQSTSGASFSPVINMNVNGDPSDTQI
                     SMMKQAMAEGAKLGYQQAASDLASGKGDISKAMMRWNTNRRTG"
     gene            complement(7339..7716)
                     /locus_tag="ACHKFK_004202"
     CDS             complement(7339..7716)
                     /locus_tag="ACHKFK_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone family protein,
                     TAC"
                     /protein_id="HFO9054422.1"
                     /translation="MKLTLDSLKQAGAFTGRPVEKEITWKQSDKEITATVYIRPMGYH
                     DAVSTVLSAAGKIDGVAGRIASSICDENGNPVFTVADITGEADPERGALDGNLTVALL
                     VAIQQVNDLGKTNSAQKTNSGVN"
     gene            complement(7769..8266)
                     /locus_tag="ACHKFK_004203"
     CDS             complement(7769..8266)
                     /locus_tag="ACHKFK_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO9054423.1"
                     /translation="MSVVTQGTQMYVLNNGVVSEVECITSFSPGSSPADQIEDTCLSE
                     TNTRSYKKGLRTPGQATVALNADPANASHVMLSNLAESSDQTNLTFAIGWADGTADPT
                     AATVGDPDAVDGLSLPDTRTWYVFQGYVSDFPFDFQANTVVQTSATIQRSGQGVWVPK
                     AQPTS"
     gene            complement(8319..8684)
                     /locus_tag="ACHKFK_004204"
     CDS             complement(8319..8684)
                     /locus_tag="ACHKFK_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFO9054424.1"
                     /translation="MIAPIFPVCASSPEVTTLLGSNPVRIYPFGIQDDNVVYPYAVWQ
                     NVSGSPENFLNQRPDADIYSLQVDIYADTPDEAIAVAQAMRNAIEVKANIVRWGNQTR
                     DPETLRYRYSFDVDWIVNR"
     gene            complement(8681..9166)
                     /locus_tag="ACHKFK_004205"
     CDS             complement(8681..9166)
                     /locus_tag="ACHKFK_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003833948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO9054425.1"
                     /translation="MVDSIDFKLEGVDSLLGKLEAITSETKRKTGRSALRKAGNVIVT
                     QIKRNAERLDDPHTARSIADNAALRWNGRMFKQTGDLAFRIGILQGAVLKKHPSTAKD
                     APTPHWRLLEFGTEKMAAKPLVRAAANSRLIEVFNTFSVNYEAGIDRAIKRAQKKGET
                     A"
     gene            complement(9159..9491)
                     /locus_tag="ACHKFK_004206"
     CDS             complement(9159..9491)
                     /locus_tag="ACHKFK_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO9054426.1"
                     /translation="MEFAKLRHRITIQRRTATQSPTTGAMEYTWNDLAEVWGNVVASS
                     VRDFITAQASNVKVTARITIRYREDIQEKDRILFRGKIYSIEGILPDPDSGLEYLTLP
                     CSEGVRDG"
     gene            complement(9494..9688)
                     /locus_tag="ACHKFK_004207"
     CDS             complement(9494..9688)
                     /locus_tag="ACHKFK_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006811056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054427.1"
                     /translation="MNEQQVNELTAALKALATSQLKQAEAINRLAQADETLISLIAKT
                     LVDEIDDELPPQTYLDGKPR"
     gene            complement(9759..10085)
                     /locus_tag="ACHKFK_004208"
     CDS             complement(9759..10085)
                     /locus_tag="ACHKFK_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO9054428.1"
                     /translation="MIELVTLEEAKLHLRIDDDYGDSDLTLKIQGGSAAILSYIQGSR
                     ALVVDDSGKLIDGEPLTRVQTALLVLLGYLDRNRGGEEEEKLKQGELPFSVSMLIYDL
                     RKPTII"
     gene            complement(10082..>10409)
                     /locus_tag="ACHKFK_004209"
     CDS             complement(10082..>10409)
                     /locus_tag="ACHKFK_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007898363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HFO9054429.1"
                     /translation="HEKTHGMRGWECIVDGVSGFDIKQDNQRTFSISLERASGTVEVK
                     SFDIPVTIYRDVFKSGTEYQPGDTVTWGGCMWHCNEKTCDKPGETGSKGWTLAVKKGR
                     DLRDKP"
BASE COUNT         2616 a         2642 c         2319 g         2832 t
ORIGIN      
        1 atgtcaggac agataccgat atatttttca ccatctaagc tgagtagatc tccacgcaat
       61 gaagcatcac ccacgctaac ccacgcacct aatccaatcc cacctgtcgt ttctggcgta
      121 gagccagcag gaacggtttt aggtaataca ccatcccaac ggtaatactc gccagaggtt
      181 tcgtcgtgca atgcctgatt agtcaaggtt atctctgcgc caacctgaaa tgattttatt
      241 agtatccaac ccatttttga caatgattct ttgtactttg actccatacc atgccaagta
      301 agacgtggat taccaaaacg atcaggccat aactcattgg attgatcatt agaaagatga
      361 tcgaagttct gggcgttatc gtacaaatct tttgcagcag aagaccccag cggattgcca
      421 gtattataag tcgtcatatt tgcctcataa gagaaaaacc cgccgtagcg ggtaatattg
      481 atgtttggat taagctacat tgccggggta ggtagcgtta tcgtactgat agaaagtgtc
      541 gcgatactct tttgccgtta cctgacatgt tccatcagat tgcggtgcta tctctgaaat
      601 taatgcgcta tatccaacac gggatgactc gcagaaaatc agacgaggcg attcaatgga
      661 tggggcgttt agcatgatat tgctgaattt tgcttgttct attactgata gaacatattc
      721 atttaccctt gtagctgcca gcaaatccga agcagaacca tcctgatagc gtatcaatgc
      781 acgtgggttg ttgaatgtcc agtcaagtgg ttctgatact gtaatagtcg ttacgccatc
      841 acttgtactc atatcctcaa tcaacgtgct tattgttttg ctaccaggaa tatcatccgt
      901 gaatactacc ctgtcgccaa cgttataaca gagagcatcc atttccgttg ttgtggtatg
      961 agtcaaacgt tgctgaaggt acttcatcag acgtcgcata cctatctgat aagcacggtc
     1021 ctggtcaaga acaccatcaa gcgtatagtc ctcaatcttt accggagttg ggttatctga
     1081 tgttctgcac tgcaccgtct cttcagccca tgtagtgccg ttgatatatg taacatccac
     1141 accatcatag tcatctgctg acggagcaac gaaagcagtc tgaagcggat cagtcatctc
     1201 ctgtgggctg attattccag tccacgtttt cacaccttcc cttgctacag atgccagacc
     1261 atcggtaaga aggaagtaac tttttcctgc atttgtcacc ttctgaagca tctccagcgc
     1321 agaaacagaa tccgtagtgg catagtcaaa gaattcactg ccaggggtcc agtaagtagt
     1381 ttctagtgag tcaatagctt cacggtccat cgctagcccc aactcattac ctatatgata
     1441 cagagcacca gaaatgctcc ttgacacacc agaatcataa actcgcgtgg ctacaacgtt
     1501 tacgcggcga tctgactgcg ctgccagttt accgccagtt tccacagtaa ccgcaattgt
     1561 ggtgacgcca gtatacgatg ctggccgtga caacaaacgt cctcgcaatg actgccagta
     1621 catgttatcg cgactgttat tctgtccctg ctcgttcgtg cggcgagctc tcacctctac
     1681 tagcgcaggc gtatccagcg tgattctttc ggtaaatccc agtccgttaa tgttctggag
     1741 gtagtacgat ccggttttac ttatccaccc gacgcctgaa ccatatggcc ggtactgaat
     1801 ctcccacttt acctcacggt attgtttgtt cccttttttg ttatacccac aaatcccgtt
     1861 tgggaagaag aagtttactt cgaacatatc aacaacttca ttttctgggc acgccagaaa
     1921 aggtcccatc caactatcgt tctcatttaa accgttggct tcaaaatcaa gaactgtgcg
     1981 cggtgaaaat ccaggccatg acggatcaac gctgccatca ataacccgct ccagagttac
     2041 agagctacca tctatatcaa gaatcttata ttcgcttccg gaatgggaaa cagatagcct
     2101 gacataacct tcaggaatcc cggtgaacgg agttccagtg gcgctatcgt aagcgagggt
     2161 tattgatgcg gtaatcacat cttcgccgac tggctcagag tgaggtataa aatcagcaat
     2221 gaatagttgg taatcaatgc tgttgtacca gagagttaca ggcatcccaa cataaggagc
     2281 tatttcctcc agcgtgtcac tggtaattct gctgtaagca ccatcattag ttaccacata
     2341 ggaatcagga acaattaact caacaattgc accaacagtc catgtatctg gaagatcatt
     2401 tgttgtttca tctccatcat ccgtgctcag cccattaaat gttattgttg gtccggaaac
     2461 ggttagcgaa ttagcaacaa catcttccgt gtccggcgcg gtttgcgcca tatcaagacc
     2521 agaaccagat gcggttcccc caacctctgt ggaattgaac cagttttctg atcgttgatc
     2581 tccactaacg ctttgcccgg gttgatacac tgtgtagtta aacccatcac caagagaagc
     2641 aattggagtt gaccctacac gaatatcgcc atccccaaat gaaaaacgcc ctctccccat
     2701 aacaacaaac atttcaactg tcattaccgt tggatcatca actgagaatc ttgttacagg
     2761 ctgcactgca tagtcaggat aaatacggct tcgtccaaat aactcacgta tcgggtcgcc
     2821 caattttgcg ttattcgctt tagcaggatt tacatcaaga gggtttcccg ttcctgatga
     2881 ataaccgcca aggtcaccaa caccaggagc aaagaaaaga gcatacgcga ctgatgcaac
     2941 tgatacagca acggcaatcc aggcaagagc tacagcgcca taagggacag ggtaaattct
     3001 gacatcactg tctgatttga tagcaaattc aaaccatgcc tgaggcggta tgttctctcc
     3061 atcaacatca acggtgatcg ggtgcttcat atcaggctta tagccctgaa cgttcttcgc
     3121 aagccactga tggatagtta ctgcgccatg ttcatgagtt tcgagtggct cgccaggcag
     3181 ccgtgaagga taaattctga tagtcacttc cagaactcca ctttgacaaa acgacgtttg
     3241 aaacgcgaga caggaaggaa ggtaacgttc actcctgggt tgcactctgc gacatgcaat
     3301 tggccgttaa tttcaacgac tacaccaaca tgagtaacgg ttgacccgga ataacaggcg
     3361 gcaccggcac ctatacacgg cgcgcatttt tccagagaaa gcatgagctt tctggcctct
     3421 cggtttaaac ctccgccgtc ttttgttata cctgaaaaat ctggccacag tggtagcccc
     3481 aggtcttttc gtacctcgtt cactatgccg aagcagtcaa gttcaggata agtgcgaccg
     3541 cccttctgca atatgacaga aaggtattta tcaggattga acattgttgc tccttaactc
     3601 atgtaacgaa ggccggggta atacgggagg gtgtatcggt ggcgcggcca tgcggtatca
     3661 agaacgttca tatatccggc agtaatctgc gcctgcatgg atgtccatga cccgctttta
     3721 atcgccagcg tgtacggtac agatgccgga gcactgaggt ccgtggagac atagttacga
     3781 tatgtcagcg atgcctctga aaggttatcc agagcattcc gtatggcggt ggaagtaatc
     3841 ccgtctatgt tatcaatggc aaactgcaaa tcttgtgtac catcgctgtt tcttgctgga
     3901 agggcaatat caatagccgc agcctgaaag gttaccacca cgccactttc ggttgttgcg
     3961 gtgatgtcat cgaagccttt acaaagataa taaacaccag taccgatgtt tatttgcagg
     4021 gtttcgatga tcacctcttc cccggatgag gcatacagcc tatttagaat cgtcatggct
     4081 caggccactc cctgttaagc gcaatatcaa tgatgctgct gttgataatg taatcaggaa
     4141 aatcagccca gccaggagcc agaacaggcc tttcccacaa ttccaacgtg gccgtgtagc
     4201 gccagtagtt tccgccttca attgtcggcc cctcatagat atccacgaaa cgacagacat
     4261 aaacatcaac aacgcctaac ggagatcgaa gaggcatgtt aaaccagtca gcgccatcct
     4321 taatggtgtc cctgaaccat acctcaaata gttgagcttc cggatccgta agcagccatg
     4381 caaccgaggc ctgagttggc gttgaagtat ataatctcct ctgcctcgcc ctgccggatg
     4441 tgagttcggt tctcagtaat gggctaactg gctttaaccc gaacccttcc tgcaatggaa
     4501 caggaaggta atcattcgga tagttaatgc tggtcgtttt agccattaac cagttctcct
     4561 gttagtgttc cagcgcatca ttgctttcga aatgtcacct ttcccactgg caaggtcgct
     4621 ggctgcctgt tgatatccaa gttttgcccc ctctgccatg gcttgcttca tcatggagat
     4681 ctgagtatca gacggatcac cgttaacatt catgttaata acgggagaga agcttgctcc
     4741 gctggttgat tgttgattta ctctgtccag tgtttcatca agctttgctg acgtctgaga
     4801 ggtcacaact cgctcacctt tctggagcaa ccatgttcct gtttccggga cgctatcaat
     4861 accatcgtgc gccatacctg ccagggcaga agtaccaact gctgcaacaa gtggtgcagt
     4921 tactgccgca gctgttgcca tcgctgcagg tgcaagcgcg ggaccaacga taggaattgc
     4981 cgctgtcgat gcgtatgctg cgatttgagc ctgcaaagat gatgcctgtg cgtttgccag
     5041 cattgatgcc gctgcgcttg cctgagtgga tttacctacc agcaattgca ctgcctgata
     5101 taccagccat tgcgccgcca tatcagaaag ggttttaatg attgtttcac caagaccaga
     5161 gaaaatatta ctgaagaaat cacctaaatc ttctgcaccg tgcacaaggt cattaaggtt
     5221 gtcagaaata accgaggttg ctccaccaag aatagaagtc atcgcatctg cagcagtctg
     5281 gtaataatca gctgatttat cagcataatc gttcaacgag tccattatcc cgctttgcca
     5341 gtcgcccatc tgagcatcag actttttgta gtagtcctcc tgaatatcaa gacggtcatc
     5401 aagcgcctgc tgtaacgctg ccgtttcctg gtcataaagc gatttgctta tatcaccact
     5461 ttggtactgt ttctgtagat cttgctgcct gtcgagaaaa ctacgatgga tatccagcaa
     5521 ttccttcatg cgctggcgag tcttgtctcc catacctgcg ccgataaaat cagaatcatt
     5581 agctgcctgg tcattctgat tttgcttacg taaattagcg gtaaattcga caagtttcag
     5641 attttcttcg ttggcttttt taagtgagtt gaggcgatct atctcagtag caagttgctc
     5701 aagtcgctct ttctgagctg cattaattcc tgttaatttc ccagatgtaa aatcaaaacg
     5761 cagcttctca agctcggtta cctgctgatt ctttttgcca gttgtgtcaa tcagcgctat
     5821 ctgacgctga tagctcattt ccagagactt aaatgctgac tccagttttc tggccccggc
     5881 atctggagat gctttcccgt tagtttctcc tgagcctaag gaataatttt tgctggaaga
     5941 agaaggcgca cctacagtgg cctgactgag aggaagatta ttaccggctt tcatgatgga
     6001 cagtcggcgt tctaattgcg ctatttcagc ttttttcccg tcaacgtcca taccaattcg
     6061 gttaaagctg gcaaggaatc cctgatcttc tacatcagtt ttaagattgt tgaggcggcg
     6121 ctctatatca gttacagatg cattgtcacc tacagcctta ccgcctttgt acaggtcaat
     6181 tagcttccct gcttcagcac caactttaac aagccaggtc gcgagatcaa caacaccacc
     6241 aaccaggtct gtcaaaccct gaataacctg aggatctttg aatacatccc ccatgtcagt
     6301 tatcgcgttc tgtaaccctg acaagtcaac attggccagg ccggtggcaa tctcaatttt
     6361 tacaccgttt acctgcgtct ccatgtcctc aaacagggag tttactttta ccagcttttc
     6421 gatatcagca tcgtcagggg caacgccaaa ctgttttgcc gcgtccatgt actgacgaag
     6481 tttttctcct ccctgatcaa gcaatgggag aagcttggaa aggtcgttac caagactttc
     6541 aaggattgtg gtcttttcag cgtttgtttt aatcttgccg agcgcattgc tgatagccag
     6601 caactgctta tctggtgatt cactggataa tttcttagcc gataatccga gtgcattcag
     6661 agcatctact gcttcacccg atttattcag tacagcatcg ccaattttat cgccaatatc
     6721 cttgaagata tcggccatct ggtcgccgga tacgccagct ttttcagctg catactgcca
     6781 ggcaagcaat gactgcgtag acatattgag cgacttagcc caacggtctg tttcggtaat
     6841 ctgttttgac gttgttttta gcaggttgta accggcgaca ccgacaccga tagctgccgc
     6901 acttgccgcg gtagcaaaac cagtaaatgc taccgcagcc gcttttgcat cagcctgaac
     6961 ctgttttcgc catttttgag atgctcgttc agcctggctt agaccagata caaatccacc
     7021 aaccttagct atcaggtcaa tggtcagcgt tccgagggat ttaccagcca tgctatgtcc
     7081 actcctgcat agcctgctcc agtgaaatgg aaggctcgtt aatgtgtggt gtgaagtctg
     7141 ttactttgaa ggagggggaa tcttttccac ggttgacgtt agccagcaca gaagaaataa
     7201 ggcctgcggc ccactcagta cgcatcattc cgttaaggct accgtacttt tgacggtaaa
     7261 gaacccagtc gcgatactct gtaacgctaa cgcgttcttt tgcttcagca atggtgcgac
     7321 cgccgatccc gttgagaact aattcacacc agaattcgtc ttctgcgctg agttcgtctt
     7381 tcccaggtcg ttaacctgct gaatagcgac aagtagagcg acggtaaggt ttccgtcgag
     7441 cgcaccgcgc tcagggtctg cttcaccagt aatgtcagca acagtaaaaa caggattccc
     7501 gttctcatca caaatggatg atgcgatacg tccggcaaca ccatcaatct ttcccgcggc
     7561 tgacagaaca gtagatactg cgtcgtgata acccataggt cggatataaa cggtagcggt
     7621 aatttcctta tcactctgct tccaggtaat ctccttctca acaggtctcc cagtgaatgc
     7681 accagcctgt tttaatgaat ccagagtcag tttcatttat tactctctta ttgcggggtt
     7741 acccccgcta gttggcagat tattacgttt agctggttgg ctgtgcttta ggaacccaga
     7801 cgccctggcc ggaacgctga atagtcgcgg aagtctgaac taccgtattg gcctgaaaat
     7861 caaacgggaa atctgataca taaccctgga atacatacca ggtacgggta tctggaagag
     7921 acagaccatc aactgcatca gggtcaccga ctgttgcagc agtaggatct gcagtaccat
     7981 cggcccagcc tatagcgaag gtcaggttgg tctggtcgct tgattcagcc aggttgctca
     8041 gcattacgtg actggcgttt gccgggtcgg cgttaagagc aacggttgcc tgacccgggg
     8101 tgcgcagacc ttttttataa gaacgggtat tggtttcgct cagacaggta tcttcaattt
     8161 gatctgccgg gctactacct ggtgagaatg aagtaataca ttcaacttca ctgaccacgc
     8221 cgttattcag aacgtacatc tgagtgcctt gagtcactac tgacataatt atctccgggt
     8281 ataaaaaaac cggctcaagg ccggttgtgg aaggtttgtt atcggttgac tatccagtca
     8341 acgtcgaaag aatatcgata cctgagcgtc tcagggtctc gcgtctgatt accccagcga
     8401 acaatgttgg cttttacctc aatcgcatta cgcatggctt gagctacagc aatcgcctca
     8461 tcaggggtat cggcatagat atcaacctga agcgaataaa tgtccgcatc tggtcgttgg
     8521 ttaaggaaat tttcaggaga gccgctgacg ttctgccaga cggcgtatgg ataaacaaca
     8581 ttatcgtcct gaattccgaa agggtaaatt cttaccggat tgcttccgag taaagtagtg
     8641 acttcaggac tcgacgcgca aacaggaaaa ataggagcaa tcatgccgtc tctcctttct
     8701 tctgtgctcg tttgatagct cggtcaatcc ccgcttcata gttaacagag aaggtgttga
     8761 aaacctctat cagtctggaa tttgcagcag cacgaacgag cggttttgct gccatctttt
     8821 cagtgccaaa ctcaagaaga cgccagtgag gagtgggggc atctttcgca gtgcttggat
     8881 gcttttttaa aacggctccc tgaagaatgc ctatcctgaa ggcaagatca ccggtttgtt
     8941 taaacatacg accattccag cgcagcgcgg cgttatcagc aatgctacgt gcggtgtgag
     9001 gatcgtcgag tcgctctgcg tttctcttta tctgagttac gataacgttt cctgctttcc
     9061 tcagtgcgga gcgccctgtt ttgcgcttag tttctgaggt aatggcttct aacttaccaa
     9121 gcagtgaatc tacaccttca agcttaaaat caatgctatc aaccatccct taccccctct
     9181 gagcacggaa gcgtaagata ttcgagccca ctatcagggt caggaagaat cccctcaatg
     9241 ctgtagattt tgccacggaa aagaatacgg tctttctcct ggatatcttc ccggtatcgg
     9301 atagtgattc tggccgttac ttttacgttt gatgcctggg ctgtaatgaa atccctgacc
     9361 gatgaggcaa ccacgttacc ccacacctct gcaaggtcat tccaggtgta ttccattgcc
     9421 cctgtggttg gtgactgggt ggctgttcgt cgctgaatag tgatgcggtg acgcagttta
     9481 gcaaactcca cgattacctc ggcttaccat caagataggt ctgcggtggt agctcatcat
     9541 caatttcatc aacgagcgtt ttggcaatca gagatatcag tgtttcatcc gcctgagcca
     9601 gacggtttat cgcttctgcc tgtttcagtt gagacgttgc cagcgctttc agtgccgccg
     9661 ttagttcgtt tacctgctgc tcgttcatag gcaatcctcg tccacttttt aatccactcg
     9721 cgccgtctgg cacatcctgc gcatgccata catgcccctt aaataattgt cggcttacgg
     9781 aggtcgtaaa tcagcattga tacagaaaaa ggaagttccc cctgtttcaa cttttcttct
     9841 tcttcgccgc cgcggttacg gtcaagatag ccaagaagaa ccagcagggc cgtctgaacg
     9901 cgagttagcg gttcaccatc aataagcttt cctgaatcat ccacgactaa cgcgcgactt
     9961 ccctgaatgt aggaaagtat tgctgcgctg ccgccctgaa tttttaaggt aaggtctgaa
    10021 tctccgtaat cgtcgtcaat acggagatgc aacttagcct cttcgagcgt caccagttca
    10081 atcatggctt atccctcaga tctcgccctt tcttaacagc aagcgtccat cctttcgatc
    10141 ctgtctcacc cggcttgtcg catgttttct cgttgcaatg ccacatacaa ccaccccatg
    10201 tgaccgtatc gccaggttga tattcggtac cagatttgaa tacatcgcga tagatagtta
    10261 ccgggatgtc aaaggactta acttcaacag taccgcttgc tctttcgaga gaaattgaga
    10321 aggttcgctg gttatcttgt ttgatatcaa agcccgatac accgtcaaca atacattccc
    10381 agccccgcat accgtgcgtc ttttcatgg
//
LOCUS       DAZGJT010000136        10248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZGJT010000136 DAZGJT010000000
VERSION     DAZGJT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.138"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..1774
                     /gene="ydeN"
                     /locus_tag="ACHKFK_004210"
     CDS             92..1774
                     /gene="ydeN"
                     /locus_tag="ACHKFK_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO9054430.1"
                     /translation="MKSALKKSIVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPEQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQNKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDNSQPPLSEVNQEKFNNIKKALSKAK"
     gene            1826..2983
                     /gene="ydeM"
                     /locus_tag="ACHKFK_004211"
     CDS             1826..2983
                     /gene="ydeM"
                     /locus_tag="ACHKFK_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO9054431.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQIEFNTLTVI
                     NNINVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMRWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCVYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2949..3074)
                     /locus_tag="ACHKFK_004212"
     CDS             complement(2949..3074)
                     /locus_tag="ACHKFK_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054432.1"
                     /translation="MKTKHPTGIYVGCLVKVIRRRLRMELRGSVINYSPFVLQHP"
     gene            3274..4959
                     /gene="yddA"
                     /locus_tag="ACHKFK_004213"
     CDS             3274..4959
                     /gene="yddA"
                     /locus_tag="ACHKFK_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO9054433.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWSIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSENFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALSLSVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLVDDICDISAVI"
     gene            4997..7369
                     /gene="yddB"
                     /locus_tag="ACHKFK_004214"
     CDS             4997..7369
                     /gene="yddB"
                     /locus_tag="ACHKFK_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO9054434.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPESDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDISGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAARKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHISQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNRVVYNGNLVSYDSVPVTDFNNPLKISLNMDFT
                     HQPSGLVWANTLTWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7426..10209
                     /gene="pqqL"
                     /locus_tag="ACHKFK_004215"
     CDS             7426..10209
                     /gene="pqqL"
                     /locus_tag="ACHKFK_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO9054435.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNEVAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTFSNGARVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLPQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSNSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYNSRTPVNLATRMALDAFNVALAKDLRINIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
BASE COUNT         3005 a         2265 c         2252 g         2726 t
ORIGIN      
        1 tatctattta tgtgactaac ttcacttaca ttaacttatt tctcttcgta aaattacttt
       61 ggaattaagt acaataagaa gaggaacatt tatgaagtct gcattaaaga aaagtatcgt
      121 aagtacctcg atatctttga tactggcatc tggtatggct gcatttgctg ctcatgcggc
      181 agatgatgta aagctgaaag caaccaaaac aaacgttgct ttctcagact ttacgccgac
      241 tgaatacagt accaaaggaa agccaaatat tatcgtactg accatggatg atcttggtta
      301 tggacaactt ccttttgata agggatcttt tgacccaaaa acaatggaaa atcgtgaagt
      361 tgtcgatacc tacaaaatag ggatagataa agccattgaa gctgcgcaaa aatcaacgcc
      421 gacgctcctt tcattaatgg atgaaggcgt acgttttact aacggctatg tggcgcacgg
      481 tgtttccggc ccgtcccgcg ccgcaataat gaccggtcga gcgcccgccc gctttggtgt
      541 ctattccaat actgatgctc aggatggtat tccgctaaca gaaactttct tgcctgaatt
      601 attccagaat catggttatt acactgcagc agtaggtaaa tggcacttgt caaaaatcag
      661 taatgtgcca gtgccggaag ataaacaaac gcgtgactat catgacaact tcactacctt
      721 ttctgcggaa gaatggcaac cgcaaaaccg tggctttgat tactttatgg gattccatgc
      781 tgcaggaacg gcatattaca actccccttc actgttcaaa aaccgtgaac gcgtccccgc
      841 aaaaggttat atcagcgatc agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa
      901 aacacttgac cagcctttta tgctttacct ggcttataat gctccgcatc tgccaaatga
      961 taatcctgct ccggagcaat atcagaagca atttaatacc ggtagtcaaa cagcagataa
     1021 ttactacgct tccgtttatt ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa
     1081 gaaaaacgga cagtatgaca atacaattat tctctttacc tccgataatg gtgcagttat
     1141 cgatggccct ctgccgctga acggggcgca aaaaggctat aagagtcaga cctatcctgg
     1201 cggtactcac accccaatgt ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga
     1261 caagctgatt tccgcaatgg atttctaccc gacagctctt gatgcagccg atatcagcat
     1321 tccaaaagac cttaagctgg atggcgtttc cttgctgccc tggttgcaaa ataagaaaca
     1381 gggcgagcca cataaaaatc tgacctggat aacatcttat tctcactggt ttgatgagga
     1441 aaacattcca ttctgggata attaccacaa atttgtccgc catcagtcag acgattaccc
     1501 gcataacccc aacaccgagg acttaagcca attctcttat acggtgagaa ataacgatta
     1561 ttcgctcgtc tatacagtag aaaataatca gttaggtcta tacaaactga cggatctaca
     1621 gcaaaaagat aaccttgccg ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt
     1681 aagagagttt atcgataaca gccagccacc gcttagcgag gtaaatcagg agaagtttaa
     1741 caatatcaag aaagcattaa gcaaagcgaa ataactaaac cttcatgcgg cggatttttc
     1801 cgccgcctta ttgagcgaga tagcgatgca cgttacagcc aagccctcca gttttcaatg
     1861 taatctcaaa tgtgattact gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa
     1921 atggatggat gacagcactc tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa
     1981 tcaggtctat tttacctggc aaggcggtga acccactctg gctggcctgg attttttccg
     2041 taaagttatt cactatcaac aacgctatgc aggccaaaaa cgaattttta atgcattgca
     2101 aacgaatggc attttattga ataatgaatg gtgtgccttt ctcaaagaac atgagtttct
     2161 ggttggtatc tcgatcgatg gaccccaaga actacatgac cgctacagac gcagtaattc
     2221 aggtaacggt acttttgcaa aagtgatagc agccatcgag cgtctgaaat catatcaaat
     2281 agagtttaat acgttaactg tcattaataa cattaatgtc cattaccctc ttgaggttta
     2341 tcatttttta aaatctatcg gcagtaaaca tatgcaattt atcgaattgc tcgaaaccgg
     2401 gacgccgaat attgatttca gtggtcatag tgagaacaca ttccgtatca ttgatttttc
     2461 tgtgcctccc acggcttatg gcaagtttat gtcaaccatt tttatgcgat gggttaaaaa
     2521 cgatgtgggt gaaattttca tccgtcagtt tgaaagcttt gtcagccgtt ttttggggaa
     2581 tgggcatacc agttgtattt tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa
     2641 tggagacatt tacgaatgcg accattttgt ctatccacag tacaaaattg gaaacattaa
     2701 taaatcggaa ctcaaaacga tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc
     2761 agccaaatgt cagcaatgtg tatataaacc tatctgcaat ggtggttgcc ctaagcatcg
     2821 tattactaaa gtaaacaatg agactgtttc ctatttttgc gaaggttata aaatcctttt
     2881 ttcaaccatg gtaccttata tgaacgccat ggtggagtta gctaagaaca gagtaccgct
     2941 ttaccacatt atggatgttg caaaacaaat ggagaataat taataacgct cccccttagc
     3001 tccattctta gcctccttct tatgacctta acaaggcacc cgacataaat tcctgtcggg
     3061 tgtttcgtct tcatcatctt cctttgcgaa aatcctggct tacgaggtat gcgtcagggc
     3121 attagcaata cccttgatta tcaatagtat aaccataacc actgaaaatt aaatatttct
     3181 ttatatttca tattgttaaa tgatacttaa ataaatttca tcagtatatc attgccatag
     3241 ataatcattg ggtggcacaa tcaattttcg ctaatgataa ccattcccat tacgttttgt
     3301 atgttgatag caaagtatct ctgtctgctg aagccctttt ggctgcgtaa gaataataaa
     3361 acttcagtgt tgttaatcat tattatcctc gccatgatcc ttggcgtggt taaaattcag
     3421 gtctggttaa acgactggaa taatgatttt ttcaatgcgc taagccagaa agaaactgat
     3481 aagctttggc agcttgtcct ttggttccct gccctgctgg ggatctttgt attaatatcc
     3541 gtaaacaaaa catggttaat taaactactg actattcgct ggcgcgaatg gttaacagat
     3601 tactacctta atcgctggtt tgcagataaa aactactact tcacgcagat ctacggcgag
     3661 cataaaaata cagataaccc tgaccaacgt atcgctgaag acattctctt actgatcagc
     3721 aaaacgctca gcctttcatt tggcttcatc cagtcactta gcatgctgat cacctttacc
     3781 gttattcttt ggcaaagcgc gggtacgctc tcattcactg ttggtggaac tgaatggagt
     3841 atccaggggt atatggtcta taccgtcgtg ctcattgtga tcggtggaac tttatttacc
     3901 cataaggttg gtaaacgtat tcgtccactt aatgtggaaa aacaacgtag cgaagcgaca
     3961 tttcgaacta atcttgtgca gcataataag caggcagaac tgattgcgct aagcaatgct
     4021 gagtctctac aacgccagga gttgagcgaa aattttcata ccattaaaga gaactggcat
     4081 cgtttaatga atcgtcagcg gtggcttgat tactggcaga atatttattc gcgctcgctt
     4141 agcgttcttc cctactttct gttattgccg cagtttatta gcgggcagat caatctgggc
     4201 ggactgatga aatcgcgcca ggcatttatg ctggtatcga acaatttaag ctggtttatt
     4261 tataaatatg atgaacttgc tgaactggct gcggttatcg atcgcttgta tgagttccat
     4321 caactcaccg aacagcgccc tacgaataag cctaaaaatt gtcaacatgc ggtacaagtg
     4381 gctgacgcga gtattcgtac tcctgataat aagatcatat tagagaacct gaactttcat
     4441 gtttcgccag gcaaatggct attgctgaaa ggttactctg gtgcgggaaa aaccacactg
     4501 cttaaaacat tatcccactg ctggccgtgg tttaaaggtg atatttcttc tcctgctgac
     4561 agttggtatg tgtcacaaac accgttaatc aaaaccggct tactgaaaga gattatttgt
     4621 aaagcacttt ccctgtccgt agacgataaa tcgttgagcg aagtactgca tcaggttggt
     4681 ctggggaaat tggctgcgcg tattcatgat cacgatcgct ggggagatat tctttccagc
     4741 ggcgaaaaac aacgtatcgc cctggctcga ttaattttac gacgtccgaa atggatattt
     4801 cttgacgaaa ctacctctca tcttgaggaa caagaggcaa tccgcttact gcgtttagtg
     4861 cgagaaaaac tccccacaag cggcgtcatt atggttacac atcaacccgg tgtctggaac
     4921 ctggtcgatg atatttgtga cattagcgcg gttatataaa aacaataaaa gccctgctgt
     4981 acggagatat aaataaatga agcgagttct tattcctggc gtcattttat gtggcgctga
     5041 tgtggcgcag gccgtcgatg acaaaaatat gtacatgcat gtttttgaag agatgacggt
     5101 ctatgctcct gtccctgtac ccgtaaacgg caacacgcat tacaccagtg aaagcatcga
     5161 gcgtttaccg accgggaatg gcaatatcag cgatctgctg agaaccaacc ctgcggtacg
     5221 catggattca acgcaaagta cctcattgaa ccagggagat attcgcccgg agaaaatctc
     5281 tattcacggt gcgtcgccct accagaatgc ctatttgatt gacggtatta gtgcaacgaa
     5341 taacctgaac ccagcgaatg agtccgatgc cagtagtgca accaatatta gcgggatgtc
     5401 acaggggtat tatcttgatg tcagcttact ggacaatgtg acgctttatg acagttttgt
     5461 gccagttgaa ttcggtcgct tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa
     5521 cgctgatgat agcagcgtga aactgggtta tcgcactacg cgttcggact ggttaacatc
     5581 gcatatcgat gagaataaca agagcgcatt taatcaaggt tcttcaggaa gtacttatta
     5641 ctctccagat tttaaaaaga acttttatac cttgtcgttt aatcaggaac tcgctgataa
     5701 ctttggcgtt accgctggtt tatcgcgccg ccagtctgat atcacccgcg cggattatgt
     5761 ttcgaatgac ggcattgtcg ccggcagggc acagtataaa aacgttatcg atactgcatt
     5821 gagcaaattt acctggtttg ccagcgaccg ctttacccac gatttaacct taaaatatac
     5881 cggctccagc cgtgattata ataccagcac cttcccggag tctgatcgcg aaatgggtaa
     5941 taaatcctat ggtctggcat gggatatgga tacccagctc gcatgggcca aactgcgtac
     6001 caccgttggt tgggatcata ttagtgatta tacccgtcac gatcatgaca tctggtacac
     6061 cgaactttca tgtacatatg gtgatatttc agggcgttgc acccgtggcg gattaggaca
     6121 catttcccag gctgtagata attacacctt caaaacacgc ctggactggc aaaaattcgc
     6181 cgtgggtaat gtttcgcatc aaccctactt cggcgcggaa tacatctatt ccgatgcatg
     6241 gactgaacgc cataaccagt ctgaatccta tgtgattaat gctgccagaa agaaaactaa
     6301 ccatacaatt taccataaag gtaaaggtag tctgggaatt gacaattaca cgctgtatat
     6361 ggcggatcgc attagctggc gtaatgtgtc gttaatgccc ggcgtgcggt atgactatga
     6421 taactatctg tcaaaccaca atatctcccc gcgctttatg acggaatggg atatttttgc
     6481 tgatcaaacc tcaatgatta ctgcaggtta taaccgttac tatggcggga atattcttga
     6541 tatgggatta cgtgatatcc gcaatagctg gacggaatcg gtatcaggca ataaaaccct
     6601 gacgcgttat caggatttga aaacgcctta taacgatgaa ctggcaatgg gattgcagca
     6661 gaaaatcggt aagaacgtta ttgcgcgtgc aaactatgtt taccgtgaag cgcatgatca
     6721 aatcagcaaa agcagtcgta ccgacagcgc gactaaaacc accattactg aatataacaa
     6781 cgatggcaaa accaaaacac attcgtttaa cctcagtttt gaactggccg aacccctgca
     6841 tatcagccag gtagatatta acccgcaaat tgtctttagc tatatcaaga gcaagggcaa
     6901 cttgtcgtta aacaatggtt atgaggagag caataccggt gataaccggg tggtttataa
     6961 cggtaatctg gtctcttacg atagcgttcc agtgacggat tttaataatc cattaaagat
     7021 ctccttaaac atggatttca cgcatcaacc gagcgggttg gtgtgggcga atacgctgac
     7081 ctggcaagaa gcgcgtaaag ctcgcattat cctgggtaag acgaatgcgc aatacatcag
     7141 cgaatattca gattacaagc agtatgttga cgagaaactg gatagcagcc tgacctggga
     7201 cacccgcttg tcctggacgc cacaatttct gaaacaacaa aacctgacga tcagtgccga
     7261 tattctcaat gtactggata gcaaaaccgc tgttgataca acgaatactg gcgtggcgac
     7321 ctacgccagt ggccgtactt tctggcttga tgtcagcatg aaattttaat agttcggatt
     7381 ttactttacc gggtaaattc gcccggtttt cgcatggaga taattatgag aaacctctgt
     7441 ttcttactga cgttagtggc aactctgttg ctccccgggc gactgattgc cgccgcctta
     7501 ccgcaggatg aaaagttaat taccgggcaa ctggacaatg gcttgcgata tatgatttat
     7561 ccgcatgctc aaccaaagga tcaggtaaat ttatggctgc aaattcatac cggttcattg
     7621 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
     7681 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
     7741 ggtcgcgatg ttaatgccta taccagctat gacgaaacag tgtatcaggt gagtttgccg
     7801 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
     7861 gcaacctttg aaaaactcga agtagacgct gaacgcggcg taattactga ggaatggcgt
     7921 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggcaaat
     7981 acccgtaatt tagaccgtga acctatcggc ctgatggata ccgtcgccac ggtcacaccg
     8041 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttattgtg
     8101 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
     8161 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
     8221 tttaatatca tcaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
     8281 ccaatggtac aagtgaacga tgagcaaagc tttatcgaac aagctgaatg gagcatgttg
     8341 gttcagctgt tcaaccaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
     8401 tctggcggca ctgcgcgcag cgtcaaaatt gcacccgatt atcagtcgct atttttccgt
     8461 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
     8521 accattgatc agcatggttt ttctgctgaa gaactcgatg atgttaaatc tacccgcctt
     8581 acctggctga aaaatgcggt tgatcagcaa gctgagcgtg atttacgtat gctgaccagt
     8641 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     8701 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     8761 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgaagt tgccgccaaa
     8821 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     8881 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     8941 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacatt ttccaatggt
     9001 gccagggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     9061 tctaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggctaat
     9121 aaagcagtaa gcggaagcgg cgttggtgaa ctctcctctt ccagcctgaa acgctggagt
     9181 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     9241 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     9301 agcacgatta acgacaatat ttgggcatcg ctgcaaaatg ctcaaattca ggcgttgaaa
     9361 acgctcgacc agcgtccagc ggagaaattc gcccagcaga tgtatgagac gcgctatgct
     9421 gatgaccgca cgaaattacc gcaagaaaat cagattgcac agtttactgc cgcagatgca
     9481 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     9541 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggatc tatcaaacac
     9601 tctaattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     9661 actgtaaaag aacaaaatga accagtggca caggtttcac agtggaagcg ttataattcc
     9721 cggactcctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     9781 aaagatctac gcattaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     9841 ctctcggttg atcctcaggc caaagatatc agtcatttgc tggcttttac ttgtcaacca
     9901 gaacgacatg atgaactgtt aacgttagcg aatgaagtga tggttaagcg tctggccaaa
     9961 gggattagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
    10021 caacagcgta gcgttcaaca attagcgaat accattgtaa atagtcttat tcaatatgac
    10081 gatcctgcag catggactga gcaggagcaa ttgttgaaac aaatgacggt agagaatgtt
    10141 aacactgctg ttaaacaata tctttctcat ccggtgaata cctataccgg agtattattg
    10201 ccaaaataat cacagccccg tcaacacatc gttggcgggg attttatc
//
LOCUS       DAZGJT010000137         9930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZGJT010000137 DAZGJT010000000
VERSION     DAZGJT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.139"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(7..126)
                     /locus_tag="ACHKFK_004216"
                     /pseudo
     CDS             complement(7..126)
                     /locus_tag="ACHKFK_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            204..1298
                     /gene="ddlA"
                     /locus_tag="ACHKFK_004217"
     CDS             204..1298
                     /gene="ddlA"
                     /locus_tag="ACHKFK_004217"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO9054436.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYTIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1322..1534)
                     /gene="yaiZ"
                     /locus_tag="ACHKFK_004218"
     CDS             complement(1322..1534)
                     /gene="yaiZ"
                     /locus_tag="ACHKFK_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO9054437.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1794..2102
                     /gene="yaiY"
                     /locus_tag="ACHKFK_004219"
     CDS             1794..2102
                     /gene="yaiY"
                     /locus_tag="ACHKFK_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO9054438.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYEHLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="ACHKFK_004220"
     CDS             complement(2161..3255)
                     /gene="yaiW"
                     /locus_tag="ACHKFK_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO9054439.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="ACHKFK_004221"
     CDS             complement(3268..4488)
                     /gene="sbmA"
                     /locus_tag="ACHKFK_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO9054440.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4840..5997
                     /gene="ampH"
                     /locus_tag="ACHKFK_004222"
     CDS             4840..5997
                     /gene="ampH"
                     /locus_tag="ACHKFK_004222"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO9054441.1"
                     /translation="MKRSLLFSAVLYAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVIRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYIAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="ACHKFK_004223"
     CDS             complement(5998..6621)
                     /gene="iprA"
                     /locus_tag="ACHKFK_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO9054442.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6707..9655)
                     /gene="ehaB"
                     /locus_tag="ACHKFK_004224"
     CDS             complement(6707..9655)
                     /gene="ehaB"
                     /locus_tag="ACHKFK_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO9054443.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVNVFNIGTAITLDQEVDLSISNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYNGDGWNADDVALAVIAHPYAD
                     NAMQTTATFDNSTLMGDVFFSSNFDENFFPQGRDSYRDADGDVDTNGWDGTDRLDLTL
                     NNGSKWVGAAMSAHQIGVDTDDDGVNDSYTYGINTEATATLIDIAANSLWPSSTVGVE
                     NSDSEYSEFDHIIGNEVYQSGLFNVTLNTGSQWDTTKTSLIDTLSINSGSTVNVADST
                     LISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEVSAGWAVGDAALYANNIKV
                     TNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNIESNRFVLDADLTNDRTND
                     TTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEVDNNGDHVAAATGNYKVRI
                     DNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAYTYQAEQRGNTVVLQQMEL
                     TDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTI
                     NYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAH
                     FANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVT
                     PYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLA
                     YVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDW
                     SANVGVKYTW"
BASE COUNT         2582 a         2461 c         2350 g         2537 t
ORIGIN      
        1 tggtcattta agccaggaat aaattagctt cccgttcatc atttttgtga tcttgcacac
       61 atctttgaga taacgcactt tcttcaccag tacgttgctc gctttgtcag cagaagattg
      121 caacattctg tcagaaggcc gcgaatcccc cgttttgtcg tggtaatgta tgcactttgt
      181 gttagacggg ataggttttt aagatggaaa aactgcgggt aggaatcgtt tttggtggta
      241 aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa cattgtcgat gccattgata
      301 aaagtcgctt cgatgttgtg ctgctgggca ttgataaaca agggcaatgg cacgtcagcg
      361 atgccagcaa ttatctgcta aatgcagacg atcctgccca tattgcgttg cgcccttcgg
      421 cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca gcttatcgac gcacaaaacg
      481 gtcagccgtt gccgacggtg gatgtcattt tcccgattgt ccacggtacg ctgggcgaag
      541 atggttcctt gcagggaatg ctgcgggtcg ccaatttacc gtttgtaggt tctgatgttc
      601 tggcttcagc ggcctgtatg gataaagatg tcaccaaacg tctgctgcgc gatgccgggc
      661 tgaacattgc gccatttatt accctgacgc gcgctaatcg tcacaacatc agttttgccg
      721 aagtggagtc taaactgggg ttaccgctgt ttgtaaaacc ggctaatcag ggctcttctg
      781 ttggtgtcag caaagtaacc agtgaagaac agtacacaat tgccgtcgat ctggcgttcg
      841 agttcgatca taaagtgatc gttgagcaag ggatcaaagg tcgtgaaatc gaatgcgcag
      901 ttcttggcaa cgataacccg caagcaagca cctgtggcga gatcgtactc accagcgatt
      961 tctatgccta cgacaccaag tacattgacg aagatggcgc gaaagtggta gttccggcag
     1021 ccattgcgcc agaaatcaac gataagatcc gggcgattgc cgttcaggct tatcagacgt
     1081 tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac cccagagaac gaagtggtga
     1141 tcaacgagat caacaccctg cctggcttca ccaacatcag tatgtatccg aagctgtggc
     1201 aagccagcgg tctgggttac accgacctga tcacgcgttt gattgagctg gcgctggagc
     1261 gtcacgctgc ggataacgca ctgaaaacca caatgtaata ttcaaacgcc cggtaatatt
     1321 cttattgggc gttttcagtt tcttcatcgc gacgacgaat aatcaatccc gccagccaga
     1381 aactaatcac ccacgtcacc agaccgacgg cataggtctg ccaacctttt gcttcaaatc
     1441 ccagtaaccc caccacgcca ttaagaatga atataaggcc aatggcgaac gcatagtagt
     1501 gccagtcacg gcggattttt acaggcagat tcatggcagc tccgttaacg caaaacaaca
     1561 tccttgcata ttctgtaaca actgtctgtg gtgtagacaa cactttcgca attgttaata
     1621 aatttaactt aaggttgcta ttttgtgact cacaacgaag attcacgctt cgggaatatt
     1681 cccaacatca atttaccatg attctgatat tgacaatcta aatgacctgt cagacttgcc
     1741 ataagccgct ggtatcacac cagaagagaa gcagatgact tccggaggtt tttatggctg
     1801 atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg
     1861 gcaatattgc ctatgcgttg tttgtgctgt tttgcttttg ggctggggcg caattgctga
     1921 acctgctagt gcatgcgccc ggcgtctatg agcatttaat gcaggtccag gaaacaggtc
     1981 gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt tgggctgatc ccgtttttag
     2041 taggctgcct catttttgca gtagtggcgc tatggctgca ctggcgacat cgccgccagt
     2101 aagatttacg ttgccagatg cggcctaaaa cgccttatct ggcgtgacat tcctgtttga
     2161 tcactgtttc atacaacgcg cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt
     2221 cagattgcgg gtgattttcg ggctttccag ttgaatgcca ggcaacattt cgcggggtag
     2281 tgatttaccg gttttcgctt cggcaagttg ataaactttc ttgtacagcg ccgtctcctc
     2341 aaaagagaag ctgtcccctt tctctaactg acggcgaatt tcgctgtcgc tcatccccag
     2401 ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg ggctctttac tgtcgtaacg
     2461 aattaaatca ccgtccagtg ccagcttcac gccgctggcc ttactgaccg cgttctgaaa
     2521 cgcggcatta cggctggcgt accagccagc gttaaaatca gcaaaacggt atatcggcgc
     2581 gctatagctg gcgggataat tcagtaaatg gtaagtacca aaccacaacc cgccgcgacg
     2641 actgaagact tcctgacgga ctgtaccgtc cattttccac ggatacccct tggtatgctg
     2701 ttcggcaaaa gcaatgctga cctgcatcgg cccacctgtg cgcaccggat tgagcgaacc
     2761 aaacagcgtc tgccccattg gcaccatgct gatcagatcg tcaaaaatcg cgctcaattg
     2821 cttttctgta cgcaccgaat ctaaccgttc gctatagctt ttgccgtttg gcgatttgat
     2881 ttttagcgcg gtatgcacca gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat
     2941 ctcttgccag gcgatcttgc ttaagcccgg aacggccgga tctgcctgat aatttgactc
     3001 ctgctgcgcc accgccagca ccgagcagac gttttccagc gtcggtgcca gcccctgact
     3061 ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat
     3121 cttctgccgc acgacgctcg ccacatctat cgctttttcg ccctttttca acggttgcgg
     3181 agcctgacta ctacaccccg ctaaaaccag aaccgccaac aatgacagcg aagatagagg
     3241 attgacgcgc gacattactt ctccttttta gctcaaggta tgggttactt cctgaatttt
     3301 gtcaccatcc agctcatgtt caaagctgcg cagacgtttg tagatagaca tcaactcaac
     3361 cagtgtggtc catgagttaa tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt
     3421 agtaatctgc gtcatcaggc cgagtgtaat cgtaccggca acaattgacg gaaacagcaa
     3481 gaacaaaccg aaaacgttat cgacctgcaa atagagaatg cgggcgatat tgaaatacat
     3541 atagtgaaaa tagaggcgga aatagttttt ccgtacggcg ctaaaaagct cgcgtaccgt
     3601 aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc agctctttac ggtaggcagc
     3661 ctctacacgc tggtttttaa actccagccc cggcagtttg atccctacca ctgccagtaa
     3721 tccggtcccc atcagcgacc agacgatagc ggcaatcacc agaccatacg gaatgtgccc
     3781 gacaatcggc agctccggca catgtgcgga gagcgttacc agcaccggca ggaaggcgat
     3841 caacgtcatg atagcgttga taaagctgac gcccatattc tccagcgttg aagcaaaacg
     3901 catggtgtct tcctgcacac gctgcgcggc cccttcgata tgacgcagtt gttgccagtt
     3961 cgccatgtaa tattcgttca tcgccgtacg ccagcggaac acgtagtgac tgacaaagaa
     4021 gttgttcagc acactgatca ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc
     4081 gcggtaaaat tgttcgatgg tgactttatg cggcgaactt agcgccgttt gaatcagatc
     4141 atagaacggc gcataccagg cgttgacggc aaccccgact tccaccaaaa accaggtgac
     4201 gaagataatc agcgcagtac cgagtattga ccagtattgc caacggtgcg ggctatagat
     4261 aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa
     4321 atccaacgac cagaaacgcg cggcgctaat cgggatctgc ccggaagcgc cggtgatacg
     4381 cgccacccaa tcacccccac cggcttgcca gaagataacg gcaatcaatg cccaaacaaa
     4441 ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac ttaaacatat gcactcctgc
     4501 taacttctta tcgttttaac aactgctgtg tattaaccgc atgaccgcta caaagcgtca
     4561 gccaaatgaa actgcccatt gtgacccata gcaaggcgct tagttcgcca gcctgaccat
     4621 aagaattgtt tcgacgggtt tcacaaacca aagattgttt acataagcgg attaacacgc
     4681 gcaggatcac aaatgcaggc attggtgagc aggaaaaaga tcgtgtttac gcgccgttac
     4741 gtttttaaca ctattattgc actaattcgc cctttgcaat ccatcaatga gtagtataaa
     4801 tacgctcagt taccttcatt caatctatgg acaccaccgt tgaaacgtag tctgcttttt
     4861 tctgccgtgc tgtatgcggc gtcattgacc tccgtccatg cggctcagcc gatcactgaa
     4921 ccggagtttg cctctgatat tgtcgatcgt tatgccgatc atatttttta cggcagcggt
     4981 gccacgggga tggcgctggt tgttatcgac ggtaaccagc gcgtctttcg cagttatggc
     5041 gaaacgcgac ctggtaataa cgttcgcccg cagctggatt ccgtcatacg tattgcttcc
     5101 ctcaccaagt taatgaccag tgagatgctg gtgaaattgc tcgaccaggg gaccgtgaag
     5161 ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac gcgtaccgac ctacaatggg
     5221 acgcccatta cgctggtgaa tctggcaacc cataccagcg ccctgccccg tgaacagccc
     5281 ggtggcgcgg cacatcgtcc ggtctttgtc tggccaacgc gcgagcaacg ctggaaatac
     5341 ctttccactg ccaaactaaa agccgcgccg ggcagccagg ccgcgtactc caacctcgca
     5401 ttcgatctgc tggcagatgc actggcgaat gcgtccggca agccttatac ccagttgttt
     5461 gaagagcaga ttacccgtcc gttggggatg aaagacacca cctacacccc ctcaccggat
     5521 cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg
     5581 gcaattggca gcggcggcgt ctattccacg ccaggcgata tgatgcgctg gatgcagcag
     5641 tatttgtcat ctgattttta tcagcgcagt aaccaggctg atcgcatgca gacgttgatt
     5701 tatcagcgcg cgcagtttac aaaagtgatt ggcatggatg tccccggcaa agcggatgcg
     5761 cttggtttag gctgggtata tatggccccg aaagagggtc gtccggggat tattcagaag
     5821 acaggtggtg gtggcggttt cattacctat atagcgatga tcccgcagaa gaatattggt
     5881 gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta aaaatatgag cgatggcatc
     5941 aatgacctgg tgaccgagct aagcgggaat aaaccgttgg ttatccccgc atcctgatta
     6001 atactctgaa ggcaaacggt tgatagcgac cagtttgcct ttattcattt cgatatagcc
     6061 gcctttacgc aaagcagcga gaacttccgc aacgacagaa cgcgatatac gtgtacgttg
     6121 atggatataa ttcatcacgc caatacgtga tcgcaattct tcattccagt caatcattga
     6181 taataacgtc gcgcggattt gttcgtagga attatgccca atgagctgaa cgtcgcgtaa
     6241 ttccagaatt ctattttgcc aggctaacca gtaaaaagcg tctcgccaga gttgattttg
     6301 ttcaataaga gtaatggttt gtttggctgg caaatgatat cccgtacagt ttccttctga
     6361 tattaatttg tatggtatat cgtttttcat taaaccatca gccagcccca taatataagg
     6421 agcctgtgta ataccgataa gtacgttttc ttctctacgc aaagagataa cgccctctaa
     6481 aataacgaaa gtatcttcgc tattgacatc actggaaaat ataacttgct tttcattatt
     6541 aaactcgaag cgcgtaccgt atctggacaa acatttatcg agcttaccaa attcctgaag
     6601 aggtttaact acagataaca tttgcgcgtc ctttgcagta atgcccgtca aatccttgac
     6661 gggcattatt tagattaaat tacctatttc ttcggagtga agaatattac caggtatatt
     6721 taacacccac attcgcggac cagtcttgat ctacgtcacc accaccgagg tagttagcat
     6781 cggtataggc gctgaagttc ttggtgaagc tgaactgagt acccagacca acacgtaccg
     6841 cagacccttc agtaccgtta tcgatggaat caccgttcac atcgttatcg ttgttagagt
     6901 cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc ctggtcttcg ctgtaggtga
     6961 aggtataacc tgcatctaca cccagttcat aacgcatgct gtcgtaagac tgaccatcaa
     7021 ctttcatgtc gttgctcagc tggtaatcat caccagactg gaacagacca gaaacgctgc
     7081 cgtaaggagt cacgtaacct gcatcaccca gtttgaagtc gtaaccggct ttcaaaccaa
     7141 agccccaagc gtcggagttg gtgctgccgt caacgtaagt accgttgctc atggttgcag
     7201 acaggtcgtt gttgaagtga gagtagctca agctaccgtc aacaaagacg ttgttcgcga
     7261 agtgagcaga agagtagatg taggcagtct ggctgtcttg atccacctga ccagaacggt
     7321 cattcatatc acctttagcg aagcctgcag ccgcaccgac gatccactta gcgttgttac
     7381 cgtcaatttt ggtatcaaca ccgaccatga tgccgttaac atcctgatca tagttgatgg
     7441 tgccgttgtc gccgttgaag ttaccaccga agtagcttac ccatgcgccg ccgttatcag
     7501 ccagaccatg acgagagttg gtcagacgag taccaacggt gtcttgttcc aggttccaga
     7561 tattggtgtt cgcagacggg atgctcagcg ccatgttagc gtagtcggtc agctccatct
     7621 gttgcagaac aacggtgtta ccacgctgct cagcctgata ggtgtatgca cccaggtcag
     7681 ctttgttagc agcagagaag gtcgcgttgc tgtttttgtc gttgacgtag atcagctcgt
     7741 tgccgttgta gtcagcgata gaaccagcac cagtagcgtt gtcgatacga actttgtagt
     7801 taccggttgc agctgcaaca tggtcaccgt tgttatcaac ttcgttctga ccagcttcga
     7861 tagaagcagt gttgtcgtta tcgccgttac cgttaatggt caggtgacca tcagagttca
     7921 ttgcgattaa gccataaccg tagtttgact tggtagtatc gttggtacgg tcgttggtca
     7981 ggtctgcatc aagtacgaag cggttgcttt cgatgttgaa cacacctgcg tgaatgttac
     8041 cactggtatc agtagtgctg gtcaggttca gagtatcaac ctgcagagca ttcgccgcag
     8101 tgttacctac atccaggata ccgtcgttag tcactttgat gttgttagca tacagagcag
     8161 catcacctac agcccagcca gcagaaactt catcagaaat ggttacggta ctgttgtcga
     8221 cagtcagtga atcagttgca acatgaccat cttcgttgat gttcagcgca gaaagaccag
     8281 tcagagagat agagtcagaa atcagcgttg aatcagcaac attaacagtt gaaccactgt
     8341 tgatgctcag agtatcaatc agagaagttt tggtggtatc ccactgtgaa ccggtgttca
     8401 gggtcacatt gaacagaccg ctctggtaaa cttcgttacc gatgatgtga tcaaattcgc
     8461 tgtattcaga gtcgctgttt tctacaccaa cagttgatga aggccacagg ctgttagctg
     8521 cgatgtcgat cagagttgca gtggcttcag tattgattcc atatgtatag gagtcattta
     8581 caccatcgtc atcagtatca acaccaatct gatgcgcaga cattgccgca ccgacccact
     8641 tgctgccatt gttcagggtc agatccaggc gatcagtacc atcccaaccg ttagtatcta
     8701 catcaccgtc agcatcacga tagctatcac gaccttgtgg gaagaagttc tcatcaaaat
     8761 tacttgagaa gaatacatcg cccatcagtg tggaattgtc gaatgttgca gtggtttgca
     8821 tggcgttatc agcatatgga tgtgcaataa cagctaatgc aacatcatca gcattccacc
     8881 catcaccatt gtaatcacta gcattaccag tattaccaaa ccaaccagta gtaccttcat
     8941 cagtccatga accagaggtc acggtagaat ctttaacagt gatggtgtta ttaaatactt
     9001 cgctgctgtt tacaccagtg ctgacagtgt tgtcgtcgat atcttcccac tcataaccct
     9061 gagtcagcgt aatacctgct acatgagagt tattagagat ggacagatca acttcctgat
     9121 ccagagtgat ggcagtacca atgttaaata catttacaac atgagtgtca gcggcattat
     9181 tgtaggtgcc gttatatgtg tagtgttcgt agttgtcatc gatcgttgaa ttatcaacgg
     9241 tcagcgccag acgatcatgg tagtagtcat ccgcacgatc agcacaatca gttgtcatgc
     9301 actcggaata aatcatgccg tgaatagtgc tgtttttgat tgtcaggcta ttggcattgg
     9361 tattagtaga aataccatcg tccagatagt aggtagaaat cacgccgtta acggtagcgt
     9421 tattaatgac cggatagata tcgccgttgt aataactatc agcggcatag ttattccagc
     9481 ctacataacc atcatagtaa acgtcatcag cataggtggc gtcgttgtag tgatggaaag
     9541 tattgtaagt ttgaccagaa atatcgttgg tcgctgcatt agcctgagag gtgatagcca
     9601 gagtgcaagc caatgctaat tgtgatacta caagtttctt tttccaggag tgcatttgtc
     9661 atccctcctc agggacgtaa taagttgatc catcaattat caatgcatag aaaacatatt
     9721 atgtttcgcg tttacgatta tgcggcgtta tagaggaatg gttcaattat tctttcccct
     9781 aagtccgatt gcggacatat ataaagcacg tgaacacaca caaagaaaga aataacaaaa
     9841 acacaatatt tcaatacgtt aatgaaagcg ttaattttaa aacatagacc atgaaaatat
     9901 aaaacaacaa aaacagacaa ataatgtatc
//
LOCUS       DAZGJT010000138         9843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZGJT010000138 DAZGJT010000000
VERSION     DAZGJT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.140"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..761
                     /locus_tag="ACHKFK_004225"
     CDS             <1..761
                     /locus_tag="ACHKFK_004225"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFO9054444.1"
                     /translation="TLFKSWFVDFDPVIDNALDAGNPIPEALQARAELRQKVRNSTDF
                     KPLPAEIRSLFPSEFEETELGWVPGGWKTNRLENILELAYGKALKKTERIEGDYPVYG
                     SGGVDGSHNEFLVKGPGIIVGRKGTVGSLYWENKDFYPIDTVFYVKPKKYFSLVYCYQ
                     LLKTLGLENMNTDAAVPGLNRNNAYRLDVITPTQTIIAQYTNIVQTFRYKMDSNNNEI
                     DNLTNLRDTLLPKLISGELSLDDIPDLNTDTEAA"
     gene            856..4119
                     /locus_tag="ACHKFK_004226"
     CDS             856..4119
                     /locus_tag="ACHKFK_004226"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HFO9054445.1"
                     /translation="MSLSFSEAKLEQAIIELLQDQGYQHLIGDNVPRSSLDQVIIEDD
                     LRHYLAARYQPDGITEEEIQRLIKQFTTLPASDLYESNKTFCRWLANGFLFKRDDRQQ
                     KDLYIELLDTRHLPAALRQIFDAEDVPLQQVAELPPSYINPPLNRIKIVNQLKISGKD
                     NQTRIPDGILYINGLPLVVFEFKSAVREQEASIGDAWKQLCKRYRRDIPQLFIYNALC
                     IISDGVNNRMGNLFAPYEYFYSWRKVTGNENREQDGIPSLHSMIQGLFHPVRLLDVIK
                     NFICFPDKAKHEVKICCRYPQYYAARKLYYSIKQARKPFGSGKGGTYFGATGCGKSYT
                     MQFLTRLLMKSVEFASPTIVLITDRTDLDDQLSAQMCNAKNYIGDDTIVPVTSREDLR
                     NQLAGRNSGGVFLTTIHKFTEDTELLSERSNIICISDEAHRSQVNLDQKVIVDKESGK
                     VRKTYGFAKYLHDSLPNATYVGFTGTPIDATLDVFGEVIDSYTMTESVQDEITVRIVY
                     EGRAAKVILDSSKLEEVEKYYEECANAGTNEWQIDESKKATATMNAVLGDEDRLKALA
                     EDFAKHYEKRVAEGSTVIGKAMFVCASREIAWDFYRQLKAIRPAWFEVKQAPDGVVLT
                     EQEQKELPPSEMVKMVMTRGKDDDEALYDLLGTKEYRKELDKQFKNAKSNFKIAIVVD
                     MWLTGFDVPELDTIYIDKPLQKHNLIQTISRVNRKLEGKSKGLVVDYIGIKSQMNQAL
                     AMYSRIDATNFEDIQQSVIEVKNHLDLLGQVFYEFDSRDYFSGEPQAQLSCLNRAAEF
                     VLRTQKVERRFMGLVKRMKAAYDVCCGSEALSQTERDYIHYYLAVRSIVFKLTKGDAP
                     DVTQMNARVREMISEALKADGVEEIYFLGDKKAESIDIFDEDYLARINKIKLPATKIQ
                     LLQKLLEKAISDFKKVNQLQGINFTRRFQAIIDRYNERREDDVLNGEEFDTFSQEMTD
                     IIYDIKTEMGTWADLGIDIEEKAFFDILAHMRDKYQFTYDDEKMLSLAKEMKSVVDNT
                     SKYPDWSKRDDIKAKLKVELILLLHKHKFPPVANDDVYMGVLAQAENFKEHHMSALN"
     gene            4334..4672
                     /gene="symE"
                     /locus_tag="ACHKFK_004227"
     CDS             4334..4672
                     /gene="symE"
                     /locus_tag="ACHKFK_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HFO9054446.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLETAGFATGTAVDVKVMEGCIVLTAQPPAAEESKLMQSLRQVCKLSARKQKQVQEF
                     IGVISSKTPR"
     gene            complement(4715..4879)
                     /locus_tag="ACHKFK_004228"
     CDS             complement(4715..4879)
                     /locus_tag="ACHKFK_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO9054447.1"
                     /translation="MEFHENKAKAPFTGIVQLWQAVRTWWLKKQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            5056..5364
                     /locus_tag="ACHKFK_004229"
                     /pseudo
     CDS             5056..5364
                     /locus_tag="ACHKFK_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5362..6282)
                     /locus_tag="ACHKFK_004230"
     CDS             complement(5362..6282)
                     /locus_tag="ACHKFK_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO9054448.1"
                     /translation="MTNFTTSTPHDALFKSFLTHSDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRQLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYTAAFPLVDI
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFNEFISEITRRLPQQRERIMTIAERIHNDGYIKGEQRILRLFLQNGADPEW
                     IQKITGLSAEQMQALEQPLPDREHDSWLEY"
     gene            6467..7747
                     /gene="yjiN"
                     /locus_tag="ACHKFK_004231"
     CDS             6467..7747
                     /gene="yjiN"
                     /locus_tag="ACHKFK_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO9054449.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPVISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTRNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIEKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNL"
     gene            7863..9014
                     /gene="yjiM"
                     /locus_tag="ACHKFK_004232"
     CDS             7863..9014
                     /gene="yjiM"
                     /locus_tag="ACHKFK_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO9054450.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPHPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVSQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9024..9791
                     /gene="yjiL"
                     /locus_tag="ACHKFK_004233"
     CDS             9024..9791
                     /gene="yjiL"
                     /locus_tag="ACHKFK_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298028.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO9054451.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLEPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVIPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQRF
                     THMLESHLGMPVQTHPDAQFAGAIGAAVIGQRQRKRA"
BASE COUNT         2608 a         2365 c         2417 g         2453 t
ORIGIN      
        1 aaaccctgtt caaatcatgg tttgtggatt ttgatccggt gattgataac gcgctggatg
       61 caggaaatcc aatcccggaa gccctgcaag ctcgtgccga attgcgtcaa aaagtacgta
      121 atagtacaga ttttaaaccg cttccggcgg aaattcgctc gcttttccca agtgaatttg
      181 aagagacgga gttaggttgg gtgccaggag gctggaaaac aaaccgactg gaaaatattt
      241 tagagctggc ttatgggaaa gcactcaaaa aaacagaaag aatcgaagga gattatcccg
      301 tttatggttc tggtggggtg gatggttccc ataatgagtt tcttgttaaa ggacctggta
      361 ttattgttgg aaggaaagga actgttggtt cattgtattg ggagaacaaa gatttttacc
      421 caattgatac agtattttat gtaaaaccta aaaagtactt ctcgcttgtt tactgctatc
      481 agctcttgaa aacactagga ttagaaaata tgaatactga tgcggcagtc cctgggttga
      541 acagaaacaa tgcttatcgt cttgacgtca taacaccaac acaaacaatc attgcccaat
      601 atacaaatat agtacaaacg ttcaggtata aaatggattc aaataataat gagattgaca
      661 atctaaccaa cctccgcgac accctgctcc cgaaactcat ctccggcgag ctatccctgg
      721 acgatattcc ggatctcaac accgatacag aagccgcata acgcattttg cccctgtaaa
      781 atcaggggct ttctggtaag gttttctact gatacgggaa tgcttaccag aaattagcca
      841 gggttggagc gcgatatgag tctctctttc agtgaagcaa aattagaaca agcgatcatt
      901 gaactgttac aggatcaggg atatcaacac ctgatcggcg ataatgtccc acgttcaagt
      961 ctcgatcagg tcattatcga agacgatctc cgtcattatt tagcggcacg ctaccagcct
     1021 gatggcatta ctgaagaaga gattcagcga ctgatcaaac agttcaccac gcttccggcc
     1081 tccgatcttt atgaaagcaa caaaacattt tgtcgctggc tggcaaatgg ttttctgttc
     1141 aaacgcgacg atcggcaaca aaaagatctc tatattgaat tgctcgacac ccggcatcta
     1201 cctgccgcgc tgcgccagat atttgacgcc gaagatgtcc cgttgcaaca ggtggcagaa
     1261 ctcccgccct cctatattaa tccgccgctt aaccggatta agattgttaa ccagcttaaa
     1321 atctccggca aagataatca gactcgtatt cctgacggca ttctctatat caacggtctg
     1381 ccactggttg tctttgaatt taaaagtgcg gtacgcgagc aggaagccag tattggcgat
     1441 gcctggaagc agctctgtaa acgctaccgc cgggatattc cacaactgtt tatctacaac
     1501 gcgctctgca ttattagcga tggcgtaaat aaccggatgg gcaacctgtt tgcgccctat
     1561 gaatactttt actcatggcg caaagtcacc ggtaatgaaa accgtgaaca ggatggaatt
     1621 ccatcactgc actcaatgat tcaggggctg tttcatccgg tacgtctgct ggatgtgatt
     1681 aaaaacttta tctgcttccc ggataaagcc aaacatgaag taaaaatttg ctgccgatat
     1741 ccgcagtact atgccgcccg caaactctat tacagcatca agcaagcacg taaacctttc
     1801 ggtagcggta aaggcggcac ctactttggc gcaacgggct gtggcaaaag ttacaccatg
     1861 caatttttaa cgcgtctttt gatgaagagc gtggagtttg ccagcccgac cattgttttg
     1921 atcaccgacc gtaccgatct ggacgatcag ctttctgcgc aaatgtgcaa cgccaaaaat
     1981 tacattggcg atgacaccat cgttcccgtt accagccgtg aagatttgcg taatcaactg
     2041 gcgggacgca atagtggcgg tgtattcctg acaacgatcc ataaattcac cgaagacacc
     2101 gaactccttt ctgaacgcag caatatcatt tgcatctcgg acgaagcaca tcgcagccag
     2161 gttaacctcg accagaaagt catcgtcgat aaagaaagtg gaaaagtgcg caaaacgtat
     2221 ggctttgcga aatacctgca cgattcactg ccaaacgcca cctatgttgg ctttaccggc
     2281 acgccgattg acgcgacgct cgatgtcttc ggtgaggtga tcgacagtta cacaatgacc
     2341 gaatccgttc aggatgagat cactgtacgc atcgtatacg aaggtcgtgc ggctaaagtg
     2401 atcctggact ccagcaaact ggaagaagtc gaaaagtatt acgaagagtg cgcaaacgca
     2461 ggcaccaatg agtggcaaat cgacgaaagc aagaaagcca cggctaccat gaatgcggtt
     2521 ctgggtgatg aagatcggtt aaaagccctc gcggaagatt ttgctaaaca ttatgaaaaa
     2581 cgcgtagccg aaggttccac cgtaataggc aaagccatgt ttgtttgtgc cagccgtgaa
     2641 attgcctggg atttctaccg ccagcttaaa gctattcgcc ctgcctggtt tgaagtgaag
     2701 caagcacctg atggcgtcgt cctgacggaa caggaacaaa aagagttacc gccttctgaa
     2761 atggtgaaaa tggtcatgac gcgcggtaaa gatgacgacg aggcgcttta tgatttactg
     2821 ggcacgaaag aatatcgtaa agagctggat aagcagttca aaaacgctaa atcgaatttc
     2881 aaaattgcca ttgtcgttga tatgtggctg accggttttg atgttcctga actggatacc
     2941 atctatattg ataagccctt acaaaaacat aaccttatcc agactatttc tcgcgttaac
     3001 cgtaagctgg aaggcaaaag caaagggtta gtggtggact acatcggcat taaaagtcag
     3061 atgaaccagg cactggcaat gtattcccgc attgatgcca ccaactttga agatattcag
     3121 caatcggtaa tagaagttaa aaaccatctc gatttgttgg ggcaagtctt ttatgaattt
     3181 gacagtcggg attattttag tggtgagcca caagcgcaat tatcctgcct caaccgcgcg
     3241 gcggaatttg ttctgcgcac tcagaaagtt gaacgtcgtt ttatgggact ggttaaacgc
     3301 atgaaagccg cctacgacgt ctgctgcggc agtgaagcac tatcacagac agaacgtgac
     3361 tatattcact attatcttgc tgttcgttca attgttttca aactgacgaa aggtgacgca
     3421 ccggatgtta cccagatgaa tgcacgcgtt cgtgaaatga tttcagaagc gttaaaagct
     3481 gatggcgtag aagaaattta ttttcttggc gacaaaaaag cggaatccat cgatattttt
     3541 gacgaagatt atctggcgcg aattaacaag atcaaacttc cggcaacgaa gatccagtta
     3601 ttacaaaaat tactggaaaa agcgatcagc gacttcaaga aagtgaacca gttgcaaggg
     3661 attaacttca cccgccgctt ccaggcgatt atagatcgtt ataatgagcg gcgagaagat
     3721 gatgtactca acggtgaaga attcgataca ttcagtcagg aaatgaccga tattatctat
     3781 gatattaaaa ccgaaatggg cacctgggcc gatttaggta ttgatattga agaaaaagcc
     3841 tttttcgaca ttcttgctca tatgcgcgat aaatatcagt tcacctatga cgatgaaaaa
     3901 atgctgtcac tggcaaaaga gatgaaaagc gtggttgaca acacatcgaa atatcctgac
     3961 tggagtaaac gcgatgatat taaagcgaaa ctgaaagttg aacttattct gcttctacac
     4021 aagcataagt tcccgccagt agcgaatgat gatgtttata tgggtgtgct ggcacaggca
     4081 gagaatttta aagaacatca tatgagtgct ttaaattaat tatgaaggcc ggatacactt
     4141 tgttatccgg ccattctgaa ttaacgttaa tttctcttcc agatctttcc agtaacgctt
     4201 ttattatttt ctatttttag ctggactttc cctgtattta ctgatgatat gtccaggtat
     4261 ttagcgcggt acggatgtgc gccaacacac ccgcaccgct catcacaatc actattttca
     4321 aggaatagca gttatgactg acacgcattc tattgcacaa ccgttcgaag cagaagtctc
     4381 cccggcaaat aaccgtcaat taaccgtgag ttatgcgagc cgctacccgg attacagccg
     4441 tattcccgcc atcactctga aaggtcagtg gctggaaacc gccggttttg ccactggcac
     4501 ggcggtagat gtcaaagtga tggaaggctg tattgtcctc accgcccaac cacccgccgc
     4561 cgaagagagc aaactgatgc agtcactgcg ccaggtgtgc aagctgtcgg cgcgtaaaca
     4621 aaagcaggtg caggagttta ttggggtgat tagtagcaaa acgccacgct aagattctct
     4681 ccccttcccc acacgggaag gggaattttc ctggtcactc cacatcctcc ctgcgtaacc
     4741 caatatccct caaccgctca tcactcatct gctgtaacac gcgccgggtc tgctttttca
     4801 gccaccaggt cctcaccgcc tgccagagtt gtacgatgcc agtaaacggc gctttggctt
     4861 tgttttcgtg aaattccatc tgctgcctcc tcatctttca gaggcttcat catccgcctt
     4921 ttggcagttc acaatacaga ttcaaatatt acttttattt aacatacaga cagattaaaa
     4981 cagcatctgc atccggtttt taagcctcat ctgtaccggt ttttctctct gtatggtcca
     5041 aagaaggaat acagcatgac gcgttatcaa catctggcga ctctgcttgc cgagcggatt
     5101 gagcaagggc tgtatcgtca cggggagaaa ttgccgtcgg tgcgcagctt aagtcaggag
     5161 cacggcgtca gcatcagtac cgtgcagcag gcgtatcaga tactggagac gatgaagctc
     5221 atcactccgc agccgcgttc gggttatttt gtcgcgcaac gtaaagccca gccgccagta
     5281 ccgtcgatga cgcgtccggt ggaaattacc cagtgggatc aggtgctgga tatgctggag
     5341 gcacatagcg atagttccat attagtactc gagccatgaa tcgtgctcac gatcaggcaa
     5401 gggctgctct aatgcctgca tttgctctgc cgaaagtccg gtaatctttt gtatccattc
     5461 aggatctgcc ccattctgca aaaacaatcg aagaatgcgc tgttcccctt ttatatatcc
     5521 atcattatga attcgctctg caatcgtcat tattcgctcc ctttgttgtg gcagtcgacg
     5581 ggtaatttca ctgataaact cattaaaacg cgcttcatca ccagtcagta aaatgtaatt
     5641 taacagcgca gttatctggc tgtcattagc acactctgta accagtaata ctaccagttg
     5701 gtcaataagc cccatcagat cgcgctggcg aatatgcttt tggatcaact ccaacagggc
     5761 gactctgcga tgctgcacaa tctcgtcgtc tggcacgaca gtgatatcca ccagcgggaa
     5821 cgcggcggta taaagcttcc gtgcggtagt cgggtcggca aattcgtcca gccagcacaa
     5881 agaccaggga taagggctac ggctaccgtg ataaaacagc atcgggatga ccagcggtag
     5941 ctgccggcgt ttatcatgtt cgatatggcg ctgcatcacc gccatggaat atcgcatcag
     6001 acgaaaggcc atatgaatat cctcacggct ctgatgttca atcactacat aaatatagcc
     6061 atcgccttca cgggtcttta ccgaccacag aatatcggaa tgtagcgccc gcaatttttc
     6121 atcgacaaag ctggcggatt ccagttttaa gctatcgaga tcgcacagtt cacgtaaatc
     6181 tttgggtaag tggatctcca taaaatcccg cgcggtgtca gagtgtgtga gaaaggattt
     6241 aaataatgcg tcatgcggcg tgctggtcgt aaagtttgtc atggcgatcc gtcacctgta
     6301 gataagatgg cccaacttta gcgactaaca aagccattca cacctggcta tttcctattc
     6361 cccgaggcgg tttccaggct atttttattc ttctgcaaac gatacatttt tatggaagat
     6421 atctcgcttt ttgcgttttc ttaccgtcag gctctattct tacgatatga ataaactcat
     6481 tgaactcaga cgcgccaaaa tgttggcgct ctctttactg cttatcgccg ctgctacctt
     6541 tgtcgttacg ctgtttttgc cgcccaattt ttgggtgagc ggtgtgaagg cgattgctga
     6601 agcggcgatg gtcggcgcgc tggcggactg gtttgcggtg gtggcgctgt ttcgccgcgt
     6661 gccgattccg gtcatttctc gtcatacggc gattatcccg cgtaataaag accggattgg
     6721 cgaaaatctc ggccagttcg tgcaggaaaa atttctcgat acccaatcgc tggtggcatt
     6781 gattcgacgt cacgaaccgg cgttgttgat tggcaactgg tttagtcagc cggagaacgc
     6841 ccgccgcgtt ggtcagcatc tgttacagat catgagcggt tttctcgaac tgaccgatga
     6901 tgcgcgtatt cagcgcctgc ttaagcgtgc ggtccaccgg gcgatcgata aggtcgatct
     6961 ttccggcacc agtgcgttga tgctggagag tatgaccaga aacgatcgtc atcaggtgct
     7021 gctcgatacg ctgatcgcac aactgatcgc cctgcttcag cgcgataaat cacgcaagtt
     7081 tattgcccag caaattgttc gctggctgga gagcgagcat ccactgaaag ccaaaatttt
     7141 gcccactgaa tggctgggcg aacatagcgc ggagttggtt tctgacgcgg tgaattcttt
     7201 gcttgatgat atcagccgcg atcgtgcgca tcagatccgc catgcgtttg atcgcgctac
     7261 cttcgccctg atcgagaaac tgaaaaacga tccggaaatg gcagcgcgag ccgatgccgt
     7321 aaaaagctat ctgaaagaag atgaagcttt taaccgctat ctcagtgaat tgtgggggga
     7381 tttacgggaa tggctgaaag cggatatcaa cagtgaagat tctcgtgtga aagaacgtat
     7441 cgcgcgggcg gggcaatggt ttggcgaaac gttaattgcc gatgatgcct tgcgggcgtc
     7501 gttaaatggt catctcgaac aagccgcaca ccgcgttgcg cctgagtttt ccgcattcct
     7561 gactcgccat atcagcgaca cggtaaaaag ctgggacgca cgggatatgt cgcggcaaat
     7621 cgagttaaat atcggcaaag atctccagtt tatccgtgtc aacggtacgc tggttggcgg
     7681 ttgtattgga cttattttgt atttgctgtc gcagctcccg gccttgttcc ccctcggcaa
     7741 tttatagaaa tccatgaaaa actaagtggc taaccgcgag agagatcaaa taaaccacac
     7801 taaaagaatg gtaataatgt ggcctctttt cattatctcc cgtggtacgg ggaaggaaaa
     7861 tcatgtcact tgtcaccgat ctacccgcca ttttcgatca gttctctgaa gctcgccaga
     7921 aaggctttct caccgtcatg gatctcaagg agcgcggcat tccgctggtt ggcacctact
     7981 gcacctttat gccgcaagag atcccgatgg cagccggtgc ggttgtggtt tcgctatgtt
     8041 ccacctctga tgaaaccatt gaagaagccg agaaagatct gccgcgcaac ctctgcccgc
     8101 tgattaaaag cagctacggc ttcggcaaaa ccgataaatg cccctacttc tacttttctg
     8161 atctggtggt cggtgaaacc acctgcgacg gcaaaaagaa aatgtatgaa tacatggcgg
     8221 agtttaagcc cgttcatgtg atgcaactgc caaacagcgt taaggacgat gcctcgcgtg
     8281 cattatggaa agccgagatg ctgcgcttgc aaaaaacggt agaagaacgt tttgggcacg
     8341 agattagcga agatgctctg cgcgatgcca ttgcgctgaa aaaccgcgaa cgtcgcgcac
     8401 tggccaactt ttatcatttg gggcagttaa atcctccggc gcttagcggc agcgacattc
     8461 tgaaagtagt ctacggcgca accttccggt tcgataaaga ggcgttgatc aatgaactgg
     8521 atgcaatgac cgcccgcgtt cgtcagcagt gggaagaagg ccagcgactg gacccgcacc
     8581 cacgtatttt aatcaccggc tgcccaattg gcggcgcagc agagaaagtg gtgcgcgcga
     8641 ttgaagagaa tggcggctgg gttgtcggtt atgaaaactg caccggggcg aaagccaccg
     8701 agcaatgcgt ggcggaaacg ggtgatgtgt acgacgcgct ggcggataaa tatctggcga
     8761 ttggctgctc ctgtgtttcg ccgaacgatc agcgcctgca aatgctcagc cagatggttg
     8821 aagaatatca ggtcgatggc gtagttgatg tgattttgca ggcgtgccat acctacgcgg
     8881 tggaatcgct ggcgattaaa cgtcatgtga gccagcagca caacattcct tatatcgcta
     8941 ttgaaacaga ctactccacc tcggatgtcg ggcagctcag cacccgtgtc gcggccttta
     9001 ttgagatgct gtaaggagtg gcagtgacat attcgattgg cattgattcc ggctcaaccg
     9061 ccaccaaagg gatcttactg gcagacggcg tgattacgcg ccgtttcctc gttccaacac
     9121 cctttcgccc ggcaacagca attactgaag cctgggaaac tctgcgcgaa gggttagaga
     9181 caacaccgtt tctgacgctc accggctacg gacggcaact ggtggatttt gccgataaac
     9241 aagtgacgga aatctcctgt cacgggctgg gcgcacggtt tcttgagcca gcaacgcgcg
     9301 cggtaatcga catcggtggt caggacagca aagtgattca gcttgatgat gacggtaacc
     9361 tgtgcgattt cctgatgaat gacaaatgcg cggcgggcac cgggcgtttc ctggaggtga
     9421 tctcgcgcac gcttggcacc agcgtcgagc aactcgacag cattaccgaa aatgtcatac
     9481 cgcacgccat cacgagtatg tgcacagtgt ttgctgaatc agaagtgatc agcctgcgct
     9541 ctgcgggcgt agcgccagaa gcgattctcg ccggagtgat taacgcgatg gcgcggcgga
     9601 gcgccaattt cattgctcgt ctctcctgtg aagcgccgat tctgtttact ggtggcgtta
     9661 gccactgtca aaggtttacc cacatgctgg aaagccatct gggaatgccg gtacaaactc
     9721 accctgatgc gcaattcgct ggtgcaattg gcgcggcggt gatcggtcag cggcagagaa
     9781 aacgcgcatg aaagagtatc tgtttttatt tcactccacg ggtaatgact ccaacttatt
     9841 gat
//
LOCUS       DAZGJT010000139         9357 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZGJT010000139 DAZGJT010000000
VERSION     DAZGJT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.142"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..146)
                     /locus_tag="ACHKFK_004234"
     CDS             complement(<1..146)
                     /locus_tag="ACHKFK_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9054452.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTAS"
     gene            complement(233..301)
                     /gene="ykiE"
                     /locus_tag="ACHKFK_004235"
     CDS             complement(233..301)
                     /gene="ykiE"
                     /locus_tag="ACHKFK_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO9054453.1"
                     /translation="MGWSICFWHVSVRMTHDVVCYY"
     gene            438..959
                     /gene="fecI"
                     /locus_tag="ACHKFK_004236"
     CDS             438..959
                     /gene="fecI"
                     /locus_tag="ACHKFK_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO9054454.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            956..1909
                     /gene="fecR"
                     /locus_tag="ACHKFK_004237"
     CDS             956..1909
                     /gene="fecR"
                     /locus_tag="ACHKFK_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO9054455.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            1996..4320
                     /gene="fecA"
                     /locus_tag="ACHKFK_004238"
     CDS             1996..4320
                     /gene="fecA"
                     /locus_tag="ACHKFK_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO9054456.1"
                     /translation="MTPLRVFRKTTPLVNTIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLSPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYLNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEIGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4365..5267
                     /gene="fecB"
                     /locus_tag="ACHKFK_004239"
     CDS             4365..5267
                     /gene="fecB"
                     /locus_tag="ACHKFK_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO9054457.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5264..6262
                     /gene="fecC"
                     /locus_tag="ACHKFK_004240"
     CDS             5264..6262
                     /gene="fecC"
                     /locus_tag="ACHKFK_004240"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO9054458.1"
                     /translation="MTAIKHPVLLWGLPIAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6259..7215
                     /gene="fecD"
                     /locus_tag="ACHKFK_004241"
     CDS             6259..7215
                     /gene="fecD"
                     /locus_tag="ACHKFK_004241"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO9054459.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7216..7983
                     /gene="fecE"
                     /locus_tag="ACHKFK_004242"
     CDS             7216..7983
                     /gene="fecE"
                     /locus_tag="ACHKFK_004242"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO9054460.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMK"
     gene            complement(8542..8799)
                     /locus_tag="ACHKFK_004243"
     CDS             complement(8542..8799)
                     /locus_tag="ACHKFK_004243"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054461.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(8882..>9357)
                     /locus_tag="ACHKFK_004244"
     CDS             complement(8882..>9357)
                     /locus_tag="ACHKFK_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9054462.1"
                     /translation="TGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETR
                     GKPVGVMFHSDQGSHYTSRQFRQLLWRYRIRQSMSRRGNCWDNSPMERFFRSLKNEWV
                     PATGYVSFSDAAHAITDYIVGYYSALRPHEYNGGLPPNESENRYWKNSNAEASFS"
BASE COUNT         2043 a         2665 c         2583 g         2066 t
ORIGIN      
        1 tgagaggcgg tgtaagtgaa ctgtagttgc catgttttac ggcaatgaga gcagagatag
       61 cgctgatgtc cggcagtgct tttgccgtta cgcaccacgc cttcagtagc ggagcaggaa
      121 ggacatctga tggaaatgga agccacgcaa gcaccttaaa atcaccatca tacactaaat
      181 cagtaagttg acagcattac cgaaatcccc atcctttagg gcagtgagga tgtcaataat
      241 agcagaccac atcatgagtc attctgaccg acacatgcca gaagcagatg ctccatccca
      301 ttttatacct accttataac acttagaaaa acaacatgtt aaaaatgtct attggaaaca
      361 attttatttc caattgtaat gataaccatt ctcatattaa tatggctacg tgataattaa
      421 cttttgatgc actccgcatg tctgaccgcg ccactaccac agcttcctta acgttcgagt
      481 cgctttatgg cacacatcac ggctggttga aaagctggct gacgcgcaaa ctccagtctg
      541 cttttgatgc agatgacatt gcccaggaca cttttttgcg ggtaatggtc agcgaaacgc
      601 tctcgacgat ccgcgatcct cgctccttcc tgtgcactat cgccaaacgc gtgatggtgg
      661 acctgtttcg ccgaaacgcg ctggaaaaag cgtatctgga gatgctggcg cttatgccgg
      721 aggggggagc gccttcacct gaggaacgcg aaagccaact cgagacccta caactcctcg
      781 acagcatgct ggacgggcta aacggcaaaa cacgtgaagc gtttctgctt tcgcaactgg
      841 atggtctgac atacagcgag attgcgcaca aactcggtgt ttccatcagc tccgtgaaaa
      901 aatacgtggc gaaagccgtc gagcactgcc tgctgttccg tctggagtat gggttatgaa
      961 tcctttgtta accgattccc gccgtcaggc gctgcgttca gcttcccact ggtatgccgt
     1021 tctaagcggc gagcgcgtca gcccacaaca ggaagcgcgc tggcaacagt ggtatgaaca
     1081 ggatcaggat aaccagtggg cctggcagca ggttgaaaac ctgcgcaacc agcttggcgg
     1141 tgtgcctggc gacgttgcca gccgggcgtt gcacgatacc cgcctcaccc gccgtcacgt
     1201 gatgaaagga ttactgctgt tgctcggcgc tggcggaggc tggcagctct ggcagtcgga
     1261 aaccggcgaa ggtctgcggg cagattaccg caccgccaaa ggcacagtca gccgtcagca
     1321 actggaagat ggctccctgc tcacgctgaa tacccaaagc gcggcggatg tgcgttttga
     1381 tgcgcatcag cgcaccgtcc ggctctggta cggtgaaatc gccattacta ccgcgaaaga
     1441 tgccctgcaa cgccccttcc gtgtcctgac ccgtcagggc cagctcactg ctttagggac
     1501 agaatttacc gtccgccagc aggataattt cacgcagctt gacgtgcagc agcacgctgt
     1561 ggaagtgctt ctcgccagtg cccccgcgca aaaacgcatc gtgaacgctg gtgaaagcct
     1621 gcagttcagc gcctctgagt ttggcgcagt gaaaccgctg gatgacgaga gtacaagctg
     1681 gacgaaggac atcctgagct tcagcgataa accgctgggt gaggtgatag ccacgctaac
     1741 ccgttaccgc aacggcgtgc tgcgctgcga tcccgccgtt gccgggctgc gcctgagcgg
     1801 gacgttcccg ctgaaaaata ccgatgcgat cctgaacgtt atcgcgcaaa cgcttcccgt
     1861 taaaattcag tctattacgc ggtactggat aaacatttca ccactgtaag gaaaataatt
     1921 cttatttcga ttgtcctttt tacccttctc gttcgactca tagctgaaca caacaaaaat
     1981 gatgatgggg aaggtatgac gccgttacgc gtttttcgta aaacaacacc tttggttaac
     2041 accattcgcc tgagcctgct gccgctggcc ggtctctcgt tttccgcttt tgctgcacag
     2101 gttaatatcg caccgggatc gctcgataaa gcgctcaatc agtatgccgc acacagcgga
     2161 tttaccctct cggttgacgc cagcctgacg cgcggcaagc agagcaacgg cctgcacggc
     2221 gattacgacg tcgagagcgg cctgcaacaa ctgctggacg gcagcggact gcaggtaaaa
     2281 ccgctgggaa ataacagctg gacgctggag cccgcgcccg caccaaaaga agatgccctg
     2341 accgtggtcg gcgactggct gggtgatgcg cgtgaaaacg acgtatttga acatgctggc
     2401 gcgcgtgacg tgatccgccg tgaggatttc gccaaaaccg gcgcaaccac catgcgtgag
     2461 gtacttaacc gcatccctgg cgtcagcgcg ccggaaaaca acggcaccgg cagccacgac
     2521 ctggcgatga actttggcat ccggggcctg agcccgcgcc tcgccagccg ctcgaccgtc
     2581 ctgatggacg gcatccccgt ccccttcgcc ccttacggtc agccgcagct ttcactggct
     2641 cccgtttcgc tcggcaacat ggatgccatt gacgtggtac gcggtggtgg tgcggtgcgt
     2701 tacggaccgc agagcgtggg cggcgtggtg aactttgtta cccgtgccat tccgcaggac
     2761 tttggtatcg aggcgggcgt ggaaggtcag ctcagcccaa cctcttcaca aaacaacccg
     2821 aaagagacgc acaacctgat ggtgggcggc acagcggaca acggttttgg caccgcgctg
     2881 ctctactccg gcacgcgcgg cagtgactgg cgcgagcaca gcgccacccg catcgacgac
     2941 ctgatgctga aaagcaaata tgcgccggat gaggtgcaca ccttcaacag cctgctgcaa
     3001 tattacgacg gtgaagccga catgcccggt ggcctgtctc gcgcggatta cgacgccgat
     3061 cgctggcaat ccacccgccc gtatgaccgc ttctggggtc gtcgcaagct ggcgagcctg
     3121 ggctaccagt tccagccaga cagccagcat aaattcaaca ttcaggggtt ctacacccaa
     3181 accctgcgca gcggctacct ggagcaaggc aaacgcatca ccctctcgcc gcgtaactac
     3241 tgggtgcgcg gtattgagcc acgctacagc cagatcttta tgatcggccc ttccgcgcac
     3301 gaagtgggcg tgggctatcg ctatttgaat gaatcaacgc atgaaatgcg ttactacacc
     3361 gccaccagca gcgggcagtt gccgtccggc tcaagccctt acgaccgcga tacgcgttcc
     3421 ggcaccgagg cgcacgcctg gtatctggat gacaaaatcg acatcggcaa ctggaccatc
     3481 acgccgggta tgcgtttcga acatatcgag tcataccaga acaacgccat cacaggcacg
     3541 cacgaagaag tgagctataa cgcaccgctt ccggcgttga acgtgctcta tcacctgact
     3601 gacagctgga atctttatgc aaacactgaa ggctcgttcg gcaccgtaca gtacagccag
     3661 attggcaagg ctgtgcaaag cggcaatgtg gaaccggaaa aagcgcgaac ctgggaactc
     3721 ggtacccgct acgacgacgg cgcgctgacg gcggaaatag ggctgttcct gattaacttt
     3781 aacaatcagt acgactccaa ccagaccaac gacaccgtca ctgcacgtgg caaaacgcgc
     3841 cataccgggc tggaaacgca ggcacgttac gatctgggta cgctaacgcc aacgcttgat
     3901 aacgtttcca tctacgccag ctatgcgtat gtgaacgcgg aaatccgcga gaaaggcgac
     3961 acctacggca atctggtacc attctccccg aaacataaag gcacgctggg cgtggactac
     4021 aagccaggaa actggacgtt caatctgaac agcgatttcc agtccagcca gtttgcggat
     4081 aacgccaata cggtgaaaga gagcgctgac ggcagtaccg gccgcattcc cggcttcatg
     4141 ctctggggcg cacgcgtggc gtatgacttt ggcccgcaga tggcagatct gaacctggca
     4201 ttcggtgtga aaaacatctt cgaccaggac tacttcatcc gctcttatga cgacaacaac
     4261 aaaggcatct atgcaggcca gccgcgcacg ctgtatatgc aggggtcgtt gaagttctga
     4321 tgtgttaacg cccggcttgc cgggctttta gctggaatgt gattatgttg gcatttatcc
     4381 gttttctttt tgcaggcctg ctgctggtga tcagccacgc ctttgccgcc atggttcagg
     4441 acgaacacgg cacgtttaca ctcgaaaaaa cgccacaacg gattgtggtg ctggaactct
     4501 cgttcgccga tgcgctggcc gccgtggacg tcagcccgat cggtattgcc gacgataacg
     4561 atgcaaaacg catcctgccc gaagtgcgtg cgcacctgaa accgtggcag tccgtcggaa
     4621 cgcgcgcgca gccgagcctg gaagccattg ccgctctgaa accagacctg atcattgccg
     4681 acagcagtcg ccatgcgggg gtttacatcg ccttgcagca aatcgcgccg gtactgctgc
     4741 ttaagtcccg caacgaaacc tacgctgaaa atttgcaatc tgcggctatc atcggcgaaa
     4801 tggtgggtaa aaagcgagag atgcaggcac gtctggaaca acataaagag aggatggcgc
     4861 agtgggccag ccagcttccc aaagggacac gcgtggcctt tggcacatca cgcgaacagc
     4921 aattcaacct gcatactcag gagacctgga ccggcagcgt gctggcctct ctggggctga
     4981 acgttcccgc tgcgatggcg ggtgcgtcca tgccgtccat cggcctggag caactgctgg
     5041 cggtcaatcc tgcctggctg ctggttgccc actatcgcga agagagcatt gttaaacgct
     5101 ggcaacaaga tccgctctgg cagatgttaa ccgccgcgca gaagcagcag gttgcttcgg
     5161 tcgacagtaa cacctgggcg cggatgcgcg gtatttttgc tgcagagcgt attgccgctg
     5221 acacggtaaa aatcttccac catcagccgc ttaccgttgt gaaatgaccg cgataaaaca
     5281 cccggtgctg ctgtgggggc ttcccattgc agcacttatt attattttct ggctgagtct
     5341 gttttgctac tcggccattc ctgtttccgg agcagatgca acccgcgccc tgctgcctgg
     5401 acacacgcca acgctaccag aagcgctggt gcaaaacctt cgtttgccac gaagcctggt
     5461 cgccgttctg atcggcgcaa gcctggcgct cgcgggcacg ctgctgcaaa ccctgaccca
     5521 caacccaatg gcctctcctt cactgctcgg cattaacagc ggcgcggcgc tggctatggc
     5581 gcttaccagc gcgctaagtc cgacgccgat tgcaggctat tctctgtcgt tcatcgcggc
     5641 atgcgggggc ggcgtgagct ggctgctggt catgaccgca ggaggcggat ttcgtcatac
     5701 ccatgacaga aacaaactga tcctcgcggg tatcgcgctg tcggcctttt gtatgggcct
     5761 tacccgcatc accctgctgc tggccgaaga tcatgcttac ggcatctttt actggctggc
     5821 aggcggagtg tcccacgccc gctggcagga tgtctggcag ctcttgccgg tggtggtcac
     5881 tgcagtccct gtcgtgttgc tgctggcgaa tcaactgaac ctgctcaacc tcagcgacag
     5941 caccgcccat acgctgggag tgaacctgac gaggctacgt ttggtcatca atatgttagt
     6001 gctgcttctg gttggtgcgt gcgtcagcgt ggcaggtccg gtggcgttta tcggtctgct
     6061 ggtgccacat ctggcgcgct tctgggcagg cttcgatcag cgcaacgtac tgccagtgag
     6121 catgctgctg ggggccacgc tgatgctgct ggcagatgta ctcgcacgcg cgctggcctt
     6181 ccccggagat ctgcccgcag gcgcagtgct ggcgctgatt ggcagccctt gctttgtctg
     6241 gcttgtgagg aggcgaggat gaaaattgcg ctggttattt tcatcaccct tgccctggcg
     6301 ggctgtgcgc tgttatcact ccatatggga gtgatccccg tgccgtggcg cgcgctgctg
     6361 accgactggc aggccggacg cgagcattat tatgtattga tggagtaccg actgccgcgc
     6421 ttgctgctgg cactgtttgt cggtgcagcc ctcgccgtgg cgggcgtgct gatacagggg
     6481 attgtgcgca accctctggc atcaccggat attctcggcg ttaaccatgc cgccagcctg
     6541 gcctctgtgg gggctctact tcttatgccg tcactgcccg tgatggtgct gccgctgctg
     6601 gcctttgcgg gcggcatggc ggggttgata ttactgaaga tgctggcaaa gacccaccag
     6661 ccgatgaagc tggcgctcac cggcgtggcg ctttctgcat gctgggccag cctgacggat
     6721 tatctgatgc tctcgcgccc gcaggatgtg aacaacgccc tgctgtggct gaccggcagc
     6781 ttatggggcc gtgactggag ctttgtgaag attgccatcc cgctgatgat tttatttctg
     6841 ccgctgagcc tgagtttttg ccgcgatctc gacctccttg cactcggcga tgcgcgcgcc
     6901 accacgctcg gtgtgtcggt gccccatacc cgattctggg ctttgttact agctgtcgcc
     6961 atgacatcta ccggcgtggc cgcctgcggc ccgattagct ttattggtct cgtggtgccg
     7021 catatgatgc gtagcatcac cggtggacgt caccgcagac tgctgcctgt ttcggccctg
     7081 acaggtgcgt tgctgttggt ggttgccgat ctgctggcga gaattattca tcccccactg
     7141 gagctcccgg ttggcgtgct gaccgccatt atcggtgcgc cgtggtttgt ctggttgctt
     7201 gtgagaatgc gataaatgac tttacgaact gaaaatctga cggtcagtta cgggacagac
     7261 aaggtactta acgacgtttc actctcactg ccaacgggga agatcaccgc cctgatcggt
     7321 cctaacggtt gcgggaaatc gacgctgtta aactgttttt cgcggctttt aatgccgcag
     7381 tctggcaccg tatttctcgg cgataatccc ataaatatgc tctcatcacg ccagttggcc
     7441 cgcaggcttt cgctgctgcc tcagcaccat ttaacgccag aggggatcac agtccaggag
     7501 ctggtttcgt atggtcgtaa tccctggctg tcactctggg ggcgtctctc cgctgaagac
     7561 aatgcacgag ttaatgtcgc catgaaccag acccggatca atcatcttgc cgttcgtcgg
     7621 ttaaccgagc tttccggcgg tcagcgccag cgcgcatttc tggcgatggt cctggcccag
     7681 aatacgcccg ttgtattact tgatgagcca accacctatc ttgatatcaa tcaccaggtg
     7741 gacctgatgc ggttgatggg cgaactccgg actcagggga aaacggtggt cgctgtgctg
     7801 cacgacctta atcaggctag ccggtactgc gatcaactgg tggtaatggc aaacggacat
     7861 gttatggcgc aaggcacacc agaagaggtg atgaccccag gattgctgag aacagtattc
     7921 agcgtggaag cggaaataca ccccgagccg gtatctggca ggccgatgtg cctaatgaag
     7981 tagattgcac aggccgtaag aaccaaacca cgactgaatg aaactggact ggcgccagca
     8041 agcctgttca aactggggct gaacttttcc ggactctgaa agattaccaa tactcatcgt
     8101 ccatccgctt gctttaggct gacaggttca taattaacgc aaaccagagc tgtacaggct
     8161 tgggcgcggc tttcaaacca gtcgtgatca cggcaatcaa ttttgaactc tgcttaacgg
     8221 acatttctgt ataaccctta cggcaacgaa aaacgcgaag ttaaaatttt agaaacccaa
     8281 aaacgtgaca tgactaagtt tagatttcag gggggggaga tcaaaaaatt tcgctctgtg
     8341 ccagagcgga cattcacgga gctggttcat taccaatgag gttgggcttt tgaggataaa
     8401 tcaatgatca gacgccaacg taaatcaaaa gcaaccctgg aacggaattg ctaatccagt
     8461 ttctgaccat cgatttttct aaaaagtgtg cgtttgctac tacttaggta ggtgcagctt
     8521 tcttaatcac cggcagccac gttatacagg ccagttgatg gatcgattgt tatcaatgat
     8581 atcttcatga gtcggtgtct cacccagctt accgaagctg gcataaagtg aaggcagacg
     8641 ggcccgtcct tctccctttt tcgccagagg gaaagcgcga agcatggtgg cagcctccca
     8701 gtcacaacaa taggatggtg tgcacggctg ctgacgccat gattcagcga tagagccgga
     8761 aaatacgggg tcaaagccgg tatcattaac cagagtcatc gtgacctgta ctgcggctgg
     8821 atgatctcct gctactgcaa ctgctaggcg accgcggctc ccttcaggta gtctgttgtg
     8881 gtcaactaaa actggcctcc gcgttagagt ttttccagta tcggttttct gattcgtttg
     8941 gtggtaaccc accattatat tcgtgcggtc ttagtgcgct gtaatatcca acgatatagt
     9001 ccgttattgc gtgagctgca tcgctgaagc ttacataccc cgtcgccggc acccattcgt
     9061 tcttcagact cctgaagaag cgctccattg ggctgttatc ccagcagttt ccacgccgac
     9121 tcatactctg cctgatccgg tatcgccaca gtaattgccg gaactgcctg ctcgtataat
     9181 gactgccttg atcgctgtgg aacatcaccc cgacgggctt accacgggtt tcccatgcca
     9241 tttccagtgc tttcatggtg agcctgctgt ccggcgagaa cgacatggcc cagcccactg
     9301 gttttcttgc gaacaggtcg agaacaacgg cgaggtacgc ccagcgctta cccgtcc
//
LOCUS       DAZGJT010000140         9341 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZGJT010000140 DAZGJT010000000
VERSION     DAZGJT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9341
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.143"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            139..1221
                     /locus_tag="ACHKFK_004245"
     CDS             139..1221
                     /locus_tag="ACHKFK_004245"
                     /EC_number="1.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008888 - glycerol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HFO9054463.1"
                     /translation="MDKIIISPAKYIQGNGSLDNIATYAASLGTEPLIIADEFVTGLV
                     GDRVSQSFARENIIADFDVFCGECSQNEISRIRKKFNQRKYNVVIGIGGGKTLDTAKA
                     VAYYQKIPVVVVPTIASTDAPTSSLAVIYTPEGQFSEYLFFPKNPDMVIMDTGVISAA
                     PVRLLVAGMGDALSTWFEARANQASGKATMAGGASTLAALAIARLCYTTLLEDGYKAK
                     VAVEQGVSTKAVENIIEANTYLSGIGFESSGLAAAHAIHNGMTQLEECHHCYHGEKVA
                     FGVLVQLVLENAPQEEIETVLNFCHSVGLPTNLHMLGVKEINKDKLRAVAEAATAEGE
                     TIHNMPFVVTAQDVLCAILTAHHLGL"
     gene            1288..2556
                     /locus_tag="ACHKFK_004246"
     CDS             1288..2556
                     /locus_tag="ACHKFK_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9054464.1"
                     /translation="MTSNWKRWITLALCGIAGSAIYKLPYLRETYYDAMQQATGATNA
                     ELGFLMTAYGLVNFLLYLPGGWAADRFSARKLMTFSLISTGISGFYYATFPSYTMICL
                     LHALWAVTTVFTFWAVCVRIIRTLGTSEEQGRLYGYWFLGKGLTSIVLGFLSVPVFAK
                     FGEGVDGLRATIIFYSVVTILAGVLAWFVCQDETHSEDKANFRLADMAFVLKMPTVWL
                     AGVVTFCMWSIYIGFGMVTPYLTQILHMGESEVAVASILRAYVLFAMGGLIGGQLADR
                     CASRTRFMIYAFIGMIVFTTVYFFLPGESRYVTIALANMVALGVFIYSANAVFFSIID
                     EVRIPAKVTGTAAGLISLLTYFPEIYCYTMVGNMVDRKPGIAGYQDVFLFMLVCAFIG
                     LIAALVLQRVNRKSKNQVNEITENNADCAS"
     gene            2574..3938
                     /locus_tag="ACHKFK_004247"
                     /pseudo
     CDS             2574..3938
                     /locus_tag="ACHKFK_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228116.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            3991..4215
                     /locus_tag="ACHKFK_004248"
     CDS             3991..4215
                     /locus_tag="ACHKFK_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054465.1"
                     /translation="MRDDDLEHLSANYWITTVLCGLFLGIFLFMLQQHQRGLALYCIA
                     ALSLICAFFSLRLSRQLQHMKKERRNIRPH"
     gene            4239..5780
                     /locus_tag="ACHKFK_004249"
     CDS             4239..5780
                     /locus_tag="ACHKFK_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO9054466.1"
                     /translation="MQYLKKRFSLIELNVFIPAILFIAVIILCLTIYPQDTSRYINKI
                     HHFLTWEMGGIFLVMTFLVVLCCLWLAFSRYGDILLGQSGEKPDFSLLTWLGLIFTSG
                     TGGSLLYLASVEWIWIIQQPPFGATAGSAQAARWASAYGMFHWGPSAWAWYLICAVPI
                     GWFMHVKKTNSLKVSDLCRGCLGARADGFCGHCVNFFYMFGLLGGAVTSLALGTPMIS
                     AVFCHVFHLDPAGQFINVMVIFIWTLVPLFILFFGLKKGVAWTSNWNIRADILMLLAI
                     LICGPTAFILNQSIDGLGLMLQNFVAMSLSTDAIGRSGFPQMWTVFYFSWWVVYAIPF
                     GLFIARISKGRTIRQLIVCGTLAGSLGCMVFYMVLANFGLSLQTTHVIDFVPILNEQG
                     RGVVVSRLLEQLPASQVFLVAFGAIALISYITGHCTVGYALGFATQKRADSESEPAFW
                     NVAFWLIMTGIVAITLYLLDAQSLQPLQTVSILAGLPLCGVVFILLKSFLTQLAAEEK
                     NARDE"
     gene            complement(5836..6978)
                     /locus_tag="ACHKFK_004250"
     CDS             complement(5836..6978)
                     /locus_tag="ACHKFK_004250"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFO9054467.1"
                     /translation="MKIVISPDSFKECLPAWKVAEALATGWRKVLPGSQLVCLPVADG
                     GEGTLETLIHATDGTFYTKKVTGPLGESIHAQYGILGNQTTAVIELAQASGLELVSPV
                     QRSPLYTTSFGTGELILAALEHNIDTVILCLGGSATNDGGIGLMSALGASFTDAEGLS
                     VSVNGMGLAAIHHIDLQHLDPRLKNVKFIAACDVTNPLTGDNGATRVFGQQKGASADD
                     LEQLEQGMKNYARCIYRCCGKEVDTIPGSGAAGGVGAALMAFLDARLQPGISLVLEAI
                     QYTQHLKYAALAIVGEGKLDSQSLNGKAPVGAAKVAQMMGVPVIAIAGYIDDQLDLNE
                     LRQCGIEACFSVVNGPCDLPTALSQGESNLIHLGENLAGYFRAILS"
     gene            complement(7053..7940)
                     /locus_tag="ACHKFK_004251"
     CDS             complement(7053..7940)
                     /locus_tag="ACHKFK_004251"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0019580 - galactarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9054468.1"
                     /translation="MNIGFIGTGIMGKPMAYNLQQAGHTLYFSAHFEPAPQELIGERG
                     IVCSTPREVAQECEVIITMLPDTPHVEDVLFHPNYGVIHGLSHGKIVIDMSSISPVAT
                     KAFAQRIIAGGAEYIDAPVSGGEVGAKAGTLSIMVGGCEEVYLQIKPILELMGKNITL
                     VGNVGDGQTCKVANQIIVALNIEAVAEALLFASKSGADPARVREALMGGFASSRVLEV
                     HGERMIKGTFEPGFRISLHQKDLNLALENARLLNTPLPNTATTQQLFSACAALGGKEW
                     DHSALIRALETQANFTIRK"
     gene            complement(7958..8740)
                     /gene="hyi"
                     /locus_tag="ACHKFK_004252"
     CDS             complement(7958..8740)
                     /gene="hyi"
                     /locus_tag="ACHKFK_004252"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001682525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO9054469.1"
                     /translation="MAKFAANLSMLFTEYPFIERFAQASHAGFHGVEYLFPYDFSADE
                     LVSQLHQHNLTQVLFNLPAGNWQEGERGIACHPGRAKEFQEGVSRAIEYALALNCSQV
                     NCLAGKHPGGYSHEQCHETLVENLRYAVDKLASHGIKLVLEAINTKDIPGFFVNNTRQ
                     ALNIIHDVNHPDFRYQYDIYHMQIMEGNIATTIKNNLNNIGHIQLADNPGRHEPGTGE
                     INYPWLLNYIDQIGYQGWIGCEYIPSTTTTESLRWLKNETQF"
     gene            complement(8753..>9341)
                     /locus_tag="ACHKFK_004253"
     CDS             complement(8753..>9341)
                     /locus_tag="ACHKFK_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HFO9054470.1"
                     /translation="PETRYISTIGLAQIAANQFLHVYRPRHWINACQAGPLGWTMPAA
                     LGAVKADPSVPVVAISGDYDFQFLIEELAVGAQFNLPYIHILLNNAYLGLIRQSQRAF
                     DIDYCVQLSFENINAPEINGYGVDHKAVVEGLGCKAIRVFASQDIAPALQEAQRLRDE
                     FHVPVVVEIITERVTNIAMGPDINKVTEFEEILDL"
BASE COUNT         2341 a         2031 c         2340 g         2629 t
ORIGIN      
        1 tatttttatt attgctttgg gatgatgttt tttacagcgc gatcataaaa cataattttt
       61 cgcgaatgaa atgtttgccg ccactggatg caacgagtag gttaacagtg aagcaaaaca
      121 agttacgcga ggaatgatat ggataagatt attataagtc cggctaaata tatccaggga
      181 aatggttctc tggataatat tgctacatat gcggcgagtt tgggaacaga accattaatt
      241 attgcagatg aatttgtgac cggtctggtg ggtgacagag taagccagag ctttgcaaga
      301 gaaaacatta ttgcggattt tgatgttttt tgcggtgaat gctcgcaaaa tgaaatttct
      361 cgaattcgta agaaatttaa tcagcgtaaa tataatgtcg ttattggtat tggcggcggt
      421 aaaacgttgg atacggcaaa agccgtagcg tattaccaaa aaattccggt agtggtggtg
      481 ccgacaattg cttccacaga tgcgccaacc agttctctgg cagtaattta caccccggaa
      541 ggccagttta gcgaatatct gtttttcccg aaaaacccgg atatggtgat tatggatact
      601 ggcgtcattt cagccgcgcc agtgcgtctg ctggtggccg gtatgggaga tgcattgtca
      661 acctggtttg aggcgagagc caatcaggcg tcaggaaaag ccacaatggc aggtggggcg
      721 tcgacactgg cggcactggc tattgcccga ctctgttata ccacgctact ggaagatggc
      781 tataaggcaa aagtcgcggt tgaacaaggt gttagcacca aagcggtaga gaatattatc
      841 gaagctaata cctatcttag cggcattgga tttgaaagta gcggcctggc ggcagctcat
      901 gccattcata acggcatgac gcaactggag gaatgccatc attgctacca cggtgaaaaa
      961 gtggcatttg gcgtcctggt acagctcgta ctggaaaacg caccacagga ggaaattgaa
     1021 accgtactca atttctgcca tagcgttggt ctgccgacaa atcttcatat gttaggcgtg
     1081 aaggaaatta ataaagataa attacgggca gtggctgaag ccgcgactgc tgaaggggaa
     1141 actatccata atatgccatt cgtggtcact gcgcaggacg ttctttgcgc aattttaact
     1201 gcacatcatt tgggacttta aaaaataata cggcctgtgc aaagcaggcc gttggtgtca
     1261 ttttaaatac ttcattcggg aataaatatg accagtaact ggaaacgttg gatcacgctg
     1321 gcgttatgtg gtattgccgg aagtgcaata tataaattgc cttatttacg ggaaacctat
     1381 tacgacgcga tgcagcaggc tacaggagcg actaatgctg aacttggttt tctaatgaca
     1441 gcctatggtc tggtgaactt tttattatat ctacctggcg gctgggctgc cgatagattt
     1501 tctgcacgta aattaatgac cttttcatta atttccaccg ggataagcgg tttttattat
     1561 gcgaccttcc cctcgtatac catgatctgt ttgttacatg cactttgggc tgttacaacc
     1621 gtatttacgt tttgggccgt gtgtgtgcga attattcgta ccctgggaac cagcgaagaa
     1681 caaggacgct tatacggtta ctggtttctg ggtaagggat taacctccat tgtactggga
     1741 tttctttccg tgccggtatt tgccaaattt ggcgaaggtg ttgatggttt acgggcgaca
     1801 attatttttt attccgtagt gactattctg gccggtgttt tagcctggtt tgtttgtcag
     1861 gatgaaacac acagcgaaga taaagcgaat ttccgtctgg cagatatggc attcgtgctg
     1921 aaaatgccga cggtttggct ggcaggtgtt gtgacctttt gtatgtggtc aatttatatc
     1981 ggctttggca tggtcacgcc gtatctcacg cagatcctgc atatgggcga atcggaagtg
     2041 gcagttgcca gtattcttcg cgcctatgtg ttatttgcga tggggggatt aattggcgga
     2101 caactggccg accgctgtgc gtcacgcacg cgttttatga tttacgcctt tatcggcatg
     2161 attgtcttta ctaccgttta tttcttcctg cccggtgagt cgcgctacgt cactattgcg
     2221 ctggcgaata tggttgcgtt gggcgtgttt atttactctg cgaatgccgt gttcttctca
     2281 attattgatg aggttcggat cccggcaaaa gtcacaggga ctgctgcggg tctgatctct
     2341 ttactgacct atttcccgga aatctactgc tacacgatgg tgggcaatat ggtcgaccgc
     2401 aaaccgggta ttgccggtta tcaggatgtt ttcctgttca tgttggtttg cgcattcatc
     2461 ggccttatcg ccgcactggt cctccagcgt gtgaatcgta aaagtaaaaa tcaagtcaat
     2521 gaaatcactg aaaacaatgc ggattgcgca tcctgaatct gacgggggaa ccaatggtgg
     2581 acatgattaa tgaaagtgca cggcaaacgc cagtcattgc acaaacggac gttctggtta
     2641 tcgggggcgg tccggcagga ttatccgctg ccattgcggc agggcggtta ggtgccagaa
     2701 ccatgattgt tgagcgctac gggtcgctag gcggcgtatt gacgcaggtc ggggtagaaa
     2761 gttttgcttg gtatcgtcat tcggggacgg aagattgtga agggatctgt cgtgagtatg
     2821 aaggccgcgc acgagcgctg ggtttcacac gaccagaacc tcagtcaatt agcgaagtta
     2881 tagatactga aggatttaaa gttgtcgccg atcagatgat tacggaatct ggcgttgagc
     2941 cgttatatca ctcctgggtt gtggacgtga tcaaggacgg gaatacgtta tgcggtgtta
     3001 tcgtcgagaa taaatcaggt cgaggggcaa ttctggcgaa aagaatcgtc gattgcacgg
     3061 gggatgctga tattgccgct cgtgcaggcg cgccctggac gaaacggagc aaggaccaac
     3121 tgatgggcgt caccgtgatg ttcagttgcg caggtgttga tgtggcacgc tttaaccgtt
     3181 ttgttgcgga agaacttaag ccgacctacg cggattgggg caaaaactgg acgattcaaa
     3241 ccacgggtaa agaagacccg atgtttagcc cgtatatgga ggatattttt acccgcgcgc
     3301 aacaggatgg tgtgattcca ggtgacgccc aggcgattgc cggaacttgg tcgacctttt
     3361 ctgaaagcgg tgaggctttc tagatgaata tggtgtacgc ctttggtttt gactgtaccg
     3421 atgtcttcga tttaaccaaa gctgagattg ccggaaggca gcaagcatta tgggcaattg
     3481 acgcactacg ccactatgtt ccgggctttg aaaatgtacg gttacgcaat tttggtgcca
     3541 cgctggggac gcgtgaatca cggcttattg agggggaaat acgtattgct gatgattacg
     3601 tccttaatca ggggcgttgt tcggacagtg tagggatttt cccggaattt attgatggtt
     3661 ccggttatct cattttgcca acgaccgggc gtttctttca gatcccttat ggttgtctgg
     3721 tgccgcaaaa agtggagaac cttttggtcg ccggtcgctg tatttccgca ggcgtagttg
     3781 cacatacttc tatgcgtaac atgatgtgtt gtgccgttac cggtgaggcc gcaggtactg
     3841 ccgccgtggt ttcgctacag caaaattgca ccgtgcgtca ggttgctatc cctgatttgc
     3901 aaaacacgct gcaacagcag ggcgtacgtc tggcataaca acaaggcgca gagagctgaa
     3961 agcctgtctg cgccttgcag gagaaagacc atgagggatg atgacctgga gcacttaagt
     4021 gccaattact ggataaccac agttctctgc ggcctctttt tggggatatt cctgttcatg
     4081 ctgcaacagc atcagcgtgg tttagctctg tattgtattg cggctctttc acttatttgc
     4141 gccttttttt ctttacgcct ttcccgacaa ttgcaacaca tgaaaaagga acggcgaaat
     4201 attcgtcctc attgaatgaa aacattcagg agactgaaat gcaatattta aaaaaacgct
     4261 tttctttgat tgaactgaat gtgtttatcc cagcaatatt atttattgcg gtaatcattc
     4321 tgtgtttgac gatatatcca caagatacca gccggtatat aaataaaata catcattttt
     4381 taacctggga aatgggcggg atttttctgg tcatgacttt tctggttgta ctttgttgtc
     4441 tgtggctggc cttctcacgt tatggcgata tcttgttagg tcagtcggga gaaaaacctg
     4501 acttcagctt gttaacctgg cttggactta tttttacttc tggaacgggg ggcagcttgt
     4561 tatatctggc ctctgtagag tggatttgga tcattcagca accgcctttt ggcgcaacag
     4621 caggaagcgc tcaggctgct cgttgggcct ctgcttatgg catgttccat tgggggccgt
     4681 ccgcatgggc atggtatttg atttgcgccg ttcctattgg ttggtttatg catgttaaga
     4741 aaacaaactc attaaaggtc agtgatttat gtcgtggatg tttgggagcg cgtgcggatg
     4801 gtttttgcgg gcattgtgtg aattttttct acatgtttgg tttgctcggc ggcgcggtaa
     4861 cgtctctggc gctgggaacg ccgatgattt ctgccgtatt ttgccatgtg ttccatctgg
     4921 atcctgccgg gcagtttatc aatgtcatgg ttatttttat ctggacgcta gtaccattat
     4981 tcattctctt ttttggactt aaaaaaggtg tggcatggac cagtaactgg aatattcgtg
     5041 ctgacatttt gatgctactg gcaatactga tttgtggacc aacggcattt atacttaatc
     5101 aatcaattga tggtctcggc ctgatgctgc aaaattttgt agcgatgagt ttaagtaccg
     5161 atgctattgg tcgtagcgga tttccacaga tgtggaccgt attttatttt tcatggtggg
     5221 tcgtgtatgc catcccattc ggtttattta tcgcccgtat ttcaaaagga aggacgatcc
     5281 ggcaattgat tgtatgtgga actctggcag gttcattggg atgcatggtt ttttacatgg
     5341 tactggctaa tttcggctta tcgttgcaga caactcatgt catcgatttt gttcccatac
     5401 ttaacgaaca agggcgaggc gttgtcgttt ctcgtttact ggagcagcta cccgcaagtc
     5461 aggtgttttt ggttgctttt ggggctatag cattaatttc atatattacc ggacactgta
     5521 ctgtgggtta tgccctcggt tttgcgacgc aaaaacgggc agatagtgag agtgaaccgg
     5581 cattctggaa cgtggcattt tggttgatta tgactggaat cgtcgccatc acactctatc
     5641 ttcttgatgc gcaaagtctg caaccgctac aaacggtgtc tattctggct ggactaccgc
     5701 tttgcggcgt ggtgtttatt ttattgaaga gttttttgac gcagcttgcg gctgaagaga
     5761 aaaacgcgag agatgaataa tgatgttcag gaatgcccgg taatttgcat tatcgggcat
     5821 tttaccgaac gggaattaac tcaggatcgc acgaaaatat cccgccagat tttccccaag
     5881 atgaattaaa ttactttctc cctgactcag cgcggtgggt aaatcacaag gaccattgac
     5941 gacggaaaaa caggcttcta ttccacactg gcgtaactca ttcagatcaa gttgatcatc
     6001 gatatacccg gcaattgcga taaccggaac gcccatcatc tgggcaactt tggctgcgcc
     6061 tacgggcgct ttgccattca ggctctgact atctaatttc ccttcaccga caatggccaa
     6121 tgctgcgtat tttaaatgtt gggtatattg aatcgcttcc agcacgagcg aaatacccgg
     6181 ttgtaagcga gcatcgagaa aagccatcaa ggccgcgcca acgccgccag ccgccccaga
     6241 accgggtatc gtatcgactt ctttaccaca acaacggtag atgcaacggg catagttttt
     6301 cattccctgt tccagttgct caaggtcgtc agcactggcc cctttttgtt gaccgaaaac
     6361 ccgggtcgcg ccgttatcgc cggttagtgg gttggtgaca tcgcaggctg caataaattt
     6421 cacatttttc aatcgtggat cgaggtgctg taagtcaatg tggtgaattg ccgccagccc
     6481 catcccatta acagagactg atagcccttc ggcgtctgta aacgatgcgc caagtgccga
     6541 catcaaccca ataccgccat catttgtagc actgccaccc aggcatagaa taacggtatc
     6601 aatattgtgt tccagggcgg cgagaataag ttcgccagtg ccaaacgacg tcgtataaag
     6661 gggagaacgc tggacaggag aaaccagttc cagccctgaa gcttgtgcca gttctatcac
     6721 tgcggtggtt tggttgccta aaataccgta ttgcgcatgt attgattcgc caagcggtcc
     6781 ggtgactttt ttagtgtaaa acgtaccgtc agtcgcatga atgagtgttt cgagcgtgcc
     6841 ttcgccgccg tcagccacgg gcaaacacac cagttggctg ccaggcagga ccttgcgcca
     6901 gccggttgcc agcgcttcgg cgactttcca tgcaggaagg cattctttaa aggagtcggg
     6961 ggagataact atcttcatag caggtctctc aaaatagggg aatggggcag ggcaatgagt
     7021 gaacgaaggg aaccggtggt tcccttaatt cattatttac ggatagtgaa attggcttgt
     7081 gtttcaagcg cccgaattaa cgcagaatga tcccactctt taccaccgag cgcggcgcag
     7141 gcgctaaata actgctgtgt cgtcgccgtg ttaggaagcg gggtattcaa caggcgggca
     7201 ttctccagcg ccagattcag atctttttga tgcagactaa tacgaaaacc gggttcgaat
     7261 gttcctttaa tcatccgttc gccatgaact tccagcacgc gagaggaggc aaaccctccc
     7321 atcagcgctt cacgtacgcg ggcgggatcg gcacctgatt tactggcaaa gagcagggct
     7381 tcagccaccg cttcaatatt cagtgcaaca ataatttggt tagcgacttt gcacgtttga
     7441 ccatcaccga cgttaccgac cagagtgata tttttaccca tcaattccag aatcggtttg
     7501 atctgcaaat aaacctcttc acatccgcca accataatgg aaagcgtccc ggctttcgcg
     7561 ccaacttcac cgccagagac gggcgcatcg atatattccg cgcctcccgc gataatacgt
     7621 tgtgcaaatg ctttggtggc aaccggggaa attgaactca tatcaatcac aattttcccg
     7681 tggcttaaac cgtgtattac gccatagttg ggatgaaaaa gaacatcctc aacatgtggg
     7741 gtatcaggga gcatggtgat gatgacctca cactcctgcg cgacttcacg cggtgtagag
     7801 caaacaatgc cacgttcacc aataagctct tgcggtgcag gttcaaaatg cgctgaaaaa
     7861 taaagagtat gtccggcttg ttgtaaatta taagccatcg gcttacccat tatgccggtg
     7921 ccgataaatc caatattcat gataaatacc tcagggatta aaattgagtc tcgtttttta
     7981 gccagcgtag actttcagtc gttgtcgtag atggaatata ttcgcaacca atccatccct
     8041 gataaccaat ctggtctata taatttaaca accacggata attaatttct cccgtccctg
     8101 gttcatgacg cccagggtta tcggctaatt gaatatgtcc aatgttattc aggttgtttt
     8161 tgattgttgt agcaatattc ccttccatga tttgcatatg ataaatatcg tattgatagc
     8221 gaaaatcagg atgatttaca tcatgaataa tattgagagc ttgtctggtg ttgttaacaa
     8281 aaaaaccggg aatatctttt gtattaatgg cttccagaac taactttatg ccatgagaag
     8341 ccaatttatc tactgcatag cgcaaatttt cgaccagtgt ttcatgacat tgttcatgcg
     8401 aataaccacc tggatgtttt cctgcaaggc aattaacttg tgaacaatta agggccagtg
     8461 catactctat tgccctggaa acgccctcct gaaattcttt tgcccgacca ggatgacatg
     8521 caataccccg ttcaccctct tgccagttac ccgcaggaag attaaacaga acctgcgtca
     8581 gattatgttg atgcagttgc gaaaccaact catctgcgga aaagtcatag gggaaaagat
     8641 actcaacgcc gtggaatcct gcgtgacttg cctgtgcaaa acgttcaata aatggatact
     8701 cggtaaagag catagataaa tttgctgcaa actttgccat aatcctgttt ccttataaat
     8761 cgagaatttc ttcaaattct gtgactttat taatgtcagg tcccatagcg atattagtca
     8821 cgcgttcagt aataatttct acaacaacag gtacgtgaaa ttcgtcacgc aaacgctggg
     8881 cttcttgcag tgcaggcgca atatcctgtg atgcgaatac gcgaatcgct ttacatccta
     8941 atccttcaac cacagcttta tgatcaacgc cataaccatt aatttctgga gcattaatat
     9001 tttcaaatga cagttgaacg caataatcaa tatcaaatgc acgttgtgat tgacgaatca
     9061 aaccaagata agcgttgtta agtaaaatat ggatatacgg aagattaaat tgtgcgccga
     9121 cggctaattc ttcaatcaag aattgaaaat cgtaatcacc ggatattgcc acaacgggaa
     9181 cagatggatc agcttttacc gcgcccagtg ctgcgggcat tgtccagcct agcggcccag
     9241 cctgacaggc attaatccag tggcgtgggc ggtaaacatg taaaaattgg tttgcggcga
     9301 tttgcgccag accaattgtc gaaatatagc gtgtctccgg t
//
LOCUS       DAZGJT010000141         9313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZGJT010000141 DAZGJT010000000
VERSION     DAZGJT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.144"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..229
                     /locus_tag="ACHKFK_004254"
     CDS             <1..229
                     /locus_tag="ACHKFK_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO9054471.1"
                     /translation="IQMFSDHLLSVYNFSNLRDYLSNELPHLKKLDEFLNSYLNELSI
                     SFRRGAIIELKNTVKKVKESLENSISFINKN"
     gene            complement(500..1072)
                     /locus_tag="ACHKFK_004255"
     CDS             complement(500..1072)
                     /locus_tag="ACHKFK_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage polarity suppression protein"
                     /protein_id="HFO9054472.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(1146..1646)
                     /locus_tag="ACHKFK_004256"
     CDS             complement(1146..1646)
                     /locus_tag="ACHKFK_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO9054473.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDSKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(1643..2377)
                     /locus_tag="ACHKFK_004257"
     CDS             complement(1643..2377)
                     /locus_tag="ACHKFK_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HFO9054474.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETITAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGGCDSTATAYRKAHHHLLSLYAEHDLEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            2928..3194
                     /locus_tag="ACHKFK_004258"
     CDS             2928..3194
                     /locus_tag="ACHKFK_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9054475.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEITAWMAGRIAGRKRGYDA"
     gene            3191..3781
                     /locus_tag="ACHKFK_004259"
     CDS             3191..3781
                     /locus_tag="ACHKFK_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO9054476.1"
                     /translation="MMMPALQKVPFSGLPFSGICGYSFSAAAKSAAGRRNPCNSKATY
                     DAPCVFFCVAINATERQIMVWRVVCRAGMILFAIACYATESMVAQAGQPPGWPVFFEA
                     GIPTPVWAIAIERRNSGDSSYLLLEGDCLMATTLTPSHPEFVFVFAAVRRADRHPRIC
                     MLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            3774..4061
                     /locus_tag="ACHKFK_004260"
     CDS             3774..4061
                     /locus_tag="ACHKFK_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HFO9054477.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSRFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            4054..4509
                     /locus_tag="ACHKFK_004261"
     CDS             4054..4509
                     /locus_tag="ACHKFK_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054478.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPEIVWNP
                     YRRKFSILVRILTDGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYLDIYPQDTVPASRAAPVAREWREISRTEPDPEITPDNSYRNYL"
     gene            4645..4965
                     /locus_tag="ACHKFK_004262"
     CDS             4645..4965
                     /locus_tag="ACHKFK_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HFO9054479.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACTLLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            4980..7313
                     /locus_tag="ACHKFK_004263"
     CDS             4980..7313
                     /locus_tag="ACHKFK_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primase-helicase zinc-binding domain-containing
                     protein"
                     /protein_id="HFO9054480.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGSLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLENHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            7871..8908
                     /gene="rhuM"
                     /locus_tag="ACHKFK_004264"
     CDS             7871..8908
                     /gene="rhuM"
                     /locus_tag="ACHKFK_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HFO9054481.1"
                     /translation="MSDKDLTQPPAGEFIMFASGDGRVRVECRFESDTIWLSQAAMAE
                     LYDKDVRTINEHLINIFSEGELVQNSTIRKFRIVRQEGKRQVSREIDHYNLEAILAVG
                     YRVRSPRGTQFRQWATQTLQEYLIKGFVMDDERLKNPPVGSSVVPDYFDEMLERIRDI
                     RASERRVYLRMREIFALAADYQPSLKETTQFFQTIQNKLHFACTGHTAAELIHKRADA
                     SQPHMGLTSYKGEEVRKGDVTVAKNYLTQDEVSELNRVVNMWLDFAEDQARRRQQVFL
                     RDWQDKLDQFLQFNDREVLQGAGKISKKMADEKAQAEYVQFAEQQRRLKEAEGEKDIA
                     GLLQWNKESKK"
     gene            9116..>9313
                     /locus_tag="ACHKFK_004265"
     CDS             9116..>9313
                     /locus_tag="ACHKFK_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054482.1"
                     /translation="MIRFSCRVYENVFLATDYQPSLKKTSLFFKLSRISCILSAPDTS
                     HPYMAFTCYKDEEVHKCDVKTI"
BASE COUNT         2193 a         2529 c         2404 g         2187 t
ORIGIN      
        1 tattcaaatg ttttcagacc atctattatc tgtatacaac ttttctaacc ttcgcgatta
       61 tctatctaac gagctacctc atctaaaaaa actagatgag ttcttaaatt cttacctgaa
      121 tgaactttca atttcttttc gtcgcggagc cattattgag ttaaaaaaca ccgtgaaaaa
      181 agtaaaggag tcactagaga acagtatttc attcataaat aaaaactgaa cacatccatg
      241 taaaaacaaa aaactctcca gttcagtttc tttctctggc tggagagtca ggttatttcc
      301 tgcatacatg cgcgcagtgc tttccccgcc tcgcccgccc gctttgcggg gcggttttaa
      361 tgcagtttca ctgacacgct caggccgcgc cgggaatggc gcggtctgca gaaaatgagg
      421 caggaaaacg catgcaaagc catgcacctt atcgatgcat ggcttttttc agtaaaaaca
      481 ggcggatttt cggggaattt tacacagact gacgtgatgc cagttgcgca cttttacgcg
      541 aaaaaatcat gttctgcgca ggggtgaatt tttcacggct gtcatccacc gaagccgcgt
      601 caggcctgaa tccgatggtc gttaaaatgt cgctatcctg tgcggaataa ttaatttttt
      661 cccccttcgc aagccaggac aggagggctt cacgcagggc atctgtggca cgctgtatgg
      721 cacagtttcg ggcaatggcc gtcagctcac tgtagcccat cagctccggt gccagtgccg
      781 ccgccagtgc tgtgccgtgc tgctgcataa aatcattcag ccggtcgcgg atgctgatgt
      841 gctgaacggc ttcatgcgaa cgaatataac gaccagcggc ctgattcacc tgccattttc
      901 tgacttcgat aatattgcgt aattcgtcca ggcgactgac gtttctgcct tctcctgaca
      961 gaagccgcag atattcctgt tcggccgctg ccagctcatt tttgcgttgc agccatgctg
     1021 ctttgttatt ctgacaggtg tcaaaggcct gctgtaaggc tgtgctttcc atcgttatct
     1081 ctttctcatc atgctgaaga ataaaaatac ggtgtgcggc gacggccggt gttaaccggc
     1141 agccctcatt ccagacgcag cgaatatgat tgtgttttta agcgtactgg cggcagttcc
     1201 tgtttttcat gcaggctttc tgccagttcg tccggcgtga ccgggcggac aatgaaacgg
     1261 ttgatggtct gaagcgtttt aaacaccaga ccacagcccg gatccgtgca cacataaaaa
     1321 cgctcggtga cttcctgaga cagacgccgc gatgttcttg acagtgcaag gcctttacat
     1381 ctccgacaac aatatccggt aacaagcatt cttttcgggc gtttcatact gccggaggct
     1441 gacgtcagtg aatcgcggta tctctgtttg cctgaaatgt attccattcc tgaatcttta
     1501 ctgtcagaga aaaagctttc actcgcttca aatgtcgcag agcaataaat attccggcac
     1561 tgtgcaatca ttatcttggt gccatcgtcc atgaaatgtg cgcgacgggt gtgagcaaca
     1621 tgtccacacg acgggcagta aatcatgaca gcagtcctct ggccttaagc tctgctccct
     1681 gctggtctat tttgtcctgc cacaccttgc gctgtgccgg tgtgcctgcc acctcataat
     1741 ccatgtgcgg gagtgttgcc gctgacagtc cggtcagccg gagaaccggc tcgccggtga
     1801 ggctgatttg catctgttta attttctgtt ccagcgatga tttcacctgc tgcatgacag
     1861 ccttttccgg tgcgacgtag ccctgatggc cggtggtgtt ggcgagcgga ttttcctgta
     1921 ccagaatgct cagatgcatt gcccggacaa gcgcctcaca ggtttcattc agggcgtgtt
     1981 ccagatcatg ctctgcatac agactcagaa ggtgatgatg tgccttccgg taggcggtgg
     2041 ccgtgctgtc acacccccct ttcaggcgtt cacgttcgaa attcagcacc acggccagat
     2101 tgtcatattc ctgtaccagc tcccggcgtg ccacgcgctc aatgtggcgc tgtttcagct
     2161 cgtcgctcag gacaccaccg gctgcacgaa aggccgtgcg ccagtcgtca gcgtcgtttc
     2221 cgtcggcctg cgccagcgca tttttttcct gctctgcccg ttcaatggcc gtgatggtct
     2281 catccatcag gcgggcgttc tcaagatggg cggctctggc cttttccagt tgtgccagcg
     2341 cgggttgcag atattcaggg atggtgttgt cagacatttt ccggctcctc gtcacttcag
     2401 gttaagaaaa ttgtgacgta caccggacaa caacacgacg cattgcagat gtgccagccc
     2461 tgacacagga gactcatcct cagaccggca agccaggaaa aggtcgcagg aaaaaccggc
     2521 ttactgtttg tttttttata ttttactgtt cacctctgtt caccttaata aaaaagataa
     2581 gtaatacagt aagttaaagg gtgaacaatc gcagtaatga ctgttcaccg tctgttcacc
     2641 actgttcacc cgctcatgga ctttttgtgc tgtttactac tgtttatttt tattaattca
     2701 ctgaaataaa taagaaaaaa caatttgtat ttcactataa aaaattccag gtccttcaga
     2761 accctttgag acccttccag tccagatgga taaaaaacac acaatcattg taaggcagcc
     2821 tgaacaaatc cccctgttgc gtctgctgaa aatattcaca aaataaagcg ctacccgaag
     2881 ccggacggac ttatccggtg ctgtatggac attaacgagg tagcccgatg caagctgttt
     2941 tttcttcccc gtctcccgcc cctgtgacgc cactgatgcc gctgccggac atcacgcagg
     3001 agcgtttttt acgtctgccg gaagtgatgc acctgtgcgg cctgtcacgc tcgaccatct
     3061 acgaactcat ccgtaagggg gaatttccgc cgcaggtgag tcttggcggt aaaaatgtgg
     3121 cctggctgca ctctgaaatc accgcatgga tggccgggcg cattgccgga cgcaaacggg
     3181 ggtacgacgc atgatgatgc ccgctctgca aaaagtccct ttttctggct tgcctttttc
     3241 cggcatttgc ggatatagtt tttccgctgc cgcaaaatcg gcagccgggc gtaggaaccc
     3301 gtgtaactcg aaggcgacat atgacgcgcc atgcgtcttt ttttgtgtcg caatcaacgc
     3361 cacagagcgc cagattatgg tgtggcgtgt ggtttgccgt gcaggtatga tcctgttcgc
     3421 aatcgcatgt tatgccactg agtcaatggt agctcaggcg gggcagcctc cgggctggcc
     3481 ggtattcttc gaggccggta ttcctacccc cgtctgggct atcgccatcg agcgtaggaa
     3541 ctccggcgat agcagttatt tgctactcga aggagactgc cttatggcta caacccttac
     3601 cccttcacac cctgaatttg tctttgtgtt tgcggctgtc cgtcgcgcag accgtcatcc
     3661 ccgtatctgc atgcttcgca ccgtcgccgg tgatgaacgc agcgcccgcc gttcccttgt
     3721 ccgtgactat gtgctctccc ttgctgcccg tctgccggtg gtggaggtgt cccgtgcgta
     3781 ataaaaaagc ccctcagacc gtctcagcgc gtcatgacgc ccgtgaacac ctcagcattg
     3841 aggcttacca taagctcaac cgcgccagcg cagtatcccg gtttgttggg ggtgatttga
     3901 ttcaccgtga actctccggc ctgcatcagc tctacattcc gcatattttc agctacctga
     3961 atgaagatat tgattttgtg ctgaatgagc tgaaagccaa aggcctgtgc cgcgattttc
     4021 tcgcccagca gaaagaccgg ggagacagga cacatgtttg attttcccca gcccggtgag
     4081 atttaccgtt ctgccggttt tcccgatgtg gccgtggtcg gcattctgga agacggtatt
     4141 ccgtgggaaa tgccgtaccg ctgtccggaa attgtctgga acccgtaccg ccgaaaattc
     4201 agtatccttg tgcgtatcct cactgacggg cgcaccacag acatcccgct ggggcgtttt
     4261 ctgcgggaat ttacctgtga ccgtcctgac ctgttcaaac gcagccccgt aaaccggcat
     4321 gcggtactga aagaaatggc cggagacccg gaattacaga aatggcggga gaaatatctg
     4381 gatatttacc cgcaggacac tgttccggcc agccgggcgg caccggtggc gcgggaatgg
     4441 cgggaaattt cccgcacgga gcctgacccg gaaatcaccc cggataacag ttaccgcaat
     4501 tatctgtaat taaaaaacga cacccgaaaa attaaatgtg cgtattcgcg cagggatacg
     4561 cacgtcttca ggagacgcag atatgcctta tcagttaatg caaccggcac ggaatgcagt
     4621 catctgtcac agggaggaaa acaaatgaaa acccccttac cgcccgtctt acgcgcagcc
     4681 ctttaccgtc gcgctgtcgc ctgtgcctgg ctgaccgtgt gcgaacgtca gcaccgctac
     4741 ccgcatctca cccttgaatc actggaggcg gccatcgccg ctgagctgga aggcttttat
     4801 ctgcgccagc acggtgagga aaaagggcgt cagatagcct gtaccctgct ggaagattta
     4861 atggaatccg gccccctgaa ggccgcgccg tcgctgtcct ttctcgggct ggttgtgatg
     4921 gatgaactct gtgcccgtca cataaaagcg ccggtactgc actgaaggag aacaacacca
     4981 tgaaaatgaa cgtaaccgct accgtcagcc atgcgctcgg ccactggccg cgtattctcc
     5041 cggcgctggg gattcaggtg ctgaagaacc gtcatcagcc ctgtccggtc tgtggcggga
     5101 gtgaccgttt ccgttttgat gacagggagg ggcgcggcac ctggtactgc aatcagtgtg
     5161 gtgccggtga cggcctgaaa ctggttgaaa aggtgtttgg tgtttccccg tccgatgcgg
     5221 ccgcaaaggt ggctgccgtg accggcagcc tgcccccggc tgacccggca gtgacggctg
     5281 ccgccggtgc tgaaacagac gctgcccgga agaacgccgc cgcactggca caaaccctga
     5341 tggcgaaaac ccgtcccgga accggtaacg cctacctgac ccgcaagggc tttcccggcc
     5401 gggaatgccg gatgctgacc ggcacacaca gagccggtgg cgtgagctgg cgtgccggtg
     5461 accttgtggt gccactgtat gacgacagcg gcgaactggt taaccttcag ttaatcagtg
     5521 ctgacggccg taagcgcacc ctgaaaggcg gacaggtcag gggcacctgt cacacccttg
     5581 aaggacagaa tcaggccgga aaacgtctgt ggatagcgga gggatacgcg accgcactta
     5641 ccgtgcatca cctgaccggt gaaacggtga tggtggcgct ttcttccgtg aacctccttt
     5701 ctctggccag ccttgcccgg cagaagcatc cggcctgtca gattgtcctt gccgcagacc
     5761 gtgacctcag cggtgacggc cagaaaaaag ccgccgcagc cgcagatgcg tgtgaaggtg
     5821 ttgttgccct gccgccggtc ttcggtgact ggaatgatgc cttcacgcag tacggcgggg
     5881 aagccacccg taaggccatt tatgatgcca tccggccacc ggctgaaagc ccgttcgaca
     5941 ccatgagcga agcggagttt tccgccatga gtaccagcga aaaggccatg cgtatctatg
     6001 agcattacgg cgaggcgctc gcggtcgatg ccaacggcca gcttctgtcc cgctatgaaa
     6061 atggtgtctg gaaggtgctg ccgccacagg actttgcccg ggatgtggcc gggctgtttc
     6121 agcgtctgcg cgcgccgttc tcctccggga aggtggcctc cgtggtggac accctgaagc
     6181 tgattattcc gcagcaggaa gccccctccc gccgcctgat tggctttcgt aacggcgtgc
     6241 tcgacacgca gaacggcacg ttccacccgc acagtccgtc acactggatg cgcaccctgt
     6301 gcgatgtgga tttcaccccg ccggtggaag gggaaacgct ggaaaaccac gcccccgcgt
     6361 tctggcgctg gcttgaccgt gccgccggtg gccgtgcgga aaaacgcgac gtgattctgg
     6421 ccgcactgtt tatggtgctg gcaaaccgct acgactggca gctctttctg gaggtgaccg
     6481 gtcccggcgg cagcggcaaa agtatcatgg ccgaaatcgc caccctgctg gccggggagg
     6541 ataacgccac atcggccacc attgagacgc tggaatcccc gcgtgaacgt gccgcgttaa
     6601 ctggcttctc actgatacgc ctgccggacc aggaaaaatg gagcggcgac ggtgccggac
     6661 tcaaggccat caccggcggc gatgcggtgt ccgtggaccc gaaataccgg gatgcgtact
     6721 ccacgcatat cccggcggtg attctggccg tgaacaataa cccgatgcgc ttcaccgacc
     6781 gcagcggcgg cgtgtcacgc cggcgggtga ttattcactt cccggaacag atagccccgc
     6841 aggagcgcga cccgcagctt aaggacaaaa tcacccgcga gctggcggtc atcgtgcgtc
     6901 acctgatgca gaaattcagc gacccgatgc tcgcccggtc actgcttcag tcccagcaga
     6961 actcagacga ggcactgaac atcaaacggg atgccgaccc gacgtttgat tttatcggct
     7021 atctggaaac cctgccgcag accagcggca tgtatatggg gaacgccagt atcatcccgc
     7081 gtaattaccg taaatacctc tatcacgcct atctggccta catggaggca aacggctacc
     7141 ggaatgtact cagtctgaaa atgttcgggc tggggctgcc ggtgatgctg aaggaatacg
     7201 ggctgaatta cgagaagcgc cataccaaac aggggataca gaccaacctg acgctgaaag
     7261 aggaaagcta cggcgactgg ctgccaaaat gtgacgaccc tgcaacaacc tgaccctcat
     7321 gactcatctg accggcatct gccggtcttt tttcatccct gaatcccccg aaggtgaaca
     7381 atccactgtt cacccttcac cgtatattca cccgttatca cactgaaatt aaaagagaaa
     7441 aacgaaaggt gaacagtgtg aacaatcaaa tcaaaaaaaa cttttttctt cctgagtgat
     7501 ttcagtgcgg aggattaatc accggtatga gtcacatcgg cagaatgccg gaggtgaaga
     7561 atcgaatgtt cacccttcac ccattattca ccacctatca aactgaaata aaaggagaaa
     7621 acagaaaggt gaacagtgtg aacagttctt ccgaaaaaaa atttttttcc tgagttaacg
     7681 tgcgtgaaga tacgtgtaac aattaatcga tagatattat atgtataagg atgtgtataa
     7741 atgaattaga atagaaaaat ataaacgatt tgaatcaagt ggttatatgg caattttgac
     7801 tcctgtgatc ttccgccaaa atgcctctcc tgaactctcc cgacgtcaac aacactcctt
     7861 cggaggcgac atgtcagaca aagacttaac ccagccccca gcaggcgagt ttattatgtt
     7921 tgccagcggt gacggaagag tgcgtgttga atgtcgcttt gagtcagaca caatctggtt
     7981 atcccaggcg gcaatggctg aactgtacga taaagatgtt cgcactatta acgaacattt
     8041 aatcaatata ttttccgaag gtgaacttgt tcaaaactca accatccgga aattccggat
     8101 agttcgccag gaaggaaaac gtcaggtttc cagagagata gaccattaca acctcgaagc
     8161 cattctcgct gtcggctatc gcgttcgctc gcctcgaggc acgcaattcc gccagtgggc
     8221 cacccagacg cttcaggaat acctgatcaa aggttttgtg atggacgatg agcggctgaa
     8281 gaatccgcct gtcggttcat cggttgtgcc cgactatttt gatgagatgc ttgaacgcat
     8341 ccgcgatatt cgcgccagcg agcgtcgggt atatttgcgg atgcgggaga tctttgcttt
     8401 agctgccgac tatcagccat cgcttaaaga aaccacgcaa ttttttcaaa ccatccagaa
     8461 taaactgcat tttgcctgta ccgggcatac cgctgcggaa ctcatccaca aacgagcaga
     8521 tgccagccag ccgcacatgg gcttgaccag ttataaaggt gaagaggtac gtaagggcga
     8581 cgtgacggtg gcaaaaaatt atctcaccca ggatgaggtc agcgaactta atcgcgtggt
     8641 taatatgtgg ctggactttg ccgaggatca ggcccgtcgt cgtcagcaag tctttttgcg
     8701 cgactggcag gataagctgg atcagttcct gcaatttaac gaccgagagg ttctacaggg
     8761 cgctggtaaa atcagcaaga aaatggccga tgaaaaagcg caggcggagt atgttcagtt
     8821 tgccgaacaa caacggcgct taaaagaagc cgaaggcgag aaggatatcg ctggtttgct
     8881 acagtggaat aaagagtcga aaaagtagcc cggaatcccc ccaaacgcca tacgcaaaac
     8941 catttggggg tacttatggg gttactcatt tatcaaattc aaaatataaa cattaaatca
     9001 ataaattaac catcaaatgc gactcctgtg atcgccaaaa tgcctcttca gtgatcccca
     9061 tcctgcttat ttctgagcaa cctgaccctg ctgatgtatc tggtagtatg acagagtgat
     9121 tcgtttttct tgccgagtgt acgaaaacgt cttccttgcc accgactatc aaccgtcact
     9181 caaaaaaacc tctctttttt tcaagctatc aagaataagt tgcattttat ctgcaccgga
     9241 caccagccat ccatatatgg cttttacctg ctacaaagat gaagaagtgc ataaatgtga
     9301 tgttaaaaca atc
//
LOCUS       DAZGJT010000142         9302 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZGJT010000142 DAZGJT010000000
VERSION     DAZGJT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9302
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.145"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            331..1671
                     /gene="dcuB"
                     /locus_tag="ACHKFK_004266"
     CDS             331..1671
                     /gene="dcuB"
                     /locus_tag="ACHKFK_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO9054483.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            1749..3395
                     /gene="fumB"
                     /locus_tag="ACHKFK_004267"
     CDS             1749..3395
                     /gene="fumB"
                     /locus_tag="ACHKFK_004267"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO9054484.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDIQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPEELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            3518..4168
                     /gene="yjdF"
                     /locus_tag="ACHKFK_004268"
     CDS             3518..4168
                     /gene="yjdF"
                     /locus_tag="ACHKFK_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO9054485.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDPWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITRAPEAITP"
     gene            complement(4165..5520)
                     /gene="melA"
                     /locus_tag="ACHKFK_004269"
     CDS             complement(4165..5520)
                     /gene="melA"
                     /locus_tag="ACHKFK_004269"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO9054486.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTILHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTVEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKAGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5779..6711
                     /gene="melR"
                     /locus_tag="ACHKFK_004270"
     CDS             5779..6711
                     /gene="melR"
                     /locus_tag="ACHKFK_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO9054487.1"
                     /translation="MFQERESSMNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPH
                     IMPTSHWHGQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMA
                     IFNLPMHLFLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQL
                     AIDEIGLMLKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTL
                     NDVAEHVKLNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGF
                     RSSSRFYSTFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            6910..9177
                     /gene="adiA"
                     /locus_tag="ACHKFK_004271"
     CDS             6910..9177
                     /gene="adiA"
                     /locus_tag="ACHKFK_004271"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO9054488.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANIGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         2308 a         2498 c         2301 g         2195 t
ORIGIN      
        1 attaatctat aaacctaatg atacctgccc tgagaacaaa aaacagaccg ataaatatca
       61 ataagataac agcaaacaaa acattaacat ctgcgctgta caaactataa acccatcgcc
      121 agagagtctt tctctctgaa aaagccgctt atcacagtgc ataaatttgc cgctgcttta
      181 atcagccaat attcactgtg aggtatttgc tcaagccggt aacgaccaaa cggatattta
      241 gtcaggctct gaaaacagtt catacaaaac agaacgtgac tgtgatctat tcagcaaaaa
      301 tttaaatagg attatcgcga gggttcacac atgttattta ctatccaact tatcataata
      361 ctgatatgtc tgttttatgg tgccagaaag gggggtatcg cgctgggttt attaggcggt
      421 atcggtctgg tcattctggt cttcgtcttc caccttcagc caggtaaacc accggttgat
      481 gtcatgctgg ttatcattgc ggtggtggcg gcatcggcga ccttgcaagc ttcgggcggt
      541 cttgatgtca tgctgcaaat tgccgagaag ctgctgcgtc gcaacccgaa atatgtctca
      601 attgtcgcgc cgtttgtgac ctgtacgctg accattcttt gcggtacggg tcatgtggtt
      661 tacaccattc tgccgatcat ctacgacgtt gctattaaga acaacatccg tccggaacgt
      721 ccgatggcgg caagttctat cggtgcacag atggggatta tcgccagtcc ggtgtcggtt
      781 gcggtcgtat ctttggttgc gatgctgggt aatgtcacct ttgatggtcg ccatcttgag
      841 ttcctcgacc tgctggcaat caccattcca tcgacgttaa tcggtatcct ggcgatcggt
      901 atcttcagct ggttccgcgg taaagatctg gataaagacg aagagttcca aaaattcatc
      961 tccgtaccgg aaaaccgtga gtatgtttac ggtgataccg cgacgctgct ggataaaaaa
     1021 ctgccgaaaa gcaactggct ggcaatgtgg atcttcctcg gggcaatcgc tgtagtcgcc
     1081 cttcttggtg ctgattcgga cctgcgtcca tccttcggcg gcaaaccgct gtcgatggta
     1141 ctggttattc agatgtttat gctgctgacc ggggcactga ttattatcct gaccaaaacc
     1201 aatcccgcgt ctatctcaaa aaacgaagtc ttccgttccg gtatgatcgc catcgtggcg
     1261 gtgtacggta tcgcatggat ggctgaaacc atgttcggtg cgcatatgtc tgaaattcag
     1321 ggcgtactgg gtgaaatggt gaaagagtat ccgtgggcct atgccattgt tctgctgctg
     1381 gtttccaagt ttgtaaactc tcaggctgcg gcgctggcgg cgattgttcc ggtcgcgctg
     1441 gcgatcggcg ttgatccggc atacatcgtg gcttcagcac cggcttgcta cggttattac
     1501 atcctgccga cttatccgag cgatctggca gcgattcagt ttgaccgttc cggcaccacc
     1561 cacatcggtc gcttcgtcat caaccacagc tttattctgc cggggttgat tggtgtgagc
     1621 gtatcgtgcg tcttcggctg gatcttcgcc gcgatgtacg ggttcttata aatgcacttt
     1681 gcgtgccgcc cgtgactacg cggcacgcca ttttcgaata acaaatacag agttacaggc
     1741 tggaagctat gtcaaacaaa ccctttatct accaggcacc tttcccgatg gggaaagaca
     1801 ataccgaata ctatctactc acttccgatt acgttagcgt tgccgacttc gacggcgaaa
     1861 ccatcctgaa agtggaacca gaagccctga ccctgctggc gcagcaagcc tttcacgatg
     1921 cttctttcat gctccgcccg gcacaccaga aacaggttgc ggctattctt cacgatccag
     1981 aggccagcga aaacgacaag tacgtggcgc tgcaattctt aagaaactcc gaaatcgccg
     2041 ccaaaggcgt gctgccgacc tgccaggata ccggcaccgc gatcatcgtc ggtaaaaaag
     2101 gtcagcgcgt gtggaccggt ggcggtgatg aagaagcgct gtcgaaaggc gtctataaca
     2161 cctatatcga agataacctg cgctattcac agaacgcgcc gctggacatg tataaagagg
     2221 tcaacaccgg cacgaacctg cctgcgcaaa tcgacctgta cgcggtagat ggcgatgagt
     2281 acaaattcct ctgtgtcgct aaaggcggcg gctctgccaa caaaacgtat ctctaccagg
     2341 aaaccaaagc cctgctgacg cccggcaaac tgaaaaactt cctcgtcgag aaaatgcgta
     2401 ccctcggtac tgcagcctgc ccgccgtacc atatcgcgtt tgtgattggc ggtacgtctg
     2461 cggaaaccaa cctgaaaacc gtcaagttag caagcgctca ctattacgat gaactgccga
     2521 cggaagggaa cgaacatggt caggcgttcc gcgatatcca gctggaacag gaactgctgg
     2581 aagaggccca gaaactcggt cttggcgcgc agtttggcgg taaatacttc gcgcacgaca
     2641 tccgcgttat ccgtctgcca cgtcacggcg catcctgccc ggtaggcatg ggcgtctcct
     2701 gctccgctga ccgtaacatt aaagcgaaaa tcaaccgcga agggatctgg atcgaaaaac
     2761 tggaacacaa tccaggccag tacattccag aagaactgcg tcaggcaggt gaaggcgaag
     2821 cagtgaaagt cgaccttaac cgcccgatga aagagatcct tgcccagctt tcgcaatacc
     2881 cggtatccac ccgcttgtcg ctcaccggca ccattatcgt gggtcgcgat attgcacacg
     2941 ccaagctgaa agagctgatt gacgccggta aagaactgcc gcagtacatc aaagatcacc
     3001 cgatctacta cgcgggtccg gcgaaaacgc ctgccggtta tccatcaggt tcacttggcc
     3061 caaccaccgc aggtcgtatg gactcctacg tggatctgct gcaatcccac ggcggcagca
     3121 tgatcatgct ggcgaaaggt aaccgcagcc agcaggttac cgacgcgtgc cataaacacg
     3181 gcggcttcta cctcggtagc atcggcggtc cggcggcggt actggcgcag cagagcatca
     3241 agcatctgga atgcgtcgct tatccggagc tgggtatgga agctatctgg aaaatcgaag
     3301 tagaagattt cccggcgttt atcctggtcg atgacaaagg taacgacttc ttccagcaga
     3361 tcgtcaacaa acagtgcgcg aactgcacta agtaacctct tcggcccagc gcctggcagc
     3421 atgctgccag gtgatccccc tgggccacct cttttgcgat tgtaatttca cgcttactgg
     3481 tcaatagtca gtattttccc tgacttgcga actcaccatg acccgcacac tcaagccgtt
     3541 aattcttaac accggcgcac tggcgctaac gttaatcctg atttataccg gcatttcggc
     3601 ccatgacaaa ctcacctggc tgatggaagt gacaccggtg attattgtcg tgccgctact
     3661 gcttgccacc gctaaacgtt acccgttaac gccgctgctc tatacgctca ttttctttca
     3721 cgccatcatc ctgatggtcg gcgggcaata cacctacgcg aaagtccccg ttggttttga
     3781 ggtgcaggaa tggctggggt tgagccgtaa tccgtatgac aaactggggc attttttcca
     3841 ggggctggtg cctgcactgg tggcacgaga gatccttgta cgcgggatgt acgttcgcgg
     3901 acgtaagatg gtggcgtttc tggtctgctg cgtggcgctg gcgataagcg cgatgtatga
     3961 gttaatcgag tggtgggcag cgctggcaat gggccagggc gcagatgatt ttctcggtac
     4021 tcaaggcgac ccgtgggata cgcaatccga tatgttctgc gcactactgg gcgcattaac
     4081 gacggtgata ttcctcgccc gctttcattg ccgccagcta cggcgctttg gcttaatcac
     4141 cagagcgccg gaagcgatta ccccttaacg gtgcaaccag cctggcagcc agtcgccgtg
     4201 ggcggcaatc aggtcgtcaa caagagcata tatttcgtca atgcccagca cggcggcagt
     4261 atgcgggtcc atcatcgcgg cgtggtaaac acggtcgcga ttttccgtaa gaatagcttc
     4321 tgtcagcagc gtctgtacgt tgatgttggt ttgcatcagg gcggccagat gcgaaggtag
     4381 cgtaccggct ttggtcggct gaatgccatt agcatcaacc agacaggcca cttccacgca
     4441 acatccttgt ggcaggttat caatcaagcc atcgttacgg acattgccat aaatcacact
     4501 cggctcgcca gtccagatag cgttcatgat tgtgctggca tattcccgtg acggtttaat
     4561 atcaatccgg gaggcgtttt tatactcctc cagctcttta tgccagttcg ccagctgctc
     4621 gacgcagcgt ttcgggtact catccagcgg tactttataa cgctcaatca aatcctcacg
     4681 acctggctta ataaaccacg gtgtgtactc agcaaaatgt tctgacgatt ctgtgacgaa
     4741 gtagcccagc tttttgaaca tttcgtaacg cacaatattc tggcagcgag tattaccatg
     4801 aatatttggc ttcggtgcct gccctgcttc ataagccgcc agcaattcag gatagagatt
     4861 cacgtagcta ccgtctgcgg ttttacgctc cagttccagg taaaacgcca tatggttgat
     4921 acctgcgcaa cggtaacgca gcgtagccgg atcgatattg aggtcacgcg ccagctcttc
     4981 caccgttccc tgcaccgaat ggcacagccc tacctgcttg atatgcggat agcgtgcata
     5041 catcgcccag gtattcatcg ccattgggtt aacgtagttg agcatggtgg catcggggca
     5101 gacttccgtc atgtcctcgc aaatctgcca caggtgcaga atggtacgta gcgcacgcat
     5161 aatgccgccc ggccccagcg tatcggcaat ggtttgttcc agaccatgcc gcttacagac
     5221 ctcgaaatca gtcaccgtgc aaggttcata accgccaatc tgaaatgcca ccaccacaaa
     5281 atcagcatcc tgtaaggctt ctttctgttg ggtgtggcag gtgattttgc cgctggcccc
     5341 tgctgaatcc atcagcttac gcaccacaat gtgcgactct tccaggcggg tgggatcgat
     5401 atccatcagg gcaatatgcg ccgttttcag cgcctctcga tggaacacat caccaagaat
     5461 atttttaacg aaaatcgtcg aaccagcgcc gataaatgta attttgggtg cagacatcat
     5521 gcagatctcc tggcttgctt gaataacttc atcatggcag gcgatatcgc agaaaacctc
     5581 cggtttcccg gttgagcatt cccgaaaact cagatcctaa gtggcacatc aatagatctg
     5641 agtttatggg aataaattac ctgaaaagct gaataatcga cccaaatctt ctcatccgct
     5701 ctcctcgcac ctccagggag aggaataaaa ccgtgcgcct gttcgcagtc atcctccctc
     5761 actcctgcca taattctgat attccaggaa agagagtcat ccatgaatac agatacgttt
     5821 atgtgcagca gcgacgaaaa gcagacccgt agcccactgt cgctgtattc agaataccag
     5881 cggatggaaa ttgagtttcg cgcaccgcat atcatgccca ccagccactg gcatggtcag
     5941 gtcgaagtga atgtgccttt cgatggcgat gtggaatacc tgatcaacaa tgaaaaagtg
     6001 aatatcaatc agggtcatat cacgctgttc tgggcctgta caccgcacca actaacagat
     6061 accggaacct gtcagagcat ggcgattttt aatctgccga tgcatctgtt tctctcctgg
     6121 ccgctggata aagacctgat taaccacgtc actcacggca tggtgatcaa atcactggcg
     6181 acacagcaac ttagcccgtt tgaagtgcgc cgctggcagc aggaattaaa cagtccgaac
     6241 gagcaaattc gccagctcgc cattgatgaa attggcctga tgctcaagcg atttagcctc
     6301 tctggctggg aaccgattct ggtcaataaa acctcgcgca cacacaaaaa cagcgtctcg
     6361 cgccatgcgc aattttatgt cagccagatg ctgggcttta ttgccgaaaa ctatgatcag
     6421 gcgctgaccc tcaacgatgt ggctgagcac gtcaaactta acgccaacta tgcaatgggg
     6481 atattccagc gggtcatgca attgacgatg aaacagtaca ttaccgcgat gcgcatcaac
     6541 cacgttcgcg cgttactgag cgataccgat aaaagtattc tcgatattgc cctgacggca
     6601 ggctttcgtt cgagtagccg tttttacagc acgttcggca aatatgtcgg catgtcgccg
     6661 caacaatacc gcaaacttag ccaacagcgc cgccagacgt ttcccggcta aagacgatat
     6721 cagtatcagc caaaaaaata gtttagccgt cgataataag cagtgaaaat aatttaaaat
     6781 aatcacataa cgtattgttt ataaaaataa atatttcaaa aatgtttgtt tttcacgcgc
     6841 tttacagccc gaaaaggccg gaagatactt gcccgcaacg aagattcctt cataaccggg
     6901 taagcaatga tgaaagtatt aattgttgaa agcgagtttc tccatcaaga cacctgggtc
     6961 ggtaacgccg ttgagcgtct ggcagatgct ttaagccagc aaaatgttac cgtgattaaa
     7021 tccacctcct ttgatgatgg ttttgccatt ctctcttcaa acgaagccat tgactgcctg
     7081 atgttcagct atcaaatgga acatccggac gaacatcaaa acgtcagaca attgatcggt
     7141 aagcttcatg agcgccaaca aaacgtgccg gtcttcctgt tgggcgatcg ggaaaaagcc
     7201 ctcgccgcaa tggaccggga tctgctggag cttgtcgatg aattcgcctg gattctggaa
     7261 gatactgccg actttatcgc cggacgcgcc gttgccgcga tgacccgcta ccgccagcag
     7321 ctgttgccgc cactgttcag cgcgctgatg aaatatagcg acatccatga atattcctgg
     7381 gcagcgccag gccaccaggg cggcgttggt tttactaaaa cacccgccgg acgtttctac
     7441 catgactact atggtgaaaa tctgttccgc accgacatgg gcatcgaacg cacttccctc
     7501 ggttctttgc ttgaccatac tggcgcattt ggcgaaagcg aaaaatatgc cgcacgcgta
     7561 tttggtgccg atcgctcctg gtcggtagtc gtcggtactt ccggctctaa ccgcaccatc
     7621 atgcaggctt gcatgaccga taacgatgta gtggtcgttg accgtaactg ccataaatcc
     7681 atcgaacaag gcttgatgct gacaggcgcg aaaccggtct acatggtgcc aagccgcaac
     7741 cgctacggca ttatcgggcc aatatatccg caggaaatgc aacctgaaac cttgcagaag
     7801 aaaatcagtg aaagcccgct gaccaaagac aaagccgggc aaaaaccgtc ttactgcgtg
     7861 gtgaccaact gcacctatga cggcgtgtgt tataacgcta aagaagcaca ggatctgctg
     7921 gaaaaaacct ccgatcgtct gcactttgac gaagcctggt acggctatgc acgtttcaac
     7981 ccgatctatg ccgatcacta tgccatgcgc ggcgaacctg gcgatcacaa cggtcctacc
     8041 gttttcgcca cccactccac ccacaaactg ctgaatgcgc tgtcacaggc ctcttatatt
     8101 catgtacgtg aaggtcgtgg ggcgattaac ttctcccgct tcaaccaggc ctacatgatg
     8161 catgccacca cctccccgct gtatgccatc tgtgcatcca acgacgtggc agtgtcgatg
     8221 atggacggca acagcggcct gtcactgacc caggaagtga tcgacgaagc ggttgatttc
     8281 cgtcaggcga tggcgcggct atataaagag ttcaccgctg acggtagctg gttcttcaaa
     8341 ccgtggaaca aagaagtcgt caccgaccca caaaccggca aaacctatga ctttgctgac
     8401 gcaccaacca aactgctgac caccgttcag gactgctggg tcatgcatcc gggcgaaagc
     8461 tggcacggct tcaaagatat tccggataac tggagtatgc tcgacccaat taaagtcagc
     8521 atccttgctc cgggaatggg tgaagatggt gaactggaag aaaccggtgt tccggcggcg
     8581 ctggtcacag cctggcttgg tcgccacggc attgtgccta cccgtaccac tgacttccag
     8641 attatgttcc tgttctctat gggcgtgacc cgtgggaaat gggggactct ggttaacacc
     8701 ctttgctcct tcaaacgcca ctatgatgcc aacacaccgc tggcgcaggt gatgccggaa
     8761 cttgttgaac aatatcctga cacttacgcg aacataggga ttcacgatct gggtgacacc
     8821 atgtttgcct ggctgaaaga aaacaaccct ggcgcacggt tgaatgaagc ctattccggc
     8881 ctaccggtgg cggaaatcac cccgcgtgaa gcgtacaacg cgattgtcga caacaatgtc
     8941 gaactggtat ccattgaaaa tctgccagga cgtatcgcgg caaactcagt tatcccgtat
     9001 ccgccaggaa tcccgatgct gctgtctggt gaaaacttcg gcgataaaaa cagtccgcaa
     9061 gtaagttatt tacgctcgct gcaatcctgg gaccatcatt tccctggatt tgaacacgaa
     9121 acagaaggga cagaaattat tgacggtatt taccacgtta tgtgtgtgaa agcgtaacca
     9181 ctattccgct gaaggcgtaa ttgttaaata acattacgcc gcctggcttg aggcctttta
     9241 agtatggcaa cgttttcata aaaattgctg caaacaaaaa atgtcatgct ttttgcgcgg
     9301 tc
//
LOCUS       DAZGJT010000143         9211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZGJT010000143 DAZGJT010000000
VERSION     DAZGJT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.146"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..256
                     /locus_tag="ACHKFK_004272"
     CDS             <1..256
                     /locus_tag="ACHKFK_004272"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HFO9054489.1"
                     /translation="VKPPFLIMHGSKDTLVSPEQSAKLFRMLKKNGDNAEYVLVEGAE
                     HGDKTWYQPIIINRVVEWFTKNLGAPIKTAPQQQNPNANL"
     gene            complement(293..3802)
                     /locus_tag="ACHKFK_004273"
                     /pseudo
     CDS             complement(293..3802)
                     /locus_tag="ACHKFK_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773871.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter beta domain-containing
                     protein"
     gene            complement(4052..4753)
                     /locus_tag="ACHKFK_004274"
     CDS             complement(4052..4753)
                     /locus_tag="ACHKFK_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO9054490.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRKNGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNASELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTSL
                     YQNMRANALTDTELRRKAADELTCMTARINRGEAIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGVMK"
     gene            complement(4750..5769)
                     /locus_tag="ACHKFK_004275"
     CDS             complement(4750..5769)
                     /locus_tag="ACHKFK_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO9054491.1"
                     /translation="MTGSEMAKAGLREQSRLSGANRNALIAEGIMANTAEIFNFPVPD
                     EAQKEPRVADLDDGYTRIANELLEAVMLAGLTQYQLLVFLAVMRKTYGFNKKLDWVSN
                     EQLSELTGILPHKCSAAKSVLVKRGIFIQSGRNIGINNVVSEWSTLPESGKKNKVYLK
                     EVNLPELGKKSLPKSGKDVYPNQVNTKDKLTKDNIKPFSSENSGESSDQSENDLPVVK
                     PDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHR
                     DMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWI
                     YGVDL"
     gene            complement(5766..6304)
                     /locus_tag="ACHKFK_004276"
                     /pseudo
     CDS             complement(5766..6304)
                     /locus_tag="ACHKFK_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182892.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT domain-containing protein"
     gene            complement(6374..6604)
                     /locus_tag="ACHKFK_004277"
     CDS             complement(6374..6604)
                     /locus_tag="ACHKFK_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO9054492.1"
                     /translation="MNPAIKTAINIVGSQKKLGAACEVSQQAVYKWLHNKAKVSPEHV
                     GSIVTATGGVVKAYQIRPDLPELFPHTEKNAA"
     gene            6709..7398
                     /locus_tag="ACHKFK_004278"
     CDS             6709..7398
                     /locus_tag="ACHKFK_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO9054493.1"
                     /translation="MKTTLSERLKEARLARGLTQKALGDLVGVSQAAIQKIETGKANQ
                     TTKIVEIANALGVRAEWLSSGVGNMSDSIVQPIQSTVSHSKYFKIDVLDIEVSAGPGV
                     INREFVEVLRSVEYSFDDARHMFDGRKAENIRIINVRGDSMSGTIEPGDLLFVDITVK
                     SFDGDGIYAFLYDDTAHVKRLQMMKDKLLVISDNKSYSPWDPIEKDEMNRVFIFGKVI
                     GSMPQTYRKHG"
     gene            7994..8200
                     /locus_tag="ACHKFK_004279"
     CDS             7994..8200
                     /locus_tag="ACHKFK_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor protein"
                     /protein_id="HFO9054494.1"
                     /translation="MVHQHYGTQTVNRGAVMPGMLVKHKDGTWTASANLRGRLYLHRG
                     IERTYTRDLLVEVFLDGRGNGLNH"
     gene            8276..8572
                     /locus_tag="ACHKFK_004280"
     CDS             8276..8572
                     /locus_tag="ACHKFK_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9054495.1"
                     /translation="MNAYYIQDRLEALSWARHYQQIAREEKESELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            8578..>9211
                     /gene="bet"
                     /locus_tag="ACHKFK_004281"
     CDS             8578..>9211
                     /gene="bet"
                     /locus_tag="ACHKFK_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9054496.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNSIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWIDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYEKDEAERIVENTAYTAERQPERDITPINDETMQEINTLLIA"
BASE COUNT         2444 a         2252 c         1999 g         2516 t
ORIGIN      
        1 agtaaaaccc ccatttctta ttatgcatgg tagcaaagac actctggttt cacctgagca
       61 aagcgccaaa ctattcagga tgttgaagaa gaacggcgat aacgctgagt acgtgctggt
      121 agaaggggcc gagcatggcg ataagacatg gtatcagcca attattataa acagagtcgt
      181 tgagtggttt actaaaaacc tgggagcgcc tataaaaaca gctccccaac aacaaaaccc
      241 aaacgctaac ctgtaaaaag agggagggct aagccctccc cattcaattt tgttaactat
      301 ccttttcagg tagttttaca acataagtcc ttattgtttt ctcatatgta tttttgctat
      361 tcgttatttt ggccttaatc cagtgataac cactttcata ggtgctgaat tccgaagctg
      421 cattacctgt ccagtgtaac gttactgtag caggctccat tttgacccgt tgagagtctg
      481 gactatcttc actaccagca gaaaactcaa ccgtaccaga taatgggttt ccaaaatgat
      541 cgacaaatcg agcataaata cctactggag agccatcgtt tgtctcataa ccaggatctt
      601 ttgttagagt caatactcca tttgcgtcat cagcgtacag ggagaatgat ttcaccgcgc
      661 taacatcact gccaagttcg tttaaggtcg ccgttaattt atacgaacca accgttcgac
      721 catgtacgct taccgtagcc tgaccgttct catcagtcgt tactgtggtt ttatcaacca
      781 ccaacgcacc atatttagac ggcccagatg tcttaacatt caatgctcga ccacttaatg
      841 cttcgcctga cttacttttc aacagtaacg taaataccag attgttggta tcgctcacta
      901 cagccgaaga tgcagatgat gtgatctcca acacagcccc cttcacatcc gtcacggcat
      961 caatattctg gcttgcggtt atgcgtttcc catccagaac atactcggcc tgaattgtgt
     1021 actggcctga tttcgatgca gtgaactgcg ttgttgcctg tccatgagca tccagttgca
     1081 gattactact ggtcaatgat gctcccgtcg atggtgtaat cgttaaatcc acctcgcctg
     1141 taaacgcatt gttattcgca tcaaccaact gaatgtttac tgttgcgttt tcactgccat
     1201 cggccacaat ctcttgtttt gatacactca tagtaagttc tgcagaagca acatcgggca
     1261 caaaagttaa cttaacgctg ccagattcca cgctatggga accgtcagtc acccgtgcag
     1321 tcaccgtgta ctcaccagct ttcaccgttg taagcggaac agaaacatgc cctgttgaat
     1381 cagtcacgat atttgttggt acagataatc cttcagatga ggtgatgagc tgaatctttt
     1441 gcccattgac cgacgcattc gtatttgtca gctgcacatc taataccgct gcatccttac
     1501 cattagcagg aatattggaa attaaactac tgctttcagg cgtcagtgac agtgaagctc
     1561 cggtcatatt tgatgcaaag gtcactgtta actcagtaga catctgagaa ccagcatgag
     1621 ctgtaatcac gtaagacccc ggagtggagc ttattaacgc aaaaatagca ttaccatttt
     1681 cgtcagttga aacagcatgt tcaccaccaa cttgagttat ccctgctggt aaagacaatg
     1741 tgacagcata accaggaaca acattgccga aacggtccgc aaggcttaca gttaccatat
     1801 ttctctgttt tccatcagcc aatgcattat tttggctggc ttcaaactga gagaatgtaa
     1861 cctgctgccg gtcctcaata aaggtgattt ctttcttaac acctgagaaa tcatgactat
     1921 cagatttaac accgatagta atcttaccag ctcgttttga agttactgtc gcgctataga
     1981 caccgtcttt ttccgttaca gtcccaaact cgactccttc tgcctggtta agagcataaa
     2041 agctcagagt gttatcacca gtgattgcat ttccttgtga atccttcact gccagttgta
     2101 gattgatatt gtaaccaact acctgttctg ctggtgcagc ggttaaaaca gcactgacct
     2161 cagaatcagg ttctgttgct gctgtttgct taatactgag agtaaatgtt tttccctgaa
     2221 cttttgcagt tacacgtacc gtcccagcct gcgaaccagc agccagaaca gagcgatata
     2281 caccagcaga gatttcctct accgcgccta atgacggagc agttaccgtt tcacgctgtc
     2341 gagcactatt gctatccgca gtaaattcca cagacatttc aatgttatca gccagtccgg
     2401 ttaatgcctt accgttactg tctttcaggc ttaatactat tggataagtg gattgaccat
     2461 cagcagagat cgtcggagac gaatccccat ccagcgtaaa agatgaatcc gctgtcgaga
     2521 caccactatt attaacgctg atattcataa cggcatagtt ggaaacattg cctctgttat
     2581 cctggactgt ggctccaaca ttccacgaat ttacaccttc gcttttataa gccggtaaag
     2641 tgatctgcca tgatgtgcca tttccactgt acagacttga taccaccact agcattgttg
     2701 attgtcagtg ttacagggat gattgattcc ccctccccct gaacagactc tggcaaactg
     2761 attttcagtg catgcttctt cttgtactcc agaacaatgt tgttatttcg ttcaacaaaa
     2821 tcataacgcc gattctggac ttctcgcata actgcaacat tatcgctgct aagctgttca
     2881 gccagggaaa cgcccgggcg ataattaaac tcaacaccga aggttgtatc gtgaacgttg
     2941 ctctgtcctt gcttatgctg tgcagaaaac ttaatcagag gaactggtgt ataagaaatt
     3001 cccccagtaa ctgcgtaagg gttttcctga aggttatcgc ttccaaataa cccgacattt
     3061 ttaccataat atttttcaaa ctgaatagat gcccctaatt gcggataagc aggtagccat
     3121 ccttcagcag aaaaatccca gccatttgca ggtctttcca gataatcatc aatatcccga
     3181 ctgttcttcc agtcagatag accaaaatag gtatttacac caagcctgaa ataatccctc
     3241 cagtactcaa ccccaaaacc tgcacgagag tgactgcgac ttaaatcgta atcatagaaa
     3301 acattcgcac ccaacattgc gttatcagga gtgaaatgac gaatccccaa accaatattg
     3361 gtctgatttc ggtcatcagt acgatgtagt gatgtctgac tgaataacac ataatcctga
     3421 gtatccagcc atggatataa aaagtcgaat gatgaatcct tcaaggaaaa agaatcatcg
     3481 acattaagct tgatgcgcgc attgccatat tgttgcaacc agtcgacgac ctcttttgtc
     3541 gcctgagttg ataaagtatt tacagcaaaa ctacttgcat tattattcgc taggctctga
     3601 cctgcacttg ctgcaaatga ggccacttta tttgcatgct catcgctggc ataagtttgc
     3661 gtaacatctt tattaccaga tgatgcaaaa ctgtttgctg ggatcaaaga aagagaaact
     3721 ggagataata tctgggtaac aattaccgac catgtaatcg cgccagatgc agttttttta
     3781 actttttatt cacagtggtc atagttcaat caacgttatg aatataatga aaaaaataac
     3841 tggtatgaca agaggcgcga aatatacaga tcgttacaaa aaaatcaact caaaaataaa
     3901 ataaaatctc tgcataaact ttatggctaa atgatttcac aacaatggaa gagacacttt
     3961 gttactcacg caatcaatca tttcgggcat aacaactagt cctagattat attcgctagg
     4021 tatactttat ttttaagcta atactttagc tctatttcat cactcctttc agcccgaact
     4081 tagctttgat ttctgcgatc ttcgccagcg cctgaacacg atttagaggt ctaccgccca
     4141 tgacaggaag ttgttttact ggttcaggta tcgcctcacc acggttaatt cgcgcggtca
     4201 tacaagtcag ttcatcggca gccttgcgcc gtaattccgt atcagtaagc gcattggccc
     4261 gcatattctg gtacaggcta gtaaccagcc agtagtgcgc gtttgatttc cacggataag
     4321 actctgcatc cggatacagg ccacgcttcc ggcaatactc gtaaaccata tcaaccagct
     4381 cgctggcgtt tggcagtccg gcaataacgg atgcttcttc acggcaccat gcaacaaact
     4441 gcccgggtga tggcaggaat ggtcgattct gccgacgggc tacgcgcatt cctgcgttaa
     4501 cctgttccat tgtggtgatc ccgtttttcc ggaaagccag cacccactgg cggcgaattt
     4561 cgttcagttc gttctggtcc cggttagcca ggctcgccgg gaaagttgcc agtaactggc
     4621 tgaacacacc attgatgatc tgcgctacct gttgtacctg cggcttttcg tcgtactgtt
     4681 ccggcatatt gttggcgatc cgacgcatct gctcacggtc aaagttaacc atctgtgcgg
     4741 cgatgttttt catagatcca ccccgtaaat ccagtcagtg tttgtcaggt cgagttttgg
     4801 tttgctagct gtcacgccag cctgttgctt gttacggttg atttcgagtt gggtccactt
     4861 gtcgcggagt ttggccggac ttagcacgtt accggaccag aagttgtcct ggcatgccca
     4921 gcggaacagt acacacatgt cgcggtggtt acgtccgtca cgttcacgca tcaggcggat
     4981 atcgttagcc caccctgcaa aattcggttt tctggctgat ggcgcgatgg tcttcaccat
     5041 gtcaaacatc cactctgcgg cggtcaggtc ttctgctgtc ccccacttgc tgccgctctg
     5101 aattgcagca tccggtttca ccacaggaag atcgttttct gactggtcag aggattcgcc
     5161 agaattctcg gacgaaaaag gttttatatt gtcttttgtt agtttgtctt ttgtgtttac
     5221 ctgattcggg taaacgtctt tacctgattt gggtaaactt ttcttaccta attcaggtaa
     5281 atttacctct ttcaggtaaa ctttattttt cttacctgat tcgggtaatg ttgaccattc
     5341 actgaccaca ttattaatgc cgatattccg cccgctctga ataaaaatcc cacgctttac
     5401 cagaacactt tttgcagcag aacacttgtg cggcaatatc ccggtcaact cggaaagttg
     5461 ctcgttgctc acccaatcca gttttttatt aaagccatat gttttgcgca tgacagccag
     5521 gaagaccaga agctggtact gtgttaatcc ggccagcatc acagcttcca gcaactcatt
     5581 tgcaatgcgc gtataaccat catcgagatc tgccacgcgc ggctcctttt gtgcctcatc
     5641 cggcactgga aaattgaata tctcagcagt gtttgccata attccttccg caatgagtgc
     5701 gttacgattt gcacctgaaa gtcggctctg ttcccgcaga ccggctttcg ccatttctga
     5761 acctgtcata ttgcccccag catggtggta accatcgcca ttaatggacc agccagatcc
     5821 gggtccacac gaaacatcga cacaatgcct tcactcatct ccttcagttt ctggtggcgt
     5881 ggtgcgttga gaatgaccgc ctgctttgcc tcactgagtt ccttttccat ttcagccagc
     5941 cgagccatga agctatcctg ctcaaccagg tggccgcgat attccagcgg tagtaccgcc
     6001 agaattgccg gggtcagttc acgcacgtta tttcggtatt tttcagaatc gaatttgtta
     6061 tcgaggaagc ggaacagctt ctggcgtgca cggctgacat catcagggaa atcgatggtg
     6121 ccgccgccct gctcccgata ctcattcaca atgagtgcgg caacaacatc ctgattatct
     6181 gcagccgacc aggcgcgaac ggcatcacgg atttttcgtg gcctggcgcc tgttttgttt
     6241 gagaacgatt tatcaccgca gtcgggctaa atccgctagt ctgttggtat gtaagtggtt
     6301 gcataattga ctcctttagt ttgaattgac tgttaagttg attgcttatt gttaaagagc
     6361 gtgaaatgga aatttaagct gcgttctttt cggtgtgtgg aaacaactcc ggaagatccg
     6421 ggcgaatctg gtatgccttc actactccac cagtagccgt aacaatgctg ccgacatgtt
     6481 caggggatac ctttgctttg ttgtgaagcc acttatagac ggcctgctgt gaaacttcgc
     6541 aagcagcgcc cagtttcttt tgtgaaccaa cgatattgat cgctgttttg atagctgggt
     6601 tcataacaac ctccgtggtt aatttgaatc aagattaaaa ctatggttgt ttttaatcaa
     6661 caaccatttt cgtttgatgg aataaaacct tggttgtaca tttggactat gaaaacaaca
     6721 ctctcagaaa gacttaaaga agccagatta gcgcgaggcc ttacacaaaa ggcgcttggg
     6781 gatttggtcg gggttagcca ggctgctatt cagaaaatcg aaacagggaa agctaatcaa
     6841 acaactaaaa tcgtggagat cgcgaacgct ttgggtgtgc gcgcagaatg gttatcttct
     6901 ggcgttggaa atatgtcaga cagtatagtg caaccaatac aatcaactgt cagccattcc
     6961 aaatacttca agattgacgt tcttgatata gaagtcagtg ctgggccggg agtcatcaac
     7021 cgtgagtttg tagaagttct acgctcggtt gagtactcgt ttgacgatgc tcgtcacatg
     7081 ttcgatggta ggaaggcgga aaatatccgc atcattaacg tgcgtggtga cagcatgtca
     7141 ggaacgatcg aaccaggtga tctgctgttc gttgatatca cagttaaatc tttcgacggt
     7201 gatggtatct atgcgtttct gtacgacgac acagcccatg taaagcgcct gcaaatgatg
     7261 aaggataagc tgctggtcat ctctgataac aaaagctact caccgtggga cccgatcgag
     7321 aaagacgaga tgaaccgggt gttcatcttc ggtaaggtta ttgggagcat gccgcagaca
     7381 tataggaagc atgggtagta ccaattaaaa attatcaact gggcattgtg ctcattcagt
     7441 aaagaactaa ttcctatctt tgctctaggt agtaatatta agccaccgca ataatatctt
     7501 tacctaacgg cgtaagaatc ccggtcaccg tgccgggttt tcttttgccc tcccctcatc
     7561 acacacaccg ttaaaaaaac caccataacc tcgcttcagt tatcgctatg cgattcaagt
     7621 cacaaaataa atccatccta aatacaacca gttatatcta aaacaaccaa taaaacaact
     7681 tttgttgttg atgataaaac aactatagtt ttaaataggt tcatcgcaac aacacaacga
     7741 tacggcaact acctgattca ccgttgcgat gaccgcttag atccgcagtt tgaatttcag
     7801 caggcttcgg ggagtgcgag gggtgaaacg gacgcgtgaa cgtcggtgtg accagctgaa
     7861 atcaactcaa catttcatac cttagtcgct tcaacgaggc ggcttagtta tgacaaccgg
     7921 cggccatcca ccgcctgaat acgcgcagaa gtctctatat gttcagcagc ccagcttacg
     7981 ggcaggagtt tttatggttc atcaacatta cggaacgcag accgttaatc gcggcgcggt
     8041 catgccagga atgctggtca aacacaaaga tggtacctgg actgcatcag ctaatttacg
     8101 cggacggctt tatctgcatc gcggcatcga gcgcacttat acccgtgatt tgctcgtgga
     8161 agtttttctc gacggacgcg gtaacggcct gaatcactaa tcccctttcc tgttttccta
     8221 atcagcctgg catttcgcgg gcgatatttt cacagccatt ttcaggagtt cagccatgaa
     8281 cgcttattac attcaggatc gtcttgaggc tctgagctgg gcacgtcact accagcagat
     8341 tgcccgtgaa gagaaagagt cagaactggc agacgacatg gaaaaaggtc ttccacagca
     8401 cctgtttgaa tcactctgca tcgatcattt gcaacgctgc ggggccagca aaaaagccat
     8461 tacccgtgcg tttgatgacg atgttgagtt tcaggagcgc atggcagaac acatccggta
     8521 catggttgaa accattgctc accaccaggt tgatattgat tcagaggtat aaaacggatg
     8581 agtacagcac tcgcaacgct ggcagggaag ctggctgaac gtgtcggcat ggattctgtc
     8641 gacccacagg aactgatcac cactcttcgc cagacggcat ttaaaggtga tgccagcgat
     8701 gcgcagttca tcgcattgtt gatcgtcgcc aaccagtacg gccttaatcc gtggacgaaa
     8761 gaaatttacg ccttccctga taagcagaac agcatcgttc cggtggtggg cgttgatggc
     8821 tggtcccgca tcatcaatga aaaccagcag tttgatggca tggactttga gcaggacaat
     8881 gaatcctgta catgccggat ttaccgcaag gaccgcaatc atccgatctg cgttaccgag
     8941 tggatcgatg aatgtcgccg cgaaccattc aaaacccgcg aaggcagaga aatcacggga
     9001 ccgtggcagt cgcatcccaa acggatgtta cggcataaag ccatgattca gtgtgcccgt
     9061 ctggccttcg gatttgctgg tatctatgaa aaggatgaag ccgagcgcat tgtcgaaaat
     9121 accgcataca ctgcagaacg tcagccggaa cgcgacatca ctccgattaa cgatgaaacc
     9181 atgcaggaga ttaacactct gctgatcgcc c
//
LOCUS       DAZGJT010000144         9167 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZGJT010000144 DAZGJT010000000
VERSION     DAZGJT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.147"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(88..414)
                     /locus_tag="ACHKFK_004282"
     CDS             complement(88..414)
                     /locus_tag="ACHKFK_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO9054497.1"
                     /translation="MKRTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVAL
                     KTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVERAKPGEYVQGING
                     KWIRKF"
     gene            complement(422..607)
                     /locus_tag="ACHKFK_004283"
     CDS             complement(422..607)
                     /locus_tag="ACHKFK_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO9054498.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(604..3243)
                     /locus_tag="ACHKFK_004284"
     CDS             complement(604..3243)
                     /locus_tag="ACHKFK_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO9054499.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLSVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVAAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPALVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVVKPRLILDKPVVWGRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEDKQ"
     gene            3451..4440
                     /gene="ldhA"
                     /locus_tag="ACHKFK_004285"
     CDS             3451..4440
                     /gene="ldhA"
                     /locus_tag="ACHKFK_004285"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO9054500.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4551..4973
                     /gene="hslJ"
                     /locus_tag="ACHKFK_004286"
     CDS             4551..4973
                     /gene="hslJ"
                     /locus_tag="ACHKFK_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO9054501.1"
                     /translation="MKKVAALVALSLLMAGCVSSDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4970..5236)
                     /locus_tag="ACHKFK_004287"
     CDS             complement(4970..5236)
                     /locus_tag="ACHKFK_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO9054502.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5510..9034
                     /gene="nifJ"
                     /locus_tag="ACHKFK_004288"
     CDS             5510..9034
                     /gene="nifJ"
                     /locus_tag="ACHKFK_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO9054503.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAASVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILELMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTSEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRNVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPVRSAYLISQANFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSAAEVWSRLPQEVQAVLNQKKACFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNSEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2281 a         2405 c         2248 g         2233 t
ORIGIN      
        1 ttcttataat taaaagagca aaatgtttcc ggaaaataga ttaatcgttt tctcctgtag
       61 cgttatcccg atattgcggt tttgctatta aaattttcgt atccatttac cgtttatccc
      121 ttgtacatat tccccgggtt tggcccgttc tacgagcttt tgcccggcga gtttcgcgat
      181 atcatccacc gacacattat tttgctttgc cagttgttga tagctcgctt tacgttcggc
      241 attaatgtcg gttactaatt tctcagtttc agcatctgtt ttcagcgcaa ccagataacc
      301 ataaaatgtt tcccctaccc gcccctgagt tctggcttca tccagagtca atgccattac
      361 attgctgctt accaagccaa caagaaatgc acaaagaagt aatgttcttt tcatcatcac
      421 ctcaaaaaag atcgctacgg gtctcaagca gctcttcgac atctttgtct gccttgatga
      481 tgatctcatg ctcaatttta acgttcatat tgatagtgat cggttcctta ggtgcagcga
      541 cttcaatgcg aggcgtacag ccaaccagca taaaagatga cgtcaacgca gccagtaaaa
      601 ttttcattgt ttatcctcac actcttttcc ttgcggacag tcatttcccg gcagacgcgc
      661 gttctgctcc agccatgcct ggagattatc gccaaagcgt aaactgcgcc acagcgtaaa
      721 aatattctct tcatgatggt aattaagatt taccgtaccg cttttaccat caacgcgact
      781 ggtacctgta atattggcct gcatggttaa taaaccgaga ttatctaaat taatttttgt
      841 cgatgaacgg ctaatttcca tatagcgcaa ccagttaatt gctgaacccg cggtcatatt
      901 gtctttcacc accgcatctg cggtgtcttt atccagccgc aaggtcatcg gcccgctatt
      961 tgccagccag ccgtctttca ctatccattt ttcgttgttt agccataacg gcaatgcacc
     1021 actaaatgcc ccggacatgg cgaattgttt cggattgacg gcgctaacca gttcgctgga
     1081 ggaaagatta ttcagccgca acagcgccgg gtcatgttgc ggcatacgta attgtttcat
     1141 cagcacgtta ccgcctaaca catcgacgct aacatcagtc aacagcaatg gttcttcttc
     1201 agtccacgga taacgacctt gcaaatcagc cgtaatattt ttcgctgtca ccagattaat
     1261 cacttcggca attcgcaacg taacgggacc gcgagtcccc agatgccagg ctccatcggc
     1321 aaaacggaaa ggcaggacaa aatcgacacc gttaacctga ttatctggca tccaggcact
     1381 accgcctttc aacacgccgt gccctcccgc gcggaatcct tgttcgggcg ctgctgaaaa
     1441 tgcgacctga gcatacagtt caccatcgcg taagttcatc ttccagtcgg gtggcaccaa
     1501 cggctggaat acagtcagtg attgtttagg ccaccaggcg ttaccgcgca gacgaatacc
     1561 gtcccagcga ccatttaccc gaaccgggcc aatctcacca gcatgtaaat cgcctttaaa
     1621 gaggaaatac gtcggatcgc gcccatcgac actaaatttt aaggttgatg gcggtaacac
     1681 actgccgcca gtgaacagcg tttgcccggc gtccagtgac agagcgccgc tgaaggaggg
     1741 atgctgtgcg tcacgtcccc agacgacggg cttgtcgaga attaatctcg gctttactac
     1801 cgtcatcgta ccgtattgta actggtcgaa accggtggag agatcggtca gcgtaatcgt
     1861 gctgtcatgc cactcacctt ttcctgcgac atcccaggtg gcattcatcg gtgtaaaact
     1921 cccttttccc cagtagcgcc actgccagcg gccagcgtca gggagaaaat cattcgccag
     1981 cccatccata tgcagcacga aatcgcccag ttcattttca tgcgcctgca aaatggcctg
     2041 caaacgtccg tcaacaccac gttgggtgac ttttacaccc gctaaaggcc agcggatttc
     2101 atcgatgtcc agcgaatcaa tgactcttcc cttcgaacgt aataacgcgc caggctcaaa
     2161 ggtcagcgtt gggtcagaca gacttccact tagctgcgca ggtaaacggg catataaaat
     2221 gaggtccgcc tgtttcgctt caccggtcag ctgcagaggc agctggctgt tatccatgct
     2281 taattttcct gggccaaaat ttaacaccgc gttaccttta cccgcttgcc cctgggtcag
     2341 cacactcagt cgcccgctga ccagagcgtt ctcgagccct gcctgccagt tgtcgacttt
     2401 aacacccagt cggccactca aaggaaaacc tgcatacggc cagctccagc gaccatcgct
     2461 tacggtcaat tgttgacgag taatttgcca cggcaaatcg agcaacggat cgccgttatc
     2521 ccgtgccagc acaatcaatt gcccgctatt ttcctgccag tccagctcgg catccaccag
     2581 tgcaggctcc tgcggtaagt ttaacgtcgc agtagcatga ccacttaccg gaagtccatc
     2641 cggcacgagc ggcatagtaa attcccccac cagttttacc ggcggctgat tttcaaacgc
     2701 ggcgacatcc agttcgctga ctgtaagttg ttgccctttc agctggcctt gaaatttaac
     2761 tttttcgccc tgataacgca gttgctggat atcggaggtt aatgcgagag agagttttcc
     2821 ctgccattcc tgccagggag aaaaaatcag tttatcgata ttgatccagg tgttaggcag
     2881 catggactgc cactgcgcga gggtttttgg agcggctggc gattgctccg tctgcggcaa
     2941 tttcgccaga caagcagaat caagttctac cgtgccgacg ctcaataacc agcggctggg
     3001 atgtgaaagg ctggcgttgg tgatatgcgc aagctgacaa tctcccacca gataacggag
     3061 atcggggatg attaaacctt tacgcgtaat gcgtgggctt tcatctaatg caatacgtgt
     3121 cccgagcggt agccagatgc ccgccagcgt gggaacccac agcccgagcg tcatcagcag
     3181 cgtcaacggc acaagaataa tcagtaataa cagcgcgaga acggctttat atttacccag
     3241 catgggtagt taatatcctg atttagcgaa aaattaagca ttcaatacgg gtattgtggc
     3301 atgtttaacc gttcagttga aggttgcgcc tacactaagc atagttgttg atgaattttt
     3361 caatatcgcc atatctttca attatatttg aaattttgta aaatattttt agtagcttaa
     3421 atgtgattca acatcactgg agaaagtctt atgaaactcg ccgtttatag cacaaaacag
     3481 tacgacaaga agtacctgca acaggtgaac gagtcctttg gctttgagct ggaatttttt
     3541 gactttctgc tgacggaaaa aaccgctaaa actgccaatg gctgcgaagc ggtatgtatt
     3601 ttcgtaaacg atgacggcag ccgcccggtg ctggaagagc tgaaaaagca cggcgttaaa
     3661 tatatcgccc tgcgctgtgc cggtttcaat aacgtcgacc ttgacgcggc aaaagaactg
     3721 gggctcaaag tagtccgtgt tccagcctat gatccagagg ccgttgctga acacgccatc
     3781 ggtatgatga tgacgctgaa ccgccgtatt caccgcgcgt atcagcgtac ccgtgacgct
     3841 aacttctctc tggaaggtct gaccggcttt actatgtatg gcaaaacggc aggcgttatc
     3901 ggtaccggta aaatcggtgt ggcgatgctg cgcattctga aaggttttgg tatgcgtctg
     3961 ctggcgttcg atccgtatcc aagtgcggcg gcgctggaac tcggtgtgga gtatgtcgat
     4021 ctgccaaccc tgttctctga atcagacgtt atctctctgc actgcccgct gacaccggaa
     4081 aactaccatc tgttgaacga agccgccttc gatcagatga aaaatggcgt gatgatcgtc
     4141 aataccagtc gcggtgcatt gattgattct caggcggcaa ttgaagcgct gaaaaatcag
     4201 aaaattggtt cgttgggtat ggacgtgtat gagaacgaac gcgatctgtt ctttgaagat
     4261 aaatccaacg acgtgatcca ggatgacgta ttccgtcgct tgtctgcctg ccacaacgtg
     4321 ttgtttaccg ggcaccaggc attcctgaca gcagaagctc tgaccagtat ttctcagact
     4381 acgctgcaaa acttaagcaa tctggaaaaa ggcgaaacct gcccgaacga actggtttaa
     4441 tcttgccgct ctcctgcatt ccaggggagc ttattcagat aatccccaaa gacctttcat
     4501 cctctattct taaaatagtc ctgaatcaga aacagtaatt gagaaccaca atgaagaaag
     4561 tagccgcgct tgttgcgcta agcctgctga tggcgggatg tgtaagtagt gacaaaattg
     4621 ctgtaacgcc agaacagcta cagcatcatc gctttgtgct ggaaagcgta aacggtaagc
     4681 ccgtgaccag cgataaaaat ccgccagaaa tcagctttgg tgaaaagatg atgatttccg
     4741 gcagcatgtg taaccgcttt agcggtgaag gcaaactgtc taatggtgaa ctgaccgcca
     4801 aagggctggc aatgacccgt atgatgtgcg ctaacccgca gcttaatgaa ctcgataaca
     4861 ccattagcga aatgctgaaa gaaggtgcac aagtggatct gaccgcgaac cagttaacgc
     4921 tggcgaccgc gaaacagaca ttaacttata agctggcgga tttaatgaat taatagctgc
     4981 cacagctccc ggcggcaagt gactgttcgc tacagcgttt gccgttgggt aatgcacaca
     5041 tcccaatcgc cgtaccatcc agttgacggg caacagaaag cgaaccgccg atcattgcac
     5101 aatttgcttc tccactactg gacatcgacg cttttaaacc tggcgctacg tgcgccgcag
     5161 tcgcctgctg aacaggttca ctactacacg ccgacaacaa taaagcggca caccctaccc
     5221 aaaacgctgc tcgcatcttt tcttcctctg atcttcaagc caaacgacac cgccataaat
     5281 aataggcagc acagagggcg gcgtcgagag ctgtcctgcg cgttgccccg catttttact
     5341 tttttatggc tatttttttg ccctctgttt gatcaaaaca ttcattacgc tgatgtgggg
     5401 gacacaaaag cgaaaatgca gaagaaagcc atttgctaaa attgaacgat tactactggg
     5461 cgcgcagcaa tttcgtgcgc ccctcatttg cgcaatgtaa gggtgtcata tgattactat
     5521 tgacggtaat ggcgcggttg cttcggtggc gtttcgcacc agtgaagtta tcgccatcta
     5581 ccctattacc cccagttcca cgatggcaga acaggctgat gcatgggccg gaaacggctt
     5641 gaaaaacgtt tggggagaca caccacgcgt ggttgaaatg cagtcggaag cgggtgctat
     5701 cgctaccgtg catggtgctt tgcaaactgg tgccctctca acatcgttta cgtcatcgca
     5761 gggtttgctg ctgatgatcc caacgctgta caaactggca ggcgaactga cgccgtttgt
     5821 cctgcatgtt gcggcacgta ccgttgccac acatgcactc tctatttttg gcgatcattc
     5881 cgacgttatg gcggtgcgcc aaacgggttg cgcgatgttg tgtgcagcaa gcgtccagga
     5941 agcgcaagac tttgctctca tttcgcacat cgcgacgctg aaaagccgcg tgccatttat
     6001 tcatttcttt gatggtttcc gcacatctca cgaaatcaat aaaattgtcc cgctggccga
     6061 tgacacgatt cttgaactga tgccgcaggc agaaattgat gctcatcgcg cccgggcact
     6121 caacccggaa catccggtga tccgcggtac gtccgccaat cctgacactt atttccagtc
     6181 tcgcgaagcc accaacccat ggtacaacgc ggtctatgac catgttgaac aggcgatgaa
     6241 tgatttcgct gccgcgacag gtcgtcagta tcagccgttt gagtattacg ggcatccgca
     6301 agcggaacgg gtgattatcc tgatgggctc tgccattggc accagtgaag aagtggttga
     6361 tgaattgctg acccgtggcg aaaaagtcgg cgtattgaaa gttcgcctgt accgcccctt
     6421 ctccgccaaa cacttgctgc aagctctgcc gggatccgtg cgcaacgtgg cggtactgga
     6481 cagaaccaaa gaacccggtg cccaggcaga accgctctat ctggatgtga tgaccgcact
     6541 ggcagaagcc tttaataatg gcgagcgcga aaccctgccc cgtgtcattg gtgggcgcta
     6601 tggtctttca tccaaagaat ttggcccgga ctgtgtactg gcggtatttg ccgagctcaa
     6661 cgcggctaaa ccgaaagcgc gctttacggt tggtatttac gatgatgtaa ccaatctgtc
     6721 actgccgttg ccggaaaaca ccctaccaaa ctcggcgaaa ctggaagcct tgttttatgg
     6781 ccttggtagt gatggcagcg tttccgcgac caaaaacaat atcaagatta tcggaaattc
     6841 cacgccgtgg tacgcacagg gctattttgt ttacgactcc aaaaaggcgg gcggcctgac
     6901 ggtttctcac cttcgcgtga gcgaacagcc ggttcgttcc gcttatctca tttcccaggc
     6961 taattttgtt ggctgccacc agttgcagtt tatcgataaa tatcagatgg ctgagcgttt
     7021 aaaacctggc ggcattttcc tgctcaacac gccgtacagc gcagctgaag tgtggtcgcg
     7081 cttgccgcag gaagttcagg ccgtgctaaa ccagaaaaaa gcgtgcttct atgtgattaa
     7141 cgcggcgaaa atcgcccgcg aatgtggcct ggcggcccgt attaataccg tcatgcagat
     7201 ggcttttttc catctgacgc agattctgcc tggcgatagt gccctcgcag aattgcaggg
     7261 tgcgattgcc aaaagttaca gtagcaaagg ccaggatctg gtggaacgca actggcaggc
     7321 tctggcgcta gcacgtgaat ccgtagaaga agttccactg cagccagtta atccgcatag
     7381 cgccaatcga ccgccagtgg tttccgatgc cgctcctgat ttcgtgaaaa ccgtaaccgc
     7441 tgcgatgctc gccgggcttg gcgacgccct ccccgtctcg gcgctgccgc cagacggcac
     7501 ctggccgatg ggcaccacgc gctgggaaaa acgcaatatc gccgaagaga tccccatctg
     7561 gaaagaggaa ctctgtaccc aatgtaacca ctgtgtcgcc gcttgcccac actcagctat
     7621 tcgcgcaaaa gtggtaccgc ctgaagcgat ggaaaacgcc cctgccagcc tgcattcgct
     7681 ggatgtgaaa tcgcgtgata tgcgcgggca gaaatatgtc ttgcaggtgg ctccggaaga
     7741 ttgcaccggt tgtaacctgt gcgtcgaagt ttgcccggcg aaagaccgtc agaatccaga
     7801 gattaaagcc atcaatatga tgtcgcgcct ggaacatgtc gaagaagaga aaatcaatta
     7861 cgatttcttc ctcaacctgc cggaaatcga ccgtagcaaa ctggaacgta ttgatattcg
     7921 tacatcgcag ctgattacac cgctgtttga atattccggt gcttgctccg gttgtggcga
     7981 gacgccgtat attaaattgc tgactcagct ctatggcgac cggatgttga tcgctaatgc
     8041 cactggctgt tcttcaatat acggcggtaa cctgccttca acaccatata ccaccgatgc
     8101 caatggtcgt ggaccggcat gggcgaactc gctgtttgaa gataactccg aatttggcct
     8161 tggtttccgc ctgacggtcg atcaacaccg tgtccgcgtg ctgcgtctgc tcgatcaatt
     8221 tgccgataaa atcccggcgg aattactgac tgcgttgaaa tcagacgcca cgccagaggt
     8281 tcgtcgtgaa caggttgcag ctttacgcca gcaactcaac gatgtagccg aagcacatga
     8341 actgctgcgt gacgcagatg cactggtgga aaaatcaatc tggctgattg gtggcgatgg
     8401 ctgggcttac gatatcggct ttggcggtct ggatcatgta ttgagtttga cggaaaacgt
     8461 caacattctg gtgctggata cgcaatgcta ttccaacacc ggtggtcagg catcgaaagc
     8521 gacaccgctg ggtgcagtaa ctaagtttgg cgagcatggc aaacgtaaag cgcgtaaaga
     8581 tcttggcgtc agtatgatga tgtacggtca tgtttatgtg gcgcagattt ctctcggcgc
     8641 gcagctgaac cagacggtga aagcgattca ggaagcggaa gcgtatccgg ggccatcgct
     8701 gatcatcgct tatagcccat gtgaagagca tggttacgat ctggcactca gccacgatca
     8761 gatgcgccaa ctcactgcta ccggcttctg gccgttatat cgcttcgatc ctcgtcgtgc
     8821 cgatgaaggc aaactaccgc tggcgctgga ttcacgcccg ccgtcagaag cactggaaga
     8881 aacgttactt cacgagcagc gtttccgtcg tctgaattcg cagcagccag aagtggcgga
     8941 acagttatgg aaagatgctg cagctgattt gcaaaaacgc tatgacttcc tggcacaaat
     9001 ggccggaaaa gcggaaaaaa gcaacaccga ttaaatgctc tggataagga tcatccaatt
     9061 ctaaaaaaaa gcccggacga ctgttcgggc ttgtcttctt atatgttgga aaatcagtag
     9121 caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcat
//
LOCUS       DAZGJT010000145         9129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZGJT010000145 DAZGJT010000000
VERSION     DAZGJT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.148"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            123..665
                     /gene="ecpR"
                     /locus_tag="ACHKFK_004289"
                     /gene_synonym="matA"
     CDS             123..665
                     /gene="ecpR"
                     /locus_tag="ACHKFK_004289"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO9054504.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKLYKLVQ"
     gene            739..1326
                     /gene="ecpA"
                     /locus_tag="ACHKFK_004290"
                     /gene_synonym="matB"
     CDS             739..1326
                     /gene="ecpA"
                     /locus_tag="ACHKFK_004290"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO9054505.1"
                     /translation="MKKKVLAIALVTVFTGTGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1383..2099
                     /locus_tag="ACHKFK_004291"
                     /pseudo
     CDS             1383..2099
                     /locus_tag="ACHKFK_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2125..4650
                     /gene="ecpC"
                     /locus_tag="ACHKFK_004292"
                     /gene_synonym="yagX"
     CDS             2125..4650
                     /gene="ecpC"
                     /locus_tag="ACHKFK_004292"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO9054506.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASATDISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMTLANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQIWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTFSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQSVYSGTFGSLGLRAGIQRYNNGDSSANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIDASGRTDGNAGVIFDTGLENDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4640..6283
                     /gene="ecpD"
                     /locus_tag="ACHKFK_004293"
     CDS             4640..6283
                     /gene="ecpD"
                     /locus_tag="ACHKFK_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO9054507.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGRRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6252..6962
                     /gene="ecpE"
                     /locus_tag="ACHKFK_004294"
     CDS             6252..6962
                     /gene="ecpE"
                     /locus_tag="ACHKFK_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO9054508.1"
                     /translation="MFRRRGVTLTKALLTVVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNLTKRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDQVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7275..7604
                     /gene="ykgJ"
                     /locus_tag="ACHKFK_004295"
     CDS             7275..7604
                     /gene="ykgJ"
                     /locus_tag="ACHKFK_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO9054509.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGEVNEACNRARA
                     KYGLTPL"
     gene            complement(7616..7782)
                     /locus_tag="ACHKFK_004296"
                     /pseudo
     CDS             complement(7616..7782)
                     /locus_tag="ACHKFK_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885624.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(7853..8467)
                     /gene="yagU"
                     /locus_tag="ACHKFK_004297"
     CDS             complement(7853..8467)
                     /gene="yagU"
                     /locus_tag="ACHKFK_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFO9054510.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNSAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            complement(8682..8867)
                     /locus_tag="ACHKFK_004298"
     CDS             complement(8682..8867)
                     /locus_tag="ACHKFK_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001468276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054511.1"
                     /translation="MASGLICHGGFEPHAWGVANLMDVAVLVVAYLLVWLLVNRENHY
                     ARGIPQDVQVLHKKAHH"
     gene            8916..9075
                     /locus_tag="ACHKFK_004299"
                     /pseudo
     CDS             8916..9075
                     /locus_tag="ACHKFK_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         2464 a         2309 c         2257 g         2099 t
ORIGIN      
        1 gctacgtatc ttatttactt ccgatttact aaagaaactg aatgtacctg taaaaattac
       61 aggtttggaa agtagtgaca tggcaaaatg attacagcag ggactatgag gttaaaaacc
      121 atatggaatg tcaaaaccgt tctgataaat acatctggtc tccccatgac gcctacttct
      181 ataaaggact atctgaactg attgtggata tcgacagatt aatttatcta tcgctggaga
      241 aaatcagaaa agatttcgtg tttatcaatc tcaatacgga ttctttaact gagtttataa
      301 accgtgataa tgagtggtta tccgcggtaa aggggaaaca ggtcgtattg attgcggcca
      361 gaaagtcaga agccttagca aattattggt attacaacag caatattagg ggcgtggtat
      421 acgctggact gagtcgtgat attagaaaag aactggccta tgtgattaat ggcaggttcc
      481 tgagaaaaga tattaagaaa gataaaatca ctgaccggga aatggaaatt atccgcatga
      541 cggctcaggg aatgctgcct aaatcgattg ccagaattga aaattgtagt gtgaagacag
      601 tgtataccca tcggcggaat gcagaggcca agctgtactc aaaattatat aagttggttc
      661 agtaaactcc aggcaagtta gttttaaaaa tgactcaccg ggacttcatg tcctcaattc
      721 aactcgggaa gaaaagcaat gaaaaaaaag gttctggcaa tagctctggt aacggtgttt
      781 accggtacag gtgtagcgca ggctgctgac gtaacagctc aggctgtagc gacctggtca
      841 gcaacagcca aaaaagacac caccagtaag ctggttgtga cgccactcgg tagcctggcg
      901 ttccagtatg ccgaaggcat taaaggtttt aactcacaaa aaggtctatt tgacgtggct
      961 atcgagggtg actcaacggc taccgccttt aaactgacct cacgccttat caccaacacc
     1021 ttaacccagt tggatacctc aggttccaca ctgaatgtgg gcgtggatta taacggcgcg
     1081 gcagtcgaaa aaactggcga taccgtgatg atcgataccg ccaacggcgt actgggcggc
     1141 aaccttagcc cactggctaa cggttacaat gccagcaatc gtaccaccgc acaggatggt
     1201 ttcactttct ccatcatcag cggcaccacc aatggtacca ccgcagtaac cgattacagc
     1261 actctaccgg aaggcatctg gagcggcgac gttagcgtac agttcgacgc tacctggacc
     1321 agttaatctc tctgatgtac cagcaggggt acccccctgc tttattcctg gacggaattg
     1381 ttatgaaaaa gcaccttctg cctctcgctc tgctgttttc cggaatatca ccggcccagg
     1441 cgctggatgt cggcgatata tcatcgttta tgaacagtga cagcagcacg ctaagcaaaa
     1501 cgatccaaaa cagtaccgac agtggccgcc tcatcaatat ccgtctcgaa cggctctctt
     1561 caccgcttga cgacgggcag gttatcgcaa tggacaagcc ggatgagttg ctactcactc
     1621 ccgccagctt gctgctaccc gcccaagcca gcgaagtgat ccgcttcttc tataagggac
     1681 cagcagatga aaaagagcgc taagtgatcc gcttcttcta taagggacca gcagatgaaa
     1741 aagagcgcta ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata
     1801 atgccaaccg tagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg
     1861 caccccgcca ggcaaactac cactttcagt acgccaacgg ctccctgaca aatacaggaa
     1921 atgcgacgct gcggatcctc gcctacggcc cctgcctgaa agccgccaat ggtaaggagt
     1981 gtaaagagaa ttactacctg atgccgggca agtcgcgtcg ttttacccgc gtggacacgg
     2041 cggataacaa aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga
     2101 tagctgtgca gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc
     2161 cagtttgcct tcggcatggt ctttttgttc gttcagcccg atgccagcgc tactgacata
     2221 agtgcgcagc aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gcttcaggac
     2281 ggcatgagcg tcccgctcta tattcatctc gccggtagcc agggtcgcca ggacgatcag
     2341 cgaatcggca gcgcttttat ctggttggat gatggacagt tacgcatccg gaaaatacag
     2401 ctggaagaga gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca actgatgact
     2461 ctggccaacg ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagttg
     2521 gatctcagct tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc
     2581 gtactacgct cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat
     2641 ctgagctata acttcggcgt ctataacaac cagttgcgta acggcgggag caacacctcc
     2701 agctatctgt cgctgaataa cgttactgcg ctgcgcgaac atcacgtggt gctcgacggc
     2761 tcactgtacg ggattggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa
     2821 cgcgattttg ctggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc
     2881 ttagggccga tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag
     2941 gccagctcca ccatcttcga cagcagccaa tcggccacgc cagtgatcgc ctttttaccg
     3001 gcggcgggcg aagtacatct cacccgtgat gggcgactac taagcgttca gaacttcacc
     3061 atgggcaatc atgaagtgga tacccggggt ctaccgtacg gtatttacga tgtggaagtt
     3121 gaggtaatcg ttaacggtcg cgtgatcagc aaacgcaccc agcgggtcaa taagctgttt
     3181 agccggggac gtggcgtcgg tgcaccgctg gcgtggcaga tatggggcgg tagctttcat
     3241 atggatcgct ggtcggaaaa cgggaaaaag acgcgaccag ccaaagagag ttggctggca
     3301 ggtgcctcga cctccggctc actgagtacg tttagctggg cggcaacggg atatggatac
     3361 gataatcagg cggtgggtga aacccgtctg acgctgccgc ttgggggggc gatcaacgtt
     3421 aacctgcaaa acatgctggc cagtgacagc tcatggagca acatcgccag catcagcgcc
     3481 actctacctg gaggcttcag ttcgctgtgg gttaaccagg aaaaaacccg cattggcaat
     3541 caattgcgac gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa cctgaactca
     3601 ctgtggtcga agctgggtac gttcagcatc agctacaatg atgaccgccg ctacaacagc
     3661 cattattaca cggcagatta ctatcaaagt gtctacagcg gtacctttgg ttcgcttggc
     3721 ctgcgggccg gtattcagcg ctataacaac ggcgacagca gcgccaatac agggaaatat
     3781 atcgctctcg atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa
     3841 aacggctaca ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc
     3901 actgttggtg ccaatctgtc acgagccatc tccggcgata ccggtgatga caaaaccctc
     3961 agcggtgggg cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat
     4021 agcgcggcgg acggctacat caatactaat ttgaccgcca acggcagcgt cggctggcag
     4081 ggtaaaaaca tcgatgccag cgggcggacc gatggcaacg ctggggtgat attcgacact
     4141 ggcctggaga acgatggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac
     4201 ggcaagcgta actatctccc gctctctccc tatggaagat atgaggtgga gttgcagaac
     4261 agcaaaaact cactcgacag ttacgatatc gtcagcggtc gcaaaagtca tctgactctc
     4321 tatccaggca atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt
     4381 cgtatccgtg cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc
     4441 cgaacccgaa ccgatgaaaa cggcgagttt gtcatggacg tggataagaa ataccccact
     4501 atcgattttc gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg
     4561 cgcggtgccg tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg
     4621 acgcagacag gagaagagaa tgagagttaa cctactgata gcgatgataa tctttgcgct
     4681 aatctggcct gcaaccgcgc tcagagcggc agtgagcaaa acaacctggg cggatgctcc
     4741 ggcacgcgag tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg
     4801 cggggcgctg gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacaatgg
     4861 ttcaggaacc atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct
     4921 ttataccaac tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac
     4981 tggcttgcgc tgcatcaact ggtacgcagg gtgtaatatg accaccagtc ttatcctgcc
     5041 gcaaaccacc gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg
     5101 gatgcacggc atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag
     5161 cagctttaca atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg
     5221 cgcacgctgt aaggatcagg cctccggcaa ctggtatgtt cgcaacgtca cccatacgaa
     5281 agcagcaaat ctacggttga taaataccca ctcgctggcg gaagtattta tcaacagcga
     5341 cggagtaccg actctgggcg aagggaacgc cgactgccgg acgcaaacca tcggccgtcg
     5401 ttcaggatta agttgtaaga tggttaacta taccctgcaa acaaacggac tcagcaacac
     5461 ctcaatccat atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta
     5521 cgatatgcag ttcagtctga atggcagttc atggaaaccg gtgagcaata ctgcctatta
     5581 ctacaccttc aacgagatga agagcgcaga ctcgatctat gtgttcttct cgagcaactt
     5641 ctttaagcag atggtgaacc tcgggatcag cgatatcaac accaaagatc tattcaactt
     5701 tcgctttcag aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac
     5761 gctgattatc aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac
     5821 accgtcgcgg gagggatatg ttggcagcgg cgagtcggca ctcgatttcg gctatatcgt
     5881 aaccaccagc ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca
     5941 ggtgattggc gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc
     6001 gttcccggcg acgctttcct ttattacccg caacggagct acaaaaacct acgatgccgg
     6061 gtgcgatgat agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga
     6121 tatctctggc gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga
     6181 taacgccatt tctctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc
     6241 aggagagatt catgttcagg cgacgtggcg taacattaac taaggccctg ctgacagtgg
     6301 tctgtatgct ggcggcacct ttgacacagg cgatttcggt cggcaatctg actttttcac
     6361 tgccgtccga gactgacttt gtcagcaaac gtgtcgtgaa taacaacaaa agcgcgcgaa
     6421 tataccgtat tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac
     6481 cggtggatgg tgaactactt ttcgcccccc gccagctggc gttgcaggct ggtgagagcg
     6541 agtattttaa attttactat catggtccac gggataaccg cgagcgctac taccgggtct
     6601 catttcgcga ggtccccact cgtaacctga caaaacgcag cccaaccggc ggtgaggtca
     6661 gcacggagcc ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt
     6721 ttaaatggtc cttcgatcag gtgaccggaa cggtaagtaa caccggcaac acgtggttta
     6781 agctactgat taaaccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac
     6841 gtccgggaga cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct
     6901 ataacgacaa attcattaag attagcgatt cctgtccggc taagccccct tcggcggact
     6961 aagacttatc catcagacga gggaagaatt ccacctcaga gactcaaaaa tcgtttaaat
     7021 gatgtggaag cgatcgtcaa tgtcacctgt aacgatgagc tttctcatat gcacaccaca
     7081 gtgttcagga gacacggaaa ggtaccaggc ggcctgctaa cgagtggttt cttcactaat
     7141 taggacgtaa aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagtacac
     7201 cagccaaccg cgatacgtcc ctgcatacga cgtaccgcag ttctctttac gatttatagc
     7261 tgtactgatg aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat
     7321 ttttccgcgt ctctttttac tgggccgaag ccgacgatgc tggcggtacc attcccgcca
     7381 ggcttactga acaaatatcc ccttttcacc gatgcatgag cggtaccaat cagaaaaacc
     7441 cccgatgtat tgcccttgca ggaacaccgg gcaaaaatgc ctgttgcacg atatataaaa
     7501 atcgatcgtc cacatgcaga gaattcgcca tgtctggtga gaacggagaa gtcaatgagg
     7561 cttgcaatcg tgcaagagct aaatacgggc taacaccgtt ataaatatac aataattaat
     7621 tgcattgccc cgccagctgt aatagcgggg cttcgttttt cagggacaac aaaacctgaa
     7681 acttcttcca ttttccagca acaatgcctc ttcttctgta gcgttctccg gatctacatc
     7741 atcctgaagt agcgcgatat gatcactgac tgtccgcatc agaatatgaa aagcaacgtt
     7801 tttaacctat aaaaatggcg ctatatttgc gccattttta tcattcaaag cattatctat
     7861 ttgagcctaa agggatctca ggatctggct catgggtaat tctgtttcgt aaatctctgc
     7921 gaataatttc aatagaccag aaccagacta aatgtccaaa aatttcagag acattctcat
     7981 accacggaag atcaaagaga ggtggcgtaa gtcccatgag agggaatgaa atcatatgaa
     8041 caaaaagttg cgctaaagca cctgccagta agccctgcca aagtttaatt ttcggaaata
     8101 cttcggcgac cacacagtaa ccaacggcaa aaactatcga aaagattatg tgcgttacac
     8161 caacccagtt aaagacatgc ccggcgaagg tataaacagc cgaatttgga tctgtcagtc
     8221 ccaaccaatc ccgaagaaaa atgtacggag gattgagaaa gttacgggag caatcaattt
     8281 ggcctgcagc cctgattaat gattccggac cacacgctgc attaaacata tcaaccggac
     8341 tacgtggtgg caatggaact tctgcccccc acttaacaaa tgcggaaaca acgccagcaa
     8401 tcagcccaat gaatgcagca agaccataac gtctgcggtt tggtggagtt tgttcaaata
     8461 tattcatatc taccctgctt gtaccattat gttatacacc tctttaggag tattcataaa
     8521 acaaggcaaa tgtaaagaac tgtattgttt tgtataacca catggttacc taatcgccaa
     8581 ttaatataag ctccattatt tctccatatt tttatgttag aagtgacaag gagaaatgta
     8641 atgtttctct cagagaatat aaatcattcc ttttgcccac cttaatggtg ggctttttta
     8701 tgcaaaacct gaacgtcctg tggtattcct ctggcataat gattttcgcg attaaccaac
     8761 aaccagacca gcaaatacgc caccaccagc acagcaacat ccatcaggtt tgccacaccc
     8821 catgcgtgcg gctcaaagcc gccatggcat attaatccgc ttgccatttt cgaacagctg
     8881 ataggtttag tccggtgttt ccctgcttgc acttaaaaat gagcggatgg gaaactctcg
     8941 aaatggttcg tcgatatgct cacctcgcac ctaatcacct taccgaacac gcacggtaaa
     9001 tagattcgat cctgaaccca tcggtcccaa atctgtccca atcaaaaaaa taaggaaggt
     9061 attaatgatg tgtaacttat tgatttaaaa tggataatat aaacagattg taaaaagcaa
     9121 gacattgat
//
LOCUS       DAZGJT010000146         9103 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZGJT010000146 DAZGJT010000000
VERSION     DAZGJT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.149"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHKFK_004300"
     CDS             <1..80
                     /locus_tag="ACHKFK_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9054512.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            97..2601
                     /locus_tag="ACHKFK_004301"
                     /pseudo
     CDS             97..2601
                     /locus_tag="ACHKFK_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
     gene            2660..4720
                     /locus_tag="ACHKFK_004302"
     CDS             2660..4720
                     /locus_tag="ACHKFK_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9054513.1"
                     /translation="MAVQISGVLKDGAGKPIQNCTIQLKAKRNSTTVLVNTVASENPD
                     EAGRYSMDVEYGQYSVTLLVEGFPPSHAGTITVYEGSRPGTLNDFLGAMTEDDVMPEA
                     LRRFEVMVEDVARNAEAASQSAAAAKKSETAAASSRNAAKTSETNAGNSAKAAASSKT
                     AAQNAATAAERSETNARASEEASADSEEASRRNAESAAENAGVATTKAREAAADATKA
                     GQKKDEALSAATRAEKAADRAEAAAEVTAEPYANIVPPLPDVWIPFNDSLDMIAGFSP
                     GYKKIAIGDDVVQVASDKQVNFSRASTATYINKSGELKTAEINEPRFECDGLLIEGQR
                     TNYMLNSESPASWGKSSNMDVPETGTDSFGFTYGKFVCNDSLVGQTSAINMASIAATK
                     SVDVSGDNKYVTTSCRFKTERQVRLRIRFDKYDGSATTFLGDAYIDTQTLEINMTGGA
                     AGRITARVRKDKTTGWIFAEATIQAIDGELKIGSQIQYSPEQGGATVSGDYIYLATPQ
                     VENGPCVSSFIISGGSATTRASDLVSIPTRNNLYKLPFTFLLEIHKNWDIAPNAAPRV
                     WDIAAANTGQSAIAAINRGSGKLYMSLSNPSGSYVNSAATDVFAEKTTFGCIAKADGH
                     FHVVTNGKAVNEVYCEYNGVTADKNIRFGGQTNTGERHLFGHIRNFRIWHKELNDRQL
                     KEVV"
     gene            4717..4995
                     /locus_tag="ACHKFK_004303"
     CDS             4717..4995
                     /locus_tag="ACHKFK_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054514.1"
                     /translation="MKDLTLKFHDKLQFKAFLSSLGWAEDEDLQNKLLVDEIGFTYTE
                     TGVTEEGEPVCIRNDGYFVNIRILDDLFDVSVFSDYVVELETPLREWS"
     gene            5008..5298
                     /locus_tag="ACHKFK_004304"
                     /pseudo
     CDS             5008..5298
                     /locus_tag="ACHKFK_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5393..6250)
                     /gene="sitD"
                     /locus_tag="ACHKFK_004305"
     CDS             complement(5393..6250)
                     /gene="sitD"
                     /locus_tag="ACHKFK_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO9054515.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTMIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVLFIMTFAVTSINARKKGNS
                     HTQDLLSPN"
     gene            complement(6247..7104)
                     /gene="sitC"
                     /locus_tag="ACHKFK_004306"
     CDS             complement(6247..7104)
                     /gene="sitC"
                     /locus_tag="ACHKFK_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO9054516.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKLLFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(7101..7928)
                     /gene="sitB"
                     /locus_tag="ACHKFK_004307"
     CDS             complement(7101..7928)
                     /gene="sitB"
                     /locus_tag="ACHKFK_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO9054517.1"
                     /translation="MMQSAGIVVNDVAVTWHNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVNFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(7928..8842)
                     /gene="sitA"
                     /locus_tag="ACHKFK_004308"
     CDS             complement(7928..8842)
                     /gene="sitA"
                     /locus_tag="ACHKFK_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO9054518.1"
                     /translation="MRSIKKVTMLLGSLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         2423 a         2161 c         2445 g         2074 t
ORIGIN      
        1 agtcgctgtc gttctcaaaa tcggtggagc tgcatgacaa agtcatcggg cattatctga
       61 acataaaaca ctatcaataa gttggagtca ttaccatggg cgctgtatgt catcggccag
      121 aattgcgacc agtcggtgcc ggatggtttt ggtggcacgg agccgcgcat cacctgtaat
      181 gcctggctga ccacacagcg taaggcgtgg gatgttctca gtgatttctg ctcggcgatg
      241 cgctgtatgc cggtatggaa cgggcagacg ctgacgttcg tgcaggaccg gccgtcggat
      301 aaggtgtgga cctataaccg cagtaatgtg gtgatgccgg atgatggcgc gccgttccgc
      361 tacagtttca gcgccctgaa ggaccgccat aatgccgttg aggtgaactg gattgacccg
      421 gataacggct gggagacggc gacagagctt gtggaggaca cgcaggccat tgtccgttac
      481 ggtcgtaacg tcacgaagat ggatgccttt ggctgtacca gtcgggggca ggcacaccga
      541 gccgggctgt ggctgattaa aacggaactg ctggaaacgc agaccgtgga tttcagcgtg
      601 ggcgcagaag ggcttcgcca tgtgccgggc gatgttattg aaatctgcga tgatgactat
      661 gcgggtatca gcatcggcgg gcgcgtgctg gcggtgaaca gccagacccg gacgctgacg
      721 ctcgaccgtg aaatcactct gccatcctcc ggtaccacgc tgataagcct ggttgacgga
      781 agcggcaatc cggtcagcgt ggaggtccag tccgtcaccg acggcgtgaa ggtaaaagtg
      841 agccgggttc ctgacggcgt tgccggatac agcgtgtggg ggctgaagtt gccgacgttg
      901 cgccagcgcc tgttccgctg cgtgagtatc cgtgagaacg atgacggcac gtatgccatc
      961 actgccgtgc agcatgtacc ggaaaaagag gccatcgtgg ataacggggc gcactttgac
     1021 ggcgaccaga gcggcacggt gaatggtgtc acgccgccag cggtgcagca cctgactgcc
     1081 gaagtcaccg cagacagcgg ggaatatcag gtgctggcgc gctgggacac gccgaaggtg
     1141 gtgaaggggg tgagctttat gcttcgcctg accgtggcag cggatgacgg cagtgagcgg
     1201 ctggtcagca cggcccggac gacggaaacc acataccgct tcacgcaact ggcgctgggg
     1261 cggtacacgc tgacagtccg ggcggtaaat gcgtgggggc agcagggcga tccggcgtcg
     1321 gtatcgttcc ggattgccgc accggcagca ccgtcgagga ttgatctgac gccgggctat
     1381 tttcagataa ccgccacgcc gcatcttgcc gtttatgacc cgacggtaca gtttgaattc
     1441 tggttctcgg aaaagcggat taccgatatc aggcaggttg aaaccacagc ccgctatctt
     1501 ggcacggcgc tgtactggat agctgccagt atcaatatta agccgggcca tgattattat
     1561 ttttatatcc gcagtgtgaa tactgttggc aaatcggcat tcgtggaggc tgtcggtcag
     1621 gcgagcgatg atgcttccgg ctatctggat tttttcaaag gccagataac tgaatcccat
     1681 ctcggcaagg agctgctgga aaaagtcgag ctgacggagg ataacgccag caaactggag
     1741 gagttttcga aagagtggaa agacgccaac gataaatgga atgccatgtg gggcgtcaaa
     1801 attgagcaga ccgaagacgg caggcattat gtcgcgggta ttggcctcag catggaggat
     1861 acggaggaag gcaaactgag ccagttcctg gttgccgcta accgtatcgc gtttattgac
     1921 ccggcaaacg ggaatgaaac gccgatgttt gtggcgcagg gcaaccagat attcatgaac
     1981 gaagtgttcc tgaagtatct gacggctccc accattacca gcggcggcaa tccgccggta
     2041 ttttccctga caccggacgg gcggttgacg gcgaaaaatg ccgatatcag cggtaacgtg
     2101 aatgcgaact cagggacgct caataatgtc acgattaacc agaactgtcg gattctggga
     2161 aaactgtctg ccaaccagat tgaaggtgat attgtcaaaa cggtgggaaa agcctttccg
     2221 agaaatggca gttatgccag cggtacaata acggtcactg tgtacgatga ccaggctttt
     2281 gaccgtcaga tagtaatccc acccgttctg tttcgcggtg gtaagcatga aaacttcaac
     2341 agcaacaacc aacagtcata ctggtattca acctgtaagc tgcaggtact gaagaacgga
     2401 caggaaatct ttcagcaacc cgcgacggat gtcagcaggg tattttcatc cgtcattgat
     2461 atgccagcgg gacacggcca tgtcaccctg actttcaatg tttcttcata tggtgcaaat
     2521 aactggacgc caacgaccag tatcagcgac cttcttgttg tcgtgatgaa gaaatcaaca
     2581 gccggtatca gtatcagttg aattttataa cccaaatacg ggcgccagaa atggcgcctt
     2641 ttttatttgt ggagtgaata tggcagtaca gatttcaggc gtgctgaaag acggtgcagg
     2701 aaaaccaata cagaactgta ctattcagct caaagcaaag cgtaacagca ccacggtact
     2761 ggtgaacacg gtggcctctg aaaatccgga tgaagccggg cgttacagca tggatgttga
     2821 gtatggccag tacagcgtca ccctgctggt tgaaggtttt ccgccttcac atgccgggac
     2881 cattaccgtc tatgaaggtt ccagaccagg tacgctgaat gattttctcg gtgccatgac
     2941 ggaagatgat gtcatgccgg aggcattgcg tcgttttgag gtaatggtgg aggatgtggc
     3001 acgcaacgct gaagccgcct ctcagagcgc agcggcagca aagaaatcag aaacagcagc
     3061 ggcatcgtcc aggaacgcgg cgaaaacatc agagacgaat gcaggtaaca gcgcgaaagc
     3121 ggcagcttct tcaaaaacag ccgcacaaaa cgcagcaaca gcggcagaac gttcagagac
     3181 aaatgcccgt gcgtcagaag aagcctccgc agacagtgaa gaggcttccc gccgtaatgc
     3241 agagtcagcc gctgaaaatg ccggagtcgc caccacaaaa gcgcgggagg ctgcagcaga
     3301 cgcaacaaag gccgggcaga aaaaggatga ggctctgtcg gcagcgacac gagctgaaaa
     3361 ggcggcagac cgcgcagaag ccgcagcgga agtgactgca gagccctatg cgaatatagt
     3421 gccgccgctg cctgatgtgt ggataccgtt taacgattca ctggatatga ttgcgggttt
     3481 ttctccgggc tataaaaaaa tagctattgg tgacgatgtg gttcaggtcg ccagtgataa
     3541 acaggttaat ttcagtcgcg catcaacggc aacatatatc aacaaatctg gcgaactgaa
     3601 aacggcggaa attaatgagc cgcgatttga gtgtgatggc ctgcttattg agggacaaag
     3661 aacgaactac atgctcaatt cggaaagtcc agccagctgg gggaagtcat caaacatgga
     3721 tgtgcccgaa accgggacgg atagttttgg ttttacttat ggaaagtttg tctgcaacga
     3781 ttctctggtt gggcaaactt cggctattaa tatggcatca attgctgcaa caaagtcagt
     3841 tgatgtctca ggcgataaca agtacgtgac aacctcatgc cgttttaaaa cagaacgaca
     3901 ggtaaggtta cgtatacggt ttgataagta tgatggtagt gcaacaactt ttcttggcga
     3961 tgcgtacatt gatacgcaaa cgcttgaaat taatatgaca ggtggtgctg ccggcagaat
     4021 tacggcacga gtcaggaagg ataagaccac gggctggatt tttgcagagg caacgattca
     4081 ggcaattgat ggagagttaa aaataggctc tcagatacag tattctcctg agcagggtgg
     4141 ggcaacagta tctggtgact atatttatct tgccacccca caagtagaga atgggccatg
     4201 tgtatcatca tttattattt caggaggcag cgcaacgaca agagccagtg atttggttag
     4261 tatccccacc agaaataatc tttataagtt accatttact tttttacttg agattcataa
     4321 aaactgggat attgcaccaa acgccgcacc ccgcgtgtgg gatatagcag cagccaatac
     4381 cgggcaatca gcaattgcag caatcaacag aggtagtggt aagttatata tgagtctgtc
     4441 aaacccttca ggctcgtatg ttaatagcgc agcgacagat gtatttgcag agaaaaccac
     4501 atttggatgt attgcaaaag ctgatggtca ctttcatgtg gtgacaaatg gtaaagcggt
     4561 taatgaagtt tattgtgaat ataatggcgt gaccgctgat aaaaatatcc gatttggagg
     4621 gcagacgaat actggagaac gacatctgtt tggccatatt cgcaatttcc gcatatggca
     4681 taaagaatta aatgacaggc aattaaaaga ggtcgtatga aagatttaac tttgaagttt
     4741 catgacaaac tgcagtttaa ggccttcctg tcatctcttg gctgggcgga agatgaagac
     4801 ctccagaata aactgttagt tgatgaaatt ggtttcacct acacagaaac aggggtaaca
     4861 gaagagggag aacctgtctg tatccggaat gatggttatt ttgtcaacat tcgcattctt
     4921 gatgacttgt ttgatgtttc tgtattctct gattatgtcg tggagctgga aacaccgctt
     4981 cgggaatgga gctgaaagga ggaaataatg gatataagcc ccttacttca tgcactttgt
     5041 gctgtggctg cgcagatact ggttggtctt tttaccggaa actgggctta cggagcgata
     5101 gccggttgta cgttcttcat tgcgcgtgaa catacccagg cagaatatcg ctggattgaa
     5161 atgttcgggc atggcaagcg aatgaatatg ccgtggtggg gcggttttga tccgcgtgct
     5221 tgggatgtgg caagcctgat ggattttgct gtgccggtgg tggcgtgtct gctggtctgg
     5281 ctgttggtta atcgtgggcg aaaaaaggtg agctgtatat gcaacggagg aagaaacccc
     5341 gttgctggaa gccaggaaaa agtatcgggt gttgctgtcc tcggatgttg tgctaattgg
     5401 gtgataacag atcctgtgta tgggagtttc cctttttgcg tgcgttgatg ctggtaacgg
     5461 caaacgtcat gatgaacaaa accgcgaata agacgacaat cgtcggagct ggtgcgctgt
     5521 cgataaaaaa ggacaaccag acaccagtca ttgacactat taccgatatg ccagtcgcca
     5581 gtaggagcgc aatatgaaag cgttgtgtca ggagtaccgc aatggcacca ggcgcaatga
     5641 gcagagaaat ggagagaatg atacctaccg ctttcagcgt cgccacaatg gtcagagaga
     5701 ccatgcacag cagcccataa tgcagccatc tcgtatgcaa accgcttacc tgcgcctgtt
     5761 gataatcaaa gctgaacagc aaaaaatccc gccattttac gctaataaca agtgtaacca
     5821 gcccggcaat aatcatcgtc tggattatat cgccgatggt tatcccgagc atgtcgccga
     5881 aaagaatatg gtcaagatgt acgtctggtt tgactgcaat atacaagatc agacctgcgg
     5941 caaacatgcc agaaaaaaca atccccatca ctgtatcttg cttgattcgg ctgttgtctt
     6001 tcaggtatcc cgtcgccacc gcgcagaata ctccggcgac gaatgcccct gtagccaaag
     6061 gtaaccccag aatccaggca agcacgatgc caggaaaaac cgcatgactc atcgcgtcac
     6121 ccatcagtgc ccaacctttc aggaccagaa agactgacag cagcgcacag ggtatggaaa
     6181 ctaccagtga aatcagcagt gcgtgactca taaaggtgaa ctgcagtggc tccagtaata
     6241 atgccatcat gagcgatcct ccagcgcctt atgcgcacgc cgacgatttg ccaaaagccc
     6301 atgggtgggc gcaaagacaa atgctaaaag aaacagcagt gtctgcgcca ccacgatgat
     6361 tccacctgtc gcgccgtcca gaaagtaact gacccacgcg ccgaggaaac tggttacact
     6421 gccaatagtt acagcaatca tgagcaggcg gggaaaacga tcggttaaca accatgctgt
     6481 tgcaccggga gtcaccacca gacagatcac cagaaaggca ccaaccgttt gcagcgccgc
     6541 aacggtcgaa accgacagca gggtgaagaa gaggagtttt aatctgcccg gatgcaatcc
     6601 aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata agatctttcc atttgaaaaa
     6661 aagcacgatg atcgagagaa tgccaatgat agtgagttgc aggatatccg ccggatcgat
     6721 tgccagaata ttgcccagga cgatggtctg aatgttcaca gatgtcgggt tcaatgacac
     6781 cataaacagc cccaggccaa aaaaggacga gaagatcaga ccgatgatgg catcttcttt
     6841 taagcgggtg cgttggttaa gaaacagcat gctgcccgca gccagtccgc cggaaaagaa
     6901 ggctcccaga gaaaacggaa gccccagcat atatgccccc gcaacacccg ggacaataga
     6961 gtgcgacaac gcatcgccaa tcagcgacca gccttttagc atcagatagc atgacaggaa
     7021 agcgcagaga ccgccgacca tcgccgagac ccacatcgca ttgagcatat attcatagct
     7081 gaagggttcc agtagcacgt tcatctttct tccctctgca ccgctgacgg tcgatgagcc
     7141 acaaaagggc gttcatcgtc agtaataatg ctttcttccg agccgttaag tgtgacatgg
     7201 cgcagtacgc cgctaaaagc cagctccagg ttctcggctg taaaagtggt gtctgtcggc
     7261 ccgcttgcca ggacggtgcc tttgaccatg accgtataat cgcaaaatgt cgtgacggat
     7321 ccgagattgt gtgttgagac aagcatcgtt ttgccttccg cgcgtaactc cctcaacagg
     7381 ctgatgattt tggcttcggt tttcacatca acgccagtaa aaggttcatc cagcaggatc
     7441 acatcaccct gctgtgcaat cgctctcgcc agaaagacgc gtttcttttg tccaccggaa
     7501 agctcgccga tttgtcgatg gcgaaaattc accatatcga cacgttccag cgcatcggtg
     7561 acaatttgac gatccctctt tttggcgata cgcaacatac ccatatgccc atagcggccc
     7621 atcatcacca catcttccac cagtacagga aatgaccagt caacctcttc tgactgaggg
     7681 acgtaggcaa ccaggttttt ctgtaacgcc tgtcgcgtgg gaatacccag aacagatatc
     7741 tttccgctgg tcagacgcac aaatcccata atcgctttaa acaacgtcga ttttccggaa
     7801 ccgtttaccc caaccagagc ggcaatagat ccgcctggta ctgtgaagga tgcatcgcgc
     7861 agtgctgtgt gcccgttatg ccaggtgaca gcaacatcat taacgacaat gcctgcagat
     7921 tgcatcatta tttctccctt ttcccggctt taattccctg taccagcgta ctggtagtaa
     7981 ccttaagaag gtcgatatac gtcggtaccg ggccgttttc tgtgctcagg gaatcgacat
     8041 agagcacacc gccgtagtgt gcgccggttt cacgcgcaac ctgacgcgct ggtttatcgg
     8101 aaatcgtact ctcgctaaag actgccggga tatgattttt cttaactata tcaacaacct
     8161 tacgtacctg ctgcggtgtt ccttgttgat cggcattaat cggccacaga taaagctctt
     8221 ttagccccaa atcgcgtgcg agataagaaa aagccccttc actggtgacc atccatcgct
     8281 gattctcagg gagttccgta atctgcttac gcaggggggc aagggtttgg gtaatcttgg
     8341 ctttataagt atcggcattg cgttggtagg tttgtgcatt tgccggatcg tattttatca
     8401 acgcatcacg aatattatcg acgtaaatca gagcattatc tggcgacatc catgcatggg
     8461 ggttaggttt gccctcatag ggtccttcgg tgatccctac aggcgtcaca cccgaagaga
     8521 caattacttc tggaacccca ttgagatgct ggtaaaagcg ttggaaccac aattccagat
     8581 tcataccatt ggcgagaatc agttgtgccc cctgcgcacg tttaatatcg ccaggggtag
     8641 gctgatactc atgaatttct gcaccaggct tggttatgga tgagacttct gcagcatctc
     8701 ccgccacgtt tttggccata tccgcgatga tggtaaatgt tgtaatgacc ttaaattttt
     8761 cagttgcact tgcctgaaat gcgatcgagc aggtgagtgc gaggctcccc aagagcatgg
     8821 ttactttttt tattgagcgc ataatttagt acctatagtg agcaaaatta gaacaaacat
     8881 agcaacggct atgttttata acataaccta tacataatga aaatgattat cattagcatt
     8941 tttgaggccc aaaacaggga ttagtttgtt tcctgctgga tgaagggact cttgggatag
     9001 cacatgccgc acgaagagca gcattacatg atgttttgtc gaatgagcta cgaatcctga
     9061 tagaaatacg aaacaagcta gcacatggac aatggatcta tcc
//
LOCUS       DAZGJT010000147         9075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZGJT010000147 DAZGJT010000000
VERSION     DAZGJT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.150"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(112..936)
                     /locus_tag="ACHKFK_004309"
     CDS             complement(112..936)
                     /locus_tag="ACHKFK_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO9054519.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            1256..3286
                     /gene="malS"
                     /locus_tag="ACHKFK_004310"
                     /pseudo
     CDS             1256..3286
                     /gene="malS"
                     /locus_tag="ACHKFK_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            3464..4717
                     /gene="avtA"
                     /locus_tag="ACHKFK_004311"
     CDS             3464..4717
                     /gene="avtA"
                     /locus_tag="ACHKFK_004311"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO9054520.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(4869..5342)
                     /locus_tag="ACHKFK_004312"
     CDS             complement(4869..5342)
                     /locus_tag="ACHKFK_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO9054521.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHTYTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQSRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRATGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(5444..6292)
                     /gene="yiaJ"
                     /locus_tag="ACHKFK_004313"
     CDS             complement(5444..6292)
                     /gene="yiaJ"
                     /locus_tag="ACHKFK_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO9054522.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWESHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            6493..7491
                     /gene="yiaK"
                     /locus_tag="ACHKFK_004314"
     CDS             6493..7491
                     /gene="yiaK"
                     /locus_tag="ACHKFK_004314"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO9054523.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTEDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTSAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            7503..7967
                     /locus_tag="ACHKFK_004315"
     CDS             7503..7967
                     /locus_tag="ACHKFK_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO9054524.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRTTDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPACNKNATTAIRKIVVKVALAAL"
     gene            7990..8910
                     /locus_tag="ACHKFK_004316"
     CDS             7990..8910
                     /locus_tag="ACHKFK_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4862 family protein"
                     /protein_id="HFO9054525.1"
                     /translation="MKTNNAGYIIGAYPCAPSFHQRSEEEEKDFWRQLSETPDILGLE
                     QPCLENFHPLGDQWLLRHTPESWKFVVTAVMETMRRRSENSGFGLASSDEEQRQACVA
                     YYRHLFNKINSLQANKVLALELQAAPLATNPNVMQATDAFARSLKEIASWDWPCKLVL
                     EHCDAMTSSSPRKGFLPLENVLEVITDYDISICINWARSAIEGRNTTLPLTHTQMAKQ
                     AGKLGALMFSGTTLNGAYGEWQDLHAPFAPFCAESLMTTDHVRELFNVAESSTLHFAG
                     IKLLEINATADVHHRIEILRNGIHSLNESR"
BASE COUNT         2315 a         2307 c         2233 g         2220 t
ORIGIN      
        1 gtagcgatga tgagaattat cggcgtcgca tcaggtaact cagcacaacc acctgatacg
       61 agcaaaagcc ttatccgcct gacaagtcgg ataaggctcg aggaaatgca attacatatg
      121 cgccgcgatt aaccgttggt tatcctggta cattgcgaac aggtagttgt tataactctt
      181 ccctttggtc gagtagccct tcagcttgtg aatcatcgct gtggcagtca cttcctgatc
      241 cgctttacgc agttgtgcac gcgatttacg gaacgaagag taagccgggt gcgtattgag
      301 gttagtgaca taggcgctca ccgattcttt aacagaacta aactgtgagt accctttcac
      361 tttacccggc gcattggtac aacgtccttt catgcatttc atgccgaaca ggttgttgtt
      421 gttgcgcgcc agctttgacg ttccccaacc gctttctgct gcagccatcg tcgccaccat
      481 actggtaggg ataatgtcta cgcgttcaag caaggtattc cacgggattt ttcgcgtatt
      541 accagaccac ttcaccttgt agcgtttggc gatgtctttc agacgcgcac gctcagcagg
      601 tgaccattgg ccctgatact gttttgaaat cagccagtta cgttccgcag taatggccgc
      661 attttggctg gtaatgtaag gcattacagt ccggagaaac gcctttttcc ttggtgttcc
      721 ggaagggtat tttcgcaaat caggaagtga actactcttt gcactattgc gagaatactc
      781 ttgtttactg cttacctgtt tattactagc tttagttaag tgggactttt gactcgctgt
      841 tgttgtgtgc gtcttcgcta acacctcact cgaaaacacc agagtgagta acataagaat
      901 catcgcccca tatcgtcgta tgggagtcaa aatcatcagg tctcctggtc ggatttaatc
      961 attccaacac cttatatttt ttacaaattt gagagttgaa tctcaaatca tatcaaaaat
     1021 agctgtcaag agcaccccaa ggaatagtcc aaatctgaaa ctatgtcacg agttaacgat
     1081 tcagattggc gctaaatcgc agaaaatgtg gggtttatcg caaaattcag ccgttttttg
     1141 cgcgcgatcg ctcacccttg cttctcatcc tgtggactta ccgctcaggg atgagttttg
     1201 tctggcttat cgctggcaaa ctgtctgaaa tcgcagcaat aaggactcat ccgccatgaa
     1261 actcgccgcc tgttttctga cactccttcc tggcttcgcc gttgccgcca gctggacttc
     1321 tccggggttc cctgccttta gcgaacaggg gacgggaaca tttgtcagcc acgcgcagtt
     1381 gcccaaaggt acgcgtccac taatgctaaa ttttgaccaa cagtgctggc agcctgcgga
     1441 tgcgataaaa ctcaatcaga tgctttccct gcaaccttgt agcaacacgc cgcctcaatg
     1501 gcgattgttc agggacggcg aatatacgct gcaactagac acccgctccg gtacgccaac
     1561 attgatgatt tccatccaga acgccgtcga accggtagca agcctggtcc gtgaatgccc
     1621 gaaatgggat ggattaccgc tcacactgga tgtcagcgcc acttttgcgg agggagccgc
     1681 tgtccgggat tattacagcc agcaaattgc gatagtgaag aacggtcaaa taacgttaca
     1741 acccgctgcc accagcaacg gtttactcct gctggaacgg gcagaaactg acacatccgc
     1801 ccctttcgac tggcataacg ccacggttta ctttgtgctg acagatcgtt tcgaaaacgg
     1861 cgatcccaat aatgaccaga gttacggacg tcataaagac ggtatggcgg aaattggcac
     1921 ttttcacggc ggcgatttac gcggcctgac caacaaactg gattatctcc agcagttggg
     1981 cgttaatgct ttatggataa gcgccccatt tgagcaaatt cacggctggg tcggcggcgg
     2041 tacaaaaggt gatttcccgc attatgccta tcatggttat tacacacagg actggacgaa
     2101 tcttgatgcc aatatgggca gtaaagccga tctacggacg ctggttgata gcgcgcatca
     2161 gcgcggtatt cgtattctct ttgatgtcgt gatgaaccac accggctatg ccacgctggc
     2221 ggatatgcag gagtatcagt ttggcgcgtt atatctttct ggtgacgagc tgaaaaaaac
     2281 gctgggtgaa cgctggagcg actggaaacc tgccgccggg caaacctagc atagctttaa
     2341 cgattacatt aatttcagcg acaaaacagg ctgggataaa tggtggggaa aaaactggat
     2401 cagaacggat atcggcgatt acgacaatcc tggattcgac gatctcacta tgtcgctggc
     2461 ctttttgccg gatatcaaaa ccgaatcaac tagcgcttct ggtctgccag tgttctataa
     2521 aaacaaaacg gatacccacg ctaaagacat cgacggcttt acccctcgcg attacttaac
     2581 ccactggtta agtcagtggg tccgcgacta tgggattgat ggttttcggg tcgataccgc
     2641 caaacatgtt gagttgcccg cctggcagca actgaaaacc gaagccagcg ctgcacttcg
     2701 cgaatggaaa aaagccaacc ccgacaaagc attagatgac aaacctttct ggatgaccgg
     2761 tgaagcctgg ggccacggcg tgatgcaaag tgactactat tgctacggct tcgatgcgat
     2821 gatcaatttc gattatcagg agcaggcggc gaaagcagtc gattgtctgg cgcagatgga
     2881 tacgacctgg cagcaaatgg cggagaaatt gcaggatttc aacgtgttga gctacctctc
     2941 gtcgcatgat acccgcctgt tccgtgaagg gggcgacaaa gcagcagagt tattattatt
     3001 agcgccaggc gcggtacaaa tcttttatgg cgatgaatcc tcgcgtccgt tcggtcctac
     3061 cggttctgat ccgctgcaag gtacgcgttc ggatatgaac tggcaggatg ttagcggtaa
     3121 atctgccgcc agcgtcgcgc actggcaaaa aatcagccag ttccgcgccc gccatcccgc
     3181 aattggcgcg ggcaaacaaa cgacactttc gctgaagcag ggctacggct ttgttcgtga
     3241 gcatggcgac gataaagtgc tggtcatctg ggctgggcaa cagtgacttt tccggcttcc
     3301 cgttcgtcag tacctcggga agccgccaac caggataaaa tgtcagccct aatcagcatt
     3361 gcaggataaa gcaccgctca ctcttcaaca gagcgatttg caccccagca aatgtagcgt
     3421 tattgttacc ttccttgcta cagagttcga cagatatccc gctatgacat tctccctttt
     3481 tggtgacaaa tttacccgcc actccggcat tacgctgctg atggaagatc tgaacgacgg
     3541 tttacgcacg cctggcgcga ttatgctcgg cggcggtaat ccggcgcaga tcccggaaat
     3601 gcaggactac ttccagacgc tactgaccga catgctggaa agtggcaaag cgactgatgc
     3661 actgtgcaat tatgacggtc cacaggggaa aacggagcta ctcacactgc ttgccggaat
     3721 gctgcgcgag aagttgggct gggatatcga accacagaat attgcactaa caaacggcag
     3781 ccagagcgcg tttttctact tatttaatct gtttgccgga cgccgtgccg atggtcgggt
     3841 caaaaaagtg ctgttcccgc tggcaccgga atacattggc tacgctgacg ccggactgga
     3901 agaagatctg tttgtctctg cgcgtccgaa tattgaactg ctgccggaag gccagtttaa
     3961 ataccacgtc gattttgagc atctgcatat tggcgaagaa accgggatga tttgcgtctc
     4021 ccggccgacg aatccaacag gcaatgtgat tactgacgaa gagttgctga agcttgacgc
     4081 gctggcgaat caacacggca ttccgctggt gattgataac gcttatggcg tcccgttccc
     4141 gggtatcatc ttcagcgaag cgcgcccgct atggaatccg aatatcgtgc tgtgcatgag
     4201 tctttccaag ctggggctac ctggctcccg ctgcggcatt atcatcgcca atgaaaaaat
     4261 catcaccgcc atcaccaata tgaacggcat tatcagcctg gcacctggcg gtattggtcc
     4321 ggcgatgatg tgtgaaatga ttaagcgtaa cgatctgctg cgcctgtctg aaacagtcat
     4381 caaaccgttt tactaccagc gtgttcagga aactatcgcc atcattcgcc gctatttacc
     4441 ggaagatcgc tgcctgattc ataaaccgga aggagccatt ttcctctggc tatggtttaa
     4501 ggatttgccc attacgaccg agcagctcta ccagcgcctg aaagcacgcg gcgtgctgat
     4561 ggtgccgggg cacaacttct tcccgggact ggataaaccg tggccgcata cgcatcaatg
     4621 tatgcgcatg aactacgtac cagagccgga gaaaattgag gcgggtgtga agattctggc
     4681 ggaagagata gaaagagcct gggctgaaag ccactaaaaa ttgcctgatg cgctacgctt
     4741 atcaggccta catgatttct gcaatatatt gaatttggta gaatttgtag gccggataag
     4801 gcgttttagc cgcatccggc ataaacaaag cgcactatcc ctgcgttgag aaacgccgga
     4861 aaaacgtttc aaacaggctg ctgccgtagc cgatgccgct gcactttctc gacatccatg
     4921 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtcgc ccgatgccag
     4981 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
     5041 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgattgttc aacgaaaata
     5101 tgcccatgtt cgcggcttat cgcgtcaaca gggcacacat ttgcgcacgg tgcatcttca
     5161 cactgatgac aggctactgc cgtggtgtaa gtatgatctt taatgacacg aatacgggaa
     5221 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
     5281 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
     5341 atgatcactc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
     5401 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gggttatgta atcgcgccct
     5461 ggtcgtccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgaagtg
     5521 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
     5581 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
     5641 gttcgttttc ttctctgtcc atcgccgctc cactttcacg aatgtgcgcc agttcgtcga
     5701 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggc
     5761 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
     5821 cggaacagta gagcggcata tgctggccta tataggcacg ggttcgcagc atcccggttg
     5881 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatag
     5941 tttcaccagt ggcgatgttc agagcctcaa gatgcggagc ggcgatatga atgatattca
     6001 gcgaagatag cgccttctgc ccgacggcaa taaatttggt ggtcaggcga taactccctg
     6061 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
     6121 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
     6181 tgctcaaaat ctcaatcagc atcaacccac gaaacagact ctggcttccg gctgggcgct
     6241 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
     6301 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
     6361 cttgattttt aagttttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
     6421 atatttgaaa tcaagtttcg tatattgaaa ttttaagcca aaaaagcgat caaaaaaaca
     6481 aggaagcctg ggatgaaagt gacatttgag cagttaaaag cagcctttaa tcgggtctta
     6541 atttcacgcg gcgttgacag cgaaacggct gacgcctgtg cagagatgtt cgcccgcacc
     6601 accgaatcag gcgtttattc tcacggcgtt aatcgtttcc ctcgtttcat tcaacaactg
     6661 gaaaacggcg atatcattcc tgatgcccaa cccaaacgta taaccagcct cggcgcaatt
     6721 gaacagtggg acgcccagcg ttcgatcggt aacctgacag cgaaaaagat gatggatcgc
     6781 gccattgaac tggctgccga tcacggtatt ggtctggtgg cactacgtaa tgccaaccac
     6841 tggatgcgcg gcggcagcta cggctggcag gcggcggaaa aaggctatat tggcatttgc
     6901 tggaccaact ccatcgccgt aatgccaccg tggggcgcaa aagagtgtcg catcggcacc
     6961 aacccgctga tcgtcgccat tccttccacg ccgatcacca tggtcgatat gtcgatgtcg
     7021 atgttctctt acggcatgtt agaagttaat cgcctggctg gtcgtcagct cccggtagat
     7081 ggtggctttg atgatgaggg caatttgacc aaagaacctg gcgttatcga gaaaaatcgc
     7141 cgcattttgc cgatgggcta ctggaaaggt tcgggcatgt cgattgtgct ggatatgatc
     7201 gctaccctcc tttctgacgg cgcatcggtt gccgaagtca ccgaggacaa cagcgacgaa
     7261 tacggcattt cacaaatttt tattgccatt gaagtggaca agcttatcga cggtcccacc
     7321 cgcgatgcca aactgcaacg cattatggat tacgttacca gtgccgagcg tgctgacgaa
     7381 aaccaggcta ttcgtttacc cggccacgaa tttactaccc tgctggcaga aaaccgccgt
     7441 aacggtatta ccgttgatga cagcgtgtgg gcaaaaatcc aggcgttata aggagataag
     7501 ccatgatttt tggacatatc gcgcagccta atccgtgccg tttgcccgcc gccattgaaa
     7561 aggcgctcga ttttctgcgc accaccgact tcaacgccct ggaaccgggc gttgtcgaaa
     7621 tcgacggcaa gaatatttat gcgcaaatta tcgatttaac cacacgcgag gcggtagaaa
     7681 accgtccgga agtgcatcgt cggtatatcg atattcagtt tctggcgtgg ggtgaagaga
     7741 aaatcggcat tgctattgat acgggaaata ataaagtcag cgaatcacta ttagagcagc
     7801 gcgatattat tttttatcac gatagtgaac atgaatcatt tatagaaatg attccgggca
     7861 gctacgctat tttcttcccg caggatgttc atcgcccggc atgtaataaa aatgccacta
     7921 cggcaatacg caagatcgtg gttaaagtgg ccctcgctgc tttataaaaa atgattactg
     7981 gagcacataa tgaaaacgaa taatgccggt tatattatcg gcgcgtatcc ctgtgcacct
     8041 tcttttcacc aaagaagtga agaggaagag aaggatttct ggcgacaatt atctgagact
     8101 ccagacattt tggggctgga acaaccttgc ctggaaaatt ttcaccctct tggcgatcaa
     8161 tggttactcc gtcatacgcc agagagctgg aaatttgtcg tcaccgcagt gatggaaacc
     8221 atgcgtcgcc gcagcgaaaa cagcgggttt ggtctcgcct ccagtgatga agaacagcgc
     8281 caggcctgcg tggcgtacta tcgccacctg ttcaataaaa tcaactcatt gcaggctaat
     8341 aaagtgctcg cactggagct ccaggctgca ccgttagcaa cgaatcctaa tgttatgcaa
     8401 gcgacggacg cttttgcccg ctcattgaaa gaaatcgcca gttgggactg gccgtgcaag
     8461 ctggtgctgg aacactgcga cgccatgaca tcttcttccc cccgaaaagg ctttttgcct
     8521 ttagaaaatg tgctggaagt tattaccgat tatgacatca gcatctgtat caactgggca
     8581 cgctctgcca ttgaagggcg caatacaacg ctgccgctga cccacaccca aatggcgaaa
     8641 caagccggaa aactcggcgc gttaatgttc tctggtacga cgctaaacgg ggcatacggc
     8701 gaatggcagg atttacacgc accgtttgca ccattctgcg cagaaagcct gatgaccaca
     8761 gatcatgttc gcgagttatt taatgtagca gaatcgtcaa cattgcattt tgctggcatc
     8821 aagctactgg aaataaatgc gacggctgat gtgcaccatc gcatcgaaat attacgcaac
     8881 ggcattcact ctctgaatga atctcgttaa acagaaccat ttatatatca ctttgaatat
     8941 ctaattagcc gttttctgat aattattacg aatattaaca acattagcgc ataaaaaata
     9001 ttgcataacc agaaggactc gttatgaaac ggatactcga aggcattctt gcgatcatca
     9061 tcgcaatact gtctt
//
LOCUS       DAZGJT010000148         8933 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZGJT010000148 DAZGJT010000000
VERSION     DAZGJT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8933
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.151"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1442)
                     /locus_tag="ACHKFK_004317"
     CDS             complement(<1..1442)
                     /locus_tag="ACHKFK_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054526.1"
                     /translation="MEKVSIRKINRTEHFPSLNLPSDTSWVVWWYGFLKKNKRRGEQP
                     KVAITFREVQADGTLCDRYYEQECLLTDLSFFKIGSVWTRGRCVAEILMEEKIFNVDF
                     SYANGGWKINSLYQAYKEGVSLPYDFSDYQLKSPYDKNWLLEFLTDDADKVIIHPLEF
                     FYRCYGHSDELKRVILTYSGNELRNRIFSEAYVEGDKIFYIKLRKRMNKYDAPFASFF
                     ALDGFTRKVIDRIQRQLDVCEDRKIYLKVAPWFKGKARIKVKGFWLENNVTFLGLSVT
                     GVSLPAGYTINYDRDNSNLVDEKAEQGSRQAWSNVPVRHIKNPDEPFSVDNAFSPDSG
                     ASTLEVEASVFEFLDDGCELSPLRRKKAKYISVTKNESDLHSLYSVNEVVDEQNSIST
                     GYMSLADKNEPERLYMESKGIVRDMWNAMVYLRGAYSERIKSLDWYTPESSFLNTQEP
                     ELVAIPEFNESEVENDKISKSILNWPYLDVV"
     gene            complement(1597..2328)
                     /locus_tag="ACHKFK_004318"
     CDS             complement(1597..2328)
                     /locus_tag="ACHKFK_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054527.1"
                     /translation="MPIKNKLGFISMKVVFNVMDGFDKTFLPLEFSGFHGRNGYCYLR
                     VQIKHGFIVFSCAQLLNYYRTSVTNAIEQVREAAVNALFREGVLSYTQQKEFLDILKT
                     SQRVDKEIDSQLWDYINANSIWFEYYNHNESLFLNDHFHIASFEGNRNPLWKKTSLED
                     LERAYPEFDFVIHKHHLEKWINGGLSPENVKKTIKEKGWSNKMLAARWGCTEVWVSKI
                     INDENRKVQWNDAINGLPVISDNMI"
     gene            complement(2460..3146)
                     /locus_tag="ACHKFK_004319"
     CDS             complement(2460..3146)
                     /locus_tag="ACHKFK_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HFO9054528.1"
                     /translation="MTTFIDHQKAFILLFNQTARYHKRHKVFEDFVSCSVIALENRLQ
                     FSEAREQKYLRIVSGYEKQDVINMAHLLAHVIEGLEQGFCDFLGSVFMQLELGDKYRS
                     QFFTPWGVACMMAQMQLGNVKALFENKPFITLSEPACGAGSMILAMADTLNRSGYPAY
                     RRMWVSETDIDPLAAGMAYIQLSLCGVAGEVVIGNSLCNERRRILLTPGHYLGNWSYR
                     LRHVQEQAAA"
     gene            complement(3269..4215)
                     /locus_tag="ACHKFK_004320"
                     /pseudo
     CDS             complement(3269..4215)
                     /locus_tag="ACHKFK_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            complement(4323..4760)
                     /locus_tag="ACHKFK_004321"
     CDS             complement(4323..4760)
                     /locus_tag="ACHKFK_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006859754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD29 domain-containing protein"
                     /protein_id="HFO9054529.1"
                     /translation="MMNVKSVTEVDDAVVARVSDVLEFAFPGQKFNVLKVCDSGVYNM
                     INVSWLDGPTEAEVRFITRAFEGKNGLRFVHESRKFSNEFVQECIDRLRKKYGQSNVP
                     PDVRVARYWKNDLWKIKTDRFPGNIDVAINEMGAETSKYRKVV"
     gene            complement(4834..5172)
                     /locus_tag="ACHKFK_004322"
     CDS             complement(4834..5172)
                     /locus_tag="ACHKFK_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054530.1"
                     /translation="MRPDLNILPGDSGCSVWFYNGMSQPRLLAGSIAGLLTDVTITSN
                     YRGDVTSEIHEVVQEWLATGRGNLADLKEELWYYNLYINPSADELMNASRRYGLGHTT
                     WLKDFISNAV"
     gene            complement(5240..5599)
                     /locus_tag="ACHKFK_004323"
     CDS             complement(5240..5599)
                     /locus_tag="ACHKFK_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054531.1"
                     /translation="MYSRFITGYWHLQSQSALLAHLCQLEGELAILRAWQRLGITPVP
                     EDEDEPSPLYSILWDVGEALGWLLYDLEMENVPAPGTIFHEVLDRVMSLGHETEHSIW
                     ADSISTGKRYAAIPDAF"
     gene            complement(5710..6693)
                     /locus_tag="ACHKFK_004324"
     CDS             complement(5710..6693)
                     /locus_tag="ACHKFK_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zincin-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HFO9054532.1"
                     /translation="MKTKKHTSCPVNKTPERDLYQLVTDRVIEAIEMGVAPWKRPWRS
                     VRTHSGDPMMPVNALTRSAYRGINVLLLWLAAEEAGYSSNCWLTYRQAQALGGQVRKG
                     EKSTMAVIYKSWHKQDEDENGNRIFDAEGNPVMVDVPVLKANPLFNVEQCDGLSLPEV
                     ATTVTENGANDTLNAVADRVLTMVNACGVKIHSLPQDRAYYTPVFDRIVLPLSSQFTS
                     AEDYWSTLLHELVHSTGHAKRLNREGIVSSGGSYGDERYAFEELIAEMGSAFLCAQLG
                     VTGNVQHENYIGAWLKILRADKKALFSACRQAREATEYLLSVHDTPAEKAA"
     gene            complement(6786..6923)
                     /locus_tag="ACHKFK_004325"
     CDS             complement(6786..6923)
                     /locus_tag="ACHKFK_004325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054533.1"
                     /translation="MTFPTPTERFFFYLLHRVPASVAWVSDTLKTFDFERHSLVMLAL
                     W"
     gene            complement(6901..7380)
                     /locus_tag="ACHKFK_004326"
     CDS             complement(6901..7380)
                     /locus_tag="ACHKFK_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054534.1"
                     /translation="MNSLLILLYVVVCRACTILRVIAPVSSVRAWCEYQLEGMTILRD
                     AYSSLAKAAGMQNDAFEYLESIARHLGYESGGDQLKSGLFRPFLWHIRAKGHRLGAVM
                     EMAVWQAWKNGCNDVCNPWEDVYNPWERGWNDGWTECLDQRIAALLKAVCIDFSDAD"
     gene            complement(7481..7861)
                     /locus_tag="ACHKFK_004327"
     CDS             complement(7481..7861)
                     /locus_tag="ACHKFK_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054535.1"
                     /translation="MGLDIFFLGRDRVTDAVVSVPETREVGYFRKVNPLIAWFETHCG
                     PVENAVERPVSRTELEALLSDLECLTPENCREFFPTTEGFFFGSQEYDQYYWKDVEIV
                     KSWVRSTLDSFDFERKILLLWAWW"
     gene            complement(8032..8433)
                     /locus_tag="ACHKFK_004328"
     CDS             complement(8032..8433)
                     /locus_tag="ACHKFK_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054536.1"
                     /translation="MFIPLLLRADMHITPFFHLLRQCLFNQKRCVHPQPPWSAPQLRH
                     LYCTPWFINATGNLSVRACIVSTNGALRHCFEFREGSHSREYRYWFEALPLSVVRFNV
                     FTGRISARRTLSAASRMAAQLTSFRYRFGLR"
BASE COUNT         2494 a         2365 c         1843 g         2231 t
ORIGIN      
        1 acaacatcaa ggtaaggcca gttaagaatc gacttggaaa ttttatcatt ttccacttcg
       61 ctttcattga actccgggat tgcaacaagt tcaggttctt gcgtatttaa gaatgagctt
      121 tctggggtat accagtccag agacttaatt ctttcagaat aagctcccct caaataaacc
      181 atagcattcc acatgtcacg aacaatccct tttgactcca tatacaatct ttcaggttca
      241 tttttatcag ctaatgacat atatcctgtg cttatgctat tttgctcatc aacaacttca
      301 tttaccgaat ataatgagtg taaatcactt tcattttttg taacggatat atattttgct
      361 ttttttctcc ttaaaggact cagttcacaa ccatcgtcaa gaaattcaaa aactgacgct
      421 tcaacttcaa gagtcgacgc tccagaatca ggagaaaaag cgttatcaac actgaaaggt
      481 tcatccggat tttttatatg ccgaactgga acattactcc aggcttgcct gctcccttgt
      541 tcagcttttt catcaaccag gttactgtta tctctgtcat aatttatagt atatcctgca
      601 ggcaaagaaa cacctgtaac agaaagccca aggaaagtaa cattattttc caaccaaaat
      661 cctttaactt ttattctggc ctttccttta aaccagggag ccactttgag gtatatttta
      721 cgatcttcac atacatctaa ttgacgttgt attctgtcaa tcactttccg ggtaaaccca
      781 tcaagggcaa agaaagaagc aaaaggagca tcatatttat tcattctctt tcttaatttt
      841 atataaaaga ttttgtctcc ctccacgtat gcctcagaaa aaatcctgtt acgcagttca
      901 tttcctgaat aagtaagaat aactctcttc aattcatcgg aatgtccata gcaacgataa
      961 aaaaactcaa gcggatggat gatcacctta tccgcatcat ctgttaaaaa ctcaaggagc
     1021 caatttttat catatggaga tttcagttga taatctgaaa aatcataagg taatgacaca
     1081 ccttctttat atgcctgata aagagaatta atcttccacc ctccatttgc ataagagaaa
     1141 tcaacattaa atattttttc ctccatcaga atttcagcaa cacaccttcc tcttgtccag
     1201 acactgccta ttttaaaaaa agacaaatca gtcaacagac attcttgctc ataatatcta
     1261 tcacaaagag ttccatcggc ctgaacttcc cgaaaggtaa ttgctacttt aggttgttca
     1321 cctcgtcttt tattcttttt taagaaacca taccaccaaa caacccagga tgtatcagaa
     1381 ggaagattta atgaaggaaa atgctctgtc ctgttgattt ttcttattga tactttttcc
     1441 atacatctgt ccctctaaac aacagaactc ttatctcaca agcaaatgtt gaagataaaa
     1501 tagttattca cttaaaccag aaacgctctg atgcgaacta catccagtat atattaataa
     1561 tcttcttgcc ttatgctggt gaagcatgat tacacatcaa atcatattat cacttatcac
     1621 aggaagccca ttaatagcat cattccactg aacctttcta ttttcatcgt taatgatttt
     1681 actcacccat acctctgtgc acccccagcg cgcagccaac atcttgttgc tccagccttt
     1741 ttcttttatt gtctttttta cattttcagg actaagtcct ccattaatcc atttttcaag
     1801 atgatgttta tgaataacaa agtcaaactc aggatatgct ctctctaaat cctccagtga
     1861 agttttcttc cacagtggat tcctgtttcc ctcaaaagag gcgatatgaa agtgatcatt
     1921 cagaaacaat gattcattat ggttatagta ttcaaaccat attgaattag catttatgta
     1981 atcccataat tgcgaatcaa tttctttatc tactcgttgt gatgttttta atatatccag
     2041 aaattctttt tgctgcgtgt atgacaatac cccctccctg aagagagcat taaccgcagc
     2101 ctctctgacc tgctcaattg catttgttac tgacgtacga taatagttca ataattgagc
     2161 gcatgagaaa acaataaatc catgtttaat ctgaaccctt aaataacagt acccattccg
     2221 gccatgaaaa ccagaaaact ccaggggcaa aaaagtctta tcaaaaccat ccattacatt
     2281 aaacacaacc ttcattgaaa taaaccccag tttatttttt attggcactt gcaatccttg
     2341 agtttaaaaa acaagagtaa cctgtcaaca ccacttacat aaccataaaa aaaataacaa
     2401 aaattgaccc acagatgtaa taacgccagt cctgagactg gcgtagtgat aaaaaatgat
     2461 caggccgctg cctgttcctg aacatggcgc agacgatatg accagtttcc gaggtaatgc
     2521 ccgggcgtca gcagaatccg gcgccgctca ttacacagcg agttaccaat caccacttct
     2581 ccggccacac cacacagcga caattgtata tacgccatac cagctgccag cgggtcaatg
     2641 tccgtttccg acacccacat cctgcggtat gccggatacc ccgaacggtt cagggtgtca
     2701 gccatagcca gaatcatact gccggcacca caggcaggtt cactgagcgt gataaagggt
     2761 ttgttctcaa acaacgcttt cacattcccc agctgcatct gtgccatcat gcaggccaca
     2821 ccccatggcg tgaaaaactg gctccggtat ttatccccca gctccagctg catgaacacg
     2881 ctgcccagaa agtcgcagaa tccctgctcc agtccctcaa tgacatgggc aagcaaatgc
     2941 gccatgttga taacatcctg cttttcataa ccactgacga ttcgcaagta cttctgctcc
     3001 cgtgcttcac tgaactgaag ccggttctcc agggcaatca cactgcagct gacaaagtcc
     3061 tcaaacacct tatgacgttt gtgataacgg gcagtctgat taaacagcaa aataaaagct
     3121 ttctgatggt cgataaaggt ggtcatgtgc ttctccataa aaaaacaggg aacacatggc
     3181 cccggggagg aacaagtgtt ccccttcggg ctgaaatgat agatgacagg aaaaaacggg
     3241 tgccgcgaat gtggcaccct gaggttagtt actggcctgc agccagcgga accggtaaag
     3301 gcgcggcttc tgtctgttct tcagcgcgct cctccaccag atacagccgg ggctgtggaa
     3361 gtttttccct ccccggccat tcggcctgcg ggtcactgtc cgtatgttca ccaccgtcat
     3421 aacagttgta accctgtatg ccccggacag gttcgctgta ccagtgccgt atctcacagt
     3481 cgaacacctt cgagatttca ccaaccagct cacctgaagg cggataccag ggcgtgtctg
     3541 tcagcagcac cagactgctc atctcatcac gcacccagcg gacattgtga cctgatggcc
     3601 actccacacc gtatatccgg ctgtaaaggc tggtcgtggt ggaaatgccc tgcagcagtc
     3661 cacctgaacc gttcagctct gtggccagac ggggggtatg accatcagca tgtcacaggg
     3721 ctgcgcctgt tccgggtaat ccgacagccg gctccagcag gcttcggcat caatgtgttc
     3781 actcagtccg accagaccaa accagtcaga atactgacaa tgcattaacg tggtgattat
     3841 ctcccgggca ttatccggca gattccccca gcggactgcg ccaatacatg actggtgata
     3901 aaggcggtca atggtcttta tcgtctcttc atccagtacg gcatctttta ccagcagccc
     3961 cagccactgt tcaaatgcct ggttagccgg agatgacatc cccgtaccat gacgaaccag
     4021 cgccggatat ggcggatact gcgtcgtccg ggtaggtttc accagtccgg cacaaccgat
     4081 cagaaacagc tgtatactct gctgtatggc atggcggtaa cgcggtgttt cacatccttc
     4141 aatccactgc gtcagcacat ccaggcagac tgacttaccg gtaatttcaa aacgatttct
     4201 gcaccattcg gccatattca atgctcctct gataaatgaa gagggcattg tcccgtgagg
     4261 tacaatgccc tcacgggaaa agcggagttg tcacactccg ccatcgtcaa aagtccgtcc
     4321 ggttatacaa ccttccggta tttactggtt tctgccccca tttcgttgat agctacgtcg
     4381 atattgcccg ggaaacgatc tgttttaatc ttccacaaat cgtttttcca gtaacgtgcc
     4441 actctcacat ctggcggaac gttgctctgc ccgtactttt tacgaagccg gtcaatgcac
     4501 tcctgcacaa attcattact gaatttgcgg gattcgtgca cgaacctcag cccgtttttg
     4561 ccttcaaacg cacgggtaat aaaccgcact tcagcctctg ttggtccatc cagccaggac
     4621 acattaatca tgttgtaaac gccggaatcg cataccttca gcacgttaaa tttctgcccc
     4681 ggaaatgcga attccagaac gtcactgaca cgggcaacca cggcatcgtc aacctcagtc
     4741 acagacttaa cattcatcat tctgctcctc tgtatgaaaa tcactgaggg aacagccccg
     4801 tgaggagtcg ggccctcacg ggatggcggt gtgtcacacc gcgttactga taaagtcttt
     4861 cagccaggtg gtatgcccga ggccataccg cctgctggca ttcataagtt cgtctgccga
     4921 cggatttatg taaaggttgt agtaccagag ttcttccttt aaatccgcca ggttacccct
     4981 gccggtcgca agccactcct gcaccacttc atgaatttca gatgtgacgt caccgcggta
     5041 attcgatgtg atcgtaacat ccgtcagtaa cccggcaata ctgcccgcca gcagacgcgg
     5101 ttgagacatg ccgttataga accatacgct gcagccggaa tctccgggta aaatattcaa
     5161 atcgggtctc atgaaatttc tcctgaaaaa gcagggaccc gtcccacagg gatgagtccc
     5221 tgtggggatg taaaaaccgt taaaatgcat caggtattgc agcgtaccgc ttcccggtac
     5281 tgatactgtc agcccagata ctgtgctctg tttcatgtcc cagagacata accctgtcga
     5341 gtacctcgtg gaaaatcgtt cccggtgcag gaacattttc catctccagg tcatacagca
     5401 accagcccag agcctcacca acatcccaca aaatcgagta aagtggtgaa ggctcatcct
     5461 catcctccgg caccggggta attccgagac gttgccaggc gcgaagtatc gccagttcac
     5521 cttccagctg acagagatgt gccagcaaag cggactgact ctgaagatgc cagtaaccag
     5581 taataaaacg cgaatacata aaaccccctg tctgattctt tctgatgaaa gagcacaccg
     5641 tccgaaggaa cgatgccctc cgggcggtga acaacagagt tcagattgtt cacccgcgat
     5701 tgttaagcat tacgccgctt tttctgccgg cgtatcgtgt acggaaagaa ggtattccgt
     5761 tgcctctctg gcctgtcgac atgcactgaa cagcgctttt ttgtcagcac gaagaatttt
     5821 cagccatgcg ccaatataat tctcatgctg cacatttccg gtgacaccca gttgagcgca
     5881 cagaaaggca ctgcccattt cggcgataag ctcttcgaac gcataacgtt catcgccata
     5941 gctcccaccg gaggaaacta tcccttcccg gttaagacgt ttcgcatgac cggtactgtg
     6001 taccagctca tgcagtaatg tggaccagta atcttccgct gaagtgaact gtgacgagag
     6061 cggcaggacg atacgatcaa ataccggggt gtaatacgcc cggtcctgag gtaaagaatg
     6121 aatcttcaca ccgcaggcat tcaccatcgt cagtacacga tcagccacag cattcagcgt
     6181 gtcgttcgca ccgttctcag tgacggttgt cgccacctcc ggcagcgaca atccatcaca
     6241 ctgttcaacg ttaaagagag gattcgcttt cagcaccggt acatcaacca taacggggtt
     6301 accctctgcg tcgaatatcc ggttgccgtt ctcatcctca tcctgcttat gccagctttt
     6361 gtatatcacg gccatcgtgg acttctcgcc cttacggacc tgccccccca gcgcctgagc
     6421 ctgacggtac gtcaaccagc agttactgct gtaaccggct tcttctgcgg cgagccacaa
     6481 cagcaacaca ttgatcccac ggtaagcgct gcgtgtcagt gcattaaccg gcatcattgg
     6541 atcgccgctg tgagtccgta ctgagcgcca tggacgtttc cagggggcta cgcccatttc
     6601 gatggcctca atcaccctgt ccgtcaccaa ctgatacagg tcacgctccg gggttttgtt
     6661 cacgggacat gaagtatgtt ttttcgtttt cataagagca cgctccataa aaaagatgca
     6721 tgctccccgt acggtgaaca tgcaccatac ggttaaatta ataacgaaaa tcagactggc
     6781 cggacttacc acaacgccag catcaccagt gaatgacgct caaaatcaaa cgtcttcagc
     6841 gtatcgctga cccacgccac cgacgcaggt acacggtgca gtaagtaaaa gaaaaaccgt
     6901 tcagtcggcg tcggaaaagt caatacagac cgccttcagg agagcggcta tacgttgatc
     6961 cagacattca gtccagccat cgttccatcc acgctcccat ggattgtaga catcctccca
     7021 tggattgcag acatcgttac agccattctt ccatgcctgc cataccgcca tttccatgac
     7081 agctcccagc ctgtggcctt tcgcacgtat atgccacaga aacggccgga aaagtcctga
     7141 tttcagttgg tcaccaccgc tttcgtagcc cagatgacga gcaatacttt caagatattc
     7201 aaatgcatca ttctgcattc cagccgcctt agccagcgag ctgtacgcat cacgaagtat
     7261 cgtcatacct tccagttgat actcacacca cgcacggaca gacgacactg gtgcgattac
     7321 ccgaagaatg gtgcaggcac ggcatacaac aacgtaaagg agtattaaca gactattcat
     7381 gtgttcacct cagatacaaa aaatcacggg aacacacctg ccctacggga cacatgttcc
     7441 cgacagggta ataaggttaa aaacaaggac agaaaaataa ttaccaccat gcccagagca
     7501 acagtatttt tcgttcaaaa tcaaacgaat caagcgtgct tctgacccac gatttcacga
     7561 tctcaacatc cttccagtaa tactggtcat actcctgaga accaaaaaag aatccttctg
     7621 ttgtggggaa gaattcccgg cagttctccg gcgtaaggca ttcaagatcg ctcagtagtg
     7681 cctccagttc cgttctggaa accggacgct ccacggcatt ttcaaccggt ccacaatgcg
     7741 tctcaaacca ggcaatcagt gggttaacct tgcgaaagta acccacttcc cgcgtttccg
     7801 gtaccgatac taccgcgtcg gtaacacgat cacgcccaag gaaaaaaata tcgagtccca
     7861 taaacaatct cctgataaaa acagcgggac cgccccccga acgggagcgg tcccggacgg
     7921 gttaaggaaa actcagattt gtcatcgcgc acgcgtggta cggtcagtca gatccgggcg
     7981 ttgtgtgata aacctttcaa tggtcatgac gaccgttcaa caggcttggc ttcagcgaag
     8041 accgaagcga tagcgaaaag aagtaagctg cgcggccata cgggaggcag cagagagcgt
     8101 gcggcgagcg gatattcgac cggtaaagac attaaaccgc acgacagaca gcggaagtgc
     8161 ctcaaaccag taacgatatt cacggctgtg agagccttca cggaactcaa agcagtgtcg
     8221 caatgcaccg ttggtggaaa caatgcaggc tctgactgac agattgccgg tggcgttaat
     8281 aaaccacggt gtgcagtaca gatgcctcag ttgtggagcg gaccagggcg gttgtgggtg
     8341 aacacaacgc ttctgattaa acagacattg tctgagaaga tgaaagaaag gcgttatgtg
     8401 catatctgca cgtaacagaa gtggtataaa catcggaacc tccgaatgaa aaagacggac
     8461 attccgccct gacggactgg aaaatccgtc agggtatgcc agcagaacac ctggcacagt
     8521 caagttgtca taaatgcaac cggaaaaacc ggctggaata tcaacgtacc tcaatcacca
     8581 gtagtgatat ccgtttccag aggctaaacg gatgtaccgc cataaaggca tcctctttca
     8641 ggctacgagg atattgcaat cacccgaagg cgtccctctc agatcaccgg gacattggat
     8701 ggtcgccgga gcgacagtta atgcagatgc aggttaacaa aaagggaaat ctgccgtcaa
     8761 ccatatgaac atgttgattt cattatgaat gtgttgtttt aatataaatt aatcggtaac
     8821 gtcataaaaa gaatataaga aacagataaa gcatgcattt acacacatat cagtataatg
     8881 ataaattacc tctccaaaaa cacgacaaag gtataattat ggaagtttcc gtt
//
LOCUS       DAZGJT010000149         8795 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZGJT010000149 DAZGJT010000000
VERSION     DAZGJT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.152"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..354
                     /locus_tag="ACHKFK_004329"
                     /pseudo
     CDS             1..354
                     /locus_tag="ACHKFK_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232088.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(370..446)
                     /locus_tag="ACHKFK_004330"
     tRNA            complement(370..446)
                     /locus_tag="ACHKFK_004330"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(410..412),aa:Arg,seq:tct)
     gene            complement(545..676)
                     /locus_tag="ACHKFK_004331"
     CDS             complement(545..676)
                     /locus_tag="ACHKFK_004331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054537.1"
                     /translation="MSILSEAWRAVSFRFLKSKTFCTWLIHTYHDNRRWFAAIVSYI"
     gene            717..1583
                     /gene="folD"
                     /locus_tag="ACHKFK_004332"
     CDS             717..1583
                     /gene="folD"
                     /locus_tag="ACHKFK_004332"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO9054538.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1585..1797
                     /gene="ybcJ"
                     /locus_tag="ACHKFK_004333"
     CDS             1585..1797
                     /gene="ybcJ"
                     /locus_tag="ACHKFK_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO9054539.1"
                     /translation="MATFSLGKHPHVEQCDLLKLEGWSESGAQAKIAIAEGLVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1905..2414
                     /gene="ybcI"
                     /locus_tag="ACHKFK_004334"
     CDS             1905..2414
                     /gene="ybcI"
                     /locus_tag="ACHKFK_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO9054540.1"
                     /translation="MPTVITHAAVPLSIGLGLGSKAIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMLLMGMLW"
     gene            complement(2462..3847)
                     /gene="cysS"
                     /locus_tag="ACHKFK_004335"
     CDS             complement(2462..3847)
                     /gene="cysS"
                     /locus_tag="ACHKFK_004335"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO9054541.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            4021..4515
                     /gene="ppiB"
                     /locus_tag="ACHKFK_004336"
     CDS             4021..4515
                     /gene="ppiB"
                     /locus_tag="ACHKFK_004336"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO9054542.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKDPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4518..5240
                     /gene="lpxH"
                     /locus_tag="ACHKFK_004337"
     CDS             4518..5240
                     /gene="lpxH"
                     /locus_tag="ACHKFK_004337"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO9054543.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHHQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQTAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5351..5680
                     /locus_tag="ACHKFK_004338"
     CDS             5351..5680
                     /locus_tag="ACHKFK_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054544.1"
                     /translation="MQRCNGFLISGIALTAGAPLCISWFGLVGFYFGALSLVAAAVIS
                     WIVYFWLIYLVLTRPHNQRFVERFILIVGICFFPWSIVLIWAFFASSARAHNQHAEQI
                     KNSTTDG"
     gene            5776..6285
                     /gene="purE"
                     /locus_tag="ACHKFK_004339"
     CDS             5776..6285
                     /gene="purE"
                     /locus_tag="ACHKFK_004339"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO9054545.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNEWRKAQTDEVL
                     ENPDPRGAA"
     gene            6282..7349
                     /gene="purK"
                     /locus_tag="ACHKFK_004340"
     CDS             6282..7349
                     /gene="purK"
                     /locus_tag="ACHKFK_004340"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO9054546.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLSE
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRADETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQLQAQAEEMLSAIMQELS
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDLNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            7481..8784
                     /locus_tag="ACHKFK_004341"
     CDS             join(7481..7916,7916..8784)
                     /locus_tag="ACHKFK_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558234.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1397 family transposase"
                     /protein_id="HFO9054547.1"
                     /translation="MAGHAGIISTAKLFQLSHTSLSHWINLFLLHGPRALDCRHKRSY
                     SPEDKLCVVLYALGHSESLPRVAARFNIPSHNTVKNWIKGYRKSGNEAFIRRRKEKSM
                     TRSDDTHENEANMTPEEMKNELRYLRAENAYLKAMQEHLLEKKPPGAGEKTKVTQSLR
                     YGHCQSDLLKAAGLARSTLYYQLSLQKAKDKYADVKQLIASIFHEHRGCYGYRRIHCE
                     LQKRGLKFSGKTVRKLMQQLGLKSPVRLKKYRSYRGNMGLAAENILQRQFKAEAPCEK
                     WVTDITEFRAGGQKLYLSPILDLFNGEIVAWETACRPTEELVKRMLNKGLESLAEGEK
                     PLLHSDQGWHYRIKSYQSALADRGLVQSMSRKGNCLDNAVMENFFGHLKEEMYYRRDY
                     RNVEELENAVNEYITYWNQKRIKLSPGGLSPVEYRTEYQKAG"
BASE COUNT         2121 a         2242 c         2340 g         2092 t
ORIGIN      
        1 tcacaaatgt gggtgtacat accagggcgg ctaagcgagc agatggaaca tcaatgcctg
       61 cgctcaggaa gatgcgcatt gacagcaaga cagcatggct atcagcttgt cgtcgtgcag
      121 gagttgaaaa tttccgtttc catgacctct ggcatacccg ggcaagcagg ttgattccgt
      181 caggcgtccc attatcagtg cttcaggaaa tgggcggatg ggagtccaga gaaatggttc
      241 gtagatatgc tcaccttgcg cctaatcatt tgactgagca cgcgaggaaa atagacgaca
      301 tttttggtga taatgtccca ctctggaatt atagaggaaa taaagaaggc gtaactgatt
      361 gaattttaat ggcgcgccct gcaggattcg aacctgcggc ccacgactta gaaggtcgtt
      421 gctctatcca actgagctaa gggcgcattg ataccacggt gtgctgtaat cgcgtgaatt
      481 atacggtcaa cccttgctga gtcaatggct tttgaaccag ttgctgaaca agtgtacgac
      541 cgcatcagat gtagctcact atggcggcaa accaacgacg gttatcatga taggtgtgaa
      601 tcagccaggt acagaaggtt ttacttttca gaaaacgaaa actgacagcg cgccacgctt
      661 ctgacaaaat agacacatcc ccttcgacct acgtaacaga tggaatcctc tctctgatgg
      721 cagcaaagat tattgacggt aaaacgattg cgcagcaggt gcgctctgaa gttgctcaaa
      781 aagttcaggc gcgtattgca gccggactgc gggcgccagg actggccgtt gtgctggtgg
      841 gtagcaaccc tgcatcgcaa atttatgtcg caagcaaacg caaggcttgt gaagaagtcg
      901 ggttcgtctc ccgctcttat gacctcccgg aaaccaccag cgaagcggag ctgctggaac
      961 ttatcgatgc gctgaatgcc gacaacacca ttgatggcat tctggttcaa ctgccgttac
     1021 cggcgggtat tgataacgtc aaagtgctgg aacgtattca tccggacaaa gacgtggacg
     1081 gtttccatcc ttacaacgtc ggtcgtctgt gccagcgcgc gccacgtctg cgtccctgta
     1141 ccccgcgcgg tatcgtcacg ctgcttgagc gttacaacat tgataccttc ggccttaacg
     1201 ccgtggtgat tggcgcatcg aatatcgtcg gtcgcccaat gagcatggaa ctgctgctgg
     1261 caggttgcac caccaccgtg actcaccgtt tcactaaaaa tctgcgtcac cacgtagaaa
     1321 atgccgattt gttgatcgtt gccgtcggca agccgggctt tattcctggt gactggatca
     1381 aagaaggcgc aattgtgatt gatgtcggca tcaaccgtct ggaaaatggc aaagttgtgg
     1441 gcgacgtcgt gtttgaagac gcggctaaac gcgcctcata cattacgcct gttccgggcg
     1501 gcgttggccc gatgacggtt gccacgctga ttgaaaacac gctacaggca tgcgttgaat
     1561 atcatgatcc acagggtgag taacatggcg acattttctt taggtaaaca tccgcacgtt
     1621 gagcagtgcg acttgctgaa actggaaggc tggagcgaaa gcggcgcgca ggcgaaaatc
     1681 gcgatagccg aaggcctggt gaaagtcgac ggtgcggttg aaacgcgcaa acgctgcaaa
     1741 atcgtcgccg gtcagacagt gagttttgca ggtcacagcg tacaggttgt tgcctgatcc
     1801 ctttcccggc ctgtatcaac aggccggttc tcttttcaga cttttgccct ctacgccccg
     1861 ttcatccatg ctgatagcat cgacattttg ccgaagcgac catcatgcca accgttatta
     1921 cgcacgccgc cgttccccta agcattggtt tagggttggg ttcgaaagcc atccctccgc
     1981 gtctgttatt tgccggaatc atcctggcga tgctgcccga tgccgatgta ttgtcgttta
     2041 aatttggcgt tgcttacgga aatgtttttg gtcatcgcgg gtttacccat tcgctggtgt
     2101 ttgcgtttgt tgtcccgctg ttatgtgtgc tgattggacg tcgatggttc agggctgggc
     2161 tgattcgctg ctggctgttt ttaaccgtct cgttgctatc gcacagcttg ctggattcgg
     2221 taaccactgg cggtaaaggg gttggctggc tgtggccgtg gtcagatgaa cgctttttcg
     2281 caccctggca ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acgccgtacg
     2341 ggcatcaggt gattatttct gaattgatgt gggtgtggtt gccggggatg ttactgatgg
     2401 gaatgttgtg gtagcgcaga cgataaccgg atgcgaaaac tcgcatccgg caatagcgca
     2461 attacttacg acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt
     2521 taagacggtc acgcgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac
     2581 gctgttgaat taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcaccgcttt
     2641 gcaggaacgc ttccggttct tgctccagca ggcccaatac ggcagaaagt ttacgcaggt
     2701 gagacgccat tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg
     2761 ccatatcaaa cagcacggaa taggcttccg gggtgttgaa atcgtcatcc attgcttcaa
     2821 taaagcgcgc ttcaaacgct tcgccaccgg caggcgcaac ggttttgtct gtgccgcgca
     2881 aggcagtata gagacgttcc agcgccgcac gcgcctgttt caggttctct tcactgtagt
     2941 tcaactgact gcgatagtga cccgacatca ggaagtaacg cacagtttcc gcatcgtagt
     3001 atttcagcac gtcacgcacg gtgaagaagt tacccagcga tttagacatc ttctcgcggt
     3061 caaccatcac cataccggag tgcatccagt aattcacata ctggccatcg tgcgcacagg
     3121 tggactgggc aatttcgttc tcgtggtgcg ggaacatcag gtcagaaccg ccgccgtgga
     3181 tatcaaagtg gttacccaac tgtttgcagt tcattgccga acactcaatg tgccagccag
     3241 gacggcccgc gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca
     3301 gaacgaagtc catcgggttg cgtttgtcgt cgaccacgtc aacgcgcgcg cctgcctgca
     3361 gctgatcgag atcctgacgc gacagcacgc catatgtcgg atcggtcggg acgtcgaaca
     3421 tcacgtcgcc gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt
     3481 caataatttc ggcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca
     3541 aagcatcaaa atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt
     3601 cgccattttc attggcgcgt ttgatgattt tgtcgtcgat atcagtaatg ttgcgcacat
     3661 acttcagctt atagccgagg aaacgcagat agcgcgcaac cacgtcaaag gcaacaaagg
     3721 tacgcccgtg accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga
     3781 cttccccggc gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt
     3841 ttagcatcga agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata
     3901 tttcccgcgc gaatcagcat acattgcgag atgatccgat cgtgcggtta tgctataaca
     3961 ccaccctata tatgacccga accgggttga agcaccaatc aaacggaaca ggatgcaaaa
     4021 atggttactt tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca
     4081 cctgaaacag ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt
     4141 ttccaccgtg ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa
     4201 caaaaagcca ccaaagaccc gatcaaaaac gaagcgaaca acggtctgaa aaatacccgt
     4261 ggtacgctgg caatggcccg tactcaggca ccgcactctg caactgccca gttcttcatc
     4321 aacgtggttg ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac
     4381 tgcgtatttg ctgaagtggt tgaaggcatg gacgtggtgg acaaaatcaa aggtgtggca
     4441 accggtcgta gcggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg
     4501 accgttagcg agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag
     4561 aaccggcgat caccgccggt tttctgcgtt ttttagcggg ggaggcccgc aaggccgatg
     4621 cgctgtatat tcttggcgat ctgtttgaag cctggatcgg cgacgacgat cccaacccac
     4681 ttcatcacca gatggccgca gcgatcaaag cggtatccga ttccggcgtt ccctgttatt
     4741 tcattcatgg caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga
     4801 cgttattgcc ggaagaaaag gtgctcgaac tttatggtcg tcgggtgttg attatgcatg
     4861 gcgacacgct gtgcaccgat gacgcgggtt atcaggcttt tcgtgccaaa gtccacaaac
     4921 cctggctgca aacgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc
     4981 gaatgcgcgc gaacagtaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta
     5041 accaaaacgc ggtggtcagt gcgatggaaa aacatcaagt gcaatggctg atccacgggc
     5101 atacccatcg cccggcggtg catgaactta tcgccaatca gcaaacggct tttcgcgtgg
     5161 tactgggtgc ctggcataca gaaggttcga tggtgaaagt cacggcggat gacgttgagc
     5221 taatccattt tcctttctga ccacgaatgg acagattctg aaaaagcacc gaactaacgc
     5281 aacctgtata tcttttttaa aatcagcaag gtaataatct ctgagtgaaa taacaacagg
     5341 gaatattatt atgcaacgct gcaacggatt tttgatttct ggcatcgccc ttacggcagg
     5401 agcgccactg tgtattagct ggttcggtct tgtcggcttc tattttggcg cattgtcact
     5461 ggtagctgcg gcggtcattt cctggatagt ctatttctgg ctcatttatt tagtactcac
     5521 ccgcccccat aaccaacgtt ttgtagaaag atttatattg attgtcggta tttgcttttt
     5581 tccatggagc attgtgttga tatgggcatt ctttgccagt tcagccagag cgcataatca
     5641 acacgcagaa caaattaaaa actcaacaac tgacggttaa ttccgcctac gcaaccgttt
     5701 tccttgcact ctttccgtgc tattctctgt gccctctaaa gccgagagtt gtgcaccaca
     5761 ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc gtgtcgccat cgtgatgggg
     5821 tccaaaagcg actgggctac catgcagttc gccgccgaaa tcttcgaaat cctgaatgtc
     5881 ccgcaccacg ttgaagtggt ttctgctcac cgcacccccg ataaactgtt cagcttcgcc
     5941 gaaagcgccg aagagaacgg ttatcaggtg attattgcgg gcgcaggcgg cgcagcgcac
     6001 ctgcctggca tgattgccgc caaaacgctg gtgccggtgc tgggcgtgcc agtacagagc
     6061 gccgcactga gcggtgtcga tagcctctac tccatcgtac aaatgccgcg cggcattccg
     6121 gtgggtacgc tggcgattgg taaagctggc gcggcaaacg cggcgttact ggcagcacaa
     6181 attcttgcga ctcatgataa agaactgcac cagcgtctga atgaatggcg caaagcacaa
     6241 actgatgaag tgctggaaaa cccggacccg cgaggtgcgg catgaaacag gtttgcgtcc
     6301 tcggtaacgg gcagttaggc cgtatgctgc gtcaggcagg cgaaccgtta ggcattgctg
     6361 tctggccggt cgggctggac gctgaaccgg cggcggtgcc ttttcaacaa agcgtgatta
     6421 ccgctgagat cgaacgctgg ccggaaactg cattaacccg cgagctggcg cgccatccgg
     6481 cctttgtgaa ccgcgatgtg ttcccgatta ttgccgaccg tctgactcag aagcagcttt
     6541 tcgataagct ccacctgccg accgcaccgt ggcagttact ttccgaacgc agcgagtggc
     6601 ctgcggtgtt tgatcgttta ggtgagctgg cgattgttaa gcgccgcact ggtggctatg
     6661 acggacgcgg tcaatggcgt ttacgcgccg atgaaaccga acagttacct gctgaatgct
     6721 acggcgaatg tattgtcgag cagggcataa acttctctgg tgaagtgtcg ctggttggcg
     6781 cgcgcggttt tgatggcagc accgtgtttt atccgctgac gcataacctg catcaggacg
     6841 gcattttgcg caccagcgtc gctttcccgc aggccaacgc acagctgcag gcgcaagctg
     6901 aagagatgtt gtcggcgatt atgcaggagc tgagctatgt aggcgtgatg gcaatggagt
     6961 gttttgtcac cccacaaggt ctgctgatca acgaactggc tccgcgtgtg cataacagcg
     7021 gtcactggac gcaaaacggt gccagcatca gccagtttga gctacatctg cgggcgatta
     7081 ccgatctgcc gttaccgcaa ccagtagtga ataatccgtc ggtgatgatc aatctgattg
     7141 gtagcgatct gaattatgac tggctgaaac tgccgcttgt gcatctgcac tggtacgaca
     7201 aagaagtccg tccgggccgt aaagtggggc atttgaattt gaccgacagc gacacatcgc
     7261 gtctgaccgc gacgctggaa gccttaatcc cactgctgcc gccggagtat gccagcggcg
     7321 tgatgtgggc gcagagcaag ttcagttaat tgatgctcat tttcttgccg gatgcggcgt
     7381 aaactgaact gcaccccaaa tgttggacat aatctgacat ctgaaggtgc agtttatgaa
     7441 acattaattt gaagtaaaac ttgccgctgt aaatcattac ctggctggtc atgcaggcat
     7501 catttctacg gcaaaactct ttcaactttc tcacaccagc ctctcacact ggattaatct
     7561 ttttcttctt cacggtcctc gggcactgga ttgcagacac aagcgtagct attctcctga
     7621 agataaactt tgcgtggttc tttatgctct cgggcattct gagtctctac cccgggtagc
     7681 tgcccggttt aacattccca gccacaacac ggttaaaaac tggataaaag gctaccgcaa
     7741 atctggtaat gaggccttta tcaggcgcag gaaggagaaa agcatgaccc gttctgatga
     7801 tacccatgaa aacgaggcaa acatgacgcc ggaagagatg aagaatgagc tgcgttatct
     7861 tcgcgcagaa aatgcatatc tgaaagccat gcaggagcat cttctggaaa aaaagcgcca
     7921 ggagctggag aaaaaacgaa agtcacccag agcctgaggt acggacactg ccagtctgac
     7981 ctgttaaagg ccgcagggct ggccagaagc acgctgtact accagctgtc actgcagaag
     8041 gctaaggaca agtatgctga tgtgaaacag ctcatagcct ccatcttcca tgaacaccgg
     8101 ggatgttatg ggtaccggcg gatccattgt gagcttcaga aaagaggtct taagttcagt
     8161 ggcaaaacgg tgcgaaaact gatgcaacag ctcggcctga agtctcctgt gcgcctgaag
     8221 aaataccggt cttaccgggg aaatatggga ctcgctgcag aaaatatcct tcagcgacag
     8281 ttcaaagcgg aagctccctg tgagaaatgg gtgacagata tcaccgaatt cagggctggc
     8341 ggacaaaaac tgtacctgtc cccgatactt gatttgttta acggggaaat cgtggcgtgg
     8401 gaaacggcct gcaggcctac agaggagctg gtgaagcgga tgttaaataa ggggctggaa
     8461 agccttgctg aaggggagaa accgctgctc cacagtgatc aaggatggca ttaccggata
     8521 aaaagttatc agtccgccct ggcggacaga gggttagtgc agagcatgtc gcgcaagggc
     8581 aactgccttg ataatgcggt aatggagaac ttctttggtc acctgaaaga agaaatgtac
     8641 tatcgccggg actacagaaa tgtagaagag ctggaaaatg ccgttaacga atacataact
     8701 tactggaatc aaaaaagaat aaaactcagt cctgggggac tcagcccggt agaataccgg
     8761 actgagtatc aaaaagccgg ttaactgaaa ctgtc
//
LOCUS       DAZGJT010000150         8687 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZGJT010000150 DAZGJT010000000
VERSION     DAZGJT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.153"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(107..727)
                     /gene="entD"
                     /locus_tag="ACHKFK_004342"
     CDS             complement(107..727)
                     /gene="entD"
                     /locus_tag="ACHKFK_004342"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO9054548.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPASFREQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAGVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLAECGLTFSLALTLAFSAKESAFKACER
                     QTDADFLDYQIISRNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(902..3142)
                     /gene="fepA"
                     /locus_tag="ACHKFK_004343"
     CDS             complement(902..3142)
                     /gene="fepA"
                     /locus_tag="ACHKFK_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO9054549.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKAAQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     KNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            3385..4587
                     /gene="fes"
                     /locus_tag="ACHKFK_004344"
     CDS             3385..4587
                     /gene="fes"
                     /locus_tag="ACHKFK_004344"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO9054550.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEGHS
                     TIKRVWVYITGMTDHHQNSQPRSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPALTSL
                     THRRQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPQVKTIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGHQEGVLIEQLKTGEVSAEG
                     LRIVLEAGVREPMIMQANQALCAQLHPLKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            4590..4808
                     /gene="ybdZ"
                     /locus_tag="ACHKFK_004345"
     CDS             4590..4808
                     /gene="ybdZ"
                     /locus_tag="ACHKFK_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO9054551.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDVVCQPQ
                     SQASCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            4805..8686
                     /gene="entF"
                     /locus_tag="ACHKFK_004346"
     CDS             4805..8686
                     /gene="entF"
                     /locus_tag="ACHKFK_004346"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO9054552.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDAQTFELPEIIDLRTNIDPHGTARALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPQLATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQAQTHPLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQI
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTRLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCSTLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAEVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQTPGRVP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSTQRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DLQWSIIGIQSPRPHGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVAFDGKATLFVAERTLQEGMSPEQAWAPWIAEL
                     DIYRQDCAHVDIISPVAFEKIGPIIRATLNK"
BASE COUNT         1882 a         2331 c         2387 g         2087 t
ORIGIN      
        1 taatccccgc atatccggtt gtcaggtcag gatggtaagg cacctgcttt acactttcgc
       61 ccgcagtcag agatggctgc gggcgaatcg taccagatgt tgtcaattaa tcgtgttggc
      121 acagcgttat gactattttt tcttttatct gccagtgcac agcaaacatc tcattctcac
      181 gatgaattat gacctgttgt ttattccggc taattatctg atagtccaga aaatctgcat
      241 cagtttgtct ttcacatgcc ttaaatgcgc tctctttggc ggaaaatgcc agtgtcagcg
      301 ccagagaaaa ggttaaaccg cattctgcga gtcgctcgtg ttctgctggt gtaataatgt
      361 tgtctgtcaa ttctcttgcg gtttgtacag agaaaatctc ttcgatatca atgccaatcg
      421 gttgacgaga taccacggct aatgccgtag tcccacagtg actaatgctg ccgtataccc
      481 ccgcaggcca cacaggttgg cgtagctcgc cgattgcagg cacacattta tagccatatt
      541 cccgcagcgc ataaattgca gcgatccgcc cggctaaatg ctctgtttta cgtttacgtc
      601 cagcgtgttg aagttgtgcg tagtgcggca gccagagtaa atcctgctcg cgaaaactcg
      661 ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa ggggagggag gtatgcgtag
      721 ttttcatgtc aaccatatta gagaatgatg acaaacgcaa aactgcctga tgcgctacgc
      781 ttatcaggcc tacgtggtcc tgcaatatat tgaattggca agatttttgt aggccggata
      841 aggcgttcac gccgcatccg gcatgaacta cgcgcacttt gtcaacaatc tgacgttagc
      901 ttcagaagtg agtattaatg ctcatatacc acgtacgtcc cggctcgtta taggtatacg
      961 ccccggcacc tgcgatatag ttggctcctg ccagatcgcc cgtggtctgg gcattacccg
     1021 cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc ggtcagactg acattcttgg
     1081 tcacatccca ggtcgcgctc aggccaacaa tgctgtacgg actgatttct ttggtttccg
     1141 gtccaaccgc tggctgacct ttatagttgt acttcttcgg ctgctgtttg ccgtaccagg
     1201 tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca gctcagcgtt gagttcaacg
     1261 tatactctgg gatgatcgac aaacggtcgc ccgtggtttt gttttcactc ttcagcatat
     1321 aagtgatgtt attggtccac atcaccgttt cgctaaccgg tacgtttaac gatccttcca
     1381 gaccttcaac caccgctttc ggcacgttat cccactgata gagatcggtg ccgactgcgt
     1441 ttttccctac agctacatag cccgcttcaa tcttattgcg ataatcgtta cggaaccagg
     1501 tcacgcccgc cagccaaccg tcgcgtttga actccagacc aatctccttg ttgatgctgg
     1561 tttctgcttt caggtcatcg ttaccttgca gatagcagcc gcccgcgctg gcatagcagc
     1621 cctgaccttt actgtagaga atgtagtttg ggttagtctg gtacaggctc ggcgctttat
     1681 aggcgcgggc gatgcccatt ttcagcgtga agtcatcgcc taaaccttgc gaaatgttca
     1741 gcgccgggct ccagttattg ccgacaatac tgtgatgatc gaaacgcagc cccggcgtta
     1801 cgatggtgct gtcagtcagc tccatattgt tttcggcaaa cagcgagaaa atttctgctt
     1861 ttgaatacgg gctacggtcg gtggcactca cgccatcaat agcaccgccg gtattcgttc
     1921 cggttagtgc ctgggtgttg gaactcaagt ccttcatccg ttgctgattc cactctgtac
     1981 ccagcgtcag cgtttggttt acgaggaaat caatcggcag gttaacttcg ctgtgcagca
     2041 tcacgtcatc aagatcgata tcgacaaaat cctgtgccgc tttttcgtta aatttccctt
     2101 cggtaccgcc cgccagacct tccggaatac gcgagttacg ggtgtgttcg tactgcaccc
     2161 agttgctggt ggtcacgccg ttatcccagc cgccgttcca ggtcagcgag tagttctggc
     2221 gatacaggcg gttggtttca tcgccatatt tcgagcgagt gtaagcgtca gagttggtgt
     2281 tctgcgtatc acccgcatac aggttaccct ggcggctgta gcccgcttcc agttcgagtg
     2341 attgcagagg cgcgaagtcc cagcgcacca cgccgttaat atctttattg atcacccctt
     2401 cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg cgcggactga tggccctggt
     2461 tgatatccca cgcgtcagcc tgggttttgt cgaggttgcc atacaagcgg aagctgaatt
     2521 cgtcgcccag cggaccggtc aggctgaagt tggtgcgttt ggtggcacct tcctctttat
     2581 gttctggcgc attgaaataa gcatcccagg agccgtgcca ctcaccgctg ccttttttgg
     2641 taatgatgtt aaccacgccg cccgccgcgc cgttgccata acgcgcagct gccggaccac
     2701 gtagaacttc aatacgttca atcatttcag gcggcaccca ggaggtatca ccacgggtat
     2761 cgcgctcgcc acgccagccc tgacgcactg agttacggct gcttaccggc ttgccgtcaa
     2821 tcaaaatcag cgtgttttcc ggacccatgc cgcgaatatc aatctggcgg ttattaccac
     2881 gctgaccact ggtggaatta ccggtcaggt taacgccagg catggtacga atgatctccg
     2941 atacatcgcg ggcaaccggg tttttgcgga tttcatctgc ggtaatggtc gaaacgccgg
     3001 gcgcctgcaa gttctgctcg gcggcggtaa caacaatagt atcgtcatgt gaaacaggag
     3061 tatcggtcgg ctcttgtgcc tgtgctaccc cataaatccc cagattgacc aacaaggcca
     3121 gggaatgaat cttcttgttc attgttttat tcctgcattt ttgccacgaa ttgcaactgt
     3181 cgggcatggt cgtcatcaac acgacgcatc ccgctaccgc gaaaaccttt gatcctgaaa
     3241 gacacgcagt gcagctgatt aattaatgtc cgcgcttccc acggcgcgcc attacgctat
     3301 tgcaaatgca aatagttatc aataatatta tcaatatatt tctgcaatca ataaaaaatt
     3361 gcacagtaaa catggggtta tggtgtgacg gcgttaaaag taggaagtga gagctggtgg
     3421 cagtcgaaac atggcccgga atggcaacgt ctgaatgacg aaatgtttga agtcactttt
     3481 tggtggcgtg atccacaagg ttctgaaggc cactcgacga taaagcgcgt atgggtctat
     3541 atcactggca tgaccgatca ccatcagaac agccagcccc ggtcgatgca gcgaattgca
     3601 ggaaccgatg tttggcagtg gacgacacaa ctcaatgcca actggcgcgg cagctactgc
     3661 tttattccta ccgaacgcga tgacattttt tctgcaccat cccccgatcg cctcgaatta
     3721 cgcgaaggct ggcgaaaact attaccccag gcgatagccg atccgctgaa tccacaaagc
     3781 tggaaaggcg ggcgcgggca cgctgtttct gcactcgaaa tgccgcaagc gcctctgcaa
     3841 ccgggatggg attgtccgca agcgccagaa acgcctgcca aagaaattat ctggaaaagt
     3901 gaacggttga aaaattcacg ccgtgtatgg atttttacca ccggcgatgc aacagcagaa
     3961 gaacgcccgc tggcagtttt gcttgatggc gaattttggg cgcagagtat gcccgtctgg
     4021 cccgcgctga cttcgctgac ccatcgtcgg caacttcctc ccgccgtgta tgtgttgatc
     4081 gacgctatcg acaccacgca ccgcgcccac gaactgccgt gtaatgcgga tttctggctg
     4141 gcagtacagc aagagttatt accccaggtg aaaactattg ccccttttag cgatcgcgcc
     4201 gatcgcaccg tggtcgccgg acagagtttt ggtgggcttt ccgcgctgta tgccggactg
     4261 cactggcctg aacgctttgg ctgtgtatta agccagtcag gctcgtactg gtggccgcat
     4321 aggggcgggc accaagaggg cgtgttaatt gaacagctaa aaactggtga agttagcgcc
     4381 gaaggtctgc gcattgtgct ggaagcgggt gttcgcgagc cgatgattat gcaggccaat
     4441 caggcgctgt gtgcgcaatt acacccccta aaagaatcca ttttctggcg tcaggttgac
     4501 ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc aggggctaat cgacctctgg
     4561 caaccacttt tccatgacag gagttgaata tggcattcag taatcccttc gatgatccac
     4621 agggagcgtt ttacattttg cgcaatgcgc aggggcaatt cagtctgtgg ccgcaacaat
     4681 gcgccttacc ggcaggctgg gacgttgtgt gtcagccgca gtcacaggcg tcctgccagc
     4741 agtggctgga agcccactgg cgtactctga caccggcgaa tttcacccag ttgcaggagg
     4801 cacaatgagc cagcatttac ctttggtcgc cgcacagccc ggcatctgga tggcagaaaa
     4861 actgtcggat ttaccctccg cctggagcgt ggcgcattac gttgagttaa ccggagaggt
     4921 tgatgcgcca ctactggccc gcgcggtggt tgccggacta gcgcaagcag atacgttgcg
     4981 gatgcgtttt acggaagata acggcgaagt ctggcagtgg gtcgatgatg cgcagacgtt
     5041 tgaactgcca gaaattatcg acctgcgaac caacattgat ccgcacggta ctgcgcgggc
     5101 attaatgcag gcggatttgc aacaagatct gcgcgtcgat agtggtaaac cactggtctt
     5161 tcatcagctg atacaggtgg cggataaccg ctggtactgg tatcagcgtt atcaccattt
     5221 gctggtcgat ggcttcagtt tcccggccat tacacgccag atcgccaata tttactgcgc
     5281 attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg cctttcgctg atgtagtgga
     5341 agagtatcag cagtaccgcg aaagtgaagc ctggcagcgt gatgcggcat tctgggcgga
     5401 acagcgtcgt caactgccac cgcccgcgtc actttctccg gcacctttac cggggcgcag
     5461 cgcttcggca gatattttgc gcctgaaact ggaatttacc gacggggaat tccgccagct
     5521 ggctacgcaa ctttcaggtg tgcagcgtac cgatttagca cttgcgttgg cagccttgtg
     5581 gctggggcga ttgtgcaacc gcatggacta cgctgccgga tttatcttta tgcgtcgact
     5641 gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac gttttaccgt tgggtattca
     5701 cattgcggcg caagaaacgc tgccgcaact ggcaacccga ctggcagcac aactgaaaaa
     5761 aatgcgccgc catcaacgct acgacgccga acaaattgtc cgtgacagtg ggcgagcggc
     5821 aggagaggaa ccgctgttcg gtccggtact caatatcaag gtatttgatt accaactgga
     5881 tattcctggt gttcaggcgc aaacccatcc cctggcaacc ggtccggtta atgaccttga
     5941 actggccctg ttcccggatg aacacggaga tttgagtatt gaaatcctcg ccaataaaca
     6001 gcgttacgat gagccaacgt taattcaaca cgctgaacgc ctgaaaatgc tgattgccca
     6061 gttcgccgcg gatccggcgc tgttgtgcgg cgatgtcgat attatgctgc caggtgagta
     6121 tgcgcagctg gcgcagatca acgccactca gattgagatt ccagaaacca cgctgagcgc
     6181 gctggtggca gaacaagcgg caaaaacacc ggatgctccg gcgctggcag atgcgcgtta
     6241 ccagttcagc tatcgggaaa tgcgcgagca ggtggtggcg ctggcgaatc tgctgcgtga
     6301 gcgcggcgtt aaaccgggcg acagcgtggc ggtggctcta ccgcgctcgg tctttttgac
     6361 tctcgcgcta catgcaattg ttgaggctgg tgcggcctgg ttaccgctgg ataccggcta
     6421 tccggacgat cgcctgaaaa tgatgctgga agatgcgcgt ccgtcgctgt taatcaccac
     6481 tgacgatcaa ctgccgcgct ttgccgatgt tccagattta acccgccttt gctataacgc
     6541 cccgcttaca ccgcagggca gtgcgccgct gcagctttca caaccgcacc acacggctta
     6601 tatcatcttt acctctggct ccaccggcag gccgaaaggg gtaatggtcg ggcagacggc
     6661 tatcgttaac cgcctgcttt ggatgcaaaa ccactacccg cttacaggcg aagatgtcgt
     6721 tgcccaaaaa acgccgtgca gttttgatgt ctcggtgtgg gagtttttct ggccgtttat
     6781 tgccggggct aaactggtga tggctgaacc ggaagcgcac cgcgacccgc tcgctatgca
     6841 gcaattcttt gccgaatatg gtgtaacgac cacgcacttt gtgccgtcga tgctggcagc
     6901 atttgttgca tcgctgacgc cgcaaaccgc tcgtcagaat tgctcgacgt tgaaacaggt
     6961 tttctgtagt ggtgaagccc taccggctga tttatgccgc gaatggcaac agttaaccgg
     7021 cgcgccgttg cataatctat atggcccgac ggaagcggcg gtagatgtca gttggtatcc
     7081 ggcttttggc gaggaactgg cagaggtgcg cggcagcagt gtgccgattg gttatccggt
     7141 gtggaatacg ggcctgcgca ttctcgatgc gatgatgcat ccggtgccgc cgggtgtggc
     7201 gggcgatctc tatctcaccg gtattcaact ggcgcagggg tatcttggac gacccgatct
     7261 gaccgccagc cgctttattg ccgatccttt tgccccaggt gaacggatgt accgtaccgg
     7321 agacgttgcc cgctggctgg ataacggcgc agtggagtac ctcgggcgca gtgacgatca
     7381 gctaaaaatt cgcgggcagc gtatcgaact gggcgaaatt gatcgcgtga tgcaggcgct
     7441 gccggatgtc gaacaagccg ttacccacgc ctgtgtgatt aaccaggcgg cagccactgg
     7501 tggtgatgcg cgtcagttgg tgggctatct ggtgtcgcaa tcaggtctgc cgttggatac
     7561 cagcgcatta caggcgcagc ttcgagaaac attgccacca catatggtac cggtggttct
     7621 gctgcaactt ccacagttac cacttagcgc caacggcaag ctggatcgca aagccttacc
     7681 gttgcctgaa ctgaaggcac aaacgccggg gcgtgtgccg aaagcgggca gtgaaacgat
     7741 tatcgccgcg gcattctcgt cgttgctggg ttgtgacgtg caggatgccg atgctgattt
     7801 ctttgcgctt ggcggtcatt cgctactggc aatgaaactg gcagcgcagt taagtcgaca
     7861 gtttgctcgt caggtgacgc cggggcaggt gatggtcgcg tcaactgtcg ccaaactggc
     7921 aacgattatt gatggtgaag aagacagcac ccagcgcatg ggattcgaaa ccattctgcc
     7981 gttgcgtgaa ggtaatggcc cgacgctgtt ttgtttccat ccggcatccg gttttgcctg
     8041 gcagttcagc gtgctctcgc gttatctcga tctacaatgg tcgattatcg gcattcagtc
     8101 gccgcgccct catggcccca tgcagacggc ggcaaacctg gatgaagtct gcgaagcgca
     8161 tctggcaacc ttacttgaac aacaaccgca cggcccatat tacctgctgg gttattcact
     8221 tggcggtacg ctggcgcagg gcattgcggc gcgactgcgt gcccgtggcg aacaggtggc
     8281 atttcttggc ttgctggata cttggccgcc agaaacgcaa aactggcagg aaaaagaagc
     8341 caatggtctg gatccggaag tgctggcgga gattaaccgc gagcgcgaag ccttcctggc
     8401 ggcacagcag ggaagtactt caacggagtt gtttaccacc attgaaggca actacgctga
     8461 tgctgtgcgc ctgttgacga cagctcatag cgtagcgttt gatggtaaag cgacgctatt
     8521 tgttgccgaa cgcacgcttc aggaaggtat gagtcctgaa caggcctggg caccgtggat
     8581 tgctgagctg gatatctatc gtcaggattg tgcgcatgtg gatattatct ctccggtggc
     8641 gtttgaaaaa attgggccga ttattcgtgc gacgctaaat aagtaaa
//
LOCUS       DAZGJT010000151         8514 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZGJT010000151 DAZGJT010000000
VERSION     DAZGJT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.154"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(150..956)
                     /gene="trpA"
                     /locus_tag="ACHKFK_004347"
     CDS             complement(150..956)
                     /gene="trpA"
                     /locus_tag="ACHKFK_004347"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO9054553.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAECEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNAAD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVTKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKAFVQPMKAATRS"
     gene            complement(956..2149)
                     /gene="trpB"
                     /locus_tag="ACHKFK_004348"
     CDS             complement(956..2149)
                     /gene="trpB"
                     /locus_tag="ACHKFK_004348"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO9054554.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGHLPDAVIACVGGGSNAIGMFADFINETDVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2161..3519)
                     /gene="trpCF"
                     /locus_tag="ACHKFK_004349"
     CDS             complement(2161..3519)
                     /gene="trpCF"
                     /locus_tag="ACHKFK_004349"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO9054555.1"
                     /translation="MQTVLAKIVADKAIWVEARKQQQPLASFQNEIQPSTRHFYDALQ
                     GARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLPI
                     VSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMGV
                     LTEVSNEEELERAIALGAKVVGINNRDLHDLSIDLNRTRELAPKLGHNVTVISESGIN
                     TYAQVRELSHFANGFLIGSALMAHDDLNAAVRRVLLGENKVCGLTRGQDAKAAYDAGA
                     IYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQLH
                     GNEDQLYIDTLREALPAHVAIWKALSVGETLPARDFQHIDKYVFDNGQGGSGQRFDWS
                     LLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTLR
                     AY"
     gene            complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="ACHKFK_004350"
     CDS             complement(3523..5118)
                     /gene="trpD"
                     /locus_tag="ACHKFK_004350"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO9054556.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="ACHKFK_004351"
     CDS             complement(5118..6680)
                     /gene="trpE"
                     /locus_tag="ACHKFK_004351"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO9054557.1"
                     /translation="MQTQKPTLELLTCKGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALSDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFEN
                     LPQLSAENSCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6702..6795)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="ACHKFK_004352"
     CDS             complement(6772..6816)
                     /gene="trpL"
                     /locus_tag="ACHKFK_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO9054558.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6954..7835
                     /gene="rnm"
                     /locus_tag="ACHKFK_004353"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             6954..7835
                     /gene="rnm"
                     /locus_tag="ACHKFK_004353"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO9054559.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQSIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7832..8452
                     /gene="yciO"
                     /locus_tag="ACHKFK_004354"
     CDS             7832..8452
                     /gene="yciO"
                     /locus_tag="ACHKFK_004354"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO9054560.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
BASE COUNT         2002 a         2261 c         2270 g         1981 t
ORIGIN      
        1 atattactgt tgggcggaaa aaagacgtaa gttgacgttc gacaggggta agcgaaacgg
       61 taaaaagata aataataaat gaatttagga tttttccggc ttcgttaaag aaagttaaaa
      121 tgccgccagc ggaactggcg gctgtgggat taactgcgcg tcgccgcttt catcggttgt
      181 acaaaagctt tcagtgccgc cagcattttc tctggctcat taatatgttg ctcgatgatt
      241 ttaacgatgg ccgaaccaga aatcgcgccc gcagctcctg catcaatcgc tgcttttacc
      301 tgatccgggg cggaaatacc aaatccctgc aatggaggcg cagcgttgta ctctttcagc
      361 ttcgtgacca gatgattgag gggtaacgcg gcgcggtttt ctgcgccggt cacgcccgca
      421 cgtgacaata aataggtgta tccacgaccg taggaggcta tctggcgcag caaatcatcg
      481 gcggcattcg gcgggcaaat aaagataggt gcgacattat gacgcaacgc ggcctggcgg
      541 aagggcgcgg actcttccac gggcacatcg gcaaccagca ccgaatcgac gccgactttc
      601 tcgcactcgg cataaaactc atcaatgcct ttgttaaaca ccaggttggc atacatcaaa
      661 aggccgatgg gaatggtcgg gtgcttctgg cgaatgagtg ccagcatctc aaagcactgc
      721 gccggggtta ctcccgccgc aaaagcacgc agtgtggcgt tttgaatcgt cgggccatcc
      781 gccagtgggt cggagaaggg gatgcctaac tccagcgcgt cagcaccggc ttcaattagc
      841 gtatcgataa ttttcaacga ctgctcaatg cccggatcac cgagggtgac gaaaggaacg
      901 aatgcgcctt ctttgcgctc cttcaactgg gcaaacagag attcgtagcg ttccatcaga
      961 tttcccctcg tgctttcaaa atatcgtgaa cggtgaagat gtctttatcg ccgcgaccgg
     1021 aaaggttaac caccagtagc tgctcttttt ccggattttc gcgcatcatt ttcagcgcat
     1081 gggccagggc gtgggaggat tccagcgccg ggatgatccc ttcatgcagg caaagcgttt
     1141 taaaggcttc cagggcttca tcgtcggtaa tagacacgta atcagcgcgt ccagtgctgt
     1201 tgagatacgc atgttgcggg ccgacggacg ggaaatccag cccggcagaa atggagtaag
     1261 actcttcaat ttgcccgtct tcggtttgca tcatcggcgc tttcataccg aaatagatgc
     1321 ccacgcgacc atgttttaac ggtgcgccgt gctcgccagt ttcgataccg tggccgccag
     1381 gctccacacc aatcaggccg acgtcggttt cgttgatgaa atctgcaaac ataccgatgg
     1441 cattcgaacc accgccaaca caggcgataa cggcatccgg caggtgacct tctctttcca
     1501 gaatctgcgc tttcgtttct tcgccaatca tccgctgaaa ctcacgcaca atggtcgggt
     1561 aaggatgcgg gccagctgcg gtacccagca tatagtgcgc ggtttcataa ctgccggacc
     1621 agtcgcgtag cgcctcatta caggcatctt tcagggtcgc ggaaccgcta tgtaccggga
     1681 tcacttccgc acccattaag cgcatccgga aaacgttagg cgactggcgt tcaacgtctt
     1741 tggcacccat ataaatacgg catttcaggc cgagcagggc gctggcaagg gccgacgcca
     1801 cgccatgctg accggcaccg gtttcggcaa taatttcagt tttacccatc cgcttcgcca
     1861 gtaaagcctg accgagtacc tggttagttt tatgcgcgcc gccgtgcagc aaatcttcgc
     1921 gcttcagata cagcgtggtg ttcgtcccgg ctgtaatgtt ctggcatttg gtcagcgcgg
     1981 ttggacgccc ggcatagttt ttcagcaggt cgttgaactg agcctgaaat tcaggatctt
     2041 tttgcgcgct aacaaaagct tcttccagct ggcgcagagc aggcatcagg atttgtggca
     2101 cgtacatgcc gccaaactca ccaaaatagg ggttaagtaa tgttgtcatt gttcctttcc
     2161 ttaatatgcg cgcagcgtct ggaaaaccga ggccaaaaga cgtgcgtctt tgatacccgg
     2221 ttgcgactct acagcagaat taaaatcaag cccggcgcag ccggtttgtg ccgcttccac
     2281 gcagttatct gcgcctaagc cccccgccag cagaacgttg ccaagcgatt gaccatttaa
     2341 tagtgaccag tcgaaacgtt gtccgctccc gccctgaccg ttgtcgaata catatttatc
     2401 gatgtgctga aaatcgcgcg cgggaagagt ttcaccgaca cttaaagcct tccagatggc
     2461 gacgtgtgct ggcagagcct cacgcagagt gtcgatatac agctgatctt cattaccatg
     2521 cagttgcact gccgccagcg ataacacctt agctttgtcc accacatcgg caatatcgtg
     2581 attgcggaac acgccaacat actgcaacgg tgccgcagcc atcacttcct gcgcctgttc
     2641 aacgttgacg caacgcggtg atgtcgcaac aaaaatcaac ccaccgtaaa tcgcgcccgc
     2701 gtcataagct gctttagcat cttgcccacg tgtcaggcca catactttat tctcacccag
     2761 caacacccga cgcacggcgg cgttcaaatc gtcatgggcc atcaacgccg aaccaatcag
     2821 aaagccgtta gcgaagtggc ttaactcgcg cacctgagcg taagtattga tgccggattc
     2881 gctgattacc gtcacgttgt gccccagttt cggcgcaagc tcgcgggtac ggttgagatc
     2941 aatcgacaaa tcgtgcagat cgcggttgtt gatgccaacg acctttgccc ccaatgcaat
     3001 ggcgcgctcc agttcctctt cattactgac ttcggtcagc acacccatct ccagactgtg
     3061 ggcgacggct gcaagctggc gatattgttc gtcatccagt actgaaagca ttaataagca
     3121 ggcatcggcc tggtaatagc gcgccagata gatctggtaa ggatcgataa tgaagtcttt
     3181 acataaaatc ggctgcgggg cgatttggct gacgatgggg aggaaatcaa agctcccctg
     3241 aaaatatttc tcatcagtca gcactgaaat tgccgaagcg taatgtttat aaatggcagc
     3301 aatgcgtgcc ggatcgaaat catcacggat cacgcctttt gacggcgacg cttttttgca
     3361 ctccagaata aacgccgtgc gtgcaccctg tagcgcatca taaaaatgtc gcgtgctcgg
     3421 ctgaatctca ttctgaaaac tggccagcgg ttgctgctgt ttgcgggctt ctacccaaat
     3481 cgccttgtct gcgacgattt tcgctaaaac ggtttgcatt atttaccctc gtgccgccag
     3541 tgcggtgact ctgtcgtaag cggaaccact gcgcagtacc tcaagaacgg tttgcgcatt
     3601 ggcttgcaga tcttcatggc catgcaggcg cattaacatg gcgacattcg ccgcgacggc
     3661 tgcttcgtga gcggcgtcgc ctttaccttg taacaagcgt gttaaaatgt cacggttttc
     3721 ttccggtgtt ccgcctgcca gttgctcctg gtggtagggt gtcaggccaa aatcttcagc
     3781 ggtcaattga tagctcttaa tttcgccgtc atgcagttcg gcaacgattg tcggcgcgtg
     3841 taatgaaact tcatccatcc cgccgctgtg caccaccgcc gcgcgttgat accccagcac
     3901 gcgcaaggtt tcggcaatcg gcagcaccag ttccggacta taaacaccaa ttaacgccag
     3961 cggcggatgc gccgggttaa tcaatggccc cagcacattg aacagtgtgc gggtttttag
     4021 ttgctggcga accggcattg catggcggaa tccggtgtga tatttcggtg caaagaggaa
     4081 acagacgcct aactcatcca gcgcctggcg cgatttatcg gcgttcatat caagattaat
     4141 accgaacgcc gccagcagat ccgacgagcc ggatttactg gagacgctac ggttgccgtg
     4201 tttcgccact ttcagcccgc aggccgcggc gacaaacgca ctggcggtag aaatattgat
     4261 gctgttactg ccgtcaccgc cggtgccgac gatatcggca aacagataat ccgggcgcgg
     4321 gaacggcgcg gcgttttcca gtagcgcggt tgctgctccg gcgatttcgt tcgggtgctc
     4381 gccgcgaatt ttcatgctca ccagcgccgc cgccagttgt tccggtttca gttcgccacg
     4441 taccaccgct gaaaacagct ggtggctttc ttgttggcta agggtctgcg cctgatacag
     4501 tttttccaga atcggttgca gcgtgttggt tggctcaagt ttctgctgcg cccaggccag
     4561 cgtttgttcc agcaggcgag cgccctgggt agtaagaatg gattccggat ggaactggaa
     4621 tccacaaacg cgatccgcat catgacgcac cgccatcacc atgccattaa aatgggcgtt
     4681 gatggttaaa ccggccggaa tattactgcc aaccagcgag tgataacgcg ccactggcag
     4741 cgggtttgtt aatccggcaa acatcgcctg accgtcatgt tcaatgctcg acgctttacc
     4801 gtgaagaatt tcgcccgcct gaccgacata gcccccgtaa gcttcgacaa tcgcctgatg
     4861 tccgaggcaa atgccaataa ttggcagctt gccacgcaag cgggtgagga gttccggcat
     4921 acaaccggct tcgctcggca caccggggcc aggagaaagc atcagcaccg gattgctcat
     4981 cgtcgccagg cgttcaatta aggtctgcgc cggaatatgg ttgcggtaaa tcaccacgtt
     5041 atgaccattg ctgcgcaact gatctgccag gttgtacgta aaagagtcga tattatcgag
     5101 cagcagaatg tcagccatca gaaggtctcc tgtgcatgat gcgcggtggc aatagcgcgc
     5161 agtacagcgc gggctttatt acgagtttcg tcggcttccg actgcggaac agaatcaagg
     5221 actacgccag caccggcttg cacggtggcg ataccgtttt ccaccagcgc cgagcggatc
     5281 acaatgcagg tgtcgagatc gccatgcgcg gtaaaataac ctaccgcgcc gccgtagctg
     5341 ccgcgtcgac gaccttctgc ttcggcaatt aactgcatag cgcgtacttt cggtgcaccg
     5401 cttaacgtcc ccatattcat acaggcgcgg taagcgtgca gggcgtcgag atcgtggcgc
     5461 agctcaccaa caacgcggga gaccaggtgc atcacgtaag agtaacggtc aactttggtg
     5521 agatcggcga cgtagcggct gccgggtgtg caaatgcgtg ccaggtcatt acgggcgaga
     5581 tccaccagca tcagatgttc agaaagctct ttatgatcgg tacgcatctc cagttcgatg
     5641 cggctgtcga ggtctctgtc cagcgaacca tcggcacgac gaccgcgtgg acgtgttccg
     5701 gcaatcgggt aaatctcaat ctggcggctg gtggcgtcat acttgagcga actttccggc
     5761 gacgcgccaa acagggtgaa atcattatcc tgcataaaaa acatgtacgg gctgggatta
     5821 ctctttttca gcacgtaata ggctgccagc ggtgacgggc agggcagaga gaaacggcga
     5881 gatggcacta cctggaaaat ttctccggcg cgaatcgctt tttgcaacaa acgcactacg
     5941 ccaccgaact cttcatcgct ctggttacat tcacaacgca tatgcggcac ggaaaccacc
     6001 ggcagcggcg gcgcggcttc ggtcagttgc tgacgtagtt cgttaaggcg agcagtgaga
     6061 cgttgttttt cttcttcatt cggagcaaac aggctggcct gaatacgggt gctttttttc
     6121 tgatggtcaa tcaccatcag cgtttcagcg agataaaaac agaaatcagg gcagctattt
     6181 tccgctgaca gttgcggtaa attttcaaat cccgccacaa ggtcataaga gaacaggccg
     6241 ccgaagaaca ttgcttctcg ttcttccttc ggtacattca acagattctg taataagcgg
     6301 aaagcgtcaa aaaccgaaag ggagcataag cgggcgtctt catccagcag tggactgaca
     6361 ggcgggaagc gcagtacgcg gcagtttggt gattgttcat tttccacacc cgcaggcaag
     6421 gcgttatcca gtagtgtcaa cagggcttct ccattgccgg aaagcgcctg gattgtgaca
     6481 gtgtcactta atgctgtaat gcgcagcgca ctgtctacca gcagcaggct ttttaaatca
     6541 tctttgctgt cgatatctgc ggattccagc agcagcgttg ccgggcgatc cccacacaac
     6601 tggtgaaaaa gcgcagtcgg gttgtcgcga taagcgcctt tgcaggttag cagttcgaga
     6661 gtcggttttt gtgtttgcat tgttattctc taattttgtt caaaaaaaag cccgctcatt
     6721 aggcgggctg ggtatctgat tgctttacgc atggtgaata cactgcccgt ttcaggaagt
     6781 gcgccaccaa cctttcagta cgaaaattgc tttcattgtc gatacccttt ttacgtgaac
     6841 ttgtgtacta gttaactagt tcgataatta attgtcaaca gctcatttca gaatatttgc
     6901 cagaaccgtt atgatgtcgg cgcaaaaaac attatccaga acgggagtgc gccttgagcg
     6961 acacgaatta cgcagtgatt tacgacctgc acagccatac cacagcttcc gatggctgcc
     7021 tgacgccaga agcattggtg caccgtgcag tcgagatgcg cgtcggcacc ctggcgatca
     7081 ccgaccatga caccacagct gcaattgcac cagcaagaga ggaaatttca cgttcgggac
     7141 tggcgctgaa tcttattcct ggcgtggaaa tttccacggt ctgggaaaat catgaaattc
     7201 atattgtcgg gctgaatatt gatattactc atccgctaat gtgcgagttc ctcgcgcagc
     7261 agacagaacg gcgcaatcag cgggcgcagc tgattgccga aagacttgaa aaagcgcaaa
     7321 tacctggggc actggaaggc gcgcaacgac tggcgcaggg cggtgcggtg acgcgcggtc
     7381 attttgcgcg tttccttgtg gagtgcggaa aagccagttc aatggcggat gtctttaaaa
     7441 agtatctggc gcgcgggaaa accggatacg ttccgccaca gtggtgtaca atagagcaat
     7501 ctattgatgt cattcatcat tctggcggta aggcggtgct agctcatcct gggcggtaca
     7561 atcttagtgc taaatggctg aaaagattgg ttgcgcattt tgccgaacac cacggcgacg
     7621 cgatggaagt cgcgcagtgc cagcaatcgc ccaatgaacg cacccagctg gcgacacttg
     7681 cgcgtcagca tcatttatgg gcatcacaag gatctgattt tcatcagcca tgcccgtgga
     7741 tcgaactggg tcgtaaactc tggctgcccg caggcgttga aggcgtctgg cagctatggg
     7801 aacagccgca gaacaccaca gagagggaat tatgagccag tttttttata ttcatcctga
     7861 taacccgcag caacgcctga tcaaccaggc ggtggagatc gtgcgtaaag gcggggtgat
     7921 tgtttatcca actgattccg gctatgcgct cggctgtaaa attgaagata aaaacgcgat
     7981 ggagcgtatt tgtcgtattc gccagctgcc ggacggtcac aactttaccc tgatgtgtcg
     8041 cgatctttct gaactctcga cctattcatt tgtcgataac gttgcgtttc gtttgatgaa
     8101 aaacaacacg ccaggcaact ataccttcat cctgaagggg acgaaggagg tgccacgccg
     8161 tctgttgcag gaaaaacgca aaaccatcgg tatgcgtgtg ccgtctaacc cgattgcgca
     8221 ggcgctgctt gaagcattgg gcgaaccgat gctttccact tcgctaatgc tgccaggcag
     8281 tgaatttacc gaatccgatc cggaagaaat taaagaccgt ctggaaaaac aggtggattt
     8341 gattattcac ggcggctatc tcgggcagaa accaacaacg gttattgatc tcaccgatga
     8401 tacgccggtc gtggtgcgtg aaggcgtagg tgatgtgaag cctttcttat aacaaattgc
     8461 gttccacgga tggaagatga tggcagggat atatcgctgc attttgctat tgat
//
LOCUS       DAZGJT010000152         8425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZGJT010000152 DAZGJT010000000
VERSION     DAZGJT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.155"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..1731
                     /gene="iucA"
                     /locus_tag="ACHKFK_004355"
     CDS             7..1731
                     /gene="iucA"
                     /locus_tag="ACHKFK_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO9054561.1"
                     /translation="MILPSEKSATDVAAQCFLNALIRETKDWQLAEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RQEAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDL
                     NGNIMQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLM
                     ARVRTLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            1732..2679
                     /gene="iucB"
                     /locus_tag="ACHKFK_004356"
     CDS             1732..2679
                     /gene="iucB"
                     /locus_tag="ACHKFK_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO9054562.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2679..4421
                     /gene="iucC"
                     /locus_tag="ACHKFK_004357"
     CDS             2679..4421
                     /gene="iucC"
                     /locus_tag="ACHKFK_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO9054563.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARHGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4418..5751
                     /gene="iucD"
                     /locus_tag="ACHKFK_004358"
                     /pseudo
     CDS             4418..5751
                     /gene="iucD"
                     /locus_tag="ACHKFK_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016528830.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
     gene            5777..7978
                     /gene="iutA"
                     /locus_tag="ACHKFK_004359"
     CDS             5777..7978
                     /gene="iutA"
                     /locus_tag="ACHKFK_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO9054564.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAVFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1992 a         2192 c         2227 g         2014 t
ORIGIN      
        1 ttgattatga tcctgccctc tgaaaaatcc gccacagatg tggctgcgca gtgcttcctg
       61 aatgccttga ttcgtgaaac caaagactgg caactggcgg aatacccgcc agacgaattg
      121 attatcccgc tggacgaaca gaagtcgctc catttcagag tggcttattt ctccccaacc
      181 cagcatcacc gctttgcatt tcctgctcat ctggtcacgg catcaggcag ttatcctgtc
      241 gactttacca ctctctcccg gcttattatt gataagctcc ggcatcaact ttttctgcca
      301 gttcccctct gcgaaacttt ccaccaacgc gtgctggaaa gctacgccca tacgcaacag
      361 acaattgatg cccgccatga ctgggccatc ctgcgtgaaa aagcgttgaa ttttggcgag
      421 gctgagcagg cactgctgac aggacacgct ttccaccctg cacctaagtc tcatgaaccg
      481 tttaaccgcc aggaagctga acgatacctg cctgacatgg cacctcactt cccgctgcgg
      541 tggttttcgg tggataaaac gcaaatcgct ggtgaaagtc tgcatcttaa ccttcaacag
      601 cggttgacgc gatttgccgc agaaaatgcg ccccagttac tcaacgaatt aagtgacaat
      661 caatggctgt tcccgctgca cccgtggcag ggagaatatc ttttgcagca agtgtggtgc
      721 caggcacttt ttgctaaagg acttatcaga gacttaggcg aggccggcac gtcgtggctg
      781 ccgaccacct cttcccgctc cctctactgt gctaccagcc gcgatatgat caagttctcc
      841 ctgagcgttc ggctgaccaa ctccgtccgt actctgtctg tgaaagaggt ggagcgagga
      901 atgcgcctgg cacgtctggc gcaaaccgac ggctggcaga tgctacaggc ccgcttccct
      961 actttccggg taatgcagga ggacggctgg gccgggctgc gcgatcttaa cggcaacatc
     1021 atgcaggaaa gtttgttttc cctgcgtgaa aacctgctgc tggagcagcc gcaaagccag
     1081 accaacgttc tggtttccct gactcaggcc gcaccggatg gcggtgattc gctgctggtt
     1141 tcggcggtaa aacgcctgag cgatcgcctc ggtatcactg tgcaacaggc cgcccacgca
     1201 tgggtcgatg cttactgtca gcaggtgctg aagcctctgt ttacggctga agcggattac
     1261 ggcctggtgc tgctggcgca tcagcaaaat attcttgtgc agatgcttgg ggatctgccg
     1321 gtcggattta tttaccgtga ctgccagggc agcgcgttta tgcctcacgc gacagagtgg
     1381 ctcgatacca ttgacgaggc gcaggcggaa aatattttca cccgtgagca gttgctgcgc
     1441 tatttccctt attacctgct ggttaactcc acttttgccg taactgctgc gctgggtgct
     1501 gccgggctgg acagcgaagc gaatctgatg gctcgtgtac gaacattgct ggctgaagtg
     1561 cgtgaccagg tgactcataa aacctgtctc aactatgttc tggaaagtcc gtactggaac
     1621 gtaaaaggta actttttctg ttatctgaac gatcataacg agaacaccat cgttgaccct
     1681 tcggtgatct attttgattt cgctaacccg ctgcaggctc aggaggtctg aatgtctggg
     1741 gcaaacattg ttcacagcgg atatggactg cgctgtgaaa aactcgacaa gcctctgaat
     1801 cttggctggg ggctggacaa tagcgcggtg ttgcactggc ccggggagct gccaacaggg
     1861 tggctgtgcg acgcgctgga tcagatattt atcgccgcac cacaactttc agcagtggtt
     1921 cttccctggt ccgaatggtg tgaggagcca caggcgctga cgcttttcgg acaggtacaa
     1981 agcgacatta tccatcgttc cgctttctgg cagttaccgt tatggctgag ttctccggca
     2041 aaccgggcct ccggtgaaat ggtttttgat gcagagcgtg agatttattt cccgcagcgc
     2101 cccccccgtc cgcagggtga agtttatcgt cgttacgatc cacgcattcg caggatgctg
     2161 agtttccgca ttgccgatcc cgtttctgat gcagaacgtt tcactcgctg gatgaacgat
     2221 ccgcgcgttg agtatttctg ggagcaaagt ggctcactgg aggtacagat cgcctatctg
     2281 gaacgccagc tcaccagtaa acatgcattc ccgctgattg gttgtttcga cgatcggccg
     2341 ttcagttatt tcgaaatcta ctgggcggcg gaagaccgca ttggtcgcca ctattcgtgg
     2401 cagccctttg accgtggcct gcatctgctg gttggtgaac agcaatggcg cggagcccac
     2461 tatgtgcaaa gctggctgcg cggggtgaca cattacctgc tgctgaatga gccacgtacg
     2521 cagcgcacag tactggagcc acgcaccgat aaccagcgcc tgttccgcca tcttgagcct
     2581 gcgggatacc ggacaattaa agagttcgac ttcccacata aacgctcgcg catggtgatg
     2641 gcggatcgtc atcacttctt cacggaggtc ggtctgtaat gaatcacaaa gactgggatt
     2701 tggtcaaccg ccgcttggtg gcaaaaatgt tgtctgagct ggagtatgag caggttttcc
     2761 acgcggaatc tcagggcgat gaccgctact gcattaacct gccgggagca caatggcgct
     2821 tcatcgctga acgtggtatc tggggctggc tctggattga tgctcaaact ctgcgctgcg
     2881 cggacgagcc agtactggct cagacgctgc tgatgcagct aaagcaggta ctgtcaatga
     2941 gcgatgcaac cgttgctgag catatgcagg atttgtatgc cacgctgctg ggcgacctgc
     3001 aactactgaa agcccgtcac gggctgagcg ccagtgacct gattaatctt aatgccgacc
     3061 gcctgcaatg cctgctgagc ggtcatccta aattcgtttt taataaaggt cgccgtggct
     3121 ggggtaaaga ggcgctggaa cgatatgcgc cagagtatgc caacaccttc agactgcact
     3181 ggctggcggt aaaacgtgaa catatgatct ggcgctgtga taacgagatg gatattcatc
     3241 agttgttgac ggccgcaatg gatccgcagg agtttgcccg cttcagtcag gtctggcagg
     3301 aaaacggact ggatcataac tggctgccgc tgccggtaca tccgtggcag tggcagcaaa
     3361 aaatcgctac cgacttcatc gctgattttg ccgaaggcag gatggtgtct ctcggcgagt
     3421 ttggcgacca gtggctcgcc cagcagtcgc tgcgtaccct gaccaacgcc agtcgccggg
     3481 gcgggctgga tatcaagctg ccgctgacca tctacaacac ctcatgctac cgggggatcc
     3541 ctggcagata catcgctgcc ggaccactgg cttcacgctg gctacaacag gtttttgcga
     3601 ccgacgccac cctagtgcaa agcggcgcag tgatacttgg tgaaccggct gcaggctatg
     3661 tctcccatga aggctatgcc gcgcttgccc gggctcccta tcgctaccag gaaatgcttg
     3721 gtgttatctg gcgggagaat ccgtgccgct ggctgaaacc ggatgaaagt ccggttctga
     3781 tggcaacact gatggagtgc gacgaaaaca atcagccgct ggcaggcgca tatatagacc
     3841 gctccgggct ggacgctgaa acctggctta cgcaactgtt ccgggtggtc gtggttcctc
     3901 tgtatcacct gctttgccgc tacggtgtcg cgcttattgc acatggacaa aatataactc
     3961 tcgccatgaa agagggggtt ccacagcgtg ttctgctgaa agacttccag ggcgatatgc
     4021 gactggtgaa agaagagttc cccgaaatgg actctttgcc tcaggaggtt cgtgatgtta
     4081 catcccgcct gagtgcggac tacttaatcc atgatttgca gacgggtcac ttcgtgacag
     4141 tactgcgttt tatttcgcca ctgatggttc gtcttggcgt acctgaaagg cgattttatc
     4201 aactgctggc agcagtgttg agtgattaca tgaaaaaaca tccacaaatg tcagagcgtt
     4261 ttgcgctttt ctcacttttc aggccacaaa tcattcgcgt ggtgctgaac ccggtaaaac
     4321 tgacctggcc tgatctggat ggcggcagcc gcatgctgcc gaattacctt gaggatctgc
     4381 aaaatccgct gtggctggta actcaggaat atgaatcatg aaaaaaagtg tcgattttat
     4441 tggtgtaggg acagggccat ttaatctcag cattgctgcg ttgtcacatc agatcgaaga
     4501 actggactgt ctcttctttg atgaacatcc tcatttttcc tggcatccgg gtatgctggt
     4561 accggattgt catatgcaga ccgtctttct gaaagatctg gtcagtgctg ttgcacctac
     4621 aaatccctac agttttgtta actatctggt gaagcacaaa aagttctatc gcttccttac
     4681 aagcagacta cgtacagtat cccgtgaaga gttttctgac tacctccgct gggctgctga
     4741 agatatgaat aacctgtatt tcagtcatac cgttgaaaac attgatttcg ataaaaaacg
     4801 tcgattgttt ctggtgcaaa ccagccaggg agaatatttt gcccgcaata tctgccttgg
     4861 tacaggaaaa caaccttatt taccaccctg tgtgaagcat atgacacaat cctgtttcca
     4921 tgccagtgaa atgaatcttc gtcggccgga ccttagtgga aaacggataa ccgtggttgg
     4981 tggaggacag agtggtgcag acctgttcct taatgcatta cgcggggaat ggggagaagc
     5041 ggcggaaata aactgggtct cccggcgtaa taattttaac gcactggatg aggctgcttt
     5101 tgctgatgag tattttacac ctgaatatat ttcaggcttc tccggactgg aggaagatat
     5161 tcgccatcag ttactggatg agcagaaaat gacatcggat ggcatcactg ccgattcttt
     5221 actgaccatt tatcgtgagt tgtaccaccg ttttgaagtt ctgagaaaac caagaaatat
     5281 ccgtctgcta cccagccgct cggtaacaac tctggaaagt agtggtcctg gctggaagtt
     5341 gctgatggag catcatctgg atcagggcag ggagagcctg gaaagtgatg tggtgatttt
     5401 cgccacaggt taccgttctg cgttgccaca aatacttccc tcactgatgc ccctgatcac
     5461 catgcacgat aagaacacct ttaaagtgcg tgatgacttc actctggaat ggagtggccc
     5521 gaaagagaac aacatcttcg tggtcaacgc cagtatgcaa acccatggca tcgccgaacc
     5581 ccagctcagc ctgatggcat ggagatctgc acgtattctt aatcgcgtaa tgggacgtga
     5641 tttattcgat ctcagtatgc cgcccgccct gattcagtgg cgcagcggca cctagggaaa
     5701 acgcagccgg aggctgcttc tttaactcgc tacacagcat ctttgggctg attttttccg
     5761 cccgtatgga ggaataatga tgataagcaa aaagtatacg ctttgggctc tcaacccact
     5821 gcttcttacc atgatggcgc cagcagtcgc tcaacaaacc gatgatgaaa cgttcgtggt
     5881 gtctgccaac cgcagcaatc gcaccgtagc ggagatggcg caaaccacct gggttatcga
     5941 aaacgccgaa ctggaacagc agattcaggg cggcaaagag cttaaagacg cactggctca
     6001 gctgatccct ggccttgacg tcagcagccg gagccgcacc aactacggta tgaatgtgcg
     6061 tggccgcccg ctggtcgtgc tggttgacgg cgtgcgtctc aactcttcac gtaccgacag
     6121 ccgacaactg gactctatag atccttttaa tatcgaccat attgaagtga tctccggtgc
     6181 gacgtccctg tacggcggcg gcagtaccgg tggcctgatc aacatcgtga ccaaaaaagg
     6241 ccagccggaa accatgatgg agtttgaggc tggcaccaaa agtggcttta gcagcagtaa
     6301 agatcacgat gaacgcattg ccggagctgt ctccggcgga aatgagcata tctccggacg
     6361 tctttccgtg gcatatcaga aatttggcgg ctggtttgac ggtaacggcg atgccacctt
     6421 gcttgataac acccagaccg gcctgcagta ctccgatcgg ctggacatca tgggaactgg
     6481 tacgctgaac atcgatgaat cccggcagct tcagttgatc acacagtact ataaaagcca
     6541 gggcgacgac gattacgggc ttaatctcgg gaaaggcttc tctgccatca gagggaccag
     6601 cacgccattc gtcagtaacg ggctgaattc cgaccgtatt cccggcactg agcggcattt
     6661 gatcagcctg cagtactctg acagcgcttt tctgggacag gagctggtcg gtcaggttta
     6721 ctaccgcgat gagtcgttgc gattctaccc gttcccgacg gtaaatgcga acaaacaggt
     6781 gacggctttc tcttcgtcac agcaggacac cgaccagtac ggcatgaaac tgactctgaa
     6841 cagcaaaccg atggacggct ggcaaatcac ctgggggctg gatgctgatc atgagcgctt
     6901 tacctccaac cagatgttct tcgacctggc tcaggcaagc gcttccggag ggctgaacaa
     6961 caagaagatt tacaccaccg ggcgctatcc gtcgtatgac atcaccaacc tggcggtctt
     7021 cctgcaatca ggctatgaca tcaataatct ctttaccctc aacggtggcg tacgctatca
     7081 gtacactgaa aacaagattg atgatttcat cggctacgcg cagcaacggc agattgccgc
     7141 cgggaaggct acatccgccg acgccattcc tggcggctca gtcgattacg acaacttcct
     7201 gttcaacgcc ggtctgctga tgcacatcac cgaacgccag caggcatggc tcaacttctc
     7261 ccagggcgtg gagctgccgg acccgggtaa atactatggt cgcggcatct atggtgctgc
     7321 agtgaacggc catcttcctc taacaaagag tgtgaacgtc agcgacagca agctggaagg
     7381 cgtgaaagtc gattcttatg agctgggctg gcgctttact ggcaataatc tgcgtaccca
     7441 aatcgcggcc tactattcga tttctgataa gagcgtggtg gcgaataaag atctgaccat
     7501 cagcgtggtg gacgacaaac gccgtattta cggcgtggaa ggtgcggtgg actacctgat
     7561 tcctgatact gactggagta ccggagtgaa cttcaacgtg ctgaaaactg agtctaaagt
     7621 gaacggtacc tggcagaaat acgatgtgaa gacagcaagc ccatcaaaag cgacagccta
     7681 cattggctgg gcaccggacc cgtggagtct gcgcgtgcag agcaccacct cctttgacgt
     7741 gagcgacgcg cagggctaca aggtcgatgg ctataccacc gtggatctgc tcggcagtta
     7801 tcagcttccg gtgggtacac tcagcttcag cattgaaaac ctcttcgacc gtgactacac
     7861 cactgtctgg gggcagcgtg caccactgta ctacagcccg ggttacggcc cagcgtcact
     7921 gtacgactac aaaggcaggg gccgaacctt tggtctgaac tactctgtgc tgttctgacc
     7981 ggtattcctt tacaacaaag gtacgctgat atcaacatgg ccgctgacag ccaagttgat
     8041 atcatataat acacgacata atctgtagtc agggaggata gactctttac tgactacaga
     8101 ttatgtcgtg ttccgtgctc atttcctcaa aaaaatacaa gaaaagaatt agtattctaa
     8161 caaaaagtga aataaattgt atcaaactcc ctcttttaat cctgttgagt aaatcagctt
     8221 ttgcaatagg attgaaagag tgtaagtgga atctcttccg gatactcgtt accgccgtgg
     8281 ctagaatatc tacggctgcg ggccagtaat agattaagtg ataacagctg tctggaaatg
     8341 taggggcgta tccactaatc ataatcattg tgagataaat attacggggc attttataat
     8401 gaataaaata tactctctaa aatat
//
LOCUS       DAZGJT010000153         8344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZGJT010000153 DAZGJT010000000
VERSION     DAZGJT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.156"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..845
                     /locus_tag="ACHKFK_004360"
     CDS             174..845
                     /locus_tag="ACHKFK_004360"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO9054565.1"
                     /translation="MDKVLDSAILSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIATVVMTTGNSGVDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     ENIIKNQIPSDVEIIRVYTMHDADRHQDHLAVYQASMVACRAIPQILGYETPSTWLSF
                     MPQVFESVKEEYFSLKLTALKKHKSQSQRDYMRPERLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            855..2051
                     /gene="yaiP"
                     /locus_tag="ACHKFK_004361"
     CDS             855..2051
                     /gene="yaiP"
                     /locus_tag="ACHKFK_004361"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO9054566.1"
                     /translation="MKTWIFICMAVAILLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVLYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWVGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSHPSVTGT
                     EKLSEA"
     gene            2086..2610
                     /locus_tag="ACHKFK_004362"
     CDS             2086..2610
                     /locus_tag="ACHKFK_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO9054567.1"
                     /translation="MIIDESAGEVVIGANTRICHGAVIQGPVVIGTNCLIGNYAFIRP
                     GTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVANQAYLGAQVRTSNHRLDEQ
                     PVSVRTPDGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQLGPRVIVERNLPT
                     GTYSLRQELIRTGD"
     gene            2612..3385
                     /gene="yaiO"
                     /locus_tag="ACHKFK_004363"
     CDS             2612..3385
                     /gene="yaiO"
                     /locus_tag="ACHKFK_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO9054568.1"
                     /translation="MIKRTLLAAAIFSALPVYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGISLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSISLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3574..3849
                     /gene="frmR"
                     /locus_tag="ACHKFK_004364"
     CDS             3574..3849
                     /gene="frmR"
                     /locus_tag="ACHKFK_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO9054569.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3884..4993
                     /gene="frmA"
                     /locus_tag="ACHKFK_004365"
     CDS             3884..4993
                     /gene="frmA"
                     /locus_tag="ACHKFK_004365"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO9054570.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTPRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFELARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5086..5919
                     /gene="fghA"
                     /locus_tag="ACHKFK_004366"
     CDS             5086..5919
                     /gene="fghA"
                     /locus_tag="ACHKFK_004366"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO9054571.1"
                     /translation="MELIEKHASFGGWQNVYRHYSPSLKCEMNVGVYLPPKAENEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPNLVMHHFPATARKSISGHSMGGLGALVLALRNPD
                     EYASVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6146..6685)
                     /gene="yaiL"
                     /locus_tag="ACHKFK_004367"
     CDS             complement(6146..6685)
                     /gene="yaiL"
                     /locus_tag="ACHKFK_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO9054572.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKITQTQLINGRLAIARLLVDNNNEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6787..7998)
                     /gene="mhpT"
                     /locus_tag="ACHKFK_004368"
     CDS             complement(6787..7998)
                     /gene="mhpT"
                     /locus_tag="ACHKFK_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO9054573.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILISSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8174..>8344)
                     /locus_tag="ACHKFK_004369"
     CDS             complement(8174..>8344)
                     /locus_tag="ACHKFK_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308434.1"
                     /note="catalyzes the formation of pyruvate and
                     acetaldehyde from 4-hydroxy-2-ketovaleric acid; involved
                     in the degradation of phenylpropionate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO9054574.1"
                     /translation="LGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDM
                     IVDVALDLRNNK"
BASE COUNT         2062 a         2146 c         2068 g         2068 t
ORIGIN      
        1 atatgaacta cataaaagat tacacactct tatccccgta cctgacagcc ccttgttttt
       61 cagctatcag ctatttttaa cacttaaaaa ttagcatctg tctgcgtcag aggttacgtc
      121 tctgggggcg tcgttgtgct tgtagtataa taaataaaat ggagtgtcat aaagtggata
      181 aggttttaga ttcagcgatc ctttcttcgg caaataaaag aaagggtatc cttgccattg
      241 gcgcgcatcc tgacgatata gaattaggct gtggcgcatc gctagctcgc cttgcgcaaa
      301 aaggaattta tattgctacc gtggtgatga ctaccggtaa ttctggcgta gatggaataa
      361 tcgatcgcca cgaagaatca cgcaacgctc taaagatatt agggtgccac caaactattc
      421 atcttaattt tgctgacacc cgcgctcatt tacagctcaa tgatatgatt tctgccctgg
      481 aaaacatcat aaaaaatcaa attccctctg atgttgaaat tatacgtgta tataccatgc
      541 atgatgctga ccgccatcag gatcatcttg cagtctatca agcctcaatg gttgcctgtc
      601 gcgctattcc gcaaattctc ggctatgaaa caccaagtac ctggctttca tttatgcctc
      661 aggtttttga atccgtgaaa gaagaatatt tttctcttaa actgactgcg ttaaaaaaac
      721 ataaaagtca gagccagcga gattatatgc gcccggagcg tctgcgtgca gttgctcaat
      781 ttcgcgggca acaggtcaat agcgatctgg gtgaaggttt tgttattcat aaaatgattc
      841 tttgagcaaa ttgcatgaaa acctggatct ttatatgtat ggccgtagca atattgctat
      901 ggtttctgag tacgttaagg cgtaagccca gccaaaagaa aggctgtatt gacgccatta
      961 tacctgctta taacgaaggc ccgtgtctgg cgcagtcact ggataatctg ctgcgtaacc
     1021 cttatttttg ccgggtaatt tgcgttaacg acggctccac ggacaatacc gaagcggtca
     1081 tggcggaagt caaacgcaaa tggggcgacc gctttgttgc cgtcacgcaa aaaaataccg
     1141 gtaaaggtgg tgcgctgatg aatggcctca attacgccac ctgcgatcag gtttttttaa
     1201 gtgatgccga cacctatgtt ccgcccgatc aagacggaat gggctatatg ctggcggaaa
     1261 ttgagcgtgg cgctgatgcc gtaggcggca ttccctctac tgcgttgaaa ggcgcgggtc
     1321 tgttaccaca catccgcgcg accgtaaagt tgccgatgat tgttatgaag cgcacgctac
     1381 agcagcttct gggcggcgca ccgtttatca tcagcggtgc ctgcgggatg ttccgtactg
     1441 atgtattgcg taagttcggt ttctcggatc gtactaaagt cgaagacctt gatctcacct
     1501 ggacattggt ggcaaacggc taccgtattc ggcaggcgaa tcgctgcatc gtatacccac
     1561 aggaatgtaa cagtccgcgt gaggagtggc gccgctggcg gcgttggatt gtgggctacg
     1621 cggtctgtat gcgcctgcat aaaagacttt tatttagccg cttcggtatc ttcagtatat
     1681 ttcctatgct gttggttgtg ctttatggcg ttggtattta tctcactacc tggtttaatg
     1741 aattcatcac caccgggcca catggtgtgg tgttggcaat gttcccgctt atctgggtcg
     1801 gcgtagtttg tgttattggt gcatttagcg cctggtttca tcgttgctgg ttgttggtgc
     1861 ctttagcacc gctttccgtt gtgtatgtat tattagctta tgccatctgg attatttatg
     1921 gacttattgc cttttttact ggacgcgaac ctcagcgcga caaacccacc cgctattccg
     1981 cactggtgga agcgtcaacc gcttattccc acccttctgt cacaggaact gaaaaactat
     2041 ctgaagctta atgttaaaga gaaaaatatt cagattgcag accaggtgat tattgatgaa
     2101 agtgccggtg aggttgttat cggcgcgaat acccgtattt gccacggtgc cgttattcag
     2161 ggtccggtag tgattggcac aaactgcctg ataggtaatt atgcgtttat tcgtcctggc
     2221 acaataatca gcaatggcgt aaaaattggt tttgccaccg aaattaaaaa tgcggttatt
     2281 gaagcggaag caacgattgg tccgcaatgc tttattgccg actcggtagt tgcaaaccag
     2341 gcatatttgg gcgcacaagt acgaaccagt aatcatcgtc tggatgaaca gcccgtgtct
     2401 gttcgaactc cagatggaat tatcgctacc ggatgcgata aattaggttg ttatatcggg
     2461 cagcgttcac gccttggcgt acaagttatt attttgcctg ggcgaattat ttccccgaac
     2521 acgcagcttg gcccgcgcgt gattgtagaa cgtaatttac ctaccggaac ttattcactc
     2581 cgacaagaac ttatccgtac aggagattaa aatgataaaa cggacgttat tagcggcggc
     2641 aatttttagc gcattacccg tttatgccgg gttaacttcc attaccgcgg gctacgattt
     2701 taccgattat tctggcgatc atggcaaccg taatttagcg tatgctgaac tggtggcgaa
     2761 agttgaaaac gcaacgctgc tttttaatct ttcccagggg cgtcgtgatt atgaaactga
     2821 acatttcaat gccactcgcg gccagggtgc agtctggtac aaatggaata actggctgac
     2881 aacccgaacg ggtattgcct ttgcggataa tacgccggtc tttgcccgcc aggattttcg
     2941 tcaggatatt aacctggccc tgttgccaaa aacgcttttc acgaccggtt atcgctacac
     3001 taaatattac gatgatgtcg aagtcgatgc ctggcaaggt ggcatatcac tctatactgg
     3061 cccggtgatc accagctatc gctataccca ttatgactcc agcgatgcag gtggtagtta
     3121 cagcaatatg atttccgtgc gtctgaatga cccgcgcggc actggttaca cgcaactatg
     3181 gctaagccgc ggaacaggtg cttacaccta cgactggacg ccagaaacac gctacggcag
     3241 catgaagagc atcagtttgc aacgtattca accgctgact gagcaactta atctcgggtt
     3301 gacggcaggt aaagtgtggt acgacacccc aaccgatgat tataacggtc tgcaacttgc
     3361 agcccatctg acctggaaat tctgattcct tctgccgccc gctatccggg gcggccttcc
     3421 ctgccgatta gcccctatcc cctttcctct ttgttttccg actacattca ccggataaat
     3481 tttattctcc agtgttatat actatagggg ggtatgcatt gacatatagc atacccccct
     3541 atagtatatt gcgtgcagat gatgaggtgc gaaatgccca gtactccgga agagaagaaa
     3601 aaggtcctta ctcgagttcg tcgtattcgg gggcagattg atgctctgga acggtcgctg
     3661 gagggtgatg ccgaatgccg tgccatactc caacagatcg ctgccgttcg gggcgcggct
     3721 aacggactga tggcagaagt gcttgaaagc catatccggg aaacgtttga ccgaaatgac
     3781 tgctacagcc gcgaagtcag ccaatccgtt gacgacacta ttgaactggt tcgtgcctat
     3841 cttaaatagc tgaatctatt accatattga ggaagagcga gagatgaaat cacgtgctgc
     3901 cgttgcattt gctcccggta aaccgctgga aatcgttgaa attgacgttg caccaccgaa
     3961 aaaaggtgaa gtgctgatta aagtcaccca taccggtgtt tgccataccg acgcatttac
     4021 cctctccggc gatgacccgg aaggtgtctt cccggtggtt ctcggtcacg aaggggccgg
     4081 ggttgttgtt gaagtcggtg aaggcgtaac cagcgtcaaa cctggcgacc atgtgatccc
     4141 gctttacacc gcagagtgcg gcgagtgtga gttctgtcgt tctggcaaaa ccaacctgtg
     4201 tgtcgcggtt cgcgaaacgc agggcaaagg cttgatgcca gacggcaccc cccgtttttc
     4261 ttacaacggg cagccgcttt atcactacat gggatgctca acattcagtg aatacaccgt
     4321 ggtagcggaa gtgtctctgg ccaaaattaa tccagaagca aaccatgaac acgtctgcct
     4381 gctgggctgt ggcgtgacca ccggtattgg cgcggtacac aacacagcta aagtccagcc
     4441 aggtgattct gttgccgtgt ttggtcttgg cgcgattggt ctggcagtgg ttcagggcgc
     4501 gcgtcaggcg aaagcgggac ggattatcgc tatcgatacc aacccgaaga aattcgaact
     4561 ggcgcgtcgc ttcggtgcca ccgactgcat taacccgaat gactacgaca aaccaattaa
     4621 agatgttctg ctggatatca acaaatgggg tatcgaccat acctttgaat gcatcggtaa
     4681 tgtcaacgtg atgcgtgcag cgctggaaag cgcgcaccgc ggctggggtc agtcggtgat
     4741 catcggggta gcaggctccg gtcaggaaat ctccacccgt ccattccagt tggtcaccgg
     4801 tcgcgtatgg aaaggttccg cgtttggcgg cgtgaaaggt cgttcccagt tacctggtat
     4861 ggttgaagat gcgatgaaag gtgatatcga tctggaaccg tttgtcacgc ataccatgag
     4921 cctggatgaa attaatgacg ccttcgacct gatgcatgaa ggtaaatcca ttcgaaccgt
     4981 aattcgttac tgatttcccg ctggtttacc ccgtccagtt cagacggggt tcttaatact
     5041 ctccccgggc agccgtccgg gggattaacc gtgagataat gactgatgga actcattgaa
     5101 aaacatgcca gctttggcgg ctggcaaaat gtgtatcggc attattcccc atcactgaaa
     5161 tgtgaaatga atgtcggcgt ctatctccca ccaaaagccg agaatgaaaa attgccggtg
     5221 ttgtactggc tttcaggcct gacctgcaac gagcagaatt tcattactaa atcggggatg
     5281 cagcgttacg cggctgagca caacattatt gttgttgcgc cggacaccag tccgcgaggc
     5341 agtcatgtcg cagatgctga ccgttacgat ctcgggcaag gtgccgggtt ttacctgaac
     5401 gcgacgcaag cgccgtggaa tgaacattac aaaatgtatg actatatccg caacgagctg
     5461 ccgaatttag tgatgcatca ttttccagca acggccagaa agtctatctc aggtcattct
     5521 atgggcgggc tgggcgcgct ggtgctggcg ttacgtaacc cagatgaata tgccagcgtc
     5581 tcggcgtttt cgcccattgt ctccccatcg caagtaccgt ggggacagca agcctttgcc
     5641 gcatatcttg gtgaaaataa agatgcctgg ttggattacg acccggtgag tcttatttca
     5701 caaggtcaac gcgttgcgga aatcatggtt gatcagggat tgagtgatga tttttacgca
     5761 gaacagctgc ggactccaaa tcttgaaaag atctgccagg agatgaatat caagacgtta
     5821 atccgttatc acgagggtta tgatcacagc tattattttg tctccagttt tattggcgag
     5881 catattgcgt accacgccaa taaactgaat atgcgttgat aatagtgcac gactgccgga
     5941 tgcggcgtaa acgccttatc cggcccacat ttcgctcgta aaccgtaggc ctgataagac
     6001 gcgaaaagcg tcgcatcagg catctgcgca cggctgccgg atgcggcgtg aacgccttat
     6061 ccggcctaca cttcgcccat aaaccgtagg cctgataaga ctcgcaaagc gtcgcatcag
     6121 gcatctgcac acgatttatc tatgtttacc acatcaaatc atcaggcact ttgaagtcgg
     6181 catacggatc gtcttcatcc tgctcttcgg cgctgagcgc gctgtgcaac acaatactgc
     6241 tggcatcacg ctgagcaatt ttatcggcaa cgctggcggg aataatggcg tattcacctt
     6301 cgttattgtt atcgaccaac aagcgggcaa tcgccagacg accattaatc aactgcgttt
     6361 gcgtgatttt atcgacaaag attttcttaa tcagattgcc gtcagtgaag ttaaaaccaa
     6421 tatcgccatt ggcaatggta attcggttca tttcaatgag ctgcttcact tgggctttat
     6481 attctttcgc caacgccgct tgtttttgtt gctcgctaag ctgtttatca cgctcaagct
     6541 gtgccttttt attttcttct accgccgcac gagcttcacg cgcctgaaca cgcgattttt
     6601 tcgccgttct ctccaccttc gccgcttttt tgctggtgac tagtcccgct ttgagcaact
     6661 gctcttgtaa ggtaagtttt gccatctttg tttccaaacc cgctgaataa tttttagcga
     6721 gtatacctgt aaacgccgta ggtgtattgg gggcaagtcc ctttcgcccc tacttaataa
     6781 ggcgcgtcag gcatcggcgc acggctgcat tcgtgatctc cggctcatca aaataaacac
     6841 tgccaggccg gcaacaagaa tacccggcgc agaagcggcc attacgccaa ccgtgccagt
     6901 gcctaatgcc agcattttcc cggccagtaa cggaccgctc atagccccca gacgccctac
     6961 cgccacggct gttcccacac ctgttgcgcg gatctgcgaa ctgtaaaaca acggtgccag
     7021 ggcatacaaa acgctttgcc cacctgtcgc aaacaacccc gcgacgaatc ccgccagcaa
     7081 cataccgtta aatgacgaca ccgttcccag cgccagcagc gaagctaaca tgccgctata
     7141 aatcagtagc gacatggtta ctggacgcag cttatccatc aatgcgccca acattaacgt
     7201 cccgcttgcc gcacccattt gtagggcaaa catcacccct gccgcctgcg atggctggaa
     7261 tccttgctcc accaaaagca gcggtagcca gttgatcaac atgtagacca ccagcagagt
     7321 gaagaaataa cacaaccaca gcagcagcgt cgcggtagcc gtttctggcg caaataacgc
     7381 acgcagtggt ggcgcagcct gtttttcgcc agcaaaaacc gccgactccg gcagccagcg
     7441 cattaatagc ggcaccagaa tcaacggcac cacaccacct acccaaaaca ccgtttgcca
     7501 tgctaagttt gcccccgcga aacccagcgt cgccgccagc gccgcgccaa tgggaacacc
     7561 gcaatacatc aggctcactg ccgtcccacg aaaacgtggc cccgcggctt cagacgtcag
     7621 ggcgataaga ttcggcaacg ccgcccccag cccgacaccg gtcatcagtc gcgcaaagac
     7681 cagtgagggg aaatcccagg caatcgccgt tgccagtgag aacaaaccaa acagcgcaac
     7741 tgagctaatt aaaatacgct tgcgaccata acggtccgcc agcattccgc caaccaacgc
     7801 gccgggtagc aaaccgagta ttccggcgct gaatatccag cccatttgca ttttatcgag
     7861 tgcgaaagcc tgggcgatgc cacccgccgc aatgccagcc gcctgaagat ccagtccttc
     7921 catcagagcg accaaaaaac aaagcccgat ggtcagcatc aggcgggatg aagatgatga
     7981 aggggtacga gtcgacataa gatgaacctc attgcgagtg caggtgaatc gcaactgata
     8041 cattgccagc cgcgtaatca gaactattta atgactgcct acgggcgaaa tgtaggccgg
     8101 ataaggcgtt cacgccgcat ccggcagtcg tgctgggatg ggcatacaaa atgcacgttc
     8161 tcggcagtca atattatttg ttgttgcgca gatccagcgc cacgtcgacg atcatatcct
     8221 cctggccgcc aaccatccgg cgtttgccca gctcaacgag aatatccacc gcgcttaagc
     8281 cataacgcgc cgccgccgtt tcacagtgac gcaggaagct ggagtaaaca ccagcgtatc
     8341 ccag
//
LOCUS       DAZGJT010000154         8308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZGJT010000154 DAZGJT010000000
VERSION     DAZGJT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.157"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..674
                     /locus_tag="ACHKFK_004370"
     CDS             <1..674
                     /locus_tag="ACHKFK_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO9054575.1"
                     /translation="YNRLFIPRQKTKDNNGLKTLNSKAVGRLLAAGGIYNGNIEGFRE
                     TANKLGGDAPAGYDQVMDNKGLLIAGASIVAGVVLGRMRFPDLESFEHFGARGAVSGR
                     PFNPDLAGGPIENLTIDGVTINREGIAIVEKHISRFDHDPANDVMISRLKKIANKELL
                     PEKYDLNYYTHECREYQRYCNLGWETGEPNGLDGYELWNNAHTATLEDFKIKDTDLFH
                     PDAKK"
     gene            671..928
                     /locus_tag="ACHKFK_004371"
     CDS             671..928
                     /locus_tag="ACHKFK_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9054576.1"
                     /translation="MTITIVEFNVLKVMAEKDIDWSWMVLDRTLAIRNIPGFGNVANI
                     VTKLVNHGLVDIVNGEGNSKPRYRVSQYGLNLIKEQQDNLF"
     gene            1003..1248
                     /locus_tag="ACHKFK_004372"
     CDS             1003..1248
                     /locus_tag="ACHKFK_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFO9054577.1"
                     /translation="MITKNELNVLNVMTEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVSEKGYNLVQRENK"
     gene            complement(1417..3678)
                     /gene="metE"
                     /locus_tag="ACHKFK_004373"
     CDS             complement(1417..3678)
                     /gene="metE"
                     /locus_tag="ACHKFK_004373"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO9054578.1"
                     /translation="MTILNHTLGFPRVGLRRELKKVQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHKVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDTLNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            3796..4749
                     /gene="metR"
                     /locus_tag="ACHKFK_004374"
     CDS             3796..4749
                     /gene="metR"
                     /locus_tag="ACHKFK_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO9054579.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            complement(4637..5536)
                     /gene="bioP"
                     /locus_tag="ACHKFK_004375"
     CDS             complement(4637..5536)
                     /gene="bioP"
                     /locus_tag="ACHKFK_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO9054580.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWIGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALNE"
     gene            complement(5612..6412)
                     /gene="yigL"
                     /locus_tag="ACHKFK_004376"
     CDS             complement(5612..6412)
                     /gene="yigL"
                     /locus_tag="ACHKFK_004376"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO9054581.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPEII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFNYALYEPGLLEPEGVSKVFFTCDSHEKLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLQDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNAEDAVPHYLRKLYLS"
     gene            complement(6420..7442)
                     /gene="pldB"
                     /locus_tag="ACHKFK_004377"
     CDS             complement(6420..7442)
                     /gene="pldB"
                     /locus_tag="ACHKFK_004377"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO9054582.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLGDP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEECVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7553..8173
                     /gene="rhtB"
                     /locus_tag="ACHKFK_004378"
     CDS             7553..8173
                     /gene="rhtB"
                     /locus_tag="ACHKFK_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO9054583.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            complement(8234..>8308)
                     /locus_tag="ACHKFK_004379"
     CDS             complement(8234..>8308)
                     /locus_tag="ACHKFK_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO9054584.1"
                     /translation="LAKWIDGFAGALFAGFGIHLIISR"
BASE COUNT         2145 a         2171 c         2052 g         1940 t
ORIGIN      
        1 actacaaccg tctgtttata ccacggcaaa aaactaaaga taataatggc ctcaaaacac
       61 tcaatagtaa agcggttggc agactgcttg ctgctggcgg catatacaat gggaatatcg
      121 aaggattcag ggaaacagca aacaagttgg gaggagatgc acctgctggt tacgatcaag
      181 ttatggataa caaagggtta ctcatcgctg gtgcttcaat agttgctgga gttgttctgg
      241 gaagaatgag attccctgat ttggaatcat ttgagcattt cggagccaga ggagcagtat
      301 caggaagacc atttaatccc gaccttgccg gtggtccaat agaaaacctg acgatagatg
      361 gcgtaacaat caatagggaa ggtatcgcta ttgttgaaaa gcatatctcc agatttgatc
      421 atgatccagc taatgatgta atgatttcaa ggctaaaaaa aatagcaaat aaagaattgc
      481 ttcctgaaaa atacgatctg aactactata ctcatgaatg ccgcgagtat cagcgttatt
      541 gtaatttagg atgggaaaca ggtgaaccga atggtttaga tggatatgaa ttatggaata
      601 atgcacatac tgcaactcta gaagacttta aaattaaaga tactgactta tttcatccgg
      661 atgcaaaaaa atgacgatta cgatagttga atttaacgtg ctgaaagtta tggctgagaa
      721 agatattgac tggagctgga tggtgctgga tcgtacttta gctatacgaa atattccagg
      781 atttggcaac gtagcgaata tcgtaacaaa gctggttaat catgggttgg ttgatatcgt
      841 aaatggtgag ggcaactcta aaccacgcta cagagtttct caatatggat tgaatcttat
      901 caaagaacaa caagacaacc tcttctgatc cagccattac aggagcatga tttacattgt
      961 gatctttcaa taagatactc atcaattttg gtacagaata tcgtgatcac taaaaatgaa
     1021 cttaacgtgc tgaacgttat gactgagaaa gatataaact ggaactggat gaatctcgat
     1081 cgcacactat ccataaggca aataccagga ttcggtaatg ttgttaatat tgttaacaaa
     1141 ttagctaacg aaggtttggt tattattgag gatagcgggc ataagtctat gcctcactac
     1201 cacgttagtg aaaagggata caatctcgtg caacgtgaga ataaataacg tagcgttgat
     1261 tactgaacgg aatcactcaa aactgtgttt tgcgaaaaaa acgattcggt tatttctact
     1321 cctgaagatc tgcttcaatc tggcactcat gcaaaatggc ttccagtact ggaagccatt
     1381 atcgtactgc aatttacaat gactcgttaa tactcatcat ccccgacgca aattctgcgc
     1441 cgcctgcacc atgttcgcca gtgccgcgcg ggtttctggc cagccgcgcg ttttcaggcc
     1501 acagtctggg ttgacccaca gacgctctgc cggaatgcgt tttgccgctt tcttcagcaa
     1561 ggcttcaatc cattccacgc tcggtacgtt tggcgagtga atgtcataga cgccaggacc
     1621 gatttcattt ggataatcaa actcttcaaa cgactccagc aactccatgt cggaacgcga
     1681 ggtttcgatg gtgatgacgt ctgcgtccag cgccgcaatc gaatccatga tgtcgttgaa
     1741 ctcgcagtaa cacatgtgag tgtggatttg tgtgtcatct ttcgccacgg cggcgttgat
     1801 acggaaagcc tccacgcccc actggagata ggcatcccaa tcgctgcgac gcagcggtaa
     1861 accttcgcgc aatgccggtt cgtcaatctg aatgatgcca attccagcgg cttccaggtc
     1921 cgcgacttca tcacgcagcg ccagcgcaat ttgtttggcg atggtttcac ggctgacatc
     1981 ttcacgcggg aacgaccagc agagaatagt caccgggccg gtcaacatcc ctttcaccgg
     2041 tttatcagtc agggattgcg catattttgc ccactccacg gtaatcggcg ccggacgact
     2101 aacgtcacca ataacaatcg gtggcttcac gcagcgggaa ccgtagctct gtacccaacc
     2161 gttttgcgta aagacaaacc cgtcgaggtg ctcgccaaag tattccacca tgtcattacg
     2221 ctcggcctcg ccatgtacca gcacatccag tcccaaacgt tcctgctcaa caatggcctg
     2281 cttgatatgt tccgcaatgc ccgtacggta gttattggcg tcgagattac cctttttgaa
     2341 atccagacgc agggtacgaa tctccgtggt ttgcgggaag gaaccaatcg tggtggtcgg
     2401 ccacgcgggc agtttaaaac gcgcacgctg ggcttcagca cgcacttcat agacattcgc
     2461 acgctgactg tcctgagcgg tgatcgccgc cagacgcttt tctactgccg gattatgtac
     2521 acgagtagag tgacgacgcg cctgaatcgg agcgctccac tctgccagag ctgccgtatc
     2581 accactgttc agcgtatcgc gcagcaatgc cagttcatga catttttgca gggcgaaggc
     2641 aaaccagctt ttcacttctg catcaagacg cgtttccacg ctcaggtcga tggggctgtg
     2701 cagcaacgag caggaagatg ccacccacaa atcgcgtttg ccgacaatgt ccttaatttg
     2761 cgcatatttc tcggtaagat cggcgcgcca gacgttacga ccattgataa gacccgcaga
     2821 cagcagccag tcagaaggca gacgcttgtg cagttcagca acgtcatctt taccatgtac
     2881 gagatcgaca tgcagaccct gaacaggcag cgcagtaatc gtgtcgaggt ttggcgttac
     2941 gccttcaaaa taggtagtca gcagcagttt cacctgcccc tgcagagcat cgtaagcggg
     3001 tttgtacgca tccagccacg cctgcggcag ttccagaacc agcgcgggtt catcaatctg
     3061 tacccactcg atgccgcgtt ttgccagttc tgccagcact tgctgataaa ccggcagaat
     3121 gtcgttcagc aggctcaggc gatcaaactg ttcacctttc actttaccca gccacaggta
     3181 ggtaaccggc cccagcagca cgggcttgac cttgtggccc agcgccagcg cctcgtccac
     3241 ttcctccagc agctgcgtcc aggtcagttt gaactgttgg cctttaacga actccggcac
     3301 catgtagtga tagttggtgt taaaccattt ggtcatttcc gctgccgccg caggttcgcc
     3361 agtcggtgca cgtccacgac caatacggaa cagggtgtcg atatctaccg aaccatcttt
     3421 gttctgatga cgtggcggaa cattacccag cagcagactg gtggtcagta catgatcgta
     3481 ccaggcaaaa tcgcccaccg gcagcaggtc gatacccgct tgcttttgtt gatcccagtg
     3541 acgagcacgt aattcacgcc ctaccgccag cagttcttca cgcgtggagt tccccgccca
     3601 ataactctct tgcacttttt tcagctcgcg acgcaggcca acgcgaggga aaccgagggt
     3661 gtgattaaga attgtcatta tatgcccctt atggaatttt tagtatttag ccgtccagat
     3721 gtttacacat ccataattgg cggttactgt atattcctca agcgcaaatt attcatgccg
     3781 aagtgaagga ctttcatgat cgaagtaaaa cacctgaaaa cgctacaagc gttgcggaac
     3841 tgcggctcac tcgcagccgc tgcggcgacg ttgcatcaaa cgcaatccgc cctgtctcac
     3901 cagtttagcg atctggaaca acgccttggc ttccggctat ttgtgcgtaa aagccagccg
     3961 cttcgcttta caccgcaggg agaaatcctg ttgcaactgg caaatcaggt gctgccgcaa
     4021 attagccagg cactgcaagc ctgcaatgaa ccgcagcaga cgcgcctgcg cattgccata
     4081 gagtgccata gctgtattca gtggctgacg cccgcattag aaaatttcca taagaactgg
     4141 ccgcaggtgg agatggattt taaatcgggc gtaacgtttg atccgcagcc tgcgttgcaa
     4201 cagggcgagc tggatctggt gatgacgtcc gatattctgc cgcgcagtgg cctgcattat
     4261 tcgccgatgt tcgactatga agtacgtctg gtgttagcac ctgaccatcc actggcggca
     4321 aaaacgcgta ttacaccgga agatctcgcc agcgaaacgc tgttgattta tccggtgcag
     4381 cgtagccgac tggatgtctg gcggcatttt cttcagccag caggcgtcag cccgtcactg
     4441 aaaagcgtcg ataacacctt attgttgatt cagatggttg ccgcacggat gggtattgcc
     4501 gcactaccgc attgggtggt ggagagtttt gaacgccagg gtctggtggt caccaaaacc
     4561 ctgggcgaag gcttgtggag ccgactgtac gccgccgtgc gcgatggcga gcagcgtcag
     4621 ccgattacag aagcgtttat tcgttcagcg cgcaatcacg cctgcgatca tctgccgttt
     4681 gtgaagagcg cggagcgacc cacttacgat gcacccacag tgaggccagg atcaccagcg
     4741 cgcctgtaat aaacgttggc cagtgcggct gttggtgcca gatagccagg ttaaccaaca
     4801 gccctgccgg aacgtgcata ttattcataa tgcccagcgt tccggcgtcc acctgcgtcg
     4861 cgccgtagtt ccacataaag tagccaatac cggaagccac cacgccaaga aacaccagaa
     4921 tgccccattg cagcgtggtt tgcggcattt tctgcgcatt tcccagcaag aaccatgcaa
     4981 tcactgccac caggaacgcg ccaagataga accacgcaaa ggcgttatgc tgcggcatcg
     5041 ggcgagtttc catcagacgt ttgtaaccca ccatgccgat ggcaaaagtg atattggaga
     5101 gttgcaccag caataagcca atccagaaat ggtcggtgac ctgatcatag cgaataatcc
     5161 cggcaccaat caccgccagc aaggcgctaa aggcatagcc ccagcgcaga cggcgcttac
     5221 tcatgatgtc ataaatcagc gtgatgtaga gcggcgtcag tacggtgaac aacagcagtt
     5281 cagaaaccgt caggtagaga taggcgcgga aactcagcat atacatcaca ccgagctgca
     5341 tcgcgcccac cagcatatac aggccgaccg ttttcaggct attgccacgg gtacgcagaa
     5401 acggcagaaa aacgagtgcc gccaggccaa cgcgcaccag caccgcaaaa tagctatcga
     5461 cgtgccccgc aaggtactcg ccataaaagc taaaggagaa ggcccacaga atcgtggtga
     5521 tgataagtag cgccacaatg gaaaactctt tgattaacga gtatccattg tagcgaagta
     5581 tcaggttgac aactgaccaa ataaagaacg attacgataa atagagctta cgcagataat
     5641 gcggcactgc gtcttcggcg ttagtaccaa tcacttccag ctctggatgc agatctttca
     5701 gccgttgatg cgcgctgccc ataatgcagc ctttccccgc catcgacagc atttcggcat
     5761 cgttcattcc atcgccaaag gcaatacaat cttgcaggct gtagcccagt ttcttcgcca
     5821 ccgcttccag cgcatggcct tttgaaacgc cgcccgccat cacttccagg caggttaagg
     5881 tagagaaact gacattgacg cgatctcccc agcgagcgtt aatcgcctgc tccagtggca
     5941 gaagtttttc atgggaatcg caggtgaaga acactttgct gacgccttcc ggctccagca
     6001 atcccggctc atacagcgca taattgaaca ccgcttcttt gaaaaagcgc atctcttccg
     6061 ggcgatggcg attcataaac cattcatcat cgcgataaac gttagtaatg atctccgggt
     6121 tgtcgttgac cacgccaaac aggtcactgg caatgtcgcg atccaggtta tgagcaaaaa
     6181 tcaggttgcc atccagatcg tgaacgcgcg cgccattgga ggtaatcatg taagacttaa
     6241 tctccagatt atcgcgaatt tgccccacat caacgtggtg acgaccggtc gcaaacacaa
     6301 agttgatgcc gcgcgcggtg agcagcttca gagtttcttt ggcgtaaggg gataacgtat
     6361 ggtcgggaga aagtaacgtg ccatctaaat cagacgcaac aacctggtac ataagaaatt
     6421 taaacctctg tagagcggtt tccgctgggt gagttatgcc tgttgaaaaa atcaacgata
     6481 gcgtggagcg cgactgagcg cattgcgtcc ttttcaaaaa ggatctcatg gtacgcacct
     6541 ttaattacca acggccgtcc tccttcgaca ggatggcccg cagcggtgcg tagttcacaa
     6601 aaacggtcgt gcatgcggtt atccaccacg cattcctctt cagcttgtaa gagaagcgtt
     6661 ggcgtggcgt catcacccgc accggctaac acctgttcgc cagccagaat actttcgcgt
     6721 acccaatggt aggtcggccc accgacgcga atcgttgggt catcagcata gaaacgtaag
     6781 ttacgtcgat atcgctgtct gctgtgggtc agtacgttga tagcaaacgg caacgcgcgc
     6841 cagcgcccgg tgcctattgc atagccatca cggaaacgtg gatgcgcttc ggcccaattg
     6901 aggatctgcc gtgccataaa tgacggcata cgaatcacga tcccaaacat tggcgcagtt
     6961 agcgcaatgg cgtcacatac acctggatgg cgttgcagaa ataatgtgga gatcgcaccg
     7021 cccatcgaat gtgccagtat atagcgttta cgccacggac cgggctgaac ctcctgttgc
     7081 cagaatgccg ccagatcatc aacataatca ttaaagcgat taacatgccc gagatgtgga
     7141 tcgcctaaca ggcgaccgga acgtccctgc ccgcgatggt cgatgattaa aacatcaaac
     7201 cccaaatgga acaggtcgta ggccagttcc gcatatttta cgtagctctc aatacgcccc
     7261 ggacagatga ctaccacccg gtcatggtgc tgtgcgcgaa aacggacaaa gcgcaccgga
     7321 atgtcatcca caccagtaaa ctctgcttca tcacgctgac gccagaaatc agtcagcggt
     7381 cccatggtaa aagcagcaaa cgcgttttct cttgtttccc agtctttttg ctgctgaaac
     7441 atcgggtaat ctgcctctta aaccacgtaa aatcgttttt ttaagcgtgc ctgacacaac
     7501 gctgcgacag tagcgtattg tggcacaaaa atagacacac cgggagttca tcatgacctt
     7561 agaatggtgg tttgcctacc tgctgacatc gatcatttta agcctgtcgc caggctctgg
     7621 cgcaatcaac accatgacca cctcgctcaa ccacggttat cgcggcgcgg tggcgtctat
     7681 tgctgggctt cagaccggac tggcgattca tattgtgctg gttggcgtgg ggttggggac
     7741 gctattttcc cgctcagtga ttgcgtttga agtgttgaag tgggcaggcg cagcttactt
     7801 gatttggctg ggaatacagc aatggcgcgc cgctggcgca attgatctta aatcgctggc
     7861 ctccactcaa tcgcgtcgac atttgttcca gcgcgcagtt tttgtcaatc tcaccaatcc
     7921 caaaagtatt gtgtttctgg cggcactatt tccgcaattc atcatgccgc aacagccgca
     7981 gctgatgcag tatatcgtgc tcggcgtcac cactattgtg gtcgatatta ttgtgatgat
     8041 cggttacgcc acccttgctc aacggattgc tctatggatt aaaggaccaa agcagatgaa
     8101 ggcgctgaat aagattttcg gctcgttatt tatgctggtg ggagcgctgt tagcatcggc
     8161 gaggcatgcg tgaaaataat gtcggatgcg gcgtaaacgc cttatccgac ttactctgat
     8221 gacgcgtctg gcattaacgc gaaataatca aatgaatgcc aaatccggca aataacgccc
     8281 cggcaaaacc atcaatccac ttcgccag
//
LOCUS       DAZGJT010000155         8257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZGJT010000155 DAZGJT010000000
VERSION     DAZGJT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.158"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..1090
                     /gene="rluB"
                     /locus_tag="ACHKFK_004380"
     CDS             215..1090
                     /gene="rluB"
                     /locus_tag="ACHKFK_004380"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO9054585.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNNG"
     gene            complement(1130..1720)
                     /gene="cobO"
                     /locus_tag="ACHKFK_004381"
     CDS             complement(1130..1720)
                     /gene="cobO"
                     /locus_tag="ACHKFK_004381"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO9054586.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPVKHAFDAGVKAQIGIDY"
     gene            complement(1717..2475)
                     /locus_tag="ACHKFK_004382"
     CDS             complement(1717..2475)
                     /locus_tag="ACHKFK_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO9054587.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIAVNYPRLDGVLHNAGL
                     LGDVCPMSEQDPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            2695..3744
                     /gene="sohB"
                     /locus_tag="ACHKFK_004383"
     CDS             2695..3744
                     /gene="sohB"
                     /locus_tag="ACHKFK_004383"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO9054588.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDTHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVVTDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(3780..4031)
                     /locus_tag="ACHKFK_004384"
     CDS             complement(3780..4031)
                     /locus_tag="ACHKFK_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO9054589.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            4411..7008
                     /gene="topA"
                     /locus_tag="ACHKFK_004385"
     CDS             4411..7008
                     /gene="topA"
                     /locus_tag="ACHKFK_004385"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO9054590.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCSKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            7218..8192
                     /gene="cysB"
                     /locus_tag="ACHKFK_004386"
     CDS             7218..8192
                     /gene="cysB"
                     /locus_tag="ACHKFK_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO9054591.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         2109 a         1947 c         2189 g         2012 t
ORIGIN      
        1 aataaagctg atgttaatcg aaacaagctt taaccataaa gatagtgtag tatgttgcgc
       61 ctcgaagcca ggccagttaa aacgtcgagt cgtttactta aggcctgaag agttcaaaca
      121 gactttacta tcagtgagtc agatgtaagt tctgactcat tgttgcacaa aagcatatca
      181 gacgcctggg aaggcgacac caaaaggaag ctctatgagc gaaaagctac agaaagtgct
      241 ggcgcgtgcc ggccacggtt ctcgccgtga aatcgaatct attattgaag ccggtcgtgt
      301 gagtgttgat ggcaaaattg ctaaactcgg cgatcgcgtt gaagttaccc ctggattgaa
      361 aatccgtatc gatggtcacc tgatttcggt acgtgagtcc gctgaacaaa tttgtcgcgt
      421 tctggcctat tacaaaccgg aaggtgagtt gtgtacccgt aacgatccgg aaggacgtcc
      481 aacggtgttt gaccgtctgc caaaactgcg tggcgcacgc tggattgccg tgggtcgcct
      541 ggacgttaat acctgtggtc tgctgctgtt caccaccgat ggtgaactgg caaaccgttt
      601 aatgcaccca agccgtgaag ttgaacgtga atatgccgtg cgtgtatttg gtcaggttga
      661 cgacgcgaaa ctgcgtgatt tgagtcgtgg cgtgcagttg gaagatggtc cggcagcctt
      721 taaaaccatc aagttcagcg gcggcgaagg gatcaaccag tggtacaacg ttactctgac
      781 cgaaggccgt aaccgtgaag ttcgtcgtct ttgggaagcg gttggtgtgc aggtgagccg
      841 cctgattcgt gttcgttacg gtgatatccc actgccaaaa ggtctgccac gcggtggctg
      901 gacggagctg gacctcgccc agactaacta tctgcgcgaa ctggtggaac taccgccaga
      961 aaccagctct aaagtcgctg tagaaaaaga ccgtcgtcgt atgaaggcga atcagattcg
     1021 tcgtgcggtg aaacgtcaca gtcaggtgag cggcggtcgt cgttctggcg gacgtaataa
     1081 taacggttaa tcaaagtatg ccggacgtca tatccggcat ttttacagat tagtaatcaa
     1141 tccctatctg cgcttttaca ccggcatcaa acgcatgttt gacggggcgt aattcactta
     1201 ccgtatccgc cagttcaaga atatcccgat gacaaccacg acctgtgata atcaccgtct
     1261 gttgatgtgg acgttcattt aacgcctgca ccacttcttc cagtggcaaa tagtcatacg
     1321 ccaccatata cgtcagttca tcgagcaaaa ccatatccag tgaggaatca gccagcatcc
     1381 gctttgcatg ttgccagact tcgcggcagg cggcggtatc agactctcgg ttttgtgtat
     1441 cccaggtaaa gcccgttgcc attacctgaa actcaacgcc gtgtggctcc agcagatttc
     1501 gttcaccatt aggccaggtg cctttaataa actgcacgac gcccactttt tttccgtgac
     1561 caactgcgcg tgttgccgta ccaaatgccg cggtggtttt gccttttcca ttgccggtaa
     1621 agacgatgat aataccgcgt tcatcctggg cctgggccac acgagcatct actttttctt
     1681 tcactcgctg ctgacgctgt tggtagcgtt catcactcat tgggaaattc ctggtttacg
     1741 gcccggttgg gcgtcaaagg tcatgccggt tttacggcgg ctgtcatcgc ccatcagcca
     1801 gaggtagagc ggcatgatat cagcgggggt tttaagtttc tgtggatctt cggtcgggaa
     1861 ggcgctggcc cgcattgcgg tgcgcgtacc gcctgggtta atgcagttga cacgcaggcg
     1921 ctgctgatat tcatcggcca gtacctgcat catcccttcg gtggcaaatt tcgacgctgc
     1981 atatgcgccc cagtttgcac gcccctgacg tccaacgctt gatgaggtaa agaccagtga
     2041 accggcatcc gatttgagta ataaaggaag cagtgcctgt gtgagcataa aggtggcatt
     2101 aacgttgacc tgcatgacgt cctgccagac ctgcggatct tgttcgctca ttgggcaaac
     2161 atcgccgagc aatccggcat tatgcaaaac accatccaga cgcggataat taacggcaat
     2221 gcgctgtgcc agttgttggc aatcttcgga cgtgcaggtt agcaaatcga gaataaacca
     2281 ctgtggctga cgtccagttt cttcgtttat gtggctggct acctgacgta atttttcttc
     2341 attacggccc aaaagaatca ctgtcgcacc atagcgtgca tacgtcatcg ctgcttcacg
     2401 accaattcca tcgctggctc ccgtcaccag gataatgcga tcattgagta aatcttgttt
     2461 tggctggtaa tgcatggcta ctcctcaacg acgttgtctg tcgcgctgcc agtgtacttt
     2521 atgactttgg ggctttatgc ctgaaacaga agggcatttc aatcagcctg gggcgataac
     2581 accgcaaaat ggatactttg tcatactttc gctgcaataa catctctgcg agacggctaa
     2641 acatgcctgt tgtaaactgt gagccaaagc gttgtttaac caaggtgggg actcgtggaa
     2701 ttgttgtctg aatatggttt gtttttggcg aaaatcgtta ccgttgtgct agcgattgcg
     2761 gcgattgccg ccattattgt caatgttgct caacgtaata aacgccagcg tggcgagtta
     2821 cgagtcaaca atctcagcga acagtataag gagatgaaag aagaactggc cgcggcgctg
     2881 atggacactc atcagcaaaa acagtggcac aaagcgcaga agaaaaagca caagcaagaa
     2941 gcgaaagccg caaaagcgaa agctaaactg ggcgaggtgg taactgacag taaaccccgc
     3001 gtctgggtgc tggattttaa aggcagcatg gacgcccatg aagtgaactc gctacgtgaa
     3061 gagataacgg cggtactcgc agcattcaaa ccgcaggatc aggttgtgct ccgtctggaa
     3121 agcccaggtg gcatggtgca tggttacggg ttggcggctt cgcagttgca gcgtctgcgt
     3181 gataaaaaca ttcctttaac tgttacggta gacaaagtcg ctgccagcgg cggttacatg
     3241 atggcctgtg tggcggacaa aattgtttcc gcaccgtttg ctattgtggg ttccattggg
     3301 gtggtggcgc aaatgcccaa ctttaaccgc ttcctgaaaa gcaaagatat tgatatcgaa
     3361 ctgcacaccg ccgggcagta taagcgcacg ctgacgttgc tgggtgaaaa taccgaagaa
     3421 gggcgggaga aattccgcga agagttgaac gaaacgcatc agttatttaa agattttgtg
     3481 aagcgtatgc gtccgtctct ggatattgaa caggtggcaa cgggtgaaca ctggtacgga
     3541 caacaggcag tagagaaagg cctggttgat gaaatcaaca ccagtgatga agttattctt
     3601 agcctgatgg aaggccggga agtggtcaat gtacgctata tgcagcgtaa acgactcatt
     3661 gatcgattca ccggcagcgc ggcagagagc gccgatcgat tgttgttacg ctggtggcag
     3721 cgtgggcaaa agccattgat gtaaaagaca aacgcgaggg taagacctcg cgttttgctt
     3781 taatcaacca gatgatattt ttctgaaagc acatgggcca ggtgtttgaa catattaaac
     3841 accgcggtgc ttttggcagt tggtaatcct tgttcatcta aaaagtagtc gccggtaaat
     3901 accagcacgc cattacgctg cgtgacgccg gtggcttcaa tccctgcgag cgtatcctca
     3961 tgctcacgaa tgattttgtt ggcctctttc aacagcgttt cgcggtcgat gggttgtgtc
     4021 tctttgttca ttatttactc cttaaacaag gacattagtc tacgccaggc atggcttgca
     4081 gacaaatata ccacgctggt ggcaagagcg ccttactggc aactttggat tttgcatgct
     4141 aataaagttg cgtatcggat tttatcaggt acagtgtgac gctttcgtca atctggcaat
     4201 agatttgctt gacattcgac caaaattccg tcgtgctata gcgcctgtag gccaagacct
     4261 gttaactcag tcacctgaat tttcgtgaac agagtcacga caaggggttg atatccgcag
     4321 agagcgagtc catatcggta actcgttgcc agtggaaggt ttatcaacgt gcgacgcatt
     4381 cctggaagaa tcaaattagg taaggtgaat atgggtaaag ctcttgtcat cgttgagtcc
     4441 ccggcaaaag ccaaaacgat caacaagtat ctgggtagtg actacgtggt gaaatccagc
     4501 gtcggtcaca tccgcgattt gccgaccagt ggctcagctg ccaaaaagag tgccgactct
     4561 acctccacta agacggctaa aaagcctaaa aaggatgaac gtggcgctct cgtcaaccgt
     4621 atgggggttg acccgtggca caattgggag gcgcactatg aagtgttgcc tggtaaagag
     4681 aaggtcgtct ctgaactgaa acaactggct gaaaaagccg accacatcta tctcgcaacc
     4741 gaccttgacc gcgaagggga agccattgca tggcacctgc gggaagtgat tggtggtgat
     4801 gatgcgcgct atagccgagt ggtgtttaac gaaattacta aaaacgcgat ccgccaggca
     4861 tttaacaaac cgggtgagct gaatattgat cgtgttaatg cccagcaggc gcgtcgcttt
     4921 atggaccgcg tggtggggta tatggtttcg ccgcttctat ggaaaaagat cgctcgtggt
     4981 ctgtctgccg gtcgtgtgca gtcggtggcg gtccgcctgg tggtcgagcg tgagcgtgaa
     5041 attaaagcgt tcgtaccgga agagttctgg gaagtcgatg ccagcacgac cacgccatct
     5101 ggtgaagcgt tggcgttgca ggtgactcat cagaacgaca aaccgttccg tccggtcaac
     5161 aaagaacaaa ctcaggctgc ggtaagtctg ctggaaaaag cgcgctacag cgtgctggaa
     5221 cgtgaagaca aaccgacaac cagtaaacct ggcgctcctt ttattacctc tacgctgcaa
     5281 caagctgcca gcacccgtct tggttttggc gtgaaaaaaa ccatgatgat ggcgcagcgt
     5341 ttgtatgaag caggctatat cacttacatg cgtaccgact ccaccaacct gagtcaggac
     5401 gcggtaaata tggttcgcgg ttatatcagc gataattttg gtaagaaata tctgccggaa
     5461 agtccgaatc agtacgccag caaagaaaac tcacaggaag cgcacgaagc gattcgtcct
     5521 tctgacgtca atgtgatggc ggaatcgttg aaggatatgg aagcagatgc gcagaaactg
     5581 taccagttaa tctggcgtca gttcgtagcc tgccagatga ccccagcgaa atatgactcc
     5641 acgacgctga ccgttggtgc gggcgatttc cgcctgaaag cacgcggtcg tattttgcgc
     5701 tttgatggct ggacgaaagt gatgcctgca ctgcgtaaag gcgatgaaga tcgtatctta
     5761 ccggcagtcg ataaaggcga tgctctgacg ctcgttgaac tgacaccagc ccagcacttt
     5821 accaagcctc cagcccgttt cagtgaagca tcgctggtca aagagctgga aaaacgcggt
     5881 atcggtcgtc cttctaccta tgcgtcgatc atttcgacca ttcaggatcg tggctatgtg
     5941 cgagtagaaa atcgtcgttt ctatgcggaa aaaatgggcg aaatcgtcac cgatcgcctg
     6001 gaagagaatt tccgcgagtt aatgaactac gacttcaccg cgcagatgga aaacagcctc
     6061 gaccaggtgg caaatcacga agcagagtgg aaagctgtac tggatcactt cttctcggat
     6121 ttcacccagc agttagataa agctgaaaaa gatccggaag agggtggtat gcgcccgaac
     6181 cagatggttc tgaccagcat tgactgtccg acttgtggtc gcaaaatggg gattcgcaca
     6241 gcgagcaccg gggtattcct tggctgttct ggctatgcgc tgccgccgaa agagcgttgc
     6301 aaaaccacca ttaacctggt gccggaaaac gaagtgctga acgtgctgga aggtgaagac
     6361 gctgaaacca atgcgttgcg cgcaaaacgt cgttgctcga aatgcggcac ggcgatggac
     6421 agctatctca tcgatccgaa acgtaagttg catgtctgtg gtaataaccc aacctgcgac
     6481 ggttacgaga tcgaagaggg tgaattccgc attaaaggtt atgacggccc gatcgttgag
     6541 tgtgaaaaat gtggctctga aatgcacctg aaaatggggc gtttcggtaa atacatggcc
     6601 tgcaccaacg aagagtgtaa aaacacacgt aagattttac gtaacggcga agtggcacca
     6661 ccgaaagaag atccggtgcc attaccggag ctgccgtgcg aaaaatcaga tgcctatttc
     6721 gtgttgcgtg acggtgctgc cggtgtgttc ctggcggcca ataccttccc gaaatcgcgt
     6781 gaaacgcgtg cgccgctggt ggaggagctt tatcgcttcc gcgaccgtct gccggaaaaa
     6841 ctgcgttatc tggccgatgc gccacagcag gatccggaag gtaataagac catggttcgc
     6901 tttagccgta aaaccaaaca gcaatatgtc tcttcggaaa aagacggaaa ggcgactggc
     6961 tggtcagcat tttatgttga tggcaaatgg gttgaaggga aaaaataacc tttaattctg
     7021 tcgggttttt ataaacaaag ggccgcgaaa gcggcccttt tttattgcat attatttttt
     7081 cttcacacct atacactaag gctataaatg atatagtggt tatagttagc acctttttta
     7141 ttattaaatc gtattagtca cccgcctggt gtgacgaaaa aacgatgttc tgatggcgtc
     7201 taagtggatg gtttaacatg aaattacaac aacttcgcta tattgttgag gtggtcaatc
     7261 ataacctgaa tgtctcatca acggcggaag gactttacac atcacaaccc gggatcagta
     7321 aacaagtcag aatgctggaa gacgagctag gcattcaaat tttttcccga agcggcaagc
     7381 acctgacgca ggtaacgcca gcaggacaag aaataattcg tatcgctcgt gaagttctgt
     7441 cgaaagtcga tgccataaaa tcggtcgccg gagagcacac ctggccggat aaaggctcgc
     7501 tgtatatcgc caccacgcat actcaggcac gctatgcgtt accaaacgtt atcaaaggct
     7561 ttattgagcg ttatcctcgc gtttctttgc atatgcacca gggctcgccg acacaaattg
     7621 ctgatgccgt ctctaaaggc aatgccgatt tcgctatcgc cacggaagcg ctgcatctgt
     7681 atgaagattt agtgatgcta ccgtgctacc actggaatcg ggctatagta gtcactccgg
     7741 atcacccgct ggcaggtaaa aaagccatta ccattgaaga actggcgcaa tatccgttgg
     7801 tgacatatac cttcggcttt accggacgtt cagaactgga tactgccttt aaccgcgcag
     7861 ggttaacgcc gcgtatcgtc ttcacggcaa cggatgctga cgtcattaaa acttacgtcc
     7921 ggttagggct gggggtaggg gtcattgcca gcatggcggt ggatccggtc gccgatcccg
     7981 accttgtgcg tgttgatgct cacgacatct tcagccacag tacaaccaaa attggttttc
     8041 gccgtagtac ttttttgcgt agttatatgt atgatttcat tcagcgtttt gcaccgcatt
     8101 taacgcgtga tgtcgttgat gcggctgtcg cattgcgctc taatgaagaa attgaggtca
     8161 tgtttaaaga tataaaactg ccggaaaaat aatttcttgc gttattttcg gcacctttta
     8221 tgtagcgaag gtgccggaat atattctcct ttgttac
//
LOCUS       DAZGJT010000156         8240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZGJT010000156 DAZGJT010000000
VERSION     DAZGJT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.159"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(16..675)
                     /locus_tag="ACHKFK_004387"
                     /pseudo
     CDS             complement(16..675)
                     /locus_tag="ACHKFK_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            969..1724
                     /gene="mlaA"
                     /locus_tag="ACHKFK_004388"
     CDS             969..1724
                     /gene="mlaA"
                     /locus_tag="ACHKFK_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO9054592.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADSLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(1786..3126)
                     /gene="fadL"
                     /locus_tag="ACHKFK_004389"
     CDS             complement(1786..3126)
                     /gene="fadL"
                     /locus_tag="ACHKFK_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO9054593.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNITGHSDLTGQSANAKNIAPT
                     AWVPNLHFVAPINDQFGWGASITSNYGLATEFTDNYAAGIYGGKTDLQTINLNLSGAY
                     RLNNSWSFGLGFDAVYAKAKIERYAGSLGPLLSQQLIAQGVPASLTNGINTPDSQIAH
                     LKGDEWGFGWNAGILYELDKDNRWGLTYRSEVKIDFDGDYKSSINPNLNNILGNMGLP
                     YGTMGATQNGSLTLNLPEMWEISGYNRVAPQWAIHYSMAYTSWSQFQELKAKGDSGQT
                     LFYKDEGFRDAYRIALGTTYYYDKNWTFRTGIAYDDSPVPADKRSISIPDQDRLWLSA
                     GLTYAFNEDASIDVGASYMHGQKVKFTEGEGAAAYSFESEGKAWLFGTNFNYAF"
     gene            3498..3782
                     /gene="yfcZ"
                     /locus_tag="ACHKFK_004390"
     CDS             3498..3782
                     /gene="yfcZ"
                     /locus_tag="ACHKFK_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO9054594.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            3963..5273
                     /gene="fadI"
                     /locus_tag="ACHKFK_004391"
     CDS             3963..5273
                     /gene="fadI"
                     /locus_tag="ACHKFK_004391"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO9054595.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARTEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     EGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFARDVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            5273..7417
                     /gene="fadJ"
                     /locus_tag="ACHKFK_004392"
     CDS             5273..7417
                     /gene="fadJ"
                     /locus_tag="ACHKFK_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO9054596.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QIRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEVLARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQAVKLGLVDDVVPHSILLEAAVELAKQDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARTFGEL
                     AMTPQSQALRNIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPRGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYCGFAHRDLI
                     IEAVFENLEIKQQMVAEVEQNCATHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHASTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERIEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIMPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCLDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCDRLVEMSERGESFWKTTATDLQ"
     gene            7620..8105
                     /gene="sixA"
                     /locus_tag="ACHKFK_004393"
     CDS             7620..8105
                     /gene="sixA"
                     /locus_tag="ACHKFK_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO9054597.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVGAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGKGQFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2011 a         1986 c         2142 g         2101 t
ORIGIN      
        1 cagggcaata agctaggtca tcaatataat caccacaatc tttgccgcac ccgctgcagg
       61 aaacatccat accatagtgg cgatcagcca gccggaaatg atcgcgttgg caaacatctc
      121 gctgggggtg ttcttcatca catccatgcc gattttggca aatgcatcgc gagtttcttc
      181 attgaagata ggcatatatt caaatgccca cgcagcaata cctgtcccga taatattacc
      241 cagcagcacg atgccccata accgcataag taagccgacg ttgctcattg tcggtttttg
      301 catgacgggt agtaccgcag ttacggtgtt ttcggtaaat aattgctggc gggccattat
      361 gacgataata aaaccaaagg tataaccgag attctccagc aagaagctgc ccggcacgcc
      421 ttccagttcg acatgaaata tcccttttgc cagtagcgaa gcgcccatcg acaggcccgc
      481 cgcaatggct gaccacagta gcgccattgc gtcgcgttcc agctcttttt caccatcctg
      541 gcggatatgc tcatgaattg ccatcgcccg ggaggggagt cggtcttcat ctatttctat
      601 ttttttaccg cgctcttttt cttcgctctc aacttcaatt tcgtcgctgt gttgatcaat
      661 tttgtcgttg tccatggtct cttctcttga attagcacgt atagctaaag cgtagcggct
      721 tttttgctcg caactggcgg gagttactct gaaaatgtag aaaaggccgc gtttgccttt
      781 ttctgtttct attgaatcaa gtagcctaca gggcggcgat taccaggcta tgatcaaatc
      841 agcaaatcag ggcgtctgga catcagttga cgtgctgtta caatcgccca cacctaaaca
      901 ggcggatacg gtatcgttcc gtcatggatg gcaaactgca taagccataa aaaaacaggg
      961 agacatttat gaagcttcgc ctgtcggcgc ttgctctggg aactacgctt ctggtggggt
     1021 gtgcgagttc cggtacagat cagcaagggc gttctgaccc gttagaaggg tttaaccgca
     1081 ctatgtacaa cttcaacttc aatgtattag acccgtatat tgttcgaccg gtcgctgtcg
     1141 cctggcgtga ttatgttccg caacctgcgc gtaacggttt gagcaacttt accggcaacc
     1201 ttgaagaacc tgcggtgatg gttaactact tcttgcaggg cgacccttat caggggatgg
     1261 ttcactttac ccgctttttc ctgaatacca ttttgggaat gggcggcttt attgatgttg
     1321 cagggatggc gaacccgaaa ctgcaacgga ctgaacctca ccgcttcggt agtacgcttg
     1381 gtcattatgg tgtgggttat gggccgtacg ttcagttacc gttctacggt agcttcacgc
     1441 tgcgtgatga cggtggtgat atggcggata gtctttaccc ggttctttcc tggctgacct
     1501 ggccgatgtc tgtgggtaaa tggacgcttg aagggataga aactcgtgcg cagttgttgg
     1561 attccgacgg tctgctgcgt cagtcctccg atccttatat tatggtgcgt gaagcgtact
     1621 tccagcgtca tgatttcatc gctaatggcg gtgaactcaa accgcaggaa aatccgaacg
     1681 cgcaagcgat tcaggatgat ttaaaagata tcgattctga ataagaaaca aacaaaaaag
     1741 gtgagtcgca aaactcacct tttttcttat taagctaacg cgttgtcaga acgcgtagtt
     1801 aaagttagta ccgaacagcc aggctttacc ttcagactcg aaggaataag ctgccgctcc
     1861 ttcaccttcg gtgaatttca ctttctgacc atgcatataa gatgcgccaa catcaatgga
     1921 tgcatcttca ttaaatgcgt aggttaaccc agcacttaac cacaaacgat cctggtcagg
     1981 aatggaaata gaacgtttat ctgccggaac cgggctgtcg tcataagcga taccggtacg
     2041 gaaggtccag tttttatcat aatagtaggt tgtacccaac gcgatacgat acgcgtcccg
     2101 gaatccttca tctttataga acagagtctg accactatcg cctttcgctt tcaattcctg
     2161 gaactggctc caactggtat aggccatgct gtagtgaatt gcccactgcg gcgcaacacg
     2221 gttataacca gaaatctccc acatctcagg caggttcagc gttaaggatc cattttgggt
     2281 tgcccccatc gtgccatacg gtaagcccat gttacccaaa atattgttca gatttgggtt
     2341 aatggagctt ttgtaatccc catcgaagtc gattttaact tctgaacgat atgtcagccc
     2401 ccaacggtta tctttatcca gttcataaag aatacccgcg ttccagccaa agccccattc
     2461 gtcacctttc aaatgagcaa tttgagagtc cggagtatta ataccattgg ttaatgaagc
     2521 gggtacaccc tgggcaatca actgttgaga caacaatggc cccagcgaac cggcgtaacg
     2581 ctcaattttt gccttggcat aaactgcatc aaaaccaaga ccaaaactcc agctattgtt
     2641 aaggcgataa gccccgctaa ggttaaggtt gattgtttgc agatcggttt taccgccata
     2701 gataccagca gcgtagttgt cggtaaattc cgtagccagg ccatagttgg aagttattga
     2761 cgctccccaa ccaaactgat cgttaatagg cgcaacaaag tgcaggttag gtacccatgc
     2821 tgtcggtgcg atatttttag catttgcact ctgaccggtt aaatcagaat gtccggtaat
     2881 atttacatcc ggatcaatat aaaccgcacc tgcagaaaat gtcgggcggt caaacatggt
     2941 aatcaatgcg gggttacggc taacgttacc tgcatcatcg gcaattgcac cttcccctga
     3001 ataagcccgg cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc
     3061 ctgggtggag ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg
     3121 gctcatgacc ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag
     3181 gagcgcgaag tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag
     3241 gtcggaccag ctggaatgtt gcaagtatgt ttctatcttt tttcagatat gatctaagaa
     3301 acgcgatcca gctggcaaaa ttgggaggtg gcgcactcat ggattcgggt gaattttgtt
     3361 ttagatcatt tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg
     3421 agcagcactt tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca
     3481 tacagaggaa atcaatgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta
     3541 tggatgttgg caccattatg gacaactccg actgcaccgc gtcttacagc cgtgtgtttg
     3601 caaaccgcgc agaagcagaa caaacgctgg cggcgctgac tgaaaaagcc cgtagcgtgg
     3661 aatccgaacc gtgcaaaatc accccgactt tcactgaaga gtctgacggt gtgcgcctgg
     3721 atatcgattt cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt
     3781 aattcctctc gggtacatac gcccctgtca gttgctggca ggggcgtttt cttttctctt
     3841 cttcttttct ctctgtgtca tagctcccac tttccctttt ctccacttgg ctaaatgtaa
     3901 aaaaatggtt aagactatga tcaggtcaga ccactttatt tattttttta caggggagtg
     3961 ttatgggtca ggttttaccg ctggttaccc gccagggcga tcgtatcgcc attgttagcg
     4021 gtttacgtac gccttttgcc cgacaggcga cggcttttca tggcattccc gcggttgatt
     4081 tagggaagat ggtggtaggc gaactgctgg cacgcaccga gatccccgct gaagtgattg
     4141 aacaactggt cttcggtcag gtcgttcaga tgcctgaagc ccccaacatt gcccgtgaaa
     4201 ttgttctcgg tactggaatg aatgtgcata ccgatgctta cagcgttagc cgcgcttgcg
     4261 ctaccagttt ccaggcggtt gcaaacgtcg cagaaagcct gatggcggga actattcgag
     4321 cggggattgc cggtggggca gattcctctt ccgtattgcc aattggcgtc agtaaaaaac
     4381 tggcgcgcgt actggttgat gtcaacaaag cccgcacaat gagccagcgc ctgaaacttt
     4441 tttctcgctt acgtttgcgt gacttaatgc cggtgcctcc ggcagtggcg gaatactcca
     4501 ctggattacg gatgggggac accgcagagc aaatggcgaa aacctacggc atcacccgag
     4561 aacagcaaga tgcactggcg caccgttcgc atcagcgtgc cgctcaggcg tggtcagaag
     4621 gtaaactcaa agaagaggtg atgactgcct ttatcccccc ttataaacaa ccgcttgtcg
     4681 aagacaacaa tattcgcggt aattcctctc ttgctgatta cgcaaagctg cgtccggcgt
     4741 ttgatcgtaa acacgggacg gtaacggcgg cgaacagtac gccgctgacc gatggcgcgg
     4801 cagcggtgat cctgatgact gaatcccggg cgaaagaatt agggctggta ccgctggggt
     4861 atctgcgcag ctacgcattt actgcgatag atgtctggca ggacatgttg ctcggtccag
     4921 cctggtcaac accgctggcg ctggaacgtg ccggattgac gatgagcgat ctgacattga
     4981 tcgatatgca cgaagccttt gccgcccaga cgctggccaa tattcagttg cttggcagcg
     5041 aacgttttgc tcgcgacgta ctggggcgtg cacatgccac tggcgaagtg gacgatagca
     5101 aatttaacgt gcttggcggt tcgattgctt atggacatcc cttcgcagcg accggcgcgc
     5161 ggatgattac ccagacattg catgaacttc gccgtcgcgg cggtggattt ggtttagtga
     5221 ccgcctgtgc tgcgggtggg cttggcgcag caatggttct ggaggcggaa taatggaaat
     5281 ggcatcagcg tttactctta atgttcgtct ggacaacatt gctatcatca ccatcgacgt
     5341 accgggtgag aaaatgaata ccctcaaggc ggaatttgcc tcgcaggtac gcgccattat
     5401 taagcaaatc cgtgaaaaca aagagttgcg tggcgtggtg tttgtctccg cgaaaccaga
     5461 caacttcatt gctggcgcag acatcaacat gatcggcaac tgcaaaacgg cgcaagaagc
     5521 ggaagtactg gcgcggcagg ggcaacagtt aatggcggag attcatgctt tgcccattcc
     5581 ggttatcgcg gctattcatg gcgcttgcct gggcggcggg ctggagttgg cgctggcgtg
     5641 ccacggtcgt gtttgtactg acgatcctaa aacggtgctc ggtttgcctg aagtacaact
     5701 tggattgtta cccggttcag gcggtaccca gcgtttaccg cgcctgatag gtgtcagcac
     5761 agcattagag atgatcctca ccggaaaaca acttcgggcg aaacaggcgg taaagttggg
     5821 gttggtggat gacgtcgttc cgcactccat attgctggaa gccgctgttg agctggcaaa
     5881 gcaggatcgc ccatcttcac gccctctacc tgtacgcgag cgtattctgg cggggccgtt
     5941 aggtcgtgcg ctgctgttca aaatggtcgg taagaaaaca gaacacaaaa cgcaaggtaa
     6001 ctatccggcg acagaacgca tcctggaggt tgtcgaaacg ggattagcgc agggcaccag
     6061 cagcggctat gacgctgaag cacggacgtt tggcgaactg gcgatgacgc cgcaatcgca
     6121 ggcgctgcgc aatatctttt ttgccagtac ggacgtgaag aaagatcccg gcagtgatgc
     6181 gccgcctgcg ccattaaaca gcgtggggat tctaggtggt ggcttgatgg gcggcggtat
     6241 tgcttatgtc actgcttgta aagcggggct tccggtcaga attaaagata tcaacccgcg
     6301 gggcataaat catgcgctga agtacagttg ggatcagctt gagggcaaag ttcgccgtcg
     6361 tcatctcaaa gccagcgaac gtgacaaaca gctggcatta atctccggaa cgacggacta
     6421 ttgcggcttt gcccatcgcg atctgattat tgaagcggtg tttgaaaatc tcgaaataaa
     6481 acaacagatg gtggcggaag tggagcaaaa ttgcgctact cataccatct ttgcttcgaa
     6541 cacgtcatct ttaccgattg gcgatatcgc cgctcacgct gcgcgacctg agcaagttat
     6601 tggcctgcat ttcttcagtc cggtggaaaa aatgccgctg gtggagatta ttcctcatgc
     6661 gagtacatcg gcgcaaacca tcgccaccac ggtaaaactg gcgaaaaaac agggcaaaac
     6721 gccaattgtc gtgcgtgaca aagccgggtt ttacgtcaat cgcatcttag cgccttacat
     6781 caatgaagct atccgcatgt tgaccgaagg cgagcgtatt gagcatatcg atgccgcgct
     6841 ggtgaaattt ggttttccgg taggcccaat ccaacttttg gatgaggtag gaatcgacac
     6901 cgggactaaa attatgcctg tactggaagc cgcttatgga gaacgtttta gcgcgcctgc
     6961 aaatgttgtt tcttcaattt tgaacgacga tcgcaaaggc agaaaaaatg gccggggttt
     7021 ctatctttat ggtcagaaag ggcgtaaaag caaaaaacag gtcgatcccg ccatttaccc
     7081 gctcattggc gcacaagggc aggggcgact ctccgcaccg caggttgctg aacggtgtgt
     7141 gatgttgatg ctgaatgaag cagtacgttg tctggacgag caggttatcc gtagcgtacg
     7201 tgacggggat attggcgcgg tatttggcat tggttttccg ccatttctcg gtggaccgtt
     7261 ccgctatatc gattctctcg gcgcaggcga agtggttgca ataatgcaac gactggccac
     7321 gcagtatggt tcccgtttta ccccttgcga tcgtttagtt gagatgagcg agcgtgggga
     7381 aagtttttgg aaaacaactg caactgacct gcaataagaa ggtcaaagtt atatgaatcc
     7441 gcgctgaatg gcggagtttt gctcaaaatg taaacgcata ttgactatac ttacgccatt
     7501 gaggtagaaa acagcgtttc attcggtgaa tggataaggc acaatgccgg ccaccgtttt
     7561 ctttctctgg tttcagatga aagaaaacgg gcgaatctgg ttaacaaaag cggtgcaata
     7621 tgcaagtttt tatcatgcgt cacggcgacg cagccctcga tgccgccagt gattcggttc
     7681 gtcctctgac cactaacggt tgtgacgaat ctcgcctgat ggcgaactgg ctgaaaggtc
     7741 aaaaagtgga aatcgaacgc gttctggtga gcccgttcct gcgagccgag caaacactgg
     7801 aagaagtagg ggattgtctg aacctgcctt ccagtgcaga agttctgccg gagttaacgc
     7861 cctgcggcga tgttggtctg gtcggcgcct atttgcaggc gttgaccaat gaaggtgttg
     7921 cttcggtact ggtgatctcc cacttaccgt tagtcgggta tctggttgcc gagttatgtc
     7981 cgggcgaaac gccgcctatg tttaccactt cagcgattgc cagcgtaacg cttgatgaaa
     8041 gcggaaaagg ccaattcaac tggcaaatga gcccttgtaa tttgaagatg gcaaaagcaa
     8101 tttaagcaat caatgcccgg ttacgtgcgc gtacaccggg cagtttctcc gtcatacaat
     8161 cattcttttt cttgtctaaa tgttgttaat atttaacaac tctcgatagt tattatattt
     8221 ttccattatt gaatcccgta
//
LOCUS       DAZGJT010000157         8042 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZGJT010000157 DAZGJT010000000
VERSION     DAZGJT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8042
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.160"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1930
                     /locus_tag="ACHKFK_004394"
     CDS             <1..1930
                     /locus_tag="ACHKFK_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO9054598.1"
                     /translation="DDAGFDEEDFKKPIRVHLPVVNDLPPEGVFDTEFCNRYEKGGND
                     GITMMAIPFDDNINGEDATTAGDDNDNLDGTIPDDVEKSESPDSDDDCSECEIPVATL
                     SLTHRFLHLFLFSKDEDGKYRHHATPEQRNNVIRMEMDTEDSYLQSLLTAVRAAHHEL
                     DKLTNYHLSRLAESVGKAFPHSANHRISPAEFDKFISTWMKTDYLDQGLLTKEWQNGN
                     YVSGITRTPSGANAGGGNITDRGEGFKHDKTSLARDVATGVLARSMDVDIYNLHPAHA
                     KRVEEIVSENKPPFSVFRDKFIAMPGGLDYSRAIVVASVKEAPIGIEAIPARVTEYLN
                     KVLTETDHANPDPEIVEIACGRSSAPMPQRGTAEGKHGDEEKQQASDTMANEQAAPES
                     VEEIPVKHNEDTQSLENVSSVETKYQELREELNKARENIPPKNPVDADKLLAASRGEF
                     VEGISNPADPKWVKGIQTRDTEDQNQSKVEQIAPEAGQNSPDTQQNGPEEQQPGPVAQ
                     PELEKNCRVCGQTGGGNCPDCSAVMGDSTYTETFGENDAADGEDSAQTEEKIIQENSV
                     DAAQEGETVVQNEPGSDTSGDDANSEPVTLDWKRQLVIAAVYGLCANPACIATAPAIP
                     DIAIMIANRLENFGGDKS"
     gene            1927..2223
                     /locus_tag="ACHKFK_004395"
     CDS             1927..2223
                     /locus_tag="ACHKFK_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO9054599.1"
                     /translation="MNAWLIPDRIEEQSWARHYQQIAREETEAELADDLEKGLPQHLF
                     ESLCIDNLQRHGASKKAISRAFDDDVDFQERMAEHIRYMAETIARHQINIDSEV"
     gene            2229..3014
                     /gene="bet"
                     /locus_tag="ACHKFK_004396"
     CDS             2229..3014
                     /gene="bet"
                     /locus_tag="ACHKFK_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9054600.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRRAPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTTERQPERDITPVNEETMSEINALLTSMEKTWDD
                     DLLPLCSQIFRRNIYTSSELTQAEAVKVLGFLKQKVTEQKVAA"
     gene            3011..3691
                     /locus_tag="ACHKFK_004397"
     CDS             3011..3691
                     /locus_tag="ACHKFK_004397"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO9054601.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMEVFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            3739..3990
                     /locus_tag="ACHKFK_004398"
     CDS             3739..3990
                     /locus_tag="ACHKFK_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO9054602.1"
                     /translation="MFVLIQRGQSFVDANNYPVQVCKVTLTQVIFRRLDGKTRAASIN
                     SFNAEFERIDHNELHMIKAEIEKEKHIASLRKMRRTSIN"
     gene            4036..4320
                     /locus_tag="ACHKFK_004399"
     CDS             4036..4320
                     /locus_tag="ACHKFK_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO9054603.1"
                     /translation="MREFVNQHSHGIQPVITPVVQINANEWVTLELLMAVTGLRKGTI
                     LRARDAAWMNGREYKQIAPDGTPKKNSECLYHLPTINTWIKNQPLPSQDV"
     gene            4373..5683
                     /locus_tag="ACHKFK_004400"
     CDS             4373..5683
                     /locus_tag="ACHKFK_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9054604.1"
                     /translation="MANSAYPAGVENHGGKLRITFKYRGKRVRENLRVPDTPKNRKIA
                     GELRASVCFAIRTGTFDYAERFPDSPNLKLFGLVKKDITVGELAQKWLTLKAMEISSN
                     ALNRYQSVMKNMLPRLGVGRLASSITKEDLLFIRKDLLTGEKESRKNTSTNKGRTVPT
                     VNYYMTTTAGMFSFAAENGYLEKNPFNSITPLRKSKPVPDPLTREEFGRLIDACHHQQ
                     TKNLWTVAVFTGMRHGEIAALAWEDIDLKVGTITVRRNFTKIGDFTLPKTDAGTDRVI
                     HLLAPAIEALKNQAMLTRLGRQHQITVKLREYGRTILHECTFVFCPQIVRKNHKAGIN
                     YAVSSIGATWDSAMKRAGIRARKAYQSRHTYACWALSAGANPTFIASQMGHSSASMVY
                     NVYGAWMPECSVTQVAMLNNVLNARAPDVPQSDQEDEIKLYFSK"
     gene            5680..6258
                     /gene="wrbA"
                     /locus_tag="ACHKFK_004401"
     CDS             5680..6258
                     /gene="wrbA"
                     /locus_tag="ACHKFK_004401"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000607019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO9054605.1"
                     /translation="MIKHILRHVETMARAVAEGASKVDGAEVVVKRVPETMPPQLFEK
                     AGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGALYGKLA
                     SVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPYGATTIA
                     GGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            6279..6506
                     /locus_tag="ACHKFK_004402"
     CDS             6279..6506
                     /locus_tag="ACHKFK_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO9054606.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(6544..7785)
                     /gene="agp"
                     /locus_tag="ACHKFK_004403"
     CDS             complement(6544..7785)
                     /gene="agp"
                     /locus_tag="ACHKFK_004403"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO9054607.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAVMENELSKLQLTDSYKLLEQIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSGEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
BASE COUNT         2240 a         1994 c         2000 g         1808 t
ORIGIN      
        1 ggacgatgcc ggatttgacg aagaggattt taaaaaaccc atccgcgtcc atttacccgt
       61 tgtgaatgac cttccaccgg aaggcgtgtt tgatactgaa ttctgcaacc gctatgaaaa
      121 aggcggtaac gatggcatca ccatgatggc tatccccttc gatgacaaca tcaacggtga
      181 agatgccaca actgctggcg atgacaacga taacctggac ggaactattc cggatgatgt
      241 ggagaaaagc gaatccccgg acagcgacga tgactgttct gagtgtgaaa ttcccgtcgc
      301 cactctgagc cttactcatc gcttccttca cctcttctta ttcagcaaag atgaagatgg
      361 aaaataccgg catcatgcca caccagaaca acgcaataac gtgatccgta tggagatgga
      421 cacagaggac agttaccttc agagcctgct tactgctgtg cgcgccgcgc atcatgaact
      481 ggataaactg acgaactatc accttagtcg cctggctgaa tctgtaggga aagcattccc
      541 ccactctgca aatcatcgca tcagcccggc tgaattcgac aagttcattt ccacctggat
      601 gaaaactgac taccttgatc agggcctgct gacaaaagaa tggcagaacg gaaattatgt
      661 ttcaggcatt acccgtacgc cttccggtgc taacgctggc ggcggaaata ttaccgatcg
      721 tggtgaagga ttcaaacatg ataagacatc actcgcacga gatgtagcca ccggcgttct
      781 ggcccgttca atggatgtgg atatttataa cctgcaccca gcacacgcaa aacgcgttga
      841 agaaatcgtg tcagagaata agccgccctt ttctgttttt cgcgacaaat ttatcgccat
      901 gcccggtggg ctggattatt cccgcgccat tgtggtggct tccgtgaaag aagcaccaat
      961 cggcattgag gccatcccgg cacgcgtgac tgaatatctc aacaaagtgt tgaccgaaac
     1021 cgatcacgct aacccggatc cggaaatcgt ggaaattgcc tgcggtcgct catcagcacc
     1081 gatgccgcag cgcggaacag cagaaggaaa acatggcgat gaagaaaagc aacaagcatc
     1141 ggacacaatg gctaatgaac aggcagcgcc tgaatcagtg gaagaaattc cagttaaaca
     1201 taatgaggac acgcaatcac tggaaaatgt ctcatctgta gaaacgaaat accaggaact
     1261 gagggaggaa ctcaataaag ccagggaaaa catcccccca aaaaatccag tcgatgccga
     1321 caaattactg gctgcctcgc gtggagaatt cgttgaaggc atcagtaacc ctgctgatcc
     1381 gaagtgggtg aaggggatcc agactcgcga cactgaggac cagaatcagt ccaaagtgga
     1441 acaaattgcc ccagaagcgg gacaaaacag cccggatacg caacaaaacg ggccagaaga
     1501 gcaacagcca gggccagtag cgcaaccgga gctggaaaaa aactgccgcg tctgtggtca
     1561 gactggcggg ggtaactgcc ctgactgtag tgcggtaatg ggcgatagca cttacacaga
     1621 aacttttgga gaaaatgacg ccgctgatgg agaagactca gcacaaactg aggagaagat
     1681 cattcaggaa aactctgttg atgccgctca ggagggcgaa accgttgttc agaacgagcc
     1741 aggcagtgat acgtccggcg atgacgccaa ttctgagcca gtaactctcg actggaaaag
     1801 acagctcgtg attgccgccg tctatggttt gtgcgccaac cccgcatgta tagccacagc
     1861 gccagcaatc cctgatatcg ccatcatgat tgccaacagg cttgaaaatt tcggaggtga
     1921 taaatcatga atgcctggct tatccccgat cgcattgaag agcagtcatg ggcacgacac
     1981 taccagcaaa ttgcccgtga agaaactgaa gctgagctgg cagacgacct agaaaaaggt
     2041 ctgccccaac acctgtttga atcgctatgc atcgataatc tgcaacgtca cggggccagc
     2101 aaaaaagcta tttcccgtgc atttgatgac gatgtcgatt ttcaggaacg catggcagaa
     2161 cacatccgct acatggctga aaccatcgcc cgtcaccaaa ttaatattga ttcagaggta
     2221 taaaacggat gagtacagca ctcgcaacgc tggcagggaa gctggctgaa cgtgtcggca
     2281 tggattctgt cgacccacag gaactaatca ccactcttcg ccagacagca tttaaaggtg
     2341 atgccagcga tgcgcaattt atcgcattgt tgatcgtcgc caaccagtac ggccttaatc
     2401 cctggacgaa agaaatttac gccttccctg acaagcagaa cggcatcgtt ccggtggttg
     2461 gcgttgatgg ctggtcccgc attatcaatg aaaaccagca gtttgatggc atggactttg
     2521 agcaggacaa tgagtcctgt acatgccgga tttaccgcaa agatcgcaat cacccgatct
     2581 gcgttaccga gtggatggat gaatgtcgcc gcgcaccatt caaaacccgc gaaggcagag
     2641 aaatcaccgg accgtggcag tcgcatccca aacggatgtt acggcacaaa gccatgattc
     2701 agtgtgctcg cctggccttc ggatttgctg gcatctatga caaggatgaa gccgagcgta
     2761 ttgtcgaaaa tactgcatat actacagaac gtcagccgga acgcgacatc accccggtta
     2821 acgaagaaac catgtcggaa attaacgccc ttcttacttc catggaaaaa acgtgggatg
     2881 acgacctgtt gccgctctgt tcccagattt ttcgccgcaa catttacaca tcttcagaac
     2941 taacacaggc tgaagctgtg aaggttcttg gattcctgaa acagaaagtc acagagcaga
     3001 aggtagcagc atgacaccgg acattatcct gcaacgtaca gggatcgacg tgagagctgt
     3061 cgaacagggg gatgatgcgt ggcacaaatt acggctcggc gtcatcaccg cttcagaagt
     3121 tcacaacgtg atagcaaaac cccgctcagg aaagaaatgg cctgacatga aaatgtccta
     3181 cttccacacc ctgctggctg aggtttgcac cggtgtggct ccggaagtta acgctaaagc
     3241 gctggcctgg ggaaaacagt acgagaacga cgccagagcc ctctttgagt tcacttccgg
     3301 cgtgaatgtt actgaatccc cgatcatcta tcgcgacgaa agtatgcgca ccgcctgctc
     3361 tcccgatggt ttatgcagtg acggcaacgg ccttgaactg aaatgcccgt ttacctcccg
     3421 ggatttcatg aaattccggc tcggtggttt cgaggccata aagtcggctt acatggccca
     3481 ggtgcagtac agcatgtggg tgacgcgaaa agatgcctgg tactttgcca actatgaccc
     3541 gcgtatgaag cgtgaaggcc tgcattatgt cgtggtcgaa cgggatgaaa aatacatgga
     3601 ggtttttgac gaaatggtac cggagttcat cgaaaaaatg gacgaggcac tggctgaaat
     3661 tggttttgta tttggggagc aatggcgata gccgtagcaa cgaggtgcaa atgatatgac
     3721 aattaaggaa tgctacctgt gttcgttctg atccagcgcg ggcagtcctt cgtcgacgcc
     3781 aacaactatc cggttcaagt ctgcaaggta acgctgacac aggtgatctt ccgaaggctg
     3841 gatgggaaaa ccagagccgc atcaattaac tcatttaatg cagaatttga gcgaatcgat
     3901 cacaacgaac tacatatgat taaagcggaa attgagaagg aaaagcatat tgccagcctt
     3961 cgaaaaatgc gccgcacatc aatcaactga caaccgcctt cgggcggttt tttcttacat
     4021 atgcggaggt aacttatgcg cgagttcgta aaccaacata gccatggcat tcaaccagtc
     4081 atcacacctg ttgtacagat aaatgcgaat gaatgggtaa cactggagct tttaatggct
     4141 gtaacaggcc tgagaaaagg aaccatatta cgcgccaggg acgctgcgtg gatgaacggc
     4201 agagaatata aacaaatcgc ccccgacgga acgccaaaga aaaacagcga atgtctctat
     4261 catcttccta ccatcaacac ctggattaaa aaccaaccct taccatctca ggatgtttaa
     4321 ttcttgtcca taagagtata acctgagcgt gctcttggac gcaggaggaa caatggcgaa
     4381 ttcagcctat ccagccggcg ttgaaaatca cggaggaaaa ctccgaataa cgtttaagta
     4441 caggggtaaa cgagtgcgcg aaaatcttcg cgtgcccgat acaccgaaaa acagaaagat
     4501 cgctggtgag ttaagggctt cggtctgctt tgcaatcaga acaggaacgt ttgattatgc
     4561 cgagcgattc cctgactcac ctaacctgaa gctatttggc ctggtaaaaa aagatatcac
     4621 cgtcggtgaa ctggcacaga aatggcttac tctgaaagca atggaaatca gtagtaacgc
     4681 cttaaatcgt tatcaatcag tgatgaaaaa tatgctaccg aggcttggcg tgggtagact
     4741 ggcatcctcg attacaaaag aggatttgct gttcatcagg aaagatttac tgactgggga
     4801 aaaggaaagc aggaaaaaca cttccaccaa caaaggaaga acagtcccca cggtaaacta
     4861 ttacatgaca acaactgccg ggatgttcag ctttgccgct gaaaacgggt atctggagaa
     4921 aaacccgttt aattcaataa caccactgag gaaatcaaaa cctgtgccag atccactgac
     4981 cagagaggag tttggtcgcc tcattgatgc ctgtcatcat caacagacca aaaacctctg
     5041 gacagtggct gtttttacgg ggatgcgaca tggtgaaatt gccgcacttg catgggagga
     5101 tatcgacctg aaagttggca cgataacagt gcgaagaaat tttacaaaaa taggtgattt
     5161 tacgctacca aagactgatg ctggcaccga ccgggttata catcttctgg caccagccat
     5221 tgaagcactt aaaaaccagg cgatgcttac tcgtcttggc aggcagcatc agatcaccgt
     5281 taaattacgc gagtacggaa gaacaatttt gcacgagtgc acttttgttt tctgtccgca
     5341 aatcgttcgc aagaatcaca aggcgggtat taactatgcg gtaagctcca tcggggcaac
     5401 atgggactca gcaatgaaaa gagcgggtat aagagcccgg aaagcatatc agtcacgcca
     5461 tacttatgcg tgctgggctt tatctgccgg agcaaacccg acatttattg catcacagat
     5521 ggggcactcc agcgccagca tggtctacaa tgtttatggt gcatggatgc ctgagtgcag
     5581 cgtgactcag gttgctatgc tgaataatgt ccttaatgcc cgtgccccag acgtgcccca
     5641 aagtgaccag gaggatgaaa taaaattata tttttcaaaa tgataaagca tatccttaga
     5701 catgttgaaa cgatggcacg cgcagttgct gagggtgcaa gcaaagtgga tggcgcagaa
     5761 gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa agcaggcggt
     5821 aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg ccgattacga cgccattatt
     5881 tttggtacac ctacccgctt tggtaacatg tccggtcaaa tgcgtacctt cctcgaccag
     5941 acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt ctttagttcc
     6001 accggtactg gcggcggtca ggaacaaacc atcacatcca cctggacgac ccttgcgcat
     6061 cacggcatgg tgattgtccc cattggctac gcagcgcagg aattatttga cgtttcacag
     6121 gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg ttcacgccag
     6181 cccagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc aggtctggca
     6241 gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa gaagcgaaag
     6301 ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata gccgaggcca
     6361 ttttcgaagt cgccgggtac gacgaaaaaa tggcggaaaa gatttgggaa gaaggtagcg
     6421 atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt tggggcgaac
     6481 agaccatcga acgtaaaaac gtttaaccag cgactccccc gcttcacgcg ggggagtttt
     6541 ctgttatttc accgcttcat tcaatacgct atcaaactta tccattgggc aaaaaccgtt
     6601 ggcatctatc gggcaaccgc ttaattccag cgtcacgcgc tgcgcaggtg cctgcagggt
     6661 taacgcatcg gcattacgta actgttcccc actctggtac acatattcaa ttttcatcag
     6721 gtcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc caatcggcgt
     6781 gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta acagagaggc
     6841 aatgttggag tcgtgcccaa ctaataccgt gattttcggt gcactggtgc gatcggtgac
     6901 cagggctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca cttccggtga
     6961 ggtaaacagg ctgtcctggt aaccgttttt cagcttcgac aacaccttcc actgctggtc
     7021 agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtagt attgcaaagt
     7081 aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacgcccg gttcttgttg
     7141 atacttcgcg ctaaaggtat ttttgccatc tacaagcgaa cattgttgct tctctttaca
     7201 cgcaggagaa tctttatagt taacgatttg ttccagtagc ttgtagctgt cggtaagctg
     7261 gagtttgctg agctcgttct ccattaccgc caccgcctgt tcactgaatg cggcggaatc
     7321 atcggtgatc actgggttaa aggtggggtc catagtgccc attttttcct ggtgatgaac
     7381 aggaatatca cacccaggga atgcgccagt aataaagaac tgcgcggtcg cgacggtgcg
     7441 ttgcagacta ttggcatagg cataaacggt gtccggtggc gggcattccc ctgatttcac
     7501 catcccctgt tctgccagcc attcacgcat ataatggccc atatacactt cgagcacgcc
     7561 ccctttagtg gtgagctgcc caccggggac gtcccattca ggccatttat tcggcgtcga
     7621 ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc ggctcatcat
     7681 gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcat ttgaagcgag
     7741 taaaactatc cctgctacag ttgcggcgat tagcgttttg ttcattcctc acacctcttt
     7801 tgttatcaat aattaaagaa tgtgacagaa atatgtcatt tttccgggaa cagattcgca
     7861 aaatggctcg ttgaacgcct gtttttctat attccccttt aaaaacaggc aagttttgac
     7921 gcaaactatg cctgattagc cccggcctcg tttacgccgc taatgattac atcagtagct
     7981 ataaagaaaa tcagcggcgt taccattacc aataaaaaaa gaatgtgaga ctaatagaaa
     8041 tg
//
LOCUS       DAZGJT010000158         8010 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZGJT010000158 DAZGJT010000000
VERSION     DAZGJT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8010
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.161"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..903
                     /gene="yjeM"
                     /locus_tag="ACHKFK_004404"
     CDS             <1..903
                     /gene="yjeM"
                     /locus_tag="ACHKFK_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO9054608.1"
                     /translation="PGYQSGLAMLSFVVFAIFAYGGIEAVGGLVDKTENPEKNFAKGI
                     VFAAIVISIGYSLAIFLWGVSTNWQQVLSNGSVNLGNITYVLMKSLGVTLGNALHLSP
                     EASLSLGVWFARITGLSMFLAYTGAFFTLCYSPLKAIIQGTPKALWPEPMTRLNAMGM
                     PSIAMWMQCGLVTVFILLVSFGGGTASAFFNKLTLMANVSMTLPYLFLALAFPFFKAR
                     QDLDRPFVIFKTHMSAMIATVVVVLVVTFANVFTIIQPVVEAGDWDSTLWMIGGPVFF
                     SLLAMAIYQNYCSRMANKPELALD"
     gene            966..1280
                     /gene="yjeN"
                     /locus_tag="ACHKFK_004405"
     CDS             966..1280
                     /gene="yjeN"
                     /locus_tag="ACHKFK_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFO9054609.1"
                     /translation="MDDASRDPVITEDEIRELQFSAGDVAEIEQTVLSFVDTRHTRKV
                     AMVVGNTINTLKERDGPRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPI
                     IS"
     gene            1277..1591
                     /locus_tag="ACHKFK_004406"
     CDS             1277..1591
                     /locus_tag="ACHKFK_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFO9054610.1"
                     /translation="MSARMFILCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEDDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(1622..4945)
                     /gene="mscM"
                     /locus_tag="ACHKFK_004407"
     CDS             complement(1622..4945)
                     /gene="mscM"
                     /locus_tag="ACHKFK_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO9054611.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     NDALNQEILQISSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTEQLAESSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGSDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(4967..5935)
                     /gene="asd"
                     /locus_tag="ACHKFK_004408"
     CDS             complement(4967..5935)
                     /gene="asd"
                     /locus_tag="ACHKFK_004408"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO9054612.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWPTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDDSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(6032..7084)
                     /gene="rsgA"
                     /locus_tag="ACHKFK_004409"
     CDS             complement(6032..7084)
                     /gene="rsgA"
                     /locus_tag="ACHKFK_004409"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO9054613.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMEFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7179..7724
                     /gene="orn"
                     /locus_tag="ACHKFK_004410"
     CDS             7179..7724
                     /gene="orn"
                     /locus_tag="ACHKFK_004410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO9054614.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSNEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7935..8010
                     /locus_tag="ACHKFK_004411"
     tRNA            7935..8010
                     /locus_tag="ACHKFK_004411"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7968..7970,aa:Gly,seq:gcc)
BASE COUNT         1813 a         2091 c         2157 g         1949 t
ORIGIN      
        1 ccaggttatc agtccggtct ggcaatgtta tcgtttgtgg tatttgctat ttttgcctat
       61 ggcggaattg aagcggttgg tggtctggtt gataaaacgg aaaatccaga aaagaacttt
      121 gccaaaggta ttgtttttgc cgctattgtt atttcaatcg gttattcgct ggcaatattt
      181 ttatggggcg tcagcacaaa ctggcagcag gtattaagta atggttccgt taacctcggc
      241 aatattacct atgtgctgat gaagagcctc ggggtgacgc tgggtaacgc actgcatttg
      301 tcacctgaag cgtcattgtc gctgggcgta tggtttgcgc gtattaccgg actgtcgatg
      361 ttcctcgctt ataccggtgc gttctttaca ctttgctatt caccgctgaa agccatcatc
      421 caggggacgc cgaaagcatt gtggccggaa ccgatgacgc gcctgaatgc gatggggatg
      481 ccgtctatcg ccatgtggat gcagtgtggg ttggttactg tcttcatcct gctggtttcg
      541 tttggtggcg gtaccgcatc ggcgttcttt aacaagctga cgctgatggc gaacgtgtct
      601 atgacgcttc cttacctgtt cctcgcgctg gctttcccat tttttaaagc acgtcaggat
      661 ctcgacagac cgtttgtgat tttcaaaacg catatgtcgg caatgattgc aacagtggtt
      721 gtcgtactgg tggtgacatt tgcgaacgtc ttcaccatca ttcaacctgt ggttgaagct
      781 ggagactggg acagcacatt gtggatgatt ggcggccctg tcttcttctc gctgttagcg
      841 atggcgattt accagaacta ttgcagtcgc atggcgaaca aacctgagtt agctctcgac
      901 tgatcatatg aaatcctgca actcatcgtc tctccggcga tgagttgtct ggaaaggaag
      961 acgttatgga cgacgctagt cgggatccag tgataaccga agatgaaatc agggagctgc
     1021 aattttctgc cggggatgtg gcggaaatag agcaaacggt cctctctttt gtcgataccc
     1081 gccacacccg caaagtagcg atggtggtgg gtaacactat taataccctc aaagagcgcg
     1141 atggtccgcg gtggggaaac ctgccggata tctattgcgc ttatcttatt agatgtctgg
     1201 tttttcgcgg agaattagtg ggatacggcg atttgtttcg tatgcgctat agcgaaatta
     1261 agcgaccgat tatctcatga gtgcgcgcat gtttatcttg tgctgcatct ggtttattgt
     1321 ggcgttcctc tggataacca tcacttccgc gctggacaaa gagtggatga ttgatggacg
     1381 cgggattaat aacgtctgcg atgtgctgat gtatctcgaa gatgacgata cccgtgatgt
     1441 cggtgtgata atgacgttac cgctcttttt cccttttctc tggttcgctt tgtggcgaaa
     1501 aaaacgcggc tggtttatgt acgccaccgc gctggctatt ttcggctatt ggctgtggca
     1561 gttttttctg cgctatcagt tttgcttata agccggatgg gttcatccgg ccacaaagac
     1621 cttacaaact tcccgcctga cgacctttgc ccgcagaagt taatgttctc cccgtttgtt
     1681 taccattcag gctttccaga cgcatctgga agggcgggaa tggcatatcg ataccgtggg
     1741 cgtggaagcc cgccagaatt agttggtgga tctcatggcg tagcggcata cggtgaccca
     1801 tctcagcggc gtaaatacgc agctcgaaaa tttgaatccc ctgttgcaga tccaccagga
     1861 agacttccgg cgccgggttg tcgatcacca gcgagcagcg acgcgctgcg gtgagcagga
     1921 tttccgtcac ttcttcactg ttagcatcgg caggggccgg aatcgtcaat accacgcgcg
     1981 tgaccgagtc agagagcgac cagttgataa actgctcggt aataaacgcc ttgttcggca
     2041 cgattatctc tttacggtcc cagtcgctga tggtggtagc gcgggtgtta atttttgtca
     2101 cgctaccggt gagatcgcgg atcgttaccg tatcgccaat acgaatcggt ttttcgaaca
     2161 ggataatcag gccagagata aagttggcga aaatctcctg taaaccaaag cccagaccaa
     2221 caccgagcgc ggcgaccagc cactgcaatt tcgaccactc aataccaatc atcgagaagc
     2281 cgaccagccc gccaatcagc atcagcagat atttggtgat ggtggtgatg gcgtagcccg
     2341 tacccggcgt taaatccagg tgctgcagaa tcgccagttc cagcagcgcg ggtaggttgc
     2401 gcaccagctg cgtggtgatg ataaacacca gaatggcaat cagtaccgca ccgagggtaa
     2461 tcggctccag gctttctacg ccctgtaccg tggaagtgac atcccacagc gaaatatttt
     2521 cgaggaagcc gaaagcggaa tggatttctg accacagcac aatgaccgaa agcagggcga
     2581 tcaacatcaa aattgagcgc accagccgca aggattgcgc actgatggca tcgagatcga
     2641 cttcgctttc atcgacttca attgctcctt ccgggctact gtgatgatgc gcttcctctt
     2701 cgccacgcgc acgctgcgct aacatctctg cccggcgatg cttcgcccgg tcaaacgcca
     2761 gcctgcggcg ctggatcagc atccagcggc ggataacgtg ataaaccacc agtagcagga
     2821 accagatggc aaccgaggtt tcaagcctcg ctaacaatgc ctgcgccgtt gccagataac
     2881 ccaccgccga cgccagaatg gcaaccagcg gtgcgccaat catcatgttc cacagcatat
     2941 ggttggtaat gttgtcgccg ctgccttctt tgttgagata cagcgggatc ccggcctttt
     3001 tcaggctgag ggtgaccacc gccagcgcac cgcaaatgag gataaagcaa agccgtccca
     3061 gcgaaccgga aaattcgcgg tcgtcgaggt tatcgaacat catcagcgcc ataatcagcg
     3121 gcacaataag cccgatgctc atcaggtagt aacgcatccc acgggaaaca cgttcgcgcg
     3181 gccagccaaa atgagcgata aacaagccgt tcgggcgagc aaaggtggcg caaatcatca
     3241 ctacccacag cagcggcacg gtggctgtta cgccatcacc aatcgcgacc gccagcggat
     3301 aaggccacgc ctcgcgcaag ccgtaaccca gcgtcatcca cagcacaggc agcggtgacg
     3361 cgacgagaat cgaccagaaa agagtgcgta acgtcagcca gaagtgatcc tgagtcactt
     3421 tgccgacttt cgccgccgaa cgttcaagaa aacgggtgaa atagcggcgc gagtaaatac
     3481 tgcaaccgac cagaatcaac gcgccaaaca gcggcaaaat cgtctcttta ctggtcagca
     3541 tcatcacact ggctttgccc aactgactga aggtgtccag cgaaatgaga cgacgcagat
     3601 cctgggcgat ttccagcggc caggcgatgg tcatcgggcg cacgtcagag gtccagaaca
     3661 gatagcggtg cgtcgcttcg ttcacctctt tcagtgcatc ctccagttgc ccgttggaga
     3721 ctttcagctt ggtcagctcc agcagtagcg tgtcgctacc ctgcaacaat gagttcagca
     3781 actcacgctg agtgcgtagc tgtgcttcca gaatacggtt ttgctcagcg gtcagcggct
     3841 gaccgtcggc ctgatggatt tgccgtagca gcggctgttt attaagcaga tcctcataac
     3901 gtaaccgttg cacacgtaac tgcgccattt cggtatcaag ctgttgtggt tttggcattt
     3961 ccggtagccg tgccacctgc gcccgcaacg cttcgccgag caggttagat gatcccagcc
     4021 attgcgactg ttcacgcagc gtattcaacg cctgccggac ctgtaacgtc tggctggcag
     4081 cctgacgctg ttgcgaggca acgagatcca tccgctgcgc ctgttgattc agcgccgccg
     4141 atagttcgcg gttaatttta aactgggcga cgatatcttt tggcaaatcg gcgctacttt
     4201 ccgccagttg ctcggtactt tccagcgccc gttccgcttc cagttggcgt tgactattca
     4261 actggttgcg tagtgcttgc aaatatgcat ccagctgctg gctctctttt tccgccagct
     4321 cagagcgcaa gcgcgctaat tcctggcggt tattggcaga tagctgcgcc agctccagtt
     4381 catcaacgag cgctttcaga cgtgcggagt cagactgcaa cgcgaaattt tgtgcctgat
     4441 tgagcggggt attgccggta agtgtcccca ggcggcgctc gatttcattc aactgacggc
     4501 gggcatcggt ttgctgttgc ggcagttgat tcagcgaatc ggcaatctcg cgggcgcgct
     4561 cctgctcttg ctgggcctga cggcttttat ccagcaactg actgctgatc tggagaattt
     4621 cctgattcag cgcatcgtta gacattcccg gcgacacgct gcgtggctcg tcacgcatgt
     4681 tgtttaactg cgcgcgcaga gtggcggaaa gtttcggata attatcaatg acttcttgat
     4741 attgcttgat gcgctcaagg gaaccttttc gttcctcaag cgcatttaag gcagactgga
     4801 gcgcctctac gacttccggc tgcgcaggtt tcgccgcttt tgcctgctcc agttcctggg
     4861 tgatttgttt gctatcgggg gccgtcgcgg cgtacgcccc ccaactgagg caccaggcca
     4921 tcagaaaagt gataatcagg cgcacgtcag cgtttccttt gatggattag acctggtctt
     4981 ttttgtcgtc aaccaatggg ctggcgtcgt gttctgcctc gatttcttca gcaggaagcg
     5041 gggctggttc agcgtctggc gtaacaaagg tttcggtaga tactgccagc ggctggccaa
     5101 ttttcgtgac agacaggctt tccagttgct caaccaggtt cactttacct ggtgcaaaca
     5161 ggttgataac ggtagagcca agtttaaagc gacccatttc ctggcctttc agcaatgcca
     5221 cagaatcgtc gttttccccg gcaggccagg tccagcgctt gatgatgcct tcgcgcggcg
     5281 gcgtaatggt gcccgcccag accgtctcaa tgctgcctac aatcgtcgca ccgaccagaa
     5341 tctgcgccat cgggccaaat tcggtatcga acaggcagat cacgcgttcg ttacgggcaa
     5401 acagattcgg cacgttctga gcagtgagat ggttaacgga gaagagatcg cccggcacgt
     5461 agatcatttc acgcagaata ccgttgcacg gcatgtgtac gcggtggtag tcacgtgggg
     5521 agaggtaagt ggtcacaaac gtaccgttac ggaagagatc cgccatcaga tagttgcctg
     5581 ccagcagggc ttcgaggctg tagttgtggc ctttggcttg caggatttta tcttcttcga
     5641 ttttacccaa ctggctgata acgccatcgg caggcataac cagtacattc ggatcggtat
     5701 cgattgggcg tacttcgtca cgcagcggac ggacaaagaa ttcgttaaag gtgcggtagc
     5761 tggcggtgtc cggcttttgc gcctctttca tgtcgacctt gtagtattta acgaacagat
     5821 cgataaccag ttttgtcggc catcctgccc gcttgcttgc gccccaaccc gccaggcgag
     5881 taagccatag tttcggcaga atgtactgta gcgaaagttt aaatgaattt aacaaggtag
     5941 cctccaggcc attgttttgt cgttcctgat ccggcctaca tgccggatcc tgaaaaaaag
     6001 gggacgattc taacgacggt tagcttaatt gtcagtcatc cgtatcagaa aagtttttac
     6061 gcgtttttac ctgcgccatg ctttccagaa tacggtgata gttttcgaaa cgggtttccg
     6121 cgattttccc ttcctcaacc gcttcccgga tagcgcagcc cggatcggta tcgtgtttgc
     6181 aatcgcgata tttacacaga cctaaatagt catggaattc gacaaagccc tgagtgattt
     6241 gttccggctc caggtgccag agaccaaact cacgcacacc tggggaatca atcacatcac
     6301 cgccgtgcgg gaagtgatac agtcgtgcgg cggtcgtggt gtgctgacca agccccgagt
     6361 tgtcagagac atcgttggtc aggatctctt tttgcagccc cagcaacgcg ttcagcaggc
     6421 tggatttgcc aacgccagac tgcccggcaa aaatgctgat gcgcccggtc aacgcctctt
     6481 ccagcggttt cagcccatcc tgagtatgac tggaaaccat caatacgcga taaccgatat
     6541 tgcggtagat atccatctgc tcgttgacga actccatgcc ttcgtcgtcc agcaggtcta
     6601 tcttgttgag cacaataatc ggctcaatct gcaaggtttc gcaggccacc aggtaacggt
     6661 cgataatatt gagcgacagc tccggcaaaa tggcggagac aatgacaatc tggtcgatgt
     6721 tggcagcaat aggtttcaca ccgtcgtaaa aatccgggcg cgtcaacact gaggtgcgct
     6781 catgcaccgc ttccacgatc cctttgacat ttacgccttc cgccgctggt ttacccggac
     6841 gccagactac gcggtcgccg gtcaccaacg aacggatggt acggcgaata ttgcagcggt
     6901 gaacgtcgcc atcggcggat tccacatcag cgtgcatacc aaagcggctg atgacgatac
     6961 cttcatcagg ctcgccaaac agattgtcgt cgtagtcggg cttctcctta gacgttttaa
     7021 gacgacgctg gtgattggcg ttcacgcggc gctgctggcc tttggagagt ttatttttac
     7081 tcaatcgtac agactcctgg tcgcccctga tgggcaaaac atctatgata cacgcaattg
     7141 tggatcaata tagtcactgt gaatgggtgg aaaatagcat gagtgccaat gaaaacaacc
     7201 tgatttggat cgatcttgag atgaccggtc tggatcccga gcgcgatcgc attattgaga
     7261 ttgccacgct ggtgaccgat gccaacctga atattctggc agaagggccg accattgcag
     7321 tacatcagtc caatgaacag ctggcgctga tggatgactg gaacgtgcgc acccataccg
     7381 ccagcgggtt ggtagagcgt gtgaaagcga gcacgatggg cgatcgtgaa gctgaactgg
     7441 caacgctcga attcttaaaa cagtgggtgc ctgcgggaaa atcgccgatt tgtggtaaca
     7501 gcatcggtca ggaccgtcgc ttcctgttta aatacatgcc ggagctggaa gcctacttcc
     7561 actaccgcta tctcgatgtt agcaccctga aagagctggc gcgccgctgg aagccggaaa
     7621 ttcttgacgg ctttaccaag caggggacgc atcaggcgat ggatgatatc cgtgaatcgg
     7681 tggcggagtt ggcttactac cgcgagcatt ttatcaagct gtaaaatttt tcctggtcac
     7741 gtaagcgccg gaatccactt gcggcggcgt gattttgacg ctaaattgaa cgtcttgctg
     7801 ataatttcag cacttgagat aaaaacgcaa aaaaaatttt ttaggggggt tgcagaggga
     7861 aagatttctc gtataatgcg cctcccgtaa cgacgcagaa atgcgaaaat tacgaaagca
     7921 aaattaagta gtacgcggga atagctcagt tggtagagca cgaccttgcc aaggtcgggg
     7981 tcgcgagttc gagtctcgtt tcccgctcca
//
LOCUS       DAZGJT010000159         7980 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZGJT010000159 DAZGJT010000000
VERSION     DAZGJT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.162"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(251..1615)
                     /gene="tdcG"
                     /locus_tag="ACHKFK_004412"
     CDS             complement(251..1615)
                     /gene="tdcG"
                     /locus_tag="ACHKFK_004412"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO9054615.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEELLSKTYYSVGGGFIVEEEHFGLS
                     YDVETPVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1687..2076)
                     /locus_tag="ACHKFK_004413"
     CDS             complement(1687..2076)
                     /locus_tag="ACHKFK_004413"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO9054616.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2090..4384)
                     /gene="tdcE"
                     /locus_tag="ACHKFK_004414"
     CDS             complement(2090..4384)
                     /gene="tdcE"
                     /locus_tag="ACHKFK_004414"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO9054617.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPLDFDTNIATTITAHDAGYIDQPLEKIV
                     GLQTDAPLKRALHPFGGINMIRSSFHAYGREMDSEFEYIFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRRALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEALPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGMDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPIRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4418..5626)
                     /gene="tdcD"
                     /locus_tag="ACHKFK_004415"
     CDS             complement(4418..5626)
                     /gene="tdcD"
                     /locus_tag="ACHKFK_004415"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO9054618.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLNASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDNVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLHRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5652..6983)
                     /gene="tdcC"
                     /locus_tag="ACHKFK_004416"
     CDS             complement(5652..6983)
                     /gene="tdcC"
                     /locus_tag="ACHKFK_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO9054619.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7005..>7980)
                     /gene="tdcB"
                     /locus_tag="ACHKFK_004417"
     CDS             complement(7005..>7980)
                     /gene="tdcB"
                     /locus_tag="ACHKFK_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO9054620.1"
                     /translation="DLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEIFLKFE
                     NMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGKVVMPK
                     GAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIAGQGTI
                     GLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAASFHSG
                     EITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQRNKVVT
                     EGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         2047 a         2100 c         1903 g         1930 t
ORIGIN      
        1 gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt ttgcaggcga
       61 gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca atcaagactt
      121 tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga atattcattc
      181 tctatttgcg gcagatcaca aaaaaaaagg tgcacatttg tgcacccaag gatgaaagct
      241 gacagcaatg tcagccgcag accactttaa tagccagtcc tccgcgtgat gtttcgcggt
      301 atttatcgtt catatctttg ccggtttcat acatcgtctc gatcacttta tcgagtgaaa
      361 cacgcggtgc cgaggtgcgg cgcatcgcca tccgcgcggc gtttactgct ttcacggcat
      421 taatagcatt acgttcaatg cacgggattt gtacctgtcc ggcaaccgga tcgcaggtca
      481 gcccaaggtt atgctccatc gcgatttccg ccgcattgca tacctgcgcc ggactgccgc
      541 ccagtagttc agttaaccct gccgccgcca ttgaacaggc cacgccaatc tccccctgac
      601 agccgacttc cgcgccagag atggaggcgt tcattttata cagcgcgcca attgcccccg
      661 ctgccagaaa atagcgggca attgaccgct cgtttaccgg acgacggaac ttatcgtaat
      721 aagccagcac tgccggaata atgccgcacg caccgttagt cggtgccgtt accacgcgcc
      781 cgccagctgc gttttcttca ctaaccgcca gcgcgtacat gttgatccag tcgatgacat
      841 tcatcggatc gttagagatg ttatcgctgg aaaccagttg acgacgcagc gctacggcac
      901 ggcgcggcac attgagcgga ccaggcagca cgccttcagt attcattcca cgttcaatac
      961 cgtcatgcat cacttgccag atacgggcaa accctgcgtc aatttccgct ttactgcgca
     1021 gcgccagctc gttgtgcatc atcaggccag atatagacag gccgttgtaa tcacacattt
     1081 tcagcagttc acctgctgag tggaaatcgt aaggtacggg tgtttcgaca tcatacgaca
     1141 ggccgaagtg ttcttcttcg acaataaacc cgccgccgac agagtaatag gttttgctta
     1201 atagctcttc ctgtcccttc caggcagtga tccgcattcc gttctcatgg cgaggcaaca
     1261 tttcgggatg gaagataatg ttctttgcta caggaaaatc aacaatatgc gcgccagaag
     1321 ccaccggcaa ccgcccgctg cgcgtcacta actctataaa tgcagggatc tcatcaatga
     1381 caacatcctg cggactgttt cctgccagtc ccatgatgat ggcgacatcc gtggcatggc
     1441 ctttgcccgt cagtgacaac gacccgtaca gatcgaccac aatatggctc gtcgcggtta
     1501 ataagccgct actttccagc cgatcaataa aactttttcc ggcattcatt ggccccacag
     1561 tatgcgaact ggagggacca atcccaattt tgaaaatatc gaatgcacta atcatatcca
     1621 caccctcgga ttgccgttca gtaaagtgga gcggaacgac cttgcgaccg tcccgctcac
     1681 gaggccttac gcactacgta ctgcgatggc ttcaatttcc aacttcacat ctttcggcaa
     1741 acgcgcgacc tgcacacagc tccgggtcgg gtaggtcgcc tgatgttcat cgaagaactg
     1801 cttatagact tcattgatgg tggcaaaatc attcagatcg gtgatgaaca cggtcatctt
     1861 gatgatatcg cccacactca gcccggcagc aaccacgatc gctttgacgt tttcgaggct
     1921 taaacgcgcc tgatcttgca catcagccgg gatctcaccg gtctgtgggc aaaccggaat
     1981 ttgcccggag gtgaagacca tgctgcctaa atcaacgccc tgaacataag ggccgattgc
     2041 gcctggggca cgttgcgttt cgataatctt tttcatacat cctccggcgt cagagcgcct
     2101 gggtaaaggt acgtgaaata acatcctgtt gctgttcacg ggtcagtgcg ttgaagcgca
     2161 cggcgtagcc agagacacgg attgtcaggt taggatattt ttccgggtgc tcgatggcat
     2221 ccagcagcat ttcccgattc attacgttga cgttgaggtg ttgaccgcct tcgacatccg
     2281 cttcgtggtg gaaataccca tccagcaggc cgacaaggtt cgttttacgt atcggatctt
     2341 ctttgcccag cgccgcaggg acgatggaga aggtgtacga gatcccatct ttggcgtagg
     2401 tgaatggcag tttcgccacc gacgtcaatg aggctacggc gcctttgcga tcacgaccgt
     2461 gcatcgggtt agcgcccggc gcgaacggtg ttccggcgcg gcgaccgtcc ggcgtgttcc
     2521 cggttttctg accgtacacc acgttagagg tgatagtcag aatcgattgg gtcggcacag
     2581 cattgcgata ggttggcaaa gctttaattt tcttcataaa gcgttcaacc agatcgcagg
     2641 caatgctgtc tacgcgctcg tcgttgttgc catactgcgg atattcgccg tcaatctcga
     2701 aatccaccgc caggccgttt tcatcacgga ttggtttcac acgagcatat ttgatggcag
     2761 acagggagtc cgttgccacc gacaggcccg cgatgccgca tgccatggtg cgatagacat
     2821 cacgatcatg tagcgccatc agcgaagctt cgtagctgta cttgtcgtgc atgtagtgaa
     2881 tgatattcag cgcgctgatg tactgtaccg ccagccagtc catgaagtgg tcgaggctat
     2941 ccatcacttt gtcgtagtcc agcacgtcat ccatcaacgg agccgttttc gggccaacct
     3001 gaatcttcag cttctcgtcc attccgccgt tgattgcgta cagtagcgtt ttcgccaggt
     3061 tagcgcgcgc accgaagaac tgcatctgct taccaatcac cattgggctg acgcaacagg
     3121 caatcgcgta atcgtcgcta ttgaagtcag tacgcatcag atcgtcgttt tcatactgca
     3181 aagaggatgt gacgatagac acctgcgcgg catatttttt gaaggcaatc ggcaaggctt
     3241 ccgaccacag aatagtcagg ttaggttccg gtgcaggccc catagtgtgc agggtgtgta
     3301 aatagcggaa ggagtttttg gtcaccaggg ttcgaccgtc cagccccatc ccgccgatca
     3361 cttccgtcgc ccagatcggg tcgccggaga acagcgaatc aaattccggc gtacgcaaga
     3421 agcgcaccat acggatcttc atgatgaagt ggtcgatcag ttcctgtgcc tgctgctcat
     3481 tgagtacgcc tgctttaaag tcgcgctcaa tgtagatatc gaggaacgat gccgtgcgcc
     3541 ccagcgacat cgcgccgcca ttttgcgatt tcactgccgc caggtaagcg aagtagagcc
     3601 actgcaccgc ttcctgtgca ttctgcgccg gacgggagat gtcaaaaccg tatttcgccg
     3661 ccatttcttg aatctgcaac agcgcacgac gatgttccgc cagctcctca cgcagacgga
     3721 tcgtagcttc gagatcttcg cctttttcca gacgggactg gagatcggca aactgcagtt
     3781 cgcgttcacg taccagataa ctgatgccgt aaagcgccac gcggcggtag tcaccgatga
     3841 tacgcccacg accataacca tctggtaaac cggtcagtac accagattta cggcagcgca
     3901 gcatatccgg tgagtaaaca tcaaatacgc cctggttatg ggttttacgc agatcggtaa
     3961 agatgtattc aaattcgcta tccatttcac gaccatacgc gtggaatgaa cttctaatca
     4021 tattaatgcc gccgaacgga tgcagcgcac gctttaatgg cgcatccgtt tgcagaccca
     4081 ctattttttc cagcggctgg tcaatatatc ctgcatcatg agcggtaatt gtggtggcaa
     4141 tattggtatc gaaatccaat ggcgcgtgag tcgcattttc gatacggatg ccttccatga
     4201 ctttttccca caattccgtg gtggcaggcg tcgcttcggc gagaaaagat tcatcgcctt
     4261 cataaggtgt atagttatgt tgaataaaat cgcggacatt aatttcgttt ttccagtccg
     4321 tacctttaaa gccaagccat gcgtcggcgt acagcttatc gctggtatca atatctacct
     4381 tcatgaaaaa taatctctct acaatacttc aactaaatta tgcaaattct gcgggcgcgt
     4441 tcactttgcc taaatgaatg gcatccaaag caatcatttt ttcttcgtta gtcggaatta
     4501 cggcgcaaat gacacgcgca ttttcactgg aaacaattcg ctctccaaaa gagttagagc
     4561 gattattcat ttctgtatca atttctacgc ctaatacagc taaatgttcc atgaccagac
     4621 gacgaattaa acttgaattc tctcctattc cgccggtgaa tataattcca tccaggcgat
     4681 gtaatgaagc agcgtgtccg gcaatatgac gggcaattcg gtgaacaaag gttttaattg
     4741 ccagttgcgc gcgttcgtga ccttcatgcc aggctttttc cagaacacgt aaatccgaag
     4801 aaaggccgga aatacctaat aatcccgact ctttattcac tacgcgttcc aggtccccca
     4861 ggctctggtt ggtttggctg gcgacccagg acatcgcccc gaagtcgaca tcaccactgc
     4921 gggtacccat catcaagcct tccagcggcg tcattcccat ggaagtgtca acactctgac
     4981 cgttgcgaac cgcgcagatt gacgcgccat tgccaagatg cgccacaacc aggccggagt
     5041 catcttccgc cagattcagc agagaatgcg cgcgctggga aacatagcgg tgcgacgtgc
     5101 cgtggaaacc ataacggcgt acacctaact cttcataata tttccacggc aggccgtata
     5161 aataagcttc cggagccatc gtctggtgga aactggtatc aaataccgcc acctgagtta
     5221 cgcccgggaa taattgctgc gccgattcaa taccacttaa attggcgtaa ttatgcaggg
     5281 gtgccagtgg agaaacgcga cggatattat caatgacttc atcggtaata atggcggact
     5341 cggtaaaaat actgccgccg tgagcgatgc ggtggccaat taaggccaca ttgtcattta
     5401 aattccgttt ttccagttca aatgcaattg ccttcaatgc accttcgtag ctgtggtgag
     5461 ccagcggtgc tggctctccc ccatttacgg ataagaatgc attttccgag ttaataccgt
     5521 cggcaatacc tgacattaat acttcacagt cgctggcatt gagtacggaa aacttaatcg
     5581 aagacgaacc acagttaata accaaaacaa ccggaaattc attcatctct tttctcatcc
     5641 tgagttacgg attaaaacag tttgtatacg atgttcagga tggtcagcag accaatcacg
     5701 gtaacaaaca cgttatccag acgaccacgg tatttcgcca gagacggcgc tttacggatg
     5761 gcatacatcg gcaacaggca cagcagtgat gcgataatcg gtgcgcccat ggcttcaatc
     5821 aggtcgagga tgttcgggtt ggcgtaggca acaacccagg tggagcccat aatgaaaatc
     5881 atgctgatag tattcagttt acccagcgac actttggttt tgtcaccttt ataaccgaac
     5941 ttcagaatca aaccattcag cccttccagc gtccccagat agtgaccgaa gaaagatttg
     6001 aaaatagcca cgagtgcgat gatggaagcg gcatattcca gtgtaatcgc aaacgttgtt
     6061 ttggtaccgg tcatggacgc aaagtggtta gccagataag aaagcactgg aatattctgc
     6121 gctttcgctt cagccatatt cgccggagac agcgtaaaca ggcagctaaa ggcaaagaac
     6181 atcaccactg caaccatcag catactggcg cgagagatga tttgtgaaca tttacgctcg
     6241 gtgaagtcgc gaccaaaatc tttctcgtac tcttcacgtt tagaaaccac gaaggaagag
     6301 acgattggcg agaagttaaa ggagaaaacc atgatggaaa tccccagcca gacggtgatc
     6361 aggataccgt catgaccagt taacgacagc gaaccgaggt caacctggtc gataactgca
     6421 gagttccagt aagggatcag cgaaagagaa atcagcacca ggctggcgat aaacggccat
     6481 accagatagc tcatcacttt aaccatcaga tccttaccaa accagatgac gaaagccatc
     6541 agcagcaaca ggaacagcgc cacaaagccg cgattcagcg gtgcaaagcc gagctggttt
     6601 tcccagaacg tcataaaggt attggtaata gtaacgccat aaatccacaa cagtgggcaa
     6661 atcgcgaaga agtacaggaa cgtgataacc acgccgccag ttttaccaaa atgctcttcc
     6721 accgtttccg taatgttgcc ggaagggtta gagccagaaa gacacagacg cgccagtgcc
     6781 cggtggcaat aaaacgcgat ggggtatgct aataccaaca tcagaagaat cgggatcagt
     6841 ccgccaaaac ctgcgcggat agggaagaac agcaccccgg cgccgattgc cgtaccaaac
     6901 aagcctaacg tccatgtggt atctgattta cgccaggacg attgttttgt ctggctggat
     6961 acaatgctat ctgaagtact catatcctat cctcaacgaa ttaattaagc gtcaacgaaa
     7021 ccggtgattt gagagacgcg agaaagatcg atattgccgc cggaaataat actgacggtt
     7081 tttctgtttt gaatatattg gtctaattta ccgcttaata atgcagcaca tgccagagcg
     7141 cctgcgcctt cggtgacgac tttattgcgc tgaattaagg caatcatact gtttctgatt
     7201 tcgtcttcgc tgaccagcac gatgtcatcg actaattcac gaacgatttc gtaagttaaa
     7261 ttacccgggc gggagacatc acaaccatcc gccagggtgc cggtagttcg gtgcgtagtt
     7321 atttctccgg agtggaaaga agccgccatg ccgtgaacgt tttcagactg tacgccaata
     7381 acacggatgg tcgggttgat agatttaatt gccaccgcaa tacctgcaat taaaccgccg
     7441 ccgccaattg gcacaatcac gttatcgaca tcatagagat cttccataat ttccagacca
     7501 atcgttccct ggccagcaat cactttcgga tcatcgtaag gtgggataaa aatacggcct
     7561 tccatttcga caatttcgct cactttagca atagtgtcgt tgaagttatc accatgcaaa
     7621 acgacttctg cggagtagtc gcacgttgcc gcgactttgg atttcggcgc accttttggc
     7681 atcaccactt taccgtcaat acccagcatc gcgcaggaga gcgaaacccc ttgcgcatgg
     7741 ttgcccgcag agcaggccac tacaccttta cgtttttccg catcagtcag tgaacttaat
     7801 ttattaaatg cgccacgaat tttaaatgaa ccggtacgct gcatgttttc gaatttgagg
     7861 aatatttcac ctttgcaacg ttcactaaaa tagttggagc gaggcatacc tgttttataa
     7921 attcgcccag ccagtcgttg ttttgcttca atgatgtcat caatagcaac cgggagatcg
//
LOCUS       DAZGJT010000160         7976 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZGJT010000160 DAZGJT010000000
VERSION     DAZGJT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7976
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.163"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..706
                     /gene="lhgO"
                     /locus_tag="ACHKFK_004418"
     CDS             <1..706
                     /gene="lhgO"
                     /locus_tag="ACHKFK_004418"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO9054621.1"
                     /translation="GEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPE
                     HNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSMTVGPNAVLAFKREDYRKRDFSFSDTL
                     EILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQ
                     AVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRT
                     LRAARSVDALHAAFNQ"
     gene            729..2177
                     /gene="gabD"
                     /locus_tag="ACHKFK_004419"
     CDS             729..2177
                     /gene="gabD"
                     /locus_tag="ACHKFK_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO9054622.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNFMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGVPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQNGVYDRFAEKLQQAVSKLHIGNGLEKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKAHERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIIGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            2191..3471
                     /gene="gabT"
                     /locus_tag="ACHKFK_004420"
     CDS             2191..3471
                     /gene="gabT"
                     /locus_tag="ACHKFK_004420"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO9054623.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAT
                     PAFMLRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANVLGQKLKDG
                     LLAIAKKHPEIGDVRGLGAMIAIELFEDSDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            3708..5108
                     /gene="gabP"
                     /locus_tag="ACHKFK_004421"
     CDS             3708..5108
                     /gene="gabP"
                     /locus_tag="ACHKFK_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO9054624.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYADVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVLSTGLLAIGIICTVPIMAR
                     WKKLIMWQKTPIHNTR"
     gene            5129..5791
                     /gene="csiR"
                     /locus_tag="ACHKFK_004422"
     CDS             5129..5791
                     /gene="csiR"
                     /locus_tag="ACHKFK_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO9054625.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVTGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHNQHQILTEAVLARDAARASELMRQHLLTPIPIIQQAMA
                     CN"
     gene            complement(5792..6241)
                     /gene="kbp"
                     /locus_tag="ACHKFK_004423"
     CDS             complement(5792..6241)
                     /gene="kbp"
                     /locus_tag="ACHKFK_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO9054626.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLNKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTAIPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(6325..6483)
                     /locus_tag="ACHKFK_004424"
     CDS             complement(6325..6483)
                     /locus_tag="ACHKFK_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO9054627.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            6666..6965
                     /locus_tag="ACHKFK_004425"
     CDS             6666..6965
                     /locus_tag="ACHKFK_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO9054628.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            6975..7499
                     /gene="ygaP"
                     /locus_tag="ACHKFK_004426"
     CDS             6975..7499
                     /gene="ygaP"
                     /locus_tag="ACHKFK_004426"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO9054629.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKKAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSVFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(7546..7950)
                     /gene="stpA"
                     /locus_tag="ACHKFK_004427"
     CDS             complement(7546..7950)
                     /gene="stpA"
                     /locus_tag="ACHKFK_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO9054630.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFIDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         1827 a         2053 c         2183 g         1913 t
ORIGIN      
        1 cggtgaatat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
       61 gaaaatgctc ggcctcgaac cgggctttat tatctgcccg ttccgtggcg agtatttccg
      121 ccttgcgccg gagcataacc agattgttaa ccacctgatt taccccattc ccgaccccgc
      181 gatgccattt ttgggcgtgc atctcacccg catgatcgat ggcagcatga ccgtcgggcc
      241 aaacgcggtg ctggctttta aacgcgaaga ctatcgcaag cgcgatttct cgtttagtga
      301 cacgctggag attttaggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
      361 aggattgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcggc tggtgcaaaa
      421 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgcaggtg tgcgggcgca
      481 ggcggtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccgcgcac
      541 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
      601 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
      661 agcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
      721 acgtaacaat gaaacttaac gacagtaact tattccgcca gcaggcgttg attaacgggg
      781 aatggctgga cgccaacaat ggcgaggtca tcgacgtcac caatccggcg aacggcgaca
      841 agctgggtag cgtacccaaa atgggcgctg atgaaacccg cgccgctatc gacgccgcca
      901 accgcgctct gcccgcctgg cgtgcgctca ccgccaaaga acgcgccaac attctgcgca
      961 actggttcaa ttttatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     1021 agggcaaacc actggctgaa gcgaaaggcg aaatcagcta cgccgcctca tttattgagt
     1081 ggtttgcaga agaaggcaaa cgtatttatg gcgacaccat tcccggtcat caggccgata
     1141 aacgcctgat tgttatcaag cagccgattg gcgtcaccgc ggctatcacg ccgtggaact
     1201 tcccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggc tgcacgatgg
     1261 tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcagaa ctggcgattc
     1321 gcgcgggcgt tccggctggc gtgtttaacg tagtcaccgg ttcggcgggc gcggtcggta
     1381 acgaactgac cagcaacccg ctggtgcgca agctgtcgtt taccggctcg accgaaattg
     1441 gccgtcagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     1501 gcaacgcacc gtttattgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc
     1561 tggcctcgaa attccgtaac gccgggcaaa cctgcgtctg cgccaaccgc ctgtatgtgc
     1621 agaacggcgt gtatgaccgc tttgccgaaa aattgcagca ggcggtgagc aaactgcaca
     1681 tcggcaacgg gctggagaaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     1741 caaaagtgga agagcatatt gccgatgcgc tggagaaagg cgcgcgcgtg gtttgcggcg
     1801 gtaaagcaca tgaacgcggc ggtaacttct tccagccgac cattctggtg gacgttccgg
     1861 ctaacgccaa agtatcgaaa gaagagacgt ttggccccct cgccccgctg ttccgtttta
     1921 aagatgaagc cgatgtgatt gcgcaagcca atgacaccga atttggcctt gcagcctatt
     1981 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcactggag tacggcatca
     2041 tcggcatcaa caccggcatt atttccaatg aagtggcccc gtttggcggc attaaagcct
     2101 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     2161 tgtgcatcgg tctttaactg gagaatgcga atgagcagca ataaagagtt aatgcagcgc
     2221 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     2281 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     2341 gcggtgctca ataccgggca cctgcatccg aaagtggtgg cggcagtgga agcgcagttg
     2401 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     2461 gagattatga atcagaaggt gccgggcgat tttgccaaga aaacgctgct ggtcaccacg
     2521 ggttctgaag cggtggaaaa cgcagtgaaa atcgcccgcg ccgccaccaa acgcagcggg
     2581 accatcgcct ttagcggcgc gtatcacggg cgcacgcact atacactagc gctgactggc
     2641 aaggtgaatc cgtactctgc ggggatgggg ctgatgccgg ggcatgttta tcgcgcgctt
     2701 tatccttgcc cgctgcatgg catcagtgaa gatgacgcta tcgccagtat ccaccgaatc
     2761 ttcaaaaatg acgcagcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     2821 gaaggtggtt tctacgccgc aacaccagcc tttatgctgc gtttacgcgc gctgtgtgac
     2881 gaacacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     2941 ctgtttgcga tggagcagat gggcgtggcg ccggatctca ccacctttgc gaaatcgatc
     3001 gcaggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga cgccgtagca
     3061 ccgggcggac tgggtggcac ctatgccggt aacccgattg cctgcgtagc ggcgctggaa
     3121 gtgttgaagg tgttcgagca ggaaaatctg ctgcaaaaag ccaacgttct ggggcagaag
     3181 ctgaaagacg gattgctggc gatcgccaaa aaacacccgg agatcggcga cgtacgcggg
     3241 ctgggggcga tgatcgccat cgagctgttt gaagatagcg atcacagcaa accggacgca
     3301 aaactcaccg ccgaaatcgt ggcacgcgcc cgcgataagg gtctgattct tctctcctgc
     3361 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     3421 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagcagta gcaccgcacc
     3481 atgccggagg cgacgctgcg cgtcttgtcc ggcctacgag aatccaggtc ggataaggcg
     3541 tttacgccgc atccgacaat ctgtacgtga acaggaataa atctatgtcg gccggataag
     3601 gcggagccgc tctccggcaa aaagaaccaa taacaattaa acgcgtgacc cgacgcggga
     3661 aatgtcgggg cgctctccca agtgacacac tttcgagagg attcaggatg gggcaatcat
     3721 cgcaaccaca tgagttaggc ggcgggctga agtcacgcca cgtcaccatg ttgtctattg
     3781 ccggtgttat cggcgcaagt ctgtttgtcg gttccagcgt cgccatcgcc gaagcgggcc
     3841 cggcggtatt actggcctat ctgttcgccg gattactggt ggttatgatt atgcggatgt
     3901 tggcggaaat ggcggttgcc acgcccgata ccggttcgtt ttccacctat gccgataaag
     3961 ccattggccg ctgggcgggc tataccatcg gctggttgta ctggtggttt tgggtactgg
     4021 ttatcccact tgaagccaac atcgccgcca tgatcctgca ctcgtgggtt ccaggcattc
     4081 ccatctggtt attttccctc gtcattaccc tcgccttaac cggcagtaat ttattaagcg
     4141 ttaaaaacta cggcgaattt gagttctggc tggcgctgtg taaagtcatc gctatcctgg
     4201 cctttatttt ccttggtgca gtcgcaatta gcggttttta cccgtatgcc gacgtgagcg
     4261 gtatttcaag attgtgggat agcggcggct ttatgcccaa cggcttcggt gcggtattaa
     4321 gcgcgatgtt gatcaccatg ttctcgttta tgggcgcaga aattgtcacc attgccgccg
     4381 cggaatccga cacgccggaa aaacatattg tccgcgccac caactcggtt atctggcgta
     4441 tctctatttt ctatttgtgt tctatttttg tcgttgtggc attaatcccg tggaatatgc
     4501 cagggctgaa agccgttggt tcttatcgct cggttctgga attgctcaat attcctcatg
     4561 cgaaattaat catggactgc gtgatattac tttccgtaac cagctgcctg aactcggcgc
     4621 tgtataccgc ttccaggatg ctctactcct taagccgtcg cggtgatgct cccgcggtaa
     4681 tgggtaaaat caaccgcagt aaaaccccgt acgtggcggt gttactctcc accggcgcgg
     4741 cattcttaac ggtggtggtg aactattacg cgcctgcgaa ggtgtttaaa tttctgatcg
     4801 acagctccgg cgctatcgcc ctgctggttt atttagtcat cgccgtttca caattgcgga
     4861 tgcgcaaaat tctgcgagca gaaggaagcg aaattcgcct gcggatgtgg ctttatccgt
     4921 ggctcacctg gctggtaatc ggttttatta cctttgtgtt ggtagtgatg ctattccgcc
     4981 cggcgcaaca gttagaagtg ctctccaccg gcttattagc gattgggatt atctgtaccg
     5041 tgccaattat ggctcgctgg aaaaagctga taatgtggca aaaaacaccc attcataata
     5101 cgcgctgata atgatcagga gtcacaccat gaccattacc tctctggatg gctatcgctg
     5161 gctgaagaac gatattattc gcggcaattt tcagccggat gaaaaattac ggatgagttt
     5221 gctgacatcg cgttatgcac ttggcgttgg gccgttgcgg gaagcgcttt cgcaactggt
     5281 ggcagaacga ctggtcacgg tggtgaatca aaaagggtat cgggtggcgt ctatgtcaga
     5341 gcaggagctg ctcgatattt tcgatgcccg tgccaatatg gaagcgatgc tggtaagcct
     5401 ggcgattgcc cgcggtagtg atgagtggga ggcagacgtt cttgcgaagg cgcatctgct
     5461 cagtaagctt gaggcctgtg acgccagtga gaaaatgctt gatgagtggg atctgcgtca
     5521 tcaggcgttt catactgcaa ttgtgacagg ctgtggttct cactatttgc tgcaaatgcg
     5581 tgaacggttg tttgatctgg cggcgcgcta tcgcttcatc tggctgcggc gaacggtact
     5641 ttccgtggaa atgctggaag ataaacacaa ccaacatcaa attttaaccg aagctgtgct
     5701 ggcgcgggat gccgcgcgcg ccagtgagtt aatgcgccag catttgctga cgccgattcc
     5761 cattatccag caggcgatgg cttgcaatta actactcttc aggaatacgc aacacttgcc
     5821 ccgggtaaat tttatccggg ctttttagca tcggtttatt cgcttcgaag attttattgt
     5881 acagattagc gttaccgtag acctgtttgg aaatggcact cagagtgtcg ccagacttaa
     5941 cggtataaaa ctggctggca gtggctggta tcgccgtttt cacctgatca tcgacactgg
     6001 caataccgga aatattcccc accgcaacaa ggattttctc cttcgcctcc tgacttaagc
     6061 cgtcaccagt gaccgtcgct ttgccgtcgg caatttgaat gttcacttta tcggcatcag
     6121 gtataccggt tttgttcaga tgctcctgca ccttcttcgc ctgatcgtct ttatcgtgct
     6181 gacctgtaac cgcgtcccag agtttttctc cggcatcttt cacaaaattg aacagaccca
     6241 tagctacctc attcgttaac ggaaacactc agaaagtgta gcagaggaac gcggcgcgtt
     6301 aacgccgcgg gggtgagcac aggtttaatc gcgggtttgc acccagaacg cgtgaatcaa
     6361 accggggata tagccaagca gtgtcagcag aatattaata atgaacgccc aaccgaaccc
     6421 tttaccgagc agcacgccga gcggcggcag aataatggtg atgacgattc tccagaaacc
     6481 catatgtact ccctataaga aaattactca ttgtttaaaa agagatttta tctcttaagc
     6541 gtagtaattt tagcggagcc tgccagtttc agcttacgca tttactctca tttacgcggc
     6601 tgaggctggt atatgttgca tttttagttt agagtttact taatttagaa aatacttaaa
     6661 tatctatgac tgaactcgca caattacagg ccagtgccga acaggcagcg gccttattga
     6721 aagcaatgag ccaccctaaa cggttgctga ttctgtgcat gcttagcggt tcccccggca
     6781 ccagtgcggg agagctgacg cgcattaccg gactaagtgc ctctgcaaca tcacagcatc
     6841 tcgctcgtat gcgggacgag gggcttatcg acagccaacg ggatgcccaa cgcattctat
     6901 attccattaa aaatgaggcg gtaaatgcca ttatcgccac cctgaaaaat gtctattgtc
     6961 cgtaaggagt cactatggct ttgacaacca tttcgccgca tgatgcacaa gaattaatcg
     7021 cacgcggcgc aaagttaatc gatattcgtg atgctgatga atatcttcgt gaacatattc
     7081 ctgaagcaga tctagctcca ttatccgtac tggaacagtc aggtcttccg cctaaattac
     7141 gtcgcgagca aattatattc cactgccagg caggtaaacg caccagtaat aacgccgata
     7201 aattagcagc gattgccgcc cccgcagaaa tctttttact cgaagatggg attgatggct
     7261 ggaaaaaagc aggattgcca gtagcggtaa ataaatccca acccttgccg ttaatgcgcc
     7321 aggtgcagat cgccgcgggt ggtttaatat taatcggcgt tgtactgggt tataccgtaa
     7381 atagcgtttt cttcttatta agcggctttg ttggtgctgg gttactgttt gcaggaatta
     7441 gtggtttttg tggaatggca aggttgttag ataagatgcc gtggaaccaa cgagcttgag
     7501 aagcgacgcc ggacgcgcca tagcagcgac atccggcctc agtaattaga tcaggaaatc
     7561 gtcgagagat ttaccttctg ccagcgcctg agcgattggc ttcggtgtac ggccctgacc
     7621 ggtccaggtt ttagtttcac cgttaacatc gatgaattta tatttcgccg gacgcggctg
     7681 gcgtttttta ccagcgcgtg gtgccgcagc agagctatta cccaataact cttccgggtt
     7741 aattccgtca gctttcatca gctccagcca ggtgctaatt ttttcctggc gctctgccag
     7801 ttcacgctgc tgttgttctt cttcttcacg tctttcttta gtgacaaccc tgaatttttc
     7861 gagcatttct tcaagaacgt caatggagaa ttcgcgagcc atcgcacgga gggtgcgaat
     7921 gttatttaaa ctttgtaaca ttacggacat aataaagaaa accttttaac ccaaaa
//
LOCUS       DAZGJT010000161         7894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZGJT010000161 DAZGJT010000000
VERSION     DAZGJT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.164"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            108..938
                     /locus_tag="ACHKFK_004428"
     CDS             108..938
                     /locus_tag="ACHKFK_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO9054631.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKQLEQALQHFTITHSILEISHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            970..1905
                     /locus_tag="ACHKFK_004429"
     CDS             970..1905
                     /locus_tag="ACHKFK_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO9054632.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFTRLIIERLRHEGVITNGIHVMNNAVTGTAFVSYQNPQLRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVAGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQY"
     gene            1998..2240
                     /gene="yhdT"
                     /locus_tag="ACHKFK_004430"
     CDS             1998..2240
                     /gene="yhdT"
                     /locus_tag="ACHKFK_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO9054633.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            2230..3681
                     /gene="panF"
                     /locus_tag="ACHKFK_004431"
     CDS             2230..3681
                     /gene="panF"
                     /locus_tag="ACHKFK_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO9054634.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVDTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            3693..4574
                     /gene="prmA"
                     /locus_tag="ACHKFK_004432"
     CDS             3693..4574
                     /gene="prmA"
                     /locus_tag="ACHKFK_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO9054635.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            4903..5868
                     /gene="dusB"
                     /locus_tag="ACHKFK_004433"
     CDS             4903..5868
                     /gene="dusB"
                     /locus_tag="ACHKFK_004433"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO9054636.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            5894..6190
                     /gene="fis"
                     /locus_tag="ACHKFK_004434"
     CDS             5894..6190
                     /gene="fis"
                     /locus_tag="ACHKFK_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO9054637.1"
                     /translation="MFEQRVNSDVLTVSIVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            6276..7160
                     /gene="yhdJ"
                     /locus_tag="ACHKFK_004435"
     CDS             6276..7160
                     /gene="yhdJ"
                     /locus_tag="ACHKFK_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO9054638.1"
                     /translation="MRTGCEPTRFGNEAKTIIQGDALTELKKLPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQYRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     NYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            7244..7423
                     /gene="yhdU"
                     /locus_tag="ACHKFK_004436"
     CDS             7244..7423
                     /gene="yhdU"
                     /locus_tag="ACHKFK_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO9054639.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFHDRM"
     gene            complement(7426..>7894)
                     /gene="acrS"
                     /locus_tag="ACHKFK_004437"
     CDS             complement(7426..>7894)
                     /gene="acrS"
                     /locus_tag="ACHKFK_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO9054640.1"
                     /translation="QPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAKIPRQQAL
                     LKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCIANNLDLDVVMIIID
                     GAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELSVI"
BASE COUNT         1946 a         1911 c         1991 g         2046 t
ORIGIN      
        1 tcgttgcaat ttttgacccc tgattttctt ttcgaattgt cgatatgatt gaaggataac
       61 tttcaaagaa aaatacaagg ggctatagtg ttacgcatta aaggccaatg aagagtgtta
      121 acgtgccgag aaaatcgtct aacctactga aacgcagatt aaaaattgca gaaattgttg
      181 ccgcccaggg tgaaatcaaa gttgatgact tatctgcgca acttggtgtc tctggcgtga
      241 cgatccgcgg cgatctcagt tatctggagc aacagggata tttaaagcga tcatttggcg
      301 gtgctattgc gacaccactc tcctctgaac cagaagcgat acaaccgatc gcgccattgt
      361 ctctggcaca acaaatggaa atagcacgac attgcgcacg gttaattcgc gatcgtgaca
      421 cgctatttct tggacatggg acaatctgcc gtaagatcat tccattgctc agcgaagtga
      481 aaaaactgcg tctgatcacc aacgatcccg aacatgcgct gctggcaaat cagtttattg
      541 atggcgaaat aatactggct ggcagtgaaa tgttgcgccc cgacaccgca ctggccggaa
      601 aacagcttga acaggcactt cagcatttca ccattacaca tagcattctg gaaatcagcc
      661 atctggatgc tgatggtact ttaaatatca acgttcccag actcgccgct gcctggcagc
      721 tctgtttcga tcatgcccag aataagacgg ttatcgttgc cgccgatcct gcgccatcaa
      781 cggctgctat cgggcatttg catcaggcag aaaatgtgat cgtcagccgc agcattaacg
      841 aacaatatca gcaacaattg cagtcggctg atttcgttat cctttatacc agtaatgagt
      901 gctttacctg gtccaatcgc gaaaggcttc aggaatgaga ggggttttgt gacaataact
      961 atctgcgcta tgggcgaatt gctggccgag tttttgtccc gcaacccaca tcaaaaattc
     1021 actcagcctg gggagtttat cgggccattt cccagcggtg cgccagcaat ttttgctgct
     1081 caggtggcaa aactgtccca tcgggccatc ttctttggat gtgttggtaa tgatgatttt
     1141 acccgactca ttatagagcg tctccgtcat gaaggtgtca ttaccaatgg gatccatgtt
     1201 atgaacaatg ccgtcacagg tacggcgttc gtgagttatc aaaatcccca gctgcgggat
     1261 ttcgtcttta atatccctaa cagcgcctgc ggtttgttta ctgccgagca cattgataag
     1321 gatctgctta aacagtgtaa ccatctgcat attgtgggct catcgttgtt ctcatttcgc
     1381 atgatcgatg tcatgcgtaa agcaataacg acgatcaaat cggctggcgg caccgtttct
     1441 ttcgatccca atattcgcaa agagatgctg agcattcctg aaatggcgca ggctctcgat
     1501 tatttgattg aatatacgga tatttttatc cccagcgaaa gcgaactccc tttcttcgcg
     1561 cgtcacaaaa atctgtcaga ggaacagatt gttagcgatc ttctccacgg cggcgtaaaa
     1621 catgtggcga taaaacgcgc ccagcgtggg gccagctatt acaagcttaa aaacggtaca
     1681 ttacacgccc agcatgttgc aggtcacgat atcgaaatta tcgatccaac gggtgcaggc
     1741 gactgctttg gcgcaacgtt tatcactctt ttcttatccg gtttcccggc acacaaggcg
     1801 ctgcaatatg caaatgccag cggcgcgctc gccgtaatgc ggcaaggtcc gatggaaggg
     1861 atatcctcac tggcagacat tgaagacttt ttgcagcagt actgaacggc aattttctgt
     1921 cagatcattt tccctgcctg aagacttgcc gtagaattgc ccgtttctcg tatcccccca
     1981 tgaggaggac cccttccgtg gacactcgtt ttgttcaggc ccataaagag gcgcgctggg
     2041 cgctggggct gacccttttg tatctggcgg tatggttagt agccgcttac ttacctggcg
     2101 ttgcccccgg ttttaccggc tttccgcgct ggtttgagat ggcctgcatc ctgacgccgc
     2161 tgctgtttat tggactgtgc tgggcgatgg tgaaatttat ctatcgcgat atcccactgg
     2221 aggatgacga tgcagcttga agtaattcta ccgctggtcg cctatctggt ggtggtgttc
     2281 ggtatctctg tttatgcgat gcgtaaacgg agcactggca ccttccttaa tgagtatttc
     2341 ctcggcagcc gctctatggg cggtattgtg ctggcgatga cgctcaccgc gacctatatc
     2401 agtgccagtt cgtttatcgg cgggcctgga gctgcttata aatacggcct gggctgggta
     2461 ttgctagcga tgattcagct tcctgcagtc tggctttcgc tcggtattct cggcaagaag
     2521 ttcgcaattc ttgcgcgccg ctacaatgcg gtgacgctga acgatatgct gtttgcccgc
     2581 taccagagtc gtcttctggt atggctggcg agtttgagtt tgctggttgc gttcgttggt
     2641 gcgatgaccg tgcagtttat cggcggtgcg cgcctgctgg aaaccgcggc gggtattcct
     2701 tatgaaaccg ggctgctgat tttcggtatc agcattgcgt tgtataccgc ctttggcggc
     2761 tttcgcgcca gcgtgctgaa cgacaccatg caagggctgg tgatgctgat tggcaccgtt
     2821 gtgctgctta ttggcgtggt acatgccgca ggtggtttaa gtaatgcggt agacacgctg
     2881 caaaccatcg atccgcaact ggttacgcca caaggcgctg acgatattct gtcgcctgcc
     2941 tttatgacgt cgttctgggt actggtgtgc tttggcgtta taggcctgcc gcatactgcg
     3001 gtgcgctgta tctcttataa agacagcaaa gccgtacatc ggggaatcat tatcggtacg
     3061 attgtagtcg caatcctgat gttcggtatg catctggccg gagcgttagg tcgggcggtg
     3121 atccccgatc tcaccgtacc ggatctggtg atcccaacgt taatggtaaa agtgctgcca
     3181 ccgtttgctg ccgggatctt cctggctgcg cctatggctg cgatcatgtc gacaattaac
     3241 gcccaactgc tgcaaagttc cgctacgatc attaaagatc tctatctgaa tatccgtccg
     3301 gatcaaatgc aaaacgagac gcgtctgaag cggatgtcgg cggtgatcac gttggttctc
     3361 ggtgcgttgc tgctgcttgc cgcctggaag ccgccagaaa tgatcatctg gctgaatttg
     3421 ctggccttcg gtgggctgga agccgttttc ctgtggccgc tggtgctggg tctttactgg
     3481 gaacgcgcca acgccaaagg cgcgctaagt gcgatgatcg ttggcggcgt gctgtatgcc
     3541 gtactcgcga cgctgaatat tcagtacctg ggcttccacc ctatcgtgcc ctcgttacta
     3601 ctaagtttgc tggctttcct ggtcggaaac cgtttcggta catccgtccc gcaagctacc
     3661 gttttgacta ctgataaata aagagttttg ccatgccttg gatccaactg aaactgaaca
     3721 ctaccggcgc gaacgcggaa gatcttagcg atgcgctgat ggaagcgggt gccgtttcta
     3781 tcacttttca ggatacccac gatacgccag tatttgagcc gctgccgggc gaaacgcgcc
     3841 tgtggggcga caccgatgtg attggtctgt tcgacgctga aaccgatatg aacgacgtgg
     3901 tggcgattct ggaaaaccat ccgctgctcg gcgcgggctt cgcgcataaa atcgaacaac
     3961 tggaagataa agactgggag cgcgaatgga tggataattt ccacccgatg cgctttggtg
     4021 aacggctgtg gatctgccca agctggcgtg atgtgccgga cgaaaacgcc gtcaacgtga
     4081 tgttagaccc agggctggcg tttggtacgg gtacccatcc aactacctct ctgtgcctgc
     4141 aatggctcga cagcctcgat ttaaccggta aaacggtcat cgactttggc tgtggttccg
     4201 gcattctggc tatcgcggcg ctgaagctgg gtgcagcaaa agccattggt attgatatcg
     4261 acccgcaggc gattcaggcc agccgcgaca acgccgaacg taatggcgtt tctgaccgtc
     4321 tggaactcta cctacctaaa gatcagccag aagaaatgaa agccgacgtg gtggtcgcta
     4381 acatccttgc aggcccatta cgtgaactgg caccgttaat cagcgtcctg ccggtttcag
     4441 gtggcttgct gggcctttcc ggtattctgg caagccaggc agagagcgtt tgtgaagctt
     4501 atgccgacag cttcgctctg gacccggtcg tggaaaaaga agagtggtgc cgcattaccg
     4561 gtcgtaagaa ttaaccttcg catcgccgta gggtgacgca aggcgaagtg cgagcaaggt
     4621 cacaaaaggc acgttaattt gccgattatt tacgcaaatt tgcgtgccaa aattttcatt
     4681 cataaagaaa aattgagaac ttactcaaat ttcttggagt gtaaatttta gtcactattt
     4741 tctaatataa tgatttttat gagtaattat cgcgccaagc tcattttaaa tgcaattctt
     4801 tgatccatct cagatgattg gtcaaagttt ggcctttcat ctagtgcaaa aaatgcgtaa
     4861 tatacgccgc cttgcagtca cagtatggtc atttcttaac tcatgcgcat cggacaatat
     4921 cagctcagaa atcgcctgat cgcagcgccc atggctggca ttacagacag accttttcgg
     4981 acgttgtgct acgagatggg agccggattg acagtatccg agatgatgtc ttctaaccca
     5041 caggtttggg aaagcgacaa atctcgttta cggatggtgc acattgatga acccggtatt
     5101 cgcaccgtgc aaattgctgg tagcgatccg aaagaaatgg cagatgcagc acgtattaac
     5161 gtggaaagcg gtgcccagat tattgatatc aatatgggtt gcccggctaa aaaagtgaat
     5221 cgcaagctcg caggttcagc cctcttgcag tacccggatg tcgttaaatc gatccttacc
     5281 gaggtcgtca atgcagtgga cgttcctgtt accctgaaaa ttcgcactgg ctgggcaccg
     5341 gaacaccgta actgcgaaga gattgcccaa ctggctgaag actgtggcat tcaggctctg
     5401 accattcatg gccgtacacg cgcctgtttg ttcaatggag aagctgagta cgacagtatt
     5461 cgggcagtta agcagaaagt ttccattccg gttatcgcga atggtgacat tactgacccg
     5521 cttaaagcca gagctgtgct cgactataca ggggcggatg ccctgatgat aggccgcgca
     5581 gctcagggaa gaccctggat ctttcgggaa atccagcatt atctggacac tggggagttg
     5641 ctgcccccgc tgcctttggc agaggttaag cgcttgcttt gcgcgcacgt tcgggaactg
     5701 catgactttt atggtccggc aaaagggtac cgaattgcac gtaaacacgt ttcctggtat
     5761 ctccaggaac acgctccaaa tgaccagttt cggcgcacat tcaacgccat tgaggatgcc
     5821 agcgaacagc tggaggcgtt ggaggcatac ttcgaaaatt ttgcgtaaac agaaataaag
     5881 agctgacaga actatgttcg aacaacgcgt aaattctgac gtactgaccg tttctatcgt
     5941 taactctcag gatcaggtaa cccaaaaacc cctgcgtgac tcggttaaac aggcactgaa
     6001 gaactatttt gctcaactga atggtcagga tgtgaatgac ctctatgagc tggtactggc
     6061 tgaagtagaa cagcccctgt tggacatggt gatgcaatac acccgtggta accagacccg
     6121 tgctgctctg atgatgggca tcaaccgtgg tacgctgcgt aaaaaattga aaaaatacgg
     6181 catgaactaa ttcaggttag ctaaatgctt gattaaaaag gcgctactcg gcatggggaa
     6241 gcgccttttt tataggtgtc acaaagggag tgaccatgag aacaggatgt gaaccgaccc
     6301 gatttggtaa tgaagcaaag accatcatcc agggtgatgc attaaccgaa cttaaaaagc
     6361 tccccgccga aagtgtcgat ctgatctttg ccgacccacc gtataacatc ggtaaaaatt
     6421 ttgatggtct gatcgaagcc tggaaagaag atctgtttat cgactggctg tttgaagtga
     6481 ttgcagagtg ccatcgcgtt ctgaaaaagc agggcagcat gtacatcatg aatagcacgg
     6541 aaaacatgcc ctttatcgat ctccagtacc gcaagctttt taccatcaaa agtcgcatcg
     6601 tctggtcata tgacagttct ggagtgcagg cgaaaaaaca ctacggctcc atgtacgaac
     6661 ccatcctgat gatggtgaaa gacgcaaaga actacacgtt caacggtgat gctattctgg
     6721 tagaagccaa aaccggatcg cagcgcgcgt tgatcaatta tcgcaaaaat cctccacagc
     6781 cttacaatca tcaaaaagta ccgggtaacg tctgggattt tccgcgcgtg cgttatttga
     6841 tggatgaata tgaaaaccac ccgacgcaaa aaccggaagc cttgctgaaa cgcattattc
     6901 tcgcctcttc caacccaggc gatatcgttc tcgacccgtt tgccggtagc tttactaccg
     6961 gtgccgtagc cgtcgccagc gggcgaaaat tcattggtat tgagatcaac agcgagtaca
     7021 tcaaaatggg gcttcgacgg ctggatgtcg cgtcgcatta ctctgcggaa gaactggcga
     7081 aagtgaaaaa aagaaagacc ggcaacctgt caaaacgaag ccggttaagc gaagttgacc
     7141 ccgatctcat tgcaaagtaa aggaagtgta agcctggttt tcagattatt catttcgtgt
     7201 atatttcctg ccagacttgg ttaaacatgc acaggctcag gtaatgattc gcaagtattg
     7261 gtggctcgtc gttttcgctg tcttcgtttt tctgtttgat actttactga tgcaatggat
     7321 tgaactgctg gcaacagaaa cagacaaatg ccgcaatatg aactcagtta atccactaaa
     7381 actggttaac tgtgacgaac tgaattttca cgacagaatg tgaatttata tgacacttaa
     7441 ttcattcgtt tgatgaatta atttcgttat gttttcatct ggcatgaaca ttcttaatac
     7501 gttatcgacc agagcggggg cttgtttata aagatcataa cccgccatat tcattaacca
     7561 gttttgaaca attccgctga aggcaccatc aataataatc atcacaacat ctaaatcgag
     7621 attatttgct atacaacctt gttgctgaca cgcctgcaat acttcgcgga gagtctgcgg
     7681 attaaagccc atcttttcgc gtatcactcc ctcggccagc atctcatcat taaattcaca
     7741 tttgtgatat aagattttca gcaacgcctg ctggcgggga attttggcaa tatattgcaa
     7801 accgacaatc aatttttcac gcaattgctg aaacgggtca tgctctaacc cagcggtcaa
     7861 gtgatcctgg attaactccc gcaatgaagg ctgt
//
LOCUS       DAZGJT010000162         7803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZGJT010000162 DAZGJT010000000
VERSION     DAZGJT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.165"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..444
                     /locus_tag="ACHKFK_004438"
     CDS             <1..444
                     /locus_tag="ACHKFK_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrating conjugative element protein"
                     /protein_id="HFO9054641.1"
                     /translation="QFSAAITGKESEVVSPLLMDVNPSLTMENISELSTSSEPSQQGV
                     FPVICTRLHPGSVMKRQLLTGWGPVFIIGDDPFSLRWMSEHLEILKSLNALGLVVNVE
                     SVERMEVLQQRADGLLLLPVICDNFVQALQLNAYPVLITEMEISQ"
     gene            470..664
                     /locus_tag="ACHKFK_004439"
                     /pseudo
     CDS             470..664
                     /locus_tag="ACHKFK_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(715..1485)
                     /gene="yafV"
                     /locus_tag="ACHKFK_004440"
     CDS             complement(715..1485)
                     /gene="yafV"
                     /locus_tag="ACHKFK_004440"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO9054642.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESDSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNERVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYKEKFPAWRDADEFRLW"
     gene            1666..2112
                     /gene="ivy"
                     /locus_tag="ACHKFK_004441"
     CDS             1666..2112
                     /gene="ivy"
                     /locus_tag="ACHKFK_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO9054643.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(2155..4599)
                     /gene="fadE"
                     /locus_tag="ACHKFK_004442"
     CDS             complement(2155..4599)
                     /gene="fadE"
                     /locus_tag="ACHKFK_004442"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO9054644.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAVLGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKNHYLLRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAYIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4839..5417
                     /gene="lpcA"
                     /locus_tag="ACHKFK_004443"
     CDS             4839..5417
                     /gene="lpcA"
                     /locus_tag="ACHKFK_004443"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO9054645.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5622..6389
                     /gene="yafJ"
                     /locus_tag="ACHKFK_004444"
     CDS             5622..6389
                     /gene="yafJ"
                     /locus_tag="ACHKFK_004444"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO9054646.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGESVV"
     gene            complement(6360..7100)
                     /gene="dpaA"
                     /locus_tag="ACHKFK_004445"
     CDS             complement(6360..7100)
                     /gene="dpaA"
                     /locus_tag="ACHKFK_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO9054647.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            7412..>7803
                     /locus_tag="ACHKFK_004446"
     CDS             7412..>7803
                     /locus_tag="ACHKFK_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
                     /protein_id="HFO9054648.1"
                     /translation="MSSFLHGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAKSRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSDHLHNITEVAIHRLEQQLG"
BASE COUNT         1927 a         2049 c         1935 g         1892 t
ORIGIN      
        1 cagtttagtg cagccattac tggcaaggaa agtgaggttg tttcaccatt attaatggat
       61 gttaatcctt cactaacaat ggaaaatatt tctgaattat ccacatcatc ggaaccatcg
      121 caacaggggg tatttcctgt aatatgtact cgacttcatc ctggctccgt aatgaaacgt
      181 cagttattaa ccggctgggg gcctgttttt ataattggtg atgatccttt ttctctgcgc
      241 tggatgagtg aacatctgga gatccttaaa tcgctaaatg cccttggttt ggttgttaat
      301 gttgaatcag tagaacgaat ggaggtatta cagcagcggg ctgatgggtt attgttattg
      361 ccagttatct gcgataattt tgtgcaagct ctgcaattaa atgcttatcc ggtattaatc
      421 acagagatgg aaatatcaca atgattaata acataattac tgctaataat atggtgattt
      481 tgaaaatggt aatcctgttc acgacaccat aatcagagtt atgtcctgta tccgttcagt
      541 aaaatttcac gaatgcttta ttaaccggat gcgtgaatgt cattcttcag acgacataga
      601 cgtcattgcg attgatggaa aagcacttcc acactcctgt gataaaagtc gtcgcaggag
      661 agcgggcttt cgtagtcttc cccgactctc cccggcccta aacacaaccc ccactcacca
      721 caacctaaac tcatccgcat cccgccatgc cggaaacttt tctttatatt cccgcaatgc
      781 catcatcgac agctccgcat caatgcgcgt tgcctgatgc gcgtcggcag tagcgataat
      841 ctcgccttgt ggattaatca cccggctgtc accgcgataa tggcagccgt tgccatcgct
      901 gccgacgcga ttacatcccg ctacatacgc ctgattctca atcgcgcggg ctgtcagcaa
      961 tgcctgccag tgcagagaac gcggtgcagg ccagttggca acgtagatgg cgaggtcata
     1021 atcgttgaga ttgcgcgacc acaccggaaa acgtaagtcg tagcacacca gcggtaaaat
     1081 acgccagccg cgccattcca caatcactcg ctcattgccc gctttataat gcagatgctc
     1141 atctgccatg cggaacagat gacgcttatc ataaaaatgt accgtgccgc ccggctcaac
     1201 cagcaaaaag cggttaaccg aatcagactc cgtttgtaat gcaacactgc ctgcaatcag
     1261 cgcattgcac tgctgcgctt tagcggtcat ccagttcact acgtcatctt gtgctagcga
     1321 cgaagctgcc gcttccatgg caaagccgct ggtaaacatc tccggtagaa cgatcacatc
     1381 gcgcccggta ataccttcca gttgacgatc aaaatggcgc aggttggcag gaccatccat
     1441 ccacaccagt ggttgctgca aaagcgtaat cttcaaacca ggcacggtgt acaactcctt
     1501 tatgcgaagg gttttataac tctaacacct tatcaggcag ttgccttagc gcagaataaa
     1561 ttgataacaa atgctgatat tggaaatatc tgatttgcaa attatcgtgt tatcgccagg
     1621 ctttagggag ttaatcacat gggcaggata agcccgggag gaatgatgtt taaggcaata
     1681 acgacagtcg ccgctctggt catcgccacc agtgcaatgg cgcaggatga tttaaccatt
     1741 agcagtcttg caaagggcga aaccaccaaa gcggcgttta atcagatggt gcaagggcat
     1801 aagctgcctg cctgggtgat gaaaggcggt acttatactc ccgcacaaac cgtgactttg
     1861 ggagatgaga cgtatcaggt gatgagcgcg tgcaaaccgc atgactgtgg ctcgcaacgt
     1921 atcgctgtga tgtggtccga gaaatctaat cagatgacgg ggctgttctc gactattgat
     1981 gagaaaacgt cgcaagagaa actcacctgg ttgaatgtga acgatgcgct ttcgattgat
     2041 ggcaaaacgg tgctgttcgc ggcgttgacc ggcagcctgg aaaaccatcc ggatggcttt
     2101 aattttaaat aattagccga taaaaaaacg gagccgaaag gctccgttat tcaattacgc
     2161 ggcttcaact ttccgcactt tctccggcaa ctttaccggc ttcgtcgcca gctcttctgg
     2221 atcaaagtca tcaacgttaa tactgcgtaa acggctttct tcagctttta ccagaatagc
     2281 ggcttcatct ttatcaatca gccccttcgc cagcgcgttg tgcgccagtt catccagacg
     2341 ggtaaacggc aggtttttac ccaactcttt acagatccgc tgatgaatcg ggtcggcggc
     2401 aatcacatcc accagcgcct cttccagtaa accaaccggg ttatgctcgc ttggcgtcag
     2461 gtactgaccg cgaccaatac gtgaacgagt ggcgttcggt acttgtaaaa tcttcgccac
     2521 tttatgatcc agcttgtcag acggtgccag gtaatgacgt ccggtcggga agatcaccac
     2581 attcagcagc ccggcaacca cacggttcgg gaagttttgc agtaaatcgt ccatcgcctg
     2641 ttctgcctga tacagcgcat cctgtacgcc ccagtgcacc agcggcagat cggcttcatt
     2701 acggccttcg tcgtcataac gcttcagaac ggcagaggcg aggtaaagct ggcttaaaat
     2761 atcccccaga cgggccgaga tacgctcacg acgtttcagg ctgccgccca gcaccgccat
     2821 cgagacatca gaaagcaggg cgaggttggc gctcaggcgg ttcaggtgct gatagtagcg
     2881 tttagtggca tcgccggttg gtgtgctgct ggttaaaccg cgcgtcaggc ccagccagaa
     2941 gctgcgaact ttgttgctac cgacgtgacc gatatgtttg aacagcagtt tatcgaacgc
     3001 atttacgtca ttgttcttcg ccgcttccat ctcttccagc acgtacggat ggcaacgaat
     3061 tgctccttgt ccgaagatca tcatgctgcg ggtcagaatg ttagcccctt caacggtgat
     3121 ggcaatcggt gcgccctggt aagcacgggc caggaagttg ctttgcccga gcataatgcc
     3181 tttgcccccg gtgatatcca tcgcatcaat aatcgactgc tgcccgcggt gggtacagtg
     3241 atacttaacg atagccgaca gcacggcagg tttttcaccg agcataatgc cgtaggtaat
     3301 cagcgatgcc gcagcatcca ttacataagc gttcccggca atacgcgcca gcggctcttc
     3361 aatcccttcc atcttaccga tagagatttt gaactgccga cgaatgtacg catacgcgcc
     3421 cgttgccagc gctaccgatt tcacgccgcc ggttgagttg gaaggcaggg tgatgccgcg
     3481 gcctaccgag aggcactcca ccagcatccg ccagccttgc ccggccattt tcggcccgcc
     3541 gatgatgtaa tcgatcggca cgaacacatc tttaccgcgc gtcggtccgt tctggaacgg
     3601 tacgttcagc gggaagtggc gacgaccaat ttccacgccc ggcgtggtgg tcgggatcag
     3661 cgcacaggta atacctaaat cttctgcgcc acccagcagt ttctccgggt cggagagttt
     3721 aaacgccaga ccaagcacgg tcgcaatcgg tgccagcgta atgtagcgtt tgttccaggt
     3781 cagacgcatc cccagcacct gctggccctg ccattcgccc atgcagacaa tcccggtatc
     3841 cggaatcgcg cccgcatcgg aacccgcttc cgggctggtc agtgcaaagc aagggatctc
     3901 ctgaccacgc gccagacgca gcagatagtg atttttctgc tcgtcagtgc cgtaatgttg
     3961 cagcagctcg cccgggccta atgagttcgg cacgccgacg gtaatcgcca ggatcccgct
     4021 cacgccggag agtttttgca gcacgcgaga ctgggcataa gccgagaact ccagcccgcc
     4081 gtactctttt ttgatgatca tcgcgaagaa acgatgctct ttaaggtacg cccacaactc
     4141 cggcggcagg tccgccagct catgagtaat ctggaaatca ttcgccatcc ggcaggcttc
     4201 ttctaccgga ccgtcgagaa acgcttgttc ttcagcggtc aggcgcggct gcggatagtt
     4261 atgcagcttt ttccagtccg gcttgccctg gaacaagtcg ccctcccacc aggtggtgcc
     4321 cgcatcaatc gcttctttct cagtgcgcga catcggcggc atcaccttac ggaaaccgcg
     4381 aaataccggt gcggaaatca tcgacttacg cataggcgca aagttaaatg gcacgaggat
     4441 aatggccaga ggcactagta cccacgccga ccacagacca gcaacgccga ggacagctgt
     4501 ccaggcgagc aaaattagac tgctgataaa taagctcacg cggtgataga acaatgcgcc
     4561 gagcaggaca accgtagcga gaatactcaa aatcatcata acgaaaagcc ccttacttgt
     4621 aggaggtctg accacttgtg atgatatggt tgtagtggat gtgaaaatat ttagcaatgt
     4681 gtttacaata taattacaac aaagctcaca ttgttgctgt ttttatccgc acttcaggtc
     4741 aaaaagtcct ggtcatagca cctgcccgta cttctcgctt ttggcggtat ccggtacact
     4801 gcattttgtc tattacattt atgctgaagg atatcctcat gtaccaggat cttattcgta
     4861 atgaactgaa cgaagcggcg gaaacgctgg ctaacttttt aaaagatgac gccaatattc
     4921 acgccattca gcgcgcggcg gtcctgttag cagacagctt taaagccggt ggcaaagtgc
     4981 tttcctgtgg caacggcggc tcccattgcg acgccatgca ctttgccgaa gagttgaccg
     5041 gtcgctaccg tgaaaaccgt ccgggctacc cggcgattgc catttctgac gttagtcata
     5101 tttcctgcgt cggtaacgat tttggtttca atgatatttt ctcccgttac gttgaagcgg
     5161 taggtcgcga aggcgatgta ctgctgggga tctctacctc cggtaactct gcaaacgtga
     5221 tcaaagcgat cgcagcggcg cgtgagaagg ggatgaaagt gatcaccctg accggtaaag
     5281 atggcggcaa aatggctggc acggcggata ttgaaattcg cgttccgcac tttggttatg
     5341 ccgaccgcat tcaggagatc cacattaaag tgatccatat cctgatccag ttaattgaaa
     5401 aagagatggt taagtaagtc tggcgtaggc cggataaggc gtttacgccg catccggcat
     5461 ttgtgctctg atgcctgatg cgacgctgac gcgtcttatc atgcctacaa tctgcacccg
     5521 aaccgtaggc cgaataatgc gttcacgcca catccgacct gaaaattctt aaatcaatct
     5581 tcgccggggg ccatgcgctc ccgctgttgt ggaggttacc catgtgcgaa ttgctcggga
     5641 tgagtgccaa cgtccctacc gatatctgct ttagtttcac cggacttgta cagcgtggtg
     5701 gtggaaccgg gccacataaa gatggctggg gcattacctt ttacgaaggt aaaggctgtc
     5761 gcacatttaa agatccgcaa cccagcttta attcccccat cgccaaactt gtccaggact
     5821 acccgataaa atcctgttcg gtggtggctc atattcgcca ggctaatcgg ggcgaggtgg
     5881 cgctggaaaa tactcacccg tttacccgcg agttatgggg gcgtaactgg acttatgccc
     5941 ataatggaca actgacgggc tacaaatcac tggaaaccgg caacttccgc ccggtcggtg
     6001 aaaccgatag cgaaaaagcc ttttgctggc tcctgcataa attaacgcag cgttacccgc
     6061 gcacaccggg caacatggcg gcagtgttta aatatatcgc ctcactggcg gatgaactgc
     6121 ggcagaaggg cgttttcaac atgctacttt cggatgggcg ctatgtaatg gcgtattgct
     6181 cgactaattt acactggatc acccgccgcg cgccgtttgg cgtggcaacg ttgctggatc
     6241 aggatgtgga aatcgacttc agctcgcaga ccacaccgaa tgatgtggtc acggtgattg
     6301 cgacacagcc gctgacgggc aatgaaacct ggcaaaagat tatgccaggt gaatggcgct
     6361 tattttgcct cggggagagt gtagtttgat gccagttgtg gctgcacaac ttcgtggctt
     6421 aacggcttgc tgaccacgta acggccattg accacagaaa cggtcggtgg cttacgggtt
     6481 tgctcaaagt agtcgtagcc tggcttcagt tgctcccaga agtccttaaa gttggaatat
     6541 ttatggcgct tcatattggc gtcggtcata cggaacgggt aaatactcac ttgcacgctc
     6601 ggctgaccaa acaccagcgc accagtaacg aactggaaga tctcatcaat accctgattg
     6661 gtcatcgcgt agcagccgat ggaaacacaa tcgccgtgaa tcatcaggta tttcccttca
     6721 taaccatgcg cacggtcata ggcgtttggg aatccaatat taatcgcttt gtagtaacgg
     6781 ctgtctggtt ttaactgatt acgctggacg ctataaaacc cttccgggct tttgaaatcg
     6841 ccctgacgct gttttggccc taagccgccg gaatatttac agattttata gctgtcgagc
     6901 agttgatatt gctcgcccat tttgacgtag agatcgagcg tacgttcttc cttgaagatc
     6961 tggatgtaga caggggatcc catcaactgc tgcttatact ctttgctcac tggcgtggtc
     7021 gagctactgc tgcccagcaa accggcaaac gaaacgcacg ggatcaacaa catcgcaaga
     7081 attaatgcga ttttacgcat actgcttatt ccttgataaa acggttacac acgccaggac
     7141 ggcaaaatga atcccaaatc ggaatagtct ggatttggaa ggctcacatt atcaccaaaa
     7201 gagttttacg caagcctgtc ggcgcagggt ttacaaattt agcacagggg cagttttcaa
     7261 gagtccgaaa agcgcatgga ttgtagattt atttcttatt tcctaagatt atgaaaccgc
     7321 aatagtaata tgcaaataag caattaataa tcccgtttac tcctccggtc ttttaaaatt
     7381 ctcaccttca ttcagctggt ttatgtcctt catgtcttcg ttcttgcacg gcagatttct
     7441 gcatccaggc gtgttttcgt tatgcgtttt gctcccttta ctcgccagcg ccaccacatc
     7501 acatatctct tttagctacg ccgcccgcca gcggatgcaa aaccgtgcgc gtttattaaa
     7561 acagtaccaa acccatctga aaaagcaggc cagctatatt gtggaaggca atgccaaaag
     7621 cagaagggcg ctgcgccagc ataatcggga gcagataaaa cagcatccag aatggtttcc
     7681 tgcgccgctc aaggcgagtg acagacgctg gcaggcgttg gtggagaata atcatttctt
     7741 aagcagcgac catctacata acatcaccga agtggcgatt caccgcctgg agcagcagct
     7801 agg
//
LOCUS       DAZGJT010000163         7707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZGJT010000163 DAZGJT010000000
VERSION     DAZGJT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.166"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHKFK_004447"
     CDS             <1..159
                     /locus_tag="ACHKFK_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO9054649.1"
                     /translation="SIRHSLRQSSDTVLPALHNLAVAIEHYNENHPHSALGYCSPREY
                     RRQWVTLT"
     gene            309..1307
                     /locus_tag="ACHKFK_004448"
     CDS             309..1307
                     /locus_tag="ACHKFK_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO9054650.1"
                     /translation="MKKVLCALGLMFTAVSSALATTYPLTIENCGYQETFTRPPERVV
                     ALGQNTVEILLLLGLQKQVVASAFWPTSVLPQLAEQNAKIKTLTVEIPSLESVLAQNP
                     DFVPAQLPLLLGPESKVARREDLATVGVNSYVSPGMCATKKATGDMYGSRQKLWDMTW
                     LYQEISDFSRIFNIEDRGQALIADFKKREADLRQEFGKSKKDLSFVFWFSSASPSADA
                     YVGGKNSASGFIASVLGGHNAITSETEWPTVSWESIIAANPDVIVVASLDRNRWALDK
                     AEEKIKFLKSDPAVSQLEAVKKGHIVVMDGQAMNPTIRTLYGAEQVGEQLRKMGLN"
     gene            1307..2344
                     /locus_tag="ACHKFK_004449"
     CDS             1307..2344
                     /locus_tag="ACHKFK_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HFO9054651.1"
                     /translation="MSVAAIETRRSILLTGWCVLAAIVLALVIAVGVSVGELAIPLQN
                     VFYAISNRTGLTAEPLNRIYESVIWDFRLSRALVAACCGAGLAICGVVLQSLLKNALA
                     EPYVLGVSAGASTGAVSIVVLGLGAGAISLSAGAFAGAFAAFAFVALLTNGARGGNER
                     TILAGVAASQLFNAITAYTISTSASAQQARDVMFWLLGSFSGVRWPEFQLVIVVVLAG
                     LAVCLWYARALDAFTFGDDAAASLGIAVPRVRLILFTTAALITATIVSMAGSIGFVGL
                     VVPHVMRFFFGPLHRTLLIASALAGAILMVLADIASRLLIAPQSLPVGVVTALVGVPF
                     FAVIIYRSRNK"
     gene            2344..3105
                     /locus_tag="ACHKFK_004450"
     CDS             2344..3105
                     /locus_tag="ACHKFK_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9054652.1"
                     /translation="MSICAENITWKAGKKVIVNNVSLRVPRGETVGLLGPNGCGKSSL
                     LRVLAGLRRPDAGRVTLDGQDIAQMAKKQLARRVAYVEQHGMTEANMRVRDVVRLGRI
                     PHHSPFSNWSAQDDEAIAAALQRVAMLEKSEQGWLSLSGGERQRVHIARALAQSPSEI
                     LLDEPTNHLDIHHQMQLMQLISELPVTSIVAIHDLNHAAMFCDSLIVMQQGQILASGT
                     PEEILSEALLWDVFRVKTKIEISPYHGKKHIHFIV"
     gene            3162..4349
                     /locus_tag="ACHKFK_004451"
     CDS             3162..4349
                     /locus_tag="ACHKFK_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HFO9054653.1"
                     /translation="MLLGSQFVFNIGFYAVVPFLALFLRDDMLLSGGLIGLILGLRTF
                     SQQGMFILGGTLADRYGAKAIILAGCVVRVAGFLLLACGASLWPIILGACLTGVGGAL
                     FSPSIEALLARAGTHSQANGKRSRAEWFALFAVCGELGAVIGPVAGGVLSGIGFRHIA
                     LAGAGIFLLALAVLFFGLPADGHTTTTRRRVPWWTPLRQPRFVAFILAYSSWLLSYNQ
                     LYLALPVEIQRSGGREQDLAPLFMLASLLIITLQLPLARFARRMGAVRILPVGFLLLS
                     ASFASVALFAAAPPAESWLRLMPAAGFVTLLTLGQMLLVPAAKDLIPLFAEESTLGAH
                     YGALATAGGCAVLAGNLLLGHLLDQALIPSPQAVYPWLLLALFPLCSAVALRAICRPL
                     AAT"
     gene            complement(4427..4702)
                     /locus_tag="ACHKFK_004452"
     CDS             complement(4427..4702)
                     /locus_tag="ACHKFK_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO9054654.1"
                     /translation="MKYSKPISSPSCFGERISTELEFVRKICRAEGETEADDDKLVDE
                     FRRLAEHPDGSDLIYYPRDDREDSHEGIVKEVKEWRAKNGKPGFKQG"
     gene            complement(5060..5359)
                     /locus_tag="ACHKFK_004453"
     CDS             complement(5060..5359)
                     /locus_tag="ACHKFK_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9054655.1"
                     /translation="MSYEIEWRPGAIEDITALFEYLAENASLWDARNVTERLITATDR
                     LADYPRLYETDERYGEGVRRISLLGQHVLYEVNDKARRVNILAVVGQRQNPRFIL"
     gene            complement(5349..5612)
                     /locus_tag="ACHKFK_004454"
     CDS             complement(5349..5612)
                     /locus_tag="ACHKFK_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HFO9054656.1"
                     /translation="MNSTTHYENANFLRELAESLPRIFPEGSTDKSALLQRLANEELA
                     RAEYDEQIRAKVAAARADKRPGMSSVQLRQQLQGRYQELRNEL"
     gene            complement(5632..5769)
                     /locus_tag="ACHKFK_004455"
                     /pseudo
     CDS             complement(5632..5769)
                     /locus_tag="ACHKFK_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077272.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            complement(6189..6647)
                     /locus_tag="ACHKFK_004456"
     CDS             complement(6189..6647)
                     /locus_tag="ACHKFK_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054657.1"
                     /translation="MRLFLGGRCYTAEKLEKDYLAEVANYSNDRWEAPQRASRLAASV
                     KRYKTSEMLRFIFATIAYDPDPDLTPLTVRRLCKALFGRTGSQWLVVEVFGEKGRQHR
                     SADSNPEMVEKMAARYRHAAELHWSATLAEIERVKRLYQMKIKKSKKEVG"
     gene            complement(6788..7636)
                     /locus_tag="ACHKFK_004457"
     CDS             complement(6788..7636)
                     /locus_tag="ACHKFK_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFO9054658.1"
                     /translation="MNNDELATRRAQAIAEDRCFSKGRLRDEFRMKPAPGAEPVKWYK
                     NTYGGRFAVYRIADCVPMREKRPLTSKQQLAGQRLSVLSRLNSTSGRMARQAYDWLSL
                     APLFLDTETTGLDNTAEALEIGLTDAAGQVVFETRLKPMVAIGAQAAAVHGISEQALC
                     GAPSWTDVARQLRHAIGDRPVIIFNARFDIRILKQTAAAHSDPADWLEELTVYCAMEL
                     AAGYYGATNRYGTISLACAASQAGLTWEGQAHSAIADARMTAGVVNAIAAYHLELLQE
                     QARLEI"
BASE COUNT         1536 a         2226 c         2111 g         1834 t
ORIGIN      
        1 tccataaggc attctttgag gcaaagcagc gatacggtgc tccccgcctt gcataatctt
       61 gcagtggcga tcgaacatta caatgaaaac catccgcaca gtgcgttggg ttattgctct
      121 ccgcgagaat atcgacgtca gtgggtaacg ttaacttaag atacaacccc tgtctggaaa
      181 taagggggca agaacacatt gacactgtaa atatggcatt gtataaaacc ccgccccaat
      241 aatgagtttc atttgcatta ctcccatttg ctggtcatcc actcgcctgt ttacaaggat
      301 agcgtttaat gaagaaggtc ctttgcgcgt taggcttgat gttcaccgcc gtcagttccg
      361 cgctggcaac aacctacccc ctgacgattg aaaactgcgg ctatcaggag accttcaccc
      421 ggccgccgga gcgcgtggtc gcgctggggc aaaatacggt cgaaattctg ctgctgctcg
      481 ggctgcaaaa gcaggtggtc gccagcgcct tctggccgac cagcgtttta ccgcagctgg
      541 ctgagcaaaa cgcgaagatt aaaaccttga cggtggaaat tccgagcctg gaatcggtgc
      601 tggcgcaaaa tcctgatttc gttcccgccc agttgccgct gctgctgggg ccggaaagca
      661 aagtcgccag gcgtgaagat ctcgccaccg tcggcgtgaa cagctacgtc tcaccgggga
      721 tgtgcgccac caaaaaggcc accggcgata tgtacggcag ccggcaaaag ctgtgggata
      781 tgacctggct gtatcaggaa attagcgatt tttcccgcat tttcaacatc gaagatcgtg
      841 gtcaggcgct gattgccgat ttcaaaaaac gtgaagccga cctgcgccag gagtttggta
      901 aaagcaaaaa ggatctctcc ttcgtcttct ggttctccag cgcctcacct tcggccgatg
      961 cctatgtcgg cggcaaaaac agcgcctccg gatttatcgc cagcgtgctg ggcggacata
     1021 atgcgattac ctccgaaacc gagtggccca cggtgagctg ggagagcatt attgccgcca
     1081 acccggatgt gatcgtggtc gccagcctgg atcgtaaccg ctgggcgctg gataaggccg
     1141 aggagaagat caaattcctc aaaagcgatc ccgccgtcag ccagctggag gcggtgaaaa
     1201 aaggccatat cgtggtcatg gacggtcagg cgatgaatcc gaccattcgc acgctttacg
     1261 gcgcggagca ggtgggcgag cagctgagaa aaatggggct gaactgatga gcgtcgccgc
     1321 gattgagacg cgccgcagca tactgctcac cggctggtgt gtactggcag cgatcgtgct
     1381 ggcgctggtg atcgctgtgg gcgtcagcgt tggcgagctg gccatcccgc tgcagaatgt
     1441 cttttatgct atcagcaaca gaaccggctt aaccgctgag ccgctcaacc gtatctatga
     1501 gagcgttatc tgggatttcc gcctcagccg cgcgctggtc gccgcctgct gcggcgccgg
     1561 cctcgctatc tgcggtgtcg tgctgcaaag cttgctgaag aatgccctcg ccgaacccta
     1621 cgtcctcggc gtctccgccg gggcctccac cggcgcggtg tcgatcgtgg tgctgggcct
     1681 tggcgctggc gcaatttccc tctcggcagg cgcctttgcc ggggccttcg ccgccttcgc
     1741 ctttgtcgcc ttgctgacta acggcgcgcg cggcggcaat gagcgtacga ttcttgcggg
     1801 cgtcgccgcc tcgcagttat ttaacgccat cacggcctat accatcagca cctccgccag
     1861 cgcacagcag gcgcgcgatg tgatgttctg gctgctgggc agcttcagcg gcgtccgctg
     1921 gcccgaattc cagttggtca tcgtggtggt gctcgcgggt ctggcggtct gcctgtggta
     1981 tgcccgggcg ctggacgcgt tcaccttcgg cgacgatgcc gccgcctcgc tggggattgc
     2041 tgtgccgcgc gtgcgcctga tcctcttcac cactgcggcg ctgatcaccg ccaccatcgt
     2101 cagcatggcc ggctcaatcg gctttgttgg cctggtggtg ccgcacgtga tgcgtttctt
     2161 ctttggccca ctgcaccgaa ctttgctgat cgccagcgcg ctggcagggg cgatattgat
     2221 ggtactggcg gacattgcgt cgcgcctgct gattgccccg caaagtctgc ccgttggggt
     2281 agtaaccgcc ctggttgggg tgcctttctt tgccgtgatt atctaccgct caaggaataa
     2341 gtgatgagta tttgcgctga aaatatcacc tggaaggcag gcaaaaaggt catcgtcaat
     2401 aatgtctcgc tgcgggtgcc gcgcggcgaa acggtcggac tgctggggcc caacggctgc
     2461 ggcaaatcct cgctgctgcg cgttctggcg ggcctgcgcc gcccggatgc aggtcgcgtc
     2521 accctcgacg gccaggatat cgcccagatg gcgaaaaagc agctcgcccg ccgcgtggct
     2581 tacgtcgagc aacacggcat gaccgaggcc aatatgcggg tgcgcgacgt cgtgcgcctg
     2641 ggacgcattc cccaccactc tccgttctca aactggagcg ctcaggatga cgaagcgatt
     2701 gccgccgcgc tgcagcgggt agcgatgctg gagaaaagcg aacagggatg gttaagcctc
     2761 tccggcggcg agcggcagcg ggtgcatatc gcccgcgcgc tggcgcagag cccgagcgaa
     2821 atcctgctgg atgagccgac caaccatctg gatatacacc atcagatgca gttaatgcag
     2881 ttgatcagcg agctgccggt aaccagcatt gtggccattc acgatcttaa ccatgccgcg
     2941 atgttctgcg attcgctgat cgtgatgcag caggggcaga tcctcgccag cgggacgccg
     3001 gaggagattt tgtccgaagc gctgctgtgg gacgttttcc gggtgaaaac caaaatcgag
     3061 atctcccctt accacggcaa aaagcacatt catttcatcg tttaggttgg gcggatatgc
     3121 ctcttttctc cctgcgccct gcggcaaccc tctggcctcc ggtgttactg ggaagccagt
     3181 tcgtttttaa tattggcttt tacgcggtcg ttcctttcct cgccctcttc ctgcgcgacg
     3241 acatgctgct ctcaggcgga cttatcggcc tgatcctcgg gctgcgtacc ttctcccaac
     3301 aggggatgtt tatcctcggc gggacgctgg ccgaccgcta tggcgctaag gcgatcattc
     3361 tcgccggctg cgtcgttcgc gtcgccggtt ttctcctgct ggcgtgcggg gcgtcgctgt
     3421 ggcccattat ccttggcgcc tgcctgaccg gcgtcggcgg cgccctgttc tccccgtcga
     3481 tagaggccct gctggcgcgg gccggtaccc acagccaggc caacgggaaa cgcagccgcg
     3541 ccgagtggtt tgcgctgttt gcggtatgcg gtgaactggg agcggtgatc ggcccggtgg
     3601 ccggaggcgt gctgagcggg atcggctttc ggcatatcgc cctcgccggc gcgggaatat
     3661 ttcttctggc gctggcggtg ctctttttcg gtctgcccgc cgacgggcac acgacaacaa
     3721 cgcgcagacg agtcccatgg tggacgcctt tgcgccagcc gcgctttgtc gcgtttattc
     3781 tcgcctacag ctcatggctg ttgagctaca accaactgta cctcgccctg ccggtggaga
     3841 tccaacgttc cggcggccgc gagcaggatc tggcgccgct ctttatgctg gcctcgctgt
     3901 tgattatcac cctccagctc cctctcgccc gcttcgcccg gcgaatgggc gccgtgcgca
     3961 tcctgccggt gggctttctg ctgctgtcgg cttcctttgc cagcgtggcg ttattcgccg
     4021 ccgccccacc cgccgaaagt tggctgcggt taatgccggc ggctggtttt gtcacgctgc
     4081 tcaccctcgg ccagatgctg ttagtgcccg cagccaagga tctgatcccg ctgttcgccg
     4141 aggagtcgac gctgggtgcc cactacggag cgctcgccac tgcaggaggg tgcgcggtgt
     4201 tagccgggaa tttactgctc ggtcatttac ttgaccaggc gctcattccg tcgccgcagg
     4261 cggtttatcc ctggcttctg ctggcgctgt ttccgctgtg cagcgccgtc gccctgcggg
     4321 cgatctgtcg cccgctggcg gcaacatgac gcagagcggg taacagcccg ctctggaagt
     4381 gaacagctag tatccatatt ttcagctaat actttagcaa agatcctcag ccctgtttaa
     4441 accccggctt gccgtttttg gctcgccatt ccttcacttc ttttacgata ccttcatgac
     4501 tgtcctcacg gtcatccctt ggataataaa taagatcaga accatcaggg tgttcagcta
     4561 atcgacgaaa ttcatccact aatttatcgt catcagcctc agtttcacct tcagcccggc
     4621 agatttttct gacgaactcc agttcagttg atattctctc gccaaagcag ctggggctgg
     4681 atataggttt ggagtatttc aaatacctgg aagatcaatt ttgtgctgca gcttttaatt
     4741 ttctacgcaa tgctgatatg actcaaaacg atgtgaaaga tacagagtca gcttatcgtg
     4801 gatggctgga gattgacgaa gaagatcttg agtaaagctc gataattctt ctgacgttat
     4861 aactaacgga tgtcgcgtaa gcgagccttc agggccgctt acgagcgagt atgacagacg
     4921 tatagggttt cttcatgttc tcagtttttg aattgtcaat tctactagtt caactcaata
     4981 ctcttagcat gggaaaattt catttctcca ggcggctccc aaaccgtgat tccaggtaag
     5041 cccgcatcaa gccgggcact caaagaatga atcggggatt ctgacgctgg cctacaaccg
     5101 ccaggatatt gacacgacgg gccttatcat tcacttcata aagtacatgc tgcccaagta
     5161 agctaatacg gcgaacccct tcgccatagc gttcatctgt ttcatacagc ctggggtaat
     5221 ctgccagacg atcggttgca gtgatcagac gctctgtcac gttccttgca tcccaaagag
     5281 atgcattctc ggccagatat tcaaacaacg ccgttatatc ctcaatcgca cctggtcgcc
     5341 attctatttc ataactcatt gcgcagttcc tgataacgtc cctgtagttg ctggcgcagt
     5401 tgcacggaac tcattcccgg ccgcttatcg gcgcgagcag cggcaacttt cgctcggatc
     5461 tgttcgtcgt attccgcccg tgccagttct tcattagcga ggcgttgcaa tagtgcagac
     5521 ttgtctgtac tgccttctgg aaaaatacga ggcaggcttt cggccagctc gcgcaggaaa
     5581 ttggcgtttt cgtaatgtgt ggtgctattc ataattgact ccccctttca ttcaataaag
     5641 ggcaattgca ggttttttga tgccgcacgc atgggtataa cgagtcagag tatcgatatt
     5701 cgccccggtg atattgtttc acaggcaggc gataaccgga tgcataatgc tcatttgcct
     5761 ggcaatctgc gtgttgtcag gttttatgca gagtatataa cctttcagac acagccacca
     5821 acatatgctt atgccgcgcc agcggcatgt aaggcggcac gctgtggtta catgtgatac
     5881 cggagtaaaa ccgacgaagc ccggcgtaag ccggtactga ttgatagatt tcaccttacc
     5941 catccccagc cctgccagac catacccgct ttcagccatg atagagcctc tgtgcgcggt
     6001 cggagtggtc ccgacgaggg tttgaagtcg gggcgtatct ccgcgttagc gggccgtcag
     6061 ggccgcttac gagcgtgtat gagatcagtt tggatattag aaataattgt acctttaacg
     6121 caccatcatt aatgctaatg tatgcataca ttagcattaa tgatgaagga gaagaataac
     6181 aatggcagtc aacccacttc ttttttggat ttcttaattt tcatctgata aagccgcttt
     6241 acccgttcaa tttctgccag cgttgcggac cagtgtagtt cagcggcatg acgatatctg
     6301 gcagccattt tttccaccat ctccggattg ctgtccgcgc tgcggtgctg acgccccttc
     6361 tccccaaaaa cttcaacaac cagccactgg cttccggttc tgccgaacaa tgccttgcac
     6421 aaacgtctca cagtcagcgg ggttaaatct ggatcgggat cgtaggcaat ggtagcaaaa
     6481 ataaagcgca gcatttctga cgtcttgtaa cgctttacgc tggcagccag gcgtgaggca
     6541 cgttgcggtg cttcccatcg gtcgttactg taattagcca cttcagccag ataatccttt
     6601 tcaagttttt cagccgtgta gcacctgccg cctaaaaaaa gccgcatggc ctccctggtt
     6661 ttgtctctca catatcatcc ctcattaatg ctaatgtatg catacattag cattaacatg
     6721 ctgacagcca caagcggcgg atttacgtcc ggataacgta aatgattgcg gtatacaggc
     6781 caggcggtca gatttccagc cgtgcctgtt cctgcagcag ttccagatga tatgcagcaa
     6841 tagcgtttac cacccctgcc gtcatccgtg cgtcggcgat cgctgaatgt gcctgccctt
     6901 cccaggtcag tccggcctgg ctggcagcac aggccagtga aatcgtgcca tagcggttgg
     6961 tggccccata atatcctgca gccagctcca tcgcacaata taccgtcagt tcttccagcc
     7021 agtcagccgg atcgctatgt gcggcagcag tctgtttcag aatgcggatg tcgaaccggg
     7081 cattaaagat aattactggt cggtccccga ttgcatgccg cagttgacgc gccacatctg
     7141 tccatgatgg tgcaccgcag agagcctgct cactgatacc atgcacggcg gcagcctgcg
     7201 ccccgatggc gaccatgggc ttcagcctgg tttcaaatac cacctgaccg gccgcatcgg
     7261 tcaggccgat ttccagggct tccgcggtat tatccagtcc tgtcgtttca gtatccagaa
     7321 agagtggtgc cagactcagc cagtcatatg cctgccgtgc catccggcca ctggtgctgt
     7381 ttagtcggga taaaacggaa agccgctggc cagccagctg ttgtttactc gtcagagggc
     7441 gtttctcacg catcggaaca cagtctgcaa ttcggtatac agcgaacctg ccaccataag
     7501 tgtttttata ccacttaacc ggctcagcac cgggggctgg tttcattctg aattcatcgc
     7561 gcagacgtcc cttcgagaaa cacctgtctt cggcaatcgc ctgcgcacgt ctggtggcca
     7621 gctcatcatt attcatggtt attgcatctc cttatatccg gacagcccct ctatggccac
     7681 ttctgtgcag tatgcaatac acgcagg
//
LOCUS       DAZGJT010000164         7667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZGJT010000164 DAZGJT010000000
VERSION     DAZGJT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.167"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            337..588
                     /locus_tag="ACHKFK_004458"
     CDS             337..588
                     /locus_tag="ACHKFK_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO9054659.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSSGDGYPVAILNRNQPAFYC
                     VPAELYERMLDALDDQELVKLVTERSNQPLHDADLDSYL"
     gene            585..872
                     /locus_tag="ACHKFK_004459"
     CDS             585..872
                     /locus_tag="ACHKFK_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9054660.1"
                     /translation="MTYTVKFRDDALKEWLKLDKTIQQQFVKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVIDDMLIIAVVAVGKRECSNVYNLASERMR"
     gene            complement(1008..2221)
                     /locus_tag="ACHKFK_004460"
     CDS             complement(join(1008..1930,1930..2221))
                     /locus_tag="ACHKFK_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9054661.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQSEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENLELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWRGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTPPAEAEKA
                     YYASIGNDDLAA"
     misc_feature    complement(1824..1940)
                     /locus_tag="ACHKFK_004460"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(2445..3146)
                     /locus_tag="ACHKFK_004461"
     CDS             complement(2445..3146)
                     /locus_tag="ACHKFK_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFO9054662.1"
                     /translation="MTMTISTAKKTVNISQSNELTEAAYYLPLQAKRVLWLCLIQCYP
                     QKDDPDSVSPVFTVLVADYQKYFKVSVDTASADVKKGVTALAESSVVFYPKDCEFDEI
                     KRPWLAEAGLKKGRGKWQIEFNYKVMPYLMGLSSQFTTYSLYDCGKINSVRVIRLYES
                     LCQYRSSGVWVTTQEWLSERFMLPASQRSNFAEMKRTFINPALKKINTNTPLKATMIQ
                     NENGSLVFTIVDSKK"
     gene            3523..3726
                     /locus_tag="ACHKFK_004462"
     CDS             3523..3726
                     /locus_tag="ACHKFK_004462"
                     /inference="COORDINATES: protein motif:HMM:NF020355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO9054663.1"
                     /translation="MRIITIPLLCRYASSPVRSRCSVTRLRRAFQTAAVASEQISVFP
                     VINDISLKDYQLLLKVAEEVDKK"
     gene            3923..4075
                     /locus_tag="ACHKFK_004463"
                     /pseudo
     CDS             3923..4075
                     /locus_tag="ACHKFK_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            4178..4738
                     /gene="istB"
                     /locus_tag="ACHKFK_004464"
                     /pseudo
     CDS             4178..4738
                     /gene="istB"
                     /locus_tag="ACHKFK_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
     gene            4811..5038
                     /locus_tag="ACHKFK_004465"
                     /pseudo
     CDS             4811..5038
                     /locus_tag="ACHKFK_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5824..6258
                     /gene="psiB"
                     /locus_tag="ACHKFK_004466"
     CDS             5824..6258
                     /gene="psiB"
                     /locus_tag="ACHKFK_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO9054664.1"
                     /translation="MNTELNLNVLQTMNALEYEDIRAAGSDERRELTYAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWMLVLVNSGGEPFSMV
                     QVHRLFTPVAVSHSLALAASLDERGYSVSDIIHILMAEGGQS"
     gene            complement(6357..6510)
                     /locus_tag="ACHKFK_004467"
                     /pseudo
     CDS             complement(6357..6510)
                     /locus_tag="ACHKFK_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011149570.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            6585..7272
                     /locus_tag="ACHKFK_004468"
                     /pseudo
     CDS             6585..7272
                     /locus_tag="ACHKFK_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         2080 a         1778 c         1865 g         1944 t
ORIGIN      
        1 aacgcggaga tacgccccga cttcgggtaa accctcgtcg ggaccactcc gaccgcgcac
       61 agaagctctc tcatggctga aaacgggtat ggtctagcag ggcaagggat gggtaaggtg
      121 aaatctatca atcagtaccg gctgacgccg agcttcggcg gttttactcc tgcgccatat
      181 gctacaacgg agttccgcct tccatgccgc tgacgcggca tcagtgcaat acaagcaaca
      241 ttttgtgtat tttcaaaagc acgagtaata aagacttttt aagtggtcat ttcagaggta
      301 tctttaagac ttgttaaaca gtctcaaagg tacgttatgc ctaacatcat cctgtctgac
      361 accagtgcaa gtgtcagtga actgaaaaaa aatcctatgg ctacagtcag ttccggagat
      421 ggttaccctg tcgccattct taaccggaat cagccggcat tctactgtgt gcctgctgaa
      481 ctgtatgaaa ggatgcttga tgctctggat gaccaggaac tggtgaaact ggttactgaa
      541 cgcagcaatc agccactgca tgatgcggac ctggacagtt atttatgact tatacggtaa
      601 aattcaggga tgatgcgctc aaagaatggc tgaaactgga taagaccatt caacaacagt
      661 ttgtgaaaaa actaaaaaaa tgcagcgaaa atcctcatat accgtcggca aaactacgtg
      721 ggctaaagga ctgctacaaa attaaacttc gtgcatcggg atttcgtctt gtttatcagg
      781 tgattgacga tatgttaatt attgcggttg ttgcggtagg taaacgagaa tgtagtaacg
      841 tttataatct cgccagtgag agaatgagat aggaacatat aaatagcctt tcccgcagat
      901 aactctcaga attgttatat tctctgtgga ctcgcctgag gcaggaaaag aaaagttttg
      961 ttgagtgaac cgccccgggt ttcctggaga gtgttttatc tgtgaactca ggctgccaga
     1021 tcatcgtttc cgatggaagc ataataagct ttttctgctt ctgccggagg agtatggccc
     1081 agcctttcca gcaatcgtcg attgttatac cagtccaccc acgtgagtgt ggccagttcc
     1141 acttctgcac ggtttttcca gctcttacgg tgtattacct ccgctttgta aagaccattg
     1201 atgctctccg ccatcgcgtt gtcatacgag tcgcctgtac ttcctgttga tgccagtaat
     1261 ccggcttcct taagccgctg tgtgtaggcc agcgatacat actgagaacc tttatcactg
     1321 tgatggaccg tgccggacgg tcgacgggcc cataacgcct gctccagtgc atccagcacg
     1381 aatgtcgttt ccatggacga tgagacccgc caccccacga tgtatccggc aaacacatca
     1441 atgatgaacg ccacatagac gaagccccgc catgtgctga cgtaagtaaa atcagccacc
     1501 cacagctggt caggtcgttc tgccacgaac tgacggttta cgcggtcgcc tgcggcaacg
     1561 gctttccggc tgatggtcgt acggaccttt ttaccccgga gaacaccggc aagtcccata
     1621 accgccatga gacgtgccac agtgcatctg gccactctga taccttcccg taacaactga
     1681 cgccagactt tacgcacacc gtataccttg tgattttcat cgtatacgcg ctgtatctct
     1741 ttcttcagcc agtcatcgcg ctgcgcacgg gcactgcgtt tatccggatg atgtcgctgt
     1801 tgctgacagt ggtaatacgt tgacggggca atatgcagtt cgctgcatag cggtccgacc
     1861 ccgtactgct cacgcagctt atccagcagt ggcatcattt tttccagagg cggtcgaact
     1921 ccgccttcgc aaaataagcg gaagcctggc gaaggatatc gttactgcgg cgcagttcaa
     1981 gattttcacg ttccagctct ttcagacgct gacgttcagc ggtggtgagc cctccatcac
     2041 cgcctccggt atcccgctca tgctggcgaa cccagacacg cagagtctcc ggcgtacagc
     2101 caatctttgg agcaatggaa caaattgtcg cccattgtga gtcatattcg ctctgacttt
     2161 ccagaaccat acgaatcgcc ctctgacgga cttcggggga aaaacgagta tttttagtca
     2221 tcctgtttac ctctttctca gggagtttag tctccaggat tcccggggcg gttcagttac
     2281 ttgatggaaa gcgcatctgg gtcgagaaaa atttaagcca gatgtaatct gacagatacc
     2341 tgtataaata accggtaact gtcagatcag gtctgagcta atacagatta tttcactatg
     2401 ggaaatagcc ctagtttacc ttcgtcgtat tgtatacagt ttggttactt tttggaatca
     2461 acaatggtga agaccaagct accattttca ttctgtatca ttgtcgcttt gagtggtgta
     2521 tttgtattta tctttttgag tgccggattg atgaacgttc gcttcatttc agcgaagttt
     2581 gatctctgtg atgcaggaag cataaagcgt tctgacagcc attcctgggt tgtaacccat
     2641 actccgctgg aacggtattg acataagctt tcgtacaatc tgatgaccct gacactgttg
     2701 attttcccgc aatcatagag tgagtatgtt gtaaactgac tggacaatcc catcaaataa
     2761 ggcattactt tgtagttaaa ttcaatttgc catttccctc gtcccttttt tagaccagct
     2821 tcggccagcc agggacgctt aatctcgtca aactcacaat cctttggata aaagacaaca
     2881 ctgctttcag ccagtgctgt aacgcctttt ttaacatcgg ctgacgctgt atcaacagac
     2941 actttgaaat acttttgata atcagcaaca agaactgtga ataccggaga taccgaatcg
     3001 ggatcatctt tctgtggata gcactggata agacatagcc ataatactct cttggcttgc
     3061 aacggcaaat aatatgccgc ttccgtcagt tcgttggatt gactaatgtt aactgttttt
     3121 ttcgcggtac ttattgtcat tgtcattagt atataggttt ttttaattcc tatattgtca
     3181 tgcagatgta aacctaacaa gcgaaattaa tgagttatcc acagttatag catatgcatt
     3241 taaactaggg gcatttccca caggaactgg tgctatttcc catagtaact tgttgtatta
     3301 cccataataa ctatatccat ttcccatagt aacttgaggt catccccata gaaggaaatc
     3361 tgaaatacaa gtgcgacagg gactcagagg ctcctaaaaa ggataaaaag taaaaaaata
     3421 taaaatgttt aataaatagt ttgtggataa gtggataagt ggataagttg acctgaattg
     3481 tcattaaact taggttattt cccataatat cttttgaatc agatgcgtat aatcactatt
     3541 ccactattgt gccgttacgc ttcctcgcct gttcgctcgc gatgctcggt cacaaggctg
     3601 cggcgagcgt ttcagacagc tgctgtagca agtgaacaga tttcagtatt tcctgtcatc
     3661 aatgatatct cattaaagga ttaccaatta ttactgaaag ttgccgaaga agtagataag
     3721 aaataagtgt ctttgaaaga attgatggaa aaaatttgaa atcagtgagc aaatgccaag
     3781 ttagccaagc catgctgata tattaactaa gtatagtctt gttgtttttc ctctacgcac
     3841 tgcaaccttg tggctgtact atattcgaat gctgtcgcac cgttacaagt gtccatgtct
     3901 gaaaactgac gtgtcaagca gacagtacag cgataaatgg atgaccgaat ccaggtagga
     3961 aaagctgata tggctgtgtg cacagatgaa gctgacaaga cagtgctggg cgctgaaaga
     4021 gctggcaaag gatatctgga acaggccatg gagtgaggaa agacgtaatg actgggagag
     4081 attgtcagcg ccagtgatat aagacggtaa ttcaccattt ggattgtccg ctccacccaa
     4141 catgttgttt ccttaaggtt ctcacaccag aaaggacatc gaccgtaagg ttgttcggga
     4201 actggctggt ctggcgttcg tggagcgcag cgaaaacgtg atcctgctgg gacctcctag
     4261 tgtcggaaaa acgcatctgg ccatagctct tggcgtgaaa gcggtggatg cgggacatcg
     4321 ggtactgttt atgccattgg acagactgat cgcgacactg atgaaagcga aacaggaaaa
     4381 ccggctggag cgtcagctgc agcaactgag ttatgcccgg gtgttgatcc tggatgaaat
     4441 aggctatctg ccgatgaaca gagaggaagc cagcctgttc ttccggctac tgaaccgtcg
     4501 atatgaaaaa gcgagcatca tactgacgtc aaacaaaggg ttcgcagact ggggagaaat
     4561 gttcggagat cacgtgctgg caacagcgat actggatcgg ttgctacatc actcaaccac
     4621 gctgaatatc aaaggagaga gttaccggtt aaaagagaaa cgtaaagctg gagtgctgac
     4681 caaaaacaca acgccaatca gtgatgatga aatggtgaaa agcggacagc atcagtaacg
     4741 aaagtatctt agcgggcatg aaaatggcaa ataacagtca aacatcgtgg cgttgacaag
     4801 atgacaggtt ctggcggcca acagtgacgt tcctatgatg aaaaacgccg cgaaaacgat
     4861 aggaaaaagg ctttacggga tcctgaatgc catgcgacac cgtgcctcaa acggaaacgc
     4921 ggaggcgctt aacagtaaga tcaggctact gaggataaaa gccaagggat accgcaatcg
     4981 ggagcgcttt aagctgggcg tgatgttcca ctacgggaaa ctgaatatgg cgttctgagc
     5041 cttcccacca tgatcaggga agacccatct ataaacagca ctcccgttat ttttctgaaa
     5101 tcattgttaa aactataggc actatgctga caggatcgcc tccccgtcat atacgaggca
     5161 tcattgggtg acaatgagcg aatattcaga attattccgg aagcatgctg aaactgtcag
     5221 tgcacaatgt cggacgggga agcgtccgac agacattaac tgcacgatga ggagatagtc
     5281 atgtctttag aagagactgt accaaatcct ccctaaatct gcaaaatgta gaaaatcgtg
     5341 ccgtctgccc tagagtagct gacaggtctg tacgcttaaa aagcaattcc gcaggaatta
     5401 aaaacggtca ccatcggaat acgactccta cgaagtagat tgttggagtt cgtgcaataa
     5461 cgcagtaaag aaaatatccg agcaaattcg cgacgtgctg gcagaagaca gaatcacaac
     5521 tgaactctat cgccggagaa cgacatggag cgtcatcttt atgtgtttga agtggcctgt
     5581 caggatggag ttacaaacta gaagtacaga gcattcgtca ctaggtgacc gaaattgaag
     5641 tggttgtatc cagtgccgca caggatatga agaaaggtga tacagctaaa tatgcagtgc
     5701 actacgcgtc cacagacaga gactaacccc agtgaataca cagacaatcc ggctgatgcc
     5761 gacttgatga acaatcacac cgccccgccg gagacggggc gccaataagg aatatatatc
     5821 acaatgaaca ccgaactgaa cctgaatgtc ctgcagacta tgaacgcact ggaatatgaa
     5881 gatatccggg ctgccggaag tgatgaacgc cgtgagctga catacgccgt gatgcgggag
     5941 ctggatgcgc cggacaactg gacgatgaac ggagagtatg gcagtgagtt tggcggcttt
     6001 ttccccgtcc aggtccgttt cacacctgcc cacgaacgtt ttcacctggc gttatgttcg
     6061 ccgggcgatg tctctcaggt ctggatgctg gttctggtga attccggtgg tgagccgttt
     6121 tcgatggttc aggtgcatcg tctgtttaca ccggtagctg tcagtcactc actggctctt
     6181 gcggcgtcac tggatgaacg ggggtattcc gtcagtgaca tcatccatat cctgatggcg
     6241 gaaggaggtc agtcatgaga gcacgttcac agacattgcc cccctctgca cagagcaaca
     6301 gaccgcatgg tgggtatgac tgagacggaa aaaggccgct ccgtgcgcaa taccgttcac
     6361 ttaagcggag cggcactgcg tttcacagga taacggtttt tttgatatct taaccgccct
     6421 cggccttgat ggtcggaggc gttttgttat gaacaccact tccagagcat cccgcagacg
     6481 ctccggtcgt ttcgggatgg ttcccggtga tgccgtgttt cccagcacta tgaaccgccc
     6541 cgggaatcct ggagactaaa ctccctgaga aagaggtaaa caggatgact aaaaatactc
     6601 gtttttcccc cgaagtccgt cagagggcga ttcgtatggt tctggaaagt cagggcgaat
     6661 atgactcaca gtgggcggca atttgttcca ttgccccaaa gattggctgt acaccggaga
     6721 ctctgcgtgt ctgcatcgtt gaagcaccga cgccgtcccg ccgcgataga gcgtggtatc
     6781 gcgggccccg gattacttgc ccgcgtgtta acgggaaaat actgcgaata cctgccactg
     6841 tatcgtcaga gtgaaatctt tgcccgacag ggtgtcgaac tgagccgtgc attactctcc
     6901 aactgggttg atgcgtgctg ccagttaatg atgccgctga atgatgccct gtaccgtcat
     6961 gtgatgaata cccgcgagct tcacactgac tacacaccgg taaaggtact ggcaccgggc
     7021 ctgaaaaaga cgaaaacagg gcgcatctgg acgtatgtcc gggatgatca caatgcgggt
     7081 tcgtcatctc ctccggcggt ctggttcgcg tactcaccga accggcaggg gaaacccccg
     7141 gggcaacacc tccgcccctt ccggggtatc ctgcaggcgg atgcgttcac cggttacgac
     7201 aggctgttca gtgcagaacg tgaaggtggt gcgctgacag aagttgcgtg ctggactcat
     7261 gcccggagaa agtttgctga gtcacacata taactgcttt tcattcacgg gttgcggcag
     7321 cgacacggat gcattccgca agcttcagag cttcactgag aaatataggc gaatcataaa
     7381 ttaccaatac tggcttaacg gacattttgc gtaggtttat tcacatatac acattttttg
     7441 acaaaaaaac atatttgctc actatttttg cacttaattt tcattttatt caagtatgat
     7501 agtagcaaaa aagtatcaga tcattgtttt taatataagc taatctacca aaaaggataa
     7561 aataattttt agttatttag catattataa atttgaacgt gagaacttgg taaaaacaac
     7621 gcagattaag tcgtattatc ttccatagca aggtcatgga gaactat
//
LOCUS       DAZGJT010000165         7531 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZGJT010000165 DAZGJT010000000
VERSION     DAZGJT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.168"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..173
                     /locus_tag="ACHKFK_004469"
     CDS             <1..173
                     /locus_tag="ACHKFK_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO9054665.1"
                     /translation="EFTLRLSILTSEQPVLVLRIVRVEAAEEEIAKEFRDLLKERFEE
                     EDILTFIGTFSV"
     gene            250..726
                     /locus_tag="ACHKFK_004470"
     CDS             250..726
                     /locus_tag="ACHKFK_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054666.1"
                     /translation="MQFKDLPEDIQKIAADTLKDHLSVINLTKEPKANLENISRNMRD
                     VFVGMYAYDNEKHEEHIQNCRLNKGQQNIKHNITENDKSINSVERVNNSQKTDPLVFW
                     NIVQAFALSKVYEFNNINHSGIDTHQSSHREGVSMLLSLLREQGECPHSTLNDTEQ"
     gene            883..1008
                     /locus_tag="ACHKFK_004471"
                     /pseudo
     CDS             883..1008
                     /locus_tag="ACHKFK_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Eaa protein"
     gene            1084..1497
                     /locus_tag="ACHKFK_004472"
     CDS             1084..1497
                     /locus_tag="ACHKFK_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001449526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO9054667.1"
                     /translation="MEPGNVPLYIGLPRIEPASQTAKRSFQEWLSEQKEKIDVDCGCV
                     SIETLTHWMKSAYEAGNSPVTPDGWISCSERIPDDKQYVWCWGKSYGWTECDTFEGYY
                     DWSRNKWWAVTDDGEEPASKVTHWMPLPEPPQEVN"
     gene            1497..1782
                     /locus_tag="ACHKFK_004473"
                     /pseudo
     CDS             1497..1782
                     /locus_tag="ACHKFK_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241156.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
     gene            1878..2147
                     /locus_tag="ACHKFK_004474"
                     /pseudo
     CDS             1878..2147
                     /locus_tag="ACHKFK_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724814.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2177..2959
                     /locus_tag="ACHKFK_004475"
     CDS             2177..2959
                     /locus_tag="ACHKFK_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFO9054668.1"
                     /translation="MKSIIKHFECEISQGNIVGLDAAFYQGKPVFLAAPFAKALGYAN
                     TADALKKHCKSLIKLNYRESRELGLGDNPRGIQLIGQGDAFRLIMRSSLPSAERVQDW
                     VCEVVLPALMETGTYSLKQEKSTPSNGLPEYRKAKALKMEMEVISSVLDRLPHLGDKA
                     KQAAYASVINRSAGFNVIPLPVLDEHHYSATEVGKQFGVTANKIGRIANTYMLKTEQY
                     GKWFIDKSPYSDKQVESFRYNNRGVRKIEEILEAENKAEFGT"
     gene            complement(3002..3307)
                     /locus_tag="ACHKFK_004476"
     CDS             complement(3002..3307)
                     /locus_tag="ACHKFK_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001240641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO9054669.1"
                     /translation="MAMFNPPHPGEIIADILEDQNIGIRELARALDVAPSTVQRLVSG
                     NATISPEMAVRLAAVLGGTPSSWIRLQTAWSLEKAEREVDVSHLSTKYRPAEISPHA"
     gene            complement(3307..3585)
                     /locus_tag="ACHKFK_004477"
     CDS             complement(3307..3585)
                     /locus_tag="ACHKFK_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO9054670.1"
                     /translation="MIKSWKHKGLQRFFETGNASGINPNHVKRLRTRLQVIDQAEIID
                     DINLAGYRLHQLQGGRSDTWSVTVSGNWRITFEFRNGDAYILNYEDYH"
     gene            3929..4240
                     /locus_tag="ACHKFK_004478"
                     /pseudo
     CDS             3929..4240
                     /locus_tag="ACHKFK_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232082.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            4509..5177
                     /locus_tag="ACHKFK_004479"
     CDS             4509..5177
                     /locus_tag="ACHKFK_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HFO9054671.1"
                     /translation="MTMQLAVESLVVVTYSNVPVITTGLLAHLYKTEEKHIRQNFKRN
                     ECRFIAGKHFFKVSGCELDSLRTSQRGLQISPKTRSLILWTERGAARHAKMLETDRAW
                     EVFEKLEDCYFSQKIPEQLQLPESTLSINYPLSWFSEHHPYSMMSYVDRKTLNLDVSV
                     LFDMPSPTMRILNELHSKGYNVDAAVAEFNAFKHLTEEMRRTLQDISRLSDRNSRKGF
                     SLSL"
     gene            5228..5659
                     /locus_tag="ACHKFK_004480"
     CDS             5228..5659
                     /locus_tag="ACHKFK_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFO9054672.1"
                     /translation="MKQIAFYRRSGRPGVFRGLKERVTWMIQSRGRPVTGSEIAEKFG
                     VSLCEFNKVARSLTKGSKVVKIKASESFTTDTGIVDRFFSLESNPRRDTPRSRNAVPP
                     FSRRSREHAAKNCREEYEQKAKRRRRLIKAGLYIDEFEKAL"
     gene            5656..6282
                     /locus_tag="ACHKFK_004481"
     CDS             5656..6282
                     /locus_tag="ACHKFK_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MT-A70 family methyltransferase"
                     /protein_id="HFO9054673.1"
                     /translation="MTNKYTLIYAAPPWTFRDKATDGQRGASFKYPVMSLLDICRLPV
                     WELAADNCLLAMWWVPTQPLEALKVVEAWGFRLVTMKGLTWNKCGKRQTDKLVMGMGS
                     TTRANSEDCLFAVKGNLPERINAGIIQSFTAPRLDHSRKPDMAREKLVQLLGDVPRIE
                     LFARHTSHGFDVWGNQCGTPSIEMVPGIVKFLEKTNERKNDVDKGITS"
     gene            6242..6454
                     /locus_tag="ACHKFK_004482"
     CDS             6242..6454
                     /locus_tag="ACHKFK_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO9054674.1"
                     /translation="MSEKTTLTKASPVELRQCLEIANQLARSGIRFVPIPITADAELH
                     LFGEILSRKLDELEKLVEEADTSPIA"
     gene            6490..6867
                     /locus_tag="ACHKFK_004483"
     CDS             6490..6867
                     /locus_tag="ACHKFK_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO9054675.1"
                     /translation="MNAVLTELNKLGKATADSISKGLNIDLNDVIDTLWKLKNQGIVK
                     VKNGIWQAVEGVGSKTDIDSVQPVQPVQPVQPVQHNIIGDLLRKSRKEARRAGRKQKR
                     WEGACKALQELNKYRDLINELSE"
     gene            6946..7128
                     /locus_tag="ACHKFK_004484"
     CDS             6946..7128
                     /locus_tag="ACHKFK_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9054676.1"
                     /translation="MEVNLMTEKEVSDLLQKRRTALYNLRKKHGFPEPVLTHPARYSR
                     QAVEQWLKAGGINRAV"
     gene            complement(7112..7489)
                     /locus_tag="ACHKFK_004485"
                     /pseudo
     CDS             complement(7112..7489)
                     /locus_tag="ACHKFK_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218301.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         2176 a         1708 c         1773 g         1874 t
ORIGIN      
        1 gggaattcac gctgcgatta agcattctga caagcgaaca acctgttctg gtgctacgta
       61 tcgtgcgtgt tgaagctgcg gaagaagaaa ttgccaaaga gttccgtgac ctgctgaaag
      121 agcgtttcga agaagaagat atcttaacct tcatcggcac gttctcagtg tgatgaaatc
      181 tgtgacaggg acgtcacgac atatcgctca tatttcacga gaaaatacac agttcttgga
      241 gggtatacta tgcaatttaa agatttacca gaggatattc agaaaatagc tgcggatacg
      301 cttaaagacc atttatcggt gataaattta acaaaggaac caaaggcgaa tctggaaaat
      361 atatcccgta acatgcgaga tgtttttgtc gggatgtatg cttatgacaa tgaaaagcac
      421 gaggaacata ttcaaaattg ccgccttaat aaaggacaac aaaacatcaa acacaatata
      481 acagaaaacg acaaatcaat taattctgtt gaaagagtga ataattcgca gaaaacagac
      541 ccattggtat tctggaatat cgttcaggct tttgccttga gcaaggtgta tgagtttaac
      601 aacatcaatc attcagggat cgacactcat caatcatcac atcgtgaggg ggtgtccatg
      661 cttctgtctc tgttacgaga gcagggcgaa tgtcctcact cgacattgaa cgacactgag
      721 caatagtcca tccgtatttt ccgccaactt gatatcgtca tcaaacttaa ctttgatcgt
      781 gcgcttccag ttgtgaataa caaccttgct gtgtttagtc attggcggca tcagttgccg
      841 attgctgatg tccgcccttt ttaaagtatt aagggttaac tcatgacggc ttttaccgac
      901 aaagagctaa ttaaagaaat taaagaacgt atcagcagtc tggaggtgcg ggacaatatt
      961 gagcgccgtg tttatgagat tgcgttgact gcattgaccg ctgaaccatt cgccactatc
     1021 gacaccgtgg gaattgagct cgttaaatat ggttgtaaca cgtttatttg tcccgacaat
     1081 tcgatggagc cgggaaatgt accgctatat atcggcctgc cacgaattga gccagcaagc
     1141 cagactgcca agcggtcatt ccaggaatgg ttgtcagaac aaaaagaaaa aatagacgtt
     1201 gattgcggat gtgtaagcat cgaaacgctt acgcactgga tgaaatcagc ttatgaggct
     1261 ggcaactctc cggtaactcc ggatggttgg ataagctgta gtgagcgaat tccggacgat
     1321 aaacagtatg tttggtgttg gggtaagtct tacggctgga ctgagtgcga taccttcgaa
     1381 gggtattacg attggtcgag aaacaaatgg tgggcagtta ctgacgatgg ggaagaaccg
     1441 gcatcgaaag taacccactg gatgccgcta ccagaaccgc cgcaggaggt taattgatgg
     1501 actccttcgc gaaatatacg attattgact ggatagcatt ccttcaggtt ttgctcatct
     1561 ggttttatat ggcttacagg agtggacagt ggattgtcag tgtagcctgt agcaagggat
     1621 ggcgttggtg gaaccgaaag aataaaaaaa gcactggcct tggattcatt ttacgaagca
     1681 ttcaatctta acagccttca gcctggttct gtcgttgtag tcaccactca aagcggcatg
     1741 acgatacaaa ttcacaagcc aaaggaggaa ggtcgtggct aacctgcaac ttgccgtcaa
     1801 aggtgaataa gaatcctcgc actcgcgggg atttctttta tctgaactcg ctacggcgag
     1861 cttgagaggt taaaacgatg aaaaataatg taatgaaaaa tggaactatc gacagccagg
     1921 ctctgttgaa aatggtgaat gaagcgcgga gactgtgcgg tgaaaaagaa gtacgcaaca
     1981 acgacttcat tgcacgcatc aaagatgaac ttgatgggga gggttacgaa attttcgtaa
     2041 cccccatgga taagaaaaaa ggcggtgcgg atcaggttgt tattgtgatg acctataaac
     2101 aagcgcttcg cgtcgccgca cgtgagtcga aagccgtccg ccgttctttt gcgtaacccc
     2161 caacaggaga aatgatatga aatctattat caaacatttt gagtgcgaaa tctcacaggg
     2221 taatatcgtt gggctggatg cagctttcta tcagggtaag cctgtgtttc tggctgctcc
     2281 attcgcaaaa gccctgggat acgccaacac ggcggacgca ttgaaaaagc actgcaaatc
     2341 attgattaaa cttaattatc gcgaatcgcg agaattaggt ttaggtgaca atccgcgagg
     2401 aattcaactt attggccaag gtgacgcttt tcgtctcatc atgcgtagct cgttaccatc
     2461 ggcagaacgg gttcaggatt gggtttgtga agttgtcctc ccagccctga tggagacagg
     2521 aacttacagc cttaaacagg agaaaagtac accatcaaac ggccttcctg aataccgcaa
     2581 agctaaagcg ctgaaaatgg aaatggaggt tatctcaagc gttctggatc gtttaccgca
     2641 cctgggcgac aaggcgaaac aggctgctta cgcctcagtg attaaccgtt ctgctggttt
     2701 taatgttatc ccccttcctg ttcttgatga acatcactac agtgcaacag aggttggtaa
     2761 acagtttggc gtcactgcaa acaagatcgg tcgcattgcc aatacctaca tgctcaaaac
     2821 agaacaatac gggaaatggt tcatcgacaa atccccgtat tcggataagc aggtagaaag
     2881 ctttcgatac aataatcggg gagtacggaa gattgaagaa atcctggagg cggagaacaa
     2941 agcagagttc ggaacctgat atcctccact ctccgacgcc gccagaatga cggcgcggtg
     3001 gttaagcatg aggcgaaatt tctgccgggc ggtattttgt tgagagatga gatacgtcaa
     3061 cttctctttc cgctttttcg aggctccatg ccgtctggag acgaatccat gaagacggag
     3121 ttccacccag gacagcagcg agacgaacag ccatttcagg agatattgtc gcattacctg
     3181 aaaccagtcg ctgaacagtg gaaggcgcga catcaagcgc tcttgccagt tccctgattc
     3241 caatattctg atcttccaga atatcagcaa taatttctcc gggatgaggg gggttaaaca
     3301 tcgccattag tggtaatcct cgtagttaag aatataagca tcgccgttcc gaaactcgaa
     3361 tgtgattctc cagttaccgg agacggtgac agaccatgta tcggatctgc ccccctgcaa
     3421 ctggtgcagc ctgtacccgg caaggttaat atcatcaatg atttcagctt gatctattac
     3481 ctgaaggcgc gttctcaggc gtttaacgtg attgggattt atgcctgatg catttcctgt
     3541 ttcaaagaaa cgttgaagtc ctttgtgttt ccagcttttt atcatgcttg tttcctgttg
     3601 cgttacgcga atcattatag cgactgttgt gtaatgcgca acggttgttt gacaaaaata
     3661 ggtatccggg attatattca ccgcacgcca gcaaaatctg gcgtcgggat taggaacccc
     3721 ggatattcaa ccgcgacaga cacacgccgc gagcgtgttt tttattgtcg tatgcacacg
     3781 cacatctgaa ttatggtggg gcgtatgggg cagccgaaag gctggccggt tggttgatcc
     3841 ggtagttcct aaccctgtac gtctcgccac ccgatgatta ggaacctgac ggtggtgaat
     3901 gaaaaactca tcaaccggag gcgtcactat gacaactcaa gtttctgttg aaacactctc
     3961 cacgattact tacaagcaga tccccgttat cactactgaa cttttggcgc acctttacgg
     4021 cacagaagct atttgtattc gccagaatca ccacgaaaac aaaggtcgtt tcattgagga
     4081 aaaacacttc ttcaaacttg aaggtgaaac tttacgtgag ttcaagcaca gagtagcttt
     4141 taactactct gtgaaaattg cccgtaacgt tcgttccctc atcctctgga ctgaacgcgg
     4201 cgctgcccgt catgcaaaaa tgctcgaaac cgatcgggct tgacgttata ggtcgttact
     4261 tgtagattta tctttgtgag gcgtcagaac ctctctaaag cggacaaaac caaccccgtc
     4321 agtgttggat tttttatgcc tgttattcag tgatagcaca atgtgctgtc acatccccga
     4381 acaatgtcgg gagggcgacg aatacaacac ccgtaagggg aataagtccg cggttcttta
     4441 ggccgttctg aacctcccga caccactatc agaggtggta atttcagaaa aagctaaagg
     4501 aggtcgttat gaccatgcaa ctcgctgtag aatccctcgt cgtcgttact tactctaacg
     4561 tccctgtaat aacaacaggg ttattggcac atctatacaa aactgaagaa aaacatatac
     4621 ggcagaactt caaacgtaat gaatgtcgtt tcattgcagg aaaacacttc tttaaagtaa
     4681 gtggctgtga acttgatagt ttgcgaacct ctcaaagagg tttacaaatt tccccaaaaa
     4741 cccgctccct catcctctgg actgaacgcg gcgctgcccg tcatgcaaaa atgctcgaaa
     4801 ccgatcgggc gtgggaggtg ttcgaaaaac tggaagactg ctatttcagt caaaaaatcc
     4861 cagaacaact tcagcttcca gaatcaacgc tatctatcaa ctaccctcta tcgtggtttt
     4921 cagagcatca cccctactcc atgatgagct atgttgatcg aaaaactctt aacctggacg
     4981 tttccgtgct cttcgatatg ccaagcccaa ctatgcgcat cctcaacgag ctacacagca
     5041 agggctataa cgttgacgcc gctgttgctg aatttaacgc cttcaaacat ctgacggaag
     5101 aaatgcgccg tactctacag gatatttcaa gactgtcaga tcgaaattcc cgaaaaggct
     5161 tctcgttaag cctctgactt ccccaccccg acatcccgtc ggggttttca tatctggaga
     5221 caaaaatttg aaacagatcg ctttctacag gcgttctgga aggcccggtg tcttccgtgg
     5281 gttaaaagaa cgcgtcacct ggatgattca gtctcgtggt cgtcctgtta ctggcagtga
     5341 aatagcagag aaatttggtg tttcactttg cgagttcaac aaggtagctc gcagcctgac
     5401 aaaaggcagt aaggttgtga aaatcaaggc ttcagagtca ttcaccacag acaccggaat
     5461 cgttgatcgc tttttctccc tcgaatcaaa tcctcgtcgt gacacacctc gttcacgcaa
     5521 tgccgttcct ccattcagtc gcagaagccg tgaacacgca gcaaaaaact gccgcgagga
     5581 atacgagcaa aaggccaaac gccgtcgccg actgattaaa gcaggacttt acattgatga
     5641 gtttgaaaag gcgctatgac taacaaatac actctcattt acgcagctcc accttggaca
     5701 ttccgcgaca aagcaaccga tggtcaacgc ggtgcaagtt ttaaatatcc ggtcatgagc
     5761 cttctcgata tctgccgcct cccggtatgg gaactggcag cagataactg cctgttggct
     5821 atgtggtggg tgcctacaca accacttgaa gcattgaagg ttgtagaagc atggggcttt
     5881 cgcctggtga cgatgaaagg attaacctgg aacaaatgcg ggaaaagaca gaccgacaag
     5941 ctggtcatgg gtatgggtag caccactcgc gctaacagcg aagactgcct ttttgcagtg
     6001 aaaggaaatc tgcctgaacg cattaacgcc ggaataattc agtcatttac agcaccacgc
     6061 cttgatcact cccgcaagcc ggatatggcg cgagaaaaac ttgtgcaact tcttggcgat
     6121 gttccccgga tagaactgtt cgcccgccac acctcgcatg gatttgatgt atggggtaac
     6181 caatgcggaa caccatccat tgagatggtt ccgggtattg ttaaatttct ggagaaaacc
     6241 aatgagcgaa aaaacgacgt tgacaaaggc atcaccagtt gaattaaggc agtgtctgga
     6301 aatcgcaaat caacttgcca gaagtggaat acgctttgtt ccaatcccga tcacagcaga
     6361 tgcagaactt catctgtttg gtgaaattct ttcccgaaag ctggatgaac tggaaaagct
     6421 ggtagaagaa gctgacacct caccaatcgc ataacacccc caccgacatt aaaatatcag
     6481 gagaaaaaaa tgaacgccgt actcacagaa ttgaacaaat taggaaaagc aacagccgac
     6541 agcatttcta aaggtctcaa tattgatttg aatgacgtca ttgatacgct gtggaagttg
     6601 aaaaaccagg ggatcgtaaa ggtaaaaaac ggcatctggc aggcagtaga gggagttgga
     6661 agcaaaacag atattgattc agttcaacca gttcagccag ttcagccagt tcagccagtt
     6721 cagcacaaca ttataggtga cctgctacgt aaatcacgga aagaagcgcg ccgcgccggg
     6781 cggaaacaga aacgatggga gggtgcatgt aaggcgttgc aagaactgaa taaataccgt
     6841 gacttgatca acgaattgtc agagtgaacg tagcgggaat ccacgtcctt tagggcgtgg
     6901 atgatatcag gctgttgaaa tacatgtaga aaaggagcgc atactatgga agtcaactta
     6961 atgaccgaaa aagaggtttc tgatttgtta caaaaaaggc gcacagcttt gtataatttg
     7021 cgaaaaaaac acggattccc ggaaccagta cttactcatc cggcgcgata tagtcgtcag
     7081 gccgttgaac aatggcttaa agccggagga attaaccgag ctgtttagcg tgccagaaaa
     7141 ttttatcagc atacaactca tacgcatctt tctgttccga caaccagtcg tgtttattat
     7201 acacggccat aactccccca agttcatgcc ccagcatttt ttcagtgacg tggggcataa
     7261 ttccttcccc agataaattc gttaccagag aacgcctaaa atcatgcgtt ctccattcag
     7321 gaatatcaat tttctccctt aactttttca tgtacaaatt agccgacgaa cgatcaatag
     7381 gtttatctat ttcctgcccg gggaatagca catcaaaacc cgcattaagc aatctctcga
     7441 tataaggttt aacctgagtg aataccggac gccgggtgat gctgccaact tactgattta
     7501 gtgtatgatg gtgtttttga ggtgctccag t
//
LOCUS       DAZGJT010000166         7424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZGJT010000166 DAZGJT010000000
VERSION     DAZGJT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.169"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(111..197)
                     /locus_tag="ACHKFK_004486"
                     /pseudo
     CDS             complement(111..197)
                     /locus_tag="ACHKFK_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450687.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            239..469
                     /locus_tag="ACHKFK_004487"
                     /pseudo
     CDS             239..469
                     /locus_tag="ACHKFK_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            521..1456
                     /gene="ttcA"
                     /locus_tag="ACHKFK_004488"
     CDS             521..1456
                     /gene="ttcA"
                     /locus_tag="ACHKFK_004488"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO9054677.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLETLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1585..2958)
                     /gene="dbpA"
                     /locus_tag="ACHKFK_004489"
     CDS             complement(1585..2958)
                     /gene="dbpA"
                     /locus_tag="ACHKFK_004489"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO9054678.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDVPLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVICFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQSVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLSWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(2988..3161)
                     /gene="ynaL"
                     /locus_tag="ACHKFK_004490"
     CDS             complement(2988..3161)
                     /gene="ynaL"
                     /locus_tag="ACHKFK_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO9054679.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(3436..4419)
                     /gene="zntB"
                     /locus_tag="ACHKFK_004491"
     CDS             complement(3436..4419)
                     /gene="zntB"
                     /locus_tag="ACHKFK_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO9054680.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(4433..4480)
                     /gene="ydaN"
                     /locus_tag="ACHKFK_004492"
     CDS             complement(4433..4480)
                     /gene="ydaN"
                     /locus_tag="ACHKFK_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO9054681.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            4674..5906
                     /gene="dgcM"
                     /locus_tag="ACHKFK_004493"
     CDS             4674..5906
                     /gene="dgcM"
                     /locus_tag="ACHKFK_004493"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO9054682.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSEARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRNEEETALSCRLVLRKLTEAEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSMAKVSISSLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5927..6490)
                     /gene="smrA"
                     /locus_tag="ACHKFK_004494"
     CDS             complement(5927..6490)
                     /gene="smrA"
                     /locus_tag="ACHKFK_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO9054683.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPARNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQALADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6685..>7424)
                     /locus_tag="ACHKFK_004495"
     CDS             complement(6685..>7424)
                     /locus_tag="ACHKFK_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9054684.1"
                     /translation="FKDKNFTLGCFFSFSGGVGIFSTVYLIPVFLGQVRGLNAEEIGF
                     AVCTTGIFQLFSVPFYFWLSKKINLRWLLMAGLGGFVFSMYLFTPITHEWGWQELLFP
                     QAIRGISQQFAMAPIVTLTLGGIPKERLKLASGVFNLTRNLGGAIGIALCGSILNNRT
                     NFHFSRMGEKMVSVPHAVNDFISRSALFFNRSGSDQTSEILASTKLLSQLMLREAQTM
                     AFSDTFLLISGLLFIAFLLVPAMDKSS"
BASE COUNT         2003 a         1893 c         1716 g         1812 t
ORIGIN      
        1 ttcacaaaat atcccctgat agaaaactaa aaaacactgc ataatatcgc tattctggca
       61 cttgcgaaaa attatattct gcacttgttg tagcgttcta tttctccgcc tggcaacgaa
      121 tgcacaatgt ccgccacagt ttctgttcta atttcttcct gtgttaccgc atcctgtacg
      181 ggtagcgcaa caccagcatg ctaagcaagg ctttcccatc agatttcact acgactattt
      241 gggagcgaga tggaatgcag cagtaaaacg tgctggtatt cgccgccgta atccgtacca
      301 tacgcgacat acttttgcct gctggctgtt gacggcagga gcgaacccgg catttatagc
      361 cagccagatg gggcatgaaa ctgcgcagat ggtgtatgaa atttacggta tgtggattga
      421 tgacatgaac gacgaacagg tagccatgtt gaatgcgcgg ttatcgtagt tgcaaagttt
      481 gccctcaata tgccccactt agtaccagag aactgaaata atgcaagaaa atcaacaaat
      541 tacaaagaaa gaacaataca acctgaacaa attacaaaaa cgtctgcgtc gtaacgtggg
      601 cgaagccatt gctgacttca atatgattga agaaggcgat cgcatcatgg tttgcctctc
      661 cgggggtaaa gacagctata ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc
      721 aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag caaccgggct tcccggaaca
      781 cgttctgccc gagtatcttg aaacgctggg cgttgagtac aagattgttg aagagaacac
      841 ttacggtatc gtgaaagaga agattccgga gggcaaaacc acttgctcac tgtgttctcg
      901 tcttcgtcgc ggtatccttt atcgcaccgc aacggaactg ggggcgacga agatcgcgct
      961 gggtcaccat cgtgacgata tcctgcaaac gctgttctta aatatgttct acggtggcaa
     1021 gatgaaaggt atgcctccga aactgatgag cgatgatggc aaacatatcg tgattcgtcc
     1081 gctggcctac tgccgcgaga aagatattca gcgctttgcc gatgcaaaag cgttcccgat
     1141 tattccgtgc aacctgtgcg gttcacagcc taacctgcaa cgtcaggtga ttgctgacat
     1201 gttgcgtgac tgggataaac gttatccggg gcgtatcgag acgatgttca gcgcgatgca
     1261 gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc gatttcaaag gcattaccca
     1321 cggttctgaa gtggttaacg ggggtgatct ggcgtttgat cgcgaagaga tcccactaca
     1381 accagcgggc tggcagcctg aagaagatga aaatcagttg gatgagttac ggctgaatgt
     1441 ggttgaagtg aaataaccag gatagcgccc gatgcgcaag cgtatcgggc tactcttatg
     1501 gaggccggat aagacgcggc cagcgtcaca tccggcaatc ccgaagaaga tgtttactct
     1561 tgcaccctgc aattcaacat ttcattattt taataaccgc acccggcacg tttttccttt
     1621 aatcttcccg ccctgtagct gtttccatgc tttatgagca acagcctgac gcaccgcgac
     1681 ataaacatgc gccggatgca cggcgatttt gccaatatct gcgccatcaa gcccgatgtc
     1741 tcctgtcagc gcacctaata catcacccgg gcgcattttg gcttttttcc cgccatcgat
     1801 acacaacgtt gccatttctg cttccagcgg cacaatggaa ctattcgctg gcggcgtttg
     1861 ccagctaagt tttatctgca acatgtcaga aatgatattg gcccgctgtg cttcttccgg
     1921 ggcacagaaa ctgatcgcca gaccgctatt tcctgcacga gctgtacgac cgatgcgatg
     1981 tacatgaact tcagggtccc acgccagctc aaagttcacc accagctcaa gcgattttat
     2041 atccagacca cgcgcagcaa catcagtcgc gaccagtaca cgggcgctac cgttagcaaa
     2101 acgtaccagg gtctgatcgc gatcgcgttg ctccagatcg ccgtgtaacg acaatgcact
     2161 ttgccctact tcattcagcg cgtcgcagac agactggcaa tcttttttgg tattgcaaaa
     2221 caccacgcaa gaggatggct gatgcaagct taataaccgt tgcaacagag gaattttgcc
     2281 tttgctggat gtctcataaa attgttgttc aataggtggc agagcatctg ttgagtcaat
     2341 ttcaatcgct aaaggatcgc gttgcactct tccgctgatt gcggcaatgg cttccggcca
     2401 ggttgccgaa aacagaagtg tctgccgaga tgctggcgca aaacagatga catcatcaat
     2461 ggcgtcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca gcgtattcaa
     2521 cgcatccagt gatacagtgc ctttttgcag gtgatccagc aaacggcccg gcgttgccac
     2581 gataatatgc ggcgcatgtt gcaacgaatc acgctgcata ccgaacggtt gaccaccgca
     2641 caacgtcaaa attttggtat ttggcagaaa acgcgccagc cgacgcaatt cacctgccac
     2701 ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa atagcggcac
     2761 atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc cggttttcgc
     2821 ctgcacgcga acatcttttc cggcaaggat cgccggaagc gcggcggcct gcaccggcgt
     2881 catggttaaa taacccaact cattaaggtt tgtgagttgg gcgggaggca aaacattcag
     2941 ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga cgcaaagtca gcaggcgcgt
     3001 atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcatc gggtggtggg
     3061 tcaggcatgg gttgcgggcg agggatcgga tcgggtactg gaacaggatc gccaggaatc
     3121 ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc cggtcattgg
     3181 gtggactctt aaagggtaga cgctgataaa taacaggcaa aaaaaagccg actcatcaaa
     3241 gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag taagacaata
     3301 tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagagagata ttgccctgaa
     3361 tgggtagaga gtttattgac ttcgctcaaa ctttacggcg tttttgtata caggcagccg
     3421 gaaaaattgc ttttgttaca accatttact acgatgcaac cataaagcaa caccaccaat
     3481 aagaacaact aacagaatac aaaaaattga aaatccaaat tgccatccgc cgccagggat
     3541 cccaccaagg ttgacgccaa ataaccctgt cagaaacgta ctgggtaaaa agaccattgc
     3601 catcaacgac attgtatagg tacgacgagc taaattttcc tgcatcacct gagcgatttc
     3661 atccgccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaagac cgcgcccaag
     3721 gcgatcggca atatcctgca tccgacggcg ttggtcatcg ctcatccacg gcaaacgttc
     3781 actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca tcacaattaa
     3841 ttgtttgcgc agcagagcca gaaatccacg cggtggaatt tgctgatcga gaagattatc
     3901 ttcgaggtcg ataattttat cgtgcagctg ctcgataaat tcgctggaat gatcggtcaa
     3961 cgcatcgcac acatccacca gccacccccc gcaatcggtc ggaccagtgc cctcttcaag
     4021 atcgctcacc acatcgtcca gcgccagcac tttgcgttgt cgggtcgaaa caattaaccg
     4081 cccgtccata tatacacgca tggcgaccag ctgatcgggg cgttcatcgg tgctgccgtt
     4141 tatacagcgc aatgtaatca gcgtgccttc accgagacgg ctgactcggg gccgtgtgct
     4201 ctcgcccgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg ccagccattg
     4261 ggcgctatca tggtgtacat aattaaggtg gagccagcag ggatgcgctt catcaatcac
     4321 atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg caaatactgc
     4381 atccgggaca ttaacgtccg atcccttaat cgcttccaca gtgcctccat catcaacgca
     4441 ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc cattaccctg
     4501 aaatgattaa taaaattctg tctaaattga atacaaaaag caaaatgctt ttccgtatac
     4561 aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat catatgacgt
     4621 ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taatgggatc tcaatgatta
     4681 cacacaactt caataccctg gacttactca ccagtcctgt ctggatcgtt tcgccctttg
     4741 aggaacagtt aatttatgcc aacagcgagg cgcgactgtt gatgcaagac ctcacgttta
     4801 gtcagctacg aaccggtccc tattccgtct cctcacaaaa agaactgccg aaatacctct
     4861 ccgatctgca aaatcaacac gatattatcg aaatcctcac cgttcagcgc aatgaagagg
     4921 aaacagcatt gagctgtcga cttgttttgc gaaagctgac agaagcagaa ccggtgatta
     4981 ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa agccagtcgc tcggcaaatt
     5041 atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct gcaccaatgt
     5101 tgttgattga cccctcacga gatggccaaa tcgtcgatgc taacctcgcc gcgctcaatt
     5161 tctatggtta taaccatgaa acgatgtgcc agaaacatac ctgggaaatt aatatgctcg
     5221 ggcgtcgcgt catgcctatc atgcatgaaa tctcgcattt acccggtggt cataagcctt
     5281 tgaattttat tcataaactg gcggatggtt cgactcgtca tgtgcagacc tatgccgggc
     5341 cgattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt actgagcaaa
     5401 aacggctgga ggagcagctg gaacatgcag cccaccatga tgcgatgacc ggattactga
     5461 atcggcgaca gttttatcac attaccgagc caggccaaat gcagcacctc gctatcgctc
     5521 aggattacag cttgttgctc atcgacaccg atcgttttaa acacattaac gatctctatg
     5581 ggcattctaa aggtgatgag gtgttatgcg cccttgcccg cactctcgaa agttgcgctc
     5641 gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg ctaccaagaa
     5701 ccccactgga taccgcgctt tcgctggctg aaactatccg cgtaagcatg gcaaaagtga
     5761 gtatttcgag cttaccacgc tttactgtca gcattggtgt ggcgcatcac gaaggaaatg
     5821 aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgagcg aaaaatgatg
     5881 gacgcaaccg cgtgctggcg gcataagcca cggatgcgtc tcgtgatcaa cgactgcgct
     5941 tagcgtggcg ctcccagttt tcttgcttcg cctgcgctgt tttacgtagt gcgacgtaac
     6001 acgccccgct gccgccatga tgcggtagcg cagtgcaata tgcctgaaca tcatcaaatt
     6061 cggtcagcca gcgcgccaca tagctgcgga caatattggc atgcgattta tcatcccgtc
     6121 ctttgccatg aataatcagc acgttacgca aaccatccgc cagggcttgt tgaatgaaac
     6181 tgaacatcat tttgcggcac tcttccaccg gctggcgcaa aagattcagg ctcgcctgtt
     6241 gcggatattt accactgcgc agcttatcca gcaccccatg ttgcaaccct tcccgccgaa
     6301 actccagcgg ctgacttagc gggatgatgt cgagaaatcc ggtggtgagg aaattatcaa
     6361 gttgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgctggatgc cagtggacat
     6421 ctgtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc gatttgtcgt
     6481 caaggttcat gtaacctcct atgcaccttc acacgtcgtg attcagacgg gttaattgtt
     6541 catacttact gcatcgttcc cttcacagat tgaaacaata ctctacttca tacctgtgag
     6601 tatctgcgca ggcggagcat tttatactag cctgttagca gtatcgaaca gtttagcccg
     6661 agtttaattc agcacaggct ccgatcagga tgatttatcc atagcaggaa ccaacaaaaa
     6721 tgcgataaat aacagaccac tgattagcag aaaggtatcc gaaaatgcca tagtttgcgc
     6781 ctccctcagc atcaattgac tcaataattt ggttgaagcc agtatttccg atgtctggtc
     6841 acttccactg cgattaaaaa ataaagcact tctggaaatg aagtcattca ccgcatgggg
     6901 aacagaaacc attttttcac ccattctcga aaaatgaaaa ttagttcgat tattgagaat
     6961 agagccacac agggcaatac caattgcgcc cccaagatta cgtgttaaat taaatacacc
     7021 agaggctaac ttcagccgtt cttttggaat tccccccagc gttaatgtta cgatgggggc
     7081 catcgcaaac tgctgcgaaa tccctctaat tgcctgtgga aacagtaact cctgccatcc
     7141 ccattcgtgg gttatagggg taaaaagata cattgaaaac acaaatccac ccagccccgc
     7201 catcaacagc catcgcagat ttattttttt acttaaccag aagtaaaagg gaacagaaaa
     7261 taactggaaa atacctgttg tgcaaacggc aaaacctatc tcttcggcat ttaatccgcg
     7321 aacctgcccc aaaaacacag gtattaaata tacagtagag aatatcccaa cccctccaga
     7381 aaaagaaaaa aaacatccca gtgtgaaatt tttatcctta aaag
//
LOCUS       DAZGJT010000167         7372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZGJT010000167 DAZGJT010000000
VERSION     DAZGJT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.170"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..607
                     /locus_tag="ACHKFK_004496"
                     /pseudo
     CDS             71..607
                     /locus_tag="ACHKFK_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            565..1242
                     /gene="bglJ"
                     /locus_tag="ACHKFK_004497"
     CDS             565..1242
                     /gene="bglJ"
                     /locus_tag="ACHKFK_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO9054685.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSARREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1280..2068)
                     /gene="fhuF"
                     /locus_tag="ACHKFK_004498"
     CDS             complement(1280..2068)
                     /gene="fhuF"
                     /locus_tag="ACHKFK_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO9054686.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPLMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHVHVEFHETGRAACFWVDVCEDKNATLHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRYALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2209..2445
                     /gene="yjjZ"
                     /locus_tag="ACHKFK_004499"
     CDS             2209..2445
                     /gene="yjjZ"
                     /locus_tag="ACHKFK_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO9054687.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2484..2570)
                     /locus_tag="ACHKFK_004500"
     tRNA            complement(2484..2570)
                     /locus_tag="ACHKFK_004500"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2534..2536),aa:Leu,seq:cag)
     gene            complement(2605..2691)
                     /locus_tag="ACHKFK_004501"
     tRNA            complement(2605..2691)
                     /locus_tag="ACHKFK_004501"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2655..2657),aa:Leu,seq:cag)
     gene            complement(2720..2806)
                     /locus_tag="ACHKFK_004502"
     tRNA            complement(2720..2806)
                     /locus_tag="ACHKFK_004502"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2770..2772),aa:Leu,seq:cag)
     gene            complement(2997..4028)
                     /gene="rsmC"
                     /locus_tag="ACHKFK_004503"
     CDS             complement(2997..4028)
                     /gene="rsmC"
                     /locus_tag="ACHKFK_004503"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO9054688.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDANKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLATNGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            4131..4544
                     /gene="holD"
                     /locus_tag="ACHKFK_004504"
     CDS             4131..4544
                     /gene="holD"
                     /locus_tag="ACHKFK_004504"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO9054689.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPPHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            4513..4959
                     /gene="rimI"
                     /locus_tag="ACHKFK_004505"
     CDS             4513..4959
                     /gene="rimI"
                     /locus_tag="ACHKFK_004505"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO9054690.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            4974..5651
                     /gene="yjjG"
                     /locus_tag="ACHKFK_004506"
     CDS             4974..5651
                     /gene="yjjG"
                     /locus_tag="ACHKFK_004506"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO9054691.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLNVEPGKLNEAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            6037..7254
                     /locus_tag="ACHKFK_004507"
     CDS             6037..7254
                     /locus_tag="ACHKFK_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029397014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9054692.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPATGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGNIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQAWW
                     ERVESIVTCTCSD"
BASE COUNT         1888 a         1753 c         1868 g         1863 t
ORIGIN      
        1 gagctgcatg acaaagtcat cgggcattat ctgaacataa aacactatca ataagttgga
       61 gtcattacct gaagatccac gttccgtttg tgagcattat tactctttaa tctcacaata
      121 tcgggaaatt cactgggttt tcatggtgtc acgctcctgg tattcccagg cagtagaatt
      181 gctcatgtgc cctacggcga cgttattgtc tgatgttgaa cccattgaga atctggtcaa
      241 gaccgtacgt tccggcaata cgcactcaga gcgtatcagc gccatgctga cctctccggc
      301 gatgactgaa actcatgatt ttagctatcg ctccgtcatt ctcactcttt cagagcgcaa
      361 ggtactgcgg ctattaggta aaggatgggg catcaaccag atagcttcat tgcttaagaa
      421 aagtaataaa actattagcg cccaaaaaaa cagtgcgatg cgtcgactgg caattcacag
      481 caacgctgaa atgtatgcat ggataaatag cgcgcagggt gcaagagaac ttaacttgcc
      541 ttctgtttat ggagatgccg cagaatggaa cacagccgaa ttaagaagag aaatgtcgca
      601 ctcatagaaa aatgcgtcat gagtagtatc ggtattgaga gtttattcag aaagtttgca
      661 ggtaaccctt ataagctcca tacctatacc agtcaggagt catttcagga tgccatgtcg
      721 cggatctcgt ttgcagcggt cattttttct ttttctgcca tgagaagtgc gcgcagagag
      781 ggattatcct gcctgactga actggcgatt aagtttccgc gtacccggcg tttagttatt
      841 gcggatgatg atattgaagc tcggctgatt ggttcattgt cgccatcacc gctggacggt
      901 gtattaagta aagcgtcaac gctggagatt tttcatcagg aactcttttt gtcattaaat
      961 ggtgtacgtc aggcgaccga tcgactgaac aatcagtggt acattaacca aagccggacg
     1021 ttaagcccga cggagagaga aatattgcgc tttatgtcgc gtggctactc aatgacacaa
     1081 attgccgagc agcttaaacg caatattaaa acgatccgtg cacataaatt taatgtgatg
     1141 tcgaaactgg gcgtcagttc tgatgcaggg ttgttggagg ccgcagatat tctgttatgt
     1201 atgcggcatt gcgaaacaag taatgtgttg catccttatt aatccgcatg atgccgggtt
     1261 tacttccccg gcagtgcttt catttcagcg tacaatcgcc acattgctgc acatccggta
     1321 agcgataacg ctggcagcaa gtgcggcgca ccagcaggcc gtcgcgtagc accacggtac
     1381 gccagagtgg attatcttca ccggtagtaa gcgttttctc aaaaaagagg gcatagcgca
     1441 gcgattcaac cgtcgcctcg ccgagcagct gtttcatctc agtcagatac cagttgatca
     1501 aataaccggt attgctccag ataagtttgc cgttaatctc tcccgttgct tctagtgcct
     1561 gcacaaccgg aaccagcgcc tggctgatta acgtttccat tcgctgctgc ggcgaatgta
     1621 gtgttgcgtt tttatcttca cacacatcga cccagaaaca ggcggcgcgt ccggtttcgt
     1681 gaaactcaac gtggacatgt tccggcgaca catctaatgc tttttcctga gtcagcagcg
     1741 ccagcattaa tggtggtacc atcaggccga tataccattg cgcccacagt gagatcagcg
     1801 gtttgttttc gcggatcatc aaaggttggt tgcggtagat atgatcggaa tagaccgcga
     1861 gtagagaact tagcgcatta ggtgatgacc attgcgccag cgtcatggcg ttaagtgggg
     1921 cgggttcatc caggcggata aactccagca aatgttcacg atgttcggcg atcgtcgccc
     1981 gcacggcttg tgcaagccca gcatcctgcg gctggagatg cgttcgccag ataacatctt
     2041 catagagcgg tgcggaacga taggccataa tcgggatagt aatctaaatg ataatgattg
     2101 ctaatcctat cgataggttt acccgatagc aagggatttc tctggcttgc aaatgataaa
     2161 aattatcata tgatattggt tatcattatc aatgaaagag atgaaaccat gttgcaacgt
     2221 acgctgggca gtggctgggg agtgttgctg ccgggattgc tgattgcagg gctgatgtat
     2281 gcggatttat cgccggatca atggcggatt gtcattctga tgggattggt attgacgccg
     2341 gtgatgctgt atcacaaaca gttacggcat tacgttttgc taccatcgtg cctggcgctt
     2401 attgctggca tcatgctgat gataatgaat ttgaatcagg ggtgataaaa cagtgaagag
     2461 acaagaaaag aagtaaagat aattggtgcg agggggggga cttgaacccc cacgtccata
     2521 aggacactaa cacctgaagc tagcgcgtct accaattccg ccaccttcgc acagtcatct
     2581 tacttttttt gataccgcct cgtttggtgc gagggggggg acttgaaccc ccacgtccgt
     2641 aagaacacta acacctgaag ctagcgcgtc taccaattcc gccaccttcg cccagtgcga
     2701 gcaatatcaa cgtggttttt ggtgcgaggg gggggacttg aacccccacg tccgtaagga
     2761 cactaacacc tgaagctagc gcgtctacca attccgccac cttcgcatac catcaattct
     2821 taaaaagaat tgctaccacg gaggcgcatt ctagtggttt tcagcttttc gtcaatagtt
     2881 aattatcgac agaggtgtaa ttgctggaaa aatgtccatc agcggattgt ggataattcg
     2941 tttgtcggtc agataaggcg tgtacgccgc atccgacaag gcatgcggcg agatacttaa
     3001 cctttcttcg cctggcgggt cataatggcg cgatacacct tgaagcgccc ggtttgcgca
     3061 atcacttcgt ggaaaccaaa tgtctcatcc agcacgtccg ggtaaggcag gaaggcgttc
     3121 gctacaattc gcaactcgcc gccgctgtta agatgacgca ccgcgccgcg aattagcgtt
     3181 tgcgccgcat ccaggctggt ttgcatccca tcgtggaacg gcgggttgga gattatcata
     3241 tcaaagcgac ctttcacctc ggaaaagacg ttgctggcaa agacttcacc ttcaacaccg
     3301 ttggtcgcaa gtgttgcgcg actggcttct accgctggtg cagaaacatc acacagagtg
     3361 aggcggatct tcggtgaatg gcgcgcaaaa gcaacggaaa gaacgcctgc gccgcagccg
     3421 acatccagca ctttaccttt cgtgtgtgga gttagcgtcg agagcagcaa ctgactgccg
     3481 acatccagac cgtcgcggct aaacacgccc ggcagcgttt tgaccgtcaa gccatcgacg
     3541 ctgtattcgc cccagaattt attggcatca aataccggct gcttttccag acgaccaaaa
     3601 tagaggccgc agcgacgggc gctgtctacc ttattcaacg gtgcataatc ggccagcatc
     3661 tgctcggcgc tgcgtacgcc gctgcggttc tcgccaacga caaaaatatc cgtgcccacc
     3721 ggaagcagag aaagtaaatt catcaactgg aactgggctt ccggtttgtt cttcggccag
     3781 tagtagatca gcgtatcgca atctgcgacg tcatctgccg tggcgaccag gctaaaacgg
     3841 gcgttatccc ccatctggcg gcttaacacc tgccagtggt ggaattgctg ggtatgagca
     3901 cggctggccg cggtatctaa acgcgcgggc aggtcatcct gtaagtctcc ggcaaacaga
     3961 atacggcttt gttcgaaatc atcactgtga cgcagcaaga cttcacttgc cggggtaaat
     4021 gcagacatgg aatgctcctc aattgatact ggcggcgatt atagccatat gttggcgcgg
     4081 tatcgacgaa tttgctatat ttgcgcccct gacaacagga gcgattcgct atgacatccc
     4141 gacgagactg gcagttacag caactgggca ttacccagtg gtcgctgcgt cgccctggcg
     4201 cgttgcaggg ggagattgcc attgcgatcc cgccacacgt ccgtctggtg atggtggcaa
     4261 acgatcttcc cgccctgacc gatcctctgg tgagcgatgt tctgcgtgcg ttaactgtca
     4321 gccccgatca agtgctgcaa ctgacgccag aaaaaatcgc gatgctgccg caaggcagtc
     4381 gctgcaacag ctggcggttg ggtactgacg aaccgctatc actggaaggc gctcaggtgg
     4441 catcaccggc gctcaccgaa ttacgggcaa acccaacggc acgcgccgcg ttatggcaac
     4501 aaatttgcac atatgaacac gatttcttcc ctcgaaacga ctgatttacc agcggcttac
     4561 cacattgaac aacgcgccca cgcctttccg tggagtgaaa aaacctttgc cagcaaccag
     4621 ggcgagcgtt atctcaactt tcagttaacg caaaacggca aaatggcggc gtttgcgatt
     4681 acgcaagtgg tgctggatga agctacattg ttcaatattg cggtcgatcc tgactatcag
     4741 cgtcagggat tgggaagggc gctgctggaa catctgatcg acgaactgga aaaacgcggc
     4801 gtggcgacgc tatggctgga agtccgtgct tcaaacgctg ccgccattgc cctgtacgaa
     4861 agtttaggct ttaacgaggc gacaattcgc cgcaattact accccaccac ggacggtcgc
     4921 gaagacgcca tcatcatggc gttgccaatc agtatgtaag acaaggtgga ataatgaagt
     4981 gggactggat tttctttgat gccgatgaaa cgctgtttac ctttgactca ttcaccggcc
     5041 tgcagcggat gtttcttgat tacagcgtca cctttaccgc tgaagatttt caggactatc
     5101 aggccgttaa caagccactg tgggtggatt atcaaaacgg cgcgatcact tcattacagc
     5161 ttcagcacgg gcggtttgag agctgggccg aacggctgaa cgtcgaaccg ggtaaactca
     5221 acgaggcctt tattaatgcg atggcggaaa tctgtacgcc gttgccgggg gcggtttccc
     5281 tgcttaacgc cattcgtggc aacgccaaaa tcggcatcat caccaacggc tttagcgcct
     5341 tgcaacaggt acgtctggaa cgcacgggcc tgcgtgatta cttcgatttg ctggtgattt
     5401 ccgaagaagt tggcgttgcc aaaccgaata agaaaatttt cgattatgcg ctggaacaag
     5461 cgggcaatcc tgaccgttca cgcgtgctga tggttggtga cactgccgag tccgatattc
     5521 tcggtggcat caacgccggg cttgcgacct gctggctgaa tgcgcacaat cgcgagcaac
     5581 cagaaggcat cgcgcccacc tggaccgttt cttcgttgca cgaactggag cagctcctgt
     5641 gtaaacactg attgcctccc ccccgttgat gggtaaaata gccgcaattt ttcgttttca
     5701 acaagcgcgg cgcgatgccg cttactcaag aagaaagaat tatgacgttg tctccttatt
     5761 tgcaagaggt ggcgaagcgc cgcacttttg ccattatttc tcaccccaat aattaagccc
     5821 aaattaaagc tcttttactc tttcaaaatc ctttcagtta attgagtaat aattacacaa
     5881 ctcatttaat aacatcatgt tatgtggttt ctttaattga aagttctttc atgtaacttt
     5941 aaatcgatca ggttactttc atcaaaaatc tgtacatgtg cttgtacata atgtacaaaa
     6001 cagcagaggt gttttgttat ttgtacaagg tgagtaatgg cgctgtctga tgcgtggttg
     6061 cgttcagtcg ttggaaagga acgtgataag gttttggtta aatccgatcg tgatggtctg
     6121 tctgtcagag tatcaccgaa aggtcgcgta gtgttccaat atcgttatca atgggcaggg
     6181 aaaggtgagc gtcttgatat cggaacttac ccggcaactg gattaaaaga ggccagagaa
     6241 gaagttatcc gtctccgtgg tgaactcgag tcaaaccgta atccacgatt ggtcaagcag
     6301 gctgaaaaac gaaaagctac tgaagccatg acggtagagt ctgtgatccg tgcctggtat
     6361 gaagcatatt gtgtaaaaaa taaaaagggt tctgaacaga tactccgctc gtttgagctg
     6421 cacctgttct ctaaaatcgg gaatatccct cacgatgcag ctacattgca tgattggtta
     6481 gaagtcttgg agcctcttag cactaagact ccagcaatag cagaccgatt gctaattaac
     6541 gcaaagcagg cccatgtctg ggcgtataag agaaagctta ttgaaactcg cccgctgtcg
     6601 gatatcacgg gtaaagatat ggatatccgt aaaggtcaga agaaacggtt tctgacacac
     6661 gatgaaatta aaattcttta tgctgcgatc gatggctcta gaatggttcc caaataccgg
     6721 gccttcatta aactattact gcattttggt tgccgtagtt cggagctaat tactgctagg
     6781 gtggatgatt ttgatttcat aaataaggta tggactgtac cgccagaacg acataagaca
     6841 ggggatataa caggcgaacc gctaaagcgc cccattattg aaccggttga agagcttata
     6901 aagtacgtta tctctatgaa caatggttcc gatatgcttt ttactaagga aggaagcagg
     6961 gagcccgttg gacggacatc attgcagtcg ctgccttaca atttaatgca gtacgcatgg
     7021 cggcgtttgg ggtatcaatt ccctcattgg tctcttcatg atttgagacg aacagcacga
     7081 acaaactttt ctgatcttac tgcgcctcat attgcagaaa taatgctcgg tcataaactg
     7141 ccaggggtat ggcaggttta tgacaagagc gattatttag aagaacagcg taaagcttac
     7201 caggcgtggt gggagagggt tgaatcgata gttacttgta cttgttcaga ctagaactga
     7261 cattttgcat aggcagaacg caattatgta tattgcggta taatcccctt cttaaaactg
     7321 taagaattgc ttgctaatcc ttgcagtttt tgattataac atttttattt gt
//
LOCUS       DAZGJT010000168         7082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZGJT010000168 DAZGJT010000000
VERSION     DAZGJT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.171"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..301)
                     /locus_tag="ACHKFK_004508"
     CDS             complement(<1..301)
                     /locus_tag="ACHKFK_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054693.1"
                     /translation="MVDIVEADKTDIYFIQESVYGKIGLPAFGNTIGPSAQQVVKKVF
                     AVVKERDKTHAKQRLLLEYNGNKLWMNAIDGSEAILPTEFSKRYELSLFNTTNFGE"
     gene            complement(530..1294)
                     /locus_tag="ACHKFK_004509"
                     /pseudo
     CDS             complement(530..1294)
                     /locus_tag="ACHKFK_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1570..3663)
                     /locus_tag="ACHKFK_004510"
     CDS             complement(1570..3663)
                     /locus_tag="ACHKFK_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2235 domain-containing protein"
                     /protein_id="HFO9054694.1"
                     /translation="MSSKISGLAFPCYAPPVFPEDGRLILSEAQVNANYLKQINKTEE
                     HKTNCSKQAGFRIGFTCNQSLHISLFFDGTNNNEYNDTPGHPTNIAKLFHTTYQNAEE
                     QGYFNYYIPGVGTPFPKIGEMDYSNSGLEFATGGEDRINWALLRLVDALSYSIDPSHK
                     RLDDNVAKEHIPLMRAPWPMTGEVNRRNVINPYLEKLQGLLEQASPRLLNVKLFIYGF
                     SRGAAEARTFVNWLTPLACPQEQQVMLAGLAVSIEFLGLLDTVASVGAAHVLPGAAGH
                     MGWADSTQQLPDERQFPGLIKCCRHFVAAHEQRLCFPLDSIRRPDGNYPLNAEEIIYP
                     GMHSDVGGGYPVRDQGKSCGDSGDILSQIALHDMYLAAFDSGAPLAVYSKFVTPLIKG
                     VSPLRIMSPSSVKEFTIANSLTKRFNIWRQTLLNTTLQGTEEMIDTREGYHPYQLAQV
                     LESAISDQIGWITAWRIGRYANNSLLVQPFYIKAPQLDKDGLDKEAQAREEQMENIEK
                     ERVNKQKGNGTLDESNQGIPILDPTNAQYQLREAAREFQSDYKGWTRDINGSVTERAI
                     QILVDVIPKYPVYLINGDDEAKEYQQIKAMGDGLYPQFFIDSLGTGTRTQPCAELLAL
                     YDDQVHDSRAWFMQSSLGGREPWGGYFRYRMIYFGDEANKELKLISADGEVVGDQPTS
                     NRVIYWMESKTVSRG"
     gene            complement(3648..4190)
                     /locus_tag="ACHKFK_004511"
     CDS             complement(3648..4190)
                     /locus_tag="ACHKFK_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001518352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9054695.1"
                     /translation="MSSKIRNGMNITKVVKGCLVLILMVISGCSAANYDDYTAGDLRA
                     INHIKGISINWFTVNGYRVHGLGGGYCCIMLPEKWHSGLKARIEWEVDPTPLEPIAMK
                     KEGFGYEEDAYAKHAANYQKYKVIVDIPEYGAERCGLTVHFLPCNQIKVTTVCEGYGT
                     PNYPIKEPREMKEPATCPAK"
     gene            complement(4175..4729)
                     /locus_tag="ACHKFK_004512"
     CDS             complement(4175..4729)
                     /locus_tag="ACHKFK_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO9054696.1"
                     /translation="MSIKIRKSISMRAIFKCYSLMILFAVSGCSMARNSGDYTAVDIN
                     GINHTDKAINYIHINGYGGPNIGPFGDGGGYCCVMLPDKWHSGLKARVEWEVDPNTAP
                     LPPGYKDREKFEAWKKVALNSFRKYSTIVDIPEYDAELCGLTVHFLSCNQIKVTTVCQ
                     KYGTPNYPIKEPREMKEPATCPAK"
     gene            complement(4714..5267)
                     /locus_tag="ACHKFK_004513"
                     /pseudo
     CDS             complement(4714..5267)
                     /locus_tag="ACHKFK_004513"
                     /inference="COORDINATES: protein motif:HMM:NF023173.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
     gene            complement(5695..6471)
                     /locus_tag="ACHKFK_004514"
     CDS             complement(5695..6471)
                     /locus_tag="ACHKFK_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4123 domain-containing protein"
                     /protein_id="HFO9054697.1"
                     /translation="MITVSPHATDQDKYIIWLKQAESFCSAAGLDYIDVLVDQAGTDQ
                     PLQNEMLHLWPAIHWYALFEGTPEAETMEYSPVVLRLYFALSSHRKFLAQLMASFADT
                     PRLTLLISPLTFDLLGRHLQVLSQVQWEEQTGLLRYYDNRVFPLLFTHVLTNEQQATF
                     TDIALFWSWLDRDGEINWKSGSFSPERRLTDNPEMNRVDDVQVGLMGCICDAEALMKE
                     KAVSELSQESYFSHCLDIAIRANDAGIFRGFAGLRGIILW"
     gene            complement(6484..>7082)
                     /locus_tag="ACHKFK_004515"
     CDS             complement(6484..>7082)
                     /locus_tag="ACHKFK_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HFO9054698.1"
                     /translation="HDQSITVANDRCITVRNDQTLQVTNDRTVSVSNDDGLYVRNDRK
                     VTVEGKQEHKTTGNHVSLVEGKHSLVVKGDLARKVSGALGIKVDGDIVLESSSRISLK
                     VGGSFVVIHSGGVDIVGPKINLNSGGSPGAPVPTLQPAVLKALADDKSLAAEQNDSNA
                     SSDQNDNNDEGEEAPEKSVCKECLKRAQEAAAAFAPRG"
BASE COUNT         1849 a         1786 c         1394 g         2053 t
ORIGIN      
        1 cttctccaaa attagtagta ttgaataatg aaagttcgta tcgcttagaa aattctgttg
       61 gtaggatagc ttcactgcca tcaatagcat tcatccagag tttgttacca ttatattcca
      121 acaataatct ctgttttgca tgtgtcttat ctcgttcttt aacgacagcg aatacctttt
      181 taacaacttg ctgtgcggat ggtcctattg tattaccaaa agctggcaac ccgattttgc
      241 cataaacact ctcctgaatg aaataaatat ccgttttatc ggcctcgaca atatctacca
      301 tttatccctc caaacaagac ctgactccag gacaagagca tagtgtgtag taaacacttc
      361 atgctcttgc cctgaccatt gaaataacaa ctttcgtttg tttactgtac gctgtctaaa
      421 tagctgcgtg gaataaagtt gcacttttta tcatcccttg cagagcaaga atacgaaagc
      481 ccgcccccgc aaggactgac gcgaggtagt ctctgtccgc agctgctttt cacatcttac
      541 atcttgcccc tgccttgagt atcttatctt tcgttaaaat attaatagcg atcttccgta
      601 tccctgaaaa taattctgct gcatttcctc ttcttatttt gaagtcgtct ttattcatta
      661 ccacgtccag atgccagtgc agcttattct ccacgtacca gtgatttcgg ttcgctgtgg
      721 cgaacttctc tgcggttaaa tcagcagaaa tgctataata tctgtccgtt atttctggct
      781 ctttcttttg ttctgttatt attgaacgaa aggagactgc catgcataat ttcttcagct
      841 ctttccattc aaacgtgaaa tcaataagtt catcagggac atcgcaaata atatgaagac
      901 ggattttaat gacataaatc gctcccttgc ggcgactctt gtcataagag tgccgaagcg
      961 tttttccatc aattgcaatg acatctttat catctgagga atggcagtca cgcatccagt
     1021 taataaagca ctcatgaaat tttgcaggac tgatacagga tacaactctg gcaatgatat
     1081 cgtgaacagg aataccattt tcaaaatcac catatcactt caaaaaatag ggatgtgttt
     1141 ccccaaatcc tctatatctt cccagcctta tgcaccaaaa ataacggcgc aaatagttaa
     1201 cagatagaat atctggcaat ttatgttcca ctttccaggc ttgcctggaa tcggggataa
     1261 tagaaatatg ttccatcaat tttttaagat ccattttgcc ccccttaatt atgcaatgag
     1321 tatttgatca tgcataagca atgaaaaaca gctttaggta ataaggaaca cctcaattat
     1381 tgaacataaa atgcgaatta tttagtacaa aaagtgtact cctgatgatc tttccctgat
     1441 tacttgacct ttccctggga gtacagagtg aagtttccgg catggcggga tgcggatggg
     1501 tttgaactgg ggtgattagg cactacatgt ctacattttc ccggtctcca acccctcaat
     1561 cttatgaact taacctcggc tcaccgtttt cgattccatc caataaatga cccggttgga
     1621 tgtgggctga tcgccaacaa cttcgccatc tgcagagatt agcttcagct ctttattagc
     1681 ctcatcaccg aagtaaatca tgcggtagcg aaaataaccg ccccagggtt cgcgtccacc
     1741 cagcgaactt tgcataaacc aggcgcggga gtcgtgaacc tggtcatcat agagagccag
     1801 taattcagcg cagggctgag ttcgcgttcc cgttcccagt gaatcaataa aaaattgtgg
     1861 atagagtccg tctcccatag ccttaatttg ctgatattct tttgcttcat catcgccatt
     1921 aatcagatac accggatatt taggaatgac atccacaaga atctgaatgg ctctctccgt
     1981 gacgctgccg ttaatatcgc gcgtccatcc tttataatcg ctctgaaatt ctcgggctgc
     2041 ctctcgcaac tggtactgag cgttcgtcgg gtcaaggata ggtatgccct gattactttc
     2101 atcaagtgtg ccattgcctt tctgcttgtt cacccgctct ttttcgatat tttccatttg
     2161 ttcttcgcgc gcttgggcct ctttatcaag tccatcttta tccagttgtg gcgccttaat
     2221 ataaaaaggt tgtacaagaa gactgttatt cgcatagcga ccgatacgcc aggcggtgat
     2281 ccagccgatc tggtcactaa ttgcgctttc aagaacctgg gcaagttgat aggggtgata
     2341 accttccctc gtatcaatca tttcttctgt gccctgcaaa gtggtgttca gcagcgtttg
     2401 tcgccagatg ttgaagcgtt ttgttaatga attagcaatc gtaaattcct ttacgctaga
     2461 aggactcata attcgcagag gcgacacacc ttttattaat ggagtaacaa atttagaata
     2521 aacagcaagt ggtgctcctg aatcaaatgc agcaagatac atatcatgca gggctatctg
     2581 agaaagaatg tcgcctgagt caccacagga cttcccctga tcgcgaacgg gatagccacc
     2641 accgacatca gagtgcatcc ccggatagat aatttcctct gcatttaaag gataatttcc
     2701 gtctggacga cggattgaat ccagcggaaa gcacagccgc tgctcatgcg cggcaacgaa
     2761 gtggcggcag catttaatca ggccgggaaa ctgcctctca tcagggagtt gctgagtact
     2821 atcggcccat cccatatgac cggcagcgcc cggtaaaaca tgcgccgccc ccacagaagc
     2881 tacggtatcc agcagcccca aaaactcaat actgacagct aacccggcca gcatcacctg
     2941 ttgctcctgc gggcaagcta acggagtcaa ccagttaaca aaagttcgtg cttccgctgc
     3001 gccacgggaa aatccgtaga taaacagctt tacgtttagc aggcgcggac tggcttgctc
     3061 taacagtccc tgtaattttt ccaggtaggg gttaatcaca ttgcgacgat tcacttcacc
     3121 agtcataggc cagggagcgc gcataagagg aatgtgctct tttgccacat tatcatccag
     3181 tcgcttatgg gaagggtcaa tggaatagct cagggcatcc acgaggcgta ataatgccca
     3241 attaattcga tcttcaccac cagtagcgaa ctccagcccg ctgttgctgt aatccatttc
     3301 acctatcttg ggaaatggtg tgcctacacc cggtatatag tagttaaaat atccctgttc
     3361 ttctgcgttc tgataggtcg tatgaaacag tttggcaata ttggttgggt gtccaggagt
     3421 gtcattatat tcattattat ttgtaccatc aaaaaacaga ctaatatgca gactttgatt
     3481 acacgtaaag ccaattctaa atccagcctg tttactacag ttggttttgt gctcttctgt
     3541 tttattgatt tgttttaggt agttcgcgtt tacctgcgct tcagataata ttaatcgccc
     3601 atcttcaggg aacaccgggg gggcataaca cggaaaagcc agaccactta ttttgctgga
     3661 catgttgcag gttccttcat ctcgcggggt tctttgatag gataattcgg tgtaccatag
     3721 ccctcgcaga cggttgtcac tttaatctga ttacacggca gaaaatgaac ggtcaggcca
     3781 caacgttcag cgccgtattc cgggatatca acaattactt tatatttttg ataatttgca
     3841 gcatgtttag cataagcatc ttcttcatag ccaaaacctt cttttttcat tgcaataggt
     3901 tcaagggggg tcgggtctac ttcccactcg atccgggctt ttaatccaga atgccacttc
     3961 tctggtaaca ttatgcagca atatccaccg cccaaaccat ggacccgata accatttacg
     4021 gtgaaccaat taatactgat acctttaata tgatttattg cccggagatc acctgcagta
     4081 taatcgtcgt aattagctgc tgagcaaccc gaaatcacca tcaaaattaa taccagacag
     4141 cctttaacta cttttgttat attcattcca tttcttattt tgctggacat gttgctggtt
     4201 ccttcatctc gcggggttct ttgataggat aatttggtgt gccatatttc tggcagacgg
     4261 ttgtcacttt tatctgattg cacgatagaa aatgaacggt caggccacaa agctcagcat
     4321 catattccgg gatatcaaca attgttgaat attttctaaa actgtttagc gcaactttct
     4381 tccatgcttc aaatttctcc ctgtccttat agcctggagg cagaggagct gtatttggat
     4441 caacctccca ctcaactcgg gcttttaatc cagaatgcca cttatctggc agcataacgc
     4501 aacaatatcc tccaccgtcc ccgaatggac caatatttgg tccgccgtaa ccattgatgt
     4561 gtatataatt tatagcctta tccgtatgat ttatcccgtt aatatccaca gcagtgtaat
     4621 caccgctatt tctcgccatt gaacagcctg atactgcaaa taaaatcatt agagaataac
     4681 atttgaaaat cgctctcata cttatacttt tccttatttt gatggacatg ttgctggttc
     4741 cttcatctcg cggggttctt tgatgggata atttggtgta ccataccctt ggcagactgt
     4801 tgtcactttt atctgattgc atggcagaaa atgaacggtc atgccacaac gctcagcacc
     4861 gtattccggg atatcaacaa ttgcggtatg ttgaatgtaa ctttctttga gttttttctc
     4921 ccaggcctga tatttatccc aatccttata gagtggtggc agagggactg tatttggtgt
     4981 aattccccac tcaatccggg cttttaatcc agaatgccac ttatctggca acataacgca
     5041 acaatacccc cccgctcccc gaacggaact atattttgcc cgccatagcc attgatactg
     5101 aaataattaa tagcaacatc ggtatggttc atacctttaa tatctccagc agtgtaatca
     5161 ccgctatttc tcgtcattga acagcctggc actgcaaata aaactattag cgaaaaatat
     5221 ttaaaaatcg ctctcacacc aataccattc tttatttcga cgggcatatt gctggttcct
     5281 ttactttgcg agtttttgat taattggtat tatttctacg acaacaaagc tttgacaaag
     5341 aagcaaataa aacacatatc tttcgattgt cacacattgg gatatcgaca gagcattgat
     5401 gatattacgc aaaatatatt gcaaccacgt gaactgacat tacgatcgaa cacaaatgca
     5461 acatgatgga tactaccatt acatgtcgag cttgattttc cgtttatttc acgattatct
     5521 cttgatatta acagctacgg aattttaaaa agataaatat acacatcgtc cttcaagctg
     5581 actctgcgtt ggctgcgttc actcacccca gtcacgtact tatgcacgct cctggggatt
     5641 cgcccgcttg ccgccttgat gcaacttgaa tgattttgtg tatacccctg tacatcacca
     5701 caatattatc cctcgaagtc ccgcaaagcc ccgaaatatc cccgcgtcat tggccctgat
     5761 agcaatatca agacagtgcg aaaaatagct ttcctgactg agttcactga ctgctttctc
     5821 cttcatcagg gcttcagcgt cgcagataca gcccatcaat cccacctgta catcatccac
     5881 ccgattcatc tccgggttgt cagtaagcct gcgttctggt gaaaaacttc ctgatttcca
     5941 gttaatctct ccatcacggt ctaaccagct ccagaacaac gcaatatcag taaatgtcgc
     6001 ctgctgctcg ttggtcaaaa catgtgtgaa cagtaaaggg aacacccgat tatcatagta
     6061 acgcagcaat ccggtctgtt cttcccactg aacctgtgac aatacctgaa gatgccgccc
     6121 cagcaggtcg aatgtaagag gggatatcaa caaagtaaga cgcggcgtat cagcaaatga
     6181 cgccatcagt tgtgccagaa atttgcgatg actactgagc gcaaagtaca ggcgtaacac
     6241 tacaggggaa tactccatcg tctcagcttc aggcgttcct tcaaacaggg cataccagtg
     6301 aatggctggc cacagatgaa gcatctcatt ttgcaaaggt tggtctgttc cagcctggtc
     6361 aacaagcaca tctatgtaat ccaaaccagc ggcagaacag aatgattcag cctgcttcag
     6421 ccagataata tatttgtctt gatcggtagc gtgtggcgat actgttatca ttgtctttct
     6481 cccttatcca cgaggcgcaa aagcggcggc tgcttcctgc gcccttttca ggcactcctt
     6541 acacaccgat ttttctggcg cttcctcacc ctcatcgttg ttatcattct gatcggacga
     6601 tgcattacta tcattttgct cagccgctaa acttttatca tctgccagcg ctttcagaac
     6661 cgccggttgc agcgtcggca ccggcgctcc cggactaccg ccgctattga gattgatttt
     6721 cggcccgacg atgtccacgc cccctgaatg gatgaccaca aacgagccac ccaccttcag
     6781 gcttatccgg ctgctgctct ccagcacgat atccccgtcg acctttatcc ccagcgcgcc
     6841 actcaccttc ctcgccaggt cccccttcac caccaggctg tgctttccct ccaccaggct
     6901 gacatggttc cccgtcgtct tatgctcctg cttcccttcc accgtcactt tacggtcatt
     6961 tctgacgtac aggccgtcat cattactcac gctcaccgtc cggtcattcg tcacctgcag
     7021 cgtctggtca ttacgtaccg tgatgcaccg gtcgttcgcc accgtgatac tctggtcgtg
     7081 gc
//
LOCUS       DAZGJT010000169         7071 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZGJT010000169 DAZGJT010000000
VERSION     DAZGJT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.172"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1058
                     /gene="hyxB"
                     /locus_tag="ACHKFK_004516"
     CDS             <1..1058
                     /gene="hyxB"
                     /locus_tag="ACHKFK_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="outer membrane autotransporter HyxB"
                     /protein_id="HFO9054699.1"
                     /translation="DLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFV
                     KNTHKASNASSVIQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQK
                     HTTAGNASYDLDVNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSF
                     HAYLSRQYNNGIFIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAIVKGGYTWK
                     DGNGLFIQPYAKLSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNATVK
                     PYLNLAALNEFSDGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKG
                     DDIENPLQGVVGINVTW"
     gene            1242..1910
                     /gene="ykgH"
                     /locus_tag="ACHKFK_004517"
     CDS             1242..1910
                     /gene="ykgH"
                     /locus_tag="ACHKFK_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFO9054700.1"
                     /translation="MNEQIKQDIDLIEILFYLKKKIRVILFIIAICMAMVLLFLYINK
                     DNIKVTYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYTSEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLTLGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(2153..2848)
                     /locus_tag="ACHKFK_004518"
     CDS             complement(2153..2848)
                     /locus_tag="ACHKFK_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO9054701.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLTQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGIENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(2841..4268)
                     /locus_tag="ACHKFK_004519"
     CDS             complement(2841..4268)
                     /locus_tag="ACHKFK_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO9054702.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(4279..4998)
                     /locus_tag="ACHKFK_004520"
     CDS             complement(4279..4998)
                     /locus_tag="ACHKFK_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO9054703.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNTLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(5526..6380)
                     /gene="rclR"
                     /locus_tag="ACHKFK_004521"
                     /pseudo
     CDS             complement(5526..6380)
                     /gene="rclR"
                     /locus_tag="ACHKFK_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
     gene            6607..>7071
                     /locus_tag="ACHKFK_004522"
     CDS             6607..>7071
                     /locus_tag="ACHKFK_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO9054704.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPGGNLEIHGEKIFINTGAQAVVPPIPGITTTPGVYDSTGLLNLK"
BASE COUNT         1925 a         1741 c         1542 g         1863 t
ORIGIN      
        1 tggatcttat caatgaacaa acggcattta tttctaccgg caaagacagc actctaaaag
       61 ccacaggcac aacggaaggt ggtctgtatc aatatgacct gacacaggga gctgatggta
      121 acttttattt cgtaaaaaac acgcataaag catccaacgc cagctccgtg attcaggcaa
      181 tggcagctgc tccggctaac gtcgctaatc tgcaggctga cacgctctcc gcccgtcagg
      241 atgctgtccg tctgagcgaa aatgacaagg gtggcgtatg gattcagtac tttggcggta
      301 aacagaaaca taccaccgcg ggaaatgcat cctatgacct ggatgtaaat ggtgtaatgc
      361 tgggtggtga tacccgcttc atgactgaag atggtagctg gctggccggt gtggcgatgt
      421 cttctgcgaa aggtgacatg actaccatgc agagcaaagg tgacactgaa ggttacagct
      481 tccacgctta cctgagccgc cagtataaca acggtatctt cattgatact gctgcacagt
      541 ttggtcacta cagcaacacg gcagatgttc gcctgatgaa tggtggcggt accatcaaag
      601 ctgactttaa caccaatggt tttggtgcga tagttaaagg cggttacaca tggaaagacg
      661 gtaatggcct gtttattcag ccatatgcca aactgtctgc tctgactctg gaaggtgtgg
      721 attatcaact caacggcgtg gacgttcatt ctgacagcta taactctgtg ctgggtgagg
      781 ccggtacgcg cgtgggttat gacttcgctg tgggcaacgc gaccgttaaa ccttatctga
      841 atctggccgc actgaacgaa ttctctgatg gcaacaaagt ccgtctgggt gatgagtctg
      901 tcaatgccag cattgacggt gcagcattcc gcgtgggtgc aggtgtacag gctgatatca
      961 ccaaaaacat gggagcatat gcaagccttg actacaccaa aggtgacgac attgagaacc
     1021 cgctacaggg tgtagttggt atcaatgtga cctggtaaga ataaagcagt cggttcccct
     1081 gtcgactgtt ataccccctg caggcagcag tatgctgcct gcaggctata aatatccagt
     1141 attcatgatg ctggctttgt gctgatattt accctgaaca ttcctgatac agactattaa
     1201 tacaatctta tctgcgacag atttcatttt ctggatatat tatgaatgaa caaatcaaac
     1261 aggatatcga tctgattgag attttatttt atctgaagaa aaagattcgt gttatccttt
     1321 ttattatagc tatctgtatg gcgatggtgc tgttgtttct gtatatcaat aaagataata
     1381 taaaagtgac ttacagtcta aaaataaacc agacaacacc aggcatactt gttagctgtg
     1441 atagcaataa taactttgcc tgtcagacca caatgaccga agatgttatt cagcgaatta
     1501 ctactttttt tcagaccagc ccagatgtca agaacagaga aataaagctg gaatggtcag
     1561 gagataagag agatttacca actgctgaag cggaaatatc tcgcgtgcag gcgtctatca
     1621 ttaaatggta tacgtcagaa taccataatg gcaggcaagt tctcgatgag atacaaacgc
     1681 cttcagcaat taacagtgag ctttatacaa aaatgatata cctgaccagg aactggtcac
     1741 tgtatccgaa cggtgatggc tgtgtcacta taagctcacc agaaataaaa aataaatacc
     1801 ctgctgccat ttgcctgact ctgggatttt ttctaagtat tgtgatttct gtaatgtttt
     1861 gccttgtcaa aaaaatggta gatgaatacc aacaaaactc tgggcagtaa taaacagcat
     1921 tctgacagag agtgcctgta taaaactata gtgacagtga ccgggaaggg agcccgtctt
     1981 caggaatgga ttcctgtctt tttaactgta aatatcctgg cattattaat gtcaggatat
     2041 ttacagaaga gcagcataac catgacggaa cggggttaag tcaggttgtc cttaacgtta
     2101 ttcacaacct cgtataccgc cttcaggttt atgtccagac tacatatctc tctcagcaat
     2161 cctcaataat cagatacacc gctttcaccg gaccatgaac cccgacgact ttgataagct
     2221 caatgtccgc cgttgaactg ggaccactga tgatgttaat acatgacggc attcgttcac
     2281 cggcctgcgc tttctgatgc aatttttctg cgagttgcgc tacacgcggc aggattgtgc
     2341 ttttacgcag gataaaaaga gaagattccg ggagcaggct caatgaacgc ccgcgcccgg
     2401 cggcggaaaa aagaaccacg cctcccgatt cggttaaacc atattcagca tatacgacgc
     2461 ccactttagc ctgctctgct tgtgatatgt tctcaatacc tttcgccgga tcccaaacaa
     2521 cggcattgca ttcctgctgc aaacgttcgc taatccccaa ttcctccagc ctcgtgtcac
     2581 cactaatcac gaccgactga tctcccagct ctttacacag acgtattgca gcttctgccg
     2641 ccttcgcctc gctggtcagt tcacagcgcg tcaacataac atcgctggca aactggataa
     2701 acgcgtcaca gcgctgttgt tgggtaagtt gggtaagccg ctcgttagca tagttgttaa
     2761 gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt
     2821 tattcaaaaa ttcgctccga ttatccattc tttttctcct gcgcctgatg tttcttaaac
     2881 caactacgga aactctctcc gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta
     2941 atcgcgccaa atttgagtgg tgttttgccg ccattgataa accagcttgc cgcatgagca
     3001 ccagccatca tcccaacttt ccacaatcct ggatgactat tggcataagc gaacattttt
     3061 atcgcccgtt gctctgcttt tgcggtgatc cctttttcag ccatcacccg acgatgacgc
     3121 aaaatcagtt ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc ggtgcataaa
     3181 gagcaggcgt agggtaaatc tttaaaatct ttatagccgc caagtaacgg ggaaatcacc
     3241 gcaccaattg gccctggata aatagagcca tatccatgac cgccaatatg gcgatatgcc
     3301 ggacaagtat tcatacaagc cccgcaacga atacagcgca gcacatcccg aaattcagag
     3361 gccagcacct cagaacgccc gttatcgaca ataaccagat gaaactcttc aggaccatca
     3421 acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa acgtgcacca
     3481 acggcactgc gcgccagcat ggtgatcaat acatctacct ctgcaaacgt gggagcaata
     3541 cgctccattc ccatcactgc aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta
     3601 ccttcattgg tcaccaggca taccgaaccg gtctctgcca ccgcgaaatt acagccggta
     3661 atacctattt cagcactgag gaaatcttcg cggatttttt gccgaatgaa taaggtcatc
     3721 gcttcaggcg tttccggccc ctcatagccc agacgttcgt gtagcactcg acggatctga
     3781 tggcgatctt tatgaattgc cgggaccaca acatgagagg gcggatcttg atccagctgg
     3841 agaatgtatt cacccagatc ggtttcaatc acctgaatcc cggcatcctg caacacatga
     3901 ttgacaccaa tctcttcggt caccatcgat ttagatttca ctaccttccg ggcatttttg
     3961 cgttgggcaa cctgtaaaat gtagcgggtg gcgtcttctt tggtttttgc aaaatagacg
     4021 tgaccgccgt tttgtgtcac tttttctgag agctggtaca gataagcgtc gagattactc
     4081 agaacatgat cacgtatctg ggcggcccga tcgcgccact cctcccagtg ccccaattca
     4141 tcgaccattt tttgccgatt tgccccaata cgctgctgcg cgtttgccac cgctttacgc
     4201 atgatcggat cttcaatttg ctgacggatg cgtgtcttaa aatctgtatt actggttttg
     4261 atcgacatct ttatatcctc agcggctcat cagcacttca gcaatatgca tcactttgac
     4321 tttctgccct tcccgttgta atcgtccact gatgttcagc aggcaactca cgtcagcacc
     4381 aattaaatac tcagggcgga cttccatcag gtgcgcaacc ttttctttca ccatctcgcc
     4441 ggatatttcg gccattttga ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc
     4501 agcaaaggtc aacagctcca gtccacgcac atttttcagc agcgtaagtg gttcgtcctt
     4561 cacccccagc ttacgggcca ggctacaaga tgggtgatac accgctctcc cttgcaaact
     4621 ggcacctaca tcgactaccc ctaatgtatt aacaataaaa gaggtgagat cctgcatacg
     4681 cgcggcaacc ttttcggcac gtgatgccca ttcaggttca tccgccagat acgtcgggta
     4741 actttttacg gcataggtgc aagagccagc cggtgaaata atgggatcgt cgttatcctc
     4801 cagtgcggcg atcagatttt tcatccctgg aatcgcttct ttgatataac cactattgat
     4861 cgcaggctga ccgcagcatc cctgtttctc cgggaaattt acgcgacagc cgagtttttc
     4921 cagtagcagc acggagtctc gtgccattct tgatttcagg gcgtcaccaa tacaggtgac
     4981 aaagaaattg acattcacaa ctaatactcc attacttcat gcccatttat gcccgatggt
     5041 tccccccttc cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca
     5101 acataccacc cctcatttgt atgacaaatg ataatctttc atctaaactt caaatgcaat
     5161 aactaatttt atttctttcg gcatcgctca cattttatag ggaaatcgac gatcattttt
     5221 ggcataagaa atccatttaa aaatggctac atcccttata cctattcaga gctacttatt
     5281 cagggattat tgagatatac actgatggtg tacaaaaaaa atcaccgaca ataatcacgt
     5341 atcaagtaat agtcgggatt ttatttaatt gaagaaattt agtttgcatt tttatttaaa
     5401 aactgagcta gagtccatca aaaaaatata taaaacccaa aaccaccgtt ggaaatagaa
     5461 atagttgaaa ggctcacaac tcccggaatg cggattatct ttgccctgat ttctggcggt
     5521 tttattcagg gtgcaagctg tctcaccctt tcccgatatt ctcccggagt acaaccaaac
     5581 tcgcggacaa acgccttgtg aaaagatgat tcgctggcat agcctactga ctcagcgatc
     5641 accacaacag ggagcatttc ccgggaaaac atctgggccg ctatttgtag acgcaacttt
     5701 gttaatacag ccagcggcgt ggttccggaa acatcacgga aaagctgggc aaaacttgcc
     5761 cgggacatgt gggcgatgct ggccagcgat tcgacggtcc aggcgtgtcc aggcatttcc
     5821 atcatttgct gtattaccgc accaagacgt ggatgcagaa gcaaactgag aatgtttttc
     5881 tctgtattaa cctgtgcaat ccattcacgc accgcgaggg taaagaatgt agcgcagatc
     5941 tggctacaca gagcatccac tcccggcatt gccgatctgg attcctgttg taaaaacggg
     6001 atcgcctccc tcagccagtt atattccacg ctgtggttaa ccggtgctac aaacagcgtt
     6061 tccggcaaag acgttaaaaa ataacgcgcc gaatgttgca gccgaagagt tccacagaca
     6121 atacaggtcg attcattatc gacatgactc aggcgatgag ctgaatttta tggtagcagg
     6181 accacatttc ccgggcgtaa tgtaaaaatc tctcccgtcg gcatttccag cttcgccgct
     6241 ccttgcgtta acgcatgcca acgaataacc gacaattccc cggcaccatg tggaagttgc
     6301 cagtcacttc ctaacacgca attcttatcg atcgttcctt gtggagcgtt aagcatcaac
     6361 aaacggctaa gagcatccat atcagactcc tgagtggttt tttgattaca aacaccaaca
     6421 atcttagtca ataattaatc taataccatc aaatagttaa tcatgatttt tgttttgtga
     6481 ttctgattaa gaaaaagaaa ataccagacg ataagacaaa atatcaagcg ttgtgaagaa
     6541 atgttatttg ctcttttgcg tctatagtca tgatgtcaaa tgaacgcgtt tcgacaggaa
     6601 atcatcatga ataaatatca ggcagttatt attggttttg gcaaggctgg aaaaacatta
     6661 gccgtcacgc tggcaaaagc aggttggcgt gtggctctca tcgaacaatc aaatgcaatg
     6721 tatggcggga cctgtattaa tatcggctgt atcccaacca aaacattggt tcatgacgca
     6781 cagcagcaca cagattttgt ccgtgccata cagcgtaaaa atgaagtggt taatttttta
     6841 cgtaataaga attttcataa tcttgcggat atgcccaata tcgacgtgat cgacggccag
     6901 gcggagttta tcaataatca tagcctgcgt gttcatcggc ctgggggaaa tctggagatt
     6961 catggcgaga aaatttttat taataccggt gcacaagccg tggttccgcc aattcctgga
     7021 attaccacca cgcctggtgt atatgacagc accggattac ttaatctaaa a
//
LOCUS       DAZGJT010000170         7065 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZGJT010000170 DAZGJT010000000
VERSION     DAZGJT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7065
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.173"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            571..1560
                     /gene="araF"
                     /locus_tag="ACHKFK_004523"
     CDS             571..1560
                     /gene="araF"
                     /locus_tag="ACHKFK_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO9054705.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITSNELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKASDIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKGVEPTKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1630..3144
                     /gene="araG"
                     /locus_tag="ACHKFK_004524"
     CDS             1630..3144
                     /gene="araG"
                     /locus_tag="ACHKFK_004524"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO9054706.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQIKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGMFGGTQITAGQVYIDQQPIDIRKPSHAITAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3159..4145
                     /gene="araH"
                     /locus_tag="ACHKFK_004525"
     CDS             3159..4145
                     /gene="araH"
                     /locus_tag="ACHKFK_004525"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO9054707.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4312..5112
                     /gene="otsB"
                     /locus_tag="ACHKFK_004526"
     CDS             4312..5112
                     /gene="otsB"
                     /locus_tag="ACHKFK_004526"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO9054708.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDTILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPKGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5087..6511
                     /gene="otsA"
                     /locus_tag="ACHKFK_004527"
     CDS             5087..6511
                     /gene="otsA"
                     /locus_tag="ACHKFK_004527"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO9054709.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNKFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTACGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6518..6946)
                     /gene="uspC"
                     /locus_tag="ACHKFK_004528"
     CDS             complement(6518..6946)
                     /gene="uspC"
                     /locus_tag="ACHKFK_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO9054710.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRNVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCRKHH
                     FDLVICGNHNHSFFSRASCSAKRVITSSEVDVLLVPLTGD"
BASE COUNT         1779 a         1639 c         1833 g         1814 t
ORIGIN      
        1 aattttaatg atggtgcttt ttgtatcttg ttgatattaa tcgatgaatt tgaattcatg
       61 atgaagcaat gattttataa aacgtttttt catttttgcg atatggctct cattatacgt
      121 gttctgttaa ttaatgagta cagtgataat taatggcgaa atgaatgacg cgcattttcc
      181 acatctttaa gttgcggtta ttacaccatt gcaaaaactc aacgccaggt aatgaggcct
      241 attgactggt taaaaagaaa acatcccgca tgggtaccaa agacaacaag gacttccagg
      301 ctaatcttat ggattattct gctgtgcact cgataatttg tctgacaaat tcgcttttcc
      361 ttatgtcttt tcccgctaaa tttatgcacg ttctcactgt aattctgcga tgtgatattg
      421 ctctcctatg gagaattaat ttctcgctaa aactatgtca acacagtcac ttatctttta
      481 gttaaaaggt aatgctttgt tttccgatta atttaacgaa tgtcattcgt ttttgcccta
      541 cacaaaacga cactaaagct ggagagaacc atgcacaaat ttactaaagc cctggcagcc
      601 attggtctgg cagccgttat gtcacaatcc gctatggcgg agaacctgaa gctcggtttc
      661 ctggtgaagc aaccggaaga gccgtggttc cagaccgaat ggaagtttgc cgataaagcc
      721 gggaaggatt tagggtttga agttattaag attgccgtac cggatggcga aaaaacattg
      781 aacgctatcg acagcctggc tgccagtggc gcgaaaggtt tcgttatttg tactccggac
      841 ccgaaactcg gctctgccat cgtggcgaaa gcgcgtggct acgatatgaa agtcattgcc
      901 gtggatgacc agtttgttaa cgcaaaaggt aagccaatgg ataccgttcc gctggtgatg
      961 atggctgcga ccaaaattgg cgaacgtcag ggccaggaac tgtataaaga gatgcagaaa
     1021 cgtggctggg atgtcaaaga aagcgcggtg atggcgatta cctccaacga actggatacg
     1081 gctcgtcgcc gtactacggg gtctatggat gcgctgaaag ctgcaggatt cccggaaaaa
     1141 caaatttatc aggtacctac caaatctaac gacatcccgg gggcatttga cgctgccaac
     1201 tcaatgctgg ttcaacatcc ggaagttaaa cattggctga ttgtcggtat gaacgacagc
     1261 accgtgctgg gcggcgtacg cgcgacggaa ggtcagggct ttaaagcgag cgatatcatc
     1321 ggtatcggta ttaacggtgt ggatgcggtg agtgaactgt ctaaggcaca ggcaaccggc
     1381 ttctacggtt ccctgctgcc aagcccggac gtacatggtt ataagtccag cgaaatgctt
     1441 tacaactggg ttgcaaaagg tgttgaaccg accaaattca ccgaagttac cgacgtggtg
     1501 ctgatcacgc gtgacaactt taaagaagaa ctggagaaaa aaggtttagg cggtaagtaa
     1561 tttgccggaa aaattcccct ctgcatgatg cagagggggt gtgaacgacc agtgattcac
     1621 ggagacgtta tgcaacagtc taccccgtat ctctcatttc gcggcatcgg taaaacgttt
     1681 cccggcgtta aggcgctgac ggatattagt tttgactgct atgccggtca ggttcatgcg
     1741 ttgatgggtg aaaatggcgc aggaaaatca actctcttaa aaatcctcag cggaaactat
     1801 gcgccaacca cgggttctgt agtgattaat gggcaggaaa tgtccttttc cgacacgacc
     1861 gcagcactta acgcgggggt ggcgattatt taccaggaac tgcatctcgt gccggaaatg
     1921 accgtcgcgg aaaacatcta tctcggccag ctgccgcata aaggcggcat tgtgaatcgt
     1981 tcattgctga attatgaggc aggtttacaa attaaacatc ttggtatgga tattgacccg
     2041 gacacgccgc tgaaatatct ctccattggt cagtggcaga tggttgaaat cgccaaagcg
     2101 ctggcgcgta acgccaaaat tatcgccttt gatgagccaa ccagctccct ctctgcccgc
     2161 gaaatcgaca atcttttccg cgttattcgt gaactgcgaa aagaggggcg ggtgatctta
     2221 tacgtttctc accgtatgga agaaatattt gccctcagcg atgccatcac cgtctttaaa
     2281 gatgggcgtt atgtcaaaac ctttaccgat atgcagcagg ttgaccacga cgcactggtg
     2341 caggcgatgg tcgggcgaga cattggcgat atctacggct ggcaaccgcg tagttatggc
     2401 gaggagcggc tgcgtcttga tgctgtgaaa gcaccaggcg tgcgtacgcc aataagtctg
     2461 gcggttcgca gtggagaaat tgtcggtctg tttggtctgg tcggggcagg gcgtagcgaa
     2521 ttaatgaaag gcatgtttgg cggtacgcaa atcaccgccg gtcaggttta tatcgaccaa
     2581 cagccgatcg atattcgtaa accgagccat gctattaccg caggcatgat gctctgcccg
     2641 gaagatcgca aagcggaagg cattattcct gtgcactccg ttcgcgacaa tatcaacatc
     2701 agtgccagac gcaaacatgt gctcggcggt tgtgtaatca acaacggttg ggaagaaaac
     2761 aatgccgatc accacattcg ttcgctcaac atcaaaacgc cgggcgctga gcaactgatt
     2821 atgaatctct caggtggaaa tcagcaaaaa gccattctgg gccgctggtt atcggaagag
     2881 atgaaggtca ttttgctgga tgaacctacg cgcggcattg atgttggtgc taagcacgaa
     2941 atatataacg tgatttatgc gctggcggcg cagggtgtgg cggtgctgtt tgcctccagc
     3001 gacttacctg aagtcctcgg cgttgccgac cggattgtgg tgatgcgcga aggtgaaatc
     3061 gccggtgaat tgttacacga gcaggcagat gagcgtcagg cactgagcct tgcgatgcct
     3121 aaagtcagcc aggcagttgc ctgagtaagg agagtatgat gtcttctgtt tctacatcgg
     3181 gttctggcgc acctaagtcg tcattcagct tcgggcgtat ctgggatcag tacggcatgc
     3241 tggtggtgtt tgcggtgctt tttatcgcct gtgccatttt tgtcccaaat tttgccacct
     3301 tcattaatat gaaagggttg ggcctggcaa tttccatgtc ggggatggtg gcttgtggca
     3361 tgttgttctg ccttgcttcc ggtgactttg acctttctgt cgcctccgta attgcctgtg
     3421 cgggtgtcac cacggcggtg gttattaatc tgactgaaag cctgtggatt ggcgtggcag
     3481 cggggttgct gctgggcatt ctctgtggtc tggtcaatgg ctttgttatc gccaaactca
     3541 aaataaatgc cctgatcacg acactggcaa cgatgcagat tgttcgaggt ctggcgtaca
     3601 tcatttcaga cggtaaagcg gtcggtatcg aagatgaaag cttctttgcc cttggttacg
     3661 ccaactggtt tggtctgcct gcgccaatct ggctcaccgt cgcgtgtctg atcatctttg
     3721 gcttgctgct gaataaaacc acctttgggc gtaacaccct ggcgattggc gggaacgaag
     3781 aggctgcgcg tctggcgggt gtaccggttg ttcgcaccaa aattattatc tttgttctct
     3841 ctggcctggt atcagcgata gccggaatta ttctggcttc acgtatgacc agtgggcagc
     3901 caatgacgtc gattggctat gagctgattg tgatctccgc ctgcgtttta ggtggcgttt
     3961 ctctgaaagg cggcatcgga aaaatctcat atgtggtggc gggtatctta attttaggca
     4021 ccgtggaaaa cgccatgaac ctgcttaata tttctccttt cgcacagtac gtagttcgcg
     4081 gcttaatcct gctggcagcg gtgattttcg accgttacaa gcaaaaagcg aaacgcactg
     4141 tctgatgctt ttttccacaa caatttagcg ttttttccca ccacagccag ccgccacaac
     4201 ggttggctgt tcttcgttgc aaatggcgac ccccgtcaca ctgtctatac ttacatgtct
     4261 gtaaagcgcg ttctgcgcaa cacaataaga aaagagaagg aggagaaccg ggtgacagaa
     4321 ccgttaactg aaacccctga actatccgcg aaatatgcct ggttttttga tctcgatgga
     4381 acgctggcgg aaatcaaacc gcatcccgat caggtcgtcg tgcctgacac tattctgcaa
     4441 ggacttcagc tactggcaac cgcaagtgat ggtgcattgg cattgatttc agggcgctca
     4501 atggtggagc ttgacgcact ggcaaaacct tatcgcttcc cgttagcggg cgtgcatggt
     4561 gcggagcgcc gtgacatcaa tggtaaaacg catatcgttc atctgccgga tgcgattgcg
     4621 cgtgatatta gcgtgcaact gcatacggtc atcgcccagt atcccggcgc ggagctggag
     4681 gcgaaaggga tggcttttgc gctgcattat cgccaggctc cgcagcatga agacgcattg
     4741 atgacattag cgcaacgtat tactcagatc tggccacaaa tggcgttaca gcagggaaag
     4801 tgtgttgtcg agatcaaacc gaaaggtacc agtaaaggtg aggcgattgc tgcctttatg
     4861 caggaagctc cctttatcgg gcgaacgccc gtatttctgg gcgatgatct gaccgatgaa
     4921 tctggcttcg cagtcgttaa ccggctgggc ggaatgtcag taaaaattgg cacaggtgca
     4981 actcaggcat catggcgact ggcgggcgtg ccggatgtct ggagctggct tgaaatgata
     5041 accaccgcat tacaacaaaa aagagaaaat aacaggagtg atgactatga gtcgtttagt
     5101 cgtagtatct aaccggattg caccaccaga cgagcacgcc gccagtgccg gtggccttgc
     5161 cgttggcata ctgggggcac tgaaagccgc aggtggactg tggtttggct ggagcggtga
     5221 aacagggaat gaggatcagc cgctaaaaaa ggtgaaaaag ggtaacatta cgtgggcctc
     5281 ttttaacctc agcgaacagg accttgacga atactacaac aaattctcca atgccgttct
     5341 ctggcccgct tttcattatc ggctcgatct ggtgcaattt cagcgtcctg cctgggatgg
     5401 ctatctacgc gtaaatgcgt tgctggcaga taaattactg ccgctgttgc aagacgatga
     5461 cattatctgg atccacgatt atcacctgtt gccctttgcg catgaattac gcaaacgggg
     5521 cgtgaataat cgcattggtt tctttctgca tattcctttc ccgacaccgg aaatcttcaa
     5581 cgcgctgccg acatatgaca ccttgcttga acagctttgt gattatgatt tgctgggttt
     5641 ccagacggag aacgatcgtc tggcgttcct ggattgcctt tctaacctga cccgcgtcac
     5701 gacacgtagc gcaaaaagcc atacggcctg cggcaaagcg tttcgaacgg aagtttaccc
     5761 gatcggcatt gagccgaaag aaatagcgaa acaggctgcc ggtccactgc cgccaaaact
     5821 ggcgcaactc aaagcagaac tgaaaaacgt acaaaatatc ttttctgtcg aacgactgga
     5881 ttattccaaa ggtttgccgg agcgttttct cgcctatgaa gcgttactgg aaaaatatcc
     5941 gcaacatcac ggtaaaattc gttataccca gatcgcgcca acgtctcgtg gtgatgtgca
     6001 agcctatcag gatattcgtc atcagctcga aaatgaagcc ggacgaatta atggtaaata
     6061 tgggcaatta ggctggacgc cgctttatta tttgaatcag cattttgacc gtaaattact
     6121 gatgaaaata ttccgctact ctgacgtggg cttagtgacg ccactgcgtg acgggatgaa
     6181 cctggttgca aaagagtatg ttgccgctca ggacccagcc aacccgggcg ttcttgttct
     6241 ttcgcaattt gcgggagcgg caaacgagtt aacgtcggcg ttaattgtta atccctacga
     6301 tcgtgacgaa gttgcagctg cgctggatcg tgcattgact atgtcgctgg cggaacgtat
     6361 ttcccgtcat gcagaaatgc tggacgttat cgtgaaaaac gatattaacc actggcagga
     6421 gtgcttcatt agcgacctaa agcagatagt tccgcgaagc gcggaaagcc agcagcgcga
     6481 taaagttgct acctttccta agcttgcgta ggtgcggtta atctcccgta agtggaacta
     6541 acagcacatc gacctcactt gaggtaatca ctcttttcgc cgagcaggat gctcgcgaaa
     6601 agaaactgtg attgtgatta ccgcaaatca ccaaatcgaa atgatgtttg cgacaaactt
     6661 ccagaatatg ttcgcttaac tcaccgtagg caataaacgt cttgtcaacg ggataccccg
     6721 catcctgaat taacttatca agaaagcttt gcgtttcttc ctgcatcaca ttgcgtaaat
     6781 cttcaagcat cggcgcagct aattgattgt acatttccgg gtcggaagcg agagtaatta
     6841 aactgatgtg tccctttact ggcctggcga tagataccgc ttttgccagc agttgctgac
     6901 tttccggtgt aaccgcaacg gcgacaagaa tattgctata gctcataacc tgttccttat
     6961 tctgtgaact tcaggtgaca ttaaagcttt actcctaatg gagaaacgac gcaatcccaa
     7021 ctcagttaaa caggtaaata ttagtctggt cattagtaaa tacaa
//
LOCUS       DAZGJT010000171         6997 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZGJT010000171 DAZGJT010000000
VERSION     DAZGJT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.174"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            57..1250
                     /locus_tag="ACHKFK_004529"
     CDS             57..1250
                     /locus_tag="ACHKFK_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO9054711.1"
                     /translation="MSSKIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLM
                     ATGAVTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVV
                     LRPQRLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWH
                     YRTIK"
     gene            complement(1630..2010)
                     /gene="crcB"
                     /locus_tag="ACHKFK_004530"
     CDS             complement(1630..2010)
                     /gene="crcB"
                     /locus_tag="ACHKFK_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO9054712.1"
                     /translation="MIKSLFAVIIGGSVGCTLRWLLSTRFNSLFPNLPPGTLVVNLLA
                     GLIIGTALAYFLRQPHLDPFWKLMITTGLCGGLSTFSTFSVEVFALLQAGNYIWALTS
                     VLVHVIGSLIMTALGFFIITILFA"
     gene            complement(2351..2764)
                     /locus_tag="ACHKFK_004531"
                     /pseudo
     CDS             complement(2351..2764)
                     /locus_tag="ACHKFK_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015377870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
     gene            2762..3019
                     /locus_tag="ACHKFK_004532"
                     /pseudo
     CDS             2762..3019
                     /locus_tag="ACHKFK_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(3253..4110)
                     /gene="sitD"
                     /locus_tag="ACHKFK_004533"
     CDS             complement(3253..4110)
                     /gene="sitD"
                     /locus_tag="ACHKFK_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HFO9054713.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKSWALMG
                     DAMSHAVFPGIVLAWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAVKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQASGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISILVSMTGVWLSFFIDSAPAPTIVVLFAVMFIMTFTVTSINARTKENA
                     EPRDLLSSD"
     gene            complement(4107..4964)
                     /gene="sitC"
                     /locus_tag="ACHKFK_004534"
     CDS             complement(4107..4964)
                     /gene="sitC"
                     /locus_tag="ACHKFK_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HFO9054714.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQLTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLMIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(4961..5788)
                     /gene="sitB"
                     /locus_tag="ACHKFK_004535"
     CDS             complement(4961..5788)
                     /gene="sitB"
                     /locus_tag="ACHKFK_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HFO9054715.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPGGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREER"
     gene            complement(5788..6702)
                     /gene="sitA"
                     /locus_tag="ACHKFK_004536"
     CDS             complement(5788..6702)
                     /gene="sitA"
                     /locus_tag="ACHKFK_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HFO9054716.1"
                     /translation="MLSIKKVTMLLGCLVLTCSIAFQASAAEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDAFIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQIAELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1868 a         1725 c         1692 g         1712 t
ORIGIN      
        1 cagaaaaatt aattttacaa ataaacacaa ttaatacatg gtgttgaaac attacaatgt
       61 catctaagat cgaagatacg ccccaaaaaa ccctgtcctg ctggccactg gcgttcagtg
      121 ccggtcttct cggtatcgga cagaacggtc tgctggttgt actccctgtt ctggtcatac
      181 agacaaatct gagtctgtct gtatgggctg ccctgctgat gctgggctca atgctgtttc
      241 tgccatcttc cccatggtgg ggaaagcaaa tttcccttac tggcagtaag actgtggtgc
      301 tgtgggctct gggaggatat ggcgtaagct ttaccctgct tgggctggga agcgtgctga
      361 tggctaccgg tgccgtaaca acagcggtgg ggttgggaat attaatcatc gcccggatcg
      421 tctacggtct gaccgtgtca gcaatggtgc cagcctgtca ggtctgggca ttgcagagag
      481 cgggagaagg gaatcgcatg gccgctctgg caaccatcag ctccggcctg agctgcggca
      541 ggctattcgg gccgctgtgc gcggcggcaa tgttggtcat tcaccctctg gcgccagtgt
      601 ggatgctgat ggcagctccg gtgtgggctg tggtgatgct tctgcggttg cccggcacac
      661 caccacagcc cacaccggag cgcaagagcg tcagcctgaa gcgggattgc ctgccttatc
      721 tgctttgcgc aatgttactg gctgcggcaa tgagcatgat gcagcttgga ctttcgccag
      781 cccttactcg ccagttcgat actgatacca ccactattag ccaacaggtg gcgtggttgt
      841 tggggctgtc cgcaatagct gcgcttatcg cgcagttcgt ggtactccgt ccacagcgcc
      901 tgactccagt ggctctgctc ctgagtgccg gggtgttgat gagtagtggt ctggctatca
      961 tgctcactga acagctatgg ttgttttacc tgggctgtgc agtgctgtca tttggggccg
     1021 ctctggcaac acccgcttat caacttttac tgaatgataa gctggccgac ggcgcaggcg
     1081 cgggctggct tgcggccagt cacacacttg gctatgggct gtgcgcattg ttggtgccac
     1141 tggcgtcgaa aacaggtgtc tcaatcgcgc tgattgtgac ggcattattt gccgctgtat
     1201 tatttaccat agtgtctgca tgtatttggc attaccgtac aatcaagtaa ggctatgatg
     1261 ttgtactcac agccggcata acgtcctgtg caatttcgct gtcgaaaccg cctgtgctga
     1321 ccgcccagtg cactcacctg aagcacatcg cagcatgcca ccaggatgcc agatcgtctt
     1381 ctgaaatggg tggatacatt ggcaacgtta accgcaacaa tttttcagag agaattttgg
     1441 ggggtgaggg ccagtgatgt aaaaaatacg ccaagaggtt aattcattgt ttatactgat
     1501 atgtcaggta atgcgtttgc agtcattaca gtcgtcatgg agactgtaat gactgcaaac
     1561 gcataccatg gagcgaaatt ctatcctaag cgtcacatta gttgataaac ctgtaacgtt
     1621 aatgaattct catgcgaaaa gtatggttat gataaaaaag cctaaagctg tcatgatgag
     1681 ggagccaatg acgtgaacaa gtacagacgt caaagcccat atataatttc ccgcctggag
     1741 taatgcgaat acttcaacag aaaatgtaga gaaagtactc agacctccac aaagtcccgt
     1801 tgttatcatt aatttccaaa aaggatcaag gtgtggttgt cgtaaaaagt acgccaacgc
     1861 agtaccaata attaacccgg caaggaggtt caccaccagt gtacctggag gtagatttgg
     1921 aaaaagactg ttaaagcgtg tggacagtaa ccagcgcaga gtacatccaa cagaaccgcc
     1981 gataatgacg gcaaaaagtg atttaatcat gtacccctca taaaataatt ttttatcgta
     2041 acaatatcag aatatgttct catttgactg atattgttaa tttatatcct ttgactacat
     2101 gcaagataag ggaaggttct atttattgac attaataccc tcgacaattg ctgagcagaa
     2161 gcccccacga caagtgacag aatgacaaca tctacagtgg ggagcgtttt atcgatgaac
     2221 aagcaaccaa gaagtaacac ggcataggtg acgacaatgc cagtaagcat agaaataaca
     2281 aaaacgtgag ctttcatcat tcctccagaa taaccccgac gtgtaagcct gggggattgg
     2341 cacaataatg tcatttaaag gcagactttc cggcaaattg cgccgatgaa cccagttcat
     2401 cctcaattct cataagctgg ttatattttt caatacgctc accccggcat ggagctcccg
     2461 ttttgagatg acctgcgcgc attgcaactg tcatatcggc gataaagctg tccactgtct
     2521 caccactgcg gtgtgagata aaggtgcccc agttgttgtc atggcaaagt tgtaccgcat
     2581 caaaggtttc actcagggaa ccaatctggt tgagtttaat caatgctgaa ttggcaaggt
     2641 tttcgtcaat gccgcgctga atatacttta cgttggtcac aaacagatcg tcgccaacca
     2701 attctatctg ctctcccaat gcaccatgca ggattttcca tccagcccag tcatcttcag
     2761 ctaccgttaa gggcaggaag atcaactagg tgctctgggg ctggtgacca atgcggttgt
     2821 gctgtgaaac acactttaca tgcaggaggc gttgtcacat cttcgcaaat cgggagaaac
     2881 tcctgaggag tagcatccgg caagattatc gctgctgata catagccata taaatatgct
     2941 ggggcattat acgtcttcgt taccggaaga tattcttaag ggtgagttaa gatcattaaa
     3001 cttcaataca aacaattaat taaactctta gcgtggtttt ttacatcact ggccctcgcc
     3061 ccccaatttt ctctatgttt tgccactact tccctcattt tactgcgctg tttgctaccc
     3121 ctgagaatcg acattacttg ctatccagtc cgcagcaact ctctggcaac aaaacttatc
     3181 agaatcataa gtctgtgcat tattcgttca agcgcatcgc caataagcgg atgttgctgg
     3241 ccgagtgttc tgctaatcgg atgataacaa atcccgtggt tcggcatttt cctttgtgcg
     3301 tgcgttgatg ctggtaacgg taaacgtcat gatgaacatg accgcgaata agacaacaat
     3361 tgtcggagct ggcgcgctgt cgataaaaaa ggacagccag acaccagtca ttgagaccaa
     3421 tatcgatatg ccagtcgcca gcaggagcgc aatatgaaag cgttgtgtca ggagtaccgc
     3481 aatggcacca ggcgcaatga gaagagaaat agagagaatg atacctaccg ctttcagcgt
     3541 cgccacaatg gtcagggaga ccatgcacag cagcccataa tgcagccatc tcgtatgcaa
     3601 accgcttgcc tgcgcctgtt gataatcaaa gctgaacagc aaaaaatccc gccattttac
     3661 gctaataaca agtgtaacca gtccggcaat aatcaccgtc tggattatat cgccgatggt
     3721 tatcccgagc atgtcgccga aaagaatatg atcaagatgt acgtctggtt tgactgcaat
     3781 atacaagatc agacctgcgg caaacatgcc agaaaaaaca atccccatca ctgtatcttg
     3841 cttgattcgg ctgttgtctt tcaggtatcc cgtcgccacc gcacagaata ctccggcgac
     3901 gaatgcccct gtagccagag gtaaccccag aatccaggca agcacgatgc caggaaaaac
     3961 cgcatgactc atcgcgtcac ccatcagtgc ccaacttttc aggaccagaa agaccgacag
     4021 cagcgcacag ggtatggaaa ctaccagtga aatcagcagt gcgtgactca taaaggtgaa
     4081 ctgcagtggc tccagtaata atgccatcat gagcgatcct ccagcgcttt atgcgcacgc
     4141 cgacgatttg ccaaaagccc atgggtgggc gcaaagacaa atgctaaaag aaacagcagt
     4201 gtctgcgcca ccacgatgat tccacctgtc gcgccgtcca gaaagtaact gacccacgcg
     4261 ccgaggaaac tggtcacact gccaatagtt acagcaatca tgagcaggcg gggaaaacga
     4321 tcggttaaca accatgctgt tgcaccggga gtcaccacca gacagatcac cagaaaggca
     4381 ccaaccgttt gcagcgccgc aacggtcgaa accgacagca gggtgaagaa gaggattttt
     4441 aatctgcccg gatgcaatcc aatagcacgg gcgtgattct cgtcgaaaaa ggtcaccata
     4501 agatctttcc atttgaaaaa aagcacgatg atcgagagaa tgccaatgat agtgagttgc
     4561 aggatatccg ccggatcgat tgccagaata ttgcccagga cgatggtctg aatgttcacg
     4621 gatgtcgggt tcaatgacac cataaacagc cccaggccaa aaaaggacga gaagatcagg
     4681 ccgatgatgg catcttcttt taagcgagtg agttggttaa gaaacagcat gctgcccgct
     4741 gccagtccgc cggaaaagaa ggctcccaga gaaaacggaa gccccagcat atatgccccc
     4801 gcaacacccg ggacaataga gtgcgacagc gcatcgccaa tcagcgacca gccttttagc
     4861 atcagatagc atgacaggaa agcgcagaga ccgccgacca tcgccgagac ccacatcgca
     4921 ttgagcatat attcatagct gaagggttcc agtagcacgt tcatctttct tccctctgca
     4981 ccgctgacgg tcgatgtgcc acaaaagggc gttcatcgtc agtaataatg ctttcttccg
     5041 agccgttaag tgtgacatgg cgcagtacgc cgctaaaagc cagctccagg ttctcggctg
     5101 taaaagtggt gtctgtcggc ccgcttgcca ggacggtgcc tttgaccatg accgtataat
     5161 cgcaaaatgt cgtgacggat ccgagattgt gcgttgagac aagcatcgtt ttgccttccg
     5221 cgcgtaactc cctcaacagg ctgatgattt tcgcttcggt tttcacatca acgccggtaa
     5281 aaggttcatc cagcaggatc acatcaccct gctgtgcaat cgctctcgcc agaaagacgc
     5341 gtttcttttg tccaccggaa agctcgccga tttgtcgatg gcgaaaatcc accatatcga
     5401 cacgttccag cgcatcggtg acaatctgac gatccctctt tttggcgata cgcaacatac
     5461 ccatatgccc atagcggccc atcatcacca catcttccac cagtacagga aatgaccagt
     5521 caacctcttc tgactgaggg acgtaggcaa ccaggttttt ctgtagcgcc tgtcgtgtgg
     5581 gaatacccag aacagatatc ttcccgctgg tcagacgcac aaatcccata atcgctttaa
     5641 acaacgtcga ttttccggaa ccgtttaccc caaccagagc ggcaatagat ccgcctggta
     5701 ctgtgaagga tgcatcgcgc agtgctgtgt gcccgttacg ccaggtgaca gtaacatcat
     5761 taacgacaat gcctgcagat tgcatcatta tttctccctt tttccggctt taattccctg
     5821 taccagcgta ctggtagtaa ccttcagaag gtcgatatac gtaggtaccg ggccgttttc
     5881 tgtgctcagg gaatcgacat agagcacacc accgtagtgc gcgccggttt cacgcgcaac
     5941 ctgacgcgct ggtttatcgg aaatcgtact ctcgctaaag actgccggga tatgattttt
     6001 cttaactata tcaacaacct tacgtacctg ctgcggtgtt ccttgttgat cggcattaat
     6061 cggccacaga taaagctctt ttagccccaa atcgcgtgcg agataagaaa aagccccttc
     6121 actggtgacc atccatcgct gattctcagg gagttccgca atctgcttac gcaggggggc
     6181 aagggtttgg gtaatcttgg ctttataagt atcggcatta cgttggtagg tttgtgcatt
     6241 tgccggatcg tattttataa acgcatcacg aatattatcg acgtaaatca gagcattatc
     6301 tggcgacatc caggcatggg ggttaggttt gccctcatag ggcccttcgg tgatccctac
     6361 tggcgtcaca cccgaagaga caattacttc tggaacccca ttgagatgct ggtaaaagcg
     6421 ttggaaccac aattccagat tcataccatt ggcgagaatc agttgtgccc cctgcgcacg
     6481 tttaatatcg ccaggggtag gctgatactc atgaatttct gcaccaggct tggttatgga
     6541 tgagacttct gcagcatctc cagccacgtt tttggccata tctgcgatga tggtaaaagt
     6601 tgtaatgacc ttgaattttt cagctgcact cgcctgaaat gctatcgagc aggtgaggac
     6661 taggcacccc aagagcatgg ttactttttt tattgagagc atgatttagt acctatagtg
     6721 agcaaaatta gaataaacat agcaactact atgttttata gcagaagcta taaaatgtaa
     6781 ataattatca ttagcgatta taagatccca acacagggat taggttattt cctgctgacg
     6841 atgtggtatt gaggataaaa gttgggcagc gctttggctt taaaatgaac ttgttcagga
     6901 cgattggtaa tggcaaagca aaaataatgt aggttattgg caagttctct aggacgcagg
     6961 agagcatgaa aaacccaggg gcagtcacta aatgaag
//
LOCUS       DAZGJT010000172         6845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZGJT010000172 DAZGJT010000000
VERSION     DAZGJT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.175"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..1170
                     /gene="emrK"
                     /locus_tag="ACHKFK_004537"
     CDS             7..1170
                     /gene="emrK"
                     /locus_tag="ACHKFK_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO9054717.1"
                     /translation="MEQVNSNKKHSDRRKYFALLAVVLFIAFSGAYAYWSMELKDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKEALEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVIEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            1170..2708
                     /gene="emrY"
                     /locus_tag="ACHKFK_004538"
     CDS             1170..2708
                     /gene="emrY"
                     /locus_tag="ACHKFK_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO9054718.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2816..4144)
                     /gene="dsdA"
                     /locus_tag="ACHKFK_004539"
     CDS             complement(2816..4144)
                     /gene="dsdA"
                     /locus_tag="ACHKFK_004539"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO9054719.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAYAEKLALEAGLLTLEDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAEQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4162..5499)
                     /gene="dsdX"
                     /locus_tag="ACHKFK_004540"
     CDS             complement(4162..5499)
                     /gene="dsdX"
                     /locus_tag="ACHKFK_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HFO9054720.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYTLLEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            5717..6664
                     /gene="dsdC"
                     /locus_tag="ACHKFK_004541"
     CDS             5717..6664
                     /gene="dsdC"
                     /locus_tag="ACHKFK_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFO9054721.1"
                     /translation="MEPLREIRNRLLNGWQLSKLHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLAHHFLMDEDILPVCSPEYAQRHDLTNTVINLRHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVSQYECYTL"
BASE COUNT         1910 a         1542 c         1562 g         1831 t
ORIGIN      
        1 ataatagtgg aacaggttaa ttcaaataaa aaacattctg acagaagaaa gtattttgct
       61 ttgttagcgg tagttttatt tattgcgttt tcaggtgcct atgcctattg gtcaatggaa
      121 ttaaaagaca tgattagtac agatgacgcc tatgtcacgg gaaatgcaga tccaatttct
      181 gcacaagtct caggcagtgt cactgtcgtt aatcataaag atacgaacta cgttcgacaa
      241 ggtgacattt tagtttcact ggataaaact gatgccacta tcgcactcaa taaagctaaa
      301 aataatctgg caaatattgt tcggcaaacg aataaactat acttacagga taaacaatac
      361 agtgccgaag tcgcttcagc acgtattcag tatcaacaat ctttagaaga ttataaccgt
      421 cgtgtgcctt tagcgaagca aggggttatt tcaaaagaag cgctggagca taccaaagat
      481 acgttaataa gtagcaaagc ggcattgaat gctgctatcc aggcttataa agcgaataaa
      541 gctttagtaa tgaacacacc attaaaccgt cagccacaag tcattgaagc tgcagatgca
      601 accaaagaag cctggttggc gcttaaacgt acggatatta agagtccggt taccggttat
      661 attgcccaga gaagtgttca ggtcggcgaa acagtgagtc ccgggcaatc actaatggct
      721 gtcgtaccgg cgcgtcaaat gtgggttaat gccaacttta aagaaacaca actcacggat
      781 gtacggattg gtcaatcggt caatattatc agcgatcttt atggtgaaaa tgttgtgttt
      841 catggtcggg tgacagggat caatatggga accggcaatg cgttctcctt attacctgca
      901 caaaatgcga cagggaactg gatcaaaatc gttcagcgtg taccggttga agtttctctg
      961 gatccaaaag aactcatgga acaccctttg cgcattggtt tatcgatgac tgctactatt
     1021 gatacgaaga atgaagacat tgccgagatg cccgatctgg cttcaaccgt gacgtccatg
     1081 cctgcttata ccagtaaggc tttagttatc gataccagtc cgatagaaaa agaaattagc
     1141 aacattattt cgcataatgg acaactttaa tggcaatcac taaatcaact cctgcaccat
     1201 taaccggtgg gacgttatgg tgcgtcacta ttgcattgtc attagcgacg tttatgcaaa
     1261 tgttggattc cactatttct aacgtcgcaa taccgacaat atctggcttt ctgggagcat
     1321 caacagacga aggcacctgg gttatcacct cgtttggcgt agcaaatgcc attgcgatcc
     1381 ctgttaccgg caggctggca caaagaatag gcgaattaag gttattttta ctttcagtaa
     1441 cctttttttc gctgtcttca ttaatgtgta gtttatcgac caatcttgat gtgctgatat
     1501 tttttagagt cgttcagggg ttaatggcgg ggccgttaat tccactgtca cagagtttat
     1561 tattaaggaa ttatccacca gaaaaaagaa catttgctct ggcattatgg tcaatgaccg
     1621 tgattatcgc tccgatatgt gggccgatat tgggcggcta tatttgcgat aactttagct
     1681 ggggttggat atttttaatc aatgtcccta tggggattat cgtcctgaca ttatgcttaa
     1741 ccttacttaa aggacgagaa actgagactt caccggtcaa aatgaatcta ccaggactga
     1801 ccctgttagt gctcggtgtt ggtggcttgc aaattatgct tgataaaggg cgcgatctgg
     1861 attggttcaa ctcgagtaca ataataatac taacagtagt atcagttatt tctctgattt
     1921 ctttagtcat ttgggagtcg acctcagaga atccgattct tgacctcagt ttgtttaagt
     1981 cccgtaattt caccattggt attgtgagta tcacatgcgc gtatttattt tactctggag
     2041 cgatagttct tatgccgcag ttactccagg aaacgatggg gtataatgcg atatgggccg
     2101 gacttgccta tgcgcccatc ggtatcatgc cgctattaat atcacctttg ataggacgtt
     2161 atggcaacaa aattgatatg cggttgttgg tgacatttag ttttttgatg tatgcggttt
     2221 gctattactg gcgttctgtg acatttatgc caacgattga ttttacaggc atcattttgc
     2281 cacagttttt tcagggattc gccgttgcct gtttcttttt acccttaaca acgatttcgt
     2341 tttcaggctt gccagataat aaatttgcca atgcctcgag tatgagtaat ttttttcgca
     2401 ccttgtcagg atcagttggt acgtcgttga caatgacact gtggggacgc cgcgaatcgt
     2461 tacaccatag tcagttgaca gcaaccatcg atcaatttaa ccccgtgttt aattcatcgt
     2521 cacaaattat ggataaatac tatggttcgc tttcaggagt tcttaatgaa attaataatg
     2581 aaataactca gcagtcactt tcaatttctg caaatgagat tttccgtatg gcggctattg
     2641 cttttatctt acttacggta ttggtttggt ttgcgaaacc gccgtttaca gcgaaaggcg
     2701 ttgggtgatg gtataaagag agaattatag caggtaagac cggggatctt aagtctgcgc
     2761 catcggcagc atcgctcacc cagggaaagg atggcgatgc tgcattgaaa attaattagc
     2821 ggccttttgc cagatattga ttcatctctt cttccggcac cattccacct cccgtcgccc
     2881 acaccagatg agtggcatta cgcagttgtt ctgcgctgaa accgtgcatc tgttggtaac
     2941 ttactgatgc gcacacgcgc tgaggcccgg ccatacccgc cagtgccgaa ggttcaagac
     3001 gaataccttc ttcctgcgcc agccagccaa gcatgtcata catggtttga tcgctaaggg
     3061 tatagaagcc atccagtaga cgctccattg cccgcccgac aaagcctgat gcgcgaccaa
     3121 cagcaaggcc atccgctgcg gtaaggttgt cgataccaat atcctgaaca gagatctgat
     3181 cgtgtaatcc agtatggacg cctaacagca tacaagggga gtgcgttggt tcggcaaaaa
     3241 aacagtgaac atgatcgcca aacgccagtt taagtccgaa tgcgacgcca ccaggaccac
     3301 cgccaacacc acacggcaga tagacaaaca gagggttatc agcatcgacg atacgacctt
     3361 gctcggcaaa ttgcgcttta agacgctggc cagcgacgga atacccaagg aacaacgtgc
     3421 gggaattctc gtcatcaata aagaaacagt tcgggtcaga ctgcgctgct ttacgtcctt
     3481 cctcgacggc aacaccataa tcttgctcat attccacgac cgtaacgcca tggctgcgca
     3541 gtttcgcttt tttccatgcc cgggcatcag cagacatatg tactgtcacc ttaaagccaa
     3601 tgcgggcgct cataatgccg attgataacc ccagatttcc ggttgagccc acagcaatgc
     3661 tgtattggct aaagaactgt ttaaactccg gagaaagcag tttgctgtag tcatcttcaa
     3721 gcgtcagcaa ccccgcttcc agagccagtt tttctgcata tgccaggact tcataaatcc
     3781 cgccgcgtgc ttttatggag ccggaaatgg gcaaatggct atcttttttc agtagcagtt
     3841 gcccgctgat cggttgttga tattcctttt ccagccgttt ttgcatagcc ggaatggcaa
     3901 ccagttctga ttcaataatc cccccagtag cagcagtttc aggaaatgct tttgccagat
     3961 agggtgcaaa acgggataag cgcgcatggg cgtcctgaac atcctgttcg gtcaggccaa
     4021 cataaggtaa accttcagcc aatgaggtcg tgccaggatt aaaccaggtg gtttctttaa
     4081 gagcaaccag atcctttacc aacggatact gggcgatgag cgagttcatt ttagcgtttt
     4141 ccatagtacg tctctttgcg cttagatgat aaatgacagc aggaatgtgc ccgccagagc
     4201 gacgactgaa gcgataaatg tcgctgtcgt atagtattta aatgtttcat tgagcgtagc
     4261 gccgcaatat tgcttcacca gccagaaaag cgagtccgta acgatcgtgc agccaatcgc
     4321 acctgaaccg atagcaatcg caataatttc cggactgatg tcgggataca gcggcagcat
     4381 gggtgcaaca attgccgttg cccccatcat tgccaccgta gcggagccca ctgcagcatg
     4441 cagaataagt gccaccaacc aggccagaag aatcgggtgc atatgcatat tggagagaat
     4501 aactgccagc gtatcagcga gactgctgct ttttaaaatg gcgttgaatg cgcctccggc
     4561 cccgataatc agcaaaatat tagcaataga accgaaacca ttttccgtat gtgtgagcat
     4621 cgtccccatg ctcatatgct gacgtatacc caacacataa taggcgacaa acacggcgat
     4681 aaacatggca gtgatagggt tgccaataaa ctcaagcaag gtatacaaac cactctcacg
     4741 tgccatattc aattcggcaa tcgttttaac caacatcagc gcaatgggta gcagtatggt
     4801 gaataacgtt gcgcctaatg acggtagtgt tttttcatcg cgaactttga gatcggcaaa
     4861 ctctgtgggt acaggtttaa agggcagtcg ttgacccaga aatttaagga aaagtgggcc
     4921 accgatcagt gatgccatca gcccaaccag caaaccgtag acgatcaccg aaccgatatc
     4981 tgcgcccagc ttattggcaa catataaagc agccggatgt gggggaacca cgcagtgcac
     5041 tgccatcaat gcggtacaca gcggaatggc cagctttaac aatgaggtat tggttttttt
     5101 ggcaattgaa aaagccagag gaatcaatag cacgacgccc acttcaacaa acagcgtgat
     5161 gccgcaaatc aggccaacca gcaccataat gacatcagct gaaagccacc ggcagcgttg
     5221 aagtgtcaga ccaattcttt ctgcggcccc ggatacttcc atcatttttc ccagtatcgt
     5281 gccaaggccg ataaccgcag cgaggaaccc cagcgttccg ccaattccac tttcaatagc
     5341 atttaccata tccagtggcc ccatgcccat catcgttccc acgaagaagc tggccagcaa
     5401 cagcgccaga aacgggtgga atttgaactt cacgatggtc agtacaatta acacgatgct
     5461 gataagcagc gtgctcacaa cccagatttg agagtgcata tctcaccttc ccctgtgatt
     5521 aacctgatga tattggacaa aaaaacaacg caggctgaca aacgataaaa tttgcctttc
     5581 acatgagcta aattgcatca agagagtgat ttacatctaa aaatgaaggt gaattgagat
     5641 atggttcact ttagctcacc ttacagctga aattttgccg ctattttttt acacttaaga
     5701 gaaaaatgag gtgattatgg aaccccttcg tgaaataaga aatcgactgc ttaacggctg
     5761 gcaactatca aaactgcata cttttgaagt ggctgccagg catcagtcct tcgctctcgc
     5821 ggcagaggaa ttgtcgctga gccccagtgc ggtaagtcac cgtatcaatc agctggaaga
     5881 agaattaggt attcagttgt ttgttcgttc ccatcgcaaa gtggaattaa cgcacgaggg
     5941 gaaacgtgtt tattgggcgc taaaatcgtc gctggatacc ctgaatcagg aaattctgga
     6001 tatcaaaaat caggagttat cgggaacgtt aacgctgtat tcccggccct ctatcgccca
     6061 atgctggttg gtgcccgcat taggtgactt tacacgccga tatccgtcta tttcgctcac
     6121 cgtgctcact ggtaatgaca atgtcaattt gcaacgtgcc ggaatcgatt tggcgatcta
     6181 ctttgatgat gcgccgtcag cgcaattggc tcatcacttt ctgatggatg aagatatctt
     6241 gccagtttgc agcccggagt acgctcaaag acatgattta accaacacgg taattaacct
     6301 gcgtcactgt acgttgctcc atgacagaca ggcatggagc aacgactccg gtacggatga
     6361 atggcatagt tgggcgcaac attatgcggt taatttgcca acatcttctg gaattggctt
     6421 tgatcgctct gatttagccg ttatcgccgc gatgaatcat attggggtgg cgatgggaag
     6481 gaaacgtctg gtacaaaaaa ggcttgccag tggtgagctc gtcgcgccgt ttggcgatat
     6541 gacggtgaaa tgccatcagc attattacat caccacatta ccgggcaggc agtggccaaa
     6601 aattgaggca tttattactt ggttaagaga acaggtaagt caatatgaat gttatacctt
     6661 ataatacatt aaatcatctg gttcctaaat aaccttatta tgcactgtaa gtgtatgttg
     6721 gctttataaa ttgcaatttt tatatgggaa ttgttttgtc aatagttacg aaaaggataa
     6781 ataaaataac cacttcttct tggcgtcgaa tattggagag cgataattta tagtaaacta
     6841 gatta
//
LOCUS       DAZGJT010000173         6733 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZGJT010000173 DAZGJT010000000
VERSION     DAZGJT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.176"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            225..338
                     /gene="yhiY"
                     /locus_tag="ACHKFK_004542"
     CDS             225..338
                     /gene="yhiY"
                     /locus_tag="ACHKFK_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO9054722.1"
                     /translation="MMTTLLPVFTKPSPLALNALCAGRICHFLLIPDGRIR"
     gene            401..1468
                     /gene="yhiI"
                     /locus_tag="ACHKFK_004543"
     CDS             401..1468
                     /gene="yhiI"
                     /locus_tag="ACHKFK_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO9054723.1"
                     /translation="MDKSKRHLVWWGVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1465..4200
                     /gene="rbbA"
                     /locus_tag="ACHKFK_004544"
     CDS             1465..4200
                     /gene="rbbA"
                     /locus_tag="ACHKFK_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO9054724.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPPELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDISRGTPVKLGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCALSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4200..5324
                     /gene="yhhJ"
                     /locus_tag="ACHKFK_004545"
     CDS             4200..5324
                     /gene="yhhJ"
                     /locus_tag="ACHKFK_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9054725.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5397..5663
                     /locus_tag="ACHKFK_004546"
     CDS             5397..5663
                     /locus_tag="ACHKFK_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO9054726.1"
                     /translation="MRTQVQALRKKQKNTLDQIFKSPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLRKP"
     gene            5660..6019
                     /locus_tag="ACHKFK_004547"
     CDS             5660..6019
                     /locus_tag="ACHKFK_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO9054727.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6069..>6733)
                     /locus_tag="ACHKFK_004548"
     CDS             complement(6069..>6733)
                     /locus_tag="ACHKFK_004548"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO9054728.1"
                     /translation="KRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVMIDGAAFSF
                     EENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKTFVERTGCD
                     MLAVSIGNVHGLDDIPRIDIPLLKRIAEVSPVPLVIHGGSGIAPEILRSFVNYRVAKV
                     NIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMNEGHRLIRK
                     AS"
BASE COUNT         1528 a         1707 c         1896 g         1602 t
ORIGIN      
        1 aataacgcta tgtgatagtc atcaggtttc cctgttacgg aaacctgtgc aacagaatct
       61 gtgaacctgt acagaatatt tttggaaaaa acaccagcga acggaagaac atcgcgatgt
      121 atcacaagtt gatggcaatc aaatagacaa catgaatata actgttagta tttaattgat
      181 ttcacttatg agtatgcgtg tttcatttac ggccaggaat gctgatgatg acaacattac
      241 tacccgtttt cactaagcct tccccattgg cgctcaatgc tctgtgcgct ggtcgtattt
      301 gccatttcct tcttatcccg gatggaagaa tccgctgaac atcgttcggt ggcagttgta
      361 acacgcgtcg attgcttaca gggattatgg cggatcgatt atggataaga gtaagcgcca
      421 tctggtgtgg tggggtgtcg gggcactggc ggtggctgcc gtcgtggcgt ggtggctgtt
      481 gcgcccggcg ggtgtgccgg aaggctttgc tgtcagcaat gggcgcattg aagcgacgga
      541 agtggatatc gccagcaaaa ttgccgggcg tatcgacacc attctggtga aagaaggcca
      601 gtttgttcga gaaggtgaag tgctggcgaa gatggatact cgcgtgttgc aggaacagcg
      661 actggaagcc atcgcgcaaa tcaaagaagc acaaagtgcc gttgccgccg cgcaggcttt
      721 gctggagcaa cgccaaagcg aaactcgtgc cgcacagtcg ctggttaatc aacgccaggc
      781 agaactggac tccgtagcaa aacgtcatac gcgttcccgc tcactggcac aacggggggc
      841 aatttctgcg caacagttgg atgacgaccg cgccgccgct gagagcgccc gagctgcgct
      901 ggaatcggca aaagctcagg tttctgcttc taaagccgct atagaggcgg cacgcaccaa
      961 tatcattcag gcgcaaactc gcgtcgaagc ggcacaagcc actgaacggc gcattgccgc
     1021 agatatcgat gacagcgaac tgaaagcccc gcgtgacgga cgcgtgcagt atcgggttgc
     1081 cgagccaggc gaagtgctgg cggcaggcgg tcgggtgctg aatatggtcg atctcagcga
     1141 cgtctatatg accttctttc tgccaaccga acaggcgggc acgctgaaac tgggcggtga
     1201 agcacgtttg attcttgatg ctgcaccaga tctgcgtatt cccgcgacca tcagttttgt
     1261 cgccagcgtc gcccagttca cgccaaaaac cgtcgaaacc agcgatgagc ggctgaaatt
     1321 gatgttccgt gtcaaagcgc gtatcccacc ggaattactc cagcagcatc tggaatatgt
     1381 caaaaccgga ttgccgggcg tagcgtgggt gcgggtgaat gaagaacttc cgtggcctga
     1441 cgacctggtg gtgaggttgc cgcaatgacg catctggaac tggttcccgt cccgcctgtc
     1501 gcgcaactgg cgggcgtgag ccagcattat ggaaaaaccg ttgcgctgaa caatatcact
     1561 ctcgatattc cggcccgctg catggttggg ctgattggtc cggatggcgt cgggaagtcg
     1621 agtttattgt cattgatttc cggtgctcgt gtcattgagc agggcaatgt gatggtgctg
     1681 ggcggcgata tgcgcgatcc gaagcatcgc cgtgacgtct gcccgcgcat cgcctggatg
     1741 ccgcaggggc tgggtaaaaa cctctaccac accttgtcgg tgtatgaaaa cgtcgatttt
     1801 ttcgcccgcc tgttcggtca cgacaaagcg gagcgggaag tgcgtattaa tgagctgctg
     1861 accagcaccg gattagcacc gtttcgcgat cgtccggcag ggaaactctc cggcgggatg
     1921 aagcaaaaac ttgggctgtg ctgtgcgtta atccacgacc cggaattgtt gattcttgat
     1981 gagccaacaa cgggggttga cccgctctct cgcgcccagt tctgggattt gatcgacagt
     2041 attcgccagc ggcagagcaa tatgagcgtg ctggttgcca ccgcctatat ggaagaggcg
     2101 gaacgcttcg actggctggt agcgatgaat gccggagaag tgctggcaac cggtagcgcc
     2161 gaagaactgc ggcagcaaac gcaaagtgcc acgctggaag aagcatttat aaatctgtta
     2221 ccgcaagcac aacggcaggc gcatcaggcg gtagtgatcc caccgtatca acctgaaaac
     2281 gcagagattg ccatcgaagc gcgcgatctg accatgcgtt ttggttcctt cgttgccgtt
     2341 gatcacgtca atttccgcat tccacgcggg gagatttttg gttttcttgg ttcgaacggc
     2401 tgtggtaaat ccaccaccat gaaaatgctc accggactgc tgcccgccag cgaaggtgag
     2461 gcgtggctgt tcgggcaacc ggttgatcca aaagatatcg atactcgtcg ccgggtgggc
     2521 tatatgtcgc aggcgttttc gctctataac gaactcaccg tgcggcaaaa cctggagtta
     2581 catgctcgtc tgtttcatat cccggaagcg gaaattcccg cgcgagtggc tgaaatgagc
     2641 gagcgtttta agctcaacga cgtcgaagat gtattgccgg agtcattgcc gctcggcatt
     2701 cgccagcggc tatcgctggc ggtggcggtg attcatcgcc cggagatgct aatcctcgat
     2761 gagccgactt ctggcgtcga tccggtggcg agggatatgt tctggcagtt gatggtggat
     2821 ctctcgcgtc aggacaaagt gactatcttc atctccaccc actttatgaa cgaagcggaa
     2881 cgttgtgacc gtatctcact gatgcacgct ggaaaagtgc tcgccagcgg tacaccgccg
     2941 gaactggttg agaaacgcgg agccgccagt ctggaagagg catttatcgc ttatttgcag
     3001 gaagcggcag ggcagagcaa cgaagccgaa gcgccgcctg tggtacatga cactacccac
     3061 gcgccacgtc agggattcag cctgcgccgt ctgtttagct acagccgccg cgaagcgctg
     3121 gaactgcgac gcgatccggt acgttcgacg ctggcgctga tgggaacggt gatcctgatg
     3181 ctgataatgg gttatggcat cagtatggat gtggaaaacc tgcgctttgc ggtgcttgac
     3241 cgcgatcaaa ccgtcagtag ccaggcgtgg acgctcaatc tctccggttc ccgttacttt
     3301 atcgaacagc cgccgctcac cagttatgac gagcttgatc gtcggatgcg tgcgggcgat
     3361 atcacggtgg caattgagat ccctcctaat ttcgggcgcg atatctcgcg tggtacgccc
     3421 gtgaaactcg gtgtctggat cgacggtgcg atgccgagcc gcgccgaaac ggtaaaaggc
     3481 tacgtgcagg ccatgcacca gagctggcta caggatgtgg cgagccgaca gtccacaccc
     3541 gccagccaaa gcgggctgat gaatattgag acgcgctatc gctataaccc ggacgtaaaa
     3601 agcctgccgg cgattgttcc ggcggtgatc ccgcttctgc tgatgatgat cccgtcaatg
     3661 ctaagcgccc ttagcgtggt gcgggaaaaa gagctagggt cgattatcaa cctttacgtg
     3721 acccccacca cccgtagcga atttttactt ggcaaacagc tgccatacat cgcgctgggg
     3781 atgctgaact ttttcctgct ctgcgccctg tcggtgtttg tgtttggcgt accgcataaa
     3841 ggcagtttcc tgactctcac cctggcggcg ctgctgtata tcatcatcgc caccggaatg
     3901 gggctgctga tctccacctt tatgaaaagc cagattgccg ccattttcgg tacggcaatt
     3961 atcacgttga tcccggcgac acagttttcc gggatgatcg atccggtagc ttcgctggaa
     4021 ggtccaggac gttggatcgg cgaggtttac ccgaccagtc attttctgac tatcgcccgc
     4081 ggaacgttct cgaaagcgct ggatctgact gatttgtggc aactttttat cccgttgctg
     4141 atagccatac cgctggtgat gggcttaagc atcctgctgc tgaaaaaaca ggagggatga
     4201 tgcgccattt acgtaatatt tttaatctgg gtatcaaaga gttgcgcagt ctgctcggtg
     4261 ataaagcgat gctgacgctg attgtcttct cgtttacggt gtcggtgtat tcgtcagcga
     4321 ccgttacgcc aggatcgttg aacctcgcgc cgatcgccat tgccgatatg gatcaatcgc
     4381 agttatcgaa ccggatcgtt aacagcttct atcgcccgtg gtttttgcca ccggagatga
     4441 tcaccgccga tgagatggat gccgggctgg acgccggacg ttataccttc gcgataaata
     4501 ttccgcctaa ttttcagcgt gatgtcctcg ccggacgcca gccggatatt caggtgaacg
     4561 ttgatgccac gcgcatgagc caggcattta ccggcaatgg gtatatccag aatattatca
     4621 acggtgaagt gaacagcttt gtcgcgcgct accgtgataa cagcgaaccg ttggtatcgc
     4681 tggaaacccg gatgcgcttt aacccgaacc ttgatcccgc gtggtttggc ggggtgatgg
     4741 cgatcatcaa caacattacc atgctggcga ttgtattgac cggatcggcg ctgatccgcg
     4801 agcgtgaaca cggcacggtg gaacacttac tggtgatgcc gataacgccg tttgagatca
     4861 tgatggcaaa ggtctggtcg atggggctgg tggtgctggt ggtgtcggga ttatcgctgg
     4921 tgctgatggt gaaaggcgtg ctgggcgtac cgatagaagg ctcgatcccg ctgtttatgc
     4981 tgggcgtggc gctcagtctg tttgccacca cgtcaatcgg catttttatg gggacgatag
     5041 cgcgttcaat gccgcaactg gggctgctgg tgattctggt gctgctgccg ctgcaaatgc
     5101 tttccggtgg ttccacgccg cgcgaaagta tgccgcagat ggtgcaggac attatgctga
     5161 ccatgccgac gacacacttt gttagcctcg cgcaggccat cctctaccgt ggtgccggat
     5221 tcgaaatcgt ctggccgcag ttcctgacgc tgatggcaat tggcggcgcg tttttcacca
     5281 ttgcgctgct gcgattcagg aagacgattg ggacaatggc gtaaggaagg gaaacttgac
     5341 ttcatattgt gatgtcatac tattgatgtc ataaagtgaa gtcataagga catgccatga
     5401 gaacccaggt acaggcttta cggaagaaac aaaaaaatac attggatcaa atatttaaat
     5461 ctcctgttcc tcaagggatt aagtggtctg atatagagtc actggttaaa gcattaggcg
     5521 gagaaattaa ggaaggaaga ggttcgcgtt gtaagttcat actaaatatg agcgttgcgt
     5581 gtttccatcg gcctcatccg tcgccagata ccgataaagg cgctgtagaa agcgtgcgtg
     5641 actggttact aagaaaacca tgatcaaact gaagacgcca aactctatgg agattgccgg
     5701 gcaacctgct gttatcactt acgtgccaga actgaatgca tttcgtggca aattcctggg
     5761 cttgtcaggt tattgtgatt ttgtctctga cagcattcag gggctgcaaa aagaagggga
     5821 gctttccctg cgagagtatc ttgaggattg taaagctgcg ggtattgagc cgtatgcgcg
     5881 tacagaaaag attaagacct ttacattacg ctatccagaa tcgctgagtg aacgccttaa
     5941 taatgcggct gcgcagcaac aagtctctgt aaatacttat attattgaaa cgctaaatga
     6001 gcgtttgaat catttataac ttatagcggc ccaacaattt ctgttgcgcc gctatatttt
     6061 tgtggctctt aagatgcctt tctaatcaat cgatgcccct cattcatcat caggattttg
     6121 ctatacacat cttcctcgac ggcatttttt gctgatgcca tcacccgtgc cagattcgcc
     6181 tcgttgtgat tattcacata cgctttgccg actgcggtga taaacgcttt gcgcagatcg
     6241 ctggcgatat tcactttagc gacgcgataa ttaacgaaac tgcgtaatat ctccggggcg
     6301 ataccggagc cgccgtgaat aaccagcggc accgggctga cttcggcaat gcgcttcagc
     6361 agggggatat cgatccgcgg tatatcgtcc aggccatgaa cgttaccaat agaaactgcc
     6421 agcatgtcgc aaccggtgcg ttcgacaaag gtcttcacct tttccggttc cgttttgcaa
     6481 tcggcttcgc tgacgtgatc atcttctttg ccgagaatcg cccccagctc cgcttcaacc
     6541 ggcacgccat aggatttaca gaaatcgaca gcttcctggg tgaaagcgat attctcttca
     6601 aaagagaatg cggcaccgtc aatcatgacc gaggtaaagc ccgcgcgcac cgcctgttta
     6661 acatcctcca gggttttccc gtgatccaga tgaagactga ccggaacatc cattttatcg
     6721 gcgtgacgtt tca
//
LOCUS       DAZGJT010000174         6595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZGJT010000174 DAZGJT010000000
VERSION     DAZGJT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.177"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(75..2366)
                     /gene="opgB"
                     /locus_tag="ACHKFK_004549"
     CDS             complement(75..2366)
                     /gene="opgB"
                     /locus_tag="ACHKFK_004549"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO9054729.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLAAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSRTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKAIWKGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(2620..3114)
                     /gene="yjjA"
                     /locus_tag="ACHKFK_004550"
     CDS             complement(2620..3114)
                     /gene="yjjA"
                     /locus_tag="ACHKFK_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO9054730.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNN
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNVAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            complement(3163..3900)
                     /gene="dnaC"
                     /locus_tag="ACHKFK_004551"
     CDS             complement(3163..3900)
                     /gene="dnaC"
                     /locus_tag="ACHKFK_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO9054731.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            complement(3903..4442)
                     /gene="dnaT"
                     /locus_tag="ACHKFK_004552"
     CDS             complement(3903..4442)
                     /gene="dnaT"
                     /locus_tag="ACHKFK_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO9054732.1"
                     /translation="MSSRVLTPDVVAIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTAEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            complement(4550..5023)
                     /gene="yjjB"
                     /locus_tag="ACHKFK_004553"
     CDS             complement(4550..5023)
                     /gene="yjjB"
                     /locus_tag="ACHKFK_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO9054733.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            complement(5014..5784)
                     /gene="yjjP"
                     /locus_tag="ACHKFK_004554"
     CDS             complement(5014..5784)
                     /gene="yjjP"
                     /locus_tag="ACHKFK_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO9054734.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFLASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            6404..>6595
                     /locus_tag="ACHKFK_004555"
     CDS             6404..>6595
                     /locus_tag="ACHKFK_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HFO9054735.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESE"
BASE COUNT         1628 a         1610 c         1656 g         1701 t
ORIGIN      
        1 tttctcaata atgttttgta ggccggagaa ggtgtgtagg tcgcacctta tgcgacctac
       61 acatcagtca ggaattaccc ttcacgttct accactttaa tttccaccat gccgatcccg
      121 agcttacgcg gcgagtgccc gaggatattc ccttcgttgg ttgagacagg ttccggtggc
      181 acaattacca gtgtgtcggc atcggttggg ttatcgaaat gcagtgtggt ggtggtcact
      241 tcattgccca gcacaagggt ttgttcttca ttgcctacgc gtaccggaat aggacggctg
      301 gcgttattgc cgtatgcttt ggcggtaatc accaggtcaa atttcttcgg cagcggatgc
      361 ttgtactcga tttttacttc atcgcccagc tgtgcgttgg accagcggcc ccacgactct
      421 ggacgagaaa tcccgctaaa ctgtttcacc tcttctggcg caccagcgac gttaaagatg
      481 aaactgtcgg ctttgtagcg aatatcgtta tcaacaatct ttagcgtatc gacattgcct
      541 ttgtaacgcg ccatgtcgat gaccgtatct ttaaatgccg tttttccctt ccatattgct
      601 ttgtcaacat gctgaacaat ttgctgaccg ccaagctgcc cttgcgagac acaccagtcg
      661 gtggagagtg ccagttccgg agcccagagt tgtgccatct tgtagcaacg gtcaacccag
      721 acgaaattgt cgcgtggagc gaaatctgcc agctggaaac gcagcggtgc tgagtattcg
      781 ctttccggca gcggttccac gcgtttgtct gaaacccgca acagcagcgg caaacggaaa
      841 tggctacctg agaaggcaat catgtttttc tgctggtcga tggtgaactc tttcatctct
      901 ttagggaatt tccacaggcg gatgatatcc ggcttccacg ccaatgtttt ctctttgata
      961 ttgaggaaga tttccgacat cgactgcccg gataaactgc tacggccaag tccgagatag
     1021 ttatcgccac cgagaatgtc cagcaccgtc gcaccgttat ccatcgtatt acgcttcact
     1081 gccagcgtct cttgctgcgg cttgtcgcca cggatgacaa aaaacaggtt attgcgatcc
     1141 tgcttattaa ggtatttcca cgccgtattg ttcatcgcta aatggtcaga agagacgacg
     1201 atgacggtat ctttaaacca cggtgacgct ttgattttgt tgataaacgt cgcaatattc
     1261 tcctggctgc aacttaccgc gctgaatgac tgattcggct taccgtcaaa atcatatttt
     1321 ttgcggttac aggtgcgcga gataaaacca tccgggtgat gggtatcgac tgtcagggta
     1381 aacagtgaga atcgctgacc tgagcgggaa agctcttcaa attttttcca cgcttcatcg
     1441 agaacggtat cgtcgtagaa tccccagtcg ttgcgatagt gcgggtcggc caccacgctt
     1501 ttcagctctt ctgagccgta taagtggtcg aagccgtgcg atttcaggaa cacatcttta
     1561 ccggcaaaac gcagattcgc gccctgcacg aaatagttct gataacccga gtttttcaga
     1621 atatcgccca gacagatgtt ctgcgggaag aagctggaga cagaggcgga ggcgttgcct
     1681 tcaaatgggg caaacagcgg tatgccgcac tgagaagcta ccatgcccgc aatcgtgtaa
     1741 tccgttcccg gcagctgctg cgtgtggctg aaatccaggc cttcattttt caacgcgccc
     1801 agttcaggcg tgagatccgg gaaagcctcg ttatcaaaat aggtccgctc gagactttcg
     1861 ccgtagatat aaaccaggtt gagtttcggg tcagggatag ttctcgacgg ctctttataa
     1921 taagccgcaa agtccgggtc gccgtcgcgt gactgggatt tcaccagttc cgttatctga
     1981 cgaaatgccg ggctggcatc cactgaaccc agcgccagta agagtgccag caggctgtaa
     2041 ccaaaatggt gcggatgatg gcgacgacgg cgcaggatcc agcccagcgc accgaacacc
     2101 gccgccagcc ccagcacaat gccgatacca ggcaggatgt atttgctgac accagcaccg
     2161 gtcaggctgt tggttaaggt atagagaacc gcgtcgttaa taccatcgcc agtaaaatag
     2221 tcgctggcaa acagggtgat atttaaaacg acaaatagcc ccagtaccgt taatgtggcc
     2281 gcaaaccacc aggtgttacg tcccgctttc catgcgtaaa tcagcacaga ggcgagaaaa
     2341 agggcgaaag agagtagttc tgacaacggg cgatcctcac taaaccgggc cgtttagcca
     2401 acgtttaagc tttttgagaa gcacaatgta atggcgtcgc catattgctg caattctagt
     2461 gtcaaaaaaa tgcgatgttg ttaggaatta gtttataaac agactttttt gatcgcggtc
     2521 gcttcccgtg taacaggggt aaaaccgtcc aggagcaggg aaaagggcag gagagagccg
     2581 cgtcagccgg gtaacagcta aaacgcggcg tttcgcggtt caggaaatga agttcagccc
     2641 ctgctttaac accagatcgc aggctttggt tttcaccttt tctgccagcg gagtcgttcc
     2701 aatattatcc agattgagtt gctgaccatc tttggttttc agcaaaccct gaatgccatc
     2761 cagatagttg gtgtcttctt tttgctcttc actgttcagg cccagttttt ccagtacctg
     2821 attcttgatg ttttcggcat cggttaccga agccagcttt tgcttcgcgc agtattgcag
     2881 aatgcctgcg acgttgttca tgttatctgc gcttaaggcc tggttcccgc tgctaagcaa
     2941 gttagttaat gacgcgagcg accaaccgcc ttcctgtgtc gtgttgtttt ggttgccaag
     3001 ttcgctggcg gcgctggaga gcgcatcttt ccaggacgca gcatgcaccc cggtggaaat
     3061 taatgcgctg gcggcaatgg cacagcacag aagatgttta atagttttca tcatcattac
     3121 tcaaggttga attgtgtcgc agtataccgc cagaaacggg gattaatact ctttacctgt
     3181 tacacggcta cggtagctat cccagttgaa gatcacccac aaactgttac ccaggcgcat
     3241 acggtccatc acgcgttcgc ccagcaactt ggtcatctct tccatattac tgttggtcag
     3301 catcccggtt gggcgtttgg aagaagagcg gcgatcgacg atctggttga tgatcacttt
     3361 ttcgtatttc gattcggtct gcacgccgat ctcatcgatc accagcagat caacgttact
     3421 cagatcgtta agcagttgtt cttcgctggt accgctattc ctgaaggtat ctttcatcgc
     3481 cgacataata tcggccacgg tgatgatcaa tacggattta ccgcgtagca gcagctcgtt
     3541 gcagattgcc gccgccagat ggtttttgcc ggttcctggc ttaccagaaa agatgaagct
     3601 ggcgatgttg ccgtcaaact cttcgacata ctggcgcgct ttgcttaacg cattcatttg
     3661 cccttcacac tcaacgcgat agttctcaaa ggagcagttc tgatgcagtg gacgaatacc
     3721 ggagcggtta aaggtgcgct gcattttcat cgcccgattt tcacgttcga gagcggcgga
     3781 gcggatcgcc ccttgttctt tctgccaggc cagtagttct tcacccgttt tgaaagcagg
     3841 tttgatatgg gcaggcatca ttttttgcag gcgttgcatc aagtcgccaa cgtttttcat
     3901 cgttaccctc tgaatcctgg tggaatttgg ctgtcaggtt cactgaccgt attcacatct
     3961 cgtttcggca gtccgccgtt gctggcacga ccgatttgca ggctgcgcgc cagtttttgt
     4021 tgccactgca catggtgaaa gactttgcct tccgcctgcc agtaggcaat gaatgacgcc
     4081 agttcttcgg cggtcaccgg ctctcttagt gccacgcccc atagcgccgc caggcggata
     4141 aagtcggcat cgggttgcca gtccgggtac atggcaaatt tccccattgg tacagcaacg
     4201 ggagcggctt gcggttcctg atagagttga tcgtccagag cgacatcgct tcccggacgc
     4261 gccagctttt cttccagcgc cagcagttca gccaggcgtg caggcgtgac ggcataaaac
     4321 gccggggcat tgttagcaaa tacggcaacc acaccgcctt cagcttttgc cagaacggtt
     4381 tggtgatcgt gtaccagggc gtcaatagca acgacgtccg gggtcaaaac tctggaagac
     4441 atactgtttc tcaaatatgg aacgatgaac gagaagggag ataactttta tagtaacaca
     4501 cggaggagag gaggctgtct ctcatccgcg tgctttaagt gaggcaattt tatacgcgag
     4561 ggcgtttgcg gtacaaccat aatccaggaa tggaaagacc gatggataac gcaccaacaa
     4621 tcgatgaagc tgtaagaaag ttggttaaca gggtaatcat caacggttcg ctgtaaccta
     4681 gctggctgat ttttaccgcc gaaatcattg cggtataagc tgatatgcca gggaacatag
     4741 ggataacagc cgccacggta aacactttcg gatgcgccag ataccagcgc gaccattgaa
     4801 taccaatggt gccgaccagc atagaggcca taaaggttga ccattcaata ttcagcccgc
     4861 tggtcattaa gatcattcgg gaaccatgac ctatcgcacc aagcagcgca caccagcgta
     4921 acgctcgaac gggaacgttg aacaccatcg caaagccgac cgcaggaatg gcggcgagga
     4981 tcatatcctg cgccaacgct aacagaaatt cgatcacacc catccgcgta gcccccaaat
     5041 cgtcagtgcc attactacgc cgacacaggt agccagtgtc agcagactgg cgattgccca
     5101 gcgtgccagt ccggtattga tgtggccttt aaacatatcg gcgacggcat ttatcaacgg
     5161 aaagcccggc accagcagca gaacgctggc ggccatcgca atggtggggg tattgctgaa
     5221 agtgggaagt tgcaaaagca atccggaaat ggtggtggcg gcgaaagcgg tgaggcaaaa
     5281 gttgatctgt ggatgaagat gacgttgtgc cagcagctgg cggatataca tcgcggccgt
     5341 actggccagg aaggtgatga cggcaccatc ccagccacct ttattgagtt tacagaaaca
     5401 ggcgcaggaa aggccaacca ttaaggctac cagccatctt gggtaacgta atggctgaat
     5461 ttggctaaat cgtttctcta cgcctttgta atccagcaga tgatgctccg ccagaattac
     5521 aatgtgctgg acttcagtca ccacatgcat attaatgccg cgatcgtgat ttttacgtgt
     5581 cgatgtcagg cattgcccat ctttaatggt tgtcagcact atggcgttcg aagagataga
     5641 actttcgacg ctgtccattc ccaacgcccg acccagtcgt gaggaaagct catcaaccaa
     5701 cgcgctttcc gcaccatgtt gcaaaagaaa taatccacac tggatacaaa gccgtgttac
     5761 ggctcgctgt tgctcagttt gcataactcg ccctttgtca tagacgtgct gcgtattgtt
     5821 cagataatga gaatccattt gtatcatgaa gcacggttgt tttgcggtag gttaagatca
     5881 aaattgcgct gattaatcct ggttatcggg agatgctcgg tgataaaaag cgaattatct
     5941 ctcgccgtaa acaaactatc gcatgaatat tatcactata atgaatttgt tatttggcct
     6001 ttacgagtca ggataataga tagctgggcg cgttttttaa tgttaacaag ttaaaaccat
     6061 cagattttat tgaataaata ttgattgtgt gattcccgca ctctgcggga agagatatca
     6121 tttaattata aattaaatga atgagatttc tgtttttcat taagtatttc taacaactta
     6181 atgtagatat tttaaatgtc tccaggctat ttcaccaggt tatcatttga tgctaacctt
     6241 ctgacacatg cagtggagtt gttgtgcagc aggagtatgc tgatatgaaa gtgaatgcta
     6301 aggataattt attcgcttaa tctattaatt tgctgggaat atttaaggag tgaatacctt
     6361 gaataaacca tcatgatttt tagacagtgg atgtggagga aatatgttgc caggatgctg
     6421 caaaaatgga attgttatca gtaaaatacc tgttatgcaa gcagggttaa aagaggtcat
     6481 gaggactcac ttccctgaat atgaaataat atccagcgcc tctgcggagg accttacctt
     6541 attacaatta cgtcgttccg gattagtcat tgctgattta gccggtgaaa gtgaa
//
LOCUS       DAZGJT010000175         6488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZGJT010000175 DAZGJT010000000
VERSION     DAZGJT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.179"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..858
                     /locus_tag="ACHKFK_004556"
     CDS             <1..858
                     /locus_tag="ACHKFK_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO9054736.1"
                     /translation="IKNNVEHLLKSESTALRESICAVIAGKDGYQADTLSLLPGVRII
                     SRFGTGIDNIDLRAAQQSGIVVNNAVGINSNAVAEFIIGLIFASMRNIPGSYHAMQNG
                     YWGESHGCELQGKRIGLVGYGNIGKTLAKRLSGFDVELLAFDKQPDYQVADKAGVQFV
                     SIEDIFMQSHVIIVLLPFSSGLENFISHKYLSMMRNGALIINVARGKLLDEGALLQVI
                     EERNVFAALDVFSSEPLAQFSPLLHAKNIITTPHIAAATVESYQQTGIHVAQSIIDYF
                     AGREIKNVL"
     gene            complement(1158..2534)
                     /locus_tag="ACHKFK_004557"
     CDS             complement(1158..2534)
                     /locus_tag="ACHKFK_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO9054737.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     QVEKLCLALFRQNGVELQTLVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDL
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMNQPKKSEESPQPVFATRSVQQNDK
                     DASPPVPSPEISRQRTWPIFMAGMVVMAGLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PLSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSAEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            complement(2534..2962)
                     /gene="tssE"
                     /locus_tag="ACHKFK_004558"
     CDS             complement(2534..2962)
                     /gene="tssE"
                     /locus_tag="ACHKFK_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO9054738.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     HLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            complement(2966..3502)
                     /gene="tssJ"
                     /locus_tag="ACHKFK_004559"
     CDS             complement(2966..3502)
                     /gene="tssJ"
                     /locus_tag="ACHKFK_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO9054739.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKN
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYYPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            complement(3483..4562)
                     /gene="tssG"
                     /locus_tag="ACHKFK_004560"
     CDS             complement(3483..4562)
                     /gene="tssG"
                     /locus_tag="ACHKFK_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO9054740.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQLLEQSQQNAPLGST
                     DSPATDAVRFRPHPGMGFPVSELKNVERDVGNPDAPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAVDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSPISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPAFSIRNRESMTHVSYSF"
     gene            complement(4526..6286)
                     /gene="tssF"
                     /locus_tag="ACHKFK_004561"
     CDS             complement(4526..6286)
                     /gene="tssF"
                     /locus_tag="ACHKFK_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO9054741.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCVYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFA
                     CGPLVGDWSQIDLSRLPLYLNADSPVACALHRALTLGTQQFWLRLPGQDRRMLGAHFS
                     PMGFEDDDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLEQVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPLEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDTDRMLEDETLSLSLTGTNGQLPRKALQSTLLDTPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
BASE COUNT         1533 a         1729 c         1718 g         1508 t
ORIGIN      
        1 attaagaata atgtggagca tctgcttaaa tcagaatcca ctgcattacg agaatctatt
       61 tgcgcggtca tcgccggaaa agatggttat caggcggaca cgctatcatt gctgcctggt
      121 gtgagaataa tatcgagatt cgggacgggg atagacaata ttgatcttcg tgcggcacag
      181 caatccggta ttgttgtcaa taatgcggtt gggattaatt caaatgcggt tgcggaattt
      241 atcattggac ttatcttcgc gagcatgaga aatatccctg gcagctatca tgcgatgcaa
      301 aatggctact ggggtgagtc gcatggctgt gaattacaag ggaaacggat tggcctggtt
      361 ggctacggta atataggtaa aactctggcg aaaaggttat ccggttttga tgttgagctt
      421 ttagcttttg acaaacaacc cgactatcag gttgctgaca aagctggagt tcagtttgta
      481 tcaattgaag atatctttat gcagtcgcat gtcatcattg ttcttttgcc tttttcttct
      541 gggctggaga acttcattag tcataaatat ttgtcgatga tgcgcaatgg tgctctgatc
      601 atcaatgtcg caaggggaaa gttgctggat gagggcgctt tacttcaggt gattgaagaa
      661 cgaaatgtgt ttgcggcgct ggatgtgttt agctctgaac cgttggcgca atttagccca
      721 ctgctacatg ccaaaaatat cattacaacg ccgcatatcg cggccgccac cgttgagtca
      781 taccagcaaa ccgggataca cgttgcccag tcgataattg attacttcgc aggaagggag
      841 ataaaaaacg tgttgtgagt tcagcgaaca aagattttaa atacaaaaaa accgcaattt
      901 gtagaacaca ttgcggtttg tcagcattct aataaattgg ttgcgggggc cacagtcaag
      961 aggttaatcc aatcttttga aacttaaggt attcactgtt cccgaaccca agctatctac
     1021 cgaaatatac actcattatc tgatgtgaat caacgagtag atcgaatggc ctgttcctct
     1081 ctttttttgt acacagagac tcctttaaaa aatggcaagg tcacatatcc cttattggta
     1141 cataaatccc ccgatgttta ctgactttgt tccaccttcg tatccagtaa aatgacatag
     1201 cgattcaaca actgatttaa ccgggtatca atttgcgtca acagcgttgg cgaaacctct
     1261 ccggtctgtt tttgctcttc cagttgccga agcagttctt ccagcggcgg ctcctttaac
     1321 gactgccgga tgccgtacac tgccgttttt agttccgaac cggtcagata tctcccctta
     1381 cgttcatcca gtgcatccag ctgcgccgac aggcgctgta gctggctctg cgcctgagca
     1441 tagccattca gtgcctcagc agacagggcg ctcagctccc gctgtttgcg ccactgcgcg
     1501 ttcagcgccc tgacctgagg attatccggc cacagggcat caagctggcg tagctggcga
     1561 tatccctgct ccagtggcca gcgggcaggc aatttgttta actgctccag ttgcatctga
     1621 cttgccgcaa ttgttctgtc ctgagccagc agcgccttat cctttacatc cagctttgcc
     1681 agttcgccgc tctccagcga cagcggcaac ggcatgacag acagttgtat tcgctggatt
     1741 agcgcgtccg gctgattaag ctgcgaccag ccccataatc ctgttccgcc gagaccggcc
     1801 atcacaacca ttccggccat aaatatcggc catgtccgct gccgggagat ttcaggggat
     1861 ggaacgggtg gactggcatc cttatcgttt tgctgaacgg atctggttgc aaaaaccggc
     1921 tgtggacttt cctctgactt tttcggttga ttcatttccc ggcggggtaa ttcaggggga
     1981 gtgatagtgg tttccgctcc ctgaggcgcc agccgttcca gacgcaatgc ggtattgcga
     2041 aactgcccgg ccagtagatc cagtttcgtc atatgccaca gttcacactg ttgcagcgtg
     2101 gtttcgatgg cggaaagatg ctgtacacaa cggtagatct gcggcagatc ctgatactga
     2161 atatccagcg ttctcagtgc ctgctgcatt ttttctgtga cccagctgag cagtgatatt
     2221 ctggaatgta cctgtaccgg ccagaagtca gcccagcgct ggagcagtat atccagtgaa
     2281 ccgagtccgt ctgccatccc tgccagcccc tgccgtctgg ttatcgccag tacgtaacag
     2341 accagcgtct gtagttccac gccattttgc cggaacagcg cgaggcagag tttttccacc
     2401 tgacgccagt ccacatccgg tcgggccggg tgagtcagtt tactcatttc agcctgcagt
     2461 tcaccggatt ccttataagt acagggatcg ccgcccatga cgaactggct gataaaattc
     2521 gcgttacttg ccatcagtac gcccgctcct gttgtttgag atgacgcaga acaattcgtc
     2581 cttccggaga aaatacgcca tcaaagcgta tccagccggt atcgggaagc tgcgcgtgga
     2641 tcaggtaatt aagatgcccc ggacggggct gggggagtaa ctccacctgt atctgactca
     2701 ggcgtggttc ataacgcagc agcgtttctt caatctgacg catcaggcca tgaatacccg
     2761 cagccatccc ctgatggatc agactgagat ccggcagacc gtaatccggc aaatgcgcca
     2821 gcgtgcctgc ccgacagttg ataatgcgtt gcatattgtc catcacggaa aggataagct
     2881 gatcccgctc gctgacctgt tccagcggta actcgccgct gaaactgaac gtcagcattt
     2941 catataagga aggtgtcgac atcggttatt tatccttgag aggcagcagt cgcaggtcgc
     3001 ttctcatcag ttcaatcgag cgcggcttgt cggcctccag ttcgtccctt ttgatcacca
     3061 gacgccagct gtcgcttttt tcatcagggt aataaaactg cccaataatg gcgacaaact
     3121 gcgtctcttt atccagcggc acattcaggg atgccgtgcc ttccggtttt acccagacgg
     3181 tgtgttttgc cagtacgttc ttctccagcg tcttctcttc ctgcataaac agggtgtcat
     3241 aatccgcctt gtcaaaatct tcgcgggttt tcagttgcca gacatgcacc atcgtggaaa
     3301 gcggcgtatc ttccgcatcc gtattcaggg cacttcggga gaagaaatcg agatgcagta
     3361 ttttgatttg cttgtagaaa acggctgagg ccacgctttt cgattcatcc accactttct
     3421 gaatcagccc gcagccgctc agaacaaccg tcagcatcgc catacagagc agcgccggac
     3481 gtttaaaacg aataggaaac atgagtcata ctctccctgt tacgaatgga aaaagcgggt
     3541 attaatccct gataacgccc cagactgacc gtcactgttc cggtcgcggg cgcggcaaag
     3601 gaggcgcgaa ggatgccggt acggctaatc tgtacacgct gttttcccag ccgggcagca
     3661 ggcaacaggc tgacgggcag cgtcagttgc agtcgggcct gacagcgcca gcccagataa
     3721 acgcgcagca gcaccagcaa atcgctgtgc agttgcccgc cgggcagcca gccacgtgct
     3781 tctgcggcat cttcggtata cagtttcagc agcaactggc tgttgacgtc ggtgccggta
     3841 cgccccagca gcgttcgcgt tttcaggctg atgggtgaat tttttgacag acccgcaggg
     3901 gccgggagaa ttacaggctg cggatcatga ggcaccaccg tcgctttcgt cagcggggca
     3961 agcagtccca ccagtgccgt aacgccttcc gccgtgcgcg tcggcaggcg catcacgctg
     4021 agcagggcca gaaaacggga taccggcgtg gcgatatggt tctcgctgcc gggaataccc
     4081 agcccgatga gtcccagcag gcaacgggaa atgtcatcca ctgcacctgc ttcaaacgac
     4141 gccgggtagt tatatttgcg ccagatgcgg taatactggg tgataaagcg atggttaaaa
     4201 atatcgagaa acgccatcac cgcgtcatgt ccgtcatgac gctgggtaat gtagtccaga
     4261 taggcggttg gcagcggcga atccacgccg tacaggccga gaaaagtggt gcgcaccgtg
     4321 gggggcgcgt cgggattgcc cacgtcacgt tcaacgtttt ttaactcgct gaccggaaag
     4381 cccataccgg gatgaggacg aaagcgcacc gcatcggtgg cggggctgtc ggtactgccc
     4441 agcggcgcgt tctgctggct ctgttccagc aactggcaaa aacggtaaaa attgatccgc
     4501 gcaatatccg gggccagccg gtcggtcagc ccggtacgcg ctggctgtga tgctctgtcc
     4561 attgcaggca ctttcctgtg ggttgcagaa ttaaggtcag ttgagtgaat aagtggatat
     4621 cggcatacag tgcgaagaag cggtgcagca gttcaccaaa tagcgtgata tcaccttctc
     4681 cggcaaagcc gttactgtcc agcgtcacct gaatatccac gccacgcagc aaaaagcctc
     4741 tttcaaaacg ctgtatcagg ctgtgctgaa cgtcgacaat cgcctccaga cgacggcggt
     4801 tcatctcgct ttcggtccag tcgtacaatg ccagcgttcc gcgcagaatt tcggcgttat
     4861 ccatcatcga taaaaagtta gaaccaaggt gactgagcac ccgccagtgg aagcggtcac
     4921 gcgccggggg atagcagggc tgcgtcggag cgcacaggtt gcgcacccgc aggacgttct
     4981 gtgaggcatg aactggcgta tcgagcagcg tgctctgtaa agccttacgg ggaagctgcc
     5041 cgttagtacc ggtcaggctt aaagagagcg tttcatcctc cagcatccgg tcagtatcaa
     5101 atgcatcgcc gcccagaatc agccaggtgt catgcaggcc ggaagggccg cgctttaccc
     5161 gcgtgtggta atagcgttcc ggcgcatcgt ggcgcagcat cccgccccgg tgacgaaagc
     5221 tggagaaggg gacatacgcc tgactgccgg tatggcgcga agaaataatg ttgtcgacgg
     5281 aataaatctc ggtatgcccg tcctgaatgc gcatcgggcg cagcagaaat tcgttttcca
     5341 gtggcgaaag gtgtaacgga tcggcttcga gcgggaacag gttgatgacc ggcacgcagt
     5401 gcagacggat attatcgctg tcaaacggca ggtcgtgagg ccagttctcg ttcagcacca
     5461 cgtcaatctc aaagccggtt atcccctccg gccatgcaac ctgctccagc ccgttcagcg
     5521 ccacgaacat aaacttctcg cggaaggtga aatactccag cagcaactgg taaccgctga
     5581 aggctgactc accttttggc cagagcctgt catcatcctc aaagcccatc ggggaaaagt
     5641 gtgcgccaag catccggcga tcctgccccg gcagacgcag ccagaactgc tgcgtgccaa
     5701 gcgttaaagc ccggtgcaac gcacaggcta ccgggctgtc ggcattgaga tagagcggca
     5761 ggcgggataa atcgatctgc gaccagtcac caaccagcgg gccgcacgca aagcgcagac
     5821 ggataaccga gcgtccgtcc ggttcatatt gcaatgacac gtccggcaac gccagcggat
     5881 gcagcgtgat atcacgcgtg gcactgtaga cgcagcgggt tctgcgctcg ccaacagggc
     5941 gcgacaatac ctgaaaatcg ctcagcgttt cgctctgctt catctgccgg tgatctgtcg
     6001 acagttcaac cacggaaagt gacgggatgg ttcgcaggta gtggggccac agcaggctga
     6061 ccagcccttc agtcagttcc ggcagatcgt cgtccagctt ttcgcgcagg cggcccatta
     6121 aaaaagcgaa gccctcgaac aggcgctcca catagggatc gcgtgcgccg ggtttatcaa
     6181 gattaagcat cgcagcacgg tcggggtaag cctgcgcaaa ttctttaccc gcttcacgca
     6241 ggtagcgcat ttcggcttca tagtagcgtt gggtcaggtc gtccattcag agagttattt
     6301 cccatttaac taaaaagtta gataacagta aaaagaaaat atattttaca gttcataaaa
     6361 caccagtaaa aaacaacaat aacccataaa gatgagcgca attaaaaaac cattacaatc
     6421 atctgttaaa aataaacaat gactactttg cttatagtat tgccggtcag ttacttcatc
     6481 ttcctggg
//
LOCUS       DAZGJT010000176         6453 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZGJT010000176 DAZGJT010000000
VERSION     DAZGJT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.180"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..472)
                     /locus_tag="ACHKFK_004562"
     CDS             complement(<1..472)
                     /locus_tag="ACHKFK_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HFO9054742.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIAD"
     gene            complement(472..1275)
                     /locus_tag="ACHKFK_004563"
                     /note="aph(3'')-Ib"
     CDS             complement(472..1275)
                     /locus_tag="ACHKFK_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9054743.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            complement(1336..2151)
                     /gene="sul2"
                     /locus_tag="ACHKFK_004564"
     CDS             complement(1336..2151)
                     /gene="sul2"
                     /locus_tag="ACHKFK_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO9054744.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            complement(3015..5318)
                     /gene="trbC"
                     /locus_tag="ACHKFK_004565"
     CDS             complement(3015..5318)
                     /gene="trbC"
                     /locus_tag="ACHKFK_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO9054745.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            complement(5299..6423)
                     /locus_tag="ACHKFK_004566"
     CDS             complement(5299..6423)
                     /locus_tag="ACHKFK_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO9054746.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
BASE COUNT         1447 a         1674 c         1796 g         1536 t
ORIGIN      
        1 gatcggctat aatcgccgcg tggacgtagt cagtttgaca acctgcgttt tgatcatcgc
       61 gcgcccgctg aaacaaagct gcaaagcgat cccggatcgg gagaagggca gaaggcaggg
      121 gttcctcaga tgcggcatac agcttcgcca ttagttccgc tgcaatttcg gtcgcctggt
      181 agtcgccgtg ctcggcaacg atgtgagaga gcattcgctc cccggcatat tcgagcaaca
      241 tcagattgtt ctcacgaccg agcaaccgga ctgctcccct cccattgcgc cataccagat
      301 agtcggcccc gcgcagttca tcagcaatgt cttctatagg tttcaatccc ttgacgattg
      361 caggagtccc gtctggcaat gaaactttcc aaacgaggct ggaaaaggtg tccgcaatga
      421 gaacaggttg cgaaacgtgc caatgagcag gaaaaacagg cggcatgaac atcaacccca
      481 agtcagaggg tccaatcgca gatagaaggc aaggcgttcg cggtcggggg cttcgatccc
      541 caatacattg aataggacag cgaaggcgcg ctctgcttca tctggcgctg cccagttctc
      601 ttcggcgtta gcaatcatga gtgccaaatc ggcatagcga tctgctgttc cgagccgccc
      661 aaggtcgatc agacccgtgc attgaagagt tttagggtcc accatgaagt tcggcatgca
      721 gggatcacca tggcaaacaa ccatatcggt gcgctcttgg tcgagccgca ccggtagctc
      781 tcgttcgaca cgagccaaaa gatcgagctg cggcgtactc ttgtcctcgt ccggtaagaa
      841 gtcgggattg acggcattgc gggacaccac atcaacggcg cgtccgaaca ttcgcgacag
      901 cctgcgctca aacggacatt gatcaaccga taggctgtga acagcgccaa gttgctgccc
      961 cattgacggc cacgctttga gcaaatccgc tccagacaga tcagccgccg gtactcccgg
     1021 aattgccgtt atcaccaagc atgcaccctc ctgttcctcc tgccagttga tgacctcggg
     1081 gcaagccaca cctcgacctt tgagccaaat gaggcggtca cgctctccag cgagctcacc
     1141 gcggcgggaa gcaggtgcga ttttcgcgaa ggcatgcccg tcaccacgtc gaaaaacaaa
     1201 atcaccagat tctccgcctc tgacaggcaa ccagtcagaa tgcgattcac caaaaaaaat
     1261 attagttcga ttcaatggag gttccttcag ttttctgatg aagcgcgaat atagagaaat
     1321 atcccgaatg tgcagttaac gaattcttgc ggtttctttc agcgccgcca ataccgccag
     1381 cccgtcgcgc aaggggcgcg gctcgtgtgt gcggatgaag tcagctccac ctgcggcggc
     1441 ggcaagctct gcagcgagtg tcgcggcccc gacatccccc ggaccacggc ctgtgagcgc
     1501 gcgcagaaag gatttgcgcg aaacagacag aagcaccggc aaatcgaagc gcagccgcaa
     1561 ttcatcgaac cgcgccagca ccgagagcga ggtttcggga gcagccccca gaaaaaaccc
     1621 catgccggga tcaaggacaa ggcggttgcg tttgataccg gcacccgtca gcgccgcgat
     1681 gcgcgcgtca aagaacgccg caatgtgatc catgatgtcg ccagcgggtg cctcgcgccg
     1741 atctgcctgc ccgtcttgca ccgaatgcat aacgacgagt ttggcagatg atttcgccaa
     1801 ttgcggatag aacgcagcgt ctggaaaacc gcgaatatca ttgagatagg ccacaccacg
     1861 cgacaaggca taggcttgcg tcgcgggttg ataactgtcg agcgagacgg gaatgccatc
     1921 tgccttgagc gcgtccagca ccggcgcgat acgcgcgatt tctgtgtcgg acgaaacagg
     1981 cgcggcgtcg ggattgctgg atgccggacc gaggtcgatc acatctgccc cctcggccat
     2041 cagcttacgc gcctgcgcaa tggctgcgtc tggcgccaga taccggcctc catcggagaa
     2101 actgtccgag gttatgttga cgatgccgaa aatgatgagc gatttattca tgggggcttc
     2161 tataataata ataatcgagc atgagtctca tacggatgct cgggtcgaaa gggaatcccc
     2221 aggcgagtaa cctgtttgcg gtgatccatt agctgcagga gcagaatagc atacatctgg
     2281 aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag cgctcagtag gcaatttttc
     2341 aaaatattgt taagcctttt ctgagcatgg tatttttcat ggtattacca attagcagga
     2401 aaataagcca ttgaatataa aagataaaaa tgtcttgttt acaatagagt gggatctggg
     2461 aattcatacc aatatccata gaagtgaatc tattacgaac gtggggaacc ctggcaccat
     2521 tttttgtgct gcccatattt gttttcatgt ataattcata atcaaagcgt tcatcctgat
     2581 ttagcttaac tgattaaaat ccgtagatta tgattttatc agttttgtga ctttatttat
     2641 tatggggagc ttacccagta tttcgaaatt atcgggagaa cacttgtacc atctgtttga
     2701 gcctgcaagt atttctctgt cccatcgtct gaccctctcc ccgtttctga gtatgcatat
     2761 catatagttt tcgggcacca tcagtgtccg ggctgccaga agactatttt taaacgtcag
     2821 ggaagtgata ttgatatctt ctccggaatt tttacgataa ataacagacc atgagtttgt
     2881 ttccgggcta ctaatacaca tttattgtac ctctggtgat ggattgaaat cctgaatcct
     2941 ccgcttacta tggtgagcgg aggagattaa taatcgcgta aaggttgtta ttaaaatcag
     3001 ccatacaggt gttatcagaa tcttttttta cgactctctt gttttccacc tgatggtgtc
     3061 tgagcgacat tttttttatg gggcggggat gcagttagat ccggtacagg taacagcatc
     3121 tccaggggga gcagagtatt cagaatcgcc ttcactgaag gtatatcttc aggaccggag
     3181 tgttcctctt ccggacgatt cagtgtgata taacataccc ggcgttcttc cctggttgtg
     3241 ttattgatag tgtgcacagc ctttttccac agttcgtttg cccgggattc gatatctgta
     3301 tcatattgcg gaagcagatc cagtgatgtc tgcgctgttg ccaactggcg ctggaatgta
     3361 tcaatgatac ggtaaggctc tgtacggttt ttacgacgtt tacgggatgg ttttgctgtc
     3421 agtacaccga tgatgctgct gacatgctcc ggtgtgggga gcctgcgctg cactgtcctg
     3481 gggacgagac ggcggagacg ctccagccgg ggggcttcaa cggaaatata gcggttgatg
     3541 accaccggat cactgtcgca acttttctcg ctgtccggaa tatacacaga ggctgacggc
     3601 accacatccc cctggaaaac ggtaaagaac tcaccagcat tcagtctgat caaatcctgc
     3661 acgtcgattt tgctttccat ctggatggtg atctggttac cgtccaccca ggactcaccc
     3721 atcacaccat cgtggcgctg caggctgccc attctggccc gtgcctcttc accggcggca
     3781 tttttcaccg ttcttgccgt tttgtcgtca ctgacaatgc gtccggcgaa tttggtgccg
     3841 gcgttctgca tcagtgtggc ggtgttggtg gcggaggtct gcccttcaat tcgctcctga
     3901 tcctgcgccg tcattatcag ggaaaactcc agtgaacgta cctgggtggc ctccacagca
     3961 atgcggtctg tgtagtaggc accgacttcg tcaaggatac agatataggg gaagctgcct
     4021 ttgtattttt tgacttccag ggtctgtgca tctgtgcctt ccagccggta gcccaggtca
     4081 cgggcgagga gcatgctctg ctgggtgacg aacatacgac cgagtgccga cgtggtatga
     4141 gccgatgtgt ccagtgcagg gatcatcaca atcagaatgc ggtcgctgtg aatactgtcg
     4201 cgaatatcga tgtcgccggc atctgccgca aagacgtccc cgaaggtttc ggcaaaggtt
     4261 gtgaacgttt ccgagaactg accggagaga taagcatgct gtttacgggg ctcctcagtc
     4321 caggctgacg gcgttctcac cagtgacata tcgaatcccg ggacatcctg caggtagttt
     4381 cgcagtggtg cgatagcttc ttccggccac tggtcatcaa ggccacggca atagagtttt
     4441 gccatccctt ccagcgtcat atgttcccgt aacatctgca gggacatggt tttaccttcc
     4501 cggacgcacc agaattttgt gccaaagacc agggccttgt tcatggctat cgcccgtgac
     4561 tgccattcac cgccctgaac gttctggggc agcatggact gcatggtttc ggcggtgaag
     4621 gcttcggtgc tgaatgcgaa cgggttccag gtgtttgact gtgggcggga tttttcgccg
     4681 ctctgaatga tttcactgcg ggattttccg ccgttcatga agttgatgac ttcgatatca
     4741 tcctccctgc caaaacgtcg ggagagatac cagattgtcc tcgtggtatc gttctgtgcc
     4801 tttccgtcaa caaaggtaaa gcctcttccc cagcagagcg ggttgagcag ccaggcaaag
     4861 gtggtttctg ttttaccgcc accggtggtg gcaaaaaaca tgacatgccg ggtgaggtca
     4921 tccatgctca gccagagctc acgaccgata tcgttgatac ggcggtaacc gacataaaaa
     4981 atcccccggg cggggaatgt ttcatccgct tcatactgaa acagggtggg ccagaagctg
     5041 aagaggcttc tcctgacctt tctgtcctgg gaggggtcat caaggctgag tgtcatgggc
     5101 atacgcaggg ggcatcgcca gcgttgcagc atgaatatca cggaggtgac cagggtgaac
     5161 agcaggcaga caggaagggc cagtggccat atcagcgccg taatgatact gccggccaga
     5221 cacagaccag tgatgttcag gttatgcagt gcattccaca acgggtttcc ccaggccgtg
     5281 cggtgtatga ggtcaggatt aacgtggtgc tggctcattg tgatactcct cgctatctgt
     5341 tgctttcagg aagaggcgaa cggaagtggc gtctttcagg atgccggacg gcaggtgcca
     5401 gccggtatct gataacaccc agggggttcc gccaaaaccg aactggcgga aagcctggtt
     5461 attgacatcg atggcagcac gtgcggcggt cagacactgt ccatcctgtg cggccggtgc
     5521 atcgccggtg ggggccatac cactgtcagc ggaatacagc tgatgccatc cggtcgcccg
     5581 tttttgtgag tccggttcac acagcagcgg agcagtccgc tttgtactgg actccccgcc
     5641 aatcacggag accggataaa tcaccacgct gaattcccgg gccagttgct taagcgcggg
     5701 ttccagcaga cggcagttga tgcatgcggg gtcaaggaag gcgtacagcg tgcgttcatg
     5761 accagtggac aggttaatgg tgaacatccc ccggtctgcg ttgcgtttaa gtactgtggc
     5821 cagattacgg cgggcctcag cggcctgaac gtgttgttct tccgtcactg ccgtcggggc
     5881 gggggcagaa gaaggttcct gctgtgtggg agccgggcgg gccgggatgc tgttaacatg
     5941 atgttccgta tgtgggcgtg gtgtctctgt gtcggttact ggttcagctc tccattcctg
     6001 agatgtaggg taagccagcg tggaaggggg ctctgccttc atccagtggt aacctgcaac
     6061 tgtcgccggc agagcaattc cggcgataaa gccacacatc agtgtccgcc agaagccatt
     6121 tttccgggag ggatgttttc cttttccggc atattcggca tccaggcagg catcaatcgc
     6181 tgagagcatt ctgccgatgt gatgtccggg acgttctgca tcaacggtat caacaagcac
     6241 cgtttcggtt atttcgcgaa aaggggaact gaaggacagg tgcatcagcc tgttattacg
     6301 gttaatcagt gcgcccagtt ctgtgactgg caggatggca atcacctccc gattaccgtc
     6361 agggtacagg gcgatcaggg acaggtgttt attattcagt gttgcagtgt agtggatggt
     6421 cattgttctt tttccttgtc ggcgggatgc tgt
//
LOCUS       DAZGJT010000177         6448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZGJT010000177 DAZGJT010000000
VERSION     DAZGJT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.181"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..468
                     /gene="ydfJ"
                     /locus_tag="ACHKFK_004567"
     CDS             <1..468
                     /gene="ydfJ"
                     /locus_tag="ACHKFK_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HFO9054747.1"
                     /translation="SDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMVALIV
                     IHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCTMTES
                     WYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPHVVRAAQPSRSL"
     gene            557..2017
                     /gene="ydfI"
                     /locus_tag="ACHKFK_004568"
     CDS             557..2017
                     /gene="ydfI"
                     /locus_tag="ACHKFK_004568"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFO9054748.1"
                     /translation="MGNHLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQI
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVVADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMCDVVTSYAQAIDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYALMLQEQVP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGTARVQSLLAIKAIF
                     GDDLPGNSLFTTKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2053..2256)
                     /gene="ydfZ"
                     /locus_tag="ACHKFK_004569"
     CDS             complement(2053..2256)
                     /gene="ydfZ"
                     /locus_tag="ACHKFK_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO9054749.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2433..3119)
                     /gene="rspR"
                     /locus_tag="ACHKFK_004570"
                     /gene_synonym="ydfH"
     CDS             complement(2433..3119)
                     /gene="rspR"
                     /locus_tag="ACHKFK_004570"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO9054750.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3208..3954)
                     /gene="ydfG"
                     /locus_tag="ACHKFK_004571"
     CDS             complement(3208..3954)
                     /gene="ydfG"
                     /locus_tag="ACHKFK_004571"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO9054751.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASIEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4091..6136
                     /gene="dcp"
                     /locus_tag="ACHKFK_004572"
     CDS             4091..6136
                     /gene="dcp"
                     /locus_tag="ACHKFK_004572"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO9054752.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGIQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLEQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNNWL
                     IPLLNTTQQPALAELRDRATREKLFTAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATNPQVFARYARHYQSRAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGRRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNV"
     gene            complement(6181..6261)
                     /locus_tag="ACHKFK_004573"
                     /pseudo
     CDS             complement(6181..6261)
                     /locus_tag="ACHKFK_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6285..6389
                     /locus_tag="ACHKFK_004574"
                     /pseudo
     CDS             6285..6389
                     /locus_tag="ACHKFK_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00156 family protein"
BASE COUNT         1624 a         1519 c         1645 g         1660 t
ORIGIN      
        1 tccgataaaa ttggtcgccg gatcccgtat attattatga atacctctgc gattgtgctg
       61 gcatggccaa tgctttctat catcgtagat aaaagctatg ccccgagcac cattatggtt
      121 gcactgattg tgattcataa ctgtgcggtg cttggattat ttgctctgga aaacattacc
      181 atggcagaaa tgttcggctg taaaaaccgc tttacccgaa tggctatttc taaagaaatt
      241 ggtggtctta tcgcttccgg ttttggtcct atcctggcgg ggattttctg caccatgacg
      301 gaatcctggt atccgatcgc cattatgatc atggcatatt ctgtgattgg tttaatctct
      361 gcgctgaaaa tgccagaagt gaaagaccgt gatttaagtg cgctggaaga cgccgcagaa
      421 gatcaaccac atgttgtaag agctgcgcaa ccgtccagaa gtctgtaaat cctcaatccc
      481 ttctcttacc ggagagggga tttttattca tataacaaaa catgtagctt gctatattta
      541 attttaagga attgttatgg gaaatcattt gttatcagca aaagcgacgc tccctgttta
      601 tgatcgtaat aacctggccc caagaattgt tcatttaggc tttggtgcat ttcaccgtgc
      661 acatcagggc gtgtatgccg atattcttgc tacggaacat ttcagtgact ggggatatta
      721 tgaggtcaac ttaatcggcg gcgaacagca aattgccgat ttacaacagc aagataatct
      781 ttataccgtt gcggaaatgt cggctgatgc gtggacggct cgcgtcgttg gcgtcgttaa
      841 aaaagccttg cacgtacaaa tagatggctt agaaaccgtg ttggctgcga tgtgtgaacc
      901 gcaaatcgcg attgtctctc tgacaatcac cgaaaaaggg tatttccact ctccggcgac
      961 cggacagtta atgctcgatc acccgatggt cgttgccgac gtgcaaaatc cccaccagcc
     1021 gaaaactgca acaggggtga ttgttgaggc gctggctcgc cgtaaagcgg caggacttcc
     1081 cgcatttacc gtcatgtcat gtgacaacat gccagaaaac ggtcatgtta tgtgtgacgt
     1141 tgtcacttcc tatgcgcaag ccattgatgt aaaactggca caatggatcg aagataacgt
     1201 gactttccca tcaacaatgg tggaccgtat tgtgcccgca gtgacagagg atacgctggc
     1261 gaaaatcgaa caacttaccg gtgtgcgcga tccggcaggc gttgcctgtg aacctttccg
     1321 ccagtgggta atagaagata actttgttgc cggacgtccg gaatgggaaa aagcgggagc
     1381 cgaactggtt agtgatgtgc tgccttatga agagatgaag ttgcgcatgc ttaatggcag
     1441 tcattcattc ctggcgtatc tggggtatct tgccggatat cagcacatta atgactgcat
     1501 ggaagatgaa cattatcgtc atgcagcgta tgccttaatg ttgcaggaac aagtgccgac
     1561 gctgaaagtg cagggcgttg atttgcaaga ttacgctaac cgattaattg aacgctatag
     1621 caacccggcg ttacgtcatc gaacctggca gattgcgatg gatggcagcc agaaattgcc
     1681 acagcggatg ttggattctg ttcgctggca tctggcgcat gacagcaagt tcgatctgct
     1741 ggcgctgggc gtcgcgggtt ggatgcgtta tgtcggtggt gttgatgaac agggaaatcc
     1801 gatagaaatc agtgatccac tgttacctgt tattcagaag gcagtacaaa gtagtgctga
     1861 agggacagcg cgcgtccagt cactgctggc aattaaggcg atctttggtg atgatttacc
     1921 aggcaatagt ttgtttacga caaaagtaac ggaagcgtac ttgtctttat tagcgcatgg
     1981 cgcgaaagcg accgtggcga aatattccgt gaagtaaaaa aagcaatgcc gccatcggcg
     2041 gcattcacac gcttagttca tgccgagacg aatcaggtcc agtggtgcca gtttctcttc
     2101 acaaccttca acaactacgg tttttccgcg gcggatttgc tcagcggtca gaccttcagt
     2161 atcaatcgac aggatcttgc cagtgtgacc ggtgccgcta accataacac ggctgccagt
     2221 ggtgattgcg ttacggttac gatcgtaggt cgtcataggt gttttctcct ttctgattta
     2281 cagtcgaagc ccacgtaacg ggcgcaatat aaatactcct gtcgagtgaa atattttttg
     2341 tttttgatca agctcacatc ttttttgatg catgcatgag ccataagtcg ggggaaaaga
     2401 ctctcacccc ggatgggatg agaggaaatg aattactctt cgctaaacca gtcgctgttt
     2461 tcctggcgga tctggcgcac agattcgctg atttcctgca aatgttgcgt cattgcacgt
     2521 tctaccgcat cgccatcacg tttttgcagg gcagagaaaa tatcaagatg ctggcgtaac
     2581 agcatttctg gtggagaaac gtggtcgaaa ctcatatagc gcacgcgatc aacggtcgct
     2641 ttcaggttct caatggtatc ccacgccagt tgacagtcgg caatctgcgt caggagttga
     2701 tggaagttgt catcaagttc aaaaaaatca tccagttgct tgcgctcaat ggcaatgcgt
     2761 tgctggtgaa gattttgttc cagttggtag cactggcttt cggtaatcat gctcgccgcc
     2821 cgacgcgcca ccgcgcactc aatggcctga cggataaaac tgccgttgcg cacctgggcc
     2881 atggaaattt tgttgacgta gctgccacgc tgcggacgaa tttgaatcag accgttttcc
     2941 gccagtttaa taaaggcttc acgaaccggc tggcgtgaca cattgaaacg aacagaaact
     3001 tctttttccg acaacggtgt gcctggtgca atcaggcaat ggacaatgtc gcggcgaaga
     3061 atacgataaa tctgctgatt gacaggctgt gtgggattaa gttgcgtttc gacggtcatt
     3121 cgttcttgct tgtgagtgag ttaactgcat gagtctacca cttttttgtg acaagcaata
     3181 acccggcagt tacgccgggt ataaaaatta ctgacggtgg acattcagtc cggcatagct
     3241 ttgggtaacc ggcatcattt ccagggtatt gatattgacg tgagcaggca gcgttgacac
     3301 ccaccagacg gcttcgctga catcttctgg tgtcaatgca acggtatttt gataggtttt
     3361 ttccgcttta ccatcatcgc ctttaaagcg gacattggaa aactcggtgc cacccaccag
     3421 acccggttcg atgtcggtga cgcgcaccgc cgtaccatgc aggtccgtac gcagattcag
     3481 gctaaattga cgaacaaacg ctttcgtcgc accgtaaacg ttaccaccgg catacggcca
     3541 gctaccagcc gttgagccaa tgttaataat atgaccatga ttacgttcaa ccatacccgg
     3601 taagacggcg cgcgtcatat ataccaggcc tttgttgttg gtatcaatca tcgtttccca
     3661 gtcttcaatg ctggctttat gcgcaggctc catgcccaac gccaggccgg cattgtttac
     3721 caggatatca atattgctcc actcggcagg gagcgatgcc agcatctctt caatagcggc
     3781 gcggttgcga acgtccagtt gggcgatata cagattatct cccagttcgt cttttaactc
     3841 ctgtaaccgc tcctggcgac ggccagtggc gataacttta tgcccttgtt gaataaaacg
     3901 acgagtaatg cattcaccaa aacctgccgt tgctccagtt actaaaacga tcatctcact
     3961 gttcctcaac gcttttgtgt gtcaataaca tagcacgtga tgcttaagag cgggtaatac
     4021 tggtttgcaa gattgcactc cgttatcagt aagcctacgc tatggaacac ctcgcgttca
     4081 ggagaagaaa atgacaacaa tgaatccttt ccttgtgcaa agcacactgc cgtatctggc
     4141 tccccatttt gatcaaattg ccaaccatca ctatcgcccg gcattcgatg agggaataca
     4201 gcaaaagcgg gcagaaatag ctgccatcgc gcttaacccg caaacacctg atttcaacaa
     4261 tactattctg gcactggaac aaagcggaga attacttacc cgcgttacca gcgtcttttt
     4321 tgcgatgact gcggcgcata ccaatgatga attacagcgt cttgatgaac agttttccgc
     4381 tgaactggcg gaacttgcga atgatatcta tctgaacggg gaattattcg cgcgggtaga
     4441 tgctgtctgg caacgccgtg aatccctggg gcttgatagt gaatccatcc gcctggtgga
     4501 ggtgattcat caacgttttg tccttgccgg agccaaactt gagcaagctg ataaagcaaa
     4561 attaaaagta ctgaatacag aagctgcgac cctgaccagc cagtttaacc agcggttact
     4621 ggcagcaaat aaatccggcg gtctggttgt gaacgatatc gcgcagctgg caggaatgag
     4681 tgaacaagag attgcgctgg cggcagaggc ggctcgcgag aaaggtctgg ataacaactg
     4741 gctgattccg ctgctgaata ccacccagca accagcgctt gctgaactgc gcgatcgcgc
     4801 gacgcgtgaa aaactgttta ctgcgggctg gacgcgagcg gaaaaaaatg atgccaatga
     4861 tacccgcgct atcattcaac gtctggtgga gatccgcgcg cagcaggcga aactgcttgg
     4921 ttttcctcat tatgccgcat ggaaaatcgc cgatcagatg gcaaaaacgc cagaagcagc
     4981 gctcaacttt atgcgggaaa ttgttcctgc ggcgcgtcaa cgtgcgagcg atgaattagc
     5041 ctccatacag gcggttatcg ataagcagca gggcgggttt agcgcgcagc cgtgggactg
     5101 ggcattttat gccgagcagg taagacgtga gaaattcgat ctcgatgaag cgcagctcaa
     5161 gccttatttc gaattaaaca cggtgttgaa tgaaggcgta ttctggactg cgaatcagct
     5221 cttcggtatt aagtttgtcg aacgttttga tattcctgtc taccatcctg acgttcgtgt
     5281 gtgggaaatt tttgatcata atggcgtggg gctggcgtta ttttacggtg atttcttcgc
     5341 ccgtgattca aaaagcggcg gcgcatggat gggcaatttt gttgagcaat caacgctgaa
     5401 tgaaacgcac ccggtaattt ataacgtctg caattatcag aaacccgctg ccggtgagcc
     5461 tgcgttgtta ctctgggatg atgtaataac cttattccat gaatttggtc atacgctgca
     5521 cggccttttt gcccgccagc gttatgccac gctttccggc accaacacgc cgcgtgattt
     5581 tgtcgaattt ccgtcgcaaa tcaacgaaca ctgggcaaca aatccgcagg tattcgctcg
     5641 ctacgcccgg cattatcaga gcagagccgc aatgcctgac gaacttcaac agaaaatgcg
     5701 taatgccagc ctgttcaata aagggtatga gatgagcgaa ctgcttagcg ccgcacttct
     5761 cgatatgcgc tggcattgcc tggaagaaaa cgaagcaatg caggatgtcg atgatttcga
     5821 attgcgggcg ctggtggcgg aaaatatgga tcttcctgct ataccgccac gctatcgcag
     5881 cagttatttc gctcatattt ttggtggcgg atatgccgca ggttattacg cttatctgtg
     5941 gacgcaaatg ttggccgatg atggttacca gtggtttgtt gagcagggcg gattaacgcg
     6001 tgaaaatggg cggcgttttc gcgaggcgat tctttccaga ggtaacagcg aagatctgga
     6061 acgcctgtat cgacaatggc gcggtaaggc acctcagatt atgccgatgc tgcaacatcg
     6121 tggcttgaac gtataaacac aaccaatggc tggcctggat ttatcgccag accagcctcg
     6181 ttatagcagt aattgcagca gctgggcgag ggcaaacttc accgctaatg cgaatgccgt
     6241 ttcgcagtcg ccagcaaaat tacttttttt gtataccctt ccgttataat aattttcaaa
     6301 ttcagccgga ccagatgagc aacattagcg gcagtctgga taagaaaagc gcgccgaccg
     6361 tcgttcgggt cactcatttg cagaaataaa aataaattag cctgatggcc tgaactactg
     6421 ggccattagg gtattgggtt atgctatt
//
LOCUS       DAZGJT010000178         6156 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZGJT010000178 DAZGJT010000000
VERSION     DAZGJT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.182"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..143
                     /locus_tag="ACHKFK_004575"
     CDS             54..143
                     /locus_tag="ACHKFK_004575"
                     /inference="COORDINATES: protein motif:HMM:NF017787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO9054753.1"
                     /translation="MTAYRNVFIQDDPGMHFRRVIRNAEGQRR"
     gene            322..1143
                     /locus_tag="ACHKFK_004576"
     CDS             322..1143
                     /locus_tag="ACHKFK_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO9054754.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVF
                     DRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
     gene            1198..1683
                     /locus_tag="ACHKFK_004577"
     CDS             1198..1683
                     /locus_tag="ACHKFK_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO9054755.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATLVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1699..2175
                     /gene="radC"
                     /locus_tag="ACHKFK_004578"
     CDS             1699..2175
                     /gene="radC"
                     /locus_tag="ACHKFK_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO9054756.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            2262..2483
                     /locus_tag="ACHKFK_004579"
     CDS             2262..2483
                     /locus_tag="ACHKFK_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO9054757.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            2646..3014
                     /locus_tag="ACHKFK_004580"
     CDS             2646..3014
                     /locus_tag="ACHKFK_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO9054758.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            3104..3478
                     /locus_tag="ACHKFK_004581"
     CDS             3104..3478
                     /locus_tag="ACHKFK_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO9054759.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKHPEKR"
     gene            3475..3963
                     /locus_tag="ACHKFK_004582"
     CDS             3475..3963
                     /locus_tag="ACHKFK_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO9054760.1"
                     /translation="MKLSLMLEADRINVQALNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVEESDQQSTCTLPPFATLAGIRCSTAHITEKDNAWLYSLSHQTSDVGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVITLTRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            3975..4172
                     /locus_tag="ACHKFK_004583"
     CDS             3975..4172
                     /locus_tag="ACHKFK_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO9054761.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            4257..4406
                     /locus_tag="ACHKFK_004584"
                     /pseudo
     CDS             4257..4406
                     /locus_tag="ACHKFK_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit M"
     gene            4382..4510
                     /locus_tag="ACHKFK_004585"
                     /pseudo
     CDS             4382..4510
                     /locus_tag="ACHKFK_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            4848..6045
                     /locus_tag="ACHKFK_004586"
                     /pseudo
     CDS             4848..6045
                     /locus_tag="ACHKFK_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002441793.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1541 a         1550 c         1546 g         1519 t
ORIGIN      
        1 cagagtatgt cctggctgaa ggatctttct catacgggca ggcggttgct gtcataaccg
       61 cttaccgcaa tgtgtttatc caggatgacc cgggaatgca ttttcgtcgg gttatccgca
      121 acgcggaagg tcagcgacgc tgacgcagcc ggaactctga agctgatgcc ggaaaacagc
      181 ttaatgcgtg gctcgccagt ggagggcttc tcagacaata aacctcattg atttcatcca
      241 tcaggccgcg tcttctccgg gagacgcggc ttttttcatt tattacacca gtaaatctta
      301 accaccgata aggagcaaag tatgcgatta gccagtcgtt ttggttatgc tgcaaaccag
      361 atacgtcgtg accgcccact gacacatgaa gagctgatgc accatgtgcc ggggattttc
      421 ggggaagata aacacacgtc ccgaagccag aactatacgt acatccccac tatcaccgta
      481 ctggaaagcc tgcagcggga aggttttcag ccctttttcg cctgccagac ccgtgtgcgc
      541 gacccgggcc gccggggata cacaaaacac atgctgcgtc tgcggcgggc cggagagata
      601 aacggagaac atgtccctga aattattctg ctcaactctc atgacggtac ctccagctac
      661 cagatgctgc cgggttactt caggttcgtc tgccagaacg gatgcgtctg tggtcagtct
      721 ctgggggaag tgcgtgttcc gcaccgggga aatgtggtgg acagagtcat tgaaggggct
      781 tacgaggtgg tgggcgtgtt tgaccggatt gaggaaaagc gtgatgccat gcagtcgctg
      841 attctgccgc caccggcacg ccaggcgctg gcacaggcgg cactgactta ccgttatggt
      901 gacgaacatc agcccgtcac caccgccgac attctgacgc cacgacgccg ggaggattac
      961 ggtaaggacc tgtggagtgc ttatcagacc attcaggaga atatgctgaa aggcggaatt
     1021 tcaggtcgca gcgctaaagg aaaacgtatc catacccgtg ccattcacag cattgacact
     1081 gacattaagc tcaaccgcgc attgtgggtg atggcggaaa ccctgctgga gagcctgcgc
     1141 tgattcagcc attaccctga aaaccacgca tttatttgtc ctttaaggag tcatttcatg
     1201 aaaaccattt cacataattc caccacatct tctgtttccg taaccgctgc atcagggaat
     1261 gaccagcctc agttggttgc caccctcgtc cctgatgaac agcgcatcag cttctggccg
     1321 cagcactttg gcctcattcc acagtgggtg accctggagc cccgtgtctt cggctggatg
     1381 gaccgtctgt gtgaagacta ctgcggtggt atctggaatc tgtacaccct gaacaacggc
     1441 ggggcattta tggcacccga accggatgac gatgatgacg aaacatgggt actgttcaat
     1501 gccatgaacg gtaaccgcgc tgaaatgagt ccggaagctg ccggcattgc tgcctgtctg
     1561 atgacgtaca gtcatcatgc ctgtcgtacg gagtgttatg ccatgacggt ccattattac
     1621 cggctgcggg attacgccct gcagcatccg gaatgcagcg ccattatgcg catcatcgac
     1681 tgaatggaga gaagcacaat gcaacaactt tcctttctgc ccggagagat gacgcccggc
     1741 gagcgcagtc tcattctgcg ggccctgaaa actctggacc gccatcttca tgaacccggc
     1801 gtggccttca cctccacccg tgcggcacgg gaatggctga ttctgaacat ggcgggactg
     1861 gagcgtgaag aattccgggt gctgtatctg aataaccaga atcaactgat tgccggtgaa
     1921 accctcttca ccggcaccat caaccgcacg gaagtccatc cccgggaagt gattaaacgc
     1981 gccctgtacc acaatgccgc tgccgtggtg ctggcgcaca atcacccgtc cggtgaagtc
     2041 acacccagca aggcagaccg ccttatcacg gaacgtctgg tacaggcact ggccctggtt
     2101 gatatccggg taccggacca tctgatagtc ggtggcagcc aggttttctc ctttgccgaa
     2161 cacggtctgc tttaacccgt taccgtcaca atcaccttca tatcacttca gtttctcttt
     2221 ctcagctgtt tcttactttc acattcagga ggactattct catgaaaatc atcacccgtg
     2281 gtgaagccat gcgtattcac cgtcagcatc ctgcatcccg tctttttccg ttctgtaccg
     2341 gtaaataccg ctggcacggt agtgctgaag cgtataccgg tcgtgaagtg caggatattc
     2401 ccggtgtgct ggccgtgttt gcagaacgcc gtaaggacag ttttggtccg tatgtccggc
     2461 tgatgagcgt caccctgaac tgatgttccg tcacccggaa aacgagtacg actggctgag
     2521 ctcagacgcc gctgtggatg aggtcctgct catcaacgaa tacaccttta ccgaagcccg
     2581 tctgcgcgcc ggctgacccg tcatcgttaa actatcattc accgattttc tgtgaggatt
     2641 ttatcgtgtc agacacactt tccgggacaa cgcatcccga tgacaatgac gatcatccct
     2701 ggtggggact accctgcaca gtgacgccct gtttcggcgc ccgtctggtg caggagggta
     2761 accgcctgca ttaccttgcc gaccgcgccg gtatcagagg cctgttcagc gatgcagatg
     2821 cgtaccacct ggaccaggcc tttccgctac tgatgaaaca actggaactc atgctcacca
     2881 gcggtgaact gaatccccgc catcagcata ccgtcacgct gtatgcaaaa ggtctgacct
     2941 gcaaagctga cactctcggt agcggtggtt acgtttatct ggctgtttat ccgacgcccg
     3001 aaacgaaaaa gtaaaccacc ggaataacct tctgctccgg cctggtgctt tcaccacgcc
     3061 acttttccat ttttcatctc tgcatatcag gaaaaccttc agtatgaaaa cattacccga
     3121 cacacacgta cgggaggcat cgagctgccc gtctcccgtc accatctggc agactctgct
     3181 ctcccgacta ctgggccagc attacggcct tacactgaat gacacaccgt tcgccgatga
     3241 acgtgtgatt gagcagcata ttgaagctgg catttcactg tgtgatgcgg tgaattttct
     3301 cgtggaaaaa tacgcgctgg tgcgtaccga ccagccggga ttcagcactt gtccccgctc
     3361 tcagttaata aacagtattg atatcctccg ggctcgccgg gcgaccggcc tgatgacccg
     3421 cgacaattac agaacggtaa ataacattac cctgggtaag catccggaga aacgatgaaa
     3481 ctttccctga tgctggaagc cgacagaatt aatgtgcagg cactgaacat ggggcgaatt
     3541 gtcgttgacg tcgatggtgt taatctcact gaactgatta acaaggtcgc tgaaaacggt
     3601 tattcactcc gcgtggtgga ggaatccgac caacagtcaa cctgcacact accaccgttt
     3661 gcaacccttg ccggcatacg ctgcagtacc gcacatatca cggaaaagga taacgcctgg
     3721 ctgtactcgc tgtcgcatca gaccagtgac gttggtgaat cagaatggat tcatttcaca
     3781 ggtagcggat atctgttacg taccgatgcg tggtcatatc cggttctgcg gcttaaacgc
     3841 ctggggctgt caaaaacgtt ccgtcgtctg gttatcacac ttacccgacg ttatggcgtc
     3901 agtctcattc atctggatgc cagcgctgaa tgcctgccgg gtttacccac tttcaactgg
     3961 taaccaggaa caacatgaaa tcattaacca cggaaaccgc actggatatt ctgattgcgt
     4021 ggctgcagga caatatcgac tgcgaatcgg gaattatctt tgacaacaat gaggataaaa
     4081 cggattcagc agcactgttg ccctgtatcg aacaggccag agaggatatc cgtaccctgc
     4141 gccaactgca gcttcagcac cagaaccggt gagtcttact caccagactt cattccactg
     4201 acgccagcct gaacagggct ggcattttca tttatctgca aaaaggaata tcgattatgt
     4261 ctgatatcac agcctaccgt ccggaagtgg tcaacgagaa tacggacgtt atctgctcaa
     4321 ccagcatccg ccgcattctg gctgtacgaa agagttcact gctgcaaatc gacacaatta
     4381 tccggcatca agtgagcgat atcagttaac agatatagat ggagaagatg cttttatccg
     4441 gattggttct atcgcgatgg tgcgagtact acttgatgca atgaagcctg tcgaggaaga
     4501 tgacgagtaa cagagctcgg tatctcaatc aggagctgat tgtcctggtt cgatgccgaa
     4561 aaccagtgat ttttcgtgtt ggtatctgtg ttggtataag atgatattgt ttattattaa
     4621 caattttaaa tcatgaggtt aagtcacatg tttgagtccg gccttcgcac caatcaaggc
     4681 tttttcagtc tacttgtgta tataaatcaa taagttagat aatatccctc ttgtttcagc
     4741 tccattaaag tctactgtcg tatatcaata tctatcaata aatgttggta taactcttgg
     4801 tggcttctgt tcgatttata ttgatgccaa catagcgagg gggatgtatg gctcttactg
     4861 atatcaaagt cagaacggtt aagcctgcag acaaatcttt caaacttacc gatgatgagg
     4921 gggatgcacc tgctggttaa actaacccca atggcccaaa atactagcgt cttcaatacc
     4981 gtttaagtgg caaacagaag atgctggcgt tagggggtta tctatggcct cattggcaga
     5041 ggcttgtaag aggcacgatg cagcaaagaa actggtatct gatggcatcg atccctctca
     5101 aaagaaaaag aggaaaagat tgcagaaagc ggagcgctaa cctttgaggc tgtggtaaga
     5161 gattggcagg catcctgtag taaaaaatgg tcggagcccc atagtgaacg agtgcttaaa
     5221 atcttagtgg ataatctttt cctggcgtta agtaaacgta aaatttcgga actgaagaca
     5281 cggggtctac tcgtctttat aaagatagtt gaggtgagcg gaccttatga agttgtctaa
     5341 cgtcttcaac aaaatacaac tgctatcatg catttcgcgt tacaaaatgg tctgattgat
     5401 tataacccgg cccaagacat agctggtgcg gtagccgtgg ctaaacgagt ggatcgtcca
     5461 gggtttgatt aagaacgtct tcccgaatgg cttgatcgca tcgaatgctt taaaggtcga
     5521 aagtttacta aattggcagt gaaactcata ctgctggtat tcatacgttt aggtgaactg
     5581 cgttttacaa gatgggaaga aatcgatttt aaaaatgcac tttggacgat acctgcacaa
     5641 cgagaacctc tgaaggcgta aagcacgcac attgtggttc taagatgcgt agtccttatc
     5701 ttgtactatg aagccgtcag gcactggaaa tgttaggaga gataaaacaa accagtggtc
     5761 ttcataggcg ttcaccagcc ggttaggcag atgagtgaga acacggttaa taaggccctg
     5821 cgcttaatgg gctacgagat tacaacggaa gtttgtggac atggggttag ggcgatggcc
     5881 tgtagtgcgt tgatcgaatc aggtttatgg tcaggggatg ctgttgaacg tcaaatgagc
     5941 catcgggagc gtatcaatgt ccgaacttct tctatccatc ttgctgagca tcttgatgcc
     6001 aaccgagtca gaacggttag gtcgtttgag tttgcccaca gctgatcgtt ttgagtttga
     6061 aaatcaaagt taaccgcttg ataacctatc acataatagc atgtaactct ttgttttgaa
     6121 tatatttgag tagccatcaa ttgttgatgg ctacta
//
LOCUS       DAZGJT010000179         6130 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZGJT010000179 DAZGJT010000000
VERSION     DAZGJT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.183"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            13..3342
                     /locus_tag="ACHKFK_004587"
     CDS             13..3342
                     /locus_tag="ACHKFK_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009485633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO9054762.1"
                     /translation="MSNVLLFLVIVAFVGGIIWRFGDHFGLKGDENNFFAWLICIMTL
                     IFVRASWYFSRLYRLRKEQHHSEKERLRPEKEARIEYGKVITLNIELAEYLCALYGPF
                     WRRKIRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLAALKKL
                     RRSRPLDGIIWPLTEEQSRQTAQLDKGWRELINGSKRLGFQAPLYLWQVCDNGDYQTG
                     RPLQSVGCLLPERCNPEQLAAMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAERG
                     IAHWQSILKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSPVWAGVTGDNARG
                     RTVGFPWLRTALIIGICILAIWGAGMTTSFFANRALVQETGIQTARALDTRLPLSEQL
                     VALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVDH
                     LQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRYE
                     NIPEGVRQSVLPSLLTFWTANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLY
                     QNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTA
                     RREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIRWKKEDSLSG
                     ILDQLTLMADARQSPLIALTDTLAWQAATGRENRGLSDSLAKSAQELFNGKEKTPQQS
                     REGNEPVGPLDKTFAPLLRLLGDKAGGGGGDTQLSLQTYLTRVTRVRLKLQQVTNAPD
                     PQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVL
                     TPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAAN
                     LSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKP
                     ARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRG
                     SWSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPG
                     QVFLTGRSMKDAEEYGEDADE"
     gene            3335..4945
                     /gene="tssA"
                     /locus_tag="ACHKFK_004588"
     CDS             3335..4945
                     /gene="tssA"
                     /locus_tag="ACHKFK_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO9054763.1"
                     /translation="MSELLQKLTRSCFADRDALDVALTQAALWQTWLLPVTADTPVGE
                     DPGYHDDFLRIRDEMNKLSGTDTGLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKDDFANI
                     VAALNQLTVSFAAWPEDQHSPSLMPLINALESRLAQSGGMNAVVPQNSSGVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSSGVSHFWLDIQWYLHQALAKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDGTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDIATQGITLTQWEPELLFEVKARQLKLLRLRAHRYADKALLN
                     RKMDALLGTLVAINPVRAAVLCDTQHKE"
     gene            4945..>6130
                     /locus_tag="ACHKFK_004589"
     CDS             4945..>6130
                     /locus_tag="ACHKFK_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO9054764.1"
                     /translation="MTMGRGYFLVRGDKTTCGGKIIEGADDHTIMGIPQARDMDRMTC
                     GKHPGMFIIVGGVPETGIHGRLMAGSLDSQSSCPCKARFIASMMDDVYEVETEKTENK
                     SVMPLNPLNNGTGNSSPPAYTSDYPALRNTHNLPDQQVRDMLANNNHDVMLLTYEEGA
                     EVLQSWGCQDFKRKWVDITQSAPGQLIINYGVNGKDVVTTSIVLARLGDFGIKATAYV
                     NHKGTELIKLTGYPGIRKILNAPVFAAKNPKIVDVGIGKYGLTNSIVKGARLTFYVAA
                     AYRTIDFILNDETSLAEFIGALATDVGKIAIASAVSWGAGKALLATGFVAGPLVGVVV
                     VGIGAALLLNYLDNKFGLTDQVVAYIEAAQQEFVEKAREIEEGIWDLGAMWAKHMLIK
                     GKE"
BASE COUNT         1388 a         1635 c         1883 g         1224 t
ORIGIN      
        1 aaaagtgttt ttatatctaa tgtactttta ttcttggtta ttgtggcctt cgtggggggg
       61 atcatctggc gttttggtga tcattttgga ctgaaagggg atgaaaataa tttttttgcg
      121 tggttgatat gcattatgac cctcattttt gtacgggcat cctggtattt ctctcgattg
      181 tatcgcctac gaaaggaaca acatcactcc gaaaaggaaa gattacgccc ggagaaagag
      241 gctcgtattg aatatggcaa ggtcataacc ctgaacattg agctagctga gtatttatgt
      301 gccctatacg gcccattctg gcggcgtaaa atccgcctgc tgctggtgac cggcgagcct
      361 gaacaggcag aggccatcgc gccggggctg accgggcaac actggctgga gggcgaccac
      421 acggtgctga tatatggcgg caggccatca gcggagcctg atgtcacact gctggccgcc
      481 ttaaaaaaac tgcgccgcag ccgtccgctg gacggcatca tctggccgct gacagaagaa
      541 caaagccgcc agacagcgca actcgacaaa ggctggcgcg aactgataaa cggcagtaag
      601 cgactcggtt ttcaggcccc actctatttg tggcaggtct gtgacaacgg tgattatcag
      661 accggacgcc ccctgcaaag cgttggctgt ctgctgccgg aacgctgtaa cccggaacaa
      721 ctggctgcaa tgctggaagc acaaaccctg ccgctgacgg aacagggcat gtcgcagctg
      781 ctgaccgata accgccatga tttcctgctg cgtctggccc atacccttgc agagcggggt
      841 atcgctcact ggcaaagtat cctgaaaccg ctgctggcag gcggcgcatt ttcttccctg
      901 cgcctgcgtg gcctgatgtt cagcccgccg cttaccgccg tgccggaggc cgcacctcat
      961 gcgtggctgc cgtcgccggt ctgggcgggc gtgacgggtg acaacgcacg gggtcgcacg
     1021 gtgggctttc cgtggctgcg taccgcgctg ataatcggca tctgcatact ggcgatatgg
     1081 ggggccggaa tgacgacctc gttcttcgcc aaccgcgcgc ttgttcagga aaccggtatc
     1141 cagacggcac gtgcgcttga tacccgcctg ccgctgtcag aacaactggt ggcgctgcat
     1201 accctgcagg gcgaactgga gcgcctgcaa taccgtatcc gtgagggtgc gccgtggtat
     1261 cagcgttttg gccttgaacg taaccagcaa ctgctcgctg ccgcttttcc cggctatgcg
     1321 caggcggcaa accggctggt gcgcgacgtg gccgttgacc atctgcaaca gcaacttaat
     1381 gcctttgtcg cgctgccgcc caacagtcct cagcgtaccg ccaccggtga acaacgctat
     1441 aagcagctta aggcattgct gatgacttcc cgcccggaaa aggccgacgc tgcctttttc
     1501 agtaccacgc tgatggcgga cggtctgcgc tacgagaata tcccggaagg tgtgcggcag
     1561 agcgtgttgc cgtcactgct gaccttctgg acggcgaacc tgccggaaca cccgcagtgg
     1621 aaaacatcgc cgccaccgga actgaccggc gcagtgcgta aaatcctgct gcgccagatt
     1681 ggtgtgcgta atgccgaaaa caccctctac cagaacgtgc tgcaacaggt gtcccgcaac
     1741 tacgccgata tgacgctggc ggacatgacc ggggataccc tcaccgaatc tcttttcagt
     1801 acggaacaga cggtgccggg gatgttcacc cgtcaggcgt gggaaggaca ggtcagggaa
     1861 gccatcgagc aggtggtgac ggcgcggcgc gaggaaatcg actgggtact cagcgaccgc
     1921 cagcaggata cctctgcgga tatctcgccg gatacgctgc gtaaccgtct cacctcacgc
     1981 tactttaccg actttgccgg aagctggctg gcgtttctca acagcattcg ctggaaaaag
     2041 gaagactcgc tctccggcat tctcgaccag ctgacactga tggccgatgc ccgtcagtcg
     2101 ccactgattg cgctgacgga caccctcgcg tggcaggcgg cgacaggcag ggaaaaccgt
     2161 ggcctgtcag actcgctggc gaaatcggca caggaactgt ttaacggcaa ggagaaaacg
     2221 ccgcagcaat cccgtgaagg caacgagcct gtcgggccgc tggataaaac cttcgcgccg
     2281 ctactgcggt tgctgggcga taaggcagga ggcggtggtg gtgatacgca actcagcctg
     2341 caaacgtatc tgacccgcgt cacccgcgtg cgtctcaaac tgcaacaggt gaccaacgcc
     2401 cccgacccgc aggagatgac ccagcaactg gcgcagacgg ttttacaggg caaaaccgtt
     2461 gacctcaccg acacccgcga ctacggacgg ttaatcgccg ccagtctggg cgaggaatgg
     2521 agtggcttcg gtcaggcgct gttcgtccgc ccggtggagc agtcgtggcg gcaggtgctg
     2581 acgcctgcgg cggacagcct gaaccgtcag tggcagcggg cgattgtcag ccactggaat
     2641 caggacttcg ccgggcgcta tccgttcaaa gcctcacaaa acgatgcctc tcttcccctg
     2701 ctggcacagt atctgcgcga tgacgggcgc atcaaccagt ttatcgccgc caacctttcc
     2761 ggtgttctga aacgtgaagg ccgctactgg gtggctgacg ccatgaacac gcaggggctg
     2821 acggttaacc cggactttat ccgcgccctg aaccgcctgc gtgacgtggc cgacaccgcc
     2881 tttgccagcg gcgatgcggg aatacatttc gaactgcggg caaaaccggc gcgtgacgtg
     2941 atgaaaacgc atctggtgat tgacgggcag gagctggaat atttcaacca gaaggaacgc
     3001 tggcagcgtt tcaactggcc ggatgaacag tggcaacccg gcgcatcgct gagctggacc
     3061 agcacgcagg cgatggaacg cattctggcg gattaccggg gaagctggag ccttattcgc
     3121 ctgctggaac aggcgcaggt gacaccggtg gacagcagca cctttaaggt ggtgtggaaa
     3181 gcgcaggatg gcctgccgct gaattacctg ctgcgggttg aacagggtaa agggccgctg
     3241 gcgctgctgg agctgaaaaa cttccgcctg ccgggacagg tatttctgac cggcaggagt
     3301 atgaaggatg cggaagagta tggggaagac gccgatgagt gaactgttgc aaaagctgac
     3361 ccgtagctgt tttgccgacc gggatgcact ggatgtggcg ctaacacagg cggcgctgtg
     3421 gcagacatgg ctgctgccgg tgacggcaga cacgccggtc ggggaagacc ccggctacca
     3481 cgacgatttc ctgcgtatac gcgacgaaat gaacaaactt tccggcacag ataccggcct
     3541 tatctgccag cttgccgaat ccctgctgct gacgcaggcc aaagacgtgc gcatcgccac
     3601 ttactacatc tgggcgcgac tgcaccgcga cggtgagcgc ggactggcgg aaggactagc
     3661 gctgctgacc ggactggtgg aacgcttcgg tacgcaactg ctgccctcgc gaccggccag
     3721 caggaaaatg gcgctggagt ggctggcggg ggaaaagatg ctcgacagcc ttgcccgcta
     3781 cccggaagtg gcaaaagatg attttgcgaa cattgttgcc gctcttaatc aactgacggt
     3841 ttccttcgcc gcatggcccg aagaccagca ttccccctcc ctgatgccgc tgattaacgc
     3901 gctggaatcg cgtctggcac agtcgggcgg aatgaacgcg gtggtgccgc agaacagcag
     3961 cggcgttccg gcaccgtcat ccccggtgga tgcgccgcag gtacagacca tcacctccgg
     4021 acgtgacctg ctcgaccagg cgaaagtgct ggcccgttat ctgaacgaac agccgcaggg
     4081 ctggctttcg gcgcaccggc tgatgaaaac cctgcgctgg gatacggtgc atgaactgcc
     4141 gccggacgtg gacggcaaaa cccggctggc cccaccgcgc accgagtcac gcaaccagct
     4201 taagcggctg tatgcccagc agaactggac ggaactgctg gagcaggccg acctgatgtt
     4261 cagctccggc gtcagccatt tctggctgga tattcagtgg tatctgcatc aggcgctggc
     4321 taaggcgggc gcaccgtggg acagatggac agcggttatc cgtcaggacc tggcgctgct
     4381 gctggagcgc ctgccggggc tggaaaatct ggcatggaac gacggcacgc cgttcgccga
     4441 tgaggtgacg cgcaactgga tagcgcagca ggtcatgatg cgggaagacg gcgcatggct
     4501 tgcggggaaa gccgctgtac cgacagacga cgccacaaac gacgtactgg cgctggagcc
     4561 ggaagcactg gagatggcag acagtcaggg cgtggaagcg gcgctgggct ggatacagac
     4621 ccgtccgggc atcaccacgg cacgccagcg cctgttattg cggctgctga tggcgcgggt
     4681 ggcggaacag tacggtaaaa acgagatggc gctcctgctg cttgaagaac tggatattgc
     4741 cacacaggga ataacgctga cgcagtggga gccagagctg ctgtttgagg taaaagcccg
     4801 acaactgaaa ctgctgcgtc tgcgggcgca ccgctatgcg gataaagccc tgctgaaccg
     4861 aaaaatggat gccctgctgg gtacactggt ggcaatcaat ccggtgcggg cggcagtact
     4921 gtgtgatacg cagcataagg agtgatgacg atgggaagag gttattttct ggtcaggggc
     4981 gataagacca cctgcggcgg caaaattatt gaaggggcgg acgaccatac gatcatggga
     5041 atcccgcagg cgcgggatat ggacagaatg acctgtggca agcatccggg gatgtttatt
     5101 atcgtcggcg gcgtgccgga aacgggtatt cacggcaggc tgatggcggg atcgctggac
     5161 agccagagca gttgcccgtg taaggcgcgg tttattgcgt cgatgatgga tgatgtgtat
     5221 gaggtggaaa cagaaaaaac tgagaacaag tctgttatgc ccttgaaccc tttaaacaat
     5281 ggaacaggaa attcatcacc tcctgcttat acctctgatt atcctgccct tcgtaatacg
     5341 cacaatctgc cagaccagca ggtcagggat atgctagcga ataataatca tgatgttatg
     5401 ttactgacgt atgaagaggg cgctgaagtt ctgcaatcgt ggggttgtca ggattttaag
     5461 cggaaatggg ttgacatcac gcaatctgca ccagggcagt taattattaa ttatggcgta
     5521 aatggtaaag atgtagtgac aacttcaatc gtgcttgccc gcctggggga ttttggtatc
     5581 aaagcaactg cctatgttaa tcataaagga accgaactga taaaacttac aggatatccc
     5641 ggcattcgga aaatactgaa tgcaccggtg tttgcagcta aaaaccccaa aatagtagat
     5701 gttggaattg ggaaatatgg actcacaaac tctatagtaa aaggggcaag attaacgttt
     5761 tacgtcgcag ctgcttatag aactattgat ttcatcctga atgatgaaac atcgttggct
     5821 gagtttatag gtgcgttggc aacagatgtt ggtaaaattg cgatagcttc agcagttagt
     5881 tggggcgcag gaaaggcgtt actggccact gggtttgttg ctggaccttt ggttggggta
     5941 gtggttgtgg ggatcggcgc ggcccttcta ttaaactatc ttgataataa atttggttta
     6001 actgatcaag ttgtcgctta tattgaagcg gctcagcaag aatttgttga gaaggcaaga
     6061 gagattgaag aagggatttg ggatctggga gccatgtggg caaaacacat gcttattaaa
     6121 gggaaggaag
//
LOCUS       DAZGJT010000180         5862 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZGJT010000180 DAZGJT010000000
VERSION     DAZGJT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.184"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(221..619)
                     /locus_tag="ACHKFK_004590"
                     /pseudo
     CDS             complement(221..619)
                     /locus_tag="ACHKFK_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
     gene            754..858
                     /locus_tag="ACHKFK_004591"
                     /pseudo
     CDS             754..858
                     /locus_tag="ACHKFK_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(866..1846)
                     /gene="nanS"
                     /locus_tag="ACHKFK_004592"
     CDS             complement(866..1846)
                     /gene="nanS"
                     /locus_tag="ACHKFK_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9054765.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDKEDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSEPHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVDAFRRALKQYHSQLNKIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNVPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISNRFVEAILQFWRER"
     gene            complement(1911..3017)
                     /gene="nanM"
                     /locus_tag="ACHKFK_004593"
     CDS             complement(1911..3017)
                     /gene="nanM"
                     /locus_tag="ACHKFK_004593"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO9054766.1"
                     /translation="MNKTITALTIIMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTTIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVASPDGVAGGFAGMSNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSADIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            complement(3037..3753)
                     /gene="nanC"
                     /locus_tag="ACHKFK_004594"
     CDS             complement(3037..3753)
                     /gene="nanC"
                     /locus_tag="ACHKFK_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO9054767.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            5208..5810
                     /gene="fimB"
                     /locus_tag="ACHKFK_004595"
     CDS             5208..5810
                     /gene="fimB"
                     /locus_tag="ACHKFK_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO9054768.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEIQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIVSTSGGNAGLSLEIHPHMLRYSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT         1606 a         1267 c         1217 g         1772 t
ORIGIN      
        1 caatccttat tcgtttttac gagtatatta ttcctgtctt cctaccatat tctcatgaaa
       61 ttttttcctc tcagttataa ctttacttta tcttcagcac taagattcaa agattgtaat
      121 actgatcttt cccagcaata atggacacgt ggctaagtgt gtaaactctc agtcagaagt
      181 aactcacatg acatagtatc aaccagatag gtgtattacc tcacccccta aaaaactctt
      241 tccgatgcca ctctacaata ccctcaaaag ggtaattttt acgcacctgt ggcaacgcaa
      301 taatttttcc gtcaaaatcc cagaatgacc tctcaatgat ctctccgccg tttaccgcat
      361 ccgataccag tacccgcaga ttttcatcca ggccaatcgt ttctttatca aaggctttat
      421 gatgtatcgc gcaaagcgcc agaccattgg gaatttcaca agggccatca cgctgtttcc
      481 attttatatg tgtcgcttcc agtgagacgg tggagttgtc gtgacgaata ttaaagctac
      541 atattgcgca ctggtagtta taagcgcgta atacatgttt gcgaaataat gggtcacgct
      601 gcttacggat ttgctggaga gcaaaaaaag ctcgtcagcg atttactgga agccattgat
      661 atcctagcag tgaaataatt gcgatcatgc tcgcacattt tctactgttc aagcctgcaa
      721 gcgaagatat attaccttga actcatttgt tttttgctgc ctgtgtgccc taattgccat
      781 gcgatgttgc accgacgcaa accaccgttt acgccggagg agttaaaagc gctgatggat
      841 gcgaataaat caaattaatg aacgctcacc tttcgcgcca aaactgcaaa attgcttcta
      901 caaacctgtt agaaataatc cctcgacggg ctgccgtgct gaaatgactg cttcgcagtg
      961 ccgtcgtcca gttctccggt gaccggtacg ctgaaccgta atatcccgtg cttaaatcgt
     1021 ccgggtcttc atcaggcacg ttcgtcagtc ctctttcacc ttgttgctgg aagtcgacga
     1081 aaataatatt ggctaaaaca ttattttgat aattgccata aatagcttca tacgcatgag
     1141 ggaaattttc tttccagtac caggtggtat cgccgcaaaa ccacggcgcg tcagtaattt
     1201 tattaagctg agaatggtat tgttttagag ccctacgaaa ggcgtcaacc atatgattaa
     1261 agtgttgagg gtgtgacgcg tagtcactgg tcattaagtc aaattcgcct tgcatccagc
     1321 atacgccgag gaatttgttc tgcggatttt ttgccagtgc ggctcgcgtt ctgctgacta
     1381 aatcctggta tagcggagta tccgatcccc aacgacaagc atcatggctg gctccgtgcg
     1441 gttctgaata tgtcccttcg ctgcccgcgg taaaagctga tccgccacgg caacacggaa
     1501 cgatgagaac ccctgcatta tcaggaataa agggcagtaa tttccgtgct atatgcagtg
     1561 cctggccaac ggtgccgtac tgtgtttgat gattcgttgc cagaggatgg tgataaccct
     1621 gcatatcctg aacatcgtgt gggcagtgag tcagtggaat aatgtcgtta aagtgacatg
     1681 acgggcctcc gggatgtgta tgcgcaaatc tcgctaattg tttaattctg ggatgaggcg
     1741 catcttcctt gtccggtaat ggtagtcctt cgccatacgc catggcatta gactgaccag
     1801 caacggtaag aacatagtaa taatcgggcg atattattgc gttcatataa ttttcgcgta
     1861 atacaaatga caaattgata gcagggcccc tttaccgggg ccctgaagcg ttagttttgt
     1921 actgtgactt tattatcctt cacagaaatc aaaactgaat ccgtcaccgc tttgccgcct
     1981 gcagtttcac cgccaataat taataaacta ttattccagg gcaatgatac tccgtaggcc
     2041 cgaccttgcg ataattcgcc cgatttatcc catttcccgt tatgccaaag atgaatatca
     2101 gcgctatatg attttttcag gccttcatgc gcatagttct taccgttctg gtaattttct
     2161 cgtgaacctt tgaatccggc ccctccggca aatataagtg agtcattgct catgcctgca
     2221 aaaccgccag cgacgccatc tggtgatgcg acgggagcaa gcttattcca ttttaaatta
     2281 ttaccggtga aatcaagttc aaatacggca tccgttcgca atcctggttt ggcttcgcca
     2341 ttaataagcc aggttttatc acctttattc acaaccgccg caccagccgt tccgtaccag
     2401 ggagattcgc cagcgtaact ccattgctgt gtagagggat caaaagacaa cagaaactta
     2461 ttgaagaaat aatcttctgc ttttttgtca aaatagtggg cgttgatttt atctatagtg
     2521 gttgaatctt ttccagcttc gttgagatct tcaaaatagc cattgaagat attctggtta
     2581 acaccgccag taacataagc cttgccgttg tgtacaaaag tcacatggcc cgccatgccc
     2641 atcggcgcgt gcgacatcaa tttaacccag ctattggttt tggggttgta tttgtgtacg
     2701 tcattaaata cctgagtcaa gccctcgctg tttttgccaa tgccgccaaa cacatacaga
     2761 ttgccatcaa taaatgcaga ggttgcttga tctctcggtc cgccagggaa tgcagctaac
     2821 gctgtccatt ttttatcttt ggcctgtgta tccagcttgt accatgccgt acctgcgcta
     2881 cctaaaccaa tgtagacagt gtcgttatca attgctccgg taccactttt aaatggcaca
     2941 ggagtttccg gtaatacaga cgcgtttgcg gcaaatgaag ccattatgat agtaagcgcc
     3001 gttattgttt tattcattgt gactgtctcc tgtctactac agtttaaatg acacaccaat
     3061 gcgataactg ttttccgata aattatctct gccgttgtaa acaccctgac ggtcaaggta
     3121 gtcatattct atgtatggcg taatatcggg cgtcatatgg tattgtagaa caaatgcatt
     3181 ttccgtcgcc catttcttat ggtttgcata gcgataatcg ttctgtttgc tgtatagcgt
     3241 cgtttgccat gcgaaggtga aatcactatt aatatggtaa gtgacatatc catcccaacg
     3301 atgaacgtta tcacgagaca tatcaccgga taagtcttgt tgtcggtaag ctttccagtc
     3361 gtaacgatag cgaatgccaa aattaagatc ttttgtcgcg tcccaggaca gttttacgta
     3421 gggtccgtat cgtgtgccgt tgctgctaaa atgcgttaac attcccgggc gcaccgtcca
     3481 ttgatcatca agtttaatcg cgtaattaac ttccacctga acatcattga gtgcggcatt
     3541 ttccttttta ttatcatgaa tggtattcca ggtattactt tccatacttg cccaccatcc
     3601 attttgccat ccctcactga ctttgagtcg agtctcatag gcgtggcttc cactacgata
     3661 tccaccacgt acgtccagtg tcgcagcctg ggaaattagt ggtgacgaga agcacagtaa
     3721 taatacgcca gaaagtattt tagccttttt catatatttc actcatttgt aggatacaga
     3781 aagcaataca aagcccgcat aaacaattag catttatgtt gtgtaatatt ttttgccagg
     3841 cttatagtgt ctttggcaac gagtatctgt attttatatt ttttgtataa ggtctcctgt
     3901 gaaaattctc tttccacatt attcaaataa acagagaacc agattaaata cctgagtata
     3961 aaatctcttc tgatgtttaa ttgatttgaa tgttcgtaag ctatatcact tactcaatcc
     4021 attttaccca gagtcataaa atagagtgtc tgttgatctc attttcatat tcctttcaga
     4081 ctgaagtgcg ttttcatcac ccgatgagaa agaactgaag tgatgtttgc catagattgc
     4141 gagatgccct gtctcattga ctatcgcggt gcacctttat acctgttata ccagatcaaa
     4201 aatcacgcaa tccatacaac aaaacgagat ttgcaattcg tgtcacaaaa tatgtcgatc
     4261 tttttctaag aggaagatgc catgtgaagc cagacgaaca cttgaggtgg tcttcaaaaa
     4321 ctaaaggatt tcttttaact gtttgtttta taaacaattt atttatggtc taaacaaggg
     4381 atgctttgca agctaactca gtgagcgtgg tgaaaataag tgtttacccg ccatcaggat
     4441 gatcataatt ctcatcatga aatatgtttc ctggtttttg gcttgtaagt ggtcacccct
     4501 gaagtcgatc tggaaaggct tgtggatgtt ggtgttttca ggatgatgtt tcagataggt
     4561 tgtttgccgt atcgcccggc gaatagctgt gattgaggaa ggtttaagtc gcagtgacca
     4621 aagccatatt taccaacgaa tgtagatgaa aaagcgtctc ctgcgttccc ccatatctcc
     4681 aggaaataca cagtctgaaa acaatctcat ggcgtaagct gacgaaccag gcaggaataa
     4741 tcgctaggga cctaagcatt agcatgataa tagccactaa gaaattactg cgttccatga
     4801 aatagccatt ttgtggcaaa tgcagttgac taataatgtc atatgtgaga cggctagttg
     4861 aacaaatatt aaattttgct gaatttttta tgttgatttt acttgttaca gaacatgtca
     4921 catgatatat agataagatt agttgcatta atgatgaggg ttattattcg attcgtatca
     4981 gattgataaa tatataaagg tacatagcat gcaagagcat ggcgtttgta tggcaaagtt
     5041 attataagta actatcgcta ctccatttaa gttcactcag aagaactggt ccacttacgt
     5101 tagttattaa gcaaacgttc gcttttataa acataatcag gattaaaatg ttggattatt
     5161 gctaacccag cacagctagt gcgcgtctgt aattataagg gaaaacgatg aagaataagg
     5221 ctgataacaa aaaaaggaac ttcctgaccc atagtgaaat cgaatcactc cttaaagcag
     5281 caaataccgg gcctcatgct gcacgtaatt attgtctgac attactttgt tttattcatg
     5341 gtttccgggc gagtgaaatt tgccgattga ggatttcgga tattgatctt aaggcaaagt
     5401 gtatatatat ccatcgatta aaaaaaggct tttcaacaac gcacccgcta ttgaacaaag
     5461 aaattcaggc tttaaaaaac tggttgagta tccgtacttc ttatccgcat gctgagagcg
     5521 agtgggtatt tttatcacgt aaggggaatc cactttctcg gcaacagttt taccatattg
     5581 tctcgacttc cggtggtaat gccgggttgt cactggagat tcatccgcac atgttacgct
     5641 attcgtgtgg ttttgctttg gcgaatatgg gaatagatac gcgacttatc caggattatc
     5701 ttggacatcg caatattcgt catactgtct ggtataccgc cagcaatgca gggcgttttt
     5761 atggcatctg ggatagagcc agaggacgac agcgtcacgc tgttttatag agatagttga
     5821 gataccaggg atggtgttaa aaactacatt attcttacga ta
//
LOCUS       DAZGJT010000181         5811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZGJT010000181 DAZGJT010000000
VERSION     DAZGJT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.185"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..375
                     /locus_tag="ACHKFK_004596"
     CDS             <1..375
                     /locus_tag="ACHKFK_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO9054769.1"
                     /translation="YPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLA
                     SYLITHSGNYRYNSTESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNL
                     FKKIAVKNRTQAVSWANDNLRR"
     gene            380..769
                     /gene="csgE"
                     /locus_tag="ACHKFK_004597"
     CDS             380..769
                     /gene="csgE"
                     /locus_tag="ACHKFK_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO9054770.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            794..1210
                     /gene="csgF"
                     /locus_tag="ACHKFK_004598"
     CDS             794..1210
                     /gene="csgF"
                     /locus_tag="ACHKFK_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO9054771.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1237..2070
                     /gene="csgG"
                     /locus_tag="ACHKFK_004599"
     CDS             1237..2070
                     /gene="csgG"
                     /locus_tag="ACHKFK_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO9054772.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2134..2625)
                     /gene="ycdZ"
                     /locus_tag="ACHKFK_004600"
     CDS             complement(2134..2625)
                     /gene="ycdZ"
                     /locus_tag="ACHKFK_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO9054773.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(2727..3281)
                     /gene="ycdY"
                     /locus_tag="ACHKFK_004601"
     CDS             complement(2727..3281)
                     /gene="ycdY"
                     /locus_tag="ACHKFK_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO9054774.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEGATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3305..4042)
                     /gene="ycdX"
                     /locus_tag="ACHKFK_004602"
     CDS             complement(3305..4042)
                     /gene="ycdX"
                     /locus_tag="ACHKFK_004602"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO9054775.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTKAMIATIASGNVHIISHPGNPRYPIDFKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4097..5035)
                     /gene="ghrA"
                     /locus_tag="ACHKFK_004603"
     CDS             complement(4097..5035)
                     /gene="ghrA"
                     /locus_tag="ACHKFK_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO9054776.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLKPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGLQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVDYISRTIAQLEKGERVCGQVDRARGY"
     gene            5269..5356
                     /locus_tag="ACHKFK_004604"
     tRNA            5269..5356
                     /locus_tag="ACHKFK_004604"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5303..5305,aa:Ser,seq:gga)
     gene            complement(5506..5811)
                     /locus_tag="ACHKFK_004605"
                     /pseudo
     CDS             complement(5506..5811)
                     /locus_tag="ACHKFK_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         1549 a         1457 c         1352 g         1453 t
ORIGIN      
        1 tacccgtacc gcgacattga aaactggcct catatcaacg gcgtttttta tgccatggag
       61 gatcaagaac gtgttgtcaa tgggttgcaa ggcgtcctgc gcggcgaatg ctactttacg
      121 caaaagcttg ccagttacct gattacgcat tcaggtaact atcgttataa cagcacggaa
      181 tcagccctcc ttactcatcg ggaaaaagag atcctgaata agctgcgtat cggcgcgtct
      241 aataacgaga tcgctcgttc gttgttcatc agcgaaaata cggttaaaac gcatctttat
      301 aatcttttca agaagatagc cgtaaaaaac cggacacaag cggtttcctg ggcaaacgat
      361 aacctcaggc gataaagcca tgaaacgtta tttacgctgg attgtggcgg cagaatttct
      421 gttcgccgca gggaatcttc acgccgttga ggtagaagtc ccgggattgt taactgacca
      481 tactgtttca tctattggcc atgattttta ccgagccttt agtgataaat gggaaagtga
      541 ctatacgggt aacttaacga ttaatgaaag gcccagtgca cgatggggaa gctggatcac
      601 aataacggtg aatcaggacg ttattttcca gactttttta tttccgttga aaagagactt
      661 cgagaaaact gtcgtctttg cactgattca aactgaagaa gcactaaatc gtcgccagat
      721 aaaccaggcg ttattaagta cgggcgattt ggcgcatgat gaattttaaa taaaaaattg
      781 ttcggaggct gcaatgcgtg tcaaacatgc agtagttcta ctcatgctta tttcgccatt
      841 aagttgggct ggaaccatga ctttccagtt ccgtaatcca aactttggtg gtaacccaaa
      901 taacggtgct tttttattaa atagcgccca ggcacaaaac tcttataaag atccgagcta
      961 taacgatgac tttggtattg aaacgccatc agcgttagat aactttactc aggccatcca
     1021 gtcacaaatt ttaggtgggc tactgtcgaa tattaatacc ggtaaaccgg gccgcatggt
     1081 gaccaacgat tatattgttg atattgctaa ccgcgatggt caattgcagt tgaacgtgac
     1141 agatcgtaaa accggacaaa cctcgaccat ccaggtttcg ggtttacaaa ataactcaac
     1201 cgatttttaa gccccagctt cataaggaaa ataatcatgc agcgcttatt tcttttggtt
     1261 gccgtcatgt tactgagcgg atgcttaacc gccccgccta aagaagccgc cagaccgaca
     1321 ttaatgcctc gtgctcagag ctacaaagat ttgacccatc tgccagcgcc gacgggtaag
     1381 atctttgttt cggtatacaa cattcaggac gaaaccgggc aatttaaacc ctacccggca
     1441 agtaacttct ccactgctgt tccgcaaagc gccacggcaa tgctggtcac ggcattgaaa
     1501 gattctcgct ggtttatacc gctggagcgc cagggcttac aaaatctgct taacgagcgc
     1561 aagattattc gtgcggcaca agaaaacggc acagttgcca ttaataaccg aatcccgctg
     1621 caatctttaa cggcggcaaa tatcatggtt gaaggttcga ttatcggtta tgaaagcaac
     1681 gtcaaatcgg gcggggttgg ggcaagatat tttgggatcg gtgccgacac gcaataccag
     1741 ctcgatcaga ttgccgtgaa cctgcgcgtc gtcaatgtga gtaccggtga gatcctctcc
     1801 tcggtgaaca ccagtaagac gatactttct tatgaagttc aggccggggt tttccgcttt
     1861 attgactacc agcgcttgct ggaaggggaa gtgggttaca cctcgaacga acccgttatg
     1921 ctgtgcctga tgtcggctat cgaaacaggg gtcattttcc tgattaatga tggtattgac
     1981 cgtggtctgt gggatttgca gaacaaagca gaacggcaga acgacattct ggtgaaatac
     2041 cgccatatgt cggttccacc ggaatcctga cagataaaaa agccggaatt atcatccggc
     2101 attttacggt ttaagcgaaa gcgtgagtgc ccctcacgct ttatttttga tttcctgctc
     2161 acggtgcgcc gtctttgcac tacgcgccgc caaccacagg ccgctgtttt tcattgcgta
     2221 accaaatacc agtccaagcg ccagcgaagg taacaccagt ttccagtcac cctgcccggc
     2281 aaacgtcgcg catgcgccta taaacgtccc cggaacaaat gaaagcagca gctgtttggc
     2341 ctggatacac atcagaaacg cgacaatacc ggttatgaca taaccgagta tttccagatg
     2401 tggtgccaat gcactaccgt aaataatgac catcgcccac accacgccac taagcagggt
     2461 tgcagcggag atcgccagcc ctttcagccc accttgcggg caggcaaagt aggccgtaca
     2521 gcccaggaag cccgcccagc ttagtaagcc cagggaaaca gccacccatc cccagatacc
     2581 ggagagaatg cctgttgtga ttgcaataga gagaagtatg ttcatgccgc gcatgatagc
     2641 agaaaacgcg gccatgaatg agatcgagca cacattttat gcaacaacaa attacccgtt
     2701 gcagttatga gtgatgcaga tcacatttac tcttcagaat cttcttccag ctcgtcccac
     2761 attgcactaa tagcatcgcg ggttagcggt gccatggtgc gccagaaagg cgtagttgca
     2821 tgggcctcca ctttgccaag gaacgcacca caccagggta acagatactc actgaacagt
     2881 gtttccagtg cttcgctctc atcttccgtt gactgatctt ccagccagga agccgcaagc
     2941 agcaatgtgc cgatgtgatc ggctggcgta tccgctagtg gcatccctcg ttcggaaaga
     3001 aaagcgcgca cttccgcttc cgttgcgccc tcaacccatg cgctacgata tggcggcaca
     3061 gcacattcat cgccgataaa caacgcattg taatcggcag agacttgcgt catatcacag
     3121 cttttctgta aacgcgtcag taactcatcc tgctccagtg gccagttcgc agccagtttc
     3181 ccctcacgaa tcagggtaaa caacggcacc agtaaaggat cttgcggttg gcggtaatag
     3241 agcgaaccca gcacacgaca gaggatagaa aactcattca ttaatatatt tccattacta
     3301 gtaattaaag gtctgcaaat tccgcaatcg gtgccatacc gcgagattca aggaagttaa
     3361 gtaagcggcg cggagaaaca ttcaaaatgc gctctggcgg aaaatctacc gcgtcgagga
     3421 ttttaagaca ctcttcaaat tcccccatgg taaacgcagt gtgagaatcc gagcctaatg
     3481 ccacccaacc tccagcgtca cgtaccgctg cggctaccgc acgacagttg tcttcactgc
     3541 ccttacgtga gtgtaaaaat gaggaattat tgatttccag cgccacctga tgtttcgctg
     3601 cagcttcagc aacagcttta aaatcaattg gatatcgtgg atttccggga tgacttatta
     3661 tatgaacatt gccgctggcg atagtggcga tcatcgcttt tgtattggtc gctttgtcat
     3721 gtggcgcaaa aaccggctca tgaaaaccgg caataatcag atccagcgaa tcaaacattt
     3781 tgccgctgca gtcaatttca ccatcaacat ttttaatgtt agcttcgatg ccgcgcagga
     3841 tccctacccc atcaaccact cgcggccaga tacgcatgtt aatgaagtgc cagtgatgcg
     3901 gcgcatcttc catatccggg ccatgatcgg tgatcgcaaa aagtttaatg cccttttgtt
     3961 tggcctgggc aatgtaatca ctcaatgtgc tatatgcatg cgtgctggca acggtatgca
     4021 tatgaaggtc gacgggatac ataactctct cctgtattca attcccgcca aggatagcag
     4081 gaaacctgat gctttattag tagccgcgtg cgcggtcgac ttgcccgcag accctctccc
     4141 ctttttcgag ctgggcaatg gtgcgcgaaa tgtaatccac agcttcagcg ggacgggtaa
     4201 tcgcggcgac atgtggtgtt atcgtcacgc gtggatgttg ccagagcgga ctttccggcg
     4261 gtaagggttc acgattaaaa acatccagca ttgcgccttt aactttgccg ctatccagcg
     4321 ccgcgagcag gtcatcttcc acgacatgaa caccacgcgc caggttgagg agatacgcgc
     4381 catccggtaa tttttcgagt aattgttgat taataatgcc gacggtttca ggggtattcg
     4441 gtaacaaatt aatcaatacc cggcattggc tcagaaatgc agaaagttct tcccgtccgg
     4501 caaaactttg caagccaggc cacgatttac gggttcgact ccagcaacgc agcgggaagc
     4561 gccaggtttg cagactctgt gcaactttac tgcccagtac gcctgcgccc aaaatgccga
     4621 tggtaaaatc ttcccgatga tattcaggaa gcggttgcca atgcgaacta ttttgctgga
     4681 tgcgataatc gtcaaaacgt cgaaaccaat gcagcacctg actgacagca tattcctgca
     4741 tttgctcgcc cataccggta tcttccaggc gaaaaagtgg aacagaaggc ttcagcattt
     4801 cagggtgtgc ctgtagcttg ctcaaaatag aatcaacacc ggccccgagt gcgaacaccg
     4861 ctttaagatc gcgccctgcc agcatttcaa ctggaggatg ccagactaaa gcataatcag
     4921 cagagtcatt atctccgctt ttccatgctc tgactcttgc ctgaggaata gctttgcgca
     4981 gtgcctcaat ccaccattgg gtatcgaacg ttgggtgata aaagatgata tccatactga
     5041 ctcccgaaaa gcgttgcgcg aaatttattc gcacttatcg ttatgatcta caaaagccac
     5101 cagcataaca aatccgtggt cggtggcaaa aaaagcaaat ttcgcttatt aaaactgcat
     5161 attgattgaa gtttgaataa tcgagcgatt ttttaaaaaa gtttgttgac ctcaggtcat
     5221 gatttcccta aattagcgcc cgttccagca agacaggaac gacaatttgg tgaggtgtcc
     5281 gagtggctga aggagcacgc ctggaaagtg tgtatacggc aacgtatcgg gggttcgaat
     5341 ccccccctca ccgccagatt acaagcgcat gttattgttt aacatgcgct tttttattta
     5401 tccttccaga cagtatgcca ccgggtatgc catctgaaac cggtttatat gccccacgat
     5461 catatcttat cggacaattt ccgagctttg agcatactgg caaagctata ctgccagcat
     5521 gtcaggatag tgtttttgcg ataatatcat tcagtctgtc aaccagttct ggcttcctga
     5581 acgtgatgcg cgcagagccc ttcttaaagt atttaataat gaacatctca tcttcatagc
     5641 attcttttcc ctgaagagac cggatgtgat cgtccagatg aatagttatg ttttccctgt
     5701 tgtcggggat cggtttgccg ctgaacagat gcagcattct ttccaggtcg gcaagtctat
     5761 ctgcctgttg cccggtgata agatgcaatc cccaccggtc ccacctcatc a
//
LOCUS       DAZGJT010000182         5673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZGJT010000182 DAZGJT010000000
VERSION     DAZGJT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.187"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            154..1488
                     /gene="garP"
                     /locus_tag="ACHKFK_004606"
     CDS             154..1488
                     /gene="garP"
                     /locus_tag="ACHKFK_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO9054777.1"
                     /translation="MMLDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1504..2274
                     /gene="garL"
                     /locus_tag="ACHKFK_004607"
     CDS             1504..2274
                     /gene="garL"
                     /locus_tag="ACHKFK_004607"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO9054778.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2304..3194
                     /gene="garR"
                     /locus_tag="ACHKFK_004608"
     CDS             2304..3194
                     /gene="garR"
                     /locus_tag="ACHKFK_004608"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO9054779.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3291..4436
                     /gene="garK"
                     /locus_tag="ACHKFK_004609"
     CDS             3291..4436
                     /gene="garK"
                     /locus_tag="ACHKFK_004609"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO9054780.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDSGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTNDVGVVH
                     QHGIDAVFSVLTSIGTLDEALRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4469..4845
                     /gene="rnpB"
                     /locus_tag="ACHKFK_004610"
     ncRNA           4469..4845
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKFK_004610"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(4989..5051)
                     /locus_tag="ACHKFK_004611"
                     /pseudo
     CDS             complement(4989..5051)
                     /locus_tag="ACHKFK_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5054..5146)
                     /locus_tag="ACHKFK_004612"
                     /pseudo
     CDS             complement(5054..5146)
                     /locus_tag="ACHKFK_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5403..5621)
                     /gene="tdcR"
                     /locus_tag="ACHKFK_004613"
                     /pseudo
     CDS             complement(5403..5621)
                     /gene="tdcR"
                     /locus_tag="ACHKFK_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
BASE COUNT         1389 a         1305 c         1540 g         1439 t
ORIGIN      
        1 gtattaatta aattagcatt tgcacaatga ttattattct cgcgctgctt atactaattc
       61 ccgagttcat tgtcgtaacg gcataaatga aactcataaa taacaccttc cccataataa
      121 taaaaatcag cataagtacc cgaggtaaat aaaatgatgc tggacaccgt tgacgaaaaa
      181 aagaaaggcg tgcatacccg ctatttaata ttactgatta tttttattgt taccgccgtt
      241 aactacgccg atcgtgcaac gctgtctatt gctggtaccg aagtggcaaa agagttgcag
      301 ttaagtgcgg tttcgatggg ttacatcttc tctgcttttg gctgggccta cttgctgatg
      361 caaattcccg gcggctggct gcttgataag tttggctcga agaaagttta cacttacagc
      421 ctctttttct ggtcgctgtt caccttcctg caaggctttg tcgatatgtt tccgctggcc
      481 tgggctggga tctccatgtt ctttatgcga tttatgctgg ggttctcgga agcgccatca
      541 ttcccggcga acgcccgtat tgtcgccgcc tggttcccga cgaaagaacg tggtactgcc
      601 tccgctatct ttaactcggc gcaatatttc tcgttggcgc tcttttcgcc gctgcttggc
      661 tggctgactt tcgcctgggg ctgggagcac gtctttaccg ttatgggggt gattggtttt
      721 gtgctgacgg cgctgtggat caagttgatt cataatccga cagatcaccc acgtatgtct
      781 gcggaagagc tgaagtttat ctcggaaaat ggcgcggtgg tcgatatgga ccacaaaaag
      841 ccgggcagtg cggcagcaag cggacccaaa ctgcattaca tcaagcaatt gctctctaac
      901 cgcatgatgc tgggcgtatt tttcggacaa tattttatca acaccatcac ctggttcttc
      961 ctcacctggt tcccaattta tctggtgcag gaaaaaggca tgtcgattct gaaagtgggt
     1021 ctggtcgcct cgattccggc actgtgtggc tttgcgggcg gcgtgctggg aggtgtcttc
     1081 tcggattatc tgatcaaacg cggtttgtcc ctgaccctgg ctcgtaagct accaattgtg
     1141 ctgggaatgt tgctggcttc caccatcatc ttatgtaact acaccaacaa caccacgctg
     1201 gtggtcatgc tgatggcgct ggctttcttt ggtaaaggat ttggtgcgct gggctggcca
     1261 gtgatttctg acaccgcgcc gaaagagatt gttggcctct gcggcggcgt ctttaacgtc
     1321 tttggcaatg ttgcctccat tgttactcca ctggtgattg gctacctggt aagtgaactg
     1381 cactccttca atgcagcact ggtttttgtg ggatgttcag cgctgatggc gatggtctgc
     1441 tacctcttcg tagtcggcga cattaaacgt atggaattgc agaaataagc aaaggtataa
     1501 gcgatgaata acgatgtttt cccgaataaa ttcaaagccg cactggctgc gaaacaggta
     1561 caaattggtt gctggtcagc tctctctaac ccgattagca ctgaagttct tggtttggct
     1621 gggtttgact ggctggtgct ggatggtgaa catgcgccaa acgatatctc cacgtttatt
     1681 ccgcagttaa tggccctgaa aggcagcgcc agcgcgccag tagtgcgagt gccgaccaac
     1741 gagccggtaa ttattaagcg tcttctggat atcggtttct ataacttcct gattcctttt
     1801 gtagaaacaa aagaggaagc agagcaggcg gtggcatcaa ctcgttaccc accggaaggc
     1861 attcgcggcg tctccgtttc tcaccgcgcc aatatgtttg gcaccgtggc ggattatttc
     1921 gctcagtcga acaagaacat cactattctg gtccagatag aaagtcagca gggcgtagat
     1981 aacgtcgatg ctattgccgc taccgaaggt gtagacggca tcttcgtcgg ccccagcgat
     2041 ctggctgcgg cattaggcca tctcggcaat gcctcgcacc cggatgtaca aaaagcaatt
     2101 cagcacattt ttaaccgtgc cagcgcgcac ggcaaaccca gcggcatcct cgcaccagtc
     2161 gaggctgatg cgcgtcgtta tctggaatgg ggcgcgacgt ttgtggccgt cggcagcgat
     2221 ctcggcgtct tccgctctgc cactcagaaa ctggctgata cctttaaaaa ataaccacca
     2281 ccgcaataag agagatgatt gatatgacta tgaaagttgg ttttattggc cttgggatta
     2341 tgggtaaacc aatgagtaaa aaccttttga aagcaggtta ctcgctggtg gttgctgacc
     2401 gtaacccaga agctatcgct gacgtgattg ctgcaggtgc agaaacagcc tctacggcta
     2461 aagcgatcgc tgaacagtgc gacgtaatca taaccatgct gccaaactcc cctcatgtga
     2521 aagaggtggc gctgggtgag aatggcatta ttgaaggcgc gaagccaggt acggtattga
     2581 tcgatatgag ttctatcgca ccgctggcaa gccgtgaaat cagcgaagcg ctgaaagcga
     2641 aaggcattga tatgctggat gctccggtga gcggcggtga accgaaagcc atcgacggta
     2701 cgctgtcagt gatggtgggc ggcgacaagg ctattttcga caaatactat gatttgatga
     2761 aagcgatggc gggttccgtg gtgcataccg gggaaatcgg tgcaggtaac gtcaccaaac
     2821 tggcaaatca ggtcattgtg gcgctgaata ttgctgcgat gtcagaagcg ttaacgctgg
     2881 caaccaaagc gggcgttaac ccggatctgg tgtatcaggc aattcgcggt gggctggcgg
     2941 gcagtaccgt gctggatgcc aaagcgccga tggtgatgga ccgcaacttc aaaccgggct
     3001 tccgtattga tctgcatatt aaggatctgg cgaatgcgct ggatacttct cacggcgtcg
     3061 gcgcgcaact gccgctcaca gctgcggtta tggagatgat gcaggcactg cgtgcagatg
     3121 gtttaggaac ggcggatcat agcgccctgg cgtgctacta cgaaaaactg gcgaaagtcg
     3181 aagttactcg ctaatgacgt cgtgcccgga tggcgtattg caatccgggc ctggaatcca
     3241 ggccgaataa gagaaacgcc ctccggcgtc atgtggtaac aggcataggt atgaaaatcg
     3301 taatcgcccc agactcttat aaagaaagtt tatctgccag cgaggttgcg caggcgatag
     3361 aaaaaggatt tcgggaaatt tttcctgatg cacagtatgt ttctgttccg gttgccgacg
     3421 gtggtgaagg aacggtggaa gcgatgattg cagccaccca gggggctgaa cgtcacgcct
     3481 gggttacagg gccgctgggc gagaaagtga atgccagttg ggggatctcc ggcgatggca
     3541 aaaccgcgtt tatcgaaatg gcggcggcca gtgggctgga actggtacct gcggaaaaac
     3601 gcgatccact cgtgaccact tcacgcggta ctggtgagtt aatcctgcag gcgctggaga
     3661 gcggtgcgac aaacattatt atcggcattg gcggcagcgc caccaacgac ggcggcgcag
     3721 gcatggtaca ggcgctgggg gcgaaattat gcgatgccaa cggcaatgaa attggttttg
     3781 gcggcggtag tcttaatact ctgaatgata ttgatatttc cggtctcgat ccgcgcttaa
     3841 aagattgtgt catccgcgtc gcttgtgatg tcaccaaccc gttagtcggc gacagtggtg
     3901 cttcgcgcat ctttggccca caaaagggag ccagtgaggc gatgattgtc gagctggaca
     3961 ataacctctc tcactatgcc gatgttatta aaaaagcgct gcatgttgat gtgaaagatg
     4021 tccccggcgc aggagctgcg ggaggtatgg gcgcggcgct gatggcgttt cttggtgcag
     4081 aactgaaaag tggtattgaa atcgtcacga cggcgctgaa tctggaggaa catattcacg
     4141 attgtacgct ggtgatcacc ggtgaagggc gtattgacag ccagagtata cacggcaagg
     4201 ttccgattgg cgtcgcaaac gtggcgaaga agtaccataa accggtgatt ggcattgcgg
     4261 gtagcctgac caatgatgtt ggcgttgtac atcagcatgg cattgatgcg gtcttcagcg
     4321 tactgaccag cattggtacg ttagacgaag cactccgcgg agcttatgac aatatctgcc
     4381 gtgcttcacg taatatcgcc gcgacactgg cgattggaat gcgcaacgcg gggtgacaag
     4441 ggcgcgcaaa ccctctatac tgcgcgccga agctgaccag acagtcgccg cttcgtcgtc
     4501 gtcctcttcg ggggagacgg gcggagggga ggaaagtccg ggctccatag ggcagggtgc
     4561 caggtaacgc ctggggggga aacccacgac cagtgcaaca gagagcaaac cgccgatggc
     4621 ccgcgtaagc gggatcaggt aagggtgaaa gggtgcggta agagcgcacc gcgcggctgg
     4681 taacagtccg tggcacggta aactccaccc ggagcaaggc caaatagggg ttcataaggt
     4741 acggcccgta ctgaacccgg gtaggctgct tgagccagtg agcgattgct ggcctagatg
     4801 aatgactgtc cacgacagaa cccggcttat cggtcagttt cacctgattt acgtaaaaac
     4861 ccgcttcggc gggtttttgc ttttggtgcc cagggtaaga tgaatatcta cggtattaat
     4921 aacagacttt gtaagaagtt attaagtcta tattgcattt aaaaggtcgt aattatacat
     4981 atatttctct cattaattgt aagttcgcag gtattgtccg atcgttgaat catttgggcg
     5041 ttataaccta aatttttacc gtccaataat caatatttct attgtgattg ttaaccactg
     5101 cgtgcattga agggtgaaaa atatgtgcaa ttgagaagcc tttcattgat gccagccttg
     5161 tcatcatgac aaacaataat agatgcttat tttcctcgca gggttctttc gcactatcaa
     5221 aaaatgctca tttacccggc attacagtca acgaagcaac attctatggc ctacgctgat
     5281 gtaaagcaca gcgaaaatcc tccttgttga cttcgtctga ccttatcgcg gcgaccagca
     5341 attattcgtt ttgtgatcct caacacaaaa tgctattcgt tgcctatgag ggggagaaga
     5401 atttatggaa aacgattgca ttcacaaaat gacgtcctgc cagaagagaa aaggttgatg
     5461 ttaatcaatt gttcgagatg atcaatacct gtattctaaa ttttaaaata ttgataaaat
     5521 aaatgtttgt tctccaacaa atagcgtgaa aaaatgtgac gtgccgcatt atttatcgct
     5581 gaaaatggtt tgtttataat aaaatttgag aacttactca ttaaatttaa ctcaaatttt
     5641 gcctggtaat tatccggtaa ttgcttgaaa tat
//
LOCUS       DAZGJT010000183         5644 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZGJT010000183 DAZGJT010000000
VERSION     DAZGJT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.188"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            381..2177
                     /locus_tag="ACHKFK_004614"
     CDS             381..2177
                     /locus_tag="ACHKFK_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aryl-sulfate sulfotransferase"
                     /protein_id="HFO9054781.1"
                     /translation="MFDKYRKTLVAGTVAITLGLSASGVMAAGFKPAPPAGQLGAVIV
                     DPYGNAPLTALVDLDSHVISDVKVTVHGKGEKGVEISYPVGQESLKTYDGVPIFGLYQ
                     KFANKVTVEWKENGKAMKDDYVVQTSAIVNNYMDNRSISDLQQTKVVKVAPGFEDRLY
                     LVNTHTFTAQGSDLHWHGEKDKNAGILDAGPATGALPFDIAPFTFIVDTEGEYRWWLD
                     QDTFYDGRDRDINKRGYLMGIRETPRGTFTAVQGQHWYEFDMMGQVLEDHKLPRGFAD
                     ATHESIETPNGTVLLRVGKSNYRRDDGVHVTTIRDHILEVDKSGRVVDVWDLTKILDP
                     KRDALLGALDAGAVCVNVDLAHAGQQAKLEPDTPFGDALGVGPGRNWAHVNSIAYDAK
                     DDSIILSSRHQGVVKIGRDKQVKWILAPSKGWEKPLASKLLKPVDANGKPITCNENGL
                     CENSDFDFTYTQHTAWISSKGTLTIFDNGDGRHLEQPALPTMKYSRFVEYKIDEKKGT
                     VQQVWEYGKERGYDFYSPITSIIEYQADRNTMFGFGGSIHLFDVGQPTVGKLNEIDYK
                     TKEVKVEIDVLSDKPNQTHYRALLVRPQQMFK"
     gene            2195..2863
                     /locus_tag="ACHKFK_004615"
     CDS             2195..2863
                     /locus_tag="ACHKFK_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFO9054782.1"
                     /translation="MSKLGISSLFKTILLTAALAVSFTASAFTEGTDYMVLEKPIPNA
                     DKTLIKVFSYACPFCYKYDKAVTGPVSEKVKDIVAFTPFHLETKGEYGKQASEVFAVL
                     INKDKAAGISLFDANSQFKKAKFAYYAAYHDKKERWSDSKDPAAFIKTGLDAAGMSQA
                     DFEAALKEPAVQETLEKWKASYDVAKIQGVPAYVVNGKYLIYTKSIKSIDAMADLIRE
                     LASK"
     gene            2878..3549
                     /gene="dsbI"
                     /locus_tag="ACHKFK_004616"
                     /pseudo
     CDS             2878..3549
                     /gene="dsbI"
                     /locus_tag="ACHKFK_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511507.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide oxidoreductase DsbI"
     gene            complement(3595..4383)
                     /gene="ygiD"
                     /locus_tag="ACHKFK_004617"
     CDS             complement(3595..4383)
                     /gene="ygiD"
                     /locus_tag="ACHKFK_004617"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO9054783.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQTLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMEMPPTIHDFGGFPQALYDTYYPAPGSPALAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            4526..5299
                     /gene="zupT"
                     /locus_tag="ACHKFK_004618"
     CDS             4526..5299
                     /gene="zupT"
                     /locus_tag="ACHKFK_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO9054784.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTVGIG"
     gene            complement(5361..5531)
                     /locus_tag="ACHKFK_004619"
     CDS             complement(5361..5531)
                     /locus_tag="ACHKFK_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054785.1"
                     /translation="MFIAWYWIVLIVLLAGGYFLHLKRYCKAFRHDRDALLAARSKLD
                     KRSDKEVFEKHQ"
BASE COUNT         1515 a         1352 c         1315 g         1462 t
ORIGIN      
        1 taatactcaa tttaatgtta aatcgtcata tgcaagcgat attgtaatta aagtaagtga
       61 atacatcaca acataactat ttgatattat ttaaatactt tacttttatc tcaccagatt
      121 taaatttaat aattaaaaaa caatgttgca gggatcacac tacacagtat tgaacattgt
      181 gattgatata ttgcccccct actatccatt aaacataaat atttgtttca ccgaataaca
      241 acatatttaa aagaaacaca aaagcattct cactctttat tgaattaata tttatattca
      301 cgtaacgaat ttattagcac cctctcaaca gtcgatattc atcattatta tatatttgcg
      361 ttatataagg agatatatcc atgtttgata aatatagaaa aacactcgta gccggaactg
      421 tggcgataac cctgggtttg tcagcatcgg gggtgatggc tgcgggtttt aaaccagcgc
      481 cacctgccgg gcaactgggt gcggtcattg tcgatcccta cggcaatgca ccactgaccg
      541 ctttggttga cttagatagc catgttattt ctgacgtcaa agttaccgtc catgggaagg
      601 gcgaaaaagg cgtagaaatc agctatcccg tgggtcagga atcactaaaa acttacgatg
      661 gtgtaccgat ttttggtctt tatcagaaat ttgctaacaa agtgaccgtt gagtggaaag
      721 aaaacggcaa ggccatgaaa gatgattacg tggtgcagac ttccgccatc gtcaacaatt
      781 acatggataa ccgctccatc tccgacctac agcaaaccaa agtcgttaaa gtcgcgccag
      841 gttttgaaga tcgcctctat ctggttaata cccacacctt taccgcccaa ggttccgatc
      901 tccactggca tggtgagaaa gataaaaatg ccggtatcct tgatgcgggt ccggcaactg
      961 gcgcactccc ttttgatatc gcgccattca cctttatcgt cgataccgaa ggtgaatacc
     1021 gctggtggct ggatcaagac actttctacg atggtcgtga ccgcgatatc aacaagcgtg
     1081 gttatctgat gggtatccgc gaaacgccac gcggcacctt taccgctgta caaggtcagc
     1141 actggtacga gttcgacatg atgggacagg tgctcgaaga tcacaaacta ccgcgcggat
     1201 ttgctgacgc cactcatgag tccattgaga cgccaaatgg cacggtactg ttgcgcgtag
     1261 gtaagagtaa ctatcgtcgc gatgacggcg tacacgtcac caccattcgt gaccatatcc
     1321 tcgaagtcga taaatctggt cgcgttgtcg atgtatggga tctgaccaag atcctcgatc
     1381 cgaaacgcga tgcactgctc ggcgcgctgg atgcaggtgc agtttgcgtt aacgttgacc
     1441 ttgcccatgc aggacaacag gcaaaactgg aaccagatac accgttcggc gacgcgctgg
     1501 gtgtagggcc aggccgcaac tgggcgcacg tgaactctat cgcctatgac gcaaaagatg
     1561 actctatcat cctctcctct cgccaccagg gtgttgtgaa gattggtcgt gataagcaag
     1621 tgaaatggat ccttgcgccc tctaaaggtt gggaaaaacc gctggccagc aagctgctga
     1681 aaccggtgga cgctaacggt aaacctatta cctgtaacga aaacggtctg tgtgaaaact
     1741 cggacttcga ttttacctac acccagcata ccgcctggat ttccagcaaa ggaacgctca
     1801 ccatttttga taatggcgat ggtcgtcatc tggaacaacc tgccttacca accatgaaat
     1861 attcccgctt tgtggaatat aagattgatg agaagaaagg caccgttcag caagtgtggg
     1921 aatacggtaa agaacgtggc tacgatttct atagcccgat tacctccatc attgaatatc
     1981 aagccgaccg taacaccatg tttggcttcg gtggttctat tcatttgttc gatgtcgggc
     2041 agccaaccgt cggtaagttg aacgaaatcg attacaaaac caaagaagtg aaagtggaaa
     2101 tcgacgtgct gtcagataaa cccaatcaga ctcactaccg tgcgctgtta gtccgtccac
     2161 aacagatgtt caaataatta agaagggaat aaaaatgtcg aaattaggga tctcatcact
     2221 atttaaaacc atactgctta ccgcagcttt ggctgtttca tttaccgctt ctgcatttac
     2281 cgaaggcacg gattacatgg ttctggaaaa acctattccg aatgccgaca aaacgctgat
     2341 taaggtattt agctacgcct gcccgttctg ttacaagtac gacaaagcgg ttaccggtcc
     2401 ggtatcggaa aaagtgaaag atatcgttgc tttcacgccg ttccatctgg aaacaaaagg
     2461 tgaatacggc aaacaagcaa gcgaagtctt tgcggtcttg atcaataaag ataaagcagc
     2521 aggcatttcc ttatttgatg ctaactctca gtttaagaaa gccaagtttg cctactacgc
     2581 ggcttatcac gacaaaaaag aacgctggtc tgacagtaaa gatccggctg cttttatcaa
     2641 aaccggtctg gatgccgcag gtatgagtca ggcggatttt gaagcggcac tgaaagaacc
     2701 agcggttcaa gaaacactgg aaaaatggaa agcctcttat gatgtcgcca aaatccaggg
     2761 ggttccagcc tatgtcgtta atggtaagta cttgatctac accaagagca tcaaatccat
     2821 cgacgcgatg gcagacctta tccgtgagtt agcaagcaaa taacagggaa acaggctatg
     2881 ggtattaagg gaatgtggaa agatcttcgc acatcaccag ttgacacact ggtcagatgg
     2941 caggagcaac gcttgttgtg gttgttaatg gccgttgcga tgggcgcgtt aattatcctg
     3001 gcgcactctt tcttccagat ctatctctac atggcaccct gtgagcaatg tgtatacatt
     3061 cgctacgcca tgtttgtgat ggttattgga ggattggttg cagcgatcaa cccaaagaac
     3121 atcatcctaa agctaattgg ctgcgtgatg gcattttacg gcagcatttt gggactgaag
     3181 ttttcgctca aactgaacga tatccaccat gcagtacata atccggatcc cgattaactg
     3241 tttggcgtac agggttgctc taccgatcca acttttccct ttaacctgcc gctggcacag
     3301 tgggcaccaa attggttcaa gcctaccggg gattgtggct atgacgcacc aatcgttcct
     3361 gatggcgtca cactgagcag cacgcaacag tggtttgtgg aaatgtatca gcaatctgaa
     3421 ggctggtatc tgctaccacc atggcatttt atgagtatgg ctcaggcttg tatgctggct
     3481 tttggtatgt gtctggtgct gctggtcatt atgagtgacg catgggcgct taaaatcatc
     3541 agaggctaac tcagcaacac ccgaagccag tcattctggc ttcgggttct tttattaacc
     3601 tatctgtacc gacagcatac ttaaactgcc catttctata ccatcgaccg gaatggtaat
     3661 tggctcctgc ccatcccacg cccctaagac atacaacaac ggcagatagt gctctggcgt
     3721 tgggttcgat aacgcgccac cttcatggtc gagataattc accagaggat gttgttccac
     3781 cggcccttgc cacgtcagat tcgctttcac atactcatta aacgacgtcg cccacggata
     3841 cggtgaacta tcaccgtgcc acttcactgt acgcaggtta tgtaccacgt tgccactggc
     3901 gaccaacatt atcccttcat cacgcagcgc cgcgagtttg cgccccattt cgaaatgcca
     3961 ggcggcaggt ttgctactgt cgatactcaa ctgcaccatc gggatatcgg cgtcagggta
     4021 catcttaatc agtacgcccc acgagccgtg gtcgaagccc caggcttctt tatccagcgt
     4081 cacagggacc ggcgctaaca gctcaaccag atgctgtgcc agcgcaggtg aacccggagc
     4141 aggataatac gtatcgtaca gcgcctgcgg gaagccacca aagtcatgaa tcgtgggcgg
     4201 catctccatc gcggtcactc ctgttccacg ggtaaaccaa tgagccgaaa ccaccactat
     4261 tgcttgcggg cgtggcaatg tcatccccaa cgtctgccag ctgcgggtat acaaattatc
     4321 ttccagcacg ttcatcggac taccgtgacc taaaaacaat gctggcatac gtgttgaaga
     4381 catgatgata tccttaacta aaggtgtcat tttgatatcc tcacaatacg cttgttcggc
     4441 ggagtaagaa cccggataac aatgatgatg atcatcagtt attttgacga tctgcctgaa
     4501 ggtaaagatt tataaggagt tgtcgatgtc agtacctctc attctgacca tactggcggg
     4561 ggcagccacg tttattggcg cgtttctcgg cgttctcggg caaaaaccct cgaaccgctt
     4621 actggcgttt tcgctaggat ttgcggcggg gatcatgttg ctcatctcat taatggaaat
     4681 gcttcctgcc gcattagccg ccgaaggaat gtcgcctgtg ttgggttatg gaatgtttat
     4741 cttcggtctg cttggctatt ttggcctgga ccgcatgttg ccacatgcgc atccgcagga
     4801 tttaatgcaa aaatcggtgc agccgttgcc aaaatcgatc aagcgcacag ccattctgct
     4861 cactctcggc atcagtctgc ataacttccc ggaagggatt gccacctttg tcacggcgag
     4921 cagcaacctg gagctgggat ttggcatcgc cctggccgta gcgttgcaca atattcctga
     4981 aggtctggcg gttgcaggcc cggtttacgc tgcaacagga tcgaaacgta ccgcgattct
     5041 gtgggcgggc atttccgggc tggcagaaat tcttggtggt gtgctggcat ggttaatcct
     5101 cggtagcatg atttctccgg tggtcatggc ggcaatcatg gcggcggttg caggaattat
     5161 ggtggcgctc tccgttgacg aattaatgcc gctcgccaaa gagattgacc ccaataataa
     5221 ccccagctat ggcgtactat gtggaatgtc agtgatggga ttcagtttag tgctgctaca
     5281 aacggtggga attggttaaa gacgataatg acagatgttg cccattgcta aaggcaacat
     5341 ctgtatcgtt attaaaggta ttattgatgc ttttcaaaca cttctttatc ggagcgttta
     5401 tctaatttac ttcgggcagc taacagcgca tctctgtcat ggcgaaaagc tttacagtaa
     5461 cgtttaagat gaagaaaata gccacccgcc agtagaacaa ttaatacaat ccagtaccag
     5521 gcaataaaca tccttttcac cttcaaaatt taacattact aaacatttag cgttaaaatt
     5581 tcacatatcc atcgttgaat acattcatat actcgcataa ttctggatca agtatatgat
     5641 ttcg
//
LOCUS       DAZGJT010000184         5444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZGJT010000184 DAZGJT010000000
VERSION     DAZGJT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.189"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..592)
                     /locus_tag="ACHKFK_004620"
     CDS             complement(56..592)
                     /locus_tag="ACHKFK_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HFO9054786.1"
                     /translation="MLHTIHFLCPVNTATVGQLQNHCLTALSQGATELNIHISSQGGE
                     TAAGFTAYNFLKSLPVTVRTHNISNVESIANIVFLAGSERFANPLSRFLLHPLLWCFA
                     SPAADHARLREYGKCLDNDLDRFVETFNIDIGTHIRWASLIADSTILDANKALEHGII
                     NSIKTARLASNQANWWVV"
     gene            complement(592..840)
                     /locus_tag="ACHKFK_004621"
     CDS             complement(592..840)
                     /locus_tag="ACHKFK_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9054787.1"
                     /translation="MAKGVSISPTTVRIPESLREALAVRASKNGRSVNSEIVMILQAA
                     IDEDRSPKSVESFAQQEADKFKEALLETLKTMYGKDEK"
     gene            955..1107
                     /locus_tag="ACHKFK_004622"
     CDS             955..1107
                     /locus_tag="ACHKFK_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFO9054788.1"
                     /translation="MEKEISKILVRMPQSLKDAIGSRAKEECRSFNSEVIKRLIDSLK
                     REGVMV"
     gene            1370..2272
                     /locus_tag="ACHKFK_004623"
     CDS             1370..2272
                     /locus_tag="ACHKFK_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001084296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFO9054789.1"
                     /translation="MNSIAILEAVNTSYVPFNGQQIITAMAAGVAYVAMKPIVENLGM
                     SWGTQQQKLMKQLDKFNCIHMNMVAADGKLRKLLCLPLKKLNGWLFSINPEKVRADIR
                     DKLIQYQEECFTVLHDYWTKGKAENARKKTSVDDRTPLRDAVNMLVSKKHLMYPEAYA
                     MIHQRFNVESIEELDASQIPQAVEYIHRVVLEGEFIGKQEKKTNELSAKEANSLVWLW
                     DYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYTIGRARGVLINHTRDIDIYEP
                     DGPTNLLAWERLKNKELPPSLHHY"
     gene            2327..3064
                     /locus_tag="ACHKFK_004624"
     CDS             2327..3064
                     /locus_tag="ACHKFK_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000967278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9054790.1"
                     /translation="MLYSNILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGDIHKFR
                     CVPHLAGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHRGDDWWRNGQNLYLDNMEGTGF
                     YRVPLSSAQAGDILLCCFGASVPNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTH
                     SVWRHRHWHASAFTGIYNDLAAASACM"
     gene            3061..3642
                     /locus_tag="ACHKFK_004625"
     CDS             3061..3642
                     /locus_tag="ACHKFK_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9054791.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALSAGGFS
                     ATTMLFSLGASMILGGAAQMLAPKAKTPDYRVTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            complement(3883..4410)
                     /gene="sodC"
                     /locus_tag="ACHKFK_004626"
     CDS             complement(3883..4410)
                     /gene="sodC"
                     /locus_tag="ACHKFK_004626"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO9054792.1"
                     /translation="MNRKSLAAIAMVLATSCGYAATIKVPMNLVNADGKEVSAGTITI
                     QETSYGLLFTPDLHSLPQGIHGFHVHEKGSCAPALKDGVSVAALAAGGHFDPKQTGKH
                     LGPWSPDGHLGDLPALYVTHDGKANYPVLAPKLKELTEISGRSLMIHIGGDNYHDHPD
                     PLGGGGARMGCGIIP"
     gene            4546..>5444
                     /locus_tag="ACHKFK_004627"
     CDS             4546..>5444
                     /locus_tag="ACHKFK_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9054793.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETVLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLALVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWA"
BASE COUNT         1408 a         1229 c         1506 g         1301 t
ORIGIN      
        1 ggattatgcc caccgtcagg tgggtttttt ataaggagta atcatgattt acccatcaaa
       61 caacccacca gtttgcctga ttggatgcca gccttgcagt ttttatggaa ttaattatgc
      121 catgctcaag agccttgtta gcatccaaaa tggtcgagtc tgctatcagg gatgcccacc
      181 taatatgggt tccgatgtcg atattgaacg tctcaacgaa gcgatcaaga tcgttatcga
      241 ggcatttccc gtactctctc aatctggcat ggtcggcggc tggggaggca aagcaccata
      301 atagaggatg taacaggaat cttgataatg ggtttgcgaa acgttctgag ccagccagga
      361 aaacgatatt agctatggat tcaacattgc ttatgttgtg agttctaacg gtaacaggga
      421 gtgacttaag aaagttatac gcagtaaagc cagcggcagt ttcccctccc tgacttgata
      481 tatggatatt taattcagtt gcgccttgag ataatgcggt gagacagtgg ttctgaagtt
      541 gcccaacagt ggcagtgtta acggggcata agaaatgaat tgtgtgcagc attatttttc
      601 atccttacca tacatggtct ttagcgtctc aagcagcgcc tctttgaatt tgtcagcttc
      661 ttgctgagca aatgactcaa ctgactttgg cgacctatct tcatcaatcg cggcttgcaa
      721 aatcatgacg atctcggagt taacagagcg accatttttt gatgctctga cagcaagagc
      781 ctcgcgtaaa gattcaggga ttcttaccgt agttggagaa attgacacac cttttgccat
      841 atcacacctt tggtattcaa tttgatagca aagtgtatgc aaaaaaattt tgactagata
      901 tactcacttt gctatctttt gtattctaaa agagttgttt gtatggaggg taacatggag
      961 aaagaaataa gtaagatttt ggtaaggatg ccgcagtcgt taaaggatgc tataggtagc
     1021 agggcaaagg aagagtgcag gtcgtttaac tcagaggtta tcaagcgcct gatagacagc
     1081 ttgaagagag agggggtaat ggtatgagta aagaatgttg tttctgcggc attagcgaat
     1141 cagacgctga tcaaacatac atttactcta aagaaacagg tcggatgctg tgtagtgact
     1201 gcgtgttgga catcataaga tacaagcatc ttggatgttc tgtcagcatt agcaatatag
     1261 gtgaagtata tgaagggaaa gatataactg atagagcaga aagttgaagc cccaactgcg
     1321 ggaacagtca gggcttcggt tgtcagtaaa tccgtggaga aaaaccaaca tgaatagtat
     1381 agcaatttta gaagcagtga acacctctta cgtaccattc aacggtcagc aaattatcac
     1441 cgccatggct gccggagttg catatgttgc gatgaagcca atcgttgaaa acctcggaat
     1501 gagctggggt actcagcaac aaaaacttat gaaacaacta gataagttca actgtattca
     1561 tatgaatatg gttgctgctg atggaaagct tcgtaagcta ctctgccttc ctttgaagaa
     1621 gttaaatgga tggctgttca gcatcaaccc cgagaaagtt cgtgctgaca tccgcgataa
     1681 actgattcag taccaggaag aatgctttac tgtgctgcat gactactgga caaagggaaa
     1741 ggcagaaaat gcacgtaaga aaacatctgt tgatgacagg actccgcttc gtgatgctgt
     1801 aaatatgctg gtcagcaaaa agcatctaat gtacccagaa gcttatgcaa tgattcatca
     1861 gcgtttcaat gtggaaagta ttgaagagct tgatgcatct cagataccgc aagccgtaga
     1921 gtacatccac agggtagtgc ttgaaggtga gttcattggc aaacaagaga agaaaaccaa
     1981 cgagctttcc gcaaaagaag caaacagcct tgtatggcta tgggattatg ccaaccgctc
     2041 acaggcattg ttccgtgagt tgtatcccgc attaaaactg attcagtctg gctattccgg
     2101 catatgccac gactacggct atgagttctc gtataccatc gggagggcga ggggcgtttt
     2161 aattaatcac acgcgggata tagatattta tgagcctgac gggccgacga accttctggc
     2221 atgggaaagg cttaagaaca aagagttgcc gccttcactg catcactact aacagattac
     2281 caacgaaatg acccagcctc ggctgggttt tttatcagga gttctcatgc tctatagcaa
     2341 tatattggca catgcccggc ggtgtgcgcc tgcggagtcg tgcggcttcg tggtgagaac
     2401 gccggagggg gagcggtata tcccttgtgt gaatatttcc gcagagccgg aggcgtattt
     2461 tcgtattgca ccggaagact ggctgcgggc agagatgcag ggggagattg tggcactggt
     2521 ccacagtcat cccggtggtc tgccctggct gagcgaggct gaccggcggc tgcagataaa
     2581 aagcgcactg ccctggtggc tggtctgccg gggtgacatt cacaaattcc gctgtgtgcc
     2641 acacctggcg ggacggcgct ttgagcacgg ggtgacggac tgttacacgc tgttccggga
     2701 tgcttatcat ctggcgggga ctgaaatgcc ggattttcat cgcggggatg actggtggcg
     2761 caacggccag aacctgtacc tggacaatat ggagggaacg ggcttttacc gggtgcccct
     2821 gtcctctgca caggcgggcg atattctgct gtgctgcttt ggtgcttcgg taccgaacca
     2881 tgccgccatt tactgcggca acggtgagct gcttcaccat ctgcctgaac aactgagtaa
     2941 acgggagagg tattccgaaa aatggcaacg acgaacgcat tctgtctggc gtcaccgcca
     3001 ctggcacgca tctgccttca cggggattta caacgatttg gccgccgcct cagcctgtat
     3061 gtgaacacgg cagcggaagc catccgcgcc ctgtcgatgc agatgccggg ctttcgccgt
     3121 cagatgaacg aaggctggta ccagatacgt attgccggtg atgacacggc accggaggcg
     3181 gtgtacgccc gtcttcacga acagctgggt gagggaacgg tcatccacat tgtgccgcga
     3241 ctggccgggg ccggaaaggg tggactgcag attgtgctgg gggcggcagc catcgtgggc
     3301 tctttcttca cggccggtgc ctcgatggcg ttatggggtt cagccctgag tgccggtggt
     3361 ttttctgcca ccacgatgct gttttcactg ggggccagca tgatactggg cggtgcggca
     3421 cagatgctgg ccccgaaggc aaaaacaccg gattaccgcg taacggataa cggcagacag
     3481 aacacgtact tttcctcgct ggataacatg attgcccagg ggaacccgat gccggtgcct
     3541 tacggggaaa tgctggttgg ctcccggcga atctcccagg acatcagtac ccgtgatgaa
     3601 ggcgggggcg gaaaggtcgt ggttatcggg cggcaggggt aaaaagaata aaaaaatccc
     3661 gcagtgatcg cggacaggaa ctgcgggaga gttacgaaga ttaagtgtaa ggaattattc
     3721 ttatgtcacg acaaaaaaat taacgcagag aaattataag tgccacaggg attttgtgaa
     3781 aatgtgaaga tattcagagt ttttctgttt gttcttctgg cgagagcgga ttcattgact
     3841 ggtggagaaa aggtaagggg gcagctcccc ttaccagact tgttatggga tgataccaca
     3901 gcccattctt gcaccgccgc cgccgagtgg atctggatgg tcatgatagt tgtcaccgcc
     3961 aatgtggatc ataagagacc gtccgcttat ttctgttaat tctttcagtt ttggtgcgag
     4021 aaccggatag tttgcttttc cgtcatgagt cacgtacaga gccgggagat cgcccaggtg
     4081 accatcagga gaccagggac caagatgttt tccagtctgt ttcgggtcaa agtgaccgcc
     4141 agctgctaat gccgcaacag atactccatc tttcagagcc ggcgcgcaac ttcccttttc
     4201 gtgcacatga aatccatgaa tgccctgagg taaagagtgc agatctggtg tgaacaggag
     4261 accatagctg gtctcctgga tagttattgt tccggcgctc acctctttac catcggcatt
     4321 aacaaggttc atggggactt ttattgtggc ggcatatcca catgatgttg ccagcaccat
     4381 tgcaatagca gcaagacttt tacggttcat aaaaccctca ttaattattt taacagactg
     4441 agtttactga ctgcaggagt acaaacagcg ttctgatgat atcgcgcaat agttattgaa
     4501 tgtgactatt gcttcgattg atcatatcaa ttaacaggag ttatcatggg taaaggtggc
     4561 ggcaaggcgc acacacctcg tgaggcgaag gataatctca aatccacgca gatgatgagc
     4621 gtgattgatg cgattggtga gggaccgata gaaggtccgg tgaagggact gcagagtatc
     4681 ctggtgaaca aaaccccgct gacggacacg gacggtaatc ccgtgataca cggtgtgacg
     4741 gcggtctggc gcgccgggga gcaggagcag acaccaccgg aaggctttga gtcctccgga
     4801 gctgaaactg tactgggcgt ggaagtgacg aaggcaaaac cggtgacgcg caccattacg
     4861 tccgcgaaca ttgaccgcct gcgggttacc ttcggggtgc agtcactggt gcagaccacg
     4921 tcaaagggtg accgaaaccc gacatccgtc cgcctgctga ttcagttaca gcgtaacggt
     4981 aactgggtga cagaaaagga tgtcaccatt aacggcaaga ccacctcgca gtttctggcg
     5041 ttggtgattc tggataatct gcctccccgt ccttttaaca tccggatggt ccgggagacg
     5101 gcggacagca ccacggacca gttgcagaac agaacactct ggtcatcgta caccgaaatc
     5161 atcgatgtga aacagtgcta cccgaacacg gcgattgtgg ggctgcaggt ggatgcggag
     5221 cagttcggtg gtcagcagat gacggtgaac taccatatcc gcggtcgcat catccaggtg
     5281 ccgtcaaact atgacccgga aaaacgcacg tacagtggta tctgggacgg cagtctgaaa
     5341 ccggcataca gtaacaatcc ggcctggtgt ctgtgggaca tgctgactca cccgcgctac
     5401 ggcatgggaa aacgtctggg ggcggcggat gtggacaagt gggc
//
LOCUS       DAZGJT010000185         5408 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZGJT010000185 DAZGJT010000000
VERSION     DAZGJT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.190"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..1485
                     /gene="mltD"
                     /locus_tag="ACHKFK_004628"
     CDS             127..1485
                     /gene="mltD"
                     /locus_tag="ACHKFK_004628"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO9054794.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1534..2292)
                     /locus_tag="ACHKFK_004629"
     CDS             complement(1534..2292)
                     /locus_tag="ACHKFK_004629"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9054795.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLADYPDASV
                     LDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEARQLKNITTRQGYVESLPF
                     ADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGLLIVMDVMSPGHPVRDIWLQTVE
                     ALRDTSHIRNHASGEWLTLISEANLIVDNLITDKLPLEFSSWVARMRTPEALVDAIRI
                     YQQSASTEVKMYFALQNDGSFTSDIIMLEAHKAA"
     gene            complement(2370..3170)
                     /locus_tag="ACHKFK_004630"
     CDS             complement(2370..3170)
                     /locus_tag="ACHKFK_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO9054796.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3411..4325
                     /gene="yafC"
                     /locus_tag="ACHKFK_004631"
     CDS             3411..4325
                     /gene="yafC"
                     /locus_tag="ACHKFK_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO9054797.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFHRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISCYGKPETIDDLKQHICLRFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSGHVKTAPGGAVREA"
     gene            complement(4322..5125)
                     /gene="dkgB"
                     /locus_tag="ACHKFK_004632"
     CDS             complement(4322..5125)
                     /gene="dkgB"
                     /locus_tag="ACHKFK_004632"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO9054798.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPS
                     DEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5288..5364)
                     /locus_tag="ACHKFK_004633"
     tRNA            complement(5288..5364)
                     /locus_tag="ACHKFK_004633"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5328..5330),aa:Asp,seq:gtc)
BASE COUNT         1421 a         1304 c         1346 g         1337 t
ORIGIN      
        1 gcaacaacct gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
       61 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
      121 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagc
      181 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
      241 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
      301 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaacga ccggattcgc
      361 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
      421 gagccgtata tgtactggat agccgggcaa gttaaaaaac gtaacatgcc tatggaactg
      481 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
      541 gcgggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
      601 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
      661 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
      721 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
      781 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
      841 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
      901 gctctggcac gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
      961 gggatttccg tcagcaagct gaagacattc aacgcaggcg tgaaaggctc cacgctgggc
     1021 gcaagtggtc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgaatct
     1081 ctggcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     1141 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagtatcgc ttcacgtctc
     1201 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     1261 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc gactggcaaa caacagcgat
     1321 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     1381 gtgaacatca aagatgtaat gcgctggaac agcgacactg cgaatctgca accaggcgat
     1441 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     1501 aaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgtg cttctagcat
     1561 gatgatatca ctggtgaaag agccatcatt ctgcaaggca aaatacattt tcacctctgt
     1621 cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct
     1681 cgctacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag
     1741 attggcttca ctgattaacg tcaaccactc accgctggcg tggttccgta tgtgagatgt
     1801 atcgcgtaat gcttctaccg tctgtaacca gatgtcgcgc actgggtgac ccggagacat
     1861 tacgtccatc acaatcagca gaccaccggg tttcaatatc cggttcactt cacgcagtgc
     1921 tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt
     1981 atcggcaaat ggcaggcttt cgacataacc ctggcgggtg gtgatatttt tcagttgccg
     2041 ggcttcggca gcttgtgcca cgacatccag catatgggca gataagtcat acgccaccac
     2101 cgtgctcacg ttttgcgcag cgacaaagct ggcatgccct gctccgcagc ccatatcaag
     2161 tacacttgca tcaggataat cagccagacg ctgtaaatct cgtccggatg catgtacggt
     2221 actagtcaga tattcgctag cttgagagct aaactgcttt tctacgtggt catggtggga
     2281 ttgtgttgtc attgcgctgt ccttttgtta tttgagtata aaagggcagc gccaaacgtt
     2341 ggtctgccct gtggcagacc tgacatacct tatttatcag gcttgccggg actgaattca
     2401 acgagtagcg gattgtgatc ggacgcgcgc gtaaccagta cagaagcttc gctgacgttc
     2461 agaccacggt agaaaacaaa atcgagcggg cgaccaaacg cccgacggcg ctgatcatcg
     2521 gtaaaacgca cctggcgcag cgacatttcc cgtgcaaagc gatataacgc gttcatcctt
     2581 ctacggctcc aggcattgaa atctcccgcc ataatgaccg ggccactgtg gtgagctatc
     2641 tgatcgccaa taggaagtaa ctgcttacta tagacatcca cgcccagact gaagttgacg
     2701 gcgtgtatat taaccaccat caacaggcgg gtgtcaggta atggatagac cgtcaccagt
     2761 gccgacttcg ccagacgcaa aatgggttct cgttcacgta acgggcagca atacactgga
     2821 tgtgccgccg aaagggtcat tacgccagaa ggatgttgtg gcagcacgaa agcgggtacc
     2881 tgatcggcgg caagatagtt agcagtcgca aactgtacta actctggcgt tgtctgcgct
     2941 tcctgcaata acaccagatg tgcatccttg ccgtagttct ttaataccga caaccattca
     3001 gcgcgttgct gtttgtatat gttccacacc aggatccgaa tacgctcttc ggtacttaac
     3061 ggttctccag gtggtaatgc ctggccgatg ctcgcaaaag accctggcgg taagatcctt
     3121 tccgcaggtt gtccggcaac atagcgcatg gcataggtgt tttttcgcac ttttgacttt
     3181 gaaacctcta tcacgtcaac ccttccgcac tcgggagggt gctactgtta tagggatttt
     3241 agctcactct ttcaacgagc aattgtatat tgttatgtaa gcaagtgctt acaccggtca
     3301 ggattttgct cattattgag aacattaaat cggatggcat atctcgcatc ctgatggttt
     3361 aatgacaaat aaattcaaaa tatctcttct attttgcaac aggagcaaaa atgaaagcca
     3421 cgtcggaaga actcgccatt tttgtttcag tcgtcgaaag cggcagcttt agccgggcag
     3481 cggaacaatt agggcaagca aactcagcgg taagccgggc ggtgaaaaag ctggagatga
     3541 aacttggcgt tagcctgctt aatcggacca cgcggcaact tagcctgacg gaagaaggcg
     3601 agcgttattt tcatcgcgta cagtcaattt tgcaggagat ggcagcggca gaatcagaaa
     3661 ttatggagac gcgtaataca ccgcgtggac tgttacggat cgatgccgca actccagtgg
     3721 tgctgcactt tctgatgccg ttgattaagc cttttcgtga acgctatccg gaagtcactt
     3781 tatcgctagt ctcctccgaa acgattatta atttgatcga aagaaaagtg gatgtcgcga
     3841 tacgcgccgg tacgttaacg gattccagct tacgtgccag gccgttattt aacagttatc
     3901 gaaaaattat cgcctccccc gattatattt cctgctacgg gaagccagaa acgatcgacg
     3961 atttaaagca acatatttgc ctgagattca ctgaacccgc ttccctcaat acctggccga
     4021 tagcctgtag cgatggacaa ttacatgagg tgaagtacgg tttgtcatcc aatagtgggg
     4081 aaacactgaa acagctttgc ctgagtggca acgggattgc gtgtttgtcc gactacatga
     4141 tcgacaaaga aatcgctcgc ggagaattgg tggagttaat ggcagataaa gtgttgccag
     4201 tggaaatgcc attcagtgca gtctattaca gcgaccgtgc ggtaagtacg cgcatccggg
     4261 cttttatcga tttccttagc ggacatgtaa aaacagctcc cggaggagct gtcagagagg
     4321 cttaatccca ttcaggagcc agaccttccg ggctaaccag gcggtcgttg caatccagtg
     4381 cggcgatcgc ttttttatct tcggcatcaa gctgtaaatt ttgtgcctta agattactct
     4441 ccaagttttc acgtttagta gaagaaggaa ttactgagta accttccccc atagcccacg
     4501 ccagaatcac ttgagccgga gtcgcattgt gtttagctgc aatacgagca ataaccttat
     4561 ctttcagtgc attgccatac gccagagtca tataggaagt aatatggata ccgtgctgtt
     4621 tagcccaagc gaccacttta cggttttgca gataaggaga gagttcaatc tggttagtag
     4681 cgatgttttc agcgccaaca gcagcaatcg ctttttccat caatgggatc gtgaagttgg
     4741 aaataccgat ctcacgcgtc agcccttgtt ttttggcttc cagcagcgcc tgcataaact
     4801 cttcagcaga gacttcatcg cttggtgacg gccagtggat tagcgtcaga tcaacataat
     4861 cggtacgcaa tttttgcagg ctctctttca gactcgggat caatttgtct ttgctgagat
     4921 tttcaatcca gattttagtg gtgatgtaga gttcatgacg tggcacgcca ctttctgcaa
     4981 tcgcctgacc tactgcggct tcgttatcat agatttgtgc ggtatcaatt gcgcgataat
     5041 caagttcaag cgccgttttc acagatgaaa taacaacgtc gtctttcaga cggaaagtgc
     5101 ctaaaccaaa tgcagggata gccataatat gcctctttta atgctatttt cgttaactga
     5161 ggtgattatg ggcttcagac agttgtggaa aaatggcgta aaacgcagag gatttttgcg
     5221 attttggcaa taatagaaat acaaacgaaa aaaaacctcg agcgttaact cgaggcttct
     5281 taataagtgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat
     5341 tctaaccgac tgaactaccg ctccaccgaa ttcttttaca accaccggtt tatgaccggc
     5401 ttactgct
//
LOCUS       DAZGJT010000186         5366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZGJT010000186 DAZGJT010000000
VERSION     DAZGJT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.191"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..1600)
                     /gene="yeeO"
                     /locus_tag="ACHKFK_004634"
     CDS             complement(113..1600)
                     /gene="yeeO"
                     /locus_tag="ACHKFK_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO9054799.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAVAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1761..1836
                     /locus_tag="ACHKFK_004635"
     tRNA            1761..1836
                     /locus_tag="ACHKFK_004635"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1794..1796,aa:Asn,seq:gtt)
     gene            complement(1874..2824)
                     /gene="cbl"
                     /locus_tag="ACHKFK_004636"
     CDS             complement(1874..2824)
                     /gene="cbl"
                     /locus_tag="ACHKFK_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO9054800.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2926..3843)
                     /gene="nac"
                     /locus_tag="ACHKFK_004637"
     CDS             complement(2926..3843)
                     /gene="nac"
                     /locus_tag="ACHKFK_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO9054801.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLNRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4170..4245
                     /locus_tag="ACHKFK_004638"
     tRNA            4170..4245
                     /locus_tag="ACHKFK_004638"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4203..4205,aa:Asn,seq:gtt)
     gene            complement(4301..5236)
                     /gene="ldtA"
                     /locus_tag="ACHKFK_004639"
     CDS             complement(4301..5236)
                     /gene="ldtA"
                     /locus_tag="ACHKFK_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO9054802.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPDTVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWLTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5298..>5366)
                     /locus_tag="ACHKFK_004640"
     CDS             complement(5298..>5366)
                     /locus_tag="ACHKFK_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054803.1"
                     /translation="NMGELAASNIVLPGNTTSDLNS"
BASE COUNT         1508 a         1394 c         1160 g         1304 t
ORIGIN      
        1 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
       61 gcctgttttt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
      121 acacaactgg ctttttttca cacttttgcg gctcgggtcg agggtatttc catagccaac
      181 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
      241 ccatccagac accaaccaca ccccagccaa gcatgattcc cagcacataa ccgactacaa
      301 cccgacaacc ccacatgctc aacatcgaaa cccacatagc gtaacgggca tcacgagcac
      361 ctttaaatcc agcgggtagc acccatgagg cggaccaaat aggcataaat aaagcattta
      421 gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca
      481 taagcccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggacagcc
      541 agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc
      601 ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa
      661 ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt
      721 gggttaataa ccgaccactg gtaaataaca ctgattcgac actagcggga ataccaatcc
      781 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
      841 ttagcgcagg attaaaacca atcgctagca cccacaaaat tgcaactgcg ccaatataac
      901 gagaaatggt taaacccagc cctgccccga caaatcccag tcccggccag gagaaaaggc
      961 cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg ttaatcagta
     1021 gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaatcaaa gtgatggcaa
     1081 ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtgct ttaacttctg
     1141 tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc aaaatgatga ataagcgttg
     1201 ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc
     1261 gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag
     1321 cagcaaaaaa agccataatg accatattga agctgtccgc caatcccacg ccggccatcg
     1381 catcttttcc cagccagctg accagaaaag tgctcaggac acccatcaac aggacacagg
     1441 cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt
     1501 agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga
     1561 tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat
     1621 cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata
     1681 gaaacggcaa acagttggga aatttaaaaa tcgggtttga caaaagattt ttcgccgtta
     1741 agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     1801 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt tctgaaaagc ccgcttttat
     1861 agcgggcttt ttgctatatc tgataatcaa tttcctcttc actgttctcc atcacctggc
     1921 gcttgatatc ctcaactgac agtcctgcat tacaaagttc cagaaagcgc cagacatagt
     1981 tacgctgaag ttgccctcgc ttcagtccca accatacagt attggcatcg aaaagatgcc
     2041 gcgtatccag acggattaaa ttctcttcct cttgttcgcc actggattgc tcggcaacta
     2101 atccgatccc aagcccaaga gcaacatagg ttttaatgac atcagaatcc tgcgcactta
     2161 atacaatatc tgccagcaaa cctttgcggg caaatgcgtc atcaatacgt gagcgccccg
     2221 taatcccctg tcggtaagtg attaacggcc acttcgctat tgattccagc gtcaatggtg
     2281 aaatttgcgt caagggatga tcgtgtggaa caagcaaact atggtgccaa cgaaaccacg
     2341 ggaaggcgac gagctgcggg tcattactca aacgctcgct ggcgatacca atatcagctt
     2401 cgccattttg caacaatgtc gcaatttcct gtggcgtccc ctggattagc tcgagccgaa
     2461 cctccgggaa aagttcgcga aaagctttaa tgacctctgg caagctataa cgtgcctgag
     2521 tatgcgtcgt tgcaatagtg agaacgccag acgtatcgtt ggtaaacagg tctgcaagcc
     2581 gacgaacatt actggcttca ttcagaatac gttctgcaat gaccagtaat gctttgcccg
     2641 gttcagtcat gcccagcagt cgcttacctc gtcgaacaaa tatttcgatg ccaagttcat
     2701 cctccagttc ccgaatatga cggctgacgc ctgactgtga ggtaaaaagc atattcgcaa
     2761 cctctgtcag gttgtaatcc tgacgtgcag cctcgcggat tatctttagt tgttggaaat
     2821 tcacggtaaa ctccgggcag ttcagatttc ccgttattgt taaagtctaa tgcccggcat
     2881 aacaaataat aaaaacccgc atcttattcc atcccgatat aacacttagc tcaccaattg
     2941 ccactgcctt ttttccatca ctggagaact aatcactgac attaacaact ctttcactgc
     3001 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
     3061 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
     3121 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
     3181 agactcaatt tcaccaataa cttttgccgt gagtcgccgt agggaaaaag cctcatcaac
     3241 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcatttgcg caatagcatt
     3301 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
     3361 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
     3421 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
     3481 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
     3541 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
     3601 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
     3661 tccggcgtct gttggtgtaa cgccccgctt tgtacggttc aaaagttgtt gatttaactc
     3721 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
     3781 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
     3841 catgttgcct ccgtttttta agaatcggcc caagtgccgc cattacttac aaccagattg
     3901 caagatgctt gccagtttta ttttggtgtt gatgtacaag ctaaccaact gtcaaataag
     3961 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     4021 aaaggggggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     4081 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     4141 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     4201 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     4261 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     4321 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     4381 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     4441 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     4501 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     4561 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     4621 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     4681 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     4741 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     4801 cttcttgttt acgttcaacg gtagtcaacc agttacgcgg ggtttctcgc ccagcctggc
     4861 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     4921 tctcagcgac gttaacaaca atccctttac gaacagtgtc gggcaaaatc agttgctgcg
     4981 gaatggtgag ttgcgagcca gacttcggca aaaaaacatc agcgcccggg ttcgcttcca
     5041 gcatgttact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt
     5101 gatcaggaac agttacagta agcgactgcc ccaccaaacg gctaccctct ggaggtaatg
     5161 gataagttac cgccaggcta gtatggctgg caaaaagcag agcaaatgag caaagaatat
     5221 ttacacgacg catcatgtcc ctttcctatg tcgcgaaagc tatccgttaa gtatagcttt
     5281 tatcagactt ttcgttttta actgttcaaa tcagaagtcg tattccccgg tagaacaata
     5341 ttactggcag caagttcgcc catgtt
//
LOCUS       DAZGJT010000187         5339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZGJT010000187 DAZGJT010000000
VERSION     DAZGJT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.192"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(330..1220)
                     /gene="rarD"
                     /locus_tag="ACHKFK_004641"
     CDS             complement(330..1220)
                     /gene="rarD"
                     /locus_tag="ACHKFK_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO9054804.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(1272..1739)
                     /gene="yigI"
                     /locus_tag="ACHKFK_004642"
     CDS             complement(1272..1739)
                     /gene="yigI"
                     /locus_tag="ACHKFK_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO9054805.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNKPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1904..2773
                     /gene="pldA"
                     /locus_tag="ACHKFK_004643"
     CDS             1904..2773
                     /gene="pldA"
                     /locus_tag="ACHKFK_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO9054806.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYIIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSDESSPFRETNYEPQLFLGFATDYNFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2898..4727
                     /gene="recQ"
                     /locus_tag="ACHKFK_004644"
     CDS             2898..4727
                     /gene="recQ"
                     /locus_tag="ACHKFK_004644"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO9054807.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQISLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4791..>5339
                     /gene="rhtC"
                     /locus_tag="ACHKFK_004645"
     CDS             4791..>5339
                     /gene="rhtC"
                     /locus_tag="ACHKFK_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO9054808.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEVVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRL"
BASE COUNT         1336 a         1318 c         1373 g         1312 t
ORIGIN      
        1 agcctaatat gttgttcatt tatattctcc tttcttataa ttaagggcaa tgttaatttg
       61 ataagaaata atagggtaaa aaaattttaa aaaagaaatg cacttcacat tttttattac
      121 attctgaaga gaacttggtt aatgattggt cttgctcatc gctaataaca taatgctgaa
      181 tttaccaaaa atgaaattta ttaaagcaaa tgaaaatata caacgacgtc tggaaattct
      241 gcctttcctt atgtgagtct cctgtcagca tggcaatatg cccactctca tgcaagagtc
      301 ggcatatttt ttcagaatat atttattttt tatttggacg ttctacgctg cgtataaatc
      361 gcatccatca caaaaattgc cagcgccacc caaataaagg cgaaagttac catcttatcg
      421 gcacccggtt tttcaccata aaacgtcaca gccagcagga acatcagcgt cggaccaatg
      481 tactggaaaa agcctaacgt tgagagacgc aggcgtgtcg ccgctgcagt aaaacacaac
      541 agtggcacgg tggtgacaat accggcggcg atcagcagta aattcagcga catcgggttt
      601 tgccccatat gactggttga gctgtcggca atagcaaaca ggtaaatcgc cgccacgggc
      661 agcaaccaca tagtttcgat taacatgccg gtttgcgctt caacagcaat cttcttgcgc
      721 accaggccgt agaaagcaaa actaaacgcc agccccagcg cgatgatcgg cagtgaacca
      781 aaagtccaca actgaaccag cactccacat atcgccagaa tcaccgccag ccattgcatt
      841 cggcggaatc gctcgccgag gaaaatcatc cccagcacaa tgttcaccag cgggttaata
      901 aagtaaccaa ggctcgcttc cagcatatgg tgattgttca ccgcccagat aaacagtagc
      961 cagttgccgc caatcagcac ggcagagact gccagcataa aaattttctg tggcgtctga
     1021 atcagcgttt ttaaatagga ccactggcgg caaatgctca tcagcaccac cataaagaaa
     1081 aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg gcacgtagta aatcagtttg
     1141 aagtacgctg gcgcaatacc ccaaataaaa taagcggcaa gagcgagtaa tacgccctgc
     1201 cgcgtttgtt ttgcatccat cgggaatact catttttaat tggtaacagc agtttacctg
     1261 cttttatgtc ttcaacctac catataggtg gcggtggcac tggcaatata aagctgttct
     1321 tcattgtgta attcaacgcg ggcgacggcg actttattgc ctgcacgtaa caggctactg
     1381 gtagcagtaa aacgctcgcc tctgcctggg cgcagataat cgacgcgaag atcaatggtc
     1441 cccatccgcg atagccgctg gcgtagttca tcttcactga tggtttcgtg gcgggttaag
     1501 gtacttccca cgcacaccag acctgcggcg acatccagcg ccgacgcaat gaccccgccg
     1561 tgtaaaatgc tttgcgccca gttgcccacc atcatcggct tatttttaaa ggccagctgt
     1621 gcgaactctt tttcgtaacg ctccagttcc atccccaatg cgcggttaaa tggcatgtga
     1681 taaacaaaca tctcacccac taatttgagg gcttgttcag cggtcagtac ggcagacata
     1741 acatccttac acttcattag ttaatggaat gttgatttta tgcttattta ttgttggttt
     1801 ctactttagg aagggataac taacggctat ggagttaagt atgtaaaata gcccgcagaa
     1861 aaatattcac cttatcaata attcgttacg gagaacacga gcgatgcgga ctctgcaggg
     1921 ctggttgttg ccggtgttta tgttgcctat ggcagtatat gcacaagagg caacggtgaa
     1981 agaggtgcat gacgcgccag cggtgcgtgg cagtattatc gccaatatgc tgcaggagca
     2041 tgacaatccg ttcacccttt atccttatga caccaactac atcatctaca cccaaaccag
     2101 cgatctgaat aaagaagcga ttgccagtta cgactgggcg gaaaacgcgc gtaaggatga
     2161 agtaaagttc cagttgagcc tggcgtttcc gctgtggcgt gggattttgg ggccaaactc
     2221 tgtactgggc gcctcctaca cacagaaatc ctggtggcag ctttctaata gcgatgagtc
     2281 atcaccgttt cgtgaaacta actacgaacc gcaattgttc ctcggttttg ccacggatta
     2341 caactttgct ggctggacgc tgcgcgatgt ggaaatgggc tacaaccacg actcaaacgg
     2401 gcgttcagac ccgacttccc gcagctggaa ccgcctttat acccgcctga tggcagaaaa
     2461 cggtaactgg ctggtagagg tgaagccgtg gtatgtggtg gggaatacgg acgataaccc
     2521 ggacatcacc aaatatatgg gttactacca gcttaaaatc ggctatcacc tcggtgatgc
     2581 ggtactgagc gcgaaagggc agtacaactg gaacaccggt tacggcggcg cggagttagg
     2641 cttaagctac ccgatcacca aacatgtgcg cctttatact caggtttaca gcggttacgg
     2701 cgaatcgctc atcgactata acttcaacca gacccgcgtt ggtgtgggag ttatgctaaa
     2761 cgacctgttt tgatgaatgg ttggcaaatc tggaatccag catccaggat taccctctca
     2821 gagactaaaa gcattgcagt ttctcgcgca ggcgctgaaa atagcgcctg tttttatttc
     2881 aggcaatcgg ggtgaatgtg gcgcaggcgg aagtgttgaa tctggagtcc ggagctaaac
     2941 aggttttaca agaaaccttt ggctaccaac agtttcgccc cggccaggaa gagattatcg
     3001 acaccgtact ttccggtcgc gattgcctgg tcgtcatgcc cactggtggc ggaaaatccc
     3061 tttgctatca aatccctgcc ttattgctaa acggccttac cgtggttgtc tcaccgctga
     3121 tttcgttgat gaaagatcag gtagatcaac tgcaagccaa cggcgtggcg gcggcgtgcc
     3181 ttaactcgac gcaaacccgc gagcagcagc ttgaagtgat gacaggctgt cgcaccgggc
     3241 aaatcagcct gctttatatc gcgccggaac gcctgatgct ggataacttt cttgagcatc
     3301 tggcgcactg gaatccggtg ttattagccg ttgatgaagc gcactgtatc tcccaatggg
     3361 gtcacgattt ccgcccggaa tacgctgccc tcggtcagct gcgtcagcgt tttccgacgc
     3421 tcccgtttat ggcgctgact gccacagccg atgacaccac gcgccaggat atcgtgcgcc
     3481 tgctggggct gaacgatccg ctgattcaaa tcagcagttt tgaccgtccg aatattcgct
     3541 acatgctgat ggagaaattc aaaccgctcg atcagttgat gcgttatgta caggaacaac
     3601 gcggtaagtc ggggattatt tactgtaata gtcgtgcgaa agtagaagac accgctgcgc
     3661 gcctgcaaag caagggaatt agcgcggcgg cctatcatgc cgggctggaa aataatgttc
     3721 gcgctgatgt gcaggaaaaa ttccagcgcg atgacctgca aattgtggtg gcgacggtgg
     3781 cgttcggcat gggcatcaac aagcctaacg ttcgcttcgt ggtgcatttt gatattccgc
     3841 gcaatatcga atcctattat caggaaactg gacgcgccgg gcgtgatggc ctgcccgcgg
     3901 aagcgatgct gttttacgat ccggctgata tggcgtggct gcgccgttgt ctggaagaga
     3961 agccgcaggg gcagttgcag gatatcgaac gtcataaact caatgcgatg ggcgcgtttg
     4021 ctgaagcgca aacttgccgt cgtttggtat tgctgaacta ttttggcgaa gggcgtcagg
     4081 agccgtgcgg gaactgcgat atctgcctcg atccgccgaa acagtacgac ggttcaaccg
     4141 atgcgcagat tgccctttcc accattggtc gtgtgaatca gcggtttggg atgggttatg
     4201 tggtggaagt gattcgcggt gctaataacc agcgtatccg cgactatggt catgacaaac
     4261 tgaaagtcta tggcatgggc cgtgataaaa gccatgaaca ttgggtgagc gtgatccgcc
     4321 agctgattca cctcggcctg gtgacgcaaa atattgccca gcattctgcc ctacaactga
     4381 cagaggctgc gcgtccggtg ctgcgcggcg aatcctcttt gcaacttgcc gtgccgcgta
     4441 tcgtggcgct caaaccgaaa gcgatgcaga aatcgttcgg cggcaactat gatcgcaaac
     4501 tgttcgccaa attacgcaaa ctgcgtaaat cgattgccga tgaaagcaat gtcccgccgt
     4561 acgtggtgtt taacgacgca accttgattg agatggcgga gcagatgccg atcaccgcca
     4621 gcgaaatgct cagcgttaac ggcgttggta tgcgcaagct ggaacgcttt ggcaaaccgt
     4681 ttatggcgct gattcgtgcg catgtcgatg gtgacgacga agagtagtca gcagcataaa
     4741 aaagtgccag gatgaagact ccgtaaacgt ttcccccgcg agtctaatgt atgttgatgt
     4801 tattcctcac cgtcgccatg gtgcacattg tggcgcttat gagccccggt cccgatttct
     4861 tttttgtctc tcagaccgct gttagtcgtt cccgtaaaga agcgatgatg ggcgtgctgg
     4921 gcattacctg cggcgtaatg gtttgggctg ggattgcgtt gcttggccta catttgatta
     4981 tcgaaaaaat ggcctggctg catacgctga ttatggtggg cggtggcctg tatctctgct
     5041 ggatgggtta ccagatgcta cgtggtgcac tgaaaaaaga ggttgtttct gcccctgcgc
     5101 cacaagtcga actggcgaaa agcgggcgca gttttcttaa gggcttactg accaatctcg
     5161 cgaaccctaa agcgattatc tactttggtt cggtgttctc gctgtttgtc ggtgataatg
     5221 tcggcaccac cgaacgctgg ggcattttcg cgttgattat tgtcgaaaca ctggcgtggt
     5281 ttaccgtcgt tgccagcctg tttgctctgc cgcaaatgcg ccgtggttat caacgtctg
//
LOCUS       DAZGJT010000188         5240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZGJT010000188 DAZGJT010000000
VERSION     DAZGJT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.193"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..154)
                     /locus_tag="ACHKFK_004646"
     CDS             complement(<1..154)
                     /locus_tag="ACHKFK_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO9054809.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILI"
     gene            complement(269..580)
                     /locus_tag="ACHKFK_004647"
     CDS             complement(269..580)
                     /locus_tag="ACHKFK_004647"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO9054810.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHAIAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            779..1477
                     /locus_tag="ACHKFK_004648"
     CDS             779..1477
                     /locus_tag="ACHKFK_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO9054811.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1522..2421)
                     /gene="eamA"
                     /locus_tag="ACHKFK_004649"
     CDS             complement(1522..2421)
                     /gene="eamA"
                     /locus_tag="ACHKFK_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO9054812.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHATRPAVMSLVIWSALIPIIPFFVASLILDGSATMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFFGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2616..3803
                     /gene="ydeE"
                     /locus_tag="ACHKFK_004650"
     CDS             2616..3803
                     /gene="ydeE"
                     /locus_tag="ACHKFK_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO9054813.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMHSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGIVLTSLPPFSLFIILALVIVVAWVLMLKGIRARPWGQP
                     ALC"
     gene            3930..4025
                     /gene="mgtS"
                     /locus_tag="ACHKFK_004651"
     CDS             3930..4025
                     /gene="mgtS"
                     /locus_tag="ACHKFK_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO9054814.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            4025..4129
                     /gene="mgtT"
                     /locus_tag="ACHKFK_004652"
     CDS             4025..4129
                     /gene="mgtT"
                     /locus_tag="ACHKFK_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO9054815.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(4244..5134)
                     /gene="dgcZ"
                     /locus_tag="ACHKFK_004653"
     CDS             complement(4244..5134)
                     /gene="dgcZ"
                     /locus_tag="ACHKFK_004653"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO9054816.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASEPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSSMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKATN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT         1442 a         1243 c         1088 g         1467 t
ORIGIN      
        1 taattaatat aattaatgag cttgccatga taattggaaa cgtaaaaacg aatccatctc
       61 tcaccgctgc aacatgtttt tgtgagttta acttgatagc aactggaaca agaaaacgtt
      121 ccattccacg ttcgaatgat gccattaatc ccattttttt atacctgttg tcctggatat
      181 ttatacatcc caataaagca tgtatgccat atccatttta tgatgtgtga aaagttagtt
      241 caggtaatag tttaataata ttagattctt actcttgcag tagtttcatc gcaaattcaa
      301 gaactgcctg tccgttcatt gtgccatagt ctttcatctc gataacggct acaggcattt
      361 tcccctgtgc aacggcttca atctctggtt tctgaaagcg aacttgcgga ccaagtaaaa
      421 caacgtcaac ttcgttattt ttaattttcc ctttagcctc tgcaatcgcc aatgcggaaa
      481 tattaacttc aagtgaaata gcgatagcat gatcaatcat acgtttaacc agcatactgg
      541 ttgacatgcc cgcagcgcaa actaacaata tctttttcat atttttcctt actggtatat
      601 aacagactac attgtgtaaa tatcatatcc cacagtagct acacgtcctg gtacattgat
      661 cacagtaata cagcaataaa gttgttcaac aacagggtga taattaaacg actcggccat
      721 acgataaact aatcaggtct caactccttg ttttcaatgg ctcttaaaag gtgcaaaagt
      781 gatttttcaa aagattgccc gcttacttaa atccgagatc aatggcaatt catggcatgt
      841 tggtgatttg ttgccgtcag aagcggaact cgctgttcgt tataatgttt cgcgtaatac
      901 cctgcgtaag gcattatccc tgctggaagg cgaaggaatt attcacagaa agcatggttc
      961 aggaacatac attcaaaaaa agaattttgt tgcacacatt gatcacatga acagtttcag
     1021 tgaaattgca cataaaagcg gcaaagaggc aggaagtcag attatgaaat ttgaagtgca
     1081 ggatgcttct cctactattg cgactgagct gaatttagtg actggtgagc aggtttatta
     1141 cataaagaga ctgcgattca ttgaggataa tgcagcacaa ttagaagaaa cgtggatgtc
     1201 agtggcacgt tttcctgatt taaccgtatc acatatgcaa aaatccaaat tttcgtatat
     1261 tgagaacgaa tgcgggatca aaatcattgg cacctttgaa actttctccc cgacttttcc
     1321 taccccagaa atcgccagta ttttacggat cagcccacgg gatcccatac ttaaaattca
     1381 gacccaggct gtggatagta actctattcc gctggattat tcgttacttt acagcaatat
     1441 tttcgagttc caggtaaagt acttttttcc gcgataaaag cagacgcaca gagtgctggc
     1501 atcacattct gtgcgtatgg tttagcctct gacctttacc gctttacgcc accgcaagcc
     1561 aaatacattg atatacagcc cggtcataat gagcaccgca ccgaaaaatt gcagacccgt
     1621 taagcgttca tccaacaata gtgccgcact tgccagtcca actacaggca ccagtaacga
     1681 taacggtgca acccgccagg tttcatagcg tcccagtaac gtcccccaga tcccataacc
     1741 aacaattgtc gccacaaacg ccagatacat cagagacaag atggtggtca tatcgatagt
     1801 aaccagactg tgaatcatgg ttgcggaacc atcgagaatc agcgaggcaa caaagaaggg
     1861 aatgattggg attaatgcgc tccagattac cagcgacatc actgccggac gcgttgcgtg
     1921 cgacatgatc tttttattga agatgttgcc acacgcccaa ctaaatgctg ccgccagggt
     1981 caacataaag ccgagcatcg ccacatgctg accgttcaga ctatcttcga ttaacaccag
     2041 tacgccaaaa atcgctaagg cgatccccgc caattgtttg ccatgtagtc gttcaccgaa
     2101 agtaaacgcg ccaagcacga tagtaaaaaa cgcttgtgcc tgtaacacca acgaggccag
     2161 tccagcaggc ataccaaagt taatagcgca gaacagaaag gcaaactgcg caaaactgat
     2221 ggttaatcca taccccagca gcaaattcag tggtactttc ggtcgtgcga caaaaaagat
     2281 ggccgggaaa gcaaccagca taaagcgcaa accggccagc atcagcggtg gcatgttatg
     2341 aagccccact ttgatgacca caaaatttag cccccataca accactacca gtagcgccaa
     2401 caccccatct tttcgcgaca ttctaccgcc tctgaatttc atcttttgta agcaatcaac
     2461 ttagctgaat ttacttttct ttaacagttg attcgttagt caccgtttac gacggcatta
     2521 atgcgcaaat aagtcgctat acttcggatt tttgccatgc tattccttta catctctaaa
     2581 acaaaacata acgaaacgca ctgccggaca gacaaatgaa cttatcccta cgacgctcta
     2641 ccagcgccct tcttgcctcg tcgttgttat taaccatcgg acgcggcgct acgctgccgt
     2701 ttatgaccat ttacttgagt cgccagtaca gcctgagtgt cgatctaatc ggttatgcga
     2761 tgacaattgc gctcactatt ggcgtcgttt ttagcctcgg ttttggtatc ctggcggata
     2821 agttcgacaa gaaacgctat atgctactgg caattaccgc cttcgctagc ggttttattg
     2881 ccattccttt agtgaataac gtgacgctgg tggtgctctt ttttgccctt attaactgcg
     2941 cctactccgt ttttgctacc gtgctgaaag cctggtttgc cgacaatctt tcgtccacca
     3001 gcaaaacgaa gattttctca atcaactaca ccatgctcaa cattggctgg accatcggtc
     3061 cgccactcgg cacgctgttg gtgatgcata gcatcaatct gcccttctgg ctggcagcta
     3121 tctgttccgc gttccccatg cttttcattc aaatttgggt aaagcgcagc gagaaaatca
     3181 tcgccacgga aacaggcagt gtctggtcgc cgaaagtttt attacaagat aaagcactgt
     3241 tgtggtttac ctgctctggc tttctggctt cttttgttag cggcgcattt gcttcatgca
     3301 tttcacaata tgtgatggtg attgctgatg gtgattttgc cgaaaaggtg gtcgcggttg
     3361 ttcttccggt gaatgctgcc atggtggtta cgttgcaata ttccgtgggc cgccgcctta
     3421 acccggctaa catccgcgcg ctgatgacag caggcaccct ctgtttcgtc atcgggctgg
     3481 tcggttttat tttttccggc aacagcctgc tattgtgggg tatgtcagct gcggtattta
     3541 ctgtcggtga aatcatttat gcgccgggcg agtatatgtt gattgaccat attgcgccgc
     3601 caggaatgaa agccagctat ttttccgccc agtctttagg ctggcttggt gctgcgatta
     3661 acccattagt gagtggcata gtacttacca gtctgccgcc tttctcgctg tttatcatct
     3721 tagcgttggt gatcgttgtt gcgtgggtgc tgatgttaaa aggaattcgt gcaagaccgt
     3781 gggggcagcc cgcgctttgt tgatttaagt cgaacacaat aagaatttaa ttcagccttc
     3841 gtttaggtta cctctgctaa tatctttctc attgagatga aaattaaggt aagcgaggaa
     3901 acacaccaca ccataaacgg aggcaaataa tgctgggtaa tatgaatgtt tttatggccg
     3961 tactgggaat aattttattt tctgggtttc tggccgcgta tttcagccac aaatgggatg
     4021 actaatgaac ggagataatc cctcacctaa ccggcccctt gttacagttg tgtacaaggg
     4081 gcctgatttt tatgacggcg aaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg
     4141 gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt
     4201 tgacacagta gcatcagttt tctcaatgaa tgttaaacgg agcttaaact cggttaatca
     4261 cattttgttc gtcaataaac atgcagcgat tccttccggt ttgcttaccc tcatacattg
     4321 ccctgtccgc tcttccaatg accacatcca gaggctcttc aggaaatgca cgactcacac
     4381 cagcagtcac ggtaatgttg atatgccctt cagaatgtgt gatggcatgg ttatcgacta
     4441 actggcaaat tctgacacct gcacgacatg cttcttcatc attagtcgct ttgacaataa
     4501 tgataaattc ttcgcccccg tagcgataaa ccgtttcgta atcgcgcgtc caactggcta
     4561 agtaagttgc gagggtgcgt aatactacat cgccgattaa atgcccgtag gtatcattaa
     4621 ccaatttaaa tcggtctata tccaacaaca ttaaataaag attcagaggt tcagcattgc
     4681 gtaactgatg atcgaaggat tcatcaagaa cccgacgacc cggcaatccc gtcagaacat
     4741 ccatactgct acggatcgtc agcaaataaa ttttgtaatc ggttaatgcc gcagtaaaag
     4801 aaagcaaccc ttcctgaaaa gcgtcgaaat gcgcgtcctg ccagtgattt tcaacaatag
     4861 ccagcattaa ttcccgacca cagttatgca tatgttgatg ggcagaatcc attagccgaa
     4921 cgtaaggtaa ttcatcgtta tcgagtggcc ccagatgatc aatccaccga ccaaactggc
     4981 acagtccata agaatggtta tccgttattt ctggctcact ggcatctctc gcgaccacgc
     5041 tgtgaaacat acttaccagc cactggtagt gggcatcgat agccttattg agatttaaca
     5101 agatggcatc aatttccgtt gtcttcttga tcattgccac tcctttttca cagttccttg
     5161 tgcgcgctat tctaacgaga gaaaagcaaa attacgtcaa tattttcata gaaatccgaa
     5221 gttatgagca tctctgagat
//
LOCUS       DAZGJT010000189         5171 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZGJT010000189 DAZGJT010000000
VERSION     DAZGJT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.194"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..446)
                     /locus_tag="ACHKFK_004654"
     CDS             complement(30..446)
                     /locus_tag="ACHKFK_004654"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO9054817.1"
                     /translation="MSVVIPLTVCLLNASAVHHIEPEILLSIISVEGGRVGAVSVNKN
                     GSEDLGIMQINTYAWLPLVSNTFFAGNQQIANEKLTNDGCFNIYVGTWILSKAISEEN
                     GDVWEGVGKYHSKTPSYKYKYIEKVKKEYERIKSYR"
     gene            complement(434..1165)
                     /locus_tag="ACHKFK_004655"
     CDS             complement(434..1165)
                     /locus_tag="ACHKFK_004655"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO9054818.1"
                     /translation="MYYFFIYWIGSVVASFICLAVERLPHQLKWREAPTPYLYIWYPP
                     SFCDFCKVRLKWYTLIPFFGYFLSKGKCNSCHTAISIKYPLIEFAVGLLSVVLFIFYG
                     NSHKFITLLCILYVLIFLTLIDIKEMWLPSVVTYPLFWGGLLCPDFCSDPLFRIYGAF
                     FCFVVTYCSMQLVGFLKNEDVFAGGDIALATAAGAWLGLEQSLFFVIYFSLIFIVYAL
                     PFRFFKVIYVPMGPAISLGFFLCLL"
     gene            complement(1170..2135)
                     /locus_tag="ACHKFK_004656"
     CDS             complement(1170..2135)
                     /locus_tag="ACHKFK_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO9054819.1"
                     /translation="MSLKSIYFADLLISNKGYHFRYLKGASNPITVVDEIYHAEIKKI
                     VAELNIRIDNGEHEFFYSYQNIPFRASIVETVDGVGYFLRRLKLPIPELDCLGFSPAM
                     LNTLKCLGNKRGMILIAGATGSGKSTTIYSLLKEYVSTFGDIVIAIEDPPEIPVQGCY
                     GDNSQGIWYQIDARQAGGYENAMISAMRYNPKYIFLGEIRSAKTAKEAIRAAVNGHLV
                     VSTIHGNTVQGAIYALQQIAASEGDIELVRSILGDGLLCVIHQELAFTSKGERILKAN
                     MLYVGGIPAIVSKIRSGKLELLSNEIESQKILLSRGINLASEQNR"
     gene            complement(2143..3213)
                     /locus_tag="ACHKFK_004657"
     CDS             complement(2143..3213)
                     /locus_tag="ACHKFK_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO9054820.1"
                     /translation="MPNFELAFAKMLFTSKLRMRIYEKLARYLANGVPLTFALDELYK
                     FTTDSGKRNKTPQAIAIHMWSISIRNGDSLTKALKGWVPEDELSILAAGEISGNLSDS
                     ISNIIYIYVTKKKVRAALFGIIYPIVLLLSTCLFLYIFGTKVVPAFEQVLPFTEWQGA
                     GRLMYYLAVFVQLYLMYLIAFLIMLTVIIIFTLPYWTGKIRSKFDLIPPWSIYRSVIG
                     CGFLLSLSSLITAGIPTPEAIRIISRNASPWYRERLTVIRLILLNGAPNIGEALYISG
                     FSFPSKNMVMDIRSYAALDGFEMMLERLSRQWQEESVEFISNQMNILKNAAILIMGGV
                     FMWIVSGMFSLQQQISNAAQFN"
     gene            complement(3206..4825)
                     /locus_tag="ACHKFK_004658"
     CDS             complement(3206..4825)
                     /locus_tag="ACHKFK_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO9054821.1"
                     /translation="MIVEQLKTADDQDFLRFRKNISDIVTVKGGLIETTDEQKKICII
                     YKNGDFLISTEHLENPSVRFLKEVAIRKGIQNITCYKVSLRDIRLLYQEQEKKVIQSS
                     SLPMEKIASDLIHECCDLRVSDLHIKVYTDEAEIFIRKDGDMFLLRQIEAEKGHSLLA
                     ALYNTAEDADSTYKLNSYQAARIIASKSRISIPERLQSIRLQFNPLGQGGRYMIARFL
                     YVEKTSITSIEALNPVKYGFHPVQAEAFKRLRQLPIGVNIISGPTGSGKSTTLKNILE
                     LLYIEKKAKVNIISIEDPPEYEITGTAQLPITNVDTEDERATEYRKAITAALRSDPDI
                     IMPGEARDAEVINLVFTAAMTGHQVWTSLHANSAVSIFDRLKDQGVDSFKLIDPELVT
                     GLVAQRLVKKLCPQCSLTYQEFTQSNVLSEIEENIIQGYHDSIRFPNNDGCSHCQSGY
                     SGRTIIAEIIEPDQIFLSLIDQGKRNEAVNYWQSSLSGITMRDHAWMKIISGEIYVLD
                     AVHKIAKLESLSNERREYLRKLCFLEDDYNA"
     gene            complement(4840..>5171)
                     /locus_tag="ACHKFK_004659"
     CDS             complement(4840..>5171)
                     /locus_tag="ACHKFK_004659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054822.1"
                     /translation="SGYEAQLKKFQELQRENFELKLKAQNEELKKKLGDNSIENVTVL
                     SIYSSGKSGYAAKVYGGGAGLRVVKTGEDINDSMHVTEINQRKVTIFLSKENKTLTLE
                     PYTIGGG"
BASE COUNT         1694 a         1051 c          791 g         1635 t
ORIGIN      
        1 atttaatcgt atagattagc tgaataaagt tagcgatagc tttttattct ttcatattcc
       61 tttttaactt tttctatata cttatactta tagctagggg ttttggaatg atatttacca
      121 accccctccc atacatctcc attttcctct gagatcgctt ttgaaagtat ccatgtgcca
      181 acatatatat taaaacaacc atcattggta agcttttcgt ttgcaatctg ctgattgcca
      241 gcgaaaaaag tattactaac aagtggaagc catgcataag tgtttatttg cataatacct
      301 aaatcctcac taccattttt attaactgaa actgctccaa cacgtcctcc ttcaacagat
      361 attatagata ataatatctc aggttcaata tggtgaactg cacttgcatt gagtagacaa
      421 actgttaatg gaattacaac agacataaga aaaatcccaa agaaatagct ggtcccatag
      481 gaacatatat aactttaaaa aatctgaatg gtaaagcgta aacgataaaa ataagagaaa
      541 aatatattac aaaaaaaagg ctctgctcaa gccccagcca agccccagcc gctgtagcaa
      601 gtgcaatatc accaccggca aaaacatcct catttttcag aaaccccaca agctgcatag
      661 aacaatacgt tactacaaag cagaaaaaag ccccataaat cctaaataat ggatcagaac
      721 aaaaatcagg acatagcaat cccccccaaa ataaaggata agtgacaact gacggtaacc
      781 acatttcttt aatatctatt aatgtcaaaa aaatcaaaac atatagaatg cataagagag
      841 taataaattt atggctatta ccataaaaga taaacagaac aacactcaac aaaccaacag
      901 caaattcaat tagtggatat tttatactga ttgctgtatg acatgagtta cactttcctt
      961 tcgataaaaa atatccaaaa aaaggaatta aagtatacca ctttaacctc actttgcaga
     1021 agtcacaaaa tgatggcggg taccaaatat atagataagg agttggggct tctctccatt
     1081 taagttgatg tggtaggcgt tcaacagcaa ggcaaataaa acttgctact acgcttccaa
     1141 tccagtaaat aaaaaaatag tacataactt tacctgtttt gttcggaagc taaattaata
     1201 cctcttgaaa ggaggatttt ttggctttcg atttcattac ttaaaagttc taactttcct
     1261 gagcgtatct tggatacaat tgcaggaatt cctccgacat aaagcatatt ggcctttaaa
     1321 attcgttcac cttttgaggt aaacgcgagt tcttgatgaa ttacacagag taaaccatcc
     1381 cctaaaatag aacgcaccaa ttcgatatca ccttcacttg ctgctatttg ttgtaaagca
     1441 taaatcgcac cttgtacagt gttaccatga atagtagaaa ctacaagatg tccgttaaca
     1501 gcagcacgaa tggcttcttt agcagtctta gcactacgta tttcaccaag aaaaatatac
     1561 ttaggattgt atcgcattgc agatatcatt gcattttcat agccacctgc ttgtcttgca
     1621 tctatctgat accaaatacc ctgagaatta tctccgtagc aaccctggac aggtatttca
     1681 ggtgggtctt ctattgcgat gactatatcg ccgaaagtag aaacatattc tttcagtaaa
     1741 gaatatattg ttgtcgattt acctgaccct gtagcacctg cgatcagaat catacctcgc
     1801 ttgtttccaa ggcattttaa agtatttaac attgcaggac tgaatcctaa acaatccagt
     1861 tcaggaatgg gtaattttaa acgtctcaaa aaatatccta cgccatcaac tgtttctaca
     1921 atggaggcac gaaaaggaat attctggtaa gaataaaaaa actcgtgctc accattatca
     1981 atacggatat taagttctgc cactattttc tttatttctg catgataaat ctcatcaaca
     2041 actgtgatag ggttacttgc acctttcagg taacgaaagt gatatccctt attactaatt
     2101 aataagtctg caaaataaat actttttaaa ctcataacta tcctagttaa actgagcagc
     2161 attgcttatt tgctgctgta gagagaacat accagaaaca atccacataa aaactccacc
     2221 cattatcagg attgctgcat tttttagaat attcatctga tttgaaataa attcaacgct
     2281 ctcttcctgc cattgcctag ataggcgttc cagcatcatt tcaaaaccat ctagtgctgc
     2341 gtatgaacgt atatccatca ccatattctt tgaaggaaag gaaaatccag atatatataa
     2401 agcttctcct atattaggag caccattcaa aagaatcaat ctaataaccg tcaatctctc
     2461 tctataccat ggagaagcat ttctgcttat tattcgtatc gcttccggcg taggaattcc
     2521 ggcagtgatt aatgatgaca acgataataa aaaaccacaa ccaataacag agcggtatat
     2581 tgaccatggt ggtattaggt cgaacttgct gcgtatttta cctgtccagt atggcagggt
     2641 gaaaattata atcactgtca gcattattaa aaaagcaatc aaatacatta aataaagctg
     2701 cacgaaaact gccagataat acatcaaacg tccagcccct tgccattctg taaatggcaa
     2761 aacctgttca aaggctggta caaccttagt tccaaaaata tataaaaaaa gacaagtact
     2821 aagtagcaat acaatagggt aaatgatacc aaagagtgcg gctctgactt ttttctttgt
     2881 tacatatata tatattatgt tacttattga atcactcaag ttacctgata tttctcctgc
     2941 cgcaagtata gataactcgt cctcaggaac ccaaccctta agagctttgg ttagcgaatc
     3001 accatttctg attgatatgg accacatatg aatagcaatg gcctggggtg ttttatttct
     3061 ctttccagaa tcagtagtga acttatataa ctcatctaaa gcaaaagtca ggggaacacc
     3121 gtttgctaga tacctggcta acttttcata aatacgcatt cttagttttg atgtaaaaag
     3181 cattttagca aaagctaact caaagttagg cattataatc gtcctctaaa aaacataact
     3241 ttctcaaata ttctcttcgc tcgttactta acgactctaa ttttgctatt ttatgtacag
     3301 cgtcaagaac atagatttcg ccagaaatga ttttcatcca tgcatgatct ctcatggtta
     3361 taccggataa acttgactgc cagtagttaa ctgcttcatt acgtttgcct tgatcaatta
     3421 aactaagaaa aatctgatca ggctcaatta tttctgcaat gatagttcta ccagagtatc
     3481 ctgattgaca gtgagagcat ccgtcattat ttggaaatct aatcgaatca tgatatccct
     3541 gaataatgtt ttcttctatt tcacttaaca catttgattg agtgaattcc tgatatgtca
     3601 gcgagcattg cggacatagt ttttttacaa gtctctgagc caccaaacca gtcactagtt
     3661 caggatcaat taatttaaaa ctatcaacac cctgatcttt taaacgatca aatatagaaa
     3721 cagcactatt tgcatgcaat gatgtccaaa cctgatgccc agtcatcgct gcagtaaaaa
     3781 ccaaattaat aacttctgcg tcccgagcct ctcctggcat tataatatct ggatcagagc
     3841 gtaatgcagc tgtaatagcc ttcctatatt ctgtagcacg ttcgtcttcg gtatcaacat
     3901 ttgttatagg taattgtgct gttccagtaa tttcatattc tggtgggtcc tcgatactaa
     3961 taatatttac tttagccttt ttttcaatat aaagtagttc aagaatgttt ttcaacgttg
     4021 ttgattttcc tgaaccggtt ggtccagaga taatattcac accaattggt aactgcctta
     4081 aacgtttgaa tgcttcagcc tgtacaggat ggaaaccata tttaacagga ttaagagcct
     4141 caatgctagt aatactcgtc ttctcaacat acagaaaacg agcaatcata tatcttccac
     4201 cttgccctaa agggttaaac tgaagacgaa tagattgtaa cctttccggt atagaaattc
     4261 tcgatttgga tgctataatc ctagcagctt gatacgaatt tagcttataa gttgagtcgg
     4321 catcctcagc tgtattgtaa agagcagcta ataggctatg acctttctct gcttcaattt
     4381 gtcgtaggag aaacatatct ccatcttttc ttatgaaaat ttcagcttca tcagtataaa
     4441 cttttatatg caaatcactt acccgaaggt cacaacactc atggattaga tctgaagcaa
     4501 tcttctccat tgggagtgat gaactttgaa ttactttctt ttcttgctct tgatataata
     4561 atcgaatatc tcttaatgaa actttatagc atgttatatt ttgtatacct ttacgtatag
     4621 ccacttcttt aagaaaacgg acggagggat tctcgagatg ctctgttgat ataagaaaat
     4681 ctccattttt ataaattata catatctttt tctgctcatc ggtcgtttca attaaccccc
     4741 ccttaacagt tacaatatca gatatattct tcctgaatct tagaaaatct tgatcatctg
     4801 cagtttttaa ctgttcaact atcatgccgc tatttctcat caaccaccac caattgtgta
     4861 aggctccaaa gtaagagttt tattttcttt agataaaaaa atagtaactt ttctttgatt
     4921 gatttctgtt acatgcatac tgtcatttat atcttcccct gttttaacta cacgcaagcc
     4981 tgctccacca ccatatactt ttgcggcata acctgatttt ccagaagaat atatagataa
     5041 aacagttaca ttttcaatac tattatcgcc taactttttc tttaactcct cattttgagc
     5101 ctttagtttt aactcgaagt tttctctctg gagctcctga aattttttta attgtgcttc
     5161 ataaccactt g
//
LOCUS       DAZGJT010000190         5086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZGJT010000190 DAZGJT010000000
VERSION     DAZGJT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.195"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..392)
                     /locus_tag="ACHKFK_004660"
                     /pseudo
     CDS             complement(24..392)
                     /locus_tag="ACHKFK_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908615.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            463..1008
                     /locus_tag="ACHKFK_004661"
     CDS             463..1008
                     /locus_tag="ACHKFK_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9054823.1"
                     /translation="MLIGYIRVSTNDQNTALQRNALECAGCELFFEDRISGKTRERPG
                     LKRALKRLRPGDTLLVWKLDRLGRSMQHLVLLTEELRQRGVNFRSITDNIDTSTPMGR
                     FFFHIMGALAEMERELIIERTRAGLAAARAQGRHGGRPQKLTEKEREQIARLLANGES
                     RKQIALIFDIGLSTLYRYFPA"
     gene            1122..3173
                     /locus_tag="ACHKFK_004662"
     CDS             1122..3173
                     /locus_tag="ACHKFK_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9054824.1"
                     /translation="MSDSKWKMLSAMPGEFSVKVAGGTVAFIESPFRPSGNKGGITFA
                     DCVIRFSTKEPLWVMPVSGNPSAEITSSGVTGIIPITSDVAGTLTPSDWNAPDNAGPS
                     GSTNGSSSEPEYYFVVILAGQSNGMAYGEGLPLPDSFDRPDPRIKQLARRSTVTPNGT
                     SCTYNDIIPADHCLHDVQDMSGINHPKADLAKGQYGTVGQGLHIAKKLLPYIPQNAGI
                     LLVPCCRGGSAFTTGADGSFSEASGASADSSRWGAGKPLYQDLVSRTKVALAKNPKNK
                     LLAVVWMQGEADLASGSQQHNSLFTAMVQQFRTDLAPLAAQCVSGNATTVPWICGDTT
                     YYWKNAGTDKYEAVYGGYKGKEAQKIFFVPFLTDENGQSTPTNAPAEDPDIVAVGYYG
                     AASRTQGSFVSTQRDSHFSSWARRGVISDRLASAIMLHAGRTAELMRGQTVTPPDEKP
                     SPDTPSKPSTPPADTTTMSTLFAYRASESEGRLTPQGWAAGGGKAQIVDDAGASGGKA
                     MKLTKETGRAPWYLEHDAGNGADLLGKGGLVSCRFKLDGALTANQYALALYWPVSALP
                     QGVTLEGNAGHNLLASFYVQSDATDLNVMYHKGNTAQNTKLGSFGAFNNEWHTLGFRF
                     AGNNSEVTPVIDGKDGTPFMLSQSPVGTFTADKLRVTDITKNATYPVLIDSIVVEVNN
                     A"
     gene            3318..3500
                     /locus_tag="ACHKFK_004663"
     CDS             3318..3500
                     /locus_tag="ACHKFK_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012908619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9054825.1"
                     /translation="MTFLQLILLYFCTAVCALYLVSGGYKAIRNYVRRKIEDAAAEKL
                     SKTAQATPLPKDPTQP"
     gene            3536..3790
                     /locus_tag="ACHKFK_004664"
     CDS             3536..3790
                     /locus_tag="ACHKFK_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9054826.1"
                     /translation="MPEIKGTVTEDLVKQALYSGEVNKLLKAQVRKDFEAQIDTYVDE
                     VLAKLIGPAAAASDADNEPKTDAQPEQPEPAQPGTDSTMM"
     gene            3866..4066
                     /locus_tag="ACHKFK_004665"
     CDS             3866..4066
                     /locus_tag="ACHKFK_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054827.1"
                     /translation="MDKNKKQPDTKGLERLSAEIINALRSGLDEDTLQDRNFATAMWI
                     AMQILCTPRCAIFAVREGQLTR"
     gene            4251..4439
                     /locus_tag="ACHKFK_004666"
     CDS             4251..4439
                     /locus_tag="ACHKFK_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Com family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9054828.1"
                     /translation="MYRNIRCRHCNKLLARASFSYLEVKCPRCKTLNQITSQSATEHP
                     TYTRKSCRGEQETLLSVR"
     gene            4405..4821
                     /locus_tag="ACHKFK_004667"
                     /pseudo
     CDS             4405..4821
                     /locus_tag="ACHKFK_004667"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
     gene            4835..4990
                     /locus_tag="ACHKFK_004668"
     CDS             4835..4990
                     /locus_tag="ACHKFK_004668"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054829.1"
                     /translation="MTMHSVIFLVRWPEQVAHYSQQGKDGQTLVLCLDHRVSSPGELN
                     RPGNPGD"
BASE COUNT         1355 a         1243 c         1306 g         1182 t
ORIGIN      
        1 aaaatatatt atttactgac tatttataac tgagataccg taatccatgt acctccgact
       61 aatttttgca ggggtctgaa tgctataaga tcgtttgaag catcaacttt actgtcaatc
      121 tgcaggcctg ccataacatt accgtacgca gagtaatatg ccccaccgta cattacctgc
      181 ctgttctctg ctcccagcct gatatcctgg atatatcggg aatccgcatc ctgctgtgtt
      241 ataccagaag tgatgtttat gtcctgcgtc ccgtcaaagg gaaccccatt aatatttctt
      301 ggggtggcga gtctattcgc ggcaacggcg gtgccgtccg atggcagagc attcgccgcc
      361 ctgtttaccg tttcccgtaa accgaggttt tcaggaaagc caggattaac tctgttttat
      421 tcctcatgcg acaattccgg ctttttataa taaggaaatc gtatgctgat tgggtatatc
      481 agggtgtcaa caaatgacca gaatacggcg ctacaaagaa atgcgctgga atgtgcagga
      541 tgtgagctgt ttttcgaaga cagaataagc ggtaagacaa gggagcgtcc cggacttaaa
      601 cgcgcattaa agcgactccg gccaggggat accctgctgg tctggaaact ggatcgcctt
      661 ggacgcagta tgcagcacct tgtcctttta acagaagaac tccggcaacg cggcgttaac
      721 ttccgcagca ttacggacaa tattgataca tccactccca tgggacgttt ctttttccat
      781 ataatgggcg cgctggcgga aatggagcgg gaactcatta ttgaacgtac acgcgcgggt
      841 cttgctgctg cccgtgctca ggggcgtcat ggcggcagac ctcagaaact gacagagaaa
      901 gagcgggagc aaatcgcccg tttgctggct aatggcgagt cccgtaaaca aatcgcactg
      961 atatttgata ttggcctttc caccctttat cgttattttc ctgcctaacc ggctccgtca
     1021 caaaaagtac cgtgatgctt tttttgaatt tccgcaaaac acatatttat cagcggttgt
     1081 tattctgact ctgtttaatt cagaaacagg cagaaaagac gatgtcagac tccaagtgga
     1141 aaatgctctc cgccatgccc ggagagtttt cagtcaaggt tgccggtggc acggtggcat
     1201 ttattgaaag ccctttccgc ccttcgggta ataaaggggg gatcaccttc gcagactgcg
     1261 tgatccgctt cagcacgaaa gagccattat gggtaatgcc ggtatccggt aatcccagtg
     1321 cagaaatcac cagttcaggc gtgactggca tcattccaat cacatctgac gtggccggaa
     1381 cgctcacgcc ctccgactgg aatgcaccgg ataacgccgg gccttccggt agcacgaatg
     1441 gcagcagttc agagccggaa tattactttg ttgtcattct cgcgggtcag tcaaatggca
     1501 tggcctacgg cgaggggctg ccgttaccgg attcgtttga ccgcccggac ccacgcatta
     1561 agcaactggc acgccgcagt acggtaacgc ccaacggtac gtcgtgtaca tacaacgaca
     1621 tcattccggc agaccactgc ctgcatgatg ttcaggatat gagcgggata aaccacccga
     1681 aagctgatct ggcaaaaggc cagtacggga ctgtcggtca ggggttgcat attgccaaaa
     1741 aactgcttcc ctatatccca cagaatgccg ggatacttct ggtgccctgt tgtcgtggcg
     1801 ggtcagcatt caccactgga gccgacggct cattcagtga ggccagcggt gcttccgctg
     1861 attcttcacg ctggggggcc ggtaaacctc tgtatcagga cctggtaagc cgcaccaaag
     1921 tcgcgctggc gaaaaacccg aaaaacaaac ttctggctgt ggtctggatg cagggagagg
     1981 cagaccttgc ctcaggaagc cagcagcata atagtttatt cacggccatg gttcagcagt
     2041 tcagaactga cctggctccg cttgctgcgc agtgcgtgag tggaaatgcc accacggtgc
     2101 cgtggatttg cggtgatacc acgtattact ggaaaaacgc tggcaccgat aaatatgagg
     2161 cagtatacgg tggctacaaa ggcaaggaag cacagaaaat tttctttgta ccgttcctga
     2221 cggatgagaa tggacagagc acgccaacga atgctccggc ggaagacccg gatattgtgg
     2281 ctgtcgggta ttacggtgca gcatcccgaa cccagggcag ttttgtttcg acacagcgtg
     2341 acagccattt cagctcatgg gcacgcaggg gcgtcatttc agaccgtctg gcctcagcta
     2401 ttatgctcca tgcagggcgc acggctgaac tgatgcgcgg gcagaccgtg acaccaccgg
     2461 atgagaagcc gtcacctgat acaccatcaa aaccgtccac gccacccgca gacaccacga
     2521 cgatgagtac gctatttgct taccgggcat ctgagtctga aggacggttg acaccacagg
     2581 gttgggctgc cggaggtggc aaggctcaaa tcgtggatga tgccggagcc agcgggggta
     2641 aggcaatgaa actgaccaag gaaacaggaa gagccccctg gtaccttgag catgatgctg
     2701 gtaatggtgc ggacctgctg ggtaaaggtg gtcttgtcag ttgtcgtttt aaactcgatg
     2761 gcgcgcttac ggctaatcag tacgcactgg cgctgtactg gccggtttct gccctgccgc
     2821 aaggggttac tctggaagga aatgccggtc ataacctgct ggcatcgttc tacgtacaga
     2881 gcgatgccac agaccttaac gtgatgtatc acaaaggaaa cacggcgcag aacacgaagc
     2941 tggggtcatt cggcgcattt aataacgaat ggcatacgct gggcttccgt tttgccggta
     3001 acaacagtga ggtgacgccg gtcattgatg gtaaggacgg gacaccattc atgctgtcgc
     3061 agtctccggt cggcacgttt acggcagaca agttgcgcgt gaccgatatc actaaaaatg
     3121 cgacttaccc ggtactgatt gacagtatcg tggtggaagt gaataacgcg taagcaggat
     3181 aaaaaaatgc cgccggtaag ggaaaacaag aaaccggcgg caaaaagatc gttatatcca
     3241 aagcaaaaaa tgcagctcac ctttttacct aacagatatt accggtgtca acaccatgtc
     3301 aataattggg agggataatg acatttttac agcttatttt attgtatttc tgcacggcag
     3361 tatgtgcgtt atatctggtg agtggtggtt ataaggccat cagaaattat gttcgccgca
     3421 aaattgagga cgcagcagct gaaaaactca gcaaaacagc gcaggcaact ccgctcccaa
     3481 aagacccgac ccagccctga ttgcagggct ttattcccgc ataaggagga tatacatgcc
     3541 agaaattaaa ggcaccgtca ctgaagacct ggtaaaacag gcactgtatt ccggtgaagt
     3601 aaataagttg ctgaaagcgc aagtccgcaa ggattttgag gcacaaatcg acacttacgt
     3661 tgatgaggtg ctggcaaaac ttattggacc agctgctgcg gccagtgatg ctgataatga
     3721 accgaaaacc gacgcgcagc cagagcaacc agaaccggct cagcccggaa ctgacagcac
     3781 catgatgtaa caatacaaac cggcagtttc tcctgtcggt tttattttca tgggcgcttt
     3841 atataccaat cagaggacaa taatcatgga caagaataaa aagcaacctg ataccaaagg
     3901 cctggaaaga ttaagcgcag aaattatcaa tgcgctacgt tcaggacttg atgaagatac
     3961 attacaggat cgaaacttcg ccaccgcaat gtggatagct atgcagatcc tgtgtacccc
     4021 aagatgcgct atttttgcgg ttcgcgaagg tcaactgact cgctgattgc acgaattaca
     4081 tcagaaaagc acgcgttcca taactgcata gcggatgaat cacctccctg aatcattgtt
     4141 gatgactttt caatctattt gttgtatcaa ttaaacaaat ctaatcgatc gtttttgtcg
     4201 atcgattagg cgagaaagat atcatcaaca aagcaggagg ttatgcggcc atgtatcgaa
     4261 acatacggtg ccgacactgc aataagctac tggcacgggc cagtttcagt tatctggaag
     4321 taaaatgtcc gcgctgtaaa accctcaatc aaatcacatc tcagagcgcc acagagcacc
     4381 ccacatacac aaggaaatcc tgccgtgggg aacaagaaac attactttca gtcagataag
     4441 tcactaacct atggttcagt atgcagtggt attgaagctg cgacagtagc ctggagcaat
     4501 cttggctgga aagccagatg gttcagtgaa atagaggact tcccgtcaac acttctttca
     4561 tatcgctggc cccatatacc aaatcttggt gatatggctt tgcttgcgcc tcgtatcagg
     4621 gatgggctta ttgaagcacc agatattctt gtaggtggaa ctccgtgtca ggcattcagt
     4681 atgtcaggca aacgaaaagg cctttcagat ccacgggggc agttaacact gtcttttgtt
     4741 gaactggcga atcagataga taccgttcgg gcatcgctca ataaatcccc ctcaataatc
     4801 gtatgggaaa acgtcacagg agttttaagt agccatgaca atgcattcgg tcattttctt
     4861 ggtgcgctgg ccggagcagg ttgcgcatta cagccagcaa ggaaaagatg ggcaaacgct
     4921 ggtgttgtgt ctggaccatc gcgtatcgtc gcctggcgag ttgaaccgcc ccggaaatcc
     4981 tggagactaa actccctgag aaagaggtaa acaggatgac taaaaatact cgtttttccc
     5041 ccgaagtccg tcagcgggcg attcgtatgg ttctggaaag tcagga
//
LOCUS       DAZGJT010000191         4947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZGJT010000191 DAZGJT010000000
VERSION     DAZGJT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.196"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..988
                     /locus_tag="ACHKFK_004669"
                     /pseudo
     CDS             1..988
                     /locus_tag="ACHKFK_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            1142..3139
                     /locus_tag="ACHKFK_004670"
     CDS             1142..3139
                     /locus_tag="ACHKFK_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFO9054830.1"
                     /translation="MSENDTIPKKSTSQINKAVFFTSALLIFLLVAFAAVFPDVADKN
                     FKLLQQQIFTNASWFYILAVALILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAML
                     FSAGMGIGLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVA
                     LILAFFSYRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQ
                     VNAGLNHLFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLAL
                     VLCLGPTVLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWS
                     PFVGMFIARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTV
                     QQDVALALFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVW
                     QRIFWASLMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKR
                     ESLSMATIAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQG
                     TISHISDAVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYR
                     AEVYLKEGGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            3202..4479
                     /locus_tag="ACHKFK_004671"
     CDS             3202..4479
                     /locus_tag="ACHKFK_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HFO9054831.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKK"
     gene            4725..4886
                     /locus_tag="ACHKFK_004672"
     CDS             4725..4886
                     /locus_tag="ACHKFK_004672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054832.1"
                     /translation="MTGENRGRGRPRKTESTYADTRNDLIRSGLELLTQNGFLATGVD
                     AIVSTTDEK"
BASE COUNT         1253 a         1141 c         1251 g         1302 t
ORIGIN      
        1 gttcccgcac ggtggctgaa ctggaatctg aaattctgca actgcgtaag gcgttaaatg
       61 aagctcgcct tgagcgagat atattaaaaa aagcaacgta ttttgcacag gagtcgctga
      121 aaaatacgcg ttaatcgaac aatggcgaca acaatttccc attgaagcga tgtgtcaggt
      181 atttggtgta tccaagagcg gttattacaa ctgggtacag catgaaccct cagacagaaa
      241 acaaagtgat gagcggctaa aactggagat taaggtggca catatccgca ctcgcgaaac
      301 atatggaacc cggcggctcc agacggagtt ggcagagaat ggcatcatcg ttggtcgtga
      361 ccgactggca cgtcttcgta aggagctaag gctacgctgt aagcagaaac gcaagttcag
      421 agcgactacg aactcgaacc acaatctgcc agttgcgcca aatctgctga accagacgtt
      481 cgctcctaca gcaccaaatc aggtctgggt ggcggacctg acgtatgttg ccacacagga
      541 gggatggttg tacctcactg gcatcaaaga tgtttatacg tgcgaaattg tcggctacgc
      601 catgggagag cgcatgacaa aagagctgac aggtaaagcc ctgtttatgg cgctcaggag
      661 ccagcgccca cctgccgggc taatccacca ctctgatcga ggttcacagt actgcgcata
      721 cgattaccgg gtcatacagg agcagtttgg tctgaaaaca tcaatgtcgc gtaaaggtaa
      781 ctgttacgac aacgctccga tggaaagctt ctggggaacg ctgaaaaatg agagcctgag
      841 ccactatcgt tttaataacc gggatgaagc catctcagta atacgggaat acattgagat
      901 tttctacaat cgtcagcgtc gtcactctcg tctggggaat atctccccgg cagccttcag
      961 ggaaaaatat catcagatgg ctgcttaaaa aaagaacaac tggtagtgtc cgctattgcc
     1021 agtacacctc aacattccac catgcattcc gattaacgcc gcatagccag ttgaactttg
     1081 ctactttgtg agaggtagta ccttctatcc agtgcgaatt taattaatgg aataaatgat
     1141 tatgagtgaa aatgatacaa tcccaaagaa gtctacaagt cagattaaca aagcggtatt
     1201 ctttacatct gctttgctaa ttttccttct tgtcgccttt gccgccgtat tcccggatgt
     1261 cgccgacaaa aattttaaac tacttcagca acaaatcttc acgaatgcca gctggttcta
     1321 catccttgct gtggccctga ttttactgag tgtcacgttc cttggactct cacgctacgg
     1381 tgatatcaag ctgggcccgg accatgcgca gcctgatttc agctaccact cctggtttgc
     1441 gatgcttttt tcggcaggga tggggatcgg cctgatgttc tttggcgttg ccgaacctgt
     1501 aatgcattat ctttcgccac ccgttggcac tccagaaacc gttgcggcag ctaaggaagc
     1561 aatgcgtctg acctttttcc actggggact gcacgcatgg gcaatttatg ccattgtggc
     1621 gctgattctg gcgttcttca gttaccgtca cggtctgcct ttaactctgc gctccgcact
     1681 ctatcccatt attggcgatc gcatatacgg acctgtagga catgcggttg atattttcgc
     1741 tgttataggc acggtctttg gcgttgcgac atcactgggt tacggtgttt tgcaggtgaa
     1801 tgccggtttg aaccatcttt tcggggtgcc catcaatgaa acggtgcagg taattctgat
     1861 cgtggtcatc acggggttag cgacgatttc agtggtgtcc ggtctggata agggaatacg
     1921 tatcctgtct gaactcaatc tgggtctggc tttgttgctc ctggcgctgg tcctgtgtct
     1981 gggaccaacc gtgcttctgc tgaagtcatt tgtggaaaat acgggcggtt atctttcgga
     2041 actggtgagt aaaacgttca acctttacgc gtatgagccc aagtcgagca actggctggg
     2101 gggctggaca ttactgtact ggggatggtg gctttcatgg tcgccgtttg tggggatgtt
     2161 catcgcacgg gtctcccgcg ggcgaaccat tcgcgagttt gtcaccggcg tgctgtttgt
     2221 tcccgcgggt tttacgctaa tgtggatgac ggtgtttggt aacagcgcga tctatctcat
     2281 tatgaaccag ggggccacag acctcgccaa tactgttcag caggatgtgg cgctggccct
     2341 gtttaatttc ctggagcatt tcccgttctc ttctgtgctg tcattcattg caatggcgat
     2401 ggtcatcgtc ttctttgtaa cgtctgctga ttcgggggca atggttgtgg atactctggc
     2461 atcaggtgga gtggcaaaca cacccgtctg gcagcgaata ttctgggcct cgctcatggg
     2521 cattgttgca attgcgcttc tccttgccgg agggctaagt gcgctgcaaa cggtgacaat
     2581 agcgagtgca ttgcccttct cagtgatctt actaatatct atatacggac ttttaaaagc
     2641 tttgcgccgg gatttgacca agcgtgaaag cctgagcatg gcgacaattg ctcctacggc
     2701 tgcacgtaac ccaattcctt ggcagagaag gttacgcaat atcgcgtatc tgccgaagcg
     2761 atctcttgtg aaacgtttta tggacgacgt tatccagccc gccatgacgc tggttcagga
     2821 ggaactgaac aagcagggga cgataagcca cattagtgat gcagtcgacg atcgtattcg
     2881 tcttgaagtc gatttgggca acgagctgaa ttttatatat gaagtgaggc ttcgcgggta
     2941 tatctcaccg accttcgcgc tcgccgcaat ggataatgat gagcagcaga ctgaacaaca
     3001 tcgatattat cgcgctgagg tttatctcaa agaaggcggt caaaattatg atgtgatggg
     3061 ctggaaccag gaacagctga ttaatgacat actggaccag tacgaaaaac acctgcactt
     3121 cctgcacctg gttcgttaat agcaacatgc cgtcctgggg gcggcaatta ttatcccggc
     3181 cgcaatatga gggaatgcag aatgatttca cgctggaaat ggatgctgaa gcagacaatt
     3241 aaaaaactat ggttcagggc aacgttattc gcaattgtcg cgataataac ggccctttta
     3301 tcaattcttt ttaaatcaat gatacctgag tcggtttccg tgaaggttgg tgcggaagca
     3361 gtcgataaca ttctgaacat actggcatcg agtatgctgg cagtgaccac attttcgctg
     3421 agtatcatgg tcacagccta cggttcagcc actactaatg tgactcccag agctacgcgt
     3481 ttagttgttg aagacgtcac cacacaaaat gtactggcca ccttcatcgg ttcttttctc
     3541 ttcagtctgg tagggattat tgccctcaat atgggagctt atggagaaag ggggagagtc
     3601 attttattca ttgtcacact ggttgtcatt gccttaatcc tcatcacatt gcttcgctgg
     3661 atacagcatt tgacctctct ggggagggtt ggtgagacaa cggcacaagt agaacaggcg
     3721 gccatcgaaa catttattgc gagagcaaga aatccctgtc tcggcggata tccatggctt
     3781 gagaacaatg aacagccgaa aggaacggtt gcagtttatc cgaagaagat tggctatgtt
     3841 gaatatattg atatggtgaa acttagcaag ctgctgacca atgatccccg tcatgtatac
     3901 ctcgtggcgc agccaggcag tttcatacat ccgtccatgc cagttttgta cctgagtcag
     3961 ggccaggagt cgtcaatcaa caccgattta cttgagacga ttattgtctc ggatgcacgt
     4021 tcatttgctc aggatcctcg attttgtctt agcgtcatgg ccgaaatagc ctgccgggcc
     4081 ctttcccctg cagtgaacga tcctggaacc gccattgatg tcattggcag aggtgttcgt
     4141 atactttcca cttacgcgca gaataaatct gatgaaatag aagtgaaata tccttcagta
     4201 catgttgcac cacttcagaa taacgatcta ctggaagact ttttctcacc tgtcgcgcgc
     4261 gatggtgcca gtatgaggga gattcagata agagtcctta aaggactgtc gatgctgagt
     4321 aaaggttggc ctgagatatt tgctgaagcc gcacataccc tggcatttga aacattagag
     4381 catgcaactc gtgctgacca tatagattct gatagatatc taataaaatc aacttattat
     4441 aatttattta gtggcgaata ttctaataaa aaaaaatagc tgcagagcaa gcaattcggt
     4501 tgttggcaag tcaagggcaa agcattcgcg tgagaatggg cgtatgcttt gtgccaaaag
     4561 caaacgttgc taattctgca ctgttcagcg atggcgcagt agcgacaggc actgccactg
     4621 tccagtcggc aaaagcgaac actttgccac tgcggggata aactgattga agctctcgcg
     4681 caaaatcact tattcttttg acaataaaat gtaggagatg ggtcatgaca ggcgaaaatc
     4741 gtggccgggg aaggccgcga aagacagaaa gtacctatgc agatactcgt aatgatctga
     4801 tccgcagtgg ccttgaactg ctgacgcaga atggttttct tgcgacggga gtcgatgcca
     4861 ttgtgtcaac gacggatgaa aagtgatcca cttatatctc caccaacggc ccaatattga
     4921 tccaccgttt tactcaggat tagcttc
//
LOCUS       DAZGJT010000192         4870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZGJT010000192 DAZGJT010000000
VERSION     DAZGJT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.197"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..1152
                     /gene="dhaK"
                     /locus_tag="ACHKFK_004673"
     CDS             88..1152
                     /gene="dhaK"
                     /locus_tag="ACHKFK_004673"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO9054833.1"
                     /translation="MKKLINQIDSVVTEQMEGLIATWPHLQANYAPRYVWCKQTDNAV
                     ALISGGGSGHEPLHAGFVGMGMLTGACPGEIFTSPTPDQMIECAKAVDNGSGVLFFIK
                     NYTGDILNFETAVEMLHEEGIAVGTVIIDDDVAVKDSLYTAGRRGVAGTVFVEKIVGA
                     AALQGYNLRQCEQLGKDVNNATRSFGIALSACTVPAAGKPSFELADNEIEFGVGIHGE
                     PGIERRTLQDLNTLIDSVIAQLLDNTPWRRTLRHWDRHAGGWIDASSMNESFDQNAEY
                     IVLINGLGSTPESELYGVARVFMCAAQRQGIKISRQLVGNYCTSLDMAGFSISLLKCT
                     PEFLQLWDAPVNTPALRWGC"
     gene            1165..1797
                     /gene="dhaL"
                     /locus_tag="ACHKFK_004674"
     CDS             1165..1797
                     /gene="dhaL"
                     /locus_tag="ACHKFK_004674"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO9054834.1"
                     /translation="MKIQNKHVIAWLESCAAHLTEQQDFLTALDRDIGDADHGLNMNR
                     GFSAVKATLPDIERQHIGNILKNTGMKLLSSVGGASGPLYGTLFIRASAAVGARTELS
                     LEEWLACLEEGIAGVIARGKAEQGDKTLCDVWVPVLHEAKKNLQAGMSPSLLLNTMVQ
                     DAATAVDNTINMQAKKGRASYLGARSVGHQDPGATSSWLMIKAMQEGFAG"
     gene            1801..4181
                     /gene="ptsP"
                     /locus_tag="ACHKFK_004675"
                     /pseudo
     CDS             1801..4181
                     /gene="ptsP"
                     /locus_tag="ACHKFK_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(4229..4612)
                     /gene="iraD"
                     /locus_tag="ACHKFK_004676"
     CDS             complement(4229..4612)
                     /gene="iraD"
                     /locus_tag="ACHKFK_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001695400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO9054835.1"
                     /translation="MMRQSVQTVLPESTGNNTLSLRDSVCRDLFQLFSSPHSPLPILL
                     VSGMPEWQGHNQSDKLLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHELAISLEMM
                     LYHDDEPLTFDLVWQKGSWHRTMPQ"
BASE COUNT         1265 a         1158 c         1233 g         1214 t
ORIGIN      
        1 cgtgcgattt gttttattgc ggctggagaa ttaatctcgc aagcatattt agcgcgattt
       61 acgtaattta tcagaacagg aatgactatg aagaaattaa ttaaccagat tgattctgtt
      121 gtcacagaac aaatggaagg actcatcgcc acctggccac acctgcaggc aaattacgca
      181 ccccgatatg tgtggtgcaa acaaaccgat aatgcagtgg cgctgatttc aggcggcggc
      241 tcaggccatg aaccactgca tgcaggtttt gtcggtatgg gaatgttaac cggtgcatgt
      301 cctggtgaaa tcttcacatc gcccacacca gaccaaatga tcgaatgtgc caaagcggtt
      361 gataatggtt ctggcgttct gtttttcatc aaaaattaca ctggcgatat tctaaatttc
      421 gaaacggcag tcgagatgtt acacgaagaa ggcatcgccg ttgggaccgt cattattgat
      481 gatgatgtag cggtaaaaga tagcctgtat acagccggac gtcgcggtgt tgcgggtaca
      541 gtttttgttg aaaagattgt cggtgcggct gcacttcagg gatataacct gaggcagtgc
      601 gagcaacttg gcaaagatgt taacaatgcc acccgttctt ttggcatcgc actgtcagcc
      661 tgtacggttc ccgccgcggg aaaaccctca tttgaactgg ctgataatga aatcgaattt
      721 ggtgtcggga tccacggtga acccggcatt gaacgccgca ctttgcaaga tctgaacact
      781 ttaatcgaca gcgtgatagc gcaattactg gataataccc cctggcgacg cacattacgc
      841 cattgggatc gccatgcggg gggatggatt gacgcatcat caatgaatga gtcattcgat
      901 caaaatgccg aatatattgt gttaattaac ggacttggca gcacgcctga atcagagtta
      961 tatggtgtag ccagggtatt tatgtgcgcc gcacagcgcc agggaattaa aattagccgc
     1021 cagttagtcg gtaattattg tacatcatta gatatggccg gattttccat tagcctgctg
     1081 aaatgtaccc cagagttttt gcaactttgg gatgccccgg tcaatacgcc agcgcttcgt
     1141 tggggatgtt aataaggaca ttatatgaaa attcaaaaca aacatgttat tgcctggctg
     1201 gagagttgtg ctgcacatct tactgaacaa caagattttc taaccgcact tgacagagat
     1261 attggtgatg ccgaccacgg cctcaatatg aatcgtggtt tcagtgccgt gaaagcgacg
     1321 ttgcccgaca ttgaacgtca gcatattggc aatatcctta aaaatactgg catgaaacta
     1381 ctttccagcg ttggcggtgc cagtggtcct ctttacggca cattatttat tcgcgcttct
     1441 gcggcagtgg gagccagaac agaattaagt ctggaagagt ggctggcgtg tctggaagaa
     1501 gggattgcag gcgttatcgc acgaggaaaa gcagaacaag gggataaaac actgtgtgat
     1561 gtctgggttc cagtcctcca cgaagccaaa aagaacctgc aagccggaat gtcgccttcc
     1621 cttctgctga atacgatggt gcaagatgcg gcaacagccg tcgataacac catcaatatg
     1681 caggcgaaaa aaggacgtgc cagttatctc ggcgctcgca gtgtgggcca tcaggatcca
     1741 ggcgcgacct cctcctggct gatgattaag gctatgcagg aaggattcgc gggataaatt
     1801 atggttgcca ttgttattgt ttctcatagt ctacgtctcg cccaggggat tgaggaatta
     1861 gcactacaga tgtctggagg ggacgttcct ctggccattg ctgcaggtat tgatgatccg
     1921 caaaatccga ttggcactga tgctattgcc attatgtctg cgattgaatc ggtctggtca
     1981 ccggatggcg ttctggtact catggatatg ggcagtgcgc tgcttagtac cgagatggcg
     2041 ctggagctgc tttctgaaga gcaaagaagt gcaatttatc tgctcgccgc ccctgtagtg
     2101 gaaggtgcca tgtcagcggt aacgtctgcc gcagcggggc tttcggtgac ggagattatc
     2161 gctgaagtgg atcttgcgct gtgcgctaaa cagcagcagc tcatgccatc cccgcctgca
     2221 ggcgaggtta ttccagcaat atcaccagtc aatcattccg gtgaatggca aactttttgc
     2281 tggacgatat gtaatcccca cggcattcat gctcgtccgg cagccagcat cctgaaagta
     2341 tcagcacaat attcggcaaa cattatcgtc atcaaaggcg ataaacgcgc cagcacccgc
     2401 agtctcaacg aactggcaat gctgggcgtc cgctgtggcg atgaaatcat cttccaggcc
     2461 gaaggcaccg atgccagtga cgctctcaag gcaattgaaa cgttggcgaa aaatcatttt
     2521 ggtgaagctc atctgctcgc ttcttcagag attccgctaa cggaaacgcc ggtggtcagc
     2581 gcccctgtcg atggcgcaat atcaggcttg tcagtacaaa atggtatcgc tattggtcct
     2641 gttaaatggt ttacctgcga acgaccagaa attacgcagc gcaccgtcga ttctccgcaa
     2701 gaagagctgt cgcgaatcga gtctgctatc gacattgttg tttgtgaact tgccgataaa
     2761 gcggcaggcc ctgaaggcga tattttgccg cccataaaat gatgcttgaa gatccagaga
     2821 ttaaccggca attacagcaa cgtctggcaa agggcaaaca ggcggaattc gcatggttag
     2881 aagtcatgca ggcattagca gagcaatatt gtcaggcgga aacactgtat ttacgtgaac
     2941 gcgaggcaga tatccgtgac cttacacgtc aggtactgaa ccagctttgc ggggtaagtg
     3001 aacaacactt tatcactacc gcgccctgca ttttgttagc caacgatctg ttgccatcac
     3061 agataaccag cctgaataaa gcacacattc ttggcatttg tcttcataat ggcggaacaa
     3121 cttcacatac agcgatcctt gccagagcaa tgggtattcc tgccattgtg aaggcagcta
     3181 ttaccccgca aaacgtccgc gataacgaca cggtcattct tgatggtgaa acaggccgtt
     3241 tgtggctaca gccggatgag gtcacccgcc tggatttact gcaacgcgcc gaagcatggc
     3301 ggcaacaaag ggatcgtcag ttagctgatg ccatgctgcc cgctgtcacg caaggtgggc
     3361 gtaaaatcag cgtgctggcg aatattggtg atctgcaaga cattgatgcc gcactgtctc
     3421 acggcgctga gggcgttggc ttgctgcgta ccgagttttt attccacgat tctgcgacat
     3481 tacctgatga ggaagagcag ttccgtgtgt attgttctgt cgcccgagtt tttggcgaca
     3541 aaccagtcac gatccggact ctggacattg gtggtgataa accgctacct tcataccctt
     3601 tacccacaga ggacaacccc ttcctgggac ttcgaggtat ccggttgtgt ctggcgcatc
     3661 cacaaatatt tattcctcaa ttgcgcgcac ttatcagggc cgggaaagaa tacccaacgt
     3721 tgcaaattat gctgccgatg gtttcaacgc tcgaggaggt cagggccgtc aaaacattga
     3781 tacatgctca ggcaaaactg ctgggactta ctgcagaaaa tcttccggcg ctgggaatta
     3841 tgattgaggt tcctgctgca gtcatgattg cagaaaaatt agccagtgaa gtcgatttct
     3901 tttccatcgg caccaacgat ctgacgcaat atattatggc ggcagacaga ggaaatagca
     3961 ccgtcgcgga actggtcgat tatcgtaatg acgcggtgat taacgctatt gctatggtgt
     4021 gtcaggccgg acgtaataat gaaattccgg tcagtatgtg cggcgaaatg gctggagata
     4081 tccagcaaac agcacgactg ctcaccatgg ggattgataa gctcagtgcc agtccatccc
     4141 ggcttccggc cttgaaagca gcaatcagaa cctcccacta attgtgacct aacgcgatac
     4201 gtcttgcgcg tatcgcgttt cccgtcattt actgcggcat cgtgcgatgc cagctcccct
     4261 tctgccagac caggtcaaac gttagcggct catcatcgtg gtacagcatc atctccagac
     4321 taatggccaa ctcgtggcaa taggcttctt tcagattgac ctgcaatgcc gcaatacgtg
     4381 gctcatgaaa taaaagcgcg gaacgtaact ggcggcaata ccaactctgg agaagcttgt
     4441 cgctctgatt atgcccttgc cactcaggca tgcctgaaac aagtaatatt ggtagcggtg
     4501 aatggggaga actaaaaagt tgaaagagat cccggcatac ggaatcacgc agcgataacg
     4561 tattgtttcc tgtactttca ggtaataccg tctgaactga ttggcgcatc attttgcgca
     4621 ctcctgacgt ttagtggcat ctgtatcctg ccctggctgc cggacaccac tgggttgatg
     4681 ctcatattcc ataatcattc ctttatgctc attgcgtccc tggcactttc ataagggatt
     4741 tataagatcc gccaaagata aaacactgta cagaaaagaa tatccactga cttttaattt
     4801 aaaaaggtat catttgacaa aagtcattaa acataacgga aatacgttaa catattctga
     4861 ttattccgaa
//
LOCUS       DAZGJT010000193         4851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZGJT010000193 DAZGJT010000000
VERSION     DAZGJT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.198"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(696..1004)
                     /locus_tag="ACHKFK_004677"
                     /pseudo
     CDS             complement(696..1004)
                     /locus_tag="ACHKFK_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1137..1292
                     /locus_tag="ACHKFK_004678"
                     /pseudo
     CDS             1137..1292
                     /locus_tag="ACHKFK_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1879..2016)
                     /locus_tag="ACHKFK_004679"
     CDS             complement(1879..2016)
                     /locus_tag="ACHKFK_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HFO9054836.1"
                     /translation="MRKRFFVGIFAINLLVGCQANYIRDVQGGTVAPSSSSKLTGISV
                     Q"
     gene            2064..2405
                     /locus_tag="ACHKFK_004680"
     CDS             2064..2405
                     /locus_tag="ACHKFK_004680"
                     /inference="COORDINATES: protein motif:HMM:NF015489.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO9054837.1"
                     /translation="MSLRYYIKNILFGLYCALIYIYLITKNNEGYYFLASDKMLYAIV
                     ISTILCPYSKYAIEHIFFKFIKKDFFRKRKNLNNAPVAKLNLFMLYNLLCLVLAIPFG
                     LLGLFISIKNN"
     gene            complement(2402..3967)
                     /locus_tag="ACHKFK_004681"
     CDS             complement(2402..3967)
                     /locus_tag="ACHKFK_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin-like pore-forming protein"
                     /protein_id="HFO9054838.1"
                     /translation="METAVAYYKDGVPYDDKGQVIITLLNGNPDGSGSGGGGGTGGSK
                     SESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQHLKDIVNEAL
                     RHNSTHPEVIDLAHANNAAMQAEAERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIE
                     KEQAETERQLKLAEDEEKRLAALSEEARAVEVAQKNLAAAQSELAKVDEEINTLNTRL
                     SSSIHARDAETNTLSGKRNELDQASAKYKELDERVKLLSPRANDPLQSRPFFEATRLR
                     ARAGDEMEEKQKQVTASETRLNQISSEINGIQEAISQANNKRSTAVSRIHDAEDNLKT
                     AQTNLLNSQIKDAVDATVSFYQTLSEKYGEKYSKMAQELADKSKGKKISNVNEALAAF
                     EKYKDVLNKKFSKADRDAIFNALEAVKYEDWAKHLDQFAKYLKITGHVSFGYDVVSDI
                     LKIKDTGDWKPLFLTLEKKAVDAGVSYVVVLLFSVLAGTTLGIWGIAIVTGILCAFID
                     KNKLNTINEVLGI"
     gene            4227..4664
                     /locus_tag="ACHKFK_004682"
     CDS             4227..4664
                     /locus_tag="ACHKFK_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HFO9054839.1"
                     /translation="MGHCNFMAWHTLRDALKMTGRSRSQFYRDMRSGLVSYRTSQDGR
                     REFETSELIRAYGELKRNETPERHTQGHSEKPHDQQTERILRELNELKQCLTLMLEDK
                     QAQDTERRRQEAERKQLQNEIAQLRQALEQEKKRGFWSRLFGH"
     gene            4745..>4851
                     /locus_tag="ACHKFK_004683"
     CDS             4745..>4851
                     /locus_tag="ACHKFK_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 2"
                     /protein_id="HFO9054840.1"
                     /translation="MRRTGNKLCLIAMITATVALTACTPKGSVEQHTRH"
BASE COUNT         1310 a         1252 c          988 g         1301 t
ORIGIN      
        1 attcactgcc tgtcctgtgg acagacagat atgcacctcc caccgcaagc ggcgggcccc
       61 taccggagcc gctttagtta caacactcag acacaaccac cggaaaaacc ccggtccagc
      121 gcagaactga aaccacaaag cccctccctc ataactgaaa agcggccccg ccccggcccg
      181 aagggccgga acagagtcgc ttttaattat gaatgttgta actacattgt catcgctgcc
      241 agtcttctgg ctggacgtac tgaatacacg ctcgtaagcg gccctgacgg cccgctaacg
      301 cggagatacg ccccgacttc gggtaaaccc tagtcgggac cactccgacc gcgcacagaa
      361 gctctttcat ggctgaaagc aggtatggct taacagggct ggggatgggt aaggtgaaat
      421 ctatcaatca gtaccggctt acgccgggct tcggcggttt tactccggta tcacatgtaa
      481 ccacagcgtg ccgccttaca tgccgctggc gcggcatatc ctggacgtat aggaaattga
      541 aaaaacagtc gataccattg tccacgcctt atgccacgtg agcctttctg cttttcccac
      601 cactccggtg gtttctctct ctgtatggtc gatttttata ccgtgacagg ctgtttttta
      661 tacttgcacc gattcccggg cacacctagc ctgtgtcagg ccggcatata cctcagcaga
      721 ctgatatccc gcgcgttttt cttcagccct gatacattca gccccgcaat ggcccggcgg
      781 tataccatcc ggccgccgca cagcacgcat tcgaacgggt cacgactcag gaactgtttc
      841 accatttgtg catagcacac tttcgccact gcttccggtt tatccatccc cagagcacgg
      901 tacacctgcg gcagcttctc tccacacacc cggttggcaa ggaacccgaa gtacctcacc
      961 atcttaaaaa acttctccgg gatgtgctgt ttcagcctcg cgacccgttc ttccgttgta
     1021 atgcgccagc cgggaagccg ctattgggta agcgtaaact gaccgccgta tgtagccatc
     1081 agacgaaaat tggtaactta gacgcccatc tgatatagac ggacatctaa gtatggaatt
     1141 acaggactgg cgaaaagaac ctcgttaaaa actattcgaa tgaattcaaa cttcgtatgg
     1201 tggaactgtc atcacaacct ggagcttgtg ttgcacagat tgcacgtgaa aatggcgtca
     1261 atgataatgt tattatcaaa tggctcaggc tctgacagaa cgaaaggcgt gtttcgcggc
     1321 gtcttccggt aacgacctct tctgacactg gcgttgaatt attacctgta gaaataacgc
     1381 cggatgcgta gtactcacca ataaataagc gtcaatacgg atacctgtcc gcctttctcc
     1441 cttcgggaag cgtggcgctt tctcatagct cacgctgttg gtatctcggc tcggtgtagg
     1501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     1561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttagcg ccactggcag
     1621 cagccgttgg taacttggtt agcagagcga tgatggcgca aatggtgcaa cagagttctt
     1681 gaagtagtgg cccgactacg gctacactgg aaggacagta tttggtatct gcgctctgct
     1741 gaagccagtt acctcggtta agcagttccc caactgactt aaccttcgat caaaccgcct
     1801 ccccaggcgg ttttttcgtt tacaggcagc agattacgcg cagaaaaaaa ggatctcaag
     1861 aagatccttt gatcttttct actgaacaga gatccccgtc aatttagaag aagatgatgg
     1921 agccacagtc cctccctgaa catcacgtat atagttagcc tgacatccaa caaggaggtt
     1981 tatagcgaat attcccacaa aaaatctttt cctcataact cgatccttat aaaatgaaaa
     2041 gaatatataa cgaggtttaa tttatgagtt taagatacta cataaaaaat attttgtttg
     2101 gcctatactg cgcacttata tatatatacc ttataacaaa aaacaacgaa gggtattatt
     2161 tcctagcgtc agataagatg ctatacgcaa tagtgataag cactattcta tgcccatatt
     2221 caaaatatgc tattgaacac atatttttta agttcataaa gaaagatttt ttcagaaaaa
     2281 gaaaaaacct aaataatgcc cccgtggcaa aattaaacct atttatgcta tacaatctac
     2341 tttgtttggt cctagcaatc ccatttggat tgctaggact gtttatatca ataaagaata
     2401 attaaatccc caacacctca tttatagtat taagtttatt cttatcaata aaggcacata
     2461 gaatgcctgt aacaatagca atcccccaga tacctaatgt agttccagca agcacactaa
     2521 aaagtaaaac aacaacataa ctaactccag catctacagc tttcttctct aatgtaagaa
     2581 atagcggctt ccagtcacct gtatccttaa tttttaggat atcagatacc acatcatatc
     2641 caaaagaaac atgtcccgta atcttcaagt acttggcaaa ctgatctaaa tgcttagccc
     2701 agtcttcata cttaaccgcc tccagtgcat tgaaaattgc atcacggtct gctttgctga
     2761 atttcttatt taatacatcc ttgtatttct caaaagcagc tagagcttca ttcacattgc
     2821 tgattttctt acctttagat ttatcagcaa gttcctgtgc catttttgaa tatttttcac
     2881 catatttttc agatagcgtt tgataaaagc taactgttgc atccacagca tccttaatct
     2941 gcgagttcag gagattagtc tgtgctgttt tcaaattatc ttcagcatca tgaatacgtg
     3001 aaactgctgt acttcgctta ttattagcct gagaaatagc ctcttggatt ccatttatct
     3061 cagagctaat ctggttaaga cgcgtttctg atgctgttac ctgcttttgt ttttcctcca
     3121 tctcatcacc agctctcgcc cgtagtctgg tggcctcaaa aaaaggacgg ctctgaagcg
     3181 gatcattcgc cctaggagat aaaagtttaa ccctttcatc cagttcttta tatttagcag
     3241 atgcctgatc caactcattt cgttttccgg acagcgtatt cgtttctgca tcacgggcat
     3301 gaatactgga gcttaaacgg gtattgagcg tattaatctc ttcatccact ttcgccagct
     3361 cagattgtgc agcagcaaga tttttttgtg ccacctccac agcccgggcc tcttcactca
     3421 atgctgccag gcgtttctct tcatcctcag ccagtttcaa ttggcgttct gtttcagcct
     3481 gctccttctc tatctcttta cgtcgttgtt ctgcttcctg aaaagccttt tccgcagctt
     3541 ccgcttcttt acgggctttt tcttctgctt ttgcaaggcg caaccgctct gcttctgcct
     3601 gcatcgctgc attattggca tgagccaggt caataacctc cggatgagtg gaattatggc
     3661 gaagcgcctc attcacaata tccttcagat gttgagtcag cgcatcccgg tttgcttttg
     3721 ctttagcctg tgcttctgcg gcagcttttg ctcgggcagc ctgttctgcc tgcgttttct
     3781 tcaattgagc agtagaccat ttagctgtgg catgaatggc tgcagaactt tcacttttgc
     3841 tacctccagt tccaccacca ccgccagagc cactcccgtc tggattacca ttcaaaagag
     3901 taatgattac ctgcccctta tcatcataag gaacaccatc tttatagtac gctacagctg
     3961 tttccattat aaaatcctct ttgacattaa aaacaaatag ttaaaagcaa tatactgtat
     4021 atataatcac tggttttata tacagtttaa aattacgccg ctgcattttc cctgtcaacc
     4081 ctgtggattt tcatttttgt gaaaacgatc aaaaaaacag ccacccacac cacgtgctat
     4141 ttccacccga tgccacaaaa accagcacaa acattaccgt tctcagacct cattatgttt
     4201 tactgaaacc atgagatgag acacctatgg gacactgtaa ctttatggca tggcacacac
     4261 tccgggacgc actgaaaatg actggaagat cgcgttcaca gttttaccgt gatatgcgct
     4321 caggtcttgt cagttaccgt accagccagg acggacgacg ggagtttgaa accagtgaac
     4381 tgatccgggc atacggcgaa ttaaagcgga atgagacacc agaaaggcac actcaaggac
     4441 actcagaaaa acctcatgat cagcagacag aacgcattct ccgggaactg aatgagctga
     4501 aacaatgcct gacgctgatg cttgaggata aacaagccca ggatacggag cgccggcgcc
     4561 aggaagcaga acggaaacag ctacagaatg agatagccca gctcaggcag gctctggagc
     4621 aggaaaagaa acggggattc tggtccaggc tgttcggtca ctgaacgcgc tcagaaactg
     4681 atgataagat aatcttcgga taataactca ccgagaataa atactttaag gtagggagac
     4741 actcatgaga cgtaccggaa acaaactttg tcttatcgcc atgataacag caacagtagc
     4801 tctcacagcc tgtaccccaa agggcagcgt ggaacaacat acccggcatt a
//
LOCUS       DAZGJT010000194         4790 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZGJT010000194 DAZGJT010000000
VERSION     DAZGJT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.199"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..1100
                     /gene="hemB"
                     /locus_tag="ACHKFK_004684"
     CDS             126..1100
                     /gene="hemB"
                     /locus_tag="ACHKFK_004684"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO9054841.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1140..1991)
                     /gene="tauD"
                     /locus_tag="ACHKFK_004685"
     CDS             complement(1140..1991)
                     /gene="tauD"
                     /locus_tag="ACHKFK_004685"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO9054842.1"
                     /translation="MSERLSITPLGPYIGAQITGADLTRPLSDNQFEQLYHAVLRHHV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIQTPPAGAILAAKELPSTGGDTLWASGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEAEHQRWREAVAKNPPLLHPVVRTHPVTGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     IPFYRAG"
     gene            complement(1988..2815)
                     /gene="tauC"
                     /locus_tag="ACHKFK_004686"
     CDS             complement(1988..2815)
                     /gene="tauC"
                     /locus_tag="ACHKFK_004686"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO9054843.1"
                     /translation="MSVLINEKLHSHRLKWRWPLSRQVTLSIGTLAILFTVWWVVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVLIGIPVG
                     IAMGLSPTVRGILDPIIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2812..3579)
                     /gene="tauB"
                     /locus_tag="ACHKFK_004687"
     CDS             complement(2812..3579)
                     /gene="tauB"
                     /locus_tag="ACHKFK_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO9054844.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKGIQGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGVEK
                     MQRLEIAHQMVKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQIQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSPGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3592..4554)
                     /gene="tauA"
                     /locus_tag="ACHKFK_004688"
     CDS             complement(3592..4554)
                     /gene="tauA"
                     /locus_tag="ACHKFK_004688"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO9054845.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVVYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVATSQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1111 a         1376 c         1221 g         1082 t
ORIGIN      
        1 attacaaaga tgtcctgacc tctttttcat tctctttttg tcaacatcgc gacaactttc
       61 gtaaaacaca cccagcctgc ttcaggtata ctatgctcct cgattccaca aacatcaggc
      121 agaccatgac agatttaatc caacgccctc gtcgcctgcg caaatctcct gcgctgcgcg
      181 ctatgtttga agagacaaca cttagcctta acgacctggt gttgccgatc tttgttgaag
      241 aagaaattga cgactacaaa gccgttgaag ccatgccagg cgtgatgcgc attccagaga
      301 aacatctggc acgcgaaatt gaacgcatcg ccaacgccgg tattcgttcc gtgatgactt
      361 tcggcatctc tcaccatacc gatgaaaccg gcagcgatgc ctggcgggaa gatggactgg
      421 tggcgcgtat gtcgcgcatc tgcaagcaga ccgtgccaga aatgatcgtt atgtcagaca
      481 cctgcttctg tgaatacaca tctcacggtc actgcggtgt tctgtgcgag catggcgtcg
      541 acaacgacgc gactctggaa aatttaggca agcaagctgt ggttgcagct gctgcaggcg
      601 cagacttcat cgccccttct gccgcgatgg atggtcaggt acaggcgatt cgccaggcgc
      661 tggacgctgc gggctttaaa gatacggcga ttatgtcgta ttcgaccaag ttcgcctcct
      721 ccttttatgg cccgttccgt gaagctgccg gaagcgcatt aaaaggcgac cgcaaaagct
      781 atcagatgaa cccaatgaac cgtcgtgagg cgattcgtga gtcgctgctg gatgaagccc
      841 agggcgcaga ctgcctgatg gttaaacctg ccggagcata cctcgacatc gtgcgtgagc
      901 tgcgtgaacg tactgaattg ccgattggcg cgtatcaggt gagcggtgag tacgcgatga
      961 ttaagttcgc cgcgctggcg ggtgctatag atgaagagaa agtcgtgctc gaaagcttag
     1021 gttcgattaa gcgtgcgggt gcggatctga ttttcagcta ctttgcgctg gatttggctg
     1081 agaagaagat tctgcgttaa tgatcattat gccgggttat ccaacccggc atttccccct
     1141 taccccgccc gataaaacgg aatatcccca agaatcgtcg cccgatgcat aatccgccgc
     1201 tgtggcaggt aatccgcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata
     1261 tcattcggtt gccagcgcca gcgcacctga aactccggtt tagtgatatg ggcaaacaaa
     1321 aaacctaaca aggcttcgct ctctttctcg ctgacatcaa caattcgtgt ggtaaaacct
     1381 tcgttcacaa acagcgcctg tttaccggtc accggatgcg ttcgcaccac cggatgtagc
     1441 aacggtggat ttttcgcgac cgcctcacgc cagcgttgat gctcagcctc ggttttgcgg
     1501 tacttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcaaccc gctcaacagc
     1561 tggcggaagg gaacagagag cgcctcatat gcggcaatac cactggccca gagcgtatcg
     1621 ccgccggtcg agggtaactc tttcgccgcc agaatcgccc ctgcgggtgg cgtttgaata
     1681 aatgtcacat cggtgtgcca gttgtcgtta tccggcggat tatcgttatg ggtatccagg
     1741 acgatgatct cgtcaacccc ttcagcatga gggtaaacag ggtgaatatg cagctcgcca
     1801 aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatag cttgatcgcg cagaaacacc
     1861 acatgatggc gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc
     1921 gttaagtcgg cccccgtaat ttgtgcgcca atatacggcc ccagcggggt aatgctcaga
     1981 cgttcactca ttgtacttct ccatgccagg gcgtcaggcg gcgctgtaac gcgcgcagac
     2041 ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat ccccgccagc accacgtcag
     2101 tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgcgtt gcggcaatca
     2161 gctccgccgc caccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga
     2221 tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca
     2281 gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc agcgccgaca
     2341 tcgccaccgg cgcaaaaatc gctagataga tcagtaagat cttcgaggtt tcgccaatac
     2401 caaaccagat caccatcagc ggcaaataag ccagcggcgg caccggacga taaagctcga
     2461 ttatcggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa
     2521 taccgatcaa caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg gctgccagat
     2581 gctgccacag cgtggcgtcc ataaatccct gcggcccggc aatggtgagc agttttgcca
     2641 gtacctgttg cggtggcggc aaaaacagcg ggctaatcag ttgcagcgcc gcgactaccc
     2701 accatacagt gaataaaatc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg
     2761 gccagcgcca tttaagccga tgcgaatgca gtttttcatt aatgagcaca ctcatgagaa
     2821 cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg
     2881 atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgggcga agttgagcgg
     2941 cagccgctcc agcacgcggc ctgggccggg tgaaagcaga accagttcag tcgccataaa
     3001 caccgcttct tctatatcgt gggtaatcaa cagcacctgc tttcccgtct cctgccagag
     3061 tttcagcagc agggtttgta tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc
     3121 gtcgagtaat aacagctggg gatttgccgc caacgcgcgg gcaatcccca cccgctgacg
     3181 ttgaccaccg gaaagctgcc agatgtagcg tttttctgcg ccttccagcc cgactttttt
     3241 caccatctgg tgtgcgattt ccagtcgctg cattttctct acacctgcca gttgcaggcc
     3301 gaacgccacg ttgtcctgta cattgcgcca cggtaacaac ccttcattct gaaaaactac
     3361 gccacgctct gctcccggtc cctgaatacc cttacccgcc agttgaatgc tgccatgctg
     3421 ataaggcaca aaaccagcaa tcaaattcag caacgtagtt ttgccgcagc cagacggccc
     3481 cagaactacc agaagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt
     3541 gccgccataa tcggcgtaaa gatgagaaat ttgcagcatc tgcgcctcct tttattgcac
     3601 gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggactt tgccctgctc
     3661 tttcaaaaac tgcgcggtgt cgatgatcgc tttgttcacc ggtccggtca gttctgccgt
     3721 ttgttgctgc ggcgtcagat aggtatttcc cttcaccagc cccggaacgt caccttccgg
     3781 cacgccgctt aaccgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtctgg
     3841 gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt tcacgacctc
     3901 aggatgtttc tcggcaaaat ctttccgcac gacccagacg tccagtgtcg gcgcgcccca
     3961 ctgcccgacc tgttcagaat cggtcagcac cttgccatct ttttccaggg cgttaaccgc
     4021 cggtgcccag acataagcac catcaatatc tccccgctgc catgcggcga taattgcggg
     4081 cggctgcagg ttcacaatct caacttgccc aggtttaata ccccagtgtt tcagcgccgc
     4141 cagcaggctg tagtgggtgg tggagataaa cggtacggcg atgcgtttac caatcaagtc
     4201 ttccggttta ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc
     4261 cagcaagaag acttcaatcg gcacctgttg gctggttgca accgctaacg ggctggaacc
     4321 gaggttgccg atttgcacat cgcccgaagc cagcgcccgc acgatgctgg ctccactgtc
     4381 aaacttacgc cagtccacgg ttgctccgct ttctttagca aaggtgttgt cggcctgagc
     4441 cactttcgcc ggttcggcgg aggtttgata caccacggtg acgttcaccg cctgcgcctg
     4501 aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg ccatgattgt
     4561 ctgctccccc gtcttgttat gggagcagta ttcaggaata aaaacattca ttaaaagaat
     4621 tagtcgttat cgtacagatg attttattct tagcaaaaaa gcggcaaagg aaagattaaa
     4681 tatgcaatta cgcccactct gataactcag taaattactg cacttcattt tattaatgca
     4741 taaacaagca ggttctaatg tctgttattt tattaacagc aacacatata
//
LOCUS       DAZGJT010000195         4764 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZGJT010000195 DAZGJT010000000
VERSION     DAZGJT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4764
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.200"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..784
                     /locus_tag="ACHKFK_004689"
     CDS             <1..784
                     /locus_tag="ACHKFK_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003834018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO9054846.1"
                     /translation="HELATETRKDYRKYGSKVNVVFGKMKPDNIKPEHIRKYMDKRGV
                     KSRVQANREKAFMSRVFRWAYERGKVKMNPCQGVKQFKEQARTRYISDREYDALYSVA
                     SIPVKVAMELAYLCCARQGDILDLKKSQLLSEGILIQQSKTAVSQIKAWTKRLEDAVN
                     LADSIPLNSGMVSIFVIHQPSGLRYTRDAFNAQWMKTKKLAAEKYPDLDFQFTFHDLK
                     AKGISDLEGTLSEKQEISGHKNASQTARYNRRISVVPVVGGQ"
     gene            complement(910..996)
                     /locus_tag="ACHKFK_004690"
     tRNA            complement(910..996)
                     /locus_tag="ACHKFK_004690"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(960..962),aa:Leu,seq:taa)
     gene            complement(1009..1082)
                     /locus_tag="ACHKFK_004691"
     tRNA            complement(1009..1082)
                     /locus_tag="ACHKFK_004691"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1048..1050),aa:Cys,seq:gca)
     gene            complement(1136..1211)
                     /locus_tag="ACHKFK_004692"
     tRNA            complement(1136..1211)
                     /locus_tag="ACHKFK_004692"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1176..1178),aa:Gly,seq:gcc)
     gene            complement(1363..1911)
                     /gene="pgsA"
                     /locus_tag="ACHKFK_004693"
     CDS             complement(1363..1911)
                     /gene="pgsA"
                     /locus_tag="ACHKFK_004693"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO9054847.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(1968..3800)
                     /gene="uvrC"
                     /locus_tag="ACHKFK_004694"
     CDS             complement(1968..3800)
                     /gene="uvrC"
                     /locus_tag="ACHKFK_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO9054848.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGIGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3797..4453)
                     /gene="uvrY"
                     /locus_tag="ACHKFK_004695"
     CDS             complement(3797..4453)
                     /gene="uvrY"
                     /locus_tag="ACHKFK_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO9054849.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     ANAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
BASE COUNT         1266 a         1203 c         1118 g         1177 t
ORIGIN      
        1 ccacgagtta gcaactgaaa cacgtaaaga ctacagaaaa tacggttcaa aggtaaatgt
       61 tgtcttcggg aaaatgaagc cggataacat caagccagag cacatcagaa aatatatgga
      121 taaacgaggt gttaagagca gggttcaggc aaaccgcgag aaagcattta tgtccagggt
      181 gtttcggtgg gcatacgaac gaggaaaggt gaagatgaat ccttgccagg gcgtaaagca
      241 atttaaagag caggctcgga cccgttatat tagcgatagg gaatatgatg cactttatag
      301 cgttgcttct attcccgtaa aagttgcaat ggagttggca tatctatgtt gcgcccgcca
      361 gggagacatt cttgacctca aaaagagtca attattgagc gaaggaattt taattcagca
      421 gagtaaaacc gcagttagcc agatcaaggc ctggacaaaa cgtctggagg atgccgttaa
      481 tcttgctgac agcattcctc ttaacagcgg gatggtaagt atttttgtca tccaccagcc
      541 atcaggtctg cgatacacac gcgatgcttt taatgcgcaa tggatgaaga ctaaaaaact
      601 tgctgctgaa aaatatccag accttgattt ccagttcact tttcatgatc taaaagccaa
      661 aggaatttct gatcttgaag gtacgttaag tgagaagcag gaaatttcag gtcataaaaa
      721 cgcctcgcaa acagcgagat ataatcgaag aatttcagtt gttccggtcg tcggggggca
      781 gtaatgccct ctttttatgg cgaagctgaa tggcgaaaca atggtgaaca tggagtacaa
      841 aaacaaaaaa accacccgta ggtggtttca cgacactgct tattgctttg attattctgt
      901 tctttcccat ggtacccgga gtgggacttg aacccacaca gcgcgaacgc cgagggattt
      961 taaatccctt gtgtctaccg attccaccat ccgggctcgg gaagaaagtg gaggcgcgtt
     1021 ccggagtcga accggactag acggatttgc aatccgctac ataaccgctt tgttaacgcg
     1081 ccaaattctt caggcctttc agccagacat ccgctgccgc cgatgtcttt taaactggag
     1141 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
     1201 gctattcccg cattcatcaa gcaatcagtt aatcacttga ttttattatc gtctggcaat
     1261 cagtgccgcc gttcgatgcg ttgcattcta cttacctggc gcgatgagtc aacgatattt
     1321 ttcaccactt ttgatcgttt gctgaaaatt gcgccgaaac gatcactgat caagcaaatc
     1381 tgcacgcgca gcgctcaaat attgcaacat tgaccacaga gtcagtaccg cagccacaaa
     1441 gaaaagtgca ataccggcgt actcaaccca aatgttcgga cgccacagca gccaggctaa
     1501 cgccaccatc tgggcagtgg ttttcacttt cccaatccag gagacagcca cgctactgcg
     1561 tttacccaac tccgccatcc attcgcgtag cgcagaaata ataatttcac gggcgatcat
     1621 cgttgctgcc ggtaaggtca cccaccagct gtggtaatgc tcggttacca gcaccatggc
     1681 gatagccacg agaactttat ctgcgacagg gtcaaggaaa gcaccaaacc gggtactctg
     1741 attccagcgg cgtgccagaa aaccatcgaa ccagtcagtc accgccgcga cgcagaaaat
     1801 gagcgcggcg gcaaacggcg accaggtgac aggcagataa aagaccaata caaagaatgg
     1861 gataaggatg acacggaaca gtgtaagcaa cgtagggata ttaaattgca taatgacggg
     1921 taactatctg ttgtcagtaa gattacccct atgttgctac agagacatca atgtttcaac
     1981 gaccagaaga tcttttctgc cagaccttgc gaaatacccg gcacttttgc aatttcctca
     2041 acgctggcgt tacgtaaacc ttgcaaacca cccatatatt tcaacaacat ttgccgacgt
     2101 tttggcccga tgccttcaat ggtttccagg gaactggtat ttttgacctt cgcccgtttt
     2161 ttacggtgcc cgccaatcgc gtgatcgtgt gattcatcgc gaatatgctg gataacatgc
     2221 agcgcgggag aatctggcgg caaactaaat ccctcacctt ccggctcaaa gaacagcgtt
     2281 tccagcccag ccttacgatc tgctcctttg gcaacgccaa gtagcagcgg atgattttta
     2341 tcccatgaga catccagttc ggcgaagaca tttttcgcct gcgcaagctg gcctttgccg
     2401 ccgtcgataa ggatcacatc cgggatctta ctgtcgtcaa tggctttacc ataacgccga
     2461 cgcagcacct gattcatcgc cgcataatca tcgcccggtg tgatgccagt aatgttatag
     2521 cgccgatact ccgcacgcag cgggccgtta gcatcaaaca ccacacagga agcgacggtt
     2581 tgttcaccca tggtatggct gatgtcaaag cactccatcc gcttcacttc cggcaatttc
     2641 aacacgctgg caagcgcggt cagccgctgg tgaacggtag attgctgcga aagtttgctg
     2701 gttaaggccg tcgccgcatt ggtacgcgcg agtttcagat aacgcgctct atcgccacga
     2761 ggtttggttt gaacattaat cttgcgtccc gccagttctg aaagggaatc ggcgagcagc
     2821 gttttatcgc taagattaaa atcgagcagg atctcacccg gtaaggtgcg catctggctg
     2881 ccttgtaaat agaactgacc tacgaaggtt tccaccacct cgctcagttc cgtaccgcca
     2941 ggcactttcg ggaaatagct gcggctgccg agcactttgc cctgacgaat gaacaatacg
     3001 tggacacaag ccatacccgc atcgaacgcc actccaataa cgtcaaggtc gtcgccggta
     3061 ttggaaacaa actgtttttc ggtgacacgt cgcaccgcct gaatttggtc gcgaatccgc
     3121 gccgcttctt caaactccag attctggctg gcagtttcca tacgactaat gagttgcgta
     3181 agcacctgat catctttgcc agacaaaaac aggcgcacat actcgacctg ctgagcgtat
     3241 tcttcttcac tcaccagtcc ttcaacgcac ggccccagac agcgccctat ctgatattgc
     3301 agacacggac gcgagcggtt gcgataaaca ctgttttcgc actggcgaat ggggaaaatc
     3361 ttttgcagta gcgccagtgt ttcacgtacg gcatagccat tcgggaacgg gccgaaatat
     3421 tcacctttgg catgcttcgc tccacgatgc atcgccagac gcgggtgggt atcgccactc
     3481 aggaagataa aaggatatga tttatcatcg cgtagcaaaa cgttgtaacg cggctgataa
     3541 agtttgatgt agttgtgttc cagtaacagc gcttcggttt ctgtatgagt aaccgttaca
     3601 tcaatttgct ggatctgggc gaccagcgct tcggttttgc gcgaagcgag gttgctacgg
     3661 aaatagctgg aaagccgttt tttcaggtct ttcgctttgc cgacatagat aaccgtacca
     3721 ccagcatcgt acatgcgata aacgcctggc tggctggtta cggtttttaa aaacgctttt
     3781 gcgtcaaact gatcactcac tgacttgata atgtctccgc attacacaga ccatggcgaa
     3841 ttgccaggtg agtcagctca acatcgccat gaatgtttag tttactgaac atacgatagc
     3901 ggtagctgtt caccgttttc ggactgagat tgagctgttc tgagatctca ttgaccttct
     3961 ggcccttggt gatcatcagc ataatctgca attcacgttc agacaaactg gcaaatgggc
     4021 tttctgtttt ttctggttcg atctggctta acgccatttg ttgagcgata tcagaagcaa
     4081 tgtaacgctg ccctgaatag acagaacgaa tcgcactcac gacttcctgc ggagccgcgc
     4141 ctttgctgag gtagcccgca gctccggcct gcatgacttt cgctggtaaa gggttttctg
     4201 tatggacggt aagcatgatg attttgacat cagctgtgga acgcgcgatt ttacgcgtcg
     4261 cctcaagacc gccaatgccc ggcatactca tgtccattag caccacgtca acggcatttg
     4321 cccggcacca tttaacggcg tcttcaccgc acgatgcctc accgacgact tttataccct
     4381 ttatatcttc cagaatgcgt cgtatccctg cgcgcaccag ttcgtggtca tcaacaagta
     4441 gaacgttgat caaaggaata tctccagaaa tagggataac gctactgata gttagtcatt
     4501 cgtatattag cggtttttat tgcaactttg aaacgttaaa aatggtgggt attcgatttt
     4561 tctctcgttt ttgtcaattc gattgtccac agttatggtc acgcccgtca ccaacggctt
     4621 ccgggatcgt ttcacagtaa gcccggcgat gaaatgtgac aaaaattgac tttattcagc
     4681 aaaaatgaaa atcagcccgc tgaatatgta acattaatta acccgaacaa tacgggcaaa
     4741 aaacattcat gataaaaata ttta
//
LOCUS       DAZGJT010000196         4643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZGJT010000196 DAZGJT010000000
VERSION     DAZGJT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.201"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1289
                     /gene="cadA"
                     /locus_tag="ACHKFK_004696"
     CDS             <1..1289
                     /gene="cadA"
                     /locus_tag="ACHKFK_004696"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO9054850.1"
                     /translation="RVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDS
                     AWVPYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETF
                     NEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESD
                     GWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMS
                     DFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLN
                     LRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPY
                     AAFQKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQML
                     CEIGAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            1348..2805
                     /gene="dtpC"
                     /locus_tag="ACHKFK_004697"
     CDS             1348..2805
                     /gene="dtpC"
                     /locus_tag="ACHKFK_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO9054851.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSCHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSALRVWLKFAFGLLLMACGFMLLAFDARHAAAGGQASMDVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            3042..4559
                     /gene="lysS"
                     /locus_tag="ACHKFK_004698"
     CDS             3042..4559
                     /gene="lysS"
                     /locus_tag="ACHKFK_004698"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO9054852.1"
                     /translation="MSERETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPLK"
BASE COUNT         1156 a         1078 c         1160 g         1249 t
ORIGIN      
        1 agcgcgtgaa agaaacacca aacgcaacct ggccggtaca tgctgtaatt accaactcta
       61 cctatgatgg tctgctgtac aacaccgact tcatcaagaa aacactggat gtgaaatcca
      121 tccactttga ctccgcgtgg gtgccttaca ccaacttctc accgatttac gaaggtaaat
      181 gcggtatgag cggtggccgt gtagaaggga aagtgattta cgaaacccag tctactcaca
      241 aactgctggc ggcgttctct caggcttcca tgatccacgt taaaggtgac gtaaacgaag
      301 aaacctttaa cgaagcctac atgatgcaca ccaccacctc tccgcactac ggtatcgtgg
      361 cgtccactga aaccgctgcg gcgatgatga aaggcaatgc aggtaaacgt ctgatcaatg
      421 gttccattga acgtgcgatc aaattccgta aagagatcaa acgtctgaga acggaatctg
      481 atggctggtt ctttgatgta tggcagccgg atcatatcga tacgactgaa tgctggccgc
      541 tgcgttctga cagcacctgg cacggcttca aaaacatcga taacgagcac atgtatcttg
      601 acccgatcaa agtcaccctg ctgactccgg ggatggaaaa agacggcacc atgagcgact
      661 ttggtattcc ggccagcatc gtggcgaaat acctcgacga acatggcatc gttgttgaga
      721 aaaccggtcc gtataacctg ctgttcctgt tcagcatcgg tatcgataag accaaagcac
      781 tgagcctgct gcgtgctctg actgacttca aacgtgcgtt cgatctgaac ctgcgtgtga
      841 aaaacatgct gccgtctctg tatcgtgaag atcctgaatt ctatgaaaac atgcgtattc
      901 aggaactggc tcagaatatc cacaaactga ttgttcacca caatctgccg gatctgatgt
      961 atcgcgcatt tgaagttctg ccgactatgg taatgactcc gtatgctgcg ttccagaaag
     1021 agctgcacgg tatgaccgaa gaagtttacc tcgacgaaat ggtaggtcgt attaacgcca
     1081 atatgatcct tccgtatccg ccgggagttc ctctggtaat gccgggtgaa atgatcaccg
     1141 aagaaagccg tccggttctg gagttcctgc agatgctgtg tgaaatcggc gctcactatc
     1201 cgggctttga aaccgatatt cacggtgcat accgtcaggc tgatggccgc tataccgtta
     1261 aggtattgaa agaagaaagc aaaaaataat tagctcgtac aagggaagtg gcttgccact
     1321 tccctttttt gctcataagg agaacacatg aaaacaccct cacagccgcg cgcgatatac
     1381 tatatcgtgg cgatccaaat ctgggagtac ttcagttttt acggcatgcg tgccttactc
     1441 attctctatc tcacccatca gcttggtttt gatgataacc atgccatcag cctgttcagc
     1501 gcatatgctt ctctggttta tgttacccct attctcggcg gctggcttgc cgaccgcctg
     1561 ctcggcaacc gcactgcagt gattgccggc gcgctgttaa tgacccttgg ccatgtggtg
     1621 ctgggcattg atacaaattc aacctttagc ctgtatctgg cgctggcaat cattatttgt
     1681 ggctacggtt tattcaaatc aaacatcagc tgtttgcttg gcgagctcta cgacgagaac
     1741 gatcatcgac gtgatggcgg tttttcgctg ctgtatgctg cgggcaatat cggttctatc
     1801 gccgcgccca tcgcctgcgg cctggctgct cagtggtatg gatggcatgt tggctttgcc
     1861 cttgcgggag gcggcatgtt tatcggtttg ttgattttct taagttgtca tcgtcatttc
     1921 cagtccacgc gtagtatgga taaaaaagcg ctcacaagcg tcaaatttgc cttaccagta
     1981 tggagctggt tagtggtgat gctctgttta gccccagtat tttttaccct gctgctggag
     2041 aacgactggt ccggatattt gctggcgatc gtttgcctca ttgccgcaca aatcattgcc
     2101 cgcatgatga tcaaattccc cgaacaccgc cgtgctcttt ggcaaattgt attgttgatg
     2161 tttgtcggga cgttgttctg ggtactggca caacagggcg gcagtaccat cagcttgttt
     2221 atcgatcgct ttgtgaatcg tcaggcattc aatattgaag tacctacagc actattccag
     2281 tcggtgaatg ctattgcggt gatgctcgct ggggttgttc tggcctggct ggcgtcgcca
     2341 gaaagccgcg gcaattcagc attgcgcgtc tggctgaagt ttgcctttgg cttactgctg
     2401 atggcttgtg gctttatgtt gctggcattt gatgcccgac atgcagcggc tggcggtcaa
     2461 gcgtcaatgg acgtgatgat atccgggctg gcgctaatgg gctttgctga actctttatt
     2521 gacccggtgg cgattgcgca aatcacgcgt ctgaaaatgt ctggcgtatt aactggtatt
     2581 tatatgctgg caacaggcgc agtcgccaac tggctggcag gcgtcgtggc acagcagacg
     2641 acagagtcgc aaattagcgg tatggcaatt gccgcttacc agcgattctt ttctcagatg
     2701 ggagagtgga cgttggcttg tgtcgcgatc atcgtggtat tggcctttgc tacccgtttt
     2761 ctgtttagca cgccgacgaa tatgatacag gagagcaacg attaactgtc gtaaaaacaa
     2821 ttggttatga ataaattaac tttcagttta tcacactagc aaatcgagat taataagtca
     2881 tttaaaccat ttttatggtt atttattagt gatatcaact tgaggtaaac gttagtttcg
     2941 ataagataaa ctgagttatt aatagtcgag gcagataata cagtgtaccg atctgtctct
     3001 ttatctacgc taaattgaaa gctggattta gaggaaccaa aatgtctgaa cgagaaacac
     3061 ggggagccaa tgaggctatt gattttaacg atgaactgag aaatcgccgc gaaaaactgg
     3121 cggcactacg tcagcaaggt gtggcgtttc ccaatgattt tcgccgcgac catacctctg
     3181 accagttgca cgaagagttt gatgcgaagg ataaccagga actggaatcc ttaaacattg
     3241 aagtctcggt tgctggccga atgatgaccc gtcgtattat ggggaaagcc tcctttgtaa
     3301 cgttgcagga tgtcggtggc cgtattcaac tgtacgttgc aagagatagc ctgccagaag
     3361 gtgtttataa cgatcagttt aaaaaatggg atctgggtga cattatcggt gcccgcggta
     3421 cgctgtttaa gacgcaaacg ggtgagcttt ccattcactg tactgagctg cgcctgctga
     3481 ctaaagcact acgtccttta ccagataaat tccatggtct gcaggatcag gaagtccgtt
     3541 atcgtcaacg ttatctggac ctcatcgcta acgataaatc ccgtcaaacg tttgttgtcc
     3601 gttcaaaaat tctggccgct atccgtcagt tcatggtcgc acgcggcttt atggaagtag
     3661 aaaccccgat gatgcaggta attccaggtg gggcatctgc tcgcccgttt attacccatc
     3721 ataacgctct ggatttagat atgtacctgc gtatcgcgcc ggagctgtat ctgaaacgtc
     3781 tggttgtagg cggttttgaa cgggtattcg aaatcaaccg taacttccgt aatgaaggta
     3841 tttctgttcg ccataatcct gagttcacaa tgatggaact ctacatggca tatgcggatt
     3901 accatgattt gattgaactg acggagtcac tgttccgcac cctggcacaa gaggtactgg
     3961 gtaccactaa agtcacttat ggcgagcatg tgtttgattt cggcaaaccg tttgaaaaac
     4021 tcaccatgcg cgaagcaatc aaaaaatatc gtccagaaac cgatatggcg gatctggata
     4081 attttgatgc tgctaaagca ttagctgaat ctatcggtat tacggtagag aaaagctggg
     4141 gactgggacg tattgttacg gagatctttg atgaagtggc agaagcacat ctgattcagc
     4201 caacctttat tacggaatat ccggcagaag tgtccccgct ggcgcgccgt aatgatgtta
     4261 acccggaaat caccgaccgt tttgaattct tcatcggtgg tcgtgaaatt ggtaatggtt
     4321 ttagcgaatt aaacgacgca gaagatcagg ctgaacgttt ccaggaacag gttaacgcta
     4381 aagctgcagg tgacgacgaa gccatgttct atgacgaaga ttacgtgact gcgctggaat
     4441 atggtctgcc gccaaccgct ggtctgggta ttggtatcga ccgaatgatt atgctgttta
     4501 caaacagtca tactattcgc gacgttattc tcttcccggc gatgcgccca ctgaaataaa
     4561 tttcacttta atgaacgaag cagtcaggcg actgcttcgt tcgtcattcc gcaaatccag
     4621 ttttgaggag agacgcttcg ttt
//
LOCUS       DAZGJT010000197         4557 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZGJT010000197 DAZGJT010000000
VERSION     DAZGJT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.202"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..848
                     /gene="yibB"
                     /locus_tag="ACHKFK_004699"
     CDS             <1..848
                     /gene="yibB"
                     /locus_tag="ACHKFK_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO9054853.1"
                     /translation="TTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALEN
                     EMIIFTSPDLKSRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPRQ
                     LKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWDF
                     PFDENKMHLFTIKKWLAVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLESQ
                     KITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALRI
                     FLSRK"
     gene            1123..2319
                     /gene="kbl"
                     /locus_tag="ACHKFK_004700"
     CDS             1123..2319
                     /gene="kbl"
                     /locus_tag="ACHKFK_004700"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO9054854.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLSAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            2329..3354
                     /gene="tdh"
                     /locus_tag="ACHKFK_004701"
     CDS             2329..3354
                     /gene="tdh"
                     /locus_tag="ACHKFK_004701"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO9054855.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            3593..>4557
                     /gene="waaH"
                     /locus_tag="ACHKFK_004702"
     CDS             3593..>4557
                     /gene="waaH"
                     /locus_tag="ACHKFK_004702"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO9054856.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPTDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIRFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFR"
BASE COUNT         1171 a          971 c         1163 g         1252 t
ORIGIN      
        1 ccaccacaat tatcactgca tattttgaca ttggtagagg ggattggact gctaataaag
       61 ggttccgtga aaaactagct cgttcagttg atgtttattt tagttacttt gagcgtctgg
      121 ccgcacttga aaatgaaatg attattttca cttctcccga tttgaaatca agggttgaag
      181 cgattcgcaa cggaaaacca acaacggtta tcgttatcga tataaaaaag aaatttagat
      241 atatcagaag tcgtatcgaa aaaattcaaa aagatgagtc gtttacaaat agacttgagc
      301 cgcggcaatt aaaaaaccca gagtactggt caccagagta tgtattggta tgtaacctta
      361 aggcctattt tgtaaataaa gctatcaaca tgggcttagt gaaaactcct cttgttgcgt
      421 ggatagattt tggctattgc cgtaagccaa atgtaactcg aggattgaaa atttgggatt
      481 tcccatttga tgaaaataag atgcatcttt tcactataaa aaaatggctt gctgttacat
      541 ctcaacaaca ggcttttgat ttcatgattg gcaatcatgt atatattatt ggtggcgcaa
      601 ttgttggttc acagcataaa tggaaagaat tctataaatt agttctggaa agtcaaaaaa
      661 taaccctaaa caataacatt gtggacgatg accagggcat ttttgtcatg tgctattaca
      721 aacgttccga tctcttcaac ctaaactatc tggggagagg aaaatggttc gatttgtttc
      781 gctgcttcag gagtaacact ttaggggcaa aaatgcaggc gctgagaatt ttcctttcaa
      841 gaaaataggt ttccctccat tagtgggtag cgttttgata ccaaatgaga ttggaaaaat
      901 gatgctctgg catcattttt tctttttata tcaatgcagt aaaatcactc atcattatgt
      961 tcagccaaaa ctgtacagtt tttctactat ctgtaggtgt taagagacat caagatacac
     1021 gttatttatc ctgaattttg cagaagtgtt aacgcgtcat ctcgtcgcga cctataagtt
     1081 tgggtaatat gtgctggaat ttgccctgtc tggagaatcg caatgcgtgg agaattttat
     1141 cagcagttaa ccaacgatct ggaaaccgca cgggcggaag ggttgtttaa agaagagcgc
     1201 attattacgt ctgcgcagca agcagatatc actgtggctg atggaagcca cgtcattaac
     1261 ttttgtgcca acaactatct cggactggcg aatcatccgg atctgattgc ggcggcaaag
     1321 gcgggaatgg attctcacgg tttcggcatg gcttcggtgc gttttatttg cggcactcag
     1381 gacagccata aagagcttga acaaaaactg gcggcattcc tggggatgga agatgcgatt
     1441 ctctactctt cctgctttga tgctaacggc ggcctgtttg aaacgctgct gagcgcggag
     1501 gacgccatta tctccgacgc actgaaccac gcgtctatta ttgatggtgt gcgtctgtgt
     1561 aaagctaaac gctatcgtta tgccaacaac gatatgcagg agctggaagc acgtctgaaa
     1621 gaagcgcgtg aagccggtgc gcgtcatgtg ctgattgcca ccgatggtgt gttctcgatg
     1681 gacggcgtga ttgccaacct gaagggcgtt tgcgatctgg cagataaata cgatgccctg
     1741 gtgatggtag acgactccca cgcggtcggt tttgtcggtg aaaatggtcg tggttcccat
     1801 gaatactgcg atgtgatggg gcgtgtagac atcattaccg gtacgttggg taaagcattg
     1861 ggcggggctt ctggtggtta taccgcggcg cgcaaagaag tggttgagtg gctgcgccag
     1921 cgttctcgtc cgtacctgtt ctccaactcg ctggcaccgg cgattgtcgc cgcatccatc
     1981 aaagtgctgg agatggtcga agcgggtagc gagctgcgtg accgtctgtg ggcgaacgcg
     2041 cgtcagttcc gtgagcaaat gtcggcggcg ggttttaccc tggcgggagc cgatcacgcc
     2101 attattccgg tcatgcttgg cgatgcggta gtggcgcaga aatttgctcg tgagctgcaa
     2161 aaagagggca tttacgtcac cggtttcttc tatccggtcg ttcctaaagg tcaggcgcgt
     2221 attcgtaccc agatgtctgc ggcgcatacc cctgagcaaa ttacgcgtgc agtagaagca
     2281 ttcacgcgta ttggtaaaca actgggcgtt atcgcctgag ggtgtgagat gaaagcgtta
     2341 tccaaactga aagcggaaga gggcatctgg atgaccgacg ttcctgtacc ggaactcggg
     2401 cataacgatc tgctgattaa aatccgtaaa acagccatct gcgggactga cgttcacatc
     2461 tataactggg atgagtggtc gcaaaaaacc atcccggtgc cgatggtcgt gggccatgaa
     2521 tatgtcggtg aagtggtagg tattggtcag gaagtgaaag gcttcaagat tggcgatcgc
     2581 gtttctggcg aaggccatat cacctgcggt cactgtcgca actgtcgtgg cggtcgtacc
     2641 catctgtgcc gcaacacgat cggcgtcggt gttaaccgtc cgggctgctt cgccgagtat
     2701 ctggtgattc cggcgttcaa tgccttcaaa atcccggaca atatctccga cgacctggct
     2761 tccatttttg atcccttcgg taacgctgtg cataccgcgc tgtcgttcga tctggttggc
     2821 gaggatgtgc tggtctctgg tgcaggtccg ataggtatta tggctgcggc ggtggcgaaa
     2881 cacgttggtg cacgcaatgt ggtgatcact gatgttaacg aataccgcct tgagctggca
     2941 cgcaaaatgg gtatcacccg tgcggttaac gtcgctaaag aaaaccttaa tgatgtgatg
     3001 gcagaactgg gcatgaccga aggctttgat gtcggtctgg aaatgtccgg tgcgccgcca
     3061 gcgtttcgta ccatgctcga caccatgaac cacggcggtc gtattgcgat gctgggtatt
     3121 ccaccgtccg atatgtctat cgactggacc aaagtaatct ttaaaggctt gttcattaaa
     3181 ggtatttacg gtcgtgagat gtttgaaacc tggtacaaga tggcggcgct gattcagtct
     3241 ggcctcgatc tctcgccgat cattacccat cgtttctcta tcgatgattt ccagaagggc
     3301 tttgatgcca tgcgttcggg ccagtccggg aaagttattc tgagctggga ttaacacgaa
     3361 caagggctgg tattccagcc cttttgcctg aggataatcc gttaaatatg taaaatcctg
     3421 tcagtgtaat caagatatcg taattgcact gatctcttat atagctgctc tcattatctc
     3481 tctaccctga cgtgactctc tcaccggtaa aaataatatc tcacaggctt aatagtttct
     3541 taatacaaag cctgtaaaac gtcaggataa cttcagaggt cgtcggtaat ttatgatgaa
     3601 cagcaccaat aaacttagtg ttattattcc gttatataat gcgggcgatg atttccgcac
     3661 ttgtatggaa tctttaatta cgcaaacatg gactgctctg gaaatcatta ttattaacga
     3721 tggttcaacg gataattctg ttgaaatagc aaagcattac gcagaaaact atccgcacgt
     3781 tcgtttgttg catcaggcga atgctggcgc atcggtggcg cgtaatcgtg ggattgaagt
     3841 ggcaacggga aaatatgtcg cttttgtcga tgctgacgat gaggtctatc ccaccatgta
     3901 cgaaacgctg atgaccatgg cgttagagga cgacctcgac gtggcgcagt gcaacgctga
     3961 ttggtgtttt cgtgaaacgg gtgaaacctg gcaatccatc cctaccgatc gccttcgctc
     4021 aaccggtgta ttaactggtc cggactggct gcggatggga ctttcttccc gccgttggac
     4081 acacgtggtc tggatggggg tttatcgccg cgatgttatt gttaaaaata acattagatt
     4141 tattgccgga ttacatcatc aggatattgt ctggacaaca gaatttatgt ttaacgcgct
     4201 gcgtgcgcga tataccgagc aatcattata taaatattat ctgcataata cgtcagtgag
     4261 tcggttgcat agacaaggga ataaaaacct taattatcaa cgtcactata ttaagattac
     4321 ccgcctgctg gagaaattaa atcgaaatta tgccgacaaa attacgattt atccggaatt
     4381 tcatcagcaa ataacttacg aagcattgcg tgtttgccat gcggtgcgca aagagccgga
     4441 tattattacc cgccaacgga tgattgccga gatatttact tccggtatgt ataagcgcct
     4501 gattactaat gtgcgcagcg tgaaggtggg ttaccaggcg ttactgtggt ctttccg
//
LOCUS       DAZGJT010000198         4444 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZGJT010000198 DAZGJT010000000
VERSION     DAZGJT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.203"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..434)
                     /locus_tag="ACHKFK_004703"
     CDS             complement(<1..434)
                     /locus_tag="ACHKFK_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO9054857.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDTPTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVAATDGPMPQTREHILLGRQVGVPYIIVFLNKCDMVDDE"
     gene            complement(549..624)
                     /locus_tag="ACHKFK_004704"
     tRNA            complement(549..624)
                     /locus_tag="ACHKFK_004704"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(589..591),aa:Thr,seq:ggt)
     gene            complement(631..705)
                     /locus_tag="ACHKFK_004705"
     tRNA            complement(631..705)
                     /locus_tag="ACHKFK_004705"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(670..672),aa:Gly,seq:tcc)
     gene            complement(822..906)
                     /locus_tag="ACHKFK_004706"
     tRNA            complement(822..906)
                     /locus_tag="ACHKFK_004706"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(870..872),aa:Tyr,seq:gta)
     gene            complement(915..990)
                     /locus_tag="ACHKFK_004707"
     tRNA            complement(915..990)
                     /locus_tag="ACHKFK_004707"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(955..957),aa:Thr,seq:tgt)
     gene            1352..2302
                     /gene="coaA"
                     /locus_tag="ACHKFK_004708"
     CDS             1352..2302
                     /gene="coaA"
                     /locus_tag="ACHKFK_004708"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO9054858.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2331..3296)
                     /gene="birA"
                     /locus_tag="ACHKFK_004709"
     CDS             complement(2331..3296)
                     /gene="birA"
                     /locus_tag="ACHKFK_004709"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO9054859.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEKILSQLDDGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQHAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLTPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3293..4321)
                     /gene="murB"
                     /locus_tag="ACHKFK_004710"
     CDS             complement(3293..4321)
                     /gene="murB"
                     /locus_tag="ACHKFK_004710"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO9054860.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFDAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETANALLAQFPTAPHYPQVDGSVKLAAGWLIDQCQLKG
                     TQIGGAAVHRQQALVLINEHDAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1141 a         1112 c         1009 g         1182 t
ORIGIN      
        1 tcttcgtcat caaccatgtc gcatttgttc aggaacacga tgatgtacgg aacgcctacc
       61 tgacgcccca gcaggatgtg ctcacgagtc tgcggcatcg ggccgtcagt cgcagcaact
      121 accaggatcg cgccgtccat ctgagcagca ccggtgatca tgtttttaac atagtcggcg
      181 tgccccgggc agtctacgtg cgcgtagtga cgggtcgggg tgtcgtattc aacgtgagaa
      241 gtgttgatgg tgataccacg agctttttct tccggcgcgt tatcgatctg gtcgaatgcg
      301 cgagcagcac cgccgtaggt tttagccagt acggtagtga ttgcagcggt cagcgttgtt
      361 ttaccatggt caacgtggcc gatagtaccg acgttaacgt gcggttttgt acgttcaaac
      421 ttttctttag acatcgattg tccctctaag acacggataa atcggtgata tcaccacatc
      481 aaccaggcaa catgcccgac ttgttgaatg caataaacag aaggaaaaaa cagggaggag
      541 aaaagaagtg gtgctgatag gcagattcga actgccgacc tcacccttac caagggtgcg
      601 ctctaccaac tgagctatat cagcacatct tggagcgggc agcgggaatc gaacccgcat
      661 catcagcttg gaaggctgag gtaatagcca ttatacgatg cccgcatcct ggaactcggc
      721 tacctgattt tcattctgca ctgaatatcg agagaagctc tctttattcg agccggtaag
      781 cgaacttatc gtctcgggct acgccatcgc gtggccgaaa ttggtggtgg gggaaggatt
      841 cgaaccttcg aagtctgtga cggcagattt acagtctgct ccctttggcc gctcgggaac
      901 cccaccggac ttgatggtgc cgactaccgg aatcgaactg gtgacctact gattacaagt
      961 cagttgctct acctactgag ctaagtcggc atcaagtagc gcgcattcta tggagacatg
     1021 cgagttcatg caactaaaaa attgcataat ttgttttatt ggtcacattt tacgcgacac
     1081 gatgaagaaa cagccgaacg gagggcagat tagcacactt tttcaacatc attgtgctca
     1141 acaatgtgct cctgctaaac cataattctt tttatcagat ggaatatctg tcacattgct
     1201 tttcaacgat agcttcctgg gagagatttt ttcttattat tcctccccat ctggtgttac
     1261 cctcctgccc attaacccat tcaacagaac tgtgacgcgc catggcaaat atcgctttgc
     1321 cgatagagct atgaccgcca gaaacatgct tatgagtata aaagagcaaa cgttaatgac
     1381 gccttaccta cagtttgacc gcaaccagtg ggcagctctg cgtgattccg taccgatgac
     1441 gttatcggaa gatgagatcg cccgtctcaa aggtattaat gaagatctct cgttagaaga
     1501 agttgccgag atctatttac ctctgtcacg tttgctgaac ttctatataa gctcgaatct
     1561 gcgccgtcag gcagttctgg aacagtttct tggtaccaac gggcaacgca ttccttacat
     1621 tatcagtatt gctggtagtg tcgcggtggg gaaaagtaca accgcccgtg tattgcaggc
     1681 gctattaagc cgttggccgg aacatcgtcg tgttgaactg atcactacag atggcttcct
     1741 tcaccctaat caggttctga aagaacgtgg tctgatgaag aagaaaggct tcccggaatc
     1801 gtatgatatg catcgcctgg tgaagtttgt ttccgatctc aaatccggcg tgccaaacgt
     1861 tacagcccct gtttactcgc atcttattta tgatgtgatc ccggatggag ataaaactgt
     1921 tgttcagcct gatattttaa ttcttgaagg gttaaatgtc ttacagagcg ggatggatta
     1981 tccacacgat ccacatcatg tatttgtttc tgattttgtc gatttttcga tatatgttga
     2041 tgcaccggaa gacttacttc agacgtggta tatcaaccgt tttctgaaat tccgcgaagg
     2101 ggcttttaca gacccggatt cctattttca taactacgcg aaattaacta aagaagaagc
     2161 tattaagact gccatgacat tgtggaaaga gatcaactgg ctgaacttaa agcaaaatat
     2221 tctacctact cgcgagcgtg ccagtttgat cctgacgaaa agtgctaatc atgcagtaga
     2281 agaggtcaga ctacgcaaat aattttcagg ggagctaatg ctcccctttc ttatttttct
     2341 gcactacgca gggatatttc accgcccatc cagggtttta ttattccatc ctgctcaagt
     2401 aataaagcac cctgtttgtc tattccgcgt gaaatgccaa atatttcttt atcaccaatg
     2461 ataagtttca ctgggcgatt aataaaatta tccaactttt cccagcgcga aagataaggt
     2521 gtcaatcctt cttgctcgaa gagttccagc gccgcgcgta attcacgtat cagcatggcc
     2581 gcaagagtat tacgatcgag attgatcccg gcttcctgca gcgtgatcca cccttgatta
     2641 acgacactct cttcaacacg acgcattgcc atgttgatcc ctgcaccaat gactatttgc
     2701 gccgcatcgc cggttttgcc agtcagctcc acaagaatgc ctgccagctt acgatcctgc
     2761 agatagaggt cattaggcca tttaacacga actttatctg cacccagctt gcgtaatact
     2821 tccgccatca cgataccgat aaccagactt aaaccaatcg ccgccgccgg accctgttcc
     2881 agacgccaga acatcgacaa atataagttc gcgccaaaag gcgaaaacca tttccgacca
     2941 cgacgaccac ggccagcatg ctggtattct gcaacacagg catcgccaga tttaagctct
     3001 ccgatgcggt ccaggagata ctgattcgtg gagtcaataa ctggcagcac ggctacactg
     3061 ccgtcatcca gctgactcaa tatcttttcg gcattaagta actggattgg ctcgggcaag
     3121 ctgtatcctt tacccggaac agtaaataca tcaacgcccc agtcacgcag tgtctgaatg
     3181 tgtttattaa tagccgcccg gctcattccc agcgtttcac ccaactgctc gccagagtga
     3241 aattcaccgt tcgctaacag ggcgatcaat ttcagtggca cggtgttatc cttcatgaaa
     3301 ttgtctccac tgcgctcact tcacctgatg caccaataaa gcggacttca ggctcaagcc
     3361 agacattaaa tttttcaccc actttttgtc ttacatgatg cgccagctgt acaacatctt
     3421 cgctttttgc atcgtgctca tttatgagaa ccagcgcctg ttgacggtgt accgcagccc
     3481 caccaatttg cgtcccttta agctggcact gatcaatcag ccaacccgcg gccagtttta
     3541 ctgaaccatc cacctggggg taatgtgggg ctgttggaaa ttgcgccaac aacgcgttag
     3601 cagtttcggc agaaacaaca ggatttttga agaaactacc cgcattaccg ttcacttttg
     3661 gatcagggag tttggtggtg cgcatatgac acaccgcatc aaatacttgc tgtggcgtta
     3721 ctgttgtagg atccagacga gttaagtcac catacgttag tacaggttgc cactcttttg
     3781 gcagacgcag acctacggct acaatggcga agcgatcctg gtattcatgt ttaaaaatac
     3841 tgtcgcgata gccaaaacgg cactctttgg cagttaagcg tacttgcttg cctgtcgcca
     3901 gttcaacaca atcaacataa gcgcaaactc gctgtaattc tacgccataa gcaccaatat
     3961 tctggatagg tgatgagccg acacaaccag gaattaatgc cagattttcc agaccaggca
     4021 taccttcctg caaagtgtat tttaccaggc gatgccagtt ttctccggct cctacatgta
     4081 aataccacgc atcaggttca tcatgaattt cgataccttt gatccggttg atgatcaccg
     4141 tgccgcgata gtcctccaga aaaagtacat tacttccttc acccagaata agaacgggtt
     4201 gtccttctgc ggttgcatgc tgccaggcat tgagtaattg ttgttcgtct tcggcacata
     4261 caatgtgctg agcattatga tcaatgccaa atgtgttcca gggttttaag gagtggttca
     4321 tagctgcttt cctgatgcaa aaacgaggct agtttaccgt atctgtgggg ggatggcttg
     4381 tagatatgat gacaggaaga gtttatagaa acgcaaaaag gcccagtctt ccgactgggc
     4441 tctt
//
LOCUS       DAZGJT010000199         4407 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZGJT010000199 DAZGJT010000000
VERSION     DAZGJT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.204"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..574
                     /locus_tag="ACHKFK_004711"
     CDS             <1..574
                     /locus_tag="ACHKFK_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO9054861.1"
                     /translation="CKEWFHPAFSNQWWCCPEHGTQLALERRSKEREKAEKAAEKKRR
                     REEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISCGTLTSAQWDAG
                     HYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIGQEAVDEIESNHNR
                     HRWTVEECRAIKAEYQQKLKDLRNSRSEAT"
     gene            618..1106
                     /locus_tag="ACHKFK_004712"
     CDS             618..1106
                     /locus_tag="ACHKFK_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO9054862.1"
                     /translation="MGIRELNLTKEQHDWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSRHAIASYYH
                     RVARPRKMLCRGGGRIQKPSLATCRREVDEILNASLFMIYPVLDSAFKNRKRVEKIKH
                     VA"
     gene            1198..1272
                     /locus_tag="ACHKFK_004713"
     tRNA            1198..1272
                     /locus_tag="ACHKFK_004713"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Asn,seq:gtt)
     gene            1278..1353
                     /locus_tag="ACHKFK_004714"
     tRNA            1278..1353
                     /locus_tag="ACHKFK_004714"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1311..1313,aa:Thr,seq:tgt)
     gene            1357..1431
                     /locus_tag="ACHKFK_004715"
     tRNA            1357..1431
                     /locus_tag="ACHKFK_004715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1390..1392,aa:Gly,seq:tcc)
     gene            complement(1496..1650)
                     /locus_tag="ACHKFK_004716"
                     /pseudo
     CDS             complement(1496..1650)
                     /locus_tag="ACHKFK_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1721..2056)
                     /locus_tag="ACHKFK_004717"
     CDS             complement(1721..2056)
                     /locus_tag="ACHKFK_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO9054863.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            2317..4278
                     /locus_tag="ACHKFK_004718"
     CDS             2317..4278
                     /locus_tag="ACHKFK_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9054864.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVVVKPSDGEGTVISTTSEPEYYFVVALAGQSNGMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGASCNYNDIIPADHCLHDVQDLSKFSHPKASAAQYGCVG
                     QGLHIAKKLLPFIPANAGILLVPCCRGGSAFLAGDEGTFSESTGASETSARWGVDKPL
                     YKDLLTRTQAALKANPKNILLAVVWMQGEFDLKQGAYATQPGLFDSMVEKYRSDLSEF
                     GGQCLGGSPSSVPWICGDTTYYWKQTYSSQYDAVYGAYKTKSAKKIFFVPFMTDENGR
                     NVGTNEPSEDPDVADIGYYGAGGRTDAKTWTTADRKTHFGSWARRGIISDRLATAILV
                     HAGRTAEFITGKQPDTVKPTGPSGEGTEREPEAPVSNRTLMSLLASGEDLASQGWRYY
                     HKPASGDNVNKNIAEAVVSDAGATGGKALQLNKPENHIWFLEHDAAGQGVELLKKGGR
                     VSVRFKLPGSLVPNRFAMGIYWQLSSLPEGVTLAEEGNDMLMSFFLQTDATNLNAMYH
                     KKPNAKLDTFGVFDNGWHTLAFEFAGNNSIQVTPVLDEKRGTPFTLVKSPASGAADKL
                     QLTGILKAATYTLLIDSVKVEVNNADAAA"
BASE COUNT         1161 a          969 c         1195 g         1082 t
ORIGIN      
        1 atgcaaggaa tggtttcacc cggcattctc aaatcagtgg tggtgctgcc cggaacacgg
       61 aactcaatta gcactcgaac gacgaagcaa agaacgagaa aaagcggaaa aagcagcaga
      121 gaagaaacga cgacgagagg agcagaaaca gaaagataaa cttaagattc gaaaactcgc
      181 cttaaagccc cgcagttact ggattaaaca agcccaacaa gccgtaaacg ccttcatcag
      241 agaaagagac cgcgacttac catgtatctc gtgcggaacg ctcacgtctg ctcagtggga
      301 tgccgggcat taccggacaa ctgctgcggc acctcaactc cgatttgatg aacgcaatat
      361 tcacaagcaa tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt
      421 cgaactgatt agccgcatcg ggcaggaagc agtagatgaa atcgaatcaa accataaccg
      481 ccatcgctgg actgtcgaag agtgcagggc catcaaggca gagtaccaac agaaactcaa
      541 agatctgaga aacagcagaa gtgaggccac atgacgctct cagtaaaaac cattccagat
      601 aacaagggag aagtcgcatg ggcataagag aactaaacct caccaaagaa cagcacgatt
      661 ggctgaatgg ctggcttgaa ctgtggggcg catgggttta ttcaggtcgt ctggaaaagc
      721 gcatgagcag cgtaatagcg aagttcatgg agagcgtaga gccgggaaga gttatgacaa
      781 gaccaatgtg caatgatgat gatggaatgt tgatttctca ggtcgtcgat tccgtcatgt
      841 acattgacaa gaaagccttc ggcatcctcc tcagctacta cgctcatggt tcttccaggc
      901 acgctattgc atcttactat catcgcgtcg caagacctcg caagatgtta tgccggggcg
      961 gcgggcgcat tcaaaaacca tcgctcgcaa cctgtcgacg ggaagttgac gaaatcctta
     1021 atgcctcgtt gtttatgatt tacccggttc tggatagtgc gtttaaaaac cggaaacgtg
     1081 tagagaaaat taaacatgta gcatagaacg tgttgacatc attgagcaaa tgagcaacac
     1141 tattggcata agctgccgtt agtgactctt aagttgcaac ggtggctttt tttatttggg
     1201 tcagtcgtat aaaggtcatt acggaaggct gttaaccttc ttatcgtggt tcgagtccac
     1261 gctgtcccgc caaacatgct ggtttagctc caatggtaga gcaactgact tgtaatcatc
     1321 aggtcgccag ttcgattccg gtagccggca ccatatgcgg gtaccgtata atggctatta
     1381 cctcagcctt ccagtctgat gatgcgggtt cgattcctgc tacccgctcc agatttatta
     1441 tcaggctcgc ttcggcgggc ctttttttta aatgtctcac aattcagacg gttgacagtt
     1501 gtctgttttg cggggaattt gttaaaagaa actggcatgg tgaatccccc tgtgcggagg
     1561 ggcaatcagc gagtaggtat atgggataat cgcggattca ggtgctggta ctgaattcac
     1621 cgggaggcac ccggcaccat gcaatggcac atagcgccac tctccagccc ctctccggag
     1681 gggctgttta tattgatttt gtcagatgtg agtaaactcc ttatggactt tgttgtttta
     1741 gtccataagg acatatttgc agagtgcaac ggttattaaa gcattcattc aatacgttat
     1801 ctgtatttgt agggcattcc tggctgtttt tgattaaatt ccagaatgtt ttattgaatg
     1861 gtactacgtt gtaaatggtt acaggtagca ctttgttatt gagcatgata cctgtgtgag
     1921 tcagtgtaaa tatactttca ggaggtaaga aagcatccga ttgataccag attattaatt
     1981 ttattttact ccatatgact gaaaaagata ttccgcatga tggctggata actgtatcaa
     2041 tcacaatcca cttcatttag tttccttgtt tatgtcttgc tggtgatgtt ctgaaaagta
     2101 taaatgatat ttttgaatgt aaaccataga gcagaattat ttttctgatg ttgtttattg
     2161 tttatttaaa tgcagggtgg tttatatctc gtcttgtagt ttacccatgc atatctgctt
     2221 gatgatgagg tttttattta aggtatggtt ttgtgttttt tctgtattac atgtcaggta
     2281 ttttaaagaa ttatttttca gatggtggaa agaaccatgg catttaaaca ctatgatgtt
     2341 gtcagggcgg catcgccgtc agaccttgcg aaacgactga cacaaaaact gaaggagggg
     2401 tggcagccat ttggcagtcc ggtggccatc acgccctata ccctgatgca ggccattgcg
     2461 gcggaaggtg atgtcaccac acctgtggtg gtgaagccgt cggatggaga aggcacagtt
     2521 atcagcacca ccagcgaacc ggagtattac tttgttgttg ccctggccgg gcagtcaaac
     2581 ggtatggcgt atggtgaagg gcttccgctg ccggagacat atgaccgtcc ggacccgcgt
     2641 attaaacagc tggcgcgtcg cagcactgtc acgccgggtg gtgcgtcctg taactacaat
     2701 gacattattc ctgcggacca ctgcctgcat gatgttcagg atttgagtaa gttttcacac
     2761 ccgaaagcca gcgcagctca gtatggatgc gtggggcagg gattacatat cgcgaagaaa
     2821 ttgttgccgt ttattccggc gaatgccggt attcttctgg ttccgtgctg ccgtggtggt
     2881 tctgcatttt tggcgggcga tgaaggtacc ttcagcgaat ccaccggcgc aagcgagacc
     2941 tcggcacgtt ggggtgtaga taagccactg tacaaggacc tgcttacccg tactcaggcc
     3001 gcactgaagg ccaaccctaa aaatattctg cttgcagtgg tctggatgca gggcgagttt
     3061 gatttgaaac agggtgcata cgccactcag ccggggctgt ttgattccat ggtggaaaaa
     3121 tatcgttctg acctgtcgga attcggaggc cagtgtctcg ggggctctcc gtcatcggtt
     3181 ccctggattt gtggcgacac gacctactac tggaagcaga cttattcttc gcaatacgat
     3241 gcggtgtatg gtgcatacaa gacgaaatcc gcaaaaaaaa tcttctttgt gccgtttatg
     3301 acggatgaaa acgggcgaaa tgtgggtacc aacgagccgt cagaagatcc ggatgttgcg
     3361 gatattgggt attacggagc cggtggtcga acggacgcca aaacctggac gacggccgac
     3421 cgtaaaacgc attttggatc atgggcacgt cgtgggatta tttccgaccg tctggcaacg
     3481 gcgattcttg tgcatgccgg gagaaccgct gaattcatta ccggaaaaca gcctgatacg
     3541 gtgaagccca ccggaccttc cggtgaaggt acggagagag agccggaagc cccggtcagt
     3601 aaccgaaccc tgatgagtct gctggcgtcc ggcgaagacc tggcatcaca gggctggcgc
     3661 tattatcaca aaccggcgag cggagacaat gttaacaaaa acattgctga agcggtggtc
     3721 agtgatgcgg gggctacggg aggtaaggcc ctgcaactga ataaaccgga aaaccacatc
     3781 tggtttctgg agcatgatgc agccggacag ggagtggagt tactgaagaa ggggggacgt
     3841 gtgagcgtac ggtttaagtt gccgggttca ctggtgccga atcggtttgc catgggcatt
     3901 tactggcagt tgtcgtccct gccggaggga gtgacgctgg cagaggaagg caacgacatg
     3961 ctgatgtcct tcttcctgca gacggatgcg acgaacctga acgcgatgta ccacaagaag
     4021 ccgaatgcga agctggatac gttcggggtc tttgataacg gatggcacac actggctttt
     4081 gagtttgccg gaaacaacag cattcaggtg acgccggtac tggatgagaa acgggggacg
     4141 ccgttcacac tggtgaaatc tccggcatca ggggcggcgg acaaactgca actgacaggc
     4201 atattaaagg cggcgacata tacgctgctg attgacagtg tgaaggtgga agtgaacaac
     4261 gcggatgccg cggcatgata aaaaaagccg ccagcggcag gaatggaagc tggcggaggt
     4321 aatcccaatg gagaatgtaa agaaaagatg ctttcgtata tcggtctttt aaatgaaaac
     4381 agttctcatt gtcaaccata acggtaa
//
LOCUS       DAZGJT010000200         4404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZGJT010000200 DAZGJT010000000
VERSION     DAZGJT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.205"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..369)
                     /locus_tag="ACHKFK_004719"
     CDS             complement(40..369)
                     /locus_tag="ACHKFK_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000042918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9054865.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            complement(781..1752)
                     /locus_tag="ACHKFK_004720"
     CDS             complement(781..1752)
                     /locus_tag="ACHKFK_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HFO9054866.1"
                     /translation="MKRAPVIPKHTIKTQPLEDTPSSAPAAPMVDSLIARVGAMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKSSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQRAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRYQFAPGATVLYKGDKMVLNLDRSRIPTECIERIEAILKELEKTAL"
     gene            complement(1752..2918)
                     /gene="sopA"
                     /locus_tag="ACHKFK_004721"
     CDS             complement(1752..2918)
                     /gene="sopA"
                     /locus_tag="ACHKFK_004721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HFO9054867.1"
                     /translation="MRLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQELALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            3192..4223
                     /locus_tag="ACHKFK_004722"
     CDS             3192..4223
                     /locus_tag="ACHKFK_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFO9054868.1"
                     /translation="MPIIAPIPRTERRLMQKTIHKTKDKNHTRRLTAILMLHRGDTVC
                     HVARTLCCARSSIGRWINWFTLSGAEGMKSLPSGRGRRWPFEHICALLRELIKHSPGD
                     FGYQRSRWSTELLAIKIHEVTGCPLHASTIRRWLPAAGLVWRRATPTLRIRDPHKEEK
                     MVAIHEALAKCSAENPVFYEDEVDIHLNPKTGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSYVGGNSKGSSLFSSLLKHMKATYRRAKTITLIVDNYIIHKSRETLRWLK
                     ANPKFRVIYQPVYSPWVNHVERLWQALHETITRNHQCRSMWQLSKKVRHFMDTVSPFP
                     GGKHGLAKV"
BASE COUNT         1250 a         1057 c          990 g         1107 t
ORIGIN      
        1 ttggttacag actcataccc cgtattcgca acctcattat caggactgag catatttgag
       61 tgccctgaag acatcggtaa gttgtgacgg ctgacagcac atccgtactc cgccgggaag
      121 gaagagcgtg actggctcat tcgtgacggg ctgtggtaca tcagcatgca gtggtggcgc
      181 aatatgaacg ggaagaacgg tcggttcatt tgcccggcgt tcagcttttg cgacatcctg
      241 cggccattcg cgaagtgatg tgaagggaat atcgttgagt tcacagtatt gctgacgtgt
      301 aagaccactg gcgcgccatg cagcaacgtg ctgccttttc tgttcaatgg tccatcgggg
      361 ttttgacatc gattatctcc ggtaagttgg tgaatggatg tcggtaatac taactgaccg
      421 aagatccctg tgaagatgac ttccccgtaa cgacacatgg aatcgtagtc tcactctgat
      481 aatcagatcg acaatacaag tgagaccgtg gtcccagact gattatcaga ccaacaatac
      541 gagagggacc gcgatcccag actgattatc agactaacaa tatgagtggg accgtggtcc
      601 cagagcgata atcagaccaa caatatgagt gggaccgtgg tcccagactg attatcagac
      661 cgacaatatg agtgggaccg tggtcccaga tataatattc aggccagtta gttatgtttt
      721 ctagcctgtg ctaaaggaaa ttaagtaaag acagataaat gtagactaaa acgtgccgca
      781 tcaaagtgcc gttttttcaa gttccttaag aatggcttca attctctcta tacactcagt
      841 tggaatacga gatctatcta ggtttaacac cattttatcg cccttgtaca acactgtcgc
      901 tccaggagca aactgatatc gtgagcttaa gctagttctt gaggcagatg acgttttaag
      961 cacagacgtt aaaagagtaa tgacttcttc agcttcaaat atcactcctg cttttttctg
     1021 ctcgtgaagg ttagatgcct gctgcttaag taattcttct ttatctgtaa aagctctttg
     1081 aagtgcatca cctgaccggg cagatagttc accgggatga gaaaaaagag caacaactga
     1141 tttaggcagt ttagctgtgt tgatacagcg agtaataatc ttacgtgaaa tattttctgc
     1201 atcagccagc gcagaaatat ttccagcaaa ttcattctgc aatcggcttg cataacgctg
     1261 accacgttca taagcacttg ttggcctata atcgttaccc aatctggaca atgcagccat
     1321 ctgctcatca tccagctcac caaccagaac tcgataatca ctttctgtaa gtgcagcagc
     1381 tttacgacgg cggctcccat cggcaatttc tatgacccca gacactcttc gaccgaatgc
     1441 tggtgtctgc tgacctgtca gtagaaaaga agggataaga tcatccaggg cgtcttcggt
     1501 aagcagttcc tggtcacgtt cattacctga ccatacccga gagcttttct caacactatc
     1561 tccccggagc acctcaaggg taaatttcac gtccctacca catacaggca aagtaatagc
     1621 attgccacga gccatagctc ctacgcgcgc aattaacgaa tccaccatcg gtgcagctgg
     1681 tgccgatgac ggagtatctt caagcggttg agtcttaatc gtatgttttg gaatgacagg
     1741 cgcacgcttc attatctaat ctcccagcgt ggtttaatca aacgatcgaa aatttcattg
     1801 cagacaggtt cccaaataga aagggcattt ctccaagcac ctgttgaaga gcgttgatca
     1861 atagcctgtt caaaaacagt tctcatccgg atctgacctt taccaacttc atccgtttca
     1921 cgcacaacat tttttaggac catgcttccc caggcgtccc gaatttgttc ctccatccac
     1981 ggggactgag aaccattact attactgtat ttggtaagca aaatacgtac atcaggctcg
     2041 aatcctttaa gatctacgtt tttgagcaga tcacgaagca tatcgaaaaa ctgcagagcg
     2101 gaagtgtagt cgaacaactc agcaggcgtg gggacaatca acacatcagc agcacataca
     2161 acattaatcg tgccgatacc taggttaggc gcgctgtcaa tgacaatgac atcatagtca
     2221 tgagcgacgg tttcaatggc cagtcggagc atcaggtgtg gatcggtggg caatttacct
     2281 tcatcaaatt tgcccattag ctcagtttca atgcggtgca gagccaaaca ggaaggaata
     2341 atgtcaagcc caggccagca agtaggcttt attgcataag tgacatcgtc cttttcccca
     2401 agatagaagg gaaggagagt atcctctgca tgaatatgaa gatctggcac ccagccgtga
     2461 tacatcgatg ctgttccctg ggggtcgtta ccttccacga gcagaacacg taatcccttc
     2521 agagccagct cctgagcaag atgaacagaa acagaggttt tgtaaacgcc ccctttatga
     2581 gcagcaactc caatcaccgg cggaaatacg tcttcagcac gtcgtagtct cgtaccaaac
     2641 acgtcacgca tatgattaat ttgctcaatt gtataaccaa cacgttgctc aacccgtcct
     2701 cgtatttcca tatccgggtg cggtagccgg cctgctttct cggcatccct gatagcctga
     2761 gaagatactc caactaaatc cgctgcttca cctattctcc agcgtcgggt gattttcctc
     2821 gcttccggac tgtcatcatt aaactgtgca atggcgatag ccttcgtcat ttcatgacca
     2881 gcgtttatac actggttaag tgtttccatg agtctcattc tgaacatcct ttaatcatta
     2941 ctttgcatat tttcatttaa tattgcattt tattgcaaag caacaacaaa attgcaaagt
     3001 agtcaaaaaa acgcaaagtt gtttaaaatg agagcaacac atcaaaaaag gaaacaggaa
     3061 gggcacatac ctcagtcaat aattatcact aaataactgc tcggaatagt agatcactca
     3121 gggggaactc agtccggttt atgcgatctg atcaatagct gaatatccca aatcaccagc
     3181 cggactgagc aatgccgatc atagcaccaa tacccagaac cgaacgacgc ctgatgcaga
     3241 aaactatcca taaaacaaag gacaaaaacc acacccgccg gctcaccgcc atactgatgc
     3301 tgcatcgtgg tgacaccgtc tgtcatgttg ccagaacgct ctgctgtgcc cgctcatcga
     3361 ttggtcgctg gattaactgg ttcacgctgt ccggtgctga aggcatgaag tcattaccgt
     3421 caggacgtgg gagacgctgg ccgttcgaac atatttgcgc attgctgcgt gagcttatta
     3481 agcattctcc cggtgatttc ggttatcagc gttcccgctg gagtactgaa ctgctggcga
     3541 taaaaatcca tgaggtcacc ggttgtccac tacatgcttc aaccatccgg cggtggttac
     3601 ctgcagccgg actggtatgg cgcagagcca cgccgacact gcgtatccgt gacccacaca
     3661 aagaagaaaa aatggtcgct atccatgaag cactggcgaa atgcagtgcg gaaaaccccg
     3721 tgttttacga agatgaagtg gatattcacc tcaatcccaa aactggcgca gactggcagc
     3781 tacgtgggca gcaaaagcgc gtggtcacgc cgggccagaa tgaaaagtac tatctcgccg
     3841 gtgcgctgca cagcggtacg ggaaaagtca gctacgttgg tggaaacagt aaaggttcgt
     3901 ccttatttag cagtctgtta aaacatatga aggcgacata ccggcgggca aaaacgatta
     3961 cgctcattgt cgataactac atcatccaca aaagccgtga aacgttgcgc tggctgaaag
     4021 caaacccgaa gttcagggtc atttatcagc cggtttactc accgtgggtc aatcatgtgg
     4081 aacgattatg gcaggcgctt catgagacaa ttacccgaaa tcaccagtgc cgttcaatgt
     4141 ggcaactgtc gaaaaaggtt cgtcacttta tggataccgt tagcccattc cccggaggga
     4201 aacatgggct ggctaaagtg tagcgatatt aggctcagtt atttagtaga tgcgagtgtt
     4261 aaaaagaaat acttggaata agtacagcac attaggctaa aataacaaat atagaatgag
     4321 attatttttt tcgagtgctt aaaatgatat tttcatagcg aatgacaaca aatatatata
     4381 gtagcataaa aaacaattaa tatt
//
LOCUS       DAZGJT010000201         4307 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZGJT010000201 DAZGJT010000000
VERSION     DAZGJT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.206"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..787)
                     /locus_tag="ACHKFK_004723"
     CDS             complement(<1..787)
                     /locus_tag="ACHKFK_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HFO9054869.1"
                     /translation="MKINKILSSATLLYGMSMAMSVGSCATPVQTNLPGYTPGADIIS
                     VSPTRNQVDLIGDVVYSQIKGTRSVRQLHMSVLVPRTNDLKPAIIYYPGGGFMSSEHD
                     KFIEMRMALAEAGFVVAAVEYRTIPDTFPAPVEDGKAAIRYLREHASNYGIDPQRIGV
                     LGDSAGGWLAQMMGTTNGDKTFDKGDFLQQSSDVQAVATLYGISDLLNIGEGFPESVQ
                     EVHRSPAVTEALMINGPAFRNFAGAPITASKEKALNASPIGHMK"
     gene            1137..1238
                     /locus_tag="ACHKFK_004724"
     CDS             1137..1238
                     /locus_tag="ACHKFK_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054870.1"
                     /translation="MRTYNPTYLLPSQMQKCTCDILHPAFDLCGGEA"
     gene            1235..1690
                     /gene="ybcN"
                     /locus_tag="ACHKFK_004725"
     CDS             1235..1690
                     /gene="ybcN"
                     /locus_tag="ACHKFK_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA base-flipping protein YbcN"
                     /protein_id="HFO9054871.1"
                     /translation="MNFPQDGIKLHRGNFTAIGRQIQPYLEDGKCFRMVLKPWRERRS
                     LSQNALSHMWYSEISEYLISRGKTFATPAWVKDALKHTYLGYETKELVDVVTGEITTI
                     QSLRHTSDLDTGEMYVFLCKVEAWAMNIGCHLTIPQSCEFQLLRDKQEA"
     gene            1690..1860
                     /locus_tag="ACHKFK_004726"
     CDS             1690..1860
                     /locus_tag="ACHKFK_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO9054872.1"
                     /translation="MATPLIRVMNGHIYKVPNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            1853..2143
                     /locus_tag="ACHKFK_004727"
     CDS             1853..2143
                     /locus_tag="ACHKFK_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFO9054873.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTHFVDAGYAKECALEGMARTQVIWLKEGVIEA"
     gene            2140..2502
                     /gene="rusA"
                     /locus_tag="ACHKFK_004728"
     CDS             2140..2502
                     /gene="rusA"
                     /locus_tag="ACHKFK_004728"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HFO9054874.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHVSAEGQAYCDNVARIIKN
                     AMLDIGLAMPVKISIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGKLELTITELGDE"
     gene            2499..2639
                     /locus_tag="ACHKFK_004729"
     CDS             2499..2639
                     /locus_tag="ACHKFK_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO9054875.1"
                     /translation="MMFESYMAERLRRRWVRLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            2725..3108
                     /gene="quuD"
                     /locus_tag="ACHKFK_004730"
     CDS             2725..3108
                     /gene="quuD"
                     /locus_tag="ACHKFK_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO9054876.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSVAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVCGMTFMSLASKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLDMDIVANNSN"
     gene            complement(3297..>4307)
                     /gene="ompC"
                     /locus_tag="ACHKFK_004731"
     CDS             complement(3297..>4307)
                     /gene="ompC"
                     /locus_tag="ACHKFK_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO9054877.1"
                     /translation="EIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYARLGFKGETQ
                     INDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRNYGVAYDIG
                     AWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQGKNDRTDV
                     TEANGDGFGFSTTYEYEGFGVGATYAKSDRTDGQVAYGKSKFNASGKNAEVWAAGLKY
                     DANNIYLATTYSETQNMTVFGNNHIANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKDL
                     GVHGDRDLVKYVDVGATYYFNKNMSTFVDYKINLIDDSKFTKTAGIDTDDIVAVGLVY
                     QF"
BASE COUNT         1168 a          951 c         1015 g         1173 t
ORIGIN      
        1 ctttcatatg tccgattgga ctggcgttta gcgctttttc ttttgacgct gtgatggggg
       61 ctcccgcaaa atttctgaat gcagggccat tgatcattaa ggcttcggtt acggcaggag
      121 agcgatgaac ctcctgcact gattcaggga acccctcgcc aatattcaac aagtcagaaa
      181 tcccataaag tgtggcaact gcctgaacat ctgaggattg ctgaagaaag tcacctttat
      241 caaaggtttt gtcaccattt gtagttccca tcatctgagc aagccatcca ccggcagagt
      301 cacccagaac tccgattctt tgaggatcaa tcccataatt gctggcatgc tctctcaggt
      361 aacgtattgc agcttttcca tcctcaactg gtgctggaaa tgtatcagga attgttctgt
      421 attctacagc ggccacaaca aaaccagctt ctgccagagc cattctcatt tcaataaatt
      481 tgtcatgttc agaagacatg aatccgccgc cgggataata aataatggct ggttttaaat
      541 catttgttcg cgggacaaga actgacatgt gaagctgtct gacagaacga gttcctttta
      601 tctgggaata aacaacatca ccaatgagat cgacctggtt tctggtcggt gaaacactaa
      661 tgatatctgc acccggggtg taaccaggaa gattagtctg gacaggtgtt gcacaactcc
      721 cgaccgacat ggccattgac ataccataca atagtgttgc agatgataat atcttgttaa
      781 ttttcatgtt ttacatgcct tgacttatcg gatgaaattc tcttactgaa tttaacggca
      841 gtttaatagc cgtacaactc gtggtagcca gggaaatatc ccagtcgtgg ttaaggttgt
      901 acttgacggc tattatttca acaatgctta aagtgagagc ttaaggttat gcgtatgttt
      961 tacagtccga gtagattctg accaatagct cctttgtata attctgtcag aaataaatct
     1021 tggcttgcat gaagtttgag accttatctt gtttgattat gaataatcaa ttcgccataa
     1081 ttgtatcacc ggagcctgaa caactccggt gacttctgcg ctaaacgggg acgtttatgc
     1141 gcacatacaa tccaacctat cttctccctt cacagatgca gaaatgcacc tgcgatatct
     1201 tgcatccagc gtttgatctc tgcggaggtg aagcgtgaac ttcccacaag atggcatcaa
     1261 actgcatcgc ggtaacttca ccgctatcgg tcggcagatc cagccttatc tggaggacgg
     1321 caaatgcttt cgcatggtgc ttaaaccatg gcgcgagaga cgcagtcttt cccagaatgc
     1381 actcagccac atgtggtaca gcgaaatcag tgaatacctc atcagcaggg gtaaaacgtt
     1441 cgccactcca gcttgggtaa aagatgctct caaacacact tatctcggtt atgaaaccaa
     1501 agaactggtt gatgtcgtaa ccggtgaaat caccaccatt cagtcattac gtcatacctc
     1561 cgatctcgat accggagaga tgtatgtctt cctgtgtaag gttgaagcct gggcgatgaa
     1621 tattggctgc cacctgacta ttccgcagag ctgcgagttc cagctgctgc gcgacaagca
     1681 ggaggcgtaa tggctacacc gcttattcgt gtcatgaacg gacacatcta caaagtacca
     1741 aatcgtcgta agcgtaaacc tgagctgaag ccatccgaaa taccaacact gctcgggtat
     1801 accgccagcc tggttgataa aaaatggttg cgactggcag caaggaggaa tcatggctga
     1861 tttaagaaaa gcagcgcgtg gtcgggaatg ccaggtaaga atccctggcg tatgtaatgg
     1921 caatcctgaa acgtcagtac tggcacatat ccggctggct ggattgtgcg gtaccggtat
     1981 caaaccgcca gacctgattg ccaccattgc atgttctgcc tgccacgacg aaatcgaccg
     2041 tcgcacgcat tttgttgacg ctggatatgc aaaagaatgc gcgctggaag gtatggcgag
     2101 aacacaggtt atctggttga aagagggggt tattgaggcg tgaataccta cagcatcaca
     2161 ttaccctggc ctccgagcaa taatcgctat taccgccata atcgtgggcg cacgcacgtc
     2221 agcgcagagg ggcaggcata ctgcgataac gtcgcccgaa tcattaaaaa cgcaatgctg
     2281 gatatcggct tggctatgcc agtgaaaatc agcattgagt gccacatgcc ggatcgccgt
     2341 cgccgtgacc ttgataatct gcaaaaggcc gcttttgacg cactcaccaa agcaggtttc
     2401 tggctggatg atgctcaggt cgttgattac cgcgttgtga agatgcccgt taccaaaggt
     2461 gggaagctgg agctgactat cactgaactg ggagatgaat gatgtttgag tcttatatgg
     2521 cagagcgtct tcgccgccgc tgggtgcgcc tgcgcttata tcgttttcct ggttctgttt
     2581 tgaccgatta ccgaatactg aagaattacg ccaaaaccct gacaggagca ggagtatgaa
     2641 gtcagagata acaatcaact aatactgttt tgttgatttt tgcttgtaat tggcgttctg
     2701 gtctgatttt tgtggagtaa gttgatgcgt gatattcaga tggttcttga gcgttgggga
     2761 gcgtgggcgg ctaataatca tgaagatgtg acctggtcgt ccgttgccgc cggttttaag
     2821 ggattaattc cttcaaaagt aaaatctcgc ccgcaatgtt gtgacgatga cgcgatgatc
     2881 atttgcgggt gcatggcccg tctgaaaaag aacaacagcg atttacacga tttattagta
     2941 gattattatg tatgtggtat gacattcatg tcactggcaa gtaagcattg ctgctcggat
     3001 ggttatatcg ggaaaaggtt acagaaggct gagggcataa ttgaagggat gttaatggca
     3061 ttagatatcc ggttagatat ggatatcgtt gctaataatt ctaattgata tgcaattgtt
     3121 tactaaaagt tattaaaaat ggggcgtgga aacgccccca aaataaaggg taatatataa
     3181 cagaaggttt atatagtaag aagcaaggta gtgcttctaa aggaagtggc ttgagggctc
     3241 cacttatatg ttgcggaggc aaagcctccc gcaacatatc tttttcgtaa gtcagattag
     3301 aactgataaa ccagacctac agcgacgatg tcgtcggtat caataccagc tgttttggta
     3361 aacttactat cgtcaattaa gttgattttg taatcaacaa aagtggacat gtttttatta
     3421 aagtagtaag tagcaccgac atcgacatac ttgactaagt ctcggtcacc atgaacacca
     3481 aggtctttac cttttgactg aaggtaagca acagatggtc gcagaccgaa gtcaaactga
     3541 tattgtgcta ctgcttcaaa gttttgtgct ttgtttgcaa tatggttatt accaaaaacg
     3601 gtcatattct gagtttcaga atatgtggta gccagataga tattgttcgc atcatatttc
     3661 aggcctgcag cccatacttc cgcatttttg ccggaggcat tgaatttgct cttaccatag
     3721 gcgacctgac cgtcagtgcg atctgattta gcataggttg cacccacgcc gaatccttca
     3781 tactcataag tagtggagaa accgaaacca tcaccattgg cttcagttac gtcagtgcgg
     3841 tcatttttac cctgatactg agcagcaaag ttcaggccat cgaccagacc aaagaagtcg
     3901 ttgttacgat aagttgcaac accagtagtg cgaccagtca tgaacacatc tgtttgggtc
     3961 caggtatcac caccgaattc tggcaggacg tcagtccacg caccgatgtc gtatgctaca
     4021 ccgtagttac ggccgtaatc gattgagccg taatcaccaa atttcaggcc tgcaaatgca
     4081 agacgggttt tgtctttgga agaaccttga gattcagcac ggttgccttt gaattcatat
     4141 tcccactgac cgaaaccagt cagttgatcg ttgatttggg tttcaccttt gaagccaaga
     4201 cgggcataag tagtatcacc atcatctgca tcattagagg agaagtagtg cttggcatta
     4261 actttcccgt atagatccag cttgttactg tctttattat aaatttc
//
LOCUS       DAZGJT010000202         4260 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZGJT010000202 DAZGJT010000000
VERSION     DAZGJT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.207"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..240)
                     /gene="ariR"
                     /locus_tag="ACHKFK_004732"
                     /gene_synonym="ymgB"
     CDS             complement(<1..240)
                     /gene="ariR"
                     /locus_tag="ACHKFK_004732"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO9054878.1"
                     /translation="MLEDTTIHNAISDKALSSYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIV"
     gene            complement(269..541)
                     /gene="ymgA"
                     /locus_tag="ACHKFK_004733"
     CDS             complement(269..541)
                     /gene="ymgA"
                     /locus_tag="ACHKFK_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFO9054879.1"
                     /translation="MKTSDNESIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     ENTFRRDLIDSIIRDFSNSDTGGPNRRTAIADNKSMFTGKKINRIH"
     gene            complement(584..820)
                     /gene="ycgZ"
                     /locus_tag="ACHKFK_004734"
     CDS             complement(584..820)
                     /gene="ycgZ"
                     /locus_tag="ACHKFK_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO9054880.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQENLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
     gene            1134..2345
                     /gene="bluF"
                     /locus_tag="ACHKFK_004735"
     CDS             1134..2345
                     /gene="bluF"
                     /locus_tag="ACHKFK_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO9054881.1"
                     /translation="MLTTLIYRSHIRGDEPVKKIEEMVSIANRRNMRSDVTGILLFNG
                     SHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTAD
                     LKSKALAFAMAHALKLGDKMISINLLPMTLVNKPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLN
                     GIPSVAWPEKK"
     gene            2550..3281
                     /gene="bluR"
                     /locus_tag="ACHKFK_004736"
     CDS             2550..3281
                     /gene="bluR"
                     /locus_tag="ACHKFK_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO9054882.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTQDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            3502..3906
                     /gene="ycgX"
                     /locus_tag="ACHKFK_004737"
     CDS             3502..3906
                     /gene="ycgX"
                     /locus_tag="ACHKFK_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO9054883.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIRGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            3959..4084
                     /locus_tag="ACHKFK_004738"
     CDS             3959..4084
                     /locus_tag="ACHKFK_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054884.1"
                     /translation="MEIKVQRLSLWMINTVFLLLPINNHQTNTIKLIFEMCSLDF"
     gene            complement(4168..>4260)
                     /locus_tag="ACHKFK_004739"
     CDS             complement(4168..>4260)
                     /locus_tag="ACHKFK_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054885.1"
                     /translation="KTVTYDFERLMEGAKLLKCSEFGDAIIKNM"
BASE COUNT         1259 a          880 c          842 g         1279 t
ORIGIN      
        1 cacgatttcc agtgtttttc taatcacatc agctttgaga atatcacttg ttgtttccag
       61 ggagtgtatc agacttaaga taatattttt attattaaca ttatcgcctg aaagcattaa
      121 attggtcaca gcctgcccta acacagcgga ctcttcttct aacaaattac ctgaactacg
      181 aaagtaactt gataacgctt tatctgatat tgcattatga atagtagtat cttcaagcat
      241 ggtaacctct catcttactt atgaaatttt aatgtattct gtttattttc ttaccagtga
      301 acatactttt attgtcagct atcgctgttc ttcggtttgg ccctcctgta tcactgttgc
      361 taaaatcacg aattatgctg tcaattaaat ctcgtcggaa agtattttcc tcagccgatt
      421 tcattacgag taattgctta ataagagtct gcaacgaaaa atttatcttc atgcgcagta
      481 tctggagcac cgcctggcca gtaatttcat attttatact ttcattatca gatgtcttca
      541 taacgacttc atagctaacc tgttaaataa tcaggctgtt gcattattca aaaagcaacc
      601 caattagtgc attataatgt ttctgttcct cttcccctgt cgcttgttcc agccggcaaa
      661 gaagtttggc acaaagcgac tttcgactca gattacgtcc atctttcaaa atctcagtca
      721 caatttcccc gagattttcc tgctgagtag gcaagttagc ttttgcaaag taacgagtga
      781 ttgctcctgc agaatctgaa gtcactgaat tttgatgcat gctacgcctc tgttaaaaat
      841 gttaatcaat gttaagtgag gtaacaatag caaacttgta cgaaatatgt gtacagaatg
      901 ataaattaat ttttcgcaat tagtgctaat tcatataaaa caatgcatta cgataaaaca
      961 tattgcatca tttaaatgac aacctataca ggttttttcg ccaaaccgtc ttcaaccttt
     1021 tccacttttt atcaatgtaa agaaacggtt atgttttggt attaaaagaa agacacaaga
     1081 agatatgtct gttaccgtct tactctcacc tcgcccatta ccctggattg ttaatgctta
     1141 ccacccttat ttatcgtagc catatacgtg gcgacgaacc tgtcaaaaaa attgaagaaa
     1201 tggtttcgat agcaaatcgc aggaacatgc ggtctgacgt aacagggatc ttactgttta
     1261 atggttctca ttttttccag cttctggaag gtccggaaga acaggttcaa atgatatatc
     1321 gggccatatg ccaggatcca cggcactata atattgttga actgttgtgc gattacgcgc
     1381 ctgctcgccg ttttggcaaa gcaggaatgg aattatttga tttacgcctg cacgagcgag
     1441 atgacgtttt acaggccgta ttcgacaaag gcacatcaaa atttcagcta acttatgatg
     1501 acagagcgct acaatttttt cgtacttttg tccttgcaac tgaacaatca acctatttcg
     1561 agattcctgc tgaagactcc tggcttttta tcgctgacgg atctgataaa gaacttgatt
     1621 cttgtaccct ttcatcaaat ataaacgacc actttgcctt tcatcctatt gtcgaccctt
     1681 tatcgcggcg gataatcgct tttgaagcca ttgtgcaaaa aaatgaagac agcccgtcag
     1741 ctatagcggt tgggcagcgt aaagacgagg aaatctacac agcggatctc aaaagtaagg
     1801 cgcttgcatt tgcgatggca cacgcactta agctcggtga taaaatgatt tcaatcaatc
     1861 tattacctat gaccctggtt aacaaacctg acgcagtctc ttttttactt gatgaaataa
     1921 aggccaatgc tctggtacct gaacaaatca tcgttgaatt tactgaaagt gaagtcatat
     1981 ctcggtttga tgagtttgcc gaagcggtta aatcgctaaa ggcggccggt atcagtgtag
     2041 caattgatca ttttggcgca ggttttgctg gtttgttact cctgtcacgc ttccagcctg
     2101 acagaattaa aatcagtcag gaattgatta ccaatgttca taaaagcgga ccacggcagg
     2161 caattattca ggcgatcatg aaatgctgta catcacttga aattcaagtc tctgctatgg
     2221 gcgtggcaac accagaagag tggatgtggc ttgaatctgc aggaattgag atgtttcagg
     2281 gagatctgtt ttccaaagct aaattgaatg gtatcccttc agttgcgtgg ccggagaaaa
     2341 aataattttc agcacattat ttcgcatgat ttctggaaat catcgtgaaa atgaatacgt
     2401 tatgttttaa tttaatccac attaaaaata atgtttcatc caatttagct gaaatcttat
     2461 ttatactttg tacaatttta gcatatattg tacaaagtat tattgggtcg tgtagaggcg
     2521 acggagattt atgatcgcaa ggaggaattg tggcttatta cagcattggt gatgttgctg
     2581 aacgttgcgg gattaatcct gtcactctcc gggcctggca acgccgctac ggtttgttaa
     2641 aaccacaacg cagtgaaggc ggacaccgac tctttgatga agaagacata caacgcatcg
     2701 aagagattaa gcgttggata agtaatggcg ttcctgtagg caaagttaaa gcattactgg
     2761 aaactaccag ccaggatacg caagatgact ggaaccgcct gcaagaagag atgatgtcaa
     2821 ttcttcgtat ggctaatcca gccaaactac gcgcgcgaat tatttcactg ggtcgagagt
     2881 acccagtaga tcaattgatt aatcatgttt accttcctgt tcgccagcgt ctcgtgcttg
     2941 atcacaacac ctcccgcatt atgagcagta tgtttgacgg cgcattaatt gaatacgcag
     3001 cggcctcact tttcgaaatg cgccgtaagc ccggtaaaga ggccattctg atggcgtgga
     3061 atgttgaaga gagagcacga ttgtggctgg aagcatggcg tttatcattg tcaggatggc
     3121 acatttctgt tcttgctgat cccattgaat cgccgcgccc ggaactgttc ccaacgcaaa
     3181 cattgattgt ctggacaggc atggcaccaa cgagaaggca aaacgaactt ttacaacact
     3241 ggggtgagca aggttataaa gtcatctttc atgcccccta aattcacctt tatcccgccc
     3301 cttagacaga atgttataat ctgaacccac accagcgttt gtagcggagc tggtccgttg
     3361 caaaatgccc accgggagaa ttcaggtaat ccccctttca cactttgtac atatcacagc
     3421 caaaggacag accagattga agtggtaaaa ccgatcgtgc acactacgca gtctcacacc
     3481 taacaagagg ttattatcct gatggatcag gtcgttgttt ttcaaaagat gtttgagcaa
     3541 gtgagaaaag agcaaaactt ctcctggttt tattcagaat taaaacatca ccgtattgca
     3601 cattacattt attatctggc tacggataac atcagaatta ttactcacga tgacacggtt
     3661 ttgttattaa gaggaaccag aaacctgtta aaagttagta cgaccaagaa cccagctaaa
     3721 ataaaagagg ccgcattgct tcatattcgc ggaaaatcta catttcggga atactgttca
     3781 acactggcag gcgctggcgt tttccggtgg gttactgatg ttaatcataa caaacgcagt
     3841 tactacgcca ttgataatac gcttttatat attgaagatg tagaaaataa taaaccatta
     3901 atctagctaa agttgggagc ttaaaaaatg cttcataatt cagtaaggca ttagcataat
     3961 ggaaataaaa gtgcagagac tatccctatg gatgattaat actgtctttt tattgctccc
     4021 cataaataat caccagacca atacgatcaa attgatattt gaaatgtgtt cacttgactt
     4081 ttgatacgtt attttataac ggttaacata tttataaaaa caacgggcgt gttatacgcc
     4141 cgtttcaata tttaacacat gtagagatta catgttcttg atgatcgcgt caccaaactc
     4201 tgaacatttc agcagtttag cgccttccat cagacgttcg aagtcatagg ttacggtctt
//
LOCUS       DAZGJT010000203         4258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZGJT010000203 DAZGJT010000000
VERSION     DAZGJT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.208"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(69..1091)
                     /locus_tag="ACHKFK_004740"
     CDS             complement(69..1091)
                     /locus_tag="ACHKFK_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HFO9054886.1"
                     /translation="MSTMKVLICQEIKNLVCEVRERPVPGDNEALIKIKTVGICGTDI
                     HAWAGNQPFFSYPRVLGHEICGEIIESGKNIHNFNIGQQVAVIPYISCQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APSEQVLVVGAGPIGLGAAAIAKADGAQVVVVDTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            1230..2135
                     /locus_tag="ACHKFK_004741"
     CDS             1230..2135
                     /locus_tag="ACHKFK_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO9054887.1"
                     /translation="MSRSQNLRHSVINQVIEDMARGNIPSPLPSQSGLAEMYNISRTT
                     VRHILQHLSACGVLTLVGKNYVIARNPEQNDGFDCITASLKEQNRLFEQAFFTMINLR
                     QLRAGESFSELQLARAASVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDDDPRWLQAKTMLERHRTLRDSIGSNFRMFSQLDRDFHA
                     MLLSAADNIFFNQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     AITALRNHLDTAKQSMIRSIRQHHR"
     gene            complement(2306..3958)
                     /locus_tag="ACHKFK_004742"
     CDS             complement(2306..3958)
                     /locus_tag="ACHKFK_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO9054888.1"
                     /translation="MEILLTILILTLVVSLTSVLTRLSPVQIPLPLIQIAAGAVLAQP
                     IFGLHVEFNPELFLLLFIPPLLFAESSKIQPKELIKHSREIISLALVLVLITIFGVGY
                     VIHLLLPNVPLIAAFALAAVLSPTDAVALLGIVGKGRISKNIQEVLEGEALMNDASGL
                     VALKFAVAVTMGTMEFSVHGATIAFFVVALGGIAVGIAVTWLYGKGLLLISRYAHDEP
                     SIQIVLMLLLPFIVYLVAEHFGLSGILAAVAAGLTTSRIGVVHHAPIRLRIKAKSSWS
                     MLEYVFNGMVFLILGLQLPGIISESLIRAENDPLVDTGSLLLDVIWIYLALMVVRLGW
                     LWLMRLYSRKRPGKHPMLFAGYKTRHMLLATFAGVRGAITLAGVLSIPLLLPDGTPFP
                     GRYLLVFLATGVILFSLVCGIIILPLLLGKNVGIFSDNRQKEEEAADLFMAKMAILSV
                     QKTEQRLLRARTENLDNELIMETGARVIGYMRHSEEDMSPNDNSLHMQRAKLERTFNL
                     AALNAKRAAIYQMRARQEVSDETLAKIVSELDVLETLIMDSR"
BASE COUNT         1208 a         1090 c          880 g         1080 t
ORIGIN      
        1 aaacagaaaa aaaacagcgc ccaacacttc cccggacgct gcatggcaac ttgaataatc
       61 tcgccttatc agaaagtaat tacgccctta atcaactcac gattgttaat cacatcgcgc
      121 tcgtaggttt ctgccagcgt ggcgaacgga tagcgatggg ttaacatcat gtcggcagtg
      181 attttccctt ccgccatcaa tcgaccgact ttagcgaaat cttccggcgt ggcgttgcgg
      241 ctgcccatca tcgtcgtttc ttttttatgg aattccggat cggagaactg caactcacct
      301 ttaaacaggc cgacaaatac caccgtgccg ccgtgacgaa tcagatttac ggtgttattc
      361 atcgcatgtt gattacctgt cgcgtcaatc actttctgcg ccagcgaacc accaaactgc
      421 gcccgcagct gcgcgtcaaa atcttcggct gacgggtcca gaacaggtaa ttccagacgc
      481 gttgccacat gttcacgacg cgccggactg gtatccacca ccaccacctg cgcgccatcg
      541 gctttagcga ttgccgccgc gcccagaccg attggccccg ccccgaccac cagcacctgc
      601 tcgctgggtg caatggctgc acgacgcacc gcatgagcgc taatagcgaa aggttcaatc
      661 aatgctgccg cctgcgggtc aataccgtct gcgggcaaaa tgttcgccac cggcacgctc
      721 aaatactcac taaaaccgcc atcctgatgc acgccaatga ctgaaatttt ttcacagcaa
      781 ttggtacgac cgcttttaca cgccgggcat tgctgacaag aaatatacgg aattaccgca
      841 acttgctgtc cgatattaaa attgtgaatg ttttttcctg attcaataat ctccccacat
      901 atttcatggc ctaaaacgcg tggataacta aaaaatggct gattgcccgc ccaggcatga
      961 atatctgtac cgcaaatccc gacagtttta attttaatta atgcttcatt atcgcctggc
     1021 accggtcttt cccggacttc acatactaaa ttctttattt cctgacaaat taatactttc
     1081 attgtggaca tgattcaccg ctccttttat tttatgactt tcagatatcg tcgaatttat
     1141 aagagcgcct gaatttaccc ataaaatatg cggatgatcg cgcataaaaa tgttttaaat
     1201 cggcttttaa tgccggactg gagggaagaa tgagtcgttc gcagaattta cgtcatagcg
     1261 tcataaatca ggtgattgaa gatatggcga ggggcaatat tccttctccg ctgccttcac
     1321 aaagcggact ggcagaaatg tataacatca gtcgcactac ggtacgccac atactccagc
     1381 atttaagcgc atgtggcgtg ctcaccctgg tggggaaaaa ttatgttatc gcccgcaacc
     1441 ccgaacagaa cgatggattt gattgcatca cggcctcgct gaaagagcaa aaccgtcttt
     1501 tcgagcaggc atttttcacc atgattaacc tgcgccagtt acgcgccgga gaaagctttt
     1561 ccgaacttca gctggcgcga gcggcaagcg tcagtccggt agtggtacga gaatatcttt
     1621 tgaaattcgg tcgttataat cttattcaaa gcgaaaagcg cggccaatgg agcatgaagc
     1681 agttcgatca atcctacgcc gagcagctgt ttgagctacg cgaaatgctc gaaacacatt
     1741 ccctgcaaca ctttctcaac cttcccgatg atgaccctcg ctggctgcaa gcgaaaacca
     1801 tgctcgaacg ccaccggacg ctgcgcgaca gcattggcag caactttcga atgttttctc
     1861 aattagaccg tgattttcat gccatgctgt tatccgctgc tgacaacatc ttttttaatc
     1921 aatcacttga aattatatcg gtgatctttc attttcacta tcaatgggat gaaagcgatc
     1981 tcaaacagcg caatatcatt gctgttgatg aacatatgac cattctcagc gccctgattt
     2041 gccgtagcga tttagatgca ataacggcgt tgcgaaacca tctggatacc gccaaacaat
     2101 caatgattcg ttccatcagg caacatcacc gataaattaa gtacagatta aaaacattga
     2161 ttcttggttt atatcactat gttgtattaa tgcagtatta atccaggcga ttgttctgcc
     2221 cataaggaaa tacataggga ataaacgatg ctgaaagtat tactctcagc catcatttaa
     2281 ttccctatta ctggcagcga taaaattatc ggctatccat gattaacgtt tccagcacat
     2341 ctaattcact gactattttt gccagtgttt catcactgac ttcttgcctt gcgcgcatct
     2401 ggtaaatagc cgcacgtttg gcatttaacg ccgccagatt aaatgttctt tccagcttcg
     2461 cacgctgcat atgcagggaa ttatcattag gcgacatatc ctcttcacta tgtcgcatat
     2521 aaccgataac tctggccccc gtttccataa tcaactcgtt atcgaggttt tctgttctgg
     2581 cgcgcagcaa acgttgctcg gtcttttgca cactcagtat ggccatttta gccataaaca
     2641 gatcggcagc ttcctcttct ttttgtcggt tatcgctgaa aatccccaca tttttcccta
     2701 gtaataacgg cagaataata atgccgcaaa ccagcgaaaa gaggatcaca cctgtagcga
     2761 gaaataccag caagtaacgc ccgggaaaag gtgtgccatc cggcagcaat aacgggatag
     2821 aaagcacccc cgcaagcgtg atagctccgc gcacccccgc aaaggtcgcc agcagcatat
     2881 gacgcgtttt atatccagca aacagcatcg gatgtttgcc gggtcgttta cggctataca
     2941 gacgcatcag ccaaagccag cccaggcgca caaccattaa cgccaggtag atccaaatga
     3001 catcaaggag cagcgatccg gtgtccacca gtggatcgtt ttctgcacgg atcagtgatt
     3061 ccgaaataat gcccggcagt tgtaagccta aaatcagaaa caccatgccg ttaaagacat
     3121 attcaagcat cgaccagcta cttttagctt taattctcag gcgaataggc gcgtggtgga
     3181 ctacgccaat gcgactggtt gttaaccccg cggcgacagc ggcaagaatg ccagataagc
     3241 caaaatgttc cgcaaccaga taaacaataa acggcaacag aagcatcaat acaatctgaa
     3301 tggaaggttc atcgtgagca tatcggctga tcaacagcag ccctttacca tataaccagg
     3361 taacagcgat ccccacggca ataccgccca acgcaacgac aaaaaacgca atggttgcgc
     3421 catgaacgct gaattccatc gttcccatcg tcactgcaac agcaaatttc aacgccacca
     3481 ggccggaagc atcattcatc aacgcttcgc cttcgagcac ttcctggatg tttttgctga
     3541 tacgcccttt cccgacaatc cccagcaacg ccacggcatc ggtaggcgac aacacggcgg
     3601 ctaatgcgaa agcagcgatg agtggcacat tcggtaagag taaatgaatc acatagccga
     3661 caccaaaaat agtgattagt accaacacaa gtgccaggct gataatttcc cggctgtgct
     3721 tgatcaattc tttaggttgt atttttgaac tttcagcaaa caacaggggt ggtataaaca
     3781 ataagagaaa aagttcagga ttgaattcca catgcagacc aaatataggc tgggccagta
     3841 ctgcaccagc ggcaatctgg attaaaggaa gtggtatctg gacaggagac agacgtgtaa
     3901 gcacgctggt taatgatact accaacgtaa gaattaaaat tgttaaaagt atttccatat
     3961 aataaacagg cttcctcatt cataaataat ggatattatt aatttaactt cataaaaagc
     4021 gctctggtta gcacttaacg ttaatcatta atcactgcgt tcactcacat gatcagaagg
     4081 aacccagtaa aataagtttt caagttgata acaaattcac catgtgaata tttactgcct
     4141 ttccccgttg taacgaatca tccgttactc tctgatttcc ttgccgaaca agttatttag
     4201 tgaatgacgg ctttctttta atatcagcat gaattatacg ttcagcaaat gtattaaa
//
LOCUS       DAZGJT010000204         4243 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZGJT010000204 DAZGJT010000000
VERSION     DAZGJT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4243
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.209"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..1115
                     /gene="rfaD"
                     /locus_tag="ACHKFK_004743"
     CDS             183..1115
                     /gene="rfaD"
                     /locus_tag="ACHKFK_004743"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO9054889.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1125..2171
                     /gene="rfaF"
                     /locus_tag="ACHKFK_004744"
     CDS             1125..2171
                     /gene="rfaF"
                     /locus_tag="ACHKFK_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO9054890.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGVMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGYEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2175..3167
                     /gene="rfaC"
                     /locus_tag="ACHKFK_004745"
     CDS             2175..3167
                     /gene="rfaC"
                     /locus_tag="ACHKFK_004745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO9054891.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAKNYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIIINKLTSEIR"
     gene            3164..>4243
                     /gene="rfaL"
                     /locus_tag="ACHKFK_004746"
     CDS             3164..>4243
                     /gene="rfaL"
                     /locus_tag="ACHKFK_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO9054892.1"
                     /translation="MTSTLFFSFEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLVQSPDAGAGMKEIFKAII
                     ENTLLCTIAIPVILRDEKREDIEKIVFFSFISALGLRCFSELIAYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFAVLSAIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPQWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNVVQNKYRSP"
BASE COUNT         1124 a          968 c         1037 g         1114 t
ORIGIN      
        1 gcactattca cgtgcaaatc caacatccgc catgaaggac tggctaaaac ccaaactagt
       61 ttgttgcaat tagcatcctt gcatctctat gtaaagggct gaagggattc ggatgtgatg
      121 gtatgattac agacattcgt gtctgagatt gtctctgact ccataattca aaggttacag
      181 ttatgatcat cgttaccggc ggcgcgggct ttatcggcag caacatcgtt aaagccctga
      241 atgacaaagg catcaccgat attctggtgg tggataacct gaaagacggc accaagtttg
      301 tgaacctggt ggatctggat atcgcggact atatggataa ggaagacttc ctgatccaga
      361 ttatggctgg cgaagagttc ggcgatgtcg aggcgatttt ccacgaaggc gcgtgctctt
      421 ccaccaccga gtgggacggc aagtatatga tggataacaa ctatcaatac tccaaagagc
      481 tgctgcacta ctgtctggag cgcgaaatcc cgttcctgta tgcctcttcc gcagccactt
      541 acggtggacg cacctccgac tttattgaat cccgcgaata tgaaaaaccg ttgaatgtct
      601 acggttactc aaaattcctg tttgatgaat atgttcgcca aatcctgcct gaagcgaact
      661 cacagattgt tggcttccgt tatttcaacg tttatggacc gcgtgaaggc cataaaggca
      721 gcatggcgag cgtcgctttc catctcaaca cccagcttaa taacggtgaa tcaccgaagc
      781 tgttcgaagg tagcgagaac tttaaacgtg acttcgtcta tgtgggcgat gtggcagatg
      841 taaacctgtg gttcctggaa aatggcgttt ccggtatctt caacctcggc accggtcgtg
      901 cggaatcctt ccaggcagta gcagatgcta cacttgctta tcacaagaaa ggccaaatcg
      961 aatacattcc gttcccggat aaactgaaag gccgctacca ggcgttcact caggcagatc
     1021 tgacgaatct gcgcgcggcg ggttacgata aaccgttcaa aaccgttgcc gaaggcgtaa
     1081 cggaatacat ggcctggctg aatcgtgacg cataagagct ctgcatgaaa atactggtga
     1141 tcggcccgtc ttgggttggc gacatgatga tgtcgcaaag tctctatcgc acgctccagg
     1201 cgcgctatcc ccaggcgata atcgacgtga tggcaccggc atggtgccgt ccattattat
     1261 cgcggatgcc ggaagttaac gaagcgatcc ctatgcctct cggtcacgga gcgctggaaa
     1321 tcggcgaacg ccgcaaactg ggtcatagtc tgcgtgaaaa gcgctacgac cgcgcctacg
     1381 tcttacctaa ctccttcaaa tccgcattag tgcctttctt cgcaggtatt cctcatcgca
     1441 ccggctggcg cggcgagatg cgctacggtt tactcaacga tgtacgcgtg ctcgataaag
     1501 aagcctggcc gctaatggtg gaacgctata tcgcgctggc ctatgacaaa ggcgttatgc
     1561 gtaccgcaca agatctgccg caaccattgt tatggccgca gttgcaggtg agcgaaggtg
     1621 aaaaatcata tacctgtaat caattttcgc tctcatcaga acgtccgatg attggttttt
     1681 gcccgggtgc ggagtttggt ccggcaaaac gctggccaca ctaccactat gcggagctgg
     1741 caaagcagct gattgatgaa ggttatcagg tagttctgtt tggctcggcg aaagatcatg
     1801 aggcgggcta tgagattctt gccgctttga ataccgagca gcaggcatgg tgtcggaatc
     1861 tggcgggaga aacacagctt gatcaagcgg ttatcctgat tgcagcctgt aaggccattg
     1921 tcactaacga ttctggtctg atgcacgttg cggcggcgct caatcgtccg ctggttgccc
     1981 tgtatggacc aagcagcccg gacttcacac caccgctatc ccataaagcg cgcgtgatcc
     2041 gcctgattac cggctatcac aaagtgcgta aaggtgacgc tgcggagggt tatcaccaga
     2101 gcttgatcga cattactccc cagcgcgtac tggaagaact caacgcgcta ttgttacaag
     2161 aggaagcctg acggatgcgg gttctgatcg ttaaaacatc gtcgatgggc gatgttctcc
     2221 acacgctgcc cgcactcact gatgcccagc aggcaatccc agggattaag tttgactggg
     2281 tggtggaaga agggttcgca cagattcctt cctggcacgc tgccgttgag cgagttattc
     2341 ctgtggcaat acgtcgctgg cgtaaagcct ggttctcggc tcccataaaa gctgaacgca
     2401 aagcgtttcg tgaagcgcta caagcaaaga actatgacgc agttatcgac gcccaggggc
     2461 tggtaaaaag cgcggcgctg gtgacgcgtc tggcgcatgg cgtaaagcat ggaatggact
     2521 ggcaaaccgc tcgcgaaccg ttagccagcc tgttttacaa tcgtaaacat catattgcaa
     2581 aacagcagca cgccgtagaa cgcacccgcg aactgtttgc caaaagtctg ggctatagca
     2641 aaccacaaac ccagggcgat tatgctatcg cacagcattt cctgacgaac ctgcctacag
     2701 atgctggcga atatgccgta tttcttcatg caacaacccg agatgataaa cactggccgg
     2761 aagaacactg gcgagaattg attggtttac tggctgattc aggaatacgg attaaacttc
     2821 cgtggggcgc gccgcatgag gaagaacggg cgaaacgact ggcggaagga tttgcttatg
     2881 ttgaagtatt gccgaagatg agtctggaag gcgttgcccg cgtactggcc ggggctaaat
     2941 ttgtagtgtc ggtggatacg gggttaagcc atttaacggc ggcactggat agacccaata
     3001 tcacggttta tggacctacc gatccgggat taattggtgg gtatgggaag aatcaagtag
     3061 agtgtcgttc tacaagtatg tctcttgcag atttgccagc tcaaaccgtt tttcaaaatt
     3121 taaatcttga aataataatc aataagttaa catcggagat aagatgacct caacattatt
     3181 tttctctttc gagaaaaaaa actggatagc gtactggaac agagctcttg tattcttatt
     3241 cattaccacc tattttttgg gtgggattac aaggtataaa catcttattg ttattcttat
     3301 gacaataacg acaatcgtct atctctgcaa acggccaaaa cattatctat cattatttaa
     3361 aacatttctt tttggtagtg ttgccatatt aactattgct gcattgctat cacttgttca
     3421 atccccagat gcaggtgctg gcatgaagga aattttcaaa gctatcattg aaaatacttt
     3481 gctatgcaca atagcaatac cggttatatt gagagacgag aaaagagaag atatcgaaaa
     3541 aatcgttttc ttctcattta ttagtgcgtt aggcttacgc tgtttttctg aattgattgc
     3601 ctattataag gactatcaac aagggataat gccattcgca gattatagac accgtagcat
     3661 ttctgactcg atggtttttt tattccctgc attgttaaat ctctggctta tcaaatcagc
     3721 aaaataccgc atttcttttg cggttctaag cgctattttt atttttctga tattaggaac
     3781 tttatccaga ggggcctggc tttccgtgtt agtcattgga ttaatatgga ttctgatgtt
     3841 taaacaatgg aagttactat tagtaggagt aatggtttcc atcattgcat tgtcggttat
     3901 tttcacacat aaggagatga ccgcaaagct aacgtataaa cttcaacaaa ctaatagttc
     3961 ttatcgttat gcaaatggta ctcaaggcag cgcactcgat ctaatattag aaaatcctgt
     4021 tattggttac ggttacggta acgttgcata taaagatgtc tataataaac gagtcattga
     4081 ttatccgcaa tggaccttta gacaatcgat agggccacat aattttgcgc tattcatctg
     4141 gtttggcact ggtttattag ggctggtaag tcttatgatg ctatactgtg caatactaaa
     4201 agagtgtata aaaaatgtcg tccagaataa ataccgctca cca
//
LOCUS       DAZGJT010000205         4164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZGJT010000205 DAZGJT010000000
VERSION     DAZGJT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.210"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKFK_004747"
     CDS             complement(<1..411)
                     /gene="yhaJ"
                     /locus_tag="ACHKFK_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO9054893.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGA"
     gene            516..1217
                     /locus_tag="ACHKFK_004748"
     CDS             516..1217
                     /locus_tag="ACHKFK_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO9054894.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKSLTRMQLWLDACPQRENPLIQKLALNMDKQQLIASPEGGMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGQFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKFK_004749"
     CDS             1240..1404
                     /gene="yhaL"
                     /locus_tag="ACHKFK_004749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO9054895.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(1464..2774)
                     /gene="cyuA"
                     /locus_tag="ACHKFK_004750"
     CDS             complement(1464..2774)
                     /gene="cyuA"
                     /locus_tag="ACHKFK_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO9054896.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AISDAKALLAAGKVSVKIQEPCDEILFSRAKVWNGVKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVTEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGNWGLHIGATLEKQCARGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGANDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(2802..4133)
                     /locus_tag="ACHKFK_004751"
     CDS             complement(2802..4133)
                     /locus_tag="ACHKFK_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO9054897.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKIPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
BASE COUNT         1060 a         1198 c         1000 g          906 t
ORIGIN      
        1 cgcccccgcc agcacttcgg tgatgattgc cagttgggta ttggcttttg ccgccagttt
       61 gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc tcggtcacaa tggtgagatg
      121 cgtttcccaa ccacgcgcga gggcttccgc atcggtagtc agtttatctg cggcttccag
      181 caaaacgcgc cctcgttcca gcaacatcct cccgacattg gtgaatttgg tgcgatggcc
      241 cgagcggtca aacagcacca catccagctc ttcttccagt ttttgcatgg tgtagctaag
      301 tgcggaaggc acgcgtccca gctcatccgc cgccgccgca aaactgcccc ggcgatcgat
      361 cgcatccata acccgtagtg cttccagcgt taatgccctt tctttggcca tttcgttctc
      421 attcaggaaa tttgaacata ccgggcagaa tatctggcta acaatgtagc gtccagcccc
      481 ttaccataaa aggaagtaaa gagaggtcaa gaattatgat tactacccga actgccaggc
      541 agtgtggaca agcagactac ggatggttgc aggcccggta tactttttcc tttggacact
      601 acttcgaccc gaaattgtta ggctatgcct ccctgcgcgt gcttaaccag gaagtgctgg
      661 ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt cgatatttta aatgtgattc
      721 tggatggaga agctgagtat cgcgacagcg aaggcaatca tgttcaggcc agcgctggtg
      781 aggcgttgct gctctctacc cagccgggtg tcagctatag cgaacacaat ctcagcaaag
      841 acaaatcgtt aacgcgaatg caactttggc tggatgcctg cccacaacga gagaatccgc
      901 tgattcaaaa actagcgctt aatatggaca agcagcaatt aatcgcctcg ccagaaggtg
      961 ggatgggaag cctgcaattg cgccagcaag tatggctgca tcatattgtg ctcgacaaag
     1021 gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta tttgcaatcg attcacgggc
     1081 aatttcatgc acttacgcat catgaagaga aagcggcgct gacctgcggt gatggggcgt
     1141 ttattcgtga cgaggctaac attacgctgg ttgccgattc cccactgcgc gctttgctga
     1201 tagatttgcc tgtctagttg tttttacagg gagatgatga tgagtaaaaa atcggccaaa
     1261 aagcgtcagc cagtgaagcc cgtggtggcg aaagaacctg ctcgcaccgc caaaaatttt
     1321 ggctatgaag agatgttgag cgagctggaa gccatcgtcg cggatgctga aacgcgttta
     1381 gccgaggatg aagctaccgc gtaaagacag gtgccggatg cggcgtaaac gccttatccg
     1441 gcacaatacc caaaaccggg cgtttatctg gccttgctcg ccataatctc gataatctgc
     1501 cgatccgttt gctgcatcga atggcttgct aacgcacaca ggttggcaat cgactgctca
     1561 acatcatgcg ccacaatccc ttcattgccg gtcacggcgg tatcatccag cgccattaac
     1621 accgctttcc acgcagccga agcactggtc gaaaccttca tcgcgcagct gttcgacgca
     1681 ccatcgcaaa tcatgccgct gacatcgccg atcatactgc tgatcgccat cgagatggtt
     1741 tcataacgcc catccaccag ccatgccatc ccggcggcgg cccccattgc tgcggtcgtt
     1801 gcggcacaca gcgcagacaa acgcggtaac tggttatgga tgtaaatcgc gctcaaatgc
     1861 gaaagcatca gcgcacgcgc cagccgttca tcattcgctc cgaagtgttc tgctaccacc
     1921 accacgggca ttgttgcggt gatcccctgg ttacccgagc cggagttact cattgccgga
     1981 agcgtagcgc cgcccatacg cgcatcggat gccgcgctgg tacgaatcac aatggatgaa
     2041 gagagatctt tcgccagcaa accgcgcgcg cactgttttt ccagcgtcgc accaatatgc
     2101 agcccccagt taccgctcaa accttcctgc gataacgcac aatttaactt cgcagaatcg
     2161 agaataaagc ggatcgccgc aaatgggact tcattgacga acttcaggat ctcagccagc
     2221 gtcgtcctgg aaagcaccgt cagcggcgac tcttgctcgc cctctgtcac acacgcctgc
     2281 tgggtaaaca ccacaccatt gtgcgtctca atatgcacaa tgttggtatg cccgccgacg
     2341 atggtgacac acgcccactt cacaccgttc cagactttgg cgcgtgagaa gaggatttca
     2401 tcgcaaggtt cctggatctt aactgagact ttccccgccg ccagcagtgc tttggcatcg
     2461 gaaattgcct gcgcagttgc gtctttcagc acttccagcc cggcgttagc atttccacct
     2521 aacgctccca gcgccgccgc aattggcagc cccaccattc ctgtaccggg aacggtgaca
     2581 cccagaccat tcttcatcag atttggcgaa acccaggctt ctacacgttc aaccggacct
     2641 tccagttcag ccgcagcaac cgccgccgcc agcgccagtg aaatcggttc agtacacccc
     2701 agcgccggtt ttacttcctc ctgaacggcg aggatgtaac gctgccataa cggatttaaa
     2761 gtcgaatcaa acatattaaa aaccttaaaa tttcaggtaa atcaagaaaa tgccaggaac
     2821 ggagaaacac aaagcaacaa accagtgacg ataatcaggt acagagacat tcctttgtat
     2881 ttgtgcaatg ccggtacttt gtaaaccagc cacgccggga tcaggcaccc taccatgccg
     2941 aaaatcgggc tacagataga ggtgaagctc aacaccgggg cgttcagtac gatggcgctc
     3001 caggccagca aaatggcgaa aatcatgatg ccgcgctgaa cgagattttc gttaatcttc
     3061 tcggcaggta tcttgcgacg caggatgttc attacgatcc cttgcgttgc ttcgcgaaag
     3121 cctaaataga cgccaaagaa tgcagtcatt acagcaaaga tattgagaat aacgctgacc
     3181 actttcaccc acgctgcgcc gtcaccgcta ataaactgtg cggcaatcgc cagcgcggaa
     3241 atattctgct cgtaggcttt taccgcttcg tcatgtccca tcgccagcgt gaatgacacg
     3301 gcgtagaaaa agacggtgac aaacaaaatg ccaaacgcga tattcattgc ccgcaatgct
     3361 ttatgccgcg ccacttcaat tgatttttcc cgcgaacgat aagagatcac catcgggctt
     3421 aacgtctgga taaacagaat cgacgtcagg gtaaacggca gcgtaataat ggcgtttttc
     3481 accagcagcc ccagcggcgg tagcgaaccg acgttataca gatgccacat tcccaccatc
     3541 gacacgccca gcgccgcgac caccagcagc ttggtcagca ccatgccggt cgaaattttg
     3601 aataacaatt tctcgccgcg tgaggagatc gccaccagaa tgcaaatcag taccagacca
     3661 taaaagggac tgtctgacag caacccttcc gtcacgccga aggtatgcag gtaggaagca
     3721 ctatcgttgg tgatggcggt ggaatagacg aacatccaaa tcaccagcat tacgaaatag
     3781 agtgcaccta acaggatgcc ccagttttta cctaaataac cgctgatgac gctcgggtaa
     3841 tctttacact ccggtgattc tgccagcgta ttaataaaca accgctgaaa cagatacatt
     3901 gccgggtaac caatcaccga tgagagcaaa aatacccaca atcccatcaa accgacctgc
     3961 accgggagaa aaacaatccc cgcgccaatc gccatcccga tactcataat cacccagccg
     4021 gtgtcagtac tgtcgaattt gatcgcttct cgccactcgc tctcactcat tccagcacga
     4081 cccgccgggg tcgaagcgtc tgcaatgacg cctttattcg atgcaatttc cataatttct
     4141 cgctcaatat tttgtagggc ttat
//
LOCUS       DAZGJT010000206         4056 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZGJT010000206 DAZGJT010000000
VERSION     DAZGJT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4056
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.211"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..1703
                     /gene="dpiB"
                     /locus_tag="ACHKFK_004752"
     CDS             45..1703
                     /gene="dpiB"
                     /locus_tag="ACHKFK_004752"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO9054898.1"
                     /translation="MLQLKENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSIISAVKTRDYKRLATIADKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKPGALEKGESYFITGKGSIGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMVIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1672..2352
                     /gene="dpiA"
                     /locus_tag="ACHKFK_004753"
                     /gene_synonym="citB"
     CDS             1672..2352
                     /gene="dpiA"
                     /locus_tag="ACHKFK_004753"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO9054899.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2393..3778)
                     /gene="dcuC"
                     /locus_tag="ACHKFK_004754"
     CDS             complement(2393..3778)
                     /gene="dcuC"
                     /locus_tag="ACHKFK_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO9054900.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFIRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1110 a          950 c         1043 g          953 t
ORIGIN      
        1 attacatccg tcaaagaggc tcgggacaac ccgcaaggaa aacaatgttg cagcttaaag
       61 agaataaaca gtttgcattt ttccaaagac tggcatttcc gctgcgtatc tttttgctga
      121 ttctggtgtt ctcaatattt gtcattgcag ccctggcgca atattttaca gccagttttg
      181 aggactattt aacgcttcat gtgcgcgaca tggcaatgaa tcaggcaaaa attattgctt
      241 ctaatgacag tatcatcagt gcagtgaaaa cgcgtgacta caagaggctg gcgaccatcg
      301 ctgacaaatt acaaagagat actgatttcg attatgtggt gattggcgat cggcactcga
      361 ttcgtcttta ccatcctaat ccggagaaaa ttggttatcc tatgcagttc accaaaccgg
      421 gcgcgctgga gaaaggggag agctacttca tcaccgggaa agggtcaatt ggcatggcga
      481 tgcgtgccaa aacgccaatc tttgatgacg atggaaaagt catcggcgtg gtgtcgattg
      541 gctatctggt gagtaaaatc gatagctggc gggctgagtt tttattaccg atggcaggcg
      601 tgtttgtcgt gctgttaggg attttgatgt tgctgtcgtg gttcctggcc gcgcatatcc
      661 gtcggcagat gatgggcatg gagccaaagc aaatcgctcg cgtagtccgt cagcaagagg
      721 cgctgtttag ttcggtttat gaagggctga ttgcggtgga tccgcacggg tacattaccg
      781 ccatcaatcg taacgcaaga aagatgctgg ggctgagttc ccccggacgg caatggttgg
      841 gtaaacccat tgctgaagtg gtcaggcccg ccgatttctt taccgaacag attgatgaaa
      901 aacgtcagga tgtggtggcg aactttaacg gtctgagcgt tattgccaac cgggaagcta
      961 ttcgttctgg cgatgattta ctgggggcca ttattagctt tcgtagtaaa gacgaaattt
     1021 ccaccctcaa tgcgcaactg acgcaaatta aacaatacgt cgagagcctt cgtacattgc
     1081 gacacgagca tctcaattgg atgtcgacgc tcaatggtct gttgcagatg aaagagtatg
     1141 atcgcgtgct ggcgatggtg cagggggagt ctcaggccca gcaacagctt attgacagcc
     1201 tgcgtgaggc gtttgccgat cgccaggtgg cggggctgct ttttggtaaa gtgcagcgcg
     1261 cccgcgaact ggggctaaaa atggtcatcg tccccggtag ccagctttcg caactgccgc
     1321 caggactgga cagcaccgag tttgcagcca ttgttggcaa tttacttgat aacgccttcg
     1381 aagcaagcct gcgtagcgat gaaggaaaca agatcgttga attattcctc agcgatgaag
     1441 gcgatgatgt ggtgattgaa gtcgccgatc agggctgcgg cgttccagag tctctacgag
     1501 acaaaatatt tgagcagggt gtcagtacgc gtgctgacga gcccggtgaa catggcattg
     1561 ggttgtactt gattgccagc tacgtaacgc gctgcggtgg tgttatcact ctcgaagata
     1621 atgatccctg cggtacctta ttttcaatct atattccgaa agtgaaacct aatgacagct
     1681 ccattaaccc tattgatcgt tgaggacgaa acgccgctgg cagagatgca tgcggaatat
     1741 attcgtcaca ttcccggatt cagccagata ttactggcgg gaaacctggc gcaggcccga
     1801 atgatgatcg agcgttttaa gccggggcta atcttgctcg ataactatct tcctgacggt
     1861 agagggatta atttactgca tgaactggtg caggcgcatt atcccggcga cgtggtgttt
     1921 accactgctg ccagcgatat ggaaacggtg tctgaagccg tacgttgtgg tgtatttgat
     1981 tatctcatta agcccattgc ctatgaacgg ttggggcaaa cgctaacccg tttccgccag
     2041 cgtaaacata tgctggaaag tattgatagc gccagccaga agcaaattga tgagatgttt
     2101 aatgcctatg ctcgcggtga acccaaagac gaactgccga ccggcattga ccccttgacg
     2161 ctaaacgcgg tgcgaaaact gttcaaagag cctggtgtgc aacatacggc agaaacggtg
     2221 gcgcaggcac tgaccatcag tcgcaccact gccaggcgtt atcttgaata ttgcgccagc
     2281 cgccatctga ttattgctga aattgttcac ggcaaagttg gcagaccgca acgcatatac
     2341 cacagtgggt gacatgataa agccggagga aacttgcctc cggcattact atttacttgc
     2401 ctgtgaccgc tgctgccgtt cctggcacca tcagctctgt agcaacgata acaatcacca
     2461 aaccaacaag caccggcacc gaggtgcgtt ttacgacttc aaacggcgag atcttcgcca
     2521 tcccggcaac cgcaacgact acgccagaaa cgggtgatag ggtacgaccc aggtttgacg
     2581 cctgcagcat cgggatagtc aaatacgccg ggttaatgcc ggaggagtgc gccagtttcg
     2641 ggatcatctc aacaaacgca taaaacggcg cattgcctga accggtcgtg actgccgcca
     2701 gcattgtcag gatcaccaat accagcatca ggatgatact cgccgaacca aacgaggtag
     2761 cgatagaaat cagactttga ataaagccga tggtgctaag cccctgagcg aataccccag
     2821 cggcaaccag cagcatcacc acgttagcaa atgcatctgc cataccgcga taagccactt
     2881 ccagaccaga gaaaactttc tgggtattaa agctgcggat gaactccaga atggaggcaa
     2941 ttagcataca aatcaccaga atagtgatga tgtgtaattg cggaccccat ttgccgtcaa
     3001 aaatcagcac tccgatgatc ggcgtgaacg gcaaaatggc ataaaacgca ggggcagtgg
     3061 tggtgatttc actgacatct aacatttcat gagagatgtg ctctttttta tccagataac
     3121 gttgccagaa gaagtgggcg atcgccatgc cgataattgc agcaattgag ataggcagcg
     3181 ttgttttgaa ggcgaagtca atcagcgaca tttcggaagc ctgcgccgcc agcaccacat
     3241 cccctgaagt cggtgcgaga ataatcgccg ccggggaggc acaaatggca gcagctgcgc
     3301 cacgactgat accaacgttt accatcaccg gaaacagggt tgccatcagc aaaacaccca
     3361 gaccggttgc ggaagagaca gccagtgaca tcagacaggc aacaaaatag gcggcaatca
     3421 tcagaaggta aggggagtta atatactgca atggttttga cgccagcttg accaccatat
     3481 cattcgcgcc gatatgggtc atgtaagcgg caaagccaca cagcatcata atcatcatgc
     3541 cgaggtcgcc gccgcggctc attagcaata ttttaacgta ttcaacgata tccgtggcgc
     3601 tgtagcctgt tgaagcctgg ctggacggta acactttgtg ccccataatg gcactgataa
     3661 tcagcaataa caggccaccg acaaataaca cgccagtggc agaataccct ttaatgatgt
     3721 agcgagctac acccacaata accacaaccc caataaggag ttcaatgaat gtcagcataa
     3781 tttttcctgt ctccaggccc caaagtaaat aataaaaaat tcctaaaagt taaggaaaaa
     3841 atatgcctaa taaattggcg gtgaatgctg attaaaatca agaaaaactg ccattaagac
     3901 attgaagttg ctgtttttat acgtagataa caaaacgcat atttagcggt tggcaaatat
     3961 cacataataa tgtgtgtaaa ttgatggtgg attgatgcaa atttgttaat tgtaaggtgt
     4021 tagctgcggt tgtgtgctca aaaaataatc ttccat
//
LOCUS       DAZGJT010000207         3919 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZGJT010000207 DAZGJT010000000
VERSION     DAZGJT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.212"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(116..835)
                     /gene="dcuR"
                     /locus_tag="ACHKFK_004755"
     CDS             complement(116..835)
                     /gene="dcuR"
                     /locus_tag="ACHKFK_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO9054901.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDSPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAVTIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(832..2463)
                     /locus_tag="ACHKFK_004756"
     CDS             complement(832..2463)
                     /locus_tag="ACHKFK_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO9054902.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MHSLRYSHPEAQRIGQPFKGDDILKALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVNVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVFAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2644..2874
                     /gene="yjdI"
                     /locus_tag="ACHKFK_004757"
     CDS             2644..2874
                     /gene="yjdI"
                     /locus_tag="ACHKFK_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO9054903.1"
                     /translation="MDRALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            2886..3158
                     /locus_tag="ACHKFK_004758"
     CDS             2886..3158
                     /locus_tag="ACHKFK_004758"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9054904.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3387..3683
                     /gene="ghoS"
                     /locus_tag="ACHKFK_004759"
     CDS             3387..3683
                     /gene="ghoS"
                     /locus_tag="ACHKFK_004759"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO9054905.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLSIMTVDDYFRQFYK"
     gene            3711..3884
                     /gene="ghoT"
                     /locus_tag="ACHKFK_004760"
     CDS             3711..3884
                     /gene="ghoT"
                     /locus_tag="ACHKFK_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO9054906.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
BASE COUNT         1025 a          872 c          947 g         1075 t
ORIGIN      
        1 tggagggata attatatttg atctggcaca agttttactg atgaacgatg taacttgtgc
       61 cagggggatt ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt
      121 ggcaatattg tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac
      181 gccccgtgac gccataatgg atactggtga acaatatgtg gcagttgacc agccagataa
      241 ggtatttacg gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa
      301 actcatagtc ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta
      361 agccttttgg caagcgacga ggatcctgct cgttggagct gctgccgtgg attagctgat
      421 caagctcggc ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc
      481 cggtgagcgc ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacaacac
      541 cgtaatgcag cgaatcttta atggttactg catcggcagc ggaggagatg acaatcacat
      601 cacttttgca acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca
      661 tatagatatc tagcaatatc aggtcgatag gcgaatcgct attgaagata atctctttgg
      721 ctttctccag cgtcgaagcc gttccacagc attgaaagcc cgggatttgt gccacgtatc
      781 ggcgattcag ctccgcgacc attgcgtcat catcgataat taatacattg atcatctgtt
      841 cgacctctcc ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga
      901 ttccacggcg atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac
      961 gcctcgctcg cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg
     1021 tgcgatcccc ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa
     1081 tgttacgcta atttcgccac cgggttccgg ccctaatgcc tccagcgcgt tttctatcag
     1141 atttcccaac gtggtaatca gcgtcgcgac ctgatcctca ctaccgctgt ctggcagctg
     1201 gctttcactg tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat
     1261 taaaaaacca gcgaaaaccg gagatttgat cttacccagc agagagccaa tctcttcctg
     1321 atagttattg gctgttttga gaatgtaatc ttccaactgc ttataactct tcagatgcaa
     1381 taatccgaga atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcgtc
     1441 agcatagttg accagaccgt cgagtcgctg catcagttta cgtacttcag ttttgtccct
     1501 gaaggttgaa atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag
     1561 taatagtcgg tccttaatcg taatctcttc gtcgcggcgt ggggttccgt cgcgtaacac
     1621 ttccgagaca tctaccacct gtgaccacga gtggcttagc gtcgacagtt tctcatcgtc
     1681 ctgcgactta cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc
     1741 gcgatcgtcc acggcaacga cgccttcttt gatggactgc aacatggcct ggcgttgctc
     1801 aaacagtgtg gagatttcgt agggttccag gccgaaaagg atttttttca gtacgttaac
     1861 cagaatgcat gtgccgatca gtccgaccag catgccaaat agtaccgacc agataatgct
     1921 ccagcgactg tcattgatct gttgggtcac gcggcttaac tcaaggccga tcgccaccac
     1981 gccaatttgt ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag
     2041 aaaaccgcga ttgatagcga cattttcttc gccattcagc gctttaagga tgtcatcacc
     2101 tttaaatggc tgaccaatac gctgggcttc aggatgcgag tagcgaagac tgtgcatatc
     2161 ggtaacgaca ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat
     2221 tccactctcc tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt
     2281 acgcgctact gccagagcct tgttagctag cccgtctcgc gtcatatcac tgatttgcga
     2341 gaagtaaatc agatgcacca ccaatagcac cgagaacagt accgcgctga ccattaagat
     2401 cacagtggta ctcaatttca tcggacgttt gcgtaacata cggtagggca atgaatgtct
     2461 catcagcttc cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca
     2521 aagggagtaa gaatgattgg ctatataggg gaagagattc tggcaacgga aactgccagt
     2581 gctgtatgaa gattccgggg ctatgcttat agcgataatc atactgatga gagagggaag
     2641 gtcatggatc gggcgctact ggacgggggt tatcgctgtt ataccggcga aaagatcgat
     2701 gtctatttca atactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag
     2761 ttatttaatc tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt tgccactgtg
     2821 gttaaagtga ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg
     2881 gtaaaatgga aatacgcgaa ggccacaata aattttacat taatgacgaa caaggcaagc
     2941 aaatcgctga aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg
     3001 atgtcgatga aagcctgaaa gggcaaggga ttggtaaaca gctggttgcg aaagtcgtgg
     3061 aaaaaatgcg tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat
     3121 ttgataaaac acgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat
     3181 atttttcatg ctctccgcgg tgtgatgcag gagagcatct gaagggtagg gggatgcaca
     3241 aagaatggtc agagagagcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa
     3301 aaagaatatc tcctatatga gaatcatcat tcggggttaa taagttttgc gtccccagag
     3361 cgttcaatat tgataggagt catattatgg aaggtaaaaa caagttcaat acttatgttg
     3421 tttcttttga ttatccatca tcttattcct cagtgttctt aaggttaaga tcattgatgt
     3481 atgatatgaa tttctcctct atcgtggctg atgaatatgg gataccacga caactgaatg
     3541 aaaactcctt cgcaataacg acatcgttag ccgcaagtga aatcgaagat ttaatcaggc
     3601 tcaaatgctt agacttaccg gatattgatt ttgacctcag cattatgaca gttgatgact
     3661 atttccgtca gttttacaag tagccaggaa ggataagaag aacataaaaa atggcactat
     3721 tctctaaaat attaattttt tatgtgattg gtgtgaacat atcctttgtc attatctggt
     3781 ttatctcaca tgagaaaaca catattcgtt tacttagtgc atttctggtc ggaataacct
     3841 ggccaatgag tctgcctgtg gcattacttt tttctctctt ttaggagctg tagttgagtg
     3901 gacccgctgg ttatcttga
//
LOCUS       DAZGJT010000208         3918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZGJT010000208 DAZGJT010000000
VERSION     DAZGJT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.213"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..3434
                     /locus_tag="ACHKFK_004761"
     CDS             <1..3434
                     /locus_tag="ACHKFK_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
                     /protein_id="HFO9054907.1"
                     /translation="VRETIRTLGQRVREGYTPREDGAGASSQYSRSGKPDPFKVPEGE
                     GERYRDDLARMMKSLRSGAMTANIGRTPPVLRHLGAPDLPLVISRDTVRKATNGVKHV
                     VPMDVIERLPELMHDPDAIYRSATERNAVVMLLDAVDKNGDPVVSAVHMKATDKRIEI
                     NKVASVYGTKGGMNKANSLDKAGLTLYRKEKLSRDNPQYSGLQLPKEERSYRGSVDKI
                     LYPEDIRKGPYYSRTSSLTPEETIASRFVRQMQDKFQVLKAVQENIRKTGGKIDDSNN
                     AYMAEELFHGKAENDLNVMKERYVQPLAKLLADYKIAQADLDEYLYARHAPERNAHIA
                     KINPKMPDGGSGMTNAEAAEIMQRVRNSGKQAQYDRLAGIVDDMLARRRELIREAGLE
                     ESGVVDAWQNAYRYYVPLKGQDIDGVVSLPRTGKGFTIGGRESWQAMGRASRAQSPST
                     QAIQDLSESLIRHRKNEVGNAFLKLVQDNPDKDYWQVFTDDRPDTMRVIAERKDQETG
                     ETIREVVERPVAMAMMADRYFTTKKDGKTYYIKLHDPRLMRAMKNMGPETSNAVIRTL
                     GKVNRFLATVNTSYNPEFLVSNFIRDVQTAVMNLKAEQGRSDGKLKELDNLSALAVVK
                     DSRSAMSAVYASLRGKTLTGKGAQWQKVWKEFVEDGGKTGWFNMGDLEGQQKEMDRLV
                     SLAKGGWKGQSIGAWHSFLNLVEDANGAVENALRLSAYKHARDAGLSRQQAASLAKNM
                     TVNFNRRGEQGMLMNSLYMFANASIQGTANLVRTLGHLNGDGPLPERLRWKNLNVPQK
                     IVLAAVGAGYLLGSLNRSVAGEDDDGVNWYDKVPSHVKERNLVIMKSMFGGKAGEYWS
                     IPLPYGYNVFFLLGHTAEGVAAGDLTASRAAGNVVGGILGAFSPIGSETSETLSGALL
                     KNAAPTILRPFANLAMNENFMGAQIYQENMPFGTPKPDSQLGRRSTPEAYKAFASWLN
                     AFSGGSQYRPGAVDITPESLKYWVDYISGGTGRFISKTTDAAVKSLNGIDIPEQQVPF
                     LGKISGEVMPYADQQKMYDRMTEVAQYHAELKSLTGAERTAFIDENNGKLSMNGLMQD
                     TRKRLKDLRKQRDAIYADSTLSLAQQSAMVKSVERDMKIAVDRFNREYNKKVGVE"
     gene            complement(3656..>3918)
                     /locus_tag="ACHKFK_004762"
     CDS             complement(3656..>3918)
                     /locus_tag="ACHKFK_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054908.1"
                     /translation="LGNTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGG
                     TFIFALMSHAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
BASE COUNT         1001 a          905 c         1160 g          852 t
ORIGIN      
        1 ttgttcgcga aactattcgc acgttaggcc agcgtgtgcg ggaaggttac acgccgcgtg
       61 aggatggcgc gggcgcatcg tctcagtact cccgtagtgg taaacctgat ccgttcaaag
      121 tgccggaagg tgagggcgag cgttatcgtg atgaccttgc cagaatgatg aagtcgttac
      181 gttctggtgc aatgactgca aacatcgggc gtacgccgcc ggtattgcgc caccttggcg
      241 caccagattt gccgctggtt atctcccgcg acactgtgcg gaaggcaacc aatggagtga
      301 aacatgtggt gccaatggat gttatcgaga gacttcctga gctgatgcac gatccggatg
      361 caatttaccg ttccgcgaca gaaagaaatg cggttgtgat gctgcttgat gccgtggata
      421 aaaatggtga tccggtggtg tcggcagtgc acatgaaggc aacagataaa cgaatagaaa
      481 ttaacaaggt ggcttctgta tatggaacaa aaggggggat gaacaaagct aatagcctgg
      541 ataaagcagg tttgacgctt taccggaagg aaaaattaag ccgcgataac cctcagtaca
      601 gtgggcttca attgcccaaa gaggagcgtt cttatcgcgg ctctgtagat aaaatactct
      661 atcctgaaga tattcgcaag gggccgtatt actcccgtac cagcagtctg acaccggaag
      721 agacaattgc atcgcgtttt gtgcgtcaga tgcaggataa attccaggtg ctgaaagctg
      781 ttcaggagaa catccgtaaa actggcggaa aaatagacga cagtaacaac gcttatatgg
      841 cggaagaact cttccatggg aaggcggaaa acgacctgaa cgtgatgaag gagcgctacg
      901 ttcagccact ggctaaatta ctggcggact acaaaattgc gcaggccgat ctggatgagt
      961 acctctacgc ccgtcacgcg ccggaacgta acgcgcatat cgcgaaaatc aacccgaaaa
     1021 tgccggacgg cggttcgggg atgaccaacg cggaggcggc ggaaatcatg cagcgtgtgc
     1081 gtaacagtgg aaaacaggca cagtatgacc gtctggcagg gattgttgac gatatgctgg
     1141 cccgtcgccg tgagcttatc cgtgaggccg gacttgaaga gagtggtgtg gtggatgcct
     1201 ggcagaacgc ctaccgttac tacgttcccc tgaaaggtca ggatattgac ggtgtggtgt
     1261 cactgccccg tacaggtaag ggcttcacta tcggcggacg tgaaagctgg caggccatgg
     1321 ggcgtgcatc ccgtgcacag tctccgtcca ctcaggcgat acaggacctg agcgaatcgc
     1381 tgatccgcca tcgcaaaaac gaagtgggta acgccttcct gaaactggta caggataatc
     1441 ccgacaagga ttactggcag gtattcactg atgacagacc ggataccatg cgtgtgattg
     1501 cagagcgcaa ggaccaggaa actggtgaaa ccattcgcga agttgtcgag cgtccggtgg
     1561 cgatggcaat gatggcggat cgctacttca ccaccaaaaa ggacggcaaa acttactaca
     1621 ttaaacttca tgatccgcgc ctgatgcgtg cgatgaagaa catggggccg gaaaccagta
     1681 acgccgtaat ccgtacgctg gggaaagtta accgcttcct ggcaacggtg aacacgtcgt
     1741 ataacccgga atttctggtc agtaacttca tccgtgacgt gcagactgcg gtgatgaacc
     1801 tgaaggctga gcagggaagg agcgacggta aactgaaaga gctggataac ttatccgcgc
     1861 tggctgtggt gaaagacagc cgttccgcca tgtcagccgt atacgccagt ctgcggggta
     1921 aaaccctcac gggcaaaggc gcacagtggc agaaagtgtg gaaagagttt gttgaggacg
     1981 gaggtaaaac cggctggttt aacatgggtg accttgaggg ccagcagaag gaaatggatc
     2041 gccttgtgtc gctggcgaag ggcggatgga aaggccagag tatcggtgca tggcattcgt
     2101 tcctgaacct ggtcgaggat gccaacggcg cggttgaaaa cgctctgcgt ctttctgcct
     2161 ataaacacgc ccgtgatgcc ggtttgtcac gccagcaggc ggcgtctctt gccaaaaaca
     2221 tgacggtgaa ctttaaccgt cgtggtgagc aggggatgct gatgaactcg ctgtacatgt
     2281 ttgccaacgc cagcattcag gggaccgcaa acctggtgag aacgctcgga catcttaatg
     2341 gcgacgggcc gttaccggag cgccttcgct ggaagaatct caatgtaccg cagaaaatcg
     2401 tgcttgcagc tgtgggagcg ggttatctgc ttggctcgct taaccgcagt gtggcgggtg
     2461 aggatgatga cggggttaac tggtacgaca aagtaccgtc tcatgtgaaa gagcgtaacc
     2521 tcgtcatcat gaaatcgatg ttcgggggca aggccggtga gtactggagt attcctctgc
     2581 cttacgggta caacgtgttc ttcctgcttg gtcatacagc tgaaggtgtg gcagcgggcg
     2641 acctgactgc ttcacgtgct gccggtaatg ttgtcggcgg tatcctgggg gcattcagcc
     2701 cgatcggcag cgagacgtcg gaaacgttgt cgggggcatt gctgaaaaac gcagcaccga
     2761 ccattctgcg tccgtttgcg aaccttgcca tgaatgaaaa cttcatgggg gcgcagattt
     2821 accaggagaa catgccgttt ggtacaccaa aacctgacag ccagctggga agacgttcaa
     2881 cgccagaagc gtacaaggcg tttgcatcct ggctgaatgc gttctcaggt ggcagccagt
     2941 accgtcccgg cgcggtggat atcacaccgg aatcgctgaa atactgggtg gactatatct
     3001 ccggagggac agggcgcttc atttccaaaa ccacggatgc ggcggtgaaa tcgctgaatg
     3061 gtattgatat accggagcag caggtgccct tcctggggaa aatttcaggt gaggtgatgc
     3121 cgtatgctga ccagcagaag atgtacgacc ggatgacaga ggttgcgcag tatcacgcag
     3181 agctgaagag tctgaccggt gcagaaagaa cggcgttcat tgacgagaac aacggaaaat
     3241 tgtcgatgaa cgggcttatg caggataccc ggaagagact gaaggatttg cgtaaacagc
     3301 gtgatgccat ttacgccgac agtactctca gtctggcgca acagtcggcg atggtgaaat
     3361 cggttgagcg ggatatgaaa attgccgtgg atcggtttaa ccgcgagtac aacaaaaaag
     3421 tgggagtgga gtaaagaaca tgccccgtac ggaagtgcgg ggcatgctta acgggatgtg
     3481 tacataaatg ggtacggata tgacgattat tttggtgctt tctgtattta aatcaattag
     3541 ttaatacttg tattcattat atccatttaa ctaagaggac aatgcggcac gagtataccc
     3601 gctaatggac tacggagtaa gtacacagcc gctcgattgc ttaaaccctc gccatttatg
     3661 ccgggttttt atcatttttc ttaatgtttt ccgcacgttc tgctttttga cgtgcttctg
     3721 ctttgcgctt gttgctcatg tcgttacgaa tctgtgcatg actcattaac gcaaagataa
     3781 aggtgccgcc gcagatgttc cccgctaaag taggtagtgc aaagggccag atgaaatcgc
     3841 tccagtgcag cgtgccgtta aacaccagat agaggatttc aacagaaccg acaacgatgt
     3901 gggtggtgtt acccaggg
//
LOCUS       DAZGJT010000209         3915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZGJT010000209 DAZGJT010000000
VERSION     DAZGJT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.214"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            276..794
                     /locus_tag="ACHKFK_004763"
     CDS             276..794
                     /locus_tag="ACHKFK_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO9054909.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNAQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            810..1349
                     /locus_tag="ACHKFK_004764"
     CDS             810..1349
                     /locus_tag="ACHKFK_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO9054910.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1569..2051)
                     /locus_tag="ACHKFK_004765"
     CDS             complement(1569..2051)
                     /locus_tag="ACHKFK_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFO9054911.1"
                     /translation="MIWAFAKAYWKQLVIMAMLAVLVISGVVAWNAHGSRQYDAGYAQ
                     AKKDRKAEDEIVRQHYEQEKSINEREAQQRIDQARNDALDAAARAGRLQQQLVAIREQ
                     LRHYNAIVGAGSSAADTGVLLSDVFSKSLERNRQLAEYADRASEAGRVCEKQYDTLTR
                     "
     gene            complement(2048..2677)
                     /locus_tag="ACHKFK_004766"
     CDS             complement(2048..2677)
                     /locus_tag="ACHKFK_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 19 protein"
                     /protein_id="HFO9054912.1"
                     /translation="MEIKQFQRAAGISEALAARWFLHITAAMNEFGIIKPDDQAMFIA
                     QVGHESGGFTRLQENFNYSVNGLSGFIRAGRITPDQASTLGRKAYEKSLPLERQRAIA
                     NLVYSKRMGNNAPGDGWNYRGRGLIQITGLNNYRDCGNGLKVDLLESPELLAQDEYAA
                     RSAAWFFASKGCMKYTGDIARVTLIINGGRNGIDDRRARYITASKVLAV"
     gene            complement(2667..2975)
                     /locus_tag="ACHKFK_004767"
     CDS             complement(2667..2975)
                     /locus_tag="ACHKFK_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO9054913.1"
                     /translation="MATYELFFLITNAVICTGIAIRVVTFRRNGSQHRRWGGWLAYFL
                     IVAAASIPVRVAYAIWLRTPMAVDLSEVIINAVMLAAVIKTRGNVVQIFKVSRSKHGD
                     "
     gene            complement(2962..3366)
                     /locus_tag="ACHKFK_004768"
     CDS             complement(2962..3366)
                     /locus_tag="ACHKFK_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin"
                     /protein_id="HFO9054914.1"
                     /translation="MSVQLTSETLNQWLSLGSLAAVIAGVPPEVALGSLSGAVIFVTS
                     AVEYPISRRVLLSMLSFLCGLLFYKPAASILIGVASLIPTITQDSFEKGVVFSAGAFV
                     SAIVAVRIGIWLYHRSDNPRELIPGRKDDGNV"
BASE COUNT         1092 a          936 c          890 g          997 t
ORIGIN      
        1 acctcgctgt cggatgcttt tgctctttga gattatcaaa gcggcagata ttctttcatc
       61 ttaaatttta cgtctttgtt ctgactgaag ttcatcctgt tcggctgcga aataaataca
      121 tcattaatac atatgttgtg atgatgtatt tttataaatt atttcccgca tgaattttaa
      181 taaatttaat ctattccttt atacgcaata catttacttt cctcttttga tgatcttaaa
      241 tgtctcattt ttcgtaatgt gtataacaag gaatagtgat gaaatttaaa aaatgtcttc
      301 tgcctgtggc aatgttagcg tcattcactc tggcaggatg ccagtcaaat gctgatgatc
      361 atgccgccga tgtttatcaa accgatcaac tgaataccaa acaagaaact aaaaccgtta
      421 atattatttc cattcttccc gcaaaagttg ccgtagacaa cgcccaaaat aaacggaacg
      481 cacaagcctt cggcgcgctt attggcgctg tcgctggcgg tgttatcggc cacaacgtcg
      541 ggtctggcag caattccgga acgacggcag gtgcagttgg cggcggagct gtaggcgcgg
      601 cagcgggttc gatggtgaat gataaaacct tagtggaagg tgtttcttta acatataagg
      661 aaggcaccaa agtgtatacc tccacccagg tgggtaaaga gtgccagttt acgacaggtt
      721 tagccgttgt tattaccacg acgtataacg aaacgcgtat tcagccaaat accaaatgtc
      781 ctgaaaagag ctaataatca ggaggagtca tgaagaaagt ttttctttgc gccatcttag
      841 cctccttaag ctatccggct atcgcctcat cattgcagga tcaactctcg gctgtcgcag
      901 aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca tgacgaatgg gtcgcggagc
      961 gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa cgcccaggcc gcggctaata
     1021 aaagagcggc aacggcagca gcgaataaga aagctcgtca ggataaactg gacgccgaag
     1081 ccactgcgga caaaaaacgc gatcaaagtt atgaagatga gctacgtagc ttagagattc
     1141 agaaacaaaa actggcgctg gcgaaagaag aagcccgcgt caagcgcgaa aacgaattta
     1201 tcgatcagga actgaagcac aaagctgcgc aaaccgatgt ggtgcaatct gaagctgacg
     1261 caaacagaaa tatgactgaa ggcggtcgcg atctgatgaa aagcgtgggc aaagcagaag
     1321 agaacaaatc ggacagctgg tttaattaat cgatgtaagt aacttcaagc ctataattct
     1381 tgaagataaa aaaccctctg tagtaacaga gggttttgtt cattcatagt gcagggatca
     1441 aaatcattcc cactcaatta tttacgataa ccataaccaa ttgagtgata acatttttcc
     1501 aaatctcaat ttttcccgta ccgttttata taccgtcacc ggaaatcagt accgtgaaaa
     1561 atgccatgct atctggtcag ggtgtcgtac tgtttttcgc agactcttcc ggcttcggat
     1621 gcccggtcag catactctgc cagttgtcta tttctctcga gagatttgct gaacacgtcg
     1681 gaaagcaaaa ctccggtgtc tgcggctgac gacccagcgc cgacaatggc gttatagtgc
     1741 ctgagctgct cacggatggc aacgagctgt tgctgcaacc ggccagcgcg agcggcagca
     1801 tcaagagcat cattgcgcgc ctggtcgatc ctctgctgcg cttcacgttc attgatcgat
     1861 ttctcctgtt cgtagtgctg acgaactatc tcatcttcag ctttgcggtc tttcttcgcc
     1921 tgcgcatacc cggcatcgta ctgacgactg ccgtgtgcat tccaggctac aactcctgat
     1981 atgaccagaa cagcaagcat cgccatgata accaactgtt tccagtatgc ttttgcgaat
     2041 gcccagatca taccgccagc accttactgg cagtgatgta ccgcgcgcgc cggtcgtcga
     2101 tgccgttccg gccaccattg ataatcagag ttacacgtgc aatatcgccg gtatacttca
     2161 tgcatccttt gctggcgaag aaccacgccg cgctacgagc cgcatattcg tcctgcgcca
     2221 gcagttcagg gctctccagc aggtcaacct tcaggccgtt tccgcaatca cgatagttat
     2281 tcaaaccggt aatctggata agtccgcgcc ctcggtaatt ccagccatca ccaggggcat
     2341 tgttccccat gcgtttgctg tacaccagat tggcgatcgc acgctggcgc tccagaggca
     2401 gagacttctc atatgctttt cgtcccagcg tgctggcctg gtccggcgtg atgcgtccgg
     2461 cacggataaa cccggacagt ccattaacgc tgtagttgaa gttttcctgt aaccgggtaa
     2521 atcctccgga ctcatgcccg acctgcgcga taaacatcgc ctggtcatct ggtttgataa
     2581 tgccaaactc attcatggca gcagtgatat gcagaaacca gcgtgcggcc agcgcctcgc
     2641 taataccagc agctcgctgg aattgtttaa tctccatgtt tagacctcga tactttgaaa
     2701 atttgaacga cgttaccgcg cgttttaata accgcagcga gcatgacagc gttgataatg
     2761 acctcagata aatccacagc cattggcgtg cgtaaccaga ttgcataggc gacacgaaca
     2821 ggaatactgg ccgcagcaac aatcaggaaa taagcaagcc accctcccca tcttcggtgt
     2881 tgtgacccgt tacgccgaaa tgtgacaacg cgaattgcta tgccagtaca aataactgca
     2941 ttggtgataa gaaaaaaaag ctcatacgtt gccatcgtct tttctccccg gaattaactc
     3001 acgtggatta tcggaacggt gatagagcca gatgccaatt cgtactgcga cgattgctga
     3061 cacgaatgcg ccagcagaga aaacaacccc tttctcgaaa gagtcctgcg tgatagtagg
     3121 gatcaggcta gctacgccta taagaattga tgctgctggt ttgtagaaaa gaagcccaca
     3181 gagaaagcta agcatcgaca agagtacgcg acgactaatt gggtactcta ccgcagaggt
     3241 aacaaaaatc accgctcctg acaaagaccc caacgctact tctggaggta ctcctgctat
     3301 caccgcagct agagaaccaa gactaagcca ttgatttaat gtttcactgg ttagttgaac
     3361 tgacatagaa accaccgttt actgtgcata aagacccacc ttagttggtg agtccatcat
     3421 acacaattaa tacatatatg gtttaattga aatccccaaa aacattctga ataggtgagg
     3481 tgttttctgg ctccatagct atgacgtggc ttttgatgcc taactttata aggtgccacg
     3541 acaatgtgat aggtacatag gattcagtta ccaatgcaaa gctttgtgtg tctcagtttt
     3601 gtctcatgcc aacccagtag aatcaatcta cttaatctct ctgcagtgaa tattttgttt
     3661 gcacgcaatt gcacatgatg aactggcgct tatgataatt tctgacggtg atctgatggg
     3721 agtgaaagtg cggctatctg atgatacaat gctcttcaaa ataaacagtt aggtgatttt
     3781 tatgttggtc aaagatgcgc taaaaaggga taacaataac cttgacttaa tacgtatttt
     3841 ttgtgcaatt tctgtaatat ttgcccattc atattatctt gcaaattctg atggagccca
     3901 ggagccggtt cagtc
//
LOCUS       DAZGJT010000210         3891 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZGJT010000210 DAZGJT010000000
VERSION     DAZGJT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.215"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..2327
                     /locus_tag="ACHKFK_004769"
     CDS             66..2327
                     /locus_tag="ACHKFK_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO9054915.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSLPEVIGNGW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTH
                     FGAGDITELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            2396..3571
                     /gene="senB"
                     /locus_tag="ACHKFK_004770"
     CDS             2396..3571
                     /gene="senB"
                     /locus_tag="ACHKFK_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HFO9054916.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
BASE COUNT         1105 a          903 c          915 g          968 t
ORIGIN      
        1 gtagaacata attaataaac aaatagttat acttatcttt attaatatta tacccaagga
       61 aatttatgaa cgttataaaa ctggctatcg gctcaggcat attattgctc agctgcggtg
      121 cttactcaca atccatcagt gaaaaaacta attccgacaa aaaaggagcg gcagaattca
      181 gtccgctcag cgtttctgtc gggaagacga ccagtgagca ggaagctctc gagaaaacag
      241 gcgcgaccag ttcccggaca acggacaaaa acctgcaatc acttgacgca acagtgcgta
      301 gtatgcctgg tacttatact caaatagatc ctggtcaggg agcaatcagt gtgaatattc
      361 gaggcatgag cggatttggt cgtgtaaaca ctatggtcga tggtattacc cagagttttt
      421 acggaacctc tacctccgga acaacgacgc atggttcaac taacaatatg gctggcgtac
      481 ttatagatcc taacttactg gtagcagttg atgttacacg cggtgacagc agtggctctg
      541 aagggatcaa cgcccttgcc ggtagtgcaa atatgcgtac tattggcgtt gacgatgtaa
      601 tatttaacgg taatacatat ggccttcgtt cacgtttctc tgtcggtagt aatgggctgg
      661 gacgcagcgg aatgatcgcc cttggtggaa aaagcgacgc ttttacggat acgggaagca
      721 ttggcgttat ggctgctgtg agcggcagtt ctgtgtactc taatttctca aatggttctg
      781 gaattaacag caaagagttt ggttatgata aatatatgaa gcagaacccc aaatcccaac
      841 tgtataaaat ggatatcaga ccagacgaat ttaacagctt cgaactttcc gctcgaacct
      901 atgaaaataa atttacacgt cgtgatataa ccagtgacga ctattacatt aaatatcatt
      961 acaccccttt ttctgaatta attgacttta acgtaacggc cagtaccagt cgcggtaatc
     1021 aaaagtatcg tgatggctcg ctgtatactt tctacaaaac ctcagcgcaa aatcgttctg
     1081 acgcgctgga tatcaacaat accagccggt tcactgtcgc ggacaatgac ctggagttta
     1141 tgctgggcag caaactgatg cgtacccgct atgaccggac cattcactca gcggcgggtg
     1201 acccgaaagc gaatcaggaa tcgatcgaga acaatccgtt cgcaccctcc ggccagcagg
     1261 atatttcagc gctgtatacc gggctgaagg ttacgcgcgg catctgggag gcagatttca
     1321 atctcaacta cacacgtaac aggatcacag ggtacaagcc cgcctgcgat tcacgcgtta
     1381 tctgcgtgcc acagggtagc tacgatattg acgataaaga gggtggcttc aacccttcag
     1441 ttcagctttc tgctcaggta acaccatggc ttcagccgtt cattggctac agcaaatcca
     1501 tgcgcgcccc gaacatccag gagatgttct tctctaattc aggaggcgca tccatgaacc
     1561 cattcctgaa gcctgaacgt gcagaaacct ggcaggcggg ttttaacatt gataccagag
     1621 atttactggt cgaacaggat gccctgcgct ttaaggctct ggcgtaccgc agcaggatcc
     1681 agaactacat ctacagcgag tcttatctgg tttgttctgg aggtcgtaaa tgcagtctgc
     1741 ctgaggtgat tggcaatggc tgggagggca ttagcgatga atacagcgac aatatgtaca
     1801 tctacgttaa ctcggcaagc gacgttatcg caaagggctt cgaactggag atggattatg
     1861 atgcaggttt tgcttttggc cgactctctt tcagccagca gcaaacagac cagccaacct
     1921 ccatcgccag cacccacttt ggcgcagggg atataaccga actgcccaga aaatacatga
     1981 cgctggatac tggtgttcgc ttcttcgata acgcgttgac cctgggcact atcataaaat
     2041 acacaggcaa ggctcgtcgc ctgtcgcctg attttgagca ggacgaacat accggcgcaa
     2101 taatcaaaca ggatttgccg cagatcccaa cgattatcga tctctatggt acttacgagt
     2161 acaaccgcaa cctgacactg aaactttcgg tacaaaacct gatgaacaga gattattcgg
     2221 aggcgctgaa taagctcaac atgatgccag gtcttggtga cgagacccac ccagccaatt
     2281 ccgcgcgtgg cagaacatgg atatttggcg gggacattcg tttctgatga caatttccgc
     2341 taggttcaga caatacgttt tctctcttat gtcaatttta ttgcaggaac gaaaaatgaa
     2401 tattttcact ttatccaaag caccgctata cctgttaatt tcactatttt tacccacgat
     2461 ggccatggct atcgatccac ctgaacgcga actttcgcga tttgccctga aaacgaatta
     2521 ccttcagtcc cctgatgaag gcgtctatga actggcgttt gataatgcca gtaaaaaggt
     2581 gtttgcagca gtcaccgatc gtgtaaatcg tgaagccaat aaaggctatc tgtattcgtt
     2641 taattcagat tcgctgaaag tcgaaaataa atacacgatg ccataccggg cattttcgct
     2701 ggcgataaat caggataaac atcagctcta tatcggacac acccagtcag cgtccctgcg
     2761 tatcagtatg tttgacaccc caaccggcaa actggtaaga accagcgaca ggttaagttt
     2821 taaagcggca aacgctgcag attcgcgttt tgagcatttt cgccatatgg tttacagcca
     2881 ggattccgat accctgtttg tgagttatag caatatgctg aaaacggccg agggcatgaa
     2941 gcctctgcat aagctgttaa tgctcgacgg gacgacgctt gccttaaaag gcgaggttaa
     3001 ggatgcttac aaaggtacag cgtatggtct gacgatggat gaaaaaacac agaaaatcta
     3061 cgttggcgga agagattaca tcaacgaaat tgatgcgaaa aatcagacgc tgctgcgtac
     3121 catcccgttg aaagatccga gaccacaaat cacaagtgtg cagaatctgg cggtggactc
     3181 cgcttctgac cgtgcctttg tggtggtatt cgaccatgac gatcgttccg gtacaaaaga
     3241 tggactctat atttttgact tacgcgacgg taaacagctt ggctatgtgc acacaggagc
     3301 cggagctaac gcggtgaaat acaatccgaa atataacgaa ctgtatgtca ccaacttcac
     3361 tagcggcacc atcagcgtag tggatgccac caaatacagc atcacccgtg aatttaacat
     3421 gccggtctac ccaaaccaga tggtgttgtc ggacgatatg gatacccttt acattggcat
     3481 caaagaaggc tttaaccgcg attgggatcc tgatgtgttt gtggaaggag ctaaagaacg
     3541 tattctgagc attgatttga aaaagtcgtg atcgggggga aaaaacatgg gataaaaact
     3601 ccgttattcc actaaactgg ccggactagg aaaagcaaac atctctgtcc tgccggtacg
     3661 cctgttacgc cgctccatga ggctgaggaa tctccagaaa acgagacagg cacagcattg
     3721 tacgatcaac tgatttgtgc caataattaa atgaaatcac agctatgcct gttcatgggg
     3781 ttctaggccg gaaccccaga tttttccgat tgtttgtttt ctaactgatt attcagtagt
     3841 tttagagggc tgacgctcag tggaacgaaa actcacgtta agaggttttg a
//
LOCUS       DAZGJT010000211         3877 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZGJT010000211 DAZGJT010000000
VERSION     DAZGJT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.216"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..3435
                     /locus_tag="ACHKFK_004771"
                     /pseudo
     CDS             1..3435
                     /locus_tag="ACHKFK_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="LPD38 domain-containing protein"
     gene            3586..>3877
                     /locus_tag="ACHKFK_004772"
     CDS             3586..>3877
                     /locus_tag="ACHKFK_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFO9054917.1"
                     /translation="MAAYLLKKHGGSMRFIKLMYLSDRKAMELYGFPISGDHYFSMNK
                     GPVLSQTYSLVKDGSENEYSPWESWISDVADHKVEMWRTFSSVDELGELSPAE"
BASE COUNT         1007 a          874 c         1154 g          842 t
ORIGIN      
        1 ttgttcgcga aactattcgc acgttaggcc agcgtgtgcg ggaaggttac acgccgcgtg
       61 aggatggcgc tggcgcatcg tctcagtact cccgtagcgg taagcctgat ccgttcaaag
      121 tgccggaagg tgagggcgag cgttatcgtg atgaccttgc cagaatgatg aagtctctgc
      181 gcaccacaga tttaacggta aacatcgggc gtacgccgcc ggtattgcgt caccttggcg
      241 caccagattt gccgctggtt atctcccgcg acactgtgcg taaggccacc aatggagtga
      301 aacatgtggt gccgatggat gttatcgaga gactaccgga acttatgcac gatccggatg
      361 caatttaccg ttccgcgaca gaaagaaatg cggttgtgat gctgcttgat gccatggata
      421 aaaatggtga tcctgtagtg tcagcggtac acatgaaggc tgtccggtcg cgcctggaaa
      481 tcaacaaggt agcttcggtt tacggtacgg aaggaggaat gaataaggca aatagtctgg
      541 ataaagccgg attaacgctt taccggaaga aaaaattaag ccgcgataac cttctgcaca
      601 gagggcttca attgcccaaa ggggaacatt cttatcgcgg ctctgtagat aaaatactct
      661 atcctgaaga tattcgcaag gggccgtatt actcccgtac cagcagtctg acatcggaag
      721 agacaattgc atcccgtttt gttcgccaga tgcaggataa attccaggtg ctgaaggcgg
      781 tgcaggagaa tatccgtaaa actggcggaa aaatagatga cagcaataac gcctatatgg
      841 ctgaagaact cttccacggg aaggcggaaa acgacctgaa cgtaatgaag gagcgctacg
      901 ttcagccgct ggccaaattg ctggcggact acaaaattgc gcaggccgat ctggatgagt
      961 acctctacgc ccgccacgcg ccggaacgta acgcgcatat tgcgaaaatc aacccgaaaa
     1021 tgccggacgg cggttcgggg atgaccaacg cggaagcggc ggaaatcatg cagcgtgtgc
     1081 gtaacagcgg caaacaggca cagtatgacc gtctggcagg gattattgac gatatgctgg
     1141 cccgtcgccg tgagattatc cgtgaggcag gactggaaga gagcggcgtg gtggatgcct
     1201 ggcagaaagc ctaccgttac tacgtccccc tgaaagggca ggatgttgac ggtgtggtgt
     1261 cactgccacg tacaggcaag ggattcacca tcggcgggcg tgaaagtaag caggccatgg
     1321 ggcgtgcatc ccgcgcacag tctccttcca ctcaggcgat acaggatttg agtgaatcgc
     1381 tgatccgcaa tcgcaaaaat gaagtgggta acgccttcct gaaactggta caggataatc
     1441 ccgacaagga ttactggcag gtattcactg atgacagacc ggatacaatg cgtgtgattg
     1501 cagagcgcaa ggaccaggaa actggtgaaa ccattcgcga agttgtcgag cgccctgtgc
     1561 cgatggcaat gatggcggat cgctacttca ccaccaaaaa ggacggcaaa acttactaca
     1621 ttaaacttca tgatccgcgc ctgatgcgtg cgatgaagaa catggggccg gaaaccagta
     1681 acgccgtaat ccgtacgctg gggaaagtta accgtttcct ggcaacggtg aacacgtcgt
     1741 ataacccgga atttctggtc agtaacttca tccgtgacgt gcagacggcg gtgatgaacc
     1801 tgaaggcgga gcagggaagg agcgacggta aactgaaagg gctggataac ttatccgccc
     1861 tggctgtggt gaaagacagc cggtctgcca tgtctgccgt atacgccagt ctgcgtggta
     1921 aaaccctcac gggcaacggt gcgcagtggc agaaggtgtg gaaagagttt gttgaggacg
     1981 gaggtaaaac cggctggttt aacatgggtg accttgaagg ccagcagaag gaaatggatc
     2041 gccttgtatc gctggcgaag ggaggatgga aaggccagag tatcggtgca tggaattcgt
     2101 tccttaacct tgtcgaggat gccaacgggg cggttgaaaa cgctctgcgt ctttctgcct
     2161 ataaacacgc ccgtgatgct ggtttgtcac gccagcaggc ggcgtctctt gccaaaaaca
     2221 tgacggtgaa ctttaaccgt cgtggtgagc agggagcgct gatgaactcg ctgtatatgt
     2281 ttgccaacgc cagcattcag gggaccgcaa acctggtgag aacgctcgga catcttaatg
     2341 gcgacgggcc gttactggag cgccttcgct ggaagaatct caatgtaccg cagaaaatcg
     2401 cgcttgcagc tgtgggagcg ggttatctgc ttggctcgct taaccgcagt gtggcgggtg
     2461 aggatgatga cggggttaac tggtacgaca aggtgccgta tcatgtgaaa gagcgtaacc
     2521 tcgtcattat gaaatcgatg ttcggggggc agggccggag agtactggag tattcctctg
     2581 ccttacgggt acaacgtttt cttcctgctc gggcatactg ctgaaggtgt ggcagcgggc
     2641 gacctgactg cttcacgtgc tgccggtaat gttgtcggtg gtatcctggg ggcattcagt
     2701 cctgtgggca gtgaaacgtc ggaaacactg tccggggcat tgctgaaaaa tgcagcgccg
     2761 accattctgc gtccgtttgc gaaccttgcc atgaatgaaa acttcatggg ggcgcagatt
     2821 taccaggaga acatgccatt tggcacactg aaaccggaca gccagctggg cagacgttca
     2881 acgcctgaga cgtacaaggc gtttgcatcc tggctgaatg cgttctcagg tggcagccag
     2941 tatcgtcccg gcgcggtgga tatcacaccg gaatcgctga aattctggat tgactatatc
     3001 tccggaggga cagggcgctt catttccaaa accacagatg cggcggtgaa atcgctgaac
     3061 ggtattgata taccggagca gcaggtgccc ttcctgggga aaatttcggg tgaggtgatg
     3121 ccgtatgcag accagcagaa gatgtacgac cggatgacag aggttgcgca gtatcacgca
     3181 gagctgaaga gtctgaccgg tgcagaaaga acggcgttca ttgacgagaa caacggaaaa
     3241 ttgttgatga acgggcttat gcaggatacc cggaagagac tgaaggattt gcgcaaacag
     3301 cgcgatgcca tttatgccga cagttctctc agtctggcgc agcaggcggc gatggtgaaa
     3361 tcggtagagc gggatatgaa ggttgccgtg gatcgcttta accgcgagta caacaaaaaa
     3421 gtgggagtgg attaacagaa atggccccgt acggaagtgc ggggctgatt aagaaataaa
     3481 cacacattaa cctgtaataa ccggagctat taacatatag tcagaaagag tatttcatgt
     3541 gagacagaga gccgatttat gtttaatgaa gaaaaagttg cgtaaatggc agcgtatttg
     3601 ctgaaaaaac atggcggatc tatgcgtttc attaagctga tgtatctctc tgaccgcaaa
     3661 gcaatggagt tgtatggttt tcccattagt ggtgatcatt atttctctat gaacaaagga
     3721 cccgttttat cgcagacata ttctttggtt aaggatggaa gtgaaaacga gtacagtccg
     3781 tgggaatcat ggatatctga tgtagcggat cataaggttg aaatgtggcg tacattttct
     3841 tctgttgatg agctggggga gttatcgccc gcagaaa
//
LOCUS       DAZGJT010000212         3710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZGJT010000212 DAZGJT010000000
VERSION     DAZGJT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.217"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..968)
                     /locus_tag="ACHKFK_004773"
     CDS             complement(<1..968)
                     /locus_tag="ACHKFK_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraI domain-containing protein"
                     /protein_id="HFO9054918.1"
                     /translation="MFKLRKFFTSGSAVEIPDDGRSVPGTPEGFYAPLTTDSLLMPKN
                     RAQCLKQIRDNNMMPKPLCEAMCCAPVYSLLLSTQNVPAAREGRWSHAGGFGDLTLLH
                     TSYAVRLARAHMFPPGASAEEQAAQGAAWHAVIFWSALFYHLPLLAHLEGKLFSGRGW
                     QPGISVPDEAYRFRFRQLPPQGTEAQQLAAVIAGGLLPEGAAAWLATVPGALQNLAGA
                     FWHQHPEMALIRDVLQEAARQAESPLLNGGIVATASHIASAEVFTGEVVVSGKTESTV
                     ASAVSGEEAAAEGNTVSGNAAESVQEQPEMRQQQAEKPTTPDSSEGS"
     gene            complement(961..2469)
                     /locus_tag="ACHKFK_004774"
     CDS             complement(961..2469)
                     /locus_tag="ACHKFK_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HFO9054919.1"
                     /translation="MPHKPTEEQTAIIHWQGRKLVVNAFAGTGKTSTLVSYAMACPES
                     RMLYLAYNRAIRDEAERRFPFNVECKTSHQLAFARFGRHYQKRLVPGLFVTDVARKLN
                     TRYWALARVAMTGLNQFICSADATPGRQHLPDKDEMKGVAPDEALRAVRTLWQAMSDP
                     DGTFPVTHDTYLKLFQLSGPDLSRRWDTILFDEAQDANPVTSALVLGQRCRIVLVGDR
                     YQQIYRFRGADDALSHPSLAGADRLWLTQSFRFGPAVAAMANLLLKRAGESREVSGCG
                     GDDEVLTMQEMRERMKRGKFGGHRAVLNRTVAGVIGTALMAAMAGRKVYWVGGIEGYR
                     TEALEDLYWFQMDMPARMHSDRLRRDYRNFDEYRHIAKVTKDVEMSQSIRLLELCFPL
                     PKKLELLREHTVTNEREADITVSTAHRSKGLEWERVVLGDDFQDIADPLMSEQERRDE
                     TNLLYVAVTRARRTLVLNELMQQLAEEKSRTKEKTTSPHQRGTEKAVEGKDV"
     gene            3009..3239
                     /locus_tag="ACHKFK_004775"
     CDS             3009..3239
                     /locus_tag="ACHKFK_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFO9054920.1"
                     /translation="MPSAQATPVPYKYCIQATIEKPGGTPVSWIRFQNTPMTKQECEK
                     LFSTEKEAGKSFTVKVGVTQFSCKENPPAPGE"
BASE COUNT          860 a         1142 c          836 g          872 t
ORIGIN      
        1 gaaccttcac tgctgtctgg tgtcgtcggt ttctcagcct gttgttgccg catctctggt
       61 tgttcctgaa cagactctgc agcattgccg gacacggtat tcccttcagc cgcagcctct
      121 tccccggaca cagccgatgc cacggtgctc tccgttttac cggatacaac cacctccccc
      181 gtaaagacct ctgctgaagc aatatgactg gctgtggcaa caattccacc gtttaacaac
      241 ggactttcag cctggcgggc cgcctcctgc agaacatcac ggatgagtgc catttccgga
      301 tgctgatgcc agaacgcacc ggccagattc tgaagcgctc ccggcaccgt cgcaagccag
      361 gctgcagccc cctcaggtaa cagaccaccg gcaatcactg ccgccagttg ctgagcttcc
      421 gtaccctgtg gcgggagctg cctgaaccgg aaacgatacg cctcatcagg aacggatatt
      481 cccggctgcc atccccggcc gctgaacaat ttgccttcca gatgcgcaag cagaggcagg
      541 tgataaaaca gggctgacca gaaaatcact gcatgccagg ccgccccctg tgcggcctgc
      601 tcttccgcag aggcgcccgg cggaaacata tgtgcacggg ccagacggac agcgtaactg
      661 gtatgcaaca acgtcaggtc cccgaatccc cctgcatgtg accagcgccc ctcccgggcc
      721 gccgggacat tctgcgtgga gagaagcagg ctgtatacgg gagcacaaca catcgcctca
      781 cacaatggct ttggcatcat attgttatcc cgtatctgct tcagacactg cgccctgttc
      841 ttcggcatca gcagtgaatc tgtcgtcagc ggcgcataaa agccttccgg tgttcccggc
      901 actgaccgcc cgtcatcggg tatctccact gctgaaccgg aggtaaaaaa cttccgtaac
      961 ttaaacatcc tttccctcca ccgccttctc tgtgccacgc tgatgcgggg acgtcgtctt
     1021 ctccttcgtc ctgctctttt cctccgccag ttgctgcatg agttcattga gtaccagcgt
     1081 cctgcgtgcc cgggtcaccg ccacatacaa caggttagtt tcatcccggc gctcctgttc
     1141 tgacatcaac ggatcagcga tatcctgaaa atcatccccc agtacaaccc gctcccattc
     1201 cagcccctta ctgcggtgcg ccgttgaaac cgtgatatca gcctcccgct cgttcgtgac
     1261 tgtgtgctcc ctgagtagct ccagtttttt gggtaacgga aaacacagct ccagtagtcg
     1321 tatggactga ctcatttcca catccttcgt caccttcgca atatgacggt attcatcaaa
     1381 attccggtaa tccctgcgca accggtcaga gtgcatccgg gccggcatat ccatctggaa
     1441 ccagtaaaga tcttcaagcg cctccgttcg gtatccctca atgcccccga cccagtaaac
     1501 cttcctgccc gccattgccg ccatcagcgc cgtgccaatc actccggcga cggtccggtt
     1561 cagtaccgcc cggtgtcccc caaattttcc acgcttcatg cgctcacgca tttcctgcat
     1621 ggtaagcacc tcatcatcac caccgcagcc gctcacctcc cgactttccc ctgcgcgttt
     1681 cagcagcaga ttagccatcg cggccactgc ggggccaaaa cgaaaactct gtgtcagcca
     1741 gagacggtcg gcaccggcca gagacggatg agacagggca tcatccgcgc cacgaaaacg
     1801 gtagatctgc tgataccggt caccgaccag cacaatcctg caccgctgtc ccagcacaag
     1861 tgcggaagtc accggattcg catcctgcgc ttcatcaaac agaatggtgt cccatctgcg
     1921 cgagagatca ggtccggaga gctgaaacaa tttgagatag gtgtcatggg tgaccggaaa
     1981 cgttccatcc ggatcgctca tggcctgcca gagcgtcctc acagcacgaa gtgcctcatc
     2041 cggagcaacg cctttcattt cgtccttgtc cggcagatgc tgcctgccgg gggttgcatc
     2101 cgctgagcag ataaactgat tcagccctgt catggccacc cgggccagtg cccagtatcg
     2161 ggtattcagt tttcttgcca catcggtgac gaacaagccc ggaacaagcc gcttctggta
     2221 atgccttccg aagcgggcaa acgccagctg atgcgacgtt ttgcattcaa cgttaaaagg
     2281 aaacctgcgc tcagcttcat cacgaatcgc acgattatag gcgagataga gcatcctgct
     2341 ctccgggcag gccatggcat agctgaccag tgtgctagtt ttccctgtac cggcaaaagc
     2401 attaacaaca agcttcctcc cctgccagtg aataattgcc gtctgttctt ctgtcggttt
     2461 atggggcatg gctttcctcc gtaaagacga aatttccggt cactctgcct tcagccaggc
     2521 gaccgtgacc agcattaatt taccctggtg accagaaaat tttaccgccc gtaaattttt
     2581 agcgacctga aaagaattaa ttatcgccac ccgggtatcc gctgttcatt atcccctcag
     2641 acacagccca caacgggcct gctgtatcac cgttcttcgt aacggtcatg ctctttaaca
     2701 atccgggagc ggccacgccc tgaagccggg ctgaggagga aaacagatat gctacgtaga
     2761 gagtgacgtg ccgcgcctgc agccacaggt aacactcccc caacaccgga caccgcaaat
     2821 aatggctaca caacccgaaa acgcactgag cgccagaaca gtacgtttcc ggttgtgccg
     2881 gagcctgcac cctgcagagt cctgaacaac agccagtaaa accaggcact ttcggcgata
     2941 tcattttccc catggtgatg tcgccttttt tcagcacaga atgataatgc acagcaggag
     3001 tacagataat gccatcagca caggccaccc cggtcccgta taaatactgt attcaggcca
     3061 ccatcgaaaa acccggcggt acaccggttt catggattcg cttccagaat acgcccatga
     3121 cgaaacagga gtgtgaaaaa ctcttctcca ctgaaaaaga agccggtaaa tcattcaccg
     3181 ttaaagtcgg ggttactcaa ttttcatgta aggaaaatcc acctgcacct ggagagtgat
     3241 ttttattatg cctcatccca tcacatatac aaattttctt ccggtggttc tgtttttcgt
     3301 tatcgttatt tttattatca cttttataat agccatcacc acgaccatcc ccgcagatat
     3361 gactgtaaga ggcttttaca atagatacag aaaggatttt ctgtctcttt tttcggcata
     3421 tttcctttca tcaatattct gagcattcct ggtgacaggc accagtattt cagacagcac
     3481 gccggttttc ggtagtacac cggacaatgc gattacaaac tcgctgattt cactggcgtt
     3541 ctgtaattcg ttattaactc acgcatggat ttgtgatctc tgcgacatac acagaaacaa
     3601 aaacaccttt atttatataa ccctggcttt tcttcatctt attttaatca attcatgcac
     3661 ccattttatc tacgccactt tttattaatt cagttaactc cgtcttaaca
//
LOCUS       DAZGJT010000213         3513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZGJT010000213 DAZGJT010000000
VERSION     DAZGJT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.218"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1111
                     /locus_tag="ACHKFK_004776"
     CDS             <1..1111
                     /locus_tag="ACHKFK_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HFO9054921.1"
                     /translation="HYSNYLKNLEDLATSSGINSEAFITKSKAFNTVIRRALGCKLRT
                     YISKNYSDLSFVCDVPLDWIRFNNIPIMFSHEISRINATPGNVLLQSASAFPRVLVKA
                     SELRKVLVIRSFEPDDHLKFILENAIEIFKKQMPDLDCEIIDVRSKAEFIDALNQYQG
                     HVLVMDCHGNHDGNGSHGWLIIGEDKVDTWSLRKIARIPPIVILSACLTSALSGSHAS
                     VANGFVISGALSVIGTLLPVNAIDSAVFVSRLIYRFYEFPSTLSTNFTHVNVRLFLSV
                     FLRMSYASDLIRGFLSEDLIPNNSWRKDAVDINMYINMLHHDWYDYVINKLTMLTGLT
                     KKDVLDFIDRKLFITETMCYSQIGFPDAITISLKD"
     gene            complement(1413..1652)
                     /locus_tag="ACHKFK_004777"
                     /pseudo
     CDS             complement(1413..1652)
                     /locus_tag="ACHKFK_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(2033..2395)
                     /locus_tag="ACHKFK_004778"
     CDS             complement(2033..2395)
                     /locus_tag="ACHKFK_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054922.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRTFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVIPKAPEAGA"
     gene            complement(2386..2922)
                     /gene="yfdR"
                     /locus_tag="ACHKFK_004779"
     CDS             complement(2386..2922)
                     /gene="yfdR"
                     /locus_tag="ACHKFK_004779"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO9054923.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKQMEEKIDSVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPLSPGHA
                     YGMFMERFNELSELRKCA"
     gene            complement(3050..>3513)
                     /locus_tag="ACHKFK_004780"
     CDS             complement(3050..>3513)
                     /locus_tag="ACHKFK_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2303 family protein"
                     /protein_id="HFO9054924.1"
                     /translation="LLSVNGERNSQKSLAEWIEDWADYLVGFDANGDTIQATKAAAAV
                     RKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCIPFEGLKERPFKLR
                     LSIITGDRPVLVLRIIQLEAVQEEMANEFRDLLVEKFKDSKVETFIGTFTA"
BASE COUNT          944 a          783 c          734 g         1052 t
ORIGIN      
        1 tcattactcc aattatttga aaaatcttga agatcttgca acttcttctg ggataaatag
       61 tgaagcattc atcactaaat ctaaagcatt caatactgtt attcgaagag cccttggttg
      121 caagttgaga acatatataa gtaaaaacta tagcgatttg tcctttgttt gtgatgtacc
      181 attggattgg attaggttta ataacatacc tataatgttc agtcatgaga tatcaagaat
      241 aaatgctacg cctggtaacg ttcttctaca gagcgcctct gcttttccgc gcgttttagt
      301 aaaagcatct gagttgagaa aggttttagt tatacgttct tttgagcctg atgatcactt
      361 aaagttcata ttagagaacg caattgagat atttaaaaaa cagatgccag atcttgattg
      421 cgagattatt gatgttagat cgaaagctga gtttattgat gctctaaatc aatatcaagg
      481 tcatgttctt gttatggatt gtcatggaaa ccatgatggc aatgggagtc atggatggtt
      541 aataataggt gaggataaag tggacacttg gagtttgaga aaaatagcaa gaattcctcc
      601 tatcgtaatt ctgagtgcat gtttaacatc tgccctaagc ggttctcatg cctctgttgc
      661 taatggcttc gttataagtg gtgcattaag tgtcatcgga acattactgc cagtaaatgc
      721 gatagactct gctgtgtttg taagtcggtt gatctaccgg ttttacgagt ttccttcgac
      781 gctatctaca aattttacac atgttaatgt aaggcttttt ctttctgttt ttcttaggat
      841 gtcctatgca agcgatttaa taagaggatt tttatctgaa gatttgatac cgaataattc
      901 atggaggaaa gatgcagttg atattaacat gtatataaac atgcttcacc atgattggta
      961 tgattatgta attaacaaat tgacgatgtt aacggggctc acgaaaaaag atgtacttga
     1021 ttttatcgat agaaaattat tcataaccga aactatgtgc tatagccaaa ttggatttcc
     1081 agatgcaatc acaattagtc ttaaagattg acaactgtat gtaataaata tggcatactt
     1141 ccggcacaga gcgaatataa cagatcaggt ttagctctgt gccataggtg taccaactct
     1201 catctgagct aatgctcgtt actcaataac tcccgcaaat ttgtaaatct tgcgtgatgc
     1261 ccatttattt gggcatgatt taatatcagg atctggaaag tctggcctgt atttctggcc
     1321 agttctcctg tttacgctgt tccagcgaag aacggtcgat actgaaacgc cacagaagtc
     1381 ggcgacttgt ttagttgtca ttaagttgtt cattacttca cctcctgcgg tggctccggt
     1441 agcggcatcc agtgggttac tttcgatgcc ggttcttccc catcgtcagt aactgcccac
     1501 catttgtttc tcgaccaatc gtaataccct tcgaaggtat cgcactcagt ccagccgtaa
     1561 gacttacccc aacaccaaac atactgttta tcgtccggca ttcgctcact acagcttatc
     1621 caaccatccg gagttaccgg agagttgccc gacagctcgt tcaacttgta agtctggctt
     1681 acaggttcgg caccatgaag catggcggcg cggcaggcgt tccagccttc atcaaagccg
     1741 actatgccat tatttaaaga cggacgaaca tctggcacca tcggcactgg cttggctata
     1801 tatagcggct gaacatacca gccctttgat aaccaactgt cagcaatgtt tttactccgt
     1861 gttattgccg gaatacctaa gccattgtct gaatgcagcc atgccaccgg ttctgcttcc
     1921 agcgatacca aagcaatttc ataagcacgg cgctcaatat tgtcttgcac atccagactg
     1981 cctatgcgct ctttgatttc tttaatcagt tctttgtcgg taaatgtggt cattatgccc
     2041 ctgcctccgg tgcctttggt attactgccc agtgagtgat attgacgttt tcaagatccc
     2101 cgacctgaaa tgtccactgc cattctccgg tttccttttg tccccaggtg taccagagag
     2161 aacgccagcc aatcagccag ccttctccgt tagcatcaaa taacagaaca ctttcatttg
     2221 caggtggcaa ttcagctgac actggtatta ttttgttttc cagtgccgca catttagctt
     2281 caagcgcgtc gaatgtacgt actaggtact cagcatttgt ttcgttcact ttcagatctc
     2341 gcggtacaca tttcccgcga agaaaccctt ccatttcgaa aacattcatg cgcatttgcg
     2401 taactccgat aactcgttaa aacgttccat aaacatcccg taggcatgac ctggcgacag
     2461 tggaataact ttgaacatct ctgttgccgg gataccttcc aatacaggcc agaaagagcc
     2521 atcatcaagt ccgagatcgc ggcgttcggt tgccagcata atgagatcgg catatttcac
     2581 gggcgtactc ataactgggg gtaatccgta tttctcacgg attacggagt ctattttttc
     2641 ttccatttgt ttatagtcag gaagaaggcg tttcagtggt gcgggaatgt cctggcaata
     2701 cgcttctgtt gcatcatgca ttaacgcttc aaaagcaaat tcctgcggca ccagctggct
     2761 gcaaagaacc gcatgttggg cgacgctgta gaagtgcgaa agatgaccgg caaagcgaca
     2821 gatatttgaa agggaaaccg cgatatcgtt aatatcgatg tcgtctttat ttatcctgtc
     2881 ataataaaaa tgcttcccgg aaaaagtttt aataaatgac attttgttct ccacgtatat
     2941 gcgctgcacc gcgctgaatt ctggtaaaaa gaatccctca ccatccggcg attattgagt
     3001 aaattacgtt tccataaatg cccccgcagg ggcatttgca gtaatgaaat caggcggtga
     3061 aagtaccaat aaaggtttct actttgctgt ccttgaattt ctcaacaagc agatcacgaa
     3121 attcgttagc catttcttcc tgcaccgcct ccagctgtat aatgcgcaga accagtaccg
     3181 gacgatcgcc agtgataatg ctgaggcgta atttaaacgg acgttctttc agaccttcaa
     3241 acggaatgca tttaaattca aatgccactg gcataatgtc tttggtcttc gcttcgacag
     3301 actccatcag ggagcgtttg ccgctgaagt cattatcttc aaaatcagcg gtctggtttg
     3361 cttcaatcgt gattttacgg accgccgcag ccgcttttgt tgcctgaatg gtgtcaccat
     3421 tagcatcaaa gcccacaagg tagtcggccc agtcttcaat ccattctgcc agtgatttct
     3481 gggagttacg ctcgccatta acagacaaca gag
//
LOCUS       DAZGJT010000214         3463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZGJT010000214 DAZGJT010000000
VERSION     DAZGJT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.219"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            347..1663
                     /gene="shiA"
                     /locus_tag="ACHKFK_004781"
     CDS             347..1663
                     /gene="shiA"
                     /locus_tag="ACHKFK_004781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO9054925.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRS"
     gene            1765..3219
                     /gene="amn"
                     /locus_tag="ACHKFK_004782"
     CDS             1765..3219
                     /gene="amn"
                     /locus_tag="ACHKFK_004782"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO9054926.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGRGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          820 a          827 c          818 g          998 t
ORIGIN      
        1 gcaatacgtc tcaacaatga tactaaaaac ataactaacc tcgcctccct actggttata
       61 atgcaatgca gtctatcaga atcatcaggg tgcaattttg tgcatatgtg gacttttatg
      121 tttcatatct cgaacctgtg ggccctctgc ttaatcctta aacaacacca gcaactcctg
      181 cgctttcatc ttccatcgaa tttttcatgt tgccgctaat cagccataaa atcatttgca
      241 gatgcgctct gtcgaggtag tctcataagg ttcgtttata gatcgacggc aatgtgagtt
      301 accttttcca tactaattat aaaaagacag tacaaacagg atcattatgg actccacgct
      361 catctccact cgtcccgatg aagggacgct ttcgttaagt cgcgcccgac gagctgcgtt
      421 aggcagtttc gctggtgccg tcgtcgactg gtatgatttt ttactctatg gcattaccgc
      481 cgcactggtg tttaatcgcg agtttttccc gcaagtaagc ccggcgatgg gaacgctcgc
      541 cgcatttgcc acctttggtg tcggcttcct tttccgaccg ctcggcggtg tcattttcgg
      601 tcactttggt gaccggctgg gacgtaagcg catgttaatg ctgaccgtct ggatgatggg
      661 catcgcgaca gccttgattg gtattcttcc ttcattctcg accattgggt ggtgggcacc
      721 tattttgctg gtgacactgc gtgccattca gggatttgcc gtcggcggcg aatggggagg
      781 cgcagcgttg ctttccgttg aaagtgcgcc gaaaaataaa aaagcctttt acagtagcgg
      841 tgtacaagtt ggctacggag taggtttact gctttcaacc ggactggttt cattgatcag
      901 tatgatgacg actgacgaac agtttttaag ctggggctgg cgcattcctt tcctgtttag
      961 catcgtactg gtactgggag cattgtgggt gcgcaatggc atggaggagt ccgcggaatt
     1021 tgaacaacag caacataatc aagcggccgc gaaaaaacgc atcccggtta tcgaagctct
     1081 gttacgacat cccggtgctt tcctgaagat tattgcacta cgactgtgcg agttgctgac
     1141 gatgtacatt gttactgcct ttgcacttaa ttattcaacc cagaatatgg ggttaccgcg
     1201 cgaacttttc cttaatattg gtttgctggt aggtggatta agctgcctga caattccctg
     1261 ttttgcctgg cttgccgatc gttttggtcg gcgcagggtt tatatcacag gcgcgttgat
     1321 cggaacgttg agcgcatttc ctttctttat ggcgcttgaa gcacaatcta ttttctggat
     1381 agttttcttc tccataatgc tggcaaacat tgcgcatgac atggtggtgt gtgtgcaaca
     1441 accgatgttt accgaaatgt ttggtgccag ttatcgctac agtggtgctg gagtcggtta
     1501 tcaggttgcc agtgtggttg gcggtggatt tacacctttt attgccgctg cactcatcac
     1561 ttactttgcc gggaactggc atagcgtcgc catttatttg ctggctggat gcctgatttc
     1621 tgcaatgacc gctttattga tgaaagacaa tcaacgctcg tgatagcctg gcgaagatca
     1681 tccgatcttc gccttatact tttgtttcac attcctgtga catactatcg gatgtgcggt
     1741 aattgtatgg aacaggagac acacatgaat aataagggct ccggtctgac cccagctcag
     1801 gcactggata aactcgacgc gctgtatgag caatctgtag ttgctttacg caacgccatt
     1861 ggcaaatata ttacaagtgg cgaattacct gatgaaaacg cccgcaaaca aggtcttttt
     1921 gtctatccat cactgaccgt aacctgggac ggtagcacaa ctaatccccc caaaacgcgc
     1981 gcatttggtc gttttactca cgcaggcagc tacaccacca cgattactcg ccctactctc
     2041 tttcgttcgt atctcaatga acaacttacg ttgctgtatc aggattatgg tgcgcatatc
     2101 tctgtgcaac cctcgcagca tgaaatccct tatccttatg tcattgatgg ctctgaattg
     2161 acacttgatc gctcaatgag cgctggttta actcgctact tcccaacaac agaactggcg
     2221 caaatcggcg atgaaactgc agacggcatt tatcatccaa cggaattctc cccgttatcg
     2281 cattttgatg ctcgccgggt tgatttttcc ctcgcacggt tgcgccatta taccggtacg
     2341 ccagtagaac attttcaacc gttcgtcttg tttaccaact atacacgtta tgtggatgaa
     2401 ttcgttcgtt ggggatgcag ccagatcctc gatcctgata gtccctacat tgccctttct
     2461 tgtgctggcg ggaactggat cactgccgaa accgaagcgc cagaagaagc catttccgac
     2521 cttgcatgga aaaaacacca gatgccagca tggcatttaa ttaccgctga tggtcggggt
     2581 attactctgg tgaatattgg cgtgggaccg tccaatgcta aaaccatctg cgatcatctg
     2641 gcagtgctac gcccggatgt ctggttgatg attggtcact gtggcggatt acgtgaaagc
     2701 caggccattg gagattatgt ccttgcacac gcttatttac gcgatgacca cgttcttgat
     2761 gcggttctgc cgcccgatat tcctattccg agcattgctg aagtgcaacg tgcgctttat
     2821 gacgctacca agttagtcag cggcaggcct ggtgaggaag tcaaacagcg gctgcgtaca
     2881 ggtactgtgg taaccacaga tgacaggaac tgggagttac gttactcagc ttctgcactt
     2941 cgttttaact taagccgggc cgtagcaatt gatatggaaa gtgcaaccat tgccgcgcaa
     3001 ggatatcgtt tccgcgtgcc atacgggaca ctactgtgtg tttcagataa accgttgcat
     3061 ggcgagatta aacttcccgg tcaggctaac cgtttttatg aaggtgctat ttccgaacac
     3121 ctgcaaattg gcattcgggc gatcgatttg ctgcgcgcag aaggcgaccg actgcattcg
     3181 cgtaaattac gaacctttaa tgagccgcca ttccgataat aagttctgaa ccttctcatc
     3241 agaaatagat gtgaaggatt aagtaagaca tctggcaaat agcctgcaat caacgcgggc
     3301 tgtttgcttc cttgggcaga tgcgagtttc attatcgtct taatgatttc cgcatattaa
     3361 aagcatgtat gctttcaaaa tataattata aaaaatcccg ccaacaacat tagtttttat
     3421 aaaacaaaat ataagattat ggctttagaa tatttttatt tct
//
LOCUS       DAZGJT010000215         3412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZGJT010000215 DAZGJT010000000
VERSION     DAZGJT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.220"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..808
                     /gene="ydhY"
                     /locus_tag="ACHKFK_004783"
     CDS             182..808
                     /gene="ydhY"
                     /locus_tag="ACHKFK_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO9054927.1"
                     /translation="MNPVDRPLLNIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            829..2931
                     /gene="ydhV"
                     /locus_tag="ACHKFK_004784"
     CDS             829..2931
                     /gene="ydhV"
                     /locus_tag="ACHKFK_004784"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO9054928.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEDMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2944..3366
                     /locus_tag="ACHKFK_004785"
                     /pseudo
     CDS             2944..3366
                     /locus_tag="ACHKFK_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
BASE COUNT          909 a          749 c          893 g          861 t
ORIGIN      
        1 cacattattt tttctcctaa ccataaagga ttagtttgtc agcgttaaat cttttctcat
       61 acactatcgg gtttgggaaa atatctggtg ttgaggacag agtcagcagc gtattagttg
      121 agtgcctaat atcaggctgc tgaattgtca gcactgtgtt tgcgaagagg atgcgaaata
      181 aatgaacccg gttgatcgtc cactattaaa tattggctta acgcgattag aattcttgcg
      241 tatatcagga aaaggccttg ctggtttaac aattgcccct gctttactgt cacttttggg
      301 ttgcaaacag gaagatattg atagcggcac agtagggttg ataaatacac ccaaaggggt
      361 gctggtgacc caacgtgcgc gatgcacggg ttgccaccgc tgtgaaatct cttgtaccaa
      421 cttcaacgat ggctcagtag ggacattctt ctcccgtatc aaaatccatc gcaattattt
      481 ctttggcgac aacggggttg gctctggcgg tggcctgtat ggcgatctca actataccgc
      541 ggatacctgc cgtcaatgca aagagccgca atgcatgaac gtctgcccga ttggtgcgat
      601 tacctggcag cagaaagaag gctgtattac cgtcgatcat aaacgttgta ttggctgtag
      661 cgcctgtacg acggcatgcc cgtggatgat ggccaccgta aataccgaaa gtaaaaaatc
      721 ctcgaaatgt gtgttatgcg gtgaatgcgc aaacgcctgc ccaacagggg cgttaaaaat
      781 tatcgagtgg aaagatatta ctgtgtgaat cttgcaaagg aaaaaatcat ggctaacggt
      841 tggacaggta atatattaag agtcaatctc acgacaggaa atattaccct cgaagattcc
      901 agtaagttta aaagttttgt cggtggcatg ggcttcggct acaaaattat gtatgacgaa
      961 gtaccgccag gcacgaaacc tttcgatgaa gcgaataaat tagtttttgc taccggccca
     1021 ttaactggat ctggtgcccc ctgtagttct cgcgtaaata tcacttcact ttctactttt
     1081 accaaaggaa atttagtcgt cgatgcccat atggggggct tttttgcagc gcaaatgaaa
     1141 ttcgctggat acgacgtcat tattatcgaa gggaaagcga aatcaccggt atggctgaag
     1201 attaaagatg acaaagttag ccttgaaaaa gccgatttct tatggggaaa agggacgcgc
     1261 gcaacgacgg aagaaatttg tcgcttgacc agtccggaaa cctgtgtggc ggctattggt
     1321 caggctgggg aaaaccttgt tcctctctct ggcatgttga atagccgtaa ccacagcggc
     1381 ggtgcgggaa ctggcgcaat aatgggttcg aaaaacctga aagcgattgc ggttgaaggg
     1441 acgaaagggg tcaacattgc cgatcgtcag gagatgaagc gtctcaacga ttacatgatg
     1501 actgaactta tcggtgcgaa taacaaccat gtcgtgccaa gtacgccgca atcgtgggca
     1561 gagtattcag atcccaagtc acgttggaca gcacgtaaag ggctgttttg gggcgcggct
     1621 gaaggtggtc cgattgaaac gggggaaatt cctccaggca atcagaacac ggtcggcttt
     1681 cgtacctata aatccgtttt tgacttaggt ccggcggcag agaaatacac agtaaaaatg
     1741 agtggctgcc actcttgccc gatccgttgt atgacccaaa tgaatattcc tcgggtgaaa
     1801 gagtttggcg tgcccagcac aggtggtaac acttgtgtag caaactttgt ccataccacc
     1861 atcttcccga acgggccgaa agattttgaa gataaagatg atggtcgtgt gattggtaac
     1921 ctggttggtc tgaatctgtt cgatgactac ggcctatggt gtaactatgg tcagcttcat
     1981 cgcgacttta cctattgtta cagcaaaggg gtgttcaagc gtgttctgcc tgctgaagag
     2041 tatgcggaaa ttcgctggga tcaactggaa gcgggtgacg ttaacttcat taaagatttt
     2101 tactaccgtc tggcgcatcg cgtgggtgag ctgagtcacc tggctgatgg ttcatatgcc
     2161 atcgcagaac gctggaattt gggtgaagag tactggggct acgcgaaaaa taaactctgg
     2221 tcgccgtttg gctatccagt tcaccacgcc aatgaagcgt cagcgcaggt cggctccatt
     2281 gttaactgta tgttcaaccg tgactgcatg acgcataccc atatcaattt tattggttcc
     2341 ggcttgccat tgaaattgca gcgtgaagtg gcgaaagagc tttttggttc tgaagatgct
     2401 tacgatgaaa ccaaaaacta cacgccaatc aatgacgcaa aaattaaata tgctaagtgg
     2461 tcgctgttgc gggtctgttt gcataacgcc gtcaccctgt gcaactgggt ctggccaatg
     2521 accgtttcgc cgctgaaaag ccgtaattat cggggcgatc tggcgcttga agccaaattc
     2581 ttcaaagcga tcactggcga agatatgact caggaaaaat tagatttagc tgcagagcgt
     2641 atctttacgt tgcatcgcgc ctacacggta aaactgatgc aaaccaaaga tatgcgtaat
     2701 gaacacgatc ttatctgttc ctgggtattc gacaaggatc cgcagattcc ggtctttact
     2761 gaaggtacag acaaaatgga tcgtgacgat atgcatgctt cgctcacgat gttctacaaa
     2821 gaaatgggct gggacccaca gcttggttgt ccgacccgcg aaacattgca gcgtctgggg
     2881 ctggaagata ttgccgccga tctggcagca cacaatctac tgcctgcgta aggaatggga
     2941 gagatgaacc atcaggatga attgccgtta gcggaagtca gcgaaattga tgaggcgaag
     3001 cggcaatggt tgcaggggat gcgtcacccc gttgatacgg taaccgaacc agaaccggct
     3061 gaaatcctgg cagagttcat tcgccagcat tctgcggctg ggcagctggt ggcgcgcgcc
     3121 gtgtttctct cgccacccta ttcggttgca gaagaggaac tgtcagtttt gctggaaaac
     3181 attaagcaga acggagacta cgcggatatc gcttgtatga cgggaagcca ggatgattac
     3241 tattactcaa cgcaggccat gagtgaaaac tacgcagcga tgtctttgca agttgtggag
     3301 caggacattt gtcgcgccat tgcacacgcc gttcgttttg agtgccagac gtatcctcgt
     3361 ccttacatgt caacgacgga tgaaaagtga tccacttata tctccaccaa cg
//
LOCUS       DAZGJT010000216         3394 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZGJT010000216 DAZGJT010000000
VERSION     DAZGJT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.221"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..354)
                     /gene="bglB"
                     /locus_tag="ACHKFK_004786"
     CDS             complement(<1..354)
                     /gene="bglB"
                     /locus_tag="ACHKFK_004786"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO9054929.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYERLFDEMAQAGI"
     gene            complement(373..2247)
                     /gene="bglF"
                     /locus_tag="ACHKFK_004787"
     CDS             complement(373..2247)
                     /gene="bglF"
                     /locus_tag="ACHKFK_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO9054930.1"
                     /translation="MTELARTIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNSFTAMVIGGALVHPLILTAFENGQKVDALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPITFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLIPLIINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPGIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMFH
                     FITAKSQPAQVAQQEKTPEIITPEQGGICSPMTGDIVPLIHVADTTFASGLLGKGIAI
                     LPSVGEVRSPVTGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNVG
                     DKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQINAGEPLLSIIR
                     "
     gene            complement(2381..3217)
                     /gene="bglG"
                     /locus_tag="ACHKFK_004788"
     CDS             complement(2381..3217)
                     /gene="bglG"
                     /locus_tag="ACHKFK_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO9054931.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHMPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRIIE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT          929 a          877 c          726 g          862 t
ORIGIN      
        1 gatccccgcc tgcgccattt catcaaacag ccgctcgtaa aacgctaacc ccgcttcatt
       61 cggttcggct tcgtcgccct gaggaaaaat tcgcgcccag gcaatggaaa tacgcagaca
      121 ggtgaagccc atctcggcaa ataacgcgat atcttccggg taacggtgat aaaaatcgat
      181 ggcgacatct ttgatattct ctttccccag gatgcgcggt tccatttttc ccattacgcc
      241 atgaggctgt aaatctgagg tcgtgatccc tttgccatct tcctgccagg cgccttccac
      301 ctgattggca gctgttgcac caccccaaag aaatgtttct ggaaatgctt tcataattaa
      361 ctccttttat cgttagcgaa tgatggataa cagcggctca ccagcgttta tctgcgccgt
      421 gccgtggggt aatacgtccg taaaatcatc gctattactg attaataccg gcgtcgtcag
      481 atcaaatccg gcctcgcgaa tagcagggat atcaaaagaa atcagccgat cgcctgtatt
      541 gaccttgtca cccacattga cgtgagcgga aaagaatttg ccgtccagtt ttacggtatc
      601 gataccgaca tgaatcagga tctccacgcc atcatctgac tcaatgccaa tggcgtgtaa
      661 tgtggcgaac aacgaagcaa ttcgacccgt aactggagaa cgtacttcac caacagaggg
      721 cagaatggca atacctttac ccaacaggcc gctggcaaac gtggtatcag cgacgtgaat
      781 gagcggcaca atatctcccg tcatcggtga acagataccg ccctgctcag gtgtaataat
      841 ctctggtgtt ttctcttgct gggcaacttg cgctggctga ctcttagcgg tgatgaaatg
      901 aaacatcacc gtaccgacaa atgcgcaacc gatggcaatg acaccgccaa taacgctggc
      961 ccatacggtg aaatcaatcc ccgttgacgg gatggtttgc atgaaggtga aaatacctgg
     1021 caaaccaaag gagtaaactt tcgtttgcgc gtagccaatg atggtggccc ccaatgcccc
     1081 acttatgcag gcgataacga aggggtactt acgcggcagg ttgacgccat atactgccgg
     1141 ttcggtgata ccaaacagcc ccgtcaacgc cgccgatccc gctaccactt ttttctgcgc
     1201 atcgcgttcg cagaggaaga cgccgagcgc cgccccgact tgcgccataa tggcgggcat
     1261 taacagcggg atcatggtgt cgtagcccag cacggtgaag ttattgataa ttaaaggtat
     1321 caaaccccaa tgcagaccga acatcacaaa gatttgccag aagccgccca ttaccgcgcc
     1381 tgcaaatgca ggaaccgcct gataaagcca gagataaccg gcagcaatca gttcacttat
     1441 ccaggttgat agcggcccca ccagcagaaa tgtgatgggc gtgatgacca tcaaacatag
     1501 caagggtgtg aagaaatttt tgattgccga aggtaaccac gcattaagtc ggcgttccag
     1561 aatgctgcac aaccaggcag aaaaaataat gggaataacc gatgacgagt aattcaacaa
     1621 tgtgaccgga atacccagga aatccaggcc cagcgcatcc actttttgcc cgttctcgaa
     1681 ggcagtcaga attaatggat gcactaacgc tccgccaatc accatggcag taaatgaatt
     1741 gccgccgaag cgtttccctg cggtgtagcc caggattatc gggaagaacc aaaacaaggc
     1801 atcactggcg ctaaataaaa ttaaataagt accactttgt tcggtcgtcc actgaaaagt
     1861 gagtgccaga gccaacatac ctttcaggat cccggttgcc gccatcaaac cgatcagagg
     1921 cgtaaaaata cctgaaataa cataaacaaa gcggtttaac agattacctt tatcatcatt
     1981 ttccggtgcc tgttgcgctt tttcgccaag gcctgccacg ctgttaaccg ccaggaagac
     2041 atcggccaca tggttaccta tgacaacctg aaactggcca ccgctttcca ccaccataat
     2101 aataccgggg gtctttttca gtacctcagc ttgcgctttg ctttcatcct ttaacttaaa
     2161 acgtaatcgc gttgcacaat gcatcagact cacaatgtta tctgcgcccc cgactcctgc
     2221 gactattgtt ctggctaact ccgtcataac ttgccctcta ccgctttgcg gcaaaactcc
     2281 aaaaaaaaac ctgaaaaaaa cggcctgacg tgaatcaagc aatttttttc aggttttgcc
     2341 cgcttagtgc ggtaacaatc ctttattcag taataatatt tcagtgttct ttgcgcacgc
     2401 gctctatatt tatggctaaa aacataatct ctgcaggtga gattttacgt tgatactgca
     2461 aaccaataaa aatggcgatc cgctccgcac attgccatgc ttgcgggtaa ttttgcttta
     2521 ctgcttgttg taatgattca tcactatcgt taatcgatgc atgttcaata atacgccagg
     2581 ataaaaattt cagatgcgta accagtcgct gataactcaa gctttcttcc tggtaattaa
     2641 ggctgaactg aaattttatt aattgcagca tttctcgcat taattgcgtg acacctgcaa
     2701 catcctccat atttccgctc atttgggcac tgaccagatg catggcaata aagcccactt
     2761 catctttcgg taactgcacg cccaaccgtt tatcaataat ggttaacgct tcttccccta
     2821 gctggaactc tttcgggtaa agtcgctgga tatcccacag cagcgggttg ggcagtagca
     2881 cgttttgctg aaagcgttta atcgcaaact ggcaatggtc agttagcgag atataaatac
     2941 tgtcctgcaa ctttcccaga cgctcctgcg ccagagagat aatacgatca caggttgcca
     3001 tcacctcaag aggcatatga cttaagagtt cgcttaatcg cccgttcagt tcatgactgc
     3061 tcaaggcata ctctttttct attccacttg agttaattct ttcgcctggg cgtttttgaa
     3121 agccaattcc gcgccccatg acgacttttt cccgctgttg atcatcaata accaccacaa
     3181 cattattgtt gagaattttg gtgatttgca tgttcatagc aaggaccttt tttataaaca
     3241 aaaaaacccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
     3301 acaatccagt cattaattaa tggtttttat aacgaacatc caggttcgaa aattaattta
     3361 gttgcgtgca gagaaaatag caatgctcta ttta
//
LOCUS       DAZGJT010000217         3378 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZGJT010000217 DAZGJT010000000
VERSION     DAZGJT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.222"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            700..1386
                     /locus_tag="ACHKFK_004789"
                     /pseudo
     CDS             700..1386
                     /locus_tag="ACHKFK_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554952.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1719..2594
                     /locus_tag="ACHKFK_004790"
     CDS             1719..2594
                     /locus_tag="ACHKFK_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HFO9054932.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATAQHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQILALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            2602..3378
                     /locus_tag="ACHKFK_004791"
     CDS             2602..3378
                     /locus_tag="ACHKFK_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFO9054933.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTFS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
BASE COUNT          927 a          791 c          810 g          850 t
ORIGIN      
        1 ttcatcccca ctaactaaaa cgatccatac aataaaacta aatctaattg caggcaatca
       61 attccagtcg atatatcatc tgtatggaaa tcatcgctca tcactcatcg cagcctgaaa
      121 gcccagagca acggggcagg tctgcatata agtggttaca cggcagaatt ccagagggtt
      181 ctatcactaa attcagtcac tgacaaattt gcatgatgtt gaatagacga gattttgtgt
      241 cgaaatagtt gtttcagtaa ctgtcactca atcggtggtt tatgtgacta tattcgcaaa
      301 aactggtcgc agaaaagtcc tgttttcagc ttggaaaccc ggtaatcaga ataatggata
      361 ggggaacaaa aatctctgta tagaacgctc cattgaggga gagaaaacgt gtcattttga
      421 cccgttcggt attcagaaca cacaaaaccg atcgcacatc gctttcgtgc atagttatgc
      481 aacgccctga aaacgattct gacgcgtttt tctggtttgt cctggtgttt tcttgtcttt
      541 ttgcgttttt tgcgccagaa agcgattgag ggcgttttaa ggggtacgta caacgggagt
      601 tatggtaaat ggatcgggtt tgcgggaagg ttcgacagga tttgccgttg ggtgtagtgt
      661 aagcgactga aaaacaaacg cccctgaaat catgtccaga tccggcagaa ccagcctcat
      721 gcgcagttct ggctggtttt tgacgttgat cgcgaggggg cagcgattga ctggagcgac
      781 cggaacgctc ccgcgccgaa catcaccgta aaaaatcctg tgaacggtca tgctcacctg
      841 ctctacgcgc tcaatatcgc cgtgagaacc gcgccggaca gctcggttaa ggcgctgaaa
      901 tacgccgctg cgattgaacg ttcgctgtgt gagaaacttt gtgctgatgt gaactacagc
      961 gggctgattt gtaaaaatcc gttccacctg gaatggcagg tgatggagtg gcgagaggaa
     1021 gcctataccc tcgatgaact ggctgattat ctcgatttga gcgcctcagc gcgccgtagc
     1081 atcgataaac attacgggat ggggcgaaac tgccacctgt tcgaaatgac gcgcaaatgg
     1141 gcttacaggg cgattcgtca gggctggccc gaattctcac agtggcttga tgccgtgatt
     1201 cagcgtgtcg aaatgtacaa cgcatcgctt cccgttccgc tttcaccgcc tgaatgtcgg
     1261 gctattggca agagcattgc gaaatacacg cacaggaact tcacgccgga aactttcgca
     1321 cagtatgtgg ctgatacgca cacattaacg tacgttttcg ttccattggc cctcaccccc
     1381 cgataacgac ccaggtagcg cgccgtattc cccccgccgg ccgttttctt cgacatgtac
     1441 atcaggcgcg gaactcatcg gggcgcggga atgaaatccc attcagactc cggatagatt
     1501 caagcaagcc cacttatcgt caaaatcggt gttgcaaaaa cgggagtgac catagatccg
     1561 ttttccaagc ggaccccata atatcggctg gtaaacatca ctcgctagtg cattcttttt
     1621 gttggtaatg ataatcattt acattcattc tttgatgttt tatcataatt aatgttttgt
     1681 caataaatat atggaaacat attgtttcag atgggaatat gagaaagttc atccttattt
     1741 ctatgataac gttagctagt gttagtctgg gagcctgcag acagaatgtc accataaagc
     1801 aggatgcgcc agggcagaaa gctttcctga ctgacgggca gatttacgat ttgcacagtg
     1861 gcaaaattat ttctagcagc gagcttttgg cggatcttgc taccgctcaa catcttatta
     1921 ttggtgaaaa acacgataat gccgagcatc atcagattga gctttggctt attcaaaatt
     1981 tgctcatcca gcgaccacag gggagtgtgt tgcttgagat gttgacgtca gagcaacagc
     2041 cccgagttaa ccaagttaaa tgttggctga aagataatcc tgtggttcga gatagccgtg
     2101 ttcaggaact acttaactgg caaaaagggt ggtcctggga gatgtatggc gacatcgtca
     2161 tgcagctatt acgtgggcct tacccgctgc tcaatgccaa cattggccgg gagcagatat
     2221 tggcactgta caaaaaaaac gaatttccta aaggaaaaaa atccactgca cctgtggtac
     2281 aagaagctct gcgtgaaacc atcatatcta tgcatgaagg caaccttgaa agccagcaac
     2341 tgacgtcaat gctctctatt cagcagcagc gggatcgcta catggcccgc cagcttctga
     2401 gtgccccggt accgtcgttg ttgattgcag gtggttatca cgcgtcaaaa agcatgggcg
     2461 taccgttaca catggaagat cttgccaccg gcacccatcc agtggtgttg atgcttgcag
     2521 aaaaaggaat gaacatcaca gtagatcatg ctgactacgt gtggtttgta gcgccagata
     2581 caacaaaaag gtaaaccatg gatgatgaat attctccact tctcacagtc ggtaaaatgg
     2641 agcagctggt ttatctgttc tctactgctt catggactga ttttccttgc gtttatctgg
     2701 cgtttttccg aagttcagcc cgctatgtct cctgcaccgg cgatcatgct gcaatgggca
     2761 gaaattatag aggctccttc ttctccactt tcgcttccgg tgggaatcgc tcaacaggag
     2821 tccgcagtga cagaagaaaa gcaacaaact gaggacagac aacaacgtcc tgtcactgaa
     2881 gacagtgatg caaccattga aataactagg aagaaaaagt catccgatgg agaaaagaaa
     2941 aagacacgtc cacctcgtaa gataaaggct caaaccagcg acagcaaccc cacagccgtt
     3001 tccagtaatg ctgcacctca ggctctggtc gaatcgtccc gtattgctgc gccttttaac
     3061 agtgactcaa caaagcgaga taattctgag gcatcttggg aaagcagagt aaaaggccac
     3121 ctaaaccgtt ataaacgtta ccctggcgat gccagaaaac gtgcacgaac cggcacagcc
     3181 gtcgtgactt ttaccgtaaa cacggaaggc actattgtca gtagtttctt ggaaatctct
     3241 tcaggaacct tttctcttga ccgcgaagct attgctgttt tagaacgcgc tcaaccactt
     3301 cccaaaccac cgcctgaaat attggaggga ggattattta aagtaaaaat gccgattacc
     3361 tttaagttga aggagtaa
//
LOCUS       DAZGJT010000218         3303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZGJT010000218 DAZGJT010000000
VERSION     DAZGJT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.223"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..633
                     /locus_tag="ACHKFK_004792"
     CDS             <1..633
                     /locus_tag="ACHKFK_004792"
                     /inference="COORDINATES: protein motif:HMM:NF022136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HFO9054934.1"
                     /translation="AIIVTFCFVASSNASTDALAGVTLGQLLYNDSNKVDGTSVKSPK
                     KVSITEKIAANAVSQQLALNGNIPIKENESKKKEQVINNVKMSSKIVDTINNTIDRKQ
                     KIISITDSKTLNNSATYTNNNIAPLNWSVDKNQDLLNILNKWGKTAGWDVVWKSDYSY
                     RILTAASFNNIDFVTAVQRLFESMGEVNPRLYIKFYMGNRVVIISNKNDF"
     gene            646..2301
                     /locus_tag="ACHKFK_004793"
     CDS             646..2301
                     /locus_tag="ACHKFK_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secretin N-terminal domain-containing protein"
                     /protein_id="HFO9054935.1"
                     /translation="MRCIILLFLLFVSSCTTKQYQRSEHNINSNVNETNTALIKAKNL
                     EAKIDTSNIQISNGIYLASQSSKNIHGDPLPARFETNEGVTLVSGIPLKFDSILKLIS
                     DATGIPIVKSKAADLTSQNATSTISAENVSQSLNKAVGDGGDTNDSFFNSLVNYTVDA
                     HLMDVNYKGALSEFLDKLSANYDIVWKYRDGKLKFSSEDVRTFNIATLPSKITANNSV
                     GGAGSGSSGGGNSSSGGSSGSGGGNTGTQSLNIDLTLDFWQDLEKNLKIIIGNRGTFN
                     ISTSTSSVVVQTTPSNMERVADYIDNLNKELIRQVTVNVVVYSVNTTDSNNLALSLKG
                     ALSSGGRVLGSLSNEFAVANSPTLSGFINGDQDTNNSIIMNLLASAGKVSIVTSASVT
                     TMSGQPVPLQVGNDRTYVSEIGTTLGQTASTSTVSTSSISSGFAMNLLPRVMDNGNVL
                     LQYGINISSLAGSNNGFESVTVSGTTIQLPNVDQRTFVQSSLLRNGSTLVLAGYEQQR
                     DETGDQGVGRPDFKLLGGGKRSSNERNVIVICITPTVINIDKV"
     gene            2303..>3303
                     /locus_tag="ACHKFK_004794"
     CDS             2303..>3303
                     /locus_tag="ACHKFK_004794"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054936.1"
                     /translation="MFLRRKKKDTGDHFKSELIPGIVVIARKKYAVNLLWNTVHINDD
                     FRKSLNASAKSLDSKLYCKMDTLGGKQYAIADKKIGHKVGIETLLSKVNVSEGSVCAA
                     CTPDGDIWIAFAIDRDGIVYADKCSTNSDDIITNFNNILYSREWDRIYCPSEWGVANS
                     AEVDFAELLSSKGKKIKALGIGSYSAIIPLIALVFIILYVVKFYTTNSDIDVTPTVTE
                     MPADEPKVIEIEPPWKGNIKPQSLLTQCISSLHKRLFDISMIPGWDIDQNITCTNEGV
                     RFSLTKRDGLLLWFDATSKSFHDNPAISVSDSQATVTWSFDSLVYYENGSLDPKNLL"
BASE COUNT         1143 a          517 c          682 g          961 t
ORIGIN      
        1 gcgattatag ttactttttg ttttgtagct agcagtaatg cgtcaactga cgcattagcg
       61 ggagttacgc ttggtcagct attgtataac gattccaata aagtggatgg aacctctgta
      121 aaatcaccaa aaaaggtaag cattacagag aagatagcag caaatgctgt atcgcagcag
      181 ttagctttaa atggaaatat cccaataaaa gaaaatgaga gcaaaaaaaa agaacaagta
      241 ataaataatg taaaaatgag cagtaaaatc gtggatacga taaacaatac tatagacagg
      301 aagcaaaaaa ttattagcat aactgactct aaaacattaa ataatagcgc gacttatact
      361 aataataaca tagccccact aaattggagt gtagataaaa atcaagattt gcttaatata
      421 cttaataaat gggggaaaac agcaggatgg gacgttgtat ggaagtctga ctattcatac
      481 agaatactaa cagcagcatc atttaataat atagatttcg taacagccgt tcaacgattg
      541 tttgaatcaa tgggagaagt taatccaaga ttatacatta aattttatat gggaaacaga
      601 gttgtaatta tttccaataa aaatgatttt taaggcggat aaaccatgcg ttgcattatt
      661 ctgcttttcc tgttatttgt ctcatcctgt acaacaaaac agtatcaaag gagtgaacat
      721 aatattaata gtaatgttaa tgaaacaaat acagcgttga ttaaagcgaa aaatttagag
      781 gctaagattg acactagtaa tatacagatt agcaatggaa tatatcttgc cagccaaagt
      841 tcaaaaaaca tacatggaga tccattacca gccagatttg aaacaaatga gggggttacc
      901 ttagtatcag gaataccatt aaaatttgat agtattctaa aattaatcag tgatgctaca
      961 ggaattccaa ttgtaaaatc taaggctgcg gatcttacat cacaaaacgc aacgagtacc
     1021 atatcggcag aaaatgttag tcaaagtcta aacaaggcag ttggtgatgg gggggacact
     1081 aatgattcct tttttaacag tttagtaaat tatactgtag atgctcatct gatggatgtt
     1141 aactataagg gagccttatc agaatttctt gataaactaa gtgctaacta cgatatagtg
     1201 tggaaatatc gagatggcaa gttaaagttt tcatcagaag atgtacgaac atttaatata
     1261 gcaaccttac cgagtaaaat aactgctaat aattctgttg gaggcgcggg cagtgggagt
     1321 tccggaggcg gtaatagttc atccggtggt agttctggaa gtggtggagg taacaccgga
     1381 acgcaatctc ttaatataga tcttactcta gatttttggc aggatttaga aaaaaaccta
     1441 aaaataatta taggaaatag aggtacattt aatatatcga caagtacatc ttcggttgta
     1501 gttcaaacga caccatctaa tatggaaaga gttgctgatt atattgacaa ccttaataaa
     1561 gagttaatca gacaggtgac agttaatgta gttgtatatt cagtaaatac tacagacagt
     1621 aataatttag ccctgtcact aaaaggtgct ctgtcgagtg gtggaagagt tttgggttcg
     1681 ctatcaaatg aattcgcagt ggctaattct cccactctaa gtggctttat taatggtgac
     1741 caggacacga acaacagtat aattatgaat ttgttggcta gtgcaggcaa ggttagtatt
     1801 gtaacttcag cttcagtaac cacaatgtca ggccaaccag ttccactaca agttggtaat
     1861 gacagaacat atgtctctga aataggaaca acacttggcc aaacggcatc tacatcaaca
     1921 gtttctactt cgagcatttc atctggtttt gctatgaacc tgttaccgag agttatggat
     1981 aatggtaacg tattattaca atacggcatt aacatatctt cattggcagg tagcaataat
     2041 ggttttgagt ctgttactgt ttcaggaaca acaatacagc taccaaatgt agatcagcgt
     2101 acatttgtac aatccagcct tttacgaaac ggtagtaccc tagtgttggc tggttatgaa
     2161 caacagaggg atgaaacagg tgatcaaggt gtaggtcgtc ctgactttaa attacttggt
     2221 ggaggtaaaa gaagttcaaa tgaaagaaac gttattgtta tttgtattac tccaacagta
     2281 attaatatag ataaggttta acatgtttct gcgcagaaaa aaaaaggata ctggggatca
     2341 ttttaagtcg gaattgattc ctggcattgt ggtgattgcg agaaaaaaat atgctgtaaa
     2401 tttgctctgg aacactgttc atataaacga tgattttcga aagtcactta acgctagtgc
     2461 taaatcgctg gattcaaagc tttattgtaa aatggatact cttgggggga agcagtatgc
     2521 tatagcagat aaaaaaatag gccacaaagt cggtatagaa acactgttga gtaaagttaa
     2581 tgtgagcgaa ggttccgtct gtgcagcgtg tacacctgat ggtgacattt ggattgcctt
     2641 cgcaatagac cgagatggta ttgtatatgc tgataaatgt tctaccaaca gcgatgatat
     2701 aatcaccaac tttaataaca ttctttactc aagggaatgg gataggattt attgcccaag
     2761 cgagtggggg gtggccaata gtgctgaggt tgacttcgct gagcttcttt caagcaaagg
     2821 gaaaaaaata aaagcattag gcattggtag ttatagcgcc atcatccctc tgatagcact
     2881 cgtttttatc atactttatg tggttaagtt ttacaccact aacagtgata tagatgttac
     2941 tcctactgta acagaaatgc ctgctgatga accaaaagtg atagaaattg agcctccatg
     3001 gaaggggaat attaagcctc agtctctttt aacgcagtgt attagttcat tacataaaag
     3061 gctgttcgat atttctatga tacccggatg ggatattgat cagaatataa catgcacaaa
     3121 cgaaggggta aggttttcat taaccaagag agatggatta ttgttatggt ttgatgcaac
     3181 cagtaaatct tttcatgata atccagctat atctgtatca gatagtcaag caacagtgac
     3241 ttggagtttt gacagtcttg tttattatga aaatggatca ttagatccta aaaaccttct
     3301 taa
//
LOCUS       DAZGJT010000219         3277 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZGJT010000219 DAZGJT010000000
VERSION     DAZGJT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.224"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(698..1298)
                     /locus_tag="ACHKFK_004795"
                     /pseudo
     CDS             complement(698..1298)
                     /locus_tag="ACHKFK_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012016515.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
     gene            complement(1387..1821)
                     /gene="uspF"
                     /locus_tag="ACHKFK_004796"
     CDS             complement(1387..1821)
                     /gene="uspF"
                     /locus_tag="ACHKFK_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO9054937.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1962..3095)
                     /gene="ompN"
                     /locus_tag="ACHKFK_004797"
     CDS             complement(1962..3095)
                     /gene="ompN"
                     /locus_tag="ACHKFK_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO9054938.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSTNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          975 a          637 c          719 g          946 t
ORIGIN      
        1 ctaaccttca gtctgaagag ggcttgaatc gtgatgtcag ttcgattgac ataatgttat
       61 taataatgtt tacgtttgaa tgtgaaatat gcttaactat gccttgattg ttaatcacag
      121 ggactgacta tgagaaaaat actgatgtat ttatgtatta gcatatggat tgttatatca
      181 tgcgtgccta tcatattaac tagcgagtat ttaacagagg cgagtataat ggcattgatg
      241 tttggtcatg ccaatgcact atgtattggg gtttttattt ctattattta tatagaatgc
      301 tggaggtaaa tagtgtgaac agcattagtt tgatgatttt atactgaact aaagggggaa
      361 agaggtaacg aaaaaagacc tccaccaggt acgaatctga ggcgggcaga tggaggtcga
      421 tatctacacg attaaattga taagtaaaag tcccatcagg gattgatttg ttcgagtcta
      481 atttaaagta atttcaacga cttggcagaa tttcttagag ttaatgattt tcaagttttc
      541 gattaccagg aaaaaggctg ggtgctgata aaatagtcat ctgcattcct taaattgtgt
      601 aagggtgaaa ggataaagtt caatttaaag gcggtgcaat attagcacca tcttatatat
      661 gtaatgattg atttgcctct ttattctact gttgagatta ttgtttccga aaattgatta
      721 ttataagcgc gaaaagaaat gctacagcaa ataagaggac gattgtcggc gctggggctc
      781 tatcgaaaaa atataataac catacacctg atattgttat tgatatcgtt acggcgataa
      841 gtaatgcctt atgaaagcga tgcgtgatga ggacagcaat agaccctgga gctattaata
      901 gtatatagac agaataattt cgactgcttt taatgtggca aaaatggtag gcgataccat
      961 gcagagtaaa ccgtaatgta ataatgatat ctgtaatccg ctgacttgtg cctgttgata
     1021 gtcaagacaa aaattcctga aaaaacgata cctataatgg tatctttctt tatgatgcta
     1081 ttatctttca gatagctagt tgcaattgca caagacagtc ctacaataaa tgcaccaatt
     1141 cccagcggca tacctataat ccaggctaga actatgccag gaaacattgc gtggttcatt
     1201 gcatctccta ttagcgccca gccttttaat accaggaaaa ctgataataa tgcacagggg
     1261 aacgatatta taatagcgat tatcaatgca taattcataa atttaaattg taatgattag
     1321 aggggaaaat gtcagcacat ttgcgaaatg aatcaaaaag cccgcagcaa tgtgcgggcg
     1381 ttagtgtcag cgcacaacca gcacggagca ctctgcgtga cgcactacag ctgcggcgtt
     1441 agaaccgagc agataagtgg tgatatccgg tcgatgggaa gcaatgatga tcatatgagc
     1501 ggggatcttc ttcgccaatt ccagaatgcg gtctttgggc gagccttctt caacatggac
     1561 atgcactctg tcggttggca gtttaaattt tttaatgatc tcttccagtt gcgatttggc
     1621 ttccgctttc agatcatcca ttgccggtaa ttctgcggaa taggctaaac ccagagaggc
     1681 atagtagggc agcgaaggta ttaccgtcag gaaatgaacc tctgcatcat caatctttgc
     1741 ctctgcctca acgtggctaa tcacgcgttg agttaattct gaatcggaaa tatcgatagg
     1801 gacaagaatc gttctgttca taaaacctcc cgttttagta tccgcataaa gtgtaacgcc
     1861 agatgacact ttttgtgtaa tgacggagtt cacattttta atttagatca aaggaggaag
     1921 aataagcaga aaaagcccgc caaaacagcg ggcaggggga tttagaactg ataaaccaga
     1981 cctaaagcga cgatatcatc ggtagagatg ccgttggcag cgtagaaact gtcatcttca
     2041 tccaacaggt tgattttata gtcaacatag gtggacatgt ttttattgaa atagtaagtc
     2101 gcgccaacat cggcgtattt aaccagatct ttatcatcaa cacctgccgg gttgtctgca
     2161 ccaccggcag cgtgcaggtc acggccttta gacatcagga aagagactgc cggacgcaga
     2221 ccaaaatcaa actggtactg tgcagtgact tcaaagttct gggttttgtt tgcaacagca
     2281 taatcgctgt cgccaaacgg ggtcatatta cgcgtttctg aatacatggt tgccaggtaa
     2341 atattgttag catcgtattt tagcccagca gtccacgcgt ctgctttatc accacctgcc
     2401 gcagtatggt taacctggtc attggtgcgg tcagaagagg tgtatgccgc accagcgcta
     2461 aagcccatgc ctaaatcata tgttgtggaa agaccccagc cgtcaccgtt ttcatggcga
     2521 acatcacgtc cgttgttggt gccttcctga ccattactgg caccttcgtt gttaccttga
     2581 tactgcaccg cgaagttcag accatttacc agaccgaaga aatcagtatt acgataagtc
     2641 gcgacgccat tggcacgacc agtcataaag ttgtctgcat tggtataaga gtcaccgcca
     2701 aattcaggta gcatatcagt ccagccttcg atgtcgtaca ttacgccata attacgtccg
     2761 taatcgaaag aaccgtaatc tgcaaatttc agtccggcaa atgccagacg ggtccatgac
     2821 tggtttgttg aagattcggt gttgtttgcc tgaatattgt attcccattg accgtagcca
     2881 gtgagttgat cgttaatttg ggtttcgcct ttaaaaccca gacgagcata gctctggtcg
     2941 ccatctttcg ctgaattatc agaaaaataa tgcaggccat caactttgcc atacagatct
     3001 aatttgttgc cgtctttatt ataaacttcg gctgcatgtg cagcacctgc agcgagcagg
     3061 gcaggaatta agagtgccag tactttgctt ttcattgaat aaatccttta gttattttat
     3121 ttgcctttca tccccatttt ggggagtgaa aacatcctaa aggagaagtt cagttagccg
     3181 ataaatatca tttgttacgc cttaagtaaa accttaatta aataattctt cagaaatgat
     3241 aataagtctg gtgaatgtat tggagataac acataca
//
LOCUS       DAZGJT010000220         3262 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZGJT010000220 DAZGJT010000000
VERSION     DAZGJT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.225"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..505
                     /locus_tag="ACHKFK_004798"
     CDS             <1..505
                     /locus_tag="ACHKFK_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000265609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9054939.1"
                     /translation="EEETDWLPFTATPTDEAPHGKELWQALNSGQYGQIAPYTQPEDA
                     VEKARQQKINEINAWRNAMEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGILPEG
                     FFWTDAENNDVPMTAGELMALSEAAEKAMFTKGMEIHVRQRNMKKALEALNSADEILA
                     YKVGQES"
     gene            675..935
                     /locus_tag="ACHKFK_004799"
                     /pseudo
     CDS             675..935
                     /locus_tag="ACHKFK_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(945..2096)
                     /locus_tag="ACHKFK_004800"
     CDS             complement(945..2096)
                     /locus_tag="ACHKFK_004800"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFO9054940.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRSREALHHLNIQHLRRSHSLRHGRRHTRKGERGTINIVNGTPI
                     HERSRNIDNRRSLGHWEGDLVSSTKNSHIATLVDRKSRYTIILRLRGKDSVSVNQALT
                     DKFLSLPSELRKSLTWDRGMELARHLEFTVSTGVKVYFCDPQSPWQRGTNENTNGLIR
                     QYFPKKTCLAQYTQHELDLVAAQLNNRPRKTLKFKTPKEIIERGVALTD"
     gene            2158..3054
                     /locus_tag="ACHKFK_004801"
                     /pseudo
     CDS             2158..3054
                     /locus_tag="ACHKFK_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
BASE COUNT          879 a          744 c          816 g          823 t
ORIGIN      
        1 cgaggaggag acggactggc ttccgtttac ggcgacacca acggacgaag cccctcatgg
       61 aaaagaactc tggcaggcac tgaactcagg tcaatatggt caaatcgcgc cgtatactca
      121 accggaagat gcggtggaaa aagccagaca acagaaaatt aatgagatca acgcctggcg
      181 taatgcgatg gaagcggcga actacacgtt tgagcacaat gggcgtaaat gggactacgg
      241 gaagtcaacg cagacgcgtc ttgagccgtc agtggctgcc gcgaaagcag gaatactgcc
      301 ggagggtttt ttctggacgg atgcggagaa taacgacgtc ccgatgacgg ctggggagct
      361 gatggcactc agtgaggcgg cggagaaggc gatgttcacc aaagggatgg aaattcacgt
      421 acggcagcgg aacatgaaga aggcactgga agcgctgaac agtgcggatg agattctggc
      481 gtataaggtg ggtcaggagt cgtaaataaa aaaaacgggg gtgatgcctc accccctctt
      541 tcgtcaatag aggattacag caatcgtttt tttattgtga aacagattct gcaataaccg
      601 ccaggcatat tgaggtagcc tgagtttaac ggacactcct tcctgaaata gaatggcatc
      661 agaaggagct aataatgagc agaaaaaccc aacgttactc taaagagttc aaagccgaag
      721 ctgtcagaac ggttcttgaa aatcaacttt cgatcagtga aggcgcttcc cgattatctc
      781 ttcctgaagg cactttagga caatgggtta ccgccgccag aaaagggctc ggtactcctg
      841 gttcccgcac ggtggctgaa ctggaatctg aaattctgca actgcgtaag gcgttaaatg
      901 aagctcgcct tgagcgagat atattaaaaa aagcaactgt agattcaatc tgtcaatgca
      961 acaccccttt caattatctc tttcggtgtt ttgaacttca gtgtctttct cggtctgttg
     1021 tttagctgag cagcaaccag atctagttca tgttgagtat attgggcaag acatgtcttt
     1081 ttaggaaagt actgccgaat tagcccattt gtgttctcat ttgttccccg ctgccaagga
     1141 ctctgaggat cgcagaagta aactttaacg ccggtgctga cagtaaattc tagatgtctg
     1201 gccagttcca ttcctctgtc ccatgtcagt gattttctga gttctgacgg taaactcagg
     1261 aatttgtcgg taagagcctg atttactgag acagaatctt tgcccctgag tctaaggatg
     1321 atcgtataac gtgattttcg gtctacaagt gtggctatat gagagttttt tgtacttgag
     1381 actaaatcgc cctcccaatg ccccagagag cgtctgttat cgatatttcg ggaacgttcg
     1441 tgaattggtg ttccgttcac tatgttaatc gtacctcttt cgcctttgcg ggtatgacgc
     1501 ctgccatggc gaaggctatg cgaccgtcgc agatgctgta tattcaggtg gtgtagcgct
     1561 tcacggctac gaaagtacag cgttttataa attgtctcag gtgatattcg cagcgttttt
     1621 tgacgtggtt ttgttcgcct taaccatcct gatatttgct ctggagacca tttcatctcc
     1681 agcttttcca gaacaagctt tcgcaatggt aaattttgat ccagtaagca cggttttggc
     1741 cttttcgcca ttctgttggc tcggttatta gcatcaacag ctttgtaata gcgtctgccc
     1801 cgattacgct gaacttcacg tgagatcgtc gaaggactgc gattcagcgc agtagctatc
     1861 gcacgaatgc tcattttggc tgacaaacca gctcgtatct cctcgcgctc agacagtgtc
     1921 aggtgagcta cagcccgctt acgctcatgg ggttttatgc cgccagtatc ccttaacata
     1981 gtgaagatcg ttccgggttt tgaacccagg atattcgcta tttcactgaa gcctgttccg
     2041 ttcttccata gttcaaaaac agaggctttt tcctctgctg taaatgttcg tctcattcaa
     2101 aaaacctccg caaccccatg ttttcacata actgttgcgt tgaccaattg aatctacaac
     2161 agcgtatttt gcacaggagt cgctgaaaaa tacgcgttaa tcgaacaatg gcgacaacaa
     2221 tttcccattg aagcgatgtg tcaggtattt ggtgtatcca ggagcggtta ttacaactgg
     2281 gtacagcatg aaccctcaga cagaaaacaa agtgatgagc ggctaaaact ggagattaag
     2341 gtggcacata tccgcactcg cgaaacatat ggaacccggc ggctccagac ggagctggca
     2401 gagaatggca tcatcgttgg tcgtgaccga ctggcacgtc ttcgtaagga gctaaggcta
     2461 cgctgtaagc agaaacgcaa gttcagagcg actacgaacc cgaaccacaa tctgccagtt
     2521 gcgccaaatc tgctgaacca gacgttcgct cctacagcac caaatcaggt ctgggtggcg
     2581 gacctgacgt atgttgccac acaggaggga tggttgtacc tcgctggcat caaagatgtt
     2641 tatacgtgcg aaattgtcgg ctacgccatg ggagagcgca tgacaaaaga gctgacaggt
     2701 aaagccctgt ttatggcgct caggagccag cgcccacctg ccgggctaat ccaccactct
     2761 gatcgaggtt cacagtactg cgcatacgat taccgggtca tacaggagca gtttggtctg
     2821 aaaacatcaa tgtcgcgtaa aggtaactgt tacgacaacg ctccgatgga aagcttctgg
     2881 ggaacgctga aaaatgagag cctgagctac tatcgtttta ataaccggga tgaagccatc
     2941 tcagtaatac gggaatacat tgagattttc tacaatcgtc agcgtcgtca ctctcgtctg
     3001 gggaatatct ccccggcagc cttcagggaa aaatatcatc agatgactgc ttaaaaaaag
     3061 aacaaatggt agtgtccgct attgccagta cacctcatat tcagtgatgc ctgtcacacg
     3121 ctccggaacg cggatggtag cggcatatca gaaaacggtt aatattatta atgtatcgac
     3181 aaaaaatcaa aatgtggtgg ttatcacatt attaagcgta aggaactgtg tcataagtgc
     3241 gtgagggaaa taagcgaaac ga
//
LOCUS       DAZGJT010000221         3254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZGJT010000221 DAZGJT010000000
VERSION     DAZGJT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.226"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHKFK_004802"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHKFK_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO9054941.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQDG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHKFK_004803"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHKFK_004803"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO9054942.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQVSGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLTHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDRKEAFINVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="ACHKFK_004804"
     CDS             1253..1522
                     /locus_tag="ACHKFK_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO9054943.1"
                     /translation="MKCKRLNEVIELLQPAWQKESDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="ACHKFK_004805"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="ACHKFK_004805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO9054944.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEHGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQLALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAVQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHKFK_004806"
     CDS             2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHKFK_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO9054945.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKELTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          828 a          787 c          791 g          848 t
ORIGIN      
        1 gaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgct ggatgcgacg
       61 ctggcgcgtt tgctccagcc tactcttgct tatccgtcct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta acgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc cggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaaatgtag atccagctct
      361 tcttcgtctg gtgtttccgt catcaaggcc catcgctgtt ggctggcaac gatggtttgc
      421 gccgcgccag ccttgcgcaa ctcatagcta tctttgcccg gtttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcaat
      541 tttttcagca gcgttgtttt tccggtgcca ctccatgcag caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgtttata aatgcctctt
      661 tacgatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc ataacccggc
      721 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      841 gagtaagccg ggcggctaaa tcatggggaa tgtaaggcgt atcgcacggg caaaacaaaa
      901 accactcacc cgcttcctgc tgcattactg aaagcattcc tgccagaggg ccagggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttacctg atagatttcc tgatgacgat
     1021 tagcattaac cacgacgtga gaaagctgcg tcataagcgc gtcagcgaca tgctgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcccgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agtcagacct
     1321 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca cctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagatttc aaaacggcac tattacgcgc
     1501 acgtggcgta attaaagagt aaaagcttgt aagcggcgca accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg acgctctgtt tgagcatggg attcgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaacttgcg ttgcagctgg ttaacgatga agttccggtc gcagcacctg
     1861 tggcctttaa tggtcagact ttattgaacc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2041 gtttgaatga atatctcatt gagccacgca agctgtttga ggacgctaca ctaatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gattgccgcc gttacagcac
     2161 actggcggga agatttcacc gttctgcgac tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagccgttc
     2281 aggatttgtg gatgttgctc aatggtgata aagccgagca gcggatgcaa ctggaaacta
     2341 ttattgaggc ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttc ccgtggttaa ccggggaaga ttactggcta cgacagacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccagttgctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aacgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg gggggtgacc tgggcaaaga gctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tcacagttcc gctgtttgaa
     2941 ggcgtacaaa aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggtgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggtatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac tgtgaaatat ctgtccgaga aaaaataatt caatgtaaat
     3241 tacataaagc ccgt
//
LOCUS       DAZGJT010000222         3178 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZGJT010000222 DAZGJT010000000
VERSION     DAZGJT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.227"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..446
                     /locus_tag="ACHKFK_004807"
     CDS             66..446
                     /locus_tag="ACHKFK_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9054946.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVR
                     QQRLKLSLSGRLVIRIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     GQPVSGGRLGVKIYPIMLEGQVKK"
     gene            443..1263
                     /locus_tag="ACHKFK_004808"
                     /pseudo
     CDS             443..1263
                     /locus_tag="ACHKFK_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            1490..1687
                     /locus_tag="ACHKFK_004809"
     CDS             1490..1687
                     /locus_tag="ACHKFK_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO9054947.1"
                     /translation="MLKQQDMTETARVVFNELSATEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            1838..2896
                     /locus_tag="ACHKFK_004810"
     CDS             1838..2896
                     /locus_tag="ACHKFK_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9054948.1"
                     /translation="MLSTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHSQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSASQWQLPDESDYLKLQALFARVA
                     EDKHQRGELEKPHHQLVSTYSELNRQYTELQSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAIALGRRATGVELET
                     ERFEQTVREVQNLVSQNG"
     gene            2937..3012
                     /locus_tag="ACHKFK_004811"
     tRNA            2937..3012
                     /locus_tag="ACHKFK_004811"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2970..2972,aa:Ile,seq:cat)
     gene            3022..3098
                     /locus_tag="ACHKFK_004812"
     tRNA            3022..3098
                     /locus_tag="ACHKFK_004812"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3056..3058,aa:Arg,seq:tcg)
BASE COUNT          817 a          692 c          911 g          758 t
ORIGIN      
        1 tgttgttgct gattcgtttt ttaatgcacg cgagaaattc ggatgttctg aagtggaaag
       61 cataaatgac cgaacgcata gaatttgttt tgccttaccc gccgacggtg aacacttact
      121 ggcgacgccg tggcagcaca tattttatct cggaggaggg aaagcgttat cgccgggctg
      181 tggcgcttat tgttcgccag cagcggctga aattaagcct gtccggaagg ctggtaatca
      241 ggattattgc agagccaccg gataagcgtc gtcgtgacct ggacaatatt ctgaaagcac
      301 cgctggatgc gctgacgcac gcggggttgt taatggacga tgagcagttt gatgaaatca
      361 atattgtacg tggccagcca gtatctggtg gacgtctggg ggtgaagatt taccccataa
      421 tgcttgaagg gcaggtcaaa aaatgaaact ggaagattta ccgaaatact actccccaaa
      481 atcccccggc ctgactgatg catcggcctc aacgtcgaaa gatgcgctga gtatcactga
      541 tgtgatggcc gcgcagggca tgacacagaa tcgggctgag atggggtttt ctgcgttcct
      601 tgggaaaatg ggcattagta tgaatgacag agagcaggca acagaattgc tgacagaata
      661 tgcactcagt cggtgtgatc gcgtggcggc gttaagaaaa ctcccggcag aaataaaacc
      721 ggcagtgatg cgtattatgg cttcgtatgc gtttgaagat tatgcccgta gcgcggcgag
      781 caaaaaacag tgcccctgct gtcacggaaa aaaatttatt gaaagcgagg tttttacaaa
      841 caagatccag tatccggatg gtaagccgcc agtgtgggca aagtgcacaa aaggcgtgta
      901 tccgtcttac tgggaggaat ggaaaaaagt cagggaggtg gtaaaagttg cctgtccgga
      961 gtgtggaggg aagggagagg tatccactgc ctgtaaagat tgtcgtgggc gcggtgttgc
     1021 cattcatcgt gaagagtcgg aaaaacaggg tgtgccggtt ttcagaaact gtcagcgttg
     1081 tggtgggcgt ggctatgaaa gattaccttc aacggaggca tttaatgcca tatgtaatgt
     1141 aaccgatgcc atatctcttg atacatggaa aaaacagtta aacgttttta cgatacgctg
     1201 gtggtgcagt ttgatattga agaagcatgg gcagaacaac aactgaaaaa ggtgaccaga
     1261 tagctttgtt gatttttccc gaatctgtgg taaatttgcc ctaacgatgg gcgttttatg
     1321 cctgacgtta gaagattttt tacaccccgc cgcctggcgg gttttttatg actgaaatcg
     1381 cgtcagtaca gtaaacgcgc tggtggcggt gaatacctgt ctttcagctt gctggctttt
     1441 tcgacaagag ttattggtgt gtcacgttaa ccggaaaagg gaaaaagaca tgctgaaaca
     1501 gcaggatatg accgaaaccg ccagagtggt gtttaatgaa ttaagtgcca ccgaaccggc
     1561 gacagtcggg gagattgcgc agaatactta cctttcacgt gaacgctgcc agttaatact
     1621 gacccagctt gttatggcgg ggctggcaga ctatcagttc ggttgttaca gacgccttca
     1681 gtcctgaagg cttttttatt tgtggtaaat gggcggctgg tgggtgttag gggcactcac
     1741 cagccatctg ctcatgcgtc cggatcacaa gcaaacctca ggcccactgc tttgcgcaaa
     1801 agcagaatga gcctatcaga gacaggctta atgatccatg cttagtactg taaaaatatc
     1861 cagttgtgag ttaatcaacg ccgactgcct ggaatttatc cggtcgttac ccgaaaattc
     1921 tgttgacctg atagtcacgg acccgccgta ctttaaagtg aagcctgagg gctgggataa
     1981 ccagtggaag ggcgacgatg attacctgaa gtggctggac cagtgtcttg cgcagttctg
     2041 gcgggtgctg aaacctgccg gaagtcttta cctgttctgt ggccatcgcc tggcgtctga
     2101 cattgaaatc atgatgcgtg aacgcttcaa tgtgctgaac catattatct gggcgaagcc
     2161 gtccggacgc tggaacgggt gcaacaagga aagcctgagg gcatatttcc ccgccacaga
     2221 gcgcattctg ttcgcggaac attctcaggg gccgtatcgc ccgaaagatg ccgggtatga
     2281 ggcgaagggc agggcactga aacagcatgt gatggccccg ctgatttctt actttcgtga
     2341 tgcgcgtgct gccctgggga taacggcaaa acagattgct gatgccacag gaaagaaaaa
     2401 catggtgtcg cactggttca gtgccagcca gtggcagcta ccggacgaaa gcgattatct
     2461 gaaattacag gcgctgtttg cccgggtggc agaagataag catcagcgcg gagaactgga
     2521 aaagccacat caccaactgg tcagcacata cagtgaactg aaccggcagt atacggaact
     2581 gcagagtgaa tataagcatc tgcggcggta ttttggtgtg acggtgcagg tgccgtacac
     2641 cgatgtgtgg acgcataaac cggtgcagta ctatcccggg aaacatccgt gcgaaaaacc
     2701 ggcagaaatg ctgcagcaga taatcagcgc gagcagtcgt ccgggtgacc tggttgcaga
     2761 tttcttcatg gggtcaggtt cgacagtcaa agcagcgata gcgctgggac gtcgtgcaac
     2821 tggcgttgag ctggagactg agcgttttga gcagacggtc agggaagttc agaatttagt
     2881 cagtcagaac ggatgatatt gcagaattag ttacgtaccg ttattatcct atgcccggcc
     2941 ctttagctca gtggtgagag cgagcgactc ataatcgcca ggtcgctggt tcaaatccag
     3001 caagggccac catatcacat accgccatta gctcatcggg atagagcgcc agccttcgaa
     3061 gctggctgcg cggggttcga gtcctcgatg gcggtccatt atctgcatta tgcgttgttg
     3121 gttctgatga gtgaatcatt gtcttttgag caacaggcta tcatataaga tagcctca
//
LOCUS       DAZGJT010000223         3090 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZGJT010000223 DAZGJT010000000
VERSION     DAZGJT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.228"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            560..1513
                     /gene="ompT"
                     /locus_tag="ACHKFK_004813"
     CDS             560..1513
                     /gene="ompT"
                     /locus_tag="ACHKFK_004813"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO9054949.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETLSFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKIPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1745..2413
                     /locus_tag="ACHKFK_004814"
     CDS             1745..2413
                     /locus_tag="ACHKFK_004814"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9054950.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFDPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVKGTVVMFSA
                     SGKK"
     gene            complement(2468..2837)
                     /locus_tag="ACHKFK_004815"
                     /pseudo
     CDS             complement(2468..2837)
                     /locus_tag="ACHKFK_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2844..3090
                     /locus_tag="ACHKFK_004816"
                     /pseudo
     CDS             2844..3090
                     /locus_tag="ACHKFK_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          981 a          679 c          631 g          799 t
ORIGIN      
        1 tgttttcatt ttattcgtct gacgtgttcc aattatttag caaaactgcg acaacaaagt
       61 gatggtgtaa tttgtaaaag ggaacaaata aatggctcgc cctgcaggat tcgaacctgt
      121 agcccacggc ttagaaactc caggaacgac attttaagtc aacaaattac cgcgccatct
      181 ctgcgttcac acgtcccact acctcaaaat atgtaaaacc ttgcaagcca ttgcgaggcc
      241 ttatgtgtct cagttttgtt cctctttttt atactaaaaa acacagcaat tgaggataaa
      301 cctcatgcta ttatcgctaa tatgcatcta aaggcatggc acttgaatag ataaaaactc
      361 cacaaagaaa aaataattag cccataaaac cacaatgcaa aacacaaaca gacaattaaa
      421 ctaaaaaact gacagtgcat tgtgacaaaa attcaatgtt aaacaaaata taaacagtgg
      481 ggcaatatac aattgactca ttaagttaga tataaaaaat acatactcaa tcattaaaac
      541 gattgaatgg agacctttta tgcgggcgaa acttctggga atagtcctga caacccctat
      601 tgcgatcagc tcttttgctt ctaccgagac tttatcgttt actcctgaca acataaatgc
      661 ggacattagt cttggaactc tgagcggaaa aacaaaagag cgtgtttatc tggccgaaga
      721 aggaggccga aaagtcagtc aacttgactg gaaattcaat aacgctgcaa ttattaaagg
      781 ggcaattaat tgggatttga tgccccagat atctatcggg gctgctggct ggacaacact
      841 cggcagccga ggtggcaata tggtcgatca ggactggatg gattccagta accccggaac
      901 ctggacggat gaaagtagac accctgatac acaactcaat tatgccaacg aatttgatct
      961 gaatatcaaa ggctggctcc tcaacgaacc caattaccgc ctgggactca tggccggata
     1021 tcaggaaagc cgttatagct ttacagccag aggtggttcc tatatctaca gttctgagga
     1081 gggattcaga gatgatatcg gctccttccc gaatggagaa agagcaatcg gctacaaaca
     1141 acgttttaaa ataccctaca ttggcttgac tggaagttat cgttatgaag attttgagct
     1201 aggtggcaca tttaaataca gcggctgggt ggaagcatct gataacgatg agcactatga
     1261 cccaggaaaa agaatcactt atcgcagtaa agtcaaagac caaaattact attctgttgc
     1321 agtcaatgca ggttattacg taacacctaa cgcaaaagtt tatgttgaag gcgcatggaa
     1381 tcgggttacg aataaaaaag gtaatacttc actttatgat cacaatgata acacttctga
     1441 ctacagcaaa aatggtgcag gcatagaaaa ctataacttc atcactactg ctggtcttaa
     1501 gtacaccttt taacaacgtt aaatagattt tctccccggt ggtattgtat agccccgggg
     1561 aatttgttta ccgttaaaag caaacaatat cccctaatct tgtaagcatt taacctgcgt
     1621 cctggcgtgt aacctcttac cgcaacacaa gactcaatat attcaaattc gttatgccgt
     1681 ggcatcattt aaccccttgt aattcatcgc tataattgat ttaattcaca aataaaacta
     1741 taacatggtg aaactaatga aaaaaaacac agatgatggg gctaaaattt acacaccact
     1801 taccctaaag ctttatgact ggtgggtttt gggtgtatca aatcggcttg catggggatg
     1861 tcctacaaag gaacaccttc ttccacactt tctggaacat ttaggtaaca accatctgga
     1921 tattggcgtt ggaactgggt tttaccttac tcacgtacct gagagtagtc tgatatcttt
     1981 aatggatttg aacgaagcta gcctgaacgc ggcatcgaca agggctgggg aatcaaaaat
     2041 taaacataaa attagccatg atgtttttga tccttatccc gcggcgttac atggtcaatt
     2101 tgattccatt tccatgtttt accttcttca ctgcctgcct ggaaatatat ctacaaaaag
     2161 ctgtgtaata cgcaatgcgg cgcaggcctt aactgacgat ggaactctat acggagccac
     2221 aattcttggt gacggcgttg tgcacaatag cttcggtcaa aaactgatgc gcatttacaa
     2281 tcagaaaggc atcttttcaa acacaaaaga ttccgaagaa ggcttaacac atatactctc
     2341 agagcatttc gagaatgtta aaaccaaggt taaaggtact gtagtaatgt tttccgcttc
     2401 agggaaaaaa tagcatccag ccgcagcacg ttcttgcgca cgacgtgctg cggcataatc
     2461 ccaatgatta ctctctgaca gggttcacag gccactcaat atcaggggca gttgatgtat
     2521 caactcggtt cagcaacacc cgatacatct tccaggcttc cagcaacgag gtttcttcct
     2581 ccgtatcctt tatccatgat gtgccgttct acttctgaaa ctcccctgcc ggggatacca
     2641 ggtgacattt tccggtaacg gaccgagttc agaaataaat aatgcgtccc ctgatgccac
     2701 gtcataaacc gttttacccc gatggtcttc aacgagatgc cacgatgcct catcactgtt
     2761 gaaaacaacc acgaagccag ccggaatatc tgggcaatat cagtactgtt tgcaggcaaa
     2821 cctgtatgag gtggaatggc acaagagact tacaaaagaa tcaggcaaaa cgcctgattg
     2881 atgagagccc cgaactactg aaatctgtcg tcaaatttgc gctggtaact gttctgagaa
     2941 agtcgaacat cataaatctg gagtggcaac agattgacat gcagcgacaa gttgccaggg
     3001 tgaatccaga agatagcaaa tcaaaccgcg ccattggtgt ggcgctgaac gataccgcct
     3061 ctaaagtgtt gcgtgatcaa acaggcaagc
//
LOCUS       DAZGJT010000224         3086 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZGJT010000224 DAZGJT010000000
VERSION     DAZGJT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.229"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1296
                     /locus_tag="ACHKFK_004817"
     CDS             <1..1296
                     /locus_tag="ACHKFK_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO9054951.1"
                     /translation="KGDAGPRGETGPAGPRGEKGEQGERGPQGIPGQKGDTGERGPQG
                     VQGPAGATGPSGPRGATGPQGAMGPRGETGARGEKGDPGGPQGPKGDTGPRGEPGPAG
                     AKGERGETGPAGPKGEPGERGPQGLQGPAGERGEPGPAGPKGERGEAGPQGPRGEPGP
                     AGSAANVADATTAQKGIVQLSSATDSDDETKAATPKAVKAAMDEARAAKQKAEEVATT
                     RPSGESIPGPKGDRGEQGPAGPTGPKGERGEPGPQGPAGQKGEQGLRGLQGATGPAGA
                     VGPAGPRGPAGAAGAKGAPGPKGDKGEPGATGPAGPQGKTGPAGPRGPAGPQGAAGRN
                     GNVSTEKYAVGSFLFGSISTLESESAIKENISGEKITEVAILYRVGVTYSPTFSIVHN
                     AGNSIPGTWRAFVSAFSLGAPGYDEQTMTTNLTLFQRIV"
     gene            1310..1882
                     /locus_tag="ACHKFK_004818"
     CDS             1310..1882
                     /locus_tag="ACHKFK_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9054952.1"
                     /translation="MAIVVTDVRGAVYTRLGNISCEIKIGEQWFPFTASENDPENHGR
                     QLFTDLISGRYGEIKPYEEPPEALTQSRERKKVEINAWRNAMEAANYTFEHNGRKWDY
                     GKQTQARLEPSVAAAKAGILPEGFFWTDAENNDVLMTAGELMALSEAAEKAMFTKGME
                     IHVRQRNMKKALEALNSADEILAYKVGQES"
     gene            2362..>3086
                     /locus_tag="ACHKFK_004819"
     CDS             2362..>3086
                     /locus_tag="ACHKFK_004819"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054953.1"
                     /translation="MNKKFSKTILSSAVAGLLLVSGGAMAANKTKVVGKYTIEYMGDD
                     SAKIYETGKSQNDGNGNVLLVNTEKGSVSVVNEKEISDAVNAIKSSTQYAELKKKYNN
                     DNVVDKMIVQHAANIYKYAIKTPSLKLENLEKITGEEIEKIDSDVKKAKEVITSKTAT
                     AYNTAIENGVSVDSALAAAKDNNGALLNEFNRLGTNVNDLKNATTFALDADGNITLDE
                     NQGSGERYSVKQTVADIKADTTV"
BASE COUNT          916 a          624 c          946 g          600 t
ORIGIN      
        1 aaaggtgatg ctggtcctcg tggtgaaaca ggtccggcgg gtccgcgtgg ggagaaaggt
       61 gagcagggag agcgcggacc tcagggcata ccgggccaga aaggtgatac aggagaacgg
      121 ggacctcagg gagtacaggg gccagctggc gcaacagggc catctggccc acgtggtgcg
      181 accgggccac agggagcaat ggggccgcgt ggtgaaaccg gggccagagg cgaaaaagga
      241 gacccggggg ggcctcaggg accaaagggg gatactggtc cgagaggtga gccggggccg
      301 gcaggagcaa agggagagcg cggtgagacc ggacctgcgg ggccgaaagg ggaacccggg
      361 gaacgtggtc cgcaaggatt acagggcccc gccggagaga gaggtgaacc gggtccggca
      421 ggtccgaaag gtgaacgagg cgaagccgga cctcagggac ctcgtgggga gccaggcccg
      481 gcgggcagcg cagcaaatgt ggcagatgcg acgacggcac agaagggaat tgtgcagtta
      541 agcagcgcaa cggacagtga tgatgaaacg aaggcagcca caccgaaagc tgtgaaggcg
      601 gctatggatg aggccagggc agcgaaacag aaggcagaag aggttgcgac aacccgcccg
      661 tcgggagaaa gcatacctgg accgaaaggt gacaggggcg agcaaggacc ggcaggtccc
      721 acaggtccga aaggtgagcg cggtgaaccc ggacctcagg gacctgcagg acagaaaggt
      781 gagcagggcc tccgtgggct gcagggggcg acaggacctg cgggagcagt ggggcccgca
      841 ggcccacggg gaccggctgg tgcagccgga gccaaaggtg ccccggggcc aaaaggggac
      901 aaaggcgaac ccggagcaac aggtcccgcg gggccgcagg ggaaaaccgg cccggcaggt
      961 cctcgtggtc cggcagggcc gcagggggca gcaggcagga atggtaatgt atccacagaa
     1021 aaatatgcgg ttggctcttt cttatttggt tcgatatcga ctctcgaaag tgaaagtgca
     1081 attaaagaga atatcagtgg cgaaaaaatt actgaagtgg cgattcttta ccgcgttggc
     1141 gtcacgtatt cgcccacgtt ctcgattgtt cataatgccg gaaacagtat cccggggaca
     1201 tggcgggctt ttgtgtctgc tttcagtctg ggtgctcccg gatacgatga gcagaccatg
     1261 acaacaaact taacgttatt tcaacgcatt gtgtaataca gggggaagaa tggcgattgt
     1321 tgtaacagac gtcagggggg cggtatatac tcgattgggt aatatttcct gtgagataaa
     1381 aattggtgag caatggtttc cttttactgc gtcagaaaat gatccggaaa atcacggacg
     1441 acaacttttt actgatttaa tttccggcag gtatggagag attaagccgt atgaagaacc
     1501 gccggaagct ttaacacagt cacgtgaacg gaagaaggtg gaaattaatg cctggcgtaa
     1561 tgcgatggaa gcggcgaact acacctttga gcacaatggc aggaaatggg actacggcaa
     1621 acagacacag gcgcgtcttg agccgtcagt ggctgccgcg aaagcaggaa tactgccgga
     1681 gggctttttc tggacggatg cggagaataa cgacgtcctg atgacggctg gggagctgat
     1741 ggcactcagt gaggcggcgg agaaggcgat gttcaccaaa gggatggaaa ttcacgtacg
     1801 gcagcggaac atgaagaagg cactggaagc gctgaacagt gcggatgaga ttctggcgta
     1861 taaggtgggt caggagtcgt aaataaaaaa acgggggtga tgcctcaccc cctctttcgt
     1921 caatagagga ttacagcaat ccttttttta ttgtgaaaca gattctgcaa taaccgccag
     1981 gcatattcag tgatgcctgt cacacgctcc ggaacgcgga tggcagcggc atgtcagaaa
     2041 acgattaata ttattaatgc atcgaaaaaa atcagaatgt ggtgcttatc acattattaa
     2101 gcgtaaggaa ctgtgtcata agtgcgtgag ggaaataagc gaaacgacag catgcggtgt
     2161 ggagcgtgaa taatggcggg gatgacggat atcaccgact gtacagtacg ataattccta
     2221 aaaagttcat attcattttt gcgaataatc ctggaataag ctgacaggaa gcggccagtc
     2281 atcagatttt ctttagtgta tctgaagtgc gtctgagagg gttgcttttt ttgtatacaa
     2341 agaaattaca ggatatatca gatgaacaaa aaattctcta agactattct ttcttctgct
     2401 gttgccggac tgttgctggt gagtggcggg gctatggctg ctaataaaac taaagtagtt
     2461 ggaaagtata cgattgagta tatgggagat gactccgcaa aaatttatga aactggaaaa
     2521 tcccaaaacg atggaaatgg aaatgtactt ctggttaata cagaaaaagg tagtgtttcc
     2581 gttgtaaatg aaaaggaaat aagtgatgcg gtaaatgcaa ttaaatcaag tacacagtat
     2641 gcagaattaa aaaaaaaata caataacgat aatgtcgtag ataagatgat agtacagcat
     2701 gcagcaaata tatataaata tgcaattaaa acgccttctt taaaattaga aaacctagag
     2761 aaaattactg gtgaggaaat tgagaaaata gacagcgatg ttaaaaaggc gaaagaagtt
     2821 atcacatcaa aaacagctac tgcatacaat acagccatcg aaaatggcgt aagcgttgac
     2881 tcagcgcttg cggctgcgaa agacaataac ggtgcattac tgaatgagtt caaccgtctt
     2941 ggtacaaatg taaatgacct gaaaaatgcc actacatttg cccttgatgc cgatggcaat
     3001 atcacgctgg atgaaaatca gggaagcggt gagcgttatt ctgtaaaaca aacagtcgca
     3061 gatattaagg ctgacactac tgtcta
//
LOCUS       DAZGJT010000225         3047 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZGJT010000225 DAZGJT010000000
VERSION     DAZGJT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.230"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            346..636
                     /locus_tag="ACHKFK_004820"
     CDS             346..636
                     /locus_tag="ACHKFK_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO9054954.1"
                     /translation="MSTAASVRKIPRENQINIRATDEERAVIDYAASLVNKNRTDFII
                     ELAYQEAKNIILDQRLFVLDNEGYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            627..1109
                     /locus_tag="ACHKFK_004821"
     CDS             627..1109
                     /locus_tag="ACHKFK_004821"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9054955.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQILNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRIANLAEQVGIKAVMVHAIDDDARVFYEHFGFVQSVVAPDTLFYKI
                     "
     gene            1394..1651
                     /locus_tag="ACHKFK_004822"
     CDS             1394..1651
                     /locus_tag="ACHKFK_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO9054956.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGKPLSEAERQRAASARKRSVCKEI
                     KVFVRPELKNYLTSLCAEEGVTQAELIERLIEKEACHRNMM"
     gene            complement(1687..1920)
                     /locus_tag="ACHKFK_004823"
     CDS             complement(1687..1920)
                     /locus_tag="ACHKFK_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HFO9054957.1"
                     /translation="MSTERSLELFRAYNYTPRIEQQLLCGFLLAPVIFSVAKLAEDYR
                     GFHFSGSCKSTSEPVPCHLTAWPATKFLKRSVI"
     gene            1911..1958
                     /locus_tag="ACHKFK_004824"
     CDS             1911..1958
                     /locus_tag="ACHKFK_004824"
                     /inference="COORDINATES: protein motif:HMM:TIGR03475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054958.1"
                     /translation="MCSLLLCNISAGRCD"
     gene            1951..2808
                     /gene="repA"
                     /locus_tag="ACHKFK_004825"
     CDS             1951..2808
                     /gene="repA"
                     /locus_tag="ACHKFK_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO9054959.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSVLFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDAGRERQDIVTLVKRQLTREIAEGRFTANGEAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          768 a          709 c          782 g          788 t
ORIGIN      
        1 aagcacggaa gtaagctgcc gggcggggac ggaagtcccc gctttccgga agtgtgaggt
       61 atttcagggg cagacacccg acatgccaga aacagccggt cccgtccggg gccggccccc
      121 gggttaaggc atttcctgct tttcagtcat ttcattatca aaatcacatt aaacggtcgt
      181 aatcagacat gatttgtgcc ccaacacaga tcattgtcac aattttcaag tctctgtcat
      241 tgtttttatc agggattatt tttcctgcaa gtgatttcgt atagcgttct gttaccattt
      301 gcgataacat tcattgtata gcaaatcgtt accaatggag gttttatgtc cactgcagca
      361 agcgtccgta aaataccgcg agaaaatcag attaacatcc gggcaactga tgaggaaaga
      421 gccgttattg attatgctgc gagtctggtc aataagaatc gtaccgattt catcattgaa
      481 ctggcttacc aggaagctaa aaacatcatt ctggatcaac gtttatttgt attggataac
      541 gaaggttatg attcgtttat tacgcaactg gaagctcctg tacagaatgc agaagggcgt
      601 gaacgtctga tggcggtaaa gcctgaatgg aaataaatgt caccgcgccg gcattgctga
      661 cagatgagca tatacttcag ccatttgact gtgggaatga ggtgttaagt aactggttgc
      721 gtggtcgggc tatgaaaaac cagatactca atgcttctcg cacgttcgtc atttgtctgg
      781 aggatacttt acgtgttgtg gggtattact cattagctac tggttctgtc acacatgccg
      841 agcttgggcg cagtttgcgc cataatatgc ccaatcctgt acctgttgtt cttttagggc
      901 gtctggctgt cgatgtttgt actcagggac atggtttcgg taaatggctt ctgagtgatg
      961 ccattcatcg gattgccaat ctggctgaac aggttggcat taaggctgtt atggttcatg
     1021 caattgatga tgatgccaga gttttttatg agcattttgg ttttgttcag tcagttgtgg
     1081 cacctgatac cttgttttat aaaatttgat gttacgcaat tttattgggc gtagctttat
     1141 aatgatgtaa atcgatagtc cctcggacta gctttcacat ttcagaaatc gccgatcccg
     1201 cccggggccg gcacacacgt ttagttattt tctgtttttt cgtcatttca ttatcaaaat
     1261 cacattaaac ggtcgtaatc aggcatgatt tgtgcgccaa cacagatcat cgtcacaact
     1321 ttcaagtcgc tgatttaaaa aaactgtagt atcctctgcg aaacgatccc tgtttgagaa
     1381 ttgaggaggc gagatgtcgc agacagaaaa tgcagtgact tcctcatctg gcgcaaaacg
     1441 agcatacaga aagggtaaac ctttgagtga agcagagcgg cagagggctg cttctgcgcg
     1501 caaacgatct gtatgtaaag agattaaggt tttcgtcagg ccagaactga aaaactacct
     1561 taccagtctg tgtgcagagg aaggggtaac tcaggcagag ttgatagaaa ggcttatcga
     1621 gaaagaagcc tgtcaccgga atatgatgtg atgggatcac attcttgcta gttttttctg
     1681 tcaatgctag attactgagc gttttaggaa ttttgtggct ggccacgccg taaggtggca
     1741 gggaactggt tctgatgtgg atttacagga gccagaaaag tgaaaacccc gataatcttc
     1801 agcaagtttg gcgactgaga agattaccgg ggctaacaag aaaccgcata gaagctgttg
     1861 ctctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt ctgtgctcac
     1921 tccttctgtg caacataagt gcaggaaggt gtgactgatc ttcaacaaac gtattaccgc
     1981 caggtaaaga acccgaatcc ggtatttaca ccccgtgaag gtgccggaac gctgaagttc
     2041 tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt cgccattcat
     2101 gtggcgcatg cccgttcgcg tggtctgcgt cggcgcatgc cgccggtact gcgtcgacgg
     2161 gctattgatg cgctcctgca ggggctgtgt ttccactatg acccgctggc caaccgcgtc
     2221 cagtgctcca tcaccacact ggccattgag tgcggactgg cgacggagtc tgctgccgga
     2281 aaactctcca tcacccgtgc cacccgggct ctgacgttcc tgtcagagct gggactgatt
     2341 acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga tatcacgttc
     2401 acatctgtac tgtttgctgc cctcgatgta tcagaggagg cagtggccgc cgcgcgccgc
     2461 agccgtgtgg aatgggaaaa cagacaacgt aaaaagcagg ggctggatac cctgggtatg
     2521 gatgaactga tagcgaaagc ctggcgtttt gtccgtgagc gtttccgcag ttaccagaca
     2581 gagcttaagt cccggggaat aaagcgtgcc cgtgcgcgtc gtgatgcggg cagggaacgt
     2641 caggatatcg tcaccctggt gaaacggcag ctgacgcgtg aaatcgcgga agggcgcttc
     2701 actgctaatg gtgaggcggt aaaacgcgaa gtggagcgtc gtgtgaagga gcgcatgatt
     2761 ctgtcacgta accgcaatta cagtcggctg gccacagctt ccccctgaaa gtgacctcct
     2821 cagaataatc cggcccgcgc cggaggcatc cgcacgcctg aagtccgtca gcgcacaaaa
     2881 aatcagcacc acatacaaaa aacaacctca ccatccacct tctggagcat ccggttcccc
     2941 ctgtttttaa tacaaaacac gcctcacaga cggggaattt tgcttatcca catttaactg
     3001 caagggactt ccccataagg ttacaaccgt tcatgtcata aagcgcc
//
LOCUS       DAZGJT010000226         2870 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZGJT010000226 DAZGJT010000000
VERSION     DAZGJT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.231"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..2431
                     /gene="ppsA"
                     /locus_tag="ACHKFK_004826"
     CDS             53..2431
                     /gene="ppsA"
                     /locus_tag="ACHKFK_004826"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO9054960.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGGNV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2488..>2870)
                     /locus_tag="ACHKFK_004827"
     CDS             complement(2488..>2870)
                     /locus_tag="ACHKFK_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HFO9054961.1"
                     /translation="EAGYIKITRRKKDIIVRGGENISSREVEDILLQHPKIHDACVVA
                     MPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTVSG
                     KIQKFLLRKDIMRRLTQDVCEEIE"
BASE COUNT          667 a          721 c          792 g          690 t
ORIGIN      
        1 tgcgcagaaa tgtgtttctc aaaccgttca tttatcacaa aaggattgtt cgatgtccaa
       61 caatggctcg tcaccgctgg tgctttggta taaccaactc ggcatgaatg atgtagacag
      121 ggttgggggc aaaaatgcct ccctgggtga aatgattact aacctttccg gaatgggtgt
      181 ttccgttccg aatggtttcg ccacaaccgc cgacgcgttt aaccagtttc tggaccaaag
      241 cggcgtaaac cagcgcattt atgaactgct ggataaaacg gatattgacg atgttaccca
      301 gcttgcgaaa gcgggcgcgc aaatccgcca gtggattatc gacactccct tccagcctga
      361 gctggaaaac gccatccgcg aagcctatgc acagctttcc gccgatgacg aaaacgcttc
      421 gtttgcggtg cgttcctccg ctactgcaga agatatgccg gacgcttctt ttgccggtca
      481 gcaggaaact ttcctcaacg ttcagggttt tgacgccgtt ctcgtggcag tgaagcatgt
      541 atttgcttct ctgtttaacg atcgcgccat ctcttatcgt gtgcaccagg gttacgacca
      601 tcgtggcgtg gcgctctccg ccggtgttca gcggatggtg cgctccgacc tcgcatcttc
      661 tggcgtgatg ttctccattg ataccgaatc cggctttgac caggtggtgt ttatcacttc
      721 cgcatggggc cttggtgaaa tggtcgtgca gggtgcggtt aacccggatg agttttatgt
      781 gcataaaccg acacttgcgg cgaatcgccc ggctatcgtg cgccgcacca tggggtcgaa
      841 aaaaatccgc atggtttacg cgccgaccca ggagcacggc aagcaggtta aaatcgaaga
      901 cgtaccgcag gaacagcgtg acatcttctc gctgaccaac gaagaagtgc aggaactggc
      961 aaaacaggcc gtacaaattg agaaacacta cggtcgcccg atggatattg agtgggcgaa
     1021 agatggccac accggcaaac tgttcattgt gcaggcgcgt ccggaaaccg tgcgctcacg
     1081 cggtcaggtc atggagcgtt atacgctgca ttcacagggt aagattatcg ccgaaggccg
     1141 tgctatcggt catcgcatcg gcgcgggtcc ggtgaaagtc atccatgaca tcagcgaaat
     1201 gaaccgcatc gaacctggcg acgtgctggt tactgacatg accgacccgg actgggaacc
     1261 gatcatgaag aaagcatctg ccatcgtcac caaccgtggc ggtcgtacct gtcacgcggc
     1321 gatcatcgct cgtgaactcg gcattccggc ggtagtgggc tgtggtgatg caacagaacg
     1381 gatgaaagac ggcgggaacg tcactgtttc ttgtgccgaa ggtgataccg gttacgtcta
     1441 tgcggagttg ctggaattta gcgtgaaaag ctccagcgta gaaacgatgc cggacctgcc
     1501 gttgaaggtg atgatgaacg tcggtaaccc ggaccgtgct ttcgacttcg cctgcctgcc
     1561 gaacgaaggc gtgggccttg cgcgtctgga atttatcatc aaccgtatga ttggcgtcca
     1621 cccacgcgca ctgcttgagt ttgacgatca ggaaccgcag ttgcaaaacg aaatccgcga
     1681 gatgatgaaa ggttttgatt ctccgcgtga attttacgtt ggtcgtctga ctgaagggat
     1741 cgcgacgctg ggtgccgcgt tttatccgaa gcgcgtcatt gtccgtctct ctgattttaa
     1801 atcgaacgaa tatgccaatc tggtcggtgg tgagcgttac gagccagatg aagagaaccc
     1861 gatgctcggc ttccgtggcg cgggccgcta tgtttccgac agcttccgcg actgcttcgc
     1921 gctggagtgt gaagcagtga aacgtgtgcg caacgacatg gggctgacta acgttgagat
     1981 catgatcccg ttcgtgcgta ccgttgatca ggcgaaagcg gtggttgagg aactggcgcg
     2041 tcaggggctg aaacgtggtg agaacgggct gaaaatcatc atgatgtgtg aaattccgtc
     2101 caacgccttg ctggcagagc agttcctgga atatttcgac ggcttctcaa ttggctcaaa
     2161 cgacatgacg cagctggcgc tcggtctgga tcgtgactcc ggcgtggtgt ctgaactgtt
     2221 cgatgagcgc aacgatgcgg tgaaagcact gctgtcgatg gcgattcgtg ccgcgaagaa
     2281 acagggcaaa tatgtcggga tttgcggtca gggtccatcc gaccacgaag actttgctgc
     2341 atggttgatg gaagagggga tcgatagcct gtctctgaac ccggacaccg tggtgcaaac
     2401 ctggttaagc ctggctgaac tgaagaaata aaataaatac ccggcggcgt tttgtcgccg
     2461 ggtttatgtg atccccgaag atgaaactta ttcaatctct tcacagacat cctgagttaa
     2521 tcgccgcata atatctttcc ttaacaaaaa cttttgtatt ttacctgaga cagtgcgcgg
     2581 tagtttttcg attaccacga tatgttcagg atatttatat tttgcgaccc gtttacggct
     2641 aaaaaaagcc accacctctt ccagcgataa tgaatgatgc ggcgctttca gcacgacata
     2701 agcgcatgaa cgttcaccta aacgttcatc gggcatcgca accacacagg catcgtgaat
     2761 tttaggatgc tgcaataaaa tatcttctac ttcacggctg ctaatatttt cgccgccgcg
     2821 gacaataata tctttcttgc gcctggttat ttttatatag ccagcctcgg
//
LOCUS       DAZGJT010000227         2868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZGJT010000227 DAZGJT010000000
VERSION     DAZGJT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.232"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(108..184)
                     /locus_tag="ACHKFK_004828"
     tRNA            complement(108..184)
                     /locus_tag="ACHKFK_004828"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(148..150),aa:Asp,seq:gtc)
     gene            complement(317..1048)
                     /gene="dnaQ"
                     /locus_tag="ACHKFK_004829"
     CDS             complement(317..1048)
                     /gene="dnaQ"
                     /locus_tag="ACHKFK_004829"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO9054962.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNATF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGETTIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            1113..1580
                     /gene="rnhA"
                     /locus_tag="ACHKFK_004830"
     CDS             1113..1580
                     /gene="rnhA"
                     /locus_tag="ACHKFK_004830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO9054963.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGHEKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDTALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1577..2299)
                     /locus_tag="ACHKFK_004831"
     CDS             complement(1577..2299)
                     /locus_tag="ACHKFK_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO9054964.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2333..>2868
                     /gene="gloB"
                     /locus_tag="ACHKFK_004832"
     CDS             2333..>2868
                     /gene="gloB"
                     /locus_tag="ACHKFK_004832"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO9054965.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAISANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTPSQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIL"
BASE COUNT          764 a          703 c          676 g          725 t
ORIGIN      
        1 tttacagcat aacaaaatca tagaccaaag aaatagcggg atttagatga aaaaaccgca
       61 aaattaaaaa tcttgcggtt ctctgaactc attttcatga gtgaatttgg cggaacggac
      121 gggactcgaa cccgcgaccc cctgcgtgac aggcaggtat tctaaccgac tgaactaccg
      181 ctccgcgttg tgttccgttg ggaacgaggc gaatattacg gattgcctca cccttcgtca
      241 acggtttttc tcatcttttg aatcgtttgc tgcaaaaatc gcccaagtcg ctatttttag
      301 cgccttttac aggtatttat gcccgccaga ggcagcttcc gcccttcttc tgcaccagat
      361 cgagacgggc ttcatgtgct gcaagctctt catctgtcgc aaaaacaacg cgtaacttac
      421 ttgcctgacg cacaatgcgc tgaattgttg tttcaccttg ttgctgttgt gtctctcctt
      481 ccatcgcaaa agccatcgac gtttgaccac ctgtcatcgc cagataaact tccgcaagga
      541 tctgggcatc gagtaatgcc ccatgcagcg ttcgtttact gttatctatt tcgtagcggg
      601 cacataacgc atcgaggctg ttgcgtttac cgggaaacat tttcctcgcc accgcaaggc
      661 tatcggtgac cttacagaag gtattggtct tcggaatatc gcgcttaagc aacgaaaact
      721 cgtagtccat aaagccgata tcgaacgttg cgttatggat caccagctcc gctccgcgga
      781 tatagtccat gaactcatcg gctacctcgg caaacgtggg cttatcgagc aaaaattcat
      841 cggcaatacc atgtacgcca aaggcttccg gatccaccag ccgatcgggt ttgagataaa
      901 catggaagtt attgcccgtc aggcgacggt tcaccacttc aacggcacca atctcaatga
      961 tcttgtggcc ttcatagtgc gcaccaatct ggttcatacc ggtggtttcg gtatcgagaa
     1021 cgatctggcg tgtaattgca gtgctcatag cggtcattta tgtcagactt gtcgttttac
     1081 agttcgattc aattacagga agtctaccag agatgcttaa acaggtagaa attttcaccg
     1141 atggttcgtg tctgggtaat cctggacctg ggggttacgg cgctatttta cgctatcgcg
     1201 gacacgagaa aacctttagc gctggctaca cccgcaccac caacaaccgt atggagttga
     1261 tggccgctat tgtcgcgctg gaggcgttaa aagaacattg cgaagtcatt ttgagtaccg
     1321 acagccagta tgtccgccag ggtatcaccc agtggatcca taactggaaa aaacgtggct
     1381 ggaaaacagc agacaaaaaa ccagtaaaaa atgtcgatct ctggcaacgt cttgatactg
     1441 ctctggggca gcatcaaatc aaatgggaat gggttaaagg ccatgccgga cacccggaaa
     1501 acgaacgctg tgatgaactg gctcgtgctg cggcaatgaa tcccacactg gaagatacag
     1561 gctatcaagt cgaagtttaa gcctgtggtt tacgacattg ccgggtggca ccaaccgcct
     1621 ggcgaattcg cggtttattc ttgctctgtt tcatcggatt tagcgttaaa ggaatagtcc
     1681 gtttccgggc aacaataagt tgtaagcaac caagcgcagg aatatgcgca ttcaatagtt
     1741 ttcctccgtg tttgttccac gggagaacgt ggaaacggct ggcgtgtagc acttcaaaat
     1801 tcaacaaaga gagccagtcc agctgccgca tcagagtaaa catccggctg ttatagggcg
     1861 atgttttgcg caataccggc acaagtttgc gtaatcccat taaactgatg ggattgaagc
     1921 cactaatcac cagccaacca tcatcaatca gtacccgatc ggcttcacgc aataaacgat
     1981 gcggatcagt gcaccacggc aatgtatgtg ccagtagaca aacatcaacg gatttatcgg
     2041 caaaaggaag atgaagtggg tccgcctgca cctggacggg cattccttgc gcagaaacat
     2101 tcacttgatg agaaaccgcg cacgcttcgc aattgatttc tgcgcttaaa ttgccaatct
     2161 taagcagatg aaaaccatac attttagtga accacgggtt gagctgacgc tccagcgcct
     2221 tgcgataaag ctctccccag ggcaaatcgc cccagcaatc aggagccacg acagtttgag
     2281 ggactcttgc cggtttcatc acaaccttcc gtttcacact gagaggtaat ctatgaatct
     2341 taacagtatt cccgcctttg atgacaatta catctgggtt ttgaatgatg aagcaggtcg
     2401 ctgtctgatt gtcgatcccg gggacgcaga gccagtatta aacgccattt ccgccaataa
     2461 ctggcaaccg gaggccatat ttctcaccca ccatcaccac gatcacgttg gcggcgtaaa
     2521 agaactggtg gaaaagtttc cgcaaattgt ggtgtatgga ccacaagaga cacaagataa
     2581 gggaacaaca caggtagtca aagatggcga aactgccttc gttttggggc atgaatttag
     2641 tgtaattgcc acaccgggtc acactttagg acatatctgt tacttcagta aaccttatct
     2701 attttgcggc gacacgctgt tttctggtgg gtgtggtcgg ttgtttgaag ggacaccatc
     2761 acaaatgtat caatcactta aaaagttaag tgcgttacct gacgacacat tggtatgttg
     2821 tgctcatgaa tataccttat caaatatgaa gtttgctttg agtattct
//
LOCUS       DAZGJT010000228         2833 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZGJT010000228 DAZGJT010000000
VERSION     DAZGJT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.233"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            151..474
                     /locus_tag="ACHKFK_004833"
     CDS             151..474
                     /locus_tag="ACHKFK_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO9054966.1"
                     /translation="MRLLILTLSLITLTGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HARTARYVSRGIADRACQQAGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            646..1143
                     /gene="ftnA"
                     /locus_tag="ACHKFK_004834"
     CDS             646..1143
                     /gene="ftnA"
                     /locus_tag="ACHKFK_004834"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO9054967.1"
                     /translation="MLKPEMIKKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(1181..1420)
                     /locus_tag="ACHKFK_004835"
     CDS             complement(1181..1420)
                     /locus_tag="ACHKFK_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO9054968.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            1611..2822
                     /gene="tyrP"
                     /locus_tag="ACHKFK_004836"
     CDS             1611..2822
                     /gene="tyrP"
                     /locus_tag="ACHKFK_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO9054969.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQLATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
BASE COUNT          663 a          659 c          683 g          828 t
ORIGIN      
        1 atgttataaa aatgataatc aaaaagcagc ccccccattt ctgacacata cagatggcaa
       61 gaaatagcgc ctgccaggcg tcttttccgg ccattgtcgc agcgctgtaa cgcgtaaaat
      121 actgctttct tttactcttc tggctggacc atgagacttc tgattctgac tctttcctta
      181 ataacgctga caggatgtac ggttactcgc caggcccatg tgagcgaagt tgacgcggca
      241 acaggtattg ttcggttggt ttatgatcag gcttttttgc agcatgcccg taccgctcgt
      301 tatgtcagtc gcgggatcgc cgatcgcgca tgccagcagg caggctatac ccacgctatc
      361 ccctttggtc agccagtagg caactgtagc ctctttgcgg gttccttatg tttgaatacg
      421 gaattcactt tgtcatatca gtgccatcat tccgccttcc ctgtctttct ctaaacgttt
      481 taatcagcga gggggatctt cgctgattaa ataaatagcg gtaatgcgtt ttattcccat
      541 tcgtattttt aataaatgat gtttatattt taccttttgc aaataataaa ataacaaatt
      601 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
      661 atgattaaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
      721 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
      781 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
      841 aacttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
      901 ttattccagg aaacctataa acacgaacaa ttaatcacgc agaaaattaa cgaactggct
      961 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcaatg gtatgtttct
     1021 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     1081 aaaagtggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     1141 taatgctctc cggcggcagg ttttctgccg ccgtttcaaa tcagtgacgg caaatcttac
     1201 tctgatcggt tgaaaaacct tcttctgaag gtataaattt gccttttgct gccagaaacg
     1261 ccaccaactc tcccgcagtc atcccttctg ccgagcaggt gtgaaaacgt gcctgttcac
     1321 caaaacgcgc tttaattgct gcttccagac tggcatgcgt atattgctcg cctgattcaa
     1381 tcatcatatt taacacttcg tgaccgtgaa tagagtccat cgttcctcct caaaaaaagc
     1441 ctagcgtagc gattgccgct tatgaagact ttgcgccagc gcagaactga acgcttttta
     1501 ttgtacattt atatttacaa catatgtaac gtcggtttga cgaatcagcc gttatgcctt
     1561 aacctgcgcc gcagatatca ctcataaaga tcgtcaggac agaagaaagc gtgaaaaaca
     1621 gaactctggg aagtgttttt atcgtggcgg gaaccacaat tggcgctggt atgctggcaa
     1681 tgccgctggc tgcggcaggt gttggtttta gcgtcacgtt aatcttgtta attgggcttt
     1741 gggcgttgat gtgctacacg gcgctattac tgctggaggt gtaccagcat gtcccggcag
     1801 ataccggtct gggcacgctg gcaaaacgct atctgggacg ctacggtcag tggctgacgg
     1861 gcttcagtat gatgttctta atgtatgctc tgactgcggc atacatcagc ggtgccggtg
     1921 aattgttggc ctccagcatc agcgactgga caggtatttc aatgtcggca accgctggcg
     1981 tgctgttgtt cacttttgtt gccggtggcg tggtttgtgt cggaacttcg ctggtcgatt
     2041 tatttaaccg ttttctgttc agcgccaaaa ttatttttct ggtggtgatg ctggtattgc
     2101 tgctaccgca tattcacaaa gtgaatcttt taaccctgcc gttgcaacag ggactggcac
     2161 tgtctgcaat cccggtgatt tttacgtcgt ttggttttca cggtagcgtg ccgagtattg
     2221 tcagctatat ggatggcaac attcgtaagc tacgctgggt gtttataatc ggtagtgcga
     2281 tccccctggt ggcatatatt ttctggcagc tggcgaccct tggcagcatt gattcaacaa
     2341 cctttatggg attgctggct aatcatgccg gattaaacgg gctgttacag gcgttacgcg
     2401 aaatggtggc ctctccgcat gttgagctgg cagtgcattt atttgctgat ttagccctcg
     2461 ccacgtcctt cctcggcgta gcgttaggct tatttgatta tctggcagat ttgtttcagc
     2521 gttcaaatac cgttggtgga cgattgcaaa ctggtgcaat tacctttctg ccgccactgg
     2581 cgtttgcact gttttatcca cgaggatttg tgatggcgct gggttacgcc ggtgtggcac
     2641 tggcggtact ggcattgatt atcccttcgc tgttgacctg gcaaagcagg aagcacaatc
     2701 ctcaggcggg ttaccgggtg aaaggtggtc gtccggcgct ggtggtggtg tttctctgtg
     2761 gtattgctgt gattggcgtg caatttttga ttgcggcagg cttgttacca gaagtggggt
     2821 gattagatag cct
//
LOCUS       DAZGJT010000229         2826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZGJT010000229 DAZGJT010000000
VERSION     DAZGJT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.234"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..2786)
                     /gene="chiA"
                     /locus_tag="ACHKFK_004837"
     CDS             complement(84..2786)
                     /gene="chiA"
                     /locus_tag="ACHKFK_004837"
                     /EC_number="3.2.1.14"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chitinase/lysozyme"
                     /protein_id="HFO9054970.1"
                     /translation="MKLNIFTKSMIGMGLVCSALPALAMEAWNNQQGGNKYQVIFDGK
                     IYENAWWVSSTNCPGKAKANDATSPWRLKRTATAAEISQFGNTLSCEKSGSSSSSTSN
                     TPASNTPANGGSATPAQGTVPSNSSVVAWNKQQGGQTWYVVFNGAIYKNAWWVASSNC
                     PGDAKGNDASNPWRYVRAATATEISETSNPQSCTSAPQPAPDVKPAPDVKPAPDVQPA
                     PADKSNDNYAVVAWKGQEGSSTWYVIYNGSIYKNAWWVGAANCPGDAKENDASNPWRY
                     VRAATATEITQYGNPGSCSVKPDNNGGAVTPVDPTPETPETPVTPTPDNNEPSTPADS
                     SNDYSLQAWSGQEGSEIYHVIFNGNVYQNAWWVGSEDCPRGTSVENSNNPWRLVRTAT
                     AAEMSQYGNPTTCEIDNGGVIIADGFQASKAYSANSIVDYNDAHYKTSVDQDAWGFVP
                     GGDNPWKKYEPAKAWSASTVYVKGDRVVVDGQAYEALFWTQSDNPALVANQNATGSNS
                     RPWKPLGKTQSYSNEELNNAPQFNPETLYASDTLIRFNGENYISQSKVQKVSPSDSNP
                     WRVFVDWTGTKERVGTPKKAWPKHVYAPYVDFTLNTIPDLAALAKNHNVNHFTLAFVV
                     SKDANTCLPTWGTAYGMQNYAQYSKIKALREAGGDVMLSIGGANNAPLAASCKNVDDL
                     MQHYYDIVDNLNLRVLDFDIEGTWVADQASIERRNLAVKKVQDKWKSEGKDIAIWYTL
                     PILPTGLTPEGMNVLSDAKAKGVELAGVNVMTMDYGNAICQSANTEGQNIHGKCATSA
                     IANLHAQLKGLYGNKSDAEIDAMMGTTPMVGVNDVQGEVFYLSDARLVMQDAQKRNLG
                     MVGIWSIARDLPGGTNLSPEFHGLTKEQAPKYAFSEIFAPFTKQ"
BASE COUNT          681 a          738 c          659 g          748 t
ORIGIN      
        1 gaaatatgaa taaaaataat gggctaccaa aagtagccca ttgacaaaaa atgcggcgat
       61 accggaaggt atcgccaaca catttattgc ttagtaaacg gcgcgaagat ttcgctaaat
      121 gcgtacttcg gagcctgttc tttagtcaga ccgtggaatt ccggagacag gttagtgccg
      181 cccggcaggt cgcgcgcgat tgaccagata ccaaccatac cgagattacg cttctgcgca
      241 tcctgcatga ccagacgagc atcagagaga tagaacacct caccctgaac gtcgttcacg
      301 ccaaccatcg gcgtggtacc catcatggcg tcaatttctg catcactctt attgccatag
      361 aggcctttca gctgcgcatg caggttggca atcgcagacg ttgcacactt accgtggatg
      421 ttctggcctt cggtgtttgc agactgacaa atagcgttac cgtagtccat tgtcatcacg
      481 ttcacacccg ccagctcaac acctttcgct ttggcatcgc tcaggacatt catcccttcc
      541 ggcgtcaggc ctgtcggcag aattggcaag gtataccaga tagcaatatc tttgccttct
      601 gacttccatt tatcctgcac tttcttcaca gcaaggttac gacgttcaat agatgcctga
      661 tccgcaaccc aggtgccttc gatatcgaag tccaggactc tgaggttcag gttatcaacg
      721 atgtcataat aatgctgcat tagatcgtca acgttcttac aggaagctgc cagcggggca
      781 ttattagcac caccgataga cagcatcaca tcaccgccag cctcacgcag agctttgatt
      841 ttgctgtact gagcgtaatt ctgcataccg taagcggtac cccatgtcgg tagacaggtg
      901 ttcgcatctt tactcaccac aaacgccagc gtgaagtggt tgacgttatg attcttagcc
      961 agcgcagcca aatccgggat cgtattcagc gtaaagtcga catacggtgc ataaacgtgt
     1021 ttcggccacg ctttcttcgg cgtacctacg cgctctttgg ttccggtcca gtcaacaaaa
     1081 acacgccacg ggttgctgtc agaaggagaa actttctgca ctttactctg agaaatgtag
     1141 ttctcaccgt taaagcgaat cagcgtatca ctggcataaa gcgtttctgg attaaactgc
     1201 ggcgcattat tcagctcttc gttgctatag ctctgagtct tacctaacgg cttccacggg
     1261 cgactattgc taccggtggc gttttggttc gcaaccagag cagggttgtc actttgcgtc
     1321 cagaacagcg cttcataagc ctgcccatca acaacaacgc gatcaccttt cacgtacacg
     1381 gtggaagcgg accatgcttt cgccggttcg tatttcttcc acgggttatc gccgccgggg
     1441 acaaatcccc atgcgtcttg atcgacagag gttttataat gtgcatcgtt ataatctacg
     1501 atgctgttcg cgctgtacgc tttgctggcc tggaaaccat ccgcaataat gacgccgcca
     1561 ttatcaattt cacaggtagt cgggttaccg tactgactca tttccgcagc ggtagctgta
     1621 cgcacgaggc gccatgggtt attggagttt tctacactgg tgccacgcgg gcaatcttca
     1681 gaaccaaccc accaggcatt ctgataaaca ttaccattga agataacatg gtaaatttcg
     1741 ctgccttcct ggccactcca cgcctgcagt gagtaatcgt tactgctatc cgctggtgtt
     1801 gatggctcgt tgttatccgg agttggagta actggagttt ccggtgtttc cggagttgga
     1861 tcaaccggag tcacagcacc gccattatta tccggcttaa cggaacagga gcctgggtta
     1921 ccatactgag tgatttctgt tgccgttgcc gcgcgaacat aacgccatgg gttgctggca
     1981 tcgttttctt tcgcatcgcc tgggcaattt gccgcgccta cccaccaggc gttcttataa
     2041 atgctgccgt tgtagataac gtaccatgta gaagaacctt cctgaccttt ccaggctacg
     2101 acagcatagt tgtcgtttga cttatcagct ggggctggct gaacatcagg agccggttta
     2161 acgtccggtg ccggtttcac atccggtgca ggctgtggtg ctgaagtaca agactgtgga
     2221 ttactggttt ctgagatttc cgttgccgta gcggcacgaa cataacgcca tgggttgctg
     2281 gcatcattgc ctttcgcatc acccgggcag ttagaagagg ctacccacca ggcattttta
     2341 tatatcgcac cattaaagac gacataccag gtttgaccgc cctgctgttt attccaggca
     2401 actacagatg aattagacgg aacagtgccc tgtgctggtg tagccgaacc gccattagca
     2461 ggcgtattag atgctggcgt atttgaagtt gaagaagatg agcttccgct cttttcgcag
     2521 gaaagtgtat tgccaaactg actaatttca gcagctgttg cggtacgctt taaacgccac
     2581 gggctagttg catcatttgc tttcgctttt cccgggcaat ttgtagaaga aacccaccag
     2641 gcattttcat aaattttgcc atcgaaaata acctgatatt tattaccacc ttgttggtta
     2701 ttccatgctt ccattgccaa tgctggcaga gcggaacaca ccagccccat accaatcata
     2761 gatttagtaa atatatttaa tttcattata atcccttgta acgtaaaaac tgcaaaacaa
     2821 aagtcc
//
LOCUS       DAZGJT010000230         2813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZGJT010000230 DAZGJT010000000
VERSION     DAZGJT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.235"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..1106
                     /locus_tag="ACHKFK_004838"
     CDS             99..1106
                     /locus_tag="ACHKFK_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054971.1"
                     /translation="MDFEFTGEETPEQLEKMLEGLGDVDIDGHEQDGVTEVATENHAD
                     EAAQTQTGDNTAAPTPDASAEQTQDEKEPEAKGVLTRDGKHVIPYEVLEAERSGKQRA
                     EQEAALLRGQIAEEKRLVELLTSQIHQAGMKPAPLPENEKISDEKIARIREMYPEIGD
                     AVASLIRKNNYLQSRVQQSAQQAEGHGGEDLSPVLDAMNAVPVLKTWQNSDPDRFSVA
                     VSIDGKLQNDPAWKDKPLTERFAEVARRTQLAFGEASEPPSDDKADNTDIRKTAEEKV
                     KAAEREMAVPASPSELGNTASVGTGDKFEQLLSASHTEIEAAMRGMTDAEIDAILEKL
                     G"
     gene            1129..2343
                     /locus_tag="ACHKFK_004839"
     CDS             1129..2343
                     /locus_tag="ACHKFK_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N4-gp56 family major capsid protein"
                     /protein_id="HFO9054972.1"
                     /translation="MTTVTSAQANKLYQVALFTAANRNRSMVNILTEQQDAPKAVSPD
                     KKSTKQTSAGAPVVRITDLNKQAGDEVTFSIMHKLSKRPTMGDERVEGRGEDLSHADF
                     SLKINQGRHLVDAGGRMSQQRTKFNLASSARTLLGTYFNDLQDQCAIVHLAGARGDFV
                     ADDTILPTADHPEFKKIMINDVLPPTHDRHFFGGDATSFEGIDQSDLFTLALVDNLSL
                     FIDEMAHPLQPVRLSGDELYGEDPYYVLYVTPRQWNDWYTSTSGKDWNQMMTRAVNRS
                     KGFNHPLFKGECAMWRNILVRKYAGMPIRFFTNSKVLVSKNDMAATTEEKQAKTNIDR
                     AMLLGAQALANAYGQKGGGHFNMVEKKTDMDNRTEIAISWINGLKKIRFPEKSGKMQD
                     HGVIAVDTAVKL"
     gene            2425..2787
                     /locus_tag="ACHKFK_004840"
     CDS             2425..2787
                     /locus_tag="ACHKFK_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054973.1"
                     /translation="MSERVYTGTHGNESVAEGVFTVSAAEQETVIHLLSLPAGIRINS
                     LQLVSTGGLGTATVSVKSGEHVLIDNSEAVSAKFAKYVPVEPYTTQSDGELVTVTIKT
                     AAATGTLNVLLRYTVVGY"
BASE COUNT          724 a          705 c          787 g          597 t
ORIGIN      
        1 cgttccggga gcggtgcgct tattcgcggg ggcagcgata agccttattt actcaaccat
       61 tcggatctgt ccgataaaca gaccatgcgg agttatttat ggattttgaa tttacgggtg
      121 aagaaacccc ggaacaactg gaaaaaatgc tggaagggct tggggatgtg gatattgacg
      181 gtcacgaaca ggacggcgtg acagaggtcg ccacagaaaa tcatgcggat gaggcagcac
      241 agacacagac gggcgataac actgcggcac cgacgccgga tgccagtgct gagcagacgc
      301 aggacgagaa ggagccggag gcgaagggtg tgctcacccg cgatggtaaa cacgtcattc
      361 cctatgaagt ccttgaggct gaacgttccg gtaagcagcg ggccgaacag gaagccgcac
      421 ttcttcgggg acagatagcc gaagaaaaac gcctggttga actgctgacg tctcagatcc
      481 accaggccgg tatgaaaccc gcaccgttac cggaaaacga aaaaatctct gatgagaaga
      541 ttgcccgtat cagggagatg tatccggaaa ttggtgacgc ggtggcttcg ctcatccgta
      601 aaaataacta tctccagtcc cgtgttcagc aatcagcaca gcaggcagaa ggtcatggtg
      661 gtgaggattt atcaccggtt cttgatgcga tgaatgccgt gccggtgctg aaaacgtggc
      721 agaactccga tccggatcgc ttctcggttg ccgtatccat cgacgggaag ctccagaatg
      781 accccgcatg gaaagacaaa ccgctcactg aacgtttcgc cgaagtggcc cgccgtacgc
      841 agctcgcttt cggtgaagcc agtgagccgc cttctgatga caaggcagac aacacggata
      901 tccggaaaac ggcggaagaa aaagtgaaag ccgctgaacg ggagatggca gtgcctgctt
      961 ccccgtcaga gcttggcaac acggcttccg tcggaaccgg tgataaattt gaacagttac
     1021 tgagtgcttc ccataccgaa attgaagcag ccatgcgagg catgacggac gctgaaattg
     1081 acgcgattct ggaaaaactc gggtaaccac tgaaggagta ctgacgtaat gaccacagta
     1141 acatcagccc aggcgaataa gctgtatcag gtggcgcttt tcacggctgc caaccgcaac
     1201 cgctcaatgg tgaatatact cactgaacag caggatgcac caaaagcagt ttcgcctgac
     1261 aagaaaagca cgaagcagac cagtgcaggt gcaccggttg tgcgaatcac ggacctcaac
     1321 aaacaggccg gtgatgaagt gacgttcagc atcatgcaca aactctctaa acgtccgacg
     1381 atgggggatg agcgcgttga aggccgtggt gaagacctca gccatgctga tttttccctg
     1441 aaaattaatc agggccgtca cctggtggat gcgggcggac gtatgagcca gcagcgtacg
     1501 aagttcaacc tggcatcctc tgccagaacg ctgctgggta cgtactttaa cgacctgcag
     1561 gaccagtgtg cgatagtgca tcttgccggt gcccgcggtg attttgttgc tgatgacacc
     1621 attctgccaa cggcagatca ccctgaattc aaaaaaatta tgatcaacga cgtactgccg
     1681 ccgacgcatg atcgtcattt tttcggtggg gatgcgacga gttttgaagg tattgaccag
     1741 tctgacctgt tcactcttgc gctggtcgat aatctctcac tgtttattga cgagatggct
     1801 catccgttac aaccggtacg cctgtccggt gatgagctgt atggcgaaga tccgtattac
     1861 gtcctctacg tcacgccgcg tcagtggaat gactggtaca cctccacgtc cggcaaggac
     1921 tggaaccaga tgatgacgcg tgccgtaaac cgctcaaaag gttttaacca tccgctgttc
     1981 aaaggcgaat gtgcgatgtg gcgcaatatt ctggtccgta aatatgcggg gatgccgatc
     2041 cgtttcttta cgaactcaaa agttctggta tcgaaaaacg atatggcagc aaccacagag
     2101 gagaagcagg ccaagaccaa tatcgaccgt gccatgctgc tgggcgctca ggcgctggcg
     2161 aatgcgtacg gtcagaaggg cggcggtcac ttcaacatgg ttgagaagaa aacggatatg
     2221 gataaccgta ctgagatagc aatcagctgg atcaacggcc tgaaaaaaat ccgtttcccg
     2281 gagaagagcg gcaagatgca ggatcacggc gtgattgccg ttgataccgc agtgaagctc
     2341 tgatttttcc tttccccatg ccgggttatc gcccggcttt ttcaggagtc attaattatg
     2401 gcaaagacta tccttgctcc gtaactgagt gagcgggtct atacgggtac gcacggtaat
     2461 gagtcggtgg cagaaggtgt gtttacagtg agtgctgccg aacaggaaac cgttattcat
     2521 cttctctcac tgccagctgg catccgtatc aactcactcc aactggtttc aacgggcggt
     2581 ctgggtaccg caaccgtcag cgtcaagtcc ggtgagcatg ttctcatcga taacagcgaa
     2641 gctgtttctg caaaatttgc taaatatgtg ccagtggagc cgtacaccac acagagtgac
     2701 ggggagctgg ttacggtcac cattaagaca gctgctgcga ccggtaccct gaatgttctg
     2761 ctgcgttata ccgtggtggg atactgatat aaaccttccg gcccgcgtca tgc
//
LOCUS       DAZGJT010000231         2732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZGJT010000231 DAZGJT010000000
VERSION     DAZGJT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.236"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..324
                     /locus_tag="ACHKFK_004841"
     CDS             <1..324
                     /locus_tag="ACHKFK_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9054974.1"
                     /translation="SARTDIRANYWSLLSGPSWRFNEYLSVYAMAGMGVAKVTTDLKI
                     NDNLNHGAGSFSESNSTKKTSIAWSVGAQFNFNESVTMDVTYESSGSGDWKTDGLFAG
                     IGLKF"
     gene            556..696
                     /locus_tag="ACHKFK_004842"
     CDS             556..696
                     /locus_tag="ACHKFK_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9054975.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            753..1154
                     /locus_tag="ACHKFK_004843"
     CDS             753..1154
                     /locus_tag="ACHKFK_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054976.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSADGIPYVLVLLGVCTGSNSVERYLPEVKTLTRLAGGRWAEFHT
                     ARRGFIRLGKRLGFERMPDDEDGFMVFRIAV"
     gene            1246..1902
                     /locus_tag="ACHKFK_004844"
     CDS             1246..1902
                     /locus_tag="ACHKFK_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054977.1"
                     /translation="MGGKKGGGDTKVKPTAAQIAQEEVAWKGWQDYQKILRPAEDNFM
                     EKVDNLNSEQQYDNIAGTTNLGYQKQFGEARRELAGNLAQSGVDPSSGRFNAVMDANQ
                     SDQVIGQIDMTTRGQVSQADKYIAGLQDVAALGSGQKADALQSFNSLADSSLAKAKSD
                     AQAAFTKQQGRASLVGAGLGAVGAYAMHKAGGNGGGGGAKTPGTGANAIQHQAQNWRL
                     "
     gene            1905..2351
                     /locus_tag="ACHKFK_004845"
     CDS             1905..2351
                     /locus_tag="ACHKFK_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054978.1"
                     /translation="MEYGKYETLARAGYSGAARPQGDWQTSAALTRQQYDDWRTRYLP
                     RVARLADLGENNSLMNAQLARVGGLATSSLRTAQMAQDNQMARYGVNRPDNPNSNTLG
                     LRNALAIAGAKNGIREAEQDRQMNILTGASAPARQQLSVGGQLVSA"
     gene            2361..2612
                     /locus_tag="ACHKFK_004846"
     CDS             2361..2612
                     /locus_tag="ACHKFK_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HFO9054979.1"
                     /translation="MGYGLLDIANQSRREALQGISDADRRREEIEAANKQMAAQQKAQ
                     NKQNIGTGIGTGAAMGAAVGGLPGAAIGAVIGGLAGSLF"
     gene            2623..>2732
                     /locus_tag="ACHKFK_004847"
     CDS             2623..>2732
                     /locus_tag="ACHKFK_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054980.1"
                     /translation="MSGFAQGLLAGFSTVDQAMTRRKELGLREAQLAQQQ"
BASE COUNT          691 a          602 c          811 g          628 t
ORIGIN      
        1 tcagcgagaa ctgatatcag ggctaattac tggagtctac tatccggtcc ttcgtggcgg
       61 tttaacgaat acctcagtgt gtatgcaatg gctggcatgg gggttgcaaa agtcaccact
      121 gacttgaaaa ttaacgacaa tcttaatcat ggtgccggta gtttttctga aagtaacagc
      181 accaaaaaaa cgtctattgc atggtctgtc ggcgcgcaat ttaacttcaa tgaaagtgtt
      241 actatggacg tcacttatga aagctcaggc tctggcgact ggaaaactga tggtttattt
      301 gcaggcattg gcctgaaatt ctgagtaata cccgacaaca tgtcataacc cgctgtgatg
      361 gcgggttttt tgttgcccgt gcagggcaaa aatcgtagat tatgcgcggg tgcctttcgg
      421 ctgatggctg gaaggtgaac ctgaaggcct gatgtggaaa ggccccgagt caactttaac
      481 gttaacccga ggccctgaca cttctttcct tgacaagtag aaggttagcg cctctccact
      541 caggaggcaa gcgctatgtc gcaaaaatcg cttatcaccg tcacaatttg catgacggta
      601 atcttcacca tctggatgtt acacggttcg ctgtgtgagt tccggctgaa tttgtgggga
      661 gcggagtttg cggcgttctt acagtgtaag cagtaggaaa accgcgacgg ggacgaaagt
      721 ccccgtcaac tggttgctga ggttcagccg atatggcacc cgtttcaggt gagagaatgg
      781 acgacaaaat tctccggtat atgcagcgtg ttgtgagaaa ttcccgcaac cctgaattta
      841 tgaatgaagt taaagacgcc tgccttaaaa agcaggcgtt ttgttttgag gcacctgatg
      901 gctttctggt gctgcgttct gtgctcagtg ctgatggtat cccttatgtt ctggtgttgc
      961 tgggcgtgtg tacggggagt aacagcgttg agcgttatct gccggaggtg aagacattaa
     1021 cccgtctggc tggcggacgc tgggctgaat ttcatacggc aaggcgggga tttatccggc
     1081 tgggaaaacg tctgggcttt gagcgaatgc cggatgatga ggatggcttc atggtgttca
     1141 ggatagcggt ctgactgcca ctgtattcat tatcgtgtgt aaaccaattg caattcacat
     1201 tctgaccctg ccccggcagg gttttttgtt atctgaggag ccataatggg cggaaaaaaa
     1261 ggcggtggtg ataccaaagt aaaaccaaca gcagcgcaaa tagcacagga agaagtggcc
     1321 tggaaagggt ggcaggatta tcaaaaaatc ctccgcccgg ctgaagataa cttcatggaa
     1381 aaggtcgata accttaacag tgagcagcag tacgacaata ttgctggcac cacaaatctg
     1441 gggtatcaga aacagtttgg tgaagcgcga agggagctgg cgggtaatct tgctcagtcc
     1501 ggcgttgacc catccagtgg tcgttttaat gcagtaatgg atgcaaatca aagcgaccag
     1561 gtgatcgggc agattgacat gaccacacgg gggcaggtat cgcaggcaga taaatatatt
     1621 gccgggctac aggatgttgc ggctctcggt tccggtcaga aggcagatgc gttacagagt
     1681 tttaactcct tggctgacag cagtctggca aaagccaaat cggatgcaca ggcggcgttt
     1741 acgaaacaac aggggcgagc ttctcttgtt ggcgctggtc tgggagctgt aggtgcgtac
     1801 gcgatgcata aggctggcgg taacggaggt ggtggcggcg ctaaaacgcc aggcaccggc
     1861 gctaatgcta ttcagcatca ggctcagaac tggagactgt aattatggag tatggcaaat
     1921 acgaaactct cgcaagggct ggttattcag gcgcagcccg cccacagggt gactggcaga
     1981 catccgccgc gctgacacgc cagcaatacg acgactggcg aaccagatat ttgccccgcg
     2041 tggcaaggct ggctgacctt ggtgagaaca acagcctgat gaatgcacag cttgcccggg
     2101 taggtggcct tgccacttcc agtctccgta cagcgcagat ggcacaggat aaccagatgg
     2161 cgagatacgg ggtaaaccgt ccggataatc ccaacagtaa cacgctgggt ttacgtaatg
     2221 cactggcaat tgctggcgcg aaaaatggta tacgggaagc agagcaggat cgccagatga
     2281 acatactgac gggggcttct gcacctgcaa gacagcaact gagtgttggc ggacaactgg
     2341 tgtcagcgta aggaggaaat atgggatacg gtttactgga tattgcgaat cagtcgcggc
     2401 gtgaggcatt acagggaata agtgatgccg acagacgacg tgaagaaatt gaggccgcga
     2461 acaaacagat ggcggcgcaa cagaaagcgc agaacaaaca gaatatcggc acgggaattg
     2521 gtacgggtgc agctatgggc gctgctgttg gtggtctccc cggtgccgct attggtgcag
     2581 tcatcggtgg tcttgctggt tcgttgtttt aaggagtggt gaatgagcgg atttgcacag
     2641 gggttacttg ccggattcag caccgttgac caggccatga cccgtcgtaa ggaacttggt
     2701 ttgcgagaag cacagcttgc tcaacaacag aa
//
LOCUS       DAZGJT010000232         2703 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZGJT010000232 DAZGJT010000000
VERSION     DAZGJT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.237"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1122)
                     /locus_tag="ACHKFK_004848"
     CDS             complement(<1..1122)
                     /locus_tag="ACHKFK_004848"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MalY/PatB family protein"
                     /protein_id="HFO9054981.1"
                     /translation="MDVFNTPVSRKGTYCTQWDFCEDRFGVKDVLPFSISDMDLPIPD
                     AITRALKKRLEHPILGYSRWQHGEYLNAIVNWYYQQYQTDIKPEWITYSPSVMYSIAK
                     AIELLTSHGDNIIVFTPVYNAFFDVIKHSERNILTASLIIKNEGGYAINWQDFDLKIK
                     SAKMVLLCNPHNPTGTVWSEEELHKIAASCTRHNVWLCSDEIHSDFVFNRSFTSALKI
                     KKEKVVVFNSISKTFNVPALTGSYMISTDDNFNHKFRTISRYRDFVNSPSVLNIIATI
                     IAYNECEGWLKSLKAHIASNIQFTQQYLNENIPELIVRPADGCYFSWIDCSAIGYPFD
                     EFYSRLIHEGKVGIMAGHVYGTEGEGYLRLNLACGREKLY"
     gene            complement(1122..2702)
                     /locus_tag="ACHKFK_004849"
     CDS             complement(1122..2702)
                     /locus_tag="ACHKFK_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HFO9054982.1"
                     /translation="MKQKKAWSFFQSLGKAFMYPIALLSVCGMMLGLGSGLASDDMAK
                     LIPFLAIPIIKTILDFIVSLGLFAFVNLPVLFAIAIPLGLLKDKEDKAYGTFSGLIGF
                     MAMHLGTNFYLKQHDLLVVADQMSTHGQTIILGIQSYNTSVLGGIVAGLLVASMYKKI
                     VNLRIPESLGFYSGPRLVPIITLIVMSGFGLIIPFIWPPFFNLFMLIGHWISTSGPVG
                     YFFYAVAERVTIPFGLNHLVTSVFRFTPIGGSAVIGGEEYYGTLNMFMAYVKENAVIP
                     LDLAGKMEQGKLMIQYGLAGAALAMYRTAHAQNRKAIKALLISGVLTVIIGGVSEPIE
                     FLFLFVSPLLFVFHAFMNGFANMVLPYMGVKMGFTGDLIQFISFGVLRGTRTGWPIAV
                     CVEVAYFFIYYFVFRWTILKFNLMTVGREESNPVTLNVHEDTAIADIPTPDKSELQAA
                     EQMVKALGGKENIKSLDNCVTRLRLTIADMGLIDEVAIKRAGGIAVVKLDQNTLQVII
                     GTKVIALRRDMDNYMGIY"
BASE COUNT          842 a          620 c          498 g          743 t
ORIGIN      
        1 atataatttt tctcgaccgc aggccaggtt cagacgaagg tagccctccc cctccgttcc
       61 atagacatgc cccgccataa tgcccacttt gccttcatga attaatcgac tgtagaattc
      121 atcaaaagga taaccaatag cagaacaatc tatccaggaa aaatagcaac catcagctgg
      181 ccttacgatc aattctggaa tattttcatt cagatactgc tgggtgaatt gaatgttaga
      241 ggcaatatgt gcctttaatg actttaacca tccctcacat tcgttatagg caataatagt
      301 tgcaattata ttcagtactg atggggagtt tacaaaatcg cgatacctcg atatcgttct
      361 gaacttatga ttaaaattat cgtcagttga aatcatatac gatcctgtta gtgcaggaac
      421 attaaatgtt ttcgatatcg agttaaatac tactactttt tcttttttta ttttaagcgc
      481 ggatgtaaag ctacgattaa aaacaaaatc actatggatt tcatctgaac ataaccagac
      541 attatgcctt gtacagcttg cagcgatttt atgcagttct tcttccgacc agactgttcc
      601 tgtcgggtta tgaggattac ataataacac catctttgcg cttttaatct taagatcaaa
      661 atcttgccag ttgatagcgt agcccccctc attttttata atcaaagagg ctgtcagaat
      721 atttcgctcg ctatgtttaa ttacatcaaa aaatgcatta tagacaggtg taaaaacgat
      781 aatattatca ccatgcgatg tcaataactc tattgctttt gcaatagagt acatcacact
      841 ggggctatag gttatccatt caggtttaat atctgtttga tattgttgat aataccagtt
      901 tactatcgca ttcagatatt caccatgttg ccaacgactg taccctaaaa tcgggtgttc
      961 caggcgtttt tttaatgctc tggtgatagc gtctggtatc ggaagatcca tatctgaaat
     1021 agagaatggt agtacatctt ttacaccaaa tctgtcctcg caaaagtccc attgggtaca
     1081 atatgttcct ttgcgggaaa caggagtatt aaaaacatcc atcagtatat ccccatatag
     1141 ttatccatat cccgacgcag ggcgatgact ttagtgccga taatgacttg tagggtattt
     1201 tgatcaagtt taacaaccgc aatcccgcca gctcttttta ttgcaacttc gtcaatcaat
     1261 cccatatctg cgattgttaa acgtaaacga gttacgcaat tatccagtga cttaatattc
     1321 tctttaccac caagtgcctt aaccatctgc tccgccgctt gcagctctga tttatcagga
     1381 gttgggatat ccgctatagc cgtatcttcg tgaacgttca gcgtgacagg attggactct
     1441 tcacggccta cggtcatcag gttaaattta agaatggtcc aacggaacac aaagtaataa
     1501 atgaagaaat aggccacttc gacacacacc gcgatcggcc aacctgttct tgtgccacgc
     1561 aatacgccaa agctaataaa ttgaatcaga tcgccagtaa atcccatctt cacgcccata
     1621 tatggcagaa ccatgttagc gaatccattc ataaaggcat ggaagacaaa cagcagtgga
     1681 ctgacaaata agaacagaaa ctcaatcggt tcgctgacgc cgccaataat caccgtaagc
     1741 accccggaaa taagcaatgc tttgatagcc tttctgtttt gagcatgagc agtgcgatac
     1801 atcgccagcg cggcaccagc cagaccatac tgaatcatca gtttgccctg ctccattttc
     1861 cccgccaaat ccagcggaat gaccgcattc tctttaacgt atgccataaa catgttcagg
     1921 gtgccgtaat attcttcgcc gccaatcaca gccgaaccgc cgattggcgt aaagcggaaa
     1981 actgacgtca ccagatggtt taagccaaaa ggaatcgtca cacgttcggc aactgcatag
     2041 aagaaataac caacaggacc ggaagttgaa atccagtgtc caatgagcat gaaaagattg
     2101 aaaaacggcg gccagataaa aggaatgatc agaccaaatc cactcatcac aatcagtgta
     2161 atgataggca ccagacgtgg gccgctataa aaacctaatg attctggaat gcgtaaatta
     2221 acgatctttt tatacatgct ggcgactaat aacccagcaa caattccccc caacacgctg
     2281 gtattgtagg actggatccc cagaatgatg gtttgcccat gtgtcgacat ttggtcagca
     2341 acgaccaata agtcgtgctg tttaagataa aagttcgttc ccagatgcat cgccataaaa
     2401 ccaattaagc cagaaaaagt accataggct ttatcctctt tatcttttaa taatcctaag
     2461 ggaatcgcta tcgcaaacaa tacaggtaaa ttaacaaagg caaacaaacc aagactaaca
     2521 atgaaatcaa gtatggtttt aattattgga atagccagaa atggaattaa ctttgccata
     2581 tcatcactgg ctaaaccact tcccagccct agcatcatgc cacatacact tagcagagca
     2641 atgggataca taaatgcctt ccccaggctc tgaaaaaaac tccaggcttt cttttgtttc
     2701 atg
//
LOCUS       DAZGJT010000233         2590 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZGJT010000233 DAZGJT010000000
VERSION     DAZGJT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.238"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1804)
                     /locus_tag="ACHKFK_004850"
     CDS             complement(<1..1804)
                     /locus_tag="ACHKFK_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HFO9054983.1"
                     /translation="MSFLDAKSYDTLFGLPELYEDALPRFSLSCEDIAFARTHRRSHN
                     RLGFAIQLALVRDLGRTLRAGEIPPEAVITVVAEQLDIDPAVFTLYAQREETRREHAR
                     EIVSALELRSVRSSDYRMLIIAATREAATTEKGLPITRAVINKLKDQKLLAPAPAQLI
                     RLAMAGRAAARRLSYRELIRDLKPSSISALEQLLTERVGEKSILGWIAEAPEGVKLKN
                     LKGLIARLEVLRTAAISDEQRKRIHVNRYGIIARDSRVLHAREIQRMASERRHATLVA
                     FVIEKQAAITDLAIDMFCKLIGSTRRRAEISQKERRLKAAEVLEVVAQDHIKLGRALL
                     AARDGNTDIESVITASLGWEGLIASMEAAASVASSHHCSELDELIGRQKSLRKLGRLI
                     FSAFSFRAFRPDDSVLLSIGYLQAFYHGRKLPKQIPLDFMSHKWRRRVIHGENTDLQA
                     WEVAVFVHLRERLKTGDIWVDGSRAWRSFEDYLLPHHVFGQMRAESRLGLAIPDNFAE
                     WRAERTTTLNAKLKALAKAASENAISDAELSSRGLSVSPIREEESDKIKALSRKLYML
                     VPRIRITSLLAEVHSWTSFLDCFTHYRTGEQAEDE"
     gene            complement(1785..2408)
                     /locus_tag="ACHKFK_004851"
     CDS             complement(1785..2408)
                     /locus_tag="ACHKFK_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9054984.1"
                     /translation="MLIGYMRVSSNDERQSVSLQRDALIAAGVDPRHLHQDHASGARD
                     NRPGLKSCLAELREGDVLIVWKLDRLGRSLSHLLRIIEDLKIRGIAFRSLTESIDTTT
                     PHGSFLFNLFGTLAEYERTLITERVNAGLAAALRRGRKGGRPPAIDAEKVDQILAALE
                     GGASKASVCRAFSVPRSTLTDTLRRIDWAGSGGRNLTTDQSNVISRR"
BASE COUNT          665 a          670 c          617 g          638 t
ORIGIN      
        1 cttcatcttc cgcctgttcc ccggtgcgat aatgggtgaa acaatcaaga aaactggtcc
       61 agctatgaac ttctgccagc aaactggtaa ttctgatgcg tggtaccagc atgtagagtt
      121 tacggctgag agccttaatt ttgtcgctct cttcttcgcg aatcggagac acagacaacc
      181 ctctgctgga aagttctgca tcggatatgg cattctccga agcagctttt gccagcgctt
      241 tcagttttgc gttgagtgtg gtggttcgct cagcccgcca ttcagcaaag ttgtccggaa
      301 tggcaagtcc cagccgggat tctgcccgca tctgcccgaa aacatggtga ggaagaaggt
      361 agtcctcaaa actacgccag gcccgactac catcaaccca tatgtcacct gttttcaggc
      421 gttcccgcaa atgaacgaat acggcgactt cccacgcctg cagatcggta ttttctccat
      481 ggatgacacg acgtcgccat ttatgggaca taaaatcgag cggaatttgt ttgggtaatt
      541 ttcggccatg atagaacgcc tgcaaatacc caatcgacaa caaaacgcta tcatccgggc
      601 gaaaagctct gaaagaaaaa gcgctgaaaa tgagtcgtcc aagcttacgt aatgattttt
      661 gtcgtccgat aagttcatcc agttcagaac agtgatggga agatgccacc gaagctgcag
      721 cctccatact ggcaatcaaa ccttcccagc cgagtgatgc tgttattaca gattcaatat
      781 cagtattacc gtcacgtgct gccaggagag cccgtcccag cttaatatga tcctgagcta
      841 cgacttcaag tacttcagcg gctttcagac gacgctcttt ctggcttatc tctgctctgc
      901 gcctcgtgct gccaatcaat ttgcagaaca tatcaattgc cagatctgta attgctgcct
      961 gcttttcaat aacaaaggct accagcgtgg catgacggcg ttcggatgcc attcgttgta
     1021 tttcacgcgc atgtaaaacg cgagaatccc gggcaataat accgtaacgg tttacatgaa
     1081 tccgtttacg ttgctcatcg gagattgccg ctgttcgtaa tacctccagc ctggcaatta
     1141 accccttaag attcttgagt ttcacgcctt ccggcgcttc agctatccag cctaaaatgc
     1201 tcttttctcc gactcgttcg gtaagcaact gctcgagagc gctaatggat gaaggtttca
     1261 gatccctgat gagttcccga taagaaaggc gacgggcagc cgcccggccc gccattgcca
     1321 gacggatgag ttgtgccgga gcaggggcaa gtaacttctg gtccttcagc ttattgataa
     1381 ctgctctggt gatcggcagt cctttttcgg tggttgctgc ttcgcgtgta gccgcgataa
     1441 tgagcattct gtaatcgcta ctccggacgg aacgaagctc caaagccgaa actatttcgc
     1501 gggcatgttc ccggcgggtt tcctcacgtt gtgcgtaaag tgtaaaaaca gcagggtcaa
     1561 tatccagttg ttccgcaaca acggtgataa cagcctccgg gggaatctct cctgcacgca
     1621 gtgtacgacc gagatcacgg acaagtgcaa gctgaatggc aaaacccaga cggttatgag
     1681 atcgacgatg agttctggcg aatgcaatat cctcgcagga gagcgaaaac cggggaagtg
     1741 catcctcata aagttcaggt aatccaaaaa gcgtatcgta cgatttagcg tcgagaaatg
     1801 acattgctct gatccgttgt cagattacgc cccccagatc cggcccagtc aattcgtcgt
     1861 agagtatcgg tgagcgttga gcgtggcaca ctgaatgcgc gacatactga cgctttactg
     1921 gcacctcctt ccagagctgc gaggatttga tcaacctttt cagcatctat cgccggtggc
     1981 cttccgcctt ttctcccccg ccggagagca gcagccagac cagcattaac gcgttctgtg
     2041 ataagtgttc tttcgtactc cgcgagcgtg ccaaaaaggt taaagaggaa cgagccgtgt
     2101 ggcgttgttg tatcaataga ttcagtcaat gatcggaaag caataccacg aattttcagg
     2161 tcttcaataa ttcggagcaa atgtgagagt gaacgaccaa gtcgatccag tttccagact
     2221 atcagtacat caccttcgcg aagttcagcg agacaggact taagtcccgg gcgattatca
     2281 cgagcacctg aagcatgatc ctgatgtaaa tgccggggat ccactccagc agcaatcaac
     2341 gcatcacgct gcaaagaaac tgactgacgt tcatcattgc ttgatacccg catataacca
     2401 atcaacatgt acgacaaacc ttaaaattga agtttccgta cagtttctca ttccgacagg
     2461 gtttgtcacg caaaaaatca cagatttatg gattcaattt tcttcaactg caggacctga
     2521 cggaaaacaa gtgatttccg tcagggagga atcgcttagc gggtgttatc gcagaaattc
     2581 ccacggaacc
//
LOCUS       DAZGJT010000234         2589 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZGJT010000234 DAZGJT010000000
VERSION     DAZGJT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.239"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..249
                     /locus_tag="ACHKFK_004852"
     CDS             <1..249
                     /locus_tag="ACHKFK_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054985.1"
                     /translation="LLRDGEQISDAIHNGVWTYNSAARPLTMSEMVVMFGYVYRQSRP
                     CRLASEMGIHTKTVNTFLYTGMAKNGLYGVSVRRLVGA"
     gene            300..431
                     /locus_tag="ACHKFK_004853"
     CDS             300..431
                     /locus_tag="ACHKFK_004853"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054986.1"
                     /translation="MMNKERCRTIGRKRYTTGAMCFMLFVWLGGVLALVATAAVVMQ"
     gene            612..2237
                     /locus_tag="ACHKFK_004854"
     CDS             612..2237
                     /locus_tag="ACHKFK_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9054987.1"
                     /translation="MPYIDITTMRGMMPRVVTSMLPEHSAVLAEDCHFRFGVITPERQ
                     ISGVEKTFTIKPKTIFHYRDDFWFAWPDVVDVIRSPIAQDPHGRIYYTDGRFPKVTDA
                     TIATKGDGNHPTSSYRLGIPAPTTAPVCTVQQGGDVSDDNPNDDETRFYTETFVSDYG
                     EEGPPGPASLEVTLRTPGTAVQLTLAPVPLQNASIKRRRIYRSASGGGEADFLLVAEL
                     DASVLSYTDKIPAKNLGPSLATWDYLPPPENMTGLCLMANGIAAGFAGNEVMFSEAYL
                     PYAWPEVNRHTTAEDIVAICPLGTSLVVATKGEPYLFSGVSPSTISGSRIPSMQACLS
                     RRSMVAMEGFVLYAGTNGLVSVDANGNAALATEQIISPEQWQSQFNPASIVAYPWRGE
                     YIACYTKPDGKQDVFVFNPAGMDIRYLSTPFDCACVDLVNDVMRVVSGQNMSAIAGGS
                     LPSTIRWHSKVFSLPERTSFSCLRVKSPTPERVGITVLADDVPVIHLAPGSFSGSVVR
                     LPAATGQNWQILVSGFGQVERITLSTSMSELPV"
     gene            2234..>2589
                     /locus_tag="ACHKFK_004855"
     CDS             2234..>2589
                     /locus_tag="ACHKFK_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9054988.1"
                     /translation="MTKKPWRAGKDLSAVVENMEIGTGQRGDGRHAFVTREELVGLKL
                     ARRRTSGGAAYALNPGIEMDSSVMVVDFPSKPQNFKATGGFGSVLLEWDMPNYRGHSL
                     TEIWRGTEDDLADAVLV"
BASE COUNT          595 a          587 c          746 g          661 t
ORIGIN      
        1 ttgctccgtg atggtgagca gataagtgat gccatacaca acggcgtctg gacgtataac
       61 agtgctgccc gcccgctgac gatgtcagag atggtggtga tgttcggtta tgtctaccgc
      121 cagtcgagac cctgtcgcct tgccagtgaa atgggtatcc acacgaagac ggtgaatacc
      181 tttctgtata cgggaatggc gaaaaacggg ctgtatggtg tgagcgtcag acggcttgtt
      241 ggtgcctgat ggcggtcctg ccggatgcgc agaatcttct gatagttcag aaggtgcaga
      301 tgatgaataa agagagatgc cggacaatcg gaagaaaacg ttacaccaca ggcgcaatgt
      361 gcttcatgct gtttgtgtgg cttggtggtg tgctggctct tgttgctaca gctgcggtgg
      421 taatgcagta aaaaaagggg agcaacatgc tccccgacca gaagaaagac gtttgataac
      481 aattagtgag ttgttacgtc ttgtccagaa tatcatagca acactctgtt gcagtgatac
      541 cgatcgcgat tttagcgaat tccatcataa atcccctgat ttttaagcct gaagcagtca
      601 aaggaatttc tatgccctat atcgatatca ccacgatgcg tgggatgatg ccgcgcgttg
      661 tgacatccat gctgcccgag cattccgctg tactggcgga ggactgccat ttccggtttg
      721 gtgttattac accagaacgt cagatatccg gggttgagaa aacattcaca attaagccaa
      781 aaacaatttt tcattaccgt gacgatttct ggtttgcatg gccggatgtg gtggatgtga
      841 tccgcagtcc gatcgctcag gacccccacg ggcgtattta ctacactgac gggcgttttc
      901 ctaaagtgac ggatgcgacc attgccacaa aaggggacgg gaatcacccg acatcatcgt
      961 atcgtcttgg gatccccgcg ccgacgacag ctcctgtctg tactgtccag cagggcggtg
     1021 atgtttctga cgataacccg aatgatgatg aaacccggtt ttatacggaa acctttgtct
     1081 cagattatgg tgaagaaggt ccgccaggtc cggcgtctct ggaggtaaca ctccgtactc
     1141 cgggaactgc ggtacaactg acgctggctc cggtgccatt gcagaatgcc agtattaaac
     1201 gtcgtcgtat ttatcgttct gcctcagggg gaggagaagc cgatttttta ctcgtggctg
     1261 aactggatgc atccgtgctc agttacacgg ataaaatacc ggcgaaaaat cttggacctt
     1321 cgctggcgac atgggattac ctgccgccgc cggagaatat gacaggcctt tgcctgatgg
     1381 cgaatggtat tgccgccgga tttgccggta atgaagtgat gttttcggaa gcgtatctgc
     1441 cgtatgcatg gccggaagtg aatcgtcaca cgacggcaga agatattgtg gctatctgtc
     1501 cgctcggaac atcactggtg gtggcgacaa agggtgagcc ttatctgttc agtggtgtat
     1561 cgccttccac aatttctggc tccagaattc cttcaatgca ggcgtgtctg agcaggcgga
     1621 gtatggtcgc gatggagggc tttgtgctgt atgcaggaac aaatggcctg gtgtctgttg
     1681 atgcaaacgg taatgccgct ctggcgacgg agcagattat ttcgccggaa cagtggcaga
     1741 gtcagtttaa tccggcctcc attgtggctt atccctggcg tggtgaatac attgcctgtt
     1801 acacgaaacc ggatggtaag caggatgtgt ttgtcttcaa cccggcaggt atggatatcc
     1861 gctacctcag tacacctttt gactgcgcat gtgttgacct ggttaacgat gttatgcggg
     1921 tggtatcagg acaaaacatg tctgcgatcg ccggagggag tttgccgtcg acaatcagat
     1981 ggcattcaaa ggtattttcc cttccggaaa gaacctcttt ctcctgtctc agggtgaagt
     2041 ctccgacgcc agagcgggtg ggaattactg tgctggcaga tgatgttccg gtgattcacc
     2101 ttgcacctgg aagcttttcg ggaagcgttg tgagattacc tgctgcaacc gggcaaaact
     2161 ggcagatact ggtttccggt tttggtcagg ttgaacgtat tacactcagt acatcgatgt
     2221 cggagctgcc ggtatgacaa aaaaaccgtg gcgcgcagga aaggatttat ctgcggttgt
     2281 ggaaaatatg gagataggga caggacaacg tggcgatggt cgtcatgctt ttgtaacccg
     2341 tgaagaactg gtcggtctga agctggcccg tcgccggact tcaggtggtg ccgcatatgc
     2401 ccttaatccg gggattgaga tggacagctc tgtaatggtc gttgatttcc catcgaaacc
     2461 tcagaatttt aaggcgacag ggggatttgg ctcggtactg cttgaatggg atatgccgaa
     2521 ttatcgcggt cattcactga ctgaaatctg gcggggtacg gaggatgacc ttgctgatgc
     2581 cgtgctggt
//
LOCUS       DAZGJT010000235         2586 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZGJT010000235 DAZGJT010000000
VERSION     DAZGJT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.240"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..716)
                     /gene="yecA"
                     /locus_tag="ACHKFK_004856"
     CDS             complement(51..716)
                     /gene="yecA"
                     /locus_tag="ACHKFK_004856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO9054989.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPAQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(1155..1660)
                     /locus_tag="ACHKFK_004857"
                     /pseudo
     CDS             complement(1155..1660)
                     /locus_tag="ACHKFK_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461173.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
     gene            1691..1771
                     /locus_tag="ACHKFK_004858"
                     /pseudo
     CDS             1691..1771
                     /locus_tag="ACHKFK_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
     gene            1865..2107
                     /locus_tag="ACHKFK_004859"
     CDS             1865..2107
                     /locus_tag="ACHKFK_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003840043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO9054990.1"
                     /translation="MFVELVYDKRNFDGLPDAKDIILGELTKRVRRILPNADVRVKPM
                     MTLPSNNTDASKHEKEQISRAVQEMFEEADMWLVSD"
BASE COUNT          659 a          651 c          557 g          719 t
ORIGIN      
        1 caaattcctt attgggtgcc agaattaacg ctgacaccca atgtgacctc ttaatgcagg
       61 cagcactgct taaatttctt accactaccg cacggacaag gatcgttacg ccccggtttc
      121 tcttctgctt tgataggttg ctgaacagct ttttcctgcg gatgcgccat ccagtatgca
      181 tgtaaatcaa gcgccgccag tcgaatagca tctacgctct cttcaaacgc ttctggcgac
      241 atcttctcta cccgctcgaa gttttcctca gtaccgtgca gcgcaatcgc ctccagcgct
      301 ggttttaacg aatcgggcaa cgttgaccag tcagaaagtg ccacgccccg catatagcca
      361 aagcaccact cctcaacaat cgtcagctcg ctgccatcga cttctcgcaa gccgaataac
      421 ggctcaaact gctccgggaa ttcgttcaga cgctctgcgg tatcggccat atgttgaaaa
      481 gccaggttca taaagcgcgt catctctttc tctgacgccc agcgcggcac atagtcagcc
      541 ccaccccaca cggcaaccag ccactgtgcc ggttcaatct cttgcggaga actcaacacc
      601 gccgtcaata aaccgtccag ctccgccaca tcaagaatgg cgtggtcagt gttgtatttg
      661 gtcagaatat cgtccagcca ttccaactca ctttcgttta acggtcccgt tttcatacgc
      721 ttttccttgt ggatctcaac tcgccagcac ctatcttaca tgccggtccg tatcagagat
      781 actttttgag tggctttgcc ggtgattaaa aattaaggag ggtgtaacga caagttgcag
      841 gcacaaaaaa accacccgaa ggtggtttca cgacactgct tattgctttg attttattct
      901 tatctttccc atggtacccg gagcgggact tgaacccgca cagcgcgaac gccgagggat
      961 tttaaatcgt gcgtgttatt atgtattatc aacaagttgc gccgtttttt cgccaataac
     1021 atatgatttt aagatcaact taatcaatag tttataagca tcaagaaggt acaatggtga
     1081 aacatttttt gctctcaaca ccgtcctaac gtgtcgtgta aatgaggtta aaatcctctc
     1141 acaatttaag ctattcacat cgccgggcag tcatcaaact ccgcgttcct ggcgtcatta
     1201 atgatgtaag tgatcacccc gaatatagcg ggtgcagaac tgtagctgtc atcatctact
     1261 ggtagcgact cccttcttcc attctccaga tttatcaagt ggggctgagg gtgagttcgg
     1321 tatcgcttta tcctgaattc cccatctatc gcgcatatca gcagcgagcc atcacaggga
     1381 gaaagtgacg catcaacaac cagtagcgcc ccctgaatta tcccttccct gaaatgcgaa
     1441 cgcgatgccc gcatgaaata agtcgctgct ggctggatga ttagctgctg atcgagggag
     1501 attcgtgttt caacataatc tgcagcaggt gaaggaaagc ccatggctat agtcctccgt
     1561 ttggattgaa cagctgaaag gtacggttct cgccttcctg cgttgataca tcgcggaatg
     1621 ttgtcacata ccactcgatc cattcgttgg cctgcttcat cgtccagttc cagttaacct
     1681 tgttcagttc ctggacaaac cgttgcgtgg ttacggtctt ccggccattt ggttcctgat
     1741 gtatcgaagc atgccaggct atttcaatat cgctgcgtct tggcatcatc acgccctctc
     1801 ttgaataccg gataaaaaca cagtataaat actgtatatc tatccagtaa aggggtaatg
     1861 cgcaatgttc gtggaactcg tttatgacaa aaggaatttt gatggtctgc cagatgcaaa
     1921 agatatcatt ctgggcgaat tgaccaagag ggttcgcagg attttaccca atgctgatgt
     1981 tcgggttaaa ccgatgatga cactgccatc gaacaacact gacgccagca agcatgagaa
     2041 ggaacagata agccgtgctg ttcaggaaat gtttgaagaa gctgatatgt ggctggtttc
     2101 agattaaacg ccttgaactg tcatattgct taagtacaga ccgtcgagac tggccatcat
     2161 tcaatacttg cactatttta gcgcccgcca gtcagcatac atacttgatg ttgcatttat
     2221 caccaatcgt atatgcgctt ttgtgcccac acgaagcctt gcaaaccgat gccaagcttt
     2281 gtgtgtcccg ttttggtctc atgccaaaaa tctacctttc cttgcttctg gtgaggtgac
     2341 ttcatgtttc tccagcacaa tcataaaatt agacacagag ccatgttatg ttaatatatt
     2401 cttctcaatg ataaattgtt ttacaggtaa gtaatgggaa ttattagctc aaccaataaa
     2461 acaaagtccg cattctatga gccatctgtc gatgggctca gagcattagc ggttataata
     2521 gttattcttt ttcatgcagg ctttaaatgg atgcctggag gatacgttgg cgtagatgta
     2581 tttttt
//
LOCUS       DAZGJT010000236         2571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZGJT010000236 DAZGJT010000000
VERSION     DAZGJT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.241"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..740
                     /locus_tag="ACHKFK_004860"
     CDS             <1..740
                     /locus_tag="ACHKFK_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HFO9054991.1"
                     /translation="GGKGYFSYPKPTSLIKTLFGSVKTEDKDIYLDFFAGSGTTAHGI
                     LELNIEDGRKRNFICVQLDEETDKKKQAYKEGYKSIFNITKDRIIKAGKKLKKDNPNY
                     NGDLGFKIFETVHDFRAKDESELTLSNLSFFDDAVLTPEQYDTLLTTWCVYDGSLLTT
                     PIEDIDLNGYKAHLCDGRLYLIAPNFTSEALKALLQKLDADKDFAPNKVVFYGSNFES
                     AKQMELNEALQSYANKKSIDLDLVVRN"
     gene            743..>2571
                     /locus_tag="ACHKFK_004861"
     CDS             743..>2571
                     /locus_tag="ACHKFK_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015974229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO9054992.1"
                     /translation="MSKGFTFEKNLPHQKAGVDSVMNVFVSAIPHQTDHVAIRLLANP
                     ELNLSEQQYYNNIKNVQEFNGIEHSKDNYDAKSNVIDVSMETGTGKTYTYTKTIFDLN
                     KSFGINKFIIIVPTLSIKAGTVNFLKSDALKEHFRDDYGRELKTYVVESQKSSGKNTK
                     SYMPQAIHDFVEASNFNKKYIHVLVINSGMINSKSLTDTYDVGLLDNQFDTPFAALSA
                     VKPFIIIDEPHKFPTGKKTWENIEKFNAQYIIRYGATFSEGYKNLVYRLTAVDAFNED
                     LVKGIDAYIEDIVGDGNANLKFVKSDGKEATFELNENSNKKTFKLVKGESLSKTHSAI
                     HDLTLDALNKSTAVLSNGIELKIGSSINPYSYDQTLADNMMRKAVKEHFKLEKEYLTQ
                     RPRIKPLTLFFIDDIEGYRDGNDIAGSLKTKFEEYVLAEANELLKIEKDEFYRNYLEK
                     TIKDISSVHGGYFSKDNSDKDDKIEQEINEILHDKELLLSLENPRRFIFSKWTLREGW
                     DNPNVFQICKLRSSGSTTSKLQEVGRGLRLPVNEYMCRVKDRNFTLNYYVDFTEKDFV
                     DSLVKEINDSSFKETVPSKFTQELKDKILSQYPELSSRTLMNE"
BASE COUNT          901 a          426 c          483 g          761 t
ORIGIN      
        1 ttggtgggaa aggatatttc tcctacccga agccaacaag tttaataaaa acactatttg
       61 gttcagtaaa aaccgaagat aaagacattt atcttgactt ttttgctggc tctggaacca
      121 cagctcatgg tattctggag ttaaatattg aagacggcag gaaaagaaac ttcatctgtg
      181 ttcaattaga tgaagaaaca gataaaaaga aacaagctta taaagaaggg tataaatcca
      241 tcttcaatat aacaaaagac agaattatta aggcaggaaa aaagctgaaa aaagataatc
      301 ctaattataa tggagatttg ggatttaaaa tatttgaaac tgttcatgat tttcgagcaa
      361 aagatgagtc tgaacttacg ctttctaatc ttagtttctt tgacgatgct gtattgacac
      421 cagagcagta tgacactcta ttgacgacat ggtgcgtgta tgatggtagc ttgctaacaa
      481 caccaattga agatattgat cttaatggct acaaagcaca cttgtgcgat ggtcggctat
      541 atctaattgc gcctaatttc acaagtgagg ctttgaaagc tttacttcaa aagctggatg
      601 cagacaaaga ctttgcccct aacaaagttg tattttatgg cagcaacttc gaaagtgcga
      661 aacagatgga actcaatgaa gcactacaaa gttatgctaa taaaaaatct attgatttag
      721 atttggtggt aaggaactaa tcatgtctaa agggttcaca ttcgaaaaaa atttacctca
      781 tcaaaaggct ggcgttgatt cggtaatgaa tgtctttgta tccgccatac ctcatcagac
      841 ggatcatgtt gcaattcgcc tgttagctaa tccagaactg aatctatctg aacaacaata
      901 ctacaacaac ataaaaaatg ttcaggagtt caatggtatt gagcactcaa aagataacta
      961 cgatgctaaa agcaacgtaa ttgatgtttc tatggagaca ggtacaggta aaacttatac
     1021 ttacaccaaa acaatttttg acctaaacaa atcatttggc attaacaagt ttattatcat
     1081 tgttccgact ctatcgatca aagccggaac cgttaacttt ttaaaaagtg atgctttaaa
     1141 agaacacttt cgagatgatt acgggcgtga actaaagact tacgttgttg aaagtcagaa
     1201 aagttctggt aaaaatacaa aatcatatat gcctcaagct attcatgatt tcgtagaagc
     1261 aagtaatttt aataagaaat atattcacgt tcttgtgatc aactcaggga tgattaactc
     1321 aaagtcttta actgacactt atgatgttgg tttgcttgat aatcaatttg atacgccatt
     1381 tgctgctctt agtgcggtta aaccattcat tatcattgat gagcctcata agtttcctac
     1441 tggtaaaaag acatgggaaa atattgaaaa atttaatgct caatatatca tccgctatgg
     1501 agcaaccttt agtgaaggct ataagaactt agtttatcgt cttacagccg tagatgcatt
     1561 caatgaagat cttgttaaag gtattgatgc atacattgag gatattgttg gtgatggtaa
     1621 tgccaacctc aagttcgtca aatctgacgg aaaagaagct acatttgaac tgaacgaaaa
     1681 tagcaataaa aaaacattta agttagtcaa aggtgaatca ctatctaaaa cgcatagtgc
     1741 gatccatgat ttaacactgg atgccttaaa caagagcact gctgtattga gtaatggtat
     1801 tgaactaaaa attggtagtt caataaaccc ctactcttac gatcagacac ttgctgataa
     1861 catgatgcgt aaagctgtca aagaacactt taagttagaa aaagagtatc tgacacaaag
     1921 gccacgaata aagcctctta ctcttttctt cattgatgat attgaagggt atcgtgatgg
     1981 taatgatatt gccggaagtc tgaaaaccaa gtttgaagaa tacgtattgg cagaagctaa
     2041 tgaactgtta aaaatagaga aagatgagtt ttataggaac taccttgaaa aaactattaa
     2101 ggatatatct tctgttcatg gtggttattt ttcaaaagat aatagtgata aagatgataa
     2161 aattgagcag gaaatcaacg aaatccttca tgacaaagag cttttattgt ctttagaaaa
     2221 tcctcgccgt ttcattttct ctaaatggac gttgcgtgaa gggtgggata acccaaatgt
     2281 ttttcagatc tgtaagctcc gttcgagcgg tagcaccaca tctaaacttc aagaggtagg
     2341 gcgtggttta cgtcttccag tgaatgaata catgtgtcgt gtgaaagacc gcaattttac
     2401 cctcaactat tatgttgatt ttacagaaaa agattttgtc gattctcttg tcaaagagat
     2461 caacgatagt tctttcaaag agactgttcc aagtaagttt actcaagagc ttaaggataa
     2521 aatcctatct caatatccag agctttcatc cagaacactc atgaatgaaa t
//
LOCUS       DAZGJT010000237         2507 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZGJT010000237 DAZGJT010000000
VERSION     DAZGJT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.242"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..66
                     /locus_tag="ACHKFK_004862"
     CDS             <1..66
                     /locus_tag="ACHKFK_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HFO9054993.1"
                     /translation="DAFCRTLTRGSLAIPQCHLLS"
     gene            200..745
                     /gene="cobU"
                     /locus_tag="ACHKFK_004863"
     CDS             200..745
                     /gene="cobU"
                     /locus_tag="ACHKFK_004863"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO9054994.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            742..1485
                     /gene="cobS"
                     /locus_tag="ACHKFK_004864"
     CDS             742..1485
                     /gene="cobS"
                     /locus_tag="ACHKFK_004864"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO9054995.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELLFLLALL"
     gene            1497..>2507
                     /gene="cobT"
                     /locus_tag="ACHKFK_004865"
     CDS             1497..>2507
                     /gene="cobT"
                     /locus_tag="ACHKFK_004865"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO9054996.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYN"
BASE COUNT          543 a          550 c          753 g          661 t
ORIGIN      
        1 gacgcatttt gtcggacgtt aacacgagga agtctggcta ttccacaatg ccatcttctt
       61 tcgtaatgga ttgagaatgc tggattttta attatattac gcgcagtatt gatcgtgtaa
      121 atgttttgcg gtcagcaatg gtgggggatc cattgttctg ttcaatgaat accatcatac
      181 aattgaatca ggagtcggta tgatgatttt ggtgacgggg ggcgcacgga gcgggaagag
      241 tcgccacgca gaggcgctta ttggggactc ttcacaggtt ctgtatatcg ccacctcgca
      301 aatccttgat gacgagatgg ctgcacggat agaacatcat cggcaaggcc gtccggcgca
      361 ctggcgcacg gtggagcgtt ggcaacatct tgatgaatta attcatgcag acattaaccc
      421 gcatgaagct gtgttgctgg aatgcgttac cacaatggtg actaatctgt tgtttgatta
      481 tggcggcgat aaagaccctg atgaatggga ttatcaggcg atggaacagg cgattaatgc
      541 tgagattcag tcgttgattg ctgcctgcca acgttgcccc gcaaaggttg tattagtgac
      601 taacgaagtg ggaatgggga ttgtgccgga gagtcgtctg gcacgacatt ttcgtgatat
      661 tgccgggcgg gtaaatcagc agctggccgc tgcggcaaat gaagtatggc tggtggtttc
      721 gggtattgga gtaaaaatca aatgagtaaa ttattttggg cgatgctctc atttattacg
      781 cgtctgcctg taccgcgtcg ctggtcccag ggactggatt tcgagcatta ttctcgcggt
      841 attattactt ttcctttaat tggattattg cttggcgcga ttagcgggct ggtcttcatg
      901 gtgctgcagg catggtgtgg cgtaccactg gcggcactgt ttagcgtact tgtgctggcg
      961 ctgatgaccg ggggattcca cctagacggc ctcgccgata cctgtgatgg cgtattttct
     1021 gcacgtagcc gcgatcgcat gttggaaatc atgcgtgata gccgtttagg cacccatggc
     1081 ggtctggcat tgatttttgt tgtactggca aagattctgg tattaagcga gttggctctg
     1141 cgtggcgaac cgatccttgc atcgctggcg gcagcatgtg cggtcagccg cgggactgcc
     1201 gcattattga tgtatcgtca tcgttatgcc cgggaagaag ggcttggcaa tgtgttcatt
     1261 ggcaaaattg atgggcgaca aacctgcgtc acactcggct tagccgctat ttttgctgcc
     1321 gtattattgc cgggtatgca tggtgtggct gctatggtgg tgacgatggt ggcgattttc
     1381 atcctcggtc agcttctcaa acgtacgctg ggcgggcaaa ccggcgatac gctgggagct
     1441 gctatagagc ttggtgaact gctcttcctg ctggcactgc tataaagaga cgtgacatgc
     1501 aaacacttgc cgatttactg aatacgatcc ctgctatcga tcctgccgct atgtcgcgtg
     1561 cacaacggca tattgacggg ttactcaaac ctgttggtag cctgggaagg ctggaggcgc
     1621 ttgccataca actggcggga atgccggggt tgaatggcat accgcatgtg ggcaaaaaag
     1681 cggtactggt tatgtgtgcc gatcacggcg tctgggagga aggggtcgct atttccccaa
     1741 aagaagtgac agccattcag gctgaaaata tgacccgcgg aacaaccggc gtgtgtgtgc
     1801 tggcagcaca agcgggcgct aacgtccacg tagttgatgt tggtattgat agtgctgagc
     1861 ctatccccgg gcttatcaac atgcgtgtcg cacgaggtag cggcaatatt gcttcagctc
     1921 cggcaatgag tcgccgtcag gctgaaaagt tgcttttgga cgtcatatgt tatacgcggg
     1981 agctggcaaa aaacggtgtc acgctgtttg gtgtaggtga actggggatg gcaaacacga
     2041 caccggcagc ggcaatagtc agcacaatca ctggccggga tcctgaagaa gtggttggga
     2101 ttggcgcaaa cctgccgaca gataaactgg ctaataaaat tgatgttgtg cgccgggcga
     2161 ttacgttgaa tcaaccaaat ccgcaggatg gcgttgatgt tctggcaaaa gtggggggat
     2221 ttgatttggt cggaatggct ggcgtgatgt taggcgctgc ttcctgcggt ttacccgtgt
     2281 tgctggatgg atttctttct tatgctgctg cgctcgcagc ctgccagatg tctcctgcaa
     2341 tcaaaccgta tctcattcct tctcacctgt cggcagaaaa aggggcgcgt atagcgctgt
     2401 cgcatttggg gctggagcct tatctcaata tggagatgcg tttaggtgag gggagtggcg
     2461 cagctctggc gatgcccatc atcgaagctg cctgtgcgat atataac
//
LOCUS       DAZGJT010000238         2499 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZGJT010000238 DAZGJT010000000
VERSION     DAZGJT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.243"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(230..700)
                     /locus_tag="ACHKFK_004866"
     CDS             complement(230..700)
                     /locus_tag="ACHKFK_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO9054997.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(816..1367)
                     /gene="maa"
                     /locus_tag="ACHKFK_004867"
     CDS             complement(816..1367)
                     /gene="maa"
                     /locus_tag="ACHKFK_004867"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO9054998.1"
                     /translation="MSTEKEKMIAGELYHSADEALSRDRLHARRLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFCCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(1541..1759)
                     /locus_tag="ACHKFK_004868"
     CDS             complement(1541..1759)
                     /locus_tag="ACHKFK_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO9054999.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1785..2159)
                     /gene="tomB"
                     /locus_tag="ACHKFK_004869"
     CDS             complement(1785..2159)
                     /gene="tomB"
                     /locus_tag="ACHKFK_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO9055000.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT          738 a          525 c          564 g          672 t
ORIGIN      
        1 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
       61 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
      121 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatcg
      181 ttgtcatttg accaggcgta gagctttacg cctggtatgt acccctaact taagatgttg
      241 attcggcgcg agcgagggta aaaatatcgt aaaaagatag ttcaccttta cggacgatca
      301 ttttttgcag ttgttcttta tgaacatccg cgacacgcgg agagttcagg atggcttcaa
      361 ctagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
      421 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
      481 taagcaggat aaataataca accagtagcc agacagagcc tgaaagcgtt tcggactgca
      541 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
      601 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
      661 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtgatatct
      721 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatt
      781 tgccacaatt ttgcataaca gttgagaaaa gctggttaca attttttaat gattctggct
      841 ggattaccgc ccacgacaac gttgtccggg acatcttttg tgacaactgc acctgaggct
      901 accacgacgt tatcaccaat ggtcacacca gggttaatga ctgcacgtcc gccaatccag
      961 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     1021 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcacca
     1081 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     1141 agaaaaatgt tatagccata gtcacagcaa aacgttggct caatataagc ctctgtcacc
     1201 tgaccgaata aatcagcaag aatttgctgg cgtaatgtgt gctcttccgc cagggaatga
     1261 ttgtatcggt gaataagccg acgagcgtgc aggcgatcgc gagataacgc ctcatctgcc
     1321 gagtgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacttccgg
     1381 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttcccattac
     1441 atctgtgtga tgaagatcac atagggtatg tcttcatggc gaaaaaaagt ataaaattct
     1501 taataaacag ccggttatag ctccgaaagc gagttattta ttagcgaata aatttccata
     1561 ctgaggaagg gatcttgtcg tacagtttat tcatggtcaa ttcggcgagg cggtgatctg
     1621 cagctgagta aaataccgcc agttcattat ctgataattc gtatttattt ttctcgataa
     1681 cacgctccag cgtgtcaatt gtctgacaac gacgtaaacg cattaaataa tcggttttcg
     1741 ttaaaggttt ttcggacata cttctaccca tggttgtaat aattctaaca agataaactc
     1801 gcaggattct ctttcgtcgc attgacaaaa caacggaata gtcgattacc tgacttccgc
     1861 catttctgaa gatcctgcat attaatacca taactactga acaacataaa ggtgtcatcc
     1921 agatattcgt cgatctgctc aatgagctta ttgtcttcat tatacttaat tttgtaatta
     1981 agtgcgaagg tcgcaatatg ctcaatcagt tcattcaact ggaggttgat cgccgaggtt
     2041 gggtcgttaa cccagccatg attgctttct tcaaggtttg caaggcagtc atgatacagg
     2101 gtttcacaga gaaacttaag ctgtgcgata tcatgtcttt tgggtgagta ttcatccata
     2161 acgcgtcccc ttcttagcgg ttgaactaac ggacaccttt cgggatggaa aaaacttact
     2221 gacctggact tgccctctcg tttgttagct taactataag ccactctttg taggttttca
     2281 tcgcattatt acgaaaaatt acaattagga aggaagcggg acaggactca ctttttcata
     2341 ctagcaccaa cccgcagcag gtaaaagcag tttgctcgaa ctgttcataa catttcagaa
     2401 ttatcgccag aagaaattgg tgacactaat accaggattg ctctgaatat gacgtaataa
     2461 ccgaggaatg aataaagaat taccgcaaat aattaagaa
//
LOCUS       DAZGJT010000239         2495 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZGJT010000239 DAZGJT010000000
VERSION     DAZGJT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.244"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            228..1562
                     /gene="cadB"
                     /locus_tag="ACHKFK_004870"
     CDS             228..1562
                     /gene="cadB"
                     /locus_tag="ACHKFK_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO9055001.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSIDNHTASNAH"
     gene            1642..>2495
                     /gene="cadA"
                     /locus_tag="ACHKFK_004871"
     CDS             1642..>2495
                     /gene="cadA"
                     /locus_tag="ACHKFK_004871"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO9055002.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAADDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHAT"
BASE COUNT          589 a          575 c          578 g          753 t
ORIGIN      
        1 cattgtaaac attgaatgtt tatcttttca tgatatcaac ttgcgattct gatgtgttaa
       61 taaaaaacct caagttctca cttacagaaa cttttgtgtt atttcaccta atctttagga
      121 ttaatccttt tttcgtgagt aatcttagcg ccagtttggt ctggtcagga aatagttata
      181 catcatgacc cggactccaa attcaaaaat gaaattagga gaagagcatg agttctgcca
      241 agaagatcgg gctatttgcc tgtaccggtg ttgttgccgg taatatgatg gggagcggta
      301 ttgcattatt acctgcgaac ctagcaagta tcggtggtat tgctatctgg ggttggatta
      361 tctctattat tggtgcaatg tcgctggcat atgtatatgc ccgactggca acaaaaaacc
      421 cgcaacaagg tggcccaatt gcgtatgccg gagaaatttc ccctgcattt ggttttcaga
      481 caggtgttct ttattaccat gctaactgga ttggtaacct ggcaattggt attaccgctg
      541 tatcttatct ttccaccttc ttcccagtat taaatgatcc tgttccggcg ggtatcgcct
      601 gtattgctat cgtctgggta tttacctttg taaatatgct cggcggtacc tgggtaagcc
      661 gtttaaccac tattggtctg gtgctggttc ttattcctgt ggtgatgact gctattgttg
      721 gctggcattg gtttgatgca gcaacttatg cagctaactg gaatactgcg gataccactg
      781 atggtcatgc gatcattaaa agtattctgc tctgcctgtg ggccttcgtg ggtgttgaat
      841 ccgcagcagt aagtactggt atggttaaaa acccgaaacg taccgttccg ctggcaacca
      901 tgctgggtac tggtttagca ggtattgttt atatcgctgc gactcaggtg ctttccggta
      961 tgtatccgtc ttctgtaatg gcggcttccg gtgctccgtt tgcaatcagt gcttcaacta
     1021 tcctcggtaa ctgggctgcg ccactggttt ctgcattcac cgcctttgcg tgtctgactt
     1081 ctctgggctc ctggatgatg ttggtaggcc aggcaggtgt gcgtgccgct aacgacggta
     1141 acttcccgaa agtttatggt gaagtcgaca gcaacggtat tccgaaaaaa ggtctgctgc
     1201 tggctgcagt gaaaatgact gccctgatga tcctcatcac tctgatgaac tctgccggtg
     1261 gtaaagcatc tgacctgttc ggtgaactga ccggtatcgc agtactgctg actatgctgc
     1321 cgtacttcta ctcttgcgtt gacctgattc gttttgaagg cgttaacatc cgcaactttg
     1381 tcagcctgat ctgctctgta ctgggttgcg tgttctgctt catcgcgctg atgggcgcaa
     1441 gctccttcga gctggcaggt accttcatcg tcagcctgat tatcctgatg ttctacgctc
     1501 gcaaaatgca cgagcgccag agccactcaa tagataacca cacagcgtct aacgcacatt
     1561 aattaaaagt attttccgag gctcctcctt tcattttgtc ccatgtgttg ggaggggcct
     1621 tttttacctg gagatatgac tatgaacgtt attgcaatat tgaatcacat gggggtttat
     1681 tttaaagaag aacccatccg tgaacttcat cgcgcgcttg aacgtctgaa cttccagatt
     1741 gtttacccga acgaccgtga cgacttatta aaactgatcg aaaacaatgc gcgtctgtgc
     1801 ggcgttattt ttgactggga taaatataat ctcgagctgt gcgaagaaat tagcaaaatg
     1861 aacgagaacc tgccgttgta cgcgttcgct aatacgtatt ccactctcga tgtaagcctg
     1921 aatgacctgc gtttacagat tagcttcttt gaatatgcgc tgggtgctgc tgatgatatt
     1981 gctaacaaga tcaagcagac cactgacgaa tatatcaaca ctattctgcc tccgctgacc
     2041 aaagcactgt ttaaatatgt tcgtgaaggt aaatatactt tctgtactcc tggtcacatg
     2101 ggtggtactg cattccagaa aagcccggta ggtagcctgt tctatgattt ctttggtccg
     2161 aacaccatga aatctgatat ttccatttca gtatctgaat tgggttctct gctggatcac
     2221 agtggtccac acaaagaagc agaacagtat atcgctcgcg tctttaacgc agaccgcagc
     2281 tacatggtga ccaacggtac ttccactgcg aacaaaattg ttggtatgta ctctgctccg
     2341 gcaggcagca ccattctgat tgaccgtaac tgccacaaat cgctgaccca cctgatgatg
     2401 atgagcgatg ttacgccaat ctatttccgc ccgacccgta acgcttacgg tattcttggt
     2461 ggtatcccac agagtgaatt ccagcacgct accat
//
LOCUS       DAZGJT010000240         2469 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZGJT010000240 DAZGJT010000000
VERSION     DAZGJT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.245"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..1447)
                     /gene="ydcR"
                     /locus_tag="ACHKFK_004872"
     CDS             complement(41..1447)
                     /gene="ydcR"
                     /locus_tag="ACHKFK_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO9055003.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKATVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDIKEGIDLQALELALQEYPVKACWLMTNSQNPLGFTLTPQKKVRLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLVALTHHISIAPGNMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            complement(1526..1762)
                     /gene="hicB"
                     /locus_tag="ACHKFK_004873"
                     /pseudo
     CDS             complement(1526..1762)
                     /gene="hicB"
                     /locus_tag="ACHKFK_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            complement(2153..>2469)
                     /locus_tag="ACHKFK_004874"
     CDS             complement(2153..>2469)
                     /locus_tag="ACHKFK_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO9055004.1"
                     /translation="GKLHRYCLEALPKRTGTVIAGYNGTTNQDMTMSGGLIVERAPYS
                     RVRRLTPTECERLQGFPDGYTDIPWKNSSPAHRYKAIGNSMPVPVMKWIGRRIQEALN
                     EV"
BASE COUNT          647 a          600 c          555 g          667 t
ORIGIN      
        1 ttcatattac ccaacctgag aggaattaaa ttaaacgcta ttacagccgt tcttgaataa
       61 gtttgcctaa ttgttttacc gcctgttctt cacgttctcc ccactgccac gcggtattaa
      121 aacgaaaaaa acgtgaccag ttttcaccgg tagaaaacat attacccggc gcaatactga
      181 tatgatgcgt cagtgccacc aggcttaatt ctcctgcatc taacggctcg gggagctcca
      241 gccagagaaa ataaccgctg tcattgtgat gaattttgac ttccgcaggc agataacgca
      301 gcaaagcctg ccaggcgcgt tgtttacgtt ccgcaagctg gcgacgcagg cgacgaagat
      361 gagcatcgta tcggcgagta gaaaggtaat ccaccagcgc aagctgcatc ggtgagctgg
      421 tggaaagcgt actcatcaac tgcaagcgtt gaattttacg tgcatgtttt ccggcggcga
      481 cccaaccaat acgaaaacca ggcaccagac atttcgaaaa cgaagagcaa tgcaaaacac
      541 catcgtggcg atcccacgct ttcgcaggca gcggtttttc ccgtccaaaa taaagttcgc
      601 tataaacgtc atcttcaatc agcgttacgt tgtactgatt gagcaacgct accagccgca
      661 cttttttctg cggcgttaag gtaaatccaa gtgggttctg gctattagtc atcagccagc
      721 acgctttcac cggatactcc tgcaacgcca gttccagcgc ctgaagatcg atcccttctt
      781 taatatccgt cgctaccgat aacgccttca gccgtaaccg ctccagcgcc tgcaacgcgc
      841 cgtagaaaca aggattctct actatcaccc aatcgcccgg ttcagttacc gcttgcaaac
      901 tgaggtttaa tgcctctaac gccccggcgg taatgacaat ttcatcagga gaaatggtga
      961 tgccctgtaa ggcatagcga cgagcaatag cctgacgcag ttctgcgttt cctggcggta
     1021 agttttcaat cacgctcatc gccgtggcgg ttttgcttac ctgcgccagc gagcggttta
     1081 gttgttggag ggggaaaagt cgcgggtcgg gaaaggccga ggcaaacgga actaccgacg
     1141 gatcacgact ggcctgcaac atatcaaaaa tataggtgtt gatatcgacg gcttcatctc
     1201 gtgtgactgg aatgactgtc gctttcggca tttttattgc ctgtggcgca acgtaataac
     1261 ccgattgcgg tcgcgcgata atatatccct gactttcgag caactgatag gcatggctga
     1321 cagtcataaa gctcatgcca gaaagcgcca cctggtcacg caacgaaggc aaacgatcgc
     1381 cgggttgcca gatacccgac gcaatctgct cgcgtaattg ttctgcaagc tgctggtatt
     1441 ttttcattat cgaatcgtta ttatgtgcag gtgattcggc agtctatatc agttgttaaa
     1501 aatgacaact tttcattaac tgttatcaaa ccatcaccag cgataactct ttgcctagcg
     1561 cattagccgc gagctggacg gcgtcgattt ttgtcgcatg gtgcaaatta aataggcgag
     1621 taatctcctg tttatgtttg ccaattcgcc tggctaactc ttgctgagtg atttctgact
     1681 gtaaaaaagc atttaacagc aataccttag aggcgacgct caaaggcact tcaataaagt
     1741 gatcgtgact atttaatggc gaagtttacc tccgtgtgta atgtgctcga aaatagcact
     1801 aaatcgccgc aacggcagaa tgcaatgcaa aatcaggaag gaagattcca gaatttcttt
     1861 gctctaattt gcaaaactga ataataacct caagaaacga tcggcatcat agcggcaaaa
     1921 ggttagaaaa aggtggcggg atgttttcga aggcgttatc ggttgtctta ttaacgtgtg
     1981 cactgttcta tagcgcggcg atttttttga taccgatttg catttcattt gataccgatg
     2041 aaaagcgccg ataagcatct tttttgatac taaattggcg ctttcctttg cactccttaa
     2101 agacatcttt taacggcaca aaaaccaact tcataatgtc ttttgcttag gtttaaactt
     2161 cgtttaacgc ttcctgtatt cttcgaccaa tccatttcat gaccggtaca ggcattgaat
     2221 ttccaatggc cttataacga tgggctggag atgaattttt ccatggaata tctgtatatc
     2281 catcgggaaa accttgtagc ctctcgcatt cggttggcgt caacctacga acccttgaat
     2341 atggcgctct ttcaactatg agcccaccag acatagtcat gtcctggtta gttgttccgt
     2401 tatagccagc tattactgtg cctgttcgtt ttggcaatgc ttcaaggcaa tatcggtgca
     2461 attttcctt
//
LOCUS       DAZGJT010000241         2448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZGJT010000241 DAZGJT010000000
VERSION     DAZGJT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.246"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            78..587
                     /gene="eco"
                     /locus_tag="ACHKFK_004875"
     CDS             78..587
                     /gene="eco"
                     /locus_tag="ACHKFK_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO9055005.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(737..2383)
                     /gene="mqo"
                     /locus_tag="ACHKFK_004876"
     CDS             complement(737..2383)
                     /gene="mqo"
                     /locus_tag="ACHKFK_004876"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO9055006.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNSWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLNLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          584 a          665 c          610 g          589 t
ORIGIN      
        1 taagttattc ataaagcaaa ttaataaatc tgatgaatat gttaaccttc agcgacatca
       61 tcggtgaaaa cctataaatg aagaaggaaa gcaaaaaaat gaagaccatt ctacctgcag
      121 tattgtttgc cgctttcgcc accacttccg cctgggcggc agaaagcgtc cagccactgg
      181 aaaaaatcgc accttatcca caagctgaaa aaggcatgaa gcgtcaggtg attcagttaa
      241 ctccgcaaga agatgaatct accctgaaag tagaactgtt aatcggtcag acgctggaag
      301 tcgactgcaa tttgcatcgt ctcggcggga agctggaaag caaaacgctg gaaggctggg
      361 gctatgatta ttatgtcttt gataaagtta gctcgccggt ttccacgatg atggcctgcc
      421 cggatggcaa gaaagagaag aaatttgtca ccgcgtatct gggcgatgct ggaatgctgc
      481 gttacaacag caagctgccg atcgtggtgt atacgccaga caatgtggat gtgaagtacc
      541 gtgtctggaa ggcggaagag aaaattgaca acgcggttgt tcgctaaact gctgtgaagt
      601 gctgcaacct gtaggccgaa taaggcggtt acgccgcatc cgacatctaa cgcccgagcc
      661 ggttgcctga tgcgacgctg aagcgtctta tcagcatacg ccacatccgg caaaccatgc
      721 cggatgtggc acatcattac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt
      781 tcaactgcgg tttcggcgta ctatctgctg cctgcggctt gtcgtatttc agccccagca
      841 cttcgctggt gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc
      901 cataagacgg aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc
      961 caaatacttt ttccagcaga ttcaacataa tcggcgcggc ggttgacgcc cctggcgatg
     1021 cccccaggag cgcggcaatg gtcccttgct ggtcactgac gacttcagta cccagacgca
     1081 gtacgccacc tttatcggca tcacgcttga taatctgcac gcgctgcccc gcttgccaca
     1141 aacgccagtc ctcttttttc gcctgcggat agtactcttt caacgcttca aaacgatcct
     1201 cttcactcaa catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccaa
     1261 cgtgcatcat cggcatcacg ttagaggtgg tggtggaact catcagatcc cataatgaac
     1321 cgtttttgag gaatttggtt gagaaggtgg caaatggccc aaacagcact acgcgtttac
     1381 cgtcaagaac acgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt
     1441 taccgtaaac cttcgccaga tggtgattaa ccacgtccgg gttttccgaa acaaggaact
     1501 gtccgcccac cgggaaaccg gcgtagtctt tcgcctccgg aatccccgat tcctgtaaca
     1561 gtttcagcgc cgcaccgccc gcgccgataa agacaaattt ggcgcggatg ttctgtgcag
     1621 tgccgttttt cagatcggca acggtgacgg tccagctgtt atcgtcatta cgctttaggg
     1681 cgcggacttc gctgctgagt tgcagcgaga agttagattt cttctgtaag gaggcgatca
     1741 actggcgggt gatctcgcca tagttcacat cagtgccaat ttctgtgcgt gttgccgcta
     1801 ctttctgttg cggatcgcgc ccttccatca ctaacggtgc ccactctttg atctgcgcgt
     1861 gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg
     1921 cgcgcaggaa attaacgtta tcctcgcccc agacaaagct catatgcgga acggtattga
     1981 taaatgaacg cggagtacgc agcacaccgc gctcgacctg gtgcgcccag aactggcggg
     2041 aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg ccgtcggcat
     2101 tttgcggggt gtaattgagt tccatcagtg cagaatgccc ggttccggcg ttgttccagc
     2161 cgttcgaact ctcctgcgcg acaccttcca ggcgctccac catggtcatc gaccattcag
     2221 gctccagctc gcgtaaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca
     2281 gtacatcagt ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa
     2341 gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta atgccttact
     2401 tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggtaaca
//
LOCUS       DAZGJT010000242         2430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZGJT010000242 DAZGJT010000000
VERSION     DAZGJT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.247"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..270
                     /locus_tag="ACHKFK_004877"
     CDS             <1..270
                     /locus_tag="ACHKFK_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9055007.1"
                     /translation="WLTTRQSKPVLTEGMKTLLNNGLSGIRWSGTLSEMNTYVYDAKG
                     SMNAQEGCFDDQLMSYMIAQEMRARMPVRVKQKTDKRRTTHWMAH"
     gene            270..2414
                     /locus_tag="ACHKFK_004878"
     CDS             270..2414
                     /locus_tag="ACHKFK_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HFO9055008.1"
                     /translation="MKNETNTMATKNDNGATPRFSQRQLQALCSDIDSQPKWRDAANK
                     ACAYYDGDQLPLEVLQVLKDRGQPMTIHNLIAPTVDGVLGMEAKTRTDLLVISDDPDD
                     ETEKLAEAINAEFADACRLGNMNKARSDAYAEQIKVGLSWVEVRRNSDPFGPEFKVST
                     VSRNEVFWDWLSREADLSDCRWLMRRRWMDTDEAKATFPGMAQVIDYAIDDWRGFVDT
                     TVTEGQPSPLMSAWEEYQSWDRKENEWLQRERRRVLLQVVYYRTFDRLPVMTLSSGRV
                     VAFDKNNLMHAVAVASGRVQVRVGRVSRIREAWFVGPHFIVDRPCSAPQGMFPLVPFW
                     GYRKDKTGEPYGLISRAIPAQDEVNFRRIKLTWLLQAKRVIMDEDATQLSDNELMEQI
                     ERPDGIIKLNPARKNQKSVADVFRVEQDFQVASQQFQVMQESEKLIQDTMGVYSAFLG
                     QESGATSGMAISNLVEQGATTLAEINDNYQFACQQVGRLLLAYLLDDLKKRRNHAVVI
                     NRDDRQRRQTIVLNAEGDNGELTNDISRLNTHIALAPVQQTPAFKAQLAQRMSEVIQG
                     LPPQVQAVVLDLWVNLLDVPQKQEFVERIRAALGTPKSPDEMTPEEQQAAQQQQALEQ
                     QQQELQMREMAGRVAKLEAEAARAQAAAQRDNAGAQRDVAAAQGQRYVDALNQAHTAE
                     IITGIQNMEQEQEVLQQQMLHTLQQRMHELPL"
BASE COUNT          614 a          565 c          722 g          529 t
ORIGIN      
        1 tggctgacaa cccgccagag taaaccggtc ctgacagagg gaatgaaaac gctgctgaat
       61 aacggcctgt cggggatccg ctggtcgggc acattatcag aaatgaacac ctacgtttat
      121 gacgcgaaag gctccatgaa tgcacaggaa ggctgttttg atgatcagct tatgagctac
      181 atgattgccc aggagatgcg cgccagaatg ccggtgaggg taaaacagaa aacggataaa
      241 cgcagaacca cacactggat ggctcactga tgaaaaatga aactaatacc atggcgacga
      301 aaaacgacaa cggagccacg ccgcgttttt ctcagcgcca gttacaggcg ctttgttctg
      361 atattgacag tcagcctaaa tggcgcgatg ccgcaaacaa ggcctgtgcg tattacgatg
      421 gtgaccagtt gccactggaa gttctccagg tgttgaaaga tcgcggtcag ccgatgacca
      481 tccataacct catcgcgcct accgttgatg gtgttctggg tatggaggcc aaaacgcgga
      541 ctgatctgtt ggtgatatca gatgatcctg atgatgaaac agaaaaactg gctgaagcta
      601 ttaatgctga atttgccgat gcttgccgcc ttggaaatat gaataaagcc cgttctgatg
      661 cctatgcgga acaaatcaaa gtggggctca gctgggtgga ggtcagacgg aacagcgatc
      721 cgttcgggcc tgagtttaag gtgtctactg tcagccggaa tgaagtgttc tgggactggc
      781 tcagccggga ggctgattta agtgactgcc gctggctgat gcgccgccgc tggatggata
      841 ccgatgaggc aaaagccaca tttccgggaa tggctcaggt tatcgattat gccattgatg
      901 actggcgtgg ttttgttgac accacggtta ctgaaggcca gcccagtcct ttgatgagtg
      961 catgggaaga gtaccagtca tgggatcgta aggagaacga atggcttcag cgtgaacgcc
     1021 gccgcgtgct tcttcaggtg gtttattacc gcacattcga tcgcctgcct gtgatgacgc
     1081 tcagcagtgg cagggtggtg gcctttgata aaaataatct gatgcatgcg gtggcggtgg
     1141 catccgggcg ggtgcaggtg agagtcgggc gggtaagtcg tattcgtgaa gcctggtttg
     1201 tggggccgca ttttattgtg gatcgcccct gtagtgcgcc acaggggatg tttccgctgg
     1261 ttcccttctg gggataccgg aaggataaaa ccggagagcc ttacgggctg atttcccggg
     1321 ccattccggc acaggatgag gtgaattttc gtcgtataaa gctgacgtgg ttgcttcagg
     1381 ccaaacgtgt gattatggac gaggatgcca cccagttgtc agacaacgag ctgatggagc
     1441 agatcgagcg tccggatggc attattaaac tgaacccggc ccgaaaaaat cagaaaagcg
     1501 ttgctgatgt tttccgggtt gaacaggatt ttcaggttgc cagccagcag tttcaggtta
     1561 tgcaggagtc ggaaaaactt atccaggaca cgatgggggt ttattccgca tttctcgggc
     1621 aggaatcagg cgcaacttcc ggcatggcga tcagcaacct ggtggagcag ggagccacaa
     1681 cccttgccga aatcaacgat aactaccagt ttgcctgcca gcaggtggga aggctgttgc
     1741 tcgcttatct tctcgatgac ctgaaaaagc ggcgtaatca tgcggtggtg attaatcgcg
     1801 atgatcgcca gcgtcgccag accattgttc tcaatgctga aggtgataat ggtgaactga
     1861 ccaatgatat ttcaaggtta aatacacata ttgcgctggc tcctgttcag cagacaccgg
     1921 cgtttaaggc acagcttgcg cagagaatgt cagaggttat tcaggggctg ccacctcagg
     1981 tgcaggccgt cgtgctcgac ctgtgggtta atcttctgga tgtgccgcag aaacaggaat
     2041 ttgtcgaacg tattcgtgcg gcgctgggga caccaaaatc accggatgag atgaccccgg
     2101 aagagcagca ggccgctcag cagcaacagg cgctggagca gcaacaacag gaactgcaga
     2161 tgcgcgaaat ggcaggccgg gtggctaaac tggaagcaga agctgccagg gcacaggcag
     2221 ctgcgcaacg ggataatgct ggcgcacaac gggatgtcgc cgcggcacag ggacagcgtt
     2281 atgtggatgc gctcaaccag gcgcatacgg cagaaatcat taccggcata cagaatatgg
     2341 aacaggagca ggaagttctt cagcaacaga tgcttcatac actacaacag cggatgcatg
     2401 aattgccgct ctgaatatcc tgaacttaac
//
LOCUS       DAZGJT010000243         2332 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZGJT010000243 DAZGJT010000000
VERSION     DAZGJT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.248"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..689)
                     /locus_tag="ACHKFK_004879"
     CDS             complement(<1..689)
                     /locus_tag="ACHKFK_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HFO9055009.1"
                     /translation="MSVVVSGTLKSPDGQVVSDANITLTALTVSPDALSGTSASAVTT
                     STGYYGMTMEPGEYAVSVTVRGKTAVYGRVRIEGTESTVTLNMLLRRSLVEVSIPGEL
                     LTDFRQIQNNVADDLATIRRLNEDTAVKNNQASQSKESAAASAKTASESAKAATSKAT
                     ESAQKASEAAEAATRAGESEKAAAADAKEARQHAETARVAQEAAGDVLKRAEAATVSA
                     EEARRMAENARG"
     gene            complement(686..1336)
                     /locus_tag="ACHKFK_004880"
     CDS             complement(686..1336)
                     /locus_tag="ACHKFK_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6682 family protein"
                     /protein_id="HFO9055010.1"
                     /translation="MTTITEIIGRVNTQLVDPMMVRWPLAELCDYYNDAVRAVILARP
                     DAGASLETLNCVPGARQTLPDGAIQLLDVICLSDGSAIKPQSREVLDAQYPDWHMLRG
                     KPECFISSDLAPRVFWLFPAPEEAVSVDAVVSRIPEAVYVLTQDDDTPVPLEEAYVNP
                     LVDWMLFRAFSKDAAGGAESGLAAQHYQSFVEQLGIKQGADSTLSARKKVFNGGEM"
     gene            complement(1336..1899)
                     /locus_tag="ACHKFK_004881"
     CDS             complement(1336..1899)
                     /locus_tag="ACHKFK_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055011.1"
                     /translation="MAELTDFLPYVRRHISGPLSIMMTDALSMSAVAFCRQSLLCRRE
                     ATLSPSAGEDCVLPYDAENEECVHIIRILADGRELFAGHDVEIRPGRVMHFACSPGEV
                     SVLYAVAPKAGSRQVQDELRAWSEEVAAGALERLFMQTGVSWSDPSRAQYFSVLFSEG
                     IRRAYRDTLATSPYSSYRNPVRRQRFY"
     gene            complement(1883..>2332)
                     /locus_tag="ACHKFK_004882"
     CDS             complement(1883..>2332)
                     /locus_tag="ACHKFK_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055012.1"
                     /translation="IAVVYIGPKPVKKDTLTGSRTLFPRLEPVHVDSALAWQLLAFPD
                     VWVRHEELDGVLKKQQQDEQLRQAQQAQEREQVALAEAENSFVVSVGGQDVDLSKLTS
                     ARLATLCEAEELNIHKDPKETADAFRVRVREAFRRRVAETEQHGGTD"
BASE COUNT          526 a          747 c          539 g          520 t
ORIGIN      
        1 cctcgtgcat tctcagccat acgtctggct tcttcagcac taaccgtagc agcctcagca
       61 cgtttaagaa cgtctccggc ggcctcctga gccactctgg cggtttcagc atgttgtctg
      121 gcctctttcg cgtctgctgc cgctgccttt tcagactcac ctgcccgggt cgccgcctct
      181 gcagcctcac tggctttttg tgcagactcc gtcgctttgc tggtcgcagc cttcgcgctt
      241 tctgatgcag tcttcgcact ggcagcagcg ctttccttcg actgtgaagc ctgattattt
      301 ttcaccgccg tgtcttcgtt cagacgacga atcgtggcaa ggtcatccgc cacattgttc
      361 tgtatctgcc ggaaatctgt cagcagttct ccgggtatgc tcacctcaac aagactgcgg
      421 cgtaacagca tattgagcgt caccgtactt tcagtcccct caatacgcac acgtccgtag
      481 acagcagtct tccctctcac cgtcactgaa accgcatact cccccggctc catcgtcatg
      541 ccgtaatagc ctgttgacgt ggtgactgcc gacgcgctgg tgccactgag tgcatccggt
      601 gaaacagtca gcgccgtcag cgtaatgttc gcatctgata caacctgacc atcaggcgat
      661 ttcagcgtcc ccgaaacaac aacactcaca tttcacctcc gttaaacacc ttcttgcggg
      721 ccgacaatgt actgtcagcc ccctgtttaa tcccgagctg ctcaacaaaa ctctgataat
      781 gttgtgctgc cagtcctgac tctgcaccac cggcagcatc cttactgaat gcgcggaaca
      841 acatccagtc aaccagcggg ttaacataag cctcttccag cggtacaggc gtgtcatcat
      901 cctgcgtcag aacatacacc gcctccggaa tgcggctgac cacagcatca acactcacag
      961 cctcctccgg tgccggaaac agccagaaca cgcgcggggc aaggtcactg ctgataaagc
     1021 attccggttt ccccctcagc atgtgccagt cgggatactg tgcatcaagc acctcacggg
     1081 attgcggttt tatagcacta ccatcgctga ggcatatcac gtcaagaagc tgtattgcgc
     1141 catcaggcaa agtctgacgt gcaccaggaa cacaattcag cgtctccaga cttgcgcctg
     1201 catccggcct tgccagaatc accgcccgca cagcatcatt gtaataatca cacagctcag
     1261 ccagaggcca gcgcaccatc atcgggtcaa ccagttgcgt gttcactcgt ccgatgattt
     1321 cagtaatcgt cgtcatcagt aaaacctttg tctgcggaca ggattgcggt atgaggagta
     1381 cggacttgtc gccagtgtgt cgcgatatgc ccgacggata ccctcagaaa acagcacaga
     1441 gaaatactgt gcgcgcgacg ggtctgacca tgaaaccccg gtctgcatga acaaccgttc
     1501 aagcgcccct gcagccactt cttcagacca tgcccgcagt tcatcctgca cctgacggct
     1561 ccctgctttc ggagcaacgg cataaagcac actcacctct ccgggcgaac aggcaaaatg
     1621 cattacccgc cccgggcgga tttccacatc atgaccggca aaaagctcac gcccgtcagc
     1681 gaggatacga ataatatgta cgcactcttc attctccgcg tcatatggca gcacacagtc
     1741 ctctccggct gacggtgaca gcgtggcttc acggcggcac aacagggact gacggcagaa
     1801 tgccactgcc gacatggata atgcatccgt catcataatg ctcagcggac cactgatatg
     1861 gcgacggaca tacggtaaaa aatcagtcag ttccgccatg ctgttcagtc tccgcaacac
     1921 gacggcgaaa tgcctcacgc acacggacac gaaatgcatc agccgtttct ttcgggtctt
     1981 tgtgaatatt cagctcttct gcctcacaca acgttgccag ccgtgcggac gtgagtttgc
     2041 tcaaatccac atcctgacca ccaacgctaa ccacaaaact gttctccgct tctgcaagag
     2101 caacctgttc tctttcctgc gcctgctgtg cctgccgcaa ctgctcatcc tgttgttgct
     2161 ttttgagcac accatcaagc tcttcatgac gaacccagac atccggaaag gccagcaact
     2221 gccaggcaag cgcgctgtca acatgcaccg gctcaagacg cgggaacagt gtacgactcc
     2281 cggtaagggt atcctttttc acgggtttgg ggccgatata gacaacggca at
//
LOCUS       DAZGJT010000244         2217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZGJT010000244 DAZGJT010000000
VERSION     DAZGJT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.249"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..306
                     /locus_tag="ACHKFK_004883"
     CDS             <1..306
                     /locus_tag="ACHKFK_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9055013.1"
                     /translation="SDRWVVEQMSQLTAMTASFVLATPTETDGALFPGRIMLANTCMW
                     TYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNVGNFGGFLSINKLSQ"
     gene            312..1055
                     /locus_tag="ACHKFK_004884"
     CDS             312..1055
                     /locus_tag="ACHKFK_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9055014.1"
                     /translation="MTETESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFAHGVTDCYTLFRDAYHLAGIDMPDFHRGDDWWCNGQNLYLDNMEAT
                     GFYRVPLSSALPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            1052..1633
                     /locus_tag="ACHKFK_004885"
     CDS             1052..1633
                     /locus_tag="ACHKFK_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO9055015.1"
                     /translation="MNTAAEAIRALSMQVPGFRRQMNEGWYQIRIAGEDTAPEAVYAR
                     LHEPLGEGTVIHIVPRLAGAGKGGLQIVLGTAAIVGSFLTAGASMALWGTALSAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGGGKVVVIGRQG"
     gene            1975..>2217
                     /locus_tag="ACHKFK_004886"
     CDS             1975..>2217
                     /locus_tag="ACHKFK_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055016.1"
                     /translation="MGKGGGKAHTPREAKDNLKSTQMMSVIDAVGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGF"
BASE COUNT          488 a          549 c          709 g          471 t
ORIGIN      
        1 agtgaccgct gggtggtgga gcagatgtcg cagctgacag ccatgacggc ctcgtttgtg
       61 ctggccacac cgaccgagac ggacggggcg ctgtttcccg gtcgtatcat gctggcgaac
      121 acctgtatgt ggacctaccg ctctgatgag tgtggttaca cgggcggggc tgtggcggat
      181 gagttcgata aacccaccac ggatatccgt aaggacagat gcagcaagtg catgcgcggg
      241 tgtgaactgc gcaggaatgt cggcaatttt ggcggtttcc tttccattaa taaactttcg
      301 cagtaaatcc catgacagag acagaatcag cgattctggc gcatgcccgg cggtgtgcgc
      361 ctgcggagtc gtgcggcttc gtgataagca cgccggaggg ggagcggtat atcccttgtg
      421 tgaatatttc cgcagagccg gaggcgtatt ttcgtatcgc accggaagac tggctgcggg
      481 cagagatgca gggggagatt gtggcactgg tccacagtca tcccggtgga ctgccctggc
      541 tgagcgaggc cgaccggcgg ctgcagataa aaagcgcact gccctggtgg ctggtcagcc
      601 ggggtgacat tcacaaattc cgctgcgttc cgcacctgac gggacggcgc tttgcgcacg
      661 gggtgacgga ctgttacacg ctgttccggg atgcatacca tctggcaggc attgatatgc
      721 cggattttca tcgcggggat gactggtggt gcaacggtca gaacctttac ctggacaata
      781 tggaggcaac gggcttttac cgggtgcccc tgtcctctgc actgccgggc gatatcctgc
      841 tgtgctgctt cggcgcatcg gtggctaatc atgccgccat ttactgcggc aacggtgagc
      901 tgcttcacca tctgcctgaa caactgagta aacgggagag gtattccgaa aaatggcaac
      961 gacgaacgca ttctgtctgg cgtcaccgcc actggcacgc atctgccttc acggggattt
     1021 acaacgattt ggccgccgcc tcagcctgta tgtgaacacg gcagcggaag ccatccgcgc
     1081 actgtcgatg caggtgccgg gctttcgccg tcagatgaac gaaggctggt accagatacg
     1141 cattgccggt gaggatacgg caccggaggc ggtgtatgcc cgccttcacg aaccactggg
     1201 tgagggaacg gttatccata ttgtgccgcg actggccggg gccggaaagg gtggactgca
     1261 gattgtgctg gggacggcag ccatcgtggg ctctttcctc actgccggtg cctcgatggc
     1321 gttatggggt acagccctga gtgccggcgg tttttctgcc accacgatgc tgttttcact
     1381 gggggccagc atgattctgg gtggtgtggc ccagatgctg gccccgaagg caaaaacacc
     1441 ggattaccgc gcaacggata acggcagaca gaacacgtat ttttcgtcac tggacaacat
     1501 gattgcccag ggtaacccga tgccggtgcc ttacggggaa atgctggttg gctcccgccg
     1561 tatatcccag gacatcagta cccgtgatga aggcgggggc ggaaaggtcg tggttatcgg
     1621 gcggcagggg taaaaagaat aaaaaaaatc ccgcagtgat cgcggagctg cggggacaga
     1681 caaagattag agttaaggag ttgtttttgt tactcgggca aaaaaacact aacgcagcga
     1741 aattatacgc gccacagtca gtttgtgaaa atgtgaagat attcagaatt tttatgccat
     1801 taccggtttt taccaacagg attatcggtg gacatgaaag aaaaccccgg tatctgctga
     1861 taccggggtt tctctttagc atggcagaaa tgtgtttcat gcttttcggg cgaaggatat
     1921 ccgacttctg tacggaatgg caagtggcgg ttaatttatt caggggaagg ctgtatggga
     1981 aaaggtggcg gtaaggcaca cacgcctcgt gaggcgaagg ataatctcaa atccacgcag
     2041 atgatgagcg tgattgatgc ggttggtgag ggaccgatag aaggcccggt gaagggactg
     2101 cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc cgtgatacac
     2161 ggtgtgacgg cggtctggcg cgccggggag caggagcaga caccaccgga aggcttt
//
LOCUS       DAZGJT010000245         2143 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZGJT010000245 DAZGJT010000000
VERSION     DAZGJT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.250"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..743)
                     /locus_tag="ACHKFK_004887"
     CDS             complement(84..743)
                     /locus_tag="ACHKFK_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO9055017.1"
                     /translation="MIKIENLTKSYRTPTGRHYVFKNLNIIFPKGYNIALIGQNGAGK
                     STLLRIIGGIDRPDSGNIITEHKISWPVGLAGGFQGSLTGRENVKFVARLYAKRDELN
                     ERVDFVEEFSELGKYFDMPIKTYSSGMRSRLAFGLSMAFKFDYYLIDEITAVGDAKFK
                     KKCSDIFDKIREKSHLIMVSHSERALKEYCDVAIYLNKEGQGKFYKNVTEAIADYKKD
                     L"
     gene            complement(740..1516)
                     /locus_tag="ACHKFK_004888"
     CDS             complement(740..1516)
                     /locus_tag="ACHKFK_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO9055018.1"
                     /translation="MARSGFEVQKVTVEALFLREIRTRFGKFRLGYLWAILEPSAHLL
                     ILLGILGYVMHRTMPDISFPVFLLNGLIPFFIFSSISKRSIGAIEANQGLFNYRPVKP
                     IDTIIARALLETLIYVAVYILLMLIVWMTGEYFEITNFLQLVLTWSLLIILSCGVGLI
                     FMVVGKTFPEMQKVLPILLKPLYFISCIMFPLHSIPKQYWSYLLWNPLVHVVELSREA
                     VMPGYISEGVSLNYLAMFTLVTLFIGLALYRTREEAMLTS"
BASE COUNT          683 a          457 c          359 g          644 t
ORIGIN      
        1 cacccgcacc aaatattatt aactttttac tcatgatatg tctcagttaa atatttccaa
       61 ggcataatat tggaatgtaa ccactatagg tcttttttgt aatcagcaat ggcttccgta
      121 acatttttat aaaatttacc ttgcccctct ttgttaagat aaatagcaac atcacaatac
      181 tcctttaaag ctcgttcact atgtgacacc attattaaat gagatttttc tcttatttta
      241 tcgaatatat ctgaacattt ctttttaaac tttgcgtctc caacagcagt gatttcatca
      301 ataagataat agtcgaattt aaaagccata cttaatccaa aagctaacct tgacctcatg
      361 ccagaagaat aagttttaat gggcatatcg aaatattttc cgagttcgga aaactcttca
      421 acaaaatcaa ccctctcatt taactcatcg cgttttgcat atagtcgggc aacaaattta
      481 acattttcac gaccggttaa acttccttga aatccaccag ccaatccaac gggccatgaa
      541 attttatgtt ctgtaataat gttcccacta tcaggacgat ctataccacc aattatccga
      601 agtaaagttg atttaccggc tccattttga ccaatcaggg caatgttata gcctttagga
      661 aaaataatat tcaaattttt aaaaacataa tgccgcccag tgggcgtgcg ataagacttc
      721 gtcaaattct caatcttaat catgatgtca gcattgcctc ttcacgcgtt cggtataatg
      781 ccaggccgat gaacagggtg accagagtaa acattgcaag gtagttcaga ctcacgcctt
      841 cactgatata gccaggcata actgcctcgc ggcttaactc cacaacatgc actaatgggt
      901 tccagagtag atatgaccag tattgctttg gaatcgagtg tagagggaac atgatgcagg
      961 agatgaaata cagcggctta agcagtatcg gcaggacctt ttgcatttca ggaaaggttt
     1021 taccaacgac cataaatatt aagccgacgc cacatgaaag aatgattaac aaactccagg
     1081 tgagcacaag ttgtaaaaag tttgtaattt cgaaatattc gcctgtcatc cagacgataa
     1141 gcatgagcaa aatataaaca gcaacgtaaa tcagtgtctc aagcagtgca cgtgcaatga
     1201 tcgtatcgat gggttttact ggtcgataat taaacagtcc ctggttcgct tcaatagcac
     1261 caatagaacg tttgctaata ctactaaaga taaaaaaggg aatcaggcca ttaagtaaaa
     1321 acaccgggaa cgagatgtct ggcatagtgc ggtgcataac gtaacccaaa attcccaaca
     1381 atatcagcaa atgcgcggag ggttcaagaa tcgcccacaa ataccccaga cgaaacttac
     1441 caaagcgtgt tcgtatttct cgtagaaata atgcctctac ggtgactttc tgaacttcaa
     1501 atccacttct tgccatttga tgatgtgatc ctaatctctt caggtacgct accgcccttg
     1561 gcttaacagc taccaataca ctggaggtaa tacttgtttg taactggata aaatttactc
     1621 agacggtttg acctttttaa tagccgcaaa aaaatttcag cagaaattat ttccgcaggg
     1681 ataaatagat ctatttccgt ctcgtttctt ctaatccatt cttcaggcat gaatgtattc
     1741 ggtcttctgg taatttcaga aaactggccg ttttagaatg tagagttaat ttctcaggaa
     1801 attatttcca cctctttttg cttaaccaat atggcttgaa aaagacgtaa agcagcatgc
     1861 gttctggatt actccggaac acacttattc aaaatgggct gctaatttcg caagaaatta
     1921 ttttttacta ttactgttaa ctacttgatt ataataccac ctatgaattt tatgcgtaac
     1981 aacacctgca atctgtctca aaccagaacg cacgcgattg attaccaata aattgtttag
     2041 tgagaaacta tttccctatt taaaattcac tcatgtactt cttatttata tctacagccc
     2101 cctctttaca gtcacatttg tgatttacat cacatttaaa cgg
//
LOCUS       DAZGJT010000246         2114 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZGJT010000246 DAZGJT010000000
VERSION     DAZGJT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.251"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1085)
                     /locus_tag="ACHKFK_004889"
     CDS             complement(<1..1085)
                     /locus_tag="ACHKFK_004889"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055019.1"
                     /translation="MFLPLRFNKILSLFLLSSCSFNVMAFSDVDKGGQIRATGFQSEL
                     SGILTTRLLSLGADAATVAFVVKAYSQTAADFVVSYAARSGVAIGSMTWANLAYGLGV
                     TAASVGLYALGKPVAESFARMALSANGYDVTFATPAMTASDHNYWCGIISNGSGVYST
                     SPWSALARMKDAKYPGYSVSYSSLESRTATWYLALPGKPDDPGAVIQVGFNSGCPLDL
                     LNGSDDYPLCDNGEIAVQSEGATAYACEKATTEPVSFTGGTGGLTLDSSSVADVLNAL
                     SREAGQAGYVNGSGLTFTSEDVDSWRSHNDFSSDSTGFTDTSSGLGLPATSQGDTADS
                     GGTYSPPVAGAGAGAGTGTGTTTGTGTT"
     gene            complement(1255..1512)
                     /locus_tag="ACHKFK_004890"
     CDS             complement(1255..1512)
                     /locus_tag="ACHKFK_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055020.1"
                     /translation="MFYKFKSSCGSFKSKVLAVPVLFASGQVFAAGTTTTTGSVDFTP
                     LTTSINFTSVVSVILSIGAAAVTVYLAVIGVRAVWRQIKSI"
     gene            1554..1751
                     /locus_tag="ACHKFK_004891"
     CDS             1554..1751
                     /locus_tag="ACHKFK_004891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055021.1"
                     /translation="MPIKKKNNGNNAPTLKAPGLTEGTSEKSAQTTDIGLLFCTEFSE
                     KLLLPLPDGCGEDDEEKRPQL"
     gene            complement(1799..>2114)
                     /locus_tag="ACHKFK_004892"
     CDS             complement(1799..>2114)
                     /locus_tag="ACHKFK_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055022.1"
                     /translation="QLDPKSAYFDAREKPKYAIVLAYPYEDRKLKIRDTAYEKFSCTE
                     DEYKHFNSCSDLHGKEVYICVDVNSWAVSAERSGVWYRYQSGTMKRFDGLPLDAPSGK
                     EK"
BASE COUNT          738 a          523 c          412 g          441 t
ORIGIN      
        1 gttgttcctg tgccggtggt tgtccctgtg ccagtgcctg cccctgcccc agcgcctgca
       61 acaggcgggc tgtaagtccc gccggaatca gcggtatctc cctgtgatgt tgcaggcaac
      121 cccaggccgg aagatgtatc cgtaaatcca gtagaatcag atgaaaaatc attatgcgaa
      181 cgccaggaat caacatcctc cgacgtaaat gtcagaccac tgccattaac atagccagcc
      241 tggccagcct cacgcgaaag tgcattcagt acatccgcaa cagacgaaga atccagcgta
      301 agtccgccag tcccgcccgt aaaactgacc ggctccgtgg ttgccttttc acaggcataa
      361 gctgttgccc cttcagactg aacagcaatt tcgccattat cacaaagagg ataatcatca
      421 ctaccattga gaagatccaa aggacagcca gaattaaatc ccacctgaat cactgctcca
      481 ggatcatccg gtttccctgg aagagccaga taccaggtag ccgttctgga ctcaagtgaa
      541 gaataagaaa cagaataacc gggatatttt gcatccttca tacgggcaag cgcagaccat
      601 ggtgacgtgc tgtaaacccc tgaaccatta ctgattatac cgcaccagta gttatggtca
      661 gatgccgtca tagctggcgt ggcaaacgtc acatcataac cattagccga taaagccatc
      721 cgcgcaaaag actccgcaac aggcttaccc agtgcataaa gccctactga tgcagccgtc
      781 acccccaaac cataagcaag gtttgcccag gtcatggagc caatggcaac accagaacgg
      841 gctgcataac tcacgacaaa atctgctgcc gtctgagaat acgctttaac gacaaaagca
      901 acagtggcag catcagcacc aagtgaaaga agccgtgttg tcagaatgcc ggataattca
      961 gactgaaacc ccgttgcacg tatctgacca cctttatcca catcagaaaa agccataaca
     1021 ttaaaagaac aggaagaaag aagaaaaaga gacaggattt tattaaaacg aagaggtaaa
     1081 aacataataa acatcctcaa ataataaaaa acattaatac ctcagcgact gaacataaat
     1141 ccgagcacag cagaaaaacc gcataatgca ccgaaaataa aaatcacgac atcaaaaata
     1201 gactgagaca tatatcacca taaaaaaggg gcaaaacatt gccccttcat aaaatcagat
     1261 agatttaatt tgacgccaga cagcacgaac cccgataact gcaagataaa cggtaacagc
     1321 agcagcaccg atagaaagaa ttacagagac aacagaagta aagttaatcg acgtagttaa
     1381 cggcgtaaaa tcaacagacc ccgtagttgt agtagtgcca gccgcaaaaa cctgaccgga
     1441 tgcaaacaat acaggaacag ctaacacttt ggatttaaaa ctaccacaag aagatttaaa
     1501 tttataaaac atatcaaatt cctttaactg ttttaatgat tgcagaaaca cacatgccaa
     1561 taaaaaagaa aaataatggc aataatgctc ctacgctaaa agccccggga ttaaccgaag
     1621 gcacatcaga aaaatccgca caaacaacag atataggctt attattttgt acagaatttt
     1681 cagaaaaatt actgttaccg ttaccagatg gctgtggtga agatgacgaa gaaaaaagac
     1741 cacagttata agaaccatca ggctgaacct gacaaaccat aaaactcaac cctcagtatt
     1801 acttttcctt acctgatgga gcatcaagag gcaaaccatc aaaccgcttc atcgttccgg
     1861 actgataacg ataccagaca ccggaacgtt cagctgaaac cgcccaggaa ttcacatcaa
     1921 cacagatata aacttccttc ccgtggaggt cagaacagga attgaaatgt ttatattcat
     1981 cttctgtaca tgaaaatttt tcataagcgg tatcccgaat ttttaatttg cggtcctcat
     2041 aaggatacgc cagaacaatg gcatatttag gtttttccct ggcatcaaag taagcacttt
     2101 tagggtcaag ctgc
//
LOCUS       DAZGJT010000247         2102 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZGJT010000247 DAZGJT010000000
VERSION     DAZGJT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.252"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..1025)
                     /locus_tag="ACHKFK_004893"
     CDS             complement(60..1025)
                     /locus_tag="ACHKFK_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HFO9055023.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1892..>2102)
                     /locus_tag="ACHKFK_004894"
     CDS             complement(1892..>2102)
                     /locus_tag="ACHKFK_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055024.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          580 a          552 c          440 g          530 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttacc ccccccaaaa caagcacccc cgaacggctt accccaacct
     1381 tttccacacg cccgatcacc aaaacaggga ataaaaacaa aaccctgtct tacaggtgga
     1441 tggcctcact tcgtgaaacc atccaccccg cataagtttt ttaatcggtg gtgtcgtcaa
     1501 tcactaaatc ggaaccccaa aggggacccc cacgatttag catttgacga gattaactct
     1561 cggagatggc atcatgcttt taacggactt taaaaaacgg tgaacggatg cggattttaa
     1621 tccgtgtctg tgaggcgttg agcaacgagc aaagcgaggc ggtagaaaga gagccagcgg
     1681 caggctcagg acgataatcc ggggaaaatg taccactcgc agagtgttaa catttgatat
     1741 cggattgtct atcctgcatt ggtgtcgagt tggcaaggct gaggataata ctgaggataa
     1801 tgctgaggat aatgcaggat agacagagca ctagagattg catgcaatct cgtacctatc
     1861 acctcagcct gccgctggct cttttccaga actaagaatg cgttcttaaa aatccaaact
     1921 tgataccgtt taaatctatt ttcacaaaaa gagtttttac cctctcgttt gttttctgaa
     1981 tatataaaat taattcatcc tcaaaacctt cgtgcggatg aaaaccaaat gtgccaaaac
     2041 ttaatccatc aataagcaaa ataaggtttt taaacctgtc aggaatcacc gcttcacttc
     2101 tt
//
LOCUS       DAZGJT010000248         2070 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZGJT010000248 DAZGJT010000000
VERSION     DAZGJT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.253"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..946
                     /gene="virK"
                     /locus_tag="ACHKFK_004895"
     CDS             <1..946
                     /gene="virK"
                     /locus_tag="ACHKFK_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HFO9055025.1"
                     /translation="SISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINPVV
                     SIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNLPE
                     KYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTFSV
                     ILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNISEC
                     LAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIA
                     SKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            1011..1955
                     /gene="lpxM"
                     /locus_tag="ACHKFK_004896"
                     /gene_synonym="msbB"
     CDS             1011..1955
                     /gene="lpxM"
                     /locus_tag="ACHKFK_004896"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO9055026.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKNDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGKKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHAREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHLLTIEIRPPMDACIASADNKMI
                     ARQMNKTVEILVGPHPEQYVWVLKLLKTRKSNEADPYP"
BASE COUNT          610 a          399 c          459 g          602 t
ORIGIN      
        1 ttctataagt aacttatcat ttatcggttt ccttaaaagg attatttttt cctcagattc
       61 actaccgggg aagtgggaac acagaaaatt tcggttcatg tacattttgc gatgttctat
      121 aaatccggtt gtcagtattc gatattatta tgaactgcgt tccttgccgt gcattgagga
      181 tattctggca atacacccca cgttgccagc aagaattcat cgcccttatt tgcataaagg
      241 ggggcgcgcc tggacccgtg gacaatatat tcttgagcat tatcgctttg tgcaaaattt
      301 gccagaaaaa tattctaaat ttttgtttcc acaaaaatcg gtatcattag tccaattcat
      361 cgggaaagat ggagagaatt ttgatatcca gtgttctccc agtggttttg accgggaagg
      421 agagttgatg ttgtcattgt tttacaataa aacagttatt gcccggttaa cattttcagt
      481 cattctgact caaaatggac acattgcttt tattggcgga ttacagggag cccctaaaaa
      541 taccggacct gatgttattc ggtgcgcaac ccgggcctgt tatgggattt tcccaaaacg
      601 gattattttt gaagcatttt gtgcgttgat gaaagcttgc aatatctctg agtgtctggc
      661 agtcagtgag cacagtcatg ttttccggca attgagatac tggtatcaga aacgcaaaac
      721 ctttgttgcg gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg
      781 gtacagatta ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg
      841 ctctgagtac cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc
      901 tttggatgca tatcctgtac actcagaaca tcagaattta aattaaggtt tgatgtatcc
      961 tctgaataaa acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat
     1021 ataaatctga gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta
     1081 catggttcct tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc
     1141 ctgtcttggc aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg
     1201 cgacgattaa tttatcgctt tgtttcccag aaaagaatga tacagaacgg gaaataattg
     1261 tcgacaaaat gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc
     1321 gtggtccgga aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa
     1381 ttcgacacaa taatagaaat gtgatttttc tggttcccca tggctggagc gtggatattc
     1441 ctgcaatgtt gctggcagct cagggaaaaa aaatggccgc catgtttcat cagcaacgaa
     1501 atccagtgat tgattatgtc tggaattcag tacggcgtaa attcgggggg cgcttacatg
     1561 cccgggagga tgggataaaa ccatttattc agtcagtacg ccagggatac tggggatatt
     1621 acctcccaga tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct
     1681 ataaagcgac attaccaatc attggacgtc tgatgaacat cagtcaggct atgattatac
     1741 cacttttccc ggtttatgat gaaaaaaaac atctcctgac tattgaaatt cggccaccaa
     1801 tggatgcttg cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag
     1861 tggaaatttt ggtggggcca catccggaac agtatgtctg ggttttaaaa ttgttaaaaa
     1921 cacgcaaatc aaacgaagcg gacccgtacc cttgaaatta taaagtataa gtatttccac
     1981 tagttgtttg taagcgtaca gcctgaaccg tctggtcaga atctgacgaa ttagacaaag
     2041 tggtgtccac caaataagta gtgggaacca
//
LOCUS       DAZGJT010000249         2017 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZGJT010000249 DAZGJT010000000
VERSION     DAZGJT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.254"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1388)
                     /gene="tssI"
                     /locus_tag="ACHKFK_004897"
     CDS             complement(<1..1388)
                     /gene="tssI"
                     /locus_tag="ACHKFK_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO9055027.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVSFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKNATLTIWQGVIPLRYVTGVVAGFGMQENNGWQMRYHLRIEPPLWRC
                     GLRQNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPAREFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGEFPFNPDTSAGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGESLNGQLEQYEIFDYPGRYKDEQHGKDFTLY
                     RIESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVIQSILSGDQPQALHG
                     SQGRGTTLGNQLEVIPADRTWRPRVQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVK
                     FHWDRYHGMTEESSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPLVMGRT
                     YHEDNRSPGDLPG"
     gene            complement(1407..1691)
                     /locus_tag="ACHKFK_004898"
     CDS             complement(1407..1691)
                     /locus_tag="ACHKFK_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system PAAR protein"
                     /protein_id="HFO9055028.1"
                     /translation="MPLAAKLTDKGTQHDGYYETVITAGSSTVFIDGLPAARQGDPLT
                     PHAKPKHPPHPRKIARGSSTVFIDGLPAARTGDAIDCGGVVIGGGTVNIG"
BASE COUNT          454 a          643 c          464 g          456 t
ORIGIN      
        1 cccggcaggt cccccggcga acggttgtcc tcatggtacg tccggcccat gaccagcggc
       61 tggtccgggt ccccgttcag aaaatcaaca atcacctcct ggcccacccg cggtatcgcc
      121 aggttaccga accccggccc cgcccacgcc tgcgacacac gcacccagca cgaactctcc
      181 tccgtcatcc cgtgataacg gtcccagtga aacttcaccc gtacccgacc atgttcgtcg
      241 cagaagattt cctctcctgc gggaccggtg acaatggcgc tctgtggccc gtccaccttc
      301 ggtttgctct gcacccgtgg acgccaggtc cggtccgccg gtatcacctc taactgattt
      361 cccagcgtgg ttcctctccc ctggctgcca tgaagcgcct gcggctggtc ccctgacaga
      421 atactctgta tcacctgcca ttcacggttc agcatcttct gcggatgccc cgtcagcgta
      481 aaccgcgttc ccggccacag cttcggcgaa ttactctgcc ctgtggcctt ttccgcatca
      541 ctgcgcaggc tctctatccg gtacagcgtg aaatccttgc cgtgctgttc atccttgtac
      601 cttcccggat aatcaaatat ctcatactgt tcaagctgtc cgttcaggct ctcaccctgc
      661 tgctcataca tccccggcca gtccggcacc ttaaacgtgt aatcctggct ctgcaccgat
      721 gacgggcgga catgcgcctc ataacggaac atactgacac actccgtctc cgctcctgcc
      781 gatgtgtccg ggttaaacgg aaactccccc gcctgtgaca gccccgccac atcgtcgcac
      841 agcgtcagct tctgctccgg actgtccgcc gcaaaccgct caaagaagaa aatcccctcc
      901 tccgcccaca gccgcgccag aaacgccagg tccgactccc cgtactgcac acagaactcc
      961 cgcgccggat ggtcctcata aaacagcggc gtccactccg tgacgccgtt ctcgttcagt
     1021 aacgtggccg atatcgtccg gatatcctgc tgctggaaga tgcggaagtt ctgccgcagc
     1081 ccgcaccgcc ataacggcgg ctcaatacgc aggtgataac gcatctgcca gccgttgttc
     1141 tcctgcatac caaaccctgc caccacgcct gtgacataac gcagcgggat gacaccctgc
     1201 cagatagtca gcgttgcgtt cttctccagc agcatctccg ccgtctgcat aaaagaatcg
     1261 ctggcgacat ccacactcat cacaaacgga taagactggt tctggattaa ccggaagctc
     1321 accactgcaa aggtgtccgg ctcctggccg tcaacctcca gcgtaaagcg aagacctttt
     1381 aaggacataa atacctctca gacatatcag cctatattta ccgtgccgcc accaataacc
     1441 acgccgccgc agtcaatagc atcaccggtt ctggcggcgg gcaggccgtc aataaagacg
     1501 gttgacgagc ctctggcgat ttttcgggga tgaggtggat gcttcggttt agcatgcggc
     1561 gtcagcggat ctccctggcg ggctgcgggg agaccatcga taaaaacggt ggatgacccg
     1621 gcggtgatga cggtctcgta ataaccgtcg tgctgggtgc ctttgtcggt aagtttggct
     1681 gctaaaggca taattcctcc tgttcagaat ttctacagca gcaggtttca aaaatccgga
     1741 gaaatattca acgaaatatt aatcacttca ccggatattc tgatgcgtac cttcactggt
     1801 ctatttaagt aatacaaatt gttggcgtgt attaagtttc attttgttct ccttaattat
     1861 gtaaggagta tttgatcatg tataagcaat gaaaaacagc ttcaggaaat aaggagtatc
     1921 tcaattttta aacataaaat gaaaatcatt cagtacaaaa aagcagcatt acgtaatctt
     1981 tccctgatta aatacgctac cgcccagccc tgataac
//
LOCUS       DAZGJT010000250         2000 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZGJT010000250 DAZGJT010000000
VERSION     DAZGJT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.255"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..790)
                     /locus_tag="ACHKFK_004899"
     CDS             complement(<1..790)
                     /locus_tag="ACHKFK_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO9055029.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTI
                     AQAKELFALAKSKGLIVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMP"
     gene            1124..1612
                     /gene="yhhY"
                     /locus_tag="ACHKFK_004900"
     CDS             1124..1612
                     /gene="yhhY"
                     /locus_tag="ACHKFK_004900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO9055030.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYYNTLQVPHPSEQMWLER
                     LTARPGIKQLVACIDGIVVGHLTIDVQQRPRRSHVADFGICVDSRWKNHGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            1848..>2000
                     /locus_tag="ACHKFK_004901"
     CDS             1848..>2000
                     /locus_tag="ACHKFK_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFO9055031.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTTESIGNRWQSGHEDEIFTFSL
                     LNSITNT"
BASE COUNT          546 a          449 c          482 g          523 t
ORIGIN      
        1 ccggcataat attagccttc aggctggttt cctgctggtc gataccgtat ttaataaacg
       61 aacctttctt accgtgaacg ataaatttcg gataatcgat tttcaccaga tggctggttt
      121 tgacgatggc ttttagatcg ccataaaaca gctgcgcttc aaaggtgtcg tccggattgg
      181 ctttattacg caggctgcgg atgtcataag cgacgtgatc cgggcgaccg aacagagaaa
      241 taatctggtc catcgtatgc acaccaaggc catagaacgc gccatcctgc ggcagccccg
      301 gtttggtttc tgccaccggg cggtaatagt caaaatggct ttccacttcg acaatctctc
      361 ccagcttgcc gctttcaatc gcttttttcg ctgtcaggaa gcaggaatca aaacgacgat
      421 tctgatacgg cgtaacgatc agccctttgc ttttcgccag tgcaaacagc tctttcgcct
      481 gcgcaattgt cggagtgaac ggtttttcga ccagcacatt tttcccggct tccagcgcgc
      541 gcttcgcgta ctcgaagtgg ctgtccgcgt gggtgcagac gacaaccagc ttaacatcgg
      601 gatcgtttag cacttcgtcg agatcgctgg tgaaatggat atgggaataa atgggggcct
      661 gttcttccgg cttcgcatgg cggcgaaaaa tatgcgcgac atgccagcta tccttgcggt
      721 taagtacata cggcagatgg taacgggtgg tgcttttgcc gaagccaata aaggcgcagt
      781 taatgaccat gatttcgtcc tttttaaggt ggttatttac accttagcgc aaagcggacg
      841 tggttcctac tggagtgcgc ataacgaaca caagcacttc ccgaggataa attgagaacg
      901 aaaggtcaaa aaaaaagcca gcacccggct ggctaagtaa tactggaagc aatgtgagca
      961 atgtcgtgct ttcaggttct ccgcgagggt cttcctgatc gcgagacaat aataatcatt
     1021 ctcattcgca cttgtccaac actttttgca aaaaaatgca tttgactcgc atttgaaagt
     1081 caatgatgtt gaaagggaca tttaccccaa agaggacaaa ggaatgagtg agatagtaat
     1141 acgccacgca gaaacacgcg attacgaggc catcaggcag attcacgccc agccggaggt
     1201 gtattacaac acgctacagg tgcctcatcc ttcagaacaa atgtggctgg aacgactcac
     1261 cgctcgccct ggcatcaagc agttagtcgc ctgtattgat ggaatcgtcg taggccatct
     1321 caccattgac gtgcaacaac gcccacgccg cagtcatgtt gccgattttg gtatctgtgt
     1381 cgactcccgt tggaagaacc acggcgtcgc cagcgccctg atgcgagaga tgattgaaat
     1441 gtgcgacaac tggttgcggg tagatcgtat tgagctaacg gtgttcgtcg ataacgcgcc
     1501 agcaattaag gtctataaaa aatttggctt tgagattgaa gggactggca agaagtacgc
     1561 gctgcgtaat ggtgaatatg tcgatgcgta ttatatggcg cgggtgaagt aagatagtgc
     1621 cctttttctg agatggaaaa agggtgtcat tcaaaatcgg cataccttcc tttaatgtat
     1681 ttattttccc aataaacata tgagatttac ctcataatta cttcctaaag tgtaatattt
     1741 tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat taacaagcat
     1801 aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg agtaatattg
     1861 tttatctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt gggactactg
     1921 aatctatagg taatcgctgg cagagcgggc atgaggatga aatatttaca ttctcactct
     1981 taaatagtat tactaatacg
//
LOCUS       DAZGJT010000251         1954 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZGJT010000251 DAZGJT010000000
VERSION     DAZGJT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.256"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..895
                     /locus_tag="ACHKFK_004902"
     CDS             53..895
                     /locus_tag="ACHKFK_004902"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO9055032.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKLTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPDD"
     gene            898..>1954
                     /gene="capU"
                     /locus_tag="ACHKFK_004903"
     CDS             898..>1954
                     /gene="capU"
                     /locus_tag="ACHKFK_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HFO9055033.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKFGIDITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTW
                     KHPFRIIRQKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDF
                     QELYVDSRNSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVVATQIGGIPEVIQNNQTGTLLPAGNKHAWMCALNNFFNDPGRFYQM
                     ARLAKQDIEERFDINKTA"
BASE COUNT          565 a          357 c          445 g          587 t
ORIGIN      
        1 actttattag gctttccttt tatctctact tactgggtgc cagaaacttt aaattttaaa
       61 cggagggggc attttgttta aagcaaatca tgttccggta ttaatgtatc atcatgtcag
      121 ccattgccca ggactggtta cactttctcc cgagacattc cggaaacaaa tgaaatggtt
      181 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggtgg
      241 aaaacttccc cgtaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
      301 tcaggtatat cctttattaa acgagtttaa tctcaaggca catatttttt taattaccag
      361 ttttattgga aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
      421 cgaacatcaa atagcaaccg ggaatgctga taatgttatg cttcgctggt ctgaagtgaa
      481 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acacatactc atacacgttg
      541 ggataaaaag ctcacttcac gagaagaaca gtgtaaacat cttcggcagg acttattgag
      601 cgggagagaa tatttaaaaa aaatgacagg aaaatgcagt aaacatttgt gttggccgga
      661 aggttattat aacaaggatt atattcagat cgctgaagaa cttggattcc attatttata
      721 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
      781 caccaaggaa cgggaaaatt gcgtatggct gaaaagacga cttttttatt atacaacacc
      841 gtttttttca tcgttgctgg cccttcataa ggggccaaga ttaccggatg actaataatg
      901 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
      961 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggagaacagc
     1021 aaaattgctt ttgaagccag taagtttgga attgatatca cattcgcgtt atttcgaaac
     1081 agtcttcaca ttcctactgt atggagatta ctcggaatag ttcatagttt tcagcccgat
     1141 gcaatcgttt gtcacagtgg acatgatagt aatattgttg gtttggtacg gttttttacc
     1201 tggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaattt
     1261 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaaatat gaagacacat
     1321 ctggagcagg aaggatgtcg aactcgggtt actgttgtgc ctcctggctt tgatttccag
     1381 gaattatacg ttgattcccg aaacagtttg cctccgagtg ttctttcctg gctggcatcc
     1441 cgaagaggat gccccgtaat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     1501 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     1561 gtggggtctg gttcacctga actgcgggaa catttacagt atcagattga cagtatgggc
     1621 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     1681 gccagtctgg tggttctgcc ttcagaaaac gaatcttttg gtatggtgct ggcagaagca
     1741 tcggcatttt ctgtgcctgt agtggccact cagattggtg gaatccctga ggttattcag
     1801 aacaaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     1861 aataattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgcctggc aaaacaggat
     1921 atagaagagc ggtttgatat taataaaact gcgt
//
LOCUS       DAZGJT010000252         1942 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZGJT010000252 DAZGJT010000000
VERSION     DAZGJT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.257"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..1475
                     /gene="gadC"
                     /locus_tag="ACHKFK_004904"
     CDS             15..1475
                     /gene="gadC"
                     /locus_tag="ACHKFK_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glutamine/gamma-aminobutyrate
                     antiporter GadC"
                     /protein_id="HFO9055034.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSILSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFAGAIY
                     LRHSQPDKKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            1509..>1942
                     /locus_tag="ACHKFK_004905"
     CDS             1509..>1942
                     /locus_tag="ACHKFK_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9055035.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNTNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNPTINI"
BASE COUNT          531 a          354 c          402 g          655 t
ORIGIN      
        1 tcgagagtat aaaaatgtcg aatgctaata tcgcacccaa aagtaagaaa atgacagtgg
       61 ggacactcgc tatcatgaat ataactgctg tagtaagtct acgatttata ccttctgaag
      121 cagaatacgg cttaggtgcc attttttact atacctttgc tgcaattatg tttttagtac
      181 ctgttgcctt tgttgcggct gagctagcta caacatatcc acaaaaaggg ggggcatttc
      241 gatggatagg agaagcattt ggggctcgct ggggttttgt aggaatgctt atgacctggc
      301 tacaagtcat cccctacttt ccaacagtac tgacatttgg tgcagtctcg gttgcattta
      361 ttgatgttga tatgggggtg gcagaatcaa ttgccagtaa taaatggtat attttatttt
      421 tcgtactatt tgtttactgg ggagctattt gtgttgcatt acgtggcgtt ggtattttct
      481 cgcaggtatc taagtggtgc ggtattatag ggacaattat tccagctatt atccttgtta
      541 ttcttggttt ctcctatctg tggttcagcg gtaaaccagc ggtgatccca ttaggttggg
      601 gtgatctttt ccctaatata gctaacttcc agaatattgt tcttgccgcc agcatatttc
      661 ttgcttatgc agggatggaa atgaatgcgg tccatgttaa cgatttagat aatccaacta
      721 aaaaataccc aattgcaatt accatcgctt ctataggtac tattgcaata ttcctgctca
      781 gtactttagg ggtcgccttt attattccta cagataaaat tagtctaact caaagtttgt
      841 tggttgccta cgatatgctg ttcgaatggg ctggagtgcc atggcttggc tctgtgatgg
      901 catttatgct agcaattggc gtactgggtg gggtggttac ttggattgca gggcctaata
      961 caggtgtatt agcgatcgcg aaagctggtt atttacctaa gtttttccaa aaaaccaatc
     1021 gtcatggaat ggggcaccat ctgatgtttg tacaaggtat aattgtctca attctttctg
     1081 ttacttttgt gataatgcct tcagttcaag cagcttttca gattttatca cagttaacag
     1141 ttatgctcta tttagttatg tatatgctca tgttcgctgg tgctatctat ctacgacatt
     1201 ctcaacctga taaaaaacgc ccatatcgag cccctgcttt attcttctgg gcaacacttg
     1261 gattcttagg gtcttttctg gcttttgtat taagttttat tccgcccgag caaatcccaa
     1321 ctggtagtcc aaccagctat gttctttatt tgttagtatt agttgcccta tttttattaa
     1381 ttccaatatt aatttacgcc ttccgaaaat ctgactggaa agatccaaac tctgatttcg
     1441 aaccattttc ttgggaaaaa agaactagta attaaatttt caataagcta caaaagatga
     1501 ggttaaatat gtttagtgct gaatctccaa ttggtgtaat agactcaggt gtaggtggat
     1561 tgacatcggt aaaggaaata attaatttgc ttcctggcga agatattatc tattgtggag
     1621 ataatacgaa tgcaccttat ggcaaccgtt ctgccgatga tatcatcagt ttgactaaaa
     1681 aaatgctgac ttttttacag tcaaggaatg tcaaactagt tgctgttgca tgtaatacta
     1741 tatcctcaac tttggaatcc gaagaataca gcggatatgc taagagcttt ccgtttccta
     1801 tcttatccat cattgaacct gcagtagaag atgtgattcg ccagcagtat aaaaatgtgg
     1861 gtattatcgc taccgagttt actattaaaa cggggtgtca caaagagcta ataaaaaaat
     1921 tgaacccaac aataaatata tt
//
LOCUS       DAZGJT010000253         1927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZGJT010000253 DAZGJT010000000
VERSION     DAZGJT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.258"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..520
                     /locus_tag="ACHKFK_004906"
     CDS             263..520
                     /locus_tag="ACHKFK_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFO9055036.1"
                     /translation="MSQTVNAETSSSGKKRPYRRGNPVPAKERQKASLARRSATHKAF
                     HAVIQLRLKEKLSELADEDGITQAQMLEWLIESEVKRRKSL"
     gene            754..828
                     /gene="tap"
                     /locus_tag="ACHKFK_004907"
     CDS             754..828
                     /gene="tap"
                     /locus_tag="ACHKFK_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HFO9055037.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            821..1678
                     /gene="repA"
                     /locus_tag="ACHKFK_004908"
     CDS             821..1678
                     /gene="repA"
                     /locus_tag="ACHKFK_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO9055038.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESGAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTPALFAALDI
                     SEEAVASARRSRVEWENRQRKKQGLDTLGMDELIAKAWRFVRERFCSYQTELKSRGIK
                     RARARRDANRERQDIVTLVKRQLTREISEGRFSASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
BASE COUNT          495 a          491 c          494 g          447 t
ORIGIN      
        1 gcaggtatct ttgcctttct gattcagata tctgcccgtg cttaatatct caagtgctgc
       61 cggcttctcc tggcaggcag cacagcagtt aagatttctc cttgttttta gctatttcgc
      121 aacaaaaatc acattaaatc tccacaatcc gagatgattt gtgcgccaac acagatcatg
      181 atcacaattc tcaagtcgct gatttcaaaa aactgtagta tcctctgcga aacgatccct
      241 gtctgagaag agaggaggca agatgtcgca gacagtaaat gcagagactt cctcatcggg
      301 aaaaaagcga ccttacagaa ggggtaatcc tgttccggcg aaagaacgac aaaaagcgtc
      361 tctcgcaaga aggagtgcca ctcataaagc gtttcatgcc gtcattcagc ttcgcctgaa
      421 agaaaagtta agtgagcttg ctgatgaaga cgggatcact caggcacaga tgcttgagtg
      481 gctgatagag tcagaggtga aacgcagaaa atctttgtga atatttgcgt ttcttgctgt
      541 atcagtactg agatgttaga ttgctgatcg tttaaggaat ttagtggctg gccacgccgt
      601 aaggtggcag ggaactggtt ctgatgtgga tttacaggag ccagaaaagc aaaaaccccg
      661 ataatcttct ttaactttgg cgagtcagaa agattaccgg ggcccactta aaccgtatag
      721 ccaacaattc agctatgcgg ggagtatagt tatatgcccg gaaaagttca agacttcttt
      781 ctgtgctcgc tccttctgcg cattgtaagt gcaggatggt gtgactgatc ttcaacaaac
      841 gtattaccgc caggtaaaga acccgaatcc ggtatttaca ccccgtgaag gtgccggaac
      901 gctgaagttt tgcgaaaaac tgatggaaaa ggcggtgggc ttcacctccc gttttgattt
      961 cgccattcat gtggcgcatg cccgttcgcg tggcctgcgt cgacgcatgc caccggtgct
     1021 gcgtcgacgg gctattgatg cgctgctgca ggggctgtgt ttccactatg acccgctggc
     1081 caaccgcgtc cagtgttcca tcaccacact ggccattgag tgcggactgg cgacagagtc
     1141 cggtgcagga aaactctcca tcacccgagc cacccgggcc ctgacgttcc tgtcagagct
     1201 gggactgatt acctaccaga cggaatatga cccgcttatc gggtgctaca ttccgaccga
     1261 tatcacgttc acaccggcgc tgtttgccgc tctcgatatc tcagaagagg cggtggcttc
     1321 cgcacgccgc agccgtgtgg aatgggaaaa cagacagcgc aaaaagcagg ggctggatac
     1381 cctgggtatg gatgagctga tagcgaaagc ctggcgtttt gtgcgtgagc gtttctgcag
     1441 ttaccagaca gagcttaagt cccgtggaat aaaacgtgcc cgtgcgcgtc gtgatgcgaa
     1501 cagggaacgt caggatatcg tcaccctggt gaaacggcag ctgacgcgtg aaatctcgga
     1561 agggcgcttc tctgccagtc gtgaggcggt aaaacgtgaa gtggagcgtc gtgtgaagga
     1621 gcgcatgatt ctgtcacgta accgcaatta cagccggctg gccaccgctt ccccctgaaa
     1681 gtgacctcct cagaataatc cggcctgcgc cggaggaatc cgcacgtctg aagcccgcca
     1741 gtacagaaaa aaacagcacc acatacaaaa aacaacctca tcatccactt tcagatgcat
     1801 ccggttctct ctgtttttga tacaaaacac gcctcacaga cggggaattt tgcttatcca
     1861 cattaaactg caagggactt ccccataagg ttacaaccgt tcatgtcata aagcgccatc
     1921 cgccagc
//
LOCUS       DAZGJT010000254         1912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZGJT010000254 DAZGJT010000000
VERSION     DAZGJT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.259"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..1088
                     /gene="istA"
                     /locus_tag="ACHKFK_004909"
     CDS             66..1088
                     /gene="istA"
                     /locus_tag="ACHKFK_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HFO9055039.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1085..1867
                     /gene="istB"
                     /locus_tag="ACHKFK_004910"
     CDS             1085..1867
                     /gene="istB"
                     /locus_tag="ACHKFK_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HFO9055040.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          500 a          495 c          491 g          426 t
ORIGIN      
        1 gatccacacc caacgcctga aatcagatcc agggggtaat ctgctctcct gattcaggag
       61 agcttatggt cacttttgag acagttatgg aaattaaaat cctgcacaag cagggaatga
      121 gtagccgggc gattgccaga gaactgggga tctcccgcaa taccgttaaa cgttatttgc
      181 aggcaaaatc tgagccgcca aaatatacgc cgcgacctgc tgttgcttca ctcctggatg
      241 aataccggga ttatattcgt caacgcatcg ccgatgctca tccttacaaa atcccggcaa
      301 cggtaatcgc tcgcgagatc agagaccagg gatatcgtgg cggaatgacc attctcaggg
      361 cattcattcg ttctctctcg gttcctcagg agcaggagcc tgccgttcgg ttcgaaactg
      421 aacccggacg acagatgcag gttgactggg gcactatgcg taatggtcgc tcaccgcttc
      481 acgtgttcgt tgctgttctc ggatacagcc gaatgctgta catcgaattc actgacaata
      541 tgcgttatga cacgctggag acctgccatc gtaatgcgtt ccgcttcttt ggtggtgtgc
      601 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
      661 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      721 cccgactgtg tcgccccttc agggcacaga ctaaaggtaa ggtggaacgg atggtgcagt
      781 acacccgtaa cagtttttac atcccactaa tgactcgcct gcgcccgatg gggatcactg
      841 tcgatgttga aacagccaac cgccacggtc tgcgctggct gcacgatgtc gctaaccaac
      901 gaaagcatga aacaatccag gcacgtccct gcgatcgctg gctcgaagag cagcagtcca
      961 tgctggcact gcctccggag aaaaaagagt atgacgtgca tcttgatgaa aatctggtga
     1021 acttcgacaa acaccccctg catcatccac tctccatcta cgactcattc tgcagaggag
     1081 tggcgtgatg atggaactgc aacatcaacg actgatggtg ctcgccgggc agttgcaact
     1141 ggaaagcctt ataagcgcag cgcctgcgct gtcacaacag gcagtagacc aggaatggag
     1201 ttatatggac ttcctggagc atctgcttca tgaagaaaaa ctggcacgtc atcaacgtaa
     1261 acaggcgatg tatacccgaa tggcagcctt cccggcggtg aaaacgttcg aagagtatga
     1321 cttcacattc gccaccggag caccgcagaa gcaactccag tcgttacgct cactcagctt
     1381 catagaacgt aatgaaaata tcgtattact ggggccatca ggtgtgggga aaacccatct
     1441 ggcaatagcg atgggctatg aagcagtccg tgcaggtatc aaagttcgct tcacaacagc
     1501 agcagatctg ttacttcagt tatctacggc acaacgtcag ggccgttata aaacgacgct
     1561 tcagcgtgga gtaatggccc cccgcctgct catcattgat gaaataggct atctgccgtt
     1621 cagtcaggaa gaagcaaagc tgttcttcca ggtcatcgct aaacgttacg aaaagagcgc
     1681 aatgatcctg acatccaatc tgccgttcgg gcagtgggat caaacgttcg ccggtgatgc
     1741 agcactgacc tcagcgatgc tggaccgtat cttacaccac tcacatgtcg ttcaaatcaa
     1801 aggagaaagc tatcgactca gacagaaacg aaaggccggg gttatagcag aagctaatcc
     1861 tgagtaaaac ggtggatcaa tattgggccg ttggtggaga tataagtgga tc
//
LOCUS       DAZGJT010000255         1894 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZGJT010000255 DAZGJT010000000
VERSION     DAZGJT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.260"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..77
                     /locus_tag="ACHKFK_004911"
     tRNA            2..77
                     /locus_tag="ACHKFK_004911"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Thr,seq:ggt)
     gene            117..232
                     /gene="rrf"
                     /locus_tag="ACHKFK_004912"
     rRNA            117..232
                     /gene="rrf"
                     /locus_tag="ACHKFK_004912"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(295..1053)
                     /locus_tag="ACHKFK_004913"
     CDS             complement(295..1053)
                     /locus_tag="ACHKFK_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO9055041.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLIVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAQKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPNEFFAHPKSERTRAFLSQVIH"
     gene            complement(1061..>1894)
                     /locus_tag="ACHKFK_004914"
     CDS             complement(1061..>1894)
                     /locus_tag="ACHKFK_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO9055042.1"
                     /translation="LYPHDQRWRINLALLIGLVSIAPMFWKILPHRGRYIAVWAVIYP
                     LVVWWLMYGGFLGLDRVETRQWGGLTLTLIIASVGIAGALPWGILLALGRRSHMPIVR
                     ILSVIFIEFWRGVPLITVLFMSSVMLPLFMAEGTSIDKLIRALVGVILFQSAYVAEVV
                     RGGLQALPKGQYEAAESLALGYWKTQGLVILPQALKLVIPGLVNTIIALFKDTSLVII
                     IGLFDLFSSVQQATVDPAWLGMSTEGYVFAALIYWIFCFSMSRYSQHLEKRFNTGRTP
                     H"
BASE COUNT          513 a          516 c          430 g          435 t
ORIGIN      
        1 tgctgatatg gctcagttgg tagagcgcac ccttggtaag ggtgaggtcc ccagttcgac
       61 tctgggtatc agcaccactt tttaggttaa agttcggcag attagaaaag aatttgtctg
      121 gcggcagtag cgcggtggtc ccacctgacc ccatgccgaa ctcagaagtg aaacgccgta
      181 gcgccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag acatcaaatc
      241 aagcgaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat tcaattaatg
      301 gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgcaa aaaattcatt
      361 aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt cagcgacggt
      421 tcgtgcaaac cccatctcat gtgttacaca caacattgtc atacccgact gcgccagccc
      481 aatcatcgta tccagcacct ctttcaccat ctcaggatcg agcgccgacg ttggctcatc
      541 aaacaacata attttcggct tcatacacag cgaacgtgct atggcaacac gttgctgctg
      601 accaccagaa atctgtccgg gaaacttctg ggcatgttcg gcaattctca cccgctcaag
      661 gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc aaatcggtgc
      721 cagggtacag ttctgcaaca cggtcagatg agggaagaga ttgaaatgct gaaagaccat
      781 tcccacttcc tgcctgacgc gctcaatatt gcggatatct tcattaagtt cgatgccatc
      841 taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa tggttgtcga
      901 tttaccggaa cctgaagggc cacacagaac gatccgttcg cccggttgca cgattaaatt
      961 tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca gcgtaatcat
     1021 cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt gtacgcccgg
     1081 tgttaaaacg tttttccaga tgttggctat aacgtgacat gctgaaacag aagatccagt
     1141 agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggcg ggatcaacgg
     1201 ttgcctgctg aacgctactg aaaagatcga acaacccgat gatgatcacc agactggtat
     1261 ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc aacgcctgtg
     1321 gcagaataac cagcccctga gttttccagt aacccaatgc cagcgactct gccgcttcat
     1381 attgcccttt aggcagcgcc tgcaatccgc ctcgcacgac ttccgcaaca tatgctgact
     1441 gaaataggat cacgccaacc agcgcgcgga tcagtttgtc gatactggtg ccttctgcca
     1501 taaacaacgg cagcatgacc gaagacataa acaatacggt aatcagcggt acgccgcgcc
     1561 agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag cggcgaccta
     1621 acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aactgatgca ataattaaag
     1681 ttagcgtcaa cccgccccat tgccgggttt caacccgatc aagaccaaga aacccgccat
     1741 acatcagcca ccagacaacc agtgggtaaa tcaccgccca gaccgcaata tagcgaccgc
     1801 gatgcggaag tattttccag aacattggtg cgatagacac aaggccaatc agtaatgcca
     1861 ggttaattcg ccagcgttgg tcgtgtgggt aaag
//
LOCUS       DAZGJT010000256         1863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZGJT010000256 DAZGJT010000000
VERSION     DAZGJT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.262"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(243..488)
                     /locus_tag="ACHKFK_004915"
                     /pseudo
     CDS             complement(243..488)
                     /locus_tag="ACHKFK_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
     gene            885..1277
                     /locus_tag="ACHKFK_004916"
     CDS             885..1277
                     /locus_tag="ACHKFK_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055043.1"
                     /translation="MELTADLLKSLNNIRQNATIYVHAYHYTDDEKCVMAPRSSLPSL
                     ELTVLQSEKIIDLLLSGYGYIRHDGYVQGYGEKTVATFTIQEGKHHHFRTNLALNKTG
                     NIADNALNHWLHRIRKTDIIVLSVVLTE"
     gene            1337..1618
                     /locus_tag="ACHKFK_004917"
     CDS             1337..1618
                     /locus_tag="ACHKFK_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055044.1"
                     /translation="MKTNKKDTKWYIFYRENSGEDILLEMSSFKECLSTSKELMTPSN
                     YMICIERNGERIKRWDREIIAGSNKWINCPPDNFEILGELITINRIIKK"
     gene            complement(1608..>1863)
                     /locus_tag="ACHKFK_004918"
     CDS             complement(1608..>1863)
                     /locus_tag="ACHKFK_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055045.1"
                     /translation="ETMNSFSEVEKFKGLVFTLDNECEIDKWVSLLAHESRFVKGILQ
                     KIVGKCPGTAMTYKHSPAKNEPVACYSALLNALSKVGVTF"
BASE COUNT          585 a          413 c          378 g          487 t
ORIGIN      
        1 tttcaggtga taaaattcac gacctgataa aaaaactggc ccagtaaaca cggagagtta
       61 tttacaataa caactctgcc atcactgact tattaaaaaa gcgccacaaa atgtggcgca
      121 ttgtttcatt acactatttt tactactggc cccggggacc cgactgatgc tgatgccatg
      181 gcgcgttcgc ctggctgtgt gtccctgatc agacagagtt gtggctgctc atgctccgcg
      241 acctcccatg cctgactggc tacagttgtt gagccggcac cgtgactgac cagccctgga
      301 taaggcggat atgtctccgt tcttaccggt ttcagtatcc ccgcacagcc tgcaagaaac
      361 agcagtatgc tctgctggat cgcatgacgg taccgggatg catcattccc ttcaatccac
      421 tgaatcagta cgtcaagaca gactgactta ccggtgattt caaaacgatt tctgcaccat
      481 tcagccataa tcaatgctcc tctggtaaat gaagagggca ctgtcccgtg aggaacaatg
      541 ccctaacggg gtgaatggcg gtcgggttac accctccgcc agcggttcag attaagcaac
      601 cggttatcag gaataacgac ttgaggtagc ctgaaaaaag aaactgcaat ccagccagcc
      661 cataaactgc gacacgtctt acagaagatg gatgtgtctt taccatcaat attctcctgc
      721 ttcaacaaat gaagcaggga atatcgtcag aaagcaacag gtcgtgaaca aataactctt
      781 tttgtattct gtaaatttct cagaagagaa aaagaattaa ttgtcgccaa cgctttcaac
      841 aggtcgcatc atcaacagat gacaacaacc ggaaggacac cgcgatggag ttgactgcag
      901 acctgctgaa aagccttaac aatataagac aaaatgccac catatatgtt cacgcatacc
      961 actatactga tgatgaaaaa tgcgttatgg caccacgctc atccctgccc tctctggagc
     1021 taacagttct gcagtcagaa aaaattatcg atctgcttct gagtggttac ggttatatca
     1081 ggcatgacgg ctatgttcag ggatatggag aaaaaactgt ggcaactttc acaatacagg
     1141 agggtaagca ccatcatttc aggacaaatc tggcactgaa taagacgggc aatatagccg
     1201 ataatgcgtt gaatcattgg ctacatcgta tacgtaaaac agacatcatt gttctgtctg
     1261 ttgttcttac agaataaatt cagttattta ctggaaattt ataaggtctt tatagttatc
     1321 cgccgagaat acccgtatga aaactaataa gaaagacaca aaatggtaca ttttttaccg
     1381 tgaaaattca ggcgaggata ttttgcttga aatgtcatca tttaaggaat gtctttcgac
     1441 atccaaagaa ttaatgaccc catcaaatta catgatatgc atagaaagaa atggcgaaag
     1501 aataaaacgt tgggacaggg aaatcatagc aggctctaac aaatggatta actgtccacc
     1561 tgataacttc gaaatattag gtgagctaat aaccattaat agaattatca aaaagtaact
     1621 ccgactttag acaatgcatt aagcaatgcg ctataacaag caacgggttc attttttgcg
     1681 ggagaatgct tgtaagtcat cgctgtgccc gggcattttc ccactatctt ttgtaaaata
     1741 cctttaacaa atcttgattc gtgagctaac aaagataccc atttatcgat ttcacattcg
     1801 ttatcaagtg taaaaaccag acctttaaat ttttccactt cagagaacga attcatggtt
     1861 tct
//
LOCUS       DAZGJT010000257         1840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZGJT010000257 DAZGJT010000000
VERSION     DAZGJT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.263"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            230..>1840
                     /gene="tssI"
                     /locus_tag="ACHKFK_004919"
     CDS             230..>1840
                     /gene="tssI"
                     /locus_tag="ACHKFK_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO9055046.1"
                     /translation="MSLKGLRFTLEVDGQEPDTFAVVNFRLIQNQSYPFVMSVDVASD
                     SFMQTAEMLLEKKATLTIWQGVIPQRYVTGVVAGFGMQENNGWQMRYHLCIEPPLWRC
                     GLRQNFRIFQQQDIRTISATLLNENGVTEWTPLFYEDHPARKFCVQYGESDLAFLARL
                     WAEEGIFFFERFAADSPEQKLTLCDDVAGLSQAGEFPFNPDASTGAETECVSMFRYEA
                     HVRPSSVQSQDYTFKVPDWPGMYEQQGESLNGQLEQYEIFDYPGRFKDEQHGKDFTLY
                     QMESLRSDAEKATGQSNSPKLWPGTRFTLTGHPQKMLNREWQVVQSILSGDQPQALHG
                     SQGRGTTLGNQLEVIPADRTWRPRQQSKPKVDGPQSAIVTGPAGEEIFCDEHGRVRVK
                     FHWDRYNPATEASSCWVRVSQAWAGPGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRT
                     YHEDNRSPGDLPGTKTQMTIRSKTYKGSGFNELRFEDATDKEQVYIHAQKNMDTEVLN
                     DRTTDVKHDHTETIGNDQKITVVKGQTVQ"
BASE COUNT          432 a          406 c          640 g          362 t
ORIGIN      
        1 gaagcataaa tctgagtcaa caacatcctg cctccgtgca ggatgttgtt tttgtacgtg
       61 taaaaaatga gtgtcagtaa ggagttacgc tttcatgagg gagatggtgg atgccggaaa
      121 cccgggcggt agcgtacctg aaaatgtaaa gatgagtgta acgctgcact ggcacccttt
      181 gctgcatggt gggaatcatt tggtccgtga atgaatcagg gaggtcatta tgtccttaaa
      241 aggtcttcgc tttacgctgg aggttgacgg ccaggagccg gacacctttg cagtggtgaa
      301 tttcaggtta atccagaacc agtcttatcc gtttgtgatg agtgtggatg tcgccagcga
      361 ttcttttatg cagacggcgg agatgctgct ggagaagaag gcaacgctaa ccatctggca
      421 gggcgtcatc ccgcagcgtt atgtcacagg cgtggtggcg ggctttggca tgcaggagaa
      481 caatggctgg cagatgcgtt accacctgtg tattgagccg ccgttatggc ggtgcgggct
      541 gcggcagaac ttccgcatct tccagcagca ggatatccgg acgatatcgg ccacgttact
      601 gaacgagaac ggcgtcacgg agtggacgcc gctgttttat gaggaccatc cggcgcggaa
      661 gttctgtgtc cagtacgggg aaagcgacct ggcgtttctg gcgcggctgt gggcggagga
      721 ggggattttc ttctttgagc ggtttgcggc ggacagtccg gagcagaagc tgacgctgtg
      781 cgacgatgtg gcggggctgt cacaggcggg agagtttccg tttaacccgg acgcatcgac
      841 aggagcggag acggagtgtg tcagtatgtt ccgttatgag gcgcatgtcc gcccgtcgtc
      901 ggtgcagagc caggattaca cgtttaaggt gccggactgg ccggggatgt atgagcagca
      961 gggtgagagc ctgaacggac agcttgaaca gtatgaaata tttgattatc cgggaaggtt
     1021 caaggatgag cagcacggca aggatttcac gctgtaccag atggagagcc tgcgcagtga
     1081 tgcggaaaag gccacagggc agagtaattc gccgaagctg tggccgggaa cgcggtttac
     1141 gctgacgggg catccgcaga agatgctgaa ccgtgaatgg caggtggtac agagcattct
     1201 gtcaggagac cagccgcagg cgcttcacgg cagccagggg agaggaacca cgctgggcaa
     1261 tcagttagag gtgataccgg cggaccggac gtggcgtcca cggcagcaga gcaaaccgaa
     1321 ggtggacggg ccgcagagtg cgatagtgac ggggccagcg ggagaggaga ttttctgcga
     1381 cgaacacggg cgggtacgtg tgaagtttca ctgggacagg tataacccgg caacggaggc
     1441 gagttcgtgc tgggttaggg tatcgcaggc gtgggcgggg ccggggtttg gtaacctggc
     1501 gataccgcgc gtgggccagg aggtgattgt tgacttcctg aacggggacc cggaccagcc
     1561 gataatcatg ggcaggacgt accatgagga caaccgttca ccaggggacc tgccggggac
     1621 gaagacgcag atgacgatac ggtcgaagac ctataaagga agcggtttta atgagttgcg
     1681 ctttgaggat gcgacggata aggaacaggt ctatatccac gcgcagaaga acatggacac
     1741 ggaggtactt aacgaccgga cgacggatgt gaaacatgac catacggaga ccatcgggaa
     1801 cgaccagaag ataacggtgg tgaaggggca gacggttcag
//
LOCUS       DAZGJT010000258         1816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZGJT010000258 DAZGJT010000000
VERSION     DAZGJT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.264"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            130..330
                     /locus_tag="ACHKFK_004920"
     CDS             130..330
                     /locus_tag="ACHKFK_004920"
                     /inference="COORDINATES: protein motif:HMM:NF033650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO9055047.1"
                     /translation="MKTCLLKHYYKYAHLASKAERLNNYILAEKLWRRAYVFCCDADT
                     EWVSRRLAFCKKYLLRNGVDTK"
     gene            complement(470..840)
                     /locus_tag="ACHKFK_004921"
                     /pseudo
     CDS             complement(470..840)
                     /locus_tag="ACHKFK_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843781.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
     gene            complement(1069..1671)
                     /locus_tag="ACHKFK_004922"
     CDS             complement(1069..1671)
                     /locus_tag="ACHKFK_004922"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9055048.1"
                     /translation="MNSFQKTKQRGLSLIEAAMALALSAVVVSGVMYYMSTANENLQN
                     RKVTEMFISIAQHINALYSNQPKSAYTELTRDSGYQVLKKFFPGGEEKSIINRSGETS
                     KGITLNGIPGVFSLYGRGCSDSIRGNSTCAVVQYWIPNSYSENDAYNQCVAVISKNFG
                     DSILAKQANGSGRSVEGSNTDIQEISTICKNPSGITLLIR"
BASE COUNT          529 a          339 c          346 g          602 t
ORIGIN      
        1 aaaatatttt ctttattgtt actcgctcct aattcgtgga ataaatattt acccgtgtct
       61 ttttggttta tttttttatc tattcatatt gttcttttgt gttatgcttt taagtaagga
      121 gggttggtta tgaagacatg tttattgaag cattattata aatatgcgca tcttgcctca
      181 aaagcagaaa gattaaataa ttatatttta gcagagaaac tatggcgaag agcatatgtg
      241 ttttgttgtg atgcggatac tgagtgggta tcacgacgct tagcattttg taaaaaatat
      301 ttattgagaa atggagttga tacaaaataa ttgactttat ttcttggtac aatactaata
      361 catatgatgc aattacatgt ctaatttttt tgtaaaacat ttatatctaa gttcatgaac
      421 tgatcgttct aattttgtga tgtatgtcaa gtttttatat tcagtaatat taccttttac
      481 tcgatgatcg attgtttagc acctctccga caactggagc agaggtaaat gcatttggcc
      541 agcccgcata gaagacggcc tgggtaacga tctcaccagc ttcctcaaca gaaagtccat
      601 tatccattgc tctattgagg tgatagccaa tctgcgcgct cagtccggat gcgattaatg
      661 cgctgaccgt aatcaggctt ctgtcgcggg gtttcagcgc gggtctttgc cagagatcca
      721 ggaacagtgg atctgcagtg gatttaacca ggccaggaga tattgggcca acattttttc
      781 tactgtctct gaacactgat tttttgcttc ctgattgagc ggaagcaggt ccggataagc
      841 atctcttttc cctgccataa accaccgggt ccgacactcc ccctggtttc attcaactaa
      901 gccttcatca tcgtcgtaat attgaaccag tgaagtggca cactgaattt ggacacctga
      961 acagaggtga tatgctcacc tctgttcagg tgttccaatc actccgtatc actcagatgt
     1021 tatttaacat aatctatctt gtgcgcacta ttattgttat tcactgtgtt aacgaataag
     1081 taatgtaatg cctgacggat ttttacaaat agtactgatc tcttgtatat cagtgttact
     1141 accttctaca cttctacccg aaccatttgc ttgtttggca agaatggaat caccaaaatt
     1201 ttttgaaata acggcgacgc actgattgta agcatcgttt tcgctgtaag aatttgggat
     1261 ccaatactgc acaactgcac aagtactatt ccctctaatg gagtcggaac agcctctgcc
     1321 ataaagagaa aagacaccgg ggataccatt taatgtaata cctttcgatg tctcgcctga
     1381 tctatttatt atagattttt cttctccacc tgggaaaaac ttttttagca cctggtaacc
     1441 ggaatctcta gtaagctcgg tgtaggcaga tttaggttgg ttgctataaa gtgcattaat
     1501 atgttgggcg atggagataa acatttccgt tacttttctg ttctgtaaat tttcattggc
     1561 tgttgacatg taatacatga caccagaaac gactactgca gaaagagcaa gcgccattgc
     1621 agcctcaatt aagctaagac cgcgttgttt tgttttctgg aaactgttca tttttgacca
     1681 caaagatatt tattgatgca taaaaaattc tttataagta aaacatatcg gttgtaaaaa
     1741 aaaaacaagg aagaacctat atcttattca aaaaaaagga taatcaccta agagttgtaa
     1801 taatttgtaa aaaaat
//
LOCUS       DAZGJT010000259         1811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZGJT010000259 DAZGJT010000000
VERSION     DAZGJT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.265"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..549
                     /gene="cmtB"
                     /locus_tag="ACHKFK_004923"
     CDS             106..549
                     /gene="cmtB"
                     /locus_tag="ACHKFK_004923"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO9055049.1"
                     /translation="MRLIDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKGSTISNGPYYIIAPGVAMPHARPECGALKTGMSLTLLKQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            577..>1811
                     /gene="cmtA"
                     /locus_tag="ACHKFK_004924"
     CDS             577..>1811
                     /gene="cmtA"
                     /locus_tag="ACHKFK_004924"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO9055050.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGSVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYIDKSLEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPAPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETKSEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENV"
BASE COUNT          431 a          388 c          464 g          528 t
ORIGIN      
        1 ctcgtggagt ttctcctttc atgtgtccaa ttgctttcgt taatttgtac gcgtatttta
       61 tttttaccgc cggactgtac cctaaaaata aaataaggat aatttatgcg gcttattgat
      121 tattttccag agtcatcaat ctcagttata cattcagcaa aagattggca ggaagctatc
      181 gatttctcga tggtatcatt gctggataaa aactatatca gcgagaatta cattcaggca
      241 attaaaggtt ccaccatcag caatggtcct tattatatta tcgcaccagg cgtggcaatg
      301 cctcatgcgc gaccggaatg tggagcgctt aaaaccggga tgtctttgac gttacttaaa
      361 caaggcgttt attttccggg gaatgacgaa ccgattaaat tactcatcgg actttctgct
      421 gccgatgccg attcgcacat tggtgctatt caggcgttaa gtgaattact gtgcgaagaa
      481 gaaatactcg aacaactctt aaccgcatca tcagaaaaac aattagcgga cattatcagc
      541 cgcggataat gactctctcc tctacaggat aatattatgg aaaacaagtc tgctcgtgca
      601 aaggtccagg cttttggggg atttttgact gcaatggtca tccccaatat cggtgctttt
      661 attgcctggg gatttataac ggcattattt attcccaccg gttggctgcc gaatgaacat
      721 ttcgccaaaa ttgttggtcc gatgattacc tatttattgc ccgtgatgat tggttctacc
      781 ggtggtcatc tggtcggcgg taaacgcggc tcggtcatgg gcggaattgg tacgattggt
      841 gtgatcgtcg gcgctgaaat cccgatgttc cttggctcga tgattatggg gccgctcggt
      901 ggtctggtga ttaaatatat cgacaagtcg ctggaaaaac gcatacctgc tggctttgag
      961 atggttatca ataacttctc attaggtatc gcggggatgc tcctttgtct gctgggtttt
     1021 gaagttatcg gcccggcggt gttaattgct aatactttcg tcaaagagtg tattgaggcg
     1081 ctggtacatg cgggttatct acctttgctg tcagtcatca atgaaccggc gaaagtgctt
     1141 ttccttaata atgcgatcga tcagggcgtc tattatccgc tgggaatgca acaggcttcg
     1201 gttaacggta aatccatctt cttcatggtg gcctccaatc cagctcctgg tctggggctg
     1261 ctgctggcgt ttaccttgtt tggtaaaggg atgagtaaac gttctgcgcc cggggcgatg
     1321 attattcact tcctcggtgg gatccacgaa ctgtatttcc cgtatgtgct gatgaagccg
     1381 ctgaccatta ttgccatgat tgcgggcggt atgtctggca cctggatgtt taacttactg
     1441 gacggtggtc tggtggctgg cccaagtccg gggtctatct ttgcttacct ggcactgacg
     1501 ccgaaaggct cgttcctggc gacaattgcc ggtgttacgg taggtaccct ggtgtccttt
     1561 gctattactt cgctgatact gaagatggaa aaaacggtgg aaacgaagag cgaagatgag
     1621 tttgctcagt cagccaatgc ggttaaggcg atgaaacaag aaggtgcatt ctcgttaagc
     1681 cgtgttaagc gtattgcctt tgtttgcgat gcagggatgg gctccagtgc gatgggcgca
     1741 accaccttcc gtaaacgcct ggaaaaagcg gggctggcaa tagaagtaaa acattacgcc
     1801 atagaaaacg t
//
LOCUS       DAZGJT010000260         1805 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZGJT010000260 DAZGJT010000000
VERSION     DAZGJT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.266"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1805)
                     /locus_tag="ACHKFK_004925"
     CDS             complement(<1..>1805)
                     /locus_tag="ACHKFK_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HFO9055051.1"
                     /translation="VGLFFSSLSYAKNTEIPSYEEGISLFDVEATLQPNGVLDIKENI
                     HFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEPWHLDWHIGLMSI
                     VVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNNWPFEIYKTRFSLELP
                     DIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVLYRWPHALLGNAP
                     APQTTNIFSHLLLPSTSSLLIWFPCLFLACGWLYLWKRRPQFTPVNVIETDVIPPDYT
                     PGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLKDQYDKNQQILIRVNEGATRNNAVLL
                     PAEQLLLEALFRKGDKVVLTGRRNRVLRKAFLRMQKFYLPRKKYSFYRPDAFLQWGGL
                     AILAVILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFARDDDKPCVNRVII
                     TLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVFLTGYLTGMGYIFLPKFTQTG
                     QQRYAHGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAVSANLGREWAARITAS
                     LTAAVRAPEIARSGVLFSLQTHLSCGAYTSLLGRS"
BASE COUNT          535 a          454 c          392 g          424 t
ORIGIN      
        1 agctccgccc caacaaactg gtataggccc cgcaactcag gtgcgtctgt aatgagaata
       61 aaacgccact acgggcaatt tccggggcgc gaacagccgc tgtaagtgat gcggtgatac
      121 gtgctgccca ttctctgcca aggtttgccg agaccgccca acctaacaat gcgtaatcca
      181 gtttccgtgt ttcccctttc cgccgccgcc cactgtgtgt tgccgcctct ttacgcgcaa
      241 gatagttaac gatagcttca ccgtgggcat aacgttgctg cccagtttgg gtaaactttg
      301 gcagaaaaat ataccccatg ccagtgagat aaccggtcag gaaaacagcg ctaaaataac
      361 ccgctggcat ataccagtaa aagaatacat ctccgacatt gatatagaga gagtaaaagg
      421 ccacgcctga acaaatcagc ggtaaaaaca aagtgatgat tacacgatta acgcaaggct
      481 tgtcatcatc gcgggcaaac aaaagctcaa gggaacaaaa taaaaaagga ataatccagc
      541 agatcataat aaacatatcg cctaccagac tcattcctgc ccaacctacg ggactcaggt
      601 taccgtagag aatgaccgcc aatattgcca gtccacccca ttgcaaaaac gcatcaggtc
      661 ggtaaaacga atacttttta cgcggcagat aaaatttctg catccgtaaa aatgccttgc
      721 gtaagactct gttgcgtctc cccgtaagaa cgaccttatc gcctttacga aataacgctt
      781 ccagtaataa ctgctctgcg ggcagtaata ccgcattatt tctggtggcg ccttcattaa
      841 cacgaatcag gatttgctgg ttcttgtcat actgatcttt cagatgaatt tttcccttca
      901 cgatcagatt tacgatatcg gcacaaaaac ctttatcgtc gtaaaccagc ttcgcatcga
      961 gacgtaacat gccgggcgtg taatctggcg gaatgacatc ggtttcaatc acatttaccg
     1021 gcgtaaattg cggcctgcgc ttccagagat ataaccatcc acacgccagg aacagacacg
     1081 gaaaccaaat taacaacgat gacgtagagg gtaaaagaag atggctgaaa atattcgtcg
     1141 tttgtggtgc cggggcatta ccaagtaaag cgtgaggcca gcggtagagg accgtgaagt
     1201 cttcacgata aaacggatcg cgggattcaa ttcttccgtc ctcaaggatg cggccatttc
     1261 gatatgtgtc gccctcttct ccagtaaaga gatcaatttc gctaaatgga ttacccgcaa
     1321 tatcaggcaa ctcgagtgaa aatcgggtct tatagatttc aaacggccag ttattaccag
     1381 tcacgttcca gattagcaga tcagaatccc cctcacgcag gaaagcattt ttaacctgat
     1441 aatgaatttg ataatgatag tcgccttgag gcaagaaacg ttgtttatcg cccacgacaa
     1501 tactcattaa cccgatatgc cagtcaagat gccagggttc aggaatacca tcacgggtga
     1561 cgccaacaat atgatagttc agcagtgcag cgtccccatc aggctgcatc cagagtcgcg
     1621 gtaaatcacg ataaaatccg tgcttaatct gctgatttcg cgcctgaaaa tgaatgttct
     1681 ctttgatgtc gagcacccca ttcggttgca gagtggcttc aacatcaaag agcgagatcc
     1741 cttcttcata agaagggatc tccgtgtttt tcgcataact caacgaagag aaaaaaagcc
     1801 caact
//
LOCUS       DAZGJT010000261         1762 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZGJT010000261 DAZGJT010000000
VERSION     DAZGJT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.267"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            93..947
                     /locus_tag="ACHKFK_004926"
     CDS             93..947
                     /locus_tag="ACHKFK_004926"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009025 - tagatose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HFO9055052.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLRVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            983..>1762
                     /locus_tag="ACHKFK_004927"
     CDS             983..>1762
                     /locus_tag="ACHKFK_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO9055053.1"
                     /translation="MKNIKLLGSAMLVSLALFSQSSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSVSIANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIK
                     ADKAGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLHGKGTVAIISYPALQ
                     SVVARVDGFKKGLSDTPDIKIVAEQPGITRAEALTTAQNIMQANANLNGLFGFGDDAA
                     LAAVIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQ"
BASE COUNT          479 a          360 c          443 g          480 t
ORIGIN      
        1 gctgcttttg ctgtgctagt ttgctttcaa aatgaaagta aagattctct ttcttcattt
       61 tgtaaggcga acgacaaata acaggagctg cgatgtatat tatttcaagc aaaaacatgc
      121 tgaaaaaagc ccagtcagag ggatatgccg tccctgcttt caacattcat aaccttgaaa
      181 ctttacaggt agttgttgag agcgctgcac aacttcgctc tcccgttatg ctggcgggaa
      241 cacccggtac atatcgttat ggtggcgtgg gaagtttaat ttcgttagta caatcgctgg
      301 ctcgcgagta caacctgccg ttagtcctgc atctcgacca tcatgaagag agtgatgaca
      361 tttttaacaa ggttcgcgca gggatccgtt ctgtcatgat tgatggctcc catttgccat
      421 ttgaagacaa cattgcgcga gtgcaagaag ttaccacctt ttgccatcgc tttgatgtta
      481 gcgttgaggc cgagctaggg cgtcttggcg gccaggagga cgatctgcgg gtggatgaaa
      541 aagattccgc ttatacctca ccagccgcag ctgcagagtt tgttcaacgt acccaaattg
      601 actccctggc ggtagcaatc ggcacagcac atggtttata tgcgcatgaa ccacgactgg
      661 attttgtgcg gctggaacag attcgtcagc gagtggagat tccgctggtg ttgcatggtg
      721 cttcgggctt atcggaacag gacgttcagc actgtattcg tcgcggtatt tgtaaagtga
      781 atgtcgcgac agaattgaaa attgcgtttg ccgatgcgtt aaaaggatat ttctacaaca
      841 atcctgcggc aaatgatcca cgtcactata tgcagcccgc aaaggccgcc atgaaagaag
      901 tcgtaactcg tattattggc gtctgtggta gtgaaggaaa aatttaagac gttgtaccct
      961 acaaaaataa tgaggattgt ttatgaaaaa tattaaatta cttggttcag ctatgttggt
     1021 gagcctggca ttgttctcgc aaagttcgtt ggcaaaagag tataaaattg gtgcgtcatt
     1081 attaacacaa caacatcctt tctatattga tcttgcgaat gccatgaaag aagaggcaaa
     1141 aaaagacaat gtcaatttaa gtgtctctat tgccaatcag gatttaaaca aacaactttc
     1201 tgatgttgag gattttatta ctaaaaaagt ggatgccatt attatctctc cggtcgattc
     1261 gaaaggcgtc caggcagcga taattaaagc cgataaggcc ggtattcccg ttatcaccgt
     1321 tgacgtcgct gcggaaggcg ttccggtcgt atcgcatgtt gcgaccgata actatgcggg
     1381 aggcgttgaa gcaggcaagt taatgggtaa acttttgcat ggtaaaggta ctgtagccat
     1441 tatttcttat ccggcactac aatcggtggt tgcgcgcgtt gatggtttta agaaagggtt
     1501 aagcgataca ccggatatta aaattgttgc tgaacagcct ggtattacgc gggctgaagc
     1561 gttaacgact gcgcaaaaca ttatgcaggc aaacgccaac ctgaatggtc tgtttggctt
     1621 tggtgatgat gcggcgctgg cagcggttat cgcagcaaaa tctgcgcata acgacaacat
     1681 caaaatcatt ggttttgacg gcatgaaaga agcgcgtgat gccgttgaca gtgaaaaaac
     1741 gtttgcggcc gtgattcggc aa
//
LOCUS       DAZGJT010000262         1750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZGJT010000262 DAZGJT010000000
VERSION     DAZGJT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.268"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..179
                     /locus_tag="ACHKFK_004928"
     CDS             <1..179
                     /locus_tag="ACHKFK_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO9055054.1"
                     /translation="KLTRIVVHVDLQPIADELHGDYINDKSFKRHFQQWLNSLWQEKD
                     RLLTSLMSSQRQEK"
     gene            complement(220..1650)
                     /gene="yihF"
                     /locus_tag="ACHKFK_004929"
     CDS             complement(220..1650)
                     /gene="yihF"
                     /locus_tag="ACHKFK_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO9055055.1"
                     /translation="MIRKSATGAIVALAVIWGGGTWYTGTQIQPGVEKFIKGFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKIVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGPFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDDSSLSNLDIEGKVEDIVLQFSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEVVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDMK
                     LPLNVATETAKQLNLSEGMDAEKAQKRADKQISGMMTLGLMLQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT          485 a          384 c          343 g          538 t
ORIGIN      
        1 gtaaactgac gcgaattgtc gtacatgtgg atttacagcc tattgccgat gagttacatg
       61 gcgactacat caatgacaaa agctttaagc gccattttca gcaatggctg aactcattgt
      121 ggcaggaaaa agaccggctt ctgacatcgt tgatgtcatc acagcggcag gaaaagtaag
      181 ttttactcgc ctctgaatga gcagaggcga gtgagtattt taatgaacaa aacgtccggc
      241 acgagacata aattcttctt cgctcatctc ctgtccgtta aaaacaactt tacccggtgt
      301 ataacgcagt tgcagcgagg cggtattgtt gtcaatcgtg attaactgta acatcagacc
      361 taatgtcatc atcccactga tttgcttatc agcccgcttt tgcgcttttt ccgcatccat
      421 cccttcagat aaattaagct gttttgcggt ttctgtggcg acattaagcg gtaacttcat
      481 atcaaaattg agcgatttaa tatctttgtt tgtggatgat gaagatttgg ctgggtcggc
      541 aatactgata tccagattgg catttagttc gccgagtgcg ttcttccatc ttactggttg
      601 tttaatggtc ggctcacttt tttgtaataa cggtaaatac tctttgaaca atgcagcatt
      661 cacttcttgc agagcaactt catcgtttgc taactcaggg tgtgcagcaa gctgcttctg
      721 catcgcaatg ttatactgga tgataaattg gcgcaccgct gagggatcaa tagattcagc
      781 aatcaatgac cactcaccac taccgagttg ctgattccct tttgttaact tatcaagttc
      841 gtaggtcaga ttgacattga tataatcttt gtctttaacg cgatccagcc tggtttttgc
      901 aacgaaagca tcgatttggg caacgacttc cccctggttg ataatattaa cctgattaaa
      961 tttcgactcg ctttcaccaa ccggaaattt cttttcttct aatcgtgtca gagaatcaat
     1021 ggtaaaactt ttcgccgtta ctttgttcat tggtgaaaat tgcagaacaa tatcttcgac
     1081 tttgccttca atatccaggt tagacaatga actatcatca ccattaaaag taaactggcc
     1141 ttcaccaaaa gaaaactttc catactctgc aggggcaaca tctaatgtcg tggagaatgg
     1201 gccaccaaac gcgaatcgca atgtcgcttc cacgggcgat ttatttttcg cggcgataaa
     1261 tagcggttgt gtcagctcat tattcactaa gttcactttt gccgctgcca gtgctgggat
     1321 gacattacca tgcattaaca ttgtgatggg caacggaccg tgctcaacat ccacatcaaa
     1381 tacaattttc tggcctggct tgatattgag atcgggtgca ccgttatcaa atgtcatttg
     1441 catttgaaaa cgggaattaa aaaagccttt gtcaaaattt ttataactta acgtcatatc
     1501 gtaggcatgt tcacctttct ttttagcatc gttaaaacct ttaataaact tttcgacacc
     1561 aggctgaatt tgcgtacctg tgtaccatgt gccaccaccc cagattacgg ctaacgcaac
     1621 aatggcacct gtagctgact tacgtatcat ataaatattc cctgataaaa cagcaaaact
     1681 ttgaatatcc acttatgccg aaataatgtc cagcagcagg aagcattatc agaatgtatt
     1741 tatatttatt
//
LOCUS       DAZGJT010000263         1709 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZGJT010000263 DAZGJT010000000
VERSION     DAZGJT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.269"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..740
                     /locus_tag="ACHKFK_004930"
     CDS             <1..740
                     /locus_tag="ACHKFK_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO9055056.1"
                     /translation="VIVLFSLLFSTAQAGLVIGGTRFAYPENQSSISVELKNTSDRDM
                     LVKVAVSPDDARQLTGTVESLPSYSETVFIATPPLFVLKPDKNTKIRINRVGGSLPTD
                     RESLFILNVAALPTLENSHSVKIDNQLQIAVRNRMKIFYRPSNLSEDPNMSYQKLRWA
                     RKNEMVTVYNPGPRYVTLYNLHVDGKVIDGGMVAPFSHRQQSWCKSQGVCEIAWQTLD
                     VYNNVLPILKVKLNPSQLDFTGFAFSQ"
     gene            759..>1709
                     /locus_tag="ACHKFK_004931"
     CDS             759..>1709
                     /locus_tag="ACHKFK_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9055057.1"
                     /translation="MRIHTYWYRRYFILLIIIFSNVLSSIANAEDMGRERAYCYPGSP
                     SNNTTPASFSYNFGTIVVSDVNKNAPGTVLPSQIWKVGTYKAYCNSLDDYEIYFSAVS
                     GIDPSGASGDHQGSDVFIPLTHEISVSTHIKLYNQNGTMTDKIVPFENYNTNYPGDRS
                     KPSNWASGTEGYIKIRIDKKIISDVSLSNVLLVSLYVSQIPTEHGPIPVFNAYIGNLN
                     IQVPQGCTINEGTSFTVNMPDVWASELSRAGAGAKPAGVTPVATTIPINCTNKDTDAV
                     MTLVFDGNISATRDTNGKQSIIQAQDNPDVGIMIMDSQQNS"
BASE COUNT          522 a          309 c          343 g          535 t
ORIGIN      
        1 tagtcatagt attattttcc cttttattct ctacagcaca agcaggctta gtgatcggtg
       61 gaacgcgttt tgcgtatccg gaaaatcagt cctcaatatc tgttgaactc aaaaatactt
      121 cagacagaga tatgttagtc aaggttgcag tctcccctga tgatgctcgt cagttgacgg
      181 gaacggttga atctttgccc tcatattctg aaacggtatt tatcgcaact ccgccactgt
      241 ttgttttgaa gcctgacaaa aatacaaaaa taagaattaa tcgtgttggt ggcagtttac
      301 ctaccgaccg ggaaagttta tttatactga atgttgcagc attaccaacg ttggaaaata
      361 gtcattcagt caaaattgat aatcaattgc aaatagcagt ccgcaatcgg atgaaaatat
      421 tctaccggcc atcaaatttg tctgaagacc cgaatatgtc atatcaaaaa ttacgctggg
      481 cacgaaaaaa tgaaatggtt accgtttata atccaggccc cagatatgtg acactctata
      541 acctacatgt tgatggtaaa gttatagatg gtggtatggt cgcaccattt agccatcgcc
      601 aacaatcatg gtgtaaatcg caaggggttt gtgaaatagc atggcaaact ttggatgttt
      661 ataataatgt attgccaatt ttgaaagtaa aattaaatcc ttcacagttg gattttactg
      721 gctttgcatt tagtcaataa tatatctgag gacatgatat gcgtatacat acttattggt
      781 atagaagata tttcatttta ttgattatta tattttcaaa tgttctttct tctattgcta
      841 atgctgaaga tatggggcga gaacgtgcat attgttatcc gggttcaccg agtaataata
      901 ctacgcctgc atccttttct tataattttg gtactatagt ggtttctgat gtcaacaaaa
      961 atgcgcctgg cactgtattg ccatcacaaa tctggaaggt tggaacctat aaggcttatt
     1021 gtaattctct tgatgattat gaaatttact tcagtgctgt ctctggaata gatccgtctg
     1081 gtgccagtgg tgatcatcaa gggagtgatg tatttattcc actcacccat gaaatatctg
     1141 tctctactca tataaaactt tataatcaaa atggcacaat gacagataaa attgtgccat
     1201 tcgaaaatta taataccaat tatccggggg acagaagcaa accatctaat tgggcatcag
     1261 gtactgaagg atatattaaa atcaggattg ataaaaaaat tatatctgat gtttcattaa
     1321 gtaacgtatt attggtgtca ttatatgtca gccagatccc taccgaacat ggtcctatcc
     1381 ctgtctttaa tgcctacata ggaaacttaa atattcaggt tccgcaaggt tgcactatta
     1441 atgagggtac gagttttact gttaatatgc cggatgtgtg ggccagtgaa ttgagccggg
     1501 ctggtgccgg agcgaagccc gctggtgtta ctcctgtagc aacaactatt ccgattaatt
     1561 gtacgaataa agatacagat gcggtaatga cgttggtatt cgacggtaac atttccgcca
     1621 cacgtgatac caatgggaaa caaagtatta ttcaggcaca agataatcct gatgttggta
     1681 ttatgattat ggatagtcag caaaactcc
//
LOCUS       DAZGJT010000264         1685 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZGJT010000264 DAZGJT010000000
VERSION     DAZGJT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.270"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..1614
                     /gene="ltrA"
                     /locus_tag="ACHKFK_004932"
     CDS             106..1614
                     /gene="ltrA"
                     /locus_tag="ACHKFK_004932"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFO9055058.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSPKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          436 a          418 c          484 g          347 t
ORIGIN      
        1 ccgcgatggg gaaagcccat tacatggcga agggaagcag tttgaatgtg tttgcgacgt
       61 gaattaactg acctaacgag gtgaagacct ttgataatca gcgaaatgca acgcaagctt
      121 gccacatggg cagccaccga tccgtcccta cggattcaac ggctgctgcg tctaataaca
      181 caaccagaat ggctggctga agcggcgcgg atcacgcttt caccaaaggg ggcccatacc
      241 cccggcgttg atggcgtgaa caaaacaatg ctacaggcca gactggctgt tgagctgcaa
      301 atcctcaggg atgaattact ctcaggccac taccagccct tgcccgccag acgggtttac
      361 atccctaaaa gcaacggcaa actgcgacca ctgggtatcc ccgcgttgcg ggatcgtatt
      421 gttcagcggg ccatgctgat ggcgatggag ccgatatggg agagtgattt tcatacgctc
      481 tcatatggct tccggcctga gcgcagtgtc caccacgcga tccgcacggt gaaattacag
      541 ctcacagact gcggtgaaac ccggggacgc tgggtgattg aaggcgacct gtccagttac
      601 ttcgacaccg tacatcatcg actgctgatg aaggctgtac gccgcaggat cagtgacgca
      661 cgtttcatga ctctgctgtg gaaaaccatc aaggcgggac atatcgatgt cggtctcttt
      721 cgggcggcca gtgaaggtgt gccacagggc ggtgttatat cgccgctatt atcgaacatc
      781 atgctgaatg agttcgatca atacctgcat gagcgctacc tgagcgggaa agccagaaaa
      841 gatcggtggt actggaataa cagtatccaa cggggccgaa gtacggcggt cagagaaaac
      901 tggcagtgga aacccgcggt ggcgtactgc cgctatgccg atgattttgt cctcatcgtc
      961 aaaggcacca aagcacaggc ggaagccatc agggaggagt gtcggggtgt gctcgaaggc
     1021 agtctgaaac tcaggctgaa catggataag actaaaatca cccatgttaa tgacggcttt
     1081 atctttctgg ggcacaggat cattcgcaaa cgcagtcgtt atggcgagat gcgagtggtc
     1141 tcaacgatcc cgcaggagaa agccagaaac ttcgccgcat cgctgacagc actgttatca
     1201 ggcaactaca gtgaaagcaa agtcgatatg gctgaacaac tcaaccgaaa actgaaaggc
     1261 tgggccatgt tctatcagtt cgttgatttt aaggccaaag tcttcagtta tatcgaccgt
     1321 gtcgtgttct ggaagctggc tcactggctg gcccgcaaat accgtacagg tatcgcttcc
     1381 ctgatgaggt ggtggtgtaa atcaccgaaa ccgggtcaga gcaaaacgtg ggttttattt
     1441 ggtaaaacca atcacggcaa gctcagcggc gaaatactgt accggttggt ggggcaaggc
     1501 aagaagctgt tccgctggcg gctacccgaa ggtaatccct atctgaggac ggagaccaga
     1561 aacacgtata catcgcgctt tacagaagtg gcaatggcgt tcgccagcat ttaaatggag
     1621 agccggatgc gctgaaaggt gcacgtccgg ttcggggagg agaggcaggg aaatagtccg
     1681 actac
//
LOCUS       DAZGJT010000265         1666 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZGJT010000265 DAZGJT010000000
VERSION     DAZGJT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.271"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1383)
                     /gene="ycjM"
                     /locus_tag="ACHKFK_004933"
                     /pseudo
     CDS             complement(1..1383)
                     /gene="ycjM"
                     /locus_tag="ACHKFK_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
     gene            complement(1593..>1666)
                     /locus_tag="ACHKFK_004934"
     CDS             complement(1593..>1666)
                     /locus_tag="ACHKFK_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309912.1"
                     /note="rhodanese; catalyzes the formation of thiocyanate
                     from thiosulfate and hydrogen cyanide; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO9055059.1"
                     /translation="EMGYTHVENAGGLKDIAMPKVKG"
BASE COUNT          441 a          386 c          372 g          467 t
ORIGIN      
        1 ttgttcagtt ctgtcgttat ctctttacta gagtattttt tacggttaat cgcacgatta
       61 tatccaagtt tttcgacacc tgcgtaatca ttacgcgacc ccagaatgct ttgaatatat
      121 atcgctggca caccggggaa acttaacaaa atcgcatggg cgagaataaa cctggcacac
      181 cgttcttcgt cgctacactc acggcggctc aacgcatcca tataggtgac attcatttca
      241 taggggctac gcgtaccgtc gggattattt ttccagttta ctaatgctcc ttcctgctgt
      301 aacacctcga ccagcgctaa tatttcgttt tcgggcaaca acccccgcag tgggttaagc
      361 ccgatgccat cgtgagaggc aaggaagtta aaccaggtgg tgttgctgga aggtagtgtc
      421 aggttttgcg cccacgcaca aagtgcctca acgttctgtt tttgcaccgc atgcagcacc
      481 agtggcggca gcgagaactg gtacaccata tgtgccttcg tcatcaccgt tgccaaagta
      541 agcaatgttg tctttgtgcg gaacattggt ctcggtaatg atcactgtgc ctggcgcgac
      601 gttatcaata atcgaccgta acagtttgat aatcagatgt gttttttcca gatggatgca
      661 gtttgttccc ggctctttcc acataaagcc aacggcatcc agacggacat actctgcgcc
      721 cttttccagg tagcacagca atacatccac catcgccagc aacacttcag ggctacggta
      781 attgaggtca atttgatcgt cgctaaaggt ggtccataaa tggcgcattg aatgatcgcg
      841 catctggaat ggcgttaata acggtaacgc acgcgggcga gtgacggcgc tgagatcggt
      901 tttcggatca acggcaataa aaaaatcttc aaaacctggc tgctgttgta aatagttttt
      961 aaaccattca ctttttgccg acatatggtt gcagacaaaa tcaaacatta aatggctgca
     1021 ttcaccgagt tgctgaatat cctgccactc ccccgcttca ctggcgacct gatgataatc
     1081 aattaccgaa aatccatcat cagatgacca tggataaaaa ggtaataaat gaacatgtga
     1141 aaaaattctt tgcagccatt gagggtaaaa ctgattaaat gtgggtagtg gctttaaatc
     1201 attactgtga aattgatcgg cataggtaat taacacgaca tcactttcat cccagtgttt
     1261 tttacgtcgc tgtgtaatta atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa
     1321 atgagtcgcg gtaaatgttc caccgtagat ttcgtccagg taatccgtaa ttttctgttt
     1381 catgttgcct ttccgtggta gcggtcccat gaaacgagag actactccgc ataaaataaa
     1441 ctgtcaattg agctcaaggg gtttagtcac gcaggaaatc tttttttacc tgggaaaaag
     1501 cgtatttgtg agctaacgca aataatctat ggggatgtaa atagggtgtg acagaaaaaa
     1561 cggcgcataa gcgccgctca tggtgaattc ttttaacctt tgaccttcgg cattgcgatg
     1621 tctttcaggc caccggcgtt ctcaacgtgg gtatatccca tctcgc
//
LOCUS       DAZGJT010000266         1625 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZGJT010000266 DAZGJT010000000
VERSION     DAZGJT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.272"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..544)
                     /locus_tag="ACHKFK_004935"
     CDS             complement(<1..544)
                     /locus_tag="ACHKFK_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFO9055060.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPS"
     gene            complement(564..911)
                     /gene="tnpB"
                     /locus_tag="ACHKFK_004936"
     CDS             complement(564..911)
                     /gene="tnpB"
                     /locus_tag="ACHKFK_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9055061.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            complement(911..1561)
                     /gene="tnpA"
                     /locus_tag="ACHKFK_004937"
     CDS             complement(911..1561)
                     /gene="tnpA"
                     /locus_tag="ACHKFK_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO9055062.1"
                     /translation="MNARKAALADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQIFADWPLSGEYSCMQLARALFPGRYPSAPTVTQPVKTEKIRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPTGEHETSLLPVTLTPE
                     PDNKIPAPAQEPEQINTPPDSLCCELVLPAGTLRLKGELTPALLQTIIREMKGSSH"
BASE COUNT          354 a          425 c          453 g          393 t
ORIGIN      
        1 ttgaaggtac tggtgcctgc acgatatggt cgcaccggca acaggcctgt ttcggacgtt
       61 gtgtttcgat aaccttaaag gcgctgctga taagctccag ttgctctgac acatcacatc
      121 ccagagaact gagttcacca ccacaggcag gacagcattc ctcttccggc cggataaccc
      181 gggtttcacg gggaagtgag gccggtaacg gtttacgggc tgaagactgg cgcagggcgg
      241 atggcagtac cgggtcatat tgctcaccca gcgtttccgc catttcttcc tgaagtgcgc
      301 tgattcgctc ctgtgcttcc tgtatctgcc gttcggtttt tgcacgaagt ttttctgagc
      361 ttttaccgaa ctgcatacgt tgcagtttcg caaccagcgc cttcagccgg ttgatttcgg
      421 aagcataagc cgccacccgc tgtgagagca ggcggttgta ttcagccatc tggcggatgg
      481 tgtcctgttg cgtctgcaac agtgcccgca ggcgggcgtt ctcatgagca agtgaggtgt
      541 ccatatcctc actttacaac gggttatatg cggattccag cgcgttccgt tcgtttcggg
      601 tgcttccagt tgataccttc aagaagcata gataactgag ccggagtaag gtgcaccttg
      661 ccgtcacggg taaccggcca gacgaagcgg ccccgctcca ggcgtttggt gaagaggcac
      721 agtccgtcac tgtcagccca caacactttt atctggtcac cccggcgtcc gcggaagatg
      781 aacagatgcc cggagaacgg gtcatccttc aggacgttct gaacttttga tgccaggccg
      841 ttaaaaccat ttcgcatgtc ggtgataccg gcaaccagcc agatacgcga accggcaggg
      901 agagatatca tcagtggctg ctccctttca tttcgcggat aattgtctgt aataacgccg
      961 gtgtcagttc acctttcagc ctgagagttc cggctggcag aaccagctca cagcacagac
     1021 tgtcgggcgg tgtatttatc tgctcaggtt cctgtgcggg tgccgggatt ttattatccg
     1081 gctccggcgt taacgtcacg ggaagtagcg atgtttcatg ttcgccggtc ggctgcaggc
     1141 cgccgttacg ccagagatgc cgccagttaa agagcaggtt atcgttgatt ccgttctcac
     1201 gagctatttg tgcgacacag gctccgggta aaagagtttg ctgaacaagc ctccatttaa
     1261 actccggtga aaatcggttc cggcggattt tctctgtttt cacaggctga gtcactgtcg
     1321 gggctgaagg gtatcgccca gggaaaagag cccgggccag ttgcatgcag gaatattcac
     1381 cggaaagagg ccagtcagcg aatatctggt gaaatcgcct caccagagaa aaaatggcgg
     1441 ttttgctgac accggtctgc gcagaaatgc gcgggtacga cagtgactcg tcaaatctca
     1501 gttgtagcac acgcaggatc aattccggat tatctgccag tgccgccttt cgtgcattca
     1561 tcttaaatgt ccttttactg cgaaaatgga cattagtatc ggaaacagga aagggaggcg
     1621 aaaga
//
LOCUS       DAZGJT010000267         1614 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZGJT010000267 DAZGJT010000000
VERSION     DAZGJT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.274"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..640
                     /locus_tag="ACHKFK_004938"
     CDS             128..640
                     /locus_tag="ACHKFK_004938"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO9055063.1"
                     /translation="MKRKNQHGFSLIEVSLSLILICFGMLFSFYFIDITKMISNSQKI
                     SEDFSLIKNYCIKESESMSPGSTSVIKHKRKGIYLPHGYLIFMQKRDDNSYMCLSLLE
                     NNKKSFNLDQLYIISFLTGVEGAYYEKGEIKKPSILFSPVKVSSLNIPEYQFSSSRMP
                     ALYFIGDERS"
     gene            842..1614
                     /locus_tag="ACHKFK_004939"
                     /pseudo
     CDS             842..1614
                     /locus_tag="ACHKFK_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1123..1239
                     /locus_tag="ACHKFK_004939"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          469 a          323 c          373 g          449 t
ORIGIN      
        1 ccaacctttt taaaaggcct atattataac attgatattg atggcaatta caccctaggt
       61 gatggaaata tatatgcaat tattcctccc cgttattcta aataccaacg atgccagtga
      121 ggttataatg aaaaggaaaa atcaacatgg attttcacta attgaggttt cattgtcttt
      181 gattcttatt tgctttggta tgctattctc tttttatttt atagatataa caaaaatgat
      241 cagtaactca caaaagatct ccgaggattt ttctctcata aaaaattatt gtattaagga
      301 aagcgaatcg atgtctccag gtagtacttc cgtaataaaa cataaaagaa aaggcattta
      361 cttgcctcat ggttacttga tatttatgca aaaaagggat gataattcat atatgtgttt
      421 atctctgctt gaaaataaca aaaaatcgtt caaccttgat cagttatata taatttcttt
      481 tttaacaggt gttgagggag catattatga aaaaggtgaa ataaaaaagc catcaatttt
      541 atttagtcct gtgaaggtca gttcattaaa tattccagaa tatcagttct catcatcaag
      601 aatgccagcg ctttacttta taggtgacga gcgctcctga ctaatatatc acggagtgat
      661 tctttagaat tctgaagagc tagtatgtag aagtactgtt tattatatcc ttaacgatat
      721 tacccatgca ataacagact ttatcattag atgttatagt atactcagga ttcattaata
      781 taactattga accgccccgg gaatcctgga gactaaactc cctgagaaag aggtaaacag
      841 gatgactaaa aatactcgtt tttcccccga agtccgtcag agggcgattc gtatggttct
      901 ggaaagtcag ggcgaatatg actcacagtg ggcggcaatt tgttccattg ccccaaagat
      961 tggctgtaca ccggagactc tgcgtgtctg ggtacgccag catgagcggg ataccggagg
     1021 cggtgatggc gggctcacca ccactgaacg tcagcgtctg aaagaactgg aacgtgaaaa
     1081 tcttgaactg cgccgcagta acgatatcct tcgccaggct tccgcttatt ttgcgaaggc
     1141 ggagttcgac cgcctctgga aaaaatgata ccactgctgg ataagctgcg taagctgtac
     1201 ggggtcggac cggtatgcag tgaactacat attgccccgt caacgtatta ccactgtcag
     1261 caacagcgac atcatccgga taaacgcagt gcccgtgcgc agcgcgatga ctggctgaag
     1321 aaagagatac agcgcgtata cgatgaaaat catcaggtgt acggtgtgcg taaagtctgg
     1381 cgtcagttgt tacgggaagg tatcagagtg gccaggtgca ctgtggcacg tctcatggcg
     1441 gttatgggac ttgccggtgt tctccggggt aaaaaggtcc gtacgaccat cagccggaaa
     1501 gccgttgccg caggcgaccg cgtaaaccgt cagttcgtgg cagaacgtcc tgaccagttg
     1561 tgggtggctg attttactta cggtgtgccc ggagttcagg gcgggcatgg atgc
//
LOCUS       DAZGJT010000268         1601 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZGJT010000268 DAZGJT010000000
VERSION     DAZGJT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.275"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            297..512
                     /gene="essD"
                     /locus_tag="ACHKFK_004940"
     CDS             297..512
                     /gene="essD"
                     /locus_tag="ACHKFK_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO9055064.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            512..709
                     /locus_tag="ACHKFK_004941"
                     /pseudo
     CDS             512..709
                     /locus_tag="ACHKFK_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            692..1601
                     /locus_tag="ACHKFK_004942"
                     /pseudo
     CDS             692..1601
                     /locus_tag="ACHKFK_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
BASE COUNT          392 a          369 c          454 g          386 t
ORIGIN      
        1 tttgttgaag ttttcttatg tatttgtggt ggtgttttga acactcggtg gcattctcac
       61 aaatatcatt tagtagttta cgtacgtaaa aaattggtta tgctgttaag agtggttact
      121 tcgtcacaca gcttaaaccc gccgtcgagc gggtttttcc attttttgag tctcgatatt
      181 agctgataac ccaatacctg agttattcac tgactccgaa tctgttacgt ttctgccttt
      241 attgcgatac gtagtatccc cttaatttac acccgctttg tctgcgaggt ggggttatga
      301 aatccatgga taagttaaca acgggtgtcg cctatggcac ctcagcaggt agtgccgggt
      361 actggttttt acagttgctc gataaagtca cgccctcaca gtgggcggca ataggtgtgc
      421 tgggtagtct ggtatttggc ttgctgacgt atctgacaaa cctttatttc aagattaaag
      481 aagataagcg taaggctgcg agaggtgaat aatgtcgcca tcattacgca aggctattgc
      541 tgctgctatt ggtggtgggg ctgttgccat agcgtctgtg ctcatcactg gtccgagtgg
      601 tgacgatggc ctggaaggtg tcagctacat accatacaaa gatattgttg gtgtatggac
      661 tgtatgtcac gggcatacag gaaaagacat catgctcggt aaaacgtata aaggcagcag
      721 taaggggcat accccgcgcg aagcgaagga caacctgaaa tccacgcagt tactgagtgt
      781 gatcgatgcc atcagcgaag ggccgattga aggtccggtg gatgggttaa aaagcgtgct
      841 actgaacagt acgccggtgc tggacactga ggggaatacc aatatctccg gtgtcacggt
      901 ggtgttccgg gcaggtgagc aggagcagac tccgccggag gggtttgaat cctccggctc
      961 cgagacggtg ctgggtacgg aagtgaaata cgacacgccg atcacccgca ccatcacgtc
     1021 ggcaaacatc gaccgtctgc gttttacctt cggtgtgcag gcactggtgg aaaccacttc
     1081 aaagggtgac cggaatccgt cggaagttcg cctgctggtt cagatacagc gtaacggtgg
     1141 ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc acctcgcagt atctggcctc
     1201 ggtggtggtg gataacctgc ctccgcgccc gttcaatatc cggatgcgca ggatgacacc
     1261 ggacagcacc acagaccagc tgcagaacaa aacgctctgg tcgtcataca ccgaaatcat
     1321 cgatatgaaa cagtgctacc cgaacacggc actggtcggt gtgcaggtgg actcggagca
     1381 gttcggcagt cagcaggtga gtcgtaatta tcatctgcgc gggcgcattc tgcaggtgcc
     1441 gtagaactat aacccgcaga cgcgacaata cagcagtatc tgggacggaa cgttaaaacc
     1501 ggcatacagc aacaacatgg cctggtgtct gtgggatatg ctgacccatc cgcgctacgg
     1561 catggggaaa cgtcttggtg cggcggatgt ggacaaatgg g
//
LOCUS       DAZGJT010000269         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZGJT010000269 DAZGJT010000000
VERSION     DAZGJT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.276"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..227)
                     /locus_tag="ACHKFK_004943"
     CDS             complement(<1..227)
                     /locus_tag="ACHKFK_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO9055065.1"
                     /translation="MFKEKLQDYTEDEFLNFLGGLRSSMKDGKSLKGKELEMYWDSLV
                     DHFIEITQHPSGSVLIFYPKSQGDDKPENIL"
     gene            complement(230..>1594)
                     /locus_tag="ACHKFK_004944"
     CDS             complement(230..>1594)
                     /locus_tag="ACHKFK_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HFO9055066.1"
                     /translation="LIANTEFDWLAFPAGYNSLFIPPKNPPASNLNPEPLPVVNLPLS
                     PPAVKPVYAKSCLKEKGCTDAGTAEEPAENFGQVAIFALPVVDDCCGYHHPEANDVGQ
                     PAEAQTMLLFPGSVLAAQIWGKWSLSGILSATCGSYIGALASALYIPSAGEGSARVPG
                     RDEFWYEEELRQKALAGSTATTRVRFFWGTDIHGKPQVYGVHTGEGTPYENVRVANMQ
                     WNEQTQRYEFTPAHDVDGPLITWTPENPEHGYVPGHTGNDRPPLEQPTILVTPIPDGT
                     DTYTTPPFPVPDPKEFNDYILVFPAGSGIKPIYVYLKEDPRKLPGVVTGRGVLLSPGT
                     RWLDMSVSNNGNGAPIPAHIADKLRGREFKTFDEFREALWLEVSQDPELIAQFSSGNQ
                     TRIKQGLTAKAPIDGWYYGPKEIVKKFQIHHRVAVEYGGSVYDIDNLRIVTPRLHDEI
                     HYRR"
BASE COUNT          390 a          410 c          373 g          421 t
ORIGIN      
        1 ttcaggatgt tttcaggctt atcatctcct tgacttttag ggtagaaaat tagaacgctc
       61 cctgacggat gctgtgtaat ttcaataaaa tgatctacta atgaatccca gtacatttct
      121 agctccttac cttttaagga ttttccatct ttcatagatg atctcaatcc gcctaaaaaa
      181 ttaagaaatt catcctcggt ataatcctgg agcttttctt taaacatatt tatctcctgt
      241 agtgaatctc atcgtgtagt ctaggggtaa caattcgtaa attatcaata tcgtatacgc
      301 tgccgccata ttcaactgca acacgatgat gtatctggaa ttttttaact atttctttag
      361 ggccataata ccaaccgtca ataggtgctt ttgcggttaa tccttgtttt atacgtgttt
      421 ggttaccact tgaaaactgc gctatcaact ccggatcctg actcacctcc agccacagcg
      481 cctcgcgaaa ctcatcaaag gttttaaact cccgtccgcg caatttatca gcaatatgcg
      541 ccgggattgg tgcgccgttg ccgttattgg ataccgacat atccagccag cgagttcctg
      601 gtgaaagcag gacgccgcgc cctgttacaa caccaggcag ctttcgcgga tcctccttca
      661 ggtaaacata gatgggctta ataccggatc ccgccggaaa aaccagaata taatcgttga
      721 attctttcgg atcaggaacc gggaatggcg gcgtggtata ggtatcggtg ccgtccggaa
      781 tcggcgtcac cagaatggtg ggctgctcca gcggcggcct gtcgttaccg gtatggcccg
      841 gaacataccc atgttccgga ttttccggcg tccaggtaat cagggggcca tcgacatcgt
      901 gagcgggggt aaattcataa cgctgcgtct gctcgttcca ctgcatgttc gccacgcgga
      961 cgttttcata cggcgtacct tcacccgtat gaacaccata cacctgaggc ttgccgtgaa
     1021 tgtcagttcc ccagaaaaaa cgtacccggg tggtggcggt actgcctgct agtgctttct
     1081 gccgcagttc ttcctcatac cagaactcat cacgtccggg cacgcgagca ctgccctcgc
     1141 ccgcagaggg aatatacaaa gcagatgcca acgcaccgat gtaagagccg caggttgcac
     1201 tgagtatgcc actgagcgac cattttcccc atatttgagc cgccaacaca ctacccggaa
     1261 acagtagcat ggtttgagct tctgcgggtt gcccaacatc gttagcctcc ggatggtggt
     1321 atccacagca gtcatcaacc actggcagag caaaaatcgc tacttgcccg aagttttcag
     1381 cgggttcttc tgccgttccg gcatctgtac atcccttctc cttcagacag gatttggcat
     1441 agaccggttt aaccgctggt ggagagagtg gaaggttaac aactggtagc ggctcagggt
     1501 taagattact ggcaggtggg tttttaggtg gaataaataa gctattatag ccagcaggaa
     1561 aggccaacca gtcaaattcc gtattggcta ttag
//
LOCUS       DAZGJT010000270         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZGJT010000270 DAZGJT010000000
VERSION     DAZGJT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.277"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1406
                     /locus_tag="ACHKFK_004945"
     CDS             <1..1406
                     /locus_tag="ACHKFK_004945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055067.1"
                     /translation="ETLRTIEPSFTNQNITVRGYYPDTPGLGGGTFIAFSSSEADDGV
                     NIFVTAGGKRWKRTGSHIDIPVENGGMMTSRTAEQNSDAFERLTACLPYEGGTLRLNG
                     FYDIKYGAIVPPRVTLEGCGMDSCGLIKTGNDIKTVPDRMWQGVAHSFSKDFIAAVDM
                     DSDTAGDLSGTQTRSTRIIGLSLKCTATGKSEYIIYSAISYNTRIEDVYGYRGMTGYY
                     TSDSWLQNVSNFMLHDVQDGYVVATAGTSFNINNLYVRDCSRHAIKMNNVTYSTLTCC
                     VPERVNGTAYVFLSCAGITLNGCAVENVTGSIFEVNQSFIVANGFRAVGVKDGGAEAI
                     IITQSNVSFNNSSLSEFEGSHTSKYYQVGGTTLNLISCYMPPASRVKWGGSFSVFNYV
                     SEGGEYSVWGTELWTATGYLVNGIAHVYGDNPPDASVTQYGNGARWELASPSAGNPYK
                     WIHVGGGVWKVAGTLAS"
BASE COUNT          472 a          269 c          391 g          462 t
ORIGIN      
        1 ttgagacact aagaactatt gagccttcat tcacaaatca aaatataaca gttcgcggat
       61 attaccctga tacaccaggt cttggtggag gaacgtttat agccttttcc agttcagagg
      121 ctgacgatgg agtaaatatc tttgtaacgg caggaggaaa acgctggaag cgaacgggaa
      181 gccatattga tatcccggta gagaacggtg gaatgatgac ctccagaacg gctgagcaaa
      241 acagtgatgc ttttgaacgg ctgactgcat gcctgccata tgaaggaggt acgctcaggc
      301 tcaatggatt ttacgatatt aagtatggcg ctatcgtgcc tccacgtgtc acgctggagg
      361 gctgtggtat ggattcctgt ggattaatca aaacaggtaa cgatattaaa acagttccgg
      421 atcgcatgtg gcagggtgtg gcgcacagtt tttccaaaga ctttatcgct gcggtagaca
      481 tggatagtga cactgctggt gatttaagcg gaacacaaac aagaagcaca agaataattg
      541 ggttgagtct aaaatgcact gcaacaggaa agtctgaata tattatatat agcgctatta
      601 gttacaatac acgaattgaa gatgtatatg gttatagagg aatgacaggg tattatacca
      661 gtgatagttg gttgcagaat gtatctaatt ttatgttgca tgatgttcag gatggatatg
      721 ttgttgcaac cgcaggaaca tcatttaata taaataatct ctatgttaga gactgctctc
      781 gccacgccat aaaaatgaat aacgtaacat atagcacatt aacatgctgc gttcctgaga
      841 gggttaatgg aactgcatat gttttcctgt catgtgctgg gattacatta aatgggtgtg
      901 cggtagagaa tgtaactggt agtatatttg aggtcaatca atcttttata gtagctaatg
      961 gattcagggc tgttggtgta aaagatggtg gcgctgaggc tataataata acgcagtcaa
     1021 atgtatcatt taacaattct tcactttctg aatttgaagg ttcacacact agtaaatact
     1081 accaagttgg tggtacaact cttaatttaa tttcatgcta tatgcctcca gcatcaagag
     1141 ttaaatgggg ggggagtttt tctgtattta attatgtaag cgagggtgga gaatactccg
     1201 tgtggggaac tgagctatgg accgcgactg gctatctagt aaatgggatt gctcatgtct
     1261 atggtgataa ccctccagat gcgagtgtca ctcaatatgg caatggagca aggtgggagc
     1321 tggcttcgcc atctgcagga aatccatata aatggataca tgtaggtggt ggagtgtgga
     1381 aggttgccgg aactttggct tcataataaa aaactgcacc caactcagtt gggtgtccaa
     1441 cttttggggt gcagtcaata aagtggtttt tattttatat actcacttat atttttacca
     1501 attgatgacc atagcgcata agccccatct ttagataagt gggtgttgtc tgaataaaga
     1561 tatttataat cgtgagacat ggttgcacat tctc
//
LOCUS       DAZGJT010000271         1570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZGJT010000271 DAZGJT010000000
VERSION     DAZGJT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.278"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..207
                     /locus_tag="ACHKFK_004946"
     CDS             7..207
                     /locus_tag="ACHKFK_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO9055068.1"
                     /translation="MEQRITLKDYAIRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEIKPFPSNKKTTA"
     gene            346..642
                     /locus_tag="ACHKFK_004947"
     CDS             346..642
                     /locus_tag="ACHKFK_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO9055069.1"
                     /translation="MEQTSYSKLSQRDIDRAETDLLINLSALTQRGLAKMIGCHESKV
                     SRTDWRYIAAILCAFQMASDISPISRAFQHAINVHANKKRPVGAGRSEQILMNI"
     gene            657..785
                     /locus_tag="ACHKFK_004948"
     CDS             657..785
                     /locus_tag="ACHKFK_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055070.1"
                     /translation="MEQYTGIILPHLEEFRQQQHQPQQPEADWVNPGDTGTVCGME"
     gene            806..>1570
                     /locus_tag="ACHKFK_004949"
     CDS             806..>1570
                     /locus_tag="ACHKFK_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO9055071.1"
                     /translation="MRNYATISPQFWLGETGRKLRKSGPECMVVALYMMTSPHSNMLG
                     LYYLPVLYIAHETGLDPEGASKGLQMACEAGFCSYDHDSEVVWVHEMAAWQVGESLKP
                     GDNRCAGVRNEYSALLENPFLSLFYDKYKDDFHLDVRRESCRKIEAPSEPLSSQEQEQ
                     EQEQERDKTLLVHGEKIATDPQGDSCPVLTERPGPAGTIPEADSGRCVQQVLLVEPEQ
                     QHQPQQPEADSAMSGKPIGLTRERPGPSAGRVDYPDV"
BASE COUNT          445 a          358 c          402 g          365 t
ORIGIN      
        1 ggttgtatgg aacaacgcat aaccctgaaa gattatgcaa tacgctttgg gcaaaccaag
       61 acggctaaag atctcggcgt atatcaaagc gcgattaaca aggccattca tgcaggccga
      121 aagatttttt taactataaa cgctgatgga agcgtttatg cggaagaaat aaagcccttc
      181 ccaagtaaca aaaaaacaac tgcataagta acaccgctat tttcacaatg gacattcgtc
      241 ctacgtcgct gacaaagcga gccccaagat atctgaccaa ctaaggccat atgcgtttcc
      301 acgcatacct ttcaactagc tattcactat tggaaatctt aagaaatgga acaaacaagt
      361 tacagcaaac tgtcacagcg cgatatcgat cgcgctgaaa cggatttact tatcaacctg
      421 tcagctctga cgcaaagggg actggcgaag atgattggct gtcatgaatc gaaggtcagt
      481 cgtaccgact ggcgatacat cgcggcgatt ttatgcgcgt ttcagatggc atctgatatc
      541 agtccgatca gccgggcttt ccagcatgcc attaacgttc atgcaaataa aaaacgtccg
      601 gttggggccg gacgttctga gcaaatcctg atgaatatct gatattcagg cagggcatgg
      661 agcaatacac gggaataatt ctgccacatc tggaagaatt tcgccagcaa caacaccaac
      721 cgcagcagcc tgaagccgat tgggttaacc cgggagatac cgggaccgtc tgcggtatgg
      781 agtaaatctt gtatgcgagg ggactatgcg taattacgca acaatttcac ctcagttctg
      841 gttaggcgaa acagggagaa aactaaggaa gtctggtccg gaatgtatgg tagtggcgtt
      901 gtatatgatg acctcgcctc attccaatat gctgggcctt tattacctgc ctgttttgta
      961 cattgctcac gaaaccggac ttgatcctga aggggcttct aaggggcttc aaatggcttg
     1021 cgaggctggt ttttgcagct atgaccatga ttctgaggtt gtatgggtgc atgaaatggc
     1081 agcatggcag gttggtgaat cgctgaaacc tggcgataac cgttgtgctg gggtaagaaa
     1141 tgaatattcc gcgttgctgg aaaatccttt tttatcatta ttttacgata aatataagga
     1201 tgatttccat ctggatgtca gacgtgagtc atgtcggaaa attgaagccc cttcagagcc
     1261 cctttcaagc caagaacagg aacaggaaca ggaacaagaa agggataaaa cccttctggt
     1321 ccatggcgaa aaaatcgcca cggacccaca gggggattct tgccctgttc tgactgaacg
     1381 accaggacca gctggcacga taccggaagc agattccggt cgttgtgtgc agcaggtgct
     1441 gctcgtcgaa ccggagcaac aacaccaacc gcagcagcct gaagccgatt ccgcgatgag
     1501 cgggaagccg attgggttaa cccgggagag accgggaccg tctgcgggac gggttgatta
     1561 tcctgacgtg
//
LOCUS       DAZGJT010000272         1546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZGJT010000272 DAZGJT010000000
VERSION     DAZGJT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.279"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(918..1394)
                     /locus_tag="ACHKFK_004950"
     CDS             complement(918..1394)
                     /locus_tag="ACHKFK_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9055072.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          351 a          415 c          385 g          395 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggcaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc cgtgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccccgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggc acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcggcg cggtcgttct ggggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc cttcagcagc
     1261 tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc caggatgcga
     1501 caacgttgtg tcaccctaga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DAZGJT010000273         1541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZGJT010000273 DAZGJT010000000
VERSION     DAZGJT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.280"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..82)
                     /locus_tag="ACHKFK_004951"
                     /pseudo
     CDS             complement(1..82)
                     /locus_tag="ACHKFK_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            235..1155
                     /gene="cbpA"
                     /locus_tag="ACHKFK_004952"
     CDS             235..1155
                     /gene="cbpA"
                     /locus_tag="ACHKFK_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO9055073.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1155..1460
                     /gene="cbpM"
                     /locus_tag="ACHKFK_004953"
     CDS             1155..1460
                     /gene="cbpM"
                     /locus_tag="ACHKFK_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO9055074.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPSEIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
BASE COUNT          388 a          379 c          416 g          358 t
ORIGIN      
        1 aatagtcgag ttgtgcattc ctttccttat tgtttaactt gtaagcatga gccaaaagtt
       61 caggagtgag gtaaataaaa tatgttcctc ccaccttgag gcgacctccc ctccgcgatg
      121 atttcaataa catattctgt gttggcatat gaaattttga ggattaccct acacttatag
      181 gagttacctt acaggggttc cttcaatttg tgttgattta cgcgagataa cgctatggaa
      241 ttaaaggatt attacgccat catgggcgtg aaaccgacgg acgatctcaa gacaatcaag
      301 accgcctatc gtcgactggc ccgcaaatac catcctgatg tcagcaaaga acctgatgcc
      361 gaagcccgct tcaaagaggt cgctgaagcc tgggaagtgt taagtgatga acaacgtcgc
      421 gctgagtatg atcagatgtg gcaacatcgc aacgatccgc aatttaaccg tcagttccat
      481 catggcgacg gtcagagttt taacgccgaa gattttgacg atatcttctc gtcaattttc
      541 ggtcagcatg cccgccagag ccgtcaacgc cccgccacac gcggccacga tattgaaatc
      601 gaagtggcgg tattcctcga agaaacgctt actgagcata agcgtaccat cagctataac
      661 ctgccggttt ataacgcctt tggcatgatc gaacaggaaa tcccgaaaac gctgaatgtg
      721 aagatcccgg cgggcgtcgg caatggtcaa cgcatccgcc tgaaaggcca ggggacgccg
      781 ggcgaaaacg gcggtccaaa tggcgacctg tggctggtga ttcacattgc gccacatccg
      841 ctgtttgata ttgtcggcca ggatctggaa atagtggtgc cggttagccc gtgggaagcg
      901 gcgctgggta ctaaagtcac cgttccaaca ctgaaagaaa gcattttgct gactatcccg
      961 cctggcagcc aggccgggca acgattgcgc gttaaaggca aaggtctggt gagcaaaaaa
     1021 cagaccggcg atctgtatgc ggtactgaaa atcgtgatgc cgccgaaacc ggatgaaaac
     1081 actgccgcgc tgtggcagca actggcagac gcccagtcgt cttttgatcc acgtaaagat
     1141 tgggggaaag cataatggct aatgttacgg tgacttttac tattaccgaa ttttgcctgc
     1201 ataccggcat ctctgaagag gagttgaatg aaattgtcgg tttgggggtg gttgaaccga
     1261 gtgagatcca ggagacaacc tgggtatttg acgaccatgc cgccattgtg gtgcaacgcg
     1321 cggtacgcct gcgtcatgaa ctggctctgg actggccggg gatcgcggtg gcgctgacgt
     1381 taatggatga tattgcgcac ctgaagcagg aaaaccgcct gctgcgccag cggctttccc
     1441 ggtttgtggc tcacccgtga gtttgttgcc tgatgcgacg cttaacgcgt cttatcaggc
     1501 ccacaaaaag cactgttccc gtaggccgga taaggcgttc a
//
LOCUS       DAZGJT010000274         1520 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZGJT010000274 DAZGJT010000000
VERSION     DAZGJT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.281"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..777
                     /locus_tag="ACHKFK_004954"
     CDS             40..777
                     /locus_tag="ACHKFK_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9055075.1"
                     /translation="MKSITKFLVCATLGLGGLTAQAHAASGDSTVSLGFAHLQLSGLN
                     NFVKDLSLHNLDTFNNFVNENYFNSPGEYDDAFVRGHDGKEKSPLGMNIKYRYEITDE
                     IGVITSFAWARSMTNAQAFIDVKPSDPSREVKKPAASARTDIRANYWSLLSGPSWRFN
                     EYLSVYSMAGVAVAKVTYDLKVNDNLNGGVGSFSESNSTKKTSIAWSVGAQFNFNESV
                     TMDVTYESSGSGDWKTDGLFAGIGLKF"
     gene            1010..1150
                     /locus_tag="ACHKFK_004955"
     CDS             1010..1150
                     /locus_tag="ACHKFK_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9055076.1"
                     /translation="MSQKSLITVTICMTVIFTIWMLHGSLCEFRLNLWGAEFAAFLQC
                     KQ"
     gene            1207..>1520
                     /locus_tag="ACHKFK_004956"
     CDS             1207..>1520
                     /locus_tag="ACHKFK_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055077.1"
                     /translation="MAPVSGERMDDKILRYMQRVVRNSRNPEFMNEVKDACLKKQAFC
                     FEAPDGFLVLRSVLSDDGIPYVLVLLGVCTGSKSVERYLPEVKTLTRLAGGRWAEFHT
                     ARR"
BASE COUNT          396 a          303 c          396 g          425 t
ORIGIN      
        1 cagagagaat aatgttttta atataggaag attcttaaaa tgaaaagtat cacaaaattt
       61 cttgtgtgcg caactctggg cttaggtgga ttaactgcac aggcacatgc tgcatctggt
      121 gatagtaccg tatcccttgg attcgctcac cttcagctat ccgggctgaa taattttgtt
      181 aaggatttaa gtcttcataa ccttgatacc tttaacaatt ttgtgaatga aaactatttc
      241 aattcacctg gagaatatga cgatgcgttt gtccggggcc atgatggtaa agagaaaagt
      301 cccttgggaa tgaacattaa gtatcgttat gagattacgg atgagatcgg tgttataacc
      361 tcttttgcat gggctcgatc catgactaat gcacaggcat tcattgatgt taagccttcc
      421 gatccatcac gagaggtaaa aaaaccggca gcttcagcga gaactgatat cagggctaat
      481 tactggagtc tactatccgg tccttcgtgg cggtttaatg aatacctcag tgtgtattca
      541 atggcaggtg ttgcggttgc aaaagtaact tatgatttga aagttaacga caatcttaat
      601 ggtggtgtag gtagtttttc tgaaagtaac agcaccaaaa aaacgtctat tgcatggtct
      661 gtcggcgcgc aatttaactt caatgaaagt gttactatgg acgtcactta tgaaagctca
      721 ggctctggcg actggaaaac tgatggttta tttgcaggca ttggcctgaa attctgagta
      781 atacccgaca acatgtcata acccgctgtg atggcgggtt ttttgttgcc cgtgcagggc
      841 aaaaatcgta gattatgcgc gggtgtcttt cggctgatgg ctggaaggtg aacctgaagg
      901 cccgatgtgg aaaggccccg agtcaaacat tttgtttaac ccgaggccct aaccatcata
      961 ccttaagcaa gtagaaggtt agcgcctctc cactcaggag gcaagcgcta tgtcgcaaaa
     1021 atcgctcatc accgtcacaa tttgtatgac ggtaatcttc accatctgga tgttacacgg
     1081 ctcgttgtgt gagttccggc tgaatttgtg gggagcggag tttgcggcgt tcttacagtg
     1141 taagcagtag gaaaaccgcg acggggacga aagttcccgt caactggttg ctgaggttca
     1201 gccgatatgg cacccgtttc tggtgagaga atggacgaca aaattctccg gtatatgcag
     1261 cgtgttgtga gaaattcccg caaccctgaa tttatgaatg aagttaaaga cgcctgcctt
     1321 aaaaagcagg cgttttgctt tgaagcgccg gatggttttc tggtgctgcg ttctgtgctc
     1381 agtgacgatg gtatccctta tgttctggtg ttactgggcg tgtgtacggg gagtaagagt
     1441 gttgagcgtt atctgccgga ggtgaagaca ttaacccgcc tggctggcgg acgctgggcc
     1501 gaatttcata cggcaagacg
//
LOCUS       DAZGJT010000275         1421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZGJT010000275 DAZGJT010000000
VERSION     DAZGJT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.282"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..334
                     /locus_tag="ACHKFK_004957"
                     /pseudo
     CDS             3..334
                     /locus_tag="ACHKFK_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            524..1090
                     /gene="slp"
                     /locus_tag="ACHKFK_004958"
     CDS             524..1090
                     /gene="slp"
                     /locus_tag="ACHKFK_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO9055078.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            1243..>1421
                     /locus_tag="ACHKFK_004959"
     CDS             1243..>1421
                     /locus_tag="ACHKFK_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO9055079.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLT"
BASE COUNT          476 a          300 c          263 g          382 t
ORIGIN      
        1 atatttataa ctgcacaaca aataccgata atgttaaaac gccagacaca ccgaagtggc
       61 cttgtgataa ccgggaagaa caacaaccca ttaattcccc attctctgga caaggataca
      121 cctctgaaca attcgattta gcgcaacaac aacgccaaca aattaatgcg tgccatacga
      181 acaccacata tacaaatgca gactactcaa agttgaggct caacttgtaa gtcttattaa
      241 taacattgaa acgataagct cgacaccact tccgcaaaag acacaatcaa tacttaatca
      301 gataaataac attagacacg aaaaaattaa aaacctctga atatagatta tggaacaatg
      361 gatactcatg ccacgcccca aaattaactg agctcaacta aacagaagag atataaacat
      421 cagacaggtt tacgttacta tcaggcatat cacctcagaa tcagatgaaa actataaaga
      481 aatatctatt atggttttaa tatttgttga taaggatagt aacatgaaca tgacaaaagg
      541 tgcactcatc ctcagccttt catttttgct tgccgcatgt agttcaattc cgcaaaatat
      601 caaaggcaat aaccaacctg atattcaaaa aagttttgtt gctgttcata accagccggg
      661 gttatatgtt ggtcaacaag cgcgctttgg tgggaaggtt atcaacgtta tcaatggcaa
      721 aacggatacg ttgttagaaa tcgctgtatt accgttggat agctatgcga agcctgatat
      781 tgaagccaac tatcagggcc gactgctcgc cagacaaagc ggcttccttg atccagtgaa
      841 ctatcgtaat cactttgtta ccatcctcgg taccattcag ggtgaacaac ctggctttat
      901 caataaagtc ccgtataact tcctggaagt gaatatgcag ggcatccagg tgtggcattt
      961 gagagaagtg gttaatacca cctataactt gtgggattac ggctatggtg cattctggcc
     1021 ggaaccgggc tggggggctc cttactacac caatgcggtg agtcaggtaa cacctgagct
     1081 ggtcaaataa caccaccgaa agatgcagat gctttctcag catctgcatc atgcattaca
     1141 tcaaattaat acacagtaag ctaactatta ttataagccc tgtcctgtta attacctttg
     1201 gcaaactgat tataaagtta atgtccgtac caggagtcgg ttatgtttct tataattacc
     1261 agggatacga tgtttttcac cgcgatgaaa aacattctga gtaaaggtaa tgtcgttcat
     1321 atacagaacg aagaagagat cgacgtaatg ttgcatcaga atgccttcgt cattattgat
     1381 acattaatga ataatgtatt tcattctaat tttctcactc a
//
LOCUS       DAZGJT010000276         1412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZGJT010000276 DAZGJT010000000
VERSION     DAZGJT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.283"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            594..819
                     /gene="ykgL"
                     /locus_tag="ACHKFK_004960"
                     /pseudo
     CDS             594..819
                     /gene="ykgL"
                     /locus_tag="ACHKFK_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(854..994)
                     /gene="ykgO"
                     /locus_tag="ACHKFK_004961"
     CDS             complement(854..994)
                     /gene="ykgO"
                     /locus_tag="ACHKFK_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO9055080.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(981..1256)
                     /gene="ykgM"
                     /locus_tag="ACHKFK_004962"
     CDS             complement(981..1256)
                     /gene="ykgM"
                     /locus_tag="ACHKFK_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO9055081.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKRGHDESP"
BASE COUNT          432 a          277 c          299 g          404 t
ORIGIN      
        1 tggttaagtc aatatttaca aaaaatgaaa atattgcttt tttgataaga agaaaaagac
       61 attcagtttc ataaggttat tggcaatata acgttcaatt cttacttgca ttttatatta
      121 gccaggaact ttgccctgag gtgttaggaa tagtcttaat gtgttgtgta atggactagt
      181 aaaaaaacaa gtttaaatga caagaaatag aatataaaac tatcataagt cataattaaa
      241 gatgatttgc caatcagtaa ggtgttttaa tggattaaca tgccgcacaa aatggttatg
      301 ggtgttcatc ccaataagtg atgaaaaatt aaacaggtgt aagtacgtca tctgtccggt
      361 aagcccccca agagccaaat taagtgaagt taaacgaaga ctgccagttg tcacaggaac
      421 gatggcctct ttgccagggc actgcattca gtcatctgtg cgcgttactc taactggcac
      481 tcgaacgtcg gcgatggggt taccgatgga tctggccgtt cctgtggcgt aaacgttttt
      541 atgtcagtac caacagattt accacattta tcatcttaac agtttgagtg taaatgcagg
      601 ctgcgtcgca aagggaggaa actgagtggc gtgttcagtc taaaagagag ttgatgccag
      661 catacagggc gaaactggtc aacaagtcaa cattaatata atggaatatt cggagagaaa
      721 cgtcaggcag ttgaccttaa atgaacagga agatacatcc ccaggaaaat taatgctggt
      781 gtaataaata caccaacatt aatccggtct gattattaat cagataaata tcgttaattc
      841 gtcgaatttt aaatcaacgt tttttcttac gaccctgaac ggccttaaaa cgtggattag
      901 atttacaaat cacatacagc cgtccttttc gcttcacaat ctgacagtct ggatggcgtt
      961 cttttgcggt acgcagagag ttaaggactt tcatcatgcc cccttttcgt gctaacaaaa
     1021 cgaccaaaac gttgggtgaa ccgtgcaaca tttccttctg atgccactgt tctcagcttc
     1081 cctgtataga acgggtgcga tttcgaagag acatcaattg ttacgtacgg atacgttacg
     1141 ccatccagct caatctcacg gtctgttttg atagtcgagc cgattttaaa gtactcatca
     1201 acgctggtgt cgtggaacac cacagtacga tactcaggat ggatattggg tttcatcatg
     1261 tttacctgtt atgttataac ataaccgtaa agatacatgc tcatccggat catttcaaca
     1321 cccaatcact gtgactattt gtgggatgtg gaatttccgc aacggggaag tctggcattg
     1381 ttagcagcca ttggcgacgc tcccataaac gc
//
LOCUS       DAZGJT010000277         1377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZGJT010000277 DAZGJT010000000
VERSION     DAZGJT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.284"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..262
                     /locus_tag="ACHKFK_004963"
     CDS             <1..262
                     /locus_tag="ACHKFK_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO9055082.1"
                     /translation="KGIVVLGDKTTHGGQVISVSSSMVINGKKVALVGDKINCPVKGH
                     GINTIIEGSFDWISDGKQVVVNGCRCECGCVVISSIADALIG"
     gene            268..1152
                     /locus_tag="ACHKFK_004964"
     CDS             268..1152
                     /locus_tag="ACHKFK_004964"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055083.1"
                     /translation="MGWKRTYTEEIDRPQDIYFHKWLFSLFILCILLLVIYYFFHEFV
                     VANNASLLAFFSPLLLWVLILSVQVIFYSRAIQDYSIWLENRLSINREWEAWGSRYVS
                     VMNSSLHLPGKVDVLFLSDDELETQYGLVNKSDDITWKQHDWYACFQTLTNHLELYKL
                     PYQLTQEFIILTDCDESMYSQIEEDFFRTVEKTNRTEGHFLLHISPSMSFGELDLWLK
                     DSEEKIYIVMVLQMEEQTSCSDAMACLMFATDDVTAKYKLTEKARIYRPMVVNSNNFN
                     SDLNILLTHKKYPKMPPG"
     gene            1179..>1377
                     /locus_tag="ACHKFK_004965"
     CDS             1179..>1377
                     /locus_tag="ACHKFK_004965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055084.1"
                     /translation="MSSNVLQCFNDNNAQLKIDNIHLLESLCGLPGGNAVWLTAALTI
                     SVVVHKNSDYLMMSENNDDWII"
BASE COUNT          402 a          203 c          305 g          467 t
ORIGIN      
        1 gaaaggtatt gttgttctgg gagataagac aacgcatggt gggcaggtta tatctgtgtc
       61 atcttcgatg gttatcaatg gaaaaaaagt tgcgcttgta ggtgataaga taaactgccc
      121 tgttaaaggg catggtatta ataccatcat cgaggggagt tttgattgga tttctgatgg
      181 caaacaggtc gtcgtgaatg gctgcaggtg tgaatgtggc tgtgttgtta tatccagtat
      241 tgctgatgcc ttgattggat gaatattatg ggatggaaac ggacgtatac agaagaaata
      301 gacaggccac aggatattta ctttcataag tggttatttt ctttatttat tttatgtata
      361 ttattactcg ttatttatta ttttttccat gaatttgttg tagctaataa cgcatctttg
      421 ttagcattct tttccccatt attattgtgg gtattgattc tttcagtaca ggtcatattt
      481 tattccagag ctattcagga ttattcaatt tggctggaga acagacttag tatcaacaga
      541 gagtgggagg catggggatc aagatatgtt tctgttatga atagtagtct tcatcttcct
      601 ggaaaagtcg atgttttatt cttgtctgat gatgaattag aaactcagta tggacttgtc
      661 aataagtcag atgatatcac atggaagcaa catgattggt atgcctgctt tcagaccctt
      721 actaatcatc ttgaacttta taaacttcca tatcaactta cacaagaatt tattattctt
      781 actgattgtg atgaaagtat gtactctcag attgaagagg actttttcag aacagtggag
      841 aaaaccaaca gaacagaggg gcattttttg ttgcatattt ccccttcaat gtcctttgga
      901 gagcttgatt tatggttaaa ggattccgaa gagaaaatat atattgtcat ggtgttgcag
      961 atggaggagc aaacatcctg ttcggacgct atggcttgcc ttatgtttgc aactgatgat
     1021 gttactgcta agtacaaact cactgaaaag gccagaattt atcgcccaat ggtagtaaat
     1081 agtaataatt tcaatagtga cttgaatatt ttattgacac acaaaaaata tccaaaaatg
     1141 ccgccgggtt agttggggac agtcagcgtc tgttttctgt gagtagtaat gtattgcagt
     1201 gttttaatga taataatgct caattgaaaa ttgataatat ccatctttta gaaagccttt
     1261 gcggattacc agggggaaat gcggtctggt taacggcagc gctgactatt tcggtggttg
     1321 ttcacaaaaa ttcagattac ttgatgatgt ccgaaaataa tgatgactgg atcatta
//
LOCUS       DAZGJT010000278         1352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZGJT010000278 DAZGJT010000000
VERSION     DAZGJT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.285"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..380
                     /locus_tag="ACHKFK_004966"
     CDS             <1..380
                     /locus_tag="ACHKFK_004966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO9055085.1"
                     /translation="ITYKVQRDKKARELELANATITDMQVRQRDVAALDAKYSRELAD
                     ARAENETLRADVAAGRKRLRINATCSGTVREATGTSGMDNATGPRLADTAERDYFTLR
                     ERLMMMQKQLEGAQEYIRTQCLK"
     gene            682..>1352
                     /locus_tag="ACHKFK_004967"
     CDS             682..>1352
                     /locus_tag="ACHKFK_004967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9055086.1"
                     /translation="MAKLDWKKLEQAFRREHARTGIKLQDWCRQNNICYGTARRYIKL
                     RKNSPKNTEESAQKNAQKSAQIDTQKSAQKNAHGADDESSNDESYGDDGSDEKCAKNC
                     ANSAETKRNSRSGNPHPVARFSDRNTHAVRHRGYAKYLEADNLMDDASDMVLFDELVF
                     TRARALSVTGTLKKMFADLKEAADVETRVALYDKILKAEQALDRNIARIESIERSLLT
                     LDVLA"
BASE COUNT          396 a          337 c          357 g          262 t
ORIGIN      
        1 ccatcaccta caaagtgcag cgcgataaaa aagccagaga gctggagctg gcaaacgcca
       61 ccattactga tatgcaggtg cgccagcgcg atgttgctgc gctcgatgca aaatactcga
      121 gggaattagc cgatgcgaga gctgaaaatg aaactctgcg tgctgatgtt gctgctggtc
      181 gtaagcgcct gcggatcaac gccacctgct ccggtaccgt gcgtgaagcc accggcacct
      241 ccggcatgga taatgcaacc ggcccccgac tggcagacac cgctgaacgg gattatttca
      301 ccctcagaga gcggttgatg atgatgcaga agcagctgga aggggcacag gaatatatcc
      361 gcactcagtg cctgaaataa gttttgctga tgcgccgtat cgtcgcagta ttcccgcatt
      421 aacagagacc tcagccagac actggacctc ctgcgcgagt gtgtggggat aatcaaaaac
      481 gatgcacacc gggctttcca cgttttcagg taacgtgagc gttttcccgc atggccgcct
      541 gtccggtgcg gtggtggaag aaaccggata aaacaaccat attgtgtaaa tatcaatcaa
      601 acaagacgct gttgtgtgaa ttctgaaaaa tcacagcggt cattatgcgt cagtttttaa
      661 cagaggacgt cagaaagtga catggcaaag ctggactgga aaaaactgga gcaggcattc
      721 cgacgcgaac atgccagaac tggcataaaa cttcaggact ggtgtcgaca gaacaatatc
      781 tgctatggca cggctcgtcg gtacataaaa ctgcgcaaaa actcccccaa aaatacagag
      841 gaaagtgcgc agaaaaatgc gcaaaaaagt gcgcaaattg acactcaaaa aagcgcacaa
      901 aaaaatgcgc atggagctga tgacgagtcc agtaatgacg agtcttacgg cgatgatggc
      961 agtgatgaaa aatgcgcaaa aaactgcgca aattcggcag aaacgaaacg caacagcaga
     1021 tcgggcaatc ctcaccccgt tgcccggttt agcgaccgta acacccacgc cgtgagacac
     1081 cgtggatatg cgaagtatct tgaggcagat aacctcatgg atgatgcgtc cgatatggtg
     1141 ctgttcgatg aactggtgtt cacccgtgcc cgcgcacttt cagtaacagg aacactgaaa
     1201 aaaatgttcg ccgacctgaa agaggcggct gacgtggaaa cccgtgttgc cctgtacgac
     1261 aaaatactca aagcagaaca ggcccttgac cggaatattg cccgtatcga gtcaattgaa
     1321 cgctcattgc tgacgctgga cgttctggcg ga
//
LOCUS       DAZGJT010000279         1313 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZGJT010000279 DAZGJT010000000
VERSION     DAZGJT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.286"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1313
                     /locus_tag="ACHKFK_004968"
     CDS             <1..>1313
                     /locus_tag="ACHKFK_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFO9055087.1"
                     /translation="LKQREQDSDRYSGREDDPQVPRQLRQSRHRRPLPAHLPREIHRL
                     EPEESCCPECGGELDYLGEVSAEQLELVSSALKVIRTERVKKACTKCDCIVEAPAPSR
                     PIERGIAGPGLLARVLTGKYCEHLPLYRQSEIFARQGVELSRALLSNWVDACCQLMTP
                     VNDALYRYVMNTRKVHTDDTPVKVLAPGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFA
                     YSPNRQGKHPEQHLRPFRGILQADAFTGYDRLFSAEREGGALTEVACWAHARRKIHDV
                     YISSKSATAEEALKRISELYAIEDEIRGLPESERLAVRQQRSKVLLTSLHEWMVEKNG
                     TLSKKSRLGEAFSYVLNQWDALCYYSDDGLAEADNNAAERALRAVCLGKKNFMFFGSD
                     HGGERGALLYGLIGTCRLNGIDPEAYLRHILSVLPEWPSNRVDELL"
BASE COUNT          319 a          332 c          397 g          265 t
ORIGIN      
        1 ctgaaacaac gtgaacagga cagcgatcgt tacagtgggc gggaagacga tcctcaggtt
       61 ccccgccagt tgcgacagtc gcgccatcgt cgtccgttac cggcacacct tccccgtgaa
      121 atacaccgcc tggagccaga agaaagctgt tgcccggagt gtggcggtga gctggattat
      181 ctgggggaag tcagcgctga acagctggaa ctggtgagca gtgccctgaa agtgatccgc
      241 acagaacggg taaaaaaagc ctgtacaaaa tgtgactgta ttgttgaagc accggcgccg
      301 tcccgcccga tagagcgtgg tatcgcgggc cccggattac ttgcccgcgt gttaacggga
      361 aaatactgcg aacatctgcc actgtatcgt cagagtgaaa tctttgcccg ccagggtgtc
      421 gaactgagcc gggccttact ctccaactgg gttgacgcgt gctgccagtt aatgacaccg
      481 gtgaatgatg ccctgtaccg ttatgtaatg aacacccgca aggttcacac tgatgacaca
      541 ccggtaaagg tactggcacc gggtcagaaa aaggcgaaaa cagggcgtat ctggacgtat
      601 gtccgggatg atcgcaatgt gggttcgtca tctcctccag cggtctggtt cgcgtactcg
      661 ccgaaccggc aggggaaaca cccggagcaa cacctccgcc ccttccgggg tatcctgcag
      721 gcggatgcgt tcacaggtta cgacaggttg ttcagtgcag aacgtgaagg tggtgcactg
      781 acagaagttg cgtgctgggc ccatgcccgg cgaaaaatcc acgatgtata catcagcagc
      841 aaaagtgcga cggcagaaga agccctgaag cgaatcagtg aactgtacgc catcgaggat
      901 gaaatacggg gattaccgga gtcagagcgt cttgccgtca ggcagcagcg aagcaaagtg
      961 ttactgacgt cgctgcatga atggatggtg gagaagaatg gtacgctgtc gaaaaaatcc
     1021 agactgggcg aagcgttcag ctatgtactg aatcagtggg atgccctctg ttattacagt
     1081 gatgacggtc tggcggaggc ggataataat gctgcggaaa gagcgcttcg tgcagtctgt
     1141 ctcggaaaga aaaactttat gttctttggc agcgatcacg gcggcgagcg tggagcactg
     1201 ttgtacgggc tgatcggcac ctgccgtctg aacggtatcg atccggaagc gtatctgcgc
     1261 catatcctga gcgtactgcc ggaatggcct tccaaccgag ttgatgaact cct
//
LOCUS       DAZGJT010000280         1291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZGJT010000280 DAZGJT010000000
VERSION     DAZGJT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.287"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..1144
                     /locus_tag="ACHKFK_004969"
     CDS             32..1144
                     /locus_tag="ACHKFK_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS421 family transposase"
                     /protein_id="HFO9055088.1"
                     /translation="MNYSHDNWSAILAHIGKPEELDTSARNAGALTRRREIRDAATLL
                     RLGLAYGPGGMSLREVTAWAQLHDVATLSDVALLKRLRNAADWFGILAAQTLAVRAAV
                     TGCTSGKRLRLVDGTAISAPGGGSAEWRLHMGYDPHTCQFTDFELTDSRDAERLDRFA
                     QTADEIRIADRGFGSRPECIRSLAFGEADYIVRVHWRGLRWLTAEGMRFDMMGFLRGL
                     DCGKNGETTVMIGNSGNKKAGAPFPARLIAVSLPPEKALISKTRLLSENRRKGRVVQA
                     ETLEAAGHVLLLTSLPEDEYSAEQVADCYRLRWQIELAFKRLKSLLHLDALRAKEPEL
                     AKAWIFANLLAAFLIDDIIQPSLDFPPRSAGSEKKN"
BASE COUNT          324 a          314 c          351 g          302 t
ORIGIN      
        1 ggtattacgc ctgatatgat ttaacgtgcc gatgaattac tctcacgata actggtcagc
       61 aattctggcc catattggta agcccgaaga actggatact tcggcacgta atgccggggc
      121 tctaacccgc cgccgcgaaa ttcgtgatgc tgcaactctg ctacgtctgg ggctggctta
      181 cggccccggg gggatgtcat tacgtgaagt cactgcatgg gctcagctcc atgacgttgc
      241 aacattatct gacgtggctc tcctgaagcg gctgcggaat gccgccgact ggtttggcat
      301 acttgccgca caaacacttg ctgtacgcgc cgcagttacg ggttgtacaa gcggaaagag
      361 attgcgtctt gtcgatggaa cagcaatcag tgcgcccggg ggcggcagcg ctgaatggcg
      421 actacatatg ggatatgatc ctcatacctg tcagttcact gattttgagc taaccgacag
      481 cagagacgct gaacggctgg accgatttgc gcaaacggca gacgagatac gcattgctga
      541 ccggggattc ggttcgcgtc ccgaatgtat ccgctcactt gcttttggag aagctgatta
      601 tatcgtccgg gttcactggc gaggattgcg ctggttaact gcagaaggaa tgcgctttga
      661 catgatgggt tttctgcgcg ggctggattg cggtaagaac ggtgaaacca ctgtaatgat
      721 aggcaattca ggtaataaaa aagccggagc tccctttccg gcacgtctca ttgccgtatc
      781 acttcctccc gaaaaagcat taatcagtaa aacccgactg ctcagcgaga atcgtcgaaa
      841 aggacgagta gttcaggcgg aaacgctgga agcagcgggc catgtgctat tgctaacatc
      901 attaccggaa gatgaatatt cagcagagca agtggctgat tgttaccgtc tgcgatggca
      961 aattgaactg gcttttaagc ggctcaaaag tttgctgcac ctggatgctt tgcgtgcaaa
     1021 ggaacctgaa ctcgcgaaag catggatatt tgctaatcta ctcgccgcat ttttaattga
     1081 cgacataatc cagccatcgc tggatttccc ccccagaagt gccggatccg aaaagaagaa
     1141 ctaactcgtt gtggagaata acaaaaatgg tcatctggag cttacaggtg gccattcgtg
     1201 ggacagtatc cctgacagcc tacaaaacgc aattgaagaa cgcgaggcat cgtcttaacg
     1261 aggcaccgag gcgtcgcatt cttcagatgg t
//
LOCUS       DAZGJT010000281         1288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZGJT010000281 DAZGJT010000000
VERSION     DAZGJT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.288"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            153..743
                     /locus_tag="ACHKFK_004970"
     CDS             153..743
                     /locus_tag="ACHKFK_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055089.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPPVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVRREGDPLEVAQWLQACHDAGMEVRVQINESAAP"
     gene            complement(763..1110)
                     /locus_tag="ACHKFK_004971"
     CDS             complement(763..1110)
                     /locus_tag="ACHKFK_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
                     /protein_id="HFO9055090.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
BASE COUNT          311 a          349 c          382 g          246 t
ORIGIN      
        1 tgcgagtcag ccaaagccgg agccgtgctg atcttctggc atgaactggc cataaatggc
       61 tatacctcaa tgaatgccat aaagagacag gagatcattg acgccgacca tcagcgcctc
      121 agaaagctca tctggccgga ggatgactgg aaatgactca gagccgccga ccgtcgccac
      181 tgcagcggag agtgctgatt gtgctggctg cccttgatga aaaacggcca ggacccgtgc
      241 tgacgcggga catagaacgg gtgctggagc aaagcgggga agccccggtc tacgggccaa
      301 acctgcgcgc ctcctgccgt cgtctggagg atgccggctg gctgcgtacc ctgcgcgctc
      361 cgaacctgca gctggcagtg gagctgacgg acgccggccg ggccgtggca cagcctctgc
      421 tgctggcaga acaggaccgc atccgggcag aacagcgtgc ggcagaagtc gtcgtactgc
      481 cgccggtccc ggcagcaggt ctgcccgcag gcggcacttc tgccactgat ctggcggtgg
      541 agctgaacgg cataacgtat caggcctgcc gtggggattt tgtggtgcgt ctcgacggca
      601 gcacctgtct gcagttatgg aataaagaag gcagggtggt acgccgggag ggcgatccgc
      661 tggaagtggc gcagtggctg caggcctgtc acgatgcagg aatggaggtt cgcgtacaga
      721 taaatgaaag tgccgctccg taagaacacg cctgtgcacg gtttacacat catcccatgg
      781 tggcagccgg ttaactttcc tgcgccacca gtggaaagag gccatgagca gtccggacaa
      841 aaaacccccc gtgatactta taataatggc ctcacctgcc accattcctg acggccccca
      901 gtagataaac cacattgcac atccccagga aatgccccat aagccccccg tcagcaccgt
      961 gacctgccag aacggcataa agggtaatgg cggaagccgg atacccagcc gccagagaat
     1021 acgcagaaga ggaggggcat aattgctccg ccacatcttt ttgctgtcca tcagggcaat
     1081 ggcccgggct tttttctgct caaaagtcac aggagcacct ccgttccatg atgtaaacgt
     1141 gacacagctt attgttattg attattatta tcatgctgtg gttatgttgt catgcatcag
     1201 tacactgaca aaagtgcaaa agaaaggaaa actggcagat gaacagaaaa tattatttta
     1261 ataatatgtg gtggggatgg gtgacggg
//
LOCUS       DAZGJT010000282         1275 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZGJT010000282 DAZGJT010000000
VERSION     DAZGJT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.289"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(239..>1275)
                     /locus_tag="ACHKFK_004972"
     CDS             complement(239..>1275)
                     /locus_tag="ACHKFK_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III restriction-modification system
                     endonuclease"
                     /protein_id="HFO9055091.1"
                     /translation="DAYSRLKAKYPAAFPTGVKPSKIKKATDGKRRTKMRVGKFSELK
                     ELWDLINQKAVIEYKINSENEFLSIFKSFMLEETERFTKSGVHTRIDKIYIYNDTAMS
                     KSIISGDDNFAKLNTMSYREFLDNLSQTIFAKHDTLHKVFCDIKETINITEYLNIQTI
                     RKIKSGFSKYLLNNSFNKFSLGYNVISGSIHPTKFTNADGEPVNEVLSSDLGILNDNT
                     KPPLDSYLFEEVFYDSELERLNITDGEIQSVAVFTKIPKNSIKIPVAGGYTYSPDFAY
                     IVKTIKGDYLNFIIETKNVDGKDSLRLEEERKIKHAQALFSQISKSVKVEFRTQFSTD
                     RIAEMIKQVG"
BASE COUNT          368 a          228 c          217 g          462 t
ORIGIN      
        1 aagagacaag gcgtcaggaa cttattttac cctccacatt ttattgtgga agtttgaatt
       61 tattcttggt cggtagttat gattatggtc tgattgcccc atacatgaga agtagttaac
      121 agcccaatga gctgattgat ttaccccaag tgtggggata tgtattctat ctaatggaaa
      181 gttcccttta aaggaaaaca aaagggccgt aagacccttt tccatttaca acatattatc
      241 atccaacttg tttaatcatt tcagctattc tgtctgtaga aaattgtgtt ctgaactcga
      301 ctttaacaga tttacttatt tgactaaata atgcttgcgc atgttttatc ttacgttctt
      361 cttcaagtct taagctatcc tttccatcaa cattttttgt ttcgataata aagtttaaat
      421 aatcaccttt tattgttttg acaatatatg caaagtcagg ggagtacgtg tatccacctg
      481 ccaccgggat cttaatggag ttcttcggta tctttgtaaa aacagctaca gactggatct
      541 ctccatctgt tatgttgagc cgttcaagct ctgagtcata aaagacttcc tcaaaaagat
      601 aagaatccaa gggtggtttt gtgttgtcat tcaatatgcc aagatcactt gataatacct
      661 cattcacagg ctcaccgtct gcattagtga actttgtagg atgaattgaa cctgagatca
      721 cattatatcc aagactgaat ttattaaacg aattgttcaa caaatacttg ctaaaaccag
      781 atttaatctt tctgattgtt tgaatgttca ggtattcagt gatattaata gtttctttta
      841 tatcacaaaa aaccttatgt aaggtatcgt gtttagcaaa aatagtttgt gataagttat
      901 caagaaattc ccgatagctc attgtgttta gtttagcaaa gttatcatca ccactgataa
      961 tgcttttcga cattgccgta tcattataga tataaatttt atcaatgcga gtatgaacgc
     1021 ctgatttagt aaatctttct gtttcttcga gcatgaatga cttgaaaatg gataagaatt
     1081 cattttcgct atttatttta tactcaataa ctgctttttg attgatcaaa tcccatagtt
     1141 ctttcagctc actgaactta ccaacacgca ttttagtgcg tctttttcca tcagtggctt
     1201 ttttgatttt gcttggtttc actcctgtag gaaatgctgc cggatatttg gcttttaagc
     1261 ggctataggc atctg
//
LOCUS       DAZGJT010000283         1264 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZGJT010000283 DAZGJT010000000
VERSION     DAZGJT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.290"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            184..464
                     /locus_tag="ACHKFK_004973"
                     /pseudo
     CDS             184..464
                     /locus_tag="ACHKFK_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element ISEc30 family transposase"
     gene            complement(517..1221)
                     /locus_tag="ACHKFK_004974"
     CDS             complement(517..1221)
                     /locus_tag="ACHKFK_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020324562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HFO9055092.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIIGATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
BASE COUNT          311 a          300 c          304 g          349 t
ORIGIN      
        1 atattccgtt tttttagata aatataacca gaaaaaatca ttgttgattt tatgcgttta
       61 ttatttgatt ataagttgtg attattataa tttagttatt gttagtatgt tatataatgc
      121 tggataggtg atgcctctaa ttagttgaat ctgatgtata atacgggttt ttgaggttat
      181 ctcatggcca gagttaatat tcattgtccc cgttgtcagt cagctcaggt ttaccgccat
      241 ggtcagaacc ctaaaggccg tgacagactt cgctgccgtg actgccaccg tgtgtttcag
      301 ctcacttata cttatcaagc acgtaagccg ggtatgaaag agctgatcac tgaaatggcc
      361 tttaatggtg ccggggttcg cgataccgcc aggacactga aaattggtag taacaccgtc
      421 atccggactt taaaaaactc gcgccaaagc gaataacttc ttcgggcact gttgcaaagt
      481 tagcgatgag gcagcctttt gtcttattca aaggccttac atttcaaaaa ctctgcttac
      541 caggcgcatt tcgcccaggg gatcaccata ataaaatgct gaggcctggc ctttgcgtag
      601 tgcacgcatc acctcaatac ctttgatggt ggcgtaagcc gtcttcatgg atttaaatcc
      661 cagcgtggcg ccgattatcc gtttcagttt gccatgatcg cattcaatca cgttgttccg
      721 gtacttaatc tgtcggtgtt caacgtcaga cgggcaccgg ccttcgcgtt tgagcagagc
      781 aagcgcgcga ccataggcgg gcgctttatc cgtgttgatg aatcgcggga tctgccactt
      841 cttcacgttg ttgaggattt tacccagaaa ccggtatgca gctttgctgt tacgacggga
      901 ggagagataa aaatcgacag tgcggccccg gctgtcgacg gcccggtaca gatacgccca
      961 gcggccattg accttcacgt aggtttcatc catgtgccac gggcaaagat cggaagggtt
     1021 acgccagtac cagcgcagcc gtttttccat ttcaggcgca taacgctgaa cccagcggta
     1081 aatcgtggag tgatcgacat tcactccgcg ttcagccagc atctcctgca gctcacggta
     1141 actgatgccg tatttgcagt accagcgtac ggcccacaga atgatgtcac gctgaaaatg
     1201 ccggcctttg aatgggttca tgtgcagctc catcagcaaa aagggatgat aagtttatca
     1261 ccac
//
LOCUS       DAZGJT010000284         1256 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZGJT010000284 DAZGJT010000000
VERSION     DAZGJT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1256
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.291"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..646
                     /locus_tag="ACHKFK_004975"
     CDS             143..646
                     /locus_tag="ACHKFK_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO9055093.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(725..976)
                     /gene="yecJ"
                     /locus_tag="ACHKFK_004976"
     CDS             complement(725..976)
                     /gene="yecJ"
                     /locus_tag="ACHKFK_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO9055094.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1091..1177)
                     /gene="azuC"
                     /locus_tag="ACHKFK_004977"
     CDS             complement(1091..1177)
                     /gene="azuC"
                     /locus_tag="ACHKFK_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO9055095.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
BASE COUNT          336 a          292 c          280 g          348 t
ORIGIN      
        1 gttattctta tttcagatca tcgtcagaat tgactccacg atcacatttc ggaccggcag
       61 aaaggaatta ttctgcaaac agtaattatg gtgttttgat ttatcttgca cctctccact
      121 tctggatata aggatattag gtatggcaac cgctggaatg cttctcaaac tcaactctca
      181 aatgaaccgc gagttttacg catccaatct ctaccttcac ctgagtaact ggtgttctga
      241 acagagtctg aacggcaccg ccactttcct tcgcgcccag gcacagagta atgtgaccca
      301 aatgatgcgc atgtttaact ttatgaagag tgtcggcgct acccccatcg ttaaagccat
      361 tgatgttccc ggtgaaaaac tgaactctct ggaagaactg ttccaaaaaa cgatggaaga
      421 atacgagcaa cgttcaagca cgctggcgca gttagccgat gaagcgaaag aactgaatga
      481 tgattcaacc gtcaatttcc tgcgcgatct ggaaaaagaa cagcagcatg atggcctgtt
      541 actgcaaacc attcttgatg aagtgcgcag tgcgaaactt gctggtatgt gtcctgtgca
      601 gaccgaccaa catgttctga atgtcgtgtc gcatcagctg cattgatcat acatcggcgc
      661 caatgcattg cgccgatgaa gtttttgaga aaccgctgcc tcattttttt gaagcagcgg
      721 ttttttaatg ggattcgccc tgtggagtaa acttgagttc aatcaacgcg atggcttttt
      781 ggattgcccg catggtgacc gggtctgcgg cggcgggatg gttagtaaag tcgatattct
      841 tcagctggct ggacattttt tcgcgaactt caacgggcgc gatcacatcg agaacatcca
      901 gaatttgttt gataaccaac tggcaagcaa ccacatcaga aaccagttcc tgatcggcat
      961 tcagcggctg ggacatcgta aactcctgat agcattttga aagccgttat agtagcggct
     1021 tcacatcttc agcgatagtc acatccaccg tcatcagaac acaaaaaaac ctgccggagc
     1081 aggttttttg ttatcggaac atattgcctg gcggtacgtc tttgaacgtc ttgcaatagt
     1141 tattgaacat acttttcagg atcttgcgca gtttcatcgc ggtactccga ccatttgtta
     1201 tacaggtgtt attgtctttg cgtttataat atgacaacca tcacaaaaat caatct
//
LOCUS       DAZGJT010000285         1251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZGJT010000285 DAZGJT010000000
VERSION     DAZGJT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.292"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..1000)
                     /gene="leuO"
                     /locus_tag="ACHKFK_004978"
     CDS             complement(56..1000)
                     /gene="leuO"
                     /locus_tag="ACHKFK_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO9055096.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     ALGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQTWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          380 a          285 c          252 g          334 t
ORIGIN      
        1 caataaaaaa tgcagaataa accagacatt gatgtctgac ctattctgca ttcagttagc
       61 gtttgcaaat tgagactaat tgctcttcca tccactgatg gcctttatcg cgcccggcag
      121 cttcatgcca ggagagataa caggttctgc tgttttgttt taacggcagc ggtaatacct
      181 gtaactctaa ggattcagcg aactcttcag ccaaccaacg cggcgcaata gcgaccagat
      241 gcgtttgcga caccacgcta agtacgctca tcattgccat gccctgatac gcgatactgg
      301 cttgcttatc taccgtgtca taccaggttt gactaaatga tgcgaaacga tcgagcgaaa
      361 ccgcagcgtg ttgttcgtta taaacatcat gcttcagtaa cgggccttta attgttggat
      421 gatttttgct ggctaccaac accatttcat ctttaaataa tggcacactg gtaaattcag
      481 ggcgatggaa gtcttcataa ctaatcacaa actccgtttc ctgataacgc agctgatgtt
      541 cagtgttctg atttaatgaa gacttgaaca taacatgtat atttggcgca atctgctcaa
      601 tgtgattata aatctgcgag gtcagaatgc tgtctaacgg gctgcaaaca caaagatgaa
      661 atacacgttc actgctcgcg ggttcaaaac ctgaaccagg caattcattt tgtactagtt
      721 gcaatgcctg acgaactgag ccaaaaagtt gaaatgcgcg agcagtcggt tgaataccac
      781 ggccataacg aacaaaaagc tcgtcattaa acatcacctt caggcgtgca acagcgttac
      841 tgaccgcagg ttgcgacatt cccagagcat gagcggcacg ggtgatgttt tgctcctgca
      901 tcacggcatc gaaaacggtt aataagttga gatcgaccat gcgtagctgt ggcttgctta
      961 actccgccgt ctctggatga tctgtttgta cctctggcat acttaactcc actgtcacac
     1021 ttaactccct ttcccttatt ggaatgcaga gaaataatcc tcgagaatat gatttatcat
     1081 gcatataaaa taagaaaaag caaaatgagt aaaattagga aaacataaag atacaggttc
     1141 tgataaaaac agaatccact gccgagcaaa gagtcattta ctacagcata ataatccata
     1201 atgtttttgt tgcgaaaaca atctaatcat aactaccacg aatactcaat c
//
LOCUS       DAZGJT010000286         1233 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZGJT010000286 DAZGJT010000000
VERSION     DAZGJT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.293"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            263..>1233
                     /gene="aslA"
                     /locus_tag="ACHKFK_004979"
     CDS             263..>1233
                     /gene="aslA"
                     /locus_tag="ACHKFK_004979"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase AslA"
                     /protein_id="HFO9055097.1"
                     /translation="MEFSFSPKLLVVAVAAALPLIASAADTPSTATARKGFAGYDHPN
                     QYLVKPATTIADNMMPVMQHPAQDKETQQKLAELEKKTGKKPNVVVFLLDDVGWMDVG
                     FNGGGVAVGNPTPDIDAVASQGLILTSAYSQPSSSPTRATILTGQYSIHHGILMPPMY
                     GQPGGLQGLTTLPQLLHDQGYVTQAIGKWHMGENKESQPQNVGFDDFRGFNSVSDMYT
                     EWRDVHVNPEVALSPDRSEYIQKLPFSKDDVHAVRGGEQEAIADITPKYMEDLDQRWM
                     EYGVKFLDKMAKSDKPFFLYYGTRGCHFDNYPNAKYAGSSPARTSYG"
BASE COUNT          328 a          321 c          286 g          298 t
ORIGIN      
        1 ttaataacat atatattaat tgccgttaaa actaaaaaca gcatcaataa tcaacgcgat
       61 ataacatcac cgccttacat atcacctgcg ccagaggtag gattgcaaac gctctcctga
      121 ttttccaatt cattttctgg ataaataaat aatttatttt tatcactatt atttatgcaa
      181 tcatcctgtc agggagaggg atctcaatta tcaatgctta attacgtcat cattttgacg
      241 acatgaaacc ggagcatttt gcatggaatt ttcgttttca cccaaacttc ttgttgttgc
      301 tgtcgccgca gctcttcctc tcatagccag cgcagcagat accccgtcaa ccgccaccgc
      361 acgcaaaggc tttgccggat acgatcaccc aaaccagtat ctggttaaac cggcgaccac
      421 tattgccgac aacatgatgc cggtgatgca gcatcctgca caagacaaag aaacccagca
      481 gaaactggcg gaacttgaga aaaaaaccgg taagaaaccg aacgtggttg ttttcttgct
      541 ggatgatgtg ggctggatgg acgttggctt taacggcggc ggcgtggcgg tgggtaaccc
      601 tacaccagat atcgacgccg ttgccagcca ggggctgatt ttaacttcgg catattctca
      661 accgagttct tccccaaccc gcgccacgat tctcaccgga caatactcca tccaccacgg
      721 cattctgatg ccgccaatgt acgggcaacc gggcggtctg caagggttaa ccacgctgcc
      781 gcagttgctg cacgatcagg gctacgtcac ccaggctatc gggaaatggc atatggggga
      841 aaacaaagag tcgcagccgc agaacgttgg ctttgatgat ttccgcggct ttaactcggt
      901 atctgatatg tataccgaat ggcgtgatgt tcatgtcaac ccggaagtcg ccctgagccc
      961 ggatcgttct gaatacatcc aaaaactgcc gttcagcaaa gatgacgttc atgccgtgcg
     1021 cggcggtgaa caggaagcca ttgccgacat tacgccgaaa tatatggaag atctggatca
     1081 acgctggatg gaatatggcg ttaagttcct cgacaagatg gcgaagagtg ataagccttt
     1141 cttcctctat tacggcactc gcggctgtca cttcgataac tacccaaatg cgaaatatgc
     1201 gggtagctct ccggcgcgca cctcttacgg cga
//
LOCUS       DAZGJT010000287         1226 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZGJT010000287 DAZGJT010000000
VERSION     DAZGJT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1226
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.294"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..92
                     /locus_tag="ACHKFK_004980"
     CDS             <1..92
                     /locus_tag="ACHKFK_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055098.1"
                     /translation="YFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
     gene            complement(127..>1226)
                     /locus_tag="ACHKFK_004981"
     CDS             complement(127..>1226)
                     /locus_tag="ACHKFK_004981"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO9055099.1"
                     /translation="PPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGYSNYTFVNDNCS
                     IHRVGFSRTYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVHNSMKLYSQIRK
                     RAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIEIVPNGIDLETY
                     QSTFQPITRSELNISAEEKIIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDY
                     TEMSKSDKGAYQRNVREIANRLKDRCIMLGCIPPEKMHCYYPLADLVVIPSQFQEPFC
                     MVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTA
                     VAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          377 a          248 c          210 g          391 t
ORIGIN      
        1 gttattttgt tatccgtgga aacgtagaac aaattgaacc aaatttatta ggcgtttacg
       61 ccggcttatt attagcgatg aaaaacaagt aaaaaataaa aaggctgcat aatgcggcct
      121 ttttatttat tcaaaccaat tatgaataac ctcttcaaat ctttgagtta cgccatccca
      181 actgtagtga tcaaacacaa aatcctgacc ttgctttgca actgctgtta attcaggatt
      241 cgctaacgtt tttaaaatat cgctgcttat agagtctgca gtcataggtt cttttaaatg
      301 gaatcccgta gtattttctt tgacaaattc cgtcatcccc ccacgcgtac tcactaaaac
      361 aggcttacca gcccccatag cttcaattgc caccatgcaa aatggttcct ggaactggga
      421 aggaatgacg actaaatctg ccagagggta ataacaatgc atcttttcag gcggtatgca
      481 tcctaacata atgcaacgat ccttcaaacg gtttgcaatt tccctgacat tgcgttgata
      541 tgcacctttg tcacttttac tcatttctgt ataatcacca atcacaacta attttaaatt
      601 tttgtgagca gctgccaatt tttcgaatga ttgcatcaac agtagtatgc ctttgtcagg
      661 gacgatacgc ccagcataaa aaataatttt ctcttcagcg gagatattaa gttcgcttct
      721 agtgatcggc tgaaaagtag attgataggt ttctaaatca attccatttg gtacaatctc
      781 aatatctgca tttgctaaat aagattggta atactttttc aagtataaac taggcactat
      841 cattttaaca ttttgctcaa gcaattttgg ttcaaatgca ttatgcatat gtataaccac
      901 tcttgcctga ggtgctcgct tacgaatttg agaatatagc ttcatgctat tatgaactac
      961 aataacactt tctttcgtta taggaaaatc atgtgcaata ttcaatattc tctgtgaata
     1021 tggaagagga tcaagtcttg tccatttttg aaataaccgc ttatatgtgc ggctgaatcc
     1081 aaccctatga atactacaat tatcattaac aaaggtataa tttgagtatc ctggattctt
     1141 gatacaaaca atacgatttg ggaaattggt acgctgagca acctggtaca tccacgtttc
     1201 aacagcggcc gctccgcgag gaggaa
//
LOCUS       DAZGJT010000288         1222 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZGJT010000288 DAZGJT010000000
VERSION     DAZGJT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1222
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.295"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(35..118)
                     /locus_tag="ACHKFK_004982"
     CDS             complement(35..118)
                     /locus_tag="ACHKFK_004982"
                     /inference="COORDINATES: protein motif:HMM:NF013968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO9055100.1"
                     /translation="MICITVVLIIWITHRSLCEFRFRIAPF"
     gene            complement(335..>1222)
                     /locus_tag="ACHKFK_004983"
     CDS             complement(335..>1222)
                     /locus_tag="ACHKFK_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9055101.1"
                     /translation="NDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLSNILLLA
                     DRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPTFSLTPEGRLTA
                     RNADISGHISANSGTLNNVVIAENCTIKGTLRAENIIGDVVKTHNVSLPDLRAAGEHR
                     HATERTVTVIDDQSFDRNIVIPPVFYSGIIYKGSDGDGATSICTLTVSVNDRVVFSKT
                     SSIGTRRTSGGYPVQEDTIYPGSSTPFSYFMRMASGGGDVRIKFRIEVIYYGTAPGFM
                     TTGIQITTMKASASGFVES"
BASE COUNT          289 a          337 c          289 g          307 t
ORIGIN      
        1 tcccggtctg cggtaaaaat cgtggtttac aggattaaaa aggtgctatc cggaaccgga
       61 attcacacag tgaacggtgg gtgatccaga taatgagcac tacagtgata caaatcacgg
      121 tggtttttgc gacacagcac ttgctcctta ttacggagag gcgctaacct gccacttgct
      181 taaggtagac ggatcagggc ctcgggttaa cattgaagtt gactcggggc cttttcacat
      241 caggctttca ggtgttccct ccggccatca gccaaaggca cccgcgcata ctgtgcggtt
      301 tttgtctcct tccggcaatc acgatcgtcc gggattaact ttccacaaat cctgaagctg
      361 acgccttcat cgttgtgatc tgtatccctg tggtcataaa acccggtgca gttccataat
      421 agatgacctc aatccggaac ttgatcctga catcacctcc ccctgacgcc atcctcatga
      481 aatagctgaa cggagtggat gacccgggat atatggtgtc ttcctgaacg gggtatcccc
      541 ctgacgtacg cctggtaccg atggatgagg ttttgctgaa caccacccga tcattcacac
      601 tcaccgtcag cgtacagatt gaggtcgcac cgtcaccatc acttcctttg tagataatac
      661 cggaataaaa aaccggcggg ataacaatat tccggtcaaa cgactgatca tcaatgacag
      721 taaccgttcg ttctgtcgcg tgtctgtgct cccctgcagc cctgagatca ggaagactga
      781 cattgtgcgt tttcaccaca tcgccaatga tattttccgc tctcagagta cctttgatgg
      841 tacagttctc cgctatcacg acattattca gcgtgcccga gttcgcactg atatgtccgc
      901 tgatatccgc attacgggcc gtcagcctgc cttccggcgt cagggagaac gtagggggat
      961 tgccggacga ggtgatactc accgcaaaca gccgcttcag gaacacatcg ttcatgaaca
     1021 actgattccc ctgcgccaca aataacggcg tggtgttgcc gtcctccggg ttaatcatcg
     1081 caatacggtc agccagcagc agtatgttgc tcaggggctg gccatcagta tcctcaatcc
     1141 ccgctccaat accggcaaca tagggtatgc cattttttgt tttctgtacc ttcagcatgt
     1201 aaagtgcagc aaggtcatca tt
//
LOCUS       DAZGJT010000289         1219 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZGJT010000289 DAZGJT010000000
VERSION     DAZGJT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.296"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(163..856)
                     /locus_tag="ACHKFK_004984"
                     /pseudo
     CDS             complement(163..856)
                     /locus_tag="ACHKFK_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          363 a          266 c          254 g          336 t
ORIGIN      
        1 atgtatataa aaaaactatt acgcaatcat tagatacccc cagatttagt tgtaaagcaa
       61 gcacactttg catttcgaga caatccaggt aaagaccctt acctcccatt ttgtgatttt
      121 tatacttatg atgataagtt gaacgttggg tagtgcttcc aatcagtaga acatgtgctt
      181 ttcaatgaag gaaccgatga ctttttcgtg gattgcaacg gaacgcgaga agcagattgt
      241 tttacttacc agccgcttga tgcgggtgcg gagggtcagg ttgttacgtt caatacgttg
      301 cgtgaagatt ttgccggtca ggtatttctc cttcggcact tctctggcgt agctgcccca
      361 gtcatcgcgg ccaccatgcc gatacagaac ggagtcaaca gggctaacaa ttcccgacag
      421 gcctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt agtgttctac
      481 gcataccaga gccagtgttg ttgcgccttg ttgccaacga agctccacag ctcatcaagt
      541 tcacagataa gggcaacatc agcgtgagct gactgacgca gatgttatcc gctggggcga
      601 gagcttttta atgtccgtat gacggtattg atgctgattt tcagtgttct ggcgatatca
      661 cggcccctgt gccattatgc gccatttcaa cgatcagctc cttaaagccg ggttttctgg
      721 cttcataaga gtaagtgagc tggaaaacac gtttacatga acgacaacgg aaacgctcat
      781 gctgggaaca actacgacca tgtcgataaa ctttatcgga attacagcgc ggacaatgaa
      841 cagtaacggt agccatggaa aaccttaaat accagtagat taccagaaac ttctatcaat
      901 tgaagacatt accgtttttg attacagcaa atgttttatt aattacccca ccatcgacta
      961 aatccgttag aacgtcgaat gatgccattc gtaaatacat taaaaagact gttgtaatca
     1021 acgaagatgg aaaacagtgt gttaaaacta atctgaactt cagaaaaaca gatgatggta
     1081 tggtgatata tatatccgct ctgaaccgcc acgcaattta aaataaaaat caacaaatat
     1141 cgtcgatatc catatatacc atttttctgt agttgtatta gcgtcgcata ttttatgact
     1201 ttaagtcata agtcaaaac
//
LOCUS       DAZGJT010000290         1218 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZGJT010000290 DAZGJT010000000
VERSION     DAZGJT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.297"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..>1218
                     /locus_tag="ACHKFK_004985"
     CDS             71..>1218
                     /locus_tag="ACHKFK_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO9055102.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSGGEFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGSTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFAS"
BASE COUNT          289 a          262 c          312 g          355 t
ORIGIN      
        1 tttgttgtcg tgataatgac aatcaataaa ggaattctgt atgagtcata cagaaagaac
       61 aggatattaa atgaataaaa aatcgttatg gaagctaatt ctgatattag cgatcccatg
      121 tattattggc tttatgccag ctccggcagg attaagcgaa ctggcgtggg tgctttttgg
      181 tatttacctg gcggccattg tggggctggt tatcaagcct ttcccggaac ctgtcgtact
      241 gttaattgcc gtcgccgcat cgatggtagt ggtgggtaac ttatcaggtg gggaatttaa
      301 aaccaccgcc gtattaagcg gttactcttc cggtaccacc tggctggtgt tttctgcgtt
      361 tactttaagc gccgcgtttg taaccacagg cttaggtaaa cgtattgcct atctgctgat
      421 tggtaaaatt ggtagcacta ccctgggtct gggttacgtt acggtatttc tcgatctggt
      481 actggctccg gcaacaccgt ctaacaccgc gcgtgcgggc ggcatcgtgt taccgatcat
      541 caacagcgtg gcagtggctt tgggatcaga accggaaaaa agtccgcgtc gtgttggaca
      601 ttacctgatg atgtccattt acatggtcac taaaaccacc agctatatgt tctttaccgc
      661 aatggcgggg aacattctgg cgctgaaaat gatcaacgac attctgcacc tgcaaattag
      721 ctggggtgga tgggcgctag ccgccggatt gcctggcatc attatgctgc tggtcacccc
      781 gctggtgatt tacaccatgt atccaccaga aattaagaag gtggataaca aaaccatcgc
      841 taaagcgggc cttgccgaac tgggaccgat gaaaatccgc gaaaaaatgc tgctcggtgt
      901 ctttgtgctg gcgctgctgg gctggatttt cagtaagtct ctgggggttg atgaatccac
      961 cgtggcaatc gttgttatgg caactatgct gctgctgggt atcgttacct gggaagacgt
     1021 ggttaaaaat aaaggcggct ggaatacctt aatctggtac ggcggtatta tcggcttaag
     1081 ctccttatta tcgaaagtta aattcttcga atggttagct gaagtcttta aaaataacct
     1141 ggcatttgat ggtcacggta acgttgcttt cttcgttatt attttcctca gcatcatcgt
     1201 gcgttatttc ttcgcttc
//
LOCUS       DAZGJT010000291         1196 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZGJT010000291 DAZGJT010000000
VERSION     DAZGJT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1196
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.298"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..606
                     /locus_tag="ACHKFK_004986"
     CDS             <1..606
                     /locus_tag="ACHKFK_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFO9055103.1"
                     /translation="TGMATIDEIKQSVSIFINNKVPVDNITILHCNTEYPTPFEDVNL
                     NAINDLKKHFPKNNIGFSDHSSGFYAAIAAVPYGITFIEKHFTLDKSMSGPDHLASIE
                     PDELKHLCIGVRCVEKSLGSNSKVVTASERKNKIVARKSIIAKTEIKKGEVFSEKNIT
                     TKRPGNGISPMEWYNLLGKIAEQDFIPDELIIHSEFKNQGE"
     gene            606..>1196
                     /locus_tag="ACHKFK_004987"
     CDS             606..>1196
                     /locus_tag="ACHKFK_004987"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HFO9055104.1"
                     /translation="MRTKIIAIIPARSGSKGLRNKNALMLIDKPLLAYTIEAALQSEM
                     FEKVIVTTDSEQYGAIAESYGADFLLRPEELATDKASSFEFIKHALSIYTDYESFALL
                     QPTSPFRDSTHIIEAVKLYQTLEKYQCVVSVTRSNKPSQIIRPLDDYSTLSFFDLDYS
                     KYNRNSIVEYHPNGAIFIANKQHYLHTKHFFGRYSLA"
BASE COUNT          424 a          181 c          216 g          375 t
ORIGIN      
        1 acaggaatgg ctactattga tgagataaaa cagtctgttt ctatttttat aaataataaa
       61 gttccggttg ataatattac aatattacat tgcaatactg aatatccaac gccctttgag
      121 gatgtaaacc ttaatgctat taatgatttg aaaaaacact tccctaagaa taacataggc
      181 ttctctgatc attctagtgg gttttatgca gctattgcgg cggtgcctta tggaataact
      241 tttattgaaa aacatttcac tttagataaa tctatgtctg gcccagatca tttggcctca
      301 atagaacctg atgaactgaa acatctttgt attggggtca ggtgtgttga aaaatcttta
      361 ggttcaaata gtaaagtggt tacagcttca gaaaggaaga ataaaatcgt agcaagaaag
      421 tctattatag ctaaaacaga gataaaaaaa ggtgaggttt tttcagaaaa aaatataaca
      481 acaaaaagac ctggtaatgg tatcagtccg atggagtggt ataatttatt gggtaaaatt
      541 gcagagcaag actttattcc agatgaatta ataattcata gcgaattcaa aaatcagggg
      601 gaataatgag aacaaaaatt attgcgataa ttccagcccg tagtggatct aaagggttga
      661 gaaataaaaa tgctttgatg ctgatagata aacctcttct tgcttataca attgaagctg
      721 ccttgcagtc agaaatgttt gagaaagtaa ttgtgacaac tgactccgaa cagtatggag
      781 caatagcaga gtcatatggt gctgattttt tgctgagacc ggaagaacta gcaactgata
      841 aagcatcatc atttgaattt ataaaacatg cgttaagtat atatactgat tatgagagct
      901 ttgctttatt acaaccaact tcacccttta gagattcgac ccatattatt gaggctgtaa
      961 agttatatca aactttagaa aaataccaat gtgttgtttc tgttactaga agcaataagc
     1021 catcacaaat aattagacca ttagatgatt actctacact gtcttttttt gaccttgatt
     1081 atagtaaata taatcgaaac tcaatagtag aatatcatcc gaatggagct atatttatag
     1141 ctaataagca gcattatctt catacaaagc atttttttgg tcgctattca ctagct
//
LOCUS       DAZGJT010000292         1187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZGJT010000292 DAZGJT010000000
VERSION     DAZGJT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.299"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1187
                     /locus_tag="ACHKFK_004988"
     CDS             <1..>1187
                     /locus_tag="ACHKFK_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO9055105.1"
                     /translation="EGLPLPETYDRPDPRIKQLARRSMVTPGGAACKYNDIIPADHCL
                     HDVQDMSNLNHPHADLSKGQYGCVGHALHVAKKLLPFMPANAGILLVPCGRGDSGFTA
                     GAEGAFNEASGATAGSSLWGADKPLYHDLVSRTRAALKKNPKNVLLSVIWMQGEKDVS
                     SGRHAEHNALFLAMVNQFRTELADVAEQCTGGTTASVPWICGDTTYYWKERYAAPYEA
                     VYGGYKGKAAQNIHFVPLMTDEHGVNVPTNEPSEDPDIIPAGYYGAASRTASNWTSAD
                     RKTHFSSWARRGLVSDRLAGAILQYAGRTLSFLTGQSAPQSGGTTPVSPGTPDVEKPQ
                     DGGVAGTGHDEAVSSTRTVAEYDANSGNGVWTEQQWGAAGGKGTVTDDGGRKALRLEK
                     QPG"
BASE COUNT          273 a          276 c          392 g          246 t
ORIGIN      
        1 gaagggctgc cgctgccgga gacatatgac cgtccggacc cgcgtattaa acagctggcg
       61 cgtcgcagta tggtgacgcc gggtggtgcc gcctgtaaat ataacgacat cattccggca
      121 gaccactgtc tgcatgatgt gcaggatatg agtaacctga atcatcctca tgcagacctg
      181 agtaagggac agtacggttg tgtggggcat gctctgcatg ttgccaaaaa gctgctgccg
      241 tttatgcctg cgaatgcggg tatccttctt gttccatgtg gccgtggcga ttcgggattt
      301 actgcggggg ccgagggggc gtttaacgaa gcgtcgggag cgaccgcagg ctcgtccctg
      361 tggggggcgg ataaaccgct gtatcatgac ctggtcagca gaacccgtgc ggccctgaag
      421 aaaaatccga agaacgtgct gttatcagtg atctggatgc agggagaaaa agatgtcagt
      481 tcggggagac atgcagaaca caatgcgctt ttccttgcca tggtaaatca gttccgtacg
      541 gaactggcag atgtggcaga gcagtgtact ggcgggacaa cggccagcgt cccgtggatt
      601 tgcggtgata ccacgtacta ctggaaggaa aggtatgcag cgccgtatga ggcggtttac
      661 ggtgggtata aaggcaaagc tgcgcagaat attcactttg tgccgttgat gacggatgag
      721 catggtgtga atgtgccgac aaacgagccg tcagaagatc cggacattat cccggcggga
      781 tactatggtg ctgcgtcacg cactgccagt aactggacgt cagccgatcg taaaacgcac
      841 ttcagttcat gggcgcgaag aggccttgtt tcagaccgcc tggcaggtgc cattttgcag
      901 tacgccgggc ggacattatc gttccttacc gggcagagcg caccgcagtc gggtggtaca
      961 actcctgtca gtccggggac accggatgtt gagaaaccgc aggatggcgg tgttgctggt
     1021 actggtcatg atgaggccgt gagcagtacc aggacggtgg ctgaatatga tgcgaacagt
     1081 ggaaatggtg tatggacaga gcagcagtgg ggggctgccg gtggcaaagg cactgtgact
     1141 gatgacggtg gacggaaagc gctgcgactg gaaaaacagc ccggtaa
//
LOCUS       DAZGJT010000293         1159 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZGJT010000293 DAZGJT010000000
VERSION     DAZGJT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.300"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..569
                     /locus_tag="ACHKFK_004989"
     CDS             <1..569
                     /locus_tag="ACHKFK_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9055106.1"
                     /translation="QSHLTRELPAILEDGENGLSPFVRTSIYRQSKHIRELEEQVKQV
                     EEALASWYRTQEACQRMAKIPGGGMLTATYVVAAVGNARQFSTAKQFASWLGLTPKEH
                     SSGGKQQLGGISKRGDGYFRYLLVHGARALTAWVNRNGAVEENSWLQGLLERKHYNVA
                     VVAMAAKTARIMWSMLSHNTEYQPRQLA"
     gene            complement(1071..1154)
                     /gene="yohP"
                     /locus_tag="ACHKFK_004990"
     CDS             complement(1071..1154)
                     /gene="yohP"
                     /locus_tag="ACHKFK_004990"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO9055107.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT          322 a          280 c          318 g          239 t
ORIGIN      
        1 gacaaagcca tttaacccgc gagctgccag ccattctgga ggatggcgaa aatggtttat
       61 ctccctttgt cagaaccagc atctacagac agtctaaaca tatccgggaa cttgaagaac
      121 aagttaaaca ggtagaagaa gctctggcct cctggtatag aacgcaggaa gcctgccaga
      181 gaatggccaa aatcccgggg ggtggcatgt taacggccac ttatgtggta gcagcagtgg
      241 gtaatgcccg acaattcagt accgcaaagc agttcgcttc atggctgggg ctgacaccaa
      301 aggaacattc cagcggcggg aaacagcaac tgggagggat cagcaaacgt ggagatggat
      361 atttccgata cctgctggtt cacggcgcac gcgcacttac cgcctgggtc aaccgaaacg
      421 gcgcggttga ggagaattcc tggcttcagg gactccttga gcggaagcac tacaatgtgg
      481 ctgttgtcgc catggcggca aaaacagcga ggatcatgtg gtcaatgttg tcacacaata
      541 ctgaatatca acctcggcag ctcgcctgac acaaggtcag tcaatctgac gaggtgctga
      601 aaaaaagtta acgtcctggt aggtgcaggt gacttcagaa gatggcaaaa caggtaaggc
      661 cgcaggcagg atacaccgag aggtaacaag ggcaagttag cccgtagagg atgattggta
      721 cctgcctggc ggatttcatc atggttcggg ataaaaatcc catcagaaac cggatatatg
      781 gctgcaccta cccgttttat tacataactg aagaaagcat tgcattccgg gagcgtccat
      841 atatgttacc tcgcgcccga cccggtagcc gtaagggata agccgagtgc accgcgcagc
      901 ggcgattttg ttcgccagag cccggggggg cagggggcgg cggcgactgg tcgccccctg
      961 cgcgctcctt gcgccagtga ttatatgaag caatgcacat gacaggagcg caacaggatg
     1021 agaggctgaa ctattctcgt tattttcgga cctgatgaca ttaattaatt ttaaaatatc
     1081 atcttaaata cgccagtcac caccagtagc ccaatcagga aaataatcaa tacagcccag
     1141 agtataattt tcattggcg
//
LOCUS       DAZGJT010000294         1158 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZGJT010000294 DAZGJT010000000
VERSION     DAZGJT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.301"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1158
                     /locus_tag="ACHKFK_004991"
     CDS             <1..>1158
                     /locus_tag="ACHKFK_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD5 domain-containing protein"
                     /protein_id="HFO9055108.1"
                     /translation="PKPDYAKLANEGADADTLAMIALYRSDIPAKTKLNAVKWVESVK
                     SIRTSVAGMLEGKVTAGRLAEWMEGRMPSRYADTWQLLRTLPPSQMDKASAYRVVSGV
                     YQAAGGKRYDPSQKLYSLRNKDNKGSNLFFSESRDELLTKAKAWFAEQEEKSRAKGDE
                     KTAPSPDDKISFDVYRNTRSGDIFIAYGKNKMRVRGGFKSASDARKYIDSHRDELVRH
                     VKEMREISREEQRNATNRDRTGPERRKGDVSPEQFSDAFGFRGVQFGNYVEGPRRQAD
                     LNRAYDSLHDLADVLNVPTKALSLNGRLGLAFGARGKGKAAAHYEPGEVAINLTKGNG
                     PGALAHEWFHSLDNYFGRYDVSTDGKITSGGDYMTEAQRAGRVFKDGRYVDA"
BASE COUNT          294 a          251 c          359 g          254 t
ORIGIN      
        1 ccgaaaccgg actatgcaaa actggcgaac gaaggggccg acgctgacac actggcaatg
       61 attgcgctgt accgcagtga tattcccgcg aaaacgaaac tcaatgcggt gaagtgggtg
      121 gagagtgtaa agagcatccg cacaagcgtg gcgggaatgc tggaaggaaa agtgactgcc
      181 ggacggcttg ctgagtggat ggagggaaga atgccttccc gttatgcgga tacctggcaa
      241 ctgttacgca cgctgccacc ttcacagatg gacaaggctt ctgcttatcg ggtggtatcc
      301 ggtgtgtatc aggcggccgg agggaagcgt tacgatccgt cacagaaact ttattcactg
      361 cgcaataagg acaataaggg gagtaacctc tttttctcgg aaagcaggga tgaattactg
      421 acaaaggcga aagcctggtt tgcagagcag gaggaaaagt cacgggcgaa aggtgatgaa
      481 aaaacagcac cgtcaccaga tgacaaaatc agctttgacg tttaccggaa tacccgtagt
      541 ggcgatattt ttatcgctta cggcaaaaac aaaatgcggg tgagaggtgg ttttaagtca
      601 gccagtgatg cgcgtaagta cattgattca catcgtgatg agcttgttcg tcatgtgaag
      661 gagatgcggg agatttcgcg tgaggagcag cgtaacgcca ctaaccgcga ccgcaccgga
      721 ccagaacgcc gcaaagggga tgtttcgccc gagcagttca gtgatgcgtt tggtttccgt
      781 ggtgtgcagt ttggtaatta tgtggaaggt ccgcgtcgtc aggctgattt gaaccgggct
      841 tatgactcgc tgcatgacct ggctgacgtg ctgaacgttc cgacaaaagc gctttccctg
      901 aatggtcgtc ttgggcttgc ttttggtgcc cgtggtaagg gtaaggcggc ggcacactat
      961 gagccgggtg aggtggcaat caacctgaca aaaggtaacg gaccgggggc gctggcccac
     1021 gaatggttcc attcactgga taattatttt ggtcgttatg atgtttccac tgacgggaag
     1081 atcacgtcag gtggcgacta tatgacggaa gcacaacgtg ccgggcgcgt atttaaagac
     1141 ggcagatatg ttgatgcg
//
LOCUS       DAZGJT010000295         1147 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZGJT010000295 DAZGJT010000000
VERSION     DAZGJT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.302"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..891
                     /locus_tag="ACHKFK_004992"
     CDS             <1..891
                     /locus_tag="ACHKFK_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001539076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9055109.1"
                     /translation="YGDPVDPGWSGFYWIRFVNAAGVKGPWNAVKGTPAQTQISVQAI
                     IDQIKEEAAKSPVVEELRKEIKNAQGQAVKDAAIETTEVVGNLREETLKTIGGIDTRI
                     TGMNKSTSEELNKVNERITKLDKEGGEAFLAMWSKKAGVDGITAGIGIVAGKDSEGRP
                     VSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYDAVIETLVSRKVVADEV
                     KAGVSITSPVIRSAVIQNGNFQVDSQGNLNIGGLFSVTSQGQLTIRYSNQNVGLVIRN
                     DKIEVYDQNGRLAVRIGRLS"
     gene            906..>1147
                     /locus_tag="ACHKFK_004993"
     CDS             906..>1147
                     /locus_tag="ACHKFK_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055110.1"
                     /translation="MAEYGFAIYNRNNVNVTGVLTPIFFLDRFTAESGSKTYTNKPEG
                     KTLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNL"
BASE COUNT          327 a          216 c          332 g          272 t
ORIGIN      
        1 tacggtgatc cggttgatcc gggctggtcg ggattctact ggatacgctt tgttaatgca
       61 gcgggagtga aagggccatg gaacgcggta aaaggcactc ccgcacaaac ccagataagc
      121 gttcaggcca ttattgacca gatcaaggag gaggctgcaa agtcaccggt ggtggaagag
      181 ctgcgtaagg aaataaagaa tgctcagggg caggcagtaa aggacgcggc aatcgagacg
      241 acggaagttg tgggtaatct cagggaagaa acactgaaaa ctatcggtgg tattgatacc
      301 cgtattacgg ggatgaataa gtcaaccagt gaagagctta ataaggtaaa tgagcgcatc
      361 actaaactgg ataaagaagg cggtgaggcc ttccttgcga tgtggtcaaa aaaagcggga
      421 gttgatggca tcactgcggg gatcgggatt gtcgccggaa aagacagtga aggcaggcct
      481 gtaagtcagg ttgcaatttc tgcgtcacag ctgtttgtct ttgacccgaa taatccggat
      541 aacaccgcct atccgtttgc ggtctctggt ggaaaagttg tcatccccaa agcgatgatt
      601 tatgacgcgg tgattgaaac actggtgtcg cggaaggttg tggcggatga ggtaaaagcc
      661 ggggtaagta tcacttcgcc agttatccgg agtgccgtta ttcagaacgg aaactttcag
      721 gttgattctc agggtaacct gaatattggt ggccttttta gtgttacgtc gcaggggcaa
      781 ctgacaattc gttactctaa tcagaatgtg gggctggtga tccgcaatga taaaattgaa
      841 gtttatgacc agaacggacg cctggctgtt cgcataggca ggttaagctg agccggaggc
      901 tgacattggc agaatatggt tttgcaatat acaacagaaa taatgttaat gttacgggcg
      961 tgcttactcc catatttttc ctggacaggt ttacggcgga gtcgggatca aaaacgtata
     1021 cgaataaacc agaagggaaa acattacagg cagtctgttc attatttccg tggaataatg
     1081 tatttaagga ccggaaagta ccgaagataa ccattaatgg caatacggtg acatggtcga
     1141 atcttga
//
LOCUS       DAZGJT010000296         1144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZGJT010000296 DAZGJT010000000
VERSION     DAZGJT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.303"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..272
                     /locus_tag="ACHKFK_004994"
                     /pseudo
     CDS             1..272
                     /locus_tag="ACHKFK_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            800..1072
                     /locus_tag="ACHKFK_004995"
     CDS             800..1072
                     /locus_tag="ACHKFK_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin biosynthesis transcription regulatory
                     family protein"
                     /protein_id="HFO9055111.1"
                     /translation="MTPSPYPRNNFNTELSQSCMNGEHFSLLIEISPIRSKKNIMALK
                     EYLVDGYSKQESCERNNVSISYFCLCLKKLNYTHSIVSALKYFYRE"
BASE COUNT          354 a          229 c          230 g          331 t
ORIGIN      
        1 gagggaagtc ttactgctgt ggccgcagga gaacaggtcg ttcctgcctc tgaacttgct
       61 gctgccatga agcagattaa agaactccag cgcctgctcg gcaaaaaaac gatggaaaat
      121 gaactcctta aagaagccgt tgaatatggg cgtgcaaaaa agtggatagc gcacgcgccc
      181 ttattgcccg gggatgggga gtaagcttag tcagccgttg tctccgggtg tcgcgtgcgc
      241 agttgcacgt cattctcaga cgaaccgatg actggaaata taggggcaaa tccacatcct
      301 tacttttccg taaggatttt agcgggtcaa aacagcgttc catgccggac tgatgatcta
      361 catacttaga gtcacatacc aaagtaaaac tatggatgta tgcaagcctg catcgtcagg
      421 aggttaaatt tcagacctgc cagttaccgc gtaaatggct tgatatatga ctaatttatt
      481 tgcacaggat ttttacgaca acgctctgaa ccagcagttg acgggatata tagttgagct
      541 cattaccgat aaagcttttc tgtatctctg gcggtggtca tagaccagta atcgttattg
      601 gttgttctat attatcccgt atgacggtac aacgttttta acaactggcg ctgtcacaac
      661 ataagccacc ccaaaatatt atatgagtaa caatagtcta tataggagcg taaattacct
      721 actatcaaat acttactcca atagcatttc tgtctattct atttcaattc tttaatagaa
      781 aaataaggtt agataatata tgactccctc cccctatcca agaaataact ttaatacaga
      841 gctaagtcaa tcatgtatga atggagaaca cttttcactt ttaattgaaa tatcaccaat
      901 cagaagtaaa aaaaacatca tggctcttaa ggagtatttg gttgatggtt attcaaagca
      961 agaaagttgt gaaaggaaca atgtttccat tagttatttt tgcctatgcc taaaaaaact
     1021 aaactataca cacagtattg ttagtgctct taaatatttc taccgtgagt aaactgtaac
     1081 tgaagtcttg attcttacgc ttagacactg ctaagccgtt atggcatagc tgataattta
     1141 tatt
//
LOCUS       DAZGJT010000297         1144 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZGJT010000297 DAZGJT010000000
VERSION     DAZGJT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.304"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            202..630
                     /gene="tolA"
                     /locus_tag="ACHKFK_004996"
     CDS             202..630
                     /gene="tolA"
                     /locus_tag="ACHKFK_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO9055112.1"
                     /translation="MYCRAVPVLVLILASLTTGCTKDVSTNNYDASLYHSDKLIKSQN
                     LSSSERTPDASEEIRQYAIQVRKAIEEQLKDASKYSGKECSLRMYMAPNGLLLQVKRE
                     SGDPDLCREAMNAVKNADIPSPPSSGVYKAFRNGVLDFKL"
BASE COUNT          315 a          238 c          271 g          320 t
ORIGIN      
        1 tcagacttcc ttgtttattc tcgccggatg cttcgtagat atttgcaggc atctgtatgc
       61 aggggtatta ttgcggcata tatcatccgg aaaaataaaa actacatgct gatccggaga
      121 tgatagttac aagtcctccc ggatcccgca tatttactat attttataga taattgttct
      181 tgttaatatg gagatggata aatgtattgc cgtgctgtac cagttcttgt tttgatatta
      241 gccagtctga caactggctg cacaaaagat gtatcgacaa ataattatga tgcttctctg
      301 tatcactcag ataaattaat aaagtcgcaa aatttgagct catcggaacg tacccctgat
      361 gccagtgaag aaatcaggca gtatgccatt caggtcagga aagccataga agaacagtta
      421 aaggatgcga gtaagtattc aggaaaagag tgttcactga gaatgtatat ggccccgaat
      481 ggtcttctgc tacaggttaa aagagaaagc ggtgatcccg atttatgtcg tgaagcgatg
      541 aatgcagtaa agaatgctga tataccatca ccaccgtctt caggagtata taaagcattt
      601 agaaatggcg tgctggattt taaactctga cagggggaga agattttctc ctgtcctgat
      661 gattttgggg cgctggaaag atattgtgct gtgaagtgtt aaattcctca caattcagta
      721 agttgacagt tgcctgtcag actgagcatt tgttaaaaaa atttcgcatg gtgaatcccc
      781 ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa gcgagaatat
      841 gcgggtttag tgtcaccggg ctgaactcac cgggaggcac ccggcaccat gcaatggcac
      901 atagcgccac tctccagccc ctctccggag gggctttctt gtgggcaaaa aaaaagcccg
      961 agtgggttcg ggcaacagca tgagatatac atttttataa tcgaatggat tttaaccaga
     1021 attcataagg ctgcgcaact gcgcggcctt tttcgtattg cgggctgtag tcttcctcct
     1081 gccattgtcc tgtaacttcc ggacttcagc ccgcccctca tctgactcac aacattatcc
     1141 cgac
//
LOCUS       DAZGJT010000298         1133 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZGJT010000298 DAZGJT010000000
VERSION     DAZGJT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.305"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            172..>1133
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKFK_004997"
     CDS             172..>1133
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKFK_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO9055113.1"
                     /translation="MSSLNIKQGSDTHFPEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     VFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTP"
BASE COUNT          344 a          224 c          255 g          310 t
ORIGIN      
        1 gtttatcatt tgtttaattt ataaatttgg tgggtatttc tctgaaatag agtgaatgtt
       61 gcattcagtg ggagtaattt acaaacttca gcttcggata aatctcacca actggtagag
      121 tgggggccgt ggagatttac tcttattgga ctcattttca ggttataccc catgtcatca
      181 ctgaatatta aacagggaag tgacactcat tttcccgagt atcctctggc gtcgcccagt
      241 aataatgaaa ttgatttact taatctaatc gaagttttat ggcgggcaaa aaaaacggtc
      301 atggcggtca tttttgcgtt tgcctgcgca ggattgctga tctctttcat cctgccgcaa
      361 aaatggacca gcgcggcggt tgtcacgcct ccagaacctg ttcagtggca agagctggag
      421 aaaacattca ctaagcttcg tgtgctggat ctggatatca aaattgatcg tacagaagta
      481 tttaacctgt ttatcaagaa atttcagtct gttagcttac tagaagagta cctgcgctca
      541 tcaccttatg tgatggatca attaaaagaa gcgaaaatcg acgaactgga tttgcatcgc
      601 gctatcgtcg cattgagcga aaaaatgaaa gcggttgatg acaatgccag taagaaaaaa
      661 gatgaaccgt cactgtatac ctcctggacg ctaagtttta ccgcgccaac cagtgaagag
      721 gcgcagaccg ttttgagcgg gtatatcgat tatatctctg cgttggtggt gaaagagtcg
      781 atagaaaacg tccgtaataa actggagatc aaaacccagt ttgaaaaaga aaaactggct
      841 caggatcgca ttaaaacgaa aaatcaactt gatgcaaaca ttcagcgcct caattattca
      901 ctcgacattg ccaacgcggc aggaattaaa aagcccgtat acagtaatgg tcaggccgtt
      961 aaagatgacc ccgatttctc tatttctctc ggtgcagacg gtattgaacg caaactggaa
     1021 attgaaaaag cggttactga cgttgcggaa ttgaacggtg aattacgtaa tcgccagtat
     1081 cttgtcgagc agttaacaaa agcaaatatc aacgatgtga attttacgcc gtt
//
LOCUS       DAZGJT010000299         1120 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZGJT010000299 DAZGJT010000000
VERSION     DAZGJT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.306"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1072
                     /locus_tag="ACHKFK_004998"
     CDS             <1..1072
                     /locus_tag="ACHKFK_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO9055114.1"
                     /translation="GLDNDNNNRFDKSSATLKNQGIEARDEVKYLQHYIDELRDKVDL
                     TVALVHEGVPARQSSIGNTDVRRALDKDIQTASKVKGLDILITGHAHVGTPEPIKVGN
                     TLILSTDSGGIDIGKLILDVNPTARTHKVKSFELKTVYADEWIPDPTTQKVINGWNKK
                     LADLVRQPVGESSIALTRAYGESSQLGNLFTDAMLVAAPTAQIALINSGSLRADINAG
                     TITFGDITSTFPFKNELTEMDLSGKDLRNLLEHGASLTNGILQMSKGAEMRYTLQKPV
                     GQRIVSFKINGEEIVDTNIYHVATTTFLALGGDGFLAFKEGKNVQVRAGNNMSNVVID
                     YFKKGHKITPAQVNEMRVEVSK"
BASE COUNT          341 a          244 c          256 g          279 t
ORIGIN      
        1 gggactcgat aatgacaata ataaccgatt tgataaatct tccgcgaccc tcaagaatca
       61 ggggattgaa gcacgtgatg aagtgaagta tttgcagcat tatatcgacg agttacgtga
      121 taaggttgac ctcaccgtgg cgctggttca tgaaggcgta ccggcgcgtc agtccagcat
      181 tggcaataca gatgttagac gtgcgctgga taaagatatt caaaccgcaa gcaaggtgaa
      241 aggattggat attctgatta ccgggcatgc ccacgtcgga acgccagaac caatcaaagt
      301 cggcaacacc ttaatccttt cgactgacag tggcggtatc gatattggta aattaatcct
      361 tgatgtcaac cctacagctc ggactcataa agtgaagagc tttgagctga aaacggttta
      421 tgccgatgaa tggatacccg atccaaccac acaaaaagtt atcaatggct ggaataaaaa
      481 actggcggat cttgtacgcc aaccagtcgg tgaatcatcg attgcattaa ctcgtgccta
      541 cggtgagtca tcgcaacttg gcaacctgtt taccgatgca atgcttgtcg ctgcaccgac
      601 tgcacaaatt gcgttgataa actccggcag tttacgtgct gatataaatg caggcacgat
      661 tacctttggt gacattacca gtacattccc cttcaagaat gaacttactg aaatggatct
      721 cagcggtaag gatctgcgta acctactgga acacggggca tcgcttacca atggcatcct
      781 gcaaatgtca aaaggcgcag aaatgcgcta taccctgcag aaaccagtag gtcagcgcat
      841 tgtatctttt aaaattaatg gtgaagagat cgttgatacg aatatttacc atgttgcgac
      901 tacgactttc ctagcacttg ggggcgatgg ttttctggca ttcaaagaag ggaaaaatgt
      961 ccaggtccgc gccggaaata acatgtccaa tgtcgtaatc gattatttta agaaaggcca
     1021 caagattact cctgcacagg taaatgaaat gcgggtagaa gtaagcaaat aacaaaaact
     1081 tataatacca ttcattcact ggatgacatt atttctctct
//
LOCUS       DAZGJT010000300         1091 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZGJT010000300 DAZGJT010000000
VERSION     DAZGJT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.307"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..878)
                     /gene="tdcA"
                     /locus_tag="ACHKFK_004999"
     CDS             complement(<1..878)
                     /gene="tdcA"
                     /locus_tag="ACHKFK_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO9055115.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVE"
BASE COUNT          345 a          247 c          201 g          298 t
ORIGIN      
        1 aattccacca aaaccgatgc tgctttttta atacgataat ttttcgacca taccgcggca
       61 tattgtgcca caggtaatgt ttcttcaacc ggaatagtaa taaattgatt agaaccaaaa
      121 ggtgatgtca tatcacaagg aattacagtt aagaaatcag cattgagaac aagattataa
      181 attgtcacga ctgagtcggt tttaacgatg ttttcaatac tgatgccatt tctttgtaac
      241 gtagtaagta gttcgctgta gtaccccata ttggtttgtg gcaacaccca ctgttcgttc
      301 ttcaacgact ccagcgtggt ggtgccggtg catgttcggg acttactggc taccagcaca
      361 aactcggact cgaacagcgg ctcaacatgt aaatcctgaa gcttcatttc tgcgcttaac
      421 gtaccaatcg caaaatccag tcgaccgtcg cggattgccg gtaagaacga agacagttgc
      481 gcttcataca tagaaacctg cgctttcggg aacacctctt tgaacttgtt gatcatccct
      541 gacataaaag taaaaccaat caatgaagga aaaccaaatg agacttccac caccgcctca
      601 gaagacatac cgcttatctc attaaccata tttttcattt cacgggtaat ggattcggaa
      661 cgggagagta acaattgacc ggcaggtgtt aatgttacac cggtattttt ccgcaccact
      721 aactccacac caaaataatc ctcaatatcg ttaatgattt tactgacggc cggttgagtt
      781 aaccctaact cttttgcagc cgagccgata gaaccacttc taataacttc ctgaaagact
      841 accaggtgct gcgttttcgg aagaagaata gtgctcataa cgacctacgt taattacctc
      901 attgacggca tgaagtgtat caaaatgaaa tgaacaggat atgtgcgacc actcacaaat
      961 taactttcaa tactttccat ggcatcgtta ccccaaacaa atacctttaa atatcaacaa
     1021 gttaaaatat gaaaatcagc ataagccctg atttttgtca aaaaaaatga cggggataac
     1081 catattttat g
//
LOCUS       DAZGJT010000301         1075 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZGJT010000301 DAZGJT010000000
VERSION     DAZGJT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.308"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..558
                     /locus_tag="ACHKFK_005000"
                     /pseudo
     CDS             1..558
                     /locus_tag="ACHKFK_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008826.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(610..978)
                     /locus_tag="ACHKFK_005001"
     CDS             complement(610..978)
                     /locus_tag="ACHKFK_005001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055116.1"
                     /translation="MKKIIFGMSALIMSFSSIAGGGGSWSPSISPEHCVGVSYNPFSV
                     FWTGMKQCQEAIDHGYAQGVGFSGVVSYGNGNESSNMRAFSGVVHPGERLTSNQLGVP
                     ETIGNAKINGTGKIAAFWAQ"
BASE COUNT          307 a          269 c          204 g          295 t
ORIGIN      
        1 agactcatac cccgtattcg caacctcggc agtcgcaggt tgtacgttat ttccccctca
       61 gaggaatata aaccacttga tgccctgatc gccggaaagg ccaaaatgga agtaatagaa
      121 cagcactggg atgaagttct gcggcttaag gcctcgatcg gcgctggtct ggtaccacca
      181 tcggtaatac tgaaaaagct ggcagcatct ccgagacaaa acaggctcaa tctggccctg
      241 cgtgaaatgg gacgtattga acgctcaatt tttatctgtg actggttgct tgacactcgg
      301 ttacgtcgca gatcacacac aatcctcaac aaaggcgaaa gccgccatgc acttgcccgc
      361 gctatttttc ttcaccaact gggcgaattc cggaacaggg ctgccgagac aatggctcac
      421 agggcatcgg gcctcaacct tgttgttaat gcaataattc tgtggaacac ggtctatctc
      481 agtcgggctg ttgattacat tcgcagccag gggatcatta taccggaaga gctcttgtcc
      541 agtgtatcac cactacccta agattcaata ctgacaagag agcagactgc aatgcagcct
      601 gctgttaaat tattgtgccc agaaggcagc aatttttcct gtaccattaa tttttgcatt
      661 tccaatagtt tctggtacac ctaattgatt tgaagtcaat ctttcacctg gatgcactac
      721 tccactgaat gctctcatat tacttgattc attaccgtta ccatatgaaa ccacaccgct
      781 aaaacctaca ccttgtgcat aaccgtgatc aatagcctct tggcattgtt tcatgccagt
      841 ccaaaaaaca gaaaaaggat tataggatac accaacgcaa tgctccggac taatacttgg
      901 agaccatgac cctcctcccc cagcaatact tgaaaaactc atgattaatg ccgacattcc
      961 aaatataatc tttttcataa atagctcctg tttgttttga aattagtttc aaattacaca
     1021 atatttattt cttttttgat ctcctcctta ttcacaggta taaacatata aaaat
//
LOCUS       DAZGJT010000302         1044 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZGJT010000302 DAZGJT010000000
VERSION     DAZGJT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.309"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..430
                     /locus_tag="ACHKFK_005002"
     CDS             5..430
                     /locus_tag="ACHKFK_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003026046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO9055117.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTFSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            427..777
                     /gene="tnpB"
                     /locus_tag="ACHKFK_005003"
     CDS             427..777
                     /gene="tnpB"
                     /locus_tag="ACHKFK_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO9055118.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            808..>1044
                     /locus_tag="ACHKFK_005004"
     CDS             808..>1044
                     /locus_tag="ACHKFK_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9055119.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGPRSEK"
BASE COUNT          278 a          257 c          269 g          240 t
ORIGIN      
        1 aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aactattcga atgaattcaa
       61 acttcgtatg gtggaactgg catcacaacc tggagcttgt gttgcacaga ttgcacgtga
      121 aaatggcgtc aatgataatg ttattttcaa atggctcagg ctctggcaga acgaagggcg
      181 tgtttcgcgg cgtcttccgg taacgacctt ttctgacact ggcgttgaat tattacctgt
      241 agaaataacg ccggatgagc agaaagaacc tgtggcggcc attgcgccgt ctttatccac
      301 ttccactcag accagagtca gtgccagttc ctgcaaggtg gaattccgtc acggtaacat
      361 gacgctggaa aatccatcgc cagagctgct cacggtgttg atccgtgaac tgaccgggag
      421 gggaagatga tctcactccc atcaggtacc cgtatctggc tcgttgccgg tgttaccgat
      481 atgcgtaaat ccttcaacgg actgggagaa caggtacaac atgtgctgaa tgataatccc
      541 ttctccggtc acctgtttat cttccgtggc cgacggggtg acaccgtcaa aattctttgg
      601 gctgatgctg atggtctgtg cctgttcacc aaacgcctgg aggaaggcca gtttatctgg
      661 cctgcggtac gtgacggcaa ggtatccatt acccgctcgc aactggcaat gctcctcgat
      721 aagctggact ggcgtcagcc aaaaacatcc agccgtaact cactgacaat gttgtaaaaa
      781 actcctgacc gcattataaa aacggtcatg agtcagaaat acctcattcg catcgcagag
      841 ctggaaaggt tgctctctga gcaggctgaa gccctccgtc agaaagacca gcaactgagt
      901 ctggttgaag agacggaagc cttcctgcgc tctgcactga cacgtgccga agaaaagatc
      961 gaagaagatg aacgggaaat agaacatctg cgggctcaga tagaaaaact gcgccggatg
     1021 ctgttcggtc cccgttctga aaaa
//
LOCUS       DAZGJT010000303         1036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZGJT010000303 DAZGJT010000000
VERSION     DAZGJT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.310"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1036
                     /locus_tag="ACHKFK_005005"
     rRNA            <1..1036
                     /locus_tag="ACHKFK_005005"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          254 a          242 c          326 g          214 t
ORIGIN      
        1 gctaactccg tgccagcagc cgcggtaata cggagggtgc aagcgttaat cggaattact
       61 gggcgtaaag cgcacgcagg cggtttgtta agtcagatgt gaaatccccg ggctcaacct
      121 gggaactgca tctgatactg gcaagcttga gtctcgtaga ggggggtaga attccaggtg
      181 tagcggtgaa atgcgtagag atctggagga ataccggtgg cgaaggcggc cccctggacg
      241 aagactgacg ctcaggtgcg aaagcgtggg gagcaaacag gattagatac cctggtagtc
      301 cacgccgtaa acgatgtcga cttggaggtt gtgcccttga ggcgtggctt ccggagctaa
      361 cgcgttaagt cgaccgcctg gggagtacgg ccgcaaggtt aaaactcaaa tgaattgacg
      421 ggggcccgca caagcggtgg agcatgtggt ttaattcgat gcaacgcgaa gaaccttacc
      481 tggtcttgac atccacggaa gttttcagag atgagaatgt gccttcggga accgtgagac
      541 aggtgctgca tggctgtcgt cagctcgtgt tgtgaaatgt tgggttaagt cccgcaacga
      601 gcgcaaccct tatcctttgt tgccagcggt ccggccggga actcaaagga gactgccagt
      661 gataaactgg aggaaggtgg ggatgacgtc aagtcatcat ggcccttacg accagggcta
      721 cacacgtgct acaatggcgc atacaaagag aagcgacctc gcgagagcaa gcggacctca
      781 taaagtgcgt cgtagtccgg attggagtct gcaactcgac tccatgaagt cggaatcgct
      841 agtaatcgtg gatcagaatg ccacggtgaa tacgttcccg ggccttgtac acaccgcccg
      901 tcacaccatg ggagtgggtt gcaaaagaag taggtagctt aaccttcggg agggcgctta
      961 ccactttgtg attcatgact ggggtgaagt cgtaacaagg taaccgtagg ggaacctgcg
     1021 gttggatcac ctcctt
//
LOCUS       DAZGJT010000304         1036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZGJT010000304 DAZGJT010000000
VERSION     DAZGJT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.311"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            31..225
                     /gene="bfd"
                     /locus_tag="ACHKFK_005006"
     CDS             31..225
                     /gene="bfd"
                     /locus_tag="ACHKFK_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO9055120.1"
                     /translation="MYVCLCNGISDKKIRQAVRQFSPHSFQQLKKFIPVGNQCGKCVR
                     AAREVMEDELMQLPEFKESA"
     gene            297..773
                     /gene="bfr"
                     /locus_tag="ACHKFK_005007"
     CDS             297..773
                     /gene="bfr"
                     /locus_tag="ACHKFK_005007"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO9055121.1"
                     /translation="MKGDTKVINYLNKLLGNELVAINQYFLHARMFKNWGLKRLNDVE
                     YHESIDEMKHADRYIERILFLEGLPNLQDLGKLNIGEDVEEMLRSDLALELDGAKNLR
                     EAIGYADSVHDYVSRDMMIEILRDEEGHIDWLETELDLIQKMGLQNYLQAQIREEG"
     gene            complement(802..>1036)
                     /gene="gspO"
                     /locus_tag="ACHKFK_005008"
     CDS             complement(802..>1036)
                     /gene="gspO"
                     /locus_tag="ACHKFK_005008"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prepilin peptidase GspO"
                     /protein_id="HFO9055122.1"
                     /translation="CHKEGLGYGDFKLLAAAGAWCGWQTLPMILLIASLGGIGYAIVS
                     QLLQRRTITTIAFGPWLALGSMINLGYLAWISY"
BASE COUNT          290 a          215 c          270 g          261 t
ORIGIN      
        1 tacttttgaa gaaagtatac gggagtctct atgtacgttt gtctttgtaa tggtatcagc
       61 gataaaaaaa ttcgtcaggc tgtgcgccag ttttcccccc actcgttcca gcaattaaaa
      121 aaattcattc cggtcggcaa tcagtgcggt aaatgtgtcc gggccgcgcg tgaagtgatg
      181 gaggatgaat taatgcaact gccggagttt aaggagtccg cataaacgga gtgtgttttt
      241 tgacttactc gtaagccgtt ctacgcttca aagagtggaa gcgaaggagt caaaaaatga
      301 aaggtgatac taaagttata aattatctca acaaactgtt gggaaatgag cttgtcgcaa
      361 tcaatcagta ctttcttcat gcccggatgt ttaaaaactg gggtctcaaa cgtctcaatg
      421 atgtggagta tcatgaatcc attgatgaga tgaaacacgc cgatcgttat attgagcgca
      481 ttctttttct ggaaggtctt ccaaacttac aggacctggg caaactgaac attggtgaag
      541 atgttgagga aatgctgcgt tctgatctgg cacttgagct ggatggcgcg aagaatttgc
      601 gtgaggcaat tggttatgcc gatagcgttc atgattacgt cagccgcgat atgatgatag
      661 aaattttgcg cgatgaagaa ggccatatcg actggctgga aacggaactt gatctgattc
      721 agaagatggg actgcaaaat tatctgcaag cacagattcg cgaagaaggt tgagccgtgt
      781 cgggagggag aaccctcccg gttaataaga gatccaggcc agatatccca ggtttatcat
      841 gctgccgagc gcaagccacg ggccgaatgc gatagtggtt atagtccggc gttgcagaag
      901 ttgtgaaacg atggcgtaac caatgccacc cagcgaggca atcagcagta tcattggcaa
      961 cgtttgccag ccacaccacg cgcctgcagc ggccagtagc ttgaaatcac cgtagcccaa
     1021 tccctctttg tgacaa
//
LOCUS       DAZGJT010000305         1016 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZGJT010000305 DAZGJT010000000
VERSION     DAZGJT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.312"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            226..>1016
                     /locus_tag="ACHKFK_005009"
     CDS             226..>1016
                     /locus_tag="ACHKFK_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011478180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntS/YbdA MFS transporter"
                     /protein_id="HFO9055123.1"
                     /translation="MQRKTLLSACIALALSGQGWAADITEIETTTGEKKNTNVTCPAD
                     PGKLSPEELKRLPSECSSVVEQNLMPWLVTGAATALITTLAIVELNDDDDDHHRNNSP
                     LPPTPPDDDSDDTPVPPTPGGDEIIPDDGPDDTPAPPKPISFNNDVTLDKTAKTLTIR
                     DSVFSYTENADGTISLQDNNGRKATINLWQIDETNNTVALEGMSADGATKWQYNHNGE
                     LVITGDNTTVNNTGKTIVDGKGATGTEIAGNNAVVNQDGELDVSGG"
BASE COUNT          311 a          214 c          225 g          266 t
ORIGIN      
        1 ataaatatct atgtgacgca tttgaattta taatttatat aatatagata atttgtatgt
       61 ttagcaggtt tatttatttc tctgtattat aaatccagat tgtcagaaaa tatgtttgat
      121 taggtaacaa tctactggca atattggacg tcctctatgt tttaaataat caattggtcg
      181 ggtcagggta tccgaccttc tttatattcg ccagaaggat ttattatgca aaggaaaact
      241 ctattgtcgg cctgtattgc attagctctg agtggtcagg gttgggcggc agatattaca
      301 gaaatagaaa ccaccacagg tgaaaagaaa aataccaatg tgacttgtcc agcagatcct
      361 ggaaaactca gtccagaaga gcttaaacgc ttaccctctg aatgctcctc tgtagtcgaa
      421 cagaacctga tgccatggct tgtcacaggt gctgctactg cgttaatcac aaccttagcc
      481 atagtggaac taaacgacga tgatgatgat catcatcgca ataattctcc acttccacca
      541 actccacctg atgatgattc agacgacact ccagttcctc caactcctgg cggagatgag
      601 ataataccgg acgacggtcc ggatgatact cctgcacctc ccaaaccgat ttcgtttaat
      661 aatgacgtta ccctggataa aacagcaaaa acgttaacta ttcgcgattc agttttcagt
      721 tataccgaga atgctgacgg gactatttct ctgcaagata acaatgggcg taaggcgact
      781 attaatcttt ggcagattga tgaaacgaat aacacggttg cccttgaagg gatgagcgca
      841 gatggtgcaa cgaagtggca atataatcac aacggtgagc tcgttattac gggcgacaat
      901 accacagtaa acaacactgg aaaaaccatc gtcgacggca aaggcgccac cggaactgaa
      961 atcgcgggta acaacgcggt ggtgaatcag gacggcgagc tggatgtgag cggcgg
//
LOCUS       DAZGJT010000306         1008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZGJT010000306 DAZGJT010000000
VERSION     DAZGJT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.313"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..371)
                     /locus_tag="ACHKFK_005010"
     CDS             complement(<1..371)
                     /locus_tag="ACHKFK_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO9055124.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNP"
     gene            complement(371..700)
                     /locus_tag="ACHKFK_005011"
     CDS             complement(371..700)
                     /locus_tag="ACHKFK_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9055125.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVMLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(697..>1008)
                     /locus_tag="ACHKFK_005012"
     CDS             complement(697..>1008)
                     /locus_tag="ACHKFK_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001621422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO9055126.1"
                     /translation="VHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMR
                     RAEGINFNQNNHVVIQNDGPNGRAGPQLMKAVYEMARKGAQDELRLQLRDGGMLSGSG
                     G"
BASE COUNT          211 a          372 c          217 g          208 t
ORIGIN      
        1 tccggattgc ccgcaacgaa attcaccgca tccagaaaac gggcatacac ccggcggcgg
       61 accaccgtgg cccccaccag gctctgcaaa tcctccgcca tcccggtgac cagaccgaac
      121 agattggaca ccgtcagcga cggtctggca ctgctgcccc ggccgttcat ctcaaaaccg
      181 ctgccgtcaa tcgggtatgc ctcatactta cgcccctgcc aggtgaccgg ctcccctttt
      241 tcattcagct cattacagaa aaaataccgc tcaccgccct gtaccgtcag gtcgatttcc
      301 cagagtacca cccgcggtga ctgctctgat ttaaccgact cgttcagact ttcttcgtga
      361 atatcctgca tcagttcacc acctgcttaa actccgcgct gaactcaacg cgcaacatcc
      421 cgacccgcgc agaccacccg gcacaggtca cctttatctg ccggtatgca tagggtggct
      481 tccacaaaaa tgccttccag ccaccgtgct ctgccagaaa cgcttccaga tgccgggcct
      541 cctcccgggt cacggaaagc atcacccggt atgttttcag gtcagcattc agccctgccg
      601 ccatacgctg tgagtacccg tcaccaaaac gcacttcacg caccgatggc tgcgagttca
      661 cctccatatc cggcttcact ttccagcgaa aggttttcat ccaccgctcc ctgataacat
      721 accgccatca cgcaactgca gccggagttc atcctgtgcc cccttgcggg ccatctcata
      781 caccgctttc atcagctgcg gccctgcccg cccgttgggg ccgtcgttct gaatcaccac
      841 gtgattgttc tgattaaaat taatgccttc cgcccgccgc atctgcgccg gacttccggc
      901 accgccgaca taaccacctt ccgcatagcc ccgcatcagg cggtacagat tgccgacacc
      961 aatccggctg gtcgcctcct tcgtgaagac aaactccccg cgatgaac
//
LOCUS       DAZGJT010000307         1002 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZGJT010000307 DAZGJT010000000
VERSION     DAZGJT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.314"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..993
                     /locus_tag="ACHKFK_005013"
     CDS             <1..993
                     /locus_tag="ACHKFK_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFO9055127.1"
                     /translation="LAHVVTGKYADHLPLYRQSEIYRRQGVELSRATLGRWTGAVAEL
                     LEPLYDVLRQYVLMPGKVHTDDIPVPVQEPGSGKTRTARLWVYVRDDRNAGSQMPPAV
                     WFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESGRITEAACMAHARRKIHDVH
                     ARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKARAAPLMQSLYDWIQQQMKT
                     LSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIGNNIAENALRGVAVGRKNWMFAGSDS
                     GGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPANRVRDLLPWKVDLSSQ"
BASE COUNT          239 a          266 c          302 g          195 t
ORIGIN      
        1 ctggcccatg ttgtcaccgg gaaatatgca gaccatctgc cgttataccg ccagtcagaa
       61 atataccgtc gtcagggagt ggagctgagc cgtgccacac tggggcgctg gacaggtgct
      121 gttgctgaac tgctggagcc gctgtatgac gtcctgcgcc agtatgtgct gatgcccggt
      181 aaagtccata ccgatgatat ccccgttccg gtccaggagc cgggcagcgg taaaacccgg
      241 acagcccggc tgtgggtcta cgtccgtgat gaccgtaacg ccggttcaca gatgcccccg
      301 gcggtctggt tcgcgtacag tccggaccgg aaaggtatcc atccacaaaa tcacctggcc
      361 ggttacagcg gtgtgcttca ggccgatgct tacggtggtt accgggcgtt atacgaatcc
      421 ggcagaataa cggaagccgc gtgtatggct catgcccgga gaaaaatcca cgatgtgcat
      481 gcaagagcgc ccacctacat caccacggaa gccctgcagc gtatcggtga actgtatgcc
      541 atcgaggcag aggtccgggg ctgttcagca gaacagcgtc tggcggcaag aaaagccaga
      601 gccgcgccac tgatgcagtc actgtatgac tggatacagc aacagatgaa aacactgtcg
      661 cgtcactcag atacggcaaa agcgttcgca tacctgctga aacagtggga tgcactgaac
      721 gtgtactgca gtaatggctg ggtggaaatc ggcaacaaca tcgcagagaa cgccttacgg
      781 ggagtggccg taggccggaa aaactggatg ttcgcgggtt ccgacagcgg tggtgaacat
      841 gcggcggtgt tgtactcgct gatcggcaca tgccgtctga acaatgtgga gccagaaaag
      901 tggctgcgtt acgtcattga acatatccag gactggccgg caaaccgggt acgcgatctg
      961 ttgccctgga aagttgatct gagctctcag taaatatcaa ta
//
LOCUS       DAZGJT010000308          987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZGJT010000308 DAZGJT010000000
VERSION     DAZGJT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.315"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..386
                     /locus_tag="ACHKFK_005014"
     CDS             <1..386
                     /locus_tag="ACHKFK_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055128.1"
                     /translation="VLPAIFTHSVIKLSGISDFKIHSYIIKTSEYPEEFFSNAVWGKK
                     NLKPGEYYSVQAVSMFTTNQFILLCPKDIIRFYRESWKFDLLNVDFDTNTRKKLQEEA
                     AYCVPISAISVKRWDMPLQGSKPSN"
     gene            complement(612..787)
                     /locus_tag="ACHKFK_005015"
                     /pseudo
     CDS             complement(612..787)
                     /locus_tag="ACHKFK_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          306 a          199 c          200 g          282 t
ORIGIN      
        1 ccgtattacc agcaattttt acccattcag ttattaagct ctcaggtata agtgacttca
       61 aaatacatag ctacattatt aaaacaagtg aatatccgga ggagtttttt tccaatgcag
      121 tatggggtaa aaaaaattta aaaccggggg aatattactc tgtgcaggct gtttctatgt
      181 ttacgacaaa ccagtttatt ctcctttgtc ccaaggatat tataaggttt tatcgggaaa
      241 gctggaagtt cgacctgctt aatgtagact tcgatacaaa taccaggaag aaactccagg
      301 aagaagcagc atattgcgta ccaatttctg caatatctgt aaaacgatgg gatatgcctc
      361 ttcagggctc gaaaccctca aattagaagg ccgctcgtgc gactgtatta gatagtaatg
      421 cggtatggaa aaatagtgat ggcaagatga acttgacttg gctaatcaaa taccggcgta
      481 gtgattagga aacagggctc ggataacatt attgcgcggt tcattgaacc tactgatgac
      541 gccggatata tagtcgtaaa ccgtttcgaa ccagtactta tatcaccagg agagtccata
      601 tatgaccacc gtttacagta aaaacggtat ctgttttctg atccttccag cgtcaaacta
      661 ccgttgtaat ggtgaggtct ggtgctattg taataattca gaatataatc tctgatttat
      721 ttccgaacct cgcccttgcc tgcgtaacca tccgtcgccc ccatcctgtt ttcagactgc
      781 ggaaaaaggc aagtttttat ccccccacag agattcaatc atatatcgcg tgttttggaa
      841 gtgacagttc ttattcctga gaatactgca tgtgcaacac actcggcatc catcgatacc
      901 acttttatct ctaataggca ggataaaaaa caagactgtg aattaaagta atcggcaaat
      961 gaatgagtta gaagatactg aaaatga
//
LOCUS       DAZGJT010000309          982 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZGJT010000309 DAZGJT010000000
VERSION     DAZGJT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.316"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..719)
                     /locus_tag="ACHKFK_005016"
     CDS             complement(15..719)
                     /locus_tag="ACHKFK_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO9055129.1"
                     /translation="MKFNLSNLSAVLLASGMLMSTAVTAAPGDAAQFGGADTDWSTVD
                     YPRLTDMDDNVDSMGGKIRFTGRVVKATCKVATDSKQIEVVLPVVPSNLFTGIDVEAQ
                     GASNQTDFNINLTECSNTEDQKIEFRFTGTADSANKTLANEVEGSTDADNSGNAGATG
                     VGIRIYSKGTTNNGLINLNTTAAEGSASTAAYTIPGNATTHDFSAAFTAGYAQNGSTV
                     APGVVKSTASFVVLYE"
BASE COUNT          271 a          218 c          200 g          293 t
ORIGIN      
        1 aatcgtattt atatttactc gtacagcacc acaaaacttg ctgttgactt aactacacca
       61 ggtgcaacag tgctaccgtt ttgagcataa cctgcagtaa aggccgcgct gaaatcatgg
      121 gtcgtagcat ttcctggaat tgtataagcg gcggtggagg cgctaccctc tgccgcagta
      181 gtattcaggt taatcagacc attattcgtc gtacctttgg agtaaattcg aatccctaca
      241 ccagtcgccc ccgcattgcc gctgttgtca gcatccgttg atccttctac ttcgttagcg
      301 agcgttttat tagcgctatc tgcagtaccg gtaaaacgga actcaatttt ctgatcctct
      361 gtattgctac attcggtcag attaatattg aaatcggtct ggttgctcgc cccctgtgct
      421 tctacgtcga taccagtgaa aaggttggaa ggcacaaccg gcaggacaac ttcaatctgt
      481 tttgaatcgg ttgcgacctt acaggtagct ttcactacac ggccagtaaa gcggattttc
      541 ccccccattg aatcaacgtt gtcatccata tcagtgagtc tgggataatc aacggtgctc
      601 cagtcagtat ccgccccacc aaattgtgct gcatcgccgg gtgctgcggt taccgcagta
      661 gacatcagca tacctgatgc cagtaatact gcggataaat tagataaatt gaatttcatt
      721 tatacctttc cttttgttaa aaaatccaag caacattggt gtttttacat tctgcgttaa
      781 taagttatat ctcacaaaaa ataagctgac gttggctaat tccagaaggt tctttagaag
      841 agtaattgag tgaataatat tatatatgct gcttagtaat gtacctaaaa taatccaaag
      901 ccgcaataag tgagtttata ctcacatata acatactgat aatgataaaa tttattttta
      961 cgtggggttg taataggggc aa
//
LOCUS       DAZGJT010000310          974 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZGJT010000310 DAZGJT010000000
VERSION     DAZGJT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.317"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>974
                     /locus_tag="ACHKFK_005017"
     CDS             <1..>974
                     /locus_tag="ACHKFK_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO9055130.1"
                     /translation="VVVGVPVPDNIHPMTKDEAGVWSWRTPVLKGNLYEYFFNVDGVR
                     SIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVVHGDLIAITYHSNALQSERQMYVWTP
                     PGYTGIGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETD
                     AKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQ
                     GGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNEPAAINQQLRNFTVVVGD
                     KDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQK"
BASE COUNT          247 a          220 c          266 g          241 t
ORIGIN      
        1 agtagtggtg ggtgttccgg ttccggacaa tattcacccg atgaccaaag acgaagcggg
       61 agtctggtcg tggcgcacgc ctgtcctgaa aggcaatctg tacgagtatt ttttcaatgt
      121 tgatggtgta cgcagcattg atacaggcac ggcaatgact aagcctcagc gtcaggttaa
      181 ctccagtatg attctggtac cgggcagtta tctggataca cgttctgttg tgcatggtga
      241 tttgatcgcc ataacttacc actccaacgc attgcaatcc gaacgtcaga tgtatgtctg
      301 gaccccgcca ggatacaccg gcataggcga gcctttgcca gtgctctatt tctatcacgg
      361 ctttggtgat accggacgtt ccgctattga tcaggggcgt atcccgcaaa tcatggataa
      421 cctgctggct gaagggaaaa ttaaaccgat gctggtggtg atcccggata ccgaaaccga
      481 tgcgaagggc attattcccg aagatttcgt tcctcaggag agacgtaaag tcttttatcc
      541 gttaaatgct aaagcggcag atcgcgaact gatgaacgat attatcccgc tgattagcaa
      601 gcgttttaat gtccgtaaag atgccgatgg tcgcgcactg gcagggcttt cacaaggtgg
      661 gtatcaggca ctggtatcgg gaatgaatca tctggaaagc tttggctggc tggccacatt
      721 cagtggtgtt accacgacga ccgtaccaga tgaaggggtc gcagcccggc tgaacgagcc
      781 ggcagctatt aaccagcaac tacgtaattt tactgtggtt gttggggata aagatgttgt
      841 aaccggcaag gatatcgccg ggctgaaaac tgagcttgag cagaaaaaaa ttaagtttga
      901 ttaccaggaa tatccgggtc tgaaccatga aatggatgtc tggcggcctg cctatgcagc
      961 cttcgtacag aaat
//
LOCUS       DAZGJT010000311          973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZGJT010000311 DAZGJT010000000
VERSION     DAZGJT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.318"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..534)
                     /locus_tag="ACHKFK_005018"
     CDS             complement(<1..534)
                     /locus_tag="ACHKFK_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO9055131.1"
                     /translation="MIYPEITGKSGEHLRLKTLEAVWIQGRLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIE"
     gene            complement(543..908)
                     /locus_tag="ACHKFK_005019"
     CDS             complement(543..908)
                     /locus_tag="ACHKFK_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO9055132.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLIDDEQFDEINIVR
                     GQPVSGGRLGVKIYKIESE"
BASE COUNT          232 a          287 c          188 g          266 t
ORIGIN      
        1 ctctatacca aaagccataa ccattggttt gtacaaaata aattctgcca cctttagcca
       61 atgctcaatg cgtctttcac aggttcttaa actccattcc gggtgtgcat cattcagcag
      121 ttcagccatt ttgcgcttag tcatcccccg ccccacataa cgctgactca ggacattgag
      181 cagcccggga tagcctgcca ggacttcgcc aataaccctg tcgattatta acgcctctga
      241 atcggtacaa tgtgccagcc agcttttttg attgccgttg atcatatccc gcaaaaaagc
      301 ctcaagttca ggtttgtcca gaccagcttt tttcatcctc cggagcgcct cgttaattgc
      361 cgtttttgtc agctttttag aggtcagtaa ttggttgaac atatttcccg tcttaccgcc
      421 gccaatatac gaccaacgcc cccacatccg taatcgccct tggatccaga ctgcttccag
      481 cgtttttagg cgtaaatgct cgccgctttt gcctgtaatt tccggatata tcatatttac
      541 gctcactcac tctcaatttt gtaaatcttc acacccagcc gtccaccaga tactggctga
      601 ccacgtacaa tattgatttc atcaaactgc tcgtcatcaa tgagcactcc cgcatgcgtc
      661 agcgcatcca gcggtgcttt cagaatgttg tccaggtcgc gacgacgctt atccggtggc
      721 tctgcaatca cctttatcgc cagccttccg gacaggctta atttcagccg ctgctggcga
      781 acaataagcg ccactgcccg gcgataacgc tccccggctt ttgatacaaa atatgtgctg
      841 ccacggcgtc gccagtaagt gttcaccgtc ggcgggtaag gcaaaacaaa ttctatgcgt
      901 tcggtcattt atgctttcca cttcagaaca cccgaatttc tcgcgtgcat taaaaaacga
      961 atcagcaaca aca
//
LOCUS       DAZGJT010000312          936 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZGJT010000312 DAZGJT010000000
VERSION     DAZGJT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.319"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..492
                     /locus_tag="ACHKFK_005020"
     CDS             <1..492
                     /locus_tag="ACHKFK_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO9055133.1"
                     /translation="RTVAVEEAKNLLSKGGEIAVRFKIPDGSELVNGQFVFGLYWPVS
                     QWASGAAANSMLASFFLQTDASNLNLMHHKGTSNAQLGTFGAFDHNWHTVVFRFAGNN
                     SERVVPVIDGTEQTAFDLVMWTNDGFTADTLTLTDITGAKATYPVLLDTVTVKVNESR
                     ASS"
     gene            632..826
                     /locus_tag="ACHKFK_005021"
     CDS             632..826
                     /locus_tag="ACHKFK_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFO9055134.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWRRQIDKRAAEKI
                     SASQSAGAKTEAPLIPEQPS"
     gene            852..>936
                     /locus_tag="ACHKFK_005022"
     CDS             852..>936
                     /locus_tag="ACHKFK_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO9055135.1"
                     /translation="MSEIKSLVTAEAVKEVLRSEEVRSALKQ"
BASE COUNT          262 a          204 c          255 g          215 t
ORIGIN      
        1 cgtactgttg cagtggagga ggcaaaaaat cttctcagca aggggggtga aattgccgta
       61 cggttcaaaa ttccggatgg aagcgaactg gtgaacggac agtttgtctt tggtctctac
      121 tggccggtgt cgcagtgggc gtcaggcgcg gcagcaaaca gtatgctggc atcattcttc
      181 cttcagacgg atgcatcaaa tctgaatctt atgcaccaca agggcacgtc gaatgcacaa
      241 ctgggtacat ttggtgcgtt tgaccataac tggcatacgg ttgttttccg ctttgcggga
      301 aataacagcg aaagagtcgt tccggtgatt gatggtacag agcagacggc gtttgacctt
      361 gtgatgtgga caaatgatgg ctttacagca gatacgctga cgctgacaga tatcacgggg
      421 gcaaaagcga cgtatcctgt actgcttgat acggtcacag tcaaagttaa cgaaagccgg
      481 gcatcatcat aaccggcaaa aaaagccgcc agcggcagga acggaagctg gcggaggtaa
      541 tcccaatgga gaatgtaaag aaaagatgct ttcgtatatc ggttttttaa atgaaaacag
      601 ttctcattgt caaccataac ggtaagaaac tatgacattt attcatcagg tgatgctgta
      661 cttctgtacg gcggtctgtg tgctgtatct tctttcgggt gggtacaggg cagtgcgcga
      721 tttctggcgc aggcagattg ataaaagggc cgcagagaaa atcagcgcca gtcagtcagc
      781 cggagcaaaa acagaagccc cactcattcc ggaacaacct tcttaataac ccctttcaac
      841 gagaaaatcc tatgtcagaa ataaaatcgc tggtcactgc tgaggcagtg aaggaagtcc
      901 tgcgctctga agaagtccgg agcgcactga aacagc
//
LOCUS       DAZGJT010000313          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZGJT010000313 DAZGJT010000000
VERSION     DAZGJT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.320"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..323
                     /locus_tag="ACHKFK_005023"
     CDS             <1..323
                     /locus_tag="ACHKFK_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001824829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9055136.1"
                     /translation="MEAANYTFEHNGRKWDYGKSTQTRLEPSVAAVKAGKLPEQFFWT
                     DADNNDVPMDAETLIALSAAAEQAMFTKGLEIHVRQRTMKKEIEALGDAEAILAYKVG
                     MADR"
BASE COUNT          290 a          164 c          246 g          227 t
ORIGIN      
        1 cgatggaagc ggcgaactac acgtttgagc acaatgggcg taaatgggac tacgggaagt
       61 caacgcagac gcgtcttgag ccgtcggtgg cggcagtgaa agcggggaaa ctgccggagc
      121 agtttttctg gacggatgcg gacaataatg acgtaccgat ggacgctgaa acactgattg
      181 ctctgagtgc tgctgcagag caggcgatgt ttaccaaagg tctggaaatt catgtcagac
      241 agcgcacgat gaaaaaagag attgaagcgc tgggtgatgc ggaagctata ctggcgtata
      301 aggtcggcat ggctgacagg tgaaaaaaag aggggcgctc cagccccttg ctacaaagct
      361 atggctaaat aaagaatact caggtcgagg aaataatctg gcaatgataa ttaaaggaga
      421 tgcaacggcc tccttcaccc ctgtcaaaga taaaaaactg gggagtgcgt gctccccgag
      481 agcatgatac ttgtggttga tagttatttt tatccggcga caaagatatt gagatgaggt
      541 cgcggatgca atacagaatt ttcgtattgc atggtaactg ctgattcctg aaactgaaag
      601 tcgtcatgaa actgtaagcg aaaaaacggg gagacggcac gctccccaag gtcatacatg
      661 agatagtggt gtttttatag tcgaaagaat aattgcataa aaagagtgta aattaaacat
      721 tcaggggata cagaattttc gtaggctgag ggattgctga aaataattta gtgcgtcctt
      781 caatggtcat tcaatagtca tcagattgtg ctgatgtaaa cagttaagca caatttgatt
      841 atttgacttt cttctttttg tgactaactt cacaattaaa gatgtaaccg tatacaaatt
      901 gttgatgaat aaagagacag cataagt
//
LOCUS       DAZGJT010000314          918 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZGJT010000314 DAZGJT010000000
VERSION     DAZGJT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.321"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            11..796
                     /locus_tag="ACHKFK_005024"
     CDS             11..796
                     /locus_tag="ACHKFK_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055137.1"
                     /translation="MNLKKVCCICVLFSLLAGCASESPIDEKKKKTQTTQISINKNVP
                     QQLTDKDLYGNETTLAVSEEDIQAALEGDEFRVPLNSPVILVQSGSRAPETIMQEEMR
                     KYYTVATFSGIPDRKKTVTCNKDKNKDESLDIVDAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWTDYKNQKLTDTMSLRYLVRFTLVDVATGEWATWSPVNYE
                     SKVIFPQIGNKNSDSLDVAEQQISQLKQKTYAAVVKDLVNRYQ"
BASE COUNT          301 a          153 c          200 g          264 t
ORIGIN      
        1 gtgtccgatt atgaatttaa aaaaggtttg ttgtatatgt gtattattct cgctgcttgc
       61 aggatgtgcc tctgaatctc ctattgatga aaaaaagaaa aaaactcaaa ccacacaaat
      121 ttctattaat aaaaacgttc cccagcaact aacagacaaa gatttatatg gtaatgaaac
      181 cacactggct gtatccgagg aggatattca agctgcgctt gaaggagatg agtttcgtgt
      241 gcccctcaat tctcctgtaa tacttgttca gtctggcagt cgcgcaccgg aaaccattat
      301 gcaggaggag atgagaaaat attatacagt tgccacattt tctggcattc ctgacaggaa
      361 aaaaactgta acctgtaata aagacaaaaa caaagatgaa agcctggata ttgtagatgc
      421 cgaaaatatg aactggatgc aggcattgcg ttttgttgca gcaaaaggcc atcagaaagc
      481 gattattgtt ttccaggata cgttgcagac aggaaaatat gactctggat tgaaatcaat
      541 agtttggaca gattataaaa atcaaaaatt gactgacacg atgtcactgc gttatttggt
      601 tcgattcacg ttggtggatg tggctacagg tgagtgggct acctggtcgc ctgtgaatta
      661 tgaatcgaaa gtgatttttc cgcagatagg gaataaaaat tctgacagcc ttgatgtggc
      721 agaacagcag atttcacaac ttaagcagaa aacgtatgca gcagtggtta aagacctggt
      781 gaatcgatat cagtagtgag gttttatacc agacgtttta gtggtactta gcaagaaaga
      841 gcatttctct tttctatttt acactatgga acaagtctgc taattaattt tacatatatt
      901 ttttaggagt ggtaaaaa
//
LOCUS       DAZGJT010000315          906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZGJT010000315 DAZGJT010000000
VERSION     DAZGJT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.322"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>906
                     /locus_tag="ACHKFK_005025"
     CDS             <1..>906
                     /locus_tag="ACHKFK_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD1 domain-containing protein"
                     /protein_id="HFO9055138.1"
                     /translation="FKGVMKAINSSDMPRRSALLDEVRSKPYWSTDVEMAARAFERYV
                     QDKARTAGVENDYLVNIRKAPEHNTDNTWAYPTNAELDGGIRDAFDHLFSTLKTRETD
                     KGVAFYSRKGVTRTPEGNLISDVNRSAEAKGSPVPQVEAVARGVMSGIKDSDLKVRVV
                     KSQKEAEALAGESFDGYGRVHAFYRPDKREIVLVADNIPDGRTVREKLRHEIIHHAME
                     HVVTPAEYQTIIKTVLKTRDSDNATIREAWRKVDASYGKESPEVQAGEFLAHMAEKQP
                     NKFVAAWERVVALVKGVLRRTGLLKP"
BASE COUNT          220 a          207 c          286 g          193 t
ORIGIN      
        1 tttaaaggcg tgatgaaagc gatcaacagc agtgacatgc cgcgtcgttc agcgcttctc
       61 gatgaggtgc gctcaaaacc gtactggtca acggatgttg aaatggcggc gcgtgccttt
      121 gagcgttatg ttcaggataa agcgcgtacg gctggcgtgg agaatgatta tctggtcaat
      181 atccgtaagg cacctgagca caacacagat aacacttggg cttatccgac gaatgcggaa
      241 ctggatggcg gtattcgtga tgcattcgat cacctgttca gtacgctgaa aacccgtgag
      301 acggacaagg gcgttgcgtt ttattcccgt aagggcgtga cccgcacacc tgaaggcaat
      361 ctcatttcgg atgttaaccg cagtgcggaa gccaaaggca gcccggtccc gcaggttgaa
      421 gcggttgccc gtggcgtgat gagcggcatt aaggacagtg acctgaaggt ccgtgtggtg
      481 aagtcacaga aagaggctga agcgctggcg ggggaatcat tcgacggtta cggcagggtg
      541 cacgcgttct atcgtccgga taaacgtgaa attgtcctgg tggcggataa catccctgac
      601 gggcggaccg ttcgcgagaa gctgcgtcac gagattatcc accatgcaat ggagcatgta
      661 gtcacgccag cggaatatca gacgattatt aagaccgtgc tgaaaacccg tgacagtgat
      721 aacgccacca tccgtgaagc ctggcgtaag gttgatgcgt cttatggtaa ggaatcaccg
      781 gaagtgcagg cgggtgaatt tctggcacat atggcggaga aacagccgaa taaatttgtg
      841 gcggcgtggg agcgtgttgt tgccctggtc aaaggggtac tgcgtcgcac ggggttactg
      901 aagccg
//
LOCUS       DAZGJT010000316          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZGJT010000316 DAZGJT010000000
VERSION     DAZGJT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.323"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>905
                     /locus_tag="ACHKFK_005026"
     CDS             <1..>905
                     /locus_tag="ACHKFK_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9055139.1"
                     /translation="DPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAG
                     LWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRILSIDAATRTLT
                     LDREVTLPETGTSTVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLP
                     SLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQH
                     LTVEVSAADGQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITADTEHRFSG
                     LPPGEYTLTVRAINSYGQQGEPATTT"
BASE COUNT          192 a          274 c          287 g          152 t
ORIGIN      
        1 gacccgcaga acggctggca gacctccacg gaactggtgg aagacccgga agccatactg
       61 cgctacggac gcaatctgct gaagatggat gcgtttggct gtaccagccg cggtcaggct
      121 caccgtgcag gtctgtgggt gataaagacc gaactgctgg aaacgcagac ggtggacttc
      181 acgctcgggt cacaggggct gcgtcacaca ccgggtgaca ttattgaaat ctgtgataac
      241 gactatgccg ggactatgac cggcggacgt atcctgtcca ttgatgctgc cacccgcacc
      301 ctgacgctgg accgtgaggt taccctgccg gagacaggta catcgacggt gaacctgatt
      361 aacggcagcg gtaagccggt gagtgtggac atcaccgcac accccgcgcc ggaccggata
      421 caggtcagta ccctgcctga tggtgtggag acatacgggg tgtggggact ctccctgccg
      481 tcactgcgcc gtcgcctgtt ccgctgtgtc tccatccggg aaaacacgga cggcaccttt
      541 gccatcacgg cagtgcagca cgtaccggaa aaagaagcca tcgtggataa cggggcccgc
      601 tttgagccgc agtcaggcac cctgaacagc gttatcccac cggcagtgca gcacctgacg
      661 gtggaggtga gcgcagctga cggccagtat ctggcgctgg cgaaatggga cacgccgcgg
      721 gtggtgaagg gcgtgcgctt cagtctgcgc ctgaccagtg gaagcggtga gaacagccgc
      781 ctgctgacca ccgccatcac tgccgatacg gagcaccgtt tcagtggcct gcctcccggg
      841 gaatacaccc tgacggtcag ggcgattaac agctatggcc agcagggcga accggccacc
      901 accac
//
LOCUS       DAZGJT010000317          905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZGJT010000317 DAZGJT010000000
VERSION     DAZGJT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.324"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>905
                     /locus_tag="ACHKFK_005027"
     CDS             <1..>905
                     /locus_tag="ACHKFK_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9055140.1"
                     /translation="DPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAG
                     LWVIKTELLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAATRPLT
                     LDREVTLPGTGASTVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLP
                     SLRRRLFRCVAIRENTDGTFAVTAVQHVPEKEAIVDNGATFEPQSGTLNSVIPPAVQH
                     LTVEVSAADSQYLALAKWDTPRVVKGVRFSLRLTSGSGENSRLLTTAITAGTEHRFSG
                     LPLGEYTLTVRAINSYGQQGEPATTT"
BASE COUNT          186 a          276 c          291 g          152 t
ORIGIN      
        1 gacccgcaga acggctggca gacttccacg gaactggtgg aagacccgga agccatactg
       61 cgctacggac gcaacctgct gaagatggat gcgtttggct gtaccagccg cggtcaggct
      121 caccgtgcag gtctgtgggt gataaagacc gaactgctgg aaacgcagac ggtggatttc
      181 acactcgggt ctcaggggct gcggcacaca cccggtgaca tcattgaaat ctgtgataac
      241 gactatgccg ggaccctgac cggcggacgt gtcctgtcca tcgatgctgc cacccgcccc
      301 ctgacgctgg accgtgaagt gacacttccg gggacaggtg catcgacggt gaacctgatt
      361 aacggcagcg gtaagccggt gagtgtggac atcaccgcac accccgcgcc ggaccggata
      421 caggtcagta ccctgcctga tggtgtggag acatacgggg tgtggggact ctccctgccg
      481 tcactgcgcc gtcgcctgtt ccgctgtgtg gccatccggg aaaacacgga cggcaccttt
      541 gccgttacgg cggtgcagca cgtaccggaa aaagaggcca tcgtggataa cggggccacc
      601 tttgagccgc agtcaggcac cctgaacagc gtcatccctc cggcagtgca gcacctcacg
      661 gtggaggtga gcgcggctga cagtcagtat ctggcgctgg cgaaatggga cacgccgagg
      721 gtggtgaagg gcgtgcgctt cagtctgcgc ctgaccagtg gaagcggtga gaacagccgc
      781 ctgctgacca ccgccatcac tgccggtacg gagcaccgtt tcagtggcct gcctctcggg
      841 gaatacaccc tgacggtcag ggcgattaac agctatggcc agcagggcga acccgccacc
      901 accac
//
LOCUS       DAZGJT010000318          858 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZGJT010000318 DAZGJT010000000
VERSION     DAZGJT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.325"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..286
                     /locus_tag="ACHKFK_005028"
     CDS             <1..286
                     /locus_tag="ACHKFK_005028"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055141.1"
                     /translation="FDALFPQIALDNTNLTIIQSNYSLLNKGYGFERLQKLGGLLSKF
                     SVGISSNLVINIQNNGGTIVINKDWKIDESLYLRSKVEPFIILEKNIFIA"
BASE COUNT          314 a          144 c          142 g          258 t
ORIGIN      
        1 ttttgatgct cttttcccac aaatagcact cgacaatact aatctgacta taattcaatc
       61 taactatagt ttacttaata aaggatatgg ctttgagcga ttacaaaagc taggtggatt
      121 attatcaaaa ttttctgttg gtataagtag taatttagtt ataaatattc aaaacaatgg
      181 cggaacaatc gttataaaca aagactggaa aatagatgaa agtctttatt tgagatccaa
      241 agtagagcct tttattattc ttgaaaaaaa tatttttatt gcataaaaac atcagcaaaa
      301 tataaattga cgtcaaccag tagtgctata ttgttatgtg gagaaaggag tcaattaaat
      361 gtgttttcaa catggattta ccaagatgaa gagatcatac cggcaaatat tagacatgca
      421 attaaggaga aactgtcaac cagaggtgca ccgtgacaaa atcggtatca tccagtaaga
      481 cgcaactgac caatatacac cagatgcttc gcaatgaata ttgcaaagct caaatgatga
      541 gcctgacgct gataaccatt atccaaaatg gcattagcat acttcacgaa acatccaaag
      601 tgaaattatc tttatcgaag gtcactgtgt tgcatgttgt ccgtaataat tgatctttac
      661 ggagtatgtc gttatcaatt acatctacat aagccattcc tgactgtgaa atatctacta
      721 atatacaata atatagcccg gaatctactt tcttccaaga agaattttat aaatatagtc
      781 tcgcccagag gaaaacatat aatgctggca gatatccaaa tgtgaatatc gctattatgc
      841 aggagactta catatcta
//
LOCUS       DAZGJT010000319          839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZGJT010000319 DAZGJT010000000
VERSION     DAZGJT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.326"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          261 a          154 c          156 g          268 t
ORIGIN      
        1 attatatgga gctaaaaata ctttaaattg acacgtttta actccagatg cttttgatat
       61 ccaggataaa aatagtgttg tagttattcc tcctgcgtca tatttttcag tgaataatac
      121 gtgcgttcac tgttatattt tgacgactat ttatccgtga ctccagctct ccattcagtg
      181 ttccgaacag ataaaatcga gtattttcat acggtatacc aagttaaaat attcaataaa
      241 agcgttctgc gttgacttgt tcgactggat taactctagt cattactgaa tgaaccagtg
      301 ccagaaagat aaattcagga ttgatataca tttaaacatg gcgggatatc taccgtttgc
      361 cgctatttta tctagtatac gatccactca tggcggtggt agcttaaatc tacagcatct
      421 caggaataaa gttattaacg acattcaagg tgcgaaaacg gctactacat acgaggcaga
      481 cggacgataa tgataaactg ttctgctcag gttcatactc aatcagcatg acgtatactg
      541 agtcaaaacg tcgtgctcag atgatgagtg gccagctcat gcttaaaggc tacttttaaa
      601 gttttccgat aacatctttc ttcccccggt aatcagacat gggactgaag acatatttaa
      661 tgattcatcc ggggttaaga tatttaatca cggtaataat ctaaagctta gtaataatgt
      721 cgttacgcat ggagatctct ttcattgtct aaatgtgaat ggcaagaata tgtggactac
      781 ttacgacatc acaagcttaa ttgtaatgga taataggaat attgcctact ttttatatc
//
LOCUS       DAZGJT010000320          824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZGJT010000320 DAZGJT010000000
VERSION     DAZGJT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.327"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..509)
                     /locus_tag="ACHKFK_005029"
     CDS             complement(<1..509)
                     /locus_tag="ACHKFK_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO9055142.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPSGQRAHKPFIFTVALNKAVPLLYNALASGEMLPKVELHWWRTS
                     VEGKQEHYFTTRLTDATIVDMNLHMPHCQDPAQREFTQLLAVSLAYRKVEWEHIKSGT
                     SGADDWRAPL"
BASE COUNT          224 a          207 c          182 g          211 t
ORIGIN      
        1 agcggtgcac gccagtcatc ggcaccagaa gtaccggatt tgatgtgctc ccactcaacc
       61 ttacggtagg ccagtgatac tgcgagcaat tgcgtaaact cacgctgtgc agggtcctgg
      121 cagtgcggca tatgcaggtt catgtccaca atcgtcgcgt cagtaagacg tgtggtgaag
      181 tagtgttcct gcttgccttc aacagaggta cgccaccagt gcagctccac tttcggcagc
      241 atttcaccgg aggccagcgc gttgtacagc agcggaacgg ctttgttcag cgccacggtg
      301 aaaatgaacg gtttgtgagc acgctgcccg gaaggctgac cagactgcgg atcggttgga
      361 acagtaacgt tatgcagaaa ctcctgcacc agcatttcat cttcgtgtcc ctgcacgtag
      421 atattgccga cagaatctgc ggtgaaagca ccggcagtga tgttcccctg ggtctgacct
      481 gtaattgaaa tgtaacatgg ggttggcatt tatgaactcc ttgtgaaatt atcaactgct
      541 gttttacgaa caacccgtcg ttaatccccg acaggttaga gtgtgatgcg gctcacatca
      601 ggaaccacac ccttttcttt attcgagtta caggcacacg taattagcac ctaccgaaaa
      661 actcttttat ggtgtttaaa tatattttta gtcaatttca gaacacgatc acataagcta
      721 aaaatatatc aacatcaaca gtagaattat ccataacgta tccttacccc ataaaaacct
      781 acaatttctt caaaaatcaa tcattacaac acttacttca caat
//
LOCUS       DAZGJT010000321          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZGJT010000321 DAZGJT010000000
VERSION     DAZGJT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.328"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>813)
                     /locus_tag="ACHKFK_005030"
     CDS             complement(<1..>813)
                     /locus_tag="ACHKFK_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO9055143.1"
                     /translation="AESLYITPSAVSQSLQRLRAQFNDPLFIRSGKGIAPTTTGLNLH
                     HHLEKNLRGLEQTINIVNKSELKKNFIIYGPQLISCTNNNILIRCLRQDTSIDIECHD
                     ILLSAENAEELLVQRKTDLAITLQPVISRSVICMPLHTIRNSLICSNKHPRITDTSNY
                     EQIIAEEFTLLISKSAGIDEIQMDIDERFMNRKVSFRSSSLMTIINSISVTDLLGIVP
                     IELYDLHRDFLKLKEIKLEQPLPAVKLYISYNKASLNNLVFSRFIDRLNDSF"
BASE COUNT          250 a          132 c          176 g          255 t
ORIGIN      
        1 agaagctatc atttaaacga tctatgaatc gggagaagac aaggttgttc aacgaagcct
       61 tgttatagga aatataaagt ttaaccgcag gaagaggttg ttccagtttt atttctttta
      121 acttcaaaaa atcacggtgt aaatcgtaca gttcgatggg cacaatgcct aataagtcag
      181 taacagaaat actattaatt atagtcatca aagaagaact gcgaaatgaa actttacggt
      241 tcataaaacg ttcatctatg tccatttgaa tttcgtcaat ccctgctgat ttagatatca
      301 atagggtgaa ttcttcggcg ataatttgtt catagttact ggtatcagta atacgaggat
      361 gtttgttgct acatataagg gaattgcgaa tagtgtgtaa cggcatgcag atgacagagc
      421 gactaattac tggttgcagc gtaattgcca ggtcagtttt gcgttgaacc aacagttctt
      481 cggcattttc agctgaaagc agtatgtcat ggcattctat atcgattgaa gtatcttgtc
      541 taagacagcg gattagtatg ttgttattag tacaagaaat aagctgagga ccgtagataa
      601 taaaattttt ctttagttca gacttattaa cgatattgat cgtttgttca aggcctctga
      661 gatttttctc aagatggtga tgcaggttta accctgttgt agtcggcgcg atacctttcc
      721 ctgagcgaat gaataacggg tcattaaatt gcgcacgtaa acgttgtaac gactgactaa
      781 cagcagaggg cgttatatat aatgattctg ccg
//
LOCUS       DAZGJT010000322          813 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZGJT010000322 DAZGJT010000000
VERSION     DAZGJT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.329"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>813
                     /locus_tag="ACHKFK_005031"
     CDS             <1..>813
                     /locus_tag="ACHKFK_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055144.1"
                     /translation="IDTLPDGKNAVTDTVGKGLKVTGKAVSGGAKASDEWLTGKMSPG
                     AQRALNTPMTEGYDDSAVWVAKGVNLIGALVPDMVAGGVTKKLGEVTLRKVLTAGLEK
                     KYIAAGMQPERATALAAEAVDKKMPDLFQAGLITHSSASAQGQSAMAAAGSVLNADYS
                     ELAKSPKFQQTFLSIDADPQHAQLTDRQKMDLAKERVADEVRAQLATDPELLAVNAMA
                     AKLGDAQLFNLAMRGTAKTVKSGIVRNATEQGTINAAQGGYSRYQENTALRE"
BASE COUNT          208 a          185 c          259 g          161 t
ORIGIN      
        1 tcatcgacac tctgcctgat ggaaaaaatg cggttactga tactgtcggt aaaggtctga
       61 aggtcacagg caaggcagtc agtggcggcg ctaaagcctc ggatgaatgg ctgactggca
      121 agatgtcgcc gggggcgcag cgggcgctga atacaccgat gactgaagga tatgatgatt
      181 ctgcggtctg ggtggcgaag ggcgtaaacc tgattggtgc gcttgtaccg gatatggttg
      241 caggcggtgt gactaaaaag ctgggtgaag tcactctgcg aaaagtgctg accgccgggc
      301 tggagaaaaa atacattgcg gcagggatgc agccggaaag agctaccgca ctggcagcag
      361 aagctgtcga taaaaaaatg ccggatttat tccaggcagg cctgatcacc cattcctcag
      421 ccagcgcaca ggggcagagt gcaatggcag cagcaggctc tgttcttaac gcggattact
      481 ctgagctggc gaagtccccg aaatttcagc agacgttttt gtcaattgat gcagatccgc
      541 agcacgcaca gcttactgat cgccagaaaa tggatctggc aaaagagcgt gttgccgatg
      601 aggtgcgcgc gcagctggca accgatcctg aattgctggc tgtgaatgcc atggcggcaa
      661 aactgggcga tgctcagttg tttaatctgg cgatgcgggg cacggcgaag accgttaaaa
      721 gcggcattgt cagaaatgcc acggaacagg ggacgattaa tgcggcgcag ggtggctatt
      781 cacgctatca ggaaaacacg gcattgcgtg aga
//
LOCUS       DAZGJT010000323          797 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZGJT010000323 DAZGJT010000000
VERSION     DAZGJT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.330"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..797
                     /locus_tag="ACHKFK_005032"
                     /pseudo
     CDS             80..797
                     /locus_tag="ACHKFK_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          238 a          195 c          200 g          164 t
ORIGIN      
        1 tattatcata tcttttaaaa acggctcact aagaaccttg aggtggcaca ctaaatttag
       61 ctacctgaac agaggtgata tgctcacctc agaacaacac aggtgccata atgaaaaaag
      121 aaatttcagc gcagagttta aacgcgaatc cgctcaattg gtcgttgacc agaaatacac
      181 cgtggcagat gcagccagcg ctatggatgt cggcctttcc acaatgacgc gatgggtgaa
      241 acaattacgt gatgagcggc agggaaaaac accaaaaacc tcccccatta ccccggaaca
      301 aattgaaatc cgtgagctca ggaaaaagct acaacgtatt gaaatggaaa atgaaatatt
      361 aaaaaggcta ccgcgctctt gatgtcagac tccctgaaca gttttcgata atcgggaaac
      421 tcagggcgcg ttatcctgtg gccactctct gccatgtgtt cggggtccat cgcagcagct
      481 aaaaatactg gaaaaaccgt cctgaaaaac cagacggcag acgggctgta ttacgtagcc
      541 aggtacttga actgcatggc atcagccacg gctctgccgg agcaagaagc atcgccacaa
      601 tggcaaccca gagaggctac cagatggggc actggcttgc tggcagactc atgaaagagc
      661 tggggctggt cagttgccag cagccgactc accggtataa gcgtggcggt catgagcacg
      721 ttgctatccc gaatcatctt gagcgacagt tcgccgtaac ggaaccaaat caggtgtggt
      781 gcggtgatgt gacctat
//
LOCUS       DAZGJT010000324          779 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZGJT010000324 DAZGJT010000000
VERSION     DAZGJT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.331"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..480
                     /locus_tag="ACHKFK_005033"
     CDS             <1..480
                     /locus_tag="ACHKFK_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9055145.1"
                     /translation="GQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAA
                     YLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEYPGYTLYDLSERLRLRGWQVPAF
                     TLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          194 a          191 c          169 g          225 t
ORIGIN      
        1 ggtcagattg gtactttcgc catcaacttc tcccgcccgg cgggtcaggt gattgcacag
       61 tattatgaat tcctgcgcct cggtcgtgaa ggctatacca aagtacagaa cgcttcctac
      121 caggttgctg cttatctggc ggatgaaatc gccaaactgg ggccgtatga gttcatctgt
      181 accggtcgcc cggacgaagg catcccggcg gtttgcttca aactgaaaga tggtgaatat
      241 ccaggataca ccctgtacga cctctctgaa cgtctgcgtc tgcgcggctg gcaggttccg
      301 gctttcactc tcggcggtga agccaccgac atcgtggtga tgcgcattat gtgccgtcgc
      361 ggcttcgaaa tggactttgc tgaactgttg ctggaagact acaaagcctc cctgaaatat
      421 ctcagcgacc acccgaaact gcagggtatt gcccagcaga acagctttaa acacacctga
      481 taacatcacg ttgtaaaaac cgaatgccca acctttaaaa aacaggtggg gtattcggct
      541 atttattaat tgcaataata tattacctgt ctattcatcg tgttgataag atatatgtta
      601 tatgaatgtt atggagcata gcgatactgc ttaatattac gatgatgaat tatttgcttg
      661 ctattaattc ctctgcgttc ttaagtcccc tttttatata ataaatccag tcatccagcc
      721 cgagtatcat cgctcattat catcctgctt tcccgcttcg tttaaattta tttatcaat
//
LOCUS       DAZGJT010000325          778 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZGJT010000325 DAZGJT010000000
VERSION     DAZGJT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.332"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..191)
                     /locus_tag="ACHKFK_005034"
     CDS             complement(<1..191)
                     /locus_tag="ACHKFK_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055146.1"
                     /translation="MAYSDEQRPEAQFGNQNRNSLNIQQPGETDSYDAFFSDPNRWKD
                     NSTSFSLGDVLPTMGKGVA"
     gene            complement(260..>778)
                     /locus_tag="ACHKFK_005035"
     CDS             complement(260..>778)
                     /locus_tag="ACHKFK_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055147.1"
                     /translation="TEYGSAHPDDQTVLAVPVDKAVELVRDRSKFAEISKNFGYFTPK
                     QQGLSLAKLQEGASSVAAEAIKNGGNAQAAVDEYYAATGSPQHQQKIQQQKIQQGITS
                     WAGDDPDKQAFAREVASRQPEMLEPQNQKLLENGYANFLRIQKARGEQARDDSAASAS
                     EFIRGLKQNYAQ"
BASE COUNT          174 a          218 c          181 g          205 t
ORIGIN      
        1 tgggcgacac ctttccccat agttggcaat acatcgccca ggctgaacga cgtgctgttg
       61 tccttccagc gattgggatc ggagaaaaac gcatcatagc tgtcagtctc gcccggctgc
      121 tgaatgttca ggctgttgcg gttctggttg ccaaattgcg cttcagggcg ctgttcatcg
      181 gaataagcca tacagactcc ataaaaaaac ccggcacaat gaccgggtat caggaaatag
      241 tattaacggg aatggtgaat tactgcgcgt aattctgttt cagaccacgg ataaactcag
      301 atgcagaggc cgcactatca tctctggcct gttcccccct ggccttttga atacggagaa
      361 aattcgcata cccgttctcc agcaatttct gattctgagg ctccagcatt tctggctgac
      421 gggaagcaac ttctctggcg aatgcctgct tatccggatc atcacccgcc caactggtaa
      481 tcccttgttg aattttttgc tgctgaattt tctgctgatg ttgcggtgaa ccagttgcag
      541 cgtaatactc atccaccgcc gcctgagcat tcccgccgtt cttgatcgct tccgccgcta
      601 cactgctggc tccttcctga agtttcgcca gagaaagtcc ctgctgcttc ggcgtgaaat
      661 agccgaaatt tttcgaaatt tccgcgaatt tactgcgatc cctgacaagc tcaacagcct
      721 tatcaacagg caccgccagc acggtttgat catcaggatg tgcactgccg tattccgt
//
LOCUS       DAZGJT010000326          776 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZGJT010000326 DAZGJT010000000
VERSION     DAZGJT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.333"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..612)
                     /locus_tag="ACHKFK_005036"
     CDS             complement(<1..612)
                     /locus_tag="ACHKFK_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055148.1"
                     /translation="MYGLSITKPDGSLWISPGFTPQCLINKGTIPATEKAFFKTSIPS
                     GKSCFFFIRTEKKADVMYTHEQIDGYHALRLHQIVRGTNPGVTTVYAFANMVTQPSEY
                     GIAMYNPSGEMIYHGEMMLLDAKLIPVDIKFEKDLGYPCAIMPALVGYYNWKRTPYDR
                     PIYTTSTGATGNKIYSCEHYSGGATWDIRKPYVDKVLVINTSMY"
     gene            complement(618..>776)
                     /locus_tag="ACHKFK_005037"
     CDS             complement(618..>776)
                     /locus_tag="ACHKFK_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055149.1"
                     /translation="PDGKTLQAVCSLFPWNNVFKDRKVPKITINGNTVTWSNLEQGMG
                     SYIYTFWG"
BASE COUNT          225 a          184 c          140 g          227 t
ORIGIN      
        1 atacattgaa gtattaataa caagaacttt gtctacataa ggctttcgaa tatcccatgt
       61 tgcaccacct gaataatgct cacaggaata tattttattt cctgtagcac cagtggatgt
      121 ggtgtaaatc ggtcggtcat aaggtgttcg tttccagtta taatatccaa ccagtgcagg
      181 cataattgcg catggatatc caaggtcctt ctcaaacttg atatcaactg gtattaactt
      241 cgcgtcaagc agcatcattt cgccatggta aatcatctca cccgacgggt tatacatggc
      301 gataccatac tcagaaggtt gagtaaccat attcgcaaaa gcataaacag tcgtaacacc
      361 gggattcgtt cccctgacaa tttgatgaag ccttaaggca tgatatccat caatttgttc
      421 atgcgtgtac ataacatcgg cctttttctc tgttctgata aagaaaaaac aactttttcc
      481 tgacgggatt gacgttttaa aaaaagcctt ctcagtcgcc ggaatagttc ccttattgat
      541 cagacactga ggcgtaaacc ccgggcttat ccacaagctg ccatctggtt tcgtaatgct
      601 taaaccatac atacaactca cccccagaat gtataaatat atgaccccat cccctgttca
      661 agattcgacc acgtcaccgt attgccatta atggttatct tcggtacttt ccggtcctta
      721 aatacattat tccacggaaa taatgaacag actgcctgca atgttttccc gtcggg
//
LOCUS       DAZGJT010000327          771 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZGJT010000327 DAZGJT010000000
VERSION     DAZGJT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.334"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>771
                     /locus_tag="ACHKFK_005038"
     rRNA            <1..>771
                     /locus_tag="ACHKFK_005038"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          217 a          168 c          236 g          150 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcctcttaa tggggtgact
      181 gcgtaccttt tgtataatgg gtcagcgact tatattctgt agcaaggtta accgaatagg
      241 ggagccgaag ggaaaccgag tcttaactgg gcgttaagtt gcagggtata gacccgaaac
      301 ccggtgatct agccatgggc aggttgaagg ttgggtaaca ctaactggag gaccgaaccg
      361 actaatgttg aaaaattagc ggatgacttg tggctggggg tgaaaggcca atcaaaccgg
      421 gagatagctg gttctccccg aaagctattt aggtagcgcc tcgtgaattc atctccgggg
      481 gtagagcact gtttcggcaa gggggtcatc ccgacttacc aacccgatgc aaactgcgaa
      541 taccggagaa tgttatcacg ggagacacac ggcgggtgct aacgtccgtc gtgaagaggg
      601 aaacaaccca gaccgccagc taaggtccca aagtcatggt taagtgggaa acgatgtggg
      661 aaggcccaga cagccaggat gttggcttag aagcagccat catttaaaga aagcgtaata
      721 gctcactggt cgagtcggcc tgcgcggaag atgtaacggg gctaaaccat g
//
LOCUS       DAZGJT010000328          769 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZGJT010000328 DAZGJT010000000
VERSION     DAZGJT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..769
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.335"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>769
                     /locus_tag="ACHKFK_005039"
     CDS             <1..>769
                     /locus_tag="ACHKFK_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9055150.1"
                     /translation="LLDQALFIPHLKCGIVAQDKQAASEIFRTKIAVPFDHLPDWLRA
                     SFTIVERRSGASGGYILFGHGSSIQVATSFRSGTVQRLHISEHGKICAKYPAKAKELR
                     TGTLNAVSDECIIFDESTAEGVGGDFYEMSNRAQEITASGLALTPQDYKFHFYAWWQD
                     LKYSARVPESGLKLSREKTAYFSAVEKAMNITLTDEQKHWYICKETEQREEMKQEFPS
                     TPQEAFLTSGRRVFSAESTLQAESFCSPPLIVYDIEPV"
BASE COUNT          202 a          184 c          201 g          182 t
ORIGIN      
        1 atcttctcga ccaggcatta ttcataccgc atctcaaatg cgggattgtc gctcaggata
       61 aacaggctgc cagtgaaatt ttccgcacca aaattgctgt accgtttgat catcttcctg
      121 actggctgag agcctcattc accatcgttg aacgtcgtag cggagccagc ggtggctata
      181 tcctgtttgg tcacggctcg agtatccagg tggcaacctc attccgttca ggtacggtgc
      241 agcgcctgca tatctcagag cacggcaaaa tttgcgcaaa atatccggct aaggcgaaag
      301 aactgcgaac cggtacactt aatgccgtct ctgatgagtg cattattttt gatgaatcca
      361 ctgctgaagg tgtgggtggc gatttttacg agatgagtaa ccgggcacag gaaatcactg
      421 catcagggct ggcgctgacg ccacaggatt ataaattcca tttttacgcc tggtggcagg
      481 atctcaaata cagcgccaga gtgccggaaa gcggacttaa gctgtcacgg gaaaaaacag
      541 cgtatttttc tgcggttgaa aaagcaatga acatcacgct taccgatgag cagaaacact
      601 ggtacatctg caaggaaact gaacagcgtg aggaaatgaa gcaggaattt ccctcaacgc
      661 cacaggaggc gtttctgacg tcaggacgac gggtgttcag tgcggaaagc acgctgcagg
      721 cagagtcatt ctgttcgcca ccgctgattg tttatgacat tgaaccggt
//
LOCUS       DAZGJT010000329          760 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZGJT010000329 DAZGJT010000000
VERSION     DAZGJT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..760
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.336"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>760
                     /locus_tag="ACHKFK_005040"
     rRNA            <1..>760
                     /locus_tag="ACHKFK_005040"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          202 a          173 c          236 g          149 t
ORIGIN      
        1 ggttcctgtc caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg
       61 cgtagtcgat gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg
      121 gagaaggcta tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc
      181 aggcaaatcc ggaaaaccaa ggctgaggcg tgatgacgag gcactacggt gctgaagcga
      241 caaatgccct gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa
      301 accgacacag gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa
      361 ctaggcaaaa tggtgccgta acttcgggag aaggcacgct gatatgtagg tgaagcgact
      421 tgctcgtgga gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca
      481 cagcactgtg caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag
      541 gttaattgat ggggttagcg gcaacgcgaa gctcttgatc gaagccccgg taaacggcgg
      601 ccgtaactat aacggtccta aggtagcgaa attccttgtc gggtaagttc cgacctgcac
      661 gaatggcgta atgatggcca ggctgtctcc acccgagact cagtgaaatt gaactcgctg
      721 tgaagatgca gtgtacccgc ggcaagacgg aaagaccccg
//
LOCUS       DAZGJT010000330          743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZGJT010000330 DAZGJT010000000
VERSION     DAZGJT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.337"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..701
                     /locus_tag="ACHKFK_005041"
     CDS             39..701
                     /locus_tag="ACHKFK_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO9055151.1"
                     /translation="MFIMTEFIKFKNSQSLRSFDKMVNIMQQKKMNLRKNKMEKGLSL
                     IEASMVLALSAIVVSGVMFYYQSASDSNKTQNTVSQVMSVMSAINGLYVGQASYTGLT
                     DAVLYSSSAVPENYKDKTNKQINNPFGGKLRVAPSGGGYTLSLDGVPKSSCVNIASMN
                     LGTSLQGVGVEVKSGTVLNPEALGSGKQPARGKVYSGSLTPANAAILCSDGPNSITFL
                     MK"
BASE COUNT          227 a          110 c          173 g          233 t
ORIGIN      
        1 tgttattagg ttggatggta ttaactttga aaatttgaat gtttatcatg acagagttta
       61 taaagtttaa aaattcacaa tcattaagga gttttgataa aatggtcaat ataatgcagc
      121 aaaaaaaaat gaatctaaga aaaaataaaa tggaaaaggg tctttcactt attgaagcat
      181 cgatggtgct ggctttgtct gcaatagttg tgtctggcgt tatgttttat taccaatcgg
      241 cttcggatag caataaaaca caaaacactg tatctcaggt gatgagtgtt atgtctgcta
      301 ttaacggttt gtatgtaggt caggcatcat atactggtct gactgatgct gtgctttata
      361 gttcttcggc tgttccggag aattataaag ataagaccaa caaacagatt aacaatccat
      421 ttggaggaaa attaagagtg gctccttctg gtggaggtta tactctttcc cttgatggag
      481 tgccaaaatc gtcatgtgtc aatattgctt caatgaacct tggcacttcc ttacagggtg
      541 tgggtgtgga ggtaaaaagc ggtactgttc taaacccaga agcactaggt agtgggaagc
      601 agccagcacg gggtaaggta tattcaggct cactgactcc tgctaacgca gctattcttt
      661 gttctgatgg gcctaacagt attacatttc ttatgaaata agttttatat agtttataag
      721 gaatattagg gaggggggag gga
//
LOCUS       DAZGJT010000331          740 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZGJT010000331 DAZGJT010000000
VERSION     DAZGJT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.338"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..377)
                     /locus_tag="ACHKFK_005042"
     CDS             complement(<1..377)
                     /locus_tag="ACHKFK_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO9055152.1"
                     /translation="MDMQKETIFSEVETANSKQIAVLKANFPQCFDKNGAFIQEKLLE
                     IVKSSDVELSKESYSLNWLGKSYARLLANLPPKTLLAEDKDHNQREENKNSQNLLIKG
                     DNLEVLKHMVNAYAEKVKMIYIDP"
     gene            complement(389..>740)
                     /locus_tag="ACHKFK_005043"
     CDS             complement(389..>740)
                     /locus_tag="ACHKFK_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055153.1"
                     /translation="PFYYAFSAIFDIVAVLNTEEIIMISGLNGGNFILSSMAKERVKS
                     GLLDRATIEGSKGEYTVIFDGSEWEGKPLVLSSSRQPYEARVFKSIDGAAAELLRIGL
                     SEASIKNTTKSNNK"
BASE COUNT          200 a          160 c          125 g          255 t
ORIGIN      
        1 ggatcaatat aaatcatctt caccttttca gcataagcat taaccatatg tttcaatact
       61 tcgagattat cccctttgat caatagattc tgactgttct tgttctcttc acgttggtta
      121 tggtctttat cttcggcaag caacgtcttc ggtggtaagt tagccaacag ccgcgcatag
      181 gacttaccca gccagtttaa gctgtatgac tctttagaga gttcaacatc cgatgatttg
      241 acgatctcaa gcaacttttc ttgaatgaaa gccccgtttt tatcaaaaca ctgcggaaag
      301 ttagccttta aaacagcaat ctgcttactg ttagcagttt caacttctga aaaaatcgtt
      361 tctttttgca tatccatttt aaacctgatt atttattgtt cgatttggtg gtgtttttga
      421 ttgaagcttc gcttaagcct atgcgtagta gttctgcagc tgcaccatct atgcttttga
      481 atactcgtgc ttcataaggc tgtctggagc tacttaagac taatggctta ccttcccact
      541 ctgaaccatc aaaaatcacg gtatactcgc ctttagaacc ttctattgtt gctctatcaa
      601 gtaaacctga tttgactctt tccttagcca tcgagctaag gatgaaattt ccaccattaa
      661 gacctgatat catgattatt tcctcggtat ttaatactgc tacgatatca aatatcgctg
      721 aaaaggcata atagaatggt
//
LOCUS       DAZGJT010000332          738 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZGJT010000332 DAZGJT010000000
VERSION     DAZGJT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.339"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>738
                     /locus_tag="ACHKFK_005044"
     CDS             <1..>738
                     /locus_tag="ACHKFK_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO9055154.1"
                     /translation="YGDPVDPGWSGFYWIRFVNAAGVKGPWHAVAGVAAQTQISVQAV
                     IDQIKEEAAKSPVIEELRKEIKNAQGQAVKDAAIKTTEVVGTLREETTRTIGGIETRI
                     STLDSSTSESLNEVDKRITKLDKEGGEAFLAMWSKKAGVDGITAGIGIVAGKDSEGRP
                     VSQVAISASQLFVFDPNNPDNTAYPFAVSGGKVVIPKAMIYNAVIETLVSRKVVADEV
                     KAGVSITSPVIRSAVIQNGNFQVDSQGN"
BASE COUNT          202 a          151 c          228 g          157 t
ORIGIN      
        1 tacggcgatc cggttgatcc gggatggtcg ggattttact ggatcaggtt tgtgaatgcc
       61 gcaggcgtta aagggccgtg gcacgcagtg gcaggcgtgg cagcacagac acagatatca
      121 gttcaggcgg tgattgacca gataaaagaa gaggcggcga agtctcccgt catagaagag
      181 cttcgcaagg aaataaaaaa cgcgcagggg caggctgtaa aggatgctgc aattaagaca
      241 accgaagttg tggggactct cagggaagaa acgacaagaa cgattggtgg tattgaaacc
      301 cgcattagca cactggattc gtcaaccagt gaatcgctta atgaggtcga caagcgcatc
      361 actaaactgg ataaagaagg cggtgaggcc ttccttgcga tgtggtcaaa aaaagcggga
      421 gttgatggca tcactgcggg gatcgggatt gtcgccggaa aagacagtga aggcaggcct
      481 gtaagtcagg ttgcaatttc tgcgtcgcag ctgtttgtct ttgacccgaa caacccggat
      541 aacaccgcct atccgtttgc ggtatcaggt ggcaaggtcg tgatcccgaa agcgatgatt
      601 tataacgcgg tgattgaaac actggtgtcg cggaaggttg tggcagatga agtaaaagcc
      661 ggggtaagta tcacttcgcc agttatccgg agtgccgtta ttcagaacgg aaactttcag
      721 gttgattctc agggtaac
//
LOCUS       DAZGJT010000333          731 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZGJT010000333 DAZGJT010000000
VERSION     DAZGJT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.340"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            43..>731
                     /locus_tag="ACHKFK_005045"
     CDS             43..>731
                     /locus_tag="ACHKFK_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO9055155.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          241 a          128 c          163 g          199 t
ORIGIN      
        1 atttaccagt aatgtaaata aattttcgag gagatcattc cagtgggacg taaatgggcc
       61 aatattgttg ctaaaaaaac ggctaaagac ggtgcaacgt ctaaaattta tgcaaaattc
      121 ggtgtagaaa tctatgctgc tgctaaacaa ggtgaacccg atccagaatt aaacacatct
      181 ttaaaattcg ttattgaacg tgcaaagcag gcacaagttc caaagcacgt tattgataaa
      241 gcaattgata aagccaaagg cggcggagat gaaacgttcg tgcagggccg ttatgaaggc
      301 tttggtccta atggctcaat gattatcgcc gagacattga cttcaaatgt taaccgtacg
      361 attgctaacg ttcgcacaat tttcaataaa aaaggcggca atatcggagc ggcaggttct
      421 gtcagctata tgtttgacaa tacgggtgtg attgtattta aaggatcaga ccctgaccat
      481 atttttgaaa ttttacttga agctgaagtt gatgttcgtg atgtgactga agaagaaggt
      541 aacattgtta tttataccga acctactgac cttcataaag gaatcgcggc tctaaaagca
      601 gctggaatca ctgagttctc aacaacagaa ttagaaatga ttgcgcaatc tgaagttgag
      661 ctttccccag aagatttaga aatctttgaa gggcttgttg atgcccttga agatgacgac
      721 gatgtacaaa a
//
LOCUS       DAZGJT010000334          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZGJT010000334 DAZGJT010000000
VERSION     DAZGJT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.341"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..608
                     /locus_tag="ACHKFK_005046"
     CDS             12..608
                     /locus_tag="ACHKFK_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HFO9055156.1"
                     /translation="MNNTDLIYLIKHFMHNELKAVEEVIYSPLSEFANLIKVLQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGDLGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFTRSHHSSLAISCDLSVEIPVKSEADNLGLAPSCSSTV
                     VLVVGDAVALALSELKKFTRADFGLYHPGGALGIKANS"
BASE COUNT          194 a          138 c          159 g          228 t
ORIGIN      
        1 ggagaataaa aatgaataac acggatctta tctatctcat taaacatttt atgcataacg
       61 aacttaaagc agtagaagag gttatttatt ccccactttc tgaattcgct aatttaatta
      121 aagtattaca atcttgtcag ggaaaagttg tttttattgg tgttggtaag tccggtatta
      181 ttgccagaaa actcgcagca acttttgcca gtaccggaac tccctcgttt tttgttcacg
      241 gtacggaagc ggtacacggc gaccttggaa tggtggcgaa agacgatgtt gttattctta
      301 tttcaaacag tggtgagacg gcggaaatcc tggcgacact gcctagtttg aaaaaaatgg
      361 gtaattattt gatctctttt acccgcagtc atcactcatc gcttgcgata agttgtgatt
      421 tatctgttga aatccccgtc aaaagtgagg cggataattt aggtttagca ccgtcatgct
      481 cgtcgactgt tgttctggtt gttggtgatg cggtggcgct tgcactctct gaattgaaaa
      541 aatttactcg tgctgatttc ggcttatatc atccaggagg tgcactcggc attaaagcaa
      601 attcataatt tcaaaatata aataaagtaa ccggtcacgc catcttttgg ggtggcgttc
      661 tttgaagtga gtgtccgcta tgaaaaatgt tctggtcacc gtccccagct tttcagcac
//
LOCUS       DAZGJT010000335          718 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZGJT010000335 DAZGJT010000000
VERSION     DAZGJT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.342"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            84..680
                     /gene="fimE"
                     /locus_tag="ACHKFK_005047"
     CDS             84..680
                     /gene="fimE"
                     /locus_tag="ACHKFK_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO9055157.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          198 a          136 c          175 g          209 t
ORIGIN      
        1 tttatctatt gggctaattt tgaccgattg aggtttccta taggtattca ttcaaatata
       61 tctcagtcag gagtactact attgtgagta aacgtcgtta tcttaccggt aaagaagttc
      121 aggccatgat gcaggcggtg tgttacgggg caacgggagc aagagattat tgtcttattc
      181 tgttggcata tcggcatggg atgcgtatta gtgaactgct tgatctgcat tatcaggatc
      241 ttgaccttaa tgaaggtcga ataaatattc gccgactgaa gaacggattt tctaccgttc
      301 acccgttacg ttttgatgag cgtgaagctg tggaacgctg gacccaggaa cgtgctaact
      361 ggaaaggcgc tgaccggacc gacgctatat ttatttctcg ccgcgggagt cggctttctc
      421 gccagcaggc ctatcgcatt attcgcgatg ccggtattga agctggaacc gtaacgcaga
      481 ctcatcctca tatgttaagg catgcttgtg gttatgaact ggcggagcgt ggtgcagata
      541 cccgtttaat tcaggattat ctcgggcatc gaaatattcg ccatactgtg cgttataccg
      601 ccagtaatgc tgctcgtttt gccggattat gggaaagaaa taatctcata aacgaaaaat
      661 taaaaagaga agaagtttga tttaacttat tgataataaa gttaaaaaac aaataaat
//
LOCUS       DAZGJT010000336          716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZGJT010000336 DAZGJT010000000
VERSION     DAZGJT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.343"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>716
                     /locus_tag="ACHKFK_005048"
     CDS             <1..>716
                     /locus_tag="ACHKFK_005048"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO9055158.1"
                     /translation="STLILTLILGFLIFSPSDLERLGKTMVYTLLSASNFFFMNGSGY
                     FDQSSETNPLLHTWSLAVEQQFYFLWPLIILAGFKLGKNKLKLFIFLIGVLSLVLSQL
                     TINSFPTANYYMPWFRAFEFAFGGIIFFIEKPKRKSYLNDILFVVGITLILYSAFSFG
                     KSTAFPGFNAMIPVIGACLSIRYGEKSNIGFILSNKLMVGIGVISYSVYLIHWPLMVF
                     YKYYVFRDISTYEMVFISVIP"
BASE COUNT          209 a          117 c          115 g          275 t
ORIGIN      
        1 tcgactctta ttctaacatt aattcttggc tttttgatat ttagtccaag cgatttagaa
       61 agactcggca aaacaatggt ttatacatta ttgtccgcgt caaacttttt cttcatgaat
      121 ggttctgggt attttgacca gtcatctgaa acaaacccac tacttcatac atggtctctt
      181 gctgttgagc aacaatttta tttcttgtgg cctttaataa ttcttgcggg ttttaaacta
      241 ggaaaaaaca agttaaaatt atttatattt ttgatcggtg ttttatccct agtcttatcg
      301 caactaacaa ttaactcatt ccctacagca aattattaca tgccatggtt tagggctttt
      361 gaatttgctt ttggaggaat aattttcttc atagaaaaac caaaaaggaa aagttattta
      421 aacgatatat tatttgtagt tggaatcacc ctcatattat atagtgcatt tagtttcggt
      481 aagtcaactg cgttccctgg attcaatgct atgatcccag ttattggcgc atgtctttct
      541 atacgatatg gtgaaaaatc aaatatagga tttattctat ccaataaatt aatggttggt
      601 ataggtgtaa tatcatattc tgtttacctt attcactggc cgttaatggt gttttataaa
      661 tattatgtat tccgtgatat ctctacgtat gaaatggtgt ttatatccgt aattcc
//
LOCUS       DAZGJT010000337          713 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZGJT010000337 DAZGJT010000000
VERSION     DAZGJT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.344"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>713
                     /locus_tag="ACHKFK_005049"
     CDS             <1..>713
                     /locus_tag="ACHKFK_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055159.1"
                     /translation="GAMAEDIKIGDNYIIRISENNESARLLDKSGKDALLGLINTKTG
                     AITTVNKTELEQKIKNTRGVEPQRIMESIRYTIDAPSLKKENINKLTSADLDKIKANV
                     EVVSKTITSETAPVYREAIQNGMTSEDALAVVNQDKTGGLLKQFNGLNTNVNALKNAT
                     TFAIDENGEITDGQGVETVSVKSVVADVKADTTIYQNKDGSYTLDQSAPGNVRVNDAV
                     VSLDNRTRSNTQAIQNHSR"
BASE COUNT          254 a          132 c          153 g          174 t
ORIGIN      
        1 ggggctatgg cagaggatat taaaattggt gacaattata ttattcgcat ttcagaaaat
       61 aatgaaagtg cacgtctgct tgacaaaagt gggaaagatg ctcttctggg acttattaat
      121 acaaaaacag gtgctattac tactgttaat aaaacagagc tcgaacaaaa aataaagaat
      181 actagaggcg ttgagccgca aagaattatg gagtctattc gttatacaat tgatgcgcca
      241 tctttaaaaa aagaaaatat taacaaatta acatctgccg atcttgataa aataaaggct
      301 aatgtagagg ttgtttcaaa aaccatcaca tcagaaaccg cacctgttta tcgcgaagct
      361 attcaaaacg gcatgacatc agaagatgcg ttggcagtgg taaaccagga taagacgggt
      421 ggattactga agcaattcaa cggtcttaat acaaatgtaa atgctctgaa aaatgccacc
      481 acgtttgcca ttgatgaaaa tggtgaaatt actgatggac agggcgttga aactgtatca
      541 gtgaagagcg ttgttgcaga tgtgaaagcc gatacaacaa tttatcagaa taaagatggc
      601 agctatacac ttgaccagag cgcacctggc aacgttcgtg tgaatgatgc tgttgtcagc
      661 ctcgacaaca gaacccgcag taacacccag gctatccaga atcactcccg cca
//
LOCUS       DAZGJT010000338          698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZGJT010000338 DAZGJT010000000
VERSION     DAZGJT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.345"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            67..450
                     /locus_tag="ACHKFK_005050"
     CDS             67..450
                     /locus_tag="ACHKFK_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaA family stress response protein"
                     /protein_id="HFO9055160.1"
                     /translation="MSVLYIQIRRNQITVRDLESKREVSGDAAFSNQRLLIANFFVAE
                     KVLQDLVLQLHPRSTWHSFLPAKRMDIVVSALEMNEGGLSQVEERILHEVVAGATLMK
                     YRQFHIHAQSVVLSDSAVLAMFKQK"
     gene            complement(514..>698)
                     /locus_tag="ACHKFK_005051"
     CDS             complement(514..>698)
                     /locus_tag="ACHKFK_005051"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9055161.1"
                     /translation="GRMLVKHALSMAPELTTNVNEQNEQAVGFYKKVGFKVTERSEVD
                     DLGKPYPLLNLAYVGV"
BASE COUNT          188 a          173 c          154 g          183 t
ORIGIN      
        1 aaaaaccccg gtcaataagg ccggggtttt ttgcatatca attatttgca tgatgaaggg
       61 aatctcatgt cagttctgta tatccaaatt cgtcgtaatc aaattactgt tcgcgatctt
      121 gaaagcaaac gtgaagtgtc aggagatgct gccttcagta accagcgcct gttaatcgcc
      181 aatttctttg ttgcagaaaa agttctgcaa gatcttgttc tgcaactcca cccacgttca
      241 acctggcatt cttttttgcc agcaaaacgt atggatattg ttgtgagcgc gctggaaatg
      301 aatgagggcg gtttgtcaca ggttgaggaa cgcattcttc atgaagtggt cgcaggggca
      361 acgttaatga aatatcgcca gttccacatc catgcgcaat cagtggtact cagtgatagt
      421 gcggtcctgg caatgtttaa gcagaaataa tcatgtcacc attggtgggt acgaaaccta
      481 aagttcagcc caccgggatg agaaaaaaac cgcctacacc cccacatatg ccagattcag
      541 caacggatac ggtttcccca aatcgtccac ctcagagcgt tccgtaacct taaagcccac
      601 cttcttatag aacccaaccg cctgctcatt ttgctcatta acattggttg tcagttccgg
      661 ggccattgag agcgcatgct tcaccagcat ccggccca
//
LOCUS       DAZGJT010000339          695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZGJT010000339 DAZGJT010000000
VERSION     DAZGJT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.346"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..66)
                     /locus_tag="ACHKFK_005052"
                     /pseudo
     CDS             complement(1..66)
                     /locus_tag="ACHKFK_005052"
                     /inference="COORDINATES: protein motif:HMM:NF013446.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            126..275
                     /locus_tag="ACHKFK_005053"
                     /pseudo
     CDS             126..275
                     /locus_tag="ACHKFK_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612150.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            302..406
                     /locus_tag="ACHKFK_005054"
                     /pseudo
     CDS             302..406
                     /locus_tag="ACHKFK_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          233 a          138 c          127 g          197 t
ORIGIN      
        1 cctcccgaac tcaaagctat catacagata attactggtt aaccctatat agggcatttt
       61 aaagtgttgt gatcttaccc agcaatagtg gacacgcggc taagtgagta aactctcagt
      121 cagaggtgac tcacaagaca aaacagtttc aaccagtaaa aaaacgtaaa caccattcgc
      181 atgaatttcg tagttaagat ctgaagcttg cagaatgcat cggtgttact gccggagtcc
      241 gtgaacttag tctgcatgaa tcaccacagc tttacatctg tcgcagtaaa cagcaaaatc
      301 agcaaactta cttaattata tcgagtgcga ttacaatcgc tggcgatggt acagttggtg
      361 tggcggtctc agtccggaac aatttgaaaa tcagaatctc acttaaaacg gcatccattt
      421 tacaaaagat ggccgtctgt gtatttgtaa tgaatacctt ctacatgttt agagtgcata
      481 atccacgaca aggcgatatc ctaaaaatta gctagcaggc aactttgatg gcatagataa
      541 tgtttttagt aaaaaagagt tgactgaata taatacagcc tcttcctaca taagaaggaa
      601 tgtgctcaca aaaaaaaacc attagtctgc ttcctgttct tacaataatc agtattatgt
      661 attttattta tatagcaaaa tatgcttatt acacc
//
LOCUS       DAZGJT010000340          661 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZGJT010000340 DAZGJT010000000
VERSION     DAZGJT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..661
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.347"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..323
                     /locus_tag="ACHKFK_005055"
     CDS             <1..323
                     /locus_tag="ACHKFK_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HFO9055162.1"
                     /translation="MEAANYTFEHNGRKWDYGKSTQTRLEPSVAAAKAGKLPEEFFWT
                     DADNNDVPMDAETLIALSAAAEQAMFTKGLEIHVRQRTMKKEIEALSGAEAILAYKVG
                     ITDG"
     gene            complement(446..>661)
                     /locus_tag="ACHKFK_005056"
     CDS             complement(446..>661)
                     /locus_tag="ACHKFK_005056"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055163.1"
                     /translation="RTKLFSAIPQPTKILYLMIVLFALFLCSHSVAYKKAPLSAPSSQ
                     YLCRWIKITINHKYHALGEHALPVFYL"
BASE COUNT          211 a          121 c          185 g          144 t
ORIGIN      
        1 cgatggaagc ggcgaactac acgtttgagc acaatgggcg taaatgggac tacgggaagt
       61 caacgcagac gcgtcttgag ccgtcggtgg cggcagcgaa agcggggaaa ctgccggagg
      121 agtttttctg gacggatgcg gacaataatg acgtaccgat ggacgctgaa acactgattg
      181 ctctgagtgc tgctgcagag caggcgatgt ttaccaaagg tctggaaatt catgtcagac
      241 agcgcacgat gaaaaaagag attgaagcgc tgagtggtgc ggaagctata ctggcgtata
      301 aagtcggtat cacagacggc tgataaaaaa gagaggggca agccatagcc ccttgctaca
      361 aatctacagc taaataatac cctggttgag gcaaattatt cggtaataaa aatttaagga
      421 ggcaaaatag cctccttcac ccctgtcaaa gataaaaaac ggggagtgcg tgctcaccga
      481 gagcatgata cttgtggttg atagttattt ttatccagcg acaaagatat tgagatgagg
      541 gcgcggatag tggtgctttt ttataagcaa cagaatggct gcataaaaag agagcaaata
      601 aaacaatcat caaatacaga attttcgtag gctgagggat tgctgaaaat aatttagtgc
      661 g
//
LOCUS       DAZGJT010000341          659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZGJT010000341 DAZGJT010000000
VERSION     DAZGJT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.348"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..292)
                     /locus_tag="ACHKFK_005057"
     CDS             complement(<1..292)
                     /locus_tag="ACHKFK_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055164.1"
                     /translation="MDDNNQTSGQPKPEPEECVKEQKITDHFKIMIDKARKAQKLVLI
                     KRADDLLRWGAQEEYDFSKIFGVKGNKEVNIRKYGHNTGRRMNARFLMMDGVR"
     gene            complement(282..>659)
                     /locus_tag="ACHKFK_005058"
     CDS             complement(282..>659)
                     /locus_tag="ACHKFK_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055165.1"
                     /translation="TPVPALQPAVLKTLGDEKSGDGSDSGEENEDSGGNCVTGSGGDD
                     RGDDEDEPEKYTLQFHFTDDDGIPYSETRYIAFFEDGTQTRGETDEEGYTERFFVSSK
                     HEIKVKLLFANDDFLSMEGHYGR"
BASE COUNT          156 a          185 c           97 g          221 t
ORIGIN      
        1 ttctcacacc atccatcata agaaatctag cattcatgcg cctacctgta ttatgtccat
       61 attttcttat attaacctcc ttatttcctt taacaccaaa gatcttactg aaatcatatt
      121 cctcctgtgc tccccatctc aaaagatcat ctgctctttt tatcaaaacc aacttttgtg
      181 ccttacgagc cttatcaatc attattttaa aatgatctgt tattttttgc tctttcacac
      241 attcttctgg ctctggcttt ggttgtccag aagtttgatt attatcgtcc atagtgtcct
      301 tccattgaga gaaaatcatc attcgcaaat agcaacttca ctttaatttc atgtttgcta
      361 gatacaaaaa aacgctctgt atacccctct tcatccgttt ctccgcgggt ctgcgtgcca
      421 tcctcaaaga aggcaatgta gcgggtttct gaatacggta tcccgtcatc gtctgtaaaa
      481 tggaactgga gagtatattt ttcaggttca tcctcatcgt cacctcgatc atcaccacca
      541 cttccggtca cgcagttccc tccagagtct tcattttctt ctccggaatc actgccatca
      601 ccactcttct catcccccag tgtcttcagc accgccggtt gcagcgccgg caccggcgt
//
LOCUS       DAZGJT010000342          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZGJT010000342 DAZGJT010000000
VERSION     DAZGJT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.349"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>658
                     /locus_tag="ACHKFK_005059"
     CDS             <1..>658
                     /locus_tag="ACHKFK_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9055166.1"
                     /translation="ESSGAETGPGVEVTKAKPVTRTITSANIDRLRVTFGVQSLVETT
                     SKGDRNPTSVRLLIQLERGGKWMTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVR
                     ETADSTTDQLQNKMLWSSYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGR
                     IIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKW
                     A"
BASE COUNT          153 a          175 c          204 g          126 t
ORIGIN      
        1 ttgagtcctc cggtgctgaa accggaccag gcgtggaagt gacgaaggca aagccggtga
       61 cgcgcaccat tacgtccgcg aacattgacc gcctgcgggt taccttcggg gtgcagtcac
      121 tggtggagac cacctcaaag ggtgaccgta acccgacgtc tgtccgcctg ctgattcagc
      181 ttgagcgtgg tggtaaatgg atgacggaaa aggatgtcac cattaacggc aagaccacct
      241 cgcagtttct ggcgtcggtg attctggata atctgcctcc ccgtcctttt aacatccgga
      301 tggtcaggga gacggcggac agcaccacgg accagctgca gaataagatg ctctggtcgt
      361 catacaccga aattatcgat gtgaaacagt gctacccgaa cacggccatt gtggggctgc
      421 aggtggatgc ggagcagttc ggcggccagc agatgacggt gaactaccat atccgcggtc
      481 gcatcatcca ggtgccgtca aactatgacc cggaaaaacg cacgtacagt ggtatctggg
      541 acggcagtct gaaaccggca tacagtaaca atccggcctg gtgtctgtgg gacatgctga
      601 ctcacccgcg ctacggcatg ggaaaacgtc tgggggcggc ggatgtggac aagtgggc
//
LOCUS       DAZGJT010000343          603 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZGJT010000343 DAZGJT010000000
VERSION     DAZGJT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.350"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..320
                     /locus_tag="ACHKFK_005060"
     CDS             81..320
                     /locus_tag="ACHKFK_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055167.1"
                     /translation="MSQYPELIAQFSSGNQTRIKQGLIAKAPLEGWHYGTKEIVKKFH
                     MYHRVAIEYSGGIYDIDNLRRAHLICMMKFITGDS"
     gene            321..>603
                     /locus_tag="ACHKFK_005061"
     CDS             321..>603
                     /locus_tag="ACHKFK_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO9055168.1"
                     /translation="MYNFKDEIEDYTEREFIELLGEFTNPTGDNAQLKGEELDKYWDD
                     LEEHLTRITQHPLMSDLIYYPAKKGDDEPENILKIVKEWRRSQGLPLFKD"
BASE COUNT          209 a          100 c          130 g          164 t
ORIGIN      
        1 taaaaaacag gtaaaaatac tgaagcctca ttaggttatt ttatcatttt cccgctttta
       61 ccgataccct ttggctggag gtgagtcagt atccggagtt gatagcgcag ttttcaagcg
      121 gtaatcaaac acgtataaaa caaggattaa tcgcaaaagc accacttgaa ggttggcatt
      181 atggcactaa agaaatcgtt aaaaaattcc acatgtatca tcgtgttgcg attgaatata
      241 gtggcggaat ttacgatatt gataatttaa ggagggcaca cctcatttgc atgatgaaat
      301 ttattacagg agatagttaa atgtataatt ttaaagatga aatagaagat tacacagagc
      361 gggaattcat tgagttgctt ggcgagttta caaatccaac cggggataac gctcaattaa
      421 aaggggaaga gcttgacaaa tattgggatg atctagaaga acatcttacc agaattacac
      481 aacatccgtt gatgagcgat cttatctact atcctgcaaa gaagggagat gatgaacctg
      541 aaaacatcct gaaaattgtc aaagaatggc ggcgttcaca ggggctaccc ttgtttaaag
      601 act
//
LOCUS       DAZGJT010000344          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZGJT010000344 DAZGJT010000000
VERSION     DAZGJT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.351"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>584
                     /locus_tag="ACHKFK_005062"
     CDS             <1..>584
                     /locus_tag="ACHKFK_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9055169.1"
                     /translation="TDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQIINDEL
                     YLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLW
                     HAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICW
                     HKFARYWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          153 a          143 c          152 g          136 t
ORIGIN      
        1 acggatttcc gctcagaact actcgattca cgttttggcg caaaggccat ttctactatc
       61 gcggagtcaa aacgatttcc gctgcacgaa atgcgcgatg atgttgcatt tcagattatc
      121 aatgatgaat tatatcttga tggcaacgct cgtcagaacc tggccacttt ctgccagacc
      181 tgggacgacg aaaacgtcca taaattgatg gatttgtcga tcaataaaaa ctggatcgac
      241 aaagaagaat atccgcaatc cgctgcaatc gacctgcgtt gcgtaaacat ggttgccgat
      301 ctgtggcatg cgcctgcacc gaaaaatggt caggctgttg gcaccaacac cattggttct
      361 tccgaggcct gtatgctcgg cgggatggcg atgaaatggc gttggcgcaa gcgtatggaa
      421 gccgcaggca aaccaacgga taaaccaaac ctggtgtgcg gtccggtaca aatctgctgg
      481 cataaattcg cccgctactg ggatgtggag ttgcgtgaga tccctatgcg ccccggtcag
      541 ttgtttatgg acccgaaacg catgattgaa gcctgcgacg aaaa
//
LOCUS       DAZGJT010000345          584 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZGJT010000345 DAZGJT010000000
VERSION     DAZGJT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.352"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>584
                     /locus_tag="ACHKFK_005063"
     CDS             <1..>584
                     /locus_tag="ACHKFK_005063"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9055170.1"
                     /translation="NDCLSSINSQILKPNQIVIVYDGPIRKELDDIVTRWEKQLPIHC
                     VKLTSCTGLGNALNYGLKFCDYDIVCRMDTDDICRNDRFLRQVKILESDNNLALIGSY
                     ISEFDSTPSNSHAVRTVPIEHNDICLYAKKRNPFNHMTVAFRKSVVTSLGGYKDEYLY
                     EDYALWIKMLKNGFLTKNIPEALVYARVGNNMEL"
BASE COUNT          198 a           78 c          118 g          190 t
ORIGIN      
        1 ttaatgattg cttaagtagc attaattcgc aaatattaaa accgaaccaa attgtaatcg
       61 tatatgatgg cccaattaga aaagaattgg acgatatagt tacaagatgg gaaaagcaat
      121 tgccaataca ttgtgtaaag ctcacttcct gtacaggatt aggtaatgca ttgaattatg
      181 gtttgaaatt ttgtgattac gatattgtct gtcgtatgga cactgatgat atatgtagaa
      241 atgacagatt tttaagacaa gttaaaatac ttgaaagtga taataatctg gctttgattg
      301 ggtcttatat ctcagagttt gactctactc cctcaaacag tcatgctgtt aggaccgttc
      361 ccattgagca taatgatatt tgtttatatg caaaaaaaag aaatccgttt aaccatatga
      421 ctgtagcatt tagaaaaagt gtagtaactt ctctcggtgg gtataaggat gaatatttat
      481 atgaagacta cgcactgtgg attaagatgc ttaaaaatgg atttttgact aaaaatattc
      541 cagaggcgct agtttatgcg cgggttggta ataatatgga gctt
//
LOCUS       DAZGJT010000346          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZGJT010000346 DAZGJT010000000
VERSION     DAZGJT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.353"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            189..368
                     /gene="yciG"
                     /locus_tag="ACHKFK_005064"
     CDS             189..368
                     /gene="yciG"
                     /locus_tag="ACHKFK_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO9055171.1"
                     /translation="MTEYRGGSGNFAEDREKASNAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            454..>571
                     /locus_tag="ACHKFK_005065"
     CDS             454..>571
                     /locus_tag="ACHKFK_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HFO9055172.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEK"
BASE COUNT          197 a          120 c          110 g          144 t
ORIGIN      
        1 taattttctc taagtcactt attcctcagg caattgttaa tacatccaga acgttcctca
       61 aaatatattt ttccactttc ttctcgttac gcttaatttg actaattctc attaacgact
      121 aattttaatg agtgtcgaca cacaacactc atattaatga aacaatgcaa cgcaacggga
      181 gaaataacat gaccgaatat cgtggtggtt caggaaattt cgccgaagac cgtgagaagg
      241 catccaacgc aggccgtaaa ggcggtcagc atagcggcgg taattttaaa aatgatccgc
      301 aacgcgcatc cgaagcaggt aaaaaaggcg gtcaacaaag cggtggtaat aaatcaggaa
      361 aatcctgatt caccgttaat tattaatatc agctgataaa gataattgcc tctgcgggcc
      421 gtatggctcg cagtgatttc actggagaaa aatatgaata tgaagaccat tgaagatgta
      481 tttattcacc tgctttcaga tacctacagc gcagaaaaac aattaacccg ggcactggca
      541 aaactcgcaa gagcaacatc aaacgaaaaa t
//
LOCUS       DAZGJT010000347          570 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZGJT010000347 DAZGJT010000000
VERSION     DAZGJT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.354"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>570
                     /locus_tag="ACHKFK_005066"
     CDS             <1..>570
                     /locus_tag="ACHKFK_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO9055173.1"
                     /translation="ELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQIINDELYLDGN
                     ARQNLATFCQTWDDDNVHKLMDLSINKNWIDKEEYPQSAAIDLRCVNMVADLWHAPAP
                     KNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTNKPNLVCGPVQICWHKFAR
                     YWDVELREIPMRPGQLFMDPKRMIEACDE"
BASE COUNT          148 a          136 c          148 g          138 t
ORIGIN      
        1 ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag aatcaaaacg
       61 ttttccgctg cacgaaatgc gtgatgatgt cgcattccag attatcaatg atgaattata
      121 tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg acgacgataa
      181 tgtccataag ttgatggatt tatccattaa caaaaactgg atcgacaaag aagaatatcc
      241 gcaatctgca gcaatcgatc tgcgttgcgt aaacatggtt gccgatctgt ggcatgcgcc
      301 tgcaccgaaa aatggtcagg ccgttggcac caacaccatt ggttcttccg aggcctgtat
      361 gctcggcggg atggcgatga aatggcgttg gcgcaagcgt atggaagctg caggtaagcc
      421 gactaacaaa ccgaacctgg tgtgcggtcc ggtacaaatc tgctggcata aattcgcccg
      481 ctactgggat gtggagttgc gtgagatccc tatgcgcccc ggtcagttgt ttatggaccc
      541 gaaacgcatg attgaagcct gcgacgaaaa
//
LOCUS       DAZGJT010000348          569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZGJT010000348 DAZGJT010000000
VERSION     DAZGJT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.355"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..569
                     /locus_tag="ACHKFK_005067"
                     /pseudo
     CDS             1..569
                     /locus_tag="ACHKFK_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006663458.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
BASE COUNT          161 a          100 c          129 g          179 t
ORIGIN      
        1 aaatatcatt tgcgtgtaga tgagaagaaa tctcgaaatg tattcagcgc catacgtgat
       61 gagctcatcc ttgatgggga ttcacgacaa aacttagcta ctttctgtca aacatggatt
      121 gatgatgaaa tccgtgagtt gcttaattta tctattgata gaaacatgat ttataaagat
      181 gaatatccac aaatggcgga acctgaaggt cgctgtgtgc gtatgctttc cgattcgtgg
      241 agtttccccg attcgcgaca caccttaggt tgctcaacca ttggttcatc tgaagctgct
      301 atgctcggcc gcttggcgtt aaaatggcag tggtgtaaaa aacgcgaagt acaaggtaaa
      361 tcaaccgaac ctgatttgcg gtcctgtgca aatatgctgg cataaattct gccgttactg
      421 tgatgtgaaa ttgcgtaaaa tttcacttta tgatcatcgc ctgataatga atgctgaaga
      481 ggttttgaag catgtcgatg aaaacactat tggtgtcgtt acgactttag gtattacttg
      541 tttttatttt tgtaaagtag attgcgtta
//
LOCUS       DAZGJT010000349          556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZGJT010000349 DAZGJT010000000
VERSION     DAZGJT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.356"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..149
                     /locus_tag="ACHKFK_005068"
     CDS             <1..149
                     /locus_tag="ACHKFK_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9055174.1"
                     /translation="IFTQCIERNNLTLRTRIKRLGRKTICFSRSVEIHEKVIGAFIEK
                     HMFY"
     gene            complement(268..>556)
                     /locus_tag="ACHKFK_005069"
     CDS             complement(268..>556)
                     /locus_tag="ACHKFK_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO9055175.1"
                     /translation="NHLISLHPVNEIILGCTHYPIVQNLFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNPSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
BASE COUNT          163 a          102 c          118 g          173 t
ORIGIN      
        1 aaatattcac ccaatgcatt gagcgtaata atctgacgct acgcacccgc attaagcggt
       61 tgggtcgtaa aacaatctgc ttctcacgct cagttgagat ccacgaaaaa gtgatcggag
      121 cgtttatcga aaaacacatg ttctactaat tggatgcact acccagtgtg taactgggtt
      181 tgcccataaa atgcacctta ctcagttggt gtccaacttt tggggtgcag ttcagaagga
      241 cggggtttta cggtgcaccg gataaattta aaactgcctg atactgaatg tatgaggttt
      301 cgtaatgctc aattccccta gtaccgtttt gtagatctcc atacttccac tagtatttat
      361 ttgtaaagtt cctatatttg aggggtttaa taaatttagc tgtcccaagt gatttttaat
      421 agagatagcc tggtcatgtg caggattaat aaattttata tccggtgcaa ctgcttcaaa
      481 taaattctga acaattggat agtgagtaca gccgagaata atttcattta ctggatggag
      541 ggaaattaaa tggtta
//
LOCUS       DAZGJT010000350          555 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZGJT010000350 DAZGJT010000000
VERSION     DAZGJT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.357"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            278..>555
                     /locus_tag="ACHKFK_005070"
     CDS             278..>555
                     /locus_tag="ACHKFK_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="activates the csgBA and csgDEFG operons involved in
                     biofilm formation; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HFO9055176.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPED"
BASE COUNT          180 a           95 c           93 g          187 t
ORIGIN      
        1 accaaatgta caacttttct atcatttata aacttaataa aaccttaagg ttaacatttt
       61 aatataacga gttgcattta gttacatgtt taacacttga tttaagattt gtaatggcta
      121 gattgaaatc agatgtaatc cattagtttt atattttacc catttagggc tgatttatta
      181 ctacacacag cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg
      241 tcaggtgtgc gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat
      301 tcatggtcat acattattgt tgatcactaa accttctttg caggcgacag ctctcttgca
      361 gcaccttaaa caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga
      421 cgatatatct tcaggctcta ttattcttct ggatatgatg gaagcggata aaaagcttat
      481 ccattattgg caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa
      541 tacgcctgaa gatta
//
LOCUS       DAZGJT010000351          546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZGJT010000351 DAZGJT010000000
VERSION     DAZGJT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.358"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(425..>546)
                     /gene="gspM"
                     /locus_tag="ACHKFK_005071"
     CDS             complement(425..>546)
                     /gene="gspM"
                     /locus_tag="ACHKFK_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO9055177.1"
                     /translation="LNWLNALDEKYALRVTQIDVSAAEKPGMVNVQRLEFGRG"
BASE COUNT          159 a          123 c           99 g          165 t
ORIGIN      
        1 atacctgagc agacatttct taaaatcgac gaatagtttc cctgggaatt attatgctta
       61 tttgaaattc cctttctgat tatctccagt aattttacta atttccaggc tacataaaat
      121 acagatcaca taagaattat cctatatgtt ttgacctatc aattgttact caatgattta
      181 ttacaactat taataatcat aatgtgtcaa aacacttaag catctttaca gtcttagaaa
      241 gatgtcgatt aacgaaggct acatggtgaa aaacaggttc cttcagtgtt accgaatatt
      301 ccgtggtggt ctggagggag ataattcttg ctacatcgaa actcagcccg gtgagcgggt
      361 ttgacagttc cgcactcacc attgggctaa gggttatcag gtggggttaa ggaaatggca
      421 atacctaccc ccgcccaaac tccagtcgct gcacattcac catcccaggc ttctcagcag
      481 cactgacatc aatttgtgtc acccgcagcg catatttttc atccagtgcg tttaaccagt
      541 tcagca
//
LOCUS       DAZGJT010000352          545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZGJT010000352 DAZGJT010000000
VERSION     DAZGJT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.359"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>545
                     /locus_tag="ACHKFK_005072"
     CDS             <1..>545
                     /locus_tag="ACHKFK_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055178.1"
                     /translation="KDVDQRNFDYRAKVDDRNYALQEREFNANQNYRNASLGMEQQRL
                     RMQKYNQRRLEYNDMLARDQPVMAALGKAVDAGDRDAAMRLYGQLSEGNPLRLMANDG
                     YVAKAGQAVNNLQKIFDDKPDRAIASLNTPENLDVLSGVFGPELQQRIGMPDSTGKKT
                     IKEARIGSIVPAQQEGYILIGL"
BASE COUNT          149 a          121 c          165 g          110 t
ORIGIN      
        1 aaagacgttg atcagcggaa ctttgattac agagccaaag tcgacgaccg taattatgca
       61 ctgcaggaaa gggagtttaa cgccaaccag aattaccgga atgcgtcgct gggtatggag
      121 cagcagcgac tccggatgca gaaatacaac cagcgacggc ttgagtataa cgatatgctg
      181 gcgcgcgatc agcctgtgat ggcggcgctt ggaaaggctg tggatgctgg cgatcgggat
      241 gcagcaatgc gtctttacgg ccagttgtca gagggtaatc cgctgaggct gatggcgaat
      301 gatggctatg tagcgaaagc gggtcaggcc gtgaacaacc tgcaaaaaat ctttgatgac
      361 aagccagaca gggctatcgc ttcacttaat acgccggaga atcttgatgt cctttccggg
      421 gtgtttggcc cggaactgca acagcgtatt ggcatgcccg attcaaccgg gaaaaagacg
      481 ataaaagagg ccaggattgg cagtatagta ccggcacagc aggaagggta catacttatc
      541 ggcct
//
LOCUS       DAZGJT010000353          543 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZGJT010000353 DAZGJT010000000
VERSION     DAZGJT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.360"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>543
                     /locus_tag="ACHKFK_005073"
     CDS             <1..>543
                     /locus_tag="ACHKFK_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO9055179.1"
                     /translation="KLYGVGPVCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLKK
                     EILRVYDGNHQVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTVSR
                     KAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGVVYVAFIIDVFAGYIVGWRVSSSM
                     ETTFVLDALEQALWARRPSGT"
BASE COUNT          116 a          130 c          173 g          124 t
ORIGIN      
        1 taagctgtac ggggtcggac cggtatgcag tgaactacat attgccccgt caacgtatta
       61 ccactgtcag caacagcgac atcatcctga taaacgcagt gcccgtgcgc agcgcgatga
      121 ctggctgaag aaagagatac tgcgcgtata cgatgggaat catcaggtat acggtgtgcg
      181 taaagtctgg cgtcagttgt tacgggaagg aatcagggta gccagatgta cagtggcgcg
      241 cctcatggcg gttatgggac ttgccggtgt tctccggggt aaaaaggtcc gcactaccgt
      301 cagccggaaa gccgttgccg caggcgaccg cgtaaaccgt cagtttgtgg cagaacgtcc
      361 tgaccagttg tgggtggctg attttactta cgtcagcaca tggcaggggg tcgtctatgt
      421 ggcgttcatc attgatgtgt ttgccggata catcgtgggg tggcgggtct catcgtctat
      481 ggaaacgaca ttcgtgctgg atgcactgga gcaggcgtta tgggcccgtc gaccgtccgg
      541 cac
//
LOCUS       DAZGJT010000354          528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZGJT010000354 DAZGJT010000000
VERSION     DAZGJT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.362"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..110
                     /locus_tag="ACHKFK_005074"
     CDS             <1..110
                     /locus_tag="ACHKFK_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9055180.1"
                     /translation="IYDGGRDCRNRSNNCYGQVSRRDQESALACWGIDR"
     gene            267..449
                     /locus_tag="ACHKFK_005075"
     CDS             267..449
                     /locus_tag="ACHKFK_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055181.1"
                     /translation="MEKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            464..>528
                     /locus_tag="ACHKFK_005076"
     CDS             464..>528
                     /locus_tag="ACHKFK_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055182.1"
                     /translation="MSDKLITLAKILCVIVGISFSL"
BASE COUNT          147 a           98 c          153 g          130 t
ORIGIN      
        1 ggatatatga cggcggcagg gactgtcgga atcgctcaaa taactgttac gggcaggtat
       61 cgcggcgtga ccaggagagc gcgctggcgt gctggggaat tgacagataa gcagaatatt
      121 ttgctgaaaa atgcggtttg ctcacacgga cggataacac gaaatcctgc gaactgacaa
      181 aaactaagtg aataaaagta aaaaccccgt ttgttggctg caagcggggt tttgtgtttc
      241 tgaccttgga taaggcaagg gagaacatgg aaaagtataa acgaattctg ttgaggttga
      301 ctatgaaaaa tggccttgaa ctgaaagcgc ctgtaactga tgacatcagc agagcactgg
      361 cttttgccat taagtgggtg gcggtcggtg ttgctgtgtc cccgatgctg tatgggctgg
      421 caaaactggt cattgcgttg aaatcgtgaa gggaggatta agcatgtcag acaaactcat
      481 aacgctggcg aagatcctct gtgtaattgt cggcatttca ttttcact
//
LOCUS       DAZGJT010000355          521 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZGJT010000355 DAZGJT010000000
VERSION     DAZGJT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.363"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..521
                     /locus_tag="ACHKFK_005077"
                     /pseudo
     CDS             1..521
                     /locus_tag="ACHKFK_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1-like element IS1A family transposase"
BASE COUNT          122 a          128 c          158 g          113 t
ORIGIN      
        1 gatatggcca tgaatggcgt tggatgccgg gcaactgccc gcattatggg cgttggcctc
       61 aacacgattt tacgtcactt aaaaaactca ggccgcagtc ggtaacctcg cgcatacagc
      121 cgggcagtga cgtcatcgtc tgcgcggaaa tggacgaaca gtggggctat gtcggggcta
      181 aatcgcgcca gcgctggctg ttttacgcgt atgacagtct ccggaagacg gttgttgcgc
      241 acgtattcgg tgaacgcact atggcgacgc tggggcgtct tatgagcctg ctgtcaccct
      301 ttgacgtggt gatatggatg acggatggct ggccgctgta tgaatcccgc ctgaagggaa
      361 agctgcacgt aatcagcaag cgatatacgc agcgaattga gcggcataac ctgaatctga
      421 ggcagcacct ggcacggctg ggacggaagt cgctgtcgtt ctcaaaatcg gtggagctgc
      481 atgacaaagt catcgggcat tatctgaaca taaaacacta t
//
LOCUS       DAZGJT010000356          519 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZGJT010000356 DAZGJT010000000
VERSION     DAZGJT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.364"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>519
                     /locus_tag="ACHKFK_005078"
     CDS             <1..>519
                     /locus_tag="ACHKFK_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055183.1"
                     /translation="EKVVLGAINTTVGSVMTWNKQDAEKVQKIIEKLPEKQQQVGTQI
                     AMQRIVFPVDSPSLKLDKLKSGTLTKADIDAVVSLKTDVSNAISIETAPAYVSAVRSG
                     GNSDAALAAAKHGPGALQEYNRIVTNIDRLNKDTTFAIDANGNITLDETGSVERESVK
                     NVVAAIQADTTI"
BASE COUNT          172 a           96 c          115 g          136 t
ORIGIN      
        1 tgaaaaagtt gtattaggtg ctataaatac aactgtaggt agtgtcatga cttggaacaa
       61 acaggatgct gaaaaagttc aaaaaatcat tgaaaaattg ccagaaaaac aacaacaagt
      121 tggtacacaa attgcgatgc aacgaattgt gttcccggtt gattctccta gtttaaagct
      181 tgataagttg aagtcaggaa cattaaccaa ggcggatatt gatgctgttg tatccttaaa
      241 aacagatgtt tcaaatgcta tttctatcga aacagcgcca gcgtatgtgt ctgcggttcg
      301 ttcaggggga aactctgatg ccgcgcttgc tgctgctaaa cacggcccag gtgctctgca
      361 ggaatacaac cgcatcgtta ccaatataga taggttgaat aaagacacaa cttttgctat
      421 cgatgctaac ggtaacatta cccttgatga aacaggctca gtggagcgag agtctgtaaa
      481 aaatgtagtt gcagccattc aggcagatac aacaattta
//
LOCUS       DAZGJT010000357          517 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZGJT010000357 DAZGJT010000000
VERSION     DAZGJT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.365"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>517)
                     /locus_tag="ACHKFK_005079"
     CDS             complement(<1..>517)
                     /locus_tag="ACHKFK_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO9055184.1"
                     /translation="IKELLDYQFSTNEIAAVVAERAVEWWQNRASVLTTPQLASGYFN
                     AGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISGKYNT
                     RYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDL
                     LKPVNSNQYRYC"
BASE COUNT          161 a          107 c           82 g          167 t
ORIGIN      
        1 cacaataccg atactgattg ctatttacag gtttaagtaa atcttcttta ctccacggag
       61 aagctgcttt ggcgatcaaa aaactacgtg acaccggata atcagcccac tcatgccagg
      121 gttttgtagg tcccacatag tgtataaaca cggtgtcatc attgactgga ttatcaactt
      181 tgtcttttaa ttcatagtta atactatatc gggtattata tttccccgaa ataaacttca
      241 ctttaccatt caataataca ttcagtacat cttgatcaag gtgggtgatt ttacttaccc
      301 aatctgggtc acgcaacatt tcaatagctt ttgacgaaat gttatttaga ttccactcat
      361 caatatttat cagtaaaaaa ccagcattga aatatccaga agctaattgt ggtgtagtta
      421 ataccgaggc tcgattctgc caccattcaa cagctctttc agccacaact gcggcaattt
      481 cattagtaga aaattgataa tctaagagtt ctttaat
//
LOCUS       DAZGJT010000358          513 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZGJT010000358 DAZGJT010000000
VERSION     DAZGJT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.366"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>513
                     /locus_tag="ACHKFK_005080"
     rRNA            <1..>513
                     /locus_tag="ACHKFK_005080"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          112 a          111 c          169 g          121 t
ORIGIN      
        1 tgtaggatag gtgggaggct ttgaagtgtg gacgccagtc tgcatggagc cgaccttgaa
       61 ataccaccct ttaatgtttg atgttctaac gttggcccct aatcggggtt gcggacagtg
      121 tctggtgggt agtttgactg gggcggtctc ctcctaaaga gtaacggagg agcacgaagg
      181 ttggctaatc ctggtcggac atcaggaggt tagtgcaatg gcataagcca gcttgactgc
      241 gagcgtgacg gcgcgagcag gtgcgaaagc aggtcatagt gatccggtgg ttctgaatgg
      301 aagggccatc gctcaacgga taaaaggtac tccggggata acaggctgat accgcccaag
      361 agttcatatc gacggcggtg tttggcacct cgatgtcggc tcatcacatc ctggggctga
      421 agtaggtccc aagggtatgg ctgttcgcca tttaaagtgg tacgcgagct gggtttagaa
      481 cgtcgtgaga cagttcggtc cctatctgcc gtg
//
LOCUS       DAZGJT010000359          505 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZGJT010000359 DAZGJT010000000
VERSION     DAZGJT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.367"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          178 a           81 c           94 g          152 t
ORIGIN      
        1 ttattgtttc attggtaata atcacaagaa gaaatagata ccttaggtat agtcagatgg
       61 aacaaggatt ttcaaaataa aatatatatg gtattaatat ttcccgatct gtattcaatg
      121 attcgctcaa agattgaaat tcaagagaaa ttgttatagc agtgatacga aacaacaaaa
      181 cggaatgatt gccagccgtt agaaaacacc ggacactctc gtgtaaaaag ctaccctatc
      241 tatggatata gcgatatcaa ctcatcaagc gtggactgaa tgatattcag ctccatatgg
      301 gcggcaacct ggaggataaa catgatttcg ccgttggaat caaggttgtt ctgcaatatc
      361 taccagactg cttcaattta tctgttttgg cagtgccata atcttaattt aactatttga
      421 agatgatgat attgtaagat gccagaaatt tagtaatctg gagaaccatg tttacgatca
      481 ctaaatatta aagtaatagt cgata
//
LOCUS       DAZGJT010000360          494 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZGJT010000360 DAZGJT010000000
VERSION     DAZGJT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.368"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(105..281)
                     /locus_tag="ACHKFK_005081"
                     /pseudo
     CDS             complement(105..281)
                     /locus_tag="ACHKFK_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(290..412)
                     /locus_tag="ACHKFK_005082"
                     /pseudo
     CDS             complement(290..412)
                     /locus_tag="ACHKFK_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(429..>494)
                     /locus_tag="ACHKFK_005083"
     CDS             complement(429..>494)
                     /locus_tag="ACHKFK_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO9055185.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          125 a           99 c          109 g          161 t
ORIGIN      
        1 ggtcatgata gatgtgtttc aatgccaacc tggtctattc ctacatatgt gagagcatat
       61 ttacaaaaaa atcgttattg tgatttcaac ggtgatgcat ccaattagta gaacatgcat
      121 ttttccatga atgccccgat gactttttca tgaagctcaa tagaacgtga aaaatagagc
      181 gttttgcggg ccagccgttt gattctggtt ctcagcgtca ggttattgcg ctcaatgcgc
      241 tgagtaaaaa tcttgccggt caggtgcttt tctttcggaa gtgaagtggt caacaaaaac
      301 tggccaccgc gttagagttt ttccagtatc gattttccga ttcgtttggg ggtaacccac
      361 cattatattc gtgcggtctt agtgcgctgt aatatccaac gatatagtcc gttggtaatg
      421 actccaactt attgatagtg ttttatgttc agataatgcc cgatgacttt gtcatgcagc
      481 tccaccgatt ttga
//
LOCUS       DAZGJT010000361          490 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZGJT010000361 DAZGJT010000000
VERSION     DAZGJT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.369"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            302..>490
                     /locus_tag="ACHKFK_005084"
     CDS             302..>490
                     /locus_tag="ACHKFK_005084"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055186.1"
                     /translation="MFFSLSVLNLEDHLGLVISPQSPLSAGYITLRACRRSLPLRFST
                     VTPGGIVGFLIVSYRFNIP"
BASE COUNT           90 a          155 c          120 g          125 t
ORIGIN      
        1 ccagcgcgtc atcggagcca tgccccctta acatccgtcg ctgaccttcc ccggtttacc
       61 tgcctggact caggcatact gaggaggctg cccgtcgcac tctttatcct tgcttgccgc
      121 aagttggcag aagtcagcaa cgcaagcgtg atagacgctg ctgccccggt gtttcgcata
      181 catgtcaaaa caccttcgac cggcagtgct tacgtatcac tgccagttcc tcctgcacgg
      241 cctgtcagat cacgtaggcc gtggtgacgt tttcaaccca cagaggcgga ttaacgggtt
      301 catgttcttc agcctttcag tacttaacct tgaggatcat ctcggcttag tgatctcgcc
      361 tcaatccccg ttgtcagcgg gttacatcac cctgcgggca tgccgcaggt cactgccgct
      421 caggttctcc accgtcacac ccggtgggat tgttgggttt ctcatcgtga gttaccggtt
      481 caatattcca
//
LOCUS       DAZGJT010000362          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZGJT010000362 DAZGJT010000000
VERSION     DAZGJT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.370"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>484
                     /gene="wzy"
                     /locus_tag="ACHKFK_005085"
     CDS             <1..>484
                     /gene="wzy"
                     /locus_tag="ACHKFK_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033808138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="O107/O117 family O-antigen polymerase"
                     /protein_id="HFO9055187.1"
                     /translation="WGLIFSMQYSVSARDNIEMGSNIITTFLKQQGVSVNVIGIAIKD
                     KNNSLLSESVILSPLYDSAISLANSLVGVQSNGNSVEFAENSFSLSHKLSYLEDPSAY
                     LAGYGVGGAAIAELYIVGGYLACLIGGMLTYIFISILEKIAKKSFFNFIFVMLITGKI
                     "
BASE COUNT          143 a           67 c           97 g          177 t
ORIGIN      
        1 tttggggttt aatatttagt atgcaatact cggtttcagc aagagataat attgaaatgg
       61 ggtcgaatat catcacaacg ttcttaaaac aacaaggcgt atcagtcaat gttattggta
      121 ttgctataaa ggacaaaaat aattcattat taagtgaatc tgtgattttg tcgccactat
      181 atgatagtgc tatttcttta gctaattctt tagtaggtgt ccaaagcaat ggaaattcgg
      241 tagagttcgc agagaattca ttctcattgt cgcacaaatt gagttattta gaagatcctt
      301 cagcatacct tgctggctat ggtgttgggg gagccgctat tgctgagctg tacatagttg
      361 gtggttactt ggcttgttta attggaggta tgcttacata tatttttatc tctatactag
      421 aaaaaatcgc caaaaaatcc ttttttaatt ttatttttgt tatgttgata actgggaaaa
      481 tatt
//
LOCUS       DAZGJT010000363          475 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZGJT010000363 DAZGJT010000000
VERSION     DAZGJT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.371"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..>475
                     /locus_tag="ACHKFK_005086"
     CDS             114..>475
                     /locus_tag="ACHKFK_005086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055188.1"
                     /translation="MEMKKERGFSLVEIALFSVLIGVVIANIYDIKRTFVKNIYHKQL
                     SLAICSYANAFSRYINMGENITGNITIEQLKNKGVITPFAEPQIGYFTVSFQIIKKNG
                     DRYGLMKLHSNKKITIEE"
BASE COUNT          178 a           69 c           84 g          144 t
ORIGIN      
        1 accaatcaag atagtgccat aataaactat caagcgcttg ataagcttga taatcaaata
       61 aaacataact gttttcgtac acgtcaagat gataaccttg ttctgggggt ggtatggaaa
      121 tgaaaaaaga acgtggtttt tcgttggtag agattgcact tttctctgtt ttgataggcg
      181 tggtaatagc taatatatat gatataaaaa gaacttttgt taagaatatt tatcataaac
      241 aattatcact cgcaatttgc tcatatgcca atgctttttc tcgatatatt aacatgggag
      301 aaaatattac tggcaacatt acgatagaac aactcaaaaa taagggggtg ataacccctt
      361 ttgcagaacc acagataggt tattttacag tatccttcca gataatcaaa aaaaatgggg
      421 atcgatatgg attgatgaaa ttacattcaa acaaaaaaat tactattgaa gaaga
//
LOCUS       DAZGJT010000364          458 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZGJT010000364 DAZGJT010000000
VERSION     DAZGJT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..458
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.372"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>458
                     /locus_tag="ACHKFK_005087"
     CDS             <1..>458
                     /locus_tag="ACHKFK_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO9055189.1"
                     /translation="APAGVELTPGYFQITAVPKLTIYDPTVQFEFWFSEAKIADAAQV
                     ETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRASNDAAGYLEL
                     FREKIGKLHLAEALWAEIDNSQLKDEMAEMQTTITETRNEITQTVSKTLE"
BASE COUNT          117 a          105 c          141 g           95 t
ORIGIN      
        1 gcgcctgccg gtgttgaact gacgccgggg tattttcaga taacggcagt cccgaaactg
       61 accatttatg acccgacggt gcagtttgag ttctggtttt cggaggcaaa aattgcagac
      121 gccgcacagg tggaaacctc tgcccgttat ctggggaccg gcagtcagtg gagtgtctcc
      181 ggtccgcaca ttaagcccgg aaaggatttc tggttttatg tgcgcagcgt caacctggtg
      241 ggtaaatctg cttttgtgga agccagtgga cgggcgagca atgatgctgc gggctatctg
      301 gaacttttcc gggaaaagat aggaaaactg catctggcag aggcgctgtg ggcagagatt
      361 gacaacagtc agctgaagga cgagatggcg gaaatgcaga ccaccatcac agaaacccgc
      421 aatgaaatca cacagacggt cagtaaaacg ctggagga
//
LOCUS       DAZGJT010000365          449 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZGJT010000365 DAZGJT010000000
VERSION     DAZGJT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.373"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>449)
                     /locus_tag="ACHKFK_005088"
     CDS             complement(<1..>449)
                     /locus_tag="ACHKFK_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO9055190.1"
                     /translation="SSINMPCTLFETITLFDDFSADDMQYGDMEEQDFLSLGLSDISA
                     KVDPYRLIKYHFPGPNSTYGAFSVPTSGTKISQSECIDILFAEMKDLAKMFSFFGQYK
                     TLIQDLIDHFRYGNGNSFHSQELNLSFHERINKYDYNSPIRVIKECI"
BASE COUNT          151 a           73 c           80 g          145 t
ORIGIN      
        1 tcgatacatt ctttgattac acgtattggg ctattataat catatttgtt tattcgttca
       61 tgaaaagata agttcaattc ctggctatgg aaactgttgc catttccata tctaaaatga
      121 tcaatcagat cctggatgag tgttttgtat tgtccaaaaa aggaaaacat ttttgccaga
      181 tctttcattt ctgcaaataa aatatcaata cattcacttt gtgatatttt tgttccagaa
      241 gttggaacac taaaagcacc ataagtagaa ttcggcccag gaaaatggta tttaatgagg
      301 cggtaaggat caacttttgc agagatgtcg cttaagccga gagagagaaa atcttgttct
      361 tccatatccc catactgcat atcatcagca gaaaaatcat cgaataaagt aatagtttcg
      421 aaaagtgtac agggcatatt aattgatga
//
LOCUS       DAZGJT010000366          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZGJT010000366 DAZGJT010000000
VERSION     DAZGJT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.374"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..>410
                     /locus_tag="ACHKFK_005089"
     CDS             79..>410
                     /locus_tag="ACHKFK_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO9055191.1"
                     /translation="MGKKTAPLLPYTNQMLSEFGERLKLARLRRRLTAKQVAERAGMS
                     PITLRSLESGSAGVTIGAYLSVMQVLGLETDLNKLAAEDDLGRQLQDSRLIKKRGLSR
                     SSGKNEEG"
BASE COUNT          120 a           77 c          103 g          110 t
ORIGIN      
        1 cgctttgcag gtgcgttcaa tccaataaat gctaatatta tggctatcat tgccataaaa
       61 agataaaata ttatcactat gggtaaaaaa acagcaccgt tactccctta tacgaatcag
      121 atgcttagtg aatttggtga gcggctgaaa cttgctcgtt tacgacgcag gcttaccgca
      181 aagcaagttg ctgaacgtgc tgggatgtcg ccaataacat tgcgctctct ggagtcagga
      241 agtgcagggg tcacgatagg ggcatatttg tctgtgatgc aggttttggg cctggaaacg
      301 gatttaaata agcttgcagc tgaagacgac ttagggcgtc agctccagga ttcccgtttg
      361 attaaaaaaa gagggctttc tcgttcatca ggtaaaaatg aagaaggtat
//
LOCUS       DAZGJT010000367          403 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZGJT010000367 DAZGJT010000000
VERSION     DAZGJT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.375"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            147..>403
                     /gene="pagP"
                     /locus_tag="ACHKFK_005090"
     CDS             147..>403
                     /gene="pagP"
                     /locus_tag="ACHKFK_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="catalyzes the transfer of palmitate to lipid A;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO9055192.1"
                     /translation="MKVSKYVAIFFFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRW"
BASE COUNT          116 a           59 c           92 g          136 t
ORIGIN      
        1 gatgcgcgga aatatttatc ttgaactagc ggaaataaat agagcagcta ttcatattat
       61 tcttcatgtt ggggctattg aggttatgtt aattgtagct ttgctatgtt agtagtagat
      121 ttttgataaa tgtttaatgg tcacaaatga aagtgagtaa atatgtcgct atatttttct
      181 ttgtttttat tcagttaatc agtgttggta aagtttttgc taacgctgat gagtggatga
      241 caacgtttag agaaaatatt gcacaaacct ggcaacagcc agaacattat gatttatata
      301 ttcctgccat cacctggcat gcacgttttg cttacgacaa agaaaaaacc gatcgctata
      361 acgagcgacc gtggggtggc ggttttggtc agtcgcgttg gga
//
LOCUS       DAZGJT010000368          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZGJT010000368 DAZGJT010000000
VERSION     DAZGJT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.376"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(158..>395)
                     /locus_tag="ACHKFK_005091"
     CDS             complement(158..>395)
                     /locus_tag="ACHKFK_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015643 - toxic substance binding
                     [Evidence IEA];
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="colicin E1 family microcin immunity protein"
                     /protein_id="HFO9055193.1"
                     /translation="LFWCISLCGMVLYPVAKWYIEDTALKFTRPDFWNSGFFTDTPGK
                     MGLLAVYTGTVFILSLPLSMIYILSVIIKRLSVR"
BASE COUNT          148 a           78 c           68 g          101 t
ORIGIN      
        1 ataatcttac agcgcaagct tccccgtcat acagacctcg tgatcgagtt aataagacta
       61 tgatagaact acaaactaac ggagtagaac ggcaactttt agataattct tgtggcataa
      121 gttctggggt atttaaataa aatataagac aggctgtcta tcttacagac agccttttta
      181 taataacaga aagaatatat atcatactta acggaagaga taatatgaaa acagtacccg
      241 tataaacagc aagcaatccc atttttccag gtgtatcagt aaaaaaaccg ctgttccaga
      301 aatcaggccg ggtaaatttc agagctgtat cttcaatata ccattttgca accggataca
      361 aaaccattcc gcagagagaa atacaccaga acagt
//
LOCUS       DAZGJT010000369          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZGJT010000369 DAZGJT010000000
VERSION     DAZGJT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.377"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>390)
                     /locus_tag="ACHKFK_005092"
     CDS             complement(<1..>390)
                     /locus_tag="ACHKFK_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO9055194.1"
                     /translation="TVRISGVLKDGTGKPVPGCTIELKARRTTETVIVTTVAQGQPGE
                     TGSYSFDVEPGWYRVTLNTEGYAPSYVGDILVKADSEPGTLNKFLMEQDEAQYYPKAL
                     AELEAVAAEILKRAEASAASAEEAKKR"
BASE COUNT           60 a          148 c           85 g           97 t
ORIGIN      
        1 cccgtttctt cgcttcctct gcactcgccg ccgacgcttc agcacgcttc aggatttccg
       61 ctgccaccgc ttccagctct gcaagcgctt tcgggtaata ctgcgcctca tcctgttcca
      121 tcagaaattt attcagcgtt cccggctcag aatccgcctt caccagaatg tcacccacat
      181 acgacggggc gtacccttcc gtgttcagcg tcacccggta ccaccccggc tccacatcaa
      241 aactgtaact gccggtttcc cccggctgcc cctgcgccac cgtggtgact atcactgtct
      301 ctgtggtgcg tcgcgctttc agctctatgg tgcatcccgg caccggcttc ccggtgccgt
      361 ccttcagcac gcccgatatt ctgactgtca
//
LOCUS       DAZGJT010000370          390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZGJT010000370 DAZGJT010000000
VERSION     DAZGJT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.378"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..229
                     /locus_tag="ACHKFK_005093"
     CDS             <1..229
                     /locus_tag="ACHKFK_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HFO9055195.1"
                     /translation="EIVNATSKDKSLKGKKLEKYLDTLVDHFIKITEHPKKGDLIFYP
                     NSPEDGKPENILKIVKEWRRSQGLPLFKDSE"
BASE COUNT          143 a           72 c           69 g          106 t
ORIGIN      
        1 ggagatagta aatgcaacta gcaaagataa atccctaaaa gggaaaaaat tagaaaaata
       61 tctcgatacc ttagtggatc atttcattaa aattactgaa catcctaaaa agggagatct
      121 cattttctat cctaattccc cagaagatgg taaacctgaa aacatcctga aaatcgtcaa
      181 agaatggcgg cgttcgcaag gattgccttt gttcaaagat tcagaatgaa ctctaaacag
      241 aatctgtcac ctgaaaatca tcacgttctg gttaagtgat gattttcgct acgagaaggt
      301 tacggtggcg attgcagtga taataagcaa tgtaaaaaga aaacataggt aaatactacc
      361 tttctctcct tccctgaaca ttcattttac
//
LOCUS       DAZGJT010000371          376 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZGJT010000371 DAZGJT010000000
VERSION     DAZGJT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.379"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..120
                     /locus_tag="ACHKFK_005094"
     CDS             <1..120
                     /locus_tag="ACHKFK_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO9055196.1"
                     /translation="LCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            117..>376
                     /locus_tag="ACHKFK_005095"
     CDS             117..>376
                     /locus_tag="ACHKFK_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HFO9055197.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYEN"
BASE COUNT          101 a           98 c          106 g           71 t
ORIGIN      
        1 ctctgttccc agatatttcg ccgcgacatt cgcgcatcgt cagaactgac acaggccgaa
       61 gcagtgaaag ctcttggatt cctgaaacag aaagccactg agcagaaggt ggcagcatga
      121 caccggacat tatcctgcag cgtaccggga tcgacgtgag agctgtcgaa cagggggatg
      181 atgcatggca caaattacgg ctcggcgtca tcaccgcttc agaagttcac aacgtgatag
      241 caaagccccg ctcaggaaag aagtggcctg acatgaaaat gtcctacttc cacaccctgc
      301 tggctgaggt ttgcaccggt gtggctccgg aagttaatgc taaggcgctg gcctggggaa
      361 aacagtacga gaacga
//
LOCUS       DAZGJT010000372          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZGJT010000372 DAZGJT010000000
VERSION     DAZGJT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.380"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            264..>374
                     /locus_tag="ACHKFK_005096"
     CDS             264..>374
                     /locus_tag="ACHKFK_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO9055198.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTT"
BASE COUNT           88 a           92 c           90 g          104 t
ORIGIN      
        1 agcgaagagc ccagtcggaa gactgggctt ttttgcgttg gtgcggaata aatgccgcat
       61 cttatccgac cttgtattat ccctccagtg cagagaaaat cggccagttt tctctgcctg
      121 cagtccgcat gccgtatccg gccttgggtt ctaacctgca tggtagattt atgcggcgga
      181 ctgcctttct cccgaagtga taaaccggac agtatcatag accggttttc ctggtaatcc
      241 gtatttgcaa ggttggtttc actatggaac atgaacttca ttatatcggt atcgacaccg
      301 ctaaagagaa actggatgtc gatgtgttgc gtcctgatgg tcgtcatcgc accaaaaaat
      361 tcgctaacac cact
//
LOCUS       DAZGJT010000373          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZGJT010000373 DAZGJT010000000
VERSION     DAZGJT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.381"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>344)
                     /locus_tag="ACHKFK_005097"
     CDS             complement(<1..>344)
                     /locus_tag="ACHKFK_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055199.1"
                     /translation="TGLKPSVVKKGENQYAVEMDNPAFSEDVATETINTLADGERITD
                     ADPMEQPAFMRDPRFRGFTGDDTEVQARLARGNAPTAEELVRSQMAEGDAGPTAQELT
                     ERPRLPAPGDIHP"
BASE COUNT           70 a          104 c           92 g           78 t
ORIGIN      
        1 ggatgaatat cgccgggagc gggaaggcgt ggacgctcag ttaactcctg tgctgtcgga
       61 ccggcatcac cttcagccat ctgggaacgc accagttctt ccgccgtcgg cgcgttgcca
      121 cgggcaagac gggcctgcac ttctgtatca tcccccgtaa aaccacggaa tcgcgggtca
      181 cgcatgaacg cgggctgttc catcggatca gcatcagtaa tacgctctcc gtcagccaga
      241 gtgttaatgg tctccgttgc cacatcttca gagaacgcag gattatccat ttcaacggca
      301 tactgattct cgcctttctt cactacggac ggtttcaggc cggt
//
LOCUS       DAZGJT010000374          339 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZGJT010000374 DAZGJT010000000
VERSION     DAZGJT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.382"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..>339)
                     /locus_tag="ACHKFK_005098"
     CDS             complement(80..>339)
                     /locus_tag="ACHKFK_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO9055200.1"
                     /translation="VVINTRSLIEHGNEIGCCSNISTNVVLNGDVSVGEETFVGSCTV
                     VNGQLKLGSKSIIGSGSVVIRNIPSNVVVAGTPTRLIRGNE"
BASE COUNT          113 a           83 c           36 g          107 t
ORIGIN      
        1 gcttttaata tcatttctct tgcaatatca acactaccat tatggttgca accaatttca
       61 gcaacgatat atatattact cattcattcc ccctaattaa tcttgttgga gtcccagcaa
      121 ctacaacatt acttggtata tttctaatta caaccgaccc agaaccaata atactctttg
      181 agcctagctt caactggcca tttacaacag tacagctacc aacaaaagtt tcttctccaa
      241 cagaaacatc accattaagt acaacattag tagagatatt gctacagcag cctatttcat
      301 taccatgttc aattaaactc ctagtattta ttacaacgg
//
LOCUS       DAZGJT010000375          338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZGJT010000375 DAZGJT010000000
VERSION     DAZGJT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.383"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>338)
                     /locus_tag="ACHKFK_005099"
     CDS             complement(<1..>338)
                     /locus_tag="ACHKFK_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO9055201.1"
                     /translation="GDSTVSLGFARIRFPGLKDFVRDAGVYNRDTFRNVVNVNHFNSS
                     AEYENAIARGHDGTAKSPLGMSIRYRYEITDELGVIASFTWARSMTNAQAFIDVKPSD
                     PSREVKNPAA"
BASE COUNT           85 a           89 c           76 g           88 t
ORIGIN      
        1 agctgccggg ttttttacct ctcgtgatgg atcggaaggc ttaacatcaa taaatgcctg
       61 cgcattagtc atggagcgcg cccatgtaaa agaggctata acacccagtt catcagttat
      121 ctcgtaacga tacctgatgc tcatccccag ggggcttttg gctgtgccgt catgcccccg
      181 ggcaatagca ttttcgtatt cagctgatga attaaaatgg tttacgttta caacattcct
      241 gaacgtatcc cggttataaa cacccgcgtc tctaacaaaa tccttcagcc cggggaagcg
      301 tatgcgtgca aacccgagag ataccgtact atcgcctg
//
LOCUS       DAZGJT010000376          331 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZGJT010000376 DAZGJT010000000
VERSION     DAZGJT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.384"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..>331
                     /locus_tag="ACHKFK_005100"
     rRNA            52..>331
                     /locus_tag="ACHKFK_005100"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           99 a           72 c           96 g           64 t
ORIGIN      
        1 caaattttcg caacacgatg atggatcgca agaaacatct tcgggttgtg aggttaagcg
       61 actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt gctaatctgc
      121 gataagcgtc ggtgaggtga tatgaaccgt tataaccggc gatttccgaa tggggaaacc
      181 cagtgtgttt cgacacacta tcattaactg aatccatagg ttaatgaggc gaaccggggg
      241 aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc cagtagcggc
      301 gagcgaacgg ggaggagccc agagcctgaa t
//
LOCUS       DAZGJT010000377          330 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZGJT010000377 DAZGJT010000000
VERSION     DAZGJT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.385"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>330
                     /locus_tag="ACHKFK_005101"
     CDS             <1..>330
                     /locus_tag="ACHKFK_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HFO9055202.1"
                     /translation="FGLSKGQNYGIIEACRNGIVTSTTALVNGQAIDHAVQLSRDEPS
                     LAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQELASQYLRFIE
                     LFGRKPTH"
BASE COUNT           77 a           72 c          100 g           81 t
ORIGIN      
        1 tttggcttaa gcaaaggtca gaactacggc attatcgagg cctgtcgcaa tgggattgtc
       61 acgtcgacga cggcgctggt gaatgggcag gctattgacc atgcggtgca gttgagccgt
      121 gatgaaccga gtctggccat agggatgcac tttgtcctta ctatgggcaa gcctctgaca
      181 gctatgccgg ggttaacccg cgatggtgtg ctgggaaaat ggatctggca gttggcagaa
      241 gaagatgctt taccgctgga agaaattact caggagctcg ccagtcagta tttgcgtttc
      301 attgagctat ttggacgcaa acctacgcat
//
LOCUS       DAZGJT010000378          324 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZGJT010000378 DAZGJT010000000
VERSION     DAZGJT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.386"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>324)
                     /locus_tag="ACHKFK_005102"
     CDS             complement(<1..>324)
                     /locus_tag="ACHKFK_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO9055203.1"
                     /translation="PDKGFDCSGLVFYAYNKILEAKLPRTANEMYHYRRATIVANNDL
                     RRGDLLFFHIHSREIADHMGVYLGDGQFIESPRTGETIRVSRLAEPFWQDHFLGARRI
                     LTEET"
BASE COUNT           79 a           93 c           78 g           74 t
ORIGIN      
        1 atcgtctctt ccgtcaaaat ccgtcgcgcg cctaaaaaat ggtcctgcca gaaaggttcg
       61 gctaaccgac ttacccgaat ggtttccccg gtgcgcggtg actcgataaa ctgaccgtct
      121 ccaagatata cgcccatatg gtcggctatc tcacgactgt ggatatggaa aaacagcaaa
      181 tctccccggc gcaggtcgtt attcgccaca atcgttgccc gacggtagtg gtacatctca
      241 ttggccgtgc gcgggagctt agcctcaagg atcttgttgt aggcataaaa aaccaacccg
      301 ctacagtcga agcctttatc aggc
//
LOCUS       DAZGJT010000379          319 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZGJT010000379 DAZGJT010000000
VERSION     DAZGJT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.387"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>319)
                     /locus_tag="ACHKFK_005103"
     CDS             complement(<1..>319)
                     /locus_tag="ACHKFK_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055204.1"
                     /translation="KGDAGPRGETGPAGPRGQKGDTGPAGPQGPAGPAGPQGVAGRDG
                     NVSTGKYAVGSYVFGVSSVYADGEGLFSSGIYENIQGSSIDLISIQTTQTGASTPNQI
                     WFLK"
BASE COUNT           72 a           91 c           75 g           81 t
ORIGIN      
        1 gtttcaaaaa ccagatttga tttggtgttg atgcgcctgt ttgtgttgtc tgtattgaaa
       61 tcagatcaat ggagctaccc tgaatgtttt catatatccc tgatgaaaat aagccttcgc
      121 cgtcagcata gacagaggac acgccaaaaa cataagaacc aaccgcatat ttcccggtgg
      181 aaacattgcc gtcccttccg gcaaccccct gtgggcctgc cgggccagct ggtccctgcg
      241 gacctgcagg tcctgtatca cctttttgac cccggggtcc ggccgggcct gtttcaccac
      301 gaggaccagc atcaccttt
//
LOCUS       DAZGJT010000380          301 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZGJT010000380 DAZGJT010000000
VERSION     DAZGJT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.388"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHKFK_005104"
     CDS             <1..159
                     /locus_tag="ACHKFK_005104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HFO9055205.1"
                     /translation="DRERINAARDKLRAETDILTSQRRGVVTPVSDIVSSLHEMSNSG
                     RLDDIPEE"
     gene            140..>301
                     /locus_tag="ACHKFK_005105"
     CDS             140..>301
                     /locus_tag="ACHKFK_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055206.1"
                     /translation="MTFRKNEPRCDEPSEMTGTEQRLFIMTKLSNPWWRLNHLYKIQN
                     EKGELVTFRM"
BASE COUNT           91 a           70 c           81 g           59 t
ORIGIN      
        1 gaccgggaaa gaatcaacgc tgccagagat aaactcagag cggaaaccga tattctgacc
       61 agccagcgtc ggggcgttgt tacgcctgtc agtgacatcg tgtcatcgct gcatgaaatg
      121 agtaattcgg ggagactgga tgacattccg gaagaatgaa ccgcgatgtg atgaaccgtc
      181 agaaatgacc gggaccgaac aacgtctgtt catcatgact aaactgagca atccctggtg
      241 gcggctcaat catctctaca agatacagaa cgaaaaaggt gagctggtca ccttcagaat
      301 g
//
LOCUS       DAZGJT010000381          295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZGJT010000381 DAZGJT010000000
VERSION     DAZGJT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.denovo.389"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            25..>295
                     /locus_tag="ACHKFK_005106"
     CDS             25..>295
                     /locus_tag="ACHKFK_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO9055207.1"
                     /translation="MSRKLHYGLSVAVLALIATGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKEKCAQVNAIERDKALAWVE"
BASE COUNT           71 a           67 c           93 g           64 t
ORIGIN      
        1 aagctgcgcg gggagagtaa gctgatgagc aggaaactcc actatggttt atcggtagcc
       61 gttctggcgc tgattgccac aggtgcttct gcgcctgaaa tcctcgacca gtttcttgac
      121 gaaaaagaag gtaaccacac cacggcatac cgtgatggtg cggggatctg gaccatctgc
      181 cgtggtgcca tcatggtgga tggcaaacct gtcgtcccgg gcatgaagtt gtcgaaggaa
      241 aaatgcgctc aggttaatgc cattgaacgt gataaggcgc tggcatgggt ggaga
//
LOCUS       DAZGJT010000382        10671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.134, whole genome shotgun sequence.
ACCESSION   DAZGJT010000382 DAZGJT010000000
VERSION     DAZGJT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.134"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(63..1775)
                     /locus_tag="ACHKFK_005107"
                     /pseudo
     CDS             complement(63..1775)
                     /locus_tag="ACHKFK_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            1747..2532
                     /gene="lafU"
                     /locus_tag="ACHKFK_005108"
     CDS             1747..2532
                     /gene="lafU"
                     /locus_tag="ACHKFK_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO9055208.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     LPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAKIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRAR"
     gene            2603..3658
                     /gene="dinB"
                     /locus_tag="ACHKFK_005109"
     CDS             2603..3658
                     /gene="dinB"
                     /locus_tag="ACHKFK_005109"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO9055209.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERAVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKSDLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            3710..4003
                     /gene="yafN"
                     /locus_tag="ACHKFK_005110"
     CDS             3710..4003
                     /gene="yafN"
                     /locus_tag="ACHKFK_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HFO9055210.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAEQYLNDMTDDDFNDFKE"
     gene            4006..4404
                     /gene="yafO"
                     /locus_tag="ACHKFK_005111"
     CDS             4006..4404
                     /gene="yafO"
                     /locus_tag="ACHKFK_005111"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HFO9055211.1"
                     /translation="MRVFKTKLIRLQLTAEELEALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDAAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            4414..4866
                     /locus_tag="ACHKFK_005112"
     CDS             4414..4866
                     /locus_tag="ACHKFK_005112"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO9055212.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFLRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAQPVGFITRIGHYIDMLFVEPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGTWFTNFYMRYKPQH"
     gene            5057..6196
                     /locus_tag="ACHKFK_005113"
     CDS             5057..6196
                     /locus_tag="ACHKFK_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO9055213.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESSAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRNSLGSIGGGNHFAELQQIDQIIDAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALRYIAEHDDALAFARINRQL
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWLVKPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVLAGLIIPVARLRPVLTLKNSGGKKG"
     gene            6193..6807
                     /gene="prfH"
                     /locus_tag="ACHKFK_005114"
     CDS             6193..6807
                     /gene="prfH"
                     /locus_tag="ACHKFK_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO9055214.1"
                     /translation="MILLQLSSAQGPEECCLAVRKALDRLIKEATRQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAALKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(6864..8321)
                     /gene="pepD"
                     /locus_tag="ACHKFK_005115"
     CDS             complement(6864..8321)
                     /gene="pepD"
                     /locus_tag="ACHKFK_005115"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO9055215.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVDTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            8582..9040
                     /gene="gpt"
                     /locus_tag="ACHKFK_005116"
     CDS             8582..9040
                     /gene="gpt"
                     /locus_tag="ACHKFK_005116"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO9055216.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQNTWIEQPWDMGVVFVPPISGR"
     gene            9132..10376
                     /gene="frsA"
                     /locus_tag="ACHKFK_005117"
     CDS             9132..10376
                     /gene="frsA"
                     /locus_tag="ACHKFK_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO9055217.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFSHGAQLPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDGLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITDWIEKRLC"
     gene            10434..>10671
                     /gene="crl"
                     /locus_tag="ACHKFK_005118"
     CDS             10434..>10671
                     /gene="crl"
                     /locus_tag="ACHKFK_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO9055218.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKA"
BASE COUNT         2582 a         2697 c         2820 g         2572 t
ORIGIN      
        1 tcacccgtag gccggataag gcgttcacgc cgcatccggc aatatgcacc gatgccggat
       61 gcctacatca acgcccccat tatcttcaac tccagctcat ccggcacttc gttatacgac
      121 agcacatgca gccctggtgc aaacaacctt gcgtagcgcg ccagcaaagg gcgcagctgc
      181 ggcggcacca gcagcaccgg atctttcccc gccgctttca tctgctcctt cacctgaggc
      241 atcgtgctct ggaactggtt gagcatattc ggatccactg gcacgctatc aagcatcact
      301 ttgccgccct gctgcgcctg attcaccacg ttggtcagca gattttccag ctcattattc
      361 agcgtataca ccgtcagctc ctgcttgcga acgaacggat gggttatgct gcgccgcagc
      421 gccaggcgca catcggccgc cagcagaata tgatctttag tcaccgcact actggcgacc
      481 aacacggtgg cgatggtgac gatatcgcgc agggaaacgc cttcagtcag tagcgcccgg
      541 tacactttca gcaactggct gtaattgagc gccgcgctca aatcttccgc cagtcgcggt
      601 gccatcgacg acaaacggtt atgcagctga gtaatatcgt catagttaaa caaatcagga
      661 atatagctgc gcacaatctt attcacatgg gtggcaatta cgctggcgct gtcgatcacc
      721 tgatacccca tattcagcgc cttcgctttc tgcgcgggct gaatccaggt gaccggcatc
      781 ccgtacgccg ggtcgttccc cagcacgccg tctatctcgc cgtaggtttc gctggagggc
      841 agggccatca gtttatctgc cggaatatcc gcttcatcag ccttaatgcc gttgatgaaa
      901 atggcgtact ggctgggctt gaggcggaag ttttcccgaa tacggatctc cggcagcagc
      961 acgccgttgc cgtcggaaat cacctgacgt acgccgcgaa tacgctgggt gagcgggttg
     1021 ccctgcgctt tatccaccag cgccaccagt ttgtagccga ggcttaaact gataggttcg
     1081 atcagcggaa tagtttccca actgacctgt tgctcgctgg tttctgtcat cgtacgggtt
     1141 agcgtttcga ggcttttctc ttccgcctcc gccgcttgcg ggcgcttgct catccgccag
     1201 ccggtaaaac caagcagggc gctgaacatc aggaacggga aatgcggcat tcccggcacc
     1261 accgccagca cgaacataat tccagtggcg gtgtagagaa ccgacgggct tgccagcaac
     1321 tgattacgca cgtcatgggc gatatcaccg ttatcgctga cgcgggtgac gataatcgcc
     1381 gccgcggtgg agagcagcag ggaagggatt tgcgccacca gcccatcgcc gatagtcatc
     1441 agcacatatt gctggaaggc ggcgtcggcg ctcaggttgt atttgaagat gccgatacag
     1501 acgccgccga tcaggttgat cgccagaatc atcatcccgg cgatggcgtc cccgcgcaca
     1561 aattttgatg ccccgtccat cgcaccgtag aaatcggcct cgctggcaac atctttacgc
     1621 cgggtctgcg cctgcgcctg attgatcaac ccggcgttaa gatcggcgtc aatcgccatc
     1681 tgcttaccgg gcatcgcgtc taaggtaaag cgggcagaaa cctcggaaat acgctcggcc
     1741 cctttggtga cgacgataaa gttgattgtc aacagcgtca gtaaatccga gcgtgaaagc
     1801 attatcgccg cgctgcacgg gcagtcgatt tttaacggtg gtgggttgtc gccgttaaat
     1861 aaaatcagcc cgtctcatct gccaaaacct gccaccgtgg cggtgccgga agagacggag
     1921 aaaaaagcgc gcgacgtcaa cgagaaaacg gcgctgttga agaagaaatc cgccaccgag
     1981 cttggcgagc tggcgaccag tatcaatacc atcgcccgtg atgcgcatat ggaagcgaat
     2041 ctggagatgg agattgttcc ccagggatta cgcgtgctga ttaaagacga ccagaaccgc
     2101 aatatgtttg aacgcggcag cgcgaagatt atgccgttct ttaaaacgct gctggtggag
     2161 cttgcgccgg tgttcgactc gctcgataac aaaattatta ttaccgggca tactgatgcg
     2221 atggcctata aaaacaatat ctacaacaac tggaaccttt cgggcgatcg cgcgctttcg
     2281 gctcgtcggg tgctggaaga ggctggaatg ccggaagata aagtgatgca ggtaagcgca
     2341 atggcggacc agatgctgct ggatgccaaa aatccgcaaa gcgcgggcaa ccggcgcatt
     2401 gagattatgg tgctgaccaa aagtgcgtcc gatacgctgt atcaatactt tggtcagcat
     2461 ggggataaag tggtgcagcc gctggtgcaa aagctggata agcagcaggt gctttcgcag
     2521 cgagcgcgtt aaatgctgaa tctttacgca tttctcaaac cctgaaatca ctgtatactt
     2581 taccagtatt gagaggtgag caatgcgtaa aatcattcat gtggatatgg actgcttttt
     2641 cgcagcggtg gagatgcgcg ataatcccgc cctgcgcgat atccctattg ctattggcgg
     2701 cagccgcgaa cgacgggggg tgatcagcac cgccaattat cccgcgcgta aatttggcgt
     2761 acgtagcgct atgccgacag ggatggcgct caaattatgc ccgcatctca ccttgcttcc
     2821 agggcgcttt gacgcctaca aagaagcctc aaatcatatc cgcgaaattt tctcgcgcta
     2881 tacctcgcgc attgaaccgt tatcactgga tgaagcctat ctcgatgtca ccgatagcgt
     2941 ccattgccac ggttctgcga ccctcatcgc ccaggaaatc cgccagacga tcttcaacga
     3001 gctgcaactg acggcgtctg cgggcgttgc gccggtcaag tttctcgcca aaatcgcctc
     3061 cgacatgaat aaacccaacg gccagtttgt gattacgccg gcagaagttc cggcatttct
     3121 gcaaacctta ccgctggcaa aaattcccgg cgtcggcaaa gtctcggcgg caaaactgga
     3181 agcgatgggg ctgcggacct gcggtgatgt gcaaaagtgt gatctggtga tgctgcttaa
     3241 acgctttggc aaatttggcc gcattttgtg ggagcgtagt cagggcattg acgaacgcgc
     3301 tgttaacagc gaacggttgc gaaaatccgt cggcgtggaa cgcacgatgg cggaagatat
     3361 tcatcactgg tctgaatgtg aagcgatcat cgagcggctt taccctgaac tggagcgtcg
     3421 tttggcaaag gtaaaacctg atttgctgat tgcccgccag ggggtgaaat taaagttcga
     3481 cgattttcag caaaccaccc aggaacacgt ctggccgcgg ctgaataaat ctgatctaat
     3541 cgccaccgcg cgtaaaacct gggatgaacg ccgcggcggg cgcggtgtgc gactggtggg
     3601 gctgcatgtg acgttgcttg acccgcaaat ggaaagacaa ctggtgctgg gattatgatg
     3661 tatactattt tgtatattct ggtgtgcatt attatgagga gatcactgta tgcatcgaat
     3721 tctcgctgaa aaatcggtca atatcactga gttacgtaaa aacccagcta aatactttat
     3781 tgatcaacct gttgcggttc tttctaataa tcgccccgca ggatatctct taagtgccag
     3841 cgcatttgaa gcattaatgg acatgcttgc agaacaagag gagaaaaagc ccataaaggc
     3901 gcgcttccgt ccgagtgctg caagattaga ggaaattaca cgccgcgctg aacaatatct
     3961 taatgatatg acggatgatg atttcaatga ctttaaggaa taaggatgcg ggtattcaaa
     4021 acaaaactta ttcgcctgca acttacagca gaggaacttg aagcgttaac ggcggatttt
     4081 atttcctata agcgtgacgg tgttttgcca gatatatttg gtcgtgatgc actctacgac
     4141 gactcgttta cctggccatt aatcaaattt gagcgagttg ctcatattca tctggcaaat
     4201 gtgaataatc catttccgcc acagttgcgc caattcagca gaacgaatga cgcagcgcat
     4261 ttggtatatt gtcagggcgc gtttgatgag caagcatggt tgctcattgc cattctgaaa
     4321 cctgaacctc ataaactggc tcgagataac aaccaaatgc ataaaattgg gaaaatggca
     4381 gaagcgtttc gcatgcgttt ttgaatttat attatgaata acatacaaat aagaaactat
     4441 cagcctggtg attttcagca actatgcgct attttccttc gagctgttac gatgacggcg
     4501 agtcagcact attctccaca gcaaattgcc gcctgggcgc agattgacga atctcgctgg
     4561 aaggagaaac tcgcgaaatc acaagtgcgg gttgcagtta ttaatgcaca accggttggt
     4621 tttattaccc gcattggaca ttatatcgat atgttatttg ttgaaccgga atacacccga
     4681 cgcggagttg ccagcgcttt gttaaaacca ttgattaagt ctgaatccga acttacggtg
     4741 gacgccagca taaccgcaaa accctttttt gaacgttatg gttttcagac agttaagcag
     4801 cagcgcgttg aatgccgggg aacgtggttt actaattttt atatgcgata taaaccgcaa
     4861 cattaaatcc agcttgcaat gaaaataacg cccgtctagt atgtgcgcgt tttcttattc
     4921 cacaaactgt aaggaggtaa atcatgacaa atcctttatc aatgactctt tgcagacctt
     4981 tccaggatta attctttttt tcttgccctg gattcgtctg ccatttcctg atttttattc
     5041 ataaatggaa tggtacatgg gcaaatatat tcgtccctta tccgatgcgg tatttaccat
     5101 cgcatccgat gacctgtgga tcgaaagttc agcgatccaa caattacaca ccacggcaaa
     5161 tttacccaac atgcagcgcg ttgtggggat gccagattta catcccggac gcggctaccc
     5221 gattggcgca gcgttcttct ctgttggtcg tttttacccg gcactggtcg gcaatgatat
     5281 cggctgcggc atggcgctat ggcaaacaga tattctcgct cgcaaatata acgccgataa
     5341 gtttgaaaag cgattatcag atctggatga cgttgctgaa gaaagctggc tggaggaaaa
     5401 cctgccgtca gcatttgcac agcatccgtg gcgcaactcg cttggctcca tcggtggcgg
     5461 taaccacttc gcagaactgc aacagattga tcaaattatc gacgcggaac tgtttgcact
     5521 ggctggtctg gatgcgcagc atctgcaact gctggttcat agcggctcga ggggattggg
     5581 gcagtctatt ttacagcggc atattgcctc gttttcgcat catggtttgc ctgaaggcag
     5641 tgacgacgcg ctacggtata ttgcggaaca tgacgatgcg ctggcgtttg cgcgtattaa
     5701 tcgccagctg atcgctttgc gcataatgca acaagttaag gccaccggaa gtccggttct
     5761 ggatgtggcg cataactttg ttagcgcgtg ccaaatcggc gatcaacagg gctggttgca
     5821 tcgtaaaggc gccacgccgg atgacaacgg tctggtgatt attcccggtt cacgcggcga
     5881 ttactcctgg ctggttaaac ctgttgcgaa cgaaaaaacg ctccactcgc tggcgcatgg
     5941 ggcggggcgt aaatgggggc gcaccgagtg taaagggcgt ctggcagcga aatacacggc
     6001 gacgcaactc tcgcggactg aacttggcag tcgggtaatt tgtcgcgata aacaactcat
     6061 ctttgaagaa gcgccacaag cctataaatc ggctgaaagc gtggtgcaat gtctggtgct
     6121 ggctgggtta attattcctg tcgcgcgact gcgcccggtg ctgacgctca aaaacagtgg
     6181 agggaaaaaa ggatgatctt gctacaactc tcctctgctc aggggccgga agaatgttgt
     6241 ctcgcagtga gaaaagcact ggacaggctg attaaagaag ctacccgaca ggacgtcgcg
     6301 gtaacggtgc tggaaacaga aacgggtcgc tactctgaca cactgcgttc ggcgctgatt
     6361 tctctggatg gcgataacgc atgggctcta agcgaaagct ggtgcggcac tattcagtgg
     6421 atttgtccga gtccgtatcg gcctcatcat gggcgcaaaa actggtttct gggcattggg
     6481 cgttttaccg ctgatgagca ggaacaatcg gatgcaatcc gttatgagac gctgcgttcg
     6541 tcggggccgg gcggtcaaca tgtcaataaa accgactcgg cggtacgcgc cacgcatctg
     6601 gcatccggta ttagcgtgaa ggttcagtca gagcgcagtc agcatgctaa caaacggctg
     6661 gcgcgattac tgattgcctg gaagctggaa caacagcaac aggaaaatag cgcggcgctg
     6721 aaatcgcagc ggcgaatgtt ccatcaccag attgaacgtg gcaacccgcg acgaacgttt
     6781 acagggatgg cttttatcga agggtaatga aggatgaaac tgccggaagg cgattaaacg
     6841 ccatccggca gcaaatcaaa taattacttc gccggaattt ctttcagcag ttcagtcagc
     6901 agtgtccagt aatggccaac gctttcgata tgaacttgct catccgggga gtgtggaccg
     6961 gtgatggttg gcccgataga caccatgtcc atttccggat acggtttttt gaacaggcca
     7021 cattcgaggc ccgcgtggat aatctggatg ttcggcgttt tgttgaacag acgctgatag
     7081 gtttcacgta ccagatgcat taccggagag ttagcgtccg gctgccagcc aggatatgcg
     7141 ccttttgctt cggttttcgc acctgccagc ttacccagcg aatccagcat gctcactacg
     7201 tagtctttac cgctgtcgat cagagaacgg atcaggcagt gaatttctac gttattgtcg
     7261 gtcatggtca ccacaccgac gttcagggag gtttcgacca caccttttgc cacgtcggag
     7321 ttgcggatca caccgttcgg ggtggcgttc agcagacgaa taaaggtatc gcgagatttc
     7381 gcaatcaggg cagctttatc gttcgcaaca gagtccagca gcaatgccag atttttctct
     7441 ttctctgcca gttcgttttt taggatctcc tgataggtat ccaccagaga tttcagggcg
     7501 tcgactttat cagctgcgac agcaatggtc gcaaaggctt cacgcgggat ggcgttacgc
     7561 agtgtgccgc cgttgaaatc aataagacgc aggtccagtt cttctgcatg acccgccagg
     7621 aagcgcacca acagtttgtt ggcattaccc aggccaacgt ggatttcacc gccggagtga
     7681 ccgcctttca gaccttttaa ggttaacttg aaggtttcaa aaccagctgg aaccgcttca
     7741 cgatctaaat gcaggttgga ggtgaagtcg atacctcccg cacaacccat gtagatttca
     7801 ccttcttctt cggagtcggt gttaatcaga atatcagcct gcaaccagtt gctctgtaag
     7861 ccaaaagcac cgtccatacc ggcttcttcg gtcatggtca gcagcacttc cagcgggccg
     7921 tgaaccacgt tttcgtcagc cagaaccgcc agtgcagagg ccataccaat gccgttatcc
     7981 gcacccagcg tggtgccgcg tgctttaacc cattcgccat caatataagg ctggatagga
     8041 tctttagtga agtcatgcac ggtgtcgtta tttttttgcg gcaccatatc gaggtgggcc
     8101 tgcaagacga ccggtttacg attttccata cctgcggtag caggtttacg aatcaggata
     8161 ttacctacct gatcgcgttc gacatggaaa cctttctctt ttgcccaacc aacaatgtat
     8221 tcagcgagtt gctcttcatg ataggacggg tgaggaatag aacagatttt ggcaaaaata
     8281 tcccacagcg gctgtggaga taattgagac agttcagaca cgttaagtct ccttgtcgat
     8341 cacccgcaaa acagtattgc aggtcacagg gttagcagaa aatgttgtca acacaagaca
     8401 ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag gcagtgcgta
     8461 aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc
     8521 gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca
     8581 catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc
     8641 aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct
     8701 ggtaccgggt gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat
     8761 ttccagctac gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga
     8821 tggcgaaggc ttcatcgtta ttgatgacct ggtagatacc ggtggtactg cggttgcgat
     8881 tcgtgaaatg tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ccggtcgtcc
     8941 gctggttgat gattatgttg ttgatattcc gcaaaatacc tggatcgaac agccgtggga
     9001 tatgggcgtc gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcact
     9061 gccgggcgtt gttcttttta acttcaggcg ggttacaata gtttccagta agtattctgg
     9121 aggctgcatc catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac
     9181 atcctgaaac ctcgacgcta gtccgccgct ttagtcacgg cgcacaactg cctgtgcagt
     9241 cggcccttga tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga
     9301 tctggcgcgg cattgaccca cgtgaaatcc tcgatgtcca ggcgcgtatt gtgatgagcg
     9361 atgccgaacg taccgacgat gatttatacg atacggtgat tggctaccgc ggcggcaact
     9421 ggatttatga gtgggctacc caggcgatgg tgtggcaaca aaaagcctgt gcggaagaag
     9481 atccgcaact cagcggtcga cactggctgc atgcggctac gttgtacaac attgctgcct
     9541 atcctcatct gaaaggagat ggcctggccg agcaagcgca ggctttgtca aaccgcgcct
     9601 atgaagaggc cgctcagcgt ctgccgggca cgatgcggca gatggagttt accgtacccg
     9661 gcggtgcgcc catcaccggc tttttgcata tgccgaaagg cgatggcccg ttcccgacag
     9721 tattaatgtg tggtggtctg gatgcgatgc agacggacta ttacagcctg tatgaacgtt
     9781 attttgcgcc gcgcggcatt gcgatgctga ctattgatat gccgtcggtg ggcttttctt
     9841 caaaatggaa gctcacccag gactccagcc tgttgcatca gcacgtctta aaggcgctgc
     9901 ctaacgtacc gtgggtagat cacactcgcg tcgcggcctt tggtttccgt ttcggcgcta
     9961 acgttgccgt gcgtctggca taccttgaat cgccgcgtct gaaagcggtt gcctgtcttg
    10021 gtccggtagt tcataccctg ttgagtgatt ttaagtgcca gcaacaggtg ccggaaatgt
    10081 atcttgacgt tctggcgagc cgtttgggga tgcatgatgc ttccgacgaa gcgttgcgcg
    10141 tggagctgaa tcgctattca ttaaaagtgc aaggattgct gggacgtcgc tgcccaacgc
    10201 caatgttatc aggctactgg aagaacgatc cgttcagccc ggaagaggac tcacgcttaa
    10261 tcacctcatc atctgctgac ggtaaattgt tagagatccc atttaacccg gtgtatcgga
    10321 attttgacaa aggccttcag gaaatcacag actggatcga aaaacgtttg tgttaaaaat
    10381 ttgctaaatt ttaccaattt ggtaaaacag ttgcatcaca acaggagatt gcaatgacgt
    10441 taccgagtgg acacccgaag agcagattga tcaaaaaatt taccgcacta ggcccgtata
    10501 ttcgtgaagg taagtgcgaa gataatcgat tctttttcga ttgtctggct gtatgcgtca
    10561 acgtgaaacc ggcaccggaa gtgcgtgagt tctggggctg gtggatggaa ctggaagcgc
    10621 aggaatcccg ttttacctac agttaccagt ttggtctgtt cgataaagca g
//
LOCUS       DAZGJT010000383        10375 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.137, whole genome shotgun sequence.
ACCESSION   DAZGJT010000383 DAZGJT010000000
VERSION     DAZGJT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10375
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.137"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(38..643)
                     /locus_tag="ACHKFK_005119"
                     /pseudo
     CDS             complement(38..643)
                     /locus_tag="ACHKFK_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(862..1839)
                     /gene="epmA"
                     /locus_tag="ACHKFK_005120"
     CDS             complement(862..1839)
                     /gene="epmA"
                     /locus_tag="ACHKFK_005120"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO9055219.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2164..3972
                     /gene="frdA"
                     /locus_tag="ACHKFK_005121"
     CDS             2164..3972
                     /gene="frdA"
                     /locus_tag="ACHKFK_005121"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO9055220.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGMAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3965..4699
                     /gene="frdB"
                     /locus_tag="ACHKFK_005122"
     CDS             3965..4699
                     /gene="frdB"
                     /locus_tag="ACHKFK_005122"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO9055221.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4710..5105
                     /gene="frdC"
                     /locus_tag="ACHKFK_005123"
     CDS             4710..5105
                     /gene="frdC"
                     /locus_tag="ACHKFK_005123"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO9055222.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIMIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5116..5475
                     /gene="frdD"
                     /locus_tag="ACHKFK_005124"
     CDS             5116..5475
                     /gene="frdD"
                     /locus_tag="ACHKFK_005124"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO9055223.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5538..6671
                     /locus_tag="ACHKFK_005125"
     CDS             5538..6671
                     /locus_tag="ACHKFK_005125"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO9055224.1"
                     /translation="MLKTTLCTLLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMKTRVFQPLKLNHTWINVPSAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLKPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDIIINGSDNKIALAARPVKPITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            6760..7293
                     /gene="blc"
                     /locus_tag="ACHKFK_005126"
     CDS             6760..7293
                     /gene="blc"
                     /locus_tag="ACHKFK_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO9055225.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7290..7607)
                     /gene="sugE"
                     /locus_tag="ACHKFK_005127"
     CDS             complement(7290..7607)
                     /gene="sugE"
                     /locus_tag="ACHKFK_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO9055226.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7783..7929)
                     /gene="ecnB"
                     /locus_tag="ACHKFK_005128"
     CDS             complement(7783..7929)
                     /gene="ecnB"
                     /locus_tag="ACHKFK_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO9055227.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(8040..8165)
                     /gene="ecnA"
                     /locus_tag="ACHKFK_005129"
     CDS             complement(8040..8165)
                     /gene="ecnA"
                     /locus_tag="ACHKFK_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO9055228.1"
                     /translation="MMKRLIVLVLLASALLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8217..8783)
                     /gene="efp"
                     /locus_tag="ACHKFK_005130"
     CDS             complement(8217..8783)
                     /gene="efp"
                     /locus_tag="ACHKFK_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO9055229.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8825..9853
                     /gene="epmB"
                     /locus_tag="ACHKFK_005131"
     CDS             8825..9853
                     /gene="epmB"
                     /locus_tag="ACHKFK_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO9055230.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVAPGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            10088..>10375
                     /gene="yjeJ"
                     /locus_tag="ACHKFK_005132"
     CDS             10088..>10375
                     /gene="yjeJ"
                     /locus_tag="ACHKFK_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO9055231.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSAMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADI"
BASE COUNT         2693 a         2650 c         2597 g         2435 t
ORIGIN      
        1 tgaacataaa acactatcaa taagttggag tcattacccc tgggttcggt gatgcaagga
       61 aattaatatc ctgcgcgaaa tgcccaccat ttaataacaa aatagtaatg cttactaaca
      121 gcaatactaa attcagacac attactgcaa taccgcccac cgcagtaatg cgggcaattt
      181 tattaatccc cttagaagca acgacggtga ccagaatcat ccatgccact gccagcagac
      241 caaccacctg cgtaggctcc agtccggcaa tacgccagtg ctgggtcatg tcgctaccat
      301 agaggaatgt tgagaacggt acccaaactt tcgcggaggt actcaccatc cagatgatat
      361 aagaggaaaa ccacataaac gtaccaatga aggcaaaacg tggtccgaca ctattattca
      421 tccaggaata gataccgcct tcttctttgc gataagcagc tcccatttca gccatcatta
      481 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
      541 ccattaagta ataagccgat gggctattgg caaatccaaa tacagaagta aagatcatca
      601 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      661 cagcgcgtgt ataaaacgcg cccactgaac tgcactgccc aacattgagc gggcagcgta
      721 aaaaccaata cagaattgat acctggatga tatcaaatat ccagtcttaa attgtggcct
      781 ttagcggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgcggt
      841 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      901 tgtctccgcg cccagcgcca acatcaccag acgatcaacg cctaatgcca cgccggaaca
      961 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
     1021 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
     1081 cgtcaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
     1141 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
     1201 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
     1261 ttgtagaagc gtgtcgcggt cttcttcagt atcggcaacg ttgctcaaat ccagtttcgc
     1321 tgccacttcc cgcagttgcg ttttgtcggc agagagtggg tcaatttcca gataacgtaa
     1381 gaaagcttgt tgataagaaa ggctttctgc tgccggacag tccagcacct gttgcaagag
     1441 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1501 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1561 gaataccggc ccacaaccgg caaccagcag gcgcttcata tggtattccg ggctggtcat
     1621 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1681 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1741 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcgcgtttta ataagttagg
     1801 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1861 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1921 aacggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1981 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     2041 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     2101 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     2161 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     2221 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     2281 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     2341 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     2401 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     2461 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2521 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2581 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2641 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2701 acgctggtgc agatccgtgc taacgcggtc gttatggcta ccggcggtgc gggtcgcgtt
     2761 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2821 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg cctaccaggt
     2881 tccggtatcc tgatgaccga aggctgccgc ggtgaaggtg gtattctggt caacaaaaat
     2941 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     3001 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     3061 cgtaaaggca acaccatctc cacgccacgt ggcgatgtgg tttacctcga cctgcgtcac
     3121 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     3181 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     3241 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     3301 gaatgttcct ctgttggtct gcacggtgcg aaccgtctgg gctccaactc gctggcggaa
     3361 ctggtggtct tcggtcgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     3421 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3481 ctggttaacc aggatggcgg cgaaaactgg gctaagatcc gcgacgaaat gggcatggcc
     3541 atggaagaag gctgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3601 ctggcagagc tgcaggaacg cttcaagcgc gtgcgcatca ccgacacttc cagcgtgttc
     3661 aacaccgacc tgctctacac cattgagctg ggccacggtc tgaacgttgc tgaatgtatg
     3721 gcgcactccg caatggcacg taaagagtcc cgcggcgcgc accagcgtct ggacgaaggt
     3781 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3841 ggcacgactc gcctggagta cagcgacgtg aagattacta cgctgccgcc agctaaacgt
     3901 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3961 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     4021 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     4081 tgcgctgggc tacatcaaag acaacctggc accggacctg agctaccgct ggtcctgccg
     4141 tatggcgatt tgtggctcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     4201 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     4261 tgaacgcgat ctggtggtcg atatgactca cttcatcgaa agtctggaag cgatcaaacc
     4321 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     4381 gatggcgaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     4441 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4501 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4561 gaacggcgta tggagctgta ctttcgtggg ctactgctcc gaagtctgcc cgaaacacgt
     4621 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4681 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4741 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4801 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4861 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcatga
     4921 tcattaacct gatcactctg gcggcagccc tgctgcacac caaaacctgg tttgagctgg
     4981 caccaaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     5041 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     5101 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     5161 ggcctcttcg gggccggtgg tatgtggagc gccatcattg cgccggtgat gatcctgctg
     5221 gttggtattc tgctgccgct agggctgttt ccgggtgatg cactgagcta cgagcgcgtt
     5281 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     5341 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat ccacgtacct
     5401 gcgggtaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     5461 gtcgttacaa tctaacgcat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5521 tatggaaacc agaccttatg ctcaaaacga cactctgcac cttattaatt accgcctctt
     5581 gctccacatt tgccgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5641 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5701 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5761 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5821 ctcgagggga aatcaagtta agcgatccca caacaaaata ctggcctgaa cttaccgcta
     5881 aacagtggaa tgggatcaca ctattacatc tcgcaaccta caccgctggc ggcctgccat
     5941 tgcaggtgcc ggatgaggtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     6001 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     6061 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgaaa actcgtgtct
     6121 tccagccact caaactcaat catacgtgga ttaatgtacc gtccgcagaa gaaaagaact
     6181 acgcctgggg atatcgcgaa ggtaaggcag tacatgtttc gccaggggcg ttagatgctg
     6241 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     6301 aaccccttga tatcaatgag aaaacacttc aacaagggat acaactggca caatctcgct
     6361 actggcaaac cggcgatatg tatcagggtc tgggctggga aatgctggac tggccggtaa
     6421 atcctgacat catcattaac ggcagtgaca ataaaattgc actggcagca cgccccgtaa
     6481 aaccgattac gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6541 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6601 tggctaacaa aaactacccc aatccagcga gagtcgccgc cgcctggcag attctcaacg
     6661 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctccgct tttccttgct
     6721 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6781 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt cccctacgcc gccgcgtggc
     6841 gtgaccgtag tgaataattt cgacgctaaa cgttatctcg gtacctggta tgagattgcc
     6901 cgttttgatc accgctttga acgtggactg gaaaaagtca ccgcaacata cagcctgcgt
     6961 gatgacggcg gcctgaatgt cattaataaa ggctataacc ctgacagaga aatgtggcag
     7021 cagagtgaag ggaaagcgta ctttaccggc gcaccaactc gcgctgcgct gaaagtgtca
     7081 ttctttggtc ctttctatgg cggttataac gttattgcac tcgatcggga ataccgccat
     7141 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     7201 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     7261 aagtttattt gggtacagca gcccggtagt taatgagtgc tgagtttcag accaataatc
     7321 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     7381 acaatgccgg tgatggccgc gccgactgcg ccgatacccg tccacacggc ataagccgtc
     7441 cctactggta acgatttcat cgcccaggca agtagcgcca tactgacaat catcgccgtc
     7501 acggtaataa cacttggcgt caaacgacta aagccgtgag tatatttcag gccaacggcc
     7561 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccaga
     7621 aaattggggc cgtccccggt gaaaagacgc gtttgcaggt cgtcctgcaa agctgatggt
     7681 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctatgt aactatcgca
     7741 gaaaaaacaa acggcacgac acagctgtcg taccgttatt gcttattgct gcgctttcgt
     7801 tgctgcgcca gaaatcgcgt taccgccatc agaaatgtct tcaccaacgc cacgcgtggt
     7861 gttgcaggca gttaatactg ttgaaagcac cagaacagaa aagatcgctg caattgtctt
     7921 cttcaccata acgtcttcct tttaggtaat gttatttatg tttgcctata gcaaattaat
     7981 aatagacaac atcgccaaaa atgatggaaa tctgatgatt tttcggagga gaagaaaaat
     8041 tagctggcag cgcgggagat ggagttgccg agatgtttga tatcttcgcc gaaaccgcga
     8101 gcggtgttac agcccgtgag cagcgcgctg gcaagcaaaa caagaacgat aaggcgtttc
     8161 atcatctctg acctgaaaaa gtgatggtgc agcctgcagg ccgcaccaca accgcattac
     8221 ttcacgcgag agacgtattc accagagcgg gtatccactt tgatgacttc gccgatctgc
     8281 acgaacagcg gaactttaac cacagcgcca gtagacaggg tagccggttt gccgccagta
     8341 cctgcggtgt cacctttcag gcccggatcg gtatcaacga tttccagttc aacgaagttc
     8401 ggcggagtaa cggagatcgg ctgaccattc cacagagtta cgatacactc tgcctgatcc
     8461 agcaaccatt tagcgttgtc gccaattgct tttgcgtcag cagacagctg ctcgaaagtt
     8521 tcgttgttca tgaagtgcca gaactcaccg tcgttgtaca ggtaagtcag gttcatatcg
     8581 acaacatcag cgccttcagc agaatcagta gatttgaagg ttttttctac gcgagtaccg
     8641 gtcagcagac gacgcagttt aacgcgagca aatgcctggc ctttacccgg ttttacgaat
     8701 tcactcgctt caaccgcgta aggttcgccg tctaacatga ttttaagacc agcacgaaaa
     8761 tcgttgctat agtacgttgc cataaggccc tctgaaattt gttaattggt agctaagcca
     8821 caaaatggcg catattgtaa ccctaaatac cccatccaga gaagattggt taacgcaact
     8881 tgccgatgtt gtgaccgatc ctgatgaact tctgcgtctt ttgaatatag acgcggacga
     8941 aaaactgttg gccggacgca gcgccaaaaa gctgtttgcc ctgcgtgtgc cccgctcatt
     9001 tatcgatcgc atggagaaag gcaatccgga cgatcctctt ttgcgtcagg tacttacctc
     9061 gcaagatgag tttgtcgtcg cgcccggatt ctccaccgac ccgctggaag aacagcatag
     9121 cgtagtgcct ggtttgttgc ataaatacca caaccgggcg cttttgctgg tcaaaggcgg
     9181 ctgcgcggta aattgccgct attgcttccg tcgccacttc ccctatgccg aaaatcaggg
     9241 caacaagcgt aactggcaga ctgcacttga gtatgttgct gcgcatccgg aactggacga
     9301 gatgattttc tccggcggcg atccgctgat ggcgaaagat cacgagctgg actggttgct
     9361 cacacaactg gaagccatcc cgcatatcaa acgtctgcgg attcacagcc gtctgccgat
     9421 tgtgatcccg gcacgcatca cagatgcgct ggttgaacgc ttttctcatt ctacgctgca
     9481 aatcttgctg gtgaatcaca tcaaccatgc caatgaggta gatgaaacat tccgtcaggc
     9541 gatggctaag ttgcgccggg tgggcgttac tttgctgaac cagagcgttc tgttacgtgg
     9601 tgtgaacgat aacgcgcaaa cgctggcaaa cctgagtaat gcgttgttcg atgcgggcgt
     9661 gatgccgtat tacctgcatg tgctcgataa agtacagggc gcggcgcatt ttatggtgag
     9721 tgatgacgaa gcacggcaga ttatgcgtga gttgctgaca ttggtgtcgg gatatctggt
     9781 gcctaaactg gcgcgagaaa ttggcggcga acccagcaaa acgccgctgg atctccagct
     9841 acgccagcag taactgatta aacagggaag tttaatcttt gcccgttgca taacctttaa
     9901 ttattcatat acattgctgt tgttactctt tatataacca acaccaataa ttaacctttc
     9961 ttttctgttt atatagagtg agcacggcta tttatgttat tttaacaact attactacca
    10021 agcccattta tctggcttat aaactaaaat taattattta attgagaaat ggttagggag
    10081 aacctacatg gccataagca tcaagggtgt taataccggt gttattcgca agagtaataa
    10141 tttcattgca ctggcgctga aaattaaaga acctcgcaat aaagaatcgt tgttctttat
    10201 gtctgccatg gaattgcgcg accttctgat tgcgctggaa agtcgtctgc atcaaaagca
    10261 taaactggat gctgctgcac gccttcagta tgaacaagct cgcgataaag ttattaagaa
    10321 aatggcagaa aatatcccag aaattctggt tgatgagctt aaaaatgccg atatt
//
LOCUS       DAZGJT010000384         9620 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.141, whole genome shotgun sequence.
ACCESSION   DAZGJT010000384 DAZGJT010000000
VERSION     DAZGJT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9620
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.141"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..2423
                     /locus_tag="ACHKFK_005133"
                     /pseudo
     CDS             30..2423
                     /locus_tag="ACHKFK_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element Tn3 family transposase"
     gene            2646..3263
                     /locus_tag="ACHKFK_005134"
                     /pseudo
     CDS             2646..3263
                     /locus_tag="ACHKFK_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611787.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3861..4103
                     /locus_tag="ACHKFK_005135"
     CDS             3861..4103
                     /locus_tag="ACHKFK_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HFO9055232.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
     gene            complement(4135..4785)
                     /gene="tetR(A)"
                     /locus_tag="ACHKFK_005136"
     CDS             complement(4135..4785)
                     /gene="tetR(A)"
                     /locus_tag="ACHKFK_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HFO9055233.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPSADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            4891..6090
                     /gene="tet(A)"
                     /locus_tag="ACHKFK_005137"
     CDS             4891..6090
                     /gene="tet(A)"
                     /locus_tag="ACHKFK_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008493 - tetracycline transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015904 - tetracycline transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HFO9055234.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(6122..7006)
                     /locus_tag="ACHKFK_005138"
     CDS             complement(6122..7006)
                     /locus_tag="ACHKFK_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFO9055235.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(7144..7536)
                     /locus_tag="ACHKFK_005139"
     CDS             complement(7144..7536)
                     /locus_tag="ACHKFK_005139"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HFO9055236.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            7540..9291
                     /locus_tag="ACHKFK_005140"
                     /pseudo
     CDS             7540..9291
                     /locus_tag="ACHKFK_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
     gene            9328..9582
                     /locus_tag="ACHKFK_005141"
     CDS             9328..9582
                     /locus_tag="ACHKFK_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO9055237.1"
                     /translation="MDEERVFSLSYEQLTRIAEKNIRECGLDSQSARCLNELRASALL
                     WLWYELAIHGAPQNNHAQARERIDTDYQRLKKLIWSEGDS"
BASE COUNT         2064 a         2820 c         2822 g         1914 t
ORIGIN      
        1 ctaaatgccc gtcaggcagg gaggccgata tgcccgttga ctttctgacc actgagcaga
       61 ctgaaagcta tggcagattc accggtgaac cggatgagct tcagctggca cgatattttc
      121 accttgatga agcagacaag gaatttatcg gaaaaagcag aggtgatcac aaccgtctgg
      181 gcattgccct gcaaattgga tgtgtccgtt ttctgggcac cttcctcacc gatatgaatc
      241 atattccttc cggcgtccgg cattttaccg ccagacagct cgggattcgt gatatcaccg
      301 ttcttgcaga atacggtcag agggaaaata cccgccgtga gcatgcagcg ctgatacgtc
      361 agcactatca gtatcgtgaa tttgcctggc cctggacatt tcgccttacc cgtcttttat
      421 atacccggag ctggataagc aacgaacgtc ctggcctgct tttcgatctg gcgacagggt
      481 ggcttatgca acatcgtatt attctccccg gagccactac gctgacccgg ttgatttcag
      541 aggtaaggga aaaggcgacg ttgcgcctgt ggaacaaact ggcactgata ccgtcagccg
      601 aacagcgttc acagctggag atgctgctgg ggccaactga ttgcagccgc ctgtctttac
      661 tggaatcact gaaaaagggc cctgtgacca tcagtggtcc ggcgtttaat gaagcaattg
      721 aacgctggaa aactctgaac gattttggcc tgcatgctga aaacctgagt acactcccgg
      781 ctgtgcgcct gaaaaatctc gcacgttatg ctggtatgac ttcggtgttc aatattgcca
      841 ggatgtcacc gcagaaaagg atggcggttc tggttgcctt tgtccttgca tgggaaacgc
      901 tggcgctgga tgatgcattg gacgttctgg acgccatgct ggccgttatc atccgtgacg
      961 ccagaaagat tgggcagaaa aaacggctcc gctcgctgaa ggatctggat aaatctgcat
     1021 tggcgctcgc cagcgcatgt tcgtacctgc tgaaagaaga aacaccggac gaatcgattc
     1081 gtgctgaggt gttcagctac atcccaaggc aaaagctggc tgaaatcatc acgcttgtcc
     1141 gtgaaattgc ccggccctca gacgataatt ttcatgaaga aatggtggag cagtacgggc
     1201 gcgttcgtcg tttcctgccc catctgctga ataccgttaa attttcatcc gcacctgccg
     1261 gggttaccac tctgaatgcc tgtgactacc tcagccggga gttcagctca cggcggcagt
     1321 tttttgacga cgcaccaacg gaaattatca gtcggtcatg gaaacggctg gtgattaaca
     1381 aggaaaaaca tatcacccgc aggggataca cgctctgctt tctcagtaaa ctgcaggata
     1441 gtctgaggcg gagggatgtc tacgttaccg gcagtaaccg gtggggagat cctcgtgcaa
     1501 gattactaca gggtgctgac tggcaggcaa accggattaa ggtttatcgt tctttggggc
     1561 acccgacaga cccgcaggaa gcaataaaat ctctgggtca tcagcttgat agtcgttaca
     1621 gacaggttgc tgcacgtctt tgcgaaaatg aggctgtcga actcgatgtt tctggcccga
     1681 agccccggtt gacaatttct cccctcgcca gtcttgatga gccggacagt ctgaaacgac
     1741 tgagcaaaat gatcagtgat ctactccctc cggtggattt aacggagttg ctgctcgaaa
     1801 ttaacgccca taccggattt gctgatgagt ttttccatgc tagtgaagcc agtgccagag
     1861 ttgatgatct gcccgtcagc atcagcgccg tgctgatggc tgaagcctgc aatatcggtc
     1921 tggaaccact gatcagatca aatgttcctg cactgacccg acaccggctg aactggacaa
     1981 aagcgaacta tctgcgggct gaaactatca ccagcgctaa tgccagactg gttgattttc
     2041 aggcaacgct gccactggca cagatatggg gtggaggaga agtggcatct gcagatggaa
     2101 tgcgctttgt tacgccagtc agaacaatca atgccggacc gaaccgcaaa tactttggta
     2161 ataacagagg gatcacctgg tacaactttg tgtccgatca gtattccggc tttcatggca
     2221 tcgttatacc ggggacgctg agggactcta tctttgtgct ggaaggtctt ctggaacagg
     2281 agaccgggct gaatccaacc gaaattatga ccgatacagc aggtgccagc gaacttgtct
     2341 ttggcctttt ctggctgctg ggataccagt tttctccacg cctggctgag gggtttgaag
     2401 cccaatggag cggaaacgta cgctaagaag ctaactatat gattttaagt caataaagtc
     2461 gtggagttgt taggcaggtt tacgttgatt tgattaccga gtttattaac actgaaagtc
     2521 cctgaaaccg catggctact ggcattgacg ggaagagtaa acaaaaccct cgttttcttt
     2581 actcacctct tctgctcaaa aaatgaactt agcgtacgat ctcgtacggt tgggcctcaa
     2641 accccgctga tgccggtgct tcggttttct ggcgaatgga ccatgatgcc gactatggcg
     2701 tgctgaatga tattgccaga gggcaatcag atccccgaaa aatagtcctt cagtgggacg
     2761 aaatgatccg gaccgctggc tccctgaagc tgggcaaagt acaggtttca gtgctggtcc
     2821 gttcattgct gaaaagtgaa cgtccttccg gactgactca ggcaatcatt gaagtggggc
     2881 gcatcaacaa aacgctgtat ctgcttaatt atattgatga tgaagattac cgccggcgca
     2941 ttctgaccca gcttaatcgg ggagaaagtc gccatgccgt tgccagagcc atctgtcacg
     3001 gtcaaaaagg tgagataaga aaacgatata ccgacggtca ggaagatcaa ctgggcacac
     3061 tggggctggt cactaacgcc gtcgtgttat ggaacactat ttatatgcag gcagccctgg
     3121 atcatctccg ggcgcagggt gaaacactga atgatgaaga tatcgcacgc ctctccccgc
     3181 tttgccacgg acatatcaat atgctcggcc attattcctt cacgctggca gaactggtga
     3241 ccaaaggaca tctgagacca ttaaaagagg cgtcagaggc agaaaacgtt gcttaacgtg
     3301 agttttcgtt ccactgagcg tcagacccca aatattggtt taaaagcagg ttaaaagaca
     3361 ggttagcggt ggccgaaaaa cgggcggaaa cccttgcaaa tgctggattt tctgcctgtg
     3421 gacagcccct caaatgtcaa taggtgcgcc cctcatctgt cagcactctg cccctcaagt
     3481 gtcaaggatc gcgcccctca tctgtcagtg agggccaagt tttccgcgag gtatccacaa
     3541 cgccggcggc cgcggtgtct cgcacacggc ttcgacggcg tttctggcgc gtttgcaggg
     3601 ccatagacgg ccgccagccc agcggcgagg gcaaccagcc cggtgagcgt cggaaaggcg
     3661 ctggaagccc cgtagcgacg cggagagggg cgagacaagc caagggcgca ggctcgatgc
     3721 gcagcacgac atagccggtt ctcgcaagga cgagaatttc cctgcggtgc ccctcaagtg
     3781 tcaatgaaag tttccaacgc gagccattcg cgagagcctt gagtccacgc tagatctatc
     3841 tcatctgcgc aaggcagaac gtgaagacgg ccgccctgga cctcgcccgc gagcgccagg
     3901 cgcacgaggc cggcgcgcgg acccgcgcca cggcccacga gcggacgccg cagcaggagc
     3961 gccagaaggc cgccagagag gccgagcgcg gccgtgaggc ttggacgcta gggcagggca
     4021 tgaaaaagcc cgtagcgggc tgctacgggc gtctgacgcg gtggaaaggg ggaggggatg
     4081 ttgtctacat ggctctgctg tagtgagtgg gttgcgctcc ggcagcggtc ctgatcaatc
     4141 gtcacccttt ctcggtcctt caacgttcct gacaacgagc ctccttttcg ccaatccatc
     4201 gacaatcacc gcgagtccct gctcgaacgc tgcgtccgga ccggcttcgt cgaaggcgtc
     4261 tatcgcggcc cgcaacagcg gcgagagcgg agcctgttca acggtgccgc cgcgctcgcc
     4321 ggcatcgctg tcgccggcct gctcctcaag cacggcccca acagtgaagt agctgattgt
     4381 catcagcgca ttgacggcgt ccccggccga aaaacccgcc tcgcagagga agcgaagctg
     4441 cgcgtcggcc gtttccatct gcggtgcgcc cggtcgcgtg ccggcatgga tgcgcgcgcc
     4501 atcgcggtag gcgagcagcg cctgcctgaa gctgcgggca ttcccgatca gaaatgagcg
     4561 ccagtcgtcg tcggcactcg gcaccgaatg cgtatgattc tccgccagca tggcttcggc
     4621 cagtgcgtcg agcagcgccc gcttgttcct gaagtgccag taaagcgccg gctgctgaac
     4681 ccccaaccgt tccgccagtt tgcgtgtcgt cagaccgtct acgccgacct cgttcaacag
     4741 gtccagggcg gcacggatca ctgtattcgg ctgcaacttt gtcatgcttg acactttatc
     4801 actgataaac ataatatgtc caccaactta tcagtgataa agaatccgcg cgttcaatcg
     4861 gaccagcgga ggctggtccg gaggccagac gtgaaaccca acagacccct gatcgtaatt
     4921 ctgagcactg tcgcgctcga cgctgtcggc atcggcctga ttatgccggt gctgccgggc
     4981 ctcctgcgcg atctggttca ctcgaacgac gtcaccgccc actatggcat tctgctggcg
     5041 ctgtatgcgt tgatgcaatt tgcctgcgca cctgtgctgg gcgcgctgtc ggatcgtttc
     5101 gggcggcggc cggtcttgct cgtctcgctg gccggcgctg ctgtcgacta cgccatcatg
     5161 gcgacggcgc ctttcctttg ggttctctat atcgggcgga tcgtggccgg catcaccggg
     5221 gcgactgggg cggtagccgg cgcttatatt gccgatatca ctgatggcga tgagcgcgcg
     5281 cggcacttcg gcttcatgag cgcctgtttc gggttcggga tggtcgcggg acctgtgctc
     5341 ggtgggctga tgggcggttt ctccccccac gctccgttct tcgccgcggc agccttgaac
     5401 ggcctcaatt tcctgacggg ctgtttcctt ttgccggagt cgcacaaagg cgaacgccgg
     5461 ccgttacgcc gggaggctct caacccgctc gcttcgttcc ggtgggcccg gggcatgacc
     5521 gtcgtcgccg ccctgatggc ggtcttcttc atcatgcaac ttgtcggaca ggtgccggcc
     5581 gcgctttggg tcattttcgg cgaggatcgc tttcactggg acgcgaccac gatcggcatt
     5641 tcgcttgccg catttggcat tctgcattca ctcgcccagg caatgatcac cggccctgta
     5701 gccgcccggc tcggcgaaag gcgggcactc atgctcggaa tgattgccga cggcacaggc
     5761 tacatcctgc ttgccttcgc gacacgggga tggatggcgt tcccgatcat ggtcctgctt
     5821 gcttcgggtg gcatcggaat gccggcgctg caagcaatgt tgtccaggca ggtggatgag
     5881 gaacgtcagg ggcagctgca aggctcactg gcggcgctca ccagcctgac ctcgatcgtc
     5941 ggacccctcc tcttcacggc gatctatgcg gcttctataa caacgtggaa cgggtgggca
     6001 tggattgcag gcgctgccct ctacttgctc tgcctgccgg cgctgcgtcg cgggctttgg
     6061 agcggcgcag ggcaacgagc cgatcgctga tcgtggaaac gataggccta tgccatgcgg
     6121 gtcaaggcga cttccggcaa gctatacgcg ccctaggagt gcggttggaa cgttggccca
     6181 gccagatact cccgatcacg agcaggacgc cgatgatttg aagcgcactc agcgtctgat
     6241 ccaagaacaa ccatcctagc aacacggcgg tccccgggct gagaaagccc agtaaggaaa
     6301 caactgtagg ttcgagtcgc gagatccccc ggaaccaaag gaagtaggtt aaacccgctc
     6361 cgatcaggcc gagccacgcc aggccgagaa cattggttcc tgtaggcatc gggattggcg
     6421 gatcaaacac taaagctact ggaacgagca gaagtcctcc ggccgccagt tgccaggcgg
     6481 taaaggtgag cagaggcacg ggaggttgcc acttgcgggt cagcacggtt ccgaacgcca
     6541 tggaaaccgc ccccgccagg cccgctgcga cgccgacagg atctagcgct gcgtttggtg
     6601 tcaacaccaa cagcgccacg cccgcagttc cgcaaatagc ccccaggacc gccatcaatc
     6661 gtatcgggct acctagcaga gcggcagaga tgaacacgac catcagcggc tgcacagcgc
     6721 ctaccgtcgc cgcgaccccg cccggcaggc ggtagaccga aataaacaac aagctccaga
     6781 atagcgaaat attaagtgcg ccgaggatga agatgcgcat ccaccagatt cccgttggaa
     6841 tctgtcggac gatcatcacg agcaataaac ccgccggcaa cgcccgcagc atcgcgaccg
     6901 tcatcggtga gaagttcggc aggtattggg tggtgacaat gtaggtgctg ccccaaatgg
     6961 caggtgctat cgctgtgaac aataaatcgg gcgtgcgtaa tgacatgcga ttacaagacc
     7021 tccgctacgg cgatgggttt ttgagagttg caggcgccgc gatgaccgca ccctgacttg
     7081 gactggccct gcctcgcacc aatccacctg cggctatggc cgcgatcatg cgaggcccga
     7141 aaattacacg acctgtacac gtccgccgaa cccggaacgc aacagagctt cgtgggcatc
     7201 ccgatccggg tcgtagcagc agtcctggac caaacgcacg tcgtagtccg catcactagc
     7261 ccaggcgacg cttgaaagaa caacgccggt ggtgcttatc ccggccatga caagcgtgct
     7321 tacgccgcgc gtccgaaggt cggcgtcgag cgccgtgcca taaaagacgc tggctcgcgg
     7381 acatgcatag aaaaggtcgc cccgttcgat cgcaagccct tcgacgggca gaccggtgcg
     7441 aaatcgtccg ctcgggagat acggtgagat ttggcgattc gtcgccggcg gcgcatgttc
     7501 atactcttcg ccgagcgaaa agttggggaa cagcacgggc cggccgcgca aggcgtgctg
     7561 gcagccgtgc agaccctgcg tgagatgaac gccgacaacc tgcgcaaggt gccggccgat
     7621 gcacccacgg ccttcatcaa gccgcgctgg aagccgctgg tgatcacccc ggaaggcctc
     7681 gaccggaaat tctacgaaat ctgcgccctg tccgagctga agaacgccct gcgctccggc
     7741 gacatctggg tcaagggctc gcggcagttc cgcgacttcg acgactacct gctgccggcc
     7801 gagaagttcg ccgcactcaa gcgcgagcag gccctgcccc tggcgatcaa cccgaacagc
     7861 gaccagtacc tggaagagcg tttgcagctg ctggacgagc agttggccac cgtcacccgc
     7921 ctggccaagg acaacgagct gcccgatgcc atcctcaccg agtcagggct gaaaatcacc
     7981 ccgctggatg cggcggtgcc ggatcgggcg caggcgctga tcgaccaaac cagccagtta
     8041 ctgccgcgca tcaagatcac cgaactgctg atggacgtgg acgactggac gggcttcagc
     8101 cgccacttca cccacttgaa ggacggggcc gaggccaaag acaggacgtt gctgctgtcc
     8161 gcaatcctcg gtgatgcgat caacctcggg ctgaccaaga tggccgagtc gagccccggc
     8221 ctgacctacg ccaagctgtc ctggctgcaa gcctggcaca tccgcgacga aacctattcg
     8281 gcggccttgg ccgagctggt caaccaccag tatcgccacg cctttgccgc ccactggggc
     8341 gacggcacga cctcatcctc cgatggccag cgcttccgcg cgggtggccg gggcgagagc
     8401 accgggcacg tcaacccgaa gtacggtagc gagccgggac ggctgttcta tacccatatc
     8461 tccgaccagt acgcgccgtt cagcacccgc gtggtgaatg tcggcgtccg cgattccacc
     8521 tatgtgctcg acggcctgct gtaccacgag tccgacctgc ggatcgagga gcactacacc
     8581 gacacggccg gcttcaccga tcacgtcttt gccctgatgc acctgctagg cttccgcttc
     8641 gcgccgcgca tccgcgacct cggcgaaacc aagctgtacg tgccgcaggg cgtgcaagcc
     8701 tacccgacgt tgcgcccgct gatcggcggc accctgaaca tcaagcacgt gcgtgcccac
     8761 tgggacgaca tcctgcgcct ggccagctcg atcaagcagg gcaccgtcac cgcctcgctg
     8821 atgctgcgca agctcggcag ctacccgcgc cagaacggac tggccgtggc cctgcgcgag
     8881 ctgggccgga tcgagcgcac gctgttcatc ctggactggc tgcaaagtgt tgaactgcgc
     8941 cgccgcgtgc atgccggcct gaacaaaggt gaggcgcgca actcgctggc cagggcggtg
     9001 ttcttcaacc gccttgggga aatcagggat cggagcttcg agcagcagcg ctaccgggcc
     9061 agcggcctca acctggtgac ggcggctatc gtgctgtgga acacggtgta cctggaacgc
     9121 gccacccagg ggttggtcga ggccggcaag ccggtggacg gcgagctgct gcaattcctg
     9181 tcgccgctgg gctgggagca catcaaccta accggcgatt acgtctggcg gcagagccgc
     9241 agactggaag acgggaagtt tcggccctta cggatgcccg gaaaacctta gcgtacgatt
     9301 ttttccgaat tctgcgggct ccccaggatg gatgaagaaa gggtgttttc actcagctat
     9361 gagcagttga cccgaatagc ggaaaaaaat atccgtgaat gtgggctgga cagccagagc
     9421 gccaggtgcc tcaatgaact gagggcctca gctctgctgt ggttgtggta tgaactggcc
     9481 attcatggtg cgccacagaa caatcacgca caggcccggg aacgcatcga taccgattat
     9541 cagcgcctca aaaaactcat ctggtcggag ggtgacagtt aatgactcag agccgccgac
     9601 cgtcgccact gcagcggaga
//
LOCUS       DAZGJT010000385         6535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.178, whole genome shotgun sequence.
ACCESSION   DAZGJT010000385 DAZGJT010000000
VERSION     DAZGJT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.178"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..1196
                     /locus_tag="ACHKFK_005142"
     CDS             72..1196
                     /locus_tag="ACHKFK_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO9055238.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            1177..3480
                     /gene="trbC"
                     /locus_tag="ACHKFK_005143"
     CDS             1177..3480
                     /gene="trbC"
                     /locus_tag="ACHKFK_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO9055239.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            4344..5159
                     /gene="sul2"
                     /locus_tag="ACHKFK_005144"
     CDS             4344..5159
                     /gene="sul2"
                     /locus_tag="ACHKFK_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HFO9055240.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            5220..6023
                     /locus_tag="ACHKFK_005145"
                     /note="aph(3'')-Ib"
     CDS             5220..6023
                     /locus_tag="ACHKFK_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HFO9055241.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            6023..6499
                     /locus_tag="ACHKFK_005146"
                     /pseudo
     CDS             6023..6499
                     /locus_tag="ACHKFK_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0019748 - secondary metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
BASE COUNT         1570 a         1825 c         1687 g         1453 t
ORIGIN      
        1 gaaaccaccg aaaccaccga aaccaccgaa accaccgaaa aacagcatcc cgccgacaag
       61 gaaaaagaac aatgaccatc cactacactg caacactgaa taataaacac ctgtccctga
      121 tcgccctgta ccctgacggt aatcgggagg tgattgccat cctgccagtc acagaactgg
      181 gcgcactgat taaccgtaat aacaggctga tgcacctgtc cttcagttcc ccttttcgcg
      241 aaataaccga aacggtgctt gttgataccg ttgatgcaga acgtcccgga catcacatcg
      301 gcagaatgct ctcagcgatt gatgcctgcc tggatgccga atatgccgga aaaggaaaac
      361 atccctcccg gaaaaatggc ttctggcgga cactgatgtg tggctttatc gccggaattg
      421 ctctgccggc gacagttgca ggttaccact ggatgaaggc agagccccct tccacgctgg
      481 cttaccctac atctcaggaa tggagagctg aaccagtaac cgacacagag acaccacgcc
      541 cacatacgga acatcatgtt aacagcatcc cggcccgccc ggctcccaca cagcaggaac
      601 cttcttctgc ccccgccccg acggcagtga cggaagaaca acacgttcag gccgctgagg
      661 cccgccgtaa tctggccaca gtacttaaac gcaacgcaga ccgggggatg ttcaccatta
      721 acctgtccac tggtcatgaa cgcacgctgt acgccttcct tgaccccgca tgcatcaact
      781 gccgtctgct ggaacccgcg cttaagcaac tggcccggga attcagcgtg gtgatttatc
      841 cggtctccgt gattggcggg gagtccagta caaagcggac tgctccgctg ctgtgtgaac
      901 cggactcaca aaaacgggcg accggatggc atcagctgta ttccgctgac agtggtatgg
      961 cccccaccgg cgatgcaccg gccgcacagg atggacagtg tctgaccgcc gcacgtgctg
     1021 ccatcgatgt caataaccag gctttccgcc agttcggttt tggcggaacc ccctgggtgt
     1081 tatcagatac cggctggcac ctgccgtccg gcatcctgaa agacgccact tccgttcgcc
     1141 tcttcctgaa agcaacagat agcgaggagt atcacaatga gccagcacca cgttaatcct
     1201 gacctcatac accgcacggc ctggggaaac ccgttgtgga atgcactgca taacctgaac
     1261 atcactggtc tgtgtctggc cggcagtatc attacggcgc tgatatggcc actggccctt
     1321 cctgtctgcc tgctgttcac cctggtcacc tccgtgatat tcatgctgca acgctggcga
     1381 tgccccctgc gtatgcccat gacactcagc cttgatgacc cctcccagga cagaaaggtc
     1441 aggagaagcc tcttcagctt ctggcccacc ctgtttcagt atgaagcgga tgaaacattc
     1501 cccgcccggg ggatttttta tgtcggttac cgccgtatca acgatatcgg tcgtgagctc
     1561 tggctgagca tggatgacct cacccggcat gtcatgtttt ttgccaccac cggtggcggt
     1621 aaaacagaaa ccacctttgc ctggctgctc aacccgctct gctggggaag aggctttacc
     1681 tttgttgacg gaaaggcaca gaacgatacc acgaggacaa tctggtatct ctcccgacgt
     1741 tttggcaggg aggatgatat cgaagtcatc aacttcatga acggcggaaa atcccgcagt
     1801 gaaatcattc agagcggcga aaaatcccgc ccacagtcaa acacctggaa cccgttcgca
     1861 ttcagcaccg aagccttcac cgccgaaacc atgcagtcca tgctgcccca gaacgttcag
     1921 ggcggtgaat ggcagtcacg ggcgatagcc atgaacaagg ccctggtctt tggcacaaaa
     1981 ttctggtgcg tccgggaagg taaaaccatg tccctgcaga tgttacggga acatatgacg
     2041 ctggaaggga tggcaaaact ctattgccgt ggccttgatg accagtggcc ggaagaagct
     2101 atcgcaccac tgcgaaacta cctgcaggat gtcccgggat tcgatatgtc actggtgaga
     2161 acgccgtcag cctggactga ggagccccgt aaacagcatg cttatctctc cggtcagttc
     2221 tcggaaacgt tcacaacctt tgccgaaacc ttcggggacg tctttgcggc agatgccggc
     2281 gacatcgata ttcgcgacag tattcacagc gaccgcattc tgattgtgat gatccctgca
     2341 ctggacacat cggctcatac cacgtcggca ctcggtcgta tgttcgtcac ccagcagagc
     2401 atgctcctcg cccgtgacct gggctaccgg ctggaaggca cagatgcaca gaccctggaa
     2461 gtcaaaaaat acaaaggcag cttcccctat atctgtatcc ttgacgaagt cggtgcctac
     2521 tacacagacc gcattgctgt ggaggccacc caggtacgtt cactggagtt ttccctgata
     2581 atgacggcgc aggatcagga gcgaattgaa gggcagacct ccgccaccaa caccgccaca
     2641 ctgatgcaga acgccggcac caaattcgcc ggacgcattg tcagtgacga caaaacggca
     2701 agaacggtga aaaatgccgc cggtgaagag gcacgggcca gaatgggcag cctgcagcgc
     2761 cacgatggtg tgatgggtga gtcctgggtg gacggtaacc agatcaccat ccagatggaa
     2821 agcaaaatcg acgtgcagga tttgatcaga ctgaatgctg gtgagttctt taccgttttc
     2881 cagggggatg tggtgccgtc agcctctgtg tatattccgg acagcgagaa aagttgcgac
     2941 agtgatccgg tggtcatcaa ccgctatatt tccgttgaag ccccccggct ggagcgtctc
     3001 cgccgtctcg tccccaggac agtgcagcgc aggctcccca caccggagca tgtcagcagc
     3061 atcatcggtg tactgacagc aaaaccatcc cgtaaacgtc gtaaaaaccg tacagagcct
     3121 taccgtatca ttgatacatt ccagcgccag ttggcaacag cgcagacatc actggatctg
     3181 cttccgcaat atgatacaga tatcgaatcc cgggcaaacg aactgtggaa aaaggctgtg
     3241 cacactatca ataacacaac cagggaagaa cgccgggtat gttatatcac actgaatcgt
     3301 ccggaagagg aacactccgg tcctgaagat ataccttcag tgaaggcgat tctgaatact
     3361 ctgctccccc tggagatgct gttacctgta ccggatctaa ctgcatcccc gccccataaa
     3421 aaaaatgtcg ctcagacacc atcaggtgga aaacaagaga gtcgtaaaaa aagattctga
     3481 taacacctgt atggctgatt ttaataacaa cctttacgcg attattaatc tcctccgctc
     3541 accatagtaa gcggaggatt caggatttca atccatcacc agaggtacaa taaatgtgta
     3601 ttagtagccc ggaaacaaac tcatggtctg ttatttatcg taaaaattcc ggagaagata
     3661 tcaatatcac ttccctgacg tttaaaaata gtcttctggc agcccggaca ctgatggtgc
     3721 ccgaaaacta tatgatatgc atactcagaa acggggagag ggtcagacga tgggacagag
     3781 aaatacttgc aggctcaaac agatggtaca agtgttctcc cgataatttc gaaatactgg
     3841 gtaagctccc cataataaat aaagtcacaa aactgataaa atcataatct acggatttta
     3901 atcagttaag ctaaatcagg atgaacgctt tgattatgaa ttatacatga aaacaaatat
     3961 gggcagcaca aaaaatggtg ccagggttcc ccacgttcgt aatagattca cttctatgga
     4021 tattggtatg aattcccaga tcccactcta ttgtaaacaa gacattttta tcttttatat
     4081 tcaatggctt attttcctgc taattggtaa taccatgaaa aataccatgc tcagaaaagg
     4141 cttaacaata ttttgaaaaa ttgcctactg agcgctgccg cacagctcca taggccgctt
     4201 tcctggcttt gcttccagat gtatgctatt ctgctcctgc agctaatgga tcaccgcaaa
     4261 caggttactc gcctggggat tccctttcga cccgagcatc cgtatgagac tcatgctcga
     4321 ttattattat tatagaagcc cccatgaata aatcgctcat cattttcggc atcgtcaaca
     4381 taacctcgga cagtttctcc gatggaggcc ggtatctggc gccagacgca gccattgcgc
     4441 aggcgcgtaa gctgatggcc gagggggcag atgtgatcga cctcggtccg gcatccagca
     4501 atcccgacgc cgcgcctgtt tcgtccgaca cagaaatcgc gcgtatcgcg ccggtgctgg
     4561 acgcgctcaa ggcagatggc attcccgtct cgctcgacag ttatcaaccc gcgacgcaag
     4621 cctatgcctt gtcgcgtggt gtggcctatc tcaatgatat tcgcggtttt ccagacgctg
     4681 cgttctatcc gcaattggcg aaatcatctg ccaaactcgt cgttatgcat tcggtgcaag
     4741 acgggcaggc agatcggcgc gaggcacccg ctggcgacat catggatcac attgcggcgt
     4801 tctttgacgc gcgcatcgcg gcgctgacgg gtgccggtat caaacgcaac cgccttgtcc
     4861 ttgatcccgg catggggttt tttctggggg ctgctcccga aacctcgctc tcggtgctgg
     4921 cgcggttcga tgaattgcgg ctgcgcttcg atttgccggt gcttctgtct gtttcgcgca
     4981 aatcctttct gcgcgcgctc acaggccgtg gtccggggga tgtcggggcc gcgacactcg
     5041 ctgcagagct tgccgccgcc gcaggtggag ctgacttcat ccgcacacac gagccgcgcc
     5101 ccttgcgcga cgggctggcg gtattggcgg cgctgaaaga aaccgcaaga attcgttaac
     5161 tgcacattcg ggatatttct ctatattcgc gcttcatcag aaaactgaag gaacctccat
     5221 tgaatcgaac taatattttt tttggtgaat cgcattctga ctggttgcct gtcagaggcg
     5281 gagaatctgg tgattttgtt tttcgacgtg gtgacgggca tgccttcgcg aaaatcgcac
     5341 ctgcttcccg ccgcggtgag ctcgctggag agcgtgaccg cctcatttgg ctcaaaggtc
     5401 gaggtgtggc ttgccccgag gtcatcaact ggcaggagga acaggagggt gcatgcttgg
     5461 tgataacggc aattccggga gtaccggcgg ctgatctgtc tggagcggat ttgctcaaag
     5521 cgtggccgtc aatggggcag caacttggcg ctgttcacag cctatcggtt gatcaatgtc
     5581 cgtttgagcg caggctgtcg cgaatgttcg gacgcgccgt tgatgtggtg tcccgcaatg
     5641 ccgtcaatcc cgacttctta ccggacgagg acaagagtac gccgcagctc gatcttttgg
     5701 ctcgtgtcga acgagagcta ccggtgcggc tcgaccaaga gcgcaccgat atggttgttt
     5761 gccatggtga tccctgcatg ccgaacttca tggtggaccc taaaactctt caatgcacgg
     5821 gtctgatcga ccttgggcgg ctcggaacag cagatcgcta tgccgatttg gcactcatga
     5881 ttgctaacgc cgaagagaac tgggcagcgc cagatgaagc agagcgcgcc ttcgctgtcc
     5941 tattcaatgt attggggatc gaagcccccg accgcgaacg ccttgccttc tatctgcgat
     6001 tggaccctct gacttggggt tgatgttcat gccgcctgtt tttcctgctc attggcacgt
     6061 ttcgcaacct gttctcattg cggacacctt ttccagcctc gtttggaaag tttcattgcc
     6121 agacgggact cctgcaatcg tcaagggatt gaaacctata gaagacattg ctgatgaact
     6181 gcgcggggcc gactatctgg tatggcgcaa tgggagggga gcagtccggt tgctcggtcg
     6241 tgagaacaat ctgatgttgc tcgaatatgc cggggagcga atgctctctc acatcgttgc
     6301 cgagcacggc gactaccagg cgaccgaaat tgcagcggaa ctaatggcga agctgtatgc
     6361 cgcatctgag gaacccctgc cttctgccct tctcccgatc cgggatcgct ttgcagcttt
     6421 gtttcagcgg gcgcgcgatg atcaaaacgc aggttgtcaa actgactacg tccacgcggc
     6481 gattatagcc gatcaaatgt cacgaacggt gcaatagtga tccacaccca acgcc
//
LOCUS       DAZGJT010000386         5693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.186, whole genome shotgun sequence.
ACCESSION   DAZGJT010000386 DAZGJT010000000
VERSION     DAZGJT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.186"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(175..819)
                     /locus_tag="ACHKFK_005147"
                     /pseudo
     CDS             complement(175..819)
                     /locus_tag="ACHKFK_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011918924.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
     gene            complement(896..1246)
                     /locus_tag="ACHKFK_005148"
                     /pseudo
     CDS             complement(896..1246)
                     /locus_tag="ACHKFK_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004636771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
     gene            complement(1449..2462)
                     /gene="intI1"
                     /locus_tag="ACHKFK_005149"
     CDS             complement(1449..2462)
                     /gene="intI1"
                     /locus_tag="ACHKFK_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012077405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HFO9055242.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2619..3092
                     /gene="dfrA1"
                     /locus_tag="ACHKFK_005150"
     CDS             2619..3092
                     /gene="dfrA1"
                     /locus_tag="ACHKFK_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HFO9055243.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVVIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            3185..4023
                     /gene="aadA1"
                     /locus_tag="ACHKFK_005151"
                     /pseudo
     CDS             3185..4023
                     /gene="aadA1"
                     /locus_tag="ACHKFK_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_048608579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
     gene            4140..4487
                     /locus_tag="ACHKFK_005152"
     CDS             4140..4487
                     /locus_tag="ACHKFK_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter QacE delta 1"
                     /protein_id="HFO9055244.1"
                     /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAF
                     YFLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLAR
                     SPSWKSLRRPTPW"
     gene            4481..5320
                     /gene="sul1"
                     /locus_tag="ACHKFK_005153"
     CDS             4481..5320
                     /gene="sul1"
                     /locus_tag="ACHKFK_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul1"
                     /protein_id="HFO9055245.1"
                     /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDV
                     GPAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLND
                     IQGFPDPALYPDIAEADCRLVVMHSAQRDGIATRTGHLRPEDALDEIVRFFEARVSAL
                     RRSGVAADRLILDPGMGFFLSPAPETSLHVLSNLQKLKSALGLPLLVSVSRKSFLGAT
                     VGLPVKDLGPASLAAELHAIGNGADYVRTHAPGDLRSAITFSETLAKFRSRDARDRGL
                     DHA"
     gene            5448..5693
                     /locus_tag="ACHKFK_005154"
                     /pseudo
     CDS             5448..5693
                     /locus_tag="ACHKFK_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004194535.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
BASE COUNT         1329 a         1564 c         1539 g         1261 t
ORIGIN      
        1 accatcatac actaaatcag taagttggca gcatcaccta ggattacagg ggcattcagt
       61 tgaaaggcgg cccagccact ggcatcacat acagcggatg tcacatttga accgtcaata
      121 caagcaatca catttgtcat cgttttattc ctttgttgtt gtgttgctaa tggcttagtg
      181 accagacatc attttttcaa cttcttctgg cttatcgtgt accccaaatt tatcgactat
      241 ggtggcactg gcttcgttaa ggccaatcaa ctcaacctca gtcccctcgc ggcggaattt
      301 aatcactacc ttgtcgaggg ccgataccgc ggtaatatcc caaaaatgtg cttgagataa
      361 gtcgatagtc actttatcaa ccacttccct aaaatcgaat gaattactga acttatctga
      421 tgaagcaaaa aagacttgtc caataacctg ataatggcga ccagtgtcag cagcagtcgt
      481 tgtactctta accaccataa aacgtcccac tttattagca aaaaataggg acgcgagtaa
      541 tacacctaca aataccccaa ttgctaagtt atgggtagcg accacgacca caaccgtcgc
      601 caccatcaca aggttggttg acataggatg atgcttaaga ttacggattg aatcccaaga
      661 gaatgtaccg atagccacca tgatcatcac ggcaaccagt gccgccatag gaataagctt
      721 taaccactct cctaaaaaca ccaccatcac aagtaaaaaa acgcctgcag cgaaggatga
      781 taaacgtccg cgaccacccg atttaacgtt gatgatcgaa tgtcaataaa ttcaaaattc
      841 aatcctatcc tgacgcaatt tacacatggc atctgacatc aggttagggt atgcctcaac
      901 ctgacggcgg cgaatcacaa gcgtccggtt tgacgctggt ttcggtcgca gtgctggccc
      961 gcgcctggaa gcgctgatag cactccagcc cgcagaaatg ctccacgtac tcggcccctt
     1021 ccggcgtgaa ggcggcatcg agcgggattt ccttgcagca cacgcagcaa ctggtgctcg
     1081 gttcattggc gttcatggtg gtgttcctcc attggttgac gaagccgacg aaggccgccg
     1141 ccggcatcgg cctggcgaac aggaaaccct gcaccgtgtc gcaacccgcc tgccgcaacc
     1201 acgcaaggca gtcgggtgtt tccacaccct cggctaccac ctccattgtc gttttcagaa
     1261 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatccat
     1321 caggcaacga cgggctgctg ccggccatca gcggacgcag ggaggacttt ccgcaaccgg
     1381 ccgttcgatg cggcaccgat ggccttcgcg caggggtagt gaatccgcca ggattgactt
     1441 gcgctgccct acctctcact agtgaggggc ggcagcgcat caagcggtga gcgcactccg
     1501 gcaccgccaa ctttcagcac atgcgtgtaa atcatcgtcg tagagacgtc ggaatggccg
     1561 agcagatcct gcacggttcg aatgtcgtaa ccgctgcgga gcaaggccgt cgcgaacgag
     1621 tggcggaggg tgtgcggtgt ggcgggcttc gtgatgcctg cttgttctac ggcacgtttg
     1681 aaggcgcgct gaaaggtctg gtcatacatg tgatggcgac gcacgacacc gctccgtgga
     1741 tcggtcgaat gcgtgtgctg cgcaaaaacc cagaaccacg gccaggaatg cccggcgcgc
     1801 ggatacttcc gctcaagggc gtcgggaagc gcaacgccgc tgcggccctc ggcctggtcc
     1861 ttcagccacc atgcccgtgc acgcgacagc tgctcgcgca ggctgggtgc caagctctcg
     1921 ggtaacatca aggcccgatc cttggagccc ttgccctccc gcacgatgat cgtgccgtga
     1981 tcgaaatcca gatccttgac ccgcagttgc aaaccctcac tgatccgcat gcccgttcca
     2041 tacagaagct gggcgaacaa acgatgctcg ccttccagaa aaccgaggat gcgaaccact
     2101 tcatccgggg tcagcaccac cggcaagcgc cgcgacggcc gaggtcttcc gatctcctga
     2161 agccagggca gatccgtgca cagcaccttg ccgtagaaga acagcaaggc cgccaatgcc
     2221 tgacgatgcg tggagaccga aaccttgcgc tcgttcgcca gccaggacag aaatgcctcg
     2281 acttcgctgc tgcccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga
     2341 acccagtgga cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca
     2401 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2461 atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg
     2521 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     2581 agtcgcccta aaacaaagtt aacctctgag gaagaattgt gaaactatca ctaatggtag
     2641 ctatatcgaa gaatggagtt atcgggaatg gccctgatat tccatggagt gccaaaggtg
     2701 aacagctcct gtttaaagct attacctata accaatggct gttggttgga cgcaagactt
     2761 ttgaatcaat gggagcatta cccaaccgaa agtatgcggt cgtaacacgt tcaagtttta
     2821 catctgacaa tgagaacgta gtgatctttc catcaattaa agatgcttta accaacctaa
     2881 agaaaataac ggatcatgtc attgtttcag gtggtgggga gatatacaaa agcctgatcg
     2941 atcaagtaga tacactacat atatctacaa tagacatcga gccggaaggt gatgtttact
     3001 ttcctgaaat ccccagcaat tttaggccag tttttaccca agacttcgcc tctaacataa
     3061 attatagtta ccaaatctgg caaaagggtt aacaagtggc agcaacggat tcgcaaacct
     3121 gtcacgcctt ttgtaccaaa agccgcgcca ggtttgcgat ccgctgtgcc aggcgttaaa
     3181 catcatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg tagttggcgt
     3241 catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct ccgcagtgga
     3301 tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg taaggcttga
     3361 tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt cccctggaga
     3421 gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca tcattccgtg
     3481 gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg acattcttgc
     3541 aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga caaaagcaag
     3601 agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc cggttcctga
     3661 acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc cgcccgactg
     3721 ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca gcgcagtaac
     3781 cggcagaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc tgccggccca
     3841 gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag aagatcgctt
     3901 ggcctcgcgc gcagatcagt tggaagaatt tgttcactac gtgaaaggcg agatcaccaa
     3961 ggtagtcggc aaataatgtc taacaattcg ttcaagccga cgccgcttcg cggcgcggct
     4021 taactcaagc gttagatgca ctaagcacat aattgctcac agccaaacta tcaggtcaag
     4081 tctgctttta ttatttttaa gcgtgcataa taagccctac acaaattggg agatatatca
     4141 tgaaaggctg gctttttctt gttatcgcaa tagttggcga agtaatcgca acatccgcat
     4201 taaaatctag cgagggcttt actaagcttg ccccttccgc cgttgtcata atcggttatg
     4261 gcatcgcatt ttattttctt tctctggttc tgaaatccat ccctgtcggt gttgcttatg
     4321 cagtctggtc gggactcggc gtcgtcataa ttacagccat tgcctggttg cttcatgggc
     4381 aaaagcttga tgcgtggggc tttgtaggta tggggctcat aattgctgcc tttttgctcg
     4441 cccgatcccc atcgtggaag tcgctgcgga ggccgacgcc atggtgacgg tgttcggcat
     4501 tctgaatctc accgaggact ccttcttcga tgagagccgg cggctagacc ccgccggcgc
     4561 tgtcaccgcg gcgatcgaaa tgctgcgagt cggatcagac gtcgtggatg tcggaccggc
     4621 cgccagccat ccggacgcga ggcctgtatc gccggccgat gagatcagac gtattgcgcc
     4681 gctcttagac gccctgtccg atcagatgca ccgtgtttca atcgacagct tccaaccgga
     4741 aacccagcgc tatgcgctca agcgcggcgt gggctacctg aacgatatcc aaggatttcc
     4801 tgaccctgcg ctctatcccg atattgctga ggcggactgc aggctggtgg ttatgcactc
     4861 agcgcagcgg gatggcatcg ccacccgcac cggtcacctt cgacccgaag acgcgctcga
     4921 cgagattgtg cggttcttcg aggcgcgggt ttccgccttg cgacggagcg gggtcgctgc
     4981 cgaccggctc atcctcgatc cggggatggg atttttcttg agccccgcac cggaaacatc
     5041 gctgcacgtg ctgtcgaacc ttcaaaagct gaagtcggcg ttggggcttc cgctattggt
     5101 ctcggtgtcg cggaaatcct tcttgggcgc caccgttggc cttcctgtaa aggatctggg
     5161 tccagcgagc cttgcggcgg aacttcacgc gatcggcaat ggcgctgact acgtccgcac
     5221 ccacgcgcct ggagatctgc gaagcgcaat caccttctcg gaaaccctcg cgaaatttcg
     5281 cagtcgcgac gccagagacc gagggttaga tcatgcctag cattcacctt ccggccgccc
     5341 gctagcggac cctggtcagg ttccgcgaag gtgggcgcag acatgctggg ctcgtcagga
     5401 tcaaactgca ctatgaggcg gcggttcata ccgcgccagg ggagcgaatg gacagcgagg
     5461 agcctccgaa cgttcgggtc gcctgctcgg gtgatatcga cgaggttgtg cggctgatgc
     5521 acgacgctgc ggcgtggatg tccgccaagg gaacgccgcc tgggacgtcg cgcggatcga
     5581 ccggacattc gcggagacct tcgtcctgag atccgagctc ctagtcgcga gttgcagcga
     5641 cggcatcgtc ggctgttgca ccttgtcggc cgaggatccc gagttctggc ccg
//
LOCUS       DAZGJT010000387         1881 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.261, whole genome shotgun sequence.
ACCESSION   DAZGJT010000387 DAZGJT010000000
VERSION     DAZGJT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.261"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..691
                     /locus_tag="ACHKFK_005155"
     CDS             134..691
                     /locus_tag="ACHKFK_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005687610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO9055246.1"
                     /translation="MRLFGYARVSTSQQSLDLQVRALKDAGVKANRIFTDKASGSSTD
                     REGLDLLRMKVEEGDVILVKKLDRLGRDTADMIQLIKEFDAQGVAVRFIDDGISTDGD
                     MGQMVVTILSAVAQAERRRILERTNEGRQEAKLKGIKFGRRRTVDRNVVLTLHQKGTG
                     ATEIAHQLSIARSTVYKILEDERAS"
     gene            874..1734
                     /locus_tag="ACHKFK_005156"
     CDS             874..1734
                     /locus_tag="ACHKFK_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032489893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="broad-spectrum class A beta-lactamase TEM-1"
                     /protein_id="HFO9055247.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
BASE COUNT          515 a          403 c          465 g          498 t
ORIGIN      
        1 aacatttttg caaccgtacg aaatgttata aattatcgga catcgtaaaa ctgttacatt
       61 aatatgtcta ttaaatcgta aatttgtaat aatagacatg agttgtccga tattcgattt
      121 aaggtacatt tttatgcgac tttttggtta cgctcgggtc tcaaccagtc agcagtctct
      181 tgatcttcag gtcagagcac tcaaagacgc aggtgtgaaa gcaaaccgta tatttaccga
      241 taaggcatcc ggcagttcaa cagatcggga agggctggat ttgctgagga tgaaggtgga
      301 ggaaggtgat gtcattctgg ttaagaagct cgaccgtctt ggccgcgaca ctgccgatat
      361 gatccaactg ataaaggaat ttgacgctca gggcgtggca gtccggttca ttgatgacgg
      421 gatcagtacc gacggtgata tggggcaaat ggtggtcacc atcctgtcgg ctgtggcaca
      481 ggctgaacgc cggaggatcc tagagcgcac gaatgagggc cgacaggaag caaagctgaa
      541 aggaatcaaa tttggccgca ggcgtaccgt ggacaggaac gtcgtgctga cgcttcatca
      601 gaagggcact ggtgcaacgg aaattgctca tcagctcagt attgcccgct ccacggttta
      661 taaaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
      721 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
      781 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      841 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      901 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
      961 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1021 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1081 tttaaagttc tgctatgtgg tgcggtatta tcccgtgttg acgccgggca agagcaactc
     1141 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1201 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1261 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1321 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1381 gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     1441 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1501 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1561 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1621 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1681 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1741 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1801 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1861 ttccactgag cgtcagaccc c
//
LOCUS       DAZGJT010000388         1615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2002M67546
            SAMN43267205-rid23071773.guided.273, whole genome shotgun sequence.
ACCESSION   DAZGJT010000388 DAZGJT010000000
VERSION     DAZGJT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267205
            Sequence Read Archive: SRR30307736
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351053.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351053.1
            Genome Coverage       :: 128x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 02:29:20
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,158
            CDSs (total)                      :: 5,070
            Genes (coding)                    :: 4,807
            CDSs (with protein)               :: 4,807
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 4 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 4 (16S, 23S)
            tRNAs                             :: 76
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 263
            CDSs (without protein)            :: 263
            Pseudo Genes (ambiguous residues) :: 0 of 263
            Pseudo Genes (frameshifted)       :: 109 of 263
            Pseudo Genes (incomplete)         :: 148 of 263
            Pseudo Genes (internal stop)      :: 57 of 263
            Pseudo Genes (multiple problems)  :: 44 of 263
            Pseudo Genes (short protein)      :: 1 of 263
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267205-rid23071773.guided.273"
                     /serotype="O117:H 7"
                     /isolate="2002M67546"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2020-02"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..1189
                     /gene="stxA1a"
                     /locus_tag="ACHKFK_005157"
                     /pseudo
     CDS             241..1189
                     /gene="stxA1a"
                     /locus_tag="ACHKFK_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
     gene            1199..1468
                     /gene="stxB1a"
                     /locus_tag="ACHKFK_005158"
     CDS             1199..1468
                     /gene="stxB1a"
                     /locus_tag="ACHKFK_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO9055248.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          436 a          271 c          392 g          516 t
ORIGIN      
        1 ggctggcttt tttatgtccg taacatcctg tgtatcaata aatgttgttg tctacgtacg
       61 tcaagtagcc gcatgagatc tgaccagata tgttaaggtt gcagctctct ttgaatataa
      121 ttatcatttt cattacgtta ttgttacgtt tatccggtgc gccgtaaaac gccgtccttc
      181 agggggtgga ggatgtcaag aatatagtta tcgtatggtg ctcaaggagt attgtgtaat
      241 atgaaaataa ttatttttag agtgctaact tttttctttg ttatcttttc agttaatgtg
      301 gttgcgaagg aatttacctt agacttctcg actgcaaaga cgtatgtaga ttcgctgaat
      361 gtcattcgct ctgcaatagg tactccatta cagactattt catcaggagg tacgtcttta
      421 ctgatgattg atagtggcac aggggataat ttgtttgcag ttgatgtcag agggatagat
      481 ccagaggaag ggcggtttaa taatctacgg cttattgttg aacgaaataa tttatatgtg
      541 acaggatttg ttaacaggac aaataatgtt tttttatcgc tttgctgatt tttcacatgt
      601 tacctttcca ggtacaacag cggttacatt gtctggtgac agtagctata ccacgttaca
      661 gcgtgttgca gggatcagtc gtacggggat gcagataaat cgccattcgt tgactacttc
      721 ttatctggat ttaatgtcgc atagtggaac ctcactgacg cagtctgtgg caagagcgat
      781 gttacggttt gttactgtga cagctgaagc tttacgtttt cggcaaatac agaggggatt
      841 tcgtacaaca ctggatgatc tcagtgggcg ttcttatgta atgactgctg aagatgttga
      901 tcttacattg aactggggaa ggttgagtag tgtcctgcct gactatcatg gacaagactc
      961 tgttcgtgta ggaagaattt cttttggaag cattaatgca attctgggaa gcgtggcatt
     1021 aatactgaat tgtcatcatc atgcatcgcg agttgccaga atggcatctg atgagtttcc
     1081 ttctatgtgt ccggcagatg gaagagtccg tgggattacg cacaataaaa tattgtggga
     1141 ttcatccact ctgggggcaa ttctgatgcg cagaactatt agcagttgag ggggtaaaat
     1201 gaaaaaaaca ttattaatag ctgcatcgct ttcatttttt tcagcaagtg cgctggcgac
     1261 gcctgattgt gtaactggaa aggtggagta tacaaaatat aatgatgacg atacctttac
     1321 agttaaagtg ggtgataaag aattatttac caacagatgg aatcttcagt ctcttcttct
     1381 cagtgcgcaa attacgggga tgactgtaac cattaaaact aatgcctgtc ataatggagg
     1441 gggattcagc gaagttattt ttcgttgact cagaatagct cagtgaaaat agcaggcgga
     1501 gattcataaa tgttaaatac atctcaattc agtcagttgt tgccggtctg ataatagatg
     1561 tgttagaaaa tttctgcatg gtgaatcccc ctgtgcggag gggcgactgg tgaac
//
